WorldWideScience
1

11S Storage globulin from pumpkin seeds: regularities of proteolysis by papain.  

Science.gov (United States)

Limited proteolysis of the ?- and ?-chains and deep cleavage of the ??-subunits by the cooperative (one-by-one) mechanism was observed in the course of papain hydrolysis of cucurbitin, an 11S storage globulin from seeds of the pumpkin Cucurbita maxima. An independent analysis of the kinetics of the limited and cooperative proteolyses revealed that the reaction occurs in two successive steps. In the first step, limited proteolysis consisting of detachments of short terminal peptides from the ?- and ?-chains was observed. The cooperative proteolysis, which occurs as a pseudo-first order reaction, started at the second step. Therefore, the limited proteolysis at the first step plays a regulatory role, impacting the rate of deep degradation of cucurbitin molecules by the cooperative mechanism. Structural alterations of cucurbitin induced by limited proteolysis are suggested to generate its susceptibility to cooperative proteolysis. These alterations are tentatively discussed on the basis of the tertiary structure of the cucurbitin subunit pdb|2EVX in comparison with previously obtained data on features of degradation of soybean 11S globulin hydrolyzed by papain. PMID:25365492

Rudakova, A S; Rudakov, S V; Kakhovskaya, I A; Shutov, A D

2014-08-01

2

Gene families encoding isoforms of two major sesame seed storage proteins, 11S globulin and 2S albumin.  

Science.gov (United States)

Sesame (Sesamum indicum L.) seed has been recognized as a nutritional protein source owing to its richness in methionine. Storage proteins have been implicated in allergenic responses to sesame consumption. Two abundant storage proteins, 11S globulin and 2S albumin, constitute 60-70 and 15-25% of total sesame proteins, respectively. Two gene families separately encoding four 11S globulin and three 2S albumin isoforms were identified in a database search of 3328 expressed sequence tag (EST) sequences from maturing sesame seeds. Full-length cDNA sequences derived from these two gene families were completed by PCR using a maturing sesame cDNA library as the template. The amino acid compositions of these deduced storage proteins revealed that the richness in methionine is attributed mainly to two 2S albumin isoforms and partly to one 11S globulin isoform. The presence of four 11S globulin and three 2S albumin isoforms resolved in SDS-PAGE was confirmed by MALDI-MS analyses. The abundance of these isoforms was in accord with the occurrence frequency of their EST sequences in the database. A comprehensive understanding of these storage proteins at the molecular level may also facilitate the identification of allergens in crude sesame products that have caused severe allergic reactions increasingly reported in the past decade. PMID:17147444

Hsiao, Eric S L; Lin, Li-Jen; Li, Feng-Yin; Wang, Miki M C; Liao, Ming-Yuan; Tzen, Jason T C

2006-12-13

3

Proteolytic Cleavage at Twin Arginine Residues Affects Structural and Functional Transitions of Lupin Seed 11S Storage Globulin.  

Science.gov (United States)

The 11S storage globulin of white lupin seeds binds to a metal affinity chromatography matrix. Two unusual stretches of contiguous histidine residues, reminiscent of the multiple histidines forming metal binding motifs, at the C-terminal end of 11S globulin acidic chains were hypothesized as candidate elements responsible for the binding capacity. To prove this, the protein was incubated with a lupin seed endopeptidase previously shown to cleave at twin arginine motifs, recurrent in the sequence region of interest. Upon incubation with this enzyme, the loss of metal binding capacity paralleled that of the anti-his-tag reactive polypeptides. The recovered small proteolytic fragment was analyzed by mass spectrometry and N-terminal sequencing and found to correspond to the 24-mer region cleaved off at twin arginine residues and containing the natural his-tag-like region. Similarly, when lupin seeds were germinated for a few days, the his-tag containing 11S globulin chain was converted to a form devoid of such region, suggesting that this mechanism is a part of the natural degradatory process of the protein. The hypothesis that the ordered and controlled dismantling of storage proteins may generate peptide fragments with potential functional roles in plant ontogenesis is presented and discussed. PMID:25658355

Capraro, Jessica; Sessa, Fabio; Magni, Chiara; Scarafoni, Alessio; Maffioli, Elisa; Tedeschi, Gabriella; Croy, Ron R D; Duranti, Marcello

2015-01-01

4

Proteolytic Cleavage at Twin Arginine Residues Affects Structural and Functional Transitions of Lupin Seed 11S Storage Globulin  

Science.gov (United States)

The 11S storage globulin of white lupin seeds binds to a metal affinity chromatography matrix. Two unusual stretches of contiguous histidine residues, reminiscent of the multiple histidines forming metal binding motifs, at the C-terminal end of 11S globulin acidic chains were hypothesized as candidate elements responsible for the binding capacity. To prove this, the protein was incubated with a lupin seed endopeptidase previously shown to cleave at twin arginine motifs, recurrent in the sequence region of interest. Upon incubation with this enzyme, the loss of metal binding capacity paralleled that of the anti-his-tag reactive polypeptides. The recovered small proteolytic fragment was analyzed by mass spectrometry and N-terminal sequencing and found to correspond to the 24-mer region cleaved off at twin arginine residues and containing the natural his-tag-like region. Similarly, when lupin seeds were germinated for a few days, the his-tag containing 11S globulin chain was converted to a form devoid of such region, suggesting that this mechanism is a part of the natural degradatory process of the protein. The hypothesis that the ordered and controlled dismantling of storage proteins may generate peptide fragments with potential functional roles in plant ontogenesis is presented and discussed. PMID:25658355

Capraro, Jessica; Sessa, Fabio; Magni, Chiara; Scarafoni, Alessio; Maffioli, Elisa; Tedeschi, Gabriella; Croy, Ron R. D.; Duranti, Marcello

2015-01-01

5

Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean  

OpenAIRE

During seed development and maturation, large amounts of storage proteins are synthesized and deposited in protein storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via immunogold electron microscopy in developing mung bean cotyledons. It is demonstrate...

Wang, Junqi; Tse, Yu Chung; Hinz, Giselbert; Robinson, David G.; Jiang, Liwen

2011-01-01

6

Serum globulin electrophoresis  

Science.gov (United States)

Globulin electrophoresis ... This test is done to look at globulin proteins in the blood. Identifying the types of globulins can help diagnose certain medical problems. Globulins are roughly divided into three groups: alpha, ...

7

The molecular biology and biochemistry of rice endosperm ?-globulin  

International Nuclear Information System (INIS)

The author's first objective was to isolate a cDNA clone that encodes the rice endosperm ?-globulin. Purified antibodies against a rice storage protein, ?-globulin, were used to screen a ?gt11 cDNA expression library constructed from immature rice seed endosperm. The cDNA insert of clone 4A1 (identified by antibody screening) was used as a probe to identify long cDNA inserts in the library. The deduced amino acid sequence of clone A3-12 cDNA insert (identified by cDNA screening) contained the amino acid sequences of three cyanogen bromide peptides fragment of ?-globulin. The calculated molecular weight and amino acid composition of the deduced amino acid sequence were similar to the ?-globulin protein. Northern blot analysis indicated that mRNA of one size, approximately 1.0 kb, is expressed. Southern genomic blot analysis revealed one band with EcoRI or Hind III digestion. Cell-free translation and immunoprecipitation showed that the initial translation product is approximately 2,000 daltons larger than the mature protein. The amino acid sequence of ?-globulin revealed limited regions of similarities with wheat storage proteins. The author concludes that the cDNA insert in clone A3-12 contained the entire coding region of ?-globulin protein and that ?-globulin is encoded by a single gene. My second objective was to inhibit the degradation of ?-globulin in the salt extract of rice flour. The salt extract of rice flour contained an acid protease whose optimal d an acid protease whose optimal pH was 3 for 3H-casein hydrolysis. A polypeptide with molecular weight of 20,000 was immunologically reactive with ?-globulin antibodies and is produced by limited proteolysis in the extract. Pepstatin inhibited the proteolysis of 3H-casein and slowed the proteolysis of ?-globulin

8

Alpha globulin injections and decreased gamma globulin production in chickens.  

Science.gov (United States)

Electrophoretic analysis shows a strong similarity in the blood serums of chickens subjected to egg parabiosis and of chickens repeatedly injected with fraction IV (alpha globulins). In both instances there is an evident rise in the albumin-plus alpha(1) titer and an evident decrease in the gamma globulin levels. These findings suggest that the injection of alpha globulins has the same serum effect on the animal as parabiotically induced blood chimerism, namely, a reduction in circulating gamma globulin (immunoglobulins?). PMID:4162096

Kamrin, B B

1966-09-01

9

The molecular biology and biochemistry of rice endosperm. alpha. -globulin  

Energy Technology Data Exchange (ETDEWEB)

The author's first objective was to isolate a cDNA clone that encodes the rice endosperm {alpha}-globulin. Purified antibodies against a rice storage protein, {alpha}-globulin, were used to screen a {lambda}gt11 cDNA expression library constructed from immature rice seed endosperm. The cDNA insert of clone 4A1 (identified by antibody screening) was used as a probe to identify long cDNA inserts in the library. The deduced amino acid sequence of clone A3-12 cDNA insert (identified by cDNA screening) contained the amino acid sequences of three cyanogen bromide peptides fragment of {alpha}-globulin. The calculated molecular weight and amino acid composition of the deduced amino acid sequence were similar to the {alpha}-globulin protein. Northern blot analysis indicated that mRNA of one size, approximately 1.0 kb, is expressed. Southern genomic blot analysis revealed one band with EcoRI or Hind III digestion. Cell-free translation and immunoprecipitation showed that the initial translation product is approximately 2,000 daltons larger than the mature protein. The amino acid sequence of {alpha}-globulin revealed limited regions of similarities with wheat storage proteins. The author concludes that the cDNA insert in clone A3-12 contained the entire coding region of {alpha}-globulin protein and that {alpha}-globulin is encoded by a single gene. My second objective was to inhibit the degradation of {alpha}-globulin in the salt extract of rice flour. The salt extract of rice flour contained an acid protease whose optimal pH was 3 for {sup 3}H-casein hydrolysis. A polypeptide with molecular weight of 20,000 was immunologically reactive with {alpha}-globulin antibodies and is produced by limited proteolysis in the extract. Pepstatin inhibited the proteolysis of 3H-casein and slowed the proteolysis of {alpha}-globulin.

Shorrosh, B.S.

1989-01-01

10

Vicilins (7S storage globulins of cowpea (Vigna unguiculata seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae larvae  

Directory of Open Access Journals (Sweden)

Full Text Available The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata seed vicilins (7S storage proteins bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae.

Sales M.P.

2001-01-01

11

Serum globulines as radioprotective substances  

International Nuclear Information System (INIS)

It is established on the basis of literary and experimental data that the preparations of globulin increase the survivability of irradiated animals and contribute to the normalization of the protective function of the immune system

12

Antibodies from a patient with type 1 diabetes and celiac disease bind to macrophages that express the scavenger receptor CD163.  

Science.gov (United States)

Antibodies against the wheat storage globulin Glo-3A from a patient with both type 1 diabetes (T1D) and celiac disease were enriched to identify potential molecular mimicry between wheat antigens and T1D target tissues. Recombinant Glo-3A was used to enrich anti-Glo-3A immunoglobulin G antibodies from plasma by batch affinity chromatography. Rat jejunum and pancreas, as well as human duodenum and monocytes were probed, and binding was evaluated by immunohistochemistry and confocal microscopy. Glo-3A-enriched antibodies bound to a specific subset of cells in the lamina propria of rat jejunum that co-localized mostly with a marker of resident, alternatively activated CD163-positive (CD163?) macrophages. Blood monocytes and macrophage-like cells in human duodenum were also labelled with the enriched antibodies. Blocking studies revealed that binding to CD163? macrophages was not due to cross-reactivity with anti-Glo-3A antibodies, but rather to non-Glo-3A antibodies co-purified during antibody enrichment. The novel finding of putative autoantibodies against tolerogenic intestinal CD163? macrophages suggests that regulatory macrophages were targeted in this patient with celiac disease and T1D. PMID:21766094

Sonier, Brigitte; Strom, Alexander; Wang, Gen-Sheng; Patrick, Christopher; Crookshank, Jennifer A; Mojibian, Majid; MacFarlane, Amanda J; Scott, Fraser W

2011-06-01

13

Post-gamma globulin. II  

International Nuclear Information System (INIS)

Post-gamma globulin previously isolated and partially sequenced in this laboratory was used for production of polyclonal and monoclonal (hybridoma) antibodies. A radioimmunoassay method was developed for quantitation of post-gamma globulin with either antibody. The titration curves obtained were treated statistically and found practically indistinguishable. The sensitivity of the method adopted for the quantitation of post-gamma globulin in a variety of biological fluids was 0.13 ng/ml and the upper limit of precision was 2.5 ng/ml. The following results were obtained (mean +- 1 SD): normal sera, 0.96 +- 0.20 ?g/ml; pregnancy sera, 1.08 +- 0.28 ?g/ml; cord blood 2.08 +- 0.33 ?/g/ml; hospitalized patient's sera, 1.3 +- 0.54 ?g/ml; geriatric subjects' sera 2.26 +- 1.10 ?g/ml; cerebrospinal fluid, 5.37 +- 3.36 ?g/ml; saliva, 1.22 +- 0.67 ?g/ml; synovial fluid, 1.27 +- 0.41 ?g/ml and urine, 0.11 +- 0.125 ?g/ml. To shed light on the catabolism of post-gamma globulin the levels of #betta#2-microglobulin were also measured radiometrically. Correlative statistical analysis of all the data have shown that renal handling of post-gamma globulin and #betta#2-microglobulin may be very similar but not necessarily identical. (Auth.)

14

Purification of equine Gc-globulin  

DEFF Research Database (Denmark)

Objectives With the aim of producing antibodies for an equine Group specific component (Gc)-globulin assay, the protein was purified from normal equine plasma. Methods Equine Gc-globulin was purified from healthy horse plasma using ion exchange chromatography (Q-Sepharose, CM-Sepharose) and preparative PAGE. Results Equine Gc-globulin has successfully been purified from healthy horse plasma and rabbits and mice are being immunized to produce specific antibodies. Conclusions Purification of equine Gc-globulin and the production of specific antibodies will make it possible to develop an assay for measuring Gc-globulin in horses. Studies in rodents and humans have shown that Gc-globulin is a multifunctional acute phase plasma protein, which removes actin from the blood by binding it and facilitating its clearance from the circulation by the liver. As such, Gc-globulin prevents hyper coagulation, shock and death in human patients with massive actin release caused by severe tissue injuries like physical trauma, sepsis, endotoxemia, or liver failure. Gc-globulin is consumed in this process, and the plasma concentration of free Gc-globulin hence decreases rapidly after tissue injury and has shown to be a sensitive marker of acute tissue injury and fatal outcome in humans. Patients with a low plasma concentration of Gc-globulin due to severe tissue injury might potentially benefit from infusions with purified Gc-globulin [1]. With an equine Gc-globulin assay, future studies will investigate the concentration of Gc-globulin in colic horses with intestinal ischemia were Gc-globulin might be useful as a diagnostic and prognostic marker. Horses with intestinal ischemia often die, despite of expensive surgical treatment, because of endotoxemia and shock, therefore these horses potentially could benefit from Gc-globulin infusions.

Houen, Gunnar; Pihl, Tina Holberg

15

Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues  

OpenAIRE

This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured cells, the 8S globulin located to structures that were neither Golgi nor pre-vacuolar compartments (PV...

Wang, Junqi; Shen, Jinbo; Cai, Yi; Robinson, David G.; Jiang, Liwen

2013-01-01

16

Purification of equine Gc-globulin  

OpenAIRE

Objectives With the aim of producing antibodies for an equine Group specific component (Gc)-globulin assay, the protein was purified from normal equine plasma. Methods Equine Gc-globulin was purified from healthy horse plasma using ion exchange chromatography (Q-Sepharose, CM-Sepharose) and preparative PAGE. Results Equine Gc-globulin has successfully been purified from healthy horse plasma and rabbits and mice are being immunized to produce specific antibodies. Conclusions Purification of eq...

Houen, Gunnar; Pihl, Tina Holberg; Andersen, Pia Haubro; Jacobsen, Stine; Olsen, Dorthe Tange

2010-01-01

17

Sex hormone binding globulin in breast cancer  

OpenAIRE

The present study was undertaken to determine the significance of sex hormone binding globulin, the major and specific binding protein for testosterone and estradiol, in breast cancer. Among breast cancer patients, lower serum levels of Sex hormone binding globulin and higher levels of testosterone were observed. Sex hormone binding globulin showed an inverse relationship with testosterone and total cholesterol, and a direct relation with HDL-cholesterol. By the western blot analyses, Sex hor...

Naik S L, Dayalu; Hedau, Suresh; Bahadur, Anil Kumar; Saha, Renuka; Kaur, Sudershan; Ray, Amitabha

2008-01-01

18

Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues.  

Science.gov (United States)

This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured cells, the 8S globulin located to structures that were neither Golgi nor pre-vacuolar compartments (PVCs). Immunogold electron microscopy of the transgenics reveals the 8S globulin-positive structures to be small, spherical, ribosome-covered endoplasmic reticulum (ER)-derived bodies. In BY-2 cells and all vegetative cells, the 8S globulin was present as a pro-form. However, in Arabidopsis embryos, with the onset of endogenous storage protein synthesis, the 8S globulin exited the ER and passed through the PVC to the protein storage vacuole where it was processed to its smaller mature form. These results clearly demonstrated that, when taken out of context and expressed in vegetative cells, the mung bean 8S storage globulin cannot exit the ER, and indicate that natural targeting of storage proteins to the vacuole should be better studied in the maturing seed. PMID:23382549

Wang, Junqi; Shen, Jinbo; Cai, Yi; Robinson, David G; Jiang, Liwen

2013-04-01

19

Crystal structure of soybean 11S globulin: Glycinin A3B4 homohexamer  

OpenAIRE

Most plant seeds contain 11S globulins as major storage proteins for their nutrition. Soybean glycinin belongs to the 11S globulin family and consists of five kinds of subunits. We determined the crystal structure of a homohexamer of the glycinin A3B4 subunit at 2.1-Å resolution. The crystal structure shows that the hexamer has 32-point group symmetry formed by face-to-face stacking of two trimers. The interface buries the highly conserved interchain disulfide. Based on the structure, ...

Adachi, Motoyasu; Kanamori, Jiro; Masuda, Taro; Yagasaki, Kazuhiro; Kitamura, Keisuke; Mikami, Bunzo; Utsumi, Shigeru

2003-01-01

20

Regulation of the globulins of lupin seed  

International Nuclear Information System (INIS)

Seeds of Lupinus angustifolius contain three globulins, conglutins ?, ?, ? with methionine contents of 0.2, 0.0 and 1.3%, which can be resolved and estimated by electrophoresis on cellulose acetate. It is suggested that a significant improvement in sulphur-amino acid content of lupin seed could be achieved by selecting varieties rich in conglutins ? and ?. This lupin, grown in the virtual absence of sulphate and visibly sulphur deficients, produces viable seeds with about the normal protein content but with most of the usual conglutins ? and ? replaced by conglutin ?. As would be expected, these seeds are very low in methionine because they contain mostly conglutin ?. This appears to be the first report of a dramatic change in the proportions of individual legume globulins following manipulation of plant nutrition. (author)

21

Intravenous immune globulin in chronic lymphocytic leukaemia.  

OpenAIRE

The most common complication of chronic lymphocytic leukaemia (CLL) is infection, which occurs mainly in advanced stages of disease or in those patients with hypogammaglobulinaemia. Intravenous immune globulin (IVIG) has been shown to be a useful prophylactic therapy against infections in such patients. A randomized, double-blind study on 36 patients receiving either 500 mg/kg or 250 mg/kg IVIG every 4 weeks was undertaken to determine the dose regimen required. There was no significant diffe...

Gamm, H.; Huber, C.; Chapel, H.; Lee, M.; Ries, F.; Dicato, Ma

1994-01-01

22

Antithymocyte globulin stimulates human hematopoietic progenitor cells.  

OpenAIRE

Antithymocyte globulin (ATG), a horse antihuman thymus antiserum highly effective in the majority of patients with aplastic anemia, was studied for its in vitro effects on hematopoietic progenitor cells. Marrow cells isolated by an immunoadherence technique with the HPCA-1 (human progenitor cell antigen) monoclonal antibody after removal of contaminating T cells and macrophages formed erythroid colonies in methyl cellulose media in the presence of ATG at concentrations of 25-50 micrograms/ml....

Huang, A. T.; Mold, N. G.; Zhang, S. F.

1987-01-01

23

Genetic variation in pea seed globulin composition  

OpenAIRE

A quantitative characterization of seeds from 59 pea (Pisum sativum L.) lines and relative taxa with various external characteristics and wide geographical origin was performed to explore the genetic variation of pea concerning its starch and protein contents and globulin composition. Pea lines, which produce round, wrinkled, flat, and round-dimpled seeds, have starch as the major reserve, with an average content of 46%. Protein content varied from 13.7 to 30.7% of the seed dry matter, with a...

Tzitzikas, E.; Vincken, J. P.; Groot, J.; Gruppen, H.; Visser, R. G. F.

2006-01-01

24

Crystallization of seed globulins from legumes.  

Science.gov (United States)

Seeds contain large quantities of proteins and are therefore main food sources. In the last century, protein extracts of legume seeds were dialysed against distilled water and in some cases small crystals of pure protein appeared. However, those crystals were generally of poor quality with respect to X-ray diffraction. Recently, the crystallization of some of them was improved and the structures of two 7S globulins, phaseolin from Phaseolus vulgaris and canavalin from Canavalia ensiformis, have been determined at 3.0 and 2.6 A resolution, respectively. Efforts to improve the quality of the phaseolin crystals resulted in three new crystal forms which will be discussed in this paper. The only high-resolution X-ray analysis of a seed globulin from legumes is that of narbonin, a 2S protein from Vicia narbonensis. The crystal structure at 1.8 A shows a very compact packing in layers of molecules. The intermolecular contacts include salt bridges and hydrophobic clusters that might facilitate both the aggregation of the molecules and their crystallization. Because the seed globulins appear in large quantities in the protein bodies of the seeds, efficient packing of the molecules similar to the crystal packing can be assumed. PMID:15299427

Hennig, M; Schlesier, B

1994-07-01

25

Sex hormone binding globulin and corticosteroid binding globulin as major effectors of steroid action.  

Science.gov (United States)

Contrary to the long-held postulate of steroid-hormone binding globulin action, these protein carriers of steroids are major players in steroid actions in the body. This manuscript will focus on our work with sex hormone binding globulin (SHBG) and corticosteroid binding globulin (CBG) and demonstrate how they are actively involved in the uptake, intracellular transport, and possibly release of steroids from cells. This manuscript will also discuss our own findings that the steroid estradiol is taken up into the cell, as demonstrated by uptake of fluorescence labeled estradiol into Chinese hamster ovary (CHO) cells, and into the cytoplasm where it may have multiple actions that do not seem to involve the cell nucleus. This manuscript will focus mainly on events in two compartments of the cell, the plasma membrane and the cytoplasm. PMID:24269740

Caldwell, Jack D; Jirikowski, Gustav F

2014-03-01

26

Radioimmune assay of heterologous serum ?-globulin in patients receiving rabbit antihuman thymocyte globulin  

International Nuclear Information System (INIS)

A radioimmune assay (RIA) method for detecting heterologous serum rabbit ?-globulin (RG) and antibody to this protein is described. The methodology is used for monitoring serum levels of rabbit globulin in patients receiving rabbit ATG (RATG). In 7 cardiac recipients receiving RATG, maximum serum levels of RG were achieved 1 to 3 days after administration of final dose. RG half-life subsequent to peak serum levels was rapid (X = 36 hr) in 4 patients and prolonged (X = 18 days) in 3 patients. Patient antibody to rabbit ?-globulin was detectable only in those patients with short RG half-life. Antirabbit antibody titers in these patients were extremely low and barely detectable by RIA

27

21 CFR 862.1330 - Globulin test system.  

Science.gov (United States)

...ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems § 862.1330 Globulin test system. (a)...

2010-04-01

28

21 CFR 862.1330 - Globulin test system.  

Science.gov (United States)

...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems § 862.1330 Globulin... (b) Classification. Class I (general controls). The device is exempt...

2010-04-01

29

Thyroxine-binding globulin: investigation of microheterogeneity  

OpenAIRE

Preparations of T4-binding globulin (TBG) from human serum was performed using only two affinity chromatography steps. Purity of the protein was demonstrated by a single band in overloaded disc and sodium dodecyl sulfate electrophoresis, equimolar binding to T4, and linearity in sedimentation velocity run. The molecular weight was calculated to be 60,000 +/- 3,000 daltons (n = 3), the sedimentation coefficient was 3.95S, and the Stokes' radius was 37 A. The amino acid composition was found to...

Ga?rtner, Roland; Henze, R.; Horn, K.; Pickardt, C. R.; Scriba, Peter Christian

1981-01-01

30

Purified equine rabies immune globulin: a safe and affordable alternative to human rabies immune globulin.  

OpenAIRE

Reported are the results of a retrospective study of 3156 patients who were treated at the Queen Saovabha Memorial Institute, Bangkok, with equine rabies immune globulin (ERIG). Only 51 patients (1.6%) exhibited serum-sickness-like reactions, none of which persisted for more than a week, and only 8 of these patients (15%) were treated with a short course of steroids. One patient, whose skin test was negative, had an immediate anaphylactic reaction to ERIG that responded to parenteral therapy ...

Wilde, H.; Chomchey, P.; Punyaratabandhu, P.; Phanupak, P.; Chutivongse, S.

1989-01-01

31

Plasma Gamma Globulin Levels After Splenectomy and Spleen Salvage  

OpenAIRE

A series of plasma globulin studies was carried out on 108 patients who were operated on for splenic trauma during the last 3 years. The reasons for splenectomy or spleen salvage were; gunshot wounds in 22 patients (20.3%); stab injuries in 10 patients (9.2%) and blunt abdominal trauma in 76 patients (70.3%). Plasma gamma globulin determinations were made on the 8th postoperative day and at 3 months. In the splenectomy group; plasma gamma globulin determinations demonstrated a sig...

Yasar, Yesilkaya; Zeki, Yilmaz; Ahmet, Erkilic; Metin, Sen; Hu?seyin, Kilic

1989-01-01

32

Calculated globulin (CG) as a screening test for antibody deficiency.  

Science.gov (United States)

Calculated globulin (total protein - albumin) is usually tested as part of a liver function test profile in both primary and secondary care and determines the serum globulin concentration, of which immunoglobulins are a major component. The main use hitherto of calculated globulin is to detect paraproteins when the level is high. This study investigated the potential to use low levels of calculated globulin to detect antibody deficiency. Serum samples with calculated globulin cut-off?globulin with defined cut-off values detects both primary and secondary antibody deficiency and new paraproteins associated with immune-paresis. It is cheap, widely available and under-utilized. Antibody-deficient patients have been discovered using information from calculated globulin values, shortening diagnostic delay and time to treatment with immunoglobulin replacement therapy. PMID:24784320

Jolles, S; Borrell, R; Zouwail, S; Heaps, A; Sharp, H; Moody, M; Selwood, C; Williams, P; Phillips, C; Hood, K; Holding, S; El Shanawany, T

2014-09-01

33

Radiation exposure and serum protein. cap alpha. ,. beta. globulin fraction  

Energy Technology Data Exchange (ETDEWEB)

In a cohort of 4,690 A-bomb survivors receiving physical examinations during the period October 1984 to September 1986 in Hiroshima, protein fractions were analyzed in relation to radiation exposure. The subjects were classified as those receiving 100 rad or more (exposed group) and those receiving 0 rad (control group). Decreased levels of albumin and increased levels of ..cap alpha../sub 1/, ..cap alpha../sub 2/ and ..gamma.. globulins were observed with aging in both men and women. There was no difference in albumin and ..gamma.. globulin levels between the exposed and control groups. Levels of ..cap alpha../sub 1/, ..cap alpha../sub 2/ and ..beta.. globulins, and the percentage of individuals over one SD of protein levels tended to be higher in the exposed group than the control group. These tendencies were noticeable in both male and female younger persons, and as a whole for women. In a multiple regression analysis, there was correlation between exposure doses and the levels of ..cap alpha../sub 1/, ..cap alpha../sub 2/, and ..beta.. globulins; however, the correlation was not seen for albumin and ..gamma.. globulin levels. (Namekawa, K.).

Neriishi, Kazuo; Matsuo, Tatsuki; Ishimaru, Toranosuke; Hosoda, Yutaka

1986-11-01

34

Binding of THS by normal and TSI-containing globulins  

International Nuclear Information System (INIS)

125I-TSH was found to be specifically bound to globulins from healthy people and from patients with Graves' disease (GSI negative). Bsub(max) = 1.2 pmol.mg-1, Ksub(d) = 1.2 mg.pmol-1 was found for TSI negative globulins from normal sera and Bsub(max) = 32 pmol.mg-1, and Ksub(d) = 53 mg.pmol-1 for TSI positive sera. Maximum binding appeared within a 1 h incubation period and was little dependent on temperature. The pH optimum for binding was found between 7 to 8. Linear correlation was found between the protein content and the specific binding of the globulin preparations. LH, FSH, ACTH, prolactin and insulin competed with TSH for the binding sites only in relatively very high concentrations. The binding sites appeared to be macromolecules, since they were eluted from Sephadex G-200 at the void volume. (author)

35

Characterisation of different digestion susceptibility of lupin seed globulins.  

Science.gov (United States)

This study describes in vitro digestion of lupin seed globulins by pancreatin, trypsin and chymotrypsin. Lupin seed globulins turned out to be almost totally susceptible to chymotrypsin digestion. When panceratin or trypsin were used for digestion of lupin seed globulins, ?-conglutin appeared to be resistant to proteolysis. Different fluorescence spectroscopic methods such as fluorescence anisotropy, fluorescence lifetimes and fluorescence quenching measurements were used for detailed characterisation of this phenomenon. A potential reason for ?-conglutin insensitivity to digestion may be related to the fact that lysine, as well as arginine, are positively charged at cell physiological pH. Simultaneously, flavonoids at this pH are partially ionised, which may lead to the occurrence of ionic interactions between these molecules at pH 7.5. The confirmation of this explanation may be the fact that ?-conglutin and vitexin form a static complex, which was observed using fluorescence quenching measurements. PMID:24054261

Czubinski, Jaroslaw; Dwiecki, Krzysztof; Siger, Aleksander; Neunert, Grazyna; Lampart-Szczapa, Eleonora

2014-01-15

36

Efficacy of Human Hyperimmune Globulin in Prevention of Haemophilus influenzae Type b Disease in Infant Rats  

OpenAIRE

To determine the protective efficacy of human hyperimmune globulin to Haemophilus influenzae type b disease in an infant rat model, we compared hyperimmune globulin containing 600 ?g of anti-polyribophosphate (PRP) antibody per ml to conventional immune globulin containing 66 ?g of anti-PRP antibody per ml. The hyperimmune globulin was fractionated from the pooled plasma of 55 adult donors immunized with PRP, the capsular polysaccharide of H. influenzae type b. The disappearance of passivel...

Ambrosino, Donna; Schreiber, John R.; Daum, Robert S.; Siber, George R.

1983-01-01

37

LENTIL TANNIN-GLOBULIN INTERACTIONAND AND IN VITRO HYDROLYSIS  

OpenAIRE

Protein fractions were isolated from lentil cotyledons and tannins were isolated and purified from lentil seed coats. The globulin fraction corresponded to 42.7% of the total lentil flour nitrogen, representing the major protein fraction. Acetone:water (7:3) was the best extractant for seed coat tannins compared to methanol or methanol-HCl 1%. Native and heated (99oC/15 min.) isolated lentil globulin and casein were hydrolyzed with trypsin and pepsin in the absence of tannins and at 1:40, 1:2...

Neves, Valdir A.; Lourenc?o, Euclides J.

1998-01-01

38

Congenital hypothyroidism with hereditary, raised thyroxine binding globulin.  

OpenAIRE

A boy with congenital hypothyroidism and hereditary raised thyroxine binding globulin is described. This hitherto unreported combination resulted in under treatment of the thyroid deficiency until serum thyroid stimulating hormone measurement became routinely available. Inadequate L-thyroxine replacement treatment between 2 and 7 years of age caused retarded bone maturation, poor growth velocity, and probably added to his educational difficulties.

Archer, L. N.; O Malley, B. P.; Swift, P. G.

1985-01-01

39

21 CFR 660.50 - Anti-Human Globulin.  

Science.gov (United States)

The source of this product shall be either serum from animals immunized with one or more human serum globulins or protein-rich fluids derived from stable immunoglobulin-secreting cell lines maintained either in tissue cultures or in secondary hosts. [50 FR 5579,...

2010-04-01

40

Immune Globulin Subcutaneous (Human) 20%: In Primary Immunodeficiency Disorders  

OpenAIRE

Immune globulin subcutaneous 20% is a new high-concentration (200 g/L) solution of highly purified human IgG (?98%) indicated in the EU and the US for antibody replacement therapy in patients with primary immunodeficiency with antibody deficiency, and in the EU for replacement therapy in humoral immunodeficiency secondary to myeloma or chronic lymphocytic leukaemia.

Mccormack, Paul L.

2012-01-01

41

Treatment of neonatal sepsis with intravenous immune globulin  

DEFF Research Database (Denmark)

Neonatal sepsis is a major cause of death and complications despite antibiotic treatment. Effective adjunctive treatments are needed. Newborn infants are relatively deficient in endogenous immunoglobulin. Meta-analyses of trials of intravenous immune globulin for suspected or proven neonatal sepsis suggest a reduced rate of death from any cause, but the trials have been small and have varied in quality.

Brocklehurst, Peter; Farrell, Barbara

2011-01-01

42

Horse antithymocyte globulin as salvage therapy after rabbit antithymocyte globulin for severe aplastic anemia.  

Science.gov (United States)

The effectiveness of salvage therapy for aplastic anemia patients unresponsive to initial rabbit antithymocyte globulin (r-ATG) or cyclophosphamide is not known. We investigated the administration of standard horse ATG (h-ATG) plus cyclosporine (CsA) in patients who were refractory to initial r-ATG/CsA (n?=?19) or cyclophosphamide/CsA (n?=?6) (registered at clinicaltrials.gov as NCT00944749). The primary endpoint was hematologic response at 3 months and was defined as no longer meeting the criteria for severe aplastic anemia. Of the 19 patients who received r-ATG as initial therapy, 4 (21%) achieved a hematologic response by 3 months, and of the 6 patients who received cyclophosphamide, only 1 (17%) responded by 6 months. Among the responders there were no cases of relapse, and in nonresponders 2 patients evolved to monosomy 7. The overall survival for the cohort at 3 years was 68% (95% CI, 50-91%). These results suggest that only a minority can be successfully salvaged after receiving as first therapy either r-ATG or cyclophosphamide. Although h-ATG may be utilized in the salvage setting, the overall response rate probably will be lower than when h-ATG is used as initial treatment. PMID:24415649

Scheinberg, Phillip; Townsley, Danielle; Dumitriu, Bogdan; Scheinberg, Priscila; Weinstein, Barbara; Rios, Olga; Wu, Colin O; Young, Neal S

2014-05-01

43

Globulin gene expression in embryos of maize viviparous mutants  

Energy Technology Data Exchange (ETDEWEB)

Expression of genes encoding the major Zea mays embryo globulins was examined in the maize precocious germination viviparous (vp) mutants. Comparison of globulin protein profiles of precociously germinating mutant embryos with those of normally germinating mature embryos revealed substantial differences with respect to the proteins encoded by the Glb1 gene. Analysis of Glb1 transcript levels in vp/vp embryos suggests that these mutants do not fully switch from a program of embryo maturation to one of germination. These preliminary studies indicate that the vp mutants provide an excellent system for the study of embryo maturation in maize. We also provide evidence for the positive regulation of Glb1 expression by the plant growth regulator abscisic acid.

Kriz, A.R.; Wallace, M.S.; Paiva, R. (Univ. of Illinois, Urbana (USA))

1990-02-01

44

Cutaneous Finding in Anti Thymocyte Globulin Induced Serum Sickness  

OpenAIRE

Polyclonal anti-thymocyte globulin (ATG) is used as an immunosuppressive agent in the treatment of aplastic anemia (AA). Serum sickness is a recognized side effect of ATG. We observed abnormal skin manifestation in patient with aplastic anemia who had been treated with ATG. We conclude that abnormal immune function caused by aplastic anemia and ATG and corticosteroids may aggravate the signs of serum sickness.

Mehran Karimi; Seyed Hesamedin Nabavizadeh; Reza Amin

2006-01-01

45

Sex Hormone Binding Globulin (SHBG), Estradiol And Breast Cancer  

OpenAIRE

Summary The human serum Sex Hormone-Binding Globulin (SHBG) plays an important role in breast cancer pathophysiology and risk definition, since it regulates the bioavailable fraction of circulating estradiol. We here summarize data reported over the years concerning the involvement of SHBG and SHBG polymorphisms in the definition of breast-cancer risk. We also report what is known about the direct action of SHBG in breast cancer cells, illustrating its interaction with these cells ...

2009-01-01

46

Sex Hormone-Binding Globulin and Type 2 Diabetes Mellitus  

OpenAIRE

Sex hormone-binding globulin (SHBG) has emerged as one of the multiple genetic and environmental factors that potentially contribute to the pathophysiology of Type 2 diabetes mellitus (T2DM). In addition to epidemiologic studies demonstrating a consistent relationship between decreased levels of serum SHBG and incident T2DM, recent genetic studies also reveal that transmission of specific polymorphisms in the SHBG gene influence risk of T2DM. On the molecular level, elucidation of the multipl...

Le, Trang N.; Nestler, John E.; Strauss, Jerome F.; Wickham, Edmond P.

2012-01-01

47

Diminished thyroxine-binding globulin in pubertal diabetic children.  

OpenAIRE

OBJECTIVE: To determine the effect of diabetes on thyroid hormone and thyroxine-binding globulin (TBG) concentrations during puberty. RESEARCH DESIGN AND METHODS: Total thyroxine (TT4), free thyroxine (FT4), and TBG levels of 171 thyroid microsomal antibody-negative subjects with normal thyroid-stimulating hormone (TSH) levels were measured and compared with those of nondiabetic adolescents. A random subset of 68 diabetic patients (40 boys and 28 girls) and 51 control subjects (24 boys and 27...

Connors, Mh; Dunger, Db; Chapel, H.; Jefferson, I.; Jowett, Tp; Edwards, Pr

1996-01-01

48

Acute lung injury during antithymocyte globulin therapy for aplastic anemia  

OpenAIRE

The case of a 33-year-old man with aplastic anemia who experienced recurrent episodes of hypoxemia and pulmonary infiltrates during infusions of antithymocyte globulin (ATG) is described. With the use of high-dose corticosteroids, the patient’s original episodes resolved, and were subsequently prevented before additional administrations of ATG. Rare reports of an association between ATG and acute lung injury are found in the literature, but this is the first report of successful steroid-sup...

Goligher, Ewan Christopher; Cserti-gazdewich, Christine; Balter, Meyer; Gupta, Vikas; Brandwein, Joseph E.

2009-01-01

49

Hepatitis B Immune Globulin in Liver Transplantation Prophylaxis: An Update  

OpenAIRE

Context: Liver transplantation is the best treatment option for end-stage liver disease following hepatitis B (HBV) infection. However, the high rate of recurrence of HBV infection following transplantation is a disadvantage of this option.Evidence Acquisition: Over the past 2 decades, the gold standard of prophylactic treatment for the prevention of HBV re-infection following liver transplantation has been the administration of low- to high-dose hepatitis B immune globulin (HBIg) along with ...

Payam Dindoost; Seyed Mohammad Jazayeri; Seyed Moayed Alavian

2012-01-01

50

Treatment of neonatal sepsis with intravenous immune globulin.  

OpenAIRE

BACKGROUND: Neonatal sepsis is a major cause of death and complications despite antibiotic treatment. Effective adjunctive treatments are needed. Newborn infants are relatively deficient in endogenous immunoglobulin. Meta-analyses of trials of intravenous immune globulin for suspected or proven neonatal sepsis suggest a reduced rate of death from any cause, but the trials have been small and have varied in quality. METHODS: At 113 hospitals in nine countries, we enrolled 3493 infants receivin...

Brocklehurst, P.; Farrell, B.; King, A.; Juszczak, E.; Darlow, B.; Haque, K.; Salt, A.; Stenson, B.; Tarnow-mordi, W.

2011-01-01

51

Cutaneous Finding in Anti Thymocyte Globulin Induced Serum Sickness  

Directory of Open Access Journals (Sweden)

Full Text Available Polyclonal anti-thymocyte globulin (ATG is used as an immunosuppressive agent in the treatment of aplastic anemia (AA. Serum sickness is a recognized side effect of ATG. We observed abnormal skin manifestation in patient with aplastic anemia who had been treated with ATG. We conclude that abnormal immune function caused by aplastic anemia and ATG and corticosteroids may aggravate the signs of serum sickness.

Mehran Karimi

2006-04-01

52

Worsening Bradycardia Following Antithymocyte Globulin Treatment of Severe Aplastic Anemia  

OpenAIRE

Immunosuppressive regimens, which include antithymocyte globulin (ATG), are widely used for the treatment of severe aplastic anemia (SAA). However, bradycardia has been reported only as a rare side effect of ATG therapy in the manufacturer's product information and, in rare cases, in the adult literature. We present an adolescent with SAA and preexisting bradycardia who underwent immunosuppression therapy with ATG, methylprednisolone, and tacrolimus and developed profound sinus bradycardia wi...

Godown, Justin; Deal, Allison M.; Riley, Kathy; Bailliard, Frederique; Blatt, Julie

2011-01-01

53

Simplified method for measuring sex-hormone binding globulin  

International Nuclear Information System (INIS)

We describe a simple, rapid method for measurement of sex-hormone binding globulin. Serial dilutions of pregnancy serum are prepared in serum from males that has been pre-treated by heating to 60 degrees C for 1 h to destroy endogenous binding globulin, which is then determined by a long-used technique to yield a set of ''standards.'' In the assay itself, a fixed amount of [3H]-labeled and unlabeled dihydrotestosterone is incubated with standard or unknown, and the bound fraction precipitated with saturated ammonium sulfate. A plot of percent of the steroid bound vs standard dilution yields a sigmoid curve, from which the results in unknowns can be read by simple extrapolation. Within-assay CVs for pools of serum from men, women, and women in late pregnancy were 6.56, 9.59, and 8.4%, respectively. Between-assay CVs for the same pools were 8.05, 9.5, and 11.5%, respectively. The correlation between results obtained by this method and those of the older technique was 0.95 for samples from non-pregnant subjects and 0.73 for those from pregnant women. Our procedure is simpler and faster than previous methods and accurately measures the differences in the globulin in sera from men, women, and pregnant women. Forty to 50 samples can be assayed in a working day

54

Simplified method for measuring sex-hormone binding globulin  

Energy Technology Data Exchange (ETDEWEB)

We describe a simple, rapid method for measurement of sex-hormone binding globulin. Serial dilutions of pregnancy serum are prepared in serum from males that has been pre-treated by heating to 60 degrees C for 1 h to destroy endogenous binding globulin, which is then determined by a long-used technique to yield a set of ''standards.'' In the assay itself, a fixed amount of (/sup 3/H)-labeled and unlabeled dihydrotestosterone is incubated with standard or unknown, and the bound fraction precipitated with saturated ammonium sulfate. A plot of percent of the steroid bound vs standard dilution yields a sigmoid curve, from which the results in unknowns can be read by simple extrapolation. Within-assay CVs for pools of serum from men, women, and women in late pregnancy were 6.56, 9.59, and 8.4%, respectively. Between-assay CVs for the same pools were 8.05, 9.5, and 11.5%, respectively. The correlation between results obtained by this method and those of the older technique was 0.95 for samples from non-pregnant subjects and 0.73 for those from pregnant women. Our procedure is simpler and faster than previous methods and accurately measures the differences in the globulin in sera from men, women, and pregnant women. Forty to 50 samples can be assayed in a working day.

Fattah, D.I.; Chard, T.

1981-07-01

55

Extraction, isolation, and characterization of globulin proteins from Lupinus albus.  

Science.gov (United States)

Lupin has recently been added to the list of allergens requiring mandatory advisory labeling on foodstuffs sold in the European Union, and since December 2008, all products containing even trace amounts of lupin must be labeled correctly. Lupin globulins consist of two major globulins called ?-conglutin (11S and "legumin-like") and ?-conglutin (7S and "vicilin-like") and another additional two globulins, ?-conglutin and ?-conglutin, which are present in lower amounts. We report on a methodology to facilitate the extraction of each of these proteins using centrifugation and isolation by anion-exchange chromatography followed by size-exclusion chromatography. The isolated subunits were characterized using reducing and non-reducing polyacrylamide gel electrophoresis, western blotting, and peptide mass fingerprinting, all of which revealed that the individual protein subunits are highly pure and can be used as immunogens for the production of antibodies specific for each of the conglutin fractions, as well as standards, and the extraction protocol can be used for the selective extraction of each of the subunits from foodstuffs, thus facilitating a highly accurate determination of the lupin concentration. Furthermore, the subunits can be used to elucidate information regarding the toxicity of each of the subunits, by looking at their interaction with the IgE antibodies found in the serum of individuals allergic to lupin, providing critical information for the definition of the requirements of analytical assays for the detection of lupin in foodstuffs. PMID:21332201

Nadal, Pedro; Canela, Nuria; Katakis, Ioanis; O'Sullivan, Ciara K

2011-03-23

56

The effect of 60Co gamma radiation on human serum gamma-globulin  

International Nuclear Information System (INIS)

The effect of 60Co gamma radiation on human serum gamma-globulin was studied in vitro experiments. Solutions of 0.5 percent gamma-globulin were exposed to 0.2, 0.5, 1.0 and 1.9 Mrad doses 60Co gamma irradiation. Experiments showed that electrophoretic mobility of serum gamma-globulin decreased after gamma irradiation. No significant change in gamma-globulin UV absorption spectrum was observed at 0.2, 0.5, 1.0 and 1.9 Mrad doses. Gamma-globulin becomes progressively less soluble in water as the radiation doses is increased. Radiation induced transformation into insoluble gamma-globulin agregates and scission products. (author)

57

Vitamin D binding globulin levels and affinity in various clinical conditions.  

OpenAIRE

The approximate association constants of the plasma vitamin D binding globulin (Gc-globulin) for 25-hydroxycholecalciferol (25(OH)D3) and the plasma 25(OH)D3 binding capacities were measured in samples from 123 patients with a variety of disorders. No gross differences in binding affinities were observed between different groups of patients and controls. Many patients, however, had moderately reduced, and several had grossly reduced, plasma binding capacities. The changes in Gc-globulin relat...

Brown, I.; Sood, A.; Carter, N. D.

1980-01-01

58

Gc globulin as a diagnostic and prognostic marker in horses : A Ph.d. project  

DEFF Research Database (Denmark)

Group specific (Gc) globulin also known as vitamin D-binding protein is part of the extracellular actin-scavenging system that removes actin from the circulation. Actin is an intracellular structural protein, which is released to blood in patients with tissue injury and cell death. Circulating actin forms filaments, which cause microthrombi and endothelial injury. These effects of circulating actin are extremely harmful, and high levels of free actin are potentially lethal. Gc-globulin binds to actin and removes it from the circulation via the reticuloendothelial system. Plasma concentrations of Gc-globulin decrease after conditions causing tissue injury and cell death, for example physical trauma, sepsis, experimentally induced endotoxemia or liver failure. In humans, decreases in Gc-globulin levels are observed within 60 minutes after trauma. Studies in humans and laboratory rodents have shown that very low concentration of plasma Gc-globulin are related to an increased risk of developing shock and lethal complications of trauma. Gc-globulin is thus a prognostic marker in intensive care medicine. It has been suggested that treatment with Gc-globulin to patients with severe tissue injury can prevent development of shock and thereby increase survival chances. The in vivo toxicity of Gc-globulin infusion is currently being investigated in horses and other species. Gc-globulin has been demonstrated in horse plasma and its structure closely resembles that of human Gc-globulin. Gc-globulin concentrations in horses under clinical conditions have never previously been investigated. The Ph.D. project focuses on Gc-globulin as a prognostic marker in horses with acute abdominal pain.

Pihl, Tina Holberg

59

Subcellular localization of the 2S globulin narbonin in seeds of Vicia narbonensis.  

Science.gov (United States)

Narbonin is a 2S protein from the globulin fraction of narbon bean (Vicia narbonensis L.) cotyledons. Its amino acid composition and the pattern of its regulated accumulation in developing seeds led to the suggestion that narbonin could be a storage protein. Therefore, it was expected to be present in protein bodies of the storage tissue cells. Comparison of the cDNA-derived amino acid sequence with a directly determined partial N-terminal sequence revealed that the primary translation product of narbonin mRNA lacks a transient N-terminal signal peptide (V.H. Nong et al., 1995, Plant Mol Biol 28: 61 - 72). Narbonin polypeptides that had been synthesized in a cell-free translation system supplemented with dog pancreas microsomes were not protected against degradation by posttranslationally added proteases (protease protection assay). In accordance with the lack of a signal peptide this indicates that the polypeptide was not cotranslationally sequestered into the microsomes. The protein-body fraction that had been isolated from mature narbon bean cotyledons by a non-aqueous gradient centrifugation procedure was free of narbonin; this was found in the soluble cell fraction. In electron micrographs, narbonin could be localized in the cytoplasm using the immuno gold-labelling technique. Previously, it had already been shown that narbonin is too slowly degraded during narbon bean germination to act as a storage protein. From all these results it has to be concluded that narbonin is a cytoplasmic protein which does not belong to the storage proteins in the restricted sense. Other possible functions are discussed. PMID:9299790

Steffens, P; Van, H N; Hillmer, S; Saalbach, I; Müntz, K

1997-09-01

60

Interference of tolerance to human gamma globulin by synthetic polynucleotides  

Energy Technology Data Exchange (ETDEWEB)

A complex of polyadenylic-polyuridylic acids effectively inhibits the in vivo production of immunologic tolerance to human gamma globulin in mice. Moreover, this effect can be obtained only when the polynucleotide complex is given within 4 hr after antigen administration. Reconstitution of irradiated mice with combinations of T and B cells originating from tolerant or previously untreated mice demonstrates that poly A:U is responsible for the adjuvant effect observed. Poly A:U exerts its adjuvant effect primarily upon T cells, while B cells remain essentially uninfluenced by the polynucleotides. (auth)

Rey, O.A.; Azar, M.M.

1975-07-01

61

Endocrine assessment of impotence--pitfalls of measuring serum testosterone without sex-hormone-binding globulin.  

OpenAIRE

The pitfalls of measuring only total serum testosterone are illustrated by a 52 year old man whose hyperprolactinaemia was associated with normal total serum testosterone but a raised sex-hormone-binding globulin, giving a low free testosterone. Prolactin suppression with bromocriptine normalized sex-hormone-binding globulin and free testosterone, and restored potency and energy after 30 years of impotence and tiredness.

Hardy, K. J.; Seckl, J. R.

1994-01-01

62

Potential drug interaction between rho(d) immune globulin and live virus vaccine.  

Science.gov (United States)

Women often receive Rho(D) immune globulin as well as a live virus vaccine in the immediate postpartum period. The immune globulin product has the potential to interfere with appropriate immune response to the vaccine. Here we describe our approach to identifying and following up on this often overlooked potential drug interaction. DOI: 10.1111/1751-486X.12165. PMID:25495973

Holmes, Amy; Wright, Debra

2014-12-01

63

Comparison of horse and rabbit antithymocyte globulin in immunosuppressive therapy for refractory cytopenia of childhood  

Science.gov (United States)

Refractory cytopenia of childhood is the most common subtype of myelodysplastic syndrome in children. In this study, we compared the outcome of immunosuppressive therapy using horse antithymocyte globulin (n=46) with that using rabbit antithymocyte globulin (n=49) in 95 patients with refractory cytopenia of childhood and hypocellular bone marrow. The response rate at 6 months was 74% for horse antithymocyte globulin and 53% for rabbit antithymocyte globulin (P=0.04). The inferior response in the rabbit antithymocyte globulin group resulted in lower 4-year transplantation-free (69% versus 46%; P=0.003) and failure-free (58% versus 48%; P=0.04) survival rates in this group compared with those in the horse antithymocyte globulin group. However, because of successful second-line hematopoietic stem cell transplantation, overall survival was comparable between groups (91% versus 85%; P=ns). The cumulative incidence of relapse (15% versus 9%; P=ns) and clonal evolution (12% versus 4%; P=ns) at 4 years was comparable between groups. Our results suggest that the outcome of immunosuppressive therapy with rabbit antithymocyte globulin is inferior to that of horse antithymocyte globulin. Although immunosuppressive therapy is an effective therapy in selected patients with refractory cytopenia of childhood, the long-term risk of relapse or clonal evolution remains. (ClinicalTrial.gov identifiers: NCT00662090) PMID:24162791

Yoshimi, Ayami; van den Heuvel-Eibrink, Marry M.; Baumann, Irith; Schwarz, Stephan; Simonitsch-Klupp, Ingrid; de Paepe, Pascale; Campr, Vit; Kerndrup, Gitte Birk; O’Sullivan, Maureen; Devito, Rita; Leguit, Roos; Hernandez, Miguel; Dworzak, Michael; de Moerloose, Barbara; Starý, Jan; Hasle, Henrik; Smith, Owen P.; Zecca, Marco; Catala, Albert; Schmugge, Markus; Locatelli, Franco; Führer, Monika; Fischer, Alexandra; Guderle, Anne; Nöllke, Peter; Strahm, Brigitte; Niemeyer, Charlotte M.

2014-01-01

64

Hepatitis B immune serum globulin and standard gamma globulin in prevention of hepatitis B infection among hospital staff: a preliminary report.  

Science.gov (United States)

In May 1973 a controlled double-blind clinical trail with prophylactic injects of hepatitis B immune serum globulin (antibody titer by passive hemagglutination 1:355,000) and standard gamma globulin (1:100) was started in Sahlgren's Hospital, Göteborg, Sweden. The annual attack rate of clinical hepatitis B in the three departments studied had been 5 to 8 per cent during recent years. A total of 118 members of the hospital staff were prophylactically treated while 125 staff members were unwilling to participate and received no prophylactic treatment. During the first 20 months of study nine cases of clinical hepatitis B with jaundice occurred within the untreated group (7.2 per cent) while three cases (2.5 per cent) were observed in prophylactically treated individuals. After decoding it was found that 60 individuals had received specific hepatitis B immune serum globulin while 58 had received standard gamma globulin. Two of the three clinical cases of hepatitis B occurred within the standard gamma globulin group. Both groups included two individuals with transient antigenemia only and the standard gamma globulin group also included four individuals with antibody seroconversion. PMID:66871

Iwarson, S; Kjellman, H; Ahlmén, J; Ljunggren, C; Eriksson, E; Selander, D; Hermodsson, S

1975-01-01

65

Sex hormone binding globulin in the rat olfactory system.  

Science.gov (United States)

Ovarian steroids are known to act on the olfactory system. Their mode of action, however, is mostly unclear to date since nuclear receptors are lacking in sensory neurons. Here we used immunocytochemistry and RT-PCR to study expression and distribution of sex hormone binding globulin (SHBG) in the rat olfactory system. Single sensory cells in the olfactory mucosa and their projections in the olfactory bulb showed specific SHBG immunostaining as determined by double immunofluorescence with olfactory marker protein OMP. Larger groups of SHBG stained sensory cells occurred in the vomeronasal organ (VNO). A portion of the olfactory glomeruli in the accessory olfactory bulb showed large networks of SHBG positive nerve fibres. Some of the mitral cells showed SHBG immune fluorescence. RT-PCR revealed SHBG encoding mRNA in the olfactory mucosa, in the VNO and in the olfactory bulbs indicating intrinsic expression of the binding globulin. The VNO and its related projections within the limbic system are known to be sensitive to gonadal steroid hormones. We conclude that SHBG may be of functional importance for rapid effects of olfactory steroids on limbic functions including the control of reproductive behaviours through pheromones. PMID:24681170

Ploss, V; Gebhart, V M; Dölz, W; Jirikowski, G F

2014-05-01

66

Possible Association between Dysfunction of Vitamin D Binding Protein (GC Globulin) and Migraine Attacks  

Science.gov (United States)

To identify the genetic causality of migraine and acute, severe melalgia, we performed a linkage analysis and exome sequencing in a family with four affected individuals. We identified a variant (R21L) in exon 2 of the GC globulin gene, which is involved in the transportation of vitamin D metabolites and acts as a chemotaxic factor; this variant was co-segregated within the family. To investigate the relationship between GC globulin and melalgia, we investigated the cytokine levels in serum samples from the patients and control subjects using a cytokine antibody array. GC globulin can bind to both MCP-1 and RANTES in human serum but has a higher affinity to MCP-1. In cell culture systems, MCP-1 was able to bind to overexpressed wild-type GC globulin but not to the GC globulin variant, and the GC globulin binding affinity to MCP-1 was significantly lower in sera from the patients than in sera from control subjects. A higher concentration of MCP-1 was also observed in sera from the patients. Thus, the dysfunctional GC globulin affected cytokine release, especially the release of MCP-1, and MCP-1 might play important roles in melalgia and migraine. PMID:25147936

Nagata, Eiichiro; Fujii, Natsuko; Hosomichi, Kazuyoshi; Mitsunaga, Shigeki; Suzuki, Yoichi; Mashimo, Yoichi; Tsukamoto, Hideo; Satoh, Tadayuki; Osawa, Motoki; Inoue, Ituro; Hata, Akira; Takizawa, Shunya

2014-01-01

67

Effects of Hyaluronic Acid and ?–Globulin Concentrations on the Frictional Response of Human Osteoarthritic Articular Cartilage  

Science.gov (United States)

Synovial fluid plays an important role in lubricating synovial joints. Its main constituents are hyaluronic acid (HA) and ?–globulin, acting as boundary lubricants for articular cartilage. The aim of the study was to demonstrate the concentration-dependent effect of HA and ?–globulin on the boundary-lubricating ability of human osteoarthritis (OA) cartilage. Normal, early and advance stage articular cartilage samples were obtained from human femoral heads and in presence of either HA or ?–globulin, cartilage frictional coefficient (µ) was measured by atomic force microscopy (AFM). In advanced stage OA, the cartilage superficial layer was observed to be completely removed and the damaged cartilage surface showed a higher µ value (?0.409) than the normal cartilage surface (?0.119) in PBS. Adsorbed HA and ?–globulin molecules significantly improved the frictional behavior of advanced OA cartilage, while they were ineffective for normal and early OA cartilage. In advanced-stage OA, the concentration-dependent frictional response of articular cartilage was observed with ?–globulin, but not with HA. Our result suggested that HA and ?–globulin may play a significant role in improving frictional behavior of advanced OA cartilage. During early-stage OA, though HA and ?–globulin had no effect on improving frictional behavior of cartilage, however, they might contribute to disease modifying effects of synovial fluid as observed in clinical settings. PMID:25426992

Son, Kyeong-Min; Thompson, Mark S.; Park, Sungchan; Chang, Jun-Dong; Nam, Ju-Suk; Park, Seonghun; Lee, Sang-Soo

2014-01-01

68

Infant botulism and indications for administration of botulism immune globulin.  

