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Sample records for total coliform levels

  1. Bacteriological (fecal and total coliform) quality of Pakistani coastal water

    The coliform bacteria group consists of several genera of bacteria belonging to the family enterobacteriaceae. These are harmless bacteria, mostly live in soil, water, and digestive system of animals. Fecal coliform bacteria, which belongs to this group, are present in large numbers in feces and intestinal tract of human beings and other warm-blooded animals which can enter into water bodies from human and animal waste. Swimming in water having high levels of Fecal coliform bacteria increases the chance of developing illness (fever, nausea or stomach cramps) from pathogens entering the body through mouth, nose, ears or cuts in the skin. The objective of the present study was to characterize the bathing quality of Pakistani coastal water with respect to coliform bacteria. Total and Fecal coliform bacteria were determined at seven different locations along Pakistan coast using membrane filtration (MF) technique. 100 ml of water was passed through 0.45 micron (mu) filter paper. These filter papers were put on pads, soaked in Lauryle sulphate broth in petri-dishes and incubated at 44 deg. C for Fecal and 37 deg. for Total coliform for 24 hours. Significantly high population of Fecal and Total coliform bacteria was recorded at Karachi harbour area and Indus delta region. Results indicate that a large amount of domestically originated waste is being discharged into these locations without any pre-treatment (e.g., screening, activated sludge, by using filtration beds etc.) resulting in a poor seawater quality making it unfit for bathing. (author)

  2. Evaluation of Peel Plate™ EC for Determination of E. coli and Coliform or Total Coliform in Dairy Products.

    Salter, Robert S; Durbin, Gregory W; Bird, Patrick; Fisher, Kiel; Crowley, Erin; Chen, Yi; Clark, Dorn; Ziemer, Wayne

    2016-01-01

    Peel Plate™ EC is a low-profile plastic, 47 mm culture dish with an adhesive top that contains a dried medium with Gram-negative selective agents and with enzyme substrate indicators for β-galactosidase (coliform) and β-glucuronidase (Escherichia coli). The method provides a conventional quantitative coliform (red) and E. coli (blue/purple/black) count with simple rehydration and incubation for 24 ± 2 h at 35 ± 1°C, while providing a total coliform result, sum of E. coli, and coliform without color differential in dairy products at 32 ± 1°C for 24 ± 2 h. Dairy matrixes claimed and supported with total coliform data are whole milk, skim milk, chocolate milk (2% fat), heavy cream (35% fat), pasteurized whole goat milk, ultra-high-temperature pasteurized milk, powdered milk, lactose-reduced milk, strawberry milk, shredded cheddar cheese, raw cow milk, raw goat milk, raw sheep milk, sour cream, condensed milk, eggnog, vanilla ice cream, condensed whey, yogurt, and cottage cheese. Matrixes claimed for E. coli and total coliform detection are raw ground beef, mixed cellulose 0.45 μm filtered bottled water, environmental sponge of stainless steel, raw ground turkey, dry dog food, liquid whole pasteurized eggs, milk chocolate, leafy green (mixed greens) rinse/flume water, irrigation water, poultry carcass rinse, and large animal carcass sponge. The method has been independently evaluated for total coliform in whole milk, skim milk, chocolate milk, and heavy cream. The method was also independently evaluated for E. coli and coliform in ground beef, filtered bottled water, and sponge rinse from stainless steel surfaces. In inclusivity and exclusivity studies, the method detected 57 of 58 different strains of coliform and E. coli at 32 ± 1°C and 35 ± 1°C in and excluded 31 of 32 different noncoliform strains consisting of Gram-negative and Gram-positive bacteria. In the matrix study, each matrix was assessed separately at each contamination level in comparison

  3. Comparison of Verification Procedures for the Membrane Filter Total Coliform Technique

    LeChevallier, Mark W.; Cameron, Susan C.; McFeters, Gordon A.

    1983-01-01

    Verification of membrane filter total coliform colonies from drinking water was increased 87% by testing for the presence of β-galactosidase and cytochrome oxidase, compared with verification by determination of gas production in lauryl tryptose broth. Over 90% of the coliforms verified by testing for β-galactosidase and cytochrome oxidase were representative of the typical coliform genera.

  4. 75 FR 49930 - Stakeholder Meeting Regarding Re-Evaluation of Currently Approved Total Coliform Analytical Methods

    2010-08-16

    ... From the Federal Register Online via the Government Publishing Office ENVIRONMENTAL PROTECTION AGENCY Stakeholder Meeting Regarding Re-Evaluation of Currently Approved Total Coliform Analytical...) analytical methods. At these meetings, stakeholders will be given an opportunity to discuss...

  5. 75 FR 18205 - Total Coliform Rule Revisions-Notice of Stakeholder Meeting

    2010-04-09

    ... AGENCY Total Coliform Rule Revisions--Notice of Stakeholder Meeting AGENCY: Environmental Protection... the TCR. One of the recommendations of the Committee is that EPA conduct stakeholder outreach as the... Systems Research and Information Collection Partnership; opportunities for stakeholders to...

  6. Bacteria holding times for fecal coliform by mFC agar method and total coliform and Escherichia coli by Colilert®-18 Quanti-Tray® method

    Aulenbach, Brent T.

    2010-01-01

    Bacteria holding-time experiments of up to 62 h were performed on five surface-water samples from four urban stream sites in the vicinity of Atlanta, GA, USA that had relatively high densities of coliform bacteria (Escherichia coli densities were all well above the US Environmental Protection Agency criterion of 126 colonies (100 ml) − 1 for recreational waters). Holding-time experiments were done for fecal coliform using the membrane filtration modified fecal coliform (mFC) agar method and for total coliform and E. coli using the Colilert®-18 Quanti-Tray® method. The precisions of these analytical methods were quantified. Precisions determined for fecal coliform indicated that the upper bound of the ideal range of counts could reasonably be extended upward and would improve precision. For the Colilert®-18 method, analytical precisions were similar to the theoretical precisions for this method. Fecal and total coliform densities did not change significantly with holding times up to about 27 h. Limited information indicated that fecal coliform densities might be stable for holding times of up to 62 h, whereas total coliform densities might not be stable for holding times greater than about 27 h. E. coli densities were stable for holding times of up to 18 h—a shorter period than indicated from a previous studies. These results should be applicable to non-regulatory monitoring sampling designs for similar urban surface-water sample types.

  7. Application of a good manufacturing practices checklist and enumeration of total coliform in swine feed mills

    Debora da Cruz Payao Pellegrini

    2014-02-01

    Full Text Available A cross-sectional study in four swine feed mills aimed to evaluate the correlation between the score of the inspection checklist defined in the Normative Instruction 4 (IN 4/ Brazilian Ministry of Agriculture, Livestock and Food Supply, and the enumeration of total coliforms throughout the manufacturing process. The most of non-conformities was found in the physical structure of the feed mills. Feed mill B showed the lowest number of unconformities while units A and D had the largest number of nonconformities. In 38.53% (489/1269 of the samples the presence of total coliform was detected, however no significant difference in the bacterial counts was observed between sampling sites and feed mills. The logistic regression pointed higher odds ratio (OR for total coliforms isolation at dosing (OR = 9.51, 95% CI: 4.43 to 20.41, grinding (OR = 7.10, 95% CI = 3.27 to 15.40 and residues (OR = 6.21, 95% CI: 3.88 to 9.95 In spite of having the second score in the checklist inspection, feed mill C presented the highest odds for total coliforms isolation (OR= 2,43, IC 95%: 1,68-3,53. The data indicate no association between the score of checklist and the presence of hygienic indicators in feed mills.

  8. Comparison of media for recovery of total coliform bacteria from chemically treated water.

    Rice, E W; Fox, K R; Nash, H D; Read, E.J.; Smith, A P

    1987-01-01

    Five broth media and two solid media were compared for their ability to quantitatively recover total coliform bacteria from chemically treated water. M-Endo LES and mT7 media were used in the membrane filter technique. Lauryl tryptose broth, lactose broth, presence-absence broth, lactose broth with twice the amount of lactose, and lauryl tryptose broth with twice the amount of sodium lauryl sulfate were used in the fermentation tube procedure. The differences in recovery were not significant ...

  9. ANALYSIS OF TOTAL AND FECAL COLIFORMS ON IRRIGATION WATERS OF VEGETABLE GARDENS IN SINOP-MT, BRAZIL

    P. D. S. Batista

    2015-02-01

    Full Text Available The consumption of raw food such as vegetables and fruits, is a practice that causes several times in the contamination of the intestinal tract. The water used for irrigation of the vegetable gardens represents a possible source of contamination of the product, compromising human health. This study aimed to evaluate the bacteriological quality of the water of irrigation used in vegetable gardens of Sinop-MT. It was determined the most probable number (MPN/100 mL of total coliforms and thermotolerant coliforms. All samples indicated presence of total coliforms. Concerning to thermotolerant coliforms, all samples were within the standard. Although the samples did not indicate extensive fecal contamination, it should be emphasized the importance of a rigorous system of sanitary surveillance in irrigation water of vegetable crops, improving the hygienic-sanitary conditions of vegetables offered to residents.

  10. Bactérias coliformes totais e coliformes de origem fecal em águas usadas na dessedentação de animais Total and fecal coliform bacteria in animal drinking water

    Luiz Carlos Souza

    1983-04-01

    Full Text Available Foram colhidas 105 amostras de água sendo 44 de mananciais e de 61 bebedouros, a partir das quais foram realizadas as determinações dos NMP (Número Mais Provável de bactérias coliformes totais e fecais. De acordo com a Portaria GM/0013 de 15 de janeiro de 1976, da Secretaria Especial do Meio Ambiente do Ministério do Interior, os 44 mananciais revelaram-se dentro dos parâmetros estabelecidos. Embora não haja referência com relação a bebedouros, aplicando-se os valores estabelecidos para mananciais, das 61 amostras estudadas, somente 6 (9,8% não poderiam ser usadas para a dessedentação de animais. Das 105 amostras analisadas, verificou-se que as condições sanitárias revelaram-se não satisfatórias em 6 (5,7% amostras de bebedouros quanto a coliformes fecais, sendo 3 (2,8% também com relação a coliformes totais.The objective of the present experiment was to get information on the sanitary quality of water drunk by animals in the Botucatu region, S. Paulo State, Brazil. MPN (More Provable Number counts of coliform and fecal coliform bacteria of 105 water samples were made. Of these samples 44 were taken from fountains and 61 from watering troughs. According to the Brazilian government directive, of the Ministry of the Interior, the 44 fountains were shown to be in accordance with the established parameters. As this government directive does not make reference to watering troughs, applying the same values established for fountains, it was observed that of 61 of the samples examined, only 6 (9.8% proved to be outside the parameters. Of the 105 samples examined, all showed good sanitary conditions, except 6 samples (9.8% of watering troughs in relation to fecal coliforms. Of these 6 samples, 3 showed bad sanitary conditions too, but only regard to total coliforms.

  11. Pengaruh Ketebalan Media dan Rate filtrasi pada Sand Filter dalam Menurunkan Kekeruhan dan Total Coliform

    Deni Maryani

    2014-09-01

    Full Text Available Pada penelitian ini dilakukan proses filtrasi dengan menggunakan sand filter sebagai salah satu metode dalam pengolahan air bersih. Pada sand filter proses penyaringan terjadi pada media filter yang sangat halus, seperti media filter pada unit slow sand filter. Kecepatan penyaringan yang diinginkan pada sand filter ini adalah kecepatan seperti pada unit rapid sand filter. Sehingga sand filter ini adalah penggabungan antara kelebihan yang dimiliki slow sand filter dan rapid sand filter. Variasi pada penelitian ini yaitu: tebal media pasir 80 cm dengan rate filtrasi 5 m3/m2.jam, tebal media pasir 80 cm dengan rate filtrasi 7,5 m3/m2.jam, tebal media pasir 100 cm dengan rate filtrasi 5 m3/m2.jam, tebal media pasir 100 cm dengan rate filtrasi 7,5 m3/m2.jam, tebal media pasir 120 cm dengan rate filtrasi 5 m3/m2.jam dan tebal media pasir 120 cm dengan rate filtrasi 7,5 m3/m2.jam. Pada penelitian ini digunakan air baku yaitu air Kali Surabaya dengan nilai rata-rata total coliform 90.000 per 100 ml sampel dan nilai rata-rata kekeruhan 87,4 NTU. Dihasilkan bahwa penyisihan total coliform pada variasi tebal media 120 cm dan rate filtrasi 5 m3/m2/jam dengan nilai efisiensi sebesar 99% dan kekeruhan paling baik terjadi pada variasi panjang variasi tebal media 100 cm dan rate filtrasi 5 m3/m2/jam dengan nilai efisiensi sebesar 98,27%.

  12. Assessing the sources of high fecal coliform levels at an urban tropical beach

    Aline Mendonça Cavalcante Davino

    2015-01-01

    Full Text Available Recreational water quality is commonly assessed by microbial indicators such as fecal coliforms. Maceió is the capital of Alagoas state, located in tropical northeastern Brazil. Its beaches are considered as the most beautiful urban beaches in the country. Jatiúca Beach in Maceió was found to be unsuitable for bathing continuously during the year of 2011. The same level of contamination was not observed in surrounding beaches. The aim of this study was to initiate the search for the sources of these high coliform levels, so that contamination can be eventually mitigated. We performed a retrospective analysis of historical results of fecal coliform concentrations from 2006 to 2012 at five monitoring stations located in the study region. Results showed that Jatiúca Beach consistently presented the worst quality among the studied beaches. A field survey was conducted to identify existing point and non-point sources of pollution in the area. Monitoring in the vicinity of Jatiúca was spatially intensified. Fecal coliform concentrations were categorized according to tide range and tide stage. A storm drain located in northern Jatiúca was identified as the main point source of the contamination. However, fecal coliform concentrations at Jatiúca were high during high tides and spring tides even when this point source was inactive (no rainfall. We hypothesize that high fecal coliform levels in Jatiúca Beach may also be caused by aquifer contamination or, more likely, from tide washing of contaminated sand. Both of these hypotheses will be further investigated.

  13. Assessment of the Water Quality in Buhisan, Bulacao and Lahug Rivers, Cebu, Philippines Using Fecal and Total Coliform as Indicators

    Eukene Bensig; Mary Joyce flores; Fleurdeliz Maglangit

    2014-01-01

    This study was conducted to evaluate and compare the water quality of Buhisan, Bulacao and Lahug rivers in Cebu, Philippines using coliforms as indicators. Monthly sampling started from November 2011 to April 2012 in the upstream, midstream and downstream stations. The multiple tube fermentation technique was used for the analysis of coli forms. High total and fecal coli form counts were observed in the three rivers from February to April, but the difference in values across time was not sign...

  14. Evaluation of media for simultaneous enumeration of total coliform and Escherichia coli in drinking water supplies by membrane filtration techniques

    WANG Dunling; FIESSEL Wanda

    2008-01-01

    This study evaluated three different dehydrated media for simultaneous detection and enumeration of total coliform (TC) and Escherichia coil in drinking water samples with a standard membrane filtration procedure.The experiment indicated that the differential coliform agar (DCA) medium was the most effective among the tested media in enumerating TC and E.coli,without the need for extensive accompanying confirmation tests.The results for DCA medium were highly reproducible for both TC and E.coli with standard deviation of 6.0 and 6.1,respectively.A high agreement (82%) was found between DCA and m-Endo media on 152 drinking water samples in terms of TC positive.The DCA medium also reduced concealment of background bacteria.

  15. Assessing the sources of high fecal coliform levels at an urban tropical beach

    Aline Mendonça Cavalcante Davino; Milena Bandeira de Melo; Roberto Augusto Caffaro Filho

    2015-01-01

    Recreational water quality is commonly assessed by microbial indicators such as fecal coliforms. Maceió is the capital of Alagoas state, located in tropical northeastern Brazil. Its beaches are considered as the most beautiful urban beaches in the country. Jatiúca Beach in Maceió was found to be unsuitable for bathing continuously during the year of 2011. The same level of contamination was not observed in surrounding beaches. The aim of this study was to initiate the search for the sources o...

  16. 水质污染指示菌--总大肠菌群%Indicator Bacteria of Water Fecal Pollution---The Total Coliform Group

    胡睿娟

    2015-01-01

    ABSTRACT:This paper analyzes the reasons why the total coliform group is regarded as the indicator bacteria of water fecal pollution, compares several methods for detecting the total coliform group, and introduces the significance of the detection of the total coliform group, thermotoletant coliform bacteria and Escherichia coli and the relationship among them.%分析了总大肠菌群作为水质污染指示菌的原因,比较了总大肠菌群的几种检测方法,介绍了总大肠菌群、耐热大肠菌群、大肠埃希氏菌的检测意义以及它们之间的关系。

  17. Assessment of the characteristic of nutrients, total metals, and fecal coliform in Sibu Laut River, Sarawak, Malaysia

    Soo, Chen-Lin; Ling, Teck-Yee; Lee, Nyanti; Apun, Kasing

    2016-03-01

    The concentrations of nutrients (nitrogen and phosphorus), total metals, and fecal coliform (FC) coupling with chlorophyll- a (chl- a), 5-day biochemical oxygen demand (BOD5) and other general environmental parameters were evaluated at the sub-surface and near-bottom water columns of 13 stations in the Sibu Laut River during low and high slack waters. The results indicated that inorganic nitrogen (mainly nitrate) was the primary form of nitrogen whereas organic phosphorus was the major form of phosphorus. The abundance of total heavy metals in Sibu Laut River and its tributaries was in the order of Pb < Cu < Zn < Cd. Fecal coliform concentration was relatively low along Sibu Laut River. The shrimp farm effluents contributed a substantial amount of chl- a, BOD5, nutrients, and FC to the receiving creek except for total metals. Nevertheless, the influence was merely noticeable in the intake creek and amended rapidly along Selang Sibu River and brought minimal effects on the Sibu Laut River. Besides, the domestic sewage effluents from villages nearby also contributed a substantial amount of pollutants.

  18. Matrix Extension Study: Validation of the Compact Dry CF Method for Enumeration of Total Coliform Bacteria in Selected Foods.

    Mizuochi, Shingo; Nelson, Maria; Baylis, Chris; Green, Becky; Jewell, Keith; Monadjemi, Farinaz; Chen, Yi; Salfinger, Yvonne; Fernandez, Maria Cristina

    2016-01-01

    The Compact Dry "Nissui" CF method, Performance Tested Method(SM) 110401, was originally certified for enumeration of coliform bacteria by the AOAC Research Institute Performance Tested Methods(SM) program for raw meat products. Compact Dry CF is a ready-to-use dry media sheet, containing a cold-soluble gelling agent, a chromogenic medium, and selective agents, which are rehydrated by adding 1 mL of diluted sample. Coliform bacteria produce blue/blue-green colonies on the Compact Dry CF, allowing a coliform colony count to be determined in the sample after 24 ± 2 h incubation. A validation study was organized by Campden BRI (formerly Campden and Chorleywood Food Research Association Technology, Ltd), Chipping Campden, United Kingdom, to extend the method's claim to include cooked chicken, fresh bagged prewashed shredded iceberg lettuce, frozen fish, milk powder, and pasteurized 2% milk. Campden BRI collected single-laboratory data for cooked chicken, lettuce, frozen fish, and milk powder, whereas a multilaboratory study was conducted on pasteurized milk. Thirteen laboratories participated in the interlaboratory study. The Compact Dry CF method was compared to ISO 4832:2006 "Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coliforms-Colony-count technique," the current version at the time this study was conducted. Each matrix was evaluated at either four or five contamination levels of coliform bacteria (including an uncontaminated level). After logarithmic transformation of counts at each level, the data for pasteurized whole milk were analyzed for sr, sR, RSDr, and RSDR. Regression analysis was also performed and r(2) was reported. Mean difference between methods with 95% confidence interval (CI) was calculated. A log10 range of -0.5 to 0.5 for the CI was used as the acceptance criterion to establish significant statistical difference between methods. In the single-laboratory evaluation (for cooked chicken, lettuce, frozen

  19. ANÁLISIS DE LA CONTAMINACIÓN MICROBIOLÓGICA (COLIFORMES TOTALES Y FECALES EN LA BAHÍA DE SANTA MARTA, CARIBE COLOMBIANO Analysis Of The Microbiological Contamination (Total And Fecales Coliforms In The Bay Of Santa Marta, Colombian Caribbean

    LINA MARÍA RAMOS-ORTEGA

    Full Text Available Con el fin de realizar un análisis del nivel de contaminación microbiológica y de las principales fuentes de contaminación en la Bahía de Santa Marta (BSM, se colectaron muestras de agua para medir las concentraciones de coliformes totales y fecales en 11 estaciones y dos niveles de profundidad (1 y 20 m. Para la época de mayores precipitaciones se encontraron altos valores de coliformes totales y fecales, en los dos niveles de profundidad; presentándose una condición similar para la época seca. Con base a una escala conceptual se obtuvo que la BSM presenta un grado de contaminación medio para actividades de contacto primario (natación y buceo, sin embargo, existen varias fuentes de contaminación (emisario submarino, río, puerto marítimo, entre otras que están generando un alto impacto sobre este ecosistema. Los altos valores de contaminación que se registran en la BSM se encuentran asociados a la proximidad que tiene esta a las zonas urbanas.With the purpose of making an analysis of the microbiological level of contamination and the main sources of contamination in the Bay of Santa Marta (BSM, water samples were collected to measure the concentrations of total and fecals coliforms in 11 stations and two levels of depth (1 and 20 m. For the time of greater precipitations were stops values of total and fecals coliforms, in both depth levels; appearing a similar condition for the dry time. Based on a conceptual scale it was obtained that the BSM presents an average degree of contamination for activities of primary contact (swimming and diving, nevertheless, exist several sources of contamination (submarine emissary, river, seaport, among others that are generating a high impact on this ecosystem. The high values of contamination that are registered in the BSM find associate to the proximity that has this to the urban zones.

  20. ANÁLISIS DE LA CONTAMINACIÓN MICROBIOLÓGICA (COLIFORMES TOTALES Y FECALES) EN LA BAHÍA DE SANTA MARTA, CARIBE COLOMBIANO

    Saavedra Lina; Vidal Luis; Vilardy Sandra; Ramos Lina Maria

    2008-01-01

    Con el fin de realizar un análisis del nivel de contaminación microbiológica y de las principales fuentes de contaminación en la Bahía de Santa Marta (BSM), se colectaron muestras de agua para medir las concentraciones de coliformes totales y fecales en 11 estaciones y dos niveles de profundidad (1 y 20 m). Para la época de mayores precipitaciones se encontraron altos valores de coliformes totales y fecales, en los dos niveles de profundidad; presentándose una condición similar para la época ...

  1. A high-performance doped photocatalysts for inactivation of total coliforms in superficial waters using different sources of radiation.

    Claro, Elis Marina Turini; Bidoia, Ederio Dino; de Moraes, Peterson Bueno

    2016-07-15

    Photocatalytic water treatment has a currently elevated electricity demand and maintenance costs, but the photocatalytic water treatment may also assist in overcoming the limitations and drawbacks of conventional water treatment processes. Among the Advanced Oxidation Processes, heterogeneous photocatalysis is one of the most widely and efficiently used processes to degrade and/or remove a wide range of polluting compounds. The goal of this work was to find out a highly efficient photocatalytic disinfection process in superficial water with different doped photocatalysts and using three sources of radiation: mercury vapor lamp, solar simulator and UV-A LED. Three doped photocatalysts were prepared, SiZnO, NSiZnO and FNSiZnO. The inactivation efficiency of each synthesized photocatalysts was compared to a TiO2 P25 (Degussa(®)) 0.5 g L(-1) control. Photolysis inactivation efficiency was 85% with UV-A LED, which is considered very high, demanding low electricity consumption in the process, whereas mercury vapor lamp and solar simulator yielded 19% and 13% inactivation efficiency, respectively. The best conditions were found with photocatalysts SiZnO, FNSiZnO and NSiZnO irradiated with UV-A LED, where efficiency exceeded 95% that matched inactivation of coliforms using the same irradiation and photocatalyst TiO2. All photocatalysts showed photocatalytic activity with all three radiation sources able to inactivate total coliforms from river water. The use of UV-A LED as the light source without photocatalyst is very promising, allowing the creation of cost-effective and highly efficient water treatment plants. PMID:27107952

  2. Estimation and modeling of direct rapid sand filtration for total fecal coliform removal from secondary clarifier effluents.

    Li, Yi; Yu, Jingjing; Liu, Zhigang; Ma, Tian

    2012-01-01

    The filtration of fecal coliform from a secondary clarifier effluent was investigated using direct rapid sand filters as tertiary wastewater treatment on a pilot scale. The effect of the flocculation dose, flow loading rate, and grain size on fecal coliform removal was determined. Direct rapid sand filters can remove 0.6-1.5 log-units of fecal coliform, depending on the loading rate and grain size distribution. Meanwhile, the flocculation dose has little effect on coliform removal, and increasing the loading rate and/or grain size decreases the bacteria removal efficiency. A model was then developed for the removal process. Bacteria elimination and inactivation both in the water phase and the sand bed can be described by first-order kinetics. Removal was successfully simulated at different loading rates and grain size distributions and compared with the data obtained using pilot-scale filters. PMID:22508124

  3. Avaliação do padrão coliformes a 45ºC e comparação da eficiência das técnicas dos tubos múltiplos e Petrifilm EC na detecção de coliformes totais e Escherichia coli em alimentos Coliforms at 45ºc and comparison of most probable number method and Petrifilm EC for enumeration of total coliforms and Escherichia coli of foods

    M.P. Silva

    2006-06-01

    coliforms at 45ºC and to compare the methods of Most Probable Number Method (MPN and Petrifilm EC in their efficiency to detect E. coli in Minas cheese, sausage, fresh vegetables and corn flower samples. Petrifilm EC was more accurate method than MNP method, which presented false negative results or underestimation of E. coli. In conclusion, Petrifilm EC was an efficient and practical method to detect E. coli. Therefore, it may be alternatively used for enumeration of total coliforms and E. coli in foods.

  4. Total Coliforms and Turbidity Removal of Water in the Continuous Sand Filter

    AH Mahvi; R Sheikhi; Naddafi, K

    2003-01-01

    The continuous filter is a kind of sand filter, which will operate without any interruptions for backwashing and also it accepts high-suspended solid levels in feed stream. Fouled sand is continuously removed from the filter bed, washed and recycled back without interruption with filtration process. Various samples of water with certain amounts of turbidity enter through a feed pipe and being distributed to the filter. A central column runs from top to bottom of the filter. The water is led t...

  5. Effect of adding mucilage from Opuntia ficus-indica and Opuntia atropes to raw milk on mesophilic aerobic bacteria and total coliforms

    R. Ortiz-Rodríguez; J.L. Aguilar-Barrera; J.J. Valdez-Alarcón; D. Val-Arreola; J Esquivel-Córdoba; H.E. Martínez-Flores; R.E. Pérez-Sánchez

    2016-01-01

    Se evaluó la adición de mucílago líquido y deshidratado de Opuntia ficus-indica y Opuntia atropes a la leche cruda sobre el crecimiento de bacterias mesófilas aerobias y coliformes totales. Las muestras de leche cruda de vacas Holstein de un sistema de producción familiar se adicionaron con mucílago líquido o deshidratado de O. ficus-indica u O. atropes en concentraciones de 0.5, 1.0 y 2.0%. En las muestras se midió el crecimiento de bacterias mesófilas aerobias y coliformes totales. Los resu...

  6. Comparison of methods for enumeration of total coliforms and Escherichia coli in water samples in the Netherlands

    Schets FM; Nobel PJ; Strating S; Mooijman KA; Engels GB; Brouwer A; Kiwa; PWN; MGB

    2001-01-01

    The new European Drinking Water Directive (December 1998) defines a reference method for estimating the concentration of coliforms and Escherichia coli in drinking water. Laboratories may use other methods, but should demonstrate that results obtained are at least as reliable as those produced with

  7. Effect of adding mucilage from Opuntia ficus-indica and Opuntia atropes to raw milk on mesophilic aerobic bacteria and total coliforms

    R. Ortiz-Rodríguez

    2016-01-01

    Full Text Available Se evaluó la adición de mucílago líquido y deshidratado de Opuntia ficus-indica y Opuntia atropes a la leche cruda sobre el crecimiento de bacterias mesófilas aerobias y coliformes totales. Las muestras de leche cruda de vacas Holstein de un sistema de producción familiar se adicionaron con mucílago líquido o deshidratado de O. ficus-indica u O. atropes en concentraciones de 0.5, 1.0 y 2.0%. En las muestras se midió el crecimiento de bacterias mesófilas aerobias y coliformes totales. Los resultados se analizaron mediante los modelos de efectos fijos y las comparaciones entre grupos se realizaron por el método de medias de mínimo cuadrados. En general, las cuentas bacterianas para mesófilas aerobias y coliformes totales disminuyeron (P < 0,05 cuando se adicionó mucilago a la leche cruda al 0,5%, independientemente de la especie o de la forma física (deshidratado o líquido. Se observó disminución de cuentas bacterias para mesófilas aerobias (P < 0.05 cuando se adicionó mucílago deshidratado de O. ficus indica (Log10 2.6 UFC mL-1 y O. atropes (Log10 2,7 UFC mL-1 en concentración de 0.5%, respectivamente. Mientras que el grupo Control (leche cruda sin adición de mucílago contenía Log10 3.6 UFC mL-1 de mesófilas aerobias. La mejor respuesta para coliformes totales se observó con el mucílago deshidratado de O. ficus-indica (Log10 3.0 UFC mL-1 y mucílago líquido de O. atropes (Log10 2,7 UFC mL1, ambos adicionados al 1%. El mucílago deshidratado o líquido de O. ficus-indica y O. atropes reducen el contenido microbiano de la leche cruda.

  8. The Effect of Colony Total and Coliform Bacteria in Bean Paste Stuffing Foresaw of Different Packing%不同包装对豆沙馅料中菌落总数和大肠菌群的影响

    李静; 操庆国

    2012-01-01

    本文针对冷、热真空包装豆沙馅料中菌落总数和大肠菌群进行了测定。试验证明,冷真空包装中菌落和大肠菌群可能超出国家标准,而热真空包装中菌落和大肠菌类不超出国家标准。%This article determined the colony total and coliform bacteria in bean paste stuffing foresaw of cold and hot vacuum packing. The experiment proved that, in the cold vacuum packing colony total and coliform bacteria possibly sur- passes the national standards, but in the hot vacuum packing colony total and coliform bacteria does not surpass the national standards.

  9. Análisis de la contaminación microbiológica (coliformes totales y fecales) en la bahía de santa marta, caribe colombiano

    Ramos, Lina Maria; Vidal, Luis (S.I.); Vilardy, Sandra; Saavedra, Lina

    2010-01-01

    Con el fin de realizar un análisis del nivel de contaminación microbiológica y de las principales fuentes de contaminación en la Bahía de Santa Marta (BSM), se colectaron muestras de agua para medir las concentraciones de coliformes totales y fecales en 11 estaciones y dos niveles de profundidad (1 y 20 m). Para la época de mayores precipitaciones se encontraron altos valores de coliformes totales y fecales, en los dos niveles de profundidad; presentándose una condición similar para la época ...

  10. 40 CFR 141.21 - Coliform sampling.

    2010-07-01

    ... carefully curl and insert the membrane into a tube of EC medium (the laboratory may first remove a small... total coliform-positive, the system must analyze that total coliform-positive culture medium to... business day. (2) The State has the discretion to allow a public water system, on a case-by-case basis,...

  11. Full-scale studies of factors related to coliform regrowth in drinking water.

    LeChevallier, M W; Welch, N J; Smith, D B

    1996-07-01

    An 18-month survey of 31 water systems in North America was conducted to determine the factors that contribute to the occurrence of coliform bacteria in drinking water. The survey included analysis of assimilable organic carbon (AOC), coliforms, disinfectant residuals, and operational parameters. Coliform bacteria were detected in 27.8% of the 2-week sampling periods and were associated with the following factors: filtration, temperature, disinfectant type and disinfectant level, AOC level, corrosion control, and operational characteristics. Four systems in the study that used unfiltered surface water accounted for 26.6% of the total number of bacterial samples collected but 64.3% (1,013 of 1,576) of the positive coliform samples. The occurrence of coliform bacteria was significantly higher when water temperatures were > 15 degrees C. For filtered systems that used free chlorine, 0.97% of 33,196 samples contained coliform bacteria, while 0.51% of 35,159 samples from chloraminated systems contained coliform bacteria. The average density of coliform bacteria was 35 times higher in free-chlorinated systems than in chloraminated water (0.60 CFU/100 ml for free-chlorinated water compared with 0.017 CFU/100 ml for chloraminated water). Systems that maintained dead-end free chlorine levels of systems that maintained higher disinfectant residuals. Free-chlorinated systems with AOC levels greater than 100 micrograms/liter had 82% more coliform-positive samples and 19 times higher coliform levels than free-chlorinated systems with average AOC levels less than 99 micrograms/liter. Systems that maintained a phosphate-based corrosion inhibitor and limited the amount of unlined cast iron pipe had fewer coliform bacteria. Several operational characteristics of the treatment process or the distribution system were also associated with increased rates of coliform occurrence. The study concludes that the occurrence of coliform bacteria within a distribution system is dependent upon

  12. 总大肠菌群检测试剂盒在生活饮用水检测中的应用评价%Application Evaluation of Total Coliforms Group Detection Kit for Drinking Water

    赵娣伟

    2011-01-01

    目的 评价总大肠菌群检测试剂盒应用于生活饮用水中总大肠菌群检测的准确性和价值.方法 对124份生活饮用水水样,分别用总大肠菌群检测试剂盒法与国家标准认可的多管发酵法同时进行总大肠菌群检测,采用流行病学方法对检测结果进行准确性评价.结果 以国标中的检测方法作为金标准比较,总大肠菌群检测试剂盒法的灵敏度为100%,特异度为98.18%,正确指数为98.18%.结论 总大肠菌群检测试剂盒法具有很好的准确性,而且检测省时、省力,简便易行,适用于大批量样品快速检测,特别是突发事件时检验工作的开展.%[Objective]To evaluate the accuracy and value of total coliforms group detection kit for testing the total coliforms group in drinking water.[Methods]124 drinking water samples were tested for the total coliforms group by total coliforms group detection kit and multiple-tube fermentation which is the national standard method, simultaneously. The accuracy of test results was evaluated by epidemiologjcal methods.[Results]Using the national standard method as the gold standard, the sensitivity, specificity and correct index of total coliforms group detection kit was 100% , 98. 18% and 98. 18% , respectively.[Conclusion]The total coliforms group detection kit has good accuracy, which is simple, convenient and time saving. It is suitable for rapid detection of large numbers of samples, especially in detection of emergent events.

  13. Improvement of Sealing Technology of Enzyme Substrate Method in Measuring the Total Coliforms/Fecal coliforms%总大肠菌群/粪大肠菌群检测方法中酶底物法封口技术的改进

    李宗全; 黄芳; 许江

    2014-01-01

    Experiments on replacing the special program-controlled sealing machine by the household electric iron were conducted for the enzyme substrate method in measuring the total coliforms and fecal coliforms.The experi-mental results showed that the electric iron was much convenient with low cost.%通过试验,验证了在科立得固定底物技术酶底物法检测大肠菌群/粪大肠菌群的方法中,可使用家用电熨斗替换专用程控定量封口机进行封口,且经济、方便。

  14. RAPID MONITORING OF INDICATOR COLIFORMS IN DRINKING WATER BY AN ENZYMATIC ASSAY

    M. Nikaeen ، A. Pejhan ، M. Jalali

    2009-01-01

    Full Text Available Coliform group has been extensively used as an indicator of drinking water quality and historically led to the public health protection concept. Multiple tube fermentation technique has been currently used for assessment of the microbial quality of drinking water. This method, however, has limitations. Enzymatic assay constitute an alternative approach for detecting indicator bacteria, namely total coliforms and E.coli in various aquatic environments. This study compared the performance of LMX® broth as an enzymatic assay with the standard methods multiple tube fermentation technique and presence–absence test, for the detection of indicator coliforms in drinking water samples. In addition, the potential effect of water quality on the microbial detection method was assayed through measurement of some physicochemical parameters. From the 50 drinking water samples tested, 8 (16% and 7 (14% contained total coliforms and E.coli as indicated by all three techniques. Although on average the LMX recovered more total coliforms and E.Coli numbers comparing to multiple tube fermentation, but there was no significant difference. A significant difference existed between the level of residual chlorine for positive and negative samples. In conclusion, enzymatic assay showed a rapid and less labor method, allowing the simultaneous detection of total coliforms and E.coli. The method is particularly useful in the early warning of fecal pollution of drinking water.

  15. Fecal coliform modeling under two flow scenarios in St. Louis Bay of Mississippi.

    Liu, Zhijun; Hashim, Noor B; Kingery, William L; Huddleston, David H

    2010-01-01

    St. Louis Bay, along with its two major tributaries, Wolf River and Jourdan River, are included in the Mississippi 1998 Section 303(d) List for violation of the designated water use of recreation and shellfish harvesting. Fecal coliform was identified as one of the pollutants that caused the water quality impairment. In order to facilitate the total maximum daily loads (TMDL) development, the fecal coliform dynamics was investigated under 2 flow scenarios with a calibrated and validated modeling framework by integration of Environmental Fluid Dynamic Code (EFDC) and Hydrological Simulation Program Fortran (HSPF). EFDC was used to model the hydrodymics and fecal coliform transportation in the Bay and the tributaries, whereas HSPF was applied to compute the flow and fecal coliform loadings from the watersheds. The total amount of precipitation in the dry year simulation corresponds to a 50-year return period of low flow condition, and a 10-year return period of high flow condition for wet weather simulation. For EFDC modeling, the fecal coliform sources considered were the contributions from the 2 upper watersheds (no tidal influence), the 28 small surrounding watershed, and 12 municipal, industrial, and domestic point sources. When simulating the fecal coliform loadings from the 2 upper watersheds using HSPF, the simulated non-point source loadings of fecal coliform included wildlife, land application of hog and cattle manure, land application of poultry litter, and grazing animals. The EFDC modeling results indicated that the wet weather exerted greater stress on fecal coliform water quality conditions. The number of exceedance of fecal coliform water quality standard in wet year simulation is much higher than that in dry year simulation. The impact of the upper rural watersheds loads on fecal coliform levels in the St. Louis Bay is much less significant than that from the surrounding urban runoff. Fecal coliform TMDL development should be based on high flow

  16. Detection of Total Bacteria and Coliform in Drinking Water Resources of Cangshan Huadianba in Dali%大理苍山花甸坝饮用水源细菌总数和大肠菌群的检测

    张发; 周娅; 和东义; 李小艳; 杨晓燕

    2013-01-01

    Objective:To detect the total count of bacteria and coliform bacteria of the three drinking water resources of Longtan, Xipo and Farm headquarters of Cangshan Huadianba. Methods:Using the methods of plate culture count and multiple-tube fermentation technique to detect the total count of bacteria and coliform bacteria in the three drinking water sources respectively. Results:The bac-teria and coliform bacteria were not detected in the water resource of Longtan, but a large number of bacteria and coliform bacteria were detected in the drinking water resources of Xipo and Farm headquarters. The total count of bacteria and coliform bacteria was more than 300/mL and 230/L respectively in the drinking water resources of Xipo. It was 189/mL and 230/L respectively in the drinking wa-ter resources of the Farm headquarters. Conclusion:The water drinking resource in Longtan meets the requirements of current nation-al standards for drink water (GB5749-2006), while two other drinking resources do not meet this standard.%目的:检测苍山花甸坝龙潭、西坡、农场总部3个饮用水源中的细菌总数和大肠菌群。方法:采用平板菌落计数法和多管发酵法分别对水样中的细菌总数和大肠菌群进行检测。结果:龙潭水源中未检到细菌和大肠菌群,而农场总部水源和西坡水源中检出大量细菌和大肠菌群,其中西坡水源中细菌总数>300/mL,大肠菌群数>230/L;农场总部水源细菌总数=189/mL,大肠菌群数=230/L。结论:苍山花甸坝农场总部水源和西坡水源中的细菌总数和大肠菌群均不符合我国生活饮用水卫生标准(GB5749-2006),不适于直接饮用,而龙潭水源则符合该标准。

  17. Detecting of Total Bacteria and Coliform in Rural Drinking Water Resources at Guangnan of Wenshan%文山州广南县农村饮用水源细菌总数和大肠菌群的检测

    周建芬; 张发; 林慧彪; 范先芹; 杨晓燕

    2014-01-01

    The sanitary condition of drinking water in rural areas of Wenshan Yunnan province is investigated through testing total bacteria and coliform bacteria contained in nine representative drinking water resources in Guangnan county of Wenshan Prefecture. Total bacteria and coliform bacteria in the water samples, collected from nine water resources, are tested using plate count methods and multiple-tube fermentation technique. The total bacteria is less than 100/ml in No. 6 and 7 water samples, but the rest are more than 100/ml. Coliform bacteria have been detected in all samples. The water quality from nine water resources does not meet drinking water standards(GB5749-2006), so it is unift to drink directly.%对文山州广南县9个具有代表性的农村饮用水源点进行细菌总数和大肠菌群的检测,初步了解云南省文山州广南县农村饮用水卫生状况。在选定的9个水源点进行水样采集,并用平板菌落计数法和多管发酵法分别对水样中细菌总数和大肠菌群进行检测。发现水样6和水样7细菌总数100个/mL;9个水样的大肠菌群均有检出。通过检测发现,所抽取9个饮用水水源均不符合我国饮用水卫生标准(GB5749-2006),不适宜直接饮用。

  18. Nutrient and Coliform Loading (NCL)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is a database of available fecal coliform bacteria, fecal streptococci bacteria, and nutrient loading data. Loading for contaminants other than fecal coliform...

  19. Arsenic presence and coliforms in drinking water of the Tecuala municipality, Nayarit, Mexico; Presencia de arsenico y coliformes en agua potable del municipio de Tecuala, Nayarit, Mexico

    Mora B, D.; Gonzalez A, C. A.; Medina D, I. M.; Robledo M, M. L.; Rojas G, A. E. [Universidad Autonoma de Nayarit, Secretaria de Investigacion y Posgrado, Ciudad de la Cultura Amado Nervo, 63155 Tepic, Nayarit (Mexico); Sanchez P, L. C.; Del Razo, L. M., E-mail: aerg81@gmail.com [IPN, Centro de Investigacion y de Estudios Avanzados, Departamento de Toxicologia, Av. IPN No. 2508, Col. San Pedro Zacatenco, 07360 Mexico D. F. (Mexico)

    2012-07-01

    Total arsenic concentrations (t As) and the presence of total and fecal coliforms in drinking water from Tecuala, Nayarit, Mexico, were determined. The presence of t As was analyzed by means of hydride generation atomic fluorescence spectrometry. Total and fecal coliforms were determined through the Most Probable Number (MPN) method. According to the results, the mean concentration of t As in Tecuala wa 15.82 {mu}g/L, in Atotonilco 19.88 {mu}g/L, in Pajaritos 21.49 {mu}g/L, in Quimichis 17.80 {mu}g/L, and in Playas de Novillero 19.79 {mu}g/L. The t As concentrations in drinking water from Tecuala, Nayarit, are within the limit set by the Mexican official standards (25 {mu}g/L); still, they are over the limit established by the World Health Organization (10 {mu}g/L). The concentration of coliforms in water from wells 1 and 3 was 180 MPN/100 ml and 43 MPN/100 ml for well water 2. The presence of total and fecal coliforms, suggest the infiltration of sewage which could increase the levels of dissolved arsenic. The results of this study will serve as an antecedent of the water quality in Tecuala, Nayarit. (Author)

  20. Arsenic presence and coliforms in drinking water of the Tecuala municipality, Nayarit, Mexico

    Total arsenic concentrations (t As) and the presence of total and fecal coliforms in drinking water from Tecuala, Nayarit, Mexico, were determined. The presence of t As was analyzed by means of hydride generation atomic fluorescence spectrometry. Total and fecal coliforms were determined through the Most Probable Number (MPN) method. According to the results, the mean concentration of t As in Tecuala wa 15.82 μg/L, in Atotonilco 19.88 μg/L, in Pajaritos 21.49 μg/L, in Quimichis 17.80 μg/L, and in Playas de Novillero 19.79 μg/L. The t As concentrations in drinking water from Tecuala, Nayarit, are within the limit set by the Mexican official standards (25 μg/L); still, they are over the limit established by the World Health Organization (10 μg/L). The concentration of coliforms in water from wells 1 and 3 was 180 MPN/100 ml and 43 MPN/100 ml for well water 2. The presence of total and fecal coliforms, suggest the infiltration of sewage which could increase the levels of dissolved arsenic. The results of this study will serve as an antecedent of the water quality in Tecuala, Nayarit. (Author)

  1. Effects of UV light disinfection on antibiotic-resistant coliforms in wastewater effluents

    Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform population resistant to tetracycline or chloramphenicol was significantly higher than the percentage of the total coliform population resistant to those antibiotics before UV irradiation. This finding was attributed to the mechanism of R-factor mediated resistance to tetracycline. No significant difference was noted for the percentage of the surviving total coliform population resistant to streptomycin before or after UV irradiation. Multiple drug resistant to patterns of 300 total coliform isolates revealed that 82% were resistant to two or more antibiotics. Furthermore, 46% of these isolates were capable of transferring antibiotic resistance to a sensitive strain of Escherichia coli

  2. FECAL COLIFORM INCREASE AFTER CENTRIFUGATION

    The Water Environment Research Foundation (WERF) recently published a report titled Examination of Reactivation and Regrowth of Fecal Coliforms in Anaerobically Digested Sludges. Seven full-scale publicly owned treatment facilities were sampled several times to determine if bacte...

  3. CCA培养基同时检测水中总大肠菌群和大肠埃希氏杆菌%Chromocult Coliforms Agar (CCA) Medium for the Simultaneous Detection of Total Coliforms and E.coli in Water

    刘路

    2004-01-01

    大肠菌群(Coliforms)和大肠埃希氏杆菌(E. coli)都是判断水体是否存在粪便污染的重要微生物指标.本文介绍了一种可在24 h之内同时检测大肠菌群和大肠埃希氏杆菌数量的CCA培养基.

  4. Full-scale studies of factors related to coliform regrowth in drinking water.

    LeChevallier, M W; Welch, N J; Smith, D. B.

    1996-01-01

    An 18-month survey of 31 water systems in North America was conducted to determine the factors that contribute to the occurrence of coliform bacteria in drinking water. The survey included analysis of assimilable organic carbon (AOC), coliforms, disinfectant residuals, and operational parameters. Coliform bacteria were detected in 27.8% of the 2-week sampling periods and were associated with the following factors: filtration, temperature, disinfectant type and disinfectant level, AOC level, c...

  5. Estimación de la Incertidumbre en los Ensayos Microbiológicos de Coliformes Totales y Fecales en Aguas Y Aguas Residuales Mediante la Técnica de Tubos Múltiples de Fermentación.

    Marlen Robert Pullés

    2005-01-01

    Full Text Available En la microbiología ambiental es de vital importancia contar con un control de la calidad, dentro del cual, debe considerarse el método de ensayo para garantizar una óptima calidad de los datos resultantes. El cálculo de la incertidumbre está siendo abordado por la comunidad de laboratorios y se espera que en un futuro se disponga de directrices más claras en este campo. La incertidumbre, como un atributo cuantificable, es un concepto relativamente nuevo en la historia de las mediciones, a pesar de que los conceptos de error y análisis de error han sido parte de la práctica de la ciencia de la medición por largo tiempo. De manera general se acepta que cuando todas las componentes, conocidas o supuestas, del error han sido evaluadas y se han aplicado las correcciones, aún persiste una incertidumbre acerca de la confiabilidad del resultado expresado, esto es, persiste una duda acerca de qué tan bien representa el resultado de la medida, al valor de la cantidad que está siendo medida. Actualmente existe en nuestro país la problemática derivada de la falta de la información fundamental acerca de los procedimientos para la estimación de la incertidumbre. En este trabajo se realiza una estimación de la incertidumbre en los ensayos microbiológicos de coliformes totales y fecales en aguas y aguas residuales mediante la técnica de Tubos Múltiples de Fermentación, siguiendo las recomendaciones que aparecen en documentos normalizativos nacionales e internacionales. Se ofrecen los resultados alcanzados en la cuantificación de este importante parámetro. Los componentes de la incertidumbre se organizaron en tablas, lo que permite realizar de una forma rápida una apreciación global.

  6. Distribution of coliform bacteria in waste water

    Dau Lal Bohra

    2012-02-01

    Full Text Available Biological activity of water can be apparently judged by the colonization of bacteria (microbes. In order to find out the extent of pollution and the relationship between inorganic matters and microbiota, a quantitative and qualitative analysis of bacteria in various types of sewage waters, namely sewage water by the residential colonies (group I, industrial waste water (group II, sewage treatment hub (group III, unorganized collected waste water (group IV and old residential waste collection center (group V, of Bikaner city (Rajasthan, India was carried out from February, 2010 to May, 2010. Water samples were taken from surface only owing to low depth and investigated for various abiotic factors (viz. transparency, pH, carbonate, bicarbonate, total alkalinity, total hardness, salinity, chloride, calcium, magnesium, sulphate, nitrate, silica, and inorganic phosphorous and biotic factors (viz. number and diversity of bacteria. The domestic sewage water causes major water borne diseases basing upon Total Bacterial Count (TBC and coliform Count (CC. The coliform count in the present study ranged from 2.5 to 5.12 MPN/mL. Comparision of microbial population in sewage water from all different Groups was done and the higher values of TBC and CC were recorded only in Sewage treatement hub (Group III.

  7. Resistência antimicrobiana de coliformes isolados de leite humano ordenhado Antimicrobial resistance of coliform isolates from expressed human milk

    Franz Reis Novak

    2001-06-01

    Full Text Available A veiculação de microrganismos potencialmente patogênicos, resistentes aos antibióticos, por meio de leite humano ordenhado, pode ser um fator de risco potencial. Este estudo teve como objetivo contribuir para um melhor conhecimento dos coliformes isolados de leite humano ordenhado (LHO e de seu perfil de resistência a antimicrobianos. Seguiu-se um delineamento inteiramente casualizado, utilizando 837 amostras de LHO, nas quais 71 (8,48% estavam contaminadas com coliformes totais, cuja contagem em nenhuma das amostras ultrapassava 1,0x10³NMP/ml. A maioria dos microrganismos isolados (91,6% pertencia a apenas duas espécies, Enterobacter cloacae e Klebsiella pneumoniae. As 71 cepas de coliformes apresentavam resistência a pelo menos um dos antimicrobianos testados. Conclui-se que o LHO coletado e armazenado sob condições higiênico-sanitárias insatisfatórias, pode apresentar coliformes, que desgastam os fatores de proteção, reduzem o valor nutricional e a qualidade do produto.The dispersion of potentially pathogenic, antibiotic-resistant microorganisms via expressed human milk can be considered a risk factor. The aim of this study was to contribute to a better understanding of coliform isolates from expressed human milk and their antimicrobial resistance profiles. The sampling scheme followed a totally randomized design, using 837 samples of expressed human milk. Of these, 71 (8.48% were identified as contaminated with total coliforms, although in none of the samples did the population exceed 1.0x10³ MPN/ml. Most of the microorganisms isolated (91.6% belonged to only two species, Enterobacter cloacae and Klebsiella pneumoniae, which when subjected to antibiograms, revealed that several strains showed prior resistance to some of the antimicrobials tested. Coliforms may grow in expressed human milk if it is improperly stored, depleting protection factors and reducing the milk's nutritional value.

  8. THERMOTOLERANT NON-FECAL SOURCE 'KLEBSIELLA PNEUMONIAE': VALIDITY OF THE FECAL COLIFORM TEST IN RECREATIONAL WATERS

    Wisconsin pulp and paper mill processing plants were evaluated for fecal coliform and total Klebsiella (i.e., thermotolerant and thermointolerant) bacterial concentrations. Using the standard fecal coliform test, up to 90 per cent of non-fecal source thermo-tolerant K. pneumoniae...

  9. Asellus aquaticus as a potential carrier of Escherichia coli and other coliform bacteria into drinking water distribution systems

    Christensen, Sarah Christine Boesgaard; Arvin, Erik; Nissen, E.;

    2013-01-01

    . aquaticus transporting bacteria into distribution systems was assessed. Concn. of ≤2 Escherichia coli and 5 total coliforms ml-1 were measured in the water and 200 E. coli and >240 total coliforms ml-1 in the sediments of the investigated ponds. Concn. of A. aquaticus associated bacteria never exceeded 3 E....... coli and 6 total coliforms A. aquaticus-1. During exposure to high concn. of coliforms, concn. reached 350 coliforms A. aquaticus-1. A. aquaticus associated E. coli were only detected as long as E. coli were present in the water and sediment. The calculated probability of exceeding drinking water...... guideline values in non-disinfected systems by intrusion of A. aquaticus was low. Only in scenarios with narrow pipes and low flows, did total coliforms exceed guideline values, implying that the probability of detection by routine monitoring is also low. The study may expand the knowledge base for...

  10. Study on the total colony of bacteria and coliform group during the production process of sausage%红肠不同生产环节中菌落总数和大肠菌群数量变化规律初步研究

    马春华; 陶荣

    2013-01-01

      目的研究红肠不同生产环节、不同环境中菌落总数和大肠菌群数的变化规律。方法用国标方法检测红肠各个工序不同环境中的菌落总数和大肠菌群数,并用统计学软件 SSPS 处理结果。结果建立菌落总数和大肠菌群数量变化规律模型。红肠加工不同环境中菌落总数变化规律的非线性拟合方程为0.4822 y 1768.952=e-x+27.6370.856 x+;24.365大肠菌群数变化规律的非线性拟合方程为2 y=-+x 20.9900.968 x+结论本研究对于控制红肠中微生物数量,保证其安全卫生具有重要意义。。%Objective To study the changes of total colony of bacteria and coliform group during the production process of sausage and different environment. Methods The number of total colony of bacteria and coliform group was analysed using the method of national standard, and the data was processed with SPSS software. Results The nonlinear curves about the number of total colony of bacteria and coliform group were 2 y 1 768.952=e-0.482 x+27.637 0.856 x+24.365;and 2 y= - +x 2 , respectively. Conclusion This study 0.990 0.968 x+is important to control the number of total colony of bacteria and coliform group and guarantee safety and sa-nitation of sausage.

  11. Coliform Bacteria Monitoring in Fish Systems: Current Practices in Public Aquaria.

    Culpepper, Erin E; Clayton, Leigh A; Hadfield, Catherine A; Arnold, Jill E; Bourbon, Holly M

    2016-06-01

    Public aquaria evaluate coliform indicator bacteria levels in fish systems, but the purpose of testing, testing methods, and management responses are not standardized, unlike with the coliform bacteria testing for marine mammal enclosures required by the U.S. Department of Agriculture. An online survey was sent to selected aquaria to document current testing and management practices in fish systems without marine mammals. The information collected included indicator bacteria species, the size and type of systems monitored, the primary purpose of testing, sampling frequency, test methods, the criteria for interpreting results, corrective actions, and management changes to limit human exposure. Of the 25 institutions to which surveys were sent, 19 (76%) responded. Fourteen reported testing for fecal indicator bacteria in fish systems. The most commonly tested indicator species were total (86%) and fecal (79%) coliform bacteria, which were detected by means of the membrane filtration method (64%). Multiple types and sizes of systems were tested, and the guidelines for testing and corrective actions were highly variable. Only three institutions performed additional tests to confirm the identification of indicator organisms. The results from this study can be used to compare bacterial monitoring practices and protocols in fish systems, as an aid to discussions relating to the accuracy and reliability of test results, and to help implement appropriate management responses. Received August 23, 2015; accepted December 29, 2015. PMID:27168340

  12. 生活饮用水中总大肠菌群分离株消毒剂耐药基因检测%Detection of disinfectants-resistant gene qacE△1 in total coliforms isolates from drinking water

    宋红梅; 金玉怀; 李丽婕; 范尉尉; 徐保红; 李丽萍

    2013-01-01

    目的 了解生活饮用水中总大肠菌群分离株耐消毒剂基因qacE△1的存在情况.方法 采用聚合酶链反应(PCR)技术检测102株总大肠菌群分离株(来自2012年1-10月石家庄市城乡生活饮用水总大肠菌群阳性水样)的qacE△1基因,并进行DNA测序.结果 22株大肠埃希菌、27株阴沟肠杆菌、20株柠檬酸杆菌的qacE△1基因的阳性率分别为45.5%,7.4%和10.0%,27株克雷伯菌属细菌、6株产气肠杆菌及1株大肠杆菌标准株均未检出qacE△l基因.结论 生活饮用水中总大肠菌群分离株已携带消毒剂耐药基因,其中,大肠埃希菌携带率较高.%Objective To investigate the disinfectants-resistant gene qacE△l in total coliforms isolates from drinking water.Methods The disinfectants-resistant gene of qacE△l was analyzed by polymerase chain reaction(PCR) and verified by DNA sequencing for 102 coliforms isolates from total coliform-positive drinking water samples of Shijiazhuang urban and rural areas between January and October,2012.Results The positive rates of qacE△1 gene in 22 strains of Escherichia coli,27 strains of Enterobacter cloacae,20 strains of Citrobacter spp.were 45.5%,7.4% and 10.0% respectively.But in 27 strains of Klebsiella spp.,6 strains of Enterobacter aerogenes and one standard control strain,the qacE△1 gene was not detected.Conclusion The total coliforms isolates from drinking water have carried disinfectansts-resistant gene.The higher carrying rate was found in E.coli.

  13. Early Serum Procalcitonin Level after Primary Total Hip Replacement

    Samy Bouaicha

    2013-01-01

    Full Text Available Background. Procalcitonin (PCT is a useful surrogate marker for the differentiation of postoperative infection and unspecific inflammatory reaction after surgery. It is known that postoperative course of the PCT serum level varies with type of surgery. No data exists about the postoperative course of serum PCT levels after primary total hip replacement (THR. Purpose. To characterize early postoperative serum PCT levels in uneventful primary THR compared to postoperative levels of different frequently used inflammatory blood parameters. Method. We prospectively investigated 31 patients. Blood samples were taken preoperatively and for 5 days postoperatively. PCT levels were compared with C-reactive protein (CRP, interleukin-6 (IL-6, and blood leucocyte counts (WBC. Results. In uneventful THR PCT levels showed a uniform low-level course with a peak at the second postoperative day. At the fifth day values returned to almost preoperative levels. On contrary, CRP levels remained high during the entire observational period. Only IL-6 levels showed a peak at postoperative day one with a quick and uniform return to preoperative levels. Conclusion. Similar to observations in cardiothoracic, intestinal, and neural surgeries, postoperative course of PCT after primary THR showed a uniform low-level course with a peak at the second postoperative day but below expected levels in systemic infections.

  14. Remoção de matéria orgânica, coliformes totais e nitrificação no tratamento de esgotos domésticos por filtros de areia Organic matter and total coliform removal and nitrification in the treatment of domestic wastewater by sand filter

    Adriano Luiz Tonetti

    2005-09-01

    Full Text Available Segundo o IBGE (2000, 73% dos municípios brasileiros possuem menos de 20.000 habitantes, que em sua maior parte vive na zona rural ou em pequenos núcleos urbanos. Quase a totalidade dos esgotos destas cidades é lançada nos corpos hídricos, contribuindo com o agravamento dos problemas de saúde pública e ambiental. Esses danos podem ser minimizados com o emprego de sistemas de tratamento simples, eficientes, sustentáveis e economicamente viáveis. O filtro anaeróbio com recheio de bambu combinado com filtros de areia satisfaria estas exigências, propiciando um efluente adequado para disposição nos cursos d'água ou para reúso, resguardando as fontes de água potável. A aplicação piloto deste método demonstrou que no emprego de baixas cargas de efluente anaeróbio nos filtros de areia havia uma remoção superior a 96% da DBO, adequação na emissão de coliformes totais e uma completa nitrificação. Aumentando-se as cargas, ocorria uma pequena redução da eficiência, no entanto os resultados ainda eram satisfatórios.According to IBGE (2000 73% of the Brazilian cities are considered small communities with population smaller than 20,000 inhabitants. The majority of domestic wastewater of these places is normally disposed in rivers with no treatment. As a consequence, the irregular disposal may cause health and environmental problems. To reduce this risk it is necessary to develop treatment systems that are simple, efficient, sustainable and of low cost. The treatment of wastewater using up flow anaerobic filter combined with sand filters would be able to satisfy these exigencies, producing an effluent that could be disposed in rivers or even be reused, safeguarding the sources of potable water. The system achieved BOD removal of 96% and complete nitrification for the application of low loads. The increase of the applied loads caused a reduction in the efficiency; however the final values were still satisfactory.

  15. Testing of higher levels of automatisation of total stations

    Spreicer, Marko

    2013-01-01

    The theme of this final thesis is testing higher levels of automation of universal total station, where we tested the Leica TCRA1102plus. This paper discusses the development of surveying and their breakdown. Lists and describes the systems, which enabled a higher degree of automation in modern surveying and facilitate the operator's work in the field. In the practical part of the thesis we tested automation system of tachymeter Leica TCRA1102plus namely, we compared the accuracy of the a...

  16. Review of Risk Status of Groundwater Supply Wells by Tracing the Source of Coliform Contamination

    Nara Somaratne

    2015-07-01

    Full Text Available Coliform source tracking was undertaken on 48 water sources of which 42 are potable in 26 water supply systems spread across South Australia. The water sources in the study vary from unprotected springs in creek beds to deep confined aquifers. The frequency analysis of historical coliform detections indicate that aquifer types, depth to water and casing depth are important considerations; whilst maintaining well integrity and the presence of low permeable clay layers above the production zone are the dominant parameters for minimizing coliform contamination of water supply wells. However, in karst and fractured rock aquifers, pathways for coliform transport exist, as evidenced in the >200 MPN/100 mL level of coliform detection. Data indicate that there is no compelling evidence to support the contention that the wells identified as low risk are contaminated through geological strata and clay barriers. However, data strongly supports the suggestion that coliform detection from sample taps and wellheads stem from the surrounding groundwater and soil-plant sources as a result of failed well integrity, or potentially from coliform bacteria that can persist within biofilms formed on well casings, screens, pump columns and pumps. Coliform sub-typing results show that most coliform bacteria detected in town water supply wells are associated with the soil-water-plant system and are ubiquitous in the environment: Citrobacter spp. (65%, Enterobacter spp. (63%, Pantoea spp. (17%, Serratia spp. (19%, Klebsiella spp. (34%, and Pseudomonas spp. (10%. Overall, 70% of wells harbor detectable thermotolerant coliforms (TTC with potentially 36% of species of animal origin, including Escherichia coli species found in 12% of wells.

  17. Total and free thyroid hormone levels in chronic renal failure.

    Mehta H

    1991-04-01

    Full Text Available The levels of serum total thyroxine (TT4, triiodothyronine (TT3, free T3, (FT3 free T4 (FT4 and thyrotropin (TSH were measured in 127 clinically euthyroid patients with varying grades of chronic renal failure (CRF; and 97 healthy individuals. They were grouped as: Group I containing 93 patients on conservative management; Group II containing 34 patients on regular dialysis therapy; and Group III (normals. Group I patients showed significant decrease in TT3, TT4 and FT3 levels (p less than 0.001 as compared to Group III, whereas FT4 and TSH values in group I were not significantly altered. TT3, TT4 and FT3 levels reduced as the severity of renal damage increased. Variations in TT3, TT4, FT3, FT4 and TSH levels in Group II patients were similar to those in Group I, except for a decrease in TSH levels (p less than 0.05 as compared to normals. Several thyroid function tests are abnormal in CRF patients, however, finding of normal FT4 and TSH levels would indicate functional euthyroid status.

  18. Exposures influencing total IgA level in colostrum.

    Munblit, D; Sheth, S; Abrol, P; Treneva, M; Peroni, D G; Chow, L-Y; Boner, A L; Pampura, A; Warner, J O; Boyle, R J

    2016-02-01

    Immunoglobulin A (IgA) is a predominant immunoglobulin present in human breast milk and is known to play an important role in infant gut immunity maturation. Breast milk composition varies between populations, but the environmental and maternal factors responsible for these variations are still unclear. We examined the relationship between different exposures and levels of IgA in colostrum. The objective of this study was to examine whether exposures analysed influence levels of IgA in colostrum. The present study used 294 colostrum samples from the MecMilk International cohort, collected from women residing in London, Moscow and Verona. Samples were analysed in automated Abbott Architect Analyser. We found an inverse correlation between time postpartum and colostrum total IgA level (r=-0.49, Pcolostrum collection time (Pcolostrum drops rapidly after birth and future studies should always consider this factor in analysis. IgA concentration varied significantly between countries, with the highest level detected in Moscow and lowest in Verona. Mode of delivery effect should be confirmed on larger cohorts. Further work is needed to determine ways to correct for IgA decline over time in colostrum, and to find the cause of variations in IgA levels between the countries. PMID:26387688

  19. Influencing factors in the occurrence of injured coliforms in the drinking water distribution system in the city of La Plata, Argentina.

    Córdoba, María Alejandra; Del Coco, Valeria Fernanda; Minvielle, Marta Cecilia; Basualdo, Juan Angel

    2010-06-01

    The objective of this study was to evaluate the influencing factors in the occurrence of coliforms in the drinking water in La Plata (Argentina) from June 1999 to June 2001. A total of 180 samples were collected from Rio de La Plata (102 samples) and Puelche Aquifer (78 samples); 45 samples were collected for each of the four seasons. The membrane filter procedure was used for isolating bacteria, and each sample was tested for chlorine and pH. The highest percentage of samples positive for coliforms in the two tested media was obtained in summer while the highest percentage of negative samples was obtained in winter. No Escherichia coli was isolated. The percentage of injured coliforms fluctuated between 70 and 100%. The most frequently isolated bacteria was Enterobacter cloacae in summer, Enterobacter agglomerans in autumn and Klebsiella oxytoca in winter and spring. Significant correlations were observed between coliforms and the distance from the initial treatment point, and with the level of free chlorine. We conclude that drinking water contamination in La Plata occurs in the distribution system due to increased temperatures and reduced disinfectant levels, which result in bacterial regrowth. PMID:20154384

  20. FECAL COLIFORM INCREASE AFTER CENTRIFUGATION: EPA PERSPECTIVE

    The Water Environment Research Foundation (WERF) recently published a report titled Examination of Reactivation and Regrowth of Fecal Coliforms in Anaerobically Digested Sludges. Seven full-scale publicly owned treatment facilities were sampled several times to determine if bacte...

  1. Follicular fluid total antioxidant capacity levels in PCOS.

    Yilmaz, Nafiye; Inal, Hasan Ali; Gorkem, Umit; Sargin Oruc, Ayla; Yilmaz, Saynur; Turkkani, Ayten

    2016-07-01

    In this study, our aim was to assess total antioxidant capacity (TAC) levels in follicular fluid (FF) and their relationship to clinical pregnancy rates in PCOS patients undergoing assisted reproduction (ART). Twenty-two women with polycystic ovary syndrome (PCOS) (Group 1) and 41 women without PCOS (Group 2) were included in this study. Clinical and laboratory parameters and FF TAC levels were investigated. No statistically significant differences were found between the groups with regard to age and baseline parameters. Although we could not demonstrate a significant difference in FF TAC levels between the two groups (p=0.469), there was a significant positive correlation between FF TAC and clinical pregnancy rates, BMI, and the duration of infertility for the entire group (r=0.254, p=0.048; r=0.312, p=0.013; r=0.259, p=0.040; respectively). Owing to the correlation between FF TAC and the clinical pregnancy rates, further studies evaluating the impact of FF TAC levels on ART outcomes in patients with PCOS and other etiologies of infertility are needed. PMID:26911305

  2. Coliform detection in cheese is associated with specific cheese characteristics, but no association was found with pathogen detection.

    Trmčić, A; Chauhan, K; Kent, D J; Ralyea, R D; Martin, N H; Boor, K J; Wiedmann, M

    2016-08-01

    Coliform detection in finished products, including cheese, has traditionally been used to indicate whether a given product has been manufactured under unsanitary conditions. As our understanding of the diversity of coliforms has improved, it is necessary to assess whether coliforms are a good indicator organism and whether coliform detection in cheese is associated with the presence of pathogens. The objective of this study was (1) to evaluate cheese available on the market for presence of coliforms and key pathogens, and (2) to characterize the coliforms present to assess their likely sources and public health relevance. A total of 273 cheese samples were tested for presence of coliforms and for Salmonella, Staphylococcus aureus, Shiga toxin-producing Escherichia coli, Listeria monocytogenes, and other Listeria species. Among all tested cheese samples, 27% (75/273) tested positive for coliforms in concentrations >10cfu/g. Pasteurization, pH, water activity, milk type, and rind type were factors significantly associated with detection of coliforms in cheese; for example, a higher coliform prevalence was detected in raw milk cheeses (42% with >10cfu/g) compared with pasteurized milk cheese (21%). For cheese samples contaminated with coliforms, only water activity was significantly associated with coliform concentration. Coliforms isolated from cheese samples were classified into 13 different genera, including the environmental coliform genera Hafnia, Raoultella, and Serratia, which represent the 3 genera most frequently isolated across all cheeses. Escherichia, Hafnia, and Enterobacter were significantly more common among raw milk cheeses. Based on sequencing of the housekeeping gene clpX, most Escherichia isolates were confirmed as members of fecal commensal clades of E. coli. All cheese samples tested negative for Salmonella, Staph. aureus, and Shiga toxin-producing E. coli. Listeria spp. were found in 12 cheese samples, including 5 samples positive for L

  3. SORCE Level 3 Total Solar Irradiance Daily Average V016

    National Aeronautics and Space Administration — The Total Solar Irradiance (TSI) data set SOR3TSID contains the total solar irradiance (a.k.a solar constant) data collected by the Total Irradiance Monitor (TIM)...

