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Sample records for semen plasma glycerylphosphorylcholine

  1. Uranium quantification in semen by inductively coupled plasma mass spectrometry.

    Science.gov (United States)

    Todorov, Todor I; Ejnik, John W; Guandalini, Gustavo; Xu, Hanna; Hoover, Dennis; Anderson, Larry; Squibb, Katherine; McDiarmid, Melissa A; Centeno, Jose A

    2013-01-01

    In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2g aliquots of pooled control semen at three different levels of uranium: low at 5 pg/g, medium at 50 pg/g, and high at 1000 pg/g. The detection limit was determined to be 0.8 pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9 pg/g of semen (n=10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (uranium in semen is consistent with known differences in current DU body burdens in these individuals, some of whom have retained embedded DU fragments. PMID:22944582

  2. Influence of boar breeds or hybrid genetic composition on semen quality and seminal plasma biochemical variables.

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    Žaja, Ivona Žura; Samardžija, Marko; Vince, Silvijo; Majić-Balić, Ivanka; Vilić, Marinko; Đuričić, Dražen; Milinković-Tur, Suzana

    2016-01-01

    The enzyme concentrations of seminal plasma are important for spermatozoa metabolism and function in boars. The need has arisen for introducing a biochemical evaluation of semen, along with the usual standard semen analyses. There are no data on the influence of boar breeds on the seminal plasma biochemical variables investigated in this study. Therefore, the objective was to determine the influence of breed and hybrid genetic composition of boars on semen quality and seminal plasma biochemical variables. Semen samples of 27 boars (Swedish Landrace, German Landrace, Large White, Pietrain and Pig Improvement Company hybrid-PIC-hybrid), aged between 1.5 and 3 years, were collected. After evaluation of semen quality, the seminal plasma was separated from the spermatozoa by centrifugation of semen. The seminal plasma was subjected to spectrophotometric analysis to determine alkaline phosphatase (ALP), acid phosphatase (ACP), γ-glutamyltransferase (GGT), creatine kinase (CK) and lactate dehydrogenase (LDH) and to atomic absorption spectrophotometric analysis to measure the concentration of calcium and magnesium. Conventional semen quality variables differed depending on breed and PIC-hybrid genetic composition, though these differences were typically insignificant. In the seminal plasma, significant differences were determined in enzyme activity (ALP, GGT, CK and LDH) and in calcium concentration among boars of different breeds. There are, therefore, differences in semen quality and significant differences in the seminal plasma biochemical variables among boars of different breeds and PIC-hybrid genetic composition. The data and differences in semen variables detected in the present study provide knowledge for enhancing evaluation and monitoring of boar reproductive potential, semen quality and explain the potential causes of boar infertility. PMID:26692346

  3. Efecto de la adicin de plasma seminal en el semen equino descongelado Effect of seminal plasma addition on frozen-thawed equine semen

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    D. Lozano Benito

    2011-09-01

    Full Text Available Antecedentes y objetivos: El semen criopreservado ofrece beneficios adicionales no presentes en el semen refrigerado. Sin embargo, varios factores afectan al xito en la inseminacin artificial con semen congelado de caballos. El objetivo del trabajo es evaluar si la adicin de plasma seminal a diferentes concentraciones, sobre espermatozoides equinos descongelados, afecta a la motilidad espermtica, viabilidad y a nivel de membrana. Material y mtodos: Se utilizaron diferentes razas, cuatro sementales de silla, y dos sementales de tiro. En un primer experimento el semen descongelado se centrifug, mientras en el segundo no se centrifug. A continuacin, se adicion el plasma seminal al 10, 20, 30% suspendido en solucin tampn fosfato y plasma seminal puro (100%. Resultados: En los caballos de silla el plasma seminal no afect a los parmetros estudiados (p>0,05, pero se apreci un posible efecto txico del plasma seminal puro sobre las caractersticas espermticas. En las muestras con plasma seminal de los caballos de tiro, se observaron unos ndices mejores en espermatozoides vivos con acrosoma intacto que en las muestras control. Asimismo se obtuvo un porcentaje menor en espermatozoides reaccionados que en las muestras control, encontrando en esta categora una diferencia significativa (pBackground and objectives: Stallion sperm cryopreservation offers benefits not available in cooled semen. However various factors affect the success of artificial insemination with frozen-thawed equine semen. This study aims to evaluate if adding different concentrations of seminal plasma on frozen-thawed equine spermatozoa affects sperm motility, viability and membrane status. Material and Methods: Different breeds were used; four saddle stallions and two draft stallions. In the first experiment thawed semen was centrifuged and in the second one it was not. Subsequent to that, the spermatozoa resuspended with 10, 20, 30% seminal plasma in phosphate buffered saline and pure seminal plasma (100%. Results: semen parameters of saddle stallions were not affected (p>0,05, but a possible toxic effect of pure seminal plasma was observed on sperm characteristics. Seminal plasma samples in draft breed got better rates in viable sperm with intact acrosome. A lower percentage was also found on spermatozoa with acrosome reaction than in control samples. This category showed signif icant differences (p<0,05. Conclusions: Post-thawing spermatozoa incubation with seminal plasma can stop acrosome reaction, due to the low percentage of spermatozoa suffering true acrosome reaction.

  4. Study on the relationship between the trace protein contents in semen plasma and male fertility

    International Nuclear Information System (INIS)

    Objective: To explore the relationship between the trace protein contents in semen plasma and male fertility. Methods: The semen plasma concentrations of albumin (Alb), ?2-microglobulin (?2-m), ?2-microglobulin (?2-m), TH glycoprotein (THP), immunoglobulin G (IgG), secreting-type immunoglobulin A (SIgA), and ferritin (Fer) were determined with RIA in 22 fertile and 125 sterile males. Results: With the exception of ferritin, the semen plasma contents of all these trace proteins in the sterile individuals were lower than those in the fertile ones and there were significant differences (p2-m, Alb and Fer were positively correlated to the sperm counts. Contents of SIgA and IgG could reflect the local immune status of the genital tract. Determination of the contents of these trace proteins in semen plasma would be helpful in the evaluation and management of male infertility

  5. Semen quality and concentration of soluble proteins in the seminal plasma of Alpine bucks Semen quality and concentration of soluble proteins in the seminal plasma of Alpine bucks

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    Simone Eliza Facione Guimares

    2010-06-01

    Full Text Available It was aimed to study the in vitro seminal quality analyzed by complementary tests and to compare them with physical, morphological and biochemical aspects of male goat semen of the Alpine breed. This experiment took place at the Federal University of Viosa, situated at 2045 S latitude and 4251 W longitude, Southwest of Brazil. It was done during the summer months of January and February, and three adult male goats of the Alpine breed were used in intensive conditions. The semen was collected by artificial vagina method. In all semen samples (45 ejaculates, after the physical and morphological analysis, the hiposmotic test was done. In 24 ejaculates, it were done thermo-resistance test, and in 21 ejaculates it were determined the concentration of total soluble proteins in seminal plasma. The male goats presented difference in the semen physical and morphological aspects, in the hiposmotic test and thermo-resistance test, but they did not presented difference in total soluble proteins concentration in seminal plasma. Results of the slow thermo-resistance test and hiposmotic test were positively correlated (r = 0.60. It was concluded, according to our results, that the concentration of total soluble proteins in seminal plasma can not be used as a parameter to predict the seminal quality of Alpine bucks.It was aimed to study the in vitro seminal quality analyzed by complementary tests and to compare them with physical, morphological and biochemical aspects of male goat semen of the Alpine breed. This experiment took place at the Federal University of Viosa, situated at 2045 S latitude and 4251 W longitude, Southwest of Brazil. It was done during the summer months of January and February, and three adult male goats of the Alpine breed were used in intensive conditions. The semen was collected by artificial vagina method. In all semen samples (45 ejaculates, after the physical and morphological analysis, the hiposmotic test was done. In 24 ejaculates, it were done thermo-resistance test, and in 21 ejaculates it were determined the concentration of total soluble proteins in seminal plasma. The male goats presented difference in the semen physical and morphological aspects, in the hiposmotic test and thermo-resistance test, but they did not presented difference in total soluble proteins concentration in seminal plasma. Results of the slow thermo-resistance test and hiposmotic test were positively correlated (r = 0.60. It was concluded, according to our results, that the concentration of total soluble proteins in seminal plasma can not be used as a parameter to predict the seminal quality of Alpine bucks.

  6. Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp. semen obtained by electroejaculation

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    Yudi

    2011-03-01

    Full Text Available The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor. Semen was collected from 2 anesthetized males (4-10 years by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes, and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa. The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05 during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program.

  7. Efecto del plasma seminal sobre el estado redox del semen equino criopreservado

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    Edison Pizarro L.

    2013-10-01

    Full Text Available Objetivo. Determinar el efecto del plasma seminal sobre la generacin de especies reactivas de oxgeno (ERO y la peroxidacin lipdica de semen equino criopreservado y su asociacin con parmetros de calidad seminal. Materiales y mtodos. El semen de cinco caballos de la raza criollo colombiano (dos eyaculados cada uno, fue criopreservado mediante un protocolo de congelacin rpida, empleando un diluyente leche-yema de huevo, suplementado con 0%, 10% y 20% de plasma seminal equino. En muestras de semen fresco y criopreservado se evalu la generacin de ERO y la peroxidacin lipdica por espectrofluorimetra, y los parmetros de calidad seminal de movilidad progresiva, vitalidad e integridad de membrana, mediante microscopia de contraste de fase. Para el anlisis estadstico se ajustaron modelos mixtos y se realizaron anlisis de regresin y correlacin. Resultados. Se hallaron promedios post-descongelacin de movilidad progresiva, vitalidad e integridad de membrana de 37.8%20.2, 50.6% 14.6 y 37.8% 15.5, respectivamente. Para el semen fresco y criopreservado suplementado con 0%, 10% y 20% de plasma seminal, los promedios de produccin de ERO (URF fueron de 13.3410.7, 16.15 13.5, 17.32 16 y 22.98 19.4, respectivamente; mostrando un incremento estadsticamente significativo (p?0.05 en la produccin de ERO por efecto de la criopreservacin y la suplementacin con plasma seminal. Los promedios de peroxidacin lipdica (nmolMDA/ml para estos mismos tratamientos, fueron de 0.41 0.25, 0.720.37, 0.51 0.29 y 0.470.26, respectivamente; mostrando una reduccin significativa (p?0.05 de la peroxidacin lipdica del semen suplementado con 10% y 20% de plasma seminal, respecto al semen no suplementado (0%. Conclusiones. El plasma seminal reduce la peroxidacin lipdica del semen equino criopreservado.

  8. Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan

    OpenAIRE

    Demianus Ferdinand Souhoka; Michel Johan Matatula; Wilmientje Marlene Mesang-Nalley; Muhammad Rizal

    2009-01-01

    The purpose of this research was to examine the effect of lactose in Tris extender on theviability of Etawah crossbreed goat spermatozoa which is preserved in the plasma using Prianganram seminal plasma at 3–5oC. Semen was collected using artificial vagina once a week. Freshsemen was divided into four tubes, and centrifuged at 3,000 RPM for 30 min. Supernatant wasremoved and replaced which an equal volume with Priangan ram seminal plasma. Semen in firsttube was diluted with Tris extender-20% ...

  9. Relationship of zinc concentrations in blood and seminal plasma with various semen parameters in infertile subjects

    International Nuclear Information System (INIS)

    To find out relationship of zinc concentrations in blood and seminal plasma with various semen parameters between fertile and infertile men. (JPMC), Karachi and Department of Biochemistry. Basic Medical Sciences Institute, JPMC, Karachi. Fifty eight primary infertile male subjects, without any treatment, who had regular unprotected intercourse for at least 12 months without conception with their partners, aged 20-40 years, were selected from Infertility Clinic Jinnah Postgraduate Medical Center, Karachi. After semen analyses they were grouped as, oligospermic (30), and azoospermic (28). Twenty five known fertile male selected from general population and after semen analysis were taken as normospermic control group. Semen analyzed according to WHO criteria. Serum and seminal plasma zinc were estimated by 5Br. PAPS Colorimetric method. This study showed significant difference in serum and seminal zinc levels in normospermic, oligospermic (p<0.05) and azoospermic (p<0.005). Seminal plasma zinc showed a positive correlation with sperm count and negative with sperm motility in normospermic and oligospermic and negative correlation with volume, pH, WBC concentration in all three groups. There was no correlation found with sperm morphology. On the basis of the findings of this study and those of other reports, zinc may contribute to fertility through its significant effects on various semen parameters. It seems that the estimation of seminal plasma zinc may help in investigation and treatment of infertile males. (author)

  10. Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

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    Bruno Fagundes

    2011-07-01

    Full Text Available Difficulties in obtaining equine frozen semen with potential fertility are recognized. This study was designed to investigate the effect of seminal plasma on frozen/thawing of eight stallion semen from different breed using the following treatments: Seminal plasma with ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender; Part of seminal plasma with proteins under 10 kDa on frozen extender; Conventional freezing, using whole seminal plasma on frozen extender. Using the parameter of 30% of seminal motility post-thawing as index of good freezability, it was verified an increased percentage of stallions that presented good freezability when semen was frozen with seminal plasma containing ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender. These results, suggested the use of seminal plasma concentrated proteins from own stallion to freezing/thawing semen.

  11. Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan

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    Demianus Ferdinand Souhoka

    2009-09-01

    Full Text Available The purpose of this research was to examine the effect of lactose in Tris extender on theviability of Etawah crossbreed goat spermatozoa which is preserved in the plasma using Prianganram seminal plasma at 3–5oC. Semen was collected using artificial vagina once a week. Freshsemen was divided into four tubes, and centrifuged at 3,000 RPM for 30 min. Supernatant wasremoved and replaced which an equal volume with Priangan ram seminal plasma. Semen in firsttube was diluted with Tris extender-20% egg yolk without lactose (control. Semen in second,third, and fourth tubes were diluted with Tris extender-20% egg yolk and 0.3% (0.3 g per 100 mlextender (L0.3, 0.6% (L0.6, and 1.2% (L1.2 lactose, respectively. Diluted-semen were stored inrefrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS,live spermatozoa (LS, and intact plasma membrane (IPM were evaluated daily during storage at3–5oC for four days. The data were analyzed by usi analysis of variance (ANOVA. Means werecompared significant difference test at 0.05 significant level. Results of this study showed thatmean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS,spermatozoa abnormal, and IPM of Etawah crossbreed goat fresh semen were 0.68 ml, cream,thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12%, and 84%, respectively. Addition oflactose in Tris extender could maintain the quality of semen during preservation. At day-5 ofstorage, the percentages of MS, LS, and IPM for treatment L0.6 (40, 55.6, and 53.8% weresignificantly (P<0.05 higher than treatment L1.2 (36, 50.2, and 51.8%, treatment L0.3 (34, 51.2,and 48.4%, and control (29, 41.4, and 41.8%. In conclusion, addition of 0.6% lactose in Trisextender is the best dose in maintaining the quality of Etawah crossbreed goat spermatozoa, andcould be maintaining percentage of motile spermatozoa 40% for four days after preserved at 3–5oC.

  12. Semen characteristics and selected biochemical markers of canine seminal plasma in various seasons of the year.

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    Strzeżek, R; Szemplińska, K; Filipowicz, K; Kordan, W

    2015-01-01

    The aim of this study was to evaluate the influence of season on selected qualitative semen characteristics and biochemical markers of canine seminal plasma. Whole ejaculates were collected from 5 crossbred dogs aged 2-8 years. The study covered a period of one year divided into four seasons: spring (March, April, May), summer (June, July, August), autumn (September, October, November) and winter (December, January, February). Semen samples were subjected to macroscopic and microscopic analyses to determine semen volume, total sperm counts and sperm morphology parameters. The study also involved the determination of sperm motility parameters (CASA system), sperm plasma membrane integrity (SPMI, fluorescent staining SYBR-14/PI), sperm mitochondrial membrane potential (MMP, fluorescent staining JC-1/PI) and the ATP content of sperm cells. Total protein content (TPC) and the activity of alkaline phosphatase (AP) and acid phosphatase (AcP) were determined in biochemical analyses of seminal plasma. No significant differences in ejaculate volume, SMPI or ATP content of sperm cells were observed between seasons. The highest total sperm counts were reported in ejaculates acquired in summer and autumn. The lowest MMP values were determined in summer ejaculates. No significant differences in sperm motility (MOT) were observed throughout the experiment, but ejaculates collected in autumn and winter were characterized by the highest progressive motility (PMOT). AP activity and TPC were not significantly affected by season. However, AcP activity levels were significantly lower in autumn than in the remaining seasons. Seasonal variations in the analyzed macroscopic and microscopic parameters of ejaculates and biochemical markers of seminal plasma did not exert a clear negative effect on the quality of canine semen. PMID:25928905

  13. Primary study on the clinical significance of measurement of epidermal growth factor (EGF) and NPY concentrations in human semen plasma

    International Nuclear Information System (INIS)

    Objective: To investigate the difference between the semen plasma contents of EGF and NPY in fertile and non-fertile males with the relevant sperm count and motility. Methods: Semen plasma contents of EGF and NPY were determined with RIA in 110 non-fertile males. Simultaneous semen analysis revealed (1) Group A, n=45, with normal sperm count, (2) Group B, n=34 low sperm count (0-20) x 106/ml and (3) Group C n=31, with aspermia. White blood cell/HPF was examined in all the semen specimens and sperm motile rate and motility were examined in Group A specimens. Results: The semen plasma contents of EGF and NPY in non-fertile males were significantly higher than those in fertile males (P 1 x 106/ml) were significantly lower than those in specimens with more white blood cells (P<0.05). Conclusion: Higher semen plasma contents of EGF and NPY might exert toxic effect on the sperms, contributing to the development of infertility. (authors)

  14. Effect of seminal plasma and egg yolk concentration on freezability of goat semen

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    Valéria da Silva Ferreira

    2014-10-01

    Full Text Available The objective of this study was to evaluate the effects of egg yolk and seminal plasma on the viability of cryopreserved goat semen. To this end, four fertile Saanen bucks, aged between 10 months and 1 year, and weighing 18 to 25 kg, were used. Semen was collected from each buck by the artificial vagina method at the end of breeding season (June-July. The extender used was the yolk citrate, which was split into two equal aliquots: 5% egg yolk (2.5 mL egg yolk: 47.5 mL citrate solution were added to one of the samples and 10% egg yolk (5.0 mL egg yolk: 45.0 mL citrate solution were added to another. The sperm motility and vigor after thawing and post thermal resistance test (TRT were evaluated and the data were subjected to analysis of variance and means were compared by the F test at 5.0% probability. The observed values for motility and vigor after thawing and post thermal resistance test (TRT, fast and slow, according to the presence of seminal plasma and egg yolk percentage were: 5% egg yolk with plasma (25.0% and 3.3; 1.60% and 0.7; 12.36% and 1.6, respectively; 5% egg yolk without plasma (23.61% and 3.1; 1.25% and 0.2; 9.93% and 1.3, respectively; 10% egg yolk with plasma (30.8% and 3.3; 4.4% and 1.9; 19.5% and 2.7, respectively; and 10% egg yolk without plasma (13.4% and 2.5; 4.1% and 0.5; 17.0% and 1.0, respectively. There were significant differences between the analyzed data in relation to semen with or without plasma at different percentages of egg yolk, and the group that presented the best results was 10% egg yolk citrate in extender with plasma. The presence of seminal plasma and higher concentration of egg yolk in extender provide a higher viability of cryopreserved goat semen.

  15. Comparison of Plasma Testosterone, Thyroid Levels, and Semen Characteristics of Local and Cross of Improved Awassi Strains in Jordan

    OpenAIRE

    AYOUB, Mohamed; TABBAA, Mohammad Jihad; Alnimer, Mufeed; Ababneh, Mohammad

    2012-01-01

    This study conducted to evaluate semen quality and plasma testosterone (T), tetra-iodothyronine (T), and tri­iodothyronine (T) concentrations for different strains of Awassi sheep of different ages in Jordan. Physical traits, semen characteristics and blood samples were assessed in 12 local Awassi rams (6 matures and 6 yearlings) and the 7 crosses of improved Awassi rams (4 matures and 3 yearlings) from the beginning of April until the end of September at the Khanasry Station in Jordan. Local...

  16. Relationship between seminal plasma zinc and semen quality in a subfertile population

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    DMAB Dissanayake

    2010-01-01

    Full Text Available Rationale : Current knowledge on the relationship between seminal zinc levels and different parameters of human semen is inconsistent. Objectives : To assess the relationship between seminal plasma zinc and semen quality using two markers; zinc concentration (Zn-C and total zinc per ejaculate (Zn-T. Design : The study was carried out as a cross-sectional study. Subjects and Methods : Semen parameters of 152 healthy men undergoing evaluation for subfertility were assessed. Seminal plasma zinc levels were determined using flame atomic absorption spectrometry. Zn-C, expressed as μg/mL, was multiplied by ejaculated volume to calculate Zn-T. Mann Whitney U test and Chi-square test were used to compare the zinc levels between different seminal groups when appropriate. Correlations were observed with Pearson′s correlation of coefficient. Analysis was carried out using SPSS 10.0 for windows software. Results : Zn-C was low in 23 (15% samples, while in 32 (21% of the samples Zn-T was abnormal. The number of subnormal samples was high in the low-zinc groups compared with the normal-zinc groups, 15 vs. 8 (P > 0.05 for Zn-C and 28 vs. 4 (P < 0.001 for Zn-T. Zn-C was significantly high in the asthenozoospermics compared with the normal motile group; 138.11 μg/mL (83.92 vs. 110.69 11 μg/mL (54.59 (P < 0.05. Zn-T was significantly low in samples with hyperviscosity compared with samples with normal viscosity; 220.06 μg (144.09 vs. 336.34 μg (236.33 (P < 0.05. Conversely, Zn-T was high in samples with low viability compared with those with normal viability; 437.67 μg (283.88 vs. 305.15 μg (221.19 (P < 0.05. Weak correlations were found between Zn and some semen parameters. However, the correlation was negative between pH and Zn-C (r = -0.193, P < 0.05 as well as Zn-T (r = -0.280, P < 0.01. On the other hand, correlations were positive between Zn-T and sperm count (r = 0.211, P < 0.05. Conclusion : Count, motility, viability, pH and viscosity are affected by variations of seminal plasma zinc. Seminal plasma Zn-T is the better marker for assessing the relationship between zinc and semen quality.

  17. EFFECT OF PROTEINE CONTENT IN BOAR SEMINAL PLASMA ON THE SPERM MOTILITY IN DILUTED SEMEN STORED FOR 3 DAYS

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    Jelena Api?

    2015-02-01

    Full Text Available Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17oC for 3 days. Progressive motility was significantly higher (p<0.01 in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%. After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01 decreased in relation to this value in native semen from the boars with high (82% to64%, as well from the boars with low protein content in seminal plasma (76% to48%. However, the average diluted semen progressive motility was significantly greater (p<0.01 in the boars with high (64%, compared to the boars with low protein content in seminal plasma (48%. The number of good diluted semen samples (?65% progressive motility, was also significantly (p<0.01 greater in the boars with high (41%, compared to the boars with low protein content in seminal plasma (12%. These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.

  18. Detection of haptoglobin in seminal plasma of Awassi rams and the relation with its level in serum and some semen parameters

    OpenAIRE

    Dhafer M. Aziz; Ahmad K. Ahmad

    2012-01-01

    The study was conducted to detect haptoglobin in seminal plasma (SP-Hp) of Awassi rams and the effect of the breeding season on its concentration, along with determining the correlation with its concentration in serum (S-Hp) and main semen variables. Pre-warmed artificial vagina was used to collect semen samples biweekly from five Awassi rams. Semen samples were evaluated for volume, concentration and sperm motility. Blood samples were collected 10–30 min after semen collection. The concentra...

  19. Effect of seminal plasma vesicular structures in canine frozen-thawed semen.

    Science.gov (United States)

    Goericke-Pesch, S; Hauck, S; Failing, K; Wehrend, A

    2015-12-01

    Membrane vesicles (MVs) in the ejaculate have been identified in various species and are considered to affect membrane fluidity due to their characteristic molecular composition. Addition of MV to human frozen semen has been shown to improve post-thaw motility. Similarly, a beneficial effect has been suggested for frozen equine semen. As post-thaw canine semen quality varies widely between dogs, the aim of our study was to test for the effect of addition of canine MV on post-thaw semen quality in dogs. Semen samples from 10 male dogs were purified from MV and prepared for freezing. In experiment 1, three groups were compared: sperm frozen (1) with MV (S1); (2) without MV, but MV added immediately after thawing (S2); and (3) without MV (C). Semen analysis included computer-assisted sperm analysis of motility parameters immediately after thawing (t0), after 10 (t10) and 30 minutes (t30), % living sperm, % membrane intact, % morphologically normal sperm (all t0 and t30). Computer-assisted sperm analysis motility distance and velocity parameters (all P sperm (P sperm motility analysis was performed at t0, t10, and t30. No differences between MV concentrations were identified, only a significant interaction between effect of treatment and time for progressive motility (P canine MV on post-thaw distance and velocity parameters, whereas at t30 progressive motility, motility parameters and % living sperm were reduced in samples with MV compared to C. The results point to species-specific differences regarding the MV effect on frozen semen and indicate the need for further studies using different semen and MV purification protocols and more frequent analyses. At the moment, addition of MV is not an option to improve post-thaw semen quality in dogs. PMID:26296522

  20. Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and computer-assisted sperm analysis (CASA) semen variables.

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    Kumar, Pradeep; Saini, Monika; Kumar, Dharmendra; Jan, M H; Swami, Dheer Singh; Sharma, R K

    2016-03-01

    The present study is the first to quantify leptin in seminal plasma of buffalo and investigate its relationship with seminal attributes. Ten ejaculates each from 10 Murrah buffalo bulls were collected. Semen quality variables such as semen volume, sperm concentration, sperm abnormalities, membrane integrity, antioxidant enzyme activities (superoxide dismutase, catalase and total antioxidant capacity), malondialdehyde (MDA) concentration, as well as sperm kinetics and motility variables were evaluated. The leptin concentration in serum and seminal plasma were estimated by the ELISA method. Bulls were classified in two groups on the basis of sperm concentration with Group I having >800 million sperm/mL and Group II kinetic and motility variables, sperm membrane integrity and seminal plasma antioxidant enzyme activity. Thus, the data suggest that seminal leptin has a role in spermatogenesis and can be used as a marker for spermatogenesis to predict the capacity of buffalo bulls for semen production. PMID:26796919

  1. L-alpha-glycerylphosphorylcholine inhibits the transfer function of phosphatidylinositol transfer protein alpha.

    Science.gov (United States)

    Komatsu, Hiroaki; Westerman, Jan; Snoek, Gerry T; Taraschi, Theodore F; Janes, Nathan

    2003-12-30

    Phosphatidylinositol transfer protein alpha (PITP-alpha) is a bifunctional phospholipid transfer protein that is highly selective for phosphatidylinositol (PtdIns) and phosphatidylcholine (PtdCho). Polar lipid metabolites, including L-alpha-glycerylphosphorylcholine (GroPCho), increasingly have been linked to changes in cellular function and to disease. In this study, polar lipid metabolites of PtdIns and PtdCho were tested for their ability to influence PITP-alpha activity. GroPCho inhibited the ability of PITP-alpha to transfer PtdIns or PtdCho between liposomes. The IC(50) of both processes was dependent on membrane composition. D-myo-inositol 1-phosphate and glycerylphosphorylinositol modestly enhanced PITP-alpha-mediated phospholipid transfer. Choline, phosphorylcholine (PCho), CDP-choline, glyceryl-3-phosphate, myo-inositol and D-myo-inositol 1,4,5-trisphosphate had little effect. Membrane surface charge was a strong determinant of the GroPCho inhibition with the inhibition being greatest for highly anionic membranes. GroPCho was shown to enhance the binding of PITP-alpha to anionic vesicles. In membranes of low surface charge, phosphatidylethanolamine (PtdEtn) was a determinant enabling the GroPCho inhibition. Anionic charge and PtdEtn content appeared to increase the strength of PITP-alpha-membrane interactions. The GroPCho-enhanced PITP-alpha-membrane binding was sufficient to cause inhibition, but not sufficient to account for the extent of inhibition observed. Processes associated with strengthened PITP-alpha-membrane binding in the presence of GroPCho appeared to impair the phospholipid insertion/extraction process. PMID:14729069

  2. Semen parameters and seminal plasma protein and biochemical profiles of dogs with benign prostatic hyperplasia after botulinum toxin type A intraprostatic injection

    Directory of Open Access Journals (Sweden)

    Tathiana Ferguson Motheo

    2014-06-01

    Full Text Available This study aimed to determine the effects of different concentrations of botulinum toxin type A (BT-A on semen parameters, and seminal plasma biochemical and protein profiles of dogs with benign prostatic hyperplasia (BPH. Eighteen sexually intact male dogs with BPH were randomly divided in three groups, and received an intraprostatic injection of saline solution (control group - CG, 250UI (GI or 500UI (GII of BT-A under transabdominal ultrasound guidance. Semen was collected at baseline, 2, 4 and 8 weeks after treatment. Semen parameters were determined and seminal plasma pH, total protein (TP, total chlorides (TC, calcium (Ca, potassium (K, and sodium (Na concentrations were assessed. One-dimensional sodium dodecyl sulfatepolyacrilamide gel eletrophoresis (SDS- PAGE was performed to determine seminal plasma protein profile. Sperm parameters and seminal plasma pH, TP, TC, Ca and K mean values did not change significantly at any time point and among treated groups (P>0.05. The SDS-PAGE analysis of the pooled fractions identified 31 protein bands with molecular weights ranging from 3.9 to 106.2kDA in all treatment groups during the entire evaluation period. Regardless the used dose, intraprostatic BT-A injection do not alter semen parameters and seminal plasma biochemical and protein profiles of dogs with BPH.

  3. Protenas do plasma seminal de caprinos relacionadas com o ndice pluviomtrico e a qualidade do smen Proteins of goat seminal plasma related with precipitation index and semen quality

    Directory of Open Access Journals (Sweden)

    Andreia Fernandes de Souza

    2009-07-01

    Full Text Available O objetivo deste estudo foi identificar as protenas do plasma seminal de caprinos da raa Alpina Americana criados na regio Nordeste do Brasil que esto relacionadas ao ndice pluviomtrico e qualidade do smen. O smen foi obtido pelo mtodo de vagina artificial a partir de trs reprodutores e foi avaliado quanto aos parmetros macroscpicos e microscpicos. O perfil de protenas do plasma seminal foi realizado por eletroforese bidimensional. Os parmetros volume do smen, integridade do acrossoma e protenas totais evidenciaram diferena significativa (PThe aim of this study was to identify proteins in seminal plasma of goats raised in the Northeast of Brazil related with precipitation index and semen quality. Semen was obtained from three bucks and evaluated to the microscopic and macroscopic parameters. The profile of seminal plasma proteins was performed by analysis of two-dimensional electrophoresis. Volume, acrosome integrity and total proteins had significant difference (P<0.05 between the periods of high (1.7mL, 90.3% and 372g mL-1, respectively and low (1.2mL, 80.3% and 494g mL-1, respectively precipitation index. It was detected during high and low precipitation index, 47 and 49 spots of proteins with molecular weight of 4 to 106kDa and 15 to 97kDa, and isoelectric point of 3.00 to 8.96, and 4.48 to 9.83, respectively. Only in the period of high precipitation index were observed groups of proteins with 13kDa and 45kDa. It can be concluded that semen of Alpine American goats raised in the Northeast of Brazil has best quality when obtained in the period of high precipitation index, which can be attributed to the presence of protein with 13kDa and 45kDa.

  4. [Semen allergy].

    Science.gov (United States)

    Allam, J-P; Haidl, G; Novak, N

    2015-12-01

    A semen allergy is a typeI reaction. Reliable figures about incidence/prevalence are not available. Symptoms can be characterized as local and systemic. After exposure to ejaculate, the patient may experience itching and swelling at points of contact, while systemically it may also lead to generalized urticaria with angioedema or higher grade anaphylaxis. As triggering allergens, substances in seminal plasma (SP) have been identified, which can be SP typical or SP atypical. Reactions against spermatozoa have not yet been clearly proven. With regard to SP-typical allergens, prostate-specific antigen (PSA) has been identified, while for SP-atypical allergens, medications or food allergens have been reported, which apparently accumulate in the SP and can then trigger symptoms in women with existing sensitization. The main criteria for the diagnosis of sperm allergy is freedom from symptoms when condoms are used during intercourse. In addition, skin prick tests and determination of allergen-specific IgE are used. In patients with a desire for children, washed, SP-free spermatozoa can be used for insemination. In addition, desensitization may be considered. PMID:26490774

  5. Identifying useable semen.

    Science.gov (United States)

    Foxcroft, G R; Dyck, M K; Ruiz-Sanchez, A; Novak, S; Dixon, W T

    2008-11-01

    The "predictors of useable semen" used in most commercial AI centers provide a very conservative estimate of the relative fertility of individual boars. Furthermore, the relatively high sperm numbers used in commercial AI practice (usually >3 x10(9) total sperm per dose of extended semen) usually compensate for reduced fertility, as can be demonstrated in some boars when lower numbers of sperm are used for AI. Differences in relative boar fertility are also masked by the widespread use of pooled semen for commercial AI in many countries. However, the need to continually improve the efficiency of pork production, suggests that commercial AI practice should involve increased use of boars with the highest genetic merit for important production traits. Necessarily, this must be linked to the use of fewer sperm per AI dose, fewer inseminations per sow bred, and hence more sows bred by these superior sires. In turn, this requires improved techniques for evaluating semen characteristics directly related to the fertilization process, such as IVM-IVF assays, analysis of seminal plasma protein markers, more discriminatory tests of sperm motility and morphology, with the goal of identifying high-index boars whose fertility is sustained when low numbers of sperm are used for AI. This paper reviews the current status of laboratory-based boar semen evaluation techniques that meet these criteria. PMID:18775561

  6. Crioprotetor para smen de carneiro a base de plasma de gema mantm membrana acrossomal intacta aps a descongelao / Ram semen cryoprotector based on egg yolk plasma maintain the viability of acrosomal membrane

    Scientific Electronic Library Online (English)

    Ivo Walter dos, Santos; Jandui Escario da, Nbrega Junior; Matheus Pedrotti de, Cesaro; Gustavo Freitas, Ilha; Monique Tomazele, Rovani; Paulo Bayard Dias, Gonalves.

    2015-06-01

    Full Text Available O presente estudo teve como objetivo avaliar a capacidade crioprotetora das lipoprotenas de baixa densidade (LDL) presentes no plasma de gema de ovo, adicionado ao trihidroxiaminometano (TRIS) para congelar smen ovino. Trinta e seis ejaculados foram coletados para formar 12 "pool". Cada alquota d [...] e smen foi diluda em TRIS-gema de ovo (TRISG) ou TRIS- plasma de gema de ovo (TRISP) antes de congelar o smen. Para a obteno do plasma da gema de ovo, foi utilizado o mtodo de ultracentrifugao. Aps o descongelamento, no houve diferena entre os dois extensores em relao aos parmetros seminais (motilidade, viabilidade, membrana acrossmica e plasma). No entanto, no Teste de Termo Resistncia Lenta (TTRL - 4h/38C), o smen congelado com TRISP resultou no aumento do nmero de espermatozoides com acrossoma intacto (P Abstract in english The present study aimed to evaluate the cryoprotectant low-density lipoprotein (LDL) present in the plasma of egg yolk added to the extender trihidroxiaminometano (TRIS) for freezing ram semen. Thirty-six ejaculates were collected to form 12 pool. Each aliquot of semen was diluted in TRIS-egg yolk ( [...] TRISG) or TRIS-egg yolk plasma (TRISP) before freezing the semen. The plasma of egg yolk was obtained by ultracentrifugation. After thawing, no difference was detected between the two extenders in relation to seminal parameters (motility, viability, plasma membrane and acrosome). However, in the thermal resistance slow test (4h in a water bath at 38C), the semen frozen with TRISP resulted in higher number of sperm with intact acrosome than those with TRISG (P

  7. Calcium, Magnesium and Total Antioxidant Capacity (TAC in Seminal Plasma of Water Buffalo (Bubalus Bubalis Bulls and their Relationships with Semen Characteristics

    Directory of Open Access Journals (Sweden)

    Mohammad-Hassan Khadem Ansari

    2010-06-01

    Full Text Available In order to determine calcium (Ca, magnesium (Mg content and total antioxidant capacity (TAC of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its Ca and Mg content were estimated and its TAC determined. The Ca and Mg content of the seminal plasma (Mean ± SEM were recorded as 22.36 ± 0.52 mg dl-1 and 11.94 ± 0.36 mg dl-1 respectively, while, its mean TAC value was 1.50 ± 0.02 mmol L-1. The mean Ca value was highly associated with sperm progressive motility, gross motility, viability (P = 0.000 for all, negatively with semen volume (P = 0.01, and with Mg and TAC values (P = 0.000 for both. The mean Mg values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and TAC (P = 0.000 for all and negatively associated with semen volume (P = 0.014. The mean TAC values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and Mg (P = 0.000 for all. For further clarification of these associations, the data was categorized in three groups of excellent (Ex, >90% motile, n = 33, good (Go, 80-89% motile, n = 15 and moderate (Mo, <79% motile, n = 6 according to their percentage of sperm motility. The mean progressive motility in Ex group was 92.24 ± 0.51%, in Go group it was 81.66 ± 0.62 %, and in Mo group it was 71.66 ± 1.05 %. The mean Ca, Mg and TAC values were respectively recorded as 25.12 ± 0.29 mg dl-1, 13.78 ± 0.20 mg dl-1, and 1.57 ± 0.009 mmol L-1 in Ex, 18.74 ± 0.63 mg dl-1, 9.14 ± 0.33mg dl-1, and 1.42 ± 0.044 mmol L-1 in Go, and 17.34 ± 0.18 mg dl-1, 8.06 ± 0.25 mg dl-1, and 1.23± 0.05 mmol L-1 in Mo groups. The associations in groups are discussed. These results show that seminal plasma Ca and Mg content and TAC are associated with semen characteristics, and synergistically have an effect on motility and viability of the spermatozoa after ejaculation, which are important factors in semen fertility.

  8. Concentrations of Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoate (PFOA) and Their Associations with Human Semen Quality Measurements

    OpenAIRE

    Raymer, James H; Michael, Larry C.; Studabaker, William B.; Olsen, Geary W.; Sloan, Carol S.; Wilcosky, Timothy; Walmer, David K.

    2011-01-01

    A total of 256 men were studied to evaluate whether serum concentrations of perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) impacted semen quality or reproductive hormones. Blood and semen were collected and analyzed for perfluorochemicals and reproductive and thyroid hormones. Semen quality was assessed using standard clinical methods. Linear and logistic modeling was performed with semen profile measurements as outcomes and PFOS and PFOA in semen and plasma as explanatory var...

  9. Efeito de protenas do plasma seminal eqino com massa superior a 10 kDa concentradas 10 vezes sobre a congelabilidade do smen Effect of high concentration of protein of the equine seminal plasma on semen cryopreservation

    Directory of Open Access Journals (Sweden)

    Marcus Antonio Pessanha Barreto

    2008-12-01

    Full Text Available Objetivou-se avaliar os efeitos da adio de concentrados de protenas do plasma seminal (PPS no diluente de congelamento sobre a congelabilidade do smen eqino. Foram avaliados trs tratamentos: um controle, no qual o smen foi congelado no diluente Botu-Crio; e outros dois, com adio de 10% ou 20% (v/v de protenas do plasma seminal ao diluente. As maiores mdias de motilidades total e progressiva foram observadas no tratamento controle, que foram superiores s obtidas com adio de 20% de protenas, mas no diferiram das obtidas com adio de 10% de PPS. Os resultados do teste hiposmtico e do nmero de espermatozides vivos obtidos com o congelamento do smen no diluente (controle foram superiores aos encontrados com a adio de 10% de PPS, que, por sua vez, foram melhores que os observados com a adio de 20% de PPS ao diluente. A adio do concentrado de protenas do plasma seminal no melhora os parmetros espermticos do smen eqino.The aim of this study was to evaluate the effect of increasing the concentration of protein of the seminal plasma in the extender used for frozing equine semen. Three treatments were compared: The conventional one, defined by using only the Botu-Crio extender for frozing semen; and other two defined by adding 10% (v/v or 20% (v/v of seminal plasma proteins to Botu-Crio extender. Averages of total and progressive motility were statistically higher in the conventional treatment than in that defined by adding 20% (v/v of seminal plasma proteins but they did not differ from those obtained by adding 10% (v/v of seminal plasma proteins to Botu-Crio extender. The best results for the hypoosmotic test and the number of live spermatozoa were obtained in the conventional treatment, and results for adding 10% (v/v of seminal plasma proteins were better than those obtained by adding 20% (v/v of seminal plasma proteins to Botu-Crio extender. These results indicate that the addition of concentrated protein of the seminal plasma to the extender did not improve the cryopreservation of equine semen.

  10. INAA determination of selenium via sup(77m)Se in plasma, semen and hair samples from beef and dairy bulls

    International Nuclear Information System (INIS)

    Interest in the element selenium with respect to its biological significance has been steadily increasing for the last ten years. Neutron activation analysis has long been used for the accurate determination of selenium in biological samples usually via 75Se. More recently activation analysts having access to high flux reactors with rapid delivery pneumatic tube facilities; have successfully employed sup(77m)Se. This approach, which is much faster, is particularly well suited to the Missouri University Research Reactor (MURR). The specific interest concerning bulls has to do with the involvement of selenium in the reproductive system. Selenium analysis methodology and data on plasma, semen and 22 tissues from both beef and dairy bulls are presented. (author)

  11. Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo Addition of seminal plasma to frozen-thawed semen and pregnancy rate of fixed time inseminated ewes

    Directory of Open Access Journals (Sweden)

    O.R. Prado

    2013-02-01

    Full Text Available Avaliou-se o efeito da adição de plasma seminal ovino ao sêmen descongelado sobre a taxa de prenhez de ovelhas em rebanho comercial. Cento e setenta e quatro ovelhas cruza Texel foram distribuídas em quatro tratamentos: T1 inseminação artificial cervical (IAC com sêmen descongelado (SD diluído em solução tampão fosfato salino (PBS; T2 IAC com SD e adição de plasma seminal ovino; T3 grupo-controle I: IAC com sêmen fresco diluído em PBS; T4 grupo-controle II: inseminação artificial por laparoscopia com SD diluído em PBS. Para indução de cio, utilizaram-se esponjas impregnadas com acetato de medroxiprogesterona (MAP por 12 dias, com aplicação intramuscular de 400 UI de eCG (Novormon® e de 37,5µg de cloprostenol sódico (Sincrocio®, no dia da retirada das esponjas. O aparecimento de cio foi monitorado com rufiões vasectomizados a partir da retirada das esponjas até a inseminação artificial em tempo fixo - 54 a 60 horas. A taxa de prenhez do tratamento com adição de plasma seminal ao sêmen descongelado (7,0% não diferiu (P>0,05 do tratamento sem adição de plasma (4,3%, entretanto foi menor (PThe effect of seminal plasma addition to thawed-frozen ram semen on the pregnancy rate of commercial herd ewes was evaluated. One hundred and seventy-four crossbred Texel sheep were allocated to four treatments: T1 cervical artificial insemination (CAI using frozen-thawed semen (FTS diluted in phosphate buffered saline solution (PBS; T2 CAI using FTS diluted in ovine seminal plasma; T3 control group I: CAI using fresh semen diluted in PBS; T4 control group II: laparoscopic insemination using FTS diluted in PBS. Estrus induction was performed with medroxiprogesterone acetate (MAP impregnated sponges for 12 days, followed by intramuscular injection of 400 IU of eCG (Novormon® and 37.5µg of sodium cloprostenol (Sincrocio® on the day of sponge removal. Estrus was monitorated with vasectomized rams, beginning at the time of the sponge removal until the fixed time artificial insemination - 54 to 60 hours. The pregnancy rate of FTS diluted in seminal plasma treatment (7.0% did not differ (P>0.05 for the treatment without addition of seminal plasma (4.3%, however it was lower (P<0.05 when compared to the pregnancy rate of the cervical inseminated control I group with PBS diluted fresh semen (50.0% and laparoscopic inseminated control group II with PBS diluted FTS (39.4%. The cervical artificial insemination with the addition of seminal plasma to frozen-thawed semen did not increase the pregnancy rate at acceptable values to make this biotechnology useful on commercial herds.

  12. Semen collection using phantom in dromedary camel.

    Science.gov (United States)

    Ziapour, S; Niasari-Naslaji, A; Mirtavousi, M; Keshavarz, M; Kalantari, A; Adel, H

    2014-12-10

    Semen collection is relatively long, unsafe, and tedious procedure in dromedary camel. The innovation of safe, hygienic, and simple approach to collect semen could make great progress in development of AI program in this species. This study investigated two methods of semen collection using phantom and artificial vagina in dromedary camel. Semen was collected using phantom (n = 4 bulls; 26 collections) and artificial vagina (n = 6 bulls; 11 collections) and diluted with INRA96 at the ratio of 1:10. The duration of semen collection, semen parameters, and morphometric features of sperm were evaluated. For specimen collected through phantom and AV, the respected duration of semen collection (411.2 ± 48.19 vs 326 ± 37.05 sec), volume (6.6 ± 0.87 vs 6 ± 1.57 ml), osmolarity (328 ± 1.6 vs 319.4 ± 3.21 mOsm/kg H2O), pH (7.7 ± 0.06 vs 7.9 ± 0.16) of semen, concentration (161.4 ± 44.05 × 10(6)/mL vs 160.2 ± 58.42 × 10(6)/mL), total motility (84.1 ± 1.89 vs 78.3 ± 3.97%), progressive forward motility (45.5 ± 3.69 vs 44.3 ± 6.41%), live percentage (72.2 ± 3.11 vs 76 ± 2.53%), and plasma membrane integrity (61.5 ± 2.49 vs 58.9 ± 4.19%) of sperm were similar (P > 0.05). The number of specimens contaminated with visible particles was greater using AV (72.7%) compared to phantom (0%; P semen due to simplicity, safety, and lack of specimen contamination in dromedary camel. PMID:25449548

  13. ALKALINE PHOSPHATASE ACTIVITY AS A MARKER OF DOG SEMEN FREEZABILITY

    Directory of Open Access Journals (Sweden)

    K. KOSINIAK-KAMYSZ

    2013-12-01

    Full Text Available The investigation was performed to evaluate the dog semen freezability and itsquality after thawing allowing its use for artificial insemination (AI. On the basis ofsperm motility, concentration and alkaline phosphatase (AP activity in semenplasma it was possible to establish that AP activity corresponds with the basic factorof semen examination. Significant statistical differences occurred between thequality of ejaculates which were qualified or disqualified to deep freezing and AI.These results show that AP activity in raw dog semen plasma can be used as amarker for the dog semen qualification for deep freezing and AI with 95%probability of the prognosis of the results.

  14. ALKALINE PHOSPHATASE ACTIVITY AS A MARKER OF DOG SEMEN FREEZABILITY

    Directory of Open Access Journals (Sweden)

    KOSINIAK-KAMYSZ K.

    2007-01-01

    Full Text Available The investigation was performed to evaluate the dog semen freezability and itsquality after thawing allowing its use for artificial insemination (AI. On the basis ofsperm motility, concentration and alkaline phosphatase (AP activity in semenplasma it was possible to establish that AP activity corresponds with the basic factorof semen examination. Significant statistical differences occurred between thequality of ejaculates which were qualified or disqualified to deep freezing and AI.These results show that AP activity in raw dog semen plasma can be used as amarker for the dog semen qualification for deep freezing and AI with 95%probability of the prognosis of the results.

  15. Kualitas Semen Beku Kuda dalam Pengencer Susu Skim dan Dimitropoulos dengan Dimetilformamida Sebagai Krioprotektan

    OpenAIRE

    I Arifiantini; I. Supriatna; Aminah

    2007-01-01

    Equine semen are far less tolerant in the freezing and thawing process than bull semen. The stallion spermatozoa are known susceptible to cold-shock relating with the content of their fatty acid on the plasma membrane. The extender is one of determining factors in the success of stallion semen cryopreservation, as an energy source and protector the cell from harmfull effect of cold shock. The common cryoprotective agent (CPA) for mammalian spermatozoa was glycerol, but for stallion semen cry...

  16. Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at pre-freeze and post-thawed ram semen.

    Science.gov (United States)

    Sangeeta, Sharon; Arangasamy, A; Kulkarni, S; Selvaraju, S

    2015-10-01

    The possibility of including amino acids for cryopreservation of ram semen to improve the quality of frozen semen was explored in this study in sheep model. 24 samples were collected in triplicate from 8 rams of 2-3 year old Bannur cross bred rams maintained at the Institute Experimental Livestock Unit. Semen was diluted in tris-egg yolk glycerol diluent and made into 7 aliquots as follows: aliquot 1 served as control, "l-alanine" was added at 100 and 135mM in the aliquots 2 and 3, "l-glutamine" was added at 20 and 25mM in the aliquots 4 and 5 and "l-proline" was added at 25 and 50mM in the aliquots 6 and 7, respectively. Diluted semen was filled in 0.25ml French straws and frozen in LN2. Inclusion of "l-proline" and "l-glutamine" in the diluent increased the percent live sperm (Psemen additive to freeze ram semen as they prevented cryoinjuries to sperm and improved the pre-freeze and post-thaw semen characteristics. PMID:26362050

  17. Reproduction in nondomestic birds: Physiology, semen collection, artificial insemination and cryopreservation

    Science.gov (United States)

    Gee, G.F.; Bertschinger, H.; Donoghue, A.M.; Blanco, J.; Soley, J.

    2004-01-01

    Pioneering work by Quinn and Burrows in the late 1930s led to successful artificial insemination (AI) programs in the domestic poultry industry. A variety of species specific modifications to the Quinn and Burrows massage technique made AI possible in nondomestic birds. Massage semen collection and insemination techniques span the entire range of species from sparrows to ostriches. Also, cooperative semen collection and electroejaculation have found limited use in some nondomestic species. Artificial insemination produces good fertility, often exceeding fertility levels in naturally copulating populations. However, aviculturists should explore other ways to improve fertility before resorting to AI. Artificial insemination is labor intensive and may pose risks to nondomestic birds as well as handlers associated with capture and insemination. Semen collection and AI makes semen cryopreservation and germ plasma preservation possible. Yet, semen cryopreservation techniques need improvement before fertility with frozen-thawed semen will equal fertility from AI with fresh semen.

  18. Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen

    Directory of Open Access Journals (Sweden)

    G.V. Aguiar

    2013-02-01

    Full Text Available The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity were analyzed. It was observed a reduction (P<0.05 in normal sperm morphology, fructose, citric acid, P, Mg and total protein concentration during the dry season, which did not affect the motility, vigor, volume and sperm concentration. Phospholipase A2 activity was increased during the dry season (P<0.05. The analysis of the semen cooled at 4C during 48 hours showed reduction in total motility and vigor sperm during the dry season (P<0.05. Based on these results, we conclude that the best period of year for caprine semen cooling is the rainy season.

  19. Effects of sperm concentration at semen collection and storage period of frozen semen on dairy cow conception.

    Science.gov (United States)

    Haugan, T; Gröhn, Y T; Kommisrud, E; Ropstad, E; Reksen, O

    2007-01-01

    The present study was based on data obtained from artificial inseminations (AIs) performed with cryopreserved semen from elite bulls used in the Norwegian breeding program. Semen was diluted to standardize the number of spermatozoa to 18 million per AI dose. The aim of the study was to investigate whether the net sperm concentration at semen collection and the storage period in liquid nitrogen have any effect on probability of conception in dairy cattle. We demonstrated that the natural range of sperm concentration at semen collection within some of the bulls was associated with the probability of conception. However, no primary trend among bulls was found on the effect of sperm concentration at semen collection. This appears to be due to differences among bulls in their response to the dilution ratio of seminal plasma to extender. The effect of storage time was investigated in semen that had been stored between 1000 days and 2400 days in AI straws in liquid nitrogen at the AI center. Our findings showed that use of semen with the longest storage period, i.e. 1951-2400 days, resulted in a more than one percentage point lower probability of conception than semen with a shorter storage period. In conclusion, the net sperm concentration at semen collection, which affects the dilution ratio of seminal plasma to extender, should be considered individually among bulls to achieve optimal reproductive performance. Furthermore, this study gives support to the idea that a measurable degree of damage to the spermatozoa could occur during the preservation time in liquid nitrogen. PMID:16464545

  20. Impact of cryopreservation on bull () semen proteome.

    Science.gov (United States)

    Westfalewicz, B; Dietrich, M A; Ciereszko, A

    2015-11-01

    Cryopreservation of bull spermatozoa is a well-established technique, allowing artificial insemination of cattle on a commercial scale. However, the extent of proteome changes in seminal plasma and spermatozoa during cryopreservation are not yet fully known. The objective of this study was to compare the proteomes of fresh, equilibrated, and cryopreserved bull semen (spermatozoa and seminal plasma) to establish the changes in semen proteins during the cryopreservation process. Semen was collected from 6 mature Holstein Friesian bulls. After sample processing, comparative analysis and identification of proteins was performed using 2-dimensional difference in-gel electrophoresis coupled with matrix-assisted laser desorption/ionization mass spectrometry. Analysis of spermatozoa extracts revealed that 25 identified protein spots, representing 16 proteins, underwent significant ( < 0.05) changes in abundance due to equilibration and cryopreservation. Eighteen protein spots decreased in abundance, 5 protein spots increased in abundance, and 2 protein spots showed different, specific patterns of abundance changes. Analysis of seminal fluid containing seminal plasma showed that 6 identified protein spots, representing 4 proteins, underwent significant ( < 0.05) changes in abundance due to equilibration and cryopreservation. Two protein spots increased in abundance and 4 decreased in abundance. Semen extending and equilibration seems to be responsible for a significant portion of the proteome changes related to cryopreservation technology. Most sperm proteins affected by equilibration and cryopreservation are membrane bound, and loss of those proteins may reduce natural spermatozoa coating. Further research is needed to unravel the mechanisms of the particular protein changes described in this study and establish the relationship between those changes and sperm quality. PMID:26641044

  1. Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas Semen Beku Ayam Hutan Hijau Post Thawing

    Directory of Open Access Journals (Sweden)

    Wayan Bebas

    2015-06-01

    Full Text Available The process of freezing and thawing on semen can lead to physical stress, often called cold shock, and couses the structural and biochemical damage that affecting cell function and ultimately lead to the death of the cell The aim of this study was to know the effect of the addition of various concentrations of dimethylsulfoxide (DMSO as the intracellular cryoprotectant in phosphate yolk diluent on the post thowing quality of the green jungle fowl semen. The study used eight green jungle fowl semens which were collected with massage techniques. Semen was evaluated macroscopically and microscopically. Good quality semen was diluted with phosphate yolk which was added four different concentration of DMSO, namely 4%, 6%, 8%, and 10%. Semen was then filled and sealed in a mini straw (0.25 mL with the concentration of 150.106 cells, and equilibrated at 4oC for 4 hours. The semen freezing was processed using conventional method. Evaluation was performed on post thawing semen. The evaluation of semen quality included the progressive motility and plasma membrane intact. Data were analyzed by analysis of variance. If there were any significant differences, the data were futher analyzed by Duncan test. The results showed that addition of DMSO concentration of 6% has resulted the progressive motility and intact plasma membrane higher significantly (P <0.05 than those of the addition of DMSO concentration 4%, 8%, and 10%.

  2. Semen donors and STD screening.

    OpenAIRE

    Craig, J. M.; Barratt, C L; Kinghorn, G. R.

    1997-01-01

    AIM: The British Andrology Society recommends screening semen donors for sexually transmitted infections to minimise the risk of pathogen transmission to the mother and fetus. The aim was to review recent findings of semen donor screening and, if appropriate, recommend changes to the screening protocol. SUBJECTS: 175 consecutive men attending for STD screening between January 1992 and December 1995 who had been preselected by the Department of Obstetrics and Gynaecology as suitable semen dono...

  3. Semen quality assessments and their significance in reproductive technology.

    Science.gov (United States)

    Kordan, W; Fraser, L; Wysocki, P; Strzezek, R; Lecewicz, M; Mogielnicka-Brzozowska, M; Dziekońska, A; Soliwoda, D; Koziorowska-Gilun, M

    2013-01-01

    Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservation technologies. Semen quality assessment methods include sperm motility evaluations, analyzed with the computer-assisted semen analysis (CASA) system, and plasma membrane integrity evaluations using fluorescent stains, such as Hoechst 33258 (H33258), SYBR-14, propidium iodide (PI), ethidium homodimer (EthD) and 6-carboxyfluorescein diacetate (CFDA), and biochemical tests, such as the measurement of malondialdehyde (MDA) level. This review addresses the significance of specific fluorochromes and ATP measurements for the evaluation of the sperm mitochondrial status. Laboratory methods used for the evaluation of chromatin status, DNA integrity, and apoptotic changes in spermatozoa have been discussed. Special emphasis has been focused on the application of proteomic techniques, such as two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/MS), for the identification of the properties and functions of seminal plasma proteins in order to define their role in the fertilization-related processes. PMID:24597323

  4. Caracterização de proteínas do plasma seminal e sua relação com parâmetros de qualidade do sêmen criopreservado em ovinos Characterization of seminal plasma proteins and its relationship with quality parameters of frozen semen in ram

    Directory of Open Access Journals (Sweden)

    Priscilla Pereira Moura

    2010-05-01

    Full Text Available Os objetivos deste trabalho foram analisar o perfil proteico do plasma seminal ovino e identificar proteínas relacionadas com a congelabilidade do sêmen que possam ser utilizadas como marcadores para essa característica. Foram utilizados os ejaculados de cinco reprodutores, nos quais foram realizadas avaliações espermáticas e dos quais os plasmas seminais obtidos por centrifugação foram submetidos à eletroforese bidimensional em gel de poliacrilamida. Foram identificados 92 spots, considerando todos os animais analisados. A avaliação dos dados obtidos evidenciou variações significativas nos resultados das análises do sêmen dos animais e uma variabilidade no perfil proteico no plasma seminal dos carneiros. As proteínas 03 (7,9kDa; pI 6,35, 23 (13,6kDa; pI 5,01 e 31 (21,4kDa; pI 4,75 se destacaram, por apresentarem maior expressão e relações com as características espermáticas. Sugere-se que mais estudos sejam realizados para verificar se as proteínas 03, 23 e 31 podem ser utilizadas como marcadores da capacidade criopreservadora do sêmen.The objective of this study was to analyze the protein profile of ram seminal plasma and to identify proteins associated with semen freezability, which could be used as marker for predicting this feature. Semen from five rams was used. The sperm analysis was held and the seminal plasma obtained by centrifugation was submitted to two-dimensional electrophoresis using acrylamide gel. Ninety two spots were identified considering the analyzed animals. The results showed a significant variation among sperm analysis of the animals and variability in the protein profile of the seminal plasma of the rams. The proteins 03 (7.9kDa; pI 6.35, 23 (13.6kDa; pI 5.01 e 31 (21.4kDa; pI 4.75 stood out because they showed higher expression and because of its relationship with the sperm characteristics. It is suggested more studies to verify if proteins 03, 23 and 31 could be used as markers of semen freezability.

  5. RELATIONSHIP BETWEEN SOME ENZYMES ACTIVITY, SPERM MORPHOLOGY AND STALLION SEMEN QUALITY

    Directory of Open Access Journals (Sweden)

    Z. PODSTAWSKI

    2013-12-01

    Full Text Available The sperm morphology is one of the factors determining semen quality besidessperm motility and concentration. An important role in this aspect plays someenzymes which are estimated in raw semen plasma. The examination of numerouspopulations of stallions of different breeds and age performed by Kosiniak-Kamyszet al. (2005 showed that significant differences occurred between stallion semenquality concerning both macro- and microscopic examination and some enzymesactivity. It was found that aspartate aminotranspherase (AspAT, lactatedehydrogenase (LDH and alkaline phosphatase (AP activity and total proteinamount (TP in raw seminal plasma decreased when the percent of sperms withcytoplasmatic droplets increased. The increase of these enzymes activity is observedwith the increase of the number of loose heads. These observations showed thatmany examined factors of the semen and semen plasma decided on its quality and onthis reason that these factors need to be applied for seminological diagnosis.

  6. RELATIONSHIP BETWEEN SOME ENZYMES ACTIVITY, SPERM MORPHOLOGY AND STALLION SEMEN QUALITY

    Directory of Open Access Journals (Sweden)

    PODSTAWSKI Z.

    2007-01-01

    Full Text Available The sperm morphology is one of the factors determining semen quality besidessperm motility and concentration. An important role in this aspect plays someenzymes which are estimated in raw semen plasma. The examination of numerouspopulations of stallions of different breeds and age performed by Kosiniak-Kamyszet al. (2005 showed that significant differences occurred between stallion semenquality concerning both macro- and microscopic examination and some enzymesactivity. It was found that aspartate aminotranspherase (AspAT, lactatedehydrogenase (LDH and alkaline phosphatase (AP activity and total proteinamount (TP in raw seminal plasma decreased when the percent of sperms withcytoplasmatic droplets increased. The increase of these enzymes activity is observedwith the increase of the number of loose heads. These observations showed thatmany examined factors of the semen and semen plasma decided on its quality and onthis reason that these factors need to be applied for seminological diagnosis

  7. Influence of chicken native breeds on some physical and biochemical characteristics and short-term storage of semen.

    Science.gov (United States)

    Mohan, J; Singh, R P; Sastry, K V H; Moudgal, R P; Biswas, A; Shit, N

    2011-06-01

    1. The major objective of this study was to examine the influence of 24-h storage of semen at low temperature on semen characteristics and fertilising ability of spermatozoa in two native breeds (Kadaknath-KN, Aseel Peela-AP) and White Leghorn (WL) chicken. 2. Various physical and biochemical properties of freshly ejaculated semen of KN and AP were investigated. Fertility was examined in freshly-ejaculated as well as 24-h-stored (3°C) semen diluted (1:3) with Beltsville Poultry Semen Extender. 3. No significant difference was observed in sperm motility among the different breeds whereas live counts were higher in WL than the native breeds. Body weight, semen volume and sperm concentration were highest in AP, followed by KN and WL. A similar trend was observed in the percentage of dead and morphologically-abnormal spermatozoa. 4. The activity of acid and alkaline phosphatase in seminal plasma were higher in WL than KN, whereas the opposite trend was recorded for glutamic oxaloacetic and pyruvic transaminases. The cholesterol content of semen was highest in AP, followed by KN and WL. Cholesterol was much lower in seminal plasma compared with whole semen but there were no differences between breeds. Mean values of the methylene blue reduction time test were higher in WL than in the native breeds. 5. Fertility and hatchability, using freshly-diluted semen, were poorer in the native breeds than in WL. The pattern of fertility deteriorated further, especially in native fowls, when the birds were inseminated with 24-h-stored semen. 6. In conclusion, variation in physical and biochemical characteristics of semen in native breeds compared to WL correlated with poor fertility after short-term storage of semen. PMID:21732887

  8. Avian artificial insemination and semen preservation

    Science.gov (United States)

    Gee, G.F.

    1983-01-01

    Summary: Artificial insemination is a practical propagation tool that has been successful with a variety of birds. Cooperative, massage, and electroejaculation and modifications of these three basic methods of semen collection are described for a variety of birds. Semen color and consistency and sperm number, moti!ity, and morphology, as discussed, are useful indicators of semen quality, but the most reliable test of semen quality is the production of fertile eggs. Successful cryogenic preservation of avian semen with DMSO or glycerol as the cryoprotectant has been possible. Although the methods for preservation require special equipment, use of frozen semen requires only simple insemination supplies

  9. Cryopreservation of American kestrel semen with dimethylsulfoxide

    Science.gov (United States)

    Gee, G.F.; Morrell, C.A.; Franson, J. Christian; Pattee, Oliver H.

    1993-01-01

    Semen samples from 15 male American Kestrels (Falco sparverius) were frozen in dimethyl sulfoxide (DMSO). The semen was thawed 1-14 mo later and used to inseminate six females during three breeding seasons. Kestrels inseminated with thawed semen containing 4% DMSO produced only infertile eggs (N = 14). Kestrels inseminated with thawed semen containing 6%, 8%, or 10% DMSO produced fertile eggs (N = 14) and live chicks (N = 6). Progressive motility of spermatozoa in thawed semen containing 10% DMSO was less (44 ? 6%) than in thawed semen containing 6% (62 ? 10%) or 8% (61 ? 1%) DMSO.

  10. Effect of dietary parsley (Petroselinium crispum supplementation on semen quality of local Iraqi ganders

    Directory of Open Access Journals (Sweden)

    Hazim J. Al-Daraji,

    2011-08-01

    Full Text Available This study was carried out to investigate the effect of dietary supplementation with different levels of parsley on semen quality of local Iraqi ganders. A total of thirty two local ganders were used in this study during the period from beginning of February to the end of April. The ganders were allocated for 4 treatment groups containing 8 ganders each. Treatment groups were as follows: Control diet (free from parsley, T1: Control diet + 80 g/d parsley, T2: Control diet + 160 g/d parsley; T3: Control diet + 240 g/d parsley. Semen samples were collected twice a week fortnightly from each gander by dorsal-abdominal message method. First semen collection was used to evaluate semen volume, sperm concentration, live in total sperm, live and normal morphology sperm, semen quality factor, sperm motility, abnormal sperm, acrosomal abnormalities, spermatocrit and pH of semen. However, the second semen collection was used for determine seminal plasma concentrations of glucose, protein, cholesterol & activities of aspartate aminotransferase (AST, alanine aminotransferase (ALT and alkaline phosphatase (ALP enzymes. Results revealed that feeding diets containing different levels of parsley (T1, T2, and T3 resulted in significant (P<0.05 increase in semen volume, sperm concentration, live and normal morphology sperm, semen quality factor, sperm motility, spermatocrit and seminal plasma activity of ALP enzyme and significant (P< 0.05 decrease in abnormal sperm and acrosomal abnormalities and seminal plasma concentrations of glucose, protein, and cholesterol and seminal plasma activities of AST and ALT enzymes as compared with control group. There was no significant difference in pH of semen among the control and experimental groups (C, T1, T2, and T3. In conclusion, dietary supplementation with different levels of parsley especially at the level of 240 g/d (T3 caused significant improve- ment with relation to semen traits. So, parsley can be used as an effective tool for improve semen quality of ganders.

  11. Concentration, activity and biochemical characterization of myeloperoxidase in fresh and post-thaw equine semen and their implication on freezability.

    Science.gov (United States)

    Ponthier, J; Franck, T; Parrilla-Hernandez, S; Niesten, A; de la Rebiere, G; Serteyn, D; Deleuze, S

    2014-04-01

    Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western blot. Purified active MPO was added in saline solution and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal-Wallis test, and correlations were determined using Spearman's test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post-thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = -0.5576, p = 0.0086). Inactive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post-thaw loss of activity is partially explained by MPO inactivation in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor. PMID:24479950

  12. 9 CFR 98.34 - Import permits for poultry semen and animal semen.

    Science.gov (United States)

    2010-01-01

    ... section have been met. (d) Sheep and goat semen from regions where scrapie exists. Importation of semen of... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Import permits for poultry semen and animal semen. 98.34 Section 98.34 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION...

  13. Kualitas Semen Beku Kuda dalam Pengencer Susu Skim dan Dimitropoulos dengan Dimetilformamida Sebagai Krioprotektan

    Directory of Open Access Journals (Sweden)

    I. Arifiantini

    2007-08-01

    Full Text Available Equine semen are far less tolerant in the freezing and thawing process than bull semen. The stallion spermatozoa are known susceptible to cold-shock relating with the content of their fatty acid on the plasma membrane. The extender is one of determining factors in the success of stallion semen cryopreservation, as an energy source and protector the cell from harmfull effect of cold shock. The common cryoprotective agent (CPA for mammalian spermatozoa was glycerol, but for stallion semen cryopreservation, dimethyl formamide (DMF was more suitable. This research was conducted to compare the success of the stallion semen cryopreservation in skim milk and dimitropoulos (DV extender with DMF as cryoprotectant. Semen from three sexualy mature stallions was collected twice a week using an artificial vagina. Semen was evaluated macro- and microscopically and then divided into two tubes, diluted each of them with skim milk dan DV extender (1:1, centrifuged at 3 000 rpm for 15 minutes. The supernatant was removed and the pellet (spermatozoa was re-diluted in skim DMF (SDMF and DVDMF extender with the concentration of spermatozoa was 200x106 ml-1. The semen then packed in 0.3ml minitub straw equilibrated for two hours at 4-5oC and frezee in liquid N2 vapor for 10 minutes. The assessment of sperm quality was conducted based on the percentage of sperm motility and viability. In this research, post-thawed semen in DVDMF showed the percentages of the motility (36.2% and the viability (59.3% higher (P<0.05 than SDMF (28.5 and 48.0 %. In conclusion, the DVDMF extender provided better post-thawed semen quality than SDMF.

  14. Palmitoleate enhances quality of rooster semen during chilled storage.

    Science.gov (United States)

    Rad, Hamed Mirzaei; Eslami, Mohsen; Ghanie, Abolfazl

    2016-02-01

    The practice of artificial insemination is widely utilized in poultry; and this requires a broad use of semen storage techniques to prevent the reduction of fertilizing ability of stored semen. The antioxidant activity of palmitoleic acid with in vitro experiments has been shown. The present study was designed to evaluate the effect of palmitoleic acid on the quality of rooster semen stored at 4C. Semen was collected from ten roosters twice a week. Ejaculates with greater than 80% forward spermatozoa motility were pooled and after dilution semen was enriched with 0 (control), 0.125 (P 0.125), 0.25 (P 0.25), 0.5 (P 0.5) and 1 (P 1) millimolar palmitoleate. Forward spermatozoa progressive motility and viability, as well as amounts of malondialdehyde (MDA) and total antioxidant activity (AOA) were evaluated in seminal plasma and spermatozoa at 0, 24 and 48h of storage. Motility was 78.52.21, 77.51.04, and 69.52.32% at 24h and 58.661.35, 49.331.36 and 43.002.08% at 48h in P 0.125, P 0.25 and control, respectively (P<0.02). There were no significant differences in amount of MDA in the seminal plasma among groups, while the amounts of MDA in spermatozoa were less in the P 0.125, P 0.25 and P 0.5 groups compared to the control group at 24 and 48h of storage (P<0.002). Total amounts of AOA in seminal plasma were greater in palmitoleate treatment groups than the control at 24 and 48h (P<0.01). Moreover, palmitoleate treatment groups had greater values of total AOA in spermatozoa compared to the control group at 24 and 48h of storage (P<0.05). In conclusion, enrichment of rooster semen with small doses of palmitoleate has beneficial effects on the semen quality during cold storage. PMID:26723480

  15. Growth, testis size, spermatogenesis, semen parameters and seminal plasma and sperm membrane protein profile during the reproductive development of male goats supplemented with de-oiled castor cake.

    Science.gov (United States)

    Oliveira, C H A; Silva, A M; Silva, L M; van Tilburg, M F; Fernandes, C C L; Velho, A L M C; Moura, A A; Moreno, F B M B; Monteiro-Moreira, A C O; Moreira, R A; Lima, I M T; Rondina, D

    2015-06-01

    The present study was conducted to evaluate the effect of de-oiled castor cake on reproductive traits of crossbreed goats. Fourteen males were grouped into two lots (n = 7/group), as described: group without de-oiled castor cake (WCC) and group fed with de-oiled castor cake (CC). Goats received two diets containing a mixture of Bermudagrass hay and concentrates with the same energy (73% total digestive nutrients) and protein content (15% crude protein) during 150 days, corresponding to ages from 40 (puberty) to 60 weeks. Blood plasma concentrations of urea, albumin, lactate dehydrogenase, creatinine, alanine aminotransferase and testosterone were determined. We also evaluated scrotal circumference, sperm parameters, quantitative aspects of spermatogenesis and daily sperm production (DSP), as well as the proteome of seminal plasma and sperm membrane. Seminal fluid and sperm proteins were analyzed by 2D SDS-PAGE and mass spectrometry. After 150 days of castor cake feeding, animals had no changes in the biochemical composition of blood plasma, suggesting the absence of intoxication by ingestion of ricin. There were no alterations in dry mater intake, weight gain, testis size, peripheral concentrations of testosterone, sperm concentration, motility and morphology. Sertoli and germ cell populations in the testis and DSP were not affected either. However, there were significant variations in the expression of five seminal plasma proteins and four sperm membrane proteins. In conclusion, the replacement of soybean meal by castor cake (with ricin concentrations of 50mg/kg) did not interfere with the growth and core reproductive development of male goats. However, the diet with ricin altered the expression of certain seminal plasma and sperm membrane proteins, which play roles in sperm function and fertilization. Lower expression of these proteins may impair the ricin-fed animals to perform as high-fertility sires. PMID:25883025

  16. [Pharmacokinetic effect of combined administration on spinosin and ferulic acid in monarch drug Ziziphi Spinosae Semen kernel].

