targeting integrin-linked kinase: Topics by WorldWideScience.org

Development of small-molecule inhibitors of the group I p21-activated kinases, emerging therapeutic targets in cancer

We have studied the impact of overexpression of an intracellular signaling protein, integrin-linked kinase (ILK), on the survival and function of encapsulated islet tissue used for the treatment of type 1 diabetes. The dimensions of the encapsulated tissue can impact the stresses placed on the tissue and ILK overexpression shows the ability to extend function of dissociated cells as well as intact islets. These results suggest that lost cell-extracellular matrix interactions in cell encapsulation systems can lead to decreased insulin secretion and ILK signaling is a target to overcome this phenomenon. (communication)

2010-12-15

2

Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation

The p21-activated kinases (PAKs), immediate downstream effectors of the small G-proteins of the Rac/cdc42 family, are critical mediators of signaling pathways regulating cellular behaviors and...Full Text Available

2010-09-01

3

Predictive and prognostic markers for epidermal growth factor receptor inhibitor therapy in non-small cell lung cancer

Target of rapamycin (TOR) kinases control cell growth through two functionally distinct multiprotein complexes. TOR complex 1 (TORC1) controls temporal cell growth and is sensitive to rapamycin, whereas...Full Text Available

2008-09-23

4

Adverse cutaneous reactions secondary to tyrosine kinase inhibitors including imatinib mesylate, nilotinib, and dasatinib

Epidermal growth factor receptor (EGFR) related therapies – mainly tyrosine kinase inhibitors (TKIs) such as erlotinib and gefitinib, but also monoclonal antibodies targeting EGFR, for example,...Full Text Available

2009-11-01

5

British Library Electronic Table of Contents (United Kingdom)

Abstract Imatinib mesylate is the first of a novel group of drugs that specifically target protein tyrosine kinases, which are central to the pathogenesis of human cancer. It has been approved for the treatment of chronic myeloid leukemia and gastrointestinal stromal tumor and has been found efficacious in other neoplastic diseases. Nilotinib and dasatinib, a second-generation of tyrosine kinase inhibitors (TKIs), were developed in response to findings of emerging imatinib resistance or intolerance to the drug. Cutaneous reactions are the most common nonhematologic side effect of these drugs, and their management is challenging especially in the absence of alternative anticancer agents. The present review focuses on the clinical characteristics and the hypothesized molecular pathogenesis o...

2011-01-01

6

Targeting AMP-activated protein kinase in adipocytes to modulate obesity-related adipokine production associated with insulin resistance and breast cancer cell proliferation

Dietary turmeric modulates DMBA-induced p21ras, MAP kinases and AP-1/NF-?B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

BackgroundAdipokines, e.g. TNFα, IL-6 and leptin increase insulin resistance, and consequent hyperinsulinaemia influences breast cancer progression. Beside its mitogenic...Full Text Available

7

British Library Electronic Table of Contents (United Kingdom)

The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12?weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished ...

2008-01-01

8

The ATG1/ATG13 Protein Kinase Complex Is Both a Regulator and a Target of Autophagic Recycling in Arabidopsis.

A modified immunoblot method to identify substrates of protein kinases

Autophagy is an intracellular recycling route in eukaryotes whereby organelles and cytoplasm are sequestered in vesicles, which are subsequently delivered to the vacuole for breakdown. The process is induced by various nutrient-responsive signaling cascades converging on the Autophagy-Related1 (ATG1)/ATG13 kinase complex. Here, we describe the ATG1/13 complex in Arabidopsis thaliana and show that it is both a regulator and a target of autophagy. Plants missing ATG13 are hypersensitive to nutrient limitations and senesce prematurely similar to mutants lacking other components of the ATG system. Synthesis of the ATG12-ATG5 and ATG8-phosphatidylethanolamine adducts, which are essential for autophagy, still occurs in ATG13-deficient plants, but the biogenesis of ATG8-decorated autophagic bodies does not, indicating that the complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Surprisingly, levels of the ...

2011-10-01

9

Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance

Full Text Available

10

British Library Electronic Table of Contents (United Kingdom)

Aims A depressed cGMP-dependent protein kinase (PKG) activity is implicated in nitrate tolerance. The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance. Methods and results Nitrate tolerance in in vitro preparations was obtained by a 24 h incubation with nitroglycerin (NTG). Nitrate tolerance in in vivo preparations was obtained by subcutaneous injection of mice with NTG, and the aortas were used. Protein levels of total MYPT1, MYPT1 (LZ+), PP1Cd, myosin light chain (MLC), and phosphorylated MLC were determined by Western blot analysis. Isometric vessel tension was determined by an organ chamber technique. Protein levels of MYPT1...

2010-01-01

11

ERK-dependent and -independent pathways trigger human neural progenitor cell migration

Nuclear Targeting of Methyl-Recycling Enzymes in Arabidopsis thaliana Is Mediated by Specific Protein Interactions.

Besides differentiation and apoptosis, cell migration is a basic process in brain development in which neural cells migrate several centimeters within the developing brain before reaching their proper positions and forming the right connections. For identifying signaling events that control neural migration and are therefore potential targets of chemicals to disturb normal brain development, we developed a human neurosphere-based migration assay based on normal human neural progenitor (NHNP) cells, in which the distance is measured that cells wander over time. Applying this assay, we investigated the role of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) in the regulation of NHNP cell migration. Exposure to model substances like ethanol or phorbol 12-myristate 13-acetate (PMA) revealed a correlation between ERK1/2 activation and cell migration. The participation of phospho-(P-) ERK1/2 was confirmed by exposure of the cells to the ...

2007-05-15

12

Rapamycin (sirolimus) protects against hypoxic damage in primary heart cultures via Na^+/Ca^2^+ exchanger activation

Numerous transmethylation reactions are required for normal plant growth and development. S-adenosylhomocysteine hydrolase (SAHH) and adenosine kinase (ADK) act coordinately to recycle the by-product of these reactions, S-adenosylhomocysteine (SAH) that would otherwise competitively inhibit methyltransferase (MT) activities. Here, we report on investigations to understand how the SAH produced in the nucleus is metabolized by SAHH and ADK. Localization analyses using green fluorescent fusion proteins demonstrated that both enzymes are capable of localizing to the cytoplasm and the nucleus, although no obvious nuclear localization signal was found in their sequences. Deletion analysis revealed that a 41-amino-acid segment of SAHH (Gly(150)-Lys(190)) is required for nuclear targeting of this enzyme. This segment is surface exposed, shows unique sequence conservation patterns in plant SAHHs, and possesses additional features of protein-protein ...

2011-10-01

13

British Library Electronic Table of Contents (United Kingdom)

Aims: Rapamycin (sirolimus) is an antibiotic that inhibits protein synthesis through mammalian targeting of rapamycin (mTOR) signaling, and is used as an immunosuppressant in the treatment of organ rejection in transplant recipients. Rapamycin confers preconditioning-like protection against ischemic-reperfusion injury in isolated mouse heart cultures. Our aim was to further define the role of rapamycin in intracellular Ca^2^+ homeostasis and to investigate the mechanism by which rapamycin protects cardiomyocytes from hypoxic damage. Main methods: We demonstrate here that rapamycin protects rat heart cultures from hypoxic-reoxygenation (H/R) damage, as revealed by assays of lactate dehydrogenase (LDH) and creatine kinase (CK) leakage to the medium, by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-d...

2011-01-01

14

Src Kinase Inhibition Decreases Thrombin Induced Injury and Cell Cycle Re-Entry in Striatal Neurons

Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer

Since Src kinase inhibitors decrease brain injury produced by intracerebral hemorrhage (ICH) and thrombin is activated following ICH, this study determined whether Src kinase inhibitors decrease...Full Text Available

2008-05-01

15

Epidermal growth factor receptor (EGFR) kinase domain mutations hyperactivate the kinase and confer kinase addiction of the non-small-cell lung cancer (NSCLC) tumor...Full Text Available

16

EhMAPK, the Mitogen-Activated Protein Kinase from Entamoeba histolytica Is Associated with Cell Survival

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this...Full Text Available

1994-05-24

17

Cardiac Myosin Is a Substrate for Zipper-interacting Protein Kinase (ZIPK)*

Mitogen Activated Protein Kinases (MAPKs) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and...Full Text Available

18

Activation of fat cell adenylate cyclase by protein kinase C

Zipper-interacting protein kinase (ZIPK) is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain...Full Text Available

2010-02-19

19

AMP-Activated Protein Kinase: A Stress-Responsive Kinase with Implications for Cardiovascular Disease

Purified protein kinase C (C-kinase) from guinea pig pancreas and rat brain stimulated adenylate cyclase activity in purified rat adipocyte membranes. Cyclase stimulation occurred over 100 to 1000 mU/ml of C-kinase activity, required greater than 10 ..mu..M calcium, proceeded without a lag, was not readily reversible, and required no exogenous phospholipid. Moreover, C-kinase inhibitors, such as chlorpromazine and palmitoyl carnitine, inhibited selectively adenylate cyclase which was activated by C-kinase and calcium. Depending on assay conditions, 10 nM 12-0-tetradecanoylphorbol-13-acetate (TPA) either enhanced or was required for kinase action on cyclase. Also, TPA plus calcium promoted the quantitative association of C-kinase with membranes. Adenylate cyclase activation by C-kinase was seen both in the presence and ...

1986-05-01

20

Phosphorylation of Annexin A1 by TRPM7 Kinase: A Switch Regulating the Induction of an ?-Helix

AMP-activated protein kinase (AMPK) was initially viewed as energy sensor and activated by increased intracellular concentrations of AMP following nutrient deprivation. Physiological or pathological...Full Text Available

2010-04-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

21

Influence of valency and labelling chemistry on in vivo targeting using radioiodinated HER2-binding Affibody molecules

TRPM7 is an unusual bifunctional protein consisting of an α-kinase...Full Text Available

2011-03-29

22

British Library Electronic Table of Contents (United Kingdom)

Purpose HER2 is a transmembrane tyrosine kinase, which is overexpressed in a number of carcinomas. The Affibody molecule ZHER2:342 is a small (7?kDa) affinity protein binding to HER2 with an affinity of 22?pM. The goal of this study was to evaluate the use of ((4-hydroxyphenyl)ethyl)maleimide (HPEM) for radioiodination of ZHER2:342 and to compare the targeting properties of monomeric and dimeric forms of ZHER2:342. Methods The biodistribution of different radioiodinated derivatives of ZHER2:342 was studied in BALB/C nu/nu mice bearing HER2-expressing SKOV-3 xenografts. Biodistributions of 125I-PIB-ZHER2:342 and site-specifically labelled 125I-HPEM-ZHER2:342-C were compared. Biodistributions of monomeric 131I-HPEM-ZHER2:342-C and dimeric 125I-HPEM-(ZHER2:342)2-C were evaluated using a paire...

2009-01-01

23

Structure and Function of NtCDPK1, a Calcium-dependent Protein Kinase in Tobccco

Gamma interferon induces rapid and coordinate activation of mitogen-activated protein kinase (extracellular signal-regulated kinase) and calcium-independent protein kinase C in human monocytes.

Full Text Available

2000-05-01

24

Crystal structures of the N-terminal kinase domain of human RSK1 bound to three different ligands: Implications for the design of RSK1 specific inhibitors

Gamma interferon plays an important role in regulating the functional properties of mononuclear phagocytes. In the present study, the role of activated protein kinases in the mechanism of action of...Full Text Available

1994-07-01

25

Phosphorylation of sites 3 and 2 in rabbit skeletal muscle glycogen synthase by a multifunctional protein kinase (ATP-citrate lyase kinase)

The p90 ribosomal S6 kinases (RSKs) also known as MAPKAP-Ks are serine/threonine protein kinases that are activated by ERK or PDK1 and act as downstream effectors of mitogen-activated protein kinase...Full Text Available

2007-12-01

26

Differential regulation of the biosynthesis of glucose transporters by the PI3-K and MAPK pathways of insulin signaling by treatment with novel compounds from Liriope platyphylla.

A multifunctional protein kinase, purified from rat liver as ATP-citrate lyase kinase, has been identified as a glycogen synthase kinase. This kinase catalyzed incorporation of up to 1.5 mol of and)2numberSPO4/mol of synthase subunit associated with a decrease in the glycogen synthase activity ratio from 0.85 to a value of 0.15. Approximately 65-70% of the TUPO4 was incorporated into site 3 and 30-35% into site 2 as determined by reverse phase high performance liquid chromatography. This multifunctional kinase was distinguished from glycogen synthase kinase-3 on the basis of nucleotide and protein substrate specificities. Since the phosphate contents in glycogen synthase of sites 3 and 2 are altered in diabetes and by insulin administration, the possible involvement of the multifunctional kinase was explored. Glycogen synthase purified from ...

1985-10-05

27

Insulin-induced decrease in protein phosphorylation in rat adipocytes not explained by decreased A-kinase activity

The insulin signaling pathway, involving protein kinase B (PKB) and mitogen-activated protein kinase (MAPK), mediates the biological response to insulin and several growth factors and cytokines. To investigate the correlation between glucose transporter (Glut) biosynthesis and the insulin signaling pathway activated by novel compounds of Liriope platyphylla (LP9M80-H), alterations in Glut and key protein expression in the insulin signaling pathway were analyzed in the liver and brain of ICR mice treated with LP9M80-H. An in vitro assay showed that the highest level of insulin concentration was observed in the LP9M80-H-treated group, followed by the LP-H, LP-M, LP-E, and LP9M80-C-treated groups. Therefore, LP9M80-H was selected for use in studying the detailed mechanism of the insulin signaling pathway in animal systems. In an in vivo experiment, LP9M80-H induced a significant increase in glucose levels and a decrease of insulin concentration in ...

2010-12-14

28

Final report of the specific research. Investigations on the analysis of bio-protective factors against radiation. 1998-2000 FY (Research Group of NIRS)

In isolated rat adipocytes, insulin inhibits lipolysis to a greater extent than would be predicted by the decrease in (-/+)cAMP activity ratio of cAMP-dependent protein kinase (A-kinase), from which it was speculated that insulin promotes the dephosphorylation of hormone-sensitive lipase. They have examined the phosphorylation state of cellular proteins under conditions of varying A-kinase activities in the presence and absence of insulin. Protein phosphorylation was determined by SDS-PAGE electrophoresis of extracts from /sup 32/P-loaded cells; glycerol and A-kinase activity ratios were measured in the cytosolic extracts from control, non-radioactive cells. Increased protein phosphorylation in general occurred over the same range of A-kinase activity ratios, 0.1-0.3, associated with increased glycerol release. The insulin-induced decrease in lipolysis was associated with a decrease ...

1987-05-01

29

Development of a novel mouse tk{sup +/-} embryonic stem cell line for use in mutagenicity studies

This report concerns investigations in the title conducted by 8 groups of National Institute of Radiological Sciences (NIRS) during the period of 1998-2000. The groups are for investigation of: Effects of p53 tumor suppressor gene in radiation-induced leukemia, Role of atm-gene in dose rate effect of ionizing radiation, Function of DNA-dependent protein kinase catalytic subunit (DNA-PK{sub cs}), Functional complementation of radiation-sensitive mutant M10 cell line by human XRCC4 cDNA expression, Role of radiation-induced apoptosis in digital defects in embryonic mice, Functional analysis of S-phase specific novel nuclear protein NP95 by gene targeting, Role of chemokine in T cell development and lymphomagenesis, and establishment of production techniques of gene-modified mice using embryonic stem cells for genetic analysis of radiation-sensitive genes. The groups describe summaries of their studies and published original articles are also ...

2002-03-01

30

The impact of a repeated bout of eccentric exercise on muscular strength, muscle soreness and creatine kinase.

A tk{sup +/-} mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk{sup +/-} L5178Y cell line, which is used widely to assess the mutagenicity of chemical agents. Treatment of 1G2 cells with the alkylating agent N-ethyl-N-nitrosourea (ENU) resulted in a dose-related increase in tribluorothymidine-resistant colonies. Mutant frequencies of 152 and 296 per 10{sup 6} cells were determined for 0.1 and 0.3 mg/ml doses of ENU, compared with a spontaneous mutant frequency of 15 per 10{sup 6} cells. The data indicate that tk{sup +/-} 1G2 ES cells may be useful for the creation of a transgenic mouse model for assessing in vivo mutation using an endogenous autosomal gene. 45 refs., 2 figs., 1 tab.

1996-12-31

31

Syk Tyrosine Kinase Acts as a Pancreatic Adenocarcinoma Tumor Suppressor by Regulating Cellular Growth and Invasion

The purpose of this study was to determine if there were any beneficial or detrimental effects regarding delayed onset muscle soreness (DOMS), serum creatine kinase (CK), and maximum concentric strength...Full Text Available

1994-12-01

32

Separation, Purification, and Comparative Properties of Chloroplast and Cytoplasmic Phosphoglycerate Kinase from Barley Leaves ¹

We have identified the nonreceptor tyrosine kinase syk as a marker of differentiation/tumor suppressor in pancreatic ductal adenocarcinoma (PDAC). Syk expression is lost in poorly differentiated PDAC...Full Text Available

2009-12-01

33

Phosphoglycerate kinase and triosephosphate isomerase from the hyperthermophilic bacterium Thermotoga maritima form a covalent bifunctional enzyme complex.

The chloroplast and cytoplasmic isoenzymes of phosphoglycerate kinase (PGK) (EC. 2.7.2.3) from Hordeum vulgare leaves have been separated and purified for the first time to apparent...Full Text Available

1990-06-01

34

P-glycoprotein and breast cancer resistance protein in acute myeloid leukaemia cells treated with the Aurora-B Kinase Inhibitor barasertib-hQPA

Phosphoglycerate kinase (PGK) from the hyperthermophilic bacterium Thermotoga maritima has been purified to homogeneity. A second larger enzyme with PGK activity and identical N-terminal sequence was...Full Text Available

1995-02-01

35

Involvement of AMP-activated-protein-kinase (AMPK) in neuronal amyloidogenesis

BackgroundAurora kinases play an essential role in orchestrating chromosome alignment, segregation and cytokinesis during mitotic progression, with both aurora-A and B frequently...Full Text Available

36

Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture

AMP-activated-protein-kinase (AMPK) is a key sensor and regulator of cellular and whole-body energy metabolism and plays a key role in regulation of lipid metabolism. Since lipid metabolism...Full Text Available

2010-09-03

37

Expression and Characterization of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase PknB

During a study on the mTor pathway in the rat kidney we observed a striking increase of the phosphorylation of the S6 kinase in mitosis. In cryostat sections of perfusion-fixed tissue mitotic cells...Full Text Available

2007-02-01

38

EFFECT OF AGE ON THE ROLE OF RHO-KINASE IN SHORT TERM PARTIAL BLADDER OUTLET OBSTRUCTION

PknB is a member of the newly discovered eukaryotic-like protein serine/threonine kinase (PSTK) family of proteins. The pknB gene was cloned and expressed in Escherichia coli....Full Text Available

1999-11-01

39

Deficiency of Phosphoinositide 3-Kinase Enhancer Protects Mice From Diet-Induced Obesity and Insulin Resistance

ObjectivesWe examined the expression of Rho Kinase (ROK) isoforms in young and old rabbit’s detrusor smooth muscles (SM) during the progression of short term...Full Text Available

2008-03-01

40

Chronic inflammation and estradiol interact through MAPK activation to affect TMJ nociceptive processing by trigeminal caudalis neurons

OBJECTIVEPhosphoinositide 3-kinase enhancer A (PIKE-A) is a proto-oncogene that promotes tumor growth and transformation by enhancing Akt activity. However, the physiological functions...Full Text Available

2010-04-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

41

CID755673 enhances mitogenic signaling by phorbol esters, bombesin and EGF through a protein kinase D-independent pathway

The mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway plays a key role in mediating estrogen actions in the brain and neuronal sensitization during inflammation....Full Text Available

2009-12-29

42

Autoantibodies to BRAF, a new family of autoantibodies associated with rheumatoid arthritis

Recently, CID755673 was reported to act as a highly selective inhibitor of protein kinase D (PKD). In the course of experiments using CID755673, we noticed that it exerted unexpected stimulatory...Full Text Available

2010-01-01

43

Antisense Expression of the CK2 ?-Subunit Gene in Arabidopsis. Effects on Light-Regulated Gene Expression and Plant Growth1

IntroductionBRAF (v raf murine sarcoma viral oncogene homologue B1) is a serine-threonine kinase involved in the mitogen-activated protein kinase (MAPK) signalling pathway, known...Full Text Available

2010-01-01

44

Allosteric regulation of glycerol kinase by enzyme IIIglc of the phosphotransferase system in Escherichia coli and Salmonella typhimurium.

The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because...Full Text Available

1999-03-01

45

AMP-activated protein kinase (AMPK) cross-talks with canonical Wnt signaling via phosphorylation of ?-catenin at Ser 552

The mechanism by which enzyme IIIglc of the bacterial phosphotransferase system regulates the activity of crystalline glycerol kinase from Escherichia coli has been studied, and the inhibitory effects...Full Text Available

1985-05-01

46

c-Jun N-terminal Kinase 2 Regulates Multiple Receptor Tyrosine Kinase Pathways in Mouse Mammary Tumor Growth and Metastasis

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism; its activity is regulated by a plethora of physiological conditions, exercises and many anti-diabetic drugs. Recent...Full Text Available

2010-04-23

47

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis^[W]^[OA]

c-Jun N-terminal kinase 2 (JNK2) isoforms are transcribed from the jnk2 gene and are highly homologous with jnk1 and jnk3 transcriptional products....Full Text Available

2011-01-01

48

Canonical and Alternative Pathways in Cyclin-Dependent Kinase 1/Cyclin B Inactivation upon M-Phase Exit in Xenopus laevis Cell-Free Extracts

In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal...Full Text Available

2010-05-01

49

Phospholemman: A Novel Cardiac Stress Protein

Cyclin-Dependent Kinase 1 (CDK1) is the major M-phase kinase known also as the M-phase Promoting Factor or MPF. Studies performed during the last decade have shown many details of how CDK1 is regulated...Full Text Available

50

Cardiac Enzymes, Renal Failure and Renal Transplantation

Phospholemman (PLM), a member of the FXYD family of regulators of ion transport, is a major sarcolemmal substrate for protein kinases A and C in cardiac and skeletal muscle. In the heart, PLM...Full Text Available

2010-08-01

51

Small-molecule screen identifies inhibitors of a human intestinal calcium-activated chloride channel.

Diagnostic accuracy of the currently available serum markers of cardiac injury, such as myoglobin, creatine kinase and its myocardial isoform, are altered in patients with renal failure. It is shown...Full Text Available

2006-03-01

52

Honokiol-mediated inhibition of PI3K/mTOR pathway: a potential strategy to overcome immunoresistance in glioma, breast, and prostate carcinoma without impacting T cell function.

Calcium-activated chloride channels (CaCCs) are widely expressed in mammalian tissues, including intestinal epithelia, where they facilitate fluid secretion. Potent, selective CaCC inhibitors have not been available. We established a high-throughput screen for identification of inhibitors of a human intestinal CaCC based on inhibition of ATP/carbachol-stimulated iodide influx in HT-29 cells after lentiviral infection with the yellow fluorescent halide-sensing protein YFP-H148Q/I152L. Screening of 50,000 diverse, drug-like compounds yielded six classes of putative CaCC inhibitors, two of which, 3-acyl-2-aminothiophenes and 5-aryl-2-aminothiazoles, inhibited by >95% iodide influx in HT-29 cells in response to multiple calcium-elevating agonists, including thapsigargin, without inhibition of calcium elevation, calcium-calmodulin kinase II activation, or cystic fibrosis transmembrane conductance regulator chloride channels. These compounds also inhibited ...

2007-12-14

53

Gene Repressive Activity of RIP140 Through Direct Interaction with CDK8.

Inhibition of the phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway is an appealing method for decreasing the immunoresistance and augmenting T cell-mediated immunotherapy. A major impediment to this strategy is the impact of conventional PI3K/mTOR pathway inhibitors on T cell function. In particular, rapamycin, is a well-known immunosuppressant that can decrease the activity of the PI3K/mTOR pathway in tumor cells, but also has a profound inhibitory effect on T cells. Here we show that Honokiol, a natural dietary product isolated from an extract of seed cones from Magnolia grandiflora, can decrease PI3K/mTOR pathway-mediated immunoresistance of glioma, breast and prostate cancer cell lines, without affecting critical proinflammatory T cell functions. Specifically, we show that at doses sufficient to down-regulate levels of phospho-S6 and the negative immune regulator B7-H1 in tumor cells, Honokiol does not ...

54

Disruption of contact inhibition in rat liver epithelial cells by various types of AhR ligands

Receptor interacting protein 140 (RIP140) is a coregulator for numerous nuclear receptors and transcription factors and primarily exerts gene-repressive activities on various target genes. We previously identified a spectrum of posttranslational modifications on RIP140 that augment its property and biological activity. In T(3)-triggered biphasic regulation of cellular retinoic acid binding protein 1 (Crabp1) gene along the course of fibroblast-adipocyte differentiation, we found TRAP220(MED1) critical for T(3)-activated chromatin remodeling whereas RIP140 essential for T(3)-repressive chromatin remodeling of this gene promoter. In this current study, we aim to examine whether and how RIP140 replaces TRAP220(MED1) on the CrabpI promoter in differentiating adipocyte cultures. We find increasing recruitment of RIP140 to this promoter, with corresponding reduction in TRAP220(MED1) recruitment during the T(3)-repressive phase. We also uncover direct interaction of ...

2011-08-25

55

c-JUN N-TERMINAL KINASE MODULATES OXIDANT STRESS AND PEROXYNITRITE FORMATION INDEPENDENT OF INDUCIBLE NITRIC OXIDE SYNTHASE IN ACETAMINOPHEN HEPATOTOXICITY

The maintenance of a balance between cell gain and cell loss is essential for proper liver function. The exact role of aryl hydrocarbon receptor (AhR) in regulating cell proliferation and apoptosis of liver cells remains unclear, since ligand-dependent activation of AhR has been shown to induce cell cycle arrest, proliferation, differentiation or apoptosis, depending on the cellular model used. AhR can directly interact with retinoblastoma protein in hepatic cells, forming protein complexes that can efficiently block cell cycle progression by inducing G1 arrest, or to induce the expression of inhibitors of cyclin-dependent kinases, such as p271. On the other hand, it has been suggested that AhR could play a stimulatory role in cell proliferation, either directly or by mediating a release from contact inhibition. It is now generally accepted that progenitor cells exist in the liver, are activated in various liver diseases and can form a potential ...