Science.gov (United States)

Infant botulism is caused by the ingestion of Clostridium botulinum spores and leads to a life-threatening descending motor weakness and flaccid paralysis in infant children. This disease presents with symptoms such as constipation, weakness, and hypotonia and can lead to respiratory failure. Botulism immune globulin (BIG) was created to treat this deadly disease and functions by neutralizing all systemically circulating botulism toxins. It is indicated in children with clinically diagnosed infant botulism, before diagnostic confirmation, and has been shown to lead to a significant reduction in intensive care unit and hospital stay for these patients. This review article discusses the epidemiology, clinical presentation, history of BIG, and indications for administration of BIG. PMID:24488164

Pifko, Elysha; Price, Amanda; Sterner, Sarah

2014-02-01

69

Homologous radioimmunoassay for guinea pig corticosteroid-binding globulin  

Energy Technology Data Exchange (ETDEWEB)

A rapid, specific, and sensitive (requiring only 20 fmole of antigen equivalent to 0.007) l of serum) radioimmunoassay (RIA) was developed for the measurement of guinea pig corticosteroid-binding globulin (CBG). CBG was purified to homogeneity from guinea pig serum by affinity chromatography and used for immunization, as the standard and as the radiolabeled trace in the RIA. The antiserum to CBG was raised in rabbits. It was judged specific by immunoelectrophoresis and by comparison of RIA values with steroid-binding assay profiles obtained on serum separated on the basis of size and ion-exchange properties. The results of the radioimmunoassays agree with those of a steroid-binding assay run on identical samples. The sensitivity of the assay allows detection of CBG in serial serum samples, other biologic fluids such as milk, and cell culture supernatants.

Hsu, B.R.S.; Kato, E.A.; Raymoure, W.J.; Kuhn, R.W.

1987-07-01

70

Diffusion and dynamics of ?-globulin in crowded aqueous solutions.  

Science.gov (United States)

Dynamics in protein solutions is essential for both protein function and cellular processes. The hierarchical complexity of global protein diffusion, side-chain diffusion, and microscopic motions of chemical groups renders a complete understanding challenging. We present results from quasi-elastic neutron scattering on protein solutions of ?-globulin over a wide range of volume fractions. Translational and rotational diffusion can be self-consistently separated from internal motions. The global diffusion is consistent with predictions for effective spheres even though the branched molecular shape differs considerably from a colloidal sphere. The internal motions are characterized both geometrically and dynamically, suggesting a picture of methyl rotations and restricted diffusion of side chains. We show that the advent of new neutron spectrometers allows the study of current questions including the coupling of intracellular dynamics and protein function. PMID:24871685

Grimaldo, Marco; Roosen-Runge, Felix; Zhang, Fajun; Seydel, Tilo; Schreiber, Frank

2014-06-26

71

Homologous radioimmunoassay for guinea pig corticosteroid-binding globulin  

International Nuclear Information System (INIS)

A rapid, specific, and sensitive (requiring only 20 fmole of antigen equivalent to 0.007) ?l of serum) radioimmunoassay (RIA) was developed for the measurement of guinea pig corticosteroid-binding globulin (CBG). CBG was purified to homogeneity from guinea pig serum by affinity chromatography and used for immunization, as the standard and as the radiolabeled trace in the RIA. The antiserum to CBG was raised in rabbits. It was judged specific by immunoelectrophoresis and by comparison of RIA values with steroid-binding assay profiles obtained on serum separated on the basis of size and ion-exchange properties. The results of the radioimmunoassays agree with those of a steroid-binding assay run on identical samples. The sensitivity of the assay allows detection of CBG in serial serum samples, other biologic fluids such as milk, and cell culture supernatants

72

Androgen regulated expression of the alpha 2u-globulin gene in pancreatic hepatocytes of rat  

OpenAIRE

Under a copper-deficient regimen, pancreatic cells in the adult rat can be found to undergo differentiation into hepatocytes. Pancreatic hepatocytes induced in male and female rats were examined for the expression of the androgen-inducible hepatic protein, alpha 2u- globulin. Alpha 2u-Globulin protein was demonstrable by immunoperoxidase method in all the pancreatic hepatocytes of male rats. Northern blot analysis confirmed the presence of 1.3 kb alpha 2u- globulin mRNA transcript in the panc...

1990-01-01

73

Labeling of human immune gamma globulin with sup(99m)Tc  

International Nuclear Information System (INIS)

Human immune serum gamma globulin and rabbit anti-Stap. aureus antibody have been successfully labeled with sup(99m)Tc at pH 7.4 with an average binding efficiency of 86 and 82%, respectively. The labeled proteins behave similarly to unlabeled gamma-globulin fraction in the normal human serum as demonstrated by protein electrophoresis. The biological half-time of sup(99m)Tc-gamma-globulin in dog has been determined to be 54 min for the fast component and 14.7 hr for a slower component. Immunological assays demonstrate no significant change in antibody activity after labeling process. (author)

74

SIFAT FUNGSIONAL PRODUK INTERAKSI FRAKSI GLOBULIN 7S KOMAK (Dolichos lablab DAN GUM XANTAN [Functional Properties of the Interaction Product Between Globulin of 7S Fraction of Lablab Bean (Dolichos lablab with Xantan Gum  

Directory of Open Access Journals (Sweden)

Full Text Available Lablab bean (Dolichos lablab seeds is a potential source of protein globulin.The bean’s protein content is 20.86 %, and the amount of globulin was more than 60% from the total protein, having major fractions of 7S and 11S. The objectives of this research were to explore the 7S globulin fractions, to study interaction between 7S globulin fractions with xanthan gum, and to observe the functional properties of the product of the interaction. The research was conducted in 2 steps. The first step was to fractionate the 7S fractions from globulin. The second steps was to interact 7S globulin fraction with xanthan gum. The yield of these interaction were examined for its physicochemical and functional properties. The results showed that the 7S globulin fractions could be interacted by xanthan gum at pH 7. The interacted product of globulin 7S fraction 10 % with xanthan gum 0,75 % had good functional properties than globulin 7S fraction, such as oil holding capacity, foaming capacity, and emulsion activity. Water holding capacity could not be detected because the yield became soluble. However,the foaming and emulsifying stability were still lower than those of soybean protein isolates. The research concluded that xanthan gum could be used to improve the physicochemical and functional properties of globulin 7S fraction.

Sukamto1*

2009-12-01

75

Studies on the role of glycosylation for human corticosteroid-binding globulin: Comparison with that for thyroxine-binding globulin  

Energy Technology Data Exchange (ETDEWEB)

The role of glycosylation on the secretion and the stability of human corticosteroid binding globulin (CBG) was studied. Cells of the human hepatoma line were labeled by ({sup 35}S)methionine in presence of or absence of tunicamycin (TM). Media or cells were harvested at 0, 3, 6, and 20 h after the addition of excess unlabeled methionine. Media and cell lysates were incubated with anti-CBG serum and immune complexes were precipitated with Staphylococcus aureus protein A (Pansorbin). Immunoprecipitates were analyzed by fluorography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoprecipitation of T4-binding globulin (TBG) was also carried out with anti-TBG serum. Fluorographic analysis revealed three forms of CBG: CBG1, a glycosylated, mature, and secretory form with apparent mol wt of 70 K; CBG2, a glycosylated precursor which due to incomplete carbohydrate processing has an apparent mol wt of 54 K; and CBG3, a nonglycosylated form consisting of the 40 K core protein. In absence of TM, CBG1 was observed in media and CBG2 was detected in cell lysates. The proportion of CBG1 increased during the chase, whereas that of CBG2 decreased, indicating that CBG was secreted after processing of the oligosaccharides on CBG2. In presence of TM, CBG3 was found both in media and cell lysates. The sum of CBG3 in the medium and the cell lysate decreased during the chase, whereas that of CBG1 and CBG2 remained unchanged. Similar to CBG, TBG1 (mature form, 60 K) and TBG2 (partially processed glycosylated form, 54 K) were observed in media and cell lysates, respectively, in absence of TM. However, TBG3 (nonglycosylated, 44 K) was not detected in medium. These results indicate that glycosylation is not a key factor for the secretion of CBG but is important for its stability. On the other hand the glycosylation is indispensable for the secretion of TBG.

Murata, Y.; Sueda, K.; Seo, H.; Matsui, N. (Nagoya Univ. (Japan))

1989-09-01

76

Studies on the role of glycosylation for human corticosteroid-binding globulin: Comparison with that for thyroxine-binding globulin  

International Nuclear Information System (INIS)

The role of glycosylation on the secretion and the stability of human corticosteroid binding globulin (CBG) was studied. Cells of the human hepatoma line were labeled by [35S]methionine in presence of or absence of tunicamycin (TM). Media or cells were harvested at 0, 3, 6, and 20 h after the addition of excess unlabeled methionine. Media and cell lysates were incubated with anti-CBG serum and immune complexes were precipitated with Staphylococcus aureus protein A (Pansorbin). Immunoprecipitates were analyzed by fluorography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoprecipitation of T4-binding globulin (TBG) was also carried out with anti-TBG serum. Fluorographic analysis revealed three forms of CBG: CBG1, a glycosylated, mature, and secretory form with apparent mol wt of 70 K; CBG2, a glycosylated precursor which due to incomplete carbohydrate processing has an apparent mol wt of 54 K; and CBG3, a nonglycosylated form consisting of the 40 K core protein. In absence of TM, CBG1 was observed in media and CBG2 was detected in cell lysates. The proportion of CBG1 increased during the chase, whereas that of CBG2 decreased, indicating that CBG was secreted after processing of the oligosaccharides on CBG2. In presence of TM, CBG3 was found both in media and cell lysates. The sum of CBG3 in the medium and the cell lysate decreased during the chase, whereas that of CBG1 and CBG2 remained unchanged. Similar to CBG, TBG1 (mature form, 60 ed. Similar to CBG, TBG1 (mature form, 60 K) and TBG2 (partially processed glycosylated form, 54 K) were observed in media and cell lysates, respectively, in absence of TM. However, TBG3 (nonglycosylated, 44 K) was not detected in medium. These results indicate that glycosylation is not a key factor for the secretion of CBG but is important for its stability. On the other hand the glycosylation is indispensable for the secretion of TBG

77

Allosteric Modulation of Hormone Release from Thyroxine and Corticosteroid-binding Globulins*  

OpenAIRE

The release of hormones from thyroxine-binding globulin (TBG) and corticosteroid-binding globulin (CBG) is regulated by movement of the reactive center loop in and out of the ?-sheet A of the molecule. To investigate how these changes are transmitted to the hormone-binding site, we developed a sensitive assay using a synthesized thyroxine fluorophore and solved the crystal structures of reactive loop cleaved TBG together with its complexes with thyroxine, the thyroxine fluorophores, furosemi...

Qi, Xiaoqiang; Loiseau, Franc?ois; Chan, Wee Lee; Yan, Yahui; Wei, Zhenquan; Milroy, Lech-gustav; Myers, Rebecca M.; Ley, Steven V.; Read, Randy J.; Carrell, Robin W.; Zhou, Aiwu

2011-01-01

78

Cortisol-binding globulin and meat quality in five European lines of pigs  

OpenAIRE

The gene (Cbg) encoding cortisol-binding globulin (CBG) has been proposed as a candidate gene to explain genetic variation in cortisol secretion and carcass composition in pigs. The objective of this study was to evaluate the association between CBG and pork quality in 5 European breeding lines, Pietrain, Large White (LW), and Landrace purebred lines, a Duroc synthetic line, and a Meishan (MS) x LW advanced intercross. Cortisol-binding globulin maximum binding capacity (CBG-Bmax) was twice as...

Geverink, N. A.; Foury, A.; Plastow, G. S.; Gil, L.; Gispert, M.; Horto?s, M.; Font I Furnols, M.; Gort, G.; Moisan, M. P.; Morme?de, P.

2006-01-01

79

Effectiveness of Intravenous Gamma-Globulin Treatment in Guillain-Barre Syndrome  

OpenAIRE

Tehran, have been studied for effectiveness of intravenous Gamma-Globulin treatment. The diagnosis was based upon patients history, physical signs and cerebrospinal fluid changes, in addition, electromyographic and nerve velocity conduction studies in some of them. Patients were divided into 3 groups: Control group (Without any pharmacologic treatment), short-term corticosteroid administration, and intravenous Gamma-Globulin therapy (Groups consisted of 16, 8 and 9 patients respectively). The...

Mohammadi, M.

1999-01-01

80

Pre-exposure prophylaxis with immune serum globulin for prevention of viral hepatitis in army recruits.  

OpenAIRE

A controlled trial with pre-exposure immune serum globulin was undertaken in Israel Defence Forces (IDF) recruits, an indigenous population living in a hyperendemic area for hepatitis A. The objective was to examine whether the prevailing IDF policy of postexposure administration of immune serum globulin prophylaxis should be modified to further reduce the incidence of infectious hepatitis in the IDF. Altogether 23 447 recruits were systematically allocated on their first day of service into ...

Kark, J. D.

1982-01-01

81

The effect of immune globulin on the response to trivalent oral poliovirus and yellow fever vaccinations  

OpenAIRE

To assess whether immune globulin may be administered concurrently with trivalent oral poliovirus vaccine (OPV) or yellow fever vaccine, antibody responses were studied in Peace Corps volunteers embarking for overseas duty in 1978. Of 200 volunteers who received OPV, 192 (96%) had pre-existing neutralizing antibody to at least 2 poliovirus types; of 160 yellow fever vaccinees, 24 (15%) had pre-existing 17D yellow fever antibody. Each volunteer received 5 ml of immune globulin, 0-7 days before...

Kaplan, Jonathan E.; Nelson, David B.; Schonberger, Lawrence B.; Hatch, Milford H.; Monath, Thomas P.; Lazuick, John S.; Calisher, Charles H.; Rosa, Franz W.

1984-01-01

82

Globulin-platelet model predicts minimal fibrosis and cirrhosis in chronic hepatitis B virus infected patients  

OpenAIRE

AIM: To establish a simple model consisting of the routine laboratory variables to predict both minimal fibrosis and cirrhosis in chronic hepatitis B virus (HBV)-infected patients. METHODS: We retrospectively investigated 114 chronic HBV-infected patients who underwent liver biopsy in two different hospitals. Thirteen parameters were analyzed by step-wise regression analysis and correlation analysis. A new fibrosis index [globulin/platelet (GP) model] was developed, including globulin (GLOB) ...

Xu-Dong Liu; Jian-Lin Wu; Jian Liang; Tao Zhang; Qing-Shou Sheng

2012-01-01

83

Influence of immunization on serum ?-globulin levels of calves following whole-body X irradiation  

International Nuclear Information System (INIS)

Calves aged 2.5 to 4 months were whole-body X irradiated with mean lethal doses between 1.2 and 1.7 Gy. The effect of different immunization procedures on the irradiation-induced reaction of the serum gamma globulin levels was studied. Immunization 14 and 21 days before irradiation resulted in obvious stimulation gamma globulin production. After parenteral antigen administration the nearly 2 weeks lasting increase of the gamma globulin level rose in the irradiated animals but declined in the sham-irradiated calves. After a lethal dosis of 1.7 Gy there was a decrease of the gamma globulins 3 weeks post irradiation, at the climax of the radiation syndrome. When 1.5 Gy were used the increase of the gamma globulin concentration was observed also after oral administration of the antigen. The response of the irradiated animals in the secondary reaction of the antibody production was most lear after boosting with homologous bacteria. The stimulating effect of the irradiation on the serum globulin levels after immunization prior to irradiation has been attributed to the reaction of the immunoglobulin-producing system to the release of tissue proteins and antigens, respectively

84

Study of the influence of homologous serum globulin preparations on the intestinal automicroflora in irradiated mice  

Energy Technology Data Exchange (ETDEWEB)

In spite of considerable experience of practical use of serum globulin preparations, their effect on automicroflora wasn't studied. The favorable effect of therapeutic injection of homologous serum globulin preparations on automicroflora of small and large intestine of mices was established for the model of acute radiation sickness caused by /sup 60/Co irradiation with 700 R dose. The effect of injecting two types of globulin preparations was studied: ones prepared of blood of intact and hemostimulated mices (to increase the content of normal antitissue antibodies in the serum). Besides the general globulin fraction isolated by ammonium sulfate precipitation a study was made on the effect of purified IgG and IgM preparations. Threefold subcutaneous or intraperitoneal globulin in ection of 1 ..mu..g dose in a mice prevented after 2, 24, 48 h after irradiation the development of bacteriosis, typical for radiation injury - decreased accumulation of putrefactive bacteria and reduced the suppression of lactobacilli content. Globulin preparations and fractions of hemostimulated mice serum, enriched by normal antitissue antibodies are the most effective ones.

Pinegin, B.V.; Klemparskaya, N.N.; Mal' tsev, V.N.; Korshunov, G.A.; Shal' nova, G.A.; Kuz' mina, T.D.

1984-09-01

85

Safety Pharmacology, Toxicology and Pharmacokinetic Assessment of Human Gc Globulin (Vitamin D Binding Protein)  

DEFF Research Database (Denmark)

Gc globulin is an important protein of the plasma actin-scavenger system. As such, it has been shown to bind free actin and prevent hypercoagulation and shock in patients with massive actin release resulting from severe tissue injuries. Treatment of such patients with Gc globulin could therefore potentially be life-saving. This article presents pre-clinical toxicology experiments conducted on purified plasma-derived human Gc globulin. The Gc globulin formulation was shown to be stable for at least 4 years with full retention of actin-binding capacity. In vitro studies did not reveal activation of the kallikrein system or the complement system and cellular studies showed no toxic effects on a variety of human cell lines. In vivo studies showed no acute toxic effects in mice, rats or guinea pigs upon intravenous infusion. A 14-day local tolerance study in rabbits showed no adverse effects, and 14-day toxicity studies in rats and horses did not show any unwanted reactions. In a 14-day toxicology study in beagle dogs, formation of antibodies was seen and in the end of the study period, three out of four dogs showed clinical immunological reactions, which could be ascribed to the formation of antibodies. The half-life, T, for human Gc globulin was 12 hr in rats, 16 hr in horses and 30 hr in dogs. The safety profile of plasma-derived Gc globulin is concluded to be consistent to that required for use in man.

Pihl, Tina Holberg; JØrgensen, Charlotte Svaerke

2010-01-01

86

Gene duplication and an accelerated evolutionary rate in 11S globulin genes are associated with higher protein synthesis in dicots as compared to monocots  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Seed storage proteins are a major source of dietary protein, and the content of such proteins determines both the quantity and quality of crop yield. Significantly, examination of the protein content in the seeds of crop plants shows a distinct difference between monocots and dicots. Thus, it is expected that there are different evolutionary patterns in the genes underlying protein synthesis in the seeds of these two groups of plants. Results Gene duplication, evolutionary rate and positive selection of a major gene family of seed storage proteins (the 11S globulin genes, were compared in dicots and monocots. The results, obtained from five species in each group, show more gene duplications, a higher evolutionary rate and positive selections of this gene family in dicots, which are rich in 11S globulins, but not in the monocots. Conclusion Our findings provide evidence to support the suggestion that gene duplication and an accelerated evolutionary rate may be associated with higher protein synthesis in dicots as compared to monocots.

Li Chun

2012-01-01

87

SIFAT FUNGSIONAL PRODUK INTERAKSI FRAKSI GLOBULIN 7S KOMAK (Dolichos lablab) DAN GUM XANTAN [Functional Properties of the Interaction Product Between Globulin of 7S Fraction of Lablab Bean (Dolichos lablab) with Xantan Gum  

OpenAIRE

Lablab bean (Dolichos lablab) seeds is a potential source of protein globulin.The bean’s protein content is 20.86 %, and the amount of globulin was more than 60% from the total protein, having major fractions of 7S and 11S. The objectives of this research were to explore the 7S globulin fractions, to study interaction between 7S globulin fractions with xanthan gum, and to observe the functional properties of the product of the interaction. The research was conducted in 2 steps. The first s...

Sukamto; Aulanni’am; Sudiyono

2009-01-01

88

Hepatitis B immune globulin preparations and use in liver transplantation.  

Science.gov (United States)

Hepatitis B immune globulin remains a central component of prophylaxis in HBV-infected patients undergoing liver transplantation. HBIG monotherapy given at a high dosage and indefinitely can prevent recurrence in 65% to 80% of patients. Because treatment failures occur and combination therapy using HBIG plus a nucleoside analog is more uniformly effective, the current standard of care is combination HBIG plus a nucleoside analog. These combination protocols have reduced the rate of virologic breakthrough to 10% or less. Several questions remain. The optimal dose and duration of HBIG use is unclear. Moreover, the development of resistance to lamivudine (and other nucleoside analogs) before transplantation increases the risk for virologic breakthrough post-transplantation. For patients with pre-transplant evidence of active HBV replication caused by the presence of nucleoside analog resistance, HBIG may be the main or only form of prophylaxis. For these patients, HBIG doses sufficient to maintain anti-HBs titers comparable to the days of HBIG monotherapy seem warranted. New HBIG formulations have made anti-HBs therapy more safe and tolerable to patients but the cost of the drug remains significant. The cost factor is particularly important in developing countries or countries with more limited resources for management of liver transplant recipients. Thus, there remains a need to develop and test new forms of anti-HBs therapy, so that effective but less expensive forms of immunoprophylaxis can be made available. PMID:14509525

Terrault, Norah A; Vyas, Girish

2003-08-01

89

A solid-phase radioimmunoassay for human corticosteroid binding globulin  

International Nuclear Information System (INIS)

A radioimmunoassay (RIA) for human corticosteroid binding globulin (CBG) has been developed using 125I-labelled CBG and a monospecific solid-phase CBG-antiserum (CBG-Ab-cellulose). The specificity of the RIA was confirmed for serial dilutions of male, female and pregnancy sera, as well as pure CGB standards. The mean +- S.D.recovery (99 +- 8%) verified the accuracy of the method, and a good correlation existed between serum CBG cortisol binding capacity measurements and CBG concentrations measured by RIA. Intra- and interassay precisions at low to high serum CBG concentrations were < 5% and < 9% respectively. The mean +- S.D. serum CBG concentrations measured were: 21.8 +- 4.6 in boys, 20.0 +- 4.2 in girls, 20.7 +- 2.7 in men, 20.5 +- 2.9 in women and 47.1 +- 10.5 in pregnant women. The sensitivity of the standard curve was 1.0 ng CBG/assay tube, but this could be increased to 0.2 ng/assay tube by reducing the amount of CBG-Ab-cellulose used. The RIA is suitable for both clinical and research purposes, and will aid the identification of abnormal forms of CBG and facilitate studies of the regulation of CBG production in vitro. (author)

90

Radioimmunoassay of human sex hormone binding globulin: improved radioiodination procedure  

Energy Technology Data Exchange (ETDEWEB)

Sex hormone binding globulin (SHBG), purified by affinity chromatography from retroplacental blood plasma, was reacted with 3-(p-hydroxyphenyl) propionic acid N-hydroxysuccinimidyl ester (PHPPS, Bolton-Hunter reagent). The derivative of SHBG obtained (parahydroxyphenylpropionyl-SHBG; PHPP-SHBG) was stable and could, in contrast to underived SHBG, be efficiently SVI-iodinated with a lactoperoxidase technique. The PHPP-SHBG labelled with SVI had good antiserum binding and stability properties and was used for radioimmunoassay (RIA) of SHBG in serum. The RIA requires a total incubation time of 3 h. It has been standardized with purified SHBG and has a sensitivity of 5 g/l, giving a lowest detectable concentration in the routine procedure of about 0.2 mg/l. Variation within and between assay was 4.1% and 7.2%, respectively, for samples with values within the normal range. Values obtained by this RIA procedure correlate well with those obtained by a dihydrotestosterone binding method and by an electroimmunoassay technique. The mean serum concentration of SHBG in healthy, regularly menstruating women (n=42) was 3.7 +- 1.0 mg/l and in healthy men (n=100) 2.0 +- 0.9 mg/l. (Author).

Fernlund, P.; Larsson, I.; Gershagen, S.; Rannevik, G.

1985-09-01

91

Hepatitis B Immune Globulin in Liver Transplantation Prophylaxis: An Update  

Directory of Open Access Journals (Sweden)

Full Text Available Context: Liver transplantation is the best treatment option for end-stage liver disease following hepatitis B (HBV infection. However, the high rate of recurrence of HBV infection following transplantation is a disadvantage of this option.Evidence Acquisition: Over the past 2 decades, the gold standard of prophylactic treatment for the prevention of HBV re-infection following liver transplantation has been the administration of low- to high-dose hepatitis B immune globulin (HBIg along with an antiviral agent to induce passive immunity.Results: The effectiveness of HBIg in preventing the recurrence of HBV depends on the dosage, route of administration, and duration of HBIg treatment, and the viremic status at the time of transplantation. There is currently no consensus on a standardized recommendation for therapeutic options that include HBIg administration.Conclusion: This review attempts to summarize the available data on the feasibility of such options. Most recent studies support the use of long-term combination therapy of HBIg and antiviral NAs (especially new agents.

Payam Dindoost

2012-03-01

92

Solid-phase radioimmunoassay for human corticosteroid binding globulin  

Energy Technology Data Exchange (ETDEWEB)

A radioimmunoassay (RIA) for human corticosteroid binding globulin (CBG) has been developed using /sup 125/I-labelled CBG and a monospecific solid-phase CBG-antiserum (CBG-Ab-cellulose). The specificity of the RIA was confirmed for serial dilutions of male, female and pregnancy sera, as well as pure CGB standards. The mean +- S.D.recovery (99 +- 8%) verified the accuracy of the method, and a good correlation existed between serum CBG cortisol binding capacity measurements and CBG concentrations measured by RIA. Intra- and interassay precisions at low to high serum CBG concentrations were < 5% and < 9% respectively. The mean +- S.D. serum CBG concentrations measured were: 21.8 +- 4.6 in boys, 20.0 +- 4.2 in girls, 20.7 +- 2.7 in men, 20.5 +- 2.9 in women and 47.1 +- 10.5 in pregnant women. The sensitivity of the standard curve was 1.0 ng CBG/assay tube, but this could be increased to 0.2 ng/assay tube by reducing the amount of CBG-Ab-cellulose used. The RIA is suitable for both clinical and research purposes, and will aid the identification of abnormal forms of CBG and facilitate studies of the regulation of CBG production in vitro.

Robinson, P.A.; Langley, M.S.; Hammond, G.L. (Univ. of Manchester Medical School, Hope Hospital, Salford (UK))

1985-02-01

93

Immunomodulatory treatment of myelodysplastic syndromes: antithymocyte globulin, cyclosporine, and alemtuzumab  

Science.gov (United States)

It is now well accepted that a subgroup of patients with myelodysplastic syndromes (MDS) can recover from pancytopenia following immunosuppressive treatment (IST). For many years immunosuppression with antilymphocyte antibodies has been a standard treatment approach for patients with severe aplastic anemia. The initial concept of using immunosuppression to treat pancytopenic patients with MDS was based on the premise that MDS might share with severe aplastic anemia (SAA) an autoimmune basis for the bone marrow failure common to both conditions. The idea was supported by reports of favorable outcomes in occasional cases of MDS treated with antithymocyte globulin (ATG). Today, various forms of IST have been successfully used to restore hematopoiesis in MDS in many centers worldwide. In this review we outline the rationale for use of IST in MDS, and describe studies which help to define the patients with MDS likely to respond to IST. We summarize 18 published clinical trials using IST for MDS and discuss how these studies have helped to define the MDS subgroups likely to respond to treatment, the nature and durability of the response, the impact of IST on long-term outcome and the best treatment approach. PMID:23079060

Parikh, A; Olnes, MJ; Barrett, AJ

2014-01-01

94

Open controlled therapeutic trial of intravenous immune globulin in relapsing-remitting multiple sclerosis.  

Science.gov (United States)

Ten patients with relapsing-remitting multiple sclerosis were treated with intravenous immune globulin, 0.4 g/kg per day for 5 consecutive days, and then with additional booster doses of immune globulin of 0.4 g/kg, once every 2 months, for the next 12 months. Ten untreated patients with relapsing-remitting multiple sclerosis who were matched with the study patients for age, disease duration, and number of attacks per year served as controls. Immune globulin treatment was well tolerated, with no side effects. The exacerbation rate decreased from 3.7 +/- 1.2 exacerbations per year before treatment to 1.0 +/- 0.7 exacerbations per year during the treatment in the immune globulin-treated patients, while it remained unaltered in the controls. The posttreatment Kurtzke Expanded Disability Status Scale score decreased from a mean of 4.45 to 4.15, whereas in controls it increased from 3.55 to 3.75. The results suggest that immune globulin suppresses the ongoing pathologic process in multiple sclerosis and may be a promising treatment to prevent disease exacerbations. PMID:1449400

Achiron, A; Pras, E; Gilad, R; Ziv, I; Mandel, M; Gordon, C R; Noy, S; Sarova-Pinhas, I; Melamed, E

1992-12-01

95

Effect of variations in globulin concentrations on serum radioimmunoassay results, as exemplified by choriogonadotropin  

International Nuclear Information System (INIS)

Variations in serum protein concentrations can affect results of radioimmunoassay of human choriogonadotropin involving either polyethylene glycol or double-antibody methods of separation. Relative to the maximal binding of choriogonadotropin in the presence of normal (30 g/L) globulin concentrations, globulin concentrations of 10 to 60 g/L were associated with maximal binding ranging from 84.9 to 115.6% in the polyethylene glycol system, and from 109.9 to 92.5% in the double-antibody system. This effect was quantitatively much the same throughout the standard curve in the polyethylene glycol system, but diminished with the higher standard concentrations in the double-antibody system. In assays involving polyethylene glycol separation, an increase in globulin was associated with a linear increase in nonspecific binding, but no detectable effect of globulin on nonspecific binding was observed in the double-antibody system. Determination and compensation for nonspecific binding of individual samples (rather than for mean assay nonspecific binding) eliminated the globulin effect in the polyethylene glycol system but not in the double-antibody system

96

Effect of variations in globulin concentrations on serum radioimmunoassay results, as exemplified by choriogonadotropin  

Energy Technology Data Exchange (ETDEWEB)

Variations in serum protein concentrations can affect results of radioimmunoassay of human choriogonadotropin involving either polyethylene glycol or double-antibody methods of separation. Relative to the maximal binding of choriogonadotropin in the presence of normal (30 g/L) globulin concentrations, globulin concentrations of 10 to 60 g/L were associated with maximal binding ranging from 84.9 to 115.6% in the polyethylene glycol system, and from 109.9 to 92.5% in the double-antibody system. This effect was quantitatively much the same throughout the standard curve in the polyethylene glycol system, but diminished with the higher standard concentrations in the double-antibody system. In assays involving polyethylene glycol separation, an increase in globulin was associated with a linear increase in nonspecific binding, but no detectable effect of globulin on nonspecific binding was observed in the double-antibody system. Determination and compensation for nonspecific binding of individual samples (rather than for mean assay nonspecific binding) eliminated the globulin effect in the polyethylene glycol system but not in the double-antibody system.

Jawad, M.J.; Wilson, E.A.

1981-12-01

97

Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen.  

Science.gov (United States)

The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane. These models have been particularly useful in defining the participation of effector leukocytes in severe glomerular disease. In these models, injury typically occurs in two phases. In the initial, heterologous phase, injury is mediated by the globulin bound within the glomerulus acting as an antibody. The later, autologous phase of injury is mediated by the host's adaptive immunity to the heterologous globulin now functioning as a planted foreign antigen within glomeruli. As autologous phase injury is driven by immunity to sheep globulin, assessment of antigen-specific systemic immunity to sheep globulin is critical when using this model. Curr. Protoc. Immunol. 106:15.26.1-15.26.20. © 2014 by John Wiley & Sons, Inc. PMID:25081909

Odobasic, Dragana; Ghali, Joanna R; O'Sullivan, Kim M; Holdsworth, Stephen R; Kitching, A Richard

2014-01-01

98

Adsorption of immunogamma globulin onto various synthetic calcium hydroxyapatite particles.  

Science.gov (United States)

This paper presents data on adsorption of immunogamma globulin (IgG) onto synthetic rodlike calcium hydroxyapatite particles (CaHaps) with various particle lengths and calcium/phosphate (Ca/P) atomic ratios ranging from 1.54 to 1.65 and compares the obtained results to those of acidic (bovine serum albumin, BSA), neutral (myoglobin, MGB), and basic (lysozyme, LSZ) proteins reported before. The effect of electrolyte concentration on IgG adsorption was also examined. The initial rate of IgG adsorption was similar to that of BSA and was slower than that of MGB and LSZ. This fact was interpreted by the difference in the structural stability and molecular weight of these proteins. The isotherms of IgG adsorption onto the CaHap particles were of pseudo-Langmuir type. The saturated amount of adsorbed IgG values (nsIgG) for the particles with mean particle length less than 70 nm decreased with increasing Ca/P ratio. The adsorption behavior of IgG molecules was very similar to that of basic LSZ, though IgG has zero net charge. The nsIgG value was increased with increased mean particle length of CaHaps; the relationship was less significant than that for BSA but similar to those for MGB and LSZ. The similar adsorption behavior of IgG and LSZ suggested that the Fab parts of IgG molecules preferentially adsorb onto CaHap to provide the reversed Y-shaped conformation of IgG. The change of the adsorption mode of IgG molecules from the reversed Y-shaped conformation to side-on by "spreading" the Fc part of IgG molecules onto the particle surface over a longer adsorption time was suggested. The nsIgG value was increased with increasing electrolyte concentration by screening the intra- and intermolecular electrostatic interactions of proteins. PMID:15082375

Kandori, Kazuhiko; Miyagawa, Kaori; Ishikawa, Tatsuo

2004-05-15

99

Polyclonal antithymocyte globulin and cardiovascular disease in kidney transplant recipients.  

Science.gov (United States)

T-lymphocyte activation may contribute to atherosclerosis, the prevalence of which is increased in transplant patients. However, the cardiovascular consequences of polyclonal antithymocyte globulin (ATG)-induced immune modifications, which include alterations in T-cell subsets, are unknown. We conducted a retrospective single-center study to assess whether ATG associates with an increased incidence of atherosclerotic events (CVEs) in kidney transplant patients. Propensity score analysis was performed to address potential confounding by indication. We also tested whether ATG use induces a proatherogenic immune status. Sixty-nine (12.2%) CVEs occurred during follow-up (87±31 months). The cumulative incidence of CVEs was higher in ATG-treated patients (14.7% versus 8.2%; P=0.03). Cox regression analysis revealed that ATG use was an independent risk factor for CVEs (hazard ratio [HR], 2.36; 95% confidence interval [95% CI], 1.35 to 4.13; P=0.003). Results obtained in the propensity score match analysis recapitulated those obtained from the overall cohort (HR, 2.09; 95% CI, 1.11 to 3.98; P=0.02). Late-stage differentiated CD8(+) T cells increased 1 year after transplantation only in ATG-treated patients. More generally, ATG associated with features of immune activation. These modifications increased markedly in patients exposed to cytomegalovirus (CMV). Subanalyses suggest that the effect of ATG on CVEs is restricted to CMV-exposed patients. However, CMV infection associated significantly with CVEs only in ATG-treated patients (HR, 2.07; 95% CI, 1.16 to 3.70; P=0.01). In conclusion, ATG associated with both immune activation and post-transplant CVEs in this cohort. Further studies should precisely determine whether ATG-induced immune activation is the causal link between ATG and CVEs. PMID:24511120

Ducloux, Didier; Courivaud, Cécile; Bamoulid, Jamal; Crepin, Thomas; Chalopin, Jean-Marc; Tiberghien, Pierre; Saas, Philippe

2014-06-01

100

Naturally Occurring Mutations of Human Corticosteroid-binding Globulin.  

Science.gov (United States)

Context: Corticosteroid-binding globulin (CBG) is encoded by SERPINA6. It is the major plasma binding protein of glucocorticoids and regulates plasma cortisol levels and bioavailability in humans. Several proteases target CBG and disrupt its steroid-binding properties. To date, most genetic deficiencies that alter plasma CBG levels or function have been identified in patients presenting with a variety of clinical conditions. Objective: To test thirty-two previously uncharacterized non-synonymous, single nucleotide polymorphisms (SNPs) in SERPINA6 for their ability to alter CBG production and/or function. Design: Human CBG mutants were produced in Chinese hamster ovary cells for ELISA, cortisol-binding activity measurements and Western blotting, as well as assays of their protease sensitivities. Results: Eight naturally occurring CBG mutants with abnormal production and/or function were identified. Cortisol-binding affinity was markedly reduced for CBG H14Q and CBG H89Y, moderately decreased for CBG I279F, and undetectable for CBG R260L. By contrast, CBG H14R exhibited a decreased cortisol-binding capacity. Comparison of CBG levels in cell extracts and media by Western blotting revealed that CBG I48N and CBG P246Q have secretion defects. Two mutants (CBG I179V and CBG I279F) displayed reduced rates of cortisol-binding activity loss after exposure to three different proteases (neutrophil elastase, chymotrypsin, and LasB produced by Pseudomonas aeruginosa). Conclusion: Our data provide insight into how specific residues affect CBG secretion or function, and illustrate the need to consider the various naturally occurring human CBG mutations in clinical evaluations of diseases associated with abnormalities in cortisol levels or activity. PMID:25322275

Simard, Marc; Hill, Lesley A; Lewis, John G; Hammond, Geoffrey L

2014-10-16

101

Energy storage  

International Nuclear Information System (INIS)

This chapter discusses the role that energy storage may have on the energy future of the US. The topics discussed in the chapter include historical aspects of energy storage, thermal energy storage including sensible heat storage, latent heat storage, thermochemical heat storage, and seasonal heat storage, electricity storage including batteries, pumped hydroelectric storage, compressed air energy storage, and superconducting magnetic energy storage, and production and combustion of hydrogen as an energy storage option

102

Effect of Co-60 irradiation on hyperimmune antimeningococcus globulins-gamma  

International Nuclear Information System (INIS)

Globulins-gamma from voluntary blood donors immunized with the Cuban BC antimeningococcus vaccine is now being used in our country for the treatment of the meningococcus disease. This study of the effect of Co-60 irradiation on antimeningococcus globulins-gamma was carried out to try to eliminate the inconvenience shown by the traditionally used sterilization procedures (losses in the filter and persistence of viral contamination). globulins-gamma was obtained by ethanol fractionation and was irradiated at a different dose in solution with different stabilizers and it was also lyophilized. Results of the chemical controls carried out lead to the conclusion that it is possible to use radiosterilization on this product in a lyophilized form. The preservation of bactericidal activity, even after the highest irradiation doses, confirms the above mentioned. 13 refs

103

Interaction of serum sex steroid-binding globulin with cell membranes of human decidual tissue  

Energy Technology Data Exchange (ETDEWEB)

The interaction of the sex steroid-binding globulin (SBG) of human blood with plasma membranes of cells from human decidual tissue - the target tissue of estradiol - was studied. It was shown that SBG in complex with estradiol is capable of interacting specifically with these membranes. The dissociation (K/sub dis/) of this interaction is equal to (3.5 +/- 2.0) 10/sup -12/ M. The interaction of the SBG-estradiol complex with the membranes is characterized by high selectivity: such blood serum globulins as albumin, orosomucoid, transferrin, transcortin, and thyroxine-binding globulin do not compete with SBG for its binding sites on the membranes. The SBG-testosterone complex and SBG without steroid are also incapable of interacting with the membranes.

Avvakumov, G.V.; Survilo, L.I.; Strel' chenok, O.A.

1986-01-20

104

Effectiveness of Intravenous Gamma-Globulin Treatment in Guillain-Barre Syndrome  

Directory of Open Access Journals (Sweden)

Full Text Available Tehran, have been studied for effectiveness of intravenous Gamma-Globulin treatment. The diagnosis was based upon patients history, physical signs and cerebrospinal fluid changes, in addition, electromyographic and nerve velocity conduction studies in some of them. Patients were divided into 3 groups: Control group (Without any pharmacologic treatment, short-term corticosteroid administration, and intravenous Gamma-Globulin therapy (Groups consisted of 16, 8 and 9 patients respectively. The results were evaluated according to short or long term response to treatment. There was no significant difference in short term response to treatment in all three groups, the long term response being significantly better in the group treated with intravenous Gamma-Globulin than in the other 2 groups.

M Mohammadi

1999-07-01

105

Release of flavonoids from lupin globulin proteins during digestion in a model system.  

Science.gov (United States)

Lupin seed globulin proteins form complexes with flavonoids, predominantly apigenin C-glycosides. Enzymes typical for the gastrointestinal tract were used to hydrolyze lupin seed globulins. Release of native flavonoids as a result of the proteolysis reaction was observed. Different analytical methods such as size exclusion chromatography, HPLC-MS, and fluorescence spectroscopy (steady-state fluorescence, fluorescence anisotropy, fluorescence lifetimes) were used for a detailed characterization of this phenomenon. Flavonoids liberated from lupin globulin proteins as a result of pancreatin-catalyzed digestion were bound by ?-conglutin resistant to this enzyme. Two possible mechanisms of this interaction may be suggested: hydrogen bonding between oligosaccharide chains of glycoproteins and the sugar moieties of the flavonoid glycosides or electrostatic attraction between positively charged ?-conglutin and flavonoids partially ionized at pH 7.5. PMID:22264085

Czubinski, Jaroslaw; Dwiecki, Krzysztof; Siger, Aleksander; Kachlicki, Piotr; Neunert, Grazyna; Lampart-Szczapa, Eleonora; Nogala-Kalucka, Malgorzata

2012-02-22

106

ALTERATIONS IN TOTAL PROTEIN CONCENTRATION, SERUM PROTEIN FRACTIONS AND ALBUMIN/GLOBULIN RATIO IN HEALTHY RABBITS  

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Full Text Available This study assessed the effect of oral administration of Aloe vera and was to evaluate total serum protein, albumin and globulin concentrations as well as albumin / globulin (A / G ratio. Twenty rabbits weighing 1000 – 1800 g were divided into 2 groups. Each group consisted of ten animals. One served as control and other group served as experimental group. Results show that animals after 07, 15 and 30 days dosing of Aloe vera showed highly significant decrease in total protein and globulin and highly significant decrease in Albumin after 15 and 30 days of dosing of Aloe vera in comparison to control animals group. It is concluded that the long-term use of Aloe vera may cause hypoglobinemia and hypoalbuminemia at 30 days of dosing and it could be due to the liver diseases, evidence of hepatotoxicity induced Aloe vera also reported in previous studies.

Nuzhat Sultana

2013-08-01

107

Comparison of horse and rabbit anti-thymocyte globulin in immunosuppressive therapy for refractory cytopenia of childhood  

DEFF Research Database (Denmark)

Refractory cytopenia of childhood is the most common subtype of myelodysplastic syndrome in children. In this paper, we compared the outcome of immunosuppressive therapy using horse anti-thymocyte globulin (n = 46) with that using rabbit anti-thymocyte globulin (n = 49) in 95 patients with refractory cytopenia of childhood and hypocellular bone marrow. The response rate at 6 months was 74% for horse anti-thymocyte globulin and 53% for rabbit anti-thymocyte globulin (p = 0.04). The inferior response in the rabbit anti-thymocyte globulin group resulted in lower 4-year-transplantation-free (69% vs. 46%; p = 0.003) and failure-free (58% vs. 48%; p = 0.04) survival in this group compared with those in the horse anti-thymocyte globulin group. However, because of successful second-line hematopoietic stem cell transplantation, overall survival was comparable between groups (91% vs. 85%, p = ns). The cumulative incidence of relapse (15% vs. 9%, p = ns) and clonal evolution (12% vs. 4%, p = ns) at 4 years was comparable between groups. Our results suggest that the outcome of immunosuppressive therapy with rabbit anti-thymocyte globulin is inferior to that of horse anti-thymocyte globulin. Although immunosuppressive therapy is an effective therapy in selected patients with refractory cytopenia of childhood, the long-term risk of relapse or clonal evolution remains. (ClinicalTrial.gov identifiers: NCT00662090).

Yoshimi, Ayami; van den Heuvel-Eibrink, Marry M

2014-01-01

108

Studies on a possible using of penicillin and specific globulin for treatment of Siberia ulcer infection in irradiated animals  

International Nuclear Information System (INIS)

The efficiency of anti-anthracic globulin and penicillin for treating infectious anthrax was compared in experiments on 160 guinea pigs and 400 white mice irradiated with sub-lethal doses of cobalt-60 gamma rays. It was found that penicillin retained its effectiveness in the irradiated animals whereas anti-anthracic globulin lost much of its therapeutic efficiency. (auth.)

109

Proteomic analysis of albumin and globulin fractions of pea (Pisum sativum L. seeds  

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Full Text Available Background. Proteomic analysis is emerging as a highly useful tool in food research, including studies of food allergies. Two-dimensional gel electrophoresis involving isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis is the most effective method of separating hundreds or even thousands of proteins. In this study, albumin and globulin tractions of pea seeds cv. Ramrod were subjected to proteomic analysis. Selected potentially alergenic proteins were identified based on their molecular weights and isoelectric points. Material and methods. Pea seeds (Pisum sativum L. cv. Ramrod harvested over a period of two years (Plant Breeding Station in Piaski-Szelejewo were used in the experiment. The isolated albumins, globulins and legumin and vicilin fractions of globulins were separated by two-dimensional gel electrophoresis. Proteomic images were analysed in the ImageMaster 2D Platinum program with the use of algorithms from the Melanie application. The relative content, isoelectric points and molecular weights were computed for all identified proteins. Electrophoregrams were analysed by matching spot positions from three independent replications. Results. The proteomes of albumins, globulins and legumin and vicilin fractions of globulins produced up to several hundred spots (proteins. Spots most characteristic of a given fraction were identified by computer analysis and spot matching. The albumin proteome accumulated spots of relatively high intensity over a broad range of pi values of -4.2-8.1 in 3 molecular weight (MW ranges: I - high molecular-weight albumins with MW of -50-110 kDa, II - average molecular-weight albumins with MW of -20-35 kDa, and III - low molecular-weight albumins with MW of -13-17 kDa. 2D gel electrophoregrams revealed the presence of 81 characteristic spots, including 24 characteristic of legumin and 14 - of vicilin. Conclusions. Two-dimensional gel electrophoresis proved to be a useful tool for identifying pea proteins. Patterns of spots with similar isoelectric points and different molecular weights or spots with different isoelectric points and similar molecular weights play an important role in proteome analysis. The regions characteristic of albumin, globulin and legumin and vicilin fractions of globulin with typical MW and pi values were identified as the results of performed 2D electrophoretic separations of pea proteins. 2D gel electrophoresis of albumins and the vicilin fraction of globulins revealed the presence of 4 and 2 spots, respectively, representing potentially allergenic proteins. They probably corresponded to vicilin fragments synthesized during post-translational modification of the analysed protein.

Jerzy Dziuba

2014-06-01

110

Marked elevation and cyclic variation of corticosteroid-binding globulin: an inherited abnormality?  

Science.gov (United States)

Unexplained high serum corticosteroid-binding globulin (CBG) concentrations [mean values, 78.8 and 55.7 mg/L; normal women, 38.8 +/- 3.8 (+/- SD) mg/L] were found repeatedly in two apparently healthy sisters who were not pregnant or taking exogenous estrogens. One had substantial variations in serum CBG and sex hormone-binding globulin concentrations during the menstrual cycle, which paralleled the normal cyclic changes in serum estradiol. The other woman was postmenopausal and had a high serum CBG concentration despite of low serum estradiol levels. We conclude those women have an inherited abnormality in CBG production. PMID:2493032

Coolens, J L; Heyns, W

1989-02-01

111

State of microquantities of yttrium-91 in aqueous protein-containing solutions. Solutions of globulin  

Energy Technology Data Exchange (ETDEWEB)

The extraction of microquantities of yttrium-91 and serum ..gamma..-globulin into foam from solutions of 0.15 M NaCl was studied as a function of the pH. An investigation of the kinetics of the process showed that in acid medium (pH = 4.7) there is an extraction of the complex of /sup 91/Y with globulin, while in weakly alkaline medium (pH = 8.1) there is a parallel flotation of colloidal forms of the radionuclide. The processes were described by the equations of a first-order reaction, for which the rate constants were determined.

Mikshevich, N.V.; Egorov, Yu.V.; Semenov, D.I.; Puzako, V.D.

1975-01-01

112

Adrenal steroids in the brain: role of the intrinsic expression of corticosteroid-binding globulin (CBG) in the stress response.  

Science.gov (United States)

The complex interaction between hypothalamus, pituitary and adrenal glands is a key component of the neuroendocrine stress response. The major stress hormones--glucocorticoids--have both central and peripheral effects. Among the factors regulating their availability to target tissues are levels of corticosteroid-binding globulin, as the major transport protein for glucocorticoids in systemic circulation. Our recent findings demonstrated expression of corticosteroid-binding globulin in various brain regions and in different cell populations (neurons and glial cells). We showed at the cellular level the presence of corticosteroid-binding globulin in the human hypothalamus, where it was co-localized with the classical neurohypophyseal neurohormones--vasopressin and oxytocin. For the first time we demonstrated in mouse that the same gene encodes brain and liver corticosteroid-binding globulin. The full-length sequencing of hypothalamic corticosteroid-binding globulin revealed a full homology with liver corticosteroid-binding globulin cDNA. Thus, we confirmed that corticosteroid-binding globulin mRNA is produced locally within various cerebral regions and thus not transported from blood. However, the amounts of mRNA encoding corticosteroid-binding globulin are in liver about 200 times higher than in brain. The wide distribution of corticosteroid-binding globulin, distinct from the localization of glucocorticoid receptors, observed in our comparative study in rodents, led us to propose two possibilities: (1) corticosteroid-binding globulin is made in certain neurons to deliver glucocorticoids into the cell and within the cell in the absence of cytoplasmic glucocorticoid receptors or (2) is internalized into neurons specifically to deliver glucocorticoids to classical glucocorticoid receptors. Brain corticosteroid-binding globulin may be involved in the response to changing systemic glucocorticoid levels either additionally to known nuclear and membrane corticosteroid receptors or in glucocorticoid responsive brain regions devoid of these receptors. Clearly the multiple locations of corticosteroid-binding globulin within the central nervous system of humans and rodents imply multiple functional properties in normal and/or pathological conditions, which are yet to be determined. Most likely, the importance of brain corticosteroid-binding globulin exceeds the function of a mere steroid transporter. PMID:24246737

Sivukhina, Elena V; Jirikowski, Gustav F

2014-03-01

113

Proteomic Analysis of Albumins and Globulins from Wheat Variety Chinese Spring and Its Fine Deletion Line 3BS-8  

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Full Text Available The relationship between chromosome deletion in wheat and protein expression were investigated using Chinese Spring and fine deletion line 3BS-8. Through 2-DE (2-D electrophoresis analysis, no differentially expressed proteins (DEPs were found in leaf samples; however, 47 DEPs showed at least two-fold abundance variation (p < 0.05 in matured wheat grains and 21 spots were identified by tandem MALDI-TOF/TOF-MS. Among the identified spots, four were cultivar-specific, including three (spots B15, B16, and B21 in Chinese Spring and one in 3BS-8 (spot B10. Among variety-different DEPs between Chinese Spring and 3BS-8, most spots showed a higher express profile in CS; only four spots showed up-regulated expression tendency in 3BS-8. An interesting observation was that more than half of the identified protein spots were involved in storage proteins, of which 11 spots were identified as globulins. According to these results, we can presume that the encoded genes of protein spots B15, B16, and B21 were located on the chromosome segment deleted in 3BS-8.

Yue-Ming Yan

2012-10-01

114

A systematic review of intravenous gamma globulin for therapy of acute myocarditis  

OpenAIRE

Abstract Background Intravenous gamma globulin (IVGG) is commonly used in the management of acute myocarditis. The objective of this study was to systematically review the literature evaluating this practice. Methods We conducted a comprehensive search (electronic databases, trials registries, conference proceedings, reference lists, contact with authors) to identify studies evaluating the use of IVGG in adults and children with a clinical or histologically prov...

Crumley Ellen; Hartling Lisa; Robinson Joan L; Vandermeer Ben; Klassen Terry P

2005-01-01

115

Gene amplification as a cause for inherited thyroxine-binding globulin excess?  

Energy Technology Data Exchange (ETDEWEB)

This editorial reviews the past research regarding thyroxine-binding globulins (TBGs) and their role in hereditary diseases involving plasma concentrations of protein bound iodine. The genetic basis for TBG excess revolves around the expression and mutations of the gene for TBG. 23 refs.

Robbins, J. [National Institutes of Health, Bethesda, MD (United States)

1995-12-01

116

Reproductive endocrinology: functional effects of sex hormone-binding globulin variants.  

Science.gov (United States)

A new study has found eight single nucleotide polymorphisms in sex hormone-binding globulin that functionally affect its affinity for androgens or estrogens and other biochemical properties. This finding adds to growing concern about the ‘one size fits all’ approach in formulas to calculate free or bioavailable concentrations of estradiol and testosterone. PMID:25048036

Laurent, Michaël R; Vanderschueren, Dirk

2014-09-01

117

Sex hormone-binding globulin levels predict insulin sensitivity, disposition index, and cardiovascular risk during puberty  

DEFF Research Database (Denmark)

Early puberty is associated with increased risk of subsequent cardiovascular disease. Low sex hormone-binding globulin (SHBG) levels are a feature of early puberty and of conditions associated with increased cardiovascular risk. The aim of the present study was to evaluate SHBG as a predictor of glucose metabolism and metabolic risk during puberty.

SØrensen, Kaspar; Aksglaede, Lise

2009-01-01

118

Is the sex hormone binding globulin related to preeclampsia independent of insulin resistance  

International Nuclear Information System (INIS)

Objective: To evaluate the association between Sex Hormone Binding Globulin and preeclampsia in Iranian women considering the probable confounding effect of insulin resistance. Methods: The case-control study was conducted at the Semnan University of Medical Sciences, Iran, and comprised pregnant women who received prenatal care at Amiralmomenin Hospital in 2011. Cases represented patients admitted because of preeclampsia, while controls were randomly selected eligible pregnant women without hypertension and/or proteinuria. Fasting blood sugar and insulin were assessed for all participants as well as their blood concentration of Sex Hormone Binding Globulin. The Homeostasis Model Assessment of Insulin Resistance Score was used. The correlation between dependant and independent variables was reported by crude and adjusted odds ratio applying logistic regression models. SPSS 16.0 was used for statistical analysis. Results: Of the 100 pregnant women in the study, 45(45%) were cases. Insulin resistance was found to be significantly more frequent in the cases compared to the controls (adjusted odds ratio=2.78; 95% Confidence Interval: 1.11, 6.90; p<0.01). There was a significant reverse correlation between level of Sex Hormone Binding Globulin in blood and being a case of preeclampsia (adjusted odds ratio=0.99; 95% Confidence Interval: 0.98, 1.00; p=0.04). Conclusion: Independent of insulin resistance, every 1nmol/l increase in Sex Hormone Binding Globulin, decreases the odds of preeclampsia by 1%, notifying Sex Hormone Binding Globulin as an important biomarker about its etiology and prediction. (author)

119

Energy Storage.  

Science.gov (United States)

Described are technological considerations affecting storage of energy, particularly electrical energy. The background and present status of energy storage by batteries, water storage, compressed air storage, flywheels, magnetic storage, hydrogen storage, and thermal storage are discussed followed by a review of development trends. Included are…

Eaton, William W.

120

Determination of thyroxine-binding globulin in human serum by single radial immunodiffusion and radioimmunoassay. [/sup 125/I tracer technique  

Energy Technology Data Exchange (ETDEWEB)

Two immunochemical methods for determination of thyroxine-binding globulin in human serum were developed, in which the purified globulin and monospecific antiserum to it are used. One method, based on radial immunodiffusion, has good precision and values for analytical recovery. Reference values obtained for men were 9.8 to 17.8 mg/liter and for women 11.3 to 20.5 mg/liter. The sex-related difference was significant. The other method is based on radioimmunoassay, with use of an iodinated acylating agent for the labeling of thyroxine-binding globulin. The relative merits of the two methods are discussed.

Kagedal, B.; Kallberg, M.

1977-09-01

121

Transcortin and alpha 2u-globulin messenger RNA activities during turpentine-induced inflammation in the rat.  

Science.gov (United States)

Previously we have shown that the serum concentration of transcortin and alpha 2u-globulin markedly decreases during turpentine-induced inflammation. In the present study transcortin and alpha 2u-globulin mRNA from healthy rats and from animals with inflammation was translated in a rabbit reticulocyte lysate system. Female rats had higher levels of translatable transcortin mRNA than male animals and the level of mRNA for transcortin and alpha 2u-globulin decreased rapidly during inflammation. These results indicate that the sex difference in the serum level of transcortin and the changes in serum transcortin and alpha 2u-globulin during inflammation are mainly determined by differences in the mRNAs in the liver. PMID:2413280

Faict, D; Verhoeven, G; Mertens, B; De Moor, P

1985-09-01

122

Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes  

OpenAIRE

The classical Osborne wheat proteins fraction (albumin-globulin), as well as several polypeptides from the non-gluten protein fraction using SDS-PAGE analyses were determined in the grain of five bread (T. aestivum L.) and five durum wheat (T. durum Desf.) genotypes. In addition, the activity rate of lipoxygenase (LOX) and peroxidase (POD) enzymes implicated in the antioxidant metabolism was determined. Albumins and globulins in wheat grains were characteri...

