t-cell intrinsic expression: Topics by WorldWideScience.org

Reduced expression of a human V beta 6.1 T-cell receptor allele.

Although human T-cell lymphotropic virus type I (HTLV-I) is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), the role of viral gene expression in the progression to and maintenance of the...Full Text Available

1992-04-01

2

Early inflammatory markers in elicitation of allergic contact dermatitis

We have previously described an allelic polymorphism in the V beta 6.1 T-cell receptor gene. The V beta 6.1B allele is associated with disease in a subgroup of patients with juvenile rheumatoid arthritis....Full Text Available

1993-05-15

3

Identification of a functional nuclear export signal in the green fluorescent protein asFP499

BackgroundAllergic Contact Dermatitis (ACD) is regarded as a T-cell-mediated delayed-type hypersensitivity reaction. We studied the kinetics of the expression of CS-1 fibronectin,...Full Text Available

4

Intrinsic expression of host genes and intronic miRNAs in prostate carcinoma cells

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

5

Differentiation associated regulation of microRNA expression in vivo in human CD8⁺T cell subsets

BackgroundRecent data show aberrant and altered expression of regulatory noncoding micro (mi) RNAs in prostate cancer (PCa). A large number of miRNAs are encoded in organized intronic...Full Text Available

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Expression of bacterial luciferase in eukaryotic cells

BackgroundThe differentiation of CD8⁺T lymphocytes following priming of naïve cells is central in the establishment of the adaptive immune response. Yet, the...Full Text Available

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Melanopsin-expressing retinal ganglion-cell photoreceptors: cellular diversity and role in pattern vision

Expression of Bacterial luciferase enzyme (lux) in mammalian cells would be a powerful bioreporter protein system for in vivo imaging because eukaryotic luciferases need expensive substrates. However, only a few efforts have been made to express bacterial luciferase enzyme in mammalian cells. As the result of this, we attempted to construct bicistronic vector including two bacterial luciferase genes (LuxA and LuxB) for assessing the potential to be visualized in vitro or in vivo by optical imaging system after transfection to mammalian cells. We designed and synthesized luxA and luxB genes from Photorhabdus Luminescens. To co-express both luxA and luxB genes from a single promoter, we cloned as a bicistronic transcript fused with an internal ribosomal entry site (IRES). This bicistronic transcript was transfected by Superfect to HEK 293T cell line. We also transfected lux A and lux ...

2005-11-18

8

Exercise Decreases Myelin-Associated Glycoprotein Expression in the Spinal Cord and Positively Modulates Neuronal Growth

Using the photopigment melanopsin, intrinsically photosensitive retinal ganglion cells (ipRGCs) respond directly to light to drive circadian clock resetting and pupillary constriction. We now...Full Text Available

2010-07-15

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Development of melanopsin-based irradiance detecting circuitry

To successfully grow, neurons need to overcome the effects of hostile environments, such as the inhibitory action of myelin. We have evaluated the potential of exercise to overcome the intrinsic...Full Text Available

2007-07-01

10

Aminoglycoside Efflux in Pseudomonas aeruginosa: Involvement of Novel Outer Membrane Proteins

BackgroundMost retinal ganglion cells (RGCs) convey contrast and motion information to visual brain centers. Approximately 2% of RGCs are intrinsically photosensitive (ipRGCs), express...Full Text Available

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Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells

The expression of tripartite multidrug efflux pumps such as MexA-MexB-OprM in Pseudomonas aeruginosa contributes to intrinsic resistance to a wide variety of antimicrobials, including...Full Text Available

2003-03-01

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Ophthalmic abnormalities in patients with cutaneous T-cell lymphoma.

Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene. The plasmid pEGFP-optS, which contains the codon-optimized SARS-CoV S gene and expresses spike-EGFP fusion protein (S-EGFP) as silencing target and expressing reporter, was transfected with siRNAs into HEK 293T cells. At various time points of posttransfection, the levels of S-EGFP expression and amounts of spike mRNA transcript were detected by fluorescence microscopy, flow cytometry, Western blot, and real-time quantitative PCR, respectively. The results showed that the cells transfected with pEGFP-optS expressed S-EGFP fusion protein at a higher level compared with those transfected with pEGFP-S, which contains wildtype ...

2004-11-26

13

Interaction of an immunodominant epitope with Ia molecules in T-cell activation

PURPOSE: To determine the frequency of ophthalmic abnormalities in patients with cutaneous T-cell lymphoma (mycosis fungoides and Sézary syndrome) and T-cell lymphoma involving the skin and...Full Text Available

1998-01-01

14

Zebrafish embryo extracts promote sphere-forming abilities of human melanoma cell line

Udgivelsesdato: 1988-Jul

1988-01-01

15

British Library Electronic Table of Contents (United Kingdom)

Sphere-forming abilities in culture condition are considered a hallmark of cancer stem-like cells, which represents tumor cell invasiveness and stem-like characteristics. We aimed to show that the sphere-forming subpopulation of human malignant melanoma cell line WM-266-4 acts differently to zebrafish embryo extracts compared with their bulk counterpart. Spheres were maintained in neural stem cell culture conditions. The embryos of zebrafish at specific developmental stages were collected and the extracts were purified under 100 kDa. Spheres were treated with embyo extracts and proliferation assay and immunocytochemistry were conducted. Spheroid cells expressed nestin and epidermal growth factor receptor (EGFR) but not melanoma antigen recognized by T-cells (MART)1, indicating their stem-l...

2009-01-01

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Regulatory role of neuron-restrictive silencing factor in expression of TRPC1

Comparison of intrinsic and extrinsic tracer methods for estimating calcium bioavailability to rats from dairy foods

Neuron-restrictive silencer factor (NRSF) binds its consensus element to repress the transcription of various genes. The dominant-negative form (dnNRSF) has a hypertrophic effect on cardiogenesis through an unidentified mechanism. We examined the involvement of transient receptor potential (TRP) channel proteins, using transgenic mice overexpressing dnNRSF (dnNRSF mice). Electrophoretic mobility-shift assays revealed an interaction between NRSF and a neuron-restrictive silencer element-like sequence in intron 4 of TRPC1 genomic DNA. According to RT-PCR and Western analyses, TRPC1 was up-regulated in dnNRSF mouse heart. Transient overexpression of TRPC1 in HEK 293T cells increased the activity of the nuclear factor in activated T cells (NFAT) promoter and stimulated store-operated Ca"2"+ channel (SOCC)-mediated Ca"2"+ entry. Transfection of TRPC1 into primary cardiomyocytes increased NFAT activity, indicating a major role for TRPC1 in NFAT ...

2006-12-22

17

Genetic heterogeneity in human T-cell leukemia/lymphoma virus type II.

Dairy products doubly labeled with 45Ca and 47Ca were used to evaluate an extrinsic labeling procedure for calcium bioavailability determination. Nonfat milk, yogurt, and fresh cheese curd were prepared from caprine milk that was intrinsically labeled with 45Ca. The products were then labeled extrinsically with 47Ca and administered to rats by gavage. The 47Ca to 45Ca ratio in bone and teeth averaged about 1.00 with either milk, yogurt, or CaCl2, but the ratio was about 1.04 when dosed with cheese curd. Ca absorption, determined by whole-body counting of 47Ca, was lower (P less than 0.05) in cheese curd (59%) than in either milk (69%), yogurt (72%), or CaCl2 (72%). Expressed as percent of dose, the absorption of 47Ca was highly correlated with bone 47Ca (r = 0.973) and with bone 45Ca (r = 0.946). Correlation between tibia 47Ca and tibia 45Ca was r = 0.923. For the dairy products tested, our results indicated that extrinsic 47Ca was absorbed ...

18

Epidermal keratinocytes do not activate peripheral T-cells: interleukin-10 as a possible regulator

DNA from the peripheral blood mononuclear cells of 17 different individuals infected with human T-cell lymphoma/leukemia virus type II (HTLV-II) was successfully amplified by the polymerase chain reaction...Full Text Available

1993-03-01

19

The mitogenic activity of human T-cell leukemia virus type I is T-cell associated and requires the CD2/LFA-3 activation pathway.

The immunogenicity of allogeneic cultured human epidermal keratinocytes (cHEKs) has been studied in several models with contradictory results. We studied human T-cell activation in an in vitro...Full Text Available

2008-11-01

20

Chimeric Matrix Proteins Encoded by Defective Proviruses with Large Internal Deletions in Human T-Cell Leukemia Virus Type 1-Infected Humans

The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia...Full Text Available

1993-06-01

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Detection of constitutive heterodimerization of the integrin Mac-1 subunits by fluorescence resonance energy transfer in living cells

Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia/lymphoma (ATLL), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), and other diseases....Full Text Available

2000-05-01

22

The p75"N"T"R tumor suppressor induces cell cycle arrest facilitating caspase mediated apoptosis in prostate tumor cells

Macrophage differentiation antigen associated with complement three receptor function (Mac-1) belongs to #beta#_2 subfamily of integrins that mediate important cell-cell and cell-extracellular matrix interactions. Biochemical studies have indicated that Mac-1 is a constitutive heterodimer in vitro. Here, we detected the heterodimerization of Mac-1 subunits in living cells by means of two fluorescence resonance energy transfer (FRET) techniques (fluorescence microscopy and fluorescence spectroscopy) and our results demonstrated that there is constitutive heterodimerization of the Mac-1 subunits and this constitutive heterodimerization of the Mac-1 subunits is cell-type independent. Through FRET imaging, we found that heterodimers of Mac-1 mainly localized in plasma membrane, perinuclear, and Golgi area in living cells. Furthermore, through analysis of the estimated physical distances between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) fused to Mac-1 subunits, we ...

2006-08-04

23

Imaging immune response in vivo

The p75 neurotrophin receptor (p75"N"T"R) is a death receptor which belongs to the tumor necrosis factor receptor super-family of membrane proteins. This study shows that p75"N"T"R retarded cell cycle progression by induced accumulation of cells in G0/G1 and a reduction in the S phase of the cell cycle. The rescue of tumor cells from cell cycle progression by a death domain deleted (#DELTA#DD) dominant-negative antagonist of p75"N"T"R showed that the death domain transduced anti-proliferative activity in a ligand-independent manner. Conversely, addition of NGF ligand rescued retardation of cell cycle progression with commensurate changes in components of the cyclin/cdk holoenzyme complex. In the absence of ligand, p75"N"T"R-dependent cell cycle arrest facilitated an increase in apoptotic nuclear fragmentation of the prostate cancer cells. Apoptosis of p75"N"T"R expressing cells occurred via the intrinsic mitochondrial pathway leading to a ...

2006-03-24

24

Hsp22 (HspB8/H11) knockdown induces sam68 expression and stimulates proliferation of glioblastoma cells

PurposeClinical trials have commenced to evaluate the feasibility of targeting malignant gliomas with genetically engineered cytolytic T-cells (CTLs) delivered directly...Full Text Available

2008-06-15

25

British Library Electronic Table of Contents (United Kingdom)

Abstract Sam68 (Src-associated protein in mitosis 68-kDa) is a multifunctional protein, known to govern cellular signal transduction, transcription, RNA metabolism, proliferation, apoptosis, and HIV-1 replication. Although intrinsic mechanisms that modulate Sam68 function are beginning to emerge, the regulatory events contributing to its expression remain elusive. We previously reported that heat shock protein-22 (Hsp22) antagonizes Sam68 function in rev-response element (RRE)-mediated gene expression. We now demonstrate that Sam68 levels correlate inversely with Hsp22 in a variety of cells, including U87, Jurkat, 293T, and U-937. In U87 glioblastoma cells, which contained high levels of Hsp22 than other cell lines tested, Hsp22 knockdown dramatically increased both Sam68 mRNA and protein,...

2011-01-01

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Ontology and law in the early Poulantzas

British Library Electronic Table of Contents (United Kingdom)

This article reviews the little examined early work of the Greek Marxist and state theorist, Nicos Poulantzas (1936-1979). In his first book, Nature du choses et droit of 1965, the young scholar developed a sociology of law culled from the insights of philosophical ontology. The article sets out the central claims of that book and reflects on its place in Poulantzass intellectual development. Drawing on Heidegger, Sartre and Marx, Poulantzas proposed a species of Natural Law theory that unified `facts' and `values' by grounding legal concepts in a theory of social praxis centred on material labour. Legal categories were thus irreducible to ahistorical essences but were, rather, expressions of mankinds struggle to realize its intrinsic freedom. As we shall see, although flawed and in key re...

2009-01-01

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Interplay of the chirps and chirped pulse compression in a high-gain seeded free-electron laser

Special regulatory T-cell review: A rose by any other name: from suppressor T cells to Tregs, approbation to unbridled enthusiasm

In a seeded high-gain free-electron laser (FEL), where a coherent laser pulse interacts with an ultrarelativistic electron beam, the seed laser pulse can be frequency chirped, and the electron beam can be energy chirped. Besides these two chirps, the FEL interaction introduces an intrinsic frequency chirp in the FEL even if the above-mentioned two chirps are absent. We examine the interplay of these three chirps. The problem is formulated as an initial value problem and solved via a Green function approach. Besides the chirp evolution, we also give analytical expressions for the pulse duration and bandwidth of the FEL, which remains fully longitudinally coherent in the high-gain exponential growth regime. Because the chirps are normally introduced for a final compression of the FEL pulse, some conceptual issues are discussed. We show that to get a short pulse duration, an energy chirp in the electron beam is important.

2007-03-01

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Evaluation of T-cell receptor gene rearrangements in patients with recurrent patch/plaque (T2) CTCL (mycosis fungoides).

In the early 1970s a spate of papers by research groups around the world provided evidence for a negative regulatory role of thymus-derived lymphocytes (T cells). In 1971, Gershon and Kondo published...Full Text Available

2008-01-01

29

Clinical and laboratory experience of vorinostat (suberoylanilide hydroxamic acid) in the treatment of cutaneous T-cell lymphoma

Cutaneous T-cell lymphoma is typically a clonal neoplasm of epidermotropic CD4+ T-lymphocytes that includes the entity mycosis fungoides (MF). After identification of patients with recurrent MF treated...Full Text Available

1999-11-01

30

Autocrine regulation of T-cell activation by ATP release and P2X₇ receptors

The most common cutaneous T-cell lymphomas (CTCLs) – mycosis fungoides (MF) and Sézary Syndrome – are characterised by the presence of clonally expanded, skin-homing helper-memory...Full Text Available

2006-12-01

31

Overexpression of a maize dehydrin gene, ZmDHN2b, in tobacco enhances tolerance to low temperature

T-cell activation requires the influx of extracellular calcium, although mechanistic details regarding such activation are not fully defined. Here, we show that P2X₇ receptors play a key...Full Text Available

2009-06-01

32

British Library Electronic Table of Contents (United Kingdom)

Dehydrins, a subfamily of group 2 LEA proteins, are intrinsically unstructured plant proteins that accumulate in the late stages of seed development and in vegetative tissues subjected to water deficit, salinity, low temperature, or abscisic acid treatment. In this study, we isolated and characterized ZmDHN2b, a maize dehydrin gene. The genomic organization of the ZmDHN2b gene and its expression in maize seedlings were analyzed. To investigate the function of ZmDHN2b, we generated transgenic tobacco plants constitutively overexpressing ZmDHN2b. Ectopic expression of ZmDHN2b in tobacco accelerated seed germination and seedling growth at 15?C. Furthermore, ZmDHN2b-overexpressing lines had lower levels of cold-induced malondialdehyde and less electrolyte leakage than wild-type tobacco at 4?C....

2011-01-01

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An Intrinsic Baldwin Effect in the H Beta Broad Emission Line in the Spectrum of NGC 5548

Setting the standard in T-cell-depleted haploidentical transplantation and beyond

We investigate the possibility of an intrinsic Baldwin effect (i.e., nonlinear emission-line

2003-01-01

34

British Library Electronic Table of Contents (United Kingdom)

Much progress has been made in the clinical, biological and technical aspects of the T-cell-depleted haploidentical hematopoietic stem cell transplantation (HSCT). Our experience demonstrates that infusing a megadose of extensively T-cell-depleted hematopoietic peripheral blood stem cells after an immuno-myeloablative conditioning regimen in acute leukemia patients ensures sustained engraftment with minimal GvHD without the need of any post-transplant immunosuppressive treatment. Since our first successful pilot study, our efforts have concentrated on developing new conditioning regimens, optimizing the graft processing and improving the post-transplant immunological recovery. The results we have so far achieved in high risk acute leukemia patients show that haploidentical transplantation ...

2011-01-01

35

Hepatosplenic T-cell lymphoma is a distinct rare entity: Diagnosis by fine-needle aspiration cytology

British Library Electronic Table of Contents (United Kingdom)

Abstract In this brief report, we have described the fine-needle aspiration cytology (FNAC) of a hepatosplenic T-cell lymphoma (HSTCL) in a 60-year-old female patient. The patient complained of fever and heaviness of left upper abdomen. Ultrasound-guided FNAC of the spleen showed immature lymphoid cells, and a diagnosis of primary non-Hodgkin lymphoma of spleen was offered. Subsequently, the patient underwent splenectomy and wedge biopsy of liver that confirmed the cytological diagnosis. The lymphoma cells were positive for CD3 immunostain. Primary hepatosplenic T-cell lymphoma is a rare entity and may often pose diagnostic problems on FNAC. Diagn. Cytopathol. 2010. 2010 Wiley-Liss, Inc.

2011-01-01

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The intrinsic nature of the Baldwin effect

On the Asymmetrical Role of Intrinsic Defects in the Complementary/Opposing Oxide Formation/Dissolution Reactions.

The relationship between the fluxes of the two strongest UV emission lines and flux in the continuum

1992-01-01

37

Czech Academy of Sciences Repository

Full Text Available

1994-01-01

38

INTERRUPTED-GROWTH STUDIES OF THE SELF-ASSEMBLY OF INTRINSICALLY ACENTRIC SILOXANE-DERIVED MONOLAYERS

Beyond money : intrinsic work motivation in profit and nonprofit organizations

No abstract prepared.

2003-01-01

39

Norwegian Open Research Archives (NORA)

Full Text Available

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Transcriptomic analysis supports similar functional roles for the two thymuses of the tammar wallaby

The influence of MHC polymorphism on the selection of T-cell determinants of FMDV in cattle.

BackgroundThe thymus plays a critical role in the development and maturation of T-cells. Humans have a single thoracic thymus and presence of a second thymus is considered an anomaly....Full Text Available

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The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins

There is a quest for the development of a new generation of vaccines consisting of well-defined subunit antigens. For a number of practical reasons it is attractive to develop vaccines on the basis...Full Text Available

1995-01-01

42

Ia-antigen-T-cell interactions for a thymus-independent antigen composed of D amino acids.

BackgroundSensitization to house dust mite allergens is strongly correlated with asthma. Der p 7 elicits strong IgE antibody and T-cell responses in mite allergic...Full Text Available

2010-04-01

43

Evaluation of Two Homologous Proline-Rich Proteins of Coccidioides posadasii as Candidate Vaccines against Coccidioidomycosis?

Synthetic polypeptide antigens of L amino acids, although bearing repeating sequences, are thymus-dependent (L-TD), whereas the same polymers composed of D amino acids are thymus-independent (D-TI),...Full Text Available

1993-02-01

44

Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8⁺ T-Cells

Evaluation of the protective efficacy of recombinant T-cell-reactive proteins of Coccidioides posadasii in a murine model of coccidioidomycosis has led to the discovery of potential...Full Text Available

2007-12-01

45

Generation of human cortical neurons from a new immortal fetal neural stem cell line

Tick-borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. The disease is characterized by fever and debilitating encephalitis that can progress to chronic illness...Full Text Available

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Discovery of low-affinity preproinsulin epitopes and detection of autoreactive CD8 T-cells using combinatorial MHC multimers.

Isolation and expansion of neural stem cells (NSCs) of human origin are crucial for successful development of cell therapy approaches in neurodegenerative diseases. Different epigenetic and genetic immortalization strategies have been established for long-term maintenance and expansion of these cells in vitro. Here we report the generation of a new, clonal NSC (hc-NSC) line, derived from human fetal cortical tissue, based on v-myc immortalization. Using immunocytochemistry, we show that these cells retain the characteristics of NSCs after more than 50 passages. Under proliferation conditions, when supplemented with epidermal and basic fibroblast growth factors, the hc-NSCs expressed neural stem/progenitor cell markers like nestin, vimentin and Sox2. When growth factors were withdrawn, proliferation and expression of v-myc and telomerase were dramatically reduced, and the hc-NSCs differentiated into glia and neurons (mostly glutamatergic and ...

2007-02-01

47

Large-scale Analysis of Thermo-stable, Mammalian Proteins Provides Insights into the Intrinsically Disordered Proteome

Autoreactive cytotoxic CD8 T-cells (CTLs) play a key pathogenic role in the destruction of insulin-producing beta-cells resulting in type 1 diabetes. However, knowledge regarding their targets is limited, restricting the ability to monitor the course of the disease and immune interventions. In a multi-step discovery process to identify novel CTL epitopes in human preproinsulin (PPI), PPI was digested with purified human proteasomes, and resulting COOH-fragments aligned with algorithm-predicted HLA-binding peptides to yield nine potential HLA-A1, -A2, -A3 or -B7-restricted candidates. An UV-exchange method allowed the generation of a repertoire of multimers including low-affinity HLA-binding peptides. These were labeled with quantum dot-fluorochromes and encoded in a combinatorial fashion, allowing parallel and sensitive detection of specific, low-avidity T-cells. Significantly increased frequencies of T-cells against four ...

2011-05-31

48

Intrinsic Factor-mediated Absorption of Cobalamin by Guinea Pig Ileal Cells

Intrinsically disordered proteins are predicted to be highly abundant and play broad biological roles in eukaryotic cells. In particular, by virtue of their structural malleability and propensity...Full Text Available

2009-01-01

49

Research in Reliability, Availability and Maintainability for ...

To investigate the fate of intrinsic factor and cobalamin during cobalamin absorption, we incubated enterocytes isolated from guinea pig ileum for periods of up to 30 min with ⁵⁷Co-labeled...Full Text Available

1983-03-01

50

Joint Sensor: Security Test and Evaluation Embedded in a ...

... Effect of checkpointing and queueing on program performance ... and a large class of stochastic linear ... problem is intrinsically related to K-terminal ...

1990-01-01

51

Strategies to optimize the outcome of children given T-cell depleted HLA-haploidentical hematopoietic stem cell transplantation

... from theory to practice will be significantly reduced while intrinsically revolutionizing the approach to engineering network security architectures. ...

2010-12-01

52

British Library Electronic Table of Contents (United Kingdom)

The most advanced frontier of allogeneic hematopoietic stem cell transplantation (allo-HSCT) is represented by the use of an HLA-partially matched relative as donor. In this type of transplantation, donor-derived natural killer (NK) cells, which are alloreactive towardtoward recipient cells, significantly contribute to the eradication of leukemia blasts. Alloreactive NK cells may also kill host dendritic cells and T lymphocytes, thus preventing graft-versus-host disease and graft rejection, respectively. Sophisticated strategies of adoptive infusion of T-cell lines/clones specific for the most life-threatening pathogens (namely cytomegalovirus, Epstein-Barr virus, Aspergillus and Adenovirus) have been envisaged, and successfully tested in a few pilot trials, to protect the recipient in the...

2011-01-01

53

Herpesvirus saimiri infection of rhesus macaques: A model for acute rhadinovirus-induced t-cell transformation and oncogenesis

British Library Electronic Table of Contents (United Kingdom)

Abstract Herpesvirus saimiri (HVS) causes acute lymphoma and leukemia upon experimental infection of various monkey species. HVS strain C488 is also capable of transforming human T-lymphocytes to stable growth in culture. The most susceptible species for oncogenesis are New World primates, in particular the cottontop tamarin (Saguinus oedipus). However, Old World monkeys such as macaques are the most used animal model for the close-to-human situation. The limited data on HVS infection in Old World monkeys prompted us to investigate susceptibility to infection and disease induction by HVS in macaques. After having established that rhesus macaques can be infected productively, and that rhesus T-cells can be transformed in vivo by HVS, we observed induction of lymphoma in all inoculated anima...

2011-01-01

54

Viral suppression of multiple escape mutants by de novo CD8⁺T cell responses in a human immunodeficiency virus-1 Infected elite suppressor

Vaccination response to tetanus toxoid and 23-valent pneumococcal vaccines following administration of a single dose of abatacept: a randomized, open-label, parallel group study in healthy subjects

Elite suppressors or controllers (ES) are HIV-1 infected patients who maintain undetectable viral loads without treatment. While HLA-B*57-positive ES are usually infected with virus that is unmutated...Full Text Available

55

SIV antigen immunization induces transient antigen-specific T cell responses and selectively activates viral replication in draining lymph nodes in retroviral suppressed rhesus macaques

The effect of abatacept, a selective T-cell co-stimulation modulator, on vaccination has not been previously investigated. In this open-label, single-dose, randomized, parallel-group, controlled study,...Full Text Available

2007-01-01

56

Mapping of TH1 helper T-cell epitopes on major secreted mycobacterial antigen 85A in mice infected with live Mycobacterium bovis BCG.

BackgroundHIV infection causes a qualitative and quantitative loss of CD4⁺T cell immunity. The institution of anti-retroviral therapy (ART) restores CD4⁺T...Full Text Available

57

Long-Term Specific Immune Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine: Characterization of CD8⁺-T-Cell Epitopes Recognized

TH1 cytokine secretion was examined in response to synthetic peptides of the 85A component of the major secreted, fibronectin-binding antigen 85 complex from Mycobacterium tuberculosis in seven different...Full Text Available

1994-02-01

58

Helper T-Cell Epitopes Encoded by the Babesia bigemina rap-1 Gene Family in the Constant and Variant Domains Are Conserved among Parasite Strains

We studied the effect of booster injections and the long-term immune response after injections of an anti-human immunodeficiency virus type 1 (HIV-1) lipopeptide vaccine. This vaccine was injected alone...Full Text Available

2003-10-01

59

Extracorporeal Photochemotherapy as a Challenging Treatment for Cutaneous T-Cell Lymphoma, Acute and Chronic Graft-versus-Host Disease, Organ Rejection and T-Lymphocyte-Mediated Autoimmune Diseases

Among important candidates for babesial vaccines are apical complex proteins, including rhoptry-associated protein 1 (RAP-1) from Babesia bovis and B. bigemina, which...Full Text Available

1998-04-01

60

Differential Specificity and Immunogenicity of Adenovirus Type 5 Neutralizing Antibodies Elicited by Natural Infection or Immunization?

Summary20 years ago, in 1987, Edelson and co-workers published their first report on the effectiveness of a new procedure, called extracorporeal photochemotherapy (ECP), in patients...Full Text Available

2008-01-01

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CD5 Is Dissociated from the B-Cell Receptor in B Cells from Bovine Leukemia Virus-Infected, Persistently Lymphocytotic Cattle: Consequences to B-Cell Receptor-Mediated Apoptosis

A recent clinical trial of a T-cell-based AIDS vaccine delivered with recombinant adenovirus type 5 (rAd5) vectors showed no efficacy in lowering viral load and was associated with increased risk of...Full Text Available

2010-01-01

62

Recent Therapy for Reversible Airway Disease

Bovine leukemia virus (BLV), a retrovirus related to human T-cell leukemia virus types 1 and 2, can induce persistent nonneoplastic expansion of the CD5⁺ B-cell population, termed...Full Text Available

2001-02-01

63

Intrinsic Motivation and Learning in a Schizophrenia Spectrum Sample

The current therapy for extrinsic and intrinsic asthma, together with that of ‘wheezing bronchitis’, is presented, with special attention to bronchodilators and adrenocorticosteroids....Full Text Available

1976-03-01

64

Intrinsic Acidity of Aluminum, Chromium (III), and Iron (III) mu(3)-Hydroxo Functional Groups from ab Initio Electronic Structure Calculations.

