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Sample records for storage globulin glo-3a

  1. Lack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice.

    Science.gov (United States)

    Lee, Hye-Jung; Jo, Yeong-Min; Lee, Jong-Yeol; Lim, Sun-Hyung; Kim, Young-Mi

    2015-01-01

    The major seed storage proteins (SSPs) in rice seeds have been classified into three types, glutelins, prolamins, and globulin, and the proportion of each SSP varies. It has been shown in rice mutants that when either glutelins or prolamins are defective, the expression of another type of SSP is promoted to counterbalance the deficit. However, we observed reduced abundances of glutelins and prolamins in dry seeds of a globulin-deficient rice mutant (Glb-RNAi), which was generated with RNA interference (RNAi)-induced suppression of globulin expression. The expression of the prolamin and glutelin subfamily genes was reduced in the immature seeds of Glb-RNAi lines compared with those in wild type. A proteomic analysis of Glb-RNAi seeds showed that the reductions in glutelin and prolamin were conserved at the protein level. The decreased pattern in glutelin was also significant in the presence of a reductant, suggesting that the polymerization of the glutelin proteins via intramolecular disulfide bonds could be interrupted in Glb-RNAi seeds. We also observed aberrant and loosely packed structures in the storage organelles of Glb-RNAi seeds, which may be attributable to the reductions in SSPs. In this study, we evaluated the role of rice globulin in seed development, showing that a deficiency in globulin could comprehensively reduce the expression of other SSPs. PMID:26133242

  2. Lack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice

    Directory of Open Access Journals (Sweden)

    Hye-Jung Lee

    2015-06-01

    Full Text Available The major seed storage proteins (SSPs in rice seeds have been classified into three types, glutelins, prolamins, and globulin, and the proportion of each SSP varies. It has been shown in rice mutants that when either glutelins or prolamins are defective, the expression of another type of SSP is promoted to counterbalance the deficit. However, we observed reduced abundances of glutelins and prolamins in dry seeds of a globulin-deficient rice mutant (Glb-RNAi, which was generated with RNA interference (RNAi-induced suppression of globulin expression. The expression of the prolamin and glutelin subfamily genes was reduced in the immature seeds of Glb-RNAi lines compared with those in wild type. A proteomic analysis of Glb-RNAi seeds showed that the reductions in glutelin and prolamin were conserved at the protein level. The decreased pattern in glutelin was also significant in the presence of a reductant, suggesting that the polymerization of the glutelin proteins via intramolecular disulfide bonds could be interrupted in Glb-RNAi seeds. We also observed aberrant and loosely packed structures in the storage organelles of Glb-RNAi seeds, which may be attributable to the reductions in SSPs. In this study, we evaluated the role of rice globulin in seed development, showing that a deficiency in globulin could comprehensively reduce the expression of other SSPs.

  3. Proteolytic Cleavage at Twin Arginine Residues Affects Structural and Functional Transitions of Lupin Seed 11S Storage Globulin

    OpenAIRE

    Capraro, Jessica; Sessa, Fabio; Magni, Chiara; SCARAFONI, ALESSIO; Maffioli, Elisa; Tedeschi, Gabriella; Croy, Ron R. D.; Duranti, Marcello

    2015-01-01

    The 11S storage globulin of white lupin seeds binds to a metal affinity chromatography matrix. Two unusual stretches of contiguous histidine residues, reminiscent of the multiple histidines forming metal binding motifs, at the C-terminal end of 11S globulin acidic chains were hypothesized as candidate elements responsible for the binding capacity. To prove this, the protein was incubated with a lupin seed endopeptidase previously shown to cleave at twin arginine motifs, recurrent in the seque...

  4. Proteolytic cleavage at twin arginine residues affects structural and functional transitions of lupin seed 11S storage globulin.

    Science.gov (United States)

    Capraro, Jessica; Sessa, Fabio; Magni, Chiara; Scarafoni, Alessio; Maffioli, Elisa; Tedeschi, Gabriella; Croy, Ron R D; Duranti, Marcello

    2015-01-01

    The 11S storage globulin of white lupin seeds binds to a metal affinity chromatography matrix. Two unusual stretches of contiguous histidine residues, reminiscent of the multiple histidines forming metal binding motifs, at the C-terminal end of 11S globulin acidic chains were hypothesized as candidate elements responsible for the binding capacity. To prove this, the protein was incubated with a lupin seed endopeptidase previously shown to cleave at twin arginine motifs, recurrent in the sequence region of interest. Upon incubation with this enzyme, the loss of metal binding capacity paralleled that of the anti-his-tag reactive polypeptides. The recovered small proteolytic fragment was analyzed by mass spectrometry and N-terminal sequencing and found to correspond to the 24-mer region cleaved off at twin arginine residues and containing the natural his-tag-like region. Similarly, when lupin seeds were germinated for a few days, the his-tag containing 11S globulin chain was converted to a form devoid of such region, suggesting that this mechanism is a part of the natural degradatory process of the protein. The hypothesis that the ordered and controlled dismantling of storage proteins may generate peptide fragments with potential functional roles in plant ontogenesis is presented and discussed. PMID:25658355

  5. Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin

    OpenAIRE

    MacFarlane Amanda J; Melnyk Charles W; Loit Evelin; Scott Fraser W; Altosaar Illimar

    2009-01-01

    Abstract Background Exposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D). Recently, a wheat protein encoded by cDNA WP5212 has been shown to be antigenic in mice, rats and humans with autoimmune T1D. To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened. Results Three unique wheat globulin genes, Glo-3A...

  6. Serum globulin electrophoresis

    Science.gov (United States)

    Increased gamma globulin proteins may indicate: Acute infection Bone marrow cancer called multiple myeloma Chronic inflammatory disease (for example, rheumatoid arthritis and systemic lupus erythematosus ) Overactive immune system ( ...

  7. N-terminal amino acid sequences of chloroform/methanol-soluble proteins and albumins from endosperms of wheat, barley and related species: Homology with inhibitors of ?-amylase and trypsin and with 2 S storage globulins

    OpenAIRE

    Shewry, Peter; Lafiandra, Domenico; Salcedo Duran, Gabriel; Aragoncillo Ballesteros, Cipriano; García Olmedo, Francisco; Lew, Ellen; Dietler, Mary; Kasarda, Donald

    1984-01-01

    The N-terminal amino acid sequences of two chloroform/methanol soluble globulins from barley and one form wheat are reported. They are homologous with N-terminal sequences previously reported for ?-amylase and trypsin inhibitors from cereals and 2 S storage proteins from castor bean and rape. Three albumins were also purified from Aegilops squarrosa and Triticum monococcum. These had N-terminal amino acid sequences most closely related to the ?-amylase and trypsin inhibitors. The relationship...

  8. SHBG (Sex Hormone Binding Globulin)

    Science.gov (United States)

    ... as: Testosterone-estrogen Binding Globulin; TeBG Formal name: Sex Hormone Binding Globulin Related tests: Testosterone , Free Testosterone, ... I should know? How is it used? The sex hormone binding globulin (SHBG) test may be used ...

  9. The molecular biology and biochemistry of rice endosperm ?-globulin

    International Nuclear Information System (INIS)

    The author's first objective was to isolate a cDNA clone that encodes the rice endosperm ?-globulin. Purified antibodies against a rice storage protein, ?-globulin, were used to screen a ?gt11 cDNA expression library constructed from immature rice seed endosperm. The cDNA insert of clone 4A1 (identified by antibody screening) was used as a probe to identify long cDNA inserts in the library. The deduced amino acid sequence of clone A3-12 cDNA insert (identified by cDNA screening) contained the amino acid sequences of three cyanogen bromide peptides fragment of ?-globulin. The calculated molecular weight and amino acid composition of the deduced amino acid sequence were similar to the ?-globulin protein. Northern blot analysis indicated that mRNA of one size, approximately 1.0 kb, is expressed. Southern genomic blot analysis revealed one band with EcoRI or Hind III digestion. Cell-free translation and immunoprecipitation showed that the initial translation product is approximately 2,000 daltons larger than the mature protein. The amino acid sequence of ?-globulin revealed limited regions of similarities with wheat storage proteins. The author concludes that the cDNA insert in clone A3-12 contained the entire coding region of ?-globulin protein and that ?-globulin is encoded by a single gene. My second objective was to inhibit the degradation of ?-globulin in the salt extract of rice flour. The salt extract of rice flour contained an acid protease whose optimal pH was 3 for 3H-casein hydrolysis. A polypeptide with molecular weight of 20,000 was immunologically reactive with ?-globulin antibodies and is produced by limited proteolysis in the extract. Pepstatin inhibited the proteolysis of 3H-casein and slowed the proteolysis of ?-globulin

  10. Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae

    OpenAIRE

    M.P. Sales; P.P. Pimenta; N.S. Paes; Grossi-de-Sá, M. F.; Xavier-Filho, J.

    2001-01-01

    The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata) seed vicilins (7S storage proteins) bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vic...

  11. Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae

    Scientific Electronic Library Online (English)

    M.P., Sales; P.P., Pimenta; N.S., Paes; M.F., Grossi-de-Sá; J., Xavier-Filho.

    2001-01-01

    Full Text Available The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata) seed vicilins (7S storage proteins) bound to these structures suggested that C. maculatus-susceptible vicil [...] ins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae.

  12. Vicilins (7S storage globulins of cowpea (Vigna unguiculata seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae larvae

    Directory of Open Access Journals (Sweden)

    M.P. Sales

    2001-01-01

    Full Text Available The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata seed vicilins (7S storage proteins bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae.

  13. Deterministic pressure-induced dissociation of vicilin, the 7S storage globulin from pea seeds: effects of pH and cosolvents on oligomer stability.

    Science.gov (United States)

    Pedrosa, C; Ferreira, S T

    1994-04-01

    A thermodynamic characterization of subunit association of vicilin, a storage protein from pea seeds, was performed using a combination of hydrostatic pressure and fluorescence spectroscopy. Application of pressure up to 2.4 kbar caused dissociation of vicilin subunits, as revealed by (1) size-exclusion PPLC of pressurized samples, (2) fluorescence anisotropy measurements of a dansyl-vicilin conjugate under pressure, and (3) quenching of the intrinsic fluorescence of vicilin. Pressure dissociation data were well described by a model for dissociation of a trimer. This enabled calculation of the standard molar volume change of association (delta Vo) and the equilibrium dissociation constant at atmospheric pressure (Ko); at pH 10 these were found to be delta Vo = 146 mL/mol and Ko = 2.2 x 10(-15) M2, respectively, corresponding to C1/2 (the concentration of protein at 50% dissociation at atmospheric pressure) = 18 nM and a stabilization free energy of -19.6 kcal/mol for the oligomer. Vicilin exhibited an anomalously low dependence on protein concentration for pressure dissociation. This appeared related to conformational changes in the dissociated subunits, which caused a loss of ca. 5 kcal/mol in the free energy of association and to structural/energetic heterogeneity in the population of oligomers. Pressure dissociation was markedly pH-dependent, with a stabilization free energy loss of 3.4 kcal/mol upon raising pH from 9 to 10. Circular dichroism and intrinsic fluorescence lifetime measurements at atmospheric pressure showed that the structure of vicilin was largely unaffected by pH in the range investigated. These results suggest that the effect of pH may involve deprotonation of lysine residues participating in salt bridges between vicilin subunits. Pressure dissociation of vicilin was significantly inhibited by addition of salts (NaCl, KCl, LiCl) or glycerol. Dissociation curves obtained in the presence of salts enabled calculation of the free energies of stabilization (ranging from ca. -1.2 to -2.4 kcal/mol) of the vicilin oligomers by these cosolvents. The similar effects of salts or glycerol suggest a common mechanism of stabilization of the oligomer involving exclusion of the cosolvents from the protein interface and preferential hydration of the protein. PMID:8142407

  14. Purification of equine Gc-globulin

    DEFF Research Database (Denmark)

    Houen, Gunnar; Pihl, Tina Holberg

    Objectives With the aim of producing antibodies for an equine Group specific component (Gc)-globulin assay, the protein was purified from normal equine plasma. Methods Equine Gc-globulin was purified from healthy horse plasma using ion exchange chromatography (Q-Sepharose, CM-Sepharose) and preparative PAGE. Results Equine Gc-globulin has successfully been purified from healthy horse plasma and rabbits and mice are being immunized to produce specific antibodies. Conclusions Purification of equine Gc-globulin and the production of specific antibodies will make it possible to develop an assay for measuring Gc-globulin in horses. Studies in rodents and humans have shown that Gc-globulin is a multifunctional acute phase plasma protein, which removes actin from the blood by binding it and facilitating its clearance from the circulation by the liver. As such, Gc-globulin prevents hyper coagulation, shock and death in human patients with massive actin release caused by severe tissue injuries like physical trauma, sepsis, endotoxemia, or liver failure. Gc-globulin is consumed in this process, and the plasma concentration of free Gc-globulin hence decreases rapidly after tissue injury and has shown to be a sensitive marker of acute tissue injury and fatal outcome in humans. Patients with a low plasma concentration of Gc-globulin due to severe tissue injury might potentially benefit from infusions with purified Gc-globulin [1]. With an equine Gc-globulin assay, future studies will investigate the concentration of Gc-globulin in colic horses with intestinal ischemia were Gc-globulin might be useful as a diagnostic and prognostic marker. Horses with intestinal ischemia often die, despite of expensive surgical treatment, because of endotoxemia and shock, therefore these horses potentially could benefit from Gc-globulin infusions.

  15. Purification of equine Gc-globulin

    DEFF Research Database (Denmark)

    Houen, Gunnar; Pihl, Tina Holberg; Andersen, Pia Haubro; Jacobsen, Stine; Olsen, Dorthe Tange

    Objectives With the aim of producing antibodies for an equine Group specific component (Gc)-globulin assay, the protein was purified from normal equine plasma. Methods Equine Gc-globulin was purified from healthy horse plasma using ion exchange chromatography (Q-Sepharose, CM-Sepharose) and...... preparative PAGE. Results Equine Gc-globulin has successfully been purified from healthy horse plasma and rabbits and mice are being immunized to produce specific antibodies. Conclusions Purification of equine Gc-globulin and the production of specific antibodies will make it possible to develop an assay for...... to be a sensitive marker of acute tissue injury and fatal outcome in humans. Patients with a low plasma concentration of Gc-globulin due to severe tissue injury might potentially benefit from infusions with purified Gc-globulin [1]. With an equine Gc-globulin assay, future studies will investigate...

  16. Purification of equine Gc-globulin

    OpenAIRE

    HOUEN, GUNNAR; Pihl, Tina Holberg; Andersen, Pia Haubro; Jacobsen, Stine; Olsen, Dorthe Tange

    2010-01-01

    Objectives With the aim of producing antibodies for an equine Group specific component (Gc)-globulin assay, the protein was purified from normal equine plasma. Methods Equine Gc-globulin was purified from healthy horse plasma using ion exchange chromatography (Q-Sepharose, CM-Sepharose) and preparative PAGE. Results Equine Gc-globulin has successfully been purified from healthy horse plasma and rabbits and mice are being immunized to produce specific antibodies. Conclusions Purification of eq...

  17. Sex hormone binding globulin phenotypes

    DEFF Research Database (Denmark)

    Cornelisse, M M; Bennett, Patrick; Christiansen, M; Blaakaer, J; Gluud, C; Andersen, J R; Homann, C; Garred, P

    1994-01-01

    Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection....... This method of detection was used to determine the distribution of SHBG phenotypes in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and...... experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution....

  18. Genetics Home Reference: Corticosteroid-binding globulin deficiency

    Science.gov (United States)

    ... binding globulin deficiency and may include treatment providers. American Heart Association: Understanding Blood Pressure Readings Genetic Testing Registry: Corticosteroid-binding globulin deficiency You might ...

  19. Gc globulin as a diagnostic and prognostic marker in horses

    DEFF Research Database (Denmark)

    Pihl, Tina Holberg

    injury can prevent development of shock and thereby increase survival chances. The in vivo toxicity of Gc-globulin infusion is currently being investigated in horses and other species. Gc-globulin has been demonstrated in horse plasma and its structure closely resembles that of human Gc-globulin. Gc......-globulin concentrations in horses under clinical conditions have never previously been investigated. The Ph.D. project focuses on Gc-globulin as a prognostic marker in horses with acute abdominal pain....

  20. Profile of total protein, albumin, globulin and albumin globulin ratio in bulls

    Directory of Open Access Journals (Sweden)

    Ida Zahidah Irfan

    2014-06-01

    Full Text Available Determination of serum total protein concentration and main fractions (albumin and globulin can be used as an important diagnostic tool in clinical biochemistry. Several factors can affect the concentration of total protein, albumin, globulin and albumin globulin ratio (A/G. The aim of this study is to obtain serum protein profiles, albumin, globulin and A/G ratio based on breed, age and BCS (body condition score. Blood samples from 160 bulls were collected. Blood chemistry were analyzed by photometer principle using a commercial kit. There were significant (P<0.001 breed variation on total protein, albumin, globulin and albumin globulin ratio. Significant age differences were observed on total protein and albumin concentration (P<0.001, while globulin concentration and A/G ratio were also significant (P<0.05. Amongs groups of BCS, significant difference was verified only in the albumin concentration (P<0.05. The concentration of total proteins, albumins and globulins in the serum of the bulls are higher than standard values for cattle, while A/G ratio is lower.

  1. Total Protein and Albumin/Globulin Ratio Test

    Science.gov (United States)

    ... limited. Home Visit Global Sites Search Help? Total Protein and A/G Ratio Share this page: Was ... as: TP; Albumin/Globulin Ratio Formal name: Total Protein; Albumin to Globulin Ratio Related tests: Albumin ; Liver ...

  2. Antithymocyte globulin stimulates human hematopoietic progenitor cells.

    OpenAIRE

    Huang, A T; Mold, N G; Zhang, S F

    1987-01-01

    Antithymocyte globulin (ATG), a horse antihuman thymus antiserum highly effective in the majority of patients with aplastic anemia, was studied for its in vitro effects on hematopoietic progenitor cells. Marrow cells isolated by an immunoadherence technique with the HPCA-1 (human progenitor cell antigen) monoclonal antibody after removal of contaminating T cells and macrophages formed erythroid colonies in methyl cellulose media in the presence of ATG at concentrations of 25-50 micrograms/ml....

  3. 21 CFR 862.1330 - Globulin test system.

    Science.gov (United States)

    2010-04-01

    ...) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems § 862... other disorders of blood globulins. (b) Classification. Class I (general controls). The device is...

  4. Immune Reconstitution Following Rabbit Antithymocyte Globulin

    OpenAIRE

    Gurkan, S; Luan, Y.; Dhillon, N; Allam, S. R.; Montague, T; Bromberg, J.S.; Ames, S.; Lerner, S; Ebcioglu, Z.; Nair, V.; Dinavahi, R.; Sehgal, V; Heeger, P.; Schroppel, B.; Murphy, B

    2010-01-01

    Depletional induction therapies are routinely used to prevent acute rejection and improve transplant outcome. The effects of depleting agents on T-cell subsets and subsequent T-cell reconstitution are incompletely defined. We used flow cytometry to examine the effects of rabbit antithymocyte globulin (rATG) on the peripheral T-cell repertoire of pediatric and adult renal transplant recipients. We found that while rATG effectively depleted CD45RA+CD27+ naï?ve and CD45RO+CD27+ central memory CD...

  5. Metabolism of gamma-globulin in agammaglobulinaemia and hypogammaglobulinaemia

    International Nuclear Information System (INIS)

    In agammaglobulinaemia and analbuminaemia, both are diseases which cannot be explained by an abnormal protein loss, the low degradation rate is a forced consequence of the low rate of synthesis. The research in this paper gives no answer as to the real degradation capacity, because the limiting daily gamma-globulin which would lead to accumulation of gamma-globulin in an organism is not known. However, the experiments show clearly that the degradation capacity for gamma-globulin by agammaglobulinaemic subjects must be high. 2 tabs

  6. Dexamethasone suppresses sex-hormone binding globulin.

    Science.gov (United States)

    Blake, R E; Rajguru, S; Nolan, G H; Ahluwalia, B S

    1988-01-01

    Dexamethasone suppression (DEX-S) for 14 days has been used to determine the probable source of androgen excess. The exact mechanism(s) of DEX-S is still unclear. The authors postulated that dexamethasone (DEX) inhibits either the synthesis or secretion of sex-hormone binding globulin (SHBG). To examine this hypothesis, 14 women with polycystic ovarian disease (PCOD) and 3 volunteers were given DEX for 14 days. The PCOD group included obese and nonobese women (+/- 15% ideal body weight). Plasma determinations by radioimmunoassay of total testosterone, free testosterone, androstenedione, dehydroepiandrosterone sulfate, luteinizing hormone; follicle-stimulating hormone; cortisol, and SHBG were made. DEX suppressed SHBG levels (P less than 0.01). SHBG levels were significantly lower in the obese than in the nonobese (P less than 0.01). All androgens were suppressed by DEX, with the exception of androstenedione post-DEX levels, which were significantly greater than pre-DEX levels in 6 of 14 subjects (P greater than 0.05). This observation is consistent with DEX suppression of SHBG. PMID:3121398

  7. Mobilization of albumins and globulins during germination of Indian bean (Dolichos lablab L. var. lignosus) seeds

    OpenAIRE

    Ramakrishna, Vadde

    2007-01-01

    Albumin and globulin fractions of Indian bean seeds exhibit different mobilization patterns during the 8-day germination period. The albumin degradation was lesser in the early stage and reached a maximum in the later stages of germination. However, the globulins were degraded steadily from the start of imbibition. The influence of the embryonic axis on albumin and globulin degradation was also evaluated by using axis-attached and axis-detached cotyledons. The overall loss of globulins and...

  8. Treatment of neonatal sepsis with intravenous immune globulin

    DEFF Research Database (Denmark)

    Brocklehurst, Peter; Farrell, Barbara; King, Andrew; Juszczak, Edmund; Darlow, Brian; Haque, Khalid; Salt, Alison; Stenson, Ben; Tarnow-Mordi, William; Greisen, Gorm; Reinholdt, Jes

    2011-01-01

    Neonatal sepsis is a major cause of death and complications despite antibiotic treatment. Effective adjunctive treatments are needed. Newborn infants are relatively deficient in endogenous immunoglobulin. Meta-analyses of trials of intravenous immune globulin for suspected or proven neonatal sepsis...

  9. Treatment of neonatal sepsis with intravenous immune globulin

    DEFF Research Database (Denmark)

    Brocklehurst, Peter; Farrell, Barbara; King, Andrew; Juszczak, Edmund; Darlow, Brian; Haque, Khalid; Salt, Alison; Stenson, Ben; Tarnow-Mordi, William; Greisen, Gorm; Reinholdt, Jes

    2011-01-01

    Neonatal sepsis is a major cause of death and complications despite antibiotic treatment. Effective adjunctive treatments are needed. Newborn infants are relatively deficient in endogenous immunoglobulin. Meta-analyses of trials of intravenous immune globulin for suspected or proven neonatal sepsis suggest a reduced rate of death from any cause, but the trials have been small and have varied in quality.

  10. Immune Globulin Subcutaneous (Human) 20%: In Primary Immunodeficiency Disorders

    OpenAIRE

    McCormack, Paul L.

    2012-01-01

    Immune globulin subcutaneous 20% is a new high-concentration (200 g/L) solution of highly purified human IgG (?98%) indicated in the EU and the US for antibody replacement therapy in patients with primary immunodeficiency with antibody deficiency, and in the EU for replacement therapy in humoral immunodeficiency secondary to myeloma or chronic lymphocytic leukaemia.

  11. The treatment of connective tissue diseases with antilymphocyte globulin.

    Science.gov (United States)

    Denman, A M; Pelton, B K; Kinsley, M; Knight, S; Partridge, C; Walker, P; Bannister, B; Gumpel, M; Smith, D S; Loewi, E

    1976-01-01

    Immunosupprissive therapy was carried out using steroids, azathiprine and antilymphocyte globulin in one patient with systemic lupus erythematosus (SLE) and four with dermatomyositis. Response to treatment was based upon clinical evaluation. The patients with SLE and two of the four with dermatomyositis responded favourably to the regime. It is suggested that this preliminary study is sufficiently promising to warrant the creation of a rigidly controlled investigation. PMID:995782

  12. Polyclonal Antithymocyte Globulin and Cardiovascular Disease in Kidney Transplant Recipients

    OpenAIRE

    Ducloux, Didier; Courivaud, Cécile; Bamoulid, Jamal; Crepin, Thomas; Chalopin, Jean-Marc; Tiberghien, Pierre; Saas, Philippe

    2014-01-01

    T-lymphocyte activation may contribute to atherosclerosis, the prevalence of which is increased in transplant patients. However, the cardiovascular consequences of polyclonal antithymocyte globulin (ATG)–induced immune modifications, which include alterations in T-cell subsets, are unknown. We conducted a retrospective single-center study to assess whether ATG associates with an increased incidence of atherosclerotic events (CVEs) in kidney transplant patients. Propensity score analysis was p...

  13. Functionality of succinylated Brazil nut (Bertholletia excelsa HBK) kernel globulin.

    Science.gov (United States)

    Pinto Ramos, Cíntia Maria; Bora, Pushkar Singh

    2005-03-01

    One of the possible ways to improve the utilisation of defatted Brazil nut kernel flour, a by-product of oil extraction industries, is to improve its functional properties by chemical modification as it possesses very modest functional characteristics. Succinylated Brazil nut kernel globulin at 55.8%, 62.4% and 72.0% level showed a positive effect on functionality. The solubility of acylated globulin was improved above pH 4.0 but was reduced in the pH range of 3.0-4.0. Water absorption (1.96-4.00, 4.12, and 4.21 ml/g protein), oil absorption capacity (1.44-2.72, 2.80 and 2.94 ml/g protein) and apparent viscosity of the succinylated globulin increased with increase in the level of succinylation. The extent of modification also influenced emulsifying capacity, which showed a decrease at pH 3.0, but was increased at pH 5.0,7.0 and 9.0. Highest emulsion activity (approximately 63.0%) was observed at pH 3.0, followed by pH 9.0 and pH 7.0 and, least (about 11.8%) at pH 5.0. Emulsion stability also followed similar behaviour as that of emulsion activity. The improved functional properties of succinylated Brazil nut kernel globulin could be explored in a variety of food formulations such as high protein drinks, soups, bakery and meat products as well as in salad dressings and mayonnaise as an emulsifier. PMID:15898352

  14. Simplified method for measuring sex-hormone binding globulin

    International Nuclear Information System (INIS)

    We describe a simple, rapid method for measurement of sex-hormone binding globulin. Serial dilutions of pregnancy serum are prepared in serum from males that has been pre-treated by heating to 60 degrees C for 1 h to destroy endogenous binding globulin, which is then determined by a long-used technique to yield a set of ''standards.'' In the assay itself, a fixed amount of [3H]-labeled and unlabeled dihydrotestosterone is incubated with standard or unknown, and the bound fraction precipitated with saturated ammonium sulfate. A plot of percent of the steroid bound vs standard dilution yields a sigmoid curve, from which the results in unknowns can be read by simple extrapolation. Within-assay CVs for pools of serum from men, women, and women in late pregnancy were 6.56, 9.59, and 8.4%, respectively. Between-assay CVs for the same pools were 8.05, 9.5, and 11.5%, respectively. The correlation between results obtained by this method and those of the older technique was 0.95 for samples from non-pregnant subjects and 0.73 for those from pregnant women. Our procedure is simpler and faster than previous methods and accurately measures the differences in the globulin in sera from men, women, and pregnant women. Forty to 50 samples can be assayed in a working day

  15. The effect of 60Co gamma radiation on human serum gamma-globulin

    International Nuclear Information System (INIS)

    The effect of 60Co gamma radiation on human serum gamma-globulin was studied in vitro experiments. Solutions of 0.5 percent gamma-globulin were exposed to 0.2, 0.5, 1.0 and 1.9 Mrad doses 60Co gamma irradiation. Experiments showed that electrophoretic mobility of serum gamma-globulin decreased after gamma irradiation. No significant change in gamma-globulin UV absorption spectrum was observed at 0.2, 0.5, 1.0 and 1.9 Mrad doses. Gamma-globulin becomes progressively less soluble in water as the radiation doses is increased. Radiation induced transformation into insoluble gamma-globulin agregates and scission products. (author)

  16. Features of gamma-globulin denaturation at thermal and ?-radiating influences

    International Nuclear Information System (INIS)

    Effect of different doses of gamma radiation of 57Co isotope on the peculiarities in spectral alterations of globulin-gamma is studied as well as effect of 55 deg C and 70 deg C temperatures. Data on the specific character of hypochromic and hyperchromic effects, bathochromic and hypsochromic shifts in separate derivatives of the absorption spectrum of globulin-gamma are presented. Proposition on the exclusiveness of hyperchromic effect in case of globulin-gamma and albumin denaturation is removed

  17. Production of late IFN-? induced by plasma ?-globulin fraction proteins and their metal complexes.

    Science.gov (United States)

    Cheknev, S B; Babajanz, A A; Efremova, I E; Piskovskaya, L S

    2011-04-01

    Plasma ?-globulin fraction proteins, copper and zinc cations, and metal complexes they form with human serum ?-globulin induce the production of IFN-? by human blood cells throughout the periods of up to 72 h. Zinc cation-modified protein by 1.6 times (p<0.05) more actively induces late IFN-? than the control ?-globulin; ?-globulin-copper metal complex is 2-fold (p<0.002) more effective than the control protein. The results indicate that functional relationships between the components inducing the production of late IFN-? differ from the effects realized during the early period of induction. PMID:22235427

  18. Induction of TNF-? production by metal complexes of ?-globulin fraction proteins and copper and zinc cations.

    Science.gov (United States)

    Cheknev, S B; Efremova, I E; Apresova, M A; Babajantz, A A

    2013-04-01

    Plasma ?-globulin fraction proteins, copper and zinc cations, and metal complexes of these cations and human serum ?-globulin induce the production of TNF-? by human blood cells. The protein modified by zinc cations is by 1.4-1.7 times more potent (p<0.001-0.01) than control ?-globulin in inducing the production of TNF-?, while metal complex formed by ?-globulin and copper is by 1.9-2.2 times more potent that the control protein (p<0.001). Under conditions of experimental induction, TNF-? is produced as a typical early response cytokine. During long-term incubation, copper cations lose the ability to induce TNF-? production, while in combination with ?-globulin these cations produce a synergistic effect with the control protein. PMID:23658917

  19. Production of early IL-1? induced by human serum ?-globulin metal complexes.

    Science.gov (United States)

    Cheknev, S B; Efremova, I E; Piskovskaya, L S; Yushkovets, E N; Babajanz, A A

    2013-01-01

    Plasma ?-globulin fraction proteins, copper and zinc cations, and metal complexes formed by them with human serum ?-globulin induce the production of early (24-h incubation) IL-1? by human blood cells. The protein modified by Zn cations 1.2 times more actively (p<0.01) induced early IL-1? than the control ?-globulin, while ?-globulin metal complex with copper was 1.4 times less active (p<0.1) than the control protein. The regularities of induction changed over the course of 48-h incubation: zinc cations chelated by ?-globulin fraction protein reduced, while copper cations stimulated the realization of the protein induction potential in the metal complex. PMID:23484196

  20. Comparison of horse and rabbit anti-thymocyte globulin in immunosuppressive therapy for refractory cytopenia of childhood

    DEFF Research Database (Denmark)

    Yoshimi, Ayami; van den Heuvel-Eibrink, Marry M; Baumann, Irith; Schwarz, Stephan; Simonitsch-Klupp, Ingrid; de Paepe, Pascale; Campr, Vit; Birk Kerndrup, Gitte; O' Sullivan, Maureen; Devito, Rita; Leguit, Roos; Hernandez, Miguel; Dworzak, Michael; de Moerloose, Barbara; Stary, Jan; Hasle, Henrik; Smith, Owen P; Zecca, Marco; Catala, Albert; Schmugge, Markus; Locatelli, Franco; Führer, Monika; Fischer, Alexandra; Guderle, Anne; Nöllke, Peter; Strahm, Brigitte; Niemeyer, Charlotte M

    2014-01-01

    Refractory cytopenia of childhood is the most common subtype of myelodysplastic syndrome in children. In this paper, we compared the outcome of immunosuppressive therapy using horse anti-thymocyte globulin (n = 46) with that using rabbit anti-thymocyte globulin (n = 49) in 95 patients with refractory cytopenia of childhood and hypocellular bone marrow. The response rate at 6 months was 74% for horse anti-thymocyte globulin and 53% for rabbit anti-thymocyte globulin (p = 0.04). The inferior respo...

  1. Interactions of protein content and globulin subunit composition of soybean proteins in relation to tofu gel properties.

    Science.gov (United States)

    James, Andrew T; Yang, Aijun

    2016-03-01

    The content and globulin subunit composition of soybean proteins are known to affect tofu quality and food-grade soybeans usually have higher levels of proteins. We studied the tofu quality of soybeans with high (44.8%) or low (39.1%) protein content and with or without the 11S globulin polypeptide, 11SA4. Both protein content and 11SA4 significantly affected tofu gel properties. Soybeans containing more protein had smaller seeds which produced significantly firmer (0.663 vs.0.557 N, pgels with creamier colour. The absence of 11SA4 was positively correlated with seed size, tofu hardness and water holding capacity and led to significant changes to the profile of storage protein subunits, which may have contributed to the improvement in tofu gel properties. These results suggest that, in combination with higher protein content, certain protein subunits or their polypeptides can also be targeted in selecting soybeans to further improve soy food quality. PMID:26471556

  2. Identification and characterization of wheat grain albumin/globulin allergens.

    Science.gov (United States)

    Weiss, W; Huber, G; Engel, K H; Pethran, A; Dunn, M J; Gooley, A A; Görg, A

    1997-05-01

    Bakers' asthma, an immediate-type allergic response to the inhalation of cereal flours, is an important occupational disease among workers of the baking and milling industries, and the salt-soluble proteins of wheat and rye flour dust are considered the most relevant allergens. In order to identify and characterize the major IgE-binding proteins, the polypeptide composition of the albumin/globulin protein fraction obtained from different cultivars was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and high-resolution two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt), followed by immunoblotting with sera from asthmatic bakers. Relevant allergens were isolated by micropreparative IPG-Dalt and blotting onto polyvinylidenedifluoride membranes and identified by amino acid composition analysis or N-terminal amino acid sequence analysis. SDS-PAGE, IPG-Dalt, and immunoblotting demonstrated that the sera of the bakers allergic to flour contained IgE antibodies which bound to numerous albumin/globulin polypeptides in the 70, 55, 35, 26-28, and 14-18 kDa areas. More detailed investigations using IPG-Dalt revealed cultivar-specific differences in IgE-binding. It was also demonstrated that the majority of the allergens were not single polypeptide spots, but consisted of up to ten isoforms of similar molecular mass but different isoelectric points. Amino acid composition analysis and N-terminal amino acid sequence analysis, which were performed for nine allergens located in the 14-18, 26-28, and 35 kDa areas, revealed homologies to amylase/protease inhibitors, acyl-CoA oxidase and fructose-bisphosphate-aldolase from wheat, barley, maize, and rice, respectively. PMID:9194615

  3. Subunit structure and functional properties of the predominant globulin of perilla (Perilla frutescens var. frutescens) seeds.

    Science.gov (United States)

    Takenaka, Yasuyuki; Arii, Yasuhiro; Masui, Hironori

    2010-01-01

    Approximately 40% of defatted perilla seeds consists of proteins which are primarily composed of globulin (84%). The amino acid profile of perilla proteins demonstrated balanced amounts of all essential amino acids, except for lysine. The molecular mass of the predominant globulin was estimated to be 340 kDa by gel filtration. This globulin was separated into three intermediary subunits (54, 57 and 59 kDa) by SDS-PAGE. It is suggested from these results that the globulin exists as a hexamer. A treatment with 50 mM dithiothreitol enabled the intermediary subunits to be separated into three acidic subunits (31-34 kDa) and four basic subunits (23-25 kDa). It is interesting that this subunit structure is the same as that of sesame ?-globulin, despite them coming from different families. Compared to sesame ?-globulin, the heat-induced gel of perilla globulin had better water-holding ability, despite it displaying the same degree of gel hardness. PMID:21150107

  4. Studies on the role of glycosylation for human corticosteroid-binding globulin: Comparison with that for thyroxine-binding globulin

    International Nuclear Information System (INIS)

    The role of glycosylation on the secretion and the stability of human corticosteroid binding globulin (CBG) was studied. Cells of the human hepatoma line were labeled by [35S]methionine in presence of or absence of tunicamycin (TM). Media or cells were harvested at 0, 3, 6, and 20 h after the addition of excess unlabeled methionine. Media and cell lysates were incubated with anti-CBG serum and immune complexes were precipitated with Staphylococcus aureus protein A (Pansorbin). Immunoprecipitates were analyzed by fluorography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoprecipitation of T4-binding globulin (TBG) was also carried out with anti-TBG serum. Fluorographic analysis revealed three forms of CBG: CBG1, a glycosylated, mature, and secretory form with apparent mol wt of 70 K; CBG2, a glycosylated precursor which due to incomplete carbohydrate processing has an apparent mol wt of 54 K; and CBG3, a nonglycosylated form consisting of the 40 K core protein. In absence of TM, CBG1 was observed in media and CBG2 was detected in cell lysates. The proportion of CBG1 increased during the chase, whereas that of CBG2 decreased, indicating that CBG was secreted after processing of the oligosaccharides on CBG2. In presence of TM, CBG3 was found both in media and cell lysates. The sum of CBG3 in the medium and the cell lysate decreased during the chase, whereas that of CBG1 and CBG2 remained unchanged. Similar to CBG, TBG1 (mature form, 60 K) and TBG2 (partially processed glycosylated form, 54 K) were observed in media and cell lysates, respectively, in absence of TM. However, TBG3 (nonglycosylated, 44 K) was not detected in medium. These results indicate that glycosylation is not a key factor for the secretion of CBG but is important for its stability. On the other hand the glycosylation is indispensable for the secretion of TBG

  5. Labeling of human immune gamma globulin with sup(99m)Tc

    International Nuclear Information System (INIS)

    Human immune serum gamma globulin and rabbit anti-Stap. aureus antibody have been successfully labeled with sup(99m)Tc at pH 7.4 with an average binding efficiency of 86 and 82%, respectively. The labeled proteins behave similarly to unlabeled gamma-globulin fraction in the normal human serum as demonstrated by protein electrophoresis. The biological half-time of sup(99m)Tc-gamma-globulin in dog has been determined to be 54 min for the fast component and 14.7 hr for a slower component. Immunological assays demonstrate no significant change in antibody activity after labeling process. (author)

  6. CONFORMATION CHANGES OF HUMAN SERUM ?–GLOBULIN IN THE PRESENCE OF ZINC IONS

    Directory of Open Access Journals (Sweden)

    S. B. Cheknev

    2014-07-01

    Full Text Available Abstract. Conformational changes of human serum ?–globulin during interaction with the zinc ions were studied in a solution. It has been shown that the presence of zinc in over its physiological concentrations led to increase in optical density across the whole spectrum of ?–globulin ultraviolet absorption. On the contrary, hypochromia in the spectrum was registered after interaction of the protein with zinc used in subphisiological concentrations. Possible role of divalent metal cations in changes in conformation of the blood serum ?–globulins, and thereby in regulation of their effector functions was discussed. (Med. Immunol., 2005, vol.7, ? 4, pp. 375–380

  7. ?-Globulin fraction proteins and their metal complexes with copper cations in induction of IL-8 production.

    Science.gov (United States)

    Apresova, M A; Efremova, I E; Babayants, A A; Cheknev, S B

    2014-04-01

    Plasma ?-globulin fraction proteins, copper cations, and metal complexes formed by copper cations with human serum ?-globulin induce the production of up to 4.0 ng/ml IL-8 by human blood cells. Protein modified by copper cations is 1.3-1.7-fold (p < 0.001-0.01) more potent than control ?-globulin and 1.3-fold (p < 0.001) more potent than copper cations alone. Analysis of the time course of IL-8 production demonstrated that IL-8 is produced as a prolonged or delayed response cytokine under conditions of this induction. PMID:24824707

  8. INDUCTION OF IL-8 PRODUCTION BY THE METAL COMPLEXES OF ?-GLOBULIN FORMED WITH ZINC IONS

    Directory of Open Access Journals (Sweden)

    M. A. Apresova

    2014-07-01

    Full Text Available Abstract. This study has shown detectable production of IL-8, as a fraction of cytokine pool induced in culture of human peripheral blood cells (PBCs. Mean amounts of IL8 produced in presence of ?-globulin fraction proteins and/or their metal complexes with zinc ions varied from 575.0±27.77 to 6355.0±480.98 pg/ ml. The complexes of ?-globulin with zinc (48 h of culture caused increase of IL-8 production that was 1.8-fold higher than with control ?-globulin and 1.4-fold higher than with of zinc ions alone (p < 0.02. Dynamic tracking of the cytokine levels has shown that PBCs induced with ?-globulin/metal complexes produced IL8 as a factor of prolonged or late-type response.

  9. SIFAT FUNGSIONAL PRODUK INTERAKSI FRAKSI GLOBULIN 7S KOMAK (Dolichos lablab DAN GUM XANTAN [Functional Properties of the Interaction Product Between Globulin of 7S Fraction of Lablab Bean (Dolichos lablab with Xantan Gum

    Directory of Open Access Journals (Sweden)

    Sukamto1*

    2009-12-01

    Full Text Available Lablab bean (Dolichos lablab seeds is a potential source of protein globulin.The bean’s protein content is 20.86 %, and the amount of globulin was more than 60% from the total protein, having major fractions of 7S and 11S. The objectives of this research were to explore the 7S globulin fractions, to study interaction between 7S globulin fractions with xanthan gum, and to observe the functional properties of the product of the interaction. The research was conducted in 2 steps. The first step was to fractionate the 7S fractions from globulin. The second steps was to interact 7S globulin fraction with xanthan gum. The yield of these interaction were examined for its physicochemical and functional properties. The results showed that the 7S globulin fractions could be interacted by xanthan gum at pH 7. The interacted product of globulin 7S fraction 10 % with xanthan gum 0,75 % had good functional properties than globulin 7S fraction, such as oil holding capacity, foaming capacity, and emulsion activity. Water holding capacity could not be detected because the yield became soluble. However,the foaming and emulsifying stability were still lower than those of soybean protein isolates. The research concluded that xanthan gum could be used to improve the physicochemical and functional properties of globulin 7S fraction.

  10. Globulin-platelet model predicts minimal fibrosis and cirrhosis in chronic hepatitis B virus infected patients

    OpenAIRE

    Xu-Dong Liu; Jian-Lin Wu; Jian Liang; Tao Zhang; Qing-Shou Sheng

    2012-01-01

    AIM: To establish a simple model consisting of the routine laboratory variables to predict both minimal fibrosis and cirrhosis in chronic hepatitis B virus (HBV)-infected patients. METHODS: We retrospectively investigated 114 chronic HBV-infected patients who underwent liver biopsy in two different hospitals. Thirteen parameters were analyzed by step-wise regression analysis and correlation analysis. A new fibrosis index [globulin/platelet (GP) model] was developed, including globulin (GLOB) ...

  11. Gc globulin as a diagnostoc and prognostic marker in horses : A Ph.d. project

    OpenAIRE

    Pihl, Tina Holberg

    2008-01-01

    Group specific (Gc) globulin also known as vitamin D-binding protein is part of the extracellular actin-scavenging system that removes actin from the circulation. Actin is an intracellular structural protein, which is released to blood in patients with tissue injury and cell death. Circulating actin forms filaments, which cause microthrombi and endothelial injury. These effects of circulating actin are extremely harmful, and high levels of free actin are potentially lethal. Gc-globulin binds ...

  12. Thermal denaturation of sunflower globulins in low moisture conditions

    International Nuclear Information System (INIS)

    DSC analysis in pressure resisting pans of sunflower oil cake makes appear the endothermic peak of sunflower globulins denaturation. Its temperature decreases from 189.5 to 119.9 deg. C while the corresponding enthalpy increases from 2.6 to 3.3 J/g of sample, or from 6.7 to 12.2 J/g of dry protein, when the samples moisture content varies from 0 to 30.0% of the total weight. The plot of the denaturation temperature versus the moisture content is not linear but has a rounded global shape and seems to follow the hydration behavior of the proteins, modeled with the sorption isotherm. As it can be seen on scanning electron microscopy (SEM) micrographs, protein corpuscles 'melt' after such a thermal treatment and large aggregates form by coagulation. Moisture dependence of the 'fusion' temperature of native proteic organization, in low moisture conditions, offers so a new characterization method for the use of vegetable proteins in agro-materials

  13. Intravenous immune globulin suppresses angiogenesis in mice and humans

    Science.gov (United States)

    Yasuma, Reo; Cicatiello, Valeria; Mizutani, Takeshi; Tudisco, Laura; Kim, Younghee; Tarallo, Valeria; Bogdanovich, Sasha; Hirano, Yoshio; Kerur, Nagaraj; Li, Shengjian; Yasuma, Tetsuhiro; Fowler, Benjamin J; Wright, Charles B; Apicella, Ivana; Greco, Adelaide; Brunetti, Arturo; Ambati, Balamurali K; Helmers, Sevim Barbasso; Lundberg, Ingrid E; Viklicky, Ondrej; Leusen, Jeanette HW; Verbeek, J Sjef; Gelfand, Bradley D; Bastos-Carvalho, Ana; De Falco, Sandro; Ambati, Jayakrishna

    2016-01-01

    Human intravenous immune globulin (IVIg), a purified IgG fraction composed of ~ 60% IgG1 and obtained from the pooled plasma of thousands of donors, is clinically used for a wide range of diseases. The biological actions of IVIg are incompletely understood and have been attributed both to the polyclonal antibodies therein and also to their IgG (IgG) Fc regions. Recently, we demonstrated that multiple therapeutic human IgG1 antibodies suppress angiogenesis in a target-independent manner via FcγRI, a high-affinity receptor for IgG1. Here we show that IVIg possesses similar anti-angiogenic activity and inhibited blood vessel growth in five different mouse models of prevalent human diseases, namely, neovascular age-related macular degeneration, corneal neovascularization, colorectal cancer, fibrosarcoma and peripheral arterial ischemic disease. Angioinhibition was mediated by the Fc region of IVIg, required FcγRI and had similar potency in transgenic mice expressing human FcγRs. Finally, IVIg therapy administered to humans for the treatment of inflammatory or autoimmune diseases reduced kidney and muscle blood vessel densities. These data place IVIg, an agent approved by the US Food and Drug Administration, as a novel angioinhibitory drug in doses that are currently administered in the clinical setting. In addition, they raise the possibility of an unintended effect of IVIg on blood vessels. PMID:26925256

  14. Immunomodulatory treatment of myelodysplastic syndromes: antithymocyte globulin, cyclosporine, and alemtuzumab.

    Science.gov (United States)

    Parikh, Ankur R; Olnes, Matthew J; Barrett, A John

    2012-10-01

    It is now well accepted that a subgroup of patients with myelodysplastic syndromes (MDS) can recover from pancytopenia following immunosuppressive treatment (IST). For many years immunosuppression with antilymphocyte antibodies has been a standard treatment approach for patients with severe aplastic anemia (SAA). The initial concept of using immunosuppression to treat pancytopenic patients with MDS was based on the premise that MDS might share with SAA an autoimmune basis for the bone marrow failure common to both conditions. The idea was supported by reports of favorable outcomes in occasional cases of MDS treated with antithymocyte globulin (ATG). Today, various forms of IST have been successfully used to restore hematopoiesis in MDS in many centers worldwide. In this review we outline the rationale for use of IST in MDS, and describe studies which help to define the patients with MDS likely to respond to IST. We summarize 18 published clinical trials using IST for MDS and discuss how these studies have helped to define the MDS subgroups likely to respond to treatment, the nature and durability of the response, the impact of IST on long-term outcome, and the best treatment approach. PMID:23079060

  15. Radioimmunoassay of human sex hormone binding globulin: improved radioiodination procedure

    International Nuclear Information System (INIS)

    Sex hormone binding globulin (SHBG), purified by affinity chromatography from retroplacental blood plasma, was reacted with 3-(p-hydroxyphenyl) propionic acid N-hydroxysuccinimidyl ester (PHPPS, Bolton-Hunter reagent). The derivative of SHBG obtained (parahydroxyphenylpropionyl-SHBG; PHPP-SHBG) was stable and could, in contrast to underived SHBG, be efficiently 125I-iodinated with a lactoperoxidase technique. The PHPP-SHBG labelled with 125I had good antiserum binding and stability properties and was used for radioimmunoassay (RIA) of SHBG in serum. The RIA requires a total incubation time of 3 h. It has been standardized with purified SHBG and has a sensitivity of 5 ?g/l, giving a lowest detectable concentration in the routine procedure of about 0.2 mg/l. Variation within and between assay was 4.1% and 7.2%, respectively, for samples with values within the normal range. Values obtained by this RIA procedure correlate well with those obtained by a dihydrotestosterone binding method and by an electroimmunoassay technique. The mean serum concentration of SHBG in healthy, regularly menstruating women (n=42) was 3.7+-1.0 mg/l and in healthy men (n=100) 2.0+-0.9 mg/l. (Author)

  16. Polyclonal antithymocyte globulin and cardiovascular disease in kidney transplant recipients.

    Science.gov (United States)

    Ducloux, Didier; Courivaud, Cécile; Bamoulid, Jamal; Crepin, Thomas; Chalopin, Jean-Marc; Tiberghien, Pierre; Saas, Philippe

    2014-06-01

    T-lymphocyte activation may contribute to atherosclerosis, the prevalence of which is increased in transplant patients. However, the cardiovascular consequences of polyclonal antithymocyte globulin (ATG)-induced immune modifications, which include alterations in T-cell subsets, are unknown. We conducted a retrospective single-center study to assess whether ATG associates with an increased incidence of atherosclerotic events (CVEs) in kidney transplant patients. Propensity score analysis was performed to address potential confounding by indication. We also tested whether ATG use induces a proatherogenic immune status. Sixty-nine (12.2%) CVEs occurred during follow-up (87±31 months). The cumulative incidence of CVEs was higher in ATG-treated patients (14.7% versus 8.2%; P=0.03). Cox regression analysis revealed that ATG use was an independent risk factor for CVEs (hazard ratio [HR], 2.36; 95% confidence interval [95% CI], 1.35 to 4.13; P=0.003). Results obtained in the propensity score match analysis recapitulated those obtained from the overall cohort (HR, 2.09; 95% CI, 1.11 to 3.98; P=0.02). Late-stage differentiated CD8(+) T cells increased 1 year after transplantation only in ATG-treated patients. More generally, ATG associated with features of immune activation. These modifications increased markedly in patients exposed to cytomegalovirus (CMV). Subanalyses suggest that the effect of ATG on CVEs is restricted to CMV-exposed patients. However, CMV infection associated significantly with CVEs only in ATG-treated patients (HR, 2.07; 95% CI, 1.16 to 3.70; P=0.01). In conclusion, ATG associated with both immune activation and post-transplant CVEs in this cohort. Further studies should precisely determine whether ATG-induced immune activation is the causal link between ATG and CVEs. PMID:24511120

  17. Interactions between carbon nanodots with human serum albumin and ?-globulins: The effects on the transportation function.

    Science.gov (United States)

    Xu, Zi-Qiang; Yang, Qi-Qi; Lan, Jia-Yi; Zhang, Jia-Qi; Peng, Wu; Jin, Jian-Cheng; Jiang, Feng-Lei; Liu, Yi

    2016-01-15

    Carbon nanodots (C-dots) have attracted great attention as a new class of luminescent nanomaterials due to their superior physical and chemical properties. In order to better understand the basic behavior of C-dots in biological systems, a series of photophysical measurements were applied to study the interactions of C-dots with human serum albumin (HSA) and ?-globulins. The fluorescence of proteins was quenched by the dynamic mechanism rather than the formation of a protein/C-dots complex. The apparent dissociation constants of the C-dots bound to HSA and ?-globulins were of the same order of magnitude. Furthermore, it is proven that C-dots showed little influence on the conformation of HSA and ?-globulins. In addition, Fourier transform infrared and fluorescence spectroscopic studies demonstrated that the interaction between C-dots and two kinds of serum proteins was driven by hydrophobic and van der waals forces. Since the bioavailability of drugs can be modulated by their interactions with proteins, the variations of binding constants of three drugs with HSA and ?-globulins in the presence of different concentrations of C-dots (0-84?molL(-1)) have also been analyzed in this work, to reflect the effect of C-dots on the transportation function of HSA and ?-globulins. PMID:26368798

  18. PRODUCTION OF IL-18 IN PRESENCE OF METAL-?-GLOBULIN COMPLEXES

    Directory of Open Access Journals (Sweden)

    S. B. Cheknev

    2014-07-01

    Full Text Available Present  study  used  in  vitro  cultures  of  human  peripheral  blood  cells  (PBC with  addition  of ?-globulins, or  their complexes with copper and zinc, or  single copper and zinc  ions. We have  shown  that, among  common  pool  of  induced  cytokines,  interleukin-18  (IL-18  was detectable  at  a concentration  of 24.4±1.03 to 33.25±0.90 pg/ml. IL-18 production in PBC after 24h treatment with ?-globulin-zinc complexes zinc was higher by 13-19%  than  following 24-h  incubation with control proteins, or zinc  ions. Accordingly,   IL-18 production in PBC after 72-h  incubation with ?-globulin copper  complexes  was higher than with control proteins.  In parallel  to  IL-18, human  PBC  produce detectable   interferon  (IFN, up  to  32 U/ml.  We have  shown  a  strong positive correlation between  the peaks of IFN  increase,  and elevated IL-18  levels  caused  by ?-globulin-metal  complexes.  Hence,  an opportunity  for  regulation of intracellular IL-18  and  IFN  production  by  ?-globulin-metal  complexes  at  the  level  of  basal  cell  functions  was  demonstrated  in  context  of  IFN? induction, thus causing Th1-directed shift of immune response.

  19. EVALUATION OF IL-6 PRODUCTION BY HUMAN BLOOD CELLS INCUBATED WITH METAL COMPLEXES OF ?-GLOBULIN

    Directory of Open Access Journals (Sweden)

    S. B. Cheknev

    2014-07-01

    Full Text Available Abstract. This study has shown that that a common cytokine pool induced in cultured human peripheral blood cells (PBC supplied by either ?-globulin fraction proteins, copper or zinc cations, or appropriate metal complexes, contains detectable amounts of IL-6 (0.39+0.14 to 2.04+0.16 ng/ml. ?-globulin complexes with zinc or copper ions are able to induce production of IL-6 in amounts differing from those induced by control proteins, or copper and zinc ions used alone. IL-6 production by PBC in presence of ?-globulin/zinc complexes was 1.5 to 2.5-fold higher than with control proteins or single zinc ions at 24, 48 and 72 hrs of observation, whereas IL-6 production by PBC in presence of ?-globulin complexes with copper was higher than with appropriate control proteins or copper ions used alone for 48 and 72 hrs of cell incubation (resp., 1.7 to 2.4 and 2.8 to 4.6-fold increase. Possible role of copper and zinc ions chelated by ?-globulins from the microenvironment and modifying the Fc regions of antibodies, is considered a potential regulatory factor of IL-6 production by human PBC.

  20. Effect of Co-60 irradiation on hyperimmune antimeningococcus globulins-gamma

    International Nuclear Information System (INIS)

    Globulins-gamma from voluntary blood donors immunized with the Cuban BC antimeningococcus vaccine is now being used in our country for the treatment of the meningococcus disease. This study of the effect of Co-60 irradiation on antimeningococcus globulins-gamma was carried out to try to eliminate the inconvenience shown by the traditionally used sterilization procedures (losses in the filter and persistence of viral contamination). globulins-gamma was obtained by ethanol fractionation and was irradiated at a different dose in solution with different stabilizers and it was also lyophilized. Results of the chemical controls carried out lead to the conclusion that it is possible to use radiosterilization on this product in a lyophilized form. The preservation of bactericidal activity, even after the highest irradiation doses, confirms the above mentioned. 13 refs

  1. Interaction of serum sex steroid-binding globulin with cell membranes of human decidual tissue

    International Nuclear Information System (INIS)

    The interaction of the sex steroid-binding globulin (SBG) of human blood with plasma membranes of cells from human decidual tissue - the target tissue of estradiol - was studied. It was shown that SBG in complex with estradiol is capable of interacting specifically with these membranes. The dissociation (K/sub dis/) of this interaction is equal to (3.5 +/- 2.0) 10-12 M. The interaction of the SBG-estradiol complex with the membranes is characterized by high selectivity: such blood serum globulins as albumin, orosomucoid, transferrin, transcortin, and thyroxine-binding globulin do not compete with SBG for its binding sites on the membranes. The SBG-testosterone complex and SBG without steroid are also incapable of interacting with the membranes

  2. Detection of IFN-alpha produced in the presence of plasma gamma-globulin fraction proteins.

    Science.gov (United States)

    Cheknyov, S B; Babayants, A A; Efremova, I E; Yushkovets, E N

    2009-05-01

    Interferon-alpha was detected in IFN pool produced by human leukocytes in the presence of gamma-globulin fraction proteins, copper and zinc cations, and metal-modified gamma-globulins. The cytokine appeared in culture medium at early terms (24 h) of incubation, is characterized by acid resistance, and is neutralized by antibodies to IFN-alpha. The content of IFN-alpha in supernatants of induced leukocytes reached 60-90 pg/ml and correlated with antiviral activity of the samples. Zinc bound to human serum gamma-globulin attenuated and copper stimulated the realization of IFN-inducing characteristics of the protein at early terms of incubation. PMID:19907752

  3. ALTERATIONS IN TOTAL PROTEIN CONCENTRATION, SERUM PROTEIN FRACTIONS AND ALBUMIN/GLOBULIN RATIO IN HEALTHY RABBITS

    Directory of Open Access Journals (Sweden)

    Nuzhat Sultana

    2013-08-01

    Full Text Available This study assessed the effect of oral administration of Aloe vera and was to evaluate total serum protein, albumin and globulin concentrations as well as albumin / globulin (A / G ratio. Twenty rabbits weighing 1000 – 1800 g were divided into 2 groups. Each group consisted of ten animals. One served as control and other group served as experimental group. Results show that animals after 07, 15 and 30 days dosing of Aloe vera showed highly significant decrease in total protein and globulin and highly significant decrease in Albumin after 15 and 30 days of dosing of Aloe vera in comparison to control animals group. It is concluded that the long-term use of Aloe vera may cause hypoglobinemia and hypoalbuminemia at 30 days of dosing and it could be due to the liver diseases, evidence of hepatotoxicity induced Aloe vera also reported in previous studies.

  4. Comparison of horse and rabbit anti-thymocyte globulin in immunosuppressive therapy for refractory cytopenia of childhood

    DEFF Research Database (Denmark)

    Yoshimi, Ayami; van den Heuvel-Eibrink, Marry M

    2014-01-01

    Refractory cytopenia of childhood is the most common subtype of myelodysplastic syndrome in children. In this paper, we compared the outcome of immunosuppressive therapy using horse anti-thymocyte globulin (n = 46) with that using rabbit anti-thymocyte globulin (n = 49) in 95 patients with refractory cytopenia of childhood and hypocellular bone marrow. The response rate at 6 months was 74% for horse anti-thymocyte globulin and 53% for rabbit anti-thymocyte globulin (p = 0.04). The inferior response in the rabbit anti-thymocyte globulin group resulted in lower 4-year-transplantation-free (69% vs. 46%; p = 0.003) and failure-free (58% vs. 48%; p = 0.04) survival in this group compared with those in the horse anti-thymocyte globulin group. However, because of successful second-line hematopoietic stem cell transplantation, overall survival was comparable between groups (91% vs. 85%, p = ns). The cumulative incidence of relapse (15% vs. 9%, p = ns) and clonal evolution (12% vs. 4%, p = ns) at 4 years was comparable between groups. Our results suggest that the outcome of immunosuppressive therapy with rabbit anti-thymocyte globulin is inferior to that of horse anti-thymocyte globulin. Although immunosuppressive therapy is an effective therapy in selected patients with refractory cytopenia of childhood, the long-term risk of relapse or clonal evolution remains. (ClinicalTrial.gov identifiers: NCT00662090).

  5. Studies on a possible using of penicillin and specific globulin for treatment of Siberia ulcer infection in irradiated animals

    International Nuclear Information System (INIS)

    The efficiency of anti-anthracic globulin and penicillin for treating infectious anthrax was compared in experiments on 160 guinea pigs and 400 white mice irradiated with sub-lethal doses of cobalt-60 gamma rays. It was found that penicillin retained its effectiveness in the irradiated animals whereas anti-anthracic globulin lost much of its therapeutic efficiency. (auth.)

  6. [Variability of some blood serum beta-globulins in the life cycle of the true sturgeons (Acipenseridae)].

    Science.gov (United States)

    Subbotkin, M F; Subbotkina, T A

    2008-01-01

    Blood proteins of true sturgeons of three genera Acipenser, Huso, and Pseudocaphirhynchus preserving unidirectional variability in ontogenesis regardless of peculiarities of the fish ecology can indicate unity of origin of the current species. Age-related protein transformations have heterochronous character. The slowest ontogenetic development lasting 4-6 years is peculiar to one of antigens of beta-globulins. Analysis of variability of beta-globulins in the age diapason beginning from the 4-month-old fry and including adult spawned individuals indicates different functional significance of their individual components. The beta A-globulin absent at early postembryonic stages becomes the second, after albumin, quantitatively predominant antigen in the adult fish blood. The amount of beta B-globulin, like antigens of alpha 1- and alpha 2-globulins preserving relative ontogenetic stability, can decrease significantly in freshwater sturgeons due to their preparation for spawning. PMID:18767555

  7. 78 FR 79469 - Strategies To Address Hemolytic Complications of Immune Globulin Infusions; Public Workshop

    Science.gov (United States)

    2013-12-30

    ... HUMAN SERVICES Food and Drug Administration Strategies To Address Hemolytic Complications of Immune... Address Hemolytic Complications of Immune Globulin Infusions.'' The purpose of the public workshop is to..., firm name, address, telephone, and fax numbers) to Chris Nguyen (see Contact Person) or email...

  8. Is the sex hormone binding globulin related to preeclampsia independent of insulin resistance

    International Nuclear Information System (INIS)

    Objective: To evaluate the association between Sex Hormone Binding Globulin and preeclampsia in Iranian women considering the probable confounding effect of insulin resistance. Methods: The case-control study was conducted at the Semnan University of Medical Sciences, Iran, and comprised pregnant women who received prenatal care at Amiralmomenin Hospital in 2011. Cases represented patients admitted because of preeclampsia, while controls were randomly selected eligible pregnant women without hypertension and/or proteinuria. Fasting blood sugar and insulin were assessed for all participants as well as their blood concentration of Sex Hormone Binding Globulin. The Homeostasis Model Assessment of Insulin Resistance Score was used. The correlation between dependant and independent variables was reported by crude and adjusted odds ratio applying logistic regression models. SPSS 16.0 was used for statistical analysis. Results: Of the 100 pregnant women in the study, 45(45%) were cases. Insulin resistance was found to be significantly more frequent in the cases compared to the controls (adjusted odds ratio=2.78; 95% Confidence Interval: 1.11, 6.90; p<0.01). There was a significant reverse correlation between level of Sex Hormone Binding Globulin in blood and being a case of preeclampsia (adjusted odds ratio=0.99; 95% Confidence Interval: 0.98, 1.00; p=0.04). Conclusion: Independent of insulin resistance, every 1nmol/l increase in Sex Hormone Binding Globulin, decreases the odds of preeclampsia by 1%, notifying Sex Hormone Binding Globulin as an important biomarker about its etiology and prediction. (author)

  9. Proteomic Analysis of Albumins and Globulins from Wheat Variety Chinese Spring and Its Fine Deletion Line 3BS-8

    Directory of Open Access Journals (Sweden)

    Yue-Ming Yan

    2012-10-01

    Full Text Available The relationship between chromosome deletion in wheat and protein expression were investigated using Chinese Spring and fine deletion line 3BS-8. Through 2-DE (2-D electrophoresis analysis, no differentially expressed proteins (DEPs were found in leaf samples; however, 47 DEPs showed at least two-fold abundance variation (p < 0.05 in matured wheat grains and 21 spots were identified by tandem MALDI-TOF/TOF-MS. Among the identified spots, four were cultivar-specific, including three (spots B15, B16, and B21 in Chinese Spring and one in 3BS-8 (spot B10. Among variety-different DEPs between Chinese Spring and 3BS-8, most spots showed a higher express profile in CS; only four spots showed up-regulated expression tendency in 3BS-8. An interesting observation was that more than half of the identified protein spots were involved in storage proteins, of which 11 spots were identified as globulins. According to these results, we can presume that the encoded genes of protein spots B15, B16, and B21 were located on the chromosome segment deleted in 3BS-8.