Žili? Sla?ana; Bara? Miroljub; Peši? Mirjana; Hadži-Taškovi?-Šukalovi? Vesna; Dodiga Dejan; Mladenovi?-Drini? Snežana; Jankovi? Marijana

2011-01-01

123

Predictive value of serum actin-free Gc-globulin for complications and outcome in acute liver failure.  

Science.gov (United States)

This prospective study was designed to evaluate whether early changes in actin-free Gc-globulin levels were associated with complications and outcomes and to identify factors associated with persistent low actin-free Gc-globulin levels in acute liver failure (ALF). Thirty-two consecutive ALF patients admitted from October 2011 to December 2012 were followed up until death or complete recovery. All had serum actin-free Gc-globulin estimation at admission and at day three or expiry. Logistic regression analysis was performed to identify independent predictors of mortality. A receiver operating characteristic curve analysis was also performed. Nonsurvivors had significantly lower median actin-free Gc-globulin levels than survivors (87.32 vs 180 mg/L; P predict mortality with 92% sensitivity and 71.4% specificity. Patients with lower serum actin-free Gc-globulin levels and decreasing trend in serum actin-free Gc-globulin levels were found to have more mortality and developed more complications. Logistic regression analysis showed that serum actin-free Gc-globulin, total leucocyte count and serum creatinine at admission were independent predictors of mortality. Incorporating these variables, a score predicting mortality risk at admission was derived. The scoring system was compared to MELD score and King's College Criteria as individual predictor of mortality. Serum actin-free Gc-globulin level at presentation is predictive of outcome and can be used for risk stratification. Its persistent low-level predicts mortality and is correlated with various complications. PMID:24774007

Bagchi, A; Kumar, S; Ray, P C; Das, B C; Gumma, P K; Kar, P

2015-02-01

124

Effect of inclusion of cashew globulin (Anacardium occidentale) to a casein diet on lipid parameters in rats.  

Science.gov (United States)

The effect of inclusion of cashew globulin to a casein diet on lipid metabolism was studied in rats fed diets with two levels of cashew globulin meal. Inclusion of cashew globulin to a casein diet produced lower levels of total cholesterol, triacylglycerol and phospholipids in the serum and tissues and lower levels of serum lipoprotein cholesterol. There was decreased cholesterogenesis in the liver as evidenced by decreased activity of HMG CoA reductase and decreased release of lipoproteins into circulation. Rats fed cashew globulin along with casein also showed higher activity of LPL in the heart and adipose tissue and higher activity of LCAT. Increased hepatic diversion of cholesterol to bile acid synthesis and increased excretion of bile acids and sterols were also observed in these groups. Activity of glucose-6-phosphate dehydrogenase and malic enzyme was decreased in rats fed cashew globulin along with casein. This study demonstrates that cashew globulins included in the diet of rats are able to alter lipid metabolism which results in lower levels of lipid parameters in the serum and tissues. PMID:10890760

Prabha, S P; Rajamohan, T

1998-01-01

125

Enantioselective recognition of mandelic acid based on ?-globulin modified glassy carbon electrode.  

Science.gov (United States)

A new chiral biosensor has been fabricated by immobilizing ?-globulin on gold nanoparticles modified glassy carbon electrodes, which could recognize and detect mandelic acid (MA) enantiomers. Differential pulse voltammetry, quartz crystal microbalance, ultraviolet-visible spectroscopy, and atomic force microscopy were used to characterize the enantioselectivity. The results exhibited that ?-globulin modified electrode could enantioselectively recognize MA enantiomers, and larger response signals were obtained from R-MA. The factors influencing the performance of the resulting biosensor were investigated. The enantiomeric composition of R- and S-MA enantiomer mixtures could be determined by measuring the current responses of the sample. The developed electrodes have the advantages of simple preparation, good stability, and rapid detection. PMID:22056409

Fu, Yingzi; Chen, Qiao; Zhou, Juan; Han, Qian; Wang, Yonghua

2012-02-01

126

Radioimmunological analysis of the content of thyroid hormones, and thyroxin binding globulin in the blood  

International Nuclear Information System (INIS)

The content of general thyroxin (T4) and triiodothyronine (T3) as well as the absolute content of free thyroxin (ACT4) and thyroxin binding globulin have been studied in the blood of normal people, patients with thyrotoxicosis, hypothyrosis, obesity, diabetes mellitus and also in pregnant women by a radioimmunological method using Corning kits. It has been shown that the mean concentration of thyroxin binding globulin in patients with thyrotoxicosis is decreased. However the TBG determination cannot be used as a diagnostic test for this disease because of notable variations of individual indices. T4, T3 and ACT4 concentration in the blood was decreased and TBG concentration increased in patients with hypothyrosis. The content of thyroid hormones did not change in obesity, diabetes mellitus and in pregnant women in spite of the increased TBG concentration in the blood

127

Verification of a method for sexual hormone-binding globulin analysis and estimation of free testosterone  

OpenAIRE

Introduction: Sexual hormone-binding globulin (SHBG) is a protein that binds to androgens and oestrogens, especially testosterone. The fraction of testosterone that is not bound to SHBG is the biologically active fraction which makes its determination more relevant than determining the total amount of circulating testosterone. It is difficult to measure the plasma concentration of free testosterone; therefore calculations using the concentrations of testosterone and SHBG are used to estimate ...

Englund, Sofia

2012-01-01

128

Low Sex-Hormone Binding Globulin is Associated with the Metabolic Syndrome in Postmenopausal Women  

OpenAIRE

Although an association between the metabolic syndrome and hyperandrogenism has been suggested in women with polycystic ovarian syndrome, few studies have investigated this relationship in postmenopausal women. We measured estradiol, testosterone, and sex hormone binding globulin (SHBG) and calculated the free androgen index (FAI) in 212 postmenopausal women not using hormone therapy in the Women's Health Study. A modified ATP III definition of the metabolic syndrome (3 or more of the followi...

Weinberg, Melissa E.; Manson, Joann E.; Buring, Julie E.; Cook, Nancy R.; Seely, Ellen W.; Ridker, Paul M.; Rexrode, Kathryn M.

2006-01-01

129

Cortisol binding capacity of corticosteroid binding globulin in hyperadrenocorticoid and healthy dogs.  

Science.gov (United States)

Corticosteroid binding globulin (CBG) binding capacity and plasma cortisol concentration were determined in 27 dogs with hyperadrenocorticism and in 17 healthy control dogs. Cortisol concentrations were significantly higher in hyperadrenocorticoid dogs than in controls. CBG binding capacity did not differ between the two groups. It is concluded that excessive endogenous cortisol secretion does not induce an increase in CBG binding capacity. Consequently, the determination of CBG binding capacity provides no additional information for the diagnosis of canine Cushing's syndrome. PMID:1620969

Bamberg-Thalén, B; Nyberg, L; Fackler, L; Edqvist, L E

1992-05-01

130

Circadian and circaseptan rhythmicities in corticosteroid-binding globulin (CBG) binding activity of human milk.  

OpenAIRE

It has been demonstrated that human milk contains a corticosteroid-binding globulin (CBG, transcortin) which is very similar to that isolated from plasma. It is agreed, moreover, that the cortisol environment of the breast is important for the synthesis of major milk proteins. It was of interest, therefore, to investigate whether milk transcortin exhibits circadian variations in the binding activity as does the plasma counterpart. Twelve lactating women, aged 23-35 years, collected specimens ...

Angeli, Alberto; Bertino, Enrico; Frairia, Roberto

1982-01-01

131

Hyporesponsiveness to Glucocorticoids in Mice Genetically Deficient for the Corticosteroid Binding Globulin  

OpenAIRE

Corticosteroid binding globulin (CBG) is the carrier for glucocorticoids in plasma. The protein is believed to keep the steroids inactive and to regulate the amount of free hormone acting on target tissues (free hormone hypothesis). Here, we generated a mouse model genetically deficient for CBG to test the contribution of the carrier to glucocorticoid action and adrenocortical stress response. The absence of CBG resulted in a lack of corticosterone binding activity in serum and in an ?10-fo...

Petersen, Helle Heibroch; Andreassen, Thomas K.; Breiderhoff, Tilman; Bra?sen, Jan Hinrich; Schulz, Herbert; Gross, Volkmar; Gro?ne, Hermann-josef; Nykjaer, Anders; Willnow, Thomas E.

2006-01-01

132

N-Glycans Modulate the Function of Human Corticosteroid-Binding Globulin*  

OpenAIRE

Human corticosteroid-binding globulin (CBG), a heavily glycosylated protein containing six N-linked glycosylation sites, transports cortisol and other corticosteroids in blood circulation. Here, we investigate the biological importance of the N-glycans of CBG derived from human serum by performing a structural and functional characterization of CBG N-glycosylation. Liquid chromatography-tandem MS-based glycoproteomics and glycomics combined with exoglycosidase treatment revealed 26 complex ty...

Sumer-bayraktar, Zeynep; Kolarich, Daniel; Campbell, Matthew P.; Ali, Sinan; Packer, Nicolle H.; Thaysen-andersen, Morten

2011-01-01

133

Corticosteroid-Binding Globulin: Structure-Function Implications from Species Differences  

OpenAIRE

Corticosteroid-binding globulin (CBG) transports glucocorticoids and progesterone in the blood and thereby modulates the tissue availability of these hormones. As a member of the serine protease inhibitor (SERPIN) family, CBG displays a reactive center loop (RCL) that is targeted by proteinases. Cleavage of the RCL is thought to trigger a SERPIN-typical stressed-to-relaxed (S-to-R) transition that leads to marked structural rearrangements and a reduced steroid-binding affinity. To characteriz...

Gardill, Bernd R.; Vogl, Michael R.; Lin, Hai-yan; Hammond, Geoffrey L.; Muller, Yves A.

2012-01-01

134

Sex hormone binding globulin concentration as a prepubertal marker for hyperinsulinaemia in obesity  

OpenAIRE

BACKGROUND—Overweight children become obese adults who are prone to develop the "metabolic syndrome" and premature coronary arterial disease (CAD).?AIMS—To assess whether sex hormone binding globulin (SHBG) is a potential marker for hyperinsulinaemia/insulin resistance in prepubertal obese children.?METHODS—Twenty five obese children (body mass index (BMI) >2SD) who warranted investigation on clinical grounds were enrolled. Their insulin response to an oral glucose...

Galloway, P.; Donaldson, M.; Wallace, A.

2001-01-01

135

Anti-thymocyte Globulin plus Etanercept as Therapy for Myelodysplastic Syndromes (MDS): a Phase II Study  

OpenAIRE

Immunosuppressive therapies have proven valuable in treating patients with myelodysplastic syndromes (MDS). We evaluated the combination of equine anti-thymocyte globulin (ATGAM®) and the soluble TNF receptor etanercept (Enbrel®) in a phase II trial. Twenty-five patients with MDS (4-RA, 2-RARS, 15-RCMD, 3-RCMD-RS, 1-RAEB-1) in IPSS risk groups low (n=11) or intermediate-1 (n=14) were enrolled. All patients were platelet or red cell transfusion dependent. Nineteen patients completed therapy ...

Scott, Bart L.; Ramakrishnan, Aravind; Fosdal, Mark; Storer, Barry; Becker, Pamela; Petersdorf, Steve; Deeg, H. Joachim

2010-01-01

136

Genetic variants of sex hormone-binding globulin and their biological consequences  

OpenAIRE

Abstract Several hormonal and metabolic factors have been found to influence the production of sex hormone-binding globulin (SHBG). In addition, twin studies have suggested that genetic factors may also contribute to variation in SHBG levels. Given the clinical significance of SHBG in regulating bioavailable sex steroid hormones, a number of studies examined the potential association between polymorphisms of SHBG gene and serum SHBG levels as well as their possible contribution in ...

2009-01-01

137

Sex hormone-binding globulin gene expression in the liver: drugs and the metabolic syndrome  

OpenAIRE

Abstract Sex hormone-binding globulin (SHBG) is the main transport binding protein for sex steroid hormones in plasma and regulates their accessibility to target cells. Plasma SHBG is secreted by the liver under the control of hormones and nutritional factors. In the human hepatoma cell line (HepG2), thyroid and estrogenic hormones, and a variety of drugs including the antioestrogen tamoxifen, the phytoestrogen, genistein and mitotane (Op’DDD) increase SHBG production and SHBG ge...

2009-01-01

138

High serum thyroxine-binding globulin--an important cause of hyperthyroxinaemia.  

OpenAIRE

Four patients are described who had a significant elevation of serum thyroxine-binding globulin (TBG). Review of these patients indicated that inappropriate therapy for thyrotoxicosis had been given in three cases. A study of our laboratory records revealed that significant TBG elevation was a relatively common phenomenon which occurred in approximately 0.45% of our hospital population. Patients with this abnormality have serum thyroxine levels which are comparable to those with clinical thyr...

Leahy, B. C.; Davies, D.; Laing, I.; Walton, L.

1984-01-01

139

Ligand binding studies of human steroid-binding globulin in a biosensorsystem  

OpenAIRE

In der vorliegenden experimentellen Studie wurden zum einen mittels Gleich-gewichtsmessungen in Lösung und zum anderen mit der Oberflächenplasmon-resonanz-Biosensorik verschiedene Aminoalkyl-5a-Dihydrotestosteron-Derivate in ihren Wechselwirkung mit dem humanen Sexualhormon-bindenden Globulin (hSHBG) untersucht. Durch kinetische und thermodynamische Daten zu Assoziation und Dissoziation der Liganden gelang eine funktionelle Charakterisierung der bio-spezifischen Anbindung dieser Steroidhorm...

Schnitzbauer, Andreas

2006-01-01

140

Human sex hormone-binding globulin gene expression- multiple promoters and complex alternative splicing  

OpenAIRE

Abstract Background Human sex hormone-binding globulin (SHBG) regulates free sex steroid concentrations in plasma and modulates rapid, membrane based steroid signaling. SHBG is encoded by an eight exon-long transcript whose expression is regulated by a downstream promoter (PL). The SHBG gene was previously shown to express a second major transcript of unknown function, derived from an upstream promoter (PT), and two minor transcripts. Results ...

Rosner William; Hryb Daniel J; Nakhla Atif M; Romas Nicholas A; Xiang Zhaoying; Kahn Scott M

2009-01-01

141

Increased globulin and its association with hemorrhagic transformation in patients receiving intra-arterial thrombolysis therapy.  

Science.gov (United States)

Previous studies have identified a diverse set of predisposing factors for the occurrence of hemorrhagic transformation (HT), but the independent clinical predictors of HT after intraarterial thrombolysis have not been determined. In this retrospective study, we investigated the characteristics of patients with or without HT who had received intra-arterial thrombolysis therapy, using biochemical analysis, renal function test, routine blood test, blood lipid test, coagulation blood test, liver function test, random blood glucose test, time-window for intra-arterial thrombolysis, recanalization, National Institutes of Health Stroke Scale (NIHSS) score and systolic blood pressure before intra-arterial thrombolysis. The mortality rates were similar in the HT and non-HT groups (P = 0.944). In the single-factor analysis, patients with a higher globulin level (P <0.002), prothrombin time activity percentage (PTA; P = 0.026), and NIHSS score (P = 0.002), had a significantly increased risk of developing HT. In the multifactor logistic regression model involving globulin level, PTA, white blood cell count, and NIHSS score, the globulin level (P <0.001; OR, 1.185; 95% confidence interval [CI], 1.090-1.288), PTA (P = 0.018; OR, 1.016; 95% CI, 1.003-1.029), white blood cell count (P = 0.025; OR, 1.097; 95% CI, 1.012-1.190) and NIHSS score (P = 0.003; OR, 1.097; 95% CI, 1.031-1.166) were significantly increased in the HT group. The increase in globulin level is an independent risk factor for HT in patients receiving intra-arterial thrombolysis. The possible mechanisms may involve inflammatory cytokines, matrix metalloproteinase 9, and positive acute-phase reactants synthesized by the liver. PMID:24871645

Xing, Yingqi; Guo, Zhen-Ni; Yan, Shuo; Jin, Hang; Wang, Shouchun; Yang, Yi

2014-06-01

142

Glycosaminoglycans that bind cold-insoluble globulin in cell-substratum adhesion sites of murine fibroblasts.  

OpenAIRE

Glycosaminoglycans (GAGs) and glycoprotein-derived glycopeptide from mouse BALB/c3T3 and simian virus 40-transformed 3T3 whole cells or their adhesion sites, which are left bound to the serum-coated tissue culture substratum after detachment of cells mediated by [ethylenebis-(oxyethylenenitrilo]tetraacetic acid (EGTA), were analyzed for specific binding to Sepharose columns derivatized with cold-insoluble globulin (CIg). CIg is the serum-contained form of fibronectin and is required for the a...

Laterra, J.; Ansbacher, R.; Culp, L. A.

1980-01-01

143

Identification of sex hormone-binding globulin in the human hypothalamus  

OpenAIRE

Gonadal steroids are known to influence hypothalamic functions through both genomic and non-genomic pathways. Sex hormone-binding globulin ( SHBG) may act by a non-genomic mechanism independent of classical steroid receptors. Here we describe the immunocytochemical mapping of SHBG-containing neurons and nerve fibers in the human hypothalamus and infundibulum. Mass spectrometry and Western blot analysis were also used to characterize the biochemical characteristics of SHBG in the hypothalamus ...

Herbert, Zso?fia; Go?the, Susanne; Caldwell, Jack D.; Bernstein, Hans-gert; Melle, Christian; Eggeling, Ferdinand Von; Lewis, John; Jirikowski, Gustav F.

2005-01-01

144

Proteomic analysis of albumin and globulin fractions of pea (Pisum sativum L.) seeds  

OpenAIRE

Background. Proteomic analysis is emerging as a highly useful tool in food research, including studies of food allergies. Two-dimensional gel electrophoresis involving isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis is the most effective method of separating hundreds or even thousands of proteins. In this study, albumin and globulin tractions of pea seeds cv. Ramrod were subjected to proteomic analysis. Selected potentially alergenic proteins were identified...

Jerzy Dziuba; Iwona Szerszunowicz; Dorota Na??cz; Marta Dziuba

2014-01-01

145

Postexposure Prevention of Progressive Vaccinia in SCID Mice Treated with Vaccinia Immune Globulin ?  

OpenAIRE

A recently reported case of progressive vaccinia (PV) in an immunocompromised patient has refocused attention on this condition. Uniformly fatal prior to the licensure of vaccinia immune globulin (VIG) in 1978, PV was still fatal in about half of VIG-treated patients overall, with a greater mortality rate in infants and children. Additional therapies would be needed in the setting of a smallpox bioterror event, since mass vaccination following any variola virus release would inevitably result...

Fisher, R. W.; Reed, J. L.; Snoy, P. J.; Mikolajczyk, M. G.; Bray, M.; Scott, D. E.; Kennedy, M. C.

2010-01-01

146

Receptors for cold-insoluble globulin (plasma fibronectin) on human monocytes  

OpenAIRE

This investigation focused on the role played by cold-insoluble globulin (CIg, plasma fibronectin) in monocyte function. Surface-bound CIg mediated a concentration-dependent of human blood monocytes to gelatin-coated surfaces. CIg also mediated the binding of gelatin- coated particles such as latex beads or tanned erythrocytes to surface- bound human monocytes. However, CIg did not mediate particle ingestion. Subfractionated CIg that was highly enriched in monomeric forms (zone II CIg, mol wt...

1981-01-01

147

COMPARATIVE ELECTROPHORETIC PATTERNS OF ALBUMINS/ GLOBULINS EXTRACTED FROM DRY GRAINS AND GREEN MALTS OF BARLEY VARIETIES  

Directory of Open Access Journals (Sweden)

Full Text Available Applicability of electrophoretic separations of albumins/globulins, followed by nonspecific protein staining and specific glycoprotein and aminopeptidase detection has been examined for barley variety discrimination. Albumins/globulins extracted from dry grains and green malt of six barley varieties were separated by polyacrylamide gel electrophoresis (10 % T, pH=8.9 of native and sodium dodecyl sulfate denatured proteins, and by isoelectric focusing in pH gradient of 3.5-9.5 and 4.0-6.5. Analysis of dry grain extracts gave better results than green malt extracts. Obtained data indicate that SDS-PAGE of albumins/globulins and IEF in pH gradient 3.5-9.5 followed by Coomassie Blue staining could be useful in Croatian barley variety discrimination. Angora barley could be clearly distinguished from the other varieties which were grouped as follows: Rodnik/Sladoran, Barun/Rex, and Martin. Glycoprotein patterns did not improve the recognition of individual varieties. Broad specificity, phenylalanine and leucine preferring, and arginine specific aminopeptidase were not found as applicable markers for discrimination of examined barley varieties.

Ivica Strelec

2012-12-01

148

Thyroxine-binding globulin radioimmunoassay using a purified standard. Comparison of the results with those obtained from the maximal binding capacity of serum from thyroxine  

International Nuclear Information System (INIS)

The authors studied the binding of thyroxine in the serum. They determined from the analysis of the curvilinear Scatchard plot the affinity constant between thyroxine-binding globulin and thyroxine, and the maximal thyroxine binding capacity of thyroxine-binding globulin. After having prepared a highly purified thyroxine-binding globulin standard the authors determined the serum concentration of this protein by a radioimmunoassay. They compared the results of this assay with the results obtained from the maximal binding capacity for thyroxine. They found these two different determinations of thyroxine binding globulin concentration, which were analyzed as a function of the results reported in various papers, to be in excellent agreement

149

Thyroxine-binding globulin radioimmunoassay using a purified standard. Comparison of the results with those obtained from the maximal binding capacity of serum from thyroxine  

Energy Technology Data Exchange (ETDEWEB)

The authors studied the binding of thyroxine in the serum. They determined from the analysis of the curvilinear Scatchard plot the affinity constant between thyroxine-binding globulin and thyroxine, and the maximal thyroxine binding capacity of thyroxine-binding globulin. After having prepared a highly purified thyroxine-binding globulin standard the authors determined the serum concentration of this protein by a radioimmunoassay. They compared the results of this assay with the results obtained from the maximal binding capacity for thyroxine. They found these two different determinations of thyroxine binding globulin concentration, which were analyzed as a function of the results reported in various papers, to be in excellent agreement.

Crouzat-Reynes, G.; Lecureuil, M.; Hoinard, C.; Lejeune, B. (Faculte des Sciences Pharmaceutiques, Tours (France))

1981-01-01

150

Isolation, characterization and radioimmunoassay of corticosteroid-binding globulin (CBG) in human serum - clinical significance and comparison to thyroxine-binding globulin (TBG)  

International Nuclear Information System (INIS)

Isolation of the corticosteroid-binding globulin CBG was achieved by 5 chromatographical steps on cortisol Sepharose, QAE-Sephadex A-50, Con A-Sepharose and hydroxylapatite. The purity of the isolated CBG was demonstrated in polyacrylamide gel electrophoresis, SDS electrophoresis, immunodiffusion and ultracentrifugation. Microheterogeneity was shown in isoeletric focusing by 5 bands in the pH range of 3.7-4.2, which could be reduced to one major band after neuraminidase treatment. The equimolar binding of cortisol to CBG was demonstrated by binding studies. The association constant for cortisol was 2.8 x 108M-1, for progesterone 1.7 x 106M-1. From analytical ultracentrifugation, the molecular weight was calculated on 50 700; the sedimentation coefficient was 3.6 S, the partial specific volume 0.690 ml/g, the Stokes radius 38 A and the frictional coefficient ratio 1.5. A specific radioimmunoassay for CBG was established using the purified CBG for immunization, radioiodination and for calibration standards. The normal range of CBG levels in human serum was 2.4-4.4 mg/100 ml (mean +- 2SD). Studies were performed to compare the levels of CBG and thyroxine-binding globulin (TBG). No sex differences but a significant biphasic age dependence were observed for both proteins. In pregnancy and under oestrogen treatment of women and men, CBG was demonstrated to be the more distinct indicator of oestrogenic activity as compared with TBG oestrogenic activity as compared with TBG, whereas the sensitivity of TBG was more pronounced to supposedly antioestrogenic substances like Danazol, and in severe disease. No coincidence of genetic CBG and TBG deficiencies have been found so far. (author)

151

Comparison of rabbit antithymocyte globulin and Jurkat cell-reactive anti-T lymphocyte globulin as a first-line treatment for children with aplastic anemia.  

Science.gov (United States)

The purpose of this study was to investigate the effects of rabbit antihuman thymocyte globulin (R-ATG) and Jurkat cell-reactive anti-T lymphocyte globulin (ATG-F) in the treatment of childhood aplastic anemia (AA) and compare their efficacy and side effects. A total of 53 children with AA were analyzed in the present study, including 32 cases of severe AA, 10 cases of very severe AA and 11 cases of transfusion-dependent nonsevere AA. While receiving immunosuppressive therapy (IST), 29 and 24 patients, all of whom received long-term oral supplement with cyclosporin A (CSA), androgen, and traditional Chinese medicines, were treated with R-ATG and ATG-F, respectively. If necessary, the patients were also given supportive care such as component transfusion and/or infection control. Absolute counts of peripheral blood lymphocyte at various time points were dynamically measured after ATG therapy. According to the International AA Treatment and Effect standards, we found that there were no statistically significant differences in the response rate (70.83% vs. 68.97%, p > 0.05) and the overall survive rate (83.33% vs. 82.76%, p > 0.05) between the ATG-F and R-ATG groups. In addition, no obvious differences were observed between these two groups in the response time, efficacy in severe AA and very severe AA, or the incidence rates of ATG-related adverse reactions. After ATG treatment, the extent of peripheral blood lymphocyte reduction and duration in peripheral blood were similar between the ATG-F and R-ATG groups. The results of this study showed that ATG-F and R-ATG had similar efficacy and adverse reactions in the first-line treatment of childhood AA, despite being derived from different immunogens. PMID:24582815

Xie, Xiaotian; Shi, Wei; Zhou, Xiaoxun; Shao, Yuexia; Qiao, Xiaohong

2014-06-01

152

Protein Pattern Analysis of Blood Globulin to Estimation of Genetic Distance among Population of Tegal, Magelang, and Mojosari Duck  

Directory of Open Access Journals (Sweden)

Full Text Available The research to estimation gene frequency on the protein pattern on blood globulin and to estimation genetic distance among population Tegal, Magelang and Mojosari duck. The material were blood samples from 30 heads of duck. Electroforesis technique was used Sodium Dedocyl Sulfate (SDS polyacrilamide cel elekctroforesis according to Deutcher (1990 method with instrument of polyacrilamide electroforesis vertical system (Mini-protean II, Bio-Rad was used for analysis protein of blood globulin. The gene frequency was estimated according to direct account method and tested for Hardy-Weinberg equilibrium, whereas the genetic distance was estimated by Nei (1972. Result the analysis of electroforesis showed that locus of globulin on the local duck population was controlled by three kind of gene. The gene frequency og GlbA GlbB and GlbC was about 0.05-0.50, migration distance was about 23-60 mm. The genetic distance among Tegal, Magelang and Mojosari duck as 0.010. result of the research was concluded that the protein pattern of blood globulin is usable to estimate both genetic distance and the relationship among population of Tegal, Magelang, and Mojosari duck and also it is estimated that the geographyc location represents a factor that influence the genetic distance. (Animal Production 7(3: 177-184 (2005 Key Words : Blood Globulin, Genetic Distance, Duck Population

AT Sadewo

2005-11-01

153

Semi-quantitative immunohistochemical analysis of male rat-specific alpha2u-globulin accumulation for chemical toxicity evaluation.  

Science.gov (United States)

We purified male rat urinary alpha(2u)-globulin, prepared the antibody in rabbits, and improved an immunohistochemical detection method using this antibody for male rat-specific alpha(2u)-globulin accumulation appearing as hyaline droplets in the kidneys. Our prepared antibody reacted specifically with alpha(2u)-globulin in both immunohistochemical and Western blotting analyses, furthermore, and the graded immuno-reactivities on the slide were well associated with computational image analyzing results. Using this method, we retrospectively analyzed the renal sections from the toxicity studies of 12 nephrotoxic chemicals, which had already been conducted under the Japanese Existing Chemicals Survey Program. We demonstrated that the hyaline droplets induced by treatment with 10 chemicals (1,4-dibromobenzene, dicyclopentadiene, 3,4-dimethylaniline, 1,4-dicyanobenzene, tetrahydrothiophene-1,1-dioxide, 1,3-dicyanobenzene, acenaphthene, 3,4-dichloro-1-butene, 3a,4,7,7a-tetrahydro-1H-indene and 3,5,5-trimethylhexan-1-ol) were directly associated with alpha(2u)-globulin accumulation. This immunohistochemical method is convenient for applying, even retrospectively, paraffin sections from general toxicity studies and could be useful for qualifying male rat-specific hyaline droplets consisting of alpha(2u)-globulin and renal risk in humans. PMID:16538042

Hamamura, Masao; Hirose, Akihiko; Kamata, Eiichi; Katoku, Koshiro; Kuwasaki, Emiko; Oshikata, Takafumi; Nakahara, Yutaka; Ema, Makoto; Hasegawa, Ryuichi

2006-02-01

154

Structure of ?-conglutin: insight into the quaternary structure of 7S basic globulins from legumes.  

Science.gov (United States)

?-Conglutin from lupin seeds is an unusual 7S basic globulin protein. It is capable of reducing glycaemia in mammals, but the structural basis of this activity is not known. ?-Conglutin shares a high level of structural homology with glycoside hydrolase inhibitor proteins, although it lacks any kind of inhibitory activity against plant cell-wall degradation enzymes. In addition, ?-conglutin displays a less pronounced structural similarity to pepsin-like aspartic proteases, but it is proteolytically dysfunctional. Only one structural study of a legume 7S basic globulin, that isolated from soybean, has been reported to date. The quaternary assembly of soybean 7S basic globulin (Bg7S) is arranged as a cruciform-shaped tetramer comprised of two superposed dimers. Here, the crystal structure of ?-conglutin isolated from Lupinus angustifolius seeds (LangC) is presented. The polypeptide chain of LangC is post-translationally cleaved into ? and ? subunits but retains its covalent integrity owing to a disulfide bridge. The protomers of LangC undergo an intricate quaternary assembly, resulting in a ring-like hexamer with noncrystallographic D3 symmetry. The twofold-related dimers are similar to those in Bg7S but their assembly is different as a consequence of mutations in a ?-strand that is involved in intermolecular ?-sheet formation in ?-conglutin. Structural elucidation of ?-conglutin will help to explain its physiological role, especially in the evolutionary context, and will guide further research into the hypoglycaemic activity of this protein in humans, with potential consequences for novel antidiabetic therapies. PMID:25664733

Czubinski, Jaroslaw; Barciszewski, Jakub; Gilski, Miroslaw; Szpotkowski, Kamil; Debski, Janusz; Lampart-Szczapa, Eleonora; Jaskolski, Mariusz

2015-02-01

155

Narbonin, a 2 S globulin from Vicia narbonensis L. Crystallization and preliminary crystallographic data.  

Science.gov (United States)

A seed globulin from Vicia narbonensis L. has been crystallized by vapour diffusion induced pH-shift. Crystals are suitable for high-resolution X-ray structural analysis and diffract to better than 1.5 A. Narbonin crystallizes in the monoclinic space group P21 with alpha = 46.9 A, b = 75.5 A, c = 50.9 A, alpha = gamma = 90 degrees, beta = 120.5 degrees. The protein consists of one polypeptide chain that does not coincide with the subunits of legumin or vicilin after SDS/polyacrylamide gel electrophoresis and has a relative molecular mass of about 33,000. PMID:2231708

Hennig, M; Schlesier, B; Pfeffer, S; Höhne, W E

1990-10-01

156

In vitro Liver Synthesis and Plasma Levels of Corticosteroid-binding Globulin (CBG) in the Piglet  

OpenAIRE

In vitro production of corticosteroid-binding globulin (CBG) was evaluated in liver collected from piglets at 3 to 40 days of age and compared to the developmental pattern of plasma levels of CBG. The amount of CBG released per unit weight of liver slices and in circulation was measured using an enzyme-linked immunosorbent assay (ELISA) procedure. Plasma CBG levels were low on days 3 and 10, but significantly (P < 0.05) increased on day 30. The concentration of CBG released from live...

Jeonghoon Heo; Kattesh, Henry G.; Dorz, Jules J. E.; Roberts, Mary P.; Godkin, James D.

2003-01-01

157

Systemic lupus erythematosis with severe aplastic anemia successfully treated with rituximab and antithymocyte globulin  

Science.gov (United States)

Hematologic disorders are very common in Systmic lupus erythematosus (SLE).First presentation of SLE with severe aplastic anemia (SAA) is extremely rare. We report a patient with the diagnosis of secondary SAA associated with SLE. Conventional therapy was not effective. She received Rituximab (RTX) and Antithymocyte globulin (ATG) therapy, her response was satisfactory finally. Her hematologic parameters were within normal ranges until last follow-up, eight months and six months after therapy was initiated with RTX and ATG, respectively. This is the first time RTX and ATG were successfully used in the treatment of SAA secondary to SLE. PMID:24772161

Liu, Wenbin; Hu, Zhiping; Lin, Shengyun; He, Jianghua; Zhou, Yuhong

2014-01-01

158

Specific binding of human corticosteroid-binding globulin to cell membranes.  

OpenAIRE

Specific binding sites for corticosteroid-binding globulin were detected on membranes prepared from human prostates. The binding sites are typical of membrane receptors: they are saturable and specific and have high affinity. There was little specific binding at 4 degrees C and 23 degrees C. Maximal specific binding was obtained at 37 degrees C. Scatchard analysis revealed the presence of a single set of binding sites with an apparent dissociation constant of 8.7 X 10(-7) M and a binding capa...

Hryb, D. J.; Khan, M. S.; Romas, N. A.; Rosner, W.

1986-01-01

159

Cloning and sequence of several alpha 2u-globulin cDNAs.  

OpenAIRE

We describe a simple cloning procedure for alpha 2u-globulin that requires neither enrichment of mRNA for cloning nor purification of a specific probe for screening recombinant colonies. Total adult male liver poly(A)+RNA was used as template for cloning, and the subsequent recombinant colonies were screened by comparing hybridization to radioactive cDNA probes prepared from hepatic male and female mRNA, respectively. Almost all of the selected "male-specific" clones were later shown to conta...

Unterman, R. D.; Lynch, K. R.; Nakhasi, H. L.; Dolan, K. P.; Hamilton, J. W.; Cohn, D. V.; Feigelson, P.

1981-01-01

160

Proposal of abolition of the skin sensitivity test before equine rabies immune globulin application  

OpenAIRE

An epizootic outbreak of rabies occurred in 1995 in Ribeirão Preto, SP, with 58 cases of animal rabies (54 dogs, 3 cats and 1 bat) confirmed by the Pasteur Institute of São Paulo, and one human death. The need to provide care to a large number of people for the application of equine rabies immune globulin (ERIG) prevented the execution of the skin sensitivity test (SST) and often also the execution of desensitization, procedures routinely used up to that time at the Emergency Unit of the Un...

Cupo, Palmira; Azevedo-marques, Marisa M.; Sarti, Willy; Hering, Sylvia Evelyn

2001-01-01

161

Comparative characterization of molecular varieties of thyroxine-binding human globulin  

Energy Technology Data Exchange (ETDEWEB)

Two molecular varieties of thyroxine-binding globulin (TBG) of human retroplacental blood, obtained as a result of fractionation of pure TBG on concanavalin A-Sepharose, were studied. It was shown that these varieties (TBG-1 and TBG-2) are immunologically identical; they have the same molecular weight and amino acid composition, exhibit the same affinity for thyroid hormones, and are indistinguishable in spectral characteristics. And yet, TBG-1 and TBG-2 have differences in charge, detectable in isoelectrofocusing, and a different monosaccharide composition. The existence of molecular varieties of TBG during pregnancy is apparently due to the peculiarities of the glycosylation of the polypeptide chain during TBG biosynthesis.

Ermolenko, M.N.; Sviridov, O.V.; Strel' chenok, O.A.

1986-06-10

162

Labeling of hematoporphyrin with sup 105 Rh and binding studies with human gamma globulin  

Energy Technology Data Exchange (ETDEWEB)

Labelling of hematoporphyrin with {sup 105}Rh at stoichiometric concentrations is described. Labelling efficiencies of up to 93% could be obtained at pH 9.0 in bicarbonate buffer. Solvent extraction of {sup 105}Rh-hematoporphyrin into methyl isobutyl ketone was used to estimate the complex yield. The complex showed high stability and no loss of {sup 105}Rh was seen throughout the 6 days of study. {sup 105}Rh-hematoporphyrin when incubated with human {gamma} globulin was seen to be quantitatively bound to the protein. This procedure may be used for labelling monoclonal antibodies with {sup 105}Rh for therapeutic applications. (author).

Pillai, M.R.A.; Lo, J.M.; Troutner, D.E. (Missouri Univ., Columbia, MO (USA). Dept. of Chemistry)

1990-01-01

163

Mathematical model for determination of colloid osmotic pressure: The role of albumin-globulin ratio  

OpenAIRE

BACKGROUND: Colloid Osmotic Pressure (COP) is an important factor in the fluid balance of body compartments. COP is related to Total Protein (TP) concentration and Albumin: Globulin Ratio (A/G). The A/G was not included in pervious empirical models, and therefore the main objective of this study was to develop a mathematical model to determine the COP in terms of TP concentration and A/G. METHODS: Sera with different A/G were prepared in-vitro, and COP was measured directly using colloid...

Mehdi Nematbakhsh; Ali Moradi; Majid Khazaei; Somaieh Jafari

2006-01-01

164

Rhesus and African green monkey leukocyte response to steroids, antithymocyte globulin, and /sup 60/Co treatments  

Energy Technology Data Exchange (ETDEWEB)

Rhesus and African green monkeys were treated with dexamethasome, antithymocyte globulin, /sup 60/Co irradiation, or combinations of those immunosuppressive agents, and the leukocyte response was followed. The lymphocyte population could be significantly depressed with /sup 60/Co, but dexamethasone was required to maintain a low lymphocyte count. The ability of the immunosuppressed animals to support the growth of transplanted tumor cells was markedly enhanced by the addition of ATG to the treatment schedule, even though ATG had no effect on the numbers of leukocytes in the peripheral circulation.

Petricciani, J.C.; Gillette, R.

1976-02-01

165

Rhesus and African green monkey leukocyte response to steroids, antithymocyte globulin, and 60Co treatments  

International Nuclear Information System (INIS)

Rhesus and African green monkeys were treated with dexamethasome, antithymocyte globulin, 60Co irradiation, or combinations of those immunosuppressive agents, and the leukocyte response was followed. The lymphocyte population could be significantly depressed with 60Co, but dexamethasone was required to maintain a low lymphocyte count. The ability of the immunosuppressed animals to support the growth of transplanted tumor cells was markedly enhanced by the addition of ATG to the treatment schedule, even though ATG had no effect on the numbers of leukocytes in the peripheral circulation

166

Tritium storage  

International Nuclear Information System (INIS)

This document represents a synthesis relative to tritium storage. After indicating the main storage particularities as regards tritium, storages under gaseous and solid form are after examined before establishing choices as a function of the main criteria. Finally, tritium storage is discussed regarding tritium devices associated to Fusion Reactors and regarding smaller devices

167

Variation of Seed Storage Protein Electrophoretic Patterns in Mungbean  

Directory of Open Access Journals (Sweden)

Full Text Available Variability of seed storage protein electrophoretic patterns revealed by SDS-Polyacrylamide gel electrophoresis and their possible relations with some morphological and phenological characteristics were studied using 193 accessions from Iran Mungbean (Vigna radiata L. Wilczek Collection. Seed protein electrophoresis revealed six different patterns that were different in two albumin and four globulin subunits with molecular weights of 23500-34000. Pattern types 1 and 2 had a considerable frequency but pattern types 3, 4, 5 and 6 had low frequencies and probably had developed recently in mungbean evolution process. Cluster analysis of countries and cities using the frequencies of albumin and globulin subunits showed no relation between geographical diversity and similarity distances. The correlations of morphological traits and albumin and globulin subunits indicated a relationship between G1 and G2 subunits with 1000 seed weight and days to beginning of maturity. Therefore, selection based on protein patterns at preliminary stages of breeding programs might be effective in increasing seed yield together with earliness.

Ahmad Arzani

1999-07-01

168

Intraindividual variation of triiodothyronine, thyroxine, thyrotropin and thyroxine-binding globulin in fasting serum from healthy men  

Energy Technology Data Exchange (ETDEWEB)

The concentrations of triiodothyronine, thyroxine, thyrotropin and thyroxine-binding globulin were determined in fasting serum from 11 healthy men (age 18-25 years) by radioimmunoassays conducted over a period of 4 weeks on 5 consecutive days per week. The concentrations of thyroxine and thyroxine-binding globulin were very consistent intraindividually, with coefficients of variation of 7.84% and 9.37%, respectively. The triiodothyronine and thyrotropin levels showed significant intraindividual variability with coefficients of variation of 18.38% and 51.85%, respectively. These results point to the type of difficulties encountered in judging serum values, namely intraindividual variations over a given period of time.

Liappis, N.; Hoffmann, U.; Rao, M.L.

1986-02-01

169

In vitro thyroid testing in populations with low thyroxine binding globulin capacity  

International Nuclear Information System (INIS)

Total thyroxine (T4) concentration in serum is a reliable indicator of thyroid function in most individuals, but it is affected by altered concentrations of thyroxine-binding globulin (TBG) in serum. Within certain limits, the variations in total TBG binding capacity (TBGTOTAL) caused by the fluctuations in the concentration of this binding globulin in serum can be modulated by calculating the free thyroxine index (FT4I) as the product of T4 and the in vitro uptake of triiodothyronine by a secondary binder (T3U). This calculation is empirically based on the facts that free TBG binding capacity (TBGFREE) is inversely related to T3U and that T4 and T3U show opposite behaviour when measured in sera with altered TBG: a low T4 in serum with reduced TBGTOTAL is compensated by a high value for T3U, while an elevated T4 in serum with increased TBGTOTAL is compensated by a low value for T3U. In both cases the product of T4 and T3 renders a normal FT4I value, showing a certain association with the concentration of free T4 in serum (FT4). In fact, this index has been shown to be superior than several FT4 assay systems in the assessment of thyroid status in clinical euthyroid subjects with relatively high or low T3U

170

Expression of Corticosteroid-Binding Globulin CBG in the Human Heart.  

Science.gov (United States)

Glucocorticoids are known to be involved in myocardial regeneration and destruction. Cardiomyocytes are mostly devoid of nuclear glucocorticoid receptors (GRs) and it is generally assumed that effects of adrenal steroids in heart are mediated through the mineralocorticoid receptor (MR). Here we used immunocytochemistry to study localization of corticosteroid binding globulin (CBG) in semithin sections of human cardiac tissue samples. With staining of consecutive sections we examined colocalization with GR and MR immunoreactivities. While GR staining was almost undetectable, a portion of myocytes with MR immunostained nuclei was found. Almost all cardiomyocytes exhibited CBG immunostaining in cytoplasm and on the cell membrane. Most pronounced CBG immunoreactivities were found in Purkinje fibers and in smooth muscle cells of arterial walls. With RT-PCR, we found in homogenates of cardiac tissue detectable levels of CBG encoding mRNA. Our findings indicate that CBG is expressed in human heart. Known cardiac effects of adrenal steroids may in part be mediated through the binding globulin and its putative membrane receptor in addition to nuclear steroid receptors and direct genomic action. Highlights of our study: Human cardiomyocytes express mineralocorticoid receptors, but are mostly free of nuclear glucocorticoid receptors. CBG is expressed in myocardium and in Purkinje fibers. CBG in heart is colocalized with mineralocorticoid receptor. Endothelia and smooth muscle cells of arterial walls show colocalization of CBG and MR. PMID:25251318

Schäfer, H H; Gebhart, V M; Hertel, K; Jirikowski, G F

2014-09-24

171

Effect of total binding capacity of thyroxine binding globulin on the free thyroxine index  

Energy Technology Data Exchange (ETDEWEB)

In search of a definite source of misleading free thyroxine index (FT/sub 4/I), the relationship between in vitro thyroid testing results and thyroxine-binding globulin (TBG) capacities were reexamined in sera from a population with a relatively high prevalence of serum TBG alterations. Sera from 21 subjects with different total thyroxine-binding globulin capacities (TTBG), were loaded with graded amounts of thyroxine (T/sub 4/) and assayed for T/sub 4/, T/sub 3/ uptake (T/sub 3/U), TTBG, and free T/sub 4/ concentration (FT/sub 4/I). Serum T/sub 4/, T/sub 3/U, and the calculated FT/sub 4/ index (FT/sub 4/I) were able to separate efficiently the samples according to their FT/sub 4/, but their respective normal ranges varied with TTBG. Interpretation of the results of the in vitro tests, in the light of TTBG, greatly improved their operating characteristics in the study of 141 patients with a high prevalence of TBG alterations. The misleading FT/sub 4/I is not the outcome of reduced intrinsic sensitivities of the in vitro tests, but a consequence of a shift of their normal ranges caused by a change of TTBG. By estimating TTBG from the values of T/sub 4/ and T/sub 3/U, this problem is easily solved without adding cost.

Cuaron, A.

1986-06-01

172

Radioimmunoassay of testosterone and of sexual hormone-binding globulin in plasma of women with hirsutism  

International Nuclear Information System (INIS)

Plasma-borne testosterone was determined in 176 women with hirsutism, and in 47 patients sexual hormone-binding globulin was determined as well. The highest average testosterone values were recorded from cases with congenital adrenogenital syndrome (AGS). In cases of postnatal AGS values were much lower, but they were clearly in excess of those recordable from Stein-Leventhal syndrome. Plasma borne testosterone in cases of hirsutism came very close to testosterone levels established in the context of Stein-Leventhal syndrome. Testosterone levels dropped with significance, following AGS treatment, using cortisol derivatives, and following wedge-shaped ovariectomy. Sexual hormone binding-globulin was found to be strongly reduced in almost all women with hirsutism. Such reduction seemed to suggest the presence of increased amounts of free active testosterone in the blood of those patients. Determination of plasma-borne testosterone in cases of hirsutism is considered to be essential to both diagnosis of the endocrinological syndromes and monitoring of therapy. (author)

173

Rabbit antithymocyte globulin (Thymoglobulin®) impairs the thymic output of both conventional and regulatory CD4+ T cells after allogeneic hematopoietic stem cell transplantation in adult patients  

Science.gov (United States)

Rabbit antithymocyte globulin-Genzyme™ is used to prevent graft-versus-host disease after allogeneic hematopoietic stem cell transplantation. Common disadvantages of treatment are infectious complications. The effects of rabbit antithymocyte globulin-Genzyme™ on thymic function have not been well-studied. Multicolor flow cytometry was used to analyze the kinetics of conventional and regulatory T cells in adult patients treated (n=12) or not treated (n=8) with rabbit antithymocyte globulin-Genzyme™ during the first 6 months after allogeneic hematopoietic stem cell transplantation. Patients treated with rabbit antithymocyte globulin-Genzyme™ had almost undetectable levels of recent thymic emigrants (CD45RA+CD31+) of both conventional and regulatory CD4T cells throughout the 6 months after allogeneic hematopoietic stem cell transplantation whereas CD4+CD45RA-memory T cells were less affected, but their levels were also significantly lower than in patients not treated with rabbit antithymocyte globulin-Genzyme™. In vitro, rabbit antithymocyte globulin-Genzyme™ induced apoptosis and cytolysis of human thymocytes, and its cytotoxic effects were greater than those of rabbit antithymocyte globulin-Fresenius™. Rabbit antithymocyte globulin-Genzyme™ in combination with a conditioning regimen strongly impairs thymic recovery of both conventional and regulatory CD4+ T cells. The sustained depletion of conventional and regulatory CD4+T cells carries a high risk of both infections and graft-versus-host disease. Our data indicate that patients treated with rabbit antithymocyte globulin-Genzyme™ could benefit from thymus-protective therapies and that trials comparing this product with other rabbit antithymocyte globulin preparations or lymphocyte-depleting compounds would be informative. PMID:22801968

Na, Il-Kang; Wittenbecher, Friedrich; Dziubianau, Mikalai; Herholz, Anne; Mensen, Angela; Kunkel, Désirée; Blau, Olga; Blau, Igor; Thiel, Eckhard; Uharek, Lutz; Scheibenbogen, Carmen; Rieger, Kathrin; Thiel, Andreas

2013-01-01

174

Underground storage  

International Nuclear Information System (INIS)

This meeting is divided into three parts: In the first part, 9 papers describe the problems connected with petroleum, natural gas or liquefied gas storage in geologic deposits. In the second part, the problems connected with radioactive waste disposal in deep geologic deposits are described. 10 papers are in the INIS scope. In the third part, another forms of storage are studied (hazardous materials storage, heat storage)

175

[Human i.v. immune globulin by chemical modification with beta-propiolactone/radiochemical study (author's transl)].  

Science.gov (United States)

Elimination of anticomplementary activity of human immune globulin G (IgG, Intraglobin) is achieved by introduction of 6 OH-propionic groups per IgG-molecule. These groups introduced by modification with beta-propiolactone, are equally spread out on the molecule and situated at lysin. PMID:7194066

Stephan, W; Fasold, H

1980-01-01

176

High percentage of ?1-globulin in serum protein is associated with unfavorable prognosis in non-small cell lung cancer.  

Science.gov (United States)

The association of the percentage composition of serum protein in patients undergoing lung resections for non-small cell lung cancer (NSCLC) with overall survival and recurrence-free survival has never been investigated. Patients were selected consecutively from the database of the Bio-Bank of Shandong Provincial Hospital. We retrospectively examined the impact of preoperative percentage composition of serum protein detected by serum protein electrophoresis on survival. Furthermore, we investigated the relationships between the potential prognostic biomarkers and clinicopathological factors. A total of 390 patients were evaluated. The higher percentage of ?1-globulin in serum protein was significantly associated with histology type (p<0.001), worse tumor status (p<0.001) and higher pathological stage (p=0.004). The ?1-globulin percentage composition was an independent prognostic factor for overall survival (hazard ratio 1.52, 95% CI 1.04-2.23, p=0.03). High percentage of ?1-globulin in serum protein was also related to short recurrence survival (hazard ratio 1.56, 95% CI 1.14-2.13, p=0.005). Our results showed that the percentage of ?1-globulin in serum protein may be an independent prognostic factor in NSCLC. PMID:25216870

Qu, Xiao; Pang, Zhaofei; Yi, Weiwei; Wang, Ying; Wang, Kai; Liu, Qi; Du, Jiajun

2014-10-01

177

Influence of administered indigenous microorganisms on uptake of (Iodine-125). gamma. -globulin in vivo by intestinal segments of neonatal calves  

Energy Technology Data Exchange (ETDEWEB)

Ten calves less than 14 h of age (average 8.6 h) were anaesthetized, and the intestine was ligated into segments 10 cm in length at 3-cm intervals beginning 1.8 m anterior to the ileocecal junction and proceeding proximally. Seven treatments were assigned in random order to segments in three successive sections of the small intestine. Segments received 1 ml of viable bacteria of intestinal origin, autoclaved bacteria of intestinal origin, or sterile microbiological broth at zero time; then after 4 h they were injected with iodine-125 labeled ..gamma..-globulin. After an additional 1.5 h, the experiment was ended and uptake assayed. Two treatments measured anaerobic microbial growth after 4 h incubation with 1 ml of either sterile broth or live bacteria culture. Residual (iodine-125)..gamma..-globulin was measured in segments receiving 1 ml of sterile broth or live bacteria culture with 5.5-h incubation followed by 15-s exposure to labeled ..gamma..-globulin. Uptake was lowest in segments receiving live bacteria as compared to segments receiving sterile inocula. Number of bacteria per gram of tissue was correlated negatively with uptake. Low corticosteroids in serum were associated with low uptake of ..gamma..-globulin.

James, R.E.; Polan, C.E.; Cummings, K.A.

1981-01-01

178

Influence of administered indigenous microorganisms on uptake of (iodine-125). gamma. -globulin in vivo by intestinal segments of neonatal calves  

Energy Technology Data Exchange (ETDEWEB)

Ten calves less than 14 h of age (average 8.6 h) were anaesthetized, and the intestine was ligated into segments 10 cm in length at 3-cm intervals beginning 1.8 m anterior to the ileocecal junction and proceeding proximally. Seven treatments were assigned in random order to segments in three successive sections of the small intestine. Segments received 1 ml of viable bacteria of intestinal origin, autoclaved bacteria of intestinal origin, or sterile microbiological broth at zero time; then after 4 h they were injected with iodine-125 labeled ..gamma..-globulin. After an additional 1.5 h, the experiment was ended and uptake assayed. Two treatments measured anaerobic microbial growth after 4 h incubation with 1 ml of either sterile broth or live bacteria culture. Residual (iodine-125) ..gamma..-globulin was measured in segments receiving 1 ml of sterile broth or live bacteria culture with 5.5-h incubation followed by 15-s exposure to labeled ..gamma..-globulin. Uptake was lowest in segments receiving live bacteria as compared to segments receiving sterile inocula. Number of bacteria per gram of tissue was correlated negatively with uptake. Low corticosteroids in serum were associated with low uptake of ..gamma..-globulin.

James, R.E.; Polan, C.E.; Cummins, K.A.

1981-01-01

179

Effects of low doses of x-radiation on nonspecific defense systems and gamma globulin concentration in chick blood  

Energy Technology Data Exchange (ETDEWEB)

The effects of low doses of x radiation on the concentration of gamma globulins in blood and protein complexes absorbed on zymosan in chickens were investigated. Studies demonstrated that low doses of ionizing radiation enhanced the bactericidal properties and virus neutralizing properties of blood serum.

Rudakov, V.V.; Tsikina, N.V.

1978-01-01

180

Sex Hormone-Binding Globulin Genetic Variation: Associations with Type 2 Diabetes Mellitus and Polycystic Ovary Syndrome  

OpenAIRE

Sex hormone-binding globulin (SHBG) is the primary plasma transport protein for sex steroid hormones and regulates the bioavailability of these hormones to target tissues. The gene encoding SHBG is complex and any of several polymorphisms in SHBG have been associated with alterations in circulating SHBG levels.

Chen, Chen; Smothers, Jamie C.; Lange, Allison; Nestler, John E.; Strauss, Jerome F.; Wickham, Edmond P.

2010-01-01

181

Self-Assembly of Nano Hydroxyapatite or Aragonite Induced by Molecular Recognition to Soy Globulin 7S or 11S.  

Science.gov (United States)

Molecular self-assembly is emerging as a viable 'bottom-up' approach to build stable organic/inorganic nanometer-scale blocks. Herein, under the conditions of appropriate pH and ionic strength, soy globulin 7S or 11S were coprecipitated with hydroxyapatite (HAp) or aragonite (Arag), respectively, to fabricate two organic/inorganic hybrids: 7S/HAp and 11S/Arag. Results from high-resolution transmission electron microscopy show that the hybrids exhibit a nanosized core-shell structure with globulin monomer 7S or 11S as core and HAp or Arag as shells. 7S/HAp and 11S/Arag present a disk and hexagon shape, respectively. After calcinations, monodispersed HAp without support from globulins existed as nanospheres. It was revealed that the globulin as host induces the self-assembly and growth layer by layer of HAp or Arag nanocrystals. The factors of molecular recognition and surface potential definitely affected the size and shape of the hierarchical blocks. This work provided a novel pathway to controllably synthesize a wide variety of precise plant protein/biomineral hybrid biomaterials. PMID:21638411

Liu, Dagang; Tian, Huafeng; Kumar, Rakesh; Zhang, Lina

2009-09-01

182

Brief Report: Dysregulated Immune System in Children with Autism: Beneficial Effects of Intravenous Immune Globulin on Autistic Characteristics.  

Science.gov (United States)

Children (ages 3-12) with autism (n=25) were given intravenous immune globulin (IVIG) treatments at 4-week intervals for at least 6 months. Marked abnormality of immune parameters was observed in subjects, compared to age-matched controls. IVIG treatment resulted in improved eye contact, speech, behavior, echolalia, and other autistic features.…

Gupta, Sudhir; And Others

1996-01-01

183

The families of papain- and legumain-like cysteine proteinases from embryonic axes and cotyledons of Vicia seeds: developmental patterns, intracellular localization and functions in globulin proteolysis.  

Science.gov (United States)

Families of papain- and legumain-like cysteine proteinases (CPR) were found in Vicia seeds. cDNAs and antibodies were used to follow organ specificity and the developmental course of CPR-specific mRNAs and polypeptides. Four papain-like cysteine proteinases (CPR1, CPR2, proteinase A and CPR4) from vetch seeds (Vicia sativa L.) were analysed. CPR2 and its mRNA were already found in dry embryonic axes. CPR1 was only detected there during early germination. Both CPR1 and CPR2 strongly increased later during germination. In cotyledons, both CPR1 and CPR2 were only observed one to two days later than in the axis. Proteinase A was not found in axes. In cotyledons it could only be detected several days after seeds had germinated. CPR4 mRNA and polypeptide were already present in embryonic axes and cotyledons during seed maturation and decreased in both organs during germination. Purified CPR1, CPR2 and proteinase A exhibited partially different patterns of globulin degradation products in vitro. Although the cDNA-deduced amino acid sequence of the precursor of proteinase A has an N-terminal signal peptide, the enzyme was not found in vacuoles whereas the other papain-like CPRs showed vacuolar localization. Four different legumain-like cysteine proteinases (VsPB2, proteinase B, VnPB1 and VnPB2) of Vicia species were analysed. Proteinase B and VnPB1 mRNAs were detected in cotyledons and seedling organs after seeds had germinated. Proteinase B degraded globulins isolated from mature vetch seeds in vitro. VsPB2 and proteinase B are localized to protein bodies of maturing seeds and seedlings, respectively, of V. sativa. Like VsPB2 from V sativa, also VnPB2 of V. narbonensis corresponds to vacuolar processing enzymes (betaVPE). Based on these results different functions in molecular maturation and mobilization of storage proteins could be attributed to the various members of the CPR families. PMID:10949376

Fischer, J; Becker, C; Hillmer, S; Horstmann, C; Neubohn, B; Schlereth, A; Senyuk, V; Shutov, A; Müntz, K

2000-05-01

184

Control of adhesion of human induced pluripotent stem cells to plasma-patterned polydimethylsiloxane coated with vitronectin and ?-globulin.  