Amotivation is a telling hallmark of negative symptomatology in schizophrenia, and it impacts nearly every facet of behavior, including inclination to attempt the difficult cognitive tasks involved...Full Text Available

2010-05-01

65

Intrinsic and extrinsic crossluminescence in ionic crystals

There is no abstract currently available for this item

2000-05-15

66

Intrinsic and extrinsic crossluminescence in ionic crystals

Intrinsic crossluminescence (CRL) of CsBr, CsCl, and of BaF{sub 2} was investigated with electron-beam and synchrotron radiation excitation. From the CRL spectra, the excitation spectra and the reflectivity, energy level schemes were deduced. Extrinsic CRL was observed changing either the initial (CsCl:Br{sup -}) or the final (RbCl:Cs{sup +}; KCl:Cs{sup +}) state of CRL by doping. (author).

1991-01-01

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Total skin electron beam and total nodal irradiation for treatment of patients with cutaneous T-cell lymphoma

Intrinsic crossluminescence (CRL) of CsBr, CsCl, and of BaF_2 was investigated with electron-beam and synchrotron radiation excitation. From the CRL spectra, the excitation spectra and the reflectivity, energy level schemes were deduced. Extrinsic CRL was observed changing either the initial (CsCl:Br"-) or the final (RbCl:Cs"+; KCl:Cs"+) state of CRL by doping. (author).

1990-09-03

68

Human T-cell lymphotropic virus type III infection of the central nervous system: a preliminary in situ analysis

Sixteen patients with advanced cutaneous T-cell lymphoma (CTCL) with or without lymph node involvement, but without evidence of extranodal manifestations, were treated with a combination of total skin electron beam therapy (TSEB) and total nodal irradiation (TNI). Fourteen (87%) patients achieved a complete response (CR) lasting from 1 to 84+ months (median, 8+ months) from the completion of treatment. The best results occurred in 6 patients with pretumorous intracutaneous CTCL (Stages IB and IIA) where the CR has lasted in all patients from 8 to 84+ months (median about 27+ months). Radiotherapy was well tolerated with the major toxicity being bone marrow suppression. The authors conclude that combined TSEB and TNI is a relatively safe and effective treatment for patients with CTCL prior to the development of lymph node involvement. Long-term follow-up is needed to assess the curative potential of this treatment.

1985-06-01

69

Copper-65-absorption by men fed intrinsically and extrinsically labeled whole wheat bread

Patients with acquired immunodeficiency syndrome (AIDS) are subject to a spectrum of central nervous system (CNS) disorders. Recent evidence implicates the human T-cell lymphotropic virus type III (HTLV-III) in the pathogenesis of some of these illnesses, although the cells infected by the virus have yet to be identified. Using in situ hybridization, the authors examined brain tissue from two patients with AIDS encephalopathy for the presence of HTLV-III RNA. In both cases, viral RNA was detected and concentrated in, though not limited to, the white matter. The CNS cells most frequently infected included macrophages, pleomorphic microglia, and multinucleated giant cells. Less frequently, cells morphologically consistent with astrocytes, oligodendroglia, and rarely neurons were also infected. The findings strengthen the association of HTLV-III with the pathogenesis of AIDS encephalopathy. In situ hybridization can be applied to routinely prepared biopsy tissue in ...

1986-11-07

70

Dual-field rotational (DFR) technique for total-skin electron-beam therapy (TSEBT)

Six men were fed a diet composed of conventional foods with all bread as whole wheat bread. Intrinsically labeled "6"5Cu bread (containing 6.5 ppm Cu and 48 atom % "6"5Cu) was substituted for unlabeled bread for 3 days, and stools were collected for 24 days. Extrinsically labeled bread was then substituted for 3 days and another 24-day stool collection made. "6"5Cu excretion was measured by mass spectrometry. Mean Cu intake was 1.10 mg of Cu/day. Average Cu balance was /minus/0.06 /+-/ 0.08 mg/day. Average absorption of the intrinsic copper was 72.2 /+-/ 9.3% and of extrinsic Cu 64.2 /+-/ 5.8%. The ratio of extrinsic to intrinsic absorption was 0.906 /+-/ 0.164. Absorption of intrinsic and extrinsic tracers did not differ significantly (p > 0.05) by a paired t-test, and the ratio (E/I) was not significantly different from 1. Use of extrinsic Cu tracers to assess Cu absorption is supported by these ...

71

Expression of retinoic acid receptor beta in dermatofibrosarcoma protuberans.

Comparison of dosimetry measured in a noncylindrical Rando-Alderson phantom by two-field, four-field, and six-field total-skin electron-beam therapy (TSEBT) techniques with our dual-field rotational (DFR) technique reveals a superior dosimetry for the latter. Our technique of dual-field rotational DFR-TSEBT is described in detail, and its advantages and indications for the primary management of cutaneous T-cell lymphoma (CTCL) and Kaposi's sarcoma (KS) are discussed.

1987-08-01

72

Expression of p63 in reactive hyperplasias and malignant lymphomas.

PMID:19469866

2009-03-16

73

Endodermal sinus tumor: immunophenotypic expression of a carcinoma.

Full Text Available

2005-10-01

74

The future of dentistry: new challenges, new directions.

Full Text Available

1994-04-01

75

Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy

The challenge to our profession today is to improve the quality of oral health while overcoming both extrinsic and intrinsic factors which may adversely affect our progress toward this goal. The combined...Full Text Available

1990-05-01

76

Protracted withdrawal from alcohol and drugs of abuse impairs long-term potentiation of intrinsic excitability in the juxtacapsular bed nucleus of the stria terminalis

BackgroundThe circadian rhythm in mammals is orchestrated by a central pacemaker in the brain, but most peripheral tissues contain their own intrinsic circadian oscillators. The...Full Text Available

77

Lnk constrains myeloproliferative diseases in mice

The juxtacapsular BNST (jcBNST) is activated in response to basolateral amygdala (BLA) inputs through the stria terminalis and projects back to the anterior BLA and to the central nucleus of...Full Text Available

2009-04-29

78

Involuntary Mental Time Travel and Its Effect on Prospective Teachers' Situational Intrinsic Motivations

Hematopoietic stem and progenitor cell (HSPC) expansion is regulated by intrinsic signaling pathways activated by cytokines. The intracellular kinase JAK2 plays an essential role in cytokine signaling,...Full Text Available

2010-06-01

79

Intrinsic plasticity complements LTP in parallel fiber input gain control in cerebellar Purkinje cells

Recent cognitive psychological research has argued that involuntary mental time travel is an important individual difference variable that has the potential to affect an individual's motivation. However, this issue has not been empirically investigated in educational settings such as teacher education. Therefore, this study aimed to explore the possible effect of involuntary mental time travel on prospective teachers' situational intrinsic motivations in the classrooms during the period of a class hour. A total of 274 prospective teachers voluntarily participated in the study. Results showed that involuntary mental time travel into the past and into the future occurred in the classrooms even during the period of a class hour. The characteristics of the prospective teachers' involuntary memories/future images were evident. Most importantly, results revealed that the teaching-related positive future images significantly and positively affected the prospective ...

2010-12-01

80

Intrinsic near-24-h pacemaker period determines limits of circadian entrainment to a weak synchronizer in humans

Synaptic gain control and information storage in neural networks are mediated by alterations in synaptic transmission, such as in long-term potentiation (LTP). Here, we show using both in...Full Text Available

2010-10-13

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

81

INTRINSIC DOSIMETRY: A POTENTIAL NEW TOOL FOR NUCLEAR FORENSICS INVESTIGATIONS

Endogenous circadian clocks are robust regulators of physiology and behavior. Synchronization or entrainment of biological clocks to environmental time is adaptive and important for physiological homeostasis...Full Text Available

2001-11-20

82

Homeostasis of intrinsic excitability in hippocampal neurones: dynamics and mechanism of the response to chronic depolarization

Thermoluminescence (TL) dosimetry was used to measure dose effects on the raw stock material of borosilicate container glass from different geographical locations. Effects were studied at times up to 60 days post-irradiation at doses from 0.15 to 20 Gy. The minimum detectable dose using this technique was estimated to be 0.15 Gy which is roughly equivalent to a 24 hr irradiation 1 cm from a 50 ng source of 60Co. Two peaks were identified in the TL glow curve, a relatively unstable peak around 125 C and a more stable peak around 225 C. Differences in TL glow curve shape and intensity were also observed for the glasses from different geographical origins. We investigate radiation induced defects in glass to further develop the technique of intrinsic dosimetry - the measurement of the total absorbed dose received by the walls of a container holding radioactive material. Intrinsic dosimetry is intended to be used as an interrogation tool to provide ...

2010-07-11

83

Comparison of Detector Intrinsic Spatial Resolution Characteristics for Sensor on the Entrance Surface and Conventional Readout Designs

In order to maintain stable functionality in the face of continually changing input, neurones in the CNS must dynamically modulate their electrical characteristics. It has been hypothesized that in...Full Text Available

2010-01-01

84

Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility

We report on a high resolution, monolithic crystal PET detector design concept that provides depth of interaction (DOI) positioning within the crystal. Our design utilizes a novel sensor on...Full Text Available

2010-01-01

85

An Intrinsic Neuronal Oscillator Underlies Dopaminergic Neuron Bursting

Integumentary wounds in mammalian fetuses heal without scar; this scarless wound healing is intrinsic to fetal tissues and is notable for absence of the contraction seen in postnatal (adult) wounds....Full Text Available

86

A generalized Fisher equation and its utility in chemical kinetics

Dopaminergic neurons of the ventral midbrain fire high frequency bursts when animals are presented with unexpected rewards, or stimuli that predict reward. To identify the afferents that can...Full Text Available

2009-12-16

87

Radius of gyration and intrinsic viscosity of polyelectrolyte solutions

A generalized Fisher equation (GFE) relates the time derivative of the average of the intrinsic rate of growth to its variance. The GFE is an exact mathematical result that has been widely used in population...Full Text Available

2010-07-20

88

Polarized proton acceleration at the Brookhaven AGS

Relatively low molecular weights polyelectrolytes (10{sup 4}-10{sup 6}) behave as worm-like chain when electrostatic repulsions are assumed to govern the excluded volume parameter. Under such conditions, predictions of chain expansion and effect of polyelectrolyte concentrations are made assuming that unperturbed dimensions could be obtained at infinite salt content. Experimental studies of an ionic polysaccharide, namely the Na-hyaluronate, were done and the values obtained for the radius of gyration as well as the intrinsic viscosity at different charge densities are in good agreement with the predictions.

1993-12-31

89

Treatment of hematological malignancies with nonmyeloablative, HLA-haploidentical bone marrow transplantation and high dose, post-transplantation cyclophosphamide

At the conclusion of polarized proton commissioning in February 1986, protons with an average polarization of 45%, momentum of 21.7 GeV/c, and intensity of 2 x 10/sup 10/ protons per pulse, were extracted to an external polarimeter at the Brookhaven AGS. In order to maintain this polarization, five intrinsic and nearly forty imperfection depolarizing resonances had to be corrected. An apparent interaction between imperfection and intrinsic resonances occurring at very nearly the same energy was observed and the correction of imperfection resonances using ''beat'' magnetic harmonics discovered in the previous AGS commissioning run was further confirmed.

1986-01-01

90

British Library Electronic Table of Contents (United Kingdom)

Hematopoietic stem cell transplantation provides the only potential curative option in many patients with hematological malignancies. Finding a suitably matched donor in a timely manner is often difficult. However, most patients have a partially HLA-mismatched (HLA-haploidentical) first-degree relative readily available. Historically, HLA-haploidentical bone marrow transplantation (BMT) has been considered extremely high risk due to high rates of life-threatening graft-versus-host disease (GVHD) and non-relapse mortality (NRM). Modifications of the stem cell graft, such as T-cell depletion, have resulted in poor rates of engraftment. We have recently completed a phase II clinical trial of nonmyeloablative HLA-haploidentical hematopoietic BMT followed by post-transplantation high-cyclophosp...

2011-01-01

91

Role of regulatory T cell populations in controlling graft vs host disease

British Library Electronic Table of Contents (United Kingdom)

Immune function is critical in health and disease. The control and regulation of immune reactions is an area of intense investigation that has important implications for allogeneic hematopoietic cell transplantation. Immune reactions are regulated in a number of important ways. Compartmentalization of immune responses and the production of both pro-inflammatory and anti-inflammatory cytokines play a major role. More recently several populations of T cells that regulate immune responses termed regulatory T cells have been identified. This manuscript will focus on CD4^+CD25^+FoxP3^+ natural regulatory T cells (T"r"e"g) and @a@bTCR^+CD4^+NK1.1^+ natural killer T (NK-T) cells which both suppress graft vs host disease but appear to function by distinct mechanisms.

2011-01-01

92

Nickel induced structural and functional alterations in mouse Leydig cells in vitro

British Library Electronic Table of Contents (United Kingdom)

The present study was aimed at investigating effects of nickel (NiCl2) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000mmol/L NiCl2 or without NiCl2 using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of >=250mmol/L NiCl2. A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000mmol/L NiCl2. After ...

2011-01-01

93

Estimates of quantitative genetic parameters of immunological traits in the chicken.

Decreased semen quality in a male infected with malaria.

Three in vivo assays were used to measure the immunocompetence of chickens in two generations of a selection experiment. The obtained data were used to estimate the variance components for sire and dam for antibody production to Pasteurella multocida and Mycoplasma gallisepticum vaccines, for T-cell-mediated immunity evaluated by a phytohemagglutinin wing web assay, and for clearance of foreign particles from the circulatory system. Heritabilities of and genetic correlations among these immunological traits were calculated from the sire variance components. Heritability estimates of the immunological traits based on the sire component of variance ranged from .06 to .53, and genetic correlations among immunological traits were generally negative. PMID:1956847

1991-10-01

94

A novel BMT technique for treatment of various currently intractable diseases

A 33-year-old male of proven fertility suffered six attacks of malaria while resident in an African country. For this he received anti-malarial drugs. Semen analysis performed after the fourth attack, and repeated during the following 2 years after his return to Israel, revealed severe oligozoospermia, necrozoospermia and occasionally even azoospermia. Immunological examination of the patient revealed an inverse ratio of T-cell subsets and mast cell degranulation in response to palludrin. Twenty-five months after the last attack of malaria a significant improvement was found in semen quality and there was an increase in the ratio of T-helper to T-suppressor cytotoxic cells. These observations indicate that although malaria and its treatment may affect spermatogenesis, recovery may be expected eventually. PMID:3500921

1987-10-01

95

British Library Electronic Table of Contents (United Kingdom)

A recently-developed BMT method combines a ''Perfusion Method'' (PM) for collecting bone marrow cells (BMCs) with the Intra-Bone Marrow (IBM) injection of BMCs (IBM-BMT). As distinct from the conventional aspiration method (AM), the PM allows rapid (within 1 h) collection of BMCs without T cell contamination (T cells < 10%). Therefore, no GvHD occurs. Moreover, the burden on donors, such as back pain, bleeding and infection, can be reduced. Full chimerism can be achieved even with only mild conditioning regimens if IBM-BMT is carried out, since IBM-BMT replaces not only the recipient's hemopoietic stem cells (HSCs) but also mesenchymal stem cells (MSCs) with donor-derived HSCs and MSCs. Using this method, we show that most currently intractable diseases are HSC or MSC disorders, and that t...

2011-01-01

96

Tuning and controlling gene expression noise in synthetic gene networks

Growth-Phase-Dependent Expression of Virulence Factors in an M1T1 Clinical Isolate of Streptococcus pyogenes

Synthetic gene networks can be used to control gene expression and cellular phenotypes in a variety of applications. In many instances, however, such networks can behave unreliably due to gene expression...Full Text Available

2010-05-01

97

Expression of p53 in leukoplakia and squamous cell carcinoma of the oral mucosa: correlation with expression of Ki67

The effect of growth phase on expression of virulence-associated factors was studied by Northern hybridization in an M1T1 clinical isolate of Streptococcus pyogenes. Expression of M...Full Text Available

1999-10-01

98

EDISA: extracting biclusters from multiple time-series of gene expression profiles

Aim—To study p53 expression in relation to proliferative status in normal and nondysplastic, dysplastic and malignant lesions of the oral mucosa.Method—The...Full Text Available

1996-06-01

99

Detecting microRNA activity from gene expression data

BackgroundCells dynamically adapt their gene expression patterns in response to various stimuli. This response is orchestrated into a number of gene expression modules consisting...Full Text Available

100

Antisense Expression of the CK2 ?-Subunit Gene in Arabidopsis. Effects on Light-Regulated Gene Expression and Plant Growth1

BackgroundMicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression by binding to the messenger RNA (mRNA) of protein coding genes. They control gene expression by either...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

101

A statistical framework for modeling gene expression using chromatin features and application to modENCODE datasets

The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because...Full Text Available

1999-03-01

102

A retinoid responsive cytokine gene, MK, is preferentially expressed in the proximal tubules of the kidney and human tumor cell lines.

We develop a statistical framework to study the relationship between chromatin features and gene expression. This can be used to predict gene expression of protein coding genes, as well as microRNAs....Full Text Available

2011-01-01

103

Development of radiological emergency preparedness technology

The aim of this study was to survey the expression of an embryonic cytokine gene, MK, in the normal organs and neoplastic tissues of adults. Northern analysis showed that MK mRNA was exclusively expressed...Full Text Available

1993-02-01

104

Stochastic gene expression and its consequences

Large-scale field tracer experiments have been conducted on Ulchin, Wolsung and Daeduk sites for the purpose of validating FADAS and of analyzing the environmental characteristics around the nuclear sites. The most influential factor in atmospheric dispersion is the meteorological condition. During the experiment, meteorological data were measured on the release point and the selected positions among sampling points. Once radioactive materials are released to the atmosphere, members of public may be exposed through the environmental media such as air, soil and foods. Therefore, to protect the public, adequate countermeasures should be taken at due time for those exposure pathways. both processes, of justification and optimization are applied to a countermeasure simultaneously for decision-making. The work scope of Biological research for the radiation protection had contained the search of biological microanalytic methods for assessing the health effect by {gamma}-radiation and toxic ...

2000-04-01

105

Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male ...

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

106

Localised breast cancers may have systemic influences on skin and hair

... 041889 Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male Reproducti...

107

Expression of cell proliferation and apoptosis biomarkers in pterygia and normal conjunctiva

HypothesisBiomarkers, commonly expressed in breast cancer cells, may be correlated with their expression in breast skin of the same subjects.MethodsThe...Full Text Available

2007-02-01

108

ERG Expression Levels in Prostate Tumors Reflect Functional ...

PurposeTo analyze the expression of apoptosis and cell proliferation molecules in pterygium tissues of Chinese patients.MethodsThirty-three pterygia...Full Text Available

109

Cis-regulatory mutations in human disease

... Title : ERG Expression Levels in Prostate Tumors Reflect Functional Status of the Androgen Receptor (AR) as a Consequence of Fusion of ERG ...

110

THE M-#sigma# AND M-L RELATIONS IN GALACTIC BULGES, AND DETERMINATIONS OF THEIR INTRINSIC SCATTER

Cis-acting regulatory sequences are required for the proper temporal and spatial control of gene expression. Variation in gene expression is highly heritable and a significant determinant...Full Text Available

2009-07-01

111

Comparison of two dose-area-product ionization chambers with different conductive surface coating for over-table and under-table tube configurations

We derive improved versions of the relations between supermassive black hole mass (M _B_H) and host-galaxy bulge velocity dispersion (#sigma#) and luminosity (L; the M-#sigma# and M-L relations), based on 49 M _B_H measurements and 19 upper limits. Particular attention is paid to recovery of the intrinsic scatter (#epsilon#_0) in both relations. We find log(M _B_H/M _s_u_n) = #alpha# + #beta#log(#sigma#/200 km s"-"1) with (#alpha#, #beta#, #epsilon#_0) = (8.12 #+-# 0.08, 4.24 #+-# 0.41, 0.44 #+-# 0.06) for all galaxies and (#alpha#, #beta#, #epsilon#_0) = (8.23 #+-# 0.08, 3.96 #+-# 0.42, 0.31 #+-# 0.06) for ellipticals. The results for ellipticals are consistent with previous studies, but the intrinsic scatter recovered for spirals is significantly larger. The scatter inferred reinforces the need for its consideration when calculating local black hole mass function based on the M-#sigma# relation, and further implies that there may be ...

2009-06-10

112

Segmented focal plane detector for light and heavy ions

A custom-built graphite-coated transmission ionization chamber is compared to the VacuDAP 2001 (VacuTec, Dresden, Germany), which has transparent conductive electrodes. A study was made of the dependence of response on x-ray tube potential for both types of chamber under identical conditions of exposure using over-table and under-table x-ray tubes. Since the calibration factor is the dose-area product of the radiation incident on the patient per chamber reading, it depends on the intrinsic response of the chamber as well as the effect of material in the beam between the x-ray tube and patient. Differences of about 20% were measured between the intrinsic and the over-table calibration factors and between the over-table and the under-table calibration factors for both chambers. The VacuDAP display is specifically calibrated for the over-table condition and would overstate the actual DAP in the under-table case. The intrinsic ...

2000-03-01

113

Intrinsic fluorescence of the amniotic fluid

A segmented focal plane detector for an Enge split-pole spectrograph has been developed for the study of breakup reactions at very low relative energies. It consists of a 61 cm long segmented position-sensitive parallel plate avalanche counter backed by a large Bragg curve detector. A segmented plastic scintillator is mounted behind the anode of the Bragg curve detector and is used for particle identification of low-ionizing particles. The dead space between the two sections of the focal plane detector is 2.5 mm. The intrinsic position resolution of the detector is 1 mm. The intrinsic energy resolution depends on the energy of the incident ion and can be as good as 0.55%. The nuclear charge and mass resolutions are 0.3 e and 0.3 u, respectively. (orig.).

1992-06-15

114

Energy levels in /sup 238/Np and /sup 240/,/sup 242/,/sup 244/Am based on residual interaction studies

The intrinsic fluorescence of clear amniotic fluid from third-trimester pregnancy is reported. The fluorescence intensity peaked at 405 nm when excited by ultraviolet light in the range of 310 to 360 nm. All of the 33 samples tested fluoresced, and their emission spectrum had the same shape, varying only in magnitude. Changing sample temperature (20 to 50 C) and sample pH (7.2 to 7.7) had little effect on the emission spectrum. The same fluorescence, with higher intensity, was observed when some newborn urine was tested. The fluorescence of amniotic fluid may provide a new tool to determine fetal age, fetal organ maturation, and fetal distress. (author).

1981-01-01

115

The intrinsic energy levels in the doubly odd isotopes of Np with mass number 238 and of Am with mass numbers 240, 242 and 244 are calculated using the zero-range neutron-proton residual interaction whose parameters are evaluated using the atomic masses and the ground state Gallagher-Moszkowski splitting energy in each case. A configuration dependence of the residual interaction is in evidence in several cases. The results are compared with the experimental data and the comparison is used to check on the two-particle configuration assignments to the intrinsic states in the respective spectra. Encouraged by the nature and the degree of the agreement of the theory with the available experimental data, predictions are made about the expected location of the as yet unobserved two-quasiparticle states in these nuclei.

1982-01-18

116

Do Spinors Frame-Drag?

Cosmic magnification: nulling the intrinsic clustering signal

We investigate the effect of the intrinsic spin of a fundamental spinor field on the surrounding spacetime geometry. We show that despite the lack of a rotating stress-energy source (and despite claims to the contrary) the intrinsic spin of a spin-half fermion gives rise to a frame-dragging effect analogous to that of orbital angular momentum, even in Einstein-Hilbert gravity where torsion is constrained to be zero. This resolves a paradox regarding the counter-force needed to restore Newton's third law in the well known spin-orbit interaction. In addition, the frame-dragging effect gives rise to a {\\it long-range} gravitationally mediated spin-spin dipole interaction coupling the {\\it internal} spins of two sources. We argue that despite the weakness of the interaction, the spin-spin interaction will dominate over the ordinary inverse square Newtonian interaction in any process of sufficiently high-energy for quantum field theoretical ...

2009-01-01

117

VEGF receptor mRNA expression by ACL fibroblasts is associated with functional healing of the ACL.

We investigate the extent to which the pure magnification effect of gravitational lensing can be extracted from galaxy clustering statistics, by a nulling method which aims to eliminate terms arising from the intrinsic clustering of galaxies. The aim is to leave statistics which are free from the uncertainties of galaxy bias. We find that nulling can be done effectively, leaving data which are relatively insensitive to uncertainties in galaxy bias and its evolution, leading to cosmological parameter estimation which is effectively unbiased. This advantage comes at the expense of increased statistical errors, which are in some cases large, but it offers a robust alternative analysis method to cosmic shear for cosmological imaging surveys designed for weak lensing studies, or to full modelling of the clustering signal including magnification effects.

2011-01-01

118

The extracellular calcium sensing receptor is expressed in mouse mesangial cells and modulates cell proliferation.

PMID:21331648

2011-02-19

119

Staphylococcus haemolyticus Lipase; High-Level Expression in Escherichia coli and Activation of Nonionic Detergent

Full Text Available

2005-10-01

120

Promoter Structure Which Affects on the Expression of Yeast MGMT Gene

Full Text Available

2000-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

121

Parallel expression of synaptophysin and evoked neurotransmitter release during development of cultured neurons

Full Text Available

1997-01-01

122

Morphine postconditioning attenuates icam-1 expression on endothelial cells.

Udgivelsesdato: 1991-null

1991-01-01

123

Immunohistochemical expression of p63 in human prenatal tooth primordia

Full Text Available

2011-02-01

124

GENE EXPRESSION VECTOR USING INSULATOR ORIGINATED FROM SEA URCHIN

development, histochemical, human, malformation, p63 gene, tooth

2005-01-01

125

J-STORE (Japan)

Full Text Available

2010-03-05

126

Expression of microtubule associated protein of low molecular weight (TAU) in mouse brain development

Expression and Characterization of a New Esterase Cloned Directly from Agrobacterium tumefaciens Genome

Published in summary form only.

127

Enhanced Expression of Chitinase during the Autolysis of Mushroom in Coprinellus congregatus

Full Text Available

2006-01-01

128

Construction of the Transgenic Drosophila melanogaster Expressing a Human Megsin Gene.

Full Text Available

2009-04-01

129

Construction and Verification of Useful Vectors for Ectopic Expression and Suppression of Plant Genes.

Full Text Available

2007-05-01

130

Cell Cycle-dependent Expression of Chitin Synthase Genes in Aspergillus nidulans

Full Text Available

2009-06-01

131

Time-resolved measurement of a self-amplified free-electron laser

Full Text Available

2001-03-01

132

Structural basis of binding of fluorescent, site-specific dansylated amino acids to human serum albumin

We report on a time-resolved measurement of self-amplified spontaneous emission free-electron laser (FEL) pulses. We observed that the spikes in such FEL pulses have an intrinsic positive chirp and the energy chirp in the electron bunch mapped directly into the FEL output. The measurement also provides rich information on the statistics of the FEL pulses.