  10. Purification, crystallization and X-ray diffraction study of basic 7S globulin from soybean

    International Nuclear Information System (INIS)

    Crystallization of basic 7S globulin from soybean is presented. Basic 7S globulin (Bg7S) is expressed by soybeans in response to biotic or abiotic stress. Bg7S is capable of binding to a 4 kDa protein which is supposedly involved in cell proliferation. Bg7S is widely found not only in legumes, but also in other plants; however, its function is still unclear. Here, Bg7S was successfully crystallized. Orthorhombic and monoclinic crystals of Bg7S were obtained under different conditions and belonged to space groups P21212, with unit-cell parameters a = 111.9, b = 130.1, c = 287.8 Å, and P21, with unit-cell parameters a = 85.3, b = 137.6, c = 162.1 Å, ? = 91.2°, respectively

  11. Immunomodulation with rabbit anti-thymocyte globulin in solid organ transplantation.

    Science.gov (United States)

    Ippoliti, Giovanbattista; Lucioni, Marco; Leonardi, Giuseppe; Paulli, Marco

    2015-12-24

    Rabbit anti-thymocyte globulin's manifold mechanisms of action may be attribuited to its polyclonal nature. Its T-cell depleting effect on lymphoid cells is well established: Occurring in the blood and secondary lymphoid tissues, depletion proceeds through complement-dependent lysis, opsonization and apoptotic pathways. Clinical studies have shown that rabbit anti-thymocyte globulin's immunomodulatory effect extends beyond the initial T-cell depletion and up to the period during which lymphocyte populations begin to recover. The drug is able to mediate immunomodulation and graft tolerance by functionally inactivating cell surface receptors involved in antigen recognition, leukocyte trafficking and leukocyte endothelium adhesion. The complex and prolonged immunomodulation induced by this drug contributes to its efficacy in solid organ transplantation, mainly by reducing the incidence of acute graft rejection. PMID:26722653

  12. A simple ligand-binding assay for thyroxine-binding globulin on reusable Sephadex columns

    International Nuclear Information System (INIS)

    A method for the assay of thyroxine-binding globulin on reusable Sephadex G-25 columns is described. It depends upon elution by diluted iodothyronine-free serum of protein-bound [125I]thyroxine from the columns under conditions where binding to thyroxine-binding prealbumin and albumin are abolished. It is simple, rapid and precise, and permits determinations inlarge numbers of samples. Values (mg/l; mean +- S.D.) were: normals 31.6+-5.4, hyperthyroid 28.3+-4.8, hypothyroid 40.6+-7.5, oral contraceptives 40.1+-6.8, pregnant 50.3+-5.4, cirrhotics 20.7+-4.3. Concentrations were reduced in serum heated at 56degC, while the uptake of [125I]triiodothyronine was increased. There was a significant negative correlation between thyroxine-binding globulin concentration and triiodothyronine uptake in the heated serum samples and in euthyroid subjects

  13. N-Glycans Modulate the Function of Human Corticosteroid-Binding Globulin*

    OpenAIRE

    Sumer-Bayraktar, Zeynep; Kolarich, Daniel; Campbell, Matthew P; Ali, Sinan; Packer, Nicolle H.; Thaysen-Andersen, Morten

    2011-01-01

    Human corticosteroid-binding globulin (CBG), a heavily glycosylated protein containing six N-linked glycosylation sites, transports cortisol and other corticosteroids in blood circulation. Here, we investigate the biological importance of the N-glycans of CBG derived from human serum by performing a structural and functional characterization of CBG N-glycosylation. Liquid chromatography-tandem MS-based glycoproteomics and glycomics combined with exoglycosidase treatment revealed 26 complex ty...

  14. A reliable and practicable DCC assay of sex harmone binding globulin

    International Nuclear Information System (INIS)

    A DCC assay of sex hormone binding globulin in plasma with testosterone as the ligand was reported. SHBG was measured with a multiple ligand dose procedure and calculations based on a Scatchard plot. The reliability of the method was assessed by parallelism test, variations within-and between-asssays and comparison with the results assayed with DHT. The method can give reliable results and is practicable for SHBG assay

  15. Corticosteroid-binding globulin in ursids: binding affinities and nutritional regulation

    OpenAIRE

    Chow, Brian A.; Donahue, Seth W.; Marc RL Cattet; Stenhouse, Gordon B

    2011-01-01

    The majority of corticosteroid in circulation in mammals is bound reversibly and with high affinity to a transport protein, corticosteroid-binding globulin (CBG). Cortisol bound to CBG is biologically unavailable to tissues, and consequently, CBG plays a key role in reducing free cortisol concentration and regulating the cellular actions of this hormone, including protecting tissues from potentially deleterious actions. However, little is known about either serum levels of CBG or its role in ...

  16. THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM ?-GLOBULIN

    Directory of Open Access Journals (Sweden)

    S. B. Cheknev

    2014-07-01

    Full Text Available Abstract. Conformational changes of human serum ?-globulin were studied during and after its binding with copper and zinc ions, using molecular ultrafiltration and differential spectrophotometry. The contents of nonbound metals in the filtrate were evaluated, resp., with sodium diethyl thyocarbamate and o-phenanthroline. It has been shown that copper and zinc exhibited common biological properties during their interactions with protein, but the binding differed sufficiently under similar experimental conditions. E.g., it was confirmed that copper was more active at the external sites of ?-globulin molecule, whereas zinc demonstrated tropicity for the areas of protein intraglobular compartments. The metal-binding sites have been described that differ in their parameters of interactions with cations and their spatial location within globular domains. Approaches are suggested for dynamic analysis of saturation for these differently located sites by the metal ions. We discuss the issues of altered conformational state of the ?-globulin molecule during the binding of cations, as well as potential usage of these data in clinical immunology.

  17. COMPARATIVE ELECTROPHORETIC PATTERNS OF ALBUMINS/ GLOBULINS EXTRACTED FROM DRY GRAINS AND GREEN MALTS OF BARLEY VARIETIES

    Directory of Open Access Journals (Sweden)

    Ivica Strelec

    2012-12-01

    Full Text Available Applicability of electrophoretic separations of albumins/globulins, followed by nonspecific protein staining and specific glycoprotein and aminopeptidase detection has been examined for barley variety discrimination. Albumins/globulins extracted from dry grains and green malt of six barley varieties were separated by polyacrylamide gel electrophoresis (10 % T, pH=8.9 of native and sodium dodecyl sulfate denatured proteins, and by isoelectric focusing in pH gradient of 3.5-9.5 and 4.0-6.5. Analysis of dry grain extracts gave better results than green malt extracts. Obtained data indicate that SDS-PAGE of albumins/globulins and IEF in pH gradient 3.5-9.5 followed by Coomassie Blue staining could be useful in Croatian barley variety discrimination. Angora barley could be clearly distinguished from the other varieties which were grouped as follows: Rodnik/Sladoran, Barun/Rex, and Martin. Glycoprotein patterns did not improve the recognition of individual varieties. Broad specificity, phenylalanine and leucine preferring, and arginine specific aminopeptidase were not found as applicable markers for discrimination of examined barley varieties.

  18. Purification and biochemical characterization of 11S globulin from chan (Hyptis suaveolens L. Poit) seeds.

    Science.gov (United States)

    Bojórquez-Velázquez, Esaú; Lino-López, Gisela J; Huerta-Ocampo, José A; Barrera-Pacheco, Alberto; Barba de la Rosa, Ana P; Moreno, Abel; Mancilla-Margalli, N Alejandra; Osuna-Castro, Juan A

    2016-02-01

    Chan (Hyptis suaveolens) is a Mesoamerican crop highly appreciated since the pre-Hispanic cultures. Its proteins are a good source of essential amino acids; however, there are no reports on the properties of its individual proteins. In this study, the 11S globulin (Hs11S) was purified and biochemically characterized. The molecular weight of native Hs11S was about 150-300kDa with isoelectric points of 5.0-5.3, composed by four monomers of 53.5, 52, 51.1 and 49.5kDa, each formed by one acidic subunit and one basic subunit linked by a disulfide bond. Dynamic light scattering, size exclusion chromatography and native PAGE show that Hs11S is assembled in different oligomeric forms. LC-MS/MS analysis confirmed its identity. Hs11S presents antigenic determinants in common with lupin 11S globulin. Carbohydrate moieties or phosphate groups linked to Hs11S were not detected. This information is very useful in order to exploit and utilize rationally chan 11S globulin in food systems. PMID:26304339

  19. The utilization of lupin (Lupinus angustifolius) and faba bean globulins by rats is poorer than of soybean globulins or lactalbumin but the nutritional value of lupin seed meal is lower only than that of lactalbumin.

    Science.gov (United States)

    Rubio, L A; Grant, G; Scislowski, P W; Brown, D; Bardocz, S; Pusztai, A

    1995-08-01

    The effects of dietary sweet lupin (Lupinus angustifolius, Unicrop) seed meal or its insoluble fiber (nonstarch polysaccharides + lignin) on performance, digestibility and nitrogen utilization in growing rats were studied in four experiments. Globulin proteins isolated from lupin, faba bean (Vicia faba L. minor) or soybean (Glycine max) were also incorporated into purified diets as replacements for lactalbumin (control) and the nutritional effects were evaluated. Isocaloric, legume-based diets supplemented with amino acids were used. Final weight gain, gain:feed ratios, nitrogen retention and net protein utilization of the animals fed whole lupin meal-based diets for 10 d were inferior to those of controls. In contrast, adding lupin insoluble fiber to a control diet produced no adverse effects. Ileal starch and apparent nitrogen digestibilities, and fecal digestibility of starch in lupin-fed rats were higher than those of controls, but fecal true nitrogen digestibility was lower. Replacement of lactalbumin with globulin proteins from lupin or faba bean depressed food intake and protein utilization, but only performance was affected by consumption of soybean globulins. Rats consuming lupin or faba bean globulins excreted significantly more nitrogen, particularly as urea through the urine. This did not occur in rats fed soybean globulins. Urea concentration in plasma was higher in rats fed diets containing lupin meal or legume globulins. The concentrations of urea, arginine and ornithine in plasma increased significantly compared with control values after 3 to 9 h of a lupin diet. After 9 h, plasma lysine was also decreased. We concluded that the main reasons for the low nutritional value of sweet lupin seed meal are likely to be related to the chemical structure of the globulin proteins and their adverse effects on growth and nitrogen metabolism, and not to any known antinutritional factor or poor digestibility. PMID:7643249

  20. Conformational characteristics of legume 7S globulins as revealed by circular dichroic, derivative u.v. absorption and fluorescence techniques.

    Science.gov (United States)

    Deshpande, S S; Damodaran, S

    1990-01-01

    The 7S globulin storage proteins, phaseolin, vicilin, and beta-conglycinin of, respectively, dry bean, field pea, and soybean, are highly homologous, have similar predicted protein structures, and yet exhibit considerable differences in their susceptibility to various proteinases [Nielsen, S.S., Deshpande, S.S., Hermodson, M.A. & Scott, M.P. (1988) J. Agric. Food Chem. 36, 896-902]. These differences in their proteolytic behavior were studied in relation to their solution conformational states. The secondary structures of these three proteins determined by far u.v. circular dichroism were characterized by predominantly beta-sheet and beta-turn parameters. However, characterization of tertiary and quaternary structures using second derivative u.v. absorption spectroscopy, surface hydrophobicity using cis-parinaric acid as hydrophobic probe, and fluorescence quenching studies of intrinsic Trp fluorescence using an ionic (iodide) and a neutral (acrylamide) quencher indicated sharp differences in the conformation of these proteins. About 9.6 and 10.2 out of 13 and 15 tyrosyls/subunit of phaseolin and beta-conglycinin, respectively, were exposed to polar solvent, while the surface hydrophobicity varied beta-conglycinin greater than vicilin greater than phaseolin. The Trp residues in phaseolin were not accessible to iodide, while half those of vicilin and beta-conglycinin were quenched. The order of Trp accessibility to acrylamide was vicilin greater than beta-conglycinin greater than phaseolin. The relative compactness of these three proteins based on these studies was related to the observed differences in their susceptibility to various proteinases. PMID:2323881

  1. Protein Pattern Analysis of Blood Globulin to Estimation of Genetic Distance among Population of Tegal, Magelang, and Mojosari Duck

    Directory of Open Access Journals (Sweden)

    AT Sadewo

    2005-11-01

    Full Text Available The research to estimation gene frequency on the protein pattern on blood globulin and to estimation genetic distance among population Tegal, Magelang and Mojosari duck. The material were blood samples from 30 heads of duck. Electroforesis technique was used Sodium Dedocyl Sulfate (SDS polyacrilamide cel elekctroforesis according to Deutcher (1990 method with instrument of polyacrilamide electroforesis vertical system (Mini-protean II, Bio-Rad was used for analysis protein of blood globulin. The gene frequency was estimated according to direct account method and tested for Hardy-Weinberg equilibrium, whereas the genetic distance was estimated by Nei (1972. Result the analysis of electroforesis showed that locus of globulin on the local duck population was controlled by three kind of gene. The gene frequency og GlbA GlbB and GlbC was about 0.05-0.50, migration distance was about 23-60 mm. The genetic distance among Tegal, Magelang and Mojosari duck as 0.010. result of the research was concluded that the protein pattern of blood globulin is usable to estimate both genetic distance and the relationship among population of Tegal, Magelang, and Mojosari duck and also it is estimated that the geographyc location represents a factor that influence the genetic distance. (Animal Production 7(3: 177-184 (2005 Key Words : Blood Globulin, Genetic Distance, Duck Population

  2. Rhesus and African green monkey leukocyte response to steroids, antithymocyte globulin, and 60Co treatments

    International Nuclear Information System (INIS)

    Rhesus and African green monkeys were treated with dexamethasome, antithymocyte globulin, 60Co irradiation, or combinations of those immunosuppressive agents, and the leukocyte response was followed. The lymphocyte population could be significantly depressed with 60Co, but dexamethasone was required to maintain a low lymphocyte count. The ability of the immunosuppressed animals to support the growth of transplanted tumor cells was markedly enhanced by the addition of ATG to the treatment schedule, even though ATG had no effect on the numbers of leukocytes in the peripheral circulation

  3. Testosterone, sex hormone-binding globulin, calculated free testosterone, and oestradiol in male vegans and omnivores.

    OpenAIRE

    Key, TJ; ROE, L; THOROGOOD, M; Moore, JW; Clark, GM; Wang, DY

    1990-01-01

    Total testosterone (T), total oestradiol (E2) and sex hormone-binding globulin (SHBG) concentrations were measured in plasma samples from fifty-one male vegans and fifty-seven omnivores of similar age. Free T concentration was estimated by calculation. In comparison with the omnivores, the vegans had 7% higher total T (P = 0.250), 23% higher SHBG (P = 0.001), 3% lower free T (P = 0.580), and 11% higher E2 (P = 0.194). In a subset of eighteen vegans and twenty-two omnivores for whom 4 d diet r...

  4. Anti-thymocyte Globulin plus Etanercept as Therapy for Myelodysplastic Syndromes (MDS): a Phase II Study

    OpenAIRE

    SCOTT, BART L.; Ramakrishnan, Aravind; Fosdal, Mark; Storer;, Barry; Becker, Pamela; Petersdorf, Steve; Deeg, H.Joachim

    2010-01-01

    Immunosuppressive therapies have proven valuable in treating patients with myelodysplastic syndromes (MDS). We evaluated the combination of equine anti-thymocyte globulin (ATGAM®) and the soluble TNF receptor etanercept (Enbrel®) in a phase II trial. Twenty-five patients with MDS (4-RA, 2-RARS, 15-RCMD, 3-RCMD-RS, 1-RAEB-1) in IPSS risk groups low (n=11) or intermediate-1 (n=14) were enrolled. All patients were platelet or red cell transfusion dependent. Nineteen patients completed therapy wi...

  5. In vitro thyroid testing in populations with low thyroxine binding globulin capacity

    International Nuclear Information System (INIS)

    Total thyroxine (T4) concentration in serum is a reliable indicator of thyroid function in most individuals, but it is affected by altered concentrations of thyroxine-binding globulin (TBG) in serum. Within certain limits, the variations in total TBG binding capacity (TBGTOTAL) caused by the fluctuations in the concentration of this binding globulin in serum can be modulated by calculating the free thyroxine index (FT4I) as the product of T4 and the in vitro uptake of triiodothyronine by a secondary binder (T3U). This calculation is empirically based on the facts that free TBG binding capacity (TBGFREE) is inversely related to T3U and that T4 and T3U show opposite behaviour when measured in sera with altered TBG: a low T4 in serum with reduced TBGTOTAL is compensated by a high value for T3U, while an elevated T4 in serum with increased TBGTOTAL is compensated by a low value for T3U. In both cases the product of T4 and T3 renders a normal FT4I value, showing a certain association with the concentration of free T4 in serum (FT4). In fact, this index has been shown to be superior than several FT4 assay systems in the assessment of thyroid status in clinical euthyroid subjects with relatively high or low T3U

  6. Energy storage

    International Nuclear Information System (INIS)

    This chapter discusses the role that energy storage may have on the energy future of the US. The topics discussed in the chapter include historical aspects of energy storage, thermal energy storage including sensible heat storage, latent heat storage, thermochemical heat storage, and seasonal heat storage, electricity storage including batteries, pumped hydroelectric storage, compressed air energy storage, and superconducting magnetic energy storage, and production and combustion of hydrogen as an energy storage option

  7. Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O

    Directory of Open Access Journals (Sweden)

    Anita Esfandiari

    2014-10-01

    Full Text Available This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat, frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.

  8. Genetic evidence that raised sex hormone binding globulin (SHBG) levels reduce the risk of type 2 diabetes

    DEFF Research Database (Denmark)

    Perry, John R B; Weedon, Michael N; Langenberg, Claudia; Jackson, Anne U; Lyssenko, Valeriya; Sparsø, Thomas; Thorleifsson, Gudmar; Grallert, Harald; Ferrucci, Luigi; Maggio, Marcello; Paolisso, Giuseppe; Walker, Mark; Palmer, Colin N A; Payne, Felicity; Young, Elizabeth; Herder, Christian; Narisu, Narisu; Morken, Mario A; Bonnycastle, Lori L; Owen, Katharine R; Shields, Beverley; Knight, Beatrice; Bennett, Amanda; Groves, Christopher J; Ruokonen, Aimo; Jarvelin, Marjo Riitta; Pearson, Ewan; Pascoe, Laura; Ferrannini, Ele; Bornstein, Stefan R; Stringham, Heather M; Scott, Laura J; Kuusisto, Johanna; Nilsson, Peter; Neptin, Malin; Gjesing, Anette P; Pisinger, Charlotta; Lauritzen, Torsten; Sandbaek, Annelli; Sampson, Mike; Zeggini, Magic Ele; Lindgren, Cecilia M; Steinthorsdottir, Valgerdur; Thorsteinsdottir, Unnur; Hansen, Torben; Schwarz, Peter; Illig, Thomas; Laakso, Markku; Stefansson, Kari; Morris, Andrew D; Groop, Leif; Pedersen, Oluf; Boehnke, Michael; Barroso, Inês; Wareham, Nicholas J; Hattersley, Andrew T; McCarthy, Mark I; Frayling, Timothy M

    2009-01-01

    Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions of epidemiologi...

  9. Preparation of anti-CEA and anti-goat ?-globulin sera for radioimmunologic assay of carcinoembryonic antigen

    International Nuclear Information System (INIS)

    Goats were immunized with purified carcinoembryonic antigen, and the suitability of the antisera for clinical assays of carcinoembryonic antigen was characterized. Reactivity of equine sera to goat ?-globulin as a precipitating factor in the radioimmunologic double antibody technique was also evaluated. (author)

  10. Is serum sex hormone-binding globulin a dominant risk factor for metabolic syndrome?

    Science.gov (United States)

    Yang, Yi-Hong; Zhao, Ming-Jia; Zhou, Shan-Jie; Lu, Wen-Hong; Liang, Xiao-Wei; Xiong, Cheng-Liang; Wan, Chang-Chun; Shang, Xue-Jun; Gu, Yi-Qun

    2015-01-01

    This multi-center, cross-sectional study investigated the association between serum testosterone (T) levels, serum sex hormone-binding globulin (SHBG) levels, and the risk of metabolic syndrome (MS) in 3332 adult Chinese men. The prevalence of MS was 34.7%, and men with MS had lower serum levels of total T (TT) and SHBG than those without MS (P smoking and drinking status, and serum TT (odds ratio [OR] 0.962, 95% confidence interval [95% CI] 0.954-0.969, Psmoking and drinking status (OR 0.981, 95% CI 0.960-1.007). Our study reveals that both serum TT and SHBG levels, but not serum FT, are inversely associated with the prevalence of MS and that serum SHBG is an independent and dominant risk factor for MS. PMID:25851658

  11. Energy Storage.

    Science.gov (United States)

    Eaton, William W.

    Described are technological considerations affecting storage of energy, particularly electrical energy. The background and present status of energy storage by batteries, water storage, compressed air storage, flywheels, magnetic storage, hydrogen storage, and thermal storage are discussed followed by a review of development trends. Included are…

  12. NCI-Black-Reiter (NBR) male rats fail to develop renal disease following exposure to agents that induce alpha-2u-globulin (alpha2u) nephropathy

    OpenAIRE

    Dietrich, Daniel R; Swenberg, James A.

    1991-01-01

    The NCI-Black-Reiter (NBR) rat is the only strain of male rat known not to synthesize the hepatic form of the low molecular weight protein, α2u-globulin. In previous studies, NBR rats were shown not to develop renal disease when exposed to decalin, a compound known to induce α2u-globulin nephropathy in other rat strains. The objective of this study was to show that the presence of α2u-globulin (α2u) is essential for the development of this syndrome in rats exposed to 2,2,4-trimethylpentane (T...

  13. The fate of vicilins, 7S storage globulins, in larvae and adult Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae).

    Science.gov (United States)

    Souza, Sheila M; Uchôa, Adriana F; Silva, José R; Samuels, Richard I; Oliveira, Antônia E A; Oliveira, Eliana M; Linhares, Ricardo T; Alexandre, Daniel; Silva, Carlos P

    2010-09-01

    The fate of vicilins ingested by Callosobruchus maculatus and the physiological importance of these proteins in larvae and adults were investigated. Vicilins were quantified by ELISA in the haemolymph and fat body during larval development (2nd to 4th instars), in pupae and adults, as well as in ovaries and eggs. Western blot analysis demonstrated that the majority of absorbed vicilins were degraded in the fat body. Tracing the fate of vicilins using FITC revealed that the FITC-vicilin complex was present inside cells of the fat body of the larvae and in the fat bodies of both male and female adult C. maculatus. Labelled vicilin was also detected in ovocytes and eggs. Based on the results presented here, we propose that following absorption, vicilins accumulate in the fat body, where they are partially degraded. These peptides are retained throughout the development of the insects and eventually are sequestered by the eggs. It is possible that accumulation in the eggs is a defensive strategy against pathogen attack as these peptides are known to have antimicrobial activity. Quantifications performed on internal organs from larvae of C. maculatus exposed to extremely dry seeds demonstrated that the vicilin concentration in the haemolymph and fat body was significantly higher when compared to larvae fed on control seeds. These results suggest that absorbed vicilins may also be involved in the survival of larvae in dry environments. PMID:20230826

  14. Proposal of abolition of the skin sensitivity test before equine rabies immune globulin application

    Directory of Open Access Journals (Sweden)

    CUPO Palmira

    2001-01-01

    Full Text Available An epizootic outbreak of rabies occurred in 1995 in Ribeirão Preto, SP, with 58 cases of animal rabies (54 dogs, 3 cats and 1 bat confirmed by the Pasteur Institute of São Paulo, and one human death. The need to provide care to a large number of people for the application of equine rabies immune globulin (ERIG prevented the execution of the skin sensitivity test (SST and often also the execution of desensitization, procedures routinely used up to that time at the Emergency Unit of the University Hospital of the Faculty of Medicine of Ribeirão Preto, University of São Paulo (EU-UHFMRP-USP, a reference hospital for the application of heterologous sera. In view of our positive experience of several years with the abolition of SST and of the use of premedication before the application of antivenom sera, we used a similar schedule for ERIG application. Of the 1489 victims of animal bites, 1054 (71% received ERIG; no patient was submitted to SST and all received intravenously anti-histamines (anti-H1 + anti-H2 and corticosteroids before the procedure. The patients were kept under observation for 60 to 180 minutes and no adverse reaction was observed. On the basis of these results, since December 1995 ERIG application has been decentralized in Ribeirão Preto and has become the responsibility of the Emergency Unit of the University Hospital and the Central Basic Health Unit, where the same routine is used. Since then, 4216 patients have received ERIG (1818 at the Basic Health Unit and 2398 at the EU-UHFMRP, with no problems. The ideal would be the routine use of human rabies immune globulin (HRIG in public health programs, but this is problematic, because of their high cost. However, while this does not occur, the use of SST is no longer justified at the time of application of ERIG, in view of the clinical evidence of low predictive value and low sensitivity of SST involving the application of heterologous sera. It is very important to point out that a negative SST result may lead the health team to a feeling of false safety that no adverse reaction will occur, but this is not true for the anaphylactoid reactions. The decision to use premedication, which is based on knowledge about anaphylaxis and on the pharmacology of the medication used, is left to the judgment of health professionals, who should always be prepared for eventual untoward events.

  15. Elimination of undesirable immunoglobulin contaminants including aggregated IgG from gamma-globulin preparations by jackfruit lectin affinity chromatography.

    Science.gov (United States)

    Kondoh, H; Kobayashi, K

    1988-05-13

    Jackfruit lectin, jacalin, has been characterized to combine with IgA of only IgA1 subclass but not with IgA2 subclass and other immunoglobulins. However, we found in the present study that a lectin from another batch of jackfruit (Jacalin-H) showed a different nature in binding reaction. Jacalin-H combined with immunoglobulins of every class or subclass except monomer IgG. More interestingly, the Jacalin-H also combined with aggregated IgG. By means of the Jacalin-H affinity chromatography, we could efficiently eliminate aggregated IgG as well as immunoglobulins other than monomer IgG from commercial gamma-globulin preparations. Jacalin-H affinity chromatography is easy, inexpensive and efficient in the elimination of undesirable immunoglobulin contaminants in the commercial gamma-globulin preparations. PMID:2454765

  16. Protein Pattern Analysis of Blood Globulin to Estimation of Genetic Distance among Population of Tegal, Magelang, and Mojosari Duck

    OpenAIRE

    AT Sadewo

    2005-01-01

    The research to estimation gene frequency on the protein pattern on blood globulin and to estimation genetic distance among population Tegal, Magelang and Mojosari duck. The material were blood samples from 30 heads of duck. Electroforesis technique was used Sodium Dedocyl Sulfate (SDS) polyacrilamide cel elekctroforesis according to Deutcher (1990) method with instrument of polyacrilamide electroforesis vertical system (Mini-protean II, Bio-Rad) was used for analysis protein of blood globul...

  17. Intravenous immune globulin use in patients with human immunodeficiency virus-related thrombocytopenia who require dental extraction.

    OpenAIRE

    Rarick, M. U.; Burian, P.; Guzman, N.; ESPINA, B.; Montgomery, T.; Jamin, D.; Levine, A.M.

    1991-01-01

    Five patients with human immunodeficiency virus (HIV)-related immune thrombocytopenia who were undergoing dental extraction were treated with intravenous immune globulin (IVIG). All patients received IVIG, 1 gram per kg, the day before the dental extraction and again the day of the dental extraction. Four patients had a previous history of minor clinical bleeding. The median baseline platelet count before extraction was 20 X 10(9) per liter (range 13 to 44). The median peak platelet count was...

  18. A Rapid Release of Corticosteroid-Binding Globulin from the Liver Restrains the Glucocorticoid Hormone Response to Acute Stress

    OpenAIRE

    Qian, Xiaoxiao; Droste, Susanne K.; Gutièrrez-Mecinas, María; Collins, Andrew; Kersanté, Flavie; Reul, Johannes M. H. M.; Linthorst, Astrid C. E.

    2011-01-01

    A strict control of glucocorticoid hormone responses to stress is essential for health. In blood, glucocorticoid hormones are for the largest part bound to corticosteroid-binding globulin (CBG), and just a minor fraction of hormone is free. Only free glucocorticoid hormone is able to exert biological effects, but little is known about its regulation during stress. We found, using a dual-probe in vivo microdialysis method, that in rats, the forced-swim stress-induced rise in free corticosteron...

  19. Effect of weight reduction on insulin sensitivity, sex hormone-binding globulin, sex hormones and gonadotrophins in obese children

    DEFF Research Database (Denmark)

    Birkebaek, N H; Lange, Aksel

    2010-01-01

    Obesity in men is associated with reduced insulin sensitivity and hypoandrogenism, while obesity in women is associated with reduced insulin sensitivity and hyperandrogenism. In children, the effect of obesity and weight reduction on the hypothalamo-pituitary-gonadal axis is rarely investigated. The aim of the present study was to investigate the effect of weight reduction in obese Caucasian children on insulin sensitivity, sex hormone-binding globulin (SHBG), DHEAS and the hypothalamo-pituitary-gonadal axis.

  20. Effect of weight reduction on insulin sensitivity, sex hormone-binding globulin, sex hormones and gonadotrophins in obese children

    DEFF Research Database (Denmark)

    Birkebaek, N H; Lange, A; Holland-Fischer, Peter; Kristensen, K; Rittig, Søren; Vilstrup, Hendrik; Handberg, A; Gronbaek, H

    2010-01-01

    Obesity in men is associated with reduced insulin sensitivity and hypoandrogenism, while obesity in women is associated with reduced insulin sensitivity and hyperandrogenism. In children, the effect of obesity and weight reduction on the hypothalamo-pituitary-gonadal axis is rarely investigated. The aim of the present study was to investigate the effect of weight reduction in obese Caucasian children on insulin sensitivity, sex hormone-binding globulin (SHBG), DHEAS and the hypothalamo-pituitary...

  1. Genetic evidence that raised sex hormone binding globulin (SHBG) levels reduce the risk of type 2 diabetes

    OpenAIRE

    2009-01-01

    Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions of epidemiological associations because genotypes are much less likely to be confounded, biased or influenced by disease processes. Using this Mendelian randomization principle, we selected a common single nucleotid...

  2. Genetic evidence that raised sex hormone binding globulin (SHBG) levels reduce the risk of type 2 diabetes

    DEFF Research Database (Denmark)

    Perry, John R B; Weedon, Michael N; Langenberg, Claudia; Jackson, Anne U; Lyssenko, Valeriya; Sparsø, Thomas; Thorleifsson, Gudmar; Grallert, Harald; Ferrucci, Luigi; Maggio, Marcello; Paolisso, Giuseppe; Walker, Mark; Palmer, Colin N A; Payne, Felicity; Young, Elizabeth; Herder, Christian; Narisu, Narisu; Morken, Mario A; Bonnycastle, Lori L; Owen, Katharine R; Shields, Beverley; Knight, Beatrice; Bennett, Amanda; Groves, Christopher J; Ruokonen, Aimo; Jarvelin, Marjo Riitta; Pearson, Ewan; Pascoe, Laura; Ferrannini, Ele; Bornstein, Stefan R; Stringham, Heather M; Scott, Laura J; Kuusisto, Johanna; Nilsson, Peter; Neptin, Malin; Gjesing, Anette P; Pisinger, Charlotta; Lauritzen, Torsten; Sandbaek, Annelli; Sampson, Mike; Zeggini, Ele; Lindgren, Cecilia M; Steinthorsdottir, Valgerdur; Thorsteinsdottir, Unnur; Hansen, Torben; Schwarz, Peter; Illig, Thomas; Laakso, Markku; Stefansson, Kari; Morris, Andrew D; Groop, Leif; Pedersen, Oluf; Boehnke, Michael; Barroso, Inês; Wareham, Nicholas J; Hattersley, Andrew T; McCarthy, Mark I; Frayling, Timothy M

    2010-01-01

    Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions of epidemiological associations because genotypes are much less likely to be confounded, biased or influenced by disease processes. Using this Mendelian randomization principle, we selected a common single nucleotide p...

  3. Genetic evidence that raised sex hormone binding globulin (SHBG) levels reduce the risk of type 2 diabetes

    DEFF Research Database (Denmark)

    Perry, John R B; Weedon, Michael N; Langenberg, Claudia; Jackson, Anne U; Lyssenko, Valeriya; Sparsø, Thomas; Thorleifsson, Gudmar; Grallert, Harald; Ferrucci, Luigi; Maggio, Marcello; Paolisso, Giuseppe; Walker, Mark; Palmer, Colin N A; Payne, Felicity; Young, Elizabeth; Herder, Christian; Narisu, Narisu; Morken, Mario A; Bonnycastle, Lori L; Owen, Katharine R; Shields, Beverley; Knight, Beatrice; Bennett, Amanda; Groves, Christopher J; Ruokonen, Aimo; Jarvelin, Marjo Riitta; Pearson, Ewan; Pascoe, Laura; Ferrannini, Ele; Bornstein, Stefan R; Stringham, Heather M; Scott, Laura J; Kuusisto, Johanna; Nilsson, Peter; Neptin, Malin; Gjesing, Anette P; Pisinger, Charlotta; Lauritzen, Torsten; Sandbaek, Annelli; Sampson, Mike; Zeggini, Magic Ele; Lindgren, Cecilia M; Steinthorsdottir, Valgerdur; Thorsteinsdottir, Unnur; Hansen, Torben; Schwarz, Peter; Illig, Thomas; Laakso, Markku; Stefansson, Kari; Morris, Andrew D; Groop, Leif; Pedersen, Oluf; Boehnke, Michael; Barroso, Inês; Wareham, Nicholas J; Hattersley, Andrew T; McCarthy, Mark I; Frayling, Timothy M

    2009-01-01

    Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions of epidemiological associations because genotypes are much less likely to be confounded, biased or influenced by disease processes. Using this Mendelian randomization principle, we selected a common single nucleotide p...

  4. Retrospective diagnosis of Q fever in a country abattoir by the use of specific IgM globulin estimations

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, A.M.; Hunt, J.G.

    1981-10-03

    Twenty-two cases of pyrexial illness which occurred amongst workers in a country abattoir were investigated retrospectively for Q fever, brucellosis, and leptospirosis. In 18, the illness was shown to be Q fever. No diagnoses were established for the other four. The demonstration of circulating Q-fever-specific IgM globulin was instrumental in establishing the diagnosis in many of the cases.

  5. Yeast-recombinant hepatitis B vaccine: efficacy with hepatitis B immune globulin in prevention of perinatal hepatitis B virus transmission

    International Nuclear Information System (INIS)

    A yeast-recombinant hepatitis B vaccine was licensed recently by the Food and Drug administration and is now available. To assess the efficacy of the yeast-recombinant vaccine, the authors administered the vaccine in combination with hepatitis B immune globulin to high-risk newborns. If infants whose mothers were positive for both hepatitis B surface antigen and the e antigen receive no immunoprophylaxis, 70% to 90% become infected with the virus, and almost all become chronic carriers. Among infants in this study who received hepatitis B immune globulin at birth and three 5-+g doses of yeast-recombinant hepatitis B vaccine, only 4.8% became chronic carriers, a better than 90% level of protection and a rate that is comparable with that seen with immune globulin and plasma-derived hepatitis B vaccine. Hepatitis surface antigen and antibodies were detected by radioimmunoassay. These data suggest that, in this high-risk setting, the yeast-recombinant vaccine is as effective as the plasma-derived vaccine in preventing hepatitis B virus infection and the chronic carrier state

  6. Serum Thyroxine to Thyroxine-Binding Globulin Ratio in Pregnancy and Newborn

    International Nuclear Information System (INIS)

    To evaluate the diagnostic value of the ratio of serum thyroxine(T4) /thyroxine-binding globulin (TBG) for the thyroid status in pregnancy and newborn serum thyroxine, TBG, triiodothyronine, and free thyroxine levels were radioimmunoassayed in normal pregnant women at each of the trimesters, and the calculated serum T4/TBG ratios were compared with other parameters such as T3/TBG ratio and free T4/TBG ratio and free T4/TBG ratio. Serum T4 levels were elevated with the proportionate increase in TBG levels during pregnancy, leading to the nearly constant value of serum T4/TBG ratios as in normal non-pregnant controls. In contrast, serum T3/TBG and free T4/TBG ratios varied considerably during pregnancy. In newborn, T4 levels were nearly not changed with compared non-pregnant control value and TBG levels were elevated. The results indicate that serum T4/TBG ratio is a better parameter than others in evaluating the thyroid status during pregnancy and but newborn is a no better.

  7. Corticosteroid-Binding Globulin: Structure-Function Implications from Species Differences

    Science.gov (United States)

    Gardill, Bernd R.; Vogl, Michael R.; Lin, Hai-Yan; Hammond, Geoffrey L.; Muller, Yves A.

    2012-01-01

    Corticosteroid-binding globulin (CBG) transports glucocorticoids and progesterone in the blood and thereby modulates the tissue availability of these hormones. As a member of the serine protease inhibitor (SERPIN) family, CBG displays a reactive center loop (RCL) that is targeted by proteinases. Cleavage of the RCL is thought to trigger a SERPIN-typical stressed-to-relaxed (S-to-R) transition that leads to marked structural rearrangements and a reduced steroid-binding affinity. To characterize structure-function relationships in CBG we studied various conformational states of E. coli-produced rat and human CBG. In the 2.5 Å crystal structure of human CBG in complex with progesterone, the RCL is cleaved at a novel site that differs from the known human neutrophil elastase recognition site. Although the cleaved RCL segment is five residues longer than anticipated, it becomes an integral part of ?-sheet A as a result of the S-to-R transition. The atomic interactions observed between progesterone and CBG explain the lower affinity of progesterone in comparison to corticosteroids. Surprisingly, CD measurements in combination with thermal unfolding experiments show that rat CBG fails to undergo an S-to-R transition upon proteolytic cleavage of the RCL hinting that the S-to-R transition observed in human CBG is not a prerequisite for CBG function in rat. This observation cautions against drawing general conclusions about molecular mechanisms by comparing and merging structural data from different species. PMID:23300763

  8. In silico identification of anthropogenic chemicals as ligands of zebrafish sex hormone binding globulin

    International Nuclear Information System (INIS)

    Anthropogenic compounds with the capacity to interact with the steroid-binding site of sex hormone binding globulin (SHBG) pose health risks to humans and other vertebrates including fish. Building on studies of human SHBG, we have applied in silico drug discovery methods to identify potential binders for SHBG in zebrafish (Danio rerio) as a model aquatic organism. Computational methods, including; homology modeling, molecular dynamics simulations, virtual screening, and 3D QSAR analysis, successfully identified 6 non-steroidal substances from the ZINC chemical database that bind to zebrafish SHBG (zfSHBG) with low-micromolar to nanomolar affinities, as determined by a competitive ligand-binding assay. We also screened 80,000 commercial substances listed by the European Chemicals Bureau and Environment Canada, and 6 non-steroidal hits from this in silico screen were tested experimentally for zfSHBG binding. All 6 of these compounds displaced the [3H]5α-dihydrotestosterone used as labeled ligand in the zfSHBG screening assay when tested at a 33 μM concentration, and 3 of them (hexestrol, 4-tert-octylcatechol, and dihydrobenzo(a)pyren-7(8H)-one) bind to zfSHBG in the micromolar range. The study demonstrates the feasibility of large-scale in silico screening of anthropogenic compounds that may disrupt or highjack functionally important protein:ligand interactions. Such studies could increase the awareness of hazards posed by existing commercial chemicals at relatively low cost

  9. Induction with Rabbit Antithymocyte Globulin following Orthotopic Liver Transplantation for Hepatitis C

    Directory of Open Access Journals (Sweden)

    R. F. Saidi

    2011-10-01

    Full Text Available Background: Hepatitis C (HCV is the most common indication for liver transplantation in the US. Objective: Since steroids are the major stimulus of viral replication, we postulated that steroid-free immu-nosuppression might be a safer approach. Methods: From January 1995 to October 2002, we used steroid plus calcineurin inhibitor (CNI immuno-suppression after liver transplantation for HCV (steroid group, n=81. From October 2002 to June 2007, rabbit antithymocyte globulin (RATG induction, followed by CNI and azathioprine (RATG group, n=73 was utilized. Results: There were no differences in 1- and 3-year patient/allograft survival rates. The incidence of acute rejection rate (19% vs. 28%, of biopsy-proven HCV recurrence (70% vs. 75%, and chronic rejection (6% vs. 9% were comparable. The mean time to develop recurrent HCV was significantly longer in the RATG group (16.2 vs. 9.2 months, p=0.008. The incidence of severe portal fibrosis appears to be lower in RATG group compared to the steroid group; 14% vs. 4% (p=0.07. Conclusions: RATG induction is safe and effective after liver transplantation for HCV, but has no impact on the incidence of HCV recurrence and patient/allograft survival. However, a significant delay in time to HCV recurrence and a trend toward less rejection and portal fibrosis was observed.

  10. Relation of cigarette smoking in males of different ages to sex hormone binding globulin and testosterone

    International Nuclear Information System (INIS)

    The relationship of cigarette smoking, age, total testosterone free testosterone and sex hormone binding globulin (SHBG) were examined by solid phase radioimmunoassay in 90 randomly chosen healthy males of different ages. The serum levels of these hormones were investigated for smokers compared with non-smokers, of the same ages in 3 groups (adolescent males, middle aged males, and old aged males). Results indicated that cigarette smokers showed increased serum levels of testosterone (60.0% higher, P> 0.05), free testosterone (51.0 higher, P > 0.005) in young adolescent males group, testosterone (27.8% higher, P > 0.001), free testosterone (21.3% higher, P > 0.001) in middle aged males group, and testosterone (21.0% higher, P > 0.001), free testosterone (16.8% higher, P > 0.4) in old ages males group. SHBG was calculated as a mean of free and total testosterone in each group. smokers showed higher mean values of SHBG than non-smokers. Age was positively associated with serum SHBG, it was found that SHBG increased by 17.2% from the youngest (> 18 years) to the oldest age (> 65 years)

  11. Successful desensitization of a patient with aplastic anemia to antithymocyte globulin.

    Science.gov (United States)

    Wolanin, Stephanie A; Demain, Jeffrey G; Meier, Eric A

    2015-01-01

    Antithymocyte globulin (ATG) is a polyclonal gamma immunoglobulin derived from either rabbit or equine serum that serves as therapy for aplastic anemia; however, ATG causes serum sickness in up to 70% and anaphylaxis in up to 5% of recipients. Intradermal (ID) skin testing has been the primary technique used to evaluate for a preexisting Gell and Coombs type I hypersensitivity reaction to ATG. There are no data reporting the predictive value of delayed reactions to ID testing on the risk of serum sickness. This study was designed to establish the importance of epicutaneous and ID skin testing before the administration of ATG through a case report and literature discussion. We report a patient with severe aplastic anemia that was successfully desensitized to ATG after a negative epicutaneous skin test and positive ID skin test. The patient had neither systemic nor localized reactions during the desensitization. Desensitization to ATG in patients with positive epicutaneous skin testing has been shown to be associated with serious and potentially life-threatening complications and should only be considered when the benefits outweigh the risks. Epicutaneous skin testing should be considered in conjunction with ID skin testing when screening for potential sensitivity to ATG. Because of the serious risk of anaphylaxis, desensitization should be performed in an intensive care unit setting in conjunction with a physician familiar with drug desensitization and the management of anaphylaxis. PMID:25730287

  12. Grafting of different glycosides on the surface of liposomes and its effect on the tissue distribution of 125I-labelled ?-globulin encapsulated in liposomes

    International Nuclear Information System (INIS)

    Different glycosides were grafted on the surface of liposomes containing 125I-labelled ?-globulin by two ways: (1) by using glycolipid and (2) by covalent coupling of p-aminophenyl-D-glycosides to phosphatidylethanolamine liposomes using glutaraldehyde. The distribution of 125I-labelled ?-globulin was determined in mouse tissues from 5-60 min after a single injection of these liposomes. The liver uptake of encapsulated 125I-labelled ?-globulin was highest from liposomes having galactose and mannose on the surface. Competition experiments and cross-inhibition studies indicate that this uptake is mediated by specific recognition of the surface galactose and mannose residues of liposomes by the receptors present on the plasma membrane of liver cells. Stearylamine-containing liposomes were found to be more efficient in mediating the uptake of 125I-labelled ?-globulin by the lung, whereas in the case of spleen, phosphatidylethanolamine liposomes were more efficient. The extent of uptake of 125I-labelled ?-globulin from all types of liposome decreases as the amount of given liposomes increases. The uptake of 125I-labelled ?-globulin from liposomes containing asialogangliosides depends upon the phospholipid/glycolipid ratio. These experiments clearly demonstrate that enhanced liposome uptake by liver cells could be achieved by grafting galactose and mannose on the liposomal surface. (orig.)

  13. Associations between sex hormone binding globulin and metabolic syndrome parameters in premenopausal obese women

    Directory of Open Access Journals (Sweden)

    Akin Fulya

    2008-10-01

    Full Text Available Background and Aims: The aim of this study was to determine sex hormone binding globulin (SHBG concentrations in premenopausal obese women and to evaluate the relationships between sex hormones and features of the metabolic syndrome (MetS. Settings and Design: Retrospective cross-sectional analysis was carried out on 350 obese patients aged 25 to 69 years referred to the Department of Endocrinology, Pamukkale University in 2002-2003. Materials and Methods: 125 premenopausal euthyroid patients were eligible for this study. Subjects were divided into two groups according to the body mass index (BMI: Group I, women with BMI < 30 kg/m 2 (n = 17 and Group II,, women with BMI ? 30 kg/m 2 (n = 108. Median SHBG concentration of Group I was 50.1 nmol/L. Group II was divided into two subgroups according to the median SHBG concentration of Group I: subjects with high SHBG levels (SHBG concentration ? median level of the control group, i.e ? 50.1 nmol/L and subjects with low SHBG levels (< 50.1 nmol/L. All statistical analyses were performed using SPSS 9.0 software (SPSS Inc.. Results: No significant difference was found in mean age between the low and high SHBG groups. The low SHBG group was significantly heavier, and with higher waist circumference than the high SHBG group. In the low SHBG group, fasting glucose, postprandial glucose and gamma glutamyl transferase (GGT and free androgen index (FAI were significantly higher. Lipid profile, blood pressure, uric acid, insulin and HOMA were found similar between two groups. Linear regression analyses revealed that body mass index and FAI were significant, being independent predictors of SHBG concentrations in premenopausal women. (r = 0.365, r square = 0.134. Conclusions: It is concluded that low SHBG concentrations may indicate visceral obesity and glucose intolerance in premenopausal women.

  14. Effects of corticosterone pellets on baseline and stress-induced corticosterone and corticosteroid-binding-globulin.

    Science.gov (United States)

    Müller, Claudia; Almasi, Bettina; Roulin, Alexandre; Breuner, Creagh W; Jenni-Eiermann, Susanne; Jenni, Lukas

    2009-01-01

    Exogenous administration of glucocorticoids is a widely used and efficient tool to investigate the effects of elevated concentrations of these hormones in field studies. Because the effects of corticosterone are dose and duration-dependent, the exact course of plasma corticosterone levels after exogenous administration needs to be known. We tested the performance of self-degradable corticosterone pellets (implanted under the skin) in elevating plasma corticosterone levels. We monitored baseline (sampled within 3min after capture) total corticosterone levels and investigated potential interactions with corticosteroid-binding-globulin (CBG) capacity and the endogenous corticosterone response to handling in Eurasian kestrel Falco tinnunculus and barn owl Tyto alba nestlings. Corticosterone pellets designed for a 7-day-release in rodents elevated circulating baseline total corticosterone during only 2-3 days compared to placebo-nestlings. Highest levels occurred 1-2days after implantation and levels decreased strongly thereafter. CBG capacity was also increased, resulting in a smaller, but still significant, increase in baseline free corticosterone levels. The release of endogenous corticosterone as a response to handling was strong in placebo-nestlings, but absent 2 and 8 days after corticosterone pellet implantation. This indicates a potential shut-down of the hypothalamo-pituitary-adrenal axis after the 2-3 days of elevated baseline corticosterone levels. 20 days after pellet implantation, the endogenous corticosterone response to handling of nestlings implanted with corticosterone pellets attained similar levels as in placebo-nestlings. Self-degradable pellets proved to be an efficient tool to artificially elevate circulating baseline corticosterone especially in field studies, requiring only one intervention. The resulting peak-like elevation of circulating corticosterone, the concomitant elevation of CBG capacity, and the absence of an endogenous corticosterone response to an acute stressor have to be taken into account. PMID:18996387

  15. The association between sex hormone-binding globulin and type 2 diabetes in Nigerian men

    Directory of Open Access Journals (Sweden)

    Fayefori M. Abbiyesuku

    2013-03-01

    Full Text Available Background: Epidemiological studies have shown that sex hormone-binding globulin (SHBG has a role in glucose homeostasis in both men and women. However, a prospective study on Japanese-American subjects concluded that SHBG was not a significant risk factor in either men or women, suggesting ethnic differences. We were not aware of any evaluation of SHBG in subjects of African ancestry.Objectives: We investigated the association between SHBG and insulin resistance in type 2 diabetic diabetic men in a hospital in Nigeria.Method: Forty-eight male subjects with type 2 diabetes and 20 non-diabetic male subjects were recruited in this cross-sectional hospital-based study by the convenient sampling method.Height and circumferences around the waist and hip were measured to the nearest 0.5 cm and the waist–hip ratio was calculated from this measurement. Weight was measured and body mass index was calculated. Fasting plasma glucose concentration was measured by the glucose oxidase method with a between-run coefficient of variation of 3%. Insulin and SHBG were measured by means of enzyme-linked immunosorbent assay (ELISA.Results: There was a statistically-significant difference between test results for the diabetic and non-diabetic patients. The mean SHBG concentration was higher in the non-diabetic group (42.2 nmol/L than the diabetic group (30.5 nmol/L. A significant inverse association between insulin resistance and SHBG was observed (r = 0.353, p < 0.015.Conclusion: This study supported earlier observations that a significant inverse correlation exists between SHBG and insulin resistance and provides evidence that the relationship may extend to type 2 diabetic men of African ancestry in Nigeria.

  16. A systematic review of intravenous gamma globulin for therapy of acute myocarditis

    Directory of Open Access Journals (Sweden)

    Crumley Ellen

    2005-06-01

    Full Text Available Abstract Background Intravenous gamma globulin (IVGG is commonly used in the management of acute myocarditis. The objective of this study was to systematically review the literature evaluating this practice. Methods We conducted a comprehensive search (electronic databases, trials registries, conference proceedings, reference lists, contact with authors to identify studies evaluating the use of IVGG in adults and children with a clinical or histologically proven diagnosis of myocarditis of possible viral etiology and symptoms of less than six months duration. Two reviewers independently screened the searches, applied inclusion criteria, and graded the evidence. Results Results were described qualitatively; data were not pooled because only one randomized controlled trial (RCT with 62 patients was identified. The RCT showed no benefit with respect to cardiac function, functional outcome, or event-free survival. A small, uncontrolled trial (n = 10 showed significant improvement in LVEF from a mean of 24% to 41% 12 months after IVGG in nine survivors. A retrospective cohort study of pediatric patients showed improvement in cardiac function and a trend towards improved survival in patients receiving IVGG (n = 21 versus historic controls (n = 25. Ten case reports and two case series (total n = 21 described improvement in cardiac function after administration of IVGG; two case reports showed no benefit of IVGG. One case of hemolytic anemia was attributed to IVGG. Conclusion There is insufficient data from methodologically strong studies to recommend routine use of IVGG for acute myocarditis. Future randomized studies that take into account the etiology of acute myocarditis will be required to determine the efficacy of IVGG.

  17. Isoflavone supplementation reduced serum sex hormone-binding globulin concentration in postmenopausal women

    Directory of Open Access Journals (Sweden)

    ML. Edy Parwanto

    2012-04-01

    Full Text Available Introduction The bone loss that occurs with ageng in postmenopausal women is related to a decrease in serum levels of bioavailable estrogen and testosterone, which are mainly bound to sex hormone-binding globulin (SHBG and albumin. Phytoestrogens are thought to exert hormonal effects in the body due to their structural resemblance to 17â-estradiol. The objective of this study was to evaluate the effect of isoflavone supplementation on levels of SHBG in postmenopausal women aged 47- 60 years. Methods A study of pre and post test design with controls was conducted in 70 women aged 47- 60 years. Subjects were randomly divided into 2 groups, the isoflavone group received 100 mg isoflavones/day + calcium 500 mg/day and the control group calcium 500 mg/day for 6 months. Measurement of bone mineral density was performed prior to supplementation, and serum SHBG levels before and after supplementation. Results Supplementation of isoflavones for 6 months reduced the SHBG levels by 31.1% in the isoflavone group (p=0.000, whereas supplementation of calcium for 6 months did not affect the levels of SHBG in the control group (p=0.359. Supplementation of isoflavones for 6 months reduced SHBG levels of postmenopausal women in the isoflavone group with either osteopenia (p=0.028 or osteoporosis (p=0.008. Conclusion Supplementation of isoflavones for 6 months decreased the SHBG levels of postmenopausal women in the isoflavone group with osteopenia and osteoporosis. Our findings suggest that phytoestrogens may significantly decreased SHBG levels in postmenopausal women.

  18. Lack of association between Rh status, Rh immune globulin in pregnancy and autism.

    Science.gov (United States)

    Miles, Judith H; Takahashi, T Nicole

    2007-07-01

    Though causes of autism are considered largely genetic, considerable concern remains that exposure to Rh immune globulin (RhIg), which until 2001 in the United States contained the preservative thimerosal, can cause autism. To determine whether mothers of children with autism are more likely to be Rh negative (Rh(-)) or to have received RhIg preserved with thimerosal, which is 49.6% ethyl mercury, we surveyed families of children with an autism spectrum disorder (ASD) ascertained through a University-based autism clinic considered free of ascertainment biases related to type of autism or severity. Between 2004 and 2006, 305 mothers of 321 children with an ASD agreed to participate in a telephone interview. Analysis of complete records including the blood group status and RhIg exposure of 214 families showed that Rh(-) status is no higher in mothers of children with autism than in the general population, exposure to antepartum RhIg, preserved with thimerosal is no higher for children with autism and pregnancies are no more likely to be Rh incompatible. This was also true for autism subgroups defined by behavioral phenotype, gender, IQ, regressive onset, head circumference, dysmorphology, birth status, essential, or complex phenotype. These findings support the consensus that exposure to ethylmercury in thimerosal is not the cause of the increased prevalence of autism. These data are important not only for parents in this country but also for the international health community where thimerosal continues to be used to preserve multi-dose vials which in turn makes vaccines affordable. PMID:17508426

  19. Thyroxine binding to serum thyronine-binding globulin in thyroidectomized adult and normal neonatal rats

    International Nuclear Information System (INIS)

    The amount of tracer [125I]T4 bound to serum thyronine-binding globulin (TBG) was measured by polyacrylamide gel electrophoresis in adult thyroidectomized (TX) rats and normal 1-day to 4-week-old rat puts. Thyroidectomy was associated with the appearance of significant amounts of [125I]T4 binding to serum TBG in lean rats, but not in obese Zucker rats. Treatment of the TX rats in vivo with replacement doses of T4 prevented this increase in TBG binding, but enrichment of serum from TX rats with T4 did not. Significant amounts of tracer [125I]T4 binding to TBG was present in serum from 1- to 3-week-old normal rat pups, but not in 1-day- or 4-week-old pups. There were significantly higher levels of TBG binding of [125I]T4 in serum from 2-week-old rat pups raised in litters of 16 pups compared to those raised in litters of 4 pups. All manipulations that result in the appearance of TBG in rat serum also result in either weight loss or a slowing in the rate of growth, suggesting that the appearance of TBG in rat serum has a nutritional component. This possibility is further supported by the observations that increases in TBG binding of [125I]T4 are not found in obese Zucker rats fed a low protein-high carbohydrate diet for 14 days or fasted for 7 days, or after thyroidectomy, perhaps owing to the large stores of fuel in the obese rat

  20. What the world's religions teach, applied to vaccines and immune globulins.

    Science.gov (United States)

    Grabenstein, John D

    2013-04-12

    For millennia, humans have sought and found purpose, solace, values, understanding, and fellowship in religious practices. Buddhist nuns performed variolation against smallpox over 1000 years ago. Since Jenner developed vaccination against smallpox in 1796, some people have objected to and declined vaccination, citing various religious reasons. This paper reviews the scriptural, canonical basis for such interpretations, as well as passages that support immunization. Populous faith traditions are considered, including Hinduism, Buddhism, Jainism, Judaism, Christianity, and Islam. Subjects of concern such as blood components, pharmaceutical excipients of porcine or bovine origin, rubella strain RA 27/3, and cell-culture media with remote fetal origins are evaluated against the religious concerns identified. The review identified more than 60 reports or evaluations of vaccine-preventable infectious-disease outbreaks that occurred within religious communities or that spread from them to broader communities. In multiple cases, ostensibly religious reasons to decline immunization actually reflected concerns about vaccine safety or personal beliefs among a social network of people organized around a faith community, rather than theologically based objections per se. Themes favoring vaccine acceptance included transformation of vaccine excipients from their starting material, extensive dilution of components of concern, the medicinal purpose of immunization (in contrast to diet), and lack of alternatives. Other important features included imperatives to preserve health and duty to community (e.g., parent to child, among neighbors). Concern that 'the body is a temple not to be defiled' is contrasted with other teaching and quality-control requirements in manufacturing vaccines and immune globulins. Health professionals who counsel hesitant patients or parents can ask about the basis for concern and how the individual applies religious understanding to decision-making about medical products, explain facts about content and processes, and suggest further dialog with informed religious leaders. Key considerations for observant believers for each populous religion are described. PMID:23499565

  1. Gene amplification as a cause of inherited thyroxine-binding globulin excess in two Japanese families

    Energy Technology Data Exchange (ETDEWEB)

    Mori, Yuichi; Miura, Yoshitaka; Saito, Hidehiko [Toyota Memorial Hospital (Japan)] [and others

    1995-12-01

    T{sub 4}-binding globulin (TBG) is the major thyroid hormone transport protein in man. Inherited abnormalities in the level of serum TBG have been classified as partial deficiency, complete deficiency, and excess. Sequencing analysis of the TBG gene, located on Xq21-22, has uncovered the molecular defects causing partial and complete deficiency. However, the mechanism leading to inherited TBG excess remains unknown. In this study, two Japanese families, F-A and F-T, with inherited TBG excess were analyzed. Serum TBG levels in hemizygous males were 58 and 44 {mu}g/mL, 3- and 2-fold the normal value, respectively. The molecule had normal properties in terms of heat stability and isoelectric focussing pattern. The sequence of the coding region and the promoter activity of the TBG gene were also indistinguishable between hemizygotes and normal subjects. The gene dosage of TBG relative to that of {beta}-globin, which is located on chromosome 11, and Duchenne muscular dystropy, which is located on Xp, was evaluated by coamplification of these target genes using polymerase chain reaction and subsequent quantitation by HPLC. The TBG/{beta}-globin ratios of the affected male and female of F-A were 3.13 and 4.13 times, respectively, that in the normal males. The TBG/Duchenne muscular dystrophy ratios were 2.92 and 2.09 times the normal value, respectively. These results are compatible with three copies of TBG gene on the affected X-chromosome. Similarly, a 2-fold increase in gene dosage was demonstrated in the affected hemizygote of F-T. A 3-fold tandem amplification of the TBG gene was shown by in situ hybridization of prometaphase and interphase chromosomes from the affected male with a biotinylated genomic TBG probe, confirming the gene dosage results. Gene amplification of TBG is the cause of inherited TBG excess in these two families. 35 refs., 3 figs., 2 tabs.

  2. Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

    DEFF Research Database (Denmark)

    Dam, Svend Secher; Thaysen-Andersen, Morten

    2013-01-01

    Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (?20%), pauci-mannosidic (?40%), and complex forms (?40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants ?-1,2-xylose and ?-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectincontaining two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2 (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has the potential to serve as a model system for studying the role of seed proteins and their glycosylation in food allergy.

  3. Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin. Irish Hepatology Research Group.

    LENUS (Irish Health Repository)

    Kenny-Walsh, E

    2012-02-03

    BACKGROUND AND METHODS: In February 1994, batches of anti-D immune globulin used in Ireland during 1977 and 1978 to prevent Rh isoimmunization were found to be contaminated with hepatitis C virus (HCV) from a single infected donor. In March 1994, a national screening program was initiated for all women who had received anti-D immune globulin between 1970 and 1994. Of the 62,667 women who had been screened when this study began, 704 (1.1 percent) had evidence of past or current HCV infection, and 390 of those 704 (55 percent) had positive tests for serum HCV RNA on reverse-transcription-polymerase-chain-reaction analysis. All 390 were offered a referral for clinical assessment and therapy. We evaluated 376 of these 390 women (96 percent); the other 14 were not seen at one of the designated treatment centers. RESULTS: The mean (+\\/-SD) age of the 376 women was 45+\\/-6 years at the time of screening. They had been infected with hepatitis C for about 17 years. A total of 304 women (81 percent) reported symptoms, most commonly fatigue (248 [66 percent]). Serum alanine aminotransferase concentrations were slightly elevated (40 to 99 U per liter) in 176 of 371 women (47 percent), and the concentrations were 100 U per liter or higher in 31 (8 percent). Liver biopsies showed inflammation in 356 of 363 women (98 percent); in most cases the inflammation was slight (41 percent) or moderate (52 percent). Although the biopsy samples from 186 of the 363 women (51 percent) showed evidence of fibrosis, only 7 women (2 percent) had probable or definite cirrhosis. Two of the seven reported excessive alcohol consumption. CONCLUSIONS: Most of the women with HCV infection 17 years after receiving HCV-contaminated anti-D immune globulin had evidence of slight or moderate hepatic inflammation on liver biopsy, about half had fibrosis, and 2 percent had probable or definite cirrhosis.

  4. Association between testosterone, estradiol and sex hormone binding globulin levels in men with type 1 diabetes with nephropathy

    OpenAIRE

    Maric, Christine; Forsblom, Carol; Thorn, Lena; Wadén, Johan; Groop, Per-Henrik

    2010-01-01

    Male sex is a risk factor for development and progression of diabetic nephropathy; however, the relationship between sex hormone levels and diabetic nephropathy in type 1 diabetic men is unknown. This was a prospective follow-up study as part of the nationwide Finnish Diabetic Nephropathy (FinnDiane) Study; 297 patients were followed for 5.9±1.5 years. Serum total testosterone (Tt) and estradiol (Te), calculated free testosterone (cFt) and estradiol (cFe) and sex hormone binding globulin were...