Science.gov (United States)

Human induced pluripotent stem cells (hiPSCs) are a promising source of cells for medical applications. Recently, the development of polydimethylsiloxane (PDMS) microdevices to control the microenvironment of hiPSCs has been extensively studied. PDMS surfaces are often treated with low-pressure air plasma to facilitate protein adsorption and cell adhesion. However, undefined molecules present in the serum and extracellular matrix used to culture cells complicate the study of cell adhesion. Here, we studied the effects of vitronectin and ?-globulin on hiPSC adhesion to plasma-treated and untreated PDMS surfaces under defined culture conditions. We chose these proteins because they have opposite properties: vitronectin mediates hiPSC attachment to hydrophilic siliceous surfaces, whereas ?-globulin is adsorbed by hydrophobic surfaces and does not mediate cell adhesion. Immunostaining showed that, when applied separately, vitronectin and ?-globulin were adsorbed by both plasma-treated and untreated PDMS surfaces. In contrast, when PDMS surfaces were exposed to a mixture of the two proteins, vitronectin was preferentially adsorbed onto plasma-treated surfaces, whereas ?-globulin was adsorbed onto untreated surfaces. Human iPSCs adhered to the vitronectin-rich plasma-treated surfaces but not to the ?-globulin-rich untreated surfaces. On the basis of these results, we used perforated masks to prepare plasma-patterned PDMS substrates, which were then used to pattern hiPSCs. The patterned hiPSCs expressed undifferentiated-cell markers and did not escape from the patterned area for at least 7 days. The patterned PDMS could be stored for up to 6 days before hiPSCs were plated. We believe that our results will be useful for the development of hiPSC microdevices. PMID:24656306

Yamada, Ryotaro; Hattori, Koji; Tachikawa, Saoko; Tagaya, Motohiro; Sasaki, Toru; Sugiura, Shinji; Kanamori, Toshiyuki; Ohnuma, Kiyoshi

2014-09-01

185

Rapid postexposure decay of. cap alpha. /sub 2u/-globulin and hyaline droplets in the kidneys of gasoline-treated male rats  

Energy Technology Data Exchange (ETDEWEB)

Renal ..cap alpha../sub 2u/-globulin content increased to 210% of control within 18 h of a single oral dose of gasoline (2.0 ml/kg) in male rats; maximal levels (320% of control) were attained following gasoline administration for 3 d. Increases in renal ..cap alpha../sub 2u/-globulin caused by gasoline were accompanied by concurrent proliferation of hyaline droplets. However, within 3 d of terminating gasoline administration renal ..cap alpha../sub2u/-globulin content decreased to the same level as that in unexposed rats, although renal hyaline droplet number returned to pretreatment levels somewhat more slowly. The conjoint effect of postexposure recovery and estradiol (an inhibitor of hepatic ..cap alpha../sub 2u/-globulin synthesis) administration was also determined in male rats. On postexposure of 3, 6, and 9, estradiol treatment (1 mg/kg, sc, 4 d, starting on d 9 of gasoline treatment) decreased renal ..cap alpha../sub 2u/-globulin content to 75%, 59%, and 48%, respectively, of that in rats allowed to recover from gasoline with no hormone treatment. Hepatic ..cap alpha../sub 2u/-globulin content in estradiol-treated rats was decreased by 74%, 97%, and 96% at the same intervals. Estradiol treatment during recovery from gasoline also appeared to increase the removal of accumulated hyaline droplets from the renal cortex. Thus, accumulation of ..cap alpha../sub 2u/-globulin-containing hyaline droplets after subacute exposure of male rats to gasoline is rapidly reversible, dependent on continuous exposure to gasoline and maintenance of the normal rate of hepatic ..cap alpha../sub 2u/-globulin synthesis. These results emphasize the dynamic state of renal cortical hyaline droplets and suggest strongly that gasoline hydrocarbons cause hyaline droplet accumulation by prolonging the half-time degradation of ..cap alpha../sub 2u/-globulin.

Garg, B.D.; Olson, M.J.; Demyan, W.F.; Roy, A.K.

1988-01-01

186

The fate of vicilins, 7S storage globulins, in larvae and adult Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae).  

Science.gov (United States)

The fate of vicilins ingested by Callosobruchus maculatus and the physiological importance of these proteins in larvae and adults were investigated. Vicilins were quantified by ELISA in the haemolymph and fat body during larval development (2nd to 4th instars), in pupae and adults, as well as in ovaries and eggs. Western blot analysis demonstrated that the majority of absorbed vicilins were degraded in the fat body. Tracing the fate of vicilins using FITC revealed that the FITC-vicilin complex was present inside cells of the fat body of the larvae and in the fat bodies of both male and female adult C. maculatus. Labelled vicilin was also detected in ovocytes and eggs. Based on the results presented here, we propose that following absorption, vicilins accumulate in the fat body, where they are partially degraded. These peptides are retained throughout the development of the insects and eventually are sequestered by the eggs. It is possible that accumulation in the eggs is a defensive strategy against pathogen attack as these peptides are known to have antimicrobial activity. Quantifications performed on internal organs from larvae of C. maculatus exposed to extremely dry seeds demonstrated that the vicilin concentration in the haemolymph and fat body was significantly higher when compared to larvae fed on control seeds. These results suggest that absorbed vicilins may also be involved in the survival of larvae in dry environments. PMID:20230826

Souza, Sheila M; Uchôa, Adriana F; Silva, José R; Samuels, Richard I; Oliveira, Antônia E A; Oliveira, Eliana M; Linhares, Ricardo T; Alexandre, Daniel; Silva, Carlos P

2010-09-01

187

[Rabbit testosterone-binding globulin and its use in concurrent protein-binding analysis].  

Science.gov (United States)

The existence of testosterone-binding globulin (TBC) homogeneous by the association constant (of the 10(8) M-1 order) and sedimentation characteristics (sedimentation coefficient--4S) was demonstrated in various groups of rabbits (mature males and females, pregnant females and fetuses, immature males). The level of the TBG binding capacity failed to show any marked differences in all the ontogenetic groups. The TBG level was rather close in the rabbit mother and fetus. Estradiol, estron, progesterone, cortizol possessed no affinity to this protein. Dehydrotestosterone affinity was about three times greater than that of testosterone. When TBG was used in the competitive protein-binding analysis of testosterone and other protein-bound androgens in the blood plasma of men, women and rabbits (male and female) there were obtained results coinciding with those of other authors. This permitted to recommend TBG for wide practical use in the competitive protein-binding analysis. PMID:564060

Polyntsev, Iu V; Volchek, A G; Rozen, V B; Novikova, T G; Rastimeshin, S P

1977-01-01

188

Control survey of normal reference ranges adopted for serum thyroxine binding globulin, thyroxine, triiodothyronine in Japan  

International Nuclear Information System (INIS)

A survey using questionnaires was made on 152 facilities from July through September 1984 to examine normal reference ranges of serum thyroxine binding globulin (TBG), thyroxine (TT4), and triiodothyronine (TT3). Normal reference ranges of TBG were in good agreement with each other, with the exception of four facilities showing high upper limits. An average value of the upper and lower limits in 83 facilities was 13.7 +- 1.9 ?g/ml; and the standard deviation was 28.6 +- 2.8 ?g/ml. Differences (approximately 10 %) in coefficient of variation were comparable to those (5.7-9.6 %) obtained from the previous survey. There were approximately 10 % differences in coefficient of variation for both TT4 and TT3. (Namekawa, K.)

189

Accessibility and in situ deposition of gamma globulin onto solid rat tumors  

Energy Technology Data Exchange (ETDEWEB)

The accessibility of circulating antibody to the extravascular space of solid tumors was studied. It is known that the capillary bed of normal tissue offers resistance the free movement of plasma proteins. Since solid tumors are vascularized by a host derived endothelium and since the majority of tumor cells lie outside the vasculature, circulating antibody must first pass from the plasma across the capillaries to the extravascular fluid of the tumor in order for it to combine with antigens on tumor cells. The rate of influx of normal rat IgG from the blood into the fluid surrounding the tumor cells and the rage of efflux of IgG from the tumor back to the blood were measured. Attempts to demonstrate that gamma globulin is present on the surface of the tumor cells as a result of antibodies sticking to tumor antigens were inconclusive.

O' Connor, S.

1977-01-01

190

Detection of opsonic antibodies against Enterococcus faecalis cell wall carbohydrates in immune globulin preparations.  

Science.gov (United States)

Three different commercially available polyvalent immune globulins (IG) were investigated for the existence of antibodies against cell wall carbohydrates of four different E. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). All three IG preparations contained antibodies against all four serotypes (CPS-A to CPS-D). However, only one of the three IG preparations showed opsonic killing against all four serotypes. Average killing was higher against serotypes A and B (72 and 79 %, respectively) than against serotypes C and D (30 and 37 %, respectively). Such IG preparations could play a role as an adjuvant therapeutic option in life-threatening infections with E. faecalis, particularly when resistant strains are involved. PMID:24854332

Hufnagel, M; Sixel, K; Hammer, F; Kropec, A; Sava, I G; Theilacker, C; Berner, R; Huebner, J

2014-08-01

191

Simultaneous determination of free thyroxine and capacity of thyroxine-binding globulin  

International Nuclear Information System (INIS)

A simple method is described for the simultaneous determination of capacity thyroxine-binding of globulin (TBG) and free thyroxine concentration (FT4). The ratio of bound to free T4 (B/F) is first determined for two total-T4 concentrations using a Sephadex G-25 competitive-binding technique. TBG capacity and FT4 can both be calculated assuming a known value of affinity constant of TBG. The method is linear over a twenty-fold serum dilution. FT4 calculated is identical to that calculated using the method of Irvine. TBG capacity is shown to be linearly correlated to TBG concentration as determined by radioimmunoassay and is consistent with a molecular weight of 69,000 Daltons and one T4 binding site per molecule. FT4 is found to correlate with the free thyroxine index in a complicated way, depending on the degree of TBG saturation

192

Sex hormone-binding globulin (SHBG) and estradiol cross-talk in breast cancer cells.  

Science.gov (United States)

Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that regulates the action of steroid hormones at several levels. SHBG regulates the availability of free androgens and estradiol to hormone-responsive tissues. Moreover, SHBG is also part of a novel steroid signaling system. We report here on the mechanism of action and the biological effects of SHBG in breast cancer cells, especially distinguishing cross-talk between membrane-initiated SHBG and estradiol pathways. After interacting with a specific binding site on breast cancer cell membranes, SHBG activates a specific pathway, and by cAMP induction, inhibits estradiol-mediated activation of ERK. Both estradiol and SHBG membrane-initiated pathways involve cross-talk at MAP kinase level with the ultimate result of inhibiting estradiol-mediated cell growth and antiapoptosis. On the basis of reported evidence, we suggest that SHBG is one of the regulators of growth and apoptosis of estrogen-dependent breast cancer cells. PMID:16700004

Fortunati, N; Catalano, M G

2006-04-01

193

Expression of corticosteroid-binding globulin in human astrocytoma cell line.  

Science.gov (United States)

Glial tumor cells are known to be sensitive to glucocorticoids (GC) in vivo and in vitro. Here we studied the expression of corticosteroid-binding globulin (CBG) in the low-grade malignant human astrocytoma cell line 1321N1. CBG was observed in cytoplasm of most of these cells with immunocytochemistry. RT-PCR revealed the presence of the respective mRNA. Only scattered cells contained nuclear immunoreactivity for glucocorticoid receptor as visualized by double immunostaining. Immunoreactive CBG could be recovered from the supernatant of cultures that had been exposed to 10(-5) M cortisol. Our observations indicate the endogenous expression of CBG in 1321N1 cells which may occur independently from classical glucocorticoid receptor pathways. Cortisol seems to facilitate liberation of CBG in a paracrine manner, perhaps through membrane action of the steroid. Effects of adrenal steroids on proliferation and apoptosis of certain glial tumors may in part depend on these mechanisms. PMID:19172388

Pusch, Larissa; Wegmann, Sonja; Caldwell, Jack D; Jirikowski, Gustav F

2009-06-01

194

Response of transcortin and alpha 2u-globulin to turpentine-induced inflammation in the rat: influence of corticosteroids and prolactin.  

Science.gov (United States)

Evidence is presented that (transcortin and alpha 2u-globulin react as negative acute-phase proteins in the rat. Thirty-six hours after turpentine injection, the serum concentration of these proteins showed a two- to threefold decrease. Thereafter, transcortin rapidly returned to normal values, whereas alpha 2u-globulin remained low. This reaction pattern was still present after adrenalectomy, adrenalectomy and administration of glucocorticoids, and after treatment with bromocriptine, a suppressor of prolactin secretion. It is concluded that changes in the secretion of glucocorticoids and prolactin are not required for the observed turpentine-induced decrease of transcortin and alpha 2u-globulin. PMID:6196428

Faict, D; Vandoren, G; De Moor, P; Lesaffre, E; Verhoeven, G

1983-12-01

195

NCI-Black-Reiter (NBR) male rats fail to develop renal disease following exposure to agents that induce alpha-2u-globulin (alpha2u) nephropathy  

OpenAIRE

The NCI-Black-Reiter (NBR) rat is the only strain of male rat known not to synthesize the hepatic form of the low molecular weight protein, ?2u-globulin. In previous studies, NBR rats were shown not to develop renal disease when exposed to decalin, a compound known to induce ?2u-globulin nephropathy in other rat strains. The objective of this study was to show that the presence of ?2u-globulin (?2u) is essential for the development of this syndrome in rats exposed to 2,2,4-trimethylpentan...

Dietrich, Daniel R.; Swenberg, James A.

1991-01-01

196

Globulin-platelet model predicts minimal fibrosis and cirrhosis in chronic hepatitis B virus infected patients  

Science.gov (United States)

AIM: To establish a simple model consisting of the routine laboratory variables to predict both minimal fibrosis and cirrhosis in chronic hepatitis B virus (HBV)-infected patients. METHODS: We retrospectively investigated 114 chronic HBV-infected patients who underwent liver biopsy in two different hospitals. Thirteen parameters were analyzed by step-wise regression analysis and correlation analysis. A new fibrosis index [globulin/platelet (GP) model] was developed, including globulin (GLOB) and platelet count (PLT). GP model = GLOB (g/mL) × 100/PLT (× 109/L). We evaluated the receiver operating characteristics analysis used to predict minimal fibrosis and compared six other available models. RESULTS: Thirteen clinical biochemical and hematological variables [sex, age, PLT, alanine aminotransferase, aspartate aminotransferase (AST), albumin, GLOB, total bilirubin (T.bil), direct bilirubin (D.bil), glutamyltransferase, alkaline phosphatase, HBV DNA and prothrombin time (PT)] were analyzed according to three stages of liver fibrosis (F0-F1, F2-F3 and F4). Bivariate Spearman’s rank correlation analysis showed that six variables, including age, PLT, T.bil, D.bil, GLOB and PT, were correlated with the three fibrosis stages (FS). Correlation coefficients were 0.23, -0.412, 0.208, 0.220, 0.314 and 0.212; and P value was 0.014, 0.05), the AUC of GP model was the largest among the seven models. CONCLUSION: By establishing a simple model using available laboratory variables, chronic HBV-infected patients with minimal fibrosis and cirrhosis can be diagnosed accurately, and the clinical application of this model may reduce the need for liver biopsy in HBV-infected patients. PMID:22719186

Liu, Xu-Dong; Wu, Jian-Lin; Liang, Jian; Zhang, Tao; Sheng, Qing-Shou

2012-01-01

197

Mathematical model for determination of colloid osmotic pressure: The role of albumin-globulin ratio  

Directory of Open Access Journals (Sweden)

Full Text Available BACKGROUND: Colloid Osmotic Pressure (COP is an important factor in the fluid balance of body compartments. COP is related to Total Protein (TP concentration and Albumin: Globulin Ratio (A/G. The A/G was not included in pervious empirical models, and therefore the main objective of this study was to develop a mathematical model to determine the COP in terms of TP concentration and A/G. METHODS: Sera with different A/G were prepared in-vitro, and COP was measured directly using colloid osmometer. The relationship between COP, TP concentration and A/G were determined mathematically. The validity of developed empirical models was confirmed by statistical comparison between measured and calculated COP in 122 serum samples obtained from hospitalized patients and healthy individuals. RESULTS: By non-linear regression, the following relationships were found between COP, TP concentration and A/G. All coefficients were statistically significant (p<0.05: COP = (4.0814 A/G TP/(A/G + 0.0153 TP; r2 = 0. 91272. COP = [5.3192 A/G -2.2252 (A/G2 + 0.2939 (A/G3] TP; r2 = 0.94737 No significant differences were indicated between measured COP and calculated one in clinical data. CONCLUSIONS: The variation of A/G may be the most effective factor for the differences between calculated and measured COP. This parameter must be considered when the direct measurement of COP is unavailable. KEY WORDS: Mathematical model, colloid osmotic pressure, oncotic pressure, albumin-globulin ratio.

Mehdi Nematbakhsh

2006-12-01

198

Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures  

OpenAIRE

Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L.) cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was signific...

Piotrowicz-cies?lak, Agnieszka I.; Michalczyk, Dariusz J.; Kamila Górska; Zofia Buli?ska-Radomska; Go?recki, Ryszard J.

2008-01-01

199

The reactive centre loop of corticosteroid-binding globulin (CBG) is a protease target for cortisol release.  

Science.gov (United States)

Corticosteroid-binding globulin (CBG) binds more than 90% of circulating cortisol and is a non-inhibitory member of the family of serine protease inhibitors (SERPINS) with an exposed elastase sensitive reactive centre loop (RCL). At sites of inflammation neutrophil activation can release elastase which may cleave the RCL and result in cortisol release from CBG. The RCL sequence also has two theoretical chymotrypsin cleavage sites and we used a monoclonal antibody with specificity for the RCL to investigate chymotrypsin cleavage of CBG. Here we show, for the first time, rapid chymotrypsin cleavage of the RCL of CBG, resulting in undetectable levels of intact CBG, whereas total CBG levels were unchanged. Coincident with both chymotrypsin and elastase cleavage there was an increase in the free cortisol fraction of serum to levels similar to when CBG had been inactivated by heat indicating total cortisol release from CBG. These findings demonstrate a new mechanism for cortisol release from its binding globulin. PMID:24424442

Lewis, John G; Elder, Peter A

2014-03-25

200

Intravenous immune globulins (IVIg) treatment for organizing pneumonia in a selective IgG immune deficiency state.  

Science.gov (United States)

We describe herein a 61-year-old woman who presented with fever, night sweats and cough. The diagnosis of pneumonia was established, but with symptom recurrence following antibiotic therapy, further diagnostics were performed. Biopsy via bronchoscopy revealed cryptogenic organizing pneumonia, and later on follow-up, a selective IgG immune deficiency was also diagnosed. Initial treatment of high-dose glucocorticoid therapy induced remission, but with dose reduction recurrence was observed. Intravenous immune globulin treatment was initiated and induced a successful clinical and radiological remission. Few cases of cryptogenic organizing pneumonia and hypogammaglobulinemia have been reported. To our knowledge, this is the fourth case described of cryptogenic organizing pneumonia with a hypogammaglobulinemia state and the first reported case of a selective immune deficiency state treated successfully with intravenous immune globulins. PMID:25391610

Gueta, Itai; Shoenfeld, Yehuda; Orbach, Hedi

2014-12-01

201

Effect of Anthrax Immune Globulin on Response to BioThrax (Anthrax Vaccine Adsorbed) in New Zealand White Rabbits  

Science.gov (United States)

Development of anthrax countermeasures that may be used concomitantly in a postexposure setting requires an understanding of the interaction between these products. Anthrax immune globulin intravenous (AIGIV) is a candidate immunotherapeutic that contains neutralizing antibodies against protective antigen (PA), a component of anthrax toxins. We evaluated the interaction between AIGIV and BioThrax (anthrax vaccine adsorbed) in rabbits. While pharmacokinetics of AIGIV were not altered by vaccination, the vaccine-induced immune response was abrogated in AIGIV-treated animals. PMID:23979740

Malkevich, Nina V.; Basu, Subhendu; Rudge, Thomas L.; Clement, Kristin H.; Chakrabarti, Ajoy C.; Aimes, Ronald T.; Nabors, Gary S.; Skiadopoulos, Mario H.

2013-01-01

202

Targeting of Natural Killer Cells by Rabbit Antithymocyte Globulin and Campath-1H: Similar Effects Independent of Specificity  

OpenAIRE

T cell depleting strategies are an integral part of immunosuppressive regimens widely used in the hematological and solid organ transplant setting. Although it is known to induce lymphocytopenia, little is known about the effects of the polyclonal rabbit antithymocyte globulin (rATG) or the monoclonal anti-CD52 antibody alemtuzumab on Natural Killer (NK) cells in detail. Here, we demonstrate that induction therapy with rATG following kidney/pancreas transplantation results in a rapid depletio...

Stauch, Diana; Dernier, Annelie; Sarmiento Marchese, Elizabeth; Kunert, Kristina; Volk, Hans-dieter; Pratschke, Johann; Kotsch, Katja

2009-01-01

203

Evaluation of various plastic microtiter plates with measles, toxoplasma, and gamma globulin antigens in enzyme-linked immunosorbent assays.  

OpenAIRE

Seventeen lots of microtiter plates which differed in lot, batch, plastic type, or manufacturer were evaluated as solid-phase carriers in enzyme-linked immunosorbent assays for antibodies to measles, toxoplasma, and human gamma globulin. Most plates of polystyrene or polyvinyl chloride were found to give acceptable binding. The final choice depended on the antigen to be attached. Variations in binding between lots, batches, and types of plastic were found. Well-to-well variation was found to ...

Shekarchi, I. C.; Sever, J. L.; Lee, Y. J.; Castellano, G.; Madden, D. L.

1984-01-01

204

Protein Pattern Analysis of Blood Globulin to Estimation of Genetic Distance among Population of Tegal, Magelang, and Mojosari Duck  

OpenAIRE

The research to estimation gene frequency on the protein pattern on blood globulin and to estimation genetic distance among population Tegal, Magelang and Mojosari duck. The material were blood samples from 30 heads of duck. Electroforesis technique was used Sodium Dedocyl Sulfate (SDS) polyacrilamide cel elekctroforesis according to Deutcher (1990) method with instrument of polyacrilamide electroforesis vertical system (Mini-protean II, Bio-Rad) was used for analysis protein of blood globul...

At, Sadewo

2005-01-01

205

The androgen-binding properties of sex hormone-binding globulin in male patients with liver cirrhosis  

OpenAIRE

Das Sexualhormon-bindende Globulin (SHBG) ist ein Serumprotein, das Testosteron (T) stärker bindet als 17-Beta-Estradiol (E2). Seine bei Leberzirrhose (Lci) erhöhten Serumspiegel tragen zu Hypogonadismus und Feminisierung männlicher Patienten bei. Voraussetzung ist eine intakte Steroidbindung. SHBG wurde aus 23 männlichen Lci-, 20 gesunden Männer-, 20 Hyperthyreose- und 19 Schwangerenseren durch Gelfiltration angereichert und mit einem Surface-Plasmon-Resonance-Biosensor analysiert. Es k...

Thaler, Markus Alexander

2008-01-01

206

Serious Infection Risk and Immune Recovery after Double Unit Cord Blood Transplantation without Anti-thymocyte Globulin  

OpenAIRE

Factors contributing to infection risk following cord blood transplantation (CBT) include the use of anti-thymocyte globulin (ATG), prolonged neutropenia, and failure to transfer immunity. To potentially reduce the infection risk we have investigated double unit CBT without ATG, and have evaluated the nature of serious infections in the first year after CBT using this approach. Seventy-two predominantly adult patients were transplanted for hematologic malignancies; 52 patients received myeloa...

Sauter, Craig; Abboud, Michelle; Jia, Xiaoyu; Heller, Glenn; Gonzales, Anne-marie; Lubin, Marissa; Hawke, Rebecca; Perales, Miguel-angel; Den Brink, Marcel R.; Giralt, Sergio; Papanicolaou, Genovefa; Scaradavou, Andromachi; Small, Trudy N.; Barker, Juliet N.

2011-01-01

207

Intravenous immune globulin use in patients with human immunodeficiency virus-related thrombocytopenia who require dental extraction.  

OpenAIRE

Five patients with human immunodeficiency virus (HIV)-related immune thrombocytopenia who were undergoing dental extraction were treated with intravenous immune globulin (IVIG). All patients received IVIG, 1 gram per kg, the day before the dental extraction and again the day of the dental extraction. Four patients had a previous history of minor clinical bleeding. The median baseline platelet count before extraction was 20 X 10(9) per liter (range 13 to 44). The median peak platelet count was...

Rarick, M. U.; Burian, P.; Guzman, N.; Espina, B.; Montgomery, T.; Jamin, D.; Levine, A. M.

1991-01-01

208

Retrospective diagnosis of Q fever in a country abattoir by the use of specific IgM globulin estimations  

Energy Technology Data Exchange (ETDEWEB)

Twenty-two cases of pyrexial illness which occurred amongst workers in a country abattoir were investigated retrospectively for Q fever, brucellosis, and leptospirosis. In 18, the illness was shown to be Q fever. No diagnoses were established for the other four. The demonstration of circulating Q-fever-specific IgM globulin was instrumental in establishing the diagnosis in many of the cases.

Murphy, A.M.; Hunt, J.G.

1981-10-03

209

Effect of Anthrax Immune Globulin on Response to BioThrax (Anthrax Vaccine Adsorbed) in New Zealand White Rabbits  

OpenAIRE

Development of anthrax countermeasures that may be used concomitantly in a postexposure setting requires an understanding of the interaction between these products. Anthrax immune globulin intravenous (AIGIV) is a candidate immunotherapeutic that contains neutralizing antibodies against protective antigen (PA), a component of anthrax toxins. We evaluated the interaction between AIGIV and BioThrax (anthrax vaccine adsorbed) in rabbits. While pharmacokinetics of AIGIV were not altered by vaccin...

Malkevich, Nina V.; Basu, Subhendu; Rudge, Thomas L.; Clement, Kristin H.; Chakrabarti, Ajoy C.; Aimes, Ronald T.; Nabors, Gary S.; Skiadopoulos, Mario H.; Ionin, Boris

2013-01-01

210

Fasting induces the generation of serum thyronine-binding globulin in Zucker rats  

International Nuclear Information System (INIS)

Five-month-old lean and obese Zucker rats were fasted for up to 7 days (lean rats) or 28 days (obese rats), and serum total and free T4 and T3 concentrations, percent free T4 and T3 by equilibrium dialysis, and the binding of [125I] T4 to serum proteins by gel electrophoresis were measured. In the lean rats, a 4- or 7-day fast resulted in significant decreases in serum total and free T4 and T3 concentrations. There was a decrease in the percent free T3 after 7 days of starvation. In contrast, a 4- or 7-day fast did not alter any of these variables in the obese rats. However, after 14 or more days of starvation, serum total T4 and T3 concentrations increased, and the percent free T4 and T3 decreased, resulting in no change in the serum free T4 or T3 concentrations in the obese rats. The percent of [125I]T4 bound to serum thyronine-binding globulin increased and the percent bound to thyronine-binding prealbumin decreased with the duration of the fast in both the lean and obese rats. The increase in serum thyronine-binding globulin binding of T4 can explain the increase in serum total T4 and T3 concentrations, the decrease in percent free T4 and T3, and the normal free hormone concentration in the long term fasted obese rats. The findings in the lean rats appear to be due to a combination of the known central hypothyroidism that occurs during 4-7 days of fasting and the fasting-induced changes in T4 binding in serum. Changes in T4 and T3 binding in in serum. Changes in T4 and T3 binding in serum during fasting in the rat must be considered when the effects of fasting on serum concentrations of the thyroid hormones, thyroid hormone kinetics, and the peripheral action of the thyroid hormones are evaluated

211

Proposal of abolition of the skin sensitivity test before equine rabies immune globulin application  

Directory of Open Access Journals (Sweden)

Full Text Available An epizootic outbreak of rabies occurred in 1995 in Ribeirão Preto, SP, with 58 cases of animal rabies (54 dogs, 3 cats and 1 bat confirmed by the Pasteur Institute of São Paulo, and one human death. The need to provide care to a large number of people for the application of equine rabies immune globulin (ERIG prevented the execution of the skin sensitivity test (SST and often also the execution of desensitization, procedures routinely used up to that time at the Emergency Unit of the University Hospital of the Faculty of Medicine of Ribeirão Preto, University of São Paulo (EU-UHFMRP-USP, a reference hospital for the application of heterologous sera. In view of our positive experience of several years with the abolition of SST and of the use of premedication before the application of antivenom sera, we used a similar schedule for ERIG application. Of the 1489 victims of animal bites, 1054 (71% received ERIG; no patient was submitted to SST and all received intravenously anti-histamines (anti-H1 + anti-H2 and corticosteroids before the procedure. The patients were kept under observation for 60 to 180 minutes and no adverse reaction was observed. On the basis of these results, since December 1995 ERIG application has been decentralized in Ribeirão Preto and has become the responsibility of the Emergency Unit of the University Hospital and the Central Basic Health Unit, where the same routine is used. Since then, 4216 patients have received ERIG (1818 at the Basic Health Unit and 2398 at the EU-UHFMRP, with no problems. The ideal would be the routine use of human rabies immune globulin (HRIG in public health programs, but this is problematic, because of their high cost. However, while this does not occur, the use of SST is no longer justified at the time of application of ERIG, in view of the clinical evidence of low predictive value and low sensitivity of SST involving the application of heterologous sera. It is very important to point out that a negative SST result may lead the health team to a feeling of false safety that no adverse reaction will occur, but this is not true for the anaphylactoid reactions. The decision to use premedication, which is based on knowledge about anaphylaxis and on the pharmacology of the medication used, is left to the judgment of health professionals, who should always be prepared for eventual untoward events.

CUPO Palmira

2001-01-01

212

Fasting induces the generation of serum thyronine-binding globulin in Zucker rats  

Energy Technology Data Exchange (ETDEWEB)

Five-month-old lean and obese Zucker rats were fasted for up to 7 days (lean rats) or 28 days (obese rats), and serum total and free T4 and T3 concentrations, percent free T4 and T3 by equilibrium dialysis, and the binding of (/sup 125/I) T4 to serum proteins by gel electrophoresis were measured. In the lean rats, a 4- or 7-day fast resulted in significant decreases in serum total and free T4 and T3 concentrations. There was a decrease in the percent free T3 after 7 days of starvation. In contrast, a 4- or 7-day fast did not alter any of these variables in the obese rats. However, after 14 or more days of starvation, serum total T4 and T3 concentrations increased, and the percent free T4 and T3 decreased, resulting in no change in the serum free T4 or T3 concentrations in the obese rats. The percent of (/sup 125/I)T4 bound to serum thyronine-binding globulin increased and the percent bound to thyronine-binding prealbumin decreased with the duration of the fast in both the lean and obese rats. The increase in serum thyronine-binding globulin binding of T4 can explain the increase in serum total T4 and T3 concentrations, the decrease in percent free T4 and T3, and the normal free hormone concentration in the long term fasted obese rats. The findings in the lean rats appear to be due to a combination of the known central hypothyroidism that occurs during 4-7 days of fasting and the fasting-induced changes in T4 binding in serum. Changes in T4 and T3 binding in serum during fasting in the rat must be considered when the effects of fasting on serum concentrations of the thyroid hormones, thyroid hormone kinetics, and the peripheral action of the thyroid hormones are evaluated.

Young, R.A.; Rajatanavin, R.; Moring, A.F.; Braverman, L.E.

1985-04-01

213

Yeast-recombinant hepatitis B vaccine: efficacy with hepatitis B immune globulin in prevention of perinatal hepatitis B virus transmission  

Energy Technology Data Exchange (ETDEWEB)

A yeast-recombinant hepatitis B vaccine was licensed recently by the Food and Drug administration and is now available. To assess the efficacy of the yeast-recombinant vaccine, the authors administered the vaccine in combination with hepatitis B immune globulin to high-risk newborns. If infants whose mothers were positive for both hepatitis B surface antigen and the e antigen receive no immunoprophylaxis, 70% to 90% become infected with the virus, and almost all become chronic carriers. Among infants in this study who received hepatitis B immune globulin at birth and three 5-/sup +/g doses of yeast-recombinant hepatitis B vaccine, only 4.8% became chronic carriers, a better than 90% level of protection and a rate that is comparable with that seen with immune globulin and plasma-derived hepatitis B vaccine. Hepatitis surface antigen and antibodies were detected by radioimmunoassay. These data suggest that, in this high-risk setting, the yeast-recombinant vaccine is as effective as the plasma-derived vaccine in preventing hepatitis B virus infection and the chronic carrier state.

Stevens, C.E.; Taylor, P.E.; Tong, M.J.; Toy, P.T.; Vyas, G.N.; Nair, P.V.; Weissman, J.Y.; Krugman, S.

1987-05-15

214

Yeast-recombinant hepatitis B vaccine: efficacy with hepatitis B immune globulin in prevention of perinatal hepatitis B virus transmission  

International Nuclear Information System (INIS)

A yeast-recombinant hepatitis B vaccine was licensed recently by the Food and Drug administration and is now available. To assess the efficacy of the yeast-recombinant vaccine, the authors administered the vaccine in combination with hepatitis B immune globulin to high-risk newborns. If infants whose mothers were positive for both hepatitis B surface antigen and the e antigen receive no immunoprophylaxis, 70% to 90% become infected with the virus, and almost all become chronic carriers. Among infants in this study who received hepatitis B immune globulin at birth and three 5-+g doses of yeast-recombinant hepatitis B vaccine, only 4.8% became chronic carriers, a better than 90% level of protection and a rate that is comparable with that seen with immune globulin and plasma-derived hepatitis B vaccine. Hepatitis surface antigen and antibodies were detected by radioimmunoassay. These data suggest that, in this high-risk setting, the yeast-recombinant vaccine is as effective as the plasma-derived vaccine in preventing hepatitis B virus infection and the chronic carrier state

215

Inhibition of Corticosteroid-Binding Globulin Gene Expression by Glucocorticoids Involves C/EBP?  

Science.gov (United States)

Corticosteroid-binding globulin (CBG), a negative acute phase protein produced primarily in the liver, is responsible for the transport of glucocorticoids (GCs). It also modulates the bioavailability of GCs, as only free or unbound steroids are biologically active. Fluctuations in CBG levels therefore can directly affect GC bioavailability. This study investigates the molecular mechanism whereby GCs inhibit the expression of CBG. GCs regulate gene expression via the glucocorticoid receptor (GR), which either directly binds to DNA or acts indirectly via tethering to other DNA-bound transcription factors. Although no GC-response elements (GRE) are present in the Cbg promoter, putative binding sites for C/EBP?, able to tether to the GR, as well as HNF3? involved in GR signaling, are present. C/EBP?, but not HNF3?, was identified as an important mediator of DEX-mediated inhibition of Cbg promoter activity by using specific deletion and mutant promoter reporter constructs of Cbg. Furthermore, knockdown of C/EBP? protein expression reduced DEX-induced repression of CBG mRNA, confirming C/EBP?’s involvement in GC-mediated CBG repression. Chromatin immunoprecipitation (ChIP) after DEX treatment indicated increased co-recruitment of C/EBP? and GR to the Cbg promoter, while C/EBP? knockdown prevented GR recruitment. Together, the results suggest that DEX repression of CBG involves tethering of the GR to C/EBP?. PMID:25335188

Verhoog, Nicolette; Allie-Reid, Fatima; Vanden Berghe, Wim; Smith, Carine; Haegeman, Guy; Hapgood, Janet; Louw, Ann

2014-01-01

216

Physicochemical and structural characterisation of protein isolate, globulin and albumin from soapnut seeds (Sapindus mukorossi Gaertn.).  

Science.gov (United States)

The amino acid (AA) composition and physicochemical and conformational properties of protein isolate (SNPI), globulin (SNG) and albumin (SNA) fractions from soapnut seeds were evaluated. The essential AA of SNG, SNA and SNPI (except sulfur-containing AA) are sufficient for the FAO/WHO suggested requirements for 2-5year old infants. SNG and SNPI showed similar electrophoresis patterns and AA compositions, the subunit of those proteins consisted of two polypeptides linked by disulfide bonds. In contrast, SNA showed a different AA compositions and SDS-PAGE pattern. Both SNG and SNPI presented a typical U-shape protein solubility (PS)-pH profile, SNA showed a completely different PS-pH profile, especially at pH 2.0-4.0. The near-UV circular dichroism (CD), differential scanning calorimetry (DSC) and tryptophan fluorescence spectra analyses indicated that the flexibility in tertiary conformations decreased in the order: SNA>SNPI>SNG, while soapnut proteins had a similar secondary conformation, with a highly ordered structure (the ?-types), as evidenced by far-UV CD spectra. PMID:25212151

Yin, Shou-Wei; Chen, Ji-Cheng; Sun, Shang-De; Tang, Chuan-He; Yang, Xiao-Quan; Wen, Qi-Biao; Qi, Jun-Ru

2011-09-15

217

Treatment of acute rejection of cadaveric renal allografts with rabbit antithymocyte globulin.  

Science.gov (United States)

In a prospective randomized single-blind trial, we compared the effectiveness of rabbit antithymocyte globulin (RATG) in the treatment of acute renal graft rejection with the results of treatment by high oral doses of prednisone. Twenty recipients of cadaveric kidneys were included in each group. In the RATG group, the prednisone dose was not increased and a dose-by-rosette protocol was used to keep T cell levels between 50 and 150/mm3. In this group 15 of the 20 patients responded to the treatment. One of these patients lost her kidney afterward because of a technical failure. In five patients rejection was irreversible despite a subsequent course of high-dose prednisone orally. In the prednisone group, 13 patients showed a good response, but 3 of them only after a subsequent course of RATG. The remaining seven patients underwent nephrectomy before a course of RATG could be given. One patient in this group died of septicemia. In either group there were six second rejection episodes, but they developed 2.2 months later in the RATG group. All second rejection episodes were treated with the alternative regimen and all patients responded to this treatment. Renal function after 6 months was similar in both groups. Less infections occurred in the RATG group. Prior to rejection, there were no differences in concentrations of peripheral T cells between both groups. Treatment of acute rejections with RATG is an effective and safe procedure which is steroid sparing. PMID:7039017

Hoitsma, A J; Reekers, P; Kreeftenberg, J G; van Lier, H J; Capel, P J; Koene, R A

1982-01-01

218

A multicenter trial of antithymocyte globulin in aplastic anemia and related diseases.  

Science.gov (United States)

One hundred fifty patients with bone marrow failure were treated in three groups with antithymocyte globulin (ATG; Upjohn, Kalamazoo, MI) in a multicenter trial. Patients were assessed at 3, 6, and 12 months after initiation of treatment by three criteria: transfusion independence, clinical improvement, and blood counts. Group I consisted of 77 patients with acute severe aplastic anemia, randomized to receive either ten or 28 days of ATG. There was no significant difference between the two arms of this protocol: 47% of all patients were clinically improved and 31% were transfusion independent at 3 months. Of the severely affected patients, 27% died before 3 months; most deaths occurred early in treatment. Factors associated with survival in severely affected patients included male sex, age less than 40 years, absolute neutrophil count greater than 200/microL, and idiopathic etiology. Neutrophil counts generally increased by 8 weeks after treatment, but patients continued to show improvement to 1 year posttreatment. In Group II, 44 patients with moderate or chronic severe aplastic anemia were randomized to receive either ten days of ATG or 3 months of high-dose nandrolone decanoate. No patient initially treated with androgens recovered, but 28% of ATG-treated cases achieved transfusion independence at 3 months. Group III consisted of patients with a variety of bone marrow failure syndromes. Patients with pancytopenia and cellular bone marrow showed response rates similar to those of patients with chronic or moderate aplastic anemia. PMID:3058228

Young, N; Griffith, P; Brittain, E; Elfenbein, G; Gardner, F; Huang, A; Harmon, D; Hewlett, J; Fay, J; Mangan, K

1988-12-01

219

Clonality of acquired primary pure red cell aplasia: effectiveness of antithymocyte globulin.  

Science.gov (United States)

Primary pure red cell aplasia (PRCA) was diagnosed in two male patients, 65 and 69 years old respectively. In both, surface markers of peripheral blood nuclear cells revealed the presence of TCR alphabeta+ phenotype. Clonality of T cells was confirmed by the polymerase chain reaction in both patients, in whom, prednisone at a dose of 1 mg/kg/day improved the anemia and lower doses caused its renewal, resulting in the reappearance of the patient's transfusion requirement. On the other hand, the anemia seems to have been treated permanently (second case) with horse antithymocyte globulin (ATG) (20 mg/kg/day 1 to 8 +) since his hemoglobin was about 15 g/dl at the time of writing. In the first patient, the hemoglobin level was 10.5 g/dl one month after the administration of ATG (15 mg/kg/d 1 to 5 +), but unfortunately, the patient died because of a massive gastrointestinal bleeding on the fortieth day following this treatment. We, therefore, suggest that, patients with acquired primary PRCA should be screened to detect the presence of a T-cell clone and recommend that, treatment should start earlier with ATG, if the PRCA is due to a T-cell clonal disorder. PMID:11911428

Dinçol, G; Aktan, M; Nalçaci, M; Yavuz, A S; Keskin, H; Dawson, B; Dinçol, K

2001-01-01

220

Hyporesponsiveness to glucocorticoids in mice genetically deficient for the corticosteroid binding globulin  

DEFF Research Database (Denmark)

Corticosteroid binding globulin (CBG) is the carrier for glucocorticoids in plasma. The protein is believed to keep the steroids inactive and to regulate the amount of free hormone acting on target tissues (free hormone hypothesis). Here, we generated a mouse model genetically deficient for CBG to test the contribution of the carrier to glucocorticoid action and adrenocortical stress response. The absence of CBG resulted in a lack of corticosterone binding activity in serum and in an approximately 10-fold increase in free corticosterone levels in CBG-null mice, consistent with its role in regulation of circulating free hormone levels. Surprisingly, cbg(-/-) animals did not exhibit features seen in organisms with enhanced glucocorticoid signaling. Rather, the mice exhibited increased activity of the pituitary axis of hormonal control, normal levels of gluconeogenetic enzymes, and fatigue, as well as an aggravated response to septic shock, indicating an inability to appropriately respond to the excess free corticosterone in the absence of CBG. Thus, our data suggest an active role for CBG in bioavailability, local delivery, and/or cellular signal transduction of glucocorticoids that extends beyond a function as a mere cargo transporter.

Petersen, Helle Heibroch; Andreassen, Thomas K

2006-01-01

221

Role of corticosteroid binding globulin in the fast actions of glucocorticoids on the brain.  

Science.gov (United States)

Corticosteroid binding globulin (CBG) is a glycoprotein synthesized in liver and secreted in the blood where it binds with a high affinity but low capacity glucocorticoid hormones, cortisol in humans and corticosterone in laboratory rodents. In mammals, 95% of circulating glucocorticoids are bound to either CBG (80%) or albumin (15%) and only the 5% free fraction is able to enter the brain. During stress, the concentration of glucocorticoids rises significantly and the free fraction increases even more because CBG becomes saturated. However, glucocorticoids unbound to CBG are cleared from the blood more quickly. Our studies on mice totally devoid of CBG (Cbg k.o.) showed that during stress these mutant mice display a lower rise of glucocorticoids than the wild-type controls associated with altered emotional reactivity. These data suggested that CBG played a role in the fast actions of glucocorticoids on behavior. Further analyses demonstrated that stress-induced memory retrieval impairment, an example of the fast action of glucocorticoids on the brain is abolished in the Cbg k.o. mice. This effect of stress on memory retrieval could be restored in the Cbg k.o. mice by infusing corticosterone directly in the hippocampus. The mechanisms explaining these effects involved an increased clearance but no difference in corticosterone production. Thus, CBG seems to have an important role in maintaining in blood a glucocorticoid pool that will be able to access the brain for the fast effects of glucocorticoids. PMID:24252379

Moisan, M P; Minni, A M; Dominguez, G; Helbling, J C; Foury, A; Henkous, N; Dorey, R; Béracochéa, D

2014-03-01

222

Treatment of aplastic anemia with antithymocyte globulin, high-dose corticosteroids, and androgens.  

Science.gov (United States)

A total of 46 patients with aplastic anemia (34 severe; 12 moderate) were treated with antihuman thymocyte globulin (ATG), high-dose methylprednisolone, and oxymetholone. Early symptoms of ATG toxicity included fever, rash, and bronchospasm. Signs of serum sickness also developed in 23 patients. Complications associated with high doses of steroids were hyperglycemia, hypertension, fluid retention, gastrointestinal hemorrhage, and aseptic necrosis of the hip. Other morbidity possible associated with steroid administration included seizures, arrhythmias, and headache with papilledema. Studies of elevated liver function necessitated discontinuation of androgen therapy in eight patients. A complete or partial hematological response was noted in 19 patients (41%). Of these, three have had recurrent cytopenias, of whom one has developed a myelodysplastic syndrome. There are currently 34 patients surviving, and 12 who have died. Actuarial survival at three years is 65%. These response and survival data are comparable to those of previous trials using ATG and androgens without high-dose steroids. A prospective, randomized trial is needed to determine whether the addition of high-dose corticosteroids to ATG does significantly increase the rate and frequency of response in order to justify the toxicity of this additional immunosuppressive therapy in the treatment of aplastic anemia. PMID:3493172

Doney, K; Storb, R; Buckner, C D; McGuffin, R; Witherspoon, R; Deeg, H J; Appelbaum, F R; Sullivan, K M; Thomas, E D

1987-03-01

223

The effect of equine antithymocyte globulin on the outcomes of reduced intensity conditioning for AML.  

Science.gov (United States)

Whether or not the benefits of antithymocyte globulin (ATG) on engraftment and GVHD are offset by increased risk of relapse, delayed T-cell recovery and increased infections remains controversial. We retrospectively studied the effect of ATG in 144 AML patients, 34 of whom received ATG, undergoing reduced intensity conditioning (RIC) umbilical cord blood transplantation (UCB) or HLA-matched sibling PBSC. ATG patients had not received intensive chemotherapy for 3 months before transplantation for UCB, 6 months for PBSC. There were no differences in engraftment between ATG and non-ATG patients. The cumulative incidences of TRM as well as acute and chronic GVHD in ATG-treated patients were not statistically different. ATG patients had significantly more infections between 46 and 180 days post transplantation. Unexpectedly, after adjusting for donor type, relapse was lower among ATG recipients (relative risk (RR) 0.5, 95% confidence interval (CI) 0.3-1.0, P=0.04). In summary, administration of ATG to AML patients undergoing RIC had no adverse impact on major clinical outcomes. ATG may be indicated for patients at higher risk of graft failure after allogeneic hematopoietic cell transplantation (allo-HCT). PMID:25243623

Hagen, P; Wagner, J E; DeFor, T E; Dolan, M; Arora, M; Warlick, E; Weisdorf, D; Brunstein, C G

2014-12-01

224

A randomized controlled trial of daclizumab versus anti-thymocyte globulin induction for heart transplantation  

Science.gov (United States)

Background The purpose of this study was to test the efficacy and safety of daclizumab (DZM) versus anti-thymocyte globulin (ATG) as a component of induction therapy in heart transplant recipients. Methods Thirty heart transplant patients were randomized to receive either ATG or DZM during induction therapy. Patients in the DZM group received an initial dose of 2 mg/kg intravenous (IV) at the time of transplant and 1 mg/kg IV on postoperative day 4. Discussion Recipient, donor, and intraoperative variables did not differ significantly between groups. The cost of induction therapy, total drug cost, and hospital ward costs were significantly less for the DZM group. Average absolute lymphocyte and platelet counts were significantly higher in the DZM group. There were no significant differences in the incidence of rejection, infection, malignancy, or steroid-induced diabetes. One year survival was excellent in both groups (87%, P?=?0.1). Daclizumab is a safe component of induction therapy in heart transplantation. PMID:25093077

2014-01-01

225

Prolonged cyclosporine administration after antithymocyte globulin delays but does not prevent relapse in severe aplastic anemia.  

Science.gov (United States)

In severe aplastic anemia, approximately one-third of responders to standard horse antithymocyte globulin (h-ATG) plus cyclosporine (CsA) will relapse. Anecdotal experience has suggested that a gradual CsA taper might avoid relapse, but this practice has not been rigorously assessed prospectively. In 2003, we adopted a strategy to taper CsA beyond 6 months, with the intention to reduce hematologic relapse compared with our extensive historical experience. In total, 102 patients received h-ATG/CsA for 6 months in two sequential clinical protocols: 67 patients (66%) responded and all had the CsA dose tapered per protocol over the subsequent 18 months (total of 2 years). The rate of relapse at 5 years was 33% (95% CI 27-44%), which did not differ from our large historical relapse experience (patients treated before 2003) of 30-40%, in protocols in which CsA was simply discontinued at 6 months. However, time to relapse was prolonged by about 1 year with the longer CsA course. The rates of clonal evolution and overall survival did not differ between the two cohorts. We infer from this large prospective study that CsA taper as implemented delayed but did not prevent relapse. The kinetics of relapse with long course CsA does suggest that a lower long-term dose might be adequate to maintain patients in remission. PMID:24971433

Scheinberg, Phillip; Rios, Olga; Scheinberg, Priscila; Weinstein, Barbara; Wu, Colin O; Young, Neal S

2014-06-01

226

Radioimmunoassay of thyroxine-binding globulin: evaluation of a kit and diagnostic application  

International Nuclear Information System (INIS)

Serum thyroxine-binding globulin (TBG) was measured by using a radioimmunoassay method. The within-batch of variation (CV) of the assay was 5% or less at different TBG concentrations with a between-batch variation of less than 10%. The mean TBG concentration in hypothyroidism (28.5 plus or minus 6.7 mg/l) was significantly higher, and that in hyperthyroidism (22.3 plus or minus 3.5 mg/l) was significantly lower, than the euthyroid mean (25.4 plus or minus 6.9 mg/l). Acutely ill patients suffering from various non-thyroid illnesses were found to have significantly low mean TBG (13.8 plus or minus 4.3 mg/l). A group of women in late pregnancy had a higher mean value, as expected. Serum TBG level, however, was ineffective as a thyroid function test because of overlapping concentrations between groups. The derived index T4:TBG ratio was found to be superior to free thyroxine index (FTI) in many cases of acutely ill patients, pregnancy and hereditary TBG abnormality. The 95% confidence limit for the ratio in euthyroids, was 2.3 to 7.1. Serum TBG level with T4:TBG ratio is recommended as a replacement for the T3-uptake test and FTI. (author)

227

Potential Role of Tumor Necrosis Factor-? in Downregulating Sex Hormone–Binding Globulin  

Science.gov (United States)

Low plasma sex hormone–binding globulin (SHBG) levels are associated with obesity and predict the development of type 2 diabetes. The reason why obese individuals have low circulating SHBG has been attributed to hyperinsulinemia, but no mechanistic evidence has been described. The aim of the current study is to explore whether tumor necrosis factor-? (TNF-?) rather than insulin could be the main factor accounting for low SHBG levels in obesity. We performed in vitro and in vivo studies using human HepG2 cells and human SHBG transgenic mice. In addition, a cross-sectional study to explore the relationship between TNF-? and SHBG in obese patients and an interventional study to examine the effect of insulin administration on circulating SHBG in type 2 diabetic patients were performed. We provide evidence that TNF-?, but not insulin, is the main factor by which SHBG is reduced in obesity. Plasma SHBG was significantly increased rather than decreased after insulin treatment in diabetic patients. TNF-?–induced reduction of SHBG expression was mediated by downregulating HNF4A. Finally, a negative and independent correlation was found between plasma TNF-? receptor 1 and SHBG levels in obese patients. Our results suggest that TNF-? plays an important role downregulating SHBG in chronic low-grade inflammatory diseases such as obesity and type 2 diabetes. PMID:22210320

Simó, Rafael; Barbosa-Desongles, Anna; Lecube, Albert; Hernandez, Cristina; Selva, David M.

2012-01-01

228

Anti-Cryptococcal-Globulin-Latex Production for Rapid Detection of Cryptococcus neoformans Polysaccharide Antigen in Cryptococcosis  

Directory of Open Access Journals (Sweden)

Full Text Available Cryptococcosis has become the fourth leading life-threatening opportunistic infection in patients with AIDS, but also occurs in non-AIDS patients. In view of the increasing numbers of infection during last decade in Iran, use of rapid, sensitive and specific test for diagnosis of cryptococcal disease has become important than ever. We aimed to produce the reagents for latex cryptococcal antigen test. The antigen was prepared from NCPF 3168 strain of Cryptococcus neoformans. Anticapsular antiserum of C. neoformans raised in rabbits and latex carboxylate- modified beads were coated with antiserum. The maximally- reactive globulin dilution was obtained at dilution of 1:400. For evaluation of efficacy of reagents, challenged 38 BALB/C mice and other 38 mice were used as controls. The mice were bled and autopsied. Brain, heart and lung were checked by direct, histopathological and cultural examination for cryptococcosis. The sera from case and control mice were tested with Immunomycologic (Immy kit and also our produced reagents (OPR for detection of cryptococcal antigen. Moreover, 15 cerebrospinal fluid and 15 serum samples from patients with cryptococcal meningitis, 30 with aspergillosis, 30 with suspected other fungal infections, and 30 from healthy individuals were tested as well. The results showed that the sensitivity (97.3% and specificity (100% of OPR was quite comparable with those of Immy kit . Therefore, it could be regarded as a substitute for commercial kits.

F Zaini

2005-09-01

229

Clinical evaluation of thyroxine-binding globulin (TBG) as a marker of liver tumors  

Energy Technology Data Exchange (ETDEWEB)

This investigation was undertaken to evaluate thyroxine-binding globulin (TGB) as a marker of liver tumors, in conjection with the liver scintigram. Of 30 patients with primary hepatocellular carcinoma (PHC), 22 (73.3%) showed significantly higher TBG concentrations. Eight patients (26.7%) showed normal TBG concentrations. In the case of 27 our of 30 patients with definite liver tumors, defects were apparent on the scintigrams. But seven of them had normal TBG concentrations in spite of the defects on the scintigrams. Out of 33 postoperative patients with liver metastasis, 28 (84%) had a raised TBG concentration. Only five (15.2%) had a normal TBG level. In 31 patients (93.9%) out of 33 with liver metastasis, a definite diagnosis was made on the basis of the liver scintigram. In 28 (90.3%) of these 31 people, the TBG concentration was higher than normal. Among 63 patients with liver tumors, both primary and metastatic, the test sensitivity for liver tumors was 92.1% (58/63) based on the accuracy of the liver scintigram. It was 79.4% (50/63) based on the TBG measurement. Why TBG increases to such an extent in spite of the euthyroid state remains unexplained. But it may be concluded that elevated TBG with positive liver scintigram furnishes a sensitive, fairly reliable, nonspecific tumor marker to determine liver tumors, especially in the case of liver metastasis.

Terui, S.

1984-03-01

230

Serum Thyroxine to Thyroxine-Binding Globulin Ratio in Pregnancy and Newborn  

Energy Technology Data Exchange (ETDEWEB)

To evaluate the diagnostic value of the ratio of serum thyroxine(T{sub 4}) /thyroxine-binding globulin (TBG) for the thyroid status in pregnancy and newborn serum thyroxine, TBG, triiodothyronine, and free thyroxine levels were radioimmunoassayed in normal pregnant women at each of the trimesters, and the calculated serum T{sub 4}/TBG ratios were compared with other parameters such as T{sub 3}/TBG ratio and free T{sub 4}/TBG ratio and free T{sub 4}/TBG ratio. Serum T{sub 4} levels were elevated with the proportionate increase in TBG levels during pregnancy, leading to the nearly constant value of serum T{sub 4}/TBG ratios as in normal non-pregnant controls. In contrast, serum T{sub 3}/TBG and free T{sub 4}/TBG ratios varied considerably during pregnancy. In newborn, T{sub 4} levels were nearly not changed with compared non-pregnant control value and TBG levels were elevated. The results indicate that serum T{sub 4}/TBG ratio is a better parameter than others in evaluating the thyroid status during pregnancy and but newborn is a no better.

Kim, Ji Yeul [Chonnam University School of Medicine, Kwangju (Korea, Republic of)

1982-09-15

231

Histopathologic features of transplant glomerulopathy associated with response to therapy with intravenous immune globulin and rituximab.  

Science.gov (United States)

Transplant glomerulopathy (TG) is associated with poor long-term allograft survival and is often accompanied by microcirculation inflammation. Histopathologic scoring may inform prognosis and help guide therapy. We retrospectively assessed 33 patients with biopsy-proven TG. All biopsies were given a glomerulitis (g) and peritubular capillaritis (ptc) score. We determined allograft survival and serum creatinine stability in three different score groups: g < 2 and ? 2, ptc < 2 and ? 2, and (g + ptc) < 4 and ? 4. We assessed the impact of treatment with intravenous immune globulin (IVIG) and rituximab on outcomes. Graft survival and serum creatinine stability did not differ in each of the histopathologic score groups. Higher-score groups were associated with the presence of concomitant antibody-mediated rejection and were more likely to receive IVIG and rituximab. Treatment with IVIG and rituximab resulted in stability of serum creatinine within the higher-score groups, but not in the lower-score groups. Stabilization of serum creatinine was associated with an improvement in donor-specific antibody. Histopathologic scoring in kidney allograft biopsies with TG may help guide treatment. The combination of IVIG and rituximab appears to be beneficial in patients whose biopsies have moderate or severe microvascular injury. PMID:24579925

Kahwaji, Joseph; Najjar, Reiad; Kancherla, Deepika; Villicana, Rafael; Peng, Alice; Jordan, Stanley; Vo, Ashley; Haas, Mark

2014-05-01

232

Simultaneous determination of free thyroxine and capacity of thyroxine-binding globulin  

Energy Technology Data Exchange (ETDEWEB)

A simple method is described for the simultaneous determination of capacity thyroxine-binding of globulin (TBG) and free thyroxine concentration (FT/sub 4/). The ratio of bound to free T/sub 4/ (B/F) is first determined for two total-T/sub 4/ concentrations using a Sephadex G-25 competitive-binding technique. TBG capacity and FT/sub 4/ can both be calculated assuming a known value of affinity constant of TBG. The method is linear over a twenty-fold serum dilution. FT/sub 4/ calculated is identical to that calculated using the method of Irvine. TBG capacity is shown to be linearly correlated to TBG concentration as determined by radioimmunoassay and is consistent with a molecular weight of 69,000 Daltons and one T/sub 4/ binding site per molecule. FT/sub 4/ is found to correlate with the free thyroxine index in a complicated way, depending on the degree of TBG saturation.