2003-07-11

133

Quantum computing and probability

Human serum albumin (HSA) has two primary binding sites for drug molecules. These sites selectively bind different dansylated amino acid compounds, which—due to their intrinsic fluorescence—have...Full Text Available

2011-04-01

134

Hyperphosphorylation of the Rotavirus NSP5 Protein Is Independent of Serine 67 or NSP2, and the Intrinsic Insolubility of NSP5 Is Regulated by Cellular Phosphatases

Over the past two decades, quantum computing has become a popular and promising approach to trying to solve computationally difficult problems. Missing in many descriptions of quantum computing is just how probability enters into the process. Here, we discuss some simple examples of how uncertainty and probability enter, and how this and the ideas of quantum computing challenge our interpretations of quantum mechanics. It is found that this uncertainty can lead to intrinsic decoherence, and this raises challenges for error correction. (viewpoint)

2009-11-25

135

Helicoidal instability of a scroll vortex in three-dimensional reaction-diffusion systems

The NSP5 protein is required for viroplasm formation during rotavirus infection and is hyperphosphorylated into 32- to 35-kDa isoforms. Earlier studies reported that NSP5 is not hyperphosphorylated...Full Text Available

2006-02-01

136

Efficient higher-order derivatives of the hypergeometric function.

We study the dynamics of scroll vortices in excitable reaction-diffusion systems analytically and numerically. We demonstrate that intrinsic three-dimensional instability of a straight scroll leads to the formation of helicoidal structures. This behavior originates from the competition between the scroll curvature and unstable core dynamics. We show that the obtained instability persists even beyond the meander core instability of the two-dimensional spiral wave. copyright 1998 The American Physical Society.

1998-10-01

137

Dissecting toxin immunity in virus-infected killer yeast uncovers an intrinsic strategy of self-protection

Various physics applications involve the computation of the standard hypergeometric function {sub 2}F{sub 1} and its derivatives. Because it is not an intrinsic in the common programming languages, automatic differentiation tools will either differentiate through the code that computes {sub 2}F{sub 1} if that code is available or require the user to provide a hand-written derivative code. We present options for the derivative computation in the context of an ionization problem and compare the approach implemented in the Diamant library to standard methods.

2008-01-01

138

Detection and Isolation of Ultrasmall Microorganisms from a 120,000-Year-Old Greenland Glacier Ice Core

Toxin-secreting “killer” yeasts were initially identified >40 years ago in Saccharomyces cerevisiae strains infected with a double-stranded RNA “killer”...Full Text Available

2006-03-07

139

Alignments of multi-quasiparticle bands and seniority-dependent reduced pairing

The abundant microbial population in a 3,043-m-deep Greenland glacier ice core was dominated by ultrasmall cells (<0.1 μm³) that may represent intrinsically small organisms...Full Text Available

2005-12-01

140

A large-acceptance Bragg curve spectrometer with a longitudinal electric field and a segmented anode

Pairing correlations in multi-quasiparticle states in deformed nuclei are expected to be reduced because of blocking. New intrinsic states and their associated rotational bands have been identified allowing comparisons to be made as a function of seniority and between nuclei. The possible effects of the reduced pairing on the collective rotation, and its manifestation in terms of alignment, are discussed. 13 refs., 2 figs.

1994-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

Development of radiological emergency preparedness and biological dosimetry technology

A large-acceptance Bragg curve spectrometer with a longitudinal electron collection field and a segmented anode has been constructed and tested. The effects on the charge resolution of the entrance angle and entrance position of the incident particle have been studied. Simulations have been done in order to isolate the contribution to the overall detector performance of the signal-shaping electronics from that of the intrinsic design of the detector. ((orig.))

1994-07-15

142

An evaluation of the pendulum technique of electron beam therapy

Large-scale field tracer experiments have been conducted on Ulchin and Wolsung nuclear sites for the purpose of validating FADAS and of analyzing the environmental characteristics around the nuclear site. The most influential factor in atmospheric dispersion is the meteorological condition. During the experiment, meteorological data were measured on the release point and the selected positions among sampling points. Once radioactive materials are released to the atmosphere, members of public may be exposed through the environmental media such as air, soil and foods. Therefore, to protect the public, adequate countermeasures should be taken at due time for those exposure pathways. Both processes of justification and optimization are applied to a countermeasure simultaneously for decision-making. The work scope of biological research for the radiation protection had contained the search of biological microanalytic methods for the assessment of health effect by radiation and toxic agents, ...

1999-04-01

143

Selective changes of retroelement expression in human prostate cancer

Electron beam therapy of lesions such as cutaneous metastatic carcinoma and reticulum cell sarcoma is performed via multiple portals for a wide irradiation field. However, the dose distribution in border areas is generally uneven, resulting in hot and cold dose areas depending on the intervals of the portals. We performed electron irradiation by the pendulum technique, especially on curved areas, and evaluated the technique in various respects. Pendulum electron beam irradiation is indicated when the length of the cone must be reduced in order to deliver the target volume near the skin surface as well as to avoid the occurrence of hot spots deep in the body. We prepared a 15cm rotating cone by cutting a 30cm fixed irradiation cone. The modification posed no disadvantages in terms of extent and flatness of the beam, or stability during rotation. After determining the relationship between the rotation speed and the dose, the technique was successfully applied to a patient with adult ...

144

British Library Electronic Table of Contents (United Kingdom)

Retroelements constitute a large part of the human genome. These sequences are mostly silenced in normal cells, but genome-wide DNA hypomethylation in cancers might lead to their re-expression. Whether this re-expression really occurs in human cancers is largely unkown. We therefore investigated expression and DNA methylation of several classes of retroelements in human prostate cancer tissues and cell lines by quantitative reverse transcription-polymerase chain reaction and pyrosequencing, respectively. The most striking finding was strong and generalized increased expression of the HERV-K_22q11.23 provirus in cancers, including de novo expression of a spliced accessory Np9 transcript in some tumors. In parallel, DNA methylation in the long terminal repeat (LTR) decreased. Conversely, HER...

2011-01-01

145

Quercetin-induced downregulation of phospholipase D1 inhibits proliferation and invasion in U87 glioma cells

British Library Electronic Table of Contents (United Kingdom)

Phospholipase D (PLD) has been recognized as a regulator of cell proliferation and tumorigenesis, but little is known about the molecules regulating PLD expression. Thus, the identification of small molecules inhibiting PLD expression would be an important advance in PLD-mediated physiology. Quercetin, a ubiquitous bioactive flavonoid, is known to inhibit proliferation and induce apoptosis in a variety of cancer cells. In the present study, we examined the effect of quercetin on the expression of PLD in U87 glioma cells. Quercetin significantly suppressed the expression of PLD1 at the transcriptional level. Moreover, quercetin abolished the protein expression of PLD1 in a time and dose-dependent manner, as well as inhibited PLD activity. Quercetin suppressed NFkB-induced PLD1 expression vi...

2011-01-01

146

Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1

The complexity of gene expression dynamics revealed by permutation entropy

Earlier we have shown that exogenous expression of HIPPI, a molecular partner of Huntingtin interacting protein HIP-1, induces apoptosis and increases expression of caspases-1, -8 and -10 in HeLa and...Full Text Available

2010-01-01

147

The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.

BackgroundHigh complexity is considered a hallmark of living systems. Here we investigate the complexity of temporal gene expression patterns using the concept of Permutation Entropy...Full Text Available

148

Specific expression profile and prognostic significance of peroxiredoxins in grade II-IV astrocytic brain tumors

Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen...Full Text Available

1994-12-01

149

Site specific effects of anosmia and cloacal gland anesthesia on Fos expression induced in male quail brain by sexual behavior

BackgroundPeroxiredoxins (Prxs) have recently been suggested to have a role in tumorigenesis.MethodsWe studied the expression of Prx I-VI and their...Full Text Available

150

Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts

In rats, expression of the immediate early gene, c-fos observed in the brain following male copulatory behavior relates mostly to the detection of olfactory information originating...Full Text Available

2008-12-01

151

Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates

Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate sex-specific differences at the...Full Text Available

2010-02-23

152

Role of Estrogen Receptor-? in the Regulation of Claudin-6 Expression in Breast Cancer Cells

alpha-actinins are actin microfilament crosslinking proteins. Vertebrate actinins fall into two classes: the broadly-expressed actinins 1 and 4 (actn1 and actn4)...Full Text Available

2009-11-01

153

Robust consensus clustering for identification of expressed genes linked to malignancy of human colorectal carcinoma

PurposeIn our previous studies we showed that upregulating claudin-6 (CLDN6) expression may contribute to preventing breast cancer, and that 17β-estradiol induces a concentration-...Full Text Available

2011-03-01

154

Rhythmic expression of an egr-1 transgene in rats distinguishes two populations of photoreceptor cells in the retinal outer nuclear layer

Previous studies have been conducted in gene expression profiling to identify groups of genes that characterize the colorectal carcinoma disease. Despite the success of previous attempts to identify...Full Text Available

155

Reduced thermal sensitivity and Nav1.8 and TRPV1 channel expression in sensory neurons of aged mice

PurposeNocturnal rhythms of gene expression in the retina are known to be both darkness- and circadian clock-dependent, but their role and cellular location are not well defined....Full Text Available

156

Recent Applications of DNA Microarray Technology to Toxicology and Ecotoxicology

Sensory neurons in aging mammals undergo changes in anatomy, physiology and gene expression that correlate with reduced sensory perception. In this study we compared young and aged mice to identify...Full Text Available

2006-06-01

157

Prostaglandin H₂ synthase-1 and -2 expression in guinea pig gestational tissues during late pregnancy and parturition

Gene expression is a unique way of characterizing how cells and organisms adapt to changes in the external environment. The measurements of gene expression levels upon exposure to a chemical can be...Full Text Available

2006-01-01

158

Prognostic evaluation of metallothionein expression in human colorectal neoplasms.

Increased intrauterine prostaglandin (PG) production is crucial for the initiation of parturition. To investigate the mechanisms controlling intrauterine PG synthesis, we examined the expression of...Full Text Available

2005-12-15

159

Parent-Child Interactions in Relation to Critical and Emotionally Overinvolved Expressed Emotion (EE): Is EE a Proxy for Behavior?

AIM: To investigate the role of metallothionein in colorectal tumours and the possible relation with other factors associated with tumour progression: expression of cathepsin D (CD), CD44, p53, Rb,...Full Text Available

1999-12-01

160

PKC expression is regulated by dietary K intake and mediates internalization of SK channels in the CCD

Expressed emotion measures, encompassing dimensions of criticism (CRIT), and emotional overinvolvement (EOI) are increasingly being used to assess the parent–child relationship in child...Full Text Available

2004-02-01

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161

PET imaging of heat-inducible suicide gene expression in mice bearing head and neck squamous cell carcinoma xenografts

We have used Western blot analysis and immunocytochemistry to determine the effect of dietary K intake on the expression of protein kinase C (PKC) isoforms in the kidney. Western blot has demonstrated...Full Text Available

2004-06-01

162

Neuronatin: A New Inflammation Gene Expressed on the Aortic Endothelium of Diabetic Mice

The ability to achieve tumor selective expression of therapeutic genes is an area that needs improvement for cancer gene therapy to be successful. One approach to address this is through the...Full Text Available

2009-02-01

163

Neoplastic transformation of prostatic and urogenital epithelium by the polyoma virus middle T gene.

OBJECTIVE—Identification of arterial genes and pathways altered in obesity and diabetes.RESEARCH DESIGN AND METHODS—Aortic gene expression profiles of...Full Text Available

2008-10-01

164

NOTCH3 expression is induced in mural cells through an autoregulatory loop that requires endothelial-expressed JAGGED1

Male transgenic mice expressing the polyomavirus middle T (PyV-MT) gene exhibited growth and developmental abnormalities in prostatic and other urogenital epithelium. Expression of PyV-MT was directed...Full Text Available

1996-10-01

165

Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study

Endothelial cells and mural cells (smooth muscle cells, pericytes, or fibroblasts) are known to communicate with one another. Their interactions not only serve to support fully functional blood...Full Text Available

2009-02-27

166

Modulation of Expression of the ToxR Regulon in Vibrio cholerae by a Member of the Two-Component Family of Response Regulators

AimsThe aims of the study were to study the effect of pre-operative treatment on the expression of tumour-related proteins and to correlate the expression of these...Full Text Available

2009-02-01

167

Modulation of Aire regulates the expression of tissue-restricted antigens

The ToxRS system in Vibrio cholerae plays a central role in the modulation of virulence gene expression in response to environmental stimuli. An integration of multiple signalling inputs...Full Text Available

1998-12-01

168

Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines

AbstractIntrathymic expression of tissue-restricted antigens (TRAs) has been viewed as the key element in the induction of central tolerance and recently, a central role for the autoimmune...Full Text Available

2008-01-01

169

Linking Gene Expression and Functional Network Data in Human Heart Failure

BackgroundCD26 (dipeptidyl peptidase IV, DPPIV) is a 110 kDa surface glycoprotein expressed in most normal tissues, and is a potential novel therapeutic target for selected cancers....Full Text Available

170

Leptin receptor gene expression and number in the brain are regulated by leptin level and nutritional status

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

171

Lameness, Activity Time-Budgets, and Estrus Expression in Dairy Cattle

Hormone potency depends on receptor availability, regulated via gene expression and receptor trafficking. To ascertain how central leptin receptors are regulated, the effects of leptin challenge, high-fat...Full Text Available

2009-07-15

172

Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene.

The aim of the present study was to identify specific behavioral patterns that contribute to diminished estrus expression in lame cows. Behavioral scan and focal sampling were used to examine...Full Text Available

2008-12-01

173

Indoleamine 2,3-dioxygenase-expressing leukemic dendritic cells impair a leukemia-specific immune response by inducing potent T regulatory cells

Exploring the possibility of enhancing the properties of baculoviruses as biological control agents of insect pests, we tested the effect of expressing an insect gene (jhe) encoding juvenile hormone...Full Text Available

1992-05-01

174

Increased expression of cholesterol transporter ABCA1 is highly correlated with severity of dementia in AD hippocampus

BackgroundThe immunoregulatory enzyme indoleamine 2,3-dioxygenase, which catalyzes the conversion of tryptophan into kynurenine, is expressed in a significant subset of patients...Full Text Available

2010-12-01

175

Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis

To gain insight into ATP-binding cassette transporter A1 (ABCA1) function and its potential role in AD pathology, we analyzed the expression of the cholesterol transporter ABCA1...Full Text Available

2010-03-08

176

Identification of Achaete-scute complex-like 1 (ASCL1) target genes and evaluation of DKK1 and TPH1 expression in pancreatic endocrine tumours

IntroductionPrevious observations suggest that active systemic juvenile idiopathic arthritis (sJIA) is associated with a prominent erythropoiesis gene-expression signature. The aim...Full Text Available

2010-01-01

177

Identification and expression of differentially expressed genes in the hard clam, Mercenaria mercenaria, in response to quahog parasite unknown (QPX)

BackgroundASCL1 role in pancreatic endocrine tumourigenesis has not been established. Recently it was suggested that ASCL1 negatively controls expression of the...Full Text Available

178

Gene expression analysis of interferon ? in laser capture microdissected cervical epithelium

BackgroundThe hard clam, Mercenaria mercenaria, has been affected by severe mortality episodes associated with the protistan parasite QPX (Quahog Parasite Unknown)...Full Text Available

179

Fusion expression of pedA gene to obtain biologically active pediocin PA-1 in Escherichia coli *

Optimal sample handling techniques for tissue preparation and storage, RNA extraction and quantification, and target gene detection are crucial for reliable gene expression analysis. Methods...Full Text Available

2008-10-01

180

Full genome gene expression analysis of the heat stress response in Drosophila melanogaster

Two heterologous expression systems using thioredoxin (trxA) as a gene fusion part in Escherichia coli were developed to produce recombinant pediocin PA-1. Pediocin...Full Text Available

2011-01-01

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181

Expression of embryonic hemoglobin genes in. cap alpha. -thalassemic and in. beta. -duplication mice

The availability of full genome sequences has allowed the construction of microarrays, with which screening of the full genome for changes in gene expression is possible. This method can provide a wealth...Full Text Available

2005-10-01

182

Expression of calbindin-D28k and its regulation by estrogen in the human endometrium during the menstrual cycle

The results of a study of the expression of embryonic hemoglobin genes in mice which show an imbalance of alpha and non-alpha chain synthesis are reported. (ACR)

1979-01-01

183

Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro.

Human endometrium resists embryo implantation except during the 'window of receptivity'. A change in endometrial gene expression is required for the development of receptivity. Uterine calbindin-D28k...Full Text Available

184

Expression of Fc receptors is suppressed in alveolar macrophages from patients with sarcoidosis.

Infection with the Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial cells. Expression of large T antigen occurred...Full Text Available

1993-04-01

185

Expression and Characterization of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase PknB

To study the expression of Fc receptors in human alveolar macrophages (AM), the cells were collected from 12 healthy controls and 22 patients with sarcoidosis and the activity involved in binding to...Full Text Available

1988-09-01

186

Establishment and expression of cellular polarity in fucoid zygotes.

PknB is a member of the newly discovered eukaryotic-like protein serine/threonine kinase (PSTK) family of proteins. The pknB gene was cloned and expressed in Escherichia coli....Full Text Available

1999-11-01

187

Essential Role of Phox2b-Expressing Ventrolateral Brainstem Neurons in the Chemosensory Control of Inspiration and Expiration

Zygotes of fucoid algae have long been studied as a paradigm for cell polarity. Polarity is established early in the first cell cycle and is then expressed as localized growth and invariant cell division....Full Text Available

1992-06-01

188

Endogenous expression of Hras^G12V induces developmental defects and neoplasms with copy number imbalances of the oncogene

Phox2b-expressing neurons of the retrotrapezoid nucleus (RTN) located in the ventrolateral brainstem are sensitive to changes in PCO₂/pH, have excitatory projections...Full Text Available

2010-09-15

189

Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii?

We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms of tumor initiation. They had high perinatal mortality, abnormal...Full Text Available

2009-05-12

190

Differential expression of prostaglandin-H synthase isoenzymes in normal and activated keratinocytes in vivo and in vitro.

Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR....Full Text Available

2009-06-01

191

Differential Regulation of PDE5 Expression in Left and Right Ventricles of Feline Hypertrophy Models

Normal mouse epidermis constitutively expresses prostaglandin-H synthase 1 (PGHS-1) but no PGHS-2. Acute inflammation and epidermal hyperplasia, (hyperplastic transformation), as evoked in adult mouse...Full Text Available

1995-07-01

192

Dexamethasone and nitric oxide synthase gene expression in brain.

BackgroundThough long known to affect smooth muscle biology, recent studies indicate that phosphodiesterase 5 (PDE5) is also expressed in myocardium. Recognizing that the regulation...Full Text Available

193

Development and evaluation of a virtual microscopy application for automated assessment of Ki-67 expression in breast cancer

Systemic administration of lipopolysaccharide (LPS), which causes endotoxemia and systemic inflammation, has been reported to induce expression of the gene for type II inducible nitric oxide synthase...Full Text Available

1997-03-01

194

Defective major histocompatibility complex class I expression on lymphoid cells in autoimmunity.

BackgroundThe aim of the study was to develop a virtual microscopy enabled method for assessment of Ki-67 expression and to study the prognostic value of the automated analysis in...Full Text Available

195

DIFFERENTIAL EXPRESSION OF HPA AXIS HOMOLOG IN THE SKIN

Lymphocytes from patients with insulin-dependent diabetes mellitus (IDDM), a chronic autoimmune disease, have recently been shown to have decreased surface expression of MHC class I antigens. Since...Full Text Available

1993-05-01

196

Coordination of PAD4 and HDAC2 in the regulation of p53 target gene expression

SummaryHuman skin expresses elements of the hypothalamo-pituitary-adrenal (HPA) axis including pro-opiomelanocortin (POMC), corticotropin releasing hormone (CRH), the CRH receptor-1...Full Text Available

2007-02-01

197

Cloning and expression of recombinant, functional ricin B chain.

Histone Arg methylation and Lys acetylation have been found to cooperatively regulate the expression of p53 target genes. Peptidylarginine deiminase 4 (PAD4) is an enzyme that citrullinates...Full Text Available

2010-05-27

198

Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues

The cDNA encoding the B chain of the plant toxin ricin has been cloned and expressed in monkey kidney COS-M6 cells. The recombinant B chain was detected by labeling the transfected cells with [35S]methionine...Full Text Available

1987-08-01

199

Characterisation of the Expression of NMDA Receptors in Human Astrocytes

AIM: To investigate the relation of Fas and Fas ligand (FasL) protein expression with carcinogenesis and metastasis of gastric carcinoma.METHODS: Immunohistochemistry was used to detect Fas and...Full Text Available

2010-03-14

200

Characterisation of CYP3A gene subfamily expression in human gastrointestinal tissues.

Astrocytes have long been perceived only as structural and supporting cells within the central nervous system (CNS). However, the discovery that these glial cells may potentially express receptors capable...Full Text Available

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201

Biotin binders selected from a random peptide library expressed on phage.

The human CYP3A subfamily is of interest due to its multiplicity, activity toward known carcinogens, and extrahepatic expression. In situ hybridisation analysis of formalin fixed, routinely processed...Full Text Available

1995-02-01

202

Antisense imaging of gene expression in the brain in vivo

Recombinant biotin-binding phages were affinity-selected from a random peptide library expressed on the surface of filamentous phage. Phage binding to biotinylated proteins was half-maximally inhibited...Full Text Available

1993-08-01

203

A supervised approach for predicting patient survival with gene expression data

Antisense radiopharmaceuticals could be used to image gene expression in the brain in vivo, should these polar molecules be made transportable through the blood–brain barrier....Full Text Available

2000-12-19

204

A highly conserved regulatory element controls hematopoietic expression of GATA-2 in zebrafish

Rapid development in genomics in recent years has allowed the simultaneous measurement of the expression levels of thousands of genes using DNA microarrays. This has offered tremendous potential...Full Text Available

2010-01-01

205

A Predictive Risk Probability Approach for Microarray Data with Survival as an Endpoint

BackgroundGATA-2 is a transcription factor required for hematopoietic stem cell survival as well as for neuronal development in vertebrates. It has been shown that specific expression...Full Text Available

206

Update of the Navy Contract Writing Guide

Gene expression profiling has played an important role in cancer risk classification and has shown promising results. Since gene expression profiling often involves determination of a set of...Full Text Available

2008-01-01

207

Triptolide-induced suppression of phospholipase D expression inhibits proliferation of MDA-MB-231 breast cancer cells.

... These requirements may be expressed in terms of MIL-STD-2073-1, DoD Material Procedures for Development and Application of Packaging ...

2004-06-01

208

Transfection of endothelial cells with EJ-ras oncogene affects cell cycle and the expression of hyaluronic acid, syndecan-4, epimerase and sulfotransferases

Full Text Available

2009-09-01

209

The Measurement of Air-Sea Fluxes

No abstract prepared.

2005-07-01

210

Regulation of the expression of the apolipoprotein(a) gene : Evidence for a regulatory role of the 5' distal ACR enhancer in YAC transgenic mice

... The micrometeorological community often expresses these relations in resistance law terms (Garratt and Hicks, 1973; Hicks et al., 1985) ...

1990-10-09

211

Feynman-Wiener path integral representation for scalar advected diffusion

No abstract prepared.

2003-01-30

212

Effect of Resveratrol on the Induction of Cdk Inhibitor p21 and Pro-apoptotic Bax Expression by amyloid-? in Astroglioma C6 Cells

We write a path-integral expression for the Green function of a advected scalar on a fluid flux. (author)

2000-07-01

213

Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.

Full Text Available

2005-04-01

214

Prolactin and Its Receptor Are Expressed in Murine Hair Follicle Epithelium, Show Hair Cycle-Dependent Expression, and Induce Catagen

We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1...Full Text Available

1993-01-01

215

High-cell Density Shake-flask Expression and Rapid Purification of the Large Fragment of Thermus aquaticus DNA Polymerase I Using a New Chemically and Temperature Inducible Expression Plasmid in Escherichia coli*

Here, we provide the first study of prolactin (PRL) and prolactin receptor (PRLR) expression during the nonseasonal murine hair cycle, which is, in contrast to sheep, comparable with the human scalp...Full Text Available

2003-05-01

216

The Significance of PITX2 Overexpression in Human Colorectal Cancer

We have developed a new expression vector, pcI^ts ind⁺, based upon the powerful rightward promoter of bacteriophage lambda, which is controlled by a temperature-sensitive...Full Text Available

2009-02-01

217

British Library Electronic Table of Contents (United Kingdom)

Purpose The paired-like homeodomain transcription factor 2 (PITX2) gene encodes a transcription factor controlled by the WNT/Dvl/CTNNB1 and Hedgehog/TGFB pathways in the pathogenesis of colorectal cancer (CRC). Although PITX2 is reportedly involved in various functions, including tissue development by controlling cell growth, its significance in CRC remains unclear. We report our findings regarding abnormal PITX2 expression in human CRC. Methods PITX2 expression was evaluated in 5 human CRC cell lines and 92 primary CRC samples. Cell growth was evaluated after inhibition of PITX2 expression or after exogenous introduction of PITX2. Results PITX2 expression was seen in all the five CRC cell lines. The study of tissue samples indicated that PITX2 expression was significantly higher in cancer...

2011-01-01

218

Why there is a field algebra with a compact gauge group describing the superselection structure in particle physics

The XMM-Newton wide-field survey in the COSMOS field. IV: X-ray spectral properties of Active Galactic Nuclei

Given the local observables in the vacuum sector fulfilling a few basic principles of local quantum theory, we show that the superselection structure, intrinsically determined a priori, can always be described by a unique compact global gauge group acting on a field algebra generated by field operators which commute or anticommute at spacelike separations. The field algebra and the gauge group are constructed simultaneously from the local observables. There will be sectors obeying parastatistics, and intrinsic notion derived from the observables, if and only if the gauge group is non-Abelian. Topological charges would manifest themselves in field operators associated with spacelike cones but not localizable in bounded regions of Minkowski space. No assumption on the particle spectrum or even on the covariance of the theory is made. However the notion of superselection sector is tailored to theories without massless particles. When translation ...

1990-01-01

219

Magnetic moments of C isotopes studied with antisymmetrized molecular dynamics

We present a detailed spectral analysis of point-like X-ray sources in the XMM-COSMOS field. Our sample of 135 sources only includes those that have more than 100 net counts in the 0.3-10 keV energy band and have been identified through optical spectroscopy. The majority of the sources are well described by a simple power-law model with either no absorption (76%) or a significant intrinsic, absorbing column (20%).As expected, the distribution of intrinsic absorbing column densities is markedly different between AGN with or without broad optical emission lines. We find within our sample four Type-2 QSOs candidates (L_X > 10^44 erg/s, N_H > 10^22 cm^-2), with a spectral energy distribution well reproduced by a composite Seyfert-2 spectrum, that demonstrates the strength of the wide field XMM/COSMOS survey to detect these rare and underrepresented sources.

2006-01-01

220

Effect of oxide treatment at the microcrystalline tunnel junction of a-Si:H/a-Si:H tandem cells

We studied the magnetic dipole moments #mu# of even-odd C isotopes, ranging from proton-rich to neutron-rich nuclei, with antisymmetrized molecular dynamics (AMD). The results are in good agreement with the experimental data. In the "9C ground state the total intrinsic spin of the protons is found to be nonzero (S_p#not =#0), which is unusual in even-odd nuclei. The interesting point is that the spin-orbit force breaks slightly the coupling off of intrinsic spins of the even nucleon group in isospin T=3/2 nuclei. This result is consistent with the newly measured #mu# data that, when combined with "9Li data, indicate an unusual left-angle #sigma# right-angle value larger than unity. A #mu# moment -1.05#mu#_N of "1"7C is theoretically predicted. We also show a good reproduction of E2 transition data. copyright 1996 The American Physical Society.