  5. The Presence of alpha2u-Globulin is Necessary for d-Limonene Promotion of Male Rat Kidney Tumors

    OpenAIRE

    Dietrich, Daniel R.; Swenberg, James A

    1991-01-01

    In a 2-year carcinogenesis bioassay, d-limonene (dL) induced kidney tumors in male F344 rats, but not in female F344 rats or either sex of mice. d-Limonene-1,2-oxide, a metabolite of dL, has been shown to bind reversibly to the male rat-specific urinary protein, ?2u-globulin (?2u-G), resulting in an ?2u-G-chemical complex that is more resistant to lysosomal degradation than ?2u-G alone. This reduced degradation of ?2u-G-chemical complex leads to an accumulation of this protein in the proxima...

  6. New aspects of storage protein accumulation in pea seeds

    International Nuclear Information System (INIS)

    Grain legumes are important not only because they rank fifth in world-wide production behind wheat, maize, rice and barley, but also because they have higher protein contents (20-40%) than most other seed types. The goal of our work is to obtain a more complete understanding of the cell biology of legume seed protein accumulation. To this end, we have used immunogold labeling techniques to study the localization of storage proteins in developing seeds of Pisum sativum. This presentation focuses on two novel observations related to the intracellular transport and storage of these seed proteins. Since continuities between the electron-dense cisternae and vacuoles may be disrupted by directional changes in the streaming cytoplasm during fixation, I re-examined the relationship between these two organelles in seeds in which cytoplasmic streaming was inhibited by chilling on ice. In a separate study, the distribution of both globulin (legumin and vicilin) and albumin (PA1) storage proteins in axis tissue of developing seeds was investigated. Using double labeling with 10 and 20 nm colloidal gold, compartmentation of a sulfur-rich albumin (PA1) and a globulin (vicilin) was demonstrated within individual vacuolar protein deposits in cells at the base of the epicotyl

  7. The cortisol response to ACTH in pigs, heritability and influence of corticosteroid-binding globulin.

    Science.gov (United States)

    Larzul, C; Terenina, E; Foury, A; Billon, Y; Louveau, I; Merlot, E; Mormede, P

    2015-12-01

    In the search for biological basis of robustness, this study aimed (i) at the determination of the heritability of the cortisol response to ACTH in juvenile pigs, using restricted maximum likelihood methodology applied to a multiple trait animal model, and (ii) at the study of the relationships between basal and stimulated cortisol levels with corticosteroid-binding globulin (CBG), IGF-I and haptoglobin, all important players in glucose metabolism and production traits. At 6 weeks of age, 298 intact male and female piglets from 30 litters (30 dams and 30 boars) were injected with 250 µg ACTH(1-24) (Synacthen). Blood was taken before ACTH injection to measure basal levels of cortisol, glucose, CBG, IGF-I and haptoglobin, and 60 min later to measure stimulated cortisol levels and glucose. Cortisol increased 2.8-fold after ACTH injection, with a high correlation between basal and stimulated levels (phenotypic correlation, r p=0.539; genetic correlation, r g=0.938). Post-ACTH cortisol levels were highly heritable (h 2=0.684) and could therefore be used for genetic selection of animals with a more reactive hypothalamic-pituitary-adrenocortical axis. CBG binding capacity correlated with cortisol levels measured in basal conditions in males only. No correlation was found between CBG binding capacity and post-ACTH cortisol levels. Basal IGF-I concentration was positively correlated with BW at birth and weaning, and showed a high correlation with CBG binding capacity with a strong sexual dimorphism, the correlation being much higher in males than in females. Basal haptoglobin concentrations were negatively correlated with CBG binding capacity and IGF-I concentrations. Complex relationships were also found between circulating glucose levels and these different variables that have been shown to be related to glucose resistance in humans. These data are therefore valuable for the genetic selection of animals to explore the consequences on production and robustness traits, but also point at pigs as a relevant model to explore the underlying mechanisms of the metabolic syndrome including the contribution of genetic factors. PMID:26302113

  8. Tritium storage

    International Nuclear Information System (INIS)

    This document represents a synthesis relative to tritium storage. After indicating the main storage particularities as regards tritium, storages under gaseous and solid form are after examined before establishing choices as a function of the main criteria. Finally, tritium storage is discussed regarding tritium devices associated to Fusion Reactors and regarding smaller devices

  9. Physicochemical changes taking place in bovine globulins under the influence of gamma irradiation studied by thermal analysis

    International Nuclear Information System (INIS)

    Radiation modification of protein based polymers as well as the development of gamma irradiation techniques as a method of food sterilisation and preservation induces necessity of better recognition of the physicochemical changes occurring in proteins after gamma irradiation. Recently differential scanning calorimetry (DSC) was applied widely in structural studies of proteins. Also thermoanalytical methods (TG, DTG) applied for proteins pyrolysis were also found to be useful in characterisation of proteins structure and the properties of proteins containing tissues. In presented paper, the aforementioned methods were applied for investigation of the gamma irradiation influence on thermal decomposition of gamma and alpha globulins and the results were related to their structural modifications. It has been found, that irradiation influences the course of decomposition of gamma and alpha globulins. Irradiation of solid native proteins result in decreased temperature of decomposition, especially decreased temperature of the last stage. The effects of irradiation performed for water suspensions were clearly more significant. The relatively large differences between decomposition of the irradiated and non-irradiated samples were detected by DSC and thermogravimetry already after irradiation of water suspensions

  10. Resolution of Mild Ganciclovir-Resistant Cytomegalovirus Disease with Reduced-Dose Cidofovir and CMV-Hyperimmune Globulin

    Science.gov (United States)

    Patel, Samir J.; Kuten, Samantha A.; Knight, Richard J.; Hong, Dana M.; Gaber, A. Osama

    2014-01-01

    Ganciclovir-resistant cytomegalovirus (CMV) is associated with significant morbidity in solid organ transplant recipients. Management of ganciclovir-resistant CMV may be complicated by nephrotoxicity which is commonly observed with recommended therapies and/or rejection induced by “indirect” viral effects or reduction of immunosuppression. Herein, we report a series of four high serologic risk (donor CMV positive/recipient CMV negative) kidney transplant patients diagnosed with ganciclovir-resistant CMV disease. All patients initially developed “breakthrough” viremia while still receiving valganciclovir prophylaxis after transplant and were later confirmed to exhibit UL97 mutations after failing to eradicate virus on adequate dosages of valganciclovir. The patients were subsequently and successfully treated with reduced-dose (1-2?mg/kg) cidofovir and CMV-hyperimmune globulin, given in 2-week intervals. In addition, all patients exhibited stable renal function after completion of therapy, and none experienced acute rejection. The combination of reduced-dose cidofovir and CMV-hyperimmune globulin appeared to be a safe and effective regimen in patients with mild disease due to ganciclovir-resistant CMV. PMID:24991428

  11. Polymorphism and inheritance of serum esterases and beta-globulins in the paradise fish (Macropodus opercularis; Anabantidae).

    Science.gov (United States)

    Monostory, Z; Nagy, A; Gervai, J; Csányi, V

    1984-01-01

    Electrophoretic variants of serum esterases and beta-globulins in two subspecies of paradise fish (Macropodus opercularis) were studied. Four esterase loci (Est-1, Est-2, Est-3 and Est-4), a single transferrin (Tf) and another major beta-globulin locus (Bg) were identified by segregational analysis. Est-3 seems to be a monomorphic locus. Three alleles of Est-1, two of Est-2, two of Est-4, four of Tf and two alleles of Bg were found in the laboratory population. None of these loci were closely linked. Electrophoretic patterns of F1 hybrids confirmed the monomeric structures of each of the studied proteins. Allelic segregation at the Tf and Bg loci was normal in F2 and backcross populations. In crosses of the two Macropodus subspecies there were deviations from Mendelian ratios because of missing recombinant esterase phenotypes. Each of these would have been homozygous Est-2f/f. We suppose that Est-2f/f causes lethality in the early phase of development, except in the Est-1c/c, Est-2f/f combination characteristic of the parental subspecies M.o. concolor. PMID:6204560

  12. Molecular characterization of a genetic variant of the steroid hormone-binding globulin gene in heterozygous subjects

    Energy Technology Data Exchange (ETDEWEB)

    Hardy, D.O.; Catterall, J.F. [Population Council, New York, NY (United States); Carino, C. [Instituto National de la Nutricion, Mexico City, MX (United States)] [and others

    1995-04-01

    Steroid hormone-binding globulin in human serum displays different isoelectric focusing (IEF) patterns among individuals, suggesting genetic variation in the gene for this extracellular steroid carrier protein. Analysis of allele frequencies and family studies suggested the existence of two codominant alleles of the gene. Subsequent determination of the molecular basis of a variant of the gene was carried out using DNA from homozygous individuals from a single Belgian family. It was of interest to characterize other variant individuals to determine whether all variants identified by IEF phenotyping were caused by the same mutation or whether other mutations occurred in the gene in different populations. Previous studies identified Mexican subjects who were heterozygous for the variant IEF phenotype. Denaturing gradient gel electrophoresis was used to localize the mutation in these subjects and to purify the variant allele for DNA sequence analysis. The results show that the mutation in this population is identical to that identified in the Belgian family, and no other mutations were detected in the gene. These data represent the first analysis of steroid hormone-binding globulin gene variation in heterozygous subjects and further support the conclusion of biallelism of the gene worldwide. 11 refs., 2 figs., 1 tab.

  13. Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK) kernel globulin Caracterizaçao funcional das globulinas de amêndoa de castanha-do-Pará após a acetilação

    OpenAIRE

    Cíntia Maria Pinto Ramos; Pushkar Singh Bora

    2004-01-01

    Defatted Brazil nut kernel flour, a rich source of high quality proteins, is presently being utilized in the formulation of animal feeds. One of the possible ways to improve its utilization for human consumption is through improvement in its functional properties. In the present study, changes in some of the functional properties of Brazil nut kernel globulin were evaluated after acetylation at 58.6, 66.2 and 75.3% levels. The solubility of acetylated globulin was improved above pH 6.0 but wa...

  14. Isoforms of thyroxine-binding globulin as a model for molecular epidemiology of human cancer risk

    International Nuclear Information System (INIS)

    The novel field of molecular epidemiology of human cancer risk has added a new branch to classical epidemiology by providing a direct link between human cancer and carcinogen exposure. It was estimated that about 80% of cancers are due to environmental factors. The blood proteins are almost certainly targets for modification in human cancer, and their identification and characterization will be of primary importance in the development of the new and rapidly evolving field of molecular epidemiology. Among blood proteins that are altered in human cancer, TBG occupies a special place because the level of human blood TBG is the most sensitive to intensification of biosynthesis and proliferation processes in organisms in different types of cancer. The increase of TBG concentration in cancer can be result from both activation of TBG biosynthesis in liver or altering of post translation glycosylation that prolongs protein survival time. The molecular basis for the change in the properties of TBG in cancer is unknown. These distinctive changes could have important consequences for the function of TBG in cancer and may help to develop more precise markers for monitoring pathological progression in this disease. Considerable variability and subtlety can occur in the carbohydrate composition and structure of serum glycoproteins in disease. This can be either as a major change, such as an increase in the number of oligosaccharide branches at a particular glycosylation site or as a minor change such as the addition of an extra fucose or sialic acid residue. Increased fucosylation has also been reported for transferrin and alpha-fetoprotein in liver cancer; thyroglobulin in thyroid cancer, IgG in myeloma, haptoglobin in ovarian cancer. The last own studies have shown that in clinically healthy teenagers born in Khojniki (137 Cs 185-555 kBq/m), we have found an unusual thyroid profile exhibiting increased levels of total triiodothyronine (T3), total thyroxine (T4), and thyroxine-binding globulin (TBG) by 50-60%, but no elevation in free T4, as compared with similar control groups of from Minsk. One of the reasons for the euthyroid syndrome in teenagers from the contaminated region was shown to be the blood isoforms in TBG. These isoforms differed in the structure of the carbohydrate components and as a rule are characterized by a prolonged circulation time. Using specific affinity chromatography, with subsequent immunological assay we shown that a certain amount of serum TBG-molecules in persons from Khojniki contains a high level of TBG (about 30% higher than the control group) containing fucosyalated biantennary sugar chains with more prolonged survival time. Previous findings showed that increased levels of fucosylation are observed for some serum glycoproteins in the blood of tumour patients. We provide evidence for variations in the TBGfuc which are contained in the serum TBG pool of patients suffering from cancer with various localisation. Thus, analysis of TBGfuc may be regarded as a prognostic marker in the determination of risk groups. The molecular mechanism of increased activity of fucosyl transferases will be discussed. We propose to measure the level of TBG fucosylated to assess the human cancer risk and habitants of contaminated region of Belarus. (authors)

  15. Underground storage

    International Nuclear Information System (INIS)

    This meeting is divided into three parts: In the first part, 9 papers describe the problems connected with petroleum, natural gas or liquefied gas storage in geologic deposits. In the second part, the problems connected with radioactive waste disposal in deep geologic deposits are described. 10 papers are in the INIS scope. In the third part, another forms of storage are studied (hazardous materials storage, heat storage)

  16. LENTIL TANNIN-GLOBULIN INTERACTIONAND AND IN VITRO HYDROLYSIS INTERAÇÃO TANINO-GLOBULINA DE LENTILHA E HIDRÓLISE IN VITRO

    Directory of Open Access Journals (Sweden)

    Valdir A. NEVES

    1998-08-01

    Full Text Available Protein fractions were isolated from lentil cotyledons and tannins were isolated and purified from lentil seed coats. The globulin fraction corresponded to 42.7% of the total lentil flour nitrogen, representing the major protein fraction. Acetone:water (7:3 was the best extractant for seed coat tannins compared to methanol or methanol-HCl 1%. Native and heated (99oC/15 min. isolated lentil globulin and casein were hydrolyzed with trypsin and pepsin in the absence of tannins and at 1:40, 1:20, 1:10, 1:5 and 1:2.5 tannin-to-protein ratios. The tryptic and peptic hydrolysis of the unheated proteins were reduced with increasing tannin-to-protein ratios. Unheated casein showed to be more susceptible to trypsin than globulin and the opposite effect was observed with pepsin. Heating followed by tannin interaction and hydrolysis had a more pronounced effect on tryptic than peptic digestion for both proteins.As frações protéicas foram isoladas dos cotiledones e os taninos isolados e purificados da casca da lentilha. A fração globulina correspondeu a 42,7 % do nitrogenio total da farinha de lentilha representando a fração protéica majoritária. Comparativamente ao metanol e metanol-HCl 1% a mistura acetona:água (7:3 representou o melhor meio extrator para os taninos da casca. A fração globulina isolada, nativa e aquecida (99oC/15 min, e caseína foram hidrolisadas com tripsina e pepsina na ausência de taninos e na presença de relações tanino:proteína de 1:40, 1:20, 1:10, 1:5 e 1:2,5. A hidrólise tríptica e péptica das proteínas não-aquecidas foram reduzidas com o aumento da relação tanino-proteína. A caseína não aquecida mostrou ser mais susceptível à tripsina que à globulina, o oposto sendo observado com a pepsina. O aquecimento seguido de interação com os taninos e hidrólise teve um efeito mais pronunciado sobre a digestão com tripsina que com pepsina para ambas proteínas.

  17. Purification, crystallization and initial crystallographic characterization of the Ginkgo biloba 11S seed globulin ginnacin

    International Nuclear Information System (INIS)

    The crystallization of ginnacin, the 11S seed storage protein from G. biloba, is reported. Ginkgo biloba, a well known ‘living fossil’ native to China, is grown worldwide as an ornamental shade plant. Medicinal and nutritional uses of G. biloba in Asia have a long history. However, ginkgo seed proteins have not been well studied at the biochemical and molecular level. In this study, the G. biloba 11S seed storage protein ginnacin was purified by sequential anion-exchange and gel-filtration chromatography. A crystallization screen was performed and well diffracting single crystals were obtained by the vapor-diffusion method. A molecular-replacement structural solution has been obtained. There are six protomers in an asymmetric unit. Structure refinement is currently in progress

  18. Temporal relationships between plasma cortisol, corticosteriod-binding globulin (CBG), and the free cortisol index (FCI) in pigs in response to adrenal stimulation or suppression

    Science.gov (United States)

    In swine, more than 60% of cortisol in circulation is bound to its carrier glycoprotein, porcine corticosteroid-binding globulin (pCBG), and is considered biologically inactive. Limitations exist when only total plasma cortisol levels are used to assess the stress response in swine in situations whe...

  19. Effects of a major androgen-dependent urinary protein,. alpha. 2u-globulin on the pituitary-gonadal axis and hypothalamic monoamines in adult male mice

    Energy Technology Data Exchange (ETDEWEB)

    Ghosh, P.K.; Chandrashekar, V.; Steger, R. Bartke, A. (Southern Illinois Univ., Carbondale (USA))

    1990-01-01

    The purpose of the present study was to evaluate the effects of alpha-2u-globulin, a sex-dependent male rat urinary protein on pituitary-gonadal functions and hypothalamic monamine contents in male mice. Adult male mice, maintained under standardized laboratory conditions were injected subcutaneously with alpha-2u-globulin or with vehicle daily for 14 days and killed 16 h after the last injection. Plasma levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T) and testicular levels of T were measured by radioimmunoassays. The concentrations of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in medial basal hypothalamus (MBH) and anterior hypothalamus (AH) were measured by high performance liquid chromatography. Administration of alpha-2u-globulin led to a significant increase in plasma FSH and LH levels. In the MBH of alpha-2u-globulin treated mice, there were significant elevations of NE, DA and 5-HT contents. In the AH, both DA and 5-HT contents were decreased while NE content remained unaltered.

  20. DETECTION OF IL-1? PRODUCED BY HUMAN BLOOD CELLS IN PRESENCE OF PROTEINS FROM THE ?-GLOBULIN FRACTION AND THEIR COMPLEXES WITH METALS

    Directory of Open Access Journals (Sweden)

    S. B. Cheknev

    2014-07-01

    Full Text Available Present study has shown that a total cytokine pool produced by human blood cells in presence of proteins from the ?-globulin fraction, or their complexes with copper and zinc, as well as with pure copper and zinc cations, contains up to 148.0±14.9 pg interleukin-1? (IL-1? per mL. The ?-globulins modified by copper or zinc binding induce, resp., 2.0 and 1.4-fold less IL-1? than intact proteins (significant by p = 0.1. Meanwhile, the copper-?-globulin proved to be 2.7-fold more active (? < 0.01 IL-1? inducer, as a zinc-protein complex. Pure zinc ions did not induce IL-1? production, whereas induction rates with pure copper ions are 1.6-fold higher (p < 0.05 than with ?-globulin, and 1.45-fold more efficient that PHA stimulation (p < 0.1. A potential participation of copper and zinc cations bound to antibodies from the microenvironment and, thus, transforming Fc regions of antibody molecules, is discussed with regard of regulation of IL-1? production by human blood cells induced by activation of their Fc receptors.

  1. Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride

    International Nuclear Information System (INIS)

    The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl2 at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus. (Auth.)

  2. Long-term outcome of 25 children and adolescents with severe aplastic anemia treated with antithymocyte globulin

    Scientific Electronic Library Online (English)

    C.R., de-Medeiros; R.C., Ribeiro; M.A., Bittencourt; J., Zanis-Neto; R., Pasquini.

    2000-05-01

    Full Text Available Severe aplastic anemia (SAA) is probably an immune-mediated disorder, and immunosuppressive therapy is recommended for patients with no available donor for bone marrow transplant. Between October 1984 and November 1987, 25 consecutive children and adolescents with SAA with no HLA-compatible marrow d [...] onor received equine antithymocyte globulin (ATG) (15 mg kg-1 day-1) for 10 days. The patients were evaluated 6 weeks, 6 months, and 12 months after starting ATG treatment. Thereafter, patients were evaluated yearly until July 1998. Median age was 10 years (range, 1.5-20 years), granulocyte counts on referral ranged from 0.032 to 1.4 x 10(9)/l (median 0.256 x 10(9)/l), and 12 patients had granulocyte counts

  3. Binding of Ochratoxin A to a Urinary Globulin: A New Concept to Account for Gender Difference in Rat Nephrocarcinogenic Responses

    Directory of Open Access Journals (Sweden)

    Judit Nagy

    2008-05-01

    Full Text Available SDS-gradient mini-gel electrophoresis and immunoblotting of urine of rats given ochratoxin A (OTA, showed OTA binding to an α2u-globulin. Perceived potential internalised delivery of OTA to proximal tubule epithelia by the carrier, specific only to adult male rats and augmenting other uptake mechanisms, suggests that some experimental nephrotoxicological data may not be appropriate for human risk assessment. Reexamination of female rat renal tumour histopathology of the NTP high dose OTA study showed all carcinomas were solitary, unilateral, microscopic and clinically insignificant at the 2-year end-stage. The novel concept, when consolidated further from our archived material, may moderate current perceptions of the human risk of traces of dietary OTA.

  4. Serum testosterone, sex hormone-binding globulin and total calcium levels predict the calcaneal speed of sound in men

    Scientific Electronic Library Online (English)

    Kok-Yong, Chin; Ima-Nirwana, Soelaiman; Isa Naina, Mohamed; Wan Zurinah Wan, Ngah.

    2012-08-01

    Full Text Available OBJECTIVES: Variations in sex hormones and the calcium balance can influence bone health in men. The present study aimed to examine the relationship between the calcaneal speed of sound and biochemical determinants of bone mass, such as sex hormones, parathyroid hormones and serum calcium. METHODS: [...] Data from 549 subjects from the Malaysian Aging Male Study, which included Malay and Chinese men aged 20 years and older residing in the Klang Valley, were used for analysis. The subjects' calcaneal speed of sound was measured, and their blood was collected for biochemical analysis. Two sets of multiple regression models were generated for the total/bioavailable testosterone and estradiol to avoid multicollinearity. RESULTS: The multiple regression results revealed that bioavailable testosterone and serum total calcium were significant predictors of the calcaneal speed of sound in the adjusted model. After adjustment for ethnicity and body mass index, only bioavailable testosterone remained significant; the total serum calcium was marginally insignificant. In a separate model, the total testosterone and sex hormone-binding globulin were significant predictors, whereas the total serum calcium was marginally insignificant. After adjustment for ethnicity and body mass index (BMI), the significance persisted for total testosterone and SHBG. After further adjustment for age, none of the serum biochemical determinants was a significant predictor of the calcaneal speed of sound. CONCLUSION: There is a significant age-dependent relationship between the calcaneal speed of sound and total testosterone, bioavailable testosterone and sex hormone-binding globulin in Chinese and Malay men in Malaysia. The relationship between total serum calcium and calcaneal speed of sound is ethnicity-dependent.

  5. d-Limonene-induced male rat-specific nephrotoxicity: Evaluation of the association between d-limonene and alpha 2u-globulin

    International Nuclear Information System (INIS)

    d-Limonene is a naturally occurring monoterpene, which when dosed orally, causes a male rat-specific nephrotoxicity manifested acutely as the exacerbation of protein droplets in proximal tubule cells. Experiments were conducted to examine the retention of [14C]d-limonene in male and female rat kidney, to determine whether d-limonene or one or more of its metabolites associates with the male rat-specific protein, alpha 2u-globulin, and if so, to identify the bound material. The results indicated that, 24 hr after oral administration of 3 mmol d-limonene/kg, the renal concentration of d-limonene equivalents was approximately 2.5 times higher in male rats than in female rats. Equilibrium dialysis in the presence or absence of sodium dodecyl sulfate indicated that approximately 40% of the d-limonene equivalents in male rat kidney associated with proteins in a reversible manner, whereas no significant association was observed between d-limonene equivalents and female rat kidney proteins. Association between d-limonene and male rat kidney proteins was characterized by high-performance gel filtration and reverse-phase chromatography. Gel filtration HPLC indicated that d-limonene in male rat kidney is associated with a protein fraction having a molecular weight of approximately 20,000. Separation of alpha 2u-globulin from other kidney proteins by reverse-phase HPLC indicated that d-limonene associated with a protein present only in male rat kidney which was definitively identified as alpha 2u-globulin by amino acid sequencing. The major metabolite associated with alpha 2u-globulin was d-limonene-1,2-oxide. Parent d-limonene was also identified as a minor component in the alpha 2u-globulin fraction

  6. Effect of phosphorus on the composition and accumulation of 7S and 11S globulin subunits during seed development of three soybean varieties

    Scientific Electronic Library Online (English)

    J, Han; W.G, Jie; B.Y, Cai.

    2014-12-01

    Full Text Available Three soybean varieties (Dongnong 42, high-protein cultivar; Hefeng 25, medium-protein; and Dongnong 46, low-protein cultivar) grown under different P conditions were investigated in order to evaluate the effect of P on the composition and accumulation of 7S and 11S globulin subunits. The soybean se [...] eds were planted in pots and fertilized with 0.033 g of N and K2O per kg soil. Four levels of P treatment were designed, which were P1, P2, P3 and P4 (i.e., 0, 0.033, 0.067, and 0.100 g of phosphorus pentoxide (P2O5) per kilogram of soil). The composition of extractable proteins was determined by conducting SDS-PAGE. No significant differences were observed between the varieties with regard to the molecular weights of the globulin subunits. After the subunits were formed, their concentrations gradually increased under different P treatments, reached a peak at 70 d post-anthesis (DPA), and then declined during maturity. The concentrations of the 7S and 11S globulins and other subunits in Dongnong 42 and Hefeng 25 were the highest under the P3 treatment, while they were the highest in Dongnong 46 under the P2 treatment. Under the same P treatment, the concentrations were high in Dongnong 42 and low in Dongnong 46 and Hefeng 25. Significant differences were observed in the concentrations of 7S and 11S globulin subunits between the varieties and levels of P treatments, especially under the P3 treatment. Alpha subunit was mainly in relation with the P-nutrition in the three varieties. These findings suggested that the; optimal combination of cultivarsand P treatment; levels could contribute to the increased concentrations of both soybean globulins and their subunits.

  7. Effect of phosphorus on the composition and accumulation of 7S and 11S globulin subunits during seed development of three soybean varieties

    Scientific Electronic Library Online (English)

    J, Han; W.G, Jie; B.Y, Cai.

    Full Text Available Three soybean varieties (Dongnong 42, high-protein cultivar; Hefeng 25, medium-protein; and Dongnong 46, low-protein cultivar) grown under different P conditions were investigated in order to evaluate the effect of P on the composition and accumulation of 7S and 11S globulin subunits. The soybean se [...] eds were planted in pots and fertilized with 0.033 g of N and K2O per kg soil. Four levels of P treatment were designed, which were P1, P2, P3 and P4 (i.e., 0, 0.033, 0.067, and 0.100 g of phosphorus pentoxide (P2O5) per kilogram of soil). The composition of extractable proteins was determined by conducting SDS-PAGE. No significant differences were observed between the varieties with regard to the molecular weights of the globulin subunits. After the subunits were formed, their concentrations gradually increased under different P treatments, reached a peak at 70 d post-anthesis (DPA), and then declined during maturity. The concentrations of the 7S and 11S globulins and other subunits in Dongnong 42 and Hefeng 25 were the highest under the P3 treatment, while they were the highest in Dongnong 46 under the P2 treatment. Under the same P treatment, the concentrations were high in Dongnong 42 and low in Dongnong 46 and Hefeng 25. Significant differences were observed in the concentrations of 7S and 11S globulin subunits between the varieties and levels of P treatments, especially under the P3 treatment. Alpha subunit was mainly in relation with the P-nutrition in the three varieties. These findings suggested that the; optimal combination of cultivarsand P treatment; levels could contribute to the increased concentrations of both soybean globulins and their subunits.

  8. Cask storage

    International Nuclear Information System (INIS)

    Purpose: To eliminate various kinds of economical disadvantages in construction of cask storage and disperse risks caused by massive storage. Constitution: The storage half-underground structure is composed of the cask storage room buried under the ground and the ventilation room with the air inlet and outlet placed on the ground level. Direct radiation from the cask is shielded by using soils below the ground level, and radiation in the ceiling direction is shielded by the ceiling concrete. Heat generated from the cask is transferred and exhausted by natural air ventilation from the air inlet to the outlet by using the drive force of buoyancy generated from the difference of air density between the inside and the outside of the storage. The cask is taken in and out of the shielding lid installed in the ceiling corresponding to the storage position, by using a movable crane. (Sekiya, K.)

  9. Energy storage

    International Nuclear Information System (INIS)

    This book proposes a broad overview of the technologies developed in the domains of on-board electricity storage (batteries, super-capacitors, flywheels), stationary storage (hydraulic dams, compressed air, batteries and hydrogen), and heat storage (sensible, latent and sorption) together with their relative efficiency, their expected developments and what advantages they can offer. Eminent specialists of this domain have participated to the redaction of this book, all being members of the Tuck's Foundation 'IDees' think tank. (J.S.)

  10. Tritium storage

    International Nuclear Information System (INIS)

    A general synthesis about tritium storage is achieved in this paper and a particular attention is given to practical application in the Fusion Technology Program. Tritium, storage under gaseous form and solid form are discussed (characteristics, advantages, disadvantages and equipments). The way of tritium storage is then discussed and a choice established as a function of a logic which takes into account the main working parameters

  11. Energy storage

    CERN Document Server

    Brunet, Yves

    2013-01-01

    Energy storage examines different applications such as electric power generation, transmission and distribution systems, pulsed systems, transportation, buildings and mobile applications. For each of these applications, proper energy storage technologies are foreseen, with their advantages, disadvantages and limits. As electricity cannot be stored cheaply in large quantities, energy has to be stored in another form (chemical, thermal, electromagnetic, mechanical) and then converted back into electric power and/or energy using conversion systems. Most of the storage technologies are examined: b

  12. Effect of long-term storage on hormone measurements in samples from pregnant women: The experience of the Finnish Maternity Cohort

    OpenAIRE

    Holl, Katsiaryna; Lundin, Eva; Kaasila, Marjo; Grankvist, Kjell; Afanasyeva, Yelena; Hallmans, Göran; Lehtinen, Matti; Pukkala, Eero; Surcel, Helja-Marja; Toniolo, Paolo; Zeleniuch-Jacquotte, Anne; Koskela, Pentti; Lukanova, Annekatrin

    2007-01-01

    Validity of biobank studies on hormone associated cancers depend on the extent the sample preservation is affecting the hormone measurements. We investigated the effect of long-term storage (up to 22 years) on immunoassay measurements of three groups of hormones and associated proteins: sex-steroids [estradiol, progesterone, testosterone, dihydroepiandrosterone sulphate (DHEAS), sex hormone-binding globulin (SHBG)], pregnancy-specific hormones [human chorionic gonadotropin (hCG), placental gr...

  13. The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut

    Directory of Open Access Journals (Sweden)

    Sirvent Sofía

    2012-12-01

    Full Text Available Abstract Background The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT, enzyme-linked immunosorbent assay (ELISA, immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes. Conclusions The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2.

  14. The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut

    Science.gov (United States)

    2012-01-01

    Background The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT), enzyme-linked immunosorbent assay (ELISA), immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut) but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes. Conclusions The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2. PMID:23231956

  15. Atomic storage

    CERN Multimedia

    Ricadela, A

    2003-01-01

    IBM is supplying CERN, the European Organization for Nuclear Research, with its Storage Tank file system virtualization software, 20 terabytes of storage capacity, and services under a three-year deal to build computer systems that will support the Large Hadron Collider accelerator (1 paragraph).

  16. Sex hormone binding globulin and risk factors for breast cancer in a population of normal women who had never used exogenous sex hormones.

    OpenAIRE

    Moore, J.W.; Key, T. J.; Bulbrook, R. D.; Clark, G. M.; Allen, D S; Wang, D. Y.; Pike, M.C.

    1987-01-01

    Sex hormone binding globulin (SHBG) concentrations were measured by immunoradiometric assay in serum samples from 1,221 healthy female volunteers aged 34-79 who had never used oral contraceptives or hormone replacement therapy, had no history of thyroid disease or cancer, and had not used any drugs known to influence SHBG in the 14 days preceding blood sampling. There were 616 premenopausal and 605 naturally postmenopausal women. In both premenopausal and postmenopausal women, SHBG decreased ...

  17. Associations of sex hormone-binding globulin and testosterone with diabetes among men and women (the Saku Diabetes study): a case control study

    OpenAIRE

    Goto Atsushi; Morita Akemi; Goto Maki; Sasaki Satoshi; Miyachi Motohiko; Aiba Naomi; Terauchi Yasuo; Noda Mitsuhiko; Watanabe Shaw

    2012-01-01

    Abstract Background Sex hormone-binding globulin (SHBG) levels and sex hormones have been implicated in the pathogenesis of type 2 diabetes and cardiovascular diseases. As fatty liver has been suggested to be a major determinant of SHBG levels, we examined whether the associations of SHBG and testosterone with diabetes were independent of fatty liver. Methods We conducted a case–control study that included 300 diabetes cases (215 men and 85 women) and 300 matched controls from the Saku cohort...

  18. Coffee and Caffeine Consumption in Relation to Sex Hormone–Binding Globulin and Risk of Type 2 Diabetes in Postmenopausal Women

    OpenAIRE

    Goto, Atsushi; Song, Yiqing; Chen, Brian H; Manson, JoAnn Elisabeth; Buring, Julie Elizabeth; Liu, Simin

    2010-01-01

    OBJECTIVE: Coffee consumption has been inversely associated with type 2 diabetes risk, but its mechanisms are largely unknown. We aimed to examine whether plasma levels of sex hormones and sex hormone–binding globulin (SHBG) may account for the inverse association between coffee consumption and type 2 diabetes risk. RESEARCH DESIGN AND METHODS: We conducted a case-control study nested in the prospective Women's Health Study (WHS). During a median follow-up of 10 years, 359 postmenopausal wo...

  19. Hazard evaluation of chemicals that cause accumulation of alpha 2u-globulin, hyaline droplet nephropathy, and tubule neoplasia in the kidneys of male rats.

    OpenAIRE

    Hard, G C; Rodgers, I S; Baetcke, K. P.; Richards, W L; McGaughy, R E; Valcovic, L R

    1993-01-01

    This review paper examines the relationship between chemicals inducing excessive accumulation of alpha 2u-globulin (alpha 2u-g) (CIGA) in hyaline droplets in male rat kidneys and the subsequent development of nephrotoxicity and renal tubule neoplasia in the male rat. This dose-responsive hyaline droplet accumulation distinguishes CIGA carcinogens from classical renal carcinogens. CIGA carcinogens also do not appear to react with DNA and are generally negative in short-term tests for genotoxic...

  20. Failure of adjunctive cytomegalovirus intravenous immune globulin to improve efficacy of ganciclovir in patients with acquired immunodeficiency syndrome and cytomegalovirus retinitis: a phase 1 study.

    OpenAIRE

    Jacobson, M. A.; O'Donnell, J J; Rousell, R; Dionian, B; Mills, J

    1990-01-01

    Six men with acquired immunodeficiency syndrome (AIDS) and cytomegalovirus (CMV) retinitis, treated with combined ganciclovir induction therapy and hyperimmune globulin (CMV-IGIV) for 10 days followed by CMV-IGIV alone, had a median time to retinitis progression shorter (7 days) than had eight historical controls given ganciclovir maintenance therapy (54 days; P = 0.06) and similar to that in eight controls given ganciclovir for 10 days only (19 days; P = 0.97). CMV-IGIV, which also failed to...

  1. Pisum sativum seed globulins as biochemical markers for « wrinkled-smooth » seed character: comparison of genotypes through different protein extraction and electrophoretic procedures

    OpenAIRE

    Rao, R; Pernollet, J Claude

    1981-01-01

    Neuf cultivars, appartenant à quatre classes de génotypes impliquant les gènes ra et rb responsables du caractère lisse-ridé des grains et du caractère simple-composé des grains d’amidon, ont été étudiés par l’électrophorèse des protéines de réserve. Ces protéines ont été extraites soit de moutures, soit de corpuscules protéiques, eux-mêmes obtenus soit à partir de moutures, soit par broyage en milieu humide des cotylédons décortiqués. Les deux fractions de globulines, viciline et légumin...

  2. The Effects of Caffeinated and Decaffeinated Coffee on Sex Hormone-Binding Globulin and Endogenous Sex Hormone Levels: A Randomized Controlled Trial

    OpenAIRE

    Wedick Nicole M; Mantzoros Christos S; Ding Eric L; Brennan Aoife M; Rosner Bernard; Rimm Eric B; Hu Frank B; Van Dam Rob M

    2012-01-01

    Abstract Background Findings from observational studies suggest that sex hormone-binding globulin (SHBG) and endogenous sex hormones may be mediators of the putative relation between coffee consumption and lower risk of type 2 diabetes. The objective of this study was to evaluate the effects of caffeinated and decaffeinated coffee on SHBG and sex hormone levels. Findings After a two-week run-in phase with caffeine abstention, we conducted an 8-week parallel-arm randomized controlled trial. He...

  3. Interaction of blood sex steroid-binding globulin-steroid complexes with the plasma membranes of cells of the human decidual endometrium

    International Nuclear Information System (INIS)

    The plasma membranes of cells of the decidual tissue specifically bind complexes of the sex steroid-binding globulin (SBG) of the blood with estrogens (estradiol, estriol, estrone) and the pharmacological agent danazol but do not interact with SBG-testosterone and SBG-dihydrotestosterone complexes. The selectivity of the interaction of SBG-steroid complexes with the cell membranes of the decidual tissue confirms the hypothesis of an active role of SBG in the action of steroids on this target tissue

  4. Antibodies to glycosylphosphatidyl-inositol anchored proteins (GPI-AP) in antithymocyte and antilymphocyte globulin: possible role for the expansion of GPI-AP deficient cells in aplastic anemia

    OpenAIRE

    Breitinger, Heike H.; Rojewski, Markus T.; Schrezenmeier, Hubert

    2009-01-01

    Abstract Antithymocyte globulin (ATG) and antilymphocyte globulin (ALG) are currently used successfully for immunosuppressive treatment of aplastic anemia. In this study we have investigated whether commercial ATG/ALG preparations contain antibodies against glycosylphosphatidyl-inositol anchored proteins (GPI-AP), which could be responsible for emergence of GPI-deficient populations in aplastic anemia after ATG/ALG therapy. We analyzed four commercial ATG/ALG preparations by compet...

  5. Production of the Growth Factors GM-CSF, G-CSF, and VEGF by Human Peripheral Blood Cells Induced with Metal Complexes of Human Serum ?-Globulin Formed with Copper or Zinc Ions

    OpenAIRE

    Cheknev, Sergey B.; Apresova, Maria A.; Moryakova, Nadezhda A.; Efremova, Irina E.; Mezdrokhina, Anna S.; Piskovskaya, Lidya S.; Babajanz, Alla A.

    2014-01-01

    As it was established in our previous studies, the proteins of human serum ?-globulin fraction could interact with copper or zinc ions distributed in the periglobular space, form metal complexes, and become able to perform effector functions differing due to the conformational shifts from those mediated by them in native conformation of their Fc regions. In the present work we have evaluated ability of the ?-globulin metal complexes formed with copper or zinc ions in the conditions like to th...

  6. [Chemical modification of proteins. 5. Modification of the 11-S-globulin from sunflower seed by reaction with dialdehyde starch].

    Science.gov (United States)

    Schwenke, K D; Linow, K J; Raab, B; Uhlig, J

    1979-01-01

    Dialdehyde starch (DAS) reacts unspecifically with the amino acid residues of the 11 S globulin from sunflower seed. The modification of the protein causes a decrease of the content of each amino acid. Their blocking reaches maximum values at high pH levels (9,5) and high concentration of protein (5%). Especially high reactivity is shown by arginine as well as by the hydrophobic amino acids isoleucine, valine, and proline, and furthermore by histidine, lysine, asparagine (aspartic acid), and glutamine (glutamic acid). By reaction with DAS at pH 8.0 70% of the amino groups are blocked within 6 h; on the contrary, glyoxale blocks only 30% of the amino groups. Owing to the blockage of charged amino acid groups, a shift of the isoelectric point of the protein to a lower pH (4,3-4,4) takes place; this effect can be followed for 2 days. As a result of the reaction with DAS, only small amounts (10-15%) of intermolecular crosslinkage products with sedimentation coefficients of 17 S and greater than 17 S were formed. But by means of SDS-gel electrophoresis, dimers and trimers of the polypeptide chains in the protein were detected. PMID:471038

  7. Globulina antilinfocítica en el tratamiento de la anemia aplásica severa / Antilymphocyte globulin in the treatment of severe aplastic anemia

    Scientific Electronic Library Online (English)

    Víctor, Ulloa Perez; Wilson, Ruiz Gil; Luz, Bustamante; Carolina, Tokumura Tokumura.

    1999-10-01

    Full Text Available Objetivo: Describir la experiencia en nuestro hospital en el tratamiento de anemia aplásica severa con globulina antilinfocítica. Materiales y Metodos: Se estudiaron 58 pacientes con AAS diagnosticados entre 1988-1998 de los cuales 25 recibieron Globulina antilinfocítica (GAL), 56% de ellos eran men [...] ores a 20 años de edad,19 de los pacientes tratados con GAL son del sexo masculino . Resultado: Respondieron favorablemente 68%(17pacientes),la mayoría de ellos (64%)alcanzaron esta respuesta durante los primeros 6 meses. Un paciente evolucionó a Hemoglobinuria paroxística nocturna (HPN), efectos adversos fueron leves y de corta duración. (Rev Med Hered;1999 ;10:132 - 136 ). Abstract in english Objetive: To report the experience with antilymphocyte globulin in the treatment of severe aplastic anemia. Material and methods: We studied 58 patients with SAA between 1988-1998. Twenty five patients were treated with ALG, 56 % of them were less than 20 years old, nineteen of the patients were mal [...] es. Results:We observed good response in 68% of the patients, the majority of the patients (64%) got a good response during the first 6 months of the follow up. Only one patient evolved into Paroxysmal Nocturnal Hemoglobinuria. Side effects were mild and short in duration. (Rev Med Hered; 1999;10:132 - 136 ).

  8. Methyl isobutyl ketone (MIBK) induction of ?2u-globulin nephropathy in male, but not female rats

    International Nuclear Information System (INIS)

    Male F-344 rats were administered corn oil (vehicle control), d-limonene (positive control, 300 mg/kg), or MIBK (1000 mg/kg) and female F-344 rats corn oil (vehicle control) or MIBK for 10 consecutive days by oral gavage. Approximately 24 h after the final dose the kidneys were excised and the left kidney prepared and evaluated for histological changes including protein (hyaline) droplet accumulation, immunohistochemical staining for ?2u-globulin (?2u), and proliferating cell nuclear antigen (PCNA) to quantitate renal cell proliferation. The right kidney was prepared for quantitation of total protein and ?2u using an ELISA. MIBK elicited an increase in protein droplets, accumulation of ?2u, and renal cell proliferation in male, but not female rats, responses characteristic of ?2u-mediated nephropathy. MIBK produced identical histopathological changes in the male rat kidney when compared to d-limonene, an acknowledged inducer of ?2u-nephropathy except that the grade of severity tended to be slightly lower with MIBK. MIBK did not induce any effects in female rats. Therefore, renal histopathology, along with the other measures of ?2u accumulation, provides additional weight of evidence to support the inclusion of MIBK in the category of chemicals exerting renal effects through a ?2u-nephropathy-mediated mode-of-action

  9. The COX-2 gene promoter polymorphism -765 delays CD4 T-cell reconstitution after lymphocyte depletion with antithymocyte globulins.

    Science.gov (United States)

    Courivaud, Cécile; Bamoulid, Jamal; Ferrand, Christophe; Tiberghien, Pierre; Chalopin, Jean-Marc; Saas, Philippe; Ducloux, Didier

    2011-11-01

    Polyclonal antithymocyte globulins (ATG) induce persistent changes in T-lymphocyte subsets characterized by low CD4 T. The mechanisms remain partly unknown. Prostaglandin E(2) (PGE(2)) is involved in lymphocyte homeostasis. Whether PGE(2) may be involved in persistent CD4 T-cell lymphopenia after ATG is unknown. We examined the association between this polymorphism and CD4 T-cell count in 159 renal transplant recipients (RTR) who received ATG. Analysis of these patients identified 6 CC (3.8%), 32 GC (22.6%), and 117 GG (73.6%) genotypes. Patients with the GG genotype had significantly higher serum PGE(2) concentrations, leading us to compare C carriers with GG patients. Carriers of the C allele had lower CD4 T cell count 1 year (235 ± 96 vs 323 ± 227/mm(3); p = 0.022) and 2 years posttransplant (325 ± 79 vs 422 ± 231/mm(3); p = 0.024). In multivariate analysis, the C allele (p = 0.029) conferred an increased risk of posttransplant CD4 T-cell lymphocytopenia. Pretransplant T-cell receptor excision circle levels were lower in C carriers. COX-2 gene promoter polymorphism at position -765 (G ? C) is associated with persistent CD4 T-cell lymphopenia after ATG in RTR. This effect is likely to be mediated by the actions of PGE(2) on thymus function and viability. PMID:21782872

  10. Heat-denaturation and aggregation of quinoa (Chenopodium quinoa) globulins as affected by the pH value.

    Science.gov (United States)

    Mäkinen, Outi E; Zannini, Emanuele; Koehler, Peter; Arendt, Elke K

    2016-04-01

    The influence of heating (100°C; 0-15min) on the relative molecular mass, protein unfolding and secondary structure of quinoa globulins was studied at pH 6.5 (low solubility), 8.5 and 10.5 (high solubility). The patterns of denaturation and aggregation varied with pH. Heating triggered the disruption of the disulfide bonds connecting the acidic and basic chains of the chenopodin subunits at pH 8.5 and 10.5, but not at pH 6.5. Large aggregates unable to enter a 4% SDS-PAGE gel were formed at pH 6.5 and 8.5, which became soluble under reducing conditions. Heating at pH 10.5 lead to a rapid dissociation of the native chenopodin and to the disruption of the subunits, but no SDS-insoluble aggregates were formed. No major changes in secondary structure occurred during a 15min heating, but an increase in hydrophobicity indicated unfolding of the tertiary structure in all samples. PMID:26593460

  11. Total Lymphoid Irradiatione—Antithymocyte Globulin Conditioning and Allogeneic Transplantation for Patients with Myelodysplastic Syndromes and Myeloproliferative Neoplasms

    Science.gov (United States)

    Benjamin, Jonathan; Chhabra, Saurabh; Kohrt, Holbrook E.; Lavori, Philip; Laport, Ginna G.; Arai, Sally; Johnston, Laura; Miklos, David B.; Shizuru, Judith A.; Weng, Wen-Kai; Negrin, Robert S.; Lowsky, Robert

    2015-01-01

    Allogeneic hematopoietic cell transplantation (allo HCT) is the only curative therapy for the myelodysplastic syndromes (MDS) and myeloproliferative neoplasms (MPN), but treatment toxicity has been a barrier to its more widespread use. The nonmyeloablative regimen of total lymphoid irradiation (TLI) and antithymocyte globulin (ATG) permits the establishment of donor hematopoiesis necessary for the graft-versus-malignancy effect and is protective against acute graft-versus-host disease (aGVHD), but it has minimal direct cytotoxicity against myeloid diseases. We explored the use of TLI-ATG conditioning to treat 61 patients with allo HCT for MDS (n = 32), therapy-related myeloid neoplasms (n = 15), MPN (n = 9), and chronic myelomonocytic leukemia (n = 5). The median age of all patients was 63 years (range, 50 to 73). The cumulative incidence of aGVHD grades II to IV was 14% (95% confidence interval [CI], 4% to 23%) and for grades III to IV, 4% (95% CI, 0 to 9%), and it did not differ between patients who received allografts from related or unrelated donors. The cumulative incidence of nonrelapse mortality (NRM) at 100 days, 12 months, and 36 months was 0%, 7%, and 11%. Overall survival and progression-free survival were 41% (95% CI, 29% to 53%) and 35% (95% CI, 23% to 48%), respectively. The safety and tolerability of TLI-ATG, as exemplified by its low NRM, provides a foundation for further risk-adapted or prophylactic interventions to prevent disease progression. PMID:24607552

  12. Total lymphoid irradiation-antithymocyte globulin conditioning and allogeneic transplantation for patients with myelodysplastic syndromes and myeloproliferative neoplasms.

    Science.gov (United States)

    Benjamin, Jonathan; Chhabra, Saurabh; Kohrt, Holbrook E; Lavori, Philip; Laport, Ginna G; Arai, Sally; Johnston, Laura; Miklos, David B; Shizuru, Judith A; Weng, Wen-Kai; Negrin, Robert S; Lowsky, Robert

    2014-06-01

    Allogeneic hematopoietic cell transplantation (allo HCT) is the only curative therapy for the myelodysplastic syndromes (MDS) and myeloproliferative neoplasms (MPN), but treatment toxicity has been a barrier to its more widespread use. The nonmyeloablative regimen of total lymphoid irradiation (TLI) and antithymocyte globulin (ATG) permits the establishment of donor hematopoiesis necessary for the graft-versus-malignancy effect and is protective against acute graft-versus-host disease (aGVHD), but it has minimal direct cytotoxicity against myeloid diseases. We explored the use of TLI-ATG conditioning to treat 61 patients with allo HCT for MDS (n = 32), therapy-related myeloid neoplasms (n = 15), MPN (n = 9), and chronic myelomonocytic leukemia (n = 5). The median age of all patients was 63 years (range, 50 to 73). The cumulative incidence of aGVHD grades II to IV was 14% (95% confidence interval [CI], 4% to 23%) and for grades III to IV, 4% (95% CI, 0 to 9%), and it did not differ between patients who received allografts from related or unrelated donors. The cumulative incidence of nonrelapse mortality (NRM) at 100 days, 12 months, and 36 months was 0%, 7%, and 11%. Overall survival and progression-free survival were 41% (95% CI, 29% to 53%) and 35% (95% CI, 23% to 48%), respectively. The safety and tolerability of TLI-ATG, as exemplified by its low NRM, provides a foundation for further risk-adapted or prophylactic interventions to prevent disease progression. PMID:24607552

  13. Allogeneic hematopoietic cell transplantation after failed autologous transplant for lymphoma using TLI and anti-thymocyte globulin conditioning.

    Science.gov (United States)

    Rezvani, A R; Kanate, A S; Efron, B; Chhabra, S; Kohrt, H E; Shizuru, J A; Laport, G G; Miklos, D B; Benjamin, J E; Johnston, L J; Arai, S; Weng, W-K; Negrin, R S; Strober, S; Lowsky, R

    2015-10-01

    We describe 47 patients with lymphoma and failed prior autologous hematopoietic cell transplantation (HCT) who received TLI-ATG (anti-thymocyte globulin) conditioning followed by allogeneic HCT. Thirty-two patients had non-Hodgkin lymphoma (NHL; diffuse large B-cell lymphoma (n=19), T-cell NHL (n=6), mantle cell lymphoma (n=4) or other B-cell subtypes (n=3)), and 15 had Hodgkin lymphoma. The median follow-up was 4.9 (range, 2.1-11.9) years. The cumulative incidence of grade II-IV acute GvHD at day +100 was 12%, and the cumulative incidence of extensive chronic GvHD at 1 year was 36%. The 3-year cumulative incidences of overall survival (OS), PFS and non-relapse mortality (NRM) were 81%, 44% and 7%, respectively. Fifteen patients died (relapse, n=10; NRM, n=5). Among the 25 patients with relapse after allogeneic HCT, 11 (44%) achieved durable (>1 year) CRs following donor lymphocyte infusion or chemoradiotherapy. The majority of surviving patients (75%; n=24) were able to discontinue all immunosuppression. For patients with relapsed lymphoma after autologous HCT, allogeneic HCT using TLI-ATG conditioning is a well-tolerated, predominantly outpatient therapy with low NRM (7% at 3 years), a low incidence of GvHD, durable disease control and excellent OS (81% at 3 years). PMID:26146806

  14. Measurement for serum thyroxine-binding globulin (TBG) and its clinical assessment in diagnosis of thyroid states

    International Nuclear Information System (INIS)

    Serum levels of thyroxine (T4)-binding globulin (TBG) were determined by a radioimmunoassay using cellulose-linked antibody to TBG. Values obtained in healthy young adults averaged 1.62 +- 0.25 (SD) mg/100 ml, and no significant difference was detected between males and females. The TBG levels remained within the normal limit in hyperthyroidism while they were significantly increased in hypothyroidism. Interestingly enough, TBG levels were significantly elevated in chronic thyroidities with no overt hypothyroidism. In normal pregnancy, TBG was increased slightly in the first trimester, and markedly in the second and third trimesters. In one case of congenital TBG deficiency, no immunoreactive TBG was detected. It was demonstrated, further, that an inverse relationship (r = -0.7593) existed between the TBG level and serum triiodothyronine uptake index, and that a direct relation (r = +0.6557) was present between the TBG level and T4 in sera from normal subjects and pregnancy. Ratios of T4/TBG were markedly increased in hyperthyroidism, and decreased in hypothyroidism, showing no overlap with the normal subjects, whereas they were below the normal limit in half the cases in the second and third trimesters of pregnancy. The radioimmunoassay for TBG was useful in evaluating hypothyroid states, because it could differentiate the increase in T4 associated with elevated TBG from hyperthyroidism. (author)

  15. A review of human anti-globulin antibody (HAGA, HAMA, HACA, HAHA) responses to monoclonal antibodies. Not four letter words

    Energy Technology Data Exchange (ETDEWEB)

    Mirick, G. R.; Bradt, B. M.; Denardo, S. J.; Denardo, G. L. [Calfornia Univ., Sacramento (United States). Davis Medical Center

    2004-12-01

    The United States Food and Drugs Administration (FDA) has approved unconjugated monoclonal antibodies (MAbs) for immunotherapy (IT) of B-cell lymphoma, breast cancer and acute myeloid leukemia. More recently, approval has been given for conjugated ZevalinTM ({sup 9}0yttrium ibritumomab tiuxetan, IDEC-Y2B8, Biogen Idec, Cambridge, MA) and BexxarTM ({sup 1}31I-tositumomab, Corixa, Corp., Seattle, WA and GlaxoSmithKline, Philadelphia, PA) antiCD20 MAns for use in radioimmunotherapy (RIT) of non-Hodgikin's lymphoma (NHL), thus redefining the standard care of cancer patients. Because of, and despite a lack of basis for concern about allergic reactions due to human antibody responses to these foreign proteins, essays were developed to determine HAGE (human anti-globulin antibody) levels that developed in patient sera following treatment with MAbs. Strategies were also devised to humanize MAbs and to temporarily block patient immune function with drugs in order to decrease the seroconversion rates, with considerable success. On the other hand, a survival advantage has been observed in some patients who developed a HAGA following treatment. This correlates with development of an anti-idiotype antibody cascade directed toward the MAbs used to treat these patients. What follows is a selective review of HAGA and its effect on cancer treatment over the past 2 decades.

  16. Biological half-life and transfer of maternal corticosteroid-binding globulin to amniotic fluid in the rabbit

    International Nuclear Information System (INIS)

    Rabbit corticosteroid-binding globulin (CBG) from the serum of pregnant and nonpregnant females differs in terms of charge microheterogeneity, and both forms were, therefore, radiolabeled and injected iv into 23- to 27-day pregnant rabbits (n = 6) to assess their biological half-lives and possible transfer to the fetal compartment. After an initially rapid distribution phase, the serum half-lives of both forms of [125I]CBG were essentially identical (approximately 13 h) and did not vary at different gestational ages. There was also no difference in the transfer of either form of [125I]CBG from maternal to fetal compartments in any of the animals studied. Moreover, [125I]CBG showed no sign of degradation and retained its steroid-binding activity in fetal urine and amniotic fluid. Twenty-two hours after administration of [125I]CBG to rabbits (n = 2) at 23 days gestation, its mean level in fetal urine (7 cpm/microliter) and amniotic fluid (2.5 cpm/microliter) was much higher than that in fetal blood (0.6 cpm/microliter). More importantly, the specific activities of [125I]CBG in fetal urine and amniotic fluid were comparable to that in maternal serum, and approximately 2 orders of magnitude higher than that in fetal serum. Taken together, these results suggest that CBG in fetal urine and amniotic fluid is largely of maternal origin, and that maternal CBG crosses the fetal kidney preferentially

  17. Electroimmunoassay of sex hormone binding globulin. Enhanced sensitivity by autoradiography using A-ring 125I-17-?-oestradiol

    International Nuclear Information System (INIS)

    Four different 125I-iodinated steroids were tested for their binding to human sex hormone binding globulin (SHBG) using an electrophoretic technique. 17-?-oestradiol iodinated in its A-ring bound with high affinity to SHBG. This radioactive steroid was used to increase the sensitivity of the electroimmunoassay of SHBG by adding the steroid to the samples before electroimmunoassay. The radioactive steroid incorporated into the immunoprecipitates could be observed by autoradiography. The sensitivity of the assay, which employed a rabbit antiserum against purified human SHBG and was standardized with pure SHBG, was about 0.2 mg/1. The coefficient of variation within and between assays was 2.4% and 2.6% respectively, for values within the normal range. The mean SHBG concentration in healthy regularly menstruating women was 3.50 +- 0.74 (SD) mg/1 when measured in plasma, and 3.78 +- 0.80 mg/1 when measured in serum. The corresponding mean concentrations in healthy men were 2.26 +- 0.45 and 2.44 +- 0.49 mg/1. The modified electroimmunoassay described is a simple modification, which increases the sensitivity sufficiently to permit reliable quantification of SHBG over the entire range of concentration which could be relevant in clinical practice. (author)

  18. The effect of cyclic and continuous estrogen replacement therapy on sex hormone binding globulins and androgen levels

    Directory of Open Access Journals (Sweden)

    Ercan M AYGEN

    2003-03-01

    Full Text Available Objective: The serum levels of sex hormone binding globulin (SHBG, free testosteron (fT and dehidroepiandrostenedione sulphate (DHEASO4 which differ following menopause, are effected by estrogens. Therefore oral conjugated equine estrogen (CEE were administered by two different regimens and the consequent hormone levels measured.Materials and Methods: Twenty five postmenopausal women were divided into two groups. Thirteen women received 0.625mg/day CEE (premarin,Wyeth continously and 5 mg/day medroxyprogesterone acetate (MPA (Farlutal, Deva between 14-25 days (group I. Twelve patients received 0.625 mg/day CEE between 1- 21 days and 5 mg/day MPA between 14-25 days (groupII. Both regimens were given to the patients for 6 months.Results: While levels of SHBG significantly raised, the fT and DHEASO4 levels significantly decreased within the two groups. However these hormone levels did not differ between the groups.Conclusion: Both regimens affected SHBG, fT and DHEASO4 levels. But the difference did not show significance between the groups. We conclude that studies in larger groups with a longer treatment period would be more enlightening to determine the difference between the groups.

  19. A family of antimicrobial peptides is produced by processing of a 7S globulin protein in Macadamia integrifolia kernels.

    Science.gov (United States)

    Marcus, J P; Green, J L; Goulter, K C; Manners, J M

    1999-09-01

    A new family of antimicrobial peptides has been discovered in Macadamia integrifolia. The first member of this new family to be purified from nut kernels was a peptide of 45 aa residues, termed MiAMP2c. This peptide inhibited various plant pathogenic fungi in vitro. cDNA clones corresponding to MiAMP2c encoded a 666 aa precursor protein homologous to vicilin 7S globulin proteins. The deduced precursor protein sequence contained a putative hydrophobic N-terminal signal sequence (28 aa), an extremely hydrophilic N-proximal region (212 aa), and a C-terminal region of 426 aa which is represented in all vicilins. The hydrophilic portion of the deduced protein contained the sequence for MiAMP2c as well as three additional segments having the same cysteine spacing pattern as MiAMP2c. Each member of the MiAMP2 family (i.e. MiAMP2a, b, c and d) consisted of approximately 50 amino acids and contained a C-X-X-X-C-(10-12)X-C-X-X-X-C motif. Subsequent isolations from seed exudates led to the purification of the predicted family members MiAMP2b and 2d, both of which also exhibited antimicrobial activity in vitro. These results suggest that some vicilins play a role in defence during seed germination. PMID:10571855

  20. A retrospective comparison of cyclophosphamide plus antithymocyte globulin with cyclophosphamide plus busulfan as the conditioning regimen for severe aplastic anemia

    Directory of Open Access Journals (Sweden)

    L.V.M. Ommati

    2009-03-01

    Full Text Available Allogeneic hematopoietic stem cell transplantation (AHSCT is the treatment of choice for young patients with severe aplastic anemia (SAA. The association of antithymocyte globulin (ATG and cyclophosphamide (CY is the most frequently used conditioning regimen for this disease. We performed this retrospective study in order to compare the outcomes of HLA-matched sibling donor AHSCT in 41 patients with SAA receiving cyclophosphamide plus ATG (ATG-CY, N = 17 or cyclophosphamide plus busulfan (BU-CY, N = 24. The substitution of BU for ATG was motivated by the high cost of ATG. There were no differences in the clinical features between the two groups, including age, gender, cytomegalovirus status, ABO match, interval between diagnosis and transplant, and number of total nucleated cells infused. No differences were observed in the time to neutrophil and platelet engraftment, or in the risk of veno-occlusive disease and hemorrhage. However, there was a higher risk of mucositis in the BU-CY group (71 vs 24%, P = 0.004. There were no differences in the incidence of neutrophil and platelet engraftment, acute and chronic graft-versus-host disease, and transplant-related mortality. There was a higher incidence of late rejection in the ATG-CY group (41 vs 4%, P = 0.009. Although the ATG-CY group had a longer follow-up (101 months than the BU-CY group (67 months, P = 0.04, overall survival was similar between the groups (69 vs 58%, respectively, P = 0.32. We conclude that the association BU-CY is a feasible option to the conventional ATG-CY regimen in this population.

  1. A retrospective comparison of cyclophosphamide plus antithymocyte globulin with cyclophosphamide plus busulfan as the conditioning regimen for severe aplastic anemia

    Scientific Electronic Library Online (English)

    L.V.M., Ommati; C.A., Rodrigues; A.R., Silva; L.P., Silva; M.L.L.F., Chaufaille; J.S.R., Oliveira.

    2009-03-01

    Full Text Available Allogeneic hematopoietic stem cell transplantation (AHSCT) is the treatment of choice for young patients with severe aplastic anemia (SAA). The association of antithymocyte globulin (ATG) and cyclophosphamide (CY) is the most frequently used conditioning regimen for this disease. We performed this r [...] etrospective study in order to compare the outcomes of HLA-matched sibling donor AHSCT in 41 patients with SAA receiving cyclophosphamide plus ATG (ATG-CY, N = 17) or cyclophosphamide plus busulfan (BU-CY, N = 24). The substitution of BU for ATG was motivated by the high cost of ATG. There were no differences in the clinical features between the two groups, including age, gender, cytomegalovirus status, ABO match, interval between diagnosis and transplant, and number of total nucleated cells infused. No differences were observed in the time to neutrophil and platelet engraftment, or in the risk of veno-occlusive disease and hemorrhage. However, there was a higher risk of mucositis in the BU-CY group (71 vs 24%, P = 0.004). There were no differences in the incidence of neutrophil and platelet engraftment, acute and chronic graft-versus-host disease, and transplant-related mortality. There was a higher incidence of late rejection in the ATG-CY group (41 vs 4%, P = 0.009). Although the ATG-CY group had a longer follow-up (101 months) than the BU-CY group (67 months, P = 0.04), overall survival was similar between the groups (69 vs 58%, respectively, P = 0.32). We conclude that the association BU-CY is a feasible option to the conventional ATG-CY regimen in this population.