Harpen, M.D.; Lee, W.N.P.; Siegel, J.A.; Greenfield, M.A.

1981-03-01

233

Induction with Rabbit Antithymocyte Globulin following Orthotopic Liver Transplantation for Hepatitis C  

Directory of Open Access Journals (Sweden)

Full Text Available Background: Hepatitis C (HCV is the most common indication for liver transplantation in the US. Objective: Since steroids are the major stimulus of viral replication, we postulated that steroid-free immu-nosuppression might be a safer approach. Methods: From January 1995 to October 2002, we used steroid plus calcineurin inhibitor (CNI immuno-suppression after liver transplantation for HCV (steroid group, n=81. From October 2002 to June 2007, rabbit antithymocyte globulin (RATG induction, followed by CNI and azathioprine (RATG group, n=73 was utilized. Results: There were no differences in 1- and 3-year patient/allograft survival rates. The incidence of acute rejection rate (19% vs. 28%, of biopsy-proven HCV recurrence (70% vs. 75%, and chronic rejection (6% vs. 9% were comparable. The mean time to develop recurrent HCV was significantly longer in the RATG group (16.2 vs. 9.2 months, p=0.008. The incidence of severe portal fibrosis appears to be lower in RATG group compared to the steroid group; 14% vs. 4% (p=0.07. Conclusions: RATG induction is safe and effective after liver transplantation for HCV, but has no impact on the incidence of HCV recurrence and patient/allograft survival. However, a significant delay in time to HCV recurrence and a trend toward less rejection and portal fibrosis was observed.

R. F. Saidi

2011-10-01

234

Serum sex steroids, gonadotrophins and sex hormone-binding globulin in prostatic hyperplasia  

Directory of Open Access Journals (Sweden)

Full Text Available Background: Benign prostatic hyperplasia (BPH develops in elderly males when serum androgens are relatively lower than in healthy younger males, but it is not well understood whether and how sex steroids are altered in prostatic hyperplasia. It is also uncertain whether there is any change in sex steroid levels in males older than 40 years of age. The use of androgens in elderly males is often discouraged because of the probable worsening effect of androgens on prostatism. This study aimed to determine the relationship between prostatic hyperplasia and sex steroid levels and whether there is any significant change in these hormones after the age of 40 years. Subjects and Methods: We studied healthy males of age 240 years with (n=92 or without (n=93 clinical prostatic hyperplasia. Serum testosterone, estradiol, gonadotrophins and sex hormone-binding globulin (SHBG were compared. The hormones and SHBG were also correlated with age. Results: No significant difference was found in any hormone in cases with prostatic hyperplasia as compared with the controls. There was no significant age-related change in any hormone except estradiol where as a negative correlation (P< .003 with age was found. Conclusions: Serum sex steroids and SHBG remained unchanged in symptomatic prostatic hyperplasia and except for estradiol there was no significant age-related change in serum testosterone, gonadotrophins and SHBG in healthy males after the fourth decade. More studies are needed to confirm the age-related decline of estrogens in males.

Ansari Mohammad Abdul

2008-01-01

235

Localization of sex hormone binding globulin in the rat vomeronasal organ.  

Science.gov (United States)

Volatile and non-volatile derivates of gonadal steroids are known to act as pheromones in many mammalian species. Pheromones have multiple effects on the brain via the olfactory system. Their primary port of entry seems to be the vomeronasal organ (VNO) but the underlying cellular and molecular mechanisms are unclear so far. Recently we localized sex hormone binding globulin (SHBG) in both the main and the accessory olfactory system of rat with immunocytochemistry and RT-PCR. The accessory olfactory system consisting of VNO and accessory olfactory bulb showed high expression of SHBG. In the present paper we studied SHBG expression in the VNO in greater detail. In semithin sections we found SHBG immunostaining in the perinuclear cytoplasm of some of the sensory neurons, in sensory cilia and in their axons. A portion of the basal cells and some of the goblet cells in the non-sensory epithelium showed intense SHBG staining. SHBG was abundant in exocrine cells of the vomeronasal glands, perhaps compartimentalized in secretory vesicles. In situ hybridization revealed specific signals in sensory and non-sensory cells of the VNO. Our findings indicate that SHBG expressed in the VNO may be liberated into nasal secretions to bind aerosolic steroids. SHBG in sensory cells may be involved in signaling actions of pheromones. PMID:25154024

Ploß, V M; Gebhart, V M; Gisder, D; Dölz, W; Jirikowski, G F

2014-11-01

236

Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes  

DEFF Research Database (Denmark)

Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (?20%), pauci-mannosidic (?40%), and complex forms (?40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants ?-1,2-xylose and ?-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectincontaining two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2 (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has the potential to serve as a model system for studying the role of seed proteins and their glycosylation in food allergy.

Dam, Svend Secher; Thaysen-Andersen, Morten

2013-01-01

237

XML Storage  

OpenAIRE

XML documents require new techniques for compact storage and efficient query processing. In this entry, we classify the main approaches taken to store XML documents by industrial systems and in research works.

Barbosa, Denilson; Bohannon, Phil; Freire, Juliana; Kanne, Carl-christian; Manolescu, Ioana; Vassalos, Vasilis; Yoshikawa, Masatoshi

2009-01-01

238

Comparative study regarding the association of alpha-2U globulin with the nephrotoxic mechanism of certain petroleum-based Air Force fuels. Final report, 1 September 1984-31 August 1986  

Energy Technology Data Exchange (ETDEWEB)

Fischer 344 male rats have a dose- and time-dependent proximal tubular degeneration induced by certain petroleum-based fuels. This degeneration may be associated with a low molecular weight alpha globulin (termed alpha-2U globulin). A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion-exchange and hydroxylapatite chromatography. Rocket immunoelectrophoretic and isoelectric focusing techniques were developed to quantitatively and qualitatively assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds.

Eurell, T.E.

1986-10-31

239

Globulin-platelet model predicts minimal fibrosis and cirrhosis in chronic hepatitis B virus infected patients  

Directory of Open Access Journals (Sweden)

Full Text Available AIM: To establish a simple model consisting of the routine laboratory variables to predict both minimal fibrosis and cirrhosis in chronic hepatitis B virus (HBV-infected patients. METHODS: We retrospectively investigated 114 chronic HBV-infected patients who underwent liver biopsy in two different hospitals. Thirteen parameters were analyzed by step-wise regression analysis and correlation analysis. A new fibrosis index [globulin/platelet (GP model] was developed, including globulin (GLOB and platelet count (PLT. GP model = GLOB (g/mL × 100/PLT (× 109/L. We evaluated the receiver operating characteristics analysis used to predict minimal fibrosis and compared six other available models. RESULTS: Thirteen clinical biochemical and hematological variables [sex, age, PLT, alanine aminotransferase, aspartate aminotransferase (AST, albumin, GLOB, total bilirubin (T.bil, direct bilirubin (D.bil, glutamyltransferase, alkaline phosphatase, HBV DNA and prothrombin time (PT] were analyzed according to three stages of liver fibrosis (F0-F1, F2-F3 and F4. Bivariate Spearman’s rank correlation analysis showed that six variables, including age, PLT, T.bil, D.bil, GLOB and PT, were correlated with the three fibrosis stages (FS. Correlation coefficients were 0.23, -0.412, 0.208, 0.220, 0.314 and 0.212; and P value was 0.014, < 0.001, 0.026, 0.018, 0.001 and 0.024, respectively. Univariate analysis revealed that only PLT and GLOB were significantly different in the three FS (PLT: F = 11.772, P < 0.001; GLOB: F = 6.612, P = 0.002. Step-wise multiple regression analysis showed that PLT and GLOB were also independently correlated with FS (R2 = 0.237. By Spearman’s rank correlation analysis, GP model was significantly correlated with the three FS (r = 0.466, P < 0.001. The median values in F0-F1, F2-F3 and F4 were 1.461, 1.720 and 2.634. Compared with the six available models (fibrosis index, AST-platelet ratio, FIB-4, fibrosis-cirrhosis index and age-AST model and age-PLT ratio, GP model showed a highest correlation coefficient. The sensitivity and positive predictive value at a cutoff value < 1.68 for predicting minimal fibrosis F0-F1 were 72.4% and 71.2%, respectively. The specificity and negative predictive value at a cutoff value < 2.53 for the prediction of cirrhosis were 84.5% and 96.7%. The area under the curve (AUC of GP model for predicting minimal fibrosis and cirrhosis was 0.762 [95% confidence interval (CI: 0.676-0.848] and 0.781 (95% CI: 0.638-0.924. Although the differences were not statistically significant between GP model and the other models (P all > 0.05, the AUC of GP model was the largest among the seven models. CONCLUSION: By establishing a simple model using available laboratory variables, chronic HBV-infected patients with minimal fibrosis and cirrhosis can be diagnosed accurately, and the clinical application of this model may reduce the need for liver biopsy in HBV-infected patients.

Xu-Dong Liu

2012-01-01

240

Effects of corticosterone pellets on baseline and stress-induced corticosterone and corticosteroid-binding-globulin.  

Science.gov (United States)

Exogenous administration of glucocorticoids is a widely used and efficient tool to investigate the effects of elevated concentrations of these hormones in field studies. Because the effects of corticosterone are dose and duration-dependent, the exact course of plasma corticosterone levels after exogenous administration needs to be known. We tested the performance of self-degradable corticosterone pellets (implanted under the skin) in elevating plasma corticosterone levels. We monitored baseline (sampled within 3min after capture) total corticosterone levels and investigated potential interactions with corticosteroid-binding-globulin (CBG) capacity and the endogenous corticosterone response to handling in Eurasian kestrel Falco tinnunculus and barn owl Tyto alba nestlings. Corticosterone pellets designed for a 7-day-release in rodents elevated circulating baseline total corticosterone during only 2-3 days compared to placebo-nestlings. Highest levels occurred 1-2days after implantation and levels decreased strongly thereafter. CBG capacity was also increased, resulting in a smaller, but still significant, increase in baseline free corticosterone levels. The release of endogenous corticosterone as a response to handling was strong in placebo-nestlings, but absent 2 and 8 days after corticosterone pellet implantation. This indicates a potential shut-down of the hypothalamo-pituitary-adrenal axis after the 2-3 days of elevated baseline corticosterone levels. 20 days after pellet implantation, the endogenous corticosterone response to handling of nestlings implanted with corticosterone pellets attained similar levels as in placebo-nestlings. Self-degradable pellets proved to be an efficient tool to artificially elevate circulating baseline corticosterone especially in field studies, requiring only one intervention. The resulting peak-like elevation of circulating corticosterone, the concomitant elevation of CBG capacity, and the absence of an endogenous corticosterone response to an acute stressor have to be taken into account. PMID:18996387

Müller, Claudia; Almasi, Bettina; Roulin, Alexandre; Breuner, Creagh W; Jenni-Eiermann, Susanne; Jenni, Lukas

2009-01-01

241

New directions for rabbit antithymocyte globulin (Thymoglobulin(®)) in solid organ transplants, stem cell transplants and autoimmunity.  

Science.gov (United States)

In the 30 years since the rabbit antithymocyte globulin (rATG) Thymoglobulin(®) was first licensed, its use in solid organ transplantation and hematology has expanded progressively. Although the evidence base is incomplete, specific roles for rATG in organ transplant recipients using contemporary dosing strategies are now relatively well-identified. The addition of rATG induction to a standard triple or dual regimen reduces acute cellular rejection, and possibly humoral rejection. It is an appropriate first choice in patients with moderate or high immunological risk, and may be used in low-risk patients receiving a calcineurin inhibitor (CNI)-sparing regimen from time of transplant, or if early steroid withdrawal is planned. Kidney transplant patients at risk of delayed graft function may also benefit from the use of rATG to facilitate delayed CNI introduction. In hematopoietic stem cell transplantation, rATG has become an important component of conventional myeloablative conditioning regimens, following demonstration of reduced acute and chronic graft-versus-host disease. More recently, a role for rATG has also been established in reduced-intensity conditioning regimens. In autoimmunity, rATG contributes to the treatment of severe aplastic anemia, and has been incorporated in autograft projects for the management of conditions such as multiple sclerosis, Crohn's disease, and systemic sclerosis. Finally, research is underway for the induction of tolerance exploiting the ability of rATG to induce immunosuppresive cells such as regulatory T-cells. Despite its long history, rATG remains a key component of the immunosuppressive armamentarium, and its complex immunological properties indicate that its use will expand to a wider range of disease conditions in the future. PMID:25164240

Mohty, Mohamad; Bacigalupo, Andrea; Saliba, Faouzi; Zuckermann, Andreas; Morelon, Emmanuel; Lebranchu, Yvon

2014-09-01

242

Purification and properties of squirrel monkey (Saimiri sciureus) corticosteroid binding globulins  

International Nuclear Information System (INIS)

Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, the authors found the behavior of CBG in squirrel monkey (Saimiri sciureus) serum during fractionation by polyacrylamide gel electrophoresis or Sephadex chromatography was consistent with a molecule about twice the size of that found in most species. To more fully understand the basis for this difference, they purified the protein by sequential affinity and DEAE-Sepharose chromatographies. The final product was obtained in greater than 60% yield and was found to migrate as a single homogeneous band when examined by electrophoresis. The steroid binding specificity of the purified protein was identical with that of the protein in the starting serum. In contrast to the single protein band observed following electrophoresis under normal conditions, separations in the presence of sodium dodecyl sulfate (SDS) resolved the pure protein into two bands: one at 54,000 daltons and one at 57,000 daltons. Unlike other species, squirrel monkey CBG exists as a dimer in its native state. Antibodies were generated against the purified material and tested for cross-reactivity against the sera from other species by both radioimmunodiffusion and radioimmunoassay techniques. Only serum from titi monkeys was observed to cross-reactm titi monkeys was observed to cross-react when examined by radioimmunoassay. Taken together, the results suggest that New World monkey CBG's are distinct from those of other species in both size and immunologic characteristics

243

Interactions of thyroxine with thyroxine-binding globulin of low binding capacity  

Energy Technology Data Exchange (ETDEWEB)

The physico-chemical interactions between thyroxine (T/sub 4/) and thyroxine-binding globulin (TBG) of low binding capacity were investigated by radioimmunoassay, equilibrium dialysis, and reverse flow electrophoresis. Knowledge of total and free T/sub 4/ (FT/sub 4/) concentrations in serum, and of the total binding capacity of the protein carrier (TTBG), allowed the determination of the association constant ruling these interactions (K/sub tbg/). Correlation between T/sub 4/ and FT/sub 4/ varies with TTBG, shifting the normal range for T/sub 4/. The level of FT/sub 4/ in serum is a function of the fractional saturation of TBG by endogenous hormones, which depends on T/sub 4/ and TTBG. Data on T/sub 4/, TTBG, and K/sub tbg/ were integrated into the general equation of the law of mass action and the results showed a very significant linear correlation with the values of FT/sub 4/ measured by equilibrium dialysis. It is concluded that the misleading results of serum T/sub 4/ measurements and of the free T/sub 4/ index, obtained in euthyroid individuals with low TTBG, cannot be ascribed to a reduction of the intrinsic sensitivity of the assay due to oversaturation of TBG by endogenous T/sub 4/, as previously postulated by others, but to a shift of their normal ranges produced by abnormal variations of TTBG. These results stress the need for data regarding TTBG for the proper interpretation of T/sub 4/, and for the calculation of the fractional saturation of TBG and the FT/sub 4/ concentration in serum. The authors have solved this problem by using an empirical equation relating TTBG to T/sub 4/ and triiodothyronine (T/sub 3/) uptake, which was previously derived by other workers.

Cuaron, A.

1987-09-01

244

Low-dose rabbit anti-thymoglobin globulin versus basiliximab for induction therapy in kidney transplantation.  

Science.gov (United States)

We conducted a single-center prospective double-arm open-labeled study on kidney transplant patients from 2010 to 2011 to evaluate the efficacy of induction therapy using low, single-dose rabbit-antithymocyte globulin (r-ATG), 1.5 mg/kg on Day 0 (n = 80, 60 males, mean age 35.9 years), versus basiliximab (Interleukin-2 blocker) 20 mg on Days 0 and 4 (n = 20, 12 males, mean age 45.1 years) on renal allograft function in terms of serum creatinine (SCr), rejection and infection episodes and patient/graft survival and cost. Demographic and post-transplant follow-up including immunosuppression was similar in both groups. In the r-ATG group, donors were unrelated (spouse, n = 25), deceased (n = 31) and parents/siblings (others), with a mean HLA match of 1.58. Donors in the basiliximab group were living unrelated (spouse, n = 15) and deceased (n = 5), with a mean HLA match of 1.56. No patient/graft was lost in the r-ATG group over a mean of one year follow-up, and the mean SCr was 1.28 mg/dL with 7.5% acute rejection (AR) episodes; infections were also not observed. In the basiliximab group, over the same period of follow-up, there was 95% death-censored graft survival, and the mean SCr was 1.23 mg/dL with 10% AR episodes. One patient died due to bacterial pneumonia and one succumbed to coronary artery disease; one graft was lost due to uncontrolled acute humoral and cellular rejection. The cost of r-ATG and basiliximab were $600 and $2500, respectively. We conclude that induction immunosuppressive therapy with a low-dose r-ATG may be a better option as compared with basiliximab in terms of graft function, survival and cost benefit in kidney transplant patients. PMID:24969194

Patel, Himanshu V; Kute, Vivek B; Vanikar, Aruna V; Shah, Pankaj R; Gumber, Manoj R; Engineer, Divyesh P; Trivedi, Hargovind L

2014-07-01

245

Timing of rabbit antithymocyte globulin induction therapy in kidney transplantation: an observational cohort study  

Science.gov (United States)

Background Literature on the timing of rabbit antithymocyte globulin (rATG) induction and its effects on kidney transplant outcomes is limited. The manufacturer recommends that the first dose be given intra-operatively, however this may present clinical practice risks and challenges. Our objective was to assess the impact of the timing of the first dose of rATG on kidney transplant outcomes. Methods Incident kidney transplant recipients (KTR) from January 2002 to December 2009 receiving the first dose of rATG post-operatively (Post, n?=?353) or before reperfusion (Pre, n?=?124) were evaluated. Outcomes assessed included eGFR at 1-year, delta eGFR (12 versus 1 month), and incidence of biopsy-proven acute rejection, graft loss, death, and a composite of the time-to-event outcomes. The impact of timing on outcomes was adjusted for potential confounders and assessed using linear and Cox regression models. Results Among 435 KTR surviving with function to 12 months post-transplant, there was no significant difference in mean estimated glomerular filtration rate or eGFR (55.0 versus 56.7 mL/min, P?=?0.46) and delta eGFR (1.8 versus 0.3 mL/min, P?=?0.40) in Post versus Pre groups, respectively. At a median follow-up of 3 years, the composite endpoint (time to first biopsy-proven acute rejection, graft loss, or death) was similar by timing group (adjusted HR?=?0.94; 95% CI: 0.58, 1.53, P?=?0.81) in the total study population. Conclusions Timing of rATG had no appreciable impact on clinically relevant endpoints in this study cohort. These results support consideration of more flexible timing of the first dose of rATG induction in KTR. PMID:24387192

2014-01-01

246

Outcomes of immunosuppressant therapy with lower dose of antithymocyte globulin and cyclosporine in aplastic anemia.  

Science.gov (United States)

Objective Immunosuppressant therapy (IST) with antithymocyte globulin (ATG) and cyclosporine is an established treatment option for patients with aplastic anemia (AA), who are not eligible for allogeneic stem cell transplantation. However, data on the dose of ATG and its efficacy from the developing countries is minimal. Methods We performed a retrospective analysis of all AA patients (age >12 years), treated with equine ATG and cyclosporine from a single center in India. Patients who received or were eligible for stem cell transplantation were excluded. The overall response rate (ORR) to IST was calculated at 3 and 6 months. We also determined the influence of using a lower dose of Atgam ATG (25 mg/kg/day × 4 days) and compared its efficacy against the standard dose of locally manufactured Thymogam ATG (40 mg/kg/day × 4 days). Factors influencing the ORR were analyzed using Fisher's exact test with a significant P < 0.05. Results Thirty-nine patients with AA treated with ATG and cyclosporine were studied. Median age was 31 years with a male:female ratio of 0.85:1. The ORR was 58% at 3 months, 77% at 6 months and was similar with lower dose Atgam and standard dose Thymogam. On multivariate analysis of ORR at 6 months, the interval between the onset of symptoms to the initiation of therapy was close to attaining statistical significance (odds ratio 23.53, P value 0.053) while the other variables did not attain significance. Conclusions IST with equine ATG in a lower dose (25 mg/kg/day × 4 days) and cyclosporine is a feasible and effective treatment option for AA in resource-constrained settings. PMID:25181305

Malhotra, Pankaj; Bodh, Vijay; Guru Murthy, Guru Subramanian; Datta, Anup Kumar; Varma, Neelam; Varma, Subhash

2014-09-01

247

Thyroxine binding to serum thyronine-binding globulin in thyroidectomized adult and normal neonatal rats  

Energy Technology Data Exchange (ETDEWEB)

The amount of tracer (125I)T4 bound to serum thyronine-binding globulin (TBG) was measured by polyacrylamide gel electrophoresis in adult thyroidectomized (TX) rats and normal 1-day to 4-week-old rat puts. Thyroidectomy was associated with the appearance of significant amounts of (125I)T4 binding to serum TBG in lean rats, but not in obese Zucker rats. Treatment of the TX rats in vivo with replacement doses of T4 prevented this increase in TBG binding, but enrichment of serum from TX rats with T4 did not. Significant amounts of tracer (125I)T4 binding to TBG was present in serum from 1- to 3-week-old normal rat pups, but not in 1-day- or 4-week-old pups. There were significantly higher levels of TBG binding of (125I)T4 in serum from 2-week-old rat pups raised in litters of 16 pups compared to those raised in litters of 4 pups. All manipulations that result in the appearance of TBG in rat serum also result in either weight loss or a slowing in the rate of growth, suggesting that the appearance of TBG in rat serum has a nutritional component. This possibility is further supported by the observations that increases in TBG binding of (125I)T4 are not found in obese Zucker rats fed a low protein-high carbohydrate diet for 14 days or fasted for 7 days, or after thyroidectomy, perhaps owing to the large stores of fuel in the obese rat.

Young, R.A.; Meyers, B.; Alex, S.; Fang, S.L.; Braverman, L.E.

1988-05-01

248

Associations between sex hormone binding globulin and metabolic syndrome parameters in premenopausal obese women  

Directory of Open Access Journals (Sweden)

Full Text Available Background and Aims: The aim of this study was to determine sex hormone binding globulin (SHBG concentrations in premenopausal obese women and to evaluate the relationships between sex hormones and features of the metabolic syndrome (MetS. Settings and Design: Retrospective cross-sectional analysis was carried out on 350 obese patients aged 25 to 69 years referred to the Department of Endocrinology, Pamukkale University in 2002-2003. Materials and Methods: 125 premenopausal euthyroid patients were eligible for this study. Subjects were divided into two groups according to the body mass index (BMI: Group I, women with BMI < 30 kg/m 2 (n = 17 and Group II,, women with BMI ? 30 kg/m 2 (n = 108. Median SHBG concentration of Group I was 50.1 nmol/L. Group II was divided into two subgroups according to the median SHBG concentration of Group I: subjects with high SHBG levels (SHBG concentration ? median level of the control group, i.e ? 50.1 nmol/L and subjects with low SHBG levels (< 50.1 nmol/L. All statistical analyses were performed using SPSS 9.0 software (SPSS Inc.. Results: No significant difference was found in mean age between the low and high SHBG groups. The low SHBG group was significantly heavier, and with higher waist circumference than the high SHBG group. In the low SHBG group, fasting glucose, postprandial glucose and gamma glutamyl transferase (GGT and free androgen index (FAI were significantly higher. Lipid profile, blood pressure, uric acid, insulin and HOMA were found similar between two groups. Linear regression analyses revealed that body mass index and FAI were significant, being independent predictors of SHBG concentrations in premenopausal women. (r = 0.365, r square = 0.134. Conclusions: It is concluded that low SHBG concentrations may indicate visceral obesity and glucose intolerance in premenopausal women.

Akin Fulya

2008-10-01

249

Pseudomonas aeruginosa elastase disrupts the cortisol-binding activity of corticosteroid-binding globulin.  

Science.gov (United States)

The serine protease inhibitor (SERPIN) family member corticosteroid-binding globulin (CBG) is the main carrier of glucocorticoids in plasma. Human CBG mediates the targeted release of cortisol at sites of inflammation through cleavage of its reactive center loop (RCL) by neutrophil elastase. The RCLs of SERPIN family members are targeted by diverse endogenous and exogenous proteases, including several bacterial proteases. We tested different bacteria for their ability to secrete proteases that disrupt CBG cortisol-binding activity, and characterized the responsible protease and site of CBG cleavage. Serum CBG integrity was assessed by Western blotting and cortisol-binding capacity assay. Effects of time, pH, temperature, and protease inhibitors were tested. Proteolytically active proteins from bacterial media were purified by fast protein liquid chromatography, and the active protease and CBG cleavage sites were identified by mass spectrometry. Among the bacteria tested, medium from Pseudomonas aeruginosa actively disrupted the cortisol-binding activity of CBG. This proteolytic activity was inhibited by zinc chelators and occurred most efficiently at pH 7 and elevated physiological temperature (ie, 41°C). Mass spectrometric analysis of a semi-purified fraction of P. aeruginosa media identified the virulence factor LasB as the responsible protease, and this was confirmed by assaying media from LasB-deficient P. aeruginosa. This metalloprotease cleaves the CBG RCL at a major site, distinct from that targeted by neutrophil elastase. Our results suggest that humoral responses to P. aeruginosa infection are influenced by this pathogen's ability to secrete a protease that promotes the release of the anti-inflammatory steroid, cortisol, from its plasma transport protein. PMID:24848868

Simard, Marc; Hill, Lesley A; Underhill, Caroline M; Keller, Bernd O; Villanueva, Ivan; Hancock, Robert E W; Hammond, Geoffrey L

2014-08-01

250

Thyroxine binding to serum thyronine-binding globulin in thyroidectomized adult and normal neonatal rats  

International Nuclear Information System (INIS)

The amount of tracer [125I]T4 bound to serum thyronine-binding globulin (TBG) was measured by polyacrylamide gel electrophoresis in adult thyroidectomized (TX) rats and normal 1-day to 4-week-old rat puts. Thyroidectomy was associated with the appearance of significant amounts of [125I]T4 binding to serum TBG in lean rats, but not in obese Zucker rats. Treatment of the TX rats in vivo with replacement doses of T4 prevented this increase in TBG binding, but enrichment of serum from TX rats with T4 did not. Significant amounts of tracer [125I]T4 binding to TBG was present in serum from 1- to 3-week-old normal rat pups, but not in 1-day- or 4-week-old pups. There were significantly higher levels of TBG binding of [125I]T4 in serum from 2-week-old rat pups raised in litters of 16 pups compared to those raised in litters of 4 pups. All manipulations that result in the appearance of TBG in rat serum also result in either weight loss or a slowing in the rate of growth, suggesting that the appearance of TBG in rat serum has a nutritional component. This possibility is further supported by the observations that increases in TBG binding of [125I]T4 are not found in obese Zucker rats fed a low protein-high carbohydrate diet for 14 days or fasted for 7 days, or after thyroidectomy, perhaps owing to the large stores of fuel in the obese rat

251

Intravenous immune globulin in hereditary inclusion body myopathy: a pilot study  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Hereditary Inclusion Body Myopathy (HIBM is an autosomal recessive, adult onset, non-inflammatory neuromuscular disorder with no effective treatment. The causative gene, GNE, codes for UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, which catalyzes the first two reactions in the synthesis of sialic acid. Reduced sialylation of muscle glycoproteins, such as ?-dystroglycan and neural cell adhesion molecule (NCAM, has been reported in HIBM. Methods We treated 4 HIBM patients with intravenous immune globulin (IVIG, in order to provide sialic acid, because IgG contains 8 ?mol of sialic acid/g. IVIG was infused as a loading dose of 1 g/kg on two consecutive days followed by 3 doses of 400 mg/kg at weekly intervals. Results For all four patients, mean quadriceps strength improved from 19.0 kg at baseline to 23.2 kg (+22% directly after IVIG loading to 25.6 kg (+35% at the end of the study. Mean shoulder strength improved from 4.1 kg at baseline to 5.9 kg (+44% directly after IVIG loading to 6.0 kg (+46% at the end of the study. The composite improvement for 8 other muscle groups was 5% after the initial loading and 19% by the end of the study. Esophageal motility and lingual strength improved in the patients with abnormal barium swallows. Objective measures of functional improvement gave variable results, but the patients experienced improvements in daily activities that they considered clinically significant. Immunohistochemical staining and immunoblotting of muscle biopsies for ?-dystroglycan and NCAM did not provide consistent evidence for increased sialylation after IVIG treatment. Side effects were limited to transient headaches and vomiting. Conclusion The mild benefits in muscle strength experienced by HIBM patients after IVIG treatment may be related to the provision of sialic acid supplied by IVIG. Other sources of sialic acid are being explored as treatment options for HIBM.

Dorward Heidi

2007-01-01

252

Proteome profiling of seed storage proteins reveals the nutritional potential of Salicornia brachiata Roxb., an extreme halophyte.  

Science.gov (United States)

Salicornia brachiata is an extreme halophyte that grows in salty marshes and is considered to be a potential alternative crop for seawater agriculture. Salicornia seeds are rich in protein, and its tender shoots are eaten as salad greens. Seed storage proteins were fractionated by sequential extraction using different solvents, including distilled water for albumins, NaCl (1.0 M) for globulins, NaOH (0.1 N) for glutelins, and ethanol (70% v/v) for prolamins. Globulins accounted for 54.75% of the total seed storage proteins followed by albumins (34.30%) and glutelins (8.70%). The fractionated proteins were characterized using 2D-diagonal SDS-PAGE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. The globulin fraction, composed of seven intermolecular disulfide-linked polypeptide pairs of molecular mass 63.5, 62.5, 54.7, 53.0, 43.2, 38.5, and 35.1 kDa, encompassed a basic and an acidic subunit. Two-dimensional gels revealed approximately 32 spots, with isoelectric points and molecular masses ranging from 4.93 to 11.6 and from ?5.2 to ?109.4 kDa, respectively. Protein spots were identified by MALDI-TOF MS peptide mass fingerprint analysis and further classified. Homology analysis demonstrated that 19% of the proteins were involved in metabolism, 16% were involved in signaling, and 15% were regulatory proteins. Peptide mass fingerprint analysis confirmed the presence of inter- and intramolecular disulfide linkages in the globulin fraction. Sulfur-rich proteins are of high nutritional value, and disulfides make S. brachiata a potential source of dietary supplementation. PMID:22494338

Jha, Bhavanath; Singh, Nater Pal; Mishra, Avinash

2012-05-01

253

Expression of biologically active human corticosteroid binding globulin by insect cells: acquisition of function requires glycosylation and transport.  

OpenAIRE

Human corticosteroid binding globulin (hCBG) is a 50- to 55-kDa serum glycoprotein that binds cortisol and progesterone with high affinity. To map the steroid-binding domain and to investigate the folding pathways of hCBG, we have established an expression system based on infection of insect cells with a recombinant baculovirus encoding hCBG. Infected Spodoptera frugiperda (Sf9) cells secrete immunoreactive hCBG at high levels (16-24 pmol per 10(6) cells per 40 h), and the recombinant protein...

Ghose-dastidar, J.; Ross, J. B.; Green, R.

1991-01-01

254

Oxytocin and/or steroid hormone binding globulin infused into the ventral tegmental area modulates progestogen-mediated lordosis  

OpenAIRE

Estradiol (E2) and progesterone (P4) have classical, steroid receptor-mediated actions in the ventral medial hypothalamus to initiate lordosis of female rodents. P4 and the P4 metabolite and neurosteroid, 5?-pregnan-3?-ol-20-one (3?,5?-THP), have non-classical actions in the midbrain ventral tegmental area (VTA) to modulate lordosis. We investigated the role of steroid hormone binding globulin (SHBG) and oxytocin in the VTA as mechanisms for these effects. Rats were ovariectomized and sur...

Frye, Cheryl A.; Walf, Alicia A.

2009-01-01

255

Apparent effect of immune serum globulin prophylaxis in the military on viral hepatitis incidence in the civilian population in Israel.  

OpenAIRE

Since 1969, extensive use of immune serum globulin in the Israel Defence Force for prophylaxis against hepatitis A virus (HAV) infection has produced a sharp decline in the incidence of the disease. However, it is not clear whether this policy has affected the susceptibility of Israeli adults to HAV infection. In this study, we examined the effect of the immunisation policy on the incidence of hepatitis A virus infection in the civilian population in the 15-44 year age group, which includes a...

Green, M. S.; Block, C.

1989-01-01

256

Storage Rings  

International Nuclear Information System (INIS)

Storage rings are circular machines that store particle beams at a constant energy. Beams are stored in rings without acceleration for a number of reasons (Tab. 1). Storage rings are used in high-energy, nuclear, atomic, and molecular physics, as well as for experiments in chemistry, material and life sciences. Parameters for storage rings such as particle species, energy, beam intensity, beam size, and store time vary widely depending on the application. The beam must be injected into a storage ring but may not be extracted (Fig. 1). Accelerator rings such as synchrotrons are used as storage rings before and after acceleration. Particles stored in rings include electrons and positrons; muons; protons and anti-protons; neutrons; light and heavy, positive and negative, atomic ions of various charge states; molecular and cluster ions, and neutral polar molecules. Spin polarized beams of electrons, positrons, and protons were stored. The kinetic energy of the stored particles ranges from 10-6 eV to 3.5 x 1012 eV (LHC, 7 x 1012 eV planned), the number of stored particles from one (ESR) to 1015 (ISR). To store beam in rings requires bending (dipoles) and transverse focusing (quadrupoles). Higher order multipoles are used to correct chromatic aberrations, to suppress instabilities, and to compensate for nonlinear field errors of dipoles and quadrupoles. Magnetic multipole functions can be combined in magnets. Beams are stored bunched with ra magnets. Beams are stored bunched with radio frequency systems, and unbunched. The magnetic lattice and radio frequency system are designed to ensure the stability of transverse and longitudinal motion. New technologies allow for better storage rings. With strong focusing the beam pipe dimensions became much smaller than previously possible. For a given circumference superconducting magnets make higher energies possible, and superconducting radio frequency systems allow for efficient replenishment of synchrotron radiation losses of large current electron or positron beams. Storage rings have instrumentation to monitor the electrical and mechanical systems, and the beam quality. Computers are used to control the operation. Large storage rings have millions of control points from all systems. The time dependent beam intensity I(t) can often be approximated by an exponential function I(t) = I(0) exp(-t/?) (1) where the decay time ? and, correspondingly, the store time ranges from a few turns to 10 days (ISR). ? can be dominated by a variety of effects including lattice nonlinearities, beam-beam, space charge, intrabeam and Touschek scattering, interaction with the residual gas or target, or the lifetime of the stored particle. In this case, the beam lifetime measurement itself can be the purpose of a storage ring experiment. The main consideration in the design of a storage ring is the preservation of the beam quality over the store length. The beam size and momentum spread can be reduced through cooling, often leading to an increase in the store time. For long store times vacuum considerations are important since the interaction rate of the stored particles with the residual gas molecules is proportional to the pressure, and an ultra-high vacuum system may be needed. Distributed pumping with warm activated NEG surfaces or cold surfaces in machines with superconducting magnets are ways to provide large pumping speeds and achieve low pressures even under conditions with dynamic gas loads. The largest application of storage rings today are synchrotron light sources, of which about 50 exist world wide. In experiments where the beam collides with an internal target or another beam, a storage ring allows to re-use the accelerated beam many times if the interaction with the target is sufficiently small. In hadron collider and ion storage rings store times of many hours or even days are realized, corresponding to up to 1011 turns and thereby target passages. Ref. (3) is the first proposal for a collider storage ring. A number of storage rings exist where the beam itself or its decay products are

257

Storage Rings  

Energy Technology Data Exchange (ETDEWEB)

Storage rings are circular machines that store particle beams at a constant energy. Beams are stored in rings without acceleration for a number of reasons (Tab. 1). Storage rings are used in high-energy, nuclear, atomic, and molecular physics, as well as for experiments in chemistry, material and life sciences. Parameters for storage rings such as particle species, energy, beam intensity, beam size, and store time vary widely depending on the application. The beam must be injected into a storage ring but may not be extracted (Fig. 1). Accelerator rings such as synchrotrons are used as storage rings before and after acceleration. Particles stored in rings include electrons and positrons; muons; protons and anti-protons; neutrons; light and heavy, positive and negative, atomic ions of various charge states; molecular and cluster ions, and neutral polar molecules. Spin polarized beams of electrons, positrons, and protons were stored. The kinetic energy of the stored particles ranges from 10{sup -6} eV to 3.5 x 10{sup 12} eV (LHC, 7 x 10{sup 12} eV planned), the number of stored particles from one (ESR) to 1015 (ISR). To store beam in rings requires bending (dipoles) and transverse focusing (quadrupoles). Higher order multipoles are used to correct chromatic aberrations, to suppress instabilities, and to compensate for nonlinear field errors of dipoles and quadrupoles. Magnetic multipole functions can be combined in magnets. Beams are stored bunched with radio frequency systems, and unbunched. The magnetic lattice and radio frequency system are designed to ensure the stability of transverse and longitudinal motion. New technologies allow for better storage rings. With strong focusing the beam pipe dimensions became much smaller than previously possible. For a given circumference superconducting magnets make higher energies possible, and superconducting radio frequency systems allow for efficient replenishment of synchrotron radiation losses of large current electron or positron beams. Storage rings have instrumentation to monitor the electrical and mechanical systems, and the beam quality. Computers are used to control the operation. Large storage rings have millions of control points from all systems. The time dependent beam intensity I(t) can often be approximated by an exponential function I(t) = I(0) exp(-t/{tau}) (1) where the decay time {tau} and, correspondingly, the store time ranges from a few turns to 10 days (ISR). {tau} can be dominated by a variety of effects including lattice nonlinearities, beam-beam, space charge, intrabeam and Touschek scattering, interaction with the residual gas or target, or the lifetime of the stored particle. In this case, the beam lifetime measurement itself can be the purpose of a storage ring experiment. The main consideration in the design of a storage ring is the preservation of the beam quality over the store length. The beam size and momentum spread can be reduced through cooling, often leading to an increase in the store time. For long store times vacuum considerations are important since the interaction rate of the stored particles with the residual gas molecules is proportional to the pressure, and an ultra-high vacuum system may be needed. Distributed pumping with warm activated NEG surfaces or cold surfaces in machines with superconducting magnets are ways to provide large pumping speeds and achieve low pressures even under conditions with dynamic gas loads. The largest application of storage rings today are synchrotron light sources, of which about 50 exist world wide. In experiments where the beam collides with an internal target or another beam, a storage ring allows to re-use the accelerated beam many times if the interaction with the target is sufficiently small. In hadron collider and ion storage rings store times of many hours or even days are realized, corresponding to up to 1011 turns and thereby target passages. Ref. [3] is the first proposal for a collider storage ring. A number of storage rings exist where the beam itself or its decay products are the object of s

Fischer, W.

2011-01-01

258

Data Storage  

Science.gov (United States)

Even as home computers are being equipped with hard drives more massive than most users need, the scientific community is facing challenges of inadequate data storage systems. Experiments conducted in research facilities can produce unimaginable amounts of information, and computer scientists are working on ways to handle and manage it.Virtually every modern computer system incorporates several different storage technologies to process data efficiently. A gentle introduction to registers, caches, and other forms of memory is given on this site (1). A more advanced description of computer architecture and memory hierarchy is offered by Sun Microsystems (2). The paper explains the importance of having small, fast caches in the microprocessor to improve performance and reduce the delay of accessing the large, slow hard drive. Colossal Storage (3) is a company specializing in a new method of holographic data storage. While current technologies are hindered by area density, the proposed technology will expand into three dimensions and use the disk's volume to write data. Although the company's homepage is a bit poorly organized, some interesting insights into the technology and several white papers are available. InPhase Technologies is another company exploring holographic storage; however, its goal is to create a device that could be the successor to the DVD. In this article (4), the potential capabilities and specifications of such a device are discussed. A working prototype has already been demonstrated, paving the way for future commercially viable drives based on the technology. A short paper by the Department of Energy Office of Science (5) describes the impending rush of data generated by future scientific applications. It outlines the obstacles that must be overcome, including data mobility, extraction and analysis, and storage hardware. Possibly the most ambitious storage system ever created will be used to capture data from the world's most advanced particle physics facility. The European Organisation for Nuclear Research (CERN) will begin operating its Large Hadron Collider in 2007. The system that will collect and manage data from this instrument is described on this page (6). Documentation about the architecture and operation of the CERN Advanced STORage Manager (CASTOR) is provided. A news article from January 2003 (7) describes a new hard drive that can fit gigabytes of data in a tiny space. Said to measure about an inch wide, the Microdrive will be used in portable multimedia devices like digital video cameras. The second part of the article tells of the IBM Millipede project, which uses nanotechnology to make storage devices with less area than a dime. Satellites that monitor the Earth's environment have provided NASA with over a petabyte (a million gigabytes) of data. This article (8) discusses this remarkable achievement and how the system allows constant access to all of the information.

Leske, Cavin.

259

Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK) kernel globulin Caracterizaçao funcional das globulinas de amêndoa de castanha-do-Pará após a acetilação  

OpenAIRE

Defatted Brazil nut kernel flour, a rich source of high quality proteins, is presently being utilized in the formulation of animal feeds. One of the possible ways to improve its utilization for human consumption is through improvement in its functional properties. In the present study, changes in some of the functional properties of Brazil nut kernel globulin were evaluated after acetylation at 58.6, 66.2 and 75.3% levels. The solubility of acetylated globulin was improved above pH 6.0 but wa...

Cíntia Maria Pinto Ramos; Pushkar Singh Bora

2004-01-01

260

ETL storage-ring FEL  

International Nuclear Information System (INIS)

Oscillation of a free electron laser (FEL) has been achieved on the electron storage ring TERAS of Electrotechnical Laboratory (ETL) at 598 nm in Mar. 1991, which was the first visible FEL in Japan. In the present paper, the oscillation experiment, and the progress after the first oscillation are described. (author)

261

Role of corticosteroid binding globulin in emotional reactivity sex differences in mice.  

Science.gov (United States)

Sex differences exist for stress reactivity as well as for the prevalence of depression, which is more frequent in women of reproductive age and often precipitated by stressful events. In animals, the differential effect of stress on male's and female's emotional behavior has been well documented. Crosstalk between the gonadal and stress hormones, in particular between estrogens and glucocorticoids, underlie these sex differences on stress vulnerability. We have previously shown that corticosteroid binding globulin (CBG) deficiency in a mouse model (Cbg k.o.) leads, in males, to an increased despair-like behavior caused by suboptimal corticosterone stress response. Because CBG displays a sexual dimorphism and is regulated by estrogens, we have now investigated whether it plays a role in the sex differences observed for emotional reactivity in mice. By analyzing Cbg k.o. and wild-type (WT) animals of both sexes, we detected sex differences in despair-like behavior in WT mice but not in Cbg k.o. animals. We showed through ovariectomy and estradiol (E2) replacement that E2 levels explain the sex differences found in WT animals. However, the manipulation of E2 levels did not affect the emotional behavior of Cbg k.o. females. As Cbg k.o. males, Cbg k.o. females have markedly reduced corticosterone levels across the circadian cycle and also after stress. Plasma free corticosterone levels in Cbg k.o. mice measured immediately after stress were blunted in both sexes compared to WT mice. A trend for higher mean levels of ACTH in Cbg k.o. mice was found for both sexes. The turnover of a corticosterone bolus was increased in Cbg k.o. Finally, the glucocorticoid-regulated immediate early gene early growth response 1 (Egr1) showed a blunted mRNA expression in the hippocampus of Cbg k.o. mutants while mineralocorticoid and glucocorticoid receptors presented sex differences but equivalent mRNA expression between genotypes. Thus, in our experimental conditions, sex differences for despair-like behavior in WT mice are explained by estrogens levels. Also, in both sexes, the presence of CBG is required to attain optimal glucocorticoid concentrations and normal emotional reactivity, although in females this is apparent only under low E2 concentrations. These findings suggest a complex interaction of CBG and E2 on emotional reactivity in females. PMID:25244639

Minni, A M; de Medeiros, G F; Helbling, J C; Duittoz, A; Marissal-Arvy, N; Foury, A; De Smedt-Peyrusse, V; Pallet, V; Moisan, M P

2014-12-01

262

Diagnostic Significance of the Serum Thyroxine Binding Globulin (TBG) in Various Thyroid Diseases  

Energy Technology Data Exchange (ETDEWEB)

In an attempt to evaluate the diagnostic significance of the serum thyroxine binding globulin (TBG) in various thyroid disease states, the authors measured serum T{sub 3} uptake, T{sub 3}, total T{sub 4}, free T{sub 4}, TSH and TBG by radioimmunoassay technique, and calculated the free T{sub 4} index(FT{sub 4}I) and T{sub 4}/TBG ratio in 10 cases of normal subjects, 11 eases of hypothyroidism, 62 cases of euthyroidism and 37 cases of hyperthyroidism. The data were analysed in the aspects of diagnostic significance in each thyroid disease state, and the results were as follows; 1) In 10 cases of normal subjects, serum TBG was 17.4-26.8 ug/ml, FT{sub 4}I was 5.1-9.7, and T{sub 4}/TBG ratio was 21.9{sub 4}9.9 (Mean+-S.D.). 2) 62 cases of euthyroidism with diffuse and nodular goiter, FT{sub 4}I was 7.26+-1.82, T{sub 4}/TBG ratio was 31.47+-10.42, and there were no significant difference from those of normal subjects(p>0.5). 3) In 11 cases of hypothyroidism, the FT{sub 4}I was 3.13+-2.15, T{sub 4}/TBG ratio was 11.3+-5.31, significantly lower than normal controls(p<0.01). 4) In 37 cases of hyperthyroidism, the FT{sub 4}I was 30.0+-12.01 T{sub 4}/TBG ratio was 121.4+-62.2, significantly higher than normal controls(p<0.01). 5) There were significant correlations between the FT{sub 4}I and T{sub 4}/TBG ratio, total T{sub 4} and T{sub 4}/TBG radio, in each thyroid function states. 6) The FT{sub 4}I showed 100% of diagnostic value in hyperthyroidism, 89.2% in euthyroidism, and 80% in hypothyroidism group. The T{sub 4}/TBG ratio showed 100% of diagnostic value in hyperthyroidism and hypothyroidism, and 80.6% in euthyroidism group. The above results suggest that T{sub 4}/TBG ratio and FT{sub 4}I showed same diagnostic value in hyperthyroidism group, but T{sub 4}/TBG showed higher diagnostic significance than FT{sub 4}I in hypothyroidism.

Han, Bong Heon; Lee, Houn Young; Ko, Suk Man; Yoon, Sang Ryong; Ro, Heung Kyu [Chungnam National University College of Medicine, Daejeon (Korea, Republic of)

1981-09-15

263

Estimation of the T{sub 4} Binding Capacity of Serum Thyroxine Binding Globulin  

Energy Technology Data Exchange (ETDEWEB)

The most commonly used methods for determining thyroxine binding globulin (TBG) concentration as the total thyroxine-binding capacity utilize electrophoretic separation of serum. Although technically simple, the electrophoretic method is time consuming and is limited in the number of samples which can be run in a single assay. The author presented a single T{sub 4} load ion exchange resin method as an approach to simplify the technique as with clinical practicability and results were analyzed. For construction of the standard curves, serum mixtures were diluted with barbital buffer which effectively blocked T{sub 4}-binding to TBPA. For each serum dilution, a constant amount of T{sub 4}-{sup 125}I and increments of unlabelled T{sub 4} were added. After incubation in water bath, resin beads were dispensed to the samples which binded all T{sub 4} not bound to TBG. The radioactivity in the supernatant was counted in the gamma scintillation counter. Each standard curve was plotted from the percent counts in the supernatant and total T{sub 4} in each tube. Unknown samples were diluted to 1 : 40 and ran at a single T{sub 4} loading concentration, and the TBG capacity of the samples was able to be read on the standard isobars. The following results were obtained. 1) Mean and standard deviation for TBG capacity in normal population was 28.6+-5.09 mug T{sub 4}/100 ml. 2) 24.9+-3.87 mug T{sub 4}/100 ml in hyperthyroidism showed low TBG capacity tendency comparing to normal population(p<0.025). 3) 31.0+-2.40 mug T{sub 4}/100 ml in hypothyroidism showed high TBG capacity tendency comparing to normal population. 4) Reversed correlationship existed between TBG capacity and T{sub 3} resin uptake (r=-0.624), TBG capacity and serum T{sub 4} value (r=-0.859), and TBG capacity and free thyroxine index (r=-0.623). The author assumes that this method of assay is considerably simpler in instrumentation and technique than any other assays traditionally being used, and seems to be more practical for routine clinical laboratory use.

Lee, Kyung Ja; Koh, Chang Soon; Lee, Mun Ho [Seoul National University College of Medicine, Seoul (Korea, Republic of)

1973-09-15

264

Cardiovascular risk factors in men : The role of gonadal steroids and sex hormone-binding globulin  

DEFF Research Database (Denmark)

Males have higher risk of cardiovascular disease (CVD) than premenopausal females. Gonadal steroids are probably involved in the gender difference in CVD, but previous results have been conflicting. We investigated the associations between CVD risk factors and sex hormones in a cross-sectional designed study of 508 healthy males, aged 41 to 72 years. We determined total testosterone (T), sex hormone-binding globulin (SHBG), free androgen index (FAI), and estradiol (E2) and studied their relationship to body fat mass (BF), blood pressure (BP), aortic compliance, left ventricular mass (LVM), and plasma lipids (total cholesterol, high-density lipoprotein [HDL], low-density lipoprotein [LDL], very--low-density lipoprotein [VLDL], and triglycerides). In quartile analyses after adjustment for confounders (age, body mass index [BMI], alcohol consumption, and smoking), SHBG and E2 were positively associated with HDL, while FAI was negatively associated with HDL. T and SHBG were negatively associated with VLDL and triglycerides, while FAI was positively associated with VLDL and triglycerides. T and SHBG were negatively associated with BMI and BF, while FAI and E2 were positively associated with BMI and BF. E2 was negatively associated with LVM. No hormone varied with total cholesterol, LDL, BP, and aortic compliance in the adjusted analyses. In multiple regression analyses, SHBG was the main predictive variable of HDL, VLDL, and triglycerides explaining 12%, 17%, and 17% of the variation, respectively. No other hormones were selected as predictive variables for VLDL and triglycerides, but E2, T, and FAI were selected in the HDL regression, explaining 3%, 2%, and less than 1%, respectively. Our regression analyses illustrate the diverging results when investigating associations between gonadal steroids and lipids with and without SHBG adjustment. Atherogenic lipid profile in males is associated with low SHBG, low T levels, and a high FAI. Males with high E2 levels may have a less atherogenic lipid profile and lower LVM. SHBG is a key hormone in the association between sex hormones and plasma lipids. We suggest that conflicting results of cross-sectional and intervention studies of sex hormones and lipids, in part, may be explained by interindividual differences or changes in SHBG. Thus, further studies on the potential role of SHBG in the development of ischemic heart disease (IHD) should be performed.

Gyllenborg, J; Rasmussen, S L

2001-01-01

265

Molecular characterization of a genetic variant of the steroid hormone-binding globulin gene in heterozygous subjects  

Energy Technology Data Exchange (ETDEWEB)

Steroid hormone-binding globulin in human serum displays different isoelectric focusing (IEF) patterns among individuals, suggesting genetic variation in the gene for this extracellular steroid carrier protein. Analysis of allele frequencies and family studies suggested the existence of two codominant alleles of the gene. Subsequent determination of the molecular basis of a variant of the gene was carried out using DNA from homozygous individuals from a single Belgian family. It was of interest to characterize other variant individuals to determine whether all variants identified by IEF phenotyping were caused by the same mutation or whether other mutations occurred in the gene in different populations. Previous studies identified Mexican subjects who were heterozygous for the variant IEF phenotype. Denaturing gradient gel electrophoresis was used to localize the mutation in these subjects and to purify the variant allele for DNA sequence analysis. The results show that the mutation in this population is identical to that identified in the Belgian family, and no other mutations were detected in the gene. These data represent the first analysis of steroid hormone-binding globulin gene variation in heterozygous subjects and further support the conclusion of biallelism of the gene worldwide. 11 refs., 2 figs., 1 tab.

Hardy, D.O.; Catterall, J.F. [Population Council, New York, NY (United States); Carino, C. [Instituto National de la Nutricion, Mexico City, MX (United States)] [and others

1995-04-01

266

Genetic variability of albumin-globulin content, and lipoxygenase, peroxidase activities among bread and durum wheat genotypes  

Directory of Open Access Journals (Sweden)

Full Text Available The classical Osborne wheat proteins fraction (albumin-globulin, as well as several polypeptides from the non-gluten protein fraction using SDS-PAGE analyses were determined in the grain of five bread (T. aestivum L. and five durum wheat (T. durum Desf. genotypes. In addition, the activity rate of lipoxygenase (LOX and peroxidase (POD enzymes implicated in the antioxidant metabolism was determined. Albumins and globulins in wheat grains were characterized by rich protein pattern - the number of bands varied from 19 to 23 and they were defined by molecular weight 76.4-12.4 kDa. The great differences between bread and durum wheat polypeptide contents were found. Result pointed out that polypeptides with molecular weight of 65.6, 43.5 and 32.9 kDa could be used to differentiate the bread from durum wheat. Significant differences in the LOX and POD activity between and within two wheat species were detected. Present study showed in durum wheat a lower activities of LOX and POD enzymes for about 45 and 22%, respectively, than in bread wheat. Our results could be useful for plant breeders in screening and selecting of better raw materials with high protein quality for the flour, breadmaking and pasta industry.

Žili? Sla?ana

2011-01-01

267

Resolution of Mild Ganciclovir-Resistant Cytomegalovirus Disease with Reduced-Dose Cidofovir and CMV-Hyperimmune Globulin  

Science.gov (United States)

Ganciclovir-resistant cytomegalovirus (CMV) is associated with significant morbidity in solid organ transplant recipients. Management of ganciclovir-resistant CMV may be complicated by nephrotoxicity which is commonly observed with recommended therapies and/or rejection induced by “indirect” viral effects or reduction of immunosuppression. Herein, we report a series of four high serologic risk (donor CMV positive/recipient CMV negative) kidney transplant patients diagnosed with ganciclovir-resistant CMV disease. All patients initially developed “breakthrough” viremia while still receiving valganciclovir prophylaxis after transplant and were later confirmed to exhibit UL97 mutations after failing to eradicate virus on adequate dosages of valganciclovir. The patients were subsequently and successfully treated with reduced-dose (1-2?mg/kg) cidofovir and CMV-hyperimmune globulin, given in 2-week intervals. In addition, all patients exhibited stable renal function after completion of therapy, and none experienced acute rejection. The combination of reduced-dose cidofovir and CMV-hyperimmune globulin appeared to be a safe and effective regimen in patients with mild disease due to ganciclovir-resistant CMV. PMID:24991428

Patel, Samir J.; Kuten, Samantha A.; Knight, Richard J.; Hong, Dana M.; Gaber, A. Osama

2014-01-01

268

Physicochemical changes taking place in bovine globulins under the influence of gamma irradiation studied by thermal analysis  

International Nuclear Information System (INIS)

Radiation modification of protein based polymers as well as the development of gamma irradiation techniques as a method of food sterilisation and preservation induces necessity of better recognition of the physicochemical changes occurring in proteins after gamma irradiation. Recently differential scanning calorimetry (DSC) was applied widely in structural studies of proteins. Also thermoanalytical methods (TG, DTG) applied for proteins pyrolysis were also found to be useful in characterisation of proteins structure and the properties of proteins containing tissues. In presented paper, the aforementioned methods were applied for investigation of the gamma irradiation influence on thermal decomposition of gamma and alpha globulins and the results were related to their structural modifications. It has been found, that irradiation influences the course of decomposition of gamma and alpha globulins. Irradiation of solid native proteins result in decreased temperature of decomposition, especially decreased temperature of the last stage. The effects of irradiation performed for water suspensions were clearly more significant. The relatively large differences between decomposition of the irradiated and non-irradiated samples were detected by DSC and thermogravimetry already after irradiation of water suspensions

269

Hydrogen storage:  

OpenAIRE

The invention relates to the storage of hydrogen. The invention relates especially to storing hydrogen in a clathrate hydrate. The clathrate hydrate according to the present invention originates from a composition, which comprises water and hydrogen, as well as a promotor compound. The promotor compound provides a large reduction of the pressure needed and/or an increase of the temperature needed to form a clathrate hydrate. Also, the desorption of hydrogen gas from the clathrate hydrate is e...

Schlapbach, L.; Zu?ttel, A.; Pedersen, Allan Schrøder

2005-01-01

270

Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.  

Science.gov (United States)

11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress. PMID:20693668

Tandang-Silvas, Mary Rose; Carrazco-Peña, Laura; Barba de la Rosa, Ana Paulina; Osuna-Castro, Juan Alberto; Utsumi, Shigeru; Mikami, Bunzo; Maruyama, Nobuyuki

2010-08-01

271

Dossier: underground storage  

International Nuclear Information System (INIS)

This dossier reviews the main concepts of storage in geologic formations: shape of artificial cavities; natural reservoirs: natural gas storage in aquifers, heat storage, karsts and caves; artificial reservoirs: salt dissolution cavities, salt mines, enlargement of cavities, storage of metal wastes; reservoirs in mining cavities: hydrocarbons storage (tightness, steel coated cavities), cryogenic storage; use of ancient infrastructures (mines, quarries, galleries): hydrocarbons storage, toxic wastes storage, radioactive wastes disposal, reversible radioactive wastes storage, solar neutrons trapping in underground galleries, storage of film archives etc.. (J.S.)