    Science.gov (United States)

    Gao, Rong; Li, Shan; Chen, Xian-jin; Wang, Xiao-feng; Wang, Shi-xiang; Fang, Min-feng

    2015-08-01

    To study the pharmacokinetic effect of different combined administration with monarch drug Ziziphi Spinosae Semen on its main components in rats. Sprague-Dawley (SD) rats were randomly divided into Ziziphi Spinosae Semen group, Ziziphi Spinosae Semen-Fructus Schisandrae Chinensis group, Ziziphi spinosae Semen-Salviae Miltiorrhize Radix et Rhizoma group and Zaoren Ansheng prescription group. After oral administration, HPLC was eluted with the mobile phase of acetonitrle-0.03% phosphate acid water in a gradient mode. The detection wavelength was 280 nm. The pharmacokinetic parameters of spinosin and ferulic acid were calculated by DAS 2. 0 software. Compared with Ziziphi Spinosae Semen group, Ziziphi Spinosae Semen-Fructus Schisandrae Chinensis group, Ziziphi Spinosae Semen-Salviae miltiorrhizae Radix et Rhizoma group showed a lower maximum plasma concentration (C(max)) and area under curve (AUC(0-t)) for spinosin and ferulic acid but higher clearance speed (CL/F); whereas the Zaoren Ansheng prescription group showed higher maximum plasma concentration (C(max)) and area under curve (AUC(0-t)) for spinosin and ferulic acid but lower clearance speed (CL/F). Compared with Ziziphi Spinosae Semen group, prescription group showed slower metabolism of spinosin and ferulic PMID:26790310

  17. Microbes in tree swallow semen.

    Science.gov (United States)

    Lombardo, M P; Thorpe, P A

    2000-07-01

    A frequently hypothesized but poorly studied cost of multiple mating in birds is that exposure to pathogenic sexually transmitted microbes (STM's) can lower reproductive success. Conversely, female birds may benefit from high frequencies of copulation and multiple copulation partners if they receive cloacal inoculations of beneficial STM's that can either protect them against future encounters with pathogens and/or serve as therapy against present infection. We examined the semen of 30 male tree swallows (Tachycineta bicolor) in 1998 to determine the presence and prevalence of potential pathogenic and beneficial STM's. Semen was collected directly from males after applying gentle pressure to the cloaca and we used standard microbiological techniques to identify microbes. We found that 19 of 30 samples contained one or more types of microbes. In these 19 positive samples, we isolated both pathogenic and beneficial microbes from 11, only pathogenic microbes from seven, and only beneficial microbes from one. This variation among males suggests that females would benefit from considering a particular male's potential as a donor of either pathogenic or beneficial STM's as a criterion for mate choice. There were few significant differences between males with pathogen-infected semen and those without pathogens in their semen in measures of size, morphology, and ectoparasite score and feather damage. Likewise, there were few significant differences between males with beneficial Lactobacilli spp. in their semen and those without Lactobacilli spp. in their semen in measures of size, morphology, and ectoparasite score and feather damage. We were unable to determine if there was a relationship between microbe presence and prevalence on reproductive performance. PMID:10941730

  18. Morphological Defects in Turkey Semen

    OpenAIRE

    ALKAN, Serhat; BARAN, Alper; ZDA?, . Banu; EVECEN, Mithat

    2002-01-01

    The goal of this study was to evaluate the abnormal spermatozoa rates and types of American Bronze turkeys under Turkish conditions. Semen was collected from toms by abdominal massage and pooled once a week for 10 weeks. After pooling, the semen samples were examined morphologically and mean abnormal rates and types were evaluated. A total abnormal spermatozoa rate of 17 0.06% was determined. Acrosome and mid-piece defects were the leading defects at 66 0.36%. Tail defects of 22 0.18% a...

  19. Use of immobilized cryopreserved bovine semen in a blind artificial insemination trial.

    Science.gov (United States)

    Standerholen, Fride Berg; Waterhouse, Karin Elisabeth; Larsgard, Anne Guro; Garmo, Randi Therese; Myromslien, Frøydis Deinboll; Sunde, Jan; Ropstad, Erik; Klinkenberg, Geir; Kommisrud, Elisabeth

    2015-08-01

    To make timing of artificial insemination (AI) relative to ovulation less critical, methods for prolonging shelf life of spermatozoa in vivo after AI have been attempted to be developed. Encapsulation of sperm cells is a documented technology, and recently, a technology in which sperm cells are embedded in alginate gel has been introduced and commercialized. In this study, standard processed semen with the Biladyl extender (control) was compared with semen processed by sperm immobilization technology developed by SpermVital AS in a blind field trial. Moreover, in vitro acrosome and plasma membrane integrity was assessed and compared with AI fertility data for possible correlation. Semen from 16 Norwegian Red young bulls with unknown fertility was collected and processed after splitting the semen in two aliquots. These aliquots were processed with the standard Biladyl extender or the SpermVital extender to a final number of 12 × 10(6) and 25 × 10(6) spermatozoa/dose, respectively. In total, 2000 semen doses were produced from each bull, divided equally by treatment. Artificial insemination doses were set up to design a blinded AI regime; 5 + 5 straws from each extender within ejaculates in ten-straw goblets were distributed to AI technicians and veterinarians all over Norway. Outcomes of the inseminations were measured as 56-day nonreturn rate (NRR). Postthaw sperm quality was assessed by flow cytometry using propidium iodide and Alexa 488-conjugated peanut agglutinin to assess the proportion of plasma membrane and acrosome-intact sperm cells, respectively. In total, data from 14,125 first inseminations performed over a 12-month period, 7081 with Biladyl and 7044 with SpermVital semen, were used in the statistical analyses. There was no significant difference in 56-day NRR for the two semen categories, overall NRR being 72.5% and 72.7% for Biladyl and SpermVital, respectively. The flow cytometric results revealed a significant higher level of acrosome-intact live spermatozoa in Biladyl-processed semen compared to SpermVital semen. The results indicate that the level of acrosome-intact live spermatozoa in the AI dose did not affect the 56-day NRR for the two semen processing methods. In conclusion, this study has showed that immobilized spermatozoa provide equal fertility results as standard processed semen when AI is performed in a blinded field trial, although the immobilization procedure caused increased sperm damage evaluated in vitro compared to standard semen processing procedure. PMID:25922170

  20. Histamine-2 Receptor Antagonists and Semen Quality.

    Science.gov (United States)

    Banihani, Saleem A

    2016-01-01

    Histamine-2 receptor antagonists are a class of drugs used to treat the acid-related gastrointestinal diseases such as ulcer and gastro-oesophageal reflux disease. Although such drugs, especially ranitidine and famotidine, are still widely used, their effects on semen quality, and hence on male infertility, is still unclear. This MiniReview systematically addresses and summarizes the effect of histamine-2 receptor antagonists (cimetidine, ranitidine, nizatidine and famotidine) on semen quality, particularly, on sperm function. Cimetidine appears to have adverse effects on semen quality. While the effects of ranitidine and nizatidine on semen quality are still controversial, famotidine does not appear to change semen quality. Therefore, additional studies will be required to clarify whether histamine-2 receptor-independent effects of these drugs play a role in semen quality as well as further clinical studies including direct comparison of the histamine-2 receptor antagonists. PMID:26176290

  1. Semen-induced ovulation in the bactrian camel (Camelus bactrianus).

    Science.gov (United States)

    Chen, B X; Yuen, Z X; Pan, G W

    1985-07-01

    Bactrian camels (63 female female, 8 male male) were used in the breeding season to determine the factors that will induce ovulation. After insemination of semen samples into the vagina, the ovaries were checked for ovulation by rectal palpation. The results indicated that ovulation was induced by the seminal plasma, but not by the spermatozoa, and the incidence of ovulation after insemination was 87%. Most of the females (66%) had ovulated by 36 h after insemination and the rest by 48 h, as after natural service. The least amount of semen required to elicit ovulation was about 1.0 ml. Intramuscular injections of LH, hCG and LHRH also caused ovulation, even in females that had not ovulated in response to insemination. PMID:3900379

  2. Semen Allergy Manifesting As Chronic Pruritus Vulva

    Directory of Open Access Journals (Sweden)

    Pavithran K

    1997-01-01

    Full Text Available A young woman of 24 with personal and family history of atopy development pruritus vulva each time after sexual intercourse with her husband. History of urticaria of sites of contact with semen on her thighs gave suspicion of contact urticaria. Positive wheal and flare response to pin prick test with semen, excellent therapeutic response to topical steroid and oral Cetirizine and non- recurrence of the problem after using condom by her husband confirmed the diagnosis of semen allergy.

  3. Therapeutic donor insemination with frozen semen.

    OpenAIRE

    Scott, S G; Mortimer, D.; P J Taylor; Leader, A; Pattinson, H A

    1990-01-01

    Although it is now accepted that cryopreserved semen must, on ethical and medicolegal grounds, be used for donor insemination many clinicians still believe that it has an unacceptably reduced fecundability rate as compared with fresh semen. We studied the outcome of 81 recipients who started therapeutic donor insemination (TDI) treatment during 1986 in a program that used exclusively cryopreserved semen; 55 had never undergone TDI and were receiving the first series (six cycles), 6 were recei...

  4. Effect of centrifugation and sugar supplementation on the semen cryopreservation of captive collared peccaries (Tayassu tajacu).

    Science.gov (United States)

    Castelo, T S; Bezerra, F S B; Lima, G L; Alves, H M; Oliveira, I R S; Santos, E A A; Peixoto, G C X; Silva, A R

    2010-12-01

    The present study is aimed at evaluating the effect of centrifugation for seminal plasma removal and the supplementation of fructose or glucose to the Tris-based extender on the kinematic patterns of the motility parameters of frozen-thawed semen obtained from captive collared peccaries (Tayassu tajacu). Semen samples (n = 14) were collected from 10 sexually mature male collared peccaries by electroejaculation. These samples were further evaluated for parameters such as motility, vigor, sperm viability, membrane integrity, and sperm morphology. The samples were divided into four aliquots, and only two of these aliquots were centrifuged. The semen aliquots (centrifuged and raw semen samples) were diluted in Tris-based extenders supplemented with fructose or glucose. Egg yolk (20%) and glycerol (3%) were added to all the samples which were cryopreserved in liquid nitrogen and thawed at 37 °C/1 min. The frozen-thawed semen was evaluated for the same parameters described for the fresh semen. On the other hand, the kinematic motility patterns were evaluated by a computer-aided system. After thawing, it was observed that the values for the total sperm motility were around 30% for all the samples. A negative effect of centrifugation was verified for parameters such as sperm morphology, linearity, straightness, and beat cross frequency (P 0.05). In conclusion, it is not recommended to centrifuge the ejaculates from collared peccaries prior to conducting the cryopreservative procedures using a Tris-based extender supplemented with fructose or glucose. PMID:20858475

  5. The measurement of reactive oxygen species in human neat semen and in suspended spermatozoa: a comparison

    Directory of Open Access Journals (Sweden)

    Brezinova Jana

    2009-10-01

    Full Text Available Abstract Background It is generally accepted that oxidative stress is an important factor in male infertility because it may impair the physiological function of spermatozoa at the molecular level. Nevertheless, although several approaches have been reported, the imbalance between production of reactive oxygen species (ROS and activity of the antioxidant defense system in semen is difficult to investigate and remains poorly understood. Methods This study compares measurement of ROS production in neat semen and in washed spermatozoa obtained from the same ejaculate, and suspended in phosphate buffered saline using exactly the same luminol-mediated chemiluminescence method. Ninety one samples were obtained from males of infertile couples and 34 from volunteers with proven fertility. Results As expected, ROS levels were markedly lower in neat semen than in washed spermatozoa suspensions where seminal plasma with its potent antioxidant capacity was removed. In the cases of both neat semen and washed spermatozoa, ROS production was lowest in samples from normozoospermic males and highest in samples containing more than half million peroxidase-positive leukocytes per milliliter. For all samples, there was a significant positive correlation between ROS production by neat semen and that by washed spermatozoa suspension. Conclusion Measurement of ROS production in neat semen better reflects actual oxidative status because it detects only the overproduction of ROS which are not effectively scavenged by antioxidant capacity of seminal fluid. The results of our study show a good commutability of both measurements for identification of semen samples with high ROS production. The measurement in neat semen is even less time consuming and therefore easier to implement into laboratory routine.

  6. Assessment of semen quality in Swamp Buffalo AI Bulls in Thailand

    Directory of Open Access Journals (Sweden)

    S. Koonjaenak

    2010-02-01

    Full Text Available Characteristic of Thai swamp buffalo bulls semen used for artificial insemination (AI in Thailand, aspects relevance in freezing and thawing of semen are review. Semen and sperm characteristics were evaluated included sperm count, motility (assessed subjectively and by CASA, morphology (using phase-contrast light microscopy and SEM, plasma membrane integrity (PMI (using a hypo-osmotic swelling test [HOST] and SYBR- 14/propidium iodide [PI], plasma membrane stability (PMS (using Annexin-V/PI and deoxyribonucleic acid (DNA integrity (using SCSA and flow cytometry [FCM]. The average ejaculate volume was about 3.0–4.0 mL, with good viability (PMI measured by the HOST and motility (>65% and >70%, respectively. Sperm concentration ranged from 1.1 to 1.2 billion/mL, being also affected by bull age. Whereas semen quality (including sperm output, pH and initial sperm motility did not differ between the seasons. Few spermatozoa (<15%/ ejaculate had abnormal morphology with abnormalities resembling those in other bovidae. In FT semen, PMI (using SYBR-14/PI and PMS were highest in winter. Across seasons, ~50% of post-thaw spermatozoa depicted linear motility, a proportion that decreased to ~35% during incubation (38oC for 60 minutes, without marking any seasonal difference. The sperm DNA was hardly damaged (with <3% fragmentation, expressed as DNA fragmentation index [DFI], among seasons.

  7. Ebola Virus Persistence in Semen Ex Vivo.

    Science.gov (United States)

    Fischer, Robert J; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J

    2016-02-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions. PMID:26811984

  8. Ebola Virus Persistence in Semen Ex Vivo

    OpenAIRE

    Fischer, Robert J.; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J.

    2016-01-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.

  9. Ebola Virus Persistence in Semen Ex Vivo

    Science.gov (United States)

    Fischer, Robert J.; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent

    2016-01-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions. PMID:26811984

  10. Stainless steel welding and semen quality

    DEFF Research Database (Denmark)

    Jelnes, J E; Knudsen, Lisbeth E.

    Questionnaire studies of patients from fertility clinics suggest that welders may have an increased risk of reduced semen quality. In this study, welders and nonwelders from the same plants were asked to provide blood, urine, and semen samples. Urine was analyzed for chromium and nickel, and for ...

  11. Effect of different antioxidant additives in semen diluent on cryopreservability (−196°C of buffalo semen

    Directory of Open Access Journals (Sweden)

    Hardik A. Patel

    2016-03-01

    Full Text Available Aim: The aim of this study was to evaluate the effect of different antioxidant additives in standard tris-fructose-egg yolkglycerol (TFYG extender on the cryopreservability of buffalo semen. Materials and Methods: Semen collection using artificial vagina, twice weekly for 5 weeks from three pedigreed health breeding bulls of Mehsani breed, aged between 6 and 8 years. Immediately after initial evaluation all 30 qualifying ejaculates (10/bull were split into three aliquots and diluted at 34°C keeping the concentration of 100 million spermatozoa/ml with standard TFYG extender as control and TFYG having two antioxidant additives - Cysteine HCl at 1 mg/ml and ascorbic acid at 0.2 mg/ml to study their comparative performance. Semen filled in French Mini straws using IS-4 system and gradually cooled to 4°C and equilibrated for 4 h in cold handing cabinet. After completion of equilibration, straws were cryopreserved in LN2 by Programmable Bio-freezer. Semen was examined at post-dilution, post-equilibration, and post-thaw stages for sperm quality parameters, and at each stage plasma was separated for enzymatic analysis of aspartate aminotransferase (AST, lactate dehydrogenase (LDH, and alkaline phosphatase (AKP. Results: The mean percentage of sperms in TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at postthaw stage in terms of progressive motility (52.83±0.52, 57.83±0.52, 57.83±0.52, livability (78.70±0.21, 82.33±0.23, 81.73±0.22, and abnormality (5.43±0.21, 5.03±0.17, 5.23±0.18 varied significantly (p<0.05 between control TFYG and TFYG having antioxidant additives. The mean U/L activities of AST (78.70±0.47, 72.80±0.48, 73.30±0.54, LDH (172.70±0.41, 155.78±0.42, 156.33±0.41, and AKP (103.61±0.34, 90.20±0.34, 91.03±0.34 in semen diluted with TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at post-thaw stage, respectively, which showed significantly (p<0.05 higher leakage of enzymes in control TFYG than TFYG incorporated with additives. Conclusion: Incorporation of antioxidant additives such as cysteine HCl and ascorbic acid in standard TFYG diluents improves sperm quality parameters, reduces enzyme leakage, and ultimately advances cryopreservability of buffalo semen.

  12. Leakage of Phosphatases and Fertility of Buck Semen Cryopreserved under Different Freezing Modes

    Directory of Open Access Journals (Sweden)

    Sunanda Sharma

    2014-12-01

    Full Text Available Present study was conducted on 160 ejaculates collected at weekly interval by artificial vagina method from 13 adult Sirohi bucks. Pooled ejaculates were diluted with Tris-egg yolk-citric acid-fructose-glycerol extender (1:4, filled and sealed in straws. Few straws of diluted semen were thawed (40° C/15 seconds and assessed for acid and alkaline phosphatases (ACP and AKP in seminal plasma of diluted semen (Control Group. Remaining semen straws were randomly grouped to constitute freezing mode groups (M1, M2, M2, and M4 and processed further for cryo-preservation of semen. Accordingly diluted semen straws were cooled @-4° C/minute from 25° C up to 5° C thereafter equilibrated for 2 hours and frozen up to -160° C @ 15, 20, 25 and 300 C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were hold at this temperature for 2 minutes then stored separately in LN2. After 7 days of storage, straws from each freezing mode group were thawed and assessed for ACP and AKP in seminal plasma. In vivo fertility trials were also conducted with straws of control (fresh diluted semen as well as freezing mode groups (frozen at different freezing rates. Least square analysis of variance for the data obtained revealed highly significant (P ≤ 0.01 rise in the seminal plasma ACP and AKP enzyme levels in frozen thawed semen as compared to that in fresh diluted semen. The Values of ACP and AKP also differed significantly (P ≤ 0.05 among all the freezing mode groups wherein lowest values of ACP were observed in M3 group followed by M4, M2 and M1 groups in increasing order whereas, lowest values of AKP were observed in M3 followed by M2, M4 and M1 groups in increasing order. Highest fertility rates were observed with semen from M3 followed by M2, M4 and M1 groups. On the basis of enzyme leakage and in-vivo fertility trials, the optimum freezing rate for cryopreservation protocol was arrived at 25° C/minute.

  13. Confidentiality and American semen donors.

    Science.gov (United States)

    Karow, A M

    1993-01-01

    Most American donor insemination programs include a policy of complete confidentiality concerning the donor of the semen. This is the result of a long legal tradition of American constitutional law. However, some slight abridgement of this body of legal decisions might be very much in the best interests of children arising from donor insemination, and even--in most cases, in fact--the donors themselves. With regard to the children, the factors involved are both those of genetic counseling, should the need arise, and psychological development. Of course, as at present, the donor must be relieved of all responsibility, both legal and financial. PMID:8348162

  14. Biophysical and biochemical characteristics of bactrian camel semen collected by artificial vagina.

    Science.gov (United States)

    Mosaferi, S; Niasari-Naslaji, A; Abarghani, A; Gharahdaghi, A A; Gerami, A

    2005-01-01

    Seminal characteristics were investigated in Bactrian camel in this study. Semen samples from ten mature Bactrian camel bulls were collected using a modified bovine artificial vagina. The biophysical parameters including volume, color, sperm concentration and fast forward progressive motility, percentage of live sperm and the biochemical parameters including osmolarity, pH, glucose, calcium, phosphorus, chloride, triglycerides, phospholipids, total protein, albumin and non-protein nitrogen concentrations in seminal plasma were measured. The mean time for semen collection was 5.3 +/- 0.29 min. The volume of semen varies from 1.2 to 26 (8.2 +/- 0.7 mls). The majority of semen samples (83.6%) were milky in color and consistency. The average osmolarity of semen was 316.1 +/- 1.48 mOsm/kg H(2)O. The pH of semen was slightly alkaline (7.4 +/- 0.03). The mean concentration of spermatozoa was 414.8 +/- 25.04 x 10(6)cells/ml. The fast forward progressive motility of spermatozoa was 62.4 +/- 1.57%. The percentage of live spermatozoa was 85.6 +/- 1.15. Seminal plasma concentration of glucose was 35.8 +/- 0.9 mg/dl. Non-protein nitrogen, total protein and albumin were 32.5 +/- 2.5, 2200 +/- 100 and 1100 +/- 100mg/dl, respectively. The average concentrations of phospholipids and triglycerides in seminal plasma were 36.4 +/- 2.1 and 101.6 +/- 5.5mg/dl, respectively. The concentrations of calcium, phosphorus and chloride were 8.2 +/- 0.1, 2.9 +/- 1.7 mg/dl and 97.9 +/- 2.9 mEq./l, respectively. PMID:15589276

  15. Semen collection, cryopreservation and artificial insemination in the dromedary camel.

    Science.gov (United States)

    Deen, Aminu; Vyas, Sumant; Sahani, M S

    2003-07-15

    Collection of semen with a bovine artificial vagina (AV) was attempted with each of 14 camels over a period of 2 years. Semen samples were evaluated, extended and cryopreserved. Frozen thawed semen, diluted cooled semen or whole semen was used to inseminate some female camels which were induced to ovulate with hCG. Males ejaculated semen into the AV in 74.6% collection attempts. The male copulated for at least 200s in 62.9% attempts. The remaining copulations were of shorter duration. Similarly, 49.3% ejaculates were at least 3ml of semen. Libido and donation of semen improved from December onwards and reached a peak after mid January with peak performance persisting until April. It declined during May. The majority of camels had lost libido and refuse to donate semen by the end of May. Camel semen is in gel form. While 35.9% of 203 semen samples exhibited no individual sperm motility, 28.5% exhibited low to fair grade individual sperm motility and only 35.4% exhibited >50% sperm motility. Differences existed between animals (Pcamel semen. Individual sperm motility develops after liquefaction of semen. Addition of caffeine but not chymotrypsin improved the individual motility. The mean live percent sperm count and normal acrosome were 73.3+/-1.0 and 92.0+/-0.5, respectively. Only 51.1% of 45 semen samples with pre-freeze motility of >50% and 25% of 16 semen samples from low pre-freeze motility group with an overall success of 44.2% of 61 semen samples were successfully preserved. Wide variation was observed in the freezability of semen from different males. Attempts to impregnate female camels with liquid semen, frozen thawed semen and whole semen after hCG induced ovulation resulted in 0/10, 1/13 and 4/10 pregnancies. PMID:12695056

  16. Effects of pH during liquid storage of goat semen on sperm viability and fertilizing potential.

    Science.gov (United States)

    Liu, Chang-He; Dong, Hai-Bo; Ma, Dong-Li; Li, You-Wei; Han, Dong; Luo, Ming-Jiu; Chang, Zhong-Le; Tan, Jing-He

    2016-01-01

    A specific problem in goat semen preservation is the detrimental effect of seminal plasma on sperm viability in extenders containing yolk or milk. Thus, the use of chemically defined extenders will have obvious advantages. Although previous studies indicate that the initial pH of an extender is crucial to sustain high sperm motility, changes in extender pH during long-term semen storage have not been observed. Monitoring extender pH at different times of semen storage and modeling its variation according to nonlinear models is thus important for protocol optimization for long-term liquid semen preservation. The present results showed that during long-term liquid storage of goat semen, both sperm motility and semen pH decreased gradually, and a strong correlation was observed between the two. Whereas increasing the initial extender pH from 6.04 to 6.25 or storage with stabilized pH improved, storage with artificially lowered pH impaired sperm motility. Extender renewal improved sperm motility by maintaining a stable pH. Sperm coating with chicken (Gallus gallus) egg yolk improved motility by increasing tolerance to pH decline. A new extender (n-mZAP) with a higher buffering capacity was formulated, and n-mZAP maintained higher sperm motility, membrane integrity and acrosome intactness than the currently used mZAP extender did. Goat semen liquid-stored for 12 d in n-mZAP produced pregnancy and kidding rates similar to those obtained with freshly collected semen following artificial insemination. In conclusion, maintenance of a stable pH during liquid semen storage dramatically improved sperm viability and fertilizing potential. PMID:26612188

  17. Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen Caracrtersticas espermticas e bioqumicas do plasma seminal de caprinos nas estaes chuvosa e seca do Nordeste brasileiro: influncia da estao no resfriamento do smen

    OpenAIRE

    G.V. Aguiar; M F Van Tilburg; A.G.V. Catunda; C.K.S. Celes; I.C.S. Lima; A.C.N. Campos; A.A.A. Moura; A.A. Arajo

    2013-01-01

    The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity) were analyzed. It was observed a reduction (P

  18. Semen analysis under photochemotherapy (PUVA-therapy)

    International Nuclear Information System (INIS)

    In 9 male patients with psoriasis vulgaris a semen analysis before and during photochemotherapy with 8-methoxypsoralen and UVA (PUVA) was performed to rule out drug-induced toxic damage of spermatogenesis or impairment of fertility due to scrotal hyperthermia. Two hours after oral application of 40-60 mg 8-methoxypsoralen the patients had been irradiated in UVA high intensity treatment units. PUVA-treatments were performed four times weekly until total body clearing was achieved. For complete remission 13-26 (mean 20.5) PUVA-treatments were necessary. Corresponding total UVA-doses were 35.3 - 191.0 (mean 83.2) Joule/cm2. The investigated parameters total motility, progressive motility, spermatozoa density, total spermatozoa count, spermatozoa morphology, and seminal plasma fructose remained unchanged. Only the volume of the ejaculate showed a small decrease during 3 months of therapy. From this pilot study there is no evidence, that PUVA-therapy leads to an impairment of fertility in male patients within their reproductive age. (orig.)

  19. Estrs oxidativo en el semen equino criopreservado

    Directory of Open Access Journals (Sweden)

    Giovanni Restrepo Betancur

    2012-01-01

    Full Text Available La escasa fertilidad del semen equino criopreservado ha limitado de manera sustancial su uso en procedimientos de biotecnologa reproductiva. El estrs oxidativo es uno de los factores ms relacionados con este fenmeno, debido al incremento en los niveles de especies reactivas de oxgeno (ERO en el semen como resultado del choque trmico, de la toxicidad de los crioprotectores, y de la alteracin en la disponibilidad y la funcionalidad de los antioxidantes endgenos durante los procesos de criopreservacin. Esta revisin analiza el efecto del estrs oxidativo sobre la fertilidad del semen equino criopreservado.

  20. Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo Addition of seminal plasma to frozen-thawed semen and pregnancy rate of fixed time inseminated ewes

    OpenAIRE

    O.R. Prado; G.M. Bastos; A.L.G. Monteiro; B.B. Saab; S. Gilaverte; C.C. Pierobom; F. Hentz; L.H.S. Martins; C.J.A. Silva; G.S. Dranca; T.S.S. Stivari; G. Cerqueira

    2013-01-01

    Avaliou-se o efeito da adição de plasma seminal ovino ao sêmen descongelado sobre a taxa de prenhez de ovelhas em rebanho comercial. Cento e setenta e quatro ovelhas cruza Texel foram distribuídas em quatro tratamentos: T1) inseminação artificial cervical (IAC) com sêmen descongelado (SD) diluído em solução tampão fosfato salino (PBS); T2) IAC com SD e adição de plasma seminal ovino; T3) grupo-controle I: IAC com sêmen fresco diluído em PBS; T4) grupo-controle II: inseminação artificial por l...

  1. Reduction of concentrate for bovine sires: Influence on metabolic status and semen quality under production conditions

    International Nuclear Information System (INIS)

    The effects of reduced concentrate fed in rations of Holstein Friesian bulls for artificial insemination was evaluated with respect to metabolic status, sexual behaviour, semen production and semen quality during one year. In the first of two studies, twenty bulls were fed diets based on hay, green forage and concentrate according to the standard nutrient requirements for dairy cattle in artificial insemination centres. Bulls were divided into two groups: Group 1 (n = 10, control, 5 kg concentrate) and Group 2 (n = 10, experimental, 1 kg concentrate). Feed, blood semen samples were taken for bromatological analysis, metabolic profile and semen evaluation, respectively. Group 2 had lower plasma concentrations of urea (P<0.001), calcium (P<0.05) and phosphorous (P<0.01). Urea were below the reference range. Season of the year affected lipid metabolite concentrations (P<0.001) and osteotrophic minerals (P<0.05 to P<0.001). Group 2 had better production and quality of semen than did Group 1. In the second study, five bulls were fed as the experimental group in the first study. Time of sampling, season of the year and sire affected the hormonal secretion pattern (P<0.001). There were no differences in testoterone and LH plasma concentrations before and after mounting; however, cortisol concentrations showed a significant raise during the period of maximum excitation. Individual secretion patterns varied between bulls and were related to pathological morphology of reproductive and endocrine organs. The effect of sire was significant on all the indicators of the sperm production, except to percentage of live sperm. Season of the year significantly affected sperm concentration and number of doses of extended sperm produced. It is concluded that a reduction of concentrate in the diet did not affect the metabolic status, sexual behaviour, semen production or sperm quality of sires. 29 refs, 2 figs, 4 tabs

  2. Influence of trace elements and their correlation with semen quality in fertile and infertile subjects

    OpenAIRE

    SUNDARAM, Vickram; SRINIVAS, Muthugadhalli; GURUNATHAN, Jayaraman

    2013-01-01

    There has been increasing curiosity about the appraisal of essential trace elements present in human body fluids and their correlation to human health. Human seminal plasma contains several trace elements that have an imperative role in the normal functioning of the semen. As a result, it is very important to evaluate Zn and other trace elements present in the seminal plasma for the assessment of male infertility. The main objective of this research is to evaluate Zn concentrations and their ...

  3. DISINFECTION BYPRODUCTS AND HUMAN SEMEN QUALITY

    Science.gov (United States)

    This project, also called the Healthy Men Study will examine potential associations between human exposure to drinking water disinfection byproducts, particularly haloacetic acids (HAAs) and trihalomethanes (THMs), and male reproductive health as indicated by semen quality. Sinc...

  4. Artificial insemination of cranes with frozen semen

    Science.gov (United States)

    Gee, G.F.; Sexton, T.J.

    1979-01-01

    For the first time (1978) artificial insemination (AI) with frozen greater sandhill crane (Grus canadensis tabida) semen resulted in fertile eggs and chicks. During the 2 year (1977-78) study, 6 of 27 eggs produced were fertile. Three chicks hatched. Semen samples used for insemination were frozen and stored in liquid nitrogen for two months or less. Recent improvements in the laboratory indicated that a more effective sample can be prepared and greater fertility rates should be expected.

  5. Bovine Leukemia ProVirus: Evidence of Presence of Part of Gag Gene in Seminal Plasma of Naturally Infected Bulls

    Directory of Open Access Journals (Sweden)

    Razi Jafari

    2010-06-01

    Full Text Available It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI. Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen samples from 45 bulls were assessed for the presence of part of gag gene and antibodies to BLV in blood, semen and cell-free fraction of the semen (seminal plasma. Proviral DNA was detected in 5 out of 45 seminal plasma samples. PCR products were sequenced and submitted to gene bank. This data strongly suggested that seminal plasma of seropositive bulls can be positive in PCR.

  6. Study of enzyme activities and protein content of beluga (Huso huso) semen before and after cryopreservation.

    Science.gov (United States)

    Aramli, M S

    2015-02-01

    Knowledge gained regarding the biochemical processes that occur during sperm collection, processing and freezing-thawing might improve current sperm cryopreservation techniques. In our present study, we determined the effect of cryopreservation on the total protein concentration (TP) and the activities of certain enzymes in semen samples from the beluga (Huso huso). The TP content of the seminal plasma of fresh semen was 0.47 0.026 g/l, and the TP after cryopreservation was 1.86 0.6 g/l. The activities of acid phosphatase (0.82 0.042 U/l), lactate dehydrogenase (234.4 19.4 U/l), arylsulfatase (143.1 32.5 U/l) and ?-N-acetylglucosaminidase (58.39 4.14 U/l) in the seminal plasma of fresh semen were significantly lower than those in the supernatant of frozen-thawed semen samples (7.43 0.64, 3224.6 167.2, 422.6 21.3 and 90.2 5.37 U/l respectively). These parameters may be useful as biomarkers for estimating damage to the cell membrane of spermatozoa caused by freezing-thawing. PMID:24780122

  7. Semen abnormalities with SSRI antidepressants.

    Science.gov (United States)

    2015-01-01

    Despite decades of widespread use, the adverse effect profile of "selective" serotonin reuptake inhibitor (SSRI) antidepressants has still not been fully elucidated. Studies in male animals have shown delayed sexual development and reduced fertility. Three prospective cohort studies conducted in over one hundred patients exposed to an SSRI for periods ranging from 5 weeks to 24 months found altered semen param-eters after as little as 3 months of exposure: reduced sperm concentration, reduced sperm motility, a higher percentage of abnormal spermatozoa, and increased levels of sperm DNA fragmentation. One clinical trial showed growth retardation in children considered depressed who were exposed to SSRls. SSRls may have endocrine disrupting properties. Dapoxetine is a short-acting serotonin reuptake inhibitor that is chemically related to fluoxetine and marketed in the European Union for men complaining of premature ejaculation. But the corresponding European summary of product characteristics does not mention any effects on fertility. In practice, based on the data available as of mid-2014, the effects of SSRI exposure on male fertility are unclear. However, it is a risk that should be taken into account and pointed out to male patients who would like to father a child or who are experiencing fertility problems. PMID:25729824

  8. Comparison of four diluents for the retriever dogs semen preservation

    OpenAIRE

    A. Wicaksono; R.I. Arifiantini

    2009-01-01

    The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen w...

  9. Effect of Genotype and Frequency of Semen Collection on Semen Characteristics of Local Chicken Cocks

    Directory of Open Access Journals (Sweden)

    S.N. Ibe

    2006-01-01

    Full Text Available This study reports the effect of genotype and frequency of semen collection on seminal traits of local chicken cocks. Semen was collected, using the back-lumbar massage method from Normal Feather (NOF, Naked Neck (NN, Frizzle (FR and Naked Neck x Frizzle (NNxFR cocks at two ejaculation frequencies, namely once and twice per week for nine weeks. Ejaculates were subjected to both physical and laboratory evaluations for quality. Results showed that there were significant (p<0.05 differences between the genotypes for semen volume with the NOF (0.150.009 mL and NN x FR (0.130.013 mL cocks having higher semen volumes than that of the NN (0.110.013 mL and FR (0.080.013 mL counterparts. Total spermatozoa was the only seminal trait significantly affected by the two frequencies of collection with once a week giving higher values than twice a week collection. Interaction effect was significant for sperm concentration and total spermatozoa. This effect was stronger when semen was harvested twice a week with the NN x FR and NOF cocks producing higher values. It was therefore concluded that NN x FR and NOF genotype were superior to their NN and FR counterparts in both semen output and frequency of semen collections and may be considered as potential candidates for use in natural mating and/or artificial insemination programmes aimed at improving the lot of the local chicken.

  10. 9 CFR 98.36 - Animal semen from Canada.

    Science.gov (United States)

    2010-01-01

    ... There are no importation requirements under this part. (2) Sheep or goat semen The importer or his agent... copies of a declaration; and (iii) A health certificate. (3) Animal semen other than equine, sheep, or... equine, sheep, or goat semen from Canada. If the product is offered for entry at a . . . And . . ....

  11. Characterization and usage of sexed semen from US field data

    Science.gov (United States)

    The objectives were to characterize sexed semen available and its usage from US field data. This included investigating active Holstein proven bulls with sexed semen available, as well as percentages and frequencies of sexed semen matings for heifers and cows. Herds were also characterized for the...

  12. Pengaruh Persentase Bubuk Perlit Terhadap Waktu Ikat Semen

    OpenAIRE

    Sitompul, Lia Kartika

    2014-01-01

    Seiring dengan semakin maraknya perkembangan sektor konstruksi (baik prasarana ataupun sarana) dan sektor properti (pemukiman) maka kebutuhan semen semakin meningkat setiap tahunnya. Meningkatnya kebutuhan semen merupakan salah satu latar belakang permasalahan yang terjadi dalam lingkungan industri konstruksi saat ini. Maka dari itu, perlu dilakukan penelitian lebih jauh lagi tentang material tambah ataupun material pengganti yang dapat digunakan sebagai pengganti semen. Material tambah terse...

  13. Effects of ground semen collection on weight bearing on hindquarters, libido, and semen parameters in stallions.

    Science.gov (United States)

    Burger, D; Meroni, G; Thomas, S; Sieme, H

    2015-09-15

    Collection of semen on the ground from the standing stallion represents an alternative method to dummy mount semen collection and is of increasing popularity for sport stallions, males suffering from health problems, or in studs without a dummy or suitable mare at disposal. Our aim was to collect and compare spermatological and physiological data associated with traditional and ground semen collection. Twelve of 23 Franches-Montagnes stallions were selected to carry out semen collection on a dummy and while standing in a crossed experimental protocol. Semen quantity and quality parameters, weight bearing on hindquarters, and behavioral and libido data were recorded. Ground versus dummy mount semen collection was accompanied by lower seminal volume (15.9 14.6 vs. 22.0 13.3 mL; P < 0.01) and lower total sperm count (4.913 2.721 10(9) vs. 6.544 2.856 10(9) sperm; P < 0.001). No significant differences were found concerning sperm motility and viability. Time to ejaculation was longer, and the number of attempts to ejaculation was higher (P = 0.053) in the standing position compared with the mount on the dummy. A higher (P < 0.01) amount of tail flagging was manifested by the stallions during ejaculation on the dummy compared to when standing. There was no difference in weight bearing on hindquarters when comparing dummy collection (51.2 2.5%) and standing collection (48.9 5.5%). Ground semen collection can be considered as a viable option for stallions that cannot mount a dummy or a mare. However, it requires training and may be not easily accepted by all stallions. Owners should be advised that ground semen collection is associated with significantly lower sperm numbers than with dummy mount semen collection. PMID:26050613

  14. Semen quality in Peruvian pesticide applicators: association between urinary organophosphate metabolites and semen parameters

    Directory of Open Access Journals (Sweden)

    Gasco Manuel

    2008-11-01

    Full Text Available Abstract Background Organophosphates are broad class of chemicals widely used as pesticides throughout the world. We performed a cross-sectional study of associations between dialkylphosphate metabolites of organophosphates and semen quality among pesticide applicators in Majes (Arequipa, Peru. Methods Thirty-one men exposed to organophosphate (OP pesticides and 31 non-exposed were recruited (age, 20–60 years. In exposed subjects, semen and a blood sample were obtained one day after the last pesticide application. Subjects were grouped according to levels of OP metabolites in urine. Semen samples were analyzed for sperm concentration, percentage of sperm motility, percentage of normal morphology, semen leucocytes and concentrations of fructose and zinc. Exposure to OP was assessed by measuring six urinary OP metabolites (dimethyl and diethyl phosphates and thiophosphates by gas chromatography using a single flame photometric detector. Results Diethyldithiophosphate (p = 0.04 and diethylthiophosphate (p = 0.02 better reflected occupational pesticide exposure than other OP metabolites. Semen analysis revealed a significant reduction of semen volume and an increase in semen pH in men with OP metabolites. Multiple regression analysis showed that both occupational exposure to pesticides and the time of exposure to pesticides were more closely related to alterations in semen quality parameters than the single measurement of OP metabolites in urine. Conclusion The study demonstrated that occupational exposure to OP pesticides was more closely related to alterations in semen quality than a single measurement of urine OP metabolites. Current measurement of OP metabolites in urine may not reflect the full risk.

  15. EFFECTS OF SELENOMETHIONINE AND VITAMIN C ON SEMEN.

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    Cristiane A Alvarez e Gentil Vanini de Moraes

    2006-06-01

    Full Text Available Spermatozoa are susceptible to peroxidative damages due to the high concentration of polyunsaturated fatty acids in its cytoplasmic membranes. Studies have shown that the spermatozoa and seminal leukocytes are capable to generate high levels of reactive oxygen species (ROSs that may reduce the viability and fertility of spermatozoa. However, small quantities of ROSs are necessary to initiation of the function of spermatozoa, as well as, capacitation and induction of the acrosomic reaction. Thus an equilibrium between the production of ROSs and antioxidative protection is necessary to assure the spermatic function. The antioxidative protection of the semen is supplied by enzymes such as superoxide dismutase, glutathione peroxidase (GPx, catalase, vitamin C, vitamin E and other substances (albumine, glutathione, taurine, hypotaurine found inside of spermatic cells or in seminal plasma. Thus, the objective of this revision is characterize how reactive oxygen species cause irreparable damages to spermatozoa membranes and the importance of the antioxidative protection of the semen that can be promoted by the addition of simple minerals like selenium and vitamins (e.g. ascorbic acid.

  16. Semen characteristics in HIV-1 positive men and the effect of semen washing.

    Science.gov (United States)

    Lasheeb, A S; King, J; Ball, J K; Curran, R; Barratt, C L; Afnan, M; Pillay, D

    1997-01-01

    We have undertaken an analysis of semen from HIV infected men with regard to sperm counts and motility, non-spermatozoal cells, and viral nucleic acid. Regression analysis showed that sperm concentration and motility were positively associated with blood CD4 cell count. By contrast, non-spermatozoal cell concentration (round cells) was inversely related to CD4 count. Extracellular HIV RNA was detected in the majority of semen samples and proviral DNA in a minority. Percoll gradient washing of 12 semen samples yielded six samples containing adequate sperm concentration for analysis. This washing procedure reduced prewash extracellular RNA to below detectable limits in all cases; proviral DNA present in two of the six prewash samples was also reduced to below detectable limits after washing. We conclude that semen washing before artificial insemination may reduce the risk of HIV transmission from an infected man to an uninfected woman. However, further evidence from prospective analyses of such an approach is required. PMID:9389956

  17. Effects of storage temperature and semen extender on stored canine semen.

    Science.gov (United States)

    Hori, Tatsuya; Yoshikuni, Ryuta; Kobayashi, Masanori; Kawakami, Eiichi

    2014-03-01

    The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference in the qualities of semen diluted with two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender, between the 2, 8 or 12 and the 4°C control groups during storage for up to 48 hr, while the 16-24°C groups showed decreased sperm motility during storage for 48 hr. However, the 2°C group showed slightly lower sperm motility and slightly higher sperm abnormality than the 4°C group. Therefore, we concluded that semen qualities can be maintained for up to 48 hr when canine semen samples are extended with EYT-FC or EYT-GC and stored at a temperature in the range of 4-12°C. PMID:24088408

  18. Correlation of phthalate exposures with semen quality

    International Nuclear Information System (INIS)

    Phthalates are widely used man-made chemical released in the environment and human exposure is mainly through diet. As the phthalate plasticizers are not covalently bound to PVC, they can leach, migrate or evaporate into the environment and as a result have become ubiquitously contaminants. The present study investigates the correlation, if any, between the phthalate esters (DEP, DEHP, DBP, DMP, DOP) and sperm mitochondrial status, ROS, LPO, SCSA, and sperm quality. The study was conducted in the urban/rural population of Lucknow visiting Obstetrics and Gynecology Department, CSMMU, Lucknow. Semen analysis was performed according to the WHO guidelines while phthalate analysis by HPLC and LPO by spectrophotometer and the sperm mitochondrial status, ROS, SCSA using flow cytometry. The questionnaire data showed no significant difference in the demographic characteristics among the groups. In general, urban population was found to have statistically significant higher levels of phthalate esters than the rural. Further, infertile men showed statistically significant (p < 0.05) higher levels of pollutants in the semen than fertile men. A negative correlation between semen phthalate level viz DEHP and sperm quality and positive association with depolarized mitochondria, elevation in ROS production and LPO, DNA fragmentation was established. The findings are suggestive that phthalates might be one among the contributing factors associated with the deterioration in semen quality and these adverse effects might be ROS, LPO and mitochondrial dysfunction mediated

  19. Long-term preservation of chilled canine semen using vitamin C in combination with green tea polyphenol.

    Science.gov (United States)

    Wittayarat, Manita; Kimura, Taichi; Kodama, Risa; Namula, Zhao; Chatdarong, Kaywalee; Techakumphu, Mongkol; Sato, Yoko; Taniguchi, Masayasu; Otoi, Takeshige

    2012-01-01

    Vitamin C and green tea polyphenol are known to have antioxidant effects. The aim of this study was to evaluate the quality of canine semen after preservation with diluents containing vitamin C and polyphenol at 5 degree C for 4 weeks. In experiment 1, we investigated the effects of vitamin C combined with polyphenol supplementation on chilled semen quality. The addition of vitamin C (0.5 or 1 mM) with 0.75 mg per mL polyphenol to semen extender provided significantly higher percentages of sperm motility and viability during cold storage compared to unsupplemented semen. In experiment 2, we determined the optimal working concentration of vitamin C in the semen extender by comparison of a range of concentrations between 0.1 and 20 mM. Supplementation of 0.5 mM vitamin C plus polyphenol yielded the highest percentages of sperm motility and viability; however, there was no beneficial effect on the plasma membrane and acrosomal integrity of the spermatozoa. PMID:22987243

  20. SEMEN QUALITY ASSESSMENT OF NEW ZEALAND WHITE RABBIT BUCKS

    OpenAIRE

    Martyna Błaszczyk; Tomáš Slanina; Peter Massanyi; Robert Stawarz

    2013-01-01

    Rabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30). The semen was obtained by means of artificia...

  1. Communication requested: Boar semen transport through the uterus and possible consequences for insemination.

    Science.gov (United States)

    Rath, D; Knorr, C; Taylor, U

    2016-01-01

    Recent insemination techniques bypass the interactions between sperm and the uterine wall because the semen is deposited deep into the tip of uterine horn or directly into the oviduct. Such techniques allow high dilution of the ejaculates. After normal mating, semen entering the uterus communicates with the uterine milieu. Intact sperm of high mitochondrial membrane potential bind to uterine epithelial cells, whereas most of the unbound sperm in the uterine lumen have damaged membranes. Lectins are the most likely factors to mediate these sperm-uterine interactions. The lectin wheat germ agglutinin is known to induce the strongest binding of sperm, whereas binding is impaired when sialic acid receptors are blocked by wheat germ agglutinin. This suggests that sialic acid is involved in porcine sperm-endometrium interactions, and it is hypothesized that the use of a semen extender supplemented with sialidase would allow insemination with reduced sperm numbers. A lack of contact of sperm and seminal plasma with the uterine wall, as a result of deep insemination, may adversely affect (1) events during ovulation, (2) induction of immunologic tolerance against paternal antigens, (3) preparation of the endometrium for implantation and placentation, and (4) immunologic support required for the fetus during pregnancy. Seminal plasma is known to signal post-insemination changes in the uterine endometrium involving the redistribution of leukocytes. This may involve migration of leukocytes from the uterine wall to the ovary, as seminal plasma particularly increases the appearance of the major histocompatibility complex class II-positive cells. Uterine epithelial cells respond to sperm binding by the production of pro- or anti-inflammatory cytokines. These cytokines may include synchronizing substances, transferred through a counter-current pathway to the ipsilateral ovary, thereby accelerating the final maturation of preovulatory follicles and advancing time of ovulation. In several species, an ovulation-inducing factor exists in seminal plasma, first identified as -nerve growth factor in camelid semen, indicating another pathway that influences the hypothalamic-pituitary-gonadal axis. In summary, low-dose inseminations may not necessarily require semen deposition deep into the uterine horn, as binding inhibitors can circumvent the binding of sperm to the uterine wall. However, subsequent immune-relevant events that control ovulation and prepare the uterine milieu for the developing embryo should be taken into account. PMID:26462662

  2. Monitoring environmental exposures with semen assays

    International Nuclear Information System (INIS)

    Semen studies in humans and animals have yielded extensive and compelling evidence that sperm can be used to assess reproductive potential and diagnose pathology. More recent studies on mutagens and carcinogens both at this and other laboratories suggest that a combination of mouse and human assays can be an efficient, effective approach to monitoring for reproductive hazards in the environment. We are investigating the potential of using variability in sperm morphology and DNA content to quantify and monitor the effects of environmental agents on the human testes. Here we review the status of human and mouse assays for environmental surveillance, discuss the genetic and fertility implications of chemically induced semen changes, and describe the high-speed flow methods being developed to automate sperm assays

  3. Improving outcome of in vitro sperm activation using nonliquefied versus liquefied semen of oligoasthenozoospermic patients

    Directory of Open Access Journals (Sweden)

    Muhammad Fakhrildin

    2010-03-01

    Full Text Available Objective: To evaluate the efficacy of in vitro sperm activation (ISA using non-liquefied versus liquefied asthenozoospermic semen samples for improvement of sperm parameters. Materials and Methods: Fifty six oligoasthenozoospermic (OA patients (age range: 22-44 years; mean: 32.089 years were enrolled in this study. OA patients were classified according to type of infertility. Also, duration of infertility was recorded. Fifty six semen samples were collected, and seminal fluid analyses were done involving macroscopic and microscopic examinations were performed according to WHO methodology. Direct swimup technique was used to separate the motile spermatozoa from seminal plasma. Minimum Essential Medium Eagle (MEME enriched with 5% Human serum albumin (HSA was used. One mL of either nonliquefied or liquefied semen was layered beneath 1 mL of MEME enriched with 5% HSA, and placed for incubation in an air incubator at 37 oC for 30 minutes. Then, one drop (10 ?L from upper layer of culture medium was taken using automatic pipette to be examined under high power field (40 X for assessment of sperm parameters.Results: According to type of infertility, infertile patients were classified into patients with either primary infertility (no. 46; 82.15 % or secondary infertility (no. 10; 17.85 %. In contrast to other parameters, significant (P<0.05 reductions were noticed in the percentages of sperm motility and progressive sperm motility for OA patients with primary infertility as compared to OA patients with secondary infertility. All sperm parameters were significantly (P<0.001 enhanced after in vitro activation of liquefied and non-liquefied semen samples when compared to pre-activation. In the present study, best results were achieved for non-liquefied semen samples as compared to liquefied semen samples.Conclusion: It was concluded that the outcome of ISA was enhanced in regard to sperm parameters when using non-liquefied semen of OA patients. Furthermore, some components of seminal plasma of OA patients may be have harmful effects on certain sperm functions when in vitro incubated for longer periods. Further study is recommended to investigate the effect of in vitro sperm activation from non-liquefied semen on successful rate of artificial insemination husband.

  4. Methods of cryopreservation of Tambaqui semen, Colossoma macropomum.

    Science.gov (United States)

    Varela Junior, A S; Goularte, K L; Alves, J P; Pereira, F A; Silva, E F; Cardoso, T F; Jardim, R D; Streit, D P; Corcini, C D

    2015-06-01

    This study compared three different techniques for sperm cryopreservation of Tambaqui (Colossoma macropomum). Semen was diluted in Beltsville Thawing Solution with the addition of dimethyl sulfoxide (DMSO) at various concentrations (5%, 10%, 15% and 20%). Cryopreservation was performed using three methods: Box Conditioner Method with straws at a 5 cm distance from liquid nitrogen vapor (N2L); Dry Shipper Method placing the straws inside the machine; Vitrification Method placing the straws directly into N2L, amounting to 12 treatments (four DMSO concentrations×three freezing methods). The samples were evaluated for analysis of sperm quality in vivo and in vitro. Use of the Vitrification Method at different concentrations of DMSO provided the least values in the different evaluations. Fertilization, hatching rates and plasma membrane integrity using the Box Conditioner Method with 5% and 10% DMSO did not differ (P>0.05) but use of the concentration of 5% DMSO resulted in greater values than the other treatments (P<0.05) as well as for sperm motility and latency time (P<0.05), although sperm viability was superior using the Dry Shipper Method with 20% of the cryoprotectant. Mitochondrial functionality was impaired by use of the Vitrification Method with all DMSO concentration tested showing the most desirable values when the Box Conditioner Method was used with 5%, 10%, 15% DMSO and the Dry Shipper Method was used with 10% and 15% DMSO. Considering the variables evaluated, the use of the Box Conditioner Method is associated with enhanced Tambaqui semen quality with freeze concentrations of 5% and 10% DMSO. PMID:25906678

  5. Penambahan Osteopontin dalam Pengencer Semen Beku Sapi Perah Friesian Holstein Meningkatkan Ekspresi B-Cell Cll/Lymphoma-2 Spermatozoa Postthawing (ADDITIONAL OSTEOPONTIN INTO FROZEN FRIESIAN-HOLSTEIN SEMEN DILUTER INCREASES THE EXPRESSION OF B-CELL CLL/L

    Directory of Open Access Journals (Sweden)

    Abdul Samik

    2015-05-01

    Full Text Available Post thawed frozen semen viability is one of the most important keys in artificial inseminationdepended on two major cell death mechanism, apoptosis and necrosis. It has been known that good fertilitydiary bulls seminal plasma contains high concentration of osteopontin (OPN. Osteopontin also known ascell survival protein via inhibition to apoptotic cell death, and B-cell CLL/Lymphoma-2 (Bcl-2expressionmostly related to the ability of cell survival. The aim of this study was to investigate the influence ofadditional OPN into frozen semen diluter on post thawed sperm Bcl-2 expression. Fresh semen collectedfrom 4 year Friesian Holstein bull. Treatment group divided into four groups i.e.: control group (P0:without OPN supplementation, (P1: fresh semen with OPN supplementation 5 ?g/5.107 spermatozoa, (P2:fresh semen with OPN supplementation 10 ?g/5.107 spermatozoa, (P3: fresh semen with OPNsupplementation 20 ?g/5.107spermatozoa. Bcl-2 sperm expression was determined usingimunocytochemistry. The result of this study showed that there was no significant difference betweengroup P0 and P1 (p>0,05, but both group P2 and group P3 showed a significant difference with P0 (p<0,05.Nevertheless, there was no significant difference between group P2to group P3 on post thawed FriesianHolstein sperm Bcl-2 expression (p>0,05. The conclusion of this study is osteopontin supplement in frozensemen diluter is capable to increase post thawed Friesian Holstein sperm Bcl-2 expression.

  6. Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen

    OpenAIRE

    O. I. Azawi; M A Ismaeel

    2012-01-01

    The objectives of the present study were to determine the effects of six different antibiotics in controlling the growth of semen contaminating bacteria and if these antibiotics have any adverse effect on Awassi ram spermatozoa. Semen samples from six mature Awassi rams were used in this study. A total number of 120 ejaculates were collected from the rams using an artificial vagina once a week. Semen ejaculates were evaluated for volume, sperm concentration, mass motility, individual motility...

  7. Liquid storage of equine semen: Assessing the effect of d-penicillamine on longevity of ejaculated and epididymal stallion sperm.

    Science.gov (United States)

    Brogan, P T; Beitsma, M; Henning, H; Gadella, B M; Stout, T A E

    2015-08-01

    Short-term storage of equine sperm at 5°C in an extender containing milk and/or egg yolk components is common practice in the equine breeding industry. Sperm motility, viability, DNA integrity and, consequently, fertilizing ability decline over time, partly due to reactive oxygen species (ROS) generation. We investigated whether adding the anti-oxidant d-penicillamine to a commercial milk/egg yolk extender delayed the decrease in semen quality. Semen was recovered on four consecutive days from eight 3-year old Warmblood stallions. On day 5, seven of the stallions were castrated and sperm recovered from the caudae epididymides. Ejaculated samples were split, and one portion was centrifuged and re-suspended to reduce seminal plasma content. All samples were diluted to 50millionsperm/ml and divided into two portions, one of which was supplemented with 0.5mM d-penicillamine. After 48h, 96h, 144h and 192h storage, sperm motility was assessed by computer-assisted semen analysis (CASA), viability by SYBR14/PI staining, and DNA integrity using the sperm chromatin structure assay (SCSA). d-Penicillamine had no effect on motility of ejaculated sperm (P>0.05) but reduced total and progressive motility of epididymal sperm. Sperm chromatin integrity was not influenced by storage time, seminal plasma or d-penicillamine. In short, adding d-penicillamine to a commercial semen extender was neither beneficial nor detrimental to the maintenance of quality in ejaculated semen stored at 5°C. The negative effect on motility of epididymal sperm may reflect differences in (membrane) physiology of spermatozoa that have not been exposed to seminal plasma. PMID:26130601

  8. Environmental exposure to arsenic may reduce human semen quality: associations derived from a Chinese cross-sectional study

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    Xu Weipan

    2012-07-01

    Full Text Available Abstract Background Recent observations in in vitro and in vivo models suggest that arsenic (As is an endocrine disruptor at environmentally-relevant levels. When exposed to As, male rats and mice show steroidogenic dysfunction that can lead to infertility. However, the possible effects of As on human male semen quality remain obscure. Methods We monitored the profile of As species in the urine of a reproductive-age human cohort and assessed its association with semen quality. Men (n = 96 were recruited in an infertility clinic from July 2009 to August 2010 in the Affiliated Hospital of Chongqing Institute for Population and Family Planning. Five urinary As species were analyzed by high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS. Clinical information on the semen volume, sperm concentration and motility was employed to catalogue and evaluate semen quality according to WHO guidelines. As species concentrations in addition to other continuous variables were dichotomized by the medians and modelled as categorical variables in order to explore using the binary logistic regression possible associations between As exposure and semen quality. Results Urinary concentrations (geometric mean ± SD, μg g-1 creatinine of different As species were 7.49 (±24.8 for AsB, 20.9 (±13.7 for DMA, 2.77 (±3.33 for MMA, and 4.03 (±3.67 for Asi (AsiIII and AsiV. DMA concentrations above the median were significantly associated with below-reference sperm concentrations (P =0.02 after adjusting for age, body mass index (BMI, abstinence, smoking and drinking habits. In addition, smoking was positively associated with MMA. Conclusion Reduced parameters in human semen quality are positively associated with As exposure in a reproductive-age Chinese cohort.

  9. Effects of Storage Temperature and Semen Extender on Stored Canine Semen

    OpenAIRE

    Hori, Tatsuya; YOSHIKUNI, Ryuta; KOBAYASHI, MASANORI; Kawakami, Eiichi

    2013-01-01

    ABSTRACT The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference in the qualities of semen diluted with two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender, between the 2, 8 or 12 and the 4°C control groups during storage for up to 48 hr, while the 16–24°C groups showed decreased sperm motility during storage for 48 hr. However, the 2...

  10. Semen collection methods affect the bacterial composition of post-thawed semen of silver barb (Barbodes gonionotus).

    Science.gov (United States)

    Boonthai, Traimat; Khaopong, Weerasith; Sangsong, Jumlong; Sooksawat, Treerat; Nimrat, Subuntith; Vuthiphandchai, Verapong

    2016-03-01

    Biosafety issue associated with the risk of pathogenic contamination of cryopreserved semen is a common concern because of associated declines in sperm quality, storage period and disease transmission. This study was conducted to evaluate the effects of methods of semen collection on sperm quality and bacterial composition of post-thawed semen of silver barb (Barbodes gonionotus). Semen collection methods consisted of four treatments: (1) hand-stripping of abdomen without rinsing of urogenital area with water, (2) hand-stripping of abdomen after rinsing of urogenital area with water, (3) catheterization without rinsing of urogenital area with water and (4) catheterization after rinsing of urogenital area with water. Semen diluted with calcium-free Hank's balanced salt solution containing 10% dimethylsulfoxide (DMSO) was frozen at a freezing rate of -8°Cmin(-1) before plunging in liquid nitrogen. Post-thawed semen collected by catheterization after rinsing urogenital area had the lowest bacterial number, about 2-log reduction of total heterotrophic, Gram negative and pseudomonad bacteria, compared with the other three collection treatments. However, percentages of motile and viable sperm were not significantly (P>0.05) different among treatments. This method eliminated Flavobacterium aquatile, Bacillus megaterium, Kocuria varians, Staphylococcus haemolyticus and Aeromonas media in cryopreserved semen. This is the first report demonstrating the effects of semen collection methods on bacteriological quality of frozen-thawed fish semen. PMID:26778122

  11. Porcine semen as a vector for transmission of viral pathogens.

    Science.gov (United States)

    Maes, Dominiek; Van Soom, Ann; Appeltant, Ruth; Arsenakis, Ioannis; Nauwynck, Hans

    2016-01-01

    Different viruses have been detected in porcine semen. Some of them are on the list of the World Organization for Animal Health (OIE), and consequently, these pathogens are of socioeconomic and/or public health importance and are of major importance in the international trade of animals and animal products. Artificial insemination (AI) is one of the most commonly used assisted reproductive technologies in pig production worldwide. This extensive use has enabled pig producers to benefit from superior genetics at a lower cost compared to natural breeding. However, the broad distribution of processed semen doses for field AI has increased the risk of widespread transmission of swine viral pathogens. Contamination of semen can be due to infections of the boar or can occur during semen collection, processing, and storage. It can result in reduced semen quality, embryonic mortality, endometritis, and systemic infection and/or disease in the recipient female. The presence of viral pathogens in semen can be assessed by demonstration of viable virus, nucleic acid of virus, or indirectly by measuring serum antibodies in the boar. The best way to prevent disease transmission via the semen is to assure that the boars in AI centers are free from the disease, to enforce very strict biosecurity protocols, and to perform routine health monitoring of boars. Prevention of viral semen contamination should be the primary focus because it is easier to prevent contamination than to eliminate viruses once present in semen. Nevertheless, research and development of novel semen processing treatments such as single-layer centrifugation is ongoing and may allow in the future to decontaminate semen. PMID:26506911

  12. Semen phthalate metabolites, semen quality parameters and serum reproductive hormones: A cross-sectional study in China.

    Science.gov (United States)

    Wang, Yi-Xin; Zeng, Qiang; Sun, Yang; Yang, Pan; Wang, Peng; Li, Jin; Huang, Zhen; You, Ling; Huang, Yue-Hui; Wang, Cheng; Li, Yu-Feng; Lu, Wen-Qing

    2016-04-01

    Exposure to phthalates has been found to have adverse effects on male reproductive function in animals. However, the findings from human studies are inconsistent. Here we examined the associations of phthalate exposure with semen quality and reproductive hormones in a Chinese population using phthalate metabolite concentrations measured in semen as biomarkers. Semen (n = 687) and blood samples (n = 342) were collected from the male partners of sub-fertile couples who presented to the Reproductive Center of Tongji Hospital in Wuhan, China. Semen quality parameters and serum reproductive hormone levels were determined. Semen concentrations of 8 phthalate metabolites were assessed using high-performance liquid chromatography and tandem mass spectrometry. Associations of the semen phthalate metabolites with semen quality parameters and serum reproductive hormones were assessed using confounder-adjusted linear and logistic regression models. Semen phthalate metabolites were significantly associated with decreases in semen volume [mono-n-butyl phthalate (MBP), mono-(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP)], sperm curvilinear velocity [monobenzyl phthalate (MBzP), MEHP, the percentage of di-(2-ethylhexyl)-phthalate metabolites excreted as MEHP (%MEHP)], and straight-line velocity (MBzP, MEHP, %MEHP), and also associated with an increased percentage of abnormal heads and tails (MBzP) (all p for trend <0.05). These associations remained suggestive or significant after adjustment for multiple testing. There were no significant associations between semen phthalate metabolites and serum reproductive hormones. Our findings suggest that environmental exposure to phthalates may impair human semen quality. PMID:26766535

  13. Semen evaluation of two selected lines of rabbit bucks.

    OpenAIRE

    Safaa, H.M.; J.S. Vicente; Lavara, R.; Viudes de Castro, M.P.

    2008-01-01

    Twenty rabbit bucks of 9 months of age were used to evaluate semen quality of two lines of New Zealand rabbit bucks selected for litter size at weaning (A line) and growth rate from weaning to slaughter (R line). The morphological semen characteristics indicated that the A line spermatozoa had greater acrosome integrity (+3.6 percentage units; P

  14. Reproductive aspects and storage of semen in camelidae.

    Science.gov (United States)

    Bravo, P W; Skidmore, J A; Zhao, X X

    2000-08-18

    The characteristics of male and female reproductive tracts and reproductive physiology in camelids are described. An account is given on methods of collection, characteristics and storage of semen, and fertility after artificial insemination (AI) with fresh, liquid-stored and frozen-thawed lamoid and camel semen. PMID:10924824

  15. Model Perencanaan Pengangkutan dan Distribusi Semen di Wilayah Indonesia Timur

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    Windra Iswidodo

    2013-03-01

    Full Text Available Pertumbuhan ekonomi dan pembangunan di wilayah Indonesia timur dalam 5 tahun terakhir mengalami peningkatan yang cukup signifikan. Perkembangan infrastruktur di wilayah Indonesia timur merukapak salah satu faktor yang menyebabkan meningkatnya permintaan semen. Akan tetapi peningkatan permintaan semen ini tidak didukung oleh pola distribusi yang baik. Oleh karena itu, diperlukannya suatu solusi untuk memenuhi permintaan semen di wilayah Indonesia timur, salah satunya adalah dengan merencanakan pengangkutan dan distribusi semen dengan jaringan transportasi yang sesuai dengan karekteristik di wilayah Indonesia timur. Tugas akhir ini bertujuan untuk merencanakan rute distribusi semen di wilayah Indonesia timur untuk menghasilkan biaya transportasi yang minimum. Perbandingan antara konsep direct port dengan konsep multiport diharpakan dapat menentukan pola jaringan distribusi semen menuju wilayah Indonesia timur dengan biaya transportasi yang minimum diantara kedua konsep tersebut. Dari hasil pengembangan skenario terdapat titik tujuan yang belum terpenuhi, sehingga rute antisipasi untuk memenuhi permintaan semen pada titik tujuan dengan pola operasi kapal charter. Hasil perhitungan menunjukkan bahwa seluruh titik tujuan distribusi dapat terpenuhi oleh kapal perintis cargo passanger dan kapal charter . Konsep multiport lebih murah dibandingkan direct port, dengan selisih biaya transportasi sebesar 33% untuk contoh rute Ambon-Amahai-Geser-Banda. Sehingga konsep multiport lebih baik diterapakan untuk distribusi semen di wilayah Indonesia timur.

  16. Opportunities to improve liquid and frozen storage of boar semen

    Science.gov (United States)

    Artificial insemination has facilitated the utilization of superior genetics, and has reduced boar biosecurity problems and housing costs. The use of frozen semen permits the flexibility to inseminate animals at unscheduled times and to use semen of deceased boars. While good long-term storage exten...

  17. Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen Caracrtersticas espermticas e bioqumicas do plasma seminal de caprinos nas estaes chuvosa e seca do Nordeste brasileiro: influncia da estao no resfriamento do smen

    Directory of Open Access Journals (Sweden)

    G.V. Aguiar

    2013-02-01

    Full Text Available The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity were analyzed. It was observed a reduction (PVerificou-se as caractersticas seminais de caprinos durante a poca seca e a chuvosa no Nordeste brasileiro e a influncia da poca no resfriamento do smen. Foram mensurados volume, concentrao espermtica, porcentagem de espermatozoides mveis, vigor, morfologia espermtica e caractersticas bioqumicas (frutose, cido ctrico, fsforo, magnsio, protenas totais e atividade da fosfolipase A2. Observou-se reduo (P<0,05 no nmero de espermatozides morfologicamente normais, frutose, cido ctrico, fsforo, magnsio e protenas totais durante a poca seca que no influenciaram na motilidade, vigor, volume e concentrao do smen. Entretanto, a atividade da fosfolipase A2 foi maior na poca seca. Quando o smen foi submetido ao resfriamento a 4C durante 48 horas, houve reduo (P<0,05 na motilidade total e no vigor espermtico durante a poca seca. Com base nesses resultados, conclui-se que o perodo chuvoso melhor para resfriar smen de caprinos no Nordeste brasileiro.

  18. Indian story on semen loss and related Dhat syndrome

    Science.gov (United States)

    Prakash, Om; Kar, Sujit Kumar; Sathyanarayana Rao, T. S.

    2014-01-01

    India is a country of many religions and ancient cultures. Indian culture is largely directed by the Vedic culture since time immemorial. Later Indian culture is influenced by Buddhism, Islam, and Christianity. Indian belief system carries the footprints of these cultures. Every culture describes human behaviors and an interpretation of each human behavior is largely influenced by the core cultural belief system. Sexuality is an important domain which is colored by different cultural colors. Like other cultures, Indian culture believes semen as the precious body fluid which needs to be preserved. Most Indian beliefs consider loss of semen as a threat to the individual. Ancient Indian literature present semen loss as a negative health related event. Dhat syndrome (related to semen loss) is a culture-bound syndrome seen in the natives of Indian subcontinent. This article gathers the Indian concepts related to semen loss. It also outlines belief systems behind problems of Dhat syndrome. PMID:25568479

  19. Effects of Exogenous Glutathione Supplementation in Biocell® Extender on Quality of Cryopreserved Buffalo (Bubalus bubalis Semen

    Directory of Open Access Journals (Sweden)

    Tohid Rezaei Topraggaleah

    2012-01-01

    Full Text Available The study was designed to investigate the effect of exogenous glutathione supplementation in soybean based extender Bioxcell® extender on post thaw semen quality of buffalo (Bubalus bubalis. Split pooled ejaculates (n = 6, possessing >70% visual sperm motility were extended at 37°C with different levels of glutathione (0.0, 0.5, 1 and 2 mM in Bioxcell® extender. Semen was cooled to 4°C in 2 h, equilibrated at 4°C for 4 h, filled in 0.5 mL straws and frozen in a programmable cell freezer before plunging into liquid nitrogen. Thawing of frozen semen was performed after 72 h at 37°C for 30 sec. Sperm motion characteristics, viability, plasma membrane integrity, acrosome morphology of each semen sample immediately after thawing and incubation for 2 h were assessed by using Computer assisted semen analyzer (SCA, eosin-nigrosin staining, Hypo Osmotic Swelling (HOS assay and phase contrast microscope, respectively. Results revealed that the addition 0.5 and 1.0 Mm of glutathione in Bioxcell® extender did not present any significant effect on overall and progressive motility as well as sperm motility characteristics (VAP, VSL, VCL, LIN and ALH, compared to the control groups (p>0.05. Immediately after thawing the proportion of post thaw sperm viability, plasma membrane integrity and normal apical ridge remained similar in all groups. However, glutathione supplementation of the extender with 2.0 mM concentration decreased sperm motility, viability at 0 and 2 h after thawing in a dose dependent manner compared to the control (p0.05. These results revealed that supplementation of the new commercial in soybean based extender Bioxcell® with glutathione did not improve sperm post thaw motility or acrosomal integrity.

  20. Progressive motility - a potential predictive parameter for semen fertilization capacity in bovines.

    Science.gov (United States)

    Li, Y; Kalo, D; Zeron, Y; Roth, Z

    2016-02-01

    We examined the association between progressive motility of spermatozoa and in vitro fertilization (IVF) competence of bovine ejaculates. Fresh semen was evaluated using a computerized sperm quality analyzer for bulls using progressive motility as the primary parameter. Ejaculates with high progressive motility (HPM; >81%) were compared with those with low progressive motility (LPM; 0.05). Examination of sperm morphology revealed a higher proportion of spermatozoa with abnormal morphology (P < 0.01) in LPM versus HPM ejaculates, the predominant abnormal feature being a bent tail (P < 0.05). Sperm viability, acrosome integrity and DNA fragmentation did not differ between HPM and LPM samples. Mitochondrial membrane potential was higher (P < 0.01) in HPM versus LPM semen. Zinc concentrations in the seminal plasma correlated with progressive motility (R2 = 0.463, P = 0.03). In addition, representative ejaculates from HPM and LPM groups were cryopreserved in straws and used for IVF. The proportions of embryos cleaved to 2- and 4-cell stages (88.1 1.1 versus 80.5 1.7, P = 0.001) and developed to blastocysts (33.5 1.6 versus 23.5 2.2, P = 0.026) were higher for HPM than LPM semen. The total cell number of embryos and blastocyst apoptotic index did not differ between groups. Although sperm progressive motility is associated with IVF competence, further examination is required to determine whether progressive motility can serve as a predictor of semen fertilization capacity in vivo. PMID:25532584

  1. Sperm Ubiquitination Correlation with Human Semen Quality

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    MR Sadeghi

    2008-07-01

    Full Text Available Background: Ubiquitin, an 8.5 kDa peptide that marks other proteins for proteasomal degradation, tags defective sperm during epididymal passage. Thus, sperm ubiquitination is a universal marker for sperm defects and can be used as a sperm function test. The objective of the present study was to examine the relationships between sperm ubiquitination and clinical semen parameters, using simplified immunofluorescence assays in order to establish ubiquitin as a biomarker of male infertility. Methods: Semen samples from 100 couples attending Avicenna Infertility Clinic, Tehran, Iran, were collected and analyzed according to WHO criteria. Each sample was washed and adjusted at 106 sperm/ml concentration. Sperm were coated on slides, using cytospin centrifugation and were fixed in buffered formaldehyde. Subsequently ubiquitinated spermatozoa were evaluated by direct immunofluorescence microscopy using FITC-labeled anti-ubiquitin antibodies. After counting at least 200 sperm per sample, while employing light microscopy, the percentage of ubiquitinated spermatozoa was recorded on the same fields through epifluorescence microscopy. Results: Negative correlations were obtained between sperm ubiquitination and sperm count (r=-0.278, P< 0.001, sperm concentration (r=-0.37, P< 0.001, viability (r=-0.407, P< 0.001, sperm morphology (r=-0.509, P< 0.001, rapid progressive motility (a (r=-0.246, P< 0.001 and slow progressive motility (b (r=-0.474, P< 0.001. There was a positive correlation between ubiquitinated sperm and the percentage of immotile spermatozoa (r=0.486, P< 0.000. Conclusion: Increased sperm ubiquitination is inversely associated with good semen quality parameters, supporting the use of ubiquitin as a biomarker for evaluation of human sperm quality.