  2. Somatostatin Is Essential for the Sexual Dimorphism of GH Secretion, Corticosteroid-Binding Globulin Production, and Corticosterone Levels in Mice

    Science.gov (United States)

    Adams, Jessica M.; Otero-Corchon, Veronica; Hammond, Geoffrey L.; Veldhuis, Johannes D.; Qi, Nathan

    2015-01-01

    Distinct male and female patterns of pituitary GH secretion produce sexually differentiated hepatic gene expression profiles, thereby influencing steroid and xenobiotic metabolism. We used a fully automated system to obtain serial nocturnal blood samples every 15 minutes from cannulated wild-type (WT) and somatostatin knockout (Sst-KO) mice to determine the role of SST, the principal inhibitor of GH release, in the generation of sexually dimorphic GH pulsatility. WT males had lower mean and median GH values, less random GH secretory bursts, and longer trough periods between GH pulses than WT females. Each of these parameters was feminized in male Sst-KO mice, whereas female Sst-KO mice had higher GH levels than all other groups, but GH pulsatility was unaffected. We next performed hepatic mRNA profiling with high-density microarrays. Male Sst-KO mice exhibited a globally feminized pattern of GH-dependent mRNA levels, but female Sst-KO mice were largely unaffected. Among the differentially expressed female-predominant genes was Serpina6, which encodes corticosteroid-binding globulin (CBG). Increased CBG was associated with elevated diurnal peak plasma corticosterone in unstressed WT females and both sexes of Sst-KO mice compared with WT males. Sst-KO mice also had exaggerated ACTH and corticosterone responses to acute restraint stress. However, consistent with their lack of phenotypic signs of excess glucocorticoids, cerebrospinal fluid concentrations of free corticosterone in Sst-KO mice were not elevated. In summary, SST is necessary for the prolonged interpulse troughs that define masculinized pituitary GH secretion. SST also contributes to sexual dimorphism of the hypothalamic-pituitary-adrenal axis via GH-dependent regulation of hepatic CBG production. PMID:25551181

  3. A rapid release of corticosteroid-binding globulin from the liver restrains the glucocorticoid hormone response to acute stress.

    Science.gov (United States)

    Qian, Xiaoxiao; Droste, Susanne K; Gutièrrez-Mecinas, María; Collins, Andrew; Kersanté, Flavie; Reul, Johannes M H M; Linthorst, Astrid C E

    2011-10-01

    A strict control of glucocorticoid hormone responses to stress is essential for health. In blood, glucocorticoid hormones are for the largest part bound to corticosteroid-binding globulin (CBG), and just a minor fraction of hormone is free. Only free glucocorticoid hormone is able to exert biological effects, but little is known about its regulation during stress. We found, using a dual-probe in vivo microdialysis method, that in rats, the forced-swim stress-induced rise in free corticosterone (its major glucocorticoid hormone) is strikingly similar in the blood and in target compartments such as the subcutaneous tissue and the brain. However, in all compartments, the free corticosterone response was delayed by 20-30 min as compared with the total corticosterone response in the blood. We discovered that CBG is the key player in this delay. Swim stress evoked a fast (within 5 min) and profound rise in CBG protein and binding capacity in the blood through a release of the protein from the liver. Thus, the increase in circulating CBG levels after stress restrains the rise in free corticosterone concentrations for approximately 20 min in the face of mounting total hormone levels in the circulation. The stress-induced increase in CBG seems to be specific for moderate and strong stressors. Both restraint stress and forced swimming caused an increase in circulating CBG, whereas its levels were not affected by mild novelty stress. Our data uncover a new, highly dynamic role for CBG in the regulation of glucocorticoid hormone physiology after acute stress. PMID:21828178

  4. Corticosteroid-binding globulin contributes to the neuroendocrine phenotype of mice selected for extremes in stress reactivity.

    Science.gov (United States)

    Mattos, Gabriele E; Heinzmann, Jan-Michael; Norkowski, Stefanie; Helbling, Jean-Christophe; Minni, Amandine M; Moisan, Marie-Pierre; Touma, Chadi

    2013-12-01

    Increasing evidence indicates an important role of steroid-binding proteins in endocrine functions, including hypothalamic-pituitary-adrenal (HPA) axis activity and regulation, as they influence bioavailability, local delivery, and cellular signal transduction of steroid hormones. In the plasma, glucocorticoids (GCs) are mainly bound to the corticosteroid-binding globulin (CBG) and to a lesser extend to albumin. Plasma CBG levels are therefore involved in the adaptive stress response, as they determine the concentration of free, biologically active GCs. In this study, we investigated whether male mice with a genetic predisposition for high-reactivity (HR), intermediate-reactivity (IR), or low-reactivity (LR) stress-induced corticosterone (CORT) secretion present different levels of free CORT and CORT-binding proteins, basally and in response to stressors of different intensity. Our results suggest a fine control interaction between plasma CBG expression and stress-induced CORT release. Although plasma CBG levels, and therefore CBG binding capacity, were higher in HR animals, CORT secretion overloaded the CBG buffering function in response to stressors, resulting in clearly higher free CORT levels in HR compared with IR and LR mice (HR>IR>LR), resembling the pattern of total CORT increase in all three lines. Both stressors, restraint or forced swimming, did not evoke fast CBG release from the liver into the bloodstream and therefore CBG binding capacity was not altered in our three mouse lines. Thus, we confirm CBG functions in maintaining a dynamic equilibrium between CBG-bound and unbound CORT, but could not verify its role in delaying the rise of plasma free CORT immediately after stress exposure. PMID:24048966

  5. The binding properties of pyrethroids to human skin fibroblast androgen receptors and to sex hormone binding globulin.

    Science.gov (United States)

    Eil, C; Nisula, B C

    1990-03-01

    The pyrethroids are a class of natural and synthetic pesticides which were associated with an epidemic of gynecomastia in Haitian men in 1981. In the present study we tested several pyrethroids for their ability to interact with androgen binding sites in dispersed, intact human genital skin fibroblasts and in human plasma to sex hormone binding globulin (SHBG). All the pyrethroids tested inhibited fibroblast binding of [3H]methyltrienolone (R1881) at 22 degrees C with the following rank order of potency:pyrethrins greater than bioallethrin greater than fenvalerate greater than fenothrin greater than fluvalinate greater than permethrin greater than resmethrin. 50% displacement of [3H]R1881 binding to fibroblast androgen receptors was achieved by 1.5-44 x 10(-5) M concentrations of the competitors, respectively. Previous studies with cimetidine, a known inhibitor of androgen receptor binding, showed 50% competition at a concentration of 1.4 x 10(-4) M in this system. Scatchard analysis of binding experiments performed with increasing concentrations of [3H]R1881 in the presence of the pyrethroids indicated that the binding inhibition was competitive. On the other hand, of the pyrethroids examined only the pyrethrins (50% inhibition) and bioallethrin (43% inhibition) were able to displace [3H]testosterone from SHBG when tested at a concentration of 10(-4) M. These data indicate that a novel class of non-steroidal compounds, the pyrethroids, can interact competitively with human androgen receptors and SHBG. These findings provide a mechanism by which chronic exposure of humans or animals to pesticides containing these compounds may result in disturbances in endocrine effects relating to androgen action. PMID:2325407

  6. Regulation of IgM anti-bovine ?-globulin antibody formation by helper and suppressor T cells in rabbits

    International Nuclear Information System (INIS)

    Primary IgM anti-bovine ?-globulin (BGG) antibody formation was studied in rabbits subjected to 500 or 900 R of whole body X-irradiation in combination with one or more of the following conditions: appendectomy (Ax), thymectomy (Tx), shielding of appendix (As) or bone marrow (Bs) during irradiation (X), transfer of 109 appendix or thymus cells (Ac or Tc), and immediate immunization with 200 ?g of alum-precipitated BGG iv. The IgM antibody response was well preserved in AsX rabbits in comparison with X500 rabbits. Conversely, the response was suppressed in AxX500 rabbits although transfer of autologous appendix cells to these animals (AxX500Ac) restored the response. In the group receiving 900 R, there was variable restoration in individual AxX.Ac and AxBsX.Ac rabbits and little or none in AxTxX.Tc, AxTxBsX.Tc, and AxTxBsX.Ac animals. However, simultaneous injections of autologous thymus cells and appendix lymphocytes (AxTxX.Ac.Tc) restored the response completely. The IgM response was somewhat enhanced in AsTxX animals and returned again to the level of normal controls by injecting these animals with autologous thymus cells. However, marked suppression was seen when AsTxX rabbits were injected with neonatal allogeneic thymus cells. A remarkable enhancement of IgM antibody formation was induced in normal rabbits by injection of PHA into the appendiceal artery. On the basis of these results and of histological observations, it was suggested that the rabbit appendix represents a large pool of both B and T cells and that appendix-derived B cells participating in IgM anti-BGG antibody formation are under the regulation of both helper and suppressor T cells

  7. Preparation of 125I-labeled human thyroxine-binding alpha globulin and its turnover in normal and hypothyroid subjects

    International Nuclear Information System (INIS)

    A protein with the electrophoretic, immunologic, and hormone-binding properties of thyroxine-binding globulin (TBG) has been prepared from human plasma and labeled with radioiodine (125I) by an enzymatic method of iodination. The [125I]TBG retained the electrophoretic and immunologic characteristics of unlabeled TBG but exhibited a partial loss of thyroxine-binding activity, as assessed by affinity chromatography. The in vivo behavior of [125I]TBG was studied in six euthyroid subjects (controls) with normal serum levels of TBG as measured both by radioimmunoassay and by determination of maximal T4-binding capacity and in four male patients with untreated primary hyperthyroidism, three of whom had elevated serum TBG. The half-time of the final slope of the plasma disappearance curve averaged 5.0 days +- 1.2 (SD) in the controls and ranged from 3.9 to 10.9 days in the hypothyroid patients. The distribution volume was similar in the two groups, 6.7 +- 1.3 vs. 7.1 +- 2.1 liters. The catabolic clearance rate averaged 0.99 +- 0.33 liters plasma/24 h in the controls and 0.92 +- 0.46 in the hypothyroids. The absolute turnover rate of TBG, calculated from the catabolic clearance rate multiplied by the serum concentration of radioimmunoassayable TBG, averaged 17.8 +- 2.1 mg/day in the controls and ranged from 14.8 to 33.2 mg/day in the hypothyroids. Among the entire group of subjects there was no correlation between the serum TBG concentration and the absolute turnover rate of TBG

  8. Long-term outcome of 25 children and adolescents with severe aplastic anemia treated with antithymocyte globulin

    Directory of Open Access Journals (Sweden)

    de-Medeiros C.R.

    2000-01-01

    Full Text Available Severe aplastic anemia (SAA is probably an immune-mediated disorder, and immunosuppressive therapy is recommended for patients with no available donor for bone marrow transplant. Between October 1984 and November 1987, 25 consecutive children and adolescents with SAA with no HLA-compatible marrow donor received equine antithymocyte globulin (ATG (15 mg kg-1 day-1 for 10 days. The patients were evaluated 6 weeks, 6 months, and 12 months after starting ATG treatment. Thereafter, patients were evaluated yearly until July 1998. Median age was 10 years (range, 1.5-20 years, granulocyte counts on referral ranged from 0.032 to 1.4 x 10(9/l (median 0.256 x 10(9/l, and 12 patients had granulocyte counts <0.2 x 10(9/l. At a median follow-up of 9.6 years (range, 8.6-11.8 years, 10 patients (40% remained alive with good marrow function. No morphologic evidence of hematological clonal disorders has been observed, although two patients probably have acquired clonal chromosomal abnormalities (trisomy 8 and del(6q21, respectively. Responses to ATG were observed between 6 weeks and 6 months from the start of treatment in 60% of evaluable patients. The response rate was not different in patients whose granulocyte count at diagnosis was <0.2 x 10(9/l, or in those who were <10 years of age. This study supports the view that, when compared with supportive measures, ATG is an effective treatment for children or adolescents with SAA. Although these results are inferior to those reported for marrow transplantation or more intensive immunosuppressive regimens, these patients who responded to ATG are long-term survivors with stable peripheral blood counts and a low rate of relapse.

  9. Prediagnostic plasma testosterone, sex hormone binding globulin, IGF-I and hepatocellular carcinoma : Etiological factors or risk markers?

    DEFF Research Database (Denmark)

    Lukanova, Annekatrin; Becker, Susen

    2014-01-01

    Elevated pre-diagnostic testosterone and insulin-like growth factor-I (IGF-I) concentrations have been proposed to increase risk of hepatocellular carcinoma (HCC). However, the metabolism of these hormones is altered as a consequence of liver damage and they may have clinical utility as HCC risk markers. A case-control study was nested within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort and included 125 incident HCC cases and 247 individually matched controls. Testosterone, sex hormone binding globulin (SHBG) and IGF-I were analyzed by immunoassays. Odds ratios (OR) and 95% confidence intervals (CI) were estimated by conditional logistic regression. The area under the receiver operating curves (AUC) was calculated to assess HCC predictive ability of the tested models. After adjustments for epidemiological variables (body mass index, smoking, ethanol intake, hepatitis and diabetes) and liver damage (a score based on albumin, bilirubin, aspartate aminotransaminase, alanine aminotransaminase, gamma-glutamyltransferase and alkaline phosphatase concentrations), only SHBG remained significantly associated with risk (OR for top versus bottom tertile of 3.86 (1.32-11.3), ptrend =0.009). As a single factor SHBG had an AUC of 0.81 (0.75-0.86). A small, but significant increase in AUC was observed when SHBG was added to a model including the liver damage score and epidemiological variables (from 0.89 to 0.91, p=0.02) and a net reclassification of 0.47% (0.45-0.48). The observed associations of HCC with pre-diagnostic SHBG, free testosterone and IGF-I concentrations are in directions opposite to that expected under the etiological hypotheses. SHBG has a potential to be tested as pre-diagnostic risk marker for HCC. © 2013 Wiley Periodicals, Inc.

  10. Induction by anti-thymocyte globulins in kidney transplantation: a review of the literature and current usage

    Science.gov (United States)

    Malvezzi, Paolo; Jouve, Thomas; Rostaing, Lionel

    2015-01-01

    Context: Preventing acute rejection (AR) after kidney transplantation is of utmost importance because an AR can have a negative impact on long-term allograft survival. Evidence Acquisition: Directory of Open Access Journals (DOAJ), Google Scholar, PubMed, EBSCO, and Web of Science have been searched. Results: At the moment this can be done by using rabbit anti-thymocyte globulins (rATGs) as an induction therapy. However, because rATGs are associated with some deleterious side-effects, such as the opportunistic infections cytomegalovirus (CMV) and de novo post-transplant cancer, it is very important they are used optimally, i.e., at minimal doses that avoid many side-effects but still retain optimal treatment efficacy. Recent data show that the risk of CMV infection can be minimized using tacrolimus plus everolimus, and not tacrolimus plus mycophenolic acid, as the maintenance immunosuppression. The use of rATG is particularly valuable in; (a) sensitized patients; (b) in recipients from an expanded-criteria donor, thus enabling the introduction of calcineurin inhibitors at reduced doses; and (c) for patients where steroid avoidance is contemplated. However, we also need to consider that rATG may increase the risk of de novo cancer, even though recent data indicate this is unlikely and that any risk can be reduced by using mammalian target of rapamycin (mTOR) inhibitors instead of mycophenolic acid combined with low-dose calcineurin inhibitors. Conclusions: Even though rATGs do not improve long-term kidney-allograft survival, they may help reduce calcineurin-inhibitor dosage during the early post-transplant period and minimize the risk of AR. PMID:26457257

  11. Energy Storage

    Energy Technology Data Exchange (ETDEWEB)

    Mukundan, Rangachary [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2014-09-30

    Energy storage technology is critical if the U.S. is to achieve more than 25% penetration of renewable electrical energy, given the intermittency of wind and solar. Energy density is a critical parameter in the economic viability of any energy storage system with liquid fuels being 10 to 100 times better than batteries. However, the economical conversion of electricity to fuel still presents significant technical challenges. This project addressed these challenges by focusing on a specific approach: efficient processes to convert electricity, water and nitrogen to ammonia. Ammonia has many attributes that make it the ideal energy storage compound. The feed stocks are plentiful, ammonia is easily liquefied and routinely stored in large volumes in cheap containers, and it has exceptional energy density for grid scale electrical energy storage. Ammonia can be oxidized efficiently in fuel cells or advanced Carnot cycle engines yielding water and nitrogen as end products. Because of the high energy density and low reactivity of ammonia, the capital cost for grid storage will be lower than any other storage application. This project developed the theoretical foundations of N2 catalysis on specific catalysts and provided for the first time experimental evidence for activation of Mo 2N based catalysts. Theory also revealed that the N atom adsorbed in the bridging position between two metal atoms is the critical step for catalysis. Simple electrochemical ammonia production reactors were designed and built in this project using two novel electrolyte systems. The first one demonstrated the use of ionic liquid electrolytes at room temperature and the second the use of pyrophosphate based electrolytes at intermediate temperatures (200 – 300 ºC). The mechanism of high proton conduction in the pyrophosphate materials was found to be associated with a polyphosphate second phase contrary to literature claims and ammonia production rates as high as 5X 10-8 mol/s/cm2 were achieved.

  12. Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L

    International Nuclear Information System (INIS)

    High level expression of soluble amaranth 11S proglobulin in Escherichia coli and its purification are described. Crystallization of the recombinant protein and crystal data collection are also presented. 11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to ?80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0–40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 Å resolution and belonged to space group P63, with unit-cell parameters a = b = 97.6, c = 74.8 Å, ? = 120.0°. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress

  13. Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench

    Directory of Open Access Journals (Sweden)

    Milisavljevi? Mira

    2004-01-01

    Full Text Available Using the modified 5’-RACE approach, a fragment containing the 955 bp long 5’- regulatory region of the buckwheat storage globulin gene (FeLEG1 has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory elements possibly involved in tissue specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research, but also as a useful tool for biotechnological application.

  14. Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

    DEFF Research Database (Denmark)

    Dam, Svend Secher; Thaysen-Andersen, Morten

    2013-01-01

    Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (?20%), pauci-mannosidic (?40%), and complex forms (?40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants ?-1,2-xylose and ?-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectincontaining two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2 (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has the potential to serve as a model system for studying the role of seed proteins and their glycosylation in food allergy.

  15. Níveis séricos de globulinas e a intensidade da fibrose hepática em pacientes com esquistossomose mansônica / Serum globulin levels and intensity of hepatic fibrosis in patients with mansonic schistosomiasis

    Scientific Electronic Library Online (English)

    Henrique S. T., Correia; Ana Lucia C., Domingues; Edmundo P. A., Lopes; Clarice N. L., Morais; Camila, Sarteschi; Izolda M. F., Moura.

    2009-09-01

    Full Text Available CONTEXTO: Tem sido descrita correlação entre os níveis séricos de globulinas e o grau de fibrose hepática nas hepatites crônicas, mas não se encontram relatos na esquistossomose mansônica. OBJETIVO: Avaliar os níveis séricos de globulinas e de IgG, e a intensidade da fibrose periportal mensurada pel [...] a ultrassonografia em pacientes com esquistossomose mansônica. MÉTODOS: Entre novembro de 2006 e fevereiro de 2007, foram estudados 41 pacientes que preencheram ficha clínica e realizaram dosagens de IgG por imunoturbidimetria e de globulinas indiretamente pelo método do biureto. A ultrassonografia foi realizada por um único pesquisador, seguindo os protocolos do Cairo e de Niamey. RESULTADOS: A média de idade foi 41 anos, sendo 25 pacientes (61%) do sexo feminino. Dez dos 41 pacientes (24%) apresentaram elevação dos níveis séricos de globulinas e 21 (51%) dos de IgG. Conforme a classificação do Cairo, 21 pacientes apresentaram grau I de fibrose, 18 grau II e 2 grau III, e pela classificação de Niamey 8 apresentavam padrão C, 20 D e 13 E. Aqueles com graus II ou III de fibrose tiveram maiores níveis de IgG do que os de grau I (P = 0,047), assim como aqueles que apresentaram padrões D e E em relação ao C (P = 0,011). Não houve associação entre os níveis de globulinas e o grau ou padrão de fibrose. CONCLUSÃO: Em pacientes com esquistossomose mansônica, observou-se elevação dos níveis séricos de IgG de acordo com a progressão do grau e do padrão de fibrose periportal, mas o mesmo não se observou com os níveis de globulinas. Abstract in english BACKGROUND: A correlation between the levels of serum globulins and the hepatic fibrosis degree in chronic hepatitis was described, but reports in schistosomiasis mansoni have not been found. OBJECTIVE: To evaluate the serum globulins and IgG levels, and periportal fibrosis intensity measured by ult [...] rasound in patients with schistosomiasis mansoni. METHODS: Between November, 2006 and February 2007, 41 patients which were eligible, filled them a questionnaire and had their levels of serum IgG measured by immunoturbidimetry and globulins indirectly measured by the Biuret method. The ultrasound was carried out by a single researcher, according to the Cairo and Niamey protocols. RESULTS: The average age was 41 years old and 25 female patients (61%). Ten patients (24%) from 41 showed serum globulins levels raised and 21 (51%) presented elevated IgG levels. According to the Cairo classification, 21 patients showed grade I of fibrosis, 18 grade II and 2 grade III; and by the Niamey classification 8 showed standard C, 20 D, and 13 E. Those with grade II or III of fibrosis had higher IgG levels than the ones with grade I (P = 0,047), as well as those who showed standards D and E as compared to C (P = 0,011). There was no association between the globulins levels and the intensity of fibrosis. CONCLUSION: In patients with schistosomiasis mansoni, an increase of the IgG serum levels was observed according to the progression from periportal fibrosis intensity, but the same was not founded with globulins levels.

  16. Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance

    DEFF Research Database (Denmark)

    Kroghsbo, Stine; BØgh, Katrine Lindholm

    2011-01-01

    Background: It is not known why some foods sensitizing via the gastrointestinal tract are prevalent allergenic foods and others are not. Eating habits, processing, and the food matrix have been suggested to influence the allergenicity of a given food. Factors related to protein structure, such as stability to digestion, have also been suggested. 7S globulins from peanut, hazelnut, soy, and pea were studied to determine whether related proteins would induce a similar sensitization when removed from their ‘normal’ matrix. Methods: Brown Norway rats (soy tolerant or nontolerant) were immunized i.p. 3 times with 100 ?g purified peanut, hazelnut, soy, or pea 7S without adjuvant. Sera were analyzed for specific antibodies by different ELISAs (IgG1, IgG2a, and IgE), inhibition ELISA, and rat basophilic leukemia cell assay. Results: The 4 related 7S globulins induced a response with an almost identical level of specific antibodies, but peanut 7S induced IgE of higher avidity than hazelnut and pea 7S which, again, hada higher avidity than IgE induced by soy 7S. Soy tolerance reduced the functionality of IgE without influencing antibody titers. Conclusions: Although the 4 7S globulins are structurally related allergens, they induce antibodies with different antigen-binding characteristics. Peanut 7S induces IgE of a higher avidity than hazelnut and pea 7S which, again, has a higher avidity than IgE induced by soy 7S. We also show that soy tolerance influences the function of antibodies to peanut 7S. These findings may help explain how antibodies of different clinical significances can develop in different individuals sensitized to the same allergen.Copyright © 2011 S. Karger AG, Basel

  17. The role of sex hormone binding globulin (SHBG) in conversion of testosterone to 5 ?-dihydrotestosterone and androstenedione in skin from suprapubic area of women. Part 1. In vitro study with skin sections

    International Nuclear Information System (INIS)

    The influence of sex hormone binding globulin (SHBG) on testosterone metabolism into 5 alpha-dihydrotestosterone and androstenedione in skin sections from the suprapubic area women of reproductive age was investigated. The SHBG was obtained by precipitating plasma proteins of women in the third trimester of pregnancy with ammonium sulphate. Steroids were isolated from the incubation medium by extraction with acetone and reextraction with ether and purified using column chromatography on aluminum oxide, thin layer chromatography (TLC) on silica gel and following formation of acetyl derivatives the thin layer chromatography on silica gel was again carried out. Individual recovery of androstenedione was calculated using radioisotope and the recovery of 5 alpha-dihydrotestosterone was determined with gas chromatography. Mean value of identified 5 alpha-dihydrotestosterone in the presence of inactive globulin was 52.77 fg/mg, whereas following the addition of active globulin the value decreased to 19.62 fg/mg. Mean value of identified androstenedione in preparations with inactive globulin (62.97 fg/mg), in the presence of active globulin was also reduced to 18.15 fg/mg. (author)

  18. INDUCTION OF PROTEIN DROPLET (ALPHA(2-MU)-GLOBULIN) NEPHROPATHY IN MALE-RATS AFTER SHORT-TERM DOSAGE WITH 1,8-CINEOLE AND L-LIMONENE

    DEFF Research Database (Denmark)

    Kristiansen, E.; Madsen, Charlotte Bernhard

    1995-01-01

    As part of a series of short-term studies on peppermint oil constituents for their possible induction of the encephalopathy found with peppermint oil, 1,8-cineole and l-limonene were studied. Groups of 10 male Wistar rats were given 0, 500, or 1000 mg 1,8-cineole/kg body wt./day or 0, 800, or 1600 mg l-limonene/kg body wt./day for 2& days. 1,8-Cineole and l-limonene both induced accumulation of protein droplets containing alpha(2 mu)-globulin in proximal tubular epithelial cells in male rats. Th...

  19. Dietary ?-Tocopherol and Linoleic Acid, Serum Insulin, and Waist Circumference Predict Circulating Sex Hormone-Binding Globulin in Premenopausal Women1–4

    OpenAIRE

    Nayeem, Fatima; Nagamani, Manubai; Anderson, Karl E; Huang, Yafei; Grady, James J; Lu, Lee-Jane W.

    2009-01-01

    Reduced levels of circulating sex hormone-binding globulin (SHBG) are implicated in the etiology of sex steroid-related pathologies and the metabolic syndrome. Dietary correlates of serum SHBG remain unclear and were studied in a convenient cross-sectional sample of healthy 30- to 40-y-old women (n = 255). By univariate analyses, serum SHBG correlated negatively with several indices of the metabolic syndrome, such as BMI, waist circumference, hip circumference (r = ?0.36 to ?0.44; P < 0.0001)...

  20. Anti-thymocyte globulin treatment of marrow aplasia associated with paroxysmal nocturnal haemoglobinuria (PNH) resulted in haematological improvement due to an expansion of the PNH clone.

    Science.gov (United States)

    Cheung, W C; Lam, C C K; Kwong, Y L

    2003-01-01

    A patient with aplastic anaemia developed paroxysmal nocturnal haemoglobinuria (PNH) 4 years after diagnosis, with an ensuing haematopoietic improvement. The PNH clone subsequently declined, leading to pancytopenia again. Anti-thymocyte globulin had to be administered 14 years later, which resulted in haematopoietic improvement once more. Flow cytometric analysis showed that this was attributable to expansion of the PNH clone, owing probably to alleviation of its suppression by immune-mediated mechanisms. PIG-A gene analysis showed that the same PNH clone had waned and waxed in the clinical course. Our results suggest that the PNH clone might rarely be an immune target as well. PMID:12542494

  1. Langerhans cell histiocytosis in an adult with involvement of the calvarium, cerebral cortex and brainstem: discussion of pathophysiology and rationale for the use of intravenous immune globulin.

    Science.gov (United States)

    Dardis, Christopher; Aung, Thandar; Shapiro, William; Fortune, John; Coons, Stephen

    2015-01-01

    We report a case of Langerhans cell histiocytosis in a 64-year-old male who presented with symptoms and signs of brain involvement, including seizures and hypopituitarism. The diagnosis was confirmed with a biopsy of a lytic skull lesion. The disease affecting the bone showed no sign of progression following a short course of cladribine. Signs of temporal lobe involvement led to an additional biopsy, which showed signs of nonspecific neurodegeneration and which triggered status epilepticus. Lesions noted in the brainstem were typical for the paraneoplastic inflammation reported in this condition. These lesions improved after treatment with cladribine. They remained stable while on treatment with intravenous immune globulin. PMID:25873887

  2. Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma

    International Nuclear Information System (INIS)

    This report describes investigations into the use of sodium salicylate as a cortisol-binding-globulin blocking agent and the subsequent development of a radioimmunoassay for cortisol on unextracted plasma. Cortisol antiserum was raised against a cortisol 3-0-(carboxy-methyl) oxime-bovine serum albumin conjugate. A 125I-labelled cortisol-tyrosine methyl ester conjugate was also prepared for use in the assay. The radioimmunoassay developed involved no pre-treatment or extraction of the samples before analysis and was extremely simple to perform. Comparison with another radioimmunoassay for cortisol and with the Mattingly fluorimetric assay gave good correlation. (author)

  3. Identification, characterization and expression of novel Sex Hormone Binding Globulin alternative first exons in the human prostate

    Directory of Open Access Journals (Sweden)

    de Torres Inés

    2009-06-01

    Full Text Available Abstract Background The human Sex Hormone Binding Globulin (SHBG gene, located at 17p13.1, comprises, at least, two different transcription units regulated by two different promoters. The first transcription unit begins with the exon 1 sequence and is responsible for the production of plasma SHBG by the hepatocytes, while the second begins with an alternative exon 1 sequence, which replaces the exon 1 present in liver transcripts. Alternative exon 1 transcription and translation has only been demonstrated in the testis of transgenic mice containing an 11-kb human SHBG transgene and in the human testis. Our goal has been to further characterize the 5' end of the SHBG gene and analyze the presence of the SHBG alternative transcripts in human prostate tissue and derived cell lines. Results Using a combination of in silico and in vitro studies, we have demonstrated that the SHBG gene, along with exon 1 and alternative exon 1 (renamed here exon 1A, contains four additional alternative first exons: the novel exons 1B, 1C, and 1E, and a previously identified exon 1N, which has been further characterized and renamed as exon 1D. We have shown that these four alternative first exons are all spliced to the same 3' splice site of SHBG exon 2, and that exon 1A and the novel exon 1B can be spliced to exon 1. We have also demonstrated the presence of SHBG transcripts beginning with exons 1B, 1C and 1D in prostate tissues and cell lines, as well as in several non-prostatic cell lines. Finally, the alignment of the SHBG mammalian sequences revealed that, while exons 1C, 1D and 1E are very well conserved phylogenetically through non-primate mammal species, exon 1B probably aroused in apes due to a single nucleotide change that generated a new 5' splice site in exon 1B. Conclusion The identification of multiple transcription start sites (TSS upstream of the annotated first exon of human SHBG, and the detection of the alternative transcripts in human prostate, concur with the prediction of the ENCODE (ENCyclopedia of DNA Elements project, and suggest that the regulation of SHBG is much more complex than previously reported.

  4. Storage pond

    International Nuclear Information System (INIS)

    A pond is described for the storage of hazardous materials, such as irradiated nuclear fuel elements, under water. Upper and lower impervious membranes extend without interruption beneath the floor of the pond and the edges of the membranes lead into a trench surrounding the pond. Any leakage through the floor is directed normally by the upper membrane into the trench. The lower membrane provides an additional impervious barrier in the event of a leak in the upper membrane and again directs the leakage into the trench thereby avoiding contamination of the ground beneath the pond. (author)

  5. Battery storage

    Scientific Electronic Library Online (English)

    César A.C., Sequeira; Mário R., Pedro.

    2008-12-01

    Full Text Available As baterias alcalinas, as de matérias activas exteriores, as de sódio e as de lítio constituem uma forma excelente para o armazenamento de energia química e podemos encará-las como muito promissoras para aplicações de armazenamento de energia em larga escala. Este artigo inclui alguns comentários pe [...] rtinentes sobre estes sistemas recarregáveis, na sua actual fase de investigação e desenvolvimento. Abstract in english This paper focuses on four generic types of secondary battery - Alkaline batteries, Flow batteries, Sodium batteries, and Lithium batteries - that hold most promise for the storage of energy. [...

  6. Hybrid Storage

    Science.gov (United States)

    Micheloni, R.; Crippa, L.; Picca, M.

    In recent years, both industry and academia have increased their research effort in the hybrid memory management space, developing a wide variety of systems. It is worth mentioning that "hybrid" is a generic term and it can have different meanings depending on the context. For instance, a storage system can be hybrid because it combines HDD and SSD; an SSD can be hybrid because it combines SLC and MLC Flash memories, or it combines different non-volatile memories like NAND and ReRAM. In this chapter we look at all these different meanings.

  7. Dossier: underground storage

    International Nuclear Information System (INIS)

    This dossier reviews the main concepts of storage in geologic formations: shape of artificial cavities; natural reservoirs: natural gas storage in aquifers, heat storage, karsts and caves; artificial reservoirs: salt dissolution cavities, salt mines, enlargement of cavities, storage of metal wastes; reservoirs in mining cavities: hydrocarbons storage (tightness, steel coated cavities), cryogenic storage; use of ancient infrastructures (mines, quarries, galleries): hydrocarbons storage, toxic wastes storage, radioactive wastes disposal, reversible radioactive wastes storage, solar neutrons trapping in underground galleries, storage of film archives etc.. (J.S.)

  8. Gas storage materials, including hydrogen storage materials

    Science.gov (United States)

    Mohtadi, Rana F; Wicks, George G; Heung, Leung K; Nakamura, Kenji

    2014-11-25

    A material for the storage and release of gases comprises a plurality of hollow elements, each hollow element comprising a porous wall enclosing an interior cavity, the interior cavity including structures of a solid-state storage material. In particular examples, the storage material is a hydrogen storage material, such as a solid state hydride. An improved method for forming such materials includes the solution diffusion of a storage material solution through a porous wall of a hollow element into an interior cavity.

  9. Gas storage materials, including hydrogen storage materials

    Science.gov (United States)

    Mohtadi, Rana F; Wicks, George G; Heung, Leung K; Nakamura, Kenji

    2013-02-19

    A material for the storage and release of gases comprises a plurality of hollow elements, each hollow element comprising a porous wall enclosing an interior cavity, the interior cavity including structures of a solid-state storage material. In particular examples, the storage material is a hydrogen storage material such as a solid state hydride. An improved method for forming such materials includes the solution diffusion of a storage material solution through a porous wall of a hollow element into an interior cavity.

  10. Biomarker for Glycogen Storage Diseases

    Science.gov (United States)

    2015-06-12

    Fructose Metabolism, Inborn Errors; Glycogen Storage Disease; Glycogen Storage Disease Type I; Glycogen Storage Disease Type II; Glycogen Storage Disease Type III; Glycogen Storage Disease Type IV; Glycogen Storage Disease Type V; Glycogen Storage Disease Type VI; Glycogen Storage Disease Type VII; Glycogen Storage Disease Type VIII

  11. Association of the pituitary-testicular axis function and sex hormone-binding globulin with melatonin secretion in morbidly obese men

    International Nuclear Information System (INIS)

    A possible relationship between melatonin (MEL) secretion and pituitary-testicular function as well as the circadian rhythmicity of serum MEL, lutropin (LH), folitropin (FSH), estradiol (E2), total testosterone (T) and sex hormone-binding globulin (SHBG) were evaluated in 16 men with the primary obesity (body mass index - BMI > 43 kg/m2; waist-to-hip circumference ratio - WHR > 1.0) and in 17 healthy volunteers with normal body weight. The mean 24-h MEL level was significantly higher in obese patients than in healthy control individuals. Moreover, all obese men showed some abnormalities of MEL circadian pattern such as decreased ratio between day and night MEL levels, abnormal secretory peaks during the light hours and lower interindividual variability for timing amplitude. Abnormal circadian variations of MEL were associated with reduced 24-h mean values of LH, FSH, T and SHBG, whereas E2 levels were elevated. (author). 49 refs, 4 figs, 2 tabs

  12. Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

    DEFF Research Database (Denmark)

    Dam, Svend Secher; Thaysen-Andersen, Morten; Stenkjær, Eva; Lorentzen, Andrea; Roepstorff, Peter; Packer, Nicolle H; Stougaard, Jens

    2013-01-01

    Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial...... interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed...... using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (?20%), pauci-mannosidic (?40%), and complex forms (?40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants...

  13. Effect of triiodothyronine (L-T/sub 3/) and weight loss on sex-hormone binding globulin (SHBG) in hirsute obese women

    Energy Technology Data Exchange (ETDEWEB)

    Cavaliere, H.; Medeiros Neto, G.A. (Sao Paulo Univ. (Brazil). Faculdade de Medicina)

    1984-06-01

    The effect of a 60-day period of treatment with L-T/sub 3/ (200 ..mu..g/day) was evaluated in hirsute obese women and normal controls. The obese patients were also submitted to a low calorie high protein diet. Mean initial weight significantly declined with a significant lowering of the mean DHEA-S level. There were changes in serum T/sub 3/ which rose in both obese and normal patients. Serum sex-hormone binding globulin levels at baseline studies were significantly higher in normal controls than in obese patients. At the end of the 60-day period of L-T/sub 3/ administration it was observed in both groups a 2-4 fold increase in serum SHBG levels but normal controls had significantly higher mean values than obese individuals.

  14. The effect of triiodothyronine (L-T3) and weight loss on sex-hormone binding globulin (SHBG) in hirsute obese women

    International Nuclear Information System (INIS)

    The effect of a 60-day period of treatment with L-T3 (200 ?g/day) was evaluated in hirsute obese women and normal controls. The obese patients were also submitted to a low calorie high protein. Mean initial weight significantly declined with a significant lowering of the mean DHEA-S leves. There was changes in serum T3 rose in both obese and normal patients. Serum sex-hormone binding globulin levels at baseline studies were significantly higher in normal controls than in obese patients. At the end of de 60-day period of L-T3 administration it was observed in both groups a 2-4 fold increase in serum SHBG levels but normal controls had significantly higher mean values than obese individuals. (M.A.C.)

  15. Lipid Storage Diseases

    Science.gov (United States)

    ... Career Awards Enhancing Diversity Find People About NINDS Lipid Storage Diseases Fact Sheet See a list of ... Where can I get more information? What are lipid storage diseases? Lipid storage diseases, or the lipidoses , ...

  16. Globulina beta do líquido cefalorraqueano no prognóstico de processos inflamatórios do sistema nervoso central / The beta-globulin content of the cerebrospinal fluid and the evaluation of the prognosis in inflammatory diseases of the central nervous system

    Scientific Electronic Library Online (English)

    A., Spina-França.

    1964-03-01

    Full Text Available Foi feita avaliação do comportamento da globulina beta do LCR em processos inflamatórios do SNC e/ou de seus envoltórios no sentido de verificar até que ponto podem ser úteis para o prognóstico as informações obtidas. Essa avaliação foi baseada no fato de a concentração dessa globulina no LCR estar [...] relacionada ao metabolismo do parênquima nervoso, aumentando em condições que acarretem seu sofrimento. Os proteinogramas do LCR de 45 pacientes com processos inflamatórios do SNC e/ou de seus envoltórios, distribuídos em 6 grupos de casos, foram analisados segundo a possibilidade de o processo inflamatório estar acarretando, ou não, sofrimento do parênquima encefálico. A análise dos resultados mostrou que o estudo da concentração da globulina beta no LCR fornece elementos que permitem avaliar o comprometimento do parênquima encefálico nessas condições, aduzindo dados úteis para a avaliação prognóstica. A intensidade do aumento da concentração dessa globulina no LCR era maior do que a do aumento da concentração de albumina nos casos em que o processo inflamatório determinava comprometimento do parênquima encefálico. Abstract in english The CSF proteins of 45 patients with inflammatory diseases of the CNS were studied in order to evaluate the information that the beta-globulin content may bring about the occurrence of involvement of brain tissue. The material was distributed in 6 groups according to the diagnosis of the cases: 10 p [...] atients had spinal cord and/or radicular inflammatory disease, associated or not with leptomeningeal involvement (group 1); 5 had acute leptomeningitis (group 2); in 5 the diagnosis of tuberculous meningo-encephalitis was made (group 3); in 10, of cysticercosis of the CNS (group 4); in 5, of neurosyphilis (group 5); in 10, of encephalitides (group 6). Total protein content was determined by the turbidimetric method of the trichloroacetic acid. The protein fractions were analyzed through paper strip electrophoresis. The results were evaluated in respect to normal values previously reported. The high gamma-globulin content of the CSF in inflammatory processes of the CNS hinders the evaluation of the changes occurring in the content of the other globulins; the interference of such factor is more marked when the inflammatory process is chronic. In such conditions the evaluation of the content of the other globulin fractions is better achieved by comparison with the albumin content, the values reported for each globulin being statistically compared to those obtained for the albumin fraction. By this procedure it was shown that in all 45 cases changes in the alpha-globulin content were not different from that found for the albumin fraction and it was concluded that these data bring no evidences indicating interference of other factors than those related to the blood-CSF barrier for the explanation of the changes in the alpha-globulins content of the samples studied. Significant differences were found in respect to beta and gamma globulins. The changes found in the gamma-globulin support the possibility that the increase of this globulin is conditioned by the local production. Data concerning to beta-globulin in the cases of groups 5 and 6 showed that the increases in the amount of this globulin were more marked than those observed for the albumin. This difference was statistically significant. It brings evidence of participation of a different factor in the explanation of the finding other than those accepted for the albumin fraction. The discussion on the nature of this factor supports the possibility of its relation to the changes in the protein metabolism of the brain, since the damage of the latter was present in the cases of these groups of patients. These data are in agreement to the findings on the changes registered in the content of this globulin in the CSF in degenerative diseases as it is reported in the literature. The changes in the beta-globulin content of the CSF which are more marked than those found in the albumin con

  17. Effect of chickpea (Cicer arietinum L. germination on the major globulin content and in vitro digestibility Efeito da germinação de grão-de-bico (Cicer arietinum L. na globulina majoritária e digestibilidade in vitro

    Directory of Open Access Journals (Sweden)

    Guilherme Vanucchi Portari

    2005-12-01

    Full Text Available Chickpea seed germination was carried out over a period of 6 days. Little variation in the nitrogen and total globulin content was observed. The major globulin (11 S type showed higher variation after the 4th day of germination. The elution behaviour and distribution of the isolated major globulin fraction on Sepharose CL-6B chromatography showed little modification at the end of germination. On SDS-PAGE the peak eluted from Sepharose CL-6B showed changes in protein bands between 20 and 30 kDa and above 60 kDa, indicating protein degradation during the period. Proteolytic activity was detected in the albumin fraction of the seeds, which increased up to the fourth and then decreased up to the sixth day, when isolated chickpea total globulin and casein were used as substrates. Chickpea flour, isolated albumin and total globulin fractions did not show an increase for in vitro digestibility; however, the isolated major globulin was more susceptible to hydrolysis after germination.A germinação das sementes de grão-de-bico foi acompanhada por um período de 6 dias, no qual pequenas variações nos teores de nitrogênio e globulina total foram registradas. A globulina majoritária (tipo 11 S apresentou maiores variações após o quarto dia de germinação. A natureza e distribuição da fração globulina majoritária isolada na cromatografia em Sepharose CL-6B mostrou pequenas modificações ao final do período de germinação. A eletroforese em gel de poliacrilamida com dodecilssulfato de sódio do pico eluído na cromatografia em Sepharose CL-6B demonstra modificações nas bandas de proteínas entre os pesos moleculares de 20 e 30 kDa e acima de 60 kDa, indicando degradação protéica durante o período. Atividade proteolítica foi detectada na fração albumina da semente que aumentou até o quarto dia, seguido de queda até o sexto dia de germinação, quando da utilização de globulina total isolada da semente e caseína como substratos. Farinha de grão-de-bico, frações albumina e globulina total isoladas não apresentaram aumento na digestibilidade in vitro; entretanto, a fração globulina majoritária isolada foi mais suscetível à hidrólise após germinação.

  18. A simple and rapid Hepatitis A Virus (HAV titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations

    Directory of Open Access Journals (Sweden)

    Kaplan Gerardo G

    2008-12-01

    Full Text Available Abstract Background Hepatitis A virus (HAV, the causative agent of acute hepatitis in humans, is an atypical Picornaviridae that grows poorly in cell culture. HAV titrations are laborious and time-consuming because the virus in general does not cause cytopathic effect and is detected by immunochemical or molecular probes. Simple HAV titration assays could be developed using currently available viral construct containing selectable markers. Results We developed an antibiotic resistance titration assay (ARTA based on the infection of human hepatoma cells with a wild type HAV construct containing a blasticidin (Bsd resistance gene. Human hepatoma cells infected with the HAV-Bsd construct survived selection with 2 ?g/ml of blasticidin whereas uninfected cells died within a few days. At 8 days postinfection, the color of the pH indicator phenol red in cell culture media correlated with the presence of HAV-Bsd-infected blasticidin-resistant cells: an orange-to-yellow color indicated the presence of growing cells whereas a pink-to-purple color indicated that the cells were dead. HAV-Bsd titers were determined by an endpoint dilution assay based on the color of the cell culture medium scoring orange-to-yellow wells as positive and pink-to-purple wells as negative for HAV. As a proof-of-concept, we used the ARTA to evaluate the HAV neutralization potency of two commercially available human immune globulin (IG preparations and a WHO International Standard for anti-HAV. The three IG preparations contained comparable levels of anti-HAV antibodies that neutralized approximately 1.5 log of HAV-Bsd. Similar neutralization results were obtained in the absence of blasticidin by an endpoint dilution ELISA at 2 weeks postinfection. Conclusion The ARTA is a simple and rapid method to determine HAV titers without using HAV-specific probes. We determined the HAV neutralization potency of human IG preparations in 8 days by ARTA compared to the 14 days required by the endpoint dilution ELISA. The ARTA reduced the labour, time, and cost of HAV titrations making it suitable for high throughput screening of sera and antivirals, determination of anti-HAV antibodies in human immune globulin preparations, and research applications that involve the routine evaluation of HAV titers.

  19. Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK kernel globulin Caracterizaçao funcional das globulinas de amêndoa de castanha-do-Pará após a acetilação

    Directory of Open Access Journals (Sweden)

    Cíntia Maria Pinto Ramos

    2004-03-01

    Full Text Available Defatted Brazil nut kernel flour, a rich source of high quality proteins, is presently being utilized in the formulation of animal feeds. One of the possible ways to improve its utilization for human consumption is through improvement in its functional properties. In the present study, changes in some of the functional properties of Brazil nut kernel globulin were evaluated after acetylation at 58.6, 66.2 and 75.3% levels. The solubility of acetylated globulin was improved above pH 6.0 but was reduced in the pH range of 3.0-4.0. Water and oil absorption capacity, as well as the viscosity increased with increase in the level of acetylation. Level of modification also influenced the emulsifying capacity: decreased at pH 3.0, but increased at pH 7.0 and 9.0. Highest emulsion activity (approximately 62.2% was observed at pH 3.0 followed by pH 9.0 and pH 7.0 and least (about 11.8% at pH 5.0. Emulsion stability also followed similar behavior as that of emulsion activity.Farinha desengordurada de amêndoa de castanha-do-Pará, fonte rica de proteína de alta qualidade, vem sendo, atualmente, aproveitada apenas na formulação de ração animal. Uma das possíveis maneiras de melhorar seu aproveitamento para o consumo humano é através do melhoramento de suas propriedades funcionais. No presente trabalho, mudanças em algumas propriedades funcionais da globulina de castanha-do-Pará, após acetilação, aos níveis de 58,6, 66,2 e 75,3% foram estudadas. A solubilidade da globulina acetilada aumentou acima de pH 6,0, porém diminuiu na faixa de pH 3,0 a 4,0. As capacidades de absorção de água e de óleo como também a viscosidade, melhoraram com o aumento de grau de acetilação. O grau de modificação também influenciou a capacidade de emulsificação: reduziu em pH 3,0, e aumentou nos pHs 7,0 e 9,0. A máxima atividade de emulsão (aproximadamente 62,2% foi observada em pH 3,0 seguida de pH 9,0 e a mínima foi observada (11,8% em pH 5,0. A estabilidade de emulsão também apresentou o comportamento similar à atividade de emulsão.

  20. Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK) kernel globulin / Caracterizaçao funcional das globulinas de amêndoa de castanha-do-Pará após a acetilação

    Scientific Electronic Library Online (English)

    Cíntia Maria Pinto, Ramos; Pushkar Singh, Bora.

    2004-03-01

    Full Text Available Farinha desengordurada de amêndoa de castanha-do-Pará, fonte rica de proteína de alta qualidade, vem sendo, atualmente, aproveitada apenas na formulação de ração animal. Uma das possíveis maneiras de melhorar seu aproveitamento para o consumo humano é através do melhoramento de suas propriedades fun [...] cionais. No presente trabalho, mudanças em algumas propriedades funcionais da globulina de castanha-do-Pará, após acetilação, aos níveis de 58,6, 66,2 e 75,3% foram estudadas. A solubilidade da globulina acetilada aumentou acima de pH 6,0, porém diminuiu na faixa de pH 3,0 a 4,0. As capacidades de absorção de água e de óleo como também a viscosidade, melhoraram com o aumento de grau de acetilação. O grau de modificação também influenciou a capacidade de emulsificação: reduziu em pH 3,0, e aumentou nos pHs 7,0 e 9,0. A máxima atividade de emulsão (aproximadamente 62,2%) foi observada em pH 3,0 seguida de pH 9,0 e a mínima foi observada (11,8%) em pH 5,0. A estabilidade de emulsão também apresentou o comportamento similar à atividade de emulsão. Abstract in english Defatted Brazil nut kernel flour, a rich source of high quality proteins, is presently being utilized in the formulation of animal feeds. One of the possible ways to improve its utilization for human consumption is through improvement in its functional properties. In the present study, changes in so [...] me of the functional properties of Brazil nut kernel globulin were evaluated after acetylation at 58.6, 66.2 and 75.3% levels. The solubility of acetylated globulin was improved above pH 6.0 but was reduced in the pH range of 3.0-4.0. Water and oil absorption capacity, as well as the viscosity increased with increase in the level of acetylation. Level of modification also influenced the emulsifying capacity: decreased at pH 3.0, but increased at pH 7.0 and 9.0. Highest emulsion activity (approximately 62.2%) was observed at pH 3.0 followed by pH 9.0 and pH 7.0 and least (about 11.8%) at pH 5.0. Emulsion stability also followed similar behavior as that of emulsion activity.

  1. Sex hormone binding globulin decrease as potential pathogenetic factor for hirsutism in adolescent girls / Disminución de la globulina transportadora de hormonas sexuales como factor patogénico de hirsutismo en la adolescencia

    Scientific Electronic Library Online (English)

    Graciela, Cross; Karina, Danilowics; Martha, Kral; Anne, Caufriez; Georges, Copinschi; Oscar D., Bruno.

    2008-04-01

    Full Text Available Se investigaron 252 mujeres con peso normal, de 13 a 39 años de edad, para evaluar si un descenso exagerado en los niveles de la globulina transportadora de hormonas sexuales ("sex hormone binding globulin"; SHBG) puede tener un rol en el desarrollo de hirsutismo. Este signo fue evaluado con la esca [...] la de Ferriman y Gallwey (FG), empleando un criterio riguroso de normalidad 4) y controles (FG Abstract in english We investigated 252 non-obese female subjects aged 13-39 years to evaluate if an exaggerated descent of sex hormone binding globulin (SHBG) levels during adolescence can play a role in the development of hirsutism. Body hair was assessed according to Ferriman and Gallwey (FG), with a stringent crite [...] rion of normality of 4) and controls (FG

  2. Functional Implication of an Arg307Gly Substitution in Corticosteroid-Binding Globulin, a Candidate Gene for a Quantitative Trait Locus Associated With Cortisol Variability and Obesity in Pig

    OpenAIRE

    Guyonnet-Dupérat, Véronique; Geverink, Nicoline; Plastow, Graham S.; Evans, Gary; Ousova, Olga; Croisetière, Christian; Foury, Aline; Richard, Elodie; Mormède, Pierre; Moisan, Marie-Pierre

    2006-01-01

    We previously reported that corticosteroid-binding globulin gene (Cbg) may be the causal gene of a quantitative trait locus associated with cortisol levels, fat deposition, and muscle content in a pig intercross. Sequence analysis of parental animals allowed us to identify four amino-acid substitutions. Here we have examined if any of these single amino acid substitutions could be responsible for the difference in CBG binding and affinity for cortisol between the parental breeds, using in vit...

  3. The role of sex hormone binding globulin (SHBG) in conversion of testosterone to 5 ?-dihydrotestosterone and androstenedione in skin from suprapubic area of women. Part 2. In vitro study with skin homogenates

    International Nuclear Information System (INIS)

    The influence of the sex hormone binding globulin (SHBG) and of microsomal supernatant (cytosol) on 3H-testosterone transformation into 5 alpha-dihydrotestosterone by skin microsomes of the suprapubic area of women in reproductive age was studied. Microsomal fractions were incubated with different forms of cytosol and with active and inactive globulin. Steroids, following isolation from the incubation material, were purified with the use of column and thin layer chromotographies. Individual recovery of identified androstenedione was calculated using radioisotopes, and the recovery of 5 alpha-dihydrotestosterone was determined with gas chromatography. The transformation of 3H-testosterone into 5 alpha-dihydrotestosterone and androstenedione by skin microsomes in the presence of active globulin has become significantly reduced. Microsomal supernatant (cytosol) has not changed the quantity of identified 5 alpha-dihydrotestosterone, whereas the quantity of formed androstenedione has slightly decreased. The lack of cyprosterone influence on 5 alpha reductase activity and a slight decrease in 17-dehydrogenase activity in the skin microsomal fraction were found. (author)

  4. Corticosteroid-binding globulin (CBG) reactive centre loop antibodies and surface plasmon resonance interrogate the proposed heat dependent "flip-flop" mechanism of human CBG.

    Science.gov (United States)

    Lewis, John G; Fredericks, Rayleen; Fee, Conan J; Elder, Peter A

    2016-04-01

    Corticosteroid-binding globulin (CBG) is the predominant carrier of cortisol in circulation and is a non-inhibitory member of the serpin superfamily of serine protease inhibitors. In the stressed or "S" conformation, CBG possesses an intact exposed reactive centre loop (RCL) that can be irreversibly cleaved by elastase released from activated human neutrophils whereupon it adopts a relaxed or "R" conformation. The latter conformation has decreased affinity for cortisol, allowing the release of the majority of cortisol at sites of inflammation. Recently there has been speculation that mild increments in heat such as found in pyrexia (39-40°C) may also induce a reversible "flip-flop" of the RCL into the body of the protein structure, without cleavage, facilitating a reversible temperature-dependent release of cortisol. Here we raised a new monoclonal antibody to the RCL of human CBG and used this in concert with an existing RCL antibody and show by surface plasma resonance that, at temperatures up to 40°C, the RCL of purified CBG and the RCL of CBG in intact plasma is accessible to these two antibodies. Together, the epitopes of these antibodies span 11 consecutive amino acids (STGVTLNLTSK) of the 18 residues of the RCL. This adequate antibody cover of the RCL sequence leads to the conclusion that the proposed temperature-dependent "flip-flop" of the RCL of CBG is doubtful. PMID:26829559

  5. Influx of testosterone-binding globulin (TeBG) and TeBG-bound sex steroid hormones into rat testis and prostate

    International Nuclear Information System (INIS)

    The availability of testosterone and estradiol to Sertoli and prostate cells is dependent upon 1) the permeability properties of the blood-tubular barrier (BTB) of the testis or prostate cell membrane, and 2) sex steroid binding to plasma proteins, such as albumin or testosterone-binding globulin (TeBG). Sex steroid influx into these tissues was studied after in vivo arterial bolus injections of [3H]testosterone or [3H]estradiol in anesthetized rats. Both testosterone and estradiol were readily cleared across the BTB or prostate cell membrane in the absence of plasma proteins and in the presence of human pregnancy serum, in which testosterone or estradiol are 80-95% distributed to TeBG. The extravascular extraction of [3H]TeBG across the BTB or prostate plasma membrane [73 +/- 2% (+/- SE) and 92 +/- 9%, respectively] was significantly greater than extraction of [3H]albumin or other plasma space markers and indicative of a rapid first pass clearance of TeBG by Sertoli or prostate cells. In summary, these studies indicate that 1) testosterone and estradiol are readily cleared by Sertoli and prostate cells; 2) albumin- and TeBG-bound sex steroids represent the major circulating pool of bioavailable hormone for testis or prostate; and 3) the TeBG-sex steroid complex may be nearly completely available for influx through the BTB or prostate plasma membrane

  6. Ovarian cancer risk and common variation in the sex hormone-binding globulin gene: a population-based case-control study

    Directory of Open Access Journals (Sweden)

    Yeager Meredith

    2007-04-01

    Full Text Available Abstract Background The sex hormone-binding globulin (SHBG is a carrier protein that modulates the bio-availability of serum sex steroid hormones, which may be involved in ovarian cancer. We evaluated whether common genetic variation in SHBG and its 3' neighbor ATP1B2, in linkage disequilibrium, is associated with the risk of epithelial ovarian cancer. Methods The study population included 264 women with ovarian carcinoma and 625 controls participating in a population-based case-control study in Poland. Five common single nucleotide polymorphisms (SNPs in SHGB and five in ATP1B2 were selected to capture most common variation in this region. Results None of the SNPs evaluated was significantly associated with ovarian cancer risk, including the putative functional SNPs SHBG D356N (rs6259 and -67G>A 5'UTR (rs1799941. However, our data were consistent with a decreased ovarian cancer risk associated with the variant alleles for these two SNPs, which have been previously associated with increased circulating levels of SHBG. Conclusion These data do not support a substantial association between common genetic variation in SHBG and ovarian cancer risk.

  7. Elevation of corticosteroid-binding globulin in Obese strain (OS) chickens: possible implications for the disturbed immunoregulation and the development of spontaneous autoimmune thyroiditis

    International Nuclear Information System (INIS)

    Basal plasma levels of corticosterone and corticosteroid-binding globulin (CBG) have been investigated in Obese strain (OS) chickens afflicted with spontaneous autoimmune thyroiditis (SAT). Corticosterone was determined radioimmunologically, and CBG by using a highly sensitive radioligand saturation assay. OS chickens displayed total corticosterone levels not different from healthy normal White Leghorn (NWL) chickens. CBG, however, was found to be twice as high in OS chickens as compared with their healthy counterparts, irrespective of sex or age. This quantitative difference in the CBG level is not compensated for by either altered affinity or specificity of the molecule. Furthermore, no differences were found in the response of OS and NWL lymphocytes to the suppressive effect of glucocorticoids in vitro. It was therefore assumed that OS animals are deficient in free, hormonally active corticosterone. An additional indication for such a diminished glucocorticoid tonus was that in vivo treatment of OS chickens with glucocorticoid hormones, thus increasing the free and active hormone fraction, normalizes the T cell hyperreactivity and significantly reduces thyroid infiltration. Possible pathophysiological implications of a diminished glucocorticoid tonus for spontaneous autoimmunity, as well as possible explanations for the beneficial effects of glucocorticoid treatment on the development of SAT, are discussed

  8. Elevation of corticosteroid-binding globulin in Obese strain (OS) chickens: possible implications for the disturbed immunoregulation and the development of spontaneous autoimmune thyroiditis

    Energy Technology Data Exchange (ETDEWEB)

    Faessler, R.; Schauenstein, K.; Kroemer, G.; Schwarz, S.; Wick, G.

    1986-05-15

    Basal plasma levels of corticosterone and corticosteroid-binding globulin (CBG) have been investigated in Obese strain (OS) chickens afflicted with spontaneous autoimmune thyroiditis (SAT). Corticosterone was determined radioimmunologically, and CBG by using a highly sensitive radioligand saturation assay. OS chickens displayed total corticosterone levels not different from healthy normal White Leghorn (NWL) chickens. CBG, however, was found to be twice as high in OS chickens as compared with their healthy counterparts, irrespective of sex or age. This quantitative difference in the CBG level is not compensated for by either altered affinity or specificity of the molecule. Furthermore, no differences were found in the response of OS and NWL lymphocytes to the suppressive effect of glucocorticoids in vitro. It was therefore assumed that OS animals are deficient in free, hormonally active corticosterone. An additional indication for such a diminished glucocorticoid tonus was that in vivo treatment of OS chickens with glucocorticoid hormones, thus increasing the free and active hormone fraction, normalizes the T cell hyperreactivity and significantly reduces thyroid infiltration. Possible pathophysiological implications of a diminished glucocorticoid tonus for spontaneous autoimmunity, as well as possible explanations for the beneficial effects of glucocorticoid treatment on the development of SAT, are discussed.

  9. Analysis of Hormone-Protein Binding in Solution by Ultrafast Affinity Extraction: Interactions of Testosterone with Human Serum Albumin and Sex Hormone Binding Globulin.

    Science.gov (United States)

    Zheng, Xiwei; Bi, Cong; Brooks, Marissa; Hage, David S

    2015-11-17

    Ultrafast affinity extraction was used to study hormone-protein interactions in solution, using testosterone and its transport proteins human serum albumin (HSA) and sex hormone binding globulin (SHBG) as models. Both single column and two-dimensional systems based on HSA microcolumns were utilized to measure the free fraction of testosterone in hormone/protein mixtures at equilibrium or that were allowed to dissociate for various lengths of time. These data were used to determine the association equilibrium constants (Ka) or global affinities (nKa') and dissociation rate constants (kd) for testosterone with soluble HSA and SHBG. This method was also used to measure simultaneously the free fraction of testosterone and its equilibrium constants with both these proteins in physiological mixtures of these agents. The kd and Ka values obtained for HSA were 2.1-2.2 s(-1) and 3.2-3.5 × 10(4) M(-1) at pH 7.4 and 37 °C. The corresponding constants for SHBG were 0.053-0.058 s(-1) and 0.7-1.2 × 10(9) M(-1). All of these results gave good agreement with literature values, indicating that this approach could provide information on a wide range of rate constants and binding strengths for hormone-protein interactions in solution and at clinically relevant concentrations. The same method could be extended to alternative hormone-protein systems or other solutes and binding agents. PMID:26484387

  10. Plasma "cold-insoluble globulin" protects cytotoxic lymphocytes from ATP inhibition: 2. Immunization against viral cell surface antigen stimulates cytotoxic cells to lyse tumor cells.

    Science.gov (United States)

    Hakim, A A; Siraki, C M

    1986-01-01

    The present studies examined the immunoregulatory factors that modulate T-lymphocyte cytotoxic activity against tumor cells. At 30 days postvaccination with hepatitis B virus surface antigen(s) (HBsAg), peripheral blood lymphocytes (PBL) from HBsAb-seropositive healthy donors showed 8-10-fold increase in their cytotoxic activity against human hepatocellular carcinoma PLC/PRF/5 cells. However, there was no effect against human embryonic liver or lung fibroblasts (WI-35) cells. After cloning, these cytotoxic lymphocytes responded to HBsAg, phytohemagglutinin (PHA), and neuraminidase (VCN) with significant increases in cytotoxicity. These cloned, activated PBL also had no effect on human embryonic liver or lung fibroblasts. Exogenous 5'-triphosphate (ATP) inhibited the lymphocyte cytotoxic activities. The inhibition was ATP concentration-dependent. Also, colchicine, a tubuline depolymerizing agent, inhibited effector cell cytotoxic lymphocyte activity. Colchicine binds to the target cells without causing cell lysis. Preincubation of the cytotoxic lymphocytes with "cold-insoluble globulin" (CIg) protected the cells from ATP and colchicine inhibition. PMID:3017558

  11. Anti-thymocyte globulin could improve the outcome of allogeneic hematopoietic stem cell transplantation in patients with highly aggressive T-cell tumors

    International Nuclear Information System (INIS)

    The early experiment result in our hospital showed that anti-thymocyte globulin (ATG) inhibited the proliferation of lymphoid tumor cells in the T-cell tumors. We used the ATG as the part of the conditioning regimen and to evaluate the long-term anti-leukemia effect, the safety and complication in the patients with highly aggressive T-cell lymphomas. Twenty-three patients were enrolled into this study. At the time of transplant, six patients reached first or subsequent complete response, three patients had a partial remission and 14 patients had relapsed or primary refractory disease. The conditioning regimen consisted of ATG, total body irradiation, toposide and cyclophosphamide. The complete remission rate after transplant was 95.7%. At a median follow-up time of 25 months, 16 (69.6%) patients are alive and free from diseases, including nine patients in refractory and progressive disease. Seven patients died after transplant, five from relapse and two from treatment-related complications. The incidence of grades II–IV acute graft-vs-host disease (GvHD) was 39.1%. The maximum cumulative incidence of chronic GvHD was 30%. The most frequent and severe conditioning-related toxicities observed in 8 out of 23 patients were grades III/IV infections during cytopenia. Thus, ATG-based conditioning is a feasible and effective alternative for patients with highly aggressive T-cell tumors

  12. Ovarian cancer risk and common variation in the sex hormone-binding globulin gene: a population-based case-control study

    International Nuclear Information System (INIS)

    The sex hormone-binding globulin (SHBG) is a carrier protein that modulates the bio-availability of serum sex steroid hormones, which may be involved in ovarian cancer. We evaluated whether common genetic variation in SHBG and its 3' neighbor ATP1B2, in linkage disequilibrium, is associated with the risk of epithelial ovarian cancer. The study population included 264 women with ovarian carcinoma and 625 controls participating in a population-based case-control study in Poland. Five common single nucleotide polymorphisms (SNPs) in SHGB and five in ATP1B2 were selected to capture most common variation in this region. None of the SNPs evaluated was significantly associated with ovarian cancer risk, including the putative functional SNPs SHBG D356N (rs6259) and -67G>A 5'UTR (rs1799941). However, our data were consistent with a decreased ovarian cancer risk associated with the variant alleles for these two SNPs, which have been previously associated with increased circulating levels of SHBG. These data do not support a substantial association between common genetic variation in SHBG and ovarian cancer risk

  13. Complete thyroxine-binding globulin (TBG) deficiency produced by a mutation in acceptor splice site causing frameshift and early termination of translation (TBG-Kankakee).

