targeted bisulfite sequencing: Topics by WorldWideScience.org

Isolation of Assimilatory- and Dissimilatory-Type Sulfite Reductases from Desulfovibrio vulgaris

PMID:11506019

2001-08-01

3

How DNA coiling enhances target localization by proteins

Bisulfite reductase (desulfoviridin) and an assimilatory sulfite reductase have been purified from extracts of Desulfovibrio vulgaris. The bisulfite reductase has absorption maxima...Full Text Available

1973-08-01

4

Targeted Capture and Next-Generation Sequencing Identifies C9orf75, Encoding Taperin, as the Mutated Gene in Nonsyndromic Deafness DFNB79

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

2008-10-14

5

The Chemical Reactions of Sulfur in the Citrate Process for Flue Gas Desulfurization.

Targeted genome capture combined with next-generation sequencing was used to analyze 2.9 Mb of the DFNB79 interval on chromosome 9q34.3, which includes 108 candidate genes. Genomic...Full Text Available

2010-03-12

6

Mapping a nucleolar targeting sequence of an RNA binding nucleolar protein, Nop25

The Bureau of Mines performed chemical research to elaborate details of sulfur chemistry pertaining to the citrate flue gas desulfurization process in which sulfur dioxide (SO2), absorbed (as bisulfite ion) in a buffered sodium citrate solution, is reduce...

1981-01-01

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Rapid, sensitive detection of Mycoplasma pneumoniae in simulated clinical specimens by DNA amplification.

Nop25 is a putative RNA binding nucleolar protein associated with rRNA transcription. The present study was undertaken to determine the mechanism of Nop25 localization in the nucleolus. Deletion experiments of Nop25 amino acid sequence showed Nop25 to contain a nuclear targeting sequence in the N-terminal and a nucleolar targeting sequence in the C-terminal. By expressing derivative peptides from the C-terminal as GFP-fusion proteins in the cells, a lysine and arginine residue-enriched peptide (KRKHPRRAQDSTKKPPSATRTSKTQRRRR) allowed a GFP-fusion protein to be transported and fully retained in the nucleolus. When the peptide was fused with cMyc epitope and expressed in the cells, a cMyc epitope was then detected in the nucleolus. Nop25 did not localize in the nucleolus by deletion of the peptide from Nop25. Furthermore, deletion of a subdomain (KRKHPRRAQ) in the peptide or amino acid ...

2006-06-10

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Molecular dynamics of a ?B DNA element: base flipping via cross-strand intercalative stacking in a microsecond-scale simulation

The polymerase chain reaction (PCR) was investigated as a means of diagnosing Mycoplasma pneumoniae infections. The target DNA sequence was a 375-bp segment of the P1 virulence protein. This DNA segment...Full Text Available

1992-12-01

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MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms

The sequence-dependent structural variability and conformational dynamics of DNA play pivotal roles in many biological milieus, such as in the site-specific binding of transcription factors to target...Full Text Available

2008-09-01

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Targeted integration of baboon endogenous virus in the BEVI locus on human chromosome 6.

MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences....Full Text Available

2003-08-19

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DNA rearrangements from #gamma#-irradiated normal human fibroblasts preferentially occur in transcribed regions of the genome

The infection of cultured human cells with baboon endogenous virus (BEV) frequently leads to an association of viral DNA with a specific genetic locus (termed BEVI, for baboon endogenous virus infection) on chromosome 6. Restriction endonuclease digestion of DNA from BEV-infected human cells and their derived somatic cell clones frequently revealed a common cellular DNA sequence in the proximity of one of the junctions between cellular DNA and the integrated virus. We propose that a short cellular DNA sequence, repeated on chromosome 6 and separated by unique DNA sequences, presents a high-affinity target for the integration of BEV in human cells. PMID:6401843

1983-01-13

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Alkylaldehyde-bisulfite adducts as cleavable surfactants

Full text: DNA rearrangement events leading to chromosomal aberrations are central to ionizing radiation-induced cell death. Although DNA double-strand breaks are probably the lesion that initiates formation of chromosomal aberrations, little is understood about the molecular mechanisms that generate and modulate DNA rearrangement. Examination of the sequences that flank sites of DNA rearrangement may provide information regarding the processes and enzymes involved in rearrangement events. Accordingly, we developed a method using inverse PCR that allows the detection and sequencing of putative radiation-induced DNA rearrangements in defined regions of the human genome. The method can detect single copies of a rearrangement event that has occurred in a particular region of the genome and, therefore, DNA rearrangement detection does not require survival and continued multiplication of the affected cell. Ionizing radiation-induced DNA ...

2003-08-17

13

British Library Electronic Table of Contents (United Kingdom)

Alkylaldehyde-bisulfite adducts with octyl, decyl, and dodecyl groups (CnS: n=8,10,12) were synthesized and the surface-active properties of the water-soluble compounds were compared. Exchange of the counter anions of C10S and C12S from sodium to tetramethylammonium solubilized them in water. The latter ammonium salt (C12S-QA) had surface activities as good as a classical surfactant, sodium dodecylsulfate, and dissociated promptly in weakly basic solution at room temperature to be a non-surfactant. It was also found that C12S-QA solubilized a hydrophobic substrate (solid), N,N-dimethyl-3-nitroaniline, in water and then separated it as a precipitate after alkali treatment.

2007-01-01

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Knowledge-Based Identification of the ERK2/STAT3 Signal Pathway as a Therapeutic Target for Type 2 Diabetes and Drug Discovery

British Library Electronic Table of Contents (United Kingdom)

Many existing agents for diabetes therapy are unable to restore or maintain normal glucose homeostasis or prevent the eventual emergence of hyperglycemia-related complication. Therefore, agents based on novel mechanisms are sought to complement and extend the current therapeutic approaches. Based on the initial paper research, we focused on active STAT3 as an attractive pharmacological target for type 2 diabetes. The subsequent text mining with a unique query to identify suppressors but not activators of STAT3 revealed the ERK2/STAT3 pathway as a novel diabetes target. The description of ERK2 inhibitors as diabetes target had not been found in our text mining research at present. The mechanism-based peptide inhibitor for ERK2 was identified using the knowledge of the KIM sequence, which ha...

2011-01-01

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Exploiting rice-sorghum synteny for targeted development of EST-SSRs to enrich the sorghum genetic linkage map.

Nuclear Targeting of Methyl-Recycling Enzymes in Arabidopsis thaliana Is Mediated by Specific Protein Interactions.

The sequencing and detailed comparative functional analysis of genomes of a number of select botanical models open new doors into comparative genomics among the angiosperms, with potential benefits for improvement of many orphan crops that feed large populations. In this study, a set of simple sequence repeat (SSR) markers was developed by mining the expressed sequence tag (EST) database of sorghum. Among the SSR-containing sequences, only those sharing considerable homology with rice genomic sequences across the lengths of the 12 rice chromosomes were selected. Thus, 600 SSR-containing sorghum EST sequences (50 homologous sequences on each of the 12 rice chromosomes) were selected, with the intention of providing coverage for corresponding homologous regions of the sorghum genome. Primer pairs were designed and polymorphism detection ...

2009-08-08

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Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics

Numerous transmethylation reactions are required for normal plant growth and development. S-adenosylhomocysteine hydrolase (SAHH) and adenosine kinase (ADK) act coordinately to recycle the by-product of these reactions, S-adenosylhomocysteine (SAH) that would otherwise competitively inhibit methyltransferase (MT) activities. Here, we report on investigations to understand how the SAH produced in the nucleus is metabolized by SAHH and ADK. Localization analyses using green fluorescent fusion proteins demonstrated that both enzymes are capable of localizing to the cytoplasm and the nucleus, although no obvious nuclear localization signal was found in their sequences. Deletion analysis revealed that a 41-amino-acid segment of SAHH (Gly(150)-Lys(190)) is required for nuclear targeting of this enzyme. This segment is surface exposed, shows unique sequence conservation patterns in plant SAHHs, and possesses additional features of ...

2011-10-01

17

DEFF Research Database (Denmark)

Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. This conformational restriction results in unprecedented hybridization affinity towards complementary single stranded RNA and thus, makes LNA uniquely suited for mimicking RNA structures and sequence specific targeting of RNA in vitro or in vivo. The focus of this paper is on LNAantisense, LNA-modified siRNA (siLNA), and detection and analysis of microRNAs by LNA-modified oligonucleotide probes.

2005-01-01

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Removal of noxious contaminants from gas

Study on possible fuel layering sequence for FIREX target

Gas contaminated with at least one noxious component, such as SO/sub 2/, HF or NOx, is scrubbed with a clear, calcium-containing, initially-basic washing liquid which also comprises the anion of a strong inorganic acid, that of a polybasic carboxylic acid and, optionally, that of a monobasic carboxylic acid. Apparatus is provided for scrubbing the gas, oxidizing produced calcium bisulfite to calcium sulfate, separating out and removing solids, and recirculating clarified liquid for scrubbing contaminated gas.

1980-09-16

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Amino acid sequences that determine the nuclear localization of yeast histone 2B.

A new procedure of fuel layering for the Fast Ignition Realization Experiment (FIREX) target is proposed. A conical laser guide heating technique was experimentally demonstrated in principle as the followings. It employed the target consisting of a polystyrene (PS) shell, a fill tube and a conical laser guide. At first, liquid fuel was fed into the shell and existed around the conical laser guide because the surface tension of the fuel must cause it. Then, it was solidified. The laser light provided a heat source to the conical laser guide so that the solid fuel was moved to the other interior of the shell. This process resulted in missing solid fuel around the conical laser guide. To fill the vacant space, liquid fuel was added as temperature was raised to the melting point. After the liquid fuel addition, temperature was lowered to the solidification point again. During this process, most of the solid fuel could survive.

2010-08-01

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The Sorcerer II Global Ocean Sampling Expedition: Expanding theUniverse of Protein Families

Histone-beta-galactosidase protein fusions were used to identify the domain of yeast histone 2B, which targets this protein to the nucleus. Amino acids 28 to 33 in H2B were required for nuclear localization...Full Text Available

1987-11-01

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Continuous intensity map optimization (CIMO): A novel approach to leaf sequencing in step and shoot IMRT

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

2006-03-23

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Closing the loop in cortically-coupled computer vision: a brain-computer interface for searching image databases.

A new leaf-sequencing approach has been developed that is designed to reduce the number of required beam segments for step-and-shoot intensity modulated radiation therapy (IMRT). This approach to leaf sequencing is called continuous-intensity-map-optimization (CIMO). Using a simulated annealing algorithm, CIMO seeks to minimize differences between the optimized and sequenced intensity maps. Two distinguishing features of the CIMO algorithm are (1) CIMO does not require that each optimized intensity map be clustered into discrete levels and (2) CIMO is not rule-based but rather simultaneously optimizes both the aperture shapes and weights. To test the CIMO algorithm, ten IMRT patient cases were selected (four head-and-neck, two pancreas, two prostate, one brain, and one pelvis). For each case, the optimized intensity maps were extracted from the Pinnacle"3 treatment planning system. The CIMO algorithm was applied, and the ...

2006-04-01

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Evolution of Hox Post-Transcriptional Regulation by Alternative Polyadenylation and MicroRNA Modulation Within 12 Drosophila Genomes.

We describe a closed-loop brain-computer interface that re-ranks an image database by iterating between user generated 'interest' scores and computer vision generated visual similarity measures. The interest scores are based on decoding the electroencephalographic (EEG) correlates of target detection, attentional shifts and self-monitoring processes, which result from the user paying attention to target images interspersed in rapid serial visual presentation (RSVP) sequences. The highest scored images are passed to a semi-supervised computer vision system that reorganizes the image database accordingly, using a graph-based representation that captures visual similarity between images. The system can either query the user for more information, by adaptively resampling the database to create additional RSVP sequences, or it can converge to a 'done' state. The done state includes a final ranking of the ...

2011-05-12

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Video object segmentation using color-component-selectable learning for self-organizing maps

Hox genes encode a family of transcriptional regulators that operate differential developmental programs along the anteroposterior axis of bilateral animals. Regulatory changes affecting Hox gene expression are believed to have been crucial for the evolution of animal body plans. In Drosophila melanogaster, Hox expression is post-transcriptionally regulated by microRNAs (miRNAs) acting on target sites located in the 3' untranslated regions (3'UTRs) of Hox mRNAs. Notably, recent work has shown that during D. melanogaster development Hox genes produce mRNAs with variable 3'UTRs (short and long forms) in different sets of tissues as a result of alternative polyadenylation; importantly, Hox short and long 3'UTRs contain very different target sites for miRNAs. Here, we use a computational approach to explore the evolution of Hox 3'UTRs treated with especial regard to miRNA regulation. Our work is focused on the 12 Drosophila species for which ...

2011-03-24

25

British Library Electronic Table of Contents (United Kingdom)

In this article, self-organizing-map-based video object segmentation is proposed, assuming that either Y-quantification or HSV-quantification can be systematically selected. Given a video sequence, the value of the probability density function for each component value is calculated according to a kernel estimation at the first frame. Some areas randomly chosen from the background are then examined, using each component value, to judge whether or not they include the target object. The quantification is determined so that the frequency of occurrence of false extractions can be reduced. The data presented to the maps are generated based on the selected quantification. Experimental results show that the proposed method recognizes the target object well.

2011-01-01

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Hsp90 gene, an additional target for discrimination between the potato cyst nematodes, Globodera rostochiensis and G. pallida, and the related species, G. tabacum tabacum

British Library Electronic Table of Contents (United Kingdom)

The heat-shock gene, Hsp90, was targeted as a new variable genomic region to supplement other DNA-based tests for identification and discrimination of Globodera pallida, G. rostochiensis and G. tabacum tabacum. Populations of the potato cyst nematodes, G. pallida and G. rostochiensis (PCN), originating from Canada, France, Belgium and USA, together with two populations of G. tabacum tabacum from the USA and France were used for the amplification of a fragment of the Hsp90 gene. General and specific primers and probes for each species were derived from the consensus and non-consensus regions of the aligned sequences, respectively. A triplex conventional PCR assay, using a general forward and reverse or three specific reverse primers, as well as a real-time PCR using general primers and spec...

2011-01-01

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Analysis of forward mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in the bacteriophage P22 mnt repressor gene

Transcriptional regulation in Drosophila: the post-genome challenge.

We describe the isolation and genetic characterization of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutations in the phage P22 mnt repressor gene cloned in plasmid pBR322. Mutations in the mnt repressor gene or its operator on this plasmid, pPY98, confer a tetracycline resistance phenotype, whereas the wild-type plasmid confers tetracycline sensitivity. Cells carrying pPY98 were briefly exposed to MNNG to give 20 to 40% survival and a 50- to 100-fold increase in tetracycline-resistant cells. DNA sequence analysis showed that 29 to 30 MNNG-induced mutations were GC-to-AT transitions and one was an AT-to-GC transition. About 80% of the mutations are in three hotspots. This mutation spectrum is consistent with the proposed mechanism of mutagenic action of MNNG, which involves mispairing of an alkylated base, O/sup 6/-methylguanine. The mnt gene may be a useful target for determining mutagenic specificity at the nucleotide level ...

1986-04-01

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Vibrational and Rotational Sequences in "1"0"1Mo And "1"0"3","4Ru Studied via Multinucleon Transfer Reactions

Drosophila melanogaster has long been at the forefront of studies of transcriptional regulation in animals. Many fundamental ideas--such as cis control elements that act over long distances, the regulation of development by hierarchical cascades of transcription factors, dosage compensation, and position effect variegation--originated from studies of the fruit fly. The recent completion of the euchromatic DNA sequence of Drosophila is another breakthrough. The sequence data highlight important unanswered questions. For example, only one-fifth of the 124 Mb of Drosophila euchromatic DNA codes for protein. The function of the remaining 100 Mb of mostly unique DNA is largely unknown. Some proportion of this non-reading frame DNA must encode the functional recognition sites targeted by the approximately 700 sequence-specific DNA binding proteins that regulate transcription in Drosophila, but what ...

2001-03-01

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Mapping cis-Regulatory Domains in the Human Genome UsingMulti-Species Conservation of Synteny

The near-yrast states of "1"0"1_4_2Mo_5_9 and "1"0"3","4_4_4 Ru_5_9_,_6_0 have been studied following their population via heavy-ion multinucleon transfer reactions between a "1"3"6Xe beam and a thin, self-supporting "1"0"0Mo target. The ground state sequence in "1"0"4Ru can be understood as demonstrating a simple evolution from a quasi-vibrational structure at lower spins to statically deformed, quasi-rotational excitation involving the population of a pair of low-#OMEGA# h_1_1_/_2 neutron orbitals. The effect of the decoupled h_1_1_/_2 orbital on this vibration-to-rotational evolution is demonstrated by an extension of the ''E-GOS'' prescription to include odd-A nuclei. The experimental results are also compared with self-consistent Total Routhian Surface calculations which also highlight the polarising role of the highly aligned neutron h_1_1_/_2 orbital in these nuclei. (author)

2005-04-01

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Oxidation of Elemental Sulfur by Fusarium solani Strain THIF01 Harboring Endobacterium Bradyrhizobium sp.

Our inability to associate distant regulatory elements with the genes that they regulate has largely precluded their examination for sequence alterations contributing to human disease. One major obstacle is the large genomic space surrounding targeted genes in which such elements could potentially reside. In order to delineate gene regulatory boundaries we used whole-genome human-mouse-chicken (HMC) and human-mouse-frog (HMF) multiple alignments to compile conserved blocks of synteny (CBS), under the hypothesis that these blocks have been kept intact throughout evolution at least in part by the requirement of regulatory elements to stay linked to the genes that they regulate. A total of 2,116 and 1,942 CBS>200 kb were assembled for HMC and HMF respectively, encompassing 1.53 and 0.86 Gb of human sequence. To support the existence of complex long-range regulatory domains within these CBS we analyzed the prevalence and ...

2005-06-13

31

British Library Electronic Table of Contents (United Kingdom)

Nineteen fungal strains having an ability to oxidize elemental sulfur in mineral salts medium were isolated from deteriorated sandstones of Angkor monuments. These fungi formed clearing zone on agar medium supplemented with powder sulfur due to the dissolution of sulfur. Representative of the isolates, strain THIF01, was identified as Fusarium solani on the basis of morphological characteristics and phylogenetic analyses. PCR amplification targeting 16S rRNA gene and analyses of full 16S rRNA gene sequence indicated strain THIF01 harbors an endobacterium Bradyrhizobium sp.; however, involvement of the bacterium in the sulfur oxidation is still unclear. Strain THIF01 oxidized elemental sulfur to thiosulfate and then sulfate. Germination of the spores of strain THIF01 was observed in a liqui...

2010-01-01

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Designing engineering upgradability into magnetic resonance imagers: Impact on future costs

Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola

MRI is a powerful diagnostic modality of expanding availability. Equipment and installation amount to nearly $2M per site. An important component of diagnostic efficacy is not just equipment costs but also replacement costs, which for x-ray CT amount to 14-20% of the purchase cost per year; and in the early years of that technology reached 30-50%. The authors show how design choices made during the RandD stages of MRI development have allowed them to improve system performance parameters such as data reconstruction, archiving and display speeds, computational capabilities, operator interfaces, imaging sequence flexibility and signal-to-noise levels. Over the last four years these improvements have been made at a retrofit cost well below the target of 6-7% of the purchase price per year.

1987-02-01

33

British Library Electronic Table of Contents (United Kingdom)

Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzy...

2006-01-01

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Automated motion estimation of root responses to sucrose in two Arabidopsis thaliana genotypes using confocal microscopy

British Library Electronic Table of Contents (United Kingdom)

Root growth is a highly dynamic process influenced by genetic background and environment. This paper reports the development of R scripts that enable root growth kinematic analysis that complements a new motion analysis tool: PlantVis. Root growth of Arabidopsis thaliana expressing a plasma membrane targeted GFP (C24 and Columbia 35S:LTI6b-EGFP) was imaged using time-lapse confocal laser scanning microscopy. Displacement of individual pixels in the time-lapse sequences was estimated automatically by PlantVis, producing dense motion vector fields. R scripts were developed to extract kinematic growth parameters and report displacement to ?0.1 pixel. In contrast to other currently available tools, Plantvis-R delivered root velocity profiles without interpolation or averaging across the root s...

2011-01-01

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Plasma membrane as the target site of cholic acid analogs.

Electrochemical detection of DNA hybridization based on silver-enhanced gold nanoparticle label

Although the mechanism is unknown, Calculus Bovis and its active components, cholic acid analogs (CAAs), have been used in China to treat a wide range of diseases. Based on the previous finding that the potency of CAA is strongly dependent on the intrinsic surface activity, this paper aimed to investigate the role of the plasma membrane in the pharmacological activity of CAAs. First, CAAs (0.1 mM) caused a surface activity-dependent depression on ATPase activity in the cell membrane extract, but it had no effects on other cellular extracts, suggesting an indispensable role of the membrane environment for pharmacological activity. Second, CAAs lowered the membrane fluidity of cultured Caco-2 cells with the same rank-order of potency sequence. Third, the hypothesis that any functional protein located on the membrane is influenced by changes in cellular membrane fluidity was supported by: ileal contraction that was induced by acetylcholine and mediated by the ...

2011-08-03

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Bifurcation and complex dynamics of a two-prey two-predator system concerning periodic biological and chemical control

An electrochemical detection method for analyzing sequence-specific DNA using gold nanoparticle DNA probes and subsequent signal amplification step by silver enhancement is described. The assay relies on the electrostatic adsorption of target oligonucleotides onto the sensing surface of the glassy carbon electrode (GCE) and its hybridization to the gold nanoparticle-labeled oligonucleotides DNA probe. After silver deposition onto gold nanoparticles, binding events between probe and target were monitored by the differential pulse voltammetry (DPV) signal of the large number of silver atoms anchored on the hybrids at the electrode surface. The signal intensity difference permits to distinguish between the match of two perfectly matched DNA strands and the near-perfect match where just one base pair was wrong. Coupled with this 'nanoparticle-promoted' reduction of silver signal amplification method, the ...

2002-10-03

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Comparison of contrast-enhanced flash and turbo-flash with conventional spin-echo sequences as a basis for morphologocal MR imaging for planning high-precision radiotherapy of intracranial brain tumors. Comparison of FLASH-, turbo-FLASH and SE-sequences; Morphologische MR-Bildgebung mit T1-gewichteten Sequenzen zur radiotherapeutischen Zielvolumendefinition von intrakraniellen Tumoren. Vergleich von FLASH-, Turbo-FLASH- und SE-Sequenzen

In this paper, we investigate the dynamic behaviors of a two-prey two-predator system with impulsive effect concerning biological and chemical control strategy-periodic releasing natural enemies and spraying pesticide at different fixed time. By applying the Floquet theory of linear periodic impulsive equation and small amplitude perturbation method, we prove that there exists a globally asymptotically stable two-prey-eradication periodic solution when the impulsive period is less than some critical value. The conditions for the permanence of the system are given, and meanwhile the conditions for the extinction of one of the two prey species and permanence of the remaining three species are given. Our results suggest a new approach in pest control. The target pest population can be driven to extinction and the non-target pest can be permanent by choosing impulsive period. With the increasing of the predation rate for the super competitor and ...

2008-07-15

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Interactive computer programs in sequence data analysis.

Introduction: The goal of this study was to compare contrast-enhanced T1-weighted Flash and Turbo-Flash sequences with conventional spin-echo sequences as a basis for planning high-precision radiotherapy. Methods: A total of 25 consecutive patients with different intracranial tumors and a disrupted blood-brain barrier were studied. T1-weighted Flash, Turbo-Flash and conventional spin-echo images were evaluated after controlled 30-s infusion of 0.1 mmol/kg body weight of Gd-DTPA. The evaluation of the three sequences included the measurement of the signal- and contrast-to-noise ratios, the visual inspection of the tumors and artifacts, and the measurement of tumor size. Results: The signal- and contrast-to-noise ratios were significantly (P<0.05-0.01) lower for Flash and Turbo-Flash than for conventional spin-echo sequences. However, visual inspection of the contrast-enhancing tumors revealed in 23 ...

1997-03-01

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Evaluation of methods to measure surface level in waste storage tanks: Second test sequence

We present interactive computer programs for the analysis of nucleic acid sequences. In order to handle these programs, minimum computer experience is sufficient. The nucleotide sequence of the human...Full Text Available

1982-01-11

40

Targeting vaccines to dendritic cells

This report describes the results of a program conducted at the Pacific Northwest Laboratory (PNL) and Westinghouse Hanford Company (WHC) to identify alternative methods to measure the surface level in the waste tanks. This program examined commercially available devices for measuring the distance to a target. This is a continuation of a program started in FY93. In the first test sequence, tests were performed.on five devices to determine their applicability to measure the surface level in the waste tanks. The devices were the Enraf-Nonius{trademark} Model 872 Radar Gauge, the Enraf-Nonius{trademark} Model 854 Advanced Technology Gauge (ATG), the Stanley Tool Laser Measuring Device, the Robertshaw Inven-Tel{reg_sign} Precision Level Gauge, and the Micro Switch Model 942 Acoustic Sensor. In addition, discussions were held with several manufacturer representatives regarding other potential devices. The results of these tests were documented in a ...