  4. The Impact of Rainfall on Fecal Coliform Bacteria in Bayou Dorcheat (North Louisiana

    Paul B. Tchounwou

    2006-03-01

    Full Text Available Fecal coliform bacteria are the most common pollutant in rivers and streams. In Louisiana, it has been reported that 37% of surveyed river miles, 31% of lakes, and 23% of estuarine water had some level of contamination. The objective of this research was to assess the effect of surface runoff amounts and rainfall amount parameters on fecal coliform bacterial densities in Bayou Dorcheat in Louisiana. Bayou Dorcheat has been designated by the Louisiana Department of Environmental Quality as a waterway that has uses such as primary contact recreation, secondary contact recreation, propagation of fish and wildlife, agriculture and as being an outstanding natural resource water. Samples from Bayou Dorcheat were collected monthly and analyzed for the presence of fecal coliforms. Fecal coliforms isolated from these samples were identified to the species level. The analysis of the bacterial levels was performed following standard test protocols as described in Standard Methods for the Examination of Water and Wastewater. Information regarding the rainfall amounts and surface runoff amounts for the selected years was retrieved from the Louisiana Office of State Climatology. It was found that a significant increase in the fecal coliform numbers may be associated with average rainfall amounts. Possible sources of elevated coliform counts could include sewage discharges from municipal treatment plants and septic tanks, storm water overflows, and runoff from pastures and range lands. It can be concluded that nonpoint source pollution that is carried by surface runoff has a significant effect on bacterial levels in water resources.

  5. 美国环保署的大肠菌群(Coliforms)检测技术%Introduction of Coliforms test in US EPA.

    高瑞坤

    2006-01-01

    总大肠菌群(Total Coliforms)和大肠杆菌(E.coli)是当前国内、外环境监测部门评价水体污染程度的公认指标和主要监测项目之一.本文介绍了美国环保署应用Colilert酵素变色及荧光反应的方法,检测水中的总大肠菌群(Total Coliforms)和大肠杆菌(E.coli),可以在18-24小时获得检测结果,准确检测水中的总大肠菌群(Total Coliforms)和大肠杆菌(E.coli)的数量.

  6. Determination of total mercury in nuts at ultratrace level

    Highlights: • Direct analysis of Hg in nuts has been improved by a previous fat removal. • Comparison of cold vapour atomic fluorescence and direct analysis of Hg in nuts. • Mercury content in tree nuts was determined. - Abstract: Total mercury, at μg kg−1 level, was determined in different types of nuts (cashew nut, Brazil nuts, almond, pistachio, peanut, walnut) using a direct mercury analyser after previous sample defatting and by cold vapour atomic fluorescence spectrometry. There is not enough sensitivity in the second approach to determine Hg in previously digested samples due to the strong matrix effect. Mercury levels in 25 edible nut samples from Brazil and Spain were found in the range from 0.6 to 2.7 μg kg−1 by using the pyrolysis of sample after the extraction of the nut fat. The accuracy of the proposed method was confirmed by analysing certified reference materials of Coal Fly Ash-NIST SRM 1633b, Fucus-IAEA 140 and three unpolished Rice Flour NIES-10. The observed results were in good agreement with the certified values. The recoveries of different amounts of mercury added to nut samples ranged from 94 to 101%. RSD values corresponding to three measurements varied between 2.0 and 14% and the limit of detection and quantification of the method were 0.08 and 0.3 μg kg−1, respectively

  7. Determination of total mercury in nuts at ultratrace level

    Silva, Maria José da, E-mail: maryquimica@yahoo.com.br [Departamento de Química – Universidade Federal Rural de Pernambuco, Rue Dom Manoel de Medeiros s/n. Dois irmãos, 52171-900 Recife, PE (Brazil); Paim, Ana Paula S. [Departamento de Química Fundamental – Universidade Federal de Pernambuco, Cidade Universitária, 50740-550 Recife, PE (Brazil); Pimentel, Maria Fernanda [Departamento de Engenharia Química – Universidade Federal de Pernambuco, Recife, PE (Brazil); Cervera, M. Luisa; Guardia, Miguel de la [Department of Analytical Chemistry, Research Building, University of Valencia, 50th Dr. Moliner Street, E-46100 Burjassot, Valencia (Spain)

    2014-08-01

    Highlights: • Direct analysis of Hg in nuts has been improved by a previous fat removal. • Comparison of cold vapour atomic fluorescence and direct analysis of Hg in nuts. • Mercury content in tree nuts was determined. - Abstract: Total mercury, at μg kg{sup −1} level, was determined in different types of nuts (cashew nut, Brazil nuts, almond, pistachio, peanut, walnut) using a direct mercury analyser after previous sample defatting and by cold vapour atomic fluorescence spectrometry. There is not enough sensitivity in the second approach to determine Hg in previously digested samples due to the strong matrix effect. Mercury levels in 25 edible nut samples from Brazil and Spain were found in the range from 0.6 to 2.7 μg kg{sup −1} by using the pyrolysis of sample after the extraction of the nut fat. The accuracy of the proposed method was confirmed by analysing certified reference materials of Coal Fly Ash-NIST SRM 1633b, Fucus-IAEA 140 and three unpolished Rice Flour NIES-10. The observed results were in good agreement with the certified values. The recoveries of different amounts of mercury added to nut samples ranged from 94 to 101%. RSD values corresponding to three measurements varied between 2.0 and 14% and the limit of detection and quantification of the method were 0.08 and 0.3 μg kg{sup −1}, respectively.

  8. Contamination of water wells in Khoms city with pathogenic coliform bacteria

    240 Samples from 60 water wells in and around the area of city of Khoms city (in northwestern Libya) were analyzed over four successive seasons for count of faecal Coliform bacteria, of which antibiotic resistance was later assessed. Standard methods were used for analysis of faecla coliform bacteria. Water wells contained varying levels of faecal coliform bacteria ranging from a Most Probable Number of 0 to 1.8 x 103 cfu/100 ml, with zero isolates at autumn and winter seasons, while wells dug at private farms had the highest percentage of contamination, reaching 56.6% of wells in autumn, and also had the highest number of faecal coliform isolates, 1,8x103 cfu/100 ml, in spring and summer seasons, strains of isolated Escherichia coli were most sensitive to chloramphenicol, and most resistant to tetracycline. (author)

  9. Interaction of fecal coliforms with soil aggregates

    Land-applied manures may contain various contaminants that cause water pollution and concomitant health problems. Some of these pollutants are bacteria, and fecal coliforms (FC) have been widely used as an indicator of bacterial contamination. Experiments on bacteria attachment to soil are tradition...

  10. Phenotypic and phylogenetic identification of coliform bacteria obtained from 12 USEPA approved coliform methods

    Zhang, Ya

    2015-06-26

    The current definition of coliform bacteria is method-dependent, and when different culture-based methods are used, discrepancies in results can occur and affect the accuracy in identifying true coliforms. This study used an alternative approach to identify true coliforms by combing the phenotypic traits of the coliform isolates and the phylogenetic affiliation of 16S rRNA gene sequences together with the use of lacZ and uidA genes. A collection of 1404 isolates from 12 US Environmental Protection Agency approved coliform-testing methods were characterized based on their phylogenetic affiliations and responses to their original isolation medium and Lauryl Tryptose broth, m-Endo and MI agar media. Isolates were phylogenetically classified into 32 true coliform or targeted Enterobacteriaceae (TE) groups, and 14 non-coliform or non-targeted Enterbacteriaceae (NTE) groups. It was statistically shown that detecting true-positive (TP) events is more challenging than detecting true-negative (TN) events. Furthermore, most false-negative (FN) events were associated with four TE groups (i.e., Serratia group I, Providencia, Proteus, and Morganella), and most false-positive (FP) events with two NTE groups, Aeromonas and Plesiomonas. In Escherichia coli testing, 18 out of 145 E. coli isolates identified by those enzymatic methods were validated as FNs. The reasons behind the FP and FN reactions could be explained through the analysis of the lacZ and uidA gene. Overall, combining the analyses of 16S rRNA, lacZ and uidA genes with the growth responses of TE and NTE on culture-based media is an effective way to evaluate the performance of coliform detection methods.

  11. Effects of oral prazosin on total plasma digoxin levels.

    Copur, S; Tokgözoğlu, L; Oto, A; Oram, E; Uğurlu, S

    1988-01-01

    Prazosin and digoxin are frequently coadministered in clinical practice. To determine the effects of oral prazosin treatment on steady-state digoxin levels, 20 patients receiving a constant maintenance dose of digoxin, who had normal renal and liver functions and were not receiving any other treatment, were given 5 mg of prazosin for 3 days. Plasma digoxin levels were measured before, on days 1 and 3 of prazosin treatment, and after prazosin had been discontinued. It was found that prazosin significantly increased plasma digoxin levels. On discontinuation of prazosin digoxin levels returned to their previous values. PMID:3371837

  12. THAWING PROCEDURES FOR HOSPITAL-MADE ENTERAL FEEDINGS: SURVIVAL OF COLIFORM AND MESOPHILIC AEROBIC BACTERIA

    KATHIA ROSSI ROLIM LOPES

    2009-07-01

    Full Text Available

    ABSTRACT: This study had the purpose to observe the effect of thawing procedures on survival of coliform and mesophilic aerobic bacteria in hospital-made enteral feedings. The samples are represented by three different lots. The tests were realized in three moments: immediately after the sample preparation and after freezing during 1 or 2 months. The thawing procedures were denominated convencional and alternative. The first, used by hospital, utilizes water bath at 50ºC, considering the time spent from the total thawing to its distribution in the infirmaries. The second was the fast thawing made by microwaving. The results showed that the reduction of the mesophiles and coliform was related to the time the samples were frozen. The results obtained indicate an advantage of the alternative method, which presented lower total and fecal coliform counts than the conventional one. KEYWORDS: Enteral feedings; thawing; food microbiology.

  13. Comparison of serum leptin, glucose, total cholesterol and total protein levels in fertile and repeat breeder cows

    Saime Guzel

    2014-12-01

    Full Text Available In the present study we measured serum glucose, leptin, total cholesterol and total protein concentrations in repeat breeder cows and compared them with fertile cows. For this aim, 20 repeat breeder cows and 20 fertile cows were used as material. Repeat breeder cows were found to have lower levels of leptin and glucose as compared with fertile ones. No significant differences in total cholesterol and total protein levels were observed between the two groups. No significant correlation of leptin with glucose, total cholesterol and total protein was observed in fertile and repeat breeder cows. Low concentrations of glucose and leptin can have some effects on reproductive problems as repeat breeder and help to understand potential mechanisms impairing fertility in repeat breeder cows.

  14. Total daily energy expenditure and activity level in anorexia nervosa.

    Casper, R C; Schoeller, D A; Kushner, R; Hnilicka, J; Gold, S T

    1991-05-01

    Clinical reports consistently comment on high physical activity for anorexia nervosa patients but provide few quantitative measurements. To assess activity, total daily energy expenditure (TDEE) by doubly labeled water, basal metabolic rate (BMR), and thermic effect of meals (TEM) were measured in six female outpatients with anorexia nervosa (67% of ideal body weight) and age-, sex-, and height-matched to six control subjects. Anorexia nervosa patients expended more energy as physical activity than did control subjects [0.084 +/- 0.012 vs 0.044 +/- 0.008 MJ/kg body wt, respectively (20.1 +/- 3.0 vs 10.5 +/- 1.9 kcal/kg body wt, respectively), P less than 0.04], although they had a lower BMR [4.17 +/- 0.37 vs 5.52 +/- 0.15 MJ/d, respectively (997 +/- 89 vs 1319 +/- 37 kcal/d, respectively), P less than 0.01]. TDEE and TEM were similar in both groups. There was a reduction in serum triiodothyronine (T3; 1.20 +/- 0.15 vs 2.04 +/- 0.13 nmol/L, respectively; P less than 0.003) and a slight reduction in serum thyroxine (T4); reverse T3, thyrotropin, free T4, serum cortisol, and adrenocorticotropin values were normal. BMR correlated with total body weight and fat-free mass. These results provide quantitative evidence for increased physical activity in anorexia nervosa despite profound underweight and hypometabolism. PMID:1850575

  15. Low serum total nitrite and nitrate levels in severe leptospirosis

    Kalugalage, Thilini; Rodrigo, Chaturaka; Vithanage, Thamal; Somaratne, Pranitha; de Silva, H. Janaka; Handunnetti, Shiroma; Rajapakse, Senaka

    2013-01-01

    Background The relationship between inducible nitric oxide synthatase activity and disease severity in leptospirosis is unclear. Nitric oxide is converted to nitrites and nitrates, thus nitrite and nitrate levels (NOx) in serum are considered surrogate markers for nitric oxide. NOx are excreted through the kidneys, and elimination is diminished in renal impairment. We assessed the correlation of NOx with disease severity in patients with leptospirosis, compared with healthy controls and non-l...

  16. Elimination of coliforms and Salmonella spp. in sheep meat by gamma irradiation treatment

    Luciana Salles Vasconcelos Henriques; Fábio da Costa Henry; João Batista Barbosa; Silvania Alves Ladeira; Silvia Menezes de Faria Pereira; Isabela Maria da Silva Antonio; Gina Nunes Teixeira; Meire Lelis Leal Martins; Helio de Carvalho Vital; Dália dos Prazeres Rodrigues; Eliane Moura Falavina dos Reis

    2014-01-01

    This study aimed at evaluating the bacteriological effects of the treatment of sheep meat contaminated with total coliforms, coliforms at 45 °C and Salmonella spp. by using irradiation at doses of 3 kGy and 5 kGy. Thirty sheep meat samples were collected from animals located in Rio de Janeiro State, Brazil, and then grouped in three lots including 10 samples: non-irradiated (control); irradiated with 3 kGy; and irradiated with 5 kGy. Exposure to gamma radiation in a 137Cs source-driven irradi...

  17. Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants

    Oggero Roberto

    2011-06-01

    Full Text Available Abstract Background Infantile colic is a common disturb within the first 3 months of life, nevertheless the pathogenesis is incompletely understood and treatment remains an open issue. Intestinal gas production is thought to be one of the causes of abdominal discomfort in infants suffering from colic. However, data about the role of the amount of gas produced by infants' colonic microbiota and the correlation with the onset of colic symptoms are scanty. The benefit of supplementation with lactobacilli been recently reported but the mechanisms by which they exert their effects have not yet been fully defined. This study was performed to evaluate the interaction between Lactobacillus spp. strains and gas-forming coliforms isolated from stools of colicky infants. Results Strains of coliforms were isolated from stools of 45 colicky and 42 control breastfed infants in McConkey Agar and identified using PCR with species-specific primers, and the BBL™ Enterotube™ II system for Enterobacteriaceae. Gas-forming capability of coliforms was assessed in liquid cultures containing lactose as sole carbon source. The average count of total coliforms in colicky infants was significantly higher than controls: 5.98 (2.00-8.76 log10 vs 3.90 (2.50-7.10 CFU/g of faeces (p = 0.015. The following strains were identified: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter aerogenes, Enterobacter cloacae and Enterococcus faecalis. Then, 27 Lactobacillus strains were tested for their antagonistic effect against coliforms both by halo-forming method and in liquid co-cultures. Lactobacillus delbrueckii subsp.delbrueckii DSM 20074 and L. plantarum MB 456 were able to inhibit all coliforms strains (halo-forming method, also in liquid co-cultures, thus demonstrating an antagonistic activity. Conclusions This study shows that two out of 27 strains of Lactobacillus examined possess an antimicrobial effect against six species of gas-forming coliforms

  18. 33 CFR 159.123 - Coliform test: Type I devices.

    2010-07-01

    ... (CONTINUED) POLLUTION MARINE SANITATION DEVICES Design, Construction, and Testing § 159.123 Coliform test... milliliters when tested in accordance with 40 CFR part 136. (b) The 40 samples must be taken from the device... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Coliform test: Type I...

  19. Modeling seasonal variability of fecal coliform in natural surface waters using the modified SWAT

    Cho, Kyung Hwa; Pachepsky, Yakov A.; Kim, Minjeong; Pyo, JongCheol; Park, Mi-Hyun; Kim, Young Mo; Kim, Jung-Woo; Kim, Joon Ha

    2016-04-01

    Fecal coliforms are indicators of pathogens and thereby, understanding of their fate and transport in surface waters is important to protect drinking water sources and public health. We compiled fecal coliform observations from four different sites in the USA and Korea and found a seasonal variability with a significant connection to temperature levels. In all observations, fecal coliform concentrations were relatively higher in summer and lower during the winter season. This could be explained by the seasonal dominance of growth or die-off of bacteria in soil and in-stream. Existing hydrologic models, however, have limitations in simulating the seasonal variability of fecal coliform. Soil and in-stream bacterial modules of the Soil and Water Assessment Tool (SWAT) model are oversimplified in that they exclude simulations of alternating bacterial growth. This study develops a new bacteria subroutine for the SWAT in an attempt to improve its prediction accuracy. We introduced critical temperatures as a parameter to simulate the onset of bacterial growth/die-off and to reproduce the seasonal variability of bacteria. The module developed in this study will improve modeling for environmental management schemes.

  20. Serum levels of total IgE and soluble CD23 in bronchial asthma

    G. Di Lorenzo

    1996-01-01

    Full Text Available The aim of the present study was to compare, during the pollen season, serum levels of total IgE and soluble CD23 (sCD23 from patients with allergic bronchial asthma, with those from healthy subjects. Significantly higher levels of total IgE and sCD23 were found in patients with asthma compared to the control group. Both in normal controls and in asthmatic patients, a significant correlation was shown between the levels of these two molecules. In asthmatic patients, significant correlations were found for both total IgE and sCD23, with lung function measured as bronchial responsiveness to inhaled methacholine. These results suggest that in asthmatic patients, in addition to the study of total serum IgE levels, the assessment of sCD23 serum levels may be helpful in the evaluation of disease activity.

  1. Could Low Total and Free Testosterone Levels be risk factor for Achilles Tendon Ruptures in Males

    Abebe, Ermias Shawel; Tarkin, Ivan; Prisk, Victor

    2013-01-01

    Objectives: Age related decline in sex hormone levels has been associated with decreased muscle mass, bone density, and changes in metabolism in both males and females[1-3]. Although the effect of estrogen levels on tendon size and architecture has been studied in females [4,5], the influence of testosterone levels on tendons in males is unknown. The purpose of the present study was to retrospectively compare free testosterone (FT) and total testosterone (TT) level in males diagnosed with Ach...

  2. Method of Detecting Coliform Bacteria from Reflected Light

    Vincent, Robert K. (Inventor)

    2014-01-01

    The present invention relates to a method of detecting coliform bacteria in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.

  3. Frequency distribution of coliforms in water distribution systems.

    Christian, R R; Pipes, W O

    1983-01-01

    Nine small water distribution systems were sampled intensively to determine the patterns of dispersion of coliforms. The frequency distributions of confirmed coliform counts were compatible with either the negative-binomial or the lognormal distribution. They were not compatible with either the Poisson or Poisson-plus-added zeroes distribution. The implications of the use of the lognormal distributional model were further evaluated because of its previous use in water quality studies. The geo...

  4. A follow up on the distribution of coliform bacteria along the Jordanian coastline of the gulf of Aq aba

    A survey of the occurrence of enteric indicator organisms (e. g. total and fecal coliform) and enteric pathogenic bacteria (Salmonella sp.) was undertaken at ten sites, along the northern coast of the Gulf of Aq aba (Jordan)m during the period October 1994 to September 1995. Both total coliforms and Escherichia coli counts were within the accepted international standards for marine bathing water. the mean value per 100 ml seawater ranged between 18 to 70(n=12 months) for total coliforms, 1 to 27 (n=8 mon thus) for Escherichia coli and 1 to 9 (n = 8 months) for Salmonella species. The analysis of va rance for total coliforms revealed a highly significant temporal variations (P<0.001), but no significant spatial differences were found .Whereas, Salmonella sp. counts showed significant spatial variations (P<0.001), with the Border site being significantly different from the other sites except the Palace site. These results suggest that the hygienic water quality at the Jordanian side of the Gulf of Aq aba, is good all over the year although the sewage discharge from Ei lat might cause a health hazard as to be the major source of enteric pathogenic bacteria. (authors). 13 refs., 4 figs., 1 tab

  5. INFLUENCE OF MEAT MATURATION TO THE PRESENCE OF COLIFORM BACTERIA

    Ľubomír Lopašovský

    2012-12-01

    Full Text Available Normal 0 21 false false false SK JA X-NONE The aim of our study was detection of coliforms bacteria and pH changes in the process of beef maturation. The number of coliforms bacteria were lower as 1 log cfu.g-1 in four samples and the highest coliforms bacteria count was 3.1 log cfu.g-1 after 1-st week of meat maturation. Average number of coliforms bacteria was lower as 1.43 log cfu.g-1.  The pH values of meat varied from 5.5 to 6.1 after 1-st week. Average value of pH was 5.75.  The number of coliforms bacteria were from 2.61 log cfu.g-1 to 3.35 log cfu.g-1 after 2-nd week of meat maturation. Average number of coliforms bacteria was 3.17 log cfu.g-1. The pH values of meat were from 6.0 to 6.2 after 2-nd week of meat maturation. Average value of pH was 6.05. doi:10.5219/181

  6. Serum total bilirubin levels and coronary heart disease--Causal association or epiphenomenon?

    Kunutsor, Setor K

    2015-12-01

    Observational epidemiological evidence supports a linear inverse and independent association between serum total bilirubin levels and coronary heart disease (CHD) risk, but whether this association is causal remains to be ascertained. A Mendelian randomization approach was employed to test whether serum total bilirubin is causally linked to CHD. The genetic variant rs6742078--well known to specifically modify levels of serum total bilirubin and accounting for up to 20% of the variance in circulating serum total bilirubin levels--was used as an instrumental variable. In pooled analysis of estimates reported from published genome-wide association studies, every copy of the T allele of rs6742078 was associated with 0.42 standard deviation (SD) higher levels of serum total bilirubin (95% confidence interval, 0.40 to 0.43). Based on combined data from the Coronary Artery Disease Genome wide Replication and Meta-analyses and the Coronary Artery Disease (C4D) Genetics Consortium involving a total of 36,763 CHD cases and 76,997 controls, the odds ratio for CHD per copy of the T allele was 1.01 (95% confidence interval, 0.99 to 1.04). The odds ratio of CHD for a 1 SD genetically elevated serum total bilirubin level was 1.03 (95% confidence interval, 0.98 to 1.09). The current findings casts doubt on a strong causal association of serum total bilirubin levels with CHD. The inverse associations demonstrated in observational studies may be driven by biases such as unmeasured confounding and/or reverse causation. However, further research in large-scale consortia is needed. PMID:26408227

  7. Total Immunoglobulin E Levels and Dengue Infection on San Andrés Island, Colombia

    Míguez-Burbano, María José; Jaramillo, Carlos A.; Palmer, Carol J.; Shor-Posner, Gail; Velásquez, Luz Stella; Lai, Hong; Baum, Marianna K

    1999-01-01

    An evaluation of total immunoglobulin E (IgE) and dengue serostatus in 168 subjects from San Andrés Island, Colombia, revealed altered levels of IgE in 89% of the population. IgE levels were higher in patients with a history of dengue or with a current secondary or current primary infection than in subjects with no exposure (P = 0.01). Dengue infection accounted for 23% of the variation in IgE levels.

  8. Adult total wellness: group differences based on sitting time and physical activity level

    2014-01-01

    Background An increasing body of evidence associates a high level of sitting time with poor health outcomes. The benefits of moderate to vigorous-intensity physical activities to various aspects of health are now well documented; however, individuals may engage in moderate-intensity physical activity for at least 30 minutes on five or more days of the week and still exhibit a high level of sitting time. This purpose of this study was to examine differences in total wellness among adults relative to high/low levels of sitting time combined with insufficient/sufficient physical activity (PA). The construct of total wellness incorporates a holistic approach to the body, mind and spirit components of life, an approach which may be more encompassing than some definitions of health. Methods Data were obtained from 226 adult respondents (27 ± 6 years), including 116 (51%) males and 110 (49%) females. Total PA and total sitting time were assessed with the International Physical Activity Questionnaire (IPAQ) (short-version). The Wellness Evaluation of Lifestyle Inventory was used to assess total wellness. An analysis of covariance (ANCOVA) was utilised to assess the effects of the sitting time/physical activity group on total wellness. A covariate was included to partial out the effects of age, sex and work status (student or employed). Cross-tabulations were used to show associations between the IPAQ derived high/low levels of sitting time with insufficient/sufficient PA and the three total wellness groups (i.e. high level of wellness, moderate wellness and wellness development needed). Results The majority of the participants were located in the high total sitting time and sufficient PA group. There were statistical differences among the IPAQ groups for total wellness [F (2,220) = 32.5 (p <0.001)]. A Chi-square test revealed a significant difference in the distribution of the IPAQ categories within the classification of wellness [χ2 (N = 226) = 54.5, p < .001

  9. The Seasonality of Fecal Coliform Bacteria Pollution and its Influence on Closures of Shellfish Harvesting Areas in Mississippi Sound

    Tchounwou, Paul B.; Scott Gordon; Paulinus Chigbu

    2005-01-01

    Runoff from agricultural lands and farm animal feedlots is one of the major sources of fecal coliforms in surface waters, and fecal coliform (FC) bacteria concentrations tend to vary with season because of seasonal variations in climatic factors. However, El Niño -Southern Oscillation (ENSO) events may affect the extent and patterns of seasonality in FC levels in coastal waters. Water quality monitoring data for shellfish management collected during El Niño (1990, 1992, 1997), and La Ni...

  10. Effect of Mentha Piperita L. Powder on the Overall Acceptability, Coliforms, and Molds and Yeasts Counts of Borujerd Domestic Cheese

    FADAEİ NOGHANİ, Vajiheh; SAMETİ, Sadrieh

    2015-01-01

    Abstract. In this study, the antimicrobial effects of plant powder Mentha Piperita L. in at the levels of 0.1, 0.2 and 0.3 % on preventing the growth of mold, yeast and coliforms, as well as the, overall acceptability, pH and acidity of Borujerd domestic cheese samples during the cold storage were studied. The findings showed that with increasing the percentage of Mentha Piperita L. powder in cheese samples, the number of coliforms and mold and yeast, titratable acidity and overall acceptabil...

  11. Evaluación de la contaminación del yogur por coliformes, E. coli y enterococos

    Jordano-Salinas, R.

    1984-01-01

    We have done a research work on the evolution of the presence of coliforms, E. coli and enterococci in yoghurt which was kept at 72 C for 40 days. We have dealt with 100 samples of two varieties: natural and strawberry flavour yoghourt, both of them from a very well known commercial make. It ..has also been measured the pH and acidity of them. It has been checked the presence of coliforms and of E. coli, and in ten days time, we have noticed their extinction which was due to a higher level of...

  12. Elimination of coliforms and Salmonella spp. in sheep meat by gamma irradiation treatment

    Luciana Salles Vasconcelos Henriques

    2013-12-01

    Full Text Available This study aimed at evaluating the bacteriological effects of the treatment of sheep meat contaminated with total coliforms, coliforms at 45 °C and Salmonella spp. by using irradiation at doses of 3 kGy and 5 kGy. Thirty sheep meat samples were collected from animals located in Rio de Janeiro State, Brazil, and then grouped in three lots including 10 samples: non-irradiated (control; irradiated with 3 kGy; and irradiated with 5 kGy. Exposure to gamma radiation in a 137Cs source-driven irradiating facility was perfomed at the Nuclear Defense Section of the Brazilian Army Technological Center (CTEx in Rio de Janeiro. The samples were kept under freezing temperature (-18 °C until the analyses, which occurred in two and four months after irradiation. The results were interpreted by comparison with the standards of the current legislation and demonstrated that non-irradiated samples were outside the parameters established by law for all groups of bacteria studied. Gamma irradiation was effective in inactivating those microorganisms at both doses tested and the optimal dose was achieved at 3 kGy. The results have shown not only the need for sanitary conditions improvements in slaughter and processing of sheep meat but also the irradiation effectiveness to eliminate coliform bacteria and Salmonella spp.

  13. Salmonelas e coliformes fecais em águas de bebida para animais

    Souza Luiz Carlos de

    1992-01-01

    Full Text Available De um total de 402 propriedades rurais do Município de Botucatu, SP (Brasil com atividades de exploração pecuária, foram sorteadas 60 (15% pelo processo de amostragem probabilística simples. Nestas 60 propriedades localizaram-se 113 bebedouros que eram os mais utilizados pelos animais para sua dessedentação. Foram colhidas amostras de água desses bebedouros visando a pesquisa de bactérias do gênero Salmonella, a determinação do Número Mais Provável (NMP de bactérias coliformes fecais e a verificação do pH e da temperatura. Obteve-se isolamento positivo para salmonelas nas amostras de água de 15 (13,27% bebedouros correspondentes a 12 (20% das 60 propriedades visitadas, identificando-se os seguintes sorotipos: S. dublin, S. newport, S. madelia, S. IV 43:g, z57: -, S. saphra, S. glostrup, S. IV ochsenzool, S. I 9, 12:i: - e dois novos sorotipos S. IV 41:z52: - e S. IV 50:d:-. Das 113 amostras de água analisadas, 14 (12,39% apresentaram-se com NMP/100 mL de coliformes fecais acima de 4.000. Não foi constatada associação entre o isolamento positivo de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000. O isolamento de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000 deu-se em temperaturas superiores a 18degreesC e em pH entre 6,0 e 7,0.

  14. Salmonelas e coliformes fecais em águas de bebida para animais

    Luiz Carlos de Souza

    1992-10-01

    Full Text Available De um total de 402 propriedades rurais do Município de Botucatu, SP (Brasil com atividades de exploração pecuária, foram sorteadas 60 (15% pelo processo de amostragem probabilística simples. Nestas 60 propriedades localizaram-se 113 bebedouros que eram os mais utilizados pelos animais para sua dessedentação. Foram colhidas amostras de água desses bebedouros visando a pesquisa de bactérias do gênero Salmonella, a determinação do Número Mais Provável (NMP de bactérias coliformes fecais e a verificação do pH e da temperatura. Obteve-se isolamento positivo para salmonelas nas amostras de água de 15 (13,27% bebedouros correspondentes a 12 (20% das 60 propriedades visitadas, identificando-se os seguintes sorotipos: S. dublin, S. newport, S. madelia, S. IV 43:g, z57: -, S. saphra, S. glostrup, S. IV ochsenzool, S. I 9, 12:i: - e dois novos sorotipos S. IV 41:z52: - e S. IV 50:d:-. Das 113 amostras de água analisadas, 14 (12,39% apresentaram-se com NMP/100 mL de coliformes fecais acima de 4.000. Não foi constatada associação entre o isolamento positivo de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000. O isolamento de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000 deu-se em temperaturas superiores a 18°C e em pH entre 6,0 e 7,0.

  15. Inactivation of Escherichia coli and coliform bacteria in traditional brass and earthernware water storage vessels.

    Tandon, Puja; Chhibber, Sanjay; Reed, Robert H

    2005-07-01

    The detection and enumeration of indicator bacteria such as Escherichia coli is used to assess the extent of faecal contamination of drinking water. On the basis of this approach, the effectiveness of storing water contaminated with faecal indicator bacteria in brass or earthern vessels (mutkas) of the type used in rural India have been investigated. Suspensions of bacteria in sterile distilled water were maintained for up to 48 h in each vessel and enumerated by surface plate counts on nutrient agar (non-selective) and several selective coliform media at 37 degrees C either under standard aerobic conditions, or under conditions designed to neutralise reactive oxygen species (ROS), e.g. using an anaerobic cabinet to prepare plates of pre-reduced growth medium or by inclusion of sodium pyruvate in the growth medium, with incubation of aerobically-prepared plates in an anaerobic jar. The counts obtained for E. coli decreased on short-term storage in a brass mutka; counts for selective media were lower than for equivalent counts for non-selective medium, with ROS-neutralised conditions giving consistently higher counts than aerobic incubation. However, after 48 h, no bacteria were cultivable under any conditions. Similar results were obtained using water from environmental sources in the Panjab, and from rural households where brass and earthern mutkas are used for storage of drinking water, with enumeration on selective coliform media (presumptive total coliforms). In all cases results indicated that, while storage of water in a brass mutka can inactivate E. coli and coliforms over a 48 h period, standard aerobic plate counting using selective media may not be fully effective in enumerating sub-lethally damaged bacteria. PMID:15928975

  16. Effect of elevated total CoA levels on metabolic pathways in cultured hepatocytes

    Livers from fasted rats have 30% higher total CoA levels than fed rats. To determine whether this increase of total CoA influences metabolism, the rates of gluconeogenesis, fatty acid oxidation and ketogenesis were measured in hepatocytes with cyanamide (CYM) or pantothenate (PA) deficient medium used to vary total CoA levels independently of hormonal status. Primary cultures of rat hepatocytes were incubated 14 hrs with Bt2 cAMP, dexamethasone + theophylline in PA deficient medium or with CYM (500 μM) + PA, rinsed and preincubated 0.5 hr to remove the CYM. Hepatocytes treated with CYM had total CoA levels 10-24% higher than PA deficient cells and lower rates of glucose production from lactate + pyruvate (L/P) or from alanine (0.23 +/- 0.05 and 0.089 +/- 0.02 μm/mg protein, respectively in CYM treated cells compared to 0.33 +/- 0.06 and 0.130 +/- 0.006 in PA deficient cells). This decrease was not due to CYM per se, as the direct addition of CYM stimulated glucose production from L/P. CYM treated cells with 15-40% higher total CoA and 30% higher fatty acyl-CoA levels had the same rates of [14C]-palmitate oxidation as PA deficient cells. However, rates of ketogenesis were lower in CYM treated cells (163 +/- 11 nm/mg compared to 217 +/- 14 nm/mg protein). These results suggest that physiological alterations of hepatic total CoA levels are not necessary for fasting rates of gluconeogenesis, fatty acid oxidation and ketogenesis

  17. Characteristics of High-density Lipoprotein Subclasses Distribution for Subjects with Desirable Total Cholesterol Levels

    Xu Yanhua; Liu Yinghui; Fu Mingde; Long Shiyin; Tian Li; Jia Lianqun

    2011-01-01

    Abstract Background To investigate alteration of high density lipoproteins (HDL) subclasses distribution in different total cholesterol (TC) levels, mainly the characteristics of HDL subclasses distribution in desirable TC levels and analyze the related mechanisms. Methods ApoA-I contents of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection. 486 Chinese Adults subjects were assigned to different TC groups according to the third Report of N...

  18. Serum total sialic acid, lipid peroxidation, and glutathione reductase levels in patients with rheumatoid arthritis

    Mohan, Surapaneni Krishna; PRIYAV, Vishnu

    2010-01-01

    The changes in the serum sialic acid levels, total lipid peroxidation products (MDA), and glutathione reductase activity were estimated in patients with rheumatoid arthritis. Serum Sialic acid is known as a parameter of inflammation. This work was undertaken to assess the potential role of sialic acid as well as oxidative stress and antioxidant status in patients with rheumatoid arthritis. Materials and methods: The levels of these parameters in serum were studied in 52 subjects with rheuma...

  19. Evolution of extreme Total Water Levels along the northern coast of the Iberian Peninsula

    D. F. Rasilla Álvarez

    2011-02-01

    Full Text Available This paper assesses the evolution of storminess along the northern coast of the Iberian Peninsula through the calculation of extreme (1% Total Water Levels (eTWL on both observed (tide gauge and buoy data and hindcasted (SIMAR-44 data. Those events were first identified and then characterized in terms of oceanographic parameters and atmospheric circulation features. Additionally, an analysis of the long-term trends in both types of data was performed. Most of the events correspond to a rough wave climate and moderate storm surges, linked to extratropical disturbances following a northern track. While local atmospheric conditions seem to be evolving towards lesser storminess, their impact has been balanced by the favorable exposure of the northern coast of the Iberian Peninsula to the increasing frequency and strength of distant disturbances crossing the North Atlantic. This evolution is also correctly reproduced by the simulated long-term evolution of the forcing component (meteorological sea level residuals and wave run up of the Total Water Level values calculated from the SIMAR 44 database, since sea level residuals have been experiencing a reduction while waves are arriving with longer periods. Finally, the addition of the rate of relative sea level trend to the temporal evolution of the atmospheric forcing component of the Total Water Level values is enough to simulate more frequent and persistent eTWL.

  20. Domestic exposure to fungi and total serum IgE levels in asthmatic children.

    Su, Huey-Jen Jenny; Wu, Pei-Chih; Lei, Huan-Yao; Wang, Jiu-Yao

    2005-08-14

    We measured the number of airborne, viable fungi and house dust mite (HDM) allergen levels in the homes of a group of asthmatic children. Blood samples were drawn and the amounts of total and specific serum IgE were determined. The association between the number of fungal colonies, dust mite allergen exposure, and specific and total IgE was evaluated. The number of viable airborne fungi was high (20,543 CFU/m(3)) in those investigated houses. Der p1 concentrations on child's mattress exceeding 2 microg/g were found in 78.6% of the houses. A quantitative dose-response relationship was demonstrated between the exposure to viable, airborne molds and the amount of total IgE (r = 0.4399 and P = .0249) and the level was further increased in children with coexposure to viable fungi and HDM. PMID:16106103

  1. Coliform bacteria as in indicator of sewerage water mixing with drinking water sources in Rawalpindi city

    The coliform group of bacteria are consider to be one of the prominent indicators of surface/groundwater pollution as their presence in drinking water sources shows that water has been in contact with soil, plants, septic tanks or sewerage lines/drains. As a part of surface/groundwater pollution studies in various areas of Rawalpindi city coliform bacteria have been determined in the available drinking sources to evaluate their possible connection with the nearby septic tanks and sewerage lines/drains. Selective water samples were tapped from 72 domestic dug wells, and 98 municipal corporation tube-wells and associated water supply lines in some poorly drained areas of Rawalpindi. These samples were analyzed using membrane filter technique. In general, the sampled areas have indicated poor water quality w.r.t. coliform activity. 52% samples of the collected samples have indicated presence of Ecoli. Of these, 73% samples mostly collected from the poorly drained areas have shown significant counts of Ecoli. These water are rendered unfit for drinking purposes. Thirteen water samples collected indicated toxic levels of Ecoli in the municipal water supply caused due to a known leakage in the main domestic water supply line. The presence of coliform in the tube-well water supply taps are thus attributed to ruptures in the underground water supply lines. Present study reveals that general sanitary condition and water quality in the city are poor and that there is an urgent need of improvement in the water treatment and distribution systems by the concern quaters. (Orig./A.B.)

  2. Problems with radiometric coliform assays. Final report, 16 Dec 1974--31Mar 1976

    Previte, J.J.; Roskey, C.T.

    1976-04-30

    A study has been made of the feasibility of adapting conventional media for coliform assays to rapid radiometric techniques. A variety of media included Lauryl Tryptose, British MacConkey, m-Endo-MF, Nutrient and Brilliant Green Bile broths. Radioactive substrates included 1-C14 Lactose, 1 and 6C14 glucose and C14 formate. One ml samples of 24-hour cultures diluted in phosphate buffered water were inoculated into 9 ml of radiometric medium in vaccine capped vials. They were incubated at 35C on a rotary shaker at 200 rpm. The time to detect a significant level of C(14)O2 varied inversely with the logarithm of the inoculum of several coliform species. A lack of specificity of the techniques was revealed when several strains of Escherichia coli were studied individually in addition to strains of Enterobacter agglomerans, Pseudomonas aeruginosa, Proteus inconstans, Proteus morganii and Salmonella St. Paul. Non-coliform species produced C(14)O2 in the radiometric media tested. (Author) (GRA)

  3. Hypogonadism and subnormal total testosterone levels in men with type 2 diabetes mellitus

    Objective: To determine the frequency of testosterone deficiency syndrome (TDS) in men with type 2 diabetes mellitus (DM). Study Design: A cross-sectional study. Place and Duration of Study: The Gbagada General Hospital, Gbagada Lagos, Nigeria, from December 2009 to May 2010. Methodology: A total of 203 men with type 2 DM aged 30-86 years were evaluated for TDS by a combination of positive ADAM (androgen deficiency in the ageing male) scores and subnormal total testosterone levels. Mild testosterone deficiency referred to total testosterone (TT) levels of 8-12 nmol/L with symptoms of hypogonadism and severe testosterone deficiency referred to TT levels < 8 nmol/L with or without hypogonadal symptoms. Results: Mild and severe TDS were present in 18.3% and 17% respectively of the study subjects. Commonly occurring clinical parameters of the TDS were erectile dysfunction and loss of libido, which were documented in 63% and 60% respectively in the study subjects. The majority of clinical features of the TDS were comparable in men with and without the TDS. Conclusion: About a third of men with type 2 DM had the TDS. The majority of the symptoms of hypogonadism are largely non-specific and their occurrence is comparable in men with and without low testosterone levels; thus, underscoring the need to have testosterone levels determined in men presenting with such symptoms. (author)

  4. Total and free valproic acid: plasma level/dose ratio in monotherapy.

    Abadín, J A; Durán, J A; Sánchez, A; Serrano, J S

    1991-04-01

    Free plasma level/dose ratio of valproic acid (L/D-F) can be more effective than total plasma level/dose ratio (L/D-T) in adjusting dosage regimens. The influence of age, dose, and plasma concentration have been studied on L/D-T and L/D-F ratios. L/D-T and L/D-F ratios from 67 outpatients under long-term monotherapy were obtained. Analytical data was carried out by fluorescent polarized immunoassay. L/D-T and L/D-F ratios do not vary according to age. L/D-T and L/D-F ratios decreased while the dosage increased; both ratios increased with an increase in total plasma level of valproic acid. Significant differences were found between L/D-T and L/D-F ratios. Dose and interindividual variations are the factors which most influence L/D ratios of valproic acid. PMID:2051846

  5. Tear ascorbic acid levels and the total antioxidant status in contact lens wearers: A pilot study

    Venkata Sai

    2009-01-01

    Full Text Available Aims: The tear ascorbate owing to its high concentration, functions as an effective antioxidant against the oxidative damage of cornea. Contact lens wearers (CLW are prone to oxidative stress due to the lens-induced hypoxic conditions. A pilot study was done to compare the tear ascorbic acid level and the total antioxidant capacity give as in normal and CLW. Materials and Methods: In this study 21 CLW (Mean age 23 ± 3 years ; M-2, F-19, who were daily wear users, with duration of wear not more than four years, along with age-matched 28 controls (Mean age 28 ± 3 ; M-15, F-13 were recruited in the study for collection of reflex tears using Schirmer′s strip. Ascorbic acid in tears was determined using high-performance liquid chromatography (HPLC, total antioxidant capacity (TAC and total protein assay by spectrophotometric analysis. Results: CLW showed no significant change in the tear ascorbic acid levels (0.4 ± 0.26 mM compared to the control subjects (0.61 ± 0.59 mM. The amount of ascorbic acid in tears did not correlate with the TAC or the total protein of the tears. The mean TAC in CLW was 0.69 ± 0.16 mM, with a total protein of 1.35 ± 0.46 mg/ml while in controls it was 0.7 ± 0.18 mM and 1.21 ± 0.47 mg/ml respectively . Conclusions: Soft contact lens wear did not show any significant change in tear ascorbic acid, TAC and total protein levels compared to controls.

  6. Domestic Exposure to Fungi and Total Serum IgE Levels in Asthmatic Children

    Huey-Jen Jenny Su; Pei-Chih Wu; Huan-Yao Lei; Jiu-Yao Wang

    2005-01-01

    We measured the number of airborne, viable fungi and house dust mite (HDM) allergen levels in the homes of a group of asthmatic children. Blood samples were drawn and the amounts of total and specific serum IgE were determined. The association between the number of fungal colonies, dust mite allergen exposure, and specific and total IgE was evaluated. The number of viable airborne fungi was high (20 543 CFU/m3) in those investigated houses. Der p1 concentrations on child's mattress exceeding ...

  7. Pengujian Bakteri Coliform Pada Air Sumur Di Medan Johor

    Harahap, Lola Alia Yolanda

    2013-01-01

    Water is an essential material in life. Water is a major means to increase public health. The spread of water-borne desease can be. Water pollution canbe caused by the entry of human and animal feces. It can also be caused by the return of waste water in to the well directly or through leaks and openings where the soil. Coliform is a group of bacteria used as an indicator of pollution and dirt are not good conditions for water. The purpose of testing is to test Coliform bacteria on one of wel...

  8. CORRELATION BETWEEN TOTAL PLASMA HOMOCYSTEINE LEVEL AND GRADING OF CORONARY ARTERY DISEASE

    S. J. Mirhoseini

    2008-05-01

    Full Text Available Elevated total plasma homocysteine (tHcy levels constitute a risk factor for coronary artery disease (CAD. A possible relationship was investigated between admission plasma homocysteine level and the angiographic severity and extension of coronary artery disease in patients with CAD. This study looks at the relationship between total plasma homocysteine and severity of coronary artery disease. From April 2006 to December 2006, 100 consecutive patients (65 male and 35 female that referred to our institute for coronary artery bypass graft surgery enrolled. Fasting blood samples for homocysteine were obtained on admission. Plasma homocysteine concentration was measured with high-performance liquid chromatography (HPLC. Our patients presented in Group 1, total plasma homocysteine >12 micromoles per liter and Group 2, total plasma homocysteine =<12 micromoles per liter. Vessel score assessed the number of vessels with significant stenosis and grading of atherosclerosis (Extent Score was intended to assess the atherosclerotic involvement of the entire arterial length and circumscribe. Our study was shown age > 60 years was correlated with high tHcy, but gender, hypertension, history of smoking, hypercholesterolemia, family history, and diabetes mellitus were not statistically difference between two groups. A positive correlation was found between abnormal plasma homocysteine level and vessel score (r = 0.35; p=0.002. Moreover, a positive correlation was also found with extent score (r = 0.46; p =0.002. As results of these scoring, there was a better correlation between the tHcy level and the extent of CAD when compared with the vessel score (r = 0.68, p < 0.001. Abnormal elevated homocysteine levels in patients with coronary artery disease correlated with the extent of atherosclerotic disease.

  9. Apolipoprotein E gene polymorphism and total serum cholesterol level in Iranian population

    Bazzaz J

    2010-01-01

    Full Text Available Background: Apolipoprotein E (APOE is known as a major regulator of blood lipid levels in humans. A number of APOE gene allelic variants have been reported including E2, E3 and E4. Recent studies suggested a role for APOE in obesity and increased Body Mass Index (BMI and plasma lipid levels in obese children. Aim: The aim of this study was to examine the association between APOE genetic variants and the BMI and lipid profile in an Iranian cohort. Setting and Design: Samples were obtained from subjects who participated in a study based on the WHO-designed MONICA (multinational monitoring of trends and determinants in cardiovascular disease study for coronary artery disease risk assessment in Zone 17 of Tehran. The study was approved by the local ethical committee. Informed consent was obtained from all subjects included in this study. Materials and Methods: Subjects (n=320 were recruited. The level of triglyceride (TG and total serum cholesterol was tested for all subjects in this study. Genotyping for APOE was carried using polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLPtechnique. Statistical Analysis: Levels of significance were determined using contingency tables by either Chi-square or Fisher exact analysis using the STATA (v8 software. The analysis of regression and significance of differences for level of cholesterol and TG was established by one-way analysis of variance followed by Dunnett post hoc multiple comparison tests using SPSS software Version 11.5. Results: The frequency of allele E2 was significantly higher in patients with total serum cholesterol level <200 mg/dl (P 0.01 OR 2.1 95% CI 1.1-4.2. Conclusion: The association found in this study between allele E2 and lower total cholesterol level had been reported in previous studies. We have also observed that the frequency of genotype E2/E3 and E2/E4 was significantly higher in patients with normal total serum cholesterol level compared to patients with

  10. A low level of serum total testosterone is independently associated with nonalcoholic fatty liver disease

    Kim Sunmi

    2012-06-01

    Full Text Available Abstract Background The association between low serum testosterone levels, visceral adipose tissue (VAT, and metabolic syndrome is now well known. However, the relationship between hepatic steatosis and serum testosterone levels has not been extensively studied. Our aim was to investigate the association of serum total testosterone levels with nonalcoholic fatty liver disease (NAFLD, adjusting for the influence of VAT and insulin resistance. Methods This study is a retrospective observational cross-sectional one of healthy Korean men and was conducted at the Seoul National University Hospital Healthcare System Gangnam Center. We used data obtained from 495 men who were at least 20 years of age and who had undergone blood testing, abdominal computed tomography, and ultrasonography. Multiple logistic regression analysis was used to explore the association of serum total testosterone levels with NAFLD. Results Men in the low serum testosterone quintile were at a higher risk for NAFLD than men in the highest serum testosterone quintile. After adjusting for age, smoking, diabetes, exercise, BMI, triglycerides, and high-density-lipoprotein cholesterol, subjects with serum testosterone levels in the lowest quintile had an odds ratio (OR (95% confidence interval (CI of 5.12 (2.43–10.77 for NAFLD (p value, 0.0004. The inverse association between serum testosterone and NAFLD was attenuated by further adjustment for variables including VAT; however, it remained statistically significant (OR (95% CI: 4.52 (2.09–9.80 in the lowest quintile; p value=0.004. Conclusions A low serum total testosterone level was independently associated with NAFLD. This report is the first one suggesting the association remains unchanged even after controlling for VAT and insulin resistance.

  11. Total Jamur, Jenis Kapang dan Khamir Pellet Ayam Kampung Super dengan Penambahan Berbagai Level Pollard Berprobiotik

    Muhammad Nurdianto

    2015-10-01

    Full Text Available (Total fungi, type of mold and yeasts in super native chicken pelleted feed with various level of probiotic pollard ABSTRACT. The research objective is assessing the effect of adding various level of probiotic pollard on total fungi, type of mold and  yeast. The material used in this research were fermented vegetables waste , molasses, distilled water, pollard,  super native chicken’s feed, physiological NaCl (0.85% NaCl and sabaroud glucose agar (SGA.  Research using  completely randomized design with 4 treatments and 4 replications. The treatment used were T0 = 100% feed + 0% probiotic pollard, T1 = 90% feed + 10% probiotic pollard, T2 = 80% feed + 20% probiotic pollard and T3 = 70% feed + 30% probiotic pollard. The observed parameters were total fungi, type of molds and yeast. The average of total fungi are 0  CFU; 0,55 x 107 CFU; 0,55 x 107 CFU and 0 CFU. Type of mold is Aspergillus niger and none yeast have grown. The conclusion is the addition of 10% and 20%  probiotic pollard to super native chicken’s pellet  yield mold type Aspergillus niger as much 0,55 x 107 CFU.

  12. Adult total wellness: group differences based on sitting time and physical activity level

    Barwais, Faisal A; Cuddihy, Thomas F.; Tomson, L Michaud

    2014-01-01

    Background An increasing body of evidence associates a high level of sitting time with poor health outcomes. The benefits of moderate to vigorous-intensity physical activities to various aspects of health are now well documented; however, individuals may engage in moderate-intensity physical activity for at least 30 minutes on five or more days of the week and still exhibit a high level of sitting time. This purpose of this study was to examine differences in total wellness among adults relat...

  13. Total Quantum Zeno effect and Intelligent States for a two level system in a squeezed bath

    Mundarain, D; Stephany, J

    2006-01-01

    In this work we show that by frequent measurements of adequately chosen observables, a complete suppression of the decay in an exponentially decaying two level system interacting with a squeezed bath is obtained. The observables for which the effect is observed depend on the the squeezing parameters of the bath. The initial states which display Total Zeno Effect are intelligent states of two conjugate observables associated to the electromagnetic fluctuations of the bath.

  14. Evaluation of Salivary Level of Paraoxonase and Total Antioxidant Capacity in Type II Diabetic Subjects

    Sh. Radi; H.R. Abdolsamadi; Ranjbar, A.; N. Rafieian; Sh. Borzoei; Gh. Roshanaei; M. Jazyeri; F. Montazeran

    2015-01-01

    Introduction & Objective: Salivary antioxidants play important roles in the defensive mechanism of saliva against free radicals. Due to Prevalence of diabetes and numerous factors associated in its pathogenesis, understanding the defensive mechanism of salivary antioxidant against free radicals can provide helpful strategies in diagnosis and evaluation of diabetes. The aim of this study was evaluation of the paraoxonase (PON1) levels and total antioxidant capacity (TAC) of saliva in diabetic ...

  15. Total Antioxidant Level in Caffeine-free Herbal Infusions Suitable for Unrestricted Everyday Use

    Ruskovska, Tatjana; Gjorgieva, Darinka; Crcoroska, Marija; Kukovska, Valentina

    2011-01-01

    INTRODUCTION: Coffee and tea (Camellia sinensis), are widely used beverages among general population. АIM OF THE STUDY: Although there is no established recommended daily allowance for total antioxidants in human diet, we investigated alternative sources of antioxidants from caffeine-free and nonalcoholic beverages, free from preservatives, additives and artificial colors. CONCLUSION: In accordance with data from literature, green tea is superior with its antioxidants level.In respec...

  16. Carnitine levels in skeletal muscle of malnourished patients before and after total parenteral nutrition.

    Sandstedt, S; Larsson, J; Cederblad, G

    1986-11-01

    Carnitine is necessary for the transport of long-chain fatty acids across the mitochondrial membrane. Carnitine is derived from the diet and from endogenous synthesis from lysine and methionine. About 98% of the body's carnitine pool is located in skeletal muscle tissue. Skeletal muscle carnitine levels were determined in two groups of malnourished patients, eight patients with anorexia nervosa with a weight loss of 32.4% +/- 1.8 (mean +/- SEM) and six surgical patients with major gastrointestinal disorders and a weight loss of 15.2% +/- 2.7. Their hepatic and kidney functions were normal. On admission, the muscle carnitine levels were 16.9 +/- 4.0 mumol/g dry weight (mean +/- SD) for the surgical patients and 20.8 +/- 5.0 mumol/g dry weight for the anorexia nervosa patients, which corresponded to carnitine levels seen in healthy subjects. No statistical significance was found between the two groups. Total parenteral nutrition was given to the surgical patients for 2 weeks and to the anorexia nervosa patients for 3-5 weeks. No statistical difference in muscle carnitine levels was found in either group after nutritional support. These malnourished patients had no decreased muscle carnitine levels on admission and maintained them during several weeks of total parenteral nutrition. PMID:16831776

  17. Serum iron level, ferritin and total iron binding capacity level among nonpregnant women with and without melasma

    Elham Behrangi

    2015-01-01

    Full Text Available Background: Melasma is a common acquired disorder characterized by symmetric, hyperpigmented patches with an irregular outline, occurring most commonly on the face. It is most prevalent among young to middle-aged women. Although iron overload affects skin pigmentation, effect of iron deficiency on skin is not clear. So, we evaluated serum iron level, ferritin and total iron binding capacity (TIBC level among nonpregnant women with and without melasma. Materials and Methods: A cross-sectional case study was conducted in 2012 at university dermatologic department on 33 nonpregnant women with melasma (case and 33 nonpregnant women without melasma (control. Serum iron level, TIBC and ferritin in the two groups was measured and compared. Results: Serum iron level was lower in the case group (85 ± 11 in comparison with control group (102 ± 9, but the difference was not significant (P: 0.9. Mean TIBC and Ferritin were higher in the case group (TIBC: 329.4 ± 29, ferritin: 6 ± 18 than the control group (TIBC: 329.3 ± 29, ferritin: 33 ± 6 without significant difference. Conclusion: Although the serum iron level was lower in nonpregnant women with mealsma, it was not significant compared with those without melasma.

  18. An investigation into the effectiveness of sand media amended with biochar to remove BOD5, suspended solids and coliforms using wetland mesocosms.

    de Rozari, P; Greenway, M; El Hanandeh, A

    2015-01-01

    Constructed wetland ecotechnologies (CWEs) are a promising solution to effectively treat domestic wastewater in developing countries at low cost. This paper reports the findings of the effectiveness of sand media amended with woody biochar and two plants species (Melaleuca quinquenervia and Cymbopogon citratus) in removing biological oxygen demand (BOD5), suspended solids and coliforms. The experimental design consisted of 21 vertical flow (VF) mesocosms. There were seven media treatments using sand amended with varying proportions of biochar. During the first 8 months, the mesocosms were loaded with secondary clarified wastewater (SCW) then septage. The influent had a 4-day hydraulic retention time. Samples were monitored for BOD5, total suspended solids (TSS), total volatile solids (TVS), total coliforms and faecal coliforms. In the first 8 months, there were no significant performance differences between media treatments in the outflow concentrations of BOD5, TSS and TVS. The significant differences occurred during the last 3 months; using septage with biochar additions performed better than pure sand. For coliforms, the significant differences occurred after 6 months. In conclusion, the addition of biochar was not effective for SCW. The VF mesocosms system proved to be more effective in removing BOD5, TSS, TVS and coliforms when septage was loaded into the media. PMID:26442496

  19. Annoyance from Road Traffic, Trains, Airplanes and from Total Environmental Noise Levels.

    Ragettli, Martina S; Goudreau, Sophie; Plante, Céline; Perron, Stéphane; Fournier, Michel; Smargiassi, Audrey

    2016-01-01

    There is a lack of studies assessing the exposure-response relationship between transportation noise and annoyance in North America. Our aims were to investigate the prevalence of noise annoyance induced by road traffic, trains and airplanes in relation to distance to transportation noise sources, and to total environmental noise levels in Montreal, Canada; annoyance was assessed as noise-induced disturbance. A telephone-based survey among 4336 persons aged >18 years was conducted. Exposure to total environmental noise (A-weighted outdoor noise levels-LAeq24h and day-evening-night equivalent noise levels-Lden) for each study participant was determined using a statistical noise model (land use regression-LUR) that is based on actual outdoor noise measurements. The proportion of the population annoyed by road traffic, airplane and train noise was 20.1%, 13.0% and 6.1%, respectively. As the distance to major roads, railways and the Montreal International Airport increased, the percentage of people disturbed and highly disturbed due to the corresponding traffic noise significantly decreased. When applying the statistical noise model we found a relationship between noise levels and disturbance from road traffic and total environmental noise, with Prevalence Proportion Ratios (PPR) for highly disturbed people of 1.10 (95% CI: 1.07-1.13) and 1.04 (1.02-1.06) per 1 dB(A) Lden, respectively. Our study provides the first comprehensive information on the relationship between transportation noise levels and disturbance in a Canadian city. LUR models are still in development and further studies on transportation noise induced annoyance are consequently needed, especially for sources other than road traffic. PMID:26729143

  20. Total Testosterone Levels and the Effect of Sildenafil on Type 2 Diabetics with Erectile Dysfunction

    Nabeel Najib Fadhil Hadeed

    2014-01-01

    Full Text Available Objectives: Hypotestosteronemia has been reported in approximately half of type 2 diabetic men in general. This study aims to assess serum total testosterone levels in type 2 diabetics with erectile dysfunction and to correlate the degree of improvement between sildenafil citrate and testosterone levels. Methods: A cross sectional and prospective comparative interventional study was conducted at the Diabetic Clinic of Assalam Teaching Hospital in Mosul, during the period from January 1, 2009 through to December 31, 2011. The study enrolled 120 type 2 diabetic males with erectile dysfunction who were analyzed with regard to age, duration of diabetes, duration and severity of erectile dysfunction, serum total testosteron levels and the degree of response to sildenafil citrate in terms of testosterone levels. The data were statistically analyzed using the independent two-sample Student t test, χ2 test and Pearson correlation test. A p-value of <0.05 was considered statistically significant. Results: Thirty six percent of type 2 diabetic males with erectile dysfunction were found to have low serum testosterone levels. The hypotestosteronemic and normotestosteronemic subgroups were not significantly different in terms of mean age, duration of diabetes, reduction of libido, and reduction in erectile function. The rate and the degree of improvement of erection by sildenafil in the normo-and-hypotestosteronemic respondents were not significantly different, but the degree of improvement by sildenafil was significantly correlated to testosterone levels among the hypotestosteronemic group. Conclusion: Hypotestosteronemia was found in 36% of type 2 diabetic males with erectile dysfunction. The degree of improvement of erectile dysfunction by sildenafil was directly proportional to the serum testosterone levels among the hypotestosteronemic group. Therapeutic supplement with testosterone preparation in the hypotestosteronemic diabetics with erectile

  1. 40 CFR 141.802 - Coliform sampling plan.

    2010-07-01

    ... sampling plan for each aircraft with a water system meeting the definition of a public water system by April 19, 2011. (c) The coliform sampling plan must be included in the Aircraft Water System Operations... aircraft water system owned or operated by the air carrier that identifies the following: (1)...

  2. Office workers' objectively assessed total and prolonged sitting time: Individual-level correlates and worksite variations.

    Hadgraft, Nyssa T; Healy, Genevieve N; Owen, Neville; Winkler, Elisabeth A H; Lynch, Brigid M; Sethi, Parneet; Eakin, Elizabeth G; Moodie, Marj; LaMontagne, Anthony D; Wiesner, Glen; Willenberg, Lisa; Dunstan, David W

    2016-12-01

    Sedentary behavior is highly prevalent in office-based workplaces; however, few studies have assessed the attributes associated with this health risk factor in the workplace setting. This study aimed to identify the correlates of office workers' objectively-assessed total and prolonged (≥ 30 min bouts) workplace sitting time. Participants were 231 Australian office workers recruited from 14 sites of a single government employer in 2012-13. Potential socio-demographic, work-related, health-related and cognitive-social correlates were measured through a self-administered survey and anthropometric measurements. Associations with total and prolonged workplace sitting time (measured with the activPAL3) were tested using linear mixed models. Worksites varied significantly in total workplace sitting time (overall mean [SD]: 79% [10%] of work hours) and prolonged workplace sitting time (42% [19%]), after adjusting for socio-demographic and work-related characteristics. Organisational tenure of 3-5 years (compared to tenure > 5 years) was associated with more time spent in total and prolonged workplace sitting time, while having a BMI categorised as obese (compared to a healthy BMI) was associated with less time spent in total and prolonged workplace sitting time. Significant variations in sitting time were observed across different worksites of the same employer and the variation remained after adjusting for individual-level factors. Only BMI and organisational tenure were identified as correlates of total and prolonged workplace sitting time. Additional studies are needed to confirm the present findings across diverse organisations and occupations. PMID:27413681

  3. Determination of total cyanide in Hanford Site high-level wastes

    Winters, W.I. [Westinghouse Hanford Co., Richland, WA (United States); Pool, K.H. [Pacific Northwest Lab., Richland, WA (United States)

    1994-05-01

    Nickel ferrocyanide compounds (Na{sub 2-x}Cs{sub x}NiFe (CN){sub 6}) were produced in a scavenging process to remove {sup 137}Cs from Hanford Site single-shell tank waste supernates. Methods for determining total cyanide in Hanford Site high-level wastes are needed for the evaluation of potential exothermic reactions between cyanide and oxidizers such as nitrate and for safe storage, processing, and management of the wastes in compliance with regulatory requirements. Hanford Site laboratory experience in determining cyanide in high-level wastes is summarized. Modifications were made to standard cyanide methods to permit improved handling of high-level waste samples and to eliminate interferences found in Hanford Site waste matrices. Interferences and associated procedure modifications caused by high nitrates/nitrite concentrations, insoluble nickel ferrocyanides, and organic complexants are described.

  4. Determination of total cyanide in Hanford Site high-level wastes

    Nickel ferrocyanide compounds (Na2-xCsxNiFe (CN)6) were produced in a scavenging process to remove 137Cs from Hanford Site single-shell tank waste supernates. Methods for determining total cyanide in Hanford Site high-level wastes are needed for the evaluation of potential exothermic reactions between cyanide and oxidizers such as nitrate and for safe storage, processing, and management of the wastes in compliance with regulatory requirements. Hanford Site laboratory experience in determining cyanide in high-level wastes is summarized. Modifications were made to standard cyanide methods to permit improved handling of high-level waste samples and to eliminate interferences found in Hanford Site waste matrices. Interferences and associated procedure modifications caused by high nitrates/nitrite concentrations, insoluble nickel ferrocyanides, and organic complexants are described

  5. Modelling faecal coliform mortality in water hyacinths ponds

    Mayo, A. W.; Kalibbala, M.

    Removal of faecal coliforms was investigated in pilot-scale water hyacinths ponds. The investigation was conducted to evaluate the role of solar intensity, pH, dissolved oxygen, temperature, sedimentation, and attachment of faecal coliforms on Eichhornia crassipes on disappearance of bacteria in water hyacinths ponds. A mathematical model that used the plug flow philosophy and incorporating the aforementioned factors was developed to predict faecal coliform mortality rate. The proposed multifactor model satisfactorily predicted mortality rate of faecal coliforms in a pilot-scale water hyacinths ponds. After optimization of the parameters, mortality rate constant for pH ( kpH) was 0.001, mortality rate constant for DO ( kDO) was 0.0037 and solar intensity mortality rate constant k s was 0.0102 cm 2/cal. The results also showed that the thickness of biofilm ( Lf) was 2.5 × 10 -4 m, and the effective surface area of water hyacinths roots per unit surface area of pond ( Rs) was 10.4 m 2/m 2. The results further showed that environmental factors such as solar intensity and pH were the key factors when water hyacinths ponds have a large exposed surface area. However, attachment of bacteria to water hyacinths played a major role in ponds fully covered with water hyacinths. The inclusion of sedimentation parameters in the model improved model efficiency by only 3.2%. It was concluded that sedimentation is not a major factor governing faecal coliform disappearance in water hyacinths pond systems receiving pretreated wastewaters.

  6. Low-level waste disposal performance assessments - Total source-term analysis

    Wilhite, E.L.

    1995-12-31

    Disposal of low-level radioactive waste at Department of Energy (DOE) facilities is regulated by DOE. DOE Order 5820.2A establishes policies, guidelines, and minimum requirements for managing radioactive waste. Requirements for disposal of low-level waste emplaced after September 1988 include providing reasonable assurance of meeting stated performance objectives by completing a radiological performance assessment. Recently, the Defense Nuclear Facilities Safety Board issued Recommendation 94-2, {open_quotes}Conformance with Safety Standards at Department of Energy Low-Level Nuclear Waste and Disposal Sites.{close_quotes} One of the elements of the recommendation is that low-level waste performance assessments do not include the entire source term because low-level waste emplaced prior to September 1988, as well as other DOE sources of radioactivity in the ground, are excluded. DOE has developed and issued guidance for preliminary assessments of the impact of including the total source term in performance assessments. This paper will present issues resulting from the inclusion of all DOE sources of radioactivity in performance assessments of low-level waste disposal facilities.

  7. [Levels of Ochratoxin A and total Aflatoxins in Panamanian exportation coffee by an ELISA Method].

    Franco, Heriberto; Vega, Aracelly; Reyes, Stephany; De Léon, Javier; Bonilla, Alexis

    2014-03-01

    A study about processing conditions of exportation coffee in 15 benefits located in Chiriqui, western region of Panama, was conducted. In addition, 21 samples of processed coffee (green beans), from the benefits, were analyzed. The samples were microbiologically tested in order to quantify total aflatoxins (B1, B2, G1 and G2) and Ochratoxin A (OTA), using the immunoaffinity ELISA method. A detection limit of 0.017 ng/mL, was determined for Ochratoxin A, which is equivalent to a concentration of 0.829 µg/kg, and a detection limit of 0.027 ng/mL, for total aflatoxins, which is equivalent to a concentration of 1.350 µg/kg. It was found that four (19%) out of the 21 samples were positive to the presence of Ochratoxin A and three (14%) to the presence of total aflatoxins. Samples showed levels of Ochratoxin A in the range 4.90 - 37.73 µg/kg; only three of them exceeded the maximum limit allowed by the European Union, for the concentration of Ochratoxin, which is of 5.0 µg/kg. Total aflatoxins were found in the range 1.51 - 1.93 µg/kg, below 10 µg/kg which is the maximum limit allowed for coffee by the European Union. The results indicate that the processing of coffee produced in Panama successfully meets international standards for postharvest handling, which leads to a low incidence ofmycotoxins and very low levels ofmycotoxin-producing fungi. PMID:25796716

  8. Annoyance from Road Traffic, Trains, Airplanes and from Total Environmental Noise Levels

    Martina S. Ragettli

    2015-12-01

    Full Text Available There is a lack of studies assessing the exposure-response relationship between transportation noise and annoyance in North America. Our aims were to investigate the prevalence of noise annoyance induced by road traffic, trains and airplanes in relation to distance to transportation noise sources, and to total environmental noise levels in Montreal, Canada; annoyance was assessed as noise-induced disturbance. A telephone-based survey among 4336 persons aged >18 years was conducted. Exposure to total environmental noise (A-weighted outdoor noise levels—LAeq24h and day-evening-night equivalent noise levels—Lden for each study participant was determined using a statistical noise model (land use regression—LUR that is based on actual outdoor noise measurements. The proportion of the population annoyed by road traffic, airplane and train noise was 20.1%, 13.0% and 6.1%, respectively. As the distance to major roads, railways and the Montreal International Airport increased, the percentage of people disturbed and highly disturbed due to the corresponding traffic noise significantly decreased. When applying the statistical noise model we found a relationship between noise levels and disturbance from road traffic and total environmental noise, with Prevalence Proportion Ratios (PPR for highly disturbed people of 1.10 (95% CI: 1.07–1.13 and 1.04 (1.02–1.06 per 1 dB(A Lden, respectively. Our study provides the first comprehensive information on the relationship between transportation noise levels and disturbance in a Canadian city. LUR models are still in development and further studies on transportation noise induced annoyance are consequently needed, especially for sources other than road traffic.