  2. Testicular cancer and HPV semen infection.

    Science.gov (United States)

    Garolla, Andrea; Pizzol, Damiano; Bertoldo, Alessandro; Ghezzi, Marco; Carraro, Umberto; Ferlin, Alberto; Foresta, Carlo

    2012-01-01

    Testicular cancer represents the more frequent solid tumor affecting males aged 15-35 years. In the last decades, its incidence showed a progressive increased probably due to genetic and environmental factors. Despite exposure to some viruses such as HIV, HCV, EBV, and HPV is frequently related to cancer development, there are no studies aimed to evaluate the possible implication of viral infections in the pathogenesis of testicular cancer. In this study, we analyzed sperm parameters and prevalence of HPV on sperm in 155 testicular cancer patients at diagnosis (T-1), after orchiectomy (T0) and after 12 months from surgery or from the end of adjuvant treatments (T12). All patients showed a significantly higher prevalence of semen infection than controls (9.5% and 2.4% respectively,) and altered sperm parameters both at T-1 and T0. Considering sperm parameters, at T-1 we observed a reduction of progressive motility, and after orchiectomy patients showed a reduction of sperm concentration and count and a further worsening of motility. Thereafter, patients were assigned to three groups on the basis of medical option after surgery: S = surveillance, R = radiotherapy, and C = chemotherapy +/- radiotherapy. At T12, untreated patients had an improvement of sperm parameters while R group and even more C group had a strong decrease of sperm number (p < 0.01 both vs. T0 and S group). Moreover, patients who received radio and/or chemotherapy had a very high prevalence of HPV semen infection (S = 7.7%, R = 30.8%, and C = 61.5%). In conclusion, patients with testicular cancer had frequently altered sperm parameters and higher prevalence of HPV semen infection that were worsened after radio and chemotherapy. Because HPV infection is a risk factor for cancer development and it may further reduce fertility, we suggest screening for HPV in testicular cancer patients at diagnosis and particularly after adjuvant treatments. PMID:23267350

  3. Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis semen

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    S. S. Ramteke

    2014-09-01

    Full Text Available Aim: The experiment was conducted to study the total seminal plasma protein (TSPP and heparin-binding proteins (HBPs in relation to initial semen quality of buffalo bull. Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790 with mass motility of ?3+ were used for the study and categorized into three groups (Group I- Mass motility 3+, Group II- Mass motility 4+ and Group III- Mass motility 5+. Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowrys method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls. Results: The mean values of TSPP concentrations in bull no. 605 and 790 in Group I, II and III were 30.640.12, 31.660.09, 32.530.19 and 28.510.09, 29.490.15, 30.450.17 mg/mL, respectively. The mean values of HBPs concentrations in bull no. 605 and 790 in Group I, II and III were 3.110.07, 3.320.06, 3.460.08 and 2.510.08, 2.910.05, 3.100.03 mg/mL, respectively. Both the values of TSPP and HBPs were significantly higher (p<0.01 in bull no. 605 when compared to 790 in all the three groups. 31 kDa HBP was more intensely present in bull no. 605, thus may indicate its superiority over bull no. 790 in relation to fertility potential. Conclusion: TSPP and HBPs shows variation in concentration with respect to initial semen quality. Furthermore, presence of fertility related 31 kDa HBPs in one of the bull may be an indication of high fertility of a bull. In future, in-vivo and in-vitro correlative study on larger basis is needed for the establishment of fertility-related HBPs in semen which might establish criteria for selection of buffalo bull with high fertility potential.

  4. Semen A Altshuler: scientist, mentor, teacher

    International Nuclear Information System (INIS)

    International Conference 'Resonances in Condensed Matter' is devoted to 100 years of the birthday of the Corresponding member of Academy of Sciences of the USSR, Professor of the Kazan University Semen Alexandrovich Altshuler (1911–1983). He is well known by pioneer works on EPR, the prediction and grounds for an existence of the neutron magnetic moment, the prediction and the theory of the acoustic paramagnetic resonance, and as a founder of the Kazan scientific school 'Magnetic radiospectroscopy of condensed matter' (with E K Zavoiskii and B M Kozyrev)

  5. Reología del semen humano

    OpenAIRE

    Mendeluk, Gabriela R.; Bregni, Carlos; Blanco, Ana M.

    1996-01-01

    El cuadro denominado astenozoospermia posee origen multifactorial y se visualiza como el daño a una de las características funcionales más importantes del espermatozoide humano. la movilidad progresiva lineal rápida (grado a), siendo la consistencia seminal aumentada la condición biofísica de mayor compromiso. En el presente trabajo se estudió la correlación de la consistencia seminal con la viscosidad del semen humano, determinada con los viscosímetros rotacionales Wells- Brookfield y de Ost...

  6. The effect of semen collection method and level of egg yolk on capability of dilution and storage of buck semen

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    N.N. Dhaher

    2013-12-01

    Full Text Available The study was conducted to evaluate the effect of semen collection method for reduction of the deleterious interaction between the enzymes of bulbourethral gland and egg yolk during the dilution and storage of buck semen by three different level of egg yolk. Ten bucks were used in this study; the bucks were divided into two groups (five bucks in each group. Semen samples were collected once a week for four weeks from the bucks in first group using an artificial vagina, and from the animals in second group using an electroejaculator. The collected semen samples were diluted with sodium citrate extender with three different level of egg yolk (5, 10 and 20%. Extend semen samples were stored at 5 C for three days. Computer assisted sperm analysis and Sperm Class Analyzer were used for evaluation of the buck semen samples. Sperm motility parameters were evaluated which includes; percentage of motile sperm, percentage of progressive motile sperm, the value of the linear velocity (VSL, the value of the average velocity (VAP, the value of the curvilinear velocity (VCL, and the amplitude of lateral movement of the head (ALH. Results showed that all sperm motility parameters under the different level of egg yolk in semen samples that collected by artificial vagina were significantly higher than those which collected by electroejaculator. The percentage of motile sperm and progressive motile sperm of samples that collected by artificial vagina were higher at 10% of egg yolk, while these motility parameters were higher at 5% of egg yolk for semen samples that collected by electroejaculator. The differences between the two methods of semen collection in VCL and ALH were clear and the values were higher in samples that collected using the artificial vagina. The values of VSL, VAP and VCL of semen samples that collected by artificial vagina were higher at the second day than first day of semen storage under 10% of egg yolk. In conclusion, there are effects of the semen collection method on ability of dilution and storage of buck semen, and using of artificial vagina and 10% of egg yolk is recommended for buck semen dilution and storage.

  7. Persistence of semen quality during the reproductive period in two strains of turkeys differing in semen yield.

    Science.gov (United States)

    Nestor, K E; Brown, K I

    1976-11-01

    Semen yield, sperm concentration and frequency of bent sperm were measured during the ninth week following stimulatory lighting (in January) and correlated with similar measurements obtained 1-1/2 and 4-1/2 months later in the reproduction period for two strains of medium bodied turkeys differing in semen yield. These correlations, based on measurements made early in the reproductive period, were not large enough so that the male's subsequent performance could accurately be predicted. Strain differences were also apparent in correlation coefficients for semen yield. The correlation coefficients were larger for the strain having the smallest yeild. In general, the significant correlation coefficients between semen yield and sperm concentration were positive and those between semen yield and percent bent sperm and between sperm concentration and percent bent sperm were negative. However, the correlation coefficients tended to be low in magnitude. PMID:1019100

  8. EFFECT OF LOW DENSITY LIPOPROTEINS IN EXTENDER ON FREEZABILITY AND FERTILITY OF EGYPTIAN BUFFALO BULL SEMEN

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    El-Seify

    2012-01-01

    Full Text Available Semen from three Egyptian buffalo bulls was collected once weekly and ejaculates with more 75% progressive motility and more 85 % normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bulls effect. Seven extenders were used: Tris 20 % egg yolk extender with 7 ml glycerol as a control (T1, and substitution of whole egg yolk with 4, 6, 8, 10, 12 and 15 % low density lipoprotein (LDL, T2 – T6, respectively. Semen was diluted to 80 x106 sperm/ml, packaged into 0.25 ml straws, cooled, held at 5.C for 4 h, and then frozen in liquid nitrogen (LN and stored at -196.C for at least one month. Sperm progressive motility, intact acrosome and plasma membrane integrity were assesd at post dilution, equilibration, post-thawing (at 37.C for 30 sec. and after 30 days storage in LN. This study reveled that LDL extenders were more effective in preservation of progressive motility, intact acrosome and integrity of the plasma membrane of buffalo spermatozoa than whole egg yolk extender. Sperm progressive, intact acrosome and plasma membrane integrity were much higher (P < 0.05 in the 12% LDL extender (63.3, 77.17 and 71.3% respectively vs. 35, 40.8 and 34.7% in the control 20% EY extender at post-thawing process, respectively. Fertility rates were higher in extender containing 12% LDLs compared with the control (72.7% vs. 50%, respectively. It was concluded that LDL (12% in extender improved the freezability and fertility of buffalo bull spermatozoa.

  9. Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

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    S. Eskiocak

    2006-05-01

    Full Text Available It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress and 3 months after (non-stress the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10(6/mL, rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63% and seminal plasma arginase activity (0.12 ± 0.01 vs 0.22 ± 0.01 U/mL were significantly lower than in the non-stress situation, whereas seminal plasma NO (17.28 ± 0.56 vs 10.02 ± 0.49 µmol/L was higher compared to the non-stress period (P < 0.001 for all. During stress there was a negative correlation between NO concentration and sperm concentration, the percentage of rapid progressive motility and arginase activity (r = -0.622, P < 0.01; r = -0.425, P < 0.05 and r = -0.445, P < 0.05, respectively. These results indicate that psychological stress causes an increase of NO level and a decrease of arginase activity in the L-arginine-NO pathway. Furthermore, poor sperm quality may be due to excessive production of NO under psychological stress. In the light of these results, we suggest that the arginine-NO pathway, together with arginase and NO synthase, are involved in semen quality under stress conditions.

  10. Cytokines in the blood and semen of infertile patients

    Science.gov (United States)

    Havrylyuk, Anna; Chopyak, Valentyna; Boyko, Yaryna; Kril, Iryna

    2015-01-01

    Cytokines have been important mediators of the immunity and can be involved in numerous processes in the male genital tract including acting as immunomodulatory elements within the male gonad. The aims of this study were: 1) to detect pro- and anti-inflammatory cytokine levels in the control group and subgroups of infertile men; and 2) to set up the practical recommendations concerning determination of cytokine levels for the male infertility diagnosis. Observations were performed in a group of 82 men: healthy controls (n = 27) and infertile patients (n = 55). The male infertility group was further subdivided into patients with: varicocele (n = 22), idiopathic infertility (n = 13) and partners of couples with recurrent spontaneous abortion (RSA; n = 20). Semen analysis was determined following WHO criteria. The cytokine interleukin 1? (IL-1?), IL-6, IL-10, IL-18; tumor necrosis factor ? (TNF-?), interferon g (IFN-g) and transforming growth factor ?1 (TGF-?1) contents in serum and seminal plasma were determined by quantitative ELISA. An interesting marker of male infertility appears to be TGF-?1 (blood) significantly elevated in idiopathically infertile males and in the RSA group. Besides elevated TGF-?1 in a group of idiopathic infertility significantly elevated IL-10, IL-18, IFN-g (blood) and statistically decreased IL-1? while increased IFN-g were revealed in seminal plasma compared to healthy controls. We may postulate novel cytokine micropatterns for patients with different background of infertility. Therefore, circulating cytokines: IL-1?, IL-10, IL-18, TGF-?1, IFN-g and IL-1?, IFN-g and TGF-?1 in seminal plasma should be extended in evaluation of specific types of male infertility. PMID:26648778

  11. Semen cryopreservation protocols of Mangalarga Marchador stallions

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    Marcela Leite Candeias

    2012-09-01

    Full Text Available The effect of the utilization of three semen protocols (Inra 82, Merck Gema and Botu-crio and two filling techniques (0.25 and 0.50 mL straws in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (m/s, straight line velocity (m/s, track velocity (m/s and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.

  12. Environmental Exposure to Triclosan and Semen Quality.

    Science.gov (United States)

    Zhu, Wenting; Zhang, Hao; Tong, Chuanliang; Xie, Chong; Fan, Guohua; Zhao, Shasha; Yu, Xiaogang; Tian, Ying; Zhang, Jun

    2016-01-01

    Triclosan (2,4,4'-trichloro-2'-hydroxy-diphenyl ether, TCS) is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine)(-1) (interquartile range, 0.41-2.95 ng (mg·creatinine)(-1)). A multiple linear regression analysis showed a negative association between natural logarithm (Ln) transformed TCS concentration (Ln-TCS) and Ln transformed number of forward moving sperms (adjusted coefficient β = -0.17; 95% confidence interval (CI) (-0.32, -0.02). Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = -0.35; 95% CI (-0.68, -0.03)), percentage of sperms with normal morphology (β = -1.64; 95% CI (-3.05, -0.23)), as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings. PMID:26901211

  13. Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams

    OpenAIRE

    Rahmat Hidayat; T. Toharmat; A Boediono; I. G. Permana

    2016-01-01

    Contribution of Garut breed sheep as protein resources is not optimal yet.  Acceleration of population growth such as increasing of female offspring is neccesary.  This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K) – (Cl+S)/100 g of dry matter]) and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows:   RN0= basal ration (PKAR +14) withou...

  14. Comparison of four diluents for the retriever dogs semen preservation

    Directory of Open Access Journals (Sweden)

    A. Wicaksono

    2009-03-01

    Full Text Available The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen was evaluated macro-and microscopically. The semen with >70% sperm motility was divided into four tubes and diluted with diluter 1 (P1, diluter: P2, P3 and P4 (modified P3. The diluted semen was divided into two tubes and each sample was stored at room and 50C temperature. The viability of chilled semen was observed every 3 hours at room temperature and 12 hours at 50C. The result showed that P2 keep the sperm viability better than the other diluents. On 50C at 24 hours storage P2 showed the highest motile and live sperm percentage (46.25 ± 0.22%; 57.11 ± 0.25%. In room temperature at 6 hours P2 showed the highest motile and live sperm percentage (40.94 ± 0.20%; 52.65 ± 0.23%. It is concluded that P2 can keep the sperm viability by 84 hours of 50C and 21 hours at room temperature.

  15. Relationships between rabbit semen characteristics and fertilising ability after insemination.

    Science.gov (United States)

    Theau-Clment, M; Ailloud, E; Sanchez, A; Saleil, G; Brun, J M

    2016-03-01

    This study aimed to analyse the relationship between rabbit semen characteristics and semen fertilising ability after insemination, which is generally found to be weak. Our hypothesis was that using high semen dilutions (1 : 19), non-oestrus-stimulated does, and homospermic inseminations would make it easier to predict semen fertilising ability. Semen characteristics were evaluated on 275 ejaculates of 128 INRA1001 bucks, distributed into five successive batches. A total of 1970 inseminations were performed. The continuous semen variables were subdivided into three classes of similar size to account for any non-linear relationship between semen characteristics and fertilising ability. Mass motility was divided into two classes according to the presence or absence of waves under microscope observation. Libido, the presence or absence of gel, volume, percentage of progressive sperms, curvilinear velocity, beat frequency of the flagellum, and straightness and linearity of sperm movement did not affect fertility, prolificacy or productivity. It was confirmed that mass motility, estimated by visual observation under the microscope, significantly influenced fertility as well as the percentage of motile and of rapid sperms, and the amplitude of lateral head displacement, estimated by a computer-assisted semen analysis system. To a lesser extent, the percentage of motile cells and of rapid cells significantly influenced prolificacy. Consequently, mass motility and the percentage of motile cells significantly influenced rabbit doe productivity (+1 live births/AI when the semen showed at least a beginning of wave movement, or when the percentage of motile cells was >84%). Interestingly, a gain of 1.5 rabbits was observed when the percentage of rapid cells changed from 64% to 79%, whereas productivity significantly dropped beyond 83% of rapid cells, reflecting a non-linear relationship. PMID:26549861

  16. Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE

    Directory of Open Access Journals (Sweden)

    Oriza Savitri Ariantie

    2014-05-01

    Full Text Available The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY and Tris soya (TS diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C. The motility,viability and plasma membrane integrity (PMI of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05. The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41% and control(51,78±4,86% which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54% and control (51,11±4,86% which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose  rather than trehalose.

  17. Evaluation of sperm chromatin structure in boar semen

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    Banaszewska Dorota

    2015-06-01

    Full Text Available This study was an attempt to evaluate sperm chromatin structure in the semen of insemination boars. Preparations of semen were stained with acridine orange, aniline blue, and chromomycin A3. Abnormal protamination occurred more frequently in young individuals whose sexual development was not yet complete, but may also be an individual trait. This possibility is important to factor into the decision regarding further exploitation of insemination boars. Thus a precise assessment of abnormalities in the protamination process would seem to be expedient as a tool supplementing morphological and molecular evaluation of semen. Disruptions in nucleoprotein structure can be treated as indicators of the biological value of sperm cells.

  18. Should single layer centrifugation of dog semen be done before or after the semen is cooled?

    Science.gov (United States)

    Gálvez, M J; Ortiz, I; Hidalgo, M; Morrell, J M; Dorado, J

    2015-04-01

    The aim of this study was to assess the effectiveness of sperm selection by single layer centrifugation (SLC) on canine sperm quality when SLC was performed before or after the cooling process, or when double SLC (before and after cooling) was performed. Twenty ejaculates from four dogs were divided into four aliquots as follows: unselected: no SLC was performed; SLC prior to cooling (SLC-PC): sperm selection was carried out before cooling; SLC after cooling (SLC-AC): sperm selection was performed after cooling; and double SLC: sperm selection was carried out before and after cooling. Sperm motility (by computer-assisted semen analysis), morphology (Diff-Quick staining), sperm membrane integrity (Vital-Test kit) and acrosome integrity (double fluorescent stain) were assessed in re-warmed semen samples. Four sperm subpopulations (sP) were detected using a pattern analysis technique (sP1: highly active, non-progressive; sP2: low velocity, highly progressive; sP3: less vigorous, poorly progressive; sP4: highly progressive motility). A higher proportion of sperm were classified as sP4 in SLC-AC samples. Most of the sperm parameters assessed showed higher values in the SLC-AC group. We conclude that SLC-AC is the best protocol to improve sperm quality in chilled canine semen in comparison to the other procedures tested. PMID:25653394

  19. Variations of protein profiles and calcium and phospholipase A2 concentrations in thawed bovine semen and their relation to acrosome reaction

    OpenAIRE

    V. Alonso Marques; L.R. Goulart; A.E.D. Feliciano Silva

    2000-01-01

    Just as calcium plays an integral role in acrosome capacitation and reaction, several spermatozoon proteins have been reported as binding to the ovum at fertilization. We examined the relationship between thawed bovine semen protein profiles, seminal plasma calcium ion concentration, spermatozoon phospholipase A2 (PLA2) activity and acrosome reaction. Electrophoretic profile analysis of spermatozoa and bovine seminal plasma proteins (total and membrane) revealed qualitative and quantitative d...

  20. Do perfluoroalkyl compounds impair human semen quality?

    DEFF Research Database (Denmark)

    Joensen, Ulla Nordström; Bossi, Rossana; Leffers, Henrik; Jensen, Allan Astrup; Skakkebaek, Niels E; Jørgensen, Niels

    2009-01-01

    in 105 Danish men from the general population (median age, 19 years). RESULTS: Considerable levels of perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), and perfluorohexane sulfonic acid were found in all young men (medians of 24.5, 4.9, and 6.6 ng/mL, respectively). Men with high...... combined levels of PFOS and PFOA had a median of 6.2 million normal spermatozoa in their ejaculate in contrast to 15.5 million among men with low PFOS-PFOA (p = 0.030). In addition, we found nonsignificant trends with regard to lower sperm concentration, lower total sperm counts, and altered pituitary......-gonadal hormones among men with high PFOS-PFOA levels. CONCLUSION: High PFAA levels were associated with fewer normal sperm. Thus, high levels of PFAAs may contribute to the otherwise unexplained low semen quality often seen in young men. However, our findings need to be corroborated in larger studies....

  1. Evidence of excretion of Schmallenberg virus in bull semen.

    Science.gov (United States)

    Ponsart, Claire; Pozzi, Nathalie; Bréard, Emmanuel; Catinot, Virginie; Viard, Guillaume; Sailleau, Corinne; Viarouge, Cyril; Gouzil, Julie; Beer, Martin; Zientara, Stéphan; Vitour, Damien

    2014-01-01

    Schmallenberg virus (SBV) is a novel orthobunyavirus, discovered in Germany in late 2011. It mainly infects cattle, sheep and goats and could lead to congenital infection, causing abortion and fetal abnormalities. SBV is transmitted by biting midges from the Culicoides genus and there is no evidence that natural infection occurs directly between ruminants. Here, we could detect SBV RNA in infected bull semen using qRT-PCR (three bulls out of seven tested positive; 29 positive semen batches out of 136). We also found that highly positive semen batches from SBV infected bulls can provoke an acute infection in IFNAR-/- mice, suggesting the potential presence of infectious virus in the semen of SBV infected bulls. PMID:24708245

  2. Semen analysis in the Usher syndrome type 2A.

    Science.gov (United States)

    van Aarem, A; Wagenaar, M; Tonnaer, E; Pieke Dahl, S; Bisseling, J; Janssen, H; Bastiaans, B; Kimberling, W; Cremers, C

    1999-01-01

    Semen analysis in patients with Usher syndrome suggested that defective connecting cilia axonemes may be involved in the irreversible, progressive loss of photoreceptors in Usher's syndrome. In the framework of clinical genetic research into Usher syndrome, a pilot study was set up to test these findings. The semen of 6 Usher 2A patients was analysed. The fertility status of the study group of Usher 2A patients was evaluated, including semen analysis, supplemented by electron microscopic examination of the spermatozoa. Except for a significantly increased pH value, no abnormalities were found in the functional semen analysis, whereas electron microscopy revealed microtubular tail abnormalities. The latter finding was of little relevance, however, in view of the normal motility of the spermatozoa observed in these patients. There were no fertility problems in our group of Usher 2A patients, nor have any been mentioned in Usher patients in general. Earlier study findings were not supported by our data. PMID:10325550

  3. Environmental exposure to lead induces oxidative stress and modulates the function of the antioxidant defense system and the immune system in the semen of males with normal semen profile

    Energy Technology Data Exchange (ETDEWEB)

    Kasperczyk, Aleksandra; Dobrakowski, Michał [Dept. of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, Katowice, Jordana 19, 41-808 Zabrze (Poland); Czuba, Zenon P. [Dept. of Microbiology and Immunology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Katowice, Jordana 19, 41-808 Zabrze (Poland); Horak, Stanisław [I-st Chair and Clin. Dept. of Gynecology, Obstetrics and Gynecological Oncology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Katowice, Batorego 15, 41-902 Bytom (Poland); Kasperczyk, Sławomir, E-mail: kaslav@mp.pl [Dept. of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, Katowice, Jordana 19, 41-808 Zabrze (Poland)

    2015-05-01

    We investigated the associations between environmental exposure to lead and a repertoire of cytokines in seminal plasma of males with normal semen profile according to the WHO criteria. Based on the median lead concentration in seminal plasma, 65 samples were divided into two groups: low (LE) and high exposure to lead (HE). Differences in semen volume and the pH, count, motility and morphology of sperm cells were not observed between the examined groups. The total oxidant status value and the level of protein sulfhydryl groups as well as the activities of manganese superoxide dismutase and catalase were significantly higher in the HE group, whereas the total antioxidant capacity value and the activities of glutathione reductase and glutathione-S-transferase were depressed. IL-7, IL-10, IL-12, and TNF-α levels were significantly higher in the HE group compared with the LE group. Environmental exposure to lead is sufficient to induce oxidative stress in seminal plasma and to modulate antioxidant defense system. - Highlights: • Lead induces oxidative stress in seminal plasma in human. • Lead modulates antioxidant defense system in seminal plasma in human. • Lead does not change a Th1/Th2 imbalance in seminal plasma in human.

  4. Environmental exposure to lead induces oxidative stress and modulates the function of the antioxidant defense system and the immune system in the semen of males with normal semen profile

    International Nuclear Information System (INIS)

    We investigated the associations between environmental exposure to lead and a repertoire of cytokines in seminal plasma of males with normal semen profile according to the WHO criteria. Based on the median lead concentration in seminal plasma, 65 samples were divided into two groups: low (LE) and high exposure to lead (HE). Differences in semen volume and the pH, count, motility and morphology of sperm cells were not observed between the examined groups. The total oxidant status value and the level of protein sulfhydryl groups as well as the activities of manganese superoxide dismutase and catalase were significantly higher in the HE group, whereas the total antioxidant capacity value and the activities of glutathione reductase and glutathione-S-transferase were depressed. IL-7, IL-10, IL-12, and TNF-α levels were significantly higher in the HE group compared with the LE group. Environmental exposure to lead is sufficient to induce oxidative stress in seminal plasma and to modulate antioxidant defense system. - Highlights: • Lead induces oxidative stress in seminal plasma in human. • Lead modulates antioxidant defense system in seminal plasma in human. • Lead does not change a Th1/Th2 imbalance in seminal plasma in human

  5. Environmental Exposure to Triclosan and Semen Quality

    Directory of Open Access Journals (Sweden)

    Wenting Zhu

    2016-02-01

    Full Text Available Triclosan (2,4,4′-trichloro-2′-hydroxy-diphenyl ether, TCS is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine−1 (interquartile range, 0.41–2.95 ng (mg·creatinine−1. A multiple linear regression analysis showed a negative association between natural logarithm (Ln transformed TCS concentration (Ln-TCS and Ln transformed number of forward moving sperms (adjusted coefficient β = −0.17; 95% confidence interval (CI (−0.32, −0.02. Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = −0.35; 95% CI (−0.68, −0.03, percentage of sperms with normal morphology (β = −1.64; 95% CI (−3.05, −0.23, as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings.

  6. SPONTANEOUS POTENTIAL INVESTIGATIONS IN SEMENIC MOUNTAINS

    Directory of Open Access Journals (Sweden)

    P. URDEA

    2014-11-01

    Full Text Available Spontaneous Potential Investigations in Semenic Mountains. The use of geophysical methods such as that of Spontaneous Potential (SP to investigate areas where the geomorphological processes occur, has the role to identify less visible processes as for example subcutaneous erosion or piping, subsoil water drainage and finding specific spatial differences of these processes. Comparative study of these sites allows correlation between geomorphological factors, soil and climate, but also to observe the evolution of subsurface erosion or underground water infiltration over time. During this investigation a series of mesh grids have been made in areas with different characteristics (lithology, pedology, slope, exposition, etc. at different time periods in order to spot and analyse the change in data in the chosen sites, various conditions given. Values expressed in millivolts (mV obtained by the Spontaneous Potential method have been put into an algorithm for interpolation looking to yield a pattern of values of what is happening in the soil during that period of time. Thus, in the autumn, the investigation site at the nivation niche Baia Vulturilor, returned values of between -22.6 mV and 65.6 mV, while in spring in the same site, values were within the range of -14.4 mV / 30.1 mV. On the other hand, on the site of the cryopediment under the Semenic peak, in the spring, return values ranged from -40.4 mV and -1.1 mV. A particular case is that of the glacis near Piatra Goznei peak; in this area anthropogenic electricity influences on soil can be found. Based on some models a trend of water movement in the soil could be established, this depending heavily on the amount of precipitation infiltration, local lithology, depth of soil and their structure, and evapotranspiration process. Water movement in the soil may be a correlation with sediment movement in soil horizons and instability manifested on the slopes.

  7. Environmental Exposure to Triclosan and Semen Quality

    Science.gov (United States)

    Zhu, Wenting; Zhang, Hao; Tong, Chuanliang; Xie, Chong; Fan, Guohua; Zhao, Shasha; Yu, Xiaogang; Tian, Ying; Zhang, Jun

    2016-01-01

    Triclosan (2,4,4′-trichloro-2′-hydroxy-diphenyl ether, TCS) is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine)−1 (interquartile range, 0.41–2.95 ng (mg·creatinine)−1). A multiple linear regression analysis showed a negative association between natural logarithm (Ln) transformed TCS concentration (Ln-TCS) and Ln transformed number of forward moving sperms (adjusted coefficient β = −0.17; 95% confidence interval (CI) (−0.32, −0.02). Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = −0.35; 95% CI (−0.68, −0.03)), percentage of sperms with normal morphology (β = −1.64; 95% CI (−3.05, −0.23)), as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings. PMID:26901211

  8. Efektifitas Penambahan Flour Pada Bahan Restorasi Semen Ionomer Kaca

    OpenAIRE

    Dewi Sri Kesumawati

    2008-01-01

    Semen ionomer kaca adalah bahan tumpatan yang dalam penggunaannya dapat mempertahankan atau menggantikan jaringan gigi sebanyak mungkin, membantu terjadinya proses remineralisasi, dan mempunyai estetik yang baik. Bahan restorasi yang biasa digunakan dan dapat melepaskan fluor adalah semen ionomer kaca. Bahan ini sebagai perekat yang mengikat struktur gigi dan melepas fluor sebanyak yang dibutuhkan gigi agar dapat mencegah terjadinya karies. Dentin merupakan lapisan dibawah email, sehingga...

  9. Wasting semen: context and condom use among the Maasai

    OpenAIRE

    Coast, Ernestina

    2007-01-01

    Motivations for condom use are intricate and the behaviour of individuals and couples takes place in complex sociocultural settings. This study examines in detail the sociocultural context of condom use among the Maasai, an east African agropastoralist population. A review of the ethno-demographic literature demonstrates the sociocultural significance of semen in a range of settings. A detailed description of Maasai values relating to semen is followed by an analysis presenting results from a...

  10. Artificial insemination and cryopreservation of semen from nondomestic birds

    Science.gov (United States)

    Gee, G.F.

    1995-01-01

    Studies of Al and cryopreservation of semen from nondomestic birds began because of the increased emphasis on conservation of avian species threatened with extinction. Over the years, aviculturists have developed techniques for Al and cryopreservation of semen obtained from a variety of birds ranging from passerines to Andean condors. Generally, for each new species, we develop a practical semen collection technique and then evaluate the semen. A commercial semen extender (Beltsville Poultry Semen Extender) is modified and used to dilute the semen and provide support for the sperm during the freezing process (the pH and osmolality of the extender is adjusted to reflect the pH and osmolality of the semen being frozen). We find that the freezing schedule developed by Sexton (1977), which utilizes dimethylsulfoxide (DMS0) as cryoprotectant, works well for many species. We cool the sample sequentially in an ethanol bath, in liquid nitrogen vapor, and lastly in liquid nitrogen. Although we have experimented with a variety of freezing protocols, we prefer a 15-min equilibration period in DMSO at 5 C. We begin the freezing process by cooling at -1 C/min from 5 to -20 C in the ethanol bath. The samples are transferred into a vapor tank at a location just above liquid nitrogen and frozen at -50 C/min to -80 C. To complete the freezing process, the samples are plunged into the liquid nitrogen in the bottom of the vapor tank. The samples remain in liquid nitrogen until they are thawed just before insemination. If necessary, the freezing equipment can be transported in a van to remote locations.

  11. Evidence of excretion of Schmallenberg virus in bull semen

    OpenAIRE

    Ponsart, Claire; Pozzi, Nathalie; Bréard, Emmanuel; Catinot , Virginie; Viard, Guillaume; Sailleau, Corinne; Viarouge, Cyril; Gouzil, Julie; Beer, Martin; Zientara, Stephan; Vitour, Damien

    2014-01-01

    Schmallenberg virus (SBV) is a novel orthobunyavirus, discovered in Germany in late 2011. It mainly infects cattle, sheep and goats and could lead to congenital infection, causing abortion and fetal abnormalities. SBV is transmitted by biting midges from the Culicoides genus and there is no evidence that natural infection occurs directly between ruminants. Here, we could detect SBV RNA in infected bull semen using qRT-PCR (three bulls out of seven tested positive; 29 positive semen batches ou...

  12. Bull Semen Collection and Analysis for Artificial Insemination

    OpenAIRE

    Karolina Barszcz; Dariusz Wiesetek; Michal Wasowicz; Marta Kupczynska

    2012-01-01

    Insemination is acknowledged as a breeding method that contributes to improvement of farm animal populations, particularly of cattle. Artificial insemination allows for maximum use of the most valuable breeders and, at the same time, for significant increase of breeding advance. Moreover, using semen of proved quality reduces the spread of sexually transmitted diseases. The purpose of this study was to present the process of collection and analysis of bulls semen in the Mazovian Centre of An...

  13. Genetic parameters of rabbit semen traits and male fertilising ability.

    Science.gov (United States)

    Brun, J M; Sanchez, A; Ailloud, E; Saleil, G; Theau-Clment, M

    2016-03-01

    This study aimed to estimate genetic parameters for rabbit semen production, semen characteristics and fertilising ability following artificial insemination. It involved five successive batches of 30-36 bucks each, 22 weeks of semen collection, and 11 weeks of semen recording per batch. Semen analyses were based on 2312 ejaculates. A total of 2019 inseminations were performed on 674 females with semen from 236 ejaculates from 128 bucks. Heritability estimates of semen traits ranged from 0.05 to 0.18. At approximately 0.05-0.06 for pH, volume and mass motility, they were higher for concentration (0.10) and the total number of sperms per ejaculate (0.12), and even higher for motility traits based on computer-assisted semen analysis. The percentage of motile sperms had the highest heritability (0.18) and appeared to be a good candidate criterion to select for both sperm number and motility. The heritability estimates were close to zero for all three criteria of fertilising ability: fertility (F), prolificacy (live births, LB) and their product (LB per insemination). A permanent environmental effect of the male seemed to be higher for LB (0.04) than for F (0.01). The rabbit does accounted for approximately 10% of the variance of the three criteria. With respect to the female, the male contribution was negligible for fertility and in a ratio of 4-10 for the number of live births. In our experimental conditions, prolificacy would thus be more highly influenced by the buck than fertility. PMID:26795101

  14. Perfluorochemicals and Human Semen Quality: The LIFE Study

    OpenAIRE

    Louis, Germaine M. Buck; Chen, Zhen; Schisterman, Enrique F.; Kim, Sungduk; Sweeney, Anne M; Sundaram, Rajeshwari; Lynch, Courtney D.; Gore-Langton, Robert E.; Barr, Dana Boyd

    2014-01-01

    Background: The relation between persistent environmental chemicals and semen quality is evolving, although limited data exist for men recruited from general populations. Objectives: We examined the relation between perfluorinated chemicals (PFCs) and semen quality among 501 male partners of couples planning pregnancy. Methods: Using population-based sampling strategies, we recruited 501 couples discontinuing contraception from two U.S. geographic regions from 2005 through 2009. Baseline inte...

  15. New Approaches to Boar Semen Evaluation, Processing and Improvement.

    Science.gov (United States)

    Sutovsky, P

    2015-07-01

    The improvement of boar reproductive performance may be the next frontier in reproductive management of swine herd in Unites States, facilitated by better understanding of boar sperm function and by the introduction of new advanced instrumentation in the andrology field. Objective single ejaculate evaluation and individual boar fertility prediction may be possible by introducing automated flow cytometric semen analysis with vital stains (e.g. acrosomal integrity and mito-potential), DNA fragmentation analysis and biomarkers (ubiquitin, PAWP, ALOX15, aggresome) associated with normal or defective sperm phenotypes. Measurement of sperm-produced reactive oxygen species (ROS) is a helpful indicator of normal semen sample. Semen ROS levels could be managed by the addition of ROS-scavenging antioxidants. Alternative energy regeneration substrates and sperm stimulants such as inorganic pyrophosphate and caffeine could increase sperm lifespan in extended semen and within the female reproductive system. Such technology could be combined with timed sperm release in the female reproductive system after artificial insemination. Sperm phenotype analysis by the image-based flow cytometry will go hand in hand with the advancement of swine genomics, linking aberrant sperm phenotype to the fertility influencing gene polymorphisms. Finally, poor-quality ejaculates could be rescued and acceptable ejaculates improved by semen purification methods such as the nanoparticle-based semen purification and magnetic-activated sperm sorting. Altogether, these scientific and technological advances could benefit swine industry, provided that the challenges of new technology adoption, dissemination and cost reduction are met. PMID:26174914

  16. Hormonal induction and semen characteristics of tambaqui Colossoma macropomum.

    Science.gov (United States)

    Maria, Alexandre Nizio; Azevedo, Hymerson Costa; Santos, Jadson Pinheiro; Carneiro, Paulo César Falanghe

    2012-02-01

    In the hatchery-bred tambaqui Colossoma macropomum, spontaneous semen release does not occur, and hand-stripping produces reduced semen volume. The goal of this work is to evaluate the effects of hormonal induction with carp pituitary extract (CPE) on both qualitative (visual aspect, pH, motility, viability and morphological abnormalities) and quantitative (volume, concentration and number of spermatozoa per ejaculate) traits of tambaqui semen. Eleven males were treated with CPE (induced), and 11 were left untreated as a control (non-induced). All analysed parameters except motility and percentage of viable spermatozoa presented significant differences (p < 0.05) between the induced and non-induced treatments. CPE induction resulted in a 25-fold increase in semen volume and a 10-fold increase in the number of spermatozoa collected. However, both sperm concentration and the frequency of sperm with morphological abnormalities (commonly detached heads or bent tails) were significantly lower in CPE-induced fish. The hormonal induction of tambaqui males with CPE is efficient and positively influences some qualitative and quantitative properties of semen. Additionally, semen collection via gentle abdominal massage occurs more readily in CPE-induced fish. PMID:21208496

  17. Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC

    OpenAIRE

    Muhammad Rizal; Herdis .; Maman Surachman; W. Marlene Mesang-Nalley

    2008-01-01

    In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tube...

  18. Identifying factors affecting age at first semen freezing and age at first semen use in Sahiwal bulls

    OpenAIRE

    B. C. Naha; A. K. Chakravarty; M.A. Mir; V. Jamuna; A. P. Singh; Maher, D.

    2015-01-01

    Aim: The objective of the study was to evaluate the effects of non-genetic factors on reproduction traits viz. age at first semen freezing and age at first semen use of breeding bulls in Sahiwal bulls by fitting least-squares analysis. Materials and Methods: The information on reproduction traits of 43 Sahiwal breeding bulls belonging to 8 sets of Sahiwal breeding program at Indian Council of Agricultural Research-National Dairy Research Institute (ICAR-NDRI), Karnal (Haryana), India durin...

  19. Identifying factors affecting age at first semen freezing and age at first semen use in Sahiwal bulls

    Directory of Open Access Journals (Sweden)

    B. C. Naha

    2015-07-01

    Full Text Available Aim: The objective of the study was to evaluate the effects of non-genetic factors on reproduction traits viz. age at first semen freezing and age at first semen use of breeding bulls in Sahiwal bulls by fitting least-squares analysis. Materials and Methods: The information on reproduction traits of 43 Sahiwal breeding bulls belonging to 8 sets of Sahiwal breeding program at Indian Council of Agricultural Research-National Dairy Research Institute (ICAR-NDRI, Karnal (Haryana, India during 27 years (1987-2013 were analyzed using fixed linear model. The information was collected from AI records, reproduction sheets, and bull AI register maintained at different sections of Institute viz. record room of Dairy Cattle Breeding Division (DCB, Cattle Yard, Artificial Breeding Research Centre, ICAR-NDRI, Karnal. Results: The average age at first semen freezing and age at first semen use of Sahiwal breeding bulls was estimated as 3.170.01 years and 5.350.01 years, with the coefficient of variation 18.93% and 20%, respectively. The overall least squares mean for age at first semen freezing and age at first semen use was estimated as 3.140.09 years and 5.250.02 years, respectively, in Sahiwal breeding bulls. Period of freezing/use had significant effects on reproductive traits (p<0.01. Season had no significant effect on any of the traits considered in this study. Conclusion: It can be concluded that management inputs such as nutrition, breeding, and optimum environment should be taken care of to optimize age at first semen freezing and age at first semen use for better utilization of superior germplasm.

  20. Human semen assays for workplace monitoring. [Monitoring of hazardous materials by determining effects on semen of personnel

    Energy Technology Data Exchange (ETDEWEB)

    Wyrobek, A.J.; Gledhill, B.L.

    1978-11-07

    Decades of human semen studies have yielded compelling evidence that sperm can be used to access reproductive potential and diagnose pathology. With these studies as background, the small number of detailed semen studies of men exposed to physical and chemical agents point with optimism to the application of human semen assays as efficient, effective means to monitor for reproductive hazards in the workplace. Sperm are the most accessible of human gonadal tissue and provide a means of monitoring exposure induced changes in the human testes, changes which may result in infertility and increased frequencies of genetically abnormal gametes. The focus on semen has precipitated the development of new sperm bioassays which use older conventional andrological methods (i.e., sperm counts, motility, and morphology) as well as recently developed high speed flow and scanning methods for automated cytological analyses. The status of these sperm assays for workplace surveillance is reviewed, procedures are suggested with examples of use, and their effectiveness is evaluated. The available mouse models of induced semen changes are briefly described and the importance of these models for evaluating the genetic implications of findings in human semen is discussed.

  1. SEMEN QUALITY ASSESSMENT OF NEW ZEALAND WHITE RABBIT BUCKS

    Directory of Open Access Journals (Sweden)

    Martyna B?aszczyk

    2013-02-01

    Full Text Available Rabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detectedRabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detected.

  2. SEMEN QUALITY ASSESSMENT OF NEW ZEALAND WHITE RABBIT BUCKS

    Directory of Open Access Journals (Sweden)

    Martyna Błaszczyk

    2013-02-01

    Full Text Available Rabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37°C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detectedRabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37°C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detected.

  3. Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen.

    Science.gov (United States)

    Imrat, P; Suthanmapinanth, P; Saikhun, K; Mahasawangkul, S; Sostaric, E; Sombutputorn, P; Jansittiwate, S; Thongtip, N; Pinyopummin, A; Colenbrander, B; Holt, W V; Stout, T A E

    2013-02-01

    Semen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to compare the influence of extender (TEST versus INRA96®) and penetrating cryoprotectants (3% glycerol, 5% glycerol and 4% methylformamide) on post-thaw semen quality. We demonstrate that not only the freezing process, but also the quality of the semen before freezing, significantly influences the freezability of Asian elephant semen. Pre-freeze motility, viability, semen volume, semen pH, sperm concentration and the incidence of sperm mid-piece and tail abnormalities all significantly (pcryoprotectant did not significantly affect any of the above semen quality parameters post-thaw, the skim-milk based extender (INRA96®) preserved DNA integrity better (p<0.05) than the egg yolk extender (TEST). Considerable between-ejaculate variation in all post-thaw semen quality parameters was also noted. It is concluded that strict criteria for semen quality is essential for the selection of Asian elephant bull ejaculates suitable for cryopreservation; stricter initial selection should improve the mean post-thaw quality. PMID:23168056

  4. Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams

    Directory of Open Access Journals (Sweden)

    Rahmat Hidayat

    2016-01-01

    Full Text Available Contribution of Garut breed sheep as protein resources is not optimal yet. Acceleration of population growth such as increasing of female offspring is neccesary. This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K – (Cl+S/100 g of dry matter] and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows: RN0= basal ration (PKAR +14 without fish oil, RNI= basal ration (PKAR +14 with 3% fish oil, RB0= base ration (PKAR +40 without fish oil, RBI= base ration (PKAR +40 with 3% fish oil, RA0= acid ration (PKAR -40 without fish oil, and RAI= acid ration (PKAR -40 with 3% fish oil. The rations contained 150 ppm of zinc and were offered to 18 of Garut rams. The result indicated that blood pH, pCO2, and pO2 were not affected by PKAR and fish oil supplementation, but cHCO3 and cBase were affected (P<0.05. PKAR and fish oil supplementation affected (P<0.01 plasma Mg and S, but did not affect K, Na, Zn and Cl. Semen pH after day 28 of experimental period were highly significant different and that followed PKAR pattern. In conclution, PKAR affected cBase, cHCO3, concentration of Mg and S plasma and semen pH of Garut rams. The result suggested that PKAR could be applied to manipulate physiological condition and semen pH.

  5. Bulls grazing Kentucky 31 tall fescue exhibit impaired growth, semen quality, and decreased semen freezing potential.

    Science.gov (United States)

    Pratt, S L; Stowe, H M; Whitlock, B K; Strickland, L; Miller, M; Calcatera, S M; Dimmick, M D; Aiken, G E; Schrick, F N; Long, N M; Duckett, S K; Andrae, J G

    2015-02-01

    Serum prolactin (PRL) and testosterone concentrations, body weight, body composition, semen quality, and semen freezing potential for bulls grazing the toxic tall fescue (Lolium arundinaceum [Schreb.] Darbysh. = Schedonorous arundinaceum [Schreb.] Dumort.) cultivar Kentucky 31 (E+) compared with a novel endophyte cultivar lacking ergot alkaloids (E-) were evaluated. Angus bulls were allotted to treatment (Day 0) and grazed E+ or E- for 155 days. Treatment-by-day interaction was significant (P  0.05). Further, bulls on the E+ treatment exhibited decreased total gain, average daily gain, and body weight by Day 140 (P < 0.05) compared with the E- bulls. Rump muscle depth was lower because the treatment in bulls grazing E+ compared with E- (P < 0.05) and intramuscular fat in the E- bulls compared with the E+ group was higher by Day 155 (P < 0.05). Analysis of ejaculates showed significant treatment × day effects for sperm concentration with lower values observed for bulls on the E+ treatment (P < 0.05). The percent normal morphology was reduced in ejaculates from E+ bulls compared with E- bulls (P < 0.05), and the difference was due to an increase in abnormal sperm present in the E+ ejaculates from Day 84 to 140 (P < 0.05). In addition, spermatozoa motility and progressive motility were decreased on thawing in semen samples from E+ bulls compared with E- bulls (P < 0.05). PMID:25459421

  6. Thermal manipulation during embryogenesis improves certain semen parameters in layer breeder chicken during hot climatic conditions.

    Science.gov (United States)

    Shanmugam, M; Vinoth, A; Rajaravindra, K S; Rajkumar, U

    2015-10-01

    Thermal manipulation during incubation has been shown to improve post hatch performance in poultry. The aim of the present experiment was to evaluate thermal manipulation on semen quality of roosters during hot climatic conditions. Eggs obtained after artificial insemination from Dahlem Red layer breeders were randomly divided into two groups control (C) and heat exposed (HE). C group eggs were incubated at 37.5°C throughout the incubation period while the HE group eggs were exposed to higher temperature 40.5°C from 15th to 17th day of incubation for 3h each day. The relative humidity was maintained at 65% in both the groups throughout incubation. The chicks hatched were reared separately under standard husbandry conditions. During high ambient temperature semen from roosters (45 weeks of age) was collected and evaluated for different gross parameters, sperm chromatin integrity and sperm HSP27 and HSP70 gene expression by real-time PCR. The seminal plasma was evaluated for lipid peroxidation, ferric ion reducing antioxidant power (FRAP), triiodothyronine (T3) and matrix metalloproteinase-2 (MMP-2) activity. The shed average Temperature Humidity Index (THI) during the experiment period was 78.55. The percent live sperm and FRAP level were significantly (P<0.05) higher and sperm gene expressions were significantly (P<0.05) lower in the HE group. No differences in other parameters were observed between the groups. Thus from the results it could be concluded that thermal manipulation during incubation improves certain semen parameters of roosters at high ambient temperature. PMID:26386679

  7. Quality of semen after repeated ejaculation treatment in spinal cord injury men.

    Science.gov (United States)

    Siösteen, A; Forssman, L; Steen, Y; Sullivan, L; Wickström, I

    1990-02-01

    The study was designed to document the effects of regular drainage after penile vibrator stimulation on the quality of semen in SCI men. Twenty three tetraplegics and 9 paraplegics, 18 to 40 years of age and with neurological levels ranging from C4 to L1, were examined between 1 and 23 years after injury (median 2 years). None had ejaculated after the injury. Penile vibrator stimulation was tried in patients with cervical or thoracic lesions who showed reflex hip flexion on scratching the soles of the feet, 29 out of 32, and rectal electrostimulation in the remaining 3 low lesions with no reflex function in lumbar and sacral segments. The stimulation yielded semen in 29 (91%) of the men; 22 had antegrade and 7 retrograde ejaculation. Sixteen of the 22 patients with antegrade ejaculation entered a home programme of vibrator stimulation prescribed once weekly. Four to 6 months of stimulation resulted in a rise of semen volume and of fructose and acid phosphatase levels in the seminal plasma, suggesting improved function of the seminal vesicles and the prostate. The percentage of motile sperms was low both before and after the treatment period. Despite this, the total count of motile sperms per ejaculate was already high in the patient's first ejaculates compared to the laboratory normal standard, and further clearly increased after the stimulation period. A standardised functional test measuring sperm penetration capacity showed strong evidence of long term stimulation effect. It is suggested that the test is used in the assessment of fertility potential in SCI men. PMID:2235028

  8. Lifestyle and semen quality: role of modifiable risk factors.

    Science.gov (United States)

    Jurewicz, Joanna; Radwan, Michał; Sobala, Wojciech; Ligocka, Danuta; Radwan, Paweł; Bochenek, Michał; Hanke, Wojciech

    2014-02-01

    The relationship between exposure to lifestyle factors and adverse effects on human reproductive health is debated in the scientific literature and these controversies have increased public and regulatory attention. The aim of the study was to examine the association between modifiable lifestyle factors and main semen parameters, sperm morphology, and sperm chromatin structure. The study population consisted of 344 men who were attending an infertility clinic for diagnostic purposes with normal semen concentration of 20-300 M/ml or with slight oligozoospermia (semen total concentration of 15-20 M/ml) [WHO 1999]. Participants were interviewed and provided semen samples. The interview included questions about demographics, socio-economic status, medical history, lifestyle factors (consumption of alcohol, tobacco, coffee intake, cell phone and sauna usage), and physical activity. The results of the study suggest that lifestyle factors may affect semen quality. A negative association was found between increased body mass index (BMI) and semen volume (p = 0.03). Leisure time activity was positively associated with sperm concentration (p = 0.04) and coffee drinking with the percentage of motile sperm cells, and the percentage of sperm head and neck abnormalities (p = 0.01, p = 0.05, and p = 0.03, respectively). Drinking red wine 1-3 times per week was negatively related to sperm neck abnormalities (p = 0.01). Additionally, using a cell phone more than 10 years decreased the percentage of motile sperm cells (p = 0.02). Men who wore boxer shorts had a lower percentage of sperm neck abnormalities (p = 0.002) and percentage of sperm with DNA damage (p = 0.02). These findings may have important implications for semen quality and lifestyle. PMID:24074254

  9. Effect of exposing rams to a female stimulus before semen collection on ram libido and semen quality.

    Science.gov (United States)

    Fahey, A G; Duffy, P; Fair, S

    2012-10-01

    Rams with strong libido and desirable semen characteristics can provide more insemination doses per ejaculate and produce more progeny, improving population genetic linkage to improve the accuracy of EBV. The objective of this study was to determine if teasing rams, either by sight and smell alone (Exp. 1), or physical contact (Exp. 2), could improve libido and semen quality of rams. In Exp. 1, there were 3 treatments in which rams were exposed to the sight and smell of the ewe for 1 h: control treatment (n = 5) in which rams were exposed to a ewe not in estrus; non-novel treatment (n = 6) in which rams were exposed to a ewe in estrus and the same ewe was used for semen collection; and novel treatment (n = 6) in which rams were exposed to a ewe in estrus and a different ewe in estrus was used for semen collection. In Exp. 2, rams were individually given full access to a ewe, which had a cotton apron fitted to cover her vulva, for 15 min. The 3 treatments in Exp. 2 were: control treatment (n = 5) in which rams were placed in a pen with a ewe not in estrus; a non-novel treatment (n = 5) in which rams were placed in a pen with a ewe in estrus and the same ewe was used for semen collection; novel treatment (n = 6) in which rams were placed in a pen with a ewe in estrus and a different ewe in estrus was used for semen collection. Experiment 1 was repeated for 5 consecutive days and Exp. 2 was repeated for 4 consecutive days. Data on reaction time, number of mounts, semen volume, semen concentration, sperm wave motion, and progressive linear motion (Exp. 1 only) were collected and analyzed as a randomized complete block design, where rams were initially blocked for breed and age. In Exp. 1, there was an effect of day (P smell of a ewe in estrus. Alternatively, when rams were stimulated with physical contact of a ewe in estrus, an acute increase in semen volume was evident on d 1. These effects were not evident on subsequent days and thus the overall benefits on ram libido and semen quality of exposing rams to ewes in estrus are minimal. PMID:22665631

  10. Fertility of Cow in Using Locally Produced Chilled and Imported Frozen Semen

    Directory of Open Access Journals (Sweden)

    A. K. Das

    2002-01-01

    Full Text Available The experiment was carried out at Central Cattle Breeding Station and Dairy farm, Savar, Dhaka, and 3 sub- station and 9 points of Chandpur District in Bangladesh to evaluate the quality and fertilizing capacity of locally produced chilled and imported frozen semen. Motility, sperm concentration and mass activity of semen from different experimental bulls were almost similar. Quality of imported frozen semen was better than that of locally produced chilled semen in respect of motility, motile sperm/ Insemination dose and spermatozoa with normal head. Motility and pH value of semen decreased significantly for transportation and prolongation of preservation duration. Average conception rate of imported frozen semen (57.33 was found to be higher than locally produced chilled semen (45.33. But it was similar between imported frozen (57.33 and average of 1st & 2nd day preserved semen (57%.

  11. Semen quality in relation to antioxidant intake in a healthy male population

    DEFF Research Database (Denmark)

    Zareba, Piotr; Colaci, Daniela S; Afeiche, Myriam; Gaskins, Audrey J; Jørgensen, Niels; Mendiola, Jaime; Swan, Shanna H; Chavarro, Jorge E

    2013-01-01

    To assess the relationship between dietary antioxidant intake and semen quality in young healthy males.......To assess the relationship between dietary antioxidant intake and semen quality in young healthy males....

  12. Distinct Cytokine Patterns in Semen Influence Local HIV Shedding and HIV Target Cell Activation

    OpenAIRE

    Olivier, Abraham J.; Masson, Lindi; Ronacher, Katharina; Walzl, Gerhard; Coetzee, David; Lewis, David A.; Williamson, Anna-Lise; PASSMORE, Jo-Ann S.; BURGERS, Wendy A.

    2013-01-01

    Background.?Semen is the main vector for human immunodeficiency virus (HIV) transmission from men to women. We investigated the influence of cytokines in semen on local HIV burden and activated T cells.

  13. Maternal folic acid supplement intake and semen quality in Danish sons: a follow-up study

    DEFF Research Database (Denmark)

    Jacobsen, Kristoffer; Ramlau-Hansen, Cecilia Hst; Thulstrup, Ane Marie; Olsen, Jrn; Bonde, Jens Peter

    2011-01-01

    To examine whether maternal folic acid supplement intake during pregnancy is related to better semen quality in male offspring.......To examine whether maternal folic acid supplement intake during pregnancy is related to better semen quality in male offspring....

  14. Detection of Neospora caninum DNA in semen of experimental infected rams with no evidence of horizontal transmission in ewes.

    Science.gov (United States)

    Syed-Hussain, S S; Howe, L; Pomroy, W E; West, D M; Smith, S L; Williamson, N B

    2013-11-01

    Recent reports from New Zealand indicate Neospora caninum has a possible role in causing abortions in sheep. Transmission of N. caninum via semen has been documented in cattle. This study aimed to investigate if horizontal transmission through semen was also possible in sheep. Initially, 6-month old crossbred ram lambs (n=32), seronegative to N. caninum, were divided into 4 equal groups. Group 1 remained uninoculated whilst the remainder were inoculated with N. caninum tachyzoites intravenously as follows: Group 2 - 50 tachyzoites; Group 3 - 10(3) tachyzoites; Group 4 - 10(7) tachyzoites. Semen samples were collected weekly for 8 weeks for the detection of N. caninum DNA and quantified using quantitative PCR (qPCR). Plasma collected 1 month post-inoculation was subjected to ELISA (IDEXX Chekit) and Western blot. At 2 weeks post-infection, three rams from Group 1 (uninoculated) and three rams from Group 4 (10(7)tachyzoites/ml) were mated with two groups of 16 ewes over two oestrus cycles. Ewe sera collected 1 and 2 months post-mating were tested for seroconversion by ELISA and Western blot. All experimentally infected rams seroconverted by 1 month with ELISA S/P% values ranging from 11% to 36.5% in Group 2, 12-39.5% in Group 3 and 40-81% in Group 4. However, none of the ewes mated with the experimentally infected rams seroconverted. For the Western blot, responses towards immunodominant antigens (IDAs) were observed in ram sera directed against proteins at 10, 17, 21, 25-29, 30, 31, 33 and 37 kDa. Rams in Group 2, 3 and 4 were noted to have at least 3 IDAs present. None of the ewes showed any of the 8 prominent IDAs except for the one at 21 kDa which was seen in 30 out of 32 ewes in both groups. N. caninum DNA was detected intermittently in the ram's semen up to 5 weeks post-inoculation with the concentrations ranging from that equivalent to 1-889 tachyzoites per ml of semen. Low concentrations of N. caninum DNA were also detected in the brain tissue of two rams (Groups 1 and 4). These results suggest that although N. caninum DNA can be found in the semen of experimentally infected rams, the transmission of N. caninum via natural mating is an unlikely event. PMID:23819894

  15. Successful artificial insemination in the Asian elephant (Elephas maximus) using chilled and frozen-thawed semen

    OpenAIRE

    Wongkalasin Warut; Boonprasert Khajornpat; Rungsri Ronnachit; Jansittiwate Saran; Angkawanish Taweepoke; Pinyopummin Anuchai; Kornkaewrat Kornchai; Pongsopavijitr Pornsawan; Thitaram Chatchote; Mahasawangkul Sittidet; Thongtip Nikorn; Homkong Pongpon; Dejchaisri Suthathip; Wajjwalku Worawit; Saikhun Kulnasan

    2009-01-01

    Abstract Background Artificial insemination (AI) using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artificial insemination (AI). Methods Semen samples were collected by from 8 bulls (age range, 1...

  16. Association between environmental exposure to cadmium and human semen quality.

    Science.gov (United States)

    Li, Yuyan; Wu, Junqing; Zhou, Weijin; Gao, Ersheng

    2016-04-01

    Cadmium (Cd) is a heavy metal with toxicant to reproductive functions. The aim of this study was to explore the effects of environmental exposure to Cd on human semen quality. A total of 587 men from the general population, aged from 20 to 59years old, and without occupational exposure to Cd were recruited from three provinces in China to participate in the study. The median of serum Cd was 1.9?g/L (P25-P75:1.1-2.9). In case Cd was less than or equal to 6.3?g/L (P95) and the semen parameters were logarithmically transformed, the inverse associations between Cd and semen volume (-0.030.007), progressive motility (-0.010.004), and sperm morphology (-0.040.004) were found across the whole group, after adjusting for age group, occupation, season of semen sample collection, abstinence intervals, smoking, alcohol drinking, and body mass index. Our findings indicate that higher Cd may reduce the semen volume, progressive motility, and morphology among men without occupational exposure to Cd. PMID:26249156

  17. Rooster Semen Cryopreservation: Effect of Pedigree Line and Male Age on Post-Thaw Sperm Function

    Science.gov (United States)

    The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in preservation of commercial genetic stocks. Our objective was to evaluate the cryosurvival of semen from 8 pedigreed layer lines at the onset and end of production. Semen from 160 roosters (20/line) was...

  18. Semen cryopreservation in Bactrian camel (Camelus bactrianus) using SHOTOR diluent: effects of cooling rates and glycerol concentrations.

    Science.gov (United States)

    Niasari-Naslaji, A; Mosaferi, S; Bahmani, N; Gerami, A; Gharahdaghi, A A; Abarghani, A; Ghanbari, A

    2007-09-01

    Experiments were conducted with a final goal of providing a suitable protocol for cryopreservation of Bactrian camel semen. In Experiment I, the effect of average cooling rate (slow cooling: 0.14 versus fast cooling: 0.55 degrees C/min) on the viability of chilled semen was evaluated. In Experiment II, the effect of different concentrations of glycerol (4, 6 and 8%) on the post-thaw viability of frozen sperm was investigated. In Experiment III, the efficiency of SHOTOR diluent was compared with IMV buffers for the cryopreservation of camel semen. Viability parameters including progressive forward motility (PFM), plasma membrane integrity and percentage of live spermatozoa were assessed. Progressive forward motility of sperm cooled at the faster rate was superior after incubating for 24h at 4 degrees C compared to that cooled at the slower rate (Pcamel sperm was better using a final glycerol concentration of 6% compared to 4 and 8% (Psemen cryopreservation in Bactrian camel is feasible when it is extended in SHOTOR diluent, cooled within 1h (average cooling rate: 0.55 degrees C/min) to 4 degrees C, and then exposed to glycerol, at the final concentration of 6%. PMID:17588650

  19. Royal jelly supplementation in semen extender enhances post-thaw quality and fertility of Nili-Ravi buffalo bull sperm.

    Science.gov (United States)

    Shahzad, Qaisar; Mehmood, Muhammad Usman; Khan, Hamayun; Husna, Asma Ul; Qadeer, Saima; Azam, Asima; Naseer, Zahid; Ahmad, Ejaz; Safdar, Muhammad; Ahmad, Mushtaq

    2016-04-01

    Two experiments were conducted to evaluate the effect of royal jelly (RJ) on post-thaw sperm quality, in vitro and in vivo fertility rate of cryopreserved buffalo bull sperm. The semen was collected from three mature regular donor buffalo bulls, ejaculates were pooled and semen evaluated initially. In Experiment 1, the ejaculates were extended in tris-citric acid diluter supplemented with different RJ concentrations (0, 0.05, 0.1, 0.2, 0.3 or 0.4%). The diluted semen was cooled to 4°C, packaged into 0.5mL straws and frozen using standard procedure. The straws were thawed and assessed for sperm progressive motility, viability, plasma membrane, acrosome, and chromatin integrity. The results indicated that sperm progressive motility was significantly greater (Pplasma membrane and acrosome integrity were significantly improved (P0.05) between 0.1% RJ supplemented and control groups. It is concluded that supplementation of RJ in freezing extender can improve the cryosurvival rate and in vitro fertilizing capacity of buffalo bull sperm. PMID:26896924

  20. Metil-formamida na criopreservao de smen ovino / Methyl-formamide in ram semen cryopreservation

    Scientific Electronic Library Online (English)

    Carlos Pereira das, Graas; Alexandre In Piao Gomes, Lim; Andrei Antonioni Guedes, Fidelis; Jlio Roquete, Cardoso; Hlio, Blume; Rafael Gianella, Mondadori.

    2013-12-01

    Full Text Available Objetivou-se avaliar a eficcia da metil-formamida na criopreservao do smen ovino. O pool de smen utilizado no experimento foi obtido a partir da coleta, com vagina artificial, do smen de quatro carneiros mestios Santa Ins, com idade aproximada de quatro anos. As coletas foram realizadas uma [...] vez por semana, por seis semanas consecutivas, correspondendo, cada semana, a uma repetio do experimento. As fraes do pool foram diludas em cinco diferentes meios de congelao: (1) tris-gema com 5,3% de glicerol (TG5,3G); (2) tris-gema com 3% de metil-formamida (TG3MF); (3) tris-gema com 5% de metilformamida (TG5MF); (4) tris-gema com 7% de metil-formamida (TG7MF); (5) tris-gema com 9% de metil-formamida (TG9MF). Foram avaliadas a motilidade progressiva e o vigor das clulas espermticas e realizado o teste de termorresistncia ps-descongelao. O tratamento que obteve maior motilidade foi o TG5,3G (50%), seguido do TG3MF (38%) e os tratamentos que apresentaram menor motilidade progressiva foram TG5MF (29%), TG7MF (1,0%), TG9MF (6,0%). Os meios contendo metil-formamida apresentaram resultados inferiores ao meio controle para preservar a integridade morfolgica dos espermatozoides, sendo que nos meios TG7MF e TG9MF menos de 60% de espermatozides apresentaram-se morfologicamente normais. Os espermatozoides do meio TG5,3G apresentaram motilidade (15%) e vigor (2,8) similares aos do meio TG3MF (15% e 2,6, respectivamente) no teste de termorresistncia, mas o meio TG5,3G preservou melhor a integridade funcional da membrana plasmtica. O glicerol foi mais eficiente como crioprotetor do que a metil-formamida na criopreservao de smen ovino. Abstract in english The aim of this study was to evaluate the efficacy of methyl-formamide in ram semen cryopreservation. The semen pool used in this experiment was obtained by artificial vagina collection from four mixed breed Santa Ins rams, around four years of age. Semen collection was performed once a week, durin [...] g six weeks. Each week corresponded to one experiment replication. The semen pool was divided in five fractions in order to be diluted in one of the following freezing media: (1) tris-egg yolk with 5.3% of glycerol (TG5.3G); (2) tris-egg yolk with 3% of methyl-formamide (TG3MF); tris-egg yolk with 5% of methyl-formamide (TG5MF); tris-egg yolk with 7% of methyl-formamide (TG7MF); tris-egg yolk with 9% of methyl-formamide (TG9MF). Semen progressive motility, vigor and thermoresistance were evaluated. The treatments TG5.3G (50%) and TG3MF (38%) showed higher progressive motility after thawing, while TG5MF (29%), TG7MF (1%) and TG9MF (6%) showerd lower motility. Freezing media containing methyl-formamide were less effective in preserving spermatozoa membrane integrity and morphology than control media. In TG7MF and TG9MF extenders, less than 60% spermatozoa showed normal morphology. After thermoresistance test, semen cryopreserved in TG3MF showed vigor (2.6) and motility (15%) statistically similar to TG5.3G media (15% and 2.8, respectively); however, the extender TG5.3G was more effective in preserving plasma membrane functional integrity. In conclusion, in the experimental conditions used, glycerol showed more cryoprotectant potential than methyl-formamide.

  1. Effect of Ergosan on semen quality of male rainbow trout (Oncorhynchus mykiss) broodstock.

    Science.gov (United States)

    Sheikhzadeh, Najmeh; Reza, Asadpour; Allah, Jafari Jozani Razi; Hossein, Tayefi-Nasrabadi

    2010-12-01

    Present study was conducted to investigate the effect of Ergosan on seminal plasma compositions and spermatological parameters in rainbow trout. Male rainbow trout broodstocks (2300 200 g) were fed diets containing Ergosan at 2 different concentrations (6 mg kg(-1) and 20 mg kg(-1)) and control diet without Ergosan for 20 days and on day 22 fish semen were sampled. Results suggest that Ergosan in dietary intake, significantly increased the spermatocrit and sperm count in 20 mg kg(-1) group and Ca(2+) in both treatment groups compared to control group (P<0.05). The values of aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) had significant decrease in both treatment groups compared to the control group (P<0.05). Significant correlations were determined between sperm count versus K(+) value (r=-0.838, P<0.05) and glucose level (r=+0.835, P<0.05) in fish administrated with 20 mg kg(-1) of Ergosan. In group treated with 6 mg kg(-1), significant correlation between Na(+) and duration of sperm motility (r=+0.999, P<0.05) was shown. Meanwhile, glucose level versus percent of sperm motility (r=+0.866, P<0.05) showed significant correlation in this group. Sperm count versus total protein level (r=+0.817, P<0.05) showed significant correlation in control group. Results indicated that Ergosan had a potential efficacy on semen quality in rainbow trout broodstock. PMID:20810224

  2. Occupational exposure to pesticides and consequences on male semen and fertility: a review.

    Science.gov (United States)

    Mehrpour, Omid; Karrari, Parissa; Zamani, Nasim; Tsatsakis, Aristides M; Abdollahi, Mohammad

    2014-10-15

    Exposure to pesticides affects many body organs including reproductive system. Disorder of the reproductive system leads to infertility and therefore has been in the center of attention within the recent decades. Pesticides are one of the compounds that might reduce the semen quality in the exposed workers according to current knowledge. Although many underlying mechanisms have been proposed, the mechanisms of action are not clarified yet. The object of the present review was to criticize all the results of studies which evaluated the pesticide effects on male reproductive system. Results indicate that semen changes are multifactorial in the workers exposed to pesticides as there are numerous factors affecting sperm quality in occupational exposures. Majority of pesticides including organophosphoruses affect the male reproductive system by mechanisms such as reduction of sperm density and motility, inhibition of spermatogenesis, reduction of testis weights, reduction of sperm counts, motility, viability and density, and inducing sperm DNA damage, and increasing abnormal sperm morphology. Reduced weight of testes, epididymis, seminal vesicle, and ventral prostate, seminiferous tubule degeneration, change in plasma levels of testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH), decreased level and activity of the antioxidant enzymes in testes, and inhibited testicular steroidogenesis are other possible mechanisms. Moreover, DDT and its metabolites have estrogenic effects on males. Although effect of pesticides on sperm quality is undeniable, well-designed long-term studies are needed to elucidate all the possible affecting variables such as socioeconomic, cultural, nutritional, occupational, physical, and clinical characteristics alongside pesticides. PMID:24487096

  3. Effect of glycerol and dimethylformamide cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris diluents.

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    Ariantie OS

    2013-12-01

    Full Text Available A cryoprotectan is component that must be present in a cryopreservation medium to minimize the physical and chemical stresses resulting from the cooling, freezing and thawing of sperm cells. This study was carried out to determine the effect of glycerol (G and dimethylformamide (DMF as cryprotective agent in tris-egg yolk (TEY trehalose (T and tris-soya (TS raffinose (R diluents. Semen were collected from three sexually mature bucks using artificial vagina, evaluated and divided into four aliquot. Each of them was diluted with TEY suplemented with 50 mM trehalose and TS supplemented with 50 mM raffinose, added with glycerol or DMF 4% (v/v. Diluted semen was packed in minitube straw (100 x 106 sperm/0.25 mL and equilibrated for 4 hours at 5C, then freeze in N2 vapor for 10 minutes in styrofoam box and stored in liquid N2 container (-196 until futher evaluation. Progressive motility, viability and plasma membrane intact were evaluated after thawed at 37C for 30 seconds factorial experimental design (2 x 2 was used in this study. The sperm motility in TEYTG was significantly higher (65.075.38% compared to TEYTDMF (61.675.55%. In contrast sperm diluted with TSRDMF indicated better motility (42.228.13% than TSRG (39.075.38%. It was concluded that cryoprotectant had different effect on different diluents.

  4. Semen characteristics and refrigeration in free-ranging giant anteaters (Myrmecophaga tridactyla).

    Science.gov (United States)

    Luba, Camila do Nascimento; Boakari, Yatta Linhares; Costa Lopes, Alexandre Martins; da Silva Gomes, Marcelo; Miranda, Flvia Regina; Papa, Frederico Ozanan; Ferreira, Joo Carlos Pinheiro

    2015-12-01

    The giant anteater (Myrmecophaga tridactyla) is considered vulnerable to extinction. Scientific data on the reproductive parameters of this species are scarce. Semen from eight free-ranging giant anteaters was collected to establish its characteristics and the effects of cooling and storage at 5C after dilution with the BotuCrio extender without cryoprotectant. The ejaculate presented two distinct sequential fractions, including a whitish fraction, which was milky and rich in sperm cells, and a gel fraction, which was colorless, viscous, and azoospermic. The meanstandard error of the mean values of the seminal characteristics were as follows: volume of the first fraction, 0.750.1mL; motility, 752.9%; vigor, 3.20.3; sperm motility index, 68.84.3; concentration, 108.5 13.4נ10(6)/mL; plasma membrane integrity index, 714.0%; spermatic defects detected using modified Karras staining, 35.53.3%; and spermatic alterations identified by differential interference contrast microscopy, 48.36.8%. During refrigeration, the semen presented decreasing motility from 0 to 18hours, sperm motility index decreased from 0 to 24hours, and vigor did not change in the first 6hours and then decreased to 18hours. PMID:26376226

  5. Data for chicken semen proteome and label free quantitative analyses displaying sperm quality biomarkers

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    Valérie Labas

    2014-12-01

    Full Text Available Understanding of biology of the avian male gamete is essential to improve the conservation of genetic resources and performances in farming. In this study, the semen proteome of the main domestic avian species (Gallus gallus and evaluation of the molecular phenotype related to sperm quality were investigated using GeLC–MS/MS approach and label-free quantitative proteomic based on Spectral Counting (SC and extracted ion chromatograms (XIC methods. Here we describe in details the peptide/protein inventory of chicken ejaculated spermatozoa (SPZ and seminal plasma (SP. We also show differential analyses of chicken semen (SPZ and corresponding SP from 11 males demonstrating different levels of fertilizing capacity and sperm motility. The interpretation and description of these data can be found in a research article published by Labas and colleagues in the Journal of Proteomics in 2014 [1]. This is a new resource for exploring the molecular mechanisms involved in fertilizing capacity and to reveal new sets of fertility biomarkers.

  6. Data for chicken semen proteome and label free quantitative analyses displaying sperm quality biomarkers.

    Science.gov (United States)

    Labas, Valrie; Grasseau, Isabelle; Cahier, Karine; Gargaros, Audrey; Harichaux, Grgoire; Teixeira-Gomes, Ana-Paula; Alves, Sabine; Bourin, Marie; Grard, Nadine; Blesbois, Elisabeth

    2014-12-01

    Understanding of biology of the avian male gamete is essential to improve the conservation of genetic resources and performances in farming. In this study, the semen proteome of the main domestic avian species (Gallus gallus) and evaluation of the molecular phenotype related to sperm quality were investigated using GeLC-MS/MS approach and label-free quantitative proteomic based on Spectral Counting (SC) and extracted ion chromatograms (XIC) methods. Here we describe in details the peptide/protein inventory of chicken ejaculated spermatozoa (SPZ) and seminal plasma (SP). We also show differential analyses of chicken semen (SPZ and corresponding SP) from 11 males demonstrating different levels of fertilizing capacity and sperm motility. The interpretation and description of these data can be found in a research article published by Labas and colleagues in the Journal of Proteomics in 2014 [1]. This is a new resource for exploring the molecular mechanisms involved in fertilizing capacity and to reveal new sets of fertility biomarkers. PMID:26217683

  7. Evaluation of semen parameters in semen donors in a ten-year period in the city of So Paulo

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    Sidney Glina

    2010-12-01

    Full Text Available Objective: To evaluate sperm concentration, morphology and motility of Brazilian semen donors from 1992 to 2003, in the city of So Paulo. Methods: Retrospective study analyzing 182 donor semen samples from 1992 to 2003. The first and the second donated sample were analyzed for each donor. Donor average age was 30.8 years. Means with standard errors, medians with minimum and maximum values, and interquartile ranges were calculated for age, sperm concentration, semen volume, oval morphology and motility. The relation between each characteristic of the semen samples and the year of donation, as well as donor age and season of the year were studied by linear and multiple regression analysis. Results: Linear regression analysis showed that the sperm concentration (R2 = 19.1%, R2 = 20.2%, p < 0.0001 respectively and the oval morphology (R2 = 13%; R2 = 13.5%; p < 0.0001, respectively decreased significantly, even when the first or the second sperm collection is considered. The ejaculated volume showed slight increase during the period for both samples (R2 = 2.2%, p = 0.048; R-sq = 2.4%. p = 0.038, respectively. All characteristics did not depend on the donors age or season of the year when the samples were obtained. Conclusions: There was a decrease in spermatic concentration and percentage of oval sperm of semen donors samples from 1992 to 2003, in the city of So Paulo.