2004-09-15

56

Up-Regulation of the RhoA/Rho-Kinase Signaling Pathway in Corpus Cavernosum from Endothelial Nitric-Oxide Synthase (NOS), but Not Neuronal NOS, Null Mice

Acetaminophen (APAP) overdose, which causes liver injury in animals and humans, activates c-jun N-terminal kinase (JNK). Although it was shown that the JNK inhibitor SP600125 effectively reduced...Full Text Available

2010-07-01

57

Structural features underlying selective inhibition of protein kinase CK2 by ATP site-directed tetrabromo-2-benzotriazole

We tested the hypothesis that the basal release of nitric oxide (NO) from endothelial cells modulates contractile activity in the corpus cavernosum (CC) via inhibition of the RhoA/Rho-kinase signaling...Full Text Available

2010-04-01

58

Selective control of amino acid metabolism by the GCN2 eIF2 kinase pathway in Saccharomyces cerevisiae

Two novel crystal structures of Zea mays protein kinase CK2α catalytic subunit, one in complex with the specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) and another...Full Text Available

2001-11-01

59

Phosphorylation of ?6-Tubulin by Protein Kinase C? Activates Motility of Human Breast Cells*

BackgroundWhen eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. Activated Gcn2p up-regulates the general amino...Full Text Available

60

Kappa-opioid receptor activation during reperfusion limits myocardial infarction via ERK1/2 activation in isolated rat hearts

Engineered overexpression of protein kinase Cα (PKCα) was previously shown to endow nonmotile MCF-10A human breast cells with aggressive motility. A traceable mutant of PKCα...Full Text Available

2009-06-26

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

61

Involvement of stress-activated protein kinase and p38 mitogen-activated protein kinase in mIgM-induced apoptosis of human B lymphocytes

BackgroundWe investigated whether p42/p44 extracellular signal-regulated kinases (ERK1/2) and/or phosphatidylinositol-3-OH kinase (PI3K)-Akt play a crucial role in cardioprotection...Full Text Available

2011-05-01

62

Evolution of a bifunctional enzyme: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Despite intensive efforts, the intracellular signaling pathways that mediate apoptosis remain unclear. The human B lymphoma cell line, B104, possesses characteristics that make it an attractive model...Full Text Available

1996-11-26

63

A New Metal-Binding Site for Yeast Phosphoglycerate Kinase as Determined by the Use of a Metal-ATP Analog

The bifunctional rat liver enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (ATP:D-fructose-6-phosphate 2-phosphotransferase/D-fructose-2,6-bisphosphate 2-phosphohydrolase, EC 2.7.1.105/EC...Full Text Available

1989-12-01

64

(-)-Epigallocatechin Gallate Regulates CD3-mediated T Cell Receptor Signaling in Leukemia through the Inhibition of ZAP-70 Kinase*

Suicide substrate β, γ-bidentate Rh(III)ATP (RhATP) was used to map the metal ion-binding site in yeast phosphoglycerate kinase (PGK). Cleavage of the RhATP-inactivated enzyme with...Full Text Available

1997-02-01

65

Targeting vaccines to dendritic cells

The ζ chain-associated 70-kDa protein (ZAP-70) of tyrosine kinase plays a critical role in T cell receptor-mediated signal transduction and the immune response. A high level of ZAP-70 expression...Full Text Available

2008-10-17

66

Constraints on target chamber first wall and target designs that will enable NIF debris shields to survive

Udgivelsesdato: 2002-Mar

2002-01-01

67

A shockproof container for the transport and storage of fragile or reactive target foils

Constraints on target chamber first wall and target designs that will enable NIF debris shields to survive

1999-01-01

68

The hippo pathway in biological control and cancer development

A shockproof container for the transport and storage of fragile or reactive target foils

1971-01-01

69

British Library Electronic Table of Contents (United Kingdom)

Abstract The Hippo pathway is an evolutionally conserved protein kinase cascade involved in regulating organ size in vivo and cell contact inhibition in vitro by governing cell proliferation and apoptosis. Deregulation of the Hippo pathway is linked to cancer development. Its first core kinase Warts was identified in Drosophila more than 15 years ago, but it gained much attention when other core components of the pathway were identified 8 years later. Major discoveries of the pathway were made during past several years. The core kinase components Hippo, Salvador, Warts, and Mats in the fly and Mst1/2, WW45, Lats1/2, and Mob1 in mammals phosphorylate and inactivate downstream transcriptional co-activators Yorkie in the fly, Yes-associated protein (YAP) and transcriptional co-activator with ...

2011-01-01

70

Phorbol esters, protein kinase C, and thyroxine 5 prime -deiodinase in brown adipocytes

Inhibition of the heterotetrameric K++ channel KCNQ1/KCNE1 by the AMP-activated protein kinase

Protein kinase C activity has been identified in the rat brown adipocyte. About 60% of this activity is found in the cytosolic fraction under basal conditions, and 12-O-tetradecanoylphorbol 13-acetate (TPA) causes a rapid shift from the cytosol to the particulate fraction. Norepinephrine and phenylephrine causes a similar redistribution that can be blocked by prazosin but not by alprenolol. {alpha}{sub 1}-Adrenergic agonists cause three- to fivefold stimulation of type 2 iodothyronine 5{prime}-deiodinase activity in brown adipocytes. TPA has no effect on basal deiodinase activity and reduces the response of the enzyme to {alpha}{sub 1}-adrenergic agonists. These results suggest that the translocation of protein kinase C from cytosol to particulate fraction is not sufficient to increase deiodinase activity but can modulate the {alpha}{sub 1}-adrenergic agonist-mediated responses in these cells.

1988-03-01

71

British Library Electronic Table of Contents (United Kingdom)

Abstract The heterotetrameric K++-channel KCNQ1/KCNE1 is expressed in heart, skeletal muscle, liver and several epithelia including the renal proximal tubule. In the heart, it contributes to the repolarization of cardiomyocytes. The repolarization is impaired in ischemia. Ischemia stimulates the AMP-activated protein kinase (AMPK), a serine/threonine kinase, sensing energy depletion and stimulating several cellular mechanisms to enhance energy production and to limit energy utilization. AMPK has previously been shown to downregulate the epithelial Na++ channel ENaC, an effect mediated by the ubiquitin ligase Nedd4-2. The present study explored whether AMPK regulates KCNQ1/KCNE1. To this end, cRNA encoding KCNQ1/KCNE1 was injected into Xenopus oocytes with and without additional injection o...

2011-01-01

72

skittles, a Drosophila phosphatidylinositol 4-phosphate 5-kinase, is required for cell viability, germline development and bristle morphology, but not for neurotransmitter release.

Use of clopidogrel in the reduction of myocardial damage during percutaneous coronary intervention

The phosphatidylinositol pathway is implicated in the regulation of numerous cellular functions and responses to extracellular signals. An important branching point in the pathway is the phosphorylation...Full Text Available

1998-12-01

73

The Receptor Tyrosine Kinase FGFR4 Negatively Regulates NF-kappaB Signaling

It is estimated that approximately a quarter of patients undergoing coronary intervention may have significant post-procedural creatinine (CK)/creatinine kinase myocardial band (CK-MB) elevations and...Full Text Available

2009-01-01

74

The Dynamical Mechanism of Auto-Inhibition of AMP-Activated Protein Kinase

BackgroundNFκB signaling is of paramount importance in the regulation of apoptosis, proliferation, and inflammatory responses during human development and homeostasis, as...Full Text Available

75

The Cytokinin Receptors of Arabidopsis Are Located Mainly to the Endoplasmic Reticulum¹^[W]^[OA]

We use a novel normal mode analysis of an elastic network model drawn from configurations generated during microsecond all-atom molecular dynamics simulations to analyze the mechanism of auto-inhibition...Full Text Available

2011-07-01

76

Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis

The plant hormone cytokinin is perceived by membrane-located sensor histidine kinases. Arabidopsis (Arabidopsis thaliana) possesses three cytokinin receptors: ARABIDOPSIS HISTIDINE...Full Text Available

2011-08-01

77

Role of bile salt in regulating Mcl-1 phosphorylation and chemoresistance in hepatocellular carcinoma cells

BackgroundThe Streptococcus pyogenes (group A streptococci, GAS) two-component signal transduction system CovRS has been described to be important for pathogenesis...Full Text Available

78

Regulation of hyaluronan secretion into rabbit synovial joints in vivo by protein kinase C

BackgroundGlycochenodeoxycholate (GCDA) is one of the major human bile salts. Bile salts stimulate cell survival and proliferation through the mitogen-activated protein kinase, but...Full Text Available

79

Regulation of ROMK1 Channels by Protein-tyrosine Kinase and -tyrosine Phosphatase*

Hyaluronan (HA) is important for joint cavitation, lubrication, volume regulation and synovial fluid drainage but little is known about the regulation of joint HA synthesis/secretion in vivo....Full Text Available

2003-07-15

80

Regulation of Mixed Lineage Kinase 3 is Required for Neurofibromatosis-2-Mediated Growth Suppression in Human Cancer

We have used the two-electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK1 channels by protein-tyrosine phosphatase (PTP) and protein-tyrosine...Full Text Available

2001-03-09

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

81

PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast

The Neurofibromatosis-2 (NF2) tumor suppressor merlin negatively regulates cell proliferation in numerous cell types. We have previously shown that the NF2...Full Text Available

2011-02-17

82

PKC expression is regulated by dietary K intake and mediates internalization of SK channels in the CCD

Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through a conserved system called autophagy. The key process in...Full Text Available

2011-01-01

83

Oligomycin-induced Bioenergetic Adaptation in Cancer Cells with Heterogeneous Bioenergetic Organization

We have used Western blot analysis and immunocytochemistry to determine the effect of dietary K intake on the expression of protein kinase C (PKC) isoforms in the kidney. Western blot has demonstrated...Full Text Available

2004-06-01

84

Neutral endopeptidase inhibits prostate cancer cell migration by blocking focal adhesion kinase signaling

Cancer cells constantly adapt to oxidative phosphorylation (OXPHOS) suppression resulting from hypoxia or mitochondria defects. Under the OXPHOS suppression, AMP-activated protein kinase (AMPK) regulates...Full Text Available

2010-04-23

85

Neurotensin Receptor 1 Is Expressed in Gastrointestinal Stromal Tumors but Not in Interstitial Cells of Cajal

Neutral endopeptidase 24.11 (NEP, CD10) is a cell-surface enzyme expressed by prostatic epithelial cells that cleaves and inactivates neuropeptides implicated in the growth of androgen-independent prostate...Full Text Available

2000-12-01

86

Lnk constrains myeloproliferative diseases in mice

Gastrointestinal stromal tumors (GIST) are thought to derive from the interstitial cells of Cajal (ICC) or an ICC precursor. Oncogenic mutations of the KIT or PDGFRA receptor tyrosine kinases are present...Full Text Available

87

Light-Dependent Regulation of Cyanobacterial Phytochrome Expression

Hematopoietic stem and progenitor cell (HSPC) expansion is regulated by intrinsic signaling pathways activated by cytokines. The intracellular kinase JAK2 plays an essential role in cytokine signaling,...Full Text Available

2010-06-01

88

H-1152 Effects on Intraocular Pressure and Trabecular Meshwork Morphology of Rat Eyes

A histidine kinase protein (Cph1) with sequence homology and spectral characteristics very similar to those of the plant phytochrome has been recently identified in the cyanobacterium Synechocystis...Full Text Available

2000-01-01

89

Elevated c-Src tyrosine kinase activity in premalignant epithelia of ulcerative colitis.

AbstractPurposeThe aim of this study was to elucidate the effects of the Rho-kinase inhibitor, H-1152, on cultured human trabecular meshwork (HTM) cells, TM morphology,...Full Text Available

2008-08-01

90

Effects of norepinephrine infusion on myocardial high-energy phosphate content and turnover in the living rat.

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon with a high incidence of colon cancer. Dysplasia is a precursor to carcinoma and a predictor of malignant potential; epithelia...Full Text Available

1994-02-01

91

DosT and DevS are oxygen-switched kinases in Mycobacterium tuberculosis

Using 31P-nuclear magnetic resonance, we studied the relationship between myocardial high-energy phosphate content and flux values for the creatine kinase reaction in the living rat under inotropic...Full Text Available

1987-06-01

92

Differential Regulation of Mitogen-Activated Protein Kinase Pathways by Acetaminophen and Its Nonhepatotoxic Regioisomer 3?-Hydroxyacetanilide in TAMH Cells

Exposure of Mycobacterium tuberculosis to hypoxia is known to alter the expression of many genes, including ones thought to be involved in latency, via the transcription factor DevR...Full Text Available

2007-08-01

93

DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase

Acetaminophen (APAP), a widely used analgesic and antipyretic that is considered to be relatively safe at recommended doses, is the leading cause of drug-induced liver failure in the United States....Full Text Available

2010-07-01

94

Cytokinin-Dependent Photorespiration and the Protection of Photosynthesis during Water Deficit1[W][OA

CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA...Full Text Available

1999-03-16

95

Co-Induction of LTP and LTD and Its Regulation by Protein Kinases and Phosphatases

We investigated the effects of P_SARK∷IPT (for Senescence-Associated Receptor Kinase∷Isopentenyltransferase) expression and cytokinin production...Full Text Available

2009-07-01

96

Chemical activation of MEK1 - a redox trigger for evaluating the effects of phosphorylation.

The cellular properties of long-term potentiation (LTP) following pairing of pre- and postsynaptic activity were examined at a known glutamatergic synapse in the leech, specifically between the pressure...Full Text Available

2010-05-01

97

Calcium/Calmodulin-Dependent Protein Kinase II Mediates Hippocampal Glutamatergic Plasticity During Benzodiazepine Withdrawal

An approach to generate mimics of phosphorylated serine proteins chemically through site-specific sulfonation of cysteine is presented. This chemical modification is reversible in the presence of reducing agent and therefore is analogous to the kinase/phosphatase system used in nature. PMID:21717004

2011-06-30

98

Beyond AICA Riboside: In Search of New Specific AMP-activated Protein Kinase Activators

Benzodiazepine withdrawal anxiety is associated with potentiation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (AMPAR) currents in hippocampal CA1 pyramidal...Full Text Available

2010-08-01

99

Autophosphorylation Within the Atg1 Activation Loop Is Required for Both Kinase Activity and the Induction of Autophagy in Saccharomyces cerevisiae

Summary5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICA riboside) has been extensively used in vitro and in vivo to...Full Text Available

2009-01-01

100

ATM-Dependent Phosphorylation of ATF2 Is Required for the DNA Damage Response

Autophagy is an evolutionarily conserved degradative pathway that has been implicated in a number of physiological events important for human health. This process was originally identified as a response...Full Text Available

2010-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

101

AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model

SummaryActivating transcription factor 2 (ATF2) is regulated by JNK/p38 in response to stress. Here, we demonstrate that the protein kinase ATM phosphorylates ATF2 on serines...Full Text Available

2005-05-27

102

A Conserved Role for Phosphoinositide-3-Kinase but Not Akt Signaling in Mitochondrial Adaptations that Accompany Physiological Cardiac Hypertrophy

BackgroundObesity is one of the principal causative factors involved in the development of metabolic syndrome. AMP-activated protein kinase (AMPK) is an energy sensor that regulates...Full Text Available

103

?Activation of the mitogen-activated protein kinase pathways by heat shock

Physiological cardiac hypertrophy is associated with mitochondrial adaptations that are characterized by activation of PGC-1α and increased fatty acid oxidative (FAO) capacity. It is...Full Text Available

2007-10-01

104

Functions of mammalian Cdc7 kinase in initiation/monitoring of DNA replication and development

In addition to inducing new transcriptional activities that lead within a few hours to the accumulation of heat shock proteins (Hsps), heat shock activates within minutes the major signaling transduction...Full Text Available

2002-04-01

105

X-ray production yield in standardized thick target PIXE

Cdc7 kinase plays an essential role in firing of replication origins by phosphorylating components of the replication complexes. Cdc7 kinase has also been implicated in S phase checkpoint signaling downstream of the ATR and Chk1 kinases. Inactivation of Cdc7 in yeast results in arrest of cell growth with 1C DNA content after completion of the ongoing DNA replication. In contrast, conditional inactivation of Cdc7 in undifferentiated mouse embryonic stem (ES) cells leads to growth arrest with rapid cessation of DNA synthesis, suggesting requirement of Cdc7 functions for continuation of ongoing DNA synthesis. Furthermore, loss of Cdc7 function induces recombinational repair (nuclear Rad51 foci) and G2/M checkpoint responses (inhibition of Cdc2 kinase). Eventually, p53 becomes highly activated and the cells undergo massive p53-dependent apoptosis. Thus, defective origin activation in mammalian cells can ...

2003-11-27

106

TARGET SIGNAL DETECTOR AND DETECTING METHOD

No abstract prepared.

2003-07-01

107

J-STORE (Japan)

Full Text Available

2005-12-02

108

Prospects for polarized target materials with pure carbon background.

Computational Challenges in miRNA Target Predictions: To Be or Not to Be a True Target?

No abstract available.

1992-01-01

109

APPARATUS OF MAKING UP MEASUREMENT TARGET

All microRNA (miRNA) target—finder algorithms return lists of candidate target genes. How valid is that output in a biological setting? Transcriptome analysis has proven to be a useful approach...Full Text Available

2009-01-01

110

J-STORE (Japan)

Full Text Available

2007-10-26

111

Targeting Prostate Cancer Cells In Vivo Using a Rapidly Internalizing Novel Human Single-Chain Antibody Fragment

Mean energies of photoneutron spectra for sup(67,70)Zn, sup(74,77)Se, sup(84,86,87,88)Sr, sup(181)Ta and sup(183)W isotopes

Human antibodies targeting prostate cancer cell surface epitopes may be useful for imaging and therapy. The objective of this study was to evaluate the tumor targeting of an internalizing human...Full Text Available

2010-03-01

112

Isoform-level microRNA-155 target prediction using RNA-seq

... reactions selenium 74 target selenium 77 target strontium 84 target strontium

1986-04-15

113

Delineating Bacteriostatic and Bactericidal Targets in Mycobacteria Using IPTG Inducible Antisense Expression

Computational prediction of microRNA targets remains a challenging problem. The existing rule-based, data-driven and expression profiling approaches to target prediction are mostly approached from the...Full Text Available

2011-05-01

114

Tomato 14-3-3 Protein 7 Positively Regulates Immunity-Associated Programmed Cell Death by Enhancing Protein Abundance and Signaling Ability of MAPKKK ?^[C]^[W]

In order to identify novel high value antibacterial targets it is desirable to delineate whether the inactivation of the target enzyme will lead to bacterial death or stasis. This knowledge is particularly...Full Text Available

115

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

Programmed cell death (PCD) is triggered when Pto, a Ser-Thr protein kinase, recognizes either the AvrPto or AvrPtoB effector from Pseudomonas syringae pv tomato. This...Full Text Available

2010-01-01

116

Synthesis and stereochemical effects of pyrrolidinyl-acetylenic thieno[3,2-d]pyrimidines as EGFR and ErbB-2 inhibitors

The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in...Full Text Available

2004-07-01

117

British Library Electronic Table of Contents (United Kingdom)

A novel class of pyrrolidinyl-acetyleneic thieno[3,2-d]pyrimidines has been identified which potently inhibit the EGFR and ErbB-2 receptor tyrosine kinases. Synthetic modifications of the pyrrolidine carbamate moiety result in a range of effects on enzyme and cellular potency. In addition, the impact of the absolute stereochemical configuration on cellular potency and oral mouse pharmacokinetics is described.

2009-01-01

118

Synergistic Activation of Glucose-6-Phosphate Dehydrogenase and NAD(P)H oxidase by Src kinase Elevates Superoxide in Type 2 Diabetic, Zucker fa/fa, Rat Liver

Selective Expression in Carotid Body Type I Cells of a Single Splice Variant of the Large Conductance Calcium- and Voltage-activated Potassium Channel Confers Regulation by AMP-activated Protein Kinase*

Glucose metabolism through glycolysis and hexosamine pathway has been shown to be altered in type 2 diabetes. However, its fate through the pentose phosphate pathway (PPP) is currently unclear....Full Text Available

2009-08-01

119

Scyl1 Facilitates Nuclear tRNA Export in Mammalian Cells by Acting at the Nuclear Pore Complex

Inhibition of large conductance calcium-activated potassium (BK_Ca) channels mediates, in part, oxygen sensing by carotid body type I cells. However, BK_Ca channels remain active...Full Text Available

2011-04-08

120

ReSETting PP2A tumour suppressor activity in blast crisis and imatinib-resistant chronic myelogenous leukaemia

Scyl1 is an evolutionarily conserved N-terminal protein kinase-like domain protein that plays a role in COP1-mediated retrograde protein trafficking in mammalian cells. Furthermore, loss of Scyl1 function...Full Text Available

2010-07-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

121

Rapid inhibition of vasoconstriction in renal afferent arterioles by aldosterone

The deregulated kinase activity of p210-BCR/ABL oncoproteins, hallmark of chronic myelogenous leukaemia (CML), induces and sustains the leukaemic phenotype, and contributes to disease progression....Full Text Available

2006-10-09

122

Primitive hematopoietic cells resist HIV-1 infection via p21^{Waf1/Cip1/Sdi1}

Aldosterone has been suggested to elicit vessel contraction via a nongenomic mechanism. We tested this proposal in microdissected, perfused rabbit renal afferent arterioles. Aldosterone had no effect on internal diameter in concentrations from 10(-10) to 10(-5) mol/L, but aldosterone abolished the ability of 100 mmol/L KCl to induce vascular contraction. The inhibitory effect of aldosterone was observed from 1 pmol/L. The inhibitory effect was significant after 5 minutes and maximal after 20 minutes and was fully reversible. Actinomycin D (10(-6) mol/L) prolonged the effect of aldosterone. The effect was abolished by the mineralocorticoid receptor antagonist spironolactone (10(-7) mol/L) but not by the glucocorticoid receptor antagonist mifepristone (10(-6) mol/L). The K+-mediated increase of intracellular calcium concentration in afferent arterioles was not affected by aldosterone. Mineralocorticoid receptor was detected by reverse transcription-polymerase chain reaction and ...

2003-01-01

123

Overexpression of Partner of Numb Induces Asymmetric Distribution of the PI4P 5-Kinase Skittles in Mitotic Sensory Organ Precursor Cells in Drosophila

Hematopoietic stem cells are resistant to HIV-1 infection. Here, we report a novel mechanism by which the cyclin-dependent kinase inhibitor (CKI) p21^{Waf1/Cip1/Sdi1} (p21), a known regulator...Full Text Available

2007-02-01

124

Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase.

Unequal segregation of cell fate determinants at mitosis is a conserved mechanism whereby cell fate diversity can be generated during development. In Drosophila, each sensory organ...Full Text Available

125

NPM-ALK and the JunB transcription factor regulate the expression of cytotoxic molecules in ALK-positive, anaplastic large cell lymphoma

A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named...Full Text Available

1991-10-01

126

Mutational Analysis of the Carboxy-Terminal (YGX)₄ Repeat Domain of CpsD, an Autophosphorylating Tyrosine Kinase Required for Capsule Biosynthesis in Streptococcus pneumoniae

Anaplastic lymphoma kinase-positive, anaplastic large cell lymphoma (ALK+ ALCL) is an aggressive non-Hodgkin lymphoma of T/null immunophenotype that is most prevalent in children and young adults. The...Full Text Available

2011-02-15

127

Multistage skin tumor promotion: involvement of a protein kinase

In Streptococcus pneumoniae, CpsB, CpsC, and CpsD are essential for encapsulation, and mutants containing deletions of cpsB, cpsC, or cpsD...Full Text Available

2003-05-01

128

Mobilization of the genetically engineered plasmid pHSV106 from Escherichia coli HB101(pHSV106) to Enterobacter cloacae in drinking water.

Current information suggests that chemical carcinogenesis is a multistep process with one of the best studied models in this regard being the two-stage carcinogenesis system using mouse skin. The effects of several carcinogens and tumor promoters in various sequences of application were studied to examine the nature of the process. The actions of several tumor inhibitors were compared. (ACR)

1980-01-01

129

Mechano-transduction in Osteoblastic Cells Involves Strain-regulated Estrogen Receptor ?-mediated Control of Insulin-like Growth Factor (IGF) I Receptor Sensitivity to Ambient IGF, Leading to Phosphatidylinositol 3-Kinase/AKT-dependent Wnt/LRP5 Receptor-independent Activation of ?-Catenin Signaling*

We have used triparental matings to demonstrate transfer (mobilization) of the nonconjugative genetically engineered plasmid pHSV106, which contains the thymidine kinase gene of herpes simplex virus...Full Text Available

1991-01-01

130

Magnesium Sensitizes Slow Vacuolar Channels to Physiological Cytosolic Calcium and Inhibits Fast Vacuolar Channels in Fava Bean Guard Cell Vacuoles1

The capacity of bones to adjust their mass and architecture to withstand the loads of everyday activity derives from the ability of their resident cells to respond appropriately to the strains engendered....Full Text Available

2010-03-19

131

Isoform-Specific Functions of Phosphoinositide 3-Kinases: p110? but Not p110? Promotes Optimal Allergic Responses In Vivo1

Vacuolar ion channels in guard cells play important roles during stomatal movement and are regulated by many factors including Ca²⁺, calmodulin, protein kinases, and phosphatases....Full Text Available

1999-11-01

132

Involvement of protein kinase C in the mechanism of action of Escherichia coli heat-stable enterotoxin (STa) in a human colonic carcinoma cell line, COLO-205

The leukocyte-enriched p110γ and p110δ isoforms of PI3K have been shown to control in vitro degranulation of mast cells induced by cross-linking...Full Text Available

2008-02-15

133

Insulin-like growth factor II receptor is phosphorylated by a tyrosine kinase in adipocyte plasma membranes

The present study was undertaken to determine the involvement of calcium-protein kinase C pathway in the mechanism of action of Escherichia coli heat stable enterotoxin (STa) apart from STa-induced activation of guanylate cyclase in human colonic carcinoma cell line COLO-205, which was used as a model cultured cell line to study the mechanism of action of E. coli STa. In response to E. coli STa, protein kinase C (PKC) activity was increased in a time-dependent manner with its physical translocation from cytosol to membrane. Inhibition of the PKC activity in membrane fraction and inhibition of its physical translocation in response to IP_3-mediated calcium release inhibitor dantrolene suggested the involvement of intracellular store depletion in the regulation of PKC activity. Among different PKC isoforms, predominant involvement of calcium-dependent protein kinase C (PKC#alpha#) was specified using isotype-specific ...

2005-08-01

134

Forkhead Box Transcription Factor FOXO3a Regulates Estrogen Receptor Alpha Expression and Is Repressed by the Her-2/neu/Phosphatidylinositol 3-Kinase/Akt Signaling Pathway

Incorporation of /sup 32/P from (gamma-32P)ATP into tyrosine residues of the insulin-like growth factor (IGF)-II receptor was observed in a Triton X-100-insoluble fraction of rat adipocyte plasma membranes. IGF-II receptor phosphorylation proceeded to a stoichiometry of approximately 0.5 mol of phosphate/IGF-II binding site after 10 min of incubation at 4 degrees C. A Km for ATP of 6 microM was calculated for this phosphorylation reaction. Addition of IGF-II caused an approximately 2-fold increase in tyrosine phosphorylation of the IGF-II receptor in this preparation. In contrast, phosphorylation of angiotensin II by the Triton X-100 washed membranes was not stimulated by IGF-II. Incubation of purified receptor immobilized on IGF-II agarose or of receptor-enriched low density microsomal membranes with (gamma-32P)ATP did not result in appreciable incorporation of (/sup 32/P)phosphate into the IGF-II receptor nor into exogenous substrates. These data suggest that the IGF-II receptor is ...

1986-06-15

135

Cordycepin Blocks Lung Injury-Associated Inflammation and Promotes BRCA1-Deficient Breast Cancer Cell Killing by Effectively Inhibiting PARP

The expression status of the estrogen receptor alpha (ERα) and that of the epidermal growth factor receptor Her-2/neu frequently correlate inversely in breast cancers. While ERα-dependent...Full Text Available

2004-10-01

136

Beta-estradiol attenuates hypoxic pulmonary hypertension by stabilizing the expression of p27^kip1in rats

Cordycepin has been shown to interfere with a myriad of molecular processes from RNA elongation to kinase activity, and prevents numerous inflammatory processes in animal models. Here we show in a mouse...Full Text Available

2011-09-01

137

Alterations in Adenylate Kinase Activity in Human PBMCs after In Vitro Exposure to Electromagnetic Field: Comparison between Extremely Low Frequency Electromagnetic Field (ELF) and Therapeutic Application of a Musically Modulated Electromagnetic Field (TAMMEF)

BackgroundPulmonary vascular structure remodeling (PVSR) is a hallmark of pulmonary hypertension. P27^kip1, one of critical cyclin-dependent kinase inhibitors, has been...Full Text Available

2010-01-01

138

A thermal hydraulic investigation on ADSR liquid lead target

This study investigated the effects of electromagnetic fields on enzymes involved in purine metabolism in human peripheral blood mononuclear cells in vitro. Cells were obtained from 20 volunteers. We...Full Text Available

2009-01-01

139

The response of small scale rigid targets to shallow buried explosive detonations

Computational fluid dynamics(CFD) code FLUENT was used to simulate the thermal hydraulic processes occurring in conceptual design of the accelerator-driven subcritical reactor(ADSR) liquid lead target. The purpose of the analysis is to investigate the thermal hydraulic characteristics of liquid lead as ADSR target material with various target geometries and injection locations of proton beam. In the calculation analysis, the local temperature of the liquid lead target rises to the boiling temperature very rapidly. When the proton beam is injected from the bottom of the target system, the duration time to reach the boiling temperature is longer and the temperature distribution is flatter than other cases.

1998-05-01

140

British Library Electronic Table of Contents (United Kingdom)

Experimental and computational investigations were performed in order to better understand the mechanical response of rigid targets with various geometries to the detonation of shallow buried explosives. The motion of the targets was measured by use of high-speed digital video photography. This work involved flat targets, targets that were downwardly convex, and targets that were downwardly concave with explosive charges located at various positions beneath the targets. It was observed that, in general, angled hulls - whether downwardly concave or convex - tended to reduce the amount of momentum imparted to the center of mass of the targets. Computations were performed by use of an arbitrary Langrangian-Eulerian treatment in a nonlinear finite element code. A model based on quasi-static te...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

Targeting a Ruthenium Complex to the Nucleus with Short Peptides

Target stations and beam dumps for the CERN SPS

In an effort to develop octahedral metal complexes as chemotherapeutic and diagnostic agents targeted to DNA, it is critical to optimize the properties of their cellular uptake. Appending d-octaarginine...Full Text Available

2010-05-15

142

Suitability of a New Plant Invader as a Target for Biological Control in Florida

The design of the absorber blocks for internal and external dumping of the SPS proton beam is discussed. In addition, the external target stations for slow and fast extracted proton beams are described.