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221

Distances of Quasars and Quasar-Like Galaxies: Further Evidence that QSOs may be Ejected from Active Galaxies

The electrical transport taking place in the {mu}c-Si tunnel recombination junction (TRJ) of a-Si:H/a-Si:H tandem solar cells and the role of CO{sub 2} plasma oxidation performed between microcrystalline layers is investigated in this paper with the computer code AMPS. Oxidized interfaces were modelled as simple highly defective intrinsic {mu}c-Si layers. Two different tunnel junction structures are studied in this paper: (a) (n){mu}c-Si/oxide/(p){mu}c-Si and (b) (n){mu}c-Si/(i){mu}c-Si/(p){mu}c-Si. In the last configuration the oxide interface is removed and replaced by a thin defective (i) {mu}c-Si layer. Both tunnel junctions have comparable theoretical and experimental tandem solar cell efficiencies which indirectly proves that our modelling assumption for oxidised interfaces is correct. A-Si:H/a-Si:H tandem solar cell efficiencies depend on the thickness of the intrinsic layer introduced in the tunnel junction. The optimisation of this ...

2000-05-01

222

AP Theory IV: Intrinsic Topological Quantum Langlands Theory

If high-redshift QSOs are ejected from the nuclei of low-redshift galaxies, as some have claimed, a large portion of their redshift must be intrinsic (non-Doppler). If these intrinsic components have preferred values, redshifts will tend to cluster around these preferred values and produce peaks in the redshift distribution. Doppler ejection and Hubble flow components will broaden each peak. Because ejection velocities are randomly directed and Hubble flow components are always positive, in this model all peaks are expected to show an asymmetry, extending further out in the red wing. If peaks are present showing this predicted asymmetry, it can lead directly to an estimate of quasar distances. Using two quasar samples, one with high redshifts and one with low, it is shown here that not only do all peaks in these two redshift distributions occur at previously predicted preferred values, they also all show the predicted extra extension in the red ...

2004-01-01

223

Trends in Neuroendocrinology

Without using any moduli, sheaves, stacks, nor any analytic, nor category-type arguments, we exhibit an analogue to Geometric Langlands Theory in an entirely model-independent, non-perturbative,purely smooth topological context in Artin Presentation Theory. A basic initial feature is that AP Theory, as a whole, is already, ab initio, a universal canonical 2D sigma-model, targeting smooth, compact, simply-connected 4-manifolds with a connected boundary, and its topological Planckian quantum starting point, as well as its cone-like, infinitely-generated at each stage, graded group of homology-preserving, but topology-changing transitions/interactions, exhibit the most general qualitative S-duality. We first point out the numerous mathematically rigorous, model-free, (i.e., intrinsic), topological AP analogues with the heuristic Kapustin-Witten version of Geometric Langlands theory, as well as the crucial differences between the two theories. The latter have to exist ...

2010-01-01

224

British Library Electronic Table of Contents (United Kingdom)

The mammalian circadian timing system is organized into hierarchical structures with a central clock in the suprachiasmatic nucleus (SCN) and subsidiary peripheral oscillators. After the discovery of the local clockwork in peripheral organs and tissues, which have a molecular makeup similar to the central pacemaker SCN, uncovering the roles of the peripheral clock in the rhythmic physiology has been an emerging goal in chronobiology. Glucocorticoid (GC) is a multifunctional adrenal steroid hormone that shows a robust circadian rhythm. The daily GC rhythm has long been thought to be governed by the SCN via the hypothalamus-pituitary-adrenal neuroendocrine axis. Recent findings, however, indicate that multiple regulatory mechanisms, including the adrenal intrinsic mechanism by the peripheral...

2011-01-01

225

Towards a computational theory of experience

British Library Electronic Table of Contents (United Kingdom)

A standing challenge for the science of mind is to account for the datum that every mind faces in the most immediate - that is, unmediated - fashion: its phenomenal experience. The complementary tasks of explaining what it means for a system to give rise to experience and what constitutes the content of experience (qualia) in computational terms are particularly challenging, given the multiple realizability of computation. In this paper, we identify a set of conditions that a computational theory must satisfy for it to constitute not just a sufficient but a necessary, and therefore naturalistic and intrinsic, explanation of qualia. We show that a common assumption behind many neurocomputational theories of the mind, according to which mind states can be formalized solely in terms of instan...

2011-01-01

226

Solution of the orthopositronium lifetime puzzle

The intrinsic decay rate of orthopositronium formed in SiO/sub 2/ powder is measured using the direct 2 gamma correction method such that the time dependence of the pick-off annihilation rate is precisely determined. The decay rate of orthopositronium is found to be 7.0396+or-0.0012(stat.)+or-0.0011(sys.)/ mu s/sup -1/, which is consistent with our previous measurements with about twice the accuracy. Results agree well with the O( alpha /sup 2/) QED prediction, and also with a result reported very recently using nanoporous film.

2004-01-01

227

Radiation-stimulated diffusion of aerosols

we report an ultrafast synchrotron x-ray phase contrast imaging study of the primary breakup mechanism of a coaxial air-assisted water jet. We demonstrate that there exist great similarities in the phenomenology of primary breakup with that of the secondary breakup. Especially, a membrane-mediated breakup mechanism dominates the breakup process for a wide range of air speeds. This finding reveals the intrinsic connections of these two breakup regimes and has deep implications on the unified theoretical approach in treating the breakup mechanism of high speed liquid jet.

2007-01-01

228

On the fatigue threshold behaviour of two ferrite-pearlite microalloyed steels

The diffusion coefficient of particles in radioactive gases has been calculated with account of random wandering of aerosols (occurrence of local fields affecting the particles; recoils accompanying radiation emitted by particles, etc.). To determine the diffusion coefficient, the method of Fokker-Planck equation derivation was used. A formula is presented for calculating the radiation-stimulated diffusion coefficient. A linear growth of the diffusion coefficient with radioactivity is noted according to the formula, the diffusion coefficient is mainly determined by the field in the radiation damage region. The aerosol radioactivity may result in a more rapid deposition of aerosols in the pipelines and aerosol purification systems. The diffusion rate grows not only in the presence of intrinsic radioactivity but in case of external radiation exposure as well.

1984-04-01

229

Nondestructive analysis for "2"3"2U and decay progeny in animal tissues

Long crack fatigue threshold ({delta} K{sub th}) and crack closure behaviour of two thermomechanically processed microalloyed (MA) ferrite-pearlite steels are reported. {delta} K{sub th} and crack closure were measured using a dynamic compliance measurement technique. Crack closure was observed at low load ratios (R < 0.5) and the fatigue thresholds decreased with increasing load ratio. In both steels, the closure-free intrinsic threshold ({delta} K{sub eff} {sub th}) was found to be independent of the load ratio. (orig.)

2000-07-01

230

Nanocrystalline permanent magnets with enhanced properties

Direct determination of "2"3"2U and its decay products in animal tissues appears to be feasible using an intrinsic Ge(Li) diode detector (for energies of 5-100 keV) and a NaI(Tl) anticoincidence-shielded Ge(Li) diode for higher-energy gamma photons. The detection sensitivity for "2"3"2U and "2"2"8Th is 0.03 and 0.01 nCi, respectively, using a 300-min counting time.

1977-05-01

231

Local lattice structure, crystal field and energy level patterns in CsCdBr_3:Tm"3"+ crystals

Parameters of permanent magnets result from the combination of intrinsic properties such as saturation magnetization, magnetic exchange, and magnetocrystalline energy, as well as microstructural parameters such as phase structure, grain size, and orientation. Reduction of grain size into nanocrystalline regime (#approx# 50 nm) leads to the enhanced remanence which derives from ferromagnetic exchange coupling between highly refined grains. In this study the fundamental phenomena, quantities, and structure parameters, which define nanophase permanent magnets are presented and discussed. The theoretical considerations are confronted with experimental data for nanocrystalline Sm-Fe-N type permanent magnets. (author)

2001-09-23

232

Level density parameter and fission probability calculations in heavy-ion-induced fission reactions

In CsCdBr_3, Tm"3"+ substitutes for Cd"2"+. It predominately forms symmetric dimer centers and single-ion centers, both of trigonal symmetry. The energy level schemes of both centers were determined by EPR and site-selective laser spectroscopy. To describe the spectra term dependent crystal-field parameters were deduced on the basis of a microscopic model taking into account the local lattice deformation induced by the impurity centers and the quasi-resonant virtual scattering of intrinsic lattice excitations by the Tm"3"+ ions. (orig.)

1998-07-24

233

Ionizing Radiation and Life

Based on the single-particle levels given by Nilsson, the intrinsic and effective (with collective effects) level density parameters as a function of the excitation energy for the "1"8"6Os, "1'8"7Ir, "1"8"9Os and "1"9"3Au deformation nuclei have been calculated in the range of the excitation energy up to 150 MeV. The calculated fission probabilities P_f (U) are consistent satisfactorily with the experimental data when a nonadiabatic estimation of the collective effects was used to calculated the nuclear level density parameters.

234

British Library Electronic Table of Contents (United Kingdom)

Abstract Ionizing radiation is a ubiquitous feature of the Cosmos, from exogenous cosmic rays (CR) to the intrinsic mineral radioactivity of a habitable world, and its influences on the emergence and persistence of life are wide-ranging and profound. Much attention has already been focused on the deleterious effects of ionizing radiation on organisms and the complex molecules of life, but ionizing radiation also performs many crucial functions in the generation of habitable planetary environments and the origins of life. This review surveys the role of CR and mineral radioactivity in star formation, generation of biogenic elements, and the synthesis of organic molecules and driving of prebiotic chemistry. Another major theme is the multiple layers of shielding of planetary surfaces from th...

2011-01-01

235

Instanton contributions to the $\\tau$ decay widths

Inner-shell excitation of intrinsic luminescence and resonantly excited X-ray fluorescence at Be 1s edge in oriented BeO crystals

Contrary to some previous claims, we find a sizable instanton contribution to the finite energy sum rule used to extract the value of the strong coupling from the measured $\\tau$ decay widths. It is of the same order of magnitude as standard nonperturbative corrections induced by vacuum quark and gluon condensates. Our result indicates that there might be no hierarchy of power corrections in finite energy sum rules at the scale of $\\tau$ mass. Therefore, the standard nonperturbative corrections do not necessarily improve the accuracy of the theoretical predicition, but can rather be used to estimate an intrinsic accuracy of the pure perturbative calculation, which turns out to be rather high on this evidence, of order one percent.

1993-01-01

236

Incremental learning for recognizing handwritten characters using neural networks

The results of studies of the time-resolved luminescence of self-trapped excitons (STE) with different multiplicity as well as the X-ray emission in oriented BeO crystals under excitation in the vicinity of the 1 s Be photoabsorption edge are presented. The branching of the electronic excitations relaxation depends strongly on the crystal orientation after the inner-shell excitation. The common features between the processes of the STE luminescence and resonantly excited X-ray fluorescence have been discussed.

2007-05-21

237

Generator coordinate method for triaxial quadrupole collective dynamics in strontium isotopes

Artificial Neural Networks (ANNs) are parallel distributed processing machines. The unique characteristics of ANNs are: Fault tolerance, robustness, plasticity and generalization. These offer great potential in many AI applications such as character recognition. Handwritten character recognition is an intrinsically interesting problem, but the difficulties of this task are the many variations in the characters. A robust new incremental learning method, which combines supervised and unsupervised learning paradigms implemented by the Functional Link Net, is illustrated with experimental results. Clustering, based on unsupervised learning, classifies the input data into several categories. The supervised learning paradigm then further classifies the data in the clustered categories.

1989-01-01

238

Free electron laser seeded by ir laser driven high-order harmonic generation

We discuss the algebraic structure of the generator coordinate method for triaxial quadrupole collective motion. The collective solutions are classified according to the representations of the permutation group of the intrinsic axes. Our method amounts to an approximate angular-momentum projection. We apply it to a study of the spherical-to-deformed-shape transition in light even strontium isotopes {sup 78-88}Sr. We find that triaxial configurations play a significant role in explaining the structure of the transitional isotopes {sup 80-82}Sr. (orig.).

1991-07-29

239

Feasibility study on the development of proton accelerator

Coherent x-ray production by a seeded free electron laser (FEL) is important for next generation synchrotron light sources. The authors examine the feasibility and features of FEL emission seeded by a high-order harmonic generation (HHG) of an infrared laser. In addition to the intrinsic FEL chirp, the longitudinal profile and spectral bandwidth of the HHG seed are modified significantly by the FEL interaction well before saturation. This smears out the original attosecond pulselet structure. The authors introduce criteria for this smearing effect on the pulselet and the stretching effect on the entire pulse. They discuss the noise issue in such a seeded FEL.

2007-01-08

240

Estimative of relative stiffness of the exudate gum polysaccharides

A feasibility on the development of a high energy proton accelerator to be used for R and D in the nuclear field of korea was studied. The proposed one is a proton linac with parameters of about 1 GeV, 20 mA which can supply enough neutrons by the spallation reaction to drive a subcritical reactor. It= is expected to solve the intrinsic problem in the nuclear field such as safety, nuclear waste, proliferation and resource. The study was carried out through a multi-institutional cooperation of universities, institute and industry for a national consensus. 5 refs., 8 tabs., 8 figs. (author)

1996-10-01

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241

Are classical tachyons slower-than-light quantum particles

The Smidsrod empirical stiffness parameter (B) of A. occidentale and A. lebbeck gum were determined using the correlation of intrinsic viscosity [#eta#] with ionic strength. The B value of 0.204 and 0.193 found respectively for A. occidentale and A. lebbeck suggests a flexible the molecule. The ionic strength has a greater influence on the [#eta#]. The decrease of [#eta#] increase of I, from 0.01 M to 0.1 M of NaCl, is higher for A. lebbeck (89%) than for A. occidentale (19%). (author)

2001-11-11

242

Accelerating Nearest Neighbor Search on Manycore Systems

After having studied the shape that a tachyon T (e.g., intrinsically spherical) would take up, we show in an explicit example that the characteristics of classical tachyons are similar to those of the ordinary (slower-than-light) quantum particles. In particular, a realistic tachyon is associated with a ''phase speed'' V(V/sup 2/>c/sup 2/), but with a ''group speed'' upsilon=c/sup 2//V (upsilon/sup 2/

1983-07-02

243

Cyclin-Dependent Kinase Inhibitor p16 and p21 Expression, and Cell Cycle Change in Human Lens Epithelial Cell Line SRA 01/04 following Contact Inhibition in Normal Culture

We develop methods for accelerating metric similarity search that are effective on modern hardware. Our algorithms factor into easily parallelizable components, making them simple to deploy and efficient on multicore CPUs and GPUs. Despite the simple structure of our algorithms, their search performance is provably sublinear in the size of the database, with a factor dependent only on its intrinsic dimensionality. We demonstrate that our methods provide substantial speedups on a range of datasets and hardware platforms. In particular, we present results on a 48-core server machine, on graphics hardware, and on a multicore desktop.

2011-01-01

244

British Library Electronic Table of Contents (United Kingdom)

Abstract Purpose: To describe the pattern of expression of the cyclin-dependent kinase inhibitors (CDKIs) p16, p21 and p27, and the cell cycle in SRA 01/04 cells relative to contact inhibition. Methods: SRA 01/04 cells were grown to overconfluence under normal conditions. At various phases of the cell growth, cells were assayed by flow cytometry and Western blotting for the expression of CDKIs. Results: Expression of p16 was detected from early logarithmic growth to stationary phases, during which the number of cells in G0/G1 increased from 46 to 69%. Expression of p21 was detected only during the overgrowth phase, when 60% of the cells were in G0/G1. Expression of p27 was not observed in SRA 01/04 cells. Conclusions: p16 expression was likely mediated by G0/G1 arrest to induce contact inh...

2011-01-01

245

Intrinsic Dosimetry Of Glass Containers Used To Transport Nuclear Materials: Potential Implications to the Field of Nuclear Forensics

Total skin electron beam therapy followed by adjuvant psoralen/ultraviolet-a light in the management of patients with T1 and T2 cutaneous T-cell lymphoma (mycosis fungoides)

Thermoluminescence (TL) and Electron Paramagnetic Resonance (EPR) dosimetry were used to measure dose effects in borosilicate glass with time, from 10 minutes to #approx#60 days following exposure to a dose of up to 10,000 Rad. TL and EPR results were consistent and performed similarly, with both techniques capable of achieving an estimated limit of detection of between 50-100 Rad. Three peaks were identified in the TL glow curve at roughly 110 C, 205 C, and 225 C. The intensity of the 205 C peak was the dominant peak over the time period of this study. The stability of all of the peaks with time since irradiation increased with their corresponding temperature and little or no variation was observed in the glow curve response to a specified total dose attained at different dose rates. The intensity of the 205 C peak decreased logarithmically with time regardless of total dose. Based upon a conservative limit of detection of 330 Rad, a 10,000 Rad dose would still be detected 2.7E3 years ...

246

Expression of fully functional tetrameric human hemoglobin in Escherichia coli

Purpose: Patients with mycosis fungoides [cutaneous T-cell lymphoma (CTCL)] may benefit from adjuvant therapy after completing total skin electron beam therapy (TSEBT). We report the results for (T1(T2)) CTCL patients treated with adjuvant oral psoralen plus ultraviolet light (PUVA) with respect to overall survival (OS), disease-free survival (DFS), salvage of recurrence, and toxicity. Methods and Materials: Between 1974 and 1993, TSEBT was administered to a total of 213 patients with CTCL. Records were reviewed retrospectively, and a total of 114 patients were identified as having T1 or T2 disease. Radiotherapy was provided via a 6-MeV linac to a total of 36 Gy, 1 Gy/day, 4 days/week, for 9 weeks. Beginning in 1988, patients were offered adjuvant PUVA within 2 months of completing TSEBT. This was started at 0.5-2 J/m"2, 1-2 treatments/week, with a taper over 3-6 months. Therapy then continued once per month. There were 39 T1 and 75 T2 patients. Six T1 (15%) and ...

1997-07-15

247

Ectopic expression of Cux1 is associated with reduced p27 expression and increased apoptosis during late stage cyst progression upon inactivation of Pkd1 in collecting ducts

Synthesis genes encoding the human #alpha#- and #beta#-globin polypeptides have been expressed from a single operon in Escherichia coli. The #alpha#- and #beta#-globin polypeptides associate into soluble tetramers, incorporate heme, and accumulate to >5% of the total cellular protein. Purified recombinant hemoglobin has the correct stoichiometry of #alpha#- and #beta#-globin chains and contains a full complement of heme. Each globin chain also contains an additional methionine as an extension to the amino terminus. The recombinant hemoglobin has a C_4 reversed-phase HPLC profile essentially identical to that of human hemoglobin A_0 and comigrates with hemoglobin A_0 on SDS/PAGE. The visible spectrum and oxygen affinity are similar to that of native human hemoglobin A_0. The authors have also expressed the #alpha#- and #beta#-globin genes separately and found that the expression of the #alpha#-globin gene alone results in ...

248

British Library Electronic Table of Contents (United Kingdom)

Abstract Polycystic kidney diseases (PKD) are inherited disorders characterized by fluid-filled cysts primarily in the kidneys. We previously reported differences between the expression of Cux1, p21, and p27 in the cpk and Pkd1 null mouse models of PKD. Embryonic lethality of Pkd1 null mice limits its study to early stages of kidney development. Therefore, we examined mice with a collecting duct specific deletion in the Pkd1 gene. Cux1 was ectopically expressed in the cyst lining epithelial cells of newborn, P7 and P15 Pkd1CD mice. Cux1 expression correlated with cell proliferation in early stages of cystogenesis, however, as the disease progressed, fewer cyst lining cells showed increased cell proliferation. Rather, Cux1 expression in late stage cystogenesis was associated with increased ...

2011-01-01

249

Spin-Lattice Relaxation in Metal-Organic Platinum; 3, Complexes

On the Expressive Power of Polyadic Synchronisation in ?- calculus

The dynamics of spin-lattice relaxation (slr) of metal-organic Pt(II) compounds is studied. Often, such systems are characterized by pronounced zero-field splittings (zfs) of the lowest-lying triplets. Previous expressions for the Orbach slr process do not allow to treat such splitting patterns properly. We discuss the behavior of a modified Orbach expression for a model system and present results of a fit of the temperature dependence of the spin-lattice relaxation rate of Pt(2-thpy)$_2$ based on the modified expression.

1999-01-01

250

Analytic description of X-ray fluorescence intensity as a function of the particle size in powder samples and pulp upon X-ray fluorescence analysis

We extend the pi-calculus with polyadic synchronisation, a generalisation of the communication mechanism which allows channel names to be composite. We show that this operator embeds nicely in the theory of pi-calculus, we suggest that it permits divergence-free encodings of distributed calculi, and we show that a limited form of polyadic synchronisation can be encoded weakly in pi-calculus. After showing that matching cannot be derived in pi-calculus, we compare the expressivity of polyadic synchronisation, mixed choice and matching. In particular we show that the degree of synchronisation of a language increases its expressive power by means of a separation result in the style of Palamidessi's result for mixed choice.

2002-01-01

251

Validation of immune function testing during a 4-week oral toxicity study with FK506.

Analytic expression is derived for X-ray fluorescence dependence on the particle size of powder and slurry media. The expression is obtained for a model of powder medium containing particles of different size and random distribution in the bulk of the sample. Limiting cases of homogeneous and slurry-like media are considered and expressions for calculation of the fluorescence intensity are derived. The results reasonably match the experiment for binary powder mixtures and thus provide evaluation of the powder particle size on the fluorescence intensity for multicomponent polydisperse powder medium

2007-11-01

252

Photoaffinity labeling of ATP and NAD"+ binding sites on recombinant human interleukin 2

Assessment of the immune system's capability to respond to antigens with the generation of specific antibodies, whilst under the influence of a test article, is required in toxicity tests according to the European guideline for repeated dose toxicity testing of medicinal products. The purpose of this study in rats was to validate methodology for the determination of Keyhole Limpet Haemocyanin (KLH)-specific antibodies under the influence of an immunologically active compound. The immunosuppressant FK506, commercially available as Prograf, was administered orally (gavage) to five rats per sex per group at dosages of 0.5mg/kg per day or 3mg/kg per day, for a period of 4 weeks. On days 14 and 22, KLH was administered subcutaneously, with an adjuvant (AluGel), to the two treated groups and a control (i.e. without FK506 treatment) approximately 1h following administration of FK506. Terminal investigations included haematology parameters, titration of KLH-specific antibodies in serum ...

2004-04-01

253

Inhibitor of DNA synthesis is present in normal chicken serum

Interleukin 2 (IL-2) is a T-cell-derived lymphokine critical in the activation and proliferation of T cells, B cells, and lymphokine-activated killer cells. It is a glycoprotein of #approx#15,500 daltons that is synthesized and secreted after activation by antigen or mitogen. By using the analogs 8-azidoadensoine 5'-[#gamma#-"3"2P]triphosphate ([#gamma#-"3"2P]8N_3ATP) and nicotinamide 2-azidoadenine [adenylate-"3"2P]dinucleotide ([#alpha#-"3"2P]2N_3NAD"+) as photoaffinity probes, the authors have detected specific, metal ion-requiring nucleotide binding sites on recombinant human IL-2 (rhIL-2). The specificity of these nucleotide interactions with rhIL-2 was demonstrated by saturation effects and by competition by the parent nucleotides at physiologically relevant concentrations. Saturation of photoinsertion into rhIL-2 occurred at 50 #mu#M [#gamma#-"3"2P]8N_3ATP. Saturation of photoinsertion with [#alpha#-"3"2P]2N_3NAD"+ was observed at 180 #mu#M. The extent of ...

254

Host resistance to rat cytomegalovirus (RCMV) and immune function in adult PVG rats fed herring from the contaminated Baltic Sea

The authors have found that heat-inactivated serum (57/sup 0/C for 1 hour) from normal chickens reduces the proliferation of mitogen-stimulated chicken and murine splenocytes as well as some transformed mammalian lymphoblastoid cell lines. Greater than a 50% reduction in /sup 3/H-thymidine incorporation was observed when concanavalin A (Con A)-activated chicken splenocytes that were cultured in the presence of 10% autologous or heterologous serum were compared to mitogen-stimulated cells cultured in the absence of serum. Normal chicken serum (10%) also caused greater than 95% suppression of /sup 3/H-thymidine incorporation by bovine (EBL-1 and BL-3) and gibbon ape (MLA 144) transformed lymphoblastoid cell lines. The only cell line tested that was not inhibited by chicken serum was an IL-2-dependent, murine cell line. Chicken serum also inhibited both /sup 3/H-thymidine incorporation and IL-2 synthesis by Con A-activated murine splenocytes. Suppression was caused by actions other than ...

1986-03-05

255

Variability in Melanoma Metalloproteinase Expression Profiling

We carried out a feeding study in rats aimed at extending our aboservations of contaminant-induced immunosuppression in harbour seals. Two herring batches were freeze-dried, supplemented and fed to female adult PVG rats for a period of 4 1/2 months. Daily contaminant intakes of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalents (TEQ) were estimated to be 0.3 ng/kg body weight and 1.6 ng/kg in the Atlantic and Baltic groups, respectively. At the end of the feeding experiment, no contaminant-related changes in spleen CD{sub 4}{sup +}/CD{sub 8}{sup +} cellularity, natural killer cell activity, or mitogen-induced proliferative responses of thymus or spleen cells could be detected. However, total thymocyte numbers and thymus CD{sub 4}{sup +}/CD{sub 8}{sup +} ratios were reduced in the Baltic group. A novel model was established to assess the specific T-cell response to rat cytomegalovirus (RCMV). When applied to the feeding study, no differences between the ...

1996-08-01

256

Unmasking Stem/Progenitor Cell Properties in Differentiated ...

The proteolytic activities of a disintegrin and metalloproteinase (ADAM); a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), and matrix metalloproteinase (MMP) families play important...Full Text Available

2010-12-01

257

Unified Analysis of the Average Gaussian Error Probability for a Class of Fading Channels

... In addition to changes in BrdU, we also observed transient changes in p63 gene expression in the myoepithelial/stem cell layer. ...

2007-08-01

258

Tumor necrosis factor-? is associated with positive lymph node status in patients with recurrence of colorectal cancer?indications for anti-TNF-? agents in cancer treatment

This paper focuses on the analysis of average Gaussian error probabilities in certain fading channels, i.e. we are interested in E[Q((p {\\gamma})^(1/2))] where Q(.) is the Gaussian Q-function, p is a positive real number and {\\gamma} is a nonnegative random variable. We present a unified analysis of the average Gaussian error probability, derive a compact expression in terms of the Lauricella FD^(n) function that is applicable to a broad class of fading channels, and discuss the relation of this expression and expressions of this type recently appeared in literature. As an intermediate step in our derivations, we also obtain a compact expression for the outage probability of the same class of fading channels. Finally, we show how this unified analysis allows us to obtain novel performance analytical results.

2011-01-01

259

British Library Electronic Table of Contents (United Kingdom)

Introduction The progressive growth of malignancies is accompanied by a decline in the immune response through mechanisms which are poorly understood. Apoptosis and induction of inflammation by tumor released cytokines as tumor escape mechanisms have been proposed to play an important role in colorectal carcinogenesis. Methods Expression of Tumor necrosis factor-alpha (TNF-?) was analyzed in colorectal cancer specimen and the cancer cell line HT-29 by immunohistochemistry and RT-PCR. TNF-? expression on protein and mRNA level were correlated with clinical characteristics and impact on survival. TNFR-1 was co-labelled with TNF-? and CD8+ cytotoxic T cells in immunofluorescence double staining experiments. Results: 94% (n?=?98/104) of the patients with CRC expressed TNF-?. High TNF-? express...