  9. Nitric Oxide Levels and Total Antioxidant Capacity in The Seminal Plasma of Infertile Smoking Men

    Yousefreza Yousefniapasha

    2015-04-01

    Full Text Available Objective: Cigarette is a rich source of oxidants and reactive nitrogen species. Nitric oxide (NO in high concentration has deleterious effects on human sperm function. Antioxidant defense system in seminal plasma protects spermatozoa from the attack of reactive oxygen metabolites. The purpose of this study was to determine the correlation between cigarette smoking with the NO levels and the total antioxidant capacity (TAC of the seminal plasma in infertile smoker men and to compare severity of oxidative stress (OS in them with fertile and infertile non-smoking men. Materials and Methods: In this cross sectional study, a total of 95 male participants attended the Infertility Clinic of the Fatehmeh-zahra Hospital in Babol, Mazandaran Province, Iran, between 2010 and 2011. They were divided into three groups: I. fertile non-smokers (F.ns; n=32, II. infertile non-smokers (IF.ns; n=30 and III. infertile smokers (IF.s; n=33 according to semen analysis World Health Organization guidelines (WHO, 2001 and smoking data. TAC concentration and NO levels of seminal plasma were measured using the ferric reducing ability of plasma (FRAP method and the Griess reagent, respectively. Results: Standard sperm parameters were significantly higher in the fertile group than those in the infertile groups, but these differences between the IF.ns and IF.s were not statistically significant. The mean TAC in the seminal plasma was higher in the F.ns>IF.ns>IF.s, respectively, but these differences were not statistically significant among three groups (p= 0.096. In contrast, the mean NO level in the seminal plasma was lower in the F.nslevels associated with smoking might exceed the capacity of antioxidant defense system, leading to increased oxidative damage of seminal plasma and decreased fertility in men.

  10. Homocystein Level and Total Antioxidant Capacity in Chronic Obstructive Pulmonary Disease

    Shirakdehi, MS. (MSc

    2014-06-01

    Full Text Available Background and Objective: Oxidant-antioxidant imbalance plays a key role in pathogenesis of chronic obstructive pulmonary disease (COPD. This study aimed to evaluate homocystiene and total antioxidant capacity in COPD patients, compared to smoker and non-smoker healthy people. Material and Methods: We measured total antioxidant capacity with Cayman Kit, uric acid with Pars Azmoon kit٫ homocysteine with ELISA Kit and inflammatory cells (leukocytes in 29 COPD patients, 29 smokers and 29 non-smokers. Results: Uric acid was significantly higher in COPD patients compared to healthy smokers and healthy non-smokers (p<0.05. Total antioxidant capacity was significantly lower in COPD patients compared to healthy, non smokers (p=0. 003. In COPD patients, homocysteine and leukocytes levels were significantly higher than those in healthy smokers (P<0.05 and healthy non- smokers (p<0.001. Conclusion: According to high inflammatory cells and low antioxidant capacity in COPD, early administration of appropriate medication is recommended to reduce systemic and topical inflammation. Reduction in the exposure to oxidizing compounds can slow the process of degradation and damage to lungs. Keywords: Chronic Obstructive Pulmonary Disease; Homocysteine; Oxidative Stress

  11. Sampling and Pooling Methods for Capturing Herd Level Antibiotic Resistance in Swine Feces using qPCR and CFU Approaches

    Schmidt, Gunilla Veslemøy; Mellerup, Anders; Christiansen, Lasse Engbo;

    2015-01-01

    The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays...... for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic resistance genes in total DNA from swine fecal samples that were obtained using different sampling and pooling methods. In parallel, the number of antibiotic resistant coliform indicator bacteria was...... determined in the same swine fecal samples. The results showed that the qPCR assays were capable of detecting differences in antibiotic resistance levels in individual animals that the coliform bacteria colony forming units (CFU) could not. Also, the qPCR assays more accurately quantified antibiotic...

  12. Characteristics of High-density Lipoprotein Subclasses Distribution for Subjects with Desirable Total Cholesterol Levels

    Xu Yanhua

    2011-04-01

    Full Text Available Abstract Background To investigate alteration of high density lipoproteins (HDL subclasses distribution in different total cholesterol (TC levels, mainly the characteristics of HDL subclasses distribution in desirable TC levels and analyze the related mechanisms. Methods ApoA-I contents of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection. 486 Chinese Adults subjects were assigned to different TC groups according to the third Report of NCEP (ATP- III guidelines. Results The increase in contents of small preβ1-HDL, HDL3c, HDL3b, and HDL3a particles clustered and reduce in HDL2b with increased of TC. The distribution of HDL subclasses have shown abnormality characterized by the lower HDL2b (324.2 mg/L contents and the higher preβ1-HDL (90.4 mg/L contents for desirable TC Chinese subjects. Among 176 desirable TC subjects, 58.6% subjects with triglyceride (TG Conclusions The particles size of HDL subclasses shifted towards smaller with increased TC levels. The TC was liner with HDL2b contents and those can be reduced 17 mg/L for 0.5 mmol/L increment in TC levels. The HDL subclasses distribution phenotype was not expectation for Chinese Population with desirable TC levels. Thus, from the HDL subclasses distribution point, when assessing the coronary heart disease(CHD risk not only rely on the TC levels, but also the concentrations of TG, HDL-C and LDL-C must considered in case the potential risk for desirable TC subjects with other plasma lipids metabolism disorders.

  13. Controlled disposal of domestic effluent sewage in the ground to reduce fecal coliforms

    Paulo Fortes Neto

    2008-12-01

    Full Text Available The indiscriminate launching in water bodies of domestic sewage without treatment, or even treated, but without appropriate disinfection, contributes with significant amount of organisms of the called "coliform group” that can carry specific illnesses agents propagated through the water. The application of effluent in the ground, instead of direct disposal in water courses, in addition to being an alternative way for the disposal of residues and biological control of pollutants, constitutes an adequate way of nutrients supply to the soil and plants. So, this work had as objective the evaluation of the reduction of fecal coliforms, after controlled applications of 60 days treated effluent in cultivated soil, by analyzing the increase of fluorescent rhizobacterias Pseudomonas fluorescens and Bacillus spp. present in the rhizospheres of different crops. The experiment was developed in field conditions in the Experimental Farm of Department of Agrarian Sciences of the University of Taubaté, municipality of Taubaté, SP. The Experimental design consisted of random blocks, with five treatments including annual crops (Oats, Barley, Triticale - a cross between wheat and rye, Black Beans and non-cultivated soil as witness - blank reference and four repetitions, totalizing 20 ground plots with area of 2 m x 1 m with 50 cm space among plots on a Dystrophic Red-Yellow Latossol. Results from the microbial analyses of rhizosphere and non-rhizosphere soil indicated that the rhizosphere of oats had denser rhizobacterias than the other crops. However, the greatest efficiency was found in the reduction of thermo-tolerant coliforms for both black beans and non-cultivated soil.

  14. Leveling Process of Total Electron Content (TEC Using Malaysian Global Positioning System (GPS Data

    Y. Norsuzila

    2008-01-01

    Full Text Available The signals from the satellites of the Navstar Global Positioning System (GPS travel through the earth's ionosphere on their way to GPS receivers. However, ionospheric delay is one of the main sources of error in GPS. The magnitude of the ionospheric delay is influenced by the Total Electron Content (TEC along the radio wave path from a GPS satellite to the ground receiver. This study investigates the TEC using GPS data collected from Wisma Tanah, Kuala Lumpur, KTPK (3° 10' 15.44"N, 101° 43' 03.35"E station and processed and analyzed under quiet geomagnetic day at the equatorial region on 8 November 2005. This research assesses the errors translated from the code-delay to the carrier-phase ionospheric observable by the so-called leveling process, which was applied to reduce multipath from the data. It was found that the leveled carrier-phase ionosphere observable was affected by a systematic error, produced by code-delay multipath through the leveling procedure. The effects, however, do not cancel after averaging all the data. Dual frequency carrier-phase and code-delay GPS observations are combined to obtain ionospheric observable related to the slant TEC (TECs along the satellite-receiver line of sight (los. This results in the absolute differential delay and the remaining noise was discarded. These are the first results obtained using TEC-GPS technique for TEC measurement in Malaysia.

  15. Breakdown of low-level total petroleum hydrocarbons (TPH) in contaminated soil using grasses and willows.

    McIntosh, Patrick; Kuzovkina, Yulia A; Schulthess, Cristian P; Guillard, Karl

    2016-01-01

    A phytoremediation study targeting low-level total petroleum hydrocarbons (TPH) was conducted using cool- and warm-season grasses and willows (Salix species) grown in pots filled with contaminated sandy soil from the New Haven Rail Yard, CT. Efficiencies of the TPH degradation were assessed in a 90-day experiment using 20-8.7-16.6 N-P-K water-soluble fertilizer and fertilizer with molasses amendments to enhance phytoremediation. Plant biomass, TPH concentrations, and indigenous microbes quantified with colony-forming units (CFU), were assessed at the end of the study. Switchgrass grown with soil amendments produced the highest aboveground biomass. Bacterial CFU's were in orders of magnitude significantly higher in willows with soil amendments compared to vegetated treatments with no amendments. The greatest reduction in TPH occurred in all vegetated treatments with fertilizer (66-75%) and fertilizer/molasses (65-74%), followed sequentially by vegetated treatments without amendments, unvegetated treatments with amendments, and unvegetated treatments with no amendment. Phytoremediation of low-level TPH contamination was most efficient where fertilization was in combination with plant species. The same level of remediation was achievable through the addition of grasses and/or willow combinations without amendment, or by fertilization of sandy soil. PMID:26553847

  16. SORCE Level 3 Total Solar Irradiance 6-Hour Average V016

    National Aeronautics and Space Administration — The Total Solar Irradiance (TSI) data set SOR3TSI6 contains the total solar irradiance (a.k.a solar constant) data collected by the Total Irradiance Monitor (TIM)...

  17. Survival of fecal coliforms in dry-composting toilets.

    Redlinger, T; Graham, J; Corella-Barud, V; Avitia, R

    2001-09-01

    The dry-composting toilet, which uses neither water nor sewage infrastructure, is a practical solution in areas with inadequate sewage disposal and where water is limited. These systems are becoming increasingly popular and are promoted to sanitize human excreta and to recycle them into fertilizer for nonedible plants, yet there are few data on the safety of this technology. This study analyzed fecal coliform reduction in approximately 90 prefabricated, dry-composting toilets (Sistema Integral de Reciclamiento de Desechos Orgánicos [SIRDOs]) that were installed on the U.S.-Mexico border in Ciudad Juárez, Chihuahua, Mexico. The purpose of this study was to determine fecal coliform reduction over time and the most probable method of this reduction. Biosolid waste samples were collected and analyzed at approximately 3 and 6 months and were classified based on U.S. Environmental Protection Agency standards. Results showed that class A compost (high grade) was present in only 35.8% of SIRDOs after 6 months. The primary mechanism for fecal coliform reduction was found to be desiccation rather than biodegradation. There was a significant correlation (P = 0.008) between classification rating and percent moisture categories of the biosolid samples: drier samples had a greater proportion of class A samples. Solar exposure was critical for maximal class A biosolid end products (P = 0.001). This study only addressed fecal coliforms as an indicator organism, and further research is necessary to determine the safety of composting toilets with respect to other pathogenic microorganisms, some of which are more resistant to desiccation. PMID:11526002

  18. Evaluation of Salivary Level of Paraoxonase and Total Antioxidant Capacity in Type II Diabetic Subjects

    Sh. Radi

    2015-07-01

    Full Text Available Introduction & Objective: Salivary antioxidants play important roles in the defensive mechanism of saliva against free radicals. Due to Prevalence of diabetes and numerous factors associated in its pathogenesis, understanding the defensive mechanism of salivary antioxidant against free radicals can provide helpful strategies in diagnosis and evaluation of diabetes. The aim of this study was evaluation of the paraoxonase (PON1 levels and total antioxidant capacity (TAC of saliva in diabetic subjects. Materials & Methods: In this case -control study, 40 patients diagnosed with type II diabetes (20 males and 20 females aged between 40 to 60 years and 40 healthy controls were selected. 5 mL unstimulated saliva samples using Spiting method were collected. PON-I and TAC levels were examined using FRAP (Ferric Reducing Ability of Plasma methods. The data were ana-lyzed by t- test or its non parametric equivalent tests. Results: Mean PON-I in the subjects and control group were 8.05±2.099 nmol /ml 9.98 ±2.957 nmol/ml, respectively and the difference was significant (P=0.001 TAC in saliva of the sub-jects and control group were 377.38 ±191.229 and 402.25±189.105 and no significant differ-ence was found (P=0.56. Conclusion: The level of salivary TAC, PON-1 of patients with type II diabetes was lower than healthy controls. Additionally, plasma low density lipoproteins level of the diabetic subjects was higher compared with the healthy ones.(Sci J Hamadan Univ Med Sci 2015; 22 (2: 114-121

  19. Determination and assessment of total mercury levels in local, frozen and canned fish in Lebanon

    Pierre J Obeid; Bilal E1-Khoury; Joanne Burger; Samer Aouad; Mira Younis; Amal Aoun; John Hanna El-Nakat

    2011-01-01

    Fish is an important constituent of the Lebanese diet.However,very little attention in our area is given to bring awareness regarding the effect of the toxicity of mercury (Hg) mainly through fish consumption.This study aimed to report analytical data on total mercury levels in several fish species for the first time in thirty years and to also made individuals aware of the presence and danger from exposure to mercury through fish consumption.Fish samples were selected from local Lebanese markets and fisheries and included 94 samples of which were fresh,frozen,processed,and canned fish.All values were reported as microgram of mercury per gram of fish based on wet weight.The level of mercury ranged from 0.0190 to 0.5700 μg/g in fresh samples,0.0059 to 0.0665 μg/g in frozen samples,and 0.0305 to 0.1190 μg/g in canned samples.The data clearly showed that higher levels of mercury were detected in local fresh fish as opposed to other types thus placing consumers at higher risk from mercury exposure.Moreover,the data revealed that Mallifa (yellowstripe barracuda/Sphyraena chrysotaenia),Sargous (white seabream/Diplodus sargus),Ghobbos (bogue/Boops boops),and shrimp (Penaeus sp.) were among the types containing the highest amounts of mercury.On the other hand,processed fish such as fish fillet,fish burger,small shrimp and crab are found to contain lower levels of mercury and are associated with lower exposure risks to mercury.Lebanese population should therefore,be aware to consume limited amounts of fresh local fish to minimize exposure to mercury.

  20. Survey of PAC Performance for Removal of Turbidity, COD, Coliform Bacteria, Heterotrophic Bacteria from Water of Karoon River

    N Alavi Bakhtiarvand

    2011-10-01

    Full Text Available Backgrounds and Objectives: Selection of proper coagulants for turbidity removal and determination of effective methods to reduce coagulants dose and related costs in water treatment plants is of critical importance. The present study investigates the effect of returned sludge on improving the performance of poly-aluminum chloride (PAC in turbidity, coliform bacteria, heterotrophic bacteria removal from drinking water during rapid mixing phase. Materials and Methods: In order to determine the optimal returned sludge volume injected during rapid mixing with PAC for turbidity, total coliform and hetrophic bacteria, experiments were conducted based on variables such as injected silt volume (from 0 - 125 ml, and varying turbidities from 58 - 112 NTU. At the end of each JAR experiments, remaining turbidity , microbial parameters of samples were measured . Coagulant efficiency in turbidity removal and microbial parameters were determined by Covariance, Duncan analyses and graphs were drawn by MS Excel . The results statistically showed significant among variables (P<0.05. Results: The results showed that the maximum turbidity removal efficiency of 98.92 at 30 ppm was 10 ml while the maximum turbidity removal efficiency of 98.31 at 10 ppm was 4 ml. The maximum total coliform removal efficiency  of 95.68 obtained for 10 ppm in 10 cc injected sludge volume.Conclusion: This study shows that addition of returned sludge to flash mixing can reduce the turbidity of samples.

  1. Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture (Most Probably Number

    Dehghan fatemeh

    2014-05-01

    Conclusions: Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city. It's recommended to be used at least as an initial screening test, and then the positive samples could be randomly tested by MPN.

  2. Fate of coliforms and pathogenic parasite in four full-scale sewage treatment systems in India.

    Tyagi, Vinay Kumar; Sahoo, B K; Khursheed, Anwar; Kazmi, A A; Ahmad, Z; Chopra, A K

    2011-10-01

    The occurrence and removal of fecal indicators (total coliforms (TC), fecal coliforms (FC), fecal streptococci (FS)) and pathogens (helminthes eggs) were studied in various municipal wastewater treatment processes (UASB + FPU, ASP, EA, WSP). The reductions in TC and FC concentrations were usually between 2.0 and 2.5 log units in up-flow anaerobic sludge blanket reactor incorporated with final polishing unit (UASB + FPU). Almost similar reduction was observed in activated sludge process system (ASP) and waste stabilization ponds system (WSP), while it was log 3.0 in extended aeration system (EA). UASB + FPU and WSP systems were observed more efficient to reduce helminthes eggs at almost 100%, whereas only 97% removal was observed in case of ASP and EA system. In addition to monitoring of indicator organisms, turbidity, suspended solids (SS), and biochemical oxygen demand (BOD) were used as indirect measure of the potential presence of microorganisms. Interrelationship of BOD, SS, and turbidity with fecal indicator bacteria concentration in influent and effluent manifest that improvement of the microbiological quality of wastewater is strongly linked to the removal of BOD and SS. PMID:21136285

  3. Sleep Disturbance from Road Traffic, Railways, Airplanes and from Total Environmental Noise Levels in Montreal.

    Perron, Stéphane; Plante, Céline; Ragettli, Martina S; Kaiser, David J; Goudreau, Sophie; Smargiassi, Audrey

    2016-01-01

    The objective of our study was to measure the impact of transportation-related noise and total environmental noise on sleep disturbance for the residents of Montreal, Canada. A telephone-based survey on noise-related sleep disturbance among 4336 persons aged 18 years and over was conducted. LNight for each study participant was estimated using a land use regression (LUR) model. Distance of the respondent's residence to the nearest transportation noise source was also used as an indicator of noise exposure. The proportion of the population whose sleep was disturbed by outdoor environmental noise in the past 4 weeks was 12.4%. The proportion of those affected by road traffic, airplane and railway noise was 4.2%, 1.5% and 1.1%, respectively. We observed an increased prevalence in sleep disturbance for those exposed to both rail and road noise when compared for those exposed to road only. We did not observe an increased prevalence in sleep disturbance for those that were both exposed to road and planes when compared to those exposed to road or planes only. We developed regression models to assess the marginal proportion of sleep disturbance as a function of estimated LNight and distance to transportation noise sources. In our models, sleep disturbance increased with proximity to transportation noise sources (railway, airplane and road traffic) and with increasing LNight values. Our study provides a quantitative estimate of the association between total environmental noise levels estimated using an LUR model and sleep disturbance from transportation noise. PMID:27529260

  4. Sleep Disturbance from Road Traffic, Railways, Airplanes and from Total Environmental Noise Levels in Montreal

    Stéphane Perron

    2016-08-01

    Full Text Available The objective of our study was to measure the impact of transportation-related noise and total environmental noise on sleep disturbance for the residents of Montreal, Canada. A telephone-based survey on noise-related sleep disturbance among 4336 persons aged 18 years and over was conducted. LNight for each study participant was estimated using a land use regression (LUR model. Distance of the respondent’s residence to the nearest transportation noise source was also used as an indicator of noise exposure. The proportion of the population whose sleep was disturbed by outdoor environmental noise in the past 4 weeks was 12.4%. The proportion of those affected by road traffic, airplane and railway noise was 4.2%, 1.5% and 1.1%, respectively. We observed an increased prevalence in sleep disturbance for those exposed to both rail and road noise when compared for those exposed to road only. We did not observe an increased prevalence in sleep disturbance for those that were both exposed to road and planes when compared to those exposed to road or planes only. We developed regression models to assess the marginal proportion of sleep disturbance as a function of estimated LNight and distance to transportation noise sources. In our models, sleep disturbance increased with proximity to transportation noise sources (railway, airplane and road traffic and with increasing LNight values. Our study provides a quantitative estimate of the association between total environmental noise levels estimated using an LUR model and sleep disturbance from transportation noise.

  5. Matrix Extension Study: Validation of the Compact Dry EC Method for Enumeration of Escherichia coli and non-E. coli Coliform Bacteria in Selected Foods.

    Mizuochi, Shingo; Nelson, Maria; Baylis, Chris; Green, Becky; Jewell, Keith; Monadjemi, Farinaz; Chen, Yi; Salfinger, Yvonne; Fernandez, Maria Cristina

    2016-01-01

    The Compact Dry "Nissui" EC method, originally certified by the AOAC Research Institute Performance Test Method(SM) program for enumeration of Escherichia coli and non-E. coli coliforms in raw meat products (Performance Tested Method(SM) 110402), has undergone an evaluation to extend the method's claim to cooked chicken, prewashed bagged shredded iceberg lettuce, frozen cod filets, instant nonfat dry milk powder, and pasteurized milk (2% fat). Compact Dry EC is a ready-to-use dry media sheet containing a cold-soluble gelling agent, selective agents, and a chromogenic medium, which are rehydrated by adding 1 mL diluted sample. E. coli form blue/blue-purple colonies, whereas other coliform bacteria form red/pink colonies. Users can obtain an E. coli count (blue/blue-purple colonies only) and a total coliform count (red/pink plus blue/blue-purple colonies) after 24 ± 2 h of incubation at 37 ± 1°C. The matrix extension study was organized by Campden BRI (formerly Campden and Chorleywood Food Research Association Technology, Ltd), Chipping Campden, United Kingdom. Method comparison data for cooked chicken, prewashed bagged shredded iceberg lettuce, frozen cod filets, and instant nonfat dry milk powder were collected in a single-laboratory evaluation by Campden BRI. A multilaboratory study was conducted on pasteurized milk (2% fat), with 13 laboratories participating. The Compact Dry EC method was compared to ISO 16649-2:2001 "Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli-Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide" and to ISO 4832:2006 "Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coliforms-Colony-count technique," the current standards at the time of this study. Each matrix was evaluated separately for E. coli and non-E. coli coliforms at each contamination level (including an

  6. THE ASSOCIATION BETWEEN G6PD DEFICIENCY AND TOTAL SERUM BILIRUBIN LEVEL IN ICTERIC NEONATES

    S. Behjati-Ardakani

    2007-07-01

    Full Text Available "nGlucose-6-phosphate dehydrogenase (G6PD deficiency is the most important disease of the hexose monophosphate pathway. Deficiency of this enzym can lead to hemolysis of red blood cells. Our aim was to study the prevalence of G6PD deficiency in relation to neonatal jaundice. We studied 456 clinically icteric neonates Laboratory investigations included determination of direct and indirect serum bilirubin concentrations, blood group typing, direct coomb's test, hemoglobin, blood smear, reticulocyte count and G6PD level. We divided these neonates to 3 groups based on total serum bilirubin level (TSB: TSB< 20 mg%, TSB=20-25 mg%, and TSB>25 mg%. In only 35 (7.6% of cases G6PD deficiency was diagnosed. All of these babies were male. From 456 icteric neonates, 213 cases belong to group 1 (TSB<20 mg%, 158 cases belong to group 2 (TSB=20-25 mg% and 85 cases belong to group 3 (TSB>25 mg%. 16 neonates from 213 neonates of group 1, 6 neonates from 158 neonates of group 2 and 13 neonates from 85 neonates of group 3 had G6PD deficiency. There was statistically significant difference of prevalence of G6PD deficiency between group 2 and 3 ( 15.3% vs 3.8%( P = 0.001. Between groups 1 vs 2 and 1 vs 3 no statistically significant difference was found. Early detection of this enzymopathy regardless of sex and close surveillance of the affected newborns may be important in reducing the risk of severe hyperbilirubinemia. This emphasizes the necessity of neonatal screening on cord blood samples for G6PD deficiency.

  7. Neutron total cross section of sulfur: Single level to multilevel to optical model

    This paper is a further analysis of the high resolution total cross section of sulfur for 25--1100 keV neutrons that previously were measured by Halperin, Johnson, Winters, and Macklin and evaluated by single-level analysis. The usual procedure in reporting the results of high resolution neutron cross sections has been to present the data and resonance parameters with corresponding neutron strength functions resulting from some type of R-matrix analysis. Often the important nonresonant phase shifts are not reported. In this paper, making use of both strength functions and phase shifts, we extend the analysis to include an average nuclear potential (a spherical optical model). An optical model analysis not only facilitates comparison with a broad spectrum of other nucleon-nucleus experiments, but also may provide an incentive for microstructure calculations. Six average empirical functions, two each for s/sub 1/2/, p/sub 1/2/, and p/sub 3/2/ partial waves, are derived from the R-matrix analysis. From these we deduce optical model parameters, the real and imaginary well depths for s- and p-wave neutrons, and the spin-orbit well depth for p waves. The resulting real well is deeper for p waves than for s waves and for averages over partial waves at higher energies. The depth of the imaginary wells are about half those deduced at higher energies. An interesting feature of the analysis is that the multilevel curve including interference effects is produced from single-level parameters including the phase shifts

  8. Measurement of Deformations by MEMS Arrays, Verified at Sub-millimetre Level Using Robotic Total Stations

    Tomas Beran

    2014-06-01

    Full Text Available Measurement of sub-millimetre-level deformations of structures in the presence of ambienttemperature changes can be challenging. This paper describes the measurement of astructure moving due to temperature changes, using two ShapeAccelArray (SAAinstruments, and verified by a geodetic monitoring system. SAA is a geotechnicalinstrument often used for monitoring of displacements in soil. SAA uses micro-electro-mechanical system (MEMS sensors to measure tilt in the gravity field. The geodeticmonitoring system, which uses ALERT software, senses the displacements of targetsrelative to control points, using a robotic total station (RTS. The test setup consists of acentral four-metre free-standing steel tube with other steel tubes welded to most of itslength. The central tube is anchored in a concrete foundation. This composite “pole” isequipped with two SAAs as well as three geodetic prisms mounted on the top, in the middle,and in the foundation. The geodetic system uses multiple control targets mounted inconcrete foundations of nearby buildings, and at the base of the pole. Long-termobservations using two SAAs indicate that the pole is subject to deformations due to cyclicalambient temperature variations causing the pole to move by a few millimetres each day. Ina multiple-day experiment, it was possible to track this movement using SAA as well as theRTS system. This paper presents data comparing the measurements of the two instrumentsand provides a good example of the detection of two-dimensional movements of seeminglyrigid objects due to temperature changes.

  9. Salmonelas e coliformes fecais em águas de bebida para animais Salmonellas and fecal conforms in animal drinking water

    Luiz Carlos de Souza

    1992-10-01

    Full Text Available De um total de 402 propriedades rurais do Município de Botucatu, SP (Brasil com atividades de exploração pecuária, foram sorteadas 60 (15% pelo processo de amostragem probabilística simples. Nestas 60 propriedades localizaram-se 113 bebedouros que eram os mais utilizados pelos animais para sua dessedentação. Foram colhidas amostras de água desses bebedouros visando a pesquisa de bactérias do gênero Salmonella, a determinação do Número Mais Provável (NMP de bactérias coliformes fecais e a verificação do pH e da temperatura. Obteve-se isolamento positivo para salmonelas nas amostras de água de 15 (13,27% bebedouros correspondentes a 12 (20% das 60 propriedades visitadas, identificando-se os seguintes sorotipos: S. dublin, S. newport, S. madelia, S. IV 43:g, z57: -, S. saphra, S. glostrup, S. IV ochsenzool, S. I 9, 12:i: - e dois novos sorotipos S. IV 41:z52: - e S. IV 50:d:-. Das 113 amostras de água analisadas, 14 (12,39% apresentaram-se com NMP/100 mL de coliformes fecais acima de 4.000. Não foi constatada associação entre o isolamento positivo de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000. O isolamento de salmonelas e NMP/100 mL de coliformes fecais acima de 4.000 deu-se em temperaturas superiores a 18°C e em pH entre 6,0 e 7,0.Sixty small farms of Botucatu County, S.Paulo, Brazil, given over to cattle breeding were studied. These farms account for 15% of the total of 402 farms that exist in the county. The sample was drawn by simple probabilistic technique. There were found to be one hundred and thirteen drinking places located on the farms. Samples of water were taken from these drinking places and examined for bacteria of the genus Salmonella, for the determination of Most Probable Number (MNP of fecal coliform bacteria as well as determination of the water's pH. Water temperature was measured before collection. Samples from 15 drinking places (13.3% were positive for Salmonella. The drinking places

  10. Microplate fecal coliform method to monitor stream water pollution.

    Maul, A.; J. C. Block

    1983-01-01

    A study has been carried out on the Moselle River by means of a microtechnique based on the most-probable-number method for fecal coliform enumeration. This microtechnique, in which each serial dilution of a sample is inoculated into all 96 wells of a microplate, was compared with the standard membrane filter method. It showed a marked overestimation of about 14% due, probably, to the lack of absolute specificity of the method. The high precision of the microtechnique (13%, in terms of the co...

  11. Coliforms Everywhere! Using Microbiology to Teach the Scientific Method

    Cindy R. Cisar

    2010-11-01

    Full Text Available The scientific method is a fundamental concept in science. In this exercise the scientific method is taught as a hands-on investigative laboratory experience. Students generate a hypothesis concerning the environmental distribution of coliforms, design and execute an experimental test of that hypothesis, and analyze the resulting data. The exercise is safe and straightforward. It is appropriate for use in undergraduate laboratory courses for science majors and secondary school students and undergraduate non-majors with the appropriate mathematical backgrounds. Students learn both the process by which science progresses, as well as more advanced concepts in microbiology and statistics.

  12. Thyrocalcitonin: its importance on the regulation of the plasmatic levels in total thyroidectomy

    Effect of thyrocalcitonin ou calcium and blood phosphorus is studied. Twelve patients with adenocarcinoma of the thyroid, previously submited to total thyroidectomy and complementary radioiodine theraphy, are studied

  13. Investigating Storm-Induced Total Water Levels on Complex Barred Beaches

    Cohn, N.; Ruggiero, P.; Walstra, D.

    2013-12-01

    Water levels in coastal environments are not static, but rather vary from a range of factors including mean sea level, tides, storm surge, and wave runup. Cumulatively these superimposed factors determine the total water level (TWL), the extent of which has major implications for coastal erosion and inundation during periods of high energy. Storm-induced, super-elevated water levels pose a threat to low lying coastal regions, as clearly demonstrated by recent events such as Hurricanes Sandy and Katrina. For this reason, the ability to accurately predict the TWL is crucial for both emergency managers and coastal planners. While some components of TWL are well understood (e.g., tides) there is still significant uncertainty in predicting runup, a process that can be a major contributor to instantaneous TWLs. Traditionally, empirical relationships derived from observational field data have been used to estimate runup, including wave setup and both incident and infragravity swash (Stockdon et al., 2006). While these formulations have shown skill in predicting the runup extent on natural beaches, these equations consider only the most basic contributing factors - namely the mean foreshore beach slope, the offshore wave height, and offshore wave period. Not included in these empirical estimates is the role of nearshore morphology on TWLs. However, it has long been recognized that nearshore sandbars act as natural barriers to coastal erosion during storm events by dissipating wave energy far from the beach face. Nonetheless, the influence of nearshore morphology on inner surf zone processes, including wave runup, is poorly understood. Recent pioneering studies (eg., Soldini et al., 2013 and Stephens et al., 2011) have explored the role of simple nearshore features (single Gaussian bars) on swash processes. Many locations in the world, however, are characterized by more complex morphologies such as multiple barred systems. Further, in many such places, including Columbia

  14. Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture (Most Probably Number)

    Dehghan fatemeh; Najarian Negin; Kasravi Alii Reza; Falahat Saeed; Zolfaghari Mohammad Reza; Arjomandzadegan Mohammad; Kalantari Salomeh; Ahmari Gholam Reza; Sarmadian Hossein; Sadrnia Maryam; Ahmadi Azam; Shojapoor Mana

    2014-01-01

    Objective:To analyse molecular detection of coliforms and shorten the time of PCR. Methods:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number (MPN) method for 16 artificial and 101 field samples. The molecular method was also conducted on isolated coliforms from positive MPN samples; standard sample for verification of microbial method certificated reference material; isolated strains from certificated reference material and standard bacteria. The PCR and electrophoresis parameters were changed for reducing the operation time. Results:Results of PCR for lacZ and uidA genes were similar in all of standard, operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex PCR. PCR results were confirmed by MPN culture method by sensitivity 86%(95%CI:0.71-0.93). Also the total execution time, with a successful change of factors, was reduced to less than two and a half hour. Conclusions:Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city. It’s recommended to be used at least as an initial screening test, and then the positive samples could be randomly tested by MPN.

  15. Investigation of Coliform Removal from Drinking Water by Electrolysis

    A. Rahmani

    2008-07-01

    Full Text Available Introduction & Objective: The practice of eliminating pathogenic microorganisms in water dates back to ancient times. The most common methods for water disinfection are using chemicals, Ozonation, Ultra Violet ray, Membrane Processes and etc. There has been considerable interest in disinfection of water by using electrochemical methods in recent years. The main purpose of this study is to conduct experimental investigation of water disinfection by using the electrolysis method. Inactivation and killing Coliform in polluted waters was investigated by different voltage, electrodes (Al, St and Cu and distance between electrodes.Materials & Methods: The polluted water was prepared by adding a colony of coliform growth on EMB in raw water. Experiments were done similarly via the same electrolyzes time, electrodes distance and voltage intensity for all types of combinations of electrodes respectively. Results: The experimental results show that the removal efficiency depends on the voltage and electrodes material. From the experiments carried out at 10 V. and at current intensity of 135 mA, it was found that five-minute period was sufficient for disinfecting water using Stainless Steel electrodes.Conclusion: Due to the results, the electrochemical methods can be proposed as a promising cleaning and purifying method for water disinfection.

  16. THE APPLICABLITY TO COMPANY LEVEL OF TOTAL QUALITY MANAGEMENT IN THE TURKISH ARMY

    GENCER, Cevriye; EŞER, Mustafa

    2010-01-01

    ABSTRACTIn this study, the application of quality teams, which is one of the means of total quality management, has been given in the Ordanance Company in Commandos Brigade in Hakkari. The aim of this paper is to search the application of total guality management to the military systems. For this reason, two different quality teams has been constituted in military. The advantages and disadvantages of this application are given from the point of view of Turkish Army. Key Words: Quality, t...

  17. Effect of total lymphoid irradiation on levels of serum autoantibodies in systemic lupus erythematosus and in rheumatoid arthritis

    The effects of total lymphoid irradiation (TLI) on serum levels of autoantibodies, and of antibodies to diphtheria toxoid, tetanus toxoid, and pneumococcal polysaccharide in patients with lupus nephritis were compared with those previously observed in rheumatoid arthritis (RA) patients. Baseline levels of antibodies to diphtheria toxoid and tetanus toxoid decreased significantly after TLI in patients with lupus and RA, but antibody levels to pneumococcal polysaccharide remained unchanged. After TLI, the levels of antinuclear and anti-DNA antibodies were reduced significantly in lupus, but levels of rheumatoid factor, antinuclear, and antigranulocyte antibodies all tended to increase in RA

  18. Effect of total lymphoid irradiation on levels of serum autoantibodies in systemic lupus erythematosus and in rheumatoid arthritis

    Tanay, A.; Schiffman, G.; Strober, S.

    1986-01-01

    The effects of total lymphoid irradiation (TLI) on serum levels of autoantibodies, and of antibodies to diphtheria toxoid, tetanus toxoid, and pneumococcal polysaccharide in patients with lupus nephritis were compared with those previously observed in rheumatoid arthritis (RA) patients. Baseline levels of antibodies to diphtheria toxoid and tetanus toxoid decreased significantly after TLI in patients with lupus and RA, but antibody levels to pneumococcal polysaccharide remained unchanged. After TLI, the levels of antinuclear and anti-DNA antibodies were reduced significantly in lupus, but levels of rheumatoid factor, antinuclear, and antigranulocyte antibodies all tended to increase in RA.

  19. Dietary factors associated with plasma high molecular weight and total adiponectin levels in apparently healthy women

    Yannakoulia, Mary; Yiannakouris, Nikos; Melistas, Labros; Fappa, Evaggelia; Vidra, Nikoletta; Kontogianni, Meropi D; Mantzoros, Christos S

    2008-01-01

    OBJECTIVE: Our aim was to investigate associations between dietary factors and high molecular weight (HMW) as well as total adiponectin in a sample of apparently healthy adult Mediterranean women. DESIGN AND METHODS: Two hundred and twenty women were enrolled in this study. Anthropometric and body c

  20. Anaerobic incubation of membrane filter cultures for improved detection of fecal coliforms from recreational waters.

    Doyle, J D; Tunnicliff, B; Brickler, S K; Kramer, R E; Sinclair, N. A.

    1984-01-01

    Anaerobic incubation of membrane filter cultures significantly enhanced detection of fecal coliforms in surface-water samples from recreational beaches. In contrast to standard aerobic incubation, anaerobic incubation suppressed overgrowth of masking, noncoliform bacteria but did not increase the frequency of fecal coliform recovery.

  1. Effect of dietary poly unsaturated fatty acids on total brain lipid concentration and anxiety levels of electron beam irradiated mice

    The whole brain irradiation causes injury to the nervous system at various levels. Omega-3 poly unsaturated fatty acids are very much essential for the growth and development of nervous system. Dietary supplementation of these nutrients will promote the development of injured neuronal cells. Therefore this study was undertaken to establish the role of Omega-3 poly unsaturated fatty acids on total brain lipid concentration, lipid peroxidation and anxiety levels in the irradiated mice. The effect of Electron Beam Radiation (EBR) on total brain lipid concentration, lipid peroxidation and anxiety level were investigated in male Swiss albino mice. The study groups were subjected to a sub-lethal dose of EBR and also the flax seed extract and fish oil were given orally to the irradiated mice. Irradiated groups show significant elevation in anxiety levels when compared to control group, indicating the acute radiation effects on the central nervous system. But the oral supplementation of dietary PUFA source decrees the anxiety level in the irradiated group. The analysis of lipid peroxidation showed a significant level of changes when compared between control and radiation groups. Dietary PUFA supplementation showed a significant level of decrease in the lipid peroxidation in the irradiated groups. The observation of total lipids in brain shows decrease in concentration in the irradiated groups, the differences in the variables follow the similar patterns as of that the MDA levels. This study suggests that the dietary intake of PUFAs may help in prevention and recovery of the oxidative stress caused by radiation. (author)

  2. Cell-Free Fetal DNA and Cell-Free Total DNA Levels in Spontaneous Abortion with Fetal Chromosomal Aneuploidy

    Ji Hyae Lim; Min Hyoung Kim; You Jung Han; Da Eun Lee; So Yeon Park; Jung Yeol Han; Moon Young Kim; Hyun Mee Ryu

    2013-01-01

    BACKGROUND: Cell-free fetal DNA and cell-free total DNA in maternal circulation have been proposed as potential markers for noninvasive monitoring of the placental condition during the pregnancy. However, the correlation of and change in cell-free fetal DNA and cell-free total DNA in spontaneous abortion (SA) with fetal chromosomal aneuploidy have not yet been reported. Therefore, we investigated cell-free fetal DNA and cell-free total DNA levels in SA women with fetal chromosomal aneuploidy....

  3. [Effects of a low-fat diet including vegetables on total blood cholesterol levels in the aged].

    Pentimone, F; Bertoncini, P; Laghi, G; Del Corso, L; Giuliano, G

    1990-11-30

    High total serum cholesterol and low HDL levels are major risk factors for cardiovascular disease before the age of 65. Currently in older populations their role has not yet been defined. In the elderly a low-cholesterol diet is therefore preferable to pharmacological treatment, in view of the absence of collateral effects. In this study 40 elderly patients with hypercholesterolemia were divided at random into two groups. They were respectively fed their usual diet and a legume-supplemented normal caloric diet for 8 weeks. Total serum cholesterol levels significantly decreased in the latter group from the 2nd week onward. PMID:2150025

  4. Trichromatic π-Pulse for Ultrafast Total Inversion of a Four-Level Ladder System

    Carles Serrat

    2015-11-01

    Full Text Available We present a numerical solution for complete population inversion in a four-level ladder system obtained by using a full π-pulse illumination scheme with resonant ultrashort phase-locked Gaussian laser pulses. We find that a set of pulse areas such as √3π , √2π , and √3π completely inverts the four-level system considering identical effective dipole coupling coefficients. The solution is consistent provided the involved electric fields are not too strong and it is amply accurate also in the case of diverse transition dipole moments. We study the effect of detuning and chirp of the laser pulses on the complete population inversion using the level structure of atomic sodium interacting with ps and fs pulses as an example. Our result opens the door for multiple applications such as efficient ultrashort pulse lasing in the UV or the engineering of quantum states for quantum computing.

  5. Cell-free fetal DNA and cell-free total DNA levels in spontaneous abortion with fetal chromosomal aneuploidy.

    Ji Hyae Lim

    Full Text Available BACKGROUND: Cell-free fetal DNA and cell-free total DNA in maternal circulation have been proposed as potential markers for noninvasive monitoring of the placental condition during the pregnancy. However, the correlation of and change in cell-free fetal DNA and cell-free total DNA in spontaneous abortion (SA with fetal chromosomal aneuploidy have not yet been reported. Therefore, we investigated cell-free fetal DNA and cell-free total DNA levels in SA women with fetal chromosomal aneuploidy. METHODOLOGY/PRINCIPAL FINDINGS: A nested case-control study was conducted with maternal plasma collected from 268 women in their first trimester of pregnancy. Subjects included 41 SA with normal fetal karyotype, 26 SA with fetal chromosomal aneuploidy, and 201 normal controls. The unmethylated PDE9A gene was used to measure the maternal plasma levels of cell-free fetal DNA. The GAPDH gene was used to measure the maternal plasma levels of cell-free total DNA. The diagnostic accuracy was measured using receiver-operating characteristic (ROC curves. Levels of cell-free fetal DNA and cell-free total DNA were significantly higher in both SA women with normal fetal karyotype and SA women with fetal chromosomal aneuploidy in comparison with the normal controls (P<0.001 in both. The correlation between cell-free fetal DNA and cell-free total DNA levels was stronger in the normal controls (r = 0.843, P<0.001 than in SA women with normal karyotype (r = 0.465, P = 0.002 and SA women with fetal chromosomal aneuploidy (r = 0.412, P = 0.037. The area under the ROC curve for cell-free fetal DNA and cell-free total DNA was 0.898 (95% CI, 0.852-0.945 and 0.939 (95% CI, 0.903-0.975, respectively. CONCLUSIONS: Significantly high levels of cell-free fetal DNA and cell-free total DNA were found in SA women with fetal chromosomal aneuploidy. Our findings suggest that cell-free fetal DNA and cell-free total DNA may be useful biomarkers for the prediction of SA

  6. Association between Plasma PFOA and PFOS Levels and Total Cholesterol in a Middle-Aged Danish Population

    Eriksen, Kirsten T.; Raaschou-Nielsen, Ole; McLaughlin, Joseph K.; Lipworth, Loren; Tjønneland, Anne; Overvad, Kim; Sørensen, Mette

    2013-01-01

    Perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) are used in a variety of consumer products and have been detected worldwide in human blood. Recent studies mainly of highly exposed populations have indicated that PFOA and PFOS may affect serum cholesterol levels, but the magnitude of the effect may be inconsistent across exposure levels. The aim of the present cross-sectional study was to investigate the association between plasma PFOA and PFOS and total cholesterol in a general...

  7. Ethnic differences in maternal total cholesterol and triglyceride levels during pregnancy: the contribution of demographics, behavioural factors and clinical characteristics.

    Vrijkotte, Tanja; Schreuder, Ysbrand; Van Eijsden, Manon; Hutten, Barbara; Jansen, Eugene; Twickler, Marcel; Vissers, Maud

    2011-01-01

    Abstract Background/Objectives: Lipid disturbances during pregnancy may lead to early onset of metabolic diseases in the offspring. However, there is little knowledge on ethnic differences in lipid levels during pregnancy. We evaluated ethnic differences in non-fasting total cholesterol (TC) and triglyceride (TG) levels during early gestation and the role of demographics, behavioural factors and clinical characteristics. Subjects/Methods: Non-diabetic pregnant women (N=3025) fr...

  8. The Association Among Lipoprotein-associated Phospholipase A2 Levels, Total Antioxidant Capacity and Arousal in Male Patients with OSA

    Taha T. Bekci, Mehmet Kayrak, Aysel Kiyici, Emin Maden, Hatem Ari, Zeynettin Kaya, Turgut Teke, Hakan Akilli

    2011-01-01

    Full Text Available Background: The mechanisms of the increased cardiac and vascular events in patients with OSA are not well understood. Arousal which is an important component of OSA was associated with increased sympathetic activation and electrocardiographic changes which prone to arrhythmias. We planned to examine the association among arousal, circulating Lp-PLA2 and total antioxidant capacity in male patients with OSA.Methods: Fifty male patients with newly diagnosed OSA were enrolled the study. A full-night polysomnography was performed and arousal index was obtained. Lp-PLA2 concentrations were measured in serum samples with the PLAC Test. Total antioxidant capacity in patients was determined with Antioxidant Assay Kit.Results: Arousal was positively correlated with LP-PLA2 levels (r=0.43, p=0.002 and was negatively correlated with total antioxidant capacity (r= -0.29, p=0.04. Elevated LP-PLA2 levels and decreased total antioxidant activities were found in the highest arousal quartile compared with the lowest and 2nd quartiles (p=0.02, p=0.05, respectively. LP-PLA2 was an independently predictor of arousal index in regression model (β=0.357, p=0.002Conclusions: This study demonstrated a moderate linear relationship between arousal and LP-PLA2 levels. Also, total antioxidant capacities were decreased in the higher arousal index. Based on the study result, the patients with higher arousal index may be prone to vascular events.

  9. Eficiência na remoção de coliformes em águas cinza através da fitorremediação

    Verena S. Baracuhy

    2015-03-01

    Full Text Available O semiárido é caracterizado pelas irregularidades pluviais e o tratamento de águas cinza para reuso na agricultura com tecnologia ecológica é uma forma de conviver com a seca nessas regiões. Nesse contexto, a pesquisa foi realizada no distrito de Ribeira no município de Cabaceiras/PB objetivando-se avaliar a eficiência da remoção dos coliformes totais e fecais do efluente tratado nas unidades de produção agrícola através da fitorremediação. Foram realizados dois tratamentos com e sem fitorremediação, a cultura utilizada foi o capim roxo. As águas cinza eram provenientes de uma lavanderia pública. Foram coletadas amostras mensais e analisadas os coliformes total e fecal (Escherichia coli. No tratamento sem fitorremediação, os coliformes totais variaram de 1,583 x 103 a 2,0224 x 103 (UFC/100 mL e quando o tratamento tinha o auxílio do capim roxo, ou seja, com fitorremediação os coliformes totais oscilaram de 1,354 x 103 a 2,0224 x 103 (UFC/100 mL. Ocorreu uma redução significativa de Escherichia coli no tratamento com fitorremediação. A utilização da fitorremediação no tratamento de águas cinza reduziu a presença de coliformes totais e fecal, sendo assim uma alternativa viável de tratamento, proporcionando um aumento da qualidade hídrica.

  10. Measurement of Deformations by MEMS Arrays, Verified at Sub-millimetre Level Using Robotic Total Stations

    Tomas Beran; Lee Danisch; Adam Chrzanowski; Maciej Bazanowski

    2014-01-01

    Measurement of sub-millimetre-level deformations of structures in the presence of ambienttemperature changes can be challenging. This paper describes the measurement of astructure moving due to temperature changes, using two ShapeAccelArray (SAA)instruments, and verified by a geodetic monitoring system. SAA is a geotechnicalinstrument often used for monitoring of displacements in soil. SAA uses micro-electro-mechanical system (MEMS) sensors to measure tilt in the gravity field. The geodeticmo...

  11. Total serum cholesterol levels and suicide attempts in child and adolescent psychiatric inpatients

    Plana, Teresa; Gracia, R.; Méndez, I.; Pintor, L.; Lazaro, L; Castro-Fornieles, J.

    2010-01-01

    Abstract Associations between cholesterol and suicidal behavior in adolescent patients have not been explored in depth. In this study, 66 patients consecutively admitted to a psychiatric inpatient unit following attempted suicide were compared with a control group of 54 patients with no history of suicide attempts. The age range of the sample was from 8 to 18 years old. Cholesterol levels were significantly lower in attempted suicide patients than in controls (p < 0.02), supporting...

  12. Phenotypic and Phylogenetic Identification of Coliform Bacteria Obtained Using 12 Coliform Methods Approved by the U.S. Environmental Protection Agency.

    Zhang, Ya; Hong, Pei-Ying; LeChevallier, Mark W; Liu, Wen-Tso

    2015-09-01

    The current definition of coliform bacteria is method dependent, and when different culture-based methods are used, discrepancies in results can occur and affect the accuracy of identification of true coliforms. This study used an alternative approach to the identification of true coliforms by combining the phenotypic traits of the coliform isolates and the phylogenetic affiliation of 16S rRNA gene sequences with the use of lacZ and uidA genes. A collection of 1,404 isolates detected by 12 U.S. Environmental Protection Agency-approved coliform-testing methods were characterized based on their phylogenetic affiliations and responses to their original isolation media and lauryl tryptose broth, m-Endo, and MI agar media. Isolates were phylogenetically classified into 32 true-coliform, or targeted Enterobacteriaceae (TE), groups and 14 noncoliform, or nontargeted Enterobacteriaceae (NTE), groups. It was shown statistically that detecting true-positive (TP) events is more challenging than detecting true-negative (TN) events. Furthermore, most false-negative (FN) events were associated with four TE groups (i.e., Serratia group I and the Providencia, Proteus, and Morganella groups) and most false-positive (FP) events with two NTE groups, the Aeromonas and Plesiomonas groups. In Escherichia coli testing, 18 out of 145 E. coli isolates identified by enzymatic methods were validated as FN. The reasons behind the FP and FN reactions could be explained through analysis of the lacZ and uidA genes. Overall, combining the analyses of the 16S rRNA, lacZ, and uidA genes with the growth responses of TE and NTE on culture-based media is an effective way to evaluate the performance of coliform detection methods. PMID:26116679

  13. Total copper, manganese, and zinc levels in a Cecil soil during ten years of poultry litter application

    Heavy metals in poultry litter (PL) can cause environmental problems despite the cost-effectiveness of PL as source of plant nutrients. We compared total Cu, Mn, and Zn levels in a Cecil soil near Watkinsville, GA, in a 5-yr of cotton and 5-yr of corn study under conventional tillage (CT) and no-til...

  14. Differences in urinary prothrombin fragment 1 + 2 levels after total hip replacement in relation to venous thromboembolism and bleeding events

    Borris, L C; Breindahl, M; Lassen, M R;

    2008-01-01

    BACKGROUND: Prothrombin fragment 1 + 2 is excreted in urine (uF1 + 2) as a result of thrombin generation and, therefore, may be a useful marker of coagulation status. OBJECTIVES: To assess uF1 + 2 levels after total hip replacement (THR) in patients with venous thromboembolism (VTE) and bleeding ...

  15. Response of atmospheric ground level temperatures to changes in the total solar irradiance

    Erlykin, Anatoly

    2015-01-01

    The attribution of part of global warming to changes in the total solar irradiance (TSI) is an important topic which is not, yet, fully understood. Here, we examine the TSI induced temperature (T) changes on a variety of time scales, from one day to centuries and beyond, using a variety of assumptions. Also considered is the latitude variation of the T-TSI correlations, where it appears that over most of the globe there is a small increase in the sensitivity of temperature to TSI in time. It is found that the mean global sensitivity (alpha)measured in K(Wm-2)-1 varies from about 0.003 for 1 day, via 0.05 for 11-years to about 0.2 for decades to centuries. We conclude that mean global temperature changes related to TSI are not significant from 1975 onwards. Before 1975, when anthropogenic gases were less important, many of the temperature changes can be attributed to TSI variations. Over much longer periods of time, from Kyear to Myear, the TSI changes are more efficient still, the sensitivity alpha increasing...

  16. Effect of Buddhist meditation on serum cortisol and total protein levels, blood pressure, pulse rate, lung volume and reaction time.

    Sudsuang, R; Chentanez, V; Veluvan, K

    1991-09-01

    Serum cortisol and total protein levels, blood pressure, heart rate, lung volume, and reaction time were studied in 52 males 20-25 years of age practicing Dhammakaya Buddhist meditation, and in 30 males of the same age group not practicing meditation. It was found that after meditation, serum cortisol levels were significantly reduced, serum total protein level significantly increased, and systolic pressure, diastolic pressure and pulse rate significantly reduced. Vital capacity, tidal volume and maximal voluntary ventilation were significantly lower after meditation than before. There were also significant decreases in reaction time after mediation practice. The percentage decrease in reaction time during meditation was 22%, while in subjects untrained in meditation, the percentage decrease was only 7%. Results from these studies indicate that practising Dhammakaya Buddhist meditation produces biochemical and physiological changes and reduces the reaction time. PMID:1801007

  17. Observations of total electron content perturbations on GPS signals caused by a ground level explosion

    Fitzgerald, T. Joseph

    1997-05-01

    We have measured perturbations of electron density in the ionosphere caused by a ground level explosion with an energy release of 2 kt (8.5 × 1012 J) using transmissions from Global Positioning System (GPS) satellites to monitor integrated electron density. The frequencies of the transmissions were 1575.42 MHz (L1) and 1227.60 MHz (L2). The detected perturbation showed a maximum excursion of 0.14 TEC units and had a duration of 80 s beginning at 565 s after the explosion. The acoustic disturbance necessary to produce such a perturbation is well modeled as an N wave with a dimension of 35 km and a relative amplitude of 12% propagating radially at a speed of 0.7 km/s. The majority of the TEC perturbation occurred at an altitude of approximately 200 km.

  18. Pengaruh Ketebalan Media dan Rate filtrasi pada Sand Filter dalam Menurunkan Kekeruhan dan Total Coliform

    Deni Maryani; Ali Masduqi; Atiek Moesriati

    2014-01-01

    Pada penelitian ini dilakukan proses filtrasi dengan menggunakan sand filter sebagai salah satu metode dalam pengolahan air bersih. Pada sand filter proses penyaringan terjadi pada media filter yang sangat halus, seperti media filter pada unit slow sand filter. Kecepatan penyaringan yang diinginkan pada sand filter ini adalah kecepatan seperti pada unit rapid sand filter. Sehingga sand filter ini adalah penggabungan antara kelebihan yang dimiliki slow sand filter dan rapid sand filter. Varias...

  19. 75 FR 14607 - Small Entity Compliance Guide: Bottled Water: Total Coliform and E. coli

    2010-03-26

    ... forth in plain language the requirements of the regulation and to help small businesses understand the... Law 104-121), FDA is making available this SECG stating in plain language the legal requirements of.... Background In the Federal Register of May 29, 2009 (74 FR 25651), FDA issued a final rule amending...

  20. 75 FR 40925 - National Primary Drinking Water Regulations: Revisions to the Total Coliform Rule

    2010-07-14

    ... Technology C Celsius CA Corrective Action CBI Confidential Business Information CCR Consumer Confidence... notification and Consumer Confidence Report language to reflect the construct of the proposed rule. See... Environmental Technology Verification FR Federal Register GW Ground Water GWR Ground Water Rule GWS Ground...

  1. 78 FR 10269 - National Primary Drinking Water Regulations: Revisions to the Total Coliform Rule

    2013-02-13

    ... Technology C--Celsius CCR--Consumer Confidence Report CDC--Centers for Disease Control and Prevention CFR... Consumer Confidence Report language to reflect the construct of the rule. See sections III.F, Violations... publications listed in the rule is approved by the Director of the Federal Register (FR) as of April 15,...

  2. Total and dialyzable levels of manganese from duplicate meals and influence of other nutrients: Estimation of daily dietary intake.

    Velasco-Ryenold, Carlos; Navarro-Alarcón, Miguel; Lopez-Ga De La Serrana, Herminia; Perez-Valero, Vidal; Lopez-Martinez, Maria C

    2008-07-01

    Both total and dialyzable Mn levels were determined in 108 duplicate meals during 36 consecutive days. Both mineral fractions were measured by a graphite furnace atomic absorption spectrometry (GFAAS) method previously optimized. A total mean Mn fraction of 1.03±0.49mg was found in the meals. The Mn supplied by the meals is directly and significantly (pprotein). The mean Mn fraction dialyzed through the dialysis membrane was 0.23±0.17mg (22.0±8.93% as bioaccessible fraction). The total and dialyzable Mn fractions found for breakfasts were significantly lower (ppercentage of dialyzable element, were not significantly different among the three primary meals (breakfast, lunch and dinner). A significant correlation between the total and the dialyzable fraction of Mn in meals was found (pprotein and several amino acid levels (p cereals, legumes, vegetables and fruits, would be primary sources of bioaccessible Mn in the diet. The bioaccessibility of Mn was only significant influenced by energy, carbohydrates and Se levels present in meals. The mean Mn daily dietary intake (DDI) was 3.05±0.61mgday(-1). PMID:26054271

  3. Assessment of serum selenium levels in 2-month-old sucking calves using Total Reflection Technique

    The assessment of selenium status of livestock is an important aspect of production medicine as evidence for the influence of low Se levels on disease resistance in ruminants is reviewed with emphasis on susceptibility to various pathologies (such as infections, exudative diathesis, pneumonia, pancreatic degeneration). Additional evidence suggests that Se deficiency may cause muscular dystrophy in calves, while, severe deficiency has been associated with cardiomyopathy and even death. Serum Se content is a good indicator of the short term Se status and reflects the recent dietary intake of the element. Since Serum Se content is a good indicator of the short term status of the element and reflects the its recent dietary intake, the present work is aimed to determine Se concentration in serum from a group of 2 month old sucking calves suspected to be severely deficient. We used the TX 2000 X-ray spectrometer manufactured by Ital Structures. The energy resolution (FWHM) of the Si(Li) detector was 137 eV for Mn Kα. Among nuclear techniques the TXRF method is the best suitable for trace element analysis in liquid or dissolved samples and can deal much easier with elemental investigation. Physical basis of used analytical method, experimental set up and the sample preparation procedure are described. The concentration data obtained are presented and discussed

  4. Assessment of serum selenium levels in 2-month-old sucking calves using total reflection technique

    The assessment of selenium status of livestock is an important aspect of production medicine as evidence for the influence of low Se levels on disease resistance in ruminants is reviewed with emphasis on susceptibility to various pathologies (such as infections, exudative diathesis, pneumonia, pancreatic degeneration). Additional evidence suggests that Se deficiency may cause muscular dystrophy in calves, while, severe deficiency has been associated with cardiomyopathy and even death. Serum Se content is a good indicator of the short term Se status and reflects the recent dietary intake of the element. Since serum Se content is a good indicator of the short term status of the element and reflects the its recent dietary intake, the present work is aimed to determine Se concentration in serum from a group of 2 months old sucking calves suspected to be severely deficient. We used the TX 2000 X-ray spectrometer manufactured by Ital Structures. The energy resolution (FWHM) of the Si(Li) detector was 137 eV for Mn Kα. Among nuclear techniques the TXRF method is the best suitable for trace element analysis in liquid or dissolved samples and can deal much easier with elemental investigation. Physical basis of used analytical method, experimental set up and the sample preparation procedure are described. The concentration data obtained are presented and discussed. (author)

  5. Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer

    Gastroesophageal reflux is suspected to be an etiological factor in laryngeal and pharyngeal cancer. The aim of this study was to establish, using a non-invasive method, whether laryngopharyngeal reflux (LPR) appears more often in patients with early laryngeal cancer than in a control group. We compared the pH, the level of bile acids, the total pepsin and the pepsin enzymatic activity in saliva in a group of 30 patients with T1 laryngeal carcinoma and a group of 34 healthy volunteers. The groups differed significantly in terms of levels of total pepsin and bile acids in the saliva sample. Higher levels of total pepsin and bile acids were detected in the group of cancer patients. No significant impact of other known factors influencing laryngeal mucosa (e.g. smoking, alcohol consumption, and the presence of irritating substances in the workplace) on the results of saliva analysis was found. A higher level of typical components of LPR in the saliva of patients with early laryngeal cancer than in the controls suggests the possibility that LPR, especially biliary reflux, has a role in the development of laryngeal carcinoma

  6. Removal of N, P, BOD5, and coliform in pilot-scale constructed wetland systems.

    Jin, Guang; Kelley, Tim; Freeman, Mike; Callahan, Mike

    2002-01-01

    Pilot-scale surface-flow (SF), subsurface-flow (SSF), and floating aquatic plant (FAP) constructed wetland system designs were installed and evaluated to determine the effectiveness of constructed wetlands to treat tertiary effluent wastewater in a Midwestern U.S. climate (central Illinois). Average ammonia-nitrogen (N) concentrations decreased approximately 50% in the SSF system design, suggesting that this design had the highest nitrification rate. Nitrate-N concentrations decreased by over 60% in the FAP system design, possibly due to dissimilatory reduction or plant uptake. Total phosphorus (P) concentration reductions of 25 to 40% were observed in all three system designs. Five-day biochemical oxygen demand (BOD5) and dissolved oxygen (DO) results suggested that biodegradation was highest in the SSF system design and lowest in the FAP system design. Greater than 90% concentration reductions of total coliform and E. coli recovered were also observed following treatment in all three system designs. The FAP system design appeared to yield the highest concentration reduction efficiency for E. coli, possibly due to increased sunlight and related bacteriocidal ultraviolet light exposure. Ongoing experiments will test regularly for a variety of vegetative, water quality, and biological conditions for longer time periods in order to gain a better understanding of the pilot constructed wetland system design kinetics. PMID:12655806

  7. EFFECT OF WASTEWATER DISINFECTANTS ON SURVIVAL OF R-FACTOR COLIFORM BACTERIA

    The objectives of the study were to determine the incidence of antibiotic resistance among coliform bacteria in a secondary waste-water treatment facility and to determine whether various alternative disinfection procedures would select for or against antibiotic resistant colifor...

  8. Genome-wide scan on total serum IgE levels identifies FCER1A as novel susceptibility locus.

    Stephan Weidinger

    2008-08-01

    Full Text Available High levels of serum IgE are considered markers of parasite and helminth exposure. In addition, they are associated with allergic disorders, play a key role in anti-tumoral defence, and are crucial mediators of autoimmune diseases. Total IgE is a strongly heritable trait. In a genome-wide association study (GWAS, we tested 353,569 SNPs for association with serum IgE levels in 1,530 individuals from the population-based KORA S3/F3 study. Replication was performed in four independent population-based study samples (total n = 9,769 individuals. Functional variants in the gene encoding the alpha chain of the high affinity receptor for IgE (FCER1A on chromosome 1q23 (rs2251746 and rs2427837 were strongly associated with total IgE levels in all cohorts with P values of 1.85 x 10(-20 and 7.08 x 10(-19 in a combined analysis, and in a post-hoc analysis showed additional associations with allergic sensitization (P = 7.78 x 10(-4 and P = 1.95 x 10(-3. The "top" SNP significantly influenced the cell surface expression of FCER1A on basophils, and genome-wide expression profiles indicated an interesting novel regulatory mechanism of FCER1A expression via GATA-2. Polymorphisms within the RAD50 gene on chromosome 5q31 were consistently associated with IgE levels (P values 6.28 x 10(-7-4.46 x 10(-8 and increased the risk for atopic eczema and asthma. Furthermore, STAT6 was confirmed as susceptibility locus modulating IgE levels. In this first GWAS on total IgE FCER1A was identified and replicated as new susceptibility locus at which common genetic variation influences serum IgE levels. In addition, variants within the RAD50 gene might represent additional factors within cytokine gene cluster on chromosome 5q31, emphasizing the need for further investigations in this intriguing region. Our data furthermore confirm association of STAT6 variation with serum IgE levels.

  9. Total antioxidant status of zinc, manganese, copper and selenium levels in rats exposed to premium motor spirit fumes

    Patrick O Okuonghae

    2011-01-01

    Full Text Available Background: Frequent exposure to premium motor spirit (PMS is common and could be a risk factor for liver dysfunction in those occupationally exposed. A possible association between PMS fumes and plasma total antioxidant status as well as plasma levels of zinc, manganese, copper and selenium using a rodent model could provide new insights into the pathology of the liver where cellular dysfunction is an established risk factor. Aim: This study aimed to determine the total antioxidant status and plasma levels of zinc, copper, selenium and manganese in those occupationally exposed using rodent model. Materials and Methods: 25 albino Wistar rats of both sexes were used for this study. The animals were divided into five groups of five rats in each group. Group 1 rats were not exposed to PMS fumes (control group, group 2 rats were exposed for 1 hour daily, group 3 for 3 hours daily, group 4 for 5 hours daily and group 5 for 7 hours daily. The experiment lasted for a period of 4 weeks. Blood samples obtained from all the groups after 4 weeks of exposure were used for the determination of plasma total antioxidant status as well as plasma levels of zinc, manganese, copper and selenium. Results: Results showed significant increases in means of plasma copper (69.70±0.99 for test and 69.20±1.02 for control, P < 0.05 and selenium (72.70±1.58 for test and 68.20±0.86 for control, P < 0.05 in the exposed rats when respective mean values were compared with those of corresponding controls. Mean body weight index (BWI and percentage weight increase (PWI were significantly lower (P < 0.05 in exposed rats when compared with the unexposed group. The mean plasma levels of zinc (137.40±4.06 for test and 147.80±2.52 for control and manganese (65.75±1.02 for test and 70.00±0.71 for control showed significant decrease (P < 0.05 when compared with control. Plasma level of total antioxidant status (TAS did not differ significantly in exposed rats when compared with

  10. Urinary IgG antibody against mixed heat-killed coliform antigen and lipopolysaccharide core antigen.

    Gibb, A P; Edmond, D. M.

    1992-01-01

    AIMS: To determine whether antibody to lipopolysaccharide-core (LPS-core) antigen is an important component of the antibody, detected by mixed heat-killed coliform antigen, in urine from patients with suspected urinary tract infection. METHODS: LPS-core antigen and mixed heat-killed coliform antigen were used in an enzyme linked immunosorbent assay (ELISA) to measure IgG antibody in midstream urine samples. Seventy two samples from students attending their general practitioner with symptoms s...

  11. Antibiotic Resistance of Enterococci and Coliform Bacteria in Dairy Products from Commercial Farms

    Ivana Nováková; Miroslava Kačániová; Henrieta Arpášová; Peter Haščík; Simona Kunová; Juraj Čuboň

    2010-01-01

    The aim of this study was to determine the prevalence and antibiotic resistance of enterococci and coliform bacteria isolated from sheep and cows cheese from commercial farms. Susceptibilities of isolated enterococci and coliform bacteria were tested using the disk diffusion method. The bacteria were tested on antibiotics enrofloxacin, sulphonamides, tetracycline and streptomycin. All isolates of Enterococcus strains were resistant of all used antibiotics. The similar results were detected of...

  12. The effects of oral and intramuscular administration and dose escalation of enrofloxacin on the selection of quinolone resistance among Salmonella and coliforms in pigs

    Wiuff, C.; Lykkesfeldt, J.; Svendsen, O.;

    2003-01-01

    The effect of route of administration and dose of enrofloxacin (Baytril(R)) on the development of fluoroquinolone resistance in Salmonella and Escherichia coli in the intestinal tract of pigs was investigated. Healthy pigs at the age of 8-10 weeks were infected with a mixture of susceptible wild......-type (MICciprofloxacin = 0.03 mug/ml) and a mutant Salmonella typhimurium with reduced susceptibility to fluoroquinolones (MICciprofloxacin 0.5 mug/ml) (in the ratio 99: 1) and treated with 2.5 mg/kg bwt enrofloxacin by either intramuscular (i.m.) or oral (p.o.) administration at time points either 4 or 24 It after the...... resistant and total number of coliforms and Salmonella in faeces of the pigs. High frequencies of fluoroquinolone resistance developed rapidly among the coliform flora independent of route of administration, dose or time of initiation of the treatment. Selection for resistance among the artificially...