1977-03-16

143

Nato SCI-12 on Camouflage Evaluation Workshop on Search and Target ...

... L. Sutton. 2000. Is the aquatic weed hygrophila, Hygrophila polysperma (Polemoniales: Acanthaceae), a suitable target for classical biological ... ...

144

FOR THIN AND THICK TARGETS - NASA Technical Report Server (NTRS)

Nato SCI-12 on Camouflage Evaluation Workshop on Search and Target Acquisition The Netherlands, June 1999. Image Discrimination Models for Object Detection ...

145

Target area chamber system design for the National Ignition Facility

By W. Wayne Scott. Langley Research Center. SUMMARY. Thin- and thick-target bremsstrahlung spectra are presented for electron energies up to 7.0 MeV. ...

146

The National Ignition Facility (NIF) is a proposed Department of Energy facility which will contribute to the resolution of important Defense Program and inertial fusion energy issues for energy production in the future. The NIF will consist of a laser system with 192 independent beamlets transported to a target chamber. The target chamber is a multi-purpose structure that provides the interface between the target and the laser optics. The chamber must be capable of achieving moderate vacuum levels in reasonable times; it must remain dimensionally stable within micron tolerances, provide support for the optics, diagnostics, and target positioner; it must minimize the debris from the x-ray and laser light environments; and it must be capable of supporting external neutron shielding. The chamber must also be fabricated from a low neutron activation material. The fusion reaction in the ...

1994-06-19

147

Target R and D at JAERI

Optical System Design for Thermal Target Recognition by Spiral Scanning [TRSS

We proposed a solid and a mercury target concepts through the preliminary conceptual design. To feasible these concepts, analysis and experimental works are being carried out. This paper introduces an outline of present status of target R and D such as heat transfer augmentation experiments for the solid target, mercury flow tests with a loop of maximum flow rate of 15L/min, flow pattern measurements for a cold source moderator etc. as well as preliminary conceptual design works. (author)

1999-03-01

148

Inelastic scattering and neutron transfer induced by {sup 11}Be on a heavy target nucleus

Full Text Available

2004-12-01

149

Cryogenic heat pipe-target with aluminum condenser for the COSY-TOF experiment

Short communication.

1994-12-31

150

A Design of Position Tracking System for Moving Targets with Multi-Sensors

No abstract prepared.

2002-03-01

151

A He-gas Cooled, Stationary Granular Target

Full Text Available

2010-01-01

152

Polyphenol rich botanicals used as food supplements interfere with EphA2-ephrinA1 system

In the CERN approach to the design of a neutrino factory, the repetition frequency of the proton beam is high enough to consider stationary solid targets as a viable solution for multi-MW beams. The target consists of high density tantalum spheres of 2 mm diameter which can efficiently be cooled by passing a high mass flow He-gas stream through the voids between the Ta-granules. Very small thermal shocks and stresses will arise in this fine grained structure due to the relatively long burst of 3.3 ms from the SPL-proton linac. In a quadruple target system where each target receives only one quarter of the total beam power of 4 MW, conservative temperature levels and adequate lifetimes of the target are estimated in its very high radiation environment. A conceptual design of the integration of the target into the magnetic horn-pion-collector is presented.

2003-01-01

153

British Library Electronic Table of Contents (United Kingdom)

The Eph tyrosine kinase receptors and their ephrin ligands play a central role in several human cancers and their deregulated expression or function promotes tumorigenesis, inducing aggressive tumor phenotypes. Green tea extracts (GTE) have been recently found to inhibit Eph-kinase phosphorylation. In order to evaluate the potential contribution of edible and medicinal plants on EphA2-ephrinA1 modulation, 133 commercially available plant extracts used as food supplements, essential and fixed oils were screened with an ELISA-based binding assay. Nine plant extracts, rich of polyphenols, reversibly inhibited binding in a dose-dependent manner (IC"5"0 0.83-24@mg/ml). Functional studies on PC3 prostate adenocarcinoma cells revealed that active extracts antagonized ephrinA1-Fc-induced EphA2-pho...

2011-01-01

154

Phosphorylation of proteins in Clostridium thermohydrosulfuricum

Mechanism of adenylate kinase. Dose adenosine 5'-triphosphate bind to the adenosine 5'-monophosphate site

Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd polyamines characteristic of thermophiles, modulated the labeling of most of the ...

1986-02-01

155

In vivo phosphorylation of histones H1 and H5 in calf thymus and chicken erythrocytes as studied by /sup 31/P nuclear magnetic resonance spectroscopy

Although the subtrate binding properties of adenylate kinase (AK) have been studied extensively by various biochemical and biophysical techniques, it remains controversial whether uncomplexed adenosine 5'-triphosphate (ATP) binds to the adenosine 5'-monophosphate (AMP) site of AK. The authors present two sets of experiments which argue against binding of ATP to the AMP site. (a) /sup 31/P nuclear magnetic resonance titration of ATP with AK indicated a 1:1 stoichiometry on the basis of changes in coupling constants and line widths. This ruled out binding of ATP to both sites. (b) ATP and MgATP were found to behave similarly by protecting AK from spontaneous inactivation while AMP showed only a small degree of protection. Such inactivation could also be protected or reversed by dithioerythritol and is most likely due to oxidation of sulfhydryl groups, one of which (cysteine-25) is located near the MgATP site. The results support binding of ATP to ...

1987-10-06

156

Gene discovery in the Acanthamoeba castellanii genome

The /sup 31/P NMR method was first applied to characterize in vivo phosphorylation of H1 and H5 in calf thymus and chicken erythrocytes as well as in vitro phosphorylation of H1 and H5 by cAMP-dependent protein kinase. The amino acid residues phosphorylated in vivo in the histones were exclusively serine residues, and the mole fraction of phosphoserine was estimated to be 0.34 and 0.27 per molecule of calf thymus H1 and chicken erythrocyte H5, respectively. Interestingly, chicken erythrocyte H1 was not phosphorylated in vivo. Three H1 subtypes from calf thymus H1 varied in the /sup 31/P NMR spectra, and the bisected fragments of calf thymus H1 and chicken erythrocyte H5 exhibited characteristic spectral patterns, indicating that there are considerable diversities of the degree of phosphorylation and phosphorylation sites in very-lysine-rich histones. Furthermore, it was found that the microenvironment of phosphoserine residues phosphorylated in vivo in calf thymus ...

1987-08-01

157

GROWTH REGULATION IN ROUS SARCOMA VIRUS INFECTED CHICKEN EMBRYO FIBROBLASTS: THE ROLE OF THE src GENE

Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria. Acanthamoeba castellanii is also an opportunistic pathogen of clinical interest, responsible for several distinct diseases in humans. In order to provide a genomic platform for the study of this ubiquitous and important protist, we generated a sequence survey of approximately 0.5 x coverage of the genome. The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A. castellanii to inhabit adversity of environments. Alginate lyase may provide access to bacteria within biofilms by breaking down the biofilm matrix, and polyhydroxybutyrate depolymerase may facilitate utilization of the bacterial storage compound polyhydroxybutyrate as a food source. Enzymes for the synthesis and breakdown of cellulose were ...

2005-08-01

158

Cyclin-Dependent Kinase Inhibitor p16 and p21 Expression, and Cell Cycle Change in Human Lens Epithelial Cell Line SRA 01/04 following Contact Inhibition in Normal Culture

We report here a study of the mechanisms leading to loss of growth control in chicken embryo fibroblasts transformed by Rous sarcoma virus (RSV). We have been particularly concerned with the role of the src gene in this process, and have used RSV mutants temperature sensitive (ts) for transformation to investigate the nature of the growth regulatory lesion. The two principal findings were (1) the stationary phase of the cell cycle (G{sub 1}) in chick embryo fibroblasts seems to have two distinct regulatory compartments (using the terminology of Brooks et al. we refer to these as 'Q' and 'A' states). When rendered stationary at 41.5 C by serum deprivation, normal cells enter a Q state, but cells infected with the ts-mutant occupy an A state. (2) Whereas normal cells can occupy either state depending on culture conditions, the ts-infected cells, at 41.5 C, do not seem to enter Q even though a known src gene product, a kinase, is ...

1980-07-01

159

British Library Electronic Table of Contents (United Kingdom)

Abstract Purpose: To describe the pattern of expression of the cyclin-dependent kinase inhibitors (CDKIs) p16, p21 and p27, and the cell cycle in SRA 01/04 cells relative to contact inhibition. Methods: SRA 01/04 cells were grown to overconfluence under normal conditions. At various phases of the cell growth, cells were assayed by flow cytometry and Western blotting for the expression of CDKIs. Results: Expression of p16 was detected from early logarithmic growth to stationary phases, during which the number of cells in G0/G1 increased from 46 to 69%. Expression of p21 was detected only during the overgrowth phase, when 60% of the cells were in G0/G1. Expression of p27 was not observed in SRA 01/04 cells. Conclusions: p16 expression was likely mediated by G0/G1 arrest to induce contact inh...

2011-01-01

160

Changes in intracellular Ca2+ levels induced by cytokines and P2 agonists differentially modulate proliferation or commitment with macrophage differentiation in murine hematopoietic cells.

Calmodulin Kinase II Interacts with the Dopamine Transporter C Terminus to Regulate Amphetamine-Induced Reverse Transport

The role of intracellular Ca2+ (Ca2+i) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca2+i concentration ([Ca2+]i) with activation of phospholipase Cgamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. In addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1+ population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+]i, moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit+ population, and differentiation into macrophages without participation of GJ. It is likely that Ca2+i participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+]i would be ...

2008-09-05

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

161

Friend Spleen Focus-Forming Virus Activates the Tyrosine Kinase sf-Stk and the Transcription Factor PU.1 to Cause a Multi-Stage Erythroleukemia in Mice.

Efflux of dopamine through the dopamine transporter (DAT) is critical for the psychostimulatory properties of amphetamines, but the underlying mechanism is unclear. Here we show that Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) plays a key role in this efflux. CaMKIIalpha bound to the distal C terminus of DAT and colocalized with DAT in dopaminergic neurons. CaMKIIalpha stimulated dopamine efflux via DAT in response to amphetamine in heterologous cells and in dopaminergic neurons. CaMKIIalpha phosphorylated serines in the distal N terminus of DAT in vitro, and mutation of these serines eliminated the stimulatory effects of CaMKIIalpha. A mutation of the DAT C terminus impairing CaMKIIalpha binding also impaired amphetamine-induced dopamine efflux. An in vivo role for CaMKII was supported by chronoamperometry measurements showing reduced amphetamine-induced dopamine efflux in response to the CaMKII inhibitor KN93. Our data suggest that CaMKIIalpha binding ...

2006-01-01

162

Variation in the Definition of Clinical Target Volumes for Pelvic Nodal Conformal Radiation Therapy for Prostate Cancer

HEMATOLOGICAL MALIGNANCIES IN HUMANS TYPICALLY INVOLVE TWO TYPES OF GENETIC CHANGES: those that promote hematopoietic cell proliferation and survival (often the result of activation of tyrosine kinases) and those that impair hematopoietic cell differentiation (often the result of changes in transcription factors). The multi-stage erythroleukemia induced in mice by Friend spleen focus-forming virus (SFFV) is an excellent animal model for studying the molecular basis for both of these changes. Significant progress has been made in understanding the molecular basis for the multi-stage erythroleukemia induced by Friend SFFV. In the first stage of leukemia, the envelope protein encoded by SFFV interacts with and activates the erythropoietin (Epo) receptor and the receptor tyrosine kinase sf-Stk in erythroid cells, causing their Epo-independent proliferation, differentiation and survival. In the second stage, SFFV integration into the Sfpi1 locus ...

2010-10-11

163

Targeted nanoparticles that deliver a sustained, specific release of paclitaxel to irradiated tumors

PurposeWe conducted a comparative study of Clinical Target Volume (CTV) definition of pelvic lymph nodes by multiple GU radiation oncologists looking at the levels...Full Text Available

2009-06-01

164

Targeted Deletion of Somatotroph Insulin-Like Growth Factor-I Signaling in a Cell-Specific Knockout Mouse Model

To capitalize on the response of tumor cells to ionizing radiation, we developed a controlled-release nanoparticle drug delivery system using a targeting peptide that recognizes a radiation-induced...Full Text Available

2010-06-01

165

Specific genetic modifications of domestic animals by gene targeting and animal cloning

The role of IGF-I in the negative regulation of GH expression and release is demonstrated by in vitro and in vivo models; however, the targets and mechanisms of IGF-I...Full Text Available

2010-05-01

166

Selective imaging of adherent targeted ultrasound contrast agents

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available

167

National Newborn Screening Status Reports

The goal of ultrasonic molecular imaging is the detection of targeted contrast agents bound to receptors on endothelial cells. We propose imaging methods that can distinguish adherent microbubbles...Full Text Available

2007-04-21

168

Inhibitors of RecA Activity Discovered by High-Throughput Screening: Cell-Permeable Small Molecules Attenuate the SOS Response in Escherichia coli

... detected (and reported) as a by-product of MRM screening (MS/MS) targeted by Law or Rule ... detected (and reported) as a by-product of MRM screening (MS/MS) targeted by Law or Rule ...

169

How DNA coiling enhances target localization by proteins

The phenomenon of antibiotic resistance has created a need for the development of novel antibiotic classes with non-classical cellular targets. Unfortunately, target-based drug discovery against...Full Text Available

2009-10-01

170

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

2008-10-14

171

Efficient use of accessibility in microRNA target prediction

Peroxisomes, glyoxysomes, glycosomes, and hydrogenosomes have each been classified as microbodies, i.e., subcellular organelles with an electron-dense matrix that is bound by a single membrane. We investigated...Full Text Available

1991-09-01

172

Coordination of PAD4 and HDAC2 in the regulation of p53 target gene expression

Considering accessibility of the 3′UTR is believed to increase the precision of microRNA target predictions. We show that, contrary to common belief, ranking by the hybridization energy or by...Full Text Available

2011-01-01

173

Concurrent schedule control of human visual target fixations1

Histone Arg methylation and Lys acetylation have been found to cooperatively regulate the expression of p53 target genes. Peptidylarginine deiminase 4 (PAD4) is an enzyme that citrullinates...Full Text Available

2010-05-27

174

Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II

Operant conditioning techniques were applied to the study of how target fixations are controlled by the probability of signal occurrence. In a standard vigilance setting, gaze at three illuminable...Full Text Available

1973-11-01

175

Assessment of nodal target definition and dosimetry using three different techniques: implications for re-defining the optimal pelvic field in endometrial cancer

Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with...Full Text Available

2010-12-27

176

Three-quasiparticle states in "1"7"7Ta

Purposes1. To determine the optimal pelvic nodal clinical target volume for post-operative treatment of endometrial cancer. 2. To compare the DVH of different treatment planning...Full Text Available

177

Terrorism Knowledge Base (TKB)

... transitions lutetium 175 target mev range 10-100 multipolarity neutrons parity

178

Surgical Counter-Terrorism: Targeting Individuals as an ...

... they assisted in the 2000 attack on the USS Cole? ... List the types of targets that ETA has attacked. ... was at least partially responsible for the attack. ...

2008-04-01

179

Single-Walled Carbon Nanotubes Targeted to the Tumor ...

... Tanzania, and the attack on the USS COLE. ... vis another has no bearing on the legality of the attack. If the person attacked is a combatant, the use of ...

2003-02-03

180

Review of Vaccinia Virus and Baculovirus Viability Versus ...

... 1981. Retention of diphenyls, terphenyls, phenylalkanes and fluorene on graphitized thermal carbon black. Chromatographia 14:510-514. ...

2009-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

181

Production of polarized negative ion beams by collisional pumping

... hazards. One study targeted the effects of common household disinfectants on virus inactivation (Butcher and Ulaeto, 2005). ...

2008-03-01

182

Production of polarized negative ion beams by ''collisional pumping''

The production of polarized negative ion beams by collisional pumping is described. Collisional pumping utilizes repeated charge changing collisions in a thick electron-spin-polarized gas or vapor target to form a polarized fast atom beam. The polarized fast atom beam is then partially converted into a polarized negative ion beam in a vapor target. Analysis is presented for a hydrogen beam passing through either a thick polarized H atom target or a thick polarized alkali target. Large polarizations and large currents may be possible.

1983-01-01

183

Preparation of carrier-free yttrium-88 from strontium target by solvent extraction

The production of polarized negative ion beams by ''collisional pumping'' is described. Collisional pumping utilizes repeated charge changing collisions in a thick electron-spin-polarized gas or vapor target to form a polarized fast atom beam. The polarized fast atom beam is then partially converted into a polarized negative ion beam in a vapor target. Analysis is presented for a hydrogen beam passing through either a thick polarized H atom target or a thick polarized alkali target. Large polarizations and large currents may be possible.

1984-03-01

184

Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

... carrier-free isotopes chemical preparation gamma spectra isotope production

185

Pairing effect in the nucleon transfer processes in quasi-elastic heavy ion scattering

Full Text Available

2009-10-01

186

Market segmentation and targeting for real time pricing

... range krypton 86 reactions molybdenum 92 target probability quasi-elastic

1987-04-14

187

Fragmentation of nuclei by particles and nuclei of intermediate and high energies

While there is growing interest in Real Time Pricing as a competitive tool, its adoption to date has been constrained by wariness on the part of many utilities and many of their customers. That wariness is not misplaced, for Real Time Pricing is not for everyone. In order for both utilities and their customers to benefit, the pricing system must be properly tailored for, and offered to, the appropriate audience. This paper discusses needs for identifying targets, implications of targeting of Real Time Pricing to various types of customers, and implications for design and marketing of such pricing systems. Examples from utility experiences are provided, and recommendations are offered for development of improved targeting of Real Time Pricing.

1996-03-01

188

Experimental determination of the ion-ion potential in the N=50 target region: a tool to probe ground-state nuclear densities

The present state of investigations into the fragmentation of target nuclei by particles and nuclei of intermediate and high energies is reviewed.

1983-11-01

189

Electron-Induced Luminescence and X-Ray Spectrometer (ELXS) System for Life Detection

No abstract prepared.

1999-07-01

190

Electromagnetic excitations in nuclei: From photon scattering to photo-dissociation

The ELXS concept is a novel, portable, micro-instrument targeted for the detection of mineralogic

2002-01-01

191

Effect of improved target designs on the "2"3"8Pu production at the Fast Flux Test Reactor

... 98 target molybdenum 99 niobium 91 photoneutrons photonuclear reactions

2006-03-20

192

Target space duality II: applications

This paper present the results of a series of calculations made to determine the "2"3"8Pu production potential of several advanced target assembly designs in the Fast Flux Test Facility (FFTF). These calculations show that by using advanced target designs the intimately mix the "2"3"7Np target material with an yttrium hydride moderator, the FFTF has the potential of producing up to 30 kg of high-quality "2"3"8Pu per year.

1991-11-10

193

Gastrin releasing peptide (GRP) receptor targeted radiopharmaceuticals: A concise update

We apply the framework developed in Target space duality I: general theory. We show that both nonabelian duality and Poisson-Lie duality are examples of the general theory. We propose how the formalism leads to a systematic study of duality by studying few scenarios that lead to open questions in the theory of Lie algebras. We present evidence that there are probably new examples of irreducible target space duality.

2000-09-25

194

Effluent reduction using pinch technology: Targets for reduction and capital costs for mass exchange networks

The gastrin releasing peptide (GRP) receptor is becoming an increasingly attractive target for development of new radiolabeled peptides with diagnostic and therapeutic potential. The attractiveness of the GRP receptor as a target is based upon the functional expression of GRP receptors in several tumors of neuroendocrine origin including prostate, breast, and small cell lung cancer. This concise review outlines some of the efforts currently underway to develop new GRP receptor specific radiopharmaceuticals by employing a variety of radiometal chelation systems.

2003-11-01

195

Development of a new secondary beam separator and a new gas-jet target at Kyushu University

This paper illustrates how the techniques developed by the authors for capital cost targeting of mass exchange networks can be applied to determination of capital investment targets for reduction in effluent for existing systems involving mass exchange. The results is an impact diagram which shows the relationship between effluent reduction and capital investment, indicating a region of limiting return on investment as well as the maximum possible reduction in effluent. (au)

1999-02-01

196

Detection device for high explosives

In order to facilitate observations of low energy nuclear reactions, a new type recoil mass-separator together with a new gas-jet target system is being developed at the tandem accelerator facility in Kyushu University. The expected mass-resolving power of the separator is 220 for a solid angle of 10 msr and the practical thickness of the gas-jet target will exceed 0.1 atm#centre dot#cm for the light elements of H and He. (author).

1994-06-01

197

A Liquid Parahydrogen Target for the Measurement of a Parity-violating Gamma Asymmetry in Polarized Neutron Capture on Protons

A portable fiber optic detector that senses the presence of specific target chemicals by electrostatically attracting the target chemical to an aromatic compound coating on an optical fiber. Attaching the target chemical to the coated fiber reduces the fluorescence so that a photon sensing detector records the reduced light level and activates an appropriate alarm or indicator.

1992-01-01

198

48 CFR 952.226-73 - Energy Policy Act target group certification.

A 16 l liquid parahydrogen target has been developed for a measurement of the parity-violating {gamma}-asymmetry in the capture of polarized cold neutrons on protons in the {rvec n} + p {yields} d + {gamma} reaction by the NPDGamma collaboration. The target system was carefully designed to meet the stringent requirements on systematic effects for the experiment and also to satisfy hydrogen safety requirements. The target was designed to preserve the neutron polarization during neutron scattering on liquid hydrogen (LH{sub 2}), optimize the statistical sensitivity to the {rvec n} + p {yields} d + {gamma} reaction, minimize backgrounds coming from neutron interaction with the beam windows of the target cryostat, minimize LH{sub 2} density fluctuations which can introduce extra noise in the gamma asymmetry signal, and control systematic effects. The target incorporates two mechanical ...

2010-05-01

199

Selection of IFE target materials from a safety and environmental perspective

...2010-10-01 2010-10-01 false Energy Policy Act target group certification...Acquisition Regulations System DEPARTMENT OF ENERGY CLAUSES AND FORMS SOLICITATION...Provisions and Clauses 952.226-73 Energy Policy Act target group...

2010-10-01

200

Mode of action of membrane-disruptive lytic compounds from the marine dinoflagellate Alexandrium tamarense

Target materials for inertial fusion energy (IFE) power plant designs might be selected for a wide variety of reasons including wall absorption of driver energy, material opacity, cost and ease of fabrication. While each of these issues are of great importance, target materials should also be selected based upon their safety and environmental (S and E) characteristics. The present work focuses on the recycling, waste management and accident dose characteristics of potential target materials. If target materials are recycled so that the quantity is small, isotopic separation may be economically viable. Therefore, calculations have been completed for all stable isotopes for all elements from lithium to polonium. The results of these calculations are used to identify specific isotopes and elements that are most likely to be offensive as well as those most likely to be acceptable in terms of their S and E ...

2001-05-21

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

201

British Library Electronic Table of Contents (United Kingdom)

Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined. We first hypothesized that membrane sterols serve as molecular targets of these lytic compounds, and that differences in sterol composition among donor and target cells may cause insensitivity of Alexandrium and sensitivity of targets to lytic compounds. We investigated Ca^2^+ influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes. The lytic compounds increased permeability of the cell membrane for Ca^2^+ ions even during blockade of Ca^2^+ channels wit...

2011-01-01

202

An identification method of positron production in laser beam interaction with targets

Activation analysis of target debris in the national ignition facility

A simple electromagnetic transport system was constructed to identify very rare positrons produced in a powerful laser beam interaction with a target. Testing experiments were carried out with CO[sub 2]-laser (10[sup 12] W/cm[sup 2]) beam pulses ([tau] = 50 ns, f = 0.01 Hz) focused on the copper target, as well as with a 96 MeV alpha-particle beam irradiated carbon target. The results showed that the developed system could be effectively used for positron identification and evaluation of their energy by means of a time-of-flight method. The computerized system to deal with this problem, together with others related to the power laser beam interaction with targets, has been constructed. (orig.).

1992-10-01

203

[Improvement of the recognition probability about camouflage target based on BP neural network].

The modeling methods used to compute the neutron-induced activation of target and near-target materials in the NIF facility are presented. A detailed space and energy description of the neutron environment in the different materials is provided. A new capability has been developed to treat in a general way the activation of debris produced in an operational regime of yield and no-yield experiments. First calculations are addressed to analyze the activity of the debris into the target chamber. The contribution of the different components to activity, interior dose rates, and waste disposal rating (WDR) is determined. The importance of the activation coming from primary irradiation in the target, and from secondary irradiation in debris deposited onto the first wall is assessed. Finally, waste hazards of the activated debris when removed out of the chamber and stored are analyzed. (authors)

1999-09-12

204

Traffic and transport in the Dutch National Environmental Outlook 4; Verkeer en vervoer in de Nationale Milieuverkenning 4

Using static Michelson interferometer to get the spectrum information of measurement targets for spectrum identification, under the condition that the interference length is constant, the system can be optimized by BP neural network algorithm for the mixed spectral separation process. Thereby it can realize improving the recognition probability of camouflage target. Collecting the spectrum information in field of view (FOV) by the interferometer and linear array CCD detector, composing the set of mixed spectrum data, with known absorption spectrum of the material as a hidden layer of rules, it used BP neural network to separate the mixed spectrum data. Experiment with different distances, different combinations of mixed background spectrum as the initial data, using steel target (size: 1.5 m x 1.5 m) made of four kinds, the recognition probability of non-camouflage target is about 90% by BP neural ...

2010-12-01

205

Tungsten Effects on Phosphate-Dependent Biochemical Pathways are Species and Liver Cell Line Dependent

In the Fourth Dutch National Environmental Outlook (NMP, abbreviated in Dutch) of the National Institute of Public Health and the Environment (VROM), published in July 1997, possible future developments in the traffic and transport sector in the Netherlands are described for the period 1995-2020 and also evaluates present Dutch policies. NMP-4 also offers possible solutions for expected environmental problems. This report serves as a background document for the traffic and transport sector. With a view to present Dutch policies, the main conclusions drawn from the Outlook are that: (1) the policy targets for car and lorry use for 2010 will not be met, (2) the target for CO2 emissions from road transport for 2010 will not be met, (3) the NOx emission target for 2010 will be met for cars, but not for trucks, (4) the VOC emission target for 2010 will be met for cars, but not for trucks, and (5) the noise ...

1998-03-01

206

British Library Electronic Table of Contents (United Kingdom)

Tungsten, in the form of tungstate, polymerizes with phosphate, and as extensive polymerization occurs, cellular phosphorylation and dephosphorylation reactions may be disrupted, resulting in negative effects on cellular functions. A series of studies were conducted to evaluate the effect of tungsten on several phosphate-dependent intracellular functions, including energy cycling (ATP), regulation of enzyme activity (cytosolic protein tyrosine kinase [cytPTK] and tyrosine phosphatase), and intracellular secondary messengers (cyclic adenosine monophosphate [cAMP]). Rat noncancerous hepatocyte (Clone-9), rat cancerous hepatocyte (H4IIE), and human cancerous hepatocyte (HepG2) cells were exposed to 1-1000 mg/l tungsten (in the form of sodium tungstate) for 24 h, lysed, and analyzed for the ab...

2010-01-01

207

Tissue-specific expression of Fgfr2b and Fgfr2c isoforms, Fgf10 and Fgf9 in the developing chick mandible

British Library Electronic Table of Contents (United Kingdom)

SummaryExperimental evidence has demonstrated the importance of FGF signalling in morphogenesis of the mandibular processes. FGFs transmit their signals through four tyrosine kinase transmembrane receptors (FGFRs). Alternative splicing in FGFRs including FGFR2 generates different isoforms that exhibit different ligand-specificities, exclusive tissue distributions and specific biological functions. Despite extensive information regarding the isoform-specific patterns of expression Fgfr2c and Fgfr2b during morphogenesis of many organs, a comparative analysis of these specific isoforms in the chick mandible has not been reported. To better understand the function of FGFR2 in mandibular morphogenesis, we have analysed the expression Fgfr2b, Fgfr2c and their putative ligands Fgf10 and Fgf9, in ...

