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Uranium quantification in semen by inductively coupled plasma mass spectrometry.  

Science.gov (United States)

In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2g aliquots of pooled control semen at three different levels of uranium: low at 5 pg/g, medium at 50 pg/g, and high at 1000 pg/g. The detection limit was determined to be 0.8 pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9 pg/g of semen (n=10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (semen is consistent with known differences in current DU body burdens in these individuals, some of whom have retained embedded DU fragments. PMID:22944582

Todorov, Todor I; Ejnik, John W; Guandalini, Gustavo; Xu, Hanna; Hoover, Dennis; Anderson, Larry; Squibb, Katherine; McDiarmid, Melissa A; Centeno, Jose A

2013-01-01

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Efecto de la adición de plasma seminal en el semen equino descongelado / Effect of seminal plasma addition on frozen-thawed equine semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Antecedentes y objetivos: El semen criopreservado ofrece beneficios adicionales no presentes en el semen refrigerado. Sin embargo, varios factores afectan al éxito en la inseminación artificial con semen congelado de caballos. El objetivo del trabajo es evaluar si la adición de plasma seminal a dife [...] rentes concentraciones, sobre espermatozoides equinos descongelados, afecta a la motilidad espermática, viabilidad y a nivel de membrana. Material y métodos: Se utilizaron diferentes razas, cuatro sementales de silla, y dos sementales de tiro. En un primer experimento el semen descongelado se centrifugó, mientras en el segundo no se centrifugó. A continuación, se adicionó el plasma seminal al 10, 20, 30% suspendido en solución tampón fosfato y plasma seminal puro (100%). Resultados: En los caballos de silla el plasma seminal no afectó a los parámetros estudiados (p>0,05), pero se apreció un posible efecto tóxico del plasma seminal puro sobre las características espermáticas. En las muestras con plasma seminal de los caballos de tiro, se observaron unos índices mejores en espermatozoides vivos con acrosoma intacto que en las muestras control. Asimismo se obtuvo un porcentaje menor en espermatozoides reaccionados que en las muestras control, encontrando en esta categoría una diferencia significativa (p Abstract in english Background and objectives: Stallion sperm cryopreservation offers benefits not available in cooled semen. However various factors affect the success of artificial insemination with frozen-thawed equine semen. This study aims to evaluate if adding different concentrations of seminal plasma on frozen- [...] thawed equine spermatozoa affects sperm motility, viability and membrane status. Material and Methods: Different breeds were used; four saddle stallions and two draft stallions. In the first experiment thawed semen was centrifuged and in the second one it was not. Subsequent to that, the spermatozoa resuspended with 10, 20, 30% seminal plasma in phosphate buffered saline and pure seminal plasma (100%). Results: semen parameters of saddle stallions were not affected (p>0,05), but a possible toxic effect of pure seminal plasma was observed on sperm characteristics. Seminal plasma samples in draft breed got better rates in viable sperm with intact acrosome. A lower percentage was also found on spermatozoa with acrosome reaction than in control samples. This category showed signif icant differences (p

D., Lozano Benito; L., Gil Huerta; C., Álvarez San Martín.

2011-09-01

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Study on the relationship between the trace protein contents in semen plasma and male fertility  

International Nuclear Information System (INIS)

Objective: To explore the relationship between the trace protein contents in semen plasma and male fertility. Methods: The semen plasma concentrations of albumin (Alb), ?2-microglobulin (?2-m), ?2-microglobulin (?2-m), TH glycoprotein (THP), immunoglobulin G (IgG), secreting-type immunoglobulin A (SIgA), and ferritin (Fer) were determined with RIA in 22 fertile and 125 sterile males. Results: With the exception of ferritin, the semen plasma contents of all these trace proteins in the sterile individuals were lower than those in the fertile ones and there were significant differences (p2-m, Alb and Fer were positively correlated to the sperm counts. Contents of SIgA and IgG could reflect the local immune status of the genital tract. Determination of the contents of these trace proteins in semen plasma would be helpful in the evaluation and management of male infertility

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Lead level in seminal plasma may affect semen quality for men without occupational exposure to lead  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Infertility affects approximately 10–15% of reproductive-age couples. Poor semen quality contributes to about 25% of infertile cases. Resulting from the direct effect on testicular function or hormonal alterations, heavy metals exposure has been related to impaired semen quality. The objective of this study was to assess the level of lead in the seminal plasma in men without occupational exposure to lead, and to determine the relationship between semen quality and lead concentration in the semen. Methods This is a prospective and nonrandomized clinical study conducted in University infertility clinic and academic research laboratory. Three hundred and forty-one male partners of infertile couples undergoing infertility evaluation and management were recruited to the study. Semen samples collected for the analyses of semen quality were also used for the measurement of lead concentrations. Semen samples were evaluated according to the WHO standards. Results All subjects were married and from infertile couples without occupational exposure to lead. There is a significant inverse correlation between the lead concentration in seminal plasma and sperm count. A higher semen lead concentration was correlated with lower sperm count, but not with semen volume, sperm motility or sperm morphology as assessed by simple linear regression. Conclusions We found that semen lead concentration was significantly higher among the patients with lower sperm count. To our knowledge, this is the first study to demonstrate that a high level of lead accumulation in semen may reduce the sperm count contributing to infertility of men without occupational exposure to lead.

Wu Hsien-Ming

2012-11-01

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Boar seminal plasma components and their relation with semen quality.  

Science.gov (United States)

Select boar seminal plasma (SP) components and their relation to semen quality were investigated. Thirty nine boars from three artificial insemination (AI) centers were divided into group A (GA:?>?80% normal sperm and >70% motility) and group B (GB:?nigrosin staining) were investigated. The SP was separated and analyzed for aspartate-amino-transferase (AST), ?-glutamyl-transferase (GGT), alkaline phosphatase (ALP) activity, and the concentrations of sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphate (PO(4)(3-)), magnesium (Mg), selenium (Se) and zinc (Zn) were assessed. Repeated measures (2 months interval) were conducted in eight boars of GA from one AI center. The activity of GGT (r?=?-0.482) and ALP (r?=?-0.459) was moderately associated (p?semen quality. The analysis of biochemical parameters could provide extra information about reproductive health of AI boars. PMID:23083319

López Rodríguez, Alfonso; Rijsselaere, Tom; Beek, Josine; Vyt, Philip; Van Soom, Ann; Maes, Dominiek

2013-02-01

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Relationship of zinc concentrations in blood and seminal plasma with various semen parameters in infertile subjects  

International Nuclear Information System (INIS)

To find out relationship of zinc concentrations in blood and seminal plasma with various semen parameters between fertile and infertile men. (JPMC), Karachi and Department of Biochemistry. Basic Medical Sciences Institute, JPMC, Karachi. Fifty eight primary infertile male subjects, without any treatment, who had regular unprotected intercourse for at least 12 months without conception with their partners, aged 20-40 years, were selected from Infertility Clinic Jinnah Postgraduate Medical Center, Karachi. After semen analyses they were grouped as, oligospermic (30), and azoospermic (28). Twenty five known fertile male selected from general population and after semen analysis were taken as normospermic control group. Semen analyzed according to WHO criteria. Serum and seminal plasma zinc were estimated by 5Br. PAPS Colorimetric method. This study showed significant difference in serum and seminal zinc levels in normospermic, oligospermic (p<0.05) and azoospermic (p<0.005). Seminal plasma zinc showed a positive correlation with sperm count and negative with sperm motility in normospermic and oligospermic and negative correlation with volume, pH, WBC concentration in all three groups. There was no correlation found with sperm morphology. On the basis of the findings of this study and those of other reports, zinc may contribute to fertility through its significant effects on various semen parameters. It seems that the estimation of seminal plasma zinc may help in investiga seminal plasma zinc may help in investigation and treatment of infertile males. (author)

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Seminal Plasma and Semen Amyloids Enhance Cytomegalovirus Infection in Cell Culture  

Science.gov (United States)

Among the modes of transmission available to the cytomegalovirus (CMV) is sexual transmission, primarily via semen. Both male-to-female (M-F) and male-to-male (M-M) sexual transmission significantly contribute toward the spread of CMV infections in the global population. Semen plays an important role in carrying the viral particle that invades the vaginal or rectal mucosa, thereby initiating viral replication. Both semen and seminal plasma (SP) can enhance HIV-1 infection in cell culture, and two amyloid fibrils, semen-derived enhancer of viral infection (SEVI) and amyloids derived from the semenogelins (SEM amyloids), have been identified as seminal factors sufficient to enhance HIV-1 infection (J. Munch et al., Cell 131:1059–1071, 2007; N. R. Roan et al., Cell Host Microbe 10:541–550, 2011; F. Arnold et al., J. Virol. 86:1244–1249, 2012). Whether SP, SEVI, or SEM amyloids can enhance other viral infections has not been extensively examined. In this study, we found that SP, SEVI, and SEM amyloids strongly enhance both human CMV (HCMV) and murine CMV infection in cell culture. SEVI and SEM amyloids increased infection rates by >10-fold, as determined by both flow cytometry and fluorescence microscopy. Viral replication was increased by 50- to 100-fold. Moreover, viral growth curve assays showed that SP, SEVI, and SEM amyloids sped up the kinetics of CMV replication such that the virus reached its replicative peak more quickly. Finally, we discovered that SEM amyloids and SEVI counteracted the effect of anti-gH in protecting against CMV infection. Collectively, the data suggest that semen enhances CMV infection through interactions between semen amyloid fibrils and viral particles, and these interactions may prevent HCMV from being neutralized by anti-gH antibody. PMID:24027327

Roan, Nadia R.; Yamamura, Yasuhiro

2013-01-01

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Effects of Copper and Superoxide Dismutase Content of Seminal Plasma on Buffalo Semen Characteristics  

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Full Text Available To investigate the effects of copper and superoxide dismutase (SOD content of seminal plasma on buffalo semen characteristics, 54 semen samples collected from buffalo bulls by a bovine artificial vagina were used. Semen characteristics (motility, viability, morphology, concentration and volume were recorded. Seminal plasma was harvested by centrifugation and kept frozen until analysis. Seminal plasma copper content was determined by atomic absorption procedure and SOD was measured by using a kit. The mean total copper value of seminal plasma was recorded as 2.51 ± 0.04 mg kg-1 (Mean ± SEM and the mean total SOD values was 39.02 ± 0.81 IU mL-1. To reduce the range of variability, the data were categorized according to their motility records in 3 groups of Excellent (Ex, >90% motile, n = 33, Good (Go, 80-89% motile, n = 15 and Moderate (Mo, <79% motile, n = 6. The mean motility, viability, copper and SOD values in Ex group was recorded as 92.24 ± 0.51%, 94.00 ± 0.48%, 2.56 ± 0.04 mg kg-1 and 39.52 ± 0.57 IU mL-1, respectively. These values were 81.66 ± 0.62%, 85.26 ± 0.95%, 2.38 ± 0.11 mg kg-1 and 36.48 ± 1.51 IU mL-1 in Go group and 71.66 ± 1.05%, 77.00 ± 2.94%, 2.55 ± 0.10 mg kg-1 and 50.66 ± 2.51 in Mo group, respectively. The mean copper value in Ex group was highly (r = 0.600 correlated with SOD and correlated with sperm motility (r = 0.372 and viability (r = 0.363, while, in Go group it was highly correlated (r = 0.945 with SOD and sperm viability (r = 0.652 and in Mo group it was correlated (r = 0.874 with semen volume only. The mean SOD values in Ex group was highly correlated with sperm motility (r = 0.492 and viability (r = 0.490 and mean copper values, in Go group, it was highly correlated whit sperm viability (r = 0.659 and mean copper values and in Mo group it had no significant correlations with semen parameters. These results suggest that copper and SOD content of the buffalo seminal plasma have an influence on the sperm motility and viability which are the most important factors in semen fertility.

M. Eghbali

2008-01-01

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Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes.  

Science.gov (United States)

In the Canadian Animal Genetic Resource Program, bull semen is donated in frozen or fresh (diluted) states. This study was designed to assess the cryopreservation of diluted bull semen shipped at 4°C overnight, and to determine the post-thaw quality of shipped semen using different straw volumes and freezing rates. Semen was collected from four breeding bulls (three ejaculates per bull). Semen was diluted in Tris-citric acid-egg yolk-glycerol (TEYG) extender, cooled to 4°C and frozen as per routine (control semen). After cooling to 4°C, a part of semen was removed and shipped overnight to the research laboratory via express courier (shipped semen). Semen was packaged in 0.25 or 0.5 ml straws and frozen in a programmable freezer using three freezing rates, i.e., -10, -25 or -40°C/min. Control semen was also shipped to the research laboratory. Post-thaw sperm motility characteristics were assessed using CASA, and post-thaw sperm plasma membrane, mitochondrial membrane potential and normal acrosomes were assessed using flow cytometry. Post-thaw sperm quality was greater in shipped semen as compared to control (Pbanking. Overnight shipping of semen was found advantageous for bull semen cryopreservation. Semen packaging in 0.25 ml straws yielded better post-thaw quality than 0.5 ml straws. PMID:21621352

Anzar, M; Kroetsch, T; Boswall, L

2011-06-01

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Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.  

Science.gov (United States)

Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and physiology, including sperm motility, the objectives of this study were to characterize the chemistry and protein profiles of Asian elephant seminal plasma and to determine the relationships between seminal plasma components and semen quality. Ejaculates exhibiting good sperm motility (?65%) expressed higher percentages of spermatozoa with normal morphology (80.3±13.0 vs. 44.9±30.8%) and positive Spermac staining (51.9±14.5 vs. 7.5±14.4%), in addition to higher total volume (135.1±89.6 vs. 88.8±73.1 ml) and lower sperm concentration (473.0±511.2 vs. 1313.8±764.7×10? cells ml?¹) compared to ejaculates exhibiting poor sperm motility (?10%; P<0.05). Comparison of seminal plasma from ejaculates with good versus poor sperm motility revealed significant differences in concentrations of creatine phosphokinase, alanine aminotransferase, phosphorus, sodium, chloride, magnesium, and glucose. These observations suggest seminal plasma influences semen quality in elephants. One- and two-dimensional (2D) gel electrophoresis revealed largely similar compositional profiles of seminal plasma proteins between good and poor motility ejaculates. However, a protein of ?80 kDa was abundant in 85% of ejaculates with good motility, and was absent in 90% of poor motility ejaculates (P<0.05). We used mass spectrometry to identify this protein as lactotransferrin, and immunoblot analysis to confirm this identification. Together, these findings lay a functional foundation for understanding the contributions of seminal plasma in the regulation of Asian elephant sperm motility, and for improving semen collection and storage in this endangered species. PMID:23976974

Kiso, Wendy K; Selvaraj, Vimal; Nagashima, Jennifer; Asano, Atsushi; Brown, Janine L; Schmitt, Dennis L; Leszyk, John; Travis, Alexander J; Pukazhenthi, Budhan S

2013-01-01

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Primary study on the clinical significance of measurement of epidermal growth factor (EGF) and NPY concentrations in human semen plasma  

International Nuclear Information System (INIS)

Objective: To investigate the difference between the semen plasma contents of EGF and NPY in fertile and non-fertile males with the relevant sperm count and motility. Methods: Semen plasma contents of EGF and NPY were determined with RIA in 110 non-fertile males. Simultaneous semen analysis revealed (1) Group A, n=45, with normal sperm count, (2) Group B, n=34 low sperm count (0-20) x 106/ml and (3) Group C n=31, with aspermia. White blood cell/HPF was examined in all the semen specimens and sperm motile rate and motility were examined in Group A specimens. Results: The semen plasma contents of EGF and NPY in non-fertile males were significantly higher than those in fertile males (P 1 x 106/ml) were significantly lower than those in specimens with more white blood cells (P<0.05). Conclusion: Higher semen plasma contents of EGF and NPY might exert toxic effect on the sperms, contributing to the development of infertility. (authors)

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Clinical significance of determination of semen plasma IL-2, IL-6, IL-8 and TNF-? contents in infertile males  

International Nuclear Information System (INIS)

Objective: To explore the influence of high semen plasma contents of the cytokines (IL-2, IL-6, IL-8, TNF-?) on male fertility. Methods: Semen plasma levels of IL-2, IL-6, IL-8, and TNF-? were determined with RIA in 126 infertile and 20 fertile males. Results: Semen plasma contents of the 4 cytokines in infertile subjects were significantly higher than those in fertile ones (p4/HP, n=15) had significantly higher contents of cytokines than those without leucocytospermia (WBC<4/HP, n=111). Besides, TNF-? contents in subjects with lower sperm activity and less motility rate as well as IL-8 contents in subjects with less sperm motility rate were both significantly higher than those in subjects with more normal sperms (p<0.01, p<0.05). Conclusion: High semen plasma cytokines contents represent existing local infection and enhanced auto-immune status, both damaging to sperms. Infertility would be the inevitable consequence. Monitoring of changes of the cytokine contents should be a part of fertility studies

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Storage of fresh bovine semen in a diluent based on the ionic composition of cauda epididymal plasma.  

Science.gov (United States)

For artificial insemination (AI) in cattle, much lower insemination doses can be applied when fresh semen is used instead of frozen-thawed semen. However, a particular disadvantage of fresh semen is its limited shelf life. As bovine spermatozoa can be stored for several weeks in the cauda epididymis without negative effects on their fertilizing capacity, it is an interesting organ to serve as a model in order to prolong the shelf life of fresh semen. First, the storage capacity of a diluent [cauda epididymal plasma (CEP-1)] with the same ionic composition, pH and osmolarity as the bovine CEP was compared with a Tris diluent for extended preservation of fresh ejaculated bovine semen. Secondly, the ionic composition of the CEP-1 diluent was modified (CEP-2) and its storage capacity was compared with this of the CEP-1 and Tris diluent. Finally, the effect of addition of different polyols (sorbitol, glycerol, mannitol) and egg yolk concentrations (5, 10 and 20%) to the CEP-2 diluent was assessed. Sperm quality decreased rapidly in the CEP-1 diluent. The quality and especially progressive motility of spermatozoa stored in the CEP-2 diluent were better those in the CEP-1 and Tris diluent. No significant effects of different sugars or egg yolk concentrations on the quality of fresh bovine semen in the CEP-2 diluent were observed. In conclusion, the CEP-2 diluent with 10% egg yolk and 1 g/l sorbitol may be used for extended preservation of fresh bovine semen at 5 degrees C up to 6 days. PMID:15598230

Verberckmoes, S; Van Soom, A; Dewulf, J; De Pauw, I; de Kruif, A

2004-12-01

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Effect of holding of semen and washing of seminal plasma on quality and fertility of Hampshire boar semen preserved at liquid state.  

Science.gov (United States)

The present study was aimed to reveal the effect on keeping quality of boar semen on holding or not holding at an elevated temperature than that used for preservation when combined with washing or not washing of seminal plasma. Twenty ejaculates, four from each of five Hampshire boars were used to hold for 0 and 4h in GEPS extender at 22°C and subsequently washed (1500×g for 10min) of seminal plasma or left unwashed and preserved at 15°C for 72h after extending with the same extender. The seminal parameters in terms of sperm motility, live spermatozoa, and live spermatozoa with intact acrosome (LIA) were evaluated at 0h-(immediately after extension) and thereafter at 24h intervals. The mean percentage of sperm motility was significantly (Psemen at both 0h and 4h of holding irrespective of preservation period. It was significantly (Psemen held for 4h than 0h irrespective of washing and significantly (Psemen irrespective of holding during preservation. Irrespective of preservation period the mean percentage of live spermatozoa was significantly (Psemen and was significantly (Psemen at both 0h and 4h of holding. It was significantly (Psemen irrespective of washing and was significantly (Psemen irrespective of holding during preservation. The mean percentage of LIA was significantly (Psemen and was significantly (Psemen at both 0h and 4h of holding irrespective of preservation period. It was significantly (Psemen irrespective of washing and was significantly (Psemen irrespective of holding during preservation. The mean percentage of sperm motility, live spermatozoa and LIA decreased significantly (Psemen irrespective of holding with increase in preservation period. Among all the treatments unwashed semen held for 4h yielded superior sperm quality on preservation. A total of 32 female pigs were inseminated using preserved semen obtained with the best processing technique found in the study. The conception rate, farrowing rate and litter size at birth were recorded to be 81.25%, 78.13% and 7.96 respectively as compared to 73.38%, 67.57% and 6.68 respectively in the control group. It could be concluded that unwashed Hampshire boar semen held for 4h, extended with GEPS and preserved at 15°C for 72h was conducive to obtain optimum fertility and fecundity in females when used for artificial insemination. PMID:24559728

Chutia, T; Biswas, R K; Tamuli, M K; Deka, B C; Sinha, S; Goswami, J; Banik, S; Kayastha, R B

2014-03-01

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Detection of haptoglobin in seminal plasma of Awassi rams and the relation with its level in serum and some semen parameters.  

Science.gov (United States)

The study was conducted to detect haptoglobin in seminal plasma (SP-Hp) of Awassi rams and the effect of the breeding season on its concentration, along with determining the correlation with its concentration in serum (S-Hp) and main semen variables. Pre-warmed artificial vagina was used to collect semen samples biweekly from five Awassi rams. Semen samples were evaluated for volume, concentration and sperm motility. Blood samples were collected 10-30 min after semen collection. The concentration of serum and seminal plasma Hp was determined using ELISA. The mean ± SESP-Hp concentrations ranged from 0.25 ± 0.05 to 0.81 ± 0.44 ?g/ml, whereas those of S-Hp-ranged from 0.99 ± 0.29 to 2.99 ± 0.18 mg/ml. There was a significant (P semen volume and sperm concentration (r = -0.164 and -0.121), whereas sperm concentration positively correlated with individual sperm motility (r = 0.100). No significant correlation was detected between SP-Hp and semen parameters. It can be concluded that, Hp is present in ram seminal plasma and its concentration was about 2000 folds lower than that of the serum, and Hp concentration was lower during the breeding season, but its concentration in seminal plasma has no significant correlation with semen parameters. PMID:25685465

Aziz, Dhafer M; Ahmad, Ahmad K

2013-11-01

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Effects of the Seminal Plasma Iron and Lead Content on Semen Quality of Water Buffalo (Bubalus bubalis Bulls  

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Full Text Available In order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. The semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption spectrophotometer. The iron and lead content of the seminal plasma (Mean ± SEM was recorded as 40.68 ± 0.75 mg L-1 and 0.026 ± 0.008 mg L-1, respectively. The mean iron value was highly associated with sperm progressive motility, gross motility and viability, negatively with lead content, and had a negative association with semen volume. The mean lead value was highly negatively associated with sperm progressive motility, gross motility, viability and positively associated with sperm abnormal morphology.For further clarification of these associations, the results were categorized in three groups of excellent (Ex, > 90 % motile, n = 33, good (Go, 80-89 % motile, n = 15 and moderate (Mo, < 79 % motile, n = 6 according to their percentage of sperm motility. The mean progressive motility in Ex, Go and Mo group was 92.24 ± 0.51 %, 81.66 ± 0.62 %, and 71.66 ± 1.05 % respectively. The mean iron and lead values and their associations with other parameters in these groups are discussed.The results show that seminal plasma iron content is associated with the motility and viability of the spermatozoa after ejaculation, but its lead content has an adverse effect on these parameters.

Mohammad-Hassan Khadem Ansari

2010-12-01

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Influence of dietary zinc on semen traits and seminal plasma antioxidant enzymes and trace minerals of beetal bucks.  

Science.gov (United States)

Zinc (Zn) is a potent antioxidant and plays a key role in scavenging free radicals. We hypothesized that supplementation of Zn would reduce the oxidative damage, which is linked with poor sperm quality. Sixteen bucks of similar average age (2 years) and body weight (41 kg) were randomly divided into four groups viz., 1, 2, 3 and 4 supplemented with zinc sulphate into the diet at the rate of 0, 50, 100 and 200 mg/buck/day, respectively, for 3 months. At the end of the experiment, semen samples were collected and assessed. Seminal plasma was separated to find the concentration of superoxide dismutase (SOD), glutathione peroxidase (GPx), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and trace minerals (Zn, Cu, Mn and Fe). The results revealed that semen volume (1.85 ± 0.01 ml) and sperm motility (88.23 ± 5.77%) increased significantly (p 0.05) was observed. From the present results, we concluded that zinc sulphate at the rate of 100 mg/buck/day improved semen traits and seminal plasma antioxidant capacity in Beetal bucks. PMID:25263460

Rahman, H U; Qureshi, M S; Khan, R U

2014-12-01

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Detection of haptoglobin in seminal plasma of Awassi rams and the relation with its level in serum and some semen parameters  

Directory of Open Access Journals (Sweden)

Full Text Available The study was conducted to detect haptoglobin in seminal plasma (SP-Hp of Awassi rams and the effect of the breeding season on its concentration, along with determining the correlation with its concentration in serum (S-Hp and main semen variables. Pre-warmed artificial vagina was used to collect semen samples biweekly from five Awassi rams. Semen samples were evaluated for volume, concentration and sperm motility. Blood samples were collected 10–30 min after semen collection. The concentration of serum and seminal plasma Hp was determined using ELISA. The mean ± SESP-Hp concentrations ranged from 0.25 ± 0.05 to 0.81 ± 0.44 ?g/ml, whereas those of S-Hp-ranged from 0.99 ± 0.29 to 2.99 ± 0.18 mg/ml. There was a significant (P < 0.05 positive correlation (r = 0.329 between SP-Hp and S-Hp concentrations. Both SP-Hp and S-Hp concentrations were significantly (P < 0.05 higher in winter as compared with the other seasons. The concentrations of SP-Hp and S-Hp during the breeding season were significantly lower (P < 0.01 than those of the out season period. SP-Hp concentration negatively correlated with semen volume and sperm concentration (r = ?0.164 and ?0.121, whereas sperm concentration positively correlated with individual sperm motility (r = 0.100. No significant correlation was detected between SP-Hp and semen parameters. It can be concluded that, Hp is present in ram seminal plasma and its concentration was about 2000 folds lower than that of the serum, and Hp concentration was lower during the breeding season, but its concentration in seminal plasma has no significant correlation with semen parameters.

Dhafer M. Aziz

2013-11-01

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Effects of the Seminal Plasma Zinc Content and Catalase Activity on the Semen Quality of Water Buffalo (Bubalus bubalis Bulls  

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Full Text Available In order to determine zinc and catalase content of seminal plasma in the buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen volume and sperm concentration, gross and progressive motility and viability were evaluated, seminal plasma was then harvested by centrifugation and its zinc content was estimated by atomic absorption spectrophotometer and its catalase activity determined by using a commercial kit. The zinc content of the seminal plasma (Mean ± SEM was recorded as 154.40 ± 1.74 mg L-1, while, the mean catalase value was 32.00 ± 0.42 U mL-1. The mean zinc values was highly correlated with sperm progressive motility and viability and with catalase values (p = 0.000 for all and also was associated with gross motility (p = 0.020 and negatively with abnormal morphology (p = 0.049. The catalase values were highly associated with sperm progressive motility, viability and zinc content (p = 0.000 for all and was associated with sperm gross motility (p = 0.024. For further clarification of these correlations, the samples were categorized in three groups of excellent (Ex, > 90% motile, n = 33, good (Go, 80-89% motile, n = 15 and moderate (Mo, < 79% motile, n = 6 according to their percentage of sperm motility. The mean progressive motility in Ex group was 92.54 ± 0.51%, in Go group was 81.66 ± 0.62% and in Mo group was 71.66 ± 1.05%. The mean zinc and catalase values were recorded as 161.07 ± 1.63 mg L-1 and 33.41 ± 0.34 U mL-1 in Ex, 146.70 ± 1.91 mg L-1 and 31.01 ± 0.67 in Go and 136.42 ± 4.97 mg L-1 and 26.51 ± 0.87 U mL-1 in Mo groups. The mean zinc value in Ex group was highly associated with sperm motility, viability and catalase values, in Go group was associated with catalase values and highly associated with sperm abnormal morphology and in Mo group it was highly associations with catalase values only. The mean catalase value in Ex group, was highly associated with sperm motility and viability, in Go group was associated with zinc content and in Mo groups was highly associated with the zinc content. These results show that seminal plasma zinc and catalase content are correlated with semen characteristics and synergistically act to preserve motility and viability of the spermatozoa after ejaculation.

S.M. Alavi-Shoushtari

2009-01-01

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The relationship between seminal plasma aspartate aminotransferase activity, sperm osmotic resistance test value, and semen quality in boars  

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Full Text Available The relationship between the activity of aspartate aminotransferase (AspAT in seminal plasma and the values of the osmotic resistance test (ORT of acrosomal membranes and semen traits was examined on 120 young hybrid Pietrain and Duroc boars. The following semen quality traits were determined: the volume of the ejaculate, the percentage of spermatozoa with progressive motility, sperm concentration and the total number of spermatozoa in the ejaculate, percentage of spermatozoa with normal acrosome, the percentage of spermatozoa with major and minor morphological defects, ORT, and the activity of AspAT in seminal plasma. The activity of AspAT in seminal plasma was negatively correlated (p_0.01 with the spermatozoa concentration and total number per ejaculate, percentage of spermatozoa with progressive motility and percentage of spermatozoa with a normal acrosome, while positively with the percentage of spermatozoa with major (p?0.001 and minor (p?0.01 morphological defects. The ORT values negatively correlated with the percentage of spermatozoa with major (p?0.05 and minor (p?0.01 morphological defects, while positively (p?0.001 with the percentage of spermatozoa with a normal acrosome.

Jacyno Eugenia

2013-01-01

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Sperm survival kinetics in different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation.  

Science.gov (United States)

This study was designed to compare the kinetics of sperm survival in different types of bull semen. Fresh ejaculates from four bulls were pooled, diluted in Tris-citric acid-egg yolk-glycerol extender, cooled to 4°C, frozen in LN2 and thawed at 37°C. Fresh, diluted, cooled and frozen-thawed semen were incubated at 37°C, and evaluated at 0, 2, 4, 6, 12 and 24h after the beginning of incubation. In Experiment 1, progressive sperm motility, normal acrosomes and plasma membrane integrity and asymmetry were determined. In Experiment 2, generation of superoxide anion (O2•) along with plasma membrane permeability and generation of hydrogen peroxide (H2O2) along with plasma membrane integrity were assessed. In Experiment 1, frozen-thawed semen had shorter survival times for progressive sperm motility, and spermatozoa with intact plasma membranes and acrosomes (IPM-IACR) as compared with other types of semen (P2•- generation and increased plasma membrane permeability, and became necrotic without H2O2 generation during incubation (P2•-, which increased the permeability and compromised the integrity of the plasma membrane. PMID:24576435

Ahmad, Mushtaq; Ahmad, Nasim; Riaz, Amjad; Anzar, Muhammad

2014-02-28

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Radio-neuroprotective effect of L-alpha-glycerylphosphorylcholine (GPC) in an experimental rat model.  

Science.gov (United States)

Ionizing radiation plays a major role in the treatment of brain tumors, but side-effects may restrict the efficacy of therapy. In the present study, our goals were to establish whether the administration of L-alpha-glycerylphosphorylcholine (GPC) can moderate or prevent any of the irradiation-induced functional and morphological changes in a rodent model of hippocampus irradiation. Anesthetized adult (6-weeks-old) male Sprague-Dawley rats were subjected to 40 Gy irradiation of one hemisphere of the brain, without or with GPC treatment (50 mg/kg bw by gavage), the GPC treatment continuing for 4 months. The effects of this partial rat brain irradiation on the spatial orientation and learning ability of the rats were assessed with the repeated Morris water maze (MWM) test. Histopathologic (HP) evaluation based on hematoxylin-eosin and Luxol blue staining was performed 4 months after irradiation. The 40 Gy irradiation resulted in a moderate neurological deficit at the levels of both cognitive function and morphology 4 months after the irradiation. The MWM test proved to be a highly sensitive tool for the detection of neurofunctional impairment. The site navigation of the rats was impaired by the irradiation, but the GPC treatment markedly decreased the cognitive impairment. HP examination revealed lesser amounts of macrophage density, reactive gliosis, calcification and extent of demyelination in the GPC-treated group. GPC treatment led to significant protection against the cognitive decline and cellular damage, evoked by focal brain irradiation at 40 Gy dose level. Our study warrants further research on the protective or mitigating effects of GPC on radiation injuries. PMID:24880750

Plangár, Imola; Szabó, Emília Rita; T?kés, Tünde; Mán, Imola; Brinyiczki, Kitti; Fekete, Gábor; Németh, István; Ghyczy, Miklós; Boros, Mihály; Hideghéty, Katalin

2014-09-01

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Proteínas do plasma seminal de caprinos relacionadas com o índice pluviométrico e a qualidade do sêmen / Proteins of goat seminal plasma related with precipitation index and semen quality  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O objetivo deste estudo foi identificar as proteínas do plasma seminal de caprinos da raça Alpina Americana criados na região Nordeste do Brasil que estão relacionadas ao índice pluviométrico e à qualidade do sêmen. O sêmen foi obtido pelo método de vagina artificial a partir de três reprodutores e [...] foi avaliado quanto aos parâmetros macroscópicos e microscópicos. O perfil de proteínas do plasma seminal foi realizado por eletroforese bidimensional. Os parâmetros volume do sêmen, integridade do acrossoma e proteínas totais evidenciaram diferença significativa (P Abstract in english The aim of this study was to identify proteins in seminal plasma of goats raised in the Northeast of Brazil related with precipitation index and semen quality. Semen was obtained from three bucks and evaluated to the microscopic and macroscopic parameters. The profile of seminal plasma proteins was [...] performed by analysis of two-dimensional electrophoresis. Volume, acrosome integrity and total proteins had significant difference (P

Andreia Fernandes de, Souza; Maria da Conceição Gomes, Leitão; André Mariano, Batista; Ana Lúcia Figueiredo, Porto; José Luiz de, Lima Filho; Maria Madalena Pessoa, Guerra.

1155-11-01

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Ram seminal plasma improves pregnancy rates in ewes cervically inseminated with ram semen stored at 5 °C for 24 hours.  

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In this study, we compared pregnancy rates obtained using ram semen stored at 5 °C for 24 h, with ram or bull seminal plasma (SP) added to TRIS-egg yolk extender. During the breeding period, 670 adult Corriedale ewes were cervically inseminated with semen (2 × 10(8) sperm in a volume of 0.2 mL) from eight adult Corriedale rams. Ejaculates, obtained using an artificial vagina, were split into three aliquots and diluted with the following: TRIS-egg yolk based extender (T), T + 30% ram SP (R), or T + 30% bull SP (B). Samples were refrigerated and stored at 5 °C for 24 h until used for AI. Pregnancy was assessed by ultrasonography 35 to 40 d after AI. Pregnancy rate was not affected by ram (P = 0.77) or breeding period (P = 0.43), and there were no interactions between extender and ram (P = 0.94), or extender and breeding period (P = 0.24). However, there was an effect of extender (P = 0.0009) on pregnancy rates; ram SP, but not bull SP, increased pregnancy rates compared with extender without SP (49.7, 38.1, and 31.1%, for R, B, and T respectively). In conclusion, ram SP added to TRIS-egg yolk extender had a beneficial effect on the pregnancy rate of ram sperm stored at 5 °C for 24 h and used for cervical insemination of ewes. PMID:21958634

López-Pérez, A; Pérez-Clariget, R

2012-01-15

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Effects of vitamins, probiotics, and protein level on semen traits and some seminal plasma macro- and microminerals of male broiler breeders after zinc-induced molting.  

Science.gov (United States)

This study was conducted to investigate the effects of vitamin E, vitamin C, probiotics, dietary protein level, and their combination on semen traits and seminal plasma macro- and microminerals in 65-week-old male broiler breeders after zinc-induced molting. One hundred eighty birds were induced to molt by mixing zinc oxide (3,000 mg/kg) in the diet. The birds were divided into six groups (five replicates) by completely randomized design. One group was kept as control (16% CP), while the other five were supplemented with vitamin E (100 IU/kg feed), vitamin C (500 IU/kg feed) probiotics (50 mg/L), protein level (14% CP), and their combination. Semen samples were weekly collected for determination of semen volume, sperm concentration, motility, and dead sperm percentage. Analyses of Na, K, Ca and Mg, Zn, Fe, Mn, and Cu in seminal plasma were also performed. Overall, mean semen volume was significantly high in vitamin E and C supplemented groups compared to control. Overall mean sperm motility was significantly higher in vitamin E supplemented group, whereas dead sperm percentage was significantly lower in the vitamin C group compared to control. Mineral analyses revealed that overall mean seminal plasma Mg increased significantly in vitamin E and C supplemented groups compared to control. Similarly, significantly high overall mean seminal plasma Cu concentration was observed in vitamins E and C and combination groups. It can be concluded that vitamins have a vital role in improving semen quality and bioavailability of Mg and Cu in seminal plasma of the post-molt cockerels. PMID:22318702

Khan, Rifat Ullah; Zia-Ur-Rahman; Javed, Ijaz; Muhammad, Faqir

2012-07-01

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Calcium, Magnesium and Total Antioxidant Capacity (TAC in Seminal Plasma of Water Buffalo (Bubalus Bubalis Bulls and their Relationships with Semen Characteristics  

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Full Text Available In order to determine calcium (Ca, magnesium (Mg content and total antioxidant capacity (TAC of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its Ca and Mg content were estimated and its TAC determined. The Ca and Mg content of the seminal plasma (Mean ± SEM were recorded as 22.36 ± 0.52 mg dl-1 and 11.94 ± 0.36 mg dl-1 respectively, while, its mean TAC value was 1.50 ± 0.02 mmol L-1. The mean Ca value was highly associated with sperm progressive motility, gross motility, viability (P = 0.000 for all, negatively with semen volume (P = 0.01, and with Mg and TAC values (P = 0.000 for both. The mean Mg values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and TAC (P = 0.000 for all and negatively associated with semen volume (P = 0.014. The mean TAC values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and Mg (P = 0.000 for all. For further clarification of these associations, the data was categorized in three groups of excellent (Ex, >90% motile, n = 33, good (Go, 80-89% motile, n = 15 and moderate (Mo, <79% motile, n = 6 according to their percentage of sperm motility. The mean progressive motility in Ex group was 92.24 ± 0.51%, in Go group it was 81.66 ± 0.62 %, and in Mo group it was 71.66 ± 1.05 %. The mean Ca, Mg and TAC values were respectively recorded as 25.12 ± 0.29 mg dl-1, 13.78 ± 0.20 mg dl-1, and 1.57 ± 0.009 mmol L-1 in Ex, 18.74 ± 0.63 mg dl-1, 9.14 ± 0.33mg dl-1, and 1.42 ± 0.044 mmol L-1 in Go, and 17.34 ± 0.18 mg dl-1, 8.06 ± 0.25 mg dl-1, and 1.23± 0.05 mmol L-1 in Mo groups. The associations in groups are discussed. These results show that seminal plasma Ca and Mg content and TAC are associated with semen characteristics, and synergistically have an effect on motility and viability of the spermatozoa after ejaculation, which are important factors in semen fertility.

Mohammad-Hassan Khadem Ansari

2010-06-01

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Effect of extenders on sperm mitochondrial membrane, plasma membrane and sperm kinetics during liquid storage of canine semen at 5°C.  

Science.gov (United States)

The objective of this study was to evaluate the effect of addition of egg yolk (EY) or soy lecithin (SL) based extenders on dog sperm parameters during 10days storage at 5°C. Four ejaculates of pooled semen from three Mongrel dogs were divided into three aliquots and extended to a final concentration of 200 million sperm/mL using following non-commercial extenders: egg yolk extender (EYE) group - semen+20% Tris-EYE; soy lecithin (SL) group 1 - semen+SL extender with 0.04% SL; SL group 2 - semen+SL extender 0.4% SL. The extended semen samples were stored at 5°C and were evaluated for sperm mitochondrial membrane potential (MMP), plasma membrane integrity (%PMI) and computer assisted sperm analyzer (CASA) sperm motility parameters on days 0, 1, 2, 3, 4, 7 and 10. The extender, days of storage and extender×days of storage affected the MMP, %PMI and CASA sperm motility parameters (P<0.05). The addition of 0.4% SL extender reduced the speed of deterioration of sperm parameters evaluated in this study compared to EY and 0.04% SL extenders (P<0.05). In conclusion, the addition of 0.4% SL extender enhanced sperm preservation based on the variables evaluated in the present study compared to EYE and 0.04% SL extenders and plausibly preserves sperm quality longer. PMID:23182466

Kasimanickam, Vanmathy R; Kasimanickam, Ramanathan K; Memon, Mushtaq A; Rogers, Harmon A

2012-12-01

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Selección Espermática en Semen Congelado/Descongelado de Equino: Evaluación de las Membranas Plasmática, Acrosomal y Potencial de Membrana Mitocondrial / Sperm Selection in Frozen/Thawed Semen of Equine: Evaluation of Plasma, Acrosome Membranes and Mitochondrial Membrane Potential  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Los procedimientos de criopreservación inducen cambios morfofuncionales en los espermatozoides. Es importante post descongelación espermática utilizar procedimientos de selección que permitan recuperar espermatozoides altamente funcionales. El objetivo del presente estudio fue comparar la eficiencia [...] del Swim-up y Equipure® en la selección de espermatozoides funcionales en semen descongelado de equino. Semen de 4 potros reproductores Criollos Chilenos (A, B, C y D), fueron descongelados separadamente y procesados (n=15) por: I.- Swim-up (SU) y II.- Equipure® (EQ). Post descongelación se determinó por citometría de flujo la viabilidad e integridad de membrana plasmática (SYBR-14/PI), potencial de membrana mitocondrial (YDm; JC-1), integridad de la membrana acrosomal (FITC-PSA/PI). La motilidad progresiva (%) en dos animales fue más alta (P Abstract in english Freeze-thaw procedures induce structural and functional changes in sperm. It is important to use post thaw sperm selection procedures that can retrieve highly functional sperm. The aim of this study was to compare the efficiency of the Swim-up and Equipure® in the selection of functional sperm of th [...] awed equine semen. Semen of four Chilean Criollo reproductive stallions (A, B , C and D) were frozen and thawed using a standard protocol and processed separately (n = 15) : I. Swim-up (SU) and II. Equipure® (EQ). Post sperm selection,was determined by flow cytometry. Viability and plasma membrane integrity (SYRB-14/PI), mitochondrial membrane potential (YDm, JC -1), acrosome membrane integrity (FITC-PSA/PI). Progressive motility (%) was higher (P

Paulina, Cabrera; Raúl, Sánchez; Jennie, Risopatrón.

2014-06-01

29

Effect of the addition of seminal plasma, vitamin E and incubation time on post-thawed sperm viability in boar semen / Efecto de la adición de plasma seminal, vitamina E y tiempo de incubación en la viabilidad post-congelamiento del esperma en semen de verraco  

Scientific Electronic Library Online (English)

Full Text Available SciELO Mexico | Language: English Abstract in spanish El objetivo del estudio fue evaluar el efecto del plasma seminal (PS), Vitamina E (VE) y tiempo de incubación sobre la viabilidad espermática de semen de verracos después de su congelamiento. Treinta y seis eyaculados fueron usados y asignados a cuatro tratamientos: Tl, semen + BTS (Solución de post [...] congelamiento Belstville) + 10% PS; T2, semen + BTS + 200?g/ml VE; T3, semen + BTS + 10% PS + 200ug/ml VE; T4, semen + BTS (control). La motilidad (MOT), integridad de acrosomas (IA), integridad de membrana (IM) y la actividad mitocondrial (AM) se evaluaron a los 0 y 30 min después del congelamiento. Se utilizó un diseño en parcelas divididas y los datos se analizaron mediante un análisis de varianza para modelos mixtos. Se encontró efecto significativo de PS y VE sobre IA y IM (PO.05) pero no sobre MOT y AM (P>0.05). Hubo efecto significativo de tiempo de incubación sobre MOT (21.3 y 27.9%) y IA (46.0 y 36.0%), a los 0 y 30 min postcongelación (P Abstract in english The objective of the study was to evaluate the effect of seminal plasma (SP), vitamin E (VE), and incubation time on sperm viability of post-thawed boar semen. Thirty six ejaculates were used and allocated to four treatments: Tl, semen + BTS (Belstville Thawing Solution) + 10% SP; T2, semen + BTS + [...] 200?g/ml VE; T3, semen + BTS + 10% SP + 200ug/ml VE; T4, semen + BTS (control). Motility (MOT), intact acrosomes (IA), membrane integrity (MI) and mitochondrial activity (MA) were evaluated, at 0 and 30 min after thawing. A split plot design was used and the data analyzed using a mixed model analysis of variance. There was a significant effect of SP and VE on IA and MI (PO.05) but not on MOT and MA (P>0.05). There was significant effect of incubation time on MOT (21.3 and 27.9%) and IA (46.0 and 36.0%), at 0 and 30 min post-thawing (P

A. G. C., Pech- Sansores; F. G., Centurión- Castro; J. C., Rodríguez-Buenfil; J. C., Segura-Correa; J. R., Aké-Lopez.

2011-12-01

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Alergia al semen / Semen allergy  

Scientific Electronic Library Online (English)

Full Text Available La alergia al semen comprende una variedad de síntomas tanto locales como sistémicos causados por reacciones de hipersensibilidad inmediata y caracterizados por títulos elevados de IgE. El objetivo de este estudio es describir el caso de una paciente con alergia al semen: mujer de 21 años de edad qu [...] e presenta ardor y sensación de quemazón en el área genital luego de tener contacto con el semen de su pareja. El análisis seminal del compañero sexual no presenta ningún tipo de alteración. Los síntomas desaparecen con el uso de condón o con la práctica del coito interrumpido. La alergia al semen es una alteración, que si bien es poco frecuente, puede afectar los deseos de concepción de las mujeres que la presentan, es un fenómeno poco estudiado por lo que se requieren más reportes para su caracterización. Abstract in english Semen allergy includes several local and systemic symptoms caused by immediate hypersensitivity reactions and it is characterized by high levels of IgE. The objective of this study was to describe the case of a patient with semen allergy. A 21 year-old woman experienced itching and burning sensation [...] in the genital area after contact with the semen of her sexual partner. Semen analysis was normal. Symptoms disappear with the use of condom or the practice of coitus interruptus. Semen allergy is a condition, although rare, can affect the desire of conceiving in women who suffers it. It is a briefly studied phenomenon which requires more reports for proper characterization.

Laura, Franco Cuadros; Jenniffer, Puerta Suárez; Ángela, Cadavid Jaramillo; Walter, Cardona Maya.

2013-12-01

31

Semen Analysis  

Science.gov (United States)

... Bookmark this Page Email this Page Home › Semen analysis is a test on the fluid that is released when a man has an ... | SART Web Site Terms & Conditions of Use | Web Design and Development by The Berndt Group

32

Effect of vitamins, probiotics and protein level on semen traits and seminal plasma biochemical parameters of post-moult male broiler breeders.  

Science.gov (United States)

1. A study was designed to investigate the comparative effect of supplementary vitamin E, vitamin C, probiotics and dietary crude protein concentration on semen traits and seminal plasma biochemical parameters in male broiler breeders after Zn-induced moulting. 2. A total of 180 male broiler breeders were induced to moult at 65 weeks of age by mixing ZnO in diet at the rate 3000?mg/kg of feed. After moulting, the males were divided into six groups that were given feed supplemented with: vitamin C (500?IU/kg), vitamin E (100?IU/kg), protein (140?g CP/kg), probiotics (50?mg/kg) and the combination of these components, while one group was kept as a control. Semen samples were collected weekly and semen volume, spermatozoa concentration, motility and dead spermatozoa percentage were determined. Seminal plasma was separated to determine the concentration of total antioxidant capacity (TAC), homocysteine, paraoxonase (PON1), arylesterase, ceruloplasmin, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities. 3. Semen volume was significantly higher in the vitamin E and C groups compared to the control. Spermatozoa motility was higher in the vitamin E group and dead spermatozoa percentage was significantly lower in the vitamin C group compared with the control group. 4. Seminal plasma TAC was higher in the vitamin E group, homocysteine was lower in the vitamin C and E groups. PON1 was higher in the combination group. Arylesterase increased significantly in the vitamin C and combination groups over time. Seminal plasma AST was significantly lower in the vitamin C and E supplemented groups whereas ALT decreased significantly only in the vitamin E group compared with the control. Higher concentrations of ceruloplasmin were observed in the combination group compared with the other treatments. 5. It was concluded that additional vitamin E and C or their combination was the most potent nutrient treatment for improving the semen traits and seminal plasma biochemical characteristics in male broiler breeders after Zn-induced moulting. PMID:23444861

Khan, R U; Rahman, Z U; Javed, I; Muhammad, F

2013-01-01

33

Effect of heterologous seminal plasma and semen extenders on motility of frozen-thawed ram spermatozoa  

OpenAIRE

Ram seminal plasma increases the fertility of frozen-thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP, TrilEq (Triladyl with 0.5 m? of Equex STM paste added to each 100 m?) and heat-treated skim milk on longevity and percentages of progressively motile and aberrantly motile frozen-thawed ram spermatozoa. Three ejaculates from each of 6 rams w...

Gerber, D.; Nothling, J. O.; Terblanche, S. J.; Mataveia, G. A.

2012-01-01

34

Effect of heterologous seminal plasma and semen extenders on motility of frozen-thawed ram spermatozoa  

Directory of Open Access Journals (Sweden)

Full Text Available Ram seminal plasma increases the fertility of frozen-thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP, TrilEq (Triladyl with 0.5 m? of Equex STM paste added to each 100 m? and heat-treated skim milk on longevity and percentages of progressively motile and aberrantly motile frozen-thawed ram spermatozoa. Three ejaculates from each of 6 rams were extended in TrilEq, pooled and frozen in straws as a single batch per ram. One hundred and eight straws (3 straws from each ram for each fluid were thawed in random order. Once thawed, a straw was emptied into a tube with 0.85m? of the appropriate fluid at 37 °C and kept at that temperature for 6 h. Motility was assessed at x200 magnification immediately (time zero and 2, 4 and 6 h after thawing. Progressive motility decreased from each time to the next (P < 0.05 and was 39.0% (0 h, 26.0% (2 h, 19.6% (4 h and 12.6% (6 h; SEM 1.24, n=108 for each group. Ram seminal plasma resulted in higher progressive motility than bull seminal plasma, lower than milk, and similar to the other fluids. Ram seminal plasma resulted in lower aberrant motility than protein-free TALP and similar aberrant motility to other fluids. The effect of ram seminal plasma and dog prostatic fluid was very similar. The effect of ram seminal plasma on the fertility of frozen-thawed ram spermatozoa deposited into the cervix is not due an exceptionally beneficial effect on the motility of spermatozoa.

D. Gerber

2012-05-01

35

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality  

OpenAIRE

Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and physiology, including sperm motility, the objectives of this study were to characterize the chemistry and protein profiles of Asian elephant seminal plasma and to determine the relationships betwee...

Kiso, Wendy K.; Selvaraj, Vimal; Nagashima, Jennifer; Asano, Atsushi; Brown, Janine L.; Schmitt, Dennis L.; Leszyk, John; Travis, Alexander J.; Pukazhenthi, Budhan S.

2013-01-01

36

Comparative biochemistry of mammalian epididymal plasma.  

Science.gov (United States)

1. A comparison has been made of the chemical composition of epididymal plasma from the cauda epididymidis of nine mammalian species. 2. Results have shown that epididymal plasma contains low concentrations of inorganic ions and high levels of several unusual organic constituents, among which may be mentioned glycerylphosphorylcholine, hypotaurine, carnitine and several glycosidases and phosphatases. 3. The influence of this milieu on the motility and survival of spermatozoa is discussed. PMID:318382

Jones, R

1978-01-01

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Immunoreactive luteinizing hormone-releasing hormone in the seminal plasma and human semen parameters  

International Nuclear Information System (INIS)

A luteinizing hormone-releasing hormone (LH-RH)-like substance has been detected in human seminal plasma by a radioimmunoassay (RIA) with a highly specific anti-LH-RH antiserum. The seminal samples - not only the plasma itself but also the sample extracted by an acid/alcohol method - showed satisfactory displacement curves in our RIA system. The relationship between fertility and the LH-RH values in the seminal plasma was studied by comparing the peptide levels with sperm concentration and motility. By these two parameters, 103 samples were divided into four groups. In the low-concentration groups (oligozoospermic patients), the hormonal concentrations differed significantly between those specimens demonstrating good and poor motility. These data suggest that this immunoreactive LH-RH may play a role in human spermatogenesis

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Seminal plasma concentrations of Anti-Müllerian hormone and inhibin B predict motile sperm recovery from cryopreserved semen in asthenozoospermic men: a prospective cohort study.  