1993-09-01

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DEFF Research Database (Denmark)

Udgivelsesdato: 2002-Mar

2002-01-01

42

Constraints on target chamber first wall and target designs that will enable NIF debris shields to survive

EDI as a Treatment Module in Recycling Spent Rinse Waters

Constraints on target chamber first wall and target designs that will enable NIF debris shields to survive

1999-01-01

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Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains?

Recycling of the spent rinse water discharged from the wet benches commonly used in semiconductor processing is one tactic for responding to the targets for water usage published in the 1997 National Technology Roadmap for Semiconductors (NTRS). Not only does the NTRS list a target that dramatically reduces total water usage/unit area of silicon manufactured by the industry in the future but for the years 2003 and beyond, the NTRS actually touts goals which would have semiconductor manufacturers drawing less water from a regional water supply per unit area of silicon manufactured than the quantity of ultrapure water (UPW) used in the production of that same silicon. Achieving this latter NTRS target strongly implies more widespread recycling of spent rinse waters at semiconductor manufacturing sites. In spite of the fact that, by most metrics, spent rinse waters are of much higher purity than incoming municipal waters, ...

1999-08-11

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A shockproof container for the transport and storage of fragile or reactive target foils

Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium...Full Text Available

2011-02-01

45

Post-column reaction detector for platinum(II) antieoplastic agents

A shockproof container for the transport and storage of fragile or reactive target foils

1971-01-01

46

Economic evaluation of an electrolytic process to recover lead from scrap batteries. Information circular/1986

The development and evaluation of a post-column reaction detector sensitive to platinum(II) complexes is presented in which sodium bisulfite is used as the derivatizing agent with potassium dichromate as an activating agent. The influences of mobile phase changes (i.e., pH, organic modifiers, electrolytes), oxygen, metal ions, and order of reagent addition on reaction kinetics and product yield are defined and used in optimization of detector response. Detection at lambda/sub max/ 290 nm results in an on-line post-column sensitivity of 40-60 ng/mL for selected cis-dichloroplatinum complexes and a sensitivity of 300-1200 ng/mL for four (substituted)-malonato-platinum complexes. The reaction detector is used to monitor the kinetics of aquation of cisplatin (CDDP) and to quantitate CDDP degradation in plasma. As the sensitivity for CDDP in plasma is comparable to that achieved from high pressure liquid chromatography (HPLC) effluent fractionation/off-line flameless ...

1984-03-01

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Molecular studies of the uncoupling protein

The publication is an economic evaluation of the Bureau of Mines electrolytic process for recovering lead from scrap lead-acid batteries. In this process, scrap batteries are crushed and separated into metal and sludge fractions. The metal fraction is cast as anodes and electro-refined. Lead in the sludge fraction is converted to lead carbonate by reaction with an ammonium carbonate-ammonium bisulfite solution. The lead carbonate is then dissolved in a fluosilicic acid electrolyte from which pure lead metal is electrowon. A cost estimate is presented for a plant capable of processing 10,000 scrap batteries per day. The fixed capital cost for the plant is estimated to be $14 million on a fourth quarter 1984 basis. Operating costs are estimated to be $0.15/lb Pb recovered. Assuming a lead selling price of $0.17/lb, the interest rate of return on investment after taxes is 11 pct. A lead selling price of about $0.21/lb is needed to obtain a 20-pct-interest rate of ...

1986-01-01

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Expression profiles of precursor and mature microRNAs under dehydration and high salinity shock in Populus euphratica

The uncoupling protein (UCP) is a proton/anion transporter found in the inner mitochondrial membrane of brown adipocyte. Although UCP has nor been detected in mitochondria from any other tissue, it shares structural and catalytic properties with several other mitochondrial carrier proteins. Although UCP was discovered only recently it is one of the most extensively studied mitochondrial carrier proteins.More recently, the mouse, rat, and human genes encoding for UCP have been isolated and sequenced. The availability of these various tools has led to several significant observations. UCP gene expression is strongly controlled at the level of transcription by signals that are activated after the stimulation of brown adipocytes by norepinephrine. The comparison of UCP gene with the genes encoding the adenine nucleotide translocator revealed the existence of structural and evolutionary homologies. Moreover, in humans the UCP gene and one form of adenine nucleotide ...

1991-06-01

49

British Library Electronic Table of Contents (United Kingdom)

MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11 families and 13 mature miRNAs from 12 families by PCR amplification in P. euphratica. To detect expression differences in mature miRNAs and their precursors under dehydration and high salinity shock in P. euphratica, we examined 14 miRNA precursors from 13 miRNA families and 17 mature miRNAs from 17 miRNA families using the SYBR Green RT?PCR assay. This is the first report of expression profiles for both precursor and mature miRNAs in P. euphratica. By profiling both the matu...

2011-01-01

50

Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function.

Containment of genetically engineered organisms after application to subsurface environments. Technical completion report

The GTPases comprise a protein superfamily of highly conserved molecular switches adapted to many diverse functions. These proteins are found in all domains of life and often perform essential roles in fundamental cellular processes. Analysis of data from genome sequencing projects demonstrates that bacteria possess a core of 11 universally conserved GTPases (elongation factor G and Tu, initiation factor 2, LepA, Era, Obg, ThdF/TrmE, Ffh, FtsY, EngA and YchF). Investigations aimed at understanding the function of GTPases indicate that a second conserved feature of these proteins is that they elicit their function through interaction with RNA and/or ribosomes. An emerging concept suggests that the 11 universal GTPases are either necessary for ribosome function or transmitting information from the ribosome to downstream targets for the purpose of generating specific cellular responses. Furthermore, it is suggested that progenitor GTPases were ...

2001-07-01

51

Cholinergic systems in brain development and disruption by neurotoxicants: nicotine, environmental tobacco smoke, organophosphates

The feasibility of containing genetically engineered bacteria with enhanced dehalogenating properties for in situ bioremediation was investigated. (1) An agarose matrix microbead protocol and a detection system for contained microorganisms or DNA were developed. Multiplex Polymerase Chain Reaction (PCR) allowed tracking of a consortium of encapsulated organisms or several gene targets from a single species. Gene sequences encoding the enzymes responsible for initiating the biodegradation of toluene, octane, and 2,4-D were detected by multiplex PCR and nucleic acid probes from similar to 1-10 biodegradative cells/g soil. Improved DNA extraction methods resulted in PCR reactions detecting similar to 6 cells/g soil. (2) The pcpB gene (for the broad-spectrum detoxicant pentachlorophenol (PCP) hydroxylase) isolated from Flavobacterium sp. strain ATCC 39723 was used in attempts to develop an improved dehalogenating recombinant microorganism for ...

1993-06-01

52

Recognition of subaerial exposure and flooding surfaces in carbonate-siliciclastic eolianites and marine carbonate sequences in southwester Kansas

Acetylcholine and other neurotransmitters play unique trophic roles in brain development. Accordingly, drugs and environmental toxicants that promote or interfere with neurotransmitter function evoke neurodevelopmental abnormalities by disrupting the timing or intensity of neurotrophic actions. The current review discusses three exposure scenarios involving acetylcholine systems: nicotine from maternal smoking during pregnancy, exposure to environmental tobacco smoke (ETS), and exposure to the organophosphate insecticide, chlorpyrifos (CPF). All three have long-term, adverse effects on specific processes involved in brain cell replication and differentiation, synaptic development and function, and ultimately behavioral performance. Many of these effects can be traced to the sequence of cellular events surrounding the trophic role of acetylcholine acting on its specific cellular receptors and associated signaling cascades. However, for chlorpyrifos, additional ...

2004-07-15

53

Development of a geometrically accurate imaging protocol at 3 Tesla MRI for stereotactic radiosurgery treatment planning

Recent work on the St. Louis Limestone in southwestern Kansas has demonstrated that these units contain a significant eolian facies component (up to 80-90% of total unit thickness). Reservoir intervals within the St. Louis are confined to relatively thin subtidal grainstones that, in turn, are capped by a muddy carbonate and shale facies. Critical to exploration and development of these grain-shoal reservoirs is an understanding of their spatial and stratigraphic distribution. Core through the St. Louis and St. Genevieve limestones has been examined and features have been recognized at the top of the eolianites. These surfaces are interpreted as long-term exposure surfaces. The contact between the subtidal grainstone shoals and the overlying muddy carbonate and shale facies is relatively sharp and is interpreted as representing a flooding surface separating shoal from muddy-open shelf facies. In the St. Louis Limestone, the subtidal carbonate grainstone reservoir intervals consist of ...

1993-09-01

54

Recycling heterogeneous americium targets in a boiling water reactor

The purpose of this study is to develop a geometrically accurate imaging protocol at 3 T magnetic resonance imaging (MRI) for stereotactic radiosurgery (SRS) treatment planning. In order to achieve this purpose, a methodology is developed to investigate the geometric accuracy and stability of 3 T MRI for SRS in phantom and patient evaluations. Forty patients were enrolled on a prospective clinical trial. After frame placement prior to SRS, each patient underwent 3 T MRI after 1.5 T MRI and CT. MR imaging protocols included a T1-weighted gradient echo sequence and a T2-weighted spin echo sequence. Phantom imaging was performed on 3 T prior to patient imaging using the same set-up and imaging protocols. Geometric accuracy in patients and phantoms yielded comparable results for external fiducial reference deviations and internal landmarks between 3 T and 1.5 T MRI (mean ?0.6 mm; standard deviation ?0.3 mm). Mean stereotactic reference deviations ...

2010-11-21

55

Protein Coding Sequence Identification by Simultaneously Characterizing the Periodic and Random Features of DNA Sequences

One of the limiting contributors to the heat load constraint for a long term spent fuel repository is the decay of americium-241. A possible option to reduce the heat load produced by Am-241 is to eliminate it via transmutation in a light water reactor thermal neutron environment, in particular, by taking advantage of the large thermal fission cross section of Am-242 and Am-242m. In this study we employ lattice loading optimization techniques to define the loadings and arrangements of fuel pins with blended americium and uranium oxide in boiling water reactor bundles, specifically, by defining the incineration of pre-loaded americium as an objective function to maximize americium transmutation. Subsequently, the viability of these optimized lattices is tested by assembling them into bundles with Am-spiked fuel pins and by loading these bundles into realistic three-dimensional BWR core-wide simulations that model multiple reload cycles and observe standard operational constraints. These ...

2010-02-01

56

Los Alamos sequence analysis package for nucleic acids and proteins.

Most codon indices used today are based on highly biased nonrandom usage of codons in coding regions. The background of a coding or noncoding DNA sequence, however, is fairly random, and can be characterized...Full Text Available

2005-01-01

57

High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.

An interactive system for computer analysis of nucleic acid and protein sequences has been developed for the Los Alamos DNA Sequence Database. It provides a convenient way to search or verify various...Full Text Available

1982-01-11

58

DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry

We describe a non-isotopic, semi-automated method for large-scale multiplex analysis of nucleic acid sequences, using the cystic fibrosis transmembrane regulator (CFTR) gene as an example. Products...Full Text Available

1994-10-25

59

Actin-like sequences are present on human X and Y chromosomes.

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) has been explored widely for DNA sequencing. The major requirement for this method is that the DNA sequencing fragments...Full Text Available

2001-11-01

60

X-ray production yield in standardized thick target PIXE

The human genome contains greater than 20 actin-related sequences, six of which at least are expressed as protein. We have shown by blot hybridization the presence of actin-like sequences on both the...Full Text Available

1984-08-01

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61

TARGET SIGNAL DETECTOR AND DETECTING METHOD

No abstract prepared.

2003-07-01

62

J-STORE (Japan)

Full Text Available

2005-12-02

63

Prospects for polarized target materials with pure carbon background.

Computational Challenges in miRNA Target Predictions: To Be or Not to Be a True Target?

No abstract available.

1992-01-01

64

APPARATUS OF MAKING UP MEASUREMENT TARGET

All microRNA (miRNA) target—finder algorithms return lists of candidate target genes. How valid is that output in a biological setting? Transcriptome analysis has proven to be a useful approach...Full Text Available

2009-01-01

65

J-STORE (Japan)

Full Text Available

2007-10-26

66

Time-dependent ARMA modeling of genomic sequences

Stellar evolution. II - The evolution of a 3 sun-mass star from the main sequence through core helium burning.

BackgroundOver the past decade, many investigators have used sophisticated time series tools for the analysis of genomic sequences. Specifically, the correlation of the nucleotide...Full Text Available

67

Genome analysis with inter-nucleotide distances

Three Sun-mass star evolution from main sequence to helium exhaustion in core, noting chronology of

1965-01-01

68

Compressing DNA sequence databases with coil

Motivation: DNA sequences can be represented by sequences of four symbols, but it is often useful to convert the symbols into real or complex numbers for further analysis. Several mapping...Full Text Available

2009-12-01

69

{sup 13}C-NMR signal enhancement by using parahydrogen induced polarization (PGIP) and appropriate pulse sequences

BackgroundPublicly available DNA sequence databases such as GenBank are large, and are growing at an exponential rate. The sheer volume of data being dealt with presents serious...Full Text Available

70

Secondary Structure Analysis of a G-rich Sequence Recognizing RNA Aptamer with Structure Specific Enzymes

No abstract prepared.

2009-07-01

71

Congenital Inner Ear Malformation: Three Dimensional Volume Rendering Image Using MR CISS Sequence

Full Text Available

2011-06-01

72

CloVR: A virtual machine for automated and portable sequence analysis from the desktop using cloud computing.

Full Text Available

2003-10-01

73

2009 Community Sequencing Program: Life Under Ice

PMID:21878105

2011-08-30

74

ScienceCinema

...carbon dioxide in the atmosphere contributes to global warming anything that could ...

75

The use of molecular biology techniques for the diagnosis and epidemiological study of foot-and-mouth disease virus in Thailand

Assembly sequencing with toleranced parts

The detection of foot-and-mouth disease (FMD) virus from various kinds of field samples (tissue extract and cell culture isolate) was studied using the polymerase chain reaction (PCR) technique. The gene selected for diagnosis was the polymerase gene and an amplification target product of 454 bp in length was produced using AP5/AP6 primer sets. The PCR product was further examined by NcoI endonuclease digestion. The presence of the internal restriction site was confirmed by demonstration of two small fragments of 330 bp and 124 bp in length. Forty-nine samples that gave positive and negative results by ELISA typing and were positive by the PCR test were tested by NcoI digestion to confirm the results. About 10% of PCR products could not be confirmed by the method. Furthermore the FMD RNA polymerase gene could be detected by the PCR method in samples negative in both ELISA typing and the virus isolation test. A total of 23 samples were examined and compared after ...

2000-05-01

76

Targeting Prostate Cancer Cells In Vivo Using a Rapidly Internalizing Novel Human Single-Chain Antibody Fragment

The goal of assembly sequencing is to plan a feasible series of operations to construct a product from its individual parts. Previous research has thoroughly investigated assembly sequencing under the assumption that parts have nominal geometry. This paper considers the case where parts have toleranced geometry. Its main contribution is an efficient procedure that decides if a product admits an assembly sequence with infinite translations that is feasible for all possible instances of the components within the specified tolerances. If the product admits one such sequence, the procedure can also generate it. For the cases where there exists no such assembly sequence, another procedure is proposed which generates assembly sequences that are feasible only for some values of the toleranced dimensions. If this procedure produces no such sequence, ...

1995-02-21

77

Mean energies of photoneutron spectra for sup(67,70)Zn, sup(74,77)Se, sup(84,86,87,88)Sr, sup(181)Ta and sup(183)W isotopes

Human antibodies targeting prostate cancer cell surface epitopes may be useful for imaging and therapy. The objective of this study was to evaluate the tumor targeting of an internalizing human...Full Text Available

2010-03-01

78

Isoform-level microRNA-155 target prediction using RNA-seq

... reactions selenium 74 target selenium 77 target strontium 84 target strontium

1986-04-15

79

Delineating Bacteriostatic and Bactericidal Targets in Mycobacteria Using IPTG Inducible Antisense Expression

Computational prediction of microRNA targets remains a challenging problem. The existing rule-based, data-driven and expression profiling approaches to target prediction are mostly approached from the...Full Text Available

2011-05-01

80

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit gene: Cloning, mapping, structure, and targeting in mouse

In order to identify novel high value antibacterial targets it is desirable to delineate whether the inactivation of the target enzyme will lead to bacterial death or stasis. This knowledge is particularly...Full Text Available

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81

THE ACS LCID PROJECT. III. THE STAR FORMATION HISTORY OF THE CETUS dSph GALAXY: A POST-REIONIZATION FOSSIL

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit is a member of a family of ligand-gated ion channels, and is the only subunit know to bind {alpha}-bungarotoxin in mammalian brain. {alpha}-Bungarotoxin binding sites are known to be more abundant in the hippocampus of mouse strains that are particularly sensitive to nicotine-induced seizures. The {alpha}7 receptor is highly permeable to calcium, which could suggest a role in synaptic plasticity in the nervous system. Auditory gating deficiency, an abnormal response to a second auditory stimulus, is characteristic of schizophrenia. Mouse strains that exhibit a similar gating deficit have reduced hippocampal expression of the {alpha}7 subunit. We have cloned and sequenced the full length cDNA for the mouse {alpha}7 gene (Acra-7) and characterized its gene structure. The murine {alpha}7 shares amino acid identity of 99% and 93% with the rat and human {alpha}7 subunits, respectively. Using an interspecies ...

1994-09-01

82

Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells

We use deep HST/ACS observations to calculate the star formation history (SFH) of the Cetus dwarf spheroidal (dSph) galaxy. Our photometry reaches below the oldest main-sequence turnoffs, which allows us to estimate the age and duration of the main episode of star formation in Cetus. This is well approximated by a single episode that peaked roughly 12 #+-# 0.5 Gyr ago and lasted no longer than about 1.9 #+-# 0.5 Gyr (FWHM). Our solution also suggests that essentially no stars formed in Cetus during the past 8 Gyr. This makes Cetus' SFH comparable to that of the oldest Milky Way dSphs. Given the current isolation of Cetus in the outer fringes of the Local Group, the dominant old population implies that Cetus is a clear outlier in the morphology-Galactocentric distance relation that holds for the majority of the Milky Way dwarf satellites. Our results also show that Cetus continued forming stars until z#approx =# 1, long after the universe was reionized, and that ...

2010-09-10

83

Optimization of americium-loaded lattices tested in 3D BWR core-wide simulations

Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene. The plasmid pEGFP-optS, which contains the codon-optimized SARS-CoV S gene and expresses spike-EGFP fusion protein (S-EGFP) as silencing target and expressing reporter, was transfected with siRNAs into HEK 293T cells. At various time points of posttransfection, the levels of S-EGFP expression and amounts of spike mRNA transcript were detected by fluorescence microscopy, flow cytometry, Western blot, and real-time quantitative PCR, respectively. The results showed that the cells transfected with pEGFP-optS expressed S-EGFP fusion protein at a higher level compared with those transfected with pEGFP-S, which contains wildtype SARS-CoV spike gene sequence. The green fluorescence, mean fluorescence intensity, and ...

2004-11-26

84

Developement of integrated evaluation system for severe accident management

One of the limiting contributors to the heat load constraint for the Yucca Mountain repository is the decay of Americium 241. A possible option to reduce the heat load produced by Am-241 is to eliminate or transmute it in a light water reactor thermal neutron environment, particularly, by taking advantage of the thermal fission cross section of Am-242 and Am-242m. In this study we employ lattice loading optimization techniques to define the americium/uranium blending and pin arrangements via an adaptation of the code FORMOSA-L to include the incineration of preloaded americium as an objective function. The optimization routines were designed to maximize americium transmutation, while maintaining power peaking below a predefined constraint. The viability of these lattice designs has been analyzed by creating bundles with these Am-spiked lattices and by loading these bundles into realistic 3D BWR core-wide simulation models over multiple reload cycles, a task that has been possible via ...

2008-09-14

85

Coordinated responses of phytochelatin synthase and metallothionein genes in black mangrove, Avicennia germinans, exposed to cadmium and copper

The scope of the project includes four activities such as construction of DB, development of data base management tool, development of severe accident analysis code system and FP studies. In the construction of DB, level-1,2 PSA results and plant damage states event trees were mainly used to select the following target initiators based on frequencies: LLOCA, MLOCA, SLOCA, station black out, LOOP, LOFW and SGTR. These scenarios occupy more than 95% of the total frequencies of the core damage sequences at KSNP. In the development of data base management tool, SARD 2.0 was developed under the PC microsoft windows environment using the visual basic 6.0 language. In the development of severe accident analysis code system, MIDAS 1.0 was developed with new features of FORTRAN-90 which makes it possible to allocate the storage dynamically and to use the user-defined data type, leading to an efficient memory treatment and an easy understanding. Also for ...

86

Coordinated responses of phytochelatin synthase and metallothionein genes in black mangrove, Avicennia germinans, exposed to cadmium and copper

To evaluate the role of phytochelatins and metallothioneins in heavy metal tolerance of black mangrove Avicennia germinans, 3-month-old seedlings were exposed to cadmium or copper for 30 h, under hydroponic conditions. Degenerate Mt2 and PCS primers were synthesized based on amino acid and nucleotide alignment sequences reported for Mt2 and PCS in other plant species found in GenBank. Total RNA was isolated from A. germinans leaves and two partial fragments of metallothionein and phytochelatin synthase genes were isolated. Gene expression was evaluated with reverse transcripatase-polymerase chain reaction (RT-PCR) amplification technique. Temporal analysis showed that low Cd{sup 2+} and Cu{sup 2+} concentrations caused a slight (but not significant) increase in AvMt2 expression after a 16 h exposure time, while AvPCS expression showed a significant increase under the same conditions but only after 4 h. Results strongly suggest that the rapid increase in AvPCS ...

2007-08-01

87

ASTEC and MELCOR comparison for a VVER-1000 60 mm small break LOCA

To evaluate the role of phytochelatins and metallothioneins in heavy metal tolerance of black mangrove Avicennia germinans, 3-month-old seedlings were exposed to cadmium or copper for 30 h, under hydroponic conditions. Degenerate Mt2 and PCS primers were synthesized based on amino acid and nucleotide alignment sequences reported for Mt2 and PCS in other plant species found in GenBank. Total RNA was isolated from A. germinans leaves and two partial fragments of metallothionein and phytochelatin synthase genes were isolated. Gene expression was evaluated with reverse transcripatase-polymerase chain reaction (RT-PCR) amplification technique. Temporal analysis showed that low Cd"2"+ and Cu"2"+ concentrations caused a slight (but not significant) increase in AvMt2 expression after a 16 h exposure time, while AvPCS expression showed a significant increase under the same conditions but only after 4 h. Results strongly suggest that the rapid increase in AvPCS expression ...

2007-08-01

88

A thermal hydraulic investigation on ADSR liquid lead target

In this paper a comparison between severe accident calculations performed for a WWER 1000 with the ASTEC1.1v0 and MELCOR 1.8.5 computer codes for a small break LOCA (ID 60 mm) without intervention of hydro accumulators is presented. This investigation has been performed in the framework of the SARNET project under the EURATOM 6th framework program. Once the accident sequence scenario is specified, both codes (MELCORE and ASTEC) are able to determine the core and containment damaged states, to estimate the release of radionuclides from the fuel as well as from the primary circuit and containment. Theses results are used to estimate the maximum period of the time during which the personnel could still take particular decisions in order to mitigate such an accident. The aim of the performed analysis is to estimate the discrepancy between ASTEC and MELCORE 1.8.5 calculations. Such discrepancies will be studied, if the case, proposal for ASTEC improvements will be made. ...

2005-06-08

89

The response of small scale rigid targets to shallow buried explosive detonations

Computational fluid dynamics(CFD) code FLUENT was used to simulate the thermal hydraulic processes occurring in conceptual design of the accelerator-driven subcritical reactor(ADSR) liquid lead target. The purpose of the analysis is to investigate the thermal hydraulic characteristics of liquid lead as ADSR target material with various target geometries and injection locations of proton beam. In the calculation analysis, the local temperature of the liquid lead target rises to the boiling temperature very rapidly. When the proton beam is injected from the bottom of the target system, the duration time to reach the boiling temperature is longer and the temperature distribution is flatter than other cases.

1998-05-01

90

British Library Electronic Table of Contents (United Kingdom)

Experimental and computational investigations were performed in order to better understand the mechanical response of rigid targets with various geometries to the detonation of shallow buried explosives. The motion of the targets was measured by use of high-speed digital video photography. This work involved flat targets, targets that were downwardly convex, and targets that were downwardly concave with explosive charges located at various positions beneath the targets. It was observed that, in general, angled hulls - whether downwardly concave or convex - tended to reduce the amount of momentum imparted to the center of mass of the targets. Computations were performed by use of an arbitrary Langrangian-Eulerian treatment in a nonlinear finite element code. A model based on quasi-static te...