2011-01-01

260

The role of fatty acid desaturases in epidermal metabolism

The expression pattern of scygonadin during the ontogenesis of Scylla paramamosain predicting its potential role in reproductive immunity

The lipid composition of skin is important to a variety of functions served by this organ. Therefore, skin expresses multiple enzymes that synthesize and metabolize lipids. An important class of lipid...Full Text Available

2011-04-01

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261

British Library Electronic Table of Contents (United Kingdom)

The antimicrobial peptide scygonadin (Scy) was first isolated from the gonad of Scylla serrata and its gene is predominantly expressed in the ejaculatory duct of adult males. Thus, its function was predicted to be associated with reproductive immunity, but this is still unclear and needs further investigation. In our study, the expression pattern of Scy at different developmental stages of both male and female S. paramamosain was investigated, so that the potential function of this peptide could be examined. Using real-time quantitative PCR, Scy mRNA transcripts were demonstrated obviously in the vulnerable embryos and larvae-zoea I but very weakly detected in the larvae-zoea III, megalops and juveniles. The gene expression pattern showed a decreasing trend during the early developmental s...

2011-01-01

262

The CMV early enhancer/chicken beta actin (CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors.

Significance of Aurora B overexpression in hepatocellular carcinoma. Aurora B Overexpression in HCC

Udgivelsesdato: 2008-null

2008-01-01

263

Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in crohn's disease

BackgroundTo investigate the significance of Aurora B expression in hepatocellular carcinoma (HCC).MethodsThe Aurora B and Aurora...Full Text Available

264

British Library Electronic Table of Contents (United Kingdom)

AbstractBackground: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD). Methods: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25). Results: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) with...

2011-01-01

265

Role of Fgf receptor 2c in adipocyte hypertrophy in mesenteric white adipose tissue

British Library Electronic Table of Contents (United Kingdom)

Fgf receptor 2c (Fgfr2c) was expressed in mature adipocytes of mouse white adipose tissue (WAT). To examine the role of Fgfr2c in mature adipocytes, we generated adipocyte-specific Fgfr2 knockout (Fgfr2 CKO) mice. The hypertrophy impairment of adipocytes in the mesenteric WAT but not in the subcutaneous WAT and decreased plasma free fatty acid (FFA) levels were observed in Fgfr2 CKO mice. Although the expression of genes involved in adipocyte differentiation and lipid metabolism in the mesenteric WAT was essentially unchanged, the expression of uncoupling protein 2 potentially involved in energy dissipation was significantly increased. Among potential Fgf ligands for Fgfr2c, Fgf9 was preferentially expressed in the mesenteric WAT. The present findings indicate that Fgfr2c potentially activ...

2008-01-01

266

Ras activation of genes: Mob-1 as a model.

Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

The ras oncogenes function by indirectly controlling expression of a subset of yet-undefined genes that are crucial for cell growth and differentiation. In a differential display strategy, numerous...Full Text Available

1994-12-20

267

Protein expression profile from the Mesocestoides corti (Platyhelminthes: Cestoda) larval stage

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268

Possible Implications of Placental Expressions for Heat Shock Protein 70 and Lectin-like Oxidized Low Density Lipoprotein Receptor-1 on Postnatal Blood Pressure in Their Newborns

English 2007 [1 p.] Brazil Laschuk, A. Silveira, PA da Bizarro, CV Carvalho,

2007-05-21

269

Physiologic changes during growth and development.

Full Text Available

2007-09-01

270

Overexpression of MAD2 predicts clinical outcome in primary lung cancer patients

To express growth-related changes in physiologic or other functions in forms usable for kinetic modeling, we are interested in identifying regular relationships that take the form of simple mathematical...Full Text Available

1994-12-01

271

British Library Electronic Table of Contents (United Kingdom)

High-level expression of mitotic arrest defective protein 2 (MAD2), a central component of the spindle assembly checkpoint, has been observed in a variety of human malignancies. Aim of the present study was to observe the expression of MAD2 in human non-small-cell lung cancer (NSCLC) and explore its clinicopathologic significance and evaluate MAD2 expression as a prognostic marker. MAD2 transcript was found to be overexpressed in the great majority of lung cancers by semi-quantitative RT-PCR. A total of 358 NSCLCs were analyzed immunohistochemically on tissue microarrays. High-level MAD2 expression was observed in 26.3% (94 of 358 cases), and correlated with male sex (P=0.0002), tumor progression (pT status) (P=0.0009), visceral or parietal pleural invasion (P=0.0151), non-adenocarcinoma, ...

2011-01-01

272

Ovarian Gene Expression is Stable after Exposure to Trichloroethylene

Norepinephrine represses the expression of toxA and the siderophore genes in Pseudomonas aeruginosa

Exposure of female rats to trichloroethylene (TCE), an environmental toxicant commonly found in ground and surface waters throughout the United States, reduces the fertilizability of oocytes...Full Text Available

2008-02-28

273

Molecular cloning and analysis of lymphokines. Volume 13

Among the different extracellular virulence factors produced by Pseudomonas aeruginosa are exotoxin A (ETA) and the pyoverdine and pyochelin siderophores. Production of ETA...Full Text Available

2009-10-01

274

Microarray-based gene expression profiles of silkworm brains

These proceedings collect papers on the subject of lymphokines. Topics include: DNA-cloning of mouse and human lymphokine genes, inteferons, interleukins, gene expression, tumor necrosis factors, and recombinant DNA.

1987-01-01

275

Induced Expression of Doublesex-and mab-3-related Transcription Factor-1 (DMRT-1) mRNA by Testosterone in the Olive Flounder, Paralichthys olivaceus ovary

BackgroundMolecular genetic studies of Bombyx mori have led to profound advances in our understanding of the regulation of development. Bombyx mori brain,...Full Text Available

276

IGF 2 expression in 3T3 adipocytes in response to serum from hypophysectomized or diabetic swine

Full Text Available

2007-08-01

277

Hypoxia decreases sclerostin expression and increases Wnt signaling in osteoblasts

Expression of IGF-2 and changes in its expression in response to systemic endocrine alterations have not been demonstrated for adipocytes. Adipocytes were induced to develop within cultures of 3T3-L1 cells using a medium containing 0.5mM isobutylmethylxanthine, 1uM insulin and 100ng hydrocortisone/ml for 48 hours of exposure. Cultures containing developing adipocytes were incubated with 10% pig serum and 1 uM insulin for several days. The resultant adipocyte cultures were then treated with either 10% pig serum, diabetic pig serum or hypophysectomized pig serum in DMEM for 48 hours. Adipocytes within the cultures were separated from undifferentiated cells using percoll density gradient centrifugation. Total RNA was isolated from adipocytes and dot blotted. Blots were probed with a {sup 32}P-cDNA probe for rat IGF-2. IGF-2 was expressed by the adipocytes and the pattern of expression showed specific ...

1990-02-26

278

British Library Electronic Table of Contents (United Kingdom)

Mutations in sclerostin function or expression cause sclerosing bone dysplasias, involving decreased antagonism of Wnt/Lrp5 signaling. Conversely, deletion of the VHL tumor suppressor in osteoblasts, which stabilize HIF-a isoforms and thereby enables HIF-a/b-driven gene transcription, increases bone mineral content and cross-sectional area compared to wild-type controls. We examined the influence of cellular hypoxia (1% oxygen) upon sclerostin expression and canonical Wnt signaling. Osteoblasts and osteocytes cultured under hypoxia revealed decreased sclerostin transcript and protein, and increased expression and nuclear localization of activated b-catenin. Similarly, both hypoxia and the hypoxia mimetic DFO increased b-catenin gene reporter activity. Hypoxia and its mimetics increased exp...

2010-01-01

279

Hypothyroidism Enhances Tumor Invasiveness and Metastasis Development

Gpnmb is a Melanoblast-Expressed, MITF-Dependent Gene

BackgroundWhereas there is increasing evidence that loss of expression and/or function of the thyroid hormone receptors (TRs) could result in a selective advantage for tumor development,...Full Text Available

280

Global Transcriptional Responses of Fission Yeast to Environmental Stress

SUMMARYExpression profile analysis clusters Gpnmb with known pigment genes, Tyrp1, Dct, and Si. During development,...Full Text Available

2009-02-01

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281

Genomic imprinting and the social brain

We explored transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses. DNA microarrays were used to characterize changes in expression...Full Text Available

2003-01-01

282

Expression of an ampG like gene in Herbaspirillum seropedicae strain SmR1 is regulated by naringenin and its product appears to be involved in the determination of LPS structure

Genomic imprinting refers to the parent-of-origin-specific epigenetic marking of a number of genes. This epigenetic mark leads to a bias in expression between maternally and paternally inherited imprinted...Full Text Available

2006-12-29

283

Expression and regulation of the NALP3 inflammasome complex in periodontal diseases

No abstract prepared.

2009-07-01

284

Endogenous expression of Hras(G12V) induces developmental defects and neoplasms with copy number imbalances of the oncogene.

Periodontitis is an infectious process characterized by inflammation affecting the supporting structures of the teeth. Porphyromonas gingivalis is a major oral bacterial species implicated...Full Text Available

2009-09-01

285

Coreference and Lexical Repetition: Mechanisms of Discourse Integration

We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms of tumor initiation. They had high perinatal mortality, abnormal cranial dimensions, defective dental ameloblasts, and nasal septal deviation, consistent with some of the features of human Costello syndrome. These mice developed papillomas and angiosarcomas, which were associated with Hras(G12V) allelic imbalance and augmented Hras signaling. Endogenous expression of Hras(G12V) was also associated with a higher mutation rate in vivo. Tumor initiation by Hras(G12V) likely requires augmentation of signal output, which in papillomas and angiosarcomas is achieved via increased Hras-gene copy number, which may be favored by a higher mutation frequency in cells expressing the oncoprotein. PMID:19416908

2009-04-29

286

Conditional Circadian Regulation of PHYTOCHROME A Gene Expression

The use of repeated expressions to establish coreference allows an investigation of the relationship between basic processes of word recognition and higher-level...Full Text Available

2007-06-01

287

Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides...Full Text Available

2001-12-01

288

British Library Electronic Table of Contents (United Kingdom)

To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-...

2011-01-01

289

Characterization of lymphocyte receptors for glycosaminoglycans.

Bioengineered bugs expressing oligosaccharide receptor mimics

This paper describes attempts to isolate and characterize glycosaminoglycan (GAG)-binding molecules on the surface of lymphocytes and lymphoma cell lines and relate their expression to splenic and lymph...Full Text Available

1991-02-01

290

Association and expression study of synapsin III and schizophrenia

Many microbial pathogens recognize oligosaccharides displayed on the surface of host cells as receptors for toxins and adhesins. These ligand-receptor interactions are critical for disease pathogenesis,...Full Text Available

2010-05-01

291

An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter...Full Text Available

2009-11-20

292

British Library Electronic Table of Contents (United Kingdom)

Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (Epinephelus coioides) fry. However, the incubation period was determined to be over 2weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found. Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant V. anguilla...

2011-01-01

293

What are $E_{\\infty}$ ring spaces good for?

Ultrafast resonance energy transfer in bio-molecular systems

Infinite loop space theory, both additive and multiplicative, arose largely from two basic motivations. One was to solve calculational questions in geometric topology. The other was to better understand algebraic K-theory. The Adams conjecture is intrinsic to the first motivation, and Quillen's proof of that led directly to his original, calculationally accessible, definition of algebraic K-theory. In turn, the infinite loop understanding of algebraic K-theory feeds back into the calculational questions in geometric topology. For example, use of infinite loop space theory leads to a method for determining the characteristic classes for topological bundles (at odd primes) in terms of the cohomology of finite groups. We explain just a little about how all that works, focusing on the central role played by E infinity ring spaces.

2009-01-01

294

British Library Electronic Table of Contents (United Kingdom)

In this article, we present our consistent efforts to explore the dynamical pathways of the migration of electronic radiation by using ultrafast (picosecond/femtosecond time scales) F?rster resonance energy transfer (FRET) technique. The ultrafast non-radiative energy migration from an intrinsic donor fluorophore (Tryptophan, Trp214) present in domain IIA of a transporter protein human serum albumin (HSA) to various non-covalently/covalently attached organic/inorganic chromophores including photoporphyrin IX (PPIX), polyoxovanadate [V15As6O42(H2O)]-6 clusters (denoted as V15) and CdS quantum dots (QDs) has been explored. We have also used other covalently/non-covalently attached extrinsic fluorogenic donors (NPA, ANS) in order to exploit the dynamics of resonance energy migration of an enz...

2010-01-01

295

UK Achievements in the application of power fluidic technology for nuclear processing plants

Transport properties of single-crystalline n-type semiconducting PbTe nanowires

Power Fluidic systems for the control of liquids and gaseous flows have been adopted for use in radioactive processing plants in the UK. These devices are intrinsically reliable with no mechanical moving parts because they are able to make use of the hydrodynamics of the fluids being controlled. This reliability feature leads to a zero cell maintenance concept and the elimination of mechanical drive/control systems in cell. The first phase of the development work led to their use in the Fast Reactor Reprocessing Plant at Dounreay and the Highly Active Liquor Storage facility at Sellafield. The success of these early developments has led to an extensive development programme for an extended range of applications in the Thermal Oxide Reprocessing Plant and its associated waste treatment facilities at Sellafield. The technology has now been fully demonstrated and adopted for these plants with considerable benefit over a wide range of applications.

296

Tracheal cross-sectional area in children: CT determination

Single-crystalline PbTe nanowires were synthesized using the chemical vapor transport method. They consisted of rock-salt structure PbTe nanocrystals uniformly grown in the [100] direction. We fabricated field-effect transistors using a single PbTe nanowire, providing evidence for its intrinsic n-type semiconductor characteristics. The values of the carrier mobility and concentration were estimated to be 0.83 cm"2 V"-"1 s"-"1 and 8.8 x 10"1"7 cm"-"3, respectively. The Seebeck coefficients (-72 ?V K"-"1) of individual nanowires were measured to show their n-type carrier-dominated thermoelectric transport properties.

2009-10-14

297

Three-dimensional modeling of heat transport in deep hydrothermal reservoirs

A computer method that calculates tracheal cross-sectional area by compensating for partial volume averaging was developed and validated in a study with phantoms. The program was then used to determine the tracheal cross-sectional area of 30 normal children who ranged in age from four months to 18 years. CT-derived cross sections were correlated with age, height, weight, and body-surface area, and they were compared with findings of published clinical and post-mortem studies. CT cross-sectional areas ranged from 20 to 275 mm/sup 2/, varied by as much as 22% at the three different tracheal levels studied, and appeared to correlate most closely with body height. CT-derived tracheal cross-sectional areas are quite similar to those in published reports of postmortem and clinical studies. Measurements of tracheal cross section by CT may prove useful in quantitating tracheal compromise by intrinsic or extrinsic causes.

1983-10-01

298

British Library Electronic Table of Contents (United Kingdom)

The need for alternative energy increases steadily???especially due to the climate change and the limited availability of fossil fuels. Geothermal power uses the intrinsic heat which is stored in the accessible part of the Earth???s crust. Its importance among the renewable energy resources originates from the almost unlimited energy supply of the Earth and its independence from external influences such as seasonal or even daily climatic variability. Nevertheless, there are risks which have to be assessed. From a mathematical point of view there are four building blocks of the characterization of deep geothermal systems: seismic exploration, gravimetry, modeling transport processes, and modeling the stress field. In particular, local depletion poses a significant risk during the industrial...

2011-01-01

299

The temporal evolution of coronal loops observed by GOES-SXI

The microscopic dynamics of condensed parahydrogen

We study the temporal evolution of coronal loops using data from the Solar X-ray Imager (SXI) on board of GOES-12. This instrument allows us to follow in detail the full lifetime of coronal loops. The observed light curves suggest three somewhat distinct evolutionary phases: rise, main, and decay. The durations and characteristic timescales of these phases are much longer than a cooling time and indicate that the loop-averaged heating rate increases slowly, reaches a maintenance level, and then decreases slowly. This suggests that a single heating mechanism operates for the entire lifetime of the loop. For monolithic loops, the loop-averaged heating rate is the intrinsic energy release rate of the heating mechanism. For loops that are bundles of impulsively heated strands, it is an indication of the frequency of occurrence of individual heating events, or nanoflares. We show that the timescale of the loop-averaged heating rate is proportional to the timescale of ...

2006-01-01

300

The gamma radiation in improving the physical properties of wood-polymer composites

We have measured the almost-pure incoherent scattering function of liquid and solid parahydrogen, using inelastic neutron scattering. The experiments were carried out on TOSCA, a time-of-flight, inverse-geometry, crystal-analyzer spectrometer, operating on the pulsed neutron source at ISIS (UK). The experiments have been planned taking advantage of the intrinsic incoherence introduced in the scattering process by the rotational transitions. The measured double-differential cross section gives a direct experimental access to the microscopic dynamics of condensed hydrogen. From the high-energy region of the spectrum, where the impulse approximation for the center of mass motion applies, we have been able to extract the translational mean kinetic energy. In the low-energy region, using the Gaussian approximation, we compare the experimental data in the liquid phase with the results of a model for the velocity autocorrelation function. The results are encouraging, but ...

2002-07-01

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301

The Bipolar Field-Effect Transistor: VIII. Longitudinal Gradient of Longitudinal Electric Field (Two-MOS-Gates on Pure-Base)

In the Peruvian natural forest there are several kind of woods, some of those are not of commercial interest, because have not resistance at termites and wet, are too soft, or can not be well finished, in the others. The present work is devoted to improvement of the physical properties of wood, such as hardness, traction, wet resistance, etc., by means of the generation of a composite wood-polymers cured by using gamma irradiation. The main objective is to establish the main parameters for the polymerization process (economic technical aspect) that decrease the amount of water absorption, in consequence the volumetric variation of Catahua, Mohena and Capirona woods. Gamma irradiation also provides good protection against the biological agents. In consequence, these woods increase its intrinsic and economic values. (author)

2006-07-01

302

Superconductivity in the ternary rare-earth (Y, La, and Lu) compounds RPd_2Si_2 and RRh_2Si_2

This paper evaluates the electric current terms from the longitudinal gradient of the longitudinal electric field in Bipolar Field-Effect-Transistors (BiFETs) with a pure base and two MOS gates operating in the unipolar (electron) current mode. These nMOS-BiFETs, known as nMOS-FinFETs, usually have electrically short channels compared with their intrinsic Debye length of about 25 #mu#m at room temperatures. These longitudinal electric current terms are important short-channel current components, which have been neglected in the computation of the long-channel electrical characteristics. This paper shows that the long-channel electrical characteristics are substantially modified by the longitudinal electrical current terms when the physical channel length is less than 100 nm.

2010-07-01

303

Sedimentation of the Enan suite of the lower to middle Jurassic and coal reserves of the southeastern margin of the Ordos basin (China)

We have investigated the superconducting and metallurgical properties of the ternary compounds RPd_2Si_2 and RRh_2Si_2 with R = Y, La, and Lu. All RPd_2Si_2 compounds and LaRh_2Si_2 were found to be type-I superconductors below 1 K. A detailed metallurgical analysis shows that segregation of second phases can easily mask the intrinsic (stoichiometric ratio 1:2:2) intermetallic-compound properties. Two sample-preparation techniques, viz., single crystals and off-stoichiometry, were utilized to establish where bulk superconductivity occurs. The type-I behavior of these compounds is explained with an analogous model as is used for the heavy-fermion superconductors CeCu_2Si_2 and URu_2Si_2.

304

SZ polarisation as a probe of the intracluster medium

The studied coal bearing series has a parallel origin. The following facial complexes are isolated in its composition: lakes free of vegetation without coal interlayers; overgrown lakes of their margins which are poor in coals; swampy lakes, whose peat beds formed coal layers of shallow or moderate depth; channel sediments without coal layers and swamps from between channel spaces which created thick coal strata of high quality for which, however, a strong changeability in thickness is intrinsic. The coal strata are normally split towards the central parts of the lake basins and river channels. The coal presence of the suite is reduced along the section which is associated with the replacement of the lake swamp conditions of sedimentation by lake river conditions. A paleotectonic analysis established that the distribution of the zones richest in coal was controlled by consedimentational structural elements.

1983-01-01

305

Practical applications and feasibility of efflux pump inhibitors in the clinic-A vision for applied use

We present high-resolution hydrodynamical simulations of the degree and direction of polarisation imprinted on the CMB by the Sunyaev-Zel'dovich effect in the the line of sight to massive galaxy clusters. We focus on two contributions which contribute most of the induced CMB polarisation in addition to the intrinsic CMB quadrupole: the radiation quadrupole seen by electrons due to their own velocity in the plane normal to the line of sight, and the radiation quadrupole due to the thermal Sunyaev-Zel'dovich effect, which is generated by a previous scattering elsewhere in the cores of the local and nearby clusters. We show that inside the virial radius of a massive cluster, this latter effect, although being second order in the optical depth, can reach the level of the former effect. These two effects can, respectively, constrain the projected tangential velocity and inner density profile of the gas, if they can be separated with multi-frequency observations. As the ...

2004-01-01

306

British Library Electronic Table of Contents (United Kingdom)

The world of antibiotic drug discovery and development is driven by the necessity to overcome antibiotic resistance in common Gram-positive and Gram-negative pathogens. However, the lack of Gram-negative activity among both recently approved antibiotics and compounds in the developmental pipeline is a general trend despite the fact that the plethora of covered drug targets are well-conserved across the bacterial kingdom. Such intrinsic resistance in Gram-negative bacteria is largely attributed to the activity of multidrug resistance (MDR) efflux pumps. Moreover, these pumps also play a significant role in acquired clinical resistance. Together, these considerations make efflux pumps attractive targets for inhibition in that the resultant efflux pump inhibitor (EPI)/antibiotic combination d...

2006-01-01

307

Point defects in dilute nitride III-N-As and III-N-P

Phenomenological dynamics of loop quantum cosmology in Kantowski-Sachs spacetime

We provide a brief review of our recent results from optically detected magnetic resonance studies of grown-in non-radiative defects in two most important dilute nitride systems-Ga(In)NAs grown on GaAs substrates and Ga(Al,In)NP grown on Si and GaP substrates. These results have led to the identification of defect complexes in the alloys, involving intrinsic defects such as As_G_a antisites and Ga_i self-interstitials. They have also shed light on formation mechanisms of the defects and on their role in non-radiative carrier recombination that is harmful to the performance of potential optoelectronic and photonic devices based on these dilute nitrides.

2006-04-01

308

Petroleum storage tank cleaning using commercial microbial culture products

The full theory and the semiclassical description of loop quantum cosmology (LQC) have been studied in the Friedmann-Robertson-Walker and Bianchi I models. As an extension to include both anisotropy and intrinsic curvature, this paper investigates the cosmological model of Kantowski-Sachs spacetime with a free massless scalar field at the level of phenomenological dynamics with the LQC discreteness corrections. The LQC corrections are implemented in two different improved quantization schemes. In both schemes, the big bang and big crunch singularities of the classical solution are resolved and replaced by the big bounces when the area or volume scale factor approaches the critical values in the Planck regime measured by the reference of the scalar field momentum. Symmetries of scaling are also noted and suggest that the fundamental spatial scale (area gap) may give rise to a temporal scale. The bouncing scenarios are in an analogous fashion of the Bianchi I model, ...

2008-01-01

309

Paroxysmal nocturnal hemoglobinuria: a case report of MR, CT findings

The removal of paraffinic bottom accumulations from refinery storage tanks represents an increasingly costly area of petroleum storage management. Microorganisms can be used to reduce paraffinic bottoms by increasing the solubility of bottom material and by increasing the wax-carrying capacity of carrier oil used in the cleaning process. The economic savings of such treatments are considerable. The process is also intrinsically safer than alternative methods, as it reduces and even eliminates the need for personnel to enter the tank during the cleaning process. Both laboratory and field sample analyses can be used to document changes in tank material during the treatment process. These changes include increases in volatile content and changes in wax distribution. Several case histories illustrating these physical and chemical changes are presented along with the economics of treatment.

1995-12-31

310

Overview of the recent activities of the RD50 collaboration on radiation hardening of semiconductor detectors for the sLHC

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare, acquired disease involving multiple hematopoietic cell lines. Characteristics of PNH are intrinsic hemolytic anemia, iron deficiency anemia and venous thrombosis. We report a case of PNH with characterostoc MR and CT findings. The signal intensity of renal cortex was lower than that of medulla on both T1-and T2-weighted MR imaging. On T2 weighted MR images, the liver showed very low signal intensity but the signal intensity of the spleen was normal. On precontrast CT the attenuation of renal cortex was higher than that of renal medulla and the attenuation of liver was higher than that of the spleen. These findings of MR imaging and CT were the result from the deposition of hemosiderin in the cells of proximal convoluted tubules and transfusional hemosiderosis of liver.

1995-10-15

311

Overview of the recent activities of the RD50 collaboration on radiation hardening of semiconductor detectors for the sLHC

The RD50 collaboration has been exploring the development of radiation hard semiconductor devices for very high-luminosity colliders since 2002. The target fluence to qualify detectors set by the anticipated dose for the innermost tracking layers of the future upgrade of the CERN large hadron collider (LHC) is 1016 1 MeV neutron equivalent (neq) cm-2. This is about an order of magnitude higher than the maximum dose for the most exposed silicon detectors in the current machine. RD50 investigates the radiation hardening of silicon sensors from many angles: improvement of the intrinsic tolerance of the substrate material, optimisation of the readout geometry and study of novel design of detectors. A review of some of the recent activities within RD50 is here presented.

2009-01-01

312

British Library Electronic Table of Contents (United Kingdom)

The RD50 collaboration has been exploring the development of radiation hard semiconductor devices for very high-luminosity colliders since 2002. The target fluence to qualify detectors set by the anticipated dose for the innermost tracking layers of the future upgrade of the CERN large hadron collider (LHC) is 1016 1MeV neutron equivalent (neq) cm-2. This is about an order of magnitude higher than the maximum dose for the most exposed silicon detectors in the current machine. RD50 investigates the radiation hardening of silicon sensors from many angles: improvement of the intrinsic tolerance of the substrate material, optimisation of the readout geometry and study of novel design of detectors. A review of some of the recent activities within RD50 is here presented.

2009-01-01

313

Origin of complex quantum amplitudes and Feynman's rules

Optimal dynamic detection of explosives

Complex numbers are an intrinsic part of the mathematical formalism of quantum theory and are perhaps its most characteristic feature. In this article, we show that the complex nature of the quantum formalism can be derived directly from the assumption that a pair of real numbers is associated with each sequence of measurement outcomes, with the probability of this sequence being a real-valued function of this number pair. By making use of elementary symmetry conditions, and without assuming that these real number pairs have any other algebraic structure, we show that these pairs must be manipulated according to the rules of complex arithmetic. We demonstrate that these complex numbers combine according to Feynman's sum and product rules, with the modulus-squared yielding the probability of a sequence of outcomes.