Science.gov (United States)

The rate of motile sperm recovery after cryopreservation is very variable and difficult to predict. Anti-Müllerian hormone (AMH) and inhibin B are produced by Sertoli cells and released into the seminal plasma, where they could be functional markers of spermatogenesis and sperm resistance to thermal stress. The aim of this study was to evaluate whether seminal plasma levels of AMH and inhibin B predict sperm recovery after cryopreservation. The study included 153 men enrolled prospectively during a semen analysis. The cohort was stratified by the fresh semen characteristics into: normal (n = 52), high sperm count (n = 55), asthenozoospermia (n = 23), and oligozoospermia (n = 23). The main outcome measure was motile sperm recovery rate, defined as post-thaw total motile sperm count × 100/pre-freezing total motile sperm count. In men with asthenozoospermia there was a significant correlation between motile sperm recovery rate and the pre-freezing concentrations of AMH (r = 0.522, p characteristic curves of AMH and inhibin B for prediction of ?50% motile sperm recovery after cryopreservation were, respectively, 0.808 and 0.638. AMH was particularly useful, with sensitivity of 0.85, specificity of 0.80, positive predictive value of 0.84 and negative predictive value of 0.80. The sensitivity, specificity, positive, and negative predictive values of inhibin B for the same outcome were, respectively, 0.62, 0.60, 0.67, and 0.55. The median motile sperm recovery rate was 83% when seminal plasma AMH concentration was ?0.84 ng/mL, vs. 27% when AMH concentration was semen cryopreservation in asthenozoospermic men. PMID:25269872

Nery, S F; Vieira, M A F; Dela Cruz, C; Lobach, V N M; Del Puerto, H L; Torres, P B; Rocha, A L L; Reis, A B; Reis, F M

2014-11-01

39

Effect of heterologous seminal plasma and semen extenders on motility of frozen-thawed ram spermatozoa  

Scientific Electronic Library Online (English)

Full Text Available SciELO South Africa | Language: English Abstract in english Ram seminal plasma increases the fertility of frozen-thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP, TrilEq (Triladyl with 0.5 m? of Equex STM paste [...] added to each 100 m?) and heat-treated skim milk on longevity and percentages of progressively motile and aberrantly motile frozen-thawed ram spermatozoa. Three ejaculates from each of 6 rams were extended in TrilEq, pooled and frozen in straws as a single batch per ram. One hundred and eight straws (3 straws from each ram for each fluid) were thawed in random order. Once thawed, a straw was emptied into a tube with 0.85 m? of the appropriate fluid at 37 ºC and kept at that temperature for 6 h. Motility was assessed at ×200 magnification immediately (time zero) and 2, 4 and 6 h after thawing. Progressive motility decreased from each time to the next (P

G A, Mataveia; S J, Terblanche; J O, Nöthling; D, Gerber.

2010-09-01

40

The activity of N-acetyl-?-hexosaminidase in boar seminal plasma is linked with semen quality and its suitability for cryopreservation.  

Science.gov (United States)

The determination of sperm cryotolerance is an important step in the process of developing optimal techniques for the storage of boar semen. The objective of this study was to determine individual proteome variations in boar seminal plasma and spermatozoa and establish their influence on the cryotolerance of ejaculate. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed the presence of protein with estimated molecular weight of 90 kDa in sperm extracts from ejaculates of selected boars. In all cases, dialysis performed at the initial stage of cryopreservation effectively removed the protein from sperm cells. The protein had an affinity for Zn(2+) ions. Mass spectrometry revealed similarities between the discussed protein and the ? subunit of N-acetyl-?-hexosaminidase (?-HEX). Seminal plasma ?-HEX was purified 252-fold with approximately 27% recovery and specific activity of 1800 U/mg of protein. Enzyme activity in fresh seminal plasma was correlated with superoxide dismutase activity (r = -0.42, P 20,000 U/L) levels of ?-HEX activity in seminal plasma. In plasma with high ?-HEX activity, spermatozoa were characterized by lower plasma membrane integrity (84.7%, P < 0.05). Higher glutathione levels (1250.3 ?M), higher total protein content (50 mg/mL), and higher total oxidant status (6.82-?mol H2O2 Equiv/L) were also observed (P < 0.05). After thawing, lower sperm motility (20.4%), lower plasma membrane integrity (41.7%), and higher lipid peroxidation (30.9-nM malondialdehyde/10(8) spermatozoa/h) were reported in ejaculates with high seminal plasma ?-HEX activity. The results of this study indicate that ?-HEX activity in seminal plasma is a useful indicator in preliminary evaluations of boar sperm cryotolerance. PMID:25661485

Wysocki, Pawe?; Orzo?ek, Aleksandra; Strze?ek, Jerzy; Koziorowska-Gilun, Magdalena; Zasiadczyk, ?ukasz; Kordan, W?adys?aw

2015-04-15

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INAA determination of selenium via sup(77m)Se in plasma, semen and hair samples from beef and dairy bulls  

International Nuclear Information System (INIS)

Interest in the element selenium with respect to its biological significance has been steadily increasing for the last ten years. Neutron activation analysis has long been used for the accurate determination of selenium in biological samples usually via 75Se. More recently activation analysts having access to high flux reactors with rapid delivery pneumatic tube facilities; have successfully employed sup(77m)Se. This approach, which is much faster, is particularly well suited to the Missouri University Research Reactor (MURR). The specific interest concerning bulls has to do with the involvement of selenium in the reproductive system. Selenium analysis methodology and data on plasma, semen and 22 tissues from both beef and dairy bulls are presented. (author)

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Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo Addition of seminal plasma to frozen-thawed semen and pregnancy rate of fixed time inseminated ewes  

Directory of Open Access Journals (Sweden)

Full Text Available Avaliou-se o efeito da adição de plasma seminal ovino ao sêmen descongelado sobre a taxa de prenhez de ovelhas em rebanho comercial. Cento e setenta e quatro ovelhas cruza Texel foram distribuídas em quatro tratamentos: T1 inseminação artificial cervical (IAC com sêmen descongelado (SD diluído em solução tampão fosfato salino (PBS; T2 IAC com SD e adição de plasma seminal ovino; T3 grupo-controle I: IAC com sêmen fresco diluído em PBS; T4 grupo-controle II: inseminação artificial por laparoscopia com SD diluído em PBS. Para indução de cio, utilizaram-se esponjas impregnadas com acetato de medroxiprogesterona (MAP por 12 dias, com aplicação intramuscular de 400 UI de eCG (Novormon® e de 37,5µg de cloprostenol sódico (Sincrocio®, no dia da retirada das esponjas. O aparecimento de cio foi monitorado com rufiões vasectomizados a partir da retirada das esponjas até a inseminação artificial em tempo fixo - 54 a 60 horas. A taxa de prenhez do tratamento com adição de plasma seminal ao sêmen descongelado (7,0% não diferiu (P>0,05 do tratamento sem adição de plasma (4,3%, entretanto foi menor (PThe effect of seminal plasma addition to thawed-frozen ram semen on the pregnancy rate of commercial herd ewes was evaluated. One hundred and seventy-four crossbred Texel sheep were allocated to four treatments: T1 cervical artificial insemination (CAI using frozen-thawed semen (FTS diluted in phosphate buffered saline solution (PBS; T2 CAI using FTS diluted in ovine seminal plasma; T3 control group I: CAI using fresh semen diluted in PBS; T4 control group II: laparoscopic insemination using FTS diluted in PBS. Estrus induction was performed with medroxiprogesterone acetate (MAP impregnated sponges for 12 days, followed by intramuscular injection of 400 IU of eCG (Novormon® and 37.5µg of sodium cloprostenol (Sincrocio® on the day of sponge removal. Estrus was monitorated with vasectomized rams, beginning at the time of the sponge removal until the fixed time artificial insemination - 54 to 60 hours. The pregnancy rate of FTS diluted in seminal plasma treatment (7.0% did not differ (P>0.05 for the treatment without addition of seminal plasma (4.3%, however it was lower (P<0.05 when compared to the pregnancy rate of the cervical inseminated control I group with PBS diluted fresh semen (50.0% and laparoscopic inseminated control group II with PBS diluted FTS (39.4%. The cervical artificial insemination with the addition of seminal plasma to frozen-thawed semen did not increase the pregnancy rate at acceptable values to make this biotechnology useful on commercial herds.

O.R. Prado

2013-02-01

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Blood in the semen  

Science.gov (United States)

Semen - bloody; Blood in ejaculation ... Most of the time, blood in the semen is caused by swelling or infection of the prostate or seminal vesicles. The problem may occur after a prostate biopsy . Blood in the ...

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Caracterização de proteínas do plasma seminal e sua relação com parâmetros de qualidade do sêmen criopreservado em ovinos / Characterization of seminal plasma proteins and its relationship with quality parameters of frozen semen in ram  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Os objetivos deste trabalho foram analisar o perfil proteico do plasma seminal ovino e identificar proteínas relacionadas com a congelabilidade do sêmen que possam ser utilizadas como marcadores para essa característica. Foram utilizados os ejaculados de cinco reprodutores, nos quais foram realizada [...] s avaliações espermáticas e dos quais os plasmas seminais obtidos por centrifugação foram submetidos à eletroforese bidimensional em gel de poliacrilamida. Foram identificados 92 spots, considerando todos os animais analisados. A avaliação dos dados obtidos evidenciou variações significativas nos resultados das análises do sêmen dos animais e uma variabilidade no perfil proteico no plasma seminal dos carneiros. As proteínas 03 (7,9kDa; pI 6,35), 23 (13,6kDa; pI 5,01) e 31 (21,4kDa; pI 4,75) se destacaram, por apresentarem maior expressão e relações com as características espermáticas. Sugere-se que mais estudos sejam realizados para verificar se as proteínas 03, 23 e 31 podem ser utilizadas como marcadores da capacidade criopreservadora do sêmen. Abstract in english The objective of this study was to analyze the protein profile of ram seminal plasma and to identify proteins associated with semen freezability, which could be used as marker for predicting this feature. Semen from five rams was used. The sperm analysis was held and the seminal plasma obtained by c [...] entrifugation was submitted to two-dimensional electrophoresis using acrylamide gel. Ninety two spots were identified considering the analyzed animals. The results showed a significant variation among sperm analysis of the animals and variability in the protein profile of the seminal plasma of the rams. The proteins 03 (7.9kDa; pI 6.35), 23 (13.6kDa; pI 5.01) e 31 (21.4kDa; pI 4.75) stood out because they showed higher expression and because of its relationship with the sperm characteristics. It is suggested more studies to verify if proteins 03, 23 and 31 could be used as markers of semen freezability.

Priscilla Pereira, Moura; Maurício Machaim, Franco; Thiago Antônio de Souza Nascimento, Silva; Thales Lima, Rocha; Diogo Ramos, Leal; Pedro Ivo Braga, Passos; Jairo Pereira, Neves.

1154-11-01

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Caracterização de proteínas do plasma seminal e sua relação com parâmetros de qualidade do sêmen criopreservado em ovinos Characterization of seminal plasma proteins and its relationship with quality parameters of frozen semen in ram  

Directory of Open Access Journals (Sweden)

Full Text Available Os objetivos deste trabalho foram analisar o perfil proteico do plasma seminal ovino e identificar proteínas relacionadas com a congelabilidade do sêmen que possam ser utilizadas como marcadores para essa característica. Foram utilizados os ejaculados de cinco reprodutores, nos quais foram realizadas avaliações espermáticas e dos quais os plasmas seminais obtidos por centrifugação foram submetidos à eletroforese bidimensional em gel de poliacrilamida. Foram identificados 92 spots, considerando todos os animais analisados. A avaliação dos dados obtidos evidenciou variações significativas nos resultados das análises do sêmen dos animais e uma variabilidade no perfil proteico no plasma seminal dos carneiros. As proteínas 03 (7,9kDa; pI 6,35, 23 (13,6kDa; pI 5,01 e 31 (21,4kDa; pI 4,75 se destacaram, por apresentarem maior expressão e relações com as características espermáticas. Sugere-se que mais estudos sejam realizados para verificar se as proteínas 03, 23 e 31 podem ser utilizadas como marcadores da capacidade criopreservadora do sêmen.The objective of this study was to analyze the protein profile of ram seminal plasma and to identify proteins associated with semen freezability, which could be used as marker for predicting this feature. Semen from five rams was used. The sperm analysis was held and the seminal plasma obtained by centrifugation was submitted to two-dimensional electrophoresis using acrylamide gel. Ninety two spots were identified considering the analyzed animals. The results showed a significant variation among sperm analysis of the animals and variability in the protein profile of the seminal plasma of the rams. The proteins 03 (7.9kDa; pI 6.35, 23 (13.6kDa; pI 5.01 e 31 (21.4kDa; pI 4.75 stood out because they showed higher expression and because of its relationship with the sperm characteristics. It is suggested more studies to verify if proteins 03, 23 and 31 could be used as markers of semen freezability.

Priscilla Pereira Moura

2010-05-01

46

ALKALINE PHOSPHATASE ACTIVITY AS A MARKER OF DOG SEMEN FREEZABILITY  

Directory of Open Access Journals (Sweden)

Full Text Available The investigation was performed to evaluate the dog semen freezability and itsquality after thawing allowing its use for artificial insemination (AI. On the basis ofsperm motility, concentration and alkaline phosphatase (AP activity in semenplasma it was possible to establish that AP activity corresponds with the basic factorof semen examination. Significant statistical differences occurred between thequality of ejaculates which were qualified or disqualified to deep freezing and AI.These results show that AP activity in raw dog semen plasma can be used as amarker for the dog semen qualification for deep freezing and AI with 95%probability of the prognosis of the results.

KOSINIAK-KAMYSZ K.

2007-01-01

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Value of semen parameters, with special reference to TNF-?, in predicting the quality of boar semen after short-term storage.  

Science.gov (United States)

The aim of this study was to address the question whether changes in boar semen quality after short-term storage could be predicted on the basis of standard semen parameters and TNF-? level determined on the day of semen collection under commercial conditions. Progressive motility showed the highest positive correlation with morphology on day 0 of collection, and progressive motility on day 3 (P semen quality after 3 days of storage (AUC > 0.5; P semen storage (r = 0.495, P semen storage (AUC = 0.94, P semen samples with more than 150 pg/ml of TNF-? in the seminal plasma will retain more than 85% of viable spermatozoa after 3 days of storage. Thus, TNF-? can contribute to predicting the quality of short-term stored semen. PMID:23661389

Mrkun, Janko; Kosec, Marjan; Zrimšek, Petra

2013-06-01

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The effect of season on spermatozoa motility, plasma membrane and acrosome integrity in fresh and frozen-thawed semen from Xinong Saanen bucks.  

Science.gov (United States)

The aim of this study was to evaluate whether the season of ejaculate collection influences seminal quality parameters of pre- and post-freeze-thawing in Xinong Saanen bucks. Ejaculates were collected from eight bucks throughout the four seasons (spring, summer, autumn and winter) in a 12 months' time period, identified in the Northern Hemisphere. Semen samples were evaluated by the combinations of conventional and Computer-Assisted Sperm Analysis (CASA) when fresh and after frozen-thawed, respectively. The results clearly demonstrated that season of ejaculate collection influenced (p < 0.05) fresh semen quality. Highest semen quality was observed during autumn. On the contrary, undesirable indices (significantly lower, p < 0.05) were observed in winter as compared with the other remaining seasons. CASA has clearly shown the influences of seasonal variations on semen motility parameters. Furthermore, season of ejaculate collection was also found to influence sperm freezability. Semen characteristics after frozen-thawed followed a similar pattern with that of fresh ejaculate except in spring. The results revealed that sperm quality was higher (p < 0.01) in summer and autumn than in spring and winter. In conclusion, seasonal variation influences semen quality in Xinong Saanen bucks. In addition to summer and autumn, fresh ejaculates in spring can also be successfully used for AI. Sperm from ejaculates collected during summer and autumn are more suitable for cryopreservation. Hence, it is possible to increase the efficiency of goat breeding by manipulating the seasonal variations of semen quality for immediate AI and/or cryopreservation. PMID:25366190

Wang, W; Luo, J; Sun, S; Xi, L; Gao, Q; Haile, A B; Shi, H; Zhang, W; Shi, H

2015-02-01

49

Semen collection using phantom in dromedary camel.  

Science.gov (United States)

Semen collection is relatively long, unsafe, and tedious procedure in dromedary camel. The innovation of safe, hygienic, and simple approach to collect semen could make great progress in development of AI program in this species. This study investigated two methods of semen collection using phantom and artificial vagina in dromedary camel. Semen was collected using phantom (n = 4 bulls; 26 collections) and artificial vagina (n = 6 bulls; 11 collections) and diluted with INRA96 at the ratio of 1:10. The duration of semen collection, semen parameters, and morphometric features of sperm were evaluated. For specimen collected through phantom and AV, the respected duration of semen collection (411.2 ± 48.19 vs 326 ± 37.05 sec), volume (6.6 ± 0.87 vs 6 ± 1.57 ml), osmolarity (328 ± 1.6 vs 319.4 ± 3.21 mOsm/kg H2O), pH (7.7 ± 0.06 vs 7.9 ± 0.16) of semen, concentration (161.4 ± 44.05 × 10(6)/mL vs 160.2 ± 58.42 × 10(6)/mL), total motility (84.1 ± 1.89 vs 78.3 ± 3.97%), progressive forward motility (45.5 ± 3.69 vs 44.3 ± 6.41%), live percentage (72.2 ± 3.11 vs 76 ± 2.53%), and plasma membrane integrity (61.5 ± 2.49 vs 58.9 ± 4.19%) of sperm were similar (P > 0.05). The number of specimens contaminated with visible particles was greater using AV (72.7%) compared to phantom (0%; P < 0.05). Total length, head, middle-piece, and tail length of sperm were 45.9 ± 0.1, 5.6 ± 0.01, 7 ± 0.02, and 34.2 ± 0.16 ?m, respectively. The frequency of abnormal sperm was 13.28% among which coiled tail, detached head, and proximal protoplasmic droplets had greater incidence. In conclusion, phantom could be considered as a suitable approach to collect semen due to simplicity, safety, and lack of specimen contamination in dromedary camel. PMID:25449548

Ziapour, S; Niasari-Naslaji, A; Mirtavousi, M; Keshavarz, M; Kalantari, A; Adel, H

2014-12-10

50

Reproduction in nondomestic birds: Physiology, semen collection, artificial insemination and cryopreservation  

Science.gov (United States)

Pioneering work by Quinn and Burrows in the late 1930s led to successful artificial insemination (AI) programs in the domestic poultry industry. A variety of species specific modifications to the Quinn and Burrows massage technique made AI possible in nondomestic birds. Massage semen collection and insemination techniques span the entire range of species from sparrows to ostriches. Also, cooperative semen collection and electroejaculation have found limited use in some nondomestic species. Artificial insemination produces good fertility, often exceeding fertility levels in naturally copulating populations. However, aviculturists should explore other ways to improve fertility before resorting to AI. Artificial insemination is labor intensive and may pose risks to nondomestic birds as well as handlers associated with capture and insemination. Semen collection and AI makes semen cryopreservation and germ plasma preservation possible. Yet, semen cryopreservation techniques need improvement before fertility with frozen-thawed semen will equal fertility from AI with fresh semen.

Gee, G.F.; Bertschinger, H.; Donoghue, A.M.; Blanco, J.; Soley, J.

2004-01-01

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Superoxide dismutase: a predicting factor for boar semen characteristics for short-term preservation.  

Science.gov (United States)

Superoxide dismutase (SOD), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS) in seminal plasma were evaluated on the basis of receiver operating characteristics (ROC) analysis as predictors for distinguishing satisfactory from unsatisfactory boar semen samples after storage. SOD on day 0 correlated significantly with progressive motility (r=-0.686; PSemen samples that, after 3 days of storage, fulfilled all criteria for semen characteristics (viability>85%, motility>70%, progressive motility>25%, and normal morphology>50%) had significantly lower SOD levels on the day 0 than those with at least one criterion not fulfilled (Psemen will suit the requirements for satisfactory semen characteristics after storage, while semen with SOD levels higher than 1.05?U/mL will not fulfill with 100% accuracy at least one semen characteristic after storage. These results support the proposal that SOD in fresh boar semen can be used as a predictor of semen quality after storage. PMID:24729963

Zakošek Pipan, Maja; Mrkun, Janko; Kosec, Marjan; Nemec Svete, Alenka; Zrimšek, Petra

2014-01-01

52

Multicenter assessment of HIV-1 RNA quantitation in semen in the CREAThE network.  

Science.gov (United States)

Detection of HIV-1 RNA in semen is used commonly to determine the safety of semen processing procedures before assisted reproductive technology (ART). Using two panels of prepared semen samples containing HIV-1 the performances of protocols from 14 centers have been compared. No false-positive results were detected but false-negative results were frequent when the concentration was below 500 HIV-1 RNA copies/ml of seminal plasma. Frequency of HIV-1 RNA detection was higher on seminal cells than on seminal plasma. Assays (or protocols) for quantifying HIV-1 RNA in semen performed less well than standardized blood plasma assays. The HIV load in seminal plasma could be a useful marker of the risk of sexual transmission of the virus. Its use as a marker of global HAART efficiency in the HIV reservoir needs further study. Standardized assays are required for detection and measurement of HIV-1 RNA in semen samples. PMID:22170535

Pasquier, Christophe; Andreutti, Corinne; Bertrand, Evelyne; Bostan, Alionka; Bourlet, Thomas; Molina, Irene; Grossman, Zehava; Halfon, Philippe; Leruez-Ville, Marianne; Lüneborg-Nielsen, Margrethe; Mar, Carmen; Marcelin, Anne-Geneviève; Roussel-Ronserail, Catherine; Schmitt, Marie-Paule; Tabrizi, Sepehr; Vourliotis, Maria; Bujan, Louis

2012-02-01

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Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen.  

Science.gov (United States)

The aim of the present study was to determine the influence of chicken semen cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activities. Pooled semen from 10 Black Minorca roosters was used in the study. Semen samples were subjected to cryopreservation using the "pellet" method and dimethylacetamide (DMA) as a cryoprotectant. In the fresh and the frozen-thawed semen sperm membrane integrity (SYBR-14/propidium iodide (PI)), acrosomal damage (PNA-Alexa Fluor(®)488) and mitochondrial activity (Rhodamine 123) were assessed using flow cytometry. Malondialdehyde (MDA) concentration, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in sperm cells and seminal plasma by spectrophotometry. All sperm characteristics evaluated using flow cytometry were affected by cryopreservation. After freezing-thawing, there was significant (P semen. In seminal plasma of frozen-thawed semen the significant increase (P semen. The present study showed that the activity of CAT, GPx and SOD in chicken semen was affected by cryopreservation, what increased the intensity of lipid peroxidation (LPO). Catalase appeared to play an important role in the sperm antioxidant defense strategy at cryopreservation since, opposite to SOD and GPx, its content was clearly reduced by the cryopreservation process. Change in the antioxidant defense status of the chicken spermatozoa and surrounding seminal plasma might affect the semen quality and sperm fertilizing ability. PMID:22225691

Partyka, Agnieszka; ?ukaszewicz, Ewa; Ni?a?ski, Wojciech

2012-05-01

54

The utility of nanowater for ram semen cryopreservation.  

Science.gov (United States)

Nanowater (NW; water declusterized in the low-temperature plasma reactor) has specific physicochemical properties that could increase semen viability after freezing and hence fertility after artificial insemination (AI) procedures. The main goal of this study was to evaluate ram semen quality after freezing in the media containing NW. Ejaculates from 10 rams were divided into two equal parts, diluted in a commercially available semen extender (Triladyl®; MiniTüb GmbH, Tiefenbach, Germany) prepared with deionized water (DW) or NW, and then frozen in liquid nitrogen. Semen samples were examined for sperm motility and morphology using the sperm class analyzer system and light microscopy. Cryo-scanning electron microscopy (cryo-SEM) was employed to determine the size of extracellular water crystals in frozen semen samples. Survival time at room temperature, aspartate aminotransferase (AspAT) and alkaline phosphatase (ALP) concentrations post-thawing as well as conception/lambing rates after laparoscopic intrauterine AI of 120 ewes were also determined. There were no significant differences between DW and NW groups in sperm progressive motility (26.4?±?12.2 and 30.8?±?12.4%) or survival time (266.6?±?61.3 and 270.9?±?76.7?min) after thawing and no differences in the percentages of spermatozoa with various morphological defects before or after freezing. There were, however, differences (P?semen samples post-thawing. Extracellular water crystals were larger (P?semen (58.3%), and the percentages of ewes that carried lambs to term were 73.3% and 45.0% in NW and DW groups, respectively (P?semen extender prepared with NW was associated with a substantial improvement in the fertilizing ability of frozen-thawed ram semen and lamb productivity of inseminated ewes. PMID:25491414

Murawski, Maciej; Schwarz, Tomasz; Grygier, Joanna; Patkowski, Krzysztof; Oszcz?da, Zdzis?aw; Jelkin, Igor; Kosiek, Anna; Gruszecki, Tomasz M; Szymanowska, Anna; Skrzypek, Tomasz; Zieba, Dorota A; Bartlewski, Pawel M

2014-12-01

55

Between male variation in semen characteristics and preliminary results on the dilution of semen in the ostrich  

Scientific Electronic Library Online (English)

Full Text Available This study is part of an ongoing project on artificial insemination in ostriches. The physical output of neat semen from four ostrich males was investigated and the effect of reconstituting semen with: 1) seminal plasma of the same male (SPS); 2) seminal plasma of another male (SPD), and 3) Dulbecco [...] 's Modified Eagles Medium (DMEM). Semen was collected daily from one or two pairs of males using the dummy female method, each pair being replicated twice. Spermatozoa viability in neat semen, SPS, SPD and DMEM was assessed using nigrosin-eosin staining and the proportions of live normal, live abnormal and dead sperm were determined. Semen volume (mean ± SE) was 1.27 ± 0.13 mL, the concentration of spermatozoa 3.68 ± 0.17 x 10(9) /mL and the number of spermatozoa 4.92 ± 0.64 x 10(9) /ejaculate. Furthermore, the live normal, live abnormal and dead spermatozoa in the neat semen were 61.2 ± 4.5%, 21.2 ± 2.7% and 17.7 ± 4.3% respectively. The ejaculate volume and the number of dead spermatozoa were not affected by collection time. However, the number of live abnormal spermatozoa increased through the day causing a reduction in live normal spermatozoa. Furthermore, re-suspending spermatozoa in DMEM reduced the number of live normal (31.4 ± 4.6%) and live abnormal spermatozoa (11.0 ± 2.7%) and increased the number of dead spermatozoa (57.6 ± 4.4%). In contrast, numbers of live spermatozoa were higher when suspended in seminal plasma and similar in SPS (53.9 ± 4.6%) and SPD (50.7 ± 4.6%). These are the first crucial steps to determining the optimum semen collection time and to improving the viability of diluted spermatozoa.

M., Bonato; P.K., Rybnik; I.A., Malecki; C.K., Cornwallis; S.W.P., Cloete.

56

Relationship of semen hyperviscosity with IL-6, TNF-?, IL-10 and ROS production in seminal plasma of infertile patients with prostatitis and prostato-vesiculitis.  

Science.gov (United States)

Changes in levels of oxidative damage products in semen and their relationship to seminal fluid viscosity (SFV) have recently received increasing research interest. We analysed whether SFV was associated with ROS generation, levels of cytokines TNF-alpha (TNF-?), IL-6 and IL-10 and seminal leucocyte concentration, and whether ROS production was related to the extent of infections/inflammations at one (prostatitis) or two (prostato-vesiculitis) male accessory glands. We studied 169 infertile patients, with chronic bacterial prostatitis (PR, n = 74) and/or bilateral prostato-vesiculitis (PV, n = 95), as diagnosed by the ultrasound (US) criteria. Healthy fertile men (n = 42) served as controls. In the PV patient group, SFV, semen characteristics and ROS production had median values that were significantly higher than those found in PR patients and controls, although other sperm variables had values significantly lower than those found in PR patients or controls. In PV infertile patients, ROS generation and pro-inflammatory cytokines levels were higher than those found in PR infertile patients and controls, although seminal IL-10 levels in PV and PR patients were lower than those found in the controls. In PR patients, the levels of SFV were positively related to TNF-? (r = 0.67; P < 0.01), fMLP-stimulated ROS production in the 45% Percoll fraction (r = 0.687, P < 0.01) and the 90% Percoll fraction in basal condition (r = 0.695, P < 0.01), and after fMLP-stimulation (r = 0.688, P < 0.01). Thus, our data indicated that seminal hyperviscosity is associated with increased oxidative stress in infertile men and increased pro-inflammatory interleukins in patients with male accessory gland infection, more when the infection was extended to the seminal vesicles. PMID:24329571

Castiglione, R; Salemi, M; Vicari, L O; Vicari, E

2014-12-01

57

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen  

Directory of Open Access Journals (Sweden)

Full Text Available The use of several types of gelling extenders for the storage of semen from several domestic species in the solid state has been shown to have beneficial effects on some semen quality parameters. The objective of this study was to evaluate the effect of a new high-viscosity semen extender, Zoosperm ND-5 3D® (Import-Vet, Centelles, Spain, on the the quality of boar spermatozoa at preserved at 17ºC for 7 days. Sodium alginate was used for the first time to increase the viscosity of the extender for the liquid storage of boar semen. The same extender, but without increased viscosity, was used as a control extender (Zoosperm ND-5®, Import-Vet, Centelles, Spain. Sixteen ejaculates from four Pietrain boars were evaluated for motility (by the CASA system, and for viability, acrosome status, plasma membrane fluidity, externalization of phosphatidylserine at the plasma membrane of the spermatozoa and mitochondrial membrane potential (by flow cytometry. In samples diluted with the Zoosperm ND-5 3D® viscous extender, the STR (straightness parameter and the number of progressively motile spermatozoa were higher compared to those of the non-viscous extender (p < 0.05. In addition, the number of spermatozoa with damaged acrosomes, an unstable sperm plasma membrane and externalization of phosphatidylserine at the plasma membrane was lower in samples treated with the viscous extender (p < 0.05. In conclusion, an increase in extender viscosity improves quality of boar spermatozoa following long-term storage.

Maria Cruz Gil

2014-02-01

58

Preservation of honey bee semen.  

Science.gov (United States)

Fertilized eggs have been obtained from queen honey bees (Apis mellifera L.) inseminated with sperm that had been stored in vitro at above-freezing temperatures for up to 68 days. The effects of various experimental storage treatments on semen are described. Semen shipped by ordinary mail has been successfully used for artificial insemination. PMID:13836523

TABER, S; BLUM, M S

1960-06-10

59

Disposition of aspirin and its metabolites in the semen of man.  

Science.gov (United States)

The study was undertaken to determine the distribution of aspirin and its metabolites in the semen of humans after an oral dose of aspirin. Each of seven healthy male volunteers was given a single oral dose of 975 mg of aspirin on an empty stomach together with 200 mL of water. Timed samples of blood and semen were obtained from each subject, and the concentrations of aspirin, salicylic acid, and salicyluric acid determined by a specific high-performance liquid chromatographic assay. The mean peak concentration of aspirin was 6.5 micrograms/mL in plasma (range, 4.9-8.9 micrograms/mL), reached in 26 minutes (range, 13-33 minutes). The half-life of aspirin was 31 minutes. The concentration ratio of aspirin (semen/plasma) was 0.12 (except for one subject in whom it was 0.025). The mean peak concentration of salicylate in plasma was 49 micrograms/mL (range, 42-62 micrograms/mL), reached in 2.5 hours (range, 2.0-2.8 hours). Salicylate distributed rapidly into semen and maintained a concentration ratio (semen/plasma) of 0.15. Salicyluric acid (the glycine conjugate of salicylic acid) was found in the semen. Its high concentration in some subjects' semen (four times the concurrent plasma concentration) was attributed to contamination of semen sample with residual urine, containing salicylurate, in the urethra of those who urinated after the dose of aspirin. Possible side effects of aspirin and salicylate in semen include adverse effects on fertility, male-medicated teratogenesis, dominant lethal mutations, and hypersensitivity reactions in the recipients. PMID:3680588

Kershaw, R A; Mays, D C; Bianchine, J R; Gerber, N

1987-04-01

60

Hepatitis C virus detection in the semen of infected patients  

Scientific Electronic Library Online (English)

Full Text Available Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal p [...] lasma, leukocytes and spermatozoa) were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche) and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%), blood transfusion in four (28.6%), and no risk factors in four (28.6%) patients. Genotype 1 was detected in 62% of the patients, followed by genotype 3 in 23% and genotype 2 in 15%. All blood samples were positive, regardless of the technique used for detection. All semen samples identified by Roche Amplicor and analyzed by agarose gel electrophoresis were negative. Among the 52 semen samples (total and fractions) identified by the Roche Amplicor method, 45 (87%) were inhibited. A negative result was recorded for one (1.9%) total semen sample, one (1.9%) leukocyte and four (7.7%) seminal plasma fractions. Only one (1.9%) sample of the spermatozoon fraction was positive. The results obtained suggested false-negative reactions for the semen samples.

Norma de Paula, Cavalheiro; Ana Carolina de Oliveira, Santos; Carlos Eduardo, Melo; Suzana Rie, Morimitsu; Antonio Alci, Barone.

2008-10-01

61

Detection of bovine papillomavirus type 2 DNA in commercial frozen semen of bulls (Bos taurus).  

Science.gov (United States)

Papillomaviruses are found in epithelial lesions and are linked to different carcinogenic processes in humans and other animals. Although bovine papillomavirus (BPV) has been characterized as epitheliotropic, the presence of viral DNA has been detected in other sample types, including fresh semen. The aim of this study was to evaluate the presence of BPV DNA in spermatozoa and seminal plasma samples of commercial frozen semen taken from bulls (Bos taurus) and its effects on semen function. PCR assays were conducted with specific primers to detect BPV types 1-6 in 40 semen samples of dairy Gir bulls. The semen quality was assessed by the use of parameters such as motility, vigor, acrosomal integrity and DNA integrity. BPV-2 DNA was detected in all of the sperm cell samples and all the seminal samples; however BPV-1, 3, 4, 5 and 6 could not be detected. The presence of BPV DNA was apparently not a cause of reduced sperm function. This is the first record of BPV-2 DNA the commercial frozen semen taken from dairy Gir cattle by several companies that provide semen. Further studies are needed to assess the viability of the virus and the extent to which it can be spread through semen. PMID:22172321

Silva, M A R; Pontes, N E; Da Silva, K M G; Guerra, M M P; Freitas, A C

2011-12-01

62

Advances in boar semen cryopreservation.  

Science.gov (United States)

The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI had been promoted. Recent advances in our laboratory, accommodating the best use of portions of the sperm-rich fraction of the ejaculate for cryopreservation of the sperm-peak portion (P1) and parallel use of the rest of the collected ejaculated spermatozoa, appears as a suitable commercial alternative. PMID:20871820

Rodriguez-Martinez, Heriberto; Wallgren, Margareta

2010-01-01

63

Dark-coloured semen in nonobstructive azoospermia: a report of four cases.  

Science.gov (United States)

Dark-coloured semen is rarely observed in men with nonobstructive azoospermia in absence of genital tract bleeding. Dark-coloured semen is also observed rarely in men with spinal cord injury with or without genital tract bleeding. However, this condition has not been reported in the literature in absence of genital tract bleeding or spinal cord injury. Here, we report men with nonobstructive azoospermia with dark-coloured semen and without genital tract bleeding or spinal cord injury. The study included four subjects with dark-coloured semen. All the samples first investigated for the presence of red blood cells as well as haemoglobin. All the cases were examined in details with seminal, hormonal and genetic parameters. In addition, an elemental electron microscopy and inductively coupled plasma-atomic emission spectrometry evaluation for heavy metals was performed. The semen parameters showed normal volume, pH, leucocyte count and azoospermia or oligoazoospermia. Dark-coloured semen specimens contained neither red blood cells nor haeme pigment. Electron microscopy showed presence of platinum in all the cases, whereas inductively coupled plasma-atomic emission spectrometry revealed increased levels of lead, manganese and nickel in serum as compared to controls. The results indicate dark-coloured semen may be linked with heavy metals found in seminal cells. PMID:23445433

Halder, A; Jain, M; Chaudhary, I; Kumar, G; Das, T; Gupta, Y K

2014-04-01

64

TRIHALOMETHANE LEVELS AND SEMEN QUALITY  

Science.gov (United States)

Trihalomethanes (THMs) are common byproducts of chlorinating drinking water. The effects of disinfection byproducts on semen quality have not yet been studied in humans, despite animal studies linking exposure to sperm abnormalities. We are currently analyzing the relationship of...

65

Concentration, activity and biochemical characterization of myeloperoxidase in fresh and post-thaw equine semen and their implication on freezability.  

Science.gov (United States)

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western blot. Purified active MPO was added in saline solution and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal-Wallis test, and correlations were determined using Spearman's test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post-thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = -0.5576, p = 0.0086). Inactive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post-thaw loss of activity is partially explained by MPO inactivation in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor. PMID:24479950

Ponthier, J; Franck, T; Parrilla-Hernandez, S; Niesten, A; de la Rebiere, G; Serteyn, D; Deleuze, S

2014-04-01

66

Effect of dietary parsley (Petroselinium crispum supplementation on semen quality of local Iraqi ganders  

Directory of Open Access Journals (Sweden)

Full Text Available This study was carried out to investigate the effect of dietary supplementation with different levels of parsley on semen quality of local Iraqi ganders. A total of thirty two local ganders were used in this study during the period from beginning of February to the end of April. The ganders were allocated for 4 treatment groups containing 8 ganders each. Treatment groups were as follows: Control diet (free from parsley, T1: Control diet + 80 g/d parsley, T2: Control diet + 160 g/d parsley; T3: Control diet + 240 g/d parsley. Semen samples were collected twice a week fortnightly from each gander by dorsal-abdominal message method. First semen collection was used to evaluate semen volume, sperm concentration, live in total sperm, live and normal morphology sperm, semen quality factor, sperm motility, abnormal sperm, acrosomal abnormalities, spermatocrit and pH of semen. However, the second semen collection was used for determine seminal plasma concentrations of glucose, protein, cholesterol & activities of aspartate aminotransferase (AST, alanine aminotransferase (ALT and alkaline phosphatase (ALP enzymes. Results revealed that feeding diets containing different levels of parsley (T1, T2, and T3 resulted in significant (P<0.05 increase in semen volume, sperm concentration, live and normal morphology sperm, semen quality factor, sperm motility, spermatocrit and seminal plasma activity of ALP enzyme and significant (P< 0.05 decrease in abnormal sperm and acrosomal abnormalities and seminal plasma concentrations of glucose, protein, and cholesterol and seminal plasma activities of AST and ALT enzymes as compared with control group. There was no significant difference in pH of semen among the control and experimental groups (C, T1, T2, and T3. In conclusion, dietary supplementation with different levels of parsley especially at the level of 240 g/d (T3 caused significant improve- ment with relation to semen traits. So, parsley can be used as an effective tool for improve semen quality of ganders.

Hazim J. Al-Daraji,

2011-08-01

67

Avian artificial insemination and semen preservation  

Science.gov (United States)

Summary: Artificial insemination is a practical propagation tool that has been successful with a variety of birds. Cooperative, massage, and electroejaculation and modifications of these three basic methods of semen collection are described for a variety of birds. Semen color and consistency and sperm number, moti!ity, and morphology, as discussed, are useful indicators of semen quality, but the most reliable test of semen quality is the production of fertile eggs. Successful cryogenic preservation of avian semen with DMSO or glycerol as the cryoprotectant has been possible. Although the methods for preservation require special equipment, use of frozen semen requires only simple insemination supplies

Gee, G.F.

1983-01-01

68

Lipid peroxidation and antioxidant enzymes activity in avian semen.  

Science.gov (United States)

The present study compared the antioxidant system and lipid peroxidation in semen of two avian species: chicken and goose. The experiment was conducted on Greenleg Partridge roosters and White Koluda(®) ganders, each represented by 10 mature males. Malondialdehyde (MDA) concentration, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in sperm cells and seminal plasma. In gander spermatozoa, the amount of MDA was 10 times greater (Prooster spermatozoa. Each of the investigated antioxidant enzymes had greater (P<0.01) activity in goose than chicken sperm. Catalase activity was detected in seminal plasma and spermatozoa from both studied species for the first time. In seminal plasma, the activity of GPx was two times greater (P<0.01) in the White Koluda(®) than in chickens, whereas SOD activity was less (P<0.01) than in chickens. This is the first study describing the presence of CAT in avian semen and the occurrence of indicator of lipid peroxidation (LPO) in geese. Data from the present study clearly show the species-specific differences in the activity of antioxidant defense and LPO. The greater amount of lipid peroxidation and greater activity of antioxidant enzymes in goose semen might suggest that spermatozoa were under greater oxidative stress and the enzymes were not utilized for the protection of functionally and structurally impaired cells. In turn, in fresh chicken semen a lesser activity of antioxidant enzymes accompanied with a lesser lipid peroxidation amount and good semen quality could indicate that fowl spermatozoa were under oxidative stress, but the enzymes were employed to protect and maintain sperm quality. PMID:22884394

Partyka, Agnieszka; Lukaszewicz, Ewa; Ni?a?ski, Wojciech

2012-10-01

69

Associations between dietary patterns and semen quality in men undergoing IVF/ICSI treatment  

OpenAIRE

BACKGROUND: This study investigates whether dietary patterns, substantiated by biomarkers, are associated with semen quality. METHODS: In 161 men of subfertile couples undergoing in vitro fertilization treatment in a tertiary referral clinic in Rotterdam, the Netherlands, we assessed nutrient intakes and performed principal component factor analysis to identify dietary patterns. Total homocysteine (tHcy), folate, vitamin B12 and B6 were measured in blood and seminal plasma. Semen quality wa...

Vujkovic, M.; Vries, J. H. M.; Dohle, G. R.; Bonsel, G. J.; Lindemans, J.; Macklon, N. S.; Spek, P. J.; Steegers, E. A. P.; Steegers-theunissen, R. P. M.

2009-01-01

70

ULTRASTRUCTURAL AND ANNEXIN V-DETECTED DAMAGES IN RAM FROZEN-THAWED SEMEN  

OpenAIRE

Semen cryopreservation and thawing processes may cause damage to sperm membrane structures, which can leads to lower viability and motility of post-thaw sperm. The aim of this study was to examine ultastructural alterations and membrane destabilization in frozen-thawed ram semen. Ultrastructural alterations were detected using transmission electron microscopy (TEM). Analyzed sperm heads were classified into 4 categories: I - sperm with intact plasma membrane and intact acrosome; II - sperm wi...

Eliška Špaleková; Alexander Makarevich; Elena Kubovi?ová; Juraj Pivko

2013-01-01

71

Total Antioxidant Capacity and Lipid Peroxidation in Semen of Patient with Hyperviscosity  

Directory of Open Access Journals (Sweden)

Full Text Available Semen hyperviscosity (SHV is one of the factors involved in deficiency in sperm function. This research aimed to evaluate seminal plasma total antioxidant capacity (TAC and malondialdehyde (MDA levels in infertile patients with hyperviscous and non-hyperviscous semen samples to understand whether hyperviscous semen is associated with oxidative damage in infertile subjects. In this cross sectional study, 59 semen samples were provided by fertile (n=12 individuals as control, infertile patients with normal viscosity (n=25 and infertile patients with hyperviscosity (n=22. After semen parameters examination, semen viscosity was studied by glass pipettes. Seminal plasma TAC and MDA levels were measured by ferric reducing of antioxidant power (FRAP and thiobarbituric acid reaction (TBAR methods, respectively. A probability less than 0.05 was considered statistically significant throughout the article. The mean of sperm parameters including: counts, motility and normal morphology in patients with hyperviscosity were significantly lower than those in non-hyperviscosity patients (p<0.05, p<0.01 and p<0.001, respectively. The mean of seminal plasma TAC value in seminal plasma of non-hyperviscosity patients (1710.31 ± 458.67 ?mol/l was significantly (p<0.01 higher than that of hyperviscosity group (1230.25 ± 352 ?mol/l. A trend toward a higher mean of seminal plasma MDA value was estimated for hyperviscous group compared with non-hyperviscous (1.01 ± 0.41 nmol/ml vs. 0.94 ± 0.28 nmol/l; however, it was nonsignificant. Hyperviscous semen impairs seminal plasma TAC which is eventually associated with sperm membrane lipid peroxidation.

Issa Layali

2015-01-01

72

Semen analysis and sperm function testing  

OpenAIRE

Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis of semen as described in the new World Health Organization (WHO) manual for semen analysis. The mos...

Franken, Daniel R.; Oehninger, Sergio

2011-01-01

73

Histochemical demonstration of zinc ions in ejaculated human semen  

DEFF Research Database (Denmark)

A revised in-vitro technique for autometallographic demonstration of chelatable zinc in the human ejaculate is presented, and the localization of the loosely bound pool of zinc ions is described in semen smears and at the ultrastructural level. In semen smears, black autometallographic (AMG) grains indicated the presence of zinc ions dispersed between the spermatozoa. These AMG grains have the same size as grains associated with the sperm tail and may have the same origin. EM analysis of AMG-developed smears fixed in osmium suggested that the detected zinc ions might be related to huge protein molecules present in semen and adhering to the surface of the spermatozoa. Spermatozoa in AMG-stained smears exhibited zinc ions in the midpiece and head, and also joined to the membrane of the tail. Washed spermatozoa exhibited zinc ions only within the midpiece. Ultrastructurally, they were found located in the helecine mitochondria. A few grains were found in the acrosome of the washed spermatozoa. Treatment with thechelating agent DEDTC resulted in complete bleaching of the zinc staining. These findings and the fact that calcium EDTA acid blocks the plasma and surface staining, but not the acrosomal and mitochondrial staining, suggest that chelatable zinc ions exist in two separate pools in human semen.

Stoltenberg, M; SØrensen, M B

1997-01-01

74

Fertility and uterine hemodynamic in cows after artificial insemination with semen assessed by fluorescent probes.  

Science.gov (United States)

Fluorescent probes (propidium iodide, Hoechst 33342, fluorescein isothiocyanate-conjugated Pisum sativum agglutinin, and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide) were used to simultaneously evaluate the integrity of plasma and acrosomal membranes as well as mitochondrial membrane function in cryopreserved bovine semen and to verify its influence on fertility and postinsemination uterine vascularization. One hundred eighty-two Nellore cows were distributed for artificial insemination (AI) using semen batches separated according to the cell percentage presenting intact plasma membrane, intact acrosome, and high mitochondrial function (IPIAH): group G (44.5% IPIAH, n = 68), group M (23.0% IPIAH, n = 56), and group R (8.5% IPIAH, n = 58). The uterine hemodynamic was evaluated by Doppler sonogram in three periods: 30 hours before AI, 4 and 24 hours after AI were considered the resistance index and the uterine vascularization score. The pregnancy rate of group G (64.7%) was greater (P > 0.05) compared with group R (36.2%), but both did not differ from group M (50.0%). There was no effect (P > 0.05) of semen quality on uterine vascularization. Greater vascularization was noticed 4 hours after AI than 30 hours before and 24 hours after AI. Semen evaluation using fluorescent probes contributes to predicting fertilizing potential of semen. The use of semen with less percentage of IPIAH sperm does not alter uterine hemodynamic in cows. PMID:25023296

Oliveira, Bruna Marcele; Arruda, Rubens Paes; Thomé, Helder Esteves; Maturana Filho, Milton; Oliveira, Guilherme; Guimarães, Carina; Nichi, Marcílio; Silva, Luciano Andrade; Celeghini, Eneiva Carla Carvalho

2014-09-15

75

Evaluation of CD52 positive sperms in subfertile human semen samples: Is there any relationship with main semen parameters?  

Science.gov (United States)

Background: Sperm maturation and sperm membrane integration are the most important elements in male fertility. CD52 is one of the antigens. CD52 is a GPI (glycosylphosphatidylinositol) anchored that express on lymphocytes and epididymal cells. This antigen bind to sperm membrane during transition sperm from epididymal duct as well as its relationship with semenogelins in human seminal plasma. The aim of this study was to obtain any association between the percentage of CD52 positive sperms with main semen parameters such as percentage of motile sperms, percentage of sperm with normal morphology, and the presence of normal viscosity. Materials and Methods: Semen samples from subfertile men were analyzed, the samples totally were 45 that divided according to their motility into three groups, first one, more than 40%, second one 10-40%, and the third one under 10% total motility. Fifteen samples in each group were evaluated by semen analysis according to WHO 2010 guidelines for infertility laboratory. Sperms were washed by Ham's F-10 and immunostaining with the monoclonal antibody CAMPATH-1G and then analyzed by flow cytometry. We compared each of the groups based on their motility and the data were analyzed by SPSS 20. Results: Correlation between CD52 labeling and sperm motility was negatively significant, in the second group (r = –0.592, P = 0.020) and in the third group (r = –0.805, P = 0.00). Conclusion: Our results showed that the correlation between CD52 labeling and sperm motility was negatively significant, but we did not observe any relation with other semen parameters, such as sperm normal morphology, sperm concentration, and semen viscosity. PMID:24949295

Aboutorabi, Roshanak; Mazani, Fatemeh; Rafiee, Laleh

2014-01-01

76

Fennel (Foeniculum vulgare) provides antioxidant protection for boar semen cryopreservation.  

Science.gov (United States)

Boar semen is extremely vulnerable to cold shock and it is also sensitive to peroxidation due to the high content of unsaturated fatty acids in the plasma membrane. Antioxidants exert a protective effect on the plasma membrane of frozen boar sperm. Fennel has been shown to contain antioxidant substances. Therefore, this study was performed to evaluate the effect of different concentrations of fennel added to the freezing extender on boar semen quality and lipid peroxidation after thawing. Semen collected from four boars was cryopreserved in lactose-egg-yolk extender or in the same extender with varying concentration of fennel essences: low (LF); medium (MF); high (HF). Analysis of data clearly indicated that higher concentrations of fennel produced significant improvement in total motility. Moreover, when fennel was included in the extender, a dose-dependent tendency to increase sperm viability was observed. In contrast, the addition of fennel had no effect on acrosome integrity or hypoosmotic swelling test (HOST) compared with the control. Malondialdehyde (MDA) formation decreased significantly in fennel groups, yielding similar results for MF and HF. Fennel seems a new antioxidant for use in sperm cryopreservation, but its particular effects on sperm physiology must be further studied, especially the causes of motility stimulation and its effect on lipoxidation. PMID:22106928

Malo, C; Gil, L; Cano, R; González, N; Luño, V

2012-05-01

77

Effects of seasons on some semen parameters and bacterial contamination of Awassi ram semen.  

Science.gov (United States)

The objectives of the present study were to determine the effects of season on some semen parameters and bacterial contamination of Awassi ram semen. Semen samples from six mature Awassi rams were used in this study. Semen collection was performed with artificial vagina every week, from September 2009 to October 2010. Volume, sperm concentration, mass motility, individual motility, percentage live sperm and sperm abnormalities were evaluated. Moreover, determination of viable bacterial count of the rams was also recorded weekly. Higher (p semen volume in the hot summer and spring months was observed of August (1.55 ± 0.08 ml) and March (1.27 ± 0.15 ml). Sperm concentration was highest (p ram semen quality, and summer high temperature in northern Iraq has no effect on Awassi ram semen. There is a significant effect of season on bacterial count on Awassi ram semen. PMID:21883516

Azawi, O I; Ismaeel, M A

2012-06-01

78

Effect of centrifugation and sugar supplementation on the semen cryopreservation of captive collared peccaries (Tayassu tajacu).  

Science.gov (United States)

The present study is aimed at evaluating the effect of centrifugation for seminal plasma removal and the supplementation of fructose or glucose to the Tris-based extender on the kinematic patterns of the motility parameters of frozen-thawed semen obtained from captive collared peccaries (Tayassu tajacu). Semen samples (n = 14) were collected from 10 sexually mature male collared peccaries by electroejaculation. These samples were further evaluated for parameters such as motility, vigor, sperm viability, membrane integrity, and sperm morphology. The samples were divided into four aliquots, and only two of these aliquots were centrifuged. The semen aliquots (centrifuged and raw semen samples) were diluted in Tris-based extenders supplemented with fructose or glucose. Egg yolk (20%) and glycerol (3%) were added to all the samples which were cryopreserved in liquid nitrogen and thawed at 37 °C/1 min. The frozen-thawed semen was evaluated for the same parameters described for the fresh semen. On the other hand, the kinematic motility patterns were evaluated by a computer-aided system. After thawing, it was observed that the values for the total sperm motility were around 30% for all the samples. A negative effect of centrifugation was verified for parameters such as sperm morphology, linearity, straightness, and beat cross frequency (P 0.05). In conclusion, it is not recommended to centrifuge the ejaculates from collared peccaries prior to conducting the cryopreservative procedures using a Tris-based extender supplemented with fructose or glucose. PMID:20858475

Castelo, T S; Bezerra, F S B; Lima, G L; Alves, H M; Oliveira, I R S; Santos, E A A; Peixoto, G C X; Silva, A R

2010-12-01

79

The effect of dietary n-3 polyunsaturated fatty acids supplementation of rams on semen quality and subsequent quality of liquid stored semen.  

Science.gov (United States)

The objective of this study was to examine the effect of dietary n-3 polyunsaturated fatty acid (PUFA) supplementation of rams on semen quality and subsequent sperm function of liquid stored semen. Mature rams of proven fertility were individually housed and were blocked according to breed, body weight, and body condition score and randomly allocated within block to one of two dietary treatments (N = 7 per treatment). Rams were offered a base diet of hay and concentrate, with the concentrate enriched with either: (1) saturated palmitic acid (CON) or (2) high n-3 PUFA fish oil (FO) supplements. Both lipid supplements were added at 2% (wt/wt) of the total diet as fed and both were partially rumen-protected. The animals were fed their respective diets for a total of 9 weeks and blood samples were collected on weeks 0 (pre-experimental), 4, and 9, relative to initial allocation of diet (week 0), for measurement of plasma concentration of fatty acids, metabolites, insulin like growth factor 1 (IGF-1) and insulin. Semen was collected from each ram (on 1 day in each week) in weeks 4, 5, 7, 8, and 9, and each ejaculate was assessed for volume, wave motion, and concentration of sperm, after which it was diluted in a skim milk-based extender and stored at 4 °C. A second ejaculate was collected on weeks 4, 7, and 9, centrifuged, and the sperm frozen for subsequent lipid analysis. A sample of semen from each ram was assessed at 24, 48, and 72 hours after collection for sperm progressive linear motion, ability to penetrate artificial mucus, and the ability to resist lipid peroxidation (at 24 and 48 hours only) using the thiobarbituric acid reactive substances assay. There was no effect of diet on plasma insulin concentrations or on any of the metabolites measured, however, there was a diet by week interaction for plasma IGF-1 concentration (P rams having higher IGF-1 concentrations on week 9 compared with the control treatment (P rams (P rams supplemented with FO yielded a higher semen concentration (P semen quality parameters including semen volume, wave motion, progressive linear motion, ability to penetrate artificial mucus, or ability to resist lipid peroxidation. In conclusion, dietary supplementation of rams with n-3 PUFA successfully increased the n-3 PUFA content of plasma and sperm but has limited effects on the quality of liquid stored semen. PMID:24100164

Fair, S; Doyle, D N; Diskin, M G; Hennessy, A A; Kenny, D A

2014-01-15

80

Semen Allergy Manifesting As Chronic Pruritus Vulva  

Directory of Open Access Journals (Sweden)

Full Text Available A young woman of 24 with personal and family history of atopy development pruritus vulva each time after sexual intercourse with her husband. History of urticaria of sites of contact with semen on her thighs gave suspicion of contact urticaria. Positive wheal and flare response to pin prick test with semen, excellent therapeutic response to topical steroid and oral Cetirizine and non- recurrence of the problem after using condom by her husband confirmed the diagnosis of semen allergy.