2006-01-01

208

Submitochondrial distribution of cAMP in its incubation with rat liver mitochondria

Small heat shock protein 20 (HspB6) in cardiac hypertrophy and failure

When labeled cAMP is incubated with isolated mitochondria from rat liver, it penetrates not only through the outer membrane of the mitochondria but also into the mitoplasts, where it is accumulated chiefly in the matrix. Damage to the mitochondrial membrane by freezing-thawing promotes not an influx but an efflux of cAMP out of the mitoplasts. A substantial part of the labeled nucleotide in all the investigated submitochondrial fractions was identified as intact cAMP by the method of thin-layer chromatography. The transport of cAMP into the mitochondria can explain the activation of their functions by the hormones that activate cytoplasmic adenylate cyclase and by extramitochondrial (cytosol) cAMP, as well as the presence of cAMP-dependent protein kinase and cAMP-phosphodiesterase in the mitochondria.

1986-03-20

209

British Library Electronic Table of Contents (United Kingdom)

Hsp20, referred to as HspB6, is constitutively expressed in various tissues. Specifically, HspB6 is most highly expressed in different types of muscle including vascular, airway, colonic, bladder, and uterine smooth muscle; cardiac muscle; and skeletal muscle. It can be phosphorylated at Ser-16 by both cAMP- and cGMP-dependent protein kinases (PKA/PKG). Recently, Hsp20 and its phosphorylation have been implicated in multiple physiological and pathophysiological processes including smooth muscle relaxation, platelet aggregation, exercise training, myocardial infarction, atherosclerosis, insulin resistance and Alzheimer's disease. In the heart, key advances have been made in elucidating the significance of Hsp20 in contractile function and cardioprotection over the last decade. This mini-rev...

2011-01-01

210

Metabolic and molecular stress responses of gilthead seam bream Sparus aurata during exposure to low ambient temperature: an analysis of mechanisms underlying the winter syndrome

British Library Electronic Table of Contents (United Kingdom)

The winter syndrome in the gilthead sea bream Sparus aurata indicates that the species is exposed to critically low temperatures in Mediterranean aquaculture in winter. The present study of metabolic patterns and molecular stress responses during cold exposure was carried out to investigate this ?disease?, in light of the recent concept of oxygen and capacity limited thermal tolerance. The metabolic profile of fuel oxidation was examined by determining the activities of the enzymes hexokinase (HK), aldolase (Ald), pyruvate kinase (PK), l-lactate dehydrogenase (l-LDH), citrate synthase (CS), malate dehydrogenase (MDH) and 3-hydroxyacyl CoA dehydrogenase (HOAD) in heart, red and white muscle after exposure to temperatures of 10, 14 and 18?C. Especially, the increase in LDH activity combined ...

2010-01-01

211

Mechanism of Dephosphorylation of the SR Protein ASF/SF2 by Protein Phosphatase 1

British Library Electronic Table of Contents (United Kingdom)

SR proteins are essential splicing factors whose function is controlled by multi-site phosphorylation of a C-terminal domain rich in arginine-serine repeats (RS domain). The protein kinase SRPK1 has been shown to polyphosphorylate the N-terminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear entry of this splicing factor and engagement in splicing function. Later, dephosphorylation is required for maturation of the spliceosome and other RNA processing steps. While phosphates are attached to RS1 in a sequential manner by SRPK1, little is known about how they are removed. To investigate factors that control dephosphorylation, we monitored region-specific mapping of phosphorylation sites in ASF/SF2 as a function of the protein phosphatase PP1. W...

2010-01-01

212

Decreased dendritic spine density and abnormal spine morphology in Fyn knockout mice

British Library Electronic Table of Contents (United Kingdom)

Fyn is a Src-family tyrosine kinase that affects long term potentiation (LTP), synapse formation, and learning and memory. Fyn is also implicated in dendritic spine formation both in vitro and in vivo. However, whether Fyn's regulation of dendritic spine formation is brain-region specific and age-dependent is unknown. In the present study, we systematically examined whether Fyn altered dendritic spine density and morphology in the cortex and hippocampus and if these effects were age-dependent. We found that Fyn knockout mice trended toward a decrease in dendritic spine density in cortical layers II/III, but not in the hippocampus, at 1month of age. Additionally, Fyn knockout mice had significantly decreased dendritic spine density in both the cortex and hippocampus at 3months and 1year, an...

2011-01-01

213

Cyclosporine metabolic side effects: association with the WNK4 system

British Library Electronic Table of Contents (United Kingdom)

Abstract Background- Cyclosporine is used for treatment of transplanted patients and for immune-mediated diseases. Cyclosporine is known to cause a combination of metabolic side effects including hypertension, hyperkalemia, hypercalciuria and hypomagnesemia. These side effects except for hypomagnesemia are the cardinal features of familial hyperkalemia and hypertension (FHHt), also called pseudohypoaldosteronism type II (PHA II). FHHt is caused by mutations in the kinases WNK1 and WNK4 resulting in an increase in renal Na-Cl cotransporter (NCC) apical distribution and function. Therefore, we studied whether cyclosporine-s metabolic side effects are mediated by WNK4 and NCC. Design- Sprague-Dawley (SD) rats were treated by cyclosporine 25-mg-kg-1 subcutaneously for 14-days. Blood pressure, ...

2011-01-01

214

BzpF is a CREB-like transcription factor that regulates spore maturation and stability in Dictyostelium

British Library Electronic Table of Contents (United Kingdom)

The cAMP response element-binding protein (CREB) is a highly conserved transcription factor that integrates signaling through the cAMP-dependent protein kinase A (PKA) in many eukaryotes. PKA plays a critical role in Dictyostelium development but no CREB homologue has been identified in this system. Here we show that Dictyostelium utilizes a CREB-like protein, BzpF, to integrate PKA signaling during late development. bzpF^- mutants produce compromised spores, which are extremely unstable and germination defective. Previously, we have found that BzpF binds the canonical CRE motif in vitro. In this paper, we determined the DNA binding specificity of BzpF using protein binding microarray (PBM) and showed that the motif with the highest specificity is a CRE-like sequence. BzpF is necessary to ...

2011-01-01

215

Accumulation, Activity and Localization of Cell Cycle Regulatory Proteins and the Chloroplast Division Protein FtsZ in the Alga Scenedesmus quadricauda under Inhibition of Nuclear DNA Replication

British Library Electronic Table of Contents (United Kingdom)

Synchronized cultures of the green alga Scenedesmus quadricauda were grown in the absence (untreated cultures) or in the presence (FdUrd-treated cultures) of 5-fluorodeoxyuridine, the specific inhibitor of nuclear DNA replication. The attainment of commitment points, at which the cells become committed to nuclear DNA replication, mitosis and cellular division, and the course of committed processes themselves were determined for cell cycle characterization. FdUrd-treated cultures showed nearly unaffected growth and attainment of the commitment points, while DNA replication(s), nuclear division(s) and protoplast fission(s) were blocked. Interestingly, the FdUrd-treated cells possessed a very high mitotic histone H1 kinase activity in the absence of any nuclear division(s). Compared with the ...

2008-01-01

216

Is uniform target dose possible in IMRT plans in the head and neck?

Radioisotope production in the I.Ph.P.E. cyclotron for medical application

Purpose: Various published reports involving intensity-modulated radiotherapy (IMRT) plans developed using automated optimization (inverse planning) have demonstrated highly conformal plans. These reported conformal IMRT plans involve significant target dose inhomogeneity, including both overdosage and underdosage within the target volume. In this study, we demonstrate the development of optimized beamlet IMRT plans that satisfy rigorous dose homogeneity requirements for all target volumes (e.g., #+-#5%), while also sparing the parotids and other normal structures. Methods and Materials: The treatment plans of 15 patients with oropharyngeal cancer who were previously treated with forward-planned multisegmental IMRT were planned again using an automated optimization system developed in-house. The optimization system allows for variable sized beamlets computed using a three-dimensional convolution/superposition dose ...

2002-04-01

217

Radioisotope production in the I. Ph. P. E. cyclotron for medical application

The production methods for seven radioisotopes, Ga-67, Sr-85, Pd-103, In-111, Tu-167, Hg-197 and Pb-203, by using a classical 1.5m cyclotron in the Institute of Physics and Power Engineering, Obninsk, USSR, are described. At present, more than 50 cyclotrons in different countries are used for the production of radioisotopes applied to medicine. Radioisotopes are produced with the cyclotron in the I.Ph.P.E. in the form of irradiated targets, which are delivered to Moscow radiopharmaceutical factory, where radiopharmaceuticals are produced on the base of these targets. The cyclotron is operated in two regimes providing the acceleration of protons, deuterons and alpha -particles. Two types of target assemblies are used for irradiation, the one is intended for the internal beam, and the other is for the external beam. The reactions used for the production of seven radioisotopes described above, the types of ...

218

Distortion-invariant color pattern recognition using multiple phase-shifted-reference-based joint transform correlation incorporating synthetic discriminant function

The production methods for seven radioisotopes, Ga-67, Sr-85, Pd-103, In-111, Tm-167, Hg-197 and Pb-203, by using a classical 1.5m cyclotron in the Institute of Physics and Power Engineering, Obninsk, USSR, are described. At present, more than 50 cyclotrons in different countries are used for the production of radioisotopes applied to medicine. Radioisotopes are produced with the cyclotron in the I.Ph.P.E. in the form of irradiated targets, which are delivered to Moscow radiopharmaceutical factory, where radiopharmaceuticals are produced on the base of these targets. The cyclotron is operated in two regimes providing the acceleration of protons, deuterons and alpha -particles. Two types of target assemblies are used for irradiation, the one is intended for the internal beam, and the other is for the external beam. The reactions used for the production of seven radioisotopes described above, the types of ...

1982-01-01

219

Mechanism of enzyme-catalyzed phospho group transfer

This paper proposes a new pattern recognition system employing optical joint transform correlation (JTC) technique which offers a great number of advantages over similar digital techniques, including very fast operation, simple architecture and capability of updating the reference image in real time. The proposed JTC technique incorporates a synthetic discriminant function (SDF) of the target image estimated from different training images to make the pattern recognition performance invariant to noise and distortion. It then involves four different phase-shifted versions of the same target SDF reference image, which are individually joint transform correlated with the given input scene. When the correlation signals are combined, it produces a single cross-correlation peak corresponding to each potential target present in the given input scene. The proposed technique also includes a fringe-adjusted filter to generate a ...

2011-04-01

220

Cysteinyl peptides of rabbit muscle pyruvate kinase labeled by the affinity label 8-((4-bromo-2,3-dioxobutyl)thio)adenosine 5 prime -triphosphate

To understand more fully the mechanism of enzyme-catalyzed phospho group transfer, the stereochemical course at phosphorus of four enzymes has been determined. First, using adenosine (..gamma..-(S)-/sup 16/O, /sup 17/O, /sup 18/O)triphosphate as the substrate, the reaction catalyzed by creatine kinase has been found to proceed with overall inversion of configuration at phosphorus. Second, using adenosine (..beta..-(S)-/sup 16/O, /sup 17/O, /sup 18/O)diphosphate as the substrate, the reaction catalyzed by adenylate kinase has been found also to proceed with overall inversion. Third, the reaction catalyzed by phosphoenolpyruvate carboxylase has been studied using ((S/sub p/)-/sup 16/O, /sup 17/O)thiophospoenolpyruvate as the substrate in H/sub 2/ /sup 18/O. Fourth, using adenosine 5'-O-((..gamma..S/sub p/)-..beta gamma..-/sup 17/O,..gamma..-/sup 17/O,/sup 18/O)(3-thiotriphosphate) as the substrate, the reaction catalyzed by pyruvate ...

1986-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

Characterization of the major phosphoprotein and its kinase on the surface of the rat adipocyte

The affinity label 8-((4-bromo-2,3-dioxobutyl)thio)adenosine 5{prime}-triphosphate (8-BDB-TA-5{prime}-TP) reacts covalently with rabbit muscle pyruvate kinase, incorporating 2 mol of reagent/mol of enzyme subunit upon complete inactivation. Protection against inactivation is provided by phosphoenolpyruvate, K{sup +}, and Mn{sup 2+} and only 1 mol of reagent/mol of subunit is incorporated. The authors have now identified the resultant modified residues. After reaction with 8-BDB-TA-5{prime}-TP at pH 7.0, modified enzyme was incubated with ({sup 3}H)NaBH{sub 4} to reduce the carbonyl groups of enzyme-bound 8-BDB-TA-5{prime}-TP and to introduce a radioactive tracer into the modified residues. Following carboxymethylation and digestion with trypsin, the radioactive peptides were separated on a phenylboronate agarose column followed by reverse-phase high-performance liquid chromatography in 0.1% trifluoroacetic acid with an acetonitrile gradient. Gas-phase sequencing ...

1990-03-13

222

Uses of laser optical pumping to produce polarized ion beams

Intact rat fat cell exposed to 12.5 ..mu..M (..gamma..-32P)ATP incorporate label into specific proteins within minutes. By solubilizing the reaction mixture with SDS which bypasses the subcellular fractionation steps, the labeled proteins can be identified in autoradiographs of SDS-PAGE gels. The most prominently labeled protein has an M/sub r/ of 42,000. Localization of this component to the cell surface can be made on the basis of inhibition of phosphorylation by addition of a protein derived from the rat brain with protein kinase inhibitory property, susceptibility of the phosphorylated protein to the tryptic digestion, inhibition of phosphorylation of this protein after brief exposure to melittin. To rule out the possibility that the cell surface protein might be a mitochondrial contaminant from broken cells, /sup 32/Pi-labeled and (..gamma..-/sup 32/P)ATP-labeled cells were chromatographed on a rabbit anti-pyruvate dehydrogenase antibody-Sepharose 4B column. A ...

1986-12-01

223

The HARP experiment first physics results

Laser optical pumping can be used to produce polarized alkali atom beams or polarized alkali vapor targets. Polarized alkali atom beams can be converted into polarized alkali ion beams, and polarized alkali vapor targets can be used to produce polarized H/sup -/ or /sup 3/He/sup -/ ion beams. In this paper the authors discuss how the polarized alkali atom beams and polarized alkali vapor targets are used to produce polarized ion beams with emphasis on the production of polarized negative ion beams.

1983-04-01

224

Separation of carrier-free "1"7"6","1"7"7W and "1"7"6","1"7"7Ta produced in "1"6O irradiated holmium target

The HARP experiment at CERN is performing extensive measurements of hadron production cross sections and secondary particle yields, in the momentum range 1.5-15 GeV/c, over the full solid angle and using a large set of cryogenic and solid targets. First measurements of hadron production cross-sections in the forward region are reported using an aluminium target 5% of an interaction length thick and a proton beam of 12.9 GeV/c. A preliminary analysis in the large angle region of elastic scattering events produced with the cryogenic hydrogen target at 3 GeV/c beam momentum is also presented.

2005-01-01

225

Separation of carrier free "1"7"6","1"7"7W and "1"7"6","1"7"7Ta radionuclides produced in "1"6O activated holmium metal target

Carrier-free radionuclides of tungsten and tantalum, "1"7"6","1"7"7W and "1"7"6","1"7"7Ta have been produced by heavy ion activation of holmium target with 97 MeV "1"6O"5"+ beam. Radiochemical separation scheme has been developed to isolate tungsten and tantalum radionuclides from the holmium target matrix. (author)

2001-11-01

226

Recovery of radioactive thallium isotopes from lead and bismuth targets irradiated by 1-GeV protons

Carrier-free radioisotopes of tungsten, "1"7"6","1"7"7W, and their corresponding daughter radionuclides "1"7"6","1"7"7Ta, have been produced in holmium target by heavy ion activation with "1"6O"5"+ beam. An attempt has been made to separate these carrier-free radionuclides from bulk holmium target through LLX using cation exchanger HDEHP. (author)

2001-02-07

227

Recovery of radioactive thallium isotopes from lead and bismuth targets irradiated by 1-GeV protons

A simple highly efficient procedure has been developed for recovery of thallium radioisotopes from lead and bismuth targets irradiated by 1-GeV protons. The procedure is based on the use of extraction chromatography. The cross-sections have been determined for formation of {sup 200}Pb, {sup 201}Pb, {sup 202m}Pb, and {sup 203}Pb radioisotopes in targets from lead with natural isotopic composition, irradiated by 1-GeV protons.

1995-03-01

228

Preliminary Study of Plasma Stream Interaction with Tungsten Target within RPI-IBIS Facility

A simple highly efficient procedure has been developed for recovery of thallium radioisotopes from lead and bismuth targets irradiated by 1-GeV protons. The procedure is based on the use of extraction chromatography. The cross-sections have been determined for formation of "2"0"0Pb, "2"0"1Pb, "2"0"2"mPb, and "2"0"3Pb radioisotopes in targets from lead with natural isotopic composition, irradiated by 1-GeV protons.

229

Modified-VSIMM algorithm with an application to the naval fire control technology

The paper presents results of experimental research on the interaction of a pulsed plasma-ion stream with a tungsten (W) target. The pulsed hydrogen plasma was produced within the RPI-IBIS (Multi-Rod Plasma Injector) facility at IPJ in Swierk. Measurements were carried out by means of optical spectroscopy and corpuscular diagnostic techniques. For experiments with the W-target the operational conditions (so-called PID mode) were chosen when a clean hydrogen plasma stream was generated. Attention was paid to the identification of WI and WII spectral lines.

2006-01-01

230

British Library Electronic Table of Contents (United Kingdom)

A modified variable structure interacting multiple model (M-VSIMM) estimator for complex hybrid maneuver target tracking is presented. The M-VSIMM could potentially be applied to fire control systems (FCS) used on warships. Target model groups were designed using 3D dimensional dynamic target models. Optimal model group selection logic was proposed, contrary to the activation and termination logic in the original VSIMM. The system will respond faster with optimal model group selection logic. After performing simulations, the tracking performances of the Kalman, ?-?(-?), VDIE, IMM and M-VSIMM filters were compared under various maneuvering conditions.

2011-01-01

231

Experiments to investigate the effects of radiative cooling on plasma jet collimation

Convoy electron production in polycrystalline and monocrystalline targets

Preliminary experiments have been performed to investigate the effects of radiative cooling on plasma jets. Thin (3 um - 5 um) conical shells were irradiated with an intense laser, driving jets with velocities > 100 km/s. Through use of different target materials - aluminium, copper and gold - the degree of radiative losses was altered, and their importance for jet collimation investigated. A number of temporally resoved optical diagnostics was used, providing information about the jet evolution. Gold jets were seen to be narrower than those from copper targets, while aluminium targets produced the least collimated flows.

2010-01-01

232

Computer-assisted rotation and multiple stationary irradiation technique

The velocity distribution of electrons ejected close to the forward direction by 0.8-2 MeV/A ions traversing various solid targets, including a Au monocrystal, is measured in coincidence with emerging charge-selected ions. The velocity spectrum is observed to be independent of outgoing projectile velocity and charge state for polycrystalline targets. Measurements on the Au crystal under channeling conditions show dependences on final charge state, and are tentatively explained by assuming that the main contribution to the production yield comes from the non-channeled fraction of the ions. A simple model for the creation of the forward-ejected electrons is proposed, which accounts for most of the experimental findings.

1980-01-01

233

Centroid and Envelope Dynamics of High-intensity Charged Particle Beams in an External Focusing Lattice and Oscillating Wobbler

A computer-assisted rotation and stationary conformation radiotherapy system with overrunning multileaf collimators has been developed. This system can produce any shape of target volume, including a number of target areas outside the axis of the rotation, regardless of the location of the axis at 360"0 rotation irradiation. In addition, by changing the dose rate and deleting a partial region within a field, multiple stationary irradiation can produce more homogeneous target volumes - thus avoiding excessive irradiation to critical organs - than the rotation technique. (orig.).

234

Yields of Residual Nuclei from Proton-Irradiated Materials

The centroid and envelope dynamics of a high-intensity charged particle beam are investigated as a beam smoothing technique to achieve uniform illumination over a suitably chosen region of the target for applications to ion-beam-driven high energy density physics and heavy ion fusion. The motion of the beam centroid projected onto the target follows a smooth pattern to achieve the desired illumination, for improved stability properties during the beam-target interaction. The centroid dynamics is controlled by an oscillating "wobbler", a set of electrically-biased plates driven by RF voltage. __________________________________________________

2010-04-28

235

International Science & Technology Center (ISTC)

Experimental and Theoretical Study of the Residual Nuclide Production in 40-2600 MeV Proton-Irradiated Thin Targets of ADS Basic Materials

236

Tuberculous Granulomas Are Hypoxic in Guinea Pigs, Rabbits, and Nonhuman Primates?

The skeletal muscle ryanodine receptor identified as a molecular target of[3H] azidodantrolene by photoaffinity labeling

Understanding the physical characteristics of the local microenvironment in which Mycobacterium tuberculosis resides is an important goal that may allow the targeting of metabolic processes...Full Text Available

2008-06-01

237

The influence of target backing on ion-beam electron spectra

... 16, 2001 0006-2979 BIORAK v. 40(2) APPLIED LIFE SCIENCES azido

2001-04-01

238

The importance of an accurate target wave function in variational calculations for (e^{+}-H_{2}) scattering

Several different aspects of the influence of the target backing on in-beam electron spectra following compound nuclear reactions induced by accelerated ions at tandem energies irradiating backed targets are discussed in detail. This discussion is illustrated by a few typical examples, such as "1"2C"5"+ and "3"1P"1"0"+ beams at 4 MeV/u bombarding Sn(+Be), Sn(+Au), Pb(+C) backed targets. Moreover, the relative influence of electron backscattering, electron Doppler shift and Doppler broadening as well as #delta#-electron emission on the low energy electron spectra (E_e#<=#100 keV) obtained under such conditions are investigated in the frame of the available experimental data. (orig.).

239

The Growth Cone Cytoskeleton in Axon Outgrowth and Guidance

Using the complex Kohn method, we have calculated variational values of phase shifts and the annihilation parameter, Z_{eff}, for the elastic scattering of positrons by molecular hydrogen. Our results are sensitive to small changes in the accuracy of the wave function representing the target hydrogen molecule. We have developed a systematic approach to demonstrate that, at low positron energies, there are particular forms of the Kohn trial wave function for which the results of variational calculations are not reliable, even when the target wave function accounts for as much as 96.8% of the correlation energy of H_{2}. We find that reliable results can be recovered if our calculations are extended to admit more sophisticated target wave functions accounting for 99.7% of the correlation energy. Remaining discrepancies between theory and experiment are briefly discussed.

2008-01-01

240

The Development of New Methods for Solving the Target ...

Axon outgrowth and guidance to the proper target requires the coordination of filamentous (F)-actin and microtubules (MTs), the dynamic cytoskeletal polymers that promote shape change and locomotion....Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Target space duality I: general theory

... and 7 survey papers. On the inverse Stefan problem he wrote 3 research papers and 1 survey paper. The papers on inverse ...

1984-07-18

242

Studies of relativistic heavy ion collisions at the AGS (Experiment 814)

We develop a systematic framework for studying target space duality at the classical level. We show that target space duality between manifolds M and M-tilde arises because of the existence of a very special symplectic manifold. This manifold locally looks like MxM-tilde and admits a double fibration. We analyze the local geometric requirements necessary for target space duality and prove that both manifolds must admit flat orthogonal connections. We show how abelian duality, nonabelian duality and Poisson-Lie duality are all special cases of a more general framework. As an example we exhibit new (nonlinear) dualities in the case M=M-tilde=R{sup n}.

2000-09-25

243

Smoking and reproduction: The oviduct as a target of cigarette smoke

This report discusses the experimental setup of experiment 814 at Brookhaven AGS. This experiment involves the collision of silicon ions with target nuclei. The detector systems are discussed primarily. (LSP)

1990-01-01

244

Simultaneous recognition and segmentation of cells: application in C.elegans

The oviduct is an exquisitely designed organ that functions in picking-up ovulated oocytes, transporting gametes in opposite directions to the site of fertilization, providing a suitable environment...Full Text Available

245

Principles of antibody therapy.

Motivation: Automatic recognition of cell identities is critical for quantitative measurement, targeting and manipulation of cells of model animals at single-cell resolution. It has been...Full Text Available

2011-10-15

246

Novel Cytotoxic Vectors Based on Adeno-Associated Virus

The success of monoclonal antibodies in clinical practice is dependent on good design. Finding a suitable target is the most important part as other properties of the antibody can be altered by genetic...Full Text Available

1992-12-05

247

Wastenet

positive primary PymT breast cancer cells in primary co-cultured tumor tissue, suggesting target specificity of

248

Measured Temperatures of Solid Rocket Motors Dump Stored ...

Knowledge-Based Identification of the ERK2/STAT3 Signal Pathway as a Therapeutic Target for Type 2 Diabetes and Drug Discovery

... TARGET SENSOR-MK 80 SERIES BOMBS ... 5-inch-, and 2.75-inch- diameter rocket motors that ... balance when compared to incoming solar radiation ...

1989-07-01

249

British Library Electronic Table of Contents (United Kingdom)

Many existing agents for diabetes therapy are unable to restore or maintain normal glucose homeostasis or prevent the eventual emergence of hyperglycemia-related complication. Therefore, agents based on novel mechanisms are sought to complement and extend the current therapeutic approaches. Based on the initial paper research, we focused on active STAT3 as an attractive pharmacological target for type 2 diabetes. The subsequent text mining with a unique query to identify suppressors but not activators of STAT3 revealed the ERK2/STAT3 pathway as a novel diabetes target. The description of ERK2 inhibitors as diabetes target had not been found in our text mining research at present. The mechanism-based peptide inhibitor for ERK2 was identified using the knowledge of the KIM sequence, which ha...

2011-01-01

250

Johnson News - NASA

Ion channels, transporters, and pumps as targets for heart failure

Nov 21, 2003... for the European Space Agency's Automated Transport Vehicle, a new, uncrewed station cargo vehicle targeted for launch late next year. ...

251

Imaging immune response in vivo

Congestive heart failure (CHF) is a leading cause of morbidity and mortality. CHF is marked by atrial and ventricular enlargement and reduced cardiac contractility, as well as an association...Full Text Available

2009-10-01

252

Heat shock proteins as emerging therapeutic targets

PurposeClinical trials have commenced to evaluate the feasibility of targeting malignant gliomas with genetically engineered cytolytic T-cells (CTLs) delivered directly...Full Text Available

2008-06-15

253

Genomics of human longevity

Chaperones (stress proteins) are essential proteins to help the formation and maintenance of the proper conformation of other proteins and to promote cell survival after a large variety of environmental...Full Text Available

2005-11-01

254

Gene therapy for ocular diseases

In animal models, single-gene mutations in genes involved in insulin/IGF and target of rapamycin signalling pathways extend lifespan to a considerable extent. The genetic, genomic and epigenetic influences...Full Text Available

2011-01-12

255

GAVA: Spectral Simulation for In Vivo MRS Applications

The eye is an easily accessible, highly compartmentalised and immune-privileged organ that offers unique advantages as a gene therapy target. Significant advancements have been made in understanding...Full Text Available

2011-05-01

256

Evaluation of phenylpiperazines as targeting agents for neuroblastoma.

An application that provides a flexible and easy to use interface to the GAMMA spectral simulation package is described that is targeted at investigations using in vivo MR spectroscopic methods....Full Text Available

2007-04-01

257

Effects of voicing in the recognition of concurrent syllables (L)a)

The potential of radiolabelled phenylpiperazines as agents for the detection and therapy of tumours of neural crest origin was evaluated by in vitro pharmacological studies with human neuroblastoma...Full Text Available

1996-09-01

258

Dynamic clamp with StdpC software

This letter reports a study designed to measure the benefits of voicing in the recognition of concurrent syllables. The target and distracter syllables were either voiced or whispered, producing...Full Text Available

2009-12-01

259

Dynamic Weapon-Target Assignment Problems with ...

Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based...Full Text Available

2011-03-01

260

Cooperative Charging Effects of Fibers From Electrospinning ...

... defense's weapons are Space-based kinetic-kill vehicles ... I ti I obtained by dividing the weapons as ... of the optimal weapon-arge assigntments and ...

1988-06-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Comparative genomics of insect juvenile hormone biosynthesis?

... In the Proceedings of INTC 2003, we studied charging effects on target coverage during electrospinning (16). ... of Fibers from Electrospinning of ...

2005-01-05

262

Cholinergic modulation of multivesicular release regulates striatal synaptic potency and integration

The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular...Full Text Available

2006-04-01

263

Bacterial flora-typing with targeted, chip-based Pyrosequencing

The pleiotropic actions of neuromodulators on pre- and postsynaptic targets present challenges to disentangling the mechanisms underlying regulation of synaptic transmission. Within the striatum,...Full Text Available

2009-09-01

264

Approaches to the evaluation of chemical-induced immunotoxicity.