  13. Application of annular centrifugal contactors in the hot test of the improved total partitioning process for high level liquid waste

    Highlights: • An improved total partitioning process for high level liquid waste was developed. • Genuine high level liquid waste was used in the hot test. • 72-Stage 10-mm-dia annular centrifugal contactors were used in the hot test. • The decontamination factors of actinides, Sr and Cs were very high. • The stripping efficiencies of actinides, Sr and Cs were very high. - Abstract: High level liquid waste (HLLW) produced from the reprocessing of the spent nuclear fuel still contains moderate amounts of uranium, transuranium (TRU) actinides, 90Sr, 137Cs, etc., and thus constitutes a permanent hazard to the environment. The partitioning and transmutation (P and T) strategy has increasingly attracted interest for the safe treatment and disposal of HLLW, in which the partitioning of HLLW is one of the critical technical issues. An improved total partitioning process, including a TRPO (tri-alkylphosphine oxide) process for the removal of actinides, a CESE (crown ether strontium extraction) process for the removal of Sr, and a CECE (calixcrown ether cesium extraction) process for the removal of Cs, has been developed to treat Chinese HLLW. A 160-hour hot test of the improved total partitioning process was carried out using 72-stage 10-mm-dia annular centrifugal contactors (ACCs) and genuine HLLW. The hot test results showed that the average DFs of total α activity, Sr and Cs were 3.57 × 103, 2.25 × 104 and 1.68 × 104 after the hot test reached equilibrium, respectively. During the hot test, 72-stage 10-mm-dia ACCs worked stable, continuously with no stage failing or interruption of the operation

  14. Identifikasi Bakteri Coliform pada Air Kobokan di Rumah Makan Kelurahan Andalas Kecamatan Padang Timur

    Ervan Arditya Kusuma

    2015-09-01

    Full Text Available Abstrak Air kobokan merupakan media untuk cuci tangan yang banyak digunakan di rumah makan di  Kota Padang. Diare adalah penyakit yang ditularkan melalui air yang terkontaminasi oleh bakteri. Angka perkiraan kasus diare di Padang Timur merupakan yang tertinggi di Kota Padang. Tujuan penelitian ini adalah untuk mengetahui kualitas mikrobiologi air kobokan di rumah makan di Kelurahan Andalas Kecamatan Padang Timur. Sebanyak 21 sampel yang diambil dan dibagi sesuai dengan sumber airnya yaitu 12 sumber air PDAM dan 9 sumber air sumur bor. Penelitian ini dibagi dalam dua tahap, tahap pertama:  pengambilan sampel dan observasi faktor yang mempengaruhi kontaminasi. Kedua: uji bakteriologi dengan metode Most Propable Number (MPN yang meliputi uji penduga, uji konfirmasi dan uji lengkap.Seluruh sampel air kobokan mengandung bakteri coliform. Sebagian besar sampel tidak memenuhi syaratmikrobiologis sesuai dengan PERMENKES no 46 tahun 1990 dan hanya satu sampel yang berasal dari air PDAM memenuhi syarat mikrobiologis. Sampel yang berasal dari PDAM didapatkan 4 yang mengandung E.coli dan dari sumber sumur bor didapatkan 3 sampel mengandung E.coli dan lainnya mengandung bakteri lain seperti Klebsiella, Enterobacter dan Pseudomonas. Hal ini dipengaruhi oleh banyak faktor seperti kebersihan wadah, lokasi rumah makan dan lokasi sumur bor.Kata kunci: air kobokan, most propable number (MPN. Abstract Water is the common use for hand washing in restaurant in Padang city. The research was conducted at restaurant in Andalas, East Padang. Diarrhea is a disease transmitted through water contaminated by bacteria.Morbidity of diarrhea in East Padang is the highest in Padang. The objective of this study was to identify microbiological quality of water for cleaning hand at Restaurant in Andalas sub-district, East Padang district. Total 21 sampels were taken and divided according to the water source is 12 by PDAM and 9 from dug well. This research implemented in two

  15. A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage

    Willy Jablonka

    2011-08-01

    Full Text Available Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP activity. Similarly, phosphotyrosine (pTyr-containing bands are seen in extracts prepared from both male and female heads and are spread among a variety of structures including the antennae, proboscis and the maxillary palps combined with the proboscis. Also, mosquitoes treated with sodium orthovanadate, a classical PTP inhibitor, show reduced blood-feeding activity and higher head tyrosine phosphorylation levels. These results suggest that pTyr-mediated signalling pathways may play a role in the initial days following the emergence of the adult mosquito from the pupal stage.

  16. Impacts of sanitation upgrading to the decrease of fecal coliforms entering into the environment in China.

    Tong, Yindong; Yao, Ruihua; He, Wei; Zhou, Feng; Chen, Cen; Liu, Xianhua; Lu, Yiren; Zhang, Wei; Wang, Xuejun; Lin, Yan; Zhou, Min

    2016-08-01

    Identifying the sanitation efficacy of reducing fecal contaminations in the environment is important for evaluating health risks of the public and developing future management strategies to improve sanitation conditions. In this study, we estimated the fecal coliforms (FC) entering into the environment in 31 provinces in China under three sanitation scenarios. Our calculation results indicated that, the current FC release is disparate among regions, and the human releases in the rural regions were dominant, accounting for over 90% of the total human releases. Compared with the human release, the FC release from the livestock was of similar magnitude, but has a quite different spatial distribution. In China Women's Development Program, the Chinese government set the target to make over 85% of the population in the rural access to the toilets in 2020. If the target set by the Chinese government is achieved, a decrease of 34% (12-54%) in the FC releases would be anticipated. In the future, the improvement in sanitation and accesses to the safe drinking water in the less developed regions, such as Tibet, Qinghai, and Ningxia, should be considered as a priority. PMID:27179203

  17. Monitoring of human enteric viruses and coliform bacteria in waters after urban flood in Jakarta, Indonesia.

    Phanuwan, C; Takizawa, S; Oguma, K; Katayama, H; Yunika, A; Ohgaki, S

    2006-01-01

    Floodwaters in Kampung Melayu village, Jakarta, Indonesia, as well as river water and consumable water (including groundwater and tap water) samples in flooded and non-flooded areas, were quantitatively analysed to assess occurrence of viruses and total coliforms and E. coli as bacterial indicators after flooding event. High numbers of enterovirus, hepatitis A virus, norovirus (G1, G2) and adenovirus were detected at high concentration in floodwaters and waters sampled from Ciliwung River which runs across metropolitan Jakarta and is used widely for agriculture and domestic purposes by poor residents. One out of three groundwater wells in the flooded area was contaminated with all viruses tested while no viruses were found in groundwater samples in non-flooded areas and tap water samples. The results revealed that human enteric viruses, especially hepatitis A virus and adenovirus, were prevalent in Jakarta, Indonesia. This study suggested that flooding posed a higher risk of viral infection to the people through contamination of drinking water sources or direct contact with floodwaters. PMID:17037154

  18. Association between apolipoprotein E promoter-219G/T polymorphism and total cholesterol level in patients with Alzheimer disease

    Fang Liu; Xiao Sun; Jing Wang; Yan Kong; Li Cui; Xiangdang Shi

    2006-01-01

    BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding region, so its polymorphism may result in the abnormality of APOE gene and protein expression,and finally lead to dementia.OBJECTIVE: To observe the association between APOE promoter-219G/T polymorphisms with serum total cholesterol in patients with Alzheimer disease, and compare it with non-dementia people.DESIGN: Case-control, comparative observation.SETTING: Department of Neurology, Fengtian Hospital of Shenyang Medical College.PARTICIPANTS: Fifty-five dementia patients including 27 males and 28 females aged (66±3) years and treated in the Department of Neurology, Fengtian Hospital were selected from January 2002 to December 2005 as the Alzheimer disease group. They all diagnosed according to the DSM- Ⅳ diagnostic criteria of Alzheimer disease instituted by American Psychiatry Association in 1994. Meanwhile, 44 none-dementia patients including 21 males and 23 females aged (66±3) years were selected from other clinical departments of Fengtian Hospital as control group. All the participants were informed the detection and agreed.METHODS: Genomic DNA was extracted from the peripheral blood of all subjects, then "NEST"PCR, DNA sequence and enzyme digestion were adopted to detect the expression of APOE promoter-219 polymorphism,following by biomedical statistics analysis based on the clinical total cholesterol level.MAIN OUTCOME MEASURES: Polymorphism of APOEpromoter-219 G/T and total cholesterol level.RESULTS: All 55 dementia patients and 44 non-dementia ones were involved in the result analysis. ①Allele and genotype frequency: The T allele frequency of the Alzheimer disease group was significantly higher than that in the control group [88.2% (97/110), 54.5% (48/88)], while G allele frequency was remarkably lower than that in the control group [11

  19. Does Co-Existing Lumbar Spinal Canal Stenosis Impair Functional Outcomes and Activity Levels after Primary Total Hip Arthroplasty?

    Jauregui, Julio J; Banerjee, Samik; Issa, Kimona; Cherian, Jeffrey J; Mont, Michael A

    2015-09-01

    Degenerative lumbar spinal stenosis (LSS) is a cause for substantial morbidity in the elderly population: many often undergo total hip arthroplasty for associated hip arthritis. With a matched cohort we investigated the effect of co-existing LSS on aseptic survivorship, functional outcomes, activity levels, overall subjective physical and mental health status, and satisfaction rates in patients undergoing primary THA. The aseptic-implant survivorship was similar in LSS and non-stenosis cohort. Although both cohorts significantly improved, the LSS cohort achieved lower improvements in HHS, UCLA, SF-36 physical, and satisfaction rates than the matched non-stenotic cohort. Surgeons should consider cautioning patients with LSS that although they can expect relief of their arthritic symptoms following THA, they may continue to expect limitations in function, physical-status, activity-levels, and satisfaction rates. PMID:25865814

  20. Serum total homocysteine and lipoprotein (a) levels in acute myocardial infarction and their response to treatment with vitamins serum total homocysteine and lipoprotein (a) levels in acute myocardial infarction and their response to treatment with vitamins

    To assess the relationship of serum total homocysteine (tHcy) and lipoprotein (a) [Lp(a)] levels with systemic hypertension, Diabetes mellitus and smoking as risk factors in patients with acute myocardial infarction (AMI) and changes in the former levels with vitamins supplementation. Study Design: An interventional study. Place and Duration of Study: Medical College for Women and Hospital (MCW and H), Dhaka, Bangladesh, from July 2008 to December 2009. Methodology: Consecutive AMI patients were recruited from the Coronary Care Unit (CCU) at MCW and H, Dhaka. Blood samples were collected at inclusion (Patient-I0). They were given conventional treatments and prescribed vitamins (vitamins B6=25 mg, B12=2 mg and folic acid=2.5 mg) daily for 2 months. After follow-up, blood samples were taken again (Patient-II0). A group of 25 normal subjects were also included as controls. Serum tHcy and Lp(a) were measured by kinetic method and nephelometric method respectively. Results: Serum tHcy (macor mol/L) and Lp(a) (mg/dl) levels were elevated in Patient-I that reduced in Patient-II after vitamins supplementation, but not to the normal control level. tHcy of Patient-I0 was 25.1 +- 4.7 macro mol/L, of Patient-II0 was 20.1 +- 4.5 mu mol/L and of controls 12.1 +- 3.3, p 0.1). However, in a significant proportion of patients tHcy and Lp(a) levels were reduced to control levels (tHcy: p < 0.001, Lp(a): p < 0.01). Conclusion: These results indicated that tHcy and Lp(a) levels were possibly atherogenic risk factors independent of conventional risk factors. Since both tHcy and Lp(a) levels responded in a similar fashion, a common point of the metabolic and pathogenetic pathways of tHcy and Lp(a) may be influenced by the vitamins supplementation. (author)

  1. Evaluación de tres métodos para la inactivación de coliformes y Escherichia coli presentes en agua residual doméstica, empleada para riego

    Andrea Sánchez- Garibello

    2010-08-01

    Full Text Available Evaluation of three methods for the inactivation of coliforms and Escherichia coli present in domestic wastewaters used inirrigation. Objective. To evaluate three treatments (facultative stabilization ponds, heterogeneous photocatalysis with TiO2 and chemicaldisinfection with sodium hypochlorite for the inactivation of coliforms and Escherichia coli present in domestic wastewaters used inagricultural irrigation. Materials and methods. Wastewater was characterized by physical, chemical and microbiological analyses and wasthen exposed to a facultative pond treatment (FPT, post-photocatalytic treatment (PTFTiO2/UV and post-chemical treatment (PTQNaClOto assess the disinfecting capacity of each method in the inactivation of total coliforms and E. coli. Three new samples of wastewater wereprocessed and used in irrigation tests on a laboratory-scale basis for 30 days, using Lactuca sativa cultivar. Batavia as a model plant andevaluating the initial and final concentrations of the two groups. Results. PTFTiO2/UV was significantly higher than FPT and PTQNaClO(p<0.0001, obtaining 100% of inactivation of coliforms and E. coli after 30 minutes of irradiation at a reactor scale. Regarding the irrigationtests with L. sativa, we showed that using water treated by PTFTiO2/UV there is no contamination with E. coli and coliforms after 30 days.On the contrary, plants irrigated with water treated by FPT and PTQNaClO showed an increase in the two populations originating a contamination problem in the vegetable by the end of the laboratory experiments. Conclusion. The heterogeneous photocatalysis with TiO2was an effective method in the reduction of coliforms and E. coli present in domestic wastewater.

  2. Extreme total solar irradiance due to cloud enhancement at sea level of the NE Atlantic coast of Brazil

    Piacentini, Ruben D. [Instituto de Fisica Rosario (CONICET-Universidad Nacional de Rosario), 27 de Febrero 210bis, 2000 Rosario (Argentina); Facultad de Ciencias Exactas, Ingenieria y Agrimensura, Universidad Nacional de Rosario, Pellegrini 250, 2000 Rosario (Argentina); Salum, Graciela M. [Instituto de Fisica Rosario (CONICET-Universidad Nacional de Rosario), 27 de Febrero 210bis, 2000 Rosario (Argentina); Facultad Regional Concepcion del Uruguay, Universidad Tecnologica Nacional, Concepcion del Uruguay (Argentina); Fraidenraich, Naum; Tiba, Chigueru [Grupo de Pesquisas em Fontes Alternativas de Energia, Universidade Federal de Pernambuco, Av. Prof. Luiz Freire, 1000 - 50.740-540, Recife, PE (Brazil)

    2011-01-15

    Extraterrestrial total solar irradiance, usually called Solar Constant, is attenuated by the atmosphere in different proportions, depending mainly on solar zenith angle and altitude of the measurement point. In this work, it is presented very high and extreme horizontal plane measurements of global solar irradiance that in some days overpassed the Solar Constant corrected by the actual Sun-Earth distance (CSC). They were obtained at sea level of the intertropical Atlantic coast, in the city of Recife, Brazil, in the period February 2008-January 2009. Extreme total solar irradiance values larger than CSC were measured during 3.4% of the days of the total registered period. This percentage increases to 7.4% for global solar irradiance within 95.1-100% of the CSC and to 15.3% within 90.1-95% of the CSC. The largest extreme total solar irradiance value, 1477 {+-} 30 W/m{sup 2}, was registered the 28th of March 2008 at 11:34 local time (UT - 3h). It overpassed by 7.9% the CSC value for this day (1369.4 W/m{sup 2}) and by 42.3% the estimated value of the clear sky Iqbal C radiation model (1037.7 W/m{sup 2}). The observation of extreme values should be taken into account in the study of solar radiation effects related to materials exposed to the outside, UV index and biological effects, among others. Also, the detailed knowledge of this interesting effect may contribute significantly to clarify physical aspects about the interaction of global solar radiation with the ecosystem and climate change. (author)

  3. Total Intravenous Versus Inhalation Anesthesia in Patients Undergoing Laparoscopic Cholecystectomies. Effects on Two Proinflammatory Cytokines Serum Levels: Il-32 and TNF-Alfa.

    Hadade Adina

    2016-01-01

    Full Text Available Introduction: It has been reported that as compared with total intravenous anesthesia (TIVA, inhalation anesthesia is increasing the postoperative level of proinflammatory interleukins.

  4. Lake level change and total water discharge in East Africa Rift Valley from satellite-based observations

    Hassan, Ayman A.; Jin, Shuanggen

    2014-06-01

    The measurement of total basin water discharge is important for understanding the hydrological and climatologic issues related to the water and energy cycles. Climatic extreme events are normal climatic occurrences in Africa. For example, extensive droughts are regular features in the last few decades in parts of East Africa, which suffers from a lack of in situ observations as well as a lack of regional hydrological models. In this study, multi-disciplinary different types of space-borne observations and global hydrological models are used to study total water discharge in the Great Rift Valley of East Africa (i.e. Lakes Victoria, Tanganyika, and Malawi) from January 2003 to December 2012. The data include the following: (1) total water storage (TWS) variations from Gravity Recovery and Climate Experiment (GRACE), (2) the lake level variations from Satellite Alimetric data, (3) rainfall from Tropical Rainfall Measurement Mission (TRMM) products, (4) soil moisture from WaterGAP Global Hydrology Model (WGHM), and (5) water fluxes from Global Land Data Assimilation System (GLDAS). Results show that a significant decline in the average lake level is found for all of the three lakes between 2003 and 2006. GRACE TWS variations of the whole basin area show the same pattern of variation as the average lake level variations estimated from Altimetric data. The TWS in the basin area of Lakes Victoria and Malawi is governed by the surface water stored in each lake itself, while for Lake Tanganyika, it is governed by both surface water and the soil moisture content in the basin area. Furthermore, the effect of rainfall on TWS is also studied. A phase lag of ~ 2 months is found between TRMM rainfall and GRACE TWS (generally, rainfall precedes the GRACE TWS) for the three lakes. In addition, the regional evapotranspiration ET is estimated from the water balance equation using GRACE land-water solutions, rainfall data from TRMM and runoff values obtained as a fraction of rainfall

  5. Assessment of serum selenium levels in 2-month-old sucking calves using total reflection X-ray fluorescence technique

    Buoso, M. C.; Ceccato, D.; Moschini, G.; Bernardini, D.; Testoni, S.; Torboli, A.; Valdes, M.

    2001-11-01

    The assessment of selenium status of livestock plays an important role in the production of medicine since low serum Se levels influence disease resistance in ruminants. It has been proved that Se deficiency may cause muscular dystrophy, cardiomyopathy and even death. Serum level has been widely used to evaluate the Se short-term status in animals since there is a good association between serum Se level and the dietary intake of the element over a wide range. The purpose of this work was to determine the Se serum concentration in a population of 78 sucking 2-month-old calves, in order to corroborate a clinical diagnosis of severe deficiency status. The samples were analyzed by total reflection X-ray fluorescence (TXRF) at the ITAL STRUCTURES Research Laboratory. The results obtained from the serum samples presented Se concentrations varying from 10 to 66 ng/ml. The comparison between the obtained values and the expected serum selenium values (60-80 ng/ml), confirmed a mild to severe deficiency status in the investigated population.

  6. Power-Performance Trade-Offs in Nanometer-Scale Multi-Level Caches Considering Total Leakage

    Bai, Robert; Kgil, Tae Ho; Sylvester, Dennis; Mudge, Trevor

    2011-01-01

    In this paper, we investigate the impact of T_{ox} and Vth on power performance trade-offs for on-chip caches. We start by examining the optimization of the various components of a single level cache and then extend this to two level cache systems. In addition to leakage, our studies also account for the dynamic power expanded as a result of cache misses. Our results show that one can often reduce overall power by increasing the size of the L2 cache if we only allow one pair of Vth/T_{ox} in L2. However, if we allow the memory cells and the peripherals to have their own Vth's and T_{ox}'s, we show that a two-level cache system with smaller L2's will yield less total leakage. We further show that two Vth's and two T_{ox}'s are sufficient to get close to an optimal solution, and that Vth is generally a better design knob than T_{ox} for leakage optimization, thus it is better to restrict the number of T_{ox}'s rather than Vth's if cost is a concern.

  7. Adenovirus, enterovirus and thermotolerant coliforms in recreational waters from Lake Guaíba beaches, Porto Alegre, Brazil.

    Maurer, C P; Simonetti, A B; Staggemeier, R; Rigotto, C; Heinzelmann, L S; Spilki, F R

    2015-12-01

    In the present study, molecular detection of human adenoviruses (HAdV) and enteroviruses (EV) was performed in surface water samples collected from beaches Ipanema and Lami, located on the shores of Lake Guaíba, city of Porto Alegre, RS, southern Brazil. Furthermore, water safety was evaluated by counting thermotolerant coliforms (TC), following local government regulations. A total of 36 samples were collected monthly from six different sites along the beaches. Viral genomes were found in 30 (83.3%) samples. The higher detection rate was observed for HAdV (77.8%), followed by EV (22.2%). Although low concentrations of TC have been found, the occurrence of viral genomes in water samples was frequent and may pose a potential risk of infection for people bathing in these beaches. PMID:26608773

  8. Plasma total homocysteine (THCY) level and other biochemical risk factors in hypertensives with and without cardiovascular events

    Akande AA; Salisu OT; Omotoso ABO; Kolo PM

    2009-01-01

    Objective:The aim of this study is to assess the correlation between traditional cardiovascular risk factors and elevated plasma tHcy level in Nigerian hypertensive. Methods: Thirty-six hypertensive patients were recruited with 36 age and sex-matched controls. The age, sex and anthropometric measurements including height, weight and body mass index (BMI) were taken. Plasma total cholesterol, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), tri-glycerides and tHey were analyzed. The results of the two groups were compared. Results: The mean total cholesterol and LDL-C were significantly higher in the subjects than in the controls (P=0.01 and 0.03 respectively). On the other hand, means of HDL-C and triglycerides were not significantly different between the two groups (P = 0.06 and O. 68 respectively). Mean total plasma tHey was (12. 95±4.9)μmol/L in hypertensive patients when compared with (11.29±3.6)μmol/L in the controls (P =0.09), however the mean they was significantly higher in hypertensive patients who had stroke or myocardial infarction than those without these complications (one way Anova F = 3.63, P =0.04). Significant positive correlation was seen between tHcy and systolic blood pressure, diastolic blood pressure, BMI and LDL-C. Conclusion:The study suggests that elevated plasma tH-ey may predict occurrence of cardiovascular complications in hypertensive individuals.

  9. A Prospective, Longitudinal Study of Patient Activity Levels Following Total Knee Arthroplasty Stratified by Demographic and Comorbid Factors.

    Issa, Kimona; Jauregui, Julio J; Given, Kristin; Harwin, Steven F; Mont, Michael A

    2015-08-01

    With the marked increase in the annual number of total knee arthroplasties (TKAs) in the United States, there has been an increased interest in evaluating patient-reported outcomes. The purpose of this study was to prospectively and longitudinally evaluate temporal trends in patient activity levels following TKA and to identify potential demographic and comorbid factors that may affect these outcomes. This prospective study evaluated 281 patients, including 108 men and 173 women, who had a mean age of 66 years (39-80 years) and underwent primary TKA. All patients were followed for a minimum of 2 years. Medical comorbidities were recorded preoperatively and activity scores were evaluated at each follow-up visit. The effects of different patient demographics and systemic comorbidities on activity outcomes were further analyzed using multivariate regression analysis. Compared with preoperative levels, the activity score was observed to initially significantly decrease at 6 weeks postoperatively to below preoperative levels (9.2 vs. 8.1 points). By 3 months, scores were above preoperative levels (10.3 points), but below peak levels. A significant peak in the activity score was observed at 2-year follow-up after which there were no significant differences in scores at 5 years (11.49 vs. 11.47 points). In evaluating patient demographics and comorbidities, significant negative impact of older age, tobacco use, history of cancer, cardiovascular disease, lymphatic disease, and renal disease can be seen on activity levels. Activity scores following TKA follow a temporal trend in which scores initially drop below preoperative levels after surgery, but recover and reach a peak at 2 years postoperatively. This peak was maintained at 5-year follow-up. Surgeons should counsel their patients that they will not perceive the full benefit of a TKA until 1 to 2 years after surgery, in addition, patients may actually perceive their progress to decrease initially. Also, their

  10. Evaluation of Flow Rate, pH, Buffering Capacity, Calcium, Total Protein and Total Antioxidant Levels of Saliva in Caries Free and Caries Active Children—An In Vivo Study

    Prabhakar, AR; Dodawad, Reshma; OS, Raju

    2009-01-01

    Background and objectives: The purpose of this study was to evaluate the relationship between the physicochemical properties of saliva such as flow rate, pH, buffering capacity, calcium level, total protein and total antioxidant levels in caries free and caries active children. Materials and methods: The present study included one hundred and twenty healthy children who were divided into two groups and subdivided according to gender. They were further divided into caries free and caries activ...

  11. Evaluation of total creatine kinase levels in a spectrum of neuro-psychiatric disorders in a tertiary neurosciences centre

    Anshu Gupta

    2015-01-01

    Full Text Available Introduction: To study usefulness of total creatine kinase (CK as a screening tool in various neurological and psychiatric disorders in emergency setting of a tertiary care hospital. Materials and Methods: A 1-year retrospective study was conducted on 102 patients with complaints pertaining to neurological and psychiatric disorders in a tertiary neurosciences centre in a metropolitan city. Blood samples in plain vial were received in Emergency Laboratory and total CK levels were measured by automated analyzer and its correlation with various diseases was analyzed. Results: It was observed that CK activity was raised in various psychiatric conditions-acute transient psychotic disorder, alcohol dependence syndrome, delirium, psychosis, mental retardation, catatonia, bipolar affective disorder (BAD, depression and mania and also in neurological disorders-seizures, meningitis, myasthenia gravis (multiple sclerosis, Guillain-Barre syndrome, extra pyramidal syndrome, neuroleptic malignant syndrome and infarct. Conclusion: This study demonstrated that CK is a sensitive and an important screening parameter in diagnosis and monitoring of various neurological and psychiatric disorders in emergency setting. It is also helpful in identifying people at high risk for various neuro-psychiatric diseases.

  12. How much locomotive activity is needed for an active physical activity level: analysis of total step counts

    Ohkawara Kazunori

    2011-11-01

    Full Text Available Abstract Background Although physical activity recommendations for public health have focused on locomotive activity such as walking and running, it is uncertain how much these activities contribute to overall physical activity level (PAL. The purpose of the present study was to determine the contribution of locomotive activity to PAL using total step counts measured in a calorimeter study. Methods PAL, calculated as total energy expenditure divided by basal metabolic rate, was evaluated in 11 adult men using three different conditions for 24-hour human calorimeter measurements: a low-activity day (L-day targeted at a low active level of PAL (1.45, and a high-frequency moderate activity day (M-day or a high-frequency vigorous activity day (V-day targeted at an active level of PAL (1.75. These subjects were permitted only light activities except prescribed activities. In a separate group of 41 adults, free-living PAL was evaluated using doubly-labeled water (DLW. In both experiments, step counts per day were also measured using an accelerometer. Results In the human calorimeter study, PAL and step counts were 1.42 ± 0.10 and 8,973 ± 543 steps/d (L-day, 1.82 ± 0.14 and 29,588 ± 1,126 steps/d (M-day, and 1.74 ± 0.15 and 23,755 ± 1,038 steps/d (V-day, respectively. In the DLW study, PAL and step counts were 1.73 ± 0.15 and 10,022 ± 2,605 steps/d, and there was no significant relationship between PAL and daily step counts. Conclusions These results indicate that an enormous number of steps are needed for an active level of PAL if individuals extend physical activity-induced energy expenditure by only locomotive activity. Therefore, non-locomotive activity such as household activity should also play a significant role in increasing PAL under free-living conditions.

  13. Single dose testosterone increases total cholesterol levels and induces the expression of HMG CoA Reductase

    Gårevik Nina

    2012-03-01

    Full Text Available Abstract Background Cholesterol is mainly synthesised in liver and the rate-limiting step is the reduction of 3-hydroxy-3methylglutaryl coenzyme A (HMG-CoA to mevalonate, a reaction catalysed by HMG-CoA reductase (HMGCR. There is a comprehensive body of evidence documenting that anabolic-androgenic steroids are associated with deleterious alterations of lipid profile. In this study we investigated whether a single dose of testosterone enanthate affects the cholesterol biosynthesis and the expression of HMGCR. Methods 39 healthy male volunteers were given 500 mg testosterone enanthate as single intramuscular dose of Testoviron®--Depot. The total cholesterol levels prior to and two days after testosterone administration were analysed. Protein expression of HMGCR in whole blood was investigated by Western blotting. In order to study whether testosterone regulates the mRNA expression of HMGCR, in vitro studies were performed in a human liver cell-line (HepG2. Results The total cholesterol level was significantly increased 15% two days after the testosterone injection (p = 0.007. This is the first time a perturbation in the lipoprotein profile is observed after only a single dose of testosterone. Moreover, the HMGCR mRNA and protein expression was induced by testosterone in vitro and in vivo, respectively. Conclusion Here we provide a molecular explanation how anabolic androgenic steroids may impact on the cholesterol homeostasis, i.e. via an increase of the HMGCR expression. Increasing knowledge and understanding of AAS induced side-effects is important in order to find measures for treatment and care of these abusers.

  14. Detection of the coliform bacteria Escherichia coli and Salmonella sp. in water by a sensitive and rapid immunomagnetic electrochemiluminescence (ECL) technique

    Yu, H.; Bruno, J.

    1995-10-01

    Hemorrhagic Escherichia coli O157:H7 and other fecal coliform bacteria, such as species of Salmonella, could pose a serious health threat in contaminated water resources. Traditional bacterial culture methods and ELISA based assays for identification of fecal coliforms are relatively slow and ambiguous. Polymerase chain reaction of extracted DNA from such bacteria and immunomagnetic separation (IMS) methods appear promising for this application. Although PCR can be a definitive identification technique, it is relatively time consuming when compared to IMS. In this work, the IMS technique has been coupled with an electrochemiluminescence (ECL) technology to separate specific bacteria from their media and quantitatively detect the bacteria within one hour. The sensitivity of the IMS-ECL assay for E.coli O157 strain and Salmonella sp. is as low as 10 - 100 cells/mL in water samples. In addition, IMS was accomplished in dense washings of food and environmental samples followed by ECL assay. These results suggest strongly use of the IMS-ECL methodology for rapid and facile screening of various bacterial contaminations in water resources or other environmental samples for the low level presence of pathogenic coliforms.

  15. In Vitro Monitoring of Total Choline Levels in a Bioartificial Pancreas: 1H NMR Spectroscopic Studies of the Effects of Oxygen Level

    Long, Robert C.; Papas, Klearchos K.; Sambanis, Athanassios; Constantinidis, Ioannis

    2000-09-01

    This investigation implements specifically designed solvent-suppressed adiabatic pulses whose properties make possible the long-term monitoring of 1H NMR detectable metabolites from alginate/poly-l-lysine/alginate (APA)-encapsulated βTC3 cells. Our encapsulated preparations were maintained in a perfusion bioreactor for periods exceeding 30 days. During this prolonged cultivation period, the cells were exposed to repetitive hypoxic episodes of 4 and 24 h. The ratio of the total choline signal (3.20 ppm) to the reference signal (observed at 0.94 ppm assigned to isoleucine, leucine, and valine) decreased by 8-10% for the 4-h and by 20-32% for the 24-h episodes and returned to its prehypoxic level upon reoxygenation. The decrease in the mean value of total choline to reference signal ratio for three 4-h and two 24-h episodes in two different cultures was highly significant (P metabolism are suggested. In addition, the implications of these findings to the development of a noninvasive monitoring method for tissue-engineered constructs composed of encapsulated cells are discussed.

  16. Spirulina platensis effects on the levels of total cholesterol, HDL and triacylglycerols in rabbits fed with a hypercholesterolemic diet

    Luciane Maria Colla

    2008-04-01

    Full Text Available In this work, hypercholesterolemia was induced in rabbits by feeding them a high cholesterol diet (CD, 350 mg/d and the effects of supplementing this diet with 0.5 g/d Spirulina platensis was evaluated by measuring the levels of serum total-cholesterol (TC, triacylglycerols (TAG and high-density lipoprotein (HDL-cholesterol at the start of the experiment and after 30 d and 60 d. It was found that the levels of serum cholesterol decreased from 1,054±101 mg.dL-1 in the rabbits fed a CD without S. platensis to 516±163 mg.dL-1 to those fed with a high cholesterol diet supplemented with S. platensis (significant at p A microalga Spirulina é cultivada e comercializada no mundo devido a suas características nutricionais (elevada concentração de proteínas, em torno de 65%, vitaminas e sais minerais e ao seu potencial terapêutico no tratamento de inúmeras doenças, inclusive a hipercolesterolemia. Neste trabalho foi avaliada a inibição da hipercolesterolemia induzida em coelhos por uma dieta adicionada de colesterol (350 mg.d-1, pela suplementação de 0,5 g.dia-1 de biomassa de Spirulina platensis, sendo avaliados os níveis de colesterol total, triglicerídeos e HDL nos tempos de 0 d, 30 d e 60 d de tratamento. Os resultados indicaram que a adição de Spirulina platensis na dieta ocasionou decréscimo nos níveis de colesterol total de 1054±101 mg.dL-1 para 516±163 mg.dL-1 (p<0,0001, para os coelhos alimentados com a dieta colesterolêmica em comparação com os que receberam a dieta adicionada de Spirulina platensis. A adição de Spirulina a dieta colesterolêmica não ocasionou decréscimo significativo nos níveis de triglicerídios dos coelhos. Os valores de HDL aumentaram de 73±31 mg.dL-1 para 91,0±15,7 mg.dL-1, comparando-se os coelhos alimentados com a dieta colesterolêmica e os alimentados com a dieta adicionada de Spirulina, estatisticamente diferentes a um nível de significância maior que 0,1533.

  17. Estimation of total and bioaccessible levels of iodine in edible seaweeds of Japan by epithermal neutron activation analysis

    Edible seaweeds play important roles as daily source of minerals and dietary fibers for Japanese population groups. It is therefore of interest to estimate the intake of minerals and dietary fibers from seaweeds. Although the levels of iodine can be measured by ICP-MS in liquid samples such as urine and water, it is rather difficult to do the same in seaweeds due to polysaccharides in them. In this study, the following eight edible seaweeds were collected from local shores or purchased from stores. Sea mustard is the most popular edible seaweed in Japan; it starts growing quickly in January until it reaches a height of about 2 m in March. Sea mustards in different growing stages were collected, cut into several longitudinal pieces, freeze dried, and pulverized. Dietary fiber was separated from dried powder by in vitro enzymolysis using α-Amylase, protease, and amyloglucosidase. Portions of dried powder and dietary fiber were irradiated for 1-5 min at the Dalhousie University SLOWPOKE-2 Reactor (DUSR) in Canada or the Kyoto University Reactor (KUR) in Japan. After appropriate cooling time, the 442.9-keV gamma-ray of 128I was used to assay iodine by epithermal instrumental neutron activation analysis (EINAA). Total and bioaccesible iodine levels in eight edible seaweeds are shown

  18. The organic contamination level based on the total soil mass is not a proper index of the soil contamination intensity

    Concentrations of organic contaminants in common productive soils based on the total soil mass give a misleading account of actual contamination effects. This is attributed to the fact that productive soils are essentially water-saturated, with the result that the soil uptake of organic compounds occurs principally by partition into the soil organic matter (SOM). This report illustrates that the soil contamination intensity of a compound is governed by the concentration in the SOM (Com) rather than by the concentration in whole soil (Cs). Supporting data consist of the measured levels and toxicities of many pesticides in soils of widely differing SOM contents and the related levels in in-situ crops that defy explanation by the Cs values. This SOM-based index is timely needed for evaluating the contamination effects of food crops grown in different soils and for establishing a dependable priority ranking for intended remediation of numerous contamination sites. - The concentration of an organic compound in soil based on the entire soil mass is not a proper measure of its contamination intensity.

  19. The seasonality of fecal coliform bacteria pollution and its influence on closures of shellfish harvesting areas in Mississippi Sound.

    Chigbu, Paulinus; Gordon, Scott; Tchounwou, Paul B

    2005-08-01

    Runoff from agricultural lands and farm animal feedlots is one of the major sources of fecal coliforms in surface waters, and fecal coliform (FC) bacteria concentrations tend to vary with season because of seasonal variations in climatic factors. However, El Niño--Southern Oscillation (ENSO) events may affect the extent and patterns of seasonality in FC levels in coastal waters. Water quality monitoring data for shellfish management collected during El Niño (1990, 1992, 1997), and La Niña (1999, 2000) years were analyzed to evaluate the extent to which these events influenced Pearl River stage, and bacterial levels, water temperature, and salinity in the western part of Mississippi Sound. Models to predict FC levels in relation to various environmental factors were also developed. In 1990, 1992 and 1997, FC geometric mean counts peaked in late winter (January/February) reaching 120 MPN (February 1990), 165 MPN (January 1992), and 86 MPN (January 1997), and then decreased considerably during spring and summer (1.2 - 19 MPN). Thereafter, FC abundance increased slightly in fall and early winter (1.9 - 24 MPN). Fecal coliform abundance during the 2000 La Niña year was much lower (1.0 -10.3 MPN) than in 1992 (1.2 - 165 MPN), and showed no seasonal pattern from January to August, perhaps due to the relative scarcity of rainfall in 2000. In 1995 (ENSO neutral year), peak geometric mean FC count (46 MPN) was lower than during El Niño years and occurred in early spring (March). The seasonal and between year variations in FC levels determined the number of days during which the conditionally approved shellfish growing area was opened for harvesting shellfish. For example, from January to April 1997, the area was not opened for shellfish harvesting, whereas in 2000, the number of days during which the area was opened ranged from 6 - 27 (January to April) to 24 - 26 (October to December). ENSO events thus influenced the extent and timing of the peak levels of fecal

  20. The Seasonality of Fecal Coliform Bacteria Pollution and its Influence on Closures of Shellfish Harvesting Areas in Mississippi Sound

    Paul B. Tchounwou

    2005-08-01

    Full Text Available Runoff from agricultural lands and farm animal feedlots is one of the major sources of fecal coliforms in surface waters, and fecal coliform (FC bacteria concentrations tend to vary with season because of seasonal variations in climatic factors. However, El Niño -Southern Oscillation (ENSO events may affect the extent and patterns of seasonality in FC levels in coastal waters. Water quality monitoring data for shellfish management collected during El Niño (1990, 1992, 1997, and La Niña (1999, 2000 years were analyzed to evaluate the extent to which these events influenced Pearl River stage, and bacterial levels, water temperature, and salinity in the western part of Mississippi Sound. Models to predict FC levels in relation to various environmental factors were also developed. In 1990, 1992 and 1997, FC geometric mean counts peaked in late winter (January/February reaching 120 MPN (February 1990, 165 MPN (January 1992, and 86 MPN (January 1997, and then decreased considerably during spring and summer (1.2 – 19 MPN. Thereafter, FC abundance increased slightly in fall and early winter (1.9 – 24 MPN. Fecal coliform abundance during the 2000 La Niña year was much lower (1.0 – 10.3 MPN than in 1992 (1.2 – 165 MPN, and showed no seasonal pattern from January to August, perhaps due to the relative scarcity of rainfall in 2000. In 1995 (ENSO neutral year, peak geometric mean FC count (46 MPN was lower than during El Niño years and occurred in early spring (March. The seasonal and between year variations in FC levels determined the number of days during which the conditionally approved shellfish growing area was opened for harvesting shellfish. For example, from January to April 1997, the area was not opened for shellfish harvesting, whereas in 2000, the number of days during which the area was opened ranged from 6 - 27 (January to April to 24 - 26 (October to December

  1. Assessment of Erythemal UV Level in Nepal Based on Solar UV Estimatks from Total Ozone Mapping Spectrometer

    Binod Kumar BHATTARAF; Berit KJELDSTAD; Trond Morten THORSETH

    2011-01-01

    Nepal lies on the southern slope of Himalaya in Asia. In a width ranging between 150 and 250 km, the altitude varies greatly from about 100 m at its southern border to a maximum of 8848 min the northern part. Like the variation in altitude, climatic condition varies quite a lot. Long-term monthly mean erythemal UV daily dose values for Nepal arc evaluated using Total Ozone Mapping Spectrometer (TOMS) estimation from the time of its overpass between 1996 and 2003. The results are presented as summer and winter maps of mean UV levels in each satellite grid. The mean winter erythemal UV daily dose ranges between 2.1 and 3.6 kJ m-2 whereas summer values are found to lie between 4.6 and 9.7 kJ m -2. The altitude variation increases the UV levels by about 0.2 kJ km-1 in winter months, and 0.9 kJ km-1 in summer. A multiyear monthly average erythemal daily dlose in most of the areas shows that the summer value is about three times higher than that in winter. Although year-to-year variation is not pronounced in high- and mid-elevation regions, UV levels seemed to decrease from 1997 to 2002 in the southern part of the country in the low elevation region by about 5.35%. Due to the combined effects of the altitude, low ozone concentration in the troposphere, and thin air, surface UV radiation at higher altitudes is found to be higher than in the surrounding regions.

  2. Effects of low voltage electrolysis and freezing on coliform content of contaminated water

    A sewage sample was mixed with drinking water and subjected to low voltage (15V) electrolysis in the presence of 1% NaCl. The prepared sample was also kept in freezer with and without the presence of sodium chloride for 4-hours. Among these treatments the electrolysis proved to kill the coliforms, while the freezing reduced the bacterial content. Antibiotics sensitivity patterns revealed that certain of the coliform strains survived the freezing and thawing shocks. Nature of such surviving bacteria and need to study chemical parameters of electrolyzed water are discussed. (author)

  3. Evaluation of some natural products on sugar beet contamined with coliform bacteria group

    Rogéria Maria Alves de Almeida

    2006-08-01

    Full Text Available The sugar beet crop has great importance because its ability on sugar production that can be extracted and crystallized. The use of wastewater in the irrigation has increased because this water has some nutrients sources. However the use of wastewater may cause some health problems due to the presence of coliform bacteria group. In this trial, the objective was to contribute for the sugar beet decontamination. Some products as lemon juice, NaClO and ascetic acid were used by sugar beet immersion during a little time. In conclusion, the lemon juice showed best results by reducing coliform bacteria group.

  4. Characterization of the genomic responses in early Senegalese sole larvae fed diets with different dietary triacylglycerol and total lipids levels.

    Hachero-Cruzado, I; Rodríguez-Rua, A; Román-Padilla, J; Ponce, M; Fernández-Díaz, C; Manchado, M

    2014-12-01

    The aim of this work was to evaluate the genomic responses of premetamorphic sole larvae (9 days post-hatching, dph) fed diets with different lipid and triacylglycerol (TAG) content. For this purpose, two diets with high (rotifers enriched with a fish oil-based emulsion; referred to as HTAG) and low (rotifers enriched with a krill oil-based emulsion; LTAG) levels of total lipids and TAG were evaluated. Lipid class and fatty acid (FA) profiles, histological characterization of intestine, liver and pancreas and expression patterns using RNA-seq were determined. Discriminant analysis results showed that larvae could be clearly differentiated on the basis of their FA profile as a function of the diet supplied until 9dph although no difference in growth was observed. RNA-seq analysis showed that larvae fed HTAG activated coordinately the transcription of apolipoproteins (apob, apoa4, apoc2, apoe, and apobec2) and other related transcripts involved in chylomicron formation, likely to facilitate proper lipid absorption and delivery. In contrast, larvae fed LTAG showed higher mRNA levels of several pancreatic enzymes (try1a, try2, cela1, cela3, cela4, chym1, chym2, amy2a and pnlip) and appetite modulators (agrp1) and some intra- and extracellular lipases. Moreover, KEGG analysis also showed that several transcripts related to lipid metabolism and glycolysis were differentially expressed with a higher abundance in larvae fed LTAG diet. All these data suggest that early larvae were able to establish compensatory mechanisms for energy homeostasis regulating key molecules for FA and TAG biosynthesis, FA uptake and intracellular management of TAG and FA to warrant optimal growth rates. PMID:25463059

  5. Effect of different fibrous and nonfiber carbohydrate levels on nutrients digestibility of total mixed ration using in vivo in buffalo

    Gh. Manafiazar

    2010-02-01

    Full Text Available In order to investigate the effect of different levels of NDF on chewing behavior and their relationship with ruminal acidity, 3 ruminally fistulated buffalo steers (average BW = 389.6 ±7.5 Kg were allotted in a change over design in Agriculture and Natural Resource center of Uremia city of Iran. Two diets with 2 levels of NDF were used as treatments. First and second diets had 52 and 47 % of NDF, respectively. The experiment had two periods and each period’s take 15d for adaptation, 7d for sample collection and determination of nutrient digestibility and for determination of passage rate, 2d for measuring ruminal pH, and 3d for measuring chewing activity. Using Penn Stat Particle Separator (PSPS, distribution, geometric mean, its standard deviation and physically effective factor (pef of diets were determined. Animals fed ad libitum at 09:00a.m and 21:00p.m. The time that spent for eating and rumination were optically recorded in 24h with 5 minutes intervals and total chewing activity calculated from sum of eating and rumination time. There were no significant differences between chemical composition, particles distribution, geometric mean, its standard deviation and physically effective factor (pef of diets, dry matter intake (kg/d and nutrients intake (NDF, ADF, NFC and crude protein and their digestibility, but digestibility ADF, NDF and NFC decreased as content of fiber increased(P1.18 increased (P1.18 increased (P<0.05.

  6. Influencing factors in the occurrence of injured coliforms in the drinking water distribution system in the city of La Plata, Argentina

    Córdoba, Alejandra; Del Coco, V.; Minvielle, Marta Cecilia; Basualdo Farjat, Juan Ángel

    2010-01-01

    The objective of this study was to evaluate the influencing factors in the occurrence of coliforms in the drinking water in La Plata (Argentina) from June 1999 to June 2001. A total of 180 samples were collected from Rio de La Plata (102 samples) and Puelche Aquifer (78 samples); 45 samples were collected for each of the four seasons. The membrane filter procedure was used for isolating bacteria, and each sample was tested for chlorine and pH. The highest percentage of samples positive for co...

  7. Correlation between plasma total nitric oxide levels and cerebral vasospasm and clinical outcome in patients with aneurysmal subarachnoid hemorrhage in Indian population

    Shruthi Shimoga Ramesh

    2014-01-01

    Full Text Available Context: Cerebral vasospasm remains a major cause of morbidity and mortality in patients with aneurysmal subarachnoid hemorrhage (aSAH. Reduced bioavailability of nitric oxide has been associated with the development of cerebral vasospasm after aSAH. Such data is not available in Indian population. Aims: The objective of the study was to measure the plasma total nitric oxide (nitrite and nitrate-NOx level in aSAH patients and healthy controls treated at a tertiary hospital in India and to investigate a possible association between plasma total nitric oxide level and cerebral vasospasm and clinical outcome following treatment in patients with aSAH. Settings and Design: A case-control study of aSAH patients was conducted. Plasma total NOx levels were estimated in aSAH patients with and without vasospasm and compared the results with NOx levels in healthy individuals. Materials and Methods: aSAH in patients was diagnosed on the basis of clinical and neuro-imaging findings. Plasma total NOx levels in different subject groups were determined by Griess assay. Results: Plasma total NOx level was found to be significantly decreased in patients with aSAH when compared to controls. Plasma total NOx level in the poor-grade SAH group was lower than that in the good-grade SAH group. Plasma total NOx level further reduced in patients with angiographic (P < 0.05 and clinical vasospasm. Conclusions: Reduced plasma NOx level is seen in aSAH patients as compared to normal individuals. In aSAH patients reduced levels are associated with increased incidence of cerebral vasospasm and poor outcome. Plasma total NOx level could be used as a candidate biomarker for predicting vasospasm and outcome for this pathology.

  8. Serum levels of free and total insulin-link growth factor (IGF)-1 and (IGF) binding protein-3 in normal and growth hormone deficient children

    Serum levels of total insulin-like growth factor- 1 (IGF-1) and IGF-binding protein-3 (IGFBP-3) reflect endogenous GH secretion in healthy children, which makes them good diagnostic markers for screening GH deficiency (GHD) in short children, although some controversy still exists. Only a minor fraction of the total IGF-1 circulates in its free form, which is believed to be the biologically active form. Serum levels of free IGF-1, total IGF-I and IGFBP-3 were measured in 144 healthy children (72 boys and 72 girls, aged from 0 to 16 years) and in 12 prepubertal GH. deficient (GHD) children to study correlation between the age and free IGF-1, total IGF-1 and IGFBP-3 levels. In healthy subjects (both sexes), serum free IGF-1, total IGF-1 and IGFBP-3 levels were low in infancy, increasing during puberty and declining thereafter. Free IGF-1 in serum occupied about 0.97. 1.45 % of the total IGF-1 values, and the ratios of free IGF-1 to total IGF-1 were significantly increased in the pubertal age groups than in the prepubertal age groups. Serum levels of free IGF-1 showed significant positive correlation with those of total IGF-I and IGFBP-3. Serum free IGF-1, total IGF-1 and IGFBP-3 levels in patients with GHD decreased significantly with increasing degree of hypopituitarism. These observations suggest that the increase in serum free IGF-1 level during puberty was caused by a dramatic increase in total IGF-1 rather than IGFBP-3. Also, high levels of these hormones may play an important role in pubertal growth spurt and may become a useful tool for diagnosing GHD and predicting growth response to long term GH therapy

  9. Diets formulated on total or digestible amino acid basis with different energy levels and physical form on broiler performance

    A Maiorka

    2005-03-01

    Full Text Available We studied the effects of two different systems of expressing amino acid requirements (total - TAA or digestible - DAA, two energy levels (2,900 or 3,200 kcal of ME/kg and two physical forms (mash or pellet on the performance of male broilers from 21 to 42 d of age. Diets formulated on DAA basis improved feed conversion (p<0.001 and the conversion of consumed ME into body weight gain (p<0.001. Birds fed 3,200 kcal ME/kg presented better feed conversion (p<0.001, higher abdominal fat deposition (p<0.001 and worse conversion of consumed ME into body weight gain (p<0.001 as compared to birds fed 2,900 kcal ME/kg. Birds fed pelleted feed had higher feed intake (p<0.001, higher weight gain (p<0.001, better feed conversion (p<0.001, better conversion of consumed ME into body weight gain (p<0.001 and higher abdominal fat deposition (p<0.001. The results obtained in the present study suggest that feed formulation based on DAA is required when diets contain protein sources which amino acid digestibility is unreliable. Besides, pelleted feed improves performance parameters of broilers.

  10. Posibilidad de identificar defectos en máquinas rotatorias utilizando la medición de nivel total de la señal vibratoria // Possibility of identification of defects in rotatory machinery by means of total level mesurations.

    L. M. Véliz Marrero

    2000-03-01

    Full Text Available Se expone una herramienta propuesta por el Dr. C. Cempel, poco difundida para identificar algunos defectos al realizar eldiagnóstico de máquinas rotatorias utilizando la medición de Nivel Total. Se ilustra a modo de ejemplo el diagnóstico de unreductor de velocidad.Palabras claves: Medición, vibraciones, nivel total, diagnóstico, engranaje, reductor de velocidad.________________________________________________________________________________AbstractIn this paper is exposed a not much divulged tool proposed by Dr. C. Cempel for the identification of some defects by meansof the measurement of Total Level of vibration in diagnosis of rotatory machinery. As example, a speed reducer diagnostic ispresented.Key words: Vibration measurement, total level, diagnostic, gears, speed reducer.

  11. Serum total cholesterol level and some cancer mortality:A 7 years follow-up study in 698796 people

    ZHANG Qin-feng; WU Duo-wen; Jong Ku Park; Sang Baek Koh; Chun Bae Kim; Sei Jin Chan

    2003-01-01

    were lung cancer during the follow up period. Meanwhile serum total cholesterol levels were inversely and significantly related to overall cancer and liver cancer mortality in population, in both male and female. Overall cancer and liver cancer mortality decreased with increasing cholesterol levels at the lowest levels in both gender. This effect is still apparent when deaths within three years are excluded in population and male. A weak inverse association between cholesterol and stomach cancer. This is not significant for cholesterol and lung cancer. Conclusion: There is important relation between lower serum cholesterol increased the risk of overall cancer and liver cancer mortality. Low serum cholesterol concentration as predictor of overall cancer and liver cancer is importance in both genders.

  12. Method of Detecting Coliform Bacteria and Escherichia Coli Bacteria from Reflected Light

    Vincent, Robert (Inventor)

    2013-01-01

    The present invention relates to a method of detecting coliform bacteria in water from reflected light and a method of detecting Eschericha Coli bacteria in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.

  13. Fast detection of coliform bacteria by means of gas chromatography-differential mobility spectrometry.

    Saptalena, Lena Ganda; Kuklya, Andriy; Telgheder, Ursula

    2016-05-01

    In this study, we demonstrate that the combination of an enzymatic method (based on Colilert-18 medium) and gas chromatography-differential mobility spectrometry (GC-DMS) can reduce the time required for detection of coliform bacteria (including Escherichia coli) from 18 to 2.5 h. The presented method includes the incubation (~2.5 h) of the sample containing coliform bacteria in Colilert-18 medium. The incubation time of 2.5 h is required for the activation of the β-galactosidase enzyme. Produced during the incubation biomarker o-nitrophenol (ONP) can be detected by means of GC-DMS within just 200 s. The detection limit for ONP was 45 ng (on-column). The method developed in this work provides significantly shorter analysis time compared with standard methods, and can be potentially adapted to the field conditions. Therefore, this method is a promising tool for an early detection of coliform bacteria (including E. coli). Graphical Abstract Fast detection of coliform bacteria by means of GC-DMS. PMID:27002609

  14. NEW MEDIUM FOR IMPROVED RECOVERY OF COLIFORM BACTERIA FROM DRINKING WATER

    A new membrane filter medium was developed for the improved recovery of injured coliforms from drinking water. The new medium, termed m-T7, consists of 5.0 g of Difco Proteose Peptone no. 3,20 g of lactose, 3.0 g of yeast extract, 0.4 ml of Tergitol 7 (25% solution), 5.0 g of pol...

  15. Contamination of faecal coliforms in ice cubes sampled from food outlets in Kubang Kerian, Kelantan.

    Noor Izani, N J; Zulaikha, A R; Mohamad Noor, M R; Amri, M A; Mahat, N A

    2012-03-01

    The use of ice cubes in beverages is common among patrons of food outlets in Malaysia although its safety for human consumption remains unclear. Hence, this study was designed to determine the presence of faecal coliforms and several useful water physicochemical parameters viz. free residual chlorine concentration, turbidity and pH in ice cubes from 30 randomly selected food outlets in Kubang Kerian, Kelantan. Faecal coliforms were found in ice cubes in 16 (53%) food outlets ranging between 1 CFU/100mL to >50 CFU/ 100mL, while in the remaining 14 (47%) food outlets, in samples of tap water as well as in commercially bottled drinking water, faecal coliforms were not detected. The highest faecal coliform counts of >50 CFU/100mL were observed in 3 (10%) food outlets followed by 11-50 CFU/100mL and 1-10 CFU/100mL in 7 (23%) and 6 (20%) food outlets, respectively. All samples recorded low free residual chlorine concentration (food outlets or (2) they may not be the water sources used for making ice cubes. Since low free residual chlorine concentrations were observed (food outlets, including that of ice cube is crucial in ensuring better food and water for human consumption. PMID:22543605

  16. Comparison of Selected Methods for the Enumeration of Fecal Coliforms and Escherichia coli in Shellfish

    Grabow, W. O. K.; De Villiers, J C; Schildhauer, C. I.

    1992-01-01

    In a comparison of five selected methods for the enumeration of fecal coliforms and Escherichia coli in naturally contaminated and sewage-seeded mussels (Choromytilus spp.) and oysters (Ostrea spp.), a spread-plate procedure with mFC agar without rosolic acid and preincubation proved the method of choice for routine quality assessment.

  17. Coliformes em água de abastecimento de lojas fast-food da Região Metropolitana de Recife (PE, Brasil Coliforms in the water supply of fast-food chains in the Metropolitan Region of Recife, in the state of Pernambuco (Brazil

    Maria Anunciada Leal Porto

    2011-05-01

    Full Text Available A garantia do fornecimento de água potável nos serviços de alimentação é uma questão relevante para a saúde pública. Assim, o objetivo deste estudo foi avaliar a qualidade microbiológica da água destinada ao abastecimento de uma rede de lojas fast-food da cidade de Recife (PE e Região Metropolitana e comparar os resultados aos padrões estabelecidos pela Portaria nº 518/2004 do Ministério da Saúde. Mensalmente, foi analisada uma amostra proveniente de uma das torneiras da área de manipulação das oito lojas investigadas, perfazendo 96 amostras ao longo de um ano, todas coletadas em duplicata. As análises seguiram a metodologia estabelecida na American Public Health Association (APHA para realização do ensaio presuntivo utilizando o Teste Presença-Ausência, considerando-se o padrão de potabilidade determinado na legislação pertinente. Os resultados revelaram que 11,46% de todas as amostras apresentaram água contaminada por coliformes totais e 1,04% contaminação por coliformes termotolerantes. Conclui-se, portanto, que a qualidade da água disponível nos estabelecimentos produtores de alimentos estudados encontra-se em estado de alerta, uma vez que o percentual significativo das amostras analisadas mostrava-se impróprio para o consumo humano de acordo com a legislação vigente, a qual preconiza ausência de coliformes totais e termotolerantes.A guaranteed supply of clean drinking water in food outlets is a relevant subject for public health. The scope of this study was to assess the microbiological quality of 96 water samples of a network of fast-food stores in the city of Recife (state of Pernambuco, Brazil and Metropolitan Area and to compare the results to the standards established by Brazilian Health Ministry decree nº 518/2004. Every month, a double sample from one of the faucets in the food preparation area of the eight stores investigated was analyzed, totaling 96 samples over one year. The analyses followed

  18. Serum total IgE levels and total eosinophil counts: relationship with treatment response in patients with acute asthma Relação dos níveis séricos de IgE total e das contagens de eosinófilos com a resposta ao tratamento em pacientes com asma aguda

    Ebrahim Razi; Gholam Abbass Moosavi

    2010-01-01

    OBJECTIVE: To determine whether serum total IgE levels and total eosinophil counts have any relationship with the response to routine pharmacological treatment in patients with acute asthma. METHODS: A cross-sectional study involving 162 patients with acute asthma. Serum total IgE levels, peripheral blood cell counts and eosinophil counts were determined. The treatment was adjusted for each patient according to the severity of asthma. Spirometry was performed at baseline and two weeks after t...

  19. COLIFORMES TERMOTOLERANTES EN AGUAS DE LAS POBLACIONES COSTERAS Y PALAFÍTICAS DE LA CIÉNAGA GRANDE DE SANTA MARTA, COLOMBIA Fecals Coliforms in Waters of Coastal and Palafiticos Populations of Ciénaga Grande de Santa Marta, Colombia

    SILVIA NARVÁEZ

    palafitics populations (Buenavista, Nueva Venecia and Trojas de Cataca during the period 2004-2007. Additionally, pH, temperature, salinity and oxygen measurements were carried out in each station. The highest coliforms concentrations were found in Trojas de Cataca, in the dry season more each year (7,800-9,000 MPN/100ml and the lowest level in Buenavista station (2-1,100 MPN/100mL. The behavior pattern of Coliform densities in coastal populations was similar in the time and there was no direct relationship between physicochemical variables and Coliform group. It is important take care and watch over the estuary quality, taking into account that constitutes a potential risk source for economic activities development of the area and for the residents health.

  20. Intact and total insulin-like growth factor-binding protein-3 (IGFBP-3) levels in relation to breast cancer risk factors: a cross-sectional study

    Diorio, Caroline; Brisson, Jacques; Bérubé, Sylvie; Pollak, Michael

    2008-01-01

    Introduction Levels of insulin-like growth factor (IGF)-I and its main binding protein (IGFBP-3) have been associated with breast cancer risk among premenopausal women. However, associations of IGFBP-3 levels with breast cancer risk have been inconsistent, possibly due to the different predominant forms of circulating IGFBP-3 (intact versus fragmented) that were measured in these studies. Here, we examine the association of breast cancer risk factors with intact and total IGFBP-3 levels. Methods This cross-sectional study includes 737 premenopausal women recruited at screening mammography. Plasma intact and total IGFBP-3 and IGF-I levels were measured by enzyme-linked immunosorbent assay methods. Percent and absolute breast density were estimated using a computer-assisted method. The associations were evaluated using generalized linear models and Pearson (r) or Spearman (rs) partial correlation coefficients. Results Means ± standard deviations of intact and total IGFBP-3 levels (ng/mL) were 1,044 ± 234 and 4,806 ± 910, respectively. Intact and total IGFBP-3 levels were correlated with age and smoking. Levels of intact IGFBP-3 were negatively correlated with waist-to-hip ratio (WHR) (r = -0.128; P = 0.0005), parity (rs = -0.078; P = 0.04), and alcohol intake (r = -0.137; P = 0.0002) and positively correlated with energy intake (r = 0.075; P = 0.04). In contrast, total IGFBP-3 levels were positively correlated with WHR (r = 0.115; P = 0.002), parity (rs = 0.089; P = 0.02), body mass index (BMI) (r = 0.115; P = 0.002), physical activity (r = 0.118; P = 0.002), and IGF-I levels (r = 0.588; P total (intact and fragmented) IGFBP-3 levels. These findings suggest that different molecular forms of IGFBP-3 may bear different relations to premenopausal breast cancer risk. PMID:18471292

  1. Higher Total Serum Cholesterol Levels Are Associated With Less Severe Strokes and Lower All-Cause Mortality

    Olsen, Tom Skyhøj; Christensen, Rune Haubo Bojesen; Kammersgaard, Lars;

    2007-01-01

    Background and Purpose - Evidence of a causal relation between serum cholesterol and stroke is inconsistent. We investigated the relation between total serum cholesterol and both stroke severity and poststroke mortality to test the hypothesis that hyperch....

  2. A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage

    Willy Jablonka; Raquel Senna; Thaisa Nahu; Guilherme Ventura; Lidiane Menezes; Silva-Neto, Mário Alberto C.

    2011-01-01

    Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen in extracts prepared from both male and female ...

  3. Combined Effect of heat stress and radiation exposure on levels of testosterone lactate dehydrogenase, total lipids and cholesterol in blood plasma and testicular tissue of male albino rats

    Hyperthermia and radiotherapy are used as relatively successful modalities in the treatment of some diseases. This work aims to gain a better understanding about the testicular physiological changes caused by a combined stress of heat and gamma radiation. Rats were divided into four groups : 1.Control, 2.Irradiated at a dose level 6 Gy, 3.Exposed to heat tress for one, 3, 5 and 7 days and group 4 exposed to heat tress as group 3 followed by irradiation directly at the end of heat treatment. Plasma levels of testosterone, lactate dehydrogenase (LDH), total lipids and total cholesterol, as well as, testicular content of total lipids and total cholesterol were detected. Results showed that irradiation (6 Gy) induced significant decreases (P<0.001) in plasma testosterone level, whereas, it elevated significantly the plasma levels of LDH activity and total cholesterol. The heat stress caused significant declines in plasma testosterone , LDH (after 1, 3 and 5 days) and total lipids, but not testicular content of total lipids. Conversely, it increased the rectal temperature and both, plasma and testicular tissue content of total cholesterol levels. The whole-body gamma irradiation direcrly post heat stress intervals boosted the heat stress effect on all the examined parameters. Hence it could be concluded that exposure to whole-body gamma irradiation in high ambient temperatures diminished the testicular function by decreasing the sexual hormone testosterone although its precursor cholesterol increased in both plasma and testicular tissue. This combined treatment seemed to block the mechanism of testosterone formation in testicular tissue, and in turn, decresed its levels in plasma. Further investigations are needed to study such combined effect of heat stress andirradiation on the testicular potency and the testicular function

  4. Serum total IGF-I, free IGF-I, and IGFBP-1 levels in an elderly population - Relation to cardiovascular risk factors and disease

    Janssen, JAMJL; Stolk, RP; Pols, HAP; Grobbee, DE; Lamberts, SWJ

    1998-01-01

    Recently, a method to measure free insulin-like growth factor-I (IGF-I) levels has been developed. Free IGF-I levels may have greater physiological and clinical relevance than total (bound and free) IGF-I. The associations between the circulating IGF-I/IGF binding protein (IGFBP) system and cardiova

  5. Influence of Ileo-Caecal Cannulation and Oxytetracycline on Ileo-Caecal and Rectal Coliform Populations in Pigs

    Wallgren P; Lindberg JE; Högberg A

    2001-01-01

    The effect of surgery (insertion of an ileo-caecal cannula) and a subsequent parenteral treatment with oxytetracycline on the ileo-caecal and rectal coliform populations in 7 Swedish Yorkshire castrates were studied. Samples were collected during surgery as well as 3, 7, 14 and 20 days post surgery. The diversity of the enteric coliform flora was initially high both in the ileo-caecal ostium and in rectum. No alteration in the diversity of the enteric coliform flora was observed following su...

  6. Presence Of Multi Drug Resistant Coliform Bacteria Isolated From Biofilm Of Sachet And Borehole Waters Sold In Abakaliki Metropolis Ebonyi State Nigeria.

    Okafor Collins Onyebuchi Okeke

    2015-06-01

    Full Text Available ABSTRACT This study investigated the presence of multi drug resistant coliform bacteria from biofilm of sachet and borehole waters sold in Abakaliki metropolis in Ebonyi State Nigeria. Five hundred 500 samples of water comprising 250 each from selected brand of sachet water retailers and borehole water dispensers from seven locations were sampled for the detection of coliform bacteria from biofilm and to determine their antimicrobial susceptibility using commercially prepared antibiotic discs. Results revealed a high faecal contamination level in sachet waters as Gospel 36 72 Aqua Rapha 30 60 and Bejoy 18 36 were the highest among the sachet water brands examined with Nene and Rock Tama sachet water brands having the lowest contamination level of 612 and 1326 respectively. Borehole samples results revealed that Aboffia had 27 76.93 samples contaminated with faecal bacteria while Azugwu 11 28.5 Azuiyiokwu 18 50 Azuiyiudene 2980 Kpirikpiri 24 66.63 PrescoNtezi 1646.15 and Udensi 22 61.54. Escherichia coli Enterobacter spp and Klebsiella spp were the major contaminants of both sachet and borehole water samples. The bacteria isolates from biofilm of sachet and borehole waters were susceptible to only three of the antibiotics used namely nitrofurantoin amoxycilin and ampicillin. The bacteria were completely resistant to ciprofloxacin tetracycline norbactinnorfloxacin ofloxacin cefuroxime and gentamicin. This showed that they exhibit multi-drug resistance pattern which is a common feature of medically important biofilm bacteria. We therefore report the presence of multi-drug resistant coliform bacteria from biofilm of sachet and borehole waters sold in Abakaliki metropolis Ebonyi State Nigeria.

  7. Técnicas de análisis de tendencias en mediciones espectrales y de nivel total de parámetros de diagnóstico // Trend analysis techniques in spectral and total level mensurations

    L. Penabad Sanz

    2000-01-01

    Full Text Available En el presente artículo se muestra la implementación del análisis de tendencia, como tecnología predictiva, a través de laestadística matemática y su utilización en mediciones de parámetros de diagnóstico tanto espectral como de nivel total. Elempleo de esta tecnología resulta de gran importancia debido a que permite pronosticar el momento de fallo, según elcomportamiento de los niveles de los parámetros medidos, lo cual facilita la gestión del mantenimiento.Palabras claves: mantenimiento, análisis de tendencia, medición espectral, medición de nivel total, pronósticode fallo, vibraciones.______________________________________________________________________AbstractIn this article is shown the implementation of the trend analysis, as predictive technology, through the mathematical statisticand its use in spectral and total level mensurations of diagnosis parameters. The use of this technology has a great importancebecause it allows to predict the failure moment, according to the behavior of the levels of measured parameters, that whichfacilitates the administration of the maintenance.Key words: maintenance, tendency analysis, spectral mensuration, total level mensuration, failure presage,vibrations, diagnosis.

  8. The Relationship Between Serum Levels of Total IgE, IL-18, IL-12, IFN- γ and Disease Severity in Children With Atopic Dermatitis

    2006-01-01

    Full Text Available Studies about the role of cytokines on the immunopathogenesis of atopic dermatitis (AD are generally based on in vitro observations and this role has not been completely clarified yet. Serum levels of total IgE, IL-18, IL-12, IFN- γ and the relationship between these parameters and disease severity, determined using the SCORAD index, in a group of atopic patients were investigated in this study. Serum levels of total IgE were measured by the nephelometric method and serum levels of IL-18, IL-12/p40 and IFN- γ were measured by ELISA method. Serum levels of total IgE and IL-18 were found significantly higher in study group than in controls ( p<.001 . There was no statistically significant difference between patients and controls in respect of serum levels of IL-12/p40 ( p=.227 . A statistically significant relationship between SCORAD values and serum levels of total IgE ( p<.001 , IL-18 ( p<.001 , and IL-12/p40 ( p<.001 was determined. These results show that serum levels of IL-18 can be a sensitive parameter that importantly correlates with clinical severity of AD, can play a role in the immunopathogenesis of AD, and furthermore may be used in the diagnosis and follow-up of the disease in addition to other parameters.

  9. Influence of pH and Oxidant Ozone to Amount of Bacterium Coliform at Hospital Waste

    Influence of pH and oxidant ozone to amount of bacterium coliform at hospital waste have been done. As sample is liquid waste Public Hospital of town (RSUD) Yogyakarta. Sample waste processed by 3 kinds of treatment, that is first certain ozone waste during, that is waste given by the third and just chalk of waste given by the certain and ozonization chalk during. From third the treatment, in the reality third treatment which can give the maximal result, that is waste given the chalk until pH waste 8.5 and ozonization during 40 minute give the following result : bacterium coliform from 810.000 MPN become 0 MPN ( cell / 100 mL). This result have fulfilled the conditions as according to decision of Governor of DIY no. 65 year 1999 for the waste of faction II, that is waste used for the irrigation of fishery and agriculture. (author)

  10. Factors associated with marketable milk production recovery after treatment of naturally occurring acute coliform mastitis.