  8. Relationships among frozen-thawed semen fertility, physical parameters, certain routine sperm characteristics and testosterone in breeding Murrah buffalo (Bubalus bubalis bulls

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    A. K. Singh

    2014-09-01

    Full Text Available Aim: The present study was carried out to examine the relationships among frozen-thawed semen fertility, physical parameters, seminal quality, and testosterone concentration in Murrah buffalo bulls. Materials and Methods: A total of 30 breeding Murrah buffalo bulls (either progeny tested or under progeny testing program were randomly selected from two government bull farms in Punjab. None of the bulls selected for this study had any preceding physical abnormality. A field fertility trial was conducted to determine the first service conception rate (FSCR. The number of females inseminated per bull semen was 10. All the bulls were inspected for structural soundness, measurement of scrotal circumference, testicular biometry, and internal pelvic area (IPA. Frozen-thawed semen was evaluated for total motility, progressive motility, viability, concentration, abnormality, and hypo-osmotic swelling test (HOST. Testosterone was estimated in blood plasma, seminal plasma as well as frozen-thawed semen extracts for establishing relationship. Results: The FSCR was 48% in the bulls having a scrotal circumference of ≥44 cm, although, there was no significant correlation between FSCR and scrotal circumference. Similarly, no consistent relationship existed between sperm concentration and scrotal circumference. A positive correlation was observed between IPA and FSCR (r=0.294. Of the six post-thaw seminal components (total motility, progressive motility, viability, HOST (%, total abnormality and concentration only total motility had a high significant (p<0.01 correlation with FSCR (r=0.694. Varied correlations existed between other seminal parameters and fertility. Using a simple regression analysis, the post-thaw motility, IPA, prepuce length and testosterone (independent variables combined to explain approximately 62% of the variation in the FSCR (dependent variable. Conclusion: The present study indicated that despite low to high correlations between seminal characteristics, physical parameters, fertility, and testosterone; the observations support the importance of these components and their function in maintaining semen quality and subsequent fertility.

  9. Sperm penetration assay and its correlation with semen analysis parameters

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    Laxmi Kant Pandey

    2015-11-01

    Full Text Available Background: Aim of current study was to determine whether the Sperm Penetration Assay (SPA can be used as a test to discriminate the infertile male from fertile one. We have also correlated the SPA with semen analysis. Methods: Sperm characteristics namely Semen analysis and the sperm penetration assay were tested in 44 infertile and 10 fertile men. Sperm penetration assay was determined by using zona free hamster eggs. Results: With decreasing spermatozoa concentration in the semen there was significant decrease in percentage penetration of zona free Hamster eggs (p0.05. Conclusions: The Sperm penetration assay could discriminate the infertile group from fertile group significantly (p<0.001. The test appeared to be highly reproducible and probably identifies a truly infertile male. [Int J Res Med Sci 2015; 3(11.000: 3197-3201

  10. Effectiveness of a tris-based extender (SHOTOR diluent) for the preservation of Bactrian camel (Camelus bactrianus) semen.

    Science.gov (United States)

    Niasari-Naslaji, A; Mosaferi, S; Bahmani, N; Gharahdaghi, A A; Abarghani, A; Ghanbari, A; Gerami, A

    2006-08-01

    The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of Bactrian camel semen at 4 degrees C during 24 h. In experiments I and II, the effects of varying osmolalities (270, 300, 330, 360, and 390 mOsm/kg) and pHs (5.5, 6, 6.9, 7.5, 7.9, and 8.9) of tris-based extender on sperm viability were investigated. In experiment III, the efficiency of tris-based extender (SHOTOR diluent) in preserving Bactrian camel semen was compared with lactose (10%), sucrose (10%) and Green buffer. Viability parameters including progressive forward motility (PFM), plasma membrane integrity and the percentage of live spermatozoa were assessed. The data were analyzed using general linear model procedure. In the majority of assessments using tris-based extender, the viability of spermatozoa was superior at the osmolality of 330 mOsm/kg and pH of 6.9. PFM was significantly greater at the time of semen dilution in tris-based (65.5%) and Green buffer (60.5%) compared to that of lactose (31%) and sucrose (28%) extenders (P0.05). In conclusion, the utilization of a tris-based extender, having the osmolality of 330 mOsm/kg and pH of 6.9, favors the short-term preservation of the Bactrian camel spermatozoa under chilled condition. PMID:16647050

  11. Effects of Seasonal Changes and Shearing on Thermoregulation, Blood Constituents and Semen Characteristics of Desert Rams (Ovis aries

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    M. Abdelatif Abdalla

    2013-01-01

    Full Text Available This experiment was designed to study the effects of shearing in different seasons (winter vs. summer on thermoregulation, blood parameters and semen characteristics of desert rams. Eight intact healthy rams were randomly assigned into two groups (n = 4. The control group was kept unshorn (UN with intact pelage, the mean length of hair left was approximately 1.5 cm and the treated group was shorn (SH. Rectal temperature (Tr and Respiration Rate (RR measurements were carried out twice daily throughout the experimental period. Blood samples were collected once weekly for the evaluation of Packed Cell Volume (PCV, Total (TLC and Differential (DLC leukocyte count, Serum Total Protein (STP, Serum Albumin (SA, Serum Urea (SU and Plasma Glucose (PG concentration. Semen samples were collected once weekly for the determination of Ejaculate Volume (EV, Sperm Mass (SM and individual (SIM motility, Sperm Cell Concentration (SCC, live (LSP and abnormal (ABS sperm percent and semen pH. Scrotal Circumference (SC measurements were performed weekly. Shearing of desert rams significantly lowered the morning Tr in both seasons and the afternoon Tr during summer ,while RR was significantly lower in both seasons in the afternoon. The PCV was significantly lower in shorn rams during summer compared to winter and PG was significantly higher during winter compared to summer. In both seasons shearing significantly lowered SIM. It is concluded that shearing significantly affected thermoregulation, blood composition and semen characteristics during winter and summer. It is concluded that shearing in different season significantly affected thermoregulation, blood parameters and seminal traits of Desert Hamari rams.

  12. Does exposure to di(2-ethylhexyl) phthalate in pre-pubertal boars affect semen quality post-puberty?

    Science.gov (United States)

    Spjuth, Linda; Ljungvall, Karl; Saravia, Fernando; Lundeheim, Nils; Magnusson, Ulf; Hultn, Fredrik; Rodrguez-Martnez, Heriberto

    2006-10-01

    Di(2-ethylhexyl) phthalate (DEHP), a plastic softener used in polyvinyl chloride (PVC) products, has been ascribed to have toxic effects on animal reproduction. The present study aimed at determining potential late effects of pre-pubertal oral exposure to DEHP on semen quality in young pigs. Ten pairs of cross-bred male siblings were used. One brother in each pair became, at random, the test animal while the other acted as control. Test males were exposed to 300 mg/kg body weight (bw) of DEHP administered orally three times a week from 3 to 7 weeks of age. The control group was given placebo (water). Semen analyses started when the boars reached 6 months of age, with semen collected twice weekly, until animals were 9 months of age. Semen was evaluated for ejaculate volume, sperm concentration, total sperm count, sperm motility, sperm morphology (including presence of cells other than spermatozoa) and sperm plasma membrane integrity. Total sperm motility tended to be lower while local motility was higher in the DEHP-exposed group compared with controls (p = 0.07) when assessed by computer-assisted sperm analysis. The DEHP-exposed group had a significantly (p < 0.05) lower percentage of spermatozoa with tailless, defective heads (at 7-8 months of age) and double-folded tails (at 6-7, 7-8 and 6-9 months of age), compared with controls (albeit always under 5%). In summary, there were no obvious adverse effects of early oral exposure to 300 mg/kg bw of DEHP on sperm output and sperm quality in post-pubertal young boars. PMID:16637905

  13. Decreased levels of genuine large free hCG alpha in men presenting with abnormal semen analysis

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    Plas Eugen

    2011-08-01

    Full Text Available Abstract Background The pregnancy hormone human chorionic gonadotropin (hCG and its free subunits (hCG alpha, hCG beta are produced in the male reproductive tract and found in high concentrations in seminal fluid, in particular hCG alpha. This study aimed to elucidate changes in peptide hormone profiles in patients showing abnormal semen analyses and to determine the genuineness of the highly abundant hCG alpha. Methods Seminal plasma was obtained from 45 male patients undergoing semen analysis during infertility workups. Comprehensive peptide hormone profiles were established by a panel of immunofluorometric assays for hCG, hCG alpha, hCG beta and its metabolite hCG beta core fragment, placental lactogen, growth hormone and prolactin in seminal plasma of patients with abnormal semen analysis results (n = 29 versus normozoospermic men (n = 16. The molecular identity of large hyperglycosylated hCG alpha was analyzed by mass-spectrometry and selective deglycosylation. Results hCG alpha levels were found to be significantly lower in men with impaired semen quality (1346 +/- 191 vs. 2753 +/- 533 ng/ml, P = 0.022. Moreover, patients with reduced sperm count had reduced intact hCG levels compared with normozoospermic men (0.097 +/- 0.022 vs. 0.203 +/- 0.040 ng/ml, P = 0.028. Using mass-spectrometry, the biochemical identity of hCG alpha purified from seminal plasma was verified. Under non-reducing conditions in SDS-PAGE, hCG alpha isolated from seminal plasma migrated in a manner comparable with large free hCG alpha with an apparent molecular mass (Mr, app of 24 kDa, while hCG alpha dissociated from pregnancy-derived holo-hCG migrated at approximately 22 kDa. After deglycosylation with PNGase F under denaturing conditions, all hCG alpha variants showed an Mr, app of 15 kDa, indicating identical amino acid backbones. Conclusions The findings indicate a pathophysiological relevance of hCG, particularly its free alpha subunit, in spermatogenesis. The alternative glycosylation pattern on the free large hCG alpha in seminal plasma might reflect a modified function of this subunit in the male reproductive tract.

  14. The Relationship between Occupation and Semen Quality

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    Mohammad Hossein Vaziri

    2011-01-01

    Full Text Available Background: Infertility can be a major concern for couples trying to conceive, and occupationalhazards may constitute a main cause of infertility in men. Studies conducted throughout the worldindicate that physical and chemical hazards in the workplace can have a negative impact on malefertility. The main objective of this study was to determine the frequency of occupational categoriesof men who attended an infertility clinic, and to evaluate the differences in the semen qualityparameters among occupational categories.Materials and Methods: This cross-sectional study was conducted on 1164 males who werereferred to the Infertility Research Center in Tehran for treatment of infertility in order to evaluatethe effects of certain occupations on infertility. The participants were divided into several categoriesaccording to their occupations and evaluated by means of a questionnaire for duration of infertility,BMI, sperm count, percentage of normal sperm morphology and percentages of sperm with class Aand class B motilities. Descriptive statistics, analysis of variance, and correlations were conductedusing SPSS 16.0 for Windows.Results: There were no statistically significant differences in the mean sperm count or spermmorphology between occupational categories. Assessment of the differences in the frequency ofsperm motility classes between occupational categories revealed a significant difference only inthe frequency of sperm with class B motility. The lowest mean percentages of sperm with class Bmotility were seen in those involved in the transportation industry, a finding in agreement with anumber of other researches.Conclusion: Our findings revealed an association between occupation and sperm motility. Sinceour study population was relatively small and in many cases exposures to work hazards were brief,a larger study group must be evaluated in order to support the preliminary results of this study.

  15. The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies

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    Sahereh Joezy-Shekalgorabi

    2014-01-01

    Full Text Available A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC. Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC, continuous use of sexed semen only (SS, utilization of sexed semen for both the first and second services with conventional semen afterwards (S2, and utilization of sexed semen for the first service with conventional semen afterwards (S1. Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer herd.

  16. The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies

    Energy Technology Data Exchange (ETDEWEB)

    Joezy-Shekalgorabi, S.; Shadparvar, A. A.; Vries, A. de; Gay, K. D.

    2014-06-01

    A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC). Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC), continuous use of sexed semen only (SS), utilization of sexed semen for both the first and second services with conventional semen afterwards (S2), and utilization of sexed semen for the first service with conventional semen afterwards (S1). Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer herd. (Author)

  17. Semen quality in Schistosoma haematobium infected men in Madagascar

    DEFF Research Database (Denmark)

    Leutscher, Peter; Hst, Erik; Reimert, Claus M

    2009-01-01

    The seminal vesicles and the prostate are frequently affected by egg-induced inflammation in Schistosoma haematobium infected men. The objective of this study was to assess the semen quality in men with male genital schistosomiasis (MGS). The examination of the semen samples was performed in men...... aged 15 to 49 years living an S. haematobium endemic area in Madagascar prior to anti-schistosoma treatment with praziquantel and five months later. Men from the high endemic Sirama sugarcane plantation with positive egg excretion in the urine and circulating anodic antigen (CAA) present in serum (n=85...

  18. The Therapeutic Effects of a Medicinal Plant Mixture in Capsule Form on Catalase Levels in the Semen of Men with Oligospermia

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    Alizadeh Hasti

    2015-01-01

    Full Text Available Objective: In the present study, the therapeutic effects of mixed herbs (onion, ginger, basil, cinnamon, orange peel, yellow and red watermelon seeds, and carrot seed on catalase levels in the semen of men with oligospermia were evaluated. About 50% of recognized infertility factors are male-related factors, and are mainly the result of oligospermia, astenospermia, and teratozoospermia. Materials and Methods: The study participants included 40 males with oligospermia and infertility. The studied medicine were 700 mg capsules containing onion, ginger, basil, cinnamon, orange peel, yellow and red watermelon seeds, and carrot seed (100 mg of each. Catalase activity was measured by Aebi method. Results: A significant increase was observed in catalase level in semen as a result of using the medicinal plant mixture (P < 0.001. Conclusion: Free radicals play an important role in male infertility. Antioxidants can prevent the damaging effects they have on sperm. Oxidative stress reduction can increase the chances of natural fertility or assisted reproductive technology (ART. Medicinal plants have low costs, complications, and easy availability, and cause an increase in semen plasma antioxidants and subsequent improvement in semen parameters. Thus, they can be the source of new hopes for the treatment of infertility.

  19. Semen banking: consideration on viral contamination in the era of new emerging viral infection

    OpenAIRE

    Viroj Wiwanitkit

    2011-01-01

    To construct a semen bank, the collection of donated semen has to be done and an important concern is the safety of collected semen. The contamination is a big problem. Basically, the infectious pathogens can exist within donated semen, hence, a good donor screening is very important. Although viruses have an indirect role in sperm quality, but the evidence in banked semen is presently lack. This does not mean that there is no viral contamination but it might imply the inadequate concern on t...

  20. Effects of Vitamin E Addition to Chicken Semen on Sperm Quality During in Vitro Storage of Semen

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    Saleh Tabatabaei

    2011-06-01

    Full Text Available The purpose of this study was to evaluate the probable effects of the vitamin E addition in different levels to the extender of chicken semen on spermatozoa quality during storage of semen at 4°C for 0, 3, 6, 10 and 24 hours. Eight young Ross broiler breeder strain 308 roosters were used in this experiment. The collected semen from all roosters was mixed together and diluted with modified a Ringer’s solution. The diluted pooled semen was divided into 5 treatments (T. T1 was a control group without any vitamin E addition. For T2 to T5 groups 0.5 %, 1 %, 2 % and 3 % vitamin E (w/v, were added respectively. Treatments were evaluated for sperm motility, sperm viability and probable morphological defects after 0, 3, 6, 10 and 24 hours of incubation at 4°C. The evaluations of spermatozoa immediately after semen collection, were revealed no significant differences among values of treatment groups, whereas after incubating the treatments for different spans of time, the sperm progressive motility and viability rates for groups supplemented with vitamin E were significantly (P < 0.05 higher than that of the control group. In addition, morphological defect rates of chicken spermatozoa in the groups supplemented with different levels of vitamin E were significantly (P < 0.05 lower than that in control group. According to the results of this study we conclude that, the most excellent level of vitamin E for supplementation to the extended semen of chicken in order to improve the sperm motility and viability plus to reduce the morphological defect rates of the spermatozoa up to 24 hours storage time at 4°C is 2 % (w/v.

  1. Blood and Semen Selenium Concentrations and Semen Quality in Boars Fed Diets Supplemented with Organic or Inorganic Selenium

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    Theera Rukkwamsuk

    2012-01-01

    Full Text Available Effect of dietary supplementation of organic or inorganic selenium on blood and semen selenium concentrations and semen quality was determined in 10 boars. During the 4 weeks of pre-experimental period, all boars were fed a basal diet containing 0.15 mg kg-1 of inorganic selenium. Thereafter, all cows were randomly allocated into 2 groups of five boars which were fed a basal diet supplemented with either 0.3 mg kg-1 of inorganic selenium or 0.3 mg kg-1 of organic selenium for 84 days. Blood samples were collected from all boars to determine selenium concentrations at the end of pre-experimental period and at days 49 and 84 after supplementation. Semen samples were collected at the end of pre-experimental period and at days 35, 49, 63 and 84 to determine selenium concentrations and semen evaluation. For both inorganic and organic selenium groups, blood selenium concentrations at days 49 and 84 were higher than the concentration at day 0 and the concentrations did not differ between the two groups at all sampling periods. Semen selenium concentrations at days 35, 49, 63 and 84 were higher than the concentration at day 0 for both inorganic and organic selenium groups and the concentrations did not differ between the 2 groups at days 35, 49, 63 and 84. Sperm motility parameters including motility (%, progressive motility (%, Average Path velocity (VAP, ?m sec-1, Straight-line velocity (VSL, ?m sec-1 and Curvilinear velocity (VCL, ?m sec-1 did not differ between the 2 groups and among sampling periods. Results revealed that 0.3 mg kg-1 supplementation of either inorganic or organic selenium form in the basal diet containing 0.15 mg of selenium per kg could increase blood and semen selenium levels in the boars. With normally-fertile boars, both inorganic and organic form of selenium supplemented in the diet had similar effect on sperm motility characteristics in the boars.

  2. New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen.

    Science.gov (United States)

    Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

    2014-02-01

    Centrifugation of Apis mellifera L. drone semen is a necessary step in the homogenization of semen pools for the enlargement of the effective breeding population, as well as in the collection of semen by the so-called washing technique. It is also of interest for the removal of cryoprotectants after cryopreservation. The adoption of methods involving semen centrifugation has been hampered by their damaging effect to sperm. Here, we tested four new diluents as well as three additives (catalase, hen egg yolk, and a protease inhibitor), using sperm motility and dual fluorescent staining as indicators of semen quality. Three of the new diluents significantly reduced motility losses after centrifugation, as compared with the literature standard. Values of motility and propidium iodide negativity obtained with two of these diluents were not different from those measured with untreated semen. The least damaging diluent, a citrate-HEPES buffer containing trehalose, was then tested in an insemination experiment with centrifuged semen. Most queens receiving this semen produced normal brood, and the number of sperm reaching the storage organ of the queen was not significantly different from that in queens receiving untreated semen. These results could improve the acceptance of techniques involving the centrifugation of drone semen. The diluent used in the insemination experiment could also serve as semen extender for applications not involving centrifugation. PMID:24665683

  3. Use of Existing Diagnostic Reverse-Transcription Polymerase Chain Reaction Assays for Detection of Ebola Virus RNA in Semen.

    Science.gov (United States)

    Pettitt, James; Higgs, Elizabeth S; Adams, Rick D; Jahrling, Peter B; Hensley, Lisa E

    2016-04-15

    Sexual transmission of Ebola virus in Liberia has now been documented and associated with new clusters in regions previously declared Ebola free. Assays that have Emergency Use Authorization (EUA) and are routinely used to detect Ebola virus RNA in whole blood and plasma specimens at the Liberian Institute for Biomedical Research were tested for their suitability in detecting the presence of Ebola virus RNA in semen. Qiagen AVL extraction protocols, as well as the Ebola Zaire Target 1 and major groove binder quantitative reverse-transcription polymerase chain reaction assays, were demonstrably suitable for this purpose and should facilitate epidemiologic investigations, including those involving long-term survivors of Ebola. PMID:26374912

  4. Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen

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    O I Azawi

    2012-04-01

    Full Text Available The objectives of the present study were to determine the effects of six different antibiotics in controlling the growth of semen contaminating bacteria and if these antibiotics have any adverse effect on Awassi ram spermatozoa. Semen samples from six mature Awassi rams were used in this study. A total number of 120 ejaculates were collected from the rams using an artificial vagina once a week. Semen ejaculates were evaluated for volume, sperm concentration, mass motility, individual motility, percentage live sperm, sperm abnormalities, and viable bacterial count. Semen samples were diluted by sodium citrate-fructose-egg yolk. The diluted semen sample was divided into 7 parts. Six types of antibiotics were added to the semen diluent parts including; penicillin G 1000 IU ml-1 with streptomycin 1 mg ml-1, gentamicin sulphate 250 mg ml-1, tetracycline 0.5 mg ml-1, lincomycin 1 mg ml-1, cefoperazone sodium 1mg ml-1, cefdinir 1 mg ml-1 and the seventh part considered as a control group without antibiotic addition. The diluted semen samples were cooled and preserved at 5 Co for 5 days. Cooled diluted semen samples were examined for individual motility, percent of live sperm, sperm abnormalities, acrosomal defects and bacterial count every 24 h until 5 days. Comparing with the control, all the antibiotics examined were effective in controlling bacterial growth (P<0.05 from 24 h to 96 h of preservation at 5 Co. Cefdinir and cefoperazone sodium proved to be significantly (P<0.05 effective than other antibiotics in controlling bacterial growth at 96 h of preservation as the bacterial count were 23.3 ± 3.7 x 103 / ml and 25.4 ± 6.2 x 103 / ml, respectively. Lincomycin, gentamicin sulphate and tetracycline proved ineffective in controlling bacterial growth at 96 h of preservation as the bacterial count were 57.1 ± 20.1 x 103 / ml, 52.5 ± 29.4 x 103 / ml and 46.5 ± 8.8 x 103 / ml, respectively. The addition of tetracycline to diluted ram semen significantly reduced (P<0.05 sperm individual motility and percent live sperm and a significant increase (P<0.05 acrosomal defects was observed at 96 h of preservation in comparison to control and other antibiotics. Sperm viability was highly correlated with bacterial count in the control part of diluted semen (r = 0.794; P < 0.01. It could be concluded from the results of the present study that additions of cephalosporins (cefdinir or Cefoperazone sodium at the dose of 1 mg ml-1 were most effective amongst the antibiotics used in checking the bacterial growth and improving semen quality of Awassi ram. [Vet. World 2012; 5(2.000: 75-79

  5. Twin pregnancy possibly associated with high semen quality

    DEFF Research Database (Denmark)

    Asklund, Camilla; Jensen, Tina Kold; Jrgensen, Niels; Tabor, Ann; Sperling, Lene; Skakkebk, Niels Erik

    2007-01-01

    BACKGROUND: Recent studies found an association between a long waiting time to pregnancy (TTP) and reduced probability of twinning and a reduced dizygotic (DZ) twinning rate in subfertile men. However, it remains unsolved whether semen quality is associated with twin offspring. We therefore studied...

  6. [Comparison of phospholipid in crude and fried semen Dolichos Lablab].

    Science.gov (United States)

    Xu, Y; Wang, Y; Tao, C

    1991-09-01

    A study of the chemical changes of phospholipid in crude and fried semen Dolichos Lablab was carried out by molybdenum blue colorimetry and TLC scanning. The result shows that both the total phospholipid contents and the molar fraction of phosphatidylcholine in the fried samples are decreased as compared with the crude ones. PMID:1804199

  7. THE CURRENT STATE OF SEMEN STORAGE AND AI TECHNOLOGY

    Science.gov (United States)

    Turkeys are the only commercial livestock species completely dependent upon artificial insemination (AI) for fertile egg production. Given that every breeder hen must be inseminated weekly during egg production, AI is both time and labor-intensive. Methods for the timing, frequency, semen dosage a...

  8. Seasonal variation in protein profiles and HSP70 of Holstein crossbred bull semen

    International Nuclear Information System (INIS)

    Since HSP70 is the stress response protein, the impact of heat stress on semen quality may be displayed through the expression of protein profile and HSP70. This study investigated the seasonal effects on the protein profiles and HSP70 in spermatozoa and seminal plasma of 10 Holstein crossbred bulls from an AI centre located in Lopburi, Thailand. Bull semen was collected weekly for 8 consecutive weeks during rainy (average THI 79.34), cool (average THI 75.27), and summer (average THI 80.10) seasons. Protein was extracted from both spermatozoa and seminal plasma using Laemmli's sample buffer. The protein profiles of spermatozoa and seminal plasma were subjected to one-dimensional SDSPAGE with 12% (w/v) acrylamide gel and 4.0% (w/v) acrylamide stacking gel for 120 min. at 8 mA. To visualize the protein profiles, gels were fixed in acetic acid: ethanol: H2O (7: 40: 53), stained with 0.125% (w/v) Coomassie blue R-250 in acetic acid: ethanol: H2O (7: 40: 53) for 60 min., and distained with acetic acid: ethanol: H2O (11: 26: 63) until the background was clear. Western blotting, as described by Kamaruddin et al. was conducted to determine HSP70 using anti-HSP70 monoclonal antibody. Proteins in the polyacrylamide gel were electrophoretically transferred, for 90 min. at 156 mA, to a PVDF membrane. The membrane was rinsed in PBS and blocked overnight in a blocking solution (advanced ECL blocking; Amersham Life Science Inc., Oakville, ON, Canada). The membrane was then incubated for 1 h at room temperature with monoclonal anti-HSP70 (H5147 Sigma Chemical Supplies CO., LTD), incubated with anti-mouse IgG horse radish peroxidase conjugated for 1 h at room temperature, and then detection for immunoreactive bands using ECL detection reagents (Amersham Life Science Inc.) on scientific imaging film. It was found that the profiles of protein were not different among seasons in both sperm and seminal plasma. The profiles of spermatozoa protein range from 10 to 220 kDa while most of proteins found in seminal plasma were low molecular weight (14-30 kDa). The HSP70 was found in both sperm and seminal plasma. However, the amount of HSP70 in winter appears to be greater compare to those found in summer and rainy seasons

  9. Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender.

    Science.gov (United States)

    Eidan, Sajeda M

    2016-04-01

    This study was undertaken to investigate the influence of adding combinations of vitamin C to Tris extender with either catalase or reduced glutathione on post-cryopreserved semen characteristics of Holstein bulls for different preservation periods (cooling at 5°C, 48h, 1, 2 and 3 months post cryopreservation, PC). Seven Holstein bulls of 2.5-3 years of age were used in this experiment. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7 week experimental period. Pooled semen was equally divided into three treatments using Tris extender. Combinations of vitamin C (2.5mM) were added with either catalase (100IU/ml, T2) or reduced glutathione (2mM, T3) to Tris extender and comparisons in response were made with the control group (Tris extender, T1). Individual sperm motility (IM), viability (V), plasma membrane integrity (PMI), and acrosome integrity (AI) were assessed during all periods of the study along with Malondialdehyde (MDA) concentrations and freezing ability. The IM was greater (P≤0.01) in the T2as compared with the T1 group at all periods of the study. Furthermore, the IM were greater (P≤0.01) in the T3as compared with the T1 group at the 48h time period and at 3 months PC. The V, PMI and AI were greater (P≤0.01) in T2 and T3as compared with the T1 group at all the experimental periods. The MDA was greater (P≤0.01) in the T2as compared with the T1 group at 3 months PC. In conclusion, there was improved semen quality if semen of Holstein bulls was collected and stored in combinations of vitamin C with either catalase (T2) or reduced glutathione (T3) being added to Tris extender. PMID:26861956

  10. TEMPORARY STORAGE OF BOVINE SEMEN CRYOPRESERVED IN LIQUID NITROGEN ON DRY ICE AND REFREEZING OF FROZEN-THAWED SEMEN.

    Science.gov (United States)

    Abdussamad, A M; Gauly, M; Holtz, W

    2015-01-01

    Two experiments were conducted. The purpose of Experiment 1 was to investigate whether viability of bovine semen stored in liquid nitrogen (-196°C) will be adversely affected by temporary exposure to dry ice (-79°C). It was convincingly shown that post thaw-motility was not affected, regardless whether semen was thawed immediately or after being returned to liquid nitrogen. Shipping or temporary storage on dry ice, thus, is a viable option. In Experiment 2, refreezing of frozen-thawed semen was attempted. The proportion of motile spermatozoa was reduced by a factor of ten to between 6.0 % and 7.4 %, regardless whether thawing occurred directly after removal from liquid nitrogen or after an interim period on dry ice. When semen was refrozen on dry ice before being returned to liquid nitrogen, motility rates were significantly improved (13.0 % to 17.0 %, P<0.05). In both experiments sperm cells that remained motile displayed vigorous forward movement and normal morphological appearance. PMID:26576003

  11. Infecciones de transmisión sexual, calidad del semen e infertilidad Sexually transmitted diseases, quality of semen, and infertility

    Directory of Open Access Journals (Sweden)

    Bertha Victoria Rodríguez Pendás

    2008-12-01

    Full Text Available El propósito de este trabajo es insistir en la asociación que existe entre el incremento de las infecciones de transmisión sexual y la infertilidad masculina. Se muestran numerosas investigaciones realizadas en este campo que demuestran el rol de estas infecciones en la etiología de la infertilidad, y se describen algunas de las principales infecciones en el semen que provocan la declinación de la fertilidad masculina y sus consecuencias en la salud reproductiva de los hombres. Con este trabajo de revisión nos proponemos resaltar la necesidad de incluir en el estudio de la infertilidad masculina el control microbiológico del semen, particularmente útil en los servicios de salud reproductiva, donde el riesgo de prevalencia de infecciones asociadas a la infertilidad provoca una reproducción fallida con consecuencias emocionales y sociales en la parejaAim of this paper is to insist on association between the sexually transmitted diseases increase and male infertility. We present most researches performed in this field emphasizing the role of these infections in infertility origin, and we describe also some of main semen infections causing decrease of male fertility, and its consequences on reproductive health of men. Aim of this review paper is to highlight the need of to include in male infertility study, the metabolic control of semen, where risk of infections prevalence associated to infertility provokes a failure reproduction with emotional and social consequences in couple

  12. Study on the effect of prostaglandin F2α treatment on semen characteristics and enzymatic activates of Awassi rams in breeding and non breeding seasons

    Directory of Open Access Journals (Sweden)

    Osama Ibrahim Azawi,

    2011-05-01

    Full Text Available The purpose of this research work was to determine the effects of PGF2α, given immediately before semen collection, on semen characteristics and libido in Awassi rams during breeding and non breeding season. The experiment was conducted in late summer to early autumn when major breeding activities commence and winter during the non breeding season at Mosul region in northern Iraq at the Animal Research and Practice Farm of the College of The Veterinary Medicine, University of Mosul. Twelve mature Awassi rams were used in this study. Animals were randomly allocated into two equal groups, the first group was administered 7.5 mg IM of PGF2αweekly and the second group as a control group received 1 ml of N-saline solution. Semen samples were collected from the Awassi rams 24 h after IM administration. Scrotal circumference (SC and testicular volume were measured weekly during the study period. Semen ejaculates were evaluated for semen volume, sperm concentration, sperm concentration/ejaculate, mass motility, individual motility, percentage live sperm, sperm abnormalities, and sperm acrosomal defects. Samples of seminal plasma were analyzed for the estimation of alanine amino transferase (ALT, aspartate amino transferase (AST, acid phosphatase (ACP, alkaline phosphatase (ALP and lactic dehydrogenase (LDH. Results of the present showed that PGF2α treatment to Awassi rams did not improve most semen characteristics in both breeding and non breeding seasons compared with the group. The only improvement of Awassi semen quality observed was in sperm concentration in the breeding season. The testicular volume showed a significant increase (P<0.05 in Awassi rams treated with PGF2α in breeding season compared to the control group and PGF2α treated group in the non breeding season. The mean activity of LDH enzyme estimated in the PGF2αtreated group and control group showed a significant difference (P<0.05 between the two groups in the breeding season and non breeding season (52.34 ± 8.96 and 57.43 ± 19.9 vs. 117.02 ± 5.26 and 131.88 ± 5.01, respectively. Other enzymatic activities including ALT, AST, ACP and ALP showed no significant differences between Awassi rams treated with PGF2α and control groups in both breeding and non breeding seasons. In conclusion, PGF2αtreatment of Awassi rams improved sperm concentration and testicular volume

  13. Air pollution and decreased semen quality: A comparative study of Chongqing urban and rural areas

    International Nuclear Information System (INIS)

    To investigate the association and effects of air pollution level on male semen quality in urban and rural areas, this study examines the outdoor concentrations of particulate matter (PM10), sulfur dioxide (SO2), nitrous dioxide (NO2) and semen quality outcomes for 1346 volunteers in both urban and rural areas in Chongqing, China. We found the urban area has a higher pollution level than the rural area, contrasted with better semen quality in the rural residents, especially for sperm morphology and computer assistant semen analysis (CASA) motility parameters. A multivariate linear regression analysis demonstrates that concentrations of PM10, SO2, and NO2 significantly and negatively are associated with normal sperm morphology percentage (P 10, SO2, and NO2 in urban ambient air may account for worse semen quality in urban males. - Highlights: • We investigate the distributions of PM10, SO2 and NO2 in urban and rural areas in Chongqing, China. • We explore the associations of air pollution and male semen quality. • The concentrations of PM10, SO2, and NO2 are significantly higher in urban areas. • Median values of some semen quality parameters in rural male were higher than urban male. • PM10, SO2, and NO2 were negatively associated with semen quality parameters. - Air pollution is higher in the urban area while there is better semen quality in rural males. Polluted air may thus account for worse semen quality in urban males

  14. Functional characterisation of semen in honeybee queen (A.m.ligustica S. spermatheca and efficiency of the diluted semen technique in instrumental insemination

    Directory of Open Access Journals (Sweden)

    Andrea Galli

    2010-01-01

    Full Text Available Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica queen spermatheca werestudied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05 between the 12th and 24th month.The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminatedtwice with a Hyes solution/semen mixture (1:1 stored as many spermatozoa in their spermatheca as those inseminatedonce with the classic technique. Queen replacement, oviposition and other functional characteristics were similarto those observed in the classic insemination procedure.

  15. Studies on Freezing RAM Semen in Absence of Glycerol.

    Science.gov (United States)

    Abdelnaby, Abdelhady Abdelhakeam

    1988-12-01

    Glycerol is widely used as a major cryoprotective agent for freezing spermatozoa of almost all species. However, it reduces fertility of sheep inseminated cervically compared with intrauterine insemination. Studies were conducted to develop a method and procedure for freezing ram semen in the absence of glycerol. Post -thaw survival of ram spermatozoa frozen in the absence of glycerol was affected by time and temperature after collection and before dilution and time after dilution and before freezing. Increase in time at 5^ circC before or after dilution and before freezing increased both post-thaw motility and number of cells passing through Sephadex filter. A cold dilution method was developed. Slow cooling of fresh ram semen and diluting at 5^circ C 2-3 hr. after collection, then freezing 1 hr. after dilution improved both post-thaw motility and number of cells passing through Sephadex filter compared with immediate dilution at 30-37^circC after collection and freezing 3-4 hr. later (P semen in the absence of glycerol. An increase in post-thaw motility was obtained when semen was extended in TES titrated with Tris to pH 7.0 (TEST) and osmotic pressure of 375-400 mOsm/kg, containing 25-30% (v/v) egg yolk and 10% (v/v) maltose. A special device (boat) for freezing was constructed to insure the same height of the sample above LN _2 and thus the same freezing rate from freeze to freeze. Freezing of semen in 0.25cc straws at 5-10 cm above LN_2 (73.8 to 49.5 ^circC/min) yielded higher post-thaw motility than the rates resulted from freezing at 15 cm above LN_2 or 1 cm above LN _2. Faster Thawing in 37^ circC water for 30 sec. (7.8^ circC/sec.) increased post-thaw motility compared with slower thawing in 5 or 20^circ C water (P semen frozen without glycerol and 17.1% in a second trial. One injection (IM) of 15 mg PGF_{2alpha}/ewe for estrus synchronization during breeding season resulted in higher heat response and lambing rate than two injections given 10 days apart.

  16. Genetic gain in dairy cattle populations is increased using sexed semen in commercial herds

    DEFF Research Database (Denmark)

    Sørensen, Morten Kargo; Andersen, Jakob Voergaard; Pedersen, Louise Dybdahl; Berg, Peer; Sørensen, Anders Christian

    2011-01-01

    100 cows each. Each year 50 young bulls (YB), 10 active sires and 215 BD were selected on best linear unbiased prediction estimated breeding values by truncation selection across the simulated population, and the YB were tested within the population. Use of sexed semen alone gave a positive increase......Using stochastic simulation, the effect of using sexed semen to cow dams (CD) in a dairy cattle breeding scheme, with or without use of multiple ovulation and embryo transfer (MOET) to bull dams (BD), on annual genetic gain at the population level was examined. Three levels of sexed semen were...... combined with three levels of MOET: no sexed semen, sexed semen to the best CD and sexed semen to all heifers, combined with no MOET, MOET on all BD and MOET randomly on 20% of the BD. In total, nine scenarios were compared. The simulated population was monitored for 30 years and included 450 herds with...

  17. Semen Characteristics of Three Strains of Local Cocks in the Humid Tropical Environment of Nigeria

    Directory of Open Access Journals (Sweden)

    F.O. Ajayi

    2011-06-01

    Full Text Available The study was conducted to determine the semen characteristics of three genotypes of Nigerian indigenous cocks. Thirty Six (36 local breeding cocks comprising of 12 frizzle, 12 normal and 12 naked neck selected randomly from the poultry breeding unit of the University of Port Harcourt Teaching and Research farm was used for this study. Semen were collected from them by abdominal massage and analyzed for semen characteristics. Semen concentration were significantly higher in naked- neck 4.86×109 ±0.03/mL (p0.05 of strains on semen pH, abnormal sperm and non-motile sperm. Morphological defects of the head, middle and tail was not significantly affected (p>0.05 by the genotypes. Variations on semen characteristics abound in the three Nigerian indigenous cocks sampled.

  18. Efecto de dos dilutores sobre la motilidad e integridad de la membrana espermtica en semen congelado de ovinos / Effects of two semen extenders on motility and integrity of sperm membrane in ovine frozen semen

    Scientific Electronic Library Online (English)

    Prspero, Cabrera V; Javier, Orellana Ch; Csar, Pantoja A.

    2010-07-01

    Full Text Available El presente estudio tuvo como objetivo evaluar el efecto de dos dilutores, Tris- Fructosa-Yema de huevo (Tris) y Citrato-Glucosa-Yema de huevo (citrato), sobre la motilidad espermtica e integridad de la membrana espermtica (HOST) en semen congelado de ovinos bajo la forma de pellets. La investigac [...] in se llev a cabo en el Banco de Semen de la Universidad Nacional Agraria La Molina, Lima, emplendose 4 carneros (2 Blackbelly y 2 Assaf) de 3.5 a 4 aos de edad. Se emple el anlisis de covariancia para analizar Motilidad Individual Progresiva (MIP), y bloques completamente randomizados para medir el efecto de los dilutores sobre la integridad de la membrana espermtica. Para el congelamiento del semen se utiliz hielo seco y el descongelamiento se realiz a 38 C en tubos de ensayo. En ovinos Assaf, la MIP del semen descongelado fue de 63.77 y 61.11% utilizando Tris y citrato, respectivamente, encontrndose diferencias significativas (p Abstract in english The objective of the study was to evaluate the effects of two semen extenders: Tris- Fructose-egg yolk (Tris) and Citrate-Glucose-egg yolk (citrate) on motility and sperm membrane integrity (HOST) in ovine frozen semen in pellets. The study was carried out at the Semen Bank of the Agrarian Universit [...] y La Molina, in Lima, Peru, using 4 rams (2 Assaf and 2 Blackbelly) of 3.5 to 4 years old. A covariance analysis was used to evaluate the effect of the treatment and breed on Individual Progressive Motility (IPM), and randomized block design to evaluate the effect of extenders on sperm membrane integrity. Semen was frozen of dry ice and thawing was done in test tubes at 38 C. In the Assaf breed, IPM of thawed semen was 63.77 and 61.11% when using Tris and citrate respectively, showing statistical difference (p

  19. Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

    OpenAIRE

    Daniella A. J. Paula; Estefânia S. Andrade; Luis D. S. Murgas; Viviane O. Felizardo; Elissandra U. Winkaler; Walmes Zeviani; Rilke T. F. Freitas

    2012-01-01

    This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus) semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga-MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7) with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing...

  20. Influence of PGF2a on semen quality and libido in Holstein bulls

    OpenAIRE

    MASOUMI, Reza; Towhidi, Armin; JAVAREMI, Ardsher N.; NABIZADEH, Habib; ZHANDI, Mehdi

    2011-01-01

    The aim of this study was to determine the effects of Cloprostenol (PGF2a analogue) on semen quality and libido in Holstein bulls. Ten low libido Iranian Holstein bulls were randomly allocated to 2 groups and received Cloprostenol (n = 5) or saline (n = 5) 30 min prior to the semen collection 2 days per week for 2 months. Reaction time was significantly decreased in the treatment group. Duration of ejaculation was significantly increased in the treatment group. Semen volume and sperm conc...

  1. Kualitas Semen Beku Kuda pada Pengencer Susu Skim dengan Konsentrasi Gliserol yang Berbeda

    OpenAIRE

    Azizah -; Raden Iis Arifiantini

    2009-01-01

    Cryoprotectant is one of determining factors for the success of stallion semen cryopreservation. Formost mammalian sperm cryopreservation,glycerol has been widely used as the cryoprotectant. Glycerol inhigh concentration is toxic to the sperm but in low concentration it has protective effect on the semen. Theobjectives of this experiment were to find appropiate glycerol concentration by using skim milk extender inorder to maintain motility and viability of spermatozoa of stallion frozen semen...

  2. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study

    OpenAIRE

    Khairiah, M.S.; I. Zawawi; H. Wahid; Hajarian, H.; Fahrul, F.J.; Hafiz, M.D.; Hafiz, M.M.; Z.F. Ann; M.I. Iswadi; O. A. Mazni

    2012-01-01

    The Malayan gaur (Bos gaurus hubbacki) or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN). The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM) and electroejaculation (EEJ) technique was applied in semen collection of the Malayan gaur. The semen w...

  3. A novel method for semen collection and artificial insemination in large parrots (Psittaciformes)

    OpenAIRE

    Michael Lierz; Matthias Reinschmidt; Heiner Mller; Michael Wink; Daniel Neumann

    2013-01-01

    The paper described a novel technique for semen collection in large psittacines (patent pending), a procedure which was not routinely possible before. For the first time, a large set of semen samples is now available for analysis as well as for artificial insemination. Semen samples of more than 100 psittacine taxa were collected and analysed; data demonstrate large differences in the spermatological parameters between families, indicating an ecological relationship with breeding behaviour (p...

  4. Influence of Deficiency or Supplementary Selenium and a- Tochopherol (Vitamin E) In The Diet of Pubertal Male Zaraibi Goats on Fertility, Semen Quality and Testicular Traits

    International Nuclear Information System (INIS)

    Twenty pubertal male Zaraibi goats (bucks) were randomly divided into four equal groups; fed deficient Se or vit. E, adequate Se, adequate vit. E and adequate Se + vit. E diets for 3 months to study the influence of deficient or adequate selenium (Se) and vitamin E (vit. E) in the diet of pubertal male Zaraibi goats on fertility, semen quantity and quality and some testicular traits. The results showed that the best values of semen quantity (the ejaculate volume, sperm concentration and total sperm output per ejaculate) and semen quality (percentage of progressive motility, percentage of live sperm, number of motile sperm per ejaculate, percentage of dead, abnormal spermatozoa and acrosomal abnormality) were observed in bucks fed diet supplemented with adequate Se combined with adequate vit. E. The lowest values of semen quantity and semen quality were observed in bucks suffering from deficiency of Se and/or vit. E in their diets. Testosterone level in seminal plasma was significantly higher in bucks fed adequate Se and/or vit. E than those fed diet deficient in Se and vit. E. Testosterone level was significantly higher in bucks fed diet adequate in Se + vit. E than those fed diet adequate with Se or vit. E alone. Se and vit. E deficiency in the diets was accompanied by a significant decrease in testosterone, T4 and T3 levels in seminal plasma. Selenium or vit. E each one alone supplementation led to increases of these hormones. T4 and T3 levels were significantly higher in bucks fed adequate Se or adequate Se + vit. E than in bucks fed diet with adequate vitamin E alone. Adequate Se alone and adequate Se + vit. E diets were accompanied by significant increases in Se in seminal plasma. Adequate vit. E and adequate Se + vit. E diets were accompanied by significant increase in vit. E level in the seminal plasma. It is clear that there was synergism between Se and vit. E in the biological role of Se, since the level of Se in bucks fed diet containing adequate Se + vit. E was higher than the level of Se in group fed Se alone. The highest values of scrotal circumference and scrotum length were observed in bucks fed adequate Se + vit. E and the lowest testicular traits and fertility were observed in bucks fed diet deficient with Se and vit. E.

  5. Symmetry-breaking phase-transitions in highly concentrated semen

    Science.gov (United States)

    Plouraboué, Franck; Creppy, Adama; Praud, Olivier; Druart, Xavier; Cazin, Sébastien; Yu, Hui; Degond, Pierre

    2015-11-01

    New experimental evidence of self-motion of a confined active suspension is presented. Depositing fresh semen sample in an annular shaped micro-fluidic chip leads to a spontaneous rotation motion of the fluid at sufficiently large sperm concentration. The rotation occurs unpredictably clockwise or counterclockwise and is robust and stable. Furthermore, for highly active and concentrated semen, richer dynamics can occur such as self-sustained or damped rotation oscillations. Experimental results obtained with systematic dilution provide a clear evidence of a phase transition toward collective motion associated with local alignment of spermatozoa akin to the Vicsek model. A macroscopic theory based on previously derived Self-Organized Hydrodynamics (SOH) models is adapted to this context and provides predictions consistent with the observed stationary motion.

  6. Association of Sleep Disturbances With Reduced Semen Quality

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Andersson, Anna-Maria; Skakkebæk, Niels Erik; Joensen, Ulla Nordstrøm; Jensen, Martin Blomberg; Lassen, Tina Harmer; Nordkap, Loa; Olesen, Inge Alhmann; Hansen, Åse Marie; Rod, Naja Hulvej; Jørgensen, Niels

    2013-01-01

    Several studies have found an association between sleep duration and morbidity and mortality, but no previous studies have examined the association between sleep disturbances and semen quality. We conducted a cross-sectional study among 953 young Danish men from the general population who were...... recruited in Copenhagen at the time of determination of fitness for military service between January 2008 and June 2011. All of the men delivered a semen sample, had a blood sample drawn, underwent a physical examination, and answered a questionnaire including information about sleep disturbances. Sleep...... disturbances were assessed on the basis of a modified 4-item version of the Karolinska Sleep Questionnaire, which includes questions on sleep patterns during the past 4 weeks. Sleep disturbances showed an inverse U-shaped association with sperm concentration, total sperm count, percent motile and percent...

  7. Semen collection and evaluation of captive coatis (Nasua nasua

    Directory of Open Access Journals (Sweden)

    R.C.R. Paz

    2012-04-01

    Full Text Available Semen samples (n=105 were collected through eletroejaculation from six adult male coatis (Nasua nasua between January 2007 and December 2008 at Universidade Federal de Mato Grosso Zoo, Cuiabá, Brazil. Mean values were: volume (mL; concentration (sperm/mL; total motility (%; progressive sperm motility (scale, 0-5; live spermatozoa (%; acrossome integrity (%; primary defects (%; and secondary defects (%. There was high correlation between total motility and live sperm; total motility and progressive sperm motility; total motility and acrossome integrity; live sperm and progressive motility; live sperm and acrossome integrity and volume and concentration. The method for semen collection was considered safe and efficient. It can be used for the evaluation of breeding potential of coati in captivity and for the establishment of new assisted reproductive technology (ART for threatened neotropical carnivores species.

  8. Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status.

    Science.gov (United States)

    Hamilton, Thais Rose Dos Santos; Castro, Letícia Signori de; Delgado, Juliana de Carvalho; de Assis, Patrícia Monken; Siqueira, Adriano Felipe Perez; Mendes, Camilla Mota; Goissis, Marcelo Demarchi; Muiño-Blanco, Teresa; Cebrián-Pérez, José Álvaro; Nichi, Marcílio; Visintin, José Antonio; D'Ávila Assumpção, Mayra Elena Ortiz

    2016-04-01

    Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm. PMID:26811546

  9. Effect of L-carnitine supplementation on drake semen quality

    Scientific Electronic Library Online (English)

    H.J., Al-Daraji; A.O., Tahir.

    2014-01-01

    Full Text Available This study was conducted to determine the effect on semen quality traits of supplementing the diets of Iraqi drakes with L-carnitine. Forty eight male Iraqi ducks, 30 weeks old, were randomly allocated to four treatments with 12 drakes per treatment group, replicated three times, with four drakes pe [...] r replicate. The treatment groups consisted of birds fed a diet free of L-carnitine (T1, control group); birds fed a diet containing 50 mg L-carnitine/kg diet (T2); birds fed a diet containing 100 mg L-carnitine/kg diet (T3); and birds fed a diet containing 150 mg L-carnitine/kg diet. The drakes were fed the experimental diets only during the experimental period, which lasted three months. The semen quality traits that were investigated were ejaculate volume, mass and individual motility of spermatozoa, spermatocrit, spermatozoa concentration, percentages of dead and abnormal spermatozoa and acrosomal abnormalities. Supplementing the diet of drakes with L-carnitine at the levels of 50 - 150 mg/kg diet significantly increased ejaculate volume, spermatocrit, mass and individual motility of spermatozoa, and concentration of spermatozoa, while percentages of dead and abnormal spermatozoa and acrosomal abnormalities were decreased. However, T4 (150 mg L-carnitine/kg diet) recorded the best results in relation to all semen quality traits included in this study. Dietary supplementation with L-carnitine improved the semen quality of local drakes; therefore L-carnitine can be used as an efficient feed additive to improve the reproductive performance of male ducks.

  10. Analisa Pengelolaan Modal Kerja pada Perusahaan Industri Semen

    OpenAIRE

    Engelwati Gani

    2011-01-01

    The purpose of this research is to identify financial ratios, to analyse the relation among the financial ratios, and to compare financial ratios of PT Indocement Tunggal Prakarsa, Tbk., PT Semen Gresik (Persero), Tbk., dan PT Holcim Indonesia, Tbk. The research method is qualitative, descriptive research, with the research time dimension using time series. The research depth is using the three industrial cement companies that listed in Indonesia Stock Exchange (BEJ). The gathering data metho...

  11. Effects of age, season and genetics on semen and sperm production in Apis mellifera drones

    OpenAIRE

    Rhodes, John; Harden, Steven; Spooner-Hart, Robert; Anderson, Denis; Wheen, Gretchen

    2011-01-01

    Adult drone honey bees from 4 Australian breeding lines were reared under similar conditions and examined for semen and sperm production when 14, 21 and 35 days old, during spring, summer and autumn. Almost half (40.5%) of all drones examined did not release any semen when manually everted. For those that released semen, the average volume released per drone was 1.09 μL (range 0.72 (±0.04)−1.12 (±0.04) μL) and the average number of sperms in the semen per drone was 3.63 × 106 (range 1.88 (±0....

  12. Decreases in Human Semen Quality with Age Among Healthy Men

    Energy Technology Data Exchange (ETDEWEB)

    Eskenazi, B.; Wyrobek, A.J.; Kidd, S.A.; Moore, L.; Young, S.S.; Moore, D.

    2001-12-01

    The objective of this report is to characterize the associations between age and semen quality among healthy active men after controlling for identified covariates. Ninety-seven healthy, nonsmoking men between 22 and 80 years without known fertility problems who worked for or retired from a large research laboratory. There was a gradual decrease in all semen parameters from 22-80 years of age. After adjusting for covariates, volume decreased 0.03 ml per year (p = 0.001); sperm concentration decreased 2.5% per year (p = 0.005); total count decreased 3.6% per year of age (p < 0.001); motility decreased 0.7% per year (P < 0.001); progressive motility decreased 3.1% per year (p < 0.001); and total progressively motile sperm decreased 4.8% per year (p < 0.001). In a group of healthy active men, semen volume, sperm concentration, total sperm count, and sperm motility decrease continuously between 22-80 years of age, with no evidence of a threshold.

  13. Glyph-Based Video Visualization for Semen Analysis

    KAUST Repository

    Duffy, Brian

    2015-08-01

    © 2013 IEEE. The existing efforts in computer assisted semen analysis have been focused on high speed imaging and automated image analysis of sperm motility. This results in a large amount of data, and it is extremely challenging for both clinical scientists and researchers to interpret, compare and correlate the multidimensional and time-varying measurements captured from video data. In this work, we use glyphs to encode a collection of numerical measurements taken at a regular interval and to summarize spatio-temporal motion characteristics using static visual representations. The design of the glyphs addresses the needs for (a) encoding some 20 variables using separable visual channels, (b) supporting scientific observation of the interrelationships between different measurements and comparison between different sperm cells and their flagella, and (c) facilitating the learning of the encoding scheme by making use of appropriate visual abstractions and metaphors. As a case study, we focus this work on video visualization for computer-aided semen analysis, which has a broad impact on both biological sciences and medical healthcare. We demonstrate that glyph-based visualization can serve as a means of external memorization of video data as well as an overview of a large set of spatiotemporal measurements. It enables domain scientists to make scientific observation in a cost-effective manner by reducing the burden of viewing videos repeatedly, while providing them with a new visual representation for conveying semen statistics.

  14. Prospective surveillance of semen quality in the workplace

    Energy Technology Data Exchange (ETDEWEB)

    Schenker, M.B.; Samuels, S.J.; Perkins, C.; Lewis, E.L.; Katz, D.F.; Overstreet, J.W.

    1988-04-01

    We performed a prospective surveillance of semen quality among workers in the plant where 1,2-dibromo-3-chloropropane was first recognized as an occupational cause of impaired semen quality and of infertility. All male employees of the Agricultural Chemical Division were required to participate. Ninety-seven workers (92% participation) provided 258 semen samples over the 4 years of the program. Most samples were analyzed at the plant with a mini-laboratory designed for the study. Motility and shape measures were made objectively. Sixty-six subjects (68%) were non-azoospermic. Generalized multiple regression showed no significant predictors for any response, with the exception of the motility measures, which were reduced with longer times between ejaculation and assay. Between- and within-person standard deviations and correlations were calculated. Comparison of this population with fertile artificial insemination donors (16 men, 498 ejaculates) revealed generally higher ejaculate-to-ejaculate standard deviations in the worker samples. This is probably due to less well controlled conditions of sperm collection in the workplace setting. For cross-sectional studies, one ejaculate per worker is recommended as sufficient; for estimating an individual worker's mean, even three ejaculates may not provide enough precision.

  15. Biochemical properties of microbial load in frozen semen of cattle

    Directory of Open Access Journals (Sweden)

    Hemaxi V Patel

    2011-06-01

    Full Text Available A total 20 French mini straws (0.25ml of frozen semen were randomly collected from one of frozen semen bank for evaluation of microbial load using the standard plate count (SPC method using nutrient agar plate. These plates were incubated at 37oC for 24 and 48 hrs and examined for growth. The average colony count was calculated and bacteria were also identified as Gram positive and Gram negative. A total of 10 biochemical tests were performed to characterize the isolates. Antibiotic sensitivity test was also performed to test the sensitivity against Ampicillin, Erythromycin, Gentamycin, Spectinomycin and Tetracycline. The results indicate that 5 samples out of 20 were found positive for various bacterial isolates and fungi. Both the Gram positive and Gram negative bacteria were found in these samples. One isolate from sample No. CB-594 (white colony was found positive for all 9 biochemical tests. All the bacterial isolates exhibited variable pattern against 5 antibiotics used in the study. The article describes detailed investigation of microbial load in frozen semen of cattle bulls.

  16. Persistence of DNA from laundered semen stains: Implications for child sex trafficking cases.

    Science.gov (United States)

    Brayley-Morris, Helen; Sorrell, Amber; Revoir, Andrew P; Meakin, Georgina E; Court, Denise Syndercombe; Morgan, Ruth M

    2015-11-01

    In sexual assault cases, particularly those involving internal child sex trafficking (ICST), victims often hide their semen-stained clothing. This can result in a lag time of several months before the items are laundered and subsequently seized during a criminal investigation. Although it has been demonstrated previously that DNA can be recovered from clothing washed immediately after semen deposition, laundered items of clothing are not routinely examined in ICST cases, due to the assumption that the time delay and washing would result in no detectable DNA. The aim of this study was to examine whether viable DNA profiles could be recovered from laundered semen stains where there has been a significant lag time between semen deposition from one or more individuals and one or more washes of the stained clothing. Items of UK school uniform (T-shirts, trousers, tights) were stained with fresh semen (either from a single donor or a 1:1 mixture from two donors) and stored in a wardrobe for eight months. Stained and unstained items (socks) were then washed at 30 °C or 60 °C and with non-biological or biological detergent. DNA samples extracted from the semen-stained sites and from the unstained socks were quantified and profiled. High quantities of DNA, (6-18 μg) matching the DNA profiles of the semen donors, were recovered from all semen-stained clothing that had been laundered once, irrespective of wash conditions. This quantity,and profile quality,did not decline significantly with multiple washes. The two donor semen samples yielded ∼ 10-fold more DNA from the T-shirts than from the trousers. This disparity resulted in the T-shirts yielding a ∼ 1:1 mixture of DNA from the two donors, whereas the trousers yielded a major DNA profile matching only that of the second donor. The quantities of DNA recovered from the unstained socks were an order of magnitude lower, with most of the DNA being attributable to the donor of the semen on the stained clothing within the same wash, demonstrating the transfer of semen-derived DNA among items of clothing in the washing machine. This study demonstrates that complete DNA profiles can be obtained from laundered semen stains on school uniform-type clothing, with an eight-month lag time between semen deposition and laundering, despite multiple washes and stains from two semen donors. These data emphasise the need to recover and examine the clothing of victims for semen and DNA evidence, even if the clothing has been stored for several months or washed multiple times since the sexual offence took place. PMID:26232275

  17. Effect of glycerol on the viability and fertility of cooled bovine semen.

    Science.gov (United States)

    Papa, Patricia M

    2015-01-01

    The aim of the present study was to compare the viability and fertility of bovine semen diluted in Botu-Bov (BB) commercial extender with and without the cryoprotectant glycerol then cooled at 5 degree C for 24 hours in the Botu-Flex passive cooling system and of semen diluted in BB with glycerol then frozen. One ejaculate of 30 Nelore Bos Taurus indicus bulls between 24 and 30 months of age was used for in vitro analysis. Sperm kinetics and cell viability were analyzed using computer-assisted sperm analysis and flow cytometry, respectively. Three Nelore bulls approximately 30 month old were used for in vivo test using fixed-time artificial insemination for the fertility analysis. The ejaculates were divided into three experimental groups: semen in BB extender with 7% glycerol cooled at 5 °C for 24 hours (cooled semen with cryoprotectant), semen in BB without glycerol cooled at 5 °C for 24 hours (cooled semen without cryoprotectant), and semen diluted in BB with 7% glycerol then subsequently frozen rather than cooled (frozen semen). For the fertility analysis, 762 Nelore cows (B taurus indicus) were randomly inseminated using fixed-time artificial insemination. For the groups corresponding to cooled semen with cryoprotectant, cooled semen without cryoprotectant, and frozen semen, 278, 268, and 216 cows were inseminated, respectively, and the resulting conception rates were 51% a, 44%ab and 41%b (P < 0.05), respectively. In conclusion, the fertility rates improved, when samples were cooled with glycerol at 5 °C for 24 hours compared with the frozen samples. PMID:25441498

  18. Semen Quality of Holstein and Buffalo Bulls after Filtration using Sephadex Column

    International Nuclear Information System (INIS)

    To evaluate the effect of sephadex column filtration technique on semen quality of five Holstein bulls and five Egyptian buffalo bulls. Semen was collected biweekly from each eight weeks. Immediately after collection, semen was extended (37degree C) and filtered using sephadex column-filtration technique. Semen was evaluated for physical semen characteristics including, percentages of sperm motility, live sperm and sperm abnormality as well as sperm cell concentration pre-and post-filtration. Results show that among all physical semen characteristics, only ejaculate semen volume was significantly (P<0.001) higher in Holstein than buffalo bulls, but motility, livability, abnormality, sperm concentration and sperm with intact acrosome did not differ between both species. As a result of filtration, sperm motility and livability increased (P<0.05) by 16.4 and 11.8% in Holstein and by 16.9 and 10.1% in buffalo semen, respectively. Sperm abnormality and concentration reduced (P<0.05) by 2.6 and 3.3% in Holstein and by 2.4 and 3.5% in buffalo semen, respectively. Improvements of live sperm and the reduction in sperm concentration (proportional to the pre-filtration value) were better (P<0.05) in Holstein than buffalo semen (15.5% and %52.4 vs. 13.2 and -49.3%, respectively). Improvement of motility and abnormality did not differ in Holstein (25.4 and %57.8) and buffalo semen (26.6 and ,(%54.5respectively. The present results indicate that using sephadex column filter technique has beneficial effects on improving quality of spermatozoa in both species. (author)

  19. Flora bacteriana del semen de toro antes y después de la congelación (Bacterial flora of bull semen before and after freezing process

    Directory of Open Access Journals (Sweden)

    Enrique A. Silveira Prado

    2005-10-01

    Full Text Available Se investigó por bacteriología general el semen fresco y después de la congelación de 50 toros de inseminación artificial y se efectuó el conteo total de unidades formadoras de colonias (UFC. A l5 de los toros se les realizó el examen bacteriológico de sus lavados prepuciales. En todas las muestras de semen fresco se obtuvo crecimiento bacteriano y los gérmenes más frecuentemente aislados fueron: Escherichia coli (50,0%, Staphylococcus aureus (36,0% y Staphylococcus coagulasa negativa (28,0%. En el semen congelado solamente se obtuvo crecimiento en el 20,0%. El 74,0% del semen fresco alcanzó conteos  1 x 104 UFC/mL antes de ser procesado; después de la congelación el 80,0% fue estéril. En el total de lavados prepuciales se obtuvo crecimiento y se detectó en mayor proporción el Staphylococcus coagulasa negativa (60,0%, microorganismo también aislado en el semen fresco de estos toros. Se concluyó que la adición de antibióticos al menstruo y posterior congelación en pastillas, disminuye notablemente la carga microbiana presente en el semen. It was investigated through general bacteriology both fresh semen and after the freezing process, carried out in 50 bulls of artificial insemination, total counting of colony forming units (CFU was made. A bacteriological analysis of the prepucial washing was made on 15 of these bulls. In all samples of fresh semen there was bacterial growing. The most frequently germs were: Escherichia coli (50,0%, Staphylococcus aureus (36,0% and coagulase negative Staphylococcus (28,0%. In samples of frozen semen growth was only obtained in the 20,0%. The 74,0% of samples of fresh semen reached counts  1 x 104 CFU/mL before being processed; after freezing 80,0% of the samples were sterile. In all prepucial washings it was obtained growth and mostly detected coagulase negative Staphylococcus (60.0%, was also isolated in the fresh semen of these bulls. We concluded that the addition of antibiotics to the menses and later freezing in pills, diminishes the load microbial present notably in the semen

  20. EFFECTS OF A NEW ANTIBIOTIC COMBINATION ON POST -THAW MOTION CHRACTERISTICS AND MEMBRANE INTEGRITY OF BUFFALO AND SAHIWAL BULL SPERMATOZOA AND ON THE BACTERIOLOGICAL QUALITY OF THEIR SEMEN

    Directory of Open Access Journals (Sweden)

    S. Hasan, S. M. H. Andrabi, R. Muneer, M. Anzar and N. Ahmad

    2001-01-01

    Full Text Available In this study the effects of a new antibiotic combination, i.e., gentamycin, tylosin and linco-spectin (STLS on post-thaw motion characteristics, plasma membrane integrity, sperm morphology and the total aerobic bacterial counts (TABC in buffalo and Sahiwal bull semen were investigated. Ten ejaculates, five each from a buffalo and a Sahiwal bull, possessing more than 60% sperm motility were used. These ejaculates were diluted in Tris-citric acid (TCA extender (at 37 C; 50 X 106 spermatozoa/mi, containing either GTLS (gentamycin 500 ?g/ml, tylosin 100 ?g/ml and linco-spectin 300/600 ?g/ml, streptomycin 1000 ?g/ml and penicillin 1000 IU/ml (SP, or negative control with no antibiotics (NCON. Samples were cooled to 4C in 2 hours, equilibrated at 4C for 4 hours, filled in 0.5 ml straws, frozen in a controlled rate cell freezer and plunged into liquid nitrogen. Frozen semen was thawed at 37C for 15 seconds. Post-thaw sperm motion characteristics, plasma membrane integrity and sperm morphology were determined. Total aerobic bacterial counts and the frequency of appearance of bacterial genera were determined in neat semen, after dilution, and after freezing and thawing. Mean motilities (visual; computer-assisted, linear and circular, velocities (straight-line, average path and curvilinear and lateral head displacement (LHD in post- thaw semen samples did not differ due to antibiotics or species. Same was true for sperm plasma membrane integrity. Morphologically abnormal spermatozoa were lower (P<0.05 in GTLS and SP than in NCON. Sperm cells possessing normal acrosomes were higher (P<0.01 in GTLS and SP than in NCON. Total aerobic bacterial counts in post-thaw samples were lower (P<0.05 in GTLS than in SP or NCON. Staphylococcus and micrococcus were lower in samples treated with GTLS than that of SP or NCON. Pseudomonas and E.coli were more frequent in buffaloes than Sahiwal bull samples. Proteus and corynebacteria were scarcely present. In conclusion, GTLS was not determintal to post thaw motion characteristics, sperm morphology and membrane integrity of buffalo and Sahiwal bull spermatozoa. Furthermore, it efficiently reduced the number of aerobic micro-organisms in buffalo and Sahiwal bull semen.

  1. EFFECT OF TWO PROTOCOLS OF CRYOPRESERVATION ON FERTILIZING CAPACITY OF STALLION (Equus caballus SEMEN EFECTO DE DOS PROTOCOLOS DE CRIOPRESERVACIÓN SOBRE LA CAPACIDAD FECUNDANTE DE SEMEN EQUINO (Equus caballus

    Directory of Open Access Journals (Sweden)

    Giovanni Restrepo Betancur

    2012-06-01

    Full Text Available Abstract. Semen cryopreservation is a fundamental process for the development of biotechnologies for assisted reproduction in horses. The use of cryopreservation techniques with changes in concentrations and the nature of the cryoprotectant, as well as, the different types of vials for storage of semen, have become an alternative to improve the protocols used. The objective of this work was to evaluate the effect of two protocols of cryopreservation (freezing and vitrification on the fertilizing capacity of stallion semen. The study was conducted with horses of the Criollo Colombiano breed. For freezing was used a extender supplemented with egg yolk (4% and dimethyl formamide (5%, and 0.5 mL straws as vials, whereas for vitrification, the extender was supplemented with egg yolk (8% and dimethyl formamide (8%, and cryovials were used as carriers. As post thaw parameters were evaluated: progressive motility, vitality, normal morphology and integrity of the plasma membrane through the hypoosmotic swelling test (HOS. For statistical evaluation was fitted a generalized linear model (GLM and means were compared by the Tukey test. Were found average percentages of progressive motility, vitality, normal morphology and HOS of 41.6 ± 11.8 and 37 ± 8.5, 54.3 ± 10.2 and 52.3 ± 7.8, 83.1 ± 5.4 and 83.6 ± 5.8, 41.7 ± 9.8 and 38.9 ± 3.6, for cryopreserved semen by freezing and vitrification, respectively. There were no statistically significant differences (P ≤ 0.05 between treatments for any of the parameters evaluated. The fertilizing capacity of equine semen cryopreserved by vitrification is comparable to that obtained by conventional freezing.Resumen. La criopreservación de semen es un proceso fundamental en el desarrollo de biotecnologías para la reproducción asistida en equinos. El uso de diferentes técnicas de criopreservación con cambios en las concentraciones y la naturaleza de los crioprotectores, así como en los diferentes tipos de soportes para el almacenamiento del semen, se ha constituido en una alternativa para mejorar los protocolos empleados. El objetivo de este trabajo fue evaluar el efecto de dos protocolos de criopreservación (congelación y vitrificación, sobre la capacidad fecundante del semen equino. El estudio se realizó con equinos de la raza Criollo Colombiano. Para la congelación se empleó un diluyente suplementado con de yema de huevo (4% y dimetilformamida (5%, y pajillas de 0,5 mL como soportes; mientras que para la vitrificación, el diluyente fue suplementado con yema de huevo (8% y dimetilformamida (8% y se usaron crioviales como soportes. Post-descongelación, se evaluaron los parámetros: movilidad progresiva, vitalidad, morfología normal e integridad de la membrana plasmática (HOS. Para la evaluación estadística se ajustó un modelo lineal generalizado (GLM y las medias se compararon por la prueba de Tukey. Se encontraron porcentajes promedio de movilidad progresiva, vitalidad, morfología normal y HOS de 41,6±11,8 y 37,0±8,5, 54,3±10,2 y 52,3±7,8, 83,1±5,4 y 83,6±5,8, 41,7±9,8 y 38,9±3,6, para el semen criopreservado por congelación y vitrificación, respectivamente. No se encontraron diferencias estadísticamente significativas (P ≤ 0,05 entre los tratamientos para ninguno de los parámetros evaluados. La capacidad fecundante del semen equino criopreservado por vitrificación es equiparable a la obtenida por congelación convencional.

  2. Effect of sexed-semen use on Holstein conception rate, calf sex, dystocia, and stillbirth in the United States

    Science.gov (United States)

    Most artificial-insemination organizations in the United States now market sex-sorted semen. For 10.8 million US Holstein breedings with conventional semen since January 2006 and 122,705 sexed-semen breedings, data were available from all breedings for conception rate, 12 and 9% of breedings for cal...

  3. Comparison of Holstein service-sire fertility for heifer and cow breedings with conventional and sexed semen.

    Science.gov (United States)

    Sire conception rate (SCR), a service-sire fertility evaluation implemented in August 2008, is based on up to 7 conventional-semen breedings for parities 1 through 5 (Ccow). The same procedure was used to derive SCR for other types of breedings: sexed semen for cows (Scow) and conventional semen and...

  4. EFFECTS OF GENETICS ON QUALITY AND CRYOPRESERVATION OF SEMEN FROM PEDIGREED CHICKEN LAYER LINES

    Science.gov (United States)

    The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in commercial production or preservation of genetic stocks. The objective of the present work was to compare semen quality from 4 pedigreed layer lines (Lines 1, 2, 3, and 4) before and after freezing. A ...

  5. Detection of Ureaplasma spp. in semen samples from sheep in Brazil

    OpenAIRE

    Sandra Batista dos Santos; Orestes Luiz de Souza Neto; Pedro Paulo Feitosa de Albuquerque; André da Rocha Mota; Pomy de Cássia Peixoto Kim; Érica Paes Barreto Xavier de Moraes; Elmiro Rosendo do Nascimento; Rinaldo Aparecido Mota

    2014-01-01

    A study was conducted to verify the presence of mycoplasmas and ureaplasmas DNA in sheep semen samples from the State of Pernambuco. The PCR assay was conducted of according with standard protocols with generic primers. Mollicutes DNA was detected in 26.0% and Ureaplasma spp. in 12.0% of semen samples.

  6. 9 CFR 98.35 - Declaration, health certificate, and other documents for animal semen.

    Science.gov (United States)

    2010-01-01

    ... the United States in accordance with subpart C of 9 CFR part 98. (e) The certificate accompanying sheep or goat semen intended for importation from any part of the world shall, in addition to the... other documents for animal semen. 98.35 Section 98.35 Animals and Animal Products ANIMAL AND...

  7. Detection of Ureaplasma spp. in semen samples from sheep in Brazil

    Directory of Open Access Journals (Sweden)

    Sandra Batista dos Santos

    2013-09-01

    Full Text Available A study was conducted to verify the presence of mycoplasmas and ureaplasmas DNA in sheep semen samples from the State of Pernambuco. The PCR assay was conducted of according with standard protocols with generic primers. Mollicutes DNA was detected in 26.0% and Ureaplasma spp. in 12.0% of semen samples.

  8. Effect of sexed semen on conception rate for Holsteins in the United States

    Science.gov (United States)

    Effect of sexed-semen breedings on conception rate was investigated using US Holstein field data from January 2006 through October 2008. Sexed-semen breeding status was determined by a National Association of Animal Breeders’ 500-series marketing code or by individual breeding information in a cow o...

  9. Environmental mercury exposure, semen quality and reproductive hormones in Greenlandic Inuit and European men

    DEFF Research Database (Denmark)

    Mocevic, Emina; Specht, Ina O; Marott, Jacob Louis; Giwercman, Aleksander; Jönsson, Bo Ag; Toft, Gunnar; Lundh, Thomas; Peter Bonde, Jens

    2013-01-01

    Several animal studies indicate that mercury is a male reproductive toxicant, but human studies are few and contradictory. We examined semen characteristics and serum levels of reproductive hormones in relation to environmental exposure to mercury. Blood and semen samples were collected from 529...

  10. Research on contents of anthraquinones in Cassiae Semen by principal component analysis.

    Science.gov (United States)

    Cao, Li-juan; Miao, Jing; Liu, Jie-xiu; Gao, Wen-yuan; Li, Xia

    2015-07-01

    Cassiae Semen is a common traditional Chinese medicine, and contents of anthraquinones of Cassiae Semen different significantly from area to area. According to Chinese Pharmacopoeia (2010 edition), only contents of aurantio obtusin and chrysophanol were used to evaluate the quality of Cassiae Semen, another data could be added later. Ten batches of Cassiae Semen from different areas were determined, and total anthraquinones, total free anthraquinones and total combined anthraquinones contents were assessed by ultraviolet visible spectrophotometer, contents of aurantio obtusin, rhein, aloe emodin, emodin, chrysophanol and physcion were determined by HPLC. After that, principal components analysis was used to evaluate these data determined previous by dimension reduction analysis. At last, the result suggests that three main components were found out, it shows that content of aloe emodin could be used to evaluate the quality of Cassiae Semen as well as contents of aurantio obtusin and chrysophanol. And Cassiae Semen from Hebei province posseses higher quality than Cassiae Semen from other different areas. All these results can provide a good reference for quality evaluating of Cassiae Semen medicinal materials at a certain extent. PMID:26697683

  11. 19 Beef cattle pregnancy rates following insemination with aged frozen angus semen

    Science.gov (United States)

    Artificial insemination has proven to be a valuable asset to the cattle industry. It is assumed that once good quality semen is frozen in liquid nitrogen it should remain viable indefinitely; however, semen viability has not been systematically evaluated after being stored for several decades. In th...