2011-01-01

91

Targeting a Ruthenium Complex to the Nucleus with Short Peptides

Target stations and beam dumps for the CERN SPS

In an effort to develop octahedral metal complexes as chemotherapeutic and diagnostic agents targeted to DNA, it is critical to optimize the properties of their cellular uptake. Appending d-octaarginine...Full Text Available

2010-05-15

92

Suitability of a New Plant Invader as a Target for Biological Control in Florida

The design of the absorber blocks for internal and external dumping of the SPS proton beam is discussed. In addition, the external target stations for slow and fast extracted proton beams are described.

1977-03-16

93

Nato SCI-12 on Camouflage Evaluation Workshop on Search and Target ...

... L. Sutton. 2000. Is the aquatic weed hygrophila, Hygrophila polysperma (Polemoniales: Acanthaceae), a suitable target for classical biological ... ...

94

FOR THIN AND THICK TARGETS - NASA Technical Report Server (NTRS)

Nato SCI-12 on Camouflage Evaluation Workshop on Search and Target Acquisition The Netherlands, June 1999. Image Discrimination Models for Object Detection ...

95

Target area chamber system design for the National Ignition Facility

By W. Wayne Scott. Langley Research Center. SUMMARY. Thin- and thick-target bremsstrahlung spectra are presented for electron energies up to 7.0 MeV. ...

96

The viroid and viroid-like RNA database.

The National Ignition Facility (NIF) is a proposed Department of Energy facility which will contribute to the resolution of important Defense Program and inertial fusion energy issues for energy production in the future. The NIF will consist of a laser system with 192 independent beamlets transported to a target chamber. The target chamber is a multi-purpose structure that provides the interface between the target and the laser optics. The chamber must be capable of achieving moderate vacuum levels in reasonable times; it must remain dimensionally stable within micron tolerances, provide support for the optics, diagnostics, and target positioner; it must minimize the debris from the x-ray and laser light environments; and it must be capable of supporting external neutron shielding. The chamber must also be fabricated from a low neutron activation material. The fusion reaction in the ...

1994-06-19

97

The ovalbumin gene family: complete sequence and structure of the Y gene.

The viroid and viroid-like RNA database is a compilation of all natural sequences published in journals or available from the GenBank and EMBL nucleotide sequence libraries. Several information regarding...Full Text Available

1998-01-01

98

The comparative structure of mammalian glyceraldehyde 3-phosphate dehydrogenase

The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin...Full Text Available

1982-07-24

99

Sequence space coverage, entropy of genomes and the potential to detect non-human DNA in human samples

1. The amino acid sequences around the thiol groups of glyceraldehyde 3-phosphate dehydrogenase from badger and monkey skeletal muscle were compared with the sequences around the thiol groups in the...Full Text Available

1969-01-01

100

Sequence features that drive human promoter function and tissue specificity

BackgroundGenomes store information for building and maintaining organisms. Complete sequencing of many genomes provides the opportunity to study and compare global information properties...Full Text Available

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101

SEQADAPT: an adaptable system for the tracking, storage and analysis of high throughput sequencing experiments

Promoters are important regulatory elements that contain the necessary sequence features for cells to initiate transcription. To functionally characterize a large set of human promoters, we measured...Full Text Available

2010-07-01

102

Molecular cloning, cDNA sequence, and chromosomal assignment of the human radixin gene and two dispersed pseudogenes

BackgroundHigh throughput sequencing has become an increasingly important tool for biological research. However, the existing software systems for managing and processing these data...Full Text Available

103

Molecular cloning and nucleotide sequence of the beta-lytic protease gene from Achromobacter lyticus.

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin. The authors have cloned and sequenced the human radixin cDNA and found the predicted amino acid sequence for the human protein to be nearly identical to those predicted for radixin in the two other species. By Southern analyses of Chinese hamster x human somatic cell hybrid DNA and of PCR products derived from hybrids, the coding gene (RDX) was mapped to 11q. Fluorescence chromosomal in situ hybridization with a cDNA plasmid further localized this gene to band 11q23. However, PCR amplification with [open quotes]radixin-specific[close quotes] primers on the hybrid DNA panel yielded an additional, very similar DNA sequence that was further characterized by direct sequencing of PCR products. This ...

1993-04-01

104

Massive turnover of functional sequence in human and other mammalian genomes

Two bacteriolytic enzymes secreted by Achromobacter lyticus M497-1 were purified and identified as being very similar (considering their amino acid composition and N-terminal sequence) to alpha- and...Full Text Available

1990-11-01

105

Left ventricular volumetry by nuclear magnetic resonance imaging: the interest of the phased array antenna and of the segmented gradient echo sequences; Volumetrie ventriculaire gauche en IRM: interet des sequences d`echo de gradient segmentees et de l`antenne phased array

Despite the availability of dozens of animal genome sequences, two key questions remain unanswered: First, what fraction of any species' genome confers biological function, and second, are apparent...Full Text Available

2010-10-01

106

Isolation, sequence analysis, and physiological properties of enkephalins in the nervous tissue of the shore crab Carcinus maenas L.

Short communication

1995-10-01

107

Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies

[Leu]- and [Met]enkephalin from thoracic ganglia of the shore crab Carcinus maenas have been purified to homogeneity by a reversed-phase HPLC procedure. Automated gas-phase sequencing revealed a primary...Full Text Available

1991-10-01

108

Improved DNA Sequencing Accuracy and Detection of Heterozygous Alleles Using Manganese Citrate and Different Fluorescent Dye Terminators

The spliced alignment of expressed sequence data to genomic sequence has proven a key tool in the comprehensive annotation of genes in eukaryotic genomes. A novel algorithm was developed to assemble...Full Text Available

2003-10-01

109

The use of dideoxynucleotide triphosphates labeled with different fluorescent dyes (dye terminators) is the most versatile method for automated DNA sequencing. However, variation in peak heights reduces...Full Text Available

1999-06-01

110

Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum

BackgroundA large fraction of the human genome is attributable to L1 retrotransposon sequences. Not only do L1s themselves make up a significant portion of the genome, but L1-encoded...Full Text Available

2003-01-01

111

Evolution of the nuclear ribosomal DNA intergenic spacer in four species of the Daphnia pulex complex

The genome sequence of the solvent-producing bacterium Clostridium acetobutylicum ATCC 824 has been determined by the shotgun approach. The genome consists of a 3.94-Mb chromosome and...Full Text Available

2001-08-01

112

Detection of Atopobium vaginae in Postmenopausal Women by Cultivation-Independent Methods Warrants Further Investigation

BackgroundConcerted evolution refers to the pattern in which copies of multigene families show high intraspecific sequence homogeneity but high interspecific sequence diversity....Full Text Available

113

Completely phased genome sequencing through chromosome sorting

We sequenced 16S rRNA genes from the vaginal swab contents of a postmenopausal woman with asymptomatic bacterial vaginosis (BV). Sequences from Atopobium vaginae were the most commonly...Full Text Available

2004-04-01

114

Complete plastid genome sequences suggest strong selection for retention of photosynthetic genes in the parasitic plant genus Cuscuta

The two haploid genome sequences that a person inherits from the two parents represent the most fundamentally useful type of genetic information for the study of heritable diseases and the development...Full Text Available

2011-01-04

115

Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants.

BackgroundPlastid genome content and protein sequence are highly conserved across land plants and their closest algal relatives. Parasitic plants, which obtain some or all of their...Full Text Available

116

An excess of rare genetic variation in ABCE1 among Yorubans and African-American individuals with HIV-1

The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of...Full Text Available

1997-02-01

117

A proposal to sequence the genome of a garter snake (Thamnophis sirtalis)

Signatures of natural selection occur throughout the human genome and can be detected at the sequence level. We have re-sequenced ABCE1, a host candidate gene essential for...Full Text Available

2009-12-01

118

A Software System for Data Analysis in Automated DNA?Sequencing

Here we develop an argument in support of sequencing a garter snake (Thamnophis sirtalis) genome, and outline a plan to accomplish this. This snake is a common, widespread, nonvenomous...Full Text Available

119

A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence.

Software for gel image analysis and base-calling in fluorescence-based sequencing consisting of two primary programs, BaseFinder and GelImager, is described. BaseFinder is a framework for trace processing,...Full Text Available

1998-06-01

120

5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.

A DNA fragment located on the 3' side of the Coxiella burnetii htpAB operon was determined by Southern blotting to exist in approximately 19 copies in the Nine Mile I genome. The DNA sequences of this...Full Text Available

1992-09-01

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121

Handbook: Approaches for the Remediation of Federal Facility ...

Synthetic complementary oligonucleotides are useful hybridization probes for the detection of mRNAs and genes encoding proteins for which only a partial amino acid sequence is known. Usually this involves...Full Text Available

1988-03-01

122

Genomics - from Neanderthals to high-throughput sequencing

... 4-4 UXO disposal operations ... testing of sequencing batch reactor treatment of ... and lead toward the anode compartment ..... ...

1993-09-01

123

Flip-flop sequence for restoring Zeeman order in the laboratory reference frame

A report on 'The Biology of Genomes' meeting, Cold Spring Harbor, USA, 10-14 May 2006.

2006-01-01

124

Chromosomal localization and cDNA sequence of human BTEB, a GC box binding protein

... spin-lattice relaxation angular momentum electronic circuits magnetic

125

Automated purification of Borrelia burgdorferi s.l. PCR products with KingFisher"T"M magnetic particle processor prior to genome sequencing

Human BTEB cDNA clones have been isolated, sequenced, and the corresponding gene has been assigned to human chromosome 9, region q13, by fluorescent in situ hybridization and DNA blot analysis using DNAs from hybrid cell clones containing a single human chromosome. The cDNA clone encodes a polypeptide of 244 amino acids whose sequence shows a high sequence similarity with the rat BTEB (98% amino acid identity).

1993-09-01

126

Sequence and Organization of pXO1, the Large Bacillus anthracis Plasmid Harboring the Anthrax Toxin Genes

Borrelia burgdorferi sensu lato genospecies were differentiated by PCR-based sequencing of the borrelial flagellin gene. To evaluate the usefulness of KingFisher"T"M magnetic particle processor in PCR product purification, borrelia PCR products were purified with KingFisher"T"M magnetic particle processor prior to cycle sequencing and the quality of the sequence data received was analyzed. KingFisher was found to offer a rapid and reliable alternative for borrelial PCR product purification.

127

Repetitive satellite-like sequences are present within or upstream from 3 avian protein-coding genes.

The Bacillus anthracis Sterne plasmid pXO1 was sequenced by random, “shotgun” cloning. A circular sequence of 181,654 bp was generated. One hundred forty-three open...Full Text Available

1999-10-01

128

On the spectral sequence from Khovanov homology to Heegaard Floer homology

Peculiar DNA sequences made up by the tandem repetition of a 5 bp unit have been identified within or upstream from three avian protein-coding genes. One sequence is located within an intron of the...Full Text Available

1983-03-11

129

Nucleotide sequence of a Euglena gracilis chloroplast gene coding for the 16S rRNA: homologies to E. coli and Zea mays chloroplast 16S rRNA.

Ozsvath and Szabo show that there is a spectral sequence whose E^2 term is the reduced Khovanov homology of L, and which converges to the Heegaard Floer homology of the (orientation reversed) branched double cover of S^3 along L. We prove that the E^k term of this spectral sequence is an invariant of the link L when k>2. If L is a transverse link, then we show that Plamenevskaya's transverse invariant gives rise to a transverse invariant of L in each of these higher terms.

2008-01-01

130

Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase.

The nucleotide sequence of 16S rDNA from Euglena gracilis chloroplasts has been determined representing the first complete sequence of an algal chloroplast rRNA gene. The structural part of the 16S...Full Text Available

1982-10-25

131

Moderate deviations for stationary sequences of Hilbert valued bounded random variables

A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named...Full Text Available

1991-10-01

132

Isolation, sequence, and expression in Escherichia coli of an unusual thioredoxin gene from the cyanobacterium Anabaena sp. strain PCC 7120.

In this paper, we derive the moderate deviation principle for stationary sequences of bounded random variables with values in a Hilbert space. The conditions obtained are expressed in terms of martingale-type conditions. The main tools are martingale approximations and a new Hoeffding inequality for non adpated sequences of Hilbert-valued random variables. Applications to Cramer-Von Mises statistics, functions of linear processes and stable Markov chains are given.

2008-01-01

133

Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.

Two sequences with homology to a thioredoxin oligonucleotide probe were detected by Southern blot analysis of Anabaena sp. strain PCC 7120 genomic DNA. One of the sequences was shown to code for a protein...Full Text Available

1989-01-01

134

Influence of rotation and magnetic field on the minimum mass of a main-sequence star

The nucleotide sequence of the 5'-terminal oligonucleotides produced by pancreatic RNase digestion of bacteriophage T3 RNA polymerase (EC 2.7.7.6) transcripts of T3 DNA has been determined. The sequence...Full Text Available

1980-07-01

135

Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels

The influence of rigid-body and differential rotation and of a fine-scale chaotic magnetic field and a poloidal magnetic field on the minimum mass of a main-sequence star is investigated. It is shown that rotation and a magnetic field with an energy equal to 10--20% of the star's gravitational energy increase the minimum mass of a main-sequence star by 1.5--2 times.

1980-11-01

136

Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived...Full Text Available

2009-01-01

137

Target R and D at JAERI

Optical System Design for Thermal Target Recognition by Spiral Scanning [TRSS

We proposed a solid and a mercury target concepts through the preliminary conceptual design. To feasible these concepts, analysis and experimental works are being carried out. This paper introduces an outline of present status of target R and D such as heat transfer augmentation experiments for the solid target, mercury flow tests with a loop of maximum flow rate of 15L/min, flow pattern measurements for a cold source moderator etc. as well as preliminary conceptual design works. (author)

1999-03-01

138

Inelastic scattering and neutron transfer induced by {sup 11}Be on a heavy target nucleus

Full Text Available

2004-12-01

139

Cryogenic heat pipe-target with aluminum condenser for the COSY-TOF experiment

Short communication.

1994-12-31

140

A Design of Position Tracking System for Moving Targets with Multi-Sensors

No abstract prepared.

2002-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

A He-gas Cooled, Stationary Granular Target

Full Text Available

2010-01-01

142

Searching for immunomodulatory sequences in sea bass (Dicentrarchus labrax L.): Transcripts analysis from thymus

In the CERN approach to the design of a neutrino factory, the repetition frequency of the proton beam is high enough to consider stationary solid targets as a viable solution for multi-MW beams. The target consists of high density tantalum spheres of 2 mm diameter which can efficiently be cooled by passing a high mass flow He-gas stream through the voids between the Ta-granules. Very small thermal shocks and stresses will arise in this fine grained structure due to the relatively long burst of 3.3 ms from the SPL-proton linac. In a quadruple target system where each target receives only one quarter of the total beam power of 4 MW, conservative temperature levels and adequate lifetimes of the target are estimated in its very high radiation environment. A conceptual design of the integration of the target into the magnetic horn-pion-collector is presented.

2003-01-01

143

British Library Electronic Table of Contents (United Kingdom)

The thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cDNA library from sea bass (Dicentrarchus labrax), one of the most extensively cultured species in South Mediterranean aquaculture. A total of 1632 ESTs from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ESTs were further processed. The total collection of D. labrax thymus ESTs has been deposited in the EBI-GenBank-DBJ database (GenBank accession numbers from FN565576 to FN566839). The functional classification of ESTs was performed by Gene Ontology and KEGG annotation and, successively, the sequences were analysed using the Immuno...

2010-01-01

144

PDBpaint, a visualization webservice to tag protein structures with sequence annotations

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

2011-01-01

145

High-resolution sequence stratigraphic framework and its characteristic. Case study of C-P coal measures in Hebei

High resolution sequence stratigraphy in China

The framework of time-sequence stratigraphy was established according to the point of view of high-resolution sequence stratigraphy. The development characteristic of the sequence, the distribution pattern of strata, the basin evolution and filling, and the relationship between variations of base level and coal accumulation of epicontinental basin were expounded based on the base-level cycles. The result shows that the C-P coal measures in Hebei is a series of basin deposit of inner epicontinental sea. The formation and evolution and sedimentation of the basin is intimately related to the changes of the basin base-level, which controls the development of the coal-accumulation, forming a clear coal-bearing sequence in the cycle. 9 refs., 3 figs.

2001-07-01

146

Heapable Sequences and Subsequences

Since high resolution sequence stratigraphy was introduced into China by DENG Hong-wen in 1995, it has been experienced two development stages in China which are the beginning stage of theory research and development of theory research and application, and the stage of theoretical maturity and widely application that is going into. It is proved by practices that high resolution sequence stratigraphy plays more and more important roles in the exploration and development of oil and gas in Chinese continental oil-bearing basin and the research field spreads to the exploration of coal mine, uranium mine and other strata deposits. However, the theory of high resolution sequence stratigraphy still has some shortages, it should be improved in many aspects. The authors point out that high resolution sequence stratigraphy should be characterized quantitatively and modelized by computer techniques. (authors)

2008-11-01

147

Cloning of the cDNA and gene for a human D sub 2 dopamine receptor

Let us call a sequence of numbers heapable if they can be sequentially inserted to form a binary tree with the heap property, where each insertion subsequent to the first occurs at a leaf of the tree, i.e. below a previously placed number. In this paper we consider a variety of problems related to heapable sequences and subsequences that do not appear to have been studied previously. Our motivation for introducing these concepts is two-fold. First, such problems correspond to natural extensions of the well-known secretary problem for hiring an organization with a hierarchical structure. Second, from a purely combinatorial perspective, our problems are interesting variations on similar longest increasing subsequence problems, a problem paradigm that has led to many deep mathematical connections. We provide several basic results. We obtain an efficient algorithm for determining the heapability of a sequence, and also prove ...

2010-01-01

148

Authentication of byte sequences

A clone encoding a human D{sub 2} dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D{sub 2} receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

1989-12-01

149

Reservoir anatomy within a sequence stratigraphic framework: The Pennsylvanian Breathitt group of Eastern Kentucky

Algorithms for the authentication of byte sequences are described. The algorithms are designed to authenticate data in the Storage, Retrieval, Analysis, and Display (SRAD) Test Data Archive of the Radiation Effects and Testing Directorate (9100) at Sandia National Laboratories, and may be used in similar situations where authentication of stored data is required. The algorithms use a well-known error detection method called the Cyclic Redundancy Check (CRC). When a byte sequence is authenticated and stored, CRC bytes are generated and attached to the end of the sequence. When the authenticated data is retrieved, the authentication check consists of processing the entire sequence, including the CRC bytes, and checking for a remainder of zero. The error detection properties of the CRC are extensive and result in a reliable authentication of SRAD data.

1991-06-01

150

Precision Measurements of the Cluster Red Sequence using an Error Corrected Gaussian Mixture Model

Detailed quantitative sequence stratigraphic analysis and three-dimensional (3-D) modeling of the fluvio-deltaic Pikeville, Hyden, and four Corners formations of the Breathitt Group, eastern Kentucky, indicate that it is possible to make confident deterministic correlations of sand bodies at offshore development well spacings. The key to high-confidence correlation is the delineation of the 3-D architecture of third-order composite sequences, which are seismically mappable, and the recognition of internal fourth-order sequences, which are stacked into lowstand, transgressive, and highstand sequence sets. Volumetric partitioning of reservoir sand bodies between systems tracts and sequences allows prediction of channel sandstone geometries and channel: crevasse splay ratios. Furthermore, channel-fill type is predictable from the sequence stratigraphy. Within 13 ...

1993-09-01

151

cDNA cloning and sequence analysis of preproendothelin-1 (PPET-1) from salmon, Oncorhynchus keta.

The red sequence is an important feature of galaxy clusters and plays a crucial role in optical cluster detection. Measurement of the slope and scatter of the red sequence are affected both by selection of red sequence galaxies and measurement errors. In this paper, we describe a new error corrected Gaussian Mixture Model for red sequence galaxy identification. Using this technique, we can remove the effects of measurement error and extract unbiased information about the intrinsic properties of the red sequence. We use this method to select red sequence galaxies in each of the 13,823 clusters in the maxBCG catalog, and measure the red sequence ridgeline location and scatter of each. These measurements provide precise constraints on the variation of the average red galaxy populations in the observed frame with redshift. We find that the ...

2009-07-01

152

Performance evaluation for ML sequence detection in ISI channels with Gauss Markov Noise

The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 ...

2006-06-01

153

High-resolution sequence stratigraphy of a Hettangian-Sinemurian parallic succession, Bornholm, Denmark

Inter-symbol interference (ISI) channels with data dependent Gauss Markov noise have been used to model read channels in magnetic recording and other data storage systems. The Viterbi algorithm can be adapted for performing maximum likelihood sequence detection in such channels. However, the problem of finding an analytical upper bound on the bit error rate of the Viterbi detector in this case has not been fully investigated. Current techniques rely on an exhaustive enumeration of short error events and determine the BER using a union bound. In this work, we consider a subset of the class of ISI channels with data dependent Gauss-Markov noise. We derive an upper bound on the pairwise error probability (PEP) between the transmitted bit sequence and the decoded bit sequence that can be expressed as a product of functions depending on current and previous states in the (incorrect) decoded sequence and the ...

2010-01-01

154

High resolution sequence stratigraphy, sedimentology, petrology and reservoir potentials of the Glauconitic member in Westerose and adjacent fields, Alberta, Canada

Sequence stratigraphic interpretation of paralic successions is complicated by the complex interfingering of marine and continental strata. The successions may also include terrestrial extensions of marine parasequences and completely independent lacustrine parasequence analogues. Failure in recognizing the possible interbedding of these two independent parasequence types may lead to construction of sequence stratigraphic schemes based on incompatible data sets. We have studied a Lower Jurassic paralic section from the Baltic island of Bornholm. The Hettangian-Sinemurian Sose Bugt Member (Ronne Formation) of Bornholm includes lacustrine, fluvial and restricted marine, estuarine deposits reflecting the basin-margin position. Biostatigraphic resolution is poor and a sequence stratigraphic interpretation of the paralic succession is far from straightforward. A multidisciplinary approach including facies analysis, recognition ...

1995-04-01

155

Variation in the Definition of Clinical Target Volumes for Pelvic Nodal Conformal Radiation Therapy for Prostate Cancer

This thesis studied the petrology and sequence stratigraphy of the Hoadley barrier, a Glauconitic member in the Westerose field in Alberta. The unit contains gas reserves in an extensive northeast-southwest trending marine complex. A high resolution sequence stratigraphic model was used to understand the facies development of the unit and the diagenetic pathway of the strata. Six high frequency sequences were identified within the Glauconitic member. Each are separated from one another by surfaces of incision or subaerial exposure which sometimes coincide with a flooding surface and erosion. This thesis provided a petrographic characterization and depositional history of each of the 6 sequences. Reservoir quality is developed within the relatively quartz-rich foreshore facies of the G2B sandstones. The G3 chert litharenite to sublitharenite sandstones are excellent gas producers because of their coarser ...

2002-07-01

156

Targeted nanoparticles that deliver a sustained, specific release of paclitaxel to irradiated tumors

PurposeWe conducted a comparative study of Clinical Target Volume (CTV) definition of pelvic lymph nodes by multiple GU radiation oncologists looking at the levels...Full Text Available

2009-06-01

157

Targeted Deletion of Somatotroph Insulin-Like Growth Factor-I Signaling in a Cell-Specific Knockout Mouse Model

To capitalize on the response of tumor cells to ionizing radiation, we developed a controlled-release nanoparticle drug delivery system using a targeting peptide that recognizes a radiation-induced...Full Text Available

2010-06-01

158

Specific genetic modifications of domestic animals by gene targeting and animal cloning

The role of IGF-I in the negative regulation of GH expression and release is demonstrated by in vitro and in vivo models; however, the targets and mechanisms of IGF-I...Full Text Available

2010-05-01

159

Selective imaging of adherent targeted ultrasound contrast agents

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available

160

National Newborn Screening Status Reports

The goal of ultrasonic molecular imaging is the detection of targeted contrast agents bound to receptors on endothelial cells. We propose imaging methods that can distinguish adherent microbubbles...Full Text Available

2007-04-21

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

161

Inhibitors of RecA Activity Discovered by High-Throughput Screening: Cell-Permeable Small Molecules Attenuate the SOS Response in Escherichia coli

... detected (and reported) as a by-product of MRM screening (MS/MS) targeted by Law or Rule ... detected (and reported) as a by-product of MRM screening (MS/MS) targeted by Law or Rule ...