    Shinozuka, Yasunori; Kaneko, Sohei; Kurose, Tomoyasu; Watanabe, Aiko; Kuruhara, Kana; Kawai, Kazuhiro

    2016-06-01

    Milk production loss after recovery from acute coliform mastitis causes major economic losses for dairy industries. Declines in milk production and composition are caused by multiple factors, including cow factors, microorganisms and treatments, but the influence of each factor has not been determined. To investigate risk factors for milk loss after treatment for acute coliform mastitis, multiple logistic regression analyses were conducted in 53 clinical cases. Systemic administration of fluoroquinolone was significantly associated with recovery of marketable milk production. The time to slaughter was significantly shorter in cows with complete loss of quarter milk production than in cows that produced marketable milk. In this study, we identified factors associated with increased risk of milk production loss. PMID:26860356

  11. Contribution of thermolabile methylenetetrahydrofolate reductase variant to total plasma homocysteine levels in healthy men and women. Inter99 (2)

    Husemoen, Lise Lotte N; Thomsen, Troels F; Fenger, Mogens;

    2003-01-01

    Elevation in plasma total homocysteine (tHcy) is believed to be causally related to cardiovascular disease. Like age and sex, the thermolabile variant of methylenetetrahydrofolate reductase (MTHFR(C677T)) is an important nonmodifiable determinant of tHcy, which may be considered when describing...

  12. Influence of anthropogenic activities on microbial and nutrient levels along the Mara River tributaries, Kenya

    Douglas Nyambane Anyona

    2014-05-01

    Full Text Available Background: A number of factors have a negative impact on natural surface water resources across the world. Although sources of surface water pollution are numerous, anthropogenic activities have been singled out as among the most important and of great concern. The aim of this study was to assess the influence of anthropogenic activities on nutrients and microbial levels along the Amala and Nyangores tributaries of the Mara River in Kenya. Materials and Methods: Four sampling sites along each tributary were specifically selected from which water samples were collected and analyzed for nutrients by use of spectrophotometric techniques, and coliform bacterial presence by a multiple tube fermentation technique. Results: Higher levels of total phosphorus were recorded along the Nyangores than the Amala tributary (P= 0.02. Significant differences in phosphorus levels were recorded between different sites along the Nyangores tributary (P=<0.001 and also along the Amala tributary (P= 0.0036. However, total nitrogen levels varied only within sites along the Nyangores tributary (P<0.0001 but not along the Amala tributary. Similarly, Escherichia coli and total coliform levels varied significantly within Nyangores tributary sites. Sites with frequent and direct human and livestock contact had higher microbial and nutrient levels, indicative of a localized pollution effect. Conclusion: The findings imply that the health of local communities who depend on this water for domestic use might be compromised. As such, regular monitoring, strict enforcement of environmental protection laws, public education and proper sewage disposal is recommended.

  13. High milk neutrophil chemiluminescence limits the severity of bovine coliform mastitis

    Mehrzad, Jalil; Duchateau, Luc; Burvenich, Christian

    2005-01-01

    Polymorphonuclear neutrophil (PMN) function changes during mastitis. To investigate the contribution of milk PMN to the severity of Escherichia coli (E. coli) mastitis, chemiluminescence ( CL) of blood and milk PMN and their efficiency to destroy coliform bacteria in the mammary gland were examined following the induction of E. coli mastitis in early lactating cows. To better assess and define the degree of mastitis severity, cows were classified as moderate and severe responders according to...

  14. Prevention of clinical coliform mastitis in dairy cows by a mutant Escherichia coli vaccine.

    González, R N; Cullor, J S; Jasper, D. E.; Farver, T B; Bushnell, R B; Oliver, M N

    1989-01-01

    A prospective cohort study was undertaken in two commercial California dairies. The treatment group, 246 cows, received three doses of a whole cell bacterin of J5 Escherichia coli (mutant of E. coli O111:B4) plus Freund's incomplete adjuvant vaccine (two in the dry period and one after calving) while 240 unvaccinated cows served as controls. Thirty-five cases of clinical coliform mastitis were diagnosed, six in vaccinated cows and 29 in unvaccinated cows. Bacteria isolated from the clinical c...

  15. THAWING PROCEDURES FOR HOSPITAL-MADE ENTERAL FEEDINGS: SURVIVAL OF COLIFORM AND MESOPHILIC AEROBIC BACTERIA

    KATHIA ROSSI ROLIM LOPES; IZABEL BRANDãO STREIT; ROSA HELENA LUCHESE; MARCO ANTôNIO ZACHIA AYUB

    2009-01-01

    ABSTRACT: This study had the purpose to observe the effect of thawing procedures on survival of coliform and mesophilic aerobic bacteria in hospital-made enteral feedings. The samples are represented by three different lots. The tests were realized in three moments: immediately after the sample preparation and after freezing during 1 or 2 months. The thawing procedures were denominated convencional and alternative. The first, used by hospital, utilizes water bath at 5...

  16. Effect of oral administration of Bacillus coagulans B37 and Bacillus pumilus B9 strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model

    Lopamudra Haldar

    2016-07-01

    Full Text Available Aim: To investigate the effect of oral administration of two Bacillus strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model. Materials and Methods: An in vivo experiment was conducted for 49-day period on 36 adult male albino Wister rats divided equally into to four groups. After 7-day adaptation period, one group (T1 was fed on sterile skim milk along with basal diet for the next 28 days. Second (T2 and (T3 groups received spore biomass of Bacillus coagulans B37 and Bacillus pumilus B9, respectively, suspended in sterilized skim milk at 8-9 log colony-forming units/ml plus basal diet for 28 days, while control group (T4 was supplied with clean water along with basal diet. There was a 14-day post-treatment period. A total of 288 fecal samples (8 fecal collections per rat were collected at every 7-day interval starting from 0 to 49 days and subjected to the enumeration of the counts of coliforms and lactobacilli and Bacillus spores using respective agar media. In vitro acid and bile tolerance tests on both the strains were performed. Results: The rats those (T2 and T3 received either B. coagulans B37 or B. pumilus B9 spore along with non-fermented skim milk showed decrease (p<0.01 in fecal coliform counts and increase (p<0.05 in both fecal lactobacilli and Bacillus spore counts as compared to the control group (T4 and the group fed only skim milk (T1. In vitro study indicated that both the strains were found to survive at pH 2.0 and 3.0 even up to 3 h and tolerate bile up to 2.0% concentration even after 12 h of exposure. Conclusions: This study revealed that oral administration of either B. coagulans B37 or B. pumilus B9 strains might be useful in reducing coliform counts accompanied by concurrent increase in lactobacilli counts in the intestinal flora in rats.

  17. Total and free serum haloperidol levels in schizophrenic patients and the effect of age thioridazine and fatty acid on haloperidol-serum protein binding in vitro.

    Rowell, F. J.; Hui, S M; Fairbairn, A F; Eccleston, D

    1981-01-01

    1 Using radioimmunoassays, steady-state levels of total and free haloperidol (obtained by ultrafiltration and dialysis) have been determined in twenty-two patients on long term treatment receiving doses from 3 to 45 mg per day. 2 For the group, both total serum concentration and free drug concentration showed significant correlations (P less than 0.001) with daily dose. 3 No significant correlation was observed between age of the patient and percentage free haloperidol in serum. 4 In vitro ex...

  18. Circulating High-Molecular-Weight (HMW) Adiponectin Level Is Related with Breast Cancer Risk Better than Total Adiponectin: A Case-Control Study

    Ming-ming Guo; Xue-ning Duan; Shu-de Cui; Fu-guo Tian; Xu-chen Cao; Cui-zhi Geng; Zhi-min Fan; Xiang Wang; Shu Wang; Hong-chuan Jiang; Jian-guo Zhang; Feng Jin; Jin-hai Tang; Hong Liang; Zhen-lin Yang

    2015-01-01

    The level of total adiponectin, a mixture of different adiponectin forms, has been reported associated with breast cancer risk with inconsistent results. Whether the different forms play different roles in breast cancer risk prediction is unclear. To examine this, we measured total and high molecular weight (HMW) adiponectin in a case-control study (1167 sets). Higher circulating HMW adiponectin was negatively associated with breast cancer risk after adjusting for menopausal status and family...

  19. Monitoring and spatial distribution of heterotrophic bacteria and fecal coliforms in the Rodrigo de Freitas Lagoon, Rio de Janeiro, Brazil

    Lutterbach Márcia T. S.

    2001-01-01

    Full Text Available The distribution of heterothrophic bacteria and fecal coliforms was monitored at four sampling stations located near the shore of the Rodrigo de Freitas Lagoon, in the city of Rio de Janeiro, Brazil. Water samples were collected, monthly from October 1994 through September 1998. The highest heterothrophic count (6.5x10 7 CFU/100mL was recorded at stations 2 and 4 during August 1998 and the lowest (10 ³ CFU/100 mL at station 3 during February 1995. With respect to fecal coliforms, the highest and lowest counts were 1.6x10 5 coliforms/100mL at station 3 during March 1997 and <1 coliform/100mL at all the stations during February 1995 and September 1997 as well as station 3 during February 1998. The data indicated a percentage increase of the microorganisms surveyed over time at all the sampling stations studied.

  20. The relationship between debt levels and total shareholder return of JSE-listed platinum companies / Sandra Jooste

    Jooste, Sandra

    2015-01-01

    Investors make investment decisions based on their risk appetite. Furthermore, when such investors consider shares as part of their investment portfolio, these investors will consider the risk profile of the company it is interested in. By taking on a certain level of risk, shareholders expect to be commensurately compensated. Shareholders of companies with relatively higher debt levels in their capital structure and therefore higher financial risk, require a relatively higher ...

  1. Extra-intestinal symptoms in patients with irritable bowel syndrome: related to high total IgE levels and atopic sensitization?

    Vara, Ellen Johanne; Valeur, Jørgen; Hausken, Trygve; Lied, Gülen Arslan

    2016-08-01

    Objective We have previously found that high levels of total IgE, but not atopic sensitization, was a significant predictor for functional gastrointestinal (GI) symptoms. In this study, we aimed to assess the prevalence of extra-intestinal symptoms in IBS patients, and explore their relation to total IgE levels and atopic sensitization. Materials and methods Seventy-one patients with functional GI complaints were included. Severity of GI symptoms, fatigue and musculoskeletal pain was evaluated using the following questionnaires: IBS-Severity Scoring System (IBS-SSS), Fatigue Impact Scale (FIS), FibroFatigue Scale (FFS), and Visual Analog Scales (VAS) for musculoskeletal pain. Levels of total IgE and specific IgE-antibodies were analyzed. Results Fatigue and musculoskeletal pain were demonstrated in 78.9 and 43.7% of the patients, respectively. IBS-SSS scores were significantly correlated with fatigue scores and musculoskeletal pain. Patients with fatigue and musculoskeletal pain had significantly higher IBS-SSS scores than patients without fatigue and musculoskeletal pain. Total IgE levels were significantly higher in IBS patients compared to a healthy control group from a previous study. However, neither total IgE nor atopic sensitization was significantly associated with extra-intestinal symptoms. Conclusions IBS, fatigue, and musculoskeletal pain were significantly associated. Total IgE levels were higher in IBS patients than healthy controls, but not related to intestinal or extra-intestinal symptom severity. Atopy was not associated with any of the co-morbidities. Thus, the clinical significance of high IgE levels in IBS remains unclear and further studies are warranted to explore a common underlying mechanism for the co-morbid triad of IBS, fatigue, and musculoskeletal pain. PMID:27161383

  2. Diagnostic values of combination of free running asthma screening test and total serum allergen IgE level in children with asthma

    Chen Xin; Li Yong; Zeng Meiying

    2014-01-01

    Background A free running asthma screening test is usually used for screening exercise-induced bronchoconstriction.The total serum allergen IgE level can reveal the patient's atopy characteristics.Our study is to evaluate the diagnostic values of the combination of the two tests in asthmatic children and compare this new diagnostic method with the Intemational Study of Asthma and Allergies in Childhood (ISSAC) questionnaires and the bronchial provocation test,which are popular diagnostic tool for pediatric asthma.Methods A total of 773 school children were recruited in this study.The children's asthma was diagnosed by means of a combination of the free running asthma screening test and total serum allergen IgE level.The new diagnostic method value was assessed using receiver operating characteristic (ROC) curves and compared with other diagnostic tools such as ISSAC questionnaires and the bronchial provocation test.Results The AUC of this new diagnostic method was higher than 0.9.When the cut-off value of total serum allergen IgE level was ≥47 KU/L,the sensitivity and the specificity were 71.4% and 85.1%,respectively,which were better than those of either the ISSAC questionnaires or bronchial provocation test.Conclusion The combination of the free running asthma screening test and total serum allergen IgE level may be an effective diagnostic tool for children's asthma.

  3. Level of anxiety versus self-care in the preoperative and postoperative periods of total laryngectomy patients 1

    Almonacid, Clara Inés Flórez; Ramos, Alfredo Jurado; Rodríguez-Borrego, María-Aurora

    2016-01-01

    Abstract Objective: estimate the prevalence of anxiety in laryngectomy patients in the pre and postoperative periods and its relation with the self-care level. Method: observational research of 40 patients with stage IV laryngeal cancer. Three observations took place: in the preoperative phase, at seven and at 14 days after the surgery; between June 2010 and December 2012. Two self-care levels were defined: self-sufficient and needing help for activities of daily living and treatment-related activities. To assess the anxiety levels, Zigmond's hospital anxiety scale (1983) was used. Results: in the preoperative and postoperative phases, the patients presented high levels of anxiety. Concerning self-care, on average, self-sufficient patients presented lower levels of anxiety than patients who needed help to accomplish activities of daily living and activities deriving from the surgery, without significant differences. Conclusion: anxiety is present at all times in laryngectomy patients and the reduction of the self-care deficit seems to decrease it, without putting a permanent end to it. PMID:27305181

  4. Total joint replacement: A multiple risk factor analysis of physical activity level 1-2 years postoperatively.

    Paxton, Elizabeth W; Torres, Andy; Love, Rebecca M; Barber, Thomas C; Sheth, Dhiren S; Inacio, Maria C S

    2016-07-01

    Background and purpose - The effect of total joint arthroplasty (TJA) on physical activity is not fully understood. We investigated the change in physical activity after TJA and patient factors associated with change. Patients and methods - Using a total joint replacement registry, primary total hip arthroplasty (THA) patients (n = 5,678) and knee arthroplasty (TKA) patients (n = 11,084) between January 1, 2010 and December 31, 2012 were identified. Median age at THA was 68 and median age at TKA was 67. Change in self-reported physical activity (minutes per week) from before TJA (within 1 year of surgery) to after TJA (1-2 years) was the outcome of interest. Patient demographics and comorbidities were evaluated as risk factors. Multiple linear regression was used. Results - Median physical activity before surgery was 50 min/week (IQR: 0-140) for THA patients and 58 (IQR: 3-143) for TKA patients. Median physical activity after surgery was 150 min/week (IQR: 60-280) for both THA patients and TKA patients. Following TJA, 50% of patients met CDC/WHO physical activity guideline criteria. Higher body mass index was associated with lower change in physical activity (THA: -7.1 min/week; TKA: -5.9 min/week). Females had lower change than males (THA: -11 min/week; TKA: -9.1 min/week). In TKA patients, renal failure was associated with lower change (-17 min/week), as were neurological disorders (-30 min/week). Interpretation - Self-reported minutes of physical activity increased from before to after TJA, but 50% of TJA patients did not meet recommended physical activity guideline criteria. Higher body mass index, female sex, and specific comorbidities were found to be associated with low change in physical activity. Patient education on the benefits of physical activity should concentrate on these subgroups of patients. PMID:27299567

  5. Pollution situation and analysis of drinking water source coliform in Pingdingshan%平顶山市饮用水源地总大肠菌群(粪大肠菌群)污染现状及分析

    李世景

    2012-01-01

    In this study,total coliform bacteria was taken as participating factor;fecal coliform as a participating factor for surface water,and multiple tube fermentation method was adopted to monitor and analyze sources of drinking water so as to investigate the pollution status of groundwater and thus to propose feasibility advice and countermeasures.The results showed that the annual total coliform parameters were not super GB/T14848-93 groundwater quality standards for Class III standard limits(MPN≤ 3.0/L) in 2010 and 2011;the average annual concentration of fecal coliform indicators were not super-GB3838-2002 surface water quality standards for class III standard limits(MPN 10,000/L),the water quality reached the national health and safety standards.%地下水以总大肠菌群作为参评因子,地表水以粪大肠菌群作为参评因子,采用多管发酵法,对平顶山市饮用水源地水质进行监测与分析,了解污染现状,提出可行性意见及防治对策。结果表明:2010年、2011年度总大肠菌群指标均未超GB/T14848-93《地下水质量标准》Ⅲ类标准限值(MPN≤3.0个/L),粪大肠菌群指标的年均值均未超GB3838-2002《地表水质量标准》Ⅲ类标准限值(MPN〈10 000个/L),其水质达到国家卫生安全标准。

  6. Total testosterone levels are often more than three times elevated in patients with androgen-secreting tumours

    Glintborg, Dorte; Lambaa Altinok, Magda; Petersen, Kresten Rubeck;

    2015-01-01

    . Gold standard techniques were used to measure testosterone levels. All tumours originated from the ovaries. Based on the present cases and the existing literature, we suggest that androgen-producing tumours should be suspected in patients with rapid progression of hyperandrogen symptoms, particularly...

  7. Total mercury concentrations in an industrialized catchment, the Thur River basin (north-eastern France): geochemical background level and contamination factors

    Rémy, Sandrine; Prudent, Pascale; Hissler, Christophe; Probst, Jean-Luc; Krempp, Gérard

    2003-01-01

    River bottom sediments and soils were collected from the industrialized Thur River basin (north-eastern France) to assess mercury contamination. The regional geochemical background level of total mercury was evaluated to calculate mercury contamination factors (Fc) in soils and river bottom sediments. Our estimate of the mean background mercury levels in river sediments and soils, not affected by human activities, was 232 ng g1 (range: 27–406 ng g1). Sediments contaminated by the effluent fro...

  8. Evaluation of the level of skill required of operators of a computer-assisted radiologic total lung capacity measurement system

    This research was conducted to obtain information regarding the feasibility of using non-medical personnel to obtain measurements of radiologic total lung capacity (TLC). Operators from each of four groups (general undergraduates, nursing students, medical students, radiologists) differing in the amount of medical training and/or experience reading x-rays, performed each of two tasks. The first task was the measurement of radiologic TLC for a set of twenty x-rays. The second task consisted of tracing the outline of the anatomical structures that must be identified in the execution of the radiologic TLC measurement task. Data from the radiologic TLC measurement task were used to identify possible group differences in the reliability and validity of the measures. The reliability analyses were performed within the framework of Generalizability Theory. While the results are not conclusive, due to small sizes, the analyses suggest that group differences in reliability of the measures, if they exist, are small

  9. Total system performance predictions (TSPA-1995) for the potential high-level waste repository at Yucca Mountain

    The management and operating contractor for the potential high-level nuclear waste repository at Yucca Mountain, Nevada, has been recently completed a new performance assessment of the ability of the repository to isolate and contain nuclear waste for long time periods (up to 1,000,000 years). Sensitivity analyses determine the most important physical parameters and processes, using the most current information and models

  10. Tratamento de esgotos sanitários em sistemas reatores UASB/wetlands construídas de fluxo horizontal: eficiência e estabilidade de remoção de matéria orgânica, sólidos, nutrientes e coliformes Domestic wastewater treatment in UASB-horizontal flow constructed wetlands systems: organic matter, solids, nutrients and coliforms removal

    Maria Lúcia Calijuri

    2009-09-01

    Full Text Available Este trabalho apresentou os resultados de um estudo realizado durante 19 meses sobre o comportamento de wetlands construídas na remoção de matéria orgânica, sólidos, nutrientes e coliformes, em unidades em escala piloto de fluxo horizontal, subsuperficial e superficial, com tempo de detenção hidráulica entre 1,3 a 5,3 dias, operando como pós-tratamento de efluentes de reatores UASB (esgotos sanitários. A remoção de matéria orgânica e de sólidos mostrou-se elevada e estável, com eficiências médias de 70, 80 e 60% para SST, DBO5 e DQO, respectivamente. A remoção de nutrientes, após início promissor, mostrou-se instável e aparentemente influenciada pela temperatura. O sistema de tratamento revelou elevado potencial de remoção de coliformes, embora com variações relativamente amplas ao longo do período de operação: ≈ 2 log10 de remoção de coliformes totais e 2-4 log10 de remoção de Escherichia coli.This work presented the results of a 19-month study on the performance of constructed wetlands in terms of organic matter, solids, nutrients and coliforms removal in pilot scale unities with horizontal, subsurface and surface flow, with hydraulic retention time from 1.3 to 5.3 days, as post-treatment of UASB effluents (domestic wastewater. Organic matter and solids were effectively and consistently removed, with average values of 70, 80 and 60% for TSS, BOD5 and COD, respectively. Nutrients removal, after a promising start up, became unstable and apparently influenced by temperature. The treatment system has also shown high potential to remove coliforms, although with relatively wide variations over the study period: ≈ 2 log10 reduction of total coliforms and 2-4 log10 reduction of Escherichia coli.

  11. Survey of total and inorganic arsenic content in blue mussels (Mytilus edulis L.) from Norwegian fiords: Revelation of unusual high levels of inorganic arsenic

    Sloth, Jens Jørgen; Julshamn, Kåre

    2008-01-01

    The present study reports the findings of unusual high levels of inorganic arsenic in samples of blue mussels (Mytilus edulis L.). A total of 175 pooled samples of blue mussels from various locations along the Norwegian coastline were analyzed for their content of total arsenic and inorganic...... arsenic. Total arsenic was determined using inductively coupled plasma mass spectrometry (ICPMS) following microwave-assisted acidic digestion of the samples. Inorganic arsenic was determined using an anion-exchange HPLC-ICPMS method following microwave-assisted alkaline solubilization of the samples....... For the majority of the samples (78%) the concentration of total arsenic was below 3 mg kg(-1) wet weight (ww) and inorganic arsenic constituted...

  12. Removal of Escherichia coli and Faecal Coliforms from Surface Water and Groundwater by Household Water Treatment Devices/Systems: A Sustainable Solution for Improving Water Quality in Rural Communities of the Southern African Development Community Region

    Jocelyne K. Mwabi

    2012-01-01

    Full Text Available There is significant evidence that household water treatment devices/systems (HWTS are capable of dramatically improving microbially contaminated water quality. The purpose of this study was to examine five filters [(biosand filter-standard (BSF-S; biosand filter-zeolite (BSF-Z; bucket filter (BF; ceramic candle filter (CCF; and silver-impregnated porous pot (SIPP] and evaluate their ability to improve the quality of drinking water at the household level. These HWTS were manufactured in the workshop of the Tshwane University of Technology and evaluated for efficiency to remove turbidity, faecal coliforms and Escherichia coli from multiple water source samples, using standard methods. The flow rates ranged from 0.05 L/h to 2.49 L/h for SIPP, 1 L/h to 4 L/h for CCF, 0.81 L/h to 6.84 L/h for BSF-S, 1.74 L/h to 19.2 L/h and 106.5 L/h to 160.5 L/h for BF The turbidity of the raw water samples ranged between 2.17 and 40.4 NTU. The average turbidity obtained after filtration ranged from 0.6 to 8 NTU (BSF-S, 1 to 4 NTU (BSF-Z, 2 to 11 NTU (BF, and from 0.6 to 7 NTU (CCF and 0.7 to 1 NTU for SIPP. The BSF-S, BSF-Z and CCF removed 2 to 4 log10 (99% to 100% of coliform bacteria, while the BF removed 1 to 3 log (90% to 99.9% of these bacteria. The performance of the SIPP in removing turbidity and indicator bacteria (>5 log10, 100% was significantly higher compared to that of the other HWTS (p < 0.05. The findings of this study indicate that the SIPP can be an effective and sustainable HWTS for the Southern African Development Community (SADC rural communities, as it removed the total concentration of bacteria from test water, can be manufactured using locally available materials, and is easy to operate and to maintain.

  13. Simultaneous multi-level analysis of the total and fission cross-sections of 239Pu up to 160 eV

    In order to describe cross-sections a multi-level scheme based on S-matrix theory was used taking the Doppler effect and the resolution into account. A multi-level analysis of the total cross-section and fission cross-section of 239Pu was made by the least-squares method in the energy region up to 160 eV. The experimental cross-section data used have a good resolution. The multi-level parameters can be used to represent all the features of the detailed energy structure of experimental cross-sections where the regions of interference minima are of the greatest interest. (author)

  14. Use of {sup 60}Co gamma radiation in increased levels of total polyphenol extracts of bark of Schinus terebinthifolius Raddi

    Santos, Gustavo H.F.; Silva, Edvane B.; Silva, Hianna A.M.F.; Amorin, Elba L.C.; Peixoto, Tadeu J.S.; Yara, Ricardo; Lima, Claudia S.A., E-mail: santosghf@hotmail.com, E-mail: edvborges@yahoo.com, E-mail: amdemelo@hotmail.com, E-mail: claudia.salima@gmail.com, E-mail: ricardo.yara@gmail.com, E-mail: tadeu1903@yahoo.com.br, E-mail: elba@ufpe.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2013-07-01

    Schinus terebinthifolius Raddi (Anacardiaceae) is well known as sources of phenolic compounds. Known as mastic pepper, red pepper tree is a plant native to midsize coast of Brazil. Some of its structures have proven antibacterial, anti-inflammatory, antifungal and healing. The aim of this study was to evaluate the difference in the phenol contents of crude extracts that were measured after irradiating the barks of S. terebinthifolius using gamma radiation from {sup 60}Co. The crude extract were divided into a control group and eight experimental groups, which were separated based on the doses of gamma radiation to which they were exposed: 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 20.0 and 50.0 kGy (Assays were performed in triplicate). The results allow observe that gamma radiation promoted in extracts of bark of S. terebinthifolius, many percents increase (p> 0.05) of total polyphenol content between 2.5 kGy (41.93%) and 50.0 kGy (44.52%) compared to 0 kGy (30.07%), with the same gradual to 10.0 kGy, and reaching peak maximum at 10.0 kGy (68.44%). However, the study puts the process of gamma radiation from {sup 60}Co as an alternative significant increase in the percentage of some natural substances of plant material, and subsequently contribute to the augmentation of various therapeutic applications to which they are assigned. (author)

  15. Use of 60Co gamma radiation in increased levels of total polyphenol extracts of bark of Schinus terebinthifolius Raddi

    Schinus terebinthifolius Raddi (Anacardiaceae) is well known as sources of phenolic compounds. Known as mastic pepper, red pepper tree is a plant native to midsize coast of Brazil. Some of its structures have proven antibacterial, anti-inflammatory, antifungal and healing. The aim of this study was to evaluate the difference in the phenol contents of crude extracts that were measured after irradiating the barks of S. terebinthifolius using gamma radiation from 60Co. The crude extract were divided into a control group and eight experimental groups, which were separated based on the doses of gamma radiation to which they were exposed: 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 20.0 and 50.0 kGy (Assays were performed in triplicate). The results allow observe that gamma radiation promoted in extracts of bark of S. terebinthifolius, many percents increase (p> 0.05) of total polyphenol content between 2.5 kGy (41.93%) and 50.0 kGy (44.52%) compared to 0 kGy (30.07%), with the same gradual to 10.0 kGy, and reaching peak maximum at 10.0 kGy (68.44%). However, the study puts the process of gamma radiation from 60Co as an alternative significant increase in the percentage of some natural substances of plant material, and subsequently contribute to the augmentation of various therapeutic applications to which they are assigned. (author)

  16. Mutation in APOA1 predicts increased risk of ischaemic heart disease and total mortality without low HDL cholesterol levels

    Haase, C L; Frikke-Schmidt, R; Nordestgaard, B G;

    2011-01-01

    levels. Mutations in apolipoprotein (apo) A-I, the major protein constituent of HDL, might be associated with low HDL cholesterol and predispose to IHD and early death. DESIGN: We resequenced APOA1 in 190 individuals and examined the effect of mutations on HDL cholesterol, risk of IHD, myocardial...... infarction (MI) and mortality in 10 440 individuals in the prospective Copenhagen City Heart Study followed for 31 years. Results were validated in an independent case-control study (n = 16 035). Additionally, we determined plasma ratios of mutant to wildtype (WT) apoA-I in human heterozygotes and functional.......3-4.8), respectively, in heterozygotes compared with noncarriers. Mean reduction in survival time in heterozygotes was 10 years (P < 0.0001). Results for IHD and MI were confirmed in the case-control study. Furthermore, the ratio of mutant S164 to WT A164 apoA-I in plasma of heterozygotes was reduced. In addition, A...

  17. Preliminary total-system analysis of a potential high-level nuclear waste repository at Yucca Mountain

    The placement of high-level radioactive wastes in minded repositories deep underground is considered a disposal method that would effectively isolate these wastes from the environment for long periods of time. This report describes modeling performed at PNL for Yucca Mountain between May and November 1991 addressing the performance of the entire repository system related to regulatory criteria established by the EPA in 40 CFR Part 191. The geologic stratigraphy and material properties used in this study were chosen in cooperation with performance assessment modelers at Sandia National Laboratories (SNL). Sandia modeled a similar problem using different computer codes and a different modeling philosophy. Pacific Northwest Laboratory performed a few model runs with very complex models, and SNL performed many runs with much simpler (abstracted) models

  18. Preliminary total-system analysis of a potential high-level nuclear waste repository at Yucca Mountain

    Eslinger, P.W.; Doremus, L.A.; Engel, D.W.; Miley, T.B.; Murphy, M.T.; Nichols, W.E.; White, M.D. [Pacific Northwest Lab., Richland, WA (United States); Langford, D.W.; Ouderkirk, S.J. [Westinghouse Hanford Co., Richland, WA (United States)

    1993-01-01

    The placement of high-level radioactive wastes in mined repositories deep underground is considered a disposal method that would effectively isolate these wastes from the environment for long periods of time. This report describes modeling performed at PNL for Yucca Mountain between May and November 1991 addressing the performance of the entire repository system related to regulatory criteria established by the EPA in 40 CFR Part 191. The geologic stratigraphy and material properties used in this study were chosen in cooperation with performance assessment modelers at Sandia National Laboratories (SNL). Sandia modeled a similar problem using different computer codes and a different modeling philosophy. Pacific Northwest Laboratory performed a few model runs with very complex models, and SNL performed many runs with much simpler (abstracted) models.

  19. Heat-to-heat variations of total strain ;to 5 ]3<: at discrete stress levels in types 316 and 304 stainless steel from 24 to 316

    Tensile tests were conducted on eight heats of randomly selected, mill-annealed type 316 stainless steel and seven heats of mill-annealed type 304 stainless steel at 24, 93, 204, and 3160C to establish the effects of heat-to-heat variations on total strain (to 5 percent) at discrete stress levels. Factors of difference were established between maximum and average strain (corresponding to minimum and nominal yield strength material respectively) for regulatory analysis applications. Limited tests were conducted to show the effects of product form and material condition on total strain. 13 tables, 25 fig

  20. Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits

    Jacopo Gervasoni

    2013-01-01

    Full Text Available Objective. 25-hydroxyvitamin D2/D3 (25-OHD2/D3 determination is a reliable biomarker for vitamin D status. Liquid chromatography-tandem mass spectrometry was recently proposed as a reference method for vitamin D status evaluation. The aim of this work is to compare two commercial kits (Chromsystems and PerkinElmer for 25-OHD2/D3 determination by our entry level LC-MS/MS. Design and Methods. Chromsystems kit adds an online trap column to an HPLC column and provides atmospheric pressure chemical ionization, isotopically labeled internal standard, and 4 calibrator points. PerkinElmer kit uses a solvent extraction and protein precipitation method. This kit can be used with or without derivatization with, respectively, electrospray and atmospheric pressure chemical ionization. For each analyte, there are isotopically labeled internal standards and 7 deuterated calibrator points. Results. Performance characteristics are acceptable for both methods. Mean bias between methods calculated on 70 samples was 1.9 ng/mL. Linear regression analysis gave an of 0.94. 25-OHD2 is detectable only with PerkinElmer kit in derivatized assay option. Conclusion. Both methods are suitable for routine. Chromsystems kit minimizes manual sample preparation, requiring only protein precipitation, but, with our system, 25-OHD2 is not detectable. PerkinElmer kit without derivatization does not guarantee acceptable performance with our LC-MS/MS system, as sample is not purified online. Derivatization provides sufficient sensitivity for 25-OHD2 detection.

  1. Significance analysis of the leachate level in a solid waste landfill in a coastal zone using total water balance and slope stability alternatives

    Koo, Ja-Kong; Do, Nam-Young [Korea Advanced Institute of Science & Technology, Taejon (Korea, Republic of)

    1996-12-31

    The K site near Seoul began landfilling in 1992. The landfilled wastes include municipal solid waste (66.4%), construction residues (20.4%), water and wastewater sludges (trace levels), and hazardous waste (trace levels). The water content of the municipal solid waste is very high (47.3%); as a result, the leachate level (average E.L.) of the landfill, the design value of which is 7.0 m, was measured at 10.3 m in January 1995 and is increasing. The increase of leachate level in the landfill site causes a problem with slope stability. The leachate level at each disposal stage divided by the intermediate cover layer was calculated with the HELP (Hydrologic Evaluation of Landfill Performance) model and calibrated with the data measured from February 1993 to June 1995. Also, the hydraulic conductivities of the waste layer and the intermediate cover layer in each stage were calibrated continuously with HELP model analysis. To verify these results, the total water balance in the landfill site was calculated using the infiltration rate calculated from HELP modeling. The leachate level was E.L. 10.0 m, which was close to the measured leachate level. To estimate the change of the leachate level in the future, the total water balances with different leachate discharge rates of 3,000, 3,500, and 5,000 m{sup 3}/day were analyzed. When the leachate discharge rate was 5,000 ton/day and the initial water content was decreased below 25%, the average leachate level was 10.8 m. This result satisfies the safety factor requirements (=1.3) for landfill slope stability. 4 refs., 8 figs., 1 tab.

  2. Effect of carnosine supplementation on apoptosis and irisin, total oxidant and antioxidants levels in the serum, liver and lung tissues in rats exposed to formaldehyde inhalation.

    Aydin, Suna; Ogeturk, Murat; Kuloglu, Tuncay; Kavakli, Ahmet; Aydin, Suleyman

    2015-02-01

    The main objective of the study has been to show whether carnosine has positive effects on liver and lung tissues of rats exposed to a range of formaldehyde concentrations, and to explore how irisin expression and antioxidant capacity are altered in these tissues by carnosine supplementation. Sprague-Dawley type male rats were divided into 8 groups with 6 animals in each: (I) Control; no chemical supplementation); (II) sham (100mg/kg/day carnosine); (III) low dose formaldehyde (LDFA) for 5 days/week; (IV) LDFA for 5 days/week and carnosine); (V) moderate dose formaldehyde (MDFA) for 5 days/week); (VI) MDFA for 5 days/week and carnosine; (VII) high dose formaldehyde (HDFA) for 5 days/week; (VIII) and HDFA for 5 days/week and carnosine. Sham and control groups were exposed to normal air. Irisin levels of the serum, liver and lung tissue supernatants were analyzed by ELISA, while the REL method was used to determine total oxidant/antioxidant capacity. Irisin production by the tissues was detected immunohistochemically. Increasing doses of FA decreased serum/tissue irisin and total antioxidant levels relative to the controls, as also to increases in TUNEL expressions, total oxidant level, oxidant and apoptosis index. Irisin expression was detected in hepatocyte and sinusoidal cells of the liver and parenchymal cells of the lung. In conclusion, while FA exposure reduces irisin and total oxidant in the serum, liver and lung tissues in a dose-dependent manner and increases the total antioxidant capacity, carnosine supplementation reduces the oxidative stress and restores the histopathological and biochemical signs. PMID:25541044

  3. The Analysis of Different Culture Time Affect Test Results of Coliform Bacteria Primary Fermentation%不同培养时间对大肠菌群初发酵检测结果的影响分析

    宋昌彦; 盛满钰

    2012-01-01

    为了了解不同培养时间对大肠菌群初发酵检测结果的影响,为大肠菌群检测方法、卫生标准的修订及食源性疾病的预防和控制提供科学依据。在同一品牌快餐店随机采集具有代表性的6大类342件样品进行大肠菌群检测,得到有效样品数为254件。依照国家标准进行检验和结果评价,并将初发酵培养时间为(24±2)h和(48±2)h的检测结果进行比较。结果表明,样品经(48±2)h初发酵培养的大肠菌群检出率及LST肉汤阳性率均高于(24±2)h,其差异均具有统计学意义Co〈o.01)。因此,大肠菌群迟缓发酵现象普遍存在。适当延长初发酵培养时间将有利于提高大肠菌群的检出率,从而更真实的反映食品的卫生质量状况。2010年6月正式实施的GB4789.3—2010,与2003版相比其结果表达发生了变化,而相应的很多产品卫生标准仍未同时更新。%In order to understand what effect on the different culture period of coliform bacteria cause on the test result of coliforms primary fermentation, provide the scientific proof for coliform bacteria detection methods, and revise health standard and prevention and control of food-borne disease, a total of 342 representative samples of six main categories were randomly collected in various chain stores of the same brand fast-food restaurant for coliform bacteria testing. Finally, 254 effective samples were got. In accordance with national standards for testing and evaluation of results, and to compare test results between (24~:2) h and (48~2) h on the coliforms primary fermentation time, according to results of the comparison view, the sample after (48h:k2) h primary fermentation coliform .detection rate and positive rate of LST broth were higher than the (24+2) h, the differences are statistically significant (p〈0.01). Therefore, coliform slow fermentation is widespread and ubiquity. Extend the time of primary

  4. Total blood mercury levels and depression among adults in the United States: National Health and Nutrition Examination Survey 2005-2008.

    Tsz Hin H Ng

    Full Text Available BACKGROUND: Mercury is a neurotoxicant linked with psychiatric symptoms at high levels of exposure. However, it is unclear whether an association is present at the low exposure levels in the US adult population. MATERIALS AND METHODS: Cross-sectional associations of total blood mercury and depression were assessed in 6,911 adults age ≥20 in the National Health and Nutrition Examination Survey (NHANES, 2005-2008. The Patient Health Questionnaire-9 was used to assess depression (high likelihood of a depressive spectrum disorder diagnosis; score 5-27. RESULTS: Unadjusted survey weighted logistic regression suggested that higher total blood mercury was associated with lower odds of depression (Odds Ratio  = 0.49, 95% Confidence Interval: 0.36-0.65, comparing the highest and lowest mercury quintiles. This association largely disappeared after adjustment for sociodemographic variables (income-poverty ratio, education, marital status. However, in age-stratified analyses, this inverse relationship remained in older adults (age ≥40 even after adjustment for sociodemographic variables. Simulation analyses adjusting for expected confounding effects of fish intake suggested that the inverse relationship among older adults may be plausibly attributed to residual confounding (Odds Ratio  = 0.75, 95% Confidence Interval: 0.50-1.12, comparing the highest and lowest mercury quintiles. CONCLUSIONS: Higher total blood mercury was not associated with increased odds of depression. The lower odds of depression in older adults with higher total blood mercury may be due to residual confounding.

  5. Can Serum Levels of Alkaline Phosphatase and Phosphate Predict Cardiovascular Diseases and Total Mortality in Individuals with Preserved Renal Function? A Systemic Review and Meta-Analysis

    Jing-Wei Li; Cui Xu; Ye Fan; Yong Wang; Ying-Bin Xiao

    2014-01-01

    Background It is demonstrated that elevated serum levels of alkaline phosphatase (ALP) and phosphate indicate a higher risks of cardiovascular disease (CVD) and total mortality in population with chronic kidney disease (CKD), but it remains unclear whether this association exists in people with normal or preserved renal function. Method Clinical trials were searched from Embase and PubMed from inception to 2013 December using the keywords “ALP”, “phosphate”, “CVD”, “mortality” and so on, and ...

  6. Effect of plant extract of Tribulus terrestris and probiotics on the reproductive performance, total cholesterol and testosterone hormone levels of rams

    Sharawy, S. M.; Saleh, N. H.; Attalah, S. A.; Absy, G. M.; Doaa, H. K.

    2015-01-01

    The objective of this experiment was to compare between the effects of tribulus terrestris extract and probiotics on scrotal circumference, testicular volume, reaction time, semen characteristics, serum total cholesterol and testosterone hormone levels of rams. The study was carried out from February to July 2013 on eight sexually mature Rahmani rams. The eight rams were used as control group for one month before treatment (weekly assessment for all the parameters). Rams were divided into two...

  7. The Effect of Chromium Added into Basal Diet on Serum Total Protein, Urea, Triglyceride, Cholesterol and Serum and Tissue Chromium, Zinc, Copper Levels in Rabbits

    *, Kâzim ŞAHİN; *, Talat GÜLER; +, N. ŞAHİN; *, O. N. ERTAS; +, N. ERKAL

    1999-01-01

    This study was conducted to determine the effect of supplemantal dietary chromium on serum total protein, urea, triglycerides, cholesterol, and serum and tissue chromium, zinc, and copper contents of pregnant rabbits, their offspring and their young rabbits. Treatment groups consisted of chromium level as follows: Control Group no supplementation chromium into basal diet, Treatment I (200 ppb Group) contained 200 ppb of supplemental chromium into basal diet, and Treatment II (400 ppb Group...

  8. Effect of Short-Term Maximal Exercise on Biochemical Markers of Muscle Damage, Total Antioxidant Status, and Homocysteine Levels in Football Players

    Hammouda, Omar; Chtourou, Hamdi; Chaouachi, Anis; Chahed, Henda; Ferchichi, Salyma; Kallel, Choumous; Chamari, Karim; Souissi, Nizar

    2012-01-01

    Purpose Prolonged physical exercise results in transient elevations of biochemical markers of muscular damage. This study examined the effect of short-term maximal exercise on these markers, homocysteine levels (Hcy), and total antioxidant status (TAS) in trained subjects. Methods Eighteen male football players participated in this study. Blood samples were collected 5-min before and 3-min after a 30-s Wingate test. Results The results indicated that plasma biochemical markers of muscle injur...

  9. Total petroleum content and polycyclic aromatic hydrocarbon levels in soil and groundwater at the site of a fuel service station in Accra

    Analysis of Total Petroleum Content (TPC) and Polycyclic Aromatic Hydrocarbon (PAH) in soil and groundwater at the site of a fuel service station indicated leakage of petroleum products from the underground storage tanks buried 3.6km below the surface. The leakage resulted in contamination of areas within a 60 m radius. In the TPC profiles, distinct peak corresponding to diesel oil occurred between 5 and 17 mins; while a hump characteristic of mineral oil occurred between 17 and 25 mins. In the PAH profiles, 18 compounds were identified and quantified by means of gas chromatography. The TPC levels ranged between 0.918 - 21.9 μg/L for ground water and 44.1 - 894 mg/kg for soil. The levels of PAHs were low and HM-PAHs were not detected. The total PAH levels varied between 20.93 - 51.85 μg/L for groundwater and 2.07 - 6.5 ng/kg ww for soil. The levels of LM-PAHs were above the International standard of 200 ng/L, while BaP levels were 710 ng/L (indicative of carcinogenic effects). Naphthalene was found to be abundant in the groundwater. (au)

  10. Effect of varying levels of formaldehyde treatment of mustard oil cake on rumen fermentation, digestibility in wheat straw based total mixed diets in vitro

    Mahima

    2015-04-01

    Full Text Available Aim: The aim of the current study was to protect the protein in mustard cake by different levels of formaldehyde treatment with a view to optimize the level of formaldehyde. Materials and Methods: Different levels of formaldehyde treatment (0, 1, 1.5 and 2% of crude protein containing concentrate and roughages diet in 40:60 ratio were tested for their effect on nutrients digestibility, in vitro ammonia release, in vitro gas production and change in protein fractions. Non-significant (p≤0.05 effect on pH, microbial biomass, partitioning factor, total gas production (TGP, TGP per g dry matter and TGP per g digestible dry matter (ml/g was observed in almost all the treatments. Results: Total volatile fatty acids at 2% formaldehyde treatment level of mustard cake was lower (p<0.05 as compared to other groups, while in vitro dry matter digestibility and in vitro organic matter digestibility were reported to be low in 1% formaldehyde treated group. Conclusion: On a holistic view, it could be considered that formaldehyde treatment at 1.5% level was optimal for protection of mustard oil cake protein.

  11. Spatial variability in levels of benzene, formaldehyde, and total benzene, toluene, ethylbenzene and xylenes in New York City: a land-use regression study

    Kheirbek Iyad

    2012-07-01

    Full Text Available Abstract Background Hazardous air pollutant exposures are common in urban areas contributing to increased risk of cancer and other adverse health outcomes. While recent analyses indicate that New York City residents experience significantly higher cancer risks attributable to hazardous air pollutant exposures than the United States as a whole, limited data exist to assess intra-urban variability in air toxics exposures. Methods To assess intra-urban spatial variability in exposures to common hazardous air pollutants, street-level air sampling for volatile organic compounds and aldehydes was conducted at 70 sites throughout New York City during the spring of 2011. Land-use regression models were developed using a subset of 59 sites and validated against the remaining 11 sites to describe the relationship between concentrations of benzene, total BTEX (benzene, toluene, ethylbenzene, xylenes and formaldehyde to indicators of local sources, adjusting for temporal variation. Results Total BTEX levels exhibited the most spatial variability, followed by benzene and formaldehyde (coefficient of variation of temporally adjusted measurements of 0.57, 0.35, 0.22, respectively. Total roadway length within 100 m, traffic signal density within 400 m of monitoring sites, and an indicator of temporal variation explained 65% of the total variability in benzene while 70% of the total variability in BTEX was accounted for by traffic signal density within 450 m, density of permitted solvent-use industries within 500 m, and an indicator of temporal variation. Measures of temporal variation, traffic signal density within 400 m, road length within 100 m, and interior building area within 100 m (indicator of heating fuel combustion predicted 83% of the total variability of formaldehyde. The models built with the modeling subset were found to predict concentrations well, predicting 62% to 68% of monitored values at validation sites. Conclusions Traffic and

  12. Thyroid stimulating hormone, independent of thyroid hormone, can elevate the serum total cholesterol level in patients with coronary heart disease: a cross-sectional design

    Xu Chao

    2012-05-01

    Full Text Available Abstract Background The relationship between TSH and the lipid profile is contradictory because few studies have excluded the potential influence of the thyroid hormones (TH. The aim of the present study was to evaluate the relationship between serum TSH levels and the lipid profile independent of TH. Methods 1302 CHD patients diagnosed by coronary angiography were retrospectively studied. The prevalence and distribution of thyroid dysfunction were analyzed first. To assess the impact of TSH on serum lipids, Pearson’s correlation analysis was performed after adjustments for classic factors and TH. To calculate the extent of the effect of TSH on the serum cholesterol level, the partial least squares method and additional statistical methods were used. Results After the exclusions, a total of 568 patients (270 males and 298 females with a mean age of 63.56 ± 11.376 years were selected. The prevalence of thyroid dysfunction among the patients was 18.66%, and the prevalence of hypothyroidism (15.32% was higher than that of hyperthyroidism (3.34%. Even after adjusting for confounding factors, such as sex, age, smoking status, fasting plasma glucose levels and TH, a significant positive impact of TSH on the serum total cholesterol (TC level was revealed (r = 0.095, p = 0.036. Each 1 mIU/L increase in the TSH level might be linked to a 0.015580712 mmol/L elevation of the serum TC value. Conclusions TSH can increase the TC level in CHD patients independent of TH. The present study suggests a potential physiological role of TSH and the importance of maintaining an appropriate TSH level in CHD patients.

  13. Serum Total Tryptase Level Confirms Itself as a More Reliable Marker of Mast Cells Burden in Mast Cell Leukaemia (Aleukaemic Variant

    P. Savini

    2015-01-01

    Full Text Available Mast cell leukemia (MCL is a very rare form of systemic mastocytosis (SM with a short median survival of 6 months. We describe a case of a 65-year-old woman with aleukaemic variant of MCL with a very high serum total tryptase level of 2255 μg/L at diagnosis, which occurred following an episode of hypotensive shock. She fulfilled the diagnostic criteria of SM, with a bone marrow smear infiltration of 50–60% of atypical mast cells (MCs. She tested negative for the KIT D816V mutation, without any sign of organ damage (no B- or C-findings and only few mediator-related symptoms. She was treated with antihistamine alone and then with imatinib for the appearance of anemia. She maintained stable tryptase level and a very indolent clinical course for twenty-two months; then, she suddenly progressed to acute MCL with a serum tryptase level up to 12960 μg/L. The patient died due to haemorrhagic diathesis twenty-four months after diagnosis. This clinical case maybe represents an example of the chronic form of mast cell leukemia, described as unpredictable disease, in which the serum total tryptase level has confirmed itself as a reliable marker of mast cells burden regardless of the presence of other signs or symptoms.

  14. Total mercury and methylmercury levels in pregnant women, nursing women and preschool children resident in fishing villages in the eighth region of Chile

    The main aim of this project is to perform a descriptive study about the levels of total mercury (Hg-T) and of methylmercury (Me-Hg) in scalp hair specimens of a selected human population of high risk in the Eighth Region of Chile, the group studied included pregnant women (PW), nursing women (NW) and preschool children residing in fishing villages distributed within the coastal zone of this region, the diets of the test group included fish and shellfish as main food components. The degree of Hg contamination of this population was compared to a control population (''core programme''). The methylmercury-to-total mercury ratio (Me-Hg/Hg-T) levels in scalp hair enabled interpretation of the results with respect to the degree of contamination by Hg, and the dietary habits of the sample donors of each fishing village under study. Furthermore, Se levels in scalp hair of the populations with relatively high Hg content were to be investigated for possible correlation with Me-Hg levels (''supplementary programme''). 5 refs, 2 figs, 9 tabs

  15. Modeling Fate and Transport of Fecal Coliform Bacteria Using SWAT 2005 (Case Study: Jajrood River Watershed, Iran)

    Maghrebi, M.; Tajrishy, M.

    2010-12-01

    Jajrood River watershed is one of the main drinking water resources of the capital city of Tehran, Iran. In addition it has been available as many recreational usages especially in the warm months. As a result of being located near one of the crowded cities of the world, a variety of microbial pollutions is commonly perceived in the Jajrood River. Among them, there are strong concerns about fecal coliform bacteria concentration. This article aimed to model fate and transport of fecal coliform bacteria in Jajrood River watershed using Soil and Water Assessment Tool (SWAT) model version 2005. Potential pollutant sources in the study area were detected and quantified for modeling purposes. In spite of being lack of knowledge about bacteria die-off rate in small river bodies, as well as in other watershed-based forms, fecal coliform bacteria die-off rates were estimated using both laboratory and field data investigations with some simplifications. The SWAT model was calibrated over an extended time period (1997-2002) for this watershed. The river flow calibrated using SUFI-2 software and resulted in a very good outputs (R2=0.82, E=0.81). Furthermore SWAT model was validated over January 2003 to September 2005 in the study area and has resulted in good outputs (R2=0.61, E=0.57). This research illustrates SWAT 2005 capability to model fecal coliform bacteria in a populated watershed, and deals with most of watershed microbial pollution sources that are usually observed in developing countries. Fecal coliform concentration simulation results were mostly in the same order in comparison with real data. However, Differences were judged to be related to lack of input data. In this article different aspects of SWAT capabilities for modeling of fecal coliform bacteria concentration will be reviewed and it will present new insights in bacteria modeling procedures especially for mountainous, high populated and small sized watersheds.

  16. Effect of meteorologic conditions on total suspended particulate (TSP) levels and elemental concentration of aerosols in a semi-arid zone (Beer-Sheva, Israel)

    The total suspended particulate (TSP) levels in Beer-Sheva, situated in a semi-arid area with winds bringing in dust from the surrounding deserts, was measured 41 times between June 1977 and May 1978. The TSP levels are correlated with climatic conditions. High TSP levels (890 +- 250 μg m-3) were found during Sharav conditions and sandstorms (hot, dry weather, typical to this region). Low TSP levels (59 +- 28 μg m-3) were found in the winter following rain. Usual TSP levels were found to be 140 +- 20 μg m-3 occurring during normal weather conditions. The elemental composition of the Beer-Sheva aerosols were determined using instrumental neutron activation analysis (INNA) and X-ray Fluorescence (XRF). The concentrations of the following elements Fe, Na, Br, K, Sm, Au, Sb, Ga, La, Yb, Lu, Se, Hg, Cr, Sc, Rb, Co, Ta and Zn were determined by INNA and Ca, Si, S, Ti, Cl, Pb, Fe, V, Ni and Mn by XRF. Their relative abundances were found to depend on the prevailing TSP level. (Auth.)

  17. Effect of heavy metals on the level of vitamin E, total lipid and glycogen reserves in the liver of common carp (Cyprinus carpio L.

    Vinodhini Rajamanickam

    2008-06-01

    Full Text Available The aim of this study is to examine some changes in the biochemical profile of the liver tissue of common carp (Cyprinus carpio L. exposed to a sublethal concentration of heavy metal mixture (cadmium, chromium, nickel and lead. The biochemical profile, specifically glycogen, total lipid and vitamin E content in the liver tissue was examined and compared to that of the control group. The exposed group showed a marked decline in glycogen and vitamin E reserves. Conversely an increase in total lipid in comparison to control was observed. The result reflects the sensitivity of these biochemical parameters to the effects of sublethal levels of combined heavy metals for this the widely consumed freshwater fish.

  18. Investigação teórica do processo de redução de coliformes em leitos cultivados Theoretical investigation of coliform reduction in constructed wetland

    Mariângela Amendola

    2007-12-01

    on the implicit scheme of the finite difference method was implemented which, when ran with MATLAB 6.1, generated values for the pollutant concentration which depend on the mentioned constants. The results obtained with the simulation were compared with the reference values to determine the longitudinal dispersion coefficient value which leads to the least residual value for each kind of support bed. The obtained results lead to the conclusion that the constructed wetland with crushed stone and chopped tires is better than the one with crushed stone alone to reduce fecal and total coliforms.

  19. Unexpected high PCB and total DDT levels in the breeding population of red kite (Milvus milvus) from Doñana National Park, south-western Spain.

    Gómara, Belén; González, María José; Baos, Raquel; Hiraldo, Fernando; Abad, Esteban; Rivera, Josep; Jiménez, Begoña

    2008-01-01

    This study provides information on the current status of contamination by organochlorines (DDTs, PCBs, PCDDs and PCDFs) in the declining red kite (Milvus milvus L.) population breeding in the Doñana National Park (DNP), south-western Spain. Analyses were performed in addled eggs collected between 1999 and 2001. DDE concentrations ranged from 0.1 to 33.5 microg/g ww, representing more than 86% of the total DDTs. Of the samples studied, 50% showed DDE levels above those associated with reproductive impairment in other raptor species. Concentrations of ortho PCBs (average 36.8 microg/g ww+/-37.7) in 50% of the eggs were much higher than levels reported to cause reduced hatching success, embryo mortality, and deformities in birds (>20 microg/g ww). It is remarkable that average ortho PCB and DDE concentrations showed an increase of one order of magnitude compared to previous data for the species during the 80s. Total PCDD/Fs showed levels in the low pg/g range (7.2-42 pg/g ww), having PCDDs and PCDFs similar contributions in most samples. Total mean TEQs were 238 pg/g (ww), being the range 7.02-667 pg/g (ww). Spatial variation within DNP was observed for PCBs, DDTs, as well as for TEQs. Since some eggs exceeded the NOEL (67%) and LOEL (33%) reported for other raptor species, we would expect the red kite to experience detrimental effects to dioxin-like toxicity. Our results suggest that organochlorine contaminants should be regarded as an element of concern in the population under study, in addition to other conservation problems already reported. Further investigations should be undertaken to identify potential sources of these chemicals in DNP, and to find out if organochlorine contamination is present in other predator species in the area, as well as their potential health effects on individuals and/or populations. PMID:17727950

  20. Total mercury and methylmercury levels in pregnant women, nursing women and preschool children resident in fishing villages in the eighth region of Chile

    The main aim of this project is to perform a descriptive study about the levels of total mercury (Hg-T) and of methylmercury (Me-Hg) in human scalp hair specimens of a selected human population of high risk in the Eighth Region of Chile (composed by pregnant women, nurse women and prescholar children residing in fisheries distributed within the coastal zone of this region), whose diet should include fish and shellfish as main food components, and to evaluate the degree of mercury contamination of this population by comparison with a control population. 6 refs, 2 tabs

  1. Unexpected high PCB and total DDT levels in the breeding population of red kite (Milvus milvus) from Doñana National Park, south-western Spain

    Gómara, B.; González, María José; Baos, Raquel; Hiraldo, F.; Abad, Esteban; Rivera, Josep; Jiménez, Begoña

    2008-01-01

    This study provides information on the current status of contamination by organochlorines (DDTs, PCBs, PCDDs and PCDFs) in the declining red kite (Milvus milvus L.) population breeding in the Doñana National Park (DNP), south-western Spain. Analyses were performed in addled eggs collected between 1999 and 2001. DDE concentrations ranged from 0.1 to 33.5 μg/g ww, representing more than 86% of the total DDTs. Of the samples studied, 50% showed DDE levels above those associated with reproductive...

  2. Examining the quasibiennial oscillation of total ozone and ozone concentrations at separate stratospheric levels according to data of TOMS satellite instrumentation

    Bazhenov, O. E.; Makeev, A. P.

    2014-11-01

    The pattern of the quasibiennial oscillation of total ozone over northern territories of Russia (1996-2013) and ozone concentrations at separate stratospheric levels over Arctic sites (2005-2013) are analyzed according to data of TOMS satellite instrumentation. It is shown that the entire period of 1996-2013 can be divided into three intervals: before 2002- 2004, interval between 2002-2004 and 2008-2010, and period after 2008-2010. The quasibiennial oscillation is quite clearly manifested in the first and third periods and is distorted in the second period. The time series of the mixing ratio, composed for separate altitudinal levels, exhibit quasibiennial oscillation, which takes shape at heights of ~30 km and weakens in overlying regions.

  3. Rapid disinfection of E-Coliform contaminated water using WO3 semiconductor catalyst by laser-induced photo-catalytic process.

    Gondal, Mohammed A; Khalil, Amjad

    2008-04-01

    Laser-induced photo-catalysis process using WO(3) semiconductor catalyst was applied for the study of disinfection effectiveness of E-coliform-contaminated water. For this purpose, wastewater polluted with E-coliform bacteria was exposed to 355 nm UV radiations generated by third harmonic of Nd: YAG laser in special glass cell with and without WO(3) catalyst. E-Coliform quantification was performed by direct plating method to obtain the efficiency of each disinfection treatment. The dependence of disinfection process on laser irradiation energy, amount of catalyst and duration of laser irradiation was also investigated. The disinfection with WO(3) was quite efficient inactivating E-coliforms. For inactivation of E-coliforms, less than 8 minutes' laser irradiation was required, so that, the treated water complies with the microbial standards for drinking water. This study opens the possibility of application of this simple method in rural areas of developing countries using solar radiation. PMID:18324535

  4. A Downflow Hanging Sponge (DHS) reactor for faecal coliform removal from an Upflow Anaerobic Sludge Bed (UASB) effluent

    Yaya Beas, R.E.; Kujawa-Roeleveld, K.; Lier, van J.B.; Zeeman, G.

    2015-01-01

    This research was conducted to study the faecal coliforms removal capacity of Downflow Hanging Sponge (DHS) reactors as a post-treatment for an Upflow Anaerobic Sludge Blanket (UASB) reactor. Three long-term continuous lab-scale DHS reactors i.e. a reactor with cube type sponges without recirculatio

  5. Modification of the standard most-probable-number procedure for fecal coliform bacteria in seawater and shellfish.

    Dexter, F.

    1981-01-01

    To determine whether or not presumptive tubes which take 48 h to become positive need to be transferred to EC medium during the fecal coliform multitube most-probable-number procedure, 572 seawater and shellfish samples were considered. When the 24-hour positive tubes alone were transferred to EC, it appeared that incorrect dilution data would be rare.

  6. Genomic breeding value prediction for simple maize hybrid yield using total effects of associated markers, under different imbalance levels and environments.

    Cantelmo, N F; Von Pinho, R G; Balestre, M

    2016-01-01

    The main objective of a maize breeding program is to generate hybrid combinations that are more productive than those pre-existing in the market. However, the number of parents, and consequently the number of crosses, increases so rapidly that the phenotypic evaluation of all the possible combinations becomes economically and technically infeasible. In this context, predicting the performance of the most promising genotypes may increase the genetic gains with increased selection intensity and reduced breeding cycles. Thus, the present study aimed to use the total effects of associated markers method to predict genomic breeding values (GBVs) via cross-validation and by using different imbalance levels (10, 30, 50, and 70%). A set of 51 genotyped strains was used with 79 microsatellite markers and 273 hybrids that were generated by a partial diallel. A total of 186 and 272 hybrids were analyzed in the experiments within the southern and central regions of Brazil, respectively. The GBVs were, thus, predicted for each location in both the regions, and for training in one region and validation in another region. The correlation between the predicted and observed GBVs ranged from 0.48 to 0.91, depending on the imbalance level and the region analyzed. Overall, the results obtained in the present study were promising, particularly considering that a small number of markers were used and that the training and predictions occurred in the very distinct regions of southern and central Brazil. PMID:26985952

  7. Effect of variable power levels on the yield of total aerosol mass and formation of aldehydes in e-cigarette aerosols.

    Gillman, I G; Kistler, K A; Stewart, E W; Paolantonio, A R

    2016-03-01

    The study objective was to determine the effect of variable power applied to the atomizer of refillable tank based e-cigarette (EC) devices. Five different devices were evaluated, each at four power levels. Aerosol yield results are reported for each set of 25 EC puffs, as mass/puff, and normalized for the power applied to the coil, in mass/watt. The range of aerosol produced on a per puff basis ranged from 1.5 to 28 mg, and, normalized for power applied to the coil, ranged from 0.27 to 1.1 mg/watt. Aerosol samples were also analyzed for the production of formaldehyde, acetaldehyde, and acrolein, as DNPH derivatives, at each power level. When reported on mass basis, three of the devices showed an increase in total aldehyde yield with increasing power applied to the coil, while two of the devices showed the opposite trend. The mass of formaldehyde, acetaldehyde, and acrolein produced per gram of total aerosol produced ranged from 0.01 to 7.3 mg/g, 0.006 to 5.8 mg/g, and aerosols from specific devices, and were compared to estimated exposure from consumption of cigarettes, to occupational and workplace limits, and to previously reported results from other researchers. PMID:26743740

  8. Effect of Pistacia Atlantica Extract on Glutathione Peroxidase Tissue Levels and Total Oxidative Capacity of Liver and Plasma Lipid Profile of Rats

    Parvin Farzanegi

    2013-11-01

    Full Text Available Background: Exercise causes increased oxygen consumption, leaving cells exposed to oxidative stress. Antioxidants may have a protective effect by inhibiting lipid peroxidation. Thus, this study aims to examine the effect of Pistacia atlantica extract on glutathione peroxidase levels and total oxidative capacity of liver and plasma lipid profile of rats. Materials and Methods: In this experimental study, 28 female rats’ weight 155.8±2.7 grams were randomly and equally divided into 4 groups of exercise-saline, control-saline, exercise-mastic, and control-mastic. The exercise groups exercised for 8 weeks (5 days per week, 60 minutes daily, 25 meters per minute, on a zero degree slope. The rats received equal volumes of mastic and saline orally for 4 weeks. Blood and tissue samples were taken 72 hours after the last exercise session. Data were analyzed using one-way variance analysis (ANOVA.Results: Consumption of Pistacia atlantica extract together with endurance exercising for 8 weeks did not significantly affect glutathione peroxidase concentration, total oxidative capacity, LDL, triglyceride, or cholesterol, but significantly reduced HDL (p=0.002.Conclusion: Results showed that antioxidant and lipid profile levels were not affected by consumption of supplements and endurance exercising. However, further studies are required to assess the long term effects of this herbal extract.

  9. Association of dietary fiber intake with serum total cholesterol and low density lipoprotein cholesterol levels in Urban Asian-Indian adults with type 2 diabetes

    Shreya Narayan

    2014-01-01

    Full Text Available Context: There is little data correlating dietary fibre (DF intake and cardiovascular risk in Asian Indians with diabetes. Aim: To assess the DF intake and its association with lipid profile (total serum cholesterol and low density lipoprotein [LDL] - cholesterol levels in urban Asian Indians with diabetes. Subjects and Methods: Dietary assessment using validated Food Frequency Questionnaire was conducted in 1191 free-living adults with known diabetes in the Chennai Urban Rural Epidemiology Study. Subjects taking medication for dyslipidemia, and those with cardiovascular disease and implausible energy intake (n = 262 were excluded, leaving 929 participants. Anthropometric and relevant biochemical parameters were measured using standardized techniques. Results: Diabetic individuals who consumed DF median intake of DF group. The risk of hypercholesterolemia (odds ratio [OR] =1.38 [95% confidence interval [CI]: 1.02-1.85], P = 0.04, and high LDL cholesterol (OR: 1.43 [95% CI: 1.06-1.94], P = 0.02 was higher among those whose DF intake was less than the median. Serum triglycerides and high density lipoprotein cholesterol were not associated with DF intake. The main sources of DF were vegetables and legumes. Conclusion: In urban Asian Indians with diabetes, lower DF intake is positively related to total cholesterol and LDL cholesterol levels.

  10. Total protein

    The total protein test measures the total amount of two classes of proteins found in the fluid portion of your ... nutritional problems, kidney disease or liver disease . If total protein is abnormal, you will need to have more ...

  11. Total protein

    ... page: //medlineplus.gov/ency/article/003483.htm Total protein To use the sharing features on this page, please enable JavaScript. The total protein test measures the total amount of two classes ...

  12. On the road to metallic nanoparticles by rational design: bridging the gap between atomic-level theoretical modeling and reality by total scattering experiments

    Prasai, Binay; Wilson, A. R.; Wiley, B. J.; Ren, Y.; Petkov, Valeri

    2015-10-01

    The extent to which current theoretical modeling alone can reveal real-world metallic nanoparticles (NPs) at the atomic level was scrutinized and demonstrated to be insufficient and how it can be improved by using a pragmatic approach involving straightforward experiments is shown. In particular, 4 to 6 nm in size silica supported Au100-xPdx (x = 30, 46 and 58) explored for catalytic applications is characterized structurally by total scattering experiments including high-energy synchrotron X-ray diffraction (XRD) coupled to atomic pair distribution function (PDF) analysis. Atomic-level models for the NPs are built by molecular dynamics simulations based on the archetypal for current theoretical modeling Sutton-Chen (SC) method. Models are matched against independent experimental data and are demonstrated to be inaccurate unless their theoretical foundation, i.e. the SC method, is supplemented with basic yet crucial information on the length and strength of metal-to-metal bonds and, when necessary, structural disorder in the actual NPs studied. An atomic PDF-based approach for accessing such information and implementing it in theoretical modeling is put forward. For completeness, the approach is concisely demonstrated on 15 nm in size water-dispersed Au particles explored for bio-medical applications and 16 nm in size hexane-dispersed Fe48Pd52 particles explored for magnetic applications as well. It is argued that when ``tuned up'' against experiments relevant to metals and alloys confined to nanoscale dimensions, such as total scattering coupled to atomic PDF analysis, rather than by mere intuition and/or against data for the respective solids, atomic-level theoretical modeling can provide a sound understanding of the synthesis-structure-property relationships in real-world metallic NPs. Ultimately this can help advance nanoscience and technology a step closer to producing metallic NPs by rational design.The extent to which current theoretical modeling alone can

  13. Presence of faecal coliforms and selected heavy metals in ice cubes from food outlets in Taman Universiti, Johor Bahru, Malaysia.

    Mahat, N A; Meor Ahmad, Z; Abdul Wahab, R

    2015-09-01

    Consumption of iced beverages is common in Malaysia although specific research focusing on its safety parameters such as presence of faecal coliforms and heavy metal elements remains scarce. A study conducted in Kelantan indicated that faecal coliforms were detected in the majority of the ice cube samples analyzed, largely attributable to improper handling. Hence, it was found pertinent to conduct similar study in other parts of the country such as Johor Bahru if the similar pattern prevailed. Therefore, this present cross sectional study which randomly sampled ice cubes from 30 permanent food outlets in Taman Universiti, Johor Bahru for detecting contamination by faecal coliforms and selected heavy metal elements (lead, copper, manganese and zinc) acquires significance. Faecal coliforms were detected in 11 (36.67%) of the samples, ranging between 1 CFU/100 mL to > 50 CFU/100 mL; two of the samples were grossly contaminated (>50 CFU/100 mL). Interestingly, while positive detection of lead was observed in 29 of the 30 ice cube samples (mean: 0.511±0.105 ppm; range: 0.489-0.674 ppm), copper, manganese and zinc were not detected. In addition, analysis on commercially bottled mineral water as well as in tap water samples did not detect such contaminations. Therefore, it appears that (1) contamination of faecal coliforms in ice cubes in food outlets in Malaysia may not be sporadic in pattern but rather prevalent and (2) the source of water used for manufacturing the ice cubes that contained significant amount of lead would suggest that (3) it was neither originated from the treated tap water supply nor bottled mineral water or (4) perhaps contaminated during manufacturing process. Further studies exploring the source of water used for manufacturing these ice cubes as well as the handling process among food operators deserve consideration. PMID:26695207

  14. Combined effects of DNA methyltransferase 1 and 3A polymorphisms and urinary total arsenic levels on the risk for clear cell renal cell carcinoma.