Pavithran K

1997-01-01

81

Semen Allergy Manifesting As Chronic Pruritus Vulva  

OpenAIRE

A young woman of 24 with personal and family history of atopy development pruritus vulva each time after sexual intercourse with her husband. History of urticaria of sites of contact with semen on her thighs gave suspicion of contact urticaria. Positive wheal and flare response to pin prick test with semen, excellent therapeutic response to topical steroid and oral Cetirizine and non- recurrence of the problem after using condom by her husband confirmed the diagnosis of semen allergy.

Pavithran K

1997-01-01

82

Caracterização de proteínas do plasma seminal e sua relação com parâmetros de qualidade do sêmen criopreservado em ovinos Characterization of seminal plasma proteins and its relationship with quality parameters of frozen semen in ram  

OpenAIRE

Os objetivos deste trabalho foram analisar o perfil proteico do plasma seminal ovino e identificar proteínas relacionadas com a congelabilidade do sêmen que possam ser utilizadas como marcadores para essa característica. Foram utilizados os ejaculados de cinco reprodutores, nos quais foram realizadas avaliações espermáticas e dos quais os plasmas seminais obtidos por centrifugação foram submetidos à eletroforese bidimensional em gel de poliacrilamida. Foram identificados 92 spots, co...

Priscilla Pereira Moura; Maurício Machaim Franco; Thiago Antônio de Souza Nascimento Silva; Thales Lima Rocha; Diogo Ramos Leal; Pedro Ivo Braga Passos; Jairo Pereira Neves

2010-01-01

83

Rapid detection of semenogelin by one-step immunochromatographic assay for semen identification.  

Science.gov (United States)

To identify semen in forensic samples, we developed an analytical system for one-step immunoassay that has been constructed using the concept of immunochromatography and can identify semenogelin (Sg), which originates in the seminal vesicles. The system employed monoclonal antibody (mAb) and polyclonal antibody (pAb) against recombinant Sg-II (63 kDa), which has been synthesized in insect cells using baculovirus. The two antibodies bound with the seminal plasma motility inhibitor (SPMI; 14 kDa) as a final fragment peptide of Sg. The test stick is based on the sandwich technique using the above antibodies. When serial dilutions of seminal plasma were analyzed using this test stick, the intensity of a clear immunoreactive signal peaked at 2000-fold dilution. Thereafter, the signals decreased slowly but still persisted up to 400,000-fold dilution. The Sg antigen was undetectable in saliva, urine, breast milk, serum or vaginal secretions. Also, the test stick shown did not react with animal semen samples, such as those from horses, dogs, swine and bulls. When semen samples, diluted 100,000-fold from 100 men were tested, the Sg antigenic activity was detectable in all samples. In addition, the specificity and sensitivity of the test stick for identification of semen were demonstrated by comparative forensic studies. We conclude that this immunoassay method is a useful confirmatory test for the identification of semen. The immunochromatographic system for forensic testing or research use will become available commercially soon. PMID:15099762

Sato, Itaru; Kojima, Koichiro; Yamasaki, Tadashi; Yoshida, Kaoru; Yoshiike, Miki; Takano, Shoichi; Mukai, Toshiji; Iwamoto, Teruaki

2004-04-01

84

The Viability of Local Ram Semen in Tris Buffer With Three Different Egg Yolks  

Directory of Open Access Journals (Sweden)

Full Text Available Egg yolk consisted of lecithin and phospholipids are one of the most commonly used components that will protect spermatozoa against cold shock during cooling and freezing. The aim of this study was to evaluate the effect of different hen egg yolk on Tris extender on the freezability of local ram semen. Semen from six sexually matured local rams was collected weekly using artificial vagina. Collected semen was evaluated macroscopically and microscopically and extended using tris extender consisted of 20% (v/v regular egg yolk (TRCEY, native egg yolk (TNCEY, omega-3 hen egg yolk (TOEY and 6% (v/v glycerol. Those were packed in 0.25 ml straws, equilibrated at 5oC for 3 hours, frozen and stored in nitrogen tank for 24 hours, and thawed at 37oC for 30 second. The result of the experiment showed that there were no significant differences on the sperm motility and the number of living sperm. Percentage of plasma membrane intact in TOEY (60.3% was significantly higher compared to TREY (56.9% and TNEY (55.6%. In conclusion, the addition of omega 3 egg yolk in Tris extender protects plasma membrane better than the regular or native hen egg yolk. (Animal Production 13 (1:39-44 (2011Key Words: ram semen, egg yolk, frozen semen

WMM Nalley

2011-01-01

85

Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo Addition of seminal plasma to frozen-thawed semen and pregnancy rate of fixed time inseminated ewes  

OpenAIRE

Avaliou-se o efeito da adição de plasma seminal ovino ao sêmen descongelado sobre a taxa de prenhez de ovelhas em rebanho comercial. Cento e setenta e quatro ovelhas cruza Texel foram distribuídas em quatro tratamentos: T1) inseminação artificial cervical (IAC) com sêmen descongelado (SD) diluído em solução tampão fosfato salino (PBS); T2) IAC com SD e adição de plasma seminal ovino; T3) grupo-controle I: IAC com sêmen fresco diluído em PBS; T4) grupo-controle II: inseminação...

Prado, O. R.; Bastos, G. M.; Monteiro, A. L. G.; Saab, B. B.; Gilaverte, S.; Pierobom, C. C.; Hentz, F.; Martins, L. H. S.; Silva, C. J. A.; Dranca, G. S.; Stivari, T. S. S.; Cerqueira, G.

2013-01-01

86

Naturally and stimulated levels of reactive oxygen species in cooled stallion semen destined for artificial insemination.  

Science.gov (United States)

The decrease in foaling rates after artificial insemination with cooled semen warrants the search for new predictors of fertility. The objectives were to investigate levels of naturally occurring reactive oxygen species (ROS) in cooled, stored stallion semen doses for artificial insemination (AI), and their relationship with parameters of semen quality and with pregnancy rate. Semen was collected from warmblood stallions (n=15) and used to prepare commercial semen doses for AI. Sperm quality was evaluated after cooled transport to the laboratory overnight. The results were correlated with observed foaling and pregnancy rates. Hydroethidine and dichlorodihydrofluorescein diacetate were used as indicators for the ROS superoxide and hydrogen peroxide, respectively. Sperm morphology, motility, plasma membrane integrity and chromatin integrity were also evaluated. These variables were correlated with each other and with pregnancy rates. We found a high inter-individual variation in the ROS levels between stallions. The proportion of live, hydrogen peroxide-negative spermatozoa was correlated with progressive motility, whereas live hydrogen peroxide-negative spermatozoa and chromatin damage were negatively correlated, indicating that low levels of hydrogen peroxide were correlated with good chromatin integrity. The percentage of dead hydrogen peroxide-positive sperm was negatively related to the foaling rate. The negative relationships were stronger when combining results from both assays for ROS. These results for stored semen samples indicate that high individual variation exists for superoxide and hydrogen peroxide measurements, and that ROS status can influence sperm quality. Thus, ROS may be some of the factors influencing fertility. Moreover, combinations of ROS variables improved the correlation with fertility, indicating the usefulness of including these variables in a future model for prediction of the fertility of a semen sample. PMID:24916995

Johannisson, A; Lundgren, A; Humblot, P; Morrell, J M

2014-10-01

87

Effects of incubation temperature and semen pooling on the viability of fresh, chilled and freeze-thawed canine semen samples.  

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This study assessed the effects of different incubation temperatures on semen viability and the influence of pooling on semen longevity. In experiment 1, semen samples were collected from five dogs, individually processed (individual semen: IS) and then aliquots from each male were pooled (pooled semen: PS). Semen samples (IS and PS) were diluted in a Tris-glucose-yolk extender and preserved as fresh (37 and 25°C) and chilled semen (4°C). Sperm motility and the percentages of sperm abnormalities and acrosome membrane integrity were assessed for 24 h. Storage at 25 or 4°C for the first 24 h yielded similar semen quality, but incubation at 37°C caused drastic reduction in sperm motility from 8 h of incubation onwards. In experiment 2, the semen was processed in the same way to that of experiment 1 and then preserved at 25 or 4°C until semen inactivation. Semen that was incubated at 25°C became completely inactive after 3-4 days of storage, while semen that was preserved at 4°C presented with more gradually decreased sperm motility (mean values of 40-60% for the first 8 days). In addition, the mixing of semen was only observed to influence the sperm quality of the samples stored at 4°C. In experiment 3, semen was collected from five dogs, pooled and frozen in liquid nitrogen; after thawing, it was preserved at 37, 25, 15 and 4°C, and the sperm quality was defined. The motility of the freeze-thawed semen samples decreased quickly in the first 4 h after thawing, regardless of the preservation temperature of the thawed semen. This study confirmed that semen preserved at 37°C should be used within a maximum of 12 h, while the semen stored at 25°C shows acceptable quality for 24 h. Chilled semen presented highest most sustainable quality, especially when semen is processed as pooled semen. PMID:22458911

Batista, M; Santana, M; Alamo, D; González, F; Niño, T; Cabrera, F; Gracia, A

2012-12-01

88

Organic selenium supplementation increases PHGPx but does not improve viability in chilled boar semen.  

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This study evaluated the effects of dietary organic selenium (Se) on viability of chilled boar semen. Twelve boars were divided into three groups: control (CON), 0.3 mg kg(-1) sodium selenite; inorganic (INO), 0.5 mg kg(-1) sodium selenite and organic (ORG), 0.5 mg kg(-1) Se yeast. The experiment was conducted within 10 weeks, and analysis was performed fortnightly, in storage semen by 72 h. No effect was observed on motility; however, straightness and linearity percentages were higher (P Percentages of cells with both plasma and acrosomal intact membranes, lipidic membrane peroxidation and mitochondrial membrane potential were similar on all treatments. Animals receiving CON diet presented higher (P higher (P < 0.05) in animals that received ORG in comparison with INO group. In conclusion, organic selenium supplementation increases PHGPx but does not improve chilled semen viability in 72 h. PMID:24428535

Martins, S M M K; De Andrade, A F C; Zaffalon, F G; Bressan, F F; Pugine, S M P; Melo, M P; Chiaratti, M R; Marino, C T; Moretti, A S; Arruda, R P

2015-02-01

89

Potential factors affecting semen quality in the Asian elephant (Elephas maximus  

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Full Text Available Abstract Background One of the major obstacles in using artificial insemination to manage genetics of elephant population in captivity is the large variations in semen quality among ejaculates within the same and among individuals. The objectives of this study were to determine the influences of (1 age (2 seasonality (3 and circulating testosterone (SrTest, triiodothyronine (SrT3 and tetraiodothyronine (SrT4, as well as seminal (4 testosterone (SpTest, zinc (SpZn and protein (SpTP on semen quality in the Asian elephant Methods Analyses, including motility, viability and morphology were performed in semen samples collected twice monthly from 13 elephant bulls (age range, 10-to 72-years by manual stimulation between July 2004 and June 2005. Serum samples obtained monthly were assessed for SrTest, SrT3, SrT4, and seminal plasma samples were evaluated for, SpTest, SpZn and SpTP. Results The highest semen quality was observed at age 23 to 43 years. Percentages of progressive motility and viable sperm were lowest at age 51 to 70 years (P Conclusion This study indicates that age and seasonality had influence on semen characteristics in the Asian elephant. The knowledge obtained in this study will improve our understanding of the reproductive biology of this species.

Pongsopavijitr Pornsawan

2008-03-01

90

Semen analysis under photochemotherapy (PUVA-therapy)  

International Nuclear Information System (INIS)

In 9 male patients with psoriasis vulgaris a semen analysis before and during photochemotherapy with 8-methoxypsoralen and UVA (PUVA) was performed to rule out drug-induced toxic damage of spermatogenesis or impairment of fertility due to scrotal hyperthermia. Two hours after oral application of 40-60 mg 8-methoxypsoralen the patients had been irradiated in UVA high intensity treatment units. PUVA-treatments were performed four times weekly until total body clearing was achieved. For complete remission 13-26 (mean 20.5) PUVA-treatments were necessary. Corresponding total UVA-doses were 35.3 - 191.0 (mean 83.2) Joule/cm2. The investigated parameters total motility, progressive motility, spermatozoa density, total spermatozoa count, spermatozoa morphology, and seminal plasma fructose remained unchanged. Only the volume of the ejaculate showed a small decrease during 3 months of therapy. From this pilot study there is no evidence, that PUVA-therapy leads to an impairment of fertility in male patients within their reproductive age. (orig.)

91

Reduction of concentrate for bovine sires: Influence on metabolic status and semen quality under production conditions  

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The effects of reduced concentrate fed in rations of Holstein Friesian bulls for artificial insemination was evaluated with respect to metabolic status, sexual behaviour, semen production and semen quality during one year. In the first of two studies, twenty bulls were fed diets based on hay, green forage and concentrate according to the standard nutrient requirements for dairy cattle in artificial insemination centres. Bulls were divided into two groups: Group 1 (n = 10, control, 5 kg concentrate) and Group 2 (n = 10, experimental, 1 kg concentrate). Feed, blood semen samples were taken for bromatological analysis, metabolic profile and semen evaluation, respectively. Group 2 had lower plasma concentrations of urea (P<0.001), calcium (P<0.05) and phosphorous (P<0.01). Urea were below the reference range. Season of the year affected lipid metabolite concentrations (P<0.001) and osteotrophic minerals (P<0.05 to P<0.001). Group 2 had better production and quality of semen than did Group 1. In the second study, five bulls were fed as the experimental group in the first study. Time of sampling, season of the year and sire affected the hormonal secretion pattern (P<0.001). There were no differences in testoterone and LH plasma concentrations before and after mounting; however, cortisol concentrations showed a significant raise during the period of maximum excitation. Individual secretion patterns varied between bulls and were related to pathological morphology of reproducted to pathological morphology of reproductive and endocrine organs. The effect of sire was significant on all the indicators of the sperm production, except to percentage of live sperm. Season of the year significantly affected sperm concentration and number of doses of extended sperm produced. It is concluded that a reduction of concentrate in the diet did not affect the metabolic status, sexual behaviour, semen production or sperm quality of sires. 29 refs, 2 figs, 4 tabs

92

Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality  

OpenAIRE

In captivity, male Asian elephants often yield poor quality semen after transrectal manually assisted semen collection; however, the reasons for the disappointing semen quality are not clear. Here we test the hypothesis that accumulation of senescent spermatozoa is a contributory factor, and that semen quality can therefore be improved by more frequent ejaculation. To this end we investigated the effect of collecting semen five times on alternate days, after a long period of sexual rest, on s...

Imrat, Podjana; Mahasawangkul, Sittidet; Thitaram, C.; Suthanmapinanth, P.; Kornkaewrat, K.; Sombutputorn, P.; Jansittiwate, S.; Thongtip, N.; Pinyopummin, A.; Colenbrander, B.; Holt, W. V.; Stout, Tom A. E.

2014-01-01

93

Alternatives to Antibiotics in Semen Extenders: A Review  

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Full Text Available Antibiotics are added to semen extenders to be used for artificial insemination (AI in livestock breeding to control bacterial contamination in semen arising during collection and processing. The antibiotics to be added and their concentrations for semen for international trade are specified by government directives. Since the animal production industry uses large quantities of semen for artificial insemination, large amounts of antibiotics are currently used in semen extenders. Possible alternatives to antibiotics are discussed, including physical removal of the bacteria during semen processing, as well as the development of novel antimicrobials. Colloid centrifugation, particularly Single Layer Centrifugation, when carried out with a strict aseptic technique, offers a feasible method for reducing bacterial contamination in semen and is a practical method for semen processing laboratories to adopt. However, none of these alternatives to antibiotics should replace strict attention to hygiene during semen collection and handling.

Jane M. Morrell

2014-12-01

94

DISINFECTION BYPRODUCTS AND HUMAN SEMEN QUALITY  

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This project, also called the Healthy Men Study will examine potential associations between human exposure to drinking water disinfection byproducts, particularly haloacetic acids (HAAs) and trihalomethanes (THMs), and male reproductive health as indicated by semen quality. Sinc...

95

Artificial insemination of cranes with frozen semen  

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For the first time (1978) artificial insemination (AI) with frozen greater sandhill crane (Grus canadensis tabida) semen resulted in fertile eggs and chicks. During the 2 year (1977-78) study, 6 of 27 eggs produced were fertile. Three chicks hatched. Semen samples used for insemination were frozen and stored in liquid nitrogen for two months or less. Recent improvements in the laboratory indicated that a more effective sample can be prepared and greater fertility rates should be expected.

Gee, G.F.; Sexton, T.J.

1979-01-01

96

Study of enzyme activities and protein content of beluga (Huso huso) semen before and after cryopreservation.  

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Knowledge gained regarding the biochemical processes that occur during sperm collection, processing and freezing-thawing might improve current sperm cryopreservation techniques. In our present study, we determined the effect of cryopreservation on the total protein concentration (TP) and the activities of certain enzymes in semen samples from the beluga (Huso huso). The TP content of the seminal plasma of fresh semen was 0.47 ± 0.026 g/l, and the TP after cryopreservation was 1.86 ± 0.6 g/l. The activities of acid phosphatase (0.82 ± 0.042 U/l), lactate dehydrogenase (234.4 ± 19.4 U/l), arylsulfatase (143.1 ± 32.5 U/l) and ?-N-acetylglucosaminidase (58.39 ± 4.14 U/l) in the seminal plasma of fresh semen were significantly lower than those in the supernatant of frozen-thawed semen samples (7.43 ± 0.64, 3224.6 ± 167.2, 422.6 ± 21.3 and 90.2 ± 5.37 U/l respectively). These parameters may be useful as biomarkers for estimating damage to the cell membrane of spermatozoa caused by freezing-thawing. PMID:24780122

Aramli, M S

2015-02-01

97

Effects of dietary vitamin supplementation and semen collection frequency on reproductive performance and semen quality in boars.  

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The present study was undertaken to assess the relevance of increasing the daily provision of dietary vitamins on vitamin metabolic status and semen characteristics of boars under controlled and commercial conditions as well as to evaluate the efficiency of this vitamin supplement to allow boars to cope with intensive semen collection frequency. In the first experiment, 39 boars were allocated to 2 dietary treatments, a basal diet (control) and the basal diet supplemented with extra fat- and water-soluble vitamins (Vit). Within each treatment, boars were submitted to 2 regimens of semen collection frequency: 3 times per 2 wk (3/2) and 3 times per week (3/1) over a 12-wk period. Afterwards, all boars were intensively collected (daily) for 2 wk. A resting period of 4 wk followed, and all boars were collected 2 times per week. Thereafter, collection frequencies were reversed, and the same procedure was followed until the end of the intensive collection period. A second experiment was conducted in commercial conditions at a commercial stud, and 252 boars were randomly allocated to the control and Vit dietary treatments. All boars were collected 2 times per week over a 6-mo period. Classical measurements of ejaculate and sperm quality were assessed, and blood samples were collected throughout both experiments to quantify vitamin concentrations. In the first experiment, vitamin concentrations in blood and seminal plasma increased in Vit boars (P 0.14). The Vit supplement did not affect sperm production or sperm quality (P > 0.28), although semen volume increased during the 12-wk periods for Vit boars (P boars produced fewer doses per ejaculate than 3/2 boars (P boars compared with 3/2 boars. In the second experiment, blood plasma concentrations of vitamin B(9) were greater (P boars. The vitamin supplement did not increase sperm production of boars (P > 0.61). In conclusion, dietary supplements of fat- and water-soluble vitamins increase the amount of vitamins available for the animal, and the collection frequencies had no effect on vitamin status. Moreover, in spite of an effect on the ejaculate volume, the dietary supplement of extra vitamins had no effect on sperm production or quality. PMID:19286814

Audet, I; Bérubé, N; Bailey, J L; Laforest, J-P; Matte, J J

2009-06-01

98

Rooster semen cryopreservation: effect of pedigree line and male age on postthaw sperm function.  

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The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in preservation of commercial genetic stocks. Our objective was to evaluate the cryosurvival of semen from 8 pedigreed layer lines at 2 different ages: the onset and end of commercial production. Semen from 160 roosters (20/line) was frozen individually with 11% glycerol at 6 and 12 mo of age. Glycerol was removed from thawed semen by Accudenz gradient centrifugation. The viability of thawed sperm from each male was determined using fluorescent live-dead staining and flow cytometry; sperm velocity parameters were measured using computerized motion analysis. The fertilizing ability of thawed sperm was evaluated in vitro by assessing hydrolysis of the inner perivitelline membrane. The postthaw function of sperm from the elite lines varied widely, despite the fact that fresh semen from all of these lines typically yielded high fertility rates. The percentage of thawed sperm with intact plasma membranes ranged from 27.8 + or - 2.1 to 49.6 + or - 1.9 and varied among lines and between age groups. Thawed sperm from 2 lines consistently demonstrated the highest and lowest motility parameters, whereas the velocity parameters of the remaining 6 lines varied widely. The mean number of hydrolysis points per square millimeter of inner perivitelline membrane ranged from 12.5 + or - 4.1 (line 2) to 103.3 + or - 30.2 (line 6). Age effects were observed for 4 out of 8 lines; however, improved postthaw sperm function at 12 mo of age was not consistent for all 3 assays. These results demonstrate variability among pedigreed lines in withstanding glycerol-based semen cryopreservation and provide a model for delineating genotypic and phenotypic factors affecting sperm cryosurvival. PMID:20371849

Long, J A; Bongalhardo, D C; Pelaéz, J; Saxena, S; Settar, P; O'Sullivan, N P; Fulton, J E

2010-05-01

99

Comparison of three diluents for the storage of fresh bovine semen.  

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In New Zealand, 95% of the semen used for artificial insemination in cattle is processed as liquid semen. Storage of liquid semen for up to 3 days in Caprogen) diluent enables a 10-fold reduction of the insemination dose, compared to frozen-thawed semen, without a reduction in fertility. In this Caprogen) diluent spermatozoa are stored under N2 gas in the presence of catalase. However, a new diluent (CEP-2), which was originally based on the biochemical composition of bovine cauda epididymal plasma, could become an appropriate alternative to Caprogen. In this study, the effect of addition of catalase to bovine spermatozoa stored for 6 days in CEP-2 diluent under aerobic and anaerobic conditions was evaluated and compared with a Tris diluent. Additionally, the quality and in vitro fertilizing capacity of fresh bovine semen stored for 6 days at 5 degrees C in the Triladyl, CEP-2 (without catalase and N2 gas) and Caprogen diluent were compared. Addition of 4.5 mg/mL catalase to CEP-2 diluent under aerobic and anaerobic conditions had no effect on sperm quality. Spermatozoa stored in CEP-2 diluent moved faster and straighter than spermatozoa stored in Triladyl or Caprogen diluent. The in vitro fertilization and polyspermy rates did not differ significantly between spermatozoa stored for 6 days at 5 degrees C in CEP-2 and Caprogen diluent, but were significantly lower for spermatozoa stored in Triladyl diluent. We can conclude that based on the in vitro results, the CEP-2 diluent is a better diluent than Triladyl and a good alternative to the Caprogen diluent for long term storage of fresh bovine semen at 5 degrees C. To confirm these promising in vitro results further in vivo experiments are required. PMID:15629807

Verberckmoes, Steven; Van Soom, Ann; Dewulf, Jeroen; de Kruif, Aart

2005-02-01

100

Semen searching when sperm is absent.  

Science.gov (United States)

Sexual assault cases have varying factors that may mask semen findings when analysing evidence at the forensic laboratory. Semenogelin (Sg) is a potential marker for the identification of semen even at azoospermy or when few sperm cells are found. The current study examined Sg in normospermic and azoospermic donors as an internal evaluation of sensitivity, specificity and interference. The impact of a historical review of 53 judicial sexual assault cases over a five-year period was also analysed. The use of varying tests was of importance to prioritize certain samples within cases. Semen findings by Sg were then compared to prostate-specific antigen (PSA), phosphatase enzyme (AP) and Y-chromosome presence, the latter being used in an attempt to link semen fluid identification with obtaining a male DNA profile. Test findings were the highest ever registered for Sg (1:400,000), PSA (1:800,000), AP (1:25,000) and sperm cytology (SC) (1:50,000). Our results demonstrated the usefulness of using the Sg marker to avoid a false semen-negative result (6% cases), particularly in cases where sperm was absent or scarce (11% spermatozoa positive cases). Results were expressed in categories according to the set: Sg-PSA-AP. Thus, categories I (full positive, 46%), VI (full negative, 27%) and III (Sg/PSA positive; 11%) were the most frequent and Y-chromosome was obtained in 59%, 12% and 12% ratios, respectively. In conclusion, Sg was recommended for the workflow procedure of semen investigation when sperm absence is expected either from azoospermic/oligospermic or normospermic semen, especially before/after ejaculation. PMID:25753997

Martínez, Pilar; Santiago, Begoña; Alcalá, Belén; Atienza, Inmaculada

2015-03-01

101

19 CFR 12.32 - Honeybees and honeybee semen.  

Science.gov (United States)

...Duties 1 2010-04-01 2010-04-01 false Honeybees and honeybee semen. 12.32 Section 12.32 Customs Duties... Wild Animals, Birds, and Insects § 12.32 Honeybees and honeybee semen. (a) Honeybees from...

2010-04-01

102

Effects of Exogenous Glutathione Supplementation in Biocell® Extender on Quality of Cryopreserved Buffalo (Bubalus bubalis Semen  

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Full Text Available The study was designed to investigate the effect of exogenous glutathione supplementation in soybean based extender Bioxcell® extender on post thaw semen quality of buffalo (Bubalus bubalis. Split pooled ejaculates (n = 6, possessing >70% visual sperm motility were extended at 37°C with different levels of glutathione (0.0, 0.5, 1 and 2 mM in Bioxcell® extender. Semen was cooled to 4°C in 2 h, equilibrated at 4°C for 4 h, filled in 0.5 mL straws and frozen in a programmable cell freezer before plunging into liquid nitrogen. Thawing of frozen semen was performed after 72 h at 37°C for 30 sec. Sperm motion characteristics, viability, plasma membrane integrity, acrosome morphology of each semen sample immediately after thawing and incubation for 2 h were assessed by using Computer assisted semen analyzer (SCA, eosin-nigrosin staining, Hypo Osmotic Swelling (HOS assay and phase contrast microscope, respectively. Results revealed that the addition 0.5 and 1.0 Mm of glutathione in Bioxcell® extender did not present any significant effect on overall and progressive motility as well as sperm motility characteristics (VAP, VSL, VCL, LIN and ALH, compared to the control groups (p>0.05. Immediately after thawing the proportion of post thaw sperm viability, plasma membrane integrity and normal apical ridge remained similar in all groups. However, glutathione supplementation of the extender with 2.0 mM concentration decreased sperm motility, viability at 0 and 2 h after thawing in a dose dependent manner compared to the control (p0.05. These results revealed that supplementation of the new commercial in soybean based extender Bioxcell® with glutathione did not improve sperm post thaw motility or acrosomal integrity.

Tohid Rezaei Topraggaleah

2012-01-01

103

Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen.  

Science.gov (United States)

The objective was to evaluate the effects of various antioxidants and duration of pre-freezing equilibration on cryopreservation of ram semen. Semen samples from four rams were pooled, diluted with Tris-egg yolk extender without antioxidants (control), or supplemented with reduced glutathione (GSH: 0.5, 1.0, and 2.0 mM), superoxide dismutase (SOD: 5, 10, and 20 U/mL), or catalase (CAT: 5, 10, and 20 U/mL), and cryopreserved, immediately after thermal equilibrium was reached at 5 °C (0 h), or 12 or 24 h after equilibration. Total antioxidant capacity was determined in the in natura extenders and after addition of semen samples for various durations of processing (fresh/dilute, throughout refrigeration, and post-thaw). Plasma membrane (PI-CFDA), acrosome integrity (FITC-PNA), and mitochondrial membrane potential (JC-1) were determined in fresh/diluted, refrigerated, and post-thaw samples. Post-thaw sperm motility was assessed with a computerized analysis system (CASA). There were no significant differences in acrosome damage or mitochondrial membrane potential after refrigeration and freeze-thaw, regardless of antioxidant addition. Sperm plasma membrane integrity was worse (P 0.05) among treatments after sperm addition or throughout the refrigeration and post-thaw. In conclusion, adding GSH, SOD or CAT did not increase the total antioxidant capacity of semen, nor did it enhance the quality of the post-thaw sperm. However, maintenance of ram semen at 5 °C for 12 h prior to cryopreservation reduced membrane damage of frozen-thawed sperm. PMID:21529917

Câmara, D R; Silva, S V; Almeida, F C; Nunes, J F; Guerra, M M P

2011-07-15

104

Semen quality and sedentary work position.  

Science.gov (United States)

Increased scrotal temperature can, in experimental settings, markedly disturb the production of semen. Sedentary work position may increase the temperature of the scrotum, but previous studies have failed to determine whether changes in scrotal temperature caused by sedentary work actually do affect semen quality. This study was carried out to elucidate the possible harmful effects of sedentary work on sperm count and other semen characteristics. In 1981-1983 a semen sample was obtained from 3119 men who attended an infertility workup in one of four Danish fertility centres. A total of 2517 men returned a postal questionnaire with information on life style, leisure time activities, occupational history and job duties. Information on job specific work position was obtained from The Danish Work Environment Cohort study 1990 (DWECS). In this analysis DWECS data for a total of 1747 men was included from men aged 18-39 years with >30 h of work per week. For all job titles represented in the DWECS, the mean proportion of sedentary work was estimated. The sperm cell concentration was 30.6 million/mL among men in the quintile with lowest job specific sedentary work compared with 40.5 million/mL in the highest quintile. The difference was, however, not statistically significant. Stratification on infertility period, educational level of the man, fertility centre, and fertility-related disease of the spouse did not influence the results. The analyses do not suggest that sedentary work is a risk factor for abnormal semen characteristics. PMID:14718040

Støy, Julie; Hjøllund, Niels Henrik I; Mortensen, Jens Tølbøll; Burr, Hermann; Bonde, Jens Peter

2004-02-01

105

Correlation of phthalate exposures with semen quality  

International Nuclear Information System (INIS)

Phthalates are widely used man-made chemical released in the environment and human exposure is mainly through diet. As the phthalate plasticizers are not covalently bound to PVC, they can leach, migrate or evaporate into the environment and as a result have become ubiquitously contaminants. The present study investigates the correlation, if any, between the phthalate esters (DEP, DEHP, DBP, DMP, DOP) and sperm mitochondrial status, ROS, LPO, SCSA, and sperm quality. The study was conducted in the urban/rural population of Lucknow visiting Obstetrics and Gynecology Department, CSMMU, Lucknow. Semen analysis was performed according to the WHO guidelines while phthalate analysis by HPLC and LPO by spectrophotometer and the sperm mitochondrial status, ROS, SCSA using flow cytometry. The questionnaire data showed no significant difference in the demographic characteristics among the groups. In general, urban population was found to have statistically significant higher levels of phthalate esters than the rural. Further, infertile men showed statistically significant (p < 0.05) higher levels of pollutants in the semen than fertile men. A negative correlation between semen phthalate level viz DEHP and sperm quality and positive association with depolarized mitochondria, elevation in ROS production and LPO, DNA fragmentation was established. The findings are suggestive that phthalates might be one among the contributing factors associated with the deterioration in semen qualiated with the deterioration in semen quality and these adverse effects might be ROS, LPO and mitochondrial dysfunction mediated

106

Relationship between Thyroid Profile and Semen Quality  

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Full Text Available Background: Endogenous hormones are critical to spermatogenesis and maintenance of male reproductive function. Follicle stimulating hormone (FSH, luteinizing hormone (LH, inhibin B and testosterone all serve important and well-known functions in the male hypothalamopituitary-gonadal axis and male reproduction. However, the potential relationship between other hormones, including thyroid hormones, and semen quality are still not completely understood. Thus in the present study an attempt has been made to report the degree of associations between thyroid hormones and semen quality. Methods: Fifty-five men were recruited from an infertility clinic between August 2010 to May 2011. Fresh semen samples were assessed for quality (concentration, motility and morphology and the serum levels of Tetraiodothyronine (T4, Triiodothyronine (T3, and Thyroid stimulation hormone (TSH were measured. Result & Conclusion: We have found that though men with abnormal semen profile had higher total T3, T4 concentrations and lower TSH concentrations compared to those with normal semen profile, only T4 showed significant increase and further it was found that only total T4 was significantly associated with asthenozoospermia. Further studies and observation are needed on a larger number of patients, to validate the correlation with Thyroid status and to justify the trial of a small dose of anti-thyroid drug in asthenozoospermic patients.

Manoj kumar Sharma, Deepak Parchwani, Pankaj Maheria, Amit Upadhyah

2012-01-01

107

Semen abnormalities with SSRI antidepressants.  

Science.gov (United States)

Despite decades of widespread use, the adverse effect profile of "selective" serotonin reuptake inhibitor (SSRI) antidepressants has still not been fully elucidated. Studies in male animals have shown delayed sexual development and reduced fertility. Three prospective cohort studies conducted in over one hundred patients exposed to an SSRI for periods ranging from 5 weeks to 24 months found altered semen param-eters after as little as 3 months of exposure: reduced sperm concentration, reduced sperm motility, a higher percentage of abnormal spermatozoa, and increased levels of sperm DNA fragmentation. One clinical trial showed growth retardation in children considered depressed who were exposed to SSRls. SSRls may have endocrine disrupting properties. Dapoxetine is a short-acting serotonin reuptake inhibitor that is chemically related to fluoxetine and marketed in the European Union for men complaining of premature ejaculation. But the corresponding European summary of product characteristics does not mention any effects on fertility. In practice, based on the data available as of mid-2014, the effects of SSRI exposure on male fertility are unclear. However, it is a risk that should be taken into account and pointed out to male patients who would like to father a child or who are experiencing fertility problems. PMID:25729824

2015-01-01

108

Characterization and usage of sexed semen from US field data  

Science.gov (United States)

The objectives were to characterize sexed semen available and its usage from US field data. This included investigating active Holstein proven bulls with sexed semen available, as well as percentages and frequencies of sexed semen matings for heifers and cows. Herds were also characterized for the...

109

Computer-assisted semen analysis and its utility for profiling boar semen samples.  

Science.gov (United States)

Achieving and maintaining a successful swine AI program depends on a number of factors, including accurate semen evaluation, typically sperm motility, morphology and concentration. Computer-Assisted Semen Analysis or CASA (i.e., image analysis with a phase-contrast microscope and computer measurements of motion parameters) objectively evaluates sperm motion characteristics, morphology and concentration. A total of 3077 semen collections were evaluated with CASA (on the day of collection), and a semen dose subset was used for single-sire AI of 6266 females over 6 months. Fertility data from these inseminations were fitted with models including farm/stud, line, boar, parity, mating week, semen age at mating and boar age at mating. The residuals from these models showed no correlation for any CASA semen unique motion parameter, which could be due to the level of sperm concentration, the number of inseminations per estrus, and the low number of females mated per boar. Future studies to expand CASA/fertility analysis need to address these constraints and may include analysis of extended boar semen after storage for 1 week. PMID:18723215

Didion, B A

2008-11-01

110

Beltsville poultry semen extender. 9. Effect of storage temperature on turkey semen held eighteen hours.  

Science.gov (United States)

Turkey semen was collected, diluted 1:1 with Beltsville Poultry Semen Extender, and held for 0 or 18 hr at 5, 15, 25, or 35 C. Changes in spermatozoa motility and sperm numbers were monitored before and after holding. All hens were artificially inseminated (AI) with 250 X 10(6) spermatozoa three times the first week and once weekly thereafter for a total of 20 weeks. No significant differences were observed in candling fertility (85 vs. 82%) of hens AI with unstored semen or semen held at 5 C for 18 hr. Significant depression of fertility levels to 41 and 40% were noted in hens AI with semen stored at 15 and 25 C, respectively. No fertile eggs were obtained from hens AI with semen held at 35 C for 18 hr. Sperm motility scores were not different between the unstored controls and samples held at 5 C (62 vs. 64%). Samples held at 15 and 25 C had motility scores of 40 and 8%, respectively. Samples held at 35 C for 18 hr were immotile. As semen holding temperature increased from 5 to 35 C, sperm numbers decreased during the 18 hr holding period by 11, 16, 28, and 45% of the unstored control. The decrease in sperm numbers during the 18-hr holding period was speculated to be the result of sperm aging which was compounded by sample agitation during storage. The methodology used for determining sperm numbers did not adversely influence the results. PMID:6622369

Giesen, A F; Sexton, T J

1983-07-01

111

Recognition of FT-IR Data Cuscutae Semen, Japanese Dodder, and Sinapis Semen Using Discrete Wavelet Transformation and RBF Networks  

OpenAIRE

Horizontal attenuation total reflection Fourier transformation infrared spectroscopy (HATR-FT-IR) studies on cuscutae semen and its confusable varieties Japanese dodder and sinapis semen combined with discrete wavelet transformation (DWT) and radial basis function (RBF) neural networks have been conducted in order to classify them. DWT is used to decompose the FT-IRs of cuscutae semen, Japanese dodder, and sinapis semen. Two main scales are selected as the feature extracting space in the DWT ...

Tao Hu; Xuexiang Weng; Lishan Xu; Cungui Cheng; Peng Yu

2013-01-01

112

Semen quality in Peruvian pesticide applicators: association between urinary organophosphate metabolites and semen parameters  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Organophosphates are broad class of chemicals widely used as pesticides throughout the world. We performed a cross-sectional study of associations between dialkylphosphate metabolites of organophosphates and semen quality among pesticide applicators in Majes (Arequipa, Peru. Methods Thirty-one men exposed to organophosphate (OP pesticides and 31 non-exposed were recruited (age, 20–60 years. In exposed subjects, semen and a blood sample were obtained one day after the last pesticide application. Subjects were grouped according to levels of OP metabolites in urine. Semen samples were analyzed for sperm concentration, percentage of sperm motility, percentage of normal morphology, semen leucocytes and concentrations of fructose and zinc. Exposure to OP was assessed by measuring six urinary OP metabolites (dimethyl and diethyl phosphates and thiophosphates by gas chromatography using a single flame photometric detector. Results Diethyldithiophosphate (p = 0.04 and diethylthiophosphate (p = 0.02 better reflected occupational pesticide exposure than other OP metabolites. Semen analysis revealed a significant reduction of semen volume and an increase in semen pH in men with OP metabolites. Multiple regression analysis showed that both occupational exposure to pesticides and the time of exposure to pesticides were more closely related to alterations in semen quality parameters than the single measurement of OP metabolites in urine. Conclusion The study demonstrated that occupational exposure to OP pesticides was more closely related to alterations in semen quality than a single measurement of urine OP metabolites. Current measurement of OP metabolites in urine may not reflect the full risk.

Gasco Manuel

2008-11-01

113

A dot-blot-immunoassay for semen identification using a polyclonal antibody against semenogelin, a powerful seminal marker.  

Science.gov (United States)

Among various seminal plasma proteins, semenogelin (Sg), produced in the seminal vesicle, has been considered a candidate for demonstrating the presence of semen. Sg consists of two proteins, one 52 kDa (Sg-I) in size, and the other a mixture of 71 and 76 kDa proteins (Sg-II). Recombinant Sg-I and Sg-II proteins were obtained using a baculovirus system and then injected into a rabbit to produce the respective antibodies [Characterization of recombinant precursor proteins of the human seminal plasma sperm motility inhibitor synthesized in insect cells, Int. J. Mol. Med. 2 (1998) 693]. When liquefied seminal plasma was immunoblotted with the anti-Sg-I and Sg-II antibodies, the anti-Sg-II antibody identified a wider range of the polypeptides originating from Sg than did the anti-Sg-I antibody. A dot-blot-immunoassay using anti-Sg-II antibody revealed a clear immunoreactive spot even when the semen was diluted 6400-fold. However, this assay showed that the Sg antigen was undetectable in saliva, urine, vaginal secretions, sweat, nasal secretions and serum. To determine the stability of Sg antigenic activity, filter paper with dried semen stains were kept at 37, 4 and 22 degrees C for 1, 6 and 18 months, respectively, and the Sg antigenic activity was examined. The activity was detectable in an area not less than 0.5 cm x 0.5 cm under all of the above environmental conditions during each period. Finally, semen was mixed with saliva or blood at various volumetric ratios, and used as a source of dried stains. The Sg antigenic activity was detectable in the stains until the ratio of semen to saliva or blood reached 1:8. These results suggest that Sg may be useful as a marker for semen identification. PMID:11587862

Sato, I; Yoshiike, M; Yamasaki, T; Yoshida, K; Takano, S; Mukai, T; Iwamoto, T

2001-10-15

114

Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen.  

Science.gov (United States)

The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and biochemical profiles such as cholesterol efflux and malondialdehyde (MDA) production. A total of 50 ejaculates were collected twice a week from 8 mithun bulls, and semen was split into 4 equal aliquots and diluted with the TEYC extender. Group 1: semen was without additives (control); groups 2 to 4: semen was diluted with 25?mM, 50?mM, and 100?mM of taurine, respectively. Seminal parameters and enzymatic and biochemical profiles were assessed at 5°C. Inclusion of taurine into diluent resulted in significant (P < 0.05) decreases in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities after liquid storage compared with the control group. Additionally, taurine at 50?mM has significant improvement in quality of mithun semen than taurine at 25 or 100?mM stored in in vitro at 5°C. It was concluded that the possible protective effects of taurine on sperm parameters are from enhancing the function of antioxidant enzymes, preventing efflux of cholesterol from cell membranes and decreased MDA production. PMID:23853737

Perumal, P; Vupru, Kezhavituo; Rajkhowa, C

2013-01-01

115

Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen  

OpenAIRE

The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and biochemical profiles such as cholesterol efflux and malondialdehyde (MDA) production. A total of 50 ejaculates were collected twice a week from 8 mithun bulls, and semen was split into 4 equal aliqu...

Perumal, P.; Kezhavituo Vupru; Rajkhowa, C.

2013-01-01

116

Review on international trade with boar semen.  

Science.gov (United States)

For more than 40 years, AI (artificial insemination) has been carried out with pigs. In some countries, it constitutes since the 1990 s the dominant procedure with piglet production to fertilize the sow. This procedure of insemination with fresh semen has become prevalent in all countries on a worldwide basis with an important pig meat production, with the exception of China. Meanwhile, up to 90% of the sows have been artificially inseminated. The trend is still upwards. As the need of pig meat and thus the production continues to increase, one can proceed on the assumption that the number of semen doses, which is necessary for this procedure, will likewise increase correspondingly. Until now, the trade beyond borders has only been marginal. An improvement in the sense of a longer shelf life for semen doses is indispensable for the trade over longer distances. PMID:21884268

Riesenbeck, A

2011-09-01

117

Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation.  

Science.gov (United States)

The variety of mammalian cells has been successfully cryopreserved by use of the silk protein sericin due to its strong free-radical-scavenging and potent antioxidant activity. The present study was conducted to examine the protective role of sericin on buffalo spermatozoa during cryopreservation. Semen of four breeding bulls was collected twice a week using artificial vagina technique. The ejaculates of four bulls were pooled, divided into five equal fractions, diluted with the extender supplemented with different concentrations of sericin (0, 0.25, 0.5, 1.5 and 2%) and then cryopreserved. Post-thawed motility was objectively assessed by computer assisted sperm analyzer. Sperm plasma membrane integrity was assessed by hypo-osmotic swelling test (HOST). Malondialdehyde (MDA) concentration, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in frozen-thawed extended seminal plasma by spectrophotometry. The extender supplemented with 0.25, 0.5 and 1% sericin resulted in the higher sperm motility and GPx acivity. Furthermore, plasma membrane integrity and SOD activity were found to be higher (Psericin (Psericin treated groups than control and other treated groups. In conclusion, the supplementation of 0.25-0.5% sericin in semen extender improves frozen-thawed semen quality through protecting sperm from oxidative stress. PMID:25497424

Kumar, Pradeep; Kumar, Dharmendra; Sikka, P; Singh, P

2015-01-01

118

Adenosine monophosphate-activated kinase, AMPK, is involved in the maintenance of the quality of extended boar semen during long-term storage.  

Science.gov (United States)

Boar semen preservation for later use in artificial insemination is performed by diluting semen in an appropriate medium and then lowering the temperature to decrease spermatozoa metabolism. The adenosine monophosphate-activated kinase, AMPK, is a key cell energy sensor that controls cell metabolism and recently has been identified in boar spermatozoa. Our aim was to investigate the role of AMPK in spermatozoa functional parameters including motility, mitochondrial membrane potential, plasma membrane integrity, acrosome integrity, and cell viability during long-term boar semen storage at 17 °C in Beltsville thawing solution. Boar seminal doses were diluted in Beltsville thawing solution in the presence or absence of different concentrations of AMPK inhibitor, compound C (1, 10, and 30 ?M) and evaluations were performed at 1, 2, 4, 7, or 10 days. Data demonstrate that AMPK becomes phosphorylated at threonine(172) (active) during storage of boar semen reaching maximum levels at Day 7. Moreover, AMPK inhibition during boar semen storage causes: (1) a potent inhibition of spermatozoa motility; (2) a reduction in the percentage of spermatozoa showing high mitochondria membrane potential; (3) a rise in the percentage of spermatozoa displaying high plasma membrane scrambling; and (4) a loss of acrosomal membrane integrity. Our study suggests that AMPK activity plays an important role in the maintenance of the spermatozoa quality during long-term storage of boar semen. PMID:23773688

Martin-Hidalgo, David; Hurtado de Llera, Ana; Yeste, Marc; Cruz Gil, M; Bragado, M Julia; Garcia-Marin, Luis J

2013-09-01

119

Seasonal Variations in Semen Characteristics in Arabic Rams  

OpenAIRE

The aim of this study was to evaluate the seasonal variations in semen characteristics in Arabic rams. 8 adult Arabic rams at the age of 2-3 years were used for this research. Semen was collected with electro ejaculator every 15 days for a period of 6 months (3 months of in breeding season and 3 months of non breeding season). Semen samples of these rams were subjected to the parameters including semen volume, sperm motility, sperm concentration, abnormal and live sperm percentage. Semen qual...

A Hamidi, M. Mamoei

2012-01-01

120

Inseminación artificial a tiempo fijo con semen ovino refrigerado / Timed artificial insemination with ram chilled semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Se evaluó la preñez resultante de la inseminación artificial sistemática cervical (IASC) con semen ovino refrigerado a 5ºC durante 12 o 24 h y dosis de 150 o 300 millones de espermatozoides. Doscientas ovejas adultas Merino se dividieron al azar en grupos de 40 animales, según arreglo factorial de l [...] os tratamientos (2x2) más un grupo control. En la estación reproductiva, los estros fueron sincronizados mediante 14 días con esponjas intravaginales con 60 mg acetato de medroxiprogesterona y 200 UI de eCG al retirar las esponjas. A las 12 y 24 h previas a la IASC se colectaron, diluyeron y refrigeraron los eyaculados. La dilución del semen se realizó con OviPro (Minitüb®, Alemania) en una relación 1:2 (semen/ diluyente). El grupo control fue inseminado con semen fresco sin diluir y dosis de 100 millones de espermatozoides. La IASC se realizó en el orificio uterino externo a las 54-56 h después del tratamiento progestacional. La preservación seminal durante 12 h alcanzó el 25% (10/40) y 38% (15/ 39) de preñez con dosis de 150 y 300 millones de espermatozoides. El semen preservado durante 24 h determinó el 3% (1/37) y 19% (7/37) de preñez con dosis inseminantes de 150 y 300 millones de espermatozoides, respectivamente. El porcentaje de preñez del grupo control (59%) evidenció que las condiciones de la majada no estuvieron afectadas por el estado nutricional o de manejo. La IASC con semen refrigerado ovino durante 12 h y una dosis de inseminación de 300 millones de espermatozoides, permitió obtener una preñez aceptable (38%) considerando el beneficio de poder transportar semen a largas distancias y su bajo costo operativo. Abstract in english We evaluated pregnancy by timed artificial insemination (TAI) with ram semen chilled at 5ºC during 12 or 24 h and insemination doses of 150 or 300 millions spermatozoa. Two hundred adult Merino sheep were randomly divided in 4 groups of 40 animals each, according to a factorial arrangement (2x2) plu [...] s a control group. During the breeding season, estrus were synchronized with intravaginal sponges impregnated with 60 mg of medroxyprogesterone acetate inserted for 14 days and administration of 200 UI PMSG at sponge removal. Twelve and 24 h before insemination, semen from adult Merino rams was collected, and after the ejaculates were diluted and chilled. Semen was diluted with the Ovipro extender (Minitüb®, Alemania) using a dilution rate of 1:2 (semen/extender). Control group was inseminated with fresh semen without diluent and an insemination dose of 100 millions spermatozoa. For every group, cervical TAI was performed 54-56 hours after progestational treatment. Preserved semen during 12 hours obtained 25% and 38% pregnancy with an insemination dose of 150 and 300 millions spermatozoa. Semen preserved for 24 hours caused 3% and 19% pregnancy with an insemination dose of 150 and 300 millions spermatozoa respectively. Control group showed a pregnancy of 59%, which evidenced that flock fertility was not affected by nutritional status or management. TAI with ram chilled semen during 12 h, with an insemination dose of 300 millions spermatozoa, was found to provide an acceptable fertility (38%), considering the benefit of carryng semen for long distances and the low operative cost for its implementation.

P., Naim; M., Cueto; A., Gibbons.

2009-09-01

121

IL-18 levels in the semen of male infertility: semen analysis.  

Science.gov (United States)

This study investigated the relationship between interleukin (IL)-18 levels and male infertility. Semen samples from 57 infertile and normal males were subjected to semen analysis. The pH of the semen samples from normal and abnormal groups showed no significant difference (p>0.05). However, semen volume, density, forward movement percentage, activity, survival rate, and normal morphology rate of the sperms in the normal group were significantly higher than those of the abnormal group (pseminal fluid IL-18 positively correlates with IL-6, IL-8, and tumor necrosis factor-?. Seminal IL-18 level does not affect bacterial colony count. Results indicate that IL-18 may be important in male reproduction. PMID:24333229

Qian, Li; Zhou, Yan; Du, Chunling; Wen, Jiangtao; Teng, Shijie; Teng, Zhaolin

2014-03-01

122

The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC  

Directory of Open Access Journals (Sweden)

Full Text Available The natural polyphenol resveratrol may be beneficial to many aspects of cell function and animal health, although its actions in the male reproductive system vary depending on animal species. This work investigates resveratrol effects on the quality of preserved boar semen during liquid storage at 17ºC. We used three approaches: 1 evaluation of conventional parameters of seminal quality, 2 measurement of specific response to capacitating stimuli, and 3 evaluation of mitochondria membrane potential and ATP content. Resveratrol supplementation causes i a loss in the response of liquid stored boar spermatozoa to capacitating stimuli, ii a decrease in the sperm ATP content and iii a reduction in the mitochondrial membrane potential. Moreover, higher concentrations of resveratrol increase plasma membrane phospholipid disorder and reduce the percentage of motile spermatozoa. These results suggest that semen doses supplemented with resveratrol could be considered sub-fertile compared with semen stored hypothermically in standard conditions.

David Martín-Hidalgo

2013-07-01

123

Semen analysis: a new manual and its application to the understanding of semen and its pathology  

OpenAIRE

This article reviews the latest edition of the World Health Organization's manual on semen analysis, a comprehensive instructional guide. The methodology used in the assessment of the usual variables in semen analysis is described, as are many of the less common, but very valuable, sperm function tests. Seminal fluid preparation techniques for procedures such as in vitro fertilization and intrauterine insemination are also outlined in the manual. In addition, it details many useful techniques...

Jequier, Anne M.

2010-01-01

124

Effect of insulin-like growth factor-I on some quality traits and fertility of cryopreserved ovine semen.  