  12. Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender.

    Science.gov (United States)

    Najafi, Abozar; Kia, Hossein Daghigh; Mohammadi, Hossein; Najafi, Mir Hossein; Zanganeh, Zaynab; Sharafi, Mohsen; Martinez-Pastor, Felipe; Adeldust, Hamideh

    2014-08-01

    The aim of this study was to evaluate the effects of ergothioneine and cysteamine as antioxidant supplements in a soybean lecithin extender for freezing ram semen. Twenty-four ejaculates were collected from four rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and cysteamine or ergothioneine (2, 4, 6 or 8mM). Motility by CASA, viability, plasma membrane functionality (HOS test), total abnormality, lipid peroxidation, glutathione peroxidase (GPx) activity and capacitation status (CTC staining) were assessed after thawing. Using 6mM of either antioxidant improved total motility. Cysteamine at 6mM and ergothioneine at 4 and 6mM improved viability and reduced lipid peroxidation (malondialdehyde concentration). Both antioxidants improved membrane functionality significantly, except at 8mM. Progressive motility, kinematic parameters, GPx activity, capacitation status and sperm abnormalities were not influenced by the antioxidant supplements. In conclusion, cysteamine at 6mM and ergothioneine at 4 or 6mM seem to improve the post-thawing quality of ram semen cryopreserved in a soybean lecithin extender. PMID:24854868

  13. Identity of prostate specific antigen and the semen protein P30 purified by a rapid chromatography technique.

    Science.gov (United States)

    Graves, H C; Kamarei, M; Stamey, T A

    1990-12-01

    Previous investigators have reported the identity of prostate specific antigen (PSA) and the semen protein p30, but data to support these claims have not been published. We report here the rapid and continuous purification of PSA using a simple two column chromatography technique originally developed for purification of p30. Using commercial antisera to PSA and original antisera to p30 for detection, we show that the two glycoproteins have identical purification profiles by this technique which uses cation exchange chromatography and sizing chromatography. The antigens also have identical molecular weights by gel electrophoresis and gel filtration. Furthermore, both antigens correspond to the same prominent protein band in seminal plasma by sodium dodecylsulfate polyacrylamide gel electrophoresis. On commercial immunoassay for PSA, purified p30 gives a calibration curve identical to the commercial PSA kit calibrator (Pros-checkTM PSA Radioimmunoassay, Yang Laboratories). We conclude that PSA and the semen protein p30 are identical and can be easily purified by a rapid continuous technique. PMID:1700160

  14. Does last week's alcohol intake affect semen quality or reproductive hormones?

    DEFF Research Database (Denmark)

    Hansen, Mette Lausten; Thulstrup, A M; Bonde, Jens Peter; Olsen, J; Håkonsen, L B; Ramlau-Hansen, C H

    2012-01-01

    The association between last 5 days of alcohol intake, semen quality and reproductive hormones was estimated in this cross-sectional study among 347 men. Conventional semen characteristics, DNA fragmentation index and reproductive hormones (testosterone, estradiol, sex hormone-binding globulin...... free testosterone was higher at higher alcohol intake during the 5 days preceding semen sampling. In conclusion, alcohol intake was associated with impairment of most semen characteristics but without a coherent dose-response pattern. The study indicates an association between recent alcohol intake and...... a hormonal shift towards higher estradiol/testosterone ratio. The hormonal changes observed may over time, lead to adverse effects on semen quality, but longitudinal studies are needed to study this....

  15. Obtencin de cachorros mediante inseminacin artificial con semen canino refrigerado.: Primera descripcin en Chile Puppies obtained using artificial insemination with chilled extended semen.: First report in Chile

    OpenAIRE

    SA?NCHEZ, A.; J. RUBILAR

    2001-01-01

    Empleando una pareja de perros Siberian Husky, se describe, por primera vez en Chile, una inseminacin artificial empleando semen canino refrigerado. El semen fue obtenido por manipulacin digital y diluido con leche semidescremada UHT con antibiticos en relacin 1:4 y refrigerado a 5C. Se practicaron 3 inseminaciones a partir del tercer da del estro, el cual fue determinado mediante exmenes de citologa vaginal, considerndose inicio del estro cuando las clulas superficiales consti...

  16. Purification of PSA from human semen

    International Nuclear Information System (INIS)

    Full text: 1. Human seminal plasma collected from many volunteers are pooled and passed through a column of phenyl sepharose equilibrated with 1.25 M ammonium sulphate. Elution is carried out with 1.25 M ammonium sulphate initially, to remove the bulk non-adsorbing proteins. Gradient elution of the absorbed proteins with 0.01 M Tris-HCl, 0.25 M NaCl, pH 7.0 buffer gives a sharp peak containing PSA. At each stage, PSA has to be identified by an independent method such as immunodiffusion or an immunoassay. 2. The absorbed protein peak containing PSA is then lyophilised, redissolved in Tris-HCl buffer and chromatographed in a Superdex-75 or Sephadex-75 column. The absorbed proteins elute out as multiple peaks and PSA is eluted as a sharp peak.At each stage, PSA has to be identified by an independent method such as immunodiffusion or an immunoassay. 3. Step 2 is repeated for better purity. 4. The PSA peak is lyophilised, dissolved in Tris-HCl buffer without NaCl and further purified on an ion exchange column (either anion or cation exchange columns such as DEAE Sephadex or CM-Sephadex or Mono Q). Gradient elution using Tris-HCl buffer without NaCl and Tris-HCl buffer with 0.25 M NaCl resulted in a sharp pure PSA peak (homogenous, sharp single band on SDS-PAGE). This procedure is based on that reported by Wang et al., Oncology, 39,1,1982

  17. Human immunodeficiency virus type-1 episomal cDNA in semen

    Directory of Open Access Journals (Sweden)

    Mayer Kenneth H

    2005-10-01

    Full Text Available Abstract Background Episomal 2-long terminal repeat (LTR HIV-1 cDNA, a by-product of HIV-1 infection, is used in clinical trials as a marker for ongoing viral replication. It would be useful to employ 2-LTR cDNA to monitor cryptic HIV-1 infection in the genital tract of men on antiretroviral therapy (ART to predict the evolution of sexually transmissible drug-resistant HIV-1, but studies thus far have failed to detect this marker in semen. The objectives of this study were: 1 to use a technique that maximizes DNA recovery from HIV-1 infected white blood cells in semen to determine if episomal 2-LTR cDNA is detectable in semen of ART-naïve men with other evidence of genital tract HIV-1 infection, and 2 to compare levels of HIV-1 2-LTR cDNA, RNA, and proviral DNA in semen from HIV-1+ men on ART. Results Using a somatic cell DNA extraction technique, 2-LTR cDNA was detected by PCR/ELISA in 4 out of 8 semen samples from ART-naïve men selected for other signs of seminal HIV-1 infection (positive controls. Southern blot and DNA sequencing confirmed that the amplified sequences were HIV-1 2-LTR cDNA; copy numbers ranged from 55 to 504 copies/sample. Two semen samples from a cohort of 22 HIV-1-infected men on dual nucleoside therapy, one with and one without detectable seminal HIV-1 RNA, were 2-LTR cDNA positive (336 and 8,560 copies/sample. Following addition of indinavir to the therapy regimen, no semen samples from 21 men with controlled peripheral and seminal viral loads were 2-LTR cDNA positive at 1 and 6 month time points, despite the persistence of HIV-1 proviral DNA+ semen cells and seminal cytomegalovirus (CMV shedding in some cases. However, one individual who failed indinavir therapy and later developed distinct protease inhibitor (PI drug resistance mutations in semen, maintained elevated levels of HIV-1 RNA and 2-LTR cDNA in semen. Conclusion 2-LTR HIV-1 cDNA is detectable in semen of HIV-1-infected men. Two men on ART had 2-LTR HIV-1 cDNA in semen, suggesting that this marker may prove to be useful to monitor HIV-1 infection in the genital tract of men on ART to predict the evolution of drug resistance mutations in semen.

  18. A Study of a Method to Assess the Purity of Sorted Bovine Semen Using Rapid Single-Sperm Sexing PCR

    Directory of Open Access Journals (Sweden)

    Weihua Du

    2011-01-01

    Full Text Available Sort reanalysis using flow cytometry is the most common method for determining the purity of X or Y enriched semen. The high cost of this technique (including the required expensive, proprietary machine limits efforts to improve the technique and to promote develop applications for the sorted semen. In this study, the sperm sex (the presence of the X or Y chromosome was identified by both rapid PCR and flow cytometry reanalysis. The rapid PCR results showed that the percentages of X and Y sperm were 48 and 52% in unsorted semen, 92 and 8% in X-enriched semen and 17 and 83% in Y-enriched semen, respectively. Reanalysis of the DNA content of the sorted samples revealed that the X and Y sperm frequencies were 92 and 8% in X-enriched semen and 15 and 85% in Y-enriched semen, respectively. The sex ratio of unsorted semen analyzed by PCR did not significantly deviate from the expected ratio of 1:1 and there was no significant difference between the sex ratios of sorted semen samples determined by PCR and flow cytometry reanalysis. These results indicate that we have established an effective, reliable and rapid PCR method to verify the purity of sorted semen. This method should contribute greatly to the improvement of sperm sorting techniques and the development of applications for sorted semen.

  19. Effects of distilled Phaseoli Semen rubra Herbal-Acupuncture on lipid composition, liver function, antioxidant capacity and molecular biological aspects in obese rats induced high fat diet

    OpenAIRE

    Ji, Jun Hwan; Lee Jun Mu

    2005-01-01

    Effects of Phaseoli Semen rubra Herbal-acupuncture at zusanli(ST-36), Quchi(LI-11) and Sanyinjiao(Sp-6) on lipid composition, liver function, oxidative capacity and molecular biological aspects were investigate in high fat diet induced obese rats. Forty male Sprague-Dawley rats weighing about 400g were divided into 4 groups according to body weight and raised four weeks with control, zusanli(ST-36), Quchi(LI-11) and Sanyinjiao(Sp-6) Herbal-acupuncture groups. 1. Plasma total cholesterol an...

  20. Impact of chronic viral diseases on semen parameters.

    Science.gov (United States)

    Lorusso, F; Palmisano, M; Chironna, M; Vacca, M; Masciandaro, P; Bassi, E; Selvaggi Luigi, L; Depalo, R

    2010-04-01

    The aim of this study was to assess the effect of human immunodeficiency virus (HIV), hepatitis C (HCV) and B (HBV) virus infection on semen parameters. Semen samples were obtained from 27 HCV, 34 HIV, 30 HBV and 41 HCV-HIV-seropositive patients and compared with those of a control population of healthy seronegative subjects. Tests for detection of HIV, HCV and HBV were performed on seminal samples. The sperm concentration was significantly decreased in HCV- and HBV-seropositive males compared to that of controls (P sperm motility (a + b) was significantly decreased in HCV- and HBV-seropositive (P sperm viability was significantly lower in HCV- and HBV-seropositive men than in controls (P sperm concentration after sperm wash was significantly higher in controls than in HCV-, HIV-, HBV- and HIV-HCV-seropositive men (P sperm quality compared with that of controls. The reason for the better sperm quality in our series of HIV- and HCV-HIV-infected men is still under debate. Further investigations in a larger case series are warranted. PMID:20384803

  1. Differential protein profile in sexed bovine semen: shotgun proteomics investigation.

    Science.gov (United States)

    De Canio, Michele; Soggiu, Alessio; Piras, Cristian; Bonizzi, Luigi; Galli, Andrea; Urbani, Andrea; Roncada, Paola

    2014-06-01

    The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein repertoire for the development of improved methods to differentiate X and Y sperm cells maintaining high vitality. In order to address this issue, we performed a comparative shotgun proteomic investigation by nUPLC-MS/MS to characterize sexed bovine semen. The protein profiles of these two types of sperm cells have shown differential expression of proteins that may be directly associated with the main components of cytoskeletal structures of flagellum, as the axoneme, outer dense fibers and fibrous sheath, as well as glycolytic enzymes and calmodulin, involved in the energetic metabolism regulation. Overall these results may provide a base to a better comprehension of the biological features of sperm cells and may be useful to the development of alternative methods of separation. PMID:24226273

  2. Semen cryopreservation in the Indian rhinoceros (Rhinoceros unicornis).

    Science.gov (United States)

    Stoops, M A; Atkinson, M W; Blumer, E S; Campbell, M K; Roth, T L

    2010-05-01

    The objective was to identify an extender and cryoprotectant combination for Indian rhinoceros (Rhinoceros unicornis) sperm that yielded high post-thaw sperm quality. Male Indian rhinoceroses (n=6; 7.5-34 yr old) were anesthetized and subjected to a regimented electroejaculation procedure (75-100 mAmps; 4-10 volts; 7-150 stimuli; total of 10 electroejaculation procedures). High quality semen fractions from each ejaculate were divided into four aliquots and a 2 x 2 factorial design used to compare the effect of two sperm extenders (standard equine [EQ] and skim milk-egg-yolk-sugar [SMEY]), and two cryoprotectants (glycerol and dimethylsulfoxide [DMSO]). Cyropreserved samples were thawed and assessed for motility, viability and acrosome integrity over time. Electroejaculate fractions processed for cryopreservation had high sperm concentration (516 x 10(6)/mL) and motility (79%). Post-thaw sperm characteristics were higher (P<0.05) when semen was cryopreserved in EQ versus SMEY. Post-thaw motility of sperm cyropreserved in EQ averaged 50-55% compared to 22-37% in SMEY, with no significant differences in sperm characteristics of samples cyropreserved in glycerol and DMSO. In conclusion, sperm collected from Indian rhinoceroses via electroejaculation were cryopreserved using EQ extender with either glycerol or DMSO; post-thaw quality was adequate for use in assisted reproductive procedures. PMID:20172598

  3. Semen quality and heavy metal and pesticides toxicity

    Directory of Open Access Journals (Sweden)

    Lidia Mnguez-Alarcn

    2014-06-01

    Full Text Available Male reproductive function has deteriorated significantly in the past 50 years and this change could be related to an exposure to occupational and environmental pollutants and toxicants. The purpose of this paper is to summarize the negative impact of human exposure to heavy metals and pesticides on the male reproductive function. Most pesticides and heavy metals are considered reproductive toxicants and may adversely harm the male reproductive system due to their disrupting effect on the hypothalamus- pituitary gland-gonads axis or by directly affecting spermatogenesis, resulting in impaired semen quality. The negative effects of these compounds have been linked to the main sperm parameters (concentration, normal morphology and motility, semen volume and total sperm count and DNA sperm damage, as well as to changes in serum reproductive hormone levels. Some of these substances have already been banned, whereas others are still on the market. Stricter laws are needed to completely prevent exposure to these toxicants given their proven deleterious effect on male reproductive health.

  4. Laser researches on livestock semen and oocytes: A brief review

    Directory of Open Access Journals (Sweden)

    Z. Abdel-Salam

    2015-05-01

    Full Text Available This article presents a brief review of the past and present literature pertinent to laser effects on sperm motility parameters, improvement of oocyte maturation and characterization of semen in livestock. The aim was, on one hand, to make the readers aware of such knowledge and on the other hand to trigger the interest of the animal reproduction scientific community in attempting some laser techniques that have not yet been fully exploited in the field of artificial insemination. With respect to the conventional methods, laser is a more sensitive and less costly technology that can be used for improving artificial insemination and embryo production system. Since 1980s, laser treatment came on the biological samples scene; its applications have continuously been developed thereafter. Exploitation of laser light by various researchers for improving the reproductive efficiency of sperm cells and the maturation rate in different livestock is demonstrated herein. Laser irradiation, in principal, can increase the production of adenosine triphosphate (ATP and consequently increases the energy provided to the cell. Since sperm motility and oocyte maturation depend on the energy consumption, an increase in the energy supply to the cells will be of great importance. In addition, the authors also discuss the use of laser spectrochemical analytical techniques, such as laser induced breakdown spectroscopy (LIBS and laser induced fluorescence (LIF, in characterization of semen samples.

  5. Human semen hyperviscosity: prevalence, pathogenesis and therapeutic aspects.

    Science.gov (United States)

    Elia, Jlenia; Delfino, Michele; Imbrogno, Norina; Capogreco, Francesca; Lucarelli, Marco; Rossi, Tiziana; Mazzilli, Fernando

    2009-09-01

    The aims of this study were (a) to determine the prevalence of subjects with semen hyperviscosity (SHV) in a large population of male partners of subfertile couples; (b) to identify any correlation between SHV and infections or inflammation of the genital tract; (c) to assess the effects of therapeutic approaches for treating SHV; and (d) to assess sperm kinetic parameters after successful treatment of SHV. A retrospective study of 1 833 male partners of subfertile couples was conducted. Next, clinical, seminal, bacteriological and ultrasound studies involving 52 subjects suffering from SHV were performed, and the SHV was classified as being mild (length of thread > 2 cm and 4 cm and 6 cm). The prevalence of SHV was observed in 26.2% (480) of the subjects, with 13.2% suffering from mild, 6.6% from moderate and 6.4% from severe SHV. Treatment was completely successful in only 27 subjects (52.0%), primarily in those who had mild basal SHV with a positive semen culture. In these subjects, progressive motility percentage, straight line velocity and linearity were significantly higher than pre-treatment levels. SHV is often found in subjects with subfertility. Pathogenesis was strictly related to infective/inflammatory factors in only 48.0% of cases; therefore, it is possible that biochemical, enzymatic or genetic factors have a role in this condition. PMID:19701219

  6. Evaluation of Semen Fertility of Bulls by Non-return Rate at 60 Days of Cows under Artificial Insemination Programme in Bangladesh

    Directory of Open Access Journals (Sweden)

    M.J.U. Sarder

    2006-01-01

    Full Text Available The present study were to evaluate the effect of individual bull, semen types, quality of bull semen, sources of semen on Non-return Rate (NRR at 60 days of cows under field condition. A total 75550 cows were inseminated with 71 bull semens from Central Cattle Breeding Station and Dairy Farm (CCBSDF, Savar, Dhaka, Rajshahi Dairy and Cattle Improvement Farm (RDCIF, Rajabarihat and District Artificial Insemination Centre (DAIC, Rajshahi under 40 Artificial Insemination (AI sub-centres/points of District AI centre, Rajshahi. The overall NRR was obtained 78.54% with chilled and frozen semen produced from three AI centres/stations. Analysis of variance showed that individual bull semen had significant (p<0.05 effect on NRR at 60 days after first insemination. Semen types, quality of bull semen and sources of semen had significant (p<0.001 effect on NRR at 60 days of cows. The significant (p<0.001 highest NRR (82.32% was with chilled semen and lowest was with frozen semen (76.39%. The significant (p<0.001 maximum NRR (83.12% was for the best quality bull semen and minimum (70.13% for the poor quality bull semen. Significant (p<0.001 higher NRR (82.32% was in semen from DAIC, Rajshahi and lower (73.01% in semen from RDCIF, Rajabarihat. Results suggested that the NRR of cows at 60 days after first insemination under field condition may be a good practice to discard poor fertility semen among the individual bull semen, semen types (chilled and frozen, quality of bull semen (poor, good and best and sources of semen (CCBSDF, Savar, RDCIF, Rajabarihat and DAIC, Rajshahi for artificial insemination programme in Bangladesh.

  7. Effect of contaminated preprocessed semen on fertilization rate and embryo quality in assisted reproductive techniques.

    Science.gov (United States)

    Krissi, H; Orvieto, R; Ashkenazi, J; Gilboa, Y; Shalev, J; Moscovitch, I; Bar-Hava, I

    2004-02-01

    We aimed to identify the sources and prevalence of semen contamination from mastrubation and determine the effect of bacterospermia on fertilization rate and embryo quality in standard in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). This was a prospective controlled study, in an IVF unit of a university teaching hospital, of 93 consecutive couples undergoing IVF-embryo transfer cycles. We evaluated handwashing; semen collection and processing; and assisted reproductive technology using semen provided by masturbation. The main outcome measures were presence and type of micro-organisms in the semen samples and embryo culture medium; the effect of hand washing on rate of contamination; and the effect of semen contamination on fertilization rate and embryo quality. The first consecutive 52 men of the 93 couples were not instructed to wash their hands before masturbation, and the remainder were so instructed. Forty-nine semen cultures (94.2%) in the first group were contaminated compared to only 16 (39%) in the second (p < 0.016); 27 of the 65 positive cultures (41.5%) were contaminated by more than one organism. The most common contaminators were bacteria usually found on the skin. All but four embryo medium cultures were negative. There was no significant difference in fertilization rate and embryo quality by culture findings in either the IVF or the ICSI procedures. We found that a high percentage of manually obtained semen for standard IVF or ICSI procedures was contaminated, but this had no effect on fertilization rate and embryo quality. PMID:15195496

  8. Do malignant diseases affect semen quality? Sperm parameters of men with cancers.

    Science.gov (United States)

    Caponecchia, L; Cimino, G; Sacchetto, R; Fiori, C; Sebastianelli, A; Salacone, P; Marcucci, I; Tomassini, S; Rago, R

    2016-04-01

    The advent of modern treatments together with the improvement of the surgical techniques has significantly increased 5-year survival rates of young patients with cancer. Although the deleterious effects of chemotherapy and radiation are well documented, controversies exist about the effect of cancer itself on semen parameters before treatment. We collected data on 236 patients representative of different types of cancers reoffered at our institution for sperm cryopreservation with the aim to correlate the pre-freeze semen parameters with type of cancer, disease stage and with semen quality of 102 fertile and healthy men. The median baseline semen parameters of all our patients with cancer are placed above the 5th percentile of the World Health Organization reference value, but the type of cancer may impact the sperm parameters. In testicular tumours and in Hodgkin lymphoma, we show a semen concentration statistically lower than in the fertile population, while in patients with other cancers, there is no difference with the healthy men. We found no correlation between semen quality and disease stage. Eighty-six per cent of our patients do not have children at the time of semen cryopreservation, and the only established clinical option for preserving fertility of these men is cryopreservation of spermatozoa. PMID:26173956

  9. Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen.

    Science.gov (United States)

    Nabi, Mohammad Mahdi; Kohram, Hamid; Zhandi, Mahdi; Mehrabani-Yeganeh, Hassan; Sharideh, Hossein; Zare-Shahaneh, Ahmad; Esmaili, Vahid

    2016-02-01

    Two experiments were conducted to evaluate the new rooster semen freezing extender which is containing a low level of glycerol and soybean lecithin as an alternative protective agent in the extender. The aim of the first experiment was to evaluate a new extender for freeze-thawing rooster semen known as "Nabi" extender compared to Beltsville. Second experiment was also performed to determine whether the Nabi extender has negative reactions on fertilization after artificial insemination (AI) or no. In the first experiment, post-thaw motion parameters, mitochondrial function and sperm apoptosis were analyzed using Sperm Class Analyzer (SCA), rhodamine-123 and Annexin-V, respectively for frozen-thawed semen in Nabi and Beltsville extender. Results showed that total motility, progressive motility, velocity parameters (VCL, VSL, VAP, LIN and STR) and live spermatozoa with active mitochondria were significantly higher in Nabi compare to Beltsville extender (Pinseminated with thawed semen using the new freezing diluents or fresh semen for determination of fertility rate. Fertility rate with thawed semen (with Nabi extender) was lower compared to fresh semen (by approximately 8% points). It can be concluded that Nabi extender would improve post-thawed rooster sperm invitro quality compared to Beltsville extender. The fertility rates of insemination in hens with freeze-thaw sperm were comparable with fresh sperm. PMID:26632488

  10. Poor acceptance rate for semen donors to a private cryobank in Nigeria.

    Science.gov (United States)

    Akinrinola, O A; Melie, N A; Ajayi, R A

    2003-04-01

    A retrospective analysis of sixty one prospective donors who visited the cryobank in person from August 1999 to December 2001 was done. The study evaluated the acceptance rate of prospective semen donors and semen characteristics in order to assess the fertility potential of the supposedly normal population of young men. The outcome of the various screening processes--history taking, infection screening, blood grouping and Hb genotype assessment, semen analyses (pre-freeze and post-thaw), and post-quarantine (six months after production of last sample) infection--was analysed. Out of the 61 enquiries, 9 (14.8%) donors were recruited, 7 (77.8%) of whom were less than 25 years old. Fourteen (22.9%) donors had sperm concentrations greater than 60' 10(6) ml, 31 (50.8%) had motility greater than 60% and 17 (27.8%) had normal forms greater than 30%. Six (9.8%) of the donors were unable to masturbate and thus were disqualified, while only one donor (6.1%) failed to show up for a re-test after the completion of the quarantine period. Twenty one (38.2%) donors had low semen values based on the WHO reference value. We conclude that a high proportion of prospective semen donors may have suboptimal semen parameters, and that using strict criteria, poor acceptance rates for donors is observed in a semen donation programme. PMID:12816309

  11. Characterization of lactate dehydrogenase enzyme in seminal plasma of Japanese quail (Coturnix coturnix japonica).

    Science.gov (United States)

    Singh, R P; Sastry, K V H; Pandey, N K; Shit, N; Agrawal, R; Singh, K B; Mohan, Jag; Saxena, V K; Moudgal, R P

    2011-02-01

    Lactate dehydrogenase enzyme present in quail seminal plasma has been characterized. Polyacrylamide gel electrophoresis and subsequently with LDH specific staining of seminal plasma revealed a single isozyme in quail semen. Studies on substrate inhibition, pH for optimum activity and inhibitor (urea) indicated the isozyme present in the quail semen has catalytic properties like LDH-1 viz. H-type. Furthermore, unlike other mammalian species, electrophoretic and kinetic investigations did not support the existence of semen specific LDH-X isozyme in quail semen. The effect of exogenous lactate and pyruvate on sperm metabolic activity was also studied. The addition of 1 mM lactate or pyruvate to quail semen increased sperm metabolic activity. Our results suggested that both pyruvate and lactate could be used by quail spermatozoa to maintain their basic functions. Since the H-type isozyme is important for conversion of lactate to pyruvate under anaerobic conditions it was postulated that exogenous lactate being converted into pyruvate via LDH present in semen may be used by sperm mitochondria to generate ATP. During conversion of lactate to pyruvate NADH is being generated that may be useful for maintaining sperm mitochondrial membrane potential. PMID:21074838

  12. Effect of green buffer storage on the fertility of fresh camel semen after artificial insemination.

    Science.gov (United States)

    Morton, K M; Billah, M; Skidmore, J A

    2011-06-01

    Artificial insemination (AI) is one of the most widely used reproductive technologies, and there is considerably interest in commercializing this technology in camels. Storage of semen extender frozen (at -20 °C) is of considerable interest to scientists working with camels, as transportation of diluents at refrigeration temperature is not always possible given the hot, arid and remote conditions that dromedary camels exist in. Therefore, this study was conducted to compare the fertility of fresh camel semen, after dilution in fresh or frozen-thawed green buffer (GB), after AI into single and multiple ovulating female camels. No differences were observed in any sperm characteristics (motility, membrane integrity, acrosome integrity or morphology) when semen was diluted in fresh or frozen-thawed GB (p>0.05). Sperm motility was increased by dilution (fresh: 70.7 ± 4.9% and frozen: 68.8 ± 3.1%) compared with the motility of sperm in neat semen (35 ± 2.85%; pcamels inseminated with semen diluted in fresh (72.7%) compared with frozen-thawed GB (27.3%), and fertilization rates were also higher (pcamels inseminated with semen diluted in fresh (83.3%) compared with frozen-thawed GB (11.1%). These results clearly demonstrate the detrimental effect of freezing and thawing semen diluent on the fertility of fresh camel semen. However, further studies are required to elucidate the mechanism responsible for this reduction in fertility. Moreover, these results demonstrate that the fertility of fresh camel semen diluted in fresh GB is high enough to be considered commercially viable. PMID:21545653

  13. The reference values for semen parameters of 1213 fertile men in Guangdong Province in China

    Directory of Open Access Journals (Sweden)

    Yun-Ge Tang

    2015-04-01

    Full Text Available Semen samples were collected from 1213 fertile men whose partners had a time-to-pregnancy (TTP ?12 months in Guangdong Province in Southern China, and semen parameters including semen volume, sperm concentration, total counts, motility, and morphology were evaluated according to the World Health Organization (WHO 2010 guideline. All semen parameters analyzed were normal in ~62.2% of the total samples, whereas ~37.8% showed at least one of the semen parameters below normal threshold values. The fifth centiles (with 95% confidence intervals were 1.3 (1.2-1.5 ml for semen volume, 20 10 6 (1810 6 -2010 6 ml?1 for sperm concentration, 40 10 6 (3810 6 -4410 6 per ejaculate for total sperm counts, 48% (47%-53% for vitality, 39% (36%-43% for total motility, 25% (23%-27% for sperm progressive motility, 5.0% (4%-5% for normal morphology. The pH values ranged from 7.2 to 8.0 with the mean standard deviation at 7.32 0.17. No effects of age and body mass index were found on semen parameters. Occupation, smoking and alcohol abuse, varicocele appeared to decrease semen quality. Sperm concentration, but not sperm morphology, is positively correlated with TTP, whereas vitality is negatively correlated with TTP. Our study provides the latest reference values for the semen parameters of Chinese fertile men in Guangdong Province, which are close to those described in the new WHO guidelines (5 th Edition.

  14. Diagnosis and effects of urine contamination in cooled-extended stallion semen.

    Science.gov (United States)

    Ellerbrock, R; Canisso, I; Feijo, L; Lima, F; Shipley, C; Kline, K

    2016-04-15

    Urospermia is known to affect semen quality in many mammals, including stallions. Determinations of semen pH and creatinine and urea concentrations have been used to diagnose urine contamination in raw stallion semen. Unfortunately, practitioners suspecting urine contamination in cooled-shipped samples have no proven means to confirm the presence of urine. Therefore, the objectives of this study were (1) to assess the effects of urine contamination on sperm motility of extended fresh and cooled-stored stallion semen, (2) to evaluate the usefulness of semen color, odor, pH, and creatinine and urea concentrations for urospermia diagnosis, and (3) to evaluate the accuracy of a commercial blood urea nitrogen test strip in diagnosing urine contamination in extended-cooled stallion semen. Thirty-seven ejaculates were obtained from 11 stallions with no history of urospermia before division into 5 mL aliquots, and contamination with stallion urine. Each resulting sample was assessed for sperm motility, color, odor, pH, creatinine, and urea nitrogen concentration using both a semiquantitative test strip (Azostix), and a quantitative automated analyzer before and after cooling for 24 hour. Sperm motility parameters, pH, and creatinine and urea concentrations were analyzed using mixed models. Urine contamination decreased total and progressive motility in all samples before and after cooling (P cooled-stored stallion semen. Sperm motility parameters (in raw and cooled semen) are significantly reduced by the presence of urine in a concentration dependent. The results of the present study indicated that determination of urea and creatinine concentrations can be used to diagnose urospermia and that Azostix can be used as a point care method for diagnosing urine contamination in extended cooled stallion semen. PMID:26764152

  15. Kualitas Semen Beku Kuda pada Pengencer Susu Skim dengan Konsentrasi Gliserol yang Berbeda

    Directory of Open Access Journals (Sweden)

    Azizah -

    2009-06-01

    Full Text Available Cryoprotectant is one of determining factors for the success of stallion semen cryopreservation. Formost mammalian sperm cryopreservation,glycerol has been widely used as the cryoprotectant. Glycerol inhigh concentration is toxic to the sperm but in low concentration it has protective effect on the semen. Theobjectives of this experiment were to find appropiate glycerol concentration by using skim milk extender inorder to maintain motility and viability of spermatozoa of stallion frozen semen. Semen was collectedfrom three sexually mature stallions by using artificial vagina. The semen characteristics and qualitywere examined both macro and microscopically. Prior to extension, semen was centrifugated at 3000 rpmfor 15 minutes. The pellet was re-extended with skim milk extender with glycerol 5% (G5 7.5% (G7.5, and10% (G10. The extended semen as then packed in mini straw (0.3ml, equilibrated at 5 oC for 2 hours,frozen in the liquid N2 vapor for15 minutes and then stored in liquid N2 container until futher evaluation.The frozen semen, was thawed at 37 oC for 30 seconds. The percentages of sperm motility and viabilitywere examihed. The result of this research showed that the sperm motility and viability in G5 was (23.8 %;35.6 % significantly hihger (P<0.05 as compare a with G7.5 (18.1%; 28.6% and G10. (11.8%; 23.1%. Therecovery Rate in G5 (35.4% was significant high er (P<0.05 than G7.5 (26.9% and G10 (17.6%. In conclusion,glicerol of 5% is recomended for cryopreservation of stallion semen extended with skim milk.

  16. Improvement of the Shami goat semen quality by adding bovine serum albumin

    Directory of Open Access Journals (Sweden)

    O.I. Azawi

    2014-06-01

    Full Text Available The present study was aimed to improve the quality of Shami goat semen diluted with Tris diluent by adding bovine serum albumin. In the current study, six male goats were used. Semen was collected using artificial vagina of one ejaculate per week of every male included in this study. This study was performed during the breeding season from 1 \\ 10 \\ 2012 to 1 \\ 12 \\ 2012. In this study, two semen diluents were use first; Tris- fructose- egg yolk 2.5% and second Tris - fructose - 2.5% egg yolk with 1% of bovine serum albumin. Diluted semen samples were cooled gradually and stored at 5 ° C. Cooled diluted semen samples were examined every 24 h of storage to 144 h. These tests includes the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility using a computer-aided sperm analysis. These results showed that the addition of bovine serum albumin with egg yolk to semen of male goats led to improved qualities of semen significantly (P<0.05 including the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility. It could be concluded from the results of the current study, the possibility of storing goat semen for more than six days with alive sperm of more than 50% and the percentage of the progressive motility of more than 40% when adding bovine albumin serum to dilute goat semen at 1% level and this result has not reached by any previous study.

  17. Effect of the Type of Straw on the Spermatic Quality in the Freezing of Boar Semen

    Directory of Open Access Journals (Sweden)

    C.A. C?rdova-Jim?nez

    2006-01-01

    Full Text Available With the freezing boar semen, could have better options for the optimization of the reproductive handling in the swinish species as well as an alternative for the development of this cattle activity; using technologies like the implementation of banks of frozen of races with characteristic zootechnic of economic importance that guarantee the readiness of germinal material in the moment that is required, to have germinal material of males proven genetically, still when the animal no longer exists, to overcome certain intentional restrictions of transport of alive animals, for the problem of transmission of illnesses and, to overcome the restrictive of time of viability of the diluted fresh semen. In this work was examined the effect of the freezing boar semen in straws plastic of 0.5 and 5 mL on the Motility and the Acrosome Integrity (NAR. For it, 9 were used ejaculated of different animals, the experiment was carried out comparing fresh semen with thawing semen coming from straws of 0.5 and 5 mL. The results of percentages of motility and NAR for fresh and thawing semen, were of 86.19, 47.14 and 47.14, for straws of 0.5 mL and 75.62, 48.19 and 46.81, for straws of 5 mL. When carrying out the analysis of the variance and the test of multiple comparisons it was found that the freezing of the semen in both straws types, the percentages of motility and NAR reduce, with regard to the fresh semen; however, the macrotubes or straws of 5 mL, represent a good option in the artificial insemination using boar semen frozen-thawing.

  18. Semen evaluation in four autochthonous wild raptor species using computer-aided sperm analyzer.

    Science.gov (United States)

    Dogliero, Andrea; Rota, Ada; Lofiego, Renato; Mauthe von Degerfeld, Mitzy; Quaranta, Giuseppe

    2016-04-01

    At least 10 percent of the approximately 300 species of the order Falconiformes are listed as being globally threatened. The present work describes the seminal characteristics of three diurnal and one nocturnal raptor species. Semen was collected from clinically healthy Accipiter nisus (n = 1), Falco subbuteo (n = 6), and Falco tinnunculus (n = 5) adult males that were housed at the 'Centro Animali Non Convenzionali' of the Department of Veterinary Sciences of the University of Turin. The semen was collected after a period of recovery and before their release as well as from seven Bubo bubo males bred in captivity as part of a raptor conservation project. All the potential semen donors were trained in semen collection during the breeding season via a ritualized procedure. Ejaculation was achieved using a massaging technique. Each sample was evaluated for volume, degree of contamination, and spermatozoa concentration. The semen motility and kinetic parameters were assessed on diluted semen (modified tyroides albumin lactate pyruvate, pH 7.5, temperature 37.5 °C) using a computer-aided sperm analyzer. Semen collection was successful in all the diurnal species and in five B bubo individuals. The sperm motility and sperm kinetic parameters were very variable both among and within species. In contrast with previous studies that involved raptors bred in captivity and imprinted on humans, we worked with wild birds and attempted to overcome the problem of poor semen quality, which is strongly influenced by stress, by adopting a ritualized procedure that has never been reported for semen collection purposes. PMID:26725000

  19. An Outbreak of Porcine Reproductive and Respiratory Syndrome Virus in Switzerland Following Import of Boar Semen.

    Science.gov (United States)

    Nathues, C; Perler, L; Bruhn, S; Suter, D; Eichhorn, L; Hofmann, M; Nathues, H; Baechlein, C; Ritzmann, M; Palzer, A; Grossmann, K; Schüpbach-Regula, G; Thür, B

    2016-04-01

    An outbreak of porcine reproductive and respiratory syndrome virus (PRRSV) occurred in November 2012 in Switzerland (CH), traditionally PRRSV-free. It was detected after a German boar stud informed a semen importer about the detection of PRRSV during routine monitoring. Tracing of semen deliveries revealed 26 Swiss sow herds that had used semen from this stud after its last negative routine monitoring and 62 further contact herds. All herds were put under movement restrictions and examined serologically and virologically. As a first measure, 59 sows from five herds that had previously been inseminated with suspicious semen were slaughtered and tested immediately. Investigations in the stud resulted in 8 positive boars with recent semen deliveries to CH (Seven with antibodies and virus, one with antibodies only). In one boar out of six tested, virus was detected in semen. Of the 59 slaughtered sows, five from three herds were virus-positive. In one herd, the virus had spread, and all pigs were slaughtered or non-marketable animals euthanized. In the remaining herds, no further infections were detected. After confirmatory testings in all herds 3 weeks after the first examination gave negative results, restrictions were lifted in January 2013, and Switzerland regained its PRRSV-free status. The events demonstrate that import of semen from non-PRRS-free countries - even from negative studs - poses a risk, because monitoring protocols in boar studs are often insufficient to timely detect an infection, and infections of sows/herds occur even with low numbers of semen doses. The outbreak was eradicated successfully mainly due to the high disease awareness of the importer and because immediate actions were taken before clinical or laboratory diagnosis of a single case in the country was made. To minimize the risk of an introduction of PRRSV in the future, stricter import guidelines for boar semen have been implemented. PMID:25209832

  20. Cryopreserving turkey semen in straws and nitrogen vapour using DMSO or DMA: effects of cryoprotectant concentration, freezing rate and thawing rate on post-thaw semen quality.

    Science.gov (United States)

    Iaffaldano, N; Di Iorio, M; Miranda, M; Zaniboni, L; Manchisi, A; Cerolini, S

    2016-04-01

    1. This study was designed to identify a suitable protocol for freezing turkey semen in straws exposed to nitrogen vapour by examining the effects of dimethylacetamide (DMA) or dimethylsulfoxide (DMSO) as cryoprotectant (CPA), CPA concentration, freezing rate and thawing rate on in vitro post-thaw semen quality. 2. Pooled semen samples were diluted 1:1 (v:v) with a freezing extender composed of Tselutin diluent containing DMA or DMSO to give final concentrations of 8% or 18% DMA and 4% or 10% DMSO. The semen was packaged in 0.25 ml plastic straws and frozen at different heights above the liquid nitrogen (LN2) surface (1, 5 and 10 cm) for 10 min. Semen samples were thawed at 4°C for 5 min or at 50°C for 10 s. After thawing, sperm motility, viability and osmotic tolerance were determined. 3. Cryosurvival of turkey sperm was affected by DMSO concentration. Freezing rate affected the motility of sperm cryopreserved using both CPAs, while thawing rates showed an effect on the motility of sperm cryopreserved using DMA and on the viability of sperm cryopreserved using DMSO. Significant interactions between freezing rate × thawing rate on sperm viability in the DMA protocol were found. 4. The most effective freezing protocol was the use of 18% DMA or 10% DMSO with freezing 10 cm above the LN2 surface and a thawing temperature of 50°C. An efficient protocol for turkey semen would improve prospects for sperm cryobanks and the commercial use of frozen turkey semen. PMID:26872498

  1. Dose-dependent effects of tamsulosin on the ejaculation and semen quality in bucks

    OpenAIRE

    KIMSAKULVECH, SAKDICHOD; SUTTIYOTIN, PEERASAK; Pinyopummin, Anuchai

    2015-01-01

    The objective of this study was to investigate the effect of tamsulosin (TAM) dosages on ejaculation and semen quality. Two sets of Latin square experiment design were applied to 6 bucks receiving normal saline (CON), TAM 134.8 nM/kg (LTAM), or 269.7 (HTAM) nM/kg at 1-week intervals. Semen collection, libido, and ejaculatory scoring were undertaken at 3, 6, 9, 12, and 24 h after injection. Heart rate and body temperature were measured before administration and at 30 min, and before semen coll...

  2. Effect of extenders, postdilution intervals, and seasons on semen quality in dairy goats

    OpenAIRE

    QURESHI, Muhammad Subhan; Khan, Daulat; MUSHTAQ, Anila; AFRIDI, Shoaib Sultan

    2013-01-01

    The breeding of female dairy goats with good quality sires has been a problem in rural areas and artificial insemination (AI) provides an answer to this issue. To develop an AI model, a total of 9 bucks were purchased from a local market. Semen was collected in an artificial vagina, the libido was noted, and the semen was evaluated for its physical characteristics. The libido, semen volume, mass motility, individual motility, and sperm concentration were 2.94 0.25 (scale of 1-3), 0.79 0.5...

  3. Evaluation of semen quality in 1808 university students, from Wuhan, Central China

    OpenAIRE

    Meng Rao; Tian-Qing Meng; Si-Heng Hu; Huang-Tao Guan; Qin-Yu Wei; Wei Xia; Chang-Hong Zhu; Cheng-Liang Xiong

    2014-01-01

    The aim of this study was to evaluate the semen quality of university students in Wuhan, the largest city in the world in terms of the number of university students. All student sperm donors recorded in the Hubei Province Human Sperm Bank from 1 March 2010 to 31 December 2013 were screened. At last, a total of 3616 semen samples from 1808 university student sperm donors were eligible and retrospectively analyzed. Each donor's semen parameters were averaged over two samples and compared with t...

  4. EFFECT OF LOW DENSITY LIPOPROTEINS IN EXTENDER ON FREEZABILITY AND FERTILITY OF EGYPTIAN BUFFALO BULL SEMEN

    OpenAIRE

    El-Seify; I. M.; Abd El-Razek; M.A.R.; Ibrahim ,; I S; El-Shamaa; M. E.; El-Sharawy; E.M.

    2012-01-01

    Semen from three Egyptian buffalo bulls was collected once weekly and ejaculates with more 75% progressive motility and more 85 % normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bulls effect. Seven extenders were used: Tris 20 % egg yolk extender with 7 ml glycerol as a control (T1), and substitution of whole egg yolk with 4, 6, 8, 10, 12 and 15 % low density lipoprotein (LDL), T2 – T6, respectively. Semen wa...

  5. Detection of boar sperm plasma membrane protein using Rhodamine 640; implications for cryobiology and physiology

    Science.gov (United States)

    Rhodamine 640 (R640) was used to detect changes in boar sperm plasma membrane protein (PMP) during cryopreservation; a poorly understood phenomenon. The protocol was adapted for boar sperm so that semen samples (n = 17) could be analyzed for PMP (R640 positive) and plasma membrane integrity (PMI; Y...

  6. Liquid storage of boar semen: Current and future perspectives on the use of cationic antimicrobial peptides to replace antibiotics in semen extenders.

    Science.gov (United States)

    Schulze, M; Dathe, M; Waberski, D; Mller, K

    2016-01-01

    Antibiotics are of great importance in boar semen extenders to ensure long shelf life of spermatozoa and to reduce transmission of pathogens into the female tract. However, the use of antibiotics carries a risk of developing resistant bacterial strains in artificial insemination laboratories and their spread via artificial insemination. Development of multiresistant bacteria is a major concern if mixtures of antibiotics are used in semen extenders. Minimal contamination prevention techniques and surveillance of critical hygiene control points proved to be efficient in reducing bacterial load and preventing development of antibiotic resistance. Nevertheless, novel antimicrobial concepts are necessary for efficient bacterial control in extended boar semen with a minimum risk of evoking antibiotic resistance. Enhanced efforts have been made in recent years in the design and use of antimicrobial peptides (AMPs) as alternatives to conventional antibiotics. The male genital tract harbors a series of endogenic substances with antimicrobial activity and additional functions relevant to the fertilization process. However, exogenic AMPs often exert dose- and time-dependent toxic effects on mammalian spermatozoa. Therefore, it is important that potential newly designed AMPs have only minor impacts on eukaryotic cells. Recently, synthetic magainin derivatives and cyclic hexapeptides were tested for their application in boar semen preservation. Bacterial selectivity, proteolytic stability, thermodynamic resistance, and potential synergistic interaction with conventional antibiotics propel predominantly cyclic hexapeptides into highly promising, leading candidates for further development in semen preservation. The time scale for the development of resistant pathogens cannot be predicted at this moment. PMID:26264695

  7. A long-day light program accelerates seasonal coat changes but is without effect on semen and metabolic parameters in Shetland pony stallions.

    Science.gov (United States)

    Schrammel, Nadine; Deichsel, Katharina; Aurich, Jörg; Aurich, Christine

    2016-03-15

    Horses are seasonal breeders, and robust breeds may exhibit a winter hypometabolism when kept under semiferal conditions. In this study, we analyzed the effects of artificial long days on rectal temperature, heart rate, heart rate variability, hematology, coat changes, semen parameters, and plasma testosterone concentrations in Shetland stallions stabled overnight and assigned to a control group (CON, n = 9) kept under natural photoperiod, and a treatment group exposed to a long-day light program from 15 December to 20 March (AL, n = 9). During the 8-month study, rectal temperature, heart rate, and heart rate variability at no time differed between groups. Plasma total protein (P < 0.05), hematocrit, leukocyte, and lymphocyte counts (P < 0.001) first increased and then decreased during the study period but did not differ between groups. Length of the guard hair decreased over time (P < 0.001) and this decrease occurred earlier in AL than in CON stallions (time × group P < 0.001). Hair regrowth was faster in CON than in AL stallions (over time P < 0.001, time × group P < 0.001). Total sperm count increased from January to April (AL) and May (CON; P < 0.001) but did not differ between groups. Sperm motility and percentage of membrane-intact spermatozoa showed no clear seasonal changes and semen parameters did not differ between groups. In conclusion, Shetland stallions showed seasonal variations in hair coat and total sperm count but only changes in hair coat but not semen parameters were advanced by a long-day light program. PMID:26673622

  8. Evaluation of Physical Semen Characteristics of Male Rabbits Exposed to Different Climatic Conditions and Lighting Regimes Using Nuclear Techniques

    International Nuclear Information System (INIS)

    The number of 20 mature males New Zealand White (NZW) rabbits, in the first production year was used in the present research. The study included two periods; each was of 2 months. The first period was under mild conditions (18.0 degree C) while the second one was during hot conditions (35.0 degree C). In each period, 10 males with the same age and average live body weight were used. Animals within each period were divided randomly into two equal groups, with nearly equal body weights. One of the two groups exposed to natural day light (NDL) which was 10:50 L: 13:10 D in winter and 13:40 L: 10:20 D in summer and was considered as photo period control and the other group was exposed to photo period treatment (Artificial photo period, AP). The treatment group was exposed to artificial long photo period (13:40 L: 10:20 D) during winter and artificial short photo period (10:50 L: 13:10 D) during hot conditions. In seminal plasma, T4, T3 and testosterone hormonal levels were significantly lower in heat stressed rabbits than those reared under mild conditions. In contrast, the hot condition was accompanied by significant increases in cortisol level. T3 and cortisol affected significantly while T4 and testosterone levels were not affected significantly due to change in period of lighting. Concerning physical semen characteristics i.e. ejaculate volume, sperm motility, sperm cell concentration, total sperm output and number of motile sperms per ejaculate were significantly lower under heat stress than under mild conditions. In contrast, hot conditions were accompanied by a significant increase in each of reaction time, dead sperm %, sperm abnormalities % and acrosomal abnormalities %. Exposure of male rabbits during winter to long lighting as compared to NDL caused significant increase in T3 (1.4 vs. 1.3 ng/ml), testosterone (3.2 vs. 2.8 ng/ml) and cortisol (1.8 vs. 1.5 ng/ml) levels as well as significant decline in semen quality, i.e., ejaculate volume (70 vs. 60 x 10-2 ml), sperm motility (76.8 vs. 70.8%), total number sperm-cell output per ejaculate (287.00 vs. 240.00 x106 ) and number of motility sperm output per ejaculate (220.42 vs. 169.92 x106 ). Exposure of male rabbits during summer to short lighting as compared to NDL caused significant increase in T3 (1.10 vs.0.90 ng/ml) and cortisol (2.8 vs. 2.3 ng/ml) in seminal plasma as well as significant decrease in sperm motility (64.8 vs. 60.8%) and significant increase in reaction time (11.6 vs. 12.8 seconds), ejaculate volume (50 vs. 58 x 10-2 ml) and total number sperm-cell output per ejaculate (190.00 vs. 208.80 x 106 ). Finally, correlations between physical semen characteristics and seminal plasma hormonal levels were carried out to evaluate the rabbit bucks semen using nuclear technique

  9. Parental age at delivery and a man's semen quality

    DEFF Research Database (Denmark)

    Priskorn, Lærke; Jensen, Tina K; Lindahl-Jacobsen, Rune; Skakkebæk, Niels E; Bostofte, Erik; Eisenberg, Michael L

    2014-01-01

    with a range of adverse health effects in the offspring. Given the varied health effects of parental age upon offspring, and the sensitivity of genital development to external factors, it is plausible that the age of a man's mother and father at conception may impact his reproductive health. To our......, represent the general population. WIDER IMPLICATIONS OF THE FINDINGS: The current study found no link between parental age and a son's semen quality, suggesting other factors may explain recent impairments in men's reproductive health. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the Hans...... and Nora Buchard's Fund and the Kirsten and Freddy Johansen's Fund. No competing interests. TRIAL REGISTRATION NUMBER: Not relevant....

  10. The Effect of Phyto-Lecithin on Preservation and Cryopreservation of Semen: A Review

    Directory of Open Access Journals (Sweden)

    AS Aku

    2007-01-01

    Full Text Available Artificial insemination represents one of technologies in livestock reproduction that can be applied to cattle, sheep, goats and other livestock. Application of livestock reproduction technology includes artificial insemination to increase reproductive efficiency. Semen processing is one critical phase in an artificial insemination program. The use of animal origin ingredient for semen extenders, such as egg yolk and milk, presents a risk of microbial contamination, which lead to the search for alternatives. To increase standard of quality, researchers exploits phyto-lesitin for semen extender and the results showed no significant differences in motility, viability, and acrosomal status of spermatozoa with phyto-lesitin extender when compared to tris-egg yolk-containing extenders. (Animal Production 9(1: 49-52 (2007 Key Words : Phyto-Lechitin, preservation, cryopreservation, semen

  11. Cryopreservation of tambaqui (Colossoma macropomum) semen: extenders, cryoprotectants, dilution ratios and freezing methods.

    Science.gov (United States)

    Carneiro, P C F; Azevedo, H C; Santos, J P; Maria, A N

    2012-01-01

    The tambaqui is an Amazonian fish of great economic and environmental importance to Brazil and other South American countries. Several semen cryopreservation methodologies have been tested for different Brazilian fish species; however, there is little information on the use of this technique on tambaqui semen. The aim of the present study was to investigate the effect of osmolarity and activation solutions on sperm kinetics and, glucose solutions, cryoprotectants, dilution ratios, egg yolk and freezing methods on tambaqui semen freezing. The osmolarity of 230 mOsm was suitable for simultaneously yielding higher sperm motility (85%) and motility time (54 sec.) and osmolarities above 360 mOsm maintain immobile tambaqui sperm. The tambaqui semen can be successfully cryopreserved when diluted 1:9 in freezing medium composed of 5 percent glucose solution (290 mOsm) with 10 percent methylglycol and 5 percent egg yolk, and frozen directly in a dry shipper container. PMID:23224371

  12. Critical appraisal of conventional semen analysis in the context of varicocele.

    Science.gov (United States)

    Kruger, Thinus

    2016-01-01

    Varicocele is present in approximately 15% of men, and, although it is the most commonly diagnosed cause of male infertility, nearly two-thirds of men with varicoceles remain fertile. It was decided to make use of the current evidence obtained from the previous meta-analyses between 2004 and 2015 as well as available articles covering this field, preferably randomized controlled articles dealing with the topic of semen analysis before and after repair. Two important meta-analyses were discussed as well as other articles dealing with the topic of semen analysis before and after varicocelectomy. The evidence suggests that all semen parameters improve after varicocele repair. Based on the available evidence, it is clear that there is a benefit in treating men with a palpable varicocele. One can expect that all semen parameters will improve within 3 months after repair. PMID:26620460

  13. A novel method for semen collection and artificial insemination in large parrots (Psittaciformes).

    Science.gov (United States)

    Lierz, Michael; Reinschmidt, Matthias; Müller, Heiner; Wink, Michael; Neumann, Daniel

    2013-01-01

    The paper described a novel technique for semen collection in large psittacines (patent pending), a procedure which was not routinely possible before. For the first time, a large set of semen samples is now available for analysis as well as for artificial insemination. Semen samples of more than 100 psittacine taxa were collected and analysed; data demonstrate large differences in the spermatological parameters between families, indicating an ecological relationship with breeding behaviour (polygamous versus monogamous birds). Using semen samples for artificial insemination resulted in the production of offspring in various families, such as Macaws and Cockatoos, for the first time ever. The present technique represents a breakthrough in species conservation programs and will enable future research into the ecology and environmental factors influencing endangered species. PMID:23797622

  14. The Effect of Shelter on Semen Quality of “Peranakan Ettawa” Goat

    Directory of Open Access Journals (Sweden)

    A Qisthon

    2007-05-01

    Full Text Available The experiment was conducted to study the effect of shelter on semen quality of Peranakan Ettawa (PE Goats Eight PE goats were allocated into cross over design. Four PE goats were placed under no shelter (09.00-14.30 and another one was placed under shelter. The results of this research showed that semen volume, sperm motility, sperm concentration, and live sperm percentage of PE goat under shelter were higher (P<0.01 than those of PE goat under no shelter. On the other hand, sperm abnormality of PE goat under shelter was lower (P<0.01 than that of PE goat under no shelter. It was concluded that the use of shelter could improve semen quality. (Animal Production 9(2: 73-78 (2007 Key Words : Shelter, semen, goat

  15. Non-invasive collection and analysis of semen in wild macaques.

    Science.gov (United States)

    Thomsen, Ruth

    2014-04-01

    Assessments of primate male fertility via semen analyses are so far restricted to captivity. This study describes a non-invasive method to collect and analyse semen in wild primates, based on fieldwork with Yakushima macaques (Macaca fuscata yakui). Over nine mating seasons between 1993 and 2010, 128 masturbatory ejaculations were recorded in 21 males of 5 study troops, and in 11 non-troop males. In 55 %, ejaculate volume was directly estimated, and in 37 %, pH-value, sperm vitality, numbers, morphology and swimming velocity could also be determined. This approach of assessing semen production rates and individual male fertility can be applied to other primate taxa, in particular to largely terrestrial populations where males masturbate frequently, such as macaques and baboons. Furthermore, since explanations of male reproductive skew in non-human primate populations have until now ignored the potential role of semen quality, the method presented here will also help to answer this question. PMID:24193510

  16. Semen banking: consideration on viral contamination in the era of new emerging viral infection

    Directory of Open Access Journals (Sweden)

    Viroj Wiwanitkit

    2011-01-01

    Full Text Available To construct a semen bank, the collection of donated semen has to be done and an important concern is the safety of collected semen. The contamination is a big problem. Basically, the infectious pathogens can exist within donated semen, hence, a good donor screening is very important. Although viruses have an indirect role in sperm quality, but the evidence in banked semen is presently lack. This does not mean that there is no viral contamination but it might imply the inadequate concern on this issue. Contaminated semen usually means poor quality and hazardous to the recipient. The contamination of the virus in banked semen is a common problem in animal semen banking (1. The safety and transmission of each problematic virus is widely studied and well clarified in animal semen banking (2. However, this issue is not widely concerned in human semen banking. For sure, this case is an actual direct contamination and this cannot be detected if there is no specific screening in the banking process. The scenario of important new emerging viral infections will be specifically detailed in this report. West Nile virus is an emerging problematic viral infection that can cause a deadly clinical disorder. Basically, West Nile virus is classified as an arbovirus that is mainly transmitted by mosquito. However, the uncommon modes of transmissions such as transfusion related transmission are reported (3. The contamination of West Nile virus in semen is an important question in andrology. There is no evidence indicating for the presence of West Nile virus in the semen of the patients. However, American Society for Reproductive Medicine/Society for Assisted Reproductive Technology recommended that practitioners defer gamete donors who have confirmed or suspected West Nile virus infections (4. SARS is another deadly emerging viral infection. The new coronavirus infection is transmitted via respiratory route. The serious symptom due to this infection leads to death in almost all cases and brings a great concern to medical scientists around the world. The contamination of SARS in semen is an interesting topic. The possible transmission of SARS virus via germ line is an important question to be investigated in reproductive medicine (5. Luckily, till present, there is no evidence of SARS contamination in semen. Generally, influenza virus is a respiratory virus that causes respiratory tract infection. In the recent few years, an atypical influenza, avian flu, emerged. This infection brought a concern to the medical society. In early of this year, 2009, the newest emerging viral infection caused by a novel influenza virus, swine flu occurred and became pandemic. The topic on the new influenza virus becomes the present hot issue. Focusing on the contamination of classical influenza virus in semen, there are many evidences confirming the existence of virus in semen derived from the infected cases. It is also

  17. HPV Prophylactic Vaccination in Males Improves the Clearance of Semen Infection

    Directory of Open Access Journals (Sweden)

    Carlo Foresta

    2015-10-01

    Discussion: Humoral immunity has a major role in healing from HPV infection. Elder ART patients with HPV semen infection may benefit by the union of both specific counselling and available prophylactic vaccination.

  18. Effects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspius

    Directory of Open Access Journals (Sweden)

    Saeed Hajirezaee

    2009-01-01

    Full Text Available Problem statement: Because of dramatic declines in stocks of endangered Caspian brown trout males, Salmo trutta caspius in Caspian Sea, each male brooder is stripped indispensably more than once during the spawning season in other to artificial insemination in hatchery. The aim of the present study was to assay the changes of indicators of semen quality (sperm motility, sperm production, semen volume and chemical composition of seminal fluid during these sequential strippings. Approach: The 11 tagged males were stripped four times every 12-14 days with beginning of spermiation period (2 December 2008 towards its end (10 January 2008. One-way Analysis Of Variance (ANOVA was employed to analyze differences between means of semen parameters. Also, the relationships between semen parameters were tested using the bivariate correlation coefficients of Pearson. Results: The semen volume, sperm density, osmolality and the concentrations of Na+, Cl-, K+, Ca2+, Mg2+ and total protein gradually decreased whereas the values of glucose and triglyceride had no significant changes during sequential strippings. Also, the values of semen pH, the percentage (5s post-activation and duration of motility were statistically stable until third stripping but a decrease was recorded for these parameters in the fourth stripping. As well as, significant positive correlations were found for sperm density vs. K+, Cl-, Na+, Ca2+, Mg2+, total protein, spermatocrit; the percentage of motile spermatozoa Vs Ca2+, Mg2+, K+, Cl-, Na+, total protein and also the duration of motility Vs K+, Cl-, total protein and pH. Conclusion: The semen quality of Caspian brown trout males decrease in successive strippings during spawning season. Also, the knowledge on values and correlations between the sperm motility characteristics and the composition of seminal fluid could be useful to formulation of a species-specific extender solution for cryopreservation of semen of Caspian brown trout.

  19. The Effect of Phyto-Lecithin on Preservation and Cryopreservation of Semen: A Review

    OpenAIRE

    AS Aku; N Sandiah; PD Sadsoeitoeboen; Amin, R.; Herdis .

    2007-01-01

    Artificial insemination represents one of technologies in livestock reproduction that can be applied to cattle, sheep, goats and other livestock. Application of livestock reproduction technology includes artificial insemination to increase reproductive efficiency. Semen processing is one critical phase in an artificial insemination program. The use of animal origin ingredient for semen extenders, such as egg yolk and milk, presents a risk of microbial contamination, which lead to the search f...

  20. The Association between Dietary Patterns and Semen Quality in a General Asian Population of 7282 Males

    OpenAIRE

    Liu, Chin-Yu; Chou, Yu-Ching; Chao, Jane C. -J.; Hsu, Chien-Yeh; CHA, TAI-LUNG; Tsao, Chih-Wei

    2015-01-01

    Objective To explore the associations between different dietary patterns and semen quality in a general Asian male population. Methods Cross-sectional study. Healthy Taiwanese men aged 18 years or older who participated in a standard medical screening program from 2008-2013 run by a private firm were included in this study. Semen parameters including sperm concentration (SC), total sperm motility (TSM), progressive motility (PRM) and normal sperm morphology (NSM) were recorded. A dietary ques...

  1. Comparisons of antibiotic combinations to control Pseudomonas aeruginosa in bovine semen.

    OpenAIRE

    EAGLESOME, M.D.; Garcia, M M

    1995-01-01

    Raw semen experimentally contaminated with 10(6) Pseudomonas aeruginosa cells per milliliter was processed for use in artificial insemination (AI) using three different antibiotic combinations: a) gentamicin, lincomycin, spectinomycin and tylosin (GLST) directly added to contaminated raw semen followed by dilution with whole milk or egg yolk Tris containing GLST; b) penicillin, streptomycin, lincomycin, spectinomycin and minocycline (PSLSM) in whole milk used to dilute the contaminated raw se...

  2. Semen quality in papaya workers with long term exposure to ethylene dibromide.

    OpenAIRE

    Ratcliffe, J. M.; Schrader, S M; Steenland, K; Clapp, D E; Turner, T.; Hornung, R W

    1987-01-01

    To examine whether long term occupational exposure to ethylene dibromide (EDB) affects semen quality a cross sectional study of semen quality was conducted among 46 men employed in the papaya fumigation industry in Hawaii, with an average duration of exposure of five years and a geometric mean breathing zone exposure to airborne EDB of 88 ppb (eight hour time weighted average) and peak exposures of up to 262 ppb. The comparison group consisted of 43 unexposed men from a nearby sugar refinery....

  3. Semen quality and fertility after artificial insemination in dairy cattle and pigs

    OpenAIRE

    Alm-Packalén, Karoliina

    2009-01-01

    The economic importance of a high breeding efficiency in sows and dairy cows emphasizes the benefit of accurate prediction of fertility of boar and bull semen. The artificial insemination (AI) studs need an objective and rapid, but inexpensive, method to evaluate ejaculates. We developed a new quick and easy fluorescence method for frozen-thawed bull semen that uses an automatized fluorometer and the fluorophore stain propidium iodide that stains only cells with damaged membranes. The fluores...

  4. Effects of Thawing on Frozen Semen Quality of Limousin and Brahman Bulls

    OpenAIRE

    wc Pratiwi; L Affandhy; D Ratnawati

    2009-01-01

    The success of Artificial Insemination (AI )influenced by many factor, there are nutrition, body condition and post thawing motility (PTM). The PTM influenced by liquid N2 storage, equilibration temperature and handling straw. The purpose of this research to compare the effect of thawing duration to frozen semen quality of Limousin and Brahman. This research was done in BIBD, Agriculture Official of Blora, Central Java and Laboratory of Beef Cattle Station Research, Grati. As semen source is ...

  5. Duration of Ebola virus RNA persistence in semen of survivors: population-level estimates and projections.

    Science.gov (United States)

    Eggo, Rosalind M; Watson, Conall H; Camacho, Anton; Kucharski, Adam J; Funk, Sebastian; Edmunds, W John

    2015-12-01

    Ebola virus can persist in semen after recovery, potentially for months, which may impact the duration of enhanced surveillance required after interruption of transmission. We combined recent data on viral RNA persistence with weekly disease incidence to estimate the current number of semen-positive men in affected West African countries. We find the number is low, and since few reported sexual transmission events have occurred, the future risk is also likely low, although sexual health promotion remains critical. PMID:26676163

  6. Analysis of breed effects on semen traits in light horse, warmblood, and draught horse breeds.

    Science.gov (United States)

    Gottschalk, Maren; Sieme, Harald; Martinsson, Gunilla; Distl, Ottmar

    2016-05-01

    In the present study, systematic effects on semen quality traits were investigated in 381 stallions representing 22 breeds. All stallions were used for AI either at the Lower Saxon National Stud Celle or the North Rhine-Westphalian National Stud Warendorf. A total of 71,078 fresh semen reports of the years 2001 to 2014 were edited for analysis of gel-free volume, sperm concentration, total number of sperm, progressive motility, and total number of progressively motile sperm. Breed differences were studied for warmblood and light horse breeds of both national studs (model I) and for warmblood breeds and the draught horse breed Rhenish German Coldblood from the North Rhine-Westphalian National stud (model II) using mixed model procedures. The fixed effects of age class, year, and month of semen collection had significant influences on all semen traits in both analyses. A significant influence of the horse breed was found for all semen traits but gel-free volume in both statistical models. Comparing warmblood and light horse stallions of both national studs, we observed highest sperm concentrations, total numbers of sperm, and total numbers of progressively motile sperm in Anglo-Arabian stallions. The draught horse breed Rhenish German Coldblood had the highest least squares means for gel-free volume, whereas all other investigated semen traits were significantly lower in this breed compared to the warmblood stallions under study. The variance components among stallions within breeds were significant for all semen traits and accounted for 40% to 59% of the total variance. The between-breed-variance among stallions was not significant underlining the similar size of the random stallion effect in each of the horse breeds analyzed here. In conclusion, breed and stallion are accounting for a significant proportion of the variation in semen quality. PMID:26893165

  7. The Effect of Shelter on Semen Quality of “Peranakan Ettawa” Goat

    OpenAIRE

    A Qisthon; S Suharyati

    2007-01-01

    The experiment was conducted to study the effect of shelter on semen quality of Peranakan Ettawa (PE) Goats Eight PE goats were allocated into cross over design. Four PE goats were placed under no shelter (09.00-14.30) and another one was placed under shelter. The results of this research showed that semen volume, sperm motility, sperm concentration, and live sperm percentage of PE goat under shelter were higher (P

  8. Cadmium, Chromium, and Copper Concentration plus Semen-Quality in Environmental Pollution Site, China.

    OpenAIRE

    Yan Li; Qiaoyan Gao; Mingcai Li; Mengyang Li; Xueming Gao

    2014-01-01

    The environmental pollution is one of the factors contributing to the decrease of sperm quality for human beings. The aim of this study was to assess cadmium (Cd), chromium (Cr), and copper (Cu) concentration of man in environmental pollution site, and explore relationships between men exposure to Cd, Cr, and Cu and semen-quality parameters in environmental pollution site.Ninety five men were recruited through pollution area and controls in 2011. We measured semen quality using Computer-aided...

  9. PERCEPATAN PROSES PRODUKSI DAN PENINGKATAN KUALITAS PAPAN SEMEN KOMPOSIT BERBAHAN BAKU BAMBU MELALUI INJEKSI KARBONDIOKSIDA

    OpenAIRE

    Bakri; Musrizal Muin; Baharuddin; Sahriyanti Saad1

    2014-01-01

    Injeksi CO2 pada fase dan waktu tertentu merupakan gagasan yang relatif baru untuk meningkatkan sifat fisik dan mekanik papan semen. Penelitian sebelumnya menunjukkan bahwa injeksi CO2 pada fase cair dan waktu injeksi 60 menit merupakan perlakuan terbaik untuk meningkatkan sifat fisik dan mekanik papan semen yang dibuat dari campuran partikel batang bamboo tallang (Gigantochloa atter). Beberapa peneliti menemukan bahwa penambahan bahan-bahan aditif pada campuran juga dapat meningkatkan sifat ...

  10. Biokompatibilitas semen ronomer kaca terhadap kehidupan jaringan gigi merupakan pertimbangan utama dalam pemakaiannya sebagai basis pada restorasi sandwich. Ion fluor yang dikandung semen ionomcr kaca mcrupakan unsur kimia paling penting yang diharapkan b

    OpenAIRE

    Irene Rismauli Manurung

    2008-01-01

    Biokompatibilitas semen ronomer kaca terhadap kehidupan jaringan gigi merupakan pertimbangan utama dalam pemakaiannya sebagai basis pada restorasi sandwich. Ion fluor yang dikandung semen ionomcr kaca mcrupakan unsur kimia paling penting yang diharapkan banyak diikat oleh email. Semen ionomer kaca mempunyai sifat anti kariogenik dan anti mikrobial karena adanya ion fluor yang dilepaskan seeara teratur dan terus menerus. Fluor mengurangi produksi asam yang dihasilkan oleh mikroorganisme d...

  11. Preservasi Semen Kambing Peranakan Etawa dalam Pengencer Tris dan Sitrat Kuning Telur dengan Penambahan Sodium Dodecyl Sulphate (THE PRESERVATION OF ETTAWA GRADE BUCK SEMEN IN TRIS AND CITRATE EGG YOLK DILUENTS SUPPLEMENTED WITH SODIUM DODECYL SULPHATE)

    OpenAIRE

    Nur Hidayati; Raden Iis Arifiantini; Dondin Sajuthi3)

    2015-01-01

    This study was conducted to determineSDS concentration also to compare Tris egg yolk and citrateegg yolk on the quality of ettawa grade chilled semen. The study consist of two experiments. The firstexperiment was to determine the best SDS concentration in Tris egg yolk diluents and the second experimentwas to compare the SDS suplementation in tris and citrate egg yolk in the quality of ettawa grade chilledsemen. The semen were collected from three bucks, immediately after collection the semen...

  12. PERANAN BETA-KAROTEN DALAM MEMPERTAHANKAN DAYA HIDUP SPERMATOZOA SEMEN CAIR DOMBA GARUT ROLE OF BETA-CAROTENE INI MAINTAINING SPERM LIVABILITY OF CHILLED-SEMEN OF GARUT RAM

    Directory of Open Access Journals (Sweden)

    Muhammad Rizal

    2006-12-01

    Full Text Available Tujuan penelitian ini adalah untuk mengevaluasi kualitas semen cair domba garut yang diencerkan dengan larutan pengencer tris dengan penambahan berbagai konsentrasi beta-karoten. Semen dikoleksi dengan vagina buatan satu kali dalam satu minggu dari empat pejantan domba garut dewasa kelamin. Segera setelah dievaluasi, semen segar dibagi ke dalam tiga buah tabung reaksi dan masing-masing diencerkan dengan pengencer tris (kontrol, pengencer tris + 0,0125% beta-karoten

  13. Evaluation of the microbial quality of fresh ejaculates of camel (Camelus dromedarius) semen.

    Science.gov (United States)

    Ghoneim, I M; Waheed, M M; Al-Hofofi, A N; Fayez, M M; Al-Eknah, M M; Al-Busadah, K A; Al-Humam, N A

    2014-10-01

    A series of five factorial arranged experiments were conducted to investigate the effect of different management during semen collection on the microbial quality (bacterial load, type of microbes and frequency of isolation) of dromedaries' semen. Microbial analysis of seventy-nine fresh ejaculates from twenty-two camels showed the presence of nine variant colonies. The most prevalent organisms in the dromedary semen were species of Staphylococcus, Bacillus and Streptococcus. One yeast species was represented among the isolates. The middle aged camels (9-13 years) had significantly (P>0.05) higher mean bacterial loads than young (4-8 years) and old aged (14-18 years) animals. The mean bacterial populations of ejaculates collected by an electro-ejaculator were significantly (P>0.05) higher than those collected by an artificial vagina. Candida spp. was identified in 53.8% of the samples collected by an electro-ejaculator and was not detected in ejaculates collected by an artificial vagina. The mean semen bacterial load detected during the breeding season was significantly (Psemen samples collected in the non-breeding season. The difference between the mean semen bacterial loads in the first and the second ejaculate was highly significant (Psemen is found in different intensities during different management procedures of semen collection. PMID:25132210

  14. Escitalopram treatment for premature ejaculation has a negative effect on semen parameters.

    Science.gov (United States)

    Koyuncu, H; Serefoglu, E C; Yencilek, E; Atalay, H; Akbas, N B; Sarıca, K

    2011-01-01

    The aim of this study was to determine the impact of long-term escitalopram treatment on semen parameters of patients with lifelong premature ejaculation (PE). Between November 2008 and January 2010, patients admitted to urology outpatient clinic with a self-reported complaint of PE were evaluated. Medical and sexual history of patients were recorded and patients with lifelong PE (a total of 25 patients) who met the International Society of Sexual Medicine definition were asked to record their intravaginal ejaculatory latency time (IELT) for 1 month, complete Premature Ejaculation Diagnostic Tool (PEDT) questionnaire and give semen samples. Afterwards, patients received 10 mg escitalopram daily for 12 weeks and were invited for control visits at first and third month of treatment. During control visits, PEDT was administered again whereas IELTs were recorded and semen samples were re-examined. PEDT scores, arithmetic means of IELTs and results of semen analyses, which were recorded at baseline, first and third month were compared. At the third month of treatment, a significant increase in mean IELTs and a significant decrease in PEDT scores were detected. However there was a significant decrease in sperm concentration, motility and morphology when compared with the baseline semen measures. Daily escitalopram treatment effects the semen parameters of patients with lifelong PE. Further investigations with larger series are needed to see whether other serotonin reuptake inhibitors have similar side effects and to expose the exact mechanism underlying it. Different treatment modalities should be suggested to patients who desire fertility. PMID:21776003

  15. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki: A preliminary study

    Directory of Open Access Journals (Sweden)

    M.S. Khairiah

    2012-10-01

    Full Text Available The Malayan gaur (Bos gaurus hubbacki or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN. The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM and electroejaculation (EEJ technique was applied in semen collection of the Malayan gaur. The semen was then cryopreserved in liquid nitrogen using slow freezing technique. Makler counting chamber was used to evaluate sperm concentration and motility, while the sperm viability and morphology of fresh and post-thaw sperm was determined using eosin-nigrosin staining protocol. As a result, we have successfully collected the Malayan gaur semen using EEJ technique. Sperm motility, viability and morphological changes of the post-thaw semen of Malayan gaur were found undesirable due to the complication of the cryopreservation process. On the basis of current study it can be concluded that Malayan gaur bulls semen can be obtain by EEJ with no evidence of rectal trauma. Optimization of the process of cryopreservation for Malayan gaur sperm is needed to maintain the cryoviability of the good sperm quality. The data generated in this study would be useful in conservation of genetic diversity program for Malayan gaur.