162

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes

The phenomenon of antibiotic resistance has created a need for the development of novel antibiotic classes with non-classical cellular targets. Unfortunately, target-based drug discovery against...Full Text Available

2009-10-01

163

Efficient use of accessibility in microRNA target prediction

Peroxisomes, glyoxysomes, glycosomes, and hydrogenosomes have each been classified as microbodies, i.e., subcellular organelles with an electron-dense matrix that is bound by a single membrane. We investigated...Full Text Available

1991-09-01

164

Coordination of PAD4 and HDAC2 in the regulation of p53 target gene expression

Considering accessibility of the 3′UTR is believed to increase the precision of microRNA target predictions. We show that, contrary to common belief, ranking by the hybridization energy or by...Full Text Available

2011-01-01

165

Concurrent schedule control of human visual target fixations1

Histone Arg methylation and Lys acetylation have been found to cooperatively regulate the expression of p53 target genes. Peptidylarginine deiminase 4 (PAD4) is an enzyme that citrullinates...Full Text Available

2010-05-27

166

Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II

Operant conditioning techniques were applied to the study of how target fixations are controlled by the probability of signal occurrence. In a standard vigilance setting, gaze at three illuminable...Full Text Available

1973-11-01

167

Assessment of nodal target definition and dosimetry using three different techniques: implications for re-defining the optimal pelvic field in endometrial cancer

Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with...Full Text Available

2010-12-27

168

The identification of the 1/2"+[660] proton orbitals at high spins in rare-earth nuclei

Purposes1. To determine the optimal pelvic nodal clinical target volume for post-operative treatment of endometrial cancer. 2. To compare the DVH of different treatment planning...Full Text Available

169

Study on high-resolution sequence stratigraphy framework of uranium-hosting rock series in Qianjiadian sag

Decay sequences based on the 1/2"+[660] proton orbital have been identified in "1"7"1Ta and "1"7"7Re based on spin, parity, and large alignment. This decay sequence is observed higher in energy than predicted in cranking calculations based on modified oscillator potentials. Similarly known 1/2"-[541] decay sequences in these and other neighbouring isotopes are observed lower in energy than predicted. A reduction in the strength of the spin-orbit potential for protons is suggested as a solution to these problem. (orig.).

170

Short term relative sea-level oscillations in Upper Devonian Nisku Formation (Alberta, Canada): application to hydrocarbon exploration and recent discoveries

The ore-hosting Yaojia Formation is composed of a set of braided stream medium-fine grained sediments. Guided by the basic theory of high-resolution sequence stratigraphy, and based on the core observation, the analysis of chemical composition of rocks, and data of natural potential logging and apparent resistivity logging, the authors have set up the high-resolution sequence stratigraphy framework of the ore-hosting Yaojia Formation, and discussed the relation of the stratigraphic structure of the middle cycle, as well as the paleotopography, the micro-facies to the formation of uranium deposit. (authors)

2005-07-01

171

Probabilistic risk assessment course documentation. Volume 5. System reliability and analysis techniques Session D - quantification

The relationship between short term relative sea-level oscillations and the reef, off-reef deposits geometry of an Upper Devonian third order sequence highstand of the Nisku Formation in west central Alberta was studied through the analysis of high resolution sequence stratigraphy. Hydrocarbon generation was reconstructed by total organic carbon values and migration patterns that were dependent on the geometry of three fourth order sequences. This stratigraphic reconstruction provided the key to recent hydrocarbon discoveries such as in the Brazeau southern reef margin.

1997-09-01

172

Multi-frequency binary sequence testing at FFTF [Fast Flux Test Facility

This course in System Reliability and Analysis Techniques focuses on the probabilistic quantification of accident sequences and the link between accident sequences and consequences. Other sessions in this series focus on the quantification of system reliability and the development of event trees and fault trees. This course takes the viewpoint that event tree sequences or combinations of system failures and success are available and that Boolean equations for system fault trees have been developed and are available. 93 figs., 11 tabs.

1985-08-01

173

Identification of the 1/2/sup +/(660) proton orbitals at high spins in rare-earth nuclei

The multi-frequency binary sequence experimental technique has been implemented at the Fast Flux Test Facility for routine surveillance activities. The frequency content of the standard rod-movement sequence has been shown to be sufficient to normalize the data at moderate frequencies. This obviates the need for auxiliary calibration measurements and provides the reactivity worth of the test control rod. Analyses of a series of tests conducted in 1986 illustrate that the rod worths inferred from the tests are consistent with zero-power measurements. Also, the dependence of the prompt feedback time constant on reactor conditions was determined.

1988-09-18

174

Engineering Relative Compression of Genomes

Decay sequences based on the 1/2/sup +/(660) proton orbital have been identified in /sup 171/Ta and /sup 177/Re based on spin, parity, and large alignment. This decay sequence is observed higher in energy than predicted in cranking calculations based on modified oscillator potentials. Similarly known 1/2/sup -/(541) decay sequences in these and other neighbouring isotopes are observed lower in energy than predicted. A reduction in the strength of the spin-orbit potential for protons is suggested as a solution to these problem.

1983-12-08

175

Congestion cost allocation method in a pool model

Technology progress in DNA sequencing boosts the genomic database growth at faster and faster rate. Compression, accompanied with random access capabilities, is the key to maintain those huge amounts of data. In this paper we present an LZ77-style compression scheme for relative compression of multiple genomes of the same species. While the solution bears similarity to known algorithms, it offers significantly higher compression ratios at compression speed over a order of magnitude greater. One of the new successful ideas is augmenting the reference sequence with phrases from the other sequences, making more LZ-matches available.

2011-01-01

176

Three-quasiparticle states in "1"7"7Ta

The congestion cost caused by transmission capacities and voltage limit is an important issue in a competitive electricity market. To allocate the congestion cost equitably, the active constraints in a constrained dispatch and the sequence of these constraints should be considered. A multi-stage method is proposed which reflects the effects of both the active constraints and the sequence. In a multi-stage method, the types of congestion are analysed in order to consider the sequence, and the relationship between congestion and the active constraints is derived in a mathematical way. The case study shows that the proposed method can give more accurate and equitable signals to customers. (Author)

2003-09-01

177

Terrorism Knowledge Base (TKB)

... transitions lutetium 175 target mev range 10-100 multipolarity neutrons parity

178

Surgical Counter-Terrorism: Targeting Individuals as an ...

... they assisted in the 2000 attack on the USS Cole? ... List the types of targets that ETA has attacked. ... was at least partially responsible for the attack. ...

2008-04-01

179

Single-Walled Carbon Nanotubes Targeted to the Tumor ...

... Tanzania, and the attack on the USS COLE. ... vis another has no bearing on the legality of the attack. If the person attacked is a combatant, the use of ...

2003-02-03

180

Review of Vaccinia Virus and Baculovirus Viability Versus ...

... 1981. Retention of diphenyls, terphenyls, phenylalkanes and fluorene on graphitized thermal carbon black. Chromatographia 14:510-514. ...

2009-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

181

Production of polarized negative ion beams by collisional pumping

... hazards. One study targeted the effects of common household disinfectants on virus inactivation (Butcher and Ulaeto, 2005). ...

2008-03-01

182

Production of polarized negative ion beams by ''collisional pumping''

The production of polarized negative ion beams by collisional pumping is described. Collisional pumping utilizes repeated charge changing collisions in a thick electron-spin-polarized gas or vapor target to form a polarized fast atom beam. The polarized fast atom beam is then partially converted into a polarized negative ion beam in a vapor target. Analysis is presented for a hydrogen beam passing through either a thick polarized H atom target or a thick polarized alkali target. Large polarizations and large currents may be possible.

1983-01-01

183

Preparation of carrier-free yttrium-88 from strontium target by solvent extraction

The production of polarized negative ion beams by ''collisional pumping'' is described. Collisional pumping utilizes repeated charge changing collisions in a thick electron-spin-polarized gas or vapor target to form a polarized fast atom beam. The polarized fast atom beam is then partially converted into a polarized negative ion beam in a vapor target. Analysis is presented for a hydrogen beam passing through either a thick polarized H atom target or a thick polarized alkali target. Large polarizations and large currents may be possible.

1984-03-01

184

Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

... carrier-free isotopes chemical preparation gamma spectra isotope production

185

Pairing effect in the nucleon transfer processes in quasi-elastic heavy ion scattering

Full Text Available

2009-10-01

186

Market segmentation and targeting for real time pricing

... range krypton 86 reactions molybdenum 92 target probability quasi-elastic

1987-04-14

187

Fragmentation of nuclei by particles and nuclei of intermediate and high energies

While there is growing interest in Real Time Pricing as a competitive tool, its adoption to date has been constrained by wariness on the part of many utilities and many of their customers. That wariness is not misplaced, for Real Time Pricing is not for everyone. In order for both utilities and their customers to benefit, the pricing system must be properly tailored for, and offered to, the appropriate audience. This paper discusses needs for identifying targets, implications of targeting of Real Time Pricing to various types of customers, and implications for design and marketing of such pricing systems. Examples from utility experiences are provided, and recommendations are offered for development of improved targeting of Real Time Pricing.

1996-03-01

188

Experimental determination of the ion-ion potential in the N=50 target region: a tool to probe ground-state nuclear densities

The present state of investigations into the fragmentation of target nuclei by particles and nuclei of intermediate and high energies is reviewed.

1983-11-01

189

Electron-Induced Luminescence and X-Ray Spectrometer (ELXS) System for Life Detection

No abstract prepared.

1999-07-01

190

Electromagnetic excitations in nuclei: From photon scattering to photo-dissociation

The ELXS concept is a novel, portable, micro-instrument targeted for the detection of mineralogic

2002-01-01

191

Effect of improved target designs on the "2"3"8Pu production at the Fast Flux Test Reactor

... 98 target molybdenum 99 niobium 91 photoneutrons photonuclear reactions

2006-03-20

192

Ultraconserved Elements: Analyses of Dosage Sensitivity, Motifs and Boundaries

This paper present the results of a series of calculations made to determine the "2"3"8Pu production potential of several advanced target assembly designs in the Fast Flux Test Facility (FFTF). These calculations show that by using advanced target designs the intimately mix the "2"3"7Np target material with an yttrium hydride moderator, the FFTF has the potential of producing up to 30 kg of high-quality "2"3"8Pu per year.

1991-11-10

193

The COG database: an updated version includes eukaryotes

Ultraconserved elements (UCEs) are sequences that are identical between reference genomes of distantly related species. As they are under negative selection and enriched near or in specific classes...Full Text Available

2008-12-01

194

Systems medicine: the future of medical genomics and healthcare

BackgroundThe availability of multiple, essentially complete genome sequences of prokaryotes and eukaryotes spurred both the demand and the opportunity for the construction of an...Full Text Available

195

Space power systems prelaunch integration

High-throughput technologies for DNA sequencing and for analyses of transcriptomes, proteomes and metabolomes have provided the foundations for deciphering the structure, variation and function of the...Full Text Available

196

Sirtuins, Bioageing, and Cancer

The sequence of events from the assembly of a space nuclear power system to its integration in the Space Shuttle Transportation System (STS) is considered. First, the sequence followed for SNAP-10A, the only free world space reactor electric power system ever launched and operated in space, is reviewed. Before shipment, the SNAP-10A reactor was raised to operating temperature using electrically supplied heat and operated at low power for control calibration. Next we discuss shipment to the launch site, a phase that is critical because of the potential for various accidents. Once the power system arrives at the launch site, the processing sequence is performed. This sequence includes checkout, mating with the payload or upper stage launch vehicle, and integration into the STS.

197

Sequences of versatile, broad-host-range vectors of the RK2 family.

The Sirtuins are a family of orthologues of yeast Sir2 found in a wide range of organisms from bacteria to man. They display a high degree of conservation between species, in both sequence and function,...Full Text Available

198

Sequence features involved in the mechanism of 3' splice junction wobbling

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.

2003-07-01

199

SIM: Stellar Astrophysics - SIM - NASA

BackgroundAlternative splicing is an important mechanism mediating the diversified functions of genes in multicellular organisms, and such event occurs in around 40-60% of human...Full Text Available

200

Pitch discrimination by ferrets for simple and complex sounds

... and one near-main-sequence star, which will stringently constrain calculations of single-star evolution at high metallicity. Independent of SIM Lite observations ...

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201

Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) Inferred From Ribosomal DNA Sequences and ...

Although many studies have examined the performance of animals in detecting a frequency change in a sequence of tones, few have measured animals' discrimination of the fundamental frequency...Full Text Available

2009-09-01

202

Phylogenetic relationships in Cortinarius, section Calochroi, inferred from nuclear DNA sequences

... 0877:POSTCS]2.0.CO;2 Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) Inferred From Ribosomal DNA ... C...

203

Ovine reference materials and assays for prion genetic testing

BackgroundSection Calochroi is one of the most species-rich lineages in the genus Cortinarius (Agaricales, Basidiomycota) and is widely distributed...Full Text Available

204

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

205

Natural gas annual 1996

NASA Research Announcement: GALEX GI Program Cycle 2 - GALEX - NASA

This document provides information on the supply and disposition of natural gas to a wide audience. The 1996 data are presented in a sequence that follows natural gas from it`s production to it`s end use.

1997-09-01

206

NASA Research Announcement: GALEX GI Program Cycle 1 - GALEX - NASA

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster ...

207

Menstrual Cycle: Basic Biology

These include, but are not limited to: stellar winds and outflows, post-main- sequence star evolution, binary star evolution, globular cluster structure and ...

208

Mapping Equivalence for Symbolic Sequences: Theory and Applications

The basic biology of the menstrual cycle is a complex, coordinated sequence of events involving the hypothalamus, anterior pituitary, ovary, and endometrium. The menstrual cycle with all its...Full Text Available

2008-01-01

209

Magnetic resonance imaging of chronic aortic dissection

Processing of symbolic sequences represented by mapping of symbolic data into numerical signals is commonly used in various applications. It is a particularly popular approach in genomic and proteomic sequence analysis. Numerous mappings of symbolic sequences have been proposed for various applications. It is unclear however whether the processing of symbolic data provides an artifact of the numerical mapping or is an inherent property of the symbolic data. This issue has been long ignored in the engineering and scientific literature. It is possible that many of the results obtained in symbolic signal processing could be a byproduct of the mapping and might not shed any light on the underlying properties embedded in the data. Moreover, in many applications, conflicting conclusions may arise due to the choice of the mapping used for numerical representation of symbolic data. In this paper, we present a novel framework for ...

2009-01-01

210

MR imaging of the internal auditory canal and inner ear at 3T: comparison between 3D driven equilibrium and 3D balanced fast field echo sequences.

17 patients with chronic aortic dissection were examined by MRI. In 12 patients, comparison between gradient echo sequences and SE sequences was possible. Gradient echo sequences, unlike SE sequences, permitted evaluation of flow in the true and false lumen, reliable differentiation between thrombus and flowing blood and clear delination of the intimal flap. An additional comparison between transoesophageal ultrasound and MRT in 15 patients showed significant advantages in favour of MRI. In three patients MRI was able to detect more proximal origins of the dissection. Moreover, MRI allowed evaluation of the major aortic branches and their relation to the dissection; this was not possible with ultrasound. MRI plays an important role in the follow-up of chronic aortic dissections. (orig.).

211

Isolation and characterization of an ornithine aminotransferase-related sequence (OATL3) mapping to 10q26

Full Text Available

2008-06-01

212

Heavy chain of Acanthamoeba myosine IB is a fusion of myosin-like and non-myosin-like sequences

The authors used a near full-length human ornithine [delta]-aminotransferase cDNA, huOAT6, as a probe under low stringency hybridization conditions to identify a new autosomal ornithine [delta]-aminotransferase-related sequence (OATL3). Cloning and characterization of this sequence reveal it to be a partial nonprocessed pseudogene corresponding to exon 3 and flanking intronic sequences of the ornithine [delta]-aminotransferase structural gene. Using somatic cell hybrids and fluorescence in situ hybridization, they mapped OATL3 to 10q26, adjacent to the ornithine [delta]-aminotransferase structural gene locus. 13 refs., 3 figs.

1993-08-01

213

Heavy chain of Acanthamoeba myosine IB is a fusion of myosin-like and non-myosin-like sequences

Acanthamoeba castellanii myosins IA and IB demonstrate the catalytic properties of a myosin and can support analogues of contractile and motile activity in vitro, but their single, low molecular weight heavy chains, roughly globular shapes, and inabilities to self-assemble into filaments make them structurally atypical myosins. The authors present the complete amino acid sequence of the 128-kDa myosin IB heavy chain, which they deduced from the nucleotide sequence of the gene and which reveals that the polypeptide is a fusion of myosin-like and non-myosin-like sequences. Specifically, the amino-terminal approx. 76 kDa of amino acid sequence is highly similar to the globular head sequences of conventional myosins. By contrast, the remaining approx. 51 kDa of sequence shows no similarity to any portion of conventional myosin sequences, ...

1987-10-01

214

GALAXY EVOLUTION EXPLORER (GALEX) - HEASARC - NASA

Acanthamoeba castellanii myosins IA and IB demonstrate the catalytic properties of a myosin and can support analogues of contractile and motile activity in vitro, but their single, low molecular weight heavy chains, roughly globular shapes, and inabilities to self-assemble into filaments make them structurally atypical myosins. The authors present the complete amino acid sequence of the 128-kDa myosin IB heavy chain, which they deduced from the nucleotide sequence of the gene and which reveals that the polypeptide is a fusion of myosin-like and non-myosin-like sequences. Specifically, the amino-terminal #approx# 76 kDa of amino acid sequence is highly similar to the globular head sequences of conventional myosins. By contrast, the remaining #approx# 51 kDa of sequence shows no similarity to any portion of conventional myosin sequences, ...

215

GALAXY EVOLUTION EXPLORER (GALEX) - GALEX - NASA

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster structure ...

216

Fast evolving 18S rRNA sequences from Solenogastres (Mollusca) resist standard PCR amplification and give new insights into mollusk substitution rate heterogeneity

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster structure ...

217

Norwegian Open Research Archives (NORA)

Full Text Available

218

Detection of polychlorinated biphenyl degradation genes in polluted sediments by direct DNA extraction and polymerase chain reaction

Detection of genetic mutations in repetitive DNA sequences in somatic human cells after low-dose irradiation

It was the aim of this study to specifically detect the DNA sequences for the bphC gene, the meta-cleavage enzyme of the aerobic catabolic pathway for biphenyl and polychlorinated biphenyl degradation, in aquatic sediments without prior cultivation of microorganisms by using extraction of total DNA, PCR amplification of bphC sequences, and detection with specific gene probes. The direct DNA extraction protocol used was modified to enhance lysis efficiency. Crude extracts of DNA were further purified by gel filtration, which yielded DNA that could be used for the PCR. PCR primers were designed for conserved regions of the bphC gene from a sequence alignment of five known sequences. The specificity of PCR amplification was verified by using digoxigenin-labeled DNA probes which were located internal to the amplified gene sequence. The detection limit for the bphC gene of Pseudomonas ...

1993-12-01

219

Detecting retroviral sequences in chronic fatigue syndrome.

... 3-89701-147-6 0949-2224 245 p. animal cells dna genetic radiation effects

1998-03-01

220

Consensus sequence L/PKSSLL mimics crucial epitope on Loop III of Taiwan cobra cardiotoxin

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs. Shyh-Ching Lo and Harvey Alter discovered the presence of sequences closely related to XMRV in the blood of 86.5% of patients with CFS [1]. These findings are important because since the initial discovery of XMRV in CFS, several studies have failed to find XMRV in specimens collected from CFS patients. While the current study also did not find XMRV in CFS, Lo et al. did detect sequences that belong to polytropic mouse endogenous retroviruses (PMV), which share considerable similarity with XMRV. Criteria for future studies that will help bring greater clarity to the issue of retroviral sequences in CFS are proposed below. PMID:21994623

2010-11-03

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221

British Library Electronic Table of Contents (United Kingdom)

Phage display is effective in screening peptides that mimic venom's neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected heptapeptide sequences were aligned with known protein sequences of NAV and BMV in GenBank. One of the four consensus sequences, L/PKSSLL, mimicked the crucial epitope on Loop III of Taiwan cobra cardiotoxin that is associated with the venom's lethal potency. In dot blot analysis, several clones showed varying reactivities for NAV monovalent antivenin and lesser cross-reactions with BMV monovalent antivenin. The KSSLLRN-carrying phage occurred four times in selected clones and showed the strongest ...

2009-01-01

222

Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha (Insecta: Hemiptera: Heteroptera)

Cis-regulatory mutations in human disease

BackgroundNucleotide sequences and the gene arrangements of mitochondrial genomes are effective tools for resolving phylogenetic problems. Hemipteroid insects are known to possess...Full Text Available

223

Choosing and Using a Plant DNA Barcode

Cis-acting regulatory sequences are required for the proper temporal and spatial control of gene expression. Variation in gene expression is highly heritable and a significant determinant...Full Text Available

2009-07-01

224

Cancer gene discovery in mouse and man

The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. This approach was successfully...Full Text Available

225

Analysis of the transcriptome of the root lesion nematode Pratylenchus coffeae generated by 454 sequencing technology

AbstractThe elucidation of the human and mouse genome sequence and developments in high-throughput genome analysis, and in computational tools, have made it possible to profile entire...Full Text Available

2009-12-01

226

British Library Electronic Table of Contents (United Kingdom)

To study interactions between plants and plant-parasitic nematodes, several omics studies have nowadays become extremely useful. Since most data available so far is derived from sedentary nematodes, we decided to improve the knowledge on migratory nematodes by studying the transcriptome of the nematode Pratylenchus coffeae through generating expressed sequence tags (ESTs) on a 454 sequencing platform. In this manuscript we present the generation, assembly and annotation of over 325,000 reads from P. coffeae. After assembling these reads, 56,325 contigs and singletons with an average length of 353bp were selected for further analyses. Homology searches revealed that 25% of these sequences had significant matches to the Swiss-prot/trEMBL database and 29% had significant matches in nematode E...

2011-01-01

227

An Application of Supertree Methods to Mammalian Mitogenomic Sequences

A comparison of the X-ray properties of X Per and gamma Cas

Two different approaches can be used in phylogenomics: combined or separate analysis. In the first approach, different datasets are combined in a concatenated supermatrix. In the second, datasets are...Full Text Available

228

A PRELIMINARY PHYLOGENETIC ANALYSIS OF METAPENAEOPSIS (DECAPODA: PENAEIDAE) BASED ON MITOCHONDRIAL DNA SEQUENCES OF ...

The X-ray properties of the main sequence Be stars conclude that they are a widely separated binary system containing an accreting neutron star.

1982-04-01

229

A $G_{4}$ Sequence within PHR1 Promoter Acts as a Gate for Cross-Talks between Damage-Signaling Pathway and Multi-Stress Response

... each species studied are listed in Table 1. Penaeus monodon Fabricius, 1798 (family Penaeidae) and Solenocera koelbeli De Man, ... ...

230

Target space duality II: applications

Full Text Available

2002-09-01

231

Gastrin releasing peptide (GRP) receptor targeted radiopharmaceuticals: A concise update

We apply the framework developed in Target space duality I: general theory. We show that both nonabelian duality and Poisson-Lie duality are examples of the general theory. We propose how the formalism leads to a systematic study of duality by studying few scenarios that lead to open questions in the theory of Lie algebras. We present evidence that there are probably new examples of irreducible target space duality.

2000-09-25

232

Effluent reduction using pinch technology: Targets for reduction and capital costs for mass exchange networks

The gastrin releasing peptide (GRP) receptor is becoming an increasingly attractive target for development of new radiolabeled peptides with diagnostic and therapeutic potential. The attractiveness of the GRP receptor as a target is based upon the functional expression of GRP receptors in several tumors of neuroendocrine origin including prostate, breast, and small cell lung cancer. This concise review outlines some of the efforts currently underway to develop new GRP receptor specific radiopharmaceuticals by employing a variety of radiometal chelation systems.

2003-11-01

233

Development of a new secondary beam separator and a new gas-jet target at Kyushu University

This paper illustrates how the techniques developed by the authors for capital cost targeting of mass exchange networks can be applied to determination of capital investment targets for reduction in effluent for existing systems involving mass exchange. The results is an impact diagram which shows the relationship between effluent reduction and capital investment, indicating a region of limiting return on investment as well as the maximum possible reduction in effluent. (au)

1999-02-01

234

Detection device for high explosives

In order to facilitate observations of low energy nuclear reactions, a new type recoil mass-separator together with a new gas-jet target system is being developed at the tandem accelerator facility in Kyushu University. The expected mass-resolving power of the separator is 220 for a solid angle of 10 msr and the practical thickness of the gas-jet target will exceed 0.1 atm#centre dot#cm for the light elements of H and He. (author).

1994-06-01

235

A Liquid Parahydrogen Target for the Measurement of a Parity-violating Gamma Asymmetry in Polarized Neutron Capture on Protons

A portable fiber optic detector that senses the presence of specific target chemicals by electrostatically attracting the target chemical to an aromatic compound coating on an optical fiber. Attaching the target chemical to the coated fiber reduces the fluorescence so that a photon sensing detector records the reduced light level and activates an appropriate alarm or indicator.