    Science.gov (United States)

    Carvalho, G A; Weiss, R E; Refetoff, S

    1998-10-01

    Fourteen T4-binding globulin (TBG) variants have been identified at the gene level. They are all located in the coding region of the gene and 6 produce complete deficiency of TBG (TBG-CD). We now describe the first mutation in a noncoding region producing TBG-CD. The proband was treated for over 20 yr with L-T4 because of fatigue associated with a low concentration of serum total T4. Fifteen family members were studied showing low total T4 inherited as an X chromosome-linked trait, and affected males had undetectable TBG in serum. Sequencing of the entire coding region and promoter of the TBG gene revealed no abnormality. However, an A to G transition was found in the acceptor splice junction of intron II that produced a new HaeIII restriction site cosegregating with the TBG-CD phenotype. Sequencing exon 1 to exon 3 of TBG complementary DNA reverse transcribed from messenger RNA of skin fibroblasts from an affected male, confirmed a shift in the ag acceptor splice site. This results in the insertion of a G in exon 2 and causes a frameshift and a premature stop at codon 195. This early termination of translation predicts a truncated TBG lacking 201 amino acids. PMID:9768672

  14. Abstract Storage Devices

    OpenAIRE

    Koenig, Robert; Maurer, Ueli; Tessaro, Stefano

    2007-01-01

    A quantum storage device differs radically from a conventional physical storage device. Its state can be set to any value in a certain (infinite) state space, but in general every possible read operation yields only partial information about the stored state. The purpose of this paper is to initiate the study of a combinatorial abstraction, called abstract storage device (ASD), which models deterministic storage devices with the property that only partial information about the state can be re...

  15. Fuel storage systems

    Energy Technology Data Exchange (ETDEWEB)

    Donakowski, T.D.; Tison, R.R.

    1979-08-01

    Storage technologies are characterized for solid, liquid, and gaseous fuels. Emphasis is placed on storage methods applicable to Integrated Community Energy Systems based on coal. Items discussed here include standard practice, materials and energy losses, environmental effects, operating requirements, maintenance and reliability, and cost considerations. All storage systems were found to be well-developed and to represent mature technologies; an exception may exist for low-Btu gas storage, which could have materials incompatability.

  16. A generic storage API

    OpenAIRE

    Kirby, Graham; Zirintsis, Evangelos; Dearle, Alan; Morrison, Ron

    2003-01-01

    We present a generic API suitable for provision of highly generic storage facilities that can be tailored to produce various individually customised storage infrastructures. The paper identifies a candidate set of minimal storage system building blocks, which are sufficiently simple to avoid encapsulating policy where it cannot be customised by applications, and composable to build highly flexible storage architectures. Four main generic components are defined: the store, the namer, the caste...

  17. A Generic Storage API

    OpenAIRE

    2010-01-01

    We present a generic API suitable for provision of highly generic storage facilities that can be tailored to produce various individually customised storage infrastructures. The paper identifies a candidate set of minimal storage system building blocks, which are sufficiently simple to avoid encapsulating policy where it cannot be customised by applications, and composable to build highly flexible storage architectures. Four main generic components are defined: the store, the namer, the caste...

  18. Pit Water Storage Ottrupgaard

    DEFF Research Database (Denmark)

    Heller, Alfred

    The pit water storage, a seasonal thermal storage, was built in 1993 with floating lid and hybrid clay-polymer for pit lining. The storage was leaking severe and solutions were to be found. In the paper solutions for pit lining and floating lids are discussed, cost estimations given and coming...

  19. Fuel storage tank

    International Nuclear Information System (INIS)

    The stationary fuel storage tank is immersed below the water level in the spent fuel storage pool. In it there is placed a fuel assembly within a cage. Moreover, the storage tank has got a water filling and a gas buffer. The water in the storage tank is connected with the pool water by means of a filter, a surge tank and a water purification facility, temperature and pressure monitoring being performed. In the buffer compartment there are arranged catalysts a glow plugs for recombination of radiolysis products into water. The supply of water into the storage tank is performed through the gas buffer compartment. (DG)

  20. Cloud storage for dummies

    CERN Document Server

    Xu, Linda; Loughlin, Tanya

    2010-01-01

    Understand cloud computing and save your organization time and money! Cloud computing is taking IT by storm, but what is it and what are the benefits to your organization? Hitachi Data Systems' Cloud Storage For Dummies provides all the answers, With this book, you discover a clear explanation of cloud storage, and tips for how to choose the right type of cloud storage for your organization's needs. You also find out how cloud storage can free up valuable IT resources, saving time and money. Cloud Storage For Dummies presents useful information on setting up a

  1. Assessing storage adequacy

    International Nuclear Information System (INIS)

    Government policy encourages the use of natural gas. It is expected that liquefied natural gas (LNG) and Arctic gas will make up 20 to 25 per cent of supply. This presentation provided an outlook of storage value based on a technical analysis by the National Petroleum Counsel (NPC) report. A moderately robust growth is expected in the residential and commercial load which may be partially offset by robust growth in electricity. The net result is an increase in storage requirements. It was concluded that there is a strong case for growth in storage demand but a lack of good sites for additional capacity. This will lead to higher storage values. The NPC sees the need for 1 Tcf more storage use by 2025, of which 700 Bcf will need to come from new storage. In particular, current storage levels may not be sufficient to meet a colder than normal winter, and deliverability is affected by field inventory. Most storage capacity was built before 1985, mostly by regulated entities. It is expected that only 250 to 400 Bcf will be added over the next 25 years in North America. If storage becomes scarce, prices will move to the marginal cost of new additions, and the upper limit on price will be determined by salt cavern storage. An increase of $1.00 in the price of leasing storage would add about $0.11 to the average price of consumed gas. tabs., figs

  2. Energy storage; Energiespeicher

    Energy Technology Data Exchange (ETDEWEB)

    Stenzel, Peter; Bongartz, Richard; Fleer, Johannes; Hennings, Wilfried; Linssen, Jochen [Forschungszentrum Juelich (Germany). Institut fuer Energie- und Klimaforschung - Systemforschung und Technologische Entwicklung (IEK-STE)

    2013-04-01

    Energy storage facilities are an important component in energy supply systems. Energy storage facilities are the state of the art in different fields of applications such as energy trading, emergency power supply or supply for individual suppliers. Energy storage facilities are supplied for decades. However, in conjunction with the energy policy turnaround, the structural transformation processes of the German energy system result in a changed role for energy storage facilities. This is particularly apparent on the one hand in an increasing demand for energy storage covering the total area of (ultra)short-time storages up to long-term storages. On the other hand, new fields of application appear. Modifications based on adapted regulatory framework conditions have to be considered for established fields of application. Stationary battery storages may play an important role in the future portfolio of storage facilities. In the last years, a wide dynamic of research activities is observed due to an increased number of pilot projects (such as battery storages for the allocation of service systems). Also a wide range of commercial products especially in the range of home energy storage in order to increase the own consumption of photovoltaic power is available on the market meanwhile.

  3. A Survey on Cloud Storage

    OpenAIRE

    Jiehui Ju; Jiyi Wu; Jianqing Fu; Zhijie Lin; Jianlin Zhang

    2011-01-01

    Cloud storage is a new concept come into being simultaneously with cloud computing, and can be divided into public cloud storage, private cloud storage and hybrid cloud storage. This article gives a quick introduction to cloud storage. It covers the key technologies in Cloud Computing and Cloud Storage. Google GFS massive data storage system and the popular open source Hadoop HDFS were detailed introduced to analyze the principle of Cloud Storage technology. As an important technology area an...

  4. Pump storage. Requirements and comparsion with other storage technologies

    Energy Technology Data Exchange (ETDEWEB)

    Ruprecht, Albert [Stuttgart Univ. (Germany). Inst. of Fluid Mechanics and Hydraulic Machinery

    2015-05-01

    The use of renewable energy sources has strongly increased in recent years. Because of the high volatility of these sources, an appropriate energy storage capacity as well as the provision of fast and flexible regulating power is necessary. Both can be supplied by pump storage power plants. The pump storage situation is Germany is discussed. The demand of energy storage is roughly estimated and the status quo of pump storage in Germany is described. Pump storage is compared to other storage technologies (compressed air storage, mobile batteries, hydrogen storage and power-to-gas storage) in terms of efficiency, response time and costs.

  5. Pump storage. Requirements and comparsion with other storage technologies

    International Nuclear Information System (INIS)

    The use of renewable energy sources has strongly increased in recent years. Because of the high volatility of these sources, an appropriate energy storage capacity as well as the provision of fast and flexible regulating power is necessary. Both can be supplied by pump storage power plants. The pump storage situation is Germany is discussed. The demand of energy storage is roughly estimated and the status quo of pump storage in Germany is described. Pump storage is compared to other storage technologies (compressed air storage, mobile batteries, hydrogen storage and power-to-gas storage) in terms of efficiency, response time and costs.

  6. Accumulation of plant small heat-stress proteins in storage organs.

    Science.gov (United States)

    Lubaretz, Olga; Zur Nieden, Uta

    2002-06-01

    Plant small heat-stress proteins (sHSPs) have been shown to be expressed not only after exposure to elevated temperatures, but also at particular developmental stages such as embryogenesis, microsporogenesis, and fruit maturation. This paper presents new data on the occurrence of sHSPs in vegetative tissues, their tissue-specific distribution, and cellular localization. We have found sHSPs in 1-year-old twigs of Acer platanoides L. and Sambucus nigra L. and in the liana Aristolochia macrophylla Lamk. exclusively in the winter months. In tendrils of Aristolochia, sHSPs were localized in vascular cambium cells. After budding, in spring, these proteins were no longer present. Furthermore, accumulation of sHSPs was demonstrated in tubers and bulbs of Allium cepa L., Amaryllis ( Hippeastrum hybridum hort.), Crocus albiflorus L., Hyacinthus orientalis L., Narcissus pseudonarcissus L., Tulipa gesneriana L., and Solanum tuberosum L. (potato). In potato tubers and bulb scales of Narcissus the stress proteins were localized in the central vacuoles of storage parenchyma cells. In order to obtain more information on a possible functional correlation between storage proteins and sHSPs, the accumulation of both types of protein in tobacco seeds during seed ripening and germination was monitored. The expression of sHSPs and globulins started simultaneously at about the 17th day after anthesis. During seed germination the sHSPs disappeared in parallel with the storage proteins. Furthermore, in embryos of transgenic tobacco plants, which do not contain any protein bodies or storage proteins, no sHSPs were found. Thus, the occurrence of sHSPs in perennial plant storage organs seems to be associated with the presence of storage proteins. PMID:12029471

  7. Associations of sex hormone-binding globulin and testosterone with diabetes among men and women (the Saku Diabetes study: a case control study

    Directory of Open Access Journals (Sweden)

    Goto Atsushi

    2012-10-01

    Full Text Available Abstract Background Sex hormone-binding globulin (SHBG levels and sex hormones have been implicated in the pathogenesis of type 2 diabetes and cardiovascular diseases. As fatty liver has been suggested to be a major determinant of SHBG levels, we examined whether the associations of SHBG and testosterone with diabetes were independent of fatty liver. Methods We conducted a case–control study that included 300 diabetes cases (215 men and 85 women and 300 matched controls from the Saku cohort study. Diabetes was defined by either fasting plasma glucose levels ?126 mg/dL, 2-h post-load glucose levels ?200 mg/dL after a 75 g oral glucose tolerance test, or diabetes diagnosed by physicians. We fitted conditional logistic regression models to examine the associations between SHBG and total testosterone levels with diabetes by sex. To evaluate the impact of fatty liver, we used the fatty liver index (FLI, a validated measure derived from serum triglyceride levels, body mass index (BMI, waist circumference, and ?-glutamyltransferase levels. Results After adjusting for age, family history of diabetes, smoking, physical activity, BMI, and FLI, SHBG levels were inversely associated with diabetes among women (odds ratio [OR] comparing the highest with the lowest quartiles, 0.13 [95% confidence interval {CI}, 0.02–0.96], but not among men. Similar patterns were observed in a subgroup analysis restricted to postmenopausal women"(OR, 0.12 [95% CI, 0.01–1.17]. In contrast, testosterone levels were inversely associated with diabetes among men (OR, 0.45 [95% CI, 0.23–0.89], but not among women. Conclusions Our findings suggest that SHBG in women and testosterone in men may be inversely associated with diabetes.

  8. Low levels of sex hormone-binding globulin and hyperproinsulinemia as markers of increased pancreatic ß-cell demand in men

    Directory of Open Access Journals (Sweden)

    A.F. Reis

    1998-12-01

    Full Text Available Low levels of sex hormone-binding globulin (SHBG are considered to be an indirect index of hyperinsulinemia, predicting the later onset of diabetes mellitus type 2. In the insulin resistance state and in the presence of an increased pancreatic ß-cell demand (e.g. obesity both absolute and relative increases in proinsulin secretion occur. In the present study we investigated the correlation between SHBG and pancreatic ß-cell secretion in men with different body compositions. Eighteen young men (30.0 ± 2.4 years with normal glucose tolerance and body mass indexes (BMI ranging from 22.6 to 43.2 kg/m2 were submitted to an oral glucose tolerance test (75 g and baseline and 120-min blood samples were used to determine insulin, proinsulin and C-peptide by specific immunoassays. Baseline SHBG values were significantly correlated with baseline insulin (r = -0.58, P28 kg/m2, N = 8 and nonobese (BMI £25 kg/m2, N = 10 groups, significantly lower levels of SHBG were found in the obese subjects. The obese group had significantly higher baseline proinsulin, C-peptide and 120-min proinsulin and insulin levels. For the first time using a specific assay for insulin determination, a strong inverse correlation between insulinemia and SHBG levels was confirmed. The finding of a strong negative correlation between SHBG levels and pancreatic ß-cell secretion, mainly for the 120-min post-glucose load proinsulin levels, reinforces the concept that low SHBG levels are a suitable marker of increased pancreatic ß-cell demand.

  9. The effects of caffeinated and decaffeinated coffee on sex hormone-binding globulin and endogenous sex hormone levels: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Wedick Nicole M

    2012-10-01

    Full Text Available Abstract Background Findings from observational studies suggest that sex hormone-binding globulin (SHBG and endogenous sex hormones may be mediators of the putative relation between coffee consumption and lower risk of type 2 diabetes. The objective of this study was to evaluate the effects of caffeinated and decaffeinated coffee on SHBG and sex hormone levels. Findings After a two-week run-in phase with caffeine abstention, we conducted an 8-week parallel-arm randomized controlled trial. Healthy adults (n?=?42 were recruited from the Boston community who were regular coffee consumers, nonsmokers, and overweight. Participants were randomized to five 6-ounce cups of caffeinated or decaffeinated instant coffee or water (control group per day consumed with each meal, mid-morning, and mid-afternoon. The main outcome measures were SHBG and sex hormones [i.e., testosterone, estradiol, dehydroepiandrosterone sulfate]. No significant differences were found between treatment groups for any of the studied outcomes at week 8. At 4 weeks, decaffeinated coffee was associated with a borderline significant increase in SHBG in women, but not in men. At week 4, we also observed several differences in hormone concentrations between the treatment groups. Among men, consumption of caffeinated coffee increased total testosterone and decreased total and free estradiol. Among women, decaffeinated coffee decreased total and free testosterone and caffeinated coffee decreased total testosterone. Conclusions Our data do not indicate a consistent effect of caffeinated coffee consumption on SHBG in men or women, however results should be interpreted with caution given the small sample size. This is the first randomized trial investigating the effects of caffeinated and decaffeinated coffee on SHBG and sex hormones and our findings necessitate further examination in a larger intervention trial.

  10. Hazard evaluation of chemicals that cause accumulation of alpha 2u-globulin, hyaline droplet nephropathy, and tubule neoplasia in the kidneys of male rats.

    Science.gov (United States)

    Hard, G C; Rodgers, I S; Baetcke, K P; Richards, W L; McGaughy, R E; Valcovic, L R

    1993-03-01

    This review paper examines the relationship between chemicals inducing excessive accumulation of alpha 2u-globulin (alpha 2u-g) (CIGA) in hyaline droplets in male rat kidneys and the subsequent development of nephrotoxicity and renal tubule neoplasia in the male rat. This dose-responsive hyaline droplet accumulation distinguishes CIGA carcinogens from classical renal carcinogens. CIGA carcinogens also do not appear to react with DNA and are generally negative in short-term tests for genotoxicity, CIGA or their metabolites bind specifically, but reversibly, to male rat alpha 2u-g. The resulting complex appears to be more resistant to hydrolytic degradation in the proximal tubule than native, unbound alpha 2u-g. Single cell necrosis of the tubule epithelium, with associated granular cast formation and papillary mineralization, is followed by sustained regenerative tubule cell proliferation, foci of tubule hyperplasia in the convoluted proximal tubules, and renal tubule tumors. Although structurally similar proteins have been detected in other species, including humans, renal lesions characteristic of alpha 2u-g nephropathy have not been observed. Epidemiologic investigation has not specifically examined the CIGA hypothesis for humans. Based on cancer bioassays, hormone manipulation studies, investigations in an alpha 2u-g-deficient strain of rat, and other laboratory data, an increased proliferative response caused by chemically induced cytotoxicity appears to play a role in the development of renal tubule tumors in male rats. Thus, it is reasonable to suggest that the renal effects induced in male rats by chemicals causing alpha 2u-g accumulation are unlikely to occur in humans. PMID:7686485

  11. Impact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation

    Science.gov (United States)

    Servais, Sophie; Menten-Dedoyart, Catherine; Beguin, Yves; Seidel, Laurence; Gothot, André; Daulne, Coline; Willems, Evelyne; Delens, Loïc; Humblet-Baron, Stéphanie; Hannon, Muriel; Baron, Frédéric

    2015-01-01

    Background Pre-transplant infusion of rabbit anti-T cell globulin (ATG) is increasingly used as prevention of graft-versus-host disease (GVHD) after allogeneic peripheral blood stem cell transplantation (PBSCT). However, the precise impact of pre-transplant ATG on immune recovery after PBSCT is still poorly documented. Methods In the current study, we compared immune recovery after myeloablative PBSCT in 65 patients who either received (n = 37) or did not (n = 28) pre-transplant ATG-Fresenius (ATG-F). Detailed phenotypes of circulating T, B, natural killer (NK) and invariant NKT (iNKT) cells were analyzed by multicolor flow cytometry at serial time-points from day 40 to day 365 after transplantation. Thymic function was also assessed by sjTREC quantification. Serious infectious events were collected up to 2 years post-transplantation. Results Pre-transplant ATG-F had a prolonged (for at least up to 1-year) and selective negative impact on the T-cell pool, while it did not impair the recovery of B, NK nor iNKT cells. Among T cells, ATG-F selectively compromised the recovery of naïve CD4+, central memory CD4+ and naïve CD8+ cells, while it spared effector memory T and regulatory T cells. Levels of sjTRECs were similar in both cohorts at 1-year after PBSCT, suggesting that ATG-F unlikely impaired thymopoiesis at long-term after PBSCT. Finally, the incidence and rate of serious infections were similar in both groups, while ATG-F patients had a lower incidence of grade II-IV acute graft-versus-host disease. Conclusions Pre-transplant ATG-F induces long-lasting modulation of the circulating T-cell pool after myeloablative PBSCT, that may participate in preventing graft-versus-host disease without deeply compromising anti-pathogen defenses. PMID:26098781

  12. Relatively high levels of serum adiponectin in obese women, a potential indicator of anti-inflammatory dysfunction: Relation to sex hormone-binding globulin

    Directory of Open Access Journals (Sweden)

    Altan Onat, Gülay Hergenç, Dursun Dursuno?lu, Zekeriya Küçükdurmaz, Serkan Bulur, Günay Can

    2008-01-01

    Full Text Available It is unclear whether serum adiponectin concentrations diminish linearly with increasing adiposity and, if not, which factors codetermine this association. These issues were investigated cross-sectionally in 1188 men and women, representative of middle-aged and elderly Turkish adults. Serum total adiponectin was assayed by ELISA. Serum adiponectin values in men, though declining significantly in transition from the bottom to the mid tertile of body mass index (BMI and waist circumference (WC, were similar in the two respective upper tertiles. In women, serum adiponectin concentrations were not significantly different in any tertile of these indices, were significantly correlated with BMI or WC within the low tertiles and not within the two higher tertiles. In a linear regression analysis for WC (or BMI in a subset of the sample in which serum sex hormone-binding globulin (SHBG was available and which additionally comprised adiponectin, fasting insulin and other confounders, only insulin and, in women SHBG, were significantly associated, but not adiponectin. In linear regression analyses for covariates of adiponectin in two models comprising 12 variables, insulin and SHBG concentrations were significantly associated in both genders though not BMI. Whereas in men HDL-cholesterol and CRP were covariates of adiponectin (both p<0.01, SHBG and apolipoprotein B positively associated in women (p<0.001, independent of BMI and fasting insulin levels. Conclusions: Relationship between excess adiposity and adiponectin levels is inconsistent in Turkish adults. Independently from obesity and hyperinsulinemia, serum adiponectin discloses significant relationship with inflammatory markers and HDL only in men, not in women in whom it is influenced by SHBG, with consequent attenuation of its anti-inflammatory activities.

  13. Comparison between spousal donor transplantation treated with anti-thymocyte globulin induction therapy and, living related donor transplantation treated with standard immunosuppression

    Directory of Open Access Journals (Sweden)

    Erkan Demir

    2014-01-01

    Full Text Available The worldwide shortage of organs available for transplantation has led to the use of living-unrelated kidney donors. In this context, spouses represent an important source of organ donors. We compared the allograft outcomes of spousal donor transplantation (SDT with anti-thymocyte globulin (ATG induction therapy and living related donor transplantation (LRDT with triple immonosuppression and basiliximab, in addition. Among the 335 living and deceased donor kidney transplantations performed between April 2001 and June 2010, there were 274 living donor kidney transplantations including 34 SDT and 240 LRDT. The minimum follow-up period was 36 months. All recipients of SDT received ATG (1.5 mg/kg induction therapy, which was stopped five to seven days after surgery. Maintenance immunosuppression included tacrolimus (TAC, mycophenolate mofetil (MMF and prednisolone. LRDT recipients received triple immunosuppressive protocol consisting of cyclosporine or TAC, MMF and prednisolone, in addition to basiliximab. There was a significant difference between the two groups in recipient age, while pre-operative duration on dialysis, recipient sex and donor age and sex were not significantly different. There was also a significant difference between the two groups in the number of human leukocyte antigen (HLA mismatches. The 1-, 3- and 5-year graft survival rates of SDT were 94.1%, 88.2% and 79.4%, respectively, and the frequency of acute rejection episodes was 5.8% (two cases. The 1-, 3- and 5-year graft survival rates of LRDT were 95.8%, 91.6% and 83.3%, respectively, with the frequency of acute rejection being 16.2%. The graft survival rates of SDT were as good as LRDT, while the acute rejection rates in SDT were lower than in LRDT, although the difference was not statistically different (P = 0.13.

  14. Comparison of long-term outcomes between children with aplastic anemia and refractory cytopenia of childhood who received immunosuppressive therapy with antithymocyte globulin and cyclosporine.

    Science.gov (United States)

    Hama, Asahito; Takahashi, Yoshiyuki; Muramatsu, Hideki; Ito, Masafumi; Narita, Atsushi; Kosaka, Yoshiyuki; Tsuchida, Masahiro; Kobayashi, Ryoji; Ito, Etsuro; Yabe, Hiromasa; Ohga, Shouichi; Ohara, Akira; Kojima, Seiji

    2015-11-01

    The 2008 World Health Organization classification proposed a new entity in childhood myelodysplastic syndrome, refractory cytopenia of childhood. However, it is unclear whether this morphological classification reflects clinical outcomes. We retrospectively reviewed bone marrow morphology in 186 children (median age 8 years; range 1-16 years) who were enrolled in the prospective study and received horse antithymocyte globulin and cyclosporine between July 1999 and November 2008. The median follow-up period was 87 months (range 1-146 months). Out of 186 patients, 62 (33%) were classified with aplastic anemia, 94 (49%) with refractory cytopenia of childhood, and 34 (18%) with refractory cytopenia with multilineage dysplasia. Aplastic anemia patients received granulocyte colony-stimulating factor more frequently and for longer durations than other patients (P<0.01). After six months, response rates to immunosuppressive therapy were not significantly different among the 3 groups. Acquisition of chromosomal abnormalities was observed in 5 patients with aplastic anemia, 4 patients with refractory cytopenia of childhood, and 3 patients with refractory cytopenia with multilineage dysplasia. Although the cumulative incidence of total clonal evolution at ten years was not significantly different among the 3 groups, the cumulative incidence of monosomy 7 development was significantly higher in aplastic anemia than in the other groups (P=0.02). Multivariate analysis revealed that only granulocyte colony-stimulating factor administration duration of 40 days or more was a significant risk factor for monosomy 7 development (P=0.02). These findings suggest that even the introduction of a strict morphological distinction from hypoplastic myelodysplastic syndrome cannot eradicate clonal evolution in children with aplastic anemia. PMID:26273061

  15. Serum levels of Sex Hormone Binding Globulin (SHBG) are not predictive of prostate cancer diagnosis and aggressiveness: results from an italian biopsy cohort

    Scientific Electronic Library Online (English)

    Cosimo, De Nunzio; Riccardo, Lombardo; Simone, Albisinni; Mauro, Gacci; Andrea, Tubaro.

    2013-12-01

    Full Text Available Purpose To explore the association between serum levels of Sex Hormone Binding Globulin (SHBG) and the risk of developing prostate cancer (PCa) as well as high grade disease in men undergoing prostate biopsy. Materials and Methods Between 2006 and 2012, we prospectively enrolled 740 patients with [...] no history of PCa undergoing prostate biopsy. Before biopsy general data of the patient DRE, PSA and BMI were recorded. The risk of detecting cancer and high grade cancer was assessed as a function of SHBG using crude and adjusted logistic regressions. Results Serum levels of SHBG were not associated with an increased risk of PCa or high grade disease. Age (OR 1.027 95% CI 1.003-1.052 p = 0.027), DRE (OR 3.391 95% CI 2.258-5.092 p = 0.000) and PSA (OR 1.078 95% CI 1.037-1.120 p = 0.000) were found to be independent predictors of prostate cancer risk. Age (OR 1.051 95% CI 1.009-1.095 p = 0.016), DRE (OR 2.519 95% CI 1.384-4.584 p = 0.000), BMI (OR 1.098 95% CI 1.011-1.193 p = 0.027) and PSA (OR 1.074 95% CI 1.014-1.137 p = 0.015) were found to be independent predictors of high grade disease. Conclusions In our cohort of patients, serum levels of SHBG are not predictive of PCa or high grade disease. According to our experience SHBG should not be considered a biomarker in PCa diagnosis neither a marker for high grade disease.

  16. Sex hormone-binding globulin and androgen levels in immigrant and British-born premenopausal British Pakistani women: evidence of early life influences?

    Science.gov (United States)

    Pollard, Tessa M; Unwin, Nigel C; Fischbacher, Colin M; Chamley, Jagdip K

    2006-01-01

    In women, raised insulin levels are associated with low sex hormone-binding globulin (SHBG) and high androgen levels, which are in turn linked to infertility. Since insulin resistance and hyperinsulinemia are major health problems for South Asians living in Western countries, we predicted that British Pakistani women would have low SHBG and raised androgen levels compared to European women. Given low birth weights in Pakistan, and known links between low birth weight and insulin resistance in later life, we also predicted that immigrant women born in Pakistan would have lower levels of SHBG and higher levels of androgens than British-born British Pakistani women. We assessed SHBG, testosterone, and the free androgen index (FAI) from a single serum sample taken on days 9-11 of the menstrual cycle from 20-40-year-old women living in the UK: 30 immigrants from Pakistan, 30 British-born British Pakistani women, and 25 British-born women of European origin. Age-adjusted analyses showed no significant differences in SHBG, testosterone, or FAI between British-born Pakistani and European-origin women. However, immigrant British Pakistani women had a significantly higher FAI than British-born British Pakistani women. Adjustment for body mass index, waist-to-hip ratio, and smoking status did not affect these results, but further adjustment for height, a marker of early environment, reduced the P-value for the difference in FAI between immigrant and British-born British Pakistani women to below significance. It is possible that the poorer early environment of immigrant British Pakistani women was at least partially responsible for their relatively high levels of free androgens. PMID:17039471

  17. PC-Cluster based Storage System Architecture for Cloud Storage

    OpenAIRE

    Yee, Tin Tin; Naing, Thinn Thu

    2011-01-01

    Design and architecture of cloud storage system plays a vital role in cloud computing infrastructure in order to improve the storage capacity as well as cost effectiveness. Usually cloud storage system provides users to efficient storage space with elasticity feature. One of the challenges of cloud storage system is difficult to balance the providing huge elastic capacity of storage and investment of expensive cost for it. In order to solve this issue in the cloud storage infrastructure, low ...

  18. Carbon Capture and Storage

    OpenAIRE

    Benson, S.M.; Bennaceur, K.; P. Cook; Davison, J.; de Coninck, H.; Farhat, K.; Ramirez, C A; Simbeck, D.; Surles, T.; Verma, P.; Wright, I.

    2012-01-01

    Emissions of carbon dioxide, the most important long-lived anthropogenic greenhouse gas, can be reduced by Carbon Capture and Storage (CCS). CCS involves the integration of four elements: CO 2 capture, compression of the CO2 from a gas to a liquid or a denser gas, transportation of pressurized CO 2 from the point of capture to the storage location, and isolation from the atmosphere by storage in deep underground rock formations. Considering full life-cycle emissions, CCS technology can red...

  19. Energy Storage: A Review

    OpenAIRE

    Himanshu Verma,; Prof. Jaimala Gambhir,; Sachin Goyal,

    2013-01-01

    Efficient and economic energy storage, if implemented in the current power infrastructure on a large scale, could bring about some of the greatest changes in the power industry in decades. Additionally, energy storage would improve the reliability and dynamic stability of the power system by providing stable, abundant energy reserves that require little ramp time and are less susceptible to varying fuel prices or shortages. Energy storage can shift the higher peak load to off-peak hours in or...

  20. Network Atached Storage

    OpenAIRE

    Griffee, Bill

    2013-01-01

    Digital distribution of media has grown increasingly important as an additional requirement is to have this media available to multiple users. Traditionally, servers have filled this role in companies by providing storage that can be accessed by other devices on a network, typically through file sharing or serving or via a storage area network, while providing other services as well. Network attached storage (NAS) systems expand upon traditional servers in that they are built specifically wit...

  1. Benchmarking Cloud Storage Systems

    OpenAIRE

    Wang,Xing

    2014-01-01

    With the rise of cloud computing, many cloud storage systems like Dropbox, Google Drive and Mega have been built to provide decentralized and reliable file storage. It is thus of prime importance to know their features, performance, and the best way to make use of them. In this context, we introduce BenchCloud, a tool designed as part of this thesis to conveniently and efficiently benchmark any cloud storage system.First, we provide a study of six commonly-used cloud storage systems to identi...

  2. Underground thermal energy storage

    CERN Document Server

    Lee, Kun Sang

    2012-01-01

    ""Underground Thermal Energy Storage"" (UTES) provide us with a flexible tool to combat global warming through conserving energy while utilizing natural renewable energy resources. Primarily, they act as a buffer to balance fluctuations in supply and demand of low temperature thermal energy. ""Underground Thermal Energy Storage"" provides an comprehensive introduction to the extensively-used energy storage method. ""Underground Thermal Energy Storage"" gives a general overview of UTES from basic concepts and classifications to operation regimes. As well as discussing general procedures for des

  3. Monitored retrievable storage (MRS) facility surge storage systems

    International Nuclear Information System (INIS)

    The Monitored Retrievable Storage (MRS) Facility includes surge storage for canistered commercial spent fuels and associated wastes. This storage is provided by air-cooled vaults and passive-cooled concrete storage casks. This paper, which was sponsored by the U.S. Department of Energy, discusses the design and analysis for these storage systems

  4. The storage-ring Free Electron Laser. 10

    International Nuclear Information System (INIS)

    The opportunities and problems are described with using a storage ring as the electron beam source for free-electron lasers. Several unique aspects of the storage-ring laser include the optimization, the power scaling, and the configuration options. Extensive examples are taken from various storage-ring laser experiments. (author). 78 refs., 13 figs., 4 tabs

  5. A new storage-ring light source

    Energy Technology Data Exchange (ETDEWEB)

    Chao, Alex [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2015-06-01

    A recently proposed technique in storage ring accelerators is applied to provide potential high-power sources of photon radiation. The technique is based on the steady-state microbunching (SSMB) mechanism. As examples of this application, one may consider a high-power DUV photon source for research in atomic and molecular physics or a high-power EUV radiation source for industrial lithography. A less challenging proof-of-principle test to produce IR radiation using an existing storage ring is also considered.

  6. A Survey on Cloud Storage

    Directory of Open Access Journals (Sweden)

    Jiehui Ju

    2011-08-01

    Full Text Available Cloud storage is a new concept come into being simultaneously with cloud computing, and can be divided into public cloud storage, private cloud storage and hybrid cloud storage. This article gives a quick introduction to cloud storage. It covers the key technologies in Cloud Computing and Cloud Storage. Google GFS massive data storage system and the popular open source Hadoop HDFS were detailed introduced to analyze the principle of Cloud Storage technology. As an important technology area and research direction, cloud storage is becoming a hot research for both academia and industry session. The future valuable research works were summarized at the end.

  7. Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

    Directory of Open Access Journals (Sweden)

    Agnieszka I. Piotrowicz-Cie?lak

    2011-04-01

    Full Text Available Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L. cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC. Seed storage at room temperature reduced viability (to 2% and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered.

  8. Pulsed electrical energy storage systems

    International Nuclear Information System (INIS)

    The starting point of any pulsed power setup is the energy storage system. This article discusses the characteristics of various energy storage devices useful for high power pulse discharges. The energy storage systems discussed are capacitative storage such as capacitor and Marx bank, inductive storage systems and explosively driven flux compression generators. (author)

  9. Wind-energy storage

    Science.gov (United States)

    Gordon, L. H.

    1980-01-01

    Program SIMWEST can model wind energy storage system using any combination of five types of storage: pumped hydro, battery, thermal, flywheel, and pneumatic. Program is tool to aid design of optional system for given application with realistic simulation for further evaluation and verification.

  10. Energy storage capacitors

    Energy Technology Data Exchange (ETDEWEB)

    Sarjeant, W.J.

    1984-01-01

    The properties of capacitors are reviewed in general, including dielectrics, induced polarization, and permanent polarization. Then capacitance characteristics are discussed and modelled. These include temperature range, voltage, equivalent series resistance, capacitive reactance, impedance, dissipation factor, humidity and frequency effects, storage temperature and time, and lifetime. Applications of energy storage capacitors are then discussed. (LEW)

  11. Reversible storage of hydrogen

    International Nuclear Information System (INIS)

    In the framework of the reversible hydrogen storage, this laboratory is studying the hydrogen storage and more specifically reversible metallic hydrides. As a light, high mass capacity and economical element, the magnesium seems to be a good solution. The authors presents the operating of this fuel cell and the first results. (A.L.B.)

  12. Mass Storage Systems.

    Science.gov (United States)

    Ranade, Sanjay; Schraeder, Jeff

    1991-01-01

    Presents an overview of the mass storage market and discusses mass storage systems as part of computer networks. Systems for personal computers, workstations, minicomputers, and mainframe computers are described; file servers are explained; system integration issues are raised; and future possibilities are suggested. (LRW)

  13. Open systems storage platforms

    Science.gov (United States)

    Collins, Kirby

    1992-01-01

    The building blocks for an open storage system includes a system platform, a selection of storage devices and interfaces, system software, and storage applications CONVEX storage systems are based on the DS Series Data Server systems. These systems are a variant of the C3200 supercomputer with expanded I/O capabilities. These systems support a variety of medium and high speed interfaces to networks and peripherals. System software is provided in the form of ConvexOS, a POSIX compliant derivative of 4.3BSD UNIX. Storage applications include products such as UNITREE and EMASS. With the DS Series of storage systems, Convex has developed a set of products which provide open system solutions for storage management applications. The systems are highly modular, assembled from off the shelf components with industry standard interfaces. The C Series system architecture provides a stable base, with the performance and reliability of a general purpose platform. This combination of a proven system architecture with a variety of choices in peripherals and application software allows wide flexibility in configurations, and delivers the benefits of open systems to the mass storage world.

  14. Storage of radioactive materials

    International Nuclear Information System (INIS)

    The standard applies to the storage of radioactive materials, excluding nuclear fuels, ('other radioactive materials' within the purview of sec. 2, sub-sec. (1), no. 1 AtG), as e.g. for applications in industry or medicine. The standard is also applicable to storage of nuclear fuels up to the tenth of the value granted general exemption from licensing requirements, but not from notification, excluding UF6 (sec. 4 Radiation Protection Ordinance) and interim storage of radioactive wastes in accordance with sec. 9a AtG, ultimate disposal and storage being excluded. The standard defines safety requirements within the purview of sections 6 and 74 Radiation Protection Ordinance as of October 13, 1976, as far as the storage of 'other radioactive materials' is concerned. (orig./HSCH)

  15. Plutonium storage criteria

    Energy Technology Data Exchange (ETDEWEB)

    Chung, D. [Scientech, Inc., Germantown, MD (United States); Ascanio, X. [Dept. of Energy, Germantown, MD (United States)

    1996-05-01

    The Department of Energy has issued a technical standard for long-term (>50 years) storage and will soon issue a criteria document for interim (<20 years) storage of plutonium materials. The long-term technical standard, {open_quotes}Criteria for Safe Storage of Plutonium Metals and Oxides,{close_quotes} addresses the requirements for storing metals and oxides with greater than 50 wt % plutonium. It calls for a standardized package that meets both off-site transportation requirements, as well as remote handling requirements from future storage facilities. The interim criteria document, {open_quotes}Criteria for Interim Safe Storage of Plutonium-Bearing Solid Materials{close_quotes}, addresses requirements for storing materials with less than 50 wt% plutonium. The interim criteria document assumes the materials will be stored on existing sites, and existing facilities and equipment will be used for repackaging to improve the margin of safety.

  16. Energy storage; Energiespeicher

    Energy Technology Data Exchange (ETDEWEB)

    Baufume, Sylvestre; Hennings, Wilfried; Kuckshinrichs, Wilhelm; Linssen, Jochen; Markewitz, Peter; Strenzel, Peter; Voegele, Stefan [Forschungszentrum Juelich GmbH (DE). Inst. fuer Energieforschung (IEF), Systemforschung und Technologische Entwicklung (IEF-STE)

    2012-07-01

    The accelerated system integration of renewable energy resources and the increase of energy efficiency are two major pillars of the current energy plan of the Federal Government. With this, the development of energy storage is seen as a central key element. The current focus primarily is on the storage of electricity. Right now, major research efforts are being made for the storage of excess quantities of electricity and to compensate for seasonal fluctuations in power generation from renewable energy sources. Possible approaches are examined. Even today, natural gas storage facilities play a major role in the power generation because of seasonal fluctuations of the energy demand can be compensated. In particular, the storage of electricity in the form of chemical energy (hydrogen or methane) currently is being discussed. This power-to-gas approach results in a combination of electricity and gas network infrastructures.

  17. Seasonal thermal energy storage

    Science.gov (United States)

    Allen, R. D.; Kannberg, L. D.; Raymond, J. R.

    1984-05-01

    Seasonal thermal energy storage (STES) using heat or cold available from surplus, waste, climatic, or cogeneration sources show great promise to reduce peak demand, reduce electric utility load problems, and contribute to establishing favorable economics for district heating and cooling systems. Heated and chilled water can be injected, stored, and recovered from aquifers. Geologic materials are good thermal insulators, and potentially suitable aquifers are distributed throughout the United States. Potential energy sources for use in an aquifer thermal energy storage system include solar heat, power plant cogeneration, winter chill, and industrial waste heat source. Topics covered include: (1) the U.S. Department of Energy seasonal thermal energy storage program; (2) aquifer thermal energy storage technology; (3) alternative STES technology; (4) foreign studies in seasonal thermal energy storage; and (5) economic assessment.

  18. Electricity Storage. Technology Brief

    Energy Technology Data Exchange (ETDEWEB)

    Simbolotti, G. [Italian National Agency for New Technologies, Energy and Sustainable Economic Development ENEA, Rome (Italy); Kempener, R. [International Renewable Energy Agency IRENA, Bonn (Germany)

    2012-04-15

    Electricity storage is a key technology for electricity systems with a high share of renewables as it allows electricity to be generated when renewable sources (i.e. wind, sunlight) are available and to be consumed on demand. It is expected that the increasing price of fossil fuels and peak-load electricity and the growing share of renewables will result in electricity storage to grow rapidly and become more cost effective. However, electricity storage is technically challenging because electricity can only be stored after conversion into other forms of energy, and this involves expensive equipment and energy losses. At present, the only commercial storage option is pumped hydro power where surplus electricity (e.g. electricity produced overnight by base-load coal or nuclear power) is used to pump water from a lower to an upper reservoir. The stored energy is then used to produce hydropower during daily high-demand periods. Pumped hydro plants are large-scale storage systems with a typical efficiency between 70% and 80%, which means that a quarter of the energy is lost in the process. Other storage technologies with different characteristics (i.e. storage process and capacity, conversion back to electricity and response to power demand, energy losses and costs) are currently in demonstration or pre-commercial stages and discussed in this brief report: Compressed air energy storage (CAES) systems, Flywheels; Electrical batteries; Supercapacitors; Superconducting magnetic storage; and Thermal energy storage. No single electricity storage technology scores high in all dimensions. The technology of choice often depends on the size of the system, the specific service, the electricity sources and the marginal cost of peak electricity. Pumped hydro currently accounts for 95% of the global storage capacity and still offers a considerable expansion potential but does not suit residential or small-size applications. CAES expansion is limited due to the lack of suitable natural storage sites. Electrical batteries have a large potential with a number of new materials and technologies under development to improve performance and reduce costs. Heat storage is practical in CSP plants. The choice between large-scale storage facilities and small-scale distributed storage depends on the geography and demography of the country, the existing grid and the type and scale of renewable technologies entering the market. While the energy storage market is quickly evolving and expected to increase 20-fold between 2010 and 2020, many electricity storage technologies are under development and need policy support for further commercial deployment. Electricity storage considerations should be an integral part of any plans for electric grid expansion or transformation of the electricity system. Storage also offers key synergies with grid interconnection and methods to smooth the variability of electricity demand (demand side management)

  19. The comparison of the measurement of human serum thyroid globulin auto-antibodies and thyroid peroxidase auto-antibodies by CLIA and RIA

    International Nuclear Information System (INIS)

    The serum levels of thyroid globulin auto-antibodies (Anti-TgAb) and thyroid peroxidase auto-antibodies (Anti-TpoAb) in 37 patients with hyperthyroidism, 30 with chronic lymphocytic thyroidism, 36 with other endocrine diseases and 35 healthy persons were determined by chemiluminescence immunoassay (CLIA) and RIA, and the results were compared with each other. The results showed: (1) The within-batch CVs of CLIA and RIA were 3.0% and 10.0%, and the between-batch CVs were 3.9% and 15.0%, respectively; (2) The levels of serum anti-TgAb and Anti-TpoAb in healthy people were 25.9±9.6 U/mL and 31.4±6.7U/mL by CLIA, while those were 11.2±2.8% and 8.7±3.0% by RIA; (3) The levels of serum anti-TgAb and Anti-TpoAb of chronic lymphocytic thyroidism were 292.6±334.1U/mL and 5043.3±3196.1U/mL (n=17) by CLIA, while those were 56.4±11.2% (n=21) and 35.4±6.9% (n=21) by RIA. It showed that levels of anti-TgAb and Anti-TpoAb of chronic lymphocytic thyroidism were much higher than that in healthy people by CLIA (P<0.001). Furthermore, the levels of Anti-TpoAb were more than 100 times of that in health people by CLIA and the specificity of Anti-TpoAb was higher; (4) The levels of anti-TgAb and Anti-TpoAb in hyperthyroid patients were 202.3±506.3U/mL and 452.9±645.8U/mL by CLIA, while those were 28.8±20.0% and 22.6±14.2% by RIA. The levels of Anti-TgAb and Anti-TpoAb in patients with other endocrine disease were 28.7±15.0U/mL and 58.8±45.7U/mL by CLIA, while those were 10.2±13.3% and 7.9±7.7% by RIA; (5) These is a significant correlation between the two methods: Anti TgAb r=0.695; and Anti-TpoAb r=0.489. This results show that it may be more sensitive and specific test of Anti-TpoAb with CLIA than with RIA. CLIA should be used as a powerful diagnostic tool in clinical because it is simple, quick and without radioisotope

  20. Hepatitis C virus genotypes and the influence of the induction of immunosuppression with anti-thymocyte globulin (ATG) on chronic hepatitis in renal graft recipients.

    Science.gov (United States)

    Rodrigues, A; Pinho, L; Lobato, L; Queirós, A; Castro, R; Daniel, M; Dias, L; Henriques, A C; Sarmento, A; Guimarães, S

    1998-01-01

    Hepatitis C virus (HCV) exhibits a dramatic genetic variability and several mechanisms of immunological response are unable to control hepatic and extrahepatic replication. Genotype 1 b is associated with more severe clinical manifestations and is less responsive to interferon. In addition, we have reported an increase of HCV RNA viral load after renal transplantation. Anti-thymocyte globulin (ATG) is supposed to increase viral replication and liver dysfunction in chronically infected renal graft recipients. We evaluated the genotype profile in HCV+ patients of our Renal Transplant Unit and studied the effects of ATG, as part of the induction of immunosuppression, on viral load and liver enzymes abnormalities. From 726 renal graft recipients, 104 patients, with a mean follow up of 3.9 +/- 2.9 years, were anti-HCV+ by ELISA II. HCV RNA was measured by quantitative PCR. We correlated the viral load and biochemical liver parameters with genotype, exposure to ATG as induction therapy, early acute rejection episode and the duration of infection. Of the 81 patients tested, 72% were viraemic and genotype 1 b was the predominant viral strain (66%). The majority of these patients (65%) were coinfected by two or more strains. There was no correlation between HCV RNA blood levels and liver enzymes. We did not find higher viral load with genotype 1 b infection (68 +/- 88 mEq/ml vs 75.8 +/- 123 mEq/ml in the others) nor with ATG induction therapy (43.5 +/- 71.3 mEq/ml vs 64.1 +/- 110.5 mEq/ml). Early acute rejection and longer follow up were not associated with higher levels of HCV RNA. The biochemical liver profile showed no relationship with the variables studied. We concluded that genotype 1 b is the predominant strain in our HCV+ population and there is a great prevalence of coinfection with several genotypes. Our results did not confirm a deleterious effect of the use of ATG as induction therapy in these HCV-infected patients. Prospective randomised studies with liver biopsy evaluation are needed to answer more fully the remaining questions about the best immunosuppressive therapy in renal graft recipients with chronic HCV infection. PMID:9664959

  1. Testosterone, Sex Hormone-Binding Globulin and the Metabolic Syndrome in Men: An Individual Participant Data Meta-Analysis of Observational Studies

    Science.gov (United States)

    Brand, Judith S.; Rovers, Maroeska M.; Yeap, Bu B.; Schneider, Harald J.; Tuomainen, Tomi-Pekka; Haring, Robin; Corona, Giovanni; Onat, Altan; Maggio, Marcello; Bouchard, Claude; Tong, Peter C. Y.; Chen, Richard Y. T.; Akishita, Masahiro; Gietema, Jourik A.; Gannagé-Yared, Marie-Hélène; Undén, Anna-Lena; Goncharov, Nicolai P.; Kumanov, Philip; Chubb, S. A. Paul; Almeida, Osvaldo P.; Wittchen, Hans-Ulrich; Klotsche, Jens; Wallaschofski, Henri; Völzke, Henry; Kauhanen, Jussi; Salonen, Jukka T.; Ferrucci, Luigi; van der Schouw, Yvonne T.

    2014-01-01

    Background Low total testosterone (TT) and sex hormone-binding globulin (SHBG) concentrations have been associated with the metabolic syndrome (MetS) in men, but the reported strength of association varies considerably. Objectives We aimed to investigate whether associations differ across specific subgroups (according to age and body mass index (BMI)) and individual MetS components. Data sources Two previously published meta-analyses including an updated systematic search in PubMed and EMBASE. Study Eligibility Criteria Cross-sectional or prospective observational studies with data on TT and/or SHBG concentrations in combination with MetS in men. Methods We conducted an individual participant data meta-analysis of 20 observational studies. Mixed effects models were used to assess cross-sectional and prospective associations of TT, SHBG and free testosterone (FT) with MetS and its individual components. Multivariable adjusted odds ratios (ORs) and hazard ratios (HRs) were calculated and effect modification by age and BMI was studied. Results Men with low concentrations of TT, SHBG or FT were more likely to have prevalent MetS (ORs per quartile decrease were 1.69 (95% CI 1.60-1.77), 1.73 (95% CI 1.62-1.85) and 1.46 (95% CI 1.36-1.57) for TT, SHBG and FT, respectively) and incident MetS (HRs per quartile decrease were 1.25 (95% CI 1.16-1.36), 1.44 (95% 1.30-1.60) and 1.14 (95% 1.01-1.28) for TT, SHBG and FT, respectively). Overall, the magnitude of associations was largest in non-overweight men and varied across individual components: stronger associations were observed with hypertriglyceridemia, abdominal obesity and hyperglycaemia and associations were weakest for hypertension. Conclusions Associations of testosterone and SHBG with MetS vary according to BMI and individual MetS components. These findings provide further insights into the pathophysiological mechanisms linking low testosterone and SHBG concentrations to cardiometabolic risk. PMID:25019163

  2. Electricity storage using a thermal storage scheme

    Science.gov (United States)

    White, Alexander

    2015-01-01

    The increasing use of renewable energy technologies for electricity generation, many of which have an unpredictably intermittent nature, will inevitably lead to a greater demand for large-scale electricity storage schemes. For example, the expanding fraction of electricity produced by wind turbines will require either backup or storage capacity to cover extended periods of wind lull. This paper describes a recently proposed storage scheme, referred to here as Pumped Thermal Storage (PTS), and which is based on "sensible heat" storage in large thermal reservoirs. During the charging phase, the system effectively operates as a high temperature-ratio heat pump, extracting heat from a cold reservoir and delivering heat to a hot one. In the discharge phase the processes are reversed and it operates as a heat engine. The round-trip efficiency is limited only by process irreversibilities (as opposed to Second Law limitations on the coefficient of performance and the thermal efficiency of the heat pump and heat engine respectively). PTS is currently being developed in both France and England. In both cases, the schemes operate on the Joule-Brayton (gas turbine) cycle, using argon as the working fluid. However, the French scheme proposes the use of turbomachinery for compression and expansion, whereas for that being developed in England reciprocating devices are proposed. The current paper focuses on the impact of the various process irreversibilities on the thermodynamic round-trip efficiency of the scheme. Consideration is given to compression and expansion losses and pressure losses (in pipe-work, valves and thermal reservoirs); heat transfer related irreversibility in the thermal reservoirs is discussed but not included in the analysis. Results are presented demonstrating how the various loss parameters and operating conditions influence the overall performance.

  3. Electricity storage using a thermal storage scheme

    Energy Technology Data Exchange (ETDEWEB)

    White, Alexander, E-mail: ajw36@cam.ac.uk [Hopkinson Laboratory, Cambridge University Engineering Department, Trumpington Street, Cambridge. CB2 1PZ (United Kingdom)

    2015-01-22

    The increasing use of renewable energy technologies for electricity generation, many of which have an unpredictably intermittent nature, will inevitably lead to a greater demand for large-scale electricity storage schemes. For example, the expanding fraction of electricity produced by wind turbines will require either backup or storage capacity to cover extended periods of wind lull. This paper describes a recently proposed storage scheme, referred to here as Pumped Thermal Storage (PTS), and which is based on “sensible heat” storage in large thermal reservoirs. During the charging phase, the system effectively operates as a high temperature-ratio heat pump, extracting heat from a cold reservoir and delivering heat to a hot one. In the discharge phase the processes are reversed and it operates as a heat engine. The round-trip efficiency is limited only by process irreversibilities (as opposed to Second Law limitations on the coefficient of performance and the thermal efficiency of the heat pump and heat engine respectively). PTS is currently being developed in both France and England. In both cases, the schemes operate on the Joule-Brayton (gas turbine) cycle, using argon as the working fluid. However, the French scheme proposes the use of turbomachinery for compression and expansion, whereas for that being developed in England reciprocating devices are proposed. The current paper focuses on the impact of the various process irreversibilities on the thermodynamic round-trip efficiency of the scheme. Consideration is given to compression and expansion losses and pressure losses (in pipe-work, valves and thermal reservoirs); heat transfer related irreversibility in the thermal reservoirs is discussed but not included in the analysis. Results are presented demonstrating how the various loss parameters and operating conditions influence the overall performance.

  4. Electricity storage using a thermal storage scheme

    International Nuclear Information System (INIS)

    The increasing use of renewable energy technologies for electricity generation, many of which have an unpredictably intermittent nature, will inevitably lead to a greater demand for large-scale electricity storage schemes. For example, the expanding fraction of electricity produced by wind turbines will require either backup or storage capacity to cover extended periods of wind lull. This paper describes a recently proposed storage scheme, referred to here as Pumped Thermal Storage (PTS), and which is based on “sensible heat” storage in large thermal reservoirs. During the charging phase, the system effectively operates as a high temperature-ratio heat pump, extracting heat from a cold reservoir and delivering heat to a hot one. In the discharge phase the processes are reversed and it operates as a heat engine. The round-trip efficiency is limited only by process irreversibilities (as opposed to Second Law limitations on the coefficient of performance and the thermal efficiency of the heat pump and heat engine respectively). PTS is currently being developed in both France and England. In both cases, the schemes operate on the Joule-Brayton (gas turbine) cycle, using argon as the working fluid. However, the French scheme proposes the use of turbomachinery for compression and expansion, whereas for that being developed in England reciprocating devices are proposed. The current paper focuses on the impact of the various process irreversibilities on the thermodynamic round-trip efficiency of the scheme. Consideration is given to compression and expansion losses and pressure losses (in pipe-work, valves and thermal reservoirs); heat transfer related irreversibility in the thermal reservoirs is discussed but not included in the analysis. Results are presented demonstrating how the various loss parameters and operating conditions influence the overall performance

  5. Cytomegalovirus Immune Globulin Intravenous Injection

    Science.gov (United States)

    ... body's natural response to infection after a kidney transplant. The drug will be added to an intravenous fluid that will drip through a needle or catheter placed in your vein for 2 to 4 hours one ... transplant. This medication is sometimes prescribed for other uses; ...

  6. Aboveground storage tank regulations

    International Nuclear Information System (INIS)

    There are critical differences between the potential for environmental impact of aboveground and underground oil storage. For example, while leaks from underground storage tanks (USTs) seep into soil or aquifers, the concern with aboveground storage tanks (ASTs) is that an overfill or tank rupture can cause product to escape into a navigable stream and immediately create an oil spill pollution incident. The US Environmental Protection Agency (EPA) has very distinct programs outlining regulation parameters for each type of storage, including source of authority, regulatory cutoffs and exclusions, definitions, prevention and response requirements, and penalties, etc. Engineers considering changing or recommending a change in type of storage, particularly from a UST to an AST, need to be aware of existing federal regulations. UST regulation, administered primarily by the states, falls under EPA Regulation 40 CFR 280. EPA's underground storage tank program, which began in 1988, and the individual state programs that have evolved since then, provide generally accepted benchmarks for safe, reliable underground storage of petroleum and hazardous liquid products

  7. Aflatoxins & Safe Storage

    Directory of Open Access Journals (Sweden)

    PhilippeVillers

    2014-04-01

    Full Text Available The paper examines both field experience and research on the prevention of the exponential growth of aflatoxins during multi-month post harvest storage in hot, humid countries. The approach described is the application of modern safe storage methods using flexible, Ultra Hermetic™ structures that create an unbreatheable atmosphere through insect and microorganism respiration alone, without use of chemicals, fumigants, or pumps. Laboratory and field data are cited and specific examples are given describing the uses of Ultra Hermetic storage to prevent the growth of aflatoxins with their significant public health consequences. Also discussed is the presently limited quantitative information on the relative occurrence of excessive levels of aflatoxin (>20 ppb before versus after multi-month storage of such crops as maize, rice and peanuts when under high humidity, high temperature conditions and, consequently, the need for further research to determine the frequency at which excessive aflatoxin levels are reached in the field versus after months of post-harvest storage. The significant work being done to reduce aflatoxin levels in the field is mentioned, as well as its probable implications on post harvest storage. Also described is why, with some crops such as peanuts, using Ultra Hermetic storage may require injection of carbon dioxide or use of an oxygen absorber as an accelerant. The case of peanuts is discussed and experimental data is described.

  8. Ultrafine hydrogen storage powders

    Science.gov (United States)

    Anderson, Iver E. (Ames, IA); Ellis, Timothy W. (Doylestown, PA); Pecharsky, Vitalij K. (Ames, IA); Ting, Jason (Ames, IA); Terpstra, Robert (Ames, IA); Bowman, Robert C. (La Mesa, CA); Witham, Charles K. (Pasadena, CA); Fultz, Brent T. (Pasadena, CA); Bugga, Ratnakumar V. (Arcadia, CA)

    2000-06-13

    A method of making hydrogen storage powder resistant to fracture in service involves forming a melt having the appropriate composition for the hydrogen storage material, such, for example, LaNi.sub.5 and other AB.sub.5 type materials and AB.sub.5+x materials, where x is from about -2.5 to about +2.5, including x=0, and the melt is gas atomized under conditions of melt temperature and atomizing gas pressure to form generally spherical powder particles. The hydrogen storage powder exhibits improved chemcial homogeneity as a result of rapid solidfication from the melt and small particle size that is more resistant to microcracking during hydrogen absorption/desorption cycling. A hydrogen storage component, such as an electrode for a battery or electrochemical fuel cell, made from the gas atomized hydrogen storage material is resistant to hydrogen degradation upon hydrogen absorption/desorption that occurs for example, during charging/discharging of a battery. Such hydrogen storage components can be made by consolidating and optionally sintering the gas atomized hydrogen storage powder or alternately by shaping the gas atomized powder and a suitable binder to a desired configuration in a mold or die.

  9. Superconducting magnetic energy storage

    International Nuclear Information System (INIS)

    After a brief review of the reasons for and forms of secondary energy storage and of the elements and history of inductive or magnetic storage, the four distinct areas in which superconducting magnetic energy storage can be applied are discussed. Differences in energy transfer times place different requirements on the storage coil, on the switch or transfer element, and on the energy losses in the superconductor. A report on designs and experiments in one of these areas with 2 to 300kJ units, and on the analysis and plans for an installation that is to provide 250kJ of plasma compression energy for the theta-pitch controlled thermonuclear fusion test reactor is given. Those elements of inductive storage that need further development before a theta-pitch fusion reactor can become economically competitive are pointed out. The size and costs of the energy storage components of these systems compared with similar and with larger inductive storage systems that are to interact reversibly with electric utility networks. (author)

  10. Spent-fuel-storage alternatives

    International Nuclear Information System (INIS)

    The Spent Fuel Storage Alternatives meeting was a technical forum in which 37 experts from 12 states discussed storage alternatives that are available or are under development. The subject matter was divided into the following five areas: techniques for increasing fuel storage density; dry storage of spent fuel; fuel characterization and conditioning; fuel storage operating experience; and storage and transport economics. Nineteen of the 21 papers which were presented at this meeting are included in this Proceedings. These have been abstracted and indexed

  11. Gas Storage Technology Consortium

    Energy Technology Data Exchange (ETDEWEB)

    Joel L. Morrison; Sharon L. Elder

    2007-06-30

    Gas storage is a critical element in the natural gas industry. Producers, transmission and distribution companies, marketers, and end users all benefit directly from the load balancing function of storage. The unbundling process has fundamentally changed the way storage is used and valued. As an unbundled service, the value of storage is being recovered at rates that reflect its value. Moreover, the marketplace has differentiated between various types of storage services and has increasingly rewarded flexibility, safety, and reliability. The size of the natural gas market has increased and is projected to continue to increase towards 30 trillion cubic feet over the next 10 to 15 years. Much of this increase is projected to come from electric generation, particularly peaking units. Gas storage, particularly the flexible services that are most suited to electric loads, is crucial in meeting the needs of these new markets. To address the gas storage needs of the natural gas industry, an industry-driven consortium was created--the Gas Storage Technology Consortium (GSTC). The objective of the GSTC is to provide a means to accomplish industry-driven research and development designed to enhance the operational flexibility and deliverability of the nation's gas storage system, and provide a cost-effective, safe, and reliable supply of natural gas to meet domestic demand. This report addresses the activities for the quarterly period of April 1, 2007 through June 30, 2007. Key activities during this time period included: (1) Organizing and hosting the 2007 GSTC Spring Meeting; (2) Identifying the 2007 GSTC projects, issuing award or declination letters, and begin drafting subcontracts; (3) 2007 project mentoring teams identified; (4) New NETL Project Manager; (5) Preliminary planning for the 2007 GSTC Fall Meeting; (6) Collecting and compiling the 2005 GSTC project final reports; and (7) Outreach and communications.

  12. Monitored retrievable storage design

    International Nuclear Information System (INIS)

    The Nuclear Waste Policy Act of 1982 (NWPA) established a national policy for the safe storage and disposal of spent nuclear fuel and high-level radioactive waste. The NWPA requires that DOE... ''submit a proposal to Congress on the need for and feasibility of one or more Monitored Retrievable Storage (MRS) Facilities''... In subsequent evaluations of the commercial nuclear waste management system, DOE has identified important advantages in providing an MRS Facility as an integral part of the total system. The integral MRS Facility serves as an independent, centralized spent nuclear fuel and high-level waste handling and packaging facility with a safe temporary storage capacity

  13. Tiered Storage For LHC

    CERN Document Server

    CERN. Geneva; Hanushevsky, Andrew

    2012-01-01

    For more than a year, the ATLAS Western Tier 2 (WT2) at SLAC National Accelerator has been successfully operating a two tiered storage system based on Xrootd's flexible cross-cluster data placement framework, the File Residency Manager. The architecture allows WT2 to provide both, high performance storage at the higher tier to ATLAS analysis jobs, as well as large, low cost disk capacity at the lower tier. Data automatically moves between the two storage tiers based on the needs of analysis jobs and is completely transparent to the jobs.