  16. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study.

    Science.gov (United States)

    Iswadi, M I; Ann, Z F; Hafiz, M M; Hafiz, M D; Fahrul, F J; Hajarian, H; Wahid, H; Zawawi, I; Khairiah, M S; Mazni, O A

    2012-01-01

    The Malayan gaur (Bos gaurus hubbacki) or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN). The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM) and electroejaculation (EEJ) technique was applied in semen collection of the Malayan gaur. The semen was then cryopreserved in liquid nitrogen using slow freezing technique. Makler counting chamber was used to evaluate sperm concentration and motility, while the sperm viability and morphology of fresh and post-thaw sperm was determined using eosin-nigrosin staining protocol. As a result, we have successfully collected the Malayan gaur semen using EEJ technique. Sperm motility, viability and morphological changes of the post-thaw semen of Malayan gaur were found undesirable due to the complication of the cryopreservation process. On the basis of current study it can be concluded that Malayan gaur bulls semen can be obtain by EEJ with no evidence of rectal trauma. Optimization of the process of cryopreservation for Malayan gaur sperm is needed to maintain the cryoviability of the good sperm quality. The data generated in this study would be useful in conservation of genetic diversity program for Malayan gaur. PMID:26623302

  17. Detection of Toxoplasma gondii DNA in fresh and frozen semen from rams in Brazil.

    Science.gov (United States)

    Bezerra, M J G; Cruz, J A L O; Kung, E S; Albuquerque, P P F; Kim, P C P; Moraes, E P B X; Pinheiro Júnior, J W; Mota, R A

    2014-10-01

    The aim of this study was to estimate the prevalence of genomic DNA of Toxoplasma gondii in semen samples from commercial rams in artificial insemination centres in Brazil, as well as in fresh semen from rams in the northeast of Brazil. In total, 108 semen samples were obtained from artificial insemination centres, and genomic DNA of T. gondii was detected in 24 of 108 (22.2%). The prevalence of antibodies anti-Toxoplasma gondii among sheep on rural properties was 9.2% (10/109), and 100% of the semen samples of these animals were positive in the PCR for T. gondii DNA. The molecular identity was confirmed through sequencing, which indicated 99.9% similarity with the T. gondii DNA sequences stored in the GenBank. This study reports the first occurrence of T. gondii DNA in the semen of rams, which came from artificial insemination centres in Brazil, as well as the occurrence of T. gondii DNA in the fresh semen of naturally infected rams in the northeast of Brazil. PMID:25130195

  18. Use of commercial extenders and alternatives to prevent sperm agglutination for cryopreservation of brown bear semen.

    Science.gov (United States)

    Gomes-Alves, S; Alvarez, M; Nicolas, M; Lopez-Urueña, E; Martínez-Rodríguez, C; Borragan, S; de Paz, P; Anel, L

    2014-08-01

    The objective of this study was to evaluate different bovine and canine commercial semen extenders for cryopreservation of brown bear ejaculates and the effect of semen collection directly into extender on sperm agglutination. Semen samples were obtained by electroejaculation from 13 adult males. In experiment 1, eleven ejaculates from eight bears were used to evaluate Bioxcell and Andromed as extenders, whereas in experiment 2, nine ejaculates from six bears were used to evaluate Triladyl canine, CaniPro, and Extender 2 as extenders. An extender specifically developed for brown bears (Test-Tris-fructose-egg yolk-glycerol, TTF-ULE/bear) served as a control extender in both experiments. After thawing, total and progressive sperm motility and sperm viability were greater (P Canine, CaniPro, and Extender 2 in experiment 2. In experiment 3, addition of handling extender (TTF-H) to the semen collection tube for eight ejaculates from seven bears resulted in less (P sperm agglutination in fresh samples (score 0.5 ± 0.2 vs. 1.8 ± 0.4 in diluted and control samples, respectively) with no effect on pre-freeze and post-thawing semen quality. In conclusion, TTF-ULE/bear is the most suitable extender for brown bear semen cryopreservation, but comparable results can be obtained with the commercial extender Andromed. In addition, collection of ejaculates directly in TTF-H extender decreases sperm agglutination in fresh samples. PMID:24950618

  19. Urinary inorganic arsenic concentrations and semen quality of male partners of subfertile couples in Tokyo.

    Science.gov (United States)

    Oguri, Tomoko; Yoshinaga, Jun; Toshima, Hiroki; Mizumoto, Yoshifumi; Hatakeyama, Shota; Tokuoka, Susumu

    2016-05-11

    Inorganic arsenic (iAs) has been known as a testicular toxicant in experimental rodents. Possible association between iAs exposure and semen quality (semen volume, sperm concentration, and sperm motility) was explored in male partners of couples (n = 42) who visited a gynecology clinic in Tokyo for infertility consultation. Semen parameters were measured according to WHO guideline at the clinic, and urinary iAs and methylarsonic acid (MMA), and dimethylarsinic acid concentrations were determined by liquid chromatography-hydride generation-ICP mass spectrometry. Biological attributes, dietary habits, and exposure levels to other chemicals with known effects on semen parameters were taken into consideration as covariates. Multiple regression analyses and logistic regression analyses did not find iAs exposure as significant contributor to semen parameters. Lower exposure level of subjects (estimated to be 0.5 μg kg(-1) day(-1)) was considered a reason of the absence of adverse effects on semen parameters, which were seen in rodents dosed with 4-7.5 mg kg(-1). PMID:26865228

  20. Presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men.

    Science.gov (United States)

    Filipiak, E; Marchlewska, K; Oszukowska, E; Walczak-Jedrzejowska, R; Swierczynska-Cieplucha, A; Kula, K; Slowikowska-Hilczer, J

    2015-09-01

    Urogenital tract infections in males are one of the significant etiological factors in infertility. In this prospective study, 72 patients with abnormal semen parameters or any other symptoms of urogenital tract infection were examined. Semen analysis according to the WHO 2010 manual was performed together with microbial assessment: aerobic bacteria culture, Chlamydia antigen test, Candida culture, Ureaplasma and Mycoplasma-specific culture. In total, 69.4% of semen samples were positive for at least one micro-organism. Ureaplasma sp. was the most common micro-organism found in 33% of semen samples of infertile patients with suspected male genital tract infection. The 2nd most common micro-organisms were Enterococcus faecalis (12.5%) and Escherichia coli (12.5%), followed by Staphylococcus aureus (7%), Chlamydia trachomatis (7%) and Candida sp. (5.6%). Generally, bacteria were sensitive to at least one of the antibiotics tested. No statistically significant relationship was observed between the presence of aerobic micro-organisms in semen and basic semen parameters: volume, pH, concentration, total count, motility, vitality and morphology. PMID:25209133

  1. Semen parameters can be predicted from environmental factors and lifestyle using artificial intelligence methods.

    Science.gov (United States)

    Girela, Jose L; Gil, David; Johnsson, Magnus; Gomez-Torres, María José; De Juan, Joaquín

    2013-04-01

    Fertility rates have dramatically decreased in the last two decades, especially in men. It has been described that environmental factors as well as life habits may affect semen quality. In this paper we use artificial intelligence techniques in order to predict semen characteristics resulting from environmental factors, life habits, and health status, with these techniques constituting a possible decision support system that can help in the study of male fertility potential. A total of 123 young, healthy volunteers provided a semen sample that was analyzed according to the World Health Organization 2010 criteria. They also were asked to complete a validated questionnaire about life habits and health status. Sperm concentration and percentage of motile sperm were related to sociodemographic data, environmental factors, health status, and life habits in order to determine the predictive accuracy of a multilayer perceptron network, a type of artificial neural network. In conclusion, we have developed an artificial neural network that can predict the results of the semen analysis based on the data collected by the questionnaire. The semen parameter that is best predicted using this methodology is the sperm concentration. Although the accuracy for motility is slightly lower than that for concentration, it is possible to predict it with a significant degree of accuracy. This methodology can be a useful tool in early diagnosis of patients with seminal disorders or in the selection of candidates to become semen donors. PMID:23446456

  2. Individual variation related to testicular biometry and semen characteristics in collared peccaries (Tayassu Tajacu Linnaeus, 1758).

    Science.gov (United States)

    Peixoto, G C X; Silva, M A; Castelo, T S; Silva, A M; Bezerra, J A B; Souza, A L P; Oliveira, M F; Silva, A R

    2012-10-01

    The aim of this research was to study the individual variation with regard to the morphometry of the testes evaluated by ultrasonography and semen characteristics and to verify the existence of relationship between these variables in collared peccaries. In addition, the testes of the animals were evaluated by histology in order to determine the proportion occupied by the seminiferous tubules. A total of 52 ejaculates were obtained from ten adult specimens that had been restrained by anesthesia. The testicular measurements (length, height, and width) were performed by ultrasonography, and the testicular volume was calculated according to Lambert's formula. The scrotal circumference was measured by encircling the thickest portion of the testicle with a graduated nylon tape. The semen was collected by electroejaculation. Testicular fragments were analyzed through classic histology for the determination of the area occupied by the seminiferous tubules. The results show a great amount of individual variation with regard to testicular morphometry and semen characteristics. No significant correlations were obtained between testicular measurements and semen characteristics. The histometric analysis revealed that 67.8% of the testes are occupied by seminiferous tubules. Results show that the measurement of testicular dimensions does not serve as an indicator of the quality of semen obtained by electroejaculation in collared peccaries, as there is no correlation between testicular morphometry and semen characteristics in this species that presents large variations among individuals. PMID:22964034

  3. Reproductive toxicity of chromium in adult bonnet monkeys (Macaca radiata Geoffrey). Reversible oxidative stress in the semen

    International Nuclear Information System (INIS)

    The present study was designed to test the hypothesis that oxidative stress mediates chromium-induced reproductive toxicity. Monthly semen samples were collected from adult monkeys (Macaca radiata), which were exposed to varying doses (50, 100, 200 and 400 ppm) of chromium (as potassium dichromate) for 6 months through drinking water. Chromium treatment decreased sperm count, sperm forward motility and the specific activities of antioxidant enzymes, superoxide dismutase and catalase, and the concentration of reduced glutathione in both seminal plasma and sperm in a dose- and duration-dependent manner. On the other hand, the quantum of hydrogen peroxide in the seminal plasma/sperm from monkeys exposed to chromium increased with increasing dose and duration of chromium exposure. All these changes were reversed after 6 months of chromium-free exposure period. Simultaneous supplementation of vitamin C (0.5 g/L; 1.0 g/L; 2.0 g/L) prevented the development of chromium-induced oxidative stress. Data support the hypothesis and show that chronic chromium exposure induces a reversible oxidative stress in the seminal plasma and sperm by creating an imbalance between reactive oxygen species and antioxidant system, leading to sperm death and reduced motility of live sperm

  4. Trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in Nili Ravi buffaloes (Bubalus bubalis).

    Science.gov (United States)

    Iqbal, Sajid; Andrabi, Syed Murtaza Hassan; Riaz, Amjad; Durrani, Aneela Zameer; Ahmad, Nasim

    2016-03-15

    Our objectives were to study the effect of trehalose in extender on (1) antioxidant enzymes profile during cryopreservation (after dilution, before freezing, and after thawing), (2) invitro quality (after thawing), and (3) invivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n=20) from four buffalo bulls were diluted in Tris-citric acid-based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60mM) and frozen in French straws. At post dilution, profile of sperm catalase (U/mL) was higher (P<0.05) in extenders containing 15, 30, and 45mM of trehalose as compared to control. Although profiles of superoxide dismutase (U/mL) and total glutathione (?M) were higher (P<0.05) in extenders containing 15 and 30mM of trehalose as compared to control. At prefreezing, sperm catalase, superoxide dismutase, and total glutathione profiles were higher (P<0.05) in all the treatment groups as compared to control. At post thawing, the profiles of catalase and total glutathione were higher (P<0.05) in extender containing 30-mM trehalose as compared to other treatment groups and control. Whereas, profile of superoxide dismutase was higher (P<0.05) in extenders containing 30, 45, and 60mM of trehalose as compared to control and 15mM group. Post thaw total sperm motility (%) was higher (P<0.05) in extender containing 30-mM trehalose as compared to control and 15 and 60-mM groups. Although sperm progressive motility (%), rapid velocity (%), average path velocity (?m/s), straight line velocity (?m/s), curvilinear velocity (?m/s), plasma membrane (structural and functional, %), acrosome (%), and DNA (%) integrity were higher (P<0.05) in extender containing 30mM trehalose as compared to other treatment groups and control. The fertility rates (61% vs. 43%) were higher (P<0.05) in buffaloes inseminated with semen doses cryopreserved in extender containing 30mM of trehalose than the control. It is concluded that addition of 30-mM trehalose in extender improves the semen antioxidant enzymes activity, post thaw quality, and fertility in Nili Ravi buffaloes. PMID:26653954

  5. Uso de la prueba hipoosmótica en la evaluación de la fertilidad potencial de semen canino fresco y congelado Evaluation of fresh and frozen canine semen by the hypoosmotic swelling test

    OpenAIRE

    A. SANCHEZ; J. Rubilar; R. GATICA

    2002-01-01

    Se estudió la integridad de la membrana espermática en espermatozoides caninos en semen fresco y en semen congelado/descongelado, mediante la prueba hipoosmótica y las correlaciones de dicha prueba con algunos parámetros de evaluación usados de rutina en el estudio de semen de perro, usando 20 eyaculados. El semen se obtuvo por manipulación digital. A través de un espermiograma convencional se obtuvo un volumen promedio 1.9 ± 0.9 ml (1ª y 2ª fracciones), 83.8% de motilidad progresiva, 92.0% d...

  6. Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE)

    OpenAIRE

    Oriza Savitri Ariantie; Tuty Laswardi Yusuf; Dondin Sajuthi3); Raden Iis Arifiantini

    2014-01-01

    The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C...

  7. A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men

    DEFF Research Database (Denmark)

    Vested, Anne; Ramlau-Hansen, Cecilia H; Bonde, Jens P; Thulstrup, Ane M; Kristensen, Susanne L; Toft, Gunnar

    2011-01-01

    ) using semen samples from 166 young Danish men. The CRISMAS software identifies sperm concentration and classifies spermatozoa into three motility categories. To enable comparison of the two methods, the four motility stages obtained by conventional semen analysis were, based on their velocity...... proportion of rapidly progressive spermatozoa and, consequently, underestimated the percentages of slowly progressive and non-progressive spermatozoa, compared to the conventional method. To investigate whether results drifted according to time of semen analysis, results were pooled into quarters according...

  8. Impact of porcine circovirus type 2 (PCV2) vaccination on boar semen quality and quantity using two different vaccines

    OpenAIRE

    Caspari, K; Henning, H.; Schreiber, F; Maass, P.; Gössl, R; Schaller, C.; Waberski, D

    2014-01-01

    Porcine circovirus type-2 (PCV2) is widespread in domestic pig populations. It can be shed with boar semen, but the role boars have in epidemiology is still unclear. Vaccinating boars against PCV2 can reduce disease and virus load in semen, but may have unwanted side effects, that is, impairment of spermatogenesis. Therefore, the aim of this study was to investigate the effect and impact of two different PCV2 vaccines on boar semen quality and quantity. Healthy normospermic Large White boars ...

  9. Cadmium, Lead, and Other Metals in Relation to Semen Quality: Human Evidence for Molybdenum as a Male Reproductive Toxicant

    OpenAIRE

    Meeker, John D.; Rossano, Mary G.; Protas, Bridget; Diamond, Michael P.; Puscheck, Elizabeth; Daly, Douglas; Paneth, Nigel; Wirth, Julia J.

    2008-01-01

    Background Evidence on human semen quality as it relates to exposure to various metals, both essential (e.g., zinc, copper) and nonessential (e.g., cadmium, lead), is inconsistent. Most studies to date used small sample sizes and were unable to account for important covariates. Objectives Our goal in this study was to assess relationships between exposure to multiple metals at environmental levels and human semen-quality parameters. Methods We measured semen quality and metals in blood (arsen...

  10. Human semen quality in the new millennium: a prospective cross-sectional population-based study of 4867 men

    DEFF Research Database (Denmark)

    Jørgensen, N; Joensen, Ulla Nordström; Jensen, TK; Jensen, MB; Almstrup, Kristian; Olesen, IA; Juul, A; Andersson, A; Carlsen, E; Petersen, Jørgen Holm; Toppari, J; Skakkebæk, Niels Erik

    2012-01-01

    Objectives Considerable interest and controversy over a possible decline in semen quality during the 20th century raised concern that semen quality could have reached a critically low level where it might affect human reproduction. The authors therefore initiated a study to assess reproductive...... semen quality among young men of the general population showed an increasing trend in sperm concentration and total sperm count. However, only one in four men had optimal semen quality. In addition, one in four will most likely face a prolonged waiting time to pregnancy if they in the future want to...

  11. Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

    Directory of Open Access Journals (Sweden)

    Guilherme Pugliesi

    2012-12-01

    Full Text Available Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender or a glycine egg yolk-based extender (Foote extender and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306. The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.

  12. Effect of split ejaculation and seminal extenders on longevity of donkey semen preserved at 5° C

    OpenAIRE

    Mello S.L.V.; Henry M; Souza M.C.; Oliveira S.M.P.

    2000-01-01

    The aim of this study was to evaluate the longevity of donkey sperm comparing the rich seminal fraction and the whole semen in two extenders, Kenney and modified Baken extenders. Semen of five donkeys were collected through an open-end artificial vagina once a week for five consecutive weeks. The two first jets (rich fraction) of semen were collected separately from the rest of the ejaculate. Whole semen samples were obtained mixing proportionally part of the rich with part of the poor semina...

  13. Fatores de risco na transmissão do lentivírus caprino pelo sêmen Risk factors in caprine lentivirus transmission through semen

    Directory of Open Access Journals (Sweden)

    Alice Andrioli

    2006-08-01

    Full Text Available O objetivo deste trabalho foi avaliar a presença do DNA pró-viral do lentivírus caprino (LVC em ejaculados de machos infectados naturalmente, e verificar a influência da lavagem do sêmen e da presença de inflamação testicular sobre a carga viral. Foram realizadas oito coletas de sêmen de sete reprodutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, o ejaculado era lavado, para retirada do plasma seminal, e na outra, não. O DNA pró-viral do LVC foi identificado pela reação em cadeia da polimerase Nested (PCR Nested, e pelo isolamento viral. O vírus foi isolado em 7,1% das amostras. A PCR identificou o DNA pró-viral em 35,7% do total das amostras: 17,9% nas amostras lavadas e 53,6% das amostras de sêmen integrais. O dano ao testículo permite maior fluxo do vírus para o sêmen, pois antes do dano, 21,4% das amostras foram positivas e pós-dano, 50%. A transmissão do LVC pelo sêmen de reprodutores caprinos é potencializada pela presença de inflamações testiculares e pelo fato de o sêmen criopreservado conter o LVC na forma infectante.The objective of this work was to evaluate the presence of the DNA provirus of the caprine lentivirus (LVC in ejaculates of naturally infected males, and to verify the influence of the wash of the semen as well as the presence of testicle inflammation on the viral load. Eight semen collections of seven soropositive reproducers were accomplished, four before testicle injury and four after injury. Amongst the collections carried out at the same week, in one the ejaculate was washed, to withdraw the plasma seminal, and in the other it was not. The provirus DNA was identified both by Nested polymerase chain reaction technique (Nested PCR and by the viral isolation. The virus was isolated in 7.1% of the samples. The PCR identified the provirus DNA in 35.7% of all samples, 17.9% in the washed samples and 53.6% of the integral semen samples. The injury of the testicle tends to greater flow of virus for the semen, therefore, before injury, 21.4% of the samples were positive and after-injury, 50%. Risk of transmission of the LVC by semen of goat reproducers is strengthened by the presence of testicle inflammations and the fact that the criopreserved semen contains the LVC in infecting form.

  14. Boar semen bacterial contamination in Italy and antibiotic efficacy in a modified extender

    Directory of Open Access Journals (Sweden)

    Carla Bresciani

    2014-02-01

    Full Text Available The aims of the study were to identify microbial flora in boar semen under field conditions in northern Italy, to investigate antibiotic resistance and sensitivity of isolated bacteria, and to evaluate elimination of bacteria after storage in two types of extenders added with different antibiotics (amikacin vs gentamicin. A total of 60 boars were collected in 13 pig farms. Bacteriological and mycological investigations were performed immediately on raw semen samples, then at 48 and 120 h of storage on semen diluted randomly in a new short-term modified extender (ME-S or in a commercial one (CRONOSTM. Bacterial contamination was found in 63% of raw semen samples and different bacterial species were isolated: E.coli, Serratia marcescens, Staphylococcus epidermidis and aureus, Proteus spp., Streptococcus spp. and Pseudomonas aeruginosa. E. coli was the most isolated contaminant (53%; Pseudomonas aeruginosa was found only in one semen sample. The analysis of variance of factors affecting contamination levels was significant for the farm of origin (P<0.05 and not significant for the breed. Antibiotic resistance of these bacteria was assessed using different antibiotics. Significant differences (P<0.05 between observed and expected frequencies of bacterial isolates resistant or not to the antibiotics contained in the extenders were found. At 48 h of storage a reduction of aerobic contamination was found after ME-S dilution by 85.3% and after CRONOSTM by 63.8%. This paper proved the presence of pathogenic bacteria in semen. We thus believe it is highly advisable to perform periodic microbiological screening of boar semen in the swine industry to avoid the use of low sperm quality.

  15. Effect of varicocelectomy on testis volume and semen parameters in adolescents: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Tie Zhou

    2015-01-01

    Full Text Available Varicocele repair in adolescent remains controversial. Our aim is to identify and combine clinical trials results published thus far to ascertain the efficacy of varicocelectomy in improving testis volume and semen parameters compared with nontreatment control. A literature search was performed using Medline, Embase and Web of Science, which included results obtained from meta-analysis, randomized and nonrandomized controlled studies. The study population was adolescents with clinically palpable varicocele with or without the testicular asymmetry or abnormal semen parameters. Cases were allocated to treatment and observation groups, and testis volume or semen parameters were adopted as outcome measures. As a result, seven randomized controlled trials (RCTs and nonrandomized controlled trials studying bilateral testis volume or semen parameters in both treatment and observation groups were identified. Using a random effect model, mean difference of testis volume between the treatment group and the observation group was 2.9 ml (95% confidence interval [CI]: 0.6, 5.2; P< 0.05 for the varicocele side and 1.5 ml (95% CI: 0.3, 2.7; P< 0.05 for the healthy side. The random effect model analysis demonstrated that the mean difference of semen concentration, total semen motility, and normal morphology between the two groups was 13.7 × 10 6 ml−1 (95% CI: −1.4, 28.8; P = 0.075, 2.5% (95% CI: −3.6, 8.6; P= 0.424, and 2.9% (95% CI: −3.0, 8.7; P= 0.336 respectively. In conclusion, although varicocelectomy significantly improved bilateral testis volume in adolescents with varicocele compared with observation cases, semen parameters did not have any statistically significant difference between two groups. Well-planned, properly conducted RCTs are needed in order to confirm the above-mentioned conclusion further and to explore whether varicocele repair in adolescents could improve subsequently spontaneous pregnancy rates.

  16. Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

    Directory of Open Access Journals (Sweden)

    Joanna Jurewicz

    2013-10-01

    Full Text Available Objectives: Growing evidence supports the reproductive and developmental toxicity of polycyclic aromatic hydrocarbons (PAHs from prenatal and postnatal exposure, but the results of epidemiological studies regarding harmful effects of PAHs exposure on male reproductive system still remain limited and inconclusive. The aim of the present study was to investigate the relationship between 1-hydroxypyrene, a biomarker of polycyclic aromatic hydrocarbons exposure and semen quality. Materials and Methods: The study population consisted of 277 men attending an infertility clinic for diagnostic purposes and having normal semen concentration of 20-300 mln/ml or slight oligozoospermia (semen concentration: 15-20 mln/ml (WHO 1999. All the men were healthy and under 45 years of age. All participants were interviewed and provided a semen sample. The interview included questions concerning demographics, socio-economic status, medical history related to past diseases which may have an impact on semen quality, lifestyle factors and occupational information. Concentrations of 1-hydroxypyrene (1-OHP in the urine samples were analyzed using high performance liquid chromatography (HPLC. Results: A positive association was found between the level of 1-OHP in urine and sperm neck abnormalities as well as the percentage of static sperm cells (p = 0.001, p = 0.018, respectively. Additionally, exposure to PAHs measured by 1-OHP in urine decreased semen volume and the percentage of motile sperm cells (p = 0.014, p = 0.0001, respectively. Conclusions: Presented findings indicate that the environmental level of PAHs exposure adversely affects male semen quality. The future large-scale studies should incorporate different biomarkers to generate a more accurate and full assessment of the effects of PAHs exposure on male fertility.

  17. Use of computer-assisted semen analysis for evaluation of Rosy-faced lovebird (Agapornis roseicollis) semen collected in different periods of the year.

    Science.gov (United States)

    Dogliero, Andrea

    2015-01-01

    The seminal characteristics of the Rosy-faced lovebird (Agapornis roseicollis) were analyzed, both in and out of season, using a computer-aided sperm analyzer. Avian semen collection and artificial insemination techniques have great potential for captive breeding programs, and computer-aided sperm analyzer allows an objective and quantitative assessment of sperm motility and kinetics. Although Agapornis roseicollis is a largely diffuse species, its seminal parameters have never been fully investigated. Using the massage technique, 38 ejaculates were collected in the breeding season, and 6 ejaculates were collected outside of the breeding season. Semen color, volume, degree of contamination, spermatozoa concentration, total and progressive motility, and kinetics parameters were recorded. Seasonal significant differences were found in the ejaculate volume (1.6 0.6 and 1.1 0.2 mL in and out season, respectively, P < 0.01) and spermatozoa concentration (7194.0 6735.1 x 10(6) and 327.5 314.0 x 10(6) spermatozoa/mL, P < 0.01); among the motility parameters, only beat cross frequency, indicating the frequency of flagellar beats, was significantly higher out of the reproductive season (29.8 2.6 vs. 24.5 3.8 Hz, P < 0.01). There was very large individual variation in semen characteristics that could qualify a male as a potentially good or bad semen donor for future assisted reproduction in captivity. PMID:25441497

  18. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study.

    Science.gov (United States)

    Gonzalez-Pena, Dianelys; Knox, Robert V; Rodriguez-Zas, Sandra L

    2016-01-15

    The economic impact of selection for semen traits on pig production systems and potential interaction with artificial insemination (AI) technique and semen dose remains partially understood. The objectives of this study were to compare the financial indicators (gross return, net profit, cost) in a three-tier pig production system under one of two selection strategies: a traditional strategy including nine paternal and maternal traits (S9) and an advanced strategy that adds four semen traits (S13). Maternal traits included the number of pigs born alive, litter birth weight, adjusted 21-day litter weight, and the number of pigs at 21 days, and paternal traits included days to 113.5 kg, back fat, average daily gain, feed efficiency, and carcass lean percentage. The four semen traits included volume, concentration, progressive motility of spermatozoa, and abnormal spermatozoa. Simultaneously, the impact of two AI techniques and a range of fresh refrigerated semen doses including cervical AI with 3 × 10(9) (CAI3) and 2 × 10(9) (CAI2) sperm cells/dose, and intrauterine AI with 1.5 × 10(9) (IUI1.5), 0.75 × 10(9) (IUI0.75), and 0.5 × 10(9) (IUI0.5) sperm cells/dose were evaluated. These factors were also evaluated using a range of farrowing rates (60%-90%), litter sizes (8-14 live-born pigs), and a selected semen collection frequency. The financial impact of the factors was assessed through simulation of a three-way crossbreeding system (maternal nucleus lines A and B and paternal nucleus line C) using ZPLAN. The highest return on investment (profit/cost) of boars was observed at 2.33 collections/wk (three periods of 24 hours between collections). Under this schedule, a significant (P profit. The highest gross return was obtained under S13 with IUI0.75 and IUI0.5. The net profit of S13 was 34.37% higher than the traditional S9 (P profit favored IUI0.5 with relative differences of 4.13%, 2.41%, 1.72%, and 0.43% compared to CAI3, CAI2, IUI1.5, and IUI0.75, respectively. The advanced selection strategy proposed including four semen traits is recommended on the basis of the higher profitability relative to the traditional strategy. PMID:26435262

  19. Sperm head phenotype and male fertility in ram semen.

    Science.gov (United States)

    Maroto-Morales, A; Ramón, M; García-Álvarez, O; Montoro, V; Soler, A J; Fernández-Santos, M R; Roldan, E R S; Pérez-Guzmán, M D; Garde, J J

    2015-12-01

    Although there is ample evidence for the effects of sperm head shape on sperm function, its impact on fertility has not been explored in detail at the intraspecific level in mammals. Here, we assess the relationship between sperm head shape and male fertility in a large-scale study in Manchega sheep (Ovis aries), which have not undergone any selection for fertility. Semen was collected from 83 mature rams, and before insemination, head shapes were measured for five parameters: area, perimeter, length, width, and p2a (perimeter(2)/2×π×area) using a computer-assisted sperm morphometric analysis. In addition, a cluster analysis using sperm head length and p2a factor was performed to determine sperm subpopulations (SPs) structure. Our results show the existence of four sperm SPs, which present different sperm head phenotype: SP1 (large and round), SP2 (short and elongated), SP3 (shortest and round), and SP4 (large and the most elongated). No relationships were found between males' fertility rates and average values of sperm head dimensions. However, differences in fertility rates between rams were strongly associated to the proportion of spermatozoa in an ejaculate SP with short and elongated heads (P < 0.001). These findings show how the heterogeneity in sperm head shape of the ejaculate has an effect on reproductive success, and highlight the important role of modulation of the ejaculate at the intraspecific level. PMID:26318229

  20. Semen cryopreservation in fish: effects on sperm motility and fertility

    International Nuclear Information System (INIS)

    The cryopreservation of semen in fish, as in many species even shows effects that decrease sperm quality and directly engage cell ability to successfully participate in the processes of fertilization and embryonic development. the characteristics such as mobility and fertilizing capacity of fertilization of sperm are considered to be quality criteria that allow to measure the success or failure of the process, since they are considered integrative variables, being indicators that depend not on a single factor, but on the stability and welfare of all structures, enzymes and subcellular functional compounds that give place to these spermatic characteristics. membrane damage (Adenylate cyclase, ion channels, grouping of other proteins, among others) and their implication in the route of signaling pathway leading to spermatic activation, ATP degradation and fragmentation of nuclear and mitochondrial DNA (genome), degradation of kinase enzymes and other cytosolic proteins (proteome) are considered today, as some of the molecular factors that most affect during cryopreservation and markedly decreasing the fertilizing capacity and mobility of sperm in fish. Proposals on the molecular mechanisms, by which these subcellular factors interact and act as consequence of cryopreservation, are some of the topics covered in this review. Understanding the principles and factors that are involved in the origin of such damages, will allow to improved cryopreservation processes, making them less harmful and more efficient.

  1. Study of correlation between reaction time and refractory period (as indices of libido) with semen characteristics in ArkharmerinoMoghani and BaluchiMoghani rams

    OpenAIRE

    Rahimi, Ali A. R.; Gholamali Moghaddam; Mohamad M. Pourseif

    2012-01-01

    This study was conducted on 10 crossbred rams consisted of 5 ArkharMerinoMoghani (AMMG) and 5 BaluchiMoghani (BLMG), to analyze correlation between semen characteristics and libido activity. The semen samples were evaluated for semen volume, total sperm per ejaculate (TSE), spermatozoa concentration (SC), color, wave motion, spermatozoa progressive motility, percentage of live and abnormal spermatozoa, pH, rate of metabolic activity of spermatozoa and semen index. The libido of rams was m...

  2. Evaluation of Semen Fertility of Bulls by Non-return Rate at 60 Days of Cows under Artificial Insemination Programme in Bangladesh

    OpenAIRE

    M.J.U. Sarder

    2006-01-01

    The present study were to evaluate the effect of individual bull, semen types, quality of bull semen, sources of semen on Non-return Rate (NRR) at 60 days of cows under field condition. A total 75550 cows were inseminated with 71 bull semens from Central Cattle Breeding Station and Dairy Farm (CCBSDF), Savar, Dhaka, Rajshahi Dairy and Cattle Improvement Farm (RDCIF), Rajabarihat and District Artificial Insemination Centre (DAIC), Rajshahi under 40 Artificial Insemination (AI) sub-centres/poin...

  3. Relationship of spermatozoal DNA fragmentation with semen quality in varicocele-positive men.

    Science.gov (United States)

    Moazzam, A; Sharma, R; Agarwal, A

    2015-10-01

    The aim of the study was to assess the semen quality and levels of spermatozoal nuclear DNA fragmentation in subfertile subjects clinically diagnosed with varicocele, subfertile subjects without varicocele and healthy fertile controls. Semen samples were obtained from 302 subjects. Of them, 115 were healthy fertile controls having normal semen characteristics, 121 subfertile men diagnosed with varicocele, both, clinically and on ultrasonography, while 66 subjects were subfertile with no varicocele. Spermatozoal concentration, percentage motility, morphology and DNA fragmentation were measured. In the study population, deterioration in semen quality-decreased spermatozoal concentration, percentage motility and normal morphology was seen in subfertile subjects, especially with varicocele. Highest spermatozoal DNA fragmentation was observed in varicocele-positive subjects as compared with varicocele-negative subjects and healthy fertile controls. Significant negative correlation was seen between spermatozoal DNA fragmentation and concentration (r=-0.310), motility (r=-0.328) normal morphology, WHO method (r=-0.221) and Tygerberg strict criteria (r=-0.180) in the varicocele-positive subfertile subjects. In conclusion, this study suggests existence of a negative relationship between spermatozoal DNA fragmentation and semen quality in varicocele-positive subfertile subjects. PMID:25346327

  4. The combined use of embryos and semen for cryogenic conservation of mammalian livestock genetic resources.

    Science.gov (United States)

    Boettcher, Paul J; Stella, Alessandra; Pizzi, Flavia; Gandini, Gustavo

    2005-01-01

    The objective of this empirical simulation study was to evaluate the use of a combination of semen and embryos in the creation of gene banks for reconstruction of an extinct breed. Such an approach was compared for banks with varying proportions of embryos on the basis of the amount of the material to be stored, time for reconstruction, maintenance of genetic variability, and probability of failure during reconstruction. Four types of populations were simulated, based on reproductive rate: single offspring, twinning, enhanced reproduction, and litter bearing. Reconstruction was simulated for banks consisting of different combinations of semen and reduced numbers of embryos (expressed as a percentage of the material needed for a bank containing exclusively embryos and ranging from 10 to 90%). The use of a combination of semen and embryos increased the number of insemination cycles needed for reconstruction and the level of genetic relatedness in the reconstructed population. The risk for extinction was unacceptably high when a very low proportion of embryos (<20%) was used. However, combining semen with embryos could decrease costs, allowing for the conservation of more breeds, and specific strategies for semen use could decrease the level of relationships in the reconstructed breed. PMID:16277973

  5. Effect of goserelin and leuprolide added to the semen on reproductive performance in rabbits - Short communication.

    Science.gov (United States)

    Gogol, Piotr

    2016-03-01

    The aim of this study was to evaluate the ability of two synthetic GnRH analogues, goserelin and leuprolide, to induce ovulation in rabbit does using intravaginal administration. A total of 252 primiparous lactating does were randomly divided into five groups that, at the time of insemination, received the following treatments for ovulation induction: 1 g of buserelin administered intramuscularly (control group), 5 g of goserelin added to the semen dose (Group G5), 10 g of goserelin added to the semen dose (Group G10), 5 g of leuprolide added to the semen dose (Group L5), and 10 g of leuprolide added to the semen dose (Group L10). The kindling rate was 80.5% in Group G10 and 75.0% in Group L10; these values are comparable to the kindling rate obtained in the control group (85.9%). The kindling rates in Groups G5 and L5 were significantly lower than in the control group (60.0%, 54.2% and 85.9%, respectively). The number of live-born rabbits was not significantly affected by the ovulation induction treatment. As regards the total number of rabbits born the only significant difference was observed between Groups G5 and L5. This study shows the possibility of inducing ovulation in rabbits by adding goserelin and leuprolide directly to the semen dose. PMID:26919148

  6. Motilidad y fertilidad del semen de carnero descongelado a dos diferentes ritmos de temperatura

    Directory of Open Access Journals (Sweden)

    Rosa Bertha Angulo Mejorada

    1999-01-01

    Full Text Available El objetivo del presente trabajo fue comparar el efecto de dos temperaturas y tiempos de descongelacin sobre la motilidad y fertilidad del semen del ovino. Se utiliz un total de 208 ovejas y 13 carneros de diferentes razas. El semen se obtuvo por medio de una vagina artificial. Se evalu, se diluy con Tris -cido ctrico-fructosa-glicerol-yema de huevo y se centrifug a 200 g/15 min; el paquete celular se resuspendi con el mismo diluyente. El semen diluido se congel en pajillas francesas de 0.25 ml con 300 millones de espermatozoides mtiles. Se determin la motilidad al descongelamiento en 212 pajillas descongeladas en bao Mara a 36C/8 seg (grupo 1 o a 70C/4 seg (grupo 2 y otras 208 dosis se utilizaron en la inseminacin artificial de las ovejas por va intracervical. Se determin el porcentaje de ovejas paridas/ovejas inseminadas con semen descongelado a diferentes ritmos. La motilidad al descongelar fue de 51.28% y 47.98% en el semen descongelado de los grupos 1 y 2, respectivamente, y la fertilidad de 30.7% y 29.2%, para los mismos grupos, respectivamente, sin que existiera diferencia significativa entre grupos (C2; P>0.05. Se concluye que la descongelacin de las pajillas a 36C durante 8 segundos es un mtodo ms prctico e inocuo.

  7. EFFECT OF ANTIBIOTICS IN EXTENDER ON FERTILITY OF LIQUID BUFFALO BULL SEMEN

    Directory of Open Access Journals (Sweden)

    S. AKHTER, M. SAJJAD, S. M. H. ANDRABI1, N. ULLAH1 AND M. QAYYUM

    2007-01-01

    Full Text Available This study was carried out to determine if a new antibiotic combination comprising of gentamycin, tylosin and linco-spectin (GTLS in extender is suitable for improvement in fertility of liquid buffalo bull semen through artificial insemination (AI. Two consecutive ejaculates per week (4 weeks were collected from three Nili-Ravi buffalo bulls of known fertility by using artificial vagina. The pooled ejaculates were split-sampled and diluted with skimmed milk extender (37oC; 10x106 motile spermatozoa/ml containing either SP (streptomycin 1000 μg/ml and penicillin 1000 iu/ml or GTLS (gentamycin 500 μg/ml, tylosin 100 μg/ml, lincomycin 300 μg/ml, and spectinomycin 600 μg/ml. Liquid semen was stored at 5°C for seven days. Fertility, based on 90-days first service pregnancy rate, was determined under field conditions. The fertility rates for SP-based vs. GTLS-containing liquid semen of buffalo bull were 58.55 and 60.00%, respectively, the difference was non significant. The fertility rates also did not differ (P>0.05 due to antibiotics at different days of storage of liquid semen at 5oC. In conclusion, GTLS, in skimmed milk extender compared to SP, did not significantly improve the fertility of chilled buffalo bull semen.

  8. Effect of varicocelectomy on testis volume and semen parameters in adolescents: a meta-analysis.

    Science.gov (United States)

    Zhou, Tie; Zhang, Wei; Chen, Qi; Li, Lei; Cao, Huan; Xu, Chuan-Liang; Chen, Guang-Hua; Sun, Ying-Hao

    2015-01-01

    Varicocele repair in adolescent remains controversial. Our aim is to identify and combine clinical trials results published thus far to ascertain the efficacy of varicocelectomy in improving testis volume and semen parameters compared with nontreatment control. A literature search was performed using Medline, Embase and Web of Science, which included results obtained from meta-analysis, randomized and nonrandomized controlled studies. The study population was adolescents with clinically palpable varicocele with or without the testicular asymmetry or abnormal semen parameters. Cases were allocated to treatment and observation groups, and testis volume or semen parameters were adopted as outcome measures. As a result, seven randomized controlled trials (RCTs) and nonrandomized controlled trials studying bilateral testis volume or semen parameters in both treatment and observation groups were identified. Using a random effect model, mean difference of testis volume between the treatment group and the observation group was 2.9 ml (95% confidence interval [CI]: 0.6, 5.2; P3.0, 8.7; P= 0.336) respectively. In conclusion, although varicocelectomy significantly improved bilateral testis volume in adolescents with varicocele compared with observation cases, semen parameters did not have any statistically significant difference between two groups. Well-planned, properly conducted RCTs are needed in order to confirm the above-mentioned conclusion further and to explore whether varicocele repair in adolescents could improve subsequently spontaneous pregnancy rates. PMID:25677136

  9. Does weight loss improve semen quality and reproductive hormones? results from a cohort of severely obese men

    DEFF Research Database (Denmark)

    Hkonsen, Linn Berger; Thulstrup, Ane Marie; Aggerholm, Anette Skrbech; Olsen, Jrn; Bonde, Jens Peter; Andersen, Claus Yding; Bungum, Mona; Ernst, EH; Hansen, Mette Lausten; Ernst, Erik Hagen; Ramlau-Hansen, Cecilia Hst

    2011-01-01

    A high body mass index (BMI) has been associated with reduced semen quality and male subfecundity, but no studies following obese men losing weight have yet been published. We examined semen quality and reproductive hormones among morbidly obese men and studied if weight loss improved the reprodu...

  10. Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners

    DEFF Research Database (Denmark)

    Bonde, J P; Ernst, Erik; Jensen, T K; Hjøllund, Niels Henrik Ingvar; Kolstad, Henrik; Henriksen, T B; Scheike, Thomas; Giwercman, A; Olsen, J; Skakkebaek, N E

    1998-01-01

    Semen analysis is part of the routine assessment of infertile couples. WHO defines a sperm concentration above 20x10(6) per mL seminal fluid as normal. We studied the association between semen quality and the probability of conception in a single menstrual cycle in Danish couples with no previous...

  11. Cryopreservation of canine semen after cold storage in a Neopor box: effect of extender, centrifugation and storage time.

    Science.gov (United States)

    Hidalgo, M; Portero, J M; Demyda-Peyrás, S; Ortiz, I; Dorado, J

    2014-07-01

    The aim of this work was to assess the combined effect of sperm centrifugation, semen extender and storage time before freezing on post-thaw sperm quality and freezability on chilled stored canine semen in a Neopor box. Sperm parameters evaluated were total and progressive sperm motility by Computer-Assisted Sperm Analysis (CASA) and sperm viability and acrosome integrity using a triple fluorescent stain. Sperm quality and freezability indexes were also studied. First, the effect of centrifugation and two commercial extenders from Minitübe (Biladyl A and CaniPRO Freeze A) was evaluated in chilled semen after 24 and 45 hours of cold storage. No significant differences were observed between treatments in almost all the sperm parameters assessed. Secondly, chilled semen was frozen after 24 and 45 hours of cold storage in a Neopor box. The best results were obtained when semen was centrifuged, chilled with CaniPRO Freeze A and then frozen after 24 hours of cold storage, showing no differences in both post-thaw sperm quality and freezability in comparison with semen immediately frozen after collection. In conclusion, dog semen centrifuged after collection and extended with CaniPRO Freeze can be frozen after 24 hours of cold storage in a Neopor box, obtaining similar results to semen immediately frozen after collection. PMID:24799391

  12. Caractersticas Bioqumicas del Plasma Seminal Fresco y Congelado/Descongelado de Alpaca (Vicugna pacos) / Biochemical characteristics of fresh and freeze/thawed seminal plasma of alpaca (Vicugna pacos)

    Scientific Electronic Library Online (English)

    Hugo, Daz V; Juan, Espinoza B; Wilfredo, Huanca L; Bernardo, Lopez-Torres; Jos, Rodrguez G.

    2015-01-01

    Full Text Available El objetivo del estudio fue determinar y comparar las caractersticas bioqumicas del plasma seminal de alpacas en fresco y descongelado. Se recolect semen, mediante electroeyaculacin, de cuatro alpacas adultas, una vez por semana por cuatro semanas. El semen se centrifug y el plasma seminal fue [...] separado. Una parte se analiz en fresco y la otra parte se almacen en nitrgeno lquido por un mes. Se le hizo el anlisis bioqumico a ambos juegos de muestras. Se determinaron los niveles de glucosa, colesterol total, colesterol-HDL, triglicridos, protenas totales, albmina, calcio, fosfatasa alcalina, ALT y ?-GT. Solo los valores de triglicridos descendieron significativamente por el proceso de congelacin/descongelacin (p Abstract in english The aim of this study was to determine and compare the biochemical characteristics of fresh and thawed seminal plasma of alpacas. Semen was collected by electroejaculation from four adult males, once a week per four weeks. Semen was centrifuged and the seminal plasma was separated. One part was anal [...] ysed fresh and other stored for one month on liquid nitrogen and then thawed and analysed. Levels of glucose, total cholesterol, HDL-cholesterol, triglycerides, total protein, albumin, calcium, alkaline phosphatase, ALT and ?-GT were determined. Only the triglycerides significantly decreased due to the process of freeze/thawed, where the values were 44.12 7.38 and 27.31 4.65 mg/dl in fresh and thawed respectively.

  13. Semen quality and reproductive hormone levels in men from Southern Spain

    DEFF Research Database (Denmark)

    Fernandez, M F; Duran, I; Olea, N; Avivar, C; Vierula, M; Toppari, J; Skakkebaek, N E; Jørgensen, N

    2012-01-01

    have a higher risk of testicular cancer. According to the testicular dysgenesis syndrome (TDS) concept that suggests a link between risk of impaired semen quality and increased risk of testicular cancer, Spanish men would be expected to have a semen quality at a normal level because of their very low...... investigations took place from November 2001 to December 2002. Adjusting for effects of confounders, the median sperm concentration and total sperm count were 62 (95% confidence interval 47-82) million/mL and 206 (153-278) million, respectively. The median numbers of motile and morphologically normal spermatozoa...... men the Southern Spain, the results show that these have normal semen quality and reproductive hormone levels as expected in a population with a low incidence of testicular cancer....

  14. Studies on the collection and storage of semen from the African Elephant, loxodonta african

    Directory of Open Access Journals (Sweden)

    R.C. Jones

    1975-07-01

    Full Text Available Methods of semen collection following electrical stimulation are described. Semen was also collected from the male reproductive tract of slaughtered animals for several studies. Spermatozoa flushed from the male reproductive tract were immotile, but, dilution with buffered saline was sufficient to induce motility. It was found that the spermatozoa were sensitive to hypotpnic solutions and rapid cooling to 5C. Spermatozoa were frozen in egg yolk-citrate diluents. Dimethyl sulphoxide (DMSO was a better protective agent than glycerol. Eight per cent v/v was the best concentration of DMSO when used alone, but better results were obtained using a combination of 7% v/v DMSO and 1% v/v glycerol. A semen bank was established using these concentrations of protective agents and thawed samples from the bank showed good viability in the laboratory.

  15. Use of combinations of invitro quality assessments to predict fertility of bovine semen.

    Science.gov (United States)

    Sellem, E; Broekhuijse, M L W J; Chevrier, L; Camugli, S; Schmitt, E; Schibler, L; Koenen, E P C

    2015-12-01

    Predicting invivo fertility of bull ejaculates using invitro-assessed semen quality criteria remains challenging for the breeding industry. New technologies such as computer-assisted semen analysis (CASA) and flow cytometry may provide accurate and objective methods to improve semen quality control. The aim of this study was to evaluate the relationship between semen quality parameters and field fertility of bull ejaculates. A total of 153 ejaculates from 19 Holstein bulls have been analyzed using CASA (postthawing semen motility and morphology) and several flow cytometric tests, including sperm DNA integrity, viability (estimated by membrane integrity), acrosomal integrity, mitochondria aerobic functionality and oxidation. Samples were analyzed both immediately after thawing and after 4hours at 37C. A fertility value (FV), based on nonreturn rate at 56 days after insemination and adjusted for environment factors, was calculated for each ejaculate. Simple and multiple regressions have been used to correlate FV with CASA and flow cytometric parameters. Significant simple correlations have been observed between some parameters and FV (e.g., straight line velocity [?m/s], r(2)=-0.12; polarized mitochondria sperm (%), r(2)=0.07), but the relation between simple parameter and FV was too week to predict the fertility. Partial least square procedure identified several mathematical models combining flow cytometer and CASA variables and had better correlations with FV (adjusted r(2) ranging between 0.24 and 0.40 [P<0.0001], depending on the number of included variables). In conclusion, this study suggests that quality assessment of thawed bull sperm using CASA and flow cytometry may provide a reasonable prediction of bovine semen fertility. Additional work will be required to increase the prediction reliability and promote this technology in routine artificial insemination laboratory practice. PMID:26296523

  16. The effect of Curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen.

    Science.gov (United States)

    Chanapiwat, Panida; Kaeoket, Kampon

    2015-09-01

    The aim of this study was to determine the optimal concentration of curcumin needed for cryopreservation of boar semen. Semen samples (n?=?9) were collected from nine Duroc boars which having proven fertility were used for routine artificial insemination. Semen samples were collected and divided into six groups (groups A-F) according to various concentrations of curcumin in freezing extender (i.e. 0, 0.125, 0.25, 0.50, 0.75 and 1.0?mmol/L, respectively). The semen was frozen by traditional liquid nitrogen vapor method and stored at -196C in the liquid nitrogen tank. After storage, frozen semen samples were thawed at 50C for 12?s and evaluated for progressive motility, viability and acrosome integrity. The present results indicated that the addition of curcumin at 0.25 (group C) or 0.50?mmol/L curcumin (group D) yielded the higher percentage of progressive motility (33.3 and 36.1%, respectively) (P?semen. PMID:26032188

  17. Influence of semen collection method on sperm cryoresistance in small ruminants.

    Science.gov (United States)

    Jiménez-Rabadán, P; Soler, A J; Ramón, M; García-Álvarez, O; Maroto-Morales, A; Iniesta-Cuerda, M; Fernández-Santos, M R; Montoro, V; Pérez-Guzmán, M D; Garde, J J

    2016-04-01

    Semen collection for cryopreservation is a key step for small ruminant conservation programs. While in these species semen is mainly collected via artificial vagina (AV), electroejaculation (EE) provides a viable alternative for untrained males. Herein we investigated the effect of semen collection method on post-thaw sperm quality by comparing two small ruminant species, sheep and goats. Semen from Blanca-Celtibérica bucks and Manchega rams was collected by AV and EE on the same day and cryopreserved using a standard protocol. At thawing, sperm motion parameters were evaluated by CASA, whereas membrane stability (YO-PRO-1), sperm viability (propidium iodide, PI) and mitochondrial activity (Mitotracker Deep Red) were analyzed using flow cytometry. The semen collection method negatively affected post-thaw sperm quality in bucks but not in rams. Thus, in bucks, post-thaw sperm motility was higher for samples collected by AV as compared to those obtained via EE. Similarly, post-thaw sperm parameters evaluated by flow cytometry were worse for buck samples collected by EE than those collected by AV in the same species, or than ram samples regardless of collection method. These results suggest that ovine and caprine spermatozoa have a different response to the cryopreservation process depending upon the semen collection method used. We hypothesize that the EE procedure may lead to changes in the composition of the ejaculate in bucks that would make spermatozoa more susceptible to the cryopreservation process, whereas this procedure would have had no effect on ram spermatozoa. This assumption requires further investigation. PMID:26917359

  18. SEMEN PRODUCTION AND PRODUCTIVE LIFE OF SAHIWAL BULLS: RELATIONSHIP WITH GENETIC WORTH

    Directory of Open Access Journals (Sweden)

    M. S. KHAN, A. A. BHATTI, S. A. BHATTI1 AND M. ASHIQ2

    2007-01-01

    Full Text Available Objective of the present study was to document the semen producing ability, productive life and genetic ability for lactation milk yield of Sahiwal bulls used for artificial insemination (AI in Punjab and to find the impact of AI bulls on the improvement of Sahiwal cattle. Data from Semen Production Unit (SPU, Qadirabad, Sahiwal, Pakistan were used for this purpose. A repeatability animal model was used for estimation of breeding values for lactation milk yield. Productive life of a bull was calculated as a difference between culling age and the age at first ejaculation. Number of bulls brought to SPU varied from 9 to 102 for any year. Average number of doses of semen produced by any bull for a year varied from 724 to 5745. On the average, 238 bulls produced 17143 ± 1164 semen doses during their average stay of 5.4 ± 0.2 years. About 50% of the bulls stayed for less than four years at the SPU; with a maximum range of 14 years. Progeny tested bulls (n=90 produced 5000 and 10000 semen doses (Y in three and four years of stay (X, respectively (Y = 24.8 + 2.3635 X - 0.0112 X2. To produce 20,000 doses, it is predicted that bulls need to stay for six and a half years at the SPU. There was no association between breeding values for lactation milk yield estimated under a repeatability animal model (EBVs and number of semen doses produced (r = 0.17 and EBVs and number of daughters. Lack of genetic superiority of bulls used indicated that AI did not bring desired genetic improvement in Sahiwal cattle in the present situation. Modifications for judicious utilization of bulls are suggested along with improvements in data recording.

  19. Decline of semen quality and increase of leukocytes with cigarette smoking in infertile men

    Directory of Open Access Journals (Sweden)

    Zhi Hong Zhang

    2013-01-01

    Full Text Available Background: Previous researches about the effect of smoking on semen quality are contradictory, and the mechanism behind the harmful effect of smoking on semen quality still remains unclear until today. Objective: The objectives of this study are evaluation of the relationship between smoking and fertility, investigation of the effects of cigarette smoking on sperm parameters and detection of presence of leukocytes within the semen of idiopathic infertile men from Northeastern China. Materials and Methods: A retrospective study of 1512 infertile patients who visited affiliated hospitals of Jilin University from 2007-2010 were enrolled in this study. Patients were assigned into one non-smoking and one smoking group which was divided into mild, moderate and heavy subgroups. Sperm parameters (including leukocytes and sperm morphology analysis were performed using standard techniques. Results: Compared with non-smokers, smokers had a significant decrease in semen volumes (p=0.006, rapid progressive motility (p=0.002 and sperm viability (p=0.019; moreover, smokers had a significant increase in the levels of immotile sperms (p=0.005 and semen leukocytes (p=0.002; pH and sperm concentration were not statistically significant (p=0.789 and p=0.297 respectively. Sperm motion parameters were all lower in the smokers except for beat-cross frequency (Hz (BCF. Further, the percentage of normal morphology sperm was decreased significantly in smokers (p=0.003, the sperm morphology was worse with increasing degree of smoking. Conclusion: These findings suggest that smoking leads to a significant decline in semen quality and higher levels of leukocytes, thus smoking may affects the fertilization efficiency.

  20. Computer aided boar semen motility analysis for cereulide detection in different food matrices.

    Science.gov (United States)

    Rajkovic, Andreja; Uyttendaele, Mieke; Debevere, Johan

    2007-02-28

    Computer Aided Semen Analysis (CASA) study of the boar semen motility has been demonstrated to be an appropriate assay for detection of cereulide (Bacillus cereus emetic toxin). Application of the boar semen bio-assay to detect cereulide directly in foods requires investigation of potential interference of food components, preservatives and other microbial and chemical food contaminants with the bio-assay. Current study provides evidence that none of included Staphylococcus aureus enterotoxins A, B, C and D nor B. cereus Hemolysin BL (HBL) and non-hemolytic enterotoxin (NHE) and three mycotoxins (Sterigmatocystin, Fumonisin B1 and Patulin) exhibited a toxic impact on semen progressive motility. Aflatoxin M1, M3 and zearalenone impaired semen motility only at concentrations (0.004 mg ml(-1), 0.1 mg ml(-1) and 10 mg ml(-1), respectively) much higher than those found in foods and those permitted by legislation, in comparison to cereulide which induces motility cease at concentrations lower than 20 ng ml(-1). Ten commonly used preservatives, namely potassium sorbate, sodium benzoate, (DL) malic acid, citric acid, (L+) tartaric acid, acetic acid, (DL) lactic acid, (L+) ascorbic acid, sodium chloride and sucrose induced no cease in spermatozoa motility even at preservative concentrations higher than permitted by legislation. Dioxins, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and acrylamide had no acute effect on spermatozoa motility at concentrations of 500 and 10,000 mg ml(-1), respectively. Robustness of computer aided boar semen motility analysis, tested with 14 different foods inoculated with cereulide producing B. cereus, showed distinct cereulide production in seven samples (although B. cereus growth to counts higher than 8 log CFU g(-1) was noted in 11 samples), in amounts close to those reported in foodborne outbreaks. Test evaluation in 33 samples suspected to hold cereulide showed actual cereulide presence in ten samples and no interference of food matrix with the assay. PMID:17174428

  1. Genome-wide association study for semen production traits in Holstein-Friesian bulls.

    Science.gov (United States)

    Suchocki, T; Szyda, J

    2015-08-01

    Identifying genomic regions, particularly individual genes associated with semen quality traits, may be very important for improving sire fertility via selective breeding. The aim of the study was to estimate (co)variance components and effects of single nucleotide polymorphisms (SNP) from the Illumina BovineSNP50 BeadChip (Illumina, San Diego, CA) on semen production traits and to find candidate genes for these traits. The analyzed data set originates from the Polish Holstein-Friesian dairy cattle population and consists of 1,212 bulls kept at 4 artificial insemination stations. For each bull, 5 semen production traits were collected: sperm concentration, semen volume, number of spermatozoa, motility, and motility score. A multitrait mixed model was used to estimate genetic parameters. The parameters obtained were used to estimate SNP effects for each trait separately by the mixed model, which is used in the Polish direct genomic value project. Additionally, genes located in the vicinity of significant SNP were selected as candidate genes. For motility, 20 genome-wide significant SNP, located on 12 autosomes, were identified. For sperm concentration, we found 7 significant SNP: 3 on chromosome X, and 1 on chromosomes 1, 6, 23, and 24. For semen volume and motility score, 3 and 1 significant SNP were detected, respectively. All these SNP were located on chromosome X. For the number of spermatozoa, 12 significant SNP were observed. Six SNP were located on chromosome X, 3 on chromosome 8, and 1 on chromosomes 2, 7, and 16. This study clearly indicated a key role of the X chromosome in the determination of semen quality and emphasized that including such traits into genetic evaluation should be strongly considered. PMID:26051317

  2. EVALUACIN DE LA MOVILIDAD DEL SEMEN CRIOPRESERVADO DE CABALLOS CRIOLLO COLOMBIANO POR UN SISTEMA ANALIZADOR DE CLASE

    Directory of Open Access Journals (Sweden)

    Giovanni Restrepo

    2013-12-01

    Full Text Available La reproduccin juega un papel fundamental en el mejoramiento gentico de los equinos. La criopreservacin del semen equino es una tcnica complementaria a los procesos de biotecnologa reproductiva; sin embargo, solamente el 40% de los caballos producen semen que se puede criopreservar, con una alta variabilidad en su fertilidad. La confiabilidad de los mtodos de evaluacin del semen es trascendente para determinar su fertilidad potencial; no obstante, el anlisis convencional por evaluacin microscpica ha sido poco efectivo, en este sentido. El anlisis de semen asistido por computador (CASA permite una medicin objetiva de la movilidad de semen. Esta investigacin tuvo como objetivo comparar la evaluacin de la movilidad del semen equino, mediante los mtodos convencional y SCA (CASA. El semen de tres caballos de la raza criollo colombiano (tres eyaculados cada uno fue congelado, en un diluyente (4% de yema de huevo, 5% de dimetilformamida. La movilidad del semen descongelado, se determin de forma convencional por microscopia de contraste de fase y mediante el software SCA. Para el anlisis estadstico, se ajust un modelo lineal generalizado (GLM y se determin la asociacin entre las variables, a travs de anlisis de correlacin y regresin. Se hallaron promedios de movilidad total de 54,3 10,1 y 61,8 13,9, para evaluacin convencional y por el sistema SCA, respectivamente. No se encontr diferencia significativa entre los mtodos de anlisis. Se concluye que el anlisis de semen equino, mediante el mtodo convencional y el sistema SCA, es equiparable, para analizar la movilidad del semen equino criopreservado.

  3. Adjusting the number of spermatozoa in mini-straws by determination of the operative volume of bovine semen.

    Science.gov (United States)

    Dumont, Pascal

    2002-04-01

    The importance of the number of sperm per insemination on fertility has been well demonstrated in cattle. This number is usually calculated from the concentration in the extended semen and a theoretical value for the operative volume of semen delivered during insemination. The objective of this experiment was to investigate the usefulness of the measurement of the delivered volume of semen when estimating sperm numbers from frozen-thawed mini-straws, by comparing the results obtained with an analytical balance to the theoretical volume. The density of semen extended with Biociphos Plus and Triladyl was determined to be 1.033 g/ml using the gamma sphere method. This value was used to convert semen weight into operative volume. The effect of semen temperature at the time of weighing (37 degrees C versus 20 degrees C) was investigated on six semen batches, two technicians measuring the operative volume of 50 straws for each combination of temperature and semen batch (a total of 1200 weighings). The temperature effect was found to be insignificant, which allowed warm semen to be weighed before motility was assessed during routine quality control. The operative volume was then measured in straws routinely produced at 17 bovine Al centers (12-105 semen batches per center, mostly three straws from each batch, a total of 1912 measurements). The observed volumes were normally distributed around 198.7 microl, 98% measuring between 180 and 210 microl. The operative volume was significantly different among centers (from 192 to 205 microl, P < 0.0001) and among batches within centers (P < 0.0001). The S.D. among straws within batches was 3.4 microl. Some centers showed high variability in straw volume whereas others were more consistent. Determination of the operative volume of frozen-thawed mini-straws by weighing the delivered contents is an accurate method for estimation of the number of sperm per dose. PMID:12035983

  4. Comparison of Extraction Methods to Detect Porcine Circovirus-2 and Porcine Reproductive and Respiratory Syndrome Virus in Semen Samples

    Directory of Open Access Journals (Sweden)

    Won-Il Kim

    2012-01-01

    Full Text Available Since, Porcine Circo Virus-2 (PCV2 and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV are shed in semen for a long period of time after infection and artificial insemination is a common practice in pig farms, semen is an important source of spreading these viruses to naive populations. Therefore, rapid and accurate detection of PCV2 or PRRSV in semen could be critical at the standpoint of disease prevention and control. In this study, the performance of three different extraction methods on semen samples was compared to determine an optimal extraction method for semen samples to detect PCV2 or PRRSV. Seven or 115 semen samples were collected from the boars experimentally challenged with PRRSV or PCV2, respectively. These two sets of the semen samples were processed by three different extraction methods: High-Throughput Total Nucleic Acids Isolation kit (HT-TNA, High-Throughput Viral RNA Isolation kit with modified procedure (mHT-VR and DNeasy kit or RNeasy kit for PCV2 or PRRSV, respectively. Then, the efficiency of the extraction methods were compared by conducting the same real-time PCR for each virus. HT-TNA and mHT-VR kits were faster and more convenient to process semen samples and HT-TNA kit showed higher or compatible sensitivity as compared to the other two methods while all three methods demonstrated 100% specificity. In conclusion, the HT-TNA Extraction Method could significantly reduce testing time and effort to process semen samples and showed better sensitivity for detection of PCV2 and PRRSV in semen.

  5. Survival of chlamydiae in human semen prepared for artificial insemination by donor

    DEFF Research Database (Denmark)

    Thorsen, Poul; Møller, Birger R.; Halkier-Sørensen, Lars; From, E; Nielsen, Niels Christian

    1991-01-01

    after storage when examined by enzyme immunoassay (Chlamydiazyme). When examined by cell culture, four proved chlamydia- positive before storage and two afterwards. The results indicate that testing for C. trachomatis has to be performed from the urethra of all donors of semen used for artificial......Semen specimens from 21 men with urethral infection with Chlamydia trachomatis were tested for the presence of the organism before and after cryopreservation for 3 weeks of storage at -196 degrees C. Five specimens were chlamydia-positive before preservation and four of them were still positive...

  6. Intrauterine Neospora caninum inoculation of heifers and cows using contaminated semen with different numbers of tachyzoites

    OpenAIRE

    Serrano-Martínez, E.; Ferre, I.; Osoro, Koldo; Aduriz, G.; R.A. Mota; Martínez, A.; Pozo, I.; Hidalgo, C.O. (Carlos); Ortega-Mora, L. M.

    2013-01-01

    OBJECTIVE: To investigate the potential of different Neospora caninum tachyzoite doses to infect heifers (experiment 1) and cows (experiment 2) when administered in utero by artificial insemination via contaminated semen. METHODS: In experiment 1, five groups of 5, 7, 8, 9, and 5 cyclic heifers were hormonally synchronized and artificially inseminated with semen containing 0 (A, controls), 10(2) (B), 5 x 10(3) (C), 5 x 10(4) (D), and 5 x 10(5) (E) live N. caninum NC-1 isolate-tachyzo...

  7. Infectivity of porcine circovirus type 2 DNA in semen from experimentally-infected boars

    OpenAIRE

    Madson, Darin M.; Ramamoorthy, Sheela; Kuster, Chris; Pal, Narinder; Meng, Xiang-jin; Halbur, Patrick G.; Opriessnig, Tanja

    2008-01-01

    Porcine circovirus type 2 (PCV2) is an economically important pathogen. It has been demonstrated that PCV2 DNA can be detected in boar semen by PCR; however, the biological relevance of this is unknown. The objectives of this study were to determine if semen positive for PCV2 DNA is infectious (1) in a swine bioassay, or (2) when used for artificial insemination. For the first objective, 4-week-old pigs were inoculated intraperitoneally with PCV2 DNA-negative (bioassay-control; n = 3), PCV2a ...

  8. Semen Collection, Assessment and Processing for in vitro Fertilization in Dog – a Review

    Directory of Open Access Journals (Sweden)

    Ioana Hașegan

    2012-05-01

    Full Text Available The attainment of a feasible in vitro capacitation is clearly dependent on the maintenance of suitable energy levels ofmammalian spermatozoa. This is because of the fact that all the sperm changes related to capacitation, such as theincrease in tyrosine phosphorylation or changes in motility patterns, are energy-consuming. Canine semen can becapacitated and undergoes acrosome reaction in vitro and spermatozoa are able to fertilize homologous oocytes in invitro culture conditions. The aim of this paper is to describe the recent research of the authors in the field. Recent andclassical reviews and new trends regarding semen prelevation, evaluation and preparation for in vitro fertilization indog.

  9. Evaluation of buffalo semen by Trypan blue/Giemsa staining and related fertility in vitro

    OpenAIRE

    B. Gasparrini; Mariotti, E.; L. Attanasio; de Rosa, A.; R. Di Palo; Boccia, L.

    2010-01-01

    The aim of this work was to verify the feasibility of an easy, quick double staining technique for evaluation of frozen-thawed semen to predict the fertilizing capability in vitro of buffalo bulls. In Experiment 1, frozen-thawed semen from 6 bulls was stained with double Trypan blue/ Giemsa and the incidence of acrosome-intact live (AIL), acrosome-intact dead (AID), acrosome-lost live (ALL) and acrosome-lost dead (ALD) sperm was recorded. In Experiment 2, sperm from the same bulls were used t...

  10. Effects of He-Ne laser irradiation on the storage of turkey semen

    Directory of Open Access Journals (Sweden)

    S. Passarella

    2011-03-01

    Full Text Available Maintenance or improvement of sperm quality during storage could prevent the loss of fertilizing capacity associated with stored turkey semen. Therefore the optimization of stored turkey semen could be useful to breeder industry since the commercial production of this bird relies almost entirely on artificial insemination. Previous research have shown that He-Ne laser irradiation in mammalian sperm increased the motility (Stato, 1986, decreased the mortality, promoted the acrosome reaction, which have a pivotal role in assisted fecundating programmes as therapy for resolving infertility in domestic animals..........

  11. Caffeine intake and semen quality in a population of 2,554 young Danish men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Swan, Shanna H; Skakkebaek, Niels E; Rasmussen, Sanne; Jørgensen, Niels

    2010-01-01

    The authors examined the association between semen quality and caffeine intake among 2,554 young Danish men recruited when they were examined to determine their fitness for military service in 2001-2005. The men delivered a semen sample and answered a questionnaire including information about caf......: 92, 160), respectively, compared with 56 mill/mL (95% CI: 50, 64) and 181 mill (95% CI: 156, 210) in non-cola-drinkers, which could not be attributed to the caffeine they consumed because it was...

  12. Computer-assisted semen analysis parameters as predictors for fertility of men from the general population

    DEFF Research Database (Denmark)

    Larsen, L; Scheike, Thomas Harder; Jensen, Tina Kold; Bonde, Jens Peter; Ernst, Erik; Hjollund, N H; Zhou, Y; Skakkebaek, N E; Giwercman, Aleksander

    2000-01-01

    The predictive value of sperm motility parameters obtained by computer-assisted semen analysis (CASA) was evaluated for the fertility of men from general population. In a prospective study with couples stopping use of contraception in order to try to conceive, CASA was performed on semen samples...... from 358 men. A recently developed CASA system, Copenhagen Rigshospitalet Image house sperm Motility Analysis System (CRISMAS) was used for assessment of motility parameters. This system has an editing function which allows correction of tracks made by the computer. Probably due to this function, the...

  13. Some altered concentrations of elements in semen of workers exposed to trinitrotoluene.

    OpenAIRE

    Liu, H. X.; Qin, W H; Wang, G R; Yang, Z.Z.; Chang, Y X; Jiang, Q G

    1995-01-01

    OBJECTIVES--A cross sectional study was performed to find the concentrations of elements contained in the semen of workers exposed to trinitrotoluene (TNT). SUBJECTS AND METHODS--Semen of exposed workers in two TNT plants located in He-Nan Province in 1992 were examined. RESULTS--The average TNT concentrations in the workplace, except the packing site, were found to have exceeded the maximal allowable concentration (MAC, 1 mg/m3); skin contaminations of male workers exposed to TNT were higher...

  14. Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.

    Science.gov (United States)

    Mata-Campuzano, M; Soleilhavoup, C; Tsikis, G; Martinez-Pastor, F; de Graaf, S P; Druart, X

    2015-11-01

    The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.410(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.410(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.010(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen. PMID:26421370

  15. Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

    Scientific Electronic Library Online (English)

    Daniella A. J., Paula; Estefnia S., Andrade; Luis D. S., Murgas; Viviane O., Felizardo; Elissandra U., Winkaler; Walmes, Zeviani; Rilke T. F., Freitas.

    2012-09-01

    Full Text Available Este estudo avaliou a adio de antioxidantes vitamina E e glutationa reduzida no smen criopreservado de curimba (Prochilodus lineatus) e comparou soluo de bicarbonato de sdio e gua destilada como ativadores. O experimento foi conduzido na estao ambiental da CEMIG, em Itutinga-MG, entre Dezem [...] bro/2009 e Janeiro/2010. Smen de sete animais, com motilidade espermtica acima de 80%, foi diludo em solues crioprotetoras compostas por metanol 10% e lactose 15% em diferentes concentraes de antioxidantes: 50 (VE50), 100 (VE100) e 250 (VE250) M de vitamina E, 0,5 (RG5.5), 1,0 (RG1.0) e 1,5 (RG1.5) mM glutationa reduzida e uma soluo controle sem antioxidante. O smen foi diludo na proporo de 1:4 (100 L de smen: 400 L de soluo crioprotetora). A toxicidade das solues foi avaliada pela motilidade espermtica aps de 10 minutos em soluo. O restante do smen diludo foi armazenado em palhetas de 0,5 mL mantidos em vapor de nitrognio por 24 horas e estocado em cilindro de nitrognio lquido por quatro dias. As amostras foram descongeladas em banho-maria a 60C por 8 segundos e avaliada a taxa (%) e durao (s) pela ativao do smen com gua destilada e bicarbonato de sdio a 1%. No teste de toxicidade, observamos que os antioxidantes da vitamina E e glutationa, nas diferentes concentraes, no foram txicos para o smen do curimba (P>0,05). A durao da motilidade foi maior (P0,05). Assim, os antioxidantes vitamina E e glutationa reduzida no melhoram a qualidade do smen criopreservado de curimba, mas no causam efeitos txicos para o smen in natura e criopreservados por no diminuir sua qualidade durante a criopreservao. Abstract in english This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus) semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga- [...] MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7) with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing different concentrations of antioxidants: 50 (VE50), 100 (VE100) and 250 (VE250) M of vitamin E, and 0.5 (RG0.5), 1.0 (RG1.0) and 1.5 (RG1.5) mM of reduced glutathione. A solution without antioxidants was used as a control. The semen was diluted at a ratio of 1:4 (100 L semen:400 ?L cryoprotectant solution). The toxicity of the solutions was evaluated by investigating semen motility after 10 min in the solution. The rest of the diluted semen was placed into 0.5 mL straws maintained in nitrogen vapour for 24 hours and packed into a nitrogen liquid cylinder for four days. The samples were thawed in a water bath at 60C for 8 s and the rate (%) and duration (s) of semen activation with distilled water or sodium bicarbonate was evaluated. In the toxicity test, we found that vitamin E and reduced glutathione were not toxic to curimba semen at any of the tested concentrations (P>0.05). The duration of motility was longer (P0.05). Thus, the antioxidants vitamin E and reduced glutathione did not improve the quality of cryopreserved curimba semen, but they did not cause toxic effects to the semen in natura and they did not decrease its quality during cryopreservation.