1992-01-01

236

48 CFR 952.226-73 - Energy Policy Act target group certification.

A 16 l liquid parahydrogen target has been developed for a measurement of the parity-violating {gamma}-asymmetry in the capture of polarized cold neutrons on protons in the {rvec n} + p {yields} d + {gamma} reaction by the NPDGamma collaboration. The target system was carefully designed to meet the stringent requirements on systematic effects for the experiment and also to satisfy hydrogen safety requirements. The target was designed to preserve the neutron polarization during neutron scattering on liquid hydrogen (LH{sub 2}), optimize the statistical sensitivity to the {rvec n} + p {yields} d + {gamma} reaction, minimize backgrounds coming from neutron interaction with the beam windows of the target cryostat, minimize LH{sub 2} density fluctuations which can introduce extra noise in the gamma asymmetry signal, and control systematic effects. The target incorporates two mechanical ...

2010-05-01

237

Selection of IFE target materials from a safety and environmental perspective

...2010-10-01 2010-10-01 false Energy Policy Act target group certification...Acquisition Regulations System DEPARTMENT OF ENERGY CLAUSES AND FORMS SOLICITATION...Provisions and Clauses 952.226-73 Energy Policy Act target group...

2010-10-01

238

Mode of action of membrane-disruptive lytic compounds from the marine dinoflagellate Alexandrium tamarense

Target materials for inertial fusion energy (IFE) power plant designs might be selected for a wide variety of reasons including wall absorption of driver energy, material opacity, cost and ease of fabrication. While each of these issues are of great importance, target materials should also be selected based upon their safety and environmental (S and E) characteristics. The present work focuses on the recycling, waste management and accident dose characteristics of potential target materials. If target materials are recycled so that the quantity is small, isotopic separation may be economically viable. Therefore, calculations have been completed for all stable isotopes for all elements from lithium to polonium. The results of these calculations are used to identify specific isotopes and elements that are most likely to be offensive as well as those most likely to be acceptable in terms of their S and E ...

2001-05-21

239

British Library Electronic Table of Contents (United Kingdom)

Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined. We first hypothesized that membrane sterols serve as molecular targets of these lytic compounds, and that differences in sterol composition among donor and target cells may cause insensitivity of Alexandrium and sensitivity of targets to lytic compounds. We investigated Ca^2^+ influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes. The lytic compounds increased permeability of the cell membrane for Ca^2^+ ions even during blockade of Ca^2^+ channels wit...

2011-01-01

240

An identification method of positron production in laser beam interaction with targets

Activation analysis of target debris in the national ignition facility

A simple electromagnetic transport system was constructed to identify very rare positrons produced in a powerful laser beam interaction with a target. Testing experiments were carried out with CO[sub 2]-laser (10[sup 12] W/cm[sup 2]) beam pulses ([tau] = 50 ns, f = 0.01 Hz) focused on the copper target, as well as with a 96 MeV alpha-particle beam irradiated carbon target. The results showed that the developed system could be effectively used for positron identification and evaluation of their energy by means of a time-of-flight method. The computerized system to deal with this problem, together with others related to the power laser beam interaction with targets, has been constructed. (orig.).

1992-10-01

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241

[Improvement of the recognition probability about camouflage target based on BP neural network].

The modeling methods used to compute the neutron-induced activation of target and near-target materials in the NIF facility are presented. A detailed space and energy description of the neutron environment in the different materials is provided. A new capability has been developed to treat in a general way the activation of debris produced in an operational regime of yield and no-yield experiments. First calculations are addressed to analyze the activity of the debris into the target chamber. The contribution of the different components to activity, interior dose rates, and waste disposal rating (WDR) is determined. The importance of the activation coming from primary irradiation in the target, and from secondary irradiation in debris deposited onto the first wall is assessed. Finally, waste hazards of the activated debris when removed out of the chamber and stored are analyzed. (authors)

1999-09-12

242

Traffic and transport in the Dutch National Environmental Outlook 4; Verkeer en vervoer in de Nationale Milieuverkenning 4

Using static Michelson interferometer to get the spectrum information of measurement targets for spectrum identification, under the condition that the interference length is constant, the system can be optimized by BP neural network algorithm for the mixed spectral separation process. Thereby it can realize improving the recognition probability of camouflage target. Collecting the spectrum information in field of view (FOV) by the interferometer and linear array CCD detector, composing the set of mixed spectrum data, with known absorption spectrum of the material as a hidden layer of rules, it used BP neural network to separate the mixed spectrum data. Experiment with different distances, different combinations of mixed background spectrum as the initial data, using steel target (size: 1.5 m x 1.5 m) made of four kinds, the recognition probability of non-camouflage target is about 90% by BP neural ...

2010-12-01

243

The holographic principle and the language of genes

In the Fourth Dutch National Environmental Outlook (NMP, abbreviated in Dutch) of the National Institute of Public Health and the Environment (VROM), published in July 1997, possible future developments in the traffic and transport sector in the Netherlands are described for the period 1995-2020 and also evaluates present Dutch policies. NMP-4 also offers possible solutions for expected environmental problems. This report serves as a background document for the traffic and transport sector. With a view to present Dutch policies, the main conclusions drawn from the Outlook are that: (1) the policy targets for car and lorry use for 2010 will not be met, (2) the target for CO2 emissions from road transport for 2010 will not be met, (3) the NOx emission target for 2010 will be met for cars, but not for trucks, (4) the VOC emission target for 2010 will be met for cars, but not for trucks, and (5) the noise ...

1998-03-01

244

Sequencing and Comparative Genomic Analysis of pK29, a 269-Kilobase Conjugative Plasmid Encoding CMY-8 and CTX-M-3 ?-Lactamases in Klebsiella pneumoniae?

We show that the holographic principle in quantum gravity imposes a strong constraint on life. The degrees of freedom of an organism can be estimated according to the theory of Boolean networks, which is constrained by the entropy bound. Hence we can explain the languages in protein sequences or in DNA sequences. The overall evolution of biological complexity can be illustrated. And some general properties of protein length distributions can be explained by a linguistic mechanism.

2008-01-01

245

SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

A 269-kilobase conjugative plasmid, pK29, from a Klebsiella pneumoniae strain was sequenced. The plasmid harbors multiple antimicrobial resistance genes, including those encoding CMY-8...Full Text Available

2007-08-01

246

Primary structure of streptococcal Pep M5 protein: Absence of extensive sequence repeats

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

247

Molecular identification of CTX-M and ^blaOXY/K1 ?-lactamase genes in Enterobacteriaceae by sequencing of universal M13-sequence tagged PCR-amplicons

Extensive sequence repeats have been observed in a biologically active fragment of type 24 streptococcal M protein, namely Pep M24 [Beachey, E. H., Sayer, J. M. & Kang, A. H. (1978) Proc....Full Text Available

1983-09-01

248

Molecular cloning of chicken metallothionein. Deduction of the complete amino acid sequence and analysis of expression using cloned cDNA

BackgroundPlasmid encoded ^blaCTX-M enzymes represent an important sub-group of class A β-lactamases causing the ESBL phenotype which is increasingly...Full Text Available

249

Molecular characterization of cap3A, a gene from the operon required for the synthesis of the capsule of Streptococcus pneumoniae type 3: sequencing of mutations responsible for the unencapsulated phenotype and localization of the capsular cluster on the pneumococcal chromosome.

A cDNA library was constructed using RNA isolated from the livers of chickens which had been treated with zinc. This library was screened with a RNA probe complementary to mouse metallothionein-I (MT), and eight chicken MT cDNA clones were obtained. All of the cDNA clones contained nucleotide sequences homologous to regions of the longest (375 bp) cDNA clone. The latter contained an open reading frame of 189 bp, and the deduced amino acid sequence indicates a protein of 63 amino acids of which 20 are cysteine residues. Amino acid composition and partial amino acid sequence analyses of purified chicken MT protein agreed with the amino acid composition and sequence deduced from the cloned cDNA. Amino acid sequence comparison establish that chicken MT shares extensive homology with mammalian MTs. Southern blot analysis of chicken DNA indicates that the chicken MT gene is not a part of ...

1988-01-25

250

High-resolution sequence stratigraphy from outcrop study, with the integration of log and seismic data

The complete nucleotide sequence of the cap3A gene of Streptococcus pneumoniae, which is directly responsible for the transformation of some unencapsulated, serotype 3 mutants to the encapsulated phenotype,...Full Text Available

1994-10-01

251

Glutamine-linked and Non-consensus Asparagine-linked Oligosaccharides Present in Human Recombinant Antibodies Define Novel Protein Glycosylation Motifs

The detailed sequence stratigraphic analysis of the siliciclastic-dominated Late Cretaceous sediments (Aren Sandstone and Garumnian red beds, south central Pyrenees, Spain) reveals the repeating disposition of critical elements and controlling mechanisms of cycles and sequences. Our approach integrates (a) hierarchy of unconformity-bounded units, (b) physical expression of boundaries traceable from the continent to the basin, (c) featuring facies and depositional systems, (d) well log and seismic expression, and (e) driving basing-filling mechanisms. A comparison to other active basins is suggested in order to prove the validity beyond the regional scale. Four basin-wide transgressive facies cycles were identified and interpreted as third-order units. The transgressive phase of each cycle is represented by mixed shelf deposits, while regressive periods consists of complex delta systems. The cycles are composed within their regressive phase of ...

1993-09-01

252

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.

We report the presence of oligosaccharide structures on a glutamine residue present in the V_L domain sequence of a recombinant human IgG2 molecule. Residue Gln-106, present in the QGT sequence...Full Text Available

2010-05-21

253

Extended amino acid sequences around the active-site lysine residue of class-I fructose 1,6-bisphosphate aldolases from rabbit muscle, sturgeon muscle, trout muscle and ox liver.

We have isolated the human prointerleukin 1 (proIL-1) beta gene from leukocyte and fetal liver libraries. The nucleotide sequence and its gene organization reveals that the proIL-1 beta gene is composed...Full Text Available

1986-10-24

254

Draft Genome Sequence of Shewanella sp. Strain HN-41, Which Produces Arsenic-Sulfide Nanotubes.

1. Amino acid sequences covering the region between residues 173 and 248 [adopting the numbering system proposed by Lai, Nakai & Chang (1974) Science 183, 1204-1206] were derived for trout (Salmo...Full Text Available

1979-11-01

255

Detection of Atopobium vaginae in postmenopausal women by cultivation-independent methods warrants further investigation.

The dissimilatory metal reducing bacterium Shewanella sp. strain HN-41 was first reported to produce novel photoactive As-S nanotubes via reduction of As(V) and S(2)O(3)(2-) under anaerobic conditions. Here we report the draft genome sequence and annotation of strain HN-41. PMID:21868804

2011-09-01

256

Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli.

We sequenced 16S rRNA genes from the vaginal swab contents of a postmenopausal woman with asymptomatic bacterial vaginosis (BV). Sequences from Atopobium vaginae were the most commonly detected. In a survey of 35 other postmenopausal women, this organism was detected in 44% with BV but not in any subjects deemed healthy. PMID:15071062

2004-04-01

257

Recurrent plot analysis of discharge sequences in tracking test of polybutylene polymers

Nucleotide sequence analysis of the Escherichia coli chromosomal DNA inserted in the plasmid pLC33-5 of the Clarke and Carbon library [Clarke & Carbon (1976) Cell 9, 91-99] revealed the existence...Full Text Available

1989-01-15

258

Isolation of fetal DNA from nucleated erythrocytes in maternal blood

Polymers are required to use in radiation environments as insulation materials. However, they often suffer from tracking failure. There is an increasing demand to evaluate radiation effects on dielectric performance. This paper presents a recurrence plot (RP) approach to analyse surface discharge sequences of gamma-ray irradiated polymer materials based on tracking test. Studying the non-linear characteristics of discharge sequences can assist in understanding the underlying mechanism of the discharge process. Discharge sequences of the test are extended to m-dimensional phase space by using the phase space reconstructed method. As test samples, polybutylene terephthalate (PBT) and polybutylene naphthalate (PBN) were irradiated to 100 kGy and then up to 1 MGy with a dosage rate of 10 kGy h{sup -1} by using a {sup 60}Co gamma source. The tracking tests were carried out according to the test method described in IEC60112. It ...

2008-10-07

259

Homology analyses of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. The authors used monoclonal antibody against the transferrin receptor (TIR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, they were successful in ...

1990-05-01

260

Affinity purification of sequence-specific DNA binding proteins.

Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases were carried out. The amino acid sequences of the chicken and rat enzymes are 67% identical. If conservative substitutions are allowed, 78% of the amino acids are matched. A region of low homologies exists between the functional domains, in particular around amino acid residues 1059-1264 of the chicken enzyme. Homologies between the active sites of chicken and rat and of chicken and yeast enzymes have been analyzed by an alignment method. A high degree of homology exists between the active sites of the chicken and rat enzymes. However, the chicken and yeast enzymes show a lower degree of homology. The DADPH-binding dinucleotide folds of the {beta}-ketoacyl reductase and the enoyl reductase sites were identified by comparison with a known consensus sequence for the DADP- and FAD-binding dinucleotide folds. The active sites ...

1989-11-01

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261

A Systematic Framework for the Construction of Optimal Complete Complementary Codes

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography steps to give Sp1 of an estimated 90% ...

1986-08-01

262

Development and evaluation of a conditionally lethal transgenic pink bollworm

The complete complementary code (CCC) is a sequence family with ideal correlation sums which was proposed by Suehiro and Hatori. Numerous literatures show its applications to direct-spread code-division multiple access (DS-CDMA) systems for inter-channel interference (ICI)-free communication with improved spectral efficiency. In this paper, we propose a systematic framework for the construction of CCCs based on $N$-shift cross-orthogonal sequence families ($N$-CO-SFs). We show theoretical bounds on the size of $N$-CO-SFs and CCCs, and give a set of four algorithms for their generation and extension. The algorithms are optimal in the sense that the size of resulted sequence families achieves theoretical bounds and, with the algorithms, we can construct an optimal CCC consisting of sequences whose lengths are not only almost arbitrary but even variable between sequence families. We ...

2010-01-01

263

Is uniform target dose possible in IMRT plans in the head and neck?

A new area-wide pest control strategy using the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), genetically transformed with a conditionally lethal gene, is under development. Conditional lethality of several transgenic pink bollworm strains was demonstrated in a series of laboratory rearing experiments. Pink bollworms were transformed with genetic constructs using the RIDL technology (Release of Insects with a Dominant Lethal gene) for development of an autocidal biological control system for possible supplement or replacement of radiation based sterile insect release. LA1124 is a lethal construct controlled by a tetracycline repressible transactivator protein (tTA), in which binding of tTA to its specific target sequence tetO drives production of more tTA. In the absence of tetracycline, this leads to lethality by high expression of tTA. When tetracycline is present, tTA does not bind tetO, and so the positive ...

2005-05-09

264

Depositional and dissolutional processes and their resulting thinning patterns within the Middle Devonian Prairie Formation, Williston basin, North Dakota and Montana

Purpose: Various published reports involving intensity-modulated radiotherapy (IMRT) plans developed using automated optimization (inverse planning) have demonstrated highly conformal plans. These reported conformal IMRT plans involve significant target dose inhomogeneity, including both overdosage and underdosage within the target volume. In this study, we demonstrate the development of optimized beamlet IMRT plans that satisfy rigorous dose homogeneity requirements for all target volumes (e.g., #+-#5%), while also sparing the parotids and other normal structures. Methods and Materials: The treatment plans of 15 patients with oropharyngeal cancer who were previously treated with forward-planned multisegmental IMRT were planned again using an automated optimization system developed in-house. The optimization system allows for variable sized beamlets computed using a three-dimensional convolution/superposition dose ...

2002-04-01

265

Computational identification of developmental enhancers:conservation and function of transcription factor binding-site clustersin drosophila melanogaster and drosophila psedoobscura

Within the Williston basin, thickness variations of the Prairie Formation are common and are interpreted to originate by two processes: differential accumulation of salt during deposition and differential removal of salt by dissolution. Unambiguous evidence for each process is rare because the Prairie/Winnipegosis interval is seldom cored within the US portion of the basin. Therefore, indirect methods, using well logs, provide the principal method for identifying characteristics of the two processes. The results of this study indicate that the two processes can be distinguished using correlations within the Prairie Formation. Several regionally correlative brining-upward and probably shoaling-upward sequences occur within the Prairie Formation. Near the basin center, the lowermost sequence is transitional with the underlying Winnipegosis Formation. This transition is characterized by thinly laminated basal carbonates that become increasingly ...

1988-07-01

266

Radioisotope production in the I.Ph.P.E. cyclotron for medical application

Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. Results We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of neighboring genes; the ...

2004-08-06

267

Radioisotope production in the I. Ph. P. E. cyclotron for medical application

The production methods for seven radioisotopes, Ga-67, Sr-85, Pd-103, In-111, Tu-167, Hg-197 and Pb-203, by using a classical 1.5m cyclotron in the Institute of Physics and Power Engineering, Obninsk, USSR, are described. At present, more than 50 cyclotrons in different countries are used for the production of radioisotopes applied to medicine. Radioisotopes are produced with the cyclotron in the I.Ph.P.E. in the form of irradiated targets, which are delivered to Moscow radiopharmaceutical factory, where radiopharmaceuticals are produced on the base of these targets. The cyclotron is operated in two regimes providing the acceleration of protons, deuterons and alpha -particles. Two types of target assemblies are used for irradiation, the one is intended for the internal beam, and the other is for the external beam. The reactions used for the production of seven radioisotopes described above, the types of ...

268

Distortion-invariant color pattern recognition using multiple phase-shifted-reference-based joint transform correlation incorporating synthetic discriminant function

The production methods for seven radioisotopes, Ga-67, Sr-85, Pd-103, In-111, Tm-167, Hg-197 and Pb-203, by using a classical 1.5m cyclotron in the Institute of Physics and Power Engineering, Obninsk, USSR, are described. At present, more than 50 cyclotrons in different countries are used for the production of radioisotopes applied to medicine. Radioisotopes are produced with the cyclotron in the I.Ph.P.E. in the form of irradiated targets, which are delivered to Moscow radiopharmaceutical factory, where radiopharmaceuticals are produced on the base of these targets. The cyclotron is operated in two regimes providing the acceleration of protons, deuterons and alpha -particles. Two types of target assemblies are used for irradiation, the one is intended for the internal beam, and the other is for the external beam. The reactions used for the production of seven radioisotopes described above, the types of ...

1982-01-01

269

Uses of laser optical pumping to produce polarized ion beams

This paper proposes a new pattern recognition system employing optical joint transform correlation (JTC) technique which offers a great number of advantages over similar digital techniques, including very fast operation, simple architecture and capability of updating the reference image in real time. The proposed JTC technique incorporates a synthetic discriminant function (SDF) of the target image estimated from different training images to make the pattern recognition performance invariant to noise and distortion. It then involves four different phase-shifted versions of the same target SDF reference image, which are individually joint transform correlated with the given input scene. When the correlation signals are combined, it produces a single cross-correlation peak corresponding to each potential target present in the given input scene. The proposed technique also includes a fringe-adjusted filter to generate a ...

2011-04-01

270

The HARP experiment first physics results

Laser optical pumping can be used to produce polarized alkali atom beams or polarized alkali vapor targets. Polarized alkali atom beams can be converted into polarized alkali ion beams, and polarized alkali vapor targets can be used to produce polarized H/sup -/ or /sup 3/He/sup -/ ion beams. In this paper the authors discuss how the polarized alkali atom beams and polarized alkali vapor targets are used to produce polarized ion beams with emphasis on the production of polarized negative ion beams.

1983-04-01

271

Separation of carrier-free "1"7"6","1"7"7W and "1"7"6","1"7"7Ta produced in "1"6O irradiated holmium target

The HARP experiment at CERN is performing extensive measurements of hadron production cross sections and secondary particle yields, in the momentum range 1.5-15 GeV/c, over the full solid angle and using a large set of cryogenic and solid targets. First measurements of hadron production cross-sections in the forward region are reported using an aluminium target 5% of an interaction length thick and a proton beam of 12.9 GeV/c. A preliminary analysis in the large angle region of elastic scattering events produced with the cryogenic hydrogen target at 3 GeV/c beam momentum is also presented.

2005-01-01

272

Separation of carrier free "1"7"6","1"7"7W and "1"7"6","1"7"7Ta radionuclides produced in "1"6O activated holmium metal target

Carrier-free radionuclides of tungsten and tantalum, "1"7"6","1"7"7W and "1"7"6","1"7"7Ta have been produced by heavy ion activation of holmium target with 97 MeV "1"6O"5"+ beam. Radiochemical separation scheme has been developed to isolate tungsten and tantalum radionuclides from the holmium target matrix. (author)

2001-11-01

273

Recovery of radioactive thallium isotopes from lead and bismuth targets irradiated by 1-GeV protons

Carrier-free radioisotopes of tungsten, "1"7"6","1"7"7W, and their corresponding daughter radionuclides "1"7"6","1"7"7Ta, have been produced in holmium target by heavy ion activation with "1"6O"5"+ beam. An attempt has been made to separate these carrier-free radionuclides from bulk holmium target through LLX using cation exchanger HDEHP. (author)

2001-02-07

274

Recovery of radioactive thallium isotopes from lead and bismuth targets irradiated by 1-GeV protons

A simple highly efficient procedure has been developed for recovery of thallium radioisotopes from lead and bismuth targets irradiated by 1-GeV protons. The procedure is based on the use of extraction chromatography. The cross-sections have been determined for formation of {sup 200}Pb, {sup 201}Pb, {sup 202m}Pb, and {sup 203}Pb radioisotopes in targets from lead with natural isotopic composition, irradiated by 1-GeV protons.

1995-03-01

275

Preliminary Study of Plasma Stream Interaction with Tungsten Target within RPI-IBIS Facility

A simple highly efficient procedure has been developed for recovery of thallium radioisotopes from lead and bismuth targets irradiated by 1-GeV protons. The procedure is based on the use of extraction chromatography. The cross-sections have been determined for formation of "2"0"0Pb, "2"0"1Pb, "2"0"2"mPb, and "2"0"3Pb radioisotopes in targets from lead with natural isotopic composition, irradiated by 1-GeV protons.

276

Modified-VSIMM algorithm with an application to the naval fire control technology

The paper presents results of experimental research on the interaction of a pulsed plasma-ion stream with a tungsten (W) target. The pulsed hydrogen plasma was produced within the RPI-IBIS (Multi-Rod Plasma Injector) facility at IPJ in Swierk. Measurements were carried out by means of optical spectroscopy and corpuscular diagnostic techniques. For experiments with the W-target the operational conditions (so-called PID mode) were chosen when a clean hydrogen plasma stream was generated. Attention was paid to the identification of WI and WII spectral lines.

2006-01-01

277

British Library Electronic Table of Contents (United Kingdom)

A modified variable structure interacting multiple model (M-VSIMM) estimator for complex hybrid maneuver target tracking is presented. The M-VSIMM could potentially be applied to fire control systems (FCS) used on warships. Target model groups were designed using 3D dimensional dynamic target models. Optimal model group selection logic was proposed, contrary to the activation and termination logic in the original VSIMM. The system will respond faster with optimal model group selection logic. After performing simulations, the tracking performances of the Kalman, ?-?(-?), VDIE, IMM and M-VSIMM filters were compared under various maneuvering conditions.

2011-01-01

278

Experiments to investigate the effects of radiative cooling on plasma jet collimation

Convoy electron production in polycrystalline and monocrystalline targets

Preliminary experiments have been performed to investigate the effects of radiative cooling on plasma jets. Thin (3 um - 5 um) conical shells were irradiated with an intense laser, driving jets with velocities > 100 km/s. Through use of different target materials - aluminium, copper and gold - the degree of radiative losses was altered, and their importance for jet collimation investigated. A number of temporally resoved optical diagnostics was used, providing information about the jet evolution. Gold jets were seen to be narrower than those from copper targets, while aluminium targets produced the least collimated flows.

2010-01-01

279

Computer-assisted rotation and multiple stationary irradiation technique

The velocity distribution of electrons ejected close to the forward direction by 0.8-2 MeV/A ions traversing various solid targets, including a Au monocrystal, is measured in coincidence with emerging charge-selected ions. The velocity spectrum is observed to be independent of outgoing projectile velocity and charge state for polycrystalline targets. Measurements on the Au crystal under channeling conditions show dependences on final charge state, and are tentatively explained by assuming that the main contribution to the production yield comes from the non-channeled fraction of the ions. A simple model for the creation of the forward-ejected electrons is proposed, which accounts for most of the experimental findings.

1980-01-01

280

Centroid and Envelope Dynamics of High-intensity Charged Particle Beams in an External Focusing Lattice and Oscillating Wobbler

A computer-assisted rotation and stationary conformation radiotherapy system with overrunning multileaf collimators has been developed. This system can produce any shape of target volume, including a number of target areas outside the axis of the rotation, regardless of the location of the axis at 360"0 rotation irradiation. In addition, by changing the dose rate and deleting a partial region within a field, multiple stationary irradiation can produce more homogeneous target volumes - thus avoiding excessive irradiation to critical organs - than the rotation technique. (orig.).