Science.gov (United States)

The objective was to evaluate the effects of insulin-like growth factor-I (IGF-I) on the quality and fertility of frozen/thawed ovine semen. Five rams (five ejaculates/ram) were used for evaluation of semen parameters. Before cryopreservation, ejaculates were divided into four aliquots and extended with Tris alone or supplemented with human IGF-I (50, 100, or 250 ng/mL). Semen was evaluated immediately after thawing (T0), after 1 h (T1) and 2 h (T2) post-incubation at 37 °C. The percentage of live cells (fluorescence analysis-calcein and ethidium), acrosome integrity (NAR) and motility were analyzed, and hypo-osmotic swelling tests (HOST) were used to evaluate membrane resistance. In addition, AI was performed using 121 ewes to compare the optimal concentration of IGF-I vs. Tris alone on pregnancy rates after laparoscopic insemination. Pregnancy diagnosis was performed by transrectal ultrasonography. After 1 and 2 h post-incubation, in every group, percentage motile sperm, NAR and HOST decreased compared to semen at T0. Motility was higher (P 0.05) in NAR or hypo-osmotic swelling tests (HOST) among groups. There were no differences (P > 0.05) in fertility between the IGF-I 100 and Tris groups. In conclusion, IGF-I improved subjective sperm motility and structural integrity of the plasma membrane without a significant effect on 45-day pregnancy rates after laparoscopic insemination of ewes with frozen-thawed semen. PMID:22541171

Padilha, R T; Magalhães-Padilha, D M; Cavalcante, M M; Almeida, A P; Haag, K T; Gastal, M O; Nunes, J F; Rodrigues, A P R; Figueiredo, J R; Oliveira, M A L

2012-09-01

125

AIR POLLUTION EFFECTS ON SEMEN QUALITY  

Science.gov (United States)

The potential impact of exposure to periods of high air pollution on male reproductive health was examined within the framework of an international project conducted in the Czech Republic. Semen quality was evaluated in young men (age 18) living in the Teplice District who are ex...

126

Semen collection in cats: techniques and analysis.  

Science.gov (United States)

Sperm collection permits to obtain material for artificial insemination, diagnostic purposes, and use in research. Collections via artificial vagina (AV) and electroejaculation (EE) are the most commonly used in the cat and permit to obtain ejaculates with good quality, but EE seems to be the method of choice. Other methods of semen collection, such as epididymal squeezing or slicing, to achieve material for research have been reported. Semen analysis is essential for the fertility evaluation of male felids, but some of the diagnostic procedures used in other species are difficult or impossible to duplicate, due to the small volume of the cat ejaculate. In practice, sperm motility and viability (using eosin-nigrosin staining), morphology and sperm concentration should be evaluated, even if determination of sperm membrane and acrosomal integrity (using transmission electromicroscopy or fluorescence microscopy), semen chemistries and other parameters can be important for testing both fresh and frozen-thawed sperm. This manuscript reviews the techniques for semen collection and analysis. PMID:16527340

Zambelli, Daniele; Cunto, Marco

2006-07-15

127

IMPACT OF FENBENDAZOLE ON TURKEY SEMEN QUALITY  

Science.gov (United States)

Fenbendazole (FBZ) is an anthelmintic recently approved to treat and control nematode infections in growing turkeys. When administered to growing turkeys there are no detrimental side effects. However, when we used FBZ to treat nematodes in mature breeder toms, we observed a decrease in semen quali...

128

Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender.  

Science.gov (United States)

The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin-based semen extender as a substitute for egg yolk-based extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different extenders: (i) Tris-based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT extenders. The results showed that the proportion of live post-thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT extenders. In addition, SL1, SL1.5 and SL2.5 extenders resulted in significantly lower percentage of early-apoptotic sperm than that in EYT extender. There were no significant differences in different semen extenders for percentage of post-thawed necrotic and late-apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between extenders. In conclusion, SL1.5 extender was better than other extenders in most in vitro evaluated sperm parameters. PMID:23701189

Emamverdi, M; Zhandi, M; Zare Shahneh, A; Sharafi, M; Akbari-Sharif, A

2013-12-01

129

Developmental validation of RSID™-Semen: a lateral flow immunochromatographic strip test for the forensic detection of human semen.  

Science.gov (United States)

Tests for the identification of semen commonly involve the microscopic visualization of spermatozoa or assays for the presence of seminal markers such as acid phosphatase (AP) or prostate-specific antigen (PSA). Here, we describe the rapid stain identification kit for the identification of semen (RSID™-Semen), a lateral flow immunochromatographic strip test that uses two antihuman semenogelin monoclonal antibodies to detect the presence of semenogelin. The RSID™-Semen strip is specific for human semen, detecting <2.5 nL of semen, and does not cross-react with other human or nonhuman tissues tested. RSID™-Semen is more sensitive with certain forensic evidence samples containing mixtures of vaginal secretions and semen than either of the commercially available PSA-based forensic semen detection tests or tests that measure AP activity that were tested in parallel. The RSID™-Semen kit also allows sampling a fraction of a questioned stain while retaining the majority of the sample for further processing through short tandem repeat analysis. PMID:22211796

Old, Jennifer; Schweers, Brett A; Boonlayangoor, Pravat W; Fischer, Brian; Miller, Kevin W P; Reich, Karl

2012-03-01

130

9 CFR 98.33 - Ports designated for the importation of certain animal semen.  

Science.gov (United States)

...Ports designated for the importation of certain animal semen. 98.33 Section 98.33 Animals and Animal... IMPORTATION OF CERTAIN ANIMAL EMBRYOS AND ANIMAL SEMEN Certain Animal Semen § 98.33 Ports designated for the...

2010-01-01

131

9 CFR 98.35 - Declaration, health certificate, and other documents for animal semen.  

Science.gov (United States)

...health certificate, and other documents for animal semen. 98.35 Section 98.35 Animals and Animal... IMPORTATION OF CERTAIN ANIMAL EMBRYOS AND ANIMAL SEMEN Certain Animal Semen § 98.35 Declaration, health certificate,...

2010-01-01

132

Improving outcome of in vitro sperm activation using non–liquefied versus liquefied semen of oligoasthenozoospermic patients  

Directory of Open Access Journals (Sweden)

Full Text Available Objective: To evaluate the efficacy of in vitro sperm activation (ISA using non-liquefied versus liquefied asthenozoospermic semen samples for improvement of sperm parameters. "nMaterials and Methods: Fifty six oligoasthenozoospermic (OA patients (age range: 22-44 years; mean: 32.089 years were enrolled in this study. OA patients were classified according to type of infertility. Also, duration of infertility was recorded. Fifty six semen samples were collected, and seminal fluid analyses were done involving macroscopic and microscopic examinations were performed according to WHO methodology. Direct swim–up technique was used to separate the motile spermatozoa from seminal plasma. Minimum Essential Medium Eagle (MEME enriched with 5% Human serum albumin (HSA was used. One mL of either non–liquefied or liquefied semen was layered beneath 1 mL of MEME enriched with 5% HSA, and placed for incubation in an air incubator at 37 oC for 30 minutes. Then, one drop (10 ?L from upper layer of culture medium was taken using automatic pipette to be examined under high power field (40 X for assessment of sperm parameters."nResults: According to type of infertility, infertile patients were classified into patients with either primary infertility (no. 46; 82.15 % or secondary infertility (no. 10; 17.85 %. In contrast to other parameters, significant (P<0.05 reductions were noticed in the percentages of sperm motility and progressive sperm motility for OA patients with primary infertility as compared to OA patients with secondary infertility. All sperm parameters were significantly (P<0.001 enhanced after in vitro activation of liquefied and non-liquefied semen samples when compared to pre-activation. In the present study, best results were achieved for non-liquefied semen samples as compared to liquefied semen samples."nConclusion: It was concluded that the outcome of ISA was enhanced in regard to sperm parameters when using non-liquefied semen of OA patients. Furthermore, some components of seminal plasma of OA patients may be have harmful effects on certain sperm functions when in vitro incubated for longer periods. Further study is recommended to investigate the effect of in vitro sperm activation from non-liquefied semen on successful rate of artificial insemination husband.

Muhammad Baqir Fakhrildin

2010-01-01

133

Optimalization of Equilibration and Thawing Methode on Freezing Process of Garut Ram Semen (Ovis aries  

Directory of Open Access Journals (Sweden)

Full Text Available Generally, the problem of semen freezing process is cold shock effect and intracelullar change due to water release which is related to ice crystal formation. One factor to solve the problem is finding out optimal equilibration time and thawing method so there is only a little damage spermatozoa during freezing process. The research has been done to explore the influence of equilibration time and thawing methode on frozen semen quality of garut rams. The results shown that the mean of percentages of progressive motile sperm, percentages of viable sperm, percentages of plasma membrane and acrosomal intact on four hours equilibration (52.50% ; 62.33%; 57.17% and 56.42% were not significantly different (P>0.05 from five hours equilibration (52.27%; 63.67%; 56.92% and 57.58 % and six hours equilibration (54.17%; 61.00%; 59.42% and 58.58% respectively. The percentages of progressive motile sperm, percentages of viable sperm, percentages of plasma membrane and acrosomal intact on the thawing method on the temperature of 370C for 30 seconds (53.33% ; 62.39%; 57.94% dan 58.61 were not significantly different (P>0.05 from the thawing methode on the temperature of 250C for 45 seconds (52.22% ; 62.28%; 57.72% dan 56.44 respectively. The conclusion shown that the treatment of four hours equilibration, five hours equilibration, six hours equilibration and also the treatment of thawing method on the temperature of 370C for 30 seconds and the thawing methode on the temperature of 250C for 45 seconds do not have effect on garut ram freezing semen quality. (Animal Production 7(2: 74-80 (2005Key Words : Equilibration, Thawing, Semen, Garut ram

Herdis

2005-05-01

134

Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen.  

Science.gov (United States)

Semenogelin (Sg), a protein originating in the seminal vesicles and a substrate for prostate specific antigen (PSA or p30), is a useful marker for the identification of semen. And detection of Sg has been available commercially in a membrane test recently. PSA is commonly used to detect semen in forensic significant samples taken from sexual assault cases. The strip PSA test has been available commercially from various manufacturers for many years. In this study, we evaluated two immunochromatographic membrane tests, one for Sg and the other for PSA by analyzing human semen, other human bodily fluids/materials including urine, blood, saliva, sweat, breast milk, vaginal secretion and fecal materials, semen from various animals and forensic casework samples. The data demonstrate that both Sg and PSA strip tests provide rapid and sensitive method for identification of seminal plasma. These results show that the immunochromatographic method for Sg detection is useful for the identification of seminal plasma in forensic samples, an alternative to the method for PSA detection. PMID:16949235

Pang, B C M; Cheung, B K K

2007-06-14

135

Comparison of biochemical parameters of Muscovy drake semen diluted and stored at 4 degrees C in three buffers.  

Science.gov (United States)

A comparison study of biochemical parameters of semen from Muscovy drakes diluted and stored at 4 degrees C in three buffers--IMV-buffer (France), HIA-1 and AU (Bulgaria) was carried out. The ejaculates were collected twice a week from ten 1-year-old Muscovy drakes using laying Muscovy females as teaser. Semen was diluted immediately, respectively, with IMV-buffer, HIA-1 and AU, and cold-stored (4 degrees C) for 1, 3 and 6h. The intensities of oxygen uptake at the third hour in semen diluted, respectively, with IMV-buffer (200+/-1.6 nAO/10(9) spermcellsmin), with HIA (224+/-44 nAO/10(9) spermcellsmin) and with AU (238+/-48 nAO/10(9) spermcellsmin) were highly significant in comparison with neat semen (75+/-0.7 nAO/10(9)spermcellsmin). The observed intensity of fructolysis was highest when using AU, followed by HIA-1 and IMV-buffer. During the first hour of storage the level of pyruvic acid was significantly lower in semen diluted with Bulgarian extenders, and this stability for AU referred to the entire period. For lactic acid, the differences were not statistical significant. Our investigations do not show significant differences concerning the dynamics of inorganic phosphate and total lipids after dilution with all tested extenders. On the contrary, high increase of cholesterol efflux from spermatozoa to seminal plasma-diluents were obtained after 6 h of storage. All extenders, IMV-buffer (France), HIA-1 and AU (Bulgaria) for diluting and short time storage of semen from Muscovy drakes at 4 degrees C maintain the necessary comfort of energy metabolism of the spermatozoa. PMID:16971072

Atanasov, V; Gerzilov, V; Dyshlianova, E

2007-08-01

136

Bivalent response to long-term storage in liquid-preserved boar semen: a flow cytometric analysis.  

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The fertility of liquid-preserved boar semen declines during storage at 17°C, insemination trials even indicating early losses in fertilizing ability within the first 24-48 h of storage. Standard semen parameters barely reflect these changes in semen quality, and new approaches for assessment of functional changes in stored spermatozoa are needed. Capacitation, the essential prefertilization step for spermatozoa in the female genital tract, is specifically induced in vitro by bicarbonate. Therefore, we have investigated changes in responsiveness of boar spermatozoa to bicarbonate during storage. Ejaculates of 14 boars were diluted in Beltsville thawing solution, cooled to 17°C and stored for 12, 24, 72, 120, and 168 h before investigation. At each time, basic semen quality was characterized by sperm motility and viability. Subsequently, washed subsamples were incubated in variants of an in vitro fertilization (IVF) medium and assessed for kinetic changes of viability (plasma membrane integrity) and intracellular calcium concentration using flow cytometry in combination with propidium iodide and Fluo-3. By this means, it was possible to determine specific effects of bicarbonate and calcium on sperm subpopulations over incubation time. During storage, standard semen parameters remained on a high level. However, flow cytometric analysis of sperm responses to capacitating and control media revealed two opposing effects of storage. There was a loss of response to bicarbonate in part of the live sperm population but an increasing degree of instability in the rest. Assessment of response to capacitating media by flow cytometry appears a markedly more sensitive way of monitoring sperm functionality during storage than the standard semen parameters of motility and viability. PMID:22573481

Henning, Heiko; Petrunkina, Anna M; Harrison, Robin A P; Waberski, Dagmar

2012-07-01

137

Differences in the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques.  

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The present study aimed to evaluate the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques. Eighteen sperm-rich ejaculate samples from six boars (three per boar) were diluted in Beltsville Thawing Solution and split into three aliquots. The aliquots were (1) further diluted to 3×10(7) sperm/mL and stored as a liquid at 17°C for 72 h, (2) frozen-thawed (FT) at 1×10(9) sperm/mL using standard 0.5-mL straw protocols, or (3) sex-sorted with subsequent liquid storage (at 17°C for 6 h) or FT (2×10(7) sperm/mL using a standard 0.25-mL straw protocol). The sperm quality was evaluated based on total sperm motility (the CASA system), viability (plasma membrane integrity assessed using flow cytometry and the LIVE/DEAD Sperm Viability Kit), lipid peroxidation (assessed via indirect measurement of the generation of malondialdehyde (MDA) using the BIOXYTECH MDA-586 Assay Kit) and DNA fragmentation (sperm chromatin dispersion assessed using the Sperm-Sus-Halomax(®) test). Data were normalized to the values assessed for the fresh (for liquid-stored and FT samples) or the sorted semen samples (for liquid stored and the FT sorted spermatozoa). All of the four sperm-processing techniques affected sperm quality (Psemen donor, with reduced percentages of motile and viable sperm and increased MDA generation and percentages of sperm with fragmented DNA. Significant (Pboar (effect of boars within each semen-processing technique) and intra-boar (effect of semen-processing techniques within each boar) differences were evident for all of the sperm quality parameters assessed, indicating differences in the ability of spermatozoa from individual boars to withstand the semen-processing techniques. These results are the first evidence that ejaculate spermatozoa from individual boars can respond in a boar-dependent manner to different semen-processing techniques. PMID:22554791

Parrilla, Inma; del Olmo, David; Sijses, Laurien; Martinez-Alborcia, María J; Cuello, Cristina; Vazquez, Juan M; Martinez, Emilio A; Roca, Jordi

2012-05-01

138

Semen quality of Italian local pig breeds  

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Full Text Available From 1996 to 1999 a conservation programme was carried out within the framework of EC contract “European gene banking project for the pig genetic resources” (Ollivier et al., 2001 in the Italian local pig breeds. The aims of the program included the primary characterization of the breeds, i.e. information on the organization in charge of the breed, breeding population numbers, breed description and qualifications, and field trials on productive and reproductive performances. In this context the “Semen Bank of Italian local pig breeds” was built. A total of 30,835 straws of four Italian local pig breeds (Cinta Senese, Casertana, Mora Romagnola and Nero Siciliano, collected from 42 sires, have been stored. In this work semen quality traits, lipid composition and freezability of the four Italian local pig breeds are reported.

G. Gandini

2010-01-01

139

Random laser action in bovine semen  

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Experiments using bovine semen reveal that the addition of a high-gain water soluble dye results in random laser action when excited by a Q-switched, frequency doubled, Nd:Yag laser. The data shows that the linewidth collapse of the emission is correlated to the sperm count of the individual samples, potentially making this a rapid, low sample volume approach to count determination.

Smuk, Andrei; Lazaro, Edgar; Olson, Leif P.; Lawandy, N. M.

2011-03-01

140

Relationship between Thyroid Profile and Semen Quality  

OpenAIRE

Background: Endogenous hormones are critical to spermatogenesis and maintenance of male reproductive function. Follicle stimulating hormone (FSH), luteinizing hormone (LH), inhibin B and testosterone all serve important and well-known functions in the male hypothalamopituitary-gonadal axis and male reproduction. However, the potential relationship between other hormones, including thyroid hormones, and semen quality are still not completely understood. Thus in the present study an attempt has...

Manoj Kumar Sharma, Deepak Parchwani

2012-01-01

141

Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen  

OpenAIRE

The objectives of the present study were to determine the effects of six different antibiotics in controlling the growth of semen contaminating bacteria and if these antibiotics have any adverse effect on Awassi ram spermatozoa. Semen samples from six mature Awassi rams were used in this study. A total number of 120 ejaculates were collected from the rams using an artificial vagina once a week. Semen ejaculates were evaluated for volume, sperm concentration, mass motility, individual motility...

Azawi, O. I.; Ismaeel, M. A.

2012-01-01

142

Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality  

OpenAIRE

Some previous studies have identified bacteria in semen as being a potential factor in male infertility. However, only few types of bacteria were taken into consideration while using PCR-based or culturing methods. Here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. Ninety-six semen samples collected were examined for bacterial communities, measuring seven ...

Weng, Shun-long; Chiu, Chih-min; Lin, Feng-mao; Huang, Wei-chih; Liang, Chao; Yang, Ting; Yang, Tzu-ling; Liu, Chia-yu; Wu, Wei-yun; Chang, Yi-an; Chang, Tzu-hao; Huang, Hsien-da

2014-01-01

143

Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality.  

Science.gov (United States)

In captivity, male Asian elephants often yield poor quality semen after transrectal manually assisted semen collection; however, the reasons for the disappointing semen quality are not clear. Here we test the hypothesis that accumulation of senescent spermatozoa is a contributory factor, and that semen quality can therefore be improved by more frequent ejaculation. To this end we investigated the effect of collecting semen five times on alternate days, after a long period of sexual rest, on semen quality in Asian elephants known to deliver poor semen during infrequent single collections. All eight bulls initially displayed a high incidence of detached sperm heads and low percentages of motile (close to 0%) spermatozoa. After semen collection on alternate days, the percentages of detached sperm heads, and head and mid-piece abnormalities, were reduced significantly (p<0.05). In particular, one bull showed markedly improved sperm motility (increased from 0% to 60%) and membrane integrity (increased from 5% to 75%). In addition, advancing age significantly (p<0.01) correlated with lower percentages of sperm with intact membranes and a higher frequency of detached sperm heads. In contrast to sperm accumulation problems in other species, a small ampullary diameter correlated significantly (p<0.05) with reduced semen quality. PMID:24832106

Imrat, P; Mahasawangkul, S; Thitaram, C; Suthanmapinanth, P; Kornkaewrat, K; Sombutputorn, P; Jansittiwate, S; Thongtip, N; Pinyopummin, A; Colenbrander, B; Holt, W V; Stout, T A E

2014-06-30

144

Criopreservação de sêmen suíno: avanços tecnológicos e perspectivas / Cryopreservation of boar semen: progress and perspectives / Criopreservación de semen de verraco: avances y perspectivas tecnológicas  

Scientific Electronic Library Online (English)

Full Text Available Resumo A criopreservação de sêmen suíno é uma técnica ainda não consolidada devido à alta sensibilidade do espermatozoide da espécie ao processo de congelamento e descongelamento. Ainda assim, a utilização do sêmen criopreservado é altamente desejável para o intercâmbio genético e manutenção da bios [...] segurança. Esta revisão tem como objetivo ressaltar alguns fatores limitantes do processo e apontar os consideráveis avanços desenvolvidos nos últimos anos, principalmente devido ao aperfeiçoamento das técnicas já existentes, como caracterização das proteínas do ejaculado, ajustes na remoção do plasma seminal e uso de adjuvantes na confecção dos diluentes. Todas estas técnicas tornarão a criopreservação do sêmen suíno mais aplicável nos próximos anos para que possa ser finalmente uma técnica de uso comercial. Abstract in spanish Resumen La criopreservación del semen de porcino es una técnica aún no consolidada debido a la alta sensibilidad del espermatozoide de esta especie al proceso de congelación y descongelación, aun así, el uso de semen criopreservado es altamente deseable para el intercambio genético y el mantenimient [...] o de la bioseguridad. Esta revisión tiene por objeto poner de relieve algunos factores limitantes del proceso y señalar las importantes avances desarrollados en los últimos años, debido principalmente al mejoramiento de las técnicas existentes, entre ellas, la caracterización de las proteínas de la eyaculación, los ajustes de extracción del plasma seminal y el uso de adyuvantes en la producción de los diluyentes. Todas estas técnicas harán que la criopreservación del semen de porcino sea más aplicable en los próximos años, para ser finalmente una técnica de uso comercial. Abstract in english Abstract Biotechnology of boar semen cryopreservation has not succeeded due to the high sensitivity of swine sperm to the freezing and thawing process. However, its use is highly desirable for genetic improvement and maintenance of biosecurity. This review aims to highlight some limitations of the p [...] rocess and point out important advances obtained in recent years, including the improvement of existing techniques, such as protein characterization of the ejaculate, adjustments in the removal of seminal plasma, and use of adjuvants in the manufacture of diluents; all of which will make cryopreservation commercially available in the near future.

Tainã, Figueiredo Cardoso; Estela, Fernandes e Silva; Carine, Dahl Corcini.

2013-07-01

145

Semen characteristics and malondialdehyde levels in men with different reproductive problems.  

Science.gov (United States)

The aim of this study was to assess the level of malondialdehyde (MDA) in the seminal plasma of infertile men and to highlight a relationship between the level of MDA and semen parameters. Eighty-one infertile patients were divided into groups according to their clinical diagnosis: genitourinary infections, varicocele and idiopathic infertility. Semen quality was assessed by light and transmission electron microscopy (TEM). TEM data were quantified with a mathematical formula able to obtain a fertility index and the percentage of sperm apoptosis, immaturity, and necrosis. Seminal MDA levels were determined by spectrofluorometry. Scrotal Eco-color Doppler was used to detect the varicocele. Infected patients had a positive bacteriological semen analysis. A control group consisted of 14 normospermic fertile men. Fertile group showed significantly increased values of sperm concentration, motility, and fertility index compared to infertile groups. In the infertile groups, sperm motility, concentration, apoptosis, and fertility index were not significantly different. In infection group, the percentage of necrosis was significantly higher than that observed in fertile men, varicocele, and idiopathic infertility groups (p fertile men (p fertility index (p fertile men and idiopathic infertility group, they did not show any correlation. In conclusion, we suggest that the evaluation of seminal MDA may be a good marker for understanding pathologies responsible of a sperm motility reduction such as urogenital infections or inflammatory status. PMID:25331426

Collodel, G; Moretti, E; Micheli, L; Menchiari, A; Moltoni, L; Cerretani, D

2014-10-20

146

Effects of re-infection with Neospora caninum in bulls on parasite detection in semen and blood and immunological responses.  

Science.gov (United States)

Three bulls with experimentally induced primary infection with Neospora caninum were re-infected intravenously with 10(8) live N. caninum tachyzoites of the NC-1 isolate at 300 days post-infection to investigate the presence of N. caninum in semen and blood, and the associated immune responses. In parallel, three bulls with experimentally induced primary infection with N. caninum and three non-infected bulls were also monitored. Re-infected and infected bulls showed an intermittent presence of N. caninum DNA in semen with a parasite load ranging from 0.1 to 15.6 (mean 4.4) and 0.1 to 11.1 (mean 4.1) parasites/ml, respectively. Re-infected bulls showed significant and persistent serum-specific IgM and IgG antibody responses. Specific IgG levels were detected in seminal plasma of all infected bulls, but the magnitude of the response was significantly higher in re-infected rather than in chronically infected animals. The mean specific IFN-gamma levels in re-infected bulls were significantly increased as early as 3 and 7 days after experimental infection when compared to bulls in other groups. This study showed that the intermittent presence and parasite load of N. caninum in the semen of re-infected bulls is very similar to that reported in chronically infected animals. The protozoa could not be isolated from BALB/c nu/nu mice inoculated with PCR-positive semen samples and inseminated heifers with pooled semen samples did not show seroconversion. Plasma IFN-gamma level seems to be a good indicator of a recent N. caninum infection. PMID:18336895

Ferre, I; Serrano-Martínez, E; Martínez, A; Osoro, K; Mateos-Sanz, A; Del-Pozo, I; Aduriz, G; Tamargo, C; Hidalgo, C O; Ortega-Mora, L M

2008-04-15

147

Cryopreservation of boar semen by egg yolk-based extenders containing lactose or fructose is better than sorbitol.  

Science.gov (United States)

The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (Pfructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen. PMID:21997240

Chanapiwat, Panida; Kaeoket, Kampon; Tummaruk, Padet

2012-03-01

148

Differences in preservation of canine chilled semen using different transport containers.  

Science.gov (United States)

In the present study, the effect of three different containers in the preservation of dog chilled semen, during 24, 48 and 72h was evaluated. Weekly sperm pools of different dogs were obtained, during 10 consecutive weeks. Semen samples were diluted in egg-yolk-Tris-fructose extender and stored in a Styrofoam box, a common Thermos flask and an Equitainer. Progressive motility, morphology and sperm membrane integrity were examined in semen aliquots taken daily from each container during the 3 days of storage. Additionally, integrity of the acrosome and sperm plasma membranes, determined by PI/Fitc-PSA staining was assessed at 48 and 72h of storage. At 24h no differences were observed between the three containers for the evaluated parameters. At 48h samples kept in the Equitainer presented a higher progressive motility than samples kept in the Thermos. At 72h, progressive motility was higher in the Equitainer than in the other two containers. Only samples kept in the Equitainer maintained similar levels of progressive motility between 24 and 72h. Membrane integrity assessed by eosin-nigrosin deteriorated over the 72h period, whereas functional membrane integrity determined by the hypoosmotic swelling test was independently affected by type of container (the Equitainer) kept a higher percentage of sperm cells with intact membrane) and time of storage (a decrease of membrane integrity between 24 to 72h). Staining with PI-Fitc-PSA allowed the detection of differences between containers but not between the two studied storage periods (48 and 72h). The results indicated that the use of the Equitainer is preferable when transporting chilled dog semen for more than 48h. PMID:18479849

Lopes, G; Simões, A; Ferreira, P; Martins-Bessa, A; Rocha, A

2009-05-01

149

Opportunities to improve liquid and frozen storage of boar semen  

Science.gov (United States)

Artificial insemination has facilitated the utilization of superior genetics, and has reduced boar biosecurity problems and housing costs. The use of frozen semen permits the flexibility to inseminate animals at unscheduled times and to use semen of deceased boars. While good long-term storage exten...

150

AVIAN SEMEN CRYOPRESERVATION: WHAT ARE THE BIOLOGICAL CHALLENGES?  

Science.gov (United States)

More than fifty years ago, the discovery of glycerol’s cryoprotective properties pioneered the success of modern cryobiology and led to the development of semen cryopreservation for a wide range of species. Despite the fact that this scientific breakthrough was accomplished with rooster semen, the ...

151

Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis semen  

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Full Text Available Aim: The experiment was conducted to study the total seminal plasma protein (TSPP and heparin-binding proteins (HBPs in relation to initial semen quality of buffalo bull. Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790 with mass motility of ?3+ were used for the study and categorized into three groups (Group I- Mass motility 3+, Group II- Mass motility 4+ and Group III- Mass motility 5+. Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry’s method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls. Results: The mean values of TSPP concentrations in bull no. 605 and 790 in Group I, II and III were 30.64±0.12, 31.66±0.09, 32.53±0.19 and 28.51±0.09, 29.49±0.15, 30.45±0.17 mg/mL, respectively. The mean values of HBPs concentrations in bull no. 605 and 790 in Group I, II and III were 3.11±0.07, 3.32±0.06, 3.46±0.08 and 2.51±0.08, 2.91±0.05, 3.10±0.03 mg/mL, respectively. Both the values of TSPP and HBPs were significantly higher (p<0.01 in bull no. 605 when compared to 790 in all the three groups. 31 kDa HBP was more intensely present in bull no. 605, thus may indicate its superiority over bull no. 790 in relation to fertility potential. Conclusion: TSPP and HBPs shows variation in concentration with respect to initial semen quality. Furthermore, presence of fertility related 31 kDa HBPs in one of the bull may be an indication of high fertility of a bull. In future, in-vivo and in-vitro correlative study on larger basis is needed for the establishment of fertility-related HBPs in semen which might establish criteria for selection of buffalo bull with high fertility potential.

S. S. Ramteke

2014-09-01

152

9 CFR 98.34 - Import permits for poultry semen and animal semen.  

Science.gov (United States)

...the Chief, Foreign Animal Disease Diagnostic Laboratory...veterinary inspection, and testing while the donor animal was on the farm of origin...importer, until all of the testing required to be conducted...indications that the donor animal or the semen from...

2010-01-01

153

[Variation trend of male fertility and semen parameters].  

Science.gov (United States)

In recent years, the variation trend of male fertility and semen parameters has aroused much academic controversy and become a focus of public attention. For the assessment of male fertility, female pregnancy is regarded as a gold standard, but semen parameters are commonly used as surrogate or indirect evidence in clinical practice and laboratory research. The reference range of se- men parameters being used in China is based on the WHO recommended data and lacks the specific reference value for healthy Chinese men. No definite conclusion has yet been derived from studies at home and abroad on the general variation trend of semen parameters worldwide, but many researchers agree on the decline of semen quality in some areas of the world. Long-term continuous prospective studies are needed for the evaluation and prediction of the general variation trend of semen quality. PMID:25597168

Gu, Yi-Qun

2014-12-01

154

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality  

Directory of Open Access Journals (Sweden)

Full Text Available It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress and 3 months after (non-stress the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10(6/mL, rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63% and seminal plasma arginase activity (0.12 ± 0.01 vs 0.22 ± 0.01 U/mL were significantly lower than in the non-stress situation, whereas seminal plasma NO (17.28 ± 0.56 vs 10.02 ± 0.49 µmol/L was higher compared to the non-stress period (P < 0.001 for all. During stress there was a negative correlation between NO concentration and sperm concentration, the percentage of rapid progressive motility and arginase activity (r = -0.622, P < 0.01; r = -0.425, P < 0.05 and r = -0.445, P < 0.05, respectively. These results indicate that psychological stress causes an increase of NO level and a decrease of arginase activity in the L-arginine-NO pathway. Furthermore, poor sperm quality may be due to excessive production of NO under psychological stress. In the light of these results, we suggest that the arginine-NO pathway, together with arginase and NO synthase, are involved in semen quality under stress conditions.

S. Eskiocak

2006-05-01

155

A successful new approach to honeybee semen cryopreservation.  

Science.gov (United States)

Honeybee biodiversity is under massive threat, and improved methods for gamete cryopreservation could be a precious tool for both the in situ- and ex situ-conservation of subspecies and ecotypes. Recent cryoprotocols for drone semen have improved the viability and fertility of frozen-thawed semen by using increased diluent:semen-ratios, but there is still much room for progress. As semen cryopreserved after dilution often appeared hyperactive, we speculated that the disruption of sperm-sperm interactions during dilution and cryopreservation could reduce the fertile lifespan of the cells. We therefore developed protocols to reduce admixture, or abolish it altogether by dialyzing semen against a hypertonic solution of cryoprotectant. Additionally, we tested methods to reduce the cryoprotectant concentration after thawing. Insemination of queens with semen cryopreserved after dialysis yielded 49%, 59% and 79% female (= stemming from fertilized eggs) pupae in three separate experiments, and the numbers of sperm found in the spermathecae of the queens were significantly higher than those previously reported. Post-thaw dilution and reconcentration of semen for cryoprotectant removal reduced fertility, but sizeable proportions of female brood were still produced. Workers stemming from cryopreserved semen did not differ from bees stemming from untreated semen with regard to indicators of fluctuating asymmetry, but were slightly heavier. Cryopreservation after dialysis tended to increase the proportion of cells with DNA-nicks, as measured by the TUNEL-assay, but this increase appears small when compared to the baseline variations of this indicator. Overall, we conclude that cryoprotectant-addition through dialysis can improve the quality of cryopreserved drone semen. Testing of offspring for vitality and genetic integrity should continue. PMID:25088062

Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

2014-10-01

156

Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Semen analysis is the corner stone of infertility evaluation as it provides information on the functional status of the seminiferous tubules, epididymis and accessory sex glands. The methods on how the human semen should be evaluated are provided by the World Health Organization, which periodically [...] releases manuals that include specific protocols and reference standards. In 2010, the WHO published new criteria for human semen characteristics that were markedly lower than those previously reported. In this review initially it is discussed the limitations of semen analysis as a surrogate measure of a man’s ability to father a pregnancy. Secondly, it is analyzed methodology issues that could explain why the newly released reference values were different from those earlier reported. Thirdly, it is speculated on the likely effects of the 2010 WHO criteria in the management of male infertility. Due to the several inherent limitations of semen analysis as a surrogate marker of male infertility, physicians should exercise caution when interpreting results. A template for semen analysis reports that incorporates the distribution of the semen characteristics of recent fathers in centiles rather than solely the minimum thresholds could aid clinicians to better understand how a given patient results compare with the reference population. Importantly, a male infertility evaluation must go far beyond a simple semen analysis, as it has to be complemented with a proper physical examination, a comprehensive history taking, and relevant endocrine, genetic, and other investigations.

Sandro C., Esteves.

2014-08-01

157

Association of soybean-based extenders with field fertility of stored ram (Ovis aries) semen: a randomized double-blind parallel group design.  

Science.gov (United States)

Two consecutive randomized double-blind field fertility experiments were conducted over a 4-month period and aimed at evaluating the association of two commercial soybean lecithin-based extenders (AndroMed [Minitub, Tiefenbach, Germany] and BioXcell [IMV Technologies, L'Aigle, France]) with pregnancy rates of chilled-stored (CS) and frozen-thawed (FT) ram semen. Semen samples with more than 2 × 10(9) sperm per mL and 70% progressive motile spermatozoa were collected via an artificial vagina from twelve proven fertile Chios rams, split-diluted with the above mentioned extenders, packaged in 0.25 mL straws and either stored at 5 ± 1 °C for 30 to 36 hours or frozen and thawed. Non-lactating multiparous ewes were inseminated in progestagen-synchronized estrus either with CS (AndroMed: N = 212 and BioXcell: N = 206; intracervical AI) or with FT (AndroMed: N = 114 and BioXcell: N = 92; laparoscopic intrauterine AI) semen. Ovulation was confirmed in all ewes based on determination of blood plasma progesterone (>1 ng/mL) 8 days post AI. Ewes were screened for pregnancy diagnosis by transabdominal ultrasonography 65 days post AI. BioXcell was superior to AndroMed in preserving the fertilizing potential of CS (P semen. In AndroMed-stored semen, young rams (1.5-2.5 years old, N = 8) had a pregnancy rate (59.1%; 124/210) lower than that (72.4%; 84/116) of mature rams (4.5 to 5.5 years, N = 4; P ram semen in BioXcell extender improved pregnancy rates of CS (66.7%; 88/132 vs. 83.9%; 94/112; P ram semen (P > 0.05). Ram-by-extender interactions were significant for pregnancy rates of CS and FT semen. Irrespective of extenders, overall pregnancy rates after intracervical and intrauterine AI were 75.1% and 62.2%, respectively (P ram semen. Selection of the ewes, farms, and extenders for intracervical AI programs can contribute to satisfactory fertility rates with semen preserved more than 24 hours at 5 °C. PMID:23219519

Khalifa, Tarek; Lymberopoulos, Aristotelis; Theodosiadou, Ekaterini

2013-02-01

158

Sperm Ubiquitination Correlation with Human Semen Quality  

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Full Text Available Background: Ubiquitin, an 8.5 kDa peptide that marks other proteins for proteasomal degradation, tags defective sperm during epididymal passage. Thus, sperm ubiquitination is a universal marker for sperm defects and can be used as a sperm function test. The objective of the present study was to examine the relationships between sperm ubiquitination and clinical semen parameters, using simplified immunofluorescence assays in order to establish ubiquitin as a biomarker of male infertility. Methods: Semen samples from 100 couples attending Avicenna Infertility Clinic, Tehran, Iran, were collected and analyzed according to WHO criteria. Each sample was washed and adjusted at 106 sperm/ml concentration. Sperm were coated on slides, using cytospin centrifugation and were fixed in buffered formaldehyde. Subsequently ubiquitinated spermatozoa were evaluated by direct immunofluorescence microscopy using FITC-labeled anti-ubiquitin antibodies. After counting at least 200 sperm per sample, while employing light microscopy, the percentage of ubiquitinated spermatozoa was recorded on the same fields through epifluorescence microscopy. Results: Negative correlations were obtained between sperm ubiquitination and sperm count (r=-0.278, P< 0.001, sperm concentration (r=-0.37, P< 0.001, viability (r=-0.407, P< 0.001, sperm morphology (r=-0.509, P< 0.001, rapid progressive motility (a (r=-0.246, P< 0.001 and slow progressive motility (b (r=-0.474, P< 0.001. There was a positive correlation between ubiquitinated sperm and the percentage of immotile spermatozoa (r=0.486, P< 0.000. Conclusion: Increased sperm ubiquitination is inversely associated with good semen quality parameters, supporting the use of ubiquitin as a biomarker for evaluation of human sperm quality.

MR Sadeghi

2008-07-01

159

Effect of exposing rams to a female stimulus before semen collection on ram libido and semen quality.  

Science.gov (United States)

Rams with strong libido and desirable semen characteristics can provide more insemination doses per ejaculate and produce more progeny, improving population genetic linkage to improve the accuracy of EBV. The objective of this study was to determine if teasing rams, either by sight and smell alone (Exp. 1), or physical contact (Exp. 2), could improve libido and semen quality of rams. In Exp. 1, there were 3 treatments in which rams were exposed to the sight and smell of the ewe for 1 h: control treatment (n = 5) in which rams were exposed to a ewe not in estrus; non-novel treatment (n = 6) in which rams were exposed to a ewe in estrus and the same ewe was used for semen collection; and novel treatment (n = 6) in which rams were exposed to a ewe in estrus and a different ewe in estrus was used for semen collection. In Exp. 2, rams were individually given full access to a ewe, which had a cotton apron fitted to cover her vulva, for 15 min. The 3 treatments in Exp. 2 were: control treatment (n = 5) in which rams were placed in a pen with a ewe not in estrus; a non-novel treatment (n = 5) in which rams were placed in a pen with a ewe in estrus and the same ewe was used for semen collection; novel treatment (n = 6) in which rams were placed in a pen with a ewe in estrus and a different ewe in estrus was used for semen collection. Experiment 1 was repeated for 5 consecutive days and Exp. 2 was repeated for 4 consecutive days. Data on reaction time, number of mounts, semen volume, semen concentration, sperm wave motion, and progressive linear motion (Exp. 1 only) were collected and analyzed as a randomized complete block design, where rams were initially blocked for breed and age. In Exp. 1, there was an effect of day (P semen volume, whereas there was also an effect of treatment (P semen concentration, which was most evident on d 1. In Exp. 2, there was an effect of treatment on reaction time (P semen volume (P = 0.08), which was most evident on d 1. This study demonstrates an acute effect on d 1 on semen concentration when rams were exposed to the sight and smell of a ewe in estrus. Alternatively, when rams were stimulated with physical contact of a ewe in estrus, an acute increase in semen volume was evident on d 1. These effects were not evident on subsequent days and thus the overall benefits on ram libido and semen quality of exposing rams to ewes in estrus are minimal. PMID:22665631

Fahey, A G; Duffy, P; Fair, S

2012-10-01

160

The effect of semen collection method and level of egg yolk on capability of dilution and storage of buck semen  

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Full Text Available The study was conducted to evaluate the effect of semen collection method for reduction of the deleterious interaction between the enzymes of bulbourethral gland and egg yolk during the dilution and storage of buck semen by three different level of egg yolk. Ten bucks were used in this study; the bucks were divided into two groups (five bucks in each group. Semen samples were collected once a week for four weeks from the bucks in first group using an artificial vagina, and from the animals in second group using an electroejaculator. The collected semen samples were diluted with sodium citrate extender with three different level of egg yolk (5, 10 and 20%. Extend semen samples were stored at 5 °C for three days. Computer assisted sperm analysis and Sperm Class Analyzer® were used for evaluation of the buck semen samples. Sperm motility parameters were evaluated which includes; percentage of motile sperm, percentage of progressive motile sperm, the value of the linear velocity (VSL, the value of the average velocity (VAP, the value of the curvilinear velocity (VCL, and the amplitude of lateral movement of the head (ALH. Results showed that all sperm motility parameters under the different level of egg yolk in semen samples that collected by artificial vagina were significantly higher than those which collected by electroejaculator. The percentage of motile sperm and progressive motile sperm of samples that collected by artificial vagina were higher at 10% of egg yolk, while these motility parameters were higher at 5% of egg yolk for semen samples that collected by electroejaculator. The differences between the two methods of semen collection in VCL and ALH were clear and the values were higher in samples that collected using the artificial vagina. The values of VSL, VAP and VCL of semen samples that collected by artificial vagina were higher at the second day than first day of semen storage under 10% of egg yolk. In conclusion, there are effects of the semen collection method on ability of dilution and storage of buck semen, and using of artificial vagina and 10% of egg yolk is recommended for buck semen dilution and storage.

N.N. Dhaher

2013-12-01

161

Semen A Altshuler: scientist, mentor, teacher  

Science.gov (United States)

International Conference `Resonances in Condensed Matter' is devoted to 100 years of the birthday of the Corresponding member of Academy of Sciences of the USSR, Professor of the Kazan University Semen Alexandrovich Altshuler (1911-1983). He is well known by pioneer works on EPR, the prediction and grounds for an existence of the neutron magnetic moment, the prediction and the theory of the acoustic paramagnetic resonance, and as a founder of the Kazan scientific school `Magnetic radiospectroscopy of condensed matter' (with E K Zavoiskii and B M Kozyrev)

Kochelaev, Boris I.

2011-10-01

162

Semen A Altshuler: scientist, mentor, teacher  

International Nuclear Information System (INIS)

International Conference 'Resonances in Condensed Matter' is devoted to 100 years of the birthday of the Corresponding member of Academy of Sciences of the USSR, Professor of the Kazan University Semen Alexandrovich Altshuler (1911–1983). He is well known by pioneer works on EPR, the prediction and grounds for an existence of the neutron magnetic moment, the prediction and the theory of the acoustic paramagnetic resonance, and as a founder of the Kazan scientific school 'Magnetic radiospectroscopy of condensed matter' (with E K Zavoiskii and B M Kozyrev)

163

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality  

Scientific Electronic Library Online (English)

Full Text Available It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO) pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress) and 3 months after [...] (non-stress) the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10(6)/mL), rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63%) and seminal plasma arginase activity (0.12 ± 0.01 vs 0.22 ± 0.01 U/mL) were significantly lower than in the non-stress situation, whereas seminal plasma NO (17.28 ± 0.56 vs 10.02 ± 0.49 µmol/L) was higher compared to the non-stress period (P

S., Eskiocak; A.S., Gozen; A., Taskiran; A.S., Kilic; M., Eskiocak; S., Gulen.

2006-05-01

164

Fertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methods  

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Full Text Available Abstract The aim of the project was to use current simple and practical laboratory tests and compare results with the foaling rates of mares inseminated with commercially produced frozen semen. In Exp. 1, semen was tested from 27 and in Exp. 2 from 23 stallions; 19 stallions participated in both experiments. The mean number of mares per stallion in both experiments was 37 (min. 7, max. 121. Sperm morphology was assessed and bacterial culture performed once per stallion. In Exp. 1, progressive motility after 0, 1, 2, 3, and 4 h of incubation using light microscopy, motility characteristics measured with an automatic sperm analyzer, plasma membrane integrity using carboxyfluorescein diacetate/propidium iodide (CFDA/PI staining and light microscopy, plasma membrane integrity using PI staining and a fluorometer, plasma membrane integrity using a resazurin reduction test, and sperm concentration were evaluated. In Exp. 2, the same tests as in Exp. 1 and a hypo-osmotic swelling test (HOST using both light microscopy and a fluorometer were performed immediately after thawing and after a 3-h incubation. Statistical analysis was done separately to all stallions and to those having ? 20 mares; in addition, stallions with foaling rates 20 mares, the artificial insemination dose showed a correlation coefficient of -0.58 (p

Koskinen E

2006-08-01

165

Use of factor scores for determining the relationship between body measurements and semen traits of cocks  

OpenAIRE

Semen evaluation is required to predict fertility. In most rural African communities, facilities for microscopic evaluation of semen are not available. Therefore, an indirect method of predicting semen traits of cocks is required by poultry farmers. The objective of this study was to use factor scores derived from factor analysis of body measurements to predict some semen traits of cocks. Correlation matrix was obtained by calculating the correlations between body measurements and semen trait...

Udeh Ifeanyichukwu

2012-01-01

166

Semen cryopreservation protocols of Mangalarga Marchador stallions  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions bet [...] ween the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.

Marcela Leite, Candeias; Marco Antonio, Alvarenga; Márcio Teoro do, Carmo; Heder Nunes, Ferreira; Mônica Russo Souto, Maior; Rodolpho de Almeida, Torres Filho; André Luís Rios, Rodrigues; Felipe Zandonadi, Brandão.

1989-19-01

167

Variations of protein profiles and calcium and phospholipase A2 concentrations in thawed bovine semen and their relation to acrosome reaction  

OpenAIRE

Just as calcium plays an integral role in acrosome capacitation and reaction, several spermatozoon proteins have been reported as binding to the ovum at fertilization. We examined the relationship between thawed bovine semen protein profiles, seminal plasma calcium ion concentration, spermatozoon phospholipase A2 (PLA2) activity and acrosome reaction. Electrophoretic profile analysis of spermatozoa and bovine seminal plasma proteins (total and membrane) revealed qualitative and quantitative d...

Alonso Marques, V.; Goulart, L. R.; Feliciano Silva, A. E. D.

2000-01-01

168

Evaluation of semen presumptive tests for use at crime scenes.  

Science.gov (United States)

The SERATEC PSA Semiquant and RSID-Semen tests are immunoassay kits that identify semen by detecting prostate-specific antigen (PSA) and semenogelin (Sg), respectively. Both kits were tested with semen, urine, blood, saliva, vaginal secretions and breast milk in order to determine their sensitivity and specificity. These results demonstrate that the SERATEC PSA kit is more sensitive than the RSID-Semen kit with a limit of detection of 200 ng/mL as opposed to 8.0 x 10(3) ng/mL. The RSID-Semen kit gave no false-positives or -negatives compared with 2.9% false-negatives with the SERATEC PSA kit. Results from postcoital samples show the RSID-Semen kit to be more effective, indicating that this kit is more suitable for semen identification in the Haven Suites. As a more robust and cost-effective kit, the SERATEC PSA test is recommended for use at crime scenes. The ability to obtain DNA profiles from the buffer of both kits demonstrates the potential benefit of these kits in a rape investigation. The use of these kits at crime scenes would provide an invaluable contribution by prioritizing samples for subsequent analysis, thereby allowing greater efficiency with investigation times. PMID:21595415

Laffan, Aine; Sawyer, Ian; Quinones, Ignacio; Daniel, Barbara

2011-01-01

169

EFFECTS OF PHOTOSTIMULATION ON SEMEN PRODUCTION IN RHODE ISLAND ROOSTERS  

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Full Text Available In mammals the length of daylight has an oscillatory influence on semen production. It is known that in mammalian males highest semen output occurs mainly in spring and fall. It is possible that there is the same pattern in rooster semen production despite the anatomic differences regarding the testis location and, obviously local temperature. Considering these facts the present trial was set up in order to reveal effects of prolonged daylight – photo stimulation – on semen production in young roosters. All young roosters in the trial were divided in 3 groups, according to the age when photo stimulating schedule started. Photo stimulation was performed by moving young roosters from an 8h/day light to 14h / day light. Attempts of collecting semen up to the age of 20 weeks have failed showing relationship between body general development and semen output. Under prolonged light semen parameters as volume, motility and concentration changed from one week to the other. However, light is not the single factor inducing sexual maturity of the genital tract, but it could be used in young roosters in order to stimulate feed intake and thus overall body growth and development.

DANIELA LADOSI

2013-12-01

170

Persistent organic pollutants and semen quality: The LIFE Study.  

Science.gov (United States)

Growing evidence suggests that persistent environmental chemicals such as polychlorinated biphenyls may adversely affect human fecundity. The purpose of this study was to evaluate associations between persistent environmental chemicals and semen quality among 501 male partners of couples discontinuing contraception for purposes of becoming pregnant. Men provided a blood specimen and two fresh semen samples collected approximately a month apart that underwent next day analysis for 35 semen quality endpoints. Serum samples were analyzed for 36 polychlorinated biphenyls (congeners #18, 28, 44, 49, 52, 66, 74, 87, 99, 101, 114, 118, 128, 138, 146, 149, 151, 153, 156, 157, 167, 170, 172, 177, 178, 180, 183, 187, 189, 194, 195, 196, 201, 206, 209); 1 polybrominated biphenyl (#153); 9 organochlorine pesticides; and 10 polybrominated diphenyl ethers (congeners #17, 28, 47, 66, 85, 99, 100, 153, 154183) using high resolution mass spectrometry. To estimate the effect of chemicals on semen quality, we regressed each semen marker on each chemical while adjusting for research site, age, body mass index, serum lipids, and cotinine levels. Males with chemical concentrations in the fourth quartile, as compared to the first quartile, showed significant associations for several individual chemicals in each chemical class and type of semen quality parameter indicating negative and positive associations with semen quality. Polybrominated diphenyl ethers in particular were associated with several measures of increased abnormal morphology. These exploratory results highlight the role of environmental influences on male fecundity, and are of particular interest given the ubiquitous exposures to these compounds. PMID:25441930

Mumford, Sunni L; Kim, Sungduk; Chen, Zhen; Gore-Langton, Robert E; Boyd Barr, Dana; Buck Louis, Germaine M

2014-11-28

171

Factors predicting early improvement in semen parameters following varicocele surgery  

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Full Text Available Background: Varicocelectomy does not improve semen parameters and pregnancy rates in all cases. Various studies have been done to find out factors which predict better outcomes of varicocelectomy so that only such patients may be selected to undergo the surgery. The present study is an attempt to identify such factors by prospective cohort method. Methods: A prospective cohort study was conducted on 25 patients undergoing varicocelectomy for infertility at St John medical college hospital, Bangalore from 01-06-2012 to 31-05-2013. Clinical data, semen analysis, scrotal imaging and hormonal assays were done and postoperatively semen analysis was done after three months. The data was analysed to find out predicting factors for improvement of semen parameters. Results: Twenty five patients underwent varicocele surgery, all of them showed improvement of semen parameters. Fifteen of them had more than 50% of improvement. Serum FSH and testosterone levels were found to be predictive of semen parameter improvement. Conclusions: Preoperative low serum FSH and high testosterone concentration were factors predicting early improvement in semen parameters following varicocele surgery in infertile males. [Int J Reprod Contracept Obstet Gynecol 2014; 3(4.000: 1027-1032

Shreeharsha Mallappa Awati

2014-08-01

172

Effect of Seasonal Variation on Semen Quality and Herd Fertility  

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Full Text Available The data of the present study were collected from Bangladesh Milk Producer`s Co-operative Union Limited (BMPCUL at Baghabarighat, Sirajgonj to investigate the bull performance among various seasons through semen quality and 30-day non return rate. To evaluate the seasonal variation on semen quality and bull fertility the experimental period was divided into three seasons; a Summer season (March to June, b Rainy season (July to October and c Winter season (November to February. Data on 245 ejaculates and 12,750 services of almost same aged 5 Sahiwal bulls (B1, B2, B3, B4 and B5 over a period of 5 years were collected from Animal Breeding section and Cattle Feed Unit of BMPCUL. The performance of five bulls was compared in three seasons i.e. summer, rainy and winter and summer was observed to be the best for most parameters studied. Seasons had significant (p<0.05 effect on semen volume, initial and post- thawing sperm motility and pH of semen but not on sperm motility of diluted semen and fertility. The significant (p<0.01 bull x season interaction was noticed in semen volume, sperm motility at initial and post-thawing stages, pH and fertility but not on sperm motility of diluted semen.

M.P. Mostari

2005-01-01

173

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen  

OpenAIRE

The use of several types of gelling extenders for the storage of semen from several domestic species in the solid state has been shown to have beneficial effects on some semen quality parameters. The objective of this study was to evaluate the effect of a new high-viscosity semen extender, Zoosperm ND-5 3D® (Import-Vet, Centelles, Spain), on the the quality of boar spermatozoa at preserved at 17ºC for 7 days. Sodium alginate was used for the first time to increase the viscosity of the exten...

Maria Cruz Gil; Francisco Javier Barón; Jesús Manuel Guerrero; Luis Jesús García-Marín; Javier Gil

2014-01-01

174

Association of Vitamin E with Rapid Thawing on Goat Semen  

OpenAIRE

The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100??M. Semen was packaged into 0.25?mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (?155°C) duri...

