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Establishment of a method for RIA of transferrin

The authors discuss radioimmunoassays (RIAs) for eicosanoids widely used in the field of experimental, clinical and pharmacological research. The main drawback of RIAs is the possibility of low specificity. This was clearly demonstrated during the controversy on plasma levels of 6-keto-PGF_1 #alpha#, the stable hydrolysis product of PGI_2. When extensive purification including reverse phase high pressure liquid chromatography (rp-HPLC) was performed prior to the RIA for 6-keto-PGF_1 #alpha# the levels were at least one order of magnitude less than previously claimed, and did not support the so-called circulating hormone hypothesis.

The ROC's Maritime Claims and Practices with Special Reference to the South China Sea

Objective: To develop a method for RIA of transferrin without dilution of the serum sample. Methods: The measurable range was broadened through: Lowering of the specific radioactivity of the labelled antigen, increasing the concentration of the anti-serum and improve the curve fitting method. Results: The antiserum affinity was 3.82 x 10"-"1"0 mol/L, the specific radioactivity of the labelled antigen was 15.3 mg/mic, inter-assay error CV 5.38%, intra-assay error CV = 3.5% and the shape of the curve was good. For 30 normal control specimens, the mean TF content was 2.81 +- 0.972 mg/ml. Conclusion: Development of a method for RIA of TF without previous dilution of the test serum was of clinical value

2002-04-01

British Library Electronic Table of Contents (United Kingdom)

The South China Sea is an area of disputes on sovereignty and resource jurisdiction claims. As one of the six claimants, the Republic of China (Taiwan) has played an important role not only because it is the originator of the U-shaped lines, but also it has continuously occupied the largest island, Tai-Ping-Dao, in the Spratlys. This article reviews the ROC's position on the issues through an exploration of its maritime claims to the territorial sea, continental shelf, and exclusive economic zone.

2010-01-01

EOP-MOD Ver 2.0 2444055.5 1.0 11875 UT1-TAI UNDEF # Earth ...

Failure to detect circulating DNA--anti-DNA complexes by four radioimmunological methods in patients with systemic lupus erythematosus.

... 2.3385 -27064396 0.0037 0.0030 15. .000 .000 .000 2449038.5 1.9466 2.3213 - 27066977 0.0037 0.0035 12. .000 .000 .000 2449039.5 1.9312 2.3064 -27069688 ...

UK PubMed Central (United Kingdom)

The presence of DNA-anti-DNA complexes in sera from patients with systemic lupus erythematosus (SLE) was investigated by two new radioimmunoassays (RIA) developed for this purpose and by measuring the...Full Text Available

1977-12-01

Estimating Farm-Level Costs of Requirements Under Water Protection Zones Element of the Water Framework Directive

Environmental Research Database

DescriptionFarm-level estimates of the financial impact of regulation are important indicators of the effect that a regulation have. Such estimates should be included in RIA, particularly within the Small Firms Impact Test. This farm-level analysis can be used in the RIA to inform judgements of the affordability of the regulation proposal. It will help to identify which farm types are likely to be financially had-hit from the regulation. Farm-level cost estimates for each regulation are also required t [continued...

2007-01-23

Developing with Ext GWT Enterprise RIA Development

CERN Document Server

Developing in Ext GWT is a fast--paced, practical guide to quickly learning the tasks necessary in building enterprise--class Rich Internet Applications. Based around the exciting new UI library from Ajax leaders Ext JS and the latest GWT release, this book takes the reader through setup, the available widgets, and advanced custom widgets and templates, and concludes with a functional sample client--server application in less than 150 pages. Not your typical beginner's guide to programming, this book provides a rapid approach to becoming effective with leading commercial RIA tools and librarie

2009-01-01

Interaction of thyroid hormone and hemoglobin: nature of the interaction and effect of hemoglobin on thyroid hormone radioimmunoassay

Specificity of the human proteoglycan radioimmunoassay

Gel filtration of human erythrocyte (RBC) lysate incubated with labeled thyroxine (Tu) or triiodothyronine (Tt) revealed co-elution of a major iodothyronine-binding fraction (R-2) and hemoglobin. Solutions of purified human hemoglobin and Tt also showed co-elution of hormone and hemoglobin. Because hematin and protoporphyrin were shown to bind labeled Tt, the oxygen-binding site on hemoglobin was excluded as the site of iodothyronine-hemoglobin interaction. Analysis of hormone binding by heme and globin moieties showed Tt binding to be limited to the heme fraction. Addition of excess unlabeled Tt to hemoglobin or heme incubated with labeled Tt indicated 75% to 90% of hormone binding was poorly dissociable. These observations suggested that the presence of hemoglobin in RBC lysate or in serum could influence the measurement of Tu and Tt by specific radioimmunoassay (RIA). Subsequent studies of the addition to serum of human hemoglobin revealed a significant ...