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281

Origin of complex quantum amplitudes and Feynman's rules

The centroid and envelope dynamics of a high-intensity charged particle beam are investigated as a beam smoothing technique to achieve uniform illumination over a suitably chosen region of the target for applications to ion-beam-driven high energy density physics and heavy ion fusion. The motion of the beam centroid projected onto the target follows a smooth pattern to achieve the desired illumination, for improved stability properties during the beam-target interaction. The centroid dynamics is controlled by an oscillating "wobbler", a set of electrically-biased plates driven by RF voltage. __________________________________________________

2010-04-28

282

Optimized pulse sequences for the suppression of decoherence in quantum information

Complex numbers are an intrinsic part of the mathematical formalism of quantum theory and are perhaps its most characteristic feature. In this article, we show that the complex nature of the quantum formalism can be derived directly from the assumption that a pair of real numbers is associated with each sequence of measurement outcomes, with the probability of this sequence being a real-valued function of this number pair. By making use of elementary symmetry conditions, and without assuming that these real number pairs have any other algebraic structure, we show that these pairs must be manipulated according to the rules of complex arithmetic. We demonstrate that these complex numbers combine according to Feynman's sum and product rules, with the modulus-squared yielding the probability of a sequence of outcomes.

2010-02-01

283

Molecular Characterization of Coprophilous Fungal Communities Reveals Sequences Related to Root-Associated Fungal Endophytes

The dynamical decoupling (DD) aims at suppressing the decoherence by means of coherent control pulses. Even if devices exist where instantaneous pulses are an adequate approximation, experimentally a finite duration #tau#_p and a bounded amplitude are inevitable. They are the cause of additional errors which can be corrected by designing the pulse shape appropriately. The new pulse has the overall effect of an ideal, instantaneous pulse with the advantage of decoupling the spin (or qubit) from the bath up to the order O(#tau#_p"3). The limitation of the no-go theorem for #pi# pulses is avoided. Hence, the Uhrig sequence (UDD), originally thought for ideal #pi# pulses, works also for bounded control Hamiltonians. Numerical simulations show that concatenated sequences of real pulses are effective against general decoherence.

2010-03-21

284

British Library Electronic Table of Contents (United Kingdom)

This paper reports the use of molecular methods to characterize the coprophilous fungal communities (CFC) that inhabit the dung of four species of mammalian herbivores at two sites, Sevilleta National Wildlife Refuge (SNWR) in New Mexico and Wind Cave National Park (WCNP) in South Dakota. Results reveal that CFC from domesticated cattle (Bos taurus) at SNWR, and bison (Bison bison) and black-tailed prairie dogs (Cynomys ludovicianus) at WCNP were diverse but dominated primarily by members within eight taxonomic orders, including the rarely cultured and anaerobic order Neocallimastigales. In addition, 7.7% (138 of 1,788) of the sequences obtained from all dung samples were at least 97% similar to root-associated fungal (RAF) sequences previously described from blue grama (Bouteloua gracilis...

2011-01-01

285

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA

Human cDNA mapping using fluorescence in situ hybridization

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

1989-03-01

286

High-resolution sequence stratigraphy and its controls on uranium mineralization. Taking middle Jurassic Qingtujing formation in Chaoshui basin as an example

Genetic mapping is approached using the techniques of high resolution fluorescence in situ hybridization (FISH). This technology and the results of its application are designed to rapidly generate whole genome as tool box of expressed sequence to speed the identification of human disease genes. The results of this study are intended to dovetail with and to link the results of existing technologies for creating backbone YAC and genetic maps. In the first eight months, this approach generated 60--80% of the expressed sequence map, the remainder expected to be derived through more long-term, labor-intensive, regional chromosomal gene searches or sequencing. The laboratory has made significant progress in the set-up phase, in mapping fetal and adult brain and other cDNAs, in testing a model system for directly linking genetic and physical maps using FISH with small fragments, in setting up a database, and in establishing the ...

1993-03-04

287

Bioinformatic analysis of BBTV satellite DNA in Hainan

By applying the high-resolution sequence stratigraphy, the different sub-divisions of base-level cycles of Qingtujing Formation, Middle Jurassic in Chaoshui Basin are analyzed in detail. 23 short, 3 middle and 1 long base-level cycles are recognized. On the above basis, the corresponding frameworks of high-resolution sequence stratigraphy have been established in northern and southern sub-basins respectively, and the detailed sedimentary facies of MSC_1_-_3 and the special distribution of Qintujing Formation are discussed. It is pointed out that MSC_2 is the most favorable layer for the localization of sandstone-type uranium deposits. (authors)

2006-06-01

288

British Library Electronic Table of Contents (United Kingdom)

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiatio...

2011-01-01

289

Balance with Unbounded Complexes

Application of integrated high resolution sequence stratigraphy in the Jeanne d`Arc Basin, offshore Newfoundland - an illustration of the systems tracts within the Tithonian to Berriasian Hibernia Sequence

Given a double complex $X$ there are spectral sequences with the $E_2$ terms being either H$_I$ (H$_{II}(X))$ or H$_{II}($H$_I (X))$. But if $H_I(X)=H_{II}(X)=0$ both spectral sequences have all their terms 0. This can happen even though there is nonzero (co)homology of interest associated with $X$. This is frequently the case when dealing with Tate (co)homology. So in this situation the spectral sequences may not give any information about the (co)homology of interest. In this article we give a different way of constructing homology groups of $X$ when H$_I(X)=$H$_{II}(X)=0$. With this result we give a new and elementary proof of balance of Tate homology and cohomology.

2011-01-01

290

Yields of Residual Nuclei from Proton-Irradiated Materials

The Jeanne d`Arc Basin is located 320 km offshore in the Grand Banks of Newfoundland. It is a Mesozoic failed-rift basin with recoverable oil reserves of about 2 billion barrels. Its huge Hibernia Field will be in production by late 1997. The basin has been reappraised using sequence stratigraphic concepts, and renewed exploration in the area is expected. The Upper Jurassic - Lower Cretaceous sedimentary accumulation in the Basin was formed by the extensional tectonics that created the North Atlantic Ocean. The sedimentary packages were placed in chronostratigraphic order by calibrating the biostratigraphy of the Oxfordian to Maastrichtian section to the detailed ammonite zonation from the North Sea.

1997-09-01

291

International Science & Technology Center (ISTC)

Experimental and Theoretical Study of the Residual Nuclide Production in 40-2600 MeV Proton-Irradiated Thin Targets of ADS Basic Materials

292

Tuberculous Granulomas Are Hypoxic in Guinea Pigs, Rabbits, and Nonhuman Primates?

The skeletal muscle ryanodine receptor identified as a molecular target of[3H] azidodantrolene by photoaffinity labeling

Understanding the physical characteristics of the local microenvironment in which Mycobacterium tuberculosis resides is an important goal that may allow the targeting of metabolic processes...Full Text Available

2008-06-01

293

The influence of target backing on ion-beam electron spectra

... 16, 2001 0006-2979 BIORAK v. 40(2) APPLIED LIFE SCIENCES azido

2001-04-01

294

The importance of an accurate target wave function in variational calculations for (e^{+}-H_{2}) scattering

Several different aspects of the influence of the target backing on in-beam electron spectra following compound nuclear reactions induced by accelerated ions at tandem energies irradiating backed targets are discussed in detail. This discussion is illustrated by a few typical examples, such as "1"2C"5"+ and "3"1P"1"0"+ beams at 4 MeV/u bombarding Sn(+Be), Sn(+Au), Pb(+C) backed targets. Moreover, the relative influence of electron backscattering, electron Doppler shift and Doppler broadening as well as #delta#-electron emission on the low energy electron spectra (E_e#<=#100 keV) obtained under such conditions are investigated in the frame of the available experimental data. (orig.).

295

The Growth Cone Cytoskeleton in Axon Outgrowth and Guidance

Using the complex Kohn method, we have calculated variational values of phase shifts and the annihilation parameter, Z_{eff}, for the elastic scattering of positrons by molecular hydrogen. Our results are sensitive to small changes in the accuracy of the wave function representing the target hydrogen molecule. We have developed a systematic approach to demonstrate that, at low positron energies, there are particular forms of the Kohn trial wave function for which the results of variational calculations are not reliable, even when the target wave function accounts for as much as 96.8% of the correlation energy of H_{2}. We find that reliable results can be recovered if our calculations are extended to admit more sophisticated target wave functions accounting for 99.7% of the correlation energy. Remaining discrepancies between theory and experiment are briefly discussed.

2008-01-01

296

The Development of New Methods for Solving the Target ...

Axon outgrowth and guidance to the proper target requires the coordination of filamentous (F)-actin and microtubules (MTs), the dynamic cytoskeletal polymers that promote shape change and locomotion....Full Text Available

297

Target space duality I: general theory

... and 7 survey papers. On the inverse Stefan problem he wrote 3 research papers and 1 survey paper. The papers on inverse ...

1984-07-18

298

Studies of relativistic heavy ion collisions at the AGS (Experiment 814)

We develop a systematic framework for studying target space duality at the classical level. We show that target space duality between manifolds M and M-tilde arises because of the existence of a very special symplectic manifold. This manifold locally looks like MxM-tilde and admits a double fibration. We analyze the local geometric requirements necessary for target space duality and prove that both manifolds must admit flat orthogonal connections. We show how abelian duality, nonabelian duality and Poisson-Lie duality are all special cases of a more general framework. As an example we exhibit new (nonlinear) dualities in the case M=M-tilde=R{sup n}.

2000-09-25

299

Smoking and reproduction: The oviduct as a target of cigarette smoke

This report discusses the experimental setup of experiment 814 at Brookhaven AGS. This experiment involves the collision of silicon ions with target nuclei. The detector systems are discussed primarily. (LSP)

1990-01-01

300

Simultaneous recognition and segmentation of cells: application in C.elegans

The oviduct is an exquisitely designed organ that functions in picking-up ovulated oocytes, transporting gametes in opposite directions to the site of fertilization, providing a suitable environment...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

Principles of antibody therapy.

Motivation: Automatic recognition of cell identities is critical for quantitative measurement, targeting and manipulation of cells of model animals at single-cell resolution. It has been...Full Text Available

2011-10-15

302

Novel Cytotoxic Vectors Based on Adeno-Associated Virus

The success of monoclonal antibodies in clinical practice is dependent on good design. Finding a suitable target is the most important part as other properties of the antibody can be altered by genetic...Full Text Available

1992-12-05

303

Wastenet

positive primary PymT breast cancer cells in primary co-cultured tumor tissue, suggesting target specificity of

304

Measured Temperatures of Solid Rocket Motors Dump Stored ...

... TARGET SENSOR-MK 80 SERIES BOMBS ... 5-inch-, and 2.75-inch- diameter rocket motors that ... balance when compared to incoming solar radiation ...

1989-07-01

305

Johnson News - NASA

Ion channels, transporters, and pumps as targets for heart failure

Nov 21, 2003... for the European Space Agency's Automated Transport Vehicle, a new, uncrewed station cargo vehicle targeted for launch late next year. ...

306

Imaging immune response in vivo

Congestive heart failure (CHF) is a leading cause of morbidity and mortality. CHF is marked by atrial and ventricular enlargement and reduced cardiac contractility, as well as an association...Full Text Available

2009-10-01

307

Heat shock proteins as emerging therapeutic targets

PurposeClinical trials have commenced to evaluate the feasibility of targeting malignant gliomas with genetically engineered cytolytic T-cells (CTLs) delivered directly...Full Text Available

2008-06-15

308

Genomics of human longevity

Chaperones (stress proteins) are essential proteins to help the formation and maintenance of the proper conformation of other proteins and to promote cell survival after a large variety of environmental...Full Text Available

2005-11-01

309

Gene therapy for ocular diseases

In animal models, single-gene mutations in genes involved in insulin/IGF and target of rapamycin signalling pathways extend lifespan to a considerable extent. The genetic, genomic and epigenetic influences...Full Text Available

2011-01-12

310

GAVA: Spectral Simulation for In Vivo MRS Applications

The eye is an easily accessible, highly compartmentalised and immune-privileged organ that offers unique advantages as a gene therapy target. Significant advancements have been made in understanding...Full Text Available

2011-05-01

311

Evaluation of phenylpiperazines as targeting agents for neuroblastoma.

An application that provides a flexible and easy to use interface to the GAMMA spectral simulation package is described that is targeted at investigations using in vivo MR spectroscopic methods....Full Text Available

2007-04-01

312

Effects of voicing in the recognition of concurrent syllables (L)a)

The potential of radiolabelled phenylpiperazines as agents for the detection and therapy of tumours of neural crest origin was evaluated by in vitro pharmacological studies with human neuroblastoma...Full Text Available

1996-09-01

313

Dynamic clamp with StdpC software

This letter reports a study designed to measure the benefits of voicing in the recognition of concurrent syllables. The target and distracter syllables were either voiced or whispered, producing...Full Text Available

2009-12-01

314

Dynamic Weapon-Target Assignment Problems with ...

Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based...Full Text Available

2011-03-01

315

Cooperative Charging Effects of Fibers From Electrospinning ...

... defense's weapons are Space-based kinetic-kill vehicles ... I ti I obtained by dividing the weapons as ... of the optimal weapon-arge assigntments and ...

1988-06-01

316

Comparative genomics of insect juvenile hormone biosynthesis?

... In the Proceedings of INTC 2003, we studied charging effects on target coverage during electrospinning (16). ... of Fibers from Electrospinning of ...

2005-01-05

317

Cholinergic modulation of multivesicular release regulates striatal synaptic potency and integration

The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular...Full Text Available

2006-04-01

318

Bacterial flora-typing with targeted, chip-based Pyrosequencing

The pleiotropic actions of neuromodulators on pre- and postsynaptic targets present challenges to disentangling the mechanisms underlying regulation of synaptic transmission. Within the striatum,...Full Text Available

2009-09-01

319

Approaches to the evaluation of chemical-induced immunotoxicity.

BackgroundThe metagenomic analysis of microbial communities holds the potential to improve our understanding of the role of microbes in clinical conditions. Recent, dramatic improvements...Full Text Available

320

An Extended Kalman Filter for Use in a Shared Aperture ...

The immune system plays a crucial role in maintaining health; however, accumulating evidence indicates that this system can be the target for immunotoxic effects caused by a variety of chemicals including...Full Text Available

1995-12-01

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321

A comprehensive assessment of N-terminal signal peptides prediction methods

... used to track a point source target ... A one sigma tracking error of .2 ... LOOKING INFRARED SYSTEMS, *KALMAN FILTERING, SIGNAL PROCESSING ...

1978-12-01

322

narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.

BackgroundAmino-terminal signal peptides (SPs) are short regions that guide the targeting of secretory proteins to the correct subcellular compartments in the cell. They are cleaved...Full Text Available

323

Worlds within worlds: evolution of the vertebrate gut microbiota

In previous studies it has been established that in Escherichia coli the three known subunits of anaerobic nitrate reductase are encoded by the narGHI operon. From the nucleotide sequence of the narI...Full Text Available

1988-04-01

324

Whole-genome shotgun assembly and comparison of human genome assemblies

Here we use published 16S rRNA gene sequences to compare the bacterial assemblages associated with humans, other mammals, other metazoa, and free-living microbial communities spanning a range...Full Text Available

2008-10-01

325

Whole-genome cancer analysis as an approach to deeper understanding of tumour biology

We report a whole-genome shotgun assembly (called WGSA) of the human genome generated at Celera in 2001. The Celera-generated shotgun data set consisted of 27 million sequencing reads organized in pairs...Full Text Available

2004-02-17

326

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes

Recent advances in DNA sequencing technology are providing unprecedented opportunities for comprehensive analysis of cancer genomes, exomes, transcriptomes, as well as epigenomic components. The integration...Full Text Available

2010-01-19

327

Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

328

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

The Ty transposable elements of Saccharomyces cerevisiae form a heterogeneous family within which two broad structural classes (I and II) exist. The two classes differ by two large substitutions and...Full Text Available

1985-06-11

329

Unifying evolutionary and thermodynamic information for RNA folding of multiple alignments

The diagnosis of viral causes of many infectious diseases is difficult due to the inherent sequence diversity of viruses as well as the ongoing emergence of novel viral pathogens, such as SARS coronavirus...Full Text Available

330

Two amyloid states of the prion protein display significantly different folding patterns

Computational methods for determining the secondary structure of RNA sequences from given alignments are currently either based on thermodynamic folding, compensatory base pair substitutions or both....Full Text Available

2008-11-01

331

Transposon-based screens for cancer gene discovery in mouse models

SummaryIt has been well established that a single amino acid sequence can give rise to several conformationally distinct amyloid states. The extent to which amyloid structures...Full Text Available

2010-07-23

332

Transient Hoogsteen Base Pairs in Canonical Duplex DNA

Significant emphasis has recently been placed on the characterization of the human cancer genome. This effort has been assisted by the development of new DNA sequencing technologies that allow...Full Text Available

2010-08-01

333

Transcriptome Profiling of a Toxic Dinoflagellate Reveals a Gene-Rich Protist and a Potential Impact on Gene Expression Due to Bacterial Presence

Sequence-directed variations in the canonical DNA double helix structure that retain Watson-Crick base-pairing play important roles in DNA recognition, topology, and nucleosome positioning....Full Text Available

2011-02-24

334

Transcriptional mapping of the 3' end of the bovine syncytial virus genome.

BackgroundDinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear...Full Text Available

335

The use of EPID-measured leaf sequence files for IMRT dose reconstruction in adaptive radiation therapy

The bovine syncytial virus, a member of the retroviral subfamily Spumavirinae, causes a persistent, asymptomatic infection in cattle. Nucleotide sequence analysis of the viral genome revealed two overlapping...Full Text Available

1994-02-01

336

The missing graphical user interface for genomics

For intensity modulated radiation treatment (IMRT) dose reconstruction, multileaf collimator (MLC) log files have been shown applicable for deriving delivered fluence maps. However, MLC log files are...Full Text Available

2008-11-01

337

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.

AbstractThe Galaxy package empowers regular users to perform rich DNA sequence analysis through a much-needed and user-friendly graphical web interface.See research article...Full Text Available

2010-01-01

338

The Specificity of Innate Immune Responses Is Enforced by Repression of Interferon Response Elements by NF-?B p50

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

1983-10-25

339

The LW blood group glycoprotein is homologous to intercellular adhesion molecules.

The specific binding of transcription factors to cognate sequence elements is thought to be critical for the generation of specific gene expression programs. Members of the nuclear factor κB...Full Text Available

340

The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

The LW blood group antigens reside on a 42-kDa erythrocyte membrane glycoprotein that was purified by immunoaffinity and partially sequenced. From this information, a specific PCR-amplified DNA fragment...Full Text Available

1994-06-07

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

The Application of High Resolution Sequence Stratigraphy for Understanding the Hydrocarbon Prospectivity of the Moere and Southern Voering Basins

BackgroundFood supply from the ocean is constrained by the shortage of domesticated and selected fish. Development of genomic models of economically important fishes should assist...Full Text Available

342

Ternary Complex Formation on the Adenovirus Packaging Sequence by the IVa2 and L4 22-Kilodalton Proteins?

The deep water parts of the Moere and Southern Voering Basins are large frontier areas, which are considered to contain significant undiscovered hydrocarbon resources, within Cretaceous and Paleogene reservoirs. PGS Reservoir AS have evaluated the Cretaceous and Paleogene successions of the shallow and deep water areas offshore Mid-Norway using high-resolution sequence stratigraphic techniques. The successions have been subdivided into about 20 stratigraphic sequences. The results are: (1) A more acurate and higher resolution stratigraphy, (2) Greater constraints on basin palaeoenvironmental and palaeogeographic reconstructions, (4) Fully integrated sandstone fairway models, with increased ability to reservoir and seal quality and continuity, (5) In addition to several obvious giant domal traps, subtler yet significant structural and stratigraphic leads and prospects can be identified, (6) The timing, volume and phase of hydrocarbon generation ...

1999-07-01

343

Subject-specific, multiscale simulation of electrophysiology: a software pipeline for image-based models and application examples

Assembly of infectious adenovirus particles requires seven functionally redundant elements at the left end of the genome, termed A repeats, that direct packaging of the DNA. Previous studies revealed...Full Text Available

2007-11-01

344

Studying bacterial transcriptomes using RNA-seq

Many simulation studies in biomedicine are based on a similar sequence of processing steps, starting from images and running through geometric model generation, assignment of tissue properties, numerical...Full Text Available

2009-06-13

345

Stretched DNA Investigated Using Molecular-Dynamics and Quantum-Mechanical Calculations

Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms. RNA-seq has a number of advantages over hybridization-based techniques,...Full Text Available

2010-10-01

346

Strategies for the uses of lanthanide NMR shift probes in the determination of protein structure in solutio. Application to the EF calcium binding site of carp parvalbumin.

AbstractWe combined atomistic molecular-dynamics simulations with quantum-mechanical calculations to investigate the sequence dependence of the stretching behavior of duplex DNA. Our...Full Text Available

2010-01-06

347

Straightforward and de Novo Peptide Sequencing by MALDI-MS/MS Using a Lys-N Metalloendopeptidase*S?

The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available

1980-10-01

348

Statistical properties of nucleotide clusters in DNA sequences*

In this work, we explore the potential of the metalloendopeptidase Lys-N for MALDI-MS/MS proteomics applications. Initially we digested a HEK293 cellular lysate with Lys-N and, for comparison, in parallel...Full Text Available

2009-04-01

349

Sequence variants of the DFNB31 gene among Usher syndrome patients of diverse origin

Using the complete genome of Plasmodium falciparum 3D7 which has 14 chromosomes as an example, we have examined the distribution functions for the amount of C or G and A or T consecutively...Full Text Available

2005-05-01

350

Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates

PurposeIt has been demonstrated that mutations in deafness, autosomal recessive 31 (DFNB31), the gene encoding whirlin, is responsible for nonsyndromic hearing loss...Full Text Available

351

Sequence Reactivation in the Hippocampus is Impaired in Aged Rats

alpha-actinins are actin microfilament crosslinking proteins. Vertebrate actinins fall into two classes: the broadly-expressed actinins 1 and 4 (actn1 and actn4)...Full Text Available

2009-11-01

352

Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli?

The hippocampus is thought to coordinate memory consolidation by reactivating traces from behavioral experience when the brain is not actively processing new input. In fact, during slow-wave...Full Text Available

2008-07-30

353

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis....Full Text Available

2010-05-01

354

Role of Conserved Salt Bridges in Homeodomain Stability and DNA Binding*

A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction...Full Text Available

1989-02-01

355

Remobilization of Tol2 transposons in Xenopus tropicalis

The sequence information available for homeodomains reveals that salt bridges connecting pairs 19/30, 31/42, and 17/52 are frequent, whereas aliphatic residues at these sites are rare and mainly restricted...Full Text Available

2009-08-28

356

Relationship between mRNA secondary structure and sequence variability in Chloroplast genes: possible life history implications

BackgroundThe Class II DNA transposons are mobile genetic elements that move DNA sequence from one position in the genome to another. We have previously demonstrated that the naturally...Full Text Available

357

Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae.

BackgroundSynonymous sites are freer to vary because of redundancy in genetic code. Messenger RNA secondary structure restricts this freedom, as revealed by previous findings in...Full Text Available

358

Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups

The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile...Full Text Available

1993-08-01

359

Red tides in the Gulf of Mexico: Where, when, and why?

The evolution of the human mitochondrial genome is characterized by the emergence of ethnically distinct lineages or haplogroups. Nine European, seven Asian (including Native American), and three African...Full Text Available

2002-05-01

360

Rational design of DNA sequences for nanotechnology, microarrays and molecular computers using Eulerian graphs

[1] Independent data from the Gulf of Mexico are used to develop and test the hypothesis that the same sequence of physical and ecological events each year allows the toxic dinoflagellate Karenia...Full Text Available

2006-11-07

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Rapid technique of DNA-DNA in situ hybridisation on formalin fixed tissue sections using microwave irradiation.

Nucleic acids are molecules of choice for both established and emerging nanoscale technologies. These technologies benefit from large functional densities of ‘DNA processing elements’...Full Text Available

2004-01-01

362

Rab protein evolution and the history of the eukaryotic endomembrane system

The relative sensitivities of different protocols for detecting cytomegalovirus nucleic acid sequences in histological specimens, using a biotinylated cDNA probe, were assessed. Several commonly used...Full Text Available

1987-08-01

363

Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia1

Spectacular increases in the quantity of sequence data genome have facilitated major advances in eukaryotic comparative genomics. By exploiting homology with classical model organisms, this makes possible...Full Text Available

2010-10-01

364

Primary and secondary structure of the pore-forming peptide of pathogenic Entamoeba histolytica.

Petal senescence, one type of programmed cell death (PCD) in plants, is a genetically controlled sequence of events comprising its final developmental stage. We characterized the pollination-induced...Full Text Available

2000-04-01

365

Prediction method abstracts

A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was isolated....Full Text Available

1992-10-01

366

Population genetic analysis of large sequence polymorphisms in Plasmodium falciparum blood-stage antigens

This conference was held December 4--8, 1994 in Asilomar, California. The purpose of this meeting was to provide a forum for exchange of state-of-the-art information concerning the prediction of protein structure. Attention if focused on the following: comparative modeling; sequence to fold assignment; and ab initio folding.