  14. Memory mass storage

    Energy Technology Data Exchange (ETDEWEB)

    Campardo, Giovanni [Numonyx, Agrate Brianza Milano (Italy); Tiziani, Federico; Iaculo, Massimo (eds.) [Micron, Arzano Napoli (Italy)

    2011-07-01

    Memory Mass Storage describes the fundamental storage technologies, like Semiconductor, Magnetic, Optical and Uncommon, detailing the main technical characteristics of the storage devices. It deals not only with semiconductor and hard disk memory, but also with different ways to manufacture and assembly them, and with their application to meet market requirements. It also provides an introduction to the epistemological issues arising in defining the process of remembering, as well as an overview on human memory, and an interesting excursus about biological memories and their organization, to better understand how the best memory we have, our brain, is able to imagine and design memory. (orig.)

  15. Interim storage cask design

    International Nuclear Information System (INIS)

    General Atomics (GA) has designed and is currently fabricating the Interim Storage Cask (ISC) for Westinghouse Hanford Company (WHC). The ISC is a dry storage concrete and steel cask that will be used to safely store a Core Component Container (CCC) with Fast Flux Test Facility (FFTF) spent fuel assemblies or fuel pin containers for a period of up to 50 years at the U.S. Department of Energy (DOE) Hanford site. The construction materials make the ISC a cost-effective storage cask

  16. Memory mass storage

    CERN Document Server

    Campardo, Giovanni; Iaculo, Massimo

    2011-01-01

    Memory Mass Storage describes the fundamental storage technologies, like Semiconductor, Magnetic, Optical and Uncommon, detailing the main technical characteristics of the storage devices. It deals not only with semiconductor and hard disk memory, but also with different ways to manufacture and assembly them, and with their application to meet market requirements. It also provides an introduction to the epistemological issues arising in defining the process of remembering, as well as an overview on human memory, and an interesting excursus about biological memories and their organization, to b

  17. On Storage Operators

    OpenAIRE

    Nour, Karim

    2009-01-01

    In 1990 Krivine introduced the notion of storage operators. They are $\\lambda$-terms which simulate call-by-value in the call-by-name strategy. Krivine has shown that there is a very simple type in the AF2 type system for storage operators using G\\"odel translation from classical to intuitionistic logic. Parigot and Krivine have shown that storage operators play an important tool in classical logic. In this paper, we present a synthesis of various results on this subject.

  18. Storage-ring FEL for the vuv

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, J.M.; Bisognano, J.J.; Garren, A.A.; Halbach, K.; Kim, K.J.; Sah, R.C.

    1984-09-01

    A free-electron laser for the vuv operating in a storage ring requires an electron beam of high density and low energy spread and a short wavelength, narrow-gap undulator. These conditions tend to produce longitudinal and transverse beam instabilities, excessive beam growth through multiple intrabeam scattering, and a short gas-scattering lifetime. Passing the beam only occasionally through the undulator in a by-pass straight section, as proposed by Murphy and Pellegrini, allows operation in a high-gain, single-pass mode and a long gas-scattering lifetime. Several storage ring designs have been considered to see how best to satisfy the several requirements. Each features a by-pass, a low-emittance lattice, and built-in wigglers for enhanced damping to counteract the intra-beam scattering. 15 references, 3 figures, 2 tables.

  19. Storage-ring FEL for the vuv

    International Nuclear Information System (INIS)

    A free-electron laser for the vuv operating in a storage ring requires an electron beam of high density and low energy spread and a short wavelength, narrow-gap undulator. These conditions tend to produce longitudinal and transverse beam instabilities, excessive beam growth through multiple intrabeam scattering, and a short gas-scattering lifetime. Passing the beam only occasionally through the undulator in a by-pass straight section, as proposed by Murphy and Pellegrini, allows operation in a high-gain, single-pass mode and a long gas-scattering lifetime. Several storage ring designs have been considered to see how best to satisfy the several requirements. Each features a by-pass, a low-emittance lattice, and built-in wigglers for enhanced damping to counteract the intra-beam scattering. 15 references, 3 figures, 2 tables

  20. Storage Gage Precipitation Observations

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A storage gage is a precipitation gage that requires reading and maintenance only monthly or seasonal intervals. This library includes reports from such gages,...

  1. Wet storage integrity update

    International Nuclear Information System (INIS)

    This report includes information from various studies performed under the Wet Storage Task of the Spent Fuel Integrity Project of the Commercial Spent Fuel Management (CSFM) Program at Pacific Northwest Laboratory. An overview of recent developments in the technology of wet storage of spent water reactor fuel is presented. Licensee Event Reports pertaining to spent fuel pools and the associated performance of spent fuel and storage components during wet storage are discussed. The current status of fuel that was examined under the CSFM Program is described. Assessments of the effect of boric acid in spent fuel pool water on the corrosion and stress corrosion cracking of stainless steel and the stress corrosion cracking of stainless steel piping containing stagnant water at spent fuel pools are discussed. A list of pertinent publications is included. 84 references, 21 figures, 11 tables

  2. Storage: Asset or albatross?

    Energy Technology Data Exchange (ETDEWEB)

    Peldner, P.J. [Tejas Power Corp., Houston, TX (United States)

    1994-12-31

    Development of storage was originally intended to: act as a surrogate for transportation capacity and capture seasonal commodity differentials. Historically, storage was developed by the pipeline companies as a means to minimize capital expenditures in terms of long haul transportation. By building storage, gas could be transported to the market are and stored using available transportation capacity when load requirements were down in the summer, then withdrawn during the winter season without requiring additional transportation capacity to be built. With the advent of deregulation, gas prices were no longer fixed with respect to time. Due to the seasonal demand of gas, there developed a fairly predictable and very consistent corresponding seasonality to the pricing of natural gas. With this phenomenon, seasonal storage developed an added local distribution companies.

  3. Underground pumped hydroelectric storage

    Science.gov (United States)

    Allen, R. D.; Doherty, T. J.; Kannberg, L. D.

    1984-07-01

    Underground pumped hydroelectric energy storage was conceived as a modification of surface pumped storage to eliminate dependence upon fortuitous topography, provide higher hydraulic heads, and reduce environmental concerns. A UPHS plant offers substantial savings in investment cost over coal-fired cycling plants and savings in system production costs over gas turbines. Potential location near load centers lowers transmission costs and line losses. Environmental impact is less than that for a coal-fired cycling plant. The inherent benefits include those of all pumped storage (i.e., rapid load response, emergency capacity, improvement in efficiency as pumps improve, and capacity for voltage regulation). A UPHS plant would be powered by either a coal-fired or nuclear baseload plant. The economic capacity of a UPHS plant would be in the range of 1000 to 3000 MW. This storage level is compatible with the load-velocity requirements of a greater metropolitan area with population of 1 million or more.

  4. Hydrogen storage compositions

    Science.gov (United States)

    Li, Wen; Vajo, John J.; Cumberland, Robert W.; Liu, Ping

    2011-04-19

    Compositions for hydrogen storage and methods of making such compositions employ an alloy that exhibits reversible formation/deformation of BH.sub.4.sup.- anions. The composition includes a ternary alloy including magnesium, boron and a metal and a metal hydride. The ternary alloy and the metal hydride are present in an amount sufficient to render the composition capable of hydrogen storage. The molar ratio of the metal to magnesium and boron in the alloy is such that the alloy exhibits reversible formation/deformation of BH.sub.4.sup.- anions. The hydrogen storage composition is prepared by combining magnesium, boron and a metal to prepare a ternary alloy and combining the ternary alloy with a metal hydride to form the hydrogen storage composition.

  5. Nuclear Materials Storage

    International Science & Technology Center (ISTC)

    Creation of System for Storage, Operative Control and Physical Protection of Nuclear Materials and Ampoule Sources of Ionizing Radiation (ASIR) on "Baikal-1" stand Complex Meeting the International Requirements of Radioactive Materials Control...

  6. Cloud Storage Vault

    OpenAIRE

    Haver, Eirik; Melvold, Eivind; Ruud, Pål

    2011-01-01

    Today, major IT-companies, such as Microsoft, Amazon and Google, are offering online storage services to their customers. This is a favourable solution -- as opposed to regular storage -- in terms of low costs, reliability, scalability and capacity. However, important security features such as data privacy and integrity are often absent.To address these issues, a cryptographic architecture is proposed that ensures the confidentiality and integrity of the data stored by users, independent of t...

  7. Flywheel Energy Storage Systems

    OpenAIRE

    Daoud, Mohammed; Abdel-Khalik, Ayman; Elserogi, Ahmed; Ahmed, Shehab; Massoud, Ahmed

    2015-01-01

    Flywheels are one of the oldest and most popular energy storage media owing to the simplicity of storing kinetic energy in a rotating mass. Flywheel energy storage systems (FESSs) can be used in different applications, for example, electric utilities and transportation. With the development of new technologies in the field of composite materials and magnetic bearings, higher energy densities are allowed in the design of flywheels. The amount of stored energy in FESS depends on the mass and th...

  8. Spent fuel storage experience

    International Nuclear Information System (INIS)

    Irradiated nuclear fuel has been stored in water pools at essentially all nuclear reactors, beginning with the earliest plants in 1943. Fuel from water-cooled power reactors is clad either with Zircaloy or with stainless steel. Zircaloy-clad fuel has been stored US pools since 1959. Some experimental stainless-steel-clad fuel was stored for 12 yr in the US before reprocessing. Canadian Zircaloy-clad fuel has been stored since 1962. There has been no evidence that the fuel has degraded during pool storage, based principally on visual observations and radiation monitoring of pool air and water. However, several fuel rods have been subjected to metallographic examination after pool exposures up to 11 yr, also with no evidence that the fuel cladding has degraded in the pool. Canadian fuel stored up to 10 yr was returned to a reactor and performed well. Favorable storage experience also has been indicated for other countries with fuel residence times of 5 to 10 yr. The pool storage environment is high-purity water at 5.3 to 7.5 pH, except for pools for pressurized water reactors, which utilize boric acid pool chemistry at 4.5 to 6.0 pH. Pool water temperatures generally range between 20 and 500C. The favorable storage experience, demonstrated technology, successful handling of fuel with reactor-induced defects, benign storage environments, and corrosion-resistant materials offer sufficient bases to proceed with expanded storage capacities and extended fuel storage until questions regarding fuel reprocessing and final storage of nuclear wastes have been resolved. Some surveillance is justified to detect degradation if it becomes significat. Surveillance programs are already under way in several countries. 1 figure, 6 tables

  9. Cryptographic Cloud Storage

    Science.gov (United States)

    Kamara, Seny; Lauter, Kristin

    We consider the problem of building a secure cloud storage service on top of a public cloud infrastructure where the service provider is not completely trusted by the customer. We describe, at a high level, several architectures that combine recent and non-standard cryptographic primitives in order to achieve our goal. We survey the benefits such an architecture would provide to both customers and service providers and give an overview of recent advances in cryptography motivated specifically by cloud storage.

  10. Diffusion local time storage

    OpenAIRE

    Kozlova, M.; Salminen, P.

    2004-01-01

    In this paper we study a storage process or a liquid queue in which the input process is the local time of a positively recurrent stationary diffusion in stationary state and the potential output takes place with a constant deterministic rate. For this storage process we find its stationary distribution and compute the joint distribution of the starting and ending times of the busy and idle periods. This work completes and extends to a more general setting the results in Mannersalo, Norros, a...

  11. Superconducting magnetic energy storage

    International Nuclear Information System (INIS)

    Superconducting inductors provide a compact and efficient means of storing electrical energy without an intermediate conversion process. Energy storage inductors are under development for diurnal load leveling and transmission line stabilization in electric utility systems and for driving magnetic confinement and plasma heating coils in fusion energy systems. Fluctuating electric power demands force the electric utility industry to have more installed generating capacity than the average load requires. Energy storage can increase the utilization of base-load fossil and nuclear power plants for electric utilities. Superconducting magnetic energy storage (SMES) systems, which will store and deliver electrical energy for load leveling, peak shaving, and the stabilization of electric utility networks are being developed. In the fusion area, inductive energy transfer and storage is also being developed by LASL. Both 1-ms fast-discharge theta-pinch and 1-to-2-s slow tokamak energy transfer systems have been demonstrated. The major components and the method of operation of an SMES unit are described, and potential applications of different size SMES systems in electric power grids are presented. Results are given for a 1-GWh reference design load-leveling unit, for a 30-MJ coil proposed stabilization unit, and for tests with a small-scale, 100-kJ magnetic energy storage system. The results of the fusion energy storage and transfer tests are also presented. The common technology base for the systems is discussed

  12. Energy Storage: A Review

    Directory of Open Access Journals (Sweden)

    Himanshu Verma,

    2013-06-01

    Full Text Available Efficient and economic energy storage, if implemented in the current power infrastructure on a large scale, could bring about some of the greatest changes in the power industry in decades. Additionally, energy storage would improve the reliability and dynamic stability of the power system by providing stable, abundant energy reserves that require little ramp time and are less susceptible to varying fuel prices or shortages. Energy storage can shift the higher peak load to off-peak hours in order to level the generation requirement, allowing generators to run more efficiently at a stable power level, potentially decreasing the average cost of electricity. Additionally, increased energy storage capacity can avoid generation capacity, decrease transmission congestion, and help enable distributed generation such as residential solar and wind systems.In this paper energy storage methods are discussed in such a way to provide a detailed overview of how each of the energy storage devices work so that the reader is able to get a better feel for the potential benefits and drawbacks of each device.

  13. Spent nuclear fuel storage

    International Nuclear Information System (INIS)

    When a country becomes self-sufficient in part of the nuclear cycle, as production of fuel that will be used in nuclear power plants for energy generation, it is necessary to pay attention for the best method of storing the spent fuel. Temporary storage of spent nuclear fuel is a necessary practice and is applied nowadays all over the world, so much in countries that have not been defined their plan for a definitive repository, as well for those that already put in practice such storage form. There are two main aspects that involve the spent fuels: one regarding the spent nuclear fuel storage intended to reprocessing and the other in which the spent fuel will be sent for final deposition when the definitive place is defined, correctly located, appropriately characterized as to several technical aspects, and licentiate. This last aspect can involve decades of studies because of the technical and normative definitions at a given country. In Brazil, the interest is linked with the storage of spent fuels that will not be reprocessed. This work analyses possible types of storage, the international panorama and a proposal for future construction of a spent nuclear fuel temporary storage place in the country. (author)

  14. Optical Disk Storage Roadmap

    Science.gov (United States)

    Schechtman, B. H.

    Optical disk technology is the only optical data storage technology with a notable market. After more than a decade of persistent struggle, optical disk storage has established itself as a significant, albeit not dominant, segment of the computer data storage industry. Worldwide revenue for optical disk drives in 1998 was 6.1 billion, which compares with 34.2 billion for magnetic hard disk drives [1]. The success and growth of optical storage in the last few years has been strongly fueled by the emergence and acceptance of the CD-ROM device and media as the de facto standard for software distribution on mass market desktop computer systems. CD-ROM drives accounted for 92% of the optical drive units shipped in 1998, although this represented only 75% of the overall optical drive revenue [1]. For software distribution, the attractive features of CD-ROM are that it is a removable storage medium with very low cost and very high capacity, which can be easily mass-replicated. The ubiquitous presence of CD-ROM drives in desktop computer systems has in turn provided a foundation for the recent growth of recordable and rewritable devices in the CD form factor. This strong, established base will have an important influence on the future development of optical disk storage.

  15. Secure Storage Architectures

    Energy Technology Data Exchange (ETDEWEB)

    Aderholdt, Ferrol [Tennessee Technological University; Caldwell, Blake A [ORNL; Hicks, Susan Elaine [ORNL; Koch, Scott M [ORNL; Naughton, III, Thomas J [ORNL; Pogge, James R [Tennessee Technological University; Scott, Stephen L [Tennessee Technological University; Shipman, Galen M [ORNL; Sorrillo, Lawrence [ORNL

    2015-01-01

    The purpose of this report is to clarify the challenges associated with storage for secure enclaves. The major focus areas for the report are: - review of relevant parallel filesystem technologies to identify assets and gaps; - review of filesystem isolation/protection mechanisms, to include native filesystem capabilities and auxiliary/layered techniques; - definition of storage architectures that can be used for customizable compute enclaves (i.e., clarification of use-cases that must be supported for shared storage scenarios); - investigate vendor products related to secure storage. This study provides technical details on the storage and filesystem used for HPC with particular attention on elements that contribute to creating secure storage. We outline the pieces for a a shared storage architecture that balances protection and performance by leveraging the isolation capabilities available in filesystems and virtualization technologies to maintain the integrity of the data. Key Points: There are a few existing and in-progress protection features in Lustre related to secure storage, which are discussed in (Chapter 3.1). These include authentication capabilities like GSSAPI/Kerberos and the in-progress work for GSSAPI/Host-keys. The GPFS filesystem provides native support for encryption, which is not directly available in Lustre. Additionally, GPFS includes authentication/authorization mechanisms for inter-cluster sharing of filesystems (Chapter 3.2). The limitations of key importance for secure storage/filesystems are: (i) restricting sub-tree mounts for parallel filesystem (which is not directly supported in Lustre or GPFS), and (ii) segregation of hosts on the storage network and practical complications with dynamic additions to the storage network, e.g., LNET. A challenge for VM based use cases will be to provide efficient IO forwarding of the parallel filessytem from the host to the guest (VM). There are promising options like para-virtualized filesystems to help with this issue, which are a particular instances of the more general challenge of efficient host/guest IO that is the focus of interfaces like virtio. A collection of bridging technologies have been identified in Chapter 4, which can be helpful to overcome the limitations and challenges of supporting efficient storage for secure enclaves. The synthesis of native filesystem security mechanisms and bridging technologies led to an isolation-centric storage architecture that is proposed in Chapter 5, which leverages isolation mechanisms from different layers to facilitate secure storage for an enclave. Recommendations: The following highlights recommendations from the investigations done thus far. - The Lustre filesystem offers excellent performance but does not support some security related features, e.g., encryption, that are included in GPFS. If encryption is of paramount importance, then GPFS may be a more suitable choice. - There are several possible Lustre related enhancements that may provide functionality of use for secure-enclaves. However, since these features are not currently integrated, the use of Lustre as a secure storage system may require more direct involvement (support). (*The network that connects the storage subsystem and users, e.g., Lustre s LNET.) - The use of OpenStack with GPFS will be more streamlined than with Lustre, as there are available drivers for GPFS. - The Manilla project offers Filesystem as a Service for OpenStack and is worth further investigation. Manilla has some support for GPFS. - The proposed Lustre enhancement of Dynamic-LNET should be further investigated to provide more dynamic changes to the storage network which could be used to isolate hosts and their tenants. - The Linux namespaces offer a good solution for creating efficient restrictions to shared HPC filesystems. However, we still need to conduct a thorough round of storage/filesystem benchmarks. - Vendor products should be more closely reviewed, possibly to include evaluation of performance/protection of select products. (Note, we are investigation the option of evaluating equipment from Seagate/Xyratex.) Outline: The remainder of this report is structured as follows: - Section 1: Describes the growing importance of secure storage architectures and highlights some challenges for HPC. - Section 2: Provides background information on HPC storage architectures, relevant supporting technologies for secure storage and details on OpenStack components related to storage. Note, that background material on HPC storage architectures in this chapter can be skipped if the reader is already familiar with Lustre and GPFS. - Section 3: A review of protection mechanisms in two HPC filesystems; details about available isolation, authentication/authorization and performance capabilities are discussed. - Section 4: Describe technologies that can be used to bridge gaps in HPC storage and filesystems to facilitate...

  16. Association between sex hormone-binding globulin (SHBG) and metabolic syndrome among men / Associação entre globulina de ligação a hormônio sexual (SHBG) e síndrome metabólica em homens

    Scientific Electronic Library Online (English)

    Emmanuela Quental Callou de, Sá; Francisco Carleial Feijó de, Sá; Kelly Cristina, Oliveira; Fausto, Feres; Ieda Therezinha Nascimento, Verreschi.

    Full Text Available CONTEXTO E OBJETIVO: A síndrome metabólica (SM) consiste em um conjunto de fatores que implicam risco elevado para doenças cardiovasculares. O objetivo foi avaliar a associação entre a globulina ligadora de esteroides sexuais (SHBG), hormônios sexuais e a SM em homens. TIPO DE ESTUDO E LOCAL: [...] Análise retrospectiva de dados do estudo "Estradiol mas não testosterona se correlaciona com doença arterial coronariana em homens", conduzido em um hospital em São Paulo. MÉTODOS: Foram selecionados pacientes do sexo masculino com idade entre 40 e 70 anos, submetidos a angiografia coronária. A idade, a prevalência de dislipidemia, hipertensão e diabetes, o peso, a altura, cintura e o índice de massa corpórea de cada paciente foram coletados. A definição de SM seguiu os critérios do NCEP-ATPIII. Amostras séricas foram coletadas para análises da glicose, colesterol total, colesterol-HDL (high density lipoprotein), triglicerídeos, albumina, SHBG, estradiol e testosterona total (TT). O colesterol-LDL (low density lipoprotein) foi calculado pela fórmula de Friedewald e as testosteronas livre (TL) e biodisponível (TB) pela fórmula de Vermeulen. RESULTADOS: Entraram no estudo 141 pacientes. A prevalência de SM foi significativamente maior no primeiro tercil de SHBG em comparação ao segundo e terceiro tercis. Foi verificada uma associação positiva e significativa ente os valores de SHBG e TT, porém essa associação não foi verificada entre SHBG e TB e TL. CONCLUSÃO: Baixos níveis séricos de SHBG estiveram associados com alta prevalência da SM em pacientes do sexo masculino. Faz-se necessário que estudos avaliem essa associação. Abstract in english CONTEXT AND OBJECTIVE: Metabolic syndrome consists of a set of factors that imply increased risk of cardiovascular diseases. The objective here was to evaluate the association between sex hormone-binding globulin (SHBG), sex hormones and metabolic syndrome among men. DESIGN AND SETTING: Retros [...] pective analysis on data from the study "Endogenous oestradiol but not testosterone is related to coronary artery disease in men", conducted in a hospital in São Paulo. METHODS: Men (aged 40-70) who underwent coronary angiography were selected. The age, weight, height, waist circumference, body mass index and prevalence of dyslipidemia, hypertension and diabetes of each patient were registered. Metabolic syndrome was defined in accordance with the criteria of the Third Report of the National Cholesterol Education Program Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults (NCEP-ATPIII). Serum samples were collected to assess the levels of glucose, total cholesterol, HDL-cholesterol (high density lipoprotein), triglycerides, albumin, SHBG, estradiol and total testosterone (TT). The levels of LDL-cholesterol (low density lipoprotein) were calculated using Friedewald's formula and free testosterone (FT) and bioavailable testosterone (BT) using Vermeulen's formula. RESULTS: 141 patients were enrolled in the study. The prevalence of metabolic syndrome was significantly higher in the first SHBG tercile than in the second and third terciles. A statistically significant positive association between the SHBG and TT values was observed, but no such association was seen between SHBG, BT and FT. CONCLUSION: Low serum levels of SHBG are associated with higher prevalence of metabolic syndrome among male patients, but further studies are required to confirm this association.

  17. Spacecraft cryogenic gas storage systems

    Science.gov (United States)

    Rysavy, G.

    1971-01-01

    Cryogenic gas storage systems were developed for the liquid storage of oxygen, hydrogen, nitrogen, and helium. Cryogenic storage is attractive because of the high liquid density and low storage pressure of cryogens. This situation results in smaller container sizes, reduced container-strength levels, and lower tankage weights. The Gemini and Apollo spacecraft used cryogenic gas storage systems as standard spacecraft equipment. In addition to the Gemini and Apollo cryogenic gas storage systems, other systems were developed and tested in the course of advancing the state of the art. All of the cryogenic storage systems used, developed, and tested to date for manned-spacecraft applications are described.

  18. Short term thermal energy storage

    OpenAIRE

    Abhat, A.

    1980-01-01

    The present paper reviews the problem of short term thermal energy storage for low temperature solar heating applications. The techniques of sensible and latent heat storage are discussed, with particular emphasis on the latter. Requirements for hot water storage subsystems are provided and the importance of stratification in hot water storage tanks is described. Concerning latent heat storage, both material and heat exchanger aspects are considered in detail. The example of a passively opera...

  19. Dry storage of spent fuel

    International Nuclear Information System (INIS)

    Storage of irradiated CANDU fuel in water filled bays is a standard, proven procedure. It has been suggested, however, that dry storage methods might show some advantages in the long term. In particular, a dry storage facility is likely to require less maintenance and produce less secondary waste than a wet storage facility of similar capacity. WNRE has undertaken the development of one dry storage concept - the concrete canister - to assess its feasibility and evaluate its cost. (author)

  20. GAS STORAGE TECHNOLOGY CONSORTIUM

    Energy Technology Data Exchange (ETDEWEB)

    Robert W. Watson

    2004-10-18

    Gas storage is a critical element in the natural gas industry. Producers, transmission and distribution companies, marketers, and end users all benefit directly from the load balancing function of storage. The unbundling process has fundamentally changed the way storage is used and valued. As an unbundled service, the value of storage is being recovered at rates that reflect its value. Moreover, the marketplace has differentiated between various types of storage services, and has increasingly rewarded flexibility, safety, and reliability. The size of the natural gas market has increased and is projected to continue to increase towards 30 trillion cubic feet (TCF) over the next 10 to 15 years. Much of this increase is projected to come from electric generation, particularly peaking units. Gas storage, particularly the flexible services that are most suited to electric loads, is critical in meeting the needs of these new markets. In order to address the gas storage needs of the natural gas industry, an industry-driven consortium was created--the Gas Storage Technology Consortium (GSTC). The objective of the GSTC is to provide a means to accomplish industry-driven research and development designed to enhance operational flexibility and deliverability of the Nation's gas storage system, and provide a cost effective, safe, and reliable supply of natural gas to meet domestic demand. To accomplish this objective, the project is divided into three phases that are managed and directed by the GSTC Coordinator. The first phase, Phase 1A, was initiated on September 30, 2003, and was completed on March 31, 2004. Phase 1A of the project included the creation of the GSTC structure, development and refinement of a technical approach (work plan) for deliverability enhancement and reservoir management. This report deals with Phase 1B and encompasses the period July 1, 2004, through September 30, 2004. During this time period there were three main activities. First was the ongoing negotiations of the four sub-awards working toward signed contracts with the various organizations involved. Second, an Executive Council meeting was held at Penn State September 9, 2004. And third, the GSTC participated in the SPE Eastern Regional Meeting in Charleston, West Virginia, on September 16th and 17th. We hosted a display booth with the Stripper Well Consortium.

  1. GAS STORAGE TECHNOLOGY CONSORTIUM

    Energy Technology Data Exchange (ETDEWEB)

    Robert W. Watson

    2004-07-15

    Gas storage is a critical element in the natural gas industry. Producers, transmission and distribution companies, marketers, and end users all benefit directly from the load balancing function of storage. The unbundling process has fundamentally changed the way storage is used and valued. As an unbundled service, the value of storage is being recovered at rates that reflect its value. Moreover, the marketplace has differentiated between various types of storage services, and has increasingly rewarded flexibility, safety, and reliability. The size of the natural gas market has increased and is projected to continue to increase towards 30 trillion cubic feet (TCF) over the next 10 to 15 years. Much of this increase is projected to come from electric generation, particularly peaking units. Gas storage, particularly the flexible services that are most suited to electric loads, is critical in meeting the needs of these new markets. In order to address the gas storage needs of the natural gas industry, an industry-driven consortium was created--the Gas Storage Technology Consortium (GSTC). The objective of the GSTC is to provide a means to accomplish industry-driven research and development designed to enhance operational flexibility and deliverability of the Nation's gas storage system, and provide a cost effective, safe, and reliable supply of natural gas to meet domestic demand. To accomplish this objective, the project is divided into three phases that are managed and directed by the GSTC Coordinator. Base funding for the consortium is provided by the U.S. Department of Energy (DOE). In addition, funding is anticipated from the Gas Technology Institute (GTI). The first phase, Phase 1A, was initiated on September 30, 2003, and was completed on March 31, 2004. Phase 1A of the project included the creation of the GSTC structure, development and refinement of a technical approach (work plan) for deliverability enhancement and reservoir management. This report deals with Phase 1B and encompasses the period April 1, 2004, through June 30, 2004. During this 3-month period, a Request for Proposals (RFP) was made. A total of 17 proposals were submitted to the GSTC. A proposal selection meeting was held June 9-10, 2004 in Morgantown, West Virginia. Of the 17 proposals, 6 were selected for funding.

  2. Nuclear fuel storage facility

    International Nuclear Information System (INIS)

    Purpose: To increase the spent fuel storage capacity and reduce the installation cost in a nuclear fuel storage facility. Constitution: Fuels handled in the nuclear fuel storage device of the present invention include the following four types: (1) fresh fuels, (2) 100 % reactor core charged fuels, (3) spent fuels just after taking out and (4) fuels after a certain period (for example one half-year) from taking out of the reactor. Reactivity is high for the fuels (1), and some of fuels (2), while low in the fuels (3) (4), Source intensity is strong for the fuels (3) and some of the fuels (2), while it is low for the fuels (1) and (4). Taking notice of the fact that the reactivity, radioactive source intensity and generated after heat are different in the respective fuels, the size of the pool and the storage capacity are increased by the divided storage control. While on the other hand, since the division is made in one identical pool, the control method becomes important, and the working range is restricted by means of a template, interlock, etc., the operation mode of the handling machine is divided into four, etc. for preventing errors. (Kamimura, M.)

  3. WWER spent fuel storage

    International Nuclear Information System (INIS)

    Selection criteria for PAKS NPP dry storage system are outlined. They include the following: fuel temperature in storage; sub-criticality assurance (avoidance of criticality for fuel in the unirradiated condition without having to take credit for burn-up); assurance of decay heat removal; dose uptake to the operators and public; protection of environment; volume of waste produced during operation and decommissioning; physical protection of stored irradiated fuel assemblies; IAEA safeguards assurance; storage system versus final disposal route; cost of construction and extent of technology transfer to Hungarian industry. Several available systems are evaluated against these criteria, and as a result the GEC ALSTHOM Modular Vault Dry Store (MVDS) system has been selected. The MVDS is a passively cooled dry storage facility. Its most important technical, safety, licensing and technology transfer characteristics are outlined. On the basis of the experience gained some key questions and considerations related to the East European perspective in the field of spent fuel storage are discussed. 8 figs

  4. Radioactive waste storage issues

    International Nuclear Information System (INIS)

    In the United States we generate greater than 500 million tons of toxic waste per year which pose a threat to human health and the environment. Some of the most toxic of these wastes are those that are radioactively contaminated. This thesis explores the need for permanent disposal facilities to isolate radioactive waste materials that are being stored temporarily, and therefore potentially unsafely, at generating facilities. Because of current controversies involving the interstate transfer of toxic waste, more states are restricting the flow of wastes into - their borders with the resultant outcome of requiring the management (storage and disposal) of wastes generated solely within a state's boundary to remain there. The purpose of this project is to study nuclear waste storage issues and public perceptions of this important matter. Temporary storage at generating facilities is a cause for safety concerns and underscores, the need for the opening of permanent disposal sites. Political controversies and public concern are forcing states to look within their own borders to find solutions to this difficult problem. Permanent disposal or retrievable storage for radioactive waste may become a necessity in the near future in Colorado. Suitable areas that could support - a nuclear storage/disposal site need to be explored to make certain the health, safety and environment of our citizens now, and that of future generations, will be protected

  5. Energy storage connection system

    Science.gov (United States)

    Benedict, Eric L.; Borland, Nicholas P.; Dale, Magdelena; Freeman, Belvin; Kite, Kim A.; Petter, Jeffrey K.; Taylor, Brendan F.

    2012-07-03

    A power system for connecting a variable voltage power source, such as a power controller, with a plurality of energy storage devices, at least two of which have a different initial voltage than the output voltage of the variable voltage power source. The power system includes a controller that increases the output voltage of the variable voltage power source. When such output voltage is substantially equal to the initial voltage of a first one of the energy storage devices, the controller sends a signal that causes a switch to connect the variable voltage power source with the first one of the energy storage devices. The controller then causes the output voltage of the variable voltage power source to continue increasing. When the output voltage is substantially equal to the initial voltage of a second one of the energy storage devices, the controller sends a signal that causes a switch to connect the variable voltage power source with the second one of the energy storage devices.

  6. Technology Roadmap: Energy Storage

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2014-03-01

    Energy storage technologies are valuable components in most energy systems and could be an important tool in achieving a low-carbon future. These technologies allow for the decoupling of energy supply and demand, in essence providing a valuable resource to system operators. There are many cases where energy storage deployment is competitive or near-competitive in today's energy system. However, regulatory and market conditions are frequently ill-equipped to compensate storage for the suite of services that it can provide. Furthermore, some technologies are still too expensive relative to other competing technologies (e.g. flexible generation and new transmission lines in electricity systems). One of the key goals of this new roadmap is to understand and communicate the value of energy storage to energy system stakeholders. This will include concepts that address the current status of deployment and predicted evolution in the context of current and future energy system needs by using a ''systems perspective'' rather than looking at storage technologies in isolation.

  7. Extended storage of spent fuel

    International Nuclear Information System (INIS)

    This document is the final report on the IAEA Co-ordinated Research Programme on the Behaviour of Spent Fuel and Storage Facility Components during Long Term Storage (BEFAST-II, 1986-1991). It contains the results on wet and dry spent fuel storage technologies obtained from 16 organizations representing 13 countries who participated in the co-ordinated research programme. Considerable quantities of spent fuel continue to arise and accumulate. Many countries are investigating the option of extended spent fuel storage prior to reprocessing or fuel disposal. Wet storage continues to predominate as an established technology with the construction of additional away-from-reactor storage pools. However, dry storage is increasingly used with most participants considering dry storage concepts for the longer term. Depending on the cladding type options of dry storage in air or inert gas are proposed. Dry storage is becoming widely used as a supplement to wet storage for zirconium alloy clad oxide fuels. Storage periods as long as under wet conditions appear to be feasible. Dry storage will also continue to be used for Al clad and Magnox type fuel. Enhancement of wet storage capacity will remain an important activity. Rod consolidation to increase wet storage capacity will continue in the UK and is being evaluated for LWR fuel in the USA, and may start in some other countries. High density storage racks have been successfully introduced in many existing pools and are planned for future facilities. For extremely long wet storage (?50 years), there is a need to continue work on fuel integrity investigations and LWR fuel performance modelling. it might be that pool component performance in some cases could be more limiting than the FA storage performance. It is desirable to make concerted efforts in the field of corrosion monitoring and prediction of fuel cladding and poll component behaviour in order to maintain good experience of wet storage. Refs, figs and tabs

  8. Costs of Archival Storage

    DEFF Research Database (Denmark)

    Nielsen, Anders Bo; Thirifays, Alex; Kejser, Ulla Bøgvad

    2012-01-01

    This paper presents an analysis of the cost of archival storage. The study is part of a project conducted by The Danish National Archives, The Royal Library, and The State and University Library to develop a generic cost model for digital preservation (CMDP). The purposes of the study were to...... determine the costs of establishing and maintaining a preservation solution destined for long-term preservation of digital materials and to develop a tool capable of doing this operation. In order to fulfill the purposes, the project employed a combination of own and external experience as well as the OAIS...... Reference Model as a framework to fully understand and identify the cost critical activities of bit-preservation as described in Archival Storage. We found that the costs of Archival Storage are obviously closely linked to the data volume, but also to the required preservation quality, especially with...

  9. Retractable storage lug

    International Nuclear Information System (INIS)

    Inspecting and refueling a nuclear reactor requires the removal and storage of various reactor components until the job is completed. In particular, current practice calls for an indexing fixture to be stored separately from a previously withdrawn plenum assembly. In the present invention the indexing fixture may be stacked directly upon the plenum assembly, thereby resulting in a savings in both floor storage space and downtime. The indexing fixture is equipped with a plurality of rotatable and retractable storage lugs, each equipped with a rotatable and slidable weight-cylinder assembly in coaxial relationship with a pivoted support beam. The weight-cylinder assembly and support beam are connected together by a lockable channel, groove and pin arrangement. Provision is allowed for remote activation of the lug

  10. Plutonium storage phenomenology

    International Nuclear Information System (INIS)

    Plutonium has been produced, handled, and stored at Department of Energy (DOE) facilities since the 1940s. Many changes have occurred during the last 40 years in the sources, production demands, and end uses of plutonium. These have resulted in corresponding changes in the isotopic composition as well as the chemical and physical forms of the processed and stored plutonium. Thousands of ordinary food pack tin cans have been used successfully for many years to handle and store plutonium. Other containers have been used with equal success. This paper addressees the exceptions to this satisfactory experience. To aid in understanding the challenges of handling plutonium for storage or immobilization the lessons learned from past storage experience and the necessary countermeasures to improve storage performance are discussed

  11. Evolution of clustered storage

    CERN Document Server

    CERN. Geneva; Van de Vyvre, Pierre

    2007-01-01

    The session actually featured two presentations: * Evolution of clustered storage by Lance Hukill, Quantum Corporation * ALICE DAQ - Usage of a Cluster-File System: Quantum StorNext by Pierre Vande Vyvre, CERN-PH the second one prepared at short notice by Pierre (thanks!) to present how the Quantum technologies are being used in the ALICE experiment. The abstract to Mr Hukill's follows. Clustered Storage is a technology that is driven by business and mission applications. The evolution of Clustered Storage solutions starts first at the alignment between End-users needs and Industry trends: * Push-and-Pull between managing for today versus planning for tomorrow * Breaking down the real business problems to the core applications * Commoditization of clients, servers, and target devices * Interchangeability, Interoperability, Remote Access, Centralized control * Oh, and yes, there is a budget and the "real world" to deal with This presentation will talk through these needs and trends, and then ask the question, ...

  12. REDOX electrochemical energy storage

    Science.gov (United States)

    Thaller, L. H.

    1980-01-01

    Reservoirs of chemical solutions can store electrical energy with high efficiency. Reactant solutions are stored outside conversion section where charging and discharging reactions take place. Conversion unit consists of stacks of cells connected together in parallel hydraulically, and in series electrically. Stacks resemble fuel cell batteries. System is 99% ampere-hour efficient, 75% watt hour efficient, and has long projected lifetime. Applications include storage buffering for remote solar or wind power systems, and industrial load leveling. Cost estimates are $325/kW of power requirement plus $51/kWh storage capacity. Mass production would reduce cost by about factor of two.

  13. Nuclear fuel storage

    International Nuclear Information System (INIS)

    A method and apparatus for the storage of fuel in a stainless steel egg crate structure within a storage pool are described. Fuel is initially stored in a checkerboard pattern or in each opening if the fuel is of low enrichment. Additional fuel (or fuel of higher enrichment) is later stored by adding stainless steel angled plates within each opening, thereby forming flux traps between the openings. Still higher enrichment fuel is later stored by adding poison plates either with or without the stainless steel angles. 8 claims

  14. ENERGY STORAGE SYSTEMS

    Directory of Open Access Journals (Sweden)

    ELENA RADUCAN

    2011-02-01

    Full Text Available In this paper you will find an overview of systems and energy storage techniques and a comparison of the important characteristics of them. Delocalized electricity production and the introduction of variable, fluctuating sources (renewable energy: solar, wind turbines, etc. increase the difficulty of stabilizing the power network, mainly due to a supply-demand imbalance. It is therefore convenient to generate the energy, transmit it, convert it, and then store it if need be. More than ever then, the storage of electrical energy has become a necessity.

  15. Electrochemical energy storage

    CERN Document Server

    Tarascon, Jean-Marie

    2015-01-01

    The electrochemical storage of energy has become essential in assisting the development of electrical transport and use of renewable energies. French researchers have played a key role in this domain but Asia is currently the market leader. Not wanting to see history repeat itself, France created the research network on electrochemical energy storage (RS2E) in 2011. This book discusses the launch of RS2E, its stakeholders, objectives, and integrated structure that assures a continuum between basic research, technological research and industries. Here, the authors will cover the technological

  16. B-cell-rich T-cell lymphoma associated with Epstein-Barr virus-reactivation and T-cell suppression following antithymocyte globulin therapy in a patient with severe aplastic anemia

    Directory of Open Access Journals (Sweden)

    Nobuyoshi Hanaoka

    2015-09-01

    Full Text Available B-cell lymphoproliferative disorder (B-LPD is generally characterized by the proliferation of Epstein-Barr virus (EBV-infected B lymphocytes. We here report the development of EBV-negative B-LPD associated with EBV-reactivation following antithymocyte globulin (ATG therapy in a patient with aplastic anemia. The molecular autopsy study showed the sparse EBV-infected clonal T cells could be critically involved in the pathogenesis of EBV-negative oligoclonal B-LPD through cytokine amplification and escape from T-cell surveillances attributable to ATG-based immunosuppressive therapy, leading to an extremely rare B-cell-rich T-cell lymphoma. This report helps in elucidating the complex pathophysiology of intractable B-LPD refractory to rituximab.

  17. B-Cell-Rich T-Cell Lymphoma Associated with Epstein-Barr Virus-Reactivation and T-Cell Suppression Following Antithymocyte Globulin Therapy in a Patient with Severe Aplastic Anemia.

    Science.gov (United States)

    Hanaoka, Nobuyoshi; Murata, Shogo; Hosoi, Hiroki; Shimokado, Aiko; Mushino, Toshiki; Kuriyama, Kodai; Hatanaka, Kazuo; Nishikawa, Akinori; Kurimoto, Miwa; Sonoki, Takashi; Muragaki, Yasuteru; Nakakuma, Hideki

    2015-09-23

    B-cell lymphoproliferative disorder (B-LPD) is generally characterized by the proliferation of Epstein-Barr virus (EBV)-infected B lymphocytes. We here report the development of EBV-negative B-LPD associated with EBV-reactivation following antithymocyte globulin (ATG) therapy in a patient with aplastic anemia. The molecular autopsy study showed the sparse EBV-infected clonal T cells could be critically involved in the pathogenesis of EBV-negative oligoclonal B-LPD through cytokine amplification and escape from T-cell surveillances attributable to ATG-based immunosuppressive therapy, leading to an extremely rare B-cell-rich T-cell lymphoma. This report helps in elucidating the complex pathophysiology of intractable B-LPD refractory to rituximab. PMID:26487932

  18. Prevención de la isoinmunización materna al RhD, con gamma-globulina anti-D / Prevention of maternal RhD isoimmunization with anti-D gamma globulin

    Scientific Electronic Library Online (English)

    Héctor Alfredo, Baptista-González; Fany, Rosenfeld-Mann; Teresita, Leiss-Márquez.

    2001-02-01

    Full Text Available OBJETIVO: Presentar la experiencia institucional en la prevención de la isoinmunización al RhD, mediante el empleo de 150 µg de gamma-globulina anti-D en las mujeres Rh negativo. MATERIAL Y MÉTODOS: Se registraron los antecedentes inmunohematológicos de los casos consecutivos de todas las mujeres Rh [...] negativo que acudieron, para su atención médica, al Instituto Nacional de Perinatología entre 1982 y 1995. A las mujeres con riesgo de isoinmunización se les aplicó 150 µg de gamma-globulina anti-D, con fines preventivos. RESULTADOS: En el periodo de estudio ingresaron 4 857 mujeres Rh negativo (4.85% del total de mujeres), de las cuales 629 (13.0%), presentaron isoinmunización al RhD. De estas últimas, 542 (86.2%) ya se encontraron isoinmunizadas desde antes de su ingreso al Instituto. En 22 casos (3.5%), la isoinmunización ocurrió a pesar de que recibieron la dosis adecuada de gamma-globulina anti D. De las 2 605 pacientes (53.6%) sometidas a prevención, a 2 039 se les aplicó una sola dosis, y a 475, hasta dos dosis. En 22 casos se documentó la falla de la prevención; sin embargo, en cuatro de ellos, se registraron embarazos múltiples, y los restantes 18 presentaron patología obstétrica asociada. CONCLUSIONES: Mediante este programa de prevención, consistente en administrar 150 µg de gamma-globulina anti-D por dosis, es posible reducir la isoinmunización a menos de un caso por cada 1 000 mujeres. Los fracasos en la prevención de la isoinmunización se asociaron a condiciones obstétricas agregadas y al incumplimiento de las guías o lineamientos del programa. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.html Abstract in english OBJECTIVE: To report our experience in preventing RhD maternal isoimmunization by using anti-D gamma globulin among Rh-negative women. MATERIAL AND METHODS: Between 1982 and 1995, immunologic and hematologic data were collected from all Rh-negative women seen at Mexico's National Perinatology Instit [...] ute. Women at risk of Rh isoimmunization were given a prophylactic dose of 150 µg of anti-D gamma globulin. RESULTS: A total of 4 857 Rh-negative women were seen during the study period (4.85% of the total population of women seen at the Institute), 629 (13.0%) of whom developed RhD isoimmunization; 542 (86.2%) of these women were already isoimmunized when first seen at our Institute. Twenty-two women (3.5%) developed isoimmunization even after receiving a proper dose of anti-D gamma globulin. Prophylaxis was given to 2 605 women (53.6%); 2 039 received a single dose, and 475 two doses. Prophylaxis failed in 22 cases; four were women with multiple pregnancy and 18 developed obstetric pathologic conditions. CONCLUSIONS: The use of anti-D gamma globulin resulted in a reduction of maternal Rh isoimmunization to less than one case per 1 000 women. Failures to prevent isoimmunization were associated to additional obstetric conditions and to lack of adherence to prevention guidelines. The English version of this paper is available at: http://www.insp.mx/salud/index.html

  19. TRIGA spent fuel storage

    International Nuclear Information System (INIS)

    Storage of spent fuel elements is a step preliminary to final radioactive waste disposal operation. The spent fuel issue will have a common solution for both spent fuel from Cernavoda NPP and research TRIGA reactors currently operated in Romania. For the case of TRIGA reactor spent fuel this will be an alternative solution to the now functioning alternative of 'on site' storing solution adopted so far at INR Pitesti. For the time being the short term storage requirements for TRIGA spent fuel are adequately fulfilled by the pool of a multizonal reactor, the construction of which was definitively stopped. On the other hand the HEU - LEU conversion of the 14 MW TRIGA reactor which will be completed till May 2006, will pose not spent fuel problems as the TRIGA HEU fuel (612 elements) will be transferred in US (not later than May 2009). Consequently, the needs for intermediate storage will be associated only with the LEU spent fuel from TRIGA LEU-SSR and TRIGA LEU-ACPR reactors. In the latter case the maximum number of elements will be 167. For the stationary 14 MW (SSR) reactor but the amount of fuel elements to be stored on a intermediate term will be a function of service span of this reactor as well of the degree of request. Totally, some 1,750 SSR-LEU fuel elements will require intermediate storage. There is a preliminary agreement with 'NUCLEARELECTRICA -S.A.' Company regarding LEU TRIGA spent fuel storage at the intermediate storage facility for spent fuel of Cernavoda NPP.. A safety investigation is underway to determine the impact of LEU spent fuel upon the dry environment containing spent CANDU fuel. To fulfil the requirements imposed by CANDU storage technology the LEU spent fuel will be correspondingly conditioned. Then adequate containers will be used for transportation of fuel to Cernavoda's storage cell. Subcriticality condition in the storage cell loaded with LEU was checked by calculating the multiplication factor for an infinite lattice. The calculation method is based on a model according to which every TRIGA-LEU fuel element from a batch of 15 elements are cut into two equal pieces and encapsulated in stainless steel cans. The 30 encapsulated pieces are then introduced inside a capsule - a cylindrical container of CANDU fuel element cluster sizes. The empty space between fuel elements will be filled with borosilicate glass granules. Computations were carried out for burnup values of 40% and 61.5% of initial 235 U as well for fresh fuel. Dry and wet environment storage conditions were also considered. The results indicate that in the presence of borosilicate glass TRIGA-LEU fuel element fulfils the subcriticality condition irrespective of burnup value. Intermediate storage possibilities using both expertise and technological opportunities available at INR Pitesti are also considered

  20. Sex hormone binding globulin decrease as potential pathogenetic factor for hirsutism in adolescent girls Disminución de la globulina transportadora de hormonas sexuales como factor patogénico de hirsutismo en la adolescencia

    Directory of Open Access Journals (Sweden)

    Graciela Cross

    2008-04-01

    Full Text Available We investigated 252 non-obese female subjects aged 13-39 years to evaluate if an exaggerated descent of sex hormone binding globulin (SHBG levels during adolescence can play a role in the development of hirsutism. Body hair was assessed according to Ferriman and Gallwey (FG, with a stringent criterion of normality of 4 and controls (FG Se investigaron 252 mujeres con peso normal, de 13 a 39 años de edad, para evaluar si un descenso exagerado en los niveles de la globulina transportadora de hormonas sexuales ("sex hormone binding globulin"; SHBG puede tener un rol en el desarrollo de hirsutismo. Este signo fue evaluado con la escala de Ferriman y Gallwey (FG, empleando un criterio riguroso de normalidad 4 y controles (FG < 4, ciclos menstruales regulares, sin acné. En adolescentes de 15-18 años, los valores de SHBG fueron menores en las "hirsutas", los niveles de FT fueron similares en ambos grupos y el índice de FG correlacionó inversamente con SHBG. En las mujeres de 19-39 años, los niveles de FT fueron mayores en las "hirsutas", los valores de SHBG fueron similares en ambos grupos y FG correlacionó positivamente con FT. Los valores más bajos de SHBG se observaron entre 15 y 18 años, pero la pendiente de disminución a partir de los valores de 13-14 años fue mayor en el grupo de "hirsutas". Los valores de FT se incrementaron progresivamente con la edad, pero el aumento fue mayor en el grupo de "hirsutas". Estos resultados sugieren un rol importante del descenso de SHBG en la adolescencia vs. un incremento más acentuado de los niveles de testosterona en las adultas, como factores que condicionan el desarrollo del hirsutismo en esos dos diferentes periodos de la vida.

  1. Antibodies to glycosylphosphatidyl-inositol anchored proteins (GPI-AP) in antithymocyte and antilymphocyte globulin: possible role for the expansion of GPI-AP deficient cells in aplastic anemia.

    Science.gov (United States)

    Breitinger, Heike H; Rojewski, Markus T; Schrezenmeier, Hubert

    2009-09-01

    Antithymocyte globulin (ATG) and antilymphocyte globulin (ALG) are currently used successfully for immunosuppressive treatment of aplastic anemia. In this study we have investigated whether commercial ATG/ALG preparations contain antibodies against glycosylphosphatidyl-inositol anchored proteins (GPI-AP), which could be responsible for emergence of GPI-deficient populations in aplastic anemia after ATG/ALG therapy. We analyzed four commercial ATG/ALG preparations by competitive binding assays using flow cytometry. Quantification was achieved by calculating the concentration of ATG/ALG required to give 50% inhibition of binding the specific fluorochrome-labeled monoclonal antibody (EC50). High concentrations of antibodies against the GPI-anchored protein CD52 were found in all preparations (Lymphoglobulin Genzyme, Thymoglobulin Genzyme, ATGAM. Pharmacia & Upjohn, and ATG-Fresenius S Fresenius). Antibodies against the GPI-anchored protein CD48 are present in significant concentrations except in the preparation ATGAM. CD16 antibodies were found in lower concentrations. We could not detect significant concentrations of antibodies against the GPI-anchored proteins CD157 and CD14. Campath-1H, a monoclonal antibody against the GPI-anchored protein CD52, has been used as immunosuppressive tool for T-cell depletion. CD52 antibodies in ATG/ALG preparations might contribute in the same way to the immunosuppressive effects in treatment of aplastic anemia. It is known that in a substantial proportion of patients with aplastic anemia GPI-deficient cells are present in a low level at diagnosis or emerge after immunosuppressive therapy. GPI-anchored antibodies in ATG/ALG preparations might lead to a relative advantage for pre-existing GPI-deficient cells caused by an escape from the antibody-mediated attack. PMID:19137453

  2. Utility spent fuel storage experience

    International Nuclear Information System (INIS)

    Past experience with storage of spent fuel elements in pools are reviewed. Data are presented which demonstrate that stainless and Zircaloy-clad spent light water reactor fuel elements have been stored for periods of 12 to 18 years, respectively, without damage due to storage in pools. Techniques for storing elements damaged in the reactor or in transfer to storage are also described. Routine surveillance and exploratory fuel examinations during storage are recommended. It is recommended that past experiences would justify expansion of fuel storage facilities and extension of storage time for commercial water reactor fuel

  3. Spent-fuel-storage alternatives

    Energy Technology Data Exchange (ETDEWEB)

    1980-01-01

    The Spent Fuel Storage Alternatives meeting was a technical forum in which 37 experts from 12 states discussed storage alternatives that are available or are under development. The subject matter was divided into the following five areas: techniques for increasing fuel storage density; dry storage of spent fuel; fuel characterization and conditioning; fuel storage operating experience; and storage and transport economics. Nineteen of the 21 papers which were presented at this meeting are included in this Proceedings. These have been abstracted and indexed. (ATT)

  4. Hydrogen storage materials the characterisation of their storage properties

    CERN Document Server

    Broom, Darren P

    2011-01-01

    How to store hydrogen safely is a major technical obstacle to our future transition to a 'hydrogen economy'. As reversible solid-state storage gains currency as a solution, this book characterizes the hydrogen storage properties of the candidate materials.

  5. Abstract Storage Devices

    CERN Document Server

    Koenig, Robert; Tessaro, Stefano

    2007-01-01

    A quantum storage device differs radically from a conventional physical storage device. Its state can be set to any value in a certain (infinite) state space, but in general every possible read operation yields only partial information about the stored state. The purpose of this paper is to initiate the study of a combinatorial abstraction, called abstract storage device (ASD), which models deterministic storage devices with the property that only partial information about the state can be read, but that there is a degree of freedom as to which partial information should be retrieved. This concept leads to a number of interesting problems which we address, like the reduction of one device to another device, the equivalence of devices, direct products of devices, as well as the factorization of a device into primitive devices. We prove that every ASD has an equivalent ASD with minimal number of states and of possible read operations. Also, we prove that the reducibility problem for ASD's is NP-complete, that t...

  6. Inertial storage for satellites

    Energy Technology Data Exchange (ETDEWEB)

    Eisenhaure, D.

    1984-11-01

    A new system is being developed that performs satellite attitude control, attitude reference, and energy storage utilizing inertia wheels. The baseline approach consists of two counter rotating flywheels suspended in specially designed magnetic bearings, spin axis motor/generators, and a control system. The control system regulates the magnetic bearings and spin axis motor/generators and interacts with other satellite subsystems (photovoltaic array, star trackers, Sun sensors, magnetic torquers, etc.) to perform the three functions. Existing satellites utilize separate subsystems to perform attitude control, provide attitude reference, and store energy. These functions are currently performed using reaction or momentum wheels, gyros, batteries, and devices that provide an absolute reference (Sun sensors and star trackers). A Combined Attitude, Reference, and Energy Storage (CARES) system based on high energy density inertial energy storage wheels (flywheels) has potential advantages over existing technologies. Even when used only for energy storage, this system offers the potential for substantial improvements in life, energy efficiency, and weight over existing battery technologies. Utilizing this same device for both attitude control and attitude reference would result in significant additional savings in overall satellite weight and complexity.

  7. Muon storage ring

    CERN Multimedia

    1974-01-01

    The muon storage ring where the g-2 of the muon is being measured with extremely high accuracy. The ring is 14-m in diameter and has very precise magnetic bending and electric focussing fields so that the muons orbit the ring under well-defined conditions.

  8. NGLW RCRA Storage Study

    International Nuclear Information System (INIS)

    The Idaho Nuclear Technology and Engineering Center (INTEC) at the Idaho National Engineering and Environmental Laboratory contains radioactive liquid waste in underground storage tanks at the INTEC Tank Farm Facility (TFF). INTEC is currently treating the waste by evaporation to reduce the liquid volume for continued storage, and by calcination to reduce and convert the liquid to a dry waste form for long-term storage in calcine bins. Both treatment methods and activities in support of those treatment operations result in Newly Generated Liquid Waste (NGLW) being sent to TFF. The storage tanks in the TFF are underground, contained in concrete vaults with instrumentation, piping, transfer jets, and managed sumps in case of any liquid accumulation in the vault. The configuration of these tanks is such that Resource Conservation and Recovery Act (RCRA) regulations apply. The TFF tanks were assessed several years ago with respect to the RCRA regulations and they were found to be deficient. This study considers the configuration of the current tanks and the RCRA deficiencies identified for each. The study identifies four potential methods and proposes a means of correcting the deficiencies. The cost estimates included in the study account for construction cost; construction methods to minimize work exposure to chemical hazards, radioactive contamination, and ionizing radiation hazards; project logistics; and project schedule. The study also estimates the tank volumes benefit associated with each corrective action to support TFF liquid waste management planning

  9. Interim onsite radwaste storage

    International Nuclear Information System (INIS)

    The problems associated with state compacts for disposing of radioactive waste are well known. Presently, it appears that no compact will be capable of receiving waste by January 1996. Most compacts and independent states are essentially at ground zero. Politics, the open-quotes Not in MY backyardclose quotes (NIMBY) factor, poor public relations, public mistrust, public unawareness of engineering radwaste disposal, unfavorable media hype, and a multitude of other issues will delay projects even further. The financial burden imposed on electric utility rate payers is monumental. An economical and viable solution is onsite radwaste storage at nuclear stations. For example, the Bechtel design can store waste for $32/ft3, whereas burial costs per cubic foot are approximately $300.00/ft3 for a LSA box, $350.00/ft3 for low-level resins, and $570.00/ft3 for high-level resins, plus transportation costs of approximately $2.00 per mile. This Bechtel onsite radwaste storage is designed for maximum radwaste storage per square foot. In the overall design, emphasis was placed upon operations, maintenance, health physics, personnel, radiation exposure, and economics. Many utility personnel were consulted for their input. The final design has encompassed most of these views to provide the optimum onsite storage facility

  10. Tuber storage proteins.

    Science.gov (United States)

    Shewry, Peter R

    2003-06-01

    A wide range of plants are grown for their edible tubers, but five species together account for almost 90 % of the total world production. These are potato (Solanum tuberosum), cassava (Manihot esculenta), sweet potato (Ipomoea batatus), yams (Dioscorea spp.) and taro (Colocasia, Cyrtosperma and Xanthosoma spp.). All of these, except cassava, contain groups of storage proteins, but these differ in the biological properties and evolutionary relationships. Thus, patatin from potato exhibits activity as an acylhydrolase and esterase, sporamin from sweet potato is an inhibitor of trypsin, and dioscorin from yam is a carbonic anhydrase. Both sporamin and dioscorin also exhibit antioxidant and radical scavenging activity. Taro differs from the other three crops in that it contains two major types of storage protein: a trypsin inhibitor related to sporamin and a mannose-binding lectin. These characteristics indicate that tuber storage proteins have evolved independently in different species, which contrasts with the highly conserved families of storage proteins present in seeds. Furthermore, all exhibit biological activities which could contribute to resistance to pests, pathogens or abiotic stresses, indicating that they may have dual roles in the tubers. PMID:12730067

  11. Fuel storage rack

    International Nuclear Information System (INIS)

    Disclosed is a storage rack for spent nuclear fuel elements comprising a multiplicity of elongated hollow containers of uniform cross-section, preferably square,some of said containers having laterally extending continuous flanges extending between adjacent containers and defining continuous elongated chambers therebetween for the reception of neutron absorbing panels. 18 claims, 7 figures

  12. Solar Energy: Heat Storage.

    Science.gov (United States)

    Knapp, Henry H., III

    This module on heat storage is one of six in a series intended for use as supplements to currently available materials on solar energy and energy conservation. Together with the recommended texts and references (sources are identified), these modules provide an effective introduction to energy conservation and solar energy technologies. The module…

  13. Storage ring beam tests

    International Nuclear Information System (INIS)

    Storage ring beam tests since the Second Workshop on RF-Superconductivity (July 1984) are discussed in this report. The experiments are listed in chronological order. Details depend on information given in papers and/or by private communication. 14 references, 22 figures, 5 tables

  14. Pneumatic energy storage

    Energy Technology Data Exchange (ETDEWEB)

    Flowers, D.

    1995-09-19

    An essential component to hybrid electric and electric vehicles is energy storage. A power assist device could also be important to many vehicle applications. This discussion focuses on the use of compressed gas as a system for energy storage and power in vehicle systems. Three possible vehicular applications for which these system could be used are discussed in this paper. These applications are pneumatically driven vehicles, series hybrid electric vehicles, and power boost for electric and conventional vehicles. One option for a compressed gas system is as a long duration power output device for purely pneumatic and hybrid cars. This system must provide enough power and energy to drive under normal conditions for a specified time or distance. The energy storage system for this use has the requirement that it will be highly efficient, compact, and have low mass. Use of a compressed gas energy storage as a short duration, high power output system for conventional motor vehicles could reduce engine size or reduce transient emissions. For electric vehicles this kind of system could lengthen battery life by providing battery load leveling during accelerations. The system requirements for this application are that it be compact and have low mass. The efficiency of the system is a secondary consideration in this application.

  15. NGLW RCRA Storage Study

    Energy Technology Data Exchange (ETDEWEB)

    R. J. Waters; R. Ochoa; K. D. Fritz; D. W. Craig

    2000-06-01

    The Idaho Nuclear Technology and Engineering Center (INTEC) at the Idaho National Engineering and Environmental Laboratory contains radioactive liquid waste in underground storage tanks at the INTEC Tank Farm Facility (TFF). INTEC is currently treating the waste by evaporation to reduce the liquid volume for continued storage, and by calcination to reduce and convert the liquid to a dry waste form for long-term storage in calcine bins. Both treatment methods and activities in support of those treatment operations result in Newly Generated Liquid Waste (NGLW) being sent to TFF. The storage tanks in the TFF are underground, contained in concrete vaults with instrumentation, piping, transfer jets, and managed sumps in case of any liquid accumulation in the vault. The configuration of these tanks is such that Resource Conservation and Recovery Act (RCRA) regulations apply. The TFF tanks were assessed several years ago with respect to the RCRA regulations and they were found to be deficient. This study considers the configuration of the current tanks and the RCRA deficiencies identified for each. The study identifies four potential methods and proposes a means of correcting the deficiencies. The cost estimates included in the study account for construction cost; construction methods to minimize work exposure to chemical hazards, radioactive contamination, and ionizing radiation hazards; project logistics; and project schedule. The study also estimates the tank volumes benefit associated with each corrective action to support TFF liquid waste management planning.

  16. NV Energy Electricity Storage Valuation

    Energy Technology Data Exchange (ETDEWEB)

    Ellison, James F.; Bhatnagar, Dhruv; Samaan, Nader A.; Jin, Chunlian

    2013-06-30

    This study examines how grid-level electricity storage may benet the operations of NV Energy in 2020, and assesses whether those benets justify the cost of the storage system. In order to determine how grid-level storage might impact NV Energy, an hourly production cost model of the Nevada Balancing Authority (\\BA") as projected for 2020 was built and used for the study. Storage facilities were found to add value primarily by providing reserve. Value provided by the provision of time-of-day shifting was found to be limited. If regulating reserve from storage is valued the same as that from slower ramp rate resources, then it appears that a reciprocating engine generator could provide additional capacity at a lower cost than a pumped storage hydro plant or large storage capacity battery system. In addition, a 25-MW battery storage facility would need to cost $650/kW or less in order to produce a positive Net Present Value (NPV). However, if regulating reserve provided by storage is considered to be more useful to the grid than that from slower ramp rate resources, then a grid-level storage facility may have a positive NPV even at today's storage system capital costs. The value of having storage provide services beyond reserve and time-of-day shifting was not assessed in this study, and was therefore not included in storage cost-benefit calculations.