2010-02-01

314

ON THE PROGENITOR AND EARLY EVOLUTION OF THE TYPE II SUPERNOVA 2009kr

The detection of explosives is a notoriously difficult problem, especially at stand-off distances, due to their (generally) low vapor pressure, environmental and matrix interferences, and packaging. We are exploring optimal dynamic detection to exploit the best capabilities of recent advances in laser technology and recent discoveries in optimal shaping of laser pulses for control of molecular processes to significantly enhance the standoff detection of explosives. The core of the ODD-Ex technique is the introduction of optimally shaped laser pulses to simultaneously enhance sensitivity of explosives signatures while reducing the influence of noise and the signals from background interferents in the field (increase selectivity). These goals are being addressed by operating in an optimal nonlinear fashion, typically with a single shaped laser pulse inherently containing within it coherently locked control and probe sub-pulses. With sufficient bandwidth, the technique is capable of ...

2009-01-01

315

Neutral-meson oscillations with torsion

We identify a source coincident with SN 2009kr in Hubble Space Telescope pre-explosion images. The object appears to be a single point source with an intrinsic color V - I = 1.1 #+-# 0.25 and M_V = -7.6 #+-# 0.6. If this is a single star, it would be a yellow supergiant of log L/L _s_u_n #approx# 5.1 and a mass of 15"+"5 _-_4 M _s_u_n. The spatial resolution does not allow us yet to definitively determine if the progenitor object is a single star, a binary system, or a compact cluster. We show that the early light curve is similar to a Type IIL SN, but the prominent H#alpha# P-Cygni profiles and the signature of the end of a recombination phase are reminiscent of a Type IIP. The evolution of the expanding ejecta will play an important role in understanding the progenitor object.

2010-05-10

316

Modeling of phosphorus diffusion in Ge accounting for a cubic dependence of the diffusivity with the electron concentration

We propose a simple mechanism that may explain the observed particle-antiparticle asymmetry in the Universe. In the Einstein-Cartan-Sciama-Kibble theory of gravity, the intrinsic spin of matter generates spacetime torsion. Classical Dirac fields in the presence of torsion obey the nonlinear Hehl-Datta equation which is asymmetric under a charge-conjugation transformation. Accordingly, at extremely high densities that existed in the very early Universe, fermions have higher effective masses than antifermions. As a result, a meson composed of a light quark and a heavy antiquark has a lower effective mass than its antiparticle. Neutral-meson oscillations in thermal equilibrium therefore favor the production of light quarks and heavy antiquarks, which may be related to baryogenesis.

2011-01-01

317

Microstructure characterization and tensile properties of b phase containing TiAl pancake

Up to now, P diffusion in Ge is modeled with an effective diffusivity involving at most a quadratic dependence with the free electron concentration (n). However, recent theoretical studies suggest the existence of a triply negatively charged state for the free vacancy in germanium and experimental data indicate that the E center (PV pair) in Ge has a double acceptor state. These two facts would be consistent with a diffusivity model involving a cubic dependence with n. In this paper the validity of this approach is checked for both pure thermal diffusion (intrinsic and extrinsic) and implanted phosphorus, using either our own experiments or other data available from the literature. Although some discrepancies still exist in some cases for the redistribution of implanted P, it is shown that the introduction of this cubic dependence significantly improves the overall agreement as compared with the usual model.

2010-02-26

318

British Library Electronic Table of Contents (United Kingdom)

The microstructure and tensile properties of Ti-44Al-6V-3Nb-0.3Y (at.%) alloy after canned forging were investigated. SEM results showed that the TiAl pancake exhibits inhomogeneous microstructure, which can be ascribed to the temperature drop and friction between billet and outer pack during forging, as well as the intrinsic anisotropy of lamellar colony. By means of TEM observation and EBSD analysis, the microstructure in the dominant area of the pancake was further characterized. This deformation area consists of 87.7% content of g grains plus some refined lamellar colonies and the rest of B2 grains. The grain size ranges between 1mm and 8.5mm. High-angle boundaries dominate the deformation microstructure, several substructures and twins are observed as well. Additionally, current forge...

2011-01-01

319

Magnetic refrigeration: the promise and the problems

Local lattice structure, crystal field and energy level patterns in CsCdBr{sub 3}:Tm{sup 3+} crystals

Magnetic refrigeration uses the temperature- and field-dependence of the entropy of some magnetic materials to accomplish cooling. Because of the intrinsically high efficiency of the magnetization and demagnetization process and because of the potential for excellent heat transfer between solids and fluids, magnetic refrigerators promise to have higher efficiency than existing gas-cycle refrigerators. Many ground-based and space-based applications could benefit significantly from the cost savings implied by higher efficiency. Other attributes of these devices are high reliability and low volume and mass per unit cooling power. The development of these refrigerators is underway at several places around the world, including the Los Alamos National Laboratory. The progress to date has been encouraging but some problems have been clearly identified. The arguments for high efficiency and the problems that will need to be solved to achieve this goal are discussed.

1984-01-01

320

Junction conditions in General Relativity with spin sources

In CsCdBr{sub 3}, Tm{sup 3+} substitutes for Cd{sup 2+}. It predominately forms symmetric dimer centers and single-ion centers, both of trigonal symmetry. The energy level schemes of both centers were determined by EPR and site-selective laser spectroscopy. To describe the spectra term dependent crystal-field parameters were deduced on the basis of a microscopic model taking into account the local lattice deformation induced by the impurity centers and the quasi-resonant virtual scattering of intrinsic lattice excitations by the Tm{sup 3+} ions. (orig.) 22 refs.

1998-07-24

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321

Investigation of electronic traps in disordered organic semiconductors via thermally stimulated current measurements

The junction conditions for General Relativity in the presence of domain walls with intrinsic spin are derived in three and higher dimensions. A stress tensor and a spin current can be defined just by requiring the existence of a well defined volume element instead of an induced metric, so as to allow for generic torsion sources. In general, when the torsion is localized on the domain wall, it is necessary to relax the continuity of the tangential components of the vielbein. In fact it is found that the spin current is proportional to the jump in the vielbein and the stress-energy tensor is proportional to the jump in the spin connection. The consistency of the junction conditions implies a constraint between the direction of flow of energy and the orientation of the spin. As an application, we derive the circularly symmetric solutions for both the rotating string with tension and the spinning dust string in three dimensions. The rotating string with tension ...

2006-01-01

322

Interaction of insulin with a triblock copolymer of PEG-(fumaric-sebacic acids)-PEG: Thermodynamic and spectroscopic studies

Charge transport in disordered organic semiconductors is generally described as thermally activated hopping in a gaussian distribution of localized states. The presence of charge traps is critical to the performance of organic electronic devices, since trapped charge carriers do no longer contribute to the current flow. The trap distribution in the polymer poly(3-hexylthiophene) (P3HT) is investigated by applying the fractional thermally stimulated current technique. Thereby, a low temperatur double-peak distribution has been revealed. One of the peaks is believed to belong to the tail of the intrinsic density of states, whereas the other trap is strongly affected by exposure to oxygen. We discuss the influence of oxygen exposure time on the trap distribution.

2008-07-01

323

British Library Electronic Table of Contents (United Kingdom)

A comparative study on the interaction of (PEG-co-P(FA/SC)-co-PEG) triblock copolymer with bovine and human insulins was carried out using isothermal titration calorimetry (ITC), circular dichroism (CD), and fluorescence spectroscopy. ITC data show that the copolymer has a low affinity for both proteins, with an association constant of about 7?9?103?M ?1. Results also show that binding is enthalpically driven, and disfavored by conformational entropy. CD spectroscopy studies reveal a small increase in the helical content and a decrease in ?-structure as well as random coil in both proteins. Acrylamide quenching experiments display reduced accessibility of tyrosines, while intrinsic fluorescence spectra show lower tyrosine emission. Furthermore, thermal unfolding experiments, studied by far...

2007-01-01

324

Influence of mass transfer on thermogravimetric analysis of combustion and gasification reactivity of coke

British Library Electronic Table of Contents (United Kingdom)

Non-isothermal TG/DTG measurements are widely used to determine kinetic parameters of oxidation and gasification of carbons, e.g. by multi-heating rate methods. Thereby it is important to avoid spurious effects due to diffusion limitations. In this work, TG/DTG experiments with activated carbon were conducted under isothermal and non-isothermal conditions. The agreement of the experimental results with results of simulations based on the intrinsic kinetics, pore diffusion and external diffusion in/to the sample (crucible) is very good. The simulations show that mass transfer has an unwanted influence on TG measurements, if the concentration of the gaseous reactant (O2 and CO2) is too low and/or the heating rate is too high. Based on literature data, the simulation was extended to blast fur...

2010-01-01

325

In situ strain measurements during the formation of palladium silicide films

Hydrophobic labelling of membrane-embedded proteins with lipophilic reagents

The evolution of strain in the Pd-Si system during the growth of Pd{sub 2}Si thin films on Si (100) substrate has been followed in situ using a double optical beam technique. As was observed for the Pt-Si system, the reaction to form Pd{sub 2}Si yields a compressive intrinsic surface film stress as well as for the silicon-rich suicides as proposed by Angilello et al. [Thin Film Interfaces and Interactions, edited by J. Baglin and J. Poate (The Electrochemical Society, Pennington, NJ, 1980)]. A transmission electron microscopy analysis has revealed grain growth during the formation of Pd{sub 2}Si which cannot account for the compressive film stresses. The formation of silicide at the interfaces rather than the overall change in volume agrees with the sign of the stresses formed. 29 refs., 4 figs., 3 tabs.

1993-03-01

326

Human brain development in infants with PET and FDG

Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymhocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of ["1"2"5I]iodonaphtylazide ["1"2"5I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymhpocytes with ["1"2"5I]INA and commercially available reagent 3-(trifluoromethyl)-3-(trifluoromethyl)-3-(3'-["1"2"5Iliodophenyl)diazirine (["1"2"5I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. (author). 28 refs.; 4 figs.

327

High-temperature hysteretic electronic effects of (Al{sub x}Ga{sub 1{minus}x}){sub 0.5}In{sub 0.5}P (x > 0.65)

The authors used studies of local cerebral metabolic rate for glucose (LCMRGlc) to examine development of cerebral organization in 5 days to 1 year old children. A group (n=8) of infants with diverse pediatric disorders allowed investigation of developmental changes in LCMRGlc, while also providing relevant clinical management information. Patients consisted of questionable and definite neonatal seizures, cerebral embolism from cardiac sources, and otherwise normal infants with facial nevi with consideration of Sturge-Weber. Gradual increase in cortical LCMRGlc coincides with suppression of intrinsic subcortical reflexes present in all newborns. Two retarded children (2 years old) showed LCMRGlc developmental patterns of a few days old, which corresponded to their functional and mental status. These studies illustrate great potential of PET to study normal and altered states of human brain development.

1985-06-02

328

High-temperature hysteretic electronic effects of (Al{sub x}Ga{sub 1{minus}x}){sub 0.5}In{sub 0.5}P (x > 0.65)

The authors have studied (Al{sub x}Ga{sub 1{minus}x}){sub 0.5}In{sub 0.5}P doped with tellurium using deep level transient spectroscopy and associated electrical measurements. Several defect states are observed in the upper half of the band gap, that are believed to be intrinsic to the alloy system as well as related to the tellurium donors. Defects observed at measurement temperatures above 390 K exhibit a hysteretic behavior. The observed spectra depend on the biasing conditions applied to the Schottky diode during cooling. The hysteretic behavior suggests the existence of different defect configurations, which can be accessed under conditions of high temperatures and electrical stress, but remain stable below 300 K.

2000-02-01

329

Grain mantles: The impact on grain evolution and selective extinction

The authors have studied (Al{sub x}Ga{sub 1{minus}x}){sub 0.5}In{sub 0.5}P doped with tellurium using deep level transient spectroscopy and associated electrical measurements. Several defect states are observed in the upper half of the band gap, that are believed to be intrinsic to the alloy system as well as related to the tellurium donors. Defects observed at measurement temperatures above 390 K exhibit a hysteretic behavior. The observed spectra depend on the biasing conditions applied to the Schottky diode during cooling. The hysteretic behavior suggests the existence of different defect configurations, which can be accessed under conditions of high temperatures and electrical stress, but remain stable below 300 K.

2000-02-01

330

Free-electron laser driven by the LBNL laser-plasma accelerator

Depletion studies are used to infer the presence of mantles and to constrain grain evolutionary models in the diffuse interstellar medium. The presence of these mantles appears to be important in the evolution of the grains inside diffuse as well as dense clouds. In dense clouds where the element-to-element abundances sometimes differ from those found in diffuse clouds, empirical relationships are starting to emerge between gas abundances and various types of peculiar selective extinction. These peculiar extinction curves may be the results of nonvolatile mantle formation on grain cores or may reflect chemical differences due to variations in the intrinsic metalicity from one cloud to another. A simple model of the time evolution of a parcel of gas and dust as observed by the depletion of two elements is presented. Different studies of grain evolution and selective extinction are discussed and compared.

1989-12-01

331

Fabrication and characterization of low aberration micrometer-sized electron lenses

A design of a compact free-electron laser (FEL), generating ultra-fast, high-peak flux, XUV pulses is presented. The FEL is driven by ahigh-current, 0.5 GeV electron beam from the Lawrence Berkeley National Laboratory (LBNL) laser-plasma accelerator, whose active acceleration length is only a few centimeters. The proposed ultra-fast source (~;;10 fs) would be intrinsically temporally synchronized to the drive laser pulse, enabling pump-probe studies in ultra-fast science. Owing to the high current (>10 kA) of the laser-plasma-accelerated electron beams, saturated output fluxes are potentially greater than 10^13 photons/pulse. Devices based both on self-amplified spontaneous emission and high-harmonic generated input seeds, to reduce undulator length and fluctuations, are considered.

2008-08-04

332

British Library Electronic Table of Contents (United Kingdom)

Intrinsic spherical aberrations of electron lenses have been the major resolution limiting factor in electron microscopes for several decades. While effective correctors have recently been implemented, an alternative to correct these aberrations is to circumvent them by scaling down lens dimensions by several orders of magnitude. We have fabricated electrostatic lenses exhibiting one micrometer diameter apertures and evaluated their beam forming properties against predictions from numerical ray tracing simulations. It turns out that it is routinely possible to shape a paraxial low-energy electron beam by such micron-sized lenses. Beam profiles have been measured both at a distant detector as well as in a plane close to the lens. It is shown that the lens can form a parallel beam extending ...

2010-01-01

333

Effects of stearic acid on the injection molding of alumina

EEMD method and WNN for fault diagnosis of locomotive roller bearings

This research investigated the influence of stearic acid on the injection molding of alumina, by varying the concentration of stearic acid. The interaction between stearic acid and alumina was identified using transmission infrared spectroscopy, indicating stearic acid preferentially adsorbed onto the alumina powder surface. In addition to slightly modifying the intrinsic binder viscosity, stearic acid adsorbed onto the powder surface changed the flow behavior of the mixtures from dilatant flow to pseudoplastic flow at low temperatures. As a result, inhomogeneous distribution of binder in the injection-molded parts was minimized with increased stearic acid concentration. However, the possibility of forming bubbles in the mixtures arising from vaporization of stearic acid was enhanced. Additionally, the binder burnout temperature range was broadened with the increase of stearic acid concentration.

1995-10-01

334

British Library Electronic Table of Contents (United Kingdom)

The ensemble empirical mode decomposition (EEMD) can overcome the mode mixing problem of the empirical mode decomposition (EMD) and therefore provide more precise decomposition results. Wavelet neural network (WNN) possesses the advantages of both wavelet transform and artificial neural networks. This paper combines the merits of EEMD and WNN to propose an automated and effective fault diagnosis method of locomotive roller bearings. First, the vibration signals captured from the locomotive roller bearings are preprocessed by EEMD method and intrinsic mode functions (IMFs) are produced. Second, a kurtosis based method is presented and used to select the sensitive IMF. Third, time- and frequency-domain features are extracted from the sensitive IMF, its frequency spectrum and its envelope spe...

2011-01-01

335

Cyclic Spectral Analysis of Radio Pulsars

Cluster Geometry & Inclinations from Deprojection Uncertainties

Cyclic spectral analysis is a signal processing technique designed to deal with stochastic signals whose statistics vary periodically with time. Pulsar radio emission is a textbook example of this signal class, known as cyclostationary signals. In this paper, we discuss the application of cyclic spectral analysis methods to pulsar data, and compare the results with the traditional filterbank approaches used for almost all pulsar observations to date. In contrast to standard methods, the cyclic spectrum preserves phase information of the radio signal. This feature allows us to determine the impulse response of the interstellar medium and the intrinsic, unscattered pulse profile directly from a single observation. We illustrate these new analysis techniques using real data from an observation of the millisecond pulsar B1937+21.

2011-01-01

336

Chemistry of polynuclear transition-metal complexes in ionic liquids.

{The determination of cluster masses is a complex problem that would be aided by information about the cluster shape and orientation (along the line-of-sight).} {It is in this context, that we have developed a scheme for identifying the intrinsic morphology and inclination of a cluster, by looking for the signature of the true cluster characteristics in the inter-comparison of the different deprojected emissivity profiles (that all project to the same X-ray brightness distribution) and by using SZe data when available.} {We deproject the cluster X-ray surface brightness profile under the assumptions of four different geometry and inclination configurations for the observed system; these 4 configurations correspond to four extreme geometry+inclination scenarios. The deprojection in question is performed by the non-parametric algorithm DOPING. The formalism is tested with model systems and then is applied to a sample of 24 clusters. While the shape determination is ...

2008-01-01

337

Black hole radiation in Bose-Einstein condensates

Transition-metal chemistry in ionic liquids (IL) has achieved intrinsic fascination in the last few years. The use of an IL as environmental friendly solvent, offers many advantages over traditional materials synthesis methods. The change from molecular to ionic reaction media leads to new types of materials being accessible. Room-temperature IL have been found to be excellent media for stabilising transition-metal clusters in solution and to crystallise homo- and heteronuclear transition-metal complexes and clusters. Furthermore, the use of IL as solvent provides the option to replace high-temperature routes, such as crystallisation from the melt or gas-phase deposition, by convenient room- or low-temperature syntheses. Inorganic IL composed of alkali metal cations and polynuclear transition-metal cluster anions are also known. Each of these areas will be discussed briefly in this contribution. PMID:21743925

2011-07-11

338

Bioavailability of copper to rats from various foodstuffs and in the presence of different carbohydrates

We study the phonon fluxes emitted when the condensate velocity crosses the speed of sound, i.e., in backgrounds which are analogue to that of a black hole. We focus on elongated one dimensional condensates, and on stationary flows. Our theoretical analysis and numerical results are based on the Bogoliubov-de-Gennes equation without any further approximation. The spectral properties of the fluxes and of the long distance density-density correlations are obtained, with and without an initial temperature. In realistic conditions, we show that the condensate temperature dominates the fluxes, and thus hides the presence of the spontaneous emission (the Hawking effect). We also explain why the temperature amplifies the long distance correlations which are intrinsic to this effect. This confirms that the correlations pattern offers a neat signature of the Hawking effect. Optimal conditions to observe the pattern are discussed.

2009-01-01

339

Are Stars with Planets Polluted?

Copper bioavailability was studied in rats using an extrinsic Cu label. Copper absorption from sunflower seeds (46%), peanuts (41%), cooked shrimp (50%), and cooked beef (40%) was as good or better than copper sulfate (46%). Copper from plant foods (sunflower seeds, garbanzo beans, peanuts) was absorbed equally as well as copper from animal foods (beef, shrimp, chicken liver), 39 +/- 7% vs 43 +/- 7%, P greater than 0.05. There was no significant difference in percentage Cu absorption between intrinsically labeled chicken liver and extrinsically labeled chicken liver. In a second experiment, Cu absorption was measured in the presence of glucose, fructose, sucrose, or cornstarch. There were no significant differences in Cu absorption due to different carbohydrates in a single meal.

1988-01-01

340

Analysis of heterogeneous cell populations: A density-based modeling and identification framework

We compare the metallicities of stars with radial velocity planets to the metallicity of a sample of field dwarfs. We confirm recent work indicating that the stars-with-planet sample as a whole is iron rich. However, the lowest mass stars tend to be iron poor, with several having [Fe/H]0.48) that contributes to but does not explain the mass-metallicity trend in the stars-with-planets sample. We use Monte Carlo models to show that adding an average of 6.5 Earth masses of iron to each star can explain both the mass-metallicity and the age-metallicity relations of the stars-with-planets sample. However, for at least one star, HD 38529, there is good evidence that the bulk metallicity is high. We conclude that the observed metallicities and metallicity trends are the result of the interaction of three effects; accretion of about 6 Earth masses of iron rich material, selection effects, and in some cases, high intrinsic metallicity.

2002-01-01

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341

British Library Electronic Table of Contents (United Kingdom)

Abstract: In many biological processes heterogeneity within clonal cell populations is an important issue. One of the most striking examples is a population of cancer cells in which after a common, identical death signal some cells die whereas others survive. The reason for this heterogeneity is intrinsic and extrinsic noise. In this paper we present a mechanistic multi-scale modeling framework for cell populations, in which the dynamics of every individual cell is captured by a parameter dependent stochastic differential equation (SDE). Heterogeneity among individual cells is accounted for by differences in parameter values, modeling extrinsic influences. Based on the statistical properties of the extrinsic noise and the SDE model for the individual cell, a partial differential equation (...

2011-01-01

342

An efficient quantum secure direct communication scheme with authentication

An Intrinsic Approach to Forces in Magnetoelectric Media

In this paper an efficient quantum secure direct communication (QSDC) scheme with authentication is presented, which is based on quantum entanglement and polarized single photons. The present protocol uses Einstein-Podolsky-Rosen (EPR) pairs and polarized single photons in batches. A particle of the EPR pairs is retained in the sender's station, and the other is transmitted forth and back between the sender and the receiver, similar to the ``ping-pong'' QSDC protocol. According to the shared information beforehand, these two kinds of quantum states are mixed and then transmitted via a quantum channel. The EPR pairs are used to transmit secret messages and the polarized single photons used for authentication and eavesdropping check. Consequently, because of the dual contributions of the polarized single photons, no classical information is needed. The intrinsic efficiency and total efficiency are both 1 in this scheme as almost all of the instances are useful and ...

2007-07-01

343

Absolute values of three neutrino masses from atmospheric mixing and an ansatz for the mixing-matrix elements

This paper offers a conceptually straightforward method for the calculation of stresses in polarisable media based on the notion of a drive form and its property of being closed in spacetimes with symmetry. After an outline of the notation required to exploit the powerful exterior calculus of differential forms, a discussion of the relation between Killing isometries and conservation laws for smooth and distributional drive forms is given. Instantaneous forces on isolated spacetime domains and regions with interfaces are defined, based on manifestly covariant equations of motion. The remaining sections apply these notions to media that sustain electromagnetic stresses, with emphasis on homogeneous magnetoelectric material. An explicit calculation of the average pressure exerted by a monochromatic wave normally incident on a homogeneous, magnetoelectric slab in vacuo is presented and the concluding section summarizes how this pressure depends on the parameters in the magnetoelectric ...

2008-01-01

344

A facile process for preparing superhydrophobic films with surface-modified SiO2/nylon 6,6 nanocomposite

Using data from atmospheric neutrino mixing, and a simple functional form for mixing angles, the absolute values of three neutrino masses are calculated: $m_3\\cong 5.37\\times 10^{-2} eV$, $m_2\\cong 1.94\\times 10^{-2} eV$, $m_1\\cong 1.46\\times 10^{-2} eV$. The quantities relevant for solar neutrino mixing are calculated: $(m_2^2-m_1^2) \\cong 1.63\\times 10^{-4} eV^2$, with non-maximal mixing $\\tan^2\\theta_\\sol \\cong 0.56$. The analysis gives a suggestion of a dynamical origin for the empirical, large CP-violating phase associated with an intrinsically, very small mixing angle in the quark sector.

2003-01-01

345

British Library Electronic Table of Contents (United Kingdom)

A superhydrophobic SiO2/nylon 6,6 nanocomposite coating was fabricated by a facile casting process. Compared with the intrinsically hydrophilic pure nylon 6,6, the as-prepared coating displayed a superhydrophobic property in the pH range from 1 to 14. It was also found that the resulting water contact angles of the as-prepared surface were always larger than 159?, but the sliding angles had an obvious decrease from about 33.4??1? as the content of SiO2 nanoparticles increased. In addition, a transition from the transitional superhydrophobic state between Wenzel?s and Cassie?s state to the Cassie?s state was observed.

2011-01-01

346

The spoIIE homolog of Epulopiscium sp. type B is expressed early in intracellular offspring development.

The Phenomics and Expression Quantitative Trait Locus Mapping of Brain Transcriptomes Regulating Adaptive Divergence in Lake Whitefish Species Pairs (Coregonus sp.)

Epulopiscium sp. type B is an enormous intestinal symbiont of the surgeonfish Naso tonganus. Intracellular offspring production in Epulopiscium shares features with endospore formation. Here, we characterize the spoIIE homolog in Epulopiscium. The timing of spoIIE gene expression and presence of interacting partners suggest that the activation of ?(F) occurs early in Epulopiscium offspring development. PMID:21398534

2011-03-11

347

TGFB1-Induced Extracellular Expression of TGFBIp and Inhibition of TGFBIp Expression by RNA Interference in a Human Corneal Epithelial Cell Line

We used microarrays and a previously established linkage map to localize the genetic determinants of brain gene expression for a backcross family of lake whitefish species pairs (Coregonus sp.). Our...Full Text Available

2008-09-01

348

Regulation of expression of a soybean storage protein subunit gene. Progress report

Purpose.To report the increased production of extracellular transforming growth factor β-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction...Full Text Available

2011-02-01

349

Quox-1, a quail homeobox gene expressed in the embryonic central nervous system, including the forebrain.

We have found that the methionine repression of the ..beta..-subunit gene expression is not due to degradation of the ..beta..-subunit but is due to an effect on synthesis of the ..beta..-subunit. The effect of methionine on the synthesis of the ..beta..-is due to an inhibition of ..beta..-subunit mRNA synthesis. 3 references, 1 figure.

1984-04-23

350

Pteromalus puparum venom impairs host cellular immune responses by decreasing expression of its scavenger receptor gene.

This paper reports the cloning and sequencing of a quail homeobox-containing gene, Quox-1, and its expression pattern in embryos from 3 to 6 days (E3 to E6) of development as determined by in situ hybridization....Full Text Available

1991-03-15

351

Protein expression in white spot syndrome virus infected Penaeus monodon fabricius.

Insect host/parasitoid interactions are co-evolved systems in which host defenses are balanced by parasitoid mechanisms to disable or hide from host immune effectors. Although there is a rich literature on these systems, parasitoid immune-disabling mechanisms have not been fully elucidated. Here we report on a newly discovered immune-disabling mechanism in the Pieris rapae/Pteromalus puparum host/parasitoid system. Because venom injections and parasitization suppresses host phagocytosis, we turned attention to the P. rapae scavenger receptor (Pr-SR), posing the hypothesis that P. puparum venom suppresses expression of the host Pr-SR gene. To test our hypothesis, we cloned a full-length cDNA of the Pr-SR. Multiple sequences alignment showed the deduced amino acid sequence of Pr-SR is similar to scavenger receptors of other lepidopterans. Bacterial and bead injections induced Pr-SR mRNA and protein expression, which peaked at 4h post-bead ...

2011-07-22

352

Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells

White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart tissue and two new ...

2005-06-01

353

Population-Level Retrospective Study of Neurologically Expressed Disorders in Ruminants before the Onset of Bovine Spongiform Encephalopathy (BSE) in Belgium, a BSE Risk III Country

This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60 min of exposure...Full Text Available

2009-05-06

354

Plasmids for the cloning and expression of full-length double-stranded cDNAs under control of the SV40 early or late gene promoter.