BackgroundThe metagenomic analysis of microbial communities holds the potential to improve our understanding of the role of microbes in clinical conditions. Recent, dramatic improvements...Full Text Available

265

An Extended Kalman Filter for Use in a Shared Aperture ...

The immune system plays a crucial role in maintaining health; however, accumulating evidence indicates that this system can be the target for immunotoxic effects caused by a variety of chemicals including...Full Text Available

1995-12-01

266

A comprehensive assessment of N-terminal signal peptides prediction methods

... used to track a point source target ... A one sigma tracking error of .2 ... LOOKING INFRARED SYSTEMS, *KALMAN FILTERING, SIGNAL PROCESSING ...

1978-12-01

267

Testing of CFC Targets by Plasma Heat Fluxes Relevant to Elms and Mitigated Disruptions in ITER

BackgroundAmino-terminal signal peptides (SPs) are short regions that guide the targeting of secretory proteins to the correct subcellular compartments in the cell. They are cleaved...Full Text Available

268

Targeted suppression of Has2 mRNA in mouse cumulus cell-oocyte complexes by adenovirus-mediated short-hairpin RNA expression

Carbon fibre composite (CFC) was irradiated by hot plasma streams at plasma gun facility MK-200UG. The CFC targets were tested by plasma loads relevant to Edge Localized Modes (ELM) and mitigated disruptions in ITER. Onset condition of CFC evaporation and properties of evaporated carbon were studied by use of infrared pyrometry and visible spectroscopy.

2006-01-01

269

Targeted Capture and Next-Generation Sequencing Identifies C9orf75, Encoding Taperin, as the Mutated Gene in Nonsyndromic Deafness DFNB79

RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This...Full Text Available

2009-06-01

270

Structure functions at low Q^2: higher twists and target mass effects

Targeted genome capture combined with next-generation sequencing was used to analyze 2.9 Mb of the DFNB79 interval on chromosome 9q34.3, which includes 108 candidate genes. Genomic...Full Text Available

2010-03-12

271

Production of "2"0"3Pb by proton irradiation of Bi

We review the physics of structure functions at low Q{sup 2}, focusing on the phenomenon of quark-hadron duality and the resonance-scaling transition, both phenomenologically and in the context of quark models. We also present a new implementation of target mass corrections to nucleon structure functions which, unlike existing treatments, has the correct kinematic threshold behavior at finite Q{sup 2} in the x -> 1 limit.

2006-05-22

272

PV Conversion Technologies, Session: OPV, Sensitized, Seed (Presentation)

Production of lead-203 by proton irradiation of bismuth metal has been investigated as a replacement method for commercially available lead-203. Targets of bismuth metal (0.1 cm thick) were irradiated for periods of 1-3 hours with 90 MeV protons. After processing of the target, the estimated yield of lead-2-3 was 30 millicuries/microamp.

1990-06-24

273

Modeled Neutron Induced Nuclear Reaction Cross Sections for Radiochemistry in the region of Iridium and Gold

The NREL Sensitized Solar Cell (SSC) Core Program supports the Solar America Initiative by: (1) targeting new devices and processes for commercialization by 2015 that are less expensive, more efficient, highly reliable, and environmentally benign; (2) collaborating with DOE OS/BES to conduct basic research targeting breakthroughs in key areas, such as ultra-high efficiency and/or ultra-low cost materials and devices.

2008-04-01

274

Kidney-specific allo- and autoantibodies in the alloantibody response to rat kidney: the use of kidney homogenate as a target for serological analysis.

We have developed a set of modeled nuclear reaction cross sections for use in radiochemical diagnostics. Systematics for the input parameters required by the Hauser-Feshbach statistical model were developed and used to calculate neutron induced nuclear reaction cross sections for targets ranging from osmium (Z = 76) to gold (Z = 79). Of particular interest are the cross sections on Ir and Au including reactions on isomeric targets.

2008-02-01

275

In vitro atrazine-exposure inhibits human natural killer cell lytic granule release

LEW anti-DA kidney and DA anti-LEW kidney sera were assayed using an indirect 125I anti-immunoglobulin-binding assay with kidney homogenate as target. This allowed the full spectrum of antibodies to...Full Text Available

1980-04-01

276

Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or heteromeric artificial miRNA targeting AP1 and CAL genes

The herbicide atrazine is a known immunotoxicant and an inhibitor of human natural killer (NK) cell lytic function. The precise changes in NK cell lytic function following atrazine exposure have not been fully elucidated. The current study identifies the point at which atrazine exerts its affect on the stepwise process of human NK cell-mediated lyses of the K562 target cell line. Using intracellular staining of human peripheral blood lymphocytes, it was determined that a 24-h in vitro exposure to atrazine did not decrease the level of NK cell lytic proteins granzyme A, granzyme B or perforin. Thus, it was hypothesized that atrazine exposure was inhibiting the ability of the NK cells to bind to the target cell and subsequently inhibit the release of lytic protein from the NK cell. To test this hypothesis, flow cytometry and fluorescent microscopy were employed to analyze NK cell-target cell co-cultures following atrazine ...

2007-06-01

277

Electron accelerator unit for electron beam therapy

Gene silencing is a useful technique for elucidating biological function of genes by knocking down their expression. A recently developed artificial microRNAs (amiRNAs) exploits an endogenous...Full Text Available

2009-03-01

278

Electron accelerator unit for electron beam therapy

An electron accelerator unit is described for electron beam therapy, comprising: a source of an electron beam; means for finally directing at least a portion of the beam to a therapy site, the directing means being mechanically independent of, and electrically isolated from, the source, and having a target area; and means for aligning the source with the directing means, the aligning means comprising means for projecting at least one beam of light from the source toward the target area.

1987-01-27

279

Double-electron-capture cross section for I/sup +/ in a magnesium-vapor target

An electron accelerator unit is described for electron beam therapy, comprising: a source of an electron beam; means for finally directing at least a portion of the beam to a therapy site, the directing means being mechanically independent of, and electrically isolated from, the source, and having a target area; and means for aligning the source with the directing means, the aligning means comprising means for projecting at least one beam of light from the source toward the target area.

280

Development of High-Efficiency Low-Lift Vapor Compression System - Final Report

Measurements of the double-electron-capture process in which a positive ion of iodine becomes a negative ion in a single collision with a magnesium atom are reported between 20 and 90 keV. The cross section is comparable to that for the rare gases and not as large as might be expected from a two-valence-electron atom. This process is probably insignificant in the production of negative ion beams using a magnesium-vapor target.

1987-06-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

281

Designing Human m₁ Muscarinic Receptor-Targeted Hydrophobic Eigenmode Matched Peptides as Functional Modulators

PNNL, with cofunding from the Bonneville Power Administration (BPA) and Building Technologies Program, conducted a research and development activity targeted at addressing the energy efficiency goals targeted in the BPA roadmap. PNNL investigated an integrated heating, ventilation and air conditioning (HVAC) system option referred to as the low-lift cooling system that potentially offers an increase in HVAC energy performance relative to ASHRAE Standard 90.1-2004.

2010-03-31

282

Achieving cholesterol targets by individualizing starting doses of statin according to baseline low-density lipoprotein cholesterol and coronary artery disease risk category: The CANadians Achieve Cholesterol Targets Fast with Atorvastatin Stratified Titration (CanACTFAST) study

A new proprietary de novo peptide design technique generated ten 15-residue peptides targeting and containing the leading nontransmembrane hydrophobic autocorrelation wavelengths, “modes”,...Full Text Available

2004-03-01

283

A bioinformatics tool for linking gene expression profiling results with public databases of microRNA target predictions

BACKGROUND:Despite an increasing body of evidence on the benefit of lowering elevated levels of low-density lipoprotein cholesterol (LDL-C), there is still considerable concern that...Full Text Available

2010-02-01

284

Radiation inactivation target size of rat adipocyte glucose transporters in the plasma membrane and intracellular pools

MicroRNAs are short (∼22 nucleotides) noncoding RNAs that regulate the stability and translation of mRNA targets. A number of computational algorithms have been developed to help predict which...Full Text Available

2008-11-01

285

Proton G{sub E}/G{sub M} from beam-target asymmetry

The in situ assembly states of the glucose transport carrier protein in the plasma membrane and in the intracellular (microsomal) storage pool of rat adipocytes were assessed by studying radiation-induced inactivation of the D-glucose-sensitive cytochalasin B binding activities. High energy radiation inactivated the glucose-sensitive cytochalasin B binding of each of these membrane preparations by reducing the total number of the binding sites without affecting the dissociation constant. The reduction in total number of binding sites was analyzed as a function of radiation dose based on target theory, from which a radiation-sensitive mass (target size) was calculated. When the plasma membranes of insulin-treated adipocytes were used, a target size of approximately 58,000 daltons was obtained. For adipocyte microsomal membranes, we obtained target sizes of approximately 112,000 and 109,000 daltons prior ...

1987-06-15

286

One-class classifiers and their application to synthetic aperture radar target recognition

The ratio of the proton's electric to magnetic form factor, G{sub E}/G{sub M}, can be extracted in elastic electron-proton scattering by measuring cross sections, beam-target asymmetry, or recoil polarization. Separate determinations of G{sub E}/G{sub M} by cross sections and recoil polarization observables disagree for Q{sup 2}>1 (GeV/c){sup 2}. Measurement by a third technique might uncover an unknown systematic error in either of the previous measurements. The beam-target asymmetry has been measured for elastic electron-proton scattering at Q{sup 2} = 1.51 (GeV/c){sup 2} for target spin orientation aligned perpendicular to the beam momentum direction. This is the largest Q{sup 2} at which G{sub E}/G{sub M} has been determined by a beam-target asymmetry experiment. The result, {mu}G{sub E}/G{sub M}=0.884{+-}0.027{+-}0.029, is compared to previous world data.

2006-09-15

287

One-class classifier networks for target recognition applications

Target recognition requires the ability to distinguish targets from non-targets, a capability called one-class generalization. To function as a one-class classifier, a neural network must have three types of generalization: within-class, between-class, and out-of-class. We discuss these three types of generalization and identify neural network architectures that meet these requirements. We have applied our one-class classifier ideas to the problem of automatic target recognition in synthetic aperture radar. We have compared three neural network algorithms: Carpenter and Grossberg`s algorithmic version of the Adaptive Resonance Theory (ART-2A), Kohonen`s Learning Vector Quantization (LVQ), and Reilly and Cooper`s Restricted Columb Energy network (RCE). The ART 2-A neural network has given the best results, with 100% within-class, and out-of-class generalization. Experiments show that the network`s ...

1992-10-01

288

Integral cross sections of 50.5 MeV #alpha# particle inelastic scattering on 1p and (2s-1d) shell nuclei and scattering mechanisms

Target recognition requires the ability to distinguish targets from non-targets, a capability called one-class generalization. Many neural network pattern classifiers fail as one-class classifiers because they use open decision boundaries. To function as one-class classifier, a neural network must have three types of generalization: within-class, between-class, and out-of-class. We discuss these three types of generalization and identify neural network architectures that meet these requirements. We have applied our one-class classifier ideas to the problem of automatic target recognition in synthetic aperture radar. We have compared three neural network algorithms: Carpenter and Grossberg`s algorithmic version of the Adaptive Resonance Theory (ART-2A), Kohonen`s Learning Vector Quantization (LVQ), and Reilly and Cooper`s Restricted Coulomb Energy network (RCE). The ART 2-A neural network gives the best ...

1993-01-01

289

Donut-shaped high-dose configuration for proton beam radiation therapy

Obtained experimental data on integral cross sections (ICS) of inelastic scattering of 50.5 MeV #alpha# particles with the excitation of "6","7Li, "9Be, "1"2","1"3C, "1"4C, "1"4N, "2"0Ne, "2"4Mg, "2"8Si nucleus low-lying energy levels are discussed. Regularities, detected in the behaviour of ICS forward scattering for 20-90 deg angles and backscattering for 90-160 deg angles for the target-nucleus under investigation are considered. Effect of reaction open channel number on #alpha#-particle scattering ICS where n,p,d- and #alpha#-channels were considered as the main channels for all the target-nuclei, is discussed. Dependence of #alpha#-particle scattering ICS on the target-nucleus level excitation energy and dependences of reaction open channel number on the channel spin, calculated for 50.5 MeV #alpha# particles and different target nuclei are shown in the diagrams. It is noted that the observed ...

290

Donut-shaped high-dose configuration for proton beam radiation therapy

Background: the authors report on the conception and first clinical application of a donut-shaped high-dose configuration for proton therapy (PT). This approach allows one to intensify target volume dose coverage for targets encompassing a critical, dose-limiting structure - like here, the cauda equina -, whilst delivering minimal dose to other healthy structures surrounding the target, thereby reducing the integral dose. Methods and results: intensity-modulated PT methods (IMPT) for spot scanning were applied to create and deliver a donut-shaped high-dose configuration with protons, allowing treating > 75% of the target with at least 95% of the prescribed dose of 72.8 CGE, whilst restricting dose to the cauda equina to 60-65 CGE. Integral dose was lower by a factor of 3.3 as compared to intensity-modulated radiotherapy with photons (IMXT). Conclusion: IMPT and spot scanning technology allow a ...

2005-01-01

291

Radioactive targets for neutron-induced cross section measurements

Background: the authors report on the conception and first clinical application of a donut-shaped high-dose configuration for proton therapy (PT). This approach allows one to intensify target volume dose coverage for targets encompassing a critical, dose-limiting structure - like here, the cauda equina -, whilst delivering minimal dose to other healthy structures surrounding the target, thereby reducing the integral dose. Methods and results: intensity-modulated PT methods (IMPT) for spot scanning were applied to create and deliver a donut-shaped high-dose configuration with protons, allowing treating > 75% of the target with at least 95% of the prescribed dose of 72.8 CGE, whilst restricting dose to the cauda equina to 60-65 CGE. Integral dose was lower by a factor of 3.3 as compared to intensity-modulated radiotherapy with photons (IMXT). Conclusion: IMPT and spot scanning technology allow a ...

2005-01-01

292

Transforming growth factor-b induces nerve growth factor expression in pancreatic stellate cells by activation of the ALK-5 pathway

Measurements using radioactive targets are important for the determination of key reaction path ways associated with the synthesis of the elements in nuclear astrophysics (sprocess), advanced fuel cycle initiative (transmutation of radioactive waste), and stockpile stewardship. High precision capture cross-section measurements are needed to interpret observations, predict elemental or isotopical ratios, and unobserved abundances. There are two new detector systems that are presently being commissioned at Los Alamos National Laboratory for very precise measurements of (n,{gamma}) and (n,f) cross-sections using small quantities of radioactive samples. DANCE (Detector for Advanced Neutron-Capture Experiments), a 4 {pi} gamma array made up of 160 BaF{sub 2} detectors, is designed to measure neutron capture cross-sections of unstable nuclei in the low-energy range (thermal to {approx}500 keV). The high granularity and high detection efficiency of DANCE, combined with ...

2004-01-01

293

British Library Electronic Table of Contents (United Kingdom)

Nerve growth factor (NGF), a survival factor for neurons enforces pain by sensitizing nociceptors. Also in the pancreas, NGF was associated with pain and it can stimulate the proliferation of pancreatic cancer cells. Hepatic stellate cells (HSC) respond to NGF with apoptosis. Transforming growth factor (TGF)-b, one of the strongest pro-fibrogenic activators of pancreatic stellate cells (PSC) induced NGF and its two receptors in an immortalized human cell line (ihPSC) and primary rat PSC (prPSC) as determined by RT-PCR, western blot, and immunofluorescence. In contrast to HSC, PSC expressed both NGF receptors, although p75NTR expression was weak in prPSC. In contrast to ihPSC TGF-b activated both Smad signaling cascades in prPSC. NGF secretion was diminished by the activin-like kinase (ALK)...

2009-01-01

294

Transforming growth factor-b induces CD44 cleavage that promotes migration of MDA-MB-435s cells through the up-regulation of membrane type 1-matrix metalloproteinase

British Library Electronic Table of Contents (United Kingdom)

CD44, a transmembrane receptor for hyaluronic acid, is implicated in various adhesion-dependent cellular processes, including cell migration, tumor cell metastasis and invasion. Recent studies demonstrated that CD44 expressed in cancer cells can be proteolytically cleaved at the ectodomain by membrane type 1-matrix metalloproteinase (MT1-MMP) to form soluble CD44 and that CD44 cleavage plays a critical role in cancer cell migration. Here, we show that transforming growth factor-b (TGF-b), a multifunctional cytokine involved in cell proliferation, differentiation, migration and pathological processes, induces MT1-MMP expression in MDA-MB-435s cells. TGF-b-induced MT1-MMP expression was blocked by the specific extracellular regulated kinase-1/2 (ERK1/2) inhibitor PD98059 and the specific pho...

2009-01-01

295

Salicylate Treatment Improves Age-Associated Vascular Endothelial Dysfunction: Potential Role of Nuclear Factor kB and Forkhead Box O Phosphorylation

British Library Electronic Table of Contents (United Kingdom)

We hypothesized that I kappa B kinase (IKK)-mediated nuclear factor kappa B and forkhead BoxO3a phosphorylation will be associated with age-related endothelial dysfunction. Endothelium-dependent dilation and aortic protein expression/phosphorylation were determined in young and old male B6D2F1 mice and old mice treated with the IKK inhibitor, salicylate. IKK activation was greater in old mice and was associated with greater nitrotyrosine and cytokines. Endothelium-dependent dilation, nitric oxide (NO), and endothelial NO synthase phosphorylation were lower in old mice. Endothelium-dependent dilation and NO bioavailability were restored by a superoxide dismutase mimetic. Nuclear factor kappa B and forkhead BoxO3a phosphorylation were greater in old and were associated with increased express...

2011-01-01

296

Proteomic Analysis of Proteins Secreted by HepG2 Cells Treated with Butyl Benzyl Phthalate

British Library Electronic Table of Contents (United Kingdom)

Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 ?M) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate ...

2010-01-01

297

Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe

Loss of PINK1 function decreases PP2A activity and promotes autophagy in dopaminergic cells and a murine model

Investigations into sexual differentiation and pheromone response in the fission yeast Schizosaccharomyces pombe are complicated by the need to first starve the cells of nitrogen. Most mating-related experiments are therefore performed on non-dividing cells. Here we overcome this problem by using two mutants that bypass the nutritional requirements and respond to the M-factor mating pheromone in rich medium. The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP. When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state derepressed for pheromone-controlled functions. We now report that an h+ pat1-114 strain ...

1994-01-01

298

British Library Electronic Table of Contents (United Kingdom)

Parkinson's disease (PD) is the most common neurodegenerative movement disorder. Mutations in PTEN-induced kinase 1 (PINK1) are a frequent cause of recessive PD. Autophagy, a pathway for clearance of protein aggregates or impaired organelles, is a newly identified mechanism for PD development. However, it is still unclear what molecules regulate autophagy in PINK1-silenced cells. Here we report that autophagosome formation is promoted in the early phase in response to PINK1 gene silencing by lentivirus transfer vectors expressed in mouse striatum. Reduced PP2A activity and increased phosphorylation of PP2A at Y307 (inactive form of PP2A) were observed in PINK1-knockdown dopaminergic cells and striatum tissues. Treatment with C2-ceramide (an agonist of PP2A) reduced autophagy levels in PINK...

2011-01-01

299

Internal medical therapy of gastrointestinal stroma tumors; Internistische Therapie gastrointestinaler Stromatumoren

Increase in phorbol ester binding in liver microsomes after chronic administration of phenobarbital

Until recently no active treatment for advanced or metastatic gastrointestinal stroma tumors (GIST) was available. The tyrosine kinase inhibitor imatinib has revolutionized the treatment of this disease and the median overall survival now reaches 5 years. The standard dose of imatinib is 400 mg per day. Locally advanced GIST should be treated with systemic therapy prior to surgical resection. Imatinib was recently licensed for adjuvant therapy following complete surgical removal of GIST in patients with a significant risk of recurrence. (orig.) [German] Bis vor einigen Jahren existierte keine wirksame systemische Therapie fuer fortgeschrittene oder metastasierte gastrointestinale Stromatumoren (GIST). Der Tyrosinkinaseinhibitor Imatinib hat die Behandlung dieser Tumoren revolutioniert, die mediane Ueberlebenszeit liegt heute bei etwa 5 Jahren. Die Standarddosierung von Imatinib betraegt initial 400 mg/Tag. Lokal fortgeschrittene Tumoren werden primaer systemisch ...

2009-12-15

300

Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry

The effect of chronic administration of phenobarbital on the binding of phorbol-12,13-dibutyrate (({sup 3}H)PDBu), an activator of protein kinase C (PKC), was examined in rat liver microsomes. A significant increase in the number of binding sites was observed in microsomes of Fisher 344 rats. However, no change appeared in liver cytosol binding of PDBu. Consequently, a translocation process of PKC is unlikely. The increase in ({sup 3}H)PDBu binding in liver microsomes is significant 24 h. after one injection of phenobarbital and reaches its maximum in 2 days. In other strains of rats (ACI and lean Zucker), significant differences were found in the increase of ({sup 3}H)PDBu binding in microsomes. Fisher 344 were the most sensitive, lean Zucker rats, the least sensitive. Those results parallel the pentoxy-resorufin O demethylase activity in the microsomes of the same animals. EC{sub 50} values for inhibition of ({sup 3}H)PDBu binding by pentobarbital were determined ...

1991-03-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

British Library Electronic Table of Contents (United Kingdom)

We describe a method for the analysis of multi-site phosphorylation in serine/threonine (Ser/Thr)-rich protein sequences. Site-specific mutagenesis was used to introduce tryptic cleavage sites in the serine glutamine/threonine glutamine cluster domain (SCD) of the human checkpoint protein kinase (Chk2). The mutant proteins were shown to autophosphorylate on residues that are inducibly phosphorylated when mammalian cells are exposed to ionizing radiation (serine 33/35, serine 516, threonine 68 and threonine 432). Five Ser/Thr clusters within the SCD were flanked by arginine or lysine residues to produce tryptic peptides for nanospray liquid chromatography (nanoLC)/linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Phosphorylation sites were assigned usin...

2007-01-01

302

Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix

A novel small-molecule inhibitor of NF-#kappa#B signaling

Selecting an appropriate matrix solution is one of the most effective means of increasing the ionization efficiency of phosphopeptides in matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In this study, we systematically assessed matrix combinations of 2, 6-dihydroxyacetophenone (DHAP) and diammonium hydrogen citrate (DAHC), and demonstrated that the low ratio DHAP/DAHC matrix was more effective in enhancing the ionization of phosphopeptides. Low femtomole level of phosphopeptides from the tryptic digests of ?-casein and ?-casein was readily detected by MALDI-TOF-MS in both positive and negative ion mode without desalination or phosphopeptide enrichment. Compared with the DHB/PA matrix, the optimized DHAP/DAHC matrix yielded superior sample homogeneity and higher phosphopeptide measurement sensitivity, particularly when multiple phosphorylated peptides were assessed. Finally, the DHAP/DAHC matrix was applied to identify phosphorylation sites ...

2010-01-01

303

In vitro evaluation of herpes simplex virus type 1 thymidine kinase reporter system in dynamic studies of transcriptional gene regulation

The inducible transcription factor NF-#kappa#B regulates divergent signaling pathways including inflammatory response and cancer development. Selective inhibitors for NF-#kappa#B signaling are potentially useful for treatment of inflammation and cancer. NF-#kappa#B is canonically activated by preferential disposal of its inhibitory protein; I#kappa#B, which suppresses the nuclear translocation of NF-#kappa#B. I#kappa#B#alpha# (a major member of I#kappa#B family proteins) is phosphorylated with an I#kappa#B kinase (IKK) and subsequently polyubiquitylated by SCF"#beta#"T"r"C"P"1 ubiquitin-ligase in the presence of E1 and E2 prior to proteasomal degradation. Here, we describe a novel inhibitor termed GS143, which suppressed I#kappa#B#alpha# ubiquitylation, but not I#kappa#B#alpha# phosphorylation, MDM2-directed p53 ubiquitylation, and proteasome activity in vitro. GS143 markedly suppressed the destruction of I#kappa#B#alpha# stimulated by TNF#alpha# and a set of ...

2008-04-18

304

Simultaneous production of "2"8Mg and "4"7Ca by high-energy heavy-ion irradiation for applications in biology

The herpes simplex virus type 1 thymidine kinase (HSV1-TK) reporter system is being used to directly and indirectly monitor therapeutic gene expression, immune cell trafficking and protein-protein interactions in various living animals. However, the issues of HSV1-TK enzyme stability in living cells and whether this reporter system is optimal for dynamic studies of gene expression events in genetic imaging have not be addressed. The purpose of the present study was to evaluate the application of this reporter system in dynamic studies of transcriptional gene regulation. To achieve this purpose, we established two tetracycline-inducible murine sarcoma cell lines, tetracycline-turn-off HSV1-tk-expressing cell line (NG4TL4/tet-off-HSV1-tk) and tetracycline-turn-off Luc-expressing cell line (NG4TL4/tet-off-Luc), to create an artificially regulated gene expression model in vitro. The dynamic transcriptional events mediating a series of doxycycline (Dox) inductions were ...

2006-07-15

305

SiO{sub 2}-Ta{sub 2}O{sub 5} sputtering yields: simulated and experimental results

With the aim of preparing carrier-free "2"8Mg and "4"7Ca simultaneously, Ti, V and Fe targets were examined by irradiating with high-energy ions of "1"2C, "1"4N and "1"6O accelerated by the RIKEN ring cyclotron. Among the targets, V gave the highest cross section for the formation of both "2"8Mg and "4"7Ca irrespective of the kind of beams. The cross section for the formation of "2"8Mg by the reactions of Ti, V and Fe targets with ion beams increased in the order of "1"2C<"1"6O<"1"4N. On the other hand, the three beams exhibited almost the same cross sections for the formation of "4"7Ca by the reaction of a given target. Titanium and V were selected as prospective targets and "1"4N as a suitable beam for the production of "2"8Mg and "4"7Ca. Chemical separation procedures of the radiotracers in carrier- and salt-free states have been established by using cation exchange resins. ...

306

Measurement of K x-ray intensity ratio of tin, gadolinium and dysprosium

To improve mirrors coating, we have modeled sputtering of binary oxide targets using TRIM code. First, we have proposed a method to calculate TRIM input parameters using on the one hand thermodynamic cycle and on the other hand Malherbe`s results. Secondly, an iterative processing has provided for oxide steady targets caused by ionic bombardment. Thirdly, we have exposed a model to get experimental sputtering yields. Fourthly, for (Ar - SiO{sub 2}) pair, we have determined that steady target is a silica one. A good agreement between simulated and experimental yields versus ion incident angle has been found. For (Ar - Ta{sub 2} O{sub 5}) pair, we have to introduce preferential sputtering concept to explain discrepancy between simulation and experiment. In this case, steady target is tantalum monoxide. For (Ar - Ta+O{sub 2}) pair, tantalum sputtered by argon ions in reactive oxygen atmosphere, we have to ...

1994-09-01

307

Variational method for estimating the rate of convergence of Markov Chain Monte Carlo algorithms

Full text: Measurement of K_#beta# to K_#alpha# x-ray intensity ratios are important not only in the field of atomic physics, radiation physics and medical physics, but also to test the validity of assumptions made in the theoretical prediction. The intensity ratios can also give information on the effect of physical and chemical environment of the element in the compound. Many investigators have adopted a single and double reflection geometries to measure the K_#beta# to K_#alpha# x ray intensity ratios to understand the effect of physical and chemical environment on x-ray fluorescence. The targets are excited by a radioactive source of having activity of the order 100 MBq. in order to carry out accurate measurement K_#beta# to K_#alpha# x-ray intensity ratios, we have develop 2#pi# geometrical configuration method : placing a target right on the surface of the detector facing the target to measure the K shell fluorescence ...

2003-11-01

308

Transmission nuclear resonance fluorescence measurements of "2"3"8U in thick targets

We demonstrate the use of a variational method to determine a quantitative lower bound on the rate of convergence of Markov Chain Monte Carlo (MCMC) algorithms as a function of the target density and proposal density. The bound relies on approximating the second largest eigenvalue in the spectrum of the MCMC operator using a variational principle and the approach is applicable to problems with continuous state spaces. We apply the method to one dimensional examples with Gaussian and quartic target densities, and we contrast the performance of the basic Metropolis-Hastings algorithms with a ``smart'' variant that incorporates gradient information into the trial moves. We find that the variational method agrees quite closely with numerical simulations. We also see that the smart MCMC algorithm often fails to converge geometrically in the tails of the target density except in the simplest case we examine, and even then care ...