  17. Entangled Cloud Storage

    DEFF Research Database (Denmark)

    Ateniese, Giuseppe; Dagdelen, Özgür

    2012-01-01

    Entangled cloud storage enables a set of clients {P_i} to “entangle” their files {f_i} into a single clew c to be stored by a (potentially malicious) cloud provider S. The entanglement makes it impossible to modify or delete significant part of the clew without affecting all files in c. A clew keeps the files in it private but still lets each client P_i recover his own data by interacting with S; no cooperation from other clients is needed. At the same time, the cloud provider is discouraged from altering or overwriting any significant part of c as this will imply that none of the clients can recover their files. We provide theoretical foundations for entangled cloud storage, introducing the notion of an entangled encoding scheme that guarantees strong security requirements capturing the properties above. We also give a concrete construction based on privacy-preserving polynomial interpolation, along with protocols for using the encoding scheme in practice. Protocols for cloud storage find applicationin the cloud setting, where clients store their files on a remote server and need to be ensured that the cloud provider will not delete their data illegitimately. Current solutions, e.g., based on Provable Data Possession and Proof of Retrievability, catch a malicious server “after-the-fact”, meaning that the server needs to be challenged regularly to provide evidence that the clients’ files are stored at a given time. Entangled storage makes all clients equal and with the same rights: It makes it financially inconvenient for a cloud provider to alter specific files and exclude certain “average” customers, since doing so would undermine all customers in the system, even those considered “important” and, thus, profitable. Therefore, entangled storage schemes offer security “before-the-fact”.

  18. Silo Storage Preconceptual Design

    Energy Technology Data Exchange (ETDEWEB)

    Stephanie L. Austad; Patrick W. Bragassa; Kevin M Croft; David S Ferguson; Scott C Gladson; Annette L Shafer; John H Weathersby

    2012-09-01

    The National Nuclear Security Administration (NNSA) has a need to develop and field a low-cost option for the long-term storage of a variety of radiological material. The storage option’s primary requirement is to provide both environmental and physical protection of the materials. Design criteria for this effort require a low initial cost and minimum maintenance over a 50-year design life. In 1999, Argonne National Laboratory-West was tasked with developing a dry silo storage option for the BN-350 Spent Fuel in Aktau Kazakhstan. Argon’s design consisted of a carbon steel cylinder approximately 16 ft long, 18 in. outside diameter and 0.375 in. wall thickness. The carbon steel silo was protected from corrosion by a duplex coating system consisting of zinc and epoxy. Although the study indicated that the duplex coating design would provide a design life well in excess of the required 50 years, the review board was concerned because of the novelty of the design and the lack of historical use. In 2012, NNSA tasked Idaho National Laboratory (INL) with reinvestigating the silo storage concept and development of alternative corrosion protection strategies. The 2012 study, “Silo Storage Concepts, Cathodic Protection Options Study” (INL/EST-12-26627), concludes that the option which best fits the design criterion is a passive cathotic protection scheme, consisting of a carbon steel tube coated with zinc or a zinc-aluminum alloy encapsulated in either concrete or a cement grout. The hot dipped zinc coating option was considered most efficient, but the flame-sprayed option could be used if a thicker zinc coating was determined to be necessary.

  19. Underground Storage Tanks in Iowa

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — Underground storage tank (UST) sites which store petroleum in Iowa. Includes sites which have been reported to DNR, and have active or removed underground storage...

  20. Hydrogen Storage Technical Team Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-06-01

    The mission of the Hydrogen Storage Technical Team is to accelerate research and innovation that will lead to commercially viable hydrogen-storage technologies that meet the U.S. DRIVE Partnership goals.

  1. Radiatioactive Contamination in Storage Vaults

    International Science & Technology Center (ISTC)

    Determination of Radioactive Contamination and Conditions for Radioactive Wastes Storage in Interim Storage Ficilities (Vaults) of the Siberian Group of Chemical Enterprises (SGCE) for Substantiation of Measures for Safe Decommissioning of Plutonium-Production Reactors (PUGR)

  2. Carbon Capture and Geologic Storage

    International Nuclear Information System (INIS)

    This paper will briefly discuss carbon capture and storage options, mechanisms and costs. Risks from geologic storage risks will be addressed and the need for monitoring. Some current field studies will be described.

  3. Phosphonium chloride for thermal storage

    Science.gov (United States)

    Sutton, J. G.; Heimlich, P. F.; Tepper, E. H.

    1972-01-01

    Development of systems for storage of thermal energy is discussed. Application of phosphonium chloride for heat storage through reversible dissociation is described. Chemical, physical, and thermodynamic properties of phosphonium chloride are analyzed and dangers in using phosphonium chloride are explained.

  4. Mass storage for microprocessor farms

    International Nuclear Information System (INIS)

    Experiments in high energy physics require high density and high speed mass storage. Mass storage is needed for data logging during the online data acquisition, data retrieval and storage during the event reconstruction and data manipulation during the physics analysis. This paper examines the storage and speed requirements at the first two stages of the experiments and suggests a possible starting point to deal with the problem. 3 refs., 3 figs

  5. Modificaçoes bioquímicas e físicas em grãos de feijão durante o armazenamento Biochemical and physical modifications of bean seeds during storage

    Directory of Open Access Journals (Sweden)

    Haiko Enok Sawazaki

    1985-01-01

    Full Text Available Procurou-se estudar parâmetros químicos e físicos que pudessem avaliar, em três épocas, a qualidade de grãos de feijão durante onze meses de armazenamento, utilizando os cultivares Carioca e Rico 23. Eletroforese das frações globulinas em gel de poliacrilamida com sulfato dodecil sódico (SDS, indicou um aumento no final do número de bandas, porém o fato poderia ser conseqüência de uma maior concentração protéica da amostra, sendo praticamente mantidos durante o estudo os pesos moleculares das subunidades polipeptídicas principais das proteínas G1 e G2, de cerca de 50.000-43.000 e 18.000 respectivamente. As porcentagens de proteína, umidade e açúcar solúvel não sofreram alterações sensíveis, enquanto a de lipídio diminuiu e a de fibra aumentou. O teor de amido também aumentou, provavelmente em vista da maior extratibilidade. O índice de peróxido e o teor de ácidos graxos livres aumentaram, sendo comprovada, por este último, a elevação do índice de acidez. A capacidade de hidratação aumentou inicialmente, permanecendo depois constante, e a porcentagem de sementes com tegumento duro não pareceu sofrer alteração. O comportamento dos dois cultivares foi semelhante.Seeds of dry beans cvs Rico 23 and Carioca, were chemical and physically characterized three times during eleven months of storage. Electrophoresis in polyacrylamide gel with SDS of the globulin fraction showed an increase in the number of bands at the third sampling, probably due to a more efficient protein extraction. However, molecular weight of the G1 and G2 subunits were constant, about 50,000 -43,000 and 18,000, respectively. Total protein, soluble sugars and water contents did not change during storage, while fiber increased and lipids decreased. Starch content, as in case of globulin fraction, also increased due to greater extractability. Both free fatty acids content and peroxide value increased, according to the increase of the acidity value. Water absorption capability increased initially and leveled off thereafter. The percentage of seeds with hard coat was not affected by storage. The behaviour of the two cultivars was very similar.

  6. A Review of Encryption Storage

    OpenAIRE

    Chaowen Chang; Min Liang; Hongzhao Kou; Zhigang Si

    2010-01-01

    Information leakage leads to very serious consequences. Encryption storage is an effective means of ensuring information security. We summarize several typical encryption storage methods: application layer encryption, filter driver encryption, virtual disk encryption and full disk encryption. At last this paper compares these methods in some respects and provides the references for design and research of encryption storage systems.

  7. Storage for spherical fuel elements

    International Nuclear Information System (INIS)

    A storage for spherical fuel elements of nuclear reactors is equipped in a way, that the subcriticality of the storage is ensured even after complete discharge of the reactor core. The storage comprises at least one cylindric shaft which, apart from the fuel elements, absorber balls are filled in continuously in a pre-definable mixture ratio. (orig.)

  8. Alternatives for water basin spent fuel storage using pin storage

    International Nuclear Information System (INIS)

    The densest tolerable form for storing spent nuclear fuel is storage of only the fuel rods. This eliminates the space between the fuel rods and frees the hardware to be treated as non-fuel waste. The storage density can be as much as 1.07 MTU/ft2 when racks are used that just satisfy the criticality and thermal limitations. One of the major advantages of pin storage is that it is compatible with existing racks; however, this reduces the storage density to 0.69 MTU/ft2. Even this is a substantial increase over the 0.39 MTU/ft2 that is achievable with current high capacity stainless steel racks which have been selected as the bases for comparison. Disassembly requires extensive operation on the fuel assembly to remove the upper end fitting and to extract the fuel rods from the assembly skeleton. These operations will be performed with the aid of an elevator to raise the assembly where each fuel rod is grappled. Lowering the elevator will free the fuel rod for transfer to the storage canister. A storage savings of $1510 per MTU can be realized if the pin storage concept is incorporated at a new away-from-reactor facility. The storage cost ranges from $3340 to $7820 per MTU of fuel stored with the lower cost applying to storage at an existing away-from-reactor storage facility and the higher cost applying to at-reactor storage

  9. Natural gas storage

    International Nuclear Information System (INIS)

    Storage is an important link in the natural gas supply chain. It is a valuable aid to providing the link between remote sources of production and consumption locations. After a brief recall covering the role of storage this article describes the various options for storing natural gas: saline cavities, aquifers, liquefied gas tanks or artificial cavities. A report follows on the different solutions adopted in Europe and in Switzerland, more particularly that chosen by Gaznat in order to contribute to securing supplies in Western Switzerland. Market deregulation has brought changes to the regulations, which are in turn modifying the rules that were in force until just recently, and new topics are arising, such as strategic reserves and pricing rules for stored natural gas. (author)

  10. Entangled Cloud Storage

    DEFF Research Database (Denmark)

    Ateniese, Giuseppe; Dagdelen, Özgür; Damgård, Ivan Bjerre; Venturi, Daniele

    2012-01-01

    Entangled cloud storage enables a set of clients {P_i} to “entangle” their files {f_i} into a single clew c to be stored by a (potentially malicious) cloud provider S. The entanglement makes it impossible to modify or delete significant part of the clew without affecting all files in c. A clew...... keeps the files in it private but still lets each client P_i recover his own data by interacting with S; no cooperation from other clients is needed. At the same time, the cloud provider is discouraged from altering or overwriting any significant part of c as this will imply that none of the clients can...... recover their files. We provide theoretical foundations for entangled cloud storage, introducing the notion of an entangled encoding scheme that guarantees strong security requirements capturing the properties above. We also give a concrete construction based on privacy-preserving polynomial interpolation...

  11. Flexible Secure Cloud Storage

    OpenAIRE

    Graf, Sebastian

    2014-01-01

    Our life without Internet-based services is hard to imagine: We search for information with Google, share thoughts on Facebook, buy at Amazon and store our pictures on Flickr. Many of these Internet-based services focus on easy exchange of information, providing comfortable and ubiquitous storage and sharing. Relieved from hardware purchases, software bug fixes and infrastructure maintenance, users as well as companies use these cloud-based stores either for free or at low-cost. The price is ...

  12. Muon Storage Rings

    Energy Technology Data Exchange (ETDEWEB)

    Blondel, A. [LPNHE Ecole Polytechnique, Palaiseau (France)

    1999-06-01

    Muon storage rings are reviewed in an introductory manner. The physics opportunities are exciting. The technical difficulties are considerable, but can be spread over three step scenario. First a neutrino factory would make definitive experiments on neutrino oscillations; then several generations of precision muon colliders could explore the spectrum of particles associated with electroweak symmetry breaking or supersymmetry; finally high energy lepton collisions could be envisaged. (author) 32 refs, 10 figs, 1 tab

  13. Muon Storage Rings

    International Nuclear Information System (INIS)

    Muon storage rings are reviewed in an introductory manner. The physics opportunities are exciting. The technical difficulties are considerable, but can be spread over three step scenario. First a neutrino factory would make definitive experiments on neutrino oscillations; then several generations of precision muon colliders could explore the spectrum of particles associated with electroweak symmetry breaking or supersymmetry; finally high energy lepton collisions could be envisaged. (author)

  14. ENERGY STORAGE SYSTEMS

    OpenAIRE

    ELENA RADUCAN; LUMINITA MORARU

    2011-01-01

    In this paper you will find an overview of systems and energy storage techniques and a comparison of the important characteristics of them. Delocalized electricity production and the introduction of variable, fluctuating sources (renewable energy: solar, wind turbines, etc.) increase the difficulty of stabilizing the power network, mainly due to a supply-demand imbalance. It is therefore convenient to generate the energy, transmit it, convert it, and then store it if need be. More than ever t...

  15. Storage ring group summary

    International Nuclear Information System (INIS)

    The Storage Ring Group set out to identify and pursue salient problems in accelerator physics for heavy ion fusion, divorced from any particular reference design concept. However, it became apparent that some basic parameter framework was required to correlate the different study topics. As the Workshop progressed, ring parameters were modified and updated. Consequently, the accompanying papers on individual topics will be found to refer to slightly varied parameters, according to the stage at which the different problems were tackled

  16. Radioactive waste storage containers

    International Nuclear Information System (INIS)

    Purpose: To obtain storage containers with no destruction risks caused by external temperature changes and requiring neither cooling facility nor running cost. Constitution: Heat dissipation walls are disposed in adjacent with the inside of a concrete container. The heat dissipation walls constitute a vacuum container in which heat pipes having operation fluids sealed therein are disposed. Liquids having heat transfer function due to convection are charged in a space surrounded with the heat dissipation walls in which radioactive wastes are contained. (Ikeda, J.)

  17. Chemical energy storage

    CERN Document Server

    Schlögl, Robert; Antonietti, Markus; Arndt, Sebastian; Behrens, Malte; Bill, Eckhard; Brandner, Armin; Centi, Gabriele; Claus, Peter; Cox, Nicholas; DeBeer, Serena; DeMartini, Nikolai; Doblhofer, Karl; Franzke, Thomas; Freund, Hans-Joachim; Gastel, Maurice; Grunwaldt, Jan-Dierk; Hofmann, Gerhard; Hupa, Mikko; Kähler, Kevin; Kunkes, Edward; Loosdrecht, Jan; Lubitz, Wolfgang; Maier, Joachim; Menzel, Dietrich; Muhler, Martin; Murzin, Dmitry Yu; Neese, Frank; Niemantsverdriet, J W (Hans); Nilius, Niklas; Palkovits, Regina; Pantazis, Dimitrios A; Perathoner, Siglinda; Petrenko, Taras; Rossmeisl, Jan; Samuelis, Dominik; Schlögl, Robert; Schomäcker, Reinhard; Schüth, Ferdi; Shaikhutdinov, Shamil; Sterrer, Martin; Strasser, Peter; Trunschke, Annette; Wright, William R H; Ye, Shengfa

    2012-01-01

    Energy - in the headlines, discussed controversially, vital. The use of regenerative energy in many primary forms leads to the necessity to store grid dimensions for maintaining continuous supply and enabling the replacement of fossil fuel systems. This work provides a hands-on insight into the present status of energy conversion and deals with aspects of chemical energy storage considering the geosphere, electrochemistry, catalysis, synthesis of catalysts, functional analysis of catalytic processes and the interface between electrochemistry and heterogeneous catalysis.

  18. DPM: Future Proof Storage

    International Nuclear Information System (INIS)

    The Disk Pool Manager (DPM) is a lightweight solution for grid enabled disk storage management. Operated at more than 240 sites it has the widest distribution of all grid storage solutions in the WLCG infrastructure. It provides an easy way to manage and configure disk pools, and exposes multiple interfaces for data access (rfio, xroot, nfs, gridftp and http/dav) and control (srm). During the last year we have been working on providing stable, high performant data access to our storage system using standard protocols, while extending the storage management functionality and adapting both configuration and deployment procedures to reuse commonly used building blocks. In this contribution we cover in detail the extensive evaluation we have performed of our new HTTP/WebDAV and NFS 4.1 frontends, in terms of functionality and performance. We summarize the issues we faced and the solutions we developed to turn them into valid alternatives to the existing grid protocols - namely the additional work required to provide multi-stream transfers for high performance wide area access, support for third party copies, credential delegation or the required changes in the experiment and fabric management frameworks and tools. We describe new functionality that has been added to ease system administration, such as different filesystem weights and a faster disk drain, and new configuration and monitoring solutions based on the industry standards Puppet and Nagios. Finally, we explain some of the internal changes we had to do in the DPM architecture to better handle the additional load from the analysis use cases.

  19. Energy storage arrangement

    International Nuclear Information System (INIS)

    This patent describes a method for energy storage in which energy is stored by compression of a metastable degenerate Fermi electron gas subjected to a magnetic field. It comprises subjecting a material to a very high pressure in a diamond anvil high pressure cell to form a metallic state of the material containing a metastable degenerate Fermi electron gas, and applying a magnetic field to the metastable degenerate Fermi electron gas to produce a compression of the metastable degenerate Fermi electron gas therein

  20. Energy Storage Project

    Science.gov (United States)

    Mercer, Carolyn R.; Jankovsky, Amy L.; Reid, Concha M.; Miller, Thomas B.; Hoberecht, Mark A.

    2011-01-01

    NASA's Exploration Technology Development Program funded the Energy Storage Project to develop battery and fuel cell technology to meet the expected energy storage needs of the Constellation Program for human exploration. Technology needs were determined by architecture studies and risk assessments conducted by the Constellation Program, focused on a mission for a long-duration lunar outpost. Critical energy storage needs were identified as batteries for EVA suits, surface mobility systems, and a lander ascent stage; fuel cells for the lander and mobility systems; and a regenerative fuel cell for surface power. To address these needs, the Energy Storage Project developed advanced lithium-ion battery technology, targeting cell-level safety and very high specific energy and energy density. Key accomplishments include the development of silicon composite anodes, lithiated-mixed-metal-oxide cathodes, low-flammability electrolytes, and cell-incorporated safety devices that promise to substantially improve battery performance while providing a high level of safety. The project also developed "non-flow-through" proton-exchange-membrane fuel cell stacks. The primary advantage of this technology set is the reduction of ancillary parts in the balance-of-plant--fewer pumps, separators and related components should result in fewer failure modes and hence a higher probability of achieving very reliable operation, and reduced parasitic power losses enable smaller reactant tanks and therefore systems with lower mass and volume. Key accomplishments include the fabrication and testing of several robust, small-scale nonflow-through fuel cell stacks that have demonstrated proof-of-concept. This report summarizes the project s goals, objectives, technical accomplishments, and risk assessments. A bibliography spanning the life of the project is also included.

  1. Linear storage projector

    International Nuclear Information System (INIS)

    A description is given of a radiographic apparatus for manipulating a quantity of radioactive material between a stored position and a use position including a capsule of said radioactive material, a storage unit with means defining a passage through it, for storing the capsule in the passage and shielding the surrounding environment from the stored radioactive material, and manipulating means connectible to said storage unit at a first end of said passage for moving said capsule between a stored position within the passage and a use position outside the second end of said passage, the improvement comprising: a shutter mounted on said storage unit for slidng movement transverse to the second end of said passage between first and second limits, said shutter in said first limit blocking said second end, said shutter having a hole through it which registers with said passage when the shutter is in said second limit, shutter-retaining means associated with said passage means adjacent said second end and resilient means cooperating with said retaining means and said storage unit for urging said retaining means to project an end-part toward said shutter, means in said shutter for receiving said end-part when said shutter is in said second limit and thereby retaining said hole in register with said passage, and means coupled to said capsule for pulling said retaining means away from said shutter against the action of said resilient means under control of said manipulating means for withdrawing said end-part from said receiving means and thereby permitting said shutter to move toward said first limit

  2. Tuber Storage Proteins

    OpenAIRE

    Peter R. Shewry

    2003-01-01

    A wide range of plants are grown for their edible tubers, but five species together account for almost 90 % of the total world production. These are potato (Solanum tuberosum), cassava (Manihot esculenta), sweet potato (Ipomoea batatus), yams (Dioscorea spp.) and taro (Colocasia, Cyrtosperma and Xanthosoma spp.). All of these, except cassava, contain groups of storage proteins, but these differ in the biological properties and evolutionary relationships. Thus, patatin from potato exhibits act...

  3. Magnesium for Hydrogen Storage

    DEFF Research Database (Denmark)

    Pedersen, Allan Schrøder; Kjøller, John; Larsen, B.; Vigeholm, B.

    1983-01-01

    A study of the hydrogenation characteristics of fine magnesium powder during repeated cycling has been performed using a high-pressure microbalance facility. No effect was found from the cycling regarding kinetics and storage capacity. The reaction rate of the absorption process was fast at temperatures around 600 K and above, but the reversed reaction showed somewhat slower kinetics around 600 K. At higher temperatures the opposite was found. The enthalpy and entropy change by the hydrogenation...

  4. Holographic content addressable storage

    Science.gov (United States)

    Chao, Tien-Hsin; Lu, Thomas; Reyes, George

    2015-03-01

    We have developed a Holographic Content Addressable Storage (HCAS) architecture. The HCAS systems consists of a DMD (Digital Micromirror Array) as the input Spatial Light Modulator (SLM), a CMOS (Complementary Metal-oxide Semiconductor) sensor as the output photodetector and a photorefractive crystal as the recording media. The HCAS system is capable of performing optical correlation of an input image/feature against massive reference data set stored in the holographic memory. Detailed system analysis will be reported in this paper.

  5. SASE FEL Storage Ring

    CERN Document Server

    Dattoli, G; Petralia, A; Rau, J V

    2012-01-01

    We explore the possibility of operating a SASE FEL with a Storage Ring. We use a semi-analytical model to obtain the evolution inside the undulator by taking into account the interplay on the laser dynamics due to the induced energy spread and to the radiation damping. We obtain the Renieri's limit for the stationary output power and discuss the possibility of including in our model the effect of the beam instabilities.

  6. Maui energy storage study.

    Energy Technology Data Exchange (ETDEWEB)

    Ellison, James; Bhatnagar, Dhruv; Karlson, Benjamin

    2012-12-01

    This report investigates strategies to mitigate anticipated wind energy curtailment on Maui, with a focus on grid-level energy storage technology. The study team developed an hourly production cost model of the Maui Electric Company (MECO) system, with an expected 72 MW of wind generation and 15 MW of distributed photovoltaic (PV) generation in 2015, and used this model to investigate strategies that mitigate wind energy curtailment. It was found that storage projects can reduce both wind curtailment and the annual cost of producing power, and can do so in a cost-effective manner. Most of the savings achieved in these scenarios are not from replacing constant-cost diesel-fired generation with wind generation. Instead, the savings are achieved by the more efficient operation of the conventional units of the system. Using additional storage for spinning reserve enables the system to decrease the amount of spinning reserve provided by single-cycle units. This decreases the amount of generation from these units, which are often operated at their least efficient point (at minimum load). At the same time, the amount of spinning reserve from the efficient combined-cycle units also decreases, allowing these units to operate at higher, more efficient levels.

  7. Mass storage at NSA

    Science.gov (United States)

    Shields, Michael F.

    1993-01-01

    The need to manage large amounts of data on robotically controlled devices has been critical to the mission of this Agency for many years. In many respects this Agency has helped pioneer, with their industry counterparts, the development of a number of products long before these systems became commercially available. Numerous attempts have been made to field both robotically controlled tape and optical disk technology and systems to satisfy our tertiary storage needs. Custom developed products were architected, designed, and developed without vendor partners over the past two decades to field workable systems to handle our ever increasing storage requirements. Many of the attendees of this symposium are familiar with some of the older products, such as: the Braegen Automated Tape Libraries (ATL's), the IBM 3850, the Ampex TeraStore, just to name a few. In addition, we embarked on an in-house development of a shared disk input/output support processor to manage our every increasing tape storage needs. For all intents and purposes, this system was a file server by current definitions which used CDC Cyber computers as the control processors. It served us well and was just recently removed from production usage.

  8. Spent fuel storage chamber

    International Nuclear Information System (INIS)

    In a dry spent nuclear fuel storage chamber, an atmosphere in a closed loop comprising storage cell/heated air collecting chamber/cooling air circulation path is filled with gases having a high thermal radiation absorbing performance. Heat released from the spent fuels heats a cylindrical vessel, gases in contact with the peripheral surface thereof and metal blocks constituting the storage cell. Since the gases having highly heat absorbing performance are filled, they are heated by absorbing radiation heat of the spent fuels, to improve the heat dissipation efficiency of the spent fuels. Accordingly, even if the heat generation amount of the spent fuels is great, the temperature elevation can be suppressed since the heat dissipation efficiency of the spent fuels is great due to radiation absorption. In addition, a phenomenon that the temperature of the cylindrical vessel is raised can be suppressed. As a result, fuels or mixed oxide fuels of a high burnup degree having greater heat generation amount compared with usual fuels can be stored safely and economically. (N.H.)

  9. Linear capacity storage devices

    International Nuclear Information System (INIS)

    The linearity of information storage in capacitor storage circuits is discussed. The paper describes two storage circuits whose operation is linear over a wide range of output-signal amplitudes. In one of the circuits the error signal is measured by a differential amplifier and, thanks to the high amplification factor of the amplifier, good linearity is obtained. The second circuit was designed for work in the nanosecond pulse-width range. Input pulses of standard width cut off a pentode, in the anode of which there is a memory capacitor. Since the capacitor is charged from the anode supply through a current generator, the charging current remains almost constant during measurement of the capacitor voltage. The pentode has a large internal resistance so that after the end of the input pulse the magnitude of the current through the pentode is almost equal to its initial value, and the charge stored by the capacitor is remembered. The width of the information memory is limited by the output resistance of the current generator and the internal resistance (Ri) of the pentode. To increase the memory width, a diode may be inserted before the capacitor. (author)

  10. Scalable cloud without dedicated storage

    Science.gov (United States)

    Batkovich, D. V.; Kompaniets, M. V.; Zarochentsev, A. K.

    2015-05-01

    We present a prototype of a scalable computing cloud. It is intended to be deployed on the basis of a cluster without the separate dedicated storage. The dedicated storage is replaced by the distributed software storage. In addition, all cluster nodes are used both as computing nodes and as storage nodes. This solution increases utilization of the cluster resources as well as improves fault tolerance and performance of the distributed storage. Another advantage of this solution is high scalability with a relatively low initial and maintenance cost. The solution is built on the basis of the open source components like OpenStack, CEPH, etc.

  11. Personal storage for mobile applications

    Science.gov (United States)

    Coughlin, Thomas M.

    Vast and inexpensive digital storage provided by hard disk drives, flash memory and other storage devices has changed the face of mobile consumer electronics over the last 10 years and will continue to do so in the future. Elements of the mobile digital storage hierarchy can be combined together to create combinations capable of more than either device by itself. By integrating applications into storage device more cost effective, higher performance and more reliable devices may be constructed. Sharing of content will create much greater demand for storage since a shared file can be multiplied many times over. Likewise demand for higher resolution content and for capturing ever greater details of the life of family members will drive increases in commercial as well as personal content storage demand. The growth of storage in and around the home will require an integrated storage network for managing, backing up and indexing all of this content. If such an integrated storage utility can be put into most homes there will be a huge demand for storage in consumer devices and in the local and remote networks that support the retention of this content. This demand will drive the proliferation storage devices such as hard disk drives.

  12. Fuel performance in water storage

    International Nuclear Information System (INIS)

    Westinghouse Idaho Nuclear Company operates the Idaho Chemical Processing Plant (ICPP) at the Idaho National Engineering Laboratory (INEL) for the Department of Energy (DOE). A variety of different types of fuels have been stored there since the 1950's prior to reprocessing for uranium recovery. In April of 1992, the DOE decided to end fuel reprocessing, changing the mission at ICPP. Fuel integrity in storage is now viewed as long term until final disposition is defined and implemented. Thus, the condition of fuel and storage equipment is being closely monitored and evaluated to ensure continued safe storage. There are four main areas of fuel storage at ICPP: an original underwater storage facility (CPP-603), a modern underwater storage facility (CPP-666), and two dry fuel storage facilities. The fuels in storage are from the US Navy, DOE (and its predecessors the Energy Research and Development Administration and the Atomic Energy Commission), and other research programs. Fuel matrices include uranium oxide, hydride, carbide, metal, and alloy fuels. In the underwater storage basins, fuels are clad with stainless steel, zirconium, and aluminum. Also included in the basin inventory is canned scrap material. The dry fuel storage contains primarily graphite and aluminum type fuels. A total of 55 different fuel types are currently stored at the Idaho Chemical Processing Plant. The corrosion resistance of the barrier material is of primary concern in evaluating the integrity of the fuel in long term water storage. The barrier material is either the fuel cladding (if not canned) or the can material

  13. Dry storage of Magnox fuel

    International Nuclear Information System (INIS)

    This work, commissioned by the CEGB, studies the feasibility of a combination of short-term pond storage and long-term dry storage of Magnox spent fuel as a cheaper alternative to reprocessing. Storage would be either at the reactor site or a central site. Two designs are considered, based on existing design work done by GEC-ESL and NNC; the capsule design developed by NNC and with storage in passive vaults for up to 100 yrs and the GEC-ESL tube design developed at Wylfa for the interim storage of LWR. For the long-term storage of Magnox spent fuel the GEC-ESL tubed vault all-dry storage method is recommended and specifications for this method are given. (U.K.)

  14. Moving Towards SAN Storage: An Enterprise Perspective

    OpenAIRE

    rabi prasad padhy

    2012-01-01

    Abstract – One of the major components of any enterprise IT infrastructure is data storage. There has been continuous effort to explore new storage technologies to improve performance with respect to input/output operations, data availability, consolidation of storage assets and data protection and archiving. Storage has moved from the traditional Direct Attached Storage (DAS) to Network Attached Storage (NAS) and now to Storage Area Networks (SAN). Traditional storage subsystems like Server ...

  15. Holographic Optical Data Storage

    Science.gov (United States)

    Timucin, Dogan A.; Downie, John D.; Norvig, Peter (Technical Monitor)

    2000-01-01

    Although the basic idea may be traced back to the earlier X-ray diffraction studies of Sir W. L. Bragg, the holographic method as we know it was invented by D. Gabor in 1948 as a two-step lensless imaging technique to enhance the resolution of electron microscopy, for which he received the 1971 Nobel Prize in physics. The distinctive feature of holography is the recording of the object phase variations that carry the depth information, which is lost in conventional photography where only the intensity (= squared amplitude) distribution of an object is captured. Since all photosensitive media necessarily respond to the intensity incident upon them, an ingenious way had to be found to convert object phase into intensity variations, and Gabor achieved this by introducing a coherent reference wave along with the object wave during exposure. Gabor's in-line recording scheme, however, required the object in question to be largely transmissive, and could provide only marginal image quality due to unwanted terms simultaneously reconstructed along with the desired wavefront. Further handicapped by the lack of a strong coherent light source, optical holography thus seemed fated to remain just another scientific curiosity, until the field was revolutionized in the early 1960s by some major breakthroughs: the proposition and demonstration of the laser principle, the introduction of off-axis holography, and the invention of volume holography. Consequently, the remainder of that decade saw an exponential growth in research on theory, practice, and applications of holography. Today, holography not only boasts a wide variety of scientific and technical applications (e.g., holographic interferometry for strain, vibration, and flow analysis, microscopy and high-resolution imagery, imaging through distorting media, optical interconnects, holographic optical elements, optical neural networks, three-dimensional displays, data storage, etc.), but has become a prominent am advertising, and security medium as well. The evolution of holographic optical memories has followed a path not altogether different from holography itself, with several cycles of alternating interest over the past four decades. P. J. van Heerden is widely credited for being the first to elucidate the principles behind holographic data storage in a 1963 paper, predicting bit storage densities on the order of 1/lambda(sup 3) with source wavelength lambda - a fantastic capacity of nearly 1 TB/cu cm for visible light! The science and engineering of such a storage paradigm was heavily pursued thereafter, resulting in many novel hologram multiplexing techniques for dense data storage, as well as important advances in holographic recording materials. Ultimately, however, the lack of such enabling technologies as compact laser sources and high performance optical data I/O devices dampened the hopes for the development of a commercial product. After a period of relative dormancy, successful applications of holography in other arenas sparked a renewed interest in holographic data storage in the late 1980s and the early 1990s. Currently, with most of the critical optoelectronic device technologies in place and the quest for an ideal holographic recording medium intensified, holography is once again considered as one of several future data storage paradigms that may answer our constantly growing need for higher-capacity and faster-access memories.

  16. Storage Management and Data Acquisition

    Directory of Open Access Journals (Sweden)

    Kavya Bhat

    2013-06-01

    Full Text Available Enterprise storage is the computer data storage designed for large-scale, high-technology environments of the modern enterprises which is very time efficient where in stored data can be accessed in less time. When comparing to the consumer storage, it has higher scalability, higher reliability and better fault tolerance. As well, criticality of data varies between enterprises. Challenges faced in current scenario to store big data are in terms of cost, data loss, efficiency while accessing data, maintaining consistency of data and many more. In order to provide better storage solution and data management, the proposed solution came up with platform of Workflow Automation (WFA. WFA is an active management tool which directly allocates storage on storage server based on client request. It depends on a data source i.e., OnCommand Unified Manager (OCUM to monitor the storage components. OCUM acts as a passive reporting tool, which polls all the storage data at different time stamps. The monitored data includes parameters and attributes of storage component like corrupted disk data, normal disk data or may be some lack of storage space. WFA has cache based intelligence and it acquires only relevant data of context from OCUM. Based on this acquired cache data, WFA can provide better storage solutions and data management by which it takes care of conditions like maintaining health of storage and takes appropriate actions like migrating data, replacing corrupted disk etc., The acquired cache data can be queried by filter/ finders to select storage component as a resource on which data is stored. The results of which will work on selective resource, to execute specific task of interest using workflows. Query results return the count of storage components and related information to verify consistency and no data loss from any storage resource. Hence the proposed solution helps in performance tuning of big data storage solutions in terms of data access time, reliability, efficiency, data consistency and security. It reduces the cost of managing storage, enables adherence to best practices for storage processes.

  17. Gas Storage Technology Consortium

    Energy Technology Data Exchange (ETDEWEB)

    Joel Morrison; Elizabeth Wood; Barbara Robuck

    2010-09-30

    The EMS Energy Institute at The Pennsylvania State University (Penn State) has managed the Gas Storage Technology Consortium (GSTC) since its inception in 2003. The GSTC infrastructure provided a means to accomplish industry-driven research and development designed to enhance the operational flexibility and deliverability of the nation's gas storage system, and provide a cost-effective, safe, and reliable supply of natural gas to meet domestic demand. The GSTC received base funding from the U.S. Department of Energy's (DOE) National Energy Technology Laboratory (NETL) Oil & Natural Gas Supply Program. The GSTC base funds were highly leveraged with industry funding for individual projects. Since its inception, the GSTC has engaged 67 members. The GSTC membership base was diverse, coming from 19 states, the District of Columbia, and Canada. The membership was comprised of natural gas storage field operators, service companies, industry consultants, industry trade organizations, and academia. The GSTC organized and hosted a total of 18 meetings since 2003. Of these, 8 meetings were held to review, discuss, and select proposals submitted for funding consideration. The GSTC reviewed a total of 75 proposals and committed co-funding to support 31 industry-driven projects. The GSTC committed co-funding to 41.3% of the proposals that it received and reviewed. The 31 projects had a total project value of $6,203,071 of which the GSTC committed $3,205,978 in co-funding. The committed GSTC project funding represented an average program cost share of 51.7%. Project applicants provided an average program cost share of 48.3%. In addition to the GSTC co-funding, the consortium provided the domestic natural gas storage industry with a technology transfer and outreach infrastructure. The technology transfer and outreach were conducted by having project mentoring teams and a GSTC website, and by working closely with the Pipeline Research Council International (PRCI) to jointly host technology transfer meetings and occasional field excursions. A total of 15 technology transfer/strategic planning workshops were held.

  18. Energy Storage System

    Science.gov (United States)

    1996-01-01

    SatCon Technology Corporation developed the drive train for use in the Chrysler Corporation's Patriot Mark II, which includes the Flywheel Energy Storage (FES) system. In Chrysler's experimental hybrid- electric car, the hybrid drive train uses an advanced turboalternator that generates electricity by burning a fuel; a powerful, compact electric motor; and a FES that eliminates the need for conventional batteries. The FES system incorporates technology SatCon developed in more than 30 projects with seven NASA centers, mostly for FES systems for spacecraft attitude control and momentum recovery. SatCon will continue to develop the technology with Westinghouse Electric Corporation.

  19. Materials for hydrogen storage

    Directory of Open Access Journals (Sweden)

    Andreas Züttel

    2003-09-01

    The goal is to pack hydrogen as close as possible, i.e. to reach the highest volumetric density by using as little additional material as possible. Hydrogen storage implies the reduction of an enormous volume of hydrogen gas. At ambient temperature and atmospheric pressure, 1 kg of the gas has a volume of 11 m3. To increase hydrogen density, work must either be applied to compress the gas, the temperature decreased below the critical temperature, or the repulsion reduced by the interaction of hydrogen with another material.

  20. Regulated underground storage tanks

    International Nuclear Information System (INIS)

    This guidance package is designed to assist DOE Field operations by providing thorough guidance on the underground storage tank (UST) regulations. [40 CFR 280]. The guidance uses tables, flowcharts, and checklists to provide a ''roadmap'' for DOE staff who are responsible for supervising UST operations. This package is tailored to address the issues facing DOE facilities. DOE staff should use this guidance as: An overview of the regulations for UST installation and operation; a comprehensive step-by-step guidance for the process of owning and operating an UST, from installation to closure; and a quick, ready-reference guide for any specific topic concerning UST ownership or operation

  1. Solar energy storage

    CERN Document Server

    Sorensen, Bent

    2015-01-01

    While solar is the fastest-growing energy source in the world, key concerns around solar power's inherent variability threaten to de-rail that scale-up . Currently, integration of intermittent solar resources into the grid creates added complication to load management, leading some utilities to reject it altogether, while other operators may penalize the producers via rate increases or force solar developers to include storage devices on-site to smooth out power delivery at the point of production. However these efforts at mitigation unfold, it is increasingly clear to parties on all sides th

  2. Magnesium for Hydrogen Storage

    DEFF Research Database (Denmark)

    Pedersen, Allan Schrøder; Kjøller, John; Larsen, B.; Vigeholm, B.

    1983-01-01

    A study of the hydrogenation characteristics of fine magnesium powder during repeated cycling has been performed using a high-pressure microbalance facility. No effect was found from the cycling regarding kinetics and storage capacity. The reaction rate of the absorption process was fast at...... temperatures around 600 K and above, but the reversed reaction showed somewhat slower kinetics around 600 K. At higher temperatures the opposite was found. The enthalpy and entropy change by the hydrogenation, derived from pressure-concentration isotherms, agree fairly well with those reported earlier....

  3. SALES, STORAGE AND SALVAGE

    CERN Multimedia

    Division SPL, groupe logistique; A. Notar

    2000-01-01

    From 3 January 2000 there will be a security barrier in front of the storage-recycling area in bldg 133, which will be accessible only to authorised staff and contractors.You are reminded that the equipment delivered to this area must be unpolluted and non-radioactive. The cost of recycling the equipment will be debited to the budget code of the Division concerned, with the prior approval of the Group Leader.Reminder relating to equipment salesThe Sales Section is open on Thursdays from 13.30 to 15.00 hours only.SPL DivisionLogistics GroupA. Notari

  4. Nuclear fuel storage arrangement

    International Nuclear Information System (INIS)

    An arrangement is disclosed for the storage of nuclear reactor fuel assemblies having a section wherein fuel is present and a section wherein fuel is not present. The fuel assemblies are placed in a plurality of elongated cells which are joined together to form a cellular structure. The fuel assemblies are placed within the cells at different elevations so that the fuel-containing section of one fuel assembly is next to the non-fuel-containing sections of each fuel assembly surrounding the first fuel assembly. The vertical staggering of the fuel-containing sections achieves space reductions while maintaining the stored fuel in a subcritical assemblage. 12 claims, 10 figures

  5. TEXT Energy Storage System

    International Nuclear Information System (INIS)

    The Texas Experimental Tokamak (TEXT) Enery Storage System, designed by the Center for Electromechanics (CEM), consists of four 50 MJ, 125 V homopolar generators and their auxiliaries and is designed to power the toroidal and poloidal field coils of TEXT on a two-minute duty cycle. The four 50 MJ generators connected in series were chosen because they represent the minimum cost configuration and also represent a minimal scale up from the successful 5.0 MJ homopolar generator designed, built, and operated by the CEM

  6. Interim storage report

    International Nuclear Information System (INIS)

    High-level radioactive waste (HLW) stored at the Idaho Chemical Processing Plant (ICPP) in the form of calcine and liquid and liquid sodium-bearing waste (SBW) will be processed to provide a stable waste form and prepare the waste to be transported to a permanent repository. Because a permanent repository will not be available when the waste is processed, the waste must be stored at ICPP in an Interim Storage Facility (ISF). This report documents consideration of an ISF for each of the waste processing options under consideration

  7. Interim storage study report

    Energy Technology Data Exchange (ETDEWEB)

    Rawlins, J.K.

    1998-02-01

    High-level radioactive waste (HLW) stored at the Idaho Chemical Processing Plant (ICPP) in the form of calcine and liquid and liquid sodium-bearing waste (SBW) will be processed to provide a stable waste form and prepare the waste to be transported to a permanent repository. Because a permanent repository will not be available when the waste is processed, the waste must be stored at ICPP in an Interim Storage Facility (ISF). This report documents consideration of an ISF for each of the waste processing options under consideration.

  8. Caracterización electroforética de las globulinas del grano fermentado de tres tipos de cacao / ELECTROPHORETIC CHARACTERIZATION OF GLOBULINS FROM FERMENTED GRAINS OF THREE TYPES OF COCOA / CARACTERIZAÇÃO ELETROFORÉTICA DAS GLOBULINAS DO GRÃO FERMENTADO DE TRES TIPOS DE CACAU

    Scientific Electronic Library Online (English)

    Ligia, Ortiz de Bertorelli; Henry, Maldonado; Pablo, Parra; Lucía, Graziani de Fariñas.

    2006-06-01

    Full Text Available Caracterizaram-se eletroforéticamente as globulinas do grão fermentado dos cacaus tipo crioulo, forasteiro e trinitário da localidade de Cumboto, estado Aragua, Venezuela, para o qual foram colhidas vagens de duas plantas por cada tipo de cacau, na razão de três frutos por planta, nos lotes El Paraí [...] so, La Vega de Santa Cruz e La Isleta. Vagens sãs de cada tipo de cacau foram processadas manualmente para retirada dos grãos e submetidas a um processo de fermentação durante 5 dias. A polpa dos grãos fermentados foi eliminada, e os cotilédones foram secados em uma estufa por 48h a 40°C, seguido de um processo de moenda. As globulinas foram extraídas com solução 0,5M NaCl e analisadas eletroforéticamente por PAGE-SDS unidimensional em gel de placa de poliacrilamida 20%. Os resultados revelaram variações significativas (p Abstract in spanish Se caracterizaron electroforéticamente las globulinas del grano fermentado de los cacaos tipo criollo, forastero y trinitario de la localidad de Cumboto, estado Aragua, Venezuela, para lo cual fueron cosechadas mazorcas de dos plantas por cada tipo de cacao, a razón de tres frutos por planta, en las [...] parcelas El Paraíso, La Vega de Santa Cruz y La Isleta. Mazorcas sanas de cada tipo de cacao fueron desgranadas manualmente y sometidas a un proceso de fermentación durante 5 días. A los granos fermentados se les eliminó la pulpa, y los cotiledones fueron secados en una estufa por 48h a 40ºC, seguido de un proceso de molienda. Las globulinas fueron extraídas con solución 0,5M NaCl y analizadas electroforéticamente por PAGE-SDS unidimensional en gel de placa de poliacrilamida 20%. Los resultados revelaron variaciones significativas (p Abstract in english The globulins of the fermented grain of the criollo, forastero and trinitario cocoa types in the Cumboto area, Aragua state, Venezuela were characterized by electrophoresis. Pods from two plants, three fruits per plant, were harvested for each type of cocoa in El Paraíso, La Vega de Santa Cruz and L [...] a Isleta parcels. Healthy pods corresponding to each type of cocoa were manually flailed and underwent a fermentation process for a period of five days. The pulp was removed from the fermented grains, and the cotiledons were dried in an oven for 48h at 40ºC. This was followed by a grinding process. The globulins were extracted using 0.5M NaCl and were analyzed by unidimensional PAGE-SDS electrophoresis in a 20% poliacrilamide plate gel. The results revealed significant variations (p

  9. Low temperature latent heat thermal energy storage - Heat storage materials

    Science.gov (United States)

    Abhat, A.

    1983-01-01

    Heat-of-fusion storage materials for low temperature latent heat storage in the temperature range 0-120 C are reviewed. Organic and inorganic heat storage materials classified as paraffins, fatty acids, inorganic salt hydrates and eutectic compounds are considered. The melting and freezing behavior of the various substances is investigated using the techniques of Thermal Analysis and Differential Scanning Calorimetry. The importance of thermal cycling tests for establishing the long-term stability of the storage materials is discussed. Finally, some data pertaining to the corrosion compatibility of heat-of-fusion substances with conventional materials of construction is presented.

  10. Probe-based data storage

    CERN Document Server

    Koelmans, Wabe W; Abelmann, L

    2015-01-01

    Probe-based data storage attracted many researchers from academia and industry, resulting in unprecendeted high data-density demonstrations. This topical review gives a comprehensive overview of the main contributions that led to the major accomplishments in probe-based data storage. The most investigated technologies are reviewed: topographic, phase-change, magnetic, ferroelectric and atomic and molecular storage. Also, the positioning of probes and recording media, the cantilever arrays and parallel readout of the arrays of cantilevers are discussed. This overview serves two purposes. First, it provides an overview for new researchers entering the field of probe storage, as probe storage seems to be the only way to achieve data storage at atomic densities. Secondly, there is an enormous wealth of invaluable findings that can also be applied to many other fields of nanoscale research such as probe-based nanolithography, 3D nanopatterning, solid-state memory technologies and ultrafast probe microscopy.

  11. Flywheel energy storage workshop

    Energy Technology Data Exchange (ETDEWEB)

    O`Kain, D.; Carmack, J. [comps.

    1995-12-31

    Since the November 1993 Flywheel Workshop, there has been a major surge of interest in Flywheel Energy Storage. Numerous flywheel programs have been funded by the Advanced Research Projects Agency (ARPA), by the Department of Energy (DOE) through the Hybrid Vehicle Program, and by private investment. Several new prototype systems have been built and are being tested. The operational performance characteristics of flywheel energy storage are being recognized as attractive for a number of potential applications. Programs are underway to develop flywheels for cars, buses, boats, trains, satellites, and for electric utility applications such as power quality, uninterruptible power supplies, and load leveling. With the tremendous amount of flywheel activity during the last two years, this workshop should again provide an excellent opportunity for presentation of new information. This workshop is jointly sponsored by ARPA and DOE to provide a review of the status of current flywheel programs and to provide a forum for presentation of new flywheel technology. Technology areas of interest include flywheel applications, flywheel systems, design, materials, fabrication, assembly, safety & containment, ball bearings, magnetic bearings, motor/generators, power electronics, mounting systems, test procedures, and systems integration. Information from the workshop will help guide ARPA & DOE planning for future flywheel programs. This document is comprised of detailed viewgraphs.

  12. Carbon capture and storage

    International Nuclear Information System (INIS)

    Carbon capture and storage (CCS) covers a broad range of technologies that are being developed to allow carbon dioxide (CO2) emissions from fossil fuel use at large point sources to be transported to safe geological storage, rather than being emitted to the atmosphere. Some key enabling contributions from technology development that could help to facilitate the widespread commercial deployment of CCS are expected to include cost reductions for CO2 capture technology and improved techniques for monitoring stored CO2. It is important, however, to realise that CCS will always require additional energy compared to projects without CCS, so will not be used unless project operators see an appropriate value for reducing CO2 emissions from their operations or legislation is introduced that requires CCS to be used. Possible key advances for CO2 capture technology over the next 50 years, which are expected to arise from an eventual adoption of CCS as standard practice for all large stationary fossil fuel installations, are also identified. These include continued incremental improvements (e.g. many potential solvent developments) as well as possible step-changes, such as ion transfer membranes for oxygen production for integrated gasifier combined cycle and oxyfuel plants

  13. Welding Plutonium Storage Containers

    International Nuclear Information System (INIS)

    The outer can welder (OCW) in the FB-Line Facility at the Savannah River Site (SRS) is a Gas Tungsten Arc Weld (GTAW) system used to create outer canisters compliant with the Department of Energy 3013 Standard, DOE-STD-3013-2000, Stabilization, Packaging, and Storage of Plutonium-Bearing Materials. The key welding parameters controlled and monitored on the outer can welder Data Acquisition System (DAS) are weld amperage, weld voltage, and weld rotational speed. Inner 3013 canisters from the Bagless Transfer System that contain plutonium metal or plutonium oxide are placed inside an outer 3013 canister. The canister is back-filled with helium and welded using the outer can welder. The completed weld is screened to determine if it is satisfactory by reviewing the OCW DAS key welding parameters, performing a helium leak check, performing a visual examination by a qualified weld inspector, and performing digital radiography of the completed weld. Canisters with unsatisfactory welds are cut open and repackaged. Canisters with satisfactory welds are deemed compliant with the 3013 standard for long-term storage

  14. Optical information storage

    Energy Technology Data Exchange (ETDEWEB)

    Woike, T. [Koeln Univ., Inst. fuer Kristallography, Koeln (Germany)

    1996-11-01

    In order to increase storage capacity and data transfer velocity by about three orders of magnitude compared to CD or magnetic disc it is necessary to work with optical techniques, especially with holography. About 100 TByte can be stored in a waver of an area of 50 cm{sup 2} via holograms which corresponds to a density of 2.10{sup 9} Byte/mm{sup 2}. Every hologram contains data of 1 MByte, so that parallel-processing is possible for read-out. Using high-speed CCD-arrays a read-out velocity of 1 MByte/{mu}sec can be reached. Further, holographic technics are very important in solid state physics. We will discuss the existence of a space charge field in Sr{sub 1-x}Ba{sub x}Nb{sub 2}O{sub 6} doped with cerium and the physical properties of metastable states, which are suited for information storage. (author) 19 figs., 9 refs.

  15. Storage array reflection considerations

    International Nuclear Information System (INIS)

    The assumptions used for reflection conditions of single containers are fairly well established and consistently applied throughout the industry in nuclear criticality safety evaluations. Containers are usually considered to be either fully water reflected (i.e., surrounded by 6 to 12 in. of water) for safety calculations or reflected by 1 in. of water for nominal (structural material and air) conditions. Tables and figures are usually available for performing comparative evaluations of containers under various loading conditions. Reflection considerations used for evaluating the safety of storage arrays of fissile material are not as well established. When evaluating arrays, it has become more common for analysts to use calculations to demonstrate the safety of the array configuration. In performing these calculations, the analyst has considerable freedom concerning the assumptions made for modeling the reflection of the array. Considerations are given for the physical layout of the array with little or no discussion (or demonstration) of what conditions are bounded by the assumed reflection conditions. For example, an array may be generically evaluated by placing it in a corner of a room in which the opposing walls are far away. Typically, it is believed that complete flooding of the room is incredible, so the array is evaluated for various levels of water mist interspersed among array containers. This paper discusses some assumptions that are made regarding storage array reflection

  16. Spent fuel interim storage

    International Nuclear Information System (INIS)

    The official inauguration of the spent fuel interim storage took place on Monday July 28, 2003 at Cernavoda NNP. The inaugural event was attended by local and central public authority representatives, a Canadian Government delegation as well as newsmen from local and central mass media and numerous specialists from Cernavoda NPP compound. Mr Andrei Grigorescu, State Secretary with the Economy and Commerce Ministry, underlined in his talk the importance of this objective for the continuous development of nuclear power in Romania as well as for Romania's complying with the EU practice in this field. Also the excellent collaboration between the Canadian contractor AECL and the Romanian partners Nuclear Montaj, CITON, UTI, General Concret in the accomplishment of this unit at the planned terms and costs. On behalf of Canadian delegation, spoke Minister Don Boudria. He underlined the importance which the Canadian Government affords to the cooperation with Romania aiming at specific objectives in the field of nuclear power such as the Cernavoda NPP Unit 2 and spent fuel interim storage. After traditional cutting of the inaugural ribbon by the two Ministers the festivities continued on the Cernavoda NPP Compound with undersigning the documents regarding the project completion and a press conference

  17. Underground storage tank program

    International Nuclear Information System (INIS)

    Underground storage tanks, UST'S, have become a major component of the Louisville District's Environmental Support Program. The District's Geotechnical and Environmental Engineering Branch has spear-headed an innovative effort to streamline the time, effort and expense for removal, replacement, upgrade and associated cleanup of USTs at military and civil work installations. This program, called Yank-A-Tank, creates generic state-wide contracts for removal, remediation, installation and upgrade of storage tanks for which individual delivery orders are written under the basic contract. The idea is to create a ''JOC type'' contract containing all the components of work necessary to remove, reinstall or upgrade an underground or above ground tank. The contract documents contain a set of generic specifications and unit price books in addition to the standard ''boiler plate'' information. Each contract requires conformance to the specific regulations for the state in which it is issued. The contractor's bid consists of a bid factor which in the multiplier used with the prices in the unit price book. The solicitation is issued as a Request for Proposal (RPP) which allows the government to select a contractor based on technical qualification an well as bid factor. Once the basic contract is awarded individual delivery orders addressing specific areas of work are scoped, negotiated and awarded an modifications to the original contract. The delivery orders utilize the prepriced components and the contractor's factor to determine the value of the work

  18. Lysosomal lipid storage diseases.

    Science.gov (United States)

    Schulze, Heike; Sandhoff, Konrad

    2011-06-01

    Lysosomal lipid storage diseases, or lipidoses, are inherited metabolic disorders in which typically lipids accumulate in cells and tissues. Complex lipids, such as glycosphingolipids, are constitutively degraded within the endolysosomal system by soluble hydrolytic enzymes with the help of lipid binding proteins in a sequential manner. Because of a functionally impaired hydrolase or auxiliary protein, their lipid substrates cannot be degraded, accumulate in the lysosome, and slowly spread to other intracellular membranes. In Niemann-Pick type C disease, cholesterol transport is impaired and unesterified cholesterol accumulates in the late endosome. In most lysosomal lipid storage diseases, the accumulation of one or few lipids leads to the coprecipitation of other hydrophobic substances in the endolysosomal system, such as lipids and proteins, causing a "traffic jam." This can impair lysosomal function, such as delivery of nutrients through the endolysosomal system, leading to a state of cellular starvation. Therapeutic approaches are currently restricted to mild forms of diseases with significant residual catabolic activities and without brain involvement. PMID:21502308

  19. Magnetic energy storage

    International Nuclear Information System (INIS)

    The fusion program embraces low loss superconductor strand development with integration into cables capable of carrying 50 kA in pulsed mode at high fields. This evolvement has been paralleled with pulsed energy storage coil development and testing from tens of kJ at low fields to a 20 MJ prototype tokamak induction coil at 7.5 T. Energy transfer times have ranged from 0.7 ms to several seconds. Electric utility magnetic storage for prospective application is for diurnal load leveling with massive systems to store 10 GWh at 1.8 K in a dewar structure supported on bedrock underground. An immediate utility application is a 30 MJ system to be used to damp power oscillations on the Bonneville Power Administration electric transmission lines. An off-shoot of this last work is a new program for electric utility VAR control with the potential for use to suppress subsynchronous resonance. This paper presents work in progress, work planned, and recently completed unusual work

  20. A concept of an electricity storage system with 50 MWh storage capacity

    OpenAIRE

    Józef Paska; Mariusz K?os; Pawe? Antos; Grzegorz B?ajszczak

    2012-01-01

    Electricity storage devices can be divided into indirect storage technology devices (involving electricity conversion into another form of energy), and direct storage (in an electric or magnetic fi eld). Electricity storage technologies include: pumped-storage power plants, BES Battery Energy Storage, CAES Compressed Air Energy Storage, Supercapacitors, FES Flywheel Energy Storage, SMES Superconducting Magnetic Energy Storage, FC Fuel Cells reverse or operated in systems with electrolysers an...

  1. Storage and conservation of bagasse

    Energy Technology Data Exchange (ETDEWEB)

    Cusi, D.S.

    1980-08-01

    Storage of bagasse produced at harvest time becomes necessary when it is used for operations that are carried out continuously throughout the year, such as pulp and paper production. The sugar cane tissues suffer severe mechanical treatment in the sugar mills crushers, are further damaged in depithers and in many cases degraded while in storage. The processes of degradation are examined and handling and storage procedures are discussed which will minimize the quality and material losses.

  2. The underground nuclear wastes storage

    International Nuclear Information System (INIS)

    In the radioactive wastes management, the underground storage seems to be the long dated solution and the reference strategy. Then this storage has to be studied in term of accidental diffusion of radionuclides in the geologic site and in the food chain transfer. This document presents analytical models of diffusion which may help physicists to evaluate underground storage sites and the impacts on the environment and the human health. (A.L.B.)

  3. Storage Phosphors for Medical Imaging

    OpenAIRE

    Peter Willems; Dirk Vandenbroucke; Paul Leblans

    2011-01-01

    Computed radiography (CR) uses storage phosphor imaging plates for digital imaging. Absorbed X-ray energy is stored in crystal defects. In read-out the energy is set free as blue photons upon optical stimulation. In the 35 years of CR history, several storage phosphor families were investigated and developed. An explanation is given as to why some materials made it to the commercial stage, while others did not. The photo stimulated luminescence mechanism of the current commercial storage phos...

  4. IAEA spent fuel storage glossary

    International Nuclear Information System (INIS)

    The aim of this glossary is to provide a basis for improved international understanding of terms used in the important area of spent fuel storage technology. The glossary is the product of an IAEA Consultant Group with valuable input from a substantial list of reviewers. The glossary emphasizes fuel storage relevant to power reactors, but is also widely applicable to research reactors. The intention is to define terms from current technologies. Terms are limited to those directly related to spent fuel storage

  5. Article for thermal energy storage

    Science.gov (United States)

    Salyer, Ival O. (Dayton, OH)

    2000-06-27

    A thermal energy storage composition is provided which is in the form of a gel. The composition includes a phase change material and silica particles, where the phase change material may comprise a linear alkyl hydrocarbon, water/urea, or water. The thermal energy storage composition has a high thermal conductivity, high thermal energy storage, and may be used in a variety of applications such as in thermal shipping containers and gel packs.

  6. Chit-based Remote Storage

    OpenAIRE

    Paluska, Justin Mazzola; Stephen A. Ward

    2010-01-01

    We propose a model for reliable remote storage founded on contract law. Consumers submit their bits to storage providers in exchange for a chit. A chit is a cryptographically secure, verifiable contract between a consumer and the storage provider. A user can use the chit to request the bits from the provider. In return, the provider must be able to supply the chit's bits to the user according to the terms of the chit. If the storage provider cannot supply the bits, then the provider must pay ...

  7. The Petascale Data Storage Institute

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, Garth [Carnegie Mellon Univ., Pittsburgh, PA (United States); Long, Darrell [The Regents of the University of California, Santa Cruz, CA (United States); Honeyman, Peter [Univ. of Michigan, Ann Arbor, MI (United States); Grider, Gary [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kramer, William [National Energy Research Scientific Computing Center, Berkeley, CA (United States); Shalf, John [National Energy Research Scientific Computing Center, Berkeley, CA (United States); Roth, Philip [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Felix, Evan [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ward, Lee [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2013-07-01

    Petascale computing infrastructures for scientific discovery make petascale demands on information storage capacity, performance, concurrency, reliability, availability, and manageability.The Petascale Data Storage Institute focuses on the data storage problems found in petascale scientific computing environments, with special attention to community issues such as interoperability, community buy-in, and shared tools.The Petascale Data Storage Institute is a collaboration between researchers at Carnegie Mellon University, National Energy Research Scientific Computing Center, Pacific Northwest National Laboratory, Oak Ridge National Laboratory, Sandia National Laboratory, Los Alamos National Laboratory, University of Michigan, and the University of California at Santa Cruz.

  8. Improving wind power quality with energy storage

    DEFF Research Database (Denmark)

    Rasmussen, Claus Nygaard

    2009-01-01

    The results of simulation of the influence of energy storage on wind power quality are presented. Simulations are done using a mathematical model of energy storage. Results show the relation between storage power and energy, and the obtained increase in minimum available power from the combination of wind and storage. The introduction of storage enables smoothening of wind power on a timescale proportional to the storage energy. Storage does not provide availability of wind power at all times, b...

  9. Multi personal computer storage system. The solution of PACS storage

    International Nuclear Information System (INIS)

    Objective: According to the characteristics of digital medicine and demand of digitized management in hospital, the authors establish a storage system which is cheap, highly expansible, and reliable. Methods: The multi personal computer storage system (MPCSS) was constructed by assembling the hardware and software. The image data were archived from major server to storage PC by using NEUSOFT-PACS archiving manage system and backup on storage PC. The authors simulated the situation that the data on storage PC was lost and restored the data. The authors also expanded the storage system to enlarge its capacity. Results: Average transfer rate from MPCSS was 27.7 Mbit/s(1 byte = 8 bit); average cost for this system was 74 RMB/G; six cases in the 187 repeated reading of 100 patients failed. MPCSS can store backup and restore the image data, and can expand the storage size. Conclusion: MPCSS is very cheap compared with other high capacity systems or devices. It is feasible and suitable for digital image storage

  10. High Temperature Aquifer Storage

    Science.gov (United States)

    Ueckert, Martina; Niessner, Reinhard; Baumann, Thomas

    2015-04-01

    Combined heat and power generation (CHP) is highly efficient because excess heat is used for heating and/or process energy. However, the demand of heat energy varies considerably throughout the year while the demand for electrical energy is rather constant. It seems economically and ecologically highly beneficial for municipalities and large power consumers such as manufacturing plants to store excess heat in groundwater aquifers and to recuperate this energy at times of higher demand. Apart from the hydrogeological conditions, high transmissivity and favorable pressure gradients, the hydrochemical conditions are crucial for long-term operation. Within the project High Temperature Aquifer Storage, scientists investigate storage and recuperation of excess heat energy into the bavarian Malm aquifer. After one year of planning, construction, and the successful drilling of a research well to 495 m b.s.l. the first large scale heat storage test in the Malm aquifer was finished just before Christmas 2014. An enormous technical challenge was the disruption of the carbonate equilibrium - modeling results indicated a carbonate precipitation of 10-50 kg/d in the heat exchangers. The test included five injection pulses of hot water (60 °C up to 110 °C) and four tracer pulses, each consisting of a reactive and a conservative fluorescent dye. Injection and production rates were 15 L/s. About 4 TJ of heat energy were necessary to achieve the desired water temperatures. Electrical conductivity, pH and temperature were recorded at a bypass where also samples were taken. A laboratory container at the drilling site was equipped for the analysis of the concentration of the tracers and the cation concentrations at sampling intervals of down to 15 minutes. Additional water samples were taken and analyzed for major ions and trace elements in the laboratory. The disassembled heat exchanger proved that precipitation was successfully prevented by adding CO2 to the water before heating. Energy recovery during the first pulses was above 35 % and rising. As a side effect of the extremely good hydraulic conditions, the research well was flowing freely with 20 L/s which resulted in a significant mixing of the injected water with formation waters during production. The recovery rates for the tracers were above 60 % depending on the type of tracer.