Jurandy Mauro Penitente-Filho; Fabrício Albani Oliveira; Carolina Rodriguez Jimenez; Erly Carrascal; Xe Sar Oliveira Dias, J. Xfa Lio C.; Gisele Dias Oliveira; Renata Gomes Silveira; Camila Oliveira Silveira; Ciro Alexandre Alves Torres

2014-01-01

175

Should single layer centrifugation of dog semen be done before or after the semen is cooled?  

Science.gov (United States)

The aim of this study was to assess the effectiveness of sperm selection by single layer centrifugation (SLC) on canine sperm quality when SLC was performed before or after the cooling process, or when double SLC (before and after cooling) was performed. Twenty ejaculates from four dogs were divided into four aliquots as follows: unselected: no SLC was performed; SLC prior to cooling (SLC-PC): sperm selection was carried out before cooling; SLC after cooling (SLC-AC): sperm selection was performed after cooling; and double SLC: sperm selection was carried out before and after cooling. Sperm motility (by computer-assisted semen analysis), morphology (Diff-Quick staining), sperm membrane integrity (Vital-Test kit) and acrosome integrity (double fluorescent stain) were assessed in re-warmed semen samples. Four sperm subpopulations (sP) were detected using a pattern analysis technique (sP1: highly active, non-progressive; sP2: low velocity, highly progressive; sP3: less vigorous, poorly progressive; sP4: highly progressive motility). A higher proportion of sperm were classified as sP4 in SLC-AC samples. Most of the sperm parameters assessed showed higher values in the SLC-AC group. We conclude that SLC-AC is the best protocol to improve sperm quality in chilled canine semen in comparison to the other procedures tested. PMID:25653394

Gálvez, M J; Ortiz, I; Hidalgo, M; Morrell, J M; Dorado, J

2015-04-01

176

Bulls grazing Kentucky 31 tall fescue exhibit impaired growth, semen quality, and decreased semen freezing potential.  

Science.gov (United States)

Serum prolactin (PRL) and testosterone concentrations, body weight, body composition, semen quality, and semen freezing potential for bulls grazing the toxic tall fescue (Lolium arundinaceum [Schreb.] Darbysh. = Schedonorous arundinaceum [Schreb.] Dumort.) cultivar Kentucky 31 (E+) compared with a novel endophyte cultivar lacking ergot alkaloids (E-) were evaluated. Angus bulls were allotted to treatment (Day 0) and grazed E+ or E- for 155 days. Treatment-by-day interaction was significant (P concentrations with E+treated bulls exhibiting reduced PRL values compared with E- control bulls, but no differences were observed for serum testosterone concentrations (P > 0.05). Further, bulls on the E+ treatment exhibited decreased total gain, average daily gain, and body weight by Day 140 (P concentration with lower values observed for bulls on the E+ treatment (P < 0.05). The percent normal morphology was reduced in ejaculates from E+ bulls compared with E- bulls (P < 0.05), and the difference was due to an increase in abnormal sperm present in the E+ ejaculates from Day 84 to 140 (P < 0.05). In addition, spermatozoa motility and progressive motility were decreased on thawing in semen samples from E+ bulls compared with E- bulls (P < 0.05). PMID:25459421

Pratt, S L; Stowe, H M; Whitlock, B K; Strickland, L; Miller, M; Calcatera, S M; Dimmick, M D; Aiken, G E; Schrick, F N; Long, N M; Duckett, S K; Andrae, J G

2015-02-01

177

Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen.  

Science.gov (United States)

Semen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to compare the influence of extender (TEST versus INRA96®) and penetrating cryoprotectants (3% glycerol, 5% glycerol and 4% methylformamide) on post-thaw semen quality. We demonstrate that not only the freezing process, but also the quality of the semen before freezing, significantly influences the freezability of Asian elephant semen. Pre-freeze motility, viability, semen volume, semen pH, sperm concentration and the incidence of sperm mid-piece and tail abnormalities all significantly (p<0.05) affected post-thaw semen quality. While extender and cryoprotectant did not significantly affect any of the above semen quality parameters post-thaw, the skim-milk based extender (INRA96®) preserved DNA integrity better (p<0.05) than the egg yolk extender (TEST). Considerable between-ejaculate variation in all post-thaw semen quality parameters was also noted. It is concluded that strict criteria for semen quality is essential for the selection of Asian elephant bull ejaculates suitable for cryopreservation; stricter initial selection should improve the mean post-thaw quality. PMID:23168056

Imrat, P; Suthanmapinanth, P; Saikhun, K; Mahasawangkul, S; Sostaric, E; Sombutputorn, P; Jansittiwate, S; Thongtip, N; Pinyopummin, A; Colenbrander, B; Holt, W V; Stout, T A E

2013-02-01

178

Relationships between seminal plasma arginase activity and spermatological parameters in rams.  

Science.gov (United States)

This study was conducted to evaluate the correlation between seminal plasma arginase activity and spermatological parameters in rams. In this study, five fertility-proven Awassi rams were used as material. Six ejaculates were collected from each ram by an artificial vagina. Spermatological parameters (semen volume, mass activity, sperm motility and concentration and abnormal sperm rate) were immediately determined in each ejaculate. For enzyme assay, the semen samples were centrifuged and stored at -20 °C for the analysis of arginase activity. The average seminal plasma arginase activity was 0.61 ± 0.20 U (mg protein)(-1) . There was a positive correlation between arginase activity and semen volume (r = 0.412, P semen mass activity (r = 0.610, P semen analysis parameters in rams. PMID:21848887

Gür, S; Kandemir, F M

2012-04-01

179

Fatores de risco na transmissão do lentivírus caprino pelo sêmen / Risk factors in caprine lentivirus transmission through semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O objetivo deste trabalho foi avaliar a presença do DNA pró-viral do lentivírus caprino (LVC) em ejaculados de machos infectados naturalmente, e verificar a influência da lavagem do sêmen e da presença de inflamação testicular sobre a carga viral. Foram realizadas oito coletas de sêmen de sete repro [...] dutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, o ejaculado era lavado, para retirada do plasma seminal, e na outra, não. O DNA pró-viral do LVC foi identificado pela reação em cadeia da polimerase Nested (PCR Nested), e pelo isolamento viral. O vírus foi isolado em 7,1% das amostras. A PCR identificou o DNA pró-viral em 35,7% do total das amostras: 17,9% nas amostras lavadas e 53,6% das amostras de sêmen integrais. O dano ao testículo permite maior fluxo do vírus para o sêmen, pois antes do dano, 21,4% das amostras foram positivas e pós-dano, 50%. A transmissão do LVC pelo sêmen de reprodutores caprinos é potencializada pela presença de inflamações testiculares e pelo fato de o sêmen criopreservado conter o LVC na forma infectante. Abstract in english The objective of this work was to evaluate the presence of the DNA provirus of the caprine lentivirus (LVC) in ejaculates of naturally infected males, and to verify the influence of the wash of the semen as well as the presence of testicle inflammation on the viral load. Eight semen collections of s [...] even soropositive reproducers were accomplished, four before testicle injury and four after injury. Amongst the collections carried out at the same week, in one the ejaculate was washed, to withdraw the plasma seminal, and in the other it was not. The provirus DNA was identified both by Nested polymerase chain reaction technique (Nested PCR) and by the viral isolation. The virus was isolated in 7.1% of the samples. The PCR identified the provirus DNA in 35.7% of all samples, 17.9% in the washed samples and 53.6% of the integral semen samples. The injury of the testicle tends to greater flow of virus for the semen, therefore, before injury, 21.4% of the samples were positive and after-injury, 50%. Risk of transmission of the LVC by semen of goat reproducers is strengthened by the presence of testicle inflammations and the fact that the criopreserved semen contains the LVC in infecting form.

Alice, Andrioli; Aurora Maria Guimarães, Gouveia; Almir de Sousa, Martins; Raymundo Rizaldo, Pinheiro; Diones Oliveira, Santos.

1313-13-01

180

Risk factors for bacterial contamination during boar semen collection.  

Science.gov (United States)

The aim of this study was to evaluate the influence of multiple factors on bacterial contamination in 213 ejaculates from four boar studs. Semen contamination by aerobic mesophiles increased in ejaculates where the preputial fluid flowed into the collection container, collection glove was dirty, preputial hair was long (>1.0 cm), the collection lasted >7 min and boars were older than 18 months. An increase in coliforms occurred when preputial fluid dripped into the collection container, collections lasted >7 min or when penis escaped during collection. Semen contamination increased when two or more factors related to hygiene (poor hygiene of the boar, dirty preputial ostium, large preputial diverticulum, long preputial hair, dirty gloves, preputial liquid trickling from the hand of the technician into the semen container and penis escaping) were present. A vigilant protocol of collection must be followed to minimize bacterial contamination, especially avoiding dripping of preputial liquid into the semen container. PMID:23891384

Goldberg, Ana Maria G; Argenti, Laura E; Faccin, Jamil E; Linck, Lídia; Santi, Mônica; Bernardi, Mari Lourdes; Cardoso, Marisa R I; Wentz, Ivo; Bortolozzo, Fernando P

2013-10-01

181

Relación entre calidad del semen y la edad / Relationship between quality of semen and age  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Objetivo: Determinar la relación entre la calidad del semen humano y la edad. Material y Métodos: El espermatograma se realizó siguiendo el Manual de Laboratorio de la OMS para el examen del Semen Humano y de la Interacción Moco Cervical y Semen (1999), de los exámenes realizados entre julio 2003 a [...] diciembre 2008. Se estudiaron 2 441 casos de varones que cumplen con los criterios de inclusión. Resultados: La motilidad A+B fue de 51,55% para varones de 20 a 29 años; los espermatozoides normales fue de 77,73% para varones mayores de 50 años; el recuento espermático (mill/ml) fue de 61,09 para varones mayores de 50 años.La evaluación de la motilidad espermática tuvo como coeficiente de correlación lineal múltiple de 0,222 y coeficiente de determinación de 0,049; en la morfología espermática, coeficiente de correlación lineal de 0,0622 y coeficiente de determinación de 0,0039; en el recuento espermático, coeficiente de correlación lineal múltiple de 0,465 y coeficiente de determinación de 0,216. Conclusiones: existe una tendencia inversa entre la motilidad y la edad, una tendencia directa entre el recuento espermático y la edad, y una tendencia constante entre morfología espermática y edad. Abstract in english Objectives: To determine the relationship between the quality of human semen and age. Methods: A spermatogram was performed following the WHO´s laboratory manual to evaluate human sperm and the interaction between cervical mucus and semen (1999) from July 2003 and December 2008. We studied 2441 male [...] cases that fulfilled the inclusion criteria. Results: A+B motility was 51.55% for 20-29 years of age male participants; normal spermatozoids were found in 77.73% of males above 50 years of age; the spermatic count (mill/ml) was 61.09 for males above 50 years of age. Spermatic motility had a multiple lineal correlation coefficient of 0.222 and a determination coefficient of 0.049; respective values for the spermatic count were 0.465 and 0.216. Conclusions: There is an inverse trend between motility and age, a direct trend between spermatic count and age, and a constant trend between spermatic morphology and age.

John, Chávez; José, Yarlequé; Elmer, Avalos; Ruth, Barrientos-Marka; MarcoAntonio, García.

2012-07-01

182

9 CFR 98.38 - Restrictions on the importation of swine semen from the APHIS-defined EU CSF region.  

Science.gov (United States)

... false Restrictions on the importation of swine semen from the APHIS-defined EU CSF region. 98.38... IMPORTATION OF CERTAIN ANIMAL EMBRYOS AND ANIMAL SEMEN Certain Animal Semen § 98.38 Restrictions on the importation of...

2010-01-01

183

9 CFR 85.10 - Interstate movement of swine semen and swine embryos for insemination of or implantation into swine.  

Science.gov (United States)

...false Interstate movement of swine semen and swine embryos for insemination...10 Interstate movement of swine semen and swine embryos for insemination of or implantation into swine. Swine semen and swine embryos moved...

2010-01-01

184

Artificial insemination and cryopreservation of semen from nondomestic birds  

Science.gov (United States)

Studies of Al and cryopreservation of semen from nondomestic birds began because of the increased emphasis on conservation of avian species threatened with extinction. Over the years, aviculturists have developed techniques for Al and cryopreservation of semen obtained from a variety of birds ranging from passerines to Andean condors. Generally, for each new species, we develop a practical semen collection technique and then evaluate the semen. A commercial semen extender (Beltsville Poultry Semen Extender) is modified and used to dilute the semen and provide support for the sperm during the freezing process (the pH and osmolality of the extender is adjusted to reflect the pH and osmolality of the semen being frozen). We find that the freezing schedule developed by Sexton (1977), which utilizes dimethylsulfoxide (DMS0) as cryoprotectant, works well for many species. We cool the sample sequentially in an ethanol bath, in liquid nitrogen vapor, and lastly in liquid nitrogen. Although we have experimented with a variety of freezing protocols, we prefer a 15-min equilibration period in DMSO at 5 C. We begin the freezing process by cooling at -1 C/min from 5 to -20 C in the ethanol bath. The samples are transferred into a vapor tank at a location just above liquid nitrogen and frozen at -50 C/min to -80 C. To complete the freezing process, the samples are plunged into the liquid nitrogen in the bottom of the vapor tank. The samples remain in liquid nitrogen until they are thawed just before insemination. If necessary, the freezing equipment can be transported in a van to remote locations.

Gee, G.F.

1995-01-01

185

Bull Semen Collection and Analysis for Artificial Insemination  

OpenAIRE

Insemination is acknowledged as a breeding method that contributes to improvement of farm animal populations, particularly of cattle. Artificial insemination allows for maximum use of the most valuable breeders and, at the same time, for significant increase of breeding advance. Moreover, using semen of proved quality reduces the spread of sexually transmitted diseases. The purpose of this study was to present the process of collection and analysis of bulls’ semen in the Mazovian Centre of ...

Karolina Barszcz; Dariusz Wiesetek; Michal Wasowicz; Marta Kupczynska

2012-01-01

186

Effect of Seasonal Variation on Semen Quality and Herd Fertility  

OpenAIRE

The data of the present study were collected from Bangladesh Milk Producer`s Co-operative Union Limited (BMPCUL) at Baghabarighat, Sirajgonj to investigate the bull performance among various seasons through semen quality and 30-day non return rate. To evaluate the seasonal variation on semen quality and bull fertility the experimental period was divided into three seasons; a) Summer season (March to June), b) Rainy season (July to October) and c) Winter season (November to February). Data on ...

Mostari, M. P.; Hasanat, M. S.; Azmal, S. A.; Monira, K. N.; Khatun, H.

2005-01-01

187

Semen analysis and sperm function tests: How much to test?  

OpenAIRE

Semen analysis as an integral part of infertility investigations is taken as a surrogate measure for male fecundity in clinical andrology, male fertility, and pregnancy risk assessments. Clearly, laboratory seminology is still very much in its infancy. In as much as the creation of a conventional semen profile will always represent the foundations of male fertility evaluation, the 5th edition of the World Health Organization (WHO) manual is a definitive statement on how such assessments shoul...

Vasan, S. S.

2011-01-01

188

Semen quality analysis and the idea of normal fertility  

OpenAIRE

The World Health Organization (WHO) has extensively revised its manual for semen analysis, and seeks to provide reference limits for semen quality parameters. This raises the question of what is meant by 'normal' (excluding the use of this term to denote a Gaussian distribution). It could be taken in a purely statistical sense, using a biologically arbitrary cut-off point to denote an abnormal level, typically the extreme 5 percent of the population. Alternatively, 'normal' could be defined a...

Joffe, Michael

2010-01-01

189

Semen decontamination for the elimination of seminal HIV-1.  

Science.gov (United States)

The risk of human immunodeficiency virus (HIV) transmission to the female partner, or potential offspring of an HIV-1 infected man can be reduced using semen decontamination procedures before assisted reproductive treatment (ART). The objective of this study was to determine the efficiency of decontaminating semen samples (n?=?186) from 95 HIV-1 sero-positive patients. Aliquots of neat semen were submitted for viral validation by qualitative and quantitative polymerase chain reaction. Semen samples were processed by density gradient centrifugation in combination with a ProInsert™ tube after which aliquots of the processed sperm samples were analysed for the presence of HIV-1. Fifty-four percent of all tested neat semen samples tested positive for HIV-1 DNA, RNA or both (13.4%, 11.3% and 29.0%, respectively). From a total of 103 processed sperm samples that were submitted for viral validation, two samples tested positive for HIV-1 DNA and none for RNA. In conclusion, semen processing with the ProInsert™ followed by viral validation of processed sperm samples should be carried out when providing ART to couples where the male partner is HIV-1 sero-positive. PMID:25596908

Fourie, Jozef Markus; Loskutoff, Naida; Huyser, Carin

2015-03-01

190

Seasonal Variations in Semen Characteristics in Arabic Rams  

Directory of Open Access Journals (Sweden)

Full Text Available The aim of this study was to evaluate the seasonal variations in semen characteristics in Arabic rams. 8 adult Arabic rams at the age of 2-3 years were used for this research. Semen was collected with electro ejaculator every 15 days for a period of 6 months (3 months of in breeding season and 3 months of non breeding season. Semen samples of these rams were subjected to the parameters including semen volume, sperm motility, sperm concentration, abnormal and live sperm percentage. Semen quality difference between breeding and non breeding seasons was significant. Semen volume, sperm motility, percent of live sperm, the percentage of abnormal sperm and sperm concentration determined in breeding and non breeding seasons had significant differences. Therefore, better progressive motility of sperm, sperm concentration, percent of live sperm and low percentage of abnormal sperm clearly explained high fertility potential of male in breeding season when compared with non breeding season. In conclusion, this study indicated that sexual performance of Arabic rams completely dependent on season.

A. Hamidi, M. Mamoei, Kh. Mirzadeh, S. Tabatabaei* and H. Roshanfekr

2012-01-01

191

Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination  

OpenAIRE

Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica) queen spermatheca werestudied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05) between the 12th and 24th month.The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminatedtwice with a Hyes solution/semen...

Andrea Galli; Donatella Balduzzi; Marco Lodesani

2010-01-01

192

The clinical significance of corrected seminal plasma prolactin level in men with asthenospermia  

International Nuclear Information System (INIS)

Objective: To evaluate the clinical significance of corrected seminal prolactin assay in men with asthenospermia. Methods: Routine semen analysis and seminal plasma prolactin assay were performed on the men with asthenospermia, oligo-asthenospermia, normospermia. Prolactin was assessed by radioimmunoassay. The relationship between the level of corrected seminal plasma prolactin and the quality of semen was analyzed. Results: The mean level of the corrected seminal prolactin in the men with asthenospermia was (26.1±12.8) ?g/L and was significantly higher than that of the men with normospermia. Seminal plasma prolactin concentration showed linear increasing alongside with the decreasing of the semen motility and motility degrees. Conclusion: The detection of corrected seminal plasma prolactin level will provide an objective index for evaluating the semen quality of asthenospermic men

193

Human semen assays for workplace monitoring. [Monitoring of hazardous materials by determining effects on semen of personnel  

Energy Technology Data Exchange (ETDEWEB)

Decades of human semen studies have yielded compelling evidence that sperm can be used to access reproductive potential and diagnose pathology. With these studies as background, the small number of detailed semen studies of men exposed to physical and chemical agents point with optimism to the application of human semen assays as efficient, effective means to monitor for reproductive hazards in the workplace. Sperm are the most accessible of human gonadal tissue and provide a means of monitoring exposure induced changes in the human testes, changes which may result in infertility and increased frequencies of genetically abnormal gametes. The focus on semen has precipitated the development of new sperm bioassays which use older conventional andrological methods (i.e., sperm counts, motility, and morphology) as well as recently developed high speed flow and scanning methods for automated cytological analyses. The status of these sperm assays for workplace surveillance is reviewed, procedures are suggested with examples of use, and their effectiveness is evaluated. The available mouse models of induced semen changes are briefly described and the importance of these models for evaluating the genetic implications of findings in human semen is discussed.

Wyrobek, A.J.; Gledhill, B.L.

1978-11-07

194

The semen microbiome and its relationship with local immunology and viral load in HIV infection.  

Science.gov (United States)

Semen is a major vector for HIV transmission, but the semen HIV RNA viral load (VL) only correlates moderately with the blood VL. Viral shedding can be enhanced by genital infections and associated inflammation, but it can also occur in the absence of classical pathogens. Thus, we hypothesized that a dysregulated semen microbiome correlates with local HIV shedding. We analyzed semen samples from 49 men who have sex with men (MSM), including 22 HIV-uninfected and 27 HIV-infected men, at baseline and after starting antiretroviral therapy (ART) using 16S rRNA gene-based pyrosequencing and quantitative PCR. We studied the relationship of semen bacteria with HIV infection, semen cytokine levels, and semen VL by linear regression, non-metric multidimensional scaling, and goodness-of-fit test. Streptococcus, Corynebacterium, and Staphylococcus were common semen bacteria, irrespective of HIV status. While Ureaplasma was the more abundant Mollicutes in HIV-uninfected men, Mycoplasma dominated after HIV infection. HIV infection was associated with decreased semen microbiome diversity and richness, which were restored after six months of ART. In HIV-infected men, semen bacterial load correlated with seven pro-inflammatory semen cytokines, including IL-6 (p = 0.024), TNF-? (p = 0.009), and IL-1b (p = 0.002). IL-1b in particular was associated with semen VL (r(2)? = 0.18, p = 0.02). Semen bacterial load was also directly linked to the semen HIV VL (r(2) = 0.15, p = 0.02). HIV infection reshapes the relationship between semen bacteria and pro-inflammatory cytokines, and both are linked to semen VL, which supports a role of the semen microbiome in HIV sexual transmission. PMID:25058515

Liu, Cindy M; Osborne, Brendan J W; Hungate, Bruce A; Shahabi, Kamnoosh; Huibner, Sanja; Lester, Richard; Dwan, Michael G; Kovacs, Colin; Contente-Cuomo, Tania L; Benko, Erika; Aziz, Maliha; Price, Lance B; Kaul, Rupert

2014-07-01

195

SEMEN QUALITY ASSESSMENT OF NEW ZEALAND WHITE RABBIT BUCKS  

Directory of Open Access Journals (Sweden)

Full Text Available Rabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37°C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detectedRabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37°C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detected.

Martyna B?aszczyk

2013-02-01

196

Seasonal Variations in Certain Physical and Biochemical Attributes of Semen from Cholistani Bulls  

Directory of Open Access Journals (Sweden)

Full Text Available In the present study, effects of stress-free and stressful seasons on certain physical and biochemical attributes of semen of Cholistani AI bulls were investigated. A total of 382 ejaculates from six bulls were collected at weekly intervals during four seasons viz. stress-free autumn (October-November, stressful winter (December-January, stressful dry summer (May-June and stressful wet summer (July-August. These ejaculates were evaluated for various physical and biochemical attributes. Overall mean values (±SEM for ejaculatory volume, number of ejaculates per bull, mass motility, individual sperm motility, sperm concentration, number of doses of semen frozen per bull, percentages of live, morphologically normal sperm and those with intact acrosome were 4.92±0.14 mL, 1.89±0.02, 2.26±0.06, 63.51±1.03%, 989.73±27.4 million/mL, 208.26±8.42, 86.74±0.55%, 86.34±0.51% and 86.64±0.48%, respectively. In general, all the parameters revealed significantly (P<0.05 better results in stressful seasons as compared to stress-free season. Overall mean values for Na+, K+, cholesterol and glucose in the seminal plasma were 102.79±3.95 & 36.65±0.99 mEq/L, 152.02±3.17 & 127.22±3.65 mg/dL, respectively. Amongst these, Na+ was higher (P<0.05 during dry summer, whereas K+ was higher in dry summer and winter. Cholesterol and glucose were elevated (P<0.05 during dry summer season. Correlation analysis revealed significant positive correlations between mass and individual sperm motility, morphologically normal and live sperm and sperm acrosome integrity and live sperm (P<0.01. Among biochemical attributes of seminal plasma, Na+ and K+, cholesterol and K+ and glucose and Na+ were correlated (P<0.01. Considering seminal plasma biochemical constituents and physical attributes, only seminal plasma cholesterol was positively correlated with ejaculatory volume (P<0.01. In conclusion, various physical and biochemical attributes of Cholistani bull semen were better in stressful seasons as compared to stress free season.

Umer Farooq, Ahmad Ijaz1, Nazir Ahmad2*, Habib Rehman1 and Hafsa Zaneb3

2013-11-01

197

plasmas  

Science.gov (United States)

As-deposited HfO2 films were modified by CHF3, C4F8, and mixed C4F8/O2 plasmas in a dual-frequency capacitively coupled plasma chamber driven by radio frequency generators of 60 MHz as the high frequency (HF) source and 2 MHz as the low frequency source (60/2 MHz). The influences of various surface plasma treatments under CHF3, C4F8, and C4F8/O2 were investigated in order to understand the chemical and structural changes in thin-film systems, as well as their influence on the electrical properties. Fluorine atoms were incorporated into the HfO2 films by either CHF3 or C4F8 plasma treatment; meanwhile, the C/F films were formed on the surface of the HfO2 films. The formation of C/F layers decreased the k value of the gate stacks because of its low dielectric constant. However, the addition of O2 gas in the discharge gases suppressed the formation of C/F layers. After thermal annealing, tetragonal HfO2 phase was investigated in both samples treated with CHF3 and C4F8 plasmas. However, the samples treated with O-rich plasmas showed monoclinic phase, which indicated that the addition of O plasmas could influence the Hf/O ratio of the HfO2 films. The mechanism of the t-HfO2 formation was attributed to oxygen insufficiency generated by the incorporation of F atoms. The capacitors treated with C4F8/O2 plasmas displayed the highest k value, which ascribed that the C/F layers were suppressed and the tetragonal phase of HfO2 was formed. Good electrical properties, especially on the hysteresis voltage and frequency dispersion, were obtained because the bulk traps were passivated by the incorporation of F atoms. However, the H-related traps were generated during the CHF3 plasma treatments, which caused the performance degradation. All the treated samples showed lower leakage current density than the as-deposited HfO2 films at negative bias due to the reduced trap-assisted tunneling by the incorporation of F to block the electrons transferring from metal electrode to the trap level.

Zhang, H. Y.; Jin, C. G.; Yang, Y.; Ye, C.; Zhuge, L. J.; Wu, X. M.

2014-12-01

198

The effects of alpha-chlorohydrin on the composition of rat and rabbit epididymal plasma: a possible explanation of species difference.  

Science.gov (United States)

The relationship between the antifertility effect of alpha-chlorohydrin and changes in composition of luminal plasma from the cauda epididymidis of rats and rabbits has been investigated. At each dose regimen studied, the fertilizing capacity of rats treated with alpha-chlorohydrin was reduced to zero. The levels of sodium, potassium, glycerylphosphorylcholine (GPC), acid phosphatase and alkaline phosphatase in epididymal plasma were not markedly affected by drug treatment. The most noticeable change was a considerable increase in the concentration of lactic dehydrogenase (LDH) at all dose levels and of glutamic-oxaloacetic transaminase (GOT) after 7 days of treatment with 8 and 16 mg/kg. The effect of cold shock on the composition of epididymal plasma showed that LDH and GOT are, at least in part, derived from spermatozoa. In contrast, alpha-chlorohydrin did not have an antifertility action in the rabbit, and the only notable change in the compositon of epididymal plasma was an increase in the level of GPC. These results provide evidence that, in the rat, alpha-chlorohydrin or a metabolite primarily exerts its antifertility effect by a direct action on the spermatozoa, whilst in the rabbit a barrier may exist to the entrance of the drug into the lumen of the epididymal duct. PMID:1195243

Back, D J; Glover, T D; Shenton, J C; Boyd, G P

1975-10-01

199

Toxicity of cryoprotectants to honey bee semen and queens.  

Science.gov (United States)

Given the threats to the intraspecific biodiversity of Apis mellifera and the pressure on bee breeding to come up with disease-tolerant lines, techniques to cryopreserve drone semen are of great interest. Freeze-thawed drone semen of high viability and/or motility has repeatedly been obtained, but fertility of such semen, when it was measured, was always low. The cryoprotective agent (CPA) most frequently used with drone semen is dimethyl sulfoxide (DMSO), although this substance has been suspected of causing genetic damage in sperm. No form of sperm washing is currently performed. Using a membrane permeability assay, we measured the short-term toxicity of four possible replacements for DMSO, 1,3-propane diol, 2,3-butane diol, ethylene glycol, and dimethyl formamide. We also tested whether the practice of inseminating queens with CPA-containing semen affects sperm numbers in the storage organs of queens, or sperm fertility. Finally, we tested whether CPA-toxicity in vivo can be reduced by using mixtures of two CPAs, DMSO, and ethylene glycol. Our results show that, although short-term toxicity of all CPAs tested was low, the presence of single CPAs in insemination mixtures at concentrations required for slow freezing greatly reduced the number of sperm reaching the spermatheca. Contrary to earlier reports, this was also true for DMSO. Ethylene glycol was additionally shown to reduce the viability of spermatozoa reaching the storage organ. Mixtures of DMSO and EthGly performed better than either substance used singly at the same concentration. We conclude that the toxicity of CPAs, including DMSO, on honey bee semen and/or queens has been underestimated in the past. This could partly explain the discrepancy between in vitro and in vivo quality of cryopreserved drone semen, described by others. Combinations of several CPAs and techniques to partly remove CPAs after thawing could help to solve this problem. PMID:22115807

Wegener, J; Bienefeld, K

2012-02-01

200

Detection of Neospora caninum DNA in semen of experimental infected rams with no evidence of horizontal transmission in ewes.  

Science.gov (United States)

Recent reports from New Zealand indicate Neospora caninum has a possible role in causing abortions in sheep. Transmission of N. caninum via semen has been documented in cattle. This study aimed to investigate if horizontal transmission through semen was also possible in sheep. Initially, 6-month old crossbred ram lambs (n=32), seronegative to N. caninum, were divided into 4 equal groups. Group 1 remained uninoculated whilst the remainder were inoculated with N. caninum tachyzoites intravenously as follows: Group 2 - 50 tachyzoites; Group 3 - 10(3) tachyzoites; Group 4 - 10(7) tachyzoites. Semen samples were collected weekly for 8 weeks for the detection of N. caninum DNA and quantified using quantitative PCR (qPCR). Plasma collected 1 month post-inoculation was subjected to ELISA (IDEXX Chekit) and Western blot. At 2 weeks post-infection, three rams from Group 1 (uninoculated) and three rams from Group 4 (10(7)tachyzoites/ml) were mated with two groups of 16 ewes over two oestrus cycles. Ewe sera collected 1 and 2 months post-mating were tested for seroconversion by ELISA and Western blot. All experimentally infected rams seroconverted by 1 month with ELISA S/P% values ranging from 11% to 36.5% in Group 2, 12-39.5% in Group 3 and 40-81% in Group 4. However, none of the ewes mated with the experimentally infected rams seroconverted. For the Western blot, responses towards immunodominant antigens (IDAs) were observed in ram sera directed against proteins at 10, 17, 21, 25-29, 30, 31, 33 and 37 kDa. Rams in Group 2, 3 and 4 were noted to have at least 3 IDAs present. None of the ewes showed any of the 8 prominent IDAs except for the one at 21 kDa which was seen in 30 out of 32 ewes in both groups. N. caninum DNA was detected intermittently in the ram's semen up to 5 weeks post-inoculation with the concentrations ranging from that equivalent to 1-889 tachyzoites per ml of semen. Low concentrations of N. caninum DNA were also detected in the brain tissue of two rams (Groups 1 and 4). These results suggest that although N. caninum DNA can be found in the semen of experimentally infected rams, the transmission of N. caninum via natural mating is an unlikely event. PMID:23819894

Syed-Hussain, S S; Howe, L; Pomroy, W E; West, D M; Smith, S L; Williamson, N B

2013-11-01

201

EVALUATION OF CHROMOSOME BREAKAGE AND DNA INTEGRITY IN SPERM: AN INVESTIGATION OF REMOTE SEMEN COLLECTION CONDITIONS  

Science.gov (United States)

Home collection of ejaculated semen would facilitate participation rates and geographic diversity in reproductive epidemiology studies. Our study addressed concerns that home collection and overnight mail return might induce chromosome/DNA damage. We collected semen from 10 hea...

202

Is smoking a risk factor for decreased semen quality? : A cross-sectional analysis  

DEFF Research Database (Denmark)

Previous studies suggest a deleterious effect of cigarette smoking on semen quality, but their results have not been consistent. We studied the association between current smoking and semen characteristics and hormonal levels in a large group of healthy men.

Ramlau-Hansen, Cecilia; Thulstrup, A M

2007-01-01

203

Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry.  

Science.gov (United States)

The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O. mykiss during storage time and its relation to the spawning period are little known. This study was designed to evaluate the effects of in vitro short-term storage on sperm functional parameters in O. mykiss, determined by flow cytometry. Semen samples of the first spawning - undiluted (SSD) and diluted (SD) (Storfish(®) 1 : 2v/v; IMV AI solutions, France) - were stored at 4 °C for 14 days. Motility, viability (PMI: plasma membrane integrity) and mitochondrial membrane potential (??M) were assessed. On the fifth day of storage, spermatozoa showed a motility >70% (SSD: 78.3% versus SD 85.0%), PMI (81.5% SSD/87.2% SD) and ??M (72.5% SSD/SD 80.0%) (P semen. In conclusion, the results here provide new data on O. mykiss sperm quality with respect to in vitro short-term storage evaluated by flow cytometry. PMID:24717099

Trigo, P; Merino, O; Figueroa, E; Valdebenito, I; Sánchez, R; Risopatrón, J

2015-05-01

204

Semen quality: variations among fathers and effects of moderate alcohol drinking  

OpenAIRE

Semen analysis results from over 750 fathers in the USA demonstrated marked differences in the quality of semen from men at different locations and of different ethnic groups. Another paper failed to demonstrate any effects of moderate alcohol consumption during the week before provision of an ejaculate on semen quality and few on serum hormones, of over 8300 men in Europe and the USA. While these observations are interesting, the reasons for regional and ethnic differences in semen quality o...

Cooper, Trevor G.

2014-01-01

205

Effects of Diluents, Cryoprotectants, Equilibration Time and Thawing Temperature on Cryopreservation of Duck Semen  

OpenAIRE

A series of sequential experiments were carried out to determine optimum diluents, cryoprotectants, equilibration time, and thawing temperature for frozen duck semen in order to set up the commercial semen cryopreservating techniques which could be applied to the conservation of genetic resources, breeding, and commercial production in domestic ducks. In experiment 1, the seven semen extenders were studied to determine efficacy of the diluent on cryopreservation of duck Semen. The result show...

Han, X. F.; Niu, Z. Y.; Liu, F. Z.; Yang, C. S.

2005-01-01

206

Fertility of Cow in Using Locally Produced Chilled and Imported Frozen Semen  

OpenAIRE

The experiment was carried out at Central Cattle Breeding Station and Dairy farm, Savar, Dhaka, and 3 sub- station and 9 points of Chandpur District in Bangladesh to evaluate the quality and fertilizing capacity of locally produced chilled and imported frozen semen. Motility, sperm concentration and mass activity of semen from different experimental bulls were almost similar. Quality of imported frozen semen was better than that of locally produced chilled semen in respect of motility,...

Das, A. K.; Ali, M. Y.; Islam, M. A.; Hira, A. K.; Ali, S. Z.

2002-01-01

207

Relationships among frozen-thawed semen fertility, physical parameters, certain routine sperm characteristics and testosterone in breeding Murrah buffalo (Bubalus bubalis bulls  

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Full Text Available Aim: The present study was carried out to examine the relationships among frozen-thawed semen fertility, physical parameters, seminal quality, and testosterone concentration in Murrah buffalo bulls. Materials and Methods: A total of 30 breeding Murrah buffalo bulls (either progeny tested or under progeny testing program were randomly selected from two government bull farms in Punjab. None of the bulls selected for this study had any preceding physical abnormality. A field fertility trial was conducted to determine the first service conception rate (FSCR. The number of females inseminated per bull semen was 10. All the bulls were inspected for structural soundness, measurement of scrotal circumference, testicular biometry, and internal pelvic area (IPA. Frozen-thawed semen was evaluated for total motility, progressive motility, viability, concentration, abnormality, and hypo-osmotic swelling test (HOST. Testosterone was estimated in blood plasma, seminal plasma as well as frozen-thawed semen extracts for establishing relationship. Results: The FSCR was 48% in the bulls having a scrotal circumference of ?44 cm, although, there was no significant correlation between FSCR and scrotal circumference. Similarly, no consistent relationship existed between sperm concentration and scrotal circumference. A positive correlation was observed between IPA and FSCR (r=0.294. Of the six post-thaw seminal components (total motility, progressive motility, viability, HOST (%, total abnormality and concentration only total motility had a high significant (p<0.01 correlation with FSCR (r=0.694. Varied correlations existed between other seminal parameters and fertility. Using a simple regression analysis, the post-thaw motility, IPA, prepuce length and testosterone (independent variables combined to explain approximately 62% of the variation in the FSCR (dependent variable. Conclusion: The present study indicated that despite low to high correlations between seminal characteristics, physical parameters, fertility, and testosterone; the observations support the importance of these components and their function in maintaining semen quality and subsequent fertility.

A. K. Singh

2014-09-01

208

Parental age at delivery and a man's semen quality  

DEFF Research Database (Denmark)

STUDY QUESTION: Is parental age at delivery associated with a man's semen quality? In this large register-based study both mother's and father's age are found to have minimal effects on semen quality in men. BACKGROUND: Both maternal and paternal age have been associated with a range of adverse health effects in the offspring. Given the varied health effects of parental age upon offspring, and the sensitivity of genital development to external factors, it is plausible that the age of a man's mother and father at conception may impact his reproductive health. To our knowledge this is the first examination of the effects of parental age on semen quality. METHODS: The study was based on Danish men referred to the Copenhagen Sperm Analysis Laboratory due to infertility in their partnership. Men born from 1960 and delivering a semen sample until year 2000 were included. The men were linked to the Danish Civil Registration System to obtain information on parent's age at delivery. Logistic regression analyses were used to calculate odds ratios and 95% confidence intervals for impaired semen quality. Linear regression analyses were used to examine a relationship between semen parameters and paternal age. RESULTS: There were no convincing effect of either mother's or father's age on a man's semen quality. As no trends were noted, the few statistically significant results are likely attributable to chance. LIMITATIONS, REASONS FOR CAUTION: Information regarding individual subject characteristics which may impact sperm production (i.e. smoking, BMI) were not available. While our sample size was large, we cannot exclude the possibility that a trend may have been identified with a still larger sample. In addition, the Danish Civil Registration System is merely administrative and hence does not discriminate between biological and adopted children. However, the low rate of adoption (?2%) suggests that misclassification would have a minimal impact. The men were all referred to the laboratory for infertility problems in their partnership and, therefore, do not represent the general population. We, however, compared semen quality among men within the cohort, and it is therefore less important whether they, in fact, represent the general population. WIDER IMPLICATIONS OF THE FINDINGS: The current study found no link between parental age and a son's semen quality, suggesting other factors may explain recent impairments in men's reproductive health. STUDY FUNDING: This work was supported by the Hans and Nora Buchard's Fund and the Kirsten and Freddy Johansen's Fund.

Priskorn, Lærke; Jensen, Tina K

2014-01-01

209

Occupational exposure to pesticides and consequences on male semen and fertility: a review.  

Science.gov (United States)

Exposure to pesticides affects many body organs including reproductive system. Disorder of the reproductive system leads to infertility and therefore has been in the center of attention within the recent decades. Pesticides are one of the compounds that might reduce the semen quality in the exposed workers according to current knowledge. Although many underlying mechanisms have been proposed, the mechanisms of action are not clarified yet. The object of the present review was to criticize all the results of studies which evaluated the pesticide effects on male reproductive system. Results indicate that semen changes are multifactorial in the workers exposed to pesticides as there are numerous factors affecting sperm quality in occupational exposures. Majority of pesticides including organophosphoruses affect the male reproductive system by mechanisms such as reduction of sperm density and motility, inhibition of spermatogenesis, reduction of testis weights, reduction of sperm counts, motility, viability and density, and inducing sperm DNA damage, and increasing abnormal sperm morphology. Reduced weight of testes, epididymis, seminal vesicle, and ventral prostate, seminiferous tubule degeneration, change in plasma levels of testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH), decreased level and activity of the antioxidant enzymes in testes, and inhibited testicular steroidogenesis are other possible mechanisms. Moreover, DDT and its metabolites have estrogenic effects on males. Although effect of pesticides on sperm quality is undeniable, well-designed long-term studies are needed to elucidate all the possible affecting variables such as socioeconomic, cultural, nutritional, occupational, physical, and clinical characteristics alongside pesticides. PMID:24487096

Mehrpour, Omid; Karrari, Parissa; Zamani, Nasim; Tsatsakis, Aristides M; Abdollahi, Mohammad

2014-10-15

210

Semenogelin, the main protein of the human semen coagulum, regulates sperm function.  

Science.gov (United States)

Semenogelin (Sg), the main component of the human semen coagulum, is an important and versatile protein acting on several sperm parameters, both as intact or degraded Sg. Sg originates mostly from seminal vesicle and probably is responsible for sperm immobilization in the seminal coagulum. Purified Sg can be cross-linked by transglutaminase or phosphorylated by kinases, but the actual occurrence of these reactions in reproductive physiology is not clear. Experimental evidence demonstrates that prostate-specific antigen (PSA) rapidly cleaves Sg, an event temporally associated with semen liquefaction and initiation of sperm motility. Sg and its degradation peptides participate in various processes including Zn +2 shuttling, antibacterial activity, hyaluronidase activation, and so on. Sg inhibits sperm motility at the concentration found in the coagulum, but the rapid processing by PSA allows initiation of movement. The mechanism of Sg action and its targets are not known, but improper Sg degradation decreases fertility. Sg and its degradation peptides block sperm capacitation and associated events at concentrations much lower than those of seminal plasma and could play important role in preventing premature capacitation. The effects of Sg are dependent on time and proteolysis due to PSA, and any imbalance may affect sperm physiology and fertility. PMID:17253191

de Lamirande, Eve

2007-02-01

211

Decreased levels of genuine large free hCG alpha in men presenting with abnormal semen analysis  

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Full Text Available Abstract Background The pregnancy hormone human chorionic gonadotropin (hCG and its free subunits (hCG alpha, hCG beta are produced in the male reproductive tract and found in high concentrations in seminal fluid, in particular hCG alpha. This study aimed to elucidate changes in peptide hormone profiles in patients showing abnormal semen analyses and to determine the genuineness of the highly abundant hCG alpha. Methods Seminal plasma was obtained from 45 male patients undergoing semen analysis during infertility workups. Comprehensive peptide hormone profiles were established by a panel of immunofluorometric assays for hCG, hCG alpha, hCG beta and its metabolite hCG beta core fragment, placental lactogen, growth hormone and prolactin in seminal plasma of patients with abnormal semen analysis results (n = 29 versus normozoospermic men (n = 16. The molecular identity of large hyperglycosylated hCG alpha was analyzed by mass-spectrometry and selective deglycosylation. Results hCG alpha levels were found to be significantly lower in men with impaired semen quality (1346 +/- 191 vs. 2753 +/- 533 ng/ml, P = 0.022. Moreover, patients with reduced sperm count had reduced intact hCG levels compared with normozoospermic men (0.097 +/- 0.022 vs. 0.203 +/- 0.040 ng/ml, P = 0.028. Using mass-spectrometry, the biochemical identity of hCG alpha purified from seminal plasma was verified. Under non-reducing conditions in SDS-PAGE, hCG alpha isolated from seminal plasma migrated in a manner comparable with large free hCG alpha with an apparent molecular mass (Mr, app of 24 kDa, while hCG alpha dissociated from pregnancy-derived holo-hCG migrated at approximately 22 kDa. After deglycosylation with PNGase F under denaturing conditions, all hCG alpha variants showed an Mr, app of 15 kDa, indicating identical amino acid backbones. Conclusions The findings indicate a pathophysiological relevance of hCG, particularly its free alpha subunit, in spermatogenesis. The alternative glycosylation pattern on the free large hCG alpha in seminal plasma might reflect a modified function of this subunit in the male reproductive tract.

Plas Eugen

2011-08-01

212

Correlation between lead and cadmium concentration and semen quality.  

Science.gov (United States)

There are contrary reports of association of lead and cadmium with the decline in semen quality. This study evaluates whether seminal lead (Pb) and cadmium (Cd) at environmental concentration are associated with altered semen quality. We conducted a study of healthy fertile and infertile men 20-43 years of age attending the Andrology Laboratory of Reproductive Biology Department for semen analysis. The semen analysis was carried out according to the WHO 2010 guidelines. Seminal lead and cadmium were estimated by ICP-AES. The lead and cadmium values were significantly higher in infertile subjects. A negative association between seminal lead or cadmium concentration and sperm concentration, sperm motility and per cent abnormal spermatozoa was found. This study shows that exposure to Pb (5.29-7.25 ?g dl(-1) ) and cadmium (4.07-5.92 ?g dl(-1) ) might affect semen profile in men. Age, diet, smoking and tobacco chewing habits may have an influence on the increase in exposure to Pb and Cd in the individual subjects. PMID:25228328

Pant, N; Kumar, G; Upadhyay, A D; Gupta, Y K; Chaturvedi, P K

2014-09-16

213

Lipid concentrations and semen quality: the LIFE study.  

Science.gov (United States)

The decline in sperm count rates over the last 50 years appears to parallel the rising prevalence of obesity. As lipid levels are strongly associated with obesity, high lipids levels or hyperlipidaemia may thus play an important role in the decline in fertility in addition to other environmental or lifestyle factors. The objective of this population based cohort study was to evaluate the association between men's serum lipid concentrations and semen quality parameters among 501 male partners of couples desiring pregnancy and discontinuing contraception. Each participant provided prospectively up to two semen samples (94% of men provided one or more semen samples, and 77% of men provided a second sample approximately 1 month later). Linear mixed effects models were used to estimate the associations between baseline lipid concentrations and semen quality parameters, adjusted for age, body mass index and race. We found that higher levels of serum total cholesterol, free cholesterol and phospholipids were associated with a significantly lower percentage of spermatozoa with intact acrosome and smaller sperm head area and perimeter. Our results suggest that lipid concentrations may affect semen parameters, specifically sperm head morphology, highlighting the importance of cholesterol and lipid homeostasis for male fecundity. PMID:24596332

Schisterman, E F; Mumford, S L; Chen, Z; Browne, R W; Boyd Barr, D; Kim, S; Buck Louis, G M

2014-05-01

214

Semen and the diagnosis of infertility in Aristotle.  

Science.gov (United States)

Aristotle (384-322bc) was one of the leading intellectual figures of all time. In his work he systematised a massive amount of knowledge on a diverse range of subjects, including medicine. This article discusses the observations and hypotheses of this great philosopher on semen and infertility, as they are presented in his work Generation of Animals. This is combined with an evaluation of his positions in relation to those of the Hippocratic Corpus on the same subject. An extensive review of Aristotle's work Generation of Animals was performed with particular focus on his perspectives about semen and infertility. Publications referring to this work were also reviewed. According to Aristotle, semen is that which contains the principles that come from both parents when they unite. He believed that semen was formed by the secretion of nutriments by the body, developing his theories of sterility on this basic principle. A lack of fertility is attributed to genetic or acquired causes. He proposed methods for diagnosing sterility, primarily the 'water test' for men and the 'pessary' method for women. Even if his observations contain clear mistakes, such as attributing only secondary functions to male testicles and the identification of menses as women's 'seed', Aristotle's views also contain keen observations and exceptional thinking, both on the characteristics of semen and the causes of sterility (infertility). PMID:17212808

Trompoukis, C; Kalaitzis, C; Giannakopoulos, S; Sofikitis, N; Touloupidis, S

2007-02-01

215

Metil-formamida na criopreservação de sêmen ovino / Methyl-formamide in ram semen cryopreservation  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Objetivou-se avaliar a eficácia da metil-formamida na criopreservação do sêmen ovino. O pool de sêmen utilizado no experimento foi obtido a partir da coleta, com vagina artificial, do sêmen de quatro carneiros mestiços Santa Inês, com idade aproximada de quatro anos. As coletas foram realizadas uma [...] vez por semana, por seis semanas consecutivas, correspondendo, cada semana, a uma repetição do experimento. As frações do pool foram diluídas em cinco diferentes meios de congelação: (1) tris-gema com 5,3% de glicerol (TG5,3G); (2) tris-gema com 3% de metil-formamida (TG3MF); (3) tris-gema com 5% de metilformamida (TG5MF); (4) tris-gema com 7% de metil-formamida (TG7MF); (5) tris-gema com 9% de metil-formamida (TG9MF). Foram avaliadas a motilidade progressiva e o vigor das células espermáticas e realizado o teste de termorresistência pós-descongelação. O tratamento que obteve maior motilidade foi o TG5,3G (50%), seguido do TG3MF (38%) e os tratamentos que apresentaram menor motilidade progressiva foram TG5MF (29%), TG7MF (1,0%), TG9MF (6,0%). Os meios contendo metil-formamida apresentaram resultados inferiores ao meio controle para preservar a integridade morfológica dos espermatozoides, sendo que nos meios TG7MF e TG9MF menos de 60% de espermatozóides apresentaram-se morfologicamente normais. Os espermatozoides do meio TG5,3G apresentaram motilidade (15%) e vigor (2,8) similares aos do meio TG3MF (15% e 2,6, respectivamente) no teste de termorresistência, mas o meio TG5,3G preservou melhor a integridade funcional da membrana plasmática. O glicerol foi mais eficiente como crioprotetor do que a metil-formamida na criopreservação de sêmen ovino. Abstract in english The aim of this study was to evaluate the efficacy of methyl-formamide in ram semen cryopreservation. The semen pool used in this experiment was obtained by artificial vagina collection from four mixed breed Santa Inês rams, around four years of age. Semen collection was performed once a week, durin [...] g six weeks. Each week corresponded to one experiment replication. The semen pool was divided in five fractions in order to be diluted in one of the following freezing media: (1) tris-egg yolk with 5.3% of glycerol (TG5.3G); (2) tris-egg yolk with 3% of methyl-formamide (TG3MF); tris-egg yolk with 5% of methyl-formamide (TG5MF); tris-egg yolk with 7% of methyl-formamide (TG7MF); tris-egg yolk with 9% of methyl-formamide (TG9MF). Semen progressive motility, vigor and thermoresistance were evaluated. The treatments TG5.3G (50%) and TG3MF (38%) showed higher progressive motility after thawing, while TG5MF (29%), TG7MF (1%) and TG9MF (6%) showerd lower motility. Freezing media containing methyl-formamide were less effective in preserving spermatozoa membrane integrity and morphology than control media. In TG7MF and TG9MF extenders, less than 60% spermatozoa showed normal morphology. After thermoresistance test, semen cryopreserved in TG3MF showed vigor (2.6) and motility (15%) statistically similar to TG5.3G media (15% and 2.8, respectively); however, the extender TG5.3G was more effective in preserving plasma membrane functional integrity. In conclusion, in the experimental conditions used, glycerol showed more cryoprotectant potential than methyl-formamide.

Carlos Pereira das, Graças; Alexandre In Piao Gomes, Lim; Andrei Antonioni Guedes, Fidelis; Júlio Roquete, Cardoso; Hélio, Blume; Rafael Gianella, Mondadori.

2013-12-01

216

Rooster Semen Cryopreservation: Effect of Pedigree Line and Male Age on Post-Thaw Sperm Function  

Science.gov (United States)

The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in preservation of commercial genetic stocks. Our objective was to evaluate the cryosurvival of semen from 8 pedigreed layer lines at the onset and end of production. Semen from 160 roosters (20/line) was...

217

Rooster Semen Cryopreservation: Effect of Line Genetics and Male Age on Sperm Function  

Science.gov (United States)

The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in commercial production or preservation of genetic stocks. Our objective was to evaluate the cryosurvival of semen from 8 pedigreed layer lines at the onset and end of production. Semen from 160 roosters...

218

Effect of cushioned or single layer semen centrifugation before sex sorting on frozen stallion semen quality.  

Science.gov (United States)

The aim of this study was to compare the effect of presorting centrifugation (cushioned [CC] or single-layer colloid [SLC]), with simple dilution (SD), on the quality of sex-sorted stallion semen before and after sorting and after freezing and thawing. Four ejaculates from each of two fertile stallions were collected 1 week apart and evaluated for percent total sperm motility (TM), percent viable acrosome-intact sperm (VAI), and DNA quality (percentage of DNA fragmentation index). Freezing caused, independently from CC and SLC treatments, a significant decrease of TM (P < 0.05) and VAI (P < 0.05) in both unsorted and sorted semen. On the other hand, sorting did not impair TM and VAI and, interestingly, improved DNA quality in all treatments only before freezing (28 vs 13, 28 vs 10, 22 vs 7 in SD, CC, and SLC for unsorted vs sorted groups, respectively; P < 0.05); this positive effect was lost in the same samples after freezing and thawing, suggesting that the freezing process reduces the DNA quality of sex-sorted sperm. Our results suggest that CC and SLC are not able to select those spermatozoa that possess a better ability to withstand sperm processing associated with sperm sorting and freezing. PMID:25542457

Mari, G; Bucci, D; Love, C C; Mislei, B; Rizzato, G; Giaretta, E; Merlo, B; Spinaci, M

2015-04-01

219

Effects of Seasonal Changes and Shearing on Thermoregulation, Blood Constituents and Semen Characteristics of Desert Rams (Ovis aries  

Directory of Open Access Journals (Sweden)

Full Text Available This experiment was designed to study the effects of shearing in different seasons (winter vs. summer on thermoregulation, blood parameters and semen characteristics of desert rams. Eight intact healthy rams were randomly assigned into two groups (n = 4. The control group was kept unshorn (UN with intact pelage, the mean length of hair left was approximately 1.5 cm and the treated group was shorn (SH. Rectal temperature (Tr and Respiration Rate (RR measurements were carried out twice daily throughout the experimental period. Blood samples were collected once weekly for the evaluation of Packed Cell Volume (PCV, Total (TLC and Differential (DLC leukocyte count, Serum Total Protein (STP, Serum Albumin (SA, Serum Urea (SU and Plasma Glucose (PG concentration. Semen samples were collected once weekly for the determination of Ejaculate Volume (EV, Sperm Mass (SM and individual (SIM motility, Sperm Cell Concentration (SCC, live (LSP and abnormal (ABS sperm percent and semen pH. Scrotal Circumference (SC measurements were performed weekly. Shearing of desert rams significantly lowered the morning Tr in both seasons and the afternoon Tr during summer ,while RR was significantly lower in both seasons in the afternoon. The PCV was significantly lower in shorn rams during summer compared to winter and PG was significantly higher during winter compared to summer. In both seasons shearing significantly lowered SIM. It is concluded that shearing significantly affected thermoregulation, blood composition and semen characteristics during winter and summer. It is concluded that shearing in different season significantly affected thermoregulation, blood parameters and seminal traits of Desert Hamari rams.