2006-01-01

309

The influence of chemically active gas on the light emission of metallic targets bombarded by positive ions

Transmission nuclear resonance fluorescence measurements were made on targets consisting of Pb and depleted U with total areal densities near 86g/cm"2. The "2"3"8U content in the targets varied from 0% to 8.5% (atom fraction). The experiment demonstrates the capability of using transmission measurements as a non-destructive technique to identify and quantify the presence of an isotope in samples with thicknesses comparable to the average thickness of a nuclear fuel assembly. The experimental data also appear to demonstrate the process of notch refilling with a predictable intensity. Comparison of measured spectra to previous backscatter "2"3"8U measurements indicates general agreement in observed excited states. Evidence of two new "2"3"8U excited states and possibly a third state have also been observed.

2011-05-15

310

Targeting the nicotinic alpha7 acetylcholine receptor to enhance cognition in disease

The introduction of oxygen in the vicinity of a metallic target surface, bombarded with positive argon ions of twenty kiloelectron-volts, increases the number of sputtered atoms in the excited state. This phenomenon of exaltation, very sensitive in the case of nickel and aluminium, is much less marked in the case of molybdenum. Moreover, the emission of excited particles coming from the beam's ions is not modified. A quantum-mechanical model of a kinetic emission process, which permits the interpretation of the clean metallic target's emission phenomena, seems insufficient to explain all of the results obtained in the presence of oxygen. In this last case one can therfore use a thermodynamic model in which excited metallic particles can be formed directly by chemical surface reactions of neutralization or reduction. (orig.).

311

British Library Electronic Table of Contents (United Kingdom)

A promising drug target currently under investigation to improve cognitive deficits in neuropsychiatric and neurological disorders is the neuronal nicotinic alpha7 acetylcholine receptor (a7nAChR). Improving cognitive impairments in diseases such as Alzheimer's (AD) and schizophrenia remains a large unmet medical need, and the a7nAChR has many properties that make it an attractive therapeutic target. The a7nAChR is a ligand gated ion channel that has particularly high permeability to Ca^2^+ and is expressed in key brain regions involved in cognitive processes (e.g., hippocampus). The a7nAChRs are localized both pre-synaptically, where they can regulate neurotransmitter release, and post-synaptically where they can activate intracellular signaling cascades and influence downstream processes...

2011-01-01

312

Targeted drug delivery under MRI guidance

British Library Electronic Table of Contents (United Kingdom)

The main goal of local drug delivery is to increase the concentration of a specific therapeutic agent in a target tissue with minimal nontarget distribution. Compared to systemic therapy, local drug delivery provides a high level of therapeutic efficacy with minimal systemic effects. The current primary imaging modality for drug delivery has been x-ray angiography, but it has major limitations including anatomical ambiguity and inability to visualize the targeted tissues. Due to these inherent problems, MR guidance has been explored as an alternative imaging modality for guiding and monitoring of drug therapy. Recently, interventional MR (XMR) systems have been implemented that have both dual x-ray and MRI capabilities in a single suite and allow for real-time interventional procedures to ...

2008-01-01

313

Study on possible fuel layering sequence for FIREX target

Studies on heterogeneous simulated Am targets. Influence of lanthanide oxides

A new procedure of fuel layering for the Fast Ignition Realization Experiment (FIREX) target is proposed. A conical laser guide heating technique was experimentally demonstrated in principle as the followings. It employed the target consisting of a polystyrene (PS) shell, a fill tube and a conical laser guide. At first, liquid fuel was fed into the shell and existed around the conical laser guide because the surface tension of the fuel must cause it. Then, it was solidified. The laser light provided a heat source to the conical laser guide so that the solid fuel was moved to the other interior of the shell. This process resulted in missing solid fuel around the conical laser guide. To fill the vacant space, liquid fuel was added as temperature was raised to the melting point. After the liquid fuel addition, temperature was lowered to the solidification point again. During this process, most of the solid fuel could survive.

2010-08-01

314

Studies of L x-rays from 64 MeV iodine projectiles in collision with gas targets

Targets for heterogeneous transmutation of americium are composed of a support material, the inert matrix and of an americium oxide compound: AmO{sub 2-x} or Am{sub 2}O{sub 3}. In parallel to the studies using americium, simulated compounds of americium oxide, chosen amongst lanthanide oxides, are used. The selection of these compounds is based on theoretical, physical and crystallochemical considerations. In order to assess the influence of impurities composed of lanthanide oxides, resulting from the separation process, on the physical and chemical properties of such a target, synthesis of oxide powders containing impurities associated with the simulated americium oxide, characterizations, crystallographic studies and sodium compatibility tests have been performed. (author)

1997-12-31

315

Some properties of atomic beam produced by laser induced ablation of Li target

We have studied L x-rays from 64 MeV iodine projectile in collision with various gas targets, Z_2 #18, do not arise from selective M subshell vacancy population, has been conclusively established by the observations by Datz et al (1971) and by Saha et al (1996) that the measured intensity ratio of the Ll and L#alpha# lines, which arise because of transitions from different M subshells into the same L, subshell, does not show any periodic behaviour with Z, but stays rather constant. Differences in the measured L#beta#_1/L#alpha# intensity ratio of iodine with 7"+ and 24"+ charge states impinging on Kr target established the minor role of the electrons in the N shell of the projectile in the x-ray production mechanism. (author)

1997-11-17

316

Separation of {sup 187,188}Pt and {sup 187,188}Ir produced in {sup 11}B{sup 4+} irradiated tantalum target

Pulsed atomic beams produced in vacuum by laser induced ablation from a lithium target are analyzed by laser induced fluorescence (LIF). The 1-mixing processes induced in the n = 9, 10 Li Rydberg states by collisions with CO_2 molecules illustrate the application of the method. Resolution is limited by the 1 mm diameter of the probe laser beam. Combining LIF and absorption measurements gives n_L_i as a function of time at various distances from the target surface. The investigation of the Li-C0_2 1-mixing process in a heat pipe oven proved impossible due to the high reactivity of Li with C0_2. This problem was solved by renewing the Li atoms at each laser shot. Values obtained for n = 9, n = 10 are k = 17 x 10"-"8 and 15 x 10"-"8 cc/sec, respectively.

317

Particle emission from low energy proton bombardment of TiH{sub 2} and TiD{sub 2}

Heavy ion irradiation on tantalum metal target with 57 MeV {sup 11}B{sup 4+} leads to the production of no-carrier-added radioisotopes of platinum, {sup 187,188}Pt and iridium, {sup 187,188}Ir in the matrix, which have been effectively separated from the bulk target and from each other with suitable anion exchangers employing liquid-liquid extraction (LLX). Also {sup 188,189}Pt and {sup 188,189}Ir radionuclides have been produced, from their short-lived precursors {sup 188,189}Au, in {sup 12}C{sup 6+} irradiated tantalum matrix. Gamma-spectroscopy has been utilised to determine the production, extent of separation and purity of the radiotracers at different stages of the experiment. (orig.)

2000-07-01

318

PIC Simulations Of Ion Acceleration By Linearly And Circularly Polarized Laser Pulses

TiH{sub 2} and TiD{sub 2} thick targets were bombarded with 100 to 200 keV protons. Evidence for nuclear reactions was obtained by means of a surface barrier particle detector. Proton irradiation of TiD{sub 2} produced the following observations: {alpha} particle emission identified as (p, {alpha}) reactions from {sup 11}B and {sup 7}Li impurities in the target at ppm concentrations; and {approx}3 MeV proton and {approx}1 MeV triton emission from secondary D-D reactions caused by elastic scattering of the primary proton with a target deuteron. A 3.9 MeV {alpha} particle peak measured by others was not observed. (author)

2002-03-01

319

Natural convection cooling of the IFMIF target and test cell

Linearly polarized laser radiation accelerates electrons to very high velocities and these electron form a sheath layer on the rear side of thin targets where preferentially protons are accelerated. When mass-limited targets are used, the lateral transport of the absorbed laser energy is reduced and the accelerating field is enhanced. For targets consisting of two ion species, heavier ions facilitate formation of quasi-monoenergetic bunch of lighter ions. For circularly polarized light, fast electron production is suppressed by the absence of the oscillatory component of the ponderomotive force. Ions are accelerated on the front side by the separation field and very thin foil can be accelerated as one massive quasi-neutral block. As all ion species acquire the same velocity, this acceleration mechanism is preferred for heavier ions.

2008-06-24

320

Inelastic excitation in the collisions of relativistic heavy ions

The present work summarizes efforts on the simulation of natural convection cooling within the IFMIF target and test cell. The simulations have been performed with the STAR-CD code using the k-#omega# high-Reynolds number turbulence model. A dedicated thermohydraulic model has been devised including Lithium loop components. Nuclear heat production has been calculated by the Monte-Carlo code McDeLicious for different parts of the target and test cell walls and was used as input for the STAR-CD simulations. Helium atmospheres at several pressures from 0.1 to 10"-"5 MPa have been investigated. In order to limit the maximum temperature of the concrete walls to 80 deg. C it was necessary to add thermal insulation layers to the hot Lithium loop surfaces and a conceptual system of two cooling layers in different depths of the concrete walls.

2007-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Improvement of tracking performance using prediction-based algorithms for a maneuvering target

The penumbra of the profile function describing the nuclear collisions of relativistic heavy ions is related to the cross section for their producing a specific target-nucleus #gamma#-ray, sigmasub(#gamma#). The analysis assumes that the diffuse-edge diffraction model suffices to calculate the inelastic cross sections to the particle-stable states of the target nucleus. When the deformation lengths, deltasub(L) = #beta#sub(L)R, characterizing all important excitations are available from other experiments and the #gamma#-branching of these states is also known, the only unknown quantity determining sigmasub(#gamma#) is found to be the ratio of the diffuseness parameter of the profile function, d, to its radius, R. The most reliably determined values of d/R, for the target "4"0Ca, imply a rather wide penumbra, consistent with microscopic calculations. (orig.).

322

British Library Electronic Table of Contents (United Kingdom)

This paper presents a study involving prediction of a complicated maneuvering target, with the aim of improving the tracking performance of a fire control system (FCS). In this study, we predict the position of a complicated maneuvering target 5 s in advance using the information up to the current time. Because of the large error caused by the complicated maneuvers and the long prediction time interval, the mechanical system of the fire control system will take a heavy load. In order to cope with this problem, several approaches to decreasing the prediction error have been proposed including the prediction algorithms based on the multiple model(MM) filter, interacting multiple model (IMM) filter, and variable dimension with input estimation (VDIE) filter. Finally, comparative simulation re...

2011-01-01

323

Hyperspectral remote sensing for mineral exploration in Pulang, Yunnan Province, China

British Library Electronic Table of Contents (United Kingdom)

The launch of the first spaceborne hyperspectral instrument, Hyperion, in 2000 has provoked further research into its capabilities with regard to mineral exploration. Our study in the remote, mountainous region of Pulang, China employed a two-step progressive approach, first to locate target areas characterized by hydrothermal mineral alteration, using the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), and secondly, to attempt detailed mineral mapping using Hyperion. The preliminary target detection involved principal components and broad-band spectral analysis and led to the detection of two target areas characterized by argillic alteration, iron-oxide- and sulphate-bearing minerals. A focused hyperspectral study followed using Spectral Angle Mapper (SAM) and Mixt...

2011-01-01

324

Detached divertor plasmas in JET

Analysis of neutron emission spectra for 30-50 MeV #alpha#-particle induced reactions in thick targets

In simulations with high radiated power fractions, it is possible to produce the drop in ion current to the divertor targets typical of detached plasmas. Despite the fact that these experiments are performed on beryllium target tiles, radiation from deuterium and beryllium cannot account for the measured power losses. The neutral deuterium levels in the SOL in these plasmas are higher than the model predicts. This may be due to leakage from the divertor or to additional wall sources related to the non-steady nature of these plasmas. In contrast, a surprisingly high level of carbon is present in these discharges; higher even than would be predicted are the divertor target tiles pure carbon. This level may well be large enough to produce the measured radiation. (authors). 6 refs., 2 figs., 1 tab.

1994-07-01

325

A combined numerical and theoretical study on the penetration of a jacketed rod into semi-infinite targets

Comparisons of calculated neutron yield distributions from #alpha#-particle induced reactions on thick targets are made with measured data to analyze the initial reaction process in the framework of the exciton (hybrid) model code ALICE91 (M. Blann, Lawrence Livermore National Laboratory Report UCID 19614, 1982). We have considered two reaction mechanisms: dissolution of the #alpha# in the nuclear field, and preequilibrium processes initiated by #alpha#-nucleon collisions. Both these processes seem to contribute to the emitted neutron spectra in varying proportions depending on the incident #alpha# energy and possibly on the target nucleus. Contributions from other processes appear to be non-negligible.

2003-06-01

326

British Library Electronic Table of Contents (United Kingdom)

A combined numerical and theoretical study is conducted herein on the penetration of semi-infinite targets by jacketed rods with different r"j"0/r"c"0 ratios where r"j"0 and r"c"0 are the radii of the jacket and the core, respectively. The numerical results show that for smaller r"j"0/r"c"0 ratios the u-v relationship changes only a little compared to that of unitary long rod penetrator of the same core material, hence, the u-v relationship of unitary (homogeneous) long rod penetration is also applicable for jacketed rod penetration. Model for cratering in semi-infinite targets by jacketed rods is then suggested by using the laws of conversation of mass, momentum and energy, together with the u-v relationship of unitary (homogeneous) long rod penetration and an analytical model for predict...

2011-01-01

327

What's new in target volume definition for radiologists in ICRU Report 71? How can the ICRU volume definitions be integrated in clinical practice?

Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space

AbstractThe optimal definition of the size, shape and location of gross tumour volume is one of the most important steps in the planning of radiation therapy, and necessitates a proper...Full Text Available

328

Update on the Role of Substantia Nigra Pars Reticulata in the Regulation of Seizures

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

2010-12-01

329

Three-dimensional simulation study of compact toroid plasmoid injection into magnetized plasmas

The substantia nigra pars reticulata (SNR) represents an endogenous seizure suppressing system, which may be targeted to develop treatments for generalized or multifocal epilepsies. This review summarizes...Full Text Available

2006-05-01

330

The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens

Three-dimensional dynamics of a compact toroid (CT) plasmoid, which is injected into a magnetized target plasma region is investigated by using magnetohydrodynamic (MHD) numerical simulations. It is found that the process of the CT penetration into this region is much more complicated than what has been analyzed so far by using a conducting sphere (CS) model. The injected CT suffers from a tilting instability, which grows with the similar time scale as the CT penetration. The instability is accompanied by magnetic reconnection between the CT magnetic field and the target magnetic field, which disrupts the magnetic configuration of the CT. Magnetic reconnection plays a role to supply the high density plasma initially confined in the CT magnetic field into the target region. Also, the penetration depth of the CT high density plasma is examined. It is shown to be shorter than that estimated from the CS model. The CT high ...

1999-04-01

331

The selective hypoxia inducible factor-1 inhibitor PX-478 provides in vivo radiosensitization through tumor stromal effects

BackgroundPCR amplification for the detection of pathogens in biological material is generally considered a rapid and informative diagnostic technique. Invasive Salmonella...Full Text Available

332

The selection and definition of targeted work-related conditions for surveillance under SENSOR.

Hypoxia inducible factor-1 (HIF-1) promotes tumor cell adaptation to microenvironmental stress. HIF-1 is up-regulated in irradiated tumors and serves as a promising target for radiosensitization....Full Text Available

2009-04-01

333

The prospects for polarized target materials with pure carbon background

Lists of reportable conditions and case definitions are important tools for epidemiologic surveillance. As part of an initiative to encourage occupational disease surveillance systems linked to intervention...Full Text Available

1989-12-01

334

The implication of Sir2 in replicative aging and senescence in Saccharomyces cerevisiae

None of the materials presently in common use for polarized proton targets has a pure carbon nuclear background. The alcohols and diols contain some oxygen, and the ammonia and amine-based materials contain nitrogen and/or other noncarbon species. In the latter cases the noncarbon nuclei are measurably polarized as a concomitant of the process used to polarize the hydrogen nuclei. The relative simplicity of a pure carbon background would be advantageous for most types of scattering experiments and perhaps crucial for some. In addition to simplifying the kinematics of background events, pure carbon is relatively easy to prepare as a dummy'' target for background subtraction. Also, in such a target material, [sup 13]C-enrichment would yield a clean polarized [sup 13]C material. In this note I explore the possibilities for such materials, touching upon only what I consider to be the ...

1992-10-06

335

The anxious wait: assessing the impact of patient accessible EHRs for breast cancer patients

The target of rapamycin (TOR) pathway regulates cell growth and aging in various organisms. In Saccharomyces cerevisiae, silent information regulator 2 (Sir2) modulates cellular senescence. Moreover,...Full Text Available

336

The Spectrum of Monogenic Autoinflammatory Syndromes: Understanding Disease Mechanisms and Use of Targeted Therapies

BackgroundPersonal health records (PHRs) provide patients with access to personal health information (PHI) and targeted education. The use of PHRs has the potential to improve a...Full Text Available

337

The Regulation of Aging and Longevity

Monogenic autoinflammatory diseases encompass a distinct and growing clinical entity of multisystem inflammatory diseases with known genetic defects in the innate immune system. The diseases...Full Text Available

2008-07-01

338

The Magdalena Ridge Observatory 2.4 m Telescope

p53 plays a critical role in tumor suppression. As a transcription factor, in response to stress signals, p53 regulates its target genes and initiates stress responses, including cell cycle arrest,...Full Text Available

2011-04-01

339

The Burden of Common Infectious Disease Syndromes at the Clinic and Household Level from Population-Based Surveillance in Rural and Urban Kenya

EOS Technologies has been commissioned to design and build a unique 2.4m astronomical telescope for the Magdalena Ridge Observatory. This telescope utilizes a high quality primary mirror and cell from a now decommissioned military application. This paper describes the project and gives an overview of the telescope design. The Magdalena Ridge Observatory (MRO) 2.4 meter telescope will be primarily utilized to observe, track, and characterize solar system astronomical targets, Earth satellites, space vehicles, and terrestrial military targets. The telescope's rapid tracking (slew rates are 10o/sec) will allow it to move to any target and acquire data within one minute of receipt of notice. In this way, the telescope will be used to capitalize on targets of opportunity that occur in asteroid studies (e.g., Near Earth Objects) and in astrophysics, such as gamma ray bursts and other transient phenomena. ...

2006-07-01

340

Targeting the p53 Pathway in Ewing Sarcoma

BackgroundCharacterizing infectious disease burden in Africa is important for prioritizing and targeting limited resources for curative and preventive services and monitoring the...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Targeting the Pentose Phosphate Pathway in Syndrome X-related Cardiovascular Complications

The p53 tumour suppressor plays a pivotal role in the prevention of oncogenic transformation. Cancers frequently evade the potent antitumour surveillance mechanisms of p53 through mutation of the TP53...Full Text Available

2011-01-01

342

Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase

Syndrome X is a combination or co-occurrence of several known cardiovascular risk factors (including central obesity, dyslipidemias, fatty liver disease, hyperinsulinemia, insulin resistance,...Full Text Available

2010-05-01

343

Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery

The development of new methods for gene addition to mammalian genomes is necessary to overcome the limitations of conventional genetic engineering strategies. Although a variety of DNA-modifying enzymes...Full Text Available

2011-09-01

344

Target organs and systems: methodologies to assess immune system function.

We investigate a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles...Full Text Available

2010-09-01

345

Systematics of average radiative width of heavy nuclides

Immunotoxicity encompasses both reduced and heightened immune function. Diverse chemicals can impair functioning of the immune system. Both monographs and books have been devoted to detailed descriptions...Full Text Available

1998-04-01

346

Synthetic substrates for measuring activity of autophagy proteases

Systematics of neutron capture radiative width were studied in the target element range from Th to Cm. Reduced radiative widths were analyzed with a simple radiative width formula based on E1 transition. Average radiative width is presented with the standard deviation of 15%. (author)

1999-03-01

347

Synthesis and characterization of a biotinylated organophosphorus ester for detection and affinity purification of a brain serine esterase: neuropathy target esterase.

Atg4 cysteine proteases (autophagins) play crucial roles in autophagy by proteolytic activation of Atg8 paralogs for targeting to autophagic vesicles by lipid conjugation, as well as in subsequent deconjugation...Full Text Available

2010-10-01

348

Structure of Natural Killer Cell Receptor KLRG1 Bound to E-Cadherin Reveals Basis for MHC-Independent Missing Self Recognition

We have synthesized a novel stable precursor, saligenin phosphorotrichloridate, which, on reaction with N-monobiotinyldiamines, generates a series of biotinylated covalent inhibitors of serine esterases....Full Text Available

1994-07-15

349

Stem cell niches and other factors that influence the sensitivity of bone marrow to radiation-induced bone cancer and leukaemia in children and adults

SUMMARYThe cytolytic activity of natural killer (NK) cells is regulated by inhibitory receptors that detect the absence of self molecules on target cells. Structural studies of...Full Text Available

2009-07-17

350

Species-specific microRNA roles elucidated following astrocyte activation

Purpose: This paper reviews and reassesses the internationally accepted niches or ‘targets’ in bone marrow that are sensitive to the induction of leukaemia and primary...Full Text Available

2011-04-01

351

Soluble Epoxide Hydrolase as a Therapeutic Target for Cardiovascular Diseases

MicroRNAs (miRNAs) are short non-coding RNAs that play a central role in regulation of gene expression by binding to target genes. Many miRNAs were associated with the function of the central nervous...Full Text Available

2011-05-01

352

Soluble Epoxide Hydrolase Inhibition: Targeting Multiple Mechanisms of Ischemic Brain Injury with a Single Agent

PrefaceCardiovascular effects of epoxyeicosatrienoic acids (EETs) include vasodilation, vascular smooth muscle cell anti-migratory actions, and anti-inflammatory actions. These...Full Text Available

2009-10-01

353

Roles of lead-binding proteins in mediating lead bioavailability.

SummarySoluble epoxide hydrolase (sEH) is a key enzyme in the metabolic conversion and degradation of P450 eicosanoids called epoxyeicosatrienoic acids (EETs). Genetic variations...Full Text Available

2009-03-01

354

Role of nitroso radicals as drug targets in circulatory shock

The intracellular bioavailability of lead (Pb) at low dosage levels in major target organs such as the kidney and brain appears to be largely determined by complexation with a group of low molecular...Full Text Available

1998-12-01

355

Regional Convection-Enhanced Delivery of Gadolinium-labeled Albumin in the Rat Hippocampus In Vivo

A vast amount of circumstantial evidence implicates oxygen-derived free radicals (especially, superoxide and hydroxyl radical) and high-energy oxidants [such as peroxynitrite (OONO⁻)]...Full Text Available

2009-06-01

356

Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5_Hc Gene by Allelic Replacement

Convection-enhanced delivery (CED) has emerged as a promising method of targeted drug-delivery for treating central nervous system (CNS) disorders, but the influence of brain structure on infusate...Full Text Available

2010-03-15

357

Rapid, sensitive detection of Mycoplasma pneumoniae in simulated clinical specimens by DNA amplification.

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5...Full Text Available

1998-10-01

358

ROCK DEFORMATION. Final Progress Report

The polymerase chain reaction (PCR) was investigated as a means of diagnosing Mycoplasma pneumoniae infections. The target DNA sequence was a 375-bp segment of the P1 virulence protein. This DNA segment...Full Text Available

1992-12-01

359

RNA:DNA Ratio and Other Nucleic Acid Derived Indices in Marine Ecology

The Gordon Research Conference (GRC) on ROCK DEFORMATION was held at II Ciocco from 5/19/02 thru 5/24/02. Emphasis was placed on current unpublished research and discussion of the future target areas in this field.

2002-05-24

360

Putative Biomarkers and Targets of Estrogen Receptor Negative Human Breast Cancer

Some of most used indicators in marine ecology are nucleic acid-derived indices. They can be divided by target levels in three groups: 1) at the organism level as ecophysiologic indicators, indicators...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Protein Targets of Reactive Metabolites of Thiobenzamide in Rat Liver In Vivo

Breast cancer is a progressive and potentially fatal disease that affects women of all ages. Like all progressive diseases, early and reliable diagnosis is the key for successful treatment and annihilation....Full Text Available

362

Protein Damage by Reactive Electrophiles: Targets and Consequences

Thiobenzamide (TB) is a potent hepatotoxin in rats, causing dose-dependent hyperbilirubinemia, steatosis, and centrolobular necrosis. These effects arise subsequent to and appear to result from...Full Text Available

2008-07-01

363

Prolonged signaling at the parathyroid hormone receptor by peptide ligands targeted to a specific receptor conformation

It has been sixty years since the Millers first described the covalent binding of carcinogens to tissue proteins. Protein covalent binding was gradually overshadowed by the emergence of DNA...Full Text Available

2008-01-01

364

Preparation of a "6"2Zn-"6"2Cu generator and of "6"1Cu following alpha particle irradiation of a nickel target

The parathyroid hormone receptor (PTHR) is a class B G protein-coupled receptor that plays critical roles in bone and mineral ion metabolism. Ligand binding to the PTHR involves interactions to both...Full Text Available

2008-10-28

365

Potential of mTOR inhibitors for the treatment of subependymal giant cell astrocytomas in tuberous sclerosis complex

The "6"2Zn-"6"2Cu generator system is one of the few which yield a pure positron emitting daughter. The authors have developed a method for the preparation of this generator system following 32 MeV #alpha# particle bombardment of nickel targets. The chemical processing involves two stages: first a hydrous zirconium oxide column is used at pH 4-5 to separate the zinc and copper activities produced in the target nickel, and secondly, a conventional anion exchange column is used to retain the "6"2Zn and remove all copper activities in 2M HCl. This column serves as the "6"2Cu generator which will yield pure "6"2Cu repeatedly, free from "6"2Zn and other impurities, in a mixture of HCl and NaCl. A method is outlined also to obtain a solution of "6"2Zn using the hydrous zirconium oxide (HZO) column. "6"1Cu can be prepared as a by-product in the above procedure, but a method for the preparation of "6"1Cu alone using an 18 MeV #alpha# particle beam on a ...

366

Potassium channels as a potential therapeutic target for trigeminal neuropathic and inflammatory pain

Rapamycin inhibits the mTOR (target of rapamycin) pathway and extends lifespan in multiple species. The tuberous sclerosis complex (TSC) protein is a negative regulator of mTOR. In humans, loss of the...Full Text Available

367

Previous studies in several different trigeminal nerve injury/inflammation models indicated that the hyperexcitability of primary afferent neurons contributes to the pain pathway underlying mechanical...Full Text Available

368

Pion-induced fission

Paradoxical function for the receptor for advanced glycation end products in mouse models of pulmonary fibrosis

The A(..pi../sup +/,/sup 3/He)B reaction near threshold is studied in a model where the pion is absorbed by an /sup 4/He constituent of the target nucleus. The predictions of this model using harmonic oscillator cluster wave functions agree semi-quantitatively with the experimental data on the inverse reaction.

1982-03-10

369

Pancreatic cancer: molecular pathogenesis and new therapeutic targets

Idiopathic pulmonary fibrosis (IPF) is a progressive disease with poor survival. The identification of therapeutic targets is essential to improving outcomes. Previous studies found that expression...Full Text Available

2011-03-31

370

PCR-based method for targeting 16S-23S rRNA intergenic spacer regions among Vibrio species

Patients with pancreatic cancer normally present with advanced disease that is lethal and notoriously difficult to treat. Survival has not improved dramatically, despite routine use of chemotherapy...Full Text Available

2009-07-01

371

Optimization of isotope production by cross section determination

BackgroundThe genus Vibrio is a diverse group of Gram-negative bacteria comprised of 74 species. Furthermore, the genus has and is expected to continue expanding...Full Text Available

372

Optimal bone strength and mineralization requires the type 2 iodothyronine deiodinase in osteoblasts

In order to optimize the production of "2"0"1Tl and "6"7Ga using the (p, xn) reactions on enriched "2"0"3Tl and "6"8Zn targets we established a new set of cross section curves for the most relevant reactions. Target stacks made of up to 15 thin (50 #mu#m) natural Tl or Zn foils interleaved with brass degradation foils were irradiated with incident proton energies from 42 to 10 MeV in overlapping experiments. Yields for "2"0"0","2"0"1","2"0"2"m","2"0"3","2"0"4"mPB and "6"6","6"7","6"8Ga were computed from measured #gamma# emission rates. Where possible, cross section curves for the nuclear reactions involved were computed. Thick target yields and contamination ratios for enriched targets were derived and show good agreement with results from actual production runs. (orig.).