272

Expression and Function of 3beta Hydroxisteroid Dehydrogenase (3? HSD) Type II and Corticosteroid Binding Globulin (CBG) in Granulosa Cells from Ovaries of Women with and without Endometriosis  

OpenAIRE

Purpose: To investigate the secretion of progesterone (P4) and corticosteroid binding globulin (CBG) by granulosa luteal cells (GC) as well as the mRNA levels of CBG and 3? hydroxisteroid dehydrogenase (3? HSD), in women with and without endometriosis in vivo and in vitro.

Garrido, Nicola?s; Kru?ssel, Jan S.; Remohi?, Jose?; Simo?n, Carlos; Pellicer, Antonio

2002-01-01

273

Fuel storage  

International Nuclear Information System (INIS)

ENSA is a well known manufacturer of multi-system primary components for the nuclear industry and is totally prepared to satisfy future market requirements in this industry. At the same time that ENSA has been gaining a reputation world wider for the supply of primary components, has been strengthening its commitment and experience in supplying spent fuel components, either pool racks or storage and transportation casks, and offers not only fabrication but also design capabilities for its products. ENSA has supplied Spent Fuel Pool Racks, in spain, Finland, Taiwan, Korea, China, and currently it is in the process of licensing its own rack design in the United States of America for the ESBWR along with Ge-Hitachi. ENSA has supplied racks for 20 pools and 22 different reactors and it has also manufactured racks under all available technologies and developed a design known as Interlock Cell Matrix whose main features are outlined in this article. Another ENSA achievement in rack technology is the use of remote control for re-racking activities instead of using divers, which improves the ALARA requirements. Regarding casks for storage and transportation, ENSA also has al leading worldwide position, with exports prevailing over the Spanish market where ENSA has supplied 16 storage and transportation casks to the Spanish nuclear power Trillo. In some cases, ENSA acts as subcontractor for other clients. Foreign markets are still a major challenge for ENSA. ENSA-is well knownjor challenge for ENSA. ENSA-is well known for its manufacturing capabilities in the nuclear industry, but has been always involved in design activities through its engineering division, which carries out different tasks: components Design; Tooling Design; Engineering and Documentation; Project Engineering; Calculations, Design and Development Engineering. (Author)

274

Storage pond  

International Nuclear Information System (INIS)

A pond is described for the storage of hazardous materials, such as irradiated nuclear fuel elements, under water. Upper and lower impervious membranes extend without interruption beneath the floor of the pond and the edges of the membranes lead into a trench surrounding the pond. Any leakage through the floor is directed normally by the upper membrane into the trench. The lower membrane provides an additional impervious barrier in the event of a leak in the upper membrane and again directs the leakage into the trench thereby avoiding contamination of the ground beneath the pond. (author)

275

Gas storage materials, including hydrogen storage materials  

Energy Technology Data Exchange (ETDEWEB)

A material for the storage and release of gases comprises a plurality of hollow elements, each hollow element comprising a porous wall enclosing an interior cavity, the interior cavity including structures of a solid-state storage material. In particular examples, the storage material is a hydrogen storage material, such as a solid state hydride. An improved method for forming such materials includes the solution diffusion of a storage material solution through a porous wall of a hollow element into an interior cavity.

Mohtadi, Rana F; Wicks, George G; Heung, Leung K; Nakamura, Kenji

2014-11-25

276

Isoforms of thyroxine-binding globulin as a model for molecular epidemiology of human cancer risk  

International Nuclear Information System (INIS)

The novel field of molecular epidemiology of human cancer risk has added a new branch to classical epidemiology by providing a direct link between human cancer and carcinogen exposure. It was estimated that about 80% of cancers are due to environmental factors. The blood proteins are almost certainly targets for modification in human cancer, and their identification and characterization will be of primary importance in the development of the new and rapidly evolving field of molecular epidemiology. Among blood proteins that are altered in human cancer, TBG occupies a special place because the level of human blood TBG is the most sensitive to intensification of biosynthesis and proliferation processes in organisms in different types of cancer. The increase of TBG concentration in cancer can be result from both activation of TBG biosynthesis in liver or altering of post translation glycosylation that prolongs protein survival time. The molecular basis for the change in the properties of TBG in cancer is unknown. These distinctive changes could have important consequences for the function of TBG in cancer and may help to develop more precise markers for monitoring pathological progression in this disease. Considerable variability and subtlety can occur in the carbohydrate composition and structure of serum glycoproteins in disease. This can be either as a major change, such as an increase in the number of oligosaccharide branches at a particular glycosylation site or as a minor change such as the addition of an extra fucose or sialic acid residue. Increased fucosylation has also been reported for transferrin and alpha-fetoprotein in liver cancer; thyroglobulin in thyroid cancer, IgG in myeloma, haptoglobin in ovarian cancer. The last own studies have shown that in clinically healthy teenagers born in Khojniki (137 Cs 185-555 kBq/m), we have found an unusual thyroid profile exhibiting increased levels of total triiodothyronine (T3), total thyroxine (T4), and thyroxine-binding globulin (TBG) by 50-60%, but no elevation in free T4, as compared with similar control groups of from Minsk. One of the reasons for the euthyroid syndrome in teenagers from the contaminated region was shown to be the blood isoforms in TBG. These isoforms differed in the structure of the carbohydrate components and as a rule are characterized by a prolonged circulation time. Using specific affinity chromatography, with subsequent immunological assay we shown that a certain amount of serum TBG-molecules in persons from Khojniki contains a high level of TBG (about 30% higher than the control group) containing fucosyalated biantennary sugar chains with more prolonged survival time. Previous findings showed that increased levels of fucosylation are observed for some serum glycoproteins in the blood of tumour patients. We provide evidence for variations in the TBGfuc which are contained in the serum TBG pool of patients suffering from cancer with various localisation. Thus, analysis of TBGfuc may be regarded as a prognostic marker in the determination of risk groups. The molecular mechanism of increased activity of fucosyl transferases will be discussed. We propose to measure the level of TBG fucosylated to assess the human cancer risk and habitants of contaminated region of Belarus. (authors)

277

Linkage mapping of '25-kDa globulin' genes on homoeologous group-1 chromosomes of bread and durum wheat.  

Science.gov (United States)

Acid polyacrylamide-gel electrophoresis (A-PAGE) of ethanol-soluble proteins from the endosperm of bread and durum wheats reveals some bands encoded by genes on the homoeologous group-1 chromosomes with higher mobility than the ?-gliadins. The isolation of these proteins showed that they were the previously described '25-kDa globulins' encoded by genes at the Glo-A1, Glo-B1, and Glo-D1 loci. The variability found among a collection of 51 bread and 81 durum wheats was very low: two allelic variants at Glo-A1 and no variants at Glo-B1 in each of the two species, and two allelic variants at Glo-D1 in bread wheat. Inheritance studies of '25-kDa globulin' genes on group-1 chromosomes of bread and durum wheat were carried out on the F2 progeny from four crosses, two in bread wheat and two in durum wheat. The linkage mapping of the 1A '25-kDa globulin' genes of bread wheat was done based on four prolamin loci: Glu-A1, Glu-A3, Gli-A1 and Gli -A3. The percentages of recombination and the distances found allowed a re-evaluation of the linkage map of endosperm protein loci on this chromosome. The Glo-A1 locus was found to be located at the distal end of the short arm of 1A chromosome, at a distance of 5.23±1.99 cM from Gli-A1, 6.85±2.22 cM from Glu-A3, 22.64±3.62 cM from Gli-A1, and at a recombination percentage of 49.30±4.40 from Glu-A1. A similar distance between Gli-A1 and Glo-A1 (4.82±1.75 and 6.66±2.26 cM) was found in durum wheat. The distance between Gli-D1 and Glo-D1 on chromosome 1D was 2.86±1.39 cM. PMID:24162408

Nieto-Taladriz, M T; Pernas, M; Salcedo, G; Carrillo, J M

1996-10-01

278

LENTIL TANNIN-GLOBULIN INTERACTIONAND AND IN VITRO HYDROLYSIS INTERAÇÃO TANINO-GLOBULINA DE LENTILHA E HIDRÓLISE IN VITRO  

Directory of Open Access Journals (Sweden)

Full Text Available Protein fractions were isolated from lentil cotyledons and tannins were isolated and purified from lentil seed coats. The globulin fraction corresponded to 42.7% of the total lentil flour nitrogen, representing the major protein fraction. Acetone:water (7:3 was the best extractant for seed coat tannins compared to methanol or methanol-HCl 1%. Native and heated (99oC/15 min. isolated lentil globulin and casein were hydrolyzed with trypsin and pepsin in the absence of tannins and at 1:40, 1:20, 1:10, 1:5 and 1:2.5 tannin-to-protein ratios. The tryptic and peptic hydrolysis of the unheated proteins were reduced with increasing tannin-to-protein ratios. Unheated casein showed to be more susceptible to trypsin than globulin and the opposite effect was observed with pepsin. Heating followed by tannin interaction and hydrolysis had a more pronounced effect on tryptic than peptic digestion for both proteins.As frações protéicas foram isoladas dos cotiledones e os taninos isolados e purificados da casca da lentilha. A fração globulina correspondeu a 42,7 % do nitrogenio total da farinha de lentilha representando a fração protéica majoritária. Comparativamente ao metanol e metanol-HCl 1% a mistura acetona:água (7:3 representou o melhor meio extrator para os taninos da casca. A fração globulina isolada, nativa e aquecida (99oC/15 min, e caseína foram hidrolisadas com tripsina e pepsina na ausência de taninos e na presença de relações tanino:proteína de 1:40, 1:20, 1:10, 1:5 e 1:2,5. A hidrólise tríptica e péptica das proteínas não-aquecidas foram reduzidas com o aumento da relação tanino-proteína. A caseína não aquecida mostrou ser mais susceptível à tripsina que à globulina, o oposto sendo observado com a pepsina. O aquecimento seguido de interação com os taninos e hidrólise teve um efeito mais pronunciado sobre a digestão com tripsina que com pepsina para ambas proteínas.

Valdir A. NEVES

1998-08-01

279

Comparative study regarding the association of alpha-2U globulin with the nephrotoxic mechanism of certain petroleum-based Air Force fuels. Final report, 1 September 1986-31 August 1987  

Energy Technology Data Exchange (ETDEWEB)

Alpha-2U globulin is a low-molecular-weight urinary protein which may be associated with a hydrocarbon-induced proximal tubular cell degeneration in the male rat kidney. A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion exchange and hydroxylapatite chromatography. Isoelectric focusing techniques were developed to isolate the major isoelectric variants of the alpha-2U globulin molecule and to assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds. Alpha-2U globulin was isolated from the urine of albino and pigmented male rats to study strain susceptibility to the nephrotoxic process. An alpha-2u globulin isoelectric variant profile distinguishing albino from non-albino male rats was not apparent, however, strain differences were revealed. Fischer 344 male rats appear to have higher levels of the isoelectric variants than the other strains studied. These findings suggest that if a strain susceptibility to the hydrocarbon-induced nephrotoxic lesion exists, it may be associated with the alpha-2U globulin isoelectric variant profile.

Eurell, T.E.

1987-10-27

280

d-Limonene-induced male rat-specific nephrotoxicity: Evaluation of the association between d-limonene and alpha 2u-globulin  

International Nuclear Information System (INIS)

d-Limonene is a naturally occurring monoterpene, which when dosed orally, causes a male rat-specific nephrotoxicity manifested acutely as the exacerbation of protein droplets in proximal tubule cells. Experiments were conducted to examine the retention of [14C]d-limonene in male and female rat kidney, to determine whether d-limonene or one or more of its metabolites associates with the male rat-specific protein, alpha 2u-globulin, and if so, to identify the bound material. The results indicated that, 24 hr after oral administration of 3 mmol d-limonene/kg, the renal concentration of d-limonene equivalents was approximately 2.5 times higher in male rats than in female rats. Equilibrium dialysis in the presence or absence of sodium dodecyl sulfate indicated that approximately 40% of the d-limonene equivalents in male rat kidney associated with proteins in a reversible manner, whereas no significant association was observed between d-limonene equivalents and female rat kidney proteins. Association between d-limonene and male rat kidney proteins was characterized by high-performance gel filtration and reverse-phase chromatography. Gel filtration HPLC indicated that d-limonene in male rat kidney is associated with a protein fraction having a molecular weight of approximately 20,000. Separation of alpha 2u-globulin from other kidney proteins by reverse-phase HPLC indicated that d-limonene associated with a protein present only in male rat kidney which was definit only in male rat kidney which was definitively identified as alpha 2u-globulin by amino acid sequencing. The major metabolite associated with alpha 2u-globulin was d-limonene-1,2-oxide. Parent d-limonene was also identified as a minor component in the alpha 2u-globulin fraction

281

Effect of phosphorus on the composition and accumulation of 7S and 11S globulin subunits during seed development of three soybean varieties  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in english Three soybean varieties (Dongnong 42, high-protein cultivar; Hefeng 25, medium-protein; and Dongnong 46, low-protein cultivar) grown under different P conditions were investigated in order to evaluate the effect of P on the composition and accumulation of 7S and 11S globulin subunits. The soybean se [...] eds were planted in pots and fertilized with 0.033 g of N and K2O per kg soil. Four levels of P treatment were designed, which were P1, P2, P3 and P4 (i.e., 0, 0.033, 0.067, and 0.100 g of phosphorus pentoxide (P2O5) per kilogram of soil). The composition of extractable proteins was determined by conducting SDS-PAGE. No significant differences were observed between the varieties with regard to the molecular weights of the globulin subunits. After the subunits were formed, their concentrations gradually increased under different P treatments, reached a peak at 70 d post-anthesis (DPA), and then declined during maturity. The concentrations of the 7S and 11S globulins and other subunits in Dongnong 42 and Hefeng 25 were the highest under the P3 treatment, while they were the highest in Dongnong 46 under the P2 treatment. Under the same P treatment, the concentrations were high in Dongnong 42 and low in Dongnong 46 and Hefeng 25. Significant differences were observed in the concentrations of 7S and 11S globulin subunits between the varieties and levels of P treatments, especially under the P3 treatment. Alpha subunit was mainly in relation with the P-nutrition in the three varieties. These findings suggested that the; optimal combination of cultivarsand P treatment; levels could contribute to the increased concentrations of both soybean globulins and their subunits.

J, Han; W.G, Jie; B.Y, Cai.

2014-12-01

282

A phase II multicenter rabbit anti-thymocyte globulin trial in patients with myelodysplastic syndromes identifying a novel model for response prediction.  

Science.gov (United States)

Immune dysregulation is a mechanism contributing to ineffective hematopoiesis in a subset of myelodysplastic syndrome patients. We report the first US multicenter non-randomized, phase II trial examining the efficacy of rabbit(r)-anti-thymocyte globulin using 2.5 mg/kg/day administered daily for 4 doses. The primary end point was hematologic response; secondary end points included duration of response, time to response, time to progression, and tolerance. Nine (33%;95% confidence interval=17%-54%) of the 27 patients treated experienced durable hematologic improvement in an intent-to-treat analysis with a median time to response and median response duration of 75 and 245 days, respectively. While younger age is the most significant factor favoring equine(e)-anti-thymocyte globulin response, treatment outcome on this study was independent of age (P=0.499). A shorter duration between diagnosis and treatment showed a positive trend (P=0.18), but International Prognostic Scoring System score (P=0.150), karyotype (P=0.319), and age-adjusted bone marrow cellularity (P=0.369) were not associated with response classification. Since activated T-lymphocytes are the primary cellular target of anti-thymocyte globulin, a T-cell expression profiling was conducted in a cohort of 38 patients consisting of rabbit and equine-antithymocyte globulin-treated patients. A model containing disease duration, CD8 terminal memory T cells and T-cell proliferation-associated-antigen expression predicted response with the greatest accuracy using a leave-one-out cross validation approach. This profile categorized patients independent of other covariates, including treatment type and age using a leave-one-out-cross-validation approach (75.7%). Therefore, rabbit-anti-thymocyte globulin has hematologic remitting activity in myelodysplastic syndrome and a T-cell activation profile has potential utility classifying those who are more likely to respond (NCT00466843 clinicaltrials.gov). PMID:24488560

Komrokji, Rami S; Mailloux, Adam W; Chen, Dung-Tsa; Sekeres, Mikkael A; Paquette, Ronald; Fulp, William J; Sugimori, Chiharu; Paleveda-Pena, Jennifer; Maciejewski, Jaroslaw P; List, Alan F; Epling-Burnette, Pearlie K

2014-07-01

283

Effect of phosphorus on the composition and accumulation of 7S and 11S globulin subunits during seed development of three soybean varieties  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in english Three soybean varieties (Dongnong 42, high-protein cultivar; Hefeng 25, medium-protein; and Dongnong 46, low-protein cultivar) grown under different P conditions were investigated in order to evaluate the effect of P on the composition and accumulation of 7S and 11S globulin subunits. The soybean se [...] eds were planted in pots and fertilized with 0.033 g of N and K2O per kg soil. Four levels of P treatment were designed, which were P1, P2, P3 and P4 (i.e., 0, 0.033, 0.067, and 0.100 g of phosphorus pentoxide (P2O5) per kilogram of soil). The composition of extractable proteins was determined by conducting SDS-PAGE. No significant differences were observed between the varieties with regard to the molecular weights of the globulin subunits. After the subunits were formed, their concentrations gradually increased under different P treatments, reached a peak at 70 d post-anthesis (DPA), and then declined during maturity. The concentrations of the 7S and 11S globulins and other subunits in Dongnong 42 and Hefeng 25 were the highest under the P3 treatment, while they were the highest in Dongnong 46 under the P2 treatment. Under the same P treatment, the concentrations were high in Dongnong 42 and low in Dongnong 46 and Hefeng 25. Significant differences were observed in the concentrations of 7S and 11S globulin subunits between the varieties and levels of P treatments, especially under the P3 treatment. Alpha subunit was mainly in relation with the P-nutrition in the three varieties. These findings suggested that the; optimal combination of cultivarsand P treatment; levels could contribute to the increased concentrations of both soybean globulins and their subunits.

J, Han; W.G, Jie; B.Y, Cai.

284

Presence of the storage seed protein vicilin in internal organs of larval Callosobruchus maculatus (Coleoptera: Bruchidae).  

Science.gov (United States)

Variant vicilins (7S storage globulins) of cowpea seeds (Vigna unguiculata) are considered as the main resistance factor present in some African genotypes against the bruchid Callosobruchus maculatus. It has been suggested that the toxic properties of vicilins may be related to their recognition and interaction with glycoproteins and other membrane constituents along the digestive tract of the insect. However, the possibility of a systemic effect has not yet been investigated. The objective of this work was to study the fate of 7S storage globulins of V. unguiculata in several organs of larvae of the cowpea weevil C. maculatus. Results demonstrated binding of vicilins to brush border membrane vesicles, suggesting the existence of specific receptors. Vicilins were detected in the haemolymph, in the midgut, and in internal organs, such as fat body and malpighian tubules. There is evidence of accumulation of vicilins in the fat body of both larvae and adults. The absorption of vicilins and their presence in insect tissues parallels classical sequestration of secondary compounds. PMID:16288905

Uchôa, Adriana F; DaMatta, Renato A; Retamal, Claudio A; Albuquerque-Cunha, José M; Souza, Sheila M; Samuels, Richard I; Silva, Carlos P; Xavier-Filho, José

2006-02-01

285

Redox changes accompanying storage protein mobilization in moist chilled and warm incubated walnut kernels prior to germination.  

Science.gov (United States)

Alterations in the redox state of storage proteins and the associated proteolytic processes were investigated in moist-chilled and warm-incubated walnut (Juglans regia L.) kernels prior to germination. The kernel total protein labeling with a thiol-specific fluorochrome i.e. monobromobimane (mBBr) revealed more reduction of 29-32 kDa putative glutelins, while in the soluble proteins, both putative glutelins and 41, 55 and 58 kDa globulins contained reduced disulfide bonds during mobilization. Thus, the in vivo more reduced disulfide bonds of storage proteins corresponds to greater solubility. After the in vitro reduction of walnut kernel proteins pre-treated by N-ethyl maleimide (NEM) with dithioerythrethiol (DTT) and bacterial thioredoxin, the 58 kDa putative globulin and a 6 kDa putative albumin were identified as disulfide proteins. Thioredoxin stimulated the reduction of the H(2)O(2)-oxidized 6 kDa polypeptide, but not the 58 kDa polypeptide by DTT. The solubility of 6 kDa putative albumin, 58 and 19-24 kDa putative globulins and glutelins, respectively, were increased by DTT. The in vitro specific mobilization of the 58 kDa polypeptide that occurred at pH 5.0 by the kernel endogenous protease was sensitive to the serine-protease inhibitor phenylmethylsulfonyl fluoride (PMSF) and stimulated by DTT. The specific degradation of the 58 kDa polypeptide might be achieved through thioredoxin-mediated activation of a serine protease and/or reductive unfolding of its 58 kDa polypeptide substrate. As redox changes in storage proteins occurred equally in both moist chilled and warm incubated walnut kernels, the regulatory functions of thioredoxins in promoting seed germination may be due to other germination related processes. PMID:22989946

Shahmoradi, Zeynab; Tamaskani, Fatemeh; Sadeghipour, Hamid Reza; Abdolzadeh, Ahmad

2013-01-01

286

[The macrophage disappearance reaction in guinea pigs sensitized with bovine gamma globulin or human scrum albumin (author's transl)].  

Science.gov (United States)

The macrophage disappearance reaction (MDR) is a suitable test for detection of cell mediated immunity against bovine gamma globulin (BGG) and human serum albumin (HSA) in guinea pigs. The MDR is a technical simple, good manipulable, and quantifiable test. The optimal test conditions for the antigens BGC and HSA are the following: Peritoneal exudat cells (PEC) were stimulated with paraffin oil. On the 5th day after receiving oil the animals were injected with 80 microgram BGG or 30 microgram HSA i.p. 5 hours later the PEC were harvested and counted. With the MDR it is possible to detect differences with respect to degree of cell-mediated immunity. Supernatants of sensitized lymphocytes produces the MDR too. PMID:69396

Schimke, R; Bernstein, B; Ambrosius, H

1977-01-01

287

[Hematologic improvement with deferasirox following tandem antithymocyte globulin treatment in a transfusion-dependent patient with severe aplastic anemia].  

Science.gov (United States)

A 62-year-old man with transfusion-dependent severe aplastic anemia received immunosuppressive therapy (IST) with rabbit antithymocyte globulin and cyclosporine A in April 2010. However, his transfusion dependency did not improve. As more than 100 red blood cell (RBC) transfusions had been performed, he was administered iron chelation therapy (ICT) with deferasirox (DFX) to improve iron overload starting in July 2011. Consequently, both RBC and platelet transfusion dependency gradually improved concomitant with a decrease in serum ferritin. The bone marrow (BM) biopsy findings before administration of DFX showed severe iron accumulation and strong positive immunostaining for 8-OHdG, a marker of oxidative stress due to free iron. One year after ICT, the number of BM hematopoietic cells was increased and both iron deposition and oxidative stress were decreased. These findings suggest that DFX may contribute to hematological improvement in patients with IST-refractory aplastic anemia. PMID:24305537

Jomen, Wataru; Kuroda, Hiroyuki; Yamada, Michiko; Matsuno, Teppei; Sato, Masanori; Abe, Tomoyuki; Sakurai, Tamaki; Fujii, Shigeyuki; Maeda, Masahiro; Fujita, Miri; Nagashima, Kazuo; Iyama, Satoshi; Miyanishi, Koji; Kobune, Masayoshi; Kato, Junji

2013-11-01

288

Radioimmunoassay for serum thyroxine-binding globulin: results in normal subjects and in patients with hepatocellular carcinoma. [/sup 125/I  

Energy Technology Data Exchange (ETDEWEB)

Serum thyroxine-binding globulin (TBG) was measured by radioimmunoassay. The human TBG used in this study was purified by affinity, anion-exchange, and gel filtration chromatography. The serum TBG concentration in 98 euthyroid normals was 1.48 +- 0.46 mg/100 ml (mean +- SD), which is one-half that previously reported using a similar method. The level in females (1.66 +- 0.56) was significantly higher than that in males (1.37 +- 0.37). Comparison of the serum TBG level and the maximum binding capacity of serum TBG for thyroxine (T/sub 4/) yields a molar ratio of 1:1 for T/sub 4/ and TBG. The mean serum TBG in 19 patients with hepatocellular carcinoma was 2.10 +- 1.29 mg/100 ml; however, only two of these patients had serum TBG levels outside the normal range.

Gershengorn, M.C.; Larsen, P.R.; Robbins, J.

1976-05-01

289

Binding of Ochratoxin A to a Urinary Globulin: A New Concept to Account for Gender Difference in Rat Nephrocarcinogenic Responses  

Directory of Open Access Journals (Sweden)

Full Text Available SDS-gradient mini-gel electrophoresis and immunoblotting of urine of rats given ochratoxin A (OTA, showed OTA binding to an α2u-globulin. Perceived potential internalised delivery of OTA to proximal tubule epithelia by the carrier, specific only to adult male rats and augmenting other uptake mechanisms, suggests that some experimental nephrotoxicological data may not be appropriate for human risk assessment. Reexamination of female rat renal tumour histopathology of the NTP high dose OTA study showed all carcinomas were solitary, unilateral, microscopic and clinically insignificant at the 2-year end-stage. The novel concept, when consolidated further from our archived material, may moderate current perceptions of the human risk of traces of dietary OTA.

Judit Nagy

2008-05-01

290

Intractable Seizure in a Case with Parry-Romberg Syndrome and Good Response to Intravenous Immune Globulin  

Directory of Open Access Journals (Sweden)

Full Text Available Objective: Parry-Romberg syndrome is a rare disorder associated with unilateral facial atrophy involving skin, subcutaneous tissue, skeletal muscles and bone. Occasionally, there is central nervous system involvement with epilepsy being the most common manifestation. Case report: In this article, we report a 12 year-old boy with Parry-Romberg syndrome. He had seizures since he was 5 years old, since 1 year ago the seizure attacks were refractory in spite of taking several antiepileptic drugs. Significant improvement was obtained after administration of intravenous immune-globulin (IVIG. Conclusion: IVIG may be of benefit when seizures in Parry-Romberg syndrome are intractable and resistant to antiepileptic drugs.

F Ashrafzadeh

2006-05-01

291

Effect of chickpea (Cicer arietinum L.) germination on the major globulin content and in vitro digestibility Efeito da germinação de grão-de-bico (Cicer arietinum L.) na globulina majoritária e digestibilidade in vitro  

OpenAIRE

Chickpea seed germination was carried out over a period of 6 days. Little variation in the nitrogen and total globulin content was observed. The major globulin (11 S type) showed higher variation after the 4th day of germination. The elution behaviour and distribution of the isolated major globulin fraction on Sepharose CL-6B chromatography showed little modification at the end of germination. On SDS-PAGE the peak eluted from Sepharose CL-6B showed changes in protein bands between 20 and 30 k...

Guilherme Vanucchi Portari; Olga Luisa Tavano; Da Silva, Maraiza A.; Valdir Augusto Neves

2005-01-01

292

Serum testosterone, sex hormone-binding globulin and total calcium levels predict the calcaneal speed of sound in men  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english OBJECTIVES: Variations in sex hormones and the calcium balance can influence bone health in men. The present study aimed to examine the relationship between the calcaneal speed of sound and biochemical determinants of bone mass, such as sex hormones, parathyroid hormones and serum calcium. METHODS: [...] Data from 549 subjects from the Malaysian Aging Male Study, which included Malay and Chinese men aged 20 years and older residing in the Klang Valley, were used for analysis. The subjects' calcaneal speed of sound was measured, and their blood was collected for biochemical analysis. Two sets of multiple regression models were generated for the total/bioavailable testosterone and estradiol to avoid multicollinearity. RESULTS: The multiple regression results revealed that bioavailable testosterone and serum total calcium were significant predictors of the calcaneal speed of sound in the adjusted model. After adjustment for ethnicity and body mass index, only bioavailable testosterone remained significant; the total serum calcium was marginally insignificant. In a separate model, the total testosterone and sex hormone-binding globulin were significant predictors, whereas the total serum calcium was marginally insignificant. After adjustment for ethnicity and body mass index (BMI), the significance persisted for total testosterone and SHBG. After further adjustment for age, none of the serum biochemical determinants was a significant predictor of the calcaneal speed of sound. CONCLUSION: There is a significant age-dependent relationship between the calcaneal speed of sound and total testosterone, bioavailable testosterone and sex hormone-binding globulin in Chinese and Malay men in Malaysia. The relationship between total serum calcium and calcaneal speed of sound is ethnicity-dependent.

Kok-Yong, Chin; Ima-Nirwana, Soelaiman; Isa Naina, Mohamed; Wan Zurinah Wan, Ngah.

2012-08-01

293

In vivo release of testosterone from. gamma. -globulin-drug delivery composites made by radiation after melt-pressing  

Energy Technology Data Exchange (ETDEWEB)

The rigid ..gamma..-globulin matrix (50 mg) as a carrier for drug delivery system was made by ..gamma..-ray irradiation after melt-pressing at 75/sup 0/C under a pressure of 100 kg/cm/sup 2/. The in vivo degradation (weight loss) of the matrix when implanted subcutaneously in the back of wistar rats was about 1.9% at 90th day from implantation. When 0.1 M Tartarate buffer solution (pH 1.8) containing 0, 0.005, 0.01 and 0.1 w/v% pepsin was used as a digestive medium (37/sup 0/C), the in vitro degradation (weight loss) of the matrix was 1.2, 31.4, 45.7 and 53.9% at 90th day from start of the test, respectively. Therefore, it was concluded that the in vivo degradation of the matrix was much slower than that in vitro. On the basis of these results, testosterone (15 mg) as a drug was entrapped in ..gamma..-globulin matrix irradiated after melt-pressing. The in vivo degradation of matrix itself was significantly accelerated in the presence of drug, and reached up to 57.4% at 90th day from implantation. The in vivo release of drug from the composites was investigated using castrated Wistar rats. Furthermore, the study of the relationship between the in vivo release of drug and the physiological response (change in weight of the ventral prostate) indicated that the efficacious drug release continued up to 60 days.

Asano, Masaharu; Yoshida, Masaru; Kaetsu, Isao (Japan Atomic Energy Research Inst., Takasaki, Gunma. Takasaki Radiation Chemistry Research Establishment); Nakai, Katsuyuki; Yamanaka, Hidetoshi; Yuasa, Hisako; Shida, Keizo

1983-09-01

294

Spatial Information Storage  

Directory of Open Access Journals (Sweden)

Full Text Available The article describes the spatial information storage, shows the features of spatial information and of such storage systems formation. Requirements for information storage technologies and for the data management in storage systems are determined. Cartographic information storage and updating features are shown. The article proves that intelligent solutions are the most efficient means of working with large amounts of spatial data.

Vladimir Markelov

2013-01-01

295

Antihypertensive Peptides from Vicilin, the Major Storage Protein of Mung Bean (Vigna radiata (L.) R. Wilczek)  

OpenAIRE

Hypertension is among the leading diseases afflicting humans and the search for cheap and alternative modes of treatment is of primary importance. This study investigated the potential of vicilin, the major storage protein of mung bean, to generate antihypertensive peptides. The total soluble proteins of mung bean var. Pag-asa 7 were extracted using 35 mM potassium phosphate buffer (pH 7.0) containing 0.40 M NaCl. Vicilin (8S globulin) was purified by a combination of ammonium sulfate f...

Angelia, M. R. N.; Torio, M. A. O.; Garcia, R. N.; Viernes, L. B. G.

2012-01-01

296

The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT, enzyme-linked immunosorbent assay (ELISA, immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes. Conclusions The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2.

Sirvent Sofía

2012-12-01

297

The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut  

Science.gov (United States)

Background The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut. Methods Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT), enzyme-linked immunosorbent assay (ELISA), immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed. Results The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut) but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes. Conclusions The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2. PMID:23231956

2012-01-01

298

Storage Pool Deficiencies  

Science.gov (United States)

... Issues for Girls & Women Treatment Options Resources Inherited Platelet Disorders Bernard-Soulier Syndrome Glanzmann Thrombasthenia Storage Pool Deficiencies Home About Bleeding Disorders Storage pool ...

299

Energy storage criteria handbook  

Science.gov (United States)

The purpose of this handbook is to provide information and criteria necessary for the selection and sizing of energy storage technologies for use at U.S. Naval facilities. The handbook gives Naval base personnel procedures and information to select the most viable energy storage options to provide the space conditioning (heating and cooling) and domestic hot water needs of their facility. The handbook may also be used by contractors, installers, designers, engineers, architects, and manufacturers who intend to enter the energy storage business. The handbook is organized into three major sections: a general section, a technical section, and an example section. While a technical background is assumed for the latter two sections, the general section is simply written and can serve as an introduction to the field of energy storage. The technical section examines the following energy storage technologies: sensible heat storage, latent heat storage, cold storage, thermochemical storage, mechanical storage, pumped hydro storage, and electrochemical storage. The example section is limited to thermal storage and includes examples for: water tank storage, rockbed storage, latent heat storage, and cold water storage.

Hull, J. R.; Cole, R. L.; Hull, A. B.

1982-10-01

300

INDUCTION OF PROTEIN DROPLET (ALPHA(2-MU)-GLOBULIN) NEPHROPATHY IN MALE-RATS AFTER SHORT-TERM DOSAGE WITH 1,8-CINEOLE AND L-LIMONENE  

DEFF Research Database (Denmark)

As part of a series of short-term studies on peppermint oil constituents for their possible induction of the encephalopathy found with peppermint oil, 1,8-cineole and l-limonene were studied. Groups of 10 male Wistar rats were given 0, 500, or 1000 mg 1,8-cineole/kg body wt./day or 0, 800, or 1600 mg l-limonene/kg body wt./day for 2& days. 1,8-Cineole and l-limonene both induced accumulation of protein droplets containing alpha(2 mu)-globulin in proximal tubular epithelial cells in male rats. These results suggest that both 1,8-cineole and I-limonene possibly belong to the group of chemicals characterized by their induction of excessive alpha(2 mu)-globulin accumulation. Neither of the 2 substances induced encephalopathy.

Kristiansen, E.; Madsen, Charlotte Bernhard

1995-01-01

301

Sex hormone-binding globulin and androgen levels in immigrant and British-born premenopausal British Pakistani women : evidence of early life influences ?  

OpenAIRE

In women raised insulin levels are associated with low sex hormone-binding globulin (SHBG) and high androgen levels, which are in turn linked to infertility. Since insulin resistance and hyperinsulinaemia are major health problems for South Asians living in western countries we predicted that British Pakistani women would have low SHBG and raised androgen levels compared to European women. Given low birth weights in Pakistan, and known links between low birth weight and insulin resistance i...

Pollard, T. M.; Unwin, N. C.; Fischbacher, C. M.; Chamley, J. K.

2006-01-01

302

Sex hormone binding globulin and risk factors for breast cancer in a population of normal women who had never used exogenous sex hormones.  

OpenAIRE

Sex hormone binding globulin (SHBG) concentrations were measured by immunoradiometric assay in serum samples from 1,221 healthy female volunteers aged 34-79 who had never used oral contraceptives or hormone replacement therapy, had no history of thyroid disease or cancer, and had not used any drugs known to influence SHBG in the 14 days preceding blood sampling. There were 616 premenopausal and 605 naturally postmenopausal women. In both premenopausal and postmenopausal women, SHBG decreased ...

Moore, J. W.; Key, T. J.; Bulbrook, R. D.; Clark, G. M.; Allen, D. S.; Wang, D. Y.; Pike, M. C.

1987-01-01

303

Less Graft-Versus-Host Disease after Rabbit Antithymocyte Globulin Conditioning in Unrelated Bone Marrow Transplantation for Leukemia and Myelodysplasia: Comparison with Matched Related Bone Marrow Transplantation  

OpenAIRE

One of the major drawbacks for unrelated donor (UD) bone marrow transplantation (BMT) is graft-versus-host disease (GVHD). Despite results from randomized trials, antithymocyte globulin (ATG) is not routinely included for GVHD prophylaxis in UD BMT by many centers. One of ways to demonstrate the usefulness of rabbit ATG in UD BMT is to evaluate how its results approximate to those observed in matched related (MRD) BMT. Therefore, we compared the outcomes between UD BMT with rabbit ATG (Thymog...

Atta, Elias Hallack; Oliveira, Danielli Cristina Muniz; Bouzas, Luis Fernando; Nucci, Ma?rcio; Abdelhay, Eliana

2014-01-01

304

Interaction of blood sex steroid-binding globulin-steroid complexes with the plasma membranes of cells of the human decidual endometrium  

International Nuclear Information System (INIS)

The plasma membranes of cells of the decidual tissue specifically bind complexes of the sex steroid-binding globulin (SBG) of the blood with estrogens (estradiol, estriol, estrone) and the pharmacological agent danazol but do not interact with SBG-testosterone and SBG-dihydrotestosterone complexes. The selectivity of the interaction of SBG-steroid complexes with the cell membranes of the decidual tissue confirms the hypothesis of an active role of SBG in the action of steroids on this target tissue

305

Intravenous immune globulin (10% caprylate-chromatography purified) for the treatment of chronic inflammatory demyelinating polyradiculoneuropathy (ICE study): a randomised placebo-controlled trial  

OpenAIRE

Background: Short-term studies suggest that intravenous immunoglobulin might reduce disability caused by chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) but long-term effects have not been shown. We aimed to establish whether 10% caprylate-chromatography purified immune globulin intravenous (IGIV-C) has short-term and long-term benefit in patients with CIDP. Methods: 117 patients with CIDP who met specific neurophysiological inflammatory neuropathy cause and treatment (INCAT)...

Hughes, R. A. C.; Donofrio, P.; Bril, V.; Dalakas, M. C.; Deng, C.; Hanna, K.; Hartung, H. P.; Latov, N.; Merkies, I. S. J.; Doorn, P. A.

2008-01-01

306

The additionally glycosylated variant of human sex hormone-binding globulin (SHBG) is linked to estrogen-dependence of breast cancer.  

OpenAIRE

Sex Hormone-Binding Globulin (SHBG), the plasma carrier for androgens and estradiol, inhibits the estradiol-induced proliferation of breast cancer cells through its membrane receptor, cAMP, and PKA. In addition, the SHBG membrane receptor is preferentially expressed in estrogen-dependent (ER+/PR+) breast cancers which are also characterized by a lower proliferative rate than tumors negative for the SHBG receptor. A variant SHBG with a point mutation in exon 8, causing an aminoacid substitutio...

Biglia, Nicoletta; Frairia, Roberto; Sismondi, Piero

1999-01-01

307

The control of progesterone receptor expression in MCF-7 breast cancer cells: effects of estradiol and sex hormone-binding globulin (SHBG).  

OpenAIRE

Estradiol controls the gene transcription and expression of many proteins in breast cancer cells, like the progesterone receptor, PR, that is up-regulated by the hormone. Moreover, estradiol is one of the crucial factors inducing the proliferation of breast cancer cells. Sex Hormone-Binding Globulin (SHBG), the plasma carrier for both estradiol and androgens, inhibits the estradiol-induced growth of MCF-7 cells (estrogen-dependent breast cancer cells), through its membrane receptor (SHBG-R), ...

Frairia, Roberto

2001-01-01

308

Photon storage cavities  

International Nuclear Information System (INIS)

A general analysis is presented of a photon storage cavity, coupled to free-electron laser (FEL) cavity. It is shown that if the coupling between the FEL cavity and the storage cavity is unidirectional (for example, a ring resonator storage cavity) then storage is possible, but that if the coupling is bi-directional then storage is not possible. Parameters are presented for an infra-red FEL storage cavity giving an order of magnitude increase in the instantaneous photon power within the storage cavity. 4 refs., 3 figs

309

Mass storage management system  

International Nuclear Information System (INIS)

The author describes the application that the author developed during the time the author worked for Thomas Jefferson National Accelerator Facility, which creates an integrated storage environment. The application is built as a layer on top of the current storage manager (OSM), hiding details from the end user and manages access to all storage elements. A simple set of commands allows users to manipulate and move data between all storage elements of the system as well as to storage devices on remote machines

310

Methyl isobutyl ketone (MIBK) induction of ?2u-globulin nephropathy in male, but not female rats  

International Nuclear Information System (INIS)

Male F-344 rats were administered corn oil (vehicle control), d-limonene (positive control, 300 mg/kg), or MIBK (1000 mg/kg) and female F-344 rats corn oil (vehicle control) or MIBK for 10 consecutive days by oral gavage. Approximately 24 h after the final dose the kidneys were excised and the left kidney prepared and evaluated for histological changes including protein (hyaline) droplet accumulation, immunohistochemical staining for ?2u-globulin (?2u), and proliferating cell nuclear antigen (PCNA) to quantitate renal cell proliferation. The right kidney was prepared for quantitation of total protein and ?2u using an ELISA. MIBK elicited an increase in protein droplets, accumulation of ?2u, and renal cell proliferation in male, but not female rats, responses characteristic of ?2u-mediated nephropathy. MIBK produced identical histopathological changes in the male rat kidney when compared to d-limonene, an acknowledged inducer of ?2u-nephropathy except that the grade of severity tended to be slightly lower with MIBK. MIBK did not induce any effects in female rats. Therefore, renal histopathology, along with the other measures of ?2u accumulation, provides additional weight of evidence to support the inclusion of MIBK in the category of chemicals exerting renal effects through a ?2u-nephropathy-mediated mode-of-action

311

A review of human anti-globulin antibody (HAGA, HAMA, HACA, HAHA) responses to monoclonal antibodies. Not four letter words  

International Nuclear Information System (INIS)

The United States Food and Drugs Administration (FDA) has approved unconjugated monoclonal antibodies (MAbs) for immunotherapy (IT) of B-cell lymphoma, breast cancer and acute myeloid leukemia. More recently, approval has been given for conjugated ZevalinTM (90yttrium ibritumomab tiuxetan, IDEC-Y2B8, Biogen Idec, Cambridge, MA) and BexxarTM (131I-tositumomab, Corixa, Corp., Seattle, WA and GlaxoSmithKline, Philadelphia, PA) antiCD20 MAns for use in radioimmunotherapy (RIT) of non-Hodgikin's lymphoma (NHL), thus redefining the standard care of cancer patients. Because of, and despite a lack of basis for concern about allergic reactions due to human antibody responses to these foreign proteins, essays were developed to determine HAGE (human anti-globulin antibody) levels that developed in patient sera following treatment with MAbs. Strategies were also devised to humanize MAbs and to temporarily block patient immune function with drugs in order to decrease the seroconversion rates, with considerable success. On the other hand, a survival advantage has been observed in some patients who developed a HAGA following treatment. This correlates with development of an anti-idiotype antibody cascade directed toward the MAbs used to treat these patients. What follows is a selective review of HAGA and its effect on cancer treatment over the past 2 decades

312

Biological half-life and transfer of maternal corticosteroid-binding globulin to amniotic fluid in the rabbit  

International Nuclear Information System (INIS)

Rabbit corticosteroid-binding globulin (CBG) from the serum of pregnant and nonpregnant females differs in terms of charge microheterogeneity, and both forms were, therefore, radiolabeled and injected iv into 23- to 27-day pregnant rabbits (n = 6) to assess their biological half-lives and possible transfer to the fetal compartment. After an initially rapid distribution phase, the serum half-lives of both forms of [125I]CBG were essentially identical (approximately 13 h) and did not vary at different gestational ages. There was also no difference in the transfer of either form of [125I]CBG from maternal to fetal compartments in any of the animals studied. Moreover, [125I]CBG showed no sign of degradation and retained its steroid-binding activity in fetal urine and amniotic fluid. Twenty-two hours after administration of [125I]CBG to rabbits (n = 2) at 23 days gestation, its mean level in fetal urine (7 cpm/microliter) and amniotic fluid (2.5 cpm/microliter) was much higher than that in fetal blood (0.6 cpm/microliter). More importantly, the specific activities of [125I]CBG in fetal urine and amniotic fluid were comparable to that in maternal serum, and approximately 2 orders of magnitude higher than that in fetal serum. Taken together, these results suggest that CBG in fetal urine and amniotic fluid is largely of maternal origin, and that maternal CBG crosses the fetal kidney preferentiallyey preferentially

313

[Normality of serum thyroid hormones, thyroxine binding globulin and thyroid stimulating hormone concentrations in normal adult men (author's transl)].  

Science.gov (United States)

The normality of serum thyroxine (T4), triiodothyronine (T3), reverse T3, thyroxine binding globulin (TBG) and thyroid stimulating hormone (TSH) concentrations were investigated by using normal probability paper. 107 healthy adult men (age 35-48) were carefully selected; their healthiness was evaluated by physical, biochemical and roentogenologic examinations as well as by intensive interview on their past history. All assays were simultaneously carried out. When each cumulative distribution was plotted on normal probability paper, the straight line was obtained in T4, T3 and TBG but in rT3 and TSH, the lineality was seen only when logarithmic normal probability paper was used. The normal ranges by conventional statistical calculation, i.e., mean +/-2 S.D. was compared with those obtained on normal probability paper. The normal ranges of T4, T3 and TBG agreed well each other, whereas in rT3 and TSH, normal ranges obtained on the probability paper shifted toward higher levels than those by statistical calculations. Thus, it is suggested that the normal ranges of rT3 and TSH should be defined either by statistical calculation of each result after logarithmic transformation or by using logarithmic probability paper. PMID:6773867

Konno, N

1980-03-01

314

Proteomic and genetic analysis of wheat endosperm albumins and globulins using deletion lines of cultivar Chinese Spring  

DEFF Research Database (Denmark)

Albumins and globulins from the endosperm of Triticum aestivum L. cv Chinese Spring (CS) were analysed to establish a proteome reference map for this standard wheat cultivar. Approximately, 1,145 Coomassie-stained spots were detected by two-dimensional gel electrophoresis (2DE), 410 of which were identified using mass spectrometry and data mining. Salt-soluble endosperm proteins from 67 CS deletion lines were also separated by 2DE (four gels per line). Image analysis of the 268 2DE gels as compared to the CS reference proteome allowed the detection of qualitative and quantitative variations in endosperm proteins due to chromosomal deletions. This differential analysis of spots allowed structural or regulatory genes, encoding 211 proteins, to be located on segments of the 21 wheat chromosomes. In addition, variance analysis of quantitative variations in spot volume showed that the expression of 391 proteins is controlled by one or more chromosome bins with 262 significant increases and 196 significant decreases in spot volume. The spot volume of several proteins was increased or decreased by numerous chromosomal regions and homoeologous-like regulation was revealed for some proteins. Quantitative or qualitative variation in a total of 386 proteins was influenced by genes assigned to at least one chromosomal region, while 66 % of all stained proteins were not found to be influenced by chromosome bins. Proteomics of deletion lines can, therefore, be used to simultaneously analyse the composition and genetics of a complex tissue, such as the wheat endosperm.

Merlino, Marielle; Bousbata, Sabrina

2012-01-01

315

Characterization and quantification of corticosteroid-binding globulin in a southern toad, Bufo terrestris, exposed to coal-combustion-waste  

Energy Technology Data Exchange (ETDEWEB)

Corticosteroid-binding globulin (CBG) is a plasma protein that binds corticosterone and may regulate access of hormone to tissues. The role of CBG during a stress response is not clear. In this study, southern toads, Bufo terrestris, were exposed to a chronic pollutant (coal-combustion-waste), to determine changes in CBG and free corticosterone levels. Since toads exposed to chronic pollutants in previous studies did not exhibit the predicted changes in metabolic rate and mass, but did experience a significant elevation in total corticosterone, we hypothesized that CBG would likewise increase and thus, mitigate the effects of a chronic (i.e. 2 months) pollutant stressor. To conduct this study, we first characterized the properties of CBG in southern toads. After characterization, we monitored the changes in CBG, total corticosterone, and free corticosterone in male toads that were exposed to either coal-combustion-waste or control conditions. CBG increased in all groups throughout the experiment. Total corticosterone, on the other hand, was only significantly elevated at four weeks of exposure to coal-combustion-waste. The increase in CBG did not parallel the increase in total corticosterone; as a result, free corticosterone levels were not buffered by CBG, but showed a peak at four weeks similar to total corticosterone. This finding indicates that, in this species, CBG may not provide a protective mechanism during long-term pollution exposure.

Ward, C.K.; Fontes, C.; Breuner, C.W.; Mendonca, M.T. [Auburn University, Auburn, AL (USA). Dept. of Biological Science

2007-05-15

316

Induction of specific unresponsiveness to heart allografts in mongrel dogs treated with total lymphoid irradiation and antithymocyte globulin  

Energy Technology Data Exchange (ETDEWEB)

The survival of heterotopic heart allografts was determined in mongrel dogs treated with total lymphoid irradiation (TLI) alone or in combination with other immunosuppressive agents. TLI alone (total dose, 1800 rad) minimally prolonged graft survival as compared with untreated controls. However, marked synergy was observed when TLI was combined with a 10-day post-transplant course of rabbit anti-dog thymocyte globulin (ATG). Approximately 40% of recipients given TLI and ATG showed specific unresponsiveness, as judged by the lack of rejection on serial biopsies for more than 1 year and the prompt rejection of third party hearts. The addition of post-transplant azathioprine (90 to 180 days) to the TLI and ATG regimen increased the mortality of recipients and reduced the fraction of dogs showing specific unresponsiveness. Infusion of donor bone marrow cells at the time of heart transplantation failed to induced specific unresponsiveness in recipients given TLI alone or TLI in combination with post-transplant methotrexate, cyclosporine A, or ATG. The results indicate that the combination of TLI and a brief course of ATG without marrow transplantation was the most effective regimen for the induction of specific unresponsiveness in mongrel dogs.

Strober, S.; Modry, D.L.; Hoppe, R.T.; Pennock, J.L.; Bieber, C.P.; Holm, B.I.; Jamieson, S.W.; Stinson, E.B.; Schroder, J.; Suomalainen, H.; Kaplan, H.S.

1984-02-01

317

Electroimmunoassay of sex hormone binding globulin. Enhanced sensitivity by autoradiography using A-ring /sup 125/I-17-. beta. -oestradiol  

Energy Technology Data Exchange (ETDEWEB)

Four different /sup 125/I-iodinated steroids were tested for their binding to human sex hormone binding globulin (SHBG) using an electrophoretic technique. 17-..beta..-oestradiol iodinated in its A-ring bound with high affinity to SHBG. This radioactive steroid was used to increase the sensitivity of the electroimmunoassay of SHBG by adding the steroid to the samples before electroimmunoassay. The radioactive steroid incorporated into the immunoprecipitates could be observed by autoradiography. The sensitivity of the assay, which employed a rabbit antiserum against purified human SHBG and was standardized with pure SHBG, was about 0.2 mg/1. The coefficient of variation within and between assays was 2.4% and 2.6% respectively, for values within the normal range. The mean SHBG concentration in healthy regularly menstruating women was 3.50 +- 0.74 (SD) mg/1 when measured in plasma, and 3.78 +- 0.80 mg/1 when measured in serum. The corresponding mean concentrations in healthy men were 2.26 +- 0.45 and 2.44 +- 0.49 mg/1. The modified electroimmunoassay described is a simple modification, which increases the sensitivity sufficiently to permit reliable quantification of SHBG over the entire range of concentration which could be relevant in clinical practice.

Fernlund, P.; Kjellberg, M. (Lund Univ. (Sweden). Dept. of Clinical Chemistry); Gershagen, S.; Rannevik, G. (Fertility Research Inst., Malmoe (Sweden))

1984-02-01

318

Total lymphoid irradiation-antithymocyte globulin conditioning and allogeneic transplantation for patients with myelodysplastic syndromes and myeloproliferative neoplasms.  

Science.gov (United States)

Allogeneic hematopoietic cell transplantation (allo HCT) is the only curative therapy for the myelodysplastic syndromes (MDS) and myeloproliferative neoplasms (MPN), but treatment toxicity has been a barrier to its more widespread use. The nonmyeloablative regimen of total lymphoid irradiation (TLI) and antithymocyte globulin (ATG) permits the establishment of donor hematopoiesis necessary for the graft-versus-malignancy effect and is protective against acute graft-versus-host disease (aGVHD), but it has minimal direct cytotoxicity against myeloid diseases. We explored the use of TLI-ATG conditioning to treat 61 patients with allo HCT for MDS (n = 32), therapy-related myeloid neoplasms (n = 15), MPN (n = 9), and chronic myelomonocytic leukemia (n = 5). The median age of all patients was 63 years (range, 50 to 73). The cumulative incidence of aGVHD grades II to IV was 14% (95% confidence interval [CI], 4% to 23%) and for grades III to IV, 4% (95% CI, 0 to 9%), and it did not differ between patients who received allografts from related or unrelated donors. The cumulative incidence of nonrelapse mortality (NRM) at 100 days, 12 months, and 36 months was 0%, 7%, and 11%. Overall survival and progression-free survival were 41% (95% CI, 29% to 53%) and 35% (95% CI, 23% to 48%), respectively. The safety and tolerability of TLI-ATG, as exemplified by its low NRM, provides a foundation for further risk-adapted or prophylactic interventions to prevent disease progression. PMID:24607552

Benjamin, Jonathan; Chhabra, Saurabh; Kohrt, Holbrook E; Lavori, Philip; Laport, Ginna G; Arai, Sally; Johnston, Laura; Miklos, David B; Shizuru, Judith A; Weng, Wen-Kai; Negrin, Robert S; Lowsky, Robert

2014-06-01

319

Biological half-life and transfer of maternal corticosteroid-binding globulin to amniotic fluid in the rabbit  

Energy Technology Data Exchange (ETDEWEB)

Rabbit corticosteroid-binding globulin (CBG) from the serum of pregnant and nonpregnant females differs in terms of charge microheterogeneity, and both forms were, therefore, radiolabeled and injected iv into 23- to 27-day pregnant rabbits (n = 6) to assess their biological half-lives and possible transfer to the fetal compartment. After an initially rapid distribution phase, the serum half-lives of both forms of ({sup 125}I)CBG were essentially identical (approximately 13 h) and did not vary at different gestational ages. There was also no difference in the transfer of either form of ({sup 125}I)CBG from maternal to fetal compartments in any of the animals studied. Moreover, ({sup 125}I)CBG showed no sign of degradation and retained its steroid-binding activity in fetal urine and amniotic fluid. Twenty-two hours after administration of ({sup 125}I)CBG to rabbits (n = 2) at 23 days gestation, its mean level in fetal urine (7 cpm/microliter) and amniotic fluid (2.5 cpm/microliter) was much higher than that in fetal blood (0.6 cpm/microliter). More importantly, the specific activities of ({sup 125}I)CBG in fetal urine and amniotic fluid were comparable to that in maternal serum, and approximately 2 orders of magnitude higher than that in fetal serum. Taken together, these results suggest that CBG in fetal urine and amniotic fluid is largely of maternal origin, and that maternal CBG crosses the fetal kidney preferentially.

Seralini, G.E.; Underhill, C.M.; Smith, C.L.; Nguyen, V.T.; Hammond, G.L. (Univ. of Western Ontario at Victoria Hospital, London (Canada))

1989-09-01

320

In vitro model for induction of immunologic unresponsiveness to turkey. gamma. -globulin in primed mouse spleen cells. [Gamma radiation  

Energy Technology Data Exchange (ETDEWEB)

Unresponsiveness induced to turkey ..gamma..-globulin (TGG) in cultures of TGG-primed spleen cells by incubation with high concentrations of soluble TGG (sTGG) was shown to involve a state of active suppression. Upon transfer to secondary cultures of primed spleen cells stimulated with an optimal dose of TGG-conjugated erythrocytes, such tolerant spleen cells were able to actively inhibit a secondary plaque-forming cell response to TGG in these cultures. Almost complete inhibition was observed with a tolerant cell to primed cell ratio of as low as 0.1. The suppression was antigen specific in that tolerant spleen cells which were suppressive for the secondary TGG response were unable to inhibit a primary response to sheep erthrocytes. T cells were shown to be required for the suppressor effect, in that (i) suppressor activity could be removed by complement-mediated lysis with an anti-Thy 1.2 antiserum and (ii) suppressor activity was retained in the effluent fraction after passage of suppressor spleen cells over a nylon wool column. Induction of the T-cell suppressor activity was found to be associated with a loss of T-cell helper activity within the TGG-pulsed cell population. The presence of adherent cells was not required for induction of suppressor activity. Furthermore, the suppressor effect was found to be resistant to 1000 R of ..gamma.. irradiation.

Sieckmann, D.G.; Chiller, J.M.; Weigle, W.O.

1979-02-01

321

Enhanced efficacy of cidofovir combined with vaccinia immune globulin in treating progressive cutaneous vaccinia virus infections in immunosuppressed hairless mice.  