  16. Infecciones de Transmisión Sexual en Semen: El Hombre como Vector de Transmisión Sexual Transmission Infections in Semen: Men as Vector Transmission

    Directory of Open Access Journals (Sweden)

    Tamara Viscarra A

    2013-03-01

    Full Text Available En los últimos años el estudio de las infecciones de transmisión sexual ha cobrado gran importancia debido principalmente al incremento de estas en parejas heterosexuales y hombres que tienen sexo con hombres. En mujeres existe mucha información de epidemiología y patogénesis de estas infecciones, sin embargo, en hombres la información es muy escasa debido a que la mayoría no presenta sintomatología. En los últimos años se ha evidenciado un creciente interés en el estudio del semen como vía de transmisión, debido principalmente a la afinidad de algunos patógenos con los espermatozoides. Dentro de los principales microorganismos infectantes en semen se encuentran Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Virus de la Inmunodeficiencia Humana tipos 1 y 2, Virus Herpes Simplex 1 y 2, Virus Papiloma Humano, Virus de la Hepatitis B y C, Citomegalovirus, Virus Epstein-Barr y Trichomonas vaginalis.Sexually transmitted infections study has become an important issue in these days, mainly due to the increment of heterosexual and men have sex with men partners of people. In women, there is a lot information about epidemiology and pathogenesis of these infections. However, the information is very limited in men, because most infected men are asymptomatic. In recent years, there has been an increasing interest in study of semen as a transmission way, due to the affinity of some pathogens to sperm. The most prevalent microorganisms infecting semen are: Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Human Immunodeficiency Virus Types 1 and 2 Herpes Simplex Virus 1 and 2, Human Papillomavirus, Hepatitis B and C virus, Cytomegalovirus, Epstein-Barr and Trichomonas vaginalis.

  17. Correlation between chemical composition of seminal plasma and sperm motility characteristics of Prussian carp (Carassius gibelio

    Directory of Open Access Journals (Sweden)

    M. Mehdi Taati

    2010-11-01

    Full Text Available The objectives of the present study were to determine the relationships between chemicalscompositions of seminal plasma with sperm motility traits in Prussian carp, Carassius gibelio (Bloch,1782. There were significant positive correlations between sperm movment duration and Ca+2 of semen.Also, a significant positive relationship was found between percentage of motile spermatozoa and Ca+2 ofsemen. On the other hand, Na+, Cl- and pH correlated negatively with sperm movment duration.Understanding of such correlations can be useful to evaluation of sperm quality and make media(extender for dilution of semen and improving sperm motility parameters of Prussian carp.

  18. Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC

    Directory of Open Access Journals (Sweden)

    Muhammad Rizal

    2008-03-01

    Full Text Available In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control. Supernatant of the second tube was removed (treatment B or without seminal plasma. Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C. Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS, live spermatozoa (LS, and intact plasma membrane (IPM was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6% was significantly (P<0.05 higher than that of: treatment B (31, 44.8 and 45.2% and treatment A (11, 15.6 and 14.8%. In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation.

  19. Evaluation of a semen extender from a bioenergetic perspective.

    Science.gov (United States)

    Froman, D P

    2015-02-01

    Four premises for sperm preservation were previously outlined. The present work tested 2 of these. The first premise was that sperm mobility phenotype affects procedural efficacy. Random bred roosters were phenotyped with the sperm mobility assay. A normal frequency distribution was observed with 35% (SD = 16.4) mobile sperm. Test subjects had values >51% (high) or between 19 and 35% (below average). Phenotypes were confirmed by repeated measure analysis. Ejaculates were pooled by phenotype. Sperm were washed by centrifugation through 12% (wt/vol) Accudenz. Washed sperm were suspended in Beltsville Poultry Semen Extender (BPSE) at 2 × 10 sperm/mL. Such sperm were stored at 10°C for 24 h. In the case of highly mobile sperm, an exponential decay was observed with a -intercept of 72% and an asymptote of 53%. In contrast, postwash values for below-average males decreased linearly from a -intercept of 31 to 17% after 24 h. A logistic decay was observed when sperm from high phenotype subpopulation males were extended with BPSE rather than washed before storage. Whereas -intercepts were equivalent between experiments, end points were not, that is, 53 vs. 17% mobile sperm. This difference was attributed to the extent of cytotoxic edema. The second premise tested was that the sperm mobility assay can predict the status of sperm cell mitochondria in response to sperm manipulation. Highly mobile sperm were washed and then suspended in either saline or glucose-free extender. Solution pH and osmolality were equivalent. Extender osmolality was controlled by replacing glucose with mannitol. Sperm were stressed by incubation at 2 × 10/mL at 20°C for 8 h. In each case, loss of sperm mobility approximated a logistic function. Whereas -intercepts were equivalent, the time at which loss of function was half maximal was prolonged with the extender ( mobility phenotype as well as the means by which the extender was used. To date, such effects have not been addressed in attempts to preserve chicken sperm in vitro. PMID:26020745

  20. A comparative evaluation of semen parameters in pre- and post-Hurricane Katrina human population

    Directory of Open Access Journals (Sweden)

    Caner Baran

    2015-01-01

    Full Text Available A natural disaster leading to accumulation of environmental contaminants may have substantial effects on the male reproductive system. Our aim was to compare and assess semen parameters in a normospermic population residing in the Southern Louisiana, USA area pre- and post-Hurricane Katrina. We retrospectively evaluated semen analyses data (n = 3452 of 1855 patients who attended the Tulane University Andrology/Fertility Clinic between 1999 and 2013. The study inclusion criteria were men whose semen analyses showed ≥ 1.5 ml volume; ≥15 million ml -1 sperm concentration; ≥39 million total sperm count; ≥40% motility; >30% morphology, with an abstinence interval of 2-7 days. After the inclusion criteria applied to the population, 367 normospermic patients were included in the study. Descriptive statistics and group-based analyses were performed to interpret the differences between the pre-Katrina (Group 1, 1999-2005 and the post-Katrina (Group 2, 2006-2013 populations. There were significant differences in motility, morphology, number of white blood cell, immature germ cell count, pH and presence of sperm agglutination, but surprisingly there were no significant differences in sperm count between the two populations. This long-term comparative analysis further documents that a major natural disaster with its accompanied environmental issues can influence certain semen parameters (e.g., motility and morphology and, by extension, fertility potential of the population of such areas.

  1. 9 CFR 98.33 - Ports designated for the importation of certain animal semen.

    Science.gov (United States)

    2010-01-01

    ... facilities for the entry of animal semen from Mexico: Douglas, Naco, Nogales, San Luis, and Sasabe, Arizona; Calexico and San Ysidro, California; Antelope Wells, Columbus, and Santa Teresa, New Mexico; Brownsville, Del Rio, Eagle Pass, El Paso, Hidalgo, Laredo, and Presidio, Texas. (d) Limited ports. The...

  2. Good semen quality and life expectancy:A cohort study of 43,277 men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Jacobsen, Rune; Christensen, Kaare; Nielsen, Niels Christian; Bostofte, Erik

    2009-01-01

    Fertility status may predict later mortality, but no studies have examined the effect of semen quality on subsequent mortality. Men referred to the Copenhagen Sperm Analysis Laboratory by general practitioners and urologists from 1963 to 2001 were, through a unique personal identification number,...

  3. Effect of boron supplementation on semen quality estimates in mature boars

    Science.gov (United States)

    The objective of the study was to determine the effect of dietary boron supplementation on sperm production and semen quality estimates in mature boars. Twelve crossbred boars (Landrace x Large White x Duroc x Hampshire) that were 2.5 +/- 0.2 years of age and 215 +/- 5 kg were randomly assigned to ...

  4. Increased prevalence of leukocytes and elevated cytokine levels in semen from Schistosoma haematobium-infected individuals

    DEFF Research Database (Denmark)

    Leutscher, Peter D C; Pedersen, Mette; Raharisolo, Clairette; Jensen, Jørgen Skov; Hoffmann, Steen; Lisse, Ida; Ostrowski, Sisse R; Reimert, Claus M; Mauclere, Philippe; Ullum, Henrik

    2005-01-01

    In this study, we investigated the seminal inflammatory response to egg infestation of the urogenital organs in 240 semen-donating men aged 15-49 years living in a Schistosoma haematobium-endemic area of Madagascar. In 29 subjects (12%) with excretion of > or =5 ova/ejaculate, leukocytospermia (>10...

  5. Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender

    Directory of Open Access Journals (Sweden)

    R.I Arifiantini

    2007-06-01

    Full Text Available Cryoprotective agents (CPAs, protect the sperm during cryopreservation. There are two general classes of CPAs, at first penetrating cryoprotectants, these pass through the sperm membrane and act both intracellular and extracellularly, and the second non-penetrating cryoprotectants, these act only extracellularly. The objective of this study was to evaluate the addition of different CPAs namely glycerol (G, combination of ethylene glycol with glycerol (EG and dimethilformamide (DMF using skim milk trehalosa extender. The semen collected from 3 stallions using artificial vagina twice aweeks. The semen was evaluated, centrifugated and diluted in skim milk extender supplemented with 50 mM trehalose and three different CPAs with the concentration of sperm were 200x106 ml-1. Extended semen was then packed into minitub 0.3 ml and equilibrated at 4oC for 2 hours, freeze in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196oC. After 24 hours, the semen was thawed at 37oC for 30 second. Results of this experiment indicated that the percentage of motile and viable sperm in skim trehalosa extender using DMF and glycerol better than combination of ethylene glycol and glycerol.

  6. Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil

    Directory of Open Access Journals (Sweden)

    Bruno H.L.S. Alves

    2013-11-01

    Full Text Available In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Brazil. Thirty-nine semen samples and 81 milk samples were submitted to DNA extraction using a commercially available kit and following the manufacturer's instructions. The polymerase chain reaction (PCR was then performed in accordance with protocols described in the literature. The results of the present study revealed the presence of Ma in the DNA of 17.9% (7/39 of the semen samples and 3.7% (3/81 of the milk samples. The results obtained in the present study confirm the elimination of the DNA of Ma in the semen and milk samples. The presence of this agent in goat flocks is considered very risky in terms of reproductive disorders and contagious agalactia outbreaks in the Northeast region of Brazil.

  7. Alterations in semen parameters in men w?th epilepsy treated with valproate

    Directory of Open Access Journals (Sweden)

    Hatice Kose-Ozlece

    2015-10-01

    Full Text Available Background: Besides the well-known adverse effects of valproate (VPA, disorders related to male reproductive functions have been reported. Furthermore, only a limited number of previous studies have reported the relationship between VPA dose and impairment of the hormonal axis and semen quality. A patient with reversible changes that occurred in the sperm parameters after a dose increment of VPA.Methods: A 34-year-old male patient who was diagnosed with juvenile myoclonic epilepsy almost 15 years ago was admitted to our clinic. His seizures responded well to high doses of VPA treatment.Results: As the VPA dose was increased, consecutive semen analyses were performed and averaged for each dose; the results showed a remarkable decline in the sperm count and a manifest loss of sperm motility. VPA treatment was gradually diminished and stopped; meanwhile, treatment with another antiepileptic (lamotrigin was initiated to control the patients seizures. Nine months later, the patients semen analysis was within normal ranges. After modification of the patients treatment regimen, he and his wife had a healthy baby.Conclusion: We suggest that VPA-dependent impairments in the hormone and semen analysis parameters were reversible after the termination of medical treatment, and that the VPA treatment did not cause permanent hormonal deregulation and, these side effects are dose dependent.

  8. Cryobiology and a new look at the preservation of stallion semen

    DEFF Research Database (Denmark)

    Grout, Brian William Wilson; Lehn-Jensen, Henrik; Petersen, Morten Rønn; Draper, Delene; Morris, John G.

    2009-01-01

      During freeze-preservation of high-viability ejaculates of horse semen the duration of the equilibration time for nucleated straws (achieved at -7 0C following induced nucleation and during controlled, slow cooling to -60 0C) has little impact on viability, measured using propidium iodide stain...

  9. Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation

    OpenAIRE

    Kumar, Dayanidhi; Salian, Sujith Raj; Kalthur, Guruprasad; Uppangala, Shubhashree; Kumari, Sandhya; Challapalli, Srinivas; Chandraguthi, Srinidhi Gururajarao; Krishnamurthy, Hanumanthappa; Jain, Navya; Kumar, Pratap; Adiga, Satish Kumar

    2013-01-01

    Background Cytogenetic studies have demonstrated that low levels of chronic radiation exposure can potentially increase the frequency of chromosomal aberrations and aneuploidy in somatic cells. Epidemiological studies have shown that health workers occupationally exposed to ionizing radiation bear an increased risk of hematological malignancies. Objectives To find the influence of occupational radiation exposure on semen characteristics, including genetic and epigenetic integrity of spermatoz...

  10. Cadmium may impair prostate function as measured by Prostate Specific Antigen in semen

    DEFF Research Database (Denmark)

    Andreucci, Alessandro; Mocevic, Emina; Jönsson, Bo A; Giwercman, Aleksander; Giwercman, Yvonne Lundberg; Toft, Gunnar; Lundh, Thomas; Bizzaro, Davide; Specht, Ina Olmer; Bonde, Jens Peter

    2015-01-01

    We investigated the association between cadmium in blood and the concentration of the prostate specific antigen (PSA) in semen, including the modifying effects of zinc or the CAG polymorphism in the androgen receptor (AR). Blood and semen samples were collected from 504 partners of pregnant women...... in Greenland, Poland and Ukraine. We found an inverse trend between cadmium and PSA (log(β) = -0.121, 95% confidence interval (CI): -0.213; -0.029, P = 0.0103) in Greenlandic men. Similar results were observed in men with a high number of CAG repeats (CAG 24) (log(β) = -0.231, 95% CI: -0.363; -0.......098, P = 0.0009). Inverse trends between cadmium and PSA were found when semen zinc concentrations were below the median value for men from Ukraine and Greenland. These outcomes suggest that cadmium may impair prostate function, as measured by PSA in semen, while high zinc levels and a low number of CAG...

  11. High Prevalence of TT Virus DNA in Human Saliva and Semen

    OpenAIRE

    Inami, Tomoko; Konomi, Nami; Arakawa, Yasuyuki; ABE, KENJI

    2000-01-01

    Using the PCR assay, we found a high prevalence of TT virus (TTV) DNA in saliva and semen from patients who were seropositive for TTV. This finding suggests that the presence of TTV in body fluids other than serum may affect the routes of viral transmission.

  12. Cytogenetic study in piglets and swine embryos originated from irradiated semen

    International Nuclear Information System (INIS)

    The chromosomal constitution of embryos and projects obtained by artificial insemination with semen submitted to a gamma radiation was studied. The effects of this treatment on the prolificity (litter size) and the changes caused by irradiation were correlated with embryonic deaths, occurrence of rearrangements and others chromosomal abnormalities that interface upon reproductive characteristics in swine. (L.L.L.)

  13. Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation.

    Science.gov (United States)

    Chelucci, Sara; Pasciu, Valeria; Succu, Sara; Addis, Daniela; Leoni, Giovanni G; Manca, Maria E; Naitana, Salvatore; Berlinguer, Fiammetta

    2015-04-01

    Soybean lecithin may represent a suitable alternative to egg yolk for semen cryopreservation in livestock species. However, additional studies are needed to elucidate its effects on spermatozoa functional properties. Semen collected from five Sarda bucks was cryopreserved in Tris-based extender and glycerol (4% v:v) with different supplementations. In a preliminary experiment, different soybean lecithin concentrations were tested (1%-6% wt/vol) and results in terms of viability, percentages of progressive motile and rapid spermatozoa, and DNA integrity after thawing showed that the most effective concentration was 1%. In the second experiment, semen was frozen in a Tris-based extender with no supplementation (EXT), with 1% lecithin (EXT LC), and 20% egg yolk (EXT EY). The effectiveness of these extenders was also compared with a commercial extender. The EXT EY led to the highest viability and motility parameters after freezing and thawing (P preserved spermatozoa functionality, as demonstrated by the higher fertilization rates compared with the other media (66.2 ± 4.5% for EXT LC vs. 32.7 ± 4.5%, 38.7 ± 4.5%, 39.6 ± 5.2% for EXT, EXT EY, and commercial extender; P egg yolk in goat semen cryopreservation, because it ensures higher fertilization rates and a better protection from membrane damage by cold shock. PMID:25595356

  14. Factors affecting the distribution and abundance of the flagellated alga Gonyostomum semen (Ehrenberg) Diesing in Finland

    OpenAIRE

    Sjnberg, Tuomo

    2014-01-01

    Limalev Gonyostomum semen (Ehrenberg) Diesing on nykyisin laajalle levinnyt ja runsas siimalev Pohjoismaiden jrvien planktonlevyhteisiss. Lev esiintyy etenkin humuspitoisissa jrviss ja se voi muodostaa runsaita kukintoja, jotka voivat haitata vedenpuhdistuslaitoksia ja uimareita. Jrven pieni koko, tummavetisyys, vhn hapan vesi ja tyypilliset ravinnepitoisuudet ja lajin kyttytymisen ominaispiirteet ovat olleet selityksin lajin runsastumiselle. Tss tutkimuksessa arvioidaan jr...

  15. Use of Aloe vera-based extender for chilling and freezing collared peccary (Pecari tajacu) semen.

    Science.gov (United States)

    Souza, A L P; Lima, G L; Peixoto, G C X; Silva, A M; Oliveira, M F; Silva, A R

    2016-05-01

    As an alternative for the conservation of collared peccary semen, this research aims at evaluating the use of Aloe vera (AV) extract as a cryoprotectant for semen chilling and freezing. Five ejaculates were divided in two aliquots that were diluted in Tris plus egg yolk (EY; 20%) or AV extract (20%) and chilled at 5 °C. In both treatments, an adequate semen conservation was achieved and values closer to 40% motile sperm with viability and osmotic response ranging from 20% to 40%, and normal morphology of 80% were found after 36 hours of storage. Moreover, 12 other ejaculates were diluted in Tris plus EY (20%) or AV extract (5, 10, or 20%) and glycerol (3%). Samples were frozen in liquid nitrogen and thawed after 1 week. After thawing, all the treatments containing EY or AV provided similar values for sperm morphology, viability, osmotic response, membrane integrity, sperm motility, amplitude of lateral head, beat cross frequency, and rapid, low, and static subpopulations, but the highest values for straightness and the lowest values for curvilinear velocity were found using 20% AV (P < 0.05). In conclusion, we found that AV extract at a 20% concentration could be used as an alternative substitute to EY in the formulation of Tris extenders for collared peccaries' semen chilling or freezing. PMID:26830302

  16. Recent adverse trends in semen quality and testis cancer incidence among Finnish men

    DEFF Research Database (Denmark)

    Jorgensen, N.; Vierula, M.; Jacobsen, R.; Pukkala, E.; Perheentupa, A.; Virtanen, H. E.; Skakkebk, N. E.; Toppari, J.

    2011-01-01

    Impaired semen quality and testicular cancer may be linked through a testicular dysgenesis syndrome of foetal origin. The incidence of testis cancer has been shown to increase among Finnish men, whereas there is no recent publication describing temporal trends in semen quality. Therefore, we...... carried out a prospective semen quality study and a registry study of testis cancer incidence among Finnish men to explore recent trends. A total of 858 men were investigated in the semen quality study during 1998-2006. Median sperm concentrations were 67 (95% CI 57-80) million/mL, 60 (51-71) and 48 (39......-60) for birth cohorts 1979-81, 1982-83 and 1987; total sperm counts 227 (189-272) million, 202 (170-240) and 165 (132-207); total number of morphologically normal spermatozoa 18 (14-23) million, 15 (12-19) and 11 (8-15). Men aged 10-59 years at the time of diagnosis with testicular cancer during 1954...

  17. Good semen quality and life expectancy: A cohort study of 43,277 men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Jacobsen, Rune; Christensen, Kaare; Nielsen, Niels Christian; Bostofte, Erik

    2009-01-01

    Fertility status may predict later mortality, but no studies have examined the effect of semen quality on subsequent mortality. Men referred to the Copenhagen Sperm Analysis Laboratory by general practitioners and urologists from 1963 to 2001 were, through a unique personal identification number,...

  18. Methylation patterns in fetal tissues generated from gilts inseminated with fresh or cryopreserved semen

    Science.gov (United States)

    Environmental influences, such as pollutants, climate, or diet, can alter the epigenetic configuration of gametes. The objective of our study was to evaluate differences in methylation activity of fetal placenta and liver from porcine pregnancies derived from fresh or frozen/thawed semen. Thirty cyc...

  19. East-West gradient in semen quality in the Nordic-Baltic area

    DEFF Research Database (Denmark)

    Jrgensen, Niels; Carlsen, Elisabeth; Nermoen, Ingrid; Punab, Margus; Suominen, Jyrki; Andersen, Anne-Grethe; Andersson, Anna-Maria; Haugen, Trine B; Horte, Antero; Jensen, Tina Kold; Magnus, ystein; Petersen, Jrgen Holm; Vierula, Matti; Toppari, Jorma; Skakkebaek, Niels E

    2002-01-01

    Denmark and Norway have a three-fold higher incidence of testicular cancer than Estonia and Finland. Groups of young men from Denmark, Norway, Finland and Estonia were investigated to elucidate whether semen parameters and other related parameters follow a gradient between these countries, as doe...

  20. Is maternal obesity related to semen quality in the male offspring? A pilot study

    DEFF Research Database (Denmark)

    Ramlau-Hansen, Cecilia Høst; Nøhr, Ellen Aagaard; Thulstrup, A M; Bonde, Jens Peter; Storgaard, Lone; Olsen, Jørn

    2007-01-01

    BACKGROUND: Obesity is a strong predictor of fecundity and maternal obesity may well program semen quality during pregnancy, but to our knowledge, no published studies have evaluated this hypothesis. METHODS: From a Danish pregnancy cohort established in 1984-87, 347 out of 5109 sons were selected...

  1. Apoptotic markers in semen of infertile men: association with cigarette smoking

    Scientific Electronic Library Online (English)

    Nagla T., El-Melegy; Mohamed-Esam M., Ali.

    2011-08-01

    Full Text Available OBJECTIVES: (i) To examine the role of apoptosis in the pathogenesis of DNA damage in semen from infertile men. (ii) To assess the effects of smoking on apoptotic markers and seminal parameters among infertile men. (iii) To assess the correlation of apoptosis with conventional semen parameters. MATE [...] RIALS AND METHODS: The study was carried out on 70 men with idiopathic infertility, divided into two groups: thirty infertile non smokers and forty infertile smokers. In addition to 60 fertile men (30 non smokers and 30 smokers) as control group. Each subject provided semen for analysis of parameters, determination of % of DNA fragmentation, s-Fas, caspase-3 activity levels and cotinine levels. RESULTS: The results revealed that infertile men, particularly smokers have significantly lower semen variables and significantly higher levels of apoptotic variables (% of DNA fragmentation, s-Fas and caspase-3 activity) in addition to cotinine. CONCLUSIONS: The present findings provide additional evidence supporting the importance of the evaluation of apoptotic markers to test male infertility particularly among smokers.

  2. The Correlation between Ultrasound Testicular Volume and Conventional Semen Parameters in Albanian Subfertile Males

    Directory of Open Access Journals (Sweden)

    Adrian Kristo

    2014-09-01

    CONCLUSION: Testicular volume has a direct correlation with semen parameters and the critical total testicular volume indicating normal testicular function is approximately 26.6 ml (the mean testicular volume 13.3 ml. The measurement of testicular volume can be helpful for assessing fertility at the initial physical examination.

  3. Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review

    Directory of Open Access Journals (Sweden)

    Shailendra Chaurasia

    2013-06-01

    Full Text Available The low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches reveal that selection of sperm and antioxidant fortification play crucial role in improving the quality of semen. Different methods used for semen up-gradation like washing, sedimentation, swim up procedure and filtration like percoll gradient, glass wool, sephadex and sephadex ion exchanger with significant improvement in motility, Hypo osmotic swelling test reactivity, viability and acrosomal integrity. Antioxidants are added directly to extender at standard dose rate with positive result. Among different filtration column used sephadex ion exchanger (FS+IE was superior over other in improving the semen quality especially when fortified with Vitamin E. Moreover a complete protocol is required, which contain both antioxidant fortification and sperm selection simultaneously to handle the low grade semen from sub-fertile bulls. [Vet World 2013; 6(8.000: 579-585

  4. Effects of cell phone use on semen parameters: Results from the MARHCS cohort study in Chongqing, China.

    Science.gov (United States)

    Zhang, Guowei; Yan, Huan; Chen, Qing; Liu, Kaijun; Ling, Xi; Sun, Lei; Zhou, Niya; Wang, Zhi; Zou, Peng; Wang, Xiaogang; Tan, Lu; Cui, Zhihong; Zhou, Ziyuan; Liu, Jinyi; Ao, Lin; Cao, Jia

    2016-05-01

    Epidemiological and experimental evidence for detrimental effects of cell phone use on semen quality is still equivocal. And that recruiting participants from infertility clinic not from general population may raise the possibility of a selection bias. To investigate effects of cell phone use on semen parameters in a general population,We screened and documented the cell phone use information of 794 young men from the Male Reproductive Health in Chongqing College students (MARHCS) cohort study in 2013, followed by 666 and 568 in 2014 and 2015, respectively. In the univariate regression analyses, we found that the daily duration of talking on the cell phone was significantly associated with decreased semen parameters, including sperm concentration [β coefficient=-6.32% per unit daily duration of talking on the cell phone (h); 95% confidence interval (CI), -11.94, -0.34] and total sperm count (-8.23; 95% CI, -14.38, -1.63) in 2013; semen volume (-8.37; 95% CI, -15.93, -0.13) and total sperm count (-16.59; 95% CI, -29.91, -0.73) in 2015]. Internet use via cellular networks was also associated with decreased sperm concentration and total sperm counts in 2013 and decreased semen volume in 2015. Multivariate analyses were used to adjust for the effects of potential confounders, and significant negative associations between internet use and semen parameters remained. Consistent but nonsignificant negative associations between talking on the cell phone and semen parameters persisted throughout the three study years, and the negative association was statistically significant in a mixed model that considered all three years of data on talking on the cell phone and semen quality. Our results showed that certain aspects of cell phone use may negatively affect sperm quality in men by decreasing the semen volume, sperm concentration, or sperm count, thus impairing male fertility. PMID:26949865

  5. Concurrent quantification and comparative pharmacokinetic analysis of bioactive compounds in the Herba Ephedrae-Semen Armeniacae Amarum herb pair.

    Science.gov (United States)

    Song, Shuai; Chen, Feilong; Xing, Xuefeng; Ren, Mengyue; Ma, Qinhai; Xie, Ying; Tang, Qingfa; Luo, Jiabo

    2015-05-10

    The Mahuang-Xingren herb-pair (MX), the combination of Herba Ephedrae (Mahuang in Chinese) and Semen Armeniacae Amarum (Xingren in Chinese), is a classical combination used in traditional Chinese Medicine to treat asthma and bronchitis. A simple and reliable ultra-performance liquid chromatography-tandem mass spectrometry method was developed to simultaneously quantify and compare the pharmacokinetics of 5 ephedra alkaloids and epimers of amygdalin and prunasin in rat plasma after oral administration of Mahuang, Xingren, and MX aqueous extracts. Samples were pretreated by a single-step protein precipitation with acetonitrile, and diphenhydramine hydrochloride and puerarin were used as internal standards. Pharmacokinetic parameters were investigated using DAS 3.2.2 (Mathematical Pharmacology Professional Committee of China, Shanghai, China). The validated method demonstrated adequate sensitivity, selectivity, and process efficiency for the bioanalysis of 8 compounds, including 3 pairs of epimers. MX administration improved the bioavailability of amygdalin and prunasin. Furthermore, MX facilitated intake of lower doses of ephedra alkaloids and increased elimination rates in comparison with Mahuang alone. These results illustrate the rationale behind the preferred use of the combination of Mahuang and Xingren. To our knowledge, this is the first report of stereo-selective metabolism of amygdalin. Further, the metabolic mechanism underlying this phenomenon merits future research attention. PMID:25766850

  6. Studies on liquefaction and storage of ejaculated dromedary camel (Camelus dromedarius) semen.

    Science.gov (United States)

    Wani, N A; Billah, M; Skidmore, J A

    2008-12-01

    The purpose of this study was to evaluate seminal liquefaction and quality of ejaculated camel semen during storage in different extenders at room (23 degrees C) and refrigeration (4 degrees C) temperature. Semen was collected using an artificial vagina and diluted immediately (1:1), using a split-sample technique, in five extenders [(1) Tris-tes egg yolk, (2) Tris-lactose egg yolk, (3) citrate egg yolk, (4) sucrose egg yolk and (5) Tris-fructose egg yolk], while one fraction was kept without an extender to act as control. The semen was transported to the lab at 37 degrees C, in a portable incubator within half an hour, and thereafter liquefaction of semen was monitored every 15 min. After complete liquefaction of the semen it was evaluated for sperm concentration and morphology and then was extended to a final ratio of 1:3. Aliquots of each semen sample were then stored at refrigeration and room temperature. The average volume of an ejaculate was 4.3+/-0.4 mL and it had a very viscous consistency. The average concentration of spermatozoa was 230.4+/-10.7 x 10(6)mL(-1) and the proportion of spermatozoa with protoplasmic droplets averaged 1.02+/-0.2, while 2.7+/-0.6 and 9.7+/-2.9% had mid-piece and tail abnormalities, respectively. All extended semen samples liquefied within 1.5h at 37 degrees C, however, there was slow liquefaction in the sample without an added extender (control). Best liquefaction was observed in Tris-lactose extender followed by Tris-fructose and citrate egg yolk diluents whereas in the other two extenders there was head-to-head agglutination of the spermatozoa. There was no difference in the initial motility of the spermatozoa in extenders 1-5 after its liquefaction, however, after 24 and 48 h of storage a higher proportion of spermatozoa were motile in extenders 1, 2 and 4 (Psemen, when added to an extender (1:1) immediately after collection, liquefies within 60-90 min at 37 degrees C. It maintains a high proportion of motile and viable spermatozoa that can survive storage up to 48 h in Tris-lactose egg yolk, Tris-tes egg yolk and sucrose egg yolk diluents. However, best liquefaction and progressive sperm motility is achieved in Tris-lactose egg yolk extender. PMID:18082979

  7. Increase in post-thaw viability by adding seminal plasma proteins to Sanmartinero and Zebu sperm

    Directory of Open Access Journals (Sweden)

    Fabián L Rueda

    2013-06-01

    Full Text Available Background: cryopreservation decreases sperm viability by approximately 50%. Objective: the objective of this study was to determine the effect of the addition of seminal plasma proteins on post-thawing sperm viability in Sanmartinero and Zebu semen. Methods: semen samples from 10 bulls of each breed were used, and seminal plasma was subjected to two-dimensional electrophoresis to establish the relationship between the relative amount of each protein spot and sperm viability. Then, seminal plasma was subjected to exclusion chromatography to separate the fraction containing these proteins. This fraction was added in doses of 0.5, 1.0, 1.5 and 2.0 mg, to 1 x 10(6. Sperm was thawed and incubated at 37 °C for 1 h to determine its effect on postthaw viability. Sperm were frozen using two media (citrate-fructose-yolk and Bioxcell®. Results: we found one protein spot (16.20 kDa, PI 5.5 in Sanmartinero seminal plasma that correlated (r = 0.64 p<0.001 with viability. This spot was found in 21-25 chromatography fractions. The percentage of post-thaw viable sperm increased 20% (p<0.05 at 1.0 and 1.5 mg of the fraction when sperm was frozen using citrate-fructose-yolk; it increased 25% (p<0.01 with 0.5 mg when it was frozen with Bioxcell® media. Addition of 0.5 mg of the fraction to semen cryopreserved with Bioxcell® resulted in a greater (p<0.05 percentage increase of viable sperm in Sanmartinero semen (23 ± 8.3% compared with Zebu semen (6.0 ± 2.0%. Conclusions: these results show that seminal plasma proteins decrease cryopreservation damage in sperm. The effect depends on the cryoprotectant dose as well as the breed of bull.

  8. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    OpenAIRE

    Ehrlich Llasaca-Calizaya; Juvenal Napuchi-Linares; Lorgio Verdi-Olivares; Jesús Núñez-Rodríguez

    2015-01-01

    El objetivo fue estimar el tiempo de latencia (almacenamiento), para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®), los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacar...

  9. An Investigation of the Effects of Ivermectin on Blood Serum, Semen Hyaluronidase Activities and Spermatological Characteristics in Sheep

    OpenAIRE

    TANYILDIZI, Sadettin

    2002-01-01

    This study was carried out to investigate the effects of ivermectin on the hyaluronidase activities of blood serum and semen and on the sperm characteristics in sheep. In this investigation 18 sheep, at the age of 2 years and weighing between 50-60kg were used. After the administration of ivermectin subcutanously at a dose of 0.2 mg/kg, semen and blood samples were taken at different times. The hyaluronidase activities of blood serum and semen samples in sheep were determined to increase sign...

  10. Evaluacin de dos diluyentes para la criopreservacin de semen de caballos de la raza criollo colombiano

    Directory of Open Access Journals (Sweden)

    Giovanni Restrepo Betancur

    2014-01-01

    Full Text Available Introduccin. La composicin de los diluyentes para la criopreservacin del semen equino es determinante en la criotolerancia y la capacidad fecundante post-descongelacin de las clulas espermticas. Objetivo. Evaluar dos diluyentes para la congelacin de semen de caballos de la raza criollo colombiano. Materiales y mtodos. El semen de cuatro caballos de la raza criollo colombiano fue criopreservado mediante un protocolo de congelacin rpida con los diluyentes Equipro y Gent. La evaluacin de la movilidad del semen posdescongelacin se realiz mediante un sistema de anlisis computarizado de clase (SCA, con el cual se analizaron la movilidad total (MT, la movilidad progresiva (MP, la velocidad curvilnea (VCL, la velocidad lineal (VSL y la velocidad media (VAP. La vitalidad espermtica (VE y la morfologa normal (MN se evaluaron mediante tincin con eosina-nigrosina; la integridad de la membrana plasmtica se determin mediante la prueba hipo-osmtica (HOS. Para la evaluacin estadstica se ajustaron modelos lineales generalizados (GLM y las medias entre los tratamientos se compararon por la prueba de Tukey. Las asociaciones entre las variables estudiadas se evaluaron mediante un anlisis de correlacin de Pearson. Resultados. Se hall diferencia estadstica significativa (P?0.05 para la MT con medias de 50.9 % 19.4 % y 42.2 % 15.1 %, para Equipro y Gent, respectivamente. A excepcin de la VSL no se encontr diferencia entre los dems parmetros evaluados. Se encontr una alta correlacin entre los parmetros de movilidad, vitalidad e integridad de membrana. Conclusin. El diluyente Equipro produce tasas de movilidad total posdescongelacin superiores al diluyente Gent en el semen de caballos de la raza criollo colombiano.

  11. Use of cryotubes for the cryopreservation of tambaqui fish semen (Colossoma macropomum).

    Science.gov (United States)

    Maria, Alexandre Nizio; Carvalho, Allan Charles Marques; Araújo, Rafael Venâncio; Santos, Jadson Pinheiro; Carneiro, Paulo César Falanghe; Azevedo, Hymerson Costa

    2015-04-01

    Tambaqui (Colossoma macropomum) is a freshwater fish of great importance to aquaculture in several South American countries. Recent studies have developed a protocol for semen cryopreservation in 0.25 and 0.5 mL straws; however, this technique has limitations for fingerling production at a large scale due to the high fecundity of tambaqui. The main objective of this study was to evaluate the feasibility of using cryotubes (1.6 and 4.5 mL) for tambaqui semen cryopreservation. Semen samples were diluted in freezing solution (5% glucose solution, 10% methylglycol, 5% egg yolk), stored in 1.6 and 4.5 mL cryotubes, frozen in liquid nitrogen vapor at -175°C and transferred to a cryogenic container at -196°C. The cryotubes were thawed in a water bath at 60°C for 70 or 90 s and the motility (total motility - TM; progressive motility - PM; curvilinear velocity - VCL; straight line velocity - VSL and average path velocity - VAP) and the viability of sperm were evaluated. There was no significant difference in sperm motility and viability post-thawing between 1.6 and 4.5m L cryotubes, except for TM (47% and 40%, respectively). Thawing for 90 s provided better results, being used in fertilization trials. Although the fertilization rate did not differ between the cryotubes (41-45%), it was significantly lower than that for fresh semen (74%). A strong positive correlation was observed between the sperm motility and fertilization rate (r=0.69-0.89). We conclude that 1.6 and 4.5 mL cryotubes have high potential for tambaqui semen cryopreservation when thawed for a minimum time of 90 s at 60°C. PMID:25725470

  12. The use of semen evaluation and assisted reproduction in Spix's macaws in terms of species conservation.

    Science.gov (United States)

    Fischer, Dominik; Neumann, Daniel; Purchase, Cromwell; Bouts, Tim; Meinecke-Tillmann, Sabine; Wehrend, Axel; Lierz, Michael

    2014-01-01

    The Spix's macaw (Cyanopsitta spixii) is the rarest parrot on earth. The remaining captive population consists of 79 individuals. Captive propagation is ongoing to increase the number of individuals for future reintroduction back into the wild. Unfortunately, from 2004 to 2012, only 33 chicks hatched from 331 eggs. Semen evaluation and assisted reproduction might help to overcome this problem. Therefore, a recently developed electro-stimulated semen collection technique was used in Spix's macaws. Semen collection was successful in 39 of 78 attempts in 10 out of 17 males. Examination of the semen included evaluation of volume, color, consistency, contaminations and pH, as well as determination of motility, viability, morphology, concentration, and total count of spermatozoa. The median volume of semen samples was 5.6 µl. On average, 34.7 ± 21.9% (median 30%) of the sperm were motile and 23.1 ± 22.1% (median 16.5%) were progressively motile. In addition to spermatozoa, round cells were detected in the samples. Median sperm concentration was 15,500/µl (range 500-97,500/µl) and median viability was 50% (range 5-87%). Morphological examination revealed in 26.5% normal spermatozoa, high numbers of malformations of the head (50.2%) and tail region (20.5%), with 29% of all sperm showing multiple abnormalities. Artificial insemination was performed in three females; two eggs laid after artificial insemination had spermatozoa present on the perivitelline layer, suggesting the possible success of the insemination technique. Although no fertilization could be demonstrated, these preliminary results are promising, as they indicate that assisted reproduction might be a tool for species conservation in the Spix's macaw. PMID:24752991

  13. Association between environmental exposure to p, p'-DDE and lindane and semen quality.

    Science.gov (United States)

    Pant, Niraj; Shukla, M; Upadhyay, A D; Chaturvedi, P K; Saxena, D K; Gupta, Y K

    2014-09-01

    Scientific concern exists about the toxic effect of dichlorodiphenyldichloroethylene (p, p'-DDE) and lindane on male infertility, and the mechanism underlying male reproductive toxicity of this pesticide remains unanswered. We investigated not only the possible association between the chlorinated pesticide levels and semen quality in nonoccupationally exposed men, but also the probable mode of action using mitochondrial membrane potential (MMP), reactive oxygen species (ROS), lipid peroxidation (LPO), and sperm chromatin structure assay (SCSA). A study in 278 men (21-40 years old) who visited Obstetrics and Gynecology Department, KGMU, Lucknow, for semen analysis was conducted. We performed semen analysis according to the WHO guidelines, while p, p'-DDE and lindane analysis was done by the GLC and LPO by the spectrophotometer, and the sperm mitochondrial status, ROS, and SCSA with the flow cytometer. The questionnaire data showed no significant difference in the demographic characteristics between the two groups, i.e., trying to conceive >1 year and proven fertility. However, a significant difference in the concentration of p, p'-DDE and lindane was observed between the groups. When the subjects were divided among four categories by quartile of exposure, the subjects in the highest quartile showed low sperm motility as compared to the subjects in the lowest quartile. Pearson's correlation showed a significant negative correlation between semen p, p'-DDE, lindane level, and sperm quality and positive association with the number of cells with depolarized mitochondria, elevation in ROS production and LPO, and DNA fragmentation index (DFI). The findings are suggestive that these toxicants might cause a decline in semen quality, and these effects might be ROS, LPO, and mitochondrial dysfunction mediated. PMID:24793071

  14. Development of soya milk extender for semen cryopreservation of Karan Fries (crossbreed cattle).

    Science.gov (United States)

    Singh, V K; Singh, A K; Kumar, R; Atreja, S K

    2013-01-01

    Egg yolk based semen extenders are used widely, with the potential risk of xenobiotic contamination. This study was designed to develop a soya milk based extender to substitute egg yolk based extender for bovine semen cryopreservation. In the first experiment soya milk was prepared from fresh soya bean (Glycine max). Concentration of soya milk in tris based extender was standardized based on quality parameters of spermatozoa during liquid preservation at 5°C up to 72 h and compared with egg yolk tris (EYT) extender. Sperm in soya milk tris (SMT) extender with 25 percent soya milk showed no significant (P > 0.05) differences in all the quality parameters like motility, viability, membrane integrity and acrosome integrity, as compared to sperm in EYT extender up to 72h in liquid dilution. In the second experiment the Karan Fries semen was cryopreserved in SMT extender with 25 percent soya milk (selected from the first experiment) using different concentration of glycerol, as cryoprotectant, ranging from 6-7 percent with a difference of 0.2 percent to standardize optimum concentration based on post thaw motility of spermatozoa. Glycerol at a final concentration of 6.4 percent was found to be the best among all. Further, semen samples were split and cryopreserved in newly developed SMT extender containing 6.4 percent glycerol and compared with conventional EYT extender for post thaw sperm quality parameters and degree of cryocapacitation. There were no significant (P > 0.05) differences between sperm in EYT extender and SMT extender for post thaw motility, viability, membrane integrity, acrosome integrity and cryocapacitation. In conclusion, the newly developed SMT extender maintained comparable semen quality as compared to EYT extender hence it can. PMID:23435710

  15. Micoplasma hominis, Ureaplasma urealyticum y bacterias aeróbicas en el semen de hombres que consultan por infertilidad Micoplasma hominis, Ureaplasma urealyticum and aerobic bacteria present in the semen from men attending infertility service

    Directory of Open Access Journals (Sweden)

    Bertha Victoria Rodríguez Pendás

    2013-04-01

    Full Text Available Introducción: las infecciones en el semen humano pueden alterar la calidad espermática, y vincularse con problemas de infertilidad masculina. Objetivo: determinar la frecuencia de infecciones por Micoplasma hominis, Ureaplasma urealyticum y bacterias aeróbicas en el semen de hombres que consultan por infertilidad, e identificar si existe relación entre las infecciones encontradas y las alteraciones en las variables de calidad del semen. Métodos: se realizó un estudio descriptivo transversal, para evaluar muestras de semen de 140 hombres, con edades entre 20 y 45 años, provenientes de las consultas de infertilidad del Instituto Nacional de Endocrinología. Se realizó un espermograma completo, que incluyó leucocitospermia, siguiendo los lineamientos de la OMS, para determinar las variables cualitativas y cuantitativas del semen. Las muestras de semen fueron cultivadas en agar sangre y agar chocolate a 37° C en atmósfera de CO2 para investigar bacterias aeróbicas, y se utilizó un juego de reactivos (Mycoplasma System Plus que permite realizar el cultivo, la identificación, el conteo semicuantitativo y el antibiograma de micoplasmas/ureaplasma urogenitales. Se tuvo en cuenta los aspectos éticos, y los resultados obtenidos se analizaron mediante cálculo de por cientos y la aplicación de la prueba de chi cuadrado. Resultados: de las 140 muestras de semen evaluadas, 58 (41,4 % mostraron la presencia de infecciones, de ellas 37 correspondieron a Ureaplasma urealyticum (25,7 %, 2 a Micoplasma hominis (1,4 % y 19 a bacterias aeróbicas (13,8 %. Al comparar las variables cualitativas y cuantitativas del semen con los sujetos infectados y no infectados, no se observaron diferencias estadísticamente significativas en ninguna de las variables de calidad espermática evaluadas. Conclusiones: la frecuencia total de infecciones, en la muestra estudiada, fue relativamente alta, pero no asociada a alteraciones en las variables seminales.Introduction: human semen infections can alter the sperm quality and be associated to male infertility disorders. Objectives: to determine the frequency of infections from Micoplasma hominis, Ureaplasma urealyticum and other aerobic bacteria in the semen of men who attended the infertility service, and to identify whether there is some relation between the detected infections and the altered semen quality variables or not. Methods: a cross-sectional descriptive study was performed to evaluate semen samples from 140 men aged 20 to 45 years, who attended the infertility service at the National Institute of Endocrinology. According to the WHO guidelines, a complete spermiogram including leukocytospermia was performed in order to determine the qualitative and quantitative variables in the semen. The semen samples were cultured in blood agar and in chocolate agar at 37oC under CO2 environment to find out possible aerobic bacteria. To this end, a set of reagents known as Mycoplasma System Plus was used, allowing the culture, the identification, the semi-quantitative count and the antibiogram of urogenital mycoplasms/ureaplasms. The ethical aspects were allowed for; the results were analyzed through percentage estimations and the chi square test. Results: out of the 140 evaluated semen samples, 58 (41.4 % showed some infection, 37 of them were caused by Ureaplasma urealyticum (25.7 %, 2 by Micoplasma hominis (1.4 % and 19 by the aerobic bacteria (13.8 %. When making a comparison of the qualitative and quantitative variables of the semen from infected and non-infected subjects, there were not any statistically significant differences in the evaluated variables of the sperm quality. Conclusions: the total frequency of infections in the studied sample was relatively high, but was not associated to altered seminal variables.

  16. Effect of two protocols of cryopreservation on fertilizing capacity of stallion (equus caballus) semen / efecto de dos protocolos de criopreservación sobre la capacidad fecundante de semen equino (equus caballus)

    OpenAIRE

    Giovanni Restrepo Betancur; Juan Esteban Duque Cortés; Juan David Montoya Páez

    2012-01-01

    Abstract. Semen cryopreservation is a fundamental process for the development of biotechnologies for assisted reproduction in horses. The use of cryopreservation techniques with changes in concentrations and the nature of the cryoprotectant, as well as, the different types of vials for storage of semen, have become an alternative to improve the protocols used. The objective of this work was to evaluate the effect of two protocols of cryopreservation (freezing and vitrification) on the fertili...

  17. Evaluation of bison (Bison bison) semen from Yellowstone National Park, Montana, USA, bulls for Brucella abortus shedding.

    Science.gov (United States)

    Frey, Rebecca K; Clarke, P Ryan; McCollum, Matt P; Nol, Pauline; Johnson, Kammy R; Thompson, Brent D; Ramsey, Jennifer M; Anderson, Neil J; Rhyan, Jack C

    2013-07-01

    To determine if bison (Bison bison) bulls from Yellowstone National Park (YNP), Montana, USA, shed an infective dose of Brucella abortus in semen, 50 YNP bulls were captured on public lands in Montana during the winter and early spring (April-May) of 2010 and 2011. The bulls were immobilized, and blood and semen samples were collected for serology and Brucella culture. Thirty-five bulls (70%) were antibody-positive, and B. abortus was cultured from semen in three (9%) of the 35 antibody-positive or suspect bulls, though not at concentrations considered an infective dose. Eight bulls (six antibody-positive, two negative) had palpable lesions of the testes, epididymides, or seminal vesicles consistent with B. abortus infection. Breeding soundness exams and semen analysis suggested that antibody-positive bulls were more likely to have nonviable ejaculate (8/35; 23%) than bulls without detectable antibody (2/15; 13%). PMID:23778628

  18. Detection of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum by Multiplex PCR in Semen Sample of Infertile Men

    Directory of Open Access Journals (Sweden)

    M Golshani

    2007-08-01

    Full Text Available Background: The aim of this study was to detect Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum from semen samples of infertile men by Multiplex PCR and investigation of influence of bacteriospermia on semen parameters. Methods: Semen samples of 200 infertile men were evaluated by Multiplex PCR. In addition, analysis of semen parameters was performed according to the WHO guidelines. Results: All the patients were without clinical symptoms of urogenital tract infection. Thirty three percent of cases showed at least one bacterium. We found a noticeable relation between the presence of bacteriospermia and the rate of non motile and morphologically abnormal sperms (P 0.05. Conclusion: Asymptomatic existence of Chlamydia and Mycoplasmas in urogenital tracts might play an important role in sperm impairment due to infertility. Bacteriospermia can influence sperm's motility, morphology and concentration.

  19. The performance of Angora rabbit does and their progeny depending on the semen used for artificial insemination

    OpenAIRE

    Eiben, C.; Szendr, Z.; Allain, D.; Thbault, R.G.; Radnai, I.; Brn Nmeth, E.; Lanszki, J

    1999-01-01

    Eiben, C.; Szendr, Z.; Allain, D.; Thbault, R.; Radnai, I.; Brn Nmeth, E.; Lanszki, J. (1999). THE PERFORMANCE OF ANGORA RABBIT DOES AND THEIR PROGENY DEPENDING ON THE SEMEN USED FOR ARTIFICIAL INSEMINATION..

  20. The Drosera Extract as an Alternative In Vitro Supplement to Animal Semen: Effects on Bovine Spermatozoa Activity and Oxidative Balance

    OpenAIRE

    Eva Tvrdá

    2015-01-01

    In vitro storage and processing of animal semen is considered to be a risk factor to spermatozoa activity, possibly leading to reduced fertility and litter sizes following artificial insemination (AI). A variety of substances isolated from natural resources have the potential to exhibit protective or antioxidant properties on the spermatozoon, thus they may extend the lifespan of stored semen. Drosera (Drosera rotundifolia L.) has been shown to possess antimicrobial, anti-inflammatory and ant...

  1. Low semen volume in 47 adolescents and adults with 47,XXY Klinefelter or 46,XX male syndrome

    DEFF Research Database (Denmark)

    Aksglaede, L; Jrgensen, N; Skakkebaek, N E; Juul, A

    2008-01-01

    .0-43.8) years. Semen volume adjusted for duration of abstinence was significantly smaller in the patients [2.0 (0.2-5.7) mL] when compared with control group I [3.1 (0.3-12.5) mL, p <0.0001] and group II [3.6 (0.6-12.5) mL, p <0.0001]. There was no difference in semen volume between 47,XXY and 46,XX males. All...

  2. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2015-01-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  3. Effects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspius

    OpenAIRE

    Saeed Hajirezaee; Bagher M. Amiri; Ali R. Mirvaghefi

    2009-01-01

    Problem statement: Because of dramatic declines in stocks of endangered Caspian brown trout males, Salmo trutta caspius in Caspian Sea, each male brooder is stripped indispensably more than once during the spawning season in other to artificial insemination in hatchery. The aim of the present study was to assay the changes of indicators of semen quality (sperm motility, sperm production, semen volume and chemical composition of seminal fluid) during these sequential strippings. Approach: The ...

  4. Effects of Adding Adenosine Triphosphate to Semen Diluter on Quality of Spermatozoa of Fat-Tailed Sheep

    OpenAIRE

    E Pramono; TR Tagama

    2008-01-01

    The research aimed to investigate the effects of adding different levels of Adenosine Triphosphate (ATP) to semen diluter on quality of sperms of fat-tailed sheep. Treatments consisted of 5, 10 and 15 mg of ATP addition into semen diluter (Egg Yolk Sodium Citric). Results showed that treatments have no significant effects on spermatozoa motility, significant effects on spermatozoa viability, and high significant effects on spermatozoa mortality. On average, sperm motility for control was 79....

  5. Role of semen infected leukocytes in HIV mucosal transmission : Experimental model of SIVmac251 infection in Macaca fascicularis

    OpenAIRE

    Bernard-Stoecklin, Sibylle

    2013-01-01

    Human Immunodeficiency Virus (HIV) infection mostly spreads by the mucosal route: sexual transmission is the dominant mode of transmission, responsible for between 85% and 90% of cases of infection worldwide. These epidemiological data indicate that semen is one of the major sources of HIV-1 transmission. Semen, like other bodily secretions involved in HIV sexual transmission, contains the virus as two forms: cell-free viral particles and cell-associated virus, mostly in infected leukocytes. ...

  6. Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water

    OpenAIRE

    Garcia, G. W.; Mollineau, W. M.; Adogwa, A. O.

    2010-01-01

    This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 × 106 spermatozoa/ml. Experiment 1: 200 ejaculates were extended for liquid storage at 5 ∘ C and evaluated every day for 5 days to determine FPM% and its rate of deterioration. Experiment 2: 150 ejaculates were exte...

  7. Effects of In Vitro Zinc Sulphate Additive to The Semen Extender on Water Buffalo (Bubalusbubalis) Spermatozoa before and after Freezing

    OpenAIRE

    Kamran Dorostkar; Sayed Mortaza Alavi Shoushtari; Amir Khaki

    2014-01-01

    Background: The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. Materials and Methods: In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions from Iran, Urmia during summer and autumn 2011, 25 samples were used in each ...

  8. Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not

    OpenAIRE

    Vidament, Marianne; Vincent, P.; Martin, F.X.; Magistrini, Michèle; Blesbois, Elisabeth

    2009-01-01

    A suitable method for the cryopreservation of donkey semen would be very valuable for the ex situ management of genetic diversity in this species. This report uses a variety of observation and trials to evaluate the effect of cryoprotectants in per-cycle pregnancy rates (PC) in equids females (jennies (donkey) and mares (horse)). This was explored by (1) comparing the results of insemination of jennies and mares with cooled or frozen donkey semen, (2) examining the possible toxic effect of th...

  9. Introducing and validation of SYBR Green Real-Time PCR method to determinate sex ratio in bovine semen.

    Science.gov (United States)

    Maleki, Adham Fani; Moussavi, AliReza Heravi; Nassiri, Mohammad Reza; Tahmoorespur, Mojtaba; Vakili, Seyed Alireza

    2013-07-01

    Flow cytometry is a widely used application for validating the accuracy of sperm sexing. However, this method is relatively expensive and requires considerable technical support. An alternative method employing simpler technology at low cost could be suitable for the evaluation of bovine semen in laboratories with low budgets. We used a SYBR Green Real-Time PCR assay to determinate sex ratio in bovine semen. The PLP and SRY genes were amplified to isolate the specific fragments of X- and Y-chromosome sequences, respectively. Two certified standard curves were obtained using two plasmids containing PLP and SRY amplicons. Our results show no significant difference in semen sex ratio in unsorted semen (54.7±0.52% X and 47.6±0.60% Y). However, significant difference was observed in X/Y-sorted semen (93.3±0.08% X and 91.4±0.06% Y-sperm), as compared to the expected ratio in unsorted semen or the post-sorting reanalysis data. The evolution of X-chromosome bearing sperm content in unsorted samples showed an average of 52.6 for ejaculates and 51.8 for the commercial semen. In order to confirm our results, the accuracy, repeatability and reproducibility of the method were tested resulting in 98.2% accuracy, repeatability of CV=5.59% and reproducibility of CV=5.40%. Thus, this method is demonstrated to be a reliable and inexpensive way to test sexual chromosome content in semen samples. PMID:23773328

  10. Herpesvírus bovino tipo 1 no sêmen Bovine herpesvirus-1 in semen

    Directory of Open Access Journals (Sweden)

    Maurilio Andrade Rocha

    1999-06-01

    Full Text Available O herpesvírus bovino tipo 1 (HVB-1 é o agente causador da rinotraqueíte infecciosa bovina, além de estar associado a doenças do trato genital em bovinos. A transmissão do HVB-1 através da inseminação artificial (IA pode ocasionar problemas reprodutivos nas vacas inseminadas, como endometrite, infertilidade, absorção embrionária e abortos. Animais infectados tornam-se portadores vitalícios do HVB-1 e podem apresentar episódios intermitentes de reexcreção viral. O HVB-1 poder ser encontrado no sêmen de touros, independente do desenvolvimento de anticorpos neutralizantes. Uma vez que os testes sorológicos não são suficientes para se estimar a presença do HVB-1 no sêmen e que as condições de processamento e armazenamento do sêmen são ideais para a preservação do vírus, somente o exame individual das partidas pode assegurar a comercialização de sêmen livre do vírus. Testes laboratoriais para detecção do HVB-1 no sêmen bovino e medidas adicionais para controlar a transmissão do vírus através da IA são apresentados.Bovine herpesvirus 1 (BHV-1 is the causative agent of infectious bovine rhinotracheitis (IBR and is also associated with genital disease in cattle. BHV-1 transmission by artificial insemination (AI may cause reproductive problems in inseminated cows, such as endometritis, infertility, embryonic absorption and abortion. Infected animals are lifelong reservoirs of BHV-1 and may go through intermittent episodes of virus reexcretion. It is important to note that conditions of semen storage are optimal for virus survival. Additionally, BHV-1 can be found in bovine semen despite of the development of neutralizing antibody. Since serological tests are not sufficient to ascertain the presence of the virus in semen, the laboratory testing of all semen batches for BHV-1 is the only way to ensure the BHV-1-free status of the semen for commercialization. Laboratory tests used for BHV-1 detection in bovine semen and additional approaches to prevent the spread of BHV-1 through AI are presented.

  11. Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

    Directory of Open Access Journals (Sweden)

    Priscilla F Gerber

    2010-11-01

    Full Text Available The dynamics of porcine circovirus type 2 (PCV2 shedding in semen of naturally infected boars was studied. Semen was collected serially each 15 or 20 days during 62 days from 5 boars from a herd and from 11 boars from an artificial insemination center. All boars were positive for PCV2 DNA by nested polymerase chain reaction of raw semen in at least two sampling dates, and most of them had detectable shedding in all sampling dates. Real-time quantitative PCR was performed in 23 samples. All samples showed low amounts of PCV2 DNA, ranging from 98 to 652 PCV2 copies/mL. No differences between the frequencies of PCV2 DNA shed in semen were found considering herds and age of boars. PCV2 shedding in the semen can occur continuously or intermittently up to 60 days in naturally infected boars at 12 to 42 months old in absence of PCV2 clinical signs. These results demonstrate sporadic and long-term shedding patterns of low amounts of PCV2 DNA in semen from naturally infected boars.

  12. Influence of the thawing rate on the cryopreservation of semen from collared peccaries (Tayassu tajacu) using Tris-based extenders.

    Science.gov (United States)

    Castelo, T S; Bezerra, F S B; Souza, A L P; Moreira, M A P; Paula, V V; Oliveira, M F; Silva, A R

    2010-10-01

    The objective was to evaluate the influence of the thawing rate on the quality of frozen-thawed (cryopreserved in Tris-based extenders) semen obtained from collared peccaries (Tayassu tajacu). Semen from 13 sexually mature collared peccaries males were collected by electroejaculation, and evaluated for motility, vigor, sperm viability, membrane integrity, and sperm morphology. Semen was divided in two equal portions: the first was diluted in Tris-fructose and the other in Tris-glucose, with egg yolk (20%) and glycerol (3%) added to each portion. Extended semen was frozen in liquid nitrogen and thawed using two thawing protocols (37 degrees C for 1 min or 55 degrees C for 7 s, followed by an additional 30 s at 37 degrees C). There were no significant differences between the two extenders after extension, chilling, or glycerol addition. After thawing at 37 degrees C, there were 37.9 +/- 4.2% and 28.5 +/- 5.1% motile spermatozoa for samples extended in Tris-fructose and Tris-glucose, respectively, with 33.8 +/- 3.7% and 28.2 +/- 3.5% motile spermatozoa after thawing at 55 degrees C (no significant differences). Furthermore, there were no significant interactions between extenders and thawing protocols for any semen end point. In conclusion, semen from collared peccaries was successfully cryopreserved in Tris-based extenders and thawed with two protocols (37 degrees C for 1 min or 55 degrees C for 7 s). PMID:20580079

  13. Investigation the Effects of Dietary L-Carnitine Supplementation on Characteristics of Rooster Semen During Liquid Storage

    Directory of Open Access Journals (Sweden)

    Y.J. Ahangari

    2011-01-01

    Full Text Available The objective of present study was to investigate the effects of various levels of dietary L carnitine supplementation (0, 125, 250 and 500 mg kg‾1 on rooster semen characteristics during liquid storage. Semen were collected from 16 rooster using abdominal massage and suitable samples were mixed together and sperm characteristics including percentage of motile, viable, abnormal, semen pH, volume and concentration were assessed. This experiment was carried out on the basis of completely randomized design. Results showed that during liquid storage, the effect of L carnitine on motility and viability percentage of sperm in beltsville extender were significant (p1 L-carnitine supplementation. Semen characteristics such as volume, pH and abnormal percentage of sperm did not differ significantly (p>0.05. Furthermore, semen concentration of birds fed dietary carnitine significantly differ from controls during experiment (p1 L-carnitine. Therefore, use of L-carnitine supplementation (250 mg kg‾1 in broiler breeder male feeding is recommended to improve quality of rooster semen.

  14. Cloned embryos from semen. Part 2: Intergeneric nuclear transfer of semen-derived eland (Taurotragus oryx) epithelial cells into bovine oocytes

    Science.gov (United States)

    Nel-Themaat, L.; Gomez, M.C.; Pope, C.E.; Lopez, M.; Wirtu, G.; Jenkins, J.A.; Cole, A.; Dresser, B.L.; Bondioli, K.R.; Godke, R.A.

    2008-01-01

    The production of cloned offspring by nuclear transfer (NT) of semen-derived somatic cells holds considerable potential for the incorporation of novel genes into endangered species populations. Because oocytes from endangered species are scarce, domestic species oocytes are often used as cytoplasts for interspecies NT. In the present study, epithelial cells isolated from eland semen were used for intergeneric transfer (IgNT) into enucleated bovine oocytes and compared with bovine NT embryos. Cleavage rates of bovine NT and eland IgNT embryos were similar (80 vs. 83%, respectively; p > 0.05); however, development to the morula and blastocyst stage was higher for bovine NT embryos (38 and 21%, respectively; p cell-cycle resumption was observed at 16 h postactivation (hpa). For eland IgNT embryos, 13% had ???8 cells at 84 hpa, while 32% of the bovine NT embryos had ???8 cells at the same interval. However, 100 and 66% of bovine NT and eland IgNT embryos, respectively, that had ???8 cells synthesized DNA. From these results we concluded that (1) semen-derived epithelial cell nuclei can interact and be transcriptionally controlled by bovine cytoplast, (2) the first cell-cycle occurred in IgNT embryos, (3) a high frequency of developmental arrest occurs before the eight-cell stage in IgNT embryos, and (4) IgNT embryos that progress through the early cleavage stage arrest can (a) synthesize DNA, (b) progress through subsequent cell cycles, and (c) may have the potential to develop further. ?? 2008 Mary Ann Liebert, Inc.

  15. Variations of protein profiles and calcium and phospholipase A2 concentrations in thawed bovine semen and their relation to acrosome reaction

    Directory of Open Access Journals (Sweden)

    V. Alonso Marques

    2000-12-01

    Full Text Available Just as calcium plays an integral role in acrosome capacitation and reaction, several spermatozoon proteins have been reported as binding to the ovum at fertilization. We examined the relationship between thawed bovine semen protein profiles, seminal plasma calcium ion concentration, spermatozoon phospholipase A2 (PLA2 activity and acrosome reaction. Electrophoretic profile analysis of spermatozoa and bovine seminal plasma proteins (total and membrane revealed qualitative and quantitative differences among bulls. Variations in PLA2 and seminal plasma calcium concentration indicated genetic diversity among individuals. A 15.7-kDa membrane protein was significantly correlated (r = 0.71 with acrosome reaction, which in turn has been associated with in vivo fertility.Vrias protenas que constituem o espermatozide tm sido relatadas como sendo protenas que se ligam ao vulo no momento da fertilizao, bem como ons clcio tm um papel importante na capacitao e reao acrossmica. Baseado nisto, este estudo teve como objetivo analisar e correlacionar protenas do smen congelado bovino de diferentes raas, concentrao de ons clcio no plasma seminal e atividade da fosfolipase A2 do espermatozide com a reao acrossmica, visando encontrar fatores que influenciem no processo de fertilizao bovina. Anlises do perfil eletrofortico das protenas (totais e de membrana do espermatozide e do plasma seminal bovino revelaram variabilidade protica entre indivduos na qual diferenas qualitativas e quantitativas foram identificadas. A quantificao da fosfolipase A2, bem como da concentrao de clcio no plasma seminal revelaram diversidade gentica entre touros. Uma protena de 15,7 kDa apresentou correlao significativa (0.71 com a reao acrossmica, que pode estar diretamente relacionada com a fertilizao in vivo e deste modo outros experimentos podem ser realizados a fim de investigar a utilidade deste marcador protico na verdadeira fertilidade.

  16. Infecciones de Transmisión Sexual en Semen: El Hombre como Vector de Transmisión Sexual Transmission Infections in Semen: Men as Vector Transmission

    OpenAIRE

    Tamara Viscarra A; Priscilla Brebi M; Alejandra Andana V; Raúl Sánchez G

    2013-01-01

    En los últimos años el estudio de las infecciones de transmisión sexual ha cobrado gran importancia debido principalmente al incremento de estas en parejas heterosexuales y hombres que tienen sexo con hombres. En mujeres existe mucha información de epidemiología y patogénesis de estas infecciones, sin embargo, en hombres la información es muy escasa debido a que la mayoría no presenta sintomatología. En los últimos años se ha evidenciado un creciente interés en el estudio del semen como vía d...

  17. Cryopreservation of rabbit semen: effectiveness of different permeable and non-permeable cryoprotectants on post-thaw sperm quality and reproductive performances

    OpenAIRE

    Di Iorio, Michele

    2015-01-01

    Rabbit breeding for meat production is based mainly on artificial insemination (AI) programs. In order to obtain many of potential advantages of AI, improvement of the storage of rabbit semen is necessary. Therefore, meat rabbit farming would greatly benefit if semen could be stored after collection and subsequently used for AI without affecting fertility. The rabbit sperm can be stored by refrigeration (in liquid or solid extenders) or freezing. The use of frozen semen would provide more p...

  18. Prognostic value of a pre-freeze hypo-osmotic swelling test on the post-thaw quality of dog semen.

    Science.gov (United States)

    Karger, S; Geiser, B; Grau, M; Burfeind, O; Heuwieser, W; Arlt, S P

    2016-03-01

    Throughout cryopreservation, sperm are exposed to major osmotic challenges. Only intact membranes of sperm cells are able to regulate these volumetric changes, which can be determined by the hypo-osmotic swelling test (HOS test). Correlations between the HOS test and conventional semen variables are inconsistent. Therefore, the objectives of this study were (1) to examine relationships between HOS test results and standard semen variables before freezing and after thawing and (2) to evaluate the prognostic value of the HOS assessments on post-thaw quality of dog semen. Semen of 35 dogs was collected and analyzed before freezing and after thawing following a 7-day freeze-thaw interval. Conventional semen variables such as sperm cell motility, membrane integrity morphology were evaluated and the HOS test was conducted with results from this test being recorded. In fresh semen the HOS test was positively correlated with progressive motility of sperm cells: r=0.52, sperm cell membrane integrity: r=0.50 and normal sperm cell morphology: r=0.46 (P<0.05). In frozen-thawed semen, the data obtained with the HOS test were positively correlated with progressive sperm cell motility: r=0.67 and membrane integrity: r=0.86 (P<0.05). The data obtained with the HOS test in fresh semen were positively correlated with sperm cell membrane integrity: r=0.50 normal sperm cell morphology: r=0.55 and data from the HOS test (r=0.43; P<0.05) with frozen-thawed semen. For the prediction of individual cryopreservation capacity, results from assessment of the fresh semen variables of good and poor semen quality were statistically compared. Based on these results, it is not possible to predict the quality of frozen-thawed dog semen using the HOS test. PMID:26837622

  19. Hazard Analysis and Critical Control Points System for a Bull Semen Production Centre.

    Science.gov (United States)

    Goularte, K L; Madeira, E M; Ferreira, C E R; Duval, E H; Vieira, A D; Mondadori, R G; Lucia, T

    2015-12-01

    Bull semen production centres (SPC) generally present satisfactory quality control for sperm processing, but non-standardized hygiene procedures. This study describes a Hazard Analysis and Critical Control Points (HACCP) system developed for bull SPC and subsequently implemented in a commercial SPC. After the identification of hazards at each step of semen processing and the determination of their risk and severity, monitoring and corrective procedures were designed to assess the system's efficiency. The HACCP system identified six microbiological hazards, 10 physical hazards, four chemical hazards and three critical control points. After the establishment of Good Processing Practices, Standard Operating Procedures and Standard Sanitizing Operating Procedures, the system was validated through an audit, to identify eventual failures and to define measures to correct them. PMID:26477334

  20. Effects of alpha-lipoic acids on sperm membrane integrity during liquid storage of boar semen

    Directory of Open Access Journals (Sweden)

    Laura Parlapan

    2015-05-01

    Full Text Available Preliminary studies have shown that sperm membrane from swine shows high sensitivity to cryopreservation process, causing a dramatic reduction in sperm quality. This has been attributed to the production of reactive oxygen species, that cause lipid peroxidation in sperm membranes. The aim of the present study was to minimize the oxidative attack by adding different concentration of alpha-lipoic acid into the sperm liquid storage at 17C for 7 days. Freshly ejaculated boar semen was diluted with Beltsville Thawing Solution (BTS and supplemented with 5 levels of alpha-lipoic acid (0.015, 0.02, 0.05, 0.1, 0.15 mmol/ml. The membrane integrity was evaluated at days 0, 1, 3, 5 and 7 of liquid preservation, using flowcytometerFACSCanto II (BD Biociencias systems. The experiment indicate that supplementation of alpha-lipoic acid to the semen liquid storage extender improve sperm membrane

  1. Sperm Abnormalities in Post-thawed Semen of Tunisian Arab Stallions

    Directory of Open Access Journals (Sweden)

    M. Ben Mrad

    2013-01-01

    Full Text Available The study was undertaken in order to evaluate sperm morphology features of post-thawed semen of Tunisian Arab stallions. Forty two ejaculates was collected and frozen, during years 2009 and 2010, from 9 stallions aged between 9 to 24 years. After thawing, sperm morphology was studied after eosin-nigrosin stain. The percentages of abnormal head, mid piece, flagella, sperm with droplets and the total abnormal sperm were determined. Analysis of variance was carried out using SAS software. The results showed that all sperm morphology features varied among ejaculates within stallion and among stallions (p<0.01. The percentage of abnormal flagella and total abnormal sperm varied between young and old stallions (p<0.01. We concluded that freezing and thawing processes increased abnormal sperm that is due to cell alteration for old and young stallions, and consequently, the decrease of the quality of the thawed semen of Tunisian Arab stallions.

  2. COMPUTER-ASSISTED SEMEN ANALYSIS OF RAT SPERMATOZOA AFTER AN INTRAPERITONEAL ADMINISTRATION OF INSECTICIDE DIAZINON

    Directory of Open Access Journals (Sweden)

    R. TOMAN

    2013-12-01

    Full Text Available The aim of this study was to reveal the effect of diazinon on the rat spermatozoa motility characteristics using the computer-assisted semen analysis (CASA. Motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, STR, LIN, WOB, ALH, and BCF after the diazinon i.p. administration of 20 mg/kg b.w. were evaluated. 36 hours after the diazinon administration, only slight decrease in VCL, DCL and increase in percentage of progressive motility in the diazinon-treated group. Significant decrease (P<0.01 was only observed in BCF in diazinon-treated group. Computer-assisted semen analysis (CASA of rat sperm motility showed that acute diazinon administration slightly affected the rat sperm motility which can be the first step in the decreased fertilization capacity caused by pesticides. Further investigation of reproductive effects of diazinon is needed.

  3. Is semen loss syndrome a psychological or physical illness? A case for conflict of interest

    Directory of Open Access Journals (Sweden)

    Shivanand Kattimani

    2013-01-01

    Full Text Available Young men presenting with sexual problems arising out of non-contact like semen loss syndrome are common in the Indian subcontinent. They usually present with depressive, anxiety symptoms, and non-specific somatic complaints. This has no medical explanation and is currently conceptualized as a culture bound neurotic disorder in the International Classification of Diseases-10 (ICD-10, clinical descriptions, and diagnostic guidelines. In this report, we present the case of a young male who developed delusions following concerns about semen loss. Conflicting explanations about his illness between traditional and allopathic practitioners led to problems in management. The importance of creating awareness among traditional practitioners regarding contemporary allopathic models of illness is stressed. Management strategies employed should reflect this shared understanding.

  4. Two new types of chromosomal rearrangements in the swine species induced by semen irradiation

    International Nuclear Information System (INIS)

    In the present experiment were used one boar and 5 descendent of Landrace and Large White cross-breeding were used, all the animals were healthy concerning to the reproductive aspect and chromosome constitution. Initially semen was collected from the boar through the glove hand method, diluted and submitted to gamma irradiation. The total applied dose was of 800 R, with an exposition period of 3,76 min. The artificial insemination of the females with the treated semen was performed from the time of observation of positive tolerance reflex, with each animal receiving 2 inseminations with a 12 hour interval in between. after birth, the piglets had their blood aseptically collected for karyotype preparation and analysis. From 17 piglets born and cytogenetically analysed, 2 chromosomal rearrangements were detected, namely, a reciprocal translocation or insertion, 8q-; 14p+ in a female a pericentric inversion in chromosome 1 in a male. (author). 18 refs, 2 figs

  5. Human semen quality in relation to dietary pesticide exposure and organic diet

    DEFF Research Database (Denmark)

    Juhler, R. K.; Larsen, S. B.; Meyer, Otto A.; Jensen, N. D.; Spanò, M.; Givercman, A.; Bonde, J. P.