373

Nucleoside Drugs Induce Cellular Differentiation by Caspase-Dependent Degradation of Stem Cell Factors

Hypothyroidism and thyrotoxicosis are each associated with an increased risk of fracture. Although thyroxine (T4) is the predominant circulating thyroid hormone, target cell responses are determined...Full Text Available

2010-04-20

374

Noradrenergic ?₁ Receptors as a Novel Target for the Treatment of Nicotine Addiction

BackgroundStem cell characteristics are an important feature of human cancer cells and play a major role in the therapy resistance of tumours. Strategies to target cancer stem cells...Full Text Available

375

Nano to micro delivery systems: targeting angiogenesis in brain tumors

Nicotine is the main psychoactive ingredient in tobacco and its rewarding effects are considered primarily responsible for persistent tobacco smoking and relapse. Although dopamine has been extensively...Full Text Available

2010-07-01

376

Multiple Functions of the 37/67-kd Laminin Receptor Make It a Suitable Target for Novel Cancer Gene Therapy

Treating brain tumors using inhibitors of angiogenesis is extensively researched and tested in clinical trials. Although anti-angiogenic treatment holds a great potential for treating primary and secondary...Full Text Available

377

Motion compensation with a scanned ion beam: a technical feasibility study

The 37/67-kd laminin receptor, LAMR, is a multifunctional protein that associates with the 40S ribosomal subunit and also localizes to the cell membrane to interact with the extracellular matrix. LAMR...Full Text Available

2010-01-01

378

Molecular dynamics of a ?B DNA element: base flipping via cross-strand intercalative stacking in a microsecond-scale simulation

BackgroundIntrafractional motion results in local over- and under-dosage in particle therapy with a scanned beam. Scanned beam delivery offers the possibility to compensate target...Full Text Available

379

MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms

The sequence-dependent structural variability and conformational dynamics of DNA play pivotal roles in many biological milieus, such as in the site-specific binding of transcription factors to target...Full Text Available

2008-09-01

380

Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines

MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences....Full Text Available

2003-08-19

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Measurements of Spectral Characteristics of Swimmer Targets ...

BackgroundCD26 (dipeptidyl peptidase IV, DPPIV) is a 110 kDa surface glycoprotein expressed in most normal tissues, and is a potential novel therapeutic target for selected cancers....Full Text Available

382

Materials considerations for the National Spallation Neutron Source target

... 3.0 Radar Measurements 3-1 3. 1 Spectral Density Measurements 3-2 3. 1. 1 System Calibration 3-3 ... 3. 1 SPECTRAL DENSITY MEASUREMENTS ...

1974-05-01

383

Mass Production of 64Cu with 64Ni(p,n)64Cu Nuclear Reaction and Target Material Recycling

The National Spallation Neutron Source (NSNS), in which neutrons are generated by bombarding a liquid mercury target with 1 GeV protons, will place extraordinary demands on materials performance. The target structural material will operate in an aggressive environment, subject to intense fluxes of high energy protons, neutrons, and other particles, while exposed to liquid mercury and to water. Components that require special consideration include the Hg liquid target container and protective shroud, beam windows, support structures, moderator containers, and beam tubes. In response to these demands a materials R and D program has been developed for the NSNS that includes: selection of materials; calculations of radiation damage; irradiations, post irradiation testing, and characterization; compatibility testing and characterization; design and implementation of a plan for monitoring of materials performance in service; and ...

1997-08-01

384

Mammalian target of rapamycin signaling modulates photic entrainment of the suprachiasmatic circadian clock

64Cu (T1/2=12.7h, ?- decay: 40%, ?+ decay: 19%, E.C. decay: 41%) is one of the most useful radioisotope in the nuclear medicine due to its multiple decay mode and the intermediate half-life. Several nuclear reaction, i.e., 64Ni(p,n)64Cu, 68Zn(p,?n)64Cu and 64Ni(d,2n)64Cu have been investigated for production. The highest could be obtained with proton irradiation on the enriched 64Ni target. For mass and routine production, the 64Ni target fabrication using electroplating, the reliable chemical separation of 64Cu from the irradiated 64Ni target and the effective recovery process for the recycling of very expensive enriched material ($20,000/g) and so forth are absolutely necessary to be established. In this work, we report our mass production method of 64Cu with 64Ni and Cyclone-30 accelerator

2009-05-01

385

Linking Gene Expression and Functional Network Data in Human Heart Failure

Inducible gene expression appears to be an essential event that couples light to entrainment of the master mammalian circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus....Full Text Available

2010-05-05

386

LYN is a mediator of epithelial-mesenchymal transition and target of dasatinib in breast cancer

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

387

Kinetic Complexity of the Global Response to Glucocorticoid Receptor Action

Epithelial-mesenchymal transition (EMT), a switch of polarized epithelial cells to a migratory, fibroblastoid phenotype, is considered a key process driving tumor cell invasiveness and metastasis....Full Text Available

2010-03-15

388

Key Beliefs for Targeted Interventions to Increase Physical Activity in Children: Analyzing Data from an Extended Version of the Theory of Planned Behaviour

We have characterized the kinetic response of gene targets throughout the murine genome to transcriptional modulation by the glucocorticoid receptor (GR). In contrast to a model in which multiple genes...Full Text Available

2009-04-01

389

Intracellular delivery of an antisense?oligonucleotide via endocytosis of a G protein-coupled receptor

Given the high prevalence of overweight and low levels of physical activity among children, a better understanding of physical activity behaviour is an important step in intervention planning. This...Full Text Available

2010-01-01

390

Inhibition of lactate dehydrogenase A induces oxidative stress and inhibits tumor progression

Gastrin-releasing peptide receptor (GRPR), a member of the G protein-coupled receptor superfamily, has been utilized for receptor-mediated targeting of imaging and therapeutic agents; here we extend...Full Text Available

2010-10-01

391

Increased degradation of MYPT1 contributes to the development of tolerance to nitric oxide in porcine pulmonary artery

As the result of genetic alterations and tumor hypoxia, many cancer cells avidly take up glucose and generate lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc...Full Text Available

2010-02-02

392

In vivo expression and mitochondrial targeting of yeast apoiso-1-cytochrome c fusion proteins.

Myosin phosphatase target subunit 1 (MYPT1) is the regulatory subunit of myosin light chain phosphatase (MLCP). It plays a critical role in vasodilatation induced by cGMP-elevating agents such as nitric...Full Text Available

2010-07-01

393

In vitro analysis of nuclear mRNA export using molecular beacons for target detection

To define the import pathway for apoiso-1-cytochrome c in vivo, the coding region for bacterial chloramphenicol acetyltransferase (CAT) or yeast copper metallothionein (CuMT) was fused to the carboxy...Full Text Available

1990-11-01

394

Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an Avena sativa Plasma Membrane Determinant 1

A detailed molecular characterization of nuclear mRNA export will require an in vitro system, allowing a biochemical reconstitution of transport. To this end, an mRNA export assay has...Full Text Available

2003-06-01

395

Identification of circulating neuropilin-1 and dose-dependent elevation following anti-neuropilin-1 antibody administration

An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target...Full Text Available

1992-01-01

396

Identification of Achaete-scute complex-like 1 (ASCL1) target genes and evaluation of DKK1 and TPH1 expression in pancreatic endocrine tumours

Neuropilin-1 (NRP1) acts as a co-receptor for class 3 semaphorins and vascular endothelial growth factor and is an attractive angiogenesis target for cancer therapy. In addition to the transmembrane...Full Text Available

2009-07-01

397

Identification and Characterization of Retinoid-Active Short-Chain Dehydrogenases/Reductases in Drosophila melanogaster

BackgroundASCL1 role in pancreatic endocrine tumourigenesis has not been established. Recently it was suggested that ASCL1 negatively controls expression of the...Full Text Available

398

INVERTEBRATE FAUNA ASSOCIATED WITH TORPEDOGRASS, PANICUM REPENS (CYPERALES: POACEAE), IN LAKE OKEECHOBEE, FLORIDA, AND ...

BackgroundIn chordates, retinoid metabolism is an important target of short-chain dehydrogenases/reductases (SDRs). It is not known whether SDRs play a role in retinoid...Full Text Available

2009-10-01

399

Human Immunodeficiency Virus-1 Inhibition of Immunoamphisomes in Dendritic Cells Impairs Early Innate and Adaptive Immune Responses

... 154. By way of comparison, the aquatic weed Hygrophila polysperma (Roxb.) T. Anderson, a potential target for biological ... L. Sutton. 2000. Is the aquatic weed hygrophila, Hygrophila polysperma (Polemon...

400

Heat Stress Enhances the Accumulation of Polyadenylated Mitochondrial Transcripts in Arabidopsis thaliana

SUMMARYDendritic cells (DCs) in mucosal surfaces are early targets for human immunodeficiency virus-1 (HIV-1). DCs mount rapid and robust immune responses upon pathogen encounter....Full Text Available

2010-05-28

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Health and function of participants in the Long Life Family Study: A comparison with other cohorts

BackgroundPolyadenylation of RNA has a decisive influence on RNA stability. Depending on the organisms or subcellular compartment, it either enhances transcript stability or targets...Full Text Available

402

Hair Mercury Negatively Correlates with Calcium Pump Activity in Human Term Newborns and Their Mothers at Delivery

Individuals from families recruited for the Long Life Family Study (LLFS) (n= 4559) were examined and compared to individuals from other cohorts to determine whether the recruitment targeting longevity...Full Text Available

403

Gyrase B Inhibitor Impairs HIV-1 Replication by Targeting Hsp90 and the Capsid Protein*

BackgroundCalcium homeostasis is a known target of several environmental toxicants including lead and mercury.ObjectiveOur goal was to determine...Full Text Available

2008-02-01

404

Goldin says NASA must reach streamlining targets - Johnson Space ...

Chemical genetics is an emerging approach to investigate the biology of host-pathogen interactions. We screened several inhibitors of ATP-dependent DNA motors and detected the gyrase B inhibitor coumermycin...Full Text Available

2010-12-10

405

Glutamatergic regulation of ghrelin-induced activation of the mesolimbic dopamine system

Jan 20, 1995 ... Jenn-Air dowedraft cooktop, staintess steel, coil. $75; telephone ans mach, $25. Eric, x31917. $295; President & First Lady premier plus ...

406

Genetic Diversity in Blastomyces dermatitidis: Implications for PCR Detection in Clinical and Environmental Samples

Recently, we demonstrated that the central ghrelin signalling system, involving the ghrelin receptor (GHS-R1A), is important for alcohol reinforcement. Ghrelin targets a key mesolimbic circuit involved...Full Text Available

2011-01-01

407

Gene expression analysis of interferon ? in laser capture microdissected cervical epithelium

SummaryBlastomycosis is a serious and potentially fatal infection by the thermally dimorphic fungus Blastomyces dermatitidis. PCR assays targeting the BAD-1 virulence...Full Text Available

2010-03-01

408

Fyn Is a Novel Target of (?)-Epigallocatechin Gallate in the Inhibition of JB6 Cl41 Cell Transformation

Optimal sample handling techniques for tissue preparation and storage, RNA extraction and quantification, and target gene detection are crucial for reliable gene expression analysis. Methods...Full Text Available

2008-10-01

409

Forecasting changes in amphibian biodiversity: aiming at a moving target

The cancer preventive action of (−)-epigallocatechin gallate (EGCG), found in green tea, is strongly supported by epidemiology and laboratory research data. However, the mechanism by...Full Text Available

2008-03-01

410

Food Advertising and Marketing Directed at Children and Adolescents in the US

Amphibian population declines and sudden species' extinctions began to be noted at the beginning of the 1980s. Understanding the causes of the losses is hampered by our poor knowledge of the amphibian...Full Text Available

2005-02-28

411

First line targeted therapies in breast cancer: focus on bevacizumab

In recent years, the food and beverage industry in the US has viewed children and adolescents as a major market force. As a result, children and adolescents are now the target of intense and specialized...Full Text Available

412

Excitation functions for proton-induced reactions on natural hafnium: Production of {sup 177}Lu for medical use

The heterogeneity of metastatic breast cancer mandates the need to select therapies taking into account tumor and patient characteristics. Chemotherapy is indicated in the palliative setting especially...Full Text Available

2007-03-01

413

Excitation functions for proton-induced reactions on natural hafnium: Production of "1"7"7Lu for medical use

There is an increasing interest in using radioisotopes of rare earth elements for internal radiotherapy and for imaging in nuclear medicine. {sup 177}Lu is one of the promising radionuclides. This article reports on the first measurements of the excitation function for the production of {sup 177}Lu with proton-beam energies up to 17 MeV on natural hafnium targets. The experimental cross sections for the reaction {sup nat}Hf(p,x){sup 177}Lu were obtained by the activation of a stacked-foil target and subsequent gamma spectrometry. Theoretical cross sections were calculated up to 35 MeV with the EMPIRE nuclear reaction model code. The measured and calculated cross sections were used for deriving the thick-target yields and for estimating the production of other nuclides than {sup 177}Lu. Measured production cross sections of {sup 175,176,177,178}Ta on the same target are also presented.

2009-11-15

414

Examining the Phonological Neighborhood Density Effect Using Near Infrared Spectroscopy

There is an increasing interest in using radioisotopes of rare earth elements for internal radiotherapy and for imaging in nuclear medicine. "1"7"7Lu is one of the promising radionuclides. This article reports on the first measurements of the excitation function for the production of "1"7"7Lu with proton-beam energies up to 17 MeV on natural hafnium targets. The experimental cross sections for the reaction "n"a"tHf(p,x)"1"7"7Lu were obtained by the activation of a stacked-foil target and subsequent gamma spectrometry. Theoretical cross sections were calculated up to 35 MeV with the EMPIRE nuclear reaction model code. The measured and calculated cross sections were used for deriving the thick-target yields and for estimating the production of other nuclides than "1"7"7Lu. Measured production cross sections of "1"7"5","1"7"6","1"7"7","1"7"8Ta on the same target are also presented.

2009-11-01

415

Evidence for divided automatic attention

Phonological density refers to the number of words that can be generated by replacing a phoneme in a target word with another phoneme in the same position. Although the precise nature of the...Full Text Available

2011-09-01

416

Scientific Electronic Library Online (English)

Abstract in english A long-standing debate in the literature is whether attention can form two or more independent spatial foci in addition to the well-known unique spatial focus. There is evidence that voluntary visual attention divides in space. The possibility that this also occurs for automatic visual attention was investigated here. Thirty-six female volunteers were tested. In each trial, a prime stimulus was presented in the left or right visual hemifield. This stimulus was characteriz (more) ed by the blinking of a superior, middle or inferior ring, the blinking of all these rings, or the blinking of the superior and inferior rings. A target stimulus to which the volunteer should respond with the same side hand or a target stimulus to which she should not respond was presented 100 ms later in a primed location, a location between two primed locations or a location in the contralateral hemifield. Reaction time to the positive ...

2008-02-01

417

Evidence for an association between TSH and IGF-1 receptors

Evaluation of heterogeneity effects for Am reaction rates of the moderated subassemblies

TSH receptor (TSHR) plays a central role in regulating thyroid function and is targeted by IgGs in Graves’ disease (GD-IgG). Whether TSHR is involved in the pathogenesis of thyroid associated...Full Text Available

2008-09-15

418

Emulsified Nanoparticles Containing Inactivated Influenza Virus and CpG Oligodeoxynucleotides Critically Influences the Host Immune Responses in Mice

The technology of minor actinide recycling in fast reactors has been discussed. In CEA, the feasibility study on Am once-through recycling in Phenix core with the moderated target subassemblies (S/As) has been performed. In this report, the evaluation of the heterogeneity effect on the moderated target S/As is described. It was evaluated by the calculation with the continuous energy Monte-Carlo code, MVP, because the accuracy of the deterministic method for the moderated target S/As is unknown. The reaction rates of four isotopes (Am-241, Am-242, Am242m and Am-243) calculated with the heterogeneous model and the homogeneous one were compared. These four isotopes play the important role in Am incineration. It is seen that the homogeneous model overestimates the reaction rates of Am-241 and Am-243 by 4 - 13%, and underestimates those of Am-242 and Am-242m by 13 - 23%. Further investigation made it clear that the ...

1998-10-01

419

Elastic scattering of electrons from the N = 50 isotones: "8"8Sr, "8"9Y, "9"0Zr and "9"2Mo

BackgroundAntigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy...Full Text Available

420

Effects of local texture and grain structure on the sputtering performance of tantalum

... mev range 100-1000 molybdenum 92 molybdenum 92 target nuclear radii

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Dose Optimization for Long-term rAAV-mediated RNA Interference in the Nigrostriatal Projection Neurons

Tantalum and tantalum-based thin films have gained precedence as the diffusion barrier for copper interconnects used in the latest generation of integrated circuits (ICs). The paper presents insight and observations on the covariance of texture and grain size of wrought tantalum sputtering targets and their influence on sputtering performance. Previous studies involving deposition trials of tantalum targets of varying metallurgical character had demonstrated that both grain size and textural homogeneity is critical for assuring reliable sputtering performance of tantalum. Subsequently, a model had been proposed to prescribe how localized texture bands and orientation clusters in tantalum are effectively resistant to sputter erosion. In this paper, results of atomic force microscopy (AFM) and orientation imaging microscopy (OIM{sup TM}) analyses on the eroded surface of a tantalum sputtering target are presented. Initial ...

2002-07-01

422

Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins

Short-hairpin RNA (shRNA)–mediated gene knockdown is a powerful tool for targeted gene silencing and an emerging novel therapeutic strategy. Recent publications, however, reported unexpected...Full Text Available

2009-09-01

423

Development of fission Mo-99 production technology

The Tetrahymena thermophila origin recognition complex (ORC) contains an integral RNA subunit, 26T RNA, which confers specificity to the amplified ribosomal DNA (rDNA) origin by base...Full Text Available

2009-02-04

424

Detection and Identification of Bartonella Species Pathogenic for Humans by PCR Amplification Targeting the Riboflavin Synthase Gene (ribC)

This R and D project is planed to supply domestic demands of Mo-99 through fission route, and consequently this project will be expected to rise up utilization of HANARO and KAERI's capability for marketing extension into domestic and oversea radiopharmaceutical market. HEU and LEU target types are decided and designed for fission Mo-99 production in domestic. Experimental study of target fabrication technology was performed and developed processing equipments. And conceptual design of target loading/unloading in/from HANARO device are performed. Tracer test of Mo-99 separation and purification process was performed, test results reach to Mo-99 recovery yield above 80% and decontamination factor above 1600. Combined Mo-99 separation and purification process was decided for hot test scheduled from next year, and performance test was performed. Conceptual design for modification of existing hot cell for fission Mo-99 ...

2001-05-01

425

Dengue virus protein recognition by virus-specific murine CD8+ cytotoxic T lymphocytes.

Several Bartonella species have now been implicated as human pathogens. The recovery of these fastidious organisms in the clinical microbiology laboratory remains difficult, and current...Full Text Available

2003-03-01

426

The identification of the protein targets for dengue virus-specific T lymphocytes may be useful for planning the development of subunit vaccines against dengue. We studied the recognition by murine...Full Text Available

1993-02-01

427

Deformation of CHS model

DNA base mismatch detection with bulky rhodium intercalators: synthesis and applications

We calculate mass spectrum of CHS model deformed by an exactly marginal operator, and find that there are tachyons which are not localized in the target space. Similar deformation is discussed in another CFT which corresponds to separated NS5-branes. A condensation of the tachyons is briefly argued.

2002-10-07

428

Cultural Variation in Vigilance and Precaution Themes.

The syntheses and applications of two metallointercalators, Rh(bpy)₂(chrysi)³⁺ and Rh(bpy)₂(phzi)³⁺, that target single base mismatches in DNA are...Full Text Available

2007-01-01

429

Comparative Analysis of State Fish Consumption Advisories Targeting Sensitive Populations

Determine the degree to which different precautionary themes are dominant in various target populations and how these themes are acquired. Assess cultural signals of potential danger: are there different kinds of potential danger in different cultures. Do...

2009-01-01

430

Characterization of the Decision Network for Wing Expansion in Drosophila Using Targeted Expression of the TRPM8 Channel

ObjectiveFish consumption advisories are issued to warn the public of possible toxicological threats from consuming certain fish species. Although developing fetuses and children...Full Text Available

2008-12-01

431

Characterization of Cytokinin and Adenine Transport in Arabidopsis Cell Cultures1[OA

After emergence, adult flies and other insects select a suitable perch and expand their wings. Wing expansion is governed by the hormone bursicon and can be delayed under adverse environmental...Full Text Available

2009-03-18

432

Carotenoid Crystal Formation in Arabidopsis and Carrot Roots Caused by Increased Phytoene Synthase Protein Levels

Cytokinins are distributed through the vascular system and trigger responses of target cells via receptor-mediated signal transduction. Perception and transduction of the signal can occur at the plasma...Full Text Available

2008-12-01

433

CDC45 and DPB11 are required for processive DNA replication and resistance to DNA topoisomerase I-mediated DNA damage

BackgroundAs the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches...Full Text Available

434

Beam-forming system for electrostatic accelerator

The antitumor agent camptothecin targets DNA topoisomerase I by reversibly stabilizing a covalent enzyme-DNA intermediate. The subsequent collision of DNA replication forks with these drug-enzyme-DNA...Full Text Available

1999-09-28

435

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions?

A simple beam-forming system is described for the electrostatic accelerator of the Scientific-Research Institute of Nuclear Physics of Moscow State University. The system allows the beam to be focused on targets at various distances for a conductor potential of from 1 to 4 MV and beam currents of up to 35 #mu# A.

1988-01-01

436

Analysis of target cell susceptibility as a basis for the development of a chemoprotective strategy against benzene-induced hematotoxicities.

This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found...Full Text Available

2009-03-01

437

Analysis of illegitimate genomic integration mediated by zinc-finger nucleases: implications for specificity of targeted gene correction

A goal of our research is to identify biochemical factors that underlie the susceptibility of bone marrow cell populations to benzene metabolites so as to develop a mechanistically based chemoprotective...Full Text Available

1996-12-01

438

An Eating Disorder Randomized Clinical Trial & Attrition: Profiles & Determinants of Dropout

BackgroundFormation of site specific genomic double strand breaks (DSBs), induced by the expression of a pair of engineered zinc-finger nucleases (ZFNs), dramatically increases the...Full Text Available

439

Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility: a case-control study

ObjectiveThis study sought to determine whether differential treatment effects in the targeted mechanisms of change and eating disorder (ED) symptoms are associated...Full Text Available

2011-05-01

440

Allosteric Drug Discrimination Is Coupled to Mechanochemical Changes in the Kinesin-5 Motor Core*

BackgroundMale infertility is a common cause of reproductive failure in humans. In mice, targeted deletions of the genes coding for FKBP6 or FKBP52, members of the FK506 binding...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Activated PPAR? Targets Surface and Intracellular Signals That Inhibit the Proliferation of Lung Carcinoma Cells

Essential in mitosis, the human Kinesin-5 protein is a target for >80 classes of allosteric compounds that bind to a surface-exposed site formed by the L5 loop. Not established is why there...Full Text Available

2010-06-11

442

ATG12 Conjugation to ATG3 Regulates Mitochondrial Homeostasis and Cell Death

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the nuclear hormone receptor superfamily. Their discovery in the 1990s provided insights...Full Text Available

2008-01-01

443

A pilot application of risk-based methods to establish in-service inspection priorities for nuclear components at Surry Unit 1 Nuclear Power Station

SUMMARYATG12, an ubiquitin-like modifier required for macroautophagy, has a single known conjugation target, another autophagy regulator called ATG5. Here, we identify ATG3 as...Full Text Available

2010-08-20

444

A duplex real-time RT-PCR assay for the detection of California serogroup and Cache Valley viruses

As part of the Nondestructive Evaluation Reliability Program sponsored by the US Nuclear Regulatory Commission, the Pacific Northwest Laboratory is developing a method that uses risk-based approaches to establish in-service inspection plans for nuclear power plant components. This method uses probabilistic risk assessment (PRA) results and Failure Modes and Effects Analysis (FEMA) techniques to identify and prioritize the most risk-important systems and components for inspection. The Surry Nuclear Power Station Unit 1 was selected for pilot applications of this method. The specific systems addressed in this report are the reactor pressure vessel, the reactor coolant, the low-pressure injection, and the auxiliary feedwater. The results provide a risk-based ranking of components within these systems and relate the target risk to target failure probability values for individual components. These results will be used to guide the development of ...

445

A Vasculature-Targeting Regimen of Pre-Operative Docetaxel with or without Bevacizumab for Locally Advanced Breast Cancer

A duplex TaqMan real-time RT-PCR assay was developed for the detection of California (CAL) serogroup viruses and Cache Valley virus (CVV), for use in human surveillance. The targets selected...Full Text Available

2009-10-01

446

A Master Conductor for Aggregate Clearance by Autophagy

PurposeTaxanes have effects on angiogenesis causing difficulties in separating biologic effects of chemotherapy from those due to angiogenesis inhibitors. This randomized...Full Text Available

2009-05-15

447

A Bragg curve counter with an internal production target for the measurement of the double-differential cross-section of fragment production induced by neutrons at energies of tens of MeV

Autophagic adapters including p62/SQSTM1 recognize polyubiquitinated autophagic targets such as toxic protein aggregates. Recently reporting in Molecular Cell, Filimonenko et al. provide evidence...Full Text Available

2010-05-18

448

Molybdaen-targets in the Research Centre J``ulich; Erzeugung von Molybdaen fuer die Medizin im FRJ-2 des Forschungszentrums Juelich

A Bragg curve counter equipped with an internal production target was developed for the measurements of double-differential cross-sections of fragment production induced by neutrons at energies of tens of MeV. The internal target permitted a large detection solid angle and thus the registration of processes at low production rates. In this specific geometry, the detection solid angle depends on the emission angle and the range of the particle. Therefore the energy, atomic number, and angle of trajectory of the particle have to be taken into account for the determination of the solid angle. For the selection of events with tracks confined within a defined cylindrical volume around the detector axis, a segmented anode was applied. The double-differential cross-sections for neutron-induced production of lithium, beryllium, and boron fragments from a carbon target were measured at 0 deg. for 65 MeV neutrons. The results are in ...

2009-11-11

449

The production of carrier-free lead-203

From autumn this year, the FRJ-2 of the Research Center Juelich will be supplying molybdenum targets to the Institut National des Radioelements in Fleurus, Belgium - which deals in medical radio-isotopes worldwide - thus helping to meet the need for technetium-99, which is used in the medical profession for diagnostic purposes because of its favourable radiological characteristics. Technetium-99 is formed as a result of the radioactive decay of molybdenum-99. For many years now, molybdenum has been produced by the irradiation of uranium in research reactors, so that the initiation of molybdenum production in the FRJ-2 is not especially new. What is unusual, however, are the particular peripheral conditions which result from the combination of the irradiation requirements, a predetermined target design and the technical characteristics of the reactor and which necessitated special solutions. This applies especially to the handling of the ...

1999-06-01

450

The CTOF measurements and Monte Carlo analyses of neutron spectra for backward direction from iron target irradiated by protons with energies from 400 to 1200 MeV

The chemical separation of carrier-free "2"0"3Pb following deuterium bombardment of a "2"0"3Tl target is described. Coprecipitation from strontium sulphate was used to separate the "2"0"3Pb from a solution of the thallium target in sulphuric acid. The SrSO_4 precipitate was filtered, washed and dissolved in hydrochloric acid, from which "2"0"3Pb was again separated by precipitation on sodium chloride from a strongly acid solution. Advantages of the system are discussed and optimum conditions given. (U.K.).

451

Target Diagnostic Instrument-Based Controls Framework for the National Ignition Facility

A calorimetric-time-of-flight (CTOF) technique was used for real-time, high-precision measurement of neutron spectrum at the angle of 175 degrees from the initial proton beam direction, which hits a face plane of a cylindrical iron target of 20 cm in diameter and 25 cm thick. A comparison was performed between the neutron spectra predicted by the MARS and the MCNPX code systems and measured by experiments for 0.4-, 0.6-, 0.8-, 1.0-, and 1.2-GeV protons.

2009-01-01

452

Simulation of High Power Deposition on Target Materials: Applications in Magnetic, Inertial Fusion, and High Power Plasma Lithography Devices

NIF target diagnostics are being developed to observe and measure the extreme physics of targets irradiated by the 192-beam laser. The response time of target materials can be on the order of 100ps--the time it takes light to travel 3 cm--temperatures more than 100 times hotter than the surface of the sun, and pressures that exceed 109 atmospheres. Optical and x-ray diagnostics were developed and fielded to observe and record the results of the first 4-beam experiments at NIF. Hard and soft x-ray spectra were measured, and time-integrated and gated x-ray images of hydrodynamics experiments were recorded. Optical diagnostics recorded backscatter from the target, and VISAR laser velocimetry measurements were taken of laser-shocked target surfaces. Additional diagnostics are being developed and commissioned to observe and diagnose ignition implosions, including various neutron and ...