Relationship between serum heat-stable alkaline phosphatase level and pregnancy

The human articular cartilagineous proteoglycans (PG) R.I.A. is highly specific. The PG used as the standard and the /sup 125/I labelled molecule appear to be pure. Under these conditions, all the potential interfering substances which have been tested show no cross reaction. For instance, the Ag-Ab equilibrium is not affected by adding human IgG, human albumin, hyaluronic acid, chondroitin sulfate, rat type II collagen or total human serum proteins. This R.I.A. also exhibits a species spcificity since there is no cross reaction with rat PG and negligible cross section with dog PG. The results obtained after addition of enzymes to the antigen demonstrate that the antigenic sites are localized on the protein region and not on the glycosaminoglycan region of the molecule.

1981-01-01

Heterophyle antibodies causing false positive radio-immunoassay results a case report

Serum heat-stable alkaline phosphatase (HSAP) level in 649 cases of normal pregnancy and 164 cases of high-risk pregnancy is measured by radioimmunoassay (RIA). The results indicate that the HSAP level in normal pregnancy increased proportionally with gestation weeks (r = 0.9843). In 33 cases of pregnancy induced hypertension and 21 cases of intrauterine fetal growth retardation, the HSAP level is significantly low. In 7 cases of neonatal asphyxia and 26 cases of fetal distress, the HSAP level in the mother's serum is also low. In 53 cases of intrahepatic cholestasis of pregnancy, the HSAP level is similar to those of normal pregnancy. This study illustrates that HSAP RIA can play an important role in the evaluation of placental function and fetal prognosis for cases of high-risk pregnancy.

1998-01-01

Assessment of periodic sub-diurnal Earth rotation variations at tidal frequencies through transformation of VLBI normal equation systems

A case report of falsely elevated serum hormone values measured by radio-immunoassay (RIA) is described. The radio-immunoassays concerned have a first antibody raised in rabbits and mostly a separation technique based on a second antibody-solid phase system. The presence of heterophyle (anti-rabbit) antibodies in patients' serum is proved.

British Library Electronic Table of Contents (United Kingdom)

We present an empirical model for periodic variations of diurnal and sub-diurnal Earth rotation parameters (ERPs) that was derived based on the transformation of normal equation (NEQ) systems of Very Long Baseline Interferometry (VLBI) observing sessions. NEQ systems that contain highly resolved polar motion and UT1-TAI with a temporal resolution of 15?min were generated and then transformed to the coefficients of the tidal ERP model to be solved for. To investigate the quality of this model, comparisons with empirical models from the Global Positioning System (GPS), another VLBI model and the model adopted by the conventions of the International Earth Rotation and Reference Systems Service (IERS) were performed. The absolute coefficients of these models agree almost completely within 7.5?...

2011-01-01

Analytical study on integrity of BWR reactor internal structures against water hammer under RIA conditions

Wear-resistance of manganese steel surface composites with cast tungsten carbide particles; Wc-W2C ryushi ni yori hyomen fukugokashita mangan ko no tai mamosei

The integrity of the RPV head and reactor internals was assessed by means of fluid-structural analyses using a coupled method to evaluate the water hammer phenomenon arising from high burnup fuel failure under RIA conditions. The fluid viscosity effect on the water column burst as well as the complex three-dimensional flow paths caused by a core shroud and standpipes were considered in this study. The three analysis scenarios were designed to investigate the above mentioned influential factors separately. In the first scenario, a two-dimensional axisymmetric reactor vessel model without any reactor internals was modeled to assess the influence of the fluid dynamics in the NSC RIA regulatory evaluation. This model has an actual RPV geometry and can be simply separated from other influential factors in order to concentrate only on investigation of the fluid viscosity effect. In the second scenario, a two-dimensional axisymmetric reactor vessel ...