A retrospective epidemiological study (n = 7,875) of neurologically expressed disorders (NED) in ruminants before the onset of the bovine spongiform encephalopathy epidemic...Full Text Available

2005-02-01

355

Open Reading Frame all0601 from Anabaena sp. Strain PCC 7120 Represents a Novel Gene, cnaT, Required for Expression of the Nitrate Assimilation nir Operon

Okayama and Berg (1) have recently described a technique for the high efficiency cloning of full-length dscDNAs. We have constructed eukaryotic expression vectors compatible both with this technique...Full Text Available

1983-10-25

356

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli

Expression of the nitrate assimilation nir operon in the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 requires the action of both the...Full Text Available

2003-09-01

357

Infinitesimal symmetries and conservation laws of the DNLSE hierarchy and the Noether's theorem

BackgroundThe large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity...Full Text Available

358

Hypoxia down-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing

The hierarchy of integrable nonlinear equations associated with the quadratic bundle is considered. The expressions for the solution of linearization of these equations and their conservation law in the terms of solutions of corresponding Lax pairs are found. It is shown for the first member of the hierarchy that the conservation law is connected with the solution of linearized equation due to the Noether's theorem. The local hierarchy and three nonlocal ones of the infinitesimal symmetries and conservation laws explicitly expressed through the variables of the nonlinear equations are derived. (author)

2007-08-15

359

Forkhead Box Transcription Factor FOXO3a Regulates Estrogen Receptor Alpha Expression and Is Repressed by the Her-2/neu/Phosphatidylinositol 3-Kinase/Akt Signaling Pathway

sFlt-1 (soluble Flt-1) potently inhibits angiogenesis by binding extracellularly to VEGF (vascular endothelial growth factor). In the present paper, we report that hypoxia down-regulates sFlt-1 expression...Full Text Available

2011-06-01

360

Elimination of L-A double-stranded RNA virus of Saccharomyces cerevisiae by expression of gag and gag-pol from an L-A cDNA clone.

The expression status of the estrogen receptor alpha (ERα) and that of the epidermal growth factor receptor Her-2/neu frequently correlate inversely in breast cancers. While ERα-dependent...Full Text Available

2004-10-01

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361

Distribution and threshold expression of the tRNA(Lys) mutation in skeletal muscle of patients with myoclonic epilepsy and ragged-red fibers (MERRF).

We report that expression of a nearly full-length cDNA clone of the L-A double-stranded RNA virus causes virus loss in a wild-type strain of Saccharomyces cerevisiae. We show that in this system exclusion...Full Text Available

1993-05-01

362

Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

We investigated the distribution and expression of mutant mtDNAs carrying the A-to-G mutation at position 8344 in the tRNA(Lys) gene in the skeletal muscle of four patients with myoclonus epilepsy and...Full Text Available

1992-12-01

363

Context-Driven Cocaine-Seeking in Abstinent Rats Increases Activity-Regulated Gene Expression in the Basolateral Amygdala and Dorsal Hippocampus Differentially following Short and Long Periods of Abstinence

The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and...Full Text Available

364

Communicating Mathematics on the Internet: Synchronous and Asynchronous Tools

In this study, the expression patterns of zif268 and arc were investigated in the basolateral amygdala (BLA) and dorsal hippocampal (dHPC) subregions during context-induced...Full Text Available

2010-10-13

365

British Library Electronic Table of Contents (United Kingdom)

Communicating and collaborating online are becoming common requirements in education. The specialized notations and symbols necessary for some content areas where mathematical expressions are part of the conversation require that users select appropriate communication tools. The purpose of this article is to describe tools that can be used for online, asynchronous or synchronous communication involving mathematical expressions.

2011-01-01

366

Calculation of the imaginary part of the heavy ion potential

Calculation of the imaginary part of the heavy ion potential

The paper contains a numerical evaluation of the expressions for the absorptive potential in heavy ion reactions given earlier. With a standard folding expression for the real part of the ion-ion potential general good agreement is found with experimental data for the angular distributions of elastic and inelastic scattering. Special interest is attached to the case of /sup 16/O + /sup 28/Si where the calculated imaginary potential is very small at low bombarding energies.

1983-09-19

367

Attenuation of oxidant-induced lung injury by 21-aminosteroids (lazaroids): correlation with the mRNA expression for E-selectin, P-selectin, ICAM-1, and VCAM-1.

The paper contains a numerical evaluation of the expressions for the absorptive potential in heavy ion reactions given earlier. With a standard folding expression for the real part of the ion-ion potential general good agreement is found with experimental data for the angular distributions of elastic and inelastic scattering. Special interest is attached to the case of "1"6O + "2"8Si where the calculated imaginary potential is very small at low bombarding energies. (orig.).

368

A Nonparametric Mean-Variance Smoothing Method to Assess Arabidopsis Cold Stress Transcriptional Regulator CBF2 Overexpression Microarray Data

We compared the effects of treatment with methylprednisolone or the 21-aminosteroids, U-74389 and U-74006F (Tirilizad mesylate), on hyperoxic lung injury and the associated expression of mRNA for several...Full Text Available

1994-12-01

369

Thymidylate synthase expression and genotype have no major impact on the clinical outcome of colorectal cancer patients treated with 5-fluorouracil

Microarray is a powerful tool for genome-wide gene expression analysis. In microarray expression data, often mean and variance have certain relationships. We present a non-parametric mean-variance smoothing...Full Text Available

370

British Library Electronic Table of Contents (United Kingdom)

Background and objectives.Thymidylate synthase (TS) expression levels appear to be related to response to 5-fluorouracil-(5-FU)-based chemotherapy in colorectal cancer (CRC) patients. Three polymorphisms have been proposed as modulators of TS expression: a tandemly repeated sequence (2R/3R) in the 5? UTR, a SNP (G > C) within the 3R allele and a 6 bp deletion in the 3' UTR.To evaluate the influence of TS expression and polymorphisms on clinical outcome of 5-FU-treated patients we performed a comprehensive genetic analysis on 63 CRC patients.Methods.TS expression levels were analyzed in normal and tumor tissues. TS coding sequence and UTR polymorphisms were investigated on DNA from normal tissue. LOH analysis was performed to determine tumor genotype.Results.A difference in disease-free sur...

2011-01-01

371

Functional expression of anti-hepatitis B virus (HBV) preS2 antigen scFv by cspA promoter system in Escherichia coli and application as a recognition molecule for single-walled carbon nanotube (SWNT) field effect transistor (FET)

British Library Electronic Table of Contents (United Kingdom)

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in ...

2010-01-01

372

Cotton plants expressing CYP6AE14 double-stranded RNA show enhanced resistance to bollworms

British Library Electronic Table of Contents (United Kingdom)

RNA interference (RNAi) plays an important role in regulating gene expression in eukaryotes. Previously, we generated Arabidopsis and tobacco plants expressing double-stranded RNA (dsRNA) targeting a cotton bollworm (Helicoverpa armigera) P450 gene, CYP6AE14. Bollworms fed on transgenic dsCYP6AE14 plants showed suppressed CYP6AE14 expression and reduced growth on gossypol-containing diet (Mao et?al., in Nat Biotechnol 25: 1307?1313, 2007). Here we report generation and analysis of dsRNA-expressing cotton (Gossypium hirsutum) plants. Bollworm larvae reared on T2 plants of the ds6-3 line exhibited drastically retarded growth, and the transgenic plants were less damaged by bollworms than the control. Quantitative reverse-transcription polymerase chain reaction (RT-PCR) showed that the CYP6AE1...

2011-01-01

373

Temperature dependence of the performance of ultraviolet detectors

Temperature dependence of the performance of ultraviolet detectors

We present the results of a comprehensive study of the temperature dependences of the quantum efficiency for ultraviolet detectors based on GaAs, GaP and 4H--SiC Schottky structures, and on Si, GaAs p-n structures. For ultraviolet detectors based on Schottky structures, the quantum efficiency increases with increasing temperature for all photon energies, even including the semiconductor intrinsic absorption region. On the other hand, for ultraviolet detectors based on p-n structures, the quantum efficiency is practically temperature independent in the semiconductor intrinsic absorption region. The change in the quantum efficiency for the GaAs and Si detectors is less than 0.01% per degree. To explain the measurements, a variable trap occupancy model is presented. Subsurface imperfections of the semiconductor cause fluctuations in the profile of the conduction band and the valence band edges. In the presence of an electric field in the ...

2003-08-21

374

Precision Measurements of the Cluster Red Sequence using an Error Corrected Gaussian Mixture Model

We present the results of a comprehensive study of the temperature dependences of the quantum efficiency for ultraviolet detectors based on GaAs, GaP and 4H--SiC Schottky structures, and on Si, GaAs p-n structures. For ultraviolet detectors based on Schottky structures, the quantum efficiency increases with increasing temperature for all photon energies, even including the semiconductor intrinsic absorption region. On the other hand, for ultraviolet detectors based on p-n structures, the quantum efficiency is practically temperature independent in the semiconductor intrinsic absorption region. The change in the quantum efficiency for the GaAs and Si detectors is less than 0.01% per degree. To explain the measurements, a variable trap occupancy model is presented. Subsurface imperfections of the semiconductor cause fluctuations in the profile of the conduction band and the valence band edges. In the presence of an electric field in the ...

2003-08-21

375

The Differentially Expressed Genes by Radiotherapy in the Patients with Uterine Cervix Cancer

The red sequence is an important feature of galaxy clusters and plays a crucial role in optical cluster detection. Measurement of the slope and scatter of the red sequence are affected both by selection of red sequence galaxies and measurement errors. In this paper, we describe a new error corrected Gaussian Mixture Model for red sequence galaxy identification. Using this technique, we can remove the effects of measurement error and extract unbiased information about the intrinsic properties of the red sequence. We use this method to select red sequence galaxies in each of the 13,823 clusters in the maxBCG catalog, and measure the red sequence ridgeline location and scatter of each. These measurements provide precise constraints on the variation of the average red galaxy populations in the observed frame with redshift. We find that the scatter of the red sequence ridgeline increases mildly with redshift, and that the slope decreases with redshift. We also observe ...

2009-07-01

376

Increased oxidative stress and antioxidant expression in mouse keratinocytes following exposure to paraquat

Purpose : To detect differentially expressed genes in the patients with uterine cervical cancer during the radiation therapy. Materials and Methods : In patients with biopsy proven uterine cervical cancer, we took a tumor tissue just before radiation therapy and at 40 minutes after external irradiation of 1.8 Gy. Total RNAs isolated from non-irradiated and irradiated tumor tissue samples were analyzed using the differential-display reverse transcription-polymerase chain reaction (DDRT-PCR). Complementary DNA (cDNA) fragments corresponding to differentially expressed messenger RNAs(mRNAs) were eluted, and cloned. The differential expression of the corresponding mRNAs was confirmed by reverse northern blot. Differentially expressed cDNA bands were sequenced. Nucleotide sequence data were analyzed in the Gene Bank and EMBL databases via the BLAST network server to identify homologies to known genes or cDNA ...

2001-12-15

377

Apoptotic pathways as regulators of recombination

Paraquat (1,1'-dimethyl-4,4'-bipyridinium) is a widely used herbicide known to induce skin toxicity. This is thought to be due to oxidative stress resulting from the generation of cytotoxic reactive oxygen intermediates (ROI) during paraquat redox cycling. The skin contains a diverse array of antioxidant enzymes which protect against oxidative stress including superoxide dismutase (SOD), catalase, glutathione peroxidase-1 (GPx-1), heme oxygenase-1 (HO-1), metallothionein-2 (MT-2), and glutathione-S-transferases (GST). In the present studies we compared paraquat redox cycling in primary cultures of undifferentiated and differentiated mouse keratinocytes and determined if this was associated with oxidative stress and altered expression of antioxidant enzymes. We found that paraquat readily undergoes redox cycling in both undifferentiated and differentiated keratinocytes, generating superoxide anion and hydrogen peroxide as well as increased protein oxidation which ...

2008-09-15

378

Modulation of neuronal differentiation by CD40 isoforms

Apoptosis, or programmed cell death (PCD), is a fundamental process that protects organismal integrity. In earlier work, we demonstrated that over-expression of either of two anti-apoptotic members of the BCL-2 family (BCL-2 or BCL-X L could elevate the frequency of radiation-induced mutations at the autosomal TK1 locus in human TK6 lymphoblasts that express wild-type TP53. Ectopic expression of BCL-X L also elevated the frequencies of double-strand break-induced gene conversion. The purpose of this study is to determine if BCL-2 family proteins promote radiation mutagenesis indirectly through their suppression of PCD, or whether the 'pro-mutagenic' function of these proteins can be separated from their anti-apoptotic function. We developed stable transfectants of TK6 cells that express a mutated form of BCL-X L with a single amino acid substitution in the BH1 domain that is known to interfere with the ...

2003-08-17

379

Vascular ATP-sensitive potassium channels are over-expressed and partially regulated by nitric oxide in experimental septic shock

Neuron differentiation is a complex process involving various cell-cell interactions, and multiple signaling pathways. We showed previously that CD40 is expressed and functional on mouse and human neurons. In neurons, ligation of CD40 protects against serum withdrawal-induced injury and plays a role in survival and differentiation. CD40 deficient mice display neuron dysfunction, aberrant neuron morphologic changes, and associated gross brain abnormalities. Previous studies by Tone and colleagues suggested that five isoforms of CD40 exist with two predominant isoforms expressed in humans: signal-transducible CD40 type I and a C-terminal truncated, non-signal-transducible CD40 type II. We hypothesized that differential expression of CD40 isoform type I and type II in neurons may modulate neuron differentiation. Results show that adult wild-type, and CD40"-"/"- deficient mice predominantly express CD40 ...

2008-05-02

380

British Library Electronic Table of Contents (United Kingdom)

Purpose To study the activation and expression of vascular (aorta and small mesenteric arteries) potassium channels during septic shock with or without modulation of the NO pathway. Methods Septic shock was induced in rats by peritonitis. Selective inhibitors of vascular KATP (PNU-37883A) or BKCa [iberiotoxin (IbTX)] channels were used to demonstrate their involvement in vascular hyporeactivity. Vascular response to phenylephrine was measured on aorta and small mesenteric arteries mounted on a wire myograph. Vascular expression of potassium channels was studied by PCR and Western blot, in the presence or absence of 1400W, an inducible NO synthase (iNOS) inhibitor. Aortic activation of the transcriptional factor nuclear factor-kappaB (NF-?B) was assessed by electrophoretic mobility shift as...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Tissue-specific expression of Fgfr2b and Fgfr2c isoforms, Fgf10 and Fgf9 in the developing chick mandible

British Library Electronic Table of Contents (United Kingdom)

SummaryExperimental evidence has demonstrated the importance of FGF signalling in morphogenesis of the mandibular processes. FGFs transmit their signals through four tyrosine kinase transmembrane receptors (FGFRs). Alternative splicing in FGFRs including FGFR2 generates different isoforms that exhibit different ligand-specificities, exclusive tissue distributions and specific biological functions. Despite extensive information regarding the isoform-specific patterns of expression Fgfr2c and Fgfr2b during morphogenesis of many organs, a comparative analysis of these specific isoforms in the chick mandible has not been reported. To better understand the function of FGFR2 in mandibular morphogenesis, we have analysed the expression Fgfr2b, Fgfr2c and their putative ligands Fgf10 and Fgf9, in ...

2006-01-01

382

The Art of Articulation: Political Engagement and Social Movements in the Making among Young Adults in Multicultural Settings in Norway

British Library Electronic Table of Contents (United Kingdom)

Participation in conventional politics, such as elections, membership of organizations and political parties, is relatively low among young adults of ethnic minority background. Instead, engagement seems to find its way through aesthetic and other expressive channels of influence drawing on new technologies, impulses from transnational youth culture traditions, and both street riots and less conflictual actions. The aim of this article is to grasp a potential social movement in the making, by exploring the processes of articulation through which young people from immigrant families in Norway express their political engagement. The phenomenon of articulation is understood as a process comprising three steps or modes: (i) becoming conscious; (ii) generating expressions and (iii) collective a...

2011-01-01

383

SEPT9_i1 and genomic instability: Mechanistic insights and relevance to tumorigenesis

British Library Electronic Table of Contents (United Kingdom)

Abstract Septins are highly conserved cytoskeletal GTP-binding proteins implicated in numerous cellular processes from apoptosis to vesicle trafficking. Septins have been associated with leukemia and solid tumor malignancies, including breast, ovarian, and prostate. We previously reported that high SEPT9_i1 expression in human mammary epithelial cell lines (HMECs) led to malignant cellular phenotypes such as increased cell proliferation, invasiveness, motility, and genomic instability. Our goal here was to better understand how SEPT9_i1 expression might contribute to genomic instability and malignant progression. First, we confirmed that even transient expression of SEPT9_i1 was sufficient to increase aneuploidy in HMECs. We then analyzed SEPT9_i1 by immunoprecipitation and immunofluoresce...

2011-01-01

384

Regulation of C-type natriuretic peptide expression

British Library Electronic Table of Contents (United Kingdom)

C-type natriuretic peptide (CNP) is a member of the small family of natriuretic peptides that also includes atrial natriuretic peptide (ANP) and brain, or B-type natriuretic peptide (BNP). Unlike them, it performs its major functions in an autocrine or paracrine manner. Those functions, mediated through binding to the membrane guanylyl cyclase natriuretic peptide receptor B (NPR-B), or by signaling through the non-enzyme natriuretic peptide receptor C (NPR-C), include the regulation of endochondral ossification, reproduction, nervous system development, and the maintenance of cardiovascular health. To date, the regulation of CNP gene expression has not received the attention that has been paid to regulation of the ANP and BNP genes. CNP expression in vitro is regulated by TGF-b and recepto...

2011-01-01

385

On the curvature in logarithmic plots of rate coefficients for chemical reactions

Intermediate-conductance calcium-activated potassium channels participate in neurovascular coupling

In terms of the reduced potential energy barrier ? = ?uTS/kT, the rate coefficients for chemical reactions are usually expressed as proportional to e-?. The coupling between vibrational modes of the medium to the reaction coordinate leads to a proportionality of the regularized gamma function of Euler Q(a,?) = ?(a,?)/?(a), with a being the number of modes coupled to the reaction coordinate. In this work, the experimental rate coefficients at various temperatures for several chemical reactions were fitted to the theoretical expression in terms of Q(a,?) to determine the extent of its validity and generality. The new expression affords lower deviations from the experimental points in 29 cases out of 38 and it accounts for the curvature in the logarithmic plots of rate coefficients versus inverse temperature. In the absence of tunneling, conventional theories predict the curvature of these plots to be identically zero.

2011-05-06

386

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND AND PURPOSE Controlling vascular tone involves K+ efflux through endothelial cell small- and intermediate-conductance calcium-activated potassium channels (KCa2.3 and KCa3.1, respectively). We investigated the expression of these channels in astrocytes and the possibility that, by a similar mechanism, they might contribute to neurovascular coupling. EXPERIMENTAL APPROACH Transgenic mice expressing enhanced green fluorescent protein (eGFP) in astrocytes were used to assess KCa2.3 and KCa3.1 expression by immunohistochemistry and RT-PCR. KCa currents in eGFP-positive astrocytes were determined in situ using whole-cell patch clamp electrophysiology. The contribution of KCa3.1 to neurovascular coupling was investigated in pharmacological experiments using electrical field stimulatio...

2011-01-01

387

Interlattice displacements and elastic constants of the A-15 compounds V_3Si, V_3Ge and Nb_3Sn

Interlattice displacements and elastic constants of the A-15 compounds V3Si, V3Ge and Nb3Sn

General expressions for the interlattice displacements of the A-15 structure compounds are obtained in terms of the strain components making use of the deformation theory. The nature of the interlattice displacements of all the 8 atoms in the unit cell is discussed. It is found that the interlattice displacements occur in such a way that the pair of atoms along any linear chain move in opposite directions with equal magnitudes. Expression for the strain energy of these compounds is developed using deformation theory and this is compared with the strain energy expression from continuum theory to obtain the elastic constants. The theoretical values of the elastic constants fairly agree with the experimental values for V_3Si, V_3Ge and Nb_3Sn. (author).

388

Hypomethylation and induction of retinoic acid receptor beta 2 by concurrent action of adenosine analogues and natural compounds in breast cancer cells

General expressions for the interlattice displacements of the A-15 structure compounds are obtained in terms of the strain components making use of the deformation theory. The nature of the interlattice displacements of all the 8 atoms in the unit cell is discussed. It is found that the interlattice displacements occur in such a way that the pair of atoms along any linear chain move in opposite directions with equal magnitudes. Expression for the strain energy of these compounds is developed using deformation theory and this is compared with the strain energy expression from continuum theory to obtain the elastic constants. The theoretical values of the elastic constants fairly agree with the experimental values for V3Si, V3Ge and Nb3Sn.

1978-03-01

389

British Library Electronic Table of Contents (United Kingdom)

DNA methylation is considered as a potential cause of aberrations in regulation of gene expression during carcinogenesis. Therefore, changes in DNA methylation patterns may be targets for chemoprevention. In the present study, we investigated effects of all-trans retinoic acid (ATRA), vitamin D3, and resveratrol alone and in combination with adenosine analogues: 2-chloro-2'-deoxyadenosine (2CdA) and 9-beta-d-arabinosyl-2-fluoroadenine (F-ara-A), on methylation and expression of retinoic acid receptor beta 2 (RARbeta2) in MCF-7 and MDA-MB-231 breast cancer cell lines. Alterations in methylation and expression levels after treatment of cells with the tested compounds were evaluated by methylation-sensitive restriction analysis (MSRA) and real-time PCR, respectively. RARbeta2 promoter in the ...

2010-01-01

390

Gene expression analysis identifies potential biomarkers of phenanthrene in human hepatocytes (HepG2)

British Library Electronic Table of Contents (United Kingdom)

Polycyclic aromatic hydrocarbons (PAHs) are ubiquious in the environment both as natural products and as environmental contaminants. Among PAHs, phenanthrene (PH) that is ubiquitously distributed throughout the environment was subjected in this study. Although environmental distribution and metabolism of PH have been well reported, there are only a few studies examined the expression of mRNA and their functions on PH-induced toxicity. A new paradigm in toxicity screening, toxicogenomic technology represents a useful approach for evaluating the toxic properties of environmental pollutants. In this respect, we elicited the genes which were changed more than 2-fold by analysis of gene expression profiles in human hepatocellular carcinoma (HepG2) cells, exposed to PH by using human oligonucleo...

2011-01-01

391

Food-grade gene expression in lactic acid bacteria

British Library Electronic Table of Contents (United Kingdom)

Abstract In the 1990s, significant efforts were invested in the research and development of food-grade expression systems in lactic acid bacteria (LAB). At this time, Lactococcus lactis in particular was demonstrated to be an ideal cell factory for the food-grade production of recombinant proteins. Steady progress has since been made in research on LAB, including Lactococcus, Lactobacillus and Streptococcus, in the areas of recombinant enzyme production, industrial food fermentation, and gene and metabolic pathway regulation. Over the past decade, this work has also led to new approaches on chromosomal integration vectors and host/vector systems. These newly constructed food-grade gene expression systems were designed with specific attention to self-cloning strategies, food-grade selection...

2011-01-01

392

Expression of suppressor of cytokine signaling 1 and 3 in ligature-induced periodontitis in rats

British Library Electronic Table of Contents (United Kingdom)

Objective: : Evaluate expression of inducible negative regulators of JAK/STAT pathway and their target proteins during the course of ligature-induced experimental periodontal disease in rats. Design: : Rats were sacrificed 07, 15 and 30days after disease induction for histological evaluation of periodontal inflammation and macroscopic analysis of alveolar bone loss. SOCS expression and the activation status of STAT1 and STAT3 were evaluated in gingival biopsies by real time PCR and Western blot. Results: : Ligature-induced model presented significant progressive bone loss from 7 to 30days. Inflammation was evident and similar for 07 and 15days; however, a decrease on severity at the end of the experimental period was observed. There was a significant (p<0.05) increase on SOCS1 and SOCS3 ge...

2011-01-01

393

Expression of Distal-less, dachshund, and optomotor blind in Neanthes arenaceodentata (Annelida, Nereididae) does not support homology of appendage-forming mechanisms across the Bilateria

British Library Electronic Table of Contents (United Kingdom)

The similarity in the genetic regulation of arthropod and vertebrate appendage formation has been interpreted as the product of a plesiomorphic gene network that was primitively involved in bilaterian appendage development and co-opted to build appendages (in modern phyla) that are not historically related as structures. Data from lophotrochozoans are needed to clarify the pervasiveness of plesiomorphic appendage-forming mechanisms. We assayed the expression of three arthropod and vertebrate limb gene orthologs, Distal-less (Dll), dachshund (dac), and optomotor blind (omb), in direct-developing juveniles of the polychaete Neanthes arenaceodentata. Parapodial Dll expression marks pre-morphogenetic notopodia and neuropodia, becoming restricted to the bases of notopodial cirri and to ventral ...

2010-01-01

394

Epigenetic regulation of autosomal gene expression by sex chromosomes

British Library Electronic Table of Contents (United Kingdom)

Males and females display differences in physiology, behaviour and susceptibility to many diseases. Genome-wide transcription profiling studies have uncovered large-scale sex differences in autosomal gene expression in somatic tissues that are thought to underlie such 'sexual dimorphisms'. Because males and females differ genetically mainly in their sex chromosome complement, most sex differences can be traced back to the X and Y chromosomes. Although sex hormones are usually considered the main architects of sexual dimorphisms, recent studies have demonstrated that sex chromosomes can also induce sex differences in somatic gene expression in the absence of hormonal differences. The recent discovery of epigenetic sex differences that are not hormone-induced brings us closer to understandin...

2011-01-01

395

Cyclooxygenase expression and prostaglandin levels in central nervous system tissues during the course of chronic relapsing experimental autoimmune encephalomyelitis (EAE)

British Library Electronic Table of Contents (United Kingdom)

Objective Multiple sclerosis (MS) and its animal counterpart experimental autoimmune encephalomyelitis (EAE) have a major inflammatory component that drives and orchestrates both diseases. One particular group of mediators are the prostaglandins (PGs), which we have previously shown, through quantitation and pharmacological intervention, to be closely involved in the pathology of MS and EAE. The aim of the current study was to determine the expression of the PG-generating cyclooxygenase (COX) enzymes and the profile of PGE2 and PGD2, in selected central nervous system (CNS) tissues, with the development of the chronic relapsing (CR) form of EAE. In particular, the work investigates the possible relationship between the expression of COX isoenzymes and PG levels during the neurological phas...

2011-01-01

396

Aldehyde dehydrogenase (ALDH) 3A1 expression by the human keratocyte and its repair phenotypes

British Library Electronic Table of Contents (United Kingdom)

Transparency is essential for normal corneal function. Recent studies suggest that corneal cells express high levels of so-called corneal crystallins, such as aldehyde dehydrogenase (ALDH) and transketolase (TKT) that contribute to maintaining cellular transparency. Stromal injury leads to the appearance of repair phenotype keratocytes, the corneal fibroblast and myofibroblast. Previous studies on keratocytes from species such as bovine and rabbit indicate that the transformation from the normal to repair phenotype is accompanied by a loss of corneal crystallin expression, which may be associated with loss of cellular transparency. Here we investigated if a similar loss occurs with human keratocyte repair phenotypes. Human corneal epithelial cells were collected by scraping and keratocytes...