    Yang, Shu-Mei; Huang, Chao-Yuan; Shiue, Horng-Sheng; Pu, Yeong-Shiau; Hsieh, Yi-Hsun; Chen, Wei-Jen; Lin, Ying-Chin; Hsueh, Yu-Mei

    2016-08-15

    Our previous study showed that high urinary total arsenic levels were associated with higher odds ratio (OR) for renal cell carcinoma (RCC). Single nucleotide polymorphisms (SNPs) of DNA methyltransferases (DNMTs) might influence DNMT enzyme activity associated with tumorigenesis. In this study, we investigated the association of five SNPs from DNMT1 (rs8101626 and rs2228611), DNMT3A (rs34048824 and rs1550117), and DNMT3B (rs1569686) with the risk of clear cell renal cell carcinoma (ccRCC). We also examined the combined effects of DNMT genotypes and urinary arsenic levels on ccRCC risk. We conducted a hospital-based case-control study, which included 293 subjects with ccRCC and 293 age- and gender-matched controls. The urinary arsenic species were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Genotypes were investigated using polymerase chain reaction and restriction fragment length polymorphism analyses. We observed that the DNMT1 rs8101626 G/G genotype was significantly associated with reduced odds ratio (OR) of ccRCC [OR=0.38, 95% confidence interval (CI) 0.14-0.99]. Subjects with concurrent DNMT1 rs8101626 A/A+A/G and DNMT3A rs34048824 T/T+T/C genotypes had significantly higher OR for ccRCC [OR=2.88, 95% CI 1.44-5.77]. Participants with the high-risk genotype of DNMT1 rs8101626 and DNMT3A rs34048824 with concurrently high urinary total arsenic levels had even higher OR of ccRCC in a dose-response manner. This is the first study to evaluate variant DNMT1 rs8101626 and DNMT3A rs34048824 genotypes that modify the arsenic-related ccRCC risk in a geographic area without significant arsenic exposure in Taiwan. PMID:27292127

  15. Mosapride citrate, a 5-HT₄ receptor agonist, increased the plasma active and total glucagon-like peptide-1 levels in non-diabetic men.

    Aoki, Kazutaka; Kamiyama, Hiroshi; Masuda, Kiyomi; Togashi, Yu; Terauchi, Yasuo

    2013-01-01

    Mosapride citrate, a selective agonist of the 5-hydroxytryptaine (5-HT)₄ receptor, is typically used to treat heartburn, nausea, and vomiting associated with chronic gastritis or to prepare for a barium enema X-ray examination. Mosapride citrate reportedly improves insulin sensitivity in patients with type 2 diabetes. As mosapride citrate activates the motility of the gastrointestinal tract, we hypothesized that mosapride citrate affects incretin secretion. We examined the effect of the administration of mosapride citrate on the plasma glucose, serum insulin, plasma glucagon, and plasma incretin levels before breakfast and at 60, 120, and 180 min after breakfast in men with normal glucose tolerance (NGT) or impaired glucose tolerance (IGT) to exclude gastropathy. Mosapride citrate was administered according to two different intake schedules (C: control (no drug), M: mosapride citrate 20 mg) in each of the subject groups. The area under the curve (AUC) of the plasma glucose levels was smaller in the M group than in the C group. The time profiles for the serum insulin levels at 60 and 120 min after treatment with mosapride citrate tended to be higher, although the difference was not statistically significant. The AUCs of the plasma active and total glucagon-like peptide-1 (GLP-1) levels were significantly larger in the M group than in the C group. No significant difference in the AUC of the plasma glucose-dependent insulinotropic polypeptide (GIP) level was observed between the two groups. Our results suggest that mosapride citrate may have an antidiabetic effect by increasing GLP-1 secretion. PMID:23257734

  16. Estimation of serum, salivary immunoglobulin G, immunoglobulin A levels and total protein, hemoglobin in smokeless tobacco chewers and oral submucous fibrosis patients

    Chandrakanth Balakrishnan

    2015-01-01

    Full Text Available Background: Oral submucous fibrosis (OSMF is a debilitating, potentially cancerous oral condition. Although areca nut is the most important causative agent, it is also considered that the disease is immunologically mediated. Aim of the Study: To establish that autoimmunity and nutritional deficiency play a role in the etiopathogenesis of OSMF. Objectives of the Study: To show that serum immunoglobulin markers (immunoglobulin-G [IgG], immunoglobulin-A [IgA] and nutritional parameters such as total serum protein (TSP, Hemoglobin (Hb play a role in causing OSMF and also to correlate serum, salivary IgG, IgA levels in OSMF patients. Settings and Design: A case-control study was done with 50 patients (25 patients who were provisionally diagnosed as OSMF - Group I, and 25 patients who were chronic smokeless tobacco chewers and who did not have any intraoral lesion - Group II. Materials and Methods: Five milliliters of blood and saliva were collected from both the groups. Quantitative analysis of serum, and salivary IgG, IgA was done by turbidometric immunoassay. TSP and Hemoglobin (Hb were estimated by spectrophotometry. Statistical Analysis: Results were analyzed by independent samples t-test and one-way analysis of variance (ANOVA. Results: All patients of OSMF showed significant (P < 0.01 increase in serum IgG, IgA, and salivary IgG levels as compared to smokeless tobacco chewers. The salivary IgA levels showed a significant decrease in OSMF patients (P < 0.05. TSP and Hb levels showed significant (P < 0.01 decrease in OSMF patients as compared to smokeless tobacco chewers. Conclusion: The elevation of immunoglobulin levels supports the concept of autoimmunity. The decrease in TSP and Hb suggests that nutritional deficiency plays a defined role in the occurrence as well as a further progression of OSMF.

  17. 钱塘江(杭州段)粪大肠菌群污染现状研究%Analysis of pollution of fecal coliform in Qiantang river ( Hangzhou section)

    詹丽

    2011-01-01

    Objective: In the view of hygiene, through the continuous dynamic monitoring of fecal coliform in surface water of Qiantang River (Hangzhou section) in different seasons, different sections and different tidal conditions, to explore the factors of fecal coliform pollution, in order to provide a scientific basis to strengthen the control of Qiantang River water pollution. Methods: According to "Water and Wastewater Monitoring and Analysis Methods" (4th edition) of National Environmental Protection Administration, fecal coliform in surface water of Qiantang River( Hangzhou section) was monitored. Results-. According to the surface water M level standard for the fecal coliform, the average attainment rate is 61.0%. From 2006 to 2009, the attainment rates of fecal coliform in four years were 54.2% , 56.9% , 59.4% and 74.0% , showing a gradual uptrend. The attainment rate of fecal coliform in upstream river water is higher than that of downstream, and the attainment rates in four seasons reduced accordingly in the order of winter, spring, autumn and summer. Conclusion: The pollution of fecal coliform in Qiantang River ( Hangzhou section) is still serious, increasing year by year. The condition of pollution was increasingly improved, but the new pollution continue to breed. Strengthening the regulation of pollution sources and dynamic monitoring of water are still the focus of our work.%目的:从卫生学角度出发,通过对钱塘江(杭州段)流域地表水不同季节、不同河段、不同潮汐等条件粪大肠菌群连续动态监测,探究其粪大肠菌群污染的诸多因素,为加强钱塘江水体污染监管提供科学的依据.方法:按照国家环保局《水和废水监测分析方法》第四版要求,进行粪大肠菌群的监测.结果:以粪大肠菌群Ⅲ级地表水标准,平均达标率为61.0%,2006年- 2009年粪大肠菌群四年达标率依次为54.2%、56.9%、59.4%、74.0%,呈逐渐上升趋势.河段上游水体粪大肠菌

  18. Determination of calcium, potassium, manganese, iron, copper and zinc levels in representative samples of two onion cultivars using total reflection X-ray fluorescence and ultrasound extraction procedure

    The chemical characterization of onion cultivar samples is an important tool for the enhancement of their productivity due to the fact that chemical composition is closed related to the quality of the products. A new sample preparation procedure for elemental characterization is proposed, involving the acid extraction of the analytes from crude samples by means of an ultrasonic bath, avoiding the required digestion of samples in vegetable tissue analysis. The technique of total reflection X-ray fluorescence (TXRF) was successfully applied for the simultaneous determination of the elements Ca, K, Mn, Fe, Cu and Zn. The procedure was compared with the wet ashing and dry ashing procedures for all the elements using multivariate analysis and the Scheffe test. The technique of flame atomic absorption spectrometry (FAAS) was employed for comparison purposes and accuracy evaluation of the proposed analysis method. A good agreement between the two techniques was found when using the dry ashing and ultrasound leaching procedures. The levels of each element found for representative samples of two onion cultivars (Yellow Granex PRR 502 and 438 Granex) were also compared by the same method. Levels of K, Mn and Zn were significantly higher in the 438 Granex cultivar, while levels of Ca, Fe and Cu were significantly higher in the Yellow Granex PRR 502 cultivar

  19. Emission of SO2 and SO42- from copper smelter and its influence on the level of total s in soil and moss in Bor and the surroundings

    Šerbula Snežana M.

    2015-01-01

    Full Text Available Bor and the surroundings (Eastern Serbia have been known for exploitation and processing of sulphide copper ores for more than 100 years. Emissions of waste gases and particulate matter rich in heavy metals are characteristic for pyrometallurgical production of copper. Long-term measurement results (2005-2008 indicate an increased sulphur dioxide level in the urban-industrial zone of Bor since it is closest to the copper smelter which is a dominant source of air pollution in the studied area. Average annual sulphur dioxide concentrations at four measuring sites in the urban-industrial zone exceeded the maximum allowable value of 50 μg/m3. However the maximum allowable value of the total atmospheric depositions (200 mg/m2/day on an annual basis exceeded only at two of 15 measuring sites in the urban-industrial and rural zone. The highest annual deposition rate of sulphates from deposition was detected in the urban-industrial zone. Since the maximum permitted value for sulphates is not defined by the Serbian Regulations, the extent of the pollution cannot be discussed. Since the environment can continuously be polluted through the wet and dry deposition, biomonitoring by moss was conducted, which revealed significantly higher concentrations of total sulphur in moss in the urban-industrial zone, compared to the background zone. The obtained results confirm the reliability of moss as a bioindicator of ambient pollution. Higher total S concentration in soil samples was noted at the rural site (Ostrelj located in the close vicinity of two tailing ponds. [Projekat Ministarstva nauke Republike Srbije, br. 46010, br. 33038 i br. 172037

  20. Overview of total system model used for the 2008 performance assessment for the proposed high-level radioactive waste repository at Yucca Mountain, Nevada

    A summary is presented for the total system model used to represent physical processes associated with the seven scenario classes (i.e., nominal conditions, early waste package (WP) failure, early drip shield (DS) failure, igneous intrusive events, igneous eruptive events, seismic ground motion events and seismic fault displacement events) considered in the 2008 performance assessment for the proposed repository for high-level radioactive waste at Yucca Mountain, Nevada. The total system model estimates dose to an exposed individual resulting from radionuclide movement through the repository system and biosphere. Components of the total system model described in this presentation include models for (i) climate analysis, (ii) land surface infiltration and associated unsaturated zone flow, (iii) multi-scale thermal hydrology and engineered barrier system (EBS) thermal–hydrologic environment, (iv) EBS physical and chemical environment, (v) WP and DS degradation, (vi) drift seepage and drift wall condensation, (vii) waste form degradation and mobilization, (viii) water and radionuclide movement in the EBS and underlying unsaturated and saturated zones, (ix) radionuclide movement in the biosphere and resultant human exposure, and (x) processes specific to early WP and DS failures, intrusive and eruptive igneous events, and seismic ground motion and fault displacement events. - Highlights: • The models indicated below associated with four scenario classes are described: (i) nominal conditions, (ii) early waste package (WP) and/or early drip shield (DS) failure, (iii) igneous events, and (iv) seismic events. • Models for (i) multi-scale thermal hydrology and engineered barrier system (EBS) thermal-hydrologic environment, (ii) EBS physical and chemical environment, (iii) WP and DS degradation, (iv) drift seepage and drift wall condensation, and (vi) waste form degradation and mobilization. • Models for processes specific to (i) early WP and DS failures, (ii

  1. Estimation of total as well as bioaccessible levels and average daily dietary intake of iodine from Japanese edible seaweeds by epithermal neutron activation analysis

    An epi-thermal instrumental neutron activation analysis (EINAA) method in conjunction with Compton suppression spectrometry (EINAA-CSS) was used for the determination of total iodine in eight different species of edible seaweeds from Japan. This method gave an absolute detection limit of about 2 μg. The accuracy of the method was evaluated using various reference materials and found to be generally in agreement within ±6% of the certified values. The longitudinal distributions of iodine at different growing stages in Japanese sea mustard and tangle seaweeds were investigated. For a 150-cm-high tangle, the highest concentration (5,360 mg/kg) of iodine was found at the root, then decreased slowly to 780 mg/kg in the middle portion (60-75 cm), and increased to 2,300 mg/kg at the apex. On the other hand, for a 190-cm-high sea mustard the highest levels of iodine were found both at the roots (164 mg/kg) and apex (152 mg/kg) with lower values (98 mg/kg) in the middle section. In order to estimate the bioaccessible fraction of iodine, seaweeds were digested by an in vitro enzymolysis method, dietary fibre separated from residue, and both fractions analyzed by EINAA-CSS. The average daily dietary intakes of total (0.14 mg) as well as bioaccessible fraction (0.12 mg) of iodine from the consumption of sea mustards were estimated. (author)

  2. Clinical and radiologic comparison of dynamic cervical implant arthroplasty and cervical total disc replacement for single-level cervical degenerative disc disease.

    Shichang, Liu; Yueming, Song; Limin, Liu; Lei, Wang; Zhongjie, Zhou; Chunguang, Zhou; Xi, Yang

    2016-05-01

    Anterior cervical discectomy and fusion, to date the most successful spine procedure for the surgical treatment of cervical radiculopathy, has limitations that have led to the development of non-fusion cervical procedures, such as cervical total disc replacement (TDR) and dynamic cervical implant (DCI) arthroplasty. We compared the clinical and radiological results of DCI and cervical TDR for the treatment of single-level cervical degenerative disc disease in Chinese patients. A retrospective review of 179 patients with cervical spondylotic myelopathy who underwent DCI or TDR between April 2010 and October 2012 was conducted, and 152 consecutive patients (67 patients single-level DCI and 85 single-level TDR) who completed at least 2years of follow-up were included. Clinical and radiological assessments were performed preoperatively and at 1week and 3, 6, 12, and 24months postoperatively. The most common operative level was C5/C6 (49.3%). The differences in blood loss, duration of surgery, and duration of hospitalization were not statistically significant. The Japanese Orthopaedic Association scale, Visual Analog Scale, Neck Disability Index, and Short Form-36 scores improved significantly after surgery in both the DCI and TDR groups (P<0.05), but the differences were not statistically significant at the final follow-up. The rate of occurrence of heterotopic ossification was 22.4% and 28.2% in the DCI and TDR groups, respectively. As an effective non-fusion technique, DCI is a more economical procedure. Further prospective, randomized studies with long-term follow-up periods are needed to determine the long-term effects. PMID:26928156

  3. The Development of Low-Level Measurement Capabilities for Total and Isotopic Uranium in Environmental Samples at Brazilian and Argentine Laboratories by ABACC

    In June 1998, the Brazilian-Argentine Agency for Accounting and Control of Nuclear Materials (ABACC), with assistance from the U.S. Department of Energy (DOE), began a program to assess environmental sampling and analysis capabilities at laboratories in Argentina and Brazil. The program began with staff training conducted in South America and the United States by Oak Ridge National Laboratory (ORNL) and Pacific Northwest National Laboratory (PNNL). Both laboratories are participating members of DOE's Network of Analytical Laboratories (NWAL) that support IAEA's environmental sampling program. During the initial planning meeting, representatives from ABACC and all the participating analytical laboratories supporting ABACC were briefed on how the first exercise would be managed and on key aspects necessary to analyze low-level environmental samples for uranium. Subsequent to this training, a laboratory evaluation exercise (Exercise 1) was conducted using standard swipe samples prepared for this exercise by the International Atomic Energy Agency (IAEA). The results of Exercise 1 determined that sample contamination was a major factor in the analysis, and a thorough review of laboratory procedures was required to reduce the level of contamination to acceptable levels. Following modification of sample preparation procedures, the laboratories performed Exercise 2, an analysis of a National Institute of Standards and Technology (NIST) Standard Reference Material (SRM) 1547, Peach Leaves. The results of Exercise 2 demonstrated that several laboratories were capable of accurately determining the total uranium and uranium isotopic distribution in the peach leaves. To build on these successes, Exercise 3 was performed using a series of standard swipe samples prepared by the IAEA and distributed to laboratories supporting ABACC and to PNNL and ORNL. The results of Exercise 3 demonstrate that ABACC now has support laboratories in both Argentina and Brazil, which are capable of

  4. Total tract nutrient digestion and milk fatty acid profile of dairy cows fed diets containing different levels of whole raw soya beans.

    Venturelli, B C; de Freitas Júnior, J E; Takiya, C S; de Araújo, A P C; Santos, M C B; Calomeni, G D; Gardinal, R; Vendramini, T H A; Rennó, F P

    2015-12-01

    Whole oilseeds such as soya beans have been utilized in dairy rations to supply additional fat and protein. However, antinutritional components contained in soya beans, such as trypsin inhibitors and haemagglutinins (lectins) may alter digestibility of nutrients and consequently affect animal performance. The objective of the present experiment was to quantify the effect of different levels of whole raw soya beans in diets of dairy cows on nutrient intake, total tract digestion, nutrient balances and milk yield and composition. Sixteen mid to late-lactation cows (228 ± 20 days in milk; mean ± SD) were used in four replicated 4 × 4 Latin square design experiment with 21-d periods. Cows were assigned to each square according to milk yield and DIM. The animals were randomly allocated to treatments: control (without soya beans addition; CO), WS9, WS18 and WS27, with addition of 9%, 18% and 27% of whole raw soya bean in diet on a dry matter (DM) basis respectively. All diets contained identical forage and concentrate components and consisted of maize silage and concentrate based on ground corn and soya beans at a ratio of 60:40. There were no differences in OM, CP, NDF and NEL intakes (kg/day and MJ/day) among the treatments (p > 0.05). However, DM and NFC intakes were negatively affected (p = 0.04 and p < 0.01, respectively) and ether extract (EE) intake was positively affected (p < 0.01). Total tract digestion increased linearly with whole raw soya beans for EE (p < 0.01) and NDF (p = 0.01). The excretion (kg/day) of digested soya beans grains increased linearly according to addition of whole raw soya beans. However, the nutritive characteristics of excreted grains were not altered. Milk (kg), milk lactose (kg) and protein (kg) yield decreased linearly (p < 0.01, p < 0.01 and p = 0.04, respectively) milk fat content (%) increased linearly (p < 0.01) with whole raw soya beans inclusion. Increasing addition of whole raw soya beans affected milk fatty acid profile

  5. Organic and total mercury levels in bigeye tuna, Thunnus obesus, harvested by Taiwanese fishing vessels in the Atlantic and Indian Oceans.

    Chen, M H; Teng, P Y; Chen, C Y; Hsu, C C

    2011-01-01

    Muscle samples of 121 and 110 bigeye tuna (Thunnus obesus) caught by Taiwanese long-line fishing vessels in the Atlantic and Indian Oceans, respectively, were used to analyze total mercury (THg) and organic mercury (OHg) content. The overall THg and OHg concentrations were 0.786 ± 0.386 (0.214-3.133) and 0.595 ± 0.238 (0.143-2.222) mg kg⁻¹ wet weight, respectively, similar to the results of previous studies. Our findings, however, reflected the highest THg and OHg concentrations for the species in each ocean among the published data. Mean THg and OHg concentrations in Atlantic tuna were significantly (p tuna. Two of 121 samples of tuna from the Atlantic Ocean, but no samples from the Indian Ocean, had levels of OHg above 2 mg kg⁻¹ wet weight set by the Department of Health Taiwan, and 13 of 121 samples of tuna from the Atlantic Ocean and three of 110 samples from the Indian Ocean had levels of OHg above 1 mg kg⁻¹ wet weight set by US FDA and WHO. Accordingly, for adult Taiwanese men and women with average body weight of 65 and 55 kg, respectively, the maximum allowable weekly intake of bigeye tuna is suggested to be 170 and 145 g, respectively. PMID:24779657

  6. Assessment of total and organic vanadium levels and their bioaccumulation in edible sea cucumbers: tissues distribution, inter-species-specific, locational differences and seasonal variations.

    Liu, Yanjun; Zhou, Qingxin; Xu, Jie; Xue, Yong; Liu, Xiaofang; Wang, Jingfeng; Xue, Changhu

    2016-02-01

    The objective of this study is to investigate the levels, inter-species-specific, locational differences and seasonal variations of vanadium in sea cucumbers and to validate further several potential factors controlling the distribution of metals in sea cucumbers. Vanadium levels were evaluated in samples of edible sea cucumbers and were demonstrated exhibit differences in different seasons, species and sampling sites. High vanadium concentrations were measured in the sea cucumbers, and all of the vanadium detected was in an organic form. Mean vanadium concentrations were considerably higher in the blood (sea cucumber) than in the other studied tissues. The highest concentration of vanadium (2.56 μg g(-1)), as well as a higher degree of organic vanadium (85.5 %), was observed in the Holothuria scabra samples compared with all other samples. Vanadium levels in Apostichopus japonicus from Bohai Bay and Yellow Sea have marked seasonal variations. Average values of 1.09 μg g(-1) of total vanadium and 0.79 μg g(-1) of organic vanadium were obtained in various species of sea cucumbers. Significant positive correlations between vanadium in the seawater and V org in the sea cucumber (r = 81.67 %, p = 0.00), as well as between vanadium in the sediment and V org in the sea cucumber (r = 77.98 %, p = 0.00), were observed. Vanadium concentrations depend on the seasons (salinity, temperature), species, sampling sites and seawater environment (seawater, sediment). Given the adverse toxicological effects of inorganic vanadium and positive roles in controlling the development of diabetes in humans, a regular monitoring programme of vanadium content in edible sea cucumbers can be recommended. PMID:25732906

  7. Total algorithms

    Tel, G.

    1993-01-01

    We define the notion of total algorithms for networks of processes. A total algorithm enforces that a "decision" is taken by a subset of the processes, and that participation of all processes is required to reach this decision. Total algorithms are an important building block in the design of distri

  8. Ocorrência de Salmonella e coliformes de origem fecal na canela em pó (Cinnamomum cassia Blume a Cinnamomum zeylanicum Nees) comercializada em Florianópolis, Santa Catarina, Brasil Salmonella and fecal coliforms in cinnamon (Cinnamomum cassia Blume and Cinnamomum zeylanicum Nees) sold in the city of Florianópolis, Santa Catarina, Brazil

    Jane Maria de S. Philippi; Eliane Moretto

    1995-01-01

    Cem amostras de canela em pó de dez marcas diferentes comercializadas na cidade de Florianópolis, SC, foram submetidas à análise microbiológica, pesquisando-se Salmonella e coliformes de origem fecal. Em nenhuma amostra foi detectada Salmonella. Coliformes de origem fecal foram encontrados entre os valores

  9. Effect of elevated total cholesterol level and hypertension on the risk of fatal cardiovascular disease: a cohort study of Chinese steelworkers

    YANG Ying; GU Dong-feng; LI Jian-xin; CHEN Ji-chun; CAO Jie; LU Xiang-feng; CHEN Shu-feng; WU Xi-gui; DUAN Xiu-fang; MO Xing-bo

    2011-01-01

    Background Increased blood pressure and elevated total cholesterol (TC) level are the two most important modifiable risk factors of cardiovascular disease (CVD) in the world.Hypertension and hypercholesterolemia co-exist more often than would be expected and whether there is a synergistic impact on fatal CVD between elevated TC and hypertension need to be further examined in Chinese population.Methods We conducted a cohort study which recruited 5092 Chinese male steelworkers aged 18-74 years in 1974-1980 and followed up for an average of 20.84 years.Totally 302 fatal CVD events were documented by the year of 2001.Cox proportional hazards regression models were undertaken to adjust for baseline variables with fatal CVD events as the outcome variable.Additive interaction model was used to evaluate the interaction between elevated TC and hypertension.Results Hypercholesterolemia and hypertension were significantly associated with an increased hazard ratio (HR) of fatal CVD (1.67 (95% CI 1.18-2.38) and 2.91 (95% CI 2.23-3.80) respectively.Compared to participants with normotension and TC <240 mg/dl,the HRs were 1.11 (95% CI 0.56-2.21),2.74 (95% CI 2.07-3.64) for hypercholesterolemia and hypertension respectively,and 5.51 (95% CI 3.58-8.46) for participants with both risk factors.There was an additive interaction with a 2.65 (95% CI 0.45-4.85) relative excess risk (RERI) between hypercholesterolemia and hypertension on CVD.Conclusion We found that the risk of fatal CVD was significantly associated with an additive interaction due to hypercholesterolemia and hypertension besides a conventional main effect derived from either of them,which highlights that the prevention and treatment of both risk factors might improve the individual risk profile thus reduce the CVD mortality.

  10. A mechanistic model of runoff-associated fecal coliform fate and transport through a coastal lagoon.

    Steets, B M; Holden, P A

    2003-02-01

    Fecal coliform (FC) contamination in coastal waters is an ongoing public health problem worldwide. Coastal wetlands and lagoons are typically expected to protect coastal waters by attenuating watershed pollutants including FC bacteria. However, new evidence suggests that coastal lagoons or marshes can also be a source of high indicator organism concentrations in coastal waters. We asked for a Mediterranean-type climate, what is the fate of runoff-associated FC through a coastal lagoon? To address this question, we developed a mass balance-based, mechanistic model of FC concentration through a coastal lagoon and simulated, for summer and winter conditions, FC within the lagoon water column, lagoon sediments, and in the ocean water just downstream of the lagoon mouth. Our model accounts for advective flow and dispersion, decay and sedimentation and resuspension of FC-laden sediments during high flow, erosional conditions. Under low flow conditions that occur in the summer, net FC decay and FC storage in lagoon sediments are predicted. Under high flow conditions that occur in the winter, FC-laden sediments are predicted to erode, resuspend and flow out of the lagoon where they elevate FC concentrations in the coastal ocean. For both seasonal conditions, the predicted water column FC concentrations were within an order of magnitude of field measurements for a reference site in southern California. Our results suggest that there are seasonally varying roles for coastal lagoons in mediating FC contamination to coastal waters. PMID:12688694

  11. Microbiological analysis of coliforms and mesophilic aerobic spore formers in gamma irradiated cocoa beans (Theobroma cacao L.)

    The presence of coliforms in processed foods is an useful indicator of post-sanitization and post processing contamination, and members of the mesophilic aerobic spore formers have great importance in food spoilage. Spore - forming aerobic bacilli have been observed in fermenting cocoa in Jamaica and West Africa. The results of this work has shown a considerable reduction of the mesophilic aerobic spore formers in irradiated Brazilian Comun Cocoa beans as long as the irradiation dose was increased from 1.05 to 3.99 kGy. The presence of coliforms irradiated has not been found even in the coroa beans with the dose of 1.05 kGy. (author). 4 refs., 1 tab

  12. Ocorrência de Salmonella e coliformes de origem fecal na canela em pó (Cinnamomum cassia Blume a Cinnamomum zeylanicum Nees comercializada em Florianópolis, Santa Catarina, Brasil Salmonella and fecal coliforms in cinnamon (Cinnamomum cassia Blume and Cinnamomum zeylanicum Nees sold in the city of Florianópolis, Santa Catarina, Brazil

    Jane Maria de S. Philippi

    1995-12-01

    Full Text Available Cem amostras de canela em pó de dez marcas diferentes comercializadas na cidade de Florianópolis, SC, foram submetidas à análise microbiológica, pesquisando-se Salmonella e coliformes de origem fecal. Em nenhuma amostra foi detectada Salmonella. Coliformes de origem fecal foram encontrados entre os valores The microbiological quality of a hundred samples of ten differents commercial brands of a ground cinnamon (Cinnamomum cassia Blume and Cinnamomum zeylanicum Nees from supermarkets in the city of Florianópolis, Brazil, was assessed. Salmonella and fecal coliforms were determined. Results were negative for Salmonella Fecal coliforms values ranged from 100 MPN per g. Fecal coliforms were detected in 37% of the cinnamon samples.

  13. Survey of PAC Performance for Removal of Turbidity, COD, Coliform Bacteria, Heterotrophic Bacteria from Water of Karoon River

    N Alavi Bakhtiarvand; A Takdastan; Mirzaei, A

    2011-01-01

    Backgrounds and Objectives: Selection of proper coagulants for turbidity removal and determination of effective methods to reduce coagulants dose and related costs in water treatment plants is of critical importance. The present study investigates the effect of returned sludge on improving the performance of poly-aluminum chloride (PAC) in turbidity, coliform bacteria, heterotrophic bacteria removal from drinking water during rapid mixing phase. Materials and Methods: In order to determine th...

  14. Analisa Bakteri Coliform Pada Air Kran Di Rumah Makan Yang Terdapat Di Jalan Dr Mansur,Medan Tahun 2015

    Pandian, Sharmilla

    2016-01-01

    There are many restaurant that sell food and drinks around the Jalan dr Mansur area. Taking into account that there are many factors that contribute to the growth and cultivation of bacteria in the tap water, thus there is a high possibility of the water being poluted by bacteria. Coliform bacteria including Escherichia coli is an indicator of water polution by waste and is not healthy to consume bu human. Poluted water may be hazardous to human health and well being. The...

  15. Identifikasi Bakteri Coliform yang Terdapat pada Minuman Es Teh di Rumah Makan Tepi Laut Purus Padang Barat

    Muhammad Nurman Ariefiansyah

    2015-09-01

    Full Text Available Abstrak Minuman es teh merupakan minuman yang paling digemari konsumen rumah makan. Sampel penelitian ini adalah minuman es teh dirumah makan Tepi Laut Purus Padang Barat. Tujuan penelitian ini adalah menentukan kualitas minuman es teh di rumah makan Tepi Laut Purus Padang Barat secara mikrobiologis. Sampel diambil dari 14 rumah makan Tepi Laut Purus Padang Barat. Penelitian dilaksanakan dua tahap; 1. pengambilan sampel minuman es teh disertai observasi dan wawancara, 2. pemeriksaan mikrobiologis dengan metode Most Propable Number (MPN terhadap sampel yang terdiri dari tes presumtif, tes konfirmatif dan tes pelengkap. Hasil penelitian mendapatkan seluruh sampel minuman es teh mengandung bakteri Coliform. Tiga belas dari empat belas sampel positif mengandung E. coli, sedangkan satu sampel lain mengandung bakteri Klebsiella. Dari penelitian ini dapat disimpulkan minuman es teh di rumah makan Tepi Laut Purus Padang Barat tidak memenuhi syarat mikrobiologis yang telah ditetapkan pemerintah.Kata kunci: minuman es teh, bakteri coliform, most propable number (MPN Abstract Ice tea is the common consumer favorite beverage at restaurant. Samples of this research is ice tea beverages at Purus Beach restaurant West Padang. The objective of this study was to identify the quality of ice tea sold at Purus Beach Restaurant in Purus West Padang microbiological. Samples were taken from restaurant of Purus Beach in West Padang. This research implemented in two steps; First, taking the ice tea sample and observing also interviewing. Second, microbiological inspection using Most Propable Number (MPN methods to the sample consist of presumptivetest, confirmation test and complementary test. All of the sample of the ice tea beverage containing Coliform bacteria. Thirteen of fourteen positive contained E.Coli and the other contain Clebsiella. This result showed that ice tea from Purus Beach West Padang restaurant is unqualified as government has set required

  16. Calculation of the expected increases of coliform organisms, Escherichia coli and Salmonella typhimurium, in raw blended mutton tissue.

    Smith, M.G.

    1987-01-01

    Samples of blended mutton tissue in small polyvinyl chloride sachets were incubated in water baths for different times and at varying temperatures. The temperature of each bath was recorded accurately throughout each experiment. Using equations previously derived for the lag and generation times of coliform organisms in blended mutton tissue, the expected increases of these bacteria were calculated from the time/temperature recordings. These were compared with the data obtained from plate cou...

  17. Comparison of methods of sampling for Toxocara species and fecal coliforms in an outdoor day care environment

    Carabin, Hélène; Gyorkos, Theresa W.; Kokoskin, Evelyne; Payment, Pierre; Joseph, Lawrence; Soto, Julio

    1998-01-01

    OBJECTIVE: To compare three sampling methods and to pretest methods for the determination of fecal coliform (FC) counts and Toxocara species from sand in the day care outdoor environment.DESIGN: The sand samples were obtained from the play area and the sandbox of a day care centre and examined for the presence of FC and Toxocara species, the common roundworm of dogs and cats. The sampling methods included random selection and two types of judgement methods. The latter included one method wher...

  18. Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: anatomic pathology, total blood cell count and serum protein levels

    KO Garcia

    2013-06-01

    Full Text Available The aim of the present study was to comparatively evaluate hemogram, blood serum components and anatomopathologic alterations in commercial layers experimentally challenged with an attenuated vaccine candidate strain (SG∆cobS∆cbiA and other two pathogenic strains (SGDcobS and SGNalr of Gallinarum (SG. In total, 280 commercial layers were randomly divided into 4 groups (G1, G2, G3 and G4. At five days of age, birds from groups G1 received approximately 107 colony forming units (CFU of SGDcobS; meanwhile birds from group G2 and G3 received the same dose of SGNalr and SG∆cobS∆cbiA, respectively. Birds from G4 were not infected. At 24 hours before (DBI and 24 hours after (1 DAI, and three (3 DAI, five (5 DAI, seven (7 DAI ten (10 DAI, and fifteen (15 DAI days after the infection, 10 birds of each group were humanely killed and blood samples collected to hematological and serum tests. Samples of liver, spleen, thymus, bursa of Fabricius, kidney and heart were also collected for the histological examination. Birds inoculated with SGDcobS and SGNalr showed similar alterations in hemogram, blood serum components and anatomopathologic exams. On the other hand, the exams of birds inoculated with SG∆cobS∆cbiA strain were similar to those of the uninfected birds. However, changes could be noticed in levels of uric acid and cholesterol during the course of the infection of birds from G3. Decrease in levels of light IgG 3 DAI was also observed in birds from this group. Pyknosis in kidney cells was a microscopic alteration found in birds from G3. Further studies must be done to verify if these alterations will not interfere in the performance of the vaccinate birds with SG∆cobS∆cbiA strain.

  19. Total mercury and methylmercury levels in pregnant women, nursing women and preschool children - residents of fisheries in the eighth region of Chile

    In the 1991-1993 period, efforts were concentrated on establishing and validating the analytical methodology for determining total mercury (Hg-T) and methylmercury (MeHg) in human hair, and to identify any high risk populations in the study group. Two surveys were conducted during this period, which involved the collection of scalp hair samples that were prepared and analyzed for Hg-T, and also for MeHg in selected samples. The mean hair Hg-T concentration determined in the study group (1.81 ± 1.52 mg/kg, as dry weight) was significantly higher than the level obtained in the control group (0.42 ± 0.15 mg/kg). These results were characterized according to geographical location of the FVs, frequency of fish and seafood consumption and residence period in the same FV. Multiple comparison tests confirmed significant differences between the arithmetic means obtained in each FV and the control group. Five of the FVs with higher Hg-T concentrations in PW and NW were selected for further and more in-depth studies. A new survey, which is now in progress, is also described, which targets these five FVs and the associated control group. (author)

  20. Ambient ozone effects on gas exchange and total non-structural carbohydrate levels in cutleaf coneflower (Rudbeckia laciniata L.) growing in Great Smoky Mountains National Park

    Ozone-sensitive and -tolerant individuals of cutleaf coneflower (Rudbeckia laciniata L.) were compared for their gas exchange characteristics and total non-structural carbohydrates at Purchase Knob, a high elevation site in Great Smoky Mountains National Park, USA. Photosynthesis and stomatal conductance decreased with increased foliar stipple. Sensitive plants had lower photosynthetic rates for all leaves, except the very youngest and oldest when compared to tolerant plants. Stomatal conductance decreased with increasing leaf age, but no ozone-sensitivity differences were found. Lower leaves had less starch than upper ones, while leaves on sensitive plants had less than those on tolerant plants. These results show that ambient levels of ozone in Great Smoky Mountains National Park can adversely affect gas exchange, water use efficiency and leaf starch content in sensitive coneflower plants. Persistence of sensitive genotypes in the Park may be due to physiological recovery in low ozone years. - Highlights: ► Ozone-sensitive and -tolerant cutleaf coneflower (Rudbeckia laciniata L.) were compared. ► Net photosynthesis decreased with increased foliar stipple and leaf age. ► Sensitive plants with visible stipple had lower photosynthetic rates when compared to tolerant plants. ► Stomatal conductance did not differ between sensitivity types. ► Sensitive plants had lower leaf starch content than tolerant plants. - Ambient ozone may cause declines in gas exchange and starch in ozone-sensitive genotypes of cutleaf coneflower.

  1. Serum total IgE levels and total eosinophil counts: relationship with treatment response in patients with acute asthma Relação dos níveis séricos de IgE total e das contagens de eosinófilos com a resposta ao tratamento em pacientes com asma aguda

    Ebrahim Razi

    2010-02-01

    Full Text Available OBJECTIVE: To determine whether serum total IgE levels and total eosinophil counts have any relationship with the response to routine pharmacological treatment in patients with acute asthma. METHODS: A cross-sectional study involving 162 patients with acute asthma. Serum total IgE levels, peripheral blood cell counts and eosinophil counts were determined. The treatment was adjusted for each patient according to the severity of asthma. Spirometry was performed at baseline and two weeks after the treatment. The patients were divided into two groups: high IgE (>100 IU/mL and low IgE (OBJETIVO: Determinar se há uma relação dos níveis de IgE total no soro e das contagens de eosinófilos com a resposta à farmacoterapia de rotina em pacientes com asma aguda. MÉTODOS: Estudo transversal com 162 pacientes com asma aguda. Foram determinados os níveis séricos de IgE total, as contagens de células no sangue periférico e as contagens de eosinófilos. O tratamento foi ajustado individualmente de acordo com a gravidade da asma. Foi realizada espirometria antes do início do tratamento e duas semanas após seu término. Os pacientes foram divididos em dois grupos: alto nível de IgE (> 100 UI/mL e baixo nível de IgE (< 100 UI/mL. Foram comparadas entre os dois grupos as relações das determinações basais e das alterações em percentual dos seguintes parâmetros: VEF, CVF, FEF25-75%, contagem de células brancas no sangue periférico e contagem de eosinófilos. RESULTADOS:Não houve diferenças significativas entre os grupos em relação às alterações em percentual dos parâmetros estudados. Tampouco houve diferenças significativas entre os grupos em relação aos valores basais de VEF, CVF e FEF25-75%, em % do predito. CONCLUSÕES: Com base nesses achados, concluímos que os níveis séricos de IgE total, as contagens de células brancas no sangue periférico e as contagens de eosinófilos não são preditores do tratamento farmacológico de

  2. Holding thermal receipt paper and eating food after using hand sanitizer results in high serum bioactive and urine total levels of bisphenol A (BPA.

    Annette M Hormann

    Full Text Available Bisphenol A (BPA is an endocrine disrupting environmental contaminant used in a wide variety of products, and BPA metabolites are found in almost everyone's urine, suggesting widespread exposure from multiple sources. Regulatory agencies estimate that virtually all BPA exposure is from food and beverage packaging. However, free BPA is applied to the outer layer of thermal receipt paper present in very high (∼20 mg BPA/g paper quantities as a print developer. Not taken into account when considering thermal paper as a source of BPA exposure is that some commonly used hand sanitizers, as well as other skin care products, contain mixtures of dermal penetration enhancing chemicals that can increase by up to 100 fold the dermal absorption of lipophilic compounds such as BPA. We found that when men and women held thermal receipt paper immediately after using a hand sanitizer with penetration enhancing chemicals, significant free BPA was transferred to their hands and then to French fries that were eaten, and the combination of dermal and oral BPA absorption led to a rapid and dramatic average maximum increase (Cmax in unconjugated (bioactive BPA of ∼7 ng/mL in serum and ∼20 µg total BPA/g creatinine in urine within 90 min. The default method used by regulatory agencies to test for hazards posed by chemicals is intra-gastric gavage. For BPA this approach results in less than 1% of the administered dose being bioavailable in blood. It also ignores dermal absorption as well as sublingual absorption in the mouth that both bypass first-pass liver metabolism. The elevated levels of BPA that we observed due to holding thermal paper after using a product containing dermal penetration enhancing chemicals have been related to an increased risk for a wide range of developmental abnormalities as well as diseases in adults.

  3. Circulating levels of osteopontin, osteoprotegerin, total soluble receptor activator of nuclear factor-kappa B ligand, and high-sensitivity C-reactive protein in patients with active rheumatoid arthritis randomized to etanercept alone or in combination with methotrexate

    Sennels, H.; Sørensen, Steen; Østergaard, Mikkel;

    2008-01-01

    OBJECTIVE: To determine whether circulating levels of osteopontin (OPN), osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-kappa B ligand (total sRANKL), and high-sensitivity C-reactive protein (hsCRP) change in patients with rheumatoid arthritis (RA) during...

  4. Enumeração de coliformes fecais e presença de Salmonella sp. em queijo Minas Enumeration of fecal coliforms and presense of Salmonella sp. in Minas cheese

    M.L. Pereira; M.C.A. Gastelois; E.M.A.F Bastos; Caiaffa, W. T.; E.S.C. Faleiro

    1999-01-01

    Foram submetidas à contagem de coliformes fecais e pesquisa de Salmonella sp. 168 amostras de variedades de queijo Minas (20 frescal, 48 canastra e 100 padronizado) coletadas em Belo Horizonte no período de 1995 a 1996. Foram adquiridos queijos com e sem registro no Serviço de Inspeção Federal (SIF) para avaliar a diferença de qualidade microbiológica. Os resultados evidenciaram ausência de Salmonella sp. em todas as amostras de queijo. Entretanto, verificou-se que 90% das amostras de queijo ...

  5. Digesta Passage Time, Digestibility, and Total Gut Fill in Captive Japanese Macaques (Macaca fuscata): Effects Food Type and Food Intake Level

    Sawada, Akiko; Sakaguchi, Ei; Hanya, Goro

    2011-01-01

    Digestion is an important process in understanding the feeding ecology of animals. We examined digesta passage time, digestibility, and total gut fill in Japanese macaques (Macaca fuscata; n = 4) under 4 dietary conditions representing the seasonal and regional variations in the diets of wild populations to determine the effects of food type and food intake on these digestive features. Food type is associated with mean retention time (MRT), digestibility, and total gut fill. Dry matter intake...

  6. In vitro evaluation of a novel bacteriophage cocktail as a preventative for bovine coliform mastitis.

    Porter, J; Anderson, J; Carter, L; Donjacour, E; Paros, M

    2016-03-01

    The objective of this study was to investigate the potential use of bacteriophage in preventing Escherichia coli mastitis on dairies. A cocktail consisting of 4 distinct bacteriophages was generated by screening against 36 E. coli isolates from dairy cows in Washington State with clinical mastitis. The bacteriophage significantly inhibited growth of 58% of the Washington State isolates and 54% of E. coli mastitis isolates from New York State, suggesting that the cocktail of phages had a relatively broad spectrum of action against relevant strains from 2 distinct geographies. The ability to suppress bacterial growth of these isolates in a liquid growth medium was not affected by the ratio of bacteriophage particles to bacterial cells (multiplicity of infection, MOI). For those E. coli that were completely inhibited by the phage cocktail, an MOI as low as 10had the same effect as 10µg/mL of ceftiofur on the growth rate of E. coli over a 12-h period using optical density measurements. A 3.3- to 5.6-log reduction of growth was achieved when E. coli was co-incubated with our phage cocktail in raw milk over a 12-h period at physiologic temperature. A modified gentamicin protection assay using bovine mammary epithelial cells provided a model to test whether bacteriophage could prevent cell attachment and invasion by chronic coliform mastitis strains. Pretreatment of cell cultures with the phage cocktail significantly reduced adhesion and intracellular survival of E. coli compared with controls. When combined with a bismuth-based teat sealant, the phage cocktail was able to inhibit bacterial growth when challenged with 1.6×10(3) cfu/mL of a clinical mastitis E. coli strain. In vitro results show bactericidal activity by our phage in raw milk and mammary tissue culture systems. Before a bacteriophage-based dry-cow treatment becomes a potential option for dairies, in vivo studies must be able to demonstrate that a specific dose of bacteriophage can protect cows from

  7. Low serum levels of free and total insulin-like growth factor I (IGF-I) in patients with anorexia nervosa are not associated with increased IGF-binding protein-3 proteolysis

    Støving, R K; Flyvbjerg, A; Frystyk, J;

    1999-01-01

    Patients with anorexia nervosa (AN) are GH resistant, with elevated GH levels and low serum levels of total insulin-like growth factor I (IGF-I). IGF-I action is modulated by IGF-binding proteins (IGFBPs), and a variety of catabolic states has been characterized by the presence of increased IGFBP-3...

  8. A downflow hanging sponge (DHS) reactor for faecal coliform removal from an upflow anaerobic sludge blanket (UASB) effluent.

    Yaya Beas, Rosa Elena; Kujawa-Roeleveld, Katarzyna; van Lier, Jules B; Zeeman, Grietje

    2015-01-01

    This research was conducted to study the faecal coliforms removal capacity of downflow hanging sponge (DHS) reactors as a post-treatment for an upflow anaerobic sludge blanket (UASB) reactor. Three long-term continuous laboratory-scale DHS reactors, i.e. a reactor with cube type sponges without recirculation, a similar one with recirculation and a reactor with curtain type sponges, were studied. The porosities of the applied medium were 91%, 87% and 47% respectively. The organic loading rates were 0.86 kgCOD m(-3) d(-1), 0.53 kgCOD m(-3) d(-1) and 0.24 kgCOD m(-3) d(-1) correspondingly at hydraulic loading rates of 1.92 m3 m(-2) d(-1), 2.97 m3 m(-2) d(-1) and 1.32 m3 m(-2) d(-1), respectively (COD: chemical oxygen demand). The corresponding averages for faecal coliform removal were 99.997%, 99.919% and 92.121% respectively. The 1989 WHO guidelines standards, in terms of faecal coliform content for unrestricted irrigation (category A), was achieved with the effluent of the cube type DHS (G1) without recirculation. Restricted irrigation, category B and C, is assigned to the effluent of the cube type with recirculation and the curtain type, respectively. Particularly for organic compounds, the effluent of evaluated DHS reactors complies with USEPA standards for irrigation of so called non-food crops like pasture for milking animals, fodder, fibre, and seed crops. PMID:26606098

  9. Total parenteral nutrition - infants

    Total parenteral nutrition (TPN) is a method of feeding that bypasses the gastrointestinal tract. Fluids are given into a vein to ... babies. It can provide a better level of nutrition than regular intravenous (IV) feedings, which provide only ...

  10. Ambient ozone effects on gas exchange and total non-structural carbohydrate levels in cutleaf coneflower (Rudbeckia laciniata L.) growing in Great Smoky Mountains National Park

    Ozone-sensitive and -tolerant individuals of the perennial herbaceous cutleaf coneflower (Rudbeckia laciniata L.) were compared for their gas exchange characteristics and total non-structural carbohydrates in the Great Smoky Mountains National Park USA. Net photosynthesis decreased with increased f...

  11. Effect of varying levels of formaldehyde treatment of mustard oil cake on rumen fermentation, digestibility in wheat straw based total mixed diets in vitro

    Mahima,; Vinod Kumar; Tomar, S. K.; Debashis Roy; Muneendra Kumar

    2015-01-01

    Aim: The aim of the current study was to protect the protein in mustard cake by different levels of formaldehyde treatment with a view to optimize the level of formaldehyde. Materials and Methods: Different levels of formaldehyde treatment (0, 1, 1.5 and 2% of crude protein) containing concentrate and roughages diet in 40:60 ratio were tested for their effect on nutrients digestibility, in vitro ammonia release, in vitro gas production and change in protein fractions. Non-significant (p≤0.05)...

  12. Number of residual thermotolerant coliforms on plants and in soil when using reclaimed domestic wastewater for irrigation.

    Khamkure, Sasirot; Cervantes, Edmundo Peña; Zermeño González, Alejandro; Cervantes, Rubén López; Melo, Prócoro Gamero; Ramírez, Homero

    2013-01-01

    The reclamation of domestic wastewater for irrigation is one alternative approach to solve the water scarcity crisis, but it is essential to control the microbiological quality of wastewater used for irrigation. The removal of thermotolerant coliforms, also known as faecal coliforms (FC), from treated domestic wastewater by intermittent media infiltration (IMI) in column was studied. The columns were filled with natural filter media (soil, soil/charcoal and zinc-modified zeolite, Zeo-Zn), and wastewater, IMI-treated wastewater and disinfected wastewater were compared. The numbers of residual FC on Swiss chard (Beta vulgaris) and in agricultural soil were determined over a 4-month period. The column using Zeo-Zn had a higher FC removal efficiency (2.98 log) than columns with other filter media and disinfection (1.87-2.57 log) due to the bactericidal properties of Zn(2+). The treatment of wastewater using Zeo-Zn and disinfection both decreased the accumulation of FC on plants and in soil to approximately 1-20 MPN/g dry matter. IMI-treated wastewater using the column with Zeo-Zn was suitable for unrestricted agricultural use, complied with Mexican regulations (as did disinfected wastewater) and had a low risk of FC contamination of plants and soil. PMID:23168639

  13. The survival of Escherichia coli, faecal coliforms and enterobacteriaceae in general in soil treated with sludge from wastewater treatment plants.

    Estrada, I B; Aller, A; Aller, F; Gómez, X; Morán, A

    2004-06-01

    We monitored the effect of the application of treated sludge on the behaviour of enterobacteriaceae (mainly faecal coliforms and especially Escherichia coli) in the soil, and studied their evolution over time after application. Three different sludges were used: two from a municipal sewage plant, one of them had been subjected to anaerobic digestion and heat drying, and the other to anaerobic digestion and mechanical dehydration, and one from a dairy waste treatment to aerobic digestion and gravity thickening. Two types of tests were carried out: type O, in the open air, with no possibility of controlling humidity or temperature; and type L, under laboratory conditions, with controlled temperature and humidity. Sludge tests were also run on unscreened soil previously treated with chemical fertilizer. After 80 days of experimentation the populations of faecal coliforms and E. coli had decreased considerably or were undetectable in assays carried out on the soil/sludge mixtures, under both open-air and laboratory conditions, but that, over the same period, in the mixtures containing chemical fertilizer (calcium ammonium nitrate) there had been a considerable increase in the micro-organism populations studied. PMID:15051081

  14. Evaluating the association between passive smoking with total antioxidant capacity and salivary lipid peroxidation levels in 12 to 15 year old adolescents

    M Mottalebnejad; M Pouramir; N. Jenabian; M Ranjbar Omrani; A Bijani; F yarmand

    2014-01-01

    Abstract:   Background and Aim:  Passive cigarette smoking or environmental tobacco smoke (ETS) exposure is a major public health concern. Children are the most vulnerable group to ETS exposure. The aim of this study was to evaluate the relationship between passive smoking and Total Antioxidant Capacity (TAC) and salivary lipid peroxidation in adolescents. Materials and Methods: In this historical cohort study sixty adolescents aged 12 to 15 years old were selected. The s...

  15. A study of lipid- and water-soluble arsenic species in liver of Northeast Arctic cod (Gadus morhua) containing high levels of total arsenic.

    Sele, Veronika; Sloth, Jens J; Julshamn, Kåre; Skov, Kasper; Amlund, Heidi

    2015-04-01

    In the present study liver samples (n=26) of Northeast Arctic cod (Gadus morhua), ranging in total arsenic concentrations from 2.1 to 240mg/kg liver wet weight (ww), were analysed for their content of total arsenic and arsenic species in the lipid-soluble and water-soluble fractions. The arsenic concentrations in the lipid fractions ranged from 1.8 to 16.4mg As/kg oil of liver, and a linear correlation (r(2)=0.80, pHPLC-ICP-MS), revealed the presence of several arsenolipids. Three major arsenic-containing hydrocarbons (C17H39AsO, C19H41AsO and C23H37AsO) and five arsenic-containing fatty acids (C17H35AsO3, C19H39AO3, C19H37AsO3, C23H37AsO3 and C24H37AsO3) were identified using HPLC coupled to quadrupole time-of-flight mass spectrometry (qTOF-MS). Arsenobetaine was the major arsenic species in the water-soluble fraction of the livers, while dimethylarsinate, arsenocholine and inorganic arsenic were minor constituents. Inorganic arsenic accounted for less than 0.1% of the total arsenic in the liver samples. PMID:25618262

  16. Effects of apple pomace proportion levels on the fermentation quality of total mixed ration silage and its digestibility, preference and ruminal fermentation in beef cows.

    Fang, Jiachen; Cao, Yang; Matsuzaki, Masatoshi; Suzuki, Hiroyuki

    2016-02-01

    Four Japanese black beef cows were used in a 4 × 4 Latin square to evaluate the fermentation quality, digestibility, ruminal fermentation and preference of total mixed ration (TMR) silages prepared with differing proportions of apple pomace (AP). Experimental treatments were the control (no AP added, CAP), 5% (low, LAP), 10% (medium, MAP) and 20% (high, HAP) of TMR dry matter (DM) as AP. All TMR silages were well preserved. Ethanol was produced in silages containing AP and the amount increased with the proportion of AP (P silages should be less than 5% of dietary DM. PMID:26278555

  17. 调味品中大肠菌群检验的不确定度评定%Uncertainty evaluation for testing of fecal coliforms in Condiment

    温建荣; 吴芸芸; 吴俐

    2015-01-01

    对调味品中大肠菌群检验的不确定度进行评定.本次实验的扩展不确定度为0.0749,此方法适合于同类产品大肠茵群检验结果不确定度的评定.%The testing uncertainty of coliform group in condiment was evaluated. The expanded uncertainty was 0.251 in this experiment. This method is suitable for uncertainty evaluation for testing of coliforms in the similar products.

  18. The effect of zinc oxide supplementation on the stability of the intestinal flora with special reference to composition of coliforms in weaned pigs.

    Katouli, M; Melin, L; Jensen-Waern, M; Wallgren, P; Möllby, R

    1999-10-01

    The effect of a dietary supplementation of zinc oxide (ZnO) on the stability of the intestinal flora and on the composition of coliforms in weaned pigs was investigated. Faecal floras were characterized by their metabolic activities and fermentative capacity (FC) using the Phene Plate generalized microplate. Coliforms were characterized by conventional enumeration and by the Phene Plate-RS plates. The latter measured FC, phenotypic diversity, persistence of each coliform strain in piglets, and similarity among the coliform populations within groups. From weaning onwards, the control pigs (n = 5) were fed a basal diet ad libitum, while experimental pigs (n = 5) were given the same food supplemented with 2500 ppm ZnO. Metabolic fingerprinting of faecal floras indicated marked differences between the composition of floras of treated and control pigs during the first 2 weeks post-weaning. The FC of faecal flora in both groups decreased as pigs aged, but it was significantly (P pigs during the first 2 weeks post-weaning. Neither the number of coliforms, nor their FC-values, differed between the groups. However, during the first 2 weeks post-weaning, there was a significant increase in both variety (P = 0.019) and diversity (P pigs compared with the ZnO treated group. Homogeneity between coliform populations of piglets was high during the suckling period, indicating the presence of many identical strains among piglets. This, however, decreased in control pigs during the first 2 weeks post-weaning. Several coliform strains that colonized the gut at the early stage of the pigs' life were found to be resident in animals of both groups. It is concluded that supplementation of ZnO to weaned pigs helped to maintain the stability of the intestinal microflora and the diversity of coliforms during the first 2 weeks post-weaning, but not later, and that ZnO supplementation to creep feed should be restricted to the first 2 weeks post-weaning in veterinary practice. PMID:10583685

  19. Estimating Bacterial Pathogen Levels in New Zealand Bulk Tank Milk.

    Marshall, J C; Soboleva, T K; Jamieson, P; French, N P

    2016-05-01

    Zoonotic bacteria such as Campylobacter, Listeria, and Shiga toxin-producing Escherichia coli have been found in bulk tank milk in many countries, and the consumption of raw milk has been implicated in outbreaks of disease in New Zealand. Fecal contamination at milking is probably the most common source of pathogenic bacteria in bulk tank milk. Raw milk was collected from 80 New Zealand dairy farms during 2011 and 2012 and tested periodically for Campylobacter, E. coli O157, Listeria monocytogenes, and Salmonella. Milk quality data such as coliform counts, total bacterial counts, and somatic cell counts also were collected. By treating the total bacterial count as a proxy for fecal contamination of milk and utilizing farm and animal level prevalence and shedding rates of each pathogen, a predictive model for the level of pathogenic bacteria in bulk tank raw milk was developed. The model utilizes a mixture distribution to combine the low level of contamination inherent in the milking process with isolated contamination events associated with significantly higher pathogen levels. By simulating the sampling and testing process, the predictive model was validated against the observed prevalence of each pathogen in the survey. The predicted prevalence was similar to the observed prevalence for E. coli O157 and Salmonella, although the predicted prevalence was higher than that observed in samples tested for Campylobacter. PMID:27296424

  20. Measurement of total IgE antibody levels in lacrimal fluid of patients suffering from atopic and non-atopic eye disorders. Evidence for local IgE production in atopic eye disorders?

    Aalders-Deenstra, V; Kok, P T; Bruynzeel, P L

    1985-01-01

    Total IgE levels in lacrimal fluid of patients suffering from different eye disorders were quantitatively measured by a modification of the paper radio immuno sorbent test (PRIST). The geometric mean values for patients with atopic conjunctivitis, patients with keratoconjunctivitis vernalis, and patients with asthma without conjunctivitis differed significantly from those for control persons and those for patients without atopic conjunctivitis. Besides lacrimal fluid IgE levels, serum IgE lev...

  1. Study on total and methyl mercury levels in human scalp hairs of lying-in women and newborns by NAA and other techniques

    Since the Second Research Co-ordinating Meeting in Malaysia, 24-28 August 1992, our research group has completed the analysis of total and methylmercury in scalp hair samples of 1179 fishermen living at a typical Hg-polluted region in Northeast China and of 27 lying-in women and their newborns in a Beijing hospital by INAA, GC(EC) and other techniques. The longitudinal Hg patterns of the lying-in women show a gradually decreasing tendency during the pregnancy period. Further, the hair Hg contents of the newborn babies are generally above or close to those of their mothers, confirming the mechanism that the methylmercury, an organic species of Hg with high toxicity, is readily able to penetrate the placental barrier and accumulate in the fetus. Thus, the mercury exposure has occurred at the early stage of pregnancy. (author)

  2. Comparison of Serum Uric Acid, Iron and Total Iron Binding Capacity (TIBC) Levels in Pre-eclamptic and Normal Pregnant Women

    2010-01-01

    Objective: The objective of our study was to compare uric acid, iron and TIBC levels in normal and preeclamptic pregnant women and determine their relations with maternal and fetal complications. Materials and methods: A case control study was conducted in 200 normal and preeclamptic pregnant women. At 32-40 weeks of pregnancy (third trimester) a blood test was taken in order to measure the uric acid, iron and TIBC and their relation with maternal and fetal complications. Results: Uric ...

  3. Attenuation of the Disruptive Effect induced by the Insecticide Fenvalerate on Total Monoamine Content and Testosterone Level in Adult Male Albino Rats Using Salvia aegyptiaca Extract

    Administration of fenvalerate (90 mg/kg) to rats resulted in a significant decrease in dopamine (OA) content in most of brain areas under investigation. Its content in pons + medulla oblongata was the most affected recording - 62.98 %, on day 7, compared to control. Furthermore, norepinephrine (NE) content gradually decreased in different brain areas showing its maximal decrease in cerebellum with percentage change -64.89% on day 7. Serotonin (5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) showed maximal significant decrease, in the cortex with percentage differences -78.33 and -72.61%, respectively. Similarly, fen valerate resulted in a gradual decrease in serum testosterone level recording its maximal effect (-46. 58 %) at the end of the experimental period. On the other hand, administration of Salvia aegyptiaca (2 g/kg) caused a significant increase in monoamine contents (DA, NE, 5-HT and 5- HlAA) in most of the brain areas under investigation, throughout the experimental period. Moreover, Salvia extract administration resulted in a significant elevation in serum testosterone level, one day after administration, recording its maximal effect (55.75%) on day 7. Animals that received the combined treatment (Salvia extract one hour after fen valerate administration) showed that monoamine contents in most of the brain areas were more or less near to the control values. Furthermore, no significant change was noticed in serum testosterone level throughout the experiment in the combined treatment. From the present study, it can be concluded that Salvia aegyptiaca extract seems to be potentially promising for attenuating the disruption that occurred in monoamine and testosterone levels. This could highly recommend Salvia aegyptiaca to be a potential herb for further studies in the future for extracting compounds of medical use

  4. Delayed accumulation of intestinal coliform bacteria enhances life span and stress resistance in Caenorhabditis elegans fed respiratory deficient E. coli

    Gomez Fernando

    2012-12-01

    Full Text Available Abstract Background Studies with the nematode model Caenorhabditis elegans have identified conserved biochemical pathways that act to modulate life span. Life span can also be influenced by the composition of the intestinal microbiome, and C. elegans life span can be dramatically influenced by its diet of Escherichia coli. Although C. elegans is typically fed the standard OP50 strain of E. coli, nematodes fed E. coli strains rendered respiratory deficient, either due to a lack coenzyme Q or the absence of ATP synthase, show significant life span extension. Here we explore the mechanisms accounting for the enhanced nematode life span in response to these diets. Results The intestinal load of E. coli was monitored by determination of worm-associated colony forming units (cfu/worm or coliform counts as a function of age. The presence of GFP-expressing E. coli in the worm intestine was also monitored by fluorescence microscopy. Worms fed the standard OP50 E. coli strain have high cfu and GFP-labeled bacteria in their guts at the L4 larval stage, and show saturated coliform counts by day five of adulthood. In contrast, nematodes fed diets of respiratory deficient E. coli lacking coenzyme Q lived significantly longer and failed to accumulate bacteria within the lumen at early ages. Animals fed bacteria deficient in complex V showed intermediate coliform numbers and were not quite as long-lived. The results indicate that respiratory deficient Q-less E. coli are effectively degraded in the early adult worm, either at the pharynx or within the intestine, and do not accumulate in the intestinal tract until day ten of adulthood. Conclusions The findings of this study suggest that the nematodes fed the respiratory deficient E. coli diet live longer because the delay in bacterial colonization of the gut subjects the worms to less stress compared to worms fed the OP50 E. coli diet. This work suggests that bacterial respiration can act as a virulence factor

  5. {sup 18}F-FDG uptake by spleen helps rapidly predict the dose level after total body irradiation in a Tibetan minipig model

    Wang, Yu Jue; Gu, Wei Wang [Southern Medical University, Department of Laboratory Animal Center, Guangzhou, Guangdong (China); Wu, Shao Jie; Guo, Kun Yuan; Chen, Chi [Southern Medical University, Department of Hematology, Zhujiang Hospital, Guangzhou, Guangdong (China); Xie, Qiang; Cai, Liang [Chinese People' s Armed Police Forces, Department of Oncology and PET/CT, Guangdong Provincial Corp Hospital, Guangzhou, Guangdong (China); Zou, Fei [Southern Medical University, School of Public Health and Tropical Medicine, Guangzhou, Guangdong (China)

    2012-09-15

    To investigate whether {sup 18}F- FDG uptake can be applied in dosimetry to facilitate the rapid and accurate evaluation of individual radiation doses after a nuclear accident. Forty-eight Tibetan minipigs were randomised into a control group (n = 3) and treatment groups (n = 45). {sup 18}F-FDG combined positron-emission tomography and computed tomography (PET/CT) were carried out before total body irradiation (TBI) and at 6, 24 and 72 h after receiving TBI doses ranging from 1 to 11 Gy. Spleen tissues and blood samples were also collected for histological examination, apoptosis and blood analysis. Mean standardised uptake values (SUVs) of the spleen showed significant differences between the experimental and the control groups. Spleen SUV at 6 h post-irradiation showed significant correlation with radiation dose; Spearman's correlation coefficient was 0.97 (P < 0.01). Histological observations showed that damage to the splenic lymphocyte became more severe with an increase in the radiation dose. Moreover, apoptosis was one of the major routes of splenic lymphocyte death, which was also confirmed by flow cytometry analysis. In the Tibetan minipig model, radiation doses have a close relationship with the {sup 18}F-FDG uptake of the spleen. This finding suggests that {sup 18}F-FDG PET/CT may be useful for the rapid detection of individual radiation doses. (orig.)

  6. 自制大肠菌群快速检测纸片的应用效果%Application Effect of Self-made Rapid Detection Paper for Coliform Bacteria

    郝生宏

    2012-01-01

    [Objective ] The aim was to explore the application effect of self-made rapid detection paper for coliform bacteria. [ Method ] The quantities of coliform bacteria in tableware and water collected from Liaoning Agricultural Vocational-technical College were determined by self-made rapid detection paper, meanwhile detection results of self-made rapid detection paper, saled paper and national standard method were compared. [Result] When the water was detected, the result of self-made rapid detection paper was consistent with the coincidence rate and the passing rate of national standard method. When the tableware was detected, the result of self-made rapid detection paper was consistent with the coincidence rate and the passing rate of saled paper. [ Conclusion ] The self-made rapid detection paper for coliform bacteria could save more manpower and material resources than national standard method, and the detection result is similar to that of saled paper, so it is suitable for food microorganism monitoring of basic level inspection department.%[目的]探究自制大肠菌群快速检测纸片的应用效果.[方法]以自制大肠菌群快速检测纸片为材料,对辽宁农业职业技术学院餐具及水质进行了监测,并比较了自制纸片、市售纸片和国标方法的检测结果.[结果]检测水质时,自制纸片与国标法的符合率、合格率相一致;检测餐具时,自制纸片与市售纸片的符合率、合格率相一致.[结论]纸片法比发酵法能大量节省人力和物力,且与市售纸片结果相似,适合在基层检验单位推广使用.

  7. Remoción de fenoles, detergentes y coliformes presentes en aguas residuales por medio de irradiación

    Jaime Moreno; Arturo Colín; Octavio Vázquez

    1992-01-01

    Muestras de aguas con distinto grado de contaminación (con tratamiento primario y biológico), fueron expuestas a diez diferentes dosis de radiación gamma de Co-60 en el intervalo de 6 a 37 kCy, a una razón de dosis de 25 kCy/h para definir en que etapa del tratamiento convencional de las aguas residuales, la radiación puede remover contaminantes químicos y biológicos. Con 6 a 10 kGy se eliminó hasta 5 órdenes de magnitud de microorganismos coliformes. No se encontró una dosis especifica para ...

  8. Branch-level measurement of total OH reactivity for constraining unknown BVOC emission during the CABINEX (Community Atmosphere-Biosphere INteractions Experiments-09 Field Campaign

    S. Kim

    2011-03-01

    Full Text Available We present OH reactivity measurements using the comparative reactivity method with a branch enclosure technique for four different tree species (red oak, white pine, beech and red maple in the UMBS PROPHET tower footprint during the Community Atmosphere Biosphere INteraction EXperiment (CABINEX field campaign in July of 2009. Proton Transfer Reaction-Mass Spectrometry (PTR-MS was sequentially used as a detector for OH reactivity and BVOC including isoprene and monoterpenes (MT, in enclosure air, so that the measurement dataset contains both measured OH reactivity and calculated OH reactivity from well-known BVOC. The results indicate that isoprene and MT, and in one case a sesquiterpene, can account for the measured OH reactivity. Significant discrepancy between measured OH reactivity and calculated OH reactivity from isoprene and MT is found for the red maple enclosure dataset but it can be reconciled by adding reactivity from emission of a sesquiterpene, α-farnesene, detected by GC-MS. This leads us to conclude that no significant unknown BVOC emission contributed to ambient OH reactivity from these trees at least during the study period. This conclusion leads us to explore the contribution from unmeasured isoprene (the dominant OH sink in this ecosystem oxidation products such as hydroxyacetone, glyoxal, methylglyoxal and C4 and C5-hydroxycarbonyl using recently published isoprene oxidation mechanisms (Mainz Isoprene Mechanism II and Leuven Isoprene Mechanism. Evaluation of conventionally unmeasured first generation oxidation products of isoprene and their possible contribution to ambient missing OH reactivity indicates that the ratio of OH reactivity from unmeasured products over OH reactivity from MVK + MACR is strongly dependent on NO concentrations. The unmeasured oxidation products can contribute ~7.2% (8.8% from LIM and 5.6% by MIM 2 when NO = 100 pptv of the isoprene contribution towards total ambient OH reactivity. This amount can

  9. Changes in Aerobic Plate and Escherichia coli-Coliform Counts and in Populations of Inoculated Foodborne Pathogens on Inshell Walnuts during Storage.