M. Abdelatif Abdalla

2013-01-01

220

Effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey semen.  

Science.gov (United States)

The aim of this work was to study the effects of dilution and centrifugation (i.e., two methods of reducing the influence of the seminal plasma) on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey (Equus asinus) semen. Fifty ejaculates from nine Catalonian jackasses were collected. Gel-free semen was diluted 1:1, 1:5 or 1:10 with Kenney extender. Another sample of semen was diluted 1:5, centrifuged, and then resuspended with Kenney extender until a final dilution of 25x10(6) sperm/ml was achieved (C). After 24 h, 48 h or 72 h of refrigerated storage at 5 degrees C, aliquots of these semen samples were incubated at 37 degrees C for 5 min. The percentage of viable sperm was determined by staining with eosin-nigrosin. The motility characteristics of the spermatozoa were examined using the CASA system (Microptic, Barcelona, Spain). At 24h, more surviving spermatozoa were seen in the more diluted and in the centrifuged semen samples (1:1 48.71%; 1:5 56.58%, 1:10 62.65%; C 72.40%). These differences were maintained at 48 h (1:1 34.31%, 1:5 40.56%, 1:10 48.52%, C 66.30%). After 72 h, only the C samples showed a survival rate of above 25%. The four known donkey motile sperm subpopulations were maintained by refrigeration. However, the percentage of motile sperms in each subpopulation changed with dilution. Only the centrifuged samples, and only at 24h, showed exactly the same motile sperm subpopulation proportions as recorded for fresh sperm. However, the 1:10 dilutions at 24 and 48 h, and the centrifuged semen at 48 h, showed few variations compared to fresh sperm. These results show that the elimination of seminal plasma increases the survival of spermatozoa and the maintenance of motility patterns. The initial sperm concentration had a significant (P<0.05) influence on centrifugation efficacy, but did not influence the number of spermatozoa damaged by centrifugation. In contrast, the percentage of live spermatozoa in the fresh semen significantly influenced the number of spermatozoa damaged by centrifugation, but not centrifugation efficacy. PMID:19747718

Miró, J; Taberner, E; Rivera, M; Peña, A; Medrano, A; Rigau, T; Peñalba, A

2009-11-01

221

Seminal plasma stimulates cytokine production in endometrial epithelial cell cultures independently of the presence of leucocytes.  

Science.gov (United States)

We examined the effects of normal and leucocyte-positive semen on cytokine expression in endometrial epithelial cell cultures. Cytokines in pooled seminal plasma (SP) samples of men with normal semen parameters without (n = 9) and with leucocytospermia (LCS) (n = 9) were determined. Cultures of epithelial endometrial cells of women (n = 7) in the secretory phase were incubated with 10% SP for 24 h. Cytokines in culture supernatants and mRNA concentrations in the cultured cells were determined. Mean concentrations of interleukin (IL)-1beta and transforming growth factor (TGF)-beta1 were significantly higher (P semen parameters and LCS compared with control. Accordingly, a significant increase (P semen parameters were 24-fold (P semen. PMID:19032686

Ochsenkühn, R; Toth, B; Nieschlag, E; Artman, E; Friese, K; Thaler, C J

2008-12-01

222

Evaluation of semen parameters in semen donors in a ten-year period in the city of São Paulo  

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Full Text Available Objective: To evaluate sperm concentration, morphology and motility of Brazilian semen donors from 1992 to 2003, in the city of São Paulo. Methods: Retrospective study analyzing 182 donor semen samples from 1992 to 2003. The first and the second donated sample were analyzed for each donor. Donor average age was 30.8 years. Means with standard errors, medians with minimum and maximum values, and interquartile ranges were calculated for age, sperm concentration, semen volume, oval morphology and motility. The relation between each characteristic of the semen samples and the year of donation, as well as donor age and season of the year were studied by linear and multiple regression analysis. Results: Linear regression analysis showed that the sperm concentration (R2 = 19.1%, R2 = 20.2%, p < 0.0001 respectively and the oval morphology (R2 = 13%; R2 = 13.5%; p < 0.0001, respectively decreased significantly, even when the first or the second sperm collection is considered. The ejaculated volume showed slight increase during the period for both samples (R2 = 2.2%, p = 0.048; R-sq = 2.4%. p = 0.038, respectively. All characteristics did not depend on the donors’ age or season of the year when the samples were obtained. Conclusions: There was a decrease in spermatic concentration and percentage of oval sperm of semen donors samples from 1992 to 2003, in the city of São Paulo.

Sidney Glina

2010-12-01

223

Karakteristik Semen Segar dan Kualitas Semen Cair Kuda dalam Pengencer Dimitropoulos yang Disuplementasi dengan Fruktosa, Trehalosa dan Rafinosa  

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Full Text Available The objective of the experiment was to study the characteristics of stallion fresh semen and the quality of sperm preserved in Dimitropoulos extender (DV supplemented with different concentration of fructose, trehalose and raffinose. Semen were collected using artificial vagina from three stallions. Semen characteristics and quality were evaluated macro- and microscopically. Prior to extension, semen were centrifugated at 3000 rpm for 20 minutes. The condensed sperm were re-suspended in DV supplemented with different types of carbohydrate to meet the concentration of 200 million spz/ml. All samples were stored at room and chilled temperature, and were evaluated for motility and viability every 3 h and 12 h. The results of the experiments indicated that fresh semen characteristics were fair good; the volume, consistency, motility, live-dead ratio, concentration (106/ml, total spermatozoa (109/ejaculate and abnormality were 29.25±9.33 ml, watery, 7.00±0.12, 67.08±9.08%, 77.89±6.46%, 211.88±21.15, 6.28±2.45 and 27.26±4.64%, respectively. The supplementation of different type and concentration of carbohydrates did not significantly affect the motility and viability. However, the supplementation of 50 mM fructose significantly increased the motility and viability of the sperm compared to the control. In conclusion, carbohydrate supplementation in DV may not maintain the sperm quality, particularly in the medium with the osmolarity higher than 400 mOsm/kg.

Yudi

2007-12-01

224

Colloid centrifugation of fresh stallion semen before cryopreservation decreased microorganism load of frozen-thawed semen without affecting seminal kinetics.  

Science.gov (United States)

Freezability of equine semen may be influenced by microorganism population of semen. The objective of this study was to verify the effect of single-layer density gradient centrifugation (SLC) of fresh semen before cryopreservation on semen's microbial load (ML) and sperm cells kinetics after freezing-thawing. For that, one ejaculate was collected from 20 healthy stallions and split into control (C) samples (cryopreserved without previous SLC) and SLC samples (subjected to SLC). Semen cryopreservation was performed according to the same protocol in both groups. Microbial load of each microorganism species and total microbial load (TML) expressed in colony-forming units (CFU/mL) as well as frozen-thawed sperm kinetics were assessed in both groups. Additional analysis of the TML was performed, subdividing the frozen-thawed samples in "suitable" (total motility ? 30%) and "unsuitable" (total motility samples had less (P samples (155.69 × 10(2) ± 48.85 × 10(2) CFU/mL), mainly due to a reduction of Enterococcus spp. and Bacillus spp. A relationship between post-thaw motility and SLC effect on ML was noted, as only in samples with more than 30% total motility was ML reduced (P sperm cells was negligible. PMID:25287189

Guimarães, T; Lopes, G; Pinto, M; Silva, E; Miranda, C; Correia, M J; Damásio, L; Thompson, G; Rocha, A

2015-01-15

225

Sperm banking for male cancer patients: social and semen profiles  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english PURPOSE: Report the characteristics of cryopreserved semen from a cohort of male cancer patients, attitudes towards cryopreservation and outcomes of semen samples based on a 12-year cryopreservation program. MATERIAL AND METHODS: Data from 98 male cancer patients whose sperm samples were banked were [...] evaluated. Demographic parameters, semen characteristics, destination of sperm banked samples and questionnaires answered by the patients regarding cryopreservation time were evaluated. RESULTS: The cancer diagnoses were testicle (56.1%), prostate (15.3%), Hodgkin’s lymphomas (9.2%), non-Hodgkin’s lymphomas (7.1%), leukemia (3.1%) and other malignancies (9.2%). The patients with testicular cancer presented lower sperm concentration (p

Tatiana C.S., Bonetti; Fabio F., Pasqualotto; Priscila, Queiroz; Assumpto, Iaconelli Jr.; Edson, Borges Jr..

2009-04-01

226

The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies  

Energy Technology Data Exchange (ETDEWEB)

A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC). Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC), continuous use of sexed semen only (SS), utilization of sexed semen for both the first and second services with conventional semen afterwards (S2), and utilization of sexed semen for the first service with conventional semen afterwards (S1). Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer herd. (Author)

Joezy-Shekalgorabi, S.; Shadparvar, A. A.; Vries, A. de; Gay, K. D.

2014-06-01

227

Effects of discontinuous Percoll gradient centrifugation on the quality of bovine spermatozoa evaluated with computer-assisted semen analysis and fluorescent probes association.  

Science.gov (United States)

The objective of this study was to evaluate the quality of bovine frozen-thawed sperm cells after Percoll gradient centrifugation. Frozen semen doses were obtained from six bulls of different breeds, including three taurine and three Zebu animals. Four ejaculates per bull were evaluated before and after discontinuous Percoll gradient centrifugation. Sperm motility was assessed by computer-assisted semen analysis and the integrity of the plasma and acrosomal membranes, as well as mitochondrial function, were evaluated using a combination of fluorescent probes propidium iodide, fluorescein isothiocyanate-conjugated Pisum sativum agglutinin and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide. The procedure of Percoll gradient centrifugation increased the percentage of total and progressive sperm motility, beat frequency, rectilinear motility, linearity and rapidly moving cells. In addition, the percentage of cells with intact plasma membrane and mitochondrial membrane potential was increased in post-centrifugation samples. However, the percentage of sperm cells with intact acrosomal membrane was markedly reduced. The method used selected the motile cells with intact plasma membrane and higher mitochondrial functionality in frozen-thawed bull semen, but processing, centrifugation and/or the Percoll medium caused damage to the acrosomal membrane. PMID:21615453

Oliveira, L Z; Arruda, R P; Celeghini, E C C; de Andrade, A F C; Perini, A P; Resende, M V; Miguel, M C V; Lucio, A C; Hossepian de Lima, V F M

2012-02-01

228

Collection and characterization of semen in Mithun (Bos frontalis) bulls.  

Science.gov (United States)

The objective of this study was to collect semen from semiwild Mithun (Bos frontalis) bulls using an artificial vagina (AV) and to determine semen characteristics. Collection of semen with an AV was attempted in five Mithun bulls using both anestrous and estrous Mithun females. No Mithun bull mounted an anestrous female Mithun during 60 trials, but satisfactory mounting, including extension of the penis, occurred in 25 trials with estrous Mithun females. In 15 of these trials, semen was successfully collected in an AV with an internal temperature of 42 to 46 degrees C. However, in 10 trials with an AV with an internal temperature of 36 to 40 degrees C, semen was not collected. Mean (+/- SEM) intervals to first mount and to ejaculation in the AV were 27.9+/-3.6 sec and 113.8+/-6.6 sec, respectively. Semen volume and pH were 3.1+/-0.35 mL and 6.59+/-0.04, and mean mass activity (scale, 0 to 4), initial sperm motility, live sperm count, sperm concentration, total number of sperm in the ejaculate, and overall sperm length were 2.2+/-0.3, 78.6+/-2.6%, 80.7+/-2.2%, 710.8+/-66.8 x 10(6)/mL, 2114+/-364.4 sperm, and 67.9+/-0.6 microm, respectively. The proportion of morphologically normal sperm was 80.6+/-0.2%, whereas the proportion with a morphologically abnormal head, midpiece, tail, and acrosome were 4.2+/-0.4%, 1.6+/-0.5%, 6.1+/-1.1%, and 7.1+/-0.9%, respectively. The mean incidence of tail-less heads and proximal and distal protoplasmic droplets were 0.5+/-0.1%, 0.3+/-0.2%, and 2.4+/-0.3%, respectively. In conclusion, we successfully collected semen from semiwild Mithun bulls with an AV maintained at 42 to 46 degrees C, and overall, the semen was within the normal range of that collected from fertile domestic bulls. PMID:19589586

Bhattacharyya, H K; Goswami, B K; Bujarbaruah, K M; Deka, B C; Biswas, R K

2009-09-15

229

Effect of dilution temperature on boar semen quality.  

Science.gov (United States)

As boar semen is very sensitive to cold shock and changes in temperature during semen processing can have a profound impact on semen quality, the effect of the extender temperature at the time of dilution was investigated in a two-step dilution protocol for boar semen being processed for liquid storage. Fifteen boars of different breeds and ages from a commercial artificial insemination centre were included. One ejaculate per boar was collected and processed with Beltsville Thawing Solution semen extender. Each ejaculate was diluted (1 : 1) at 30 °C, and subsequently, the samples were diluted (30 × 10(6) sperm/ml) with either preheated extender [29.3 °C ± 0.2 °C, group A (GA)] or extender at room temperature [22.7 °C ± 0.6 °C, group B (GB)]. Samples were transported to the Faculty of Veterinary Medicine (University of Ghent, Belgium) in two isotherm boxes (one per group), stored at 17 °C and investigated for three consecutive days (D0 to D2). At D0, D1 and D2, motility parameters [computer-assisted semen analysis (CASA)] and the per cent of sperm with intact membrane (% IM) by eosin nigrosin staining were evaluated. At D0 and D2, the % of sperm with intact acrosome (% IA) was studied by Pisum sativum agglutinin staining. The average temperature of the 1 : 1 dilution was 29.4 °C ± 1.1 °C immediately after extender addition. No significant differences were found between groups for per cent motility [79.3 ± 9.0 for GA and 81.1 ± 9.2 for GB (p = 0.372)], % progressive motility [56.5 ± 13.3 for GA and 58.4 ± 13.8 for GB (p = 0.737)] or any CASA parameter. No differences were found for % IM [85.1 ± 10.7 and 84.5 ± 3.8 for GA and GB, respectively (p = 0.761)] and % IA [72.2 ± 9.4 for GA and 68.3 ± 16.6 for GB (p = 0.792)]. In conclusion, when a two-step dilution is performed, preheating the extender for the second dilution to match the semen temperature did not result in better semen quality compared to a dilution at a moderate room temperature. PMID:22066836

López Rodríguez, A; Rijsselaere, T; Vyt, P; Van Soom, A; Maes, D

2012-10-01

230

No increased sperm DNA fragmentation index in semen containing human papillomavirus or herpesvirus  

DEFF Research Database (Denmark)

It remains unknown whether human papillomaviruses (HPVs) or human herpesviruses (HHVs) in semen affect sperm DNA integrity. We investigated whether the presence of these viruses in semen was associated with an elevated sperm DNA fragmentation index. Semen from 76 sperm donors was examined by a PCR-based hybridization array that identifies all HHVs and 35 of the most common HPVs. Sperm DNA integrity was determined by the sperm chromatin structure assay. HPVs or HHVs, or both, were found in 57% of semen samples; however, sperm DNA fragmentation index was not increased in semen containing these viruses.

Kaspersen, Maja DØvling; Bungum, Mona Berger Håkonsen

2013-01-01

231

Semen banking: consideration on viral contamination in the era of new emerging viral infection  

OpenAIRE

To construct a semen bank, the collection of donated semen has to be done and an important concern is the safety of collected semen. The contamination is a big problem. Basically, the infectious pathogens can exist within donated semen, hence, a good donor screening is very important. Although viruses have an indirect role in sperm quality, but the evidence in banked semen is presently lack. This does not mean that there is no viral contamination but it might imply the inadequate concern on t...

Viroj Wiwanitkit

2011-01-01

232

The Relationship between Occupation and Semen Quality  

Directory of Open Access Journals (Sweden)

Full Text Available Background: Infertility can be a major concern for couples trying to conceive, and occupationalhazards may constitute a main cause of infertility in men. Studies conducted throughout the worldindicate that physical and chemical hazards in the workplace can have a negative impact on malefertility. The main objective of this study was to determine the frequency of occupational categoriesof men who attended an infertility clinic, and to evaluate the differences in the semen qualityparameters among occupational categories.Materials and Methods: This cross-sectional study was conducted on 1164 males who werereferred to the Infertility Research Center in Tehran for treatment of infertility in order to evaluatethe effects of certain occupations on infertility. The participants were divided into several categoriesaccording to their occupations and evaluated by means of a questionnaire for duration of infertility,BMI, sperm count, percentage of normal sperm morphology and percentages of sperm with class Aand class B motilities. Descriptive statistics, analysis of variance, and correlations were conductedusing SPSS 16.0 for Windows.Results: There were no statistically significant differences in the mean sperm count or spermmorphology between occupational categories. Assessment of the differences in the frequency ofsperm motility classes between occupational categories revealed a significant difference only inthe frequency of sperm with class B motility. The lowest mean percentages of sperm with class Bmotility were seen in those involved in the transportation industry, a finding in agreement with anumber of other researches.Conclusion: Our findings revealed an association between occupation and sperm motility. Sinceour study population was relatively small and in many cases exposures to work hazards were brief,a larger study group must be evaluated in order to support the preliminary results of this study.

Mohammad Hossein Vaziri

2011-01-01

233

Microscopic and flow cytometric semen assessment of Dutch AI-bucks : effect of semen processing procedures and their correlation to fertility  

OpenAIRE

This study was done to determine the effects of processing techniques on the quality of semen from Dutch AI-bucks with the view on improving pregnancy rates after artificial insemination (AI) with liquid or frozen–thawed semen. Motility of spermatozoa was estimated under a microscope whereas the percentage live spermatozoa and the percentage live spermatozoa with intact acrosomes were determined by means of flow cytometry. Aspects of semen processing that were investigated are storage tempe...

Peterson, K.; Kappen, M. A. P. M.; Ursem, P. J. F.; Nothling, Johan O.; Colenbrander, B.; Gadella, B. M.

2007-01-01

234

Seasonal variation in protein profiles and HSP70 of Holstein crossbred bull semen  

International Nuclear Information System (INIS)

Since HSP70 is the stress response protein, the impact of heat stress on semen quality may be displayed through the expression of protein profile and HSP70. This study investigated the seasonal effects on the protein profiles and HSP70 in spermatozoa and seminal plasma of 10 Holstein crossbred bulls from an AI centre located in Lopburi, Thailand. Bull semen was collected weekly for 8 consecutive weeks during rainy (average THI 79.34), cool (average THI 75.27), and summer (average THI 80.10) seasons. Protein was extracted from both spermatozoa and seminal plasma using Laemmli's sample buffer. The protein profiles of spermatozoa and seminal plasma were subjected to one-dimensional SDSPAGE with 12% (w/v) acrylamide gel and 4.0% (w/v) acrylamide stacking gel for 120 min. at 8 mA. To visualize the protein profiles, gels were fixed in acetic acid: ethanol: H2O (7: 40: 53), stained with 0.125% (w/v) Coomassie blue R-250 in acetic acid: ethanol: H2O (7: 40: 53) for 60 min., and distained with acetic acid: ethanol: H2O (11: 26: 63) until the background was clear. Western blotting, as described by Kamaruddin et al. was conducted to determine HSP70 using anti-HSP70 monoclonal antibody. Proteins in the polyacrylamide gel were electrophoretically transferred, for 90 min. at 156 mA, to a PVDF membrane. The membrane was rinsed in PBS and blocked overnight in a blocking solution (advanced ECL blocking; Amersham Life Science Inc., Oakville, ON, Canada)m Life Science Inc., Oakville, ON, Canada). The membrane was then incubated for 1 h at room temperature with monoclonal anti-HSP70 (H5147 Sigma Chemical Supplies CO., LTD), incubated with anti-mouse IgG horse radish peroxidase conjugated for 1 h at room temperature, and then detection for immunoreactive bands using ECL detection reagents (Amersham Life Science Inc.) on scientific imaging film. It was found that the profiles of protein were not different among seasons in both sperm and seminal plasma. The profiles of spermatozoa protein range from 10 to 220 kDa while most of proteins found in seminal plasma were low molecular weight (14-30 kDa). The HSP70 was found in both sperm and seminal plasma. However, the amount of HSP70 in winter appears to be greater compare to those found in summer and rainy seasons

235

New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen.  

Science.gov (United States)

Centrifugation of Apis mellifera L. drone semen is a necessary step in the homogenization of semen pools for the enlargement of the effective breeding population, as well as in the collection of semen by the so-called washing technique. It is also of interest for the removal of cryoprotectants after cryopreservation. The adoption of methods involving semen centrifugation has been hampered by their damaging effect to sperm. Here, we tested four new diluents as well as three additives (catalase, hen egg yolk, and a protease inhibitor), using sperm motility and dual fluorescent staining as indicators of semen quality. Three of the new diluents significantly reduced motility losses after centrifugation, as compared with the literature standard. Values of motility and propidium iodide negativity obtained with two of these diluents were not different from those measured with untreated semen. The least damaging diluent, a citrate-HEPES buffer containing trehalose, was then tested in an insemination experiment with centrifuged semen. Most queens receiving this semen produced normal brood, and the number of sperm reaching the storage organ of the queen was not significantly different from that in queens receiving untreated semen. These results could improve the acceptance of techniques involving the centrifugation of drone semen. The diluent used in the insemination experiment could also serve as semen extender for applications not involving centrifugation. PMID:24665683

Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

2014-02-01

236

Blood and Semen Selenium Concentrations and Semen Quality in Boars Fed Diets Supplemented with Organic or Inorganic Selenium  

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Full Text Available Effect of dietary supplementation of organic or inorganic selenium on blood and semen selenium concentrations and semen quality was determined in 10 boars. During the 4 weeks of pre-experimental period, all boars were fed a basal diet containing 0.15 mg kg-1 of inorganic selenium. Thereafter, all cows were randomly allocated into 2 groups of five boars which were fed a basal diet supplemented with either 0.3 mg kg-1 of inorganic selenium or 0.3 mg kg-1 of organic selenium for 84 days. Blood samples were collected from all boars to determine selenium concentrations at the end of pre-experimental period and at days 49 and 84 after supplementation. Semen samples were collected at the end of pre-experimental period and at days 35, 49, 63 and 84 to determine selenium concentrations and semen evaluation. For both inorganic and organic selenium groups, blood selenium concentrations at days 49 and 84 were higher than the concentration at day 0 and the concentrations did not differ between the two groups at all sampling periods. Semen selenium concentrations at days 35, 49, 63 and 84 were higher than the concentration at day 0 for both inorganic and organic selenium groups and the concentrations did not differ between the 2 groups at days 35, 49, 63 and 84. Sperm motility parameters including motility (%, progressive motility (%, Average Path velocity (VAP, ?m sec-1, Straight-line velocity (VSL, ?m sec-1 and Curvilinear velocity (VCL, ?m sec-1 did not differ between the 2 groups and among sampling periods. Results revealed that 0.3 mg kg-1 supplementation of either inorganic or organic selenium form in the basal diet containing 0.15 mg of selenium per kg could increase blood and semen selenium levels in the boars. With normally-fertile boars, both inorganic and organic form of selenium supplemented in the diet had similar effect on sperm motility characteristics in the boars.

Theera Rukkwamsuk

2012-01-01

237

Effects of Vitamin E Addition to Chicken Semen on Sperm Quality During in Vitro Storage of Semen  

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Full Text Available The purpose of this study was to evaluate the probable effects of the vitamin E addition in different levels to the extender of chicken semen on spermatozoa quality during storage of semen at 4°C for 0, 3, 6, 10 and 24 hours. Eight young Ross broiler breeder strain 308 roosters were used in this experiment. The collected semen from all roosters was mixed together and diluted with modified a Ringer’s solution. The diluted pooled semen was divided into 5 treatments (T. T1 was a control group without any vitamin E addition. For T2 to T5 groups 0.5 %, 1 %, 2 % and 3 % vitamin E (w/v, were added respectively. Treatments were evaluated for sperm motility, sperm viability and probable morphological defects after 0, 3, 6, 10 and 24 hours of incubation at 4°C. The evaluations of spermatozoa immediately after semen collection, were revealed no significant differences among values of treatment groups, whereas after incubating the treatments for different spans of time, the sperm progressive motility and viability rates for groups supplemented with vitamin E were significantly (P < 0.05 higher than that of the control group. In addition, morphological defect rates of chicken spermatozoa in the groups supplemented with different levels of vitamin E were significantly (P < 0.05 lower than that in control group. According to the results of this study we conclude that, the most excellent level of vitamin E for supplementation to the extended semen of chicken in order to improve the sperm motility and viability plus to reduce the morphological defect rates of the spermatozoa up to 24 hours storage time at 4°C is 2 % (w/v.

Saleh Tabatabaei

2011-06-01

238

Peptides released by physiological cleavage of semen coagulum proteins form amyloids that enhance HIV infection.  

Science.gov (United States)

Semen serves as a vehicle for HIV and promotes sexual transmission of the virus, which accounts for the majority of new HIV cases. The major component of semen is the coagulum, a viscous structure composed predominantly of spermatozoa and semenogelin proteins. Due to the activity of the semen protease PSA, the coagulum is liquefied and semenogelins are cleaved into smaller fragments. Here, we report that a subset of these semenogelin fragments form amyloid fibrils that greatly enhance HIV infection. Like SEVI, another amyloid fibril previously identified in semen, the semenogelin fibrils exhibit a cationic surface and enhance HIV virion attachment and entry. Whereas semen samples from healthy individuals greatly enhance HIV infection, semenogelin-deficient semen samples from patients with ejaculatory duct obstruction are completely deficient in enhancing activity. Semen thus harbors distinct amyloidogenic peptides derived from different precursor proteins that commonly enhance HIV infection and likely contribute to HIV transmission. PMID:22177559

Roan, Nadia R; Müller, Janis A; Liu, Haichuan; Chu, Simon; Arnold, Franziska; Stürzel, Christina M; Walther, Paul; Dong, Ming; Witkowska, H Ewa; Kirchhoff, Frank; Münch, Jan; Greene, Warner C

2011-12-15

239

Peptides Released by Physiological Cleavage of Semen Coagulum Proteins Form Amyloids that Enhance HIV Infection  

Science.gov (United States)

SUMMARY Semen serves as a vehicle for HIV and promotes sexual transmission of the virus, which accounts for the majority of new HIV cases. The major component of semen is the coagulum, a viscous structure composed predominantly of spermatozoa and semenogelin proteins. Due to the activity of the semen protease PSA, the coagulum is liquefied and semenogelins are cleaved into smaller fragments. Here, we report that a subset of these semenogelin fragments form amyloid fibrils that greatly enhance HIV infection. Like SEVI, another amyloid fibril previously identified in semen, the semenogelin fibrils exhibit a cationic surface and enhance HIV virion attachment and entry. Whereas semen samples from healthy individuals greatly enhance HIV infection, semenogelin-deficient semen samples from patients with ejaculatory duct obstruction are completely deficient in enhancing activity. Semen thus harbors distinct amyloidogenic peptides derived from different precursor proteins that commonly enhance HIV infection and likely contribute to HIV transmission. PMID:22177559

Roan, Nadia R.; Müller, Janis A.; Liu, Haichuan; Chu, Simon; Arnold, Franziska; Stürzel, Christina; Walther, Paul; Dong, Ming; Witkowska, H. Ewa; Kirchhoff, Frank; Münch, Jan; Greene, Warner C.

2011-01-01

240

The Optimum Semen Dilution for the Sperm Quality Index that is Most Predictive of Broiler Breeder Fertility  

OpenAIRE

The sperm quality index (SQI) can accurately predict overall semen quality and fertility when broiler breeder semen is diluted, at most, 10-fold prior to analysis. The objective of the present study was to determine if a lower semen dilution rate yields an SQI that is an even better predictor of semen quality and fertility when hens are inseminated with a constant volume of semen. Individual ejaculates from 28 males were analyzed for sperm concentration, viability, and the SQI prior to insemi...

Parker, H. M.; Mcdaniel, C. D.

2004-01-01

241

Cytokines release and oxidative status in semen samples from rabbits treated with bacterial lipopolysaccharide.  

Science.gov (United States)

This study was aimed to evaluate the effects of a lipopolysaccharide- (LPS) induced inflammation on cytokines release and oxidative status of semen samples from buck rabbits at different times after treatment. Semen analysis was performed by optical microscopy and sperm motility evaluation by the computer-assisted sperm analyzer. The presence of activated macrophages and apoptotic/necrotic sperm was evaluated by fluorescent microscopy. A panel of cytokines, interleukin (IL)-6, IL-8, IL-1?, and tumor necrosis factor-?, were detected and quantified in seminal plasma using the Bio-Plex Cytokine assay. Reactive oxygen metabolite and thiobarbituric acid-reactive substance determinations were carried out by spectrophotometry and tocopherol analysis by high performance liquid chromatography. The sperm motility and track speed were reduced in LPS-treated rabbits. The activated macrophages in LPS-treated buck rabbits significantly increased from 0.50 × 10(6)/mL (baseline) to 27 × 10(6)/mL on Day 21; successively, there was a progressive reduction. Apoptotic and necrotic sperm in LPS rabbits followed more or less the same trend. The reactive oxygen metabolite levels in semen from LPS-treated rabbits showed higher values compared with those evaluated in controls, particularly during the lag time, Days 1 to 3. The sperm thiobarbituric acid-reactive substances highlighted a peak in LPS-treated rabbits compared with those of controls on Day 1 after LPS treatment, and the different T isoforms (?, ?, and ?+?) showed a similar trend with a significant decrease on Day 1 after injection and a recovery on Days 30 to 56. Until Days 3 to 21 from the treatment, higher levels of IL-1? and tumor necrosis factor-? were detected in seminal plasma of LPS-treated rabbits. Interleukin-6 showed a peak on Day 3 after LPS treatment, and on Day 7, the value was similar to the control group. In conclusion, this study confirms that the buck rabbit is a good model for mimicking and understanding the inflammation mechanisms, which may induce male infertility, in particular that a systemic inflammatory status causes alterations to the sperm cells through a shift in the balance between the oxidant and antioxidant systems. PMID:25662201

Collodel, G; Moretti, E; Brecchia, G; Kuželová, L; Arruda, J; Mourvaki, E; Castellini, C

2015-04-15

242

Association between nitric oxide and 8-hydroxydeoxyguanosine levels in semen of diabetic men.  

Science.gov (United States)

The incidence of diabetes mellitus is rapidly increasing in the world. One of the complications of diabetes includes disturbance of the reproductive tract, such as infertility, erectile dysfunction, and endocrine disruption. Nitric oxide (NO) is a free radical produced by most cells including the human male and female reproductive tracts. NO has a dual role where low concentrations are essential for homeostatic cellular biology and physiology, but high levels have detrimental effects relating to cellular damage from this reactive oxygen species (ROS). 8-hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside of DNA that is currently used as a biomarker of cellular oxidative stress, where urinary levels can correlate with diabetic nephropathy and retinopathy. Our aim was to investigate the relationship between nitrate/nitrite levels and 8-OHdG levels in the semen of diabetic and non-diabetic men. Concentrations of nitrate/nitrite and 8-OHdG were examined in seminal plasma of 32 diabetic and 35 non-diabetic men. The level of nitrate/nitrite was assayed by colorimetric reaction and 8-OHdG was measured by ELISA. Our results showed that the seminal plasma nitrate/nitrite levels were significantly higher in the diabetic group (p?semen of diabetic men is suggestive of reactive oxygen species induced DNA damage that is correlated with 8-OHdG levels but not sperm parameters. These results support the further investigation of NO and 8-OHdG as biomarkers for assessing male infertility. PMID:22047525

Amiri, Iraj; Karimi, Jamshid; Piri, Hossein; Goodarzi, Mohammad Taghi; Tavilani, Heidar; Khodadadi, Iraj; Ghorbani, Marziye

2011-12-01

243

Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen  

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Full Text Available The objectives of the present study were to determine the effects of six different antibiotics in controlling the growth of semen contaminating bacteria and if these antibiotics have any adverse effect on Awassi ram spermatozoa. Semen samples from six mature Awassi rams were used in this study. A total number of 120 ejaculates were collected from the rams using an artificial vagina once a week. Semen ejaculates were evaluated for volume, sperm concentration, mass motility, individual motility, percentage live sperm, sperm abnormalities, and viable bacterial count. Semen samples were diluted by sodium citrate-fructose-egg yolk. The diluted semen sample was divided into 7 parts. Six types of antibiotics were added to the semen diluent parts including; penicillin G 1000 IU ml-1 with streptomycin 1 mg ml-1, gentamicin sulphate 250 mg ml-1, tetracycline 0.5 mg ml-1, lincomycin 1 mg ml-1, cefoperazone sodium 1mg ml-1, cefdinir 1 mg ml-1 and the seventh part considered as a control group without antibiotic addition. The diluted semen samples were cooled and preserved at 5 Co for 5 days. Cooled diluted semen samples were examined for individual motility, percent of live sperm, sperm abnormalities, acrosomal defects and bacterial count every 24 h until 5 days. Comparing with the control, all the antibiotics examined were effective in controlling bacterial growth (P<0.05 from 24 h to 96 h of preservation at 5 Co. Cefdinir and cefoperazone sodium proved to be significantly (P<0.05 effective than other antibiotics in controlling bacterial growth at 96 h of preservation as the bacterial count were 23.3 ± 3.7 x 103 / ml and 25.4 ± 6.2 x 103 / ml, respectively. Lincomycin, gentamicin sulphate and tetracycline proved ineffective in controlling bacterial growth at 96 h of preservation as the bacterial count were 57.1 ± 20.1 x 103 / ml, 52.5 ± 29.4 x 103 / ml and 46.5 ± 8.8 x 103 / ml, respectively. The addition of tetracycline to diluted ram semen significantly reduced (P<0.05 sperm individual motility and percent live sperm and a significant increase (P<0.05 acrosomal defects was observed at 96 h of preservation in comparison to control and other antibiotics. Sperm viability was highly correlated with bacterial count in the control part of diluted semen (r = 0.794; P < 0.01. It could be concluded from the results of the present study that additions of cephalosporins (cefdinir or Cefoperazone sodium at the dose of 1 mg ml-1 were most effective amongst the antibiotics used in checking the bacterial growth and improving semen quality of Awassi ram. [Vet. World 2012; 5(2.000: 75-79

O I Azawi

2012-04-01

244

EFFICACY OF IRON CHELATORS ON CAMPYLOBACTER CONCENTRATIONS IN TURKEY SEMEN  

Science.gov (United States)

Campylobacter is a leading bacterial cause of human food borne infections in the United States. Recent studies suggest that the organism is highly prevalent in poultry semen and may contribute to vertical transmission between the breeder hen and offspring. As Campylobacter requires iron for its gr...

245

TRIHALOMETHANE LEVELS IN HOME TAP WATER AND SEMEN QUALITY  

Science.gov (United States)

Trihalomethane Levels in Home Tap Water and Semen Quality Laura Fenster, 1 Kirsten Waller, 2 Gayle Windham, 1 Tanya Henneman, 2 Meredith Anderson, 2 Pauline Mendola, 3 James W. Overstreet, 4 Shanna H. Swan5 1California Department of Health Services, Division of Environm...

246

Evaluation of Bulls Based on Semen Quality and Herd Fertility  

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Full Text Available The data of the present study were collected from Bangladesh Milk Producers` Co-operative Union Limited (BMPCUL at Baghabarighat, Sirajgong, Bangladesh to evaluate the bull performance through semen quality and 30-day Non Return Rate (NRR. Data on 245 ejaculates and 12,750 services of almost same aged of 5 Sahiwal bulls (B1, B2, B3, B4 and B5 over a period of 5 years were collected from Animal Breeding Section and Cattle Feed Unit of BMPCUL. The significant (p<0.01 individual sire effect was found on semen volume, three stages of sperm motility (initial, on dilution and post-thawing and fertility. Significant (p>0.05 variation was not found for pH. The highest volume of semen per ejaculate, the initial, on dilution and post-thawing sperm motility were found in bull B4 and representing 5.60?0.19 mL, 73.56?0.61, 69.27?0.33 and 63.90?0.48%, respectively. The highest sperm motility was found in initial stage and then declined steadily up to post-thawing stage. Significant (p<0.01 positive correlations were observed between the three stages of sperm motility and fertility. The results of the present study revealed that evaluation of breeding bulls on the basis of semen quality and herd fertility is important and provides the guideline of the way to sire selection for reproductive performance.

M.P. Mostari

2004-01-01

247

Influence of the preservation temperature (37, 20, 4, -196°C) and the mixing of semen over sperm quality of Majorera bucks.  

Science.gov (United States)

This study assessed the effect of different semen storage temperatures and the influence of semen pooling in semen viability. In experiment 1, semen samples (n = 30) of five Majorera bucks were individually processed [Individual semen (IS)] and after the first dilution (Tris-yolk extender), semen-diluted aliquots from each male were pooled semen (PS). Thereafter, semen samples (IS and PS) were preserved as fresh semen (37 and 20°C), chilled semen (4°C) and frozen semen. Sperm motility and the percentage of abnormal sperm cells and intact membrane acrosomes were defined. Semen preservation at 20 and 4°C did not modify the quality of spermatozoa for the first 24 h, but the conservation at 37°C caused a dramatic fall in the semen motility from 12 h onwards. Furthermore, the longevity of frozen-thawed semen was limited to 4-6 h. No differences were observed in semen parameters when PS was compared with semen from individual males in any of the preservation protocols assessed. In experiment 2, 120 goats were distributed in four experimental groups: in group fresh individual semen (FIS, n = 30) and group frozen-thawed individual semen (FTIS, n = 30), does were transcervically inseminated with fresh semen and frozen-thawed semen from each individual male, respectively, and in group fresh pooled semen (FPS, n = 30) and group frozen-thawed pooled semen (FTPS, n = 30), goats were transcervically inseminated with FPS and FTPS, respectively. The kidding rate was very close in the FIS and FPS groups (70.0% and 73.7%, respectively), and no significant differences were observed in the fertility rate between FTIS and FTPS. The results of this study confirmed that semen samples may be preserved satisfactorily for 24 h both at 20 and 4°C. In addition, the mixture of semen of different bucks did not significantly modify the semen parameters when compared with semen from individual males. PMID:20565698

Batista, M; Niño, T; Santana, M; Alamo, D; Castro, N; Reyes, R; González, F; Cabrera, F; Gracia, A

2011-04-01

248

Effect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen  

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Full Text Available The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was diluted to a final concentration of 30 × 106 spermatozoa with citrate-egg yolk and in experiment 2; specimen was diluted with tris-egg yolk extender to the same concentration. In both experiments diluted semen was divided into three aliquots, including a control and two test groups. Each aliquot was rediluted with an equal volume of extender either without (control or with one of the antioxidants contained one of the following antioxidants: catalase (CAT; 100 IU mL-1 catalase (CAT; 200 IU mL-1 and control group. No significant differences were observed in sperm viability and motility following addition of catalase enzyme at concentration of 100 IU mL-1 and 200 IU mL-1 to citrate-egg yolk extender. But the highest sperm viability was achieved by addition of 100 IU mL-1 and 200 IU mL-1 catalase to tris-egg yolk semen extender compared with the control group (P < 0.05. Malondialdehyde levels did not change with addition of catalase in both extenders compared with the control group. The obtained results provide a new approach to the cryopreservation of bull semen, and could positively contribute to intensive cattle production.

Reza Asadpour

2011-11-01

249

Study on the effect of prostaglandin F2? treatment on semen characteristics and enzymatic activates of Awassi rams in breeding and non breeding seasons  

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Full Text Available The purpose of this research work was to determine the effects of PGF2?, given immediately before semen collection, on semen characteristics and libido in Awassi rams during breeding and non breeding season. The experiment was conducted in late summer to early autumn when major breeding activities commence and winter during the non breeding season at Mosul region in northern Iraq at the Animal Research and Practice Farm of the College of The Veterinary Medicine, University of Mosul. Twelve mature Awassi rams were used in this study. Animals were randomly allocated into two equal groups, the first group was administered 7.5 mg IM of PGF2?weekly and the second group as a control group received 1 ml of N-saline solution. Semen samples were collected from the Awassi rams 24 h after IM administration. Scrotal circumference (SC and testicular volume were measured weekly during the study period. Semen ejaculates were evaluated for semen volume, sperm concentration, sperm concentration/ejaculate, mass motility, individual motility, percentage live sperm, sperm abnormalities, and sperm acrosomal defects. Samples of seminal plasma were analyzed for the estimation of alanine amino transferase (ALT, aspartate amino transferase (AST, acid phosphatase (ACP, alkaline phosphatase (ALP and lactic dehydrogenase (LDH. Results of the present showed that PGF2? treatment to Awassi rams did not improve most semen characteristics in both breeding and non breeding seasons compared with the group. The only improvement of Awassi semen quality observed was in sperm concentration in the breeding season. The testicular volume showed a significant increase (P<0.05 in Awassi rams treated with PGF2? in breeding season compared to the control group and PGF2? treated group in the non breeding season. The mean activity of LDH enzyme estimated in the PGF2?treated group and control group showed a significant difference (P<0.05 between the two groups in the breeding season and non breeding season (52.34 ± 8.96 and 57.43 ± 19.9 vs. 117.02 ± 5.26 and 131.88 ± 5.01, respectively. Other enzymatic activities including ALT, AST, ACP and ALP showed no significant differences between Awassi rams treated with PGF2? and control groups in both breeding and non breeding seasons. In conclusion, PGF2?treatment of Awassi rams improved sperm concentration and testicular volume

Osama Ibrahim Azawi,

2011-05-01

250

Plasma membrane changes during the liquid storage of boar spermatozoa: a comparison of methods.  

Science.gov (United States)

Studies were performed on boar semen routinely used at the local artificial insemination (AI) centre. The semen was stored in a Safe Cell Plus commercial extender at 17 degrees C for nine days. The aim of our research was focused on changes in sperm plasma membrane integrity. The integrity of the sperm plasma membrane and acrosome as well as sperm motility decreased after dilution and during storage of the semen. The highest percentage of live sperm was identified by the eosin-nigrosin method, a lower percentage by the SYBR-14/PI test, and the lowest percentage of live cells was discovered by the hypoosmotic swelling (HOS) test (P < 0.01). There were significant differences between the results of staining methods and sperm motility (P < 0.01). No significant differences were found between the HOS test results and sperm motility. The plasma membrane integrity parameters positively correlated (P < 0.001) with each other and with sperm motility but negatively with aspartate aminotransferase activity. Our findings confirmed that the boar sperm aging changes, which increased during liquid semen preservation, were connected with the loss of function and integrity of the sperm plasma membrane. The employed complementary tests are comprehensive indicators of sperm membrane integrity during long-term semen preservation, and they can help establish the actual number of 'healthy' cells. The assays may be used in AI laboratories and should be incorporated into the routine of semen analysis. PMID:20159744

Gaczarzewicz, Dariusz; Piasecka, Ma?gorzata; Uda?a, Jan; B?aszczyk, Barbara; Stankiewicz, Tomasz; Laszczy?ska, Maria

2010-03-01

251

Inseminación artificial de alpacas con semen colectado por aspiración vaginal y vagina artificial / Artificial insemination of alpacas with semen collected by vaginal aspiration and by artificial vagina  

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Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Semen de alpaca fue colectado por dos métodos: por aspiración de la vagina de la hembra después de la monta natural y con vagina artificial. El semen colectado fue evaluado y diluido con Tris tamponado, y luego usado en inseminacion artificial. Se trabajó con 160 alpacas hembras adultas de capacidad [...] reproductiva comprobada y 5 alpacas machos. Se colectó semen post cópula de los cinco machos en 10 hembras, y se hicieron 50 colecciones de semen con vagina artificial de estos machos, dos veces por semana. Se determinó volumen, motilidad, concentración espermática, porcentaje de espermatozoides vivos, viscosidad y color. Los resultados para semen colectado por aspiración de la vagina y con vaginal artificial fueron: volumen (3.6 y 1.5 mL), motilidad (73.4 y 69.0%), concentración espermática (75.2 y 80.3 millones/mL), espermatozoides vivos (75.3 y 70.8%), respectivamente, con diferencia entre métodos (p Abstract in english Semen from alpacas was collected by two methods: by aspiration from the female’s vagina following mating and with an artificial vagina. Semen was collected, evaluated and extended with Tris buffer, and then used in artificial insemination. Altogether 160 female alpacas with proven reproductive histo [...] ry and five males were used. Semen was collected by vaginal aspiration from 10 females using five males as semen donors; likewise, semen from the same males was collected with an artificial vagina twice a week 50 times. Volume, motility, spermatic concentration, live spermatozoa, viscosity and color was evaluated. Seminal characteristics of semen collected by aspiration and with an artificial vagina were: volume (3.6 and 1.5 mL), motility (73.4 and 69.0%), sperm concentration (75.2 and 80.3 million/mL), live spermatozoa (75.3 and 70.8%) respectively, with statistical difference between methods (p

Virgilio, Alarcón B; Wilber, García V; P. Walter, Bravo.

252

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen.  

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The goal of this study was to develop a simple glucose-methanol extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen. Semen quality was assessed by determining post-thaw sperm motility and fertilizing ability at hatching stage. We tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30M) in a methanol extender on post-thaw sperm motility. Sperm motility parameters and fertilizing ability of semen cryopreserved in 0.1M glucose in 15% methanol (GM) were compared to previously described Tris-sucrose-KCl in 10% - methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 30min equilibration in GM extender before cryopreservation and 30min of post-thaw storage were determined. The beneficial effect of the glucose for semen cryopreservation was related to its concentration with a quite narrow optimum of 0.1 to -0.15M. The fertilization rates of frozen/thawed sperm were similar for both (TSKM and GM) tested extenders. The sperm motility and fertilization rate were not affected either by 30min equilibration in GM extender or by 30min of post-thaw storage. Our work indicates that the use a simple extender consisting of 0.1M glucose in 15% methanol can be an alternative cryopreservation method to those previously described for sturgeons. The use of an equilibration period and the possibility of post-thaw semen storage can improve organization of hatchery work and help with logistics of large-scale hatchery operations. PMID:25725469

Judycka, S; Szczepkowski, M; Ciereszko, A; Dietrich, G J

2015-04-01

253

Characterization and sorting of flow cytometric populations in human semen.  

Science.gov (United States)

Human semen is a complex biological matrix. It contains mature spermatozoa, immature germ cells, residual apoptotic bodies and, in some cases, epithelial cells and leucocytes. Hence, one of the challenges in applying flow cytometry in spermatology is the correct recognition of spermatozoa and their separation from signals of other semen cells/elements. In this study, we show that semen spermatozoa are included in a well-defined, flame-shaped FSC/SSC region (FR), by demonstrating that the count of the spermatozoa contained in such region overlaps that obtained by microscopy in the same samples. In FR, nuclear staining of semen samples reveals three different populations: unstained, brighter and dimmer. Unstained elements were previously characterized as apoptotic bodies of testis origin and the brighter elements represent the majority of semen spermatozoa, whereas the composition and the origin of the population with a lower nuclear staining is less clear, albeit we have previously shown that all the elements constituting it are positive for TUNEL. In this study, we sorted all the elements contained in FR region and demonstrated that the dimmer elements are spermatozoa. To further characterize dimmer spermatozoa, we evaluated apoptotic caspases and chromatin immaturity, the latter detected by aniline blue (AB) and chromomycin A (CMA3) staining. We found that caspases were much more expressed in the dimmer spermatozoa (71.4 ± 18.8%) than in the brighter (46.7 ± 15.1%), whereas similar amounts of spermatozoa with chromatin immaturity were found in both populations (brighter, AB: 48.2 ± 19.5%; CMA3: 48.5 ± 20.4% and dimmer, AB: 43.4 ± 19.8%; CMA3: 36.1 ± 18.0%). Hence, the role of apoptosis in generating dimmer spermatozoa and their DNA fragmentation appears clear, whereas the involvement of defects during the chromatin packaging remains elusive. PMID:24700807

Marchiani, S; Tamburrino, L; Olivito, B; Betti, L; Azzari, C; Forti, G; Baldi, E; Muratori, M

2014-05-01

254

The influence of boar breed and season on semen parameters  

Scientific Electronic Library Online (English)

Full Text Available SciELO South Africa | Language: English Abstract in english The aim of the present study was to demonstrate the influence of boar breed and season on semen parameters. The research material consisted of 31 boars: Polish Large White (PLW), Polish Landrace (PL), and Duroc x Pietrain (D x P), aged 8 to 24 months. The analysed material consisted of 1390 ejaculat [...] es, collected during the period January 2010 to October 2012. Semen samples were assessed in terms of semen volume (mL), sperm concentration (x 10(6) m/mL), total number of sperm (x 10(9)), total number of live sperm (x 10(9)) and number of insemination doses obtained from one ejaculate (n). In winter, an increase in sperm concentration was observed for the PLW breed. Moreover, an increase in the volume of semen produced for this breed was noted in summer and autumn. Differences between breeds for the total number of sperm and total number of live sperm were observed for the winter and spring periods. The largest semen volume was noted for the PLW breed (276.4 ± 9.66 mL). However, in the analysis of other sperm parameters, boars of this breed demonstrated the poorest results. The highest insemination dose was obtained from breed D x P in winter (26.0 ± 0.51). Correlation analyses indicated that PLW and D x P boars are the least resistant to higher ambient temperatures, and in summer and autumn this resulted in a reduction in sperm concentration (-0.26 and -0.20, respectively).

D., Knecht; S., & #346; rodo& #324; ; K., Duzi& #324; ski.

2014-01-01

255

Tissue polypeptide antigen immunoreactivity in human semen.  

Science.gov (United States)

A substance with TPA immunoreactivity is present in human seminal plasma. This seminal plasma TPA shows immunological similarity with TPA isolated from a pool of carcinoma tumors. TPA is taken up by live spermatozoa and it cannot be easily washed out. Split-ejaculate studies suggest that TPA originates mainly from the prostate. TPA is not correlated with the spermatozoa count, total number of spermatozoa, percentage of normal spermatozoa, or forward motility. The mean seminal plasma concentration of TPA in morphologically normal ejaculates is 14,531 U/liter. No difference was found in the TPA levels between the normal group and various groups of patients under investigation for involuntary infertility. PMID:6537749

Lizana, J; Eneroth, P; Bygdeman, M

1984-01-01

256

Infecciones de transmisión sexual, calidad del semen e infertilidad / Sexually transmitted diseases, quality of semen, and infertility  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish El propósito de este trabajo es insistir en la asociación que existe entre el incremento de las infecciones de transmisión sexual y la infertilidad masculina. Se muestran numerosas investigaciones realizadas en este campo que demuestran el rol de estas infecciones en la etiología de la infertilidad, [...] y se describen algunas de las principales infecciones en el semen que provocan la declinación de la fertilidad masculina y sus consecuencias en la salud reproductiva de los hombres. Con este trabajo de revisión nos proponemos resaltar la necesidad de incluir en el estudio de la infertilidad masculina el control microbiológico del semen, particularmente útil en los servicios de salud reproductiva, donde el riesgo de prevalencia de infecciones asociadas a la infertilidad provoca una reproducción fallida con consecuencias emocionales y sociales en la pareja Abstract in english Aim of this paper is to insist on association between the sexually transmitted diseases increase and male infertility. We present most researches performed in this field emphasizing the role of these infections in infertility origin, and we describe also some of main semen infections causing decreas [...] e of male fertility, and its consequences on reproductive health of men. Aim of this review paper is to highlight the need of to include in male infertility study, the metabolic control of semen, where risk of infections prevalence associated to infertility provokes a failure reproduction with emotional and social consequences in couple

Bertha Victoria, Rodríguez Pendás; Felipe, Santana Pérez.