2007-05-07

453

Real time neutron dosemeter response calculations

High power and particle deposition on target materials are encountered in many applications including magnetic and inertial fusion devices, nuclear and high energy physics applications, and laser and discharge produced plasma devices. Surface and structural damage to plasma-facing components due to the frequent loss of plasma confinement remains a serious problem for the Tokamak reactor concept. The deposited plasma energy causes significant surface erosion, possible structural failure, and frequent plasma contamination.

2006-01-01

454

Purification of cyclotron-produced {sup 203}Pb for labeling Herceptin

The response of a real time neutron dosemeter using a thin LiF target sandwiched between tow parallel surface barrier semiconductor detectors is studied for different neutron distributions and different angles of incidence. Calculations of the response function defined for a simultaneous detection by the two detectors of the particles emitted when the reaction "6Li(n,t)#alpha# occurs in the target are fulfilled by geometrical considerations of the reaction kinematics and the differential cross section variations. Finally, the efficiency of the studied detection systems is analyzed for dosimetric uses. (author).

1996-04-01

455

Proton beam monitoring and forming on the target of isotope complex at INR linac

A simple and rapid procedure was developed for the purification of cyclotron-produced {sup 203}Pb via the {sup 203}Tl(d,2n) {sup 203}Pb reaction. A Pb(II) selective ion-exchange resin, with commercial name Pb Resin from Eichrom Technologies, Inc., was used to purify {sup 203}Pb from the cyclotron-irradiated Tl target with excellent recovery of the enriched Tl target material. The purified {sup 203}Pb was used to radiolabel the monoclonal antibody Herceptin. The in vitro and in vivo properties of the {sup 203}Pb radioimmunoconjugate were evaluated.

2005-04-01

456

Production of carrier-free lead-203

Special beam extraction channel is used at INR linac for isotope production. It starts from 160 MeV measuring and matching area of accelerator. This choice of beam extraction enables to direct to the target of isotope complex a proton beam of wide energy range up to 160 MeV. It is important for production of different types of nuclides. The measuring results of proton beam behavior in this region are presented. Required beam forming in 160 MeV measuring and matching area of accelerator and in beam extraction channel is discussed.

2010-01-01

457

NuMI proton beam diagnostics and control: achieving 2 megawatt capability

The chemical separation of carrier-free /sup 203/Pb following deuterium bombardment of a /sup 203/Tl target is described. Coprecipitation from strontium sulphate was used to separate the /sup 203/Pb from a solution of the thallium target in sulphuric acid. The SrSO/sub 4/ precipitate was filtered, washed and dissolved in hydrochloric acid, from which /sup 203/Pb was again separated by precipitation on sodium chloride from a strongly acid solution. Advantages of the system are discussed and optimum conditions given.

1982-07-01

458

Neural solution to the target intercept problems in a gun fire control system

The NuMI proton beam at Fermilab currently delivers 120 GeV protons to the neutrino production target with design beam power capability to 400 kW. Upgrade capability to 700 kW is being prepared, with planning toward delivering 2.3 MW beam provided by the Project X accelerator upgrade plan. We report on the system of beam diagnostics and control used in operation of the NuMI beam. Also considered are the steps to provide a robust system for transport and targeting beam of 2 MW and beyond.

2008-10-01

459

British Library Electronic Table of Contents (United Kingdom)

Time delay neural networks trained with the backpropagation algorithm are derived for the gun fire control system to correct the miss distance between a target and the projectiles from the gun. Its performance is compared to optimum linear filter based on minimum mean square error [R.E. Kalman, A new approach to linear filtering and prediction problems, J. Basic Eng. 82D (1960) 35-44.]. The structure of the proposed neural controller is described and performance results are shown.

2007-01-01

460

Near-threshold production of {omega}-mesons in the pn{yields}d{omega} reaction

Modification of a gas jet transport system for the supression of heavy-mass fission product homologues

The first measurement of the pn{yields}d{omega} total cross-section has been achieved at mean excess energies Q{approx}28 and 57 MeV by using a deuterium cluster-jet target. The momentum of the fast deuteron was measured in the ANKE spectrometer at COSY-Juelich and that of the slow ''spectator'' proton (p{sub sp}) from the pd{yields}p{sub sp}d{omega} reaction in a silicon telescope placed close to the target. The cross-sections lie above those measured for pp{yields}pp{omega} but seem to be below theoretical predictions. (orig.)

2004-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Modification of a gas jet transport system for the supression of heavy-mass fission product homologues

The uranium target of a gas jet transport system for fission products has been modified by the addition of an aluminum cover foil. The new modification allows fission fragments of low mass to enter a continuous chemistry system while heavier fragments are stopped in the aluminum. The elimination of the heavier homologues allows development of simpler and faster chemical separation procedures. In addition, since the lighter fragments are slowed down by the cover foil a smaller target chamber can be used. This leads to a reduction in system holdup time.

1987-04-15

462

Mechanical variations of diffused plasma parameters in a double plasma device

The uranium target of a gas jet transport system for fission products has been modified by the addition of an aluminum cover foil. The new modification allows fission fragments of low mass to enter a continuous chemistry system while heavier fragments are stopped in the aluminum. The elimination of the heavier homologues allows development of simpler and faster chemical separation procedures. In addition, since the lighter fragments are slowed down by the cover foil a smaller target chamber can be used. This leads to a reduction in system holdup time. (orig.).

463

Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics

Experimentally it is shown that a movable grounded metallic plate placed inside a multi-dipole magnetic cage can vary the diffused plasma parameters such as density, plasma potential and electron temperature. Plasma is solely produced in the source section of a double plasma device by a dc hot filament discharge and a low-density plasma is produced in the target section by local ionization of neutral gas by the high energetic electrons coming from the source section. A grounded movable stainless steel plate is inserted in the target section of the device. The floating potential of the plate also changes depending on the position of the plate inside the magnetic cage.

2007-06-21

464

Inelastic electron--dipole-molecule scattering at sub-milli-electron-volt energies: HF and NH_3

Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. This conformational restriction results in unprecedented hybridization affinity towards complementary single stranded RNA and thus, makes LNA uniquely suited for mimicking RNA structures and sequence specific targeting of RNA in vitro or in vivo. The focus of this paper is on LNAantisense, LNA-modified siRNA (siLNA), and detection and analysis of microRNAs by LNA-modified oligonucleotide probes.

2005-01-01

465

Experimental research on X-ray spectrum emitted from hot laser-produced aluminium plasma

Studies of destruction of very-high-n (100targets HF and NH_3 are reported. Analysis of the data using the essentially-free-electron model suggests that, for ultralow electron energies (#approx#80 #mu#eV--1.4 meV), the cross section #sigma#(var-epsilon) for rotationally inelastic scattering of electrons by a polar target varies approximately as 1/var-epsilon, where var-epsilon is the electron energy.

466

Experimental Study of Plasma-Surface Interaction and Material Damage Relevant to ITER Type I Elms

The hot uniform aluminium plasma was produced by irradiating thin aluminium dotted foil smoothly with the 9th 0.53 ?m laser on Shenguang II laser facility. The emitted spectrum was measured from the front and tangential direction of the target with two crystal spectrometers, and the quantitative spectrum from the front of the target was obtained. The state of laser- produced plasma was simulated with the radiation hydrodynamics code MULTI-1D, and the emitted spectrum was calculated with the spectrum code of Collision-Radiation model under the simulated plasma state. The experimental spectrum accords with the simulated one. (authors)

2007-12-01

467

Electron beam therapy in carcinoma of the buccal mucosa

The paper presents experimental investigations of main features of plasma surface interaction and energy transfer to the material surface in dependence on plasma heat loads. The experiments were performed with QSPA repetitive plasma pulses of the duration of 0.25 ms and the energy density up to 2.5 MJ/m2. Surface morphology of the targets exposed to QSPA plasma screams is analyzed. Relative contribution of the Lorentz force and plasma pressure gradient to the resulting surface profile is discussed. development of cracking on the tungsten surface and swelling of the surface are found to be in strong dependence on initial temperature of the target.

2006-01-01

468

Electron beam therapy in carcinoma of the buccal mucosa

Electron beam therapy has often been used for radiation therapy of carcinomas of the buccal mucosa, though side effects, including stomatitis and dermatitis, are known to exist. We experimented using the additional back scatter and transit with the phantom under a lead plate within the irradiation field, in order to better focus the dose on the target volume moreover, we fabricated a mouthpiece fitted with a lead plate shaped to the target volume, and with respect to the influence of the mouthpiece on stomatitis and dermatitis, obtained good clinical results. (author).

1990-10-01

469

Direct dark matter identification with a hybrid detection technique

Electron beam therapy has often been used for radiation therapy of carcinomas of the buccal mucosa, though side effects, including stomatitis and dermatitis, are known to exist. We experimented using the additional back scatter and transit with the phantom under a lead plate within the irradiation field, in order to better focus the dose on the target volume moreover, we fabricated a mouthpiece fitted with a lead plate shaped to the target volume, and with respect to the influence of the mouthpiece on stomatitis and dermatitis, obtained good clinical results. (author).

470

Development of three-dimensional aiming point guidance law

In the quest to understand the ultimate nature of WIMPs, we propose the use of a hybrid detection technique: cylinders filled with liquefied noble gasses, acting as targets, are immersed inside a tank of Gd-doped ultra-pure water that provides an active and efficient veto against neutrons. The evaluation of the background rejection capabilities and physics potential of this instrument have been carried out through a full GEANT4 simulation, assuming the detector will be located at the Canfranc underground laboratory (in the Spanish Pyrenees). Our results compare very favourably with existing or planned experiments in the field. This technique is scalable and will allow to reach target masses of few tonnes in the next future.

2008-01-01

471

British Library Electronic Table of Contents (United Kingdom)

A new aiming point guidance (APG) law in 3-dimensional space is derived analytically. The guidance law is capable of guiding the missile (interceptor) to a straight collision course with respect to the target. The missile turning rate command proportional to the error angle between the current missile heading and the predicted aim point is calculated based on the predicted target position. Performance of the APG law is numerically evaluated and compared with the proportional navigation guidance (PNG) law. The result of numerical simulation shows that the APG law is superior to the PNG law in terms of miss distance, time of interception and final stage missile turning rate demand.

2010-01-01

472

Conformal radiation therapy: technical requirements and clinical applications

Spectroscopic investigation of the charge dynamics of heavy ions penetrating solid and gaseous targets

Conformal radiation therapy represents a considerable and attractive challenge in oncology. Its aim is mainly to improve local control by increasing the dose with an acceptable rate of complications. This work overviews the world literature on this subject. The technical and theoretical requirements are highlighted. These requirements include a precise definition of the target volume by digital imaging (essentially CT scan), but also clear view of the target volume and the organs at risk, a specific collimation of the beam, 3-D dose calculations, optimization procedures, and a rigid immobilization of the patient with verification of his position. Moreover, the clinical applications of conformal radiation therapy are reviewed and discussed. (authors). 80 refs., 1 tab.

1995-01-01

473

On the Utility of Antiprotons as Drivers for Inertial Confinement Fusion

This thesis presents the study of the slowing down process of fast heavy ions inside matter. In the framework of this research, the influence of the target density on the stopping process is investigated. Experiments on the interaction of {sup 48}Ca{sup 6+}-{sup 48}Ca{sup 10+} and {sup 26}Mg{sup 5+} ion beams with initial energies of 11.4 MeV/u and 5.9 MeV/u with solid and gaseous targets have been carried out. A novel diagnostic method, X-ray spectroscopy of K-shell projectile radiation, is used to determine the ion charge state in relation to its velocity during the penetration of fast heavy ions inside the stopping material. A spatially resolved analysis of the projectile and target radiation in solids is achieved for the first time. The application of low-density silica aerogels as stopping media provided a stretching of the ion stopping length by 20 - 100 times in comparison with solid quartz. The Doppler Effect ...

2007-01-15

474

Mapping a nucleolar targeting sequence of an RNA binding nucleolar protein, Nop25

By contrast to the large mass, complexity and recirculating power of conventional drivers for inertial confinement fusion (ICF), antiproton annihilation offers a specific energy of 90MJ/{micro}g and thus a unique form of energy packaging and delivery. In principle, antiproton drivers could provide a profound reduction in system mass for advanced space propulsion by ICF. We examine the physics underlying the use of antiprotons ({bar p}) to drive various classes of high-yield ICF targets by the methods of volumetric ignition, hotspot ignition and fast ignition. The useable fraction of annihilation deposition energy is determined for both {bar p}-driven ablative compression and {bar p}-driven fast ignition, in association with 0-D and 1-D target burn models. Thereby, we deduce scaling laws for the number of injected antiprotons required per capsule, together with timing and focal spot requirements. The kinetic energy of the injected antiproton ...

2003-10-20

475

Effects of ion-induced electron emission on magnetron plasma instabilities

Nop25 is a putative RNA binding nucleolar protein associated with rRNA transcription. The present study was undertaken to determine the mechanism of Nop25 localization in the nucleolus. Deletion experiments of Nop25 amino acid sequence showed Nop25 to contain a nuclear targeting sequence in the N-terminal and a nucleolar targeting sequence in the C-terminal. By expressing derivative peptides from the C-terminal as GFP-fusion proteins in the cells, a lysine and arginine residue-enriched peptide (KRKHPRRAQDSTKKPPSATRTSKTQRRRR) allowed a GFP-fusion protein to be transported and fully retained in the nucleolus. When the peptide was fused with cMyc epitope and expressed in the cells, a cMyc epitope was then detected in the nucleolus. Nop25 did not localize in the nucleolus by deletion of the peptide from Nop25. Furthermore, deletion of a subdomain (KRKHPRRAQ) in the peptide or amino acid substitution of lysine and arginine residues in the subdomain ...

2006-06-10

476

ELM resolved energy distribution studies in the JET MKII Gas-Box divertor using infra-red thermography

Some magnetron sputtering systems experience rapid oscillations in the current and voltage of the plasma discharge after several hours when equipped with certain targets. These oscillations often lead to the plasma becoming extinguished, a condition known as ''flame-out.'' This article details the study of two 90% W--10% Ti magnetron targets which differed in density. The higher density targets sometimes experienced flame-out after approximately 3 h of sputtering. The less dense material could be sputtered for the entire 15 h life of the target. Scanning electron microscopy pictures and atomic composition depth profiles were obtained using Auger electron spectroscopy. In addition, a Colutron-based ion source with a high vacuum system was used to measure ion-induced secondary electron emission coefficients as a function of energy, ion specie, and gas coverage. Analysis of the sample from the group that ...

477

Design of a closed 4 MW spallation target module with heat removal system for an ADS system

Using infra-red (IR) thermography, power loads onto the MKII Gas-Box divertor targets have been investigated in Type-I ELMy H-Mode plasmas at JET in medium current discharges (Ip = 2.6 MA and BT = 2.7 T). Heat fluxes are calculated from the measured divertor target tile surface temperatures taking into account the influence of co-deposited surface layers on tile surfaces. This is particularly important when estimating the energy deposition during transient events such as ELMs. Detailed energy balance analysis is used, both from IR and tile embedded thermocouples, to demonstrate an approximately constant ELM-averaged in/out divertor target asymmetry of ?0.55 and to show that the ELM in/out energy deposition ratio ranges from 1 : 1 to 2 : 1. The inter-ELM in/out ratio is close to the ELM-averaged value at low pedestal collisionalities and decreases down to values close to zero when the inner target plasma ...

2007-05-01

478

X-ray ablation measurements and modeling for ICF applications

At the Forschungszentrum Karlsruhe the HGF Strategy Fund project entitled 'Innovative Technology to Reduce Radiotoxicity' is performed which delivers an essential scientific contribution to the application oriented fundamental research for Accelerator Driven Systems (ADS). The addressed topics are in the areas of heavy liquid metal technology, thermalhydraulics and materials / corrosion. An ADS consists of three main components: an proton accelerator, a spallation target and a subcritical blanket. The strategic objective of an ADS is the transmutation of long-lived radioactive waste (plutonium, minor actinides and long-lived fission products) into short-lived isotopes or stable elements, thus closing the fuel cycle. The objective of one task of the HGF Strategy Fund Project is the numerical calculation of a closed spallation target module. In a consequent next step such a spallation target can be experimentally investigated ...

479

Target fabrication of low-density and nano porous materials to generate extreme ultraviolet (EUV)

X-ray ablation of material from the first wall and other components of an ICF (Inertial Confinement Fusion) chamber is a major threat to the laser final optics. Material condensing on these optics after a shot may cause damage with subsequent laser shots. To ensure the successful operation of the ICF facility, removal rates must be predicted accurately. The goal for this dissertation is to develop an experimentally validated x-ray response model, with particular application to the National Ignition Facility (NIF). Accurate knowledge of the x-ray and debris emissions from ICF targets is a critical first step in the process of predicting the performance of the target chamber system. A number of 1-D numerical simulations of NIF targets have been run to characterize target output in terms of energy, angular distribution, spectrum, and pulse shape. Scaling of output characteristics with variations of both ...

1996-09-01

480

Target fabrication of low-density and nano porous materials to generate extreme ultraviolet (EUV)

Our institute has been investigating laser-produced tin, lithium and xenon plasma for extreme ultraviolet (EUV) light source of EUV lithography system, and found the highest conversion efficiency of 3% at 13.5 nm in 2% bandwidth. In the present paper, we introduce fabrication methods of density-controlled tin targets to generate relatively monochromatic EUV, and higher conversion efficiency than bulk tin. The first method is the nano-template method, where liquid tin solution was immersed into a polymer film with monodispersed size nano-particle. The density can be controlled by tin concentration of the solution. The target can be classified into mass-limited target. We have shown a tendency of monochromatic EUV emission around 13.5 nm with decreasing of tin density. The intensity was higher than tin foil with bulk density. The tendency has a merit to mitigate heat effect of the first EUV mirror. The fabrication method has ...

2006-06-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Reactive magnetron sputtering of hard Si-B-C-N films with a high-temperature oxidation resistance

Our institute has been investigating laser-produced tin, lithium and xenon plasma for extreme ultraviolet (EUV) light source of EUV lithography system, and found the highest conversion efficiency of 3% at 13.5 nm in 2% bandwidth. In the present paper, we introduce fabrication methods of density-controlled tin targets to generate relatively monochromatic EUV, and higher conversion efficiency than bulk tin. The first method is the nano-template method, where liquid tin solution was immersed into a polymer film with monodispersed size nano-particle. The density can be controlled by tin concentration of the solution. The target can be classified into mass-limited target. We have shown a tendency of monochromatic EUV emission around 13.5 nm with decreasing of tin density. The intensity was higher than tin foil with bulk density. The tendency has a merit to mitigate heat effect of the first EUV mirror. The fabrication method has ...

2006-06-01

482

Pipe whip experiments involving impacts between pipes

Based on the results obtained for C-N and Si-C-N films, a systematic investigation of reactive magnetron sputtering of hard quaternary Si-B-C-N materials has been carried out. The Si-B-C-N films were deposited on p-type Si(100) substrates by dc magnetron co-sputtering using a single C-Si-B target (at a fixed 20% boron fraction in the target erosion area) in nitrogen-argon gas mixtures. Elemental compositions of the films, their surface bonding structure and mechanical properties, together with their oxidation resistance in air, were controlled by the Si fraction (5-75%) in the magnetron target erosion area, the Ar fraction (0-75%) in the gas mixture, the rf induced negative substrate bias voltage (from a floating potential to -500 V) and the substrate temperature (180-350 deg. C). The total pressure and the discharge current on the magnetron target were held constant at 0.5 Pa and 1 A, respectively. The ...

2005-11-01

483

Empirical Evaluation of a New Method for Calculating Signal to Noise Ratio (SNR) for Microarray Data Analysis

Dynamic pipe impact tests were performed in order to determine the impact conditions for which a 2 inch Schedule 80 carbon steel target pipe would not be broken if it were impacted during a pipe whip event created by a postulated break of an adjacent larger parallel pipe. Such pipe/pipe impact scenarios are of special interest for the feeder pipes of a CANDU reactor because the large number of closely spaced parallel feeder pipes that carry coolant between large primary system pipes and individual fuel channels in the reactor core makes it impractical to consider providing feeder pipe whip restraints. The testing which was performed involved simulating the behaviour of 3 inch and larger whipping pipes in order to study their impact with 2 inch target pipes pressurized at about 9 MPa with water at a temperature of about 290"0C. In a conservative simulation of the worst pipe/pipe impact event which it has been predicted could occur for adjacent ...

484

Co-product extraction studies on N-reactor PT-57 target materials

Signal-to-noise-ratio (SNR) thresholds for microarray data analysis were experimentally determined with an oligonucleotide array that contained perfect match (PM) and mismatch (MM) probes based upon four genes from Shewanella oneidensis MR-1. A new SNR calculation, called signal to both standard deviations ratio (SSDR) was developed, and evaluated along with other two methods, signal to standard deviation ratio (SSR), and signal to background ratio (SBR). At a low stringency, the thresholds of SSR, SBR, and SSDR were 2.5, 1.60 and 0.80 with oligonucleotide and PCR amplicon as target templates, and 2.0, 1.60 and 0.70 with genomic DNA as target templates. Slightly higher thresholds were obtained at the high stringency condition. The thresholds of SSR and SSDR decreased with an increase in the complexity of targets (e.g., target types), and the presence of background DNA, and a decrease in the composition ...

2008-03-06

485

Activation analysis of trace elements in titanium-molybdate target used for pre-formed TiMo gel based "9"9"mTc generator production and radionuclidic impurity of "9"9"mTc pertechnetate eluate

Single pellets (of approximately 70 g each) of irradiated lithium aluminate target from N-Reactor test PT-57 were used in a series of experiments to determine the extent to which the product tritium can be recovered by (a) vacuum outgassing of the target (thermal extraction-TX) and (b) in-vacuo chemical dissolution of the target in molten sodium tetraborate (flux extraction-FX). Five TX runs and seven FX runs were made. Thirty-five percent of the tritium was recovered in a form non-condensable at -196"0C. The remainder was recovered in a condensable form (as T_2O, HTO, etc.). Post-extraction analysis of the melt from the seven flux extractions showed that a maximum of 2 percent of the original amount of tritium remained and that target dissolution was essentially complete in 12 hours. Flux extraction of two pellets which had been subjected to thermal extraction showed less than 0.4 percent of the ...

486

Separation of "1"8"7","1"8"8Pt and "1"8"7","1"8"8Ir produced in "1"1B"4"+ irradiated tantalum target

A gel-type "9"9Mo-"9"9"mTc generator using Titanium-Molybdate target (TiMo-target) as column packing was developed in Radioisotope Department, the Dalat Nuclear Research Institute [1]. The concentration of trace elements in TiMo-target and radionuclidic purity in "9"9"mTc solution eluted from this type of generator were studied for quality control purpose. The monostandard method in neutron activation analysis has been applied to determine the concentration of trace impurities in titanium-molybdate target. For neutron activation analysis, TiMo samples were irradiated in the different channels of nuclear reactor IVV-9 at a neutron flux of maximum 2.1 x 10"1"3 n.cm"-"2s"-"1. The following chemical element impurities were determined: Na, W, Co, Fe, Zn, Ag, Sb and Cr. The gamma ray emitted nuclides found in "9"9"mTc solution eluted from our "9"9"mTc generator were the following: "6"5Zn, "6"0Co, "1"1"0"mAg, ...

2004-06-01

487

Safety and Environmental Aspects of Inertial Fusion Energy: An Overview of Recent Activities and Developments in the United States

Heavy ion irradiation on tantalum metal target with 57 MeV "1"1B"4"+ leads to the production of no-carrier-added radioisotopes of platinum, "1"8"7","1"8"8Pt and iridium, "1"8"7","1"8"8Ir in the matrix, which have been effectively separated from the bulk target and from each other with suitable anion exchangers employing liquid-liquid extraction (LLX). Also "1"8"8","1"8"9Pt and "1"8"8","1"8"9Ir radionuclides have been produced, from their short-lived precursors "1"8"8","1"8"9Au, in "1"2C"6"+ irradiated tantalum matrix. Gamma-spectroscopy has been utilised to determine the production, extent of separation and purity of the radiotracers at different stages of the experiment. (orig.)

488

Metallography of pitted aluminum-clad, depleted uranium fuel

During the past 2 yr, significant progress has been made in several areas related to the safety and environmental (S and E) aspects of inertial fusion energy (IFE). An updated methodology has been developed, and accident analyses have been performed for two IFE conceptual power plants and a target fabrication facility. Parallel to the consequence analyses of different accident scenarios, ongoing studies of accident initiating events are being used to support safety assessment and create a basic framework of types of events to consider in future risk characterization of new plant designs. Target designers/fabrication specialists have been provided with ranking information related to the S and E characteristics of candidate target materials. We have revisited waste management options for IFE, introducing the concept of clearance versus the traditional shallow land burial. A brief summary of results in each of these activities ...

2003-05-01

489

Measurement of electron energy fluence spectra from electron beam therapy machines

The storage of aluminum-clad fuel and target materials in the L-Disassembly Basin at the Savannah River Site for more than 5 years has resulted in extensive pitting corrosion of these materials. In many cases the pitting corrosion of the aluminum clad has penetrated in the uranium metal core, resulting in the release of plutonium, uranium, cesium-137, and other fission product activity to the basin water. In an effort to characterize the extent of corrosion of the Mark 31A target slugs, two unirradiated slug assemblies were removed from basin storage and sent to the Savannah River Technology Center for evaluation. This paper presents the results of the metallography and photographic documentation of this evaluation. The metallography confirmed that pitting depths varied, with the deepest pit found to be about 0.12 inches (3.05 nun). Less than 2% of the aluminum cladding was found to be breached resulting in less than 5% of the uranium surface ...

1994-12-01

490

Inclusive K{sup +}-meson production in proton-nucleus interactions

A technique capable of measuring the electron energy fluence spectra in a scattering medium was designed. These measurements were performed by setting a bremsstrahlung conversion target on the surface of a phantom, at an intermediate depth, and at a depth equal to electron mean range. The bremsstrahlung produced by the deceleration of electrons in the target was passed through an air channel in the phantom and passed forward by a pinhole collimator into a Na(Tl) detector. The measured pulse height data were unfolded to correct for the distortion of the spectrometer system by using the FORIST unfolding code. The unfolded bremsstrahlung spectra represent the electron energy fluence spectra convolution with the bremsstrahlung produced in the target. To generate the electron energy fluence spectra, the unfolded bremsstrahlung spectra were deconvoluted by using the MAZE2 unfolding code. CYLTRAN, a coupled electron-photon Monte ...

1984-01-01

491

Emission factors of polybrominated diphenyl ethers (PBDEs) from plastics processing and recycling facilities

The production of K{sup +}-mesons in pA(A=D,C,Cu,Ag,Au) collisions has been investigated at the COoler SYnchrotron COSY-Juelich for beam energies T{sub p}=1.0-2.3 GeV. Double differential inclusive pC cross-sections at forward angles {theta}{sub K+}<12 as well as the target mass dependence of the K{sup +} momentum spectra have been measured with the ANKE spectrometer. Far below the free NNthreshold at T{sub NN}=1.58 GeV the spectra reveal a high degree of collectivity in the target nucleus. From the target mass dependence of the cross-sections at higher energies, the repulsive in-medium potential of the K{sup +}-mesons can be deduced. Using pNcross-section parameterisations from the literature and our measured pD data we derive a cross-section ratio {sigma}(pn{yields}K{sup +}X)/{sigma}(pp{yields}K{sup +}X) {proportional_to}(3-4). (orig.)

2004-11-01

492

A heterogeneous dose distribution in simultaneous integrated boost: the role of the clonogenic cell density on the tumor control probability

With regard to polybrominated diphenyl ether (PBDE), there is few scientific knowledge on the emission patterns into the environment and exposure pathways to humans, and basic information is insufficient to consider what measures effective are. For the purpose of promoting risk reduction of target substances more effectively and efficiently, it is desirable to comprehend accurately the causal chain from the target substances utilization to the risk intake, and to evaluate the measures covering the whole applications of target substances. As the existing researches on the PBDE emission inventory, there are EU risk assessment report (European Chemical Bureau 2000, 2002, 2003), Danish EPA (1999), Palm et al.(2002) and Alcock et al. (2003). In addition, emissions of DecaBDE are published in TRI (Toxic Release Inventory) of US EPA. However, the primary information of the previous inventories is often the same and estimations ...

2004-09-15

493