    Frelka, John C; Davidson, Gordon R; Harris, Linda J

    2016-07-01

    After harvest, inshell walnuts are dried using low-temperature forced air and are then stored in bins or silos for up to 1 year. To better understand the survival of bacteria on inshell walnuts, aerobic plate counts (APCs) and Escherichia coli?coliform counts (ECCs) were evaluated during commercial storage (10 to 12°C and 63 to 65% relative humidity) over 9 months. APCs decreased by 1.4 to 2.0 log CFU per nut during the first 5 months of storage, and ECCs decreased by 1.3 to 2.2 log CFU per nut in the first month of storage. Through the remaining 4 to 8 months of storage, APCs and ECCs remained unchanged (P > 0.05) or decreased by drying (10 to 12 h; 40°C) and simulated commercial storage (12 months at 10°C and 65% relative humidity). Populations declined by 2.86, 5.01, and 4.40 log CFU per nut for E. coli O157:H7, L. monocytogenes, and Salmonella, respectively, after drying and during the first 8 days of storage. Salmonella populations changed at a rate of -0.33 log CFU per nut per month between days 8 and 360, to final levels of 2.83 ± 0.79 log CFU per nut. E. coli and L. monocytogenes populations changed by -0.17 log CFU per nut per month and -0.26 log CFU per nut per month between days 8 and 360, respectively. For some samples, E. coli or L. monocytogenes populations were below the limit of detection by plating (0.60 log CFU per nut) by day 183 or 148, respectively; at least one of the six samples was positive at each subsequent sampling time by either plating or by enrichment. PMID:27357033

  10. Increased plasma total cholesterol and high density lipoprotein levels produced by the crude extract from the leaves of Viscum album (mistletoe).

    Ben, E E; Eno, A E; Ofem, O E; Aidem, U; Itam, E H

    2006-01-01

    The effect of an aqueous extract prepared from the leaves of Viscum album (Mistletoe) on plasma cholesterol and albumin levels in male Wistar rats was studied. Lethality studies revealed that the extract had an LD50 value of 417.0 mg/kg mice, intraperitoneally. The rats were randomly divided into seven (7) groups of 5 rats per group with one animal per metabolic cage. Group one served as the control (C1), groups two to six were treated with extract (200 mg/kg body weight orally and daily) for a maximum of ten (10) weeks, whereas, group seven (C2) received no extract treatment but was fed on normal rat chow. All the rats had free access to rat food and drinking water. The first group (C1) was sacrificed a fortnight after the commencement of the experiment, while group seven (C2) was sacrificed at the end (10th week) of the experiment. The extract-treated groups were sacrificed respectively in the order two, four, six, eight and ten week of extract administration. Whole blood was collected from these groups for analysis. Results showed significant [P control values. From the results, it is suggested that the crude aqueous extract from mistletoe leaf may be relatively safe for therapeutic use as it neither predisposes to cardiovascular risk nor adversely affects protein metabolism following prolonged period of administration. PMID:17242719

  11. 即食凉拌食品中微生物污染情况及大肠菌群限量的调查分析%Investigation of Instant Cold Foods and Microbial Contamination of Coliform Limit

    李梓娴; 速存芬; 陈磊; 刘辉光

    2015-01-01

    Objective The purpose of the microbial contamination of food ready to eat salad investigation and analysis, to develop a reliable instant cold food microbiological limits. Methods A "People's Republic of China national standards of food hygiene mi-crobiological testing methods" and the national monitoring network and other food-borne illness as a monitoring program, instant cold foods in the restrants and supermarbets of Qujing for microbial contamination were detected From May to september 2014. The main test items include coliform bacteria, yeasts, Staphylococcus aureus, Shigella, Salmonella, hemolytic streptococcus, E. coli cause diarrhea, Listeria monocytogenes, and the total number of colonies. Results 80 parts of instant salad product samples exam-ined 31 retail processing unit processing the target pathogen detection does not appear. The positive rate of coliform detection pro-cess is about 72.25%, the main contaminating bacteria. Conclusion Coliforms in instant cold dishes as the main pollution flora, for a limited countries such flora as soon as possible as soon as possible health and safety standards, recommended instant cold foods coliform limit should be less than 100 MPN/g.%目的:对即食凉拌食品的微生物污染状况进行调查分析,为即食凉拌食品制定出可靠的微生物限量标准。方法采用《中华人民共和国国家标准食品卫生微生物学检验方法》以及国家食源性疾病监测网等作为监测方案,2014年5要9月份曲靖市管辖区部分饭店超市的即食凉拌食品中微生物污染情况进行检测。主要检测项目包括大肠菌群、酵母菌、金黄色葡萄球菌、志贺菌、沙门菌、溶血性链球菌、致泻性大肠埃希菌、单增李斯特菌以及菌落总数。结果进行检查的31家零售加工单位加工生产的80份即食凉拌品样品中未出现目标致病菌的检出。检测过程中大肠菌群的阳性率约为72.25%,为主要污染菌。结论在

  12. Five-year clinical results of cervical total disc replacement compared with anterior discectomy and fusion for treatment of 2-level symptomatic degenerative disc disease: a prospective, randomized, controlled, multicenter investigational device exemption clinical trial.

    Radcliff, Kris; Coric, Domagoj; Albert, Todd

    2016-08-01

    OBJECTIVE The purpose of this study was to report the outcome of a study of 2-level cervical total disc replacement (Mobi-C) versus anterior cervical discectomy and fusion (ACDF). Although the long-term outcome of single-level disc replacement has been extensively described, there have not been previous reports of the 5-year outcome of 2-level cervical disc replacement. METHODS This study reports the 5-year results of a prospective, randomized US FDA investigational device exemption (IDE) study conducted at 24 centers in patients with 2-level, contiguous, cervical spondylosis. Clinical outcomes at up to 60 months were evaluated, including validated outcome measures, incidence of reoperation, and adverse events. The complete study data and methodology were critically reviewed by 3 independent surgeon authors without affiliation with the IDE study or financial or institutional bias toward the study sponsor. RESULTS A total of 225 patients received the Mobi-C cervical total disc replacement device and 105 patients received ACDF. The Mobi-C and ACDF follow-up rates were 90.7% and 86.7%, respectively (p = 0.39), at 60 months. There was significant improvement in all outcome scores relative to baseline at all time points. The Mobi-C patients had significantly more improvement than ACDF patients in terms of Neck Disability Index score, SF-12 Physical Component Summary, and overall satisfaction with treatment at 60 months. The reoperation rate was significantly lower with Mobi-C (4%) versus ACDF (16%). There were no significant differences in the adverse event rate between groups. CONCLUSIONS Both cervical total disc replacement and ACDF significantly improved general and disease-specific measures compared with baseline. However, there was significantly greater improvement in general and disease-specific outcome measures and a lower rate of reoperation in the 2-level disc replacement patients versus ACDF control patients. Clinical trial registration no. NCT00389597

  13. Nível sérico de IgE total em alergia respiratória: estudo em pacientes com alto risco de infecção por helmintos Total IgE level in respiratory allergy: study of patients at high risk for helminthic infection

    Décio Medeiros

    2006-08-01

    Full Text Available OBJETIVO: Eosinofilia e elevação de IgE sérica são expressões de atopia, contudo há fatores intervenientes como, por exemplo, as parasitoses intestinais. Esta pesquisa verifica a relação entre IgE sérica total, eosinófilos e IgE específica anti-áscaris em indivíduos portadores de asma e/ou rinite alérgica. MÉTODOS: Estudo do tipo transversal em adolescentes portadores de asma e/ou rinite alérgica que foram examinados quanto ao nível sérico de IgE total, de IgE anti-áscaris e de contagem dos eosinófilos sangüíneos. RESULTADOS: Foram analisados 101 pacientes com idade entre 12 e 21 anos. A mediana da IgE foi 660 UI/mL (P25-75 243,5-1500, e a dos eosinófilos foi 510 células/mm³ (P25-75 284-811. A IgE anti-áscaris foi positiva em 73% (74/101 da amostra, mas houve apenas 33,7% (34/101 de positividade ao parasitológico de fezes. Os coeficientes de correlação encontrados foram: 0,34 (p = 0,001 entre IgE total e eosinófilos, 0,52 (p OBJECTIVE: Eosinophilia and increased serum IgE levels are indicators of atopy; however, other factors can also play a key role, such as intestinal parasitic infections. This study assesses the relationship between total serum IgE, eosinophil count, and anti-Ascaris IgE in individuals with asthma and/or allergic rhinitis. METHODS: A cross-sectional study was carried out in adolescents with asthma and/or allergic rhinitis. The patients had their total serum IgE, anti-Ascaris IgE and eosinophil count measured. RESULTS: A total of 101 patients aged 12 to 21 years were assessed. Median IgE level was 660 IU/mL (P25-75 243.5-1500, and the eosinophil count corresponded to 510 cells/mm³ (P25-75 284-811. Anti-Ascaris IgE was positive in 73% (74/101 of the individuals, but parasitological stool examination yielded positive results in only 33.7% (34/101. The correlation coefficients were the following: 0.34 (p = 0.001 between total IgE level and eosinophil count, 0.52 (p < 0.001 between total IgE level

  14. Bacteriological Analysis and Hygine Level of Food Outlets within Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria.

    Ibrahim TA; Akenroye OM; Osabiya OJ

    2013-01-01

    The bacteriological quality of three major food outlets in Rufus Giwa Polytechnic, Owo, was assessed using standard bacteriological methods. Swabs of hands of food vendors, table and plates in these outlets were assessed for total bacterial count, total coliform count and total E. coli count. A total of 789 bacterial colonies were isolated from hands of food handlers, tables and plates used for eating in the outlets. Eleven genera of bacteria were isolated and identified, they were; klebsiell...

  15. Research on Precision of Trigonometric Leveling of North NTS-300 Electronic Total Station%南方NTS-300全站仪三角高程测量精度研究

    钟伟; 谢力分

    2011-01-01

    According to the principle of trigonometric leveling of enctronic total station, formula of height difference of trigonometric leveling is deduced and precision which can be reached by using the measuring method is studied.%根据全站仪三角高程测量原理,推导了全站仪三角高程测量高差的公式,并对南方NTS-300全站仪进行三角高程测量能达到的精度进行了研究分析。

  16. Colimetria de água marinha em áreas de cultivo e extrativismo de mexilhões no município de Niterói, RJ Coliform enumeration in mussel-growing waters from Niteroi city, RJ-Brazil

    A.A. Pinheiro Jr.

    2002-08-01

    Full Text Available Analisaram-se amostras de água do mar de cinco pontos da Baía de Guanabara quanto ao número mais provável (NMP de coliformes totais e de Escherichia coli usando-se o método fluorogênico, e verificaram-se o pH, a salinidade e a temperatura dos locais de colheita. As contagens de E. coli nas amostras das estações Icaraí, Boa Viagem e Santa Cruz foram, em média, acima do previsto na legislação, enquanto que nas estações Piratininga e Rio Branco, 92,9% e 100% das amostras tiveram contagens de E. coli menor que 3/100ml. Observou-se relação positiva entre coliformes totais e E. coli, enquanto que a correlação entre colimetria, pH, salinidade e temperatura da água não foi significativa. Temperatura e pH mostraram pouca variação entre as estações, sendo as médias de salinidade, coliformes totais e E. coli semelhantes em Icaraí, Boa Viagem e Santa Cruz. Piratininga e Rio Branco apresentaram médias diferentes destas e semelhantes entre si. Os resultados em Icaraí, Boa Viagem e Santa Cruz indicam provável impropriedade para o cultivo e/ou extrativismo de mexilhões destinados ao consumo, enquanto que os de Piratininga e Rio Branco indicam provável propriedade para a instalação de cultivo de mexilhões ou mesmo para depuração natural de mexilhões cultivados em águas contaminadas.Water samples from five different sites on Guanabara Bay (Icaraí, Boa Viagem, Santa Cruz, Piratininga, and Rio Branco were analyzed for estimated numbers of coliform and Escherichiacoli. Water pH, salt concentration, and temperature were measuredin the sampling place. E.coli countings for samples collected in Icaraí, Boa Viagem, and Santa Cruz were usually higher than the values legallyaccepted by the Brazilian Ministry of Agriculture. In Piratininga and Rio Branco, respectively, 92.3% and 100% of the sampleshad countings of lessthan 3 E.coli/100ml. A significant correlation was found between total coliforms and E. coli countings. The average

  17. 粪大肠菌群检测方法研究进展%Research Progress on Detection Methods of Fecal Coliform

    史建

    2012-01-01

    The fecal coliform is indicator bacteria of faecal contamination of water bodies. To describe the advantages, disadvantages and the main application examples of some detection methods for fecal coliform, including multiple tube fermentation method,membrane tilter,paper strip method, enzyme substrate EC technique and molecular biolo gy method.%粪大肠菌群是水体粪便污染的指示菌。详细介绍了多管发酵法、滤膜法、纸片法、酶底物法及分子生物学方法检测粪大肠菌群的优缺点及主要应用实例。

  18. COLIFORMES TERMOTOLERANTES EN AGUAS DE LAS POBLACIONES COSTERAS Y PALAFÍTICAS DE LA CIÉNAGA GRANDE DE SANTA MARTA, COLOMBIA

    Gomez Martha

    2008-12-01

    Full Text Available La Ciénaga Grande de Santa Marta es el sistema lagunar costero más grande e importante de Colombia y el más extenso del Caribe. En su territorio se alojan siete poblaciones carentes de un sistema de saneamiento básico adecuado, que vierten sus desechos sin ningún tipo de tratamiento directamente a la ciénaga. Teniendo en cuenta la importancia ambiental de este cuerpo de agua y con el objeto de conocer su calidad sanitaria se determinaron las concentraciones de Coliformes termotolerantes en aguas adyacentes de dos poblaciones costeras (Tasajera e isla del Rosario y tres palafiticas (Buenavista, Nueva Venecia y Trojas de Cataca durante el periodo 2004-2007. Adicionalmente, se realizaron mediciones de pH, temperatura, salinidad y oxígeno disuelto en cada sitio de muestreo. Las mayores concentraciones de Coliformes se encontraron en el palafito Trojas de Cataca, en la época seca mayor de cada año (7.800-9.000 NMP/ 100 mL y los menores niveles en la estación Buenavista (2-1.100 NMP/100mL. El patrón de comportamiento de las densidades de Coliformes en las poblaciones costeras fue similar en el tiempo y no se observó ninguna relación directa entre las variables fisicoquímicas y el grupo Coliformes. Dado lo observado, se considera importante vigilar la calidad del agua en el estuario, teniendo en cuenta que constituye una fuente potencial de riesgo para el desarrollo de las actividades económicas de la zona y para la salud de los pobladores.

  19. Coliform and helminth eggs removal in a combined UASB reactor-baffled pond system in Brazil: performance evaluation and mathematical modelling.

    von Sperling, M; Chernicharo, C A L; Soares, A M E; Zerbini, A M

    2002-01-01

    The paper presents the monitoring results of a pilot UASB reactor followed by a baffled polishing pond treating domestic sewage in Brazil. Longitudinal profiles of E coli and helminth eggs along the baffled pond have been undertaken. The experimental results have been compared with von Sperling's model for coliform removal and Ayres' model for helminth eggs removal, and the fitting was considered satisfactory in both cases. The distribution of the helminth species along the system is also presented. PMID:12188551

  20. Circulating levels of interleukin-6, vascular endothelial growth factor, YKL-40, matrix metalloproteinase-3, and total aggrecan in spondyloarthritis patients during 3 years of treatment with TNFα inhibitors

    Pedersen, Susanne Juhl; Hetland, Merete Lund; Sørensen, Inge Juul; Østergaard, Mikkel; Nielsen, Hans Jørgen; Johansen, Julia Sidenius

    2010-01-01

    The objectives of the study were to investigate short and long-term changes and relations to treatment response of plasma interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), YKL-40, matrix metalloproteinase-3 (MMP-3), and total aggrecan in patients with spondyloarthritis (SpA) treated...... with tumor necrosis factor-alpha (TNFα) inhibitors and to compare with levels in healthy subjects. Biomarkers were measured in an observational cohort of 49 SpA patients (ankylosing spondylitis, n=32, and psoriatic arthritis, n=17) initiating TNFα inhibitor therapy (infliximab, n=38; etanercept, n=8...

  1. Circulating levels of interleukin-6, vascular endothelial growth factor, YKL-40, matrix metalloproteinase-3, and total aggrecan in spondyloarthritis patients during 3 years of treatment with TNF alpha inhibitors

    Pedersen, S.J.; Hetland, M.L.; Sørensen, Inge Juul; Østergaard, Mikkel; Nielsen, H.J.; Johansen, J.S.

    2010-01-01

    The objectives of the study were to investigate short and long-term changes and relations to treatment response of plasma interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), YKL-40, matrix metalloproteinase-3 (MMP-3), and total aggrecan in patients with spondyloarthritis (SpA) treated...... with tumor necrosis factor-alpha (TNF alpha) inhibitors and to compare with levels in healthy subjects. Biomarkers were measured in an observational cohort of 49 SpA patients (ankylosing spondylitis, n = 32, and psoriatic arthritis, n = 17) initiating TNF alpha inhibitor therapy (infliximab, n = 38...

  2. Circulating levels of interleukin-6, vascular endothelial growth factor, YKL-40, matrix metalloproteinase-3, and total aggrecan in spondyloarthritis patients during 3 years of treatment with TNFα inhibitors

    Sørensen, Inge Juul; Ostergaard, Mikkel; Nielsen, Hans Jørgen; Pedersen, Susanne Juhl; Hetland, Merete Lund; Østergaard, Mikkel; Johansen, Julia Sidenius

    2010-01-01

    The objectives of the study were to investigate short and long-term changes and relations to treatment response of plasma interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), YKL-40, matrix metalloproteinase-3 (MMP-3), and total aggrecan in patients with spondyloarthritis (SpA) treated...... with tumor necrosis factor-alpha (TNFa) inhibitors and to compare with levels in healthy subjects. Biomarkers were measured in an observational cohort of 49 SpA patients (ankylosing spondylitis, n=32, and psoriatic arthritis, n=17) initiating TNFa inhibitor therapy (infliximab, n=38; etanercept, n=8...

  3. Avaliação de ensaios analíticos para detecção de coliformes fecais em queijo Minas

    Pereira M.L.

    1999-01-01

    Full Text Available Foram submetidas à pesquisa de coliformes fecais, utilizando-se a técnica do número mais provável (NMP/g, 168 amostras de variedades de queijo Minas (20 frescal, 48 canastra e 100 padronizado coletadas em Belo Horizonte. Para a comparação de diferentes ensaios em temperatura elevada, utilizou-se o caldo EC isoladamente, e caldos EC e triptofano em paralelo. Visando à pesquisa de indol foi realizado ensaio para confirmação de produção de beta-D-glucuronidase e indol em caldo fluorocult lauril sulfato. Os resultados demonstraram não haver diferença estatística significativa entre as três metodologias utilizadas para a pesquisa de coliformes fecais, considerando os índices de aceitação definidos pelos padrões legais de inspeção de queijo Minas. A facilidade de execução do ensaio da beta-D-glucuronidase em caldo fluorocult lauril sulfato, associada à confiabilidade dos resultados e demanda de tempo (redução de 96 para até 48h, permitem sugeri-lo como método de escolha para enumeração de coliformes em queijo Minas.

  4. The association between bedding material and the bacterial counts of Staphylococcus aureus, Streptococcus uberis and coliform bacteria on teat skin and in teat canals in lactating dairy cattle.

    Paduch, Jan-Hendrik; Mohr, Elmar; Krömker, Volker

    2013-05-01

    Several mastitis-causing pathogens are able to colonize the bovine teat canal. The objective of this study was to investigate the association between the treatment of sawdust bedding with a commercial alkaline conditioner and the bacterial counts on teat skin and in the teat canal. The study used a crossover design. Ten lactating Holstein cows that were free of udder infections and mastitis were included in the study. The animals were bedded on either untreated sawdust or sawdust that had been treated with a hydrated lime-based conditioner. Once a day, fresh bedding material was added. After 3 weeks, the bedding material was removed from the cubicles, fresh bedding material was provided, and the cows were rotated between the two bedding material groups. Teat skin and teat canals were sampled using the wet and dry swab technique after weeks 1, 2, 3, 4, 5 and 6. Staphylococcus aureus, Streptococcus uberis, Escherichia coli and other coliform bacteria were detected in the resulting agar plate cultures. The treatment of the bedding material was associated with the teat skin bacterial counts of Str. uberis, Esch. coli and other coliform bacteria. An association was also found between the bedding material and the teat canal bacterial counts of coliform bacteria other than Esch. coli. For Staph. aureus, no associations with the bedding material were found. In general, the addition of a hydrated lime-based conditioner to sawdust reduces the population sizes of environmental pathogens on teat skin and in teat canals. PMID:23445624

  5. Development and validation of a FISH-based method for the detection and quantification of E. coli and coliform bacteria in water samples.

    Hügler, Michael; Böckle, Karin; Eberhagen, Ingrid; Thelen, Karin; Beimfohr, Claudia; Hambsch, Beate

    2011-01-01

    Monitoring of microbiological contaminants in water supplies requires fast and sensitive methods for the specific detection of indicator organisms or pathogens. We developed a protocol for the simultaneous detection of E. coli and coliform bacteria based on the Fluorescence in situ Hybridization (FISH) technology. This protocol consists of two approaches. The first allows the direct detection of single E. coli and coliform bacterial cells on the filter membranes. The second approach includes incubation of the filter membranes on a nutrient agar plate and subsequent detection of the grown micro-colonies. Both approaches were validated using drinking water samples spiked with pure cultures and naturally contaminated water samples. The effects of heat, chlorine and UV disinfection were also investigated. The micro-colony approach yielded very good results for all samples and conditions tested, and thus can be thoroughly recommended for usage as an alternative method to detect E. coli and coliform bacteria in water samples. However, during this study, some limitations became visible for the single cell approach. The method cannot be applied for water samples which have been disinfected by UV irradiation. In addition, our results indicated that green fluorescent dyes are not suitable to be used with chlorine disinfected samples. PMID:22179640

  6. Determination of IgG and IgM levels in sera of newborn calves until the 10th day of life by ELISA and description of their correlation to total plasma protein concentration and GGT activity.

    Bender, Petra; Bostedt, Hartwig

    2009-02-01

    The aim of this study was the determination of IgG and IgM concentrations in sera of 15 vital and healthy calves from the day of birth to the 10th day of life using two ELISAs exclusively developed for this purpose. We investigated if and to which extent the sera profiles were correlated with antibody levels in the colostral milk administered, with GGT activity and with total plasma protein content. Due to the assays' high sensitivity, traces of IgG and IgM in calf sera could be determined prior to the first uptake of the foremilk. Throughout the colostrum administration period until the 12th living hour, IgG and IgM levels remarkably increased (P colostrum administered was highly significant (P colostrum containing maximum or minimum immunoglobulin concentrations does not necessarily result in the respective sera immunoglobulin concentrations. From these findings, as well as from the fact that numerous subjects displayed their highest IgG and IgM sera concentrations well after the gut closure, we conclude that individually diverse resorption patterns are in place which cannot be characterized by immunoglobulin measurements only. The determination of the total plasma protein content or GGT activity in calf serum at 24 h post natum only give a rough idea about the actual immunoglobulin supply of the calves, since for the individual subject no conclusion could be drawn to the extent of immunoglobulin concentrations. PMID:19244827

  7. COMPORTAMIENTO DE COLIFORMES FECALES COMO INDICADORES BACTERIANOS DE CONTAMINACIÓN FECAL EN DIFERENTES MEZCLAS DE BIOSÓLIDO Y ESTÉRILES UTILIZADOS PARA LA RESTAURACIÓN ECOLÓGICA DE LA CANTERA SORATAMA, BOGOTÁ

    J. Fuccz-Gamboa

    2007-06-01

    Full Text Available El biosólido de la planta de tratamiento de aguas residuales “El Salitre”, contiene una importante concentración de materia orgánica y nutrientes, los cuales pueden ser utilizados como enmienda orgánica en la agricultura, reforestación y recuperación de terrenos disturbados por el hombre. Su aplicación se encuentra condicionada a la concentración de microorganismos patógenos presentes, ya que generan un riesgo sanitario para las personas que lo manipulan o entran en contacto directo con él. El objetivo de este estudio fue evaluar el comportamiento de los coliformes fecales, como indicadores bacterianos de contaminación fecal en mezclas de biosólido y estériles en diferentes proporciones utilizadas para la restauración ecológica de la cantera de “Soratama” en un período de 60 días. Se realizaron tres tratamientos (T1 8:1; T2 4:1 y T3 2:1, v/v de estériles-biosólido con tres réplicas cada uno y un control negativo, únicamente con estériles. Se tomaron un total de 60 muestras y se analizaron de acuerdo con el protocolo de la EPA/625/R92/013(1999, técnica de filtración por membrana. La concentración final de coliformes fecales fue de 2.3 x 103UFC/g de PS para T1, 1.6 x 102 UFC/g de PS para T2 y 1.2 x 101 UFC/g de PS para T3; con un porcentaje de reducción de 38,1, 53,8 y 78,5% respectivamente. La radiación solar, la temperatura ambiente, la humedad y la precipitación influyeron significativamente en la reducción de la concentración de colifor- mes fecales.

  8. Níveis de proteína bruta em dietas para bovinos de corte: consumo e digestibilidades total e parcial dos nutrientes Crude protein levels in diets of beef cattle: intake and apparent total tract, intestinal, and ruminal digestibilities of nutrients

    Maria Andréa Borges Cavalcante

    2005-12-01

    Full Text Available Avaliaram-se os consumos e as digestibilidades total e parcial dos nutrientes em novilhos Holandês x Zebu recebendo dietas contendo quatro níveis de proteína bruta (10,5; 12; 13,5 e 15% de PB na matéria seca, constituídas de 65% de feno de capim-tifton 85 e 35% de concentrado. Foram utilizados quatro animais castrados, fistulados no rúmen e abomaso, com peso vivo médio inicial de 487,3 kg, distribuídos em um quadrado latino 4 x 4. Cada período experimental teve duração de 20 dias - 10 para adaptação às dietas, seis para coletas de amostras de fezes e digestas de abomaso, um para a coleta de líquido ruminal, um para a coleta total de urina, em 24 horas, e dois para a coleta de conteúdo ruminal. Para determinação da excreção fecal, utilizou-se o óxido crômico como indicador. Os consumos de matéria seca (MS, matéria orgânica (MO, fibra em detergente neutro (FDN e nutrientes digestíveis totais (NDT não foram influenciados pelos níveis de proteína bruta (PB das dietas, mas os consumos de PB aumentaram e os de extrato etéreo (EE e carboidratos não-fibrosos (CNF reduziram com o incremento de PB das dietas. As digestibilidades aparentes totais da MS, MO e PB aumentaram linearmente com a concentração protéica das dietas. As digestibilidades aparentes ruminal e intestinal dos nutrientes não foram afetadas pelos níveis de PB das dietas, com exceção à digestibilidade intestinal da PB, que aumentou 2,77 unidades para cada percentual de PB acrescentado à dieta.The objective of this trial was to study the effects of different dietary levels of crude protein (CP on intake and apparent total tract, intestinal, and ruminal digestibilities of nutrients in Holstein x Zebu steers. The diets contained [dry matter (DM basis]: 10.5, 12, 13.5, or 15% of CP and a forage (Tifton 85:concentrate ratio of 65:35. Four castrated animals averaging 487.3 kg of body weight at the beginning of the trial and fitted with both abomasum and

  9. Levels of direct-acting mutagens, total N-nitroso compounds in nitrosated fermented fish products, consumed in a high-risk area for gastric cancer in southern China.

    Chen, C S; Pignatelli, B; Malaveille, C; Bouvier, G; Shuker, D; Hautefeuille, A; Zhang, R F; Bartsch, H

    1992-02-01

    A high gastric cancer mortality in Fujian province (Peoples Republic of China) has been associated with the consumption of certain salted fermented fish products such as fish sauce (FS). We have investigated the levels and nature of N-nitroso compounds (NOC) and genotoxins present, before and after nitrosation, in 49 FS samples collected from villages in this high-risk area, pooled into six samples. The concentrations of total NOC before nitrosation ranged from 0.2 to 16 mumoles/l, and after nitrosation at pH 2 and pH 7, they rose by up to 4800- and 100-fold, respectively. In nitrosated samples, 40-50% of total NOC was not extractable into organic solvents; volatile N nitrosamines accounted for 1-2% and N-nitrosamino acids for 8-16% of total NOC. None of the FS samples exhibited genotoxic activity, but after nitrosation all were weakly active in the SOS chromotest. The highest SOS-inducing potency was observed with nitrosated ethyl acetate extracts of most samples. The formation of methylating agents was measured by incubation of nitrosated FS with DNA and subsequent analysis of 7-methylguanine adduct. 2 of the 6 nitrosated FS samples caused a slight increase in DNA methylation. 1 pooled home-made FS sample (the only one tested) contained tumour promoter-like substances, as measured by expression of certain EBV genes in Raji cells. HPLC fractionation of ethyl acetate extracts of FS samples allowed identification of three UV-absorbing peaks that, upon nitrosation, produced direct-acting genotoxins. This genotoxicity was partly ascribed to the formation of nitrite-derived arene diazonium cations that were characterized by a coupling reaction with N-ethyl-1-naphthylamine and thin-layer chromatography. PMID:1370720

  10. Níveis de mercúrio total em peixes de água doce de pisciculturas paulistas Mercury levels in freshwater fishes from piscicultures estabilished in São Paulo State

    Marcelo A. Morgano

    2005-06-01

    Full Text Available Avaliou-se os níveis de mercúrio total em amostras de peixes de água doce, procedentes de pesqueiros e pisciculturas de 39 regiões do estado de São Paulo. As espécies de peixes estudadas foram: pacu (Piaractus mesopotamicus, tilápia (Oreochromis niloticus, piauçu (Leoporinum sp, matrinxã (Brycon cephalus, tambaqui (Colossoma macropomum e carpa (Cyprinus carpis. Foram avaliadas três metodologias de digestão de amostras para a determinação de mercúrio total, variando-se o volume de mistura sulfonítrica utilizada e a concentração do permanganato de potássio, sendo que o uso de 10mL de mistura sulfonítrica e solução de permanganato de potássio a 7% (m/v na digestão da amostra foi a que forneceu as recuperações mais altas (96%, precisão de 1% e limite de quantificação de 0,3µg/kg. Após a validação do método, foram realizadas as determinações de teor de mercúrio total em 293 amostras de pescado, usando a técnica de espectrometria de emissão com fonte de plasma acoplado em gerador de hidretos. Os níveis médios de mercúrio encontrados variaram entre: tambaqui (0,0003-0,012mg/kg, carpa (0,063mg/kg, matrinxã (0,0003-0,074mg/kg, pacu (0,0003-0,078mg/kg, piauçu (0,0003-0,183mg/kg e tilápia (0,0003-0,217mg/kg. Os resultados mostraram que nenhuma das amostras procedentes das diferentes regiões e diferentes pesque-pagues, apresentaram níveis de mercúrio total acima do permitido pela legislação brasileira, que é de 0,5mg/kg para pescado não-predador.In this work, the levels of mercury were evaluated in samples of freshwater fish, coming from fishing-grounds and piscicultures of 39 regions of São Paulo State. The species of fish studied were: pacu (Piaractus mesopotamicus, tilápia (Oreochromis niloticus, piauçu (Leoporinum sp, matrinxã (Brycon cephalus, tambaqui (Colossoma macropomum and carpa (Cyprinus carpis. Three digestion methodologies of sample were evaluated for the mercury determination, with

  11. Total Logistic Plant Solutions

    Dusan Dorcak

    2016-02-01

    Full Text Available The Total Logistics Plant Solutions, plant logistics system - TLPS, based on the philosophy of advanced control processes enables complex coordination of business processes and flows and the management and scheduling of production in the appropriate production plans and planning periods. Main attributes of TLPS is to create a comprehensive, multi-level, enterprise logistics information system, with a certain degree of intelligence, which accepts the latest science and research results in the field of production technology and logistics. Logistic model of company understands as a system of mutually transforming flows of materials, energy, information, finance, which is realized by chain activities and operations

  12. Analysis of time-series of total and plant water stress levels using a dual-source energy balance model over agricultural crops and medium to low resolution thermal infra red remote sensing data

    Boulet, Gilles; Mougenot, Bernard; Bahir, Malik; Fanise, Pascal; Saadi, Sameh; Simonneaux, Vincent; Chebbi, Wafa; Kassouk, Zeineb; Oualid, Toufik; Olioso, Albert; Lagouarde, Jean-Pierre; Le Dantec, Valérie; Rivalland, Vincent; Zribi, Mehrez; Lili-Chabaane, Zohra

    2015-04-01

    Detecting, monitoring and mapping plant water stress with remote sensing data is a crucial component of modern agricultural water management, especially in areas with scarce water resources such as the south and the eastern parts of the Mediterranean region. Developing efficient operational methods dedicated to those three actions is thus necessary to design observing systems for areas with a mixture of irrigated, rainfed and deficit irrigation agriculture. Those systems can assist managers in tasks such as early warning of drought, real time irrigated area mapping etc. A way to quantify plant and total water stress levels is to exploit the available surface temperature data from remote sensing as a signature of the surface energy balance, including the latent heat flux. Remotely sensed energy balance models enable to estimate evapotranspiration and the water status of continental surfaces. Two-source models, such as TSEB (Norman et al., 1995) allow deriving a rough estimate of the water stress of the vegetation instead of that of a soil-vegetation composite. For the latter, a realistic underlying assumption enables to invert two unknowns (evaporation and transpiration) from a single piece of information. This assumption states that, in most cases, vegetation is unstressed, and that if vegetation is stressed, evaporation is negligible. In the latter case, if vegetation stress is not properly accounted for, the resulting evaporation will decrease to unrealistic levels (negative fluxes) in order to maintain the same total surface temperature. Actual and potential transpiration rates are combined to derive an index of plant water stress applicable to low resolution data. Here, we evaluate time series of plant water stress indices in the Kairouan area in Central Tunisia in the last few years by comparing them with 1- maps of the irrigation sectors as well as rainfall data and 2- turbulent heat flux measurements obtained at low resolution (scintillometer, eddy

  13. Relation between chronic periodontal disease and plasmatic levels of triglycerides, total cholesterol and fractions Relação entre doença periodontal crônica e os níveis plasmáticos de triglicérides, colesterol total e frações

    Ana Cristina Posch Machado

    2005-12-01

    Full Text Available Many people in the world are affected by hyperlipidemia, which is a known risk factor for atherosclerotic disease. On the other hand, periodontitis, a prevalent oral disease, has been connected to several systemic health changes, including an altered lipid metabolism. Transient and recurrent bacteremias, which may be caused by periodontal infection, induce an intense local and systemic inflammatory response, leading to changes in the whole body. The aim of the present study was to verify the relationship between severe and moderate periodontal disease and blood lipid levels. Sixty individuals seen at the clinics of the University of Taubaté, São Paulo, over 20 years old, were divided into two groups, with and without periodontitis, and paired according to sex and age. Their levels of total cholesterol, tryglicerides and fractions were determined. Variables related to high cholesterol levels, including age, sex and body mass index, were evaluated. The values recommended by the Brazilian Society of Cardiology were considered to classify lipidemia. The results showed that mean levels of cholesterol (192.1 mg/dl ± 40.9 and triglycerides (153.5 mg/dl ± 105.6 in individuals with periodontitis were higher than, but not statistically different from, those of individuals without periodontitis (186.1 mg/dl ± 35.4 and 117.5 mg/dl ± 68, respectively. Therefore, this study has demonstrated that there is no significant relationship between periodontal disease, regardless of its intensity, and blood lipid levels in the studied population.A doença periodontal crônica, bastante prevalente na população adulta, tem sido relacionada com diversas alterações sistêmicas, entre elas as dislipidemias, que são fatores de risco conhecidos para a aterosclerose. Bacteremias transitórias e recorrentes, que podem ser causadas pela infecção periodontal, levam a uma intensa resposta inflamatória local e sistêmica, promovendo modificações ao longo do corpo

  14. High levels of total ammonia nitrogen as NH4+ are stressful and harmful to the growth of Nile tilapia juveniles - doi: 10.4025/actascibiolsci.v35i4.17291

    Francisco Jackes Rodrigues da Silva

    2013-07-01

    Full Text Available This study determined whether high levels of total ammonia nitrogen (TAN as NH4+ are harmful to the growth performance of Nile tilapia juveniles. Fingerlings (0.31 ± 0.04 g were assigned to 30 polyethylene 100-L tanks in a roofed room for 12 rearing weeks. There were increasing levels of TAN by increased NH4Cl application rates (0.0; 0.25 and 0.50 g tank-1 week-1 at three conditions of water pH (acidic, 6.2 ± 0.5; neutral, 7.2 ± 0.8 and alkaline, 8.8 ± 0.3. The application of HCl to acidic tanks caused 100% of TAN to be converted into NH4+. The poorest growth performance results were observed for the alkaline tanks subjected to the highest application of NH4Cl. In acidic tanks, fish survival has dropped in those tanks under the highest application rate of NH4Cl. Tilapia growth was lower in neutral tanks when the NH4Cl application rate increased to 0.50 g tank-1 week-1. It was concluded that waterborne ionized ammonia (NH4+ is indirectly toxic to tilapia due to the harmful metabolites derived from it, such as nitrite and chloramines as well as due to water acidification.

  15. Environmental monitoring of bacterial contamination and antibiotic resistance patterns of the fecal coliforms isolated from Cauvery River, a major drinking water source in Karnataka, India.

    Skariyachan, Sinosh; Mahajanakatti, Arpitha Badarinath; Grandhi, Nisha Jayaprakash; Prasanna, Akshatha; Sen, Ballari; Sharma, Narasimha; Vasist, Kiran S; Narayanappa, Rajeswari

    2015-05-01

    The present study focuses prudent elucidation of microbial pollution and antibiotic sensitivity profiling of the fecal coliforms isolated from River Cauvery, a major drinking water source in Karnataka, India. Water samples were collected from ten hotspots during the year 2011-2012. The physiochemical characteristics and microbial count of water samples collected from most of the hotspots exhibited greater biological oxygen demand and bacterial count especially coliforms in comparison with control samples (p ≤ 0.01). The antibiotic sensitivity testing was performed using 48 antibiotics against the bacterial isolates by disk-diffusion assay. The current study showed that out of 848 bacterial isolates, 93.51% (n = 793) of the isolates were found to be multidrug-resistant to most of the current generation antibiotics. Among the major isolates, 96.46% (n = 273) of the isolates were found to be multidrug-resistant to 30 antibiotics and they were identified to be Escherichia coli by 16S rDNA gene sequencing. Similarly, 93.85% (n = 107), 94.49% (n = 103), and 90.22% (n = 157) of the isolates exhibited multiple drug resistance to 32, 40, and 37 antibiotics, and they were identified to be Enterobacter cloacae, Pseudomonas trivialis, and Shigella sonnei, respectively. The molecular studies suggested the prevalence of bla TEM genes in all the four isolates and dhfr gene in Escherichia coli and Sh. sonnei. Analogously, most of the other Gram-negative bacteria were found to be multidrug-resistant and the Gram-positive bacteria, Staphylococcus spp. isolated from the water samples were found to be methicillin and vancomycin-resistant Staphylococcus aureus. This is probably the first study elucidating the bacterial pollution and antibiotic sensitivity profiling of fecal coliforms isolated from River Cauvery, Karnataka, India. PMID:25896199

  16. Concentração plasmática de colesterol total e lipoproteína de alta densidade em novilhas mestiças doadoras de embriões tratadas com somatotropina bovina recombinante Total plasma cholesterol and high-density lipoprotein levels in crossbred heifer embryo donors treated with bovine recombinant somatotropin

    Á.M. Borges

    2001-10-01

    Full Text Available O objetivo do experimento foi o de estudar as concentrações plasmáticas de colesterol total e lipoproteína de alta densidade (HDL em novilhas mestiças tratadas com somatotropina bovina recombinante (rbST. Coletas de sangue foram feitas durante dois ciclos estrais, normal e superovulado, em 26 fêmeas distribuídas em dois tratamentos: T1 = aplicação de 500mg de rbST no terceiro dia do ciclo estral utilizado para a superovulação e T2 = controle. Análises dos metabólitos sangüíneos foram feitas utilizando-se o método enzimático, cujas concentrações médias plasmáticas de colesterol total e de HDL durante o ciclo estral normal não foram diferentes (P>0,05 entre os dois tratamentos: 87,9 e 25,8mg/dl e 85,9 e 26,7mg/dl para T1 e T2, respectivamente. O ciclo estral utilizado para a superovulação foi dividido em três períodos: P1 = do estro à inseminação artificial (0 ao15º dia, P2 = da inseminação artificial até a coleta de embriões (15º ao 21º dia e P3 = da coleta até o final do período experimental (21º ao 27º dia. As concentrações plasmáticas de colesterol total e HDL no P1 não diferiram entre os tratamentos (P>0,05. Em P2 e P3 houve diferença nas concentrações de HDL e colesterol total entre os dois tratamentos: 29,0 e 88,5mg/dl (T1 e 27,1 e 81,8mg/dl (T2 no P2; e 30,4 e 88,0mg/dl (T1 e 26,6 e 80,5mg/dl (T2 no P3, respectivamente (PThe objective of the experiment was to study the total cholesterol and high-density lipoprotein (HDL levels in crossbred heifers treated with bovine recombinant somatotropina (rbST. Blood samples were collected for two estrous cycles, normal and superovulated, from 26 animals randomly distributed into two treatments: T1 - injected with 500mg rbST on day 3 of estrous cycle and T2 - control. The lipidic metabolite levels were determined by an enzymatic method, and plasma levels of total cholesterol and HDL in normal estrous cycle did not differ (P>0.05 between treatments: 87

  17. Estimation of benchmark dose as the threshold levels of urinary cadmium, based on excretion of total protein, β 2-microglobulin, and N-acetyl-β-D-glucosaminidase in cadmium nonpolluted regions in Japan

    Previously, we investigated the association between urinary cadmium (Cd) concentration and indicators of renal dysfunction, including total protein, β 2-microglobulin (β 2-MG), and N-acetyl-β-D-glucosaminidase (NAG). In 2778 inhabitants ≥50 years of age (1114 men, 1664 women) in three different Cd nonpolluted areas in Japan, we showed that a dose-response relationship existed between renal effects and Cd exposure in the general environment without any known Cd pollution. However, we could not estimate the threshold levels of urinary Cd at that time. In the present study, we estimated the threshold levels of urinary Cd as the benchmark dose low (BMDL) using the benchmark dose (BMD) approach. Urinary Cd excretion was divided into 10 categories, and an abnormality rate was calculated for each. Cut-off values for urinary substances were defined as corresponding to the 84% and 95% upper limit values of the target population who have not smoked. Then we calculated the BMD and BMDL using a log-logistic model. The values of BMD and BMDL for all urinary substances could be calculated. The BMDL for the 84% cut-off value of β 2-MG, setting an abnormal value at 5%, was 2.4 μg/g creatinine (cr) in men and 3.3 μg/g cr in women. In conclusion, the present study demonstrated that the threshold level of urinary Cd could be estimated in people living in the general environment without any known Cd-pollution in Japan, and the value was inferred to be almost the same as that in Belgium, Sweden, and China

  18. Early postoperative albumin level following total knee arthroplasty is associated with acute kidney injury: A retrospective analysis of 1309 consecutive patients based on kidney disease improving global outcomes criteria.

    Kim, Ha-Jung; Koh, Won-Uk; Kim, Sae-Gyeol; Park, Hyeok-Seong; Song, Jun-Gol; Ro, Young-Jin; Yang, Hong-Seuk

    2016-08-01

    Hypoalbuminemia has been reported to be an independent risk factor for acute kidney injury (AKI). However, little is known about the relationship between the albumin level and the incidence of AKI in patients undergoing total knee arthroplasty (TKA). The aim of our study was to assess incidence and risk factors for AKI and to evaluate the relationship between albumin level and AKI following TKA.The study included a retrospective review of medical records of 1309 consecutive patients who underwent TKA between January 2008 and December 2014. The patients were divided into 2 groups according to the lowest serum albumin level within 2 postoperative days (POD2_alb level < 3.0 g/dL vs ≥3.0 g/dL). Multivariate logistic regression analysis was used to assess risk factors for AKI. A comparison of incidence of AKI, hospital stay, and overall mortality in the 2 groups was performed using propensity score analysis.Of 1309 patients, 57 (4.4%) developed AKI based on Kidney Disease Improving Global Outcomes criteria. Factors associated with AKI included age (odds ratio [OR] 1.05; 95% confidence interval [CI] 1.01-1.09; P = 0.030), diabetes (OR 3.12; 95% CI 1.65-5.89; P < 0.001), uric acid (OR 1.51; 95% CI 1.26-1.82; P < 0.001), beta blocker use (OR 2.65; 95% CI 1.48-4.73; P = 0.001), diuretics (OR 16.42; 95% CI 3.08-87.68; P = 0.001), and POD2_alb level < 3.0 g/dL (OR 1.92; 95% CI 1.09-3.37; P = 0.023). After propensity score analysis, POD2_alb level<3.0 g/dL was associated with AKI occurrence (OR 1.82; 95% CI 1.03-3.24, P = 0.041) and longer hospital stay (P = 0.001).In this study, we demonstrated that POD2_alb level<3.0 g/dL was an independent risk factor for AKI and lengthened hospital stay in patients undergoing TKA. PMID:27495094

  19. ONPG培养基快速测定食品中大肠菌群的研究%Rapid evaluation of coliforms from food samples in ONPG medium

    李建煌; 姚蕾; 李玉优; 李焕仪; 何亮辉; 姜春宝; 曹维强

    2015-01-01

    ABSTRACT:Objective To explore the application of o-nitrophenyl-β-D-galactopyranoside (ONPG) medium in the rapid evaluation of coliforms from food samples.MethodsThe detection results of Escherichia coliat different time in ONPG medium and lauryl sulfate tryptose (LST) were compared and analyzed byχ2-test analysis. The results of coliforms form food samples in ONPG medium and LST were also compared, and analyzed by Wilcoxon analysis.Results There was no significant difference between the results ofE. coli in ONPG medium (in 18 h) and LST (in 48 h) (P>0.05). There was also no significant difference between the results of coliforms from food samples in ONPG medium (in 18 h) and LST (in 48 h) (P>0.05).Conclusion ONPG medium can be used for rapid evaluation of coliforms from food samples.%目的:探讨邻硝基苯-β-D-吡喃半乳糖苷(o-Nitrophenyl-β-D-Galactopyranoside, ONPG)培养基在快速测定食品中大肠菌群的应用。方法比较ONPG培养基和月桂基硫酸盐胰蛋白胨(lauryl sulfate tryptose, LST)肉汤测定大肠埃希氏菌不同时段的结果,使用χ2-test分析;比较ONPG培养基和LST肉汤测定食品中大肠菌群的结果,使用Wilcoxon配对法分析。结果测定大肠埃希氏菌时ONPG培养基18 h结果与LST肉汤48 h结果无显著性差异(P>0.05);测定食品中大肠菌群时ONPG培养基18 h结果与LST肉汤48 h结果无显著性差异(P>0.05)。结论 ONPG培养基可用于快速测定食品中大肠菌群。

  20. Supplerende basisundersøkelser og rutineovervåking i Iddefjorden 1983. Testing av Iddefjordens termotolerante coliforme bakterieflora for innhold av termotolerante Klebsiella

    Ormerod, K.

    1984-01-01

    Det er gjentatte somrer obsrvert at antallet av termotolerante coliforme bakterier, TCB, i Iddefjorden synker i fellesferien når utslippene fra treforedlingsindustrien er midlertidig opphørt. Denne undersøkelsen viser at hovedmengden av TCB i Iddefjorden før fellesferien tilhørte slekten Klebsiella og Enterobacter, og at Klebsiella forsvant fra fjorden i løpet av fellesferien. Enkelte arter av Klebsiella forårsaker urin- og luftveisinfeksjoner hos mennesker, og utgjør derfor en annen hygienis...

  1. Effect of oral administration of Bacillus coagulans B37 and Bacillus pumilus B9 strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model

    Lopamudra Haldar; Gandhi, D.N.

    2016-01-01

    Aim: To investigate the effect of oral administration of two Bacillus strains on fecal coliforms, Lactobacillus and Bacillus spp. in rat animal model. Materials and Methods: An in vivo experiment was conducted for 49-day period on 36 adult male albino Wister rats divided equally into to four groups. After 7-day adaptation period, one group (T1) was fed on sterile skim milk along with basal diet for the next 28 days. Second (T2) and (T3) groups received spore biomass of Bacillus coagulans B...

  2. Relación entre las cantidades de cobre y zinc administradas a pacientes graves con nutrición parenteral total y los niveles de cobre y zinc en plasma y eritrocitos Relationship between the amount of copper and zinc given to critically ill patients on total parenteral nutrition and plasma and erythrocyte copper and zinc levels

    A. M. Menéndez

    2008-08-01

    superiores a 1,2 mg/d.Objectives: Complications resulting from zinc and copper deficiency, or adverse effects from excessive zinc and copper intake should be avoided during total parenteral nutrition (TPN. This study was conducted on critically ill patients requiring TPN to determine the relationship between the zinc and copper levels of the TPN mixtures, patients' clinical progression, and changes in plasma levels of zinc, serum levels of copper, and erythrocyte levels of zinc and copper. Patients and methods: 29 adult critically ill patients following pancreatitis or after a major abdominal surgery were studied. Zinc and copper levels in TPN, plasmatic zinc levels, copper serum levels and erythrocyte levels of zinc and copper were determined at the onset and at the end of the treatment (5-21 days (using Atomic Absorption Spectrometry. Results: The mean ± standard deviation (and ranges in parenthesis of zinc and copper levels in TPN were (μg/mL: zinc: 4.2 ± 1.7 (1.8 a 9.3; copper: 0.94 ± 0.66 (0.1 a 3.1. Biochemical parameters at the onset and at the end of the treatment were, respectively: (μg/mL: plasmatic zinc: 80 ± 45 (29-205 and 122 ± 56 (37-229; erythrocyte zinc: 2,300 ± 1,070 (790-5,280 and 2,160 ± 920 (790-4,440; serum copper (μg/dL: 124 ± 35 (62-211 and 128 ± 41 (60- 238; erythrocyte copper (μg/dl: 72 ± 39 (4-183 and 70 ± 41 (9-156. Plasmatic and erythrocyte zinc levels did not correlated neither at the onset nor at the end of the treatment. Changes in erythrocyte zinc levels correlated with daily administered zinc (mg/d in the parenteral nutrition (r = 0.38. Serum copper and erythrocyte copper showed significant correlation at the onset (p = 0.0005 and at the end of treatment (p = 0.008. Changes of serum or erythrocyte copper levels showed a significant correlation with daily administered copper (r = 0.31 and 0.26, respectively. Conclusions: These results show that: 1 determination of erythrocyte zinc and copper levels in these critically ill

  3. The discuss on quality control methods of culture medium for thermotolerant coliform bacteria%检测耐热大肠菌群培养基的质量控制方法探讨

    钟宁

    2012-01-01

      The quality of culture medium is the foundation of the microbial detection, affecting directly the reliability of the test results. In this paper, combining with the actual situation, the discuss on quality control of the EC culture medium for thermotolerant coliform bacteria was carried out through the outside,pH value, asepsis check, sensitivity check and others aspects so as to microbiology laboratory perfect and improve continuously culture medium quality level , and to ensure the accuracy of the test results.%  培养基的质量是微生物检测工作的基础,直接影响检测结果的可靠性。本文结合实际情况,从外观、pH值、无菌检查及灵敏度检查等几个方面,对检测耐热大肠菌群所用EC培养基的质量控制进行讨论,以便微生物实验室不断完善和提高培养基的质控水平,从而保证检验结果的准确性。

  4. Study on the GB 4789.3-2010 MPN Method Fitting in Coliform Bacteria Current Indicators of Pickled Vegetables%GB 4789.3-2010 MPN 法适合酱腌菜大肠菌群现行指标的研究

    刘耕典; 李宏梁; 舒静; 王涛; 杨雯

    2016-01-01

    实验采用 GB/T 4789.3-2003方法与 GB 4789.3-2010的 MPN(最可能数)计数法对散装酱腌菜与袋装酱腌菜共90个样品中的大肠菌群进行检测,并结合2010版国家标准对2003版大肠菌群的限量指标进行替换来对比两类酱腌菜的检测结果,在此基础上,对两类酱腌菜的不合格率进行了分析。结果表明:两类酱腌菜中,2003版大肠菌群限量指标分别为90,30 MPN/100 mL(g),替换后的2010版大肠菌群限量指标分别为0.9,0.3 MPN/g(mL),两种方法检测袋装酱腌菜均为大肠菌群阴性,即合格样品。散装酱腌菜2003版的不合格率为8.6%,替换后的2010版不合格率为11.4%,二者差值小于5%,其原因在于2003版与2010版检测方法的灵敏度有差异,因此替换后的检测结果对酱腌菜的不合格率未产生影响。研究结果可为食品中大肠菌群限量指标的确定提供一定依据。%In the present study,the coliform bacteria from bulk pickles and bag pickles (totaling 90 samples)are detected by two methods,namely GB/T 4789.3 -2003 method and GB 4789.3 -2010 MPN enumeration.The two methods are compared through replacing the coliform bacteria limited index of 2003 version with 2010 edition national standard.And then,the fraction defective of both kinds of pickles are analyzed.The results show that,as to the pickled vegetables,the coliform bacteria limited indicators of 2003 version are 90 MPN/100 mL (g )and 30 MPN/100 mL (g ) respectively.After being replaced as 2010 version,they are 0.9 MPN/g (mL)and 0.3 MPN/g (mL) respectively.The coliform bacteria detection result of bag pickles reveals negative by both detection methods,indicating they are qualified samples.The fraction defective of bulk pickles which analyzed by 2003 and 2010 versions are 8.6% and 11.4% respectively,indicating a difference value of less than 5%.The reason should be the sensitivity between both methods has

  5. Níveis de proteína na dieta de bovinos Nelore de três condições sexuais: consumo, digestibilidades total e parcial, produção microbiana e parâmetros ruminais Increasing crude protein levels in diets of Nellore cattle: intake, ruminal and total tract digestibility, microbial protein synthesis, and ruminal metabolism

    Robson Magno Liberal Véras

    2007-08-01

    .Twelve Nellore cattle fitted with ruminal and duodenal cannulas were used to investigate the effects of increasing dietary crude protein level (7, 10, 13, and 15% on intake, ruminal and total tract digestibility of nutrients, microbial protein synthesis, degradabilities of corn silage, corn starch, and cottonseed meal, and ruminal metabolism. Animals were blocked by sexual category (heifers, bulls and steers averaging 254.8, 285.1, and 265.6 kg of body weight, respectively and then assigned to one out of three Latin squares. Samples were incubated in the rumen of one animal of each sexual category to estimate ingredient degradability after determination of DM, CP and NDF in the incubation residues. Ruminal fluid was collected before and after-feeding to determine pH and concentration of ammonia while ruminal samples for bacterial isolation were taken 6 hours post-feeding. Blood (BUN and urine (UU were also collected for measurement of urea concentration. The degradation rates of DM and CP were both lower in corn silage than on corn starch and cottonseed meal. The interaction sexual condition x dietary crude protein level was not significant for nutrient intake. With the exception of EE and NFC, no other significant effect of dietary CP level was observed for daily intake of nutrients. Increasing dietary CP level affected total tract digestibilities of DM, OM, CP, NDF, and NFC and also resulted in higher concentrations of BUN and UU. A significant dietary crude protein level x collection time interaction was observed for ruminal NH3 but not for ruminal pH. It was concluded that increasing dietary CP levels affected intake and total tract digestibility of most nutrients.

  6. 全局过程公用工程系统改造顶层分析法及其应用%Improved philosophy of top level analysis for total site energy integration retrofit

    范继宽; 尹洪超

    2001-01-01

    对全局过程节能改造的能量集成方法进行了分析研究;提出了改进的顶层分析法,对公用工程系统剩余热的热功转化途径进行了分析和改进;采用了全局过程产用蒸汽等级优化,使过程改造的全局能量集成始终以经济效益为判据,由外及里层层深入,以较少的过程数据和工作量完成复杂的能量集成改造分析.应用实例表明,改进的顶层分析法用于复杂过程系统节能改造方案的确定是非常有效的.%An improved philosophy of top level analysis is proposed fortotal site energy integration retrofit. It is shown that steam saving or more generating from processes creates a surplus of steam in the total site. Paths for a more efficient use of this surplus steam to generate more power are identified with the help of a path-analysis, in which the difference of electricity power generating turbines and process turbines is taken into account. The improved approach not only provides powerful insights into the utility system economics, but also is a useful tool for evaluation of steam saving, steam generating, and steam switching projects under the condition of fixed configuration of utility network. Furthermore, it rules out uneconomic options, and reduces the time and effort for data extraction for project identification. A practical example of total site utility retrofit shows that the improved path-analysis is effective.

  7. Free and total testosterone levels in field males of Octodon degus (Rodentia, Octodontidae: accuracy of the hormonal regulation of behavior Niveles de testosterona libre y totales en machos silvestres de Octodon degus (Rodentia, Octodontidae: exactitud de la regulación hormonal del comportamiento

    MAURICIO SOTO- GAMBOA

    2005-06-01

    Full Text Available Animals exhibit continuous seasonal changes in physiological, morphological and behavioral traits associated to their natural annual cycles. One of the most important changes in the organism occurs at the initiation of breeding season. In males these changes include activation of spermatogenesis, enhance of sexual secondary characters and an increase of aggressiveness. All of theses changes are basically regulated by testosterone, but the physiological basis of this testosterone regulation and the effective hormone proportion that acts in target tissues are unknown. In this work I evaluated the relationship between total testosterone, free testosterone associated to aggressive behavior in wild males of Octodon degus. I compared hormonal levels and aggressive behavior during pre, middle and post breeding periods. Results showed that behavioral aggressiveness was correlated with both total and free testosterone in June, during the beginning of breeding season, but not at other times. Results also indicated a lack of relationship between free and total testosterone in most of breeding period. I discuss the importance of this behavior-hormonal regulation (determined by free testosterone and the physiological importance of this mechanism to the organismLos animales presentan variaciones estacionales continuas que incluyen cambios fisiológicos, morfológicos y conductuales, asociados al ciclo anual. Estos cambios generalmente están activados por factores endógenos o exógenos, pero son regulados por el sistema endocrino de organismo. Uno de los cambios más importantes ocurre en el inicio del periodo reproductivo. Durante este periodo los machos presentan cambios asociados a la producción de esperatozoides, exacerbación de caracteres sexuales secundarios y un aumento de la agresividad. Todos estos cambios son regulados en parte por los niveles de testosterona, sin embargo, los mecanismos fisiológicos de esta regulación son desconocidos. En este

  8. Níveis de aminoácidos sulfurados totais para poedeiras semi-pesadas após a muda forçada Total sulfur amino acids levels for semi heavy weight laying hens after forced molt

    Antonio Carlos de Laurentiz

    2005-02-01

    Full Text Available Cento e sessenta e duas poedeiras semipesadas anteriormente submetidas à muda forçada foram distribuídas em um delineamento inteiramente ao acaso, com três tratamentos e nove repetições de seis aves para cada. Os tratamentos consistiram no fornecimento de rações contendo 0,45, 0,60 e 0,75% de aminoácidos sulfurados totais (AAST durante 30 dias após o término da muda forçada (fase de pós-muda. Após os 30 dias, cada um dos tratamentos adotados na fase de pós-muda foi desmembrado nos mesmos níveis de AAST, compondo, portanto, um delineamento inteiramente ao acaso em esquema fatorial 3x3 (3 níveis de AAST da fase pós-muda - 0,45; 0,60 e 0,75% x 3 níveis de AAST na fase de produção - 0,45; 0,60 e 0,75%, sendo os tratamentos nesta fase compostos de três repetições de seis aves cada. Na fase de pós-muda, o menor nível de AAST determinou menores valores para consumo de ração (POne hundred and sixty two brown laying hens after the induced molt were randomly assigned to three treatments with nine replications and six birds each. The treatments consisted of 0.45, 0.60 and 0.75% of total sulfur amino acids (TSAA in the diets for 30 days after the end of induced molt (postmolt phase. After 30 days, each one of the treatments used in the postmolt phase was dismembered in the same TSAA levels, composing therefore a completely randomized desing in a factorial arrangement 3x3 (3 levels of TSAA in the postmolt phase - 0.45, 0.60 and 0.75% x 3 TSAA levels in the production phase - 0.45, 0.60 and 0.75%. The treatments in the production phase were composed of three replications of six birds each. At the production phase the performance and egg quality were evaluated for 4 periods of 21 days. At postmolt phase the lower TSAA level (0.45% determined lower values (P<0.01 for feed intake, TSAA intake and body weight. The TSAA levels used in the postmolt phase affected (P<0.05 only the egg weight and the smaller weight were determined by 0

  9. 大肠菌群MPN计数法与CFU计数法相关性初探%Investigation of Correlation Between Coliform Bacteria MPN and CFU Counting Methods

    孙慧; 李天添

    2012-01-01

    Coliform bacteria are common and important food quality evaluation index. Today, there are two parallel units of MPN and CFU in both detection of coliform bacteria and limitation of national standards of various products. In this paper, combined the principles of these two detection techniques with author's own experience in food inspection for many years, the correlation between MPN and CFU were investigated.%大肠菌群是常见且重要的食品质量评价指标。如今,大肠菌群检验方法和各类产品国家限量标准值都存在MPN、CFU两种计数单位并行的现象,结合两种技术方法的原理与作者多年从事食品检验的经验,对MPN单位与CFU单位的相关性进行初步探讨。

  10. Estimates of Nitrogen, Phosphorus, Biochemical Oxygen Demand, and Fecal Coliforms Entering the Environment Due to Inadequate Sanitation Treatment Technologies in 108 Low and Middle Income Countries.

    Fuhrmeister, Erica R; Schwab, Kellogg J; Julian, Timothy R

    2015-10-01

    Understanding the excretion and treatment of human waste (feces and urine) in low and middle income countries (LMICs) is necessary to design appropriate waste management strategies. However, excretion and treatment are often difficult to quantify due to decentralization of excreta management. We address this gap by developing a mechanistic, stochastic model to characterize phosphorus, nitrogen, biochemical oxygen demand (BOD), and fecal coliform pollution from human excreta for 108 LMICs. The model estimates excretion and treatment given three scenarios: (1) use of existing sanitation systems, (2) use of World Health Organization-defined "improved sanitation", and (3) use of best available technologies. Our model estimates that more than 10(9) kg/yr each of phosphorus, nitrogen and BOD are produced. Of this, 22(19-27)%, 11(7-15)%, 17(10-23)%, and 35 (23-47)% (mean and 95% range) BOD, nitrogen, phosphorus, and fecal coliforms, respectively, are removed by existing sanitation systems. Our model estimates that upgrading to "improved sanitation" increases mean removal slightly to between 17 and 53%. Under the best available technology scenario, only approximately 60-80% of pollutants are treated. To reduce impact of nutrient and microbial pollution on human and environmental health, improvements in both access to adequate sanitation and sanitation treatment efficiency are needed. PMID:26320879

  11. 小白菜表面粪大肠菌群的污染及其耐药性研究%Occurrence of Fecal Coliforms on Chinese Cabbages and Their Resistance to Antibiotics

    李琼; 李雅颖; 姚槐应; 葛超荣

    2014-01-01

    为明确粪大肠菌群对蔬菜的污染及其耐药性,对厦门市集美区主要菜市场及超市的小白菜进行抽样调查。采用平板计数和MPN法对小白菜表面的粪大肠菌群进行计数,并通过对分离的细菌进行药敏试验,揭示粪大肠菌群对常用抗生素的耐药性。结果显示平板计数粪大肠菌群数量平均为631.3 CFU·g-1,合格率达90.9%;MPN计数平均为48.2 MPN·g-1,合格率达87.9%。清洗处理后粪大肠菌群数量从564.7 CFU·g-1降到92.5 CFU·g-1;流水浸泡10 min后,粪大肠菌群数量从564.7 CFU·g-1降到99.2 CFU·g-1。药敏试验结果表明,粪大肠菌对青霉素和四环素的耐药率达到100%,对红霉素的耐药率达到85.0%,但对庆大霉素、阿米卡星和环丙沙星没有耐药性。研究表明清洗处理显著降低粪大肠菌群的数量,粪大肠菌群对常用抗生素具有一定程度的耐药性,其耐药谱较广。%Microbial contamination of vegetables has aroused public concerns. An investigation was carried out to examine occurrence and resistance to antibiotics of fecal coliforms on fresh Chinese cabbages collected from farmers-markets and supermarkets in Jimei district of Xiamen city, China. Fecal coliforms were quantified using both MPN(most-probable-number)and PC(plate count)methods. Two treat-ments(washing or immersion in water)were used to evaluate the effect of cleaning on the abundance of fecal coliforms. The resistance of the isolated fecal coliforms to six common antibiotics(gentamicin, amikacin, penicillin, erythromycin, tetracycline, and ciprofloxacin)was eval-uated using antimicrobial susceptibility testing. The results showed that average population of fecal coliforms from the samples was 631.3 CFU·g-1 by PC method, with qualified rate of 90.9%, whereas average population of fecal coliforms was 48.2 MPN·g-1 by MPN method, with qualified rate of 87.9%. Washing and 10 min immersion reduced the number of fecal

  12. Total parenteral nutrition

    ... medlineplus.gov/ency/patientinstructions/000177.htm Total parenteral nutrition To use the sharing features on this page, please enable JavaScript. Total parenteral nutrition (TPN) will help you or your child get ...

  13. Total Knee Replacement

    ... following total knee replacement include unlimited walking, swimming, golf, driving, light hiking, biking, ballroom dancing, and other ... to the final success of your surgery. To learn more about the full value of total knee ...

  14. Total iron binding capacity

    ... page: //medlineplus.gov/ency/article/003489.htm Total iron binding capacity To use the sharing features on this page, please enable JavaScript. Total iron binding capacity (TIBC) is a blood test to ...

  15. Anatomic Total Shoulder System

    Full Text Available GLOBAL AP ANATOMIC TOTAL SHOULDER SYSTEM METHODIST HOSPITAL PHILADELPHIA, PA April 17, 2008 00:00:10 ANNOUNCER: ... you'll be able to watch a live global AP anatomic total shoulder surgery from Methodist Hospital ...

  16. TOTAL CHOLESTEROL, HIGH DENSITY LIPOPROTEINS (HDL AND CORTISOL PLASMA LEVELS, AND THEIR BIORHYTMICITY, IN 24 HOURS, THROUGHOUT YEAR, IN IDEAL-POLWARTH RAMS NÍVEIS PLASMÁTICOS DE COLESTEROL TOTAL, LIPOPROTEÍNAS DE ALTA DENSIDADE (HDL E CORTISOL, E SUA BIORRITMICIDADE, EM CARNEIROS IDEAL-POLWARTH

    Alcides de Amorim Ramos

    2006-12-01

    Full Text Available To evaluate the mean plasma concentrations of total cholesterol (TC, high density lipoproteins (HDL and cortisol, blood samples were collected of five Ideal-Polwarth rams, maintained at 22?53’S latitude, in semi-confinement, every two months throughout the year, by 24h period, with 2-hour intervals between colects. The TC, changed 40.70?1,11mg/dL (April and 61.48?1,11mg/dL (December, between months, while HDL changed 22.16?0.23mg/dL (December as 33.40?0.23mg/dL (February, but not make evident a circannual rhythm in this levels. The TC presented the lowest value at 16:30h (50.40?1.57mg/dL and the highest value at 8:30h collect (54.67?1.57mg/dL; the HDL lowest level was at 10:30h (27.04?0.33mg/dL and the highest level also at 8:30h collect (28.49?0.33mg/dL, however without permit circadian rhythm determination in your plasma concentrations. Similarly, the cortisol plasma concentrations, between collect months, presents variable, however without demonstrate circadian rhythm in this hormone secretion. In relation to different collection’s moments, throughout months, it wasn’t possible to define, by statistical analysis, a circadian rhythm of cortisol secretion. KEY WORDS: Ovine, adrenal hormone, biochemistry metabolites, circadian rhythm. Visando avaliar as concentrações médias de colesterol total (CT, lipoproteínas de alta densidade (HDL e cortisol plasmáticos, foram colhidas amostras de sangue de cinco carneiros Ideal-Polwarth, alocados em latitude 22°53’S, em regime de semiconfinamento, a cada dois meses, ao longo de um ano, com as colheitas em um período de 24 horas, e intervalos de duas horas entre elas. O CT oscilou entre 40,70±1,11mg/dL (abril e 61,48±1,11mg/dL (dezembro, entre os meses, enquanto HDL variou de 22,16±0,23mg/dL (dezembro a 33,40±0,23mg/dL (fevereiro, mas não evidenciando um ritmo circanual em seus níveis. O CT apresentou seu valor mínimo na colheita das 16h30min (50,40±1,57mg/dL e o máximo às 8h30min

  17. Total well dominated trees

    Finbow, Arthur; Frendrup, Allan; Vestergaard, Preben D.

    cardinality then G is a total well dominated graph. In this paper we study composition and decomposition of total well dominated trees. By a reversible process we prove that any total well dominated tree can both be reduced to and constructed from a family of three small trees....

  18. The enumeration of coliforms and E. Coli on naturally contaminated beef: A comparison of the petrifilm(™) method with the Australian Standard.