2003-07-01

Fundamental study on evaluation method for alkali-resistance of fibers. Kakushu sen'i no tai arukarisei no hyokaho ni kansuru kiso kenkyu

To make the surface composites with hard ceramics particles is an effective means for satisfying the request for the wear-resistance of the casting products. Covering casting is a general method for forming the composites by adding the adhesive into ceramics particles. However, due to the using of adhesive, pore and intermediate are easy to generate, and it is one of the reasons making the wear-resistance unstable. In the present study, it is attempted to form the wear-resistant composite layer by dispersing cast tungsten carbide (CTC) particles on the surface of wear-resistant 7 mass% Mn steel without using adhesives. Subsequently, the experiments on the wear-resistance of the obtained surface composites under several wear conditions are carried out. It is revealed by the results thereof that 7 mass% Mn steel surface composites with CTC particles have the wear-resistance which is even more excellent than those of white cast iron and cast chromium cast iron. It is also found that the ...

1995-04-25

Effects of atmospheric pressure on tracking failure of organic insulating materials; Yuki zetsuen zairyo no tai tracking sei ni oyobosu kiatsu no eikyo

In order to clarify the alkali-resistance of fibers to be utilized as reinforcing materials for concrete, such as glass fiber, Aramid fiber, and carbon fiber, accelerated aging tests in alkaline solution were conducted. In detail, the degree of degradation of the fracture strength and Young's modulus were investigated through static tensile strength tests after soaking in alkaline solution of these fibers and then the mechanism of degradation was examined by observation using a scanning electron microscope. The conclusion obtained was as follows. Glass fiber was easily degraded in the alkaline solution and the progress of degradation was simulated quantitatively by the method proposed in this paper, which was based on a diffusion theory. Both Aramid fiber and carbon fiber showed a significant alkali-resistance without degrading. The reaction of glass fiber with alkaline solution proceeded uniformly from the surface of glass fiber into the inner direction with passage of time. ...

1994-05-20

On the Resolving Power of a Single Exact-Repeat Altimetric Satellite or a Coordinated Constellation of Satellites

The effect of atmospheric pressure on tracking resistance of organic insulating materials were investigated in the range of 500 to 1007 hPa. The tracking resistance for Polycarbonate (PC), Modified Polyphenylene Oxide (M-PPO) and Paper Base Phenolic Resin Laminate (PL) increase with the decrease in atmospheric pressure. The scintillating discharges on these samples surface lead to the ignition. The tracking failure on these results from the ignition on the surface. For Polybutylene Terephthalate (PBT) and Polyethylene Terephthalate (PET), the tracking resistance decrease with the decrease in atmospheric pressure. The discharges observed on the these surface are the glow-like. The discharge area spreads on the surface between the electrodes at the lower pressure. The failure on this group results from the carbonization by the discharges on the surface. However, the tracking resistance for Unsaturated Polyester Resins (UP) has no significant dependence on lower atmospheric pressure. 9 ...

1995-11-20

Robust curve fitting method for single binding site mass action model of radioimmunoassay

It is proved that the mid-point grid, which is composed of samples obtained at ground track locations midway between crossover points (thus a subset of the full sampling), has the same resolving power as the full set; i.e., they resolve the same three-dimensional spectral space. The resolving power of the mid-point grid as derived previously by Tai (1995, 1998) is characterized by the Nyquist frequency ? c=? /T (where T is the repeat period of the exact-repeat satellite), and by (in local Cartesian coordinates) the zonal and meridional Nyquist wavenumber kc=2? /X and lc=2? /Y respectively (where X and Y are the east-west and north-south separation between adjacent parallel ground tracks). Here we re-derive this result in simplified terms. First, it is shown through the sampling theorem as demonstrated previously by Tai (1999) that even though samples of the real mid-point grid are not taken simultaneously, they resolve the same frequency range ...

2001-12-01

Screening for congenital hypothyroidism in Lebanon

The difficulty and unstability of the parameter estimation of mass action model for radioimmunoassay are the major obstacles to its application in routine work. A unique robust curve fitting method for the mass action model was introduced. large numbers of simulated data were generated with Monte Carlo method to compare the accuracy and robustness of the robust fitting and the least squre fitting (Marquardt method) of the mass action model. The results demonstrated that the new robusst method significantly increased the accuracy and reliability of data processing with the mass action model. It also markedly reduced the influence of outliers in the fitting of RIA data, and it was always 'convergent'. This robust method could also be applied to other mathematical models which can be transformed into high degree equations and no initial estimates are needed for curve fitting.