1994-12-31

367

Phylogeny of Mitochondrial DNA Macrohaplogroup N in India, Based on Complete Sequencing: Implications for the Peopling of South Asia

Plasmodium falciparum, the causative agent of human malaria, invades host erythrocytes using several proteins on the surface of the invasive merozoite, which have been proposed...Full Text Available

2010-03-01

368

Phylogenetic Network for European mtDNA

To resolve the phylogeny of the autochthonous mitochondrial DNA (mtDNA) haplogroups of India and determine the relationship between the Indian and western Eurasian mtDNA pools more precisely, a diverse...Full Text Available

2004-12-01

369

Photocatalytic probing of DNA sequence by using TiO{sub 2}/dopamine-DNA triads.

The sequence in the first hypervariable segment (HVS-I) of the control region has been used as a source of evolutionary information in most phylogenetic analyses of mtDNA. Population genetic inference...Full Text Available

2001-06-01

370

Phosphoglycerate kinase and triosephosphate isomerase from the hyperthermophilic bacterium Thermotoga maritima form a covalent bifunctional enzyme complex.

A method to control charge transfer reaction in DNA using hybrid nanometer-sized TiO{sub 2} nanoparticles was developed. In this system extended charge separation reflects the sequence of DNA and was measured using metallic silver deposition or by photocurrent response. Light-induced extended charge separation in these systems was found to be dependent on the DNA-bridge length and sequence. The yield of photocatalytic deposition of silver was studied in systems having GG accepting sites imbedded in AT runs at varying distances from the TiO{sub 2} nanoparticle surface. Weak distance dependence of charge separation indicative of a hole hopping through mediating adenine (A) sites was found. The quantum yield of silver deposition in the system having a GG accepting site placed 8.5 {angstrom} from the nanoparticle surface was found to be {Phi} = 0.70 (70%) and {Phi} = 0.56 (56%) for (A){sub n} and (AT){sub n/2} bridge, respectively. Hole injection ...

2007-10-15

371

Perspective on post-menopausal osteoporosis: establishing an interdisciplinary understanding of the sequence of events from the molecular level to whole bone fractures

Phosphoglycerate kinase (PGK) from the hyperthermophilic bacterium Thermotoga maritima has been purified to homogeneity. A second larger enzyme with PGK activity and identical N-terminal sequence was...Full Text Available

1995-02-01

372

Personalized Epigenomic Signatures That Are Stable Over Time and Covary with Body Mass Index

Current drug treatments for post-menopausal osteoporosis cannot eliminate bone fractures, possibly because the mechanisms responsible for bone loss are not fully understood. Although research within...Full Text Available

2010-03-06

373

Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization

The epigenome consists of non–sequence-based modifications, such as DNA methylation, that are heritable during cell division and that may affect normal phenotypes and predisposition...Full Text Available

2010-09-15

374

Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

The retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5′ ends by a dimer linkage structure. Previously it was shown that human foamy virus (HFV)...Full Text Available

2001-04-01

375

Mutational analysis of bacteriophage lambda lysis gene S.

BackgroundThe construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections...Full Text Available

376

Molecular characterization of a Chinese variant of the Flury-LEP strain

A plasmid carrying the bacteriophage lambda lysis genes under lac control was subjected to hydroxylamine mutagenesis, and mutations eliminating the host lethality of the S gene were selected. DNA sequence...Full Text Available

1986-09-01

377

Molecular Variability of Pseudallescheria boydii, a Neurotropic Opportunist

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was...Full Text Available

378

Molecular DNA Systematic Analyses of East Asian Mammals: Sequence Variation of Cytochrome b Gene and Control Region of Mitochondrial DNA of Common Otter, Lutra lutra lutra L. (Mammalia, Carnivora) from Korea

The sequences of the internal transcribed spacer (ITS) ribosomal DNA (rDNA) domain data obtained by restriction fragment length polymorphism analysis with 18S rDNA and fingerprinting (M13) for clinical...Full Text Available

2000-09-01

379

Methanogen Diversity Evidenced by Molecular Characterization of Methyl Coenzyme M Reductase A (mcrA) Genes in Hydrothermal Sediments of the Guaymas Basin

Full Text Available

2004-09-01

380

MRI features of epidural extramedullary hematopoiesis

The methanogenic community in hydrothermally active sediments of Guaymas Basin (Gulf of California, Mexico) was analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase...Full Text Available

2005-08-01

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381

Localized mutagenesis of the tetracycline promoter region in pBR322 by 4,5',8-trimethylpsoralen

A case of {beta}-thalassemia intermedia with spinal cord compression due to extramedullary hematopoiesis, which was successfully treated by blood transfusion, is presented. Emphasis was made on the MRI appearance of extramedullary hematopoiesis on different pulse sequences. The theories that aimed to explain the involvement of the epidural space by extramedullary hematopoiesis are discussed.

2000-07-01

382

Light-Dependent Regulation of Cyanobacterial Phytochrome Expression

In vitro mutagenesis of functional DNA gene fragments by covalently reactive agents permits one in principle to examine the consequent alterations in DNA sequence directly. I have carried out selective mutagenesis of the tetracycline resistance gene in the plasmid pBR322 using the long wavelength UV light activated reaction of 4,5',8-trimethylpsoralen (TMP). The mutagenized DNA sequence was the EcoR1-Hind III restriction fragment in the vicinity of the Tcsup(R) promoter. Two classes of mutants were obtained. One exhibited a high level of Tc resistance (40-60 ..mu..g/ml) but still lower than the wild-type. Interestingly, these showed no sequence alterations at all in the vicinity of the TMP-reacted fragment. The other class of mutants exhibited low levels of drug resistance (< 20 ..mu..g/ml) and two of those that were sequenced were found to contain a 15-base pair insertion to the right of ...

1982-04-01

383

Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

A histidine kinase protein (Cph1) with sequence homology and spectral characteristics very similar to those of the plant phytochrome has been recently identified in the cyanobacterium Synechocystis...Full Text Available

2000-01-01

384

Investigation of xenotropic murine leukemia virus-related virus (XMRV) in human and other cell lines.

The isolation of an untriakontapeptide from camel pituitary extracts has been described. Its structure has been determined and shown to be identical to the sequence of carboxyl-terminal 31 amino acids...Full Text Available

1976-04-01

385

Interactions in multiple schedules: negative induction with squirrel monkeys1

Xenotropic murine leukemia virus-related virus (XMRV) was discovered in human prostate tumors and later in some chronic fatigue syndrome (CFS) patients. However, subsequent studies have identified various sources of potential contamination with XMRV and other murine leukemia virus (MLV)-related sequences in test samples. Biological and nucleotide sequence analysis indicates that XMRV is distinct from known xenotropic MLVs and has a broad host range and cell tropism including human cells. Therefore, it is prudent to minimize the risk of human exposure to infection by evaluating XMRV contamination in cell lines handled in laboratory research and particularly those used in the manufacture of biological products. Nested DNA PCR assays were optimized for investigating XMRV gag and env sequences in various cell lines, which included MRC-5, Vero, HEK-293, MDCK, HeLa, and A549, that may be used in the development of some vaccines ...

2011-10-11

386

Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence

In Experiment I, lever pressing by squirrel monkeys was maintained under a sequence of variable-interval, multiple variable-interval variable-interval, and multiple variable-interval extinction schedules...Full Text Available

1978-11-01

387

Inferring transcription factor complexes from ChIP-seq data

The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically...Full Text Available

2005-11-01

388

Inferring Stabilizing Mutations from Protein Phylogenies: Application to Influenza Hemagglutinin

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) allows researchers to determine the genome-wide binding locations of individual transcription factors (TFs) at high resolution....Full Text Available

2011-08-01

389

Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation

One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions. We present...Full Text Available

2009-04-01

390

Identification of novel DNA repair proteins via primary sequence, secondary structure, and homology

The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women...Full Text Available

2003-01-01

391

Ia-antigen-T-cell interactions for a thymus-independent antigen composed of D amino acids.

BackgroundDNA repair is the general term for the collection of critical mechanisms which repair many forms of DNA damage such as methylation or ionizing radiation. DNA repair has...Full Text Available

392

IRIS Simplified LERF Model

Synthetic polypeptide antigens of L amino acids, although bearing repeating sequences, are thymus-dependent (L-TD), whereas the same polymers composed of D amino acids are thymus-independent (D-TI),...Full Text Available

1993-02-01

393

Human spumaretrovirus-related sequences in the DNA of leukocytes from patients with Graves disease.

Westinghouse is currently conducting the pre-application licensing of the International Reactor Innovative and Secure (IRIS). One of the key aspects of the IRIS design is its safety-by-designTM philosophy and within this framework the PRA is being used as an integral part of the design process. The most ambitious risk-related goal for IRIS is to reduce the Emergency Planning Zone (EPZ) to within the exclusion area by demonstrating that the off-site doses are consistent with the US Protective Action Guidelines (PAGs) for initiation of emergency response so that the required protective actions would be limited to the exclusion area. As a first step, a model has been developed to provide a first order approximation of the Large Early Release Frequency (LERF) as a surrogate predictor of the off-site doses. A key-aspect of the LERF model development is the characterization of the possible paths of release. Four main categories have been historically pointed out: (1) Core Damage (CD ) ...

2004-10-06

394

Human Pol II promoter recognition based on primary sequences and free energy of dinucleotides

Viruses, and more particularly retroviruses, have been postulated to play a role in the pathogenesis of autoimmune diseases. In a search for spumaretrovirus infection markers, we screened a group of...Full Text Available

1992-11-01

395

High-resolution sequence stratigraphic character and sandstone-type uranium ore formation. A case from Saihan Formation in Baiyinwula area, Erlian Basin

BackgroundPromoter region plays an important role in determining where the transcription of a particular gene should be initiated. Computational prediction of eukaryotic Pol II promoter...Full Text Available

396

Heterogeneous mutations in the human lipoprotein lipase gene in patients with familial lipoprotein lipase deficiency.

High-resolution sequence stratigraphy has been applied widely in the petroleum exploration and development, many achievements have been achieved. However, it is in the beginning stage that high-resolution sequence stratigraphy is applied to explore the sandstone-type uranium deposits in Erlian Basin. By applying principles of high-resolution sequence stratigraphy and taking typical boreholes as an example, sedimentary cycles of Saihan Formation, the ore-bearing formation in Baiyinwula area are divided and correlated through cross sections. One long-term cycle (LSC_1), two middle-term cycles (MSC_1, MSC_2) have been identified in this study. Based on this and combined with the mineralization character of sandstone uranium deposits in this area, it is presented that the interlayer oxidation zone is developed mainly in the rising hemicycle of MSC_1 and uranium ore bodies predominantly in channel sand bodies that were developed ...

2006-12-01

397

Genomic cloning and characterization of a ricin gene from Ricinus communis.

The DNA sequences were determined for the lipoprotein lipase (LPL) gene from five unrelated Japanese patients with familial LPL deficiency. The results demonstrated that all five patients are homozygotes...Full Text Available

1991-12-01

398

Genomic Diversity and Evolution of Mycobacterium ulcerans Revealed by Next-Generation Sequencing

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

399

Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)

Mycobacterium ulcerans is the causative agent of Buruli ulcer, the third most common mycobacterial disease after tuberculosis and leprosy. It is an emerging infectious disease that...Full Text Available

2009-09-01

400

Genome Sequence of Campylobacter jejuni strain 327, a strain isolated from a turkey slaughterhouse

BackgroundCucumber, Cucumis sativus L. is an important vegetable crop worldwide. Until very recently, cucumber genetic and genomic resources, especially molecular...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

Campylobacter is one of the leading causes of food-borne gastroenteritis and has a high prevalence in poultry. Campylobacter jejuni subsp. jejuni 327 is a subspecies...Full Text Available

402

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

BackgroundA strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased...Full Text Available

403

Full genome gene expression analysis of the heat stress response in Drosophila melanogaster

There are many methods for introducing random mutations into nucleic acid sequences. Previously, we described a suite of programmes for estimating the completeness and diversity of randomized DNA libraries...Full Text Available

2008-07-01

404

Fragment condensation of peptides on teflon with radiationally grafted polystyrene

The availability of full genome sequences has allowed the construction of microarrays, with which screening of the full genome for changes in gene expression is possible. This method can provide a wealth...Full Text Available

2005-10-01

405

Fragment condensation of peptides on teflon with radiationally grafted polystyrene

In the synthesis of the peptidyl-polymer corresponding to the sequence (57-100) of the ..beta.. chain of human hemoglobin on Teflon with radiationally grafted polystyrene a comparative study was made of different methods of fragment condensation. It was shown that the most effective methods are azide condensation and condensation with the use of complex F.

1986-12-10

406

First report of the complete sequence of Sida golden yellow vein virus from Jamaica.

In the synthesis of the peptidyl-polymer corresponding to the sequence (57-100) of the #beta# chain of human hemoglobin on Teflon with radiationally grafted polystyrene a comparative study was made of different methods of fragment condensation. It was shown that the most effective methods are azide condensation and condensation with the use of complex F.

407

Exome sequencing identifies GRIN2A as frequently mutated in melanoma

Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, ...

2011-05-29

408

Evidence for nonrandom hydrophobicity structures in protein chains.

The incidence of melanoma is increasing more than any other cancer, and knowledge of its genetic alterations is limited. To systematically analyze such alterations, we performed whole-exome...Full Text Available

2011-05-01

409

Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host

The question of whether proteins originate from random sequences of amino acids is addressed. A statistical analysis is performed in terms of blocked and random walk values formed by binary hydrophobic...Full Text Available

1996-09-03

410

Efficient discovery of ASCL1 regulatory sequences through transgene pooling

BackgroundEscherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence...Full Text Available

411

Detection of genetic mutations in repetitive DNA sequences in somatic human cells after low-dose irradiation; Nachweis genetischer Veraenderungen in repetitiven DNA-Sequenzen in somatischen menschlichen Zellen nach Bestrahlung im niedrigen Dosisbereich

Zebrafish transgenesis is a powerful and increasingly common strategy to assay vertebrate transcriptional regulatory control. Several challenges remain, however, to the broader application of...Full Text Available

2010-06-01

412

Detecting Retroviral Sequences in Chronic Fatigue Syndrome

No abstract prepared

1998-03-01

413

DNA Display I. Sequence-Encoded Routing of DNA Populations

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs....Full Text Available

414

Complexity of the microRNA repertoire revealed by next-generation sequencing

Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited...Full Text Available

2004-07-01

415

Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene.

MicroRNAs (miRNAs) have been implicated to play key roles in normal physiological functions, and altered expression of specific miRNAs has been associated with a number of diseases. It is of great interest...Full Text Available

2010-11-01

416

Complete genome sequence of Actinosynnema mirum type strain (101T)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an evolutionarily conserved glycolytic enzyme, is constitutively expressed in most cell types yet is induced to high levels during the development of...Full Text Available

1985-03-01

417

Complete characterization of the edited transcriptome of the mitochondrion of Physarum polycephalum using deep sequencing of RNA

Actinosynnema mirum Hasegawa et al. 1978 is the type species of the genus, and is of phylogenetic interest because of its central phylogenetic location in the Actino-synnemataceae, a rapidly growing family within the actinobacterial suborder Pseudo-nocardineae. A. mirum is characterized by its motile spores borne on synnemata and as a producer of nocardicin antibiotics. It is capable of growing aerobically and under a moderate CO2 atmosphere. The strain is a Gram-positive, aerial and substrate mycelium producing bacterium, originally isolated from a grass blade collected from the Raritan River, New Jersey. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first complete genome sequence of a member of the family Actinosynnemataceae, and only the second sequence from the actinobacterial suborder Pseudonocardineae. The 8,248,144 bp long single replicon genome ...

2009-05-20

418

Complete Sequencing of pNDM-HK Encoding NDM-1 Carbapenemase from a Multidrug-Resistant Escherichia coli Strain Isolated in Hong Kong

RNAs transcribed from the mitochondrial genome of Physarum polycephalum are heavily edited. The most prevalent editing event is the insertion of single Cs, with Us and dinucleotides...Full Text Available

2011-08-01

419

Coat protein gene duplication in a filamentous RNA virus of plants.

BackgroundThe emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to...Full Text Available

420

Clostridium perfringens Delta Toxin Is Sequence Related to Beta Toxin, NetB, and Staphylococcus Pore-Forming Toxins, but Shows Functional Differences

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

1992-10-01

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421

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

Clostridium perfringens produces numerous toxins, which are responsible for severe diseases in man and animals. Delta toxin is one of the three hemolysins released by a number of C....Full Text Available

422

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

423

Cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis.

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in ...

1988-10-01

424

Cloning and Sequencing of a Novel meta-Cleavage Dioxygenase Gene Whose Product Is Involved in Degradation of ?-Hexachlorocyclohexane in Sphingomonas paucimobilis

In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted by two steps of dehydrochlorination to a chemically unstable intermediate, 1,3,4,6-tetrachloro-1,4-cyclohexadiene...Full Text Available

1993-10-01

425

Cloning and Sequencing of a 2,5-Dichlorohydroquinone Reductive Dehalogenase Gene Whose Product Is Involved in Degradation of ?-Hexachlorocyclohexane by Sphingomonas paucimobilis

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide,...Full Text Available

1999-11-01

426

Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide,...Full Text Available

1998-03-01

427

Chromosome location of Oryza sativa recombination linkage groups.

In methicillin-resistant Staphylococcus aureus, the methicillin resistance gene mecA is localized within a large chromosomal region which is absent in the methicillin-susceptible...Full Text Available

1999-06-01

428

Chiropteran types I and II interferon genes inferred from genome sequencing traces by a statistical gene-family assembler

In situ hybridization, a powerful tool for the molecular cytogeneticist, can be used to physically map repetitive, low-copy, and unique DNA sequences in plant chromosomes. With the availability of a...Full Text Available

1992-09-15

429

Chemiosmotic model for plant mitochondria

BackgroundThe rate of emergence of human pathogens is steadily increasing; most of these novel agents originate in wildlife. Bats, remarkably, are the natural reservoirs of many...Full Text Available

430

Characterization of the H(+)-pumping F1F0 ATPase of Vibrio alginolyticus.

A balanced application of chemiosmosis to plant mitochondria is presented. Electron transport carriers are sequenced to achieve proton translocation across the inner membrane. The processes of proton translocation, use, and release are integrated to yield a description of the effective proton gradient available for metabolite transport and ATP synthesis. 27 references, 6 figures.

1983-12-01

431

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

The F1F0 ATPase of Vibrio alginolyticus was cloned from a chromosomal lambda library. The unc operon, which contains the structural genes for the ATPase, was sequenced and shown to have a gene organization...Full Text Available

1990-12-01

432

CLOTU: An online pipeline for processing and clustering of 454 amplicon reads into OTUs followed by taxonomic annotation

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

433

Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencing

BackgroundThe implementation of high throughput sequencing for exploring biodiversity poses high demands on bioinformatics applications for automated data processing. Here we introduce...Full Text Available

434

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

BackgroundMimivirus, a giant dsDNA virus infecting Acanthamoeba, is the prototype of the mimiviridae family, the latest addition to the family of the nucleocytoplasmic...Full Text Available

435

Behavioral genomics of honeybee foraging and nest defense

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

436

Avian Nephritis Virus (ANV) as a New Member of the Family Astroviridae and Construction of Infectious ANV cDNA

The honeybee has been the most important insect species for study of social behavior. The recently released draft genomic sequence for the bee will accelerate honeybee behavioral genetics. Although...Full Text Available

2007-04-01

437

Applications and Experience with PCR-Based Assays to Predict Blood Group Antigens

The complete RNA genome of the avian nephritis virus (ANV) associated with acute nephritis in chickens has been molecularly cloned and sequenced. Excluding the poly(A) tail, the genome comprises 6,927...Full Text Available

2000-09-01

438

Application of quantitative sedimentology in the characterization of subsurface reservoirs and outcrop analogs

SummaryDNA-based tests are increasingly being used to predict a blood group phenotype. This is possible because genes encoding 29 of the 30 blood group systems have been cloned and sequenced,...Full Text Available

2009-01-01

439

Antigenic analysis of the second extra-cellular loop of the human beta-adrenergic receptors.

The thesis deals with methods of describing the reservoir rock in central oil and gas fields on the Norwegian continental shelf. Methods of enhancing the level of recovery are dealt with. Following topics are given: Sand body dimensions and infill sequences of stable, humid-climate delta plain channels; minipermeameter-based study of permeability trends in channel sand bodies; significance of tidal cyclicity for modelling of reservoir heterogeneities in the lower Jurassic Tilje Formation, mid-Norwegian shelf; geometry and facies of large-scale flow units in fluvial-dominated fan-delta-front sequences; quantified fluvial architecture in ephemeral stream deposits of the Esplugafreda Formation (Palaeocene), Tremp-Graus Basin, northern Spain; the Safari project - collection and storage of field analogue data for quantitative reservoir modelling; sedimentary architecture of field analogues for reservoir information (SAFARI): a case study of the ...

1994-12-31

440

An Aggregate Dynamic Stochastic Model for an Air Traffic System

Polyclonal antibodies were raised in rabbits by immunization with free peptides corresponding to positions 197-222 of the human beta 1-adrenergic receptor (beta 1 peptide) and the corresponding sequence...Full Text Available

1989-10-01

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441

Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides.

processes, we construct a stochastic dynamic model for air- craft counts in ... Also , queueing models for the arrival of aircraft at ... A queueing model has also been used to study ...... Assignment and Aircraft-Sequencing Algorithms in Terminal ...

442

Acousto-optic multiplexing and demultiplexing

Amino acids labelled with dimethylaminoazobenzenesulphonyl chloride can be separated by reversed-phase high-pressure liquid chromatography and detected in the visible region (436 nm). All 19 naturally...Full Text Available

1981-12-01

443

ARISE: American renaissance in science education

A system is claimed for multiplexing or demultiplexing pulsed laser radiation having an acousto-optical device which is electrically controlled to switch a common path of high pulse rate laser radiation between a plurality of spatially distinct paths for relatively lower pulse rate laser radiation at which the pulses are sequenced according to a predetermined time pattern. The acousto-optical element typically includes a Bragg cell which is electrically driven by a set of distinct frequencies, causing deflection of radiation passing therethrough at a predetermined set of angles whereby pulsed radiation on a single path may be distributed onto the plural separate paths or radiation on plural separate paths of time-sequenced pulses of radiation can be combined into a single path of augmented pulse rate. The control of the acousto-optical element may be provided by selectively switching the output of a plurality of fixed frequency oscillators of ...

1980-06-03

444

A structural determinant required for RNA editing

The national standards and state derivatives must be reinforced by models of curricular reform. In this paper, ARISE presents one model based on a set of principles--coherence, integration of the sciences, movement from concrete ideas to abstract ones, inquiry, connection and application, sequencing that is responsive to how people learn.

1998-09-14

445

A profusion of upstream open reading frame mechanisms in polyamine-responsive translational regulation

RNA editing by adenosine deaminases acting on RNAs (ADARs) can be both specific and non-specific, depending on the substrate. Specific editing of particular adenosines may depend on the overall sequence...Full Text Available

2011-07-01

446

A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

In many eukaryotic mRNAs one or more short ‘upstream’ open reading frames, uORFs, precede the initiator of the main coding sequence. Upstream ORFs are functionally diverse as illustrated...Full Text Available

2010-01-01

447

A new perspective on phylogeny and evolution of tetraodontiform fishes (Pisces: Acanthopterygii) based on whole mitochondrial genome sequences: Basal ecological diversification?

BackgroundGenotyping analysis using capillary DNA sequencing with fluorescently labeled primer pairs obtained by polymerase chain reaction (PCR) is widely used, but is expensive....Full Text Available

448

A new application of MRT of the lung using ultra-shot turbo spin echo sequences (UTSE)

BackgroundThe order Tetraodontiformes consists of approximately 429 species of fishes in nine families. Members of the order exhibit striking morphological diversity and radiated...Full Text Available

449

A multistep bioinformatic approach detects putative regulatory elements in gene promoters

T_2 weighted ultra-short turbo spin echo sequences were used in five individuals with variations in echo times, delayed triggering and echo intervals. To reduce movement artifacts all examinations were carried out with ECG and respiratory triggering. The sequences giving optimal image quality were then employed in 19 patients having various pulmonary abnormalities. Image resolutions, artifacts, image contrasts and diagnostic value were then judged by two observers and compared with CT. In the first study, a diastole-triggered UTSE sequence with the shortest echo proved optimal (T_E=90 ms, T_R=2-4 s, echo=9 ms, turbo factor=19). In the patient series studied, MRT was inferior to CT with regard to resolution and number of artifacts, but better in respect of contrast and diagnostic value. Using UTSE of the lung, MRT can produce images of good quality. Compared with CT, contrast is better with MRT, offering diagnostic ...