    1999-01-01

    The objective of the study was to corroborate or refute the hypothesis that farmers having a high intake of organic grown commodities have a high semen quality due to their expected lower level of dietary pesticides intake. Food frequency data and semen were collected from 256 farmers (171...... mean intake of single commodities, such as rice, potato, and pork meat. The current individual dietary intake of 40 pesticides was estimated using food frequencies and generalized serving size data in combination with data on pesticide concentrations in food commodities as obtained from the National...... Danish Food Monitoring Program. The estimated pesticide intake was significantly lower among farmers of group H, but for all three groups of farmers the average dietary intake of 40 pesticides was at or below 1% of the acceptable daily intake (ADI) except for the dithiocarbamates (max = 0.21 mu g/kg day...

  6. Effect of semen extenders on frozen-thawed boar sperm characteristics and distribution in the female genital tract after deep intrauterine insemination in sows.

    Science.gov (United States)

    Noguchi, Michiko; Yoshioka, Koji; Hikono, Hirokazu; Suzuki, Chie; Kikuchi, Kazuhiro

    2015-12-01

    We compared the effects of extenders of frozen-thawed semen on post-thaw sperm characteristics and the distribution of frozen-thawed spermatozoa in the female genital tract after fixed-timed deep intrauterine insemination (DIUI) in sows. Frozen semen samples were thawed and diluted in either modified Modena solution (mMS) or porcine fertilization medium (PFM) containing theophylline, adenosine and cysteine. Sperm quality, assessed in vitro based on motility using a computer-assisted sperm analyzer and the integrity of the plasma and acrosomal membranes using flow cytometry, was evaluated at 0.5, 1.5, 3 and 6h after thawing. Progressive motility and the percentage of spermatozoa with damaged acrosomal membranes in PFM were significantly better than in mMS throughout the 6h. Sows with estrus synchronized using prostaglandin F2 alpha, equine chorionic gonadotropin and human chorionic gonadotropin (hCG) were inseminated once with mMS- or PFM-diluted 510(8) frozen-thawed spermatozoa by DIUI at 34h after the hCG injection. At 4h after DIUI, reproductive tracts were recovered from 30 sows. There were significantly fewer polymorphonuclear leukocytes (PMNs) and more spermatozoa outside PMNs in the uterine horn after PFM treatment than with mMS. When 22 sows were administered DIUI with 1010(8) frozen-thawed spermatozoa at 36h after hCG, the pregnancy rates did not differ significantly between the mMS- (36%) and PFM- (64%) treated groups. Thus, PFM enhanced progressive sperm motility but increased sperm membrane damage compared with mMS; it also suppressed the migration of PMNs into the uterine lumen. PMID:26588890

  7. The effect of semen analysis factors regarding IUI with male factor infertility

    Directory of Open Access Journals (Sweden)

    A. Ghaseminezhad

    2008-01-01

    Full Text Available AbstractBackground and Purpose: There is evidence in literature that IUI is the first line treatment and the most cost-effective procedure for mild to moderate male factor sub-fertility, however, the relative influence of various semen characteristics with the likelihood of a successful outcome is controversial. This study is designed to show the correlation between semen parameters and the pregnancy rate in patients, with mild to moderate male factor sub-fertility and whose wives were normal and underwent hyper-stimulation, including IUI.Materials and Methods: From January 2005 to January of 2006, 95 couples with male factor infertility underwent 140 IUI cycles with husbands washed semen were included in this study .They were divided into two groups based on semen parameters, mild and moderate. Post- wash sperm parameters, type of infertility (primary and secondary, male/ female age and duration of infertility were evaluated and correlated with clinical pregnancy outcome.Results: The clinical pregnancy rate per cycle was 26 (18.5%; 15 (21.4% in mild group, while 11(15.7% in moderate group. The clinical pregnancy rate per couple was 27.3%.There were significant correlation between female age, duration of infertility, sperm concentration and progressive motility, and clinical pregnancy.Conclusion: Our findings suggest that post- wash sperm concentration and progressive motility, female age and duration of infertility are the most important factors for prediction of successful pregnancJ Mazand Univ Med Sci 2008; 18(65:10-18 (Persian

  8. Seasonal and Genotype Variations in Libido, Semen Production and Quality in Artificial Insemination Boars

    OpenAIRE

    Avis Joseph; Chukwuemeka Okere; Michael Ezekwe

    2005-01-01

    The aim of this study is to access libido (sex drive) and examine the quality of ejaculates collected from Yorkshire (Large White) and Landrace boars, taking into account the potential effects of season and breed over a test period of one year. Two sexually mature boars (one from each breed, Yorkshire and Landrace) were trained to mount a dummy and ejaculate. Libido scores were assigned to boars during each semen collection. Scores ranged from zero to four, with zero indicating minimum and fo...

  9. Genetic basis of semen traits and their relationship with growth rate in rabbits

    OpenAIRE

    Tusell, Llibertat; Legarra, Andres; Garcia-Tomas, M.; Rafel, O.; Ramon, J.; Piles, M.

    2012-01-01

    This work aims to estimate the genetic parameters of seminal and production traits in a paternal line of rabbits selected for ADG during the fattening period. The onsidered traits were male libido (Lib) defi ned as successful mounting of an artificial vagina; presence of urine (Ur) and calcium carbonate deposits (Ca) in the ejaculate; semen pH; individual sperm motility (IM); the suitability for AI of the ejaculate (Sui), which involves the subjective combination of several quality traits; th...

  10. Effect of homeopathic treatment used in commercial boar semen diluent on sperm viability

    OpenAIRE

    Mayra Assunpção; Marcelo Goissis; Nilson Roberti Benites; Flavia Regina Barros; Sergio Azevedo; Leoni Villano Bonamin; Erlete Rosalina Vuaden; Cidéli de Paula Coelho; Francisco Rafael Soto; José Antonio Visitin; Mariana Grocke Marques

    2012-01-01

    Background: It has been speculated that the homeopathic treatment of sperm cells in order to improve semen quality could be promising. However, few data is available and its use in spermatozoa requires investigation. It is well established that mitochondrial membrane potential is an important viability parameter of spermatozoa and it is intimately related to reproductive efficiency. In this manner, new technologies in order to improve the activity of sperm cells and, finally, the fecundity of...

  11. Mycoplasma agalactiae in semen and milk of goat from Pernambuco state, Brazil

    OpenAIRE

    Bruno H.L.S. Alves; José G. Silva; André R. Mota; Ana C. Campos; José W.P. Júnior; Sandra B. Santos; Rinaldo A Mota

    2013-01-01

    In goat and sheep flocks, mycoplasmosis is a disease that may cause severe economical losses associated with polyarthritis, mastitis, agalactia, conjunctivitis, pneumonia and reproductive failure. The latter may involve repeat breeding, granular vulvovaginitis, infertility and abortions. The aim of the present study was to assess the occurrence of Mycoplasma agalactiae (Ma) in semen and milk samples from naturally infected goat in the semiarid region from Pernambuco State, Northeast from Braz...

  12. The effect of Rift Valley fever virus Clone 13 vaccine on semen quality in rams

    Directory of Open Access Journals (Sweden)

    Geoff Brown

    2015-02-01

    Full Text Available Rift Valley fever (RVF is an arthropod-borne viral disease of importance in livestock and humans. Epidemics occur periodically in domestic ruminants. People in contact with infected livestock may develop disease that varies from mild flu-like symptoms to fatal viraemia. Livestock vaccination may assist in disease control. Rift Valley fever virus (RVFV Clone 13 is a relatively new vaccine against RVF, derived from an avirulent natural mutant strain of RVFV, and has been shown to confer protective immunity against experimental infection with RVFV. The hypothesis tested in the current trial was that rams vaccinated with RVFV Clone 13 vaccine would not experience a reduction in semen quality (measured by evaluating the percentage progressively motile and percentage morphologically normal spermatozoa in successive ejaculates relative to unvaccinated control animals. Ram lambs were screened for antibodies to RVFV using a serum neutralisation test. Animals without detectable antibodies (n = 23 were randomly allocated to either a test group (n = 12 or a control group (n = 11. Animals in the test group were vaccinated with RVFV Clone 13 vaccine. Daily rectal temperature measurements and weekly semen and blood samples were taken from all animals. Seven animals were eliminated from the statistical analysis because of potential confounding factors. Logistic regression analysis was performed on data gathered from the remaining animals to determine whether an association existed between animal group, rectal temperature and semen quality parameters. No correlation existed between the treatment group and values obtained for the semen quality parameters measured. There was no statistically significant post-vaccination decline in the percentage of live morphologically normal spermatozoa, or the percentage of progressively motile spermatozoa, either when assessed amongst all animals or when assessed within individual groups. A repeat study with a larger sample size and a more comprehensive pre-screening process may be indicated to avoid the inclusion of unsuitable animals.

  13. Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen

    OpenAIRE

    Plante, Geneviève; Lusignan, Marie-France; Lafleur, Michel; Manjunath, Puttaswamy

    2015-01-01

    Background Mammalian semen contains a family of closely related proteins known as Binder of SPerm (BSP proteins) that are added to sperm at ejaculation. BSP proteins extract lipids from the sperm membrane thereby extensively modifying its composition. These changes can ultimately be detrimental to sperm storage. We have demonstrated that bovine BSP proteins interact with major milk proteins and proposed that this interaction could be the basis of sperm protection by milk extenders. In the pre...

  14. Need of reevaluation of the parameters of semen straws to be used in artificial insemination programs

    OpenAIRE

    Angel, J.; I. Posada; Berdugo, J.

    2010-01-01

    In buffalo industry artificial insemination is being used in breeding programs of our country . It has limitations such us seasonality, difficult estrus detection and low pregnancy rates when compared with cattle. IATF programs using a single insemination show results from 10 to 50% pregnancy rate, little information is available about minimum requirements of spermatozoa for IA. The aim of this paper is to compare the pregnancy rates after using narual mating or frozen semen in a sincronizati...

  15. Caracterizacin seminal y ensayos preliminares de crioconservacin se Semen de Bagre Rayado (Pseudoplatystomas fasciatum - linnaeus 1766)

    OpenAIRE

    Guarnizo Pineda, Melissa

    2007-01-01

    La crioconservacin seminal es usada actualmente como una herramienta biotecnolgica para la conservacin de las especies, as como para el uso eficiente de los gametos dentro de los procesos de reproduccin artificial. El bagre rayado (Pseudoplatystoma fasciatum) es un silrido nativo de las cuencas del ro Orinoco y Amazonas, con alto potencial para la diversificacin de la piscicultura nacional. El objetivo del presente trabajo fue caracterizar el semen de bagre rayado y realizar ensayos p...

  16. HIV enhancing activity of semen impairs the antiviral efficacy of microbicides

    OpenAIRE

    Zirafi, Onofrio; Kim, Kyeong-Ae; Roan, Nadia R.; Kluge, Silvia F.; Müller, Janis A.; Jiang, Shibo; Mayer, Benjamin; Greene, Warner C.; Kirchhoff, Frank; Münch, Jan

    2014-01-01

    Topically applied microbicides potently inhibit HIV in vitro but have largely failed to exert protective effects in clinical trials. One possible reason for this discrepancy is that the preclinical testing of microbicides does not faithfully reflect the conditions of HIV sexual transmission. Here, we report that candidate microbicides that target HIV components show greatly reduced antiviral efficacy in the presence of semen, the main vector for HIV transmission. This diminished antiviral act...

  17. Effect of improved diet on semen quality and scrotal circumference in the ram

    OpenAIRE

    Kheradmand, Arash; Babaei, Homayoon; Ali Batavani, Rooz

    2006-01-01

    The aim of this investigation was to assess the effect of improved diet above maintenance requirement on reproductive parameters, including testicular size, semen volume, sperm concentration and viability. Twelve Bakhtiary rams were allocated to two groups of six animals and were fed during a 12-week experiment period with different diets which were designed to supply maintenance and above maintenance requirements (dry matter, energy and protein). Dry matter (DM), barley and soybean meal for ...

  18. Semen analysis parameters: Experiences and insight into male infertility at a tertiary care hospital in Punjab

    International Nuclear Information System (INIS)

    Objective: To determine the prevalence of low sperm count including oligospermia and azoospermia in male infertile population, and to assess the pattern and distribution of abnormal semen parameters in infertile men. Methods: The descriptive cross-sectional survey was carried out at the Department of Gynaecology and Obstetrics, Sharif Medical City Hospital, Lahore, from June 2009 to June 2010. A total of 500 consecutively consenting male partners of women fulfilling the inclusion criteria between 20 and 40 years of age were approached. Semen analysis was performed according to methods and standards defined by the World Health Organisation (WHO). Samples were categorised into normospermia, oligospermia and azoospermia on the basis of sperm count. After exclusion of azoospermic samples, normospermic and oligospermic samples were compared for ejaculated volume, pus cells, motility and morphology. SPSS 10 was used for statistical analysis. Results: Out of the 500 males approached, 104 (20.8%) had to be left out either because of their unwillingness or inability to pass semen. The study sample comprised of 396 (response rate 79.2%); normospermia was observed in 293 (73.99%) males, azoospermia in 59 (14.89%), and oligospermia in 44 (11.11%). The oligospermic samples had low ejaculated volume, but significantly higher percentage of non-motile sperms 62%+-23.9% and abnormal morphology 55%+-15.6% in comparison to normospermic samples (p 0.0001). Asthenospermia was observed in 37 (25.81%), teratospermia in 11 (3.26%) and oligoasthenoteratospermia in 4 (9.09%) of samples. Conclusion: Semen analysis is the cornerstone for the evaluation of infertility in men. Sperm concentration, motility and morphology are related to each other, factors that cause deterioration of one of them usually also have negative impact on the other two as well. (author)

  19. Effects of Methanolic Extract of Moringa Oleifera Leaves on Semen and biochemical Parameters in Cryptorchid Rats

    OpenAIRE

    Afolabi, Ayobami Oladele; Aderoju, Hameed Adeola; Alagbonsi, Isiaka Abdullateef

    2013-01-01

    While anti-oxidant effects of Moringa oleifera in much oxidative stress related diseases have been well reported, cryptorchidism on the other hand has been shown to cause oxidative stress. However, study is scanty on the likely role of Moringa oleifera in reducing cryptorchidism-induced oxidative stress in rats has not been studied. The present study looked into the effects of methanolic extract of Moringa oleifera leaves (MEMO) on semen and biochemical parameters in cryptorchid rats. Twenty ...

  20. Sexual behavior and its relationship with semen quality parameters in Sahiwal breeding bulls

    Directory of Open Access Journals (Sweden)

    Shushant Singh

    2015-06-01

    Full Text Available Aim: The study was conducted at Artificial Breeding Research Centre, NDRI, Karnal, to determine the sexual behavior and its relationship with semen quality parameters in Sahiwal breeding bulls. Materials and Methods: A total of 63 ejaculates were collected from six adult Sahiwal bulls (age ~47 mo and bwt ~466 kg, to study the relationship of sexual behavior and semen quality. The degree of association between different variables was estimated by Pearson’s correlation coefficient method. Results: The results depicted that, sexual aggressiveness showed significantly high positive correlation with libido score (LS and sexual behavior score (SBS. Reaction time (RT and total time taken in mounts (TTTM had a significant negative correlation with LS and SBS. Penile erection score and penile protrusion score (PPS both had a significant positive correlation with ejaculatory thrust score, mating ability score, and SBS. Results of correlation among seminal attributes and with sexual behavior depicted that ejaculate volume had positive significant correlation with initial progressive motility (IPM, sperm concentration (SCON, head abnormality, total abnormality, hypo-osmotic swelling test (HOST, acrosomal integrity (AI whereas, mass activity had positive significant correlation with IPM, SCON, non-eosinophilic spermatozoa count (NESC, HOST, AI, RT and TTTM and IPM had positive significant correlation with SCON, NESC, HOST, AI, and TTTM, whereas and HOST had positive significant correlation with AI. Among seminal attributes, SCON had a positive significant correlation with PPS where as head abnormalities had a positive significant correlation with RT and TTTM. Conclusion: It can be concluded that the relationship of sexual behavior and semen quality parameters are reflecting that the sexual behavior of individual bulls is important to harvest good quality and quantity of semen as desired type of sexual preparation can be provided.

  1. Effects of oncological treatments on semen quality in patients with testicular neoplasia or lymphoproliferative disorders

    OpenAIRE

    Di Bisceglie, Cataldo; Bertagna, Angela; Composto, Emanuela R; Lanfranco, Fabio; Baldi, Matteo; Motta, Giovanna; Barberis, Anna M; Napolitano, Emanuela; Castellano, Elena; Manieri, Chiara

    2013-01-01

    Pretherapy sperm cryopreservation in young men is currently included in good clinical practice guidelines for cancer patients. The aim of this paper is to outline the effects of different oncological treatments on semen quality in patients with testicular neoplasia or lymphoproliferative disorders, based on an 8-year experience of the Cryopreservation Centre of a large public hospital. Two hundred and sixty-one patients with testicular neoplasia and 219 patients with lymphoproliferative disor...

  2. Effect of varicocele on semen characteristics according to the new 2010 World Health Organization criteria: a systematic review and meta-analysis.

    Science.gov (United States)

    Agarwal, Ashok; Sharma, Reecha; Harlev, Avi; Esteves, Sandro C

    2016-01-01

    This study investigated the effects of varicocele on semen parameters in infertile men based on the new 2010 World Health Organization laboratory manual for the examination of human semen. Semen analysis results (volume, sperm count, motility, and morphology) were the primary outcomes. An electronic search to collect the data was conducted using the Medline/PubMed, SJU discover, and Google Scholar databases. We searched articles published from 2010 to August 2015, i.e., after the publication of the 2010 WHO manual. We included only those studies that reported the actual semen parameters of adult infertile men diagnosed with clinical varicocele and contained a control group of either fertile men or normozoospermic men who were not diagnosed with varicocele. Ten studies were included in the meta-analysis, involving 1232 men. Varicocele was associated with reduced sperm count (mean difference: -44.48 10 [6] ml-1 ; 95% CI: -61.45, -27.51 10 [6] ml-1 ; P manual edition used for semen assessment. We conclude that varicocele is a significant risk factor that negatively affects semen quality, but the observed pooled effect size on semen parameters does not seem to be affected by the WHO laboratory manual edition. Given most of the studies published after 2010 still utilized the 1999 manual for semen analysis, further research is required to fully understand the clinical implication of the 2010 WHO laboratory manual on the association between varicocele and semen parameters. PMID:26780872

  3. BLOOD SERUM TESTOSTERONE LEVEL AND ITS RELATIONSHIP WITH SCROTAL CIRCUMFERENCE AND SEMEN CHARACTERISTICS IN NILI-RAVI BUFFALO BULLS

    Directory of Open Access Journals (Sweden)

    M. SAJJAD, S. ALI, N. ULLAH1, M. ANWAR1, S. AKHTER AND S. M. H. ANDRABI1

    2007-04-01

    Full Text Available This study was aimed at determining the blood serum testosterone level and its relationship with scrotal circumference and physical characteristics of semen in Nili-Ravi buffalo bulls. Semen samples were collected weekly from three buffalo bulls of 14 years age for 12 weeks and were evaluated for physical characteristics i.e. ejaculatory volume, sperm motility, sperm concentration, pH and sperm abnormalities. Jugular blood samples were collected from each bull at weekly intervals and analyzed for serum testosterorse concentrations. Mean (+ SE blood serum testosterone level (ng/ml, scrotal circumference (cm, semen volume (ml, progressive sperm motility (%, sperm concentration (106/µl, semen pH and total sperm abnormalities (% observed were 0.69 ± 0.12, 34.6 ± 0.9, 3.59 ± 0.41, 51.53 ± 2.23, 0.99 ± 0.07, 7.01 ± 0.08 and 11.67 ± 0.90, respectively. Positive correlations between testosterone level and scrotal circumference (r=0.414 and ejaculatory volume (r=0.348 were observed. However, no correlation of testosterone level with sperm motility (r=0.145, sperm concentration (r=0.264, semen pH (r=-0.208 and total sperm abnormalities (r=-0.242 was found. Similary, ejaculatory volume did not show any correlation with sperm motility percentage (r=0.115, sperm concentration (r=0.045, semen pH (r=-0.015 and total sperm abnormalities (r=-0.135. Sperm motility percentage had positive correlation with sperm concentration (r=0.347 and negative correlation with semen pH (r=-0.670. Sperm concentration was negatively correlated with semen pH (r=-0.501. It was concluded that in 14 years old buffalo bulls the level of serum testosterone and scrotal circumference and ejaculatory volume were positively correlated. The other semen quality parameters including sperm motility, sperm concentration, semen pH and sperm abnormalities were not related with serum testosterone level.

  4. High-resolution melt analysis of DNA methylation to discriminate semen in biological stains.

    Science.gov (United States)

    Antunes, Joana; Silva, Deborah S B S; Balamurugan, Kuppareddi; Duncan, George; Alho, Clarice S; McCord, Bruce

    2016-02-01

    The goal of this study was to develop a method for the detection of semen in biological stains using high-resolution melt (HRM) analysis and DNA methylation. To perform this task, we used an epigenetic locus that targets a tissue-specific differentially methylated region for semen. This specific locus, ZC3H12D, contains methylated CpG sites that are hypomethylated in semen and hypermethylated in blood and saliva. Using this procedure, DNA from forensic stains can be isolated, processed using bisulfite-modified polymerase chain reaction (PCR), and detected by real-time PCR with HRM capability. The method described in this article is robust; we were able to obtain results from samples with as little as 1 ng of genomic DNA. Samples inhibited by humic acid still produced reliable results. Furthermore, the procedure is specific and will not amplify non-bisulfite-modified DNA. Because this process can be performed using real-time PCR and is quantitative, it fits nicely within the workflow of current forensic DNA laboratories. As a result, it should prove to be a useful technique for processing trace evidence samples for serological analysis. PMID:26470939

  5. Coenzyme Q10 and soyphosphatidylcholine in EK extender on preservation of Rhode Island Red poultry semen

    Directory of Open Access Journals (Sweden)

    Amit Kumar Nath

    2015-06-01

    Full Text Available The objective of this study was to evaluate the efficacy of EK extender alone or incorporation with CoenzymeQ10 (CoQ10 and/or soyphosphatidylcholine (SPC in poultry semen and their effects on seminal traits during temporal storage at 4⁰C for different time intervals (12 h, 24 h, and 36 h. Heterospermic pooled semen samples diluted (1:4 with EK, EK + SPC, EK+ CoQ10 and EK + SPC + CoQ10 extenders separately, preserved and different spermiogram were assessed. Various seminal traits within the same extender differ significantly (p<0.05 among different groups and with different time intervals of storage. CoQ10 and SPC in the EK extender exhibited favorable synergistic effect on sperm quality and were able to protect the male gametes against cold-stress up to 36h at 4⁰C. In this study, we concluded that incorporation of SPC and CoQ10 together in EK extender possess novel potentiality to maintain seminal quality during liquid storage of poultry semen at 4⁰C and for their safe transportation and further use for Artificial Reproductive technologies (ARTs.

  6. Effects of Alpha Lipoic Acids on Cattle Sperm Kinetics Using Computer Assisted Semen Analysis

    Directory of Open Access Journals (Sweden)

    Amat Aswadi Abd Karim

    2012-01-01

    Full Text Available This study investigated the protective effect of Alpha Lipoic Acid (ALA on animal sperm quality using Computer Assisted Semen Analysis (CASA. Fresh semen sample collected from adult Limousin bulls. The experiment involved five test groups and a control. Alpha lipoic acid with different concentrations (0.1, 0.05, 0,025, 0.0125 and 0.00625 mmol mL-1 incubated into semen from all test groups. They were cryopreserved and thawed after 1 h. CASA analysis prior to cryopresevation confirmed the baseline condition. While post-thawed investigation determined the changes in sperm quality between the various groups. CASA parameters used were percent motility, Average Path Velocity (VAP, ? sec-1, Curvilinear Velocity (VCL, ? sec-1, Straight Line Velocity (VSL, ? sec-1, Amplitude of Lateral Head displacement (ALH, ?, Beat Cross Frequency (BCF, Hz, Linearity (LIN, ratio of VSL/VCL and Straightness (STR, ratio of VSL/VAP. ALA statistically improved VAP, VCL, VSL, ALH and BCF particularly for ALA concentration > 0.05 mmol L-1. On the other hand, it did not influenced LIN and STR. As a conclusion, ALA influences semicircular sperm motion and increases velocity. It might be useful as an additive in the extender or cryoprotectant agent to improve sperm motility.

  7. The combined use of embryos and semen for cryogenic conservation of mammalian livestock genetic resources

    Directory of Open Access Journals (Sweden)

    Pizzi Flavia

    2005-11-01

    Full Text Available Abstract The objective of this empirical simulation study was to evaluate the use of a combination of semen and embryos in the creation of gene banks for reconstruction of an extinct breed. Such an approach was compared for banks with varying proportions of embryos on the basis of the amount of the material to be stored, time for reconstruction, maintenance of genetic variability, and probability of failure during reconstruction. Four types of populations were simulated, based on reproductive rate: single offspring, twinning, enhanced reproduction, and litter bearing. Reconstruction was simulated for banks consisting of different combinations of semen and reduced numbers of embryos (expressed as a percentage of the material needed for a bank containing exclusively embryos and ranging from 10 to 90%. The use of a combination of semen and embryos increased the number of insemination cycles needed for reconstruction and the level of genetic relatedness in the reconstructed population. The risk for extinction was unacceptably high when a very low proportion of embryos (

  8. Genital Tract Infection in Asymptomatic Infertile Men and Its Effect on Semen Quality

    Directory of Open Access Journals (Sweden)

    M Golshani

    2006-07-01

    Full Text Available Male urogenital tract infection plays an important role in men infertility. Asymptomatic bacteriospermia has been paid attention as a major cause of male infertility. The aim of this study was to microbiological investigation of semen sample of infertile men attending to infertility clinic and evaluation of the effects of bacteriospermia on semen quality. Eighty eight infertile men were evaluated by standard bacterial culture method. Standard semen analysis was performed according to WHO guidelines. Among total cases, 35.22% (31 cases showed at least one pathogen: 10.22% E.coli, 9.09% Coagulase Negative Staphylococci (Saprophyticcus, 6.81% Group B Streptococci, 5.88% Entrococci, 5.68% Candida sp., 2.27% Gonococci, 2.27% Staphylococcus aureus, 1.13% Klebsiella sp. and 1.13% Providencia sp. There was a significant relation between the bacteriospermia and the rate of no motile and morphologically abnormal sperms (P0.05. It seems that leukocytospermia is a poor marker to predict bacteriospermia.

  9. The protective effect of antioxidants on liquid and frozen stored ram semen

    Directory of Open Access Journals (Sweden)

    Csilla Budai

    2014-05-01

    Full Text Available This systematic review is focusing on the current literature in order to give an overview of the protective role of antioxidants in ram semen preservation. Throughout the sperm conservation process the unsaturated fatty acids of the spermatozoa membrane binds oxygen and evolves numerous peroxide bonds. The lipid peroxidation leads to unbalanced oxidative stress that causes different impairments of sperm cells, and acrosome loss. ,,Cold shock” also induces caspase cascade involved in apoptosis, DNA fragmentation and in overall it has a detrimental effect on the fertilizing capacity of spermatozoa. Nowadays the cryopreservation of semen is considered as a routine procedure in cattle. Despite the various advantages of the method, the recovery rate of live and intact spermatozoa still remains low in boar, dog and ram samples. Previously several studies highlighted that the addition of antioxidants could improve the survival and motility rates, because antioxidants acted as free radical scavengers and protected spermatozoa against reactive oxygen species (ROS. Enzymatic antioxidants as superoxide dismutase (SOD, catalase, glutathione peroxidase (GPX and non-enzymatic antioxidant molecules like tocopherol, ascorbic acid, pyruvate, resveratrol have a protective effect against membrane damage that occurs during semen preservation process.

  10. Optimizing human semen cryopreservation by reducing test vial volume and repetitive test vial sampling

    DEFF Research Database (Denmark)

    S. Fuglesang Jensen, Christian; Ohl, Dana A; Parker, Walter R; da Rocha, Andre M; Keller, Laura M; Schuster, Timothy G; Sonksen, Jens; Smith, Gary D

    2015-01-01

    OBJECTIVE: To investigate optimal test vial (TV) volume, utility and reliability of TVs, intermediate temperature exposure (-88°C to -93°C) before cryostorage, cryostorage in nitrogen vapor (VN2) and liquid nitrogen (LN2), and long-term stability of VN2 cryostorage of human semen. DESIGN......-1,000 μL), and long-term storage in LN2 or VN2. MAIN OUTCOME MEASURE(S): Optimal TV volume, prediction of cryosurvival (CS) in ART procedure vials (ARTVs) with pre-freeze semen parameters and TV CS, post-thaw motility after two- or three-step semen cryopreservation and cryostorage in VN2 and LN2. RESULT...... the same ejaculate were similar. Cryosurvival of the first TV in a series of cryopreserved ejaculates was similar to and correlated with that of TVs from different ejaculates within the same patient. Cryosurvival of the first TV was correlated with subsequent ARTVs. Long-term cryostorage in VN2 did...

  11. Semen analysis of Golden Retriever healthy dogs and those affected by muscular dystrophy.

    Science.gov (United States)

    Peres, M A; da Rocha, A M; Vannucchi, C I; Mendes, C M; Cavalcanti, P V; Nichi, M; Ambrosio, C E; Miglino, M A; Visintin, J A; D'Ávila Assumpção, M E O

    2014-04-01

    This study aimed to characterise canine flow cytometry semen analysis, as well as seminal reactive oxygen species dosage using the Golden Retriever breed as model of study. Moreover, we searched for the influence of muscular dystrophy in Golden Retriever dogs on semen parameters. Thirty-seven semen samples were obtained from healthy Golden Retrievers (n = 15) and from muscular dystrophy affected dogs (n = 22). Sperm-rich fractions were analysed by standardised breeding soundness examination in addition to the assay of fluorescence assisted cell sorting for acrosome integrity, mitochondrial activity and DNA fragmentation. Volume of ejaculate, per cent of motile spermatozoa and vigour were similar between groups; there were no differences in the per cent of minor and major defects. Integrity of acrosomal membrane, mitochondrial potential and sperm DNA fragmentation had no significant differences between groups either. Animals from control group had higher concentration of spontaneous seminal oxidative species in comparison with affected animals. Dogs affected by dystrophy had seminal parameters similar to those observed in healthy dogs except for the lower concentration of oxidative species. Future studies aiming to establish reference values for canine seminal parameters should be considered preferably with distinction of breeds. PMID:23463904

  12. Caffeine intake and semen quality in a population of 2,554 young Danish men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Swan, Shanna H; Skakkebaek, Niels E; Rasmussen, Sanne; Jørgensen, Niels

    2010-01-01

    The authors examined the association between semen quality and caffeine intake among 2,554 young Danish men recruited when they were examined to determine their fitness for military service in 2001-2005. The men delivered a semen sample and answered a questionnaire including information about...... caffeine intake from various sources, from which total caffeine intake was calculated. Moderate caffeine and cola intakes (101-800 mg/day and semen quality. High cola (>14 0.5-L bottles....../week) and/or caffeine (>800 mg/day) intake was associated with reduced sperm concentration and total sperm count, although only significant for cola. High-intake cola drinkers had an adjusted sperm concentration and total sperm count of 40 mill/mL (95% confidence interval (CI): 32, 51) and 121 mill (95% CI...

  13. Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

    Directory of Open Access Journals (Sweden)

    Domosławska Anna

    2015-04-01

    Full Text Available Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast and vitamin E (5 mg/kg per os for 60 d. The control group (15 dogs was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC, and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05 after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

  14. Semen characteristics of transwomen referred for sperm banking before sex transition: a case series.

    Science.gov (United States)

    Hamada, A; Kingsberg, S; Wierckx, K; T'Sjoen, G; De Sutter, P; Knudson, G; Agarwal, A

    2015-09-01

    Transwomen (TW) can now turn to cryopreserve spermatozoa before gender reassignment (GR). The objective is to assess semen quality of TW and evaluate adequacy for assisted reproduction technology (ART). Pre-freezing (PF) and post-thaw (PT) semen parameters of 2 and PF data of 27 TW who were referred for sperm banking in Cleveland Clinic/USA and Ghent Center/Belgium, before GR, were retrospectively analysed. The study period was between February, 2003 and October, 2011. We also evaluated adequacy of 24-h PT data for ART. PF data of 29 TW, mean age of 28.9 years, showed high incidence of oligozoospermia (27.58%), asthenozoospermia (31%) and teratozoospermia (31%). Mean sperm concentration was 46.9 10(6) /ml, mean per cent motility was 42.9 and mean per cent sperm morphology (Kruger's) was 7.98. The 24-h PT data, for 2 TW, showed mean motility 22.4%, mean total motile sperm count 13.7 10(6) and total motile sperm concentration 8.7 106/ml. Single patient had used the frozen spermatozoon for intrauterine insemination (IUI) of a surrogate mother resulting in birth of healthy newborn. It is concluded that poor PF and 24-h PT semen quality is frequently seen among TW. As such, considerable proportion of TW should use more expensive method of ART, for example IVF/ICSI rather than inexpensive IUI. PMID:25269748

  15. Complications and the effect of varicocelectomy on semen analysis, fertility, early ejaculation and spontaneous abortion

    Directory of Open Access Journals (Sweden)

    Shamsa Ali

    2010-01-01

    Full Text Available Varicocele is still an enigma. Its effects on semen analysis, fertility and, more re-cently, early ejaculation and spontaneous abortion in spouses are not yet fully understood. In this retrospective study, we evaluated these four parameters (semen analysis, fertility, early ejacu-lation and spontaneous abortion among spouses in relation to varicocele and varicocelectomy during a 13-year period. A total of 1,711 patients with varicocele underwent varicocelectomy by high inguinal method (251 cases, subinguinal method (1,375 cases, scrotal method (34 cases, and subinguinal method with local anesthesia (38 cases. Our complication rate was acceptable. Sperm count, motility and morphology increased three months post operation in 55, 51, and 46%, respectively (P value 0.000, 0.000, and 0.015, respectively. Paternity was 56% after one year of post varicocelectomy follow-up. Only 7 out of 82 azoospermic men had sperm in their semen after varicocelectomy and only one of them with mild spermatogenic hypoplasia became a father. The spontaneous abortion rate in the spouses of respondents was 59%. Early ejaculation improved in 75% of the respondents. In conclusion, varicocelectomy does not improve sperm parameters in all men, but it improves pregnancy rate, early ejaculation, and scrotal pain.

  16. Reproductive performance of sows inseminated with diluted semen treated with homeopathic medicine

    Directory of Open Access Journals (Sweden)

    Mayra Elena Ortiz Assumpção

    2010-03-01

    Full Text Available Aims: this study sought to assess the reproductive performance of sows inseminated with sperm treated with homeopathic medicines. Materials and methods: the semen of 2 sexually mature boars age 18 months Pietrain and Duroc cross-bred with similar genetic and reproductive performance were chosen, as well as 125 sows. Sixteen samples of semen were collected and standardized through semen evaluation. Three homeopathic preparations and a placebo (control were tested on the sperm (n=31/32 per group: Avena sativa 6cH, Pulsatilla nigricans 6cH and Avena sativa 6cH + Pulsatilla nigricans 6cH. Sows were inseminated 3 times with the same estrous diagnostic procedures. Results: there was significant difference (p<0.05 between Avena sativa 6cH and the other 3 groups regarding the return to estrus and parturition rate. There was no significant difference among the groups regarding the number of newborn piglets. Conclusion: these data suggest that homeopathic preparation Avena sativa may be used directly on sperm cells to improve the parturition rate in technified swine farms.

  17. Importance of frozen-stored human semen in the spread of gonorrhea.

    Science.gov (United States)

    Sherman, J K; Rosenfeld, J

    1975-11-01

    The sensitivity of Neisseria gonorrhea in both infected human semen and urine to routine handling and storage above and below the freezing temperature has been studied. It was found that gonococci in such physiologic media remain viable in storage under atmospheric conditions, in gauze-plugged shell vials or in sealed plastic straws, for 1 to 8 days at +22 degrees C. Furthermore, cryo-survival of gonococci was demonstrated after pretreatment at +22 degrees C; freezing to, and thawing from, -196 degrees C; and frozen storage at -196 degrees C for at least 18 months, with and without the cryo-protectant glycerol. The relationship between laboratory diagnostic testing, disease transmission by males, and therapeutic donor insemination (AID) was discussed. It was strongly urged that only frozen-stored semen should be used in AID, primarily because of the possible use of asymptomatic donors. Cryo-preservation would ensure adequate testing of each sample to be inseminated, preventing the transmission of pandemic gonorrhea via semen. PMID:810371

  18. Effects of oncological treatments on semen quality in patients with testicular neoplasia or lymphoproliferative disorders.

    Science.gov (United States)

    Di Bisceglie, Cataldo; Bertagna, Angela; Composto, Emanuela R; Lanfranco, Fabio; Baldi, Matteo; Motta, Giovanna; Barberis, Anna M; Napolitano, Emanuela; Castellano, Elena; Manieri, Chiara

    2013-05-01

    Pretherapy sperm cryopreservation in young men is currently included in good clinical practice guidelines for cancer patients. The aim of this paper is to outline the effects of different oncological treatments on semen quality in patients with testicular neoplasia or lymphoproliferative disorders, based on an 8-year experience of the Cryopreservation Centre of a large public hospital. Two hundred and sixty-one patients with testicular neoplasia and 219 patients with lymphoproliferative disorders who underwent chemotherapy and/or radiotherapy and pretherapy semen cryopreservation were evaluated. Sperm and hormonal parameters (follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, inhibin B levels) were assessed prior to and 6, 12, 18, 24 and 36 months after the end of cancer treatment. At the time of sperm collection, baseline FSH level and sperm concentration were impaired to a greater extent in patients with malignant testicular neoplasias than in patients with lymphoproliferative disorders. Toxic effects on spermatogenesis were still evident at 6 and 12 months after the end of cancer therapies, while an improvement of seminal parameters was observed after 18 months. In conclusion, an overall increase in sperm concentration was recorded about 18 months after the end of cancer treatments in the majority of patients, even if it was not possible to predict the evolution of each single case 'a priori'. For this reason, pretherapy semen cryopreservation should be considered in all young cancer patients. PMID:23542137

  19. Effects of oncological treatments on semen quality in patients with testicular neoplasia or lymphoproliferative disorders

    Science.gov (United States)

    Di Bisceglie, Cataldo; Bertagna, Angela; Composto, Emanuela R; Lanfranco, Fabio; Baldi, Matteo; Motta, Giovanna; Barberis, Anna M; Napolitano, Emanuela; Castellano, Elena; Manieri, Chiara

    2013-01-01

    Pretherapy sperm cryopreservation in young men is currently included in good clinical practice guidelines for cancer patients. The aim of this paper is to outline the effects of different oncological treatments on semen quality in patients with testicular neoplasia or lymphoproliferative disorders, based on an 8-year experience of the Cryopreservation Centre of a large public hospital. Two hundred and sixty-one patients with testicular neoplasia and 219 patients with lymphoproliferative disorders who underwent chemotherapy and/or radiotherapy and pretherapy semen cryopreservation were evaluated. Sperm and hormonal parameters (follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, inhibin B levels) were assessed prior to and 6, 12, 18, 24 and 36 months after the end of cancer treatment. At the time of sperm collection, baseline FSH level and sperm concentration were impaired to a greater extent in patients with malignant testicular neoplasias than in patients with lymphoproliferative disorders. Toxic effects on spermatogenesis were still evident at 6 and 12 months after the end of cancer therapies, while an improvement of seminal parameters was observed after 18 months. In conclusion, an overall increase in sperm concentration was recorded about 18 months after the end of cancer treatments in the majority of patients, even if it was not possible to predict the evolution of each single case ‘a priori'. For this reason, pretherapy semen cryopreservation should be considered in all young cancer patients. PMID:23542137

  20. Human semen refrigeration at + 4 degrees C: bio-kinetic characteristics.

    Science.gov (United States)

    Dondero, Franco; Rossi, Tiziana; Delfino, Michele; Imbrogno, Norina; Cannistrà, Stefania; Mazzilli, Fernando

    2006-01-01

    The aim of our study was to evaluate the bio-kinetic characteristics of human semen refrigerated for different periods and to compare the effects of refrigeration at +4 degrees C against cryopreservation of human sperm at -196 degrees C. Semen was obtained from 30 male partners of infertile couples (infertile subjects) with the following semen profile: sperm count >or=10 x 10(6)/ml; progressive motility >or=20%; atypical forms <70% and white blood cells <1.0 x 10(6)/ml. Fifteen normospermic subjects were also selected as controls (control subjects). The following tests were carried out on basal, refrigerated and cryopreserved sperm: a) sperm kinetic properties (by Superimposed Image Analysis System); b) the Hypoosmotic Viability Test (HVT) (combined Hypoosmotic Swelling and Viability Test). The results of the study showed that the percentage recovery of kinetic properties and of HVT were optimum for up to 48 h. After refrigeration for 72 h, a drastic decrease in straight motility recovery was observed. No significant differences were observed between cryopreservation and refrigeration at +4 degrees C for 48 h for motility or HVT recoveries in samples from control subjects. However, in infertile subjects, a significant decrease in straight progressive motility and HVT recoveries was observed in cryopreserved samples compared to those refrigerated for 48 h. Neither refrigeration nor cryopreservation led to the growth of pathogenic bacteria in any of the cases studied. Based on the above results, refrigeration could represent a useful alternative to the cryopreservation method. PMID:16511666

  1. Infectious pathogens potentially transmitted by semen of the black variety of the Manchega sheep breed: Health constraints for conservation purposes.

    Science.gov (United States)

    Ruiz-Fons, Francisco; González-Barrio, David; Aguilar-Ríos, Fernando; Soler, Ana J; Garde, José Julián; Gortázar, Christian; Fernández-Santos, María Del Rocío

    2014-10-01

    Conservation of genetic resources from endangered breeds may be conducted through germinal banks. Preservation of healthy samples is paramount to avoid preserving pathogens shed with germinal products. The black variety of Manchega sheep (BMS), and endangered breed endemic to south-central Spain, is the subject of a conservation program; a germinal bank has been recently established. However, several pathogens circulating in BMS flocks may be shed with semen and threaten BMS preservation. Therefore, we investigated the sanitary status of BMS flocks and semen samples from 4 of the 17 flocks in which this variety is bred worldwide. A serological screening for Maedi-Visna virus, bluetongue virus, Pestivirus spp., Brucella spp., Coxiella burnetii, Chlamydophila spp., Mycobacterium tuberculosis complex, Mycobacterium avium paratuberculosis, Anaplasma spp., Mycoplasma agalactiae, Toxoplasma gondii and Neospora caninum was performed to assess for pathogens potentially shed by semen. Semen samples from 11 of the 35 BMS rams and 4 samples from coexisting rams of the white variety (WMS) were analyzed by PCR to detect Maedi-Visna virus, C. burnetii, Anaplasma marginale, Anaplasma phagocytophilum and T. gondii. Maedi-Visna virus RNA was detected in 3 semen samples (2 BMS and 1 WMS) while C. burnetii DNA was detected in 3 samples from WMS rams. Pathogens that can be transmitted by semen were present in BMS flocks, and Maedi-Visna virus and C. burnetii showed the highest potential for transmission by artificial insemination. Our results point to the need of testing semen samples kept for conservation purposes of BMS before using them for artificial insemination. PMID:25066603

  2. TRIXcell+, a new long-term boar semen extender containing whey protein with higher preservation capacity and litter size

    Directory of Open Access Journals (Sweden)

    B.M. van den Berg

    2014-02-01

    Full Text Available It was the aim of the present study to test whey as protective protein for the sperm cell in the long-term boar semen preservation medium TRIXcell. Analyses of sperm cell motility using computer-assisted semen analysis (CASA indicated that the whey protein Porex has a similar protective effect as bovine serum albumin (BSA in maintaining viability of stored boar sperm. Boar sperm diluted in TRIXcell+ maintains commercially acceptable motility (>60% for 10 days, while swine sperm diluted in the semen preservation medium Beltsville Thawing Solution (BTS maintains commercially acceptable motility (>60% for 3-5 days for most boars. To test the on-farm fertility performance of TRIXcell+ compared to BTS, inseminations were started on 35 commercial pig production farms in the summer of 2006. During the period of July 2006 until July 2012 for each farm and each calendar year the mean farrowing rate and litter size for semen diluted in TRIXcell+ and stored for 3-5 days was found higher than that of semen stored for 1-2 days in BTS. Based on data gained from a total of 583.749 sows inseminated through the years 2006-2012, the mean farrowing rate for semen diluted in TRIXcell+ and BTS was 90.4 ± 4.0 and 87.9 ± 3.6, respectively, which is not significantly different. Based on the same data, the mean total number of piglets born alive for semen diluted in TRIXcell+ and BTS was 14.2 ± 0.7 and 13.6 ± 0.6, respectively, which is significantly different. We conclude that whey protein can effectively be used in the long-term preservation medium TRIXcell resulting in a higher litter size.

  3. Predictive value of semen parameters and age of the couple in pregnancy outcome after Intrauterine insemination

    Directory of Open Access Journals (Sweden)

    Marjan Sabbaghian

    2013-11-01

    Full Text Available Background: Intrauterine insemination (IUI is one the most common methods in infertility treatment, but its efficiency in infertile couples with male factor is controversial. This study is a retrospective study about correlation between semen parameters and male and female age with successful rate of IUI in patients attending to Royan Institute.Methods: A total of 998 consecutive couples in a period of 6 months undergoing IUI were included. They were classified into two groups: couples with successful and unsuccessful pregnancy. Main outcome was clinical pregnancy. Data about male and female ages and semen analysis including concentration, total sperm motility, class A motility, class B motility, class A+B motility and normal morphology was extracted from patients’ records. Semen samples were collected by masturbation or coitus after 2 to 7 days of abstinence. Their female partners were reported to have no chronic medi-cal conditions and have normal menstrual cycles.Results: One hundred and fifty seven of total 998 cycles (15.7% achieved pregnancy. The average of female age in successful and unsuccessful group was 28.95±4.19 and 30.00±4.56 years, respectively. Mean of male age was 33.97±4.85 years in successful group and 34.44±4.62 years in unsuccessful group. In successful and unsuccessful groups, average of sperm concentration was 53.62±38.45 and 46.26±26.59 (million sperm/ml, normal morphology of sperm was 8.98±4.31 (% and 8.68±4.81 (%, sperm total motility was 47.24±18.92 (% and 43.70±20.22 (% and total motile sperm count was 80.10±63.61 million and 78.57±68.22 million, respectively.Conclusion: There was no significant difference in mean of females’ age and males’ age between successful and unsuccessful groups (P<0.05. In addition, there was no significant difference in semen parameters including concentration, total sperm motility, class A motility, class B motility, class A+B motility and normal morphology between two groups. It was shown that common semen analysis and male and female ages cannot predict IUI outcome.

  4. Vitamin E and reduced glutathione in Prochilodus lineatus (curimba semen cryopreservation (Characiformes: Prochilodontidae

    Directory of Open Access Journals (Sweden)

    Daniella A. J. Paula

    2012-09-01

    Full Text Available This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga-MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7 with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing different concentrations of antioxidants: 50 (VE50, 100 (VE100 and 250 (VE250 µM of vitamin E, and 0.5 (RG0.5, 1.0 (RG1.0 and 1.5 (RG1.5 mM of reduced glutathione. A solution without antioxidants was used as a control. The semen was diluted at a ratio of 1:4 (100 ìL semen:400 ∝L cryoprotectant solution. The toxicity of the solutions was evaluated by investigating semen motility after 10 min in the solution. The rest of the diluted semen was placed into 0.5 mL straws maintained in nitrogen vapour for 24 hours and packed into a nitrogen liquid cylinder for four days. The samples were thawed in a water bath at 60°C for 8 s and the rate (% and duration (s of semen activation with distilled water or sodium bicarbonate was evaluated. In the toxicity test, we found that vitamin E and reduced glutathione were not toxic to curimba semen at any of the tested concentrations (P>0.05. The duration of motility was longer (P0.05. Thus, the antioxidants vitamin E and reduced glutathione did not improve the quality of cryopreserved curimba semen, but they did not cause toxic effects to the semen in natura and they did not decrease its quality during cryopreservation.Este estudo avaliou a adição de antioxidantes vitamina E e glutationa reduzida no sêmen criopreservado de curimba (Prochilodus lineatus e comparou solução de bicarbonato de sódio e água destilada como ativadores. O experimento foi conduzido na estação ambiental da CEMIG, em Itutinga-MG, entre Dezembro/2009 e Janeiro/2010. Sêmen de sete animais, com motilidade espermática acima de 80%, foi diluído em soluções crioprotetoras compostas por metanol 10% e lactose 15% em diferentes concentrações de antioxidantes: 50 (VE50, 100 (VE100 e 250 (VE250 µM de vitamina E, 0,5 (RG5.5, 1,0 (RG1.0 e 1,5 (RG1.5 mM glutationa reduzida e uma solução controle sem antioxidante. O sêmen foi diluído na proporção de 1:4 (100 µL de sêmen: 400 µL de solução crioprotetora. A toxicidade das soluções foi avaliada pela motilidade espermática após de 10 minutos em solução. O restante do sêmen diluído foi armazenado em palhetas de 0,5 mL mantidos em vapor de nitrogênio por 24 horas e estocado em cilindro de nitrogênio líquido por quatro dias. As amostras foram descongeladas em banho-maria a 60°C por 8 segundos e avaliada a taxa (% e duração (s pela ativação do sêmen com água destilada e bicarbonato de sódio a 1%. No teste de toxicidade, observamos que os antioxidantes da vitamina E e glutationa, nas diferentes concentrações, não foram tóxicos para o sêmen do curimba (P>0,05. A duração da motilidade foi maior (P0,05. Assim, os antioxidantes vitamina E e glutationa reduzida não melhoram a qualidade do sêmen criopreservado de curimba, mas não causam efeitos tóxicos para o sêmen in natura e criopreservados por não diminuir sua qualidade durante a criopreservação.

  5. Evaluación de dimetilacetamida como crioprotector para la crioconservación de semen de bocachico Prochilodus magdalenae

    Directory of Open Access Journals (Sweden)

    VJ Atencio

    2013-01-01

    Full Text Available Se evaluó dimetilacetamida (DMA como crioprotector, a tres concentraciones: 8% (0,85 M, 10% (1,07 M y 12% (1,29 M combinado con glucosa 6% (0.33 M y yema de huevo 12% para crioconservar semen de bocachico Prochilodus magdalenae. El semen fue diluido en proporción 1:4 en la solución crioprotectora, empacado en pajuelas de 2,5 mL y congelado en vapores de nitrógeno líquido (N2L durante 30 minutos y luego almacenados en un termo de N2L de 34 L. La concentración, movilidad total, progresividad y velocidad tanto en semen fresco (control como en semen crioconservado-descongelado se evaluó con ayuda de Sperm Class Analyzer (SCA. Las pajuelas fueron descongeladas en baño serológico a 60°C durante 45 segundos. La fertilidad y eclosión fueron evaluadas inseminando dos gramos de huevos (1540 huevos/g a razón de 320.000 spz/ovocito. El semen fresco registró mayores valores de movilidad total, progresividad total, velocidad curvilínea y velocidad lineal con respecto al semen crioconservado-descongelado (P 0,05. DMA 12% reportó los menores valores de todas las variables que evaluaron la calidad seminal. Con semen fresco se obtuvo fertilidad de 74,0 ± 5,3% y eclosión de 62,3 ± 3,1%; mientras que con semen crioconservado-descongelado con DMA 8% se obtuvo adecuada fertilidad (60,4 ± 8,4% y eclosión (50,1 ± 9,8%. Los resultados del estudio sugieren que la solución crioprotectora compuesta por DMA 8%, glucosa 6% y yema de huevo 12% es una alternativa viable para la crioconservación de semen de bocachico.

  6. CARACTERSTICAS DO SMEN CAPRINO DESCONGELADO APS A ADIO DE RINGER LACTATO, CITRATO DE SDIO E SOLUO TRIS / CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION

    Scientific Electronic Library Online (English)

    Jlio Cesar Oliveira, Dias; Madriano Christilis da Rocha, Santos; Jurandy Mauro, Penitente Filho; Gisele Dias, Oliveira; Vivian Rachel Araujo, Mendes; Antonio Bento, Mancio.

    2015-06-01

    Full Text Available A criopreservao do smen de grande importncia para diversas biotecnologias da reproduo, como Inseminao Artificial (IA), Produo de Embries In Vitro (PIV) e Injeo Intracitoplasmtica de Espermatozoides (ICSI). Avaliou-se a estabilidade e persistncia da motilidade e vigor dos espermatozo [...] ides, assim como alteraes da membrana plasmtica, aps a adio de Ringer com Lactato, citrato de sdio 2,92% ou soluo TRIS ao smen caprino descongelado. O smen foi coletado de dois bodes da raa Parda Alpina, realizando-se os procedimentos padres de anlise e criopreservao seminal. Aps a descongelao do smen, foram adicionados os diluentes Ringer Lactato, citrato de sdio 2,92% ou soluo TRIS, realizando-se os Testes de Termorresistncia (TTR), Supravital e Morfolgico. No TTR, somente o grupo a que foi adicionada a Soluo TRIS obteve motilidade e vigor por maior perodo (90 minutos; P 0,05). Concluiu-se que a adio das solues no permite uma grande persistncia da motilidade e vigor dos espermatozoides descongelados, porm a soluo TRIS poderia ser utilizada para expanso de doses seminais utilizadas em biotecnologias reprodutivas in vitro. Abstract in english Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and stren [...] gth of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology.

  7. Effect of non-sperm cells removal with single-layer colloidal centrifugation on myeloperoxidase concentration in post-thaw equine semen.

    Science.gov (United States)

    Ponthier, Jérôme; Teague, Sheila R; Franck, Thierry Y; de la Rebière, Geoffroy; Serteyn, Didier D; Brinsko, Steven P; Love, Charles C; Blanchard, Terry L; Varner, Dickson D; Deleuze, Stéfan C

    2013-12-01

    Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after lysis. Post-thaw semen contains MPO and its concentration is associated with decreased sperm motility. Recently, MPO concentration in post-thaw semen was shown to be associated with the presence of non-sperm cells (NSC). The objective of this study was to evaluate the effect of a single-layer colloidal centrifugation before cryopreservation on NSC and MPO concentrations in equine semen. The experimental design consisted of freezing semen with or without previous centrifugation through two concentrations of single-layer colloid media. Non-sperm cells and MPO concentrations were assessed in pellet and upper layer at each step of the procedure and MPO was detected in cells by immunocytochemistry. Single-layer colloid centrifugation decreased NSC and MPO concentrations in post-thaw semen. The MPO concentration was correlated with concentration of NSC in the upper layer of the supernatant. In post-thaw semen, with or without previous single-layer colloid centrifugation, MPO concentration was correlated with concentration of NSC. Overall, neutrophils were rarely observed and NSC were mainly epithelial cells or cellular debris, as demonstrated by MPO immunocytochemistry. At all steps of the semen processing and cryopreservation, MPO immunostaining was clearly identified only on NSC. In conclusion, our study shows that NSC present in fresh semen release MPO during freezing. PMID:24054552

  8. PCV2 Vaccination Prevented Clinical PCVAD and Reduced PCV2 Viremia and Semen Shedding in Boars Concurrently Infected with PCV2b and Mycoplasma hyopneumoiae

    Science.gov (United States)

    Introduction. It has been determined that porcine circovirus type 2 (PCV2) DNA is shed in semen of naturally and experimentally infected boars (1). Recently, it also has been shown that PCV2 DNA present in semen is infectious in a swine bioassay model (2). However, under experimental conditions the ...

  9. Use of sexed semen and its effect on conception rate, calf sex, dystocia, and stillbirth of Holsteins in the United States

    Science.gov (United States)

    Sexed-semen use for breeding Holstein heifers and cows in Dairy Herd Improvement herds was documented by frequency and percentage for parity and service number as well as for herd region, size, and milk yield. Year of breeding accounted for the most variation in the amount of use of sexed semen for ...

  10. Preservasi Semen Kambing Peranakan Etawa dalam Pengencer Tris dan Sitrat Kuning Telur dengan Penambahan Sodium Dodecyl Sulphate (THE PRESERVATION OF ETTAWA GRADE BUCK SEMEN IN TRIS AND CITRATE EGG YOLK DILUENTS SUPPLEMENTED WITH SODIUM DODECYL SULPHATE

    Directory of Open Access Journals (Sweden)

    Nur Hidayati

    2015-10-01

    Full Text Available This study was conducted to determineSDS concentration also to compare Tris egg yolk and citrateegg yolk on the quality of ettawa grade chilled semen. The study consist of two experiments. The firstexperiment was to determine the best SDS concentration in Tris egg yolk diluents and the second experimentwas to compare the SDS suplementation in tris and citrate egg yolk in the quality of ettawa grade chilledsemen. The semen were collected from three bucks, immediately after collection the semen evalutedmacro and microscopycally. Semen demonstrate more than 70% motility and less than 10% spermatozoaabnormality, individually divided into four tube, each tube was diluted in egg yolk supplemented with 0;0,025; 0,05; and 0.075% SDS then stored at 5°C for 72 hours. The quality of chilled semen was observefor motility and viability every 12 hours. Result demonstrated that 0.05% SDS was the best concentrationcompared to others. In the second experiment,the semen were diluted in four different diluents, it wereTris egg yolk (TEY, Tris egg yolk with 0.05% SDS (TEYS, citrate egg yolk (CEY and citrate egg yolk with0.05% SDS (CEYS. Result demonstrated that the mean value of motility and viability of spermatozoa inTEYSdiluents (67.08 ± 6.43% and77.07 ± 6.78% was higher (p<0.05 than CEYS (60.42 ± 9.05% and72.31± 7.45%, TEY (59.23 ± 9.41% and71.21 ± 8.56% and CEY (53.45 ± 11.33% and67.74 ± 8.90%. In conclusion,supplementation of 0.05% SDS in TEY was maintained best sperm quality compared to other diluentsduring preservation.

  11. - Obtención de Semen de Rinoceronte Blanco (Ceratotherium simum simum mediante Condicionamiento Operante - (Semen Recollection of White Rinoceros (Ceratotherium simum simum With Operant Conditioning

    Directory of Open Access Journals (Sweden)

    Martínez del Castillo, Gerardo

    2006-08-01

    Full Text Available Existen animales sumamente valiosos dentro de las colecciones zoológicas, y en muchas ocasiones la complejidad que presentan dificulta su óptima reproducción en cautiverio. Hay gran variedad de causas por las que se modifican las conductas naturales y con esto se altera el ciclo biológico adecuado para su reproducción. La reproducción en cautiverio debe ser supervisada y contemplada de tal manera que no afecte ni dentro ni fuera de la colección, por lo tanto no siempre es sencillo contar con una variedad genética suficiente; esta es una de las razones por las que se realizan un gran número de intercambios entre instituciones, obteniendo así opciones para la óptima reproducción de los individuos de nuestra colección. Hoy en día se buscan alternativas para poder asistir a los animales que tengan antecedentes de poco éxito en reproducción; ya sea por su propia naturaleza, la ausencia o carencia de ciertos factores dentro de cautividad o por la falta de una pareja viable dentro de la colección. Una de estas alternativas es la recolección de semen de ciertas especies para incrementar la variedad genética de nuestra colección. Esta recolección se realiza en casi cualquier especie y estando el animal anestesiado, complicando la frecuencia con la que se puede llevar a cabo, además se necesita cierto equipo para contener y lograr la eyaculación del animal. Por esta razón es importante implementar un programa de entrenamiento con diferentes especies que se consideren difíciles de reproducir en cautiverio, como por ejemplo; los rinocerontes blancos, y así lograr la recolección del semen de una manera voluntaria y sin tener que contener al animal tanto física como químicamente. There are extremely valuable animals in the zoos collections, and in several occasions, due to their complexity, it is difficult their reproduction in captivity, because there are a lot of causes that modifies their natural behaviors and that alter the biological cycle adequated for their reproduction. The reproduction in captivity must be supervised and contemplated so it don’t affect inside or outside the collection, so it is difficult to count with enough genetic variety in the zoo, this is why exchanges between institutions are done, getting options to an optimum reproduction of the individuals of our collection. Today alternatives are search to help the animals that have little success in reproduction; due to their own nature, absence or deficiency of some factors in captivity or because of the lack of a viable partner inside the collection. One of the alternatives is semen recollection in some species, to increment the genetic variety of our collection, this recollection is usually done in almost all the species and the animal is anaesthetized, this complicated the frequency you can do the procedure, besides you require equipment to contain and get the animal’s ejaculation; due to this it is important to implement a training program with different species that are consider to be difficult to reproduce in captivity like the white rhinoceros, so the semen recollection can be done in voluntary way and without the necessity to contain the animal physical or chemically.

  12. Low semen volume in 47 adolescents and adults with 47,XXY Klinefelter or 46,XX male syndrome

    DEFF Research Database (Denmark)

    Aksglaede, L; Jørgensen, N; Skakkebaek, N E; Juul, A

    2008-01-01

    non-mosaic 47,XXY (n = 40) or SRY-positive 46,XX male (n = 7) karyotypes aged 26.1 (range: 15.0-51.7) years participated. Semen quality was compared with 2136 (control group I) men from the general population aged 18.9 (17.9-28.6) years and with 349 fertile men (control group II) aged 30.9 (22...... patients had azoospermia except two 47,XXY males aged 29 years who had sperm concentrations of 0.5 and 1.6 million/mL, respectively. We found significantly smaller semen volume in the patients when compared with controls, and the presence of motile spermatozoa in two out of 47 patients. The small semen...

  13. PFOS (perfluorooctanesulfonate) in serum is negatively associated with testosterone levels, but not with semen quality, in healthy men

    DEFF Research Database (Denmark)

    Joensen, Ulla Nordström; Veyrand, Bruno; Antignac, Jean-Philippe; Blomberg Jensen, Martin; Petersen, Jørgen Holm; Marchand, Philippe; Skakkebæk, Niels Erik; Andersson, Anna-Maria; Le Bizec, Bruno; Jørgensen, Niels

    2013-01-01

    semen samples. Serum samples were analysed for total testosterone (T), estradiol (E), sex hormone-binding globulin (SHBG), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and inhibin-B and 14 PFCs, including perfluorooctanesulfonate (PFOS). Semen samples were analysed according to the WHO......Is exposure to perfluorinated compounds (PFCs) associated with testicular function (reproductive hormone levels and semen quality) in healthy men? SUMMARY ANSWER: PFOS levels were significantly negatively associated with serum testosterone (total and calculated free), but not with any other...... criteria. MAIN RESULTS AND THE ROLE OF CHANCE: PFOS levels were negatively associated with testosterone (T), calculated free testosterone (FT), free androgen index (FAI) and ratios of T/LH, FAI/LH and FT/LH. Other PFCs were found at lower levels than PFOS and did not exhibit the same associations. PFC...

  14. Mathematical prediction of freezing times of bovine semen in straws placed in static vapor over liquid nitrogen.

    Science.gov (United States)

    Santos, M V; Sansinena, M; Zaritzky, N; Chirife, J

    2013-02-01

    A widespread practice in cryopreservation is to freeze spermatozoa by suspending the straws in stagnant nitrogen vapor over liquid nitrogen (N(2)V/LN(2)) for variable periods of time before plunging into liquid nitrogen (-196C) for indefinite storage. A mathematical heat transfer model was developed to predict freezing times (phase change was considered) required for bull semen and extender packaged in 0.5ml plastic straws and suspended in static liquid nitrogen vapor. Thermophysical properties (i.e. thermal conductivity, specific heat, density, initial freezing temperature) of bovine semen and extender as a function of temperature were determined considering the water change of phase. The non-stationary heat transfer partial differential equations with variable properties (nonlinear mathematical problem) were numerically solved considering in series thermal resistances (semen suspension-straw) and the temperature profiles were obtained for both semen suspension and plastic straw. It was observed both the external heat transfer coefficient in stagnant nitrogen vapor and its temperature (controlled by the distance from the surface of liquid nitrogen to the straw) affected freezing times. The accuracy of the model to estimate freezing times of the straws was further confirmed by comparing with experimental literature data. Results of this study will be useful to select "safe" holding times of bull semen in plastic straws placed N(2)V/LN(2) to ensure that complete freezing of the sample has occurred in the nitrogen vapor and avoid cryodamage when plunging in LN(2). Freezing times predicted by the numerical model can be applied to optimize freezing protocols of bull semen in straws. PMID:23159803

  15. Characteristics of Antioxidant Systems of Yellow Fraction of Red Deer's (Cervus elaphus L.) Semen During the Rutting Period.

    Science.gov (United States)

    Koziorowska-Gilun, M; Szurnicka, M; Dziekonska, A; Kordan, W; Giżejewski, Z; Filipowicz, K

    2016-04-01

    The objective of this study was to make the preliminary characterization of the antioxidant defence systems of the yellow fraction (YF) of red deer's (Cervus elaphus L.) semen during the rutting period. The semen was collected using artificial vagina (AV). The studies included spectrophotometric determination of antioxidant enzymes activities such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also analysed the contents of low-molecular antioxidants such as L-glutathione (GSH + GSSG), L-ascorbate (ASC) and total antioxidant status (TAS). Additionally, the samples were subjected to PAGE and stained for SOD and GPx activities. It was demonstrated that the yellow fraction exhibited activities of SOD and GPx, with the highest activities in September and October. CAT activity was not detected. Staining for the SOD and GPx activities confirmed three protein bands with SOD activity and one protein band with GPx activity. The content of GSH + GSSG was similar in trials dating from October to December contrary to the content of ASC which was high in samples from September and October. The stable rate of TAS was observed during the whole rutting period. The results of this study showed that the YF of red deer semen is equipped with basic battery of antioxidant enzymes comprising SOD and GPx, with the supporting role of GSH + GSSG and ASC. Moreover, the samples obtained at the peak of the rutting period occurring from September to October had the highest enzymatic activity in comparison with remaining months of the rutting period, which contributed to the high quality of the semen by preventing it from the formation of oxidative stress during the short period of intense sexual activity of male red deer. The better understanding of the mechanisms of antioxidant defence systems in the YF of deer's semen may contribute to the potential use of this fraction in technology of wild ruminant semen preservation. PMID:26854018

  16. Effects of Experimental Fasciola gigantica Infection on Serum Testosterone Profiles in Relationship to Semen Characteristics of Yankasa Ram

    Directory of Open Access Journals (Sweden)

    D. Iliyasu

    2014-10-01

    Full Text Available This study was designed to evaluate the effects of Fasciolosis on serum testosterone profiles and semen characteristics of Yankasa rams. Twelve, apparently healthy, Yankasa rams aged 18-24 months were randomly divided into two groups (infected group A and control group B with seven and five rams, respectively. The rams werekept under intensive management in different pens throughout the study period. Group A rams were inoculated with 800 metacecariae orally and monitored for 12 weeks Post Infection (PI. Clinical signs were manifested 2 weeks PI. Faecal examination revealed Fasciola eggs at week 7 PI in group A. Three mL of blood were collected aseptically via the jugular vein at 30 min interval, weekly between 08:00-9:00am for serum testosterone assay. Testosterone assay was done using ELISA technique. There was significant decrease in testosterone concentration in group A at different time intervals of the weeks of post-infection. The semen analysis showed the lowest mean (±SEM of semen volume, semen motility, semen concentration, of group A, as (0.01±0.0 mL, (3.6±2.4%, (16.1±4.2% compared to group B with mean (±SEM (1.6±0.1 mL, (93.0±1.2%, (440.0±13.0% at week 7 post-infection, respectively. This study revealed that Fasciolosis has a great impact on sheep mainly by diminishing their reproductive efficiency and rendering infected rams infertile. It is concluded that F. gigantica infectionhad advanced effects on testosterone production and semen quality of Yankasa rams.

  17. Effect of varicocele on semen characteristics according to the new 2010 World Health Organization criteria: a systematic review and meta-analysis

    Science.gov (United States)

    Agarwal, Ashok; Sharma, Reecha; Harlev, Avi; Esteves, Sandro C

    2016-01-01

    This study investigated the effects of varicocele on semen parameters in infertile men based on the new 2010 World Health Organization laboratory manual for the examination of human semen. Semen analysis results (volume, sperm count, motility, and morphology) were the primary outcomes. An electronic search to collect the data was conducted using the Medline/PubMed, SJU discover, and Google Scholar databases. We searched articles published from 2010 to August 2015, i.e., after the publication of the 2010 WHO manual. We included only those studies that reported the actual semen parameters of adult infertile men diagnosed with clinical varicocele and contained a control group of either fertile men or normozoospermic men who were not diagnosed with varicocele. Ten studies were included in the meta-analysis, involving 1232 men. Varicocele was associated with reduced sperm count (mean difference: −44.48 × 106 ml−1; 95% CI: −61.45, −27.51 × 106 ml−1; P < 0.001), motility (mean difference: −26.67%; 95% CI: −34.27, −19.08; P < 0.001), and morphology (mean difference: −19.68%; 95% CI: −29.28, −10.07; P < 0.001) but not semen volume (mean difference: −0.23 ml; 95% CI: −0.64, 0.17). Subgroup analyses indicated that the magnitude of effect was influenced by control subtype but not WHO laboratory manual edition used for semen assessment. We conclude that varicocele is a significant risk factor that negatively affects semen quality, but the observed pooled effect size on semen parameters does not seem to be affected by the WHO laboratory manual edition. Given most of the studies published after 2010 still utilized the 1999 manual for semen analysis, further research is required to fully understand the clinical implication of the 2010 WHO laboratory manual on the association between varicocele and semen parameters. PMID:26780872

  18. Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis

    Directory of Open Access Journals (Sweden)

    Kamran Dorostkar

    2012-12-01

    Full Text Available The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis bulls. Five healthy buffalo bulls (5 ejaculates from each bull were used. Each ejaculate was diluted at 37 ?C with tris-based extender containing 0 (control, 0.5, 1, 2, 4 and 8 ?g mL-1 sodium selenite and the sperm motility and viability were evaluated at 0 (T0 (immediately after dilution, 60 (T1 and 120 (T2 min after diluting semen. In the second step, semen samples were diluted with tris-egg yolk-glycerol extender containing the same amounts of sodium selenite, cooled to 4 ?C, equilibrated and semen parameters (motility, viability, membrane integrity and DNA damage were estimated. Then, the semen was packed in 0.5 mL French straws and frozen in liquid nitrogen. Later, the semen was thawed and analyzed for the same parameters, as well as total antioxidant capacity. Results showed that addition of 1 and 2 ?gmL-1 selenium to the semen extender significantly increased the sperm motility of fresh and equilibrated semen compared to the control without affecting other parameters. However, in frozen-thawed semen, extenders containing 1 and 2 ?g mL-1 selenium significantly improved sperm motility, viability, membrane integrity and semen total antioxidant capacity and also resulted in lower DNA damaged sperms. In this study selenium supplementation of semen extender of 4 and 8 ?g mL-1 had deleterious effects on sperm parameters as early as the samples were prepared for freezing.

  19. Evaluation of the semen swim-up method for bovine sperm RNA extraction.

    Science.gov (United States)

    Han, C M; Chen, R; Li, T; Chen, X L; Zheng, Y F; Ma, M T; Gao, Q H

    2016-01-01

    Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the E.Z.N.A.(TM) Total RNA kit II, with non-swim-up sperm as a control. Total sperm RNA was analyzed by UV spectrophotometry, reverse transcription polymerase chain reaction (RT-PCR), and agarose gel electrophoresis, and expression of the sex-determining region on the Y chromosome (SRY), leptin (LEP), and ribosomal protein subunit 23 (RPS23) genes, were determined. 18S RNA was used as a positive control. Fewer somatic cells were found in sperm swim-up samples than in the non-swim-up counterparts (0 x 10(3) vs 17.33 ± 2.52 x 10(3) sperm, P RNA was obtained in about 2 h, with no significant difference between groups. Interestingly, the yields of RNA fragments containing ≥200 nucleotides were significantly reduced in sperm swim-up samples (0.92 ± 0.41 x 10(7) sperm) compared with the non-swim-up samples (1.36 ± 0.33 x 10(7) sperm, P RNA were found in RNA samples from the sperm swim-up group. Our findings suggest that small amounts of sperm RNA can be efficiently extracted from frozen straw semen samples using the swim-up technique. PMID:27173315

  20. The effect of recombinant follicle-stimulating hormone on semen parameters after varicocelectomy in infertile men

    Directory of Open Access Journals (Sweden)

    Atoosa Bagheri Behzad

    2015-12-01

    Full Text Available Background: Infertility is defined as failure to achieve pregnancy after one year of unprotected sexual intercourse. Infertility can be related to male or female factors. Varicocele is the most common cause of infertility in men that is correctable with surgery. The purpose of this study was to determine the effects of recombinant follicle-stimulating hormone (rFSH on semen parameters in infertile men. Methods: This randomized clinical trial was done on 96 infertile men admitted to the Women's General Hospital Mohebe-Yas from September 2014 to September 2015. Inclusion criteria were to include varicocelectomy for unilateral idiopathic varicoceles and consent to participate in the study. Allergy to the drug combination and patient dissatisfaction were exclusion criteria. Patients participating in the study were divided into two groups randomly, one group received recombinant FSH three times a week and the other group received a placebo (normal saline in the same way. After three months, the improvement of semen parameters, including motility, morphology and sperm count as well as the complications were determined in both groups. The data were analyzed with statistical software SPSS version 13 (Chicago, IL, USA. Results: A total of 96 patients were enrolled in two groups of 48 men and women; both groups were matched in terms of underlying factors. The rate of improvement in the morphology and motility of sperm in the treated group was significantly more than the placebo group (P= 0.0001; but the changes in sperm count were not significantly different between the groups (P= 0.495. Conclusion: In summary, based on the results obtained in this study, it can be concluded that recombinant FSH is effective on improving semen parameters in infertile men after varicocelectomy compared with a placebo group and its major impact is on the morphology and motility of sperm.