Prostaglandin (PG) E3 synthesis elicted by adrenergic stimuli in guinea-pig trachea (GPT) is mediated primarily by B2 adrenergic receptors

Full text.National programs of neonatal screening were introduced in most countries and have proved their effectiveness against different types of retardation in children. Congenital hypothyroidism is one of the most common causes of physical and mental retardation in children. Programs of screening have shown that the incidence of this disorder is ethnic-background: it is low in Africans: 1-32000 whereas it is 1-3500 and 1-5000 in Hispanic and European newborns respectively. No previous mass screening programs were done in Lebanon. For this purpose, this program was introduced in collaboration with the IAEA. Cord blood is spotted into circles on filter paper just after delivery. These samples are air-dried at room temperature and kept at 4 degree C. They are then collected from hospitals to be screened. In the laboratory, all samples are assayed for IRMA TSH first. Those showing high or borderline TSH level (20mU/L) are then assayed for RIA TT4. Only those showing ...

1999-11-02

1 2

Prostaglandin (PG) E3 synthesis elicted by adrenergic stimuli in guinea-pig trachea (GPT) is mediated primarily by B2 adrenergic receptors

The purpose of this study was to examine arachidonic acid (AA) metabolism and to characterize the type of adrenergic receptor (AR) involved in the production of the major metabolite of this fatty acid. ({sup 14}C)AA was incubated with GPT-rings and the radiolabelled products were extracted and separated by TLC method. The medium was also assayed for radiolabelled immunoreactive PG's (iPG's) and leukotrienes (LT) B4 and C4 by RIA or Enzyme immunoassay (EIA) after exposure to various AR agonists. ({sup 14}C)AA was incorporated into GPT-rings and metabolized mainly into iPGE2 and smaller amounts into PGF2{alpha}. Trace amounts of PGD2 and 6-keto-PGF1{alpha} but not LTB4 or LTC4 were detected by RIA and/or EIA. Incubation of GPT rings for 15 minutes with isoproterenol and salbutamol resulted in a significant increase of PGE2 synthesis (optimum conc: 10{sup {minus}7}, 10{sup {minus}7}M respectively). In contrast, dobutamine, ...

1990-02-26

Radioimmunoassay determination of the effect on animal reproduction of alternative of feeding suplementation in dairy cows

The purpose of this study was to examine arachidonic acid (AA) metabolism and to characterize the type of adrenergic receptor (AR) involved in the production of the major metabolite of this fatty acid. ["1"4C]AA was incubated with GPT-rings and the radiolabelled products were extracted and separated by TLC method. The medium was also assayed for radiolabelled immunoreactive PG's (iPG's) and leukotrienes (LT) B4 and C4 by RIA or Enzyme immunoassay (EIA) after exposure to various AR agonists. ["1"4C]AA was incorporated into GPT-rings and metabolized mainly into iPGE2 and smaller amounts into PGF2#alpha#. Trace amounts of PGD2 and 6-keto-PGF1#alpha# but not LTB4 or LTC4 were detected by RIA and/or EIA. Incubation of GPT rings for 15 minutes with isoproterenol and salbutamol resulted in a significant increase of PGE2 synthesis (optimum conc: 10"-"7, 10"-"7M respectively). In contrast, dobutamine, norepinephrine, phenylnephrine and xylazine (up to ...

1991-04-21

Changes of serum leptin, adiponection and insulin levels in females with simple obesity

The principal object of this trial was to evaluate the influence of three alternatives of feeding suplementation in dairy cows in the post-partum period in ecuadorian highlands. Thirty sic animals in fist lactation were used in this experiment and were divided in three groups according to the feed intake: Group A diet was 5 Kg. of a commercial concentrate mixture with 12 per cent of crude protein plus pasture ad libitum; Group B diet was green banans (Musa paradisiaca) and pasture and Group C diet was the control only pasture. Using Radioimmunoassay technique (RIA), progesterone values were determinated in milk from each cow. the sampling was sequential, two samples a week, starting 6 days after parturition, until the animal was pregnant or until the study was finished, 150 days after post-partum for each cow. This research allowed us to evaluate the ovaric post-partum activity of each group: Frequency and length of the oestrus cycles; efficiency of oestrus ...