450

A microarray analysis of the rice transcriptome and its comparison to Arabidopsis

BackgroundSearching for approximate patterns in large promoter sequences frequently produces an exceedingly high numbers of results. Our aim was to exploit biological knowledge for...Full Text Available

451

A hypothetical model for the peptide binding domain of hsp70 based on the peptide binding domain of HLA.

Arabidopsis and rice are the only two model plants whose finished phase genome sequence has been completed. Here we report the construction of an oligomer microarray based on the presently...Full Text Available

2005-09-01

452

A Marker-Dense Physical Map of the Bradyrhizobium japonicum Genome

The sequences of the peptide binding domains of 33 70 kd heat shock proteins (hsp70) have been aligned and a consensus secondary structure has been deduced. Individual members showed no significant...Full Text Available

1991-05-01

453

A DNA transposon-based approach to validate oncogenic mutations in the mouse

Bacterial artificial chromosome (BAC) clones are effective mapping and sequencing reagents for use with a wide variety of small and large genomes. This report describes the development of a physical...Full Text Available

2001-08-01

454

Three-frame Algorithm of Car Path Reconstruction from Airborne Traffic Data

Large-scale cancer genome projects will soon be able to sequence many cancer genomes to comprehensively identify genetic changes in human cancer. Genome-wide association studies have also identified...Full Text Available

2008-12-16

455

The nucleotide sequence of a major species of leucine tRNA from bovine liver.

The airborne traffic monitoring system forms a novel technology of detecting vehicle motion. An optical digital camera located on an airborne platform produces a series of images which then are processed to recognized the fixed vehicles. In this way the video data are converted into the time sequence of frames containing the vehicle coordinates. In the present work a three-frame algorithm is developed to identify the succeeding vehicle positions. It is based on finding the neighboring points in the frame sequence characterized by minimal acceleration. To verify and optimize the developed algorithm a ``Virtual Road'' simulator was created. Finally available empirical data are analyzed using the created algorithm.

2007-01-01

456

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

Through the use of a variety of post-labeling techniques, the nucleotide sequence of a major species of leucine tRNA from bovine liver was determined to be pG-G-U-A-G-C-G-U-G-m-G-C-ac-C-G-A-G-C-G-G-D-C-psi-A-A-G-G-C-m-G-C-U-G-G-A-psim-...Full Text Available

1980-02-25

457

Simple high-throughput annotation pipeline (SHAP)

We have isolated a genomic clone containing 'Lupinus luteus' 5S ribosomal RNA genes by screening with 5S rDNA probe clones that were hybridized previously with the initiator methionine tRNA preparation (contaminated) with traces of rRNA or its degradation products). The clone isolated contains ten repeat units of 342 bp with 119 bp fragment showing 100% homology to the 5S rRNA from yellow lupine. Sequence analysis indicates only point heterogeneities among the flanking regions of the genes. (author). 6 refs, 3 figs.

1993-01-01

458

British Library Electronic Table of Contents (United Kingdom)

Summary: SHAP (simple high-throughput annotation pipeline) is a lightweight and scalable sequence annotation pipeline capable of supporting research efforts that generate or utilize large volumes of DNA sequence data. The software provides Grid capable analysis, relational storage and Web-based full-text searching of annotation results. Implemented in Java, SHAP recognizes the limited resources of many smaller research groups. Availability: Source code is freely available under GPLv3 at External Link Not Shown. Contact: matt.demaere@unsw.edu.au; r.cavicchioli@unsw.edu.au

2011-01-01

459

Organizational demands on the man-organization interface in nuclear power plant operation

Multiplicity formulas for a class of representations of affine Kac-Moody algebras

The partial project SR 2039/4 investigates organizational demands and factors of the man-organization interface. Thee study is divided into the sections inventory of rules and guidelines; evaluation of organizational structures and modes of operation, and working out of demand characteristics and evaluation criteria. The objective consists in representing, analyzing and evaluating the efficiency structure of sequence organization, and deriving practice-relevant concept proposals in order to optimize the man-organization interface. In this regard, the working out of control mechanisms for the evaluation of organizational sequences is of considerable importance as a kind of working aid. (orig./DG).

1994-04-01

460

Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.

Simple recursion formulas are derived for the multiplicities of the dominant weight vectors appearing in a class of irreducible highest weight representations of the indecomposable affine Kac-Moody algebras. This class is characterized by the appearance of exactly two distinct infinite sequences of dominant weight vectors. The general procedure used for the enumeration of these representations and for the derivation of the corresponding multiplicity formulas is that presented by Capps for the analysis of those irreducible representations containing exactly one such infinite sequence. This procedure includes the classification of representations in terms of congruence and the identification of Weyl orbits by the norm of the dominant weight. Some of the results presented have application to physical theories such as string field theories.

1992-01-01

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461

Evolution of the primary components of massive binary stars in the case of Roche lobe overflow after main-sequence evolution

A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes. PMID:4091794

1985-12-01

462

Analysis of the tet repressor-operator interactions using the uracil-DNA glycosylase footprinting system

The components with masses 32 and 64M _s_u_n, evolved with the matter mixing in the semiconvective zone and filling their Roche lobes, after the main-sequence evolution overflow their Roche lobes and lose matter during the first part of the helium core burning. After the overflow end, the components lose the matter by stellar wind. The components shrink in the nuclear timescale. At first, they have the blue supergiant character with anomalous CNO abundance, then - the Wolf-Rayet stars character.

463

A combinatorial spanning tree model for knot Floer homology

The tet repressor regulated expression of the Tn-10-encoded tetracycline resistance determinant in a tetracycline-dependent manner. In the absence of tetracycline, the tet repressor binds as a dimer to the 19-base-pair palindromic tet operator sequence. Amino acid homologies and genetic studies with trans-dominant mutants suggest that sequence-specific recognition of the tet operator involves the extensively studied helix-turn-helix motif. We have used the uracil-DNA glycosylase (UDG) footprinting systems to identify thymine contacts in the tet operator that are essential for the formation of tet repressor-operator complexes.

1994-12-31

464

Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2.

We iterate Manolescu's unoriented skein exact triangle in knot Floer homology with coefficients in the fraction field of the group ring (Z/2Z)[Z]. The result is a spectral sequence which converges to a stabilized version of delta-graded knot Floer homology. The (E_2,d_2) page of this spectral sequence is an algorithmically computable chain complex expressed in terms of spanning trees, and we show that there are no higher differentials. This gives the first combinatorial spanning tree model for knot Floer homology.

2011-01-01

465

Making sense of theory of mind and paranoia: The psychometric properties and reasoning requirements of a false belief sequencing task.

Eight phospholipases A2 (PLAs) and four three-finger-toxins (3FTx) from the pooled venom of Bungarus fasciatus (Bf) were previously studied and sequenced, but their expression pattern in individual Bf venom and possible geographic variations remained to be investigated. We herein analyze the individual venom of two Bf specimens from Kolkata (designated as KBf) to address this question. Seven PLAs and five 3FTx were purified from the KBf venoms, and respective cDNAs were cloned from venom glands of one of the snakes. Comparison of their mass and N-terminal sequence revealed that all the PLAs were conserved in both KBf venoms, but that two of their 3FTx isoforms were variable. When comparing the sequences of these KBf-PLAs with those published, only one was found to be identical to that of Bf Vb-2, and the other five were 94-98% identical to those of Bf II, III, Va, VI and XI-2, respectively. Notably, the most abundant PLA ...

2006-12-08

466

Estimation of source term release during SGTR sequences at Wolsong plants

Introduction. This study used Item-Response Theory (IRT) to model the psychometric properties of a false belief picture sequencing task. Consistent with the mental time travel hypothesis of paranoia, we anticipated that performance on this deductive theory of mind (ToM) task would not be associated with the presence of persecutory delusions but would be related to other clinical, cognitive, and demographic factors. Method. A large (N=237) and diverse clinical and nonclinical sample differing in levels of depression and paranoid ideation performed 2 ToM tasks: the false belief sequencing task and a ToM stories task that was used to assess the validity of the false belief sequencing task as a measure of ToM. Results. A unidimensional IRT model was found to fit the data well. Latent ToM ability as measured by the false belief sequencing task was negatively related with age and positively with IQ. In ...

2011-08-15

467

Applications of high resolution sequence stratigraphy in North Sea syn-rift reservoir correlation and development

Source term release characteristics are analyzed for the severe SGTR (Steam Generator Tube Rupture) sequences beyond the design basis accidents in Wolsong 2/3/4 plants which are of CANDU6 type reactor. In PWRs, SGTR sequences have long been recognized to be important and are distinctly different from the non-bypass sequences since there is a direct fission product release path from the primary system to the environment bypassing the containment gas volume. Meanwhile, a SGTR in a CANDU reactor is analyzed not to provide a complete and direct path into the environment for the source term resulting from a severe accident. This is because the majority of the fission product released arises from heatup and interactions of the disassembled fuel channel segments and debris in the calandria tank rather than from fuel heatup in the fuel channel. These fission products are released from the calandria tank into the containment ...

1998-10-21

468

Testing of CFC Targets by Plasma Heat Fluxes Relevant to Elms and Mitigated Disruptions in ITER

Tectonically active basins may host a spectrum of sequence stratigraphic expressions previously considered to be spatially mutually exclusive. In low accommodation areas with high sediment supply, fourth order eustatic cyclicity results in high frequency sequence sets while within rapidly subsiding areas, time-equivalent Type-2 sequences are expressed by highly asymmetrical coarsening upward successions, resembling large parasequences. In the shallow marine Fulmar Formation, of the U.K. North Sea Central Graben a sequence boundary and overlying lowstand deposits, which illustrate the effects of laterally variable subsidence rate and intrabasinal topography on the expression of a eustatic sea-level fall, lie between the Glosense and Serratum (J54a and J54b) maximum flooding surfaces. The syn-rift physiography comprises major tilted fault blocks, with the Central Graben dipping parallel to the major ...

1995-08-01

469

Targeted suppression of Has2 mRNA in mouse cumulus cell-oocyte complexes by adenovirus-mediated short-hairpin RNA expression

Carbon fibre composite (CFC) was irradiated by hot plasma streams at plasma gun facility MK-200UG. The CFC targets were tested by plasma loads relevant to Edge Localized Modes (ELM) and mitigated disruptions in ITER. Onset condition of CFC evaporation and properties of evaporated carbon were studied by use of infrared pyrometry and visible spectroscopy.

2006-01-01

470

Structure functions at low Q^2: higher twists and target mass effects

RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This...Full Text Available

2009-06-01

471

Production of "2"0"3Pb by proton irradiation of Bi

We review the physics of structure functions at low Q{sup 2}, focusing on the phenomenon of quark-hadron duality and the resonance-scaling transition, both phenomenologically and in the context of quark models. We also present a new implementation of target mass corrections to nucleon structure functions which, unlike existing treatments, has the correct kinematic threshold behavior at finite Q{sup 2} in the x -> 1 limit.

2006-05-22

472

PV Conversion Technologies, Session: OPV, Sensitized, Seed (Presentation)

Production of lead-203 by proton irradiation of bismuth metal has been investigated as a replacement method for commercially available lead-203. Targets of bismuth metal (0.1 cm thick) were irradiated for periods of 1-3 hours with 90 MeV protons. After processing of the target, the estimated yield of lead-2-3 was 30 millicuries/microamp.

1990-06-24

473

Modeled Neutron Induced Nuclear Reaction Cross Sections for Radiochemistry in the region of Iridium and Gold

The NREL Sensitized Solar Cell (SSC) Core Program supports the Solar America Initiative by: (1) targeting new devices and processes for commercialization by 2015 that are less expensive, more efficient, highly reliable, and environmentally benign; (2) collaborating with DOE OS/BES to conduct basic research targeting breakthroughs in key areas, such as ultra-high efficiency and/or ultra-low cost materials and devices.

2008-04-01

474

Kidney-specific allo- and autoantibodies in the alloantibody response to rat kidney: the use of kidney homogenate as a target for serological analysis.

We have developed a set of modeled nuclear reaction cross sections for use in radiochemical diagnostics. Systematics for the input parameters required by the Hauser-Feshbach statistical model were developed and used to calculate neutron induced nuclear reaction cross sections for targets ranging from osmium (Z = 76) to gold (Z = 79). Of particular interest are the cross sections on Ir and Au including reactions on isomeric targets.

2008-02-01

475

In vitro atrazine-exposure inhibits human natural killer cell lytic granule release

LEW anti-DA kidney and DA anti-LEW kidney sera were assayed using an indirect 125I anti-immunoglobulin-binding assay with kidney homogenate as target. This allowed the full spectrum of antibodies to...Full Text Available

1980-04-01

476

Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or heteromeric artificial miRNA targeting AP1 and CAL genes

The herbicide atrazine is a known immunotoxicant and an inhibitor of human natural killer (NK) cell lytic function. The precise changes in NK cell lytic function following atrazine exposure have not been fully elucidated. The current study identifies the point at which atrazine exerts its affect on the stepwise process of human NK cell-mediated lyses of the K562 target cell line. Using intracellular staining of human peripheral blood lymphocytes, it was determined that a 24-h in vitro exposure to atrazine did not decrease the level of NK cell lytic proteins granzyme A, granzyme B or perforin. Thus, it was hypothesized that atrazine exposure was inhibiting the ability of the NK cells to bind to the target cell and subsequently inhibit the release of lytic protein from the NK cell. To test this hypothesis, flow cytometry and fluorescent microscopy were employed to analyze NK cell-target cell co-cultures following atrazine ...

2007-06-01

477

Electron accelerator unit for electron beam therapy

Gene silencing is a useful technique for elucidating biological function of genes by knocking down their expression. A recently developed artificial microRNAs (amiRNAs) exploits an endogenous...Full Text Available

2009-03-01

478

Electron accelerator unit for electron beam therapy

An electron accelerator unit is described for electron beam therapy, comprising: a source of an electron beam; means for finally directing at least a portion of the beam to a therapy site, the directing means being mechanically independent of, and electrically isolated from, the source, and having a target area; and means for aligning the source with the directing means, the aligning means comprising means for projecting at least one beam of light from the source toward the target area.

1987-01-27

479

Double-electron-capture cross section for I/sup +/ in a magnesium-vapor target

An electron accelerator unit is described for electron beam therapy, comprising: a source of an electron beam; means for finally directing at least a portion of the beam to a therapy site, the directing means being mechanically independent of, and electrically isolated from, the source, and having a target area; and means for aligning the source with the directing means, the aligning means comprising means for projecting at least one beam of light from the source toward the target area.

480

Development of High-Efficiency Low-Lift Vapor Compression System - Final Report

Measurements of the double-electron-capture process in which a positive ion of iodine becomes a negative ion in a single collision with a magnesium atom are reported between 20 and 90 keV. The cross section is comparable to that for the rare gases and not as large as might be expected from a two-valence-electron atom. This process is probably insignificant in the production of negative ion beams using a magnesium-vapor target.

1987-06-15

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481

Designing Human m₁ Muscarinic Receptor-Targeted Hydrophobic Eigenmode Matched Peptides as Functional Modulators

PNNL, with cofunding from the Bonneville Power Administration (BPA) and Building Technologies Program, conducted a research and development activity targeted at addressing the energy efficiency goals targeted in the BPA roadmap. PNNL investigated an integrated heating, ventilation and air conditioning (HVAC) system option referred to as the low-lift cooling system that potentially offers an increase in HVAC energy performance relative to ASHRAE Standard 90.1-2004.

2010-03-31

482

Achieving cholesterol targets by individualizing starting doses of statin according to baseline low-density lipoprotein cholesterol and coronary artery disease risk category: The CANadians Achieve Cholesterol Targets Fast with Atorvastatin Stratified Titration (CanACTFAST) study

A new proprietary de novo peptide design technique generated ten 15-residue peptides targeting and containing the leading nontransmembrane hydrophobic autocorrelation wavelengths, “modes”,...Full Text Available

2004-03-01

483

A bioinformatics tool for linking gene expression profiling results with public databases of microRNA target predictions

BACKGROUND:Despite an increasing body of evidence on the benefit of lowering elevated levels of low-density lipoprotein cholesterol (LDL-C), there is still considerable concern that...Full Text Available

2010-02-01

484

Radiation inactivation target size of rat adipocyte glucose transporters in the plasma membrane and intracellular pools

MicroRNAs are short (∼22 nucleotides) noncoding RNAs that regulate the stability and translation of mRNA targets. A number of computational algorithms have been developed to help predict which...Full Text Available

2008-11-01

485

Proton G{sub E}/G{sub M} from beam-target asymmetry

The in situ assembly states of the glucose transport carrier protein in the plasma membrane and in the intracellular (microsomal) storage pool of rat adipocytes were assessed by studying radiation-induced inactivation of the D-glucose-sensitive cytochalasin B binding activities. High energy radiation inactivated the glucose-sensitive cytochalasin B binding of each of these membrane preparations by reducing the total number of the binding sites without affecting the dissociation constant. The reduction in total number of binding sites was analyzed as a function of radiation dose based on target theory, from which a radiation-sensitive mass (target size) was calculated. When the plasma membranes of insulin-treated adipocytes were used, a target size of approximately 58,000 daltons was obtained. For adipocyte microsomal membranes, we obtained target sizes of approximately 112,000 and 109,000 daltons prior ...

1987-06-15

486

One-class classifiers and their application to synthetic aperture radar target recognition

The ratio of the proton's electric to magnetic form factor, G{sub E}/G{sub M}, can be extracted in elastic electron-proton scattering by measuring cross sections, beam-target asymmetry, or recoil polarization. Separate determinations of G{sub E}/G{sub M} by cross sections and recoil polarization observables disagree for Q{sup 2}>1 (GeV/c){sup 2}. Measurement by a third technique might uncover an unknown systematic error in either of the previous measurements. The beam-target asymmetry has been measured for elastic electron-proton scattering at Q{sup 2} = 1.51 (GeV/c){sup 2} for target spin orientation aligned perpendicular to the beam momentum direction. This is the largest Q{sup 2} at which G{sub E}/G{sub M} has been determined by a beam-target asymmetry experiment. The result, {mu}G{sub E}/G{sub M}=0.884{+-}0.027{+-}0.029, is compared to previous world data.

2006-09-15

487

One-class classifier networks for target recognition applications

Target recognition requires the ability to distinguish targets from non-targets, a capability called one-class generalization. To function as a one-class classifier, a neural network must have three types of generalization: within-class, between-class, and out-of-class. We discuss these three types of generalization and identify neural network architectures that meet these requirements. We have applied our one-class classifier ideas to the problem of automatic target recognition in synthetic aperture radar. We have compared three neural network algorithms: Carpenter and Grossberg`s algorithmic version of the Adaptive Resonance Theory (ART-2A), Kohonen`s Learning Vector Quantization (LVQ), and Reilly and Cooper`s Restricted Columb Energy network (RCE). The ART 2-A neural network has given the best results, with 100% within-class, and out-of-class generalization. Experiments show that the network`s ...

1992-10-01

488

Integral cross sections of 50.5 MeV #alpha# particle inelastic scattering on 1p and (2s-1d) shell nuclei and scattering mechanisms

Target recognition requires the ability to distinguish targets from non-targets, a capability called one-class generalization. Many neural network pattern classifiers fail as one-class classifiers because they use open decision boundaries. To function as one-class classifier, a neural network must have three types of generalization: within-class, between-class, and out-of-class. We discuss these three types of generalization and identify neural network architectures that meet these requirements. We have applied our one-class classifier ideas to the problem of automatic target recognition in synthetic aperture radar. We have compared three neural network algorithms: Carpenter and Grossberg`s algorithmic version of the Adaptive Resonance Theory (ART-2A), Kohonen`s Learning Vector Quantization (LVQ), and Reilly and Cooper`s Restricted Coulomb Energy network (RCE). The ART 2-A neural network gives the best ...

1993-01-01

489

Donut-shaped high-dose configuration for proton beam radiation therapy

Obtained experimental data on integral cross sections (ICS) of inelastic scattering of 50.5 MeV #alpha# particles with the excitation of "6","7Li, "9Be, "1"2","1"3C, "1"4C, "1"4N, "2"0Ne, "2"4Mg, "2"8Si nucleus low-lying energy levels are discussed. Regularities, detected in the behaviour of ICS forward scattering for 20-90 deg angles and backscattering for 90-160 deg angles for the target-nucleus under investigation are considered. Effect of reaction open channel number on #alpha#-particle scattering ICS where n,p,d- and #alpha#-channels were considered as the main channels for all the target-nuclei, is discussed. Dependence of #alpha#-particle scattering ICS on the target-nucleus level excitation energy and dependences of reaction open channel number on the channel spin, calculated for 50.5 MeV #alpha# particles and different target nuclei are shown in the diagrams. It is noted that the observed ...

490

Donut-shaped high-dose configuration for proton beam radiation therapy

Background: the authors report on the conception and first clinical application of a donut-shaped high-dose configuration for proton therapy (PT). This approach allows one to intensify target volume dose coverage for targets encompassing a critical, dose-limiting structure - like here, the cauda equina -, whilst delivering minimal dose to other healthy structures surrounding the target, thereby reducing the integral dose. Methods and results: intensity-modulated PT methods (IMPT) for spot scanning were applied to create and deliver a donut-shaped high-dose configuration with protons, allowing treating > 75% of the target with at least 95% of the prescribed dose of 72.8 CGE, whilst restricting dose to the cauda equina to 60-65 CGE. Integral dose was lower by a factor of 3.3 as compared to intensity-modulated radiotherapy with photons (IMXT). Conclusion: IMPT and spot scanning technology allow a ...

2005-01-01

491

Radioactive targets for neutron-induced cross section measurements

Background: the authors report on the conception and first clinical application of a donut-shaped high-dose configuration for proton therapy (PT). This approach allows one to intensify target volume dose coverage for targets encompassing a critical, dose-limiting structure - like here, the cauda equina -, whilst delivering minimal dose to other healthy structures surrounding the target, thereby reducing the integral dose. Methods and results: intensity-modulated PT methods (IMPT) for spot scanning were applied to create and deliver a donut-shaped high-dose configuration with protons, allowing treating > 75% of the target with at least 95% of the prescribed dose of 72.8 CGE, whilst restricting dose to the cauda equina to 60-65 CGE. Integral dose was lower by a factor of 3.3 as compared to intensity-modulated radiotherapy with photons (IMXT). Conclusion: IMPT and spot scanning technology allow a ...

2005-01-01

492

Simultaneous production of "2"8Mg and "4"7Ca by high-energy heavy-ion irradiation for applications in biology

Measurements using radioactive targets are important for the determination of key reaction path ways associated with the synthesis of the elements in nuclear astrophysics (sprocess), advanced fuel cycle initiative (transmutation of radioactive waste), and stockpile stewardship. High precision capture cross-section measurements are needed to interpret observations, predict elemental or isotopical ratios, and unobserved abundances. There are two new detector systems that are presently being commissioned at Los Alamos National Laboratory for very precise measurements of (n,{gamma}) and (n,f) cross-sections using small quantities of radioactive samples. DANCE (Detector for Advanced Neutron-Capture Experiments), a 4 {pi} gamma array made up of 160 BaF{sub 2} detectors, is designed to measure neutron capture cross-sections of unstable nuclei in the low-energy range (thermal to {approx}500 keV). The high granularity and high detection efficiency of DANCE, combined with ...

2004-01-01

493

SiO{sub 2}-Ta{sub 2}O{sub 5} sputtering yields: simulated and experimental results

With the aim of preparing carrier-free "2"8Mg and "4"7Ca simultaneously, Ti, V and Fe targets were examined by irradiating with high-energy ions of "1"2C, "1"4N and "1"6O accelerated by the RIKEN ring cyclotron. Among the targets, V gave the highest cross section for the formation of both "2"8Mg and "4"7Ca irrespective of the kind of beams. The cross section for the formation of "2"8Mg by the reactions of Ti, V and Fe targets with ion beams increased in the order of "1"2C<"1"6O<"1"4N. On the other hand, the three beams exhibited almost the same cross sections for the formation of "4"7Ca by the reaction of a given target. Titanium and V were selected as prospective targets and "1"4N as a suitable beam for the production of "2"8Mg and "4"7Ca. Chemical separation procedures of the radiotracers in carrier- and salt-free states have been established by using cation exchange resins. ...

494

Measurement of K x-ray intensity ratio of tin, gadolinium and dysprosium

To improve mirrors coating, we have modeled sputtering of binary oxide targets using TRIM code. First, we have proposed a method to calculate TRIM input parameters using on the one hand thermodynamic cycle and on the other hand Malherbe`s results. Secondly, an iterative processing has provided for oxide steady targets caused by ionic bombardment. Thirdly, we have exposed a model to get experimental sputtering yields. Fourthly, for (Ar - SiO{sub 2}) pair, we have determined that steady target is a silica one. A good agreement between simulated and experimental yields versus ion incident angle has been found. For (Ar - Ta{sub 2} O{sub 5}) pair, we have to introduce preferential sputtering concept to explain discrepancy between simulation and experiment. In this case, steady target is tantalum monoxide. For (Ar - Ta+O{sub 2}) pair, tantalum sputtered by argon ions in reactive oxygen atmosphere, we have to ...

1994-09-01

495