Science.gov (United States)

Treatment of progressive vaccinia in individuals has involved antiviral drug therapy such as cidofovir (CDV), brincidofovir, and/or tecovirimat combined with vaccinia immune globulin (VIG). VIG is costly and the supply is limited, thus, sparing of VIG during treatment is an important objective. VIG sparing was modeled in immunosuppressed mice by maximizing treatment benefits using CDV combined with VIG, by determining effective treatments that delayed the time to death, reduced cutaneous lesion severity, and/or decreased tissue viral titers. SKH-1 hairless mice immunosuppressed with cyclophosphamide and hairless SCID mice (SHO strain) were infected cutaneously with vaccinia virus. Monotherapy, dual combinations (CDV plus VIG), or triple therapy (topical CDV, parenteral CDV, and VIG) were initiated two days post-infection and were given every 3-4 days through day 11. Efficacy assessment included survival, cutaneous lesion severity, and viral titers. Delays in time to death and reduction in lesion severity occurred, with the following order of efficacy seen: triple therapy > double combinations (CDV plus VIG, or CDV topical plus CDV parenteral) > VIG alone. Parenteral administration of CDV or VIG was necessary to suppress virus titers in internal organ (liver, lung, and spleen). Skin viral titers were significantly reduced only by the triple therapy. The greatest efficacy was achieved by triple therapy. In humans this regimen should translate to a faster cure rate, thus sparing the amount of VIG used for treatment. PMID:25385098

Smee, Donald F; Dagley, Ashley; Downs, Brittney; Hagloch, Joseph; Tarbet, E Bart

2014-11-10

322

The Pretreatment Albumin to Globulin Ratio Has Predictive Value for Long-Term Mortality in Nasopharyngeal Carcinoma  

Science.gov (United States)

Background Low serum albumin is predictive of poor survival in nasopharyngeal carcinoma (NPC). We evaluated the ability of the pretreatment albumin/globulin ratio (AGR) to predict long-term mortality in patients with NPC. Methods This retrospective study examined an unselected cohort of 694 patients with NPC who had documented pretreatment total serum protein and serum albumin levels (ALB). AGR was calculated as [AGR?=?ALB/(total serum protein - ALB)]. Survival analysis was used to evaluate the predictive value of AGR. Results Multivariate analysis demonstrated that a low pretreatment serum AGR (<1.4) was an independent predictor of poor OS (P ?=?0.029) and DMFS (P ?=?0.033). A low AGR was significantly associated with advanced stage disease (P<0.001), high white blood cell count (P ?=?0.033), high neutrophil count (P ?=?0.047), high total serum protein (P<0.001) and low ALB (P<0.001). Conclusion The pretreatment AGR may represent a simple, potentially useful predictive biomarker for evaluating the long-term prognosis of patients with undifferentiated NPC. PMID:24718309

Du, Xiao-Jing; Tang, Ling-Long; Mao, Yan-Ping; Sun, Ying; Zeng, Mu-Sheng; Kang, Tie-Bang; Jia, Wei-Hua; Lin, Ai-Hua; Ma, Jun

2014-01-01

323

Repair of UV-induced DNA damage in aplastic anaemia: Changes after treatment with antilymphocyte globulin (ALG)  

Energy Technology Data Exchange (ETDEWEB)

The extent of DNA-repair induced by UV-C irradiation was measured in peripheral unstimulated lymphocytes of 24 patients with aplastic anaemia at different stages of disease and compared with the results obtained in 92 controls. As parameter of the DNA-repair synthesis, the incorporation of (/sup 3/H)thymidine in the presence of 2 mmol/l hydroxyurea (HU) was taken. Of 19 patients tested after treatment with antilymphocyte globulin (ALG), 5 were in complete autologous haemopoietic remission, defined as > 1000 granulocytes/mm/sup 3/, > 100 000 platelets/mm/sup 3/ and a nontransfused haemoglobin value > 10 g%. 14 patients were in partial remission, defined as improvement of haemopoietic function, not meeting the criteria for complete remission. 4/5 patients in complete remission had normal DNA-repair synthesis, compared to 4/14 patients in partial remission. In 92 controls, a normal level was found in 70 cases. In 4/5 patients examined at diagnosis and at various intervals after ALG-treatment, DNA-repair synthesis was low at diagnosis. It increased after therapy and paralleled improvement of haemopoietic function to some extent. It is suggested that in aplastic anaemia there are different populations of lymphocytes with differing DNA-repair capacity; ALG treatment seems to favour expansion of the normal population, which is associated with improvement of haemopoietic function.

Kovacs, E.; Nissen, C.; Speck, B.; Signer, E.

1988-01-01

324

Differential expression and elution behavior of basic 7S globulin among cultivars under hot water treatment of soybean seeds.  

Science.gov (United States)

Basic 7S globulin (Bg7S), which accumulates in mature soybean (Glycine max) seeds, is an extracellular matrix protein. A large amount of Bg7S is synthesized de novo and is eluted from soybean seeds when immersed in 50-60°C water (hot water treatment, HWT). However, the Bg7S elution mechanism remains unclear. Under HWT, the seeds probably undergo heat stress and flooding stress. To obtain fundamental knowledge related to how Bg7S is eluted from hot-water-treated seeds, this study compared Bg7S elution among soybean cultivars having different flooding tolerance during pre-germination. The amounts of Bg7S eluted from seeds varied significantly among cultivars. Elution was suppressed by seed coats regarded as preventing the leakage of seed contents by rapid water imbibition. Furthermore, Bg7S expression levels differed among cultivars, although the difference did not result from any variation in Bg7S promoter sequences. However, the expression levels of Bg7S under HWT were not associated with the flooding tolerance level. Immunoelectron microscopy revealed that the Bg7S accumulated in the intercellular space of hot-water-treated seeds. Plasma membrane shrinkage was observed. The main proteins eluted from seeds under HWT were located in the extracellular space. This study clarified the mechanism of Bg7S elution from seeds under HWT. PMID:24331980

Fujiwara, Keigo; Cabanos, Cerrone; Toyota, Kenji; Kobayashi, Yasunori; Maruyama, Nobuyuki

2014-06-01

325

A review of human anti-globulin antibody (HAGA, HAMA, HACA, HAHA) responses to monoclonal antibodies. Not four letter words  

Energy Technology Data Exchange (ETDEWEB)

The United States Food and Drugs Administration (FDA) has approved unconjugated monoclonal antibodies (MAbs) for immunotherapy (IT) of B-cell lymphoma, breast cancer and acute myeloid leukemia. More recently, approval has been given for conjugated ZevalinTM ({sup 9}0yttrium ibritumomab tiuxetan, IDEC-Y2B8, Biogen Idec, Cambridge, MA) and BexxarTM ({sup 1}31I-tositumomab, Corixa, Corp., Seattle, WA and GlaxoSmithKline, Philadelphia, PA) antiCD20 MAns for use in radioimmunotherapy (RIT) of non-Hodgikin's lymphoma (NHL), thus redefining the standard care of cancer patients. Because of, and despite a lack of basis for concern about allergic reactions due to human antibody responses to these foreign proteins, essays were developed to determine HAGE (human anti-globulin antibody) levels that developed in patient sera following treatment with MAbs. Strategies were also devised to humanize MAbs and to temporarily block patient immune function with drugs in order to decrease the seroconversion rates, with considerable success. On the other hand, a survival advantage has been observed in some patients who developed a HAGA following treatment. This correlates with development of an anti-idiotype antibody cascade directed toward the MAbs used to treat these patients. What follows is a selective review of HAGA and its effect on cancer treatment over the past 2 decades.

Mirick, G. R.; Bradt, B. M.; Denardo, S. J.; Denardo, G. L. [Calfornia Univ., Sacramento (United States). Davis Medical Center

2004-12-01

326

Immunosuppressive therapy with horse anti-thymocyte globulin and cyclosporine as treatment for fulminant aplastic anemia in children.  

Science.gov (United States)

Patients with severe aplastic anemia (SAA) and an absolute neutrophil count (ANC) of 0 typically have fatal outcomes. We defined fulminant AA (FAA) as ANC?=?0 for at least 2 weeks prior to and after immunosuppressive therapy (IST). We analyzed the outcomes of 35 children with FAA among 288 children who enrolled in a prospective study for AA (AA-97 study). AA was classified as FAA (n?=?35), very SAA (vSAA; n?=?129), or SAA (n?=?124). All of the children received the IST with horse anti-thymocyte globulin (ATG) and cyclosporine (CsA). A significantly lower response rate at 6 months was seen in children with FAA when compared to those with vSAA or SAA (40.0, 63.6, and 63.7 %, respectively; p?=?0.027). Of 20 nonresponder patients in the FAA group, 11 were rescued by alternative donor transplantation, and 5 patients showed a late response after 6 months. Consequently, no significant difference was noted in overall survival when comparing the FAA, vSAA, and SAA groups (88.5, 95.8, and 96.8 %). These findings indicate that IST with ATG and CsA is justified as a first-line treatment for children with FAA who lack a human leukocyte antigen-matched sibling donor. PMID:24337487

Yagasaki, Hiroshi; Shichino, Hiroyuki; Ohara, Akira; Kobayashi, Ryoji; Yabe, Hiromasa; Ohga, Shouichi; Hamamoto, Kazuko; Ohtsuka, Yoshitoshi; Shimada, Hiroyuki; Inoue, Masami; Muramatsu, Hideki; Takahashi, Yoshiyuki; Kojima, Seiji

2014-05-01

327

Pre-transplant antithymocyte globulin has increased efficacy in controlling donor-reactive memory T cells in mice  

Science.gov (United States)

Antibody-mediated lymphocyte depletion is frequently used as induction therapy in sensitized transplant patients. Although T cells with an effector/memory phenotype remain detectable after lymphoablative therapies in human transplant recipients, the role of pre-existing donor-reactive memory in reconstitution of the T cell repertoire and induction of alloimmune responses following lymphoablation is poorly understood. We show in a mouse cardiac transplantation model that anti-donor immune responses following treatment with rabbit anti-mouse thymocyte globulin (mATG) were dominated by T cells derived from the pre-existing memory compartment. Administration of mATG one week prior to transplantation (pre-TP) was more efficient in targeting pre-existing donor-reactive memory T cells, inhibiting overall anti-donor T cell responses, and prolonging heart allograft survival than the commonly used treatment at the time of transplantation (peri-TP). The failure of peri-TP mATG to control anti-donor memory responses was due to faster recovery of pre-existing memory T cells rather than their inefficient depletion. This rapid recovery did not depend on T cell specificity for donor alloantigens suggesting an important role for posttransplant inflammation in this process. Our findings provide insights into the components of the alloimmune response remaining after lymphoablation and may help guide the future use of ATG in sensitized transplant recipients. PMID:23331999

Ayasoufi, Katayoun; Yu, Hong; Fan, Ran; Wang, Xi; Williams, John; Valujskikh, Anna

2014-01-01

328

Induction of suppressive allogeneic regulatory T cells via rabbit antithymocyte polyclonal globulin during homeostatic proliferation in rat kidney transplantation.  

Science.gov (United States)

Experimental studies have shown that rabbit antithymocyte polyclonal globulin (ATG) can expand human CD4+CD25++Foxp3+ cells (Tregs). We investigated the major biological effects of a self-manufactured rabbit polyclonal anti-rat thymoglobulin (rATG) in vitro, as well as its effects on different peripheral T-cell subsets. Moreover, we evaluated the allogeneic suppressive capacity of rATG-induced Tregs in an experimental rat renal transplant model. Our results show that rATG has the capacity to induce apoptosis in T lymphocyte lymphocytes as a primary mechanism of T-cell depletion. Our in vivo studies demonstrated a rapid but transient cellular depletion of the main T cell subsets, directly proportional to the rATG dose used, but not of the effector memory T cells, which required significantly higher rATG doses. After rATG administration, we observed a significant proliferation of Tregs in the peripheral blood of transplanted rats, leading to an increase in the Treg/T effector ratio. Importantly, rATG-induced Tregs displayed a strong donor-specific suppressive capacity when assessed in an antigen-specific allogeneic co-culture. All of these results were associated with better renal graft function in rats that received rATG. Our study shows that rATG has the biological capacity immunomodulatory to promote a regulatory alloimmune milieu during post-transplant homeostatic proliferation. PMID:25208307

Valdez-Ortiz, Rafael; Bestard, Oriol; Llaudó, Inés; Franquesa, Marcella; Cerezo, Gema; Torras, Joan; Herrero-Fresneda, Inmaculada; Correa-Rotter, Ricardo; Grinyó, Josep M

2015-01-01

329

Regulation of plasma corticosteroid-binding globulin in adult cynomolgus monkey (Macaca fascicularis) during different reproductive states.  

Science.gov (United States)

The plasma concentration of the corticosteroid-binding globulin (mCBG) has been measured in Macaca fascicularis, during different stages of reproduction and under hormonal treatments. The mCBG level was determined by a specific electroimmunoassay. There was no difference between females in the follicular phase and intact males; mCBG concentrations were respectively (mean +/- SEM) 469 +/- 53 and 443 +/- 25.6 nmol/l. The mCBG levels levels were similar during both the luteal (469 +/- 33.5 nmol/l) and the follicular phase (469 +/- 53 nmol/l). Compared to intact males, the mCBG levels were higher (P less than 0.05) in castrated males (527 +/- 6.6 nmol/l). During gestation, no systematic variations were found and the mCBG levels were not statistically different from the values found during the follicular phase. When estradiol benzoate was administered to castrated animals, the mCBG concentrations increased rapidly. In contrast, the values were reduced slightly by testosterone treatment. The sex-steroid action on the mCBG levels was discussed and compared with the mSBP levels. We question also, the mechanisms involved in the regulation of the mCBG levels during pregnancy. PMID:2214775

Kottler, M L; Domingo, M; Tardivel-Lacombe, J; Egloff, M; Dang, C D; Degrelle, H

1990-08-28

330

Human serum does not contain a high affinity estrogen-binding glycoprotein different from sex hormone-binding globulin.  

Science.gov (United States)

The mannoglycoprotein fraction obtained by Concanavalin-A chromatography of human serum binds both androgens and estrogens with high affinity. Sex hormone-binding globulin (SHBG) is a component of this fraction that binds both steroids, but the fraction may contain another component that binds only estrogen. We used several chromatographic methods to ascertain whether the estradiol-binding properties of the mannoglycoprotein fraction could be attributed to SHBG or to SHBG and the putative estrogen-binding protein. DEAE-trisacryl, chromatofocusing, and anti-SHBG-immunoglobulin Sepharose chromatography resulted in coelution of the androgen- and estrogen-binding activities. SHBG purified by ligand affinity chromatography as well as immunoaffinity-purified SHBG had estradiol-binding properties similar to those of the crude mannoglycoprotein preparation. These data strongly suggest that 1) SHBG is the only estradiol-binding protein in the mannoglycoprotein fraction obtained by Concanavalin-A chromatography of human serum, and 2) the putative estradiol-binding protein of serum is most likely SHBG. PMID:2715292

Reny, J L; Soto, A M

1989-05-01

331

The (TAAAA)n polymorphism of sex hormone-binding globulin gene is not associated with testicular maldescent.  

Science.gov (United States)

The aim of this family-based study was to investigate the potential association/genetic linkage of the (TAAAA)n polymorphism of sex hormone-binding globulin gene proximal promoter with testicular maldescent (TMD). Genomic DNA was extracted from the peripheral blood of 487 subjects (174 index families): (i) 180 children with all phenotypes of TMD, (ii) 307 parents (156 mothers and 151 fathers). Conventional polymerase chain reaction amplification products were electrophoresed on 10% nondenaturating polyacrylamide gel and visualised by silver staining. After excluding ambiguous parental-child trios and most cases of index families with missing parental genotypes, 429 individuals were left for analysis: 138 completely typed nuclear families (five included a second affected child) and five child-parent couples (one parent missing). Eight fathers presented history of TMD, that is, a total of 156 cases with TMD were analysed. Alleles were analysed with the affected family-based control method and logistic regression-based extension of the transmission disequilibrium test for multiallelic loci. (?????)n polymorphism analysis revealed six alleles based on repeat numbers (n=5-10). No association/genetic linkage between the (TAAAA)n polymorphism and TMD was detected. Other factors should be investigated to potentially explain the genetic predisposition that seems to exist in at least a subgroup of these patients. PMID:22591465

Mamoulakis, C; Sofikitis, N; Tsounapi, P; Vlachopoulou, E; Chatzikyriakidou, A; Antypas, S; Tzortzakakis, D; Sofras, F; Takenaka, A; Georgiou, I

2013-02-01

332

Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes  

DEFF Research Database (Denmark)

Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (?20%), pauci-mannosidic (?40%), and complex forms (?40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants ?-1,2-xylose and ?-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectincontaining two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2 (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has the potential to serve as a model system for studying the role of seed proteins and their glycosylation in food allergy.

Dam, Svend Secher; Thaysen-Andersen, Morten

2013-01-01

333

Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background In legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius (L., also known as narrow-leaf lupin (NLL is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch. Results Genes encoding the seed storage proteins of NLL were characterised by sequencing cDNA clones derived from developing seeds. Four families of seed storage proteins were identified and comprised three unique ?, seven ?, two ? and four ? conglutins. This study added eleven new expressed storage protein genes for the species. A comparison of the deduced amino acid sequences of NLL conglutins with those available for the storage proteins of Lupinus albus (L., Pisum sativum (L., Medicago truncatula (L., Arachis hypogaea (L. and Glycine max (L. permitted the analysis of a phylogenetic relationships between proteins and demonstrated, in general, that the strongest conservation occurred within species. In the case of 7S globulin (? conglutins and 2S sulphur-rich albumin (? conglutins, the analysis suggests that gene duplication occurred after legume speciation. This contrasted with 11S globulin (? conglutin and basic 7S (? conglutin sequences where some of these sequences appear to have diverged prior to speciation. The most abundant NLL conglutin family was ? (56%, followed by ? (24%, ? (15% and ? (6% and the transcript levels of these genes increased 103 to 106 fold during seed development. We used the 16 NLL conglutin sequences identified here to determine that for individuals specifically allergic to lupin, all seven members of the ? conglutin family were potential allergens. Conclusion This study has characterised 16 seed storage protein genes in NLL including 11 newly-identified members. It has helped lay the foundation for efforts to use molecular breeding approaches to improve lupins, for example by reducing allergens or increasing the expression of specific seed storage protein(s with desirable nutritional properties.

Goggin Danica E

2011-04-01

334

Windows Azure data storage  

CERN Document Server

Make tools for storage accessible from Azure apps and any Internet connected app In Windows Azure Data Storage, Simon Hart of Microsoft's Windows Azure Center of Excellence Team gives you all the tools you need to provide storage that is accessible both from Windows Azure applications and any application connected to the Internet. The book starts with an overview of the fundamental concepts, and then quickly dives in to configuring, scenario mapping, security, backup, recovery, and maintenance of the various data storage options. Contents include: Data Storage OptionsLocal StorageBlob Stora

Johnson, Bruce

2013-01-01

335

Storage in Europe  

International Nuclear Information System (INIS)

Storage represents one of the key elements among the different modulation tools. How the problem of storage is put forward in Europe in front of the increasing uncertainty of the gas demand and prices? What are the policies implemented by storage facility operators? To what extend storage can amortize gas prices volatility or allow the market actors to take the best profit of this volatility? These are the questions debated at this workshop by four specialists of this domain. (J.S.)

336

Fuel storage systems  

Energy Technology Data Exchange (ETDEWEB)

Storage technologies are characterized for solid, liquid, and gaseous fuels. Emphasis is placed on storage methods applicable to Integrated Community Energy Systems based on coal. Items discussed here include standard practice, materials and energy losses, environmental effects, operating requirements, maintenance and reliability, and cost considerations. All storage systems were found to be well-developed and to represent mature technologies; an exception may exist for low-Btu gas storage, which could have materials incompatability.

Donakowski, T.D.; Tison, R.R.

1979-08-01

337

Abstract Storage Devices  

OpenAIRE

A quantum storage device differs radically from a conventional physical storage device. Its state can be set to any value in a certain (infinite) state space, but in general every possible read operation yields only partial information about the stored state. The purpose of this paper is to initiate the study of a combinatorial abstraction, called abstract storage device (ASD), which models deterministic storage devices with the property that only partial information about...

Koenig, Robert; Maurer, Ueli; Tessaro, Stefano

2007-01-01

338

Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in english Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and identified component of the sorting machinery. Immature sunflow [...] er seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, ?-TIP, ?-TIP, BiP, and two proteins that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with pea VSR BP80. Electronic micrographies of developing seeds show that the formation of electron dense aggregates starts in the endoplasmic reticulum, and that these aggregates are very abundant in the trans-Golgi apparatus, where release of dense vesicles happens. These vesicles contain a homolog of PV72 in their membranes. Storage proteins are also detected in multivesicular bodies whose membranes have reactivity with PV72 serum. All these results indicated that sunflower storage proteins are transported to protein storage vacuoles by a Golgi dependent pathway in a process in which homologous of PV72 are involved.

María Isabel, Molina; Marisa, Otegui; Silvana, Petruccelli.

2006-06-01

339

The role of sex hormone binding globulin (SHBG) in conversion of testosterone to 5 ?-dihydrotestosterone and androstenedione in skin from suprapubic area of women. Part 1. In vitro study with skin sections  

International Nuclear Information System (INIS)

The influence of sex hormone binding globulin (SHBG) on testosterone metabolism into 5 alpha-dihydrotestosterone and androstenedione in skin sections from the suprapubic area women of reproductive age was investigated. The SHBG was obtained by precipitating plasma proteins of women in the third trimester of pregnancy with ammonium sulphate. Steroids were isolated from the incubation medium by extraction with acetone and reextraction with ether and purified using column chromatography on aluminum oxide, thin layer chromatography (TLC) on silica gel and following formation of acetyl derivatives the thin layer chromatography on silica gel was again carried out. Individual recovery of androstenedione was calculated using radioisotope and the recovery of 5 alpha-dihydrotestosterone was determined with gas chromatography. Mean value of identified 5 alpha-dihydrotestosterone in the presence of inactive globulin was 52.77 fg/mg, whereas following the addition of active globulin the value decreased to 19.62 fg/mg. Mean value of identified androstenedione in preparations with inactive globulin (62.97 fg/mg), in the presence of active globulin was also reduced to 18.15 fg/mg. (author)

340

Production of the growth factors GM-CSF, G-CSF, and VEGF by human peripheral blood cells induced with metal complexes of human serum ? -globulin formed with copper or zinc ions.  

Science.gov (United States)

As it was established in our previous studies, the proteins of human serum ?-globulin fraction could interact with copper or zinc ions distributed in the periglobular space, form metal complexes, and become able to perform effector functions differing due to the conformational shifts from those mediated by them in native conformation of their Fc regions. In the present work we have evaluated ability of the ?-globulin metal complexes formed with copper or zinc ions in the conditions like to the physiological ones to induce production or to regulate induction in the culture of freshly isolated human peripheral blood cells (PBC) of granulocyte (G) and granulocyte-macrophage (GM) colony-stimulating factors (CSF) as well as of vascular endothelial growth factor (VEGF). The ?-globulin metal complexes formed with both copper and zinc ions were found to similarly reduce production of GM-CSF, G-CSF, and VEGF induced in normal human PBC cultures by the control ?-globulins or by copper and zinc ions used alone. In context of theory and practice of inflammation the properties of the ?-globulin metal complexes might impact the basic knowledge in search of novel approaches to anti-inflammatory drugs development. PMID:25104881

Cheknev, Sergey B; Apresova, Maria A; Moryakova, Nadezhda A; Efremova, Irina E; Mezdrokhina, Anna S; Piskovskaya, Lidya S; Babajanz, Alla A

2014-01-01

341

Production of the Growth Factors GM-CSF, G-CSF, and VEGF by Human Peripheral Blood Cells Induced with Metal Complexes of Human Serum ?-Globulin Formed with Copper or Zinc Ions  

Science.gov (United States)

As it was established in our previous studies, the proteins of human serum ?-globulin fraction could interact with copper or zinc ions distributed in the periglobular space, form metal complexes, and become able to perform effector functions differing due to the conformational shifts from those mediated by them in native conformation of their Fc regions. In the present work we have evaluated ability of the ?-globulin metal complexes formed with copper or zinc ions in the conditions like to the physiological ones to induce production or to regulate induction in the culture of freshly isolated human peripheral blood cells (PBC) of granulocyte (G) and granulocyte-macrophage (GM) colony-stimulating factors (CSF) as well as of vascular endothelial growth factor (VEGF). The ?-globulin metal complexes formed with both copper and zinc ions were found to similarly reduce production of GM-CSF, G-CSF, and VEGF induced in normal human PBC cultures by the control ?-globulins or by copper and zinc ions used alone. In context of theory and practice of inflammation the properties of the ?-globulin metal complexes might impact the basic knowledge in search of novel approaches to anti-inflammatory drugs development. PMID:25104881

Cheknev, Sergey B.; Apresova, Maria A.; Moryakova, Nadezhda A.; Efremova, Irina E.; Mezdrokhina, Anna S.; Piskovskaya, Lidya S.; Babajanz, Alla A.

2014-01-01

342

Genetic evidence that raised sex hormone binding globulin (SHBG) levels reduce the risk of type 2 diabetes  

DEFF Research Database (Denmark)

Epidemiological studies consistently show that circulating sex hormone binding globulin (SHBG) levels are lower in type 2 diabetes patients than non-diabetic individuals, but the causal nature of this association is controversial. Genetic studies can help dissect causal directions of epidemiological associations because genotypes are much less likely to be confounded, biased or influenced by disease processes. Using this Mendelian randomization principle, we selected a common single nucleotide polymorphism (SNP) near the SHBG gene, rs1799941, that is strongly associated with SHBG levels. We used data from this SNP, or closely correlated SNPs, in 27 657 type 2 diabetes patients and 58 481 controls from 15 studies. We then used data from additional studies to estimate the difference in SHBG levels between type 2 diabetes patients and controls. The SHBG SNP rs1799941 was associated with type 2 diabetes [odds ratio (OR) 0.94, 95% CI: 0.91, 0.97; P = 2 x 10(-5)], with the SHBG raising allele associated with reduced risk of type 2 diabetes. This effect was very similar to that expected (OR 0.92, 95% CI: 0.88, 0.96), given the SHBG-SNP versus SHBG levels association (SHBG levels are 0.2 standard deviations higher per copy of the A allele) and the SHBG levels versus type 2 diabetes association (SHBG levels are 0.23 standard deviations lower in type 2 diabetic patients compared to controls). Results were very similar in men and women. There was no evidence that this variant is associated with diabetes-related intermediate traits, including several measures of insulin secretion and resistance. Our results, together with those from another recent genetic study, strengthen evidence that SHBG and sex hormones are involved in the aetiology of type 2 diabetes.

Perry, John R B; Weedon, Michael N

2010-01-01

343

Genome Wide Association Identifies Common Variants at the SERPINA6/SERPINA1 Locus Influencing Plasma Cortisol and Corticosteroid Binding Globulin  

Science.gov (United States)

Variation in plasma levels of cortisol, an essential hormone in the stress response, is associated in population-based studies with cardio-metabolic, inflammatory and neuro-cognitive traits and diseases. Heritability of plasma cortisol is estimated at 30–60% but no common genetic contribution has been identified. The CORtisol NETwork (CORNET) consortium undertook genome wide association meta-analysis for plasma cortisol in 12,597 Caucasian participants, replicated in 2,795 participants. The results indicate that <1% of variance in plasma cortisol is accounted for by genetic variation in a single region of chromosome 14. This locus spans SERPINA6, encoding corticosteroid binding globulin (CBG, the major cortisol-binding protein in plasma), and SERPINA1, encoding ?1-antitrypsin (which inhibits cleavage of the reactive centre loop that releases cortisol from CBG). Three partially independent signals were identified within the region, represented by common SNPs; detailed biochemical investigation in a nested sub-cohort showed all these SNPs were associated with variation in total cortisol binding activity in plasma, but some variants influenced total CBG concentrations while the top hit (rs12589136) influenced the immunoreactivity of the reactive centre loop of CBG. Exome chip and 1000 Genomes imputation analysis of this locus in the CROATIA-Korcula cohort identified missense mutations in SERPINA6 and SERPINA1 that did not account for the effects of common variants. These findings reveal a novel common genetic source of variation in binding of cortisol by CBG, and reinforce the key role of CBG in determining plasma cortisol levels. In turn this genetic variation may contribute to cortisol-associated degenerative diseases. PMID:25010111

Direk, Nese; Lewis, John G.; Hammond, Geoffrey L.; Hill, Lesley A.; Anderson, Anna; Huffman, Jennifer; Wilson, James F.; Campbell, Harry; Rudan, Igor; Wright, Alan; Hastie, Nicholas; Wild, Sarah H.; Velders, Fleur P.; Hofman, Albert; Uitterlinden, Andre G.; Lahti, Jari; Räikkönen, Katri; Kajantie, Eero; Widen, Elisabeth; Palotie, Aarno; Eriksson, Johan G.; Kaakinen, Marika; Järvelin, Marjo-Riitta; Timpson, Nicholas J.; Davey Smith, George; Ring, Susan M.; Evans, David M.; St Pourcain, Beate; Tanaka, Toshiko; Milaneschi, Yuri; Bandinelli, Stefania; Ferrucci, Luigi; van der Harst, Pim; Rosmalen, Judith G. M.; Bakker, Stephen J. L.; Verweij, Niek; Dullaart, Robin P. F.; Mahajan, Anubha; Lindgren, Cecilia M.; Morris, Andrew; Lind, Lars; Ingelsson, Erik; Anderson, Laura N.; Pennell, Craig E.; Lye, Stephen J.; Matthews, Stephen G.; Eriksson, Joel; Mellstrom, Dan; Ohlsson, Claes; Price, Jackie F.; Strachan, Mark W. J.; Reynolds, Rebecca M.; Tiemeier, Henning; Walker, Brian R.

2014-01-01

344

Characterization and quantification of corticosteroid-binding globulin in a southern toad, Bufo terrestris, exposed to coal-combustion-waste.  

Science.gov (United States)

Corticosteroid-binding globulin (CBG) is a plasma protein that binds corticosterone and may regulate access of hormone to tissues. The role of CBG during a stress response is not clear. At least two hypotheses have been proposed: 1) CBG levels may increase in response to a stressor, thereby decreasing the amount of circulating free corticosterone, or 2) CBG levels may decline, making corticosterone available for its role in increased metabolic needs during stress. In this study, southern toads, Bufo terrestris, were exposed to a chronic pollutant (coal-combustion-waste), to determine changes in CBG and free corticosterone levels. Since toads exposed to chronic pollutants in previous studies did not exhibit the predicted changes in metabolic rate and mass, but did experience a significant elevation in total corticosterone, we hypothesized that CBG would likewise increase and thus, mitigate the effects of a chronic (i.e. 2 months) pollutant stressor. To conduct this study, we first characterized the properties of CBG in southern toads. Toad CBG has a K(d)=20.6+/-1.0 nM and a B(max)=332.2+/-5.1 nmol/L plasma. The rank order potencies for steroid inhibition of tritiated corticosterone are: dihydrotestosterone > corticosterone > progesterone=testosterone > estrogen=dexamethasone. After characterization, we monitored the changes in CBG, total corticosterone, and free corticosterone in male toads that were exposed to either coal-combustion-waste or control conditions. CBG increased in all groups throughout the experiment. Total corticosterone, on the other hand, was only significantly elevated at four weeks of exposure to coal-combustion-waste. The increase in CBG did not parallel the increase in total corticosterone; as a result, free corticosterone levels were not buffered by CBG, but showed a peak at four weeks similar to total corticosterone. This finding indicates that, in this species, CBG may not provide a protective mechanism during long-term pollution exposure. PMID:17428483

Ward, Chelsea K; Fontes, Cristiano; Breuner, Creagh W; Mendonça, Mary T

2007-05-15

345

Prediagnostic plasma testosterone, sex hormone binding globulin, IGF-I and hepatocellular carcinoma : Etiological factors or risk markers?  

DEFF Research Database (Denmark)

Elevated pre-diagnostic testosterone and insulin-like growth factor-I (IGF-I) concentrations have been proposed to increase risk of hepatocellular carcinoma (HCC). However, the metabolism of these hormones is altered as a consequence of liver damage and they may have clinical utility as HCC risk markers. A case-control study was nested within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort and included 125 incident HCC cases and 247 individually matched controls. Testosterone, sex hormone binding globulin (SHBG) and IGF-I were analyzed by immunoassays. Odds ratios (OR) and 95% confidence intervals (CI) were estimated by conditional logistic regression. The area under the receiver operating curves (AUC) was calculated to assess HCC predictive ability of the tested models. After adjustments for epidemiological variables (body mass index, smoking, ethanol intake, hepatitis and diabetes) and liver damage (a score based on albumin, bilirubin, aspartate aminotransaminase, alanine aminotransaminase, gamma-glutamyltransferase and alkaline phosphatase concentrations), only SHBG remained significantly associated with risk (OR for top versus bottom tertile of 3.86 (1.32-11.3), ptrend =0.009). As a single factor SHBG had an AUC of 0.81 (0.75-0.86). A small, but significant increase in AUC was observed when SHBG was added to a model including the liver damage score and epidemiological variables (from 0.89 to 0.91, p=0.02) and a net reclassification of 0.47% (0.45-0.48). The observed associations of HCC with pre-diagnostic SHBG, free testosterone and IGF-I concentrations are in directions opposite to that expected under the etiological hypotheses. SHBG has a potential to be tested as pre-diagnostic risk marker for HCC. © 2013 Wiley Periodicals, Inc.

Lukanova, Annekatrin; Becker, Susen

2014-01-01

346

Low-dose antithymocyte globulin enhanced the efficacy of tacrolimus and mycophenolate for GVHD prophylaxis in recipients of unrelated SCT.  

Science.gov (United States)

We performed a retrospective analysis of the outcome of 197 consecutive unrelated donor transplant recipients who received GVHD prophylaxis either TM regimen (tacrolimus and mycophenolate) (121 patients) or TM/ATG-G regimen (TM with low-dose antithymocyte globulin (ATG) of 4.5?mg/kg, ATG-G, Genzyme) (76 patients). Cumulative incidences of grade II-IV acute GVHD for the TM and TM/ATG-G cohorts were 49% and 61% (P=0.11) and grade III-IV acute GVHD for the TM and TM/ATG-G cohorts were 27% and 14% (P=0.02), respectively. There was no difference in the incidence of relapse or disease progression between TM and TM/ATG-G-16% and 23% (P=0.64). TM/ATG-G cohort had lower incidence of non-relapse mortality (NRM; 37% vs 20%, P=0.01), chronic GVHD (56% vs 43%, P<0.001) and more favorable global chronic GVHD severity (P<0.001). Univariate analyses showed improved OS and PFS of patients who received TM/ATG-G. Multivariate analysis confirmed TM/ATG-G had a favorable influence on OS (P=0.05) but not on PFS (P=0.07). We concluded that low-dose ATG of 4.5?mg/kg given in conjunction with TM improved GVHD prophylaxis without increased risk of relapse. Lower NRM, lower incidence and severity of chronic GVHD could potentially improve survival.Bone Marrow Transplantation advance online publication, 6 October 2014; doi:10.1038/bmt.2014.203. PMID:25285804

Ratanatharathorn, V; Deol, A; Ayash, L; Cronin, S; Bhutani, D; Lum, L G; Abidi, M; Ventimiglia, M; Mellert, K; Uberti, J P

2014-10-01

347

Characterization of the human sex hormone binding globulin (SHBG) gene and demonstration of two transcripts in both liver and testis  

Energy Technology Data Exchange (ETDEWEB)

A genomic cosmid clone for human sex hormone binding globulin (SHBG), a liver-secreted plasma glycoprotein that binds sex steroids, was isolated with a previously characterized liver cDNA as probe. Southern blot analysis of genomic DNA indicated that only one SHBG gene is present in the human haploid genome. A 3.8 Kb Xba I-fragment of the clone containing the entire coding region of SHBG was sequenced. The SHBG gene has 8 exons. The 5{prime}-end preceding the translation start site had no TATA box or CAAT box promoter elements. Screening of a human testis cDNA library resulted in the isolation of two distinct cDNA forms. One cDNA was identical with the previously characterized liver SHBG cDNA, thus suggesting that human SHBG and the androgen binding protein (ABP) produced by Sertoli cells are coded for by the same gene. The second cDNA differed from the first by having exon I exchanged with a completely different sequence and exon VII deleted. An exon coding for the 5{prime}-end of this cDNA was found in the cosmid clone 1.5 kb upstream of the first SHBG exon. Primer extension experiments showed the alternatively spliced transcript corresponding to the second cDNA to be present in both liver and testis. From the primary structure of this putative SHBG-gene-related protein, it may be deduced that it is a protein very different from SHBG and probably without steroid binding activity.

Gershagen, S.; Lundwall, A.; Fernlund, P. (Univ. of Lund, Malmoe (Sweden))

1989-11-25

348

Regulation of IgM anti-bovine. gamma. -globulin antibody formation by helper and suppressor T cells in rabbits  

Energy Technology Data Exchange (ETDEWEB)

Primary IgM anti-bovine ..gamma..-globulin (BGG) antibody formation was studied in rabbits subjected to 500 or 900 R of whole body X-irradiation in combination with one or more of the following conditions: appendectomy (Ax), thymectomy (Tx), shielding of appendix (As) or bone marrow (Bs) during irradiation (X), transfer of 10/sup 9/ appendix or thymus cells (Ac or Tc), and immediate immunization with 200 ..mu..g of alum-precipitated BGG iv. The IgM antibody response was well preserved in AsX rabbits in comparison with X/sub 500/ rabbits. Conversely, the response was suppressed in AxX/sub 500/ rabbits although transfer of autologous appendix cells to these animals (AxX/sub 500/Ac) restored the response. In the group receiving 900 R, there was variable restoration in individual AxX.Ac and AxBsX.Ac rabbits and little or none in AxTxX.Tc, AxTxBsX.Tc, and AxTxBsX.Ac animals. However, simultaneous injections of autologous thymus cells and appendix lymphocytes (AxTxX.Ac.Tc) restored the response completely. The IgM response was somewhat enhanced in AsTxX animals and returned again to the level of normal controls by injecting these animals with autologous thymus cells. However, marked suppression was seen when AsTxX rabbits were injected with neonatal allogeneic thymus cells. A remarkable enhancement of IgM antibody formation was induced in normal rabbits by injection of PHA into the appendiceal artery. On the basis of these results and of histological observations, it was suggested that the rabbit appendix represents a large pool of both B and T cells and that appendix-derived B cells participating in IgM anti-BGG antibody formation are under the regulation of both helper and suppressor T cells.

Hanaoka, M.; Mizumoto, T.; Takigawa, M.

1977-06-01

349

Long-term outcome of 25 children and adolescents with severe aplastic anemia treated with antithymocyte globulin  

Directory of Open Access Journals (Sweden)

Full Text Available Severe aplastic anemia (SAA is probably an immune-mediated disorder, and immunosuppressive therapy is recommended for patients with no available donor for bone marrow transplant. Between October 1984 and November 1987, 25 consecutive children and adolescents with SAA with no HLA-compatible marrow donor received equine antithymocyte globulin (ATG (15 mg kg-1 day-1 for 10 days. The patients were evaluated 6 weeks, 6 months, and 12 months after starting ATG treatment. Thereafter, patients were evaluated yearly until July 1998. Median age was 10 years (range, 1.5-20 years, granulocyte counts on referral ranged from 0.032 to 1.4 x 10(9/l (median 0.256 x 10(9/l, and 12 patients had granulocyte counts <0.2 x 10(9/l. At a median follow-up of 9.6 years (range, 8.6-11.8 years, 10 patients (40% remained alive with good marrow function. No morphologic evidence of hematological clonal disorders has been observed, although two patients probably have acquired clonal chromosomal abnormalities (trisomy 8 and del(6q21, respectively. Responses to ATG were observed between 6 weeks and 6 months from the start of treatment in 60% of evaluable patients. The response rate was not different in patients whose granulocyte count at diagnosis was <0.2 x 10(9/l, or in those who were <10 years of age. This study supports the view that, when compared with supportive measures, ATG is an effective treatment for children or adolescents with SAA. Although these results are inferior to those reported for marrow transplantation or more intensive immunosuppressive regimens, these patients who responded to ATG are long-term survivors with stable peripheral blood counts and a low rate of relapse.

de-Medeiros C.R.

2000-01-01

350

A retrospective comparison of cyclophosphamide plus antithymocyte globulin with cyclophosphamide plus busulfan as the conditioning regimen for severe aplastic anemia  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Allogeneic hematopoietic stem cell transplantation (AHSCT) is the treatment of choice for young patients with severe aplastic anemia (SAA). The association of antithymocyte globulin (ATG) and cyclophosphamide (CY) is the most frequently used conditioning regimen for this disease. We performed this r [...] etrospective study in order to compare the outcomes of HLA-matched sibling donor AHSCT in 41 patients with SAA receiving cyclophosphamide plus ATG (ATG-CY, N = 17) or cyclophosphamide plus busulfan (BU-CY, N = 24). The substitution of BU for ATG was motivated by the high cost of ATG. There were no differences in the clinical features between the two groups, including age, gender, cytomegalovirus status, ABO match, interval between diagnosis and transplant, and number of total nucleated cells infused. No differences were observed in the time to neutrophil and platelet engraftment, or in the risk of veno-occlusive disease and hemorrhage. However, there was a higher risk of mucositis in the BU-CY group (71 vs 24%, P = 0.004). There were no differences in the incidence of neutrophil and platelet engraftment, acute and chronic graft-versus-host disease, and transplant-related mortality. There was a higher incidence of late rejection in the ATG-CY group (41 vs 4%, P = 0.009). Although the ATG-CY group had a longer follow-up (101 months) than the BU-CY group (67 months, P = 0.04), overall survival was similar between the groups (69 vs 58%, respectively, P = 0.32). We conclude that the association BU-CY is a feasible option to the conventional ATG-CY regimen in this population.

L.V.M., Ommati; C.A., Rodrigues; A.R., Silva; L.P., Silva; M.L.L.F., Chaufaille; J.S.R., Oliveira.

2009-03-01

351

A retrospective comparison of cyclophosphamide plus antithymocyte globulin with cyclophosphamide plus busulfan as the conditioning regimen for severe aplastic anemia  

Directory of Open Access Journals (Sweden)

Full Text Available Allogeneic hematopoietic stem cell transplantation (AHSCT is the treatment of choice for young patients with severe aplastic anemia (SAA. The association of antithymocyte globulin (ATG and cyclophosphamide (CY is the most frequently used conditioning regimen for this disease. We performed this retrospective study in order to compare the outcomes of HLA-matched sibling donor AHSCT in 41 patients with SAA receiving cyclophosphamide plus ATG (ATG-CY, N = 17 or cyclophosphamide plus busulfan (BU-CY, N = 24. The substitution of BU for ATG was motivated by the high cost of ATG. There were no differences in the clinical features between the two groups, including age, gender, cytomegalovirus status, ABO match, interval between diagnosis and transplant, and number of total nucleated cells infused. No differences were observed in the time to neutrophil and platelet engraftment, or in the risk of veno-occlusive disease and hemorrhage. However, there was a higher risk of mucositis in the BU-CY group (71 vs 24%, P = 0.004. There were no differences in the incidence of neutrophil and platelet engraftment, acute and chronic graft-versus-host disease, and transplant-related mortality. There was a higher incidence of late rejection in the ATG-CY group (41 vs 4%, P = 0.009. Although the ATG-CY group had a longer follow-up (101 months than the BU-CY group (67 months, P = 0.04, overall survival was similar between the groups (69 vs 58%, respectively, P = 0.32. We conclude that the association BU-CY is a feasible option to the conventional ATG-CY regimen in this population.

L.V.M. Ommati

2009-03-01

352

PACS storage technology update: holographic storage.  

Science.gov (United States)

This paper focuses on the emerging technology of holographic storage and its effect on picture archiving and communication systems (PACS). A review of the emerging technology is presented, which includes a high level description of holographic drives and the associated substrate media, the laser and optical technology, and the spatial light modulator. The potential advantages and disadvantages of holographic drive and storage technology are evaluated. PACS administrators face myriad complex and expensive storage solutions and selecting an appropriate system is time-consuming and costly. Storage technology may become obsolete quickly because of the exponential nature of the advances in digital storage media. Holographic storage may turn out to be a low cost, high speed, high volume storage solution of the future; however, data is inconclusive at this early stage of the technology lifecycle. Despite the current lack of quantitative data to support the hypothesis that holographic technology will have a significant effect on PACS and standards of practice, it seems likely from the current information that holographic technology will generate significant efficiencies. This paper assumes the reader has a fundamental understanding of PACS technology. PMID:16783957

Colang, John E; Johnston, James N

2006-01-01

353

Wind turbine storage systems  

International Nuclear Information System (INIS)

Electric power is often produced in locations far from the point of utilization which creates a challenge in stabilizing power grids, particularly since electricity cannot be stored. The production of decentralized electricity by renewable energy sources offers a greater security of supply while protecting the environment. Wind power holds the greatest promise in terms of environmental protection, competitiveness and possible applications. It is known that wind energy production is not always in phase with power needs because of the uncertainty of wind. For that reason, energy storage is the key for the widespread integration of wind energy into the power grids. This paper proposed various energy storage methods that can be used in combination with decentralized wind energy production where an imbalance exists between electricity production and consumption. Energy storage can play an essential role in bringing value to wind energy, particularly if electricity is to be delivered during peak hours. Various types of energy storage are already in use or are being developed. This paper identified the main characteristics of various electricity storage techniques and their applications. They include stationary or embarked storage for long or short term applications. A comparison of characteristics made it possible to determine which types of electricity storage are best suited for wind energy. These include gravity energy; thermal energy; compressed air energy; coupled stornergy; compressed air energy; coupled storage with natural gas; coupled storage with liquefied gas; hydrogen storage for fuel cells; chemical energy storage; storage in REDOX batteries; storage by superconductive inductance; storage in supercondensers; and, storage as kinetic energy. 21 refs., 21 figs

354

Níveis séricos de globulinas e a intensidade da fibrose hepática em pacientes com esquistossomose mansônica / Serum globulin levels and intensity of hepatic fibrosis in patients with mansonic schistosomiasis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese CONTEXTO: Tem sido descrita correlação entre os níveis séricos de globulinas e o grau de fibrose hepática nas hepatites crônicas, mas não se encontram relatos na esquistossomose mansônica. OBJETIVO: Avaliar os níveis séricos de globulinas e de IgG, e a intensidade da fibrose periportal mensurada pel [...] a ultrassonografia em pacientes com esquistossomose mansônica. MÉTODOS: Entre novembro de 2006 e fevereiro de 2007, foram estudados 41 pacientes que preencheram ficha clínica e realizaram dosagens de IgG por imunoturbidimetria e de globulinas indiretamente pelo método do biureto. A ultrassonografia foi realizada por um único pesquisador, seguindo os protocolos do Cairo e de Niamey. RESULTADOS: A média de idade foi 41 anos, sendo 25 pacientes (61%) do sexo feminino. Dez dos 41 pacientes (24%) apresentaram elevação dos níveis séricos de globulinas e 21 (51%) dos de IgG. Conforme a classificação do Cairo, 21 pacientes apresentaram grau I de fibrose, 18 grau II e 2 grau III, e pela classificação de Niamey 8 apresentavam padrão C, 20 D e 13 E. Aqueles com graus II ou III de fibrose tiveram maiores níveis de IgG do que os de grau I (P = 0,047), assim como aqueles que apresentaram padrões D e E em relação ao C (P = 0,011). Não houve associação entre os níveis de globulinas e o grau ou padrão de fibrose. CONCLUSÃO: Em pacientes com esquistossomose mansônica, observou-se elevação dos níveis séricos de IgG de acordo com a progressão do grau e do padrão de fibrose periportal, mas o mesmo não se observou com os níveis de globulinas. Abstract in english BACKGROUND: A correlation between the levels of serum globulins and the hepatic fibrosis degree in chronic hepatitis was described, but reports in schistosomiasis mansoni have not been found. OBJECTIVE: To evaluate the serum globulins and IgG levels, and periportal fibrosis intensity measured by ult [...] rasound in patients with schistosomiasis mansoni. METHODS: Between November, 2006 and February 2007, 41 patients which were eligible, filled them a questionnaire and had their levels of serum IgG measured by immunoturbidimetry and globulins indirectly measured by the Biuret method. The ultrasound was carried out by a single researcher, according to the Cairo and Niamey protocols. RESULTS: The average age was 41 years old and 25 female patients (61%). Ten patients (24%) from 41 showed serum globulins levels raised and 21 (51%) presented elevated IgG levels. According to the Cairo classification, 21 patients showed grade I of fibrosis, 18 grade II and 2 grade III; and by the Niamey classification 8 showed standard C, 20 D, and 13 E. Those with grade II or III of fibrosis had higher IgG levels than the ones with grade I (P = 0,047), as well as those who showed standards D and E as compared to C (P = 0,011). There was no association between the globulins levels and the intensity of fibrosis. CONCLUSION: In patients with schistosomiasis mansoni, an increase of the IgG serum levels was observed according to the progression from periportal fibrosis intensity, but the same was not founded with globulins levels.

Henrique S. T., Correia; Ana Lucia C., Domingues; Edmundo P. A., Lopes; Clarice N. L., Morais; Camila, Sarteschi; Izolda M. F., Moura.

2009-09-01

355

Cloud storage for dummies  

CERN Document Server

Understand cloud computing and save your organization time and money! Cloud computing is taking IT by storm, but what is it and what are the benefits to your organization? Hitachi Data Systems' Cloud Storage For Dummies provides all the answers, With this book, you discover a clear explanation of cloud storage, and tips for how to choose the right type of cloud storage for your organization's needs. You also find out how cloud storage can free up valuable IT resources, saving time and money. Cloud Storage For Dummies presents useful information on setting up a

Xu, Linda; Loughlin, Tanya

2010-01-01

356

14C-labeled pulegone and metabolites binding to alpha2u-globulin in kidneys of male F-344 rats.  

Science.gov (United States)

Pulegone is a major constituent of pennyroyal oil and a minor component of peppermint oil. Pulegone is biotransformed to menthofuran and menthones (diastereomeric menthone and isomenthone) in pennyroyal and peppermint as well as in rodents. Pulegone and menthofuran are hepatotoxic to rodents, and menthones are less toxic. The metabolism and disposition of pulegone and menthofuran were previously studied in rodents, and higher concentrations of pulegone- and menthofuran-derived radioactivity were observed in male than female rat kidney. One explanation is the association of pulegone and metabolites with a male rat-specific protein, alpha2u-globulin. To test this hypothesis, male and female rats were dosed orally with 14C-labeled pulegone (80 mg/kg, 120 microCi/kg) or menthofuran (60 mg/kg, 120 microCi/kg) or menthones (80 mg/kg, 120 microCi/kg) in corn oil, and the kidney cytosol was prepared 24 h after dosing. An equilibrium dialysis experiment showed that in all three studies the radioactivity was associated with kidney cytosol proteins of male but not female rats. The chemicals present in the male rat kidney cytosol after dialysis were extracted with dichloromethane and characterized by high-performance liquid chromatography (HPLC) and gas chromatography/mass spectrometry (GC-MS). All parent compounds were detected, and the metabolites characterized included piperitone from pulegone or menthones treatment, menthones and possibly 8-hydroxymenthones from pulegone treatment, and mintlactones (diastereomeric mintlactone and isomintlactone) and 7a-hydroxymintlactone from menthofuran treatment. Analysis of the male rat kidney cytosol by a gel filtration column demonstrated that the retention was due to reversible binding of these chemicals with the male rat-specific protein alpha2u-globulin. However, binding of pulegone and/or metabolites to alpha2u-globulin did not produce accumulation of this protein in the kidney. PMID:17687727

Ferguson, Ling-Jen Chen; Lebetkin, Edward H; Lih, Fred B; Tomer, Kenneth B; Parkinson, Horace D; Borghoff, Susan J; Burka, Leo T

2007-09-01

357

Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance  

DEFF Research Database (Denmark)

Background: It is not known why some foods sensitizing via the gastrointestinal tract are prevalent allergenic foods and others are not. Eating habits, processing, and the food matrix have been suggested to influence the allergenicity of a given food. Factors related to protein structure, such as stability to digestion, have also been suggested. 7S globulins from peanut, hazelnut, soy, and pea were studied to determine whether related proteins would induce a similar sensitization when removed from their ‘normal’ matrix. Methods: Brown Norway rats (soy tolerant or nontolerant) were immunized i.p. 3 times with 100 ?g purified peanut, hazelnut, soy, or pea 7S without adjuvant. Sera were analyzed for specific antibodies by different ELISAs (IgG1, IgG2a, and IgE), inhibition ELISA, and rat basophilic leukemia cell assay. Results: The 4 related 7S globulins induced a response with an almost identical level of specific antibodies, but peanut 7S induced IgE of higher avidity than hazelnut and pea 7S which, again, hada higher avidity than IgE induced by soy 7S. Soy tolerance reduced the functionality of IgE without influencing antibody titers. Conclusions: Although the 4 7S globulins are structurally related allergens, they induce antibodies with different antigen-binding characteristics. Peanut 7S induces IgE of a higher avidity than hazelnut and pea 7S which, again, has a higher avidity than IgE induced by soy 7S. We also show that soy tolerance influences the function of antibodies to peanut 7S. These findings may help explain how antibodies of different clinical significances can develop in different individuals sensitized to the same allergen.Copyright © 2011 S. Karger AG, Basel

Kroghsbo, Stine; BØgh, Katrine Lindholm

2011-01-01

358

Spent fuel storage rack  

International Nuclear Information System (INIS)

Purpose: To improve the safety and facilitate the design by limiting the relative displacement in a storage rack. Constitution: The outer wall of a storage rack disposed in water within a fuel pool, the pool wall opposing to the storage rack and the structure between the opposing storages racks are made as a space for confining the pool water or a structure formed with a slight gap, for example, a combination of a recessed structure and a protruded structure. In such a constitution, a space for confirming the pool water is established and the pool water thus confined forms a flow resistance when the storage rack vibrates upon earthquakes, serves as a damper and significantly reduces the responsivity. Furthermore, the relative displacement in the storage rack is limited to inhibit excess earthquake forces to exert on setting bolts and rack clamping bolts of the storage rack. (Sekiya, K.)

359

Control of the membrane sex hormone-binding globulin-receptor (SHBG-R) in MCF-7 cells: effect of locally produced SHBG.  

OpenAIRE

The interaction between plasma sex hormone-binding globulin (SHBG) and its receptor (SHBG-R) inhibits estradiol-induced proliferation of MCF-7 cells (human estrogen-dependent breast cancer) through cAMP and PKA. Thus, SHBG can modulate estradiol action in breast cancer, but the implications of this require a more detailed knowledge of the SHBG-R. To this end, we have transfected MCF-7 cells with an expression vector carrying the human SHBG cDNA (S-MCF-7) and studied the effects of this on bot...

Frairia, Roberto

1998-01-01

360

Primary structure of human corticosteroid binding globulin, deduced from hepatic and pulmonary cDNAs, exhibits homology with serine protease inhibitors.  

OpenAIRE

We have isolated and sequenced cDNAs for corticosteroid binding globulin (CBG) prepared from human liver and lung mRNAs. Our results indicate that CBG mRNA is relatively abundant in the liver but is also present in the lung, testis, and kidney. The liver CBG cDNA contains an open reading frame for a 405-amino acid (Mr 45,149) polypeptide. This includes a predominantly hydrophobic, leader sequence of 22 residues that precedes the known NH2-terminal sequence of human CBG. We, therefore, predict...

Hammond, G. L.; Smith, C. L.; Goping, I. S.; Underhill, D. A.; Harley, M. J.; Reventos, J.; Musto, N. A.; Gunsalus, G. L.; Bardin, C. W.

1987-01-01

361

Sex hormone-binding globulin, its membrane receptor, and breast cancer: a new approach to the modulation of estradiol action in neoplastic cells.  

OpenAIRE

The role of human Sex Hormone-Binding Globulin (SHBG), the plasma carrier of sex steroids, and its membrane receptor, SHBG-R, in estrogen-dependent breast cancer has been investigated in our laboratory in the past few years. SHBG-R is expressed in MCF-10 A cells (not neoplastic mammary cells), MCF-7 cells (breast cancer, ER positive) and in tissue samples from patients affected with ER positive breast cancer, but not in estrogen-insensitive MDA-MB 231 cells. The SHBG/SHBG-R interaction, follo...

Frairia, Roberto; Berta, Laura Adelaide Angela

1999-01-01

362

A case of non-regenerative immune-mediated anemia treated by combination therapy of human immune globulin and mycophenolate mofetil in a dog  

Directory of Open Access Journals (Sweden)

Full Text Available A 12-year-old female Shih Tzu dog was referred with diarrhea. Hematological examination indicated severe non-regenerative anemia. Bone marrow aspiration smears and core biopsy specimens revealed normal bone marrow. Based on those results, non-regenerative immune-mediated anemia was diagnosed. The dog was initially treated using prednisolone and cyclosporine. However, this treatment regimen did not prove effective. Nevertheless, the patient achieved a good hematological response after the administration of a combination therapy of human immune globulin and mycophenolate mofetil. Such a combination therapy may prove effective against non-regenerative immune-mediated anemia.

M. Yuki

2011-12-01

363

Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin)  

OpenAIRE

Studies were carried out on the interactions of uncharged latex beads (0.76 micrometer) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However,...

1980-01-01

364

Energy storage technologies  

International Nuclear Information System (INIS)

This book takes stock of the advantages and drawbacks of the different energy storage solutions apart from the classical fossil fuels (oil, uranium, gas), and details the technologies developed for an electric end-use. Storage is one of the most critical point for the development of new energy technologies, in particular those that use the electricity vector all along the energy source chain (generation, production, transport, utilisation). Storage is important not only for individual or independent applications, that use renewable energies or not, often intermittent, but also to secure coupled systems like power transportation and distribution systems. The development and choice of the most relevant technologies is dependent of technical-economical parameters. It can also supply new services, in particular in the framework of new electricity markets. Content: power film-capacitors, magnetic storage, kinetic energy storage, compressed air energy storage (CAES), hydro-pneumatic storage, high-temperature thermal storage of electricity, hydraulic gravity storage, power electronic systems for energy storage. (J.S.)