2006-01-01

397

A Hybrid Multi Objective Particle Swarm Optimization Method to Discover Biclusters in Microarray Data

var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2

In recent years, with the development of microarray technique, discovery of useful knowledge from microarray data has become very important. Biclustering is a very useful data mining technique for discovering genes which have similar behavior. In microarray data, several objectives have to be optimized simultaneously and often these objectives are in conflict with each other. A Multi Objective model is capable of solving such problems. Our method proposes a Hybrid algorithm which is based on the Multi Objective Particle Swarm Optimization for discovering biclusters in gene expression data. In our method, we will consider a low level of overlapping amongst the biclusters and try to cover all elements of the gene expression matrix. Experimental results in the bench mark database show a significant improvement in both overlap among biclusters and coverage of elements in the gene expression matrix.

2009-01-01

398

cDNA-AFLP analysis reveals differential gene expression in compatible interaction of wheat challenged with Puccinia striiformis f. sp. tritici

BackgroundThe pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell...Full Text Available

399

Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space

BackgroundPuccinia striiformis f. sp. tritici is a fungal pathogen causing stripe rust, one of the most important wheat diseases worldwide. The...Full Text Available

400

Unraveling gene regulatory networks from time-resolved gene expression data -- a measures comparison study

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

2010-12-01

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401

Ubiquitin over-expression phenotypes and ubiquitin gene molecular misreading during aging in Drosophila melanogaster

BackgroundInferring regulatory interactions between genes from transcriptomics time-resolved data, yielding reverse engineered gene regulatory networks, is of paramount importance...Full Text Available

402

Transplantation of allogeneic T cells alters iron homeostasis in NOD/SCID mice

Molecular Misreading (MM) is the inaccurate conversion of genomic information into aberrant proteins. For example, when RNA polymerase II transcribes a GAGAG motif it synthesizes at low frequency RNA...Full Text Available

403

Transcriptome Profiling of a Toxic Dinoflagellate Reveals a Gene-Rich Protist and a Potential Impact on Gene Expression Due to Bacterial Presence

Iron overload is common in patients undergoing allogeneic hematopoietic cell transplantation (HCT), but the mechanisms leading to overload are unknown. Here, we determined iron levels and the expression...Full Text Available

2009-02-19

404

The p14 FAST Protein of Reptilian Reovirus Increases Vesicular Stomatitis Virus Neuropathogenesis?

BackgroundDinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear...Full Text Available

405

The Melanocortin 3 Receptor: A Novel Mediator of Exercise-Induced Inflammation Reduction in Postmenopausal Women?

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

2009-01-01

406

The Differential Expression of Aqueous Soluble Proteins in Breast Normal and Cancerous Tissues in Relation to Stage and Grade of Patients

The purpose of this study was to determine whether resistance exercise training-induced reductions in inflammation are mediated via melanocortin 3 receptor expression in obese (BMI 32.7 ± 3.7)...Full Text Available

407

Temporal and Tissue-Specific Patterns of Pon3 Expression in Mouse: In situ Hybridization Analysis

Breast cancer is a leading cause of female deaths worldwide. In Malaysia, it is the most common form of female cancer while Infiltrating ductal carcinoma (IDC) is the most common form of breast cancer....Full Text Available

2010-01-01

408

Targeted Deletion of Somatotroph Insulin-Like Growth Factor-I Signaling in a Cell-Specific Knockout Mouse Model

PON3 is a member of the paraoxonase gene family that includes PON1 and PON2. For example, PON3 and PON1 share...Full Text Available

2010-01-01

409

Syk Tyrosine Kinase Acts as a Pancreatic Adenocarcinoma Tumor Suppressor by Regulating Cellular Growth and Invasion

The role of IGF-I in the negative regulation of GH expression and release is demonstrated by in vitro and in vivo models; however, the targets and mechanisms of IGF-I...Full Text Available

2010-05-01

410

Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica

We have identified the nonreceptor tyrosine kinase syk as a marker of differentiation/tumor suppressor in pancreatic ductal adenocarcinoma (PDAC). Syk expression is lost in poorly differentiated PDAC...Full Text Available

2009-12-01

411

Spontaneous expression of the interleukin 2 receptor gene and presence of functional interleukin 2 receptors on T lymphocytes in the blood of individuals with active pulmonary sarcoidosis.

BackgroundThe common liver fluke Fasciola hepatica is the agent of a zoonosis with significant economic consequences in livestock production worldwide, and increasing...Full Text Available

412

Reverse Engineering of Gene Regulatory Networks: A Comparative Study

Current concepts of the pathogenesis of sarcoidosis suggest that the expanded numbers of activated T-helper/inducer cells at sites of disease activity result, at least in part, from their proliferation...Full Text Available

1988-09-01

413

Proteomic approach with LCMS-IT-TOF identified an increase of Rab33B after transient focal cerebral ischemia in mice

Reverse engineering of gene regulatory networks has been an intensively studied topic in bioinformatics since it constitutes an intermediate step from explorative to causative gene expression...Full Text Available

2009-01-01

414

Profiling highly conserved microrna expression in recombinant IgG-producing and parental Chinese hamster ovary cells

BackgroundSeveral proteins are known to be markedly expressed in the brain during cerebral ischemia; however, the changes in protein profiles within the ischemic brain after an ischemic...Full Text Available

415

British Library Electronic Table of Contents (United Kingdom)

Abstract MicroRNAs (miRNAs) play important roles in global gene regulation. Researchers in recombinant protein production have proposed miRNAs as biomarkers and cell engineering targets. However, miRNA expression remains understudied in Chinese Hamster Ovary cells, one of the most commonly used host cell systems for therapeutic protein production. To profile highly conserved miRNA expression, we used the miRCURY- miRNA array for screening miRNAs in CHO cells. The selection criteria for further miRNA profiling included positive hybridization signals and experimentally validated predicted regulatory targets. On the basis of screening, we selected 16 miRNAs for quantitative RT-PCR profiling. We profiled miR expression in parental CHO DG44 and CHO K1 cell lines as well as four recombinant DG44...

2011-01-01

416

Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum.

Pathophysiological, Genetic and Gene Expression Features of a Novel Rodent Model of the Cardio-Metabolic Syndrome

We have examined the expression and structure of several genes belonging to two classes of vegetative specific genes of the simple eukaryote, Dictyostelium discoideum. In amebae grown on bacteria, deactivation...Full Text Available

1989-12-11

417

PEG-conjugated PAMAM Dendrimers Mediate Efficient Intramuscular Gene Expression

BackgroundComplex etiology and pathogenesis of pathophysiological components of the cardio-metabolic syndrome have been demonstrated in humans and animal models.Methodology/Principal...Full Text Available

418

Overexpression of bacterial ethylene-forming enzyme gene in Trichoderma reesei enhanced the production of ethylene

Generations 5 and 6 (G5 and G6) poly(amidoamine) (PAMAM) dendrimers have been shown to be highly efficient nonviral carriers in in vitro gene delivery. However, their high toxicity...Full Text Available

419

Once more: gravity is not an entropic force

In order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from Pseudomonas...Full Text Available

420

Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression

We argue that neutron interference experiments and experiments on gravitational bound states of neutron unambiguously disprove entropic origin of gravitation. The criticism expressed in a recent paper [arXiv:1104.4650] concerning our arguments against entropic gravity is shown to be invalid.

2011-01-01

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421

Nonnative Proteins Induce Expression of the Bacillus subtilis CIRCE Regulon

Noncoding RNAs play important roles in various aspects of gene regulation. We have identified 7SK RNA to be enriched in nuclear speckles or interchromatin granule clusters (IGCs), a subnuclear domain...Full Text Available

2010-12-01

422

Neutron stars in massive binary systems. I. Classification and evolution

The chaperone-encoding groESL and dnaK operons constitute the CIRCE regulon of Bacillus subtilis. Both operons are under negative control of the repressor...Full Text Available

1998-06-01

423

Neutral endopeptidase inhibits prostate cancer cell migration by blocking focal adhesion kinase signaling

A study of the joint evolution of the normal- and neutron-star components of massive binaries opens with a classification scheme and the analytic expressions to be applied in Paper II for computer simulation of the observable properties of such systems.

1983-03-01

424

Neurotensin Receptor 1 Is Expressed in Gastrointestinal Stromal Tumors but Not in Interstitial Cells of Cajal

Neutral endopeptidase 24.11 (NEP, CD10) is a cell-surface enzyme expressed by prostatic epithelial cells that cleaves and inactivates neuropeptides implicated in the growth of androgen-independent prostate...Full Text Available

2000-12-01

425

Neonatal E. coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats

Gastrointestinal stromal tumors (GIST) are thought to derive from the interstitial cells of Cajal (ICC) or an ICC precursor. Oncogenic mutations of the KIT or PDGFRA receptor tyrosine kinases are present...Full Text Available

426

Move or Die: the Fate of the Tax Oncoprotein of HTLV-1

Neonatal bacterial infection in rats alters the responses to a variety of subsequent challenges later in life. Here we explored the effects of neonatal bacterial infection on a subsequent drug...Full Text Available

2010-04-19

427

Molecular cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

The HTLV-1 Tax protein both activates viral replication and is involved in HTLV-1-mediated transformation of T lymphocytes. The transforming properties of Tax include altering the expression of select...Full Text Available

428

Modulation of ASK1 expression during overexpression of Trx and HSP70 in stressed fish liver mitochondria

This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...Full Text Available

1989-11-01

429

MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms

Mitochondrial heat shock protein 70 (mtHSP70) is found to play a primary role in cellular defense against physiological stress like exposure to environmental contaminants and helpful in the maintenance...Full Text Available

2009-09-01

430

Mechanism of Na(+)-dependent citrate transport in Klebsiella pneumoniae.

MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences....Full Text Available

2003-08-19

431

Mapping neuropeptide expression by mass spectrometry in single dissected identified neurons from the dorsal ganglion of the nematode Ascaris suum

Citrate transport via CitS of Klebsiella pneumoniae has been shown to depend on the presence of Na+. This transport system has been expressed in Escherichia coli, and uptake of citrate in E. coli membrane...Full Text Available

1992-08-01

432

Mammalian target of rapamycin signaling modulates photic entrainment of the suprachiasmatic circadian clock

We have developed a method for dissecting single neurons from the nematode Ascaris suum, in order to determine their peptide content by mass spectrometry (MS). In this paper,...Full Text Available

2010-07-21

433

Malignant Catarrhal Fever of Cattle Is Associated with Low Abundance of IL-2 Transcript and a Predominantly Latent Profile of Ovine Herpesvirus 2 Gene Expression

Inducible gene expression appears to be an essential event that couples light to entrainment of the master mammalian circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus....Full Text Available

2010-05-05

434

Light-Dependent Regulation of Cyanobacterial Phytochrome Expression

BackgroundMalignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes...Full Text Available

435

Lifespan extension by increased expression of the Drosophila homologue of the IGFBP7 tumour suppressor

A histidine kinase protein (Cph1) with sequence homology and spectral characteristics very similar to those of the plant phytochrome has been recently identified in the cyanobacterium Synechocystis...Full Text Available

2000-01-01

436

Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma

Mammals possess multiple insulin-like growth factor (IGF) binding proteins (IGFBPs), and related proteins, that modulate the activity of insulin/IGF signalling (IIS), a conserved neuroendocrine signalling...Full Text Available

2011-02-01

437

Increased SRF transcriptional activity in human and mouse skeletal muscle is a signature of insulin resistance

... protein is abundant in cytosolic portions of malignant epithelial tissue (Fig. 3A). In contrast, the degree of CCTβ staining in normal epithelial tissue (Fig. 3B) or connective tissues was much weaker ......

438

Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an Avena sativa Plasma Membrane Determinant 1

Insulin resistance in skeletal muscle is a key phenotype associated with type 2 diabetes (T2D) for which the molecular mediators remain unclear. We therefore conducted an expression analysis of human...Full Text Available

2011-03-01

439

Identification of a distant cis-regulatory element controlling pharyngeal arch-specific expression of zebrafish gdf6a/radar

An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target...Full Text Available

1992-01-01

440

Identification of Human Prostate Cancer Stem Cells by ...

Skeletal formation is an essential and intricately regulated part of vertebrate development. Humans and mice deficient in Growth and Differentiation Factor 6 (Gdf6) have numerous...Full Text Available

2010-04-01

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441

Human mitochondrial transcription factor A reduction and mitochondrial dysfunction in Hashimoto's hypothyroid myopathy.

... The basal markers CK5, alpha 6 integrin (CD49f), CD44, and p63 were strongly expressed by the majority of sphere-forming cells. ... p63 p63 PSCA ...

2008-04-01

442

Human intronic enhancers control distinct sub-domains of Gli3 expression during mouse CNS and limb development

BACKGROUND: Mitochondrial changes have been described in muscle tissue in acquired hypothyroidism. Among the molecular mechanisms by which thyroid hormones regulate expression of nuclear genes encoding...Full Text Available

2002-06-01

443

Hsp12.6 Expression Is Inducible by Host Immunity in Adult Worms of the Parasitic Nematode Nippostrongylus brasiliensis

BackgroundThe zinc-finger transcription factor GLI3 is an important mediator of Sonic hedgehog signaling and crucial for patterning of many aspects of the vertebrate body plan. In...Full Text Available

444

Glutamate Receptor-Mediated Restoration of Experience-Dependent Place Field Expansion Plasticity in Aged Rats

Heat shock proteins (Hsp) are a family of stress-inducible molecular chaperones that play multiple roles in a wide variety of animals. However, the roles of Hsps in parasitic nematodes remain largely...Full Text Available

445

Glucocorticoids exacerbate hypoxia induced expression of the pro-apoptotic gene Bnip3 in the developing cortex

Place fields of hippocampal pyramidal cells expand asymmetrically when adult rats repeatedly follow the same route. This behaviorally-induced expression of neuronal plasticity utilizes an NMDA_R-dependent,...Full Text Available

2008-06-01

446

Global depression in gene expression as a response to rapid thermal changes in vent mussels

Neonatal administration of the synthetic glucocorticoid, dexamethasone (DEX) retards brain growth, alters adult behaviors and induces cell death in the rat brain, thereby implicating glucocorticoids...Full Text Available

2007-01-19

447

Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc

Hydrothermal vent mussels belonging to the genus Bathymodiolus are distributed worldwide and dominate communities at shallow Atlantic hydrothermal sites. While organisms inhabiting...Full Text Available

2009-09-07

448

Gene-Environment Interactions and Epigenetic Basis of Human Diseases

Zinc is an essential trace metal ion for growth, but an excess of Zn is toxic and microorganisms express diverse resistance mechanisms. To understand global bacterial responses to excess Zn, we conducted...Full Text Available

2005-02-01

449

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

Most human diseases are related in some way to the loss or gain in gene functions. Regulation of gene expression is a complex process. In addition to genetic mechanisms, epigenetic causes are...Full Text Available

2008-01-01

450

Gene expression profile of rat left ventricles reveals persisting changes following chronic mild exercise protocol: implications for cardioprotection

BackgroundA strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased...Full Text Available

451

From Classical Genetics to Quantitative Genetics to Systems Biology: Modeling Epistasis

BackgroundEpidemiological studies showed that physical exercise, specifically moderate lifelong training, is protective against cardiovascular morbidity and mortality. Most experimental...Full Text Available

452

Feature Selection and Classification of MAQC-II Breast Cancer and Multiple Myeloma Microarray Gene Expression Data

Gene expression data has been used in lieu of phenotype in both classical and quantitative genetic settings. These two disciplines have separate approaches to measuring and interpreting epistasis, which...Full Text Available

2008-03-01

453

Expression of the ?^B-Dependent General Stress Regulon Confers Multiple Stress Resistance in Bacillus subtilis

Microarray data has a high dimension of variables but available datasets usually have only a small number of samples, thereby making the study of such datasets interesting and challenging. In the task...Full Text Available

454

Expression of leukotriene receptors in the rat dorsal root ganglion and the effects on pain behaviors

The alternative sigma factor ς^B of Bacillus subtilis is required for the induction of approximately 100 genes after the imposition of a whole range of stresses and...Full Text Available

1999-07-01

455

Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase

BackgroundLeukotrienes (LTs) belong to the large family of lipid mediators implicated in various inflammatory conditions such as asthma and rheumatoid arthritis. Four distinct types...Full Text Available

456

Expression and purification of a functional human hepatitis B virus polymerase

BackgroundChitin synthase 3a (CHS3a) from Botrytis cinerea (Bc) catalyses the multiple transfer of N-acetylglucosamine (GlcNAc) residues to the...Full Text Available

457

Expression and Redistribution of Cellular Bad, Bax, and Bcl-xL Protein Is Associated with VCD-Induced Ovotoxicity in ...

AIM: To identify a method for efficient large-scale purification of functional hepatitis B virus polymerase (HBV-Pol) without addition of cellular factors.METHODS: Full-length HBV-Pol (843 amino...Full Text Available

2010-12-07

458

Energy balances of OECD countries 1985/1986

... were given daily i.p. injections of either sesame oil (2.5 ml/kg, vehicle control) or VCD dissolved in sesame oil (80 mg/kg, 0.57 mmol/kg) for ... ...

459

Effects of intrauterine undernutrition on hypothalamic Kiss1 expression and the timing of puberty in female rats

Provides standardized energy balance sheets expressed in a common unit of tons of oil equivalent for all OECD countries as well as the regions: OECD total, IEA, North America, Pacific, OECD Europe, EEC and Yugoslavia.

1988-01-01

460

Effects of gradual coronary artery occlusion and exercise training on gene expression in swine heart

Recent studies have suggested that intrauterine undernutrition is closely associated with the pathogenesis of diseases after birth. Perinatal undernutrition is known to disturb the development of reproductive...Full Text Available

2010-03-01

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461

Ectopic Expression of Constitutively Activated RACB in Barley Enhances Susceptibility to Powdery Mildew and Abiotic Stress1

Gradual occlusion (O) of the swine left circumflex coronary artery (LCX) with an ameroid occluder results in complete O within 3 weeks, collateral vessel development, and compensatory hypertrophy....Full Text Available

2007-01-01

462

Dynamin-dependent NMDAR endocytosis during LTD and its dependence on synaptic state

Small RAC/ROP-family G proteins regulate development and stress responses in plants. Transient overexpression and RNA interference experiments suggested that the barley (Hordeum vulgare)...Full Text Available

2005-09-01

463

Divergent Regulation of Angiopoietin-1 and -2, Tie-2, and Thrombospondin-1 Expression by Estrogen in the Baboon Endometrium

BackgroundThe N-methyl-D-aspartate (NMDA)-type glutamate receptor expressed at excitatory glutamatergic synapses is required for learning and memory and is critical for normal brain...Full Text Available

464

Diurnal Variations of Mouse Plasma and Hepatic Bile Acid Concentrations as well as Expression of Biosynthetic Enzymes and Transporters

SUMMARYEstrogen has an important role in the reconstruction of a new vascular network in the endometrium during each menstrual cycle; however, the underlying mechanisms...Full Text Available

2010-05-01

465

Differential interleukin-6/Stat3 signaling as a function of cellular context mediates Ras-induced transformation

BackgroundDiurnal fluctuation of bile acid (BA) concentrations in the enterohepatic system of mammals has been known for a long time. Recently, BAs have been recognized as signaling...Full Text Available

466

Differential expression of Haemophilus parasuis genes in response to iron restriction and cerebrospinal fluid

IntroductionTyrosine phosphorylated signal transducer and activator of transcription 3 (pStat3) is expressed in numerous cancers and is required for mediating tumorigenesis. Autocrine...Full Text Available

2010-01-01

467

Differential Gene Expression in Primary Human Skin Keratinocytes and Fibroblasts in Response to Ionizing Radiation

Haemophilus parasuis is an important opportunistic pathogen in swine of high health status, but to date no proven virulence factors have been described. As virulence factors are known...Full Text Available

2007-07-01

468

Developmental and Light Regulation of Desacetoxyvindoline 4-Hydroxylase in Catharanthus roseus (L.) G. Don.1

Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes...Full Text Available

2009-07-01

469

Developmental Changes in Pituitary Adenylate Cyclase Activating Polypeptide Expression during the Perinatal Period: Possible Role in Fetal Gonadotroph Regulation

The expression of desacetoxyvindoline 4-hydroxylase (D4H), which catalyzes the second to the last reaction in vindoline biosynthesis in Catharanthus roseus, appears to be under complex,...Full Text Available

1998-08-01

470

Decentralized Data Sharing of Tissue Microarrays for Investigative Research in Oncology

Normal reproductive functioning may require secretion of LH independently of FSH. Variation in GnRH pulse frequency and inhibin negative feedback are mechanisms for differential gonadotropin regulation;...Full Text Available

2009-10-01

471

Cutaneous expression of Thy-1 in mycosis fungoides.

Tissue microarray technology (TMA) is a relatively new approach for efficiently and economically assessing protein and gene expression across large ensembles of tissue specimens. Tissue microarray technology...Full Text Available

472

Crystallization and preliminary X-ray analysis of human liver ?-enolase

Dermal dendritic cells from eleven cases of mycosis fungoides (MF) (six patch and five plaque stage), two cases of pre-MF, and five specimens of normal human skin, were characterized immunohistochemically...Full Text Available

1992-12-01

473

Corticosteroid can alter antigen expression on alveolar macrophages.

Enolase is a multifunctional enzyme that plays important roles in many biological and disease processes. α-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by...Full Text Available

474

Coordinated Increased Expression of Cyclooxygenase2 and Nuclear Factor ?B Is a Steady Feature of Urinary Bladder Carcinogenesis

Normal healthy volunteers underwent broncho-alveolar lavage and the cells obtained were cultured for 24 h and 48 h, either alone or in the presence of the corticosteroid, Budesonide. Cell differentials...Full Text Available

1991-09-01

475

Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells.

Objectives. The inescapable relationship between chronic inflammation and carcinogenesis has long been established. Our objective was to investigate COX-2 and NF-κB...Full Text Available

2010-01-01

476

Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV.

Activation of beta-adrenergic or muscarinic acetylcholine receptors expressed in transfected cells or epidermal growth factor receptors in human keratinocytes produces 15% to 200% changes in cellular...Full Text Available

1990-05-01

477

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

The cellular receptor for murine coronavirus mouse hepatitis virus (MHV)-A59 is a member of the carcinoembryonic antigen (CEA) family of glycoproteins in the immunoglobulin superfamily. We isolated...Full Text Available

1991-12-01

478

Cloning and expression of portions of the 34-kilodalton-protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease.

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

479

Chronic Cocaine-Induced H3 Acetylation and Transcriptional Activation of CaMKII? in the Nucleus Accumbens Is Critical for Motivation for Drug Reinforcement

Paratuberculosis (Johne's disease), an endemic mycobacteriosis of cattle that is caused by Mycobacterium paratuberculosis, is characterized by incoercible diarrhea and fecal shedding of bacteria. The...Full Text Available

1993-04-01

480

Characterization, phylogeny, alternative splicing and expression of Sox30 gene

The regulation of gene expression in the brain reward regions is known to contribute to the pathogenesis and persistence of drug addiction. Increasing evidence suggests that the regulation of gene transcription...Full Text Available

2010-03-01

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481

Characterization of the Decision Network for Wing Expansion in Drosophila Using Targeted Expression of the TRPM8 Channel

BackgroundMembers of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including...Full Text Available

482

Characterization of Terfestatin A, a New Specific Inhibitor for Auxin Signaling1[w

After emergence, adult flies and other insects select a suitable perch and expand their wings. Wing expansion is governed by the hormone bursicon and can be delayed under adverse environmental...Full Text Available

2009-03-18

483

Both common and specialty mushrooms inhibit adhesion molecule expression and in vitro binding of monocytes to human aortic endothelial cells in a pro-inflammatory environment

Terfestatin A (TrfA), terphenyl-β-glucoside, was isolated from Streptomyces sp. F40 in a forward screen for compounds that inhibit the expression of auxin-inducible...Full Text Available

2005-10-01

484

Biosynthesis and Uptake of Siderophores Is Controlled by the PacC-Mediated Ambient-pH Regulatory System in Aspergillus nidulans

BackgroundCardiovascular disease (CVD) is a leading cause of mortality in the United States as well as globally. Epidemiological studies show that regular fruit and vegetable consumption...Full Text Available

485

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

Biosynthesis and uptake of siderophores in Aspergillus nidulans are regulated not only by iron availability but also by ambient pH: expression of this high-affinity iron uptake system...Full Text Available

2004-04-01

486

Bacterial Artificial Chromosome Mutagenesis Using Recombineering

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

487

Astrocyte-Derived Vascular Endothelial Growth Factor Stabilizes Vessels in the Developing Retinal Vasculature

Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. BACs are large enough...Full Text Available

2011-01-01

488

Are Petals Sterile Stamens or Bracts? The Origin and Evolution of Petals in the Core Eudicots

Vascular endothelial growth factor (VEGF) plays a critical role in normal development as well as retinal vasculature disease. During retinal vascularization, VEGF is most strongly expressed by not yet...Full Text Available

489

Analysis of illegitimate genomic integration mediated by zinc-finger nucleases: implications for specificity of targeted gene correction

BackgroundThe aim of this paper is to discuss the controversial origins of petals from tepals or stamens and the links between the morphological expression of petals and floral organ...Full Text Available

2007-09-01

490

Algebraic analysis of the electromagnetic wave interaction with the two-level system with two-fold degenerated states

BackgroundFormation of site specific genomic double strand breaks (DSBs), induced by the expression of a pair of engineered zinc-finger nucleases (ZFNs), dramatically increases the...Full Text Available

491

Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein.

Algebraic properties of the analytical model, describing electro-magnetic weak interaction with the two-level system with two-fold degenerate state are considered. The expressions for the coherent states and Green function of the system are obtained.

1989-04-20

492

Agmatine attenuates brain edema through reducing the expression of aquaporin-1 after cerebral ischemia

To study the effect of agonist on the TRH (thyrotrophin-releasing hormone) receptor protein, an epitope-tagged receptor was stably expressed in HEK-293 cells (human embryonic kidney 293 cells) and receptor...Full Text Available

2004-06-15

493

Advantage and Choice: Social Relationships and Staff Assistance in Assisted Living

Brain edema is frequently shown after cerebral ischemia. It is an expansion of brain volume because of increasing water content in brain. It causes to increase mortality after stroke. Agmatine, formed...Full Text Available

2010-05-01

494

Advances in cancer tissue microarray technology: Towards improved understanding and diagnostics

Objectives.To understand how “cumulative inequality” (CI), expressed as individual advantage and choice, and “external social supports” contribute...Full Text Available

2010-05-01

495

A novel fluorescent pH probe for expression in plants

Over the past few years, tissue microarray (TMA) technology has been established as a standard method for assessing the expression of proteins or genes across large sets of tissue specimens....Full Text Available

2006-03-30

496

A gene expression signature shared by human mature oocytes and embryonic stem cells

BackgroundThe pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue...Full Text Available

497

A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides

BackgroundThe first week of human pre-embryo development is characterized by the induction of totipotency and then pluripotency. The understanding of this delicate process will have...Full Text Available

498

A Mouse Model Expressing a Truncated Form of Ameloblastin Exhibits Dental and Junctional Epithelium Defects

During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes...Full Text Available

2002-02-01

499

A Cellular J-Domain Protein Modulates Polyprotein Processing and Cytopathogenicity of a Pestivirus

SUMMARYAmeloblastin (AMBN) is the second most abundant extracellular matrix protein produced by the epithelial cells called ameloblasts and is found mainly in forming dental enamel....Full Text Available

2009-06-01

500