2008-12-01

257

Air pollution and decreased semen quality: A comparative study of Chongqing urban and rural areas  

International Nuclear Information System (INIS)

To investigate the association and effects of air pollution level on male semen quality in urban and rural areas, this study examines the outdoor concentrations of particulate matter (PM10), sulfur dioxide (SO2), nitrous dioxide (NO2) and semen quality outcomes for 1346 volunteers in both urban and rural areas in Chongqing, China. We found the urban area has a higher pollution level than the rural area, contrasted with better semen quality in the rural residents, especially for sperm morphology and computer assistant semen analysis (CASA) motility parameters. A multivariate linear regression analysis demonstrates that concentrations of PM10, SO2, and NO2 significantly and negatively are associated with normal sperm morphology percentage (P 10, SO2, and NO2 in urban ambient air may account for worse semen quality in urban males. - Highlights: • We investigate the distributions of PM10, SO2 and NO2 in urban and rural areas in Chongqing, China. • We explore the associations of air pollution and male semen quality. • The concentrations of PM10, SO2, and NO2 are significantly higher in urban areas. • Median values of some semen quality parameters in rural male were higher than urban male. • PM10, SO2, and NO2 were negatively associated with semen quality parameters. - Air pollution is higher in the urban area while there is better semen quality in rural males. Polluted air may thus account for worse semen quality in urban males

258

Obtención y evaluación del semen de capibara Hydrochoerus hydrochaeris / Collection and evaluation of semen from the Capybara Hydrochoerus hydrochaeris  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Objetivo. Determinar las características del semen y la morfometría de los espermatozoides del Capibara (Hydrochoerus hydrochaeris). Materiales y métodos. Se utilizaron 10 machos con peso entre 21-45 kg, los cuales fueron restringidos y anestesiados. El semen se obtuvo mediante electroeyaculación y [...] se determinó el color, volumen, pH, motilidad en masa, motilidad individual, viabilidad, concentración y morfología. Se realizaron además mediciones de la cabeza y la cola de los espermatozoides. Resultados. Se obtuvo semen en el 100% (10/10) de los animales. El mayor número de eyaculaciones (80%; 8/10), se obtuvo con un voltaje máximo de 6V. El color fue blanco, de aspecto lechoso, los valores promedio fueron volumen 135.5±93.56 µl, pH 8.14±0.38, motilidad masal 32.60±13.46%, motilidad individual 34±19.81%, viabilidad 51.3±19.42%, concentración espermática 127±59.01x106 espermatozoides/mL y morfología 51.3±19.42 espermatozoides normales. La longitud de la cabeza fue 5.41±0.7 µm, el ancho de la cabeza 3.77±0.5 µm y área de la cabeza 75.66±20.6 µm². La longitud de la cola fue 27.9±11.3 µm. Conclusiones. La obtención del semen fue satisfactoria mediante electroeyaculación, sin presentar notables diferencias en las características del semen y morfología de los espermatozoides con otros roedores silvestres de menor tamaño, aunque se observó una alta variabilidad de estas características entre los animales muestreados posiblemente por la heterogeneidad de los animales experimentales. Abstract in english Objective. Determine the characteristics of semen and morphometry of the Capybara (Hydrochoerus hydrochaeris) spermatozoid. Materials and methods. 10 males, weighing between 21-45 kg, which were restrained and anesthetized, were used in the study. The semen sample was obtained by electroejaculation [...] and the color, volume, pH, mass motility, individual motility, viability, concentration and morphology were determined. Measurements of the head and tail of spermatozoids were also conducted. Results. Semen was obtained from 100% (10/10) of the animals. The highest number of ejaculations (80%; 8/10) was obtained with a maximum voltage of 6V. The color was white, of a milky appearance, average values were volume 135.5 ± 93.56 µl, pH 8.14 ± 0.38, mass motility 32.60 ± 13.46%, individual motility 34 ± 19.81%, viability 19.42 ± 51.3%, sperm concentration 127 ± 59.01x106 spermatozoids / mL and morphology 51.3 ± 19.42 normal spermatozoids. The head length was 5.41 ± 0.7µm, the width of head 3.77 ± 0.5 and head area 75.66 ± 20.6 µm². The tail length was of 27.9 ± 11.3 µm. Conclusions. Semen collection by electro ejaculation was successful, without presenting significant differences in semen characteristics and spermatozoid morphology with other smaller wild rodents, although there was a high variability of these characteristics observed between the animals sampled, possibly due to the heterogeneity of the experimental animals.

José, Rodríguez P; Miguel, Peña J; Agustín, Góngora O; Ricardo, Murillo P.

2991-29-01

259

Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays  

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Full Text Available Hypo-osmotic swelling test (HOST, eosin-nigrosin staining and normal apical ridge test (NAR were used to determine integrity of plasma membrane and acrosome of undiluted, diluted (cooled to 5oC and frozen-thawed sperm. Semen from seven bulls was used. For diluted and frozen-thawed sperm, three doses were pooled at 37oC. Percentage motility was assessed using a phase contrast microscope. A 50?l each of undiluted, diluted and frozen-thawed semen was mixed with 500?l of 50, 100, 150, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37oC for 1 h. Total number of intact sperm (live of undiluted, diluted and frozen-thawed were assessed before HOST. Percentage motility decreased (P0.05, but varied significantly (P<0.05 within bulls. In conclusion, plasma membrane integrity of undiluted and diluted sperm was compromised during freezing and thawing. However, freezing had no effect on acrosomal integrity.

A. Ijaz

2010-02-01

260

Quantitation of seminal factor IX and factor IXa in fertile, nonfertile, and vasectomy subjects: a step closer toward identifying a functional clotting system in human semen.  

Science.gov (United States)

Coagulation factor (F) IX is a zymogen of the plasma serine proteases, one that plays an essential role in the regulation of normal blood coagulation. Congenital defects of FIX synthesis or function cause hemophilia B (originally called hemophilia C). Factor IX is activated by Tissue Factor (TF):FVII/FVIIa complex and FXIa. Subsequent to its activation, FIXa combines with FVIIIa on the platelet surface and activates FX to FXa. Human semen forms a semi-solid gelatinous coagulum, which then liquefies within 5-20 minutes in vitro. In spite of evidence demonstrating the importance of the seminal coagulation and liquefaction process in terms of global fertility and despite the fact that the seminal coagulum is composed of fibrin-like material, it has always been addressed from the perspective of High Molecular Weight Seminal Vesicle (HMW-SV) proteins (Semenogelin I and II) and their cleavage by prostate-specific antigen rather than the conventional hemostatic factors. In this study and as part of our continuing investigation of human seminal clotting factors, we report here on seminal FIX and FIXa in normal, subfertile, and vasectomized subjects. Factors IX and FIXa were studied in a total of 119 semen specimens obtained from subfertile (n=18), normally fertile (n=34), and fertile sperm donors (n=27) and vasectomy subjects (n=40). Seminal FIX and FIXa levels were also measured in a group defined by normality in several parameters derived from the World Health Organization fertility criteria and termed "pooled normal semen parameters." Both FIX and FIXa were quantifiable in human semen. There was a wide individual variation in FIX and FIXa levels within groups. Despite the group size, statistically significant associations with fertility-related parameters were infrequent. There is a positive correlation between FIX and its activation product, FIXa (n=36; r=0.51; P <.05). Factor IXa elevation in the high sperm-clump group was significant (P <.05), and days of abstention correlated with FIXa levels (n=63; r=0.3; P <.05). The key finding of the present study is that both FIX and FIXa are present in concentrations that are not dissimilar to plasma levels and that are apparently functional, as the activated form is also present. This fact, taken with other reports of coagulation factors in semen, raises the likelihood that a functional set of hemostatic coagulation proteins exists in semen, potentially to interact with the HMW-SV proteins and the prostate-specific antigen system. PMID:15611579

Lwaleed, Bashir A; Greenfield, Robert S; Hicks, James; Birch, Brian R; Cooper, Alan J

2005-01-01

261

Evaluación del sistema antioxidante en el semen normal / Evaluation of antioxidant system in normal semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO Mexico | Language: Spanish Abstract in spanish Antecedentes. Las especies reactivas del oxígeno (ERO), tienen la capacidad de alterar reversible o irreversiblemente la función celular. Se ha propuesto que las ERO modifican la bioquímica y la fisiología del espermatozoide. Por otro lado, los mecanismos antioxidativos pudieran proteger a los esper [...] matozoides del daño producido por las ERO. Objetivo. Determinar los valores normales para el superóxido dismutasa (SOD), glutatión peroxidasa (GPx), malondialdehído (MDA) y óxido nítrico (NOx) en el líquido seminal y espermatozoides de humanos sanos. Procedimientos. Se estudiaron 45 muestras de semen de sujetos aparentemente sanos. Las muestras se obtuvieron por masturbación y se colectaron en tubos estériles. Una vez centrifugadas, se fraccionaron en alícuotas para medir la concentración de SOD, GPx, MDA y NOx. El análisis de las muestras se realizó conforme a métodos bioquímicos ampliamente aceptados. Resultados. Las concentraciones de SOD y MDA en el líquido seminal como en los espermatozoides fueron similares (SOD 0.43 ± 0.09 en semen y 0.45 ± .07 U/mg prot. en espermatozoides, y MDA 0.33 ± .07 y 0.37 ± 0.10 nmoles/mg prot. en líquido seminal y espermatozoides, respectivamente. Con respecto a la GPx, está aumentada casi 13 veces más en los espermatozoides (2547.77 ± 48.59 U/mg prot.) que en el líquido seminal (197.54 ± 25.21 U/mg prot.), el NOx también se incrementa ligeramente en los espermatozoides (4.45 ± 0.43 µmol) cuando se compara con el líquido seminal (3.91 ± 0.16 µmol). Conclusiones. La medición de los antioxidantes y oxidantes pudieran servir para evaluar la infertilidad humana en aquellos casos donde los resultados de la espermatobioscopia aparezcan como normales. Abstract in english Background. Reactive oxygen species (ROS) formation have the ability to alter reversibly or irreversibly the cellular function in humans. It has been proposed that the ROS alters the biochemistry and the physiology of the sperm. On the other hand, the antioxidative mechanisms could protect the sperm [...] s from the damage produced by free radicals. Aim. To determine the normal values for superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and nitric oxide (NOx) in the seminal liquid of healthy humans. Procedures. Semen samples from 45 healthy men (22 to 47 years of age) were studied. The samples were obtained by masturbation and were collected in conical sterile tubes. Once centrifuged at 4 °C they were divided in aliquots to measure the concentration of SOD, GPx, MDA, and NOx. The analysis of the samples was realized in conformity with biochemical widely accepted methods. Results. The concentrations of SOD and MDA both in the seminal liquid and in the spermatozoids were similar, SOD 0.43 ± 0.09 U/mg prot. in the seminal liquid and 0.45 ± 0.07 U/ mg prot. in spermatozoids, and MDA 0.33 ± 0.07 nmoles/mg prot. and 0.37 ± 0.10 nmoles/mg prot. in the seminal liquid and spermatozoids respectively. With regard to GPx it increased almost 13 times more in the spermatozoids (2547.77 ± 48.59 U/mg prot.) than in the seminal liquid (197.54 ± 25.21 U/mg prot.). The NOx also increased lightly in the spermatozoids (4.45 ± 0.43 \\imol) when compared with the seminal liquid (3.91 ± 0.16 \\imol). Conclusions. The measurement of the antioxidative and oxidative agents could serve to evaluate human infertility in those cases where the result of the spematobioscopy appears normal.

Juan M., Gallardo.

2007-02-01

262

Influence of Deficiency or Supplementary Selenium and a- Tochopherol (Vitamin E) In The Diet of Pubertal Male Zaraibi Goats on Fertility, Semen Quality and Testicular Traits  

International Nuclear Information System (INIS)

Twenty pubertal male Zaraibi goats (bucks) were randomly divided into four equal groups; fed deficient Se or vit. E, adequate Se, adequate vit. E and adequate Se + vit. E diets for 3 months to study the influence of deficient or adequate selenium (Se) and vitamin E (vit. E) in the diet of pubertal male Zaraibi goats on fertility, semen quantity and quality and some testicular traits. The results showed that the best values of semen quantity (the ejaculate volume, sperm concentration and total sperm output per ejaculate) and semen quality (percentage of progressive motility, percentage of live sperm, number of motile sperm per ejaculate, percentage of dead, abnormal spermatozoa and acrosomal abnormality) were observed in bucks fed diet supplemented with adequate Se combined with adequate vit. E. The lowest values of semen quantity and semen quality were observed in bucks suffering from deficiency of Se and/or vit. E in their diets. Testosterone level in seminal plasma was significantly higher in bucks fed adequate Se and/or vit. E than those fed diet deficient in Se and vit. E. Testosterone level was significantly higher in bucks fed diet adequate in Se + vit. E than those fed diet adequate with Se or vit. E alone. Se and vit. E deficiency in the diets was accompanied by a significant decrease in testosterone, T4 and T3 levels in seminal plasma. Selenium or vit. E each one alone supplementation led to increases of these hormones. T4 and T3 levels were significantly higher in bucks fed adequate Se or adequate Se + vit. E than in bucks fed diet with adequate vitamin E alone. Adequate Se alone and adequate Se + vit. E diets were accompanied by significant increases in Se in seminal plasma. Adequate vit. E and adequate Se + vit. E diets were accompanied by significant increase in vit. E level in the seminal plasma. It is clear that there was synergism between Se and vit. E in the biological role of Se, since the level of Se in bucks fed diet containing adequate Se + vit. E was higher than the level of Se in group fed Se alone. The highest values of scrotal circumference and scrotum length were observed in bucks fed adequate Se + vit. E and the lowest testicular traits and fertility were observed in bucks fed diet deficient with Se and vit. E.

263

Clinical and biochemical correlates of successful semen collection for cryopreservation from 12-18-year-old patients: a single-center study of 86 adolescents  

DEFF Research Database (Denmark)

Cryopreservation of semen should be offered to adults before gonadotoxic treatment. However, the experience with semen collection in adolescents is still limited. The objective of this study was to evaluate potential correlates of successful semen sampling in adolescents.

Hagenäs, Isabella; JØrgensen, Niels

2010-01-01

264

Functional characterisation of semen in honeybee queen (A.m.ligustica S. spermatheca and efficiency of the diluted semen technique in instrumental insemination  

Directory of Open Access Journals (Sweden)

Full Text Available Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica queen spermatheca werestudied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05 between the 12th and 24th month.The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminatedtwice with a Hyes solution/semen mixture (1:1 stored as many spermatozoa in their spermatheca as those inseminatedonce with the classic technique. Queen replacement, oviposition and other functional characteristics were similarto those observed in the classic insemination procedure.

Andrea Galli

2010-01-01

265

Studies on Freezing RAM Semen in Absence of Glycerol.  

Science.gov (United States)

Glycerol is widely used as a major cryoprotective agent for freezing spermatozoa of almost all species. However, it reduces fertility of sheep inseminated cervically compared with intrauterine insemination. Studies were conducted to develop a method and procedure for freezing ram semen in the absence of glycerol. Post -thaw survival of ram spermatozoa frozen in the absence of glycerol was affected by time and temperature after collection and before dilution and time after dilution and before freezing. Increase in time at 5^ circC before or after dilution and before freezing increased both post-thaw motility and number of cells passing through Sephadex filter. A cold dilution method was developed. Slow cooling of fresh ram semen and diluting at 5^circ C 2-3 hr. after collection, then freezing 1 hr. after dilution improved both post-thaw motility and number of cells passing through Sephadex filter compared with immediate dilution at 30-37^circC after collection and freezing 3-4 hr. later (P ram semen in the absence of glycerol. An increase in post-thaw motility was obtained when semen was extended in TES titrated with Tris to pH 7.0 (TEST) and osmotic pressure of 375-400 mOsm/kg, containing 25-30% (v/v) egg yolk and 10% (v/v) maltose. A special device (boat) for freezing was constructed to insure the same height of the sample above LN _2 and thus the same freezing rate from freeze to freeze. Freezing of semen in 0.25cc straws at 5-10 cm above LN_2 (73.8 to 49.5 ^circC/min) yielded higher post-thaw motility than the rates resulted from freezing at 15 cm above LN_2 or 1 cm above LN _2. Faster Thawing in 37^ circC water for 30 sec. (7.8^ circC/sec.) increased post-thaw motility compared with slower thawing in 5 or 20^circ C water (P ram semen frozen without glycerol and 17.1% in a second trial. One injection (IM) of 15 mg PGF_{2alpha}/ewe for estrus synchronization during breeding season resulted in higher heat response and lambing rate than two injections given 10 days apart.

Abdelnaby, Abdelhady Abdelhakeam

1988-12-01

266

Quantification of damage at different stages of cryopreservation of endangered North American bison (Bison bison) semen and the effects of extender and freeze rate on post-thaw sperm quality.  

Science.gov (United States)

Semen cryopreservation is an important technique for the banking of animal germplasm from endangered species and exploitation of genetically superior sires through artificial insemination. Being a member of bovidae family, bison semen has poor freezing ability as compared to dairy and beef bulls' semen. This study was designed to quantify the damage to bison sperm at different stages of cryopreservation, and to determine the effects of extender (commercial Triladyl(®) vs. custom made tris-citric acid [TCA]) and freeze rate (-10, -25 and -40°C/min) on post-thaw quality of bison semen. Semen was collected from five bison bulls (three woods and two plains) via electroejaculation. In Experiment 1, semen was diluted in Triladyl® extender and frozen with freeze rate -10°C/min. Sperm motility characteristics were recorded in fresh, diluted, cooled (4°C) and freeze-thawed semen using computer-assisted sperm analyzer (CASA). In Experiment 2, semen was diluted in Triladyl® or TCA extender, and frozen with three different freeze rates, i.e. -10, -25 or -40°C/min. Thawing was performed at 37°C for 60s. Post-thaw sperm motility characteristics were assessed using CASA, and sperm structural characteristics (plasma membrane, mitochondrial membrane potential and acrosomes) were evaluated using flow cytometer, at 0 and 3h while incubating semen at 37°C. In Experiment 1, total and progressive motilities did not differ among pre-freeze stages of cryopreservation (P>0.05). However, sperm total and progressive motilities declined (P<0.001) in freeze-thawed semen by 35% and 42%, respectively, compared to after cooling (pre-freeze) semen. In Experiment 2, Triladyl®, as compared to TCA, yielded greater (P<0.05) post-thaw sperm total motility (41% compared to 36%) and progressive motility (34% compared to 29%) at 0h, respectively. The percent change in post-thaw sperm total and progressive motilities, VAP, VCL, VSL, IPM-high ??m and IPM-IACR during 3h incubation at 37°C, was less (P<0.05) in TCA than in Triladyl®. There was an effect of freeze rate on post-thaw sperm average path velocity at 0h, and total motility, progressive motility, VCL, IPM and IPM-IACR at 3h were the greatest (P<0.05) when bison semen was frozen at -40°C/min. Likewise, the percent change in post-thaw sperm total and progressive motilities, during 3h incubation at 37°C, was less (P<0.05) in bison semen frozen at -40°C/min. All post-thaw bison sperm characteristics decreased (P<0.05) from 0h to 3h, during incubation at 37°C. In conclusion, the maximum damage to bison sperm occurred during freeze-thaw processes. Post-thaw total and progressive motilities of bison sperm were greater in Triladyl® at 0h whereas sperm survival was greater in TCA extender during 3h post-thaw incubation. Bison sperm had greater survival (P<0.05) when frozen at -40°C/min freeze rate. PMID:22240453

Hussain, S A; Lessard, C; Anzar, M

2011-12-01

267

Efecto de dos dilutores sobre la motilidad e integridad de la membrana espermática en semen congelado de ovinos / Effects of two semen extenders on motility and integrity of sperm membrane in ovine frozen semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish El presente estudio tuvo como objetivo evaluar el efecto de dos dilutores, Tris- Fructosa-Yema de huevo (Tris) y Citrato-Glucosa-Yema de huevo (citrato), sobre la motilidad espermática e integridad de la membrana espermática (HOST) en semen congelado de ovinos bajo la forma de pellets. La investigac [...] ión se llevó a cabo en el Banco de Semen de la Universidad Nacional Agraria La Molina, Lima, empleándose 4 carneros (2 Blackbelly y 2 Assaf) de 3.5 a 4 años de edad. Se empleó el análisis de covariancia para analizar Motilidad Individual Progresiva (MIP), y bloques completamente randomizados para medir el efecto de los dilutores sobre la integridad de la membrana espermática. Para el congelamiento del semen se utilizó hielo seco y el descongelamiento se realizó a 38 ºC en tubos de ensayo. En ovinos Assaf, la MIP del semen descongelado fue de 63.77 y 61.11% utilizando Tris y citrato, respectivamente, encontrándose diferencias significativas (p Abstract in english The objective of the study was to evaluate the effects of two semen extenders: Tris- Fructose-egg yolk (Tris) and Citrate-Glucose-egg yolk (citrate) on motility and sperm membrane integrity (HOST) in ovine frozen semen in pellets. The study was carried out at the Semen Bank of the Agrarian Universit [...] y La Molina, in Lima, Peru, using 4 rams (2 Assaf and 2 Blackbelly) of 3.5 to 4 years old. A covariance analysis was used to evaluate the effect of the treatment and breed on Individual Progressive Motility (IPM), and randomized block design to evaluate the effect of extenders on sperm membrane integrity. Semen was frozen of dry ice and thawing was done in test tubes at 38 °C. In the Assaf breed, IPM of thawed semen was 63.77 and 61.11% when using Tris and citrate respectively, showing statistical difference (p

Próspero, Cabrera V; Javier, Orellana Ch; César, Pantoja A.

2010-07-01

268

Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study  

OpenAIRE

The Malayan gaur (Bos gaurus hubbacki) or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN). The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM) and electroejaculation (EEJ) technique was applied in semen collection of the Malayan gaur. The semen w...

Khairiah, M. S.; Zawawi, I.; Wahid, H.; Hajarian, H.; Fahrul, F. J.; Hafiz, M. D.; Hafiz, M. M.; Ann, Z. F.; Iswadi, M. I.; Mazni, O. A.

2012-01-01

269

Characterization and differentiation of normal and abnormal semen samples using micro-Raman spectroscopy  

Science.gov (United States)

Growth in the percentage of male infertility has caused extensive concerns. The fast and reliable method is urgently required for diagnosis of semen samples. In our study, micro-Raman spectroscopy was employed to characterize and differentiate the normal and abnormal semen samples based on the differences of their specific Raman spectra which originated from biochemical components. Our preliminary results demonstrate that micro-Raman spectroscopy combined with multivariate analysis methods has the potential of being used to detect and differentiate semen samples.

Huang, Zufang; Chen, Xiwen; Chen, Jinhua; Li, Yongzeng; Lei, Jinping; Chen, Rong

2012-12-01

270

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen  

OpenAIRE

Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) ext...

Kaeoket, Kampon; Chanapiwat, Panida; Tummaruk, Padet; Techakumphu, Mongkol

2010-01-01

271

Peptides Released by Physiological Cleavage of Semen Coagulum Proteins Form Amyloids that Enhance HIV Infection  

OpenAIRE

Semen serves as a vehicle for HIV and promotes sexual transmission of the virus, which accounts for the majority of new HIV cases. The major component of semen is the coagulum, a viscous structure composed predominantly of spermatozoa and semenogelin proteins. Due to the activity of the semen protease PSA, the coagulum is liquefied and semenogelins are cleaved into smaller fragments. Here, we report that a subset of these semenogelin fragments form amyloid fibrils that greatly enhance HIV inf...

Roan, Nadia R.; Mu?ller, Janis A.; Liu, Haichuan; Chu, Simon; Arnold, Franziska; Stu?rzel, Christina; Walther, Paul; Dong, Ming; Witkowska, H. Ewa; Kirchhoff, Frank; Mu?nch, Jan; Greene, Warner C.

2011-01-01

272

Liquefaction of Semen Generates and Later Degrades a Conserved Semenogelin Peptide That Enhances HIV Infection  

OpenAIRE

Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exhibits maximal HIV-enhancing activity that gradually declines upon further incubation. The decline in HIV-enhancing activity parallels the degradation of peptide fragments derived from the ...

Roan, Nadia R.; Liu, Haichuan; Usmani, Shariq M.; Neidleman, Jason; Mu?ller, Janis A.; Avila-herrera, Aram; Gawanbacht, Ali; Zirafi, Onofrio; Chu, Simon; Dong, Ming; Kumar, Senthil T.; Smith, James F.; Pollard, Katherine S.; Fa?ndrich, Marcus; Kirchhoff, Frank

2014-01-01

273

A novel method for semen collection and artificial insemination in large parrots (Psittaciformes)  

OpenAIRE

The paper described a novel technique for semen collection in large psittacines (patent pending), a procedure which was not routinely possible before. For the first time, a large set of semen samples is now available for analysis as well as for artificial insemination. Semen samples of more than 100 psittacine taxa were collected and analysed; data demonstrate large differences in the spermatological parameters between families, indicating an ecological relationship with breeding behaviour (p...

Michael Lierz; Matthias Reinschmidt; Heiner Müller; Michael Wink; Daniel Neumann

2013-01-01

274

Semen Parameters in Fertile US Men: The Study for Future Families  

OpenAIRE

Establishing reference norms for semen parameters in fertile men is important for accurate assessment, counseling and treatment of men with male factor infertility. Identifying temporal or geographic variability in semen quality also requires accurate measurement of semen parameters in well-characterized, defined populations of men. The Study for Future Families (SFF) recruited men who were partners of pregnant women attending prenatal clinics in Los Angeles CA, Minneapolis MN, Columbia MO, N...

Redmon, J. Bruce; Thomas, William; Ma, Wenjun; Drobnis, Erma Z.; Sparks, Amy; Wang, Christina; Brazil, Charlene; Overstreet, James W.; Liu, Fan; Swan, Shanna H.

2013-01-01

275

The Viability of Local Ram Semen in Tris Buffer With Three Different Egg Yolks  

OpenAIRE

Egg yolk consisted of lecithin and phospholipids are one of the most commonly used components that will protect spermatozoa against cold shock during cooling and freezing. The aim of this study was to evaluate the effect of different hen egg yolk on Tris extender on the freezability of local ram semen. Semen from six sexually matured local rams was collected weekly using artificial vagina. Collected semen was evaluated macroscopically and microscopically and extended using tris extender con...

Wmm, Nalley; Ri, Arifiantini

2011-01-01

276

Semen quality in welders before and after three weeks of non-exposure.  

OpenAIRE

In a cross sectional field study concerning the male reproductive system in metalworkers, the major findings were a moderate deterioration of semen quality in mild steel welders and less reliable changes in semen quality in low exposed stainless steel welders. In the present study, a longitudinal design was adopted to deal with methodological drawbacks inherent in the cross sectional approach. The study relies on the assumption that the effect of welding is causal and reversible. The semen qu...

Bonde, J. P.

1990-01-01

277

Comparison of different extenders on the quality characteristics of turkey semen during storage  

OpenAIRE

Semen of the domestic turkey cannot be stored longer than 6 h without a loss of fertilizing capability. The improvement of long-term liquid storage procedures of semen is important since the commercial production of turkey relies almost entirely on artificial insemination. Therefore, studies improving storage regimens would allow longer storage and consequently hen fertility (Iaffaldano and Meluzzi, 2003). Since the search for an optimal extender composition for semen storage is still in prog...

Meluzzi, A.; Centoducati, G.; Manchisi, A.; Rosato, M. P.; Iaffaldano, N.

2010-01-01

278

Decline of semen quality and increase of leukocytes with cigarette smoking in infertile men  

OpenAIRE

Background: Previous researches about the effect of smoking on semen quality are contradictory, and the mechanism behind the harmful effect of smoking on semen quality still remains unclear until today. Objective: The objectives of this study are evaluation of the relationship between smoking and fertility, investigation of the effects of cigarette smoking on sperm parameters and detection of presence of leukocytes within the semen of idiopathic infertile men from Northeastern China. Material...

Zhi Hong Zhang; Hai Bo Zhu; Lei Lei Li; ang Yu; Hong Guo Zhang; Rui Zhi Liu

2013-01-01

279

Cryopreservation of rooster semen in thirteen and sixteen percent glycerol.  

Science.gov (United States)

Semen from Barred Plymouth Rock roosters was cryopreserved with glycerol concentrations of 13 and 16% in a microprocessor-controlled freezer. Thawing and deglycerolation were facilitated by the use of an improved microprocessor-controlled thawing device and high speed dialyzer. Deglycerolated semen (100 mu L; 192 and 154 million sperm, respectively, for the 13 and 16% glycerol concentration) was inseminated into Single Comb white Leghorn hens. Three inseminations were done at 4-d intervals. Eggs were collected for 10 d starting 1 d after the first insemination, and incubated for 4th d. Fertility was determined by candling after the 4th d. Fertility measurements of 62.4 and 65% were obtained from the sperm frozen in 13 and 16% glycerol concentrations, respectively, for the 10-d period. PMID:8833379

Gill, S P; Buss, E G; Mallis, R J

1996-02-01

280

Effect of L-(+)-Ergothioneine (EGT) on Freezability of Ram Semen  

OpenAIRE

The aim of this study was to investigate freezability of ram semen extended with different L-(+)-Ergothioneine (EGT) doses. For this aim, semen from four ram were collected with artificial vagina (44°C) and then pooled. Pooled semen was divided five aliquots and extended with skim milk based extender containing 0 mmol/L (EGT0: Control), 1 mmol/L (EGT1), 2 mmol/L (EGT2), 5 mmol/L (EGT5) and 10 mmol/L (EGT10) EGT, respectively. After equilibration (+5°C/2 h), the extended aliquots of semen in...

Ari, U. C.; Kulaksiz, R.; Ozturkler, Y.; Yildiz, S.; Lehimcioglu, N. C.

2012-01-01

281

Flow cytometric assessment of fresh and frozen-thawed Canada goose (Branta canadensis) semen.  

Science.gov (United States)

The present study was conducted to investigate spermatozoal membrane integrity, acrosome integrity, mitochondrial activity, and chromatin structure in fresh and frozen-thawed Canada goose (Branta canadensis) semen with the use of the flow cytometry. The experiment was carried out on ten, 2-year-old, Canada goose ganders. The semen was collected twice a week, by a dorso-abdominal massage method, then pooled and subjected to cryopreservation in straws, in a programmable freezing unit with the use of dimethyloformamide (DMF) as a cryoprotectant. Frozen samples were thawed in a water bath at 60 °C. The freezing procedure was performed ten times. For the cytometric analysis the fresh and the frozen-thawed semen was extended with EK extender to a final concentration of 50 million spermatozoa per mL. Sperm membrane integrity was assessed with SYBR-14 and propidium iodide (PI), acrosomal damage was evaluated with the use of PNA-Alexa Fluor®488 conjugate, mitochondrial activity was estimated with Rhodamine 123 (R123), and spermatozoal DNA integrity was measured by the sperm chromatin structure assay (SCSA). The cryopreservation of Canada goose semen significantly decreased the percentage of live cells, from 76.3 to 50.4% (P semen than in the fresh semen (P semen. In conclusion, the present study indicates that even the fresh Branta canadensis semen might have poor quality, the cryopreservation of its semen did not provoke spermatozoal DNA defragmentation and half of the spermatozoa retained intact acrosomes and active mitochondria after freezing-thawing. PMID:21664666

Partyka, Agnieszka; ?ukaszewicz, Ewa; Ni?a?ski, Wojciech

2011-09-15

282

Factors associated with the determination of antibiotic activity in bovine semen.  

OpenAIRE

Rosaramicin, an agent shown to be effective in vitro against ureaplasma of bovine origin was tested as an additive to bovine semen extender. Although some reduction in semen quality occurred it was still deemed satisfactory for use. In a test involving 41 cows inseminated once at estrus with rosaramicin-treated semen (162 mcg/mL) the nonreturn rate was 24% compared to a calculated average for this semen of 63% (n = 3310). The effect of centrifugation, time and temperature was examined in vitr...

Truscott, R. B.

1983-01-01

283

Comparison of different extenders on the quality characteristics of turkey semen during storage  

Directory of Open Access Journals (Sweden)

Full Text Available Semen of the domestic turkey cannot be stored longer than 6 h without a loss of fertilizing capability. The improvement of long-term liquid storage procedures of semen is important since the commercial production of turkey relies almost entirely on artificial insemination. Therefore, studies improving storage regimens would allow longer storage and consequently hen fertility (Iaffaldano and Meluzzi, 2003. Since the search for an optimal extender composition for semen storage is still in progress, the aim of this paper has been to study the effects of different extenders on the quality of turkey semen during the storage for 48 h at 5°C.

A. Meluzzi

2010-01-01

284

Effect of L-carnitine supplementation on drake semen quality  

Scientific Electronic Library Online (English)

Full Text Available This study was conducted to determine the effect on semen quality traits of supplementing the diets of Iraqi drakes with L-carnitine. Forty eight male Iraqi ducks, 30 weeks old, were randomly allocated to four treatments with 12 drakes per treatment group, replicated three times, with four drakes pe [...] r replicate. The treatment groups consisted of birds fed a diet free of L-carnitine (T1, control group); birds fed a diet containing 50 mg L-carnitine/kg diet (T2); birds fed a diet containing 100 mg L-carnitine/kg diet (T3); and birds fed a diet containing 150 mg L-carnitine/kg diet. The drakes were fed the experimental diets only during the experimental period, which lasted three months. The semen quality traits that were investigated were ejaculate volume, mass and individual motility of spermatozoa, spermatocrit, spermatozoa concentration, percentages of dead and abnormal spermatozoa and acrosomal abnormalities. Supplementing the diet of drakes with L-carnitine at the levels of 50 - 150 mg/kg diet significantly increased ejaculate volume, spermatocrit, mass and individual motility of spermatozoa, and concentration of spermatozoa, while percentages of dead and abnormal spermatozoa and acrosomal abnormalities were decreased. However, T4 (150 mg L-carnitine/kg diet) recorded the best results in relation to all semen quality traits included in this study. Dietary supplementation with L-carnitine improved the semen quality of local drakes; therefore L-carnitine can be used as an efficient feed additive to improve the reproductive performance of male ducks.

H.J., Al-Daraji; A.O., Tahir.

2014-01-01

285

Preservation of mithun (Bos frontalis) semen at refrigeration temperature.  

Science.gov (United States)

The objective of the present study was to investigate the possibility of preserving mithun (Bos frontalis) spermatozoa at refrigeration temperature using tris-egg yolk diluent. Semen samples were collected from four adult mithun bulls through rectal massage method. Good quality semen samples (n=30) were preserved at 4 degrees C using tris-egg yolk diluent for 72 h. Progressive motility, live spermatozoa count and morphological abnormalities were evaluated every 12 h until 72 h of preservation. The colour, consistency and mass activity of fresh semen samples were found to be creamy white, medium and 3+ to 4+ (5+ scale), respectively. The average (mean+/-S.E.) volume (ml), pH and spermatozoa concentration (10(6) ml(-1)) of fresh semen samples were found to be 0.6+/-0.01, 6.8+/-0.03 and 425+/-48, respectively. Progressive motility and live spermatozoa count were found to be less than 30% (P<0.01) after 48 h of storage. Head (P<0.05), midpiece (P<0.05), tail (P<0.01) and total (P<0.01) abnormalities were found to be increased significantly over the time of storage. It was observed that progressive motility and live spermatozoa count remained above 30% and 40%, respectively, until 36 h of storage. Simultaneously the percentage of morphologically abnormal spermatozoa was found to be significantly low until 36 h of storage. The results indicate that it is possible to preserve mithun spermatozoa at refrigeration temperature in tris-egg yolk diluent, which can be further used for artificial insemination within 36 h of storage. PMID:17095169

Karunakaran, M; Dhali, A; Mech, A; Khate, K; Rajkhowa, C; Mishra, D P

2007-10-01

286

Semen decontamination for the elimination of seminal pathogens  

OpenAIRE

The presence of pathogens in semen can compromise the outcome of assisted reproductive treatment, together with the possibility of the female partner or offspring becoming infected. This is cause for concern, especially in South Africa with a high prevalence of HIV-1. Most of these infected individuals are in their reproductive years with the desire to have their own genetically related children. Therefore, assisted reproductive treatment with effective risk reduction procedures, such as seme...

Fourie, Jozef Markus

2013-01-01

287

Semen collection and preservation in African catfish, Clarias gariepinus  

OpenAIRE

Stock improvement using quantitative and molecular genetics is an essential part of nowadays production of farm animals and fish. To achieve this in aquaculture, germplasm of both parental sexes should be obtained in a life-saving manner. In captivity, male African catfish, Clariasgariepinus , do not release semen under abdominal massage and have to be sacrificed to obtain sperm from the macerated testes. Of course, this is regarded as a major constrains by the catfish farming sector. Against...

Viveiros, A. T. M.

2002-01-01

288

Seasonality and freezability vs routine parameters in stallion semen  

OpenAIRE

The fertilizing ability of stallion semen was analyzed using fresh and frozen samples, obtained before (June-July) or during (October-November) the breeding season. Thirty ejaculates obtained from 4 stallions, were used. The analysis comprises routine seminogram; ATP concentration (Comhaire et al., 1983); subjective and objective motility and sperm velocity (Makler, 1980). Freezing was done following the technique of Martin et al. (1979). Sperm velocity, ATP ...

Rodri?guez, H.; Bustos-obrego?n, E.

1996-01-01

289

Semen quality in welders exposed to radiant heat.  

OpenAIRE

Several studies suggest that welding is detrimental to the male reproductive system. Welding fume and radiant heat are of interest as possible causal factors. This study investigates semen quality and sex hormone concentrations among 17 manual metal arc alloyed steel welders with a moderate exposure to radiant heat (globe temperature ranging from 31.1 degrees to 44.8 degrees C), but without substantial exposure to welding fume toxicants. During exposure to heat the skin temperature in the gro...

Raymond, L. W.

1992-01-01

290

The importance of semen analysis in the context of azoospermia  

OpenAIRE

Azoospermia is a descriptive term referring to ejaculates that lack spermatozoa without implying a specific underlying cause. The traditional definition of azoospermia is ambiguous, which has ramifications on the diagnostic criteria. This issue is further compounded by the apparent overlap between the definitions of oligospermia and azoospermia. The reliable diagnosis of the absence of spermatozoa in a semen sample is an important criterion not only for diagnosing male infertility but also fo...

Nabil Aziz

2013-01-01

291

Sperm banking for male cancer patients: social and semen profiles  

Directory of Open Access Journals (Sweden)

Full Text Available PURPOSE: Report the characteristics of cryopreserved semen from a cohort of male cancer patients, attitudes towards cryopreservation and outcomes of semen samples based on a 12-year cryopreservation program. MATERIAL AND METHODS: Data from 98 male cancer patients whose sperm samples were banked were evaluated. Demographic parameters, semen characteristics, destination of sperm banked samples and questionnaires answered by the patients regarding cryopreservation time were evaluated. RESULTS: The cancer diagnoses were testicle (56.1%, prostate (15.3%, Hodgkin’s lymphomas (9.2%, non-Hodgkin’s lymphomas (7.1%, leukemia (3.1% and other malignancies (9.2%. The patients with testicular cancer presented lower sperm concentration (p < 0.001; however, there were no differences with the percentage of normozoospermic patients among cancer type groups (p = 0.185. A shorter time between cancer diagnosis and sperm banking was observed for testicular and prostate cancer patients (p < 0.001. Most of the patients (89.5% favored sperm banking as a fertility preservation method. CONCLUSIONS: Although less than 20% of banked sperm samples were disposed of, the majority of patients related sperm banking with safe for fertility preservation. Our results show that all male cancer patients of reproductive age facing cancer treatment could be offered sperm banking.

Tatiana C.S. Bonetti

2009-04-01

292

Prospective surveillance of semen quality in the workplace  

Energy Technology Data Exchange (ETDEWEB)

We performed a prospective surveillance of semen quality among workers in the plant where 1,2-dibromo-3-chloropropane was first recognized as an occupational cause of impaired semen quality and of infertility. All male employees of the Agricultural Chemical Division were required to participate. Ninety-seven workers (92% participation) provided 258 semen samples over the 4 years of the program. Most samples were analyzed at the plant with a mini-laboratory designed for the study. Motility and shape measures were made objectively. Sixty-six subjects (68%) were non-azoospermic. Generalized multiple regression showed no significant predictors for any response, with the exception of the motility measures, which were reduced with longer times between ejaculation and assay. Between- and within-person standard deviations and correlations were calculated. Comparison of this population with fertile artificial insemination donors (16 men, 498 ejaculates) revealed generally higher ejaculate-to-ejaculate standard deviations in the worker samples. This is probably due to less well controlled conditions of sperm collection in the workplace setting. For cross-sectional studies, one ejaculate per worker is recommended as sufficient; for estimating an individual worker's mean, even three ejaculates may not provide enough precision.

Schenker, M.B.; Samuels, S.J.; Perkins, C.; Lewis, E.L.; Katz, D.F.; Overstreet, J.W.

1988-04-01

293

Biochemical properties of microbial load in frozen semen of cattle  

Directory of Open Access Journals (Sweden)

Full Text Available A total 20 French mini straws (0.25ml of frozen semen were randomly collected from one of frozen semen bank for evaluation of microbial load using the standard plate count (SPC method using nutrient agar plate. These plates were incubated at 37oC for 24 and 48 hrs and examined for growth. The average colony count was calculated and bacteria were also identified as Gram positive and Gram negative. A total of 10 biochemical tests were performed to characterize the isolates. Antibiotic sensitivity test was also performed to test the sensitivity against Ampicillin, Erythromycin, Gentamycin, Spectinomycin and Tetracycline. The results indicate that 5 samples out of 20 were found positive for various bacterial isolates and fungi. Both the Gram positive and Gram negative bacteria were found in these samples. One isolate from sample No. CB-594 (white colony was found positive for all 9 biochemical tests. All the bacterial isolates exhibited variable pattern against 5 antibiotics used in the study. The article describes detailed investigation of microbial load in frozen semen of cattle bulls.

Hemaxi V Patel

2011-06-01

294

Semen quality and sex hormones with reference to metal welding  

DEFF Research Database (Denmark)

Welding may involve hazards to the male reproductive system, but previous studies of semen quality have produced inconsistent results. We studied the effects of welding on markers of semen quality in a Danish nationwide sample of 430 first-time pregnancy planners without earlier reproductive experience. Couples were recruited among members of the union of metal workers and three other trade unions and were followed from termination of birth control until pregnancy for a maximum of six menstrual cycles. The males provided semen samples in each cycle. Median sperm density for welders was 56 x 10(6)/mL (52.5 x 10(6)/mL and 50.0 x 10(6)/mL in two reference groups). No statistically significant differences attributable to welding were found in proportions of morphologically normal sperm, sperm motility assessed by computer-aided sperm analysis, or sex hormones (testosterone, follicle-stimulating hormone, and luteinizing hormone). These negative findings may not apply to populations with high-level exposure to welding fume or to welders exposed to other putative hazards, e.g., heat.

Hjollund, N H; Bonde, J P

1998-01-01

295

Decreases in Human Semen Quality with Age Among Healthy Men  

Energy Technology Data Exchange (ETDEWEB)

The objective of this report is to characterize the associations between age and semen quality among healthy active men after controlling for identified covariates. Ninety-seven healthy, nonsmoking men between 22 and 80 years without known fertility problems who worked for or retired from a large research laboratory. There was a gradual decrease in all semen parameters from 22-80 years of age. After adjusting for covariates, volume decreased 0.03 ml per year (p = 0.001); sperm concentration decreased 2.5% per year (p = 0.005); total count decreased 3.6% per year of age (p < 0.001); motility decreased 0.7% per year (P < 0.001); progressive motility decreased 3.1% per year (p < 0.001); and total progressively motile sperm decreased 4.8% per year (p < 0.001). In a group of healthy active men, semen volume, sperm concentration, total sperm count, and sperm motility decrease continuously between 22-80 years of age, with no evidence of a threshold.

Eskenazi, B.; Wyrobek, A.J.; Kidd, S.A.; Moore, L.; Young, S.S.; Moore, D.

2001-12-01

296

77 FR 74555 - Importation of Live Swine, Swine Semen, Pork, and Pork Products; Estonia, Hungary, Slovakia, and...  

Science.gov (United States)

...the United States. Except for semen collected from swine in Denmark, Finland, the Republic of Ireland, Sweden, or the United...of this section with regard to swine semen imported from Denmark, Finland, the Republic of Ireland, Sweden, or the...

2012-12-17

297

A polymerase chain reaction for the detection of Brucella canis in semen of naturally infected dogs.  

Science.gov (United States)

The objective was to evaluate a PCR assay for the detection of Brucella canis in canine semen, comparing its performance with that of bacterial isolation, serological tests and PCR assay of blood. Fifty-two male dogs were examined clinically to detect reproductive abnormalities and their serum was tested by the rapid slide agglutination test, with and without 2-mercaptoethanol (2ME-RSAT and RSAT, respectively). In addition, microbiological culture and PCR assays were performed on blood and semen samples. The findings of the semen PCR were compared (Kappa coefficient and McNemar test) to those of blood PCR, culture of blood and semen, RSAT, and 2ME-RSAT. Nucleic acid extracts from semen collected from dogs not infected with B. canis were spiked with decreasing amounts of B. canis RM6/66 DNA and the resulting samples subjected to PCR. In addition, semen samples of non-infected dogs were spiked with decreasing amounts of B. canis CFU and the resulting suspensions were used for DNA extraction and amplification. Of the 52 dogs that were examined, the following tests were positive: RSAT, 16 (30.7%); 2ME-RSAT, 5 (9.6%); blood culture, 14 (26.9%); semen culture, 11 (21.1%); blood PCR, 18 (34.6%); semen PCR, 18 (34.6%). The PCR assay detected as few as 3.8 fg of B. canis DNA experimentally diluted in 444.9 ng of canine DNA (extracted from semen samples of non-infected dogs). In addition, the PCR assay amplified B. canis genetic sequences from semen samples containing as little as 1.0 x 10(0) cfu/mL. We concluded that PCR assay of semen was a good candidate as a confirmatory test for the diagnosis of brucellosis in dogs; its diagnostic performance was similar to blood culture or blood PCR. Furthermore, the PCR assay of semen was more sensitive than the 2ME-RSAT or semen culture. Examination of semen by PCR should be included for diagnosis of brucellosis prior to natural mating or AI; in that regard, some dogs that were negative on serological and microbiological examinations as well as blood PCR were positive on PCR of semen. PMID:17343907

Keid, L B; Soares, R M; Vasconcellos, S A; Chiebao, D P; Megid, J; Salgado, V R; Richtzenhain, L J

2007-04-15

298

Semen Quality of Holstein and Buffalo Bulls after Filtration using Sephadex Column  

International Nuclear Information System (INIS)

To evaluate the effect of sephadex column filtration technique on semen quality of five Holstein bulls and five Egyptian buffalo bulls. Semen was collected biweekly from each eight weeks. Immediately after collection, semen was extended (37degree C) and filtered using sephadex column-filtration technique. Semen was evaluated for physical semen characteristics including, percentages of sperm motility, live sperm and sperm abnormality as well as sperm cell concentration pre-and post-filtration. Results show that among all physical semen characteristics, only ejaculate semen volume was significantly (P<0.001) higher in Holstein than buffalo bulls, but motility, livability, abnormality, sperm concentration and sperm with intact acrosome did not differ between both species. As a result of filtration, sperm motility and livability increased (P<0.05) by 16.4 and 11.8% in Holstein and by 16.9 and 10.1% in buffalo semen, respectively. Sperm abnormality and concentration reduced (P<0.05) by 2.6 and 3.3% in Holstein and by 2.4 and 3.5% in buffalo semen, respectively. Improvements of live sperm and the reduction in sperm concentration (proportional to the pre-filtration value) were better (P<0.05) in Holstein than buffalo semen (15.5% and %52.4 vs. 13.2 and -49.3%, respectively). Improvement of motility and abnormality did not differ in Holstein (25.4 and %57.8) and buffalo semen (26.6 and ,(%54.5respectively. The present results indicate that using sephadex column filter techniq that using sephadex column filter technique has beneficial effects on improving quality of spermatozoa in both species. (author)

299

Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO South Africa | Language: English Abstract in english Maintaining a successful pig artificial insemination programme depends on a number of factors, including evaluation of semen characteristics. This study compared the efficacy of different extenders on the sperm motility of Kolbroek semen during short term storage at 4 °C and 25 °C. Semen was collect [...] ed from Kolbroek boars using the gloved hand technique and transported to the laboratory for evaluation. Semen was pooled and randomly allocated to four groups and diluted at a ratio of 1:1 (v/v) with Beltsville thawing solution (BTS), Kobidil+, egg yolk citrate (EYC) and non-extended semen (Control). Each extender had two similar semen samples, making a total of eight samples. Extended and non-extended semen were stored at 4 °C and the other samples at 25 °C for 1 h. Data were analyzed using analysis of variance (ANOVA). The total sperm motility of semen stored at 25 °C was higher when semen was extended with BTS and Kobidil+ in comparison to the egg yolk citrate diluent. However, total sperm motility in the non-extended semen did not differ from the BTS and EYC group during storage at 25 °C. Sperm progressive motility was higher in the BTS group, compared to the Kobidil+ and non-extended groups. Sperm motility of Kolbroek semen at 4 °C did not differ between all extender treatments. Total motility rate was significantly higher when Kolbroek sperm were stored at 25 °C than at 4 °C. It can be concluded that Kolbroek sperm, extended with BTS, maintained their motility rate better for short term storage at 25 °C in comparison to 4 °C.

M.H., Mapeka; K.C., Lehloenya; T.L., Nedambale.

300

Survey of carnitine content of human semen using a semiquantitative auxanographic method: decreased semen total carnitine concentration in patients with azoospermia or severe oligozoospermia.  

Science.gov (United States)

A microbiological method, using the carnitine-requiring yeast, Torulopsis bovina ATCC 26014, was developed to identify samples of human semen which contained low levels (less than 250 micron M) of total carnitine. Of 399 semen samples from a male infertility clinic which were tested, 30 (7.5%) were low in carnitine. Of these, 14 were azoospermic and 16 were severely oligozoospermic. Some azoospermic samples (19 = 58%) and severely oligozoospermic samples (51 = 79%) did not give evidence of low carnitine concentrations. These results indicate that decreased total carnitine concentration in semen occurs in certain classes of azoospermic and severely oligozoospermic patients. PMID:7198393

Soffer, Y; Shalev, D P; Weissenberg, R; Orenstein, H; Nebel, L; Lewin, L M

1981-01-01

301

Flora bacteriana del semen de toro antes y después de la congelación (Bacterial flora of bull semen before and after freezing process  

Directory of Open Access Journals (Sweden)

Full Text Available Se investigó por bacteriología general el semen fresco y después de la congelación de 50 toros de inseminación artificial y se efectuó el conteo total de unidades formadoras de colonias (UFC. A l5 de los toros se les realizó el examen bacteriológico de sus lavados prepuciales. En todas las muestras de semen fresco se obtuvo crecimiento bacteriano y los gérmenes más frecuentemente aislados fueron: Escherichia coli (50,0%, Staphylococcus aureus (36,0% y Staphylococcus coagulasa negativa (28,0%. En el semen congelado solamente se obtuvo crecimiento en el 20,0%. El 74,0% del semen fresco alcanzó conteos ? 1 x 104 UFC/mL antes de ser procesado; después de la congelación el 80,0% fue estéril. En el total de lavados prepuciales se obtuvo crecimiento y se detectó en mayor proporción el Staphylococcus coagulasa negativa (60,0%, microorganismo también aislado en el semen fresco de estos toros. Se concluyó que la adición de antibióticos al menstruo y posterior congelación en pastillas, disminuye notablemente la carga microbiana presente en el semen. It was investigated through general bacteriology both fresh semen and after the freezing process, carried out in 50 bulls of artificial insemination, total counting of colony forming units (CFU was made. A bacteriological analysis of the prepucial washing was made on 15 of these bulls. In all samples of fresh semen there was bacterial growing. The most frequently germs were: Escherichia coli (50,0%, Staphylococcus aureus (36,0% and coagulase negative Staphylococcus (28,0%. In samples of frozen semen growth was only obtained in the 20,0%. The 74,0% of samples of fresh semen reached counts ? 1 x 104 CFU/mL before being processed; after freezing 80,0% of the samples were sterile. In all prepucial washings it was obtained growth and mostly detected coagulase negative Staphylococcus (60.0%, was also isolated in the fresh semen of these bulls. We concluded that the addition of antibiotics to the menses and later freezing in pills, diminishes the load microbial present notably in the semen

Enrique A. Silveira Prado

2005-10-01

302

Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender.  

Science.gov (United States)

The aim of this study was to evaluate the effects of ergothioneine and cysteamine as antioxidant supplements in a soybean lecithin extender for freezing ram semen. Twenty-four ejaculates were collected from four rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and cysteamine or ergothioneine (2, 4, 6 or 8mM). Motility by CASA, viability, plasma membrane functionality (HOS test), total abnormality, lipid peroxidation, glutathione peroxidase (GPx) activity and capacitation status (CTC staining) were assessed after thawing. Using 6mM of either antioxidant improved total motility. Cysteamine at 6mM and ergothioneine at 4 and 6mM improved viability and reduced lipid peroxidation (malondialdehyde concentration). Both antioxidants improved membrane functionality significantly, except at 8mM. Progressive motility, kinematic parameters, GPx activity, capacitation status and sperm abnormalities were not influenced by the antioxidant supplements. In conclusion, cysteamine at 6mM and ergothioneine at 4 or 6mM seem to improve the post-thawing quality of ram semen cryopreserved in a soybean lecithin extender. PMID:24854868

Najafi, Abozar; Kia, Hossein Daghigh; Mohammadi, Hossein; Najafi, Mir Hossein; Zanganeh, Zaynab; Sharafi, Mohsen; Martinez-Pastor, Felipe; Adeldust, Hamideh

2014-08-01

303

Detection of boar sperm plasma membrane protein using Rhodamine 640; implications for cryobiology and physiology  

Science.gov (United States)

Rhodamine 640 (R640) was used to detect changes in boar sperm plasma membrane protein (PMP) during cryopreservation; a poorly understood phenomenon. The protocol was adapted for boar sperm so that semen samples (n = 17) could be analyzed for PMP (R640 positive) and plasma membrane integrity (PMI; Y...

304

Evaluation of the effectiveness of semen processing techniques to remove bovine viral diarrhea virus from experimentally contaminated semen samples.  