Serumal rate of the chromogranin in neuroendocrine tumor ET; Taux serique de la chromogranine a ET tumeurs neuroendocrines

Objective: To study the changes of serum leptin, insulin and adiponectin levels and their relationship with BMI in females with simple obesity. Methods: Serum leptin, adiponectin and insulin levels were measured with RIA in 48 pre-obese females (BMI=23-24.9 kg/m"2), 40 females with simple obesity, (BMI#>=#25 kg/m"2) and 42 female controls (BMI 18-22.9 kg/m"2). Correlations among these variables were studied. Results: Serum leptin, insulin levels were significantly higher and serum adiponectin levels were significantly lower in both the pre-obese and obese females than those in controls. Serum leptin, insulin levels were positively correlated to BMI; Serum adiponectin levels were negatively correlated to BMI. Conclusion: Within normal range of BMI, the leptin-insulin feedback mechanism provided satisfactory self-regulation. However, with excessive BMI, this dynamic equilibrium would be disrupted. The defective equilibrium, together with the abnormal low ...

2004-08-01

Immunological detection and quantification of DNA components structurally modified by alkylating carcinogens, mutagens and chemotherapeutic agents

The intracellular A chromogranin (ACg) is specific in neuroendocrine cells. This work is a contribution to the study of clinical interest of serumal dosage of this protein in a series of patients carrying neuroendocrine tumors (NET). From January `94 to November `96, 143 patients were subjected to OctreoScan scintigraphy and/or to MIBG. In 104 of them a dosage of ACg by RIA method was effectuated. A study of a statistical relation was performed on the rate of ACg and histopathological, clinical and scintigraphic criteria. Analysis of results excluded the patients with abnormal creatininemy; it referred exclusively to the patients the histopathology of whose it was proved. The global results concerning the sensitivity and specificity of ACg are: 68% and 92%, respectively, for a threshold of normality at 100 ng/ml; 65% and/or 100% for a threshold at 110 ng/ml. There is a significant difference between the group of patients with NET (70 patients): 371 {+-} 59 ng/ml ...

1997-12-31

Effect of feeding and temperature on the circadian rhythms of cortisol, thyroxine and triiodothyronine in pigs

The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 10"9 to > 10"1"0 lmol) specifically directed against several DNA alkylation products with possible relevance in relation to both mutagenesis and malignant transformation of mammalian cells. These alkylation products include ...

1983-09-01

Clinical significance of changes of plasma endothelial vasoactive factors contents after treatment in patients with systemic sclerosis (SSc)

An experiment was conducted to evaluate the circadian rhythms of cortisol, thyroxine (T/sub 4/) and triiodothyronine (T/sub 3/) in pigs under two temperature and feeding regimes. Twenty-eight barrows were randomly assigned to one of the following: 1) ad-libitum fed at 5/sup 0/C(AL-5); 2) ad-libitum fed at 20/sup 0/C(AL-20); 3) meal fed at 5/sup 0/C(M-5); and 4) meal fed at 20/sup 0/C(M-20). M-5 and M-20 animals were fed at 0730 and 1400 hrs. Lights were on from 0600 to 2000 hrs. After 5 wks, blood samples were collected for 27 hrs. Serum cortisol, T/sub 4/ and T/sub 3/ concentrations were determined by RIA. No significant differences were found in the mesors, amplitudes or acrophases for cortisol. The mesors for T/sub 4/ (p<.01) were 60.6 +/- 5.6, 40.2 +/- 5.6, 61.2 +/- 5.6 and 49.1 +/- 5.0 ng/ml for AL-5, AL-20, M-5, and M-20, respectively. The mesors for T/sub 3/ (p<.01) were .85 +/- .06, .69 +/- .06, .92 +/- .06 and .66 +/- .05 ng/ml for AL-5, AL-20, M-5, ...

1986-03-05

Antihuman growth hormone (GH) antibodies cross-react with the GH-like factor from plerocercoids of the tapeworm Spirometra mansonoides.

Objective: To investigate the relationship between endothelial vasoactive factors and development of systemic sclerosis (SSc). Methods: Blood circulating endothelial cell count (CEC, with density gradient precipitation method); plasma endothelin (ET), thromboxane B2 (TXB2 ), 6-keto-prostaglandin F1? concentrations (all above three with RIA); nitric oxide (NO) contents (with Griss method) and plasma intercellular adhesion molecule-1 (ICAM-1), p-selectin (P-S) contents (with ELISA) were measured in 52 patients with systemic sclerosis (SSc) both before and after treatment with prostaglandin E1 and 30 controls. Results: Plasma ET, TXB2, ICAM-1, P-S contents and CEC were significantly higher and plasma NO, 6-K-PGF1? contents were significantly lower in all the patients with SSc before treatment than those in the controls, After treatment, the patients with satisfactory responses (n=33) had the abnormal plasma values of these parameters greatly corrected (vs before ...

2005-02-01