    Bloch, N; Sidjabat-Tambunan, H; Tratt, T; Bensink, J; Lea, K; Frost, A J

    1996-06-01

    Petrifilm(™) PEC was compared to Australian Standard (AS) methods for the enumeration of coliforms and Escherichia coli on 50 naturally contaminated beef samples from three meat works. The standard methods consist of a 3-tube most probable number test or a direct plate count on tryptone bile agar for E. coli, and violet red bile agar and the most probable number test for conliorms (AS 1766.2.12.1984 and AS 1766.2.3.1992). No significant difference was found between the methods, except that the count of E. coli on the direct plate method with a resuscitation step was significantly higher than the count on Petrifilm(™), most probable number or direct plate count without resuscitation. PMID:22060573

  19. Controversy Total War

    Segesser, Daniel Marc

    2014-01-01

    Total war is a controversial term used in the past by politicians, publicists and military officers as well as by computer specialists and academics in the present. Since its conception by French politicians during the First World War in a time of severe crisis (1916/17), it has become a term used by historians and other academics to cover a wide array of elements when looking at wars of the past. A real total war was and is impossible. Elements of total war – total war aims, total methods of...

  20. The Necessity of Enzyme Substrate Technique to Fecal Coliform in Environmental Monitoring%粪大肠菌群酶底物法在环境监测中的应用研究

    汤琳

    2014-01-01

    Fecal coliforms,used as indicator bacteria of water fecal pollution,play an important role in the assessment of surface wa-ter quality monitoring.Taking an accurate,fast detection method of fecal coliform has an important scientific significance for the oc-currence and spread of epidemic disease control.From the meaning of fecal coliform monitoring,monitoring method,standard appli-cation and other aspects,this article put forward the necessity of popularizing and standardizing the enzyme substrate technique to monitor fecal coliforms.%粪大肠菌群作为水体粪便污染的指示菌,对地表水的水质监测评价具有重要作用。采取较为精确、快速的粪大肠菌群检测方法,对控制流行疾病的发生和传播有重要的科学意义。从粪大肠菌群的检测意义、监测方法、标准化应用情况等方面进行归纳分析,提出推广和标准化酶底物法监测粪大肠菌群的迫切性。

  1. Research on Amount Various of Fecal Coliform in Bio-solids Autothermal Themophilic Aerobic Digestion%生物固体自热好氧消化中粪大肠杆菌数量的变化研究

    沈越; 张健

    2013-01-01

      自热好氧消化可杀灭生物固体中的粪大肠杆菌,使生物固体达到无害化和可资源化利用标准。文章研究了特定工艺条件下,粪大肠杆菌数量在生物固体自热好氧消化过程中的变化,结果表明,自热好氧消化过程中,当消化时间为26~50 h 时(平均温度为47℃),粪大肠杆菌数量呈大幅度下降的趋势,仅仅24 h,粪大肠杆菌数量减少了95.83%;消化进行到第68 h 时(温度为55℃),粪大肠杆菌的数量已达到“未检出”的水平,为生物固体快速资源化利用提供试验参考。%Fecal coliform in bio-solids can be inactivated in autothermal themophilic aerobic digestion, therefore, the bio-solids become harmlessness and reach the resource reuse standards. In fixed conditions, the paper researched the amount various of fecal coliform, the results showed that when the digestion time was 26~50 h (average temperature was 47 ℃) the amount of fecal coliform was heavily decreased, the amount of fecal coliform was decreased 95.83 % only in 24 h. When the digestion time was 68h (average temperature was 55 ℃), the amount of fecal coliform reached to not detected standard. The paper supplied research reference to bio-solids resource reuse quickly.

  2. Genoptraening efter total knaealloplastik

    Holm, Bente; Kehlet, Henrik

    2009-01-01

    The short- and long-term benefits of post-discharge physiotherapy regimens after total knee arthroplasty are debatable. A national survey including hospitals in Denmark that perform total knee arthroplasty showed a large variability in indication and regimen for post-knee arthroplasty rehabilitat......The short- and long-term benefits of post-discharge physiotherapy regimens after total knee arthroplasty are debatable. A national survey including hospitals in Denmark that perform total knee arthroplasty showed a large variability in indication and regimen for post-knee arthroplasty...

  3. Totalization Data Exchange (TDEX)

    Social Security Administration — The Totalization Data Exchange (TDEX) process is an exchange between SSA and its foreign country partners to identify deaths of beneficiaries residing abroad. The...

  4. Wave energy level and geographic setting correlate with Florida beach water quality.

    Feng, Zhixuan; Reniers, Ad; Haus, Brian K; Solo-Gabriele, Helena M; Kelly, Elizabeth A

    2016-03-15

    Many recreational beaches suffer from elevated levels of microorganisms, resulting in beach advisories and closures due to lack of compliance with Environmental Protection Agency guidelines. We conducted the first statewide beach water quality assessment by analyzing decadal records of fecal indicator bacteria (enterococci and fecal coliform) levels at 262 Florida beaches. The objectives were to depict synoptic patterns of beach water quality exceedance along the entire Florida shoreline and to evaluate their relationships with wave condition and geographic location. Percent exceedances based on enterococci and fecal coliform were negatively correlated with both long-term mean wave energy and beach slope. Also, Gulf of Mexico beaches exceeded the thresholds significantly more than Atlantic Ocean ones, perhaps partially due to the lower wave energy. A possible linkage between wave energy level and water quality is beach sand, a pervasive nonpoint source that tends to harbor more bacteria in the low-wave-energy environment. PMID:26892203

  5. Concentration levels of alpha emitting radionuclides in natural waters. Implications in the use of various radionuclides of reference for the determination of total alpha activity index; Niveles de concentracion de radionucleidos emisores alfa en aguas de origen natural. Implicaciones en el uso de los distintos radionucleidos de referencia para la determinacion del indice de actividad total alfa

    Corbacho, J. A.; Baeza, A.; Guillen, J.; Valles, I.; Serrano, I.; Camacho, A.; Montana, M.

    2013-07-01

    The present study was carried out a statistical evaluation of the natural radioactive contents of a large number of water samples collected at different points of the Spanish geography, in order to meet its controlling radioactive composition and assessing the implications of total alpha activity index can have in this type of samples. (Author)

  6. Remoção de matéria orgânica, de nutrientes e de coliformes no processo anaeróbio em dois estágios (reator compartimentado seguido de reator UASB para o tratamento de águas residuárias de suinocultura Organic matter, nutrients and coliforms removal in two-stage anaerobic process (anaerobic baffled reactor followed by UASB reactor for swine wastewater treatment

    Mário S. de Abreu Neto

    2009-03-01

    Full Text Available Neste trabalho, avaliou-se o efeito das águas residuárias de suinocultura, com concentrações médias de sólidos suspensos totais variando de 4.591 a 13.001 mg L-1, no desempenho de processo anaeróbio, em dois estágios, compostos por reator compartimentado (ABR e reator de fluxo ascendente com manta de lodo (UASB, instalados em série, em escala- -piloto (volumes de 530 e 120 L, respectivamente, submetidos a tempos de detenção hidráulica (TDH de 60; 36 e 24 h no primeiro reator, e de 13,6; 8,2 e 5,4 h no segundo reator. As eficiências médias de remoção de DQOtotal variaram de 69 a 84% no reator ABR e de 39 a 58% no reator UASB, resultando em valores médios de 87 a 94% para o sistema de tratamento anaeróbio em dois estágios, com carga orgânica volumétrica (COV na faixa de 11,5 a 18,0 g DQOtotal (L d-1 no reator ABR, e de 4,2 a 13,4 g DQOtotal (L d-1 no reator UASB. A produção volumétrica máxima de metano de 0,227 m³ CH4 (m³ reator d-1 ocorreu no reator UASB, com COV de 10,6 g DQOtotal (L d-1 e TDH de 5,4 h. As maiores eficiências de remoção de coliformes totais e termotolerantes (99,7%, DQOdiss (94%, SST (96%, NTK (71%, P-total (61% e outros nutrientes, no sistema de tratamento anaeróbio em dois estágios, foram obtidas com o TDH de 73,6 h e temperatura climatológica média de 24,6 °C, aplicando-se a menor COV (de 11,5 g DQOtotal (L d-1 no reator ABR, e de 4,2 g DQOtotal (L d-1 no reator UASB com a maior concentração de SST do afluente (13.001 mg L-1.In this work it was evaluated the effect of swine wastewater with mean total suspended solid (TSS concentration ranging from 4.591 to 13.001 mg L-1 on the performance of the anaerobic process in two stages composed of anaerobic baffled reactors (ABR and an upflow sludge blanket reactor (UASB, installed in series, in pilot scale testing (volumes of 530 and 120 L, respectively and with hydraulic detention times (HDT of 60; 36 and 24 h in the ABR reactor and 13.6; 8.2 and

  7. Acute effect of weight loss on levels of total bilirubin in obese, cardiovascular high-risk patients: an analysis from the lead-in period of the Sibutramine Cardiovascular Outcome trial

    Andersson, Charlotte; Weeke, Peter; Fosbøl, Emil Loldrup;

    2009-01-01

    Low levels of bilirubin are associated with an increased risk of cardiovascular adverse events. Weight reduction is known to reduce several cardiovascular risk factors, but effects on bilirubin levels have not been reported. We studied the response of weight loss therapy with sibutramine and life...

  8. Changes of serum levels of prealbumin (PAB), cholinesterase (CHE), total bile acid (TBA) and ALT as related to the severity of inflammatory process and hepatic fibrosis in patients with chronic virus B hepatitis

    Objective: To study the correlationship between the serum levels of PAB, CHE, TBA, ALT and the severity of the disease process in patients with chronic virus B hepatitis. Methods: Serum levels of PAB, CHE, ALT (with biochemical methods) and TBA (with RIA) were examined in 93 patients with biopsy proven virus B hepatitis and 46 controls. Results: The 93 patients were of two groups: a less advanced group (n=51) and a more advanced group (n=42). Serum TBA, ALT levels were significnatly higher and serum PAB, CHE levels were significantly lower in the more advanced group than those in the less advanced group (P0 to s4. Changes of levels of ALT were of no regular pattern, but serum levels of TBA regularly increased and levels of PAB, CHE regularly decreased as the fibrosis grading proceeded from s0 to s4 and the differences between the levels in s4 and any other grading were significant (P<0.01). Conclusion: Combined determination of these serum markers might reflect the degree of inflammatory process and hepatic fibrosis in patients with virus B hepatitis, leading to earlier detection of cirrhosis. (authors)

  9. Estado trófico e bioacumulação do fósforo total no cultivo de peixes em tanques-rede na área aqüícola do reservatório de Itaipu - DOI: 10.4025/actascibiolsci.v30i3.519 Trophic level and bioaccumulation of total phosphorus in cage fish rearing on the aquaculture area at Itaipu reservoir - DOI: 10.4025/actascibiolsci.v30i3.519

    Wilson Rogério Boscolo

    2008-10-01

    Full Text Available A intensa produção de peixes e uso dos recursos hídricos pode comprometer a qualidade e disponibilidade da água, além de afetar a capacidade de suporte do ambiente, causando eutrofização e conseqüentemente a poluição do mesmo. Realizou-se um estudo das variáveis físico-químicas da água e dos teores de fósforo total (P-total no sedimento, rações e peixes cultivados em tanques-rede em uma área aqüícola localizada no reservatório da Itaipu, no município de Santa Helena – PR, objetivando monitorar a qualidade da água e determinar o índice de eutrofização total da área de cultivo, além de analisar a bioacumulação de P-total nos peixes e sedimento. As variáveis físico-químicas da água mantiveram valores normais indicados pelo CONAMA. A concentração média de P-total na área aqüícola foi de 28,41 IET e com transparência por disco de Secchi de 2,74 m, enquadrando o ambiente como oligotrófico. Houve correlação de 0,55 (pThe intense production of fishes and the use of water resources can compromise the quality and the availability of water, besides its affects in the environment carrying capacity, causing eutrophization and consequently water pollution. Therefore the objective of this study was to monitor the physical-chemical water variables and to measure the total phosphorus (total-P in sediment, feed and fish in cage culture on an aquaculture area situated at the Itaipu reservoir in Santa Helena – Parana state, observing the water quality and determining the total eutrophic level on the aquaculture area, in addition to analyze the total-P bioaccumulation in fish and sediment. The physical-chemical water variables presented normal values as indicated from CONAMA. The mean concentration of total-P for the aquaculture area was 28.41 IET and Secchi disc transparency of 2.74 m, giving it an oligotrophic classification. There was a correlation of 0.55 (p<0.01 between this two variables. The bioaccumulation of

  10. Levels and treatment options for enteric and antibiotic resistant bacteria in sewage from Sisimiut, Greenland

    Jensen, Pernille Erland; Gunnarsdottir, Ragnhildur; Andersen, Henrik Rasmus;

    2013-01-01

    enterococci, the wastewater is very strong, suggesting a potential hygienic risk. In addition, a high fraction of antibiotic resistant bacteria and an increased toxicity in the sub-stream from the hospital, suggest that this stream contains toxic compounds, possibly antibiotic of nature that may affect the...... the presence of Total coliforms, Escherichia coli (Ecoli), enterococci, streptococci, antibiotic resistant enteric bacteria, and toxicity in sewage from two sewer outlets in Sisimiut, West-Greenland, as well as in a sub-stream from the local hospital. According to the content of streptococci and...... local Arctic marine environment negatively. Both peracetic acid treatment and UV-C radiation shows potential for disinfection of the wastewater after removal of solids >60μm. E-coli was most susceptible to peracetic acid treatment, while a maximum possible reduction of enterococci and coliforms of 2...

  11. Correlation of lipid profile levels with deoxyribonucleic acid damage and total antioxidant capacity levels in obese pregnant women%肥胖产妇血脂水平与 DNA 损伤及总抗氧化能力水平的相关性研究

    赵红霞; 董艳双; 蔡友治; 朱颖军

    2015-01-01

    Objective The article aimed to investigate the correlation of lipid profile levels with deoxyribonucleic acid (DNA) damage and total antioxidant capacity (TAC) levels in obese pregnant women. Methods Healthy pregnant women (n=120 ) who took routine prenatal care from August 2011 to August 2012 in our hospital were recruited .All the pregnant women were di-vided into normal weight group ( n =45 ) and obese group ( n=75 ) .The lipid profile levels , DNA damage and TAC levels of two groups were compared and analyzed , and the correlations among lipid profile levels , DNA damage and TAC levels were analyzed . Results Compared to normal weight group , obese group showed significantly higher levels of TC (P=0.000), TG(P=0.000), and LDL-c(P=0.004), but a lower level of HDL-c (P=0.006).The DNA damage and TAC level of obese group were obviously higher than those of normal weight group (P=0.000, P=0.000).The DNA damage was positively correlated with levels of TC , TG and LDL-c among obese pregnant women (r=0.23, P=0.026;r=0.26, P=0.008;r=0.19, P=0.032), and TAC level was positive-ly correlated with TG level (r=0.32,P=0.000). Conclusion Dyslipidemia, imbalance of prooxidant and antioxidant status al-ways occur to obese pregnant women .The DNA damage is positively correlated with levels of TC , TG and LDL-c among obese pregnant women, and TAC is positively correlated with TG level .%目的:血脂代谢异常所致的DNA损伤很可能与自然流产、胎膜早破、子痫前期等有着密切的关系。文中旨在探讨肥胖产妇血脂水平与DNA损伤及总抗氧化能力水平的相关性。方法选取2011年8月至2012年8月于天津医科大学第四中心临床学院门诊进行系统产科检查的妊娠女性120例为研究对象,按照体重指数( BMI )分为肥胖组( BMI≥28kg/m2,n=75)和正常组(BMI<28kg/m2且孕期体重增长≤15kg,n=45)。比较分析2组血脂水平、DNA损伤情况与总抗氧化能力水平,并分

  12. Rapid Detection of Coliforms Using Cell Culture Bottle%用细胞培养瓶法快速检测大肠菌群

    段学辉; 魏斌; 傅奇; 郭炳其; 贾奎艳

    2011-01-01

    研究了一种快速检测大肠菌群的细胞培养瓶剂法,即以国标法中的乳糖胆盐发酵培养基为基本营养成分,经浓缩、干燥后制成快速检测瓶剂,待检样品滴入细胞培养瓶内,培养24h,依据培养瓶盖上产气指示试纸颜色变化及瓶内溶液颜色变化,确定可疑阳性样品,并对其进行氧化酶实验,验证大肠菌群的存在.实验对细胞培养瓶法进行了大肠菌群的重复性及实际样品检测,并与国标法进行比较.结果显示:细胞培养瓶法与国标法所测结果符合率达96%,样品检测报告时间只需24h,比国标法减少48 h,2种方法所测结果在统计学上无显著性差异(P>0.05).细胞培养瓶法简单、快速、准确,利于基层监测机构和生产企业对食品中大肠菌群的快速检测.%A cell culture bottle method was developed for rapid detection of coliforms in food samples. In this study, lactose bile salt medium were concentrated and dried for bottle testing agent. According to the color change of the gas test strip and the solution in cell culture bottle, suspected positive samples could be determined, and then be verified the presence of coliforms in suspected samples through oxidize and microscopic examination. The results of repetitive tests and actual samples detection were compared with national standard method. A high agreement (96% ) was found between the cell culture bottle method and national standard method on 50 samples. The overall assay time of cell culture bottle method only needed 24 h, which reduced 48 h than that of national standard method. The statistical analysis showed that there werent significant difference (P >0. 05) between the examination results for these methods. As a simple,rapid and accurate detection method,cell culture bottle could meet the needs of the primary monitoring agency and manufacturers for rapid detection of coliforms in food.

  13. Anatomic Total Shoulder System

    Full Text Available ... with regard to longevity of total shoulders versus hips and knees. 00:46:20 GERALD WILLIAMS, MD: ... is almost ten years younger than the average hip or knee replacement patient. But if you look ...

  14. Anatomic Total Shoulder System

    Full Text Available GLOBAL AP ANATOMIC TOTAL SHOULDER SYSTEM METHODIST HOSPITAL PHILADELPHIA, PA April 17, 2008 00:00:10 ANNOUNCER: DePuy Orthopedics is continually advancing the standard of orthopedic patient care. In a few ...

  15. Anatomic Total Shoulder System

    Full Text Available ... MD: This is Doug Boardman from Richmond, Virginia. We're here at Methodist Hospital in Philadelphia, where ... will be performing a total shoulder arthroplasty. Before we go to Dr. Williams, I'd like to ...

  16. Anatomic Total Shoulder System

    Full Text Available ... you look anyplace, there's probably six or eight articles in the literature right now that have compared ... Dr. Rockwood, my friend and mentor, published an article that showed pain relief was better in totals. ...

  17. Anatomic Total Shoulder System

    Full Text Available ... We had a question with regard to physical therapy after total shoulder arthroplasty: expectations and limitations. 00: ... opposite. We don't -- we do hardly any therapy at all to reverse. With reverse patients, they ...

  18. Total 2004 results

    This document presents the 2004 results of Total Group: consolidated account, special items, number of shares, market environment, adjustment for amortization of Sanofi-Aventis merger-related intangibles, 4. quarter 2004 results (operating and net incomes, cash flow), upstream (results, production, reserves, recent highlights), downstream (results, refinery throughput, recent highlights), chemicals (results, recent highlights), Total's full year 2004 results (operating and net income, cash flow), 2005 sensitivities, Total SA parent company accounts and proposed dividend, adoption of IFRS accounting, summary and outlook, main operating information by segment for the 4. quarter and full year 2004: upstream (combined liquids and gas production by region, liquids production by region, gas production by region), downstream (refined product sales by region, chemicals), Total financial statements: consolidated statement of income, consolidated balance sheet (assets, liabilities and shareholder's equity), consolidated statements of cash flows, business segments information. (J.S.)

  19. Total Ecosystem Carbon Stock

    U.S. Geological Survey, Department of the Interior — Total ecosystem carbon includes above- and below-ground live plant components (such as leaf, branch, stem and root), dead biomass (such as standing dead wood, down...

  20. Anatomic Total Shoulder System

    Full Text Available ... And we have Dr. Song visiting us from South Korea. This patient is a 78-year-old male ... there's probably six or eight articles in the literature right now that have compared hemis to totals. ...

  1. Anatomic Total Shoulder System

    Full Text Available ... be performing a total shoulder arthroplasty. Before we go to Dr. Williams, I'd like to encourage ... It's always a question about how deep we go with this osteotomy. I tend to go almost ...

  2. Anatomic Total Shoulder System

    Full Text Available GLOBAL AP ANATOMIC TOTAL SHOULDER SYSTEM METHODIST HOSPITAL PHILADELPHIA, PA April 17, 2008 00:00:10 ANNOUNCER: DePuy Orthopedics is continually advancing the standard of orthopedic patient ...

  3. Total 2004 results

    NONE

    2005-02-01

    This document presents the 2004 results of Total Group: consolidated account, special items, number of shares, market environment, adjustment for amortization of Sanofi-Aventis merger-related intangibles, 4. quarter 2004 results (operating and net incomes, cash flow), upstream (results, production, reserves, recent highlights), downstream (results, refinery throughput, recent highlights), chemicals (results, recent highlights), Total's full year 2004 results (operating and net income, cash flow), 2005 sensitivities, Total SA parent company accounts and proposed dividend, adoption of IFRS accounting, summary and outlook, main operating information by segment for the 4. quarter and full year 2004: upstream (combined liquids and gas production by region, liquids production by region, gas production by region), downstream (refined product sales by region, chemicals), Total financial statements: consolidated statement of income, consolidated balance sheet (assets, liabilities and shareholder's equity), consolidated statements of cash flows, business segments information. (J.S.)

  4. Anatomic Total Shoulder System

    Full Text Available ... WILLIAMS, MD: The patients that have a total shoulder replacement will come in on their first postoperative visit and tell you their pain's gone. The hemi arthroplasty without interposition will come ...

  5. Anatomic Total Shoulder System

    Full Text Available ... compared hemis to totals. Even Dr. Rockwood, my friend and mentor, published an article that showed pain ... reasonably good cortices, I get a little more aggressive, but not a lot. This is not a ...

  6. Anatomic Total Shoulder System

    Full Text Available ... to longevity of total shoulders versus hips and knees. 00:46:20 GERALD WILLIAMS, MD: Well, obviously, ... ten years younger than the average hip or knee replacement patient. But if you look at the ...

  7. Total parenteral nutrition

    Total parenteral nutrition (TPN) will help you or your child get nutrition from a special formula through a vein in the ... you can also eat and drink while getting nutrition from TPN. Your nurse will teach you how ...

  8. Co-digestion of swine excreta associated with increasing levels of crude glycerin

    Marco Antonio Previdelli Orrico Junior

    2016-03-01

    Full Text Available ABSTRACT The objective of this research was to evaluate the performance of anaerobic co-digestion of swine excreta associated with increasing doses of crude glycerin and different hydraulic retention times (HRT. A completely randomized design was adopted in a 3 × 4 factorial arrangement composed of three HRT (10, 17, and 24 days and four crude glycerin doses (0, 5, 10, and 15 g/100 g of total solids [TS], with four replications per treatment. The assessed parameters were: biogas production potential, reductions of volatile solids (VS, chemical oxygen demand (COD, and most probable number of total and thermotolerant coliforms. The biogas production per added VS presented quadratic effect at 17 and 24 days of HRT, with ideal doses of 5.5 and 5.9 g of crude glycerin/100 g TS, respectively. There was no difference among glycerin doses at 10-day HRT for VS reductions; however, at HRT of 17 and 24 days, there were differences, with greater reduction of 61.1% for 5 g of crude glycerin/100 g TS at 24-day HRT. The COD reduction values showed an effect among retention times, in which the 24-day HRT provided the best results. Reductions in coliforms were greater than 99%, with no difference among treatments. Addition of 5 to 6 g of crude glycerin/100 g TS with a 24-day HRT is more effective in biogas production and reduction of VS, COD, and coliforms from co-digestion of swine excreta.

  9. Total Grid Estimation

    R. Kelley Pace

    1998-01-01

    This article provides a matrix representation of the adjustment grid estimator. From this representation, one can invoke the Gauss-Mrkov theorem to examine the efficiency of ordinary least squares (OLS) and the grid estimator that uses OLS estimates of the adjustments (the "plug-in" grid method). In addition, this matrix representation suggests a generalized least squares version of the grid method, labeled herin as the total grid estimator. Based on the empirical experiments, the total grid ...

  10. Effects of Anaerobic Fermentation Temperature on Extermination of Fecal Coliform in Biogas Effluent%沼气发酵温度对沼液粪大肠菌群去除效果的研究

    李明; 魏晓明; 耿如林; 薛晓燕; 张庆东; 周磊

    2015-01-01

    Elimination of fecal coliforms in biogas effluent under either middle or high anaerobic fermentation temperature was studied to investigate whether the biogas effluent can be used to flush animal houses .After 28 d of anaerobic fermen-tation under 35°C, 99.1% of fecal coliforms in the raw swine manure , of which TS was 8%, were eliminated.Howev-er , the residual fecal coliforms concentration in the biogas effluent was still very high and cannot be used to flush the ani -mal houses.Under the high temperature of 45°C or 55°C, almost all of the fecal coliforms in the biogas effluents , dis-charged from the anaerobic fermentation of swine manure , was eliminated .The results showed that the biogas effluent dis-charged from the anaerobic fermentation under middle temperature still have high concentration of fecal coliforms .A short term treatment of anaerobic fermentation under high temperature should be applied to further eliminate the fecal coliforms in the biogas effluents and made it qualified to be used to flush the animal houses .%为探讨沼液作为畜舍回冲水的可能性,研究了沼气发酵温度对粪大肠菌群的杀灭效果。在35℃条件下对TS为8%的猪粪废水进行沼气中温发酵28天后,废水中99.1%的粪大肠菌群被杀灭;但沼液中残留的粪大肠菌群数依然很高,不能作为畜禽舍的回冲水使用。在45℃和55℃的条件下对猪粪沼气中温发酵所排出的沼液进行3天的沼气高温发酵后,沼液中残留的粪大肠菌几乎全部被杀灭。上述结果表明,沼气中温发酵所排出的沼液依然残留有大量粪大肠菌群,可采用短期沼气高温发酵对沼液进行再处理以满足沼液安全使用的要求。

  11. Níveis de lípides plasmáticos em ratos submetidos à esplenectomia total, ligadura simultânea dos vasos esplênicos e à esplenectomia subtotal com preservação do pólo inferior Plasmatic lipids levels in rats after total splenectomy, simultaneous ligature of the splenic vessels and subtotal splenectomy with inferior pole preservation

    Isabel Cristina Andreatta Lemos Paulo

    2005-10-01

    Full Text Available OBJETIVO: Verificar o efeito da esplenectomia total, da ligadura dos vasos esplênicos principais e da esplenectomia subtotal com preservação do pólo inferior, nos lípides plasmáticos de ratos alimentados com dieta-controle ou dieta acrescida com 2,5% de colesterol. MÉTODO: Foram utilizados 111 ratos Wistar, machos, pesando entre 273g e 427g, com idade aproximada de 12 semanas, assim distribuídos: Grupo 1, controle (N = 20, não operado; Grupo 2 (N = 20 submetido à manipulação do baço; Grupo 3 (N = 31 submetido à esplenectomia total; Grupo 4 (N = 20, ligadura simultânea da artéria e veia esplênicas; Grupo 5 (N = 20, esplenectomia subtotal com preservação do pólo inferior. Foram dosados os lípides plasmáticos, e os ratos foram distribuídos em dois subgrupos, de acordo com a dieta (Subgrupo A- dieta-controle; Subgrupo B - dieta acrescida com 2,5% de colesterol. Todos os animais foram submetidos à nova colheita de sangue após 90 dias do início do experimento. RESULTADOS: Os animais submetidos à esplenectomia total, independentemente do tipo de dieta, apresentaram aumento significante (p 0,05. Os animais submetidos à ligadura simultânea da artéria e veia esplênicas e à esplenectomia subtotal com preservação do pólo inferior e alimentados com dieta-controle, não apresentaram alterações nos níveis de lípides plasmáticos, exceto pelo aumento da HDL (p BACKGROUND: To verify the effect on plasmatic lipid level of rats feed with control diet and diet added with 2.5% pure cholesterol after total splenectomy, ligature of the splenic vessels and partial splenectomy with preservation of the inferior pole. METHODS: One hundred and eleven male Wistar rats weighting between 273 and 427g aged 12 weeks, were randomly divided into 5 groups. Group 1 (N = 20 control group was not submitted to surgery; Group 2 (N = 20 submitted to manipulation of the spleen; Group 3 (N = 31 total splenectomy; Group 4 (N = 20 simultaneous ligature

  12. Improvement of Sealing Technology of Enzyme Substrate Method in Measuring the Total Coliforms/Fecal coliforms%总大肠菌群/粪大肠菌群检测方法中酶底物法封口技术的改进

    李宗全; 黄芳; 许江

    2014-01-01

    通过试验,验证了在科立得固定底物技术酶底物法检测大肠菌群/粪大肠菌群的方法中,可使用家用电熨斗替换专用程控定量封口机进行封口,且经济、方便.

  13. 332份农村饮用井水总大肠菌群和耐热大肠菌群污染状况分析%Pollution Analysis of Total Coliform and Thermotolerant Coliform Bacteria in Rural Drinking Water (332 Samples)

    潘新明

    2009-01-01

    [目的]了解武汉市江夏区农村井水微生物污染状况.[方法]对332件农村饮用井水的水质按GB/T5750.12-2006规定的方法进行总大肠菌群和耐热大肠茵群检测.[结果]总大肠菌群阳性检出率为95.8%;耐热大肠菌群的检出率为73.2%.[结论]农村井水微生物污染主要为耐热大肠菌群,且污染比较严重,存在暴发肠道传染病的可能性.农村饮水安全问题应引起高度重视.

  14. Total Energy Monitor

    Friedrich, S

    2008-08-11

    The total energy monitor (TE) is a thermal sensor that determines the total energy of each FEL pulse based on the temperature rise induced in a silicon wafer upon absorption of the FEL. The TE provides a destructive measurement of the FEL pulse energy in real-time on a pulse-by-pulse basis. As a thermal detector, the TE is expected to suffer least from ultra-fast non-linear effects and to be easy to calibrate. It will therefore primarily be used to cross-calibrate other detectors such as the Gas Detector or the Direct Imager during LCLS commissioning. This document describes the design of the TE and summarizes the considerations and calculations that have led to it. This document summarizes the physics behind the operation of the Total Energy Monitor at LCLS and derives associated engineering specifications.

  15. The profile of antibiotics resistance and integrons of extended-spectrum beta-lactamase producing thermotolerant coliforms isolated from the Yangtze River basin in Chongqing

    The spreading of extended-spectrum β-lactamases (ESBL)-producing thermotolerant coliforms (TC) in the water environment is a threat to human health but little is known about ESBL-producing TCs in the Yangtze River. We received 319 ESBL-producing stains obtained from the Chongqing basin and we investigated antibiotic susceptibility, bla gene types and the presence of integrons and gene cassettes. 16.8% of TC isolates were ESBL-producing bacteria and blaTEM+CTx-M was the predominant ESBL type. 65.2% of isolates contained class 1 integrons, but only 3 carried intI 2. Gene cassettes were amplified and sequenced. aadA, drfA, cmlA, sat1, aar3 and two ORF cassettes were found. In conclusion, Yangtze River is heavily polluted by ESBL-producing TC bacteria and the combined bla gene type could enhance antibiotic resistance. Class 1 integrons were widespread in ESBL-producing isolates and play an important role in multi-drug resistance. Characterization of gene cassettes could reveal the dissemination of antibiotic resistance genes. - Yangtze River is heavily polluted by ESBL-producing TC bacteria and Class 1 integrons play an important role in multi-drug resistance.

  16. The Effect of Sludge Application-to-Planting Interval on the Number of Coliforms Recovered from Vegetables Grown on Sludge-Amended Soils

    Ngole, Veronica M.

    Studies were carried out to determine whether there is any difference in the health risk involved in growing carrots and spinach on sludge-amended soils when a 90-day sludge application-to-planting interval and sludge application-to-harvest intervals are used for further pathogen reduction. The health risk was determined by enumerating the Most Probable Number (MPN) of Faecal Coliform (FC) recovered from both vegetables and identifying the different types of enteric bacteria recovered at harvest. The spinach and carrots were grown on four different soil types unto which a 3 year old sludge (type 1 sludge) and three month old sludge (type 2 sludge) had been separately applied at different rates. Two sludge application-to-planting and sludge application-to-harvest interval were used. A higher number of FC were recovered from carrots (1.5 log10 MPN/10 g-1.8 log10 MPN/10 g) than spinach (sludge applied at the same rate. More FC was recovered when a 0±3 day sludge application-to-planting interval was used as opposed to a 90 day sludge application-to-planting interval. Soil type, sludge age and sludge application rate affected the amount of FC recovered. The implications of these results on the specification of time interval in Regulations guiding the growth of vegetables on sludge-amended soil are discussed.

  17. The profile of antibiotics resistance and integrons of extended-spectrum beta-lactamase producing thermotolerant coliforms isolated from the Yangtze River basin in Chongqing

    Chen Hao [Department of Environmental Hygiene, School of Military Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, Chongqing 400038 (China); Shu Weiqun, E-mail: west2003@sohu.co [Department of Environmental Hygiene, School of Military Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, Chongqing 400038 (China); Chang Xiaosong; Chen Jian; Guo Yebin; Tan Yao [Department of Environmental Hygiene, School of Military Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, Chongqing 400038 (China)

    2010-07-15

    The spreading of extended-spectrum {beta}-lactamases (ESBL)-producing thermotolerant coliforms (TC) in the water environment is a threat to human health but little is known about ESBL-producing TCs in the Yangtze River. We received 319 ESBL-producing stains obtained from the Chongqing basin and we investigated antibiotic susceptibility, bla gene types and the presence of integrons and gene cassettes. 16.8% of TC isolates were ESBL-producing bacteria and bla{sub TEM+CTx-M} was the predominant ESBL type. 65.2% of isolates contained class 1 integrons, but only 3 carried intI 2. Gene cassettes were amplified and sequenced. aadA, drfA, cmlA, sat1, aar3 and two ORF cassettes were found. In conclusion, Yangtze River is heavily polluted by ESBL-producing TC bacteria and the combined bla gene type could enhance antibiotic resistance. Class 1 integrons were widespread in ESBL-producing isolates and play an important role in multi-drug resistance. Characterization of gene cassettes could reveal the dissemination of antibiotic resistance genes. - Yangtze River is heavily polluted by ESBL-producing TC bacteria and Class 1 integrons play an important role in multi-drug resistance.

  18. Estado actual de contaminación con coliformes fecales de los cuerpos de agua de la Península de Osa

    Jesús Mora

    2010-12-01

    Full Text Available La contaminación de los cuerpos de aguaen Costa Rica y en todo el mundo es unode los principales problemas ambientales,debido a la importancia estratégicade este recurso. El recurso hídrico esun elemento esencial no solo para lapreservación de la vida, sino también parala conservación de la flora y fauna de laregión.Su conservación y su calidad estánestrechamente vinculadas, prácticamente,a todas las actividades económicas ysociales en forma ineludible, así como a lasalud de su población.La Península de Osa se ha caracterizadopor ser una zona con una exuberantebiodiversidad, tanto en su flora comoen su fauna, raramente encontrada enuna zona pequeña.En 1975, se creó elParque Nacional de Corcovado,el más importante parque de Costa Rica y queprotege a un tercio de la península. Esteexótico territorio que es la Península deOsa no está exento de amenazas, debido ala fragmentación y destrucción del hábitat,la deforestación por la explotación de lamadera y el mal planeamiento en el uso dela tierra, la extracción ilegal de recursos,entre ellos el oro, y el crecimiento de lapoblación en sus alrededores.El Reglamento para la evaluación y laclasificación de la calidad de cuerpos deagua superficiales establece como límiteen el contenido de coliformes fecalesen el agua para actividades recreativasde contacto primario, un valor de 1000(NMP/100mL. Este mismo valor máximose aplica para el riego de hortalizas yotros alimentos que se consumen crudos;mientras que para el uso en abrevaderosy actividades pecuarias el valor máximopermisible de coliformes fecales es de2000, por mencionar algunos casos.Se realizó un estudio sobre la contaminaciónmicrobiológica, utilizando como indicador                                                          el contenido de coliformes fecales                                                   

  19. Defined Substrate Technology for Rapid Enumeration of coliforms in Water%特定底物技术快速检测水中大肠菌群相关指标

    刘路; 卢益新

    2004-01-01

    大肠菌群(Coliforms)、耐热大肠菌群(thermotolerant coliforms)和埃希氏大肠杆菌(E.Coli)都是判断水体是否存在粪便污染的重要微生物指标.文中介绍了一种特定底物技术可在24h之内同时检测大肠菌群和埃希氏大肠杆菌数量,如果提高培养温度至44.5±0.2℃,则可检测耐热大肠菌群数量.

  20. Quantificação de coliformes, Staphylococcus aureus e mesófilos presentes em diferentes etapas da produção de queijo frescal de leite de cabra em laticínios Enumeration of coliforms, Staphylococcus aureus and aerobic mesofilic bacteria throughout the manufacture process of a goat unripened cheese produced in a dairy plant

    Simone Ulrich Picoli

    2006-03-01

    Full Text Available O objetivo do presente estudo foi acompanhar a produção de queijo frescal de leite de cabra, avaliando a qualidade higiênica do processamento pela quantificação de coliformes, S. aureus e mesófilos totais. A produção de três diferentes lotes de queijo foi acompanhada, sendo coletadas amostras de várias etapas do processamento. Além disso, amostras de queijo pertencente ao lote acompanhado foram coletadas na prateleira de um estabelecimento comercial durante seu período de validade. Os suabes coletados foram semeados em ágar sangue ovino; as amostras de água foram submetidas à Colimetria; as demais amostras foram submetidas à contagem de S. aureus, coliformes e mesófilos totais por meio de protocolos de rotina. Verificou-se que, apesar da pasteurização ter diminuído consideravelmente as populações microbianas presentes no leite cru, a falta de sanificação adequada de um equipamento que entrava em contato com o leite cru e que dá acesso ao tanque de coagulação, resultou na recontaminação da matéria-prima. Ao final do processamento, o queijo encontrava-se dentro dos padrões exigidos pela legislação, contudo a elevada contagem de mesófilos totais sugere que sejam melhoradas as medidas de sanitização durante o processamento, a fim de garantir a qualidade higiênica e uma maior vida de prateleira ao queijo produzido.The aim of this study was to evaluate the hygienic conditions throughout the manufacture process of a goat unripened cheese, using the enumeration of coliforms, S. aureus and aerobic mesofilic bacteria as indicators. The production of three different lots of cheese was followed, being collected samples throughout the processing steps. Furthermore, samples of cheese belonging to the same sampled lot were collected in the shelf of a commercial establishment throughout the shelf life period. Swabs were streaked onto blood agar plates; water samples were submitted to colimetric assays; all other samples were

  1. Anatomic Total Shoulder System

    Full Text Available ... AP ANATOMIC TOTAL SHOULDER SYSTEM METHODIST HOSPITAL PHILADELPHIA, PA April 17, 2008 00:00:10 ANNOUNCER: DePuy ... you don't make a bunch of small passes at the lesser tuberosity and make it a ...

  2. Total Synthesis of (-)-Cardiopetaline.

    Nishiyama, Yoshitake; Yokoshima, Satoshi; Fukuyama, Tohru

    2016-05-20

    The total synthesis of (-)-cardiopetaline, an aconitine-type natural product, has been accomplished. Our synthesis involved a Wagner-Meerwein rearrangement of a sulfonyloxirane that enabled, in a single step, the construction of the bicyclo[3.2.1] system in the aconitine skeleton and effective introduction of oxygen functional groups at the appropriate positions. PMID:27166640

  3. Supravaginal eller total hysterektomi?

    Edvardsen, L; Madsen, E M

    1994-01-01

    There has been a decline in the rate of hysterectomies in Denmark in general over the last thirteen years, together with a rise in the number of supravaginal operations over the last two years. The literature concerning the relative merits of the supravaginal and the total abdominal operation is ...... indicate a reduced frequency of orgasm after the total hysterectomy compared with the supravaginal operation. When there are technical problems peroperatively with an increased urologic risk the supravaginal operation is recommended....... examined. It is concluded that the risk of developing carcinoma of the cervical stump is low, and no longer a weighty indication for the total in preference to the supravaginal hysterectomy as long as subsequent screening of the cervix is performed. At the same time it is important to inform the women......There has been a decline in the rate of hysterectomies in Denmark in general over the last thirteen years, together with a rise in the number of supravaginal operations over the last two years. The literature concerning the relative merits of the supravaginal and the total abdominal operation is...

  4. Total Cost of Ownership

    Zachariassen, Frederik

    2007-01-01

    Total Cost of Ownership (TCO), som giver et bud på, hvordan virksomheder kan opnå en bedre indsigt i, hvilke leverandører der forårsager hvilke omkostninger og dermed danne et forbedret beslutningsgrundlag for besparelser i leverandørleddet. I artiklen argumenteres først og fremmest for, hvorfor TCO er...

  5. Anatomic Total Shoulder System

    Full Text Available ... to longevity of total shoulders versus hips and knees. 00:46:20 GERALD WILLIAMS, MD: Well, obviously, if you look at -- it depends upon who you put them in. First of all, the young-- the youngest patient population for joint replacement in the United States by far, by ...

  6. Anatomic Total Shoulder System

    Full Text Available ... Anatomic Total Shoulder surgery, which featured the latest innovation in shoulder surgery from DePuy Orthopedics. OR-Live makes it easy for you to learn more. Just click on the "Request Information" button on your webcast screen and open the door to informed medical care. 01:21: ...

  7. "Total Deposition (TDEP) Maps"

    The presentation provides an update on the use of a hybrid methodology that relies on measured values from national monitoring networks and modeled values from CMAQ to produce of maps of total deposition for use in critical loads and other ecological assessments. Additionally, c...

  8. Anatomic Total Shoulder System

    Full Text Available ... put totals in unless they're under the age of 50. But as the patient gets older, ... I'll give you an example: 26-year-old juvenile rheumatoid arthritic. You can pulse-irrigate that, ...

  9. Anatomic Total Shoulder System

    Full Text Available ... advancing the standard of orthopedic patient care. In a few moments, you'll be able to watch a live global AP anatomic total shoulder surgery from Methodist Hospital in Philadelphia. A revolution in shoulder orthopedics, the Global AP gives ...

  10. Total versus subtotal hysterectomy

    Gimbel, Helga; Zobbe, Vibeke; Andersen, Anna Birthe; Gluud, Christian; Ottesen, Bent S; Tabor, Ann

    2005-01-01

    The aim of this study was to compare total and subtotal abdominal hysterectomy for benign indications, with regard to urinary incontinence, postoperative complications, quality of life (SF-36), constipation, prolapse, satisfaction with sexual life, and pelvic pain at 1-year postoperative. Eighty...

  11. Anatomic Total Shoulder System

    Full Text Available ... by almost ten years, is shoulders. So by definition, the average shoulder-replacement patient is almost ten ... Anatomic Total Shoulder surgery, which featured the latest innovation in shoulder surgery from DePuy Orthopedics. OR-Live ...

  12. Total Cross Sections

    G. GiacomelliBologna University and INFN

    2014-01-01

    The measurements of the hadron-hadron total cross sections are the first measurements performed when a new hadron accelerator opens up a new energy region; the measurements were made as function of the incoming beam momentum or c.m. energy and have often been repeated with improved accuracy and finer energy spacing.

  13. CSF total protein

    CSF total protein is a test to determine the amount of protein in your spinal fluid, also called cerebrospinal fluid (CSF). ... The normal protein range varies from lab to lab, but is typically about 15 to 60 mg/dL. Note: mg/dL = ...

  14. Total 2004 annual report

    This annual report of the Group Total brings information and economic data on the following topics, for the year 2004: the corporate governance, the corporate social responsibility, the shareholder notebook, the management report, the activities, the upstream (exploration and production) and downstream (refining and marketing) operating, chemicals and other matters. (A.L.B.)

  15. Total Quality Management Seminar.

    Massachusetts Career Development Inst., Springfield.

    This booklet is one of six texts from a workplace literacy curriculum designed to assist learners in facing the increased demands of the workplace. The booklet contains seven sections that cover the following topics: (1) meaning of total quality management (TQM); (2) the customer; (3) the organization's culture; (4) comparison of management…

  16. Total Quality Management Simplified.

    Arias, Pam

    1995-01-01

    Maintains that Total Quality Management (TQM) is one method that helps to monitor and improve the quality of child care. Lists four steps for a child-care center to design and implement its own TQM program. Suggests that quality assurance in child-care settings is an ongoing process, and that TQM programs help in providing consistent, high-quality…

  17. THE UNCERTAINTY ASSESSMENT OF HOSPITAL SEWAGE FECAL COLIFORM TEST RESULTS%医院污水粪大肠菌群检测结果不确定度的评定

    商晓春; 赵雪琴; 帅慧群; 张睿

    2012-01-01

    目的 研究医院污水粪大肠菌群检测结果不确定度,找出影响检测结果的主要不确定因素.方法 通过现场采集污水进行粪大肠菌群检测,对检验结果的不确定度来源作出评定.结果 本次医院污水粪大肠菌群检测不确定度为0.1408.重复性限为1.5978,复现性限为1.5248.结论 不确定度的分析能有效掌握检测结果的可信程度和准确性,所建立的不确定度评定方法,适合于类似粪大肠菌群检验的不确定度评定.%Objective To study the uncertainty of fecal coliform test results of hospital sewage and to identify the main uncertainty factors which affect the test results. Methods On - site sewage collection was used to do fecal coliform tests and to assess the sources of uncertainty in the test results. Results The uncertainty of this fecal coliform test of hospital sewage was 0. 1408, the repeatability limit was 1. 5978 and the reproducibility limit was 1. 5248. Conclusion The analysis of uncertainty can effectively acquire the credibility and accuracy of test results. This established uncertainty assessment method is suitable for the uncertainty assessment of similar fecal coliform tests.

  18. Detecção de emissão espontânea de luz em ensaios de colimetria aplicados ao monitoramento de efluentes sanitários Spontaneous light emission in coliforms test applied to wastewater monitoring

    Samuel Ricardo dos Santos

    2011-03-01

    Full Text Available No presente trabalho avaliou-se o potencial do emprego da técnica biofotônica ao monitoramento da qualidade microbiológica de efluentes sanitários, por meio da detecção de emissão ultrafraca de luz em testes envolvendo bactéria do grupo coliforme. Foram acompanhados os padrões de emissão de luz em câmara escura com o uso de efluente doméstico, antes e após tratamento, incubados em meio nutritivo à base de lactose e lauril triptose. O controle foi efetuado com o uso de cepa de Escherichia coli (ATCC 25.922, tendo seu crescimento sido monitorado por emissão de luz em câmara escura com fotomultiplicador acoplado. Os dados demonstraram que o monitoramento microbiológico pode ser efetuado por meio técnica biofotônica, podendo ser aplicado, com respostas rápidas, ao monitoramento microbiológico de efluentes, por meio de testes envolvendo coliformes.The spontaneous light emission of living systems emerge as a promising methodology that applied to microbiological in monitoring water can lead to short-term analysis. The present study evaluated the potential of biophoton measurements applied to wastewater monitoring by using ultraweak light emission in coliform tests. The procedure is based on photon-counting measurements inside a dark-chamber, of wastewater samples, before and after treatment, inoculated in nutrient presence/absence medium (lactose and lauryl triptose broth. Strain of Escherichia coli (ATCC 25,922 was used in control tests by monitoring the light emission inside a dark-chamber with an acoplade photomultiplier. The data showed that microbiological monitoring can be done by photon-counting in real-time applied to microbiological wastewater monitoring using coliform test.

  19. Mercúrio total em cabelos: uma contribuição para se avaliar o nível de exposição em Poconé, Mato Grosso, Brasil Total mercury in hair: a contribution to the evaluation of mercury exposure levels in Poconé, Mato Grosso, Brazil

    Flávia Nogueira

    1997-10-01

    Full Text Available Ocorrem na Bacia do Rio Bento Gomes (MT, às margens do Pantanal Mato-grossense, cerca de sessenta garimpos de ouro, onde o mercúrio é utilizado de forma intensiva nos processos de produção, através da formação de amálgamas que facilitam a aglutinação de partículas finas. A queima do amálgama nem sempre é feita em sistemas fechados de recuperação, havendo, portanto, perda de vapor para a atmosfera. Este estudo traz resultados de análises de mercúrio total nos cabelos de quatro grupos de voluntários que vivem em Poconé (MT, e faz uma caracterização geral do ambiente no que se refere à presença do metal na água e no sedimento do Rio Bento Gomes. Um equipamento de detecção por fluorescência foi usado para a determinação das concentrações, e os resultados indicam que os valores são mais baixos do que os de outras populações de áreas de garimpo no Brasil. Indicam também que a via ocupacional parece ser a mais eficiente rota potencial de contaminação da população por mercúrio.There are some 60 gold-mining sites in the Bento Gomes River basin (Mato Grosso, at the border of the Mato Grosso Pantanal (Swamp, where mercury is used to agglutinate fine gold particles through amalgamation. During burning of the amalgam to release the gold, mercury vapor is lost to the atmosphere, since closed systems for mercury recovery are not always used. This study shows the results of total mercury analysis in hair from four volunteer groups living in Poconé, Mato Grosso, and presents a general environmental description pertaining to the presence of mercury in water and sediments from the Bento Gomes River. Atomic fluorescence was used for measuring mercury concentrations. The results were lower than for other populations from gold-mining areas in Brazil.

  20. CYPRINIDS TOTAL BLOOD PROTEINS DETERMINATION

    TANŢI PATRICHE

    2013-07-01

    Full Text Available In aquaculture to get a high production is conditioned by awareness and keeping of an unaltered health condition of the biological material. To be aware of the health condition of the biological material in a fish farm allows us to establish the preventive measures required to prevent spreading of a disease and the treatment to be applied in case that a mass disease occurs. The level of the total protein in serum is, first of all, a synthetically indicator of the nutritional condition of the organism, presenting, at the same time, ample qualitative and quantitative variations depending on species, age, sex, stage of sexual maturity, water temperature and especially in correlation with the health condition of fish. Modification in value of the total protein point out some metabolic perturbations in fish body.

  1. Distribution of bacteria and coliform bacteria in the Meretrix meretrix culture environment on Rudong Beach of Jiangsu Province%江苏如东滩涂环境中细菌总数和大肠菌群分布特征及污染评价

    夏培艳; 沈新强; 袁骐; 蒋玫

    2011-01-01

    The samples were collected in March and May 2010 from Rudong coastal areas,Jiangsu Province. The coliform group and bacteria,as well as the physical and chemical factors in the clams' culture environment were investigated respectively. At the same time,the distribution features,pollution degree and the correlation were analysed. The results showed that all of the sites we studied met the Seawater quality standard and the Marine sediment standard. The range of bacteria in surface water was 10 ~ 4.8 × 104/mL,and total coliforms was 0 ~ 3200/L , while that in sediments were 0.1 × 103 ~3 × 104/g and 0 ~ 3.80/g respectively. With the correlation analysis of the environmental factors,conclusions were that the total bacteria had obvious relativity with CODMn sulfide and inorganic nitrogen,and their correlation coefficient were 0. 994,0. 992 and 0. 983 respectively. The correlation coefficient between coliform group and CODMn. sulfide,inorganic nitrogen, phosphate were 0. 995,0.985,0.974 and 0. 931.%按照相关方法要求,分别于2010年3月、5月对江苏如东滩涂文蛤产地环境中大肠菌群和细菌总数以及各环境因子进行调查检测,分析研究该环境中细菌总数和大肠菌群的分布特征、污染程度以及与各环境因子的相关性,并用单因子污染指数法评价其受大肠菌群污染状况.结果表明,表层海水细菌总数范围10~4.8×104/mL,平均值为6.63 ×103/mL,大肠菌群范围0~3 200/L;沉积物中细菌总数为0.1×103~3×104/g,平均值为5.64×103/g,大肠荫群范围0~3.80/g.对各环境因子进行相关性分析,得知:该环境中,细菌总数与CODMn、硫化物以及无机氮含量有显著的相关性,相关系数R分别达到0.994、0.992和0.983;大肠菌群与CODMn、硫化物、无机氮以及磷酸盐含量也有较好的相关性,相关系数R分别为0.995、0.985、0.974和0.931.表层海水中大肠菌群有2个站位超标,其余站位均符合质量要求,沉积物中

  2. Analysis on Status and Trend of Fecal Coliform Contamination of Surface Water Drinking Water Sources%地表水饮用水水源地粪大肠菌群污染现状及趋势分析

    邱国良; 陈泓霖

    2016-01-01

    In this paper, Hengyang city 3 centralized drinking water source in recent four years (2012-2015 years), the pollution status and pollution trend of fecal coliform in water was studied� For nearly four years, the water quality of the fecal coliform group in the concentration range, average, exceeded the number, exceeding the maximum multiple of the discussion and analysis, it is concluded that the water quality of the water quality of fecal coliform group pollution status and trend of pollution, to strengthen drinking water source water quality monitoring and supervision.%本文对衡阳市城区3个集中式生活饮用水水源地近四年(2012-2015年)来,水质中粪大肠菌群的污染状况及污染趋势进行了分析研究。对近四年来各水源地水质中粪大肠菌群的浓度范围、平均值、超标次数、最大超标倍数进行了讨论分析,得出了水源地水质中粪大肠菌群的污染现状及污染趋势,有利于加强饮用水水源地水质监测与监管。

  3. Total 2003 Results

    This document presents the 2003 results of Total Group: consolidated account, special items, number of shares, market environment, 4. quarter 2003 results, full year 2003 results, upstream (key figures, proved reserves), downstream key figures, chemicals key figures, parent company accounts and proposed dividends, 2004 sensitivities, summary and outlook, operating information by segment for the 4. quarter and full year 2003: upstream (combined liquids and gas production by region, liquids production by region, gas production by region), downstream (refinery throughput by region, refined product sales by region, chemicals), impact of allocating contribution of Cepsa to net operating income by business segment: equity in income (loss) and affiliates and other items, Total financial statements: consolidated statement of income, consolidated balance sheet (assets, liabilities and shareholder's equity), consolidated statements of cash flows, business segments information. (J.S.)

  4. Niveles de Partículas Suspendidas Totales (PST, PM10 y PM2.5 y su Relación en Lugares Públicos de la Ciudad Riohacha, Caribe Colombiano Levels of total suspended particles (TSP, PM10 and PM2.5 and their relationship in public places of the city Riohacha, Colombian Caribbean

    Roberto E Rojano

    2013-01-01

    Full Text Available Se determinaron las concentraciones de partículas suspendidas totales (PST y partículas menores de 10 y 2.5 micrómetros (PM10 y PM2.5 en la zona urbana del municipio de Riohacha, Colombia. Los instrumentos utilizados fueron un muestreador de alto volumen para PST y PM10 y un muestreador de bajo volumen (Partisol para las partículas PM2.5, todo en seis estaciones de monitoreo. Los resultados mostraron que el promedio de la concentración de PM10 varió desde 43,69 a 19,47 µg/m3, las PST de 86,02 a 27,38 µg/m3 y las PM2.5 mostraron un promedio de 14.57 µg/m3. Las relaciones PM10/PST variaron desde 0,50 a 0.68. Las seis estaciones presentaron buena correlación PST/PM10 (R = 0,795. Las PST pueden implicar presencia de partículas PM10 en el área urbana esta ciudad, pero las concentraciones de PM10 no necesariamente indican presencia de partículas PM2.5 (R= 0,035. Los resultados de correlación de PM10/PST son similares a estudios realizados en otras zonas urbanas de Colombia y de otros países.The concentrations of total suspended particles (TSP and particles with size below 10 and 2.5 micrometers (PM10 and PM2.5 in the urban area of the city of Riohacha in Colombia were determined. The instruments used were a high-volume sampler for TSP and PM10 and a low-volume sampler (Partisol for PM2.5, all in six monitoring stations. The results showed that the average PM10 concentrations ranged from 43.69 to 19.47 µg/m3, the PST ranged from 86.02 to 27.38 µg/m3 and the average for PM2.5 was 14.57 µg/m3. Relations PM10/PST ranged from 0.50 to 0.68. The six stations showed good correlation PST/PM10 (R=0.795. The PST can imply the presence of PM10 particles in the urban area of this city but PM10 concentrations not necessarily indicate the presence of PM2.5 particles (R=0.035. The results for the correlation PM10/PST are similar to studies conducted in other urban areas of Colombia and of other countries.

  5. Total - annual report 2005

    This annual report presents the activities and results of TOTAL S.A., french society on oil and gas. It deals with statistics, the managers, key information on financial data and risk factors, information on the Company, unresolved Staff Comments, employees, major Shareholders, consolidated statements, markets, security, financial risks, defaults dividend arrearages and delinquencies, controls and procedures, code of ethics and financial statements. (A.L.B.)

  6. Total Synthesis of Millingtonine.

    Brown, Patrick D; Lawrence, Andrew L

    2016-07-11

    Millingtonine is a glycosidic alkaloid that exists as a pair of pseudo-enantiomeric diastereomers. Consideration of the likely biosynthetic origins of this unusual natural product has resulted in the development of a seven-step total synthesis. Results from this synthetic work provide evidence in support of a proposed network of biosynthetic pathways that can account for the formation of several phenylethanoid natural products. PMID:27249628

  7. Total synthesis of teixobactin.

    Jin, Kang; Sam, Iek Hou; Po, Kathy Hiu Laam; Lin, Du'an; Ghazvini Zadeh, Ebrahim H; Chen, Sheng; Yuan, Yu; Li, Xuechen

    2016-01-01

    To cope with the global bacterial multidrug resistance, scientific communities have devoted significant efforts to develop novel antibiotics, particularly those with new modes of actions. Teixobactin, recently isolated from uncultured bacteria, is considered as a promising first-in-class drug candidate for clinical development. Herein, we report its total synthesis by a highly convergent Ser ligation approach and this strategy allows us to prepare several analogues of the natural product. PMID:27484680

  8. Total synthesis of ochnaflavone

    Monica M. Ndoile; van Heerden, Fanie R

    2013-01-01

    The first total syntheses of ochnaflavone, an asymmetric biflavone consisting of apigenin and luteolin moieties, and the permethyl ether of 2,3,2'',3''-tetrahydroochnaflavone have been achieved. The key steps in the synthesis of ochnaflavone were the formation of a diaryl ether and ring cyclization of an ether-linked dimeric chalcone to assemble the two flavone nuclei. Optimal experimental conditions for the oxidative cyclization to form ochnaflavone were established.

  9. Total synthesis of ochnaflavone

    Monica M. Ndoile

    2013-07-01

    Full Text Available The first total syntheses of ochnaflavone, an asymmetric biflavone consisting of apigenin and luteolin moieties, and the permethyl ether of 2,3,2'',3''-tetrahydroochnaflavone have been achieved. The key steps in the synthesis of ochnaflavone were the formation of a diaryl ether and ring cyclization of an ether-linked dimeric chalcone to assemble the two flavone nuclei. Optimal experimental conditions for the oxidative cyclization to form ochnaflavone were established.

  10. Boosting Total Relationship Marketing

    Aykut, Arslan

    2008-01-01

    This paper explores the track of marketing, by referring to the literature, through the advancements in digitalized technological systems particularly Information Technologies (IT), the emergence of a more broadly educated and more discerning consumer with increasing discretionary spending and purchasing power, the adoption of Total Quality Management (TQM) as the biggest competitive issue of 1990s, the growth of the service economy, the increase of competitive intensity and its impact on cus...

  11. Validation and use of a fast sample preparation method and liquid chromatography-tandem mass spectrometry in analysis of ultra-trace levels of 98 organophosphorus pesticide and carbamate residues in a total diet study involving diversified food types.

    Chung, Stephen W C; Chan, Benny T P

    2010-07-16

    This paper reports a comprehensive sensitive multi-residue liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection, identification and quantitation of 73 pesticides and their related products, a total of 98 analytes, belonging to organophosphorus pesticides (OPPs) and carbamates, in foods. The proposed method makes use of a modified QuEChERS (quick, easy, cheap, effective, rigged, and safe) procedure that combines isolation of the pesticides and sample clean-up in a single step. Analysis is performed by liquid chromatography-electrospray ionization-tandem mass spectrometry operated in the multiple reaction monitoring (MRM) mode, acquiring two specific precursor-product ion transitions per target compound. Two main fragment ions for each pesticide were obtained to achieve the identification according to the SANCO guidelines 10684/2009. The method was validated with various food samples, including edible oil, meat, egg, cheese, chocolate, coffee, rice, tree nuts, citric fruits, vegetables, etc. No significant matrix effect was observed for tested pesticides, therefore, matrix-matched calibration was not necessary. Calibration curves were linear and covered from 1 to 20 microg L(-1) for all compounds studied. The average recoveries, measured at 10 microg kg(-1), were in the range 70-120% for all of the compounds tested with relative standard deviations below 20%, while a value of 10 microg kg(-1) has been established as the method limit of quantitation (MLOQ) for all target analytes. Similar trueness and precision results were also obtained for spiking at 200 microg kg(-1). Expanded uncertainty values were in the range 21-27% while the HorRat ratios were below 1. The method has been successfully applied to the analysis of 700 food samples in the course of a baseline monitoring study of OPPs and carbamates. PMID:20557892

  12. Approaching zero: temporal effects of a restrictive antibiotic policy on hospital-acquired Clostridium difficile, extended-spectrum β-lactamase-producing coliforms and meticillin-resistant Staphylococcus aureus.

    Dancer, S J; Kirkpatrick, P; Corcoran, D S; Christison, F; Farmer, D; Robertson, C

    2013-02-01

    A restrictive antibiotic policy banning routine use of ceftriaxone and ciprofloxacin was implemented in a 450-bed district general hospital following an educational campaign. Monthly consumption of nine antibiotics was monitored in defined daily doses (DDDs) per 1000 patient-occupied bed-days (1000 pt-bds) 9 months before until 16 months after policy introduction. Hospital-acquired Clostridium difficile, meticillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase (ESBL)-producing coliform cases per month/1000 pt-bds were identified and reviewed throughout the hospital. Between the first and final 6 months of the study, average monthly consumption of ceftriaxone reduced by 95% (from 46.213 to 2.129 DDDs/1000 pt-bds) and that for ciprofloxacin by 72.5% (109.804 to 30.205 DDDs/1000 pt-bds). Over the same periods, hospital-acquisition rates for C. difficile reduced by 77% (2.398 to 0.549 cases/1000 pt-bds), for MRSA by 25% (1.187 to 0.894 cases/1000 pt-bds) and for ESBL-producing coliforms by 17% (1.480 to 1.224 cases/1000 pt-bds). Time-lag modelling confirmed significant associations between ceftriaxone and C. difficile cases at 1 month (correlation 0.83; P<0.005), and between ciprofloxacin and ESBL-producing coliform cases at 2 months (correlation 0.649; P=0.002). An audit performed 3 years after the policy showed sustained reduction in C. difficile rates (0.259 cases/1000 pt-bds), with additional decreases for MRSA (0.409 cases/1000 pt-bds) and ESBL-producing coliforms (0.809 cases/1000 pt-bds). In conclusion, banning two antibiotics resulted in an immediate and profound reduction in hospital-acquired C. difficile, with possible longer-term effects on MRSA and ESBL-producing coliform rates. Antibiotic stewardship is fundamental in the control of major hospital pathogens. PMID:23276500

  13. Theory of Multiple Tube Fermentation Detection of Coliform Bacteria in Drinking Water%论多管发酵法检测生活饮用水中大肠菌群

    崔丽琳

    2015-01-01

    Objective To study multiple tube fermentation method to detect coliforms in drinking water,the coliform contamination situation of preliminary judging. Methods 80 water samples taken from drinking water including wel water 25 parts,35 parts of the reservoir water,20 parts of urban water supply,the use of multiple tube fermentation method to detect coliform bacteria in drinking water analyzes coliform contamination. Results In the 80 water samples ,the overal pass rate of 87.5%,wel there are 25 parts, a pass rate is 88 percent ,35 parts water reservoir coliform bacteria ,a pass rate is 85.71 percent ,20 parts of the town ,a pass rate of 90%, there were no statistical y significant difference between the rate about three different drinking water passing,P>0.05. Conclusion The pass rate of our daily drinking water needs to be improved.%目的:利用多管发酵法检测生活饮用水中大肠杆菌群情况,对被大肠菌群污染的情况做初步判断。方法采取生活饮用水80份水样,其中包括井水25份,蓄水池水35份,城镇自来水20份,利用多管发酵法检测生活饮用水中大肠菌群,分析大肠杆菌群污染情况。结果在80份水样中,总体合格率为87.5%,井水25份中其合格率为88%,蓄水池水35份的合格率为85.71%,城镇自来水20份的合格率为90%,三种不同生活饮用水合格率之间差异无统计学意义,P>0.05。结论我们日常中的生活饮用水的合格率有待提高,同时要加强水污染管理。

  14. A middle evaluation report on R and D subjects in 2000 fiscal year. Evaluation subject; 'draft of total program on ground disposal research on the high level radioactive wastes'

    The Japan Nuclear Cycle Development Institute (JNC) consulted the titled middle evaluation to the Subject Evaluation Committee (SEC) according to the Schematic indication on practice procedure of evaluation common to the generalized national R and D' and so on. By receiving the consult, SEC on wastes treatment and disposal carried out evaluation of this subject on basis of documents proposed from JNC and discussions at SEC according to an evaluation procedure determined by SEC. The program is to contribute to actualization of safe disposal of the high level radioactive wastes at a technical side. In order to promote the program to practice of the disposal without delay and to transfer its next stage smoothly, it is essential for JNC to prepare an R and D plan after the second summary. From these, this program is precise and adequate in its aim and meaning, high in its importance and agreeable to policy of government and needs of society. Here was summarized by the evaluation results with documents proposed by JNC. As a result of the evaluation, it was shown that as general directionality of this program was judged to be valid, its contents has some places without matching or always clearness with schedule of government and executing groups on practice of disposal. And, it was also shown that some points required for notice on promotion of this program. (G.K.)

  15. Total Synthesis of Teixobactin.

    Giltrap, Andrew M; Dowman, Luke J; Nagalingam, Gayathri; Ochoa, Jessica L; Linington, Roger G; Britton, Warwick J; Payne, Richard J

    2016-06-01

    The first total synthesis of the cyclic depsipeptide natural product teixobactin is described. Synthesis was achieved by solid-phase peptide synthesis, incorporating the unusual l-allo-enduracididine as a suitably protected synthetic cassette and employing a key on-resin esterification and solution-phase macrolactamization. The synthetic natural product was shown to possess potent antibacterial activity against a range of Gram-positive pathogenic bacteria, including a virulent strain of Mycobacterium tuberculosis and methicillin-resistant Staphylococcus aureus (MRSA). PMID:27191730

  16. Total quality accounting

    Andrijašević Maja

    2008-01-01

    Full Text Available The focus of competitive "battle" shifted from the price towards non-price instruments, above all, towards quality that became the key variable for profitability increase and achievement of better comparative position of a company. Under such conditions, management of a company, which, according to the established and certified system of total quality, strives towards achieving of a better market position, faces the problem of quality cost measurement and determination. Management, above all, cost accounting can help in solving of this problem, but the question is how much of its potential is being used for that purpose.

  17. TOTAL annual report 2003

    This 2003 annual report of the Group Total provides economical results and information of the society on the following topics: keys data, the corporate governance (Directors charter, board of directors, audit committee, nomination and remuneration committee, internal control procedures, compensation of directors and executive officers), the corporate social responsibility (environmental stewardship, the future of energy management, the safety enhancement, the human resources, ethics and local development), the investor relations, the management report, the upstream exploration and production, the downstream refining, marketing, trading and shipping, the chemicals and financial and legal information. (A.L.B.)

  18. Total Factbook 2003

    This report presents the activities and results of the Group Total-Fina-Elf for the year 2003. It brings information and economic data on the following topics: the corporate and business; the upstream activities with the reserves, the costs, standardized measure and changes of discounted future net cash flow,oil and gas acreage, drilling, liquefied natural gas, pipelines; downstream activities with refining and marketing maps, refinery, petroleum products, sales, retail gasoline outlets; chemicals with sales and operating income by sector, major applications, base chemicals and polymers, intermediates and performance polymers. (A.L.B.)

  19. Total 2004 fact book

    This report presents the activities and results of the Group Total-Fina-Elf for the year 2004. It brings information and economic data on the following topics: the corporate and business; the upstream activities with the reserves, the costs, standardized measure and changes of discounted future net cash flow,oil and gas acreage, drilling, liquefied natural gas, pipelines; downstream activities with refining and marketing maps, refinery, petroleum products, sales, retail gasoline outlets; chemicals with sales and operating income by sector, major applications, base chemicals and polymers, intermediates and performance polymers. (A.L.B.)

  20. Total quality is people

    Confronted by changing market conditions and increased global competition, in 1983 the Commercial Nuclear Fuel Division (CNFD) of Westinghouse Electric embarked on an ambitious plan to make total quality the centerpiece of its long-term business strategy. Five years later, the division's efforts in making continuous quality improvement a way of life among its more than 2,000 employees gained national recognition when it was named a charter recipient of the Malcolm Baldridge National Quality Award. What CNFD achieved during the 1980s was a cultural transformation, characterized by an empowered work force committed to a common vision. The company's quality program development strategy is described