365

Characteristics of chemical binding to alpha 2u-globulin in vitro--evaluating structure-activity relationships  

International Nuclear Information System (INIS)

alpha 2u-Globulin (alpha 2u) has been shown to accumulate in the kidneys of male rats treated with 2,2,4-trimethylpentane (TMP). 2,4,4-Trimethyl-2-pentanol (TMP-2-OH), a metabolite of TMP, is found reversibly bound to alpha 2u isolated from the kidneys of these treated rats. The objectives of the following study were to characterize the ability of [3H]TMP-2-OH to bind to alpha 2u in vitro and to determine whether other compounds that cause this protein to accumulate have the same binding characteristics. Although compounds that have been shown to cause the accumulation of alpha 2u in male rat kidneys compete in vitro with [3H]TMP-2-OH for binding to alpha 2u, they do so to varying degrees. The binding affinity (Kd) of the [3H]TMP-2-OH-alpha 2u complex was calculated to be on the order of 10(-7) M. The inhibition constant values (Ki) determined for d-limonene, 1,4-dichlorobenzene, and 2,5-dichlorophenol were all in the range 10(-4) M, whereas the Ki values for isophorone, 2,4,4- or 2,2,4-trimethyl-1-pentanol, and d-limonene oxide were determined to be in the range 10(-6) and 10(-7) M, respectively. TMP and 2,4,4- and 2,2,4-trimethylpentanoic acid did not compete for binding. This suggests that other factors, besides binding, are involved in the accumulation of alpha 2u. In this study the ability of a chemical to bind to alpha 2u was used as a measure of biological activity to assess structure-activity relationships among the chemicals tested and known to cause the accuemicals tested and known to cause the accumulation of alpha 2u. The results so far suggest that binding is dependent on both hydrophobic interactions and hydrogen bonding

366

Differential binding of thyroxine and triiodothyronine to acidic isoforms of thyroid hormone binding globulin in human serum  

International Nuclear Information System (INIS)

The differential availability of thyroxine (T4) and 3,5,3'-triiodothyronine (T3) to liver from the circulating thyroid hormone binding globulin (TBG)-bound pool suggests that the two thyroid hormones may bind to different TBG isoforms in human serum. In the present study, the binding of [125I]T4 and [125I]T3 to human serum proteins was investigated by using slab gel isoelectric focusing and chromatofocusing. In normal human male serum, [125I]T4 was localized to four isoforms of TBG called TBG-I, -II, -III, and -IV, with isoelectric points (pI's) of 4.30, 4.35, 4.45, and 4.55, respectively. [125I]T3 was localized to only two isoforms of TBG, TBG-III, and -IV, with pI's that were identical with those for [125I]T4. In normal female serum, [125I]T4 was localized to the same four isoforms of TBG as those of normal male serum, while [125I]T3 was localized to TBG-II, -III, -IV, and -V (pI = 4.65). In pregnant female serum, [125I]T4 was localized to five isoforms, whereas [125I]T3 was localized to four. IEF was also performed with male serum loaded with various concentrations of unlabeled T3. The K/sub i/ values of T3 binding to TBG-I, -II, -III, and -IV were 5.0, 2.4, 0.86, and 0.46 nM, respectively. The TBG isoforms in normal male serumely. The TBG isoforms in normal male serum were also separated by sequential concanavalin A-Sepharose affinity chromatography and the chromatofocusing (pH range of 3.5-5.0). T4 preferentially bound to the most acidic isoforms of TBG in the pI range of 3.8-4.0, whereas the less acidic fractions (pH 4.0-4.2) bound both T4 and T3. In conclusion, this study shows that T4 and T3 do not bind to a single competitive binding site on TBG. Instead, T4 is preferentially bound by the most acidic TBG isoforms owing to a 10-fold lower affinity of T3 for these proteins

367

Rationality analysis of delivery method for hepatitis B immune globulin in patients with chronic HBV infection after liver transplantation  

Directory of Open Access Journals (Sweden)

Full Text Available ObjectiveTo analyze the rationality of delivery method for hepatitis B immune globulin (HBIG in patients with chronic hepatitis B virus (HBV infection after liver transplantation and to investigate the guiding principle for medication. MethodsForty-four cases of hepatitis B-related liver transplantation who participated in the phase IV clinical trial of HBIG for intravenous injection from August 2008 to December 2010 were analyzed. These patients were divided into severe liver disease group, liver cancer group, and liver cirrhosis group. The positive rates of HBV DNA and HBeAg were compared between groups by chi-square test. The HBV DNA level was compared by analysis of variance. The correlation between blood concentration and half-life of HBIG at different doses and dosing intervals was analyzed by Pearson?s correlation test. The titer of anti-HBs at one week after operation was compared between HBeAg-negative group and HBeAg-positive group and between high-HBV DNA group and low-HBV DNA group by t test. Cases of reinfection were also monitored. Results There were no significant differences in the positive rates of HBV DNA and HBeAg between the severe liver disease group, liver cancer group, and liver cirrhosis group (?2=4.871, P=0.088; ?2=1.079, P=0.583. No significant differences in mean HBV DNA level were found between these groups (F=0.895, P=0.418. The Pearson?s correlation analysis revealed a significant negative correlation between the blood concentration and half-life of HBIG (r=0.988, P=0.012. The tier of anti-HBs showed no significant differences between HBeAg-negative group and HBeAg-positive group and between high-HBV DNA group and low-HBV DNA group one week after operation (t=1.757, P=0.087. No cases of reinfection were found during the observation period. ConclusionIt is necessary to give HBIG 4000 IU during operation and 2000 IU daily for 6 days after operation, and the dose should be increased appropriately when the patients have relatively high viral load in serum. For the patients whose serological markers of hepatitis B become negative, the antibody level can be maintained at about 100 IU/L until the next administration if 600 IU of HBIG is given once a month by intramuscular injection.

WEI Xianyi

2014-01-01

368

Identification, characterization and expression of novel Sex Hormone Binding Globulin alternative first exons in the human prostate  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background The human Sex Hormone Binding Globulin (SHBG gene, located at 17p13.1, comprises, at least, two different transcription units regulated by two different promoters. The first transcription unit begins with the exon 1 sequence and is responsible for the production of plasma SHBG by the hepatocytes, while the second begins with an alternative exon 1 sequence, which replaces the exon 1 present in liver transcripts. Alternative exon 1 transcription and translation has only been demonstrated in the testis of transgenic mice containing an 11-kb human SHBG transgene and in the human testis. Our goal has been to further characterize the 5' end of the SHBG gene and analyze the presence of the SHBG alternative transcripts in human prostate tissue and derived cell lines. Results Using a combination of in silico and in vitro studies, we have demonstrated that the SHBG gene, along with exon 1 and alternative exon 1 (renamed here exon 1A, contains four additional alternative first exons: the novel exons 1B, 1C, and 1E, and a previously identified exon 1N, which has been further characterized and renamed as exon 1D. We have shown that these four alternative first exons are all spliced to the same 3' splice site of SHBG exon 2, and that exon 1A and the novel exon 1B can be spliced to exon 1. We have also demonstrated the presence of SHBG transcripts beginning with exons 1B, 1C and 1D in prostate tissues and cell lines, as well as in several non-prostatic cell lines. Finally, the alignment of the SHBG mammalian sequences revealed that, while exons 1C, 1D and 1E are very well conserved phylogenetically through non-primate mammal species, exon 1B probably aroused in apes due to a single nucleotide change that generated a new 5' splice site in exon 1B. Conclusion The identification of multiple transcription start sites (TSS upstream of the annotated first exon of human SHBG, and the detection of the alternative transcripts in human prostate, concur with the prediction of the ENCODE (ENCyclopedia of DNA Elements project, and suggest that the regulation of SHBG is much more complex than previously reported.

de Torres Inés

2009-06-01

369

Comparison of cost of immune globulin intravenous therapy to conventional immunosuppressive therapy in treating patients with autoimmune mucocutaneous blistering diseases.  

Science.gov (United States)

Autoimmune mucocutaneous blistering diseases (AMBD) are a group of potentially fatal diseases that affect the skin and mucous membranes. AMBD have different target antigens as well as variable clinical presentation, course, and prognosis. The mainstay of conventional immunosuppressive therapy (CIST) for AMBD is long-term high-dose systemic corticosteroids and immunosuppressive agents. Such therapy has proven effective in many patients; however, in some patients, the disease continues to progress with significant sequelae such as blindness, loss of voice, anal, and vaginal stenosis which causes poor quality of life. Furthermore, the CIST may have some serious side effects including opportunistic infections which may cause death. Immune globulin intravenous (IGIV) therapy has been reportedly used in the management of patients with AMBD refractory to CIST. IGIV has shown to be more clinically beneficial than CIST by bringing about long-term clinical remission and less recurrence. The high cost of the IGIV is of concern to patients, physicians, and insurance companies. In this report, we compare the cost of IGIV to that of CIST in treating a cohort of 15 mucous membrane pemphigoid (MMP), 10 ocular cicatricial pemphigoid (OCP), 15 bullous pemphigoid (BP), and 32 pemphigus vulgaris (PV) patients. In each cohort of patients, CIST had significant side effects, many of which were hazardous and required prolonged and frequent hospitalizations. Some of these side effects were severe enough to require discontinuation of the treatment. We consider the total cost of CIST to be the actual cost of the drug, plus the cost of management of the side effects produced by CIST. In the same patient cohort, no significant side effects to IGIV were observed. None of the IGIV treated patients required physician visits, laboratory tests, or hospitalizations specifically related to IGIV therapy. Hence, the total cost of the IGIV therapy is the actual cost of the IGIV only. The mean total cost of treatment of IGIV therapy is statistically significantly less than that of CIST during the entire course of the disease and on an annual basis. In conclusion, IGIV therapy is a safe, clinically beneficial, and a cost effective alternative treatment in patients with AMBD, non-responsive to CIST. PMID:16504922

Daoud, Yassine J; Amin, Ketan G

2006-04-01

370

Differential binding of thyroxine and triiodothyronine to acidic isoforms of thyroid hormone binding globulin in human serum  

Energy Technology Data Exchange (ETDEWEB)

The differential availability of thyroxine (T/sub 4/) and 3,5,3'-triiodothyronine (T/sub 3/) to liver from the circulating thyroid hormone binding globulin (TBG)-bound pool suggests that the two thyroid hormones may bind to different TBG isoforms in human serum. In the present study, the binding of (/sup 125/I)T/sub 4/ and (/sup 125/I)T/sub 3/ to human serum proteins was investigated by using slab gel isoelectric focusing and chromatofocusing. In normal human male serum, (/sup 125/I)T/sub 4/ was localized to four isoforms of TBG called TBG-I, -II, -III, and -IV, with isoelectric points (pI's) of 4.30, 4.35, 4.45, and 4.55, respectively. (/sup 125/I)T/sub 3/ was localized to only two isoforms of TBG, TBG-III, and -IV, with pI's that were identical with those for (/sup 125/I)T/sub 4/. In normal female serum, (/sup 125/I)T/sub 4/ was localized to the same four isoforms of TBG as those of normal male serum, while (/sup 125/I)T/sub 3/ was localized to TBG-II, -III, -IV, and -V (pI = 4.65). In pregnant female serum, (/sup 125/I)T/sub 4/ was localized to five isoforms, whereas (/sup 125/I)T/sub 3/ was localized to four. IEF was also performed with male serum loaded with various concentrations of unlabeled T/sub 3/. The K/sub i/ values of T/sub 3/ binding to TBG-I, -II, -III, and -IV were 5.0, 2.4, 0.86, and 0.46 nM, respectively. The TBG isoforms in normal male serum were also separated by sequential concanavalin A-Sepharose affinity chromatography and the chromatofocusing (pH range of 3.5-5.0). T/sub 4/ preferentially bound to the most acidic isoforms of TBG in the pI range of 3.8-4.0, whereas the less acidic fractions (pH 4.0-4.2) bound both T/sub 4/ and T/sub 3/. In conclusion, this study shows that T/sub 4/ and T/sub 3/ do not bind to a single competitive binding site on TBG. Instead, T/sub 4/ is preferentially bound by the most acidic TBG isoforms owing to a 10-fold lower affinity of T/sub 3/ for these proteins.

Terasaki, T.; Pardridge, W.M.

1988-05-17

371

High serum level of antithymocyte globulin immediately before graft infusion is associated with a low likelihood of chronic, but not acute, graft-versus-host disease.  

Science.gov (United States)

Rabbit antithymocyte globulin (ATG) is administered during transplant conditioning to decrease the risk of both acute graft-versus-host disease (aGVHD) and chronic graft-versus-host disease (cGVHD). Here we evaluated the relationship between the serum concentration of ATG (capable of binding to lymphocytes) immediately before graft infusion (day 0) or on day +7 or +28 post-transplantation and the development of aGVHD or cGVHD. We studied 180 patients whose conditioning included 4.5 mg/kg antithymocyte globulin (ATG; Thymoglobulin). For aGVHD, we found no association with ATG levels on day 0. Nevertheless, high day +7 and +28 ATG levels were associated with a low likelihood of aGVHD. For cGVHD, high ATG levels at all 3 time points (days 0, +7, and +28) were associated with a low likelihood of cGVHD. In conclusion, high-dose ATG administration at the time of graft infusion appears to inhibit the development of cGVHD, but not aGVHD; however, higher ATG levels on days +7 and +28 are associated with lower rates of both aGVHD and cGVHD. PMID:24727330

Chawla, Sumit; Dharmani-Khan, Poonam; Liu, Yiping; Prokopishyn, Nicole; Amlish Munir, Muhammad; Griffiths, Cameron; Khan, Faisal M; Stewart, Douglas A; Russell, James A; Daly, Andrew; Storek, Jan

2014-08-01

372

Large mass storage facility  

International Nuclear Information System (INIS)

The report of a committee to study the questions surrounding possible acquisition of a large mass-storage device is presented. The current computing environment at BNL and justification for an online large mass storage device are briefly discussed. Possible devices to meet the requirements of large mass storage are surveyed, including future devices. The future computing needs of BNL are prognosticated. 2 figures, 4 tables

373

Wet storage of roundwood  

OpenAIRE

Wet storage (sprinkling) of wood offers valuable protection against drying out and biological attack, but at the same time there are disadvantages to be considered. Negative effects in the forms of reduction in wood quality and the environmental impact of log yard run-off must be minimised in order to make wet storage effective. In the work underlying this thesis, the effect of different sprinkling water qualities on changes in wood properties during storage was studied. A new method for trea...

Jonsson, Maria

2004-01-01

374

Underground thermal energy storage  

CERN Document Server

""Underground Thermal Energy Storage"" (UTES) provide us with a flexible tool to combat global warming through conserving energy while utilizing natural renewable energy resources. Primarily, they act as a buffer to balance fluctuations in supply and demand of low temperature thermal energy. ""Underground Thermal Energy Storage"" provides an comprehensive introduction to the extensively-used energy storage method. ""Underground Thermal Energy Storage"" gives a general overview of UTES from basic concepts and classifications to operation regimes. As well as discussing general procedures for des

Lee, Kun Sang

2012-01-01

375

Hemochromatosis: Iron Storage Disease  

Science.gov (United States)

... Submit What's this? Submit Button CDC Features Hemochromatosis: Iron Storage Disease Language: English Español (Spanish) Share Compartir Excessive iron buildup in the body's tissues and organs can ...

376

HEATS: Thermal Energy Storage  

Energy Technology Data Exchange (ETDEWEB)

HEATS Project: The 15 projects that make up ARPA-E’s HEATS program, short for “High Energy Advanced Thermal Storage,” seek to develop revolutionary, cost-effective ways to store thermal energy. HEATS focuses on 3 specific areas: 1) developing high-temperature solar thermal energy storage capable of cost-effectively delivering electricity around the clock and thermal energy storage for nuclear power plants capable of cost-effectively meeting peak demand, 2) creating synthetic fuel efficiently from sunlight by converting sunlight into heat, and 3) using thermal energy storage to improve the driving range of electric vehicles (EVs) and also enable thermal management of internal combustion engine vehicles.

None

2012-01-01

377

Immune Globulin Intravenous Injection  

Science.gov (United States)

... of IGIV at a time. Depending on the product you receive, you may be told to store it in the refrigerator. If you store IGIV in the refrigerator, take your next dose from the refrigerator 1 hour before using it; place it in a clean, dry area to allow it to warm to room temperature. ...

378

Reversible storage of hydrogen  

International Nuclear Information System (INIS)

In the framework of the reversible hydrogen storage, this laboratory is studying the hydrogen storage and more specifically reversible metallic hydrides. As a light, high mass capacity and economical element, the magnesium seems to be a good solution. The authors presents the operating of this fuel cell and the first results. (A.L.B.)

379

Colossal Storage Corporation  

Science.gov (United States)

Colossal Storage is a company specializing in a new method of holographic data storage. While current technologies are hindered by area density, the proposed technology will expand into three dimensions and use the disk's volume to write data. Visitors will find some interesting insights into the technology and several white papers are available.

380

Final storage without reprocessing  

International Nuclear Information System (INIS)

After a wet storage of about 40 years the fuel bars are placed in a Cu-rack which is put into a Cu-container, the gaps are filled with Pb, the casing is done in bentonite, the ultimate storage bunkers are 500 m underground in granite or gneiss with a low ground water permeability. (HP)

381

Compact nuclear fuel storage  

Energy Technology Data Exchange (ETDEWEB)

Different constructions of racks for compact storage of spent fuel assemblies (FA) in ''cooling'' pools (CP) of NPPs with the BWR and PWR type reactors are described. Problems concerning nuclear and radiation safety and provision of necessary thermal conditions arising in such rack design are discussed. It is concluded that the problem of prolonged fuel storage at NPPs became very actual for many countries because of retardation of the rates of fuel reprocessing centers building. Application of compact storage racks is a promising solution of the problem of intermediate FA storage at NPPs. Such racks of stainless boron steel and with neutron absorbers in the form of boron carbide panels enable to increase the capacity of the present CP 2-2.6 times, and the period of FA storage in them up to 5-10 years.

Kiselev, V.V.; Churakov, Yu.A.; Danchenko, Yu.V.; Bylkin, B.K.; Tsvetkov, S.V.

1983-01-01

382

Compact nuclear fuel storage  

International Nuclear Information System (INIS)

Different constructions of racks for compact storage of spent fuel assemblies (FA) in ''coolin''g pools (CP) of NPPs with the BWR and PWR type reactors are described. Problems concerning nuclear and radiation safety and provision of necessary thermal conditions arising in such rack design are discussed. It is concluded that the problem of prolonged fuel storage at NPPs became Very actual for many countries because of retapdation of the rates of fuel reprocessing centers building. Application of compact storage racks is a promising solution of the problem of intermediate FA storage at NPPs. Such racks of stainless boron steel and with neutron absorbers in the from of boron carbide panels enable to increase the capacity of the present CP 2-2.6 times, and the period of FA storage in them up to 5-10 years

383

Plutonium storage criteria  

Energy Technology Data Exchange (ETDEWEB)

The Department of Energy has issued a technical standard for long-term (>50 years) storage and will soon issue a criteria document for interim (<20 years) storage of plutonium materials. The long-term technical standard, {open_quotes}Criteria for Safe Storage of Plutonium Metals and Oxides,{close_quotes} addresses the requirements for storing metals and oxides with greater than 50 wt % plutonium. It calls for a standardized package that meets both off-site transportation requirements, as well as remote handling requirements from future storage facilities. The interim criteria document, {open_quotes}Criteria for Interim Safe Storage of Plutonium-Bearing Solid Materials{close_quotes}, addresses requirements for storing materials with less than 50 wt% plutonium. The interim criteria document assumes the materials will be stored on existing sites, and existing facilities and equipment will be used for repackaging to improve the margin of safety.

Chung, D. [Scientech, Inc., Germantown, MD (United States); Ascanio, X. [Dept. of Energy, Germantown, MD (United States)

1996-05-01

384

Spent fuel storage  

International Nuclear Information System (INIS)

A space is formed underground by partitioning walls made of radiation shielding material, in which only a storage cell is contained. The storage cell comprises metal blocks made of a heat conductive material such as stainless steels. A great number of holes each having a predetermined diameter are vertically arranged to the metal block, and storage pits are constituted by the holes. Spent nuclear fuels are contained in a first columnar vessel and the first columnar vessel is further contained in a large second columnar vessel. Both of the first columnar vessel and the second columnar vessel are made of a durable metal material such as stainless steels. The second columnar vessel is inserted to the storage pit of the storage cell. With such a constitution, heat emitted from the second columnar vessel is transferred to the metal block by radiation and dissipated from the metal block. Accordingly, a heat dissipation efficiency can be increased. (I.N.)

385

Spent fuel storage racks  

International Nuclear Information System (INIS)

Purpose: To decrease a spent fuel storage area occupied in a storage pool by increasing the storage density of the spent fuel, thereby enabling semi-permanent storage. Constitution: A framework of a spent fuel storage racks is made of stainless steel; and cells for holding fuel rod assemblies are formed by fitting, in its wall surface, ceramic tiles produced by sintering a mixture of such medium materials as Al2O3, SiO2, and ZrO2 and such elements as Eu, Hf, Gd, Sm, Co and Rh used as neutron absorbers which emit gamma rays in the process of reaction to neutrons. The most desirable neutron absorber to be used is Hf which has a high neutron absorbing capacity and a long half-life, is capable of keeping on absorbing neutrons even after neutron absorption, and makes (n,?) neutron absorption reaction, emitting gamma rays without accompanying any change of the ceramic tiles with time. (Sekiya, K.)

386

Electricity Storage. Technology Brief  

Energy Technology Data Exchange (ETDEWEB)

Electricity storage is a key technology for electricity systems with a high share of renewables as it allows electricity to be generated when renewable sources (i.e. wind, sunlight) are available and to be consumed on demand. It is expected that the increasing price of fossil fuels and peak-load electricity and the growing share of renewables will result in electricity storage to grow rapidly and become more cost effective. However, electricity storage is technically challenging because electricity can only be stored after conversion into other forms of energy, and this involves expensive equipment and energy losses. At present, the only commercial storage option is pumped hydro power where surplus electricity (e.g. electricity produced overnight by base-load coal or nuclear power) is used to pump water from a lower to an upper reservoir. The stored energy is then used to produce hydropower during daily high-demand periods. Pumped hydro plants are large-scale storage systems with a typical efficiency between 70% and 80%, which means that a quarter of the energy is lost in the process. Other storage technologies with different characteristics (i.e. storage process and capacity, conversion back to electricity and response to power demand, energy losses and costs) are currently in demonstration or pre-commercial stages and discussed in this brief report: Compressed air energy storage (CAES) systems, Flywheels; Electrical batteries; Supercapacitors; Superconducting magnetic storage; and Thermal energy storage. No single electricity storage technology scores high in all dimensions. The technology of choice often depends on the size of the system, the specific service, the electricity sources and the marginal cost of peak electricity. Pumped hydro currently accounts for 95% of the global storage capacity and still offers a considerable expansion potential but does not suit residential or small-size applications. CAES expansion is limited due to the lack of suitable natural storage sites. Electrical batteries have a large potential with a number of new materials and technologies under development to improve performance and reduce costs. Heat storage is practical in CSP plants. The choice between large-scale storage facilities and small-scale distributed storage depends on the geography and demography of the country, the existing grid and the type and scale of renewable technologies entering the market. While the energy storage market is quickly evolving and expected to increase 20-fold between 2010 and 2020, many electricity storage technologies are under development and need policy support for further commercial deployment. Electricity storage considerations should be an integral part of any plans for electric grid expansion or transformation of the electricity system. Storage also offers key synergies with grid interconnection and methods to smooth the variability of electricity demand (demand side management)

Simbolotti, G. [Italian National Agency for New Technologies, Energy and Sustainable Economic Development ENEA, Rome (Italy); Kempener, R. [International Renewable Energy Agency IRENA, Bonn (Germany)

2012-04-15

387

Electricity storage using a thermal storage scheme  

Science.gov (United States)

The increasing use of renewable energy technologies for electricity generation, many of which have an unpredictably intermittent nature, will inevitably lead to a greater demand for large-scale electricity storage schemes. For example, the expanding fraction of electricity produced by wind turbines will require either backup or storage capacity to cover extended periods of wind lull. This paper describes a recently proposed storage scheme, referred to here as Pumped Thermal Storage (PTS), and which is based on "sensible heat" storage in large thermal reservoirs. During the charging phase, the system effectively operates as a high temperature-ratio heat pump, extracting heat from a cold reservoir and delivering heat to a hot one. In the discharge phase the processes are reversed and it operates as a heat engine. The round-trip efficiency is limited only by process irreversibilities (as opposed to Second Law limitations on the coefficient of performance and the thermal efficiency of the heat pump and heat engine respectively). PTS is currently being developed in both France and England. In both cases, the schemes operate on the Joule-Brayton (gas turbine) cycle, using argon as the working fluid. However, the French scheme proposes the use of turbomachinery for compression and expansion, whereas for that being developed in England reciprocating devices are proposed. The current paper focuses on the impact of the various process irreversibilities on the thermodynamic round-trip efficiency of the scheme. Consideration is given to compression and expansion losses and pressure losses (in pipe-work, valves and thermal reservoirs); heat transfer related irreversibility in the thermal reservoirs is discussed but not included in the analysis. Results are presented demonstrating how the various loss parameters and operating conditions influence the overall performance.

White, Alexander

2015-01-01

388

Bone marrow transplantation in thalassemia major patients using "short" anti-thymocyte globulin therapy in Shiraz, Southern Iran.  

Science.gov (United States)

Allogeneic bone marrow transplantation (BMT) was performed on 113 Iranian transfusion-dependent thalassemia major patients from May 1993 through September 2003. To have at least 2 years follow-up, we report BMT on 90 patients transplanted up to December 2001. The donors were human leukocyte antigen (HLA)-identical, mixed lymphocyte culture (MLC)-nonreactive siblings (n = 74) on parents (n = 6); HLA-identical MLC-reactive siblings (n = 5) or parents (n = 1); and one HLA antigen-mismatched sibling (n = 4). The induction regimen in 11 patients was oral busulfan (BU) (14 mg/kg) and IV cyclophosphamide (CY; 200 mg/kg); in fifteen patients it was BU (15 mg/kg) and cyclophosphamide (CY; 200 mg/kg); in 47 patients, BU (15 mg/kg), CY (200 mg/kg), and short course of anti-thymocyte globulin (ATG, horse; 40 mg/kg including 10 mg/kg on days -2, -1, +1, +2); and in 15 patients, BU (15 mg/kg) CY (200 mg/kg), and ATG (60 to 100 mg/kg; 10 mg/kg at 3 to 5 days before and after BMT). Graft-versus-host disease (GVHD) prophylaxis consisted of cyclosporine and prednisolone. The group who received BU (14 mg/kg) and CY (200 mg/kg), as compared to the group receiving BU (15 mg/kg) and CY (200 mg/kg), was of younger age and lower risk; median age 7 versus 10 years, and 46% versus 7% in Lucarelli's risk group class I (the best prognostic group), respectively. These patients showed a lower disease-free survival (DFS), namely 64% versus 73%, with a follow up of 2 to 10.5 years. Thus from 9.5 years ago, our standard protocol for BU has been 15 mg/kg. The group who received "short" ATG (40 mg/kg), BU (15 mg/kg), and CY (200 mg/kg) showed almost the same outcome as the group who received a higher dose of ATG (60 to 100 mg/kg), namely DFS 72% versus 73%, respectively, despite the fact that half of both groups were included in the Lucarelli's risk group class III (the worst prognostic group) 49% versus 53%. We showed the same DFS for the patients who received BU (15 mg/kg), CY (200 mg/kg), and no ATG compared with the ATG group (73% vs 72%), but 27% of the group without ATG developed grade IV acute GVHD and 54% developed chronic GVHD. In the group with short ATG, 15% and 17% of patients developed grade IV acute and chronic GVHD, respectively. There was no significant difference for falls in platelets and white blood cell or engraftment days and the number of packed red blood cell transfusions among the groups. The median hospital stay was longer for the group with BU (15 mg/kg), CY (200 mg/kg) namely 81 versus 61 to 65 days. Second bone marrow infusions were needed in 6% and 20% of patients who received ATG doses of (40 versus 60 to 100 mg/kg; respectively (1 to 2 month post-BMT). BU at a dose of 15 mg/kg was more effective than 14 mg/kg BU for its myeloablative properties. By adding "short" ATG course to the conditioning regimen, the incidence of grade IV acute and chronic GVHD was reduced in thalassemic patients, especially when an HLA disparity was present. PMID:16387149

Zakerinia, M; Khojasteh, H N; Ramzi, M; Haghshenas, M

2005-12-01

389

SERI solar energy storage program  

Science.gov (United States)

Thermal energy storage technologies are identified for specific solar thermal applications. The capabilities and limitations of direct-contact thermal storage and thermochemical energy storage and transport are examined. Storage of energy from active solar thermal systems for industrial process heat and the heating of buildings is analyzed and seasonal energy storage is covered. The coordination of numerous thermal energy storage research and development activities is described.

Baylin, F.; Copeland, R. J.; Kotch, A.; Kriz, T.; Luft, W.; Nix, R. G.; Wright, J. O.

1982-05-01

390

Aflatoxins & Safe Storage  

Directory of Open Access Journals (Sweden)

Full Text Available The paper examines both field experience and research on the prevention of the exponential growth of aflatoxins during multi-month post harvest storage in hot, humid countries. The approach described is the application of modern safe storage methods using flexible, Ultra Hermetic™ structures that create an unbreatheable atmosphere through insect and microorganism respiration alone, without use of chemicals, fumigants, or pumps. Laboratory and field data are cited and specific examples are given describing the uses of Ultra Hermetic storage to prevent the growth of aflatoxins with their significant public health consequences. Also discussed is the presently limited quantitative information on the relative occurrence of excessive levels of aflatoxin (>20 ppb before versus after multi-month storage of such crops as maize, rice and peanuts when under high humidity, high temperature conditions and, consequently, the need for further research to determine the frequency at which excessive aflatoxin levels are reached in the field versus after months of post-harvest storage. The significant work being done to reduce aflatoxin levels in the field is mentioned, as well as its probable implications on post harvest storage. Also described is why, with some crops such as peanuts, using Ultra Hermetic storage may require injection of carbon dioxide or use of an oxygen absorber as an accelerant. The case of peanuts is discussed and experimental data is described.

PhilippeVillers

2014-04-01

391

SERI Solar Energy Storage Program  

Energy Technology Data Exchange (ETDEWEB)

The SERI Solar Energy Storage Program provides research on advanced technologies, system analyses, and assessments of thermal energy storage for solar applications in support of the Thermal and Chemical Energy Storage Program of the DOE Division of Energy Storage Systems. Currently, research is in progress on direct contact latent heat storage and thermochemical energy storage and transport. Systems analyses are being performed of thermal energy storage for solar thermal applications, and surveys and assessments are being prepared of thermal energy storage in solar applications.

Copeland, R. J.; Wright, J. D.; Wyman, C. E.

1980-02-01

392

Lipid storage myopathy.  

Science.gov (United States)

Lipid storage myopathy (LSM) is pathologically characterized by prominent lipid accumulation in muscle fibers due to lipid dysmetabolism. Although extensive molecular studies have been performed, there are only four types of genetically diagnosable LSMs: primary carnitine deficiency (PCD), multiple acyl-coenzyme A dehydrogenase deficiency (MADD), neutral lipid storage disease with ichthyosis, and neutral lipid storage disease with myopathy. Making an accurate diagnosis, by specific laboratory tests including genetic analyses, is important for LSM as some of the patients are treatable: individuals with PCD show dramatic improvement with high-dose oral L-carnitine supplementation and increasing evidence indicates that MADD due to ETFDH mutations is riboflavin responsive. PMID:21046290

Liang, Wen-Chen; Nishino, Ichizo

2011-02-01

393

Memory mass storage  

Energy Technology Data Exchange (ETDEWEB)

Memory Mass Storage describes the fundamental storage technologies, like Semiconductor, Magnetic, Optical and Uncommon, detailing the main technical characteristics of the storage devices. It deals not only with semiconductor and hard disk memory, but also with different ways to manufacture and assembly them, and with their application to meet market requirements. It also provides an introduction to the epistemological issues arising in defining the process of remembering, as well as an overview on human memory, and an interesting excursus about biological memories and their organization, to better understand how the best memory we have, our brain, is able to imagine and design memory. (orig.)

Campardo, Giovanni [Numonyx, Agrate Brianza Milano (Italy); Tiziani, Federico; Iaculo, Massimo (eds.) [Micron, Arzano Napoli (Italy)

2011-07-01

394

Memory mass storage  

CERN Document Server

Memory Mass Storage describes the fundamental storage technologies, like Semiconductor, Magnetic, Optical and Uncommon, detailing the main technical characteristics of the storage devices. It deals not only with semiconductor and hard disk memory, but also with different ways to manufacture and assembly them, and with their application to meet market requirements. It also provides an introduction to the epistemological issues arising in defining the process of remembering, as well as an overview on human memory, and an interesting excursus about biological memories and their organization, to b

Campardo, Giovanni; Iaculo, Massimo

2011-01-01

395

Storage Time of Peda  

OpenAIRE

Peda is one of fish based products. Peda can be further processed into a flavor enhancer that is used in various cuisines. Storage time needs to be evaluated since the processing of fishery product damages so easily and shorten its storage time. This research was carried out to determine the flavor enchancer???s storage periode and to enlarge the scale production of peda from raw fish. The parameters used in this research is the proximate analysis, they were stored for 8 weeks by packaging w...

Fitriani U.

2011-01-01

396

Effect of ionizing whole-body irradiation on the primary and secondary antibody reaction of cows to injection of human gamma globulin  

International Nuclear Information System (INIS)

In 3 experiments 29 cows were exposed to whole-body irradiation, using 9 MeV X-rays of a linear accelerator, with doses of 1.50 and 2.00 Gy or 60Co gamma rays with a dose of 2.75 Gy, as a midline dose. 2 weeks prior to irradiation the first immunization was applied using human gamma globulin. 4 or 5 weeks after irradiation a second immunization was carried out. The antibody titres were investigated. The irradiation failed to affect the antibody titres after the first immunization. After the second immunization the antibody titres of the irradiated animals remained diminished significantly (? = 0.05). This has been attributed to a damage of the memory cell pool. (author)

397

Effect of triiodothyronine (L-T/sub 3/) and weight loss on sex-hormone binding globulin (SHBG) in hirsute obese women  

Energy Technology Data Exchange (ETDEWEB)

The effect of a 60-day period of treatment with L-T/sub 3/ (200 ..mu..g/day) was evaluated in hirsute obese women and normal controls. The obese patients were also submitted to a low calorie high protein diet. Mean initial weight significantly declined with a significant lowering of the mean DHEA-S level. There were changes in serum T/sub 3/ which rose in both obese and normal patients. Serum sex-hormone binding globulin levels at baseline studies were significantly higher in normal controls than in obese patients. At the end of the 60-day period of L-T/sub 3/ administration it was observed in both groups a 2-4 fold increase in serum SHBG levels but normal controls had significantly higher mean values than obese individuals.

Cavaliere, H.; Medeiros Neto, G.A. (Sao Paulo Univ. (Brazil). Faculdade de Medicina)

1984-06-01

398

The effect of triiodothyronine (L-T3) and weight loss on sex-hormone binding globulin (SHBG) in hirsute obese women  

International Nuclear Information System (INIS)

The effect of a 60-day period of treatment with L-T3 (200 ?g/day) was evaluated in hirsute obese women and normal controls. The obese patients were also submitted to a low calorie high protein. Mean initial weight significantly declined with a significant lowering of the mean DHEA-S leves. There was changes in serum T3 rose in both obese and normal patients. Serum sex-hormone binding globulin levels at baseline studies were significantly higher in normal controls than in obese patients. At the end of de 60-day period of L-T3 administration it was observed in both groups a 2-4 fold increase in serum SHBG levels but normal controls had significantly higher mean values than obese individuals. (M.A.C.)

399

Association of the pituitary-testicular axis function and sex hormone-binding globulin with melatonin secretion in morbidly obese men  

International Nuclear Information System (INIS)

A possible relationship between melatonin (MEL) secretion and pituitary-testicular function as well as the circadian rhythmicity of serum MEL, lutropin (LH), folitropin (FSH), estradiol (E2), total testosterone (T) and sex hormone-binding globulin (SHBG) were evaluated in 16 men with the primary obesity (body mass index - BMI > 43 kg/m2; waist-to-hip circumference ratio - WHR > 1.0) and in 17 healthy volunteers with normal body weight. The mean 24-h MEL level was significantly higher in obese patients than in healthy control individuals. Moreover, all obese men showed some abnormalities of MEL circadian pattern such as decreased ratio between day and night MEL levels, abnormal secretory peaks during the light hours and lower interindividual variability for timing amplitude. Abnormal circadian variations of MEL were associated with reduced 24-h mean values of LH, FSH, T and SHBG, whereas E2 levels were elevated. (author). 49 refs, 4 figs, 2 tabs

400

Intravenous and standard immune serum globulin preparations interfere with uptake of /sup 125/I-C3 onto sensitized erythrocytes and inhibit hemolytic complement activity  

Energy Technology Data Exchange (ETDEWEB)

Antibody-sensitized sheep erythrocytes were used as a model to determine the effects of therapeutic immune serum globulin (ISG) preparations on the ability of this particulate activator to fix C3 and initiate hemolysis. Both standard and intravenous forms of ISG inhibit uptake of /sup 125/I-C3, presumably by competing for the deposition of ''nascent'' C3b molecules onto the erythrocytes. Both forms of ISG also inhibit hemolytic activity of whole serum or purified complement components. The inhibition appears to be a specific property of IgG itself, since similar inhibition was not caused by equivalent concentrations of human serum albumin, and was not affected by the buffer in which the ISG was dissolved. Interference with C3 uptake onto antibody-sensitized platelets and/or inhibition of hemolytic complement activity could contribute to the efficacy of high dose intravenous ISG in idiopathic thrombocytopenic purpura.

Berger, M.; Rosenkranz, P.; Brown, C.Y.

1985-02-01

401

{alpha}{sub 2u}-globulin nephropathy: Review of the cellular and molecular mechanisms involved and their implications for human risk assessment  

Energy Technology Data Exchange (ETDEWEB)

This paper reviews what is known about the induction of {alpha}{sub 2u}-globulin nephropathy and carcinogenesis. This unique male-rat-specific disease is associated with exposure to an ever-increasing number of chemicals. The processes leading to nephropathy and renal cancer are among the best-understood mechanisms for non-genotoxic chemicals and strongly support that it is a male-rat-specific process that is not relevant for human risk assessment. Nevertheless, the data available for individual chemicals vary greatly. This necessitates a case-by-case analysis of the available data when determining the relevance for humans of this chemically induced renal disease in male rats. 66 refs. 1 tab.

Swenberg, J.A. [Univ. of North Carolina, Chapel Hill, NC (United States)

1993-12-01

402

Sex hormone-binding globulin influences gene expression of LNCaP and MCF-7 cells in response to androgen and estrogen treatment.  

Science.gov (United States)

Sex hormone-binding globulin (SHBG), a plasma protein that binds androgens and estrogens, also participates in the initial steps of a membrane-based steroid signaling pathway in human prostate and breast. We have recently shown that SHBG is expressed at the mRNA and protein levels in the prostate and breast. In this study, we addressed whether locally expressed SHBG: (1) Functions to regulate activation of membrane-based steroid signaling and (2) influences activation of the androgen (AR) and estrogen (ER) receptors. Using microarray analysis, we identified specific genes that are influenced by SHBG expression in LNCaP and MCF-7 cells in a manner consistent with each of these properties. These findings suggest that locally expressed SHBG can play a functional role in the steroid responsiveness of prostate and breast cells through multiple signaling pathways and that perturbations in local SHBG expression could contribute to prostate and breast cancer. PMID:18497082

Kahn, Scott M; Li, Yu-Hua; Hryb, Daniel J; Nakhla, Atif M; Romas, Nicholas A; Cheong, Janice; Rosner, William

2008-01-01

403

[Effective treatment combining antithymocyte globulin, cyclosporin A, and granulocyte colony-stimulating factor for atypical paroxysmal nocturnal hemoglobinuria accompanied by bone marrow hypoplasia].  

Science.gov (United States)

A 25-year-old man was admitted for evaluation of pancytopenia on May 2, 1997. On admission, he had pancytopenia with a normal reticulocyte count. Bone marrow aspirate specimens displayed a normal karyotype and hypocellularity without myelodysplasia. Although total bilirubin and lactate dehydrogenase levels were within their normal ranges, the haptoglobin level was low; additionally, two-color flow cytometric analysis determined that 3.3% of erythrocytes were double-negative for CD55 and CD59 expression. Atypical paroxysmal nocturnal hemoglobinuria with bone marrow hypoplasia was diagnosed. Because initial treatment with cyclosporin A was not effective, the patient was subsequently given a combination of antithymocyte globulin, cyclosporin A, and granulocyte colony-stimulating factor. Although the pancytopenia subsided, the percentage of double-negative erythrocytes in the patient's blood remained almost unchanged compared to findings obtained on admission. PMID:10222633

Noji, H; Shichishima, T; Ishikawa, S; Kai, T; Saitoh, Y; Maruyama, Y

1999-03-01

404

Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures  

Directory of Open Access Journals (Sweden)

Full Text Available Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L. cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC. Seed storage at room temperature reduced viability (to 2% and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered.

Agnieszka I. Piotrowicz-Cie?lak

2008-09-01

405

Storage-ring FEL for the vuv  

International Nuclear Information System (INIS)

A free-electron laser for the vuv operating in a storage ring requires an electron beam of high density and low energy spread and a short wavelength, narrow-gap undulator. These conditions tend to produce longitudinal and transverse beam instabilities, excessive beam growth through multiple intrabeam scattering, and a short gas-scattering lifetime. Passing the beam only occasionally through the undulator in a by-pass straight section, as proposed by Murphy and Pellegrini, allows operation in a high-gain, single-pass mode and a long gas-scattering lifetime. Several storage ring designs have been considered to see how best to satisfy the several requirements. Each features a by-pass, a low-emittance lattice, and built-in wigglers for enhanced damping to counteract the intra-beam scattering. 15 references, 3 figures, 2 tables

406

T-cell-replete haploidentical HSCT with low-dose anti-T-lymphocyte globulin compared with matched sibling HSCT and unrelated HSCT.  

Science.gov (United States)

We developed an approach of T-cell-replete haploidentical hematopoietic stem cell transplantation (HSCT) with low-dose anti-T-lymphocyte globulin and prospectively compared outcomes of all contemporaneous T-cell-replete HSCT performed at our center using matched sibling donors (MSDs), unrelated donors (URDs), and haploidentical related donors (HRDs). From 2008 to 2013, 90 patients underwent MSD-HSCT, 116 underwent URD-HSCT, and 99 underwent HRD-HSCT. HRDs were associated with higher incidences of grades 2 to 4 (42.4%) and severe acute graft-versus-host disease (17.2%) and nonrelapse mortality (30.5%), compared with MSDs (15.6%, 5.6%, and 4.7%, respectively; P < .05), but were similar to URDs, even fully 10/10 HLA-matched URDs. For high-risk patients, a superior graft-versus-leukemia effect was observed in HRD-HSCT, with 5-year relapse rates of 15.4% in HRD-HSCT, 28.2% in URD-HSCT (P = .07), and 49.9% in MSD-HSCT (P = .002). Furthermore, 5-year disease-free survival rates were not significantly different for patients undergoing transplantation using 3 types of donors, with 63.6%, 58.4%, and 58.3% for MSD, URD, and HRD transplantation, respectively (P = .574). Our data indicate that outcomes after HSCT from suitably matched URDs and HRDs with low-dose anti-T-lymphocyte globulin are similar and that HRD improves outcomes of patients with high-risk leukemia. This trial was registered at www.chictr.org (Chinese Clinical Trial Registry) as #ChiCTR-OCH-12002490. PMID:25214441

Luo, Yi; Xiao, Haowen; Lai, Xiaoyu; Shi, Jimin; Tan, Yamin; He, Jingsong; Xie, Wanzhuo; Zheng, Weiyan; Zhu, Yuanyuan; Ye, Xiujin; Yu, Xiaohong; Cai, Zhen; Lin, Maofang; Huang, He

2014-10-23

407

Fuel storage rack  

International Nuclear Information System (INIS)

If fuel enrichment degree and reactivity are increased remarkably with an aim of effective utilization of fuels in a nuclear power plant, it may become difficult to ensure subcriticality for a spent fuel storage rack now in use. It may be considered to replace the already disposed fuel storage rack with other rack of sufficient subcriticality, however, there are disadvantages that operators suffer from radiation exposure during handling of a fuel pool or fuel storage rack contaminated with radioactivity and the replacement operation needs a considerable cost. In view of the above, neutron absorbing plates are disposed detachably on the inner surface of the main body of the fuel storage rack. They can be disposed by using cranes, etc. from the outside of the fuel pool while storing spent fuels as they are. Accordingly, it is not only economical in view of continuous usage and easy operation but also the operator's exposure dose can be reduced. (N.H.)

408

Integrated waste storage plan  

International Nuclear Information System (INIS)

The purpose of this document is to assess the Portsmouth Gaseous Diffusion Plant (PORTS) Uranium Enrichment process and Environmental Restoration waste storage integration, assuming no off-site shipments other than currently approved avenues and no off-site disposal

409

Costs of Archival Storage  

DEFF Research Database (Denmark)

This paper presents an analysis of the cost of archival storage. The study is part of a project conducted by The Danish National Archives, The Royal Library, and The State and University Library to develop a generic cost model for digital preservation (CMDP). The purposes of the study were to determine the costs of establishing and maintaining a preservation solution destined for long-term preservation of digital materials and to develop a tool capable of doing this operation. In order to fulfill the purposes, the project employed a combination of own and external experience as well as the OAIS Reference Model as a framework to fully understand and identify the cost critical activities of bit-preservation as described in Archival Storage. We found that the costs of Archival Storage are obviously closely linked to the data volume, but also to the required preservation quality, especially with regard to the required number of copies and the type of storage solution.

BØgvad Kejser, Ulla

2012-01-01

410

Storage of strawberry pollen  

Directory of Open Access Journals (Sweden)

Full Text Available This study was carried out to determine storage ability of strawberry pollen at different temperatures for three different strawberry cultivars 'Aliso', 'Brio', and 'Cruz'. Strawberry pollen was stored at room temperature (22 ±2°C, +4°C, -4°C and -18°C in stabile humidity conditions. Strawberry poIlen was germinated using the hanging drop method in a 20% sucrose solution. Pollen germination rate increased because of low temperature storage. Pollen stored at room temperature and +4°C, -4°C, and -18°C was kept for 8 months, about one year, and 20 months, respectively. Pollen germination rates decreased as the length of storage period increased. The reaction of all cultivars tested on the duration and temperature of storage was similar.

Rafet Aslanta?

2001-12-01

411

The platelet storage lesion.  

Science.gov (United States)

The gradual loss of quality in stored platelets as measured collectively with various metabolic, functional, and morphologic in vitro assays is known as the platelet storage lesion. With the advent of pathogen reduction technologies and improved testing that can greatly reduce the risk for bacterial contamination, the platelet storage lesion is emerging as the main challenge to increasing the shelf life of platelet concentrates. This article discusses the contribution of platelet production methods to the storage lesion, long-established and newly developed methods used to determine platelet quality, and the significance for clinical transfusion outcome. Highlighted are the novel technologies applied to platelet storage including platelet additive solutions and pathogen inactivation. PMID:20513565

Devine, Dana V; Serrano, Katherine

2010-06-01

412

Hydrogen storage using borohydrides  

International Nuclear Information System (INIS)

The possibilities of hydrogen storage using borohydrides are presented and discussed specially in regard of the recoverable hydrogen amount and related to the recovering conditions. A rapid analysis of storage possibilities is proposed taking in account the two main ways for hydrogen evolution: the dehydrogenation obtained through thermal decomposition or the hydrolysis of solids or solutions. The recoverable hydrogen is related to the dehydrogenation conditions and the real hydrogen useful percentage is determined for each case of use. The high temperature required for dehydrogenation even when using catalyzed compounds lead to poor outlooks for this storage way. The hydrolysis conditions direct the chemical yield of the water consuming, and this must be related to the experimental conditions which rule the storage capacity of the 'fuel' derived from the borohydride. (authors)

413

Storage - Research Councils UK  

SCPinfonet

News, Events and Publications ... This will result in significant differences in the \\supply and demand patterns and will therefore present ... The Energy Storage \\Research Network brings together researchers from academic, industrial and \\policy ...

414

Wet storage integrity update  

International Nuclear Information System (INIS)

This report includes information from various studies performed under the Wet Storage Task of the Spent Fuel Integrity Project of the Commercial Spent Fuel Management (CSFM) Program at Pacific Northwest Laboratory. An overview of recent developments in the technology of wet storage of spent water reactor fuel is presented. Licensee Event Reports pertaining to spent fuel pools and the associated performance of spent fuel and storage components during wet storage are discussed. The current status of fuel that was examined under the CSFM Program is described. Assessments of the effect of boric acid in spent fuel pool water on the corrosion and stress corrosion cracking of stainless steel and the stress corrosion cracking of stainless steel piping containing stagnant water at spent fuel pools are discussed. A list of pertinent publications is included. 84 references, 21 figures, 11 tables

415

Nuclear Materials Storage  

International Science & Technology Center (ISTC)

Creation of System for Storage, Operative Control and Physical Protection of Nuclear Materials and Ampoule Sources of Ionizing Radiation (ASIR) on "Baikal-1" stand Complex Meeting the International Requirements of Radioactive Materials Control...

416

Catalysis for Hydrogen Storage  

Energy Technology Data Exchange (ETDEWEB)

The U.S. Department of Energy has recently issued a Grand Challenge in Hydrogen Storage. The ambitious goals of this challenge demand the discovery of new hydrogen storage materials and new catalysts for H2 release as well as regeneration. We have embarked upon a study of amine borane complexes to meet this challenge. Below we describe one aspect of our research on the hydrogen release from NH3BH3 using a homogeneous ruthenium catalyst.

Linehan, John C.; Autrey, Thomas; Fulton, John L.; Chen, Yongsheng; Balasubramanian, Mahalingam

2005-09-01

417

Storage of strawberry pollen  

OpenAIRE

This study was carried out to determine storage ability of strawberry pollen at different temperatures for three different strawberry cultivars 'Aliso', 'Brio', and 'Cruz'. Strawberry pollen was stored at room temperature (22 ±2°C), +4°C, -4°C and -18°C in stabile humidity conditions. Strawberry poIlen was germinated using the hanging drop method in a 20% sucrose solution. Pollen germination rate increased because of low temperature storage. Pollen stored at room temperature and +4°C, -...

Rafet Aslanta?; Lütfi Pirlak

2001-01-01

418

Developing new transportable storage casks for interim dry storage  

International Nuclear Information System (INIS)

Transportable storage metal casks are to be consistently used during transport and storage for AFR interim dry storage facilities planning in Japan. The casks are required to comply with the technical standards of regulations for both transport (hereinafter called ''transport regulation'') and storage (hereafter called ''storage regulation'') to maintain safety functions (heat transfer, containment, shielding and sub-critical control). In addition to these requirements, it is not planned in normal state to change the seal materials during storage at the storage facility, therefore it is requested to use same seal materials when the casks are transported after storage period. The dry transportable storage metal casks that satisfy the requirements have been developed to meet the needs of the dry storage facilities. The basic policy of this development is to utilize proven technology achieved from our design and fabrication experience, to carry out necessary verification for new designs and to realize a safe and rational design with higher capacity and efficient fabrication

419

Superconducting magnetic energy storage  

International Nuclear Information System (INIS)

Superconducting inductors provide a compact and efficient means of storing electrical energy without an intermediate conversion process. Energy storage inductors are under development for diurnal load leveling and transmission line stabilization in electric utility systems and for driving magnetic confinement and plasma heating coils in fusion energy systems. Fluctuating electric power demands force the electric utility industry to have more installed generating capacity than the average load requires. Energy storage can increase the utilization of base-load fossil and nuclear power plants for electric utilities. Superconducting magnetic energy storage (SMES) systems, which will store and deliver electrical energy for load leveling, peak shaving, and the stabilization of electric utility networks are being developed. In the fusion area, inductive energy transfer and storage is also being developed by LASL. Both 1-ms fast-discharge theta-pinch and 1-to-2-s slow tokamak energy transfer systems have been demonstrated. The major components and the method of operation of an SMES unit are described, and potential applications of different size SMES systems in electric power grids are presented. Results are given for a 1-GWh reference design load-leveling unit, for a 30-MJ coil proposed stabilization unit, and for tests with a small-scale, 100-kJ magnetic energy storage system. The results of the fusion energy storage and transfer tests are also presented. The common technology base for the systems is discussed

420

Spent nuclear fuel storage  

International Nuclear Information System (INIS)

When a country becomes self-sufficient in part of the nuclear cycle, as production of fuel that will be used in nuclear power plants for energy generation, it is necessary to pay attention for the best method of storing the spent fuel. Temporary storage of spent nuclear fuel is a necessary practice and is applied nowadays all over the world, so much in countries that have not been defined their plan for a definitive repository, as well for those that already put in practice such storage form. There are two main aspects that involve the spent fuels: one regarding the spent nuclear fuel storage intended to reprocessing and the other in which the spent fuel will be sent for final deposition when the definitive place is defined, correctly located, appropriately characterized as to several technical aspects, and licentiate. This last aspect can involve decades of studies because of the technical and normative definitions at a given country. In Brazil, the interest is linked with the storage of spent fuels that will not be reprocessed. This work analyses possible types of storage, the international panorama and a proposal for future construction of a spent nuclear fuel temporary storage place in the country. (author)

421

Globulina beta do líquido cefalorraqueano no prognóstico de processos inflamatórios do sistema nervoso central / The beta-globulin content of the cerebrospinal fluid and the evaluation of the prognosis in inflammatory diseases of the central nervous system  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Foi feita avaliação do comportamento da globulina beta do LCR em processos inflamatórios do SNC e/ou de seus envoltórios no sentido de verificar até que ponto podem ser úteis para o prognóstico as informações obtidas. Essa avaliação foi baseada no fato de a concentração dessa globulina no LCR estar [...] relacionada ao metabolismo do parênquima nervoso, aumentando em condições que acarretem seu sofrimento. Os proteinogramas do LCR de 45 pacientes com processos inflamatórios do SNC e/ou de seus envoltórios, distribuídos em 6 grupos de casos, foram analisados segundo a possibilidade de o processo inflamatório estar acarretando, ou não, sofrimento do parênquima encefálico. A análise dos resultados mostrou que o estudo da concentração da globulina beta no LCR fornece elementos que permitem avaliar o comprometimento do parênquima encefálico nessas condições, aduzindo dados úteis para a avaliação prognóstica. A intensidade do aumento da concentração dessa globulina no LCR era maior do que a do aumento da concentração de albumina nos casos em que o processo inflamatório determinava comprometimento do parênquima encefálico. Abstract in english The CSF proteins of 45 patients with inflammatory diseases of the CNS were studied in order to evaluate the information that the beta-globulin content may bring about the occurrence of involvement of brain tissue. The material was distributed in 6 groups according to the diagnosis of the cases: 10 p [...] atients had spinal cord and/or radicular inflammatory disease, associated or not with leptomeningeal involvement (group 1); 5 had acute leptomeningitis (group 2); in 5 the diagnosis of tuberculous meningo-encephalitis was made (group 3); in 10, of cysticercosis of the CNS (group 4); in 5, of neurosyphilis (group 5); in 10, of encephalitides (group 6). Total protein content was determined by the turbidimetric method of the trichloroacetic acid. The protein fractions were analyzed through paper strip electrophoresis. The results were evaluated in respect to normal values previously reported. The high gamma-globulin content of the CSF in inflammatory processes of the CNS hinders the evaluation of the changes occurring in the content of the other globulins; the interference of such factor is more marked when the inflammatory process is chronic. In such conditions the evaluation of the content of the other globulin fractions is better achieved by comparison with the albumin content, the values reported for each globulin being statistically compared to those obtained for the albumin fraction. By this procedure it was shown that in all 45 cases changes in the alpha-globulin content were not different from that found for the albumin fraction and it was concluded that these data bring no evidences indicating interference of other factors than those related to the blood-CSF barrier for the explanation of the changes in the alpha-globulins content of the samples studied. Significant differences were found in respect to beta and gamma globulins. The changes found in the gamma-globulin support the possibility that the increase of this globulin is conditioned by the local production. Data concerning to beta-globulin in the cases of groups 5 and 6 showed that the increases in the amount of this globulin were more marked than those observed for the albumin. This difference was statistically significant. It brings evidence of participation of a different factor in the explanation of the finding other than those accepted for the albumin fraction. The discussion on the nature of this factor supports the possibility of its relation to the changes in the protein metabolism of the brain, since the damage of the latter was present in the cases of these groups of patients. These data are in agreement to the findings on the changes registered in the content of this globulin in the CSF in degenerative diseases as it is reported in the literature. The changes in the beta-globulin content of the C

A., Spina-França.

1964-03-01

422

Effect of chickpea (Cicer arietinum L. germination on the major globulin content and in vitro digestibility Efeito da germinação de grão-de-bico (Cicer arietinum L. na globulina majoritária e digestibilidade in vitro  

Directory of Open Access Journals (Sweden)

Full Text Available Chickpea seed germination was carried out over a period of 6 days. Little variation in the nitrogen and total globulin content was observed. The major globulin (11 S type showed higher variation after the 4th day of germination. The elution behaviour and distribution of the isolated major globulin fraction on Sepharose CL-6B chromatography showed little modification at the end of germination. On SDS-PAGE the peak eluted from Sepharose CL-6B showed changes in protein bands between 20 and 30 kDa and above 60 kDa, indicating protein degradation during the period. Proteolytic activity was detected in the albumin fraction of the seeds, which increased up to the fourth and then decreased up to the sixth day, when isolated chickpea total globulin and casein were used as substrates. Chickpea flour, isolated albumin and total globulin fractions did not show an increase for in vitro digestibility; however, the isolated major globulin was more susceptible to hydrolysis after germination.A germinação das sementes de grão-de-bico foi acompanhada por um período de 6 dias, no qual pequenas variações nos teores de nitrogênio e globulina total foram registradas. A globulina majoritária (tipo 11 S apresentou maiores variações após o quarto dia de germinação. A natureza e distribuição da fração globulina majoritária isolada na cromatografia em Sepharose CL-6B mostrou pequenas modificações ao final do período de germinação. A eletroforese em gel de poliacrilamida com dodecilssulfato de sódio do pico eluído na cromatografia em Sepharose CL-6B demonstra modificações nas bandas de proteínas entre os pesos moleculares de 20 e 30 kDa e acima de 60 kDa, indicando degradação protéica durante o período. Atividade proteolítica foi detectada na fração albumina da semente que aumentou até o quarto dia, seguido de queda até o sexto dia de germinação, quando da utilização de globulina total isolada da semente e caseína como substratos. Farinha de grão-de-bico, frações albumina e globulina total isoladas não apresentaram aumento na digestibilidade in vitro; entretanto, a fração globulina majoritária isolada foi mais suscetível à hidrólise após germinação.

Guilherme Vanucchi Portari

2005-12-01

423

GAS STORAGE TECHNOLOGY CONSORTIUM  

Energy Technology Data Exchange (ETDEWEB)

Gas storage is a critical element in the natural gas industry. Producers, transmission and distribution companies, marketers, and end users all benefit directly from the load balancing function of storage. The unbundling process has fundamentally changed the way storage is used and valued. As an unbundled service, the value of storage is being recovered at rates that reflect its value. Moreover, the marketplace has differentiated between various types of storage services, and has increasingly rewarded flexibility, safety, and reliability. The size of the natural gas market has increa