  11. Spent fuel storage - dry storage options and issues

    International Nuclear Information System (INIS)

    The increase in the number of nuclear energy power generation facilities will require the ability to store the spent nuclear fuel for a long period until the host countries develop reprocessing or disposal options. Plants have storage pools which are closely associated with the operating units. These are excellent for short term storage, but require active maintenance and operations support which are not desirable for the long term. Over the past 25 years, dry storage options have been developed and implemented throughout the world. In recent years, protection against terrorist attack has become an increasing source of design objectives for these facilities, as well as the main nuclear plant. This paper explores the current design options of dry storage cask systems and examines some of the current design issues for above ground , in-ground, or below-ground storage of spent fuel in dry casks. (author)

  12. Reversible hydrogen storage materials

    Science.gov (United States)

    Ritter, James A. (Lexington, SC); Wang, Tao (Columbia, SC); Ebner, Armin D. (Lexington, SC); Holland, Charles E. (Cayce, SC)

    2012-04-10

    In accordance with the present disclosure, a process for synthesis of a complex hydride material for hydrogen storage is provided. The process includes mixing a borohydride with at least one additive agent and at least one catalyst and heating the mixture at a temperature of less than about 600.degree. C. and a pressure of H.sub.2 gas to form a complex hydride material. The complex hydride material comprises MAl.sub.xB.sub.yH.sub.z, wherein M is an alkali metal or group IIA metal, Al is the element aluminum, x is any number from 0 to 1, B is the element boron, y is a number from 0 to 13, and z is a number from 4 to 57 with the additive agent and catalyst still being present. The complex hydride material is capable of cyclic dehydrogenation and rehydrogenation and has a hydrogen capacity of at least about 4 weight percent.

  13. Ice storage system

    Energy Technology Data Exchange (ETDEWEB)

    Bartlett, T.A.; Froebe, R. [JCPenney Co. Inc., Dallas, TX (United States)

    1995-04-01

    The design and operation of JCPenney`s ice storage system takes conversion and/or addition of future controls and equipment into consideration. JCPenney began the conceptualization for its new Home Office in December 1988. After the analysis of a myriad of building styles, configurations, accommodations, services, and population, the construction documents phase began in March 1989 and was completed in October 1990. During this time period, the industry was in a quandary regarding the future of refrigerants and indoor air quality. This article will discuss the provisions that were made for unforeseen legislation; however, the concepts and efficiencies of the central plant and VAV systems were maintained with flexibility for conversion and/or addition of future controls and equipment.

  14. Spent fuel storage device

    International Nuclear Information System (INIS)

    Purpose: To obtain satisfactory countermeasure for system failures and fluctuations in fuel operation cycles. Constitution: A cooling unit comprising cooling plates of a quadrangle configuration and cooling pipes disposed therein in a zig-zag manner for directly cooling the pool water is disposed in a spent fuel storage pool. The inlet (or outlet) ends of the cooling pipe are respectively connected to the low temperature (or high temperature) sides of a cooling system for a ventilating auxiliary air conditioner cooling system respectively by way of pipeways, and automatic separation valves are interposed respectively to the pipeways. Upon failure of a fuel pool cooling and cleanup system, the automatic separation valve in the pipeway connected to the cooling system for the ventilating auxiliary air conditioner is opened and the cooling water cooled in the cooling system for instance, at 7 - 120C is circulated through the cooling plate to lower the temperature of the pool water. (Sekiya, K.)

  15. Superconducting energy storage

    Energy Technology Data Exchange (ETDEWEB)

    Giese, R.F.

    1993-10-01

    This report describes the status of energy storage involving superconductors and assesses what impact the recently discovered ceramic superconductors may have on the design of these devices. Our description is intended for R&D managers in government, electric utilities, firms, and national laboratories who wish an overview of what has been done and what remains to be done. It is assumed that the reader is acquainted with superconductivity, but not an expert on the topics discussed here. Indeed, it is the author`s aim to enable the reader to better understand the experts who may ask for the reader`s attention, support, or funding. This report may also inform scientists and engineers who, though expert in related areas, wish to have an introduction to our topic.

  16. Licensing of spent fuel dry storage and consolidated rod storage

    International Nuclear Information System (INIS)

    The results of this study, performed by Pacific Northwest Laboratory (PNL) and sponsored by the US Department of Energy (DOE), respond to the nuclear industry's recommendation that a report be prepared that collects and describes the licensing issues (and their resolutions) that confront a new applicant requesting approval from the US Nuclear Regulatory Commission (NRC) for dry storage of spent fuel or for large-scale storage of consolidated spent fuel rods in pools. The issues are identified in comments, questions, and requests from the NRC during its review of applicants' submittals. Included in the report are discussions of (1) the 18 topical reports on cask and module designs for dry storage fuel that have been submitted to the NRC, (2) the three license applications for dry storage of spent fuel at independent spent fuel storage installations (ISFSIs) that have been submitted to the NRC, and (3) the three applications (one of which was later withdrawn) for large-scale storage of consolidated fuel rods in existing spent fuel storage pools at reactors that were submitted tot he NRC. For each of the applications submitted, examples of some of the issues (and suggestions for their resolutions) are described. The issues and their resolutions are also covered in detail in an example in each of the three subject areas: (1) the application for the CASTOR V/21 dry spent fuel storage cask, (2) the application for the ISFSI for dry storage of spent fuel at Surry, and (3) the application for full-scale wet storage of consolidated spent fuel at Millstone-2. The conclusions in the report include examples of major issues that applicants have encountered. Recommendations for future applicants to follow are listed. 401 refs., 26 tabs

  17. Initial blood storage experiment

    Science.gov (United States)

    Surgenor, Douglas MACN.

    1988-01-01

    The design of the Initial Blood Storage Experiment (IBSE) was based upon a carefully controlled comparison between identical sets of human blood cell suspensions - red cells, white cell, and platelets - one set of which was transported aboard the Columbia on a 6 day 11 hour mission, and the other held on the ground. Both sets were carried inside stainless steel dewars within specially fabricated flight hardware. Individual bags of cell suspensions were randomly assigned with respect to ground vs orbit status, dewar chamber, and specific location within the dewar. To foster optimal preservation, each cell type was held under specific optimal conditions of pH, ionic strength, solute concentration, gas tension, and temperature. An added variable in this initial experiment was provided by the use of three different polymer/plasticizer formulations for the sealed bags which held the blood cells. At termination of the experiment, aliquots of the suspensions, identified only by code, were distributed to be assayed. Assays were selected to constitute a broad survey of cellular properties and thereby maximize the chances of detection of gravitational effects. A total of 74 different outcome measurements were reported for statistical analysis. When the measurements were completed, the results were entered into the IBSE data base, at which time the data were matched with the original blood bag numbers to determine their status with respect to polymer/plasticizer type, orbit status (orbit or ground), and storage position within the experimental hardware. The data were studied by analysis of variance. Initially, type of bag and orbital status were main factors; later more detailed analyses were made on specific issues such as position in the hardware and specific plastic. If the analysis of variance indicated a statistical significance at the 5 percent level the corresponding p-value was reported.

  18. A review of pumped energy storage schemes

    International Nuclear Information System (INIS)

    The comparative advantages and costs of pumped water storage, steam accumulators, storage of boiler feedwater , and compressed air storage in caverns are described. Boiler feedwater storage in caverns and pumped water storage are most economical. All systems are costly enough to justify developing reactors with load following capabilities. (E.C.B.)

  19. Study of mined storage caverns

    International Nuclear Information System (INIS)

    All available information concerning 75 mined storage caverns as well as the investigative techniques utilized to determine the feasibility of a cavern site is presented. Potential new storage caverns may be for products such as high or low vapor pressure petroleum products, other chemical products, aid industrial or nuclear waste

  20. Electrochemistry and Storage Panel Report

    Science.gov (United States)

    Stedman, J. K.; Halpert, G.

    1984-01-01

    Design and performance requirements for electrochemical power storage systems are discussed and some of the approaches towards satisfying these constraints are described. Geosynchronous and low Earth orbit applications, radar type load constraints, and high voltage systems requirements are addressed. In addition, flywheel energy storage is discussed.

  1. Nanostructured materials for hydrogen storage

    Science.gov (United States)

    Williamson, Andrew J. (Pleasanton, CA); Reboredo, Fernando A. (Pleasanton, CA)

    2007-12-04

    A system for hydrogen storage comprising a porous nano-structured material with hydrogen absorbed on the surfaces of the porous nano-structured material. The system of hydrogen storage comprises absorbing hydrogen on the surfaces of a porous nano-structured semiconductor material.

  2. Type I Glycogen Storage Disease

    Science.gov (United States)

    ... and kidneys, and possibly a genetic test or liver biopsy. How is type I glycogen storage disease treated? ... child’s dietary needs and restrictions. Who is at risk for type I glycogen storage disease? GSD I is an inherited ... Copyright 2015 American Liver Foundation. All Rights Reserved. The American Liver Foundation ...

  3. SEED ARCHIVE AND STORAGE FACILITIES

    Science.gov (United States)

    Sugar beet seed storage facilities were updated, and seed is stored at 4C and 25% relative humidity. Over 15,000 packages of seed have been moved into the new storage facility. We used results from field scale germination studies to discard over 3,500 seedlots that showed no emergence. Current seed ...

  4. Hydrogen storage in capillary arrays

    International Nuclear Information System (INIS)

    We have developed the technology of hydrogen storage in capillary arrays, and we present the corresponding theoretical background. The technology can be effectively used for safe transportation and storage of highly pressurized hydrogen in mobile systems, ranging from domestic electronic devices to ground and sea vehicles

  5. SOURCE ASSESSMENT: COAL STORAGE PILES

    Science.gov (United States)

    This report describes a study of atmospheric emissions from coal storage piles. Fugitive emissions of dust and gases are emitted from coal storage piles. The average emission factor for respirable particulate (< 7 micrometer) is 6.4 mg/kg per annum; this factor describes the emis...

  6. Hydrogen storage: beyond conventional methods.

    Science.gov (United States)

    Dalebrook, Andrew F; Gan, Weijia; Grasemann, Martin; Moret, Séverine; Laurenczy, Gábor

    2013-10-01

    The efficient storage of hydrogen is one of three major hurdles towards a potential hydrogen economy. This report begins with conventional storage methods for hydrogen and broadly covers new technology, ranging from physical media involving solid adsorbents, to chemical materials including metal hydrides, ammonia borane and liquid precursors such as alcohols and formic acid. PMID:23964360

  7. Different types of intermediate storages

    International Nuclear Information System (INIS)

    Spent nuclear fuel elements require a period of ''cooling'' after withdrawal from a nuclear reactor. This is done in water storage pools located at the reactor facility. The minimum storage time prior to subsequent operations with the spent fuel is recognized as six to twelve months. After the initial storage period for cooling, the subsequent storage time in water pools could vary from a few years to several decades. Up to 1980, about 15.700 MTHM of spent LWR and HWR fuel have been discharged from nuclear stations. Since only a small fraction thereof has been already reprocessed. The spent fuel assemblies are stored at the reactor-side (AR) in reactor basins in normal or compact racks and away from reactor (AFR) in water pool storages awaiting either reprocessing or final disposal depending on the fuel cycle concept chosen by the individual countries. (orig.)

  8. Anthology of dry storage solutions

    International Nuclear Information System (INIS)

    Around 35,000 PWR, BWR or Veer used fuel elements with various enrichment value up to 5%, various cooling time down to 2 years and various burn-ups up to 60,000 Mwd/tU are currently stored in AREVA dry storage solutions. These solutions are delivered in the United States, in Japan and in many European countries like Belgium, Switzerland, Italy, Armenia and Germany. With more than 1000 dry storage solutions delivered all over the world AREVA is the leader on this market. Dealing with dry storage is not an easy task. Products have to be flexible, to be adapted to customer needs and to the national regulations which may stipulate very strict tests such as airplane crash or simulation of earthquake. To develop a dry storage solution for a foreign country means to deal with its national competent authorities. All the national competent authorities do not have the same requirements. Storage conditions may also be different

  9. Anthology of dry storage solutions

    Energy Technology Data Exchange (ETDEWEB)

    Allimann, Nathalie; Otton, Camille [AREVA, Paris (France)

    2012-03-15

    Around 35,000 PWR, BWR or Veer used fuel elements with various enrichment value up to 5%, various cooling time down to 2 years and various burn-ups up to 60,000 Mwd/tU are currently stored in AREVA dry storage solutions. These solutions are delivered in the United States, in Japan and in many European countries like Belgium, Switzerland, Italy, Armenia and Germany. With more than 1000 dry storage solutions delivered all over the world AREVA is the leader on this market. Dealing with dry storage is not an easy task. Products have to be flexible, to be adapted to customer needs and to the national regulations which may stipulate very strict tests such as airplane crash or simulation of earthquake. To develop a dry storage solution for a foreign country means to deal with its national competent authorities. All the national competent authorities do not have the same requirements. Storage conditions may also be different.

  10. Storage unit for metal hydrides

    International Nuclear Information System (INIS)

    It is well known that the CANDU heavy water reactors produce tritium in the moderator and coolant circuits as a result of neutron capture by the deuterium nuclei. The Cernavoda Nuclear Power Plant is a CANDU type reactor and we could expect in the future a contamination of the surrounding area as a result of the increase of the quantity of tritium. Therefore, in order to maintain a minimum level of radioactivity in the area, tritium, a low level waste product of nuclear fission, must be continuously removed from the moderator heat transport systems. Tritium is a beta emitter and decays to 3 He emitting a beta particle and a neutrino. The energy of the beta particles vary from 0 to 18.6 keV with an average energy of 5.69 keV. There are few major ways used in the storage of the hydrogen isotopes, namely: gaseous storage, liquid hydrogen storage and metal hydride storage. Metal hydrides offer safe and efficient a way for tritium processing, including storage, pumping, compression, transport and purification. Tritium removed from the tritiated heavy water requests its storage in special vessels able to ensure protection and safety of environment and personal. This paper presents our achievements in designing a storage vessel for hydrogen isotope processing. The vessel for tritium storage as metal tritides is an austenitic stainless steel container designed to accommodate also the helium generated by the complete decay of tritium. It is equipped with valves allowing circulation of the gas during the hydriding process. Due to the hydriding and de-hydriding cycles, fine particles of the metal tritide could be produced. In order to prevent their deposition on the valves, the entry and exit ways of the vessel are protected by sintered steel filters. The metal selected for tritium storage is the depleted uranium. Using 500 g uranium, the storage capacity of the vessel is maximum 3 moles of hydrogen isotopes. (author)

  11. Electrochemical hydrogen Storage Systems

    International Nuclear Information System (INIS)

    As the global need for energy increases, scientists and engineers have found a possible solution by using hydrogen to power our world. Although hydrogen can be combusted as a fuel, it is considered an energy carrier for use in fuel cells wherein it is consumed (oxidized) without the production of greenhouse gases and produces electrical energy with high efficiency. Chemical storage of hydrogen involves release of hydrogen in a controlled manner from materials in which the hydrogen is covalently bound. Sodium borohydride and aminoborane are two materials given consideration as chemical hydrogen storage materials by the US Department of Energy. A very significant barrier to adoption of these materials as hydrogen carriers is their regeneration from 'spent fuel,' i.e., the material remaining after discharge of hydrogen. The U.S. Department of Energy (DOE) formed a Center of Excellence for Chemical Hydrogen Storage, and this work stems from that project. The DOE has identified boron hydrides as being the main compounds of interest as hydrogen storage materials. The various boron hydrides are then oxidized to release their hydrogen, thereby forming a 'spent fuel' in the form of a lower boron hydride or even a boron oxide. The ultimate goal of this project is to take the oxidized boron hydrides as the spent fuel and hydrogenate them back to their original form so they can be used again as a fuel. Thus this research is essentially a boron hydride recycling project. In this report, research directed at regeneration of sodium borohydride and aminoborane is described. For sodium borohydride, electrochemical reduction of boric acid and sodium metaborate (representing spent fuel) in alkaline, aqueous solution has been investigated. Similarly to literature reports (primarily patents), a variety of cathode materials were tried in these experiments. Additionally, approaches directed at overcoming electrostatic repulsion of borate anion from the cathode, not described in the previous literature for electrochemical reduction of spent fuels, have been attempted. A quantitative analytical method for measuring the concentration of sodium borohydride in alkaline aqueous solution has been developed as part of this work and is described herein. Finally, findings from stability tests for sodium borohydride in aqueous solutions of several different compositions are reported. For aminoborane, other research institutes have developed regeneration schemes involving tributyltin hydride. In this report, electrochemical reduction experiments attempting to regenerate tributyltin hydride from tributyltin chloride (a representative by-product of the regeneration scheme) are described. These experiments were performed in the non-aqueous solvents acetonitrile and 1,2-dimethoxyethane. A non-aqueous reference electrode for electrolysis experiments in acetonitrile was developed and is described. One class of boron hydrides, called polyhedral boranes, became of interest to the DOE due to their ability to contain a sufficient amount of hydrogen to meet program goals and because of their physical and chemical safety attributes. Unfortunately, the research performed here has shown that polyhedral boranes do not react in such a way as to allow enough hydrogen to be released, nor do they appear to undergo hydrogenation from the spent fuel form back to the original hydride. After the polyhedral boranes were investigated, the project goals remained the same but the hydrogen storage material was switched by the DOE to ammonia borane. Ammonia borane was found to undergo an irreversible hydrogen release process, so a direct hydrogenation was not able to occur. To achieve the hydrogenation of the spent ammonia borane fuel, an indirect hydrogenation reaction is possible by using compounds called organotin hydrides. In this process, the organotin hydrides will hydrogenate the spent ammonia borane fuel at the cost of their own oxidation, which forms organotin halides. To enable a closed-loop cycle, our task was then to be able to hydrogenate the organotin halides back to their hydride form. In addition to this experimental work, a parallel project was carried out to develop a new model of electrochemical impedance spectroscopy (EIS) that could be used to define the mechanisms of the electrochemical hydrogenation reactions. The EIS technique is capable of probing complex chemical and electrochemical reactions, and our model was written into a computer code that allowed the input of experimental EIS data and the extraction of kinetic parameters based on a best-fit analysis of theoretical reaction schemes. Finally, electrochemical methods for hydrogenating organic and metallo-organic materials have been explored.

  12. Electrochemical hydrogen Storage Systems

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Digby Macdonald

    2010-08-09

    As the global need for energy increases, scientists and engineers have found a possible solution by using hydrogen to power our world. Although hydrogen can be combusted as a fuel, it is considered an energy carrier for use in fuel cells wherein it is consumed (oxidized) without the production of greenhouse gases and produces electrical energy with high efficiency. Chemical storage of hydrogen involves release of hydrogen in a controlled manner from materials in which the hydrogen is covalently bound. Sodium borohydride and aminoborane are two materials given consideration as chemical hydrogen storage materials by the US Department of Energy. A very significant barrier to adoption of these materials as hydrogen carriers is their regeneration from 'spent fuel,' i.e., the material remaining after discharge of hydrogen. The U.S. Department of Energy (DOE) formed a Center of Excellence for Chemical Hydrogen Storage, and this work stems from that project. The DOE has identified boron hydrides as being the main compounds of interest as hydrogen storage materials. The various boron hydrides are then oxidized to release their hydrogen, thereby forming a 'spent fuel' in the form of a lower boron hydride or even a boron oxide. The ultimate goal of this project is to take the oxidized boron hydrides as the spent fuel and hydrogenate them back to their original form so they can be used again as a fuel. Thus this research is essentially a boron hydride recycling project. In this report, research directed at regeneration of sodium borohydride and aminoborane is described. For sodium borohydride, electrochemical reduction of boric acid and sodium metaborate (representing spent fuel) in alkaline, aqueous solution has been investigated. Similarly to literature reports (primarily patents), a variety of cathode materials were tried in these experiments. Additionally, approaches directed at overcoming electrostatic repulsion of borate anion from the cathode, not described in the previous literature for electrochemical reduction of spent fuels, have been attempted. A quantitative analytical method for measuring the concentration of sodium borohydride in alkaline aqueous solution has been developed as part of this work and is described herein. Finally, findings from stability tests for sodium borohydride in aqueous solutions of several different compositions are reported. For aminoborane, other research institutes have developed regeneration schemes involving tributyltin hydride. In this report, electrochemical reduction experiments attempting to regenerate tributyltin hydride from tributyltin chloride (a representative by-product of the regeneration scheme) are described. These experiments were performed in the non-aqueous solvents acetonitrile and 1,2-dimethoxyethane. A non-aqueous reference electrode for electrolysis experiments in acetonitrile was developed and is described. One class of boron hydrides, called polyhedral boranes, became of interest to the DOE due to their ability to contain a sufficient amount of hydrogen to meet program goals and because of their physical and chemical safety attributes. Unfortunately, the research performed here has shown that polyhedral boranes do not react in such a way as to allow enough hydrogen to be released, nor do they appear to undergo hydrogenation from the spent fuel form back to the original hydride. After the polyhedral boranes were investigated, the project goals remained the same but the hydrogen storage material was switched by the DOE to ammonia borane. Ammonia borane was found to undergo an irreversible hydrogen release process, so a direct hydrogenation was not able to occur. To achieve the hydrogenation of the spent ammonia borane fuel, an indirect hydrogenation reaction is possible by using compounds called organotin hydrides. In this process, the organotin hydrides will hydrogenate the spent ammonia borane fuel at the cost of their own oxidation, which forms organotin halides. To enable a closed-loop cycle, our task was then to be able to hydrogenate the organotin halides back to their hydride form. In addition to this experimental work, a parallel project was carried out to develop a new model of electrochemical impedance spectroscopy (EIS) that could be used to define the mechanisms of the electrochemical hydrogenation reactions. The EIS technique is capable of probing complex chemical and electrochemical reactions, and our model was written into a computer code that allowed the input of experimental EIS data and the extraction of kinetic parameters based on a best-fit analysis of theoretical reaction schemes. Finally, electrochemical methods for hydrogenating organic and metallo-organic materials have been explored.

  13. Hydrogen storage methods.

    Science.gov (United States)

    Züttel, Andreas

    2004-04-01

    Hydrogen exhibits the highest heating value per mass of all chemical fuels. Furthermore, hydrogen is regenerative and environmentally friendly. There are two reasons why hydrogen is not the major fuel of today's energy consumption. First of all, hydrogen is just an energy carrier. And, although it is the most abundant element in the universe, it has to be produced, since on earth it only occurs in the form of water and hydrocarbons. This implies that we have to pay for the energy, which results in a difficult economic dilemma because ever since the industrial revolution we have become used to consuming energy for free. The second difficulty with hydrogen as an energy carrier is its low critical temperature of 33 K (i.e. hydrogen is a gas at ambient temperature). For mobile and in many cases also for stationary applications the volumetric and gravimetric density of hydrogen in a storage material is crucial. Hydrogen can be stored using six different methods and phenomena: (1) high-pressure gas cylinders (up to 800 bar), (2) liquid hydrogen in cryogenic tanks (at 21 K), (3) adsorbed hydrogen on materials with a large specific surface area (at Ttemperature), (5) chemically bonded in covalent and ionic compounds (at ambient pressure), or (6) through oxidation of reactive metals, e.g. Li, Na, Mg, Al, Zn with water. The most common storage systems are high-pressure gas cylinders with a maximum pressure of 20 MPa (200 bar). New lightweight composite cylinders have been developed which are able to withstand pressures up to 80 MPa (800 bar) and therefore the hydrogen gas can reach a volumetric density of 36 kg.m(-3), approximately half as much as in its liquid state. Liquid hydrogen is stored in cryogenic tanks at 21.2 K and ambient pressure. Due to the low critical temperature of hydrogen (33 K), liquid hydrogen can only be stored in open systems. The volumetric density of liquid hydrogen is 70.8 kg.m(-3), and large volumes, where the thermal losses are small, can cause hydrogen to reach a system mass ratio close to one. The highest volumetric densities of hydrogen are found in metal hydrides. Many metals and alloys are capable of reversibly absorbing large amounts of hydrogen. Charging can be done using molecular hydrogen gas or hydrogen atoms from an electrolyte. The group one, two and three light metals (e.g. Li, Mg, B, Al) can combine with hydrogen to form a large variety of metal-hydrogen complexes. These are especially interesting because of their light weight and because of the number of hydrogen atoms per metal atom, which is two in many cases. Hydrogen can also be stored indirectly in reactive metals such as Li, Na, Al or Zn. These metals easily react with water to the corresponding hydroxide and liberate the hydrogen from the water. Since water is the product of the combustion of hydrogen with either oxygen or air, it can be recycled in a closed loop and react with the metal. Finally, the metal hydroxides can be thermally reduced to metals in a solar furnace. This paper reviews the various storage methods for hydrogen and highlights their potential for improvement and their physical limitations. PMID:15085273

  14. On-line storage versus local storage for mobile users

    OpenAIRE

    Huang, Liang

    2006-01-01

    When a user has a mobile device with lots of built-in functions, what would they like to do with it? Of course, interactive voice and videoconferencing, sending SMS & Instant Messaging, listening to music, taking photos, etc. People want to have a device with a large storage capacity, much as they do on a desktop or laptop PC. But sometimes the user does not have sufficient local storage capacity on their mobile device. Online storage is a good solution for this, but the limited battery c...

  15. Carbon Capture and Storage

    Energy Technology Data Exchange (ETDEWEB)

    Friedmann, S

    2007-10-03

    Carbon capture and sequestration (CCS) is the long-term isolation of carbon dioxide from the atmosphere through physical, chemical, biological, or engineered processes. This includes a range of approaches including soil carbon sequestration (e.g., through no-till farming), terrestrial biomass sequestration (e.g., through planting forests), direct ocean injection of CO{sub 2} either onto the deep seafloor or into the intermediate depths, injection into deep geological formations, or even direct conversion of CO{sub 2} to carbonate minerals. Some of these approaches are considered geoengineering (see the appropriate chapter herein). All are considered in the 2005 special report by the Intergovernmental Panel on Climate Change (IPCC 2005). Of the range of options available, geological carbon sequestration (GCS) appears to be the most actionable and economic option for major greenhouse gas reduction in the next 10-30 years. The basis for this interest includes several factors: (1) The potential capacities are large based on initial estimates. Formal estimates for global storage potential vary substantially, but are likely to be between 800 and 3300 Gt of C (3000 and 10,000 Gt of CO{sub 2}), with significant capacity located reasonably near large point sources of the CO{sub 2}. (2) GCS can begin operations with demonstrated technology. Carbon dioxide has been separated from large point sources for nearly 100 years, and has been injected underground for over 30 years (below). (3) Testing of GCS at intermediate scale is feasible. In the US, Canada, and many industrial countries, large CO{sub 2} sources like power plants and refineries lie near prospective storage sites. These plants could be retrofit today and injection begun (while bearing in mind scientific uncertainties and unknowns). Indeed, some have, and three projects described here provide a great deal of information on the operational needs and field implementation of CCS. Part of this interest comes from several key documents written in the last three years that provide information on the status, economics, technology, and impact of CCS. These are cited throughout this text and identified as key references at the end of this manuscript. When coupled with improvements in energy efficiency, renewable energy supplies, and nuclear power, CCS help dramatically reduce current and future emissions (US CCTP 2005, MIT 2007). If CCS is not available as a carbon management option, it will be much more difficult and much more expensive to stabilize atmospheric CO{sub 2} emissions. Recent estimates put the cost of carbon abatement without CCS to be 30-80% higher that if CCS were to be available (Edmonds et al. 2004).

  16. Update and overview of storage technologies

    International Nuclear Information System (INIS)

    This presentation includes a brief overview of on-site spent fuel storage technologies and an update of developments in on-site storage projects since the EEI/UWASTE Spent Fuel Storage Handbook was published in December 1990

  17. Global Carbon Dioxide Storage Potential and Costs

    International Nuclear Information System (INIS)

    An estimate is given of the worldwide CO2 storage potential. The potential is given by region and for five types of underground storage reservoirs. The report also presents an estimate of costs for capture and storage of carbon dioxide

  18. Lithium ion storage between graphenes

    Directory of Open Access Journals (Sweden)

    Chan Yue

    2011-01-01

    Full Text Available Abstract In this article, we investigate the storage of lithium ions between two parallel graphene sheets using the continuous approximation and the 6-12 Lennard-Jones potential. The continuous approximation assumes that the carbon atoms can be replaced by a uniform distribution across the surface of the graphene sheets so that the total interaction potential can be approximated by performing surface integrations. The number of ion layers determines the major storage characteristics of the battery, and our results show three distinct ionic configurations, namely single, double, and triple ion forming layers between graphenes. The number densities of lithium ions between the two graphenes are estimated from existing semi-empirical molecular orbital calculations, and the graphene sheets giving rise to the triple ion layers admit the largest storage capacity at all temperatures, followed by a marginal decrease of storage capacity for the case of double ion layers. These two configurations exceed the maximum theoretical storage capacity of graphite. Further, on taking into account the charge-discharge property, the double ion layers are the most preferable choice for enhanced lithium storage. Although the single ion layer provides the least charge storage, it turns out to be the most stable configuration at all temperatures. One application of the present study is for the design of future high energy density alkali batteries using graphene sheets as anodes for which an analytical formulation might greatly facilitate rapid computational results.

  19. Hydrogen storage in nanotubes & nanostructures

    Directory of Open Access Journals (Sweden)

    George E. Froudakis

    2011-07-01

    Full Text Available Over the last several years, a significant share of the scientific community has focused its attention on the hydrogen storage problem. Since 1997, when carbon nanotubes appeared to be a promising storage material, many theoretical and experimental groups have investigated the hydrogen storage capacity of these carbon nanostructures. These efforts were not always successful and consequently, the results obtained were often controversial. In the current review we attempt to summarize some the highlights of the work on hydrogen storage in various types of nanotube and nanostructure, in a critical way. The nature of the interaction between hydrogen and the host nanomaterials, as revealed through theoretical modeling, helps us understand the basic mechanisms of hydrogen storage. Analysis of the results reveals why high hydrogen storage capacity at ambient conditions, which meets the DOE targets, cannot occur in bare carbon nanotubes. Through our analysis we also propose guidelines to enhance the hydrogen storage capacity of already synthesized materials and recommend advanced materials for this application.

  20. Interim and final storage casks

    International Nuclear Information System (INIS)

    The disposal of radioactive waste is a huge social challenge in Germany and all over the world. As is well known the search for a site for a final repository for high-level waste in Germany is not complete. Therefore, interim storage facilities for radioactive waste were built at plant sites in Germany. The waste is stored in these storage facilities in appropriate storage and transport casks until the transport in a final repository can be carried out. Licensing of the storage and transport casks aimed for use in the public space is done according to the traffic laws and for handling in the storage facility according to nuclear law. Taking into account the activity of the waste to be stored, different containers are in use, so that experience is available from the licensing and operation in interim storage facilities. The large volume of radioactive waste to be disposed of after the shut-down of power generation in nuclear power stations makes it necessary for large quantities of licensed storage and transport casks to be provided soon.

  1. Radioactive material dry storage facility

    International Nuclear Information System (INIS)

    In a storage chamber for storing tubes for densely containing radioactive materials, the containing tubes are supported by a floor wall and a ceiling wall of the storage chamber, and the storage chamber is connected with a sucking portion for taking air from the outside to an upstream portion and with an air exhaustion portion for releasing the air warmed by heat exchange with the containing tubes to a downstream portion. A bypass flow channel is disposed to the circumferential walls for introducing a portion of external air, and a blowing port is disposed to the bypass flow channel, so that the bypass stream is blown out into the storage chamber. In addition, the diameter of the blowing port of the bypass flow channel is made greater toward backward of the storage chamber. A resistance body is disposed to an upstream portion on the side wall of the storage chamber. In addition, a deflection plate supported by the ceiling wall and the floor wall is disposed in the vicinity of the side wall of the storage chamber. The temperature distribution of the containing tubes and the floor wall/ceiling wall can be made uniform thereby enabling to mitigate thermal stresses. (N.H.)

  2. Nuclear materials management storage study

    International Nuclear Information System (INIS)

    The Office of Weapons and Materials Planning (DP-27) requested the Planning Support Group (PSG) at the Savannah River Site to help coordinate a Departmental complex-wide nuclear materials storage study. This study will support the development of management strategies and plans until Defense Programs' Complex 21 is operational by DOE organizations that have direct interest/concerns about or responsibilities for nuclear material storage. They include the Materials Planning Division (DP-273) of DP-27, the Office of the Deputy Assistant Secretary for Facilities (DP-60), the Office of Weapons Complex Reconfiguration (DP-40), and other program areas, including Environmental Restoration and Waste Management (EM). To facilitate data collection, a questionnaire was developed and issued to nuclear materials custodian sites soliciting information on nuclear materials characteristics, storage plans, issues, etc. Sites were asked to functionally group materials identified in DOE Order 5660.1A (Management of Nuclear Materials) based on common physical and chemical characteristics and common material management strategies and to relate these groupings to Nuclear Materials Management Safeguards and Security (NMMSS) records. A database was constructed using 843 storage records from 70 responding sites. The database and an initial report summarizing storage issues were issued to participating Field Offices and DP-27 for comment. This report presents the background for the Storage Study and an initial, unclassified summary of storage issues and concerns identified by the sites

  3. Opportunities in independent gas storage

    International Nuclear Information System (INIS)

    The range of business opportunities currently available for the midstream oil and gas business were discussed with particular focus on storage opportunities. Alberta Energy Co. (AEC) Ltd.'s two midstream business units include AEC Pipelines and Gas Processing, and AEC Storage and Hub Services. These two businesses provide the company with good investment returns, stable cash flow, and some significant strategic synergies with their exploration and production businesses. In 1988, the AECO C Hub in southeastern Alberta was created as an outgrowth of AEC's gas production operations on the Suffield block, where they had depleted gas reservoirs with high rock quality suitable for high deliverability storage. With the AECO C Hub, AEC was able to offer firm storage contracts of from 1 to 20 years, and to introduce short term interruptible parking and lending services, title exchange, a spot price index for greater price discovery, and an electronic nomination system. AEC is currently completing construction of their second commercial storage facility, the Wild Goose project, in northern California. Debottlenecking' the Western Canada supply basin should provide additional opportunities for further expansion not only for AEC but also for other midstream service providers. Opportunities are especially available in the areas of new storage facilities to serve location-specific needs, replacement of declining storage capacity, replacement of retiring facilities, technological optimization of existing facilities, more flexible, higher deliverability facilities and commercial optimization of existing facilities. A map of the hubs and market centres of North America are included. 5 figs

  4. Solar Thermal Storage System

    Directory of Open Access Journals (Sweden)

    Arjun A. Abhyankar

    2012-06-01

    Full Text Available Increasing energy consumption, shrinking resources and rising energy costs will have significant impact on our standard of living for future generations. In this situation, the development of alternative, cost effective sources of energy has to be a priority. This project presents the advanced technology and some of the unique features of a novel solar system that utilizes solar energy for space heating and water heating purpose in residential housing and commercial buildings. The improvements in solar technology offers a significant cost reduction, to a level where the solar system can compete with the energy costs from existing sources. The main goal of the project is to investigate new or advanced solutions for storing heat in systems providing heating. which can be achieved using phase change material(PCM.A phase change material with a melting/solidification temperature of 50ºC to 60ºC is used for solar heat storage. When the PCM undergoes the phase change, it can absorb or release a large amount of energy as latent heat. This heat can be used for further applications like water heating and space heating purposes. Thus solar thermal energy is widely use

  5. Liquid metal storage tank

    International Nuclear Information System (INIS)

    The present invention concerns a liquid metal storage tank used for an FBR type reactor plant. It comprises a tank main body disposed in a pit chamber, a sealing tub disposed at an upper outer circumferential surface of the tank main body, a roof portion which closes the opening a the upper end of the pit chamber, a sealing partitioning cylinder suspended from the lower surface of the roof and having its lower end extended to the inside of the tub and a sealing liquid metal filled in the tub. The tank main body is kept at a high temperature by the liquid metal while the roof in the upper portion of the pit chamber is kept at a low temperature. Further, since the tank main body and the inside of the pit chamber are sealed by the sealing partitioning cylinder, no large thermal stresses are caused to the wall of the tank main body. Even if hydrogen gases are generated in the tank main body, since they can be released to the inside of the pit chamber, the integrity of the tank can be maintained, even if abrupt pressure elevation is caused in the tank main body. (I.S.)

  6. Conductive lithium storage electrode

    Energy Technology Data Exchange (ETDEWEB)

    Chiang, Yet-Ming; Chung, Sung-Yoon; Bloking, Jason T; Andersson, Anna M

    2014-10-07

    A compound comprising a composition A.sub.x(M'.sub.1-aM''.sub.a).sub.y(XD.sub.4).sub.z, A.sub.x(M'.sub.1-aM''.sub.a).sub.y(DXD.sub.4).sub.z, or A.sub.x(M'.sub.1-aM''.sub.a).sub.y(X.sub.2D.sub.7).sub.z, (A.sub.1-aM''.sub.a).sub.xM'.sub.y(XD.sub.4).sub.z, (A.sub.1-aM''.sub.a).sub.xM'.sub.y(DXD.sub.4).sub.z, or (A.sub.1-aM''.sub.a).sub.xM'.sub.y(X.sub.2D.sub.7).sub.z. In the compound, A is at least one of an alkali metal and hydrogen, M' is a first-row transition metal, X is at least one of phosphorus, sulfur, arsenic, molybdenum, and tungsten, M'' any of a Group IIA, IIIA, IVA, VA, VIA, VIIA, VIIIA, IB, IIB, IIIB, IVB, VB, and VIB metal, D is at least one of oxygen, nitrogen, carbon, or a halogen, 0.0001storage batteries.

  7. Disk storage at CERN

    CERN Document Server

    Mascetti, L; Chan, B; Espinal, X; Fiorot, A; Labrador, H Gonz; Iven, J; Lamanna, M; Presti, G Lo; Mo?cicki, JT; Peters, AJ; Ponce, S; Rousseau, H; van der Ster, D

    2015-01-01

    CERN IT DSS operates the main storage resources for data taking and physics analysis mainly via three system: AFS, CASTOR and EOS. The total usable space available on disk for users is about 100 PB (with relative ratios 1:20:120). EOS actively uses the two CERN Tier0 centres (Meyrin and Wigner) with 50:50 ratio. IT DSS also provide sizeable on-demand resources for IT services most notably OpenStack and NFS-based clients: this is provided by a Ceph infrastructure (3 PB) and few proprietary servers (NetApp). We will describe our operational experience and recent changes to these systems with special emphasis to the present usages for LHC data taking, the convergence to commodity hardware (nodes with 200-TB each with optional SSD) shared across all services. We also describe our experience in coupling commodity and home-grown solution (e.g. CERNBox integration in EOS, Ceph disk pools for AFS, CASTOR and NFS) and finally the future evolution of these systems for WLCG and beyond.

  8. Disk storage at CERN

    Science.gov (United States)

    Mascetti, L.; Cano, E.; Chan, B.; Espinal, X.; Fiorot, A.; González Labrador, H.; Iven, J.; Lamanna, M.; Lo Presti, G.; Mo?cicki, JT; Peters, AJ; Ponce, S.; Rousseau, H.; van der Ster, D.

    2015-12-01

    CERN IT DSS operates the main storage resources for data taking and physics analysis mainly via three system: AFS, CASTOR and EOS. The total usable space available on disk for users is about 100 PB (with relative ratios 1:20:120). EOS actively uses the two CERN Tier0 centres (Meyrin and Wigner) with 50:50 ratio. IT DSS also provide sizeable on-demand resources for IT services most notably OpenStack and NFS-based clients: this is provided by a Ceph infrastructure (3 PB) and few proprietary servers (NetApp). We will describe our operational experience and recent changes to these systems with special emphasis to the present usages for LHC data taking, the convergence to commodity hardware (nodes with 200-TB each with optional SSD) shared across all services. We also describe our experience in coupling commodity and home-grown solution (e.g. CERNBox integration in EOS, Ceph disk pools for AFS, CASTOR and NFS) and finally the future evolution of these systems for WLCG and beyond.

  9. ICI optical data storage tape: An archival mass storage media

    Science.gov (United States)

    Ruddick, Andrew J.

    1993-01-01

    At the 1991 Conference on Mass Storage Systems and Technologies, ICI Imagedata presented a paper which introduced ICI Optical Data Storage Tape. This paper placed specific emphasis on the media characteristics and initial data was presented which illustrated the archival stability of the media. More exhaustive analysis that was carried out on the chemical stability of the media is covered. Equally important, it also addresses archive management issues associated with, for example, the benefits of reduced rewind requirements to accommodate tape relaxation effects that result from careful tribology control in ICI Optical Tape media. ICI Optical Tape media was designed to meet the most demanding requirements of archival mass storage. It is envisaged that the volumetric data capacity, long term stability and low maintenance characteristics demonstrated will have major benefits in increasing reliability and reducing the costs associated with archival storage of large data volumes.

  10. ADVANCED UNDERGROUND GAS STORAGE CONCEPTS REFRIGERATED-MINED CAVERN STORAGE

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    Limited demand and high cost has prevented the construction of hard rock caverns in this country for a number of years. The storage of natural gas in mined caverns may prove technically feasible if the geology of the targeted market area is suitable; and economically feasible if the cost and convenience of service is competitive with alternative available storage methods for peak supply requirements. It is believed that mined cavern storage can provide the advantages of high delivery rates and multiple fill-withdrawal cycles in areas where salt cavern storage is not possible. In this research project, PB-KBB merged advanced mining technologies and gas refrigeration techniques to develop conceptual designs and cost estimates to demonstrate the commercialization potential of the storage of refrigerated natural gas in hard rock caverns. Five regions of the U.S.A. were studied for underground storage development and PB-KBB reviewed the literature to determine if the geology of these regions was suitable for siting hard rock storage caverns. Area gas market conditions in these regions were also studied to determine the need for such storage. Based on an analysis of many factors, a possible site was determined to be in Howard and Montgomery Counties, Maryland. The area has compatible geology and a gas industry infrastructure for the nearby market populous of Baltimore and Washington D.C.. As Gas temperature is lowered, the compressibility of the gas reaches an optimum value. The compressibility of the gas, and the resultant gas density, is a function of temperature and pressure. This relationship can be used to commercial advantage by reducing the size of a storage cavern for a given working volume of natural gas. This study looks at this relationship and and the potential for commercialization of the process in a storage application. A conceptual process design, and cavern design were developed for various operating conditions. Potential site locations were considered and a typical plant layout was developed. In addition a geomechanical review of the proposed cavern design was performed, evaluating the stability of the mine rooms and shafts, and the effects of the refrigerated gas temperatures on the stability of the cavern. Capital and operating cost estimates were also developed for the various temperature cases considered. The cost estimates developed were used to perform a comparative market analysis of this type of gas storage system to other systems that are commercially used in the region of the study.

  11. Analysis on Storage of Spent Nuclear Fuel

    International Nuclear Information System (INIS)

    Interim storage of spent nuclear fuel which has been commercialized as a short-term management alternative in foreign countries is analyzed in this report Based on the location, storage facility is divided into an independent storage facility at reactor(AR) and an interim storage facility away from reactor(AFR). Also, based on the technical characteristics, wet storage and dry storage are described in detail. Especially, various dry storage methods are described in consideration of 20 years of experience. As a result, all of commercialized storage methods may be looked on safe in technical aspect. Additionally, status of interim atorage in USA, Japan, Canada and German is prepared in this study

  12. Energy storage for sustainable microgrid

    CERN Document Server

    Gao, David Wenzhong

    2015-01-01

    Energy Storage for Sustainable Microgrid addresses the issues related to modelling, operation and control, steady-state and dynamic analysis of microgrids with ESS. This book discusses major electricity storage technologies in depth along with their efficiency, lifetime cycles, environmental benefits and capacity, so that readers can envisage which type of storage technology is best for a particular microgrid application. This book offers solutions to numerous difficulties such as choosing the right ESS for the particular microgrid application, proper sizing of ESS for microgrid, as well as

  13. Storage of pork by irradiation

    International Nuclear Information System (INIS)

    In this paper the study of storage of pork, irradiated with Co-60 gamma rays, is recommended. The changes of the appearance and the main qualitative indexes of pork, irradiated with 1.5 M rad radiation and after two month's storage, were analysed. The evaluation of storage, transportation and nutritional acceptability of the two kinds of irradiated pork products was made. Systematic toxicological tests of rats and dogs, fed with irradiated pork, were given. The comparison of the economic facilitation of refrigerated pork and irradiated pork was made. (author)

  14. Energy storage a new approach

    CERN Document Server

    Zito, Ralph

    2010-01-01

    Energy Storage: A New Approach presents practical solutions to the problem of energy storage on a massive scale.  This revolutionary book describes   technologies that include basic chemical concepts that engineers have been practicing for years, but presents new material that could transform the energy industry.  Regardless where power is generated from-oil, natural gas, coal, solar, wind, or any of the other emerging sources- energy storage is something that the industry MUST learn and practice.  With the world energy demand increasing, mostly due to industrial growth in China

  15. Seasonal storage for solar energy

    International Nuclear Information System (INIS)

    Seasonal storage for solar energy have been the object of intensive theoretical studies and practical experiments. Within the frame of the research programme of the International Energy Agency 11 objects were set up and tested in practice. It was the aim of this research and demonstration projects to examine thermal long term storage for the use of solar energy and waste heat from industrial processes. In the following the actual state of the art of long term storage of solar energy for district heating in buildings will be described and possibilities of its use in Austria will be assessed. (author)

  16. Neutron scattering and hydrogen storage

    Directory of Open Access Journals (Sweden)

    A.J. Ramirez-Cuesta

    2009-11-01

    Full Text Available Hydrogen has been identified as a fuel of choice for providing clean energy for transport and other applications across the world and the development of materials to store hydrogen efficiently and safely is crucial to this endeavour. Hydrogen has the largest scattering interaction with neutrons of all the elements in the periodic table making neutron scattering ideal for studying hydrogen storage materials. Simultaneous characterisation of the structure and dynamics of these materials during hydrogen uptake is straightforward using neutron scattering techniques. These studies will help us to understand the fundamental properties of hydrogen storage in realistic conditions and hence design new hydrogen storage materials.

  17. Seasonal Thermal Energy Storage Program

    Science.gov (United States)

    Minor, J. E.

    1980-03-01

    The Seasonal Thermal Energy Storage (STES) Program designed to demonstrate the storage and retrieval of energy on a seasonal basis using heat or cold available from waste or other sources during a surplus period is described. Factors considered include reduction of peak period demand and electric utility load problems and establishment of favorable economics for district heating and cooling systems for commercialization of the technology. The initial thrust of the STES Program toward utilization of ground water systems (aquifers) for thermal energy storage is emphasized.

  18. Groundwater and Terrestrial Water Storage

    Science.gov (United States)

    Rodell, Matthew; Chambers, Don P.; Famiglietti, James S.

    2012-01-01

    Groundwater is a vital resource and also a dynamic component of the water cycle. Unconfined aquifer storage is less responsive to short term weather conditions than the near surface terrestrial water storage (TWS) components (soil moisture, surface water, and snow). However, save for the permanently frozen regions, it typically exhibits a larger range of variability over multi-annual periods than the other components. Groundwater is poorly monitored at the global scale, but terrestrial water storage (TWS) change data from the Gravity Recovery and Climate Experiment (GRACE) satellite mission are a reasonable proxy for unconfined groundwater at climatic scales.

  19. Lightweight hydride storage materials

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, G.J.; Guthrie, S.E.; Bauer, W. [Sandia National Labs., Livermore, CA (United States)

    1995-09-01

    The need for lightweight hydrides in vehicular applications has prompted considerable research into the use of magnesium and its alloys. Although this earlier work has provided some improved performance in operating temperature and pressure, substantial improvements are needed before these materials will significantly enhance the performance of an engineered system on a vehicle. We are extending the work of previous investigators on Mg alloys to reduce the operating temperature and hydride heat of formation in light weight materials. Two important results will be discussed in this paper: (1) a promising new alloy hydride was found which has better pressure-temperature characteristics than any previous Mg alloy and, (2) a new fabrication process for existing Mg alloys was developed and demonstrated. The new alloy hydride is composed of magnesium, aluminum and nickel. It has an equilibrium hydrogen overpressure of 1.3 atm. at 200{degrees}C and a storage capacity between 3 and 4 wt.% hydrogen. A hydrogen release rate of approximately 5 x 10{sup -4} moles-H{sub 2}/gm-min was measured at 200{degrees}C. The hydride heat of formation was found to be 13.5 - 14 kcal/mole-H{sub 2}, somewhat lower than Mg{sub 2}Ni. The new fabrication method takes advantage of the high vapor transport of magnesium. It was found that Mg{sub 2}Ni produced by our low temperature process was better than conventional materials because it was single phase (no Mg phase) and could be fabricated with very small particle sizes. Hydride measurements on this material showed faster kinetic response than conventional material. The technique could potentially be applied to in-situ hydride bed fabrication with improved packing density, release kinetics, thermal properties and mechanical stability.

  20. A Review of Energy Storage Technologies

    DEFF Research Database (Denmark)

    Connolly, David

    2010-01-01

    -alone technology that will be utilised in Ireland for the integration of fluctuating renewable energy. However, the HESS, TESS, and EVs are the also very promising, but require more research to remove uncertainty surrounding their benefits and costs. For some countries, CAES could be a more suitable technology......A brief examination into the energy storage techniques currently available for the integration of fluctuating renewable energy was carried out. These included Pumped Hydroelectric Energy Storage (PHES), Underground Pumped Hydroelectric Energy Storage (UPHES), Compressed Air Energy Storage (CAES......), Battery Energy Storage (BES), Flow Battery Energy Storage (FBES), Flywheel Energy Storage (FES), Supercapacitor Energy Storage (SCES), Superconducting Magnetic Energy Storage (SMES), Hydrogen Energy Storage System (HESS), Thermal Energy Storage (TES), and Electric Vehicles (EVs). The objective was to...

  1. Laser cooling in storage rings

    International Nuclear Information System (INIS)

    The application of the laser cooling technique to longitudinal phase space cooling of stored ion beams is introduced. Results of recent experiments at the ASTRID storage ring are discussed. copyright 1995 American Institute of Physics

  2. Natural gas underground storage facilities

    International Nuclear Information System (INIS)

    Major component of the gas industry, the storage contributes to balancing supply and demand, participates to the security of supply, favors the optimization of the transportation system, and allows to arbitrate gas prices. It represents also one of the most needed solution of modulation. For all these reasons, the implementation of all solutions allowing the non-discriminatory access for all operators is of prime importance. In this paper, the opinions of four experts have been gathered to answer the following questions: what are the regulations in force? How storage capacities are allocated to French operators and in some other European countries? Are storage capacities (both in volume and peak flow rate) enough to meet the demand? What are the operators' projects of development? What are the service and capacity offers of storage facility operators? What is their position with respect to other operators' offer in Europe? (J.S.)

  3. Photovoltaic power systems energy storage

    International Nuclear Information System (INIS)

    Basically, the solar photovoltaic power system consists of: Array of solar panels; Charge/voltage stabilizer; Blocking diode and Storage device. The storage device is a very important part of the system due to the necessity to harmonize the inevitable time shift between energy supply and demand. As energy storage, different devices can be utilized, such as hydropumping, air or other gas compression, flywheel, superconducting magnet, hydrogen generation and so on, but actually secondary (rechargeable) electrochemical cells appear to be the best storage device, due to the direct use for recharge of the d.c. current provided by the solar panels, without any intermediate step of energy transformation and its consequent loss of efficiency

  4. ALICE bags data storage accolades

    CERN Multimedia

    2007-01-01

    ComputerWorld has recognized CERN with an award for the 'Best Practices in Storage' for ALICE's data acquisition system, in the category of 'Systems Implementation'. The award was presented to the ALICE DAQ team on 18 April at a ceremony in San Diego, CA. (Top) ALICE physicist Ulrich Fuchs. (Bottom) Three of the five storage racks for the ALICE Data Acquisition system (Photo Antonio Saba). Between 16 and19 April, one thousand people from data storage networks around the world gathered to attend the biannual Storage Networking World Conference. Twenty-five companies and organizations were celebrated as finalists, and five of those were given honorary awards-among them CERN, which tied for first place in the category of Systems Implementation for the success of the ALICE Data Acquisition System. CERN was one of five finalists in this category, which recognizes the winning facility for 'the successful design, implementation and management of an interoperable environment'. 'Successful' could include documentati...

  5. Advanced spent fuel storage pools

    International Nuclear Information System (INIS)

    Full text: Spent fuel from Power Reactors is currently stored either in at-reactor pools or in independent spent fuel storage installations (ISFSI) using wet or dry storage technology. During the past 15 years, storage capacity of at reactor pools was increased using high density spent fuel storage technology. To achieve maximum capacity, storage racks were replaced in many of the power reactors in operation at least once, some of them went through even various reracking cycles. Independent spent fuel storage installations were established either at the site of power reactors of away from them. They use either storage pools or dry technology, the latter in form of metal casks and concrete silos or vaults. Storage of spent fuel from power reactors must be safe for the public and must protect the environment from its radioactive content. For this purpose, adequate regulation was developed and is available to be applied. However, advances in fuel and core design as well the need for extended storage periods require frequent re-assessment of the available spent fuel storage technology. Improved fuel utilization leads to elevated burn-up resulting in higher heat generation of the spent fuel in the longer term. Also spent MOX fuel generates considerable more heat than spent uranium fuel unloaded from the power reactors 15 years ago. As most of the mechanisms which could endanger fuel integrity are temperature dependent, effective heat removal is one of the challenges spent fuel storage systems have to face. In order to make most efficient use out of high density storage equipment, designer want to take credit from the actual burn-up of the spent fuel. Existing methodology to analyze burn-up credited spent fuel storage racks for criticality safety is being further developed to be applied in the design of dual-purpose casks or multi-purpose-canisters. For poisoned high density storage equipment, long term stability of the material as well as efficient neutron absorption is required. Degrading absorbers which contaminate the coolant of fuel pools and primary circuits and fuel assemblies getting stuck in swollen storage cells are well known problems which already caused considerable headaches to many operators and still continue to do so. Although limited in boron content, borated stainless steel has extensively proved as sufficiently effective and extremely stable neutron poison material. Over and above the basic requirements for maximum safety for operators and the public, logistic in fuel reception is an important aspect to be addressed with the design of independent spent fuel storage facilities. The aspect to keep the operators exposure to radiation as low as reasonably achievable requires expeditious reception of spent fuel and its transfer to the dedicated storage location. Independent wet storage facilities are known for many years to comply best with most of the expectations as described above. However, the assumption to need active cooling systems and to generate secondary waste had caused vendors and users to look favorably at dry storage systems. Despite the remarkable development achieved in dry storage technology, most of the spent fuel generated up to now is stored in fuel pools, either at the reactors or in independent installations. One of the latest achievements in wet storage technology is used in Framatome-ANP's wet storage facility design as currently being performed for the new spent fuel pool building to be constructed at the Goesgen Nuclear Power Station in Switzerland. The advanced design of this independent spent fuel storage facility provides a passive cooling system which reliably removes the heat generated by the spent fuel by natural circulation through air cooled heat exchangers. This progressive design makes extensive use of well balanced safety technology with largely passive safety features developed for Framatome-ANP's Boiling Water Reactor SWR1000. Clearly designed to enable the enforcement of strict foreign material exclusion strategies and state of the art pool water purification equipment reduces operational waste generation to negligible minimum. Due to the passive nature of the operating system, the number of active components which require maintenance is substantially reduced. In addition, due to advanced acquisition methodology of operational data, the frequency of maintenance activities can be determined under consideration of the actual usage. This usually leads to a considerable reduction of human intervention and the time needed to act in radiation areas reducing considerably waste generation and dose burden to personnel. As a facility dedicated to receive and to store fissionable material, regulatory requirements on access controls as defined by the competent regulators are to be met. The advanced wet storage facility design of Framatome-ANP meets safely with all the requirements for safeguards and physical protection including terrorist activities and actions of sabotage. It provides best for safe and reliable storage of spent uranium and MOX fuel for extended periods. Maintenance and repair concepts are available which allow to predict, if correctly applied, such fuel pools to be operable for periods up to 100 years. (author)

  6. Storage of Weapon Plutonium Waste

    International Science & Technology Center (ISTC)

    Development of quantitative criteria of suitability of rock massif for safe long-term storage of waste from weapon Plutonium production illustrated by the underground mining working at Krasnoyarsk Integrated Mining and Chemical Works.

  7. Autumn study on storage rings

    CERN Multimedia

    1974-01-01

    The first two weeks of October have seen storage ring people from accelerator Laboratories throughout the world at CERN to study the fundamental problems of very high energy protonproton colliding beam machines.

  8. Old radioactive waste storage sites

    International Nuclear Information System (INIS)

    After a recall of the regulatory context for the management of old sites used for the storage of radioactive wastes with respect with their activity, the concerned products, the disposal or storage type, this document describes AREVA's involvement in the radioactive waste management process in France. Then, for the different kinds of sites (currently operated sites having radioactive waste storage, storage sites for uranium mineral processing residues), it indicates their location and name, their regulatory status and their control authority, the reference documents. It briefly presents the investigation on the long term impact of uranium mineral processing residues on health and environment, evokes some aspects of public information transparency, and presents the activities of an expertise group on old uranium mines. The examples of the sites of Bellezane (uranium mineral processing residues) and COMURHEX Malvesi (assessment of underground and surface water quality at the vicinity of this installation) are given in appendix

  9. Cost-oblivious storage reallocation

    OpenAIRE

    Bender, Michael A.; Farach-Colton, Martin; Fekete, Sándor P.; Fineman, Jeremy T.; Gilbert, Seth

    2014-01-01

    Databases need to allocate and free blocks of storage on disk. Freed blocks introduce holes where no data is stored. Allocation systems attempt to reuse such deallocated regions in order to minimize the footprint on disk. If previously allocated blocks cannot be moved, the problem is called the memory allocation problem, which is known to have a logarithmic overhead in the footprint. This paper defines the storage reallocation problem, where previously allocated blocks ...

  10. Physical energy storage employed worldwide

    OpenAIRE

    Spataru, C.; Barrett, M.; Kok, Y. C.

    2014-01-01

    The increasing level in renewable energy capacity presents new challenges. In essence, renewables are weather-dependent and inputs such as solar radiation or wind are not constantly available. The integration of energy storage technologies are important to improve the potential for flexible energy demand and ensure that excess renewable energy can be stored for use at a later time. This paper will explore various types of physical energy storage technologies that are currently employed worldw...

  11. Lithium ion storage between graphenes

    OpenAIRE

    Chan Yue; Hill James

    2011-01-01

    Abstract In this article, we investigate the storage of lithium ions between two parallel graphene sheets using the continuous approximation and the 6-12 Lennard-Jones potential. The continuous approximation assumes that the carbon atoms can be replaced by a uniform distribution across the surface of the graphene sheets so that the total interaction potential can be approximated by performing surface integrations. The number of ion layers determines the major storage characteristics of the ba...

  12. Putting TMI 2 into storage

    International Nuclear Information System (INIS)

    The unique condition of the Three Mile Island 2 plant prompted the operator, GPU Nuclear, to propose a special plan for maintaining it in a safe state before decommissioning begins. The plan is called ''monitored storage''. Monitored storage is not a decommissioning plan. Rather, it is a plan that allows TMI 2 to stand in a safe, monitored state until the licence of its sister plant, TMI 1, expires. At that time, GPU Nuclear plans to decommission both plants together. (Author)

  13. Hydrogen Storage In Nanostructured Materials

    OpenAIRE

    Assfour, Bassem

    2011-01-01

    Hydrogen is an appealing energy carrier for clean energy use. However, storage of hydrogen is still the main bottleneck for the realization of an energy economy based on hydrogen. Many materials with outstanding properties have been synthesized with the aim to store enough amount of hydrogen under ambient conditions. Such efforts need guidance from material science, which includes predictive theoretical tools. Carbon nanotubes were considered as promising candidates for hydrogen storag...

  14. Advances in information storage systems

    CERN Document Server

    Bhushan, Bharat

    1995-01-01

    The series Advances in Information Storage Systems covers a wide range of interdisciplinary technical areas, related to magnetic or optical storage systems. The following nonexhaustive list is indicative of the scope of the topics: Friction, Adhesion, Wear and Lubrications, Coatings, Solid Mechanics, Air Flow, Contamination, Instrumentation, Dynamics, Shock and Vibration, Controls, Head and Suspension Design, Actuators, Spindle and Actuator Motors and Bearings, Structure of Thin Films, Corrosion, Long-Term Reliability, Materials and Processing, Manufacturing and Automation, Economics.This volu

  15. Towards a community cloud storage

    OpenAIRE

    Liu, Ying(College of Nuclear Science and Technology, Beijing Normal University, 100875, Beijing, China); Vlassov, Vladimir; Navarro Moldes, Leandro

    2014-01-01

    Community Clouds, usually built upon community networks, operate in a more disperse environment compared to a data center Cloud, with lower capacity and less reliable servers separated by a more heterogeneous and less predictable network interconnection. These differences raise challenges when deploying Cloud applications in a community Cloud. Open Stack Swift is an open source distributed storage system, which provides stand alone highly available and scalable storage from Open Stack Cloud c...

  16. AB Levitator and Electricity Storage

    OpenAIRE

    Bolonkin, Alexander

    2007-01-01

    The author researched this new idea - support of flight by any aerial vehicles at significant altitude solely by the magnetic field of the planet. It is shown that current technology allows humans to create a light propulsion (AB engine) which does not depend on air, water or ground terrain. Simultaniosly, this revolutionary thruster is a device for the storage of electricity which is extracted and is replenished (during braking) from/into the storage with 100 percent efficiency. The relative...

  17. Analysis of cloud storage prices

    OpenAIRE

    Mastroeni, Loretta; Naldi, Maurizio

    2012-01-01

    Cloud storage is fast securing its role as a major repository for both consumers and business customers. Many companies now offer storage solutions, sometimes for free for limited amounts of capacity. We have surveyed the pricing plans of a selection of major cloud providers and compared them using the unit price as the means of comparison. All the providers, excepting Amazon, adopt a bundling pricing scheme; Amazon follows instead a block-declining pricing policy. We compare the pricing plan...

  18. An alternative scalable storage system

    OpenAIRE

    LIU, CHUNHUI

    2009-01-01

    With the development of computer processor, the Input/Output (I/O) gap between the Central Processing Unit (CPU) and the storage system widens. The storage system becomes the I/O bottleneck of the whole system. Solving this problem is a popular topic for many researchers. Redundant Array of Independent / Inexpensive Disks (RAID) is a widely used technique to handle this problem nowadays. Many RAID products are available on the market. However, for small companies, these products are too expen...

  19. Data Storage Control System Design

    OpenAIRE

    Yury Yurievich Shumilov; Nikolay Sergeevich Dudakov

    2014-01-01

    The paper presents a methodology for evaluating and improving the effectiveness of storage management during the development of automated control systems. The description of the storage management system in terms of queuing theory is proposed. The model of the system and the criteria for efficient processing of requests to read and write data are provided. The authors also propose the partitioning of stored data and the use of several software solutions to improve the system performance.

  20. A concept of an electricity storage system with 50 MWh storage capacity

    Directory of Open Access Journals (Sweden)

    Józef Paska

    2012-06-01

    Full Text Available Electricity storage devices can be divided into indirect storage technology devices (involving electricity conversion into another form of energy, and direct storage (in an electric or magnetic fi eld. Electricity storage technologies include: pumped-storage power plants, BES Battery Energy Storage, CAES Compressed Air Energy Storage, Supercapacitors, FES Flywheel Energy Storage, SMES Superconducting Magnetic Energy Storage, FC Fuel Cells reverse or operated in systems with electrolysers and hydrogen storage. These technologies have diff erent technical characteristics and economic parameters that determine their usability. This paper presents two concepts of an electricity storage tank with a storage capacity of at least 50 MWh, using the BES battery energy storage and CAES compressed air energy storage technologies.