Science.gov (United States)

The aim of this study was to evaluate the capacity of three semen processing techniques, Percoll gradient centrifugation, Swim-up and a combination of Swim-up and Percoll gradient centrifugation, to reduce the viral load of bovine viral diarrhea virus (BVDV) in experimentally infected semen samples. The evaluation was performed using two approaches: first, searching for the presence of virus in the processed samples (via virus titration and RT-PCR) and second, ascertaining the possible interference on in vitro embryo production. The sperm count and DNA integrity (Comet assay) of the processed samples were analyzed (Experiment 1). The amount of virus in the processed samples was determined by titration in cell culture (Experiment 2). The samples processed by Swim up/Percoll gradient centrifugation were utilized for in vitro embryo production, and the embryos produced were tested for BVDV by RT-PCR (Experiment 3). Sperm concentration, Comet assay and embryo production were analyzed by chi-squared tests (P<0.05). There was a significant difference between sperm separation techniques when the sperm count and Comet assay were analyzed. The sperm count obtained from the Swim up/Percoll gradient centrifugation group was lower than that obtained in either of the two other groups (Swim up and Percoll gradient centrifugation), and the Comet assay showed that the combination of the two semen processing techniques (Swim up/Percoll gradient) produced a 1.1% prevalence of Comet level 2, which was not observed in the other groups. The BVDV titer (10(6.68)TCID(50)/mL) added to experimentally infected semen samples decreased after Percoll gradient centrifugation to 10(2.3)-10(1)TCID(50)/mL; for the Swim up group, the titer range was 10(3.3)-10(1.87)TCID(50)/mL, and in the Swim up/Percoll gradient centrifugation group, BVDV was undetectable. The decreases in titer varied from 99.9% in the Swim up-processed group to 100% in the Swim up/Percoll gradient centrifugation group. In vitro embryo production displayed similar blastocyst development rates among all groups, and RT-PCR was negative for the produced embryos. The data showed that the combination of Swim up/Percoll gradient centrifugation promoted the elimination of BVDV from the semen samples without damaging spermatozoa cells and also allowed successful in vitro embryo production free of BVDV. Hence, the risk of BVDV contamination is negligible for the embryo recipient. PMID:23219984

Galuppo, Andrea G; Junior, Nelson B; Arruda, Nathalia S; Corbellini, Angela O; Chiappetta, Catarina M; Pavão, Danielle L; D'Angelo, Magali; Canal, Cláudio W; Rodrigues, José L

2013-02-01

305

Human semen quality in the new millennium : a prospective cross-sectional population-based study of 4867 men  

DEFF Research Database (Denmark)

Considerable interest and controversy over a possible decline in semen quality during the 20th century raised concern that semen quality could have reached a critically low level where it might affect human reproduction. The authors therefore initiated a study to assess reproductive health in men from the general population and to monitor changes in semen quality over time.

JØrgensen, Niels; Joensen, Ulla Nordström

2012-01-01

306

Effect of sexed-semen use on Holstein conception rate, calf sex, dystocia, and stillbirth in the United States  

Science.gov (United States)

Most artificial-insemination organizations in the United States now market sex-sorted semen. For 10.8 million US Holstein breedings with conventional semen since January 2006 and 122,705 sexed-semen breedings, data were available from all breedings for conception rate, 12 and 9% of breedings for cal...

307

Detection of Ureaplasma spp. in semen samples from sheep in Brazil  

Scientific Electronic Library Online (English)

Full Text Available A study was conducted to verify the presence of mycoplasmas and ureaplasmas DNA in sheep semen samples from the State of Pernambuco. The PCR assay was conducted of according with standard protocols with generic primers. Mollicutes DNA was detected in 26.0% and Ureaplasma spp. in 12.0% of semen sampl [...] es.

Sandra Batista dos, Santos; Orestes Luiz de, Souza Neto; Pedro Paulo Feitosa de, Albuquerque; André da Rocha, Mota; Pomy de Cássia Peixoto, Kim; Érica Paes Barreto Xavier de, Moraes; Elmiro Rosendo do, Nascimento; Rinaldo Aparecido, Mota.

2013-09-01

308

Influence of pre-cryopreservation pH and temperature on boar semen quality  

Science.gov (United States)

The influence of shipping temperature and pH on semen quality parameters could determine the effectiveness of current National Animal Germplasm Program protocols. The purpose of this project is to determine associations between pH, shipping temperature, and boar semen quality: cell size, cell inte...

309

Freezability of Tushin ram semen extended with goat or cow milk based extenders.  

Science.gov (United States)

Cow milk is used as an extender for ram semen cryopreservation. Caseins, the major proteins of milk, appear to provide some protective effect to sperm during cryopreservation. Goat milk has unique casein structure. The aim of this study was to investigate effect of goat milk, as a main semen extender, on freezability of Tushin Ram semen. For this aim, ejaculates from four Tushin rams were collected with artificial vagina and pooled. Pooled semen was separately extended with four different extenders: TRIS based (TRIS), cow skim milk based (CSM) (10 g/100 ml), cow semi-skim milk based (CSSM) and goat semi-skim milk based (GSSM) extenders, containing egg yolk and glycerol. The semen was cryopreserved and stored in liquid nitrogen until examination date. After thawing (at 37°C for 1 min), sperm motility, viability, morphology, acrosome and membrane integrity (HOST) were evaluated. Although, there was not any significant differences between extenders in post-thaw percentage of viable spermatozoa (p>0.05), Tushin ram semen extended with GSSM or CSM extenders had significantly higher post-thaw percentage of progressive motility (25.0% and 30.8% respectively), compared with CSSM and TRIS (7.5% and 14.1% respectively, pram semen extended with GSSM (49.5%) and CSM (51.5%), compared with CSSM (65.7%) and TRIS (60.7%) (pram semen, instead of cow milk and Tris based extenders, as a main extender. PMID:21352385

Ari, U Ç; Kulaksiz, R; Öztürkler, Y

2011-12-01

310

ACTIVITY OF NADH-TETRAZOLIUM REDUCTASE IN RAM SEMEN DURING LIQUID AND CRYOPRESERVATION  

Directory of Open Access Journals (Sweden)

Full Text Available The investigations for the determination of NADH-tetrazolium reductase enzyme system activity in ram semen during liquid and cryopreservation in relation with their viability, were carried out. The semen samples with lower motility of spermatozoa after collection, possess lower values of mean cytochemical coefficient for NADH-tetrazolium reductase activity ( ?<0.5, ?<0.5 after liquid and cryopreservation.

Rossen STEFANOV

2011-01-01

311

Detection of human papillomavirus DNA in semen from patients with intrameatal penile warts.  

OpenAIRE

Fifteen semen specimens from 10 men with intrameatal penile warts attending a genitourinary clinic were tested by Southern blot hybridisation for the presence of human papillomavirus (HPV) DNA. Five specimens were positive for HPV types 6/11. This observation may have implications for screening of semen used for artificial insemination by donor.

Green, J.; Monteiro, E.; Gibson, P.

1989-01-01

312

Uso de dilutores hipertónicos en la criopreservación de semen ovino / Hypertonic extenders in the cryopreservation of ovine semen  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Se evaluó el efecto crioprotector de dos dilutores hipertónicos (Trealosa y Lactosa) sobre las características postdescongelamiento del semen ovino (n=4). La composición de los dilutores base incluyó Tris 27.1 g/l, ácido cítrico 14.0 g/l, fructosa 10.0 g/l, glicina 10.0 g/l, yema de huevo 10.0 % (v/ [...] v) y glicerol 6.5 % (v/v). El semen colectado con vagina artificial tuvo las siguientes características: volumen: 1.1 ± 0.1ml, concentración espermática: 3.5 ± 0.1 x 109/ml, motilidad individual: 87.0 ± 2.4%, motilidad masal (escala 0- 5): 4.4 ± 0.2, espermatozoides vivos: 90.2 ± 3.8% y anormales 1.8 ± 0.7%. El semen fue congelado en pajillas de 0.5 ml y conservado en nitrógeno líquido. Las pajillas fueron descongeladas luego de 3 meses para su evaluación. Se obtuvo una motilidad individual de 40.3 ± 5.9 y 30.0 ± 5.0% y un número de espermatozoides vivos de 34.4 ± 6.6 y 24.4 ± 5.0 para los dilutores Trealosa y Lactosa, respectivamente. El mejor resultado se obtuvo al utilizar el dilutor hipertónico Trealosa por tener mejores características de motilidad individual y espermatozoides vivos postdescongelamiento. Abstract in english The cryoprotectant effect of two hypertonic extenders (trehalose and lactose) on the post-thawing characteristics of ram semen (n=4) was evaluated. The extender composition included Tris 27.1 g/l, Citric acid 14.0 g/l, Fructose 10.0 g/l, Glycine 10.0 g/l, egg yolk 10.0% (v/v) and Glycerol 6.5% (v/v) [...] . Semen was collected in an artificial vagina. Seminal characteristics were: volume: 1.1 ± 0.1 ml, sperm concentration: 3.50 ± 0.1 x 109/ml, individual motility: 87.0 ± 2.4%, wave motility (scale 0-5): 4.4 ± 0.2, live sperms: 90.2 ± 3.8%, and abnormal sperms: 1.8 ± 0.7%. Semen was frozen in 0.5 ml straws and stored in liquid nitrogen. Straws were thawed after 3 months. Results of post-thawing evaluation were: individual motility: 40.3 ± 5.9 and 30.0 ± 5.0%, and live sperms: 34.4 ± 6.6 and 24.3 ± 5.0% for the Trehalose and Lactose extenders respectively. Results showed a better ram semen cryopreservation when the Trehalose extender was used.

Hernán, Guerrero V.; Wilfredo, Huanca L.; Fernando, Raymundo T.; Sandra, Huerta O.; Daphne, Ramos D..

313

Domestic Cat Model for Predicting Human Nucleoside Analogue Pharmacokinetics in Blood and Seminal Plasma  

OpenAIRE

To establish whether a feline model can predict nucleoside analogue behavior in human semen, zidovudine (ZDV) and lamivudine (3TC) pharmacokinetic parameters (PKs) were determined in the blood and seminal plasma of healthy cats. Our results show considerable similarity in ZDV and 3TC PKs between cats and humans. As in humans, ZDV and 3TC tend to accumulate in feline seminal plasma. Area under the blood plasma concentration-time curve was predictive of seminal plasma excretion. The felid model...

Jordan, Holly L.; Pereira, Arlene S.; Cohen, Myron S.; Kashuba, Angela D. M.

2001-01-01

314

Semen parameters in fertile US men: the Study for Future Families.  

Science.gov (United States)

Establishing reference norms for semen parameters in fertile men is important for accurate assessment, counselling and treatment of men with male factor infertility. Identifying temporal or geographic variability in semen quality also requires accurate measurement of semen parameters in well-characterized, defined populations of men. The Study for Future Families (SFF) recruited men who were partners of pregnant women attending prenatal clinics in Los Angeles CA, Minneapolis MN, Columbia MO, New York City NY and Iowa City IA. Semen samples were collected on site from 763 men (73% White, 15% Hispanic/Latino, 7% Black and 5% Asian or other ethnic group) using strict quality control and well-defined protocols. Semen volume (by weight), sperm concentration (hemacytometer) and sperm motility were measured at each centre. Sperm morphology (both WHO, 1999 strict and WHO, 1987) was determined at a central laboratory. Mean abstinence was 3.2 days. Mean (median; 5th-95th percentile) values were: semen volume, 3.9 (3.7; 1.5-6.8) mL; sperm concentration, 60 (67; 12-192) × 10(6) /mL; total sperm count 209 (240; 32-763) × 10(6) ; % motile, 51 (52; 28-67) %; and total motile sperm count, 104 (128; 14-395) × 10(6) respectively. Values for sperm morphology were 11 (10; 3-20) % and 57 (59; 38-72) % normal forms for WHO (1999) (strict) and WHO (1987) criteria respectively. Black men had significantly lower semen volume, sperm concentration and total motile sperm counts than White and Hispanic/Latino men. Semen parameters were marginally higher in men who achieved pregnancy more quickly but differences were small and not statistically significant. The SFF provides robust estimates of semen parameters in fertile men living in five different geographic locations in the US. Fertile men display wide variation in all of the semen parameters traditionally used to assess fertility potential. PMID:24009155

Redmon, J B; Thomas, W; Ma, W; Drobnis, E Z; Sparks, A; Wang, C; Brazil, C; Overstreet, J W; Liu, F; Swan, S H

2013-11-01

315

EFFECT OF TWO DIFFERENT ANTIBIOTIC COMBINATIONS ON FERTILITY OF FROZEN BUFFALO AND SAHIW AL BULL SEMEN  

Directory of Open Access Journals (Sweden)

Full Text Available This study was carried out to identify the suitable antibiotic combinations in semen extender for improvement in fertility of frozen semen of buffalo and cow (Sahiwal bulls to obtain better pregnancy rate through artificial insemination (AI. For this study eight first ejaculates, four each from a buffalo and a cow (Sahiwal bull were used. The ejaculates were split-sampled and diluted with Tris-citric acid extender (at 37°C; 50x 106 spermatozoa/mI, containing either SP (streptomycin 1000 ~g/ml and penicillin 1000 IU/ml or GTLS (gentamycin 500 µg/ml, Tylosin 100 µg/ml and linco-spectin 300/600 µg/ml. There was no difference in post-thaw motility for these samples. Fertility test based on 75-days first service pregnancy rate was determined under field conditions. A total of 400 inseminations were recorded, 200 for each buffalo and cow (Sahiwal with J 00 of each antibiotic combination, respectively. Fertility rates for SP-based frozen semen of buffalo bull were 41.66% and were 55.2% for GTLS-containing frozen semen, respectively. The results for GTLS were higher (P<0.0001 than SP. Similarly, fertility rates were higher (P<0.0001 for GTLS-based frozen semen of Sahiwal bull (78.78% than SP-containing frozen semen (69.6% of the same specie. Fertility rates also differed due to species of donor bulls. They were better (P<0.0001 for the frozen Sahiwal bull semen than that of the buffalo bull in both SP and GTLS- based frozen semen samples, respectively. In conclusion. seminal quality measured by field fertility trial indicated GTLS combination of antibiotics added to the semen extender was better for improvement in the fertility of frozen buffalo and Sahiwal bull semen, by yielding better pregnancy rates through AI.

S.M.H. Andrabi, N. Ahmad, A. Abbas and M. Anzar

2001-09-01

316

Successful artificial insemination in the Asian elephant (Elephas maximus using chilled and frozen-thawed semen  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Artificial insemination (AI using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artificial insemination (AI. Methods Semen samples were collected by from 8 bulls (age range, 12-to 42-years by manual stimulation. Semen with high quality were either cooled to 4°C or frozen in liquid nitrogen (-196°C before being used for AI. Blood samples collected from ten elephant females (age range, 12-to 52-years were assessed for estrus cycle and elephants with normal cycling were used for AI. Artificial insemination series were conducted during 2003 to 2008; 55 and 2 AI trials were conducted using frozen-thawed and chilled semen, respectively. Pregnancy was detected using transrectal ultrasonography and serum progestagen measurement. Results One female (Khod inseminated with chilled semen became pregnant and gave birth in 2007. The gestation length was 663 days and the sex of the elephant calf was male. One female (Sao inseminated with frozen-thawed semen showed signs of pregnancy by increasing progestagen levels and a fetus was observed for 5 months by transrectal ultrasonography. Conclusion This is the first report showing pregnancy following AI with frozen-thawed semen in the Asian elephant. Successful AI in the Asian elephant using either chilled or frozen-thawed semen is a stepping stone towards applying this technology for genetic improvement of the elephant population.

Wongkalasin Warut

2009-07-01

317

Purification of PSA from human semen  

International Nuclear Information System (INIS)

Full text: 1. Human seminal plasma collected from many volunteers are pooled and passed through a column of phenyl sepharose equilibrated with 1.25 M ammonium sulphate. Elution is carried out with 1.25 M ammonium sulphate initially, to remove the bulk non-adsorbing proteins. Gradient elution of the absorbed proteins with 0.01 M Tris-HCl, 0.25 M NaCl, pH 7.0 buffer gives a sharp peak containing PSA. At each stage, PSA has to be identified by an independent method such as immunodiffusion or an immunoassay. 2. The absorbed protein peak containing PSA is then lyophilised, redissolved in Tris-HCl buffer and chromatographed in a Superdex-75 or Sephadex-75 column. The absorbed proteins elute out as multiple peaks and PSA is eluted as a sharp peak.At each stage, PSA has to be identified by an independent method such as immunodiffusion or an immunoassay. 3. Step 2 is repeated for better purity. 4. The PSA peak is lyophilised, dissolved in Tris-HCl buffer without NaCl and further purified on an ion exchange column (either anion or cation exchange columns such as DEAE Sephadex or CM-Sephadex or Mono Q). Gradient elution using Tris-HCl buffer without NaCl and Tris-HCl buffer with 0.25 M NaCl resulted in a sharp pure PSA peak (homogenous, sharp single band on SDS-PAGE). This procedure is based on that reported by Wang et al., Oncology, 39,1,1982

318

Semen characteristics of the captive Indian leopard, Panthera pardus.  

Science.gov (United States)

Semen samples from 11 Indian leopards (Pantherapardus) from 3 different zoos in India were collected by electroejaculation. A computer-aided semen analyzer (CASA) was used for assessing the quality of the semen vis-à-vis sperm motility. The volume of the ejaculate, sperm density, and the number of motile and morphologically normal spermatozoa were found to be 1.57 +/- 1.26 mL, 55.78 million +/- 38.67 million per mL, 57.05% +/- 16.96% and 71.92% +/- 15.32%, respectively. Although the spermatology varied between individuals in the study, Box-Whisker-plot analysis suggested that the distribution was normal (P > .05). The ejaculated sperm were cryopreserved after diluting in test-yolk buffer. The post-thaw motility was 32.14% and did not differ at 30 or 60 days after cryopreservation. CASA indicated that the progressive velocity (VSL) of cryopreserved spermatozoa was decreased and, as a consequence, they moved more slowly than the neat (VSL 76.3 microm/sec in neat vs 53.8 microm/sec in cryopreserved spermatozoa) and the trajectories were less planar. However, both cryopreserved and neat spermatozoa penetrated the zona-free hamster oocyte with equal efficiency (79% neat vs 80% cryopreserved). The study also reports application of CASA for feline spermatozoa and provides information for the first time on the spermatology of the Indian leopard. This baseline data could be used in captive breeding programs. The results are compared and discussed with the available information on other felines. PMID:11191084

Jayaprakash, D; Patil, S B; Kumar, M N; Majumdar, K C; Shivaji, S

2001-01-01

319

VIABILIDAD ESPERMÁTICA E INTEGRIDAD DEL ACROSOMA EN SEMEN CONGELADO DE TOROS NACIONALES / SPERM VIABILITY AND ACROSOME INTEGRITY IN NATIONAL FROZEN BULL SEMEN  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Se evaluó el deterioro de la membrana espermática e integridad del acrosoma como método para predecir la fertilidad en toros. Se trabajó con cuatro toros (2 Hosltein y 2 Brown Swiss) del Banco Nacional de Semen, Lima-Perú. Se evaluó integridad acrosomal, integridad de membrana espermática, motilidad [...] , espermatozoides vivos, volumen y concentración durante los procesos de refrigeración, congelación y descongelación de 10 eyaculados por animal. En semen fresco sin diluir se encontró un volumen de 4.33 ml, concentración espermática de 922.5 x 106/ml, y 78.5% de espermatozoides vivos. La motilidad individual progresiva en semen diluido fue de 82.7 a 86.0% con diferencia significativa entre toros (p Abstract in english The deterioration of the sperm membrane and acrosome integrity as a method for predicting fertility in bulls was evaluated. Four bulls (2 Holstein and 2 Brown Swiss) from the National Bank of Semen, Lima-Peru were used. Acrosome integrity, sperm membrane integrity, motility, live sperm cells, volume [...] , and sperm concentration during cooling, freezing and thawing was evaluated in 10 ejaculates per sire. In fresh semen, volume was 4.33 ml; sperm concentration was 922.5 x 106/ml and 78.5% of live cells. The individual progressive motility in diluted semen was 82.7 to 86.0% with significant difference between bulls (p

Próspero, Cabrera V; César, Pantoja A.

320

CORRELATION BETWEEN HYPO-OSMOTIC SWELLING TEST AND VARIOUS CONVENTIONAL SEMEN EVALUATION PARAMETERS IN FRESH NILI-RAVI BUFFALO AND SAHIWAL COW BULL SEMEN  

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Full Text Available The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates from each bull were collected. Each semen sample was divided into two parts. One part was used for the evaluation of semen by conventional method, while the other part was subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a significant (P<0.05 positive correlation between progressive motility, morphologically normal spermatozoa, sperm viability and percentage of HOS test positive spermatozoa for both species. It was inferred that HOS test could be a valuable method for routine evaluation of semen for artificial insemination.

L. A. LODHI, M. ZUBAIR, Z. I. QURESHI, I. AHMAD AND H. JAMIL

2008-12-01

321

Supplementation of different concentrations of Orvus Es Paste (OEP) to ostrich egg yolk lipoprotein extender improves post-thaw boar semen quality.  

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This study aimed to compare post-thaw quality of boar semen following freezing in an ostrich egg yolk lipoprotein (LPFo) extender supplemented with 0%, 0.25% and 0.50% Orvus Es Paste (OEP). Sperm assessments included total motility (TMOT), mitochondrial function (MF), plasma membrane integrity (PMI) and acrosome integrity (normal apical ridge, NAR). Considerable variations among boars and OEP treatments had a significant effect (P ostrich egg yolk lipoproteins, could have varying effects on post-thaw sperm survival. PMID:24988847

Fraser, L; Jasiewicz, E; Kordan, W

2014-01-01

322

Single layer centrifugation (SLC) improves sperm quality of cryopreserved Blanca-Celtibérica buck semen.  

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The aim of the present study was to evaluate the effect of sperm selection by means of single layer centrifugation (SLC) on sperm quality after cryopreservation, either when SLC is used before freezing or after thawing, using Blanca-Celtibérica buck semen collected by electroejaculation (EE). Ejaculates from six bucks were collected by EE and divided into two aliquots. One of them (unselected) was diluted with Biladyl(®) by the two-step method and frozen over nitrogen vapor. The other aliquot was selected by the SLC technique and subsequently frozen in the same way as the unselected samples (SLC before freezing). In a further treatment, two unselected straws were thawed and SLC was carried out (SLC after thawing). At thawing, sperm motility of all samples ((i) unselected; (ii) selected before freezing and (iii) selected after thawing) was evaluated by CASA. In addition, integrity of the plasma membrane, mitochondrial membrane potential, ROS production and DNA fragmentation index were assessed by flow cytometry. Most of the sperm parameters were improved (P?0.001) in samples selected by SLC after thawing in relation to unselected or selected by SLC before freezing. The percentage of progressive motile spermatozoa was greater (86%) for sperm samples selected after thawing compared with unselected (58%) or selected before freezing (54%). Moreover, percentages of spermatozoa with intact plasma membrane and spermatozoa with high mitochondrial membrane potential (hMMP) were also greater for sperm samples selected after thawing compared to sperm samples unselected or selected before freezing (spermatozoa with intact plasma membrane: 80% vs. 32% vs. 12%; spermatozoa with hMMP: 54% vs. 1% vs. 15%; respectively). Therefore, sperm quality after cryopreservation is improved in Blanca-Celtibérica buck ejaculates collected by EE when a sperm selection technique such as SLC is carried out after thawing. PMID:23084569

Jiménez-Rabadán, P; Morrell, J M; Johannisson, A; Ramón, M; García-Álvarez, O; Maroto-Morales, A; Alvaro-García, P J; Pérez-Guzmán, M D; Fernández-Santos, M R; Garde, J J; Soler, A J

2012-12-01

323

Analysis of hygienic critical control points in boar semen production.  

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The present study addresses the microbiological results of a quality control audit in artificial insemination (AI) boar studs in Germany and Austria. The raw and processed semen of 344 boars in 24 AI boar studs were analyzed. Bacteria were found in 26% (88 of 344) of the extended ejaculates and 66.7% (18 of 24) of the boar studs. The bacterial species found in the AI dose were not cultured from the respective raw semen in 95.5% (84 of 88) of the positive samples. These data, together with the fact that in most cases all the samples from one stud were contaminated with identical bacteria (species and resistance profile), indicate contamination during processing. Microbiological investigations of the equipment and the laboratory environment during semen processing in 21 AI boar studs revealed nine hygienic critical control points (HCCP), which were addressed after the first audit. On the basis of the analysis of the contamination rates of the ejaculate samples, improvements in the hygiene status were already present in the second audit (P = 0.0343, F-test). Significant differences were observed for heating cabinets (improvement, P = 0.0388) and manual operating elements (improvement, P = 0.0002). The odds ratio of finding contaminated ejaculates in the first and second audit was 1.68 (with the 95% confidence interval ranging from 1.04 to 2.69). Furthermore, an overall good hygienic status was shown for extenders, the inner face of dilution tank lids, dyes, and ultrapure water treatment plants. Among the nine HCCP considered, the most heavily contaminated samples, as assessed by the median scores throughout all the studs, were found in the sinks and/or drains. High numbers (>10(3) colony-forming units/cm(2)) of bacteria were found in the heating cabinets, ejaculate transfer, manual operating elements, and laboratory surfaces. In conclusion, the present study emphasizes the need for both training of the laboratory staff in monitoring HCCP in routine semen production and audits in such AI centers for the external control of hygiene parameters. PMID:25459424

Schulze, M; Ammon, C; Rüdiger, K; Jung, M; Grobbel, M

2015-02-01

324

Evaluation of Bulls Based on Semen Quality and Herd Fertility  

OpenAIRE

The data of the present study were collected from Bangladesh Milk Producers` Co-operative Union Limited (BMPCUL) at Baghabarighat, Sirajgong, Bangladesh to evaluate the bull performance through semen quality and 30-day Non Return Rate (NRR). Data on 245 ejaculates and 12,750 services of almost same aged of 5 Sahiwal bulls (B1, B2, B3, B4 and B5) over a period of 5 years were collected from Animal Breeding Section and Cattle Feed Unit of BMPCUL. The significant (p<0.01) individual sire effe...

Mostari, M. P.; Rahman, M. G. M.; Khandoker, M. A. M. Y.; Husain, S. S.

2004-01-01

325

Human immunodeficiency virus type-1 episomal cDNA in semen  

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Full Text Available Abstract Background Episomal 2-long terminal repeat (LTR HIV-1 cDNA, a by-product of HIV-1 infection, is used in clinical trials as a marker for ongoing viral replication. It would be useful to employ 2-LTR cDNA to monitor cryptic HIV-1 infection in the genital tract of men on antiretroviral therapy (ART to predict the evolution of sexually transmissible drug-resistant HIV-1, but studies thus far have failed to detect this marker in semen. The objectives of this study were: 1 to use a technique that maximizes DNA recovery from HIV-1 infected white blood cells in semen to determine if episomal 2-LTR cDNA is detectable in semen of ART-naïve men with other evidence of genital tract HIV-1 infection, and 2 to compare levels of HIV-1 2-LTR cDNA, RNA, and proviral DNA in semen from HIV-1+ men on ART. Results Using a somatic cell DNA extraction technique, 2-LTR cDNA was detected by PCR/ELISA in 4 out of 8 semen samples from ART-naïve men selected for other signs of seminal HIV-1 infection (positive controls. Southern blot and DNA sequencing confirmed that the amplified sequences were HIV-1 2-LTR cDNA; copy numbers ranged from 55 to 504 copies/sample. Two semen samples from a cohort of 22 HIV-1-infected men on dual nucleoside therapy, one with and one without detectable seminal HIV-1 RNA, were 2-LTR cDNA positive (336 and 8,560 copies/sample. Following addition of indinavir to the therapy regimen, no semen samples from 21 men with controlled peripheral and seminal viral loads were 2-LTR cDNA positive at 1 and 6 month time points, despite the persistence of HIV-1 proviral DNA+ semen cells and seminal cytomegalovirus (CMV shedding in some cases. However, one individual who failed indinavir therapy and later developed distinct protease inhibitor (PI drug resistance mutations in semen, maintained elevated levels of HIV-1 RNA and 2-LTR cDNA in semen. Conclusion 2-LTR HIV-1 cDNA is detectable in semen of HIV-1-infected men. Two men on ART had 2-LTR HIV-1 cDNA in semen, suggesting that this marker may prove to be useful to monitor HIV-1 infection in the genital tract of men on ART to predict the evolution of drug resistance mutations in semen.

Mayer Kenneth H

2005-10-01

326

A Study of a Method to Assess the Purity of Sorted Bovine Semen Using Rapid Single-Sperm Sexing PCR  

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Full Text Available Sort reanalysis using flow cytometry is the most common method for determining the purity of X or Y enriched semen. The high cost of this technique (including the required expensive, proprietary machine limits efforts to improve the technique and to promote develop applications for the sorted semen. In this study, the sperm sex (the presence of the X or Y chromosome was identified by both rapid PCR and flow cytometry reanalysis. The rapid PCR results showed that the percentages of X and Y sperm were 48 and 52% in unsorted semen, 92 and 8% in X-enriched semen and 17 and 83% in Y-enriched semen, respectively. Reanalysis of the DNA content of the sorted samples revealed that the X and Y sperm frequencies were 92 and 8% in X-enriched semen and 15 and 85% in Y-enriched semen, respectively. The sex ratio of unsorted semen analyzed by PCR did not significantly deviate from the expected ratio of 1:1 and there was no significant difference between the sex ratios of sorted semen samples determined by PCR and flow cytometry reanalysis. These results indicate that we have established an effective, reliable and rapid PCR method to verify the purity of sorted semen. This method should contribute greatly to the improvement of sperm sorting techniques and the development of applications for sorted semen.

Weihua Du

2011-01-01

327

Toxic metals signature in the human seminal plasma of Pakistani population and their potential role in male infertility.  

Science.gov (United States)

Aims of this study were to provide firsthand data on the incidence of trace metals in human seminal plasma and find possible correlations between levels of toxic metals and semen quality of Pakistani population. Human semen samples were collected from male partners of couples undergoing infertility assessment at the National Institute of Health Islamabad (Pakistan). We investigated seventy-five seminal plasma samples, which were further categorized into three groups (normozoospermia, oligozoospermia and azoospermia) according to WHO guidelines. The concentration of 17 different toxic metals in human seminal plasma was determined simultaneously by using Inductively coupled plasma mass spectrometry (ICP-MS). Out of 17 trace metals, Cd and Ni showed significant difference (p < 0.05) among three monitored groups. Ni and Cd concentrations in the seminal plasma were negatively correlated with sperm concentration (r = -0.26, -0.29) and motility (r = -0.33, -0.37), respectively. This study suggested that exposure of Ni and Cd is mainly related with the consumption of contaminated dietary items, including ghee (cooking oil), flour and other agri-products. In some semen samples, the concentrations of Sn, V, Cu, Pb, Cr and Hg exhibited high levels suggesting a recent human exposure to surrounding sources. In Pakistani human semen samples, the levels of trace metals were lower and/or comparable to that found in populations of other countries. The results show the first evidence of the effect of toxic metals on semen quality and male infertility in Pakistan. PMID:25471479

Zafar, Ambreen; Eqani, Syed Ali Musstjab Akber Shah; Bostan, Nazish; Cincinelli, Alessandra; Tahir, Faheem; Shah, Syed Tahir Abbas; Hussain, Alamdar; Alamdar, Ambreen; Huang, Qingyu; Peng, Siyuan; Shen, Heqing

2014-12-01

328

Motility and fertility of cryopreserved semen in Persian sturgeon, Acipenser persicus, stored for 30-60 min after thawing.  

Science.gov (United States)

We investigated the effect of storage times of frozen-thawed Persian sturgeon (Acipenser persicus) semen on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30, and 60 min at 4°C post-thawing. Frozen thawed semen analyzed immediately after thawing had similar quality characteristics as fresh semen. For cryopreserved semen stored for 30 min after thawing the characteristics did not differ to fresh semen and cryopreserved semen. For cryopreserved semen stored for 60 min a significant decline in the parameters was observed. Fertilization and hatching rates were not affected by storage times of maximally 30 min of storage. PMID:25445465

Aramli, Mohammad Sadegh; Nazari, Rajab Mohammad

2014-12-01

329

Evaluation of Semen Fertility of Bulls by Non-return Rate at 60 Days of Cows under Artificial Insemination Programme in Bangladesh  

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Full Text Available The present study were to evaluate the effect of individual bull, semen types, quality of bull semen, sources of semen on Non-return Rate (NRR at 60 days of cows under field condition. A total 75550 cows were inseminated with 71 bull semens from Central Cattle Breeding Station and Dairy Farm (CCBSDF, Savar, Dhaka, Rajshahi Dairy and Cattle Improvement Farm (RDCIF, Rajabarihat and District Artificial Insemination Centre (DAIC, Rajshahi under 40 Artificial Insemination (AI sub-centres/points of District AI centre, Rajshahi. The overall NRR was obtained 78.54% with chilled and frozen semen produced from three AI centres/stations. Analysis of variance showed that individual bull semen had significant (p<0.05 effect on NRR at 60 days after first insemination. Semen types, quality of bull semen and sources of semen had significant (p<0.001 effect on NRR at 60 days of cows. The significant (p<0.001 highest NRR (82.32% was with chilled semen and lowest was with frozen semen (76.39%. The significant (p<0.001 maximum NRR (83.12% was for the best quality bull semen and minimum (70.13% for the poor quality bull semen. Significant (p<0.001 higher NRR (82.32% was in semen from DAIC, Rajshahi and lower (73.01% in semen from RDCIF, Rajabarihat. Results suggested that the NRR of cows at 60 days after first insemination under field condition may be a good practice to discard poor fertility semen among the individual bull semen, semen types (chilled and frozen, quality of bull semen (poor, good and best and sources of semen (CCBSDF, Savar, RDCIF, Rajabarihat and DAIC, Rajshahi for artificial insemination programme in Bangladesh.

M.J.U. Sarder

2006-01-01

330

Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa.  

Science.gov (United States)

The purpose of the current study was to evaluate the effects of cysteine (C) and glutathione (G) on the post-thawed ram sperm quality. Collected semen samples from four mature rams were diluted with five soybean lecithin (SL)-based extenders containing: no antioxidant (SL-0), 5 mM cysteine (SL-C5), 10 mM cysteine (SL-C10), 5 mM glutathione (SL-G5) and 10 mM glutathione (SL-G10). After freeze-thawing process, motion and velocity parameters, plasma membrane integrity and functionality, morphological abnormality, lipid peroxidation, acrosomal status, mitochondria activity, and apoptosis status of post-thawed ram spermatozoa were assessed. The results showed that SL-C10 increased the total motility and plasma membrane integrity (p ram spermatozoa (55.86 ± 1.37 and 60.57 ± 1.34 %) compared to other extenders. Progressive motility was significantly higher in SL-C10 (24.71 ± 1.13 %) compared to SL-0 (20 ± 1.13 %) and SL-G10 (15 ± 1.13 %). Mitochondrial activity was significantly higher in SL-C10 (56.83 ± 2.29 %) compared to SL-G10 (38.75 ± 2.29 %). Capacitation and acrosomal status, lipid peroxidation, and the percentage of dead spermatozoa were not affected by different extenders. The percentage of live spermatozoa was higher in SL-C10 (56.33 ± 1.35 %) compared to other extenders. Also, SL-C10 resulted in a lower percentage of apoptotic spermatozoa (14.17 ± 0.53 %) compared to other extenders. The results of this study showed that supplementation of SL-based ram semen extender with 10 mM cysteine resulted in an improved quality of post-thawed ram spermatozoa. PMID:24907919

Sharafi, Mohsen; Zhandi, Mahdi; Akbari Sharif, Abbas

2014-06-01

331

Differential protein profile in sexed bovine semen: shotgun proteomics investigation.  

Science.gov (United States)

The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein repertoire for the development of improved methods to differentiate X and Y sperm cells maintaining high vitality. In order to address this issue, we performed a comparative shotgun proteomic investigation by nUPLC-MS/MS to characterize sexed bovine semen. The protein profiles of these two types of sperm cells have shown differential expression of proteins that may be directly associated with the main components of cytoskeletal structures of flagellum, as the axoneme, outer dense fibers and fibrous sheath, as well as glycolytic enzymes and calmodulin, involved in the energetic metabolism regulation. Overall these results may provide a base to a better comprehension of the biological features of sperm cells and may be useful to the development of alternative methods of separation. PMID:24226273

De Canio, Michele; Soggiu, Alessio; Piras, Cristian; Bonizzi, Luigi; Galli, Andrea; Urbani, Andrea; Roncada, Paola

2014-06-01

332

Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions.  

Science.gov (United States)

Freshly collected boar spermatozoa are sensitive to a fast reduction in temperature because of lipid phase transition and phase separation processes. Temperature management during semen processing may determine the quality of stored samples. The aim of this study was to evaluate the influence of isothermic and hypothermic semen processing protocols on boar sperm quality under laboratory and field conditions. In the laboratory study, ejaculates (n = 12) were first diluted (1:1) with Beltsville Thawing Solution (BTS) at 32 °C, then processed either with isothermic (32 °C) or hypothermic (21 °C) BTS, stored at 17 °C, and assessed on days 1, 3, and 6. Temperature curves showed that 150 minutes after the first dilution, semen doses of both groups reached the same temperature. Two-step hypothermic processing resulted in lower sperm motility on days 1 and 6 (P semen samples from each of 23 European artificial insemination studs were evaluated as part of an external quality control program. Semen quality based on motility, membrane integrity, mitochondrial activity, and a thermoresistance test was higher for stations using one-step isothermic dilutions (n = 7) compared with artificial insemination centers using two-step hypothermic protocols (n = 16). Both studies show that chilling injury associated with hypothermic dilution results in lower quality of stored boar semen compared with isothermic dilution and that the type of semen extender affects the outcomes. PMID:23987989

Schulze, M; Henning, H; Rüdiger, K; Wallner, U; Waberski, D

2013-12-01

333

Prevalence of chlamydiae in boars and semen used for artificial insemination.  

Science.gov (United States)

Although there are indications for venereal transmission of chlamydiae in pigs, direct diagnostic evidence on the presence of these bacteria in boars and boar semen in particular is still incomplete. We investigated boars from two studs (A, B) in semen (A: n = 174; B: n = 100) and faeces (A: n = 174; B: n = 24) for chlamydiae using ompA-PCR and partial ompA gene sequencing. Additionally, blood serum was examined for chlamydial antibodies using an indirect ELISA (A: n = 171; B: n = 62). Chlamydiae were found in 9 (5.2%) and 24 (24.0%) semen specimens, and in 71 (40.1%) and 2 (8.3%) faecal samples from boars of stud A and B, respectively. Regarding individual chlamydial species, Chlamydophila psittaci and Chlamydia suis were identified most frequently, with the former predominating in semen (in 23 out of 33 positive samples) and the latter in faeces (68/73). In contrast, Chlamydophila pecorum was found only sporadically. Chlamydial antibodies were detected in 80 (46.8%) and 6 (9.7%) boars of stud A and B, respectively. No correlation was observed between the data from serology and PCR of semen or faeces in either of the studs. In conclusion, detection of chlamydiae in semen of boars suggests a potential for venereal transmission. Whether the high overall prevalence of chlamydial infections reflects a general situation in boars needs to be investigated. Serological testing failed to identify boars shedding chlamydiae in their semen. PMID:16280160

Kauffold, Johannes; Melzer, Falk; Henning, Klaus; Schulze, Katrin; Leiding, Claus; Sachse, Konrad

2006-06-01

334

Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction  

Science.gov (United States)

Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted semen is tedious and expensive. In this study, a rapid, cost effective and reliable method for determining the sex ratio was developed using a multiplex real-time polymerase chain reaction (PCR) assay. In this assay, the X and Y chromosome-specific markers, i.e., bovine proteolipid protein (PLP) gene and sex-determining region Y (SRY) were simultaneously quantified in a single tube. The multiplex real-time PCR assay was shown to have high amplification efficiencies (97% to 99%) comparable to the separated-tube simplex real-time PCR assay. The results obtained from both assays were not significantly different (p>0.05). The multiplex assay was validated using reference DNA of known X ratio (10%, 50%, and 90%) as templates. The measured %X in semen samples were the same within 95% confidence intervals as the expected values, i.e., >90% in X-sorted semen, <10% in Y-sorted semen and close to 50% in the unsorted semen. The multiplex real-time PCR assay as shown in this study can thus be used to assess purity of sex-sorted semen. PMID:25178292

Khamlor, Trisadee; Pongpiachan, Petai; Sangsritavong, Siwat; Chokesajjawatee, Nipa

2014-01-01

335

Semen quality during vincristine treatment in dogs with transmissible venereal tumor.  

Science.gov (United States)

The aim of this study was to evaluate the direct effects of vincristine on semen quality in dogs with transmissible venereal tumor (TVT). We examined the semen of 17 dogs suffering from TVT during vincristine treatment. Each animal received 0.6 mg, i.v. vincristine sulphate per square meter of body surface, per week for 4 wk until complete regression of the tumor. The following semen parameters were evaluated: semen volume (second fraction), sperm concentration, total spermatozoa per ejaculate, percentage of progressively motile spermatozoa, percentage of dead spermatozoa, percentage of swollen spermatozoa (hypo-osmotic swelling test) and percentage of morphologically abnormal spermatozoa (primary and secondary defects). Semen was collected and evaluated prior to the beginning of treatment, 3 d after each vincristine injection and 15 d after the last injection. Semen characteristics transiently deteriorated during treatment, but returned to normal 15 d later. These changes were attributed to a direct effect of vincristine on the extragonadal spermatozoal reserves contained in the epididymis and ductus deferens. A GnRH stimulation test was also performed after each semen collection in order to assess the function of the hypothalamic-pituitary-Leydig cell axis. No effect was noted on the above axis. PMID:10798495

Saratsis, P; Ypsilantis, P; Tselkas, K

2000-03-15

336

Viral RNA load in semen from bluetongue serotype 8-infected rams: relationship with sperm quality.  

Science.gov (United States)

This study investigated if viral RNA was detectable in the semen of rams clinically infected with bluetongue virus serotype 8 (BTV-8) by RT-qPCR, and to what extent the amount detected may be predictive of sperm quality. Semen samples were collected on six occasions from 93 BTV-8 infected rams involved in two longitudinal (n=12 and 27, respectively) and one cross-sectional (n=54) field study. Semen quality was assessed in terms of mass motility, concentration of spermatozoa, percentage of living and dead spermatozoa as well as cytological features. An overall semen quality score (SQS) was established. Depending upon the studied population, BTV RNA was detected in 75-100% of semen samples at initial testing 25-57 days post-observation (DPO) of clinical signs, and was detectable up to 116 DPO in a proportion of rams undergoing repeated sampling. Semen quality variables were significantly altered following natural BTV-8 infection and correlated with the amount of BTV RNA present. The SQS did not return to normal when virus was no longer detectable, suggesting that clearance of BTV precedes full recovery of sperm quality. In conclusion, viral RNA may be transiently recovered from the semen of BTV-8 affected rams and may serve as an indicator in predicting ram breeding potential following natural infection. PMID:21802323

Leemans, Jérôme; Raes, Marianne; Vanbinst, Tine; De Clercq, Kris; Saegerman, Claude; Kirschvink, Nathalie

2012-06-01

337

Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction.  

Science.gov (United States)

Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted semen is tedious and expensive. In this study, a rapid, cost effective and reliable method for determining the sex ratio was developed using a multiplex real-time polymerase chain reaction (PCR) assay. In this assay, the X and Y chromosome-specific markers, i.e., bovine proteolipid protein (PLP) gene and sex-determining region Y (SRY) were simultaneously quantified in a single tube. The multiplex real-time PCR assay was shown to have high amplification efficiencies (97% to 99%) comparable to the separated-tube simplex real-time PCR assay. The results obtained from both assays were not significantly different (p>0.05). The multiplex assay was validated using reference DNA of known X ratio (10%, 50%, and 90%) as templates. The measured %X in semen samples were the same within 95% confidence intervals as the expected values, i.e., >90% in X-sorted semen, <10% in Y-sorted semen and close to 50% in the unsorted semen. The multiplex real-time PCR assay as shown in this study can thus be used to assess purity of sex-sorted semen. PMID:25178292

Khamlor, Trisadee; Pongpiachan, Petai; Sangsritavong, Siwat; Chokesajjawatee, Nipa

2014-10-01

338

Variation in lipid profiles within semen compartments--the bovine model of aging.  

Science.gov (United States)

Semen lipid composition was examined in young and mature bulls. Given the specific roles of various semen compartments (i.e., seminal fluid, sperm head, and sperm tail) during fertilization, we hypothesized that altered fatty acid and cholesterol composition of a specific compartment might impair semen quality and sperm function. Semen samples were collected from five mature and five young Holstein Friesian bulls during the winter (December-January). Semen was evaluated by computerized sperm-quality analyzer for bulls and was centrifuged to separate the sperm from the seminal fluid. The sperm fraction was sonicated to separate its head and tail compartments. Cold extraction of lipids was performed, and fatty acids and cholesterol were identified and quantified by gas chromatography. Semen physiological features (concentration, motility, and progressive motility) did not differ between mature and young bulls. However, lipid composition within fractions varied between groups, with prominent impairments in the head compartment. In particular, the proportions of polyunsaturated fatty acids, omega-3 fatty acids, and docosahexaenoic acid in the intact sperm; seminal fluid; and sperm head were lower in semen collected from mature bulls than in that from young bulls. The finding suggests an age-differential absorption and/or metabolism through spermatogenesis. Reduced proportions of major fatty acids in mature bulls might reduce membrane fluidity, which in turn might affect the ability to undergo cryopreservation and/or oocyte-sperm fusion through fertilization. PMID:23830232

Argov-Argaman, Nurit; Mahgrefthe, Karin; Zeron, Yoel; Roth, Zvi

2013-10-15

339

Season-induced variation in lipid composition is associated with semen quality in Holstein bulls.  

Science.gov (United States)

Season-induced variation in fatty acid and cholesterol composition in bovine semen has been associated with semen quality. Given the specific roles of the various semen compartments (seminal fluids, sperm head, and sperm tail) in fertilization, we hypothesized that environmental-stress-induced alterations in the lipid composition of a specific compartment might impair semen quality and sperm function. Semen samples were collected from five mature Holstein-Friesian bulls during the summer (August to September) and winter (December to January). Semen was evaluated by computerized sperm-quality analyzer, calibrated for bulls' semen, and centrifuged to separate the spermatozoa from the seminal fluids. The spermatozoal fraction was sonicated to separate the sperm head and tail compartments. Cold lipid extraction was performed with chloroform:methanol (2:1, vol/vol). Lipids were identified and quantified by gas chromatography. Seasonal variation was found in both physiological and structural parameters. The proportion of spermatozoa defined as morphologically normal was higher in the winter, with higher motility, progressive motility, and velocity relative to summer samples. Lipid composition within fractions varied between seasons with prominent impairment in the tail compartment, characterized by high saturated fatty acid, low polyunsaturated fatty acid, and low cholesterol concentrations during the summer. Given the association between alterations in lipid composition and reduced sperm motility and velocity during the summer, it is suggested that lipid composition might serve to predict sperm quality. PMID:23630332

Argov-Argaman, N; Mahgrefthe, K; Zeron, Y; Roth, Z

2013-05-01

340

Spermatozoal morphologies and fructose and citric acid concentrations in agouti (Dasyprocta leporina) semen.  

Science.gov (United States)

This study was conducted to identify the levels of fructose and citric acid, and sperm morphologies in agouti (Dasyprocta leporina) semen. These parameters may be important in identifying highly fertile semen from the agouti. The objectives were: (1) to investigate spermatozoal abnormalities in agouti semen and (2) to determine the concentrations of seminal fructose and citric acid in agouti semen samples. Semen samples were collected from 16 anaesthetised male agouti by electro-ejaculation. Fructose and citric acid concentrations were 256.86+/-63.54 mg/dl and 1877+/-147 mg/dl, respectively, measured with ELISA kits. Sperm morphologies, examined using eosin-negrosin staining, showed 11 morphologies. The most abundant (68.5%) sperm morphology (M1) showed no known sperm defects. Means for head, mid piece, tail and total length of the agouti spermatozoa was 5.23+/-0.04 microm, 5.18+/-0.08 microm, 37.52+/-0.24 microm and 47.96+/-0.25 microm, respectively for M1 sperm. The means of spermatozoa head and mid piece width and semen volume were 3.26+/-0.04 microm, 0.70+/-0.02 microm and 0.47+/-0.16 ml, respectively. It was concluded that as the fructose concentration in agouti ejaculate increased the percentage of spermatozoa with known spermatozoa defects increased (r=0.506; Pagouti ejaculate. In conclusion standards for identifying fertile agouti semen were established. PMID:17919856

Mollineau, W M; Adogwa, A O; Garcia, G W

2008-05-01

341

Effect of the Type of Straw on the Spermatic Quality in the Freezing of Boar Semen  

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Full Text Available With the freezing boar semen, could have better options for the optimization of the reproductive handling in the swinish species as well as an alternative for the development of this cattle activity; using technologies like the implementation of banks of frozen of races with characteristic zootechnic of economic importance that guarantee the readiness of germinal material in the moment that is required, to have germinal material of males proven genetically, still when the animal no longer exists, to overcome certain intentional restrictions of transport of alive animals, for the problem of transmission of illnesses and, to overcome the restrictive of time of viability of the diluted fresh semen. In this work was examined the effect of the freezing boar semen in straws plastic of 0.5 and 5 mL on the Motility and the Acrosome Integrity (NAR. For it, 9 were used ejaculated of different animals, the experiment was carried out comparing fresh semen with thawing semen coming from straws of 0.5 and 5 mL. The results of percentages of motility and NAR for fresh and thawing semen, were of 86.19, 47.14 and 47.14, for straws of 0.5 mL and 75.62, 48.19 and 46.81, for straws of 5 mL. When carrying out the analysis of the variance and the test of multiple comparisons it was found that the freezing of the semen in both straws types, the percentages of motility and NAR reduce, with regard to the fresh semen; however, the macrotubes or straws of 5 mL, represent a good option in the artificial insemination using boar semen frozen-thawing.

C.A. C?rdova-Jim?nez

2006-01-01

342

An Outbreak of Porcine Reproductive and Respiratory Syndrome Virus in Switzerland Following Import of Boar Semen.  

Science.gov (United States)

An outbreak of porcine reproductive and respiratory syndrome virus (PRRSV) occurred in November 2012 in Switzerland (CH), traditionally PRRSV-free. It was detected after a German boar stud informed a semen importer about the detection of PRRSV during routine monitoring. Tracing of semen deliveries revealed 26 Swiss sow herds that had used semen from this stud after its last negative routine monitoring and 62 further contact herds. All herds were put under movement restrictions and examined serologically and virologically. As a first measure, 59 sows from five herds that had previously been inseminated with suspicious semen were slaughtered and tested immediately. Investigations in the stud resulted in 8 positive boars with recent semen deliveries to CH (Seven with antibodies and virus, one with antibodies only). In one boar out of six tested, virus was detected in semen. Of the 59 slaughtered sows, five from three herds were virus-positive. In one herd, the virus had spread, and all pigs were slaughtered or non-marketable animals euthanized. In the remaining herds, no further infections were detected. After confirmatory testings in all herds 3 weeks after the first examination gave negative results, restrictions were lifted in January 2013, and Switzerland regained its PRRSV-free status. The events demonstrate that import of semen from non-PRRS-free countries - even from negative studs - poses a risk, because monitoring protocols in boar studs are often insufficient to timely detect an infection, and infections of sows/herds occur even with low numbers of semen doses. The outbreak was eradicated successfully mainly due to the high disease awareness of the importer and because immediate actions were taken before clinical or laboratory diagnosis of a single case in the country was made. To minimize the risk of an introduction of PRRSV in the future, stricter import guidelines for boar semen have been implemented. PMID:25209832

Nathues, C; Perler, L; Bruhn, S; Suter, D; Eichhorn, L; Hofmann, M; Nathues, H; Baechlein, C; Ritzmann, M; Palzer, A; Grossmann, K; Schüpbach-Regula, G; Thür, B

2014-09-11

343

Improvement of the Shami goat semen quality by adding bovine serum albumin  

Directory of Open Access Journals (Sweden)

Full Text Available The present study was aimed to improve the quality of Shami goat semen diluted with Tris diluent by adding bovine serum albumin. In the current study, six male goats were used. Semen was collected using artificial vagina of one ejaculate per week of every male included in this study. This study was performed during the breeding season from 1 \\ 10 \\ 2012 to 1 \\ 12 \\ 2012. In this study, two semen diluents were use first; Tris- fructose- egg yolk 2.5% and second Tris - fructose - 2.5% egg yolk with 1% of bovine serum albumin. Diluted semen samples were cooled gradually and stored at 5 ° C. Cooled diluted semen samples were examined every 24 h of storage to 144 h. These tests includes the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility using a computer-aided sperm analysis. These results showed that the addition of bovine serum albumin with egg yolk to semen of male goats led to improved qualities of semen significantly (P<0.05 including the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility. It could be concluded from the results of the current study, the possibility of storing goat semen for more than six days with alive sperm of more than 50% and the percentage of the progressive motility of more than 40% when adding bovine albumin serum to dilute goat semen at 1% level and this result has not reached by any previous study.

O.I. Azawi

2014-06-01

344

The reference values for semen parameters of 1213 fertile men in Guangdong Province in China.  

Science.gov (United States)

Semen samples were collected from 1213 fertile men whose partners had a time-to-pregnancy (TTP) ?12 months in Guangdong Province in Southern China, and semen parameters including semen volume, sperm concentration, total counts, motility, and morphology were evaluated according to the World Health Organization (WHO) 2010 guideline. All semen parameters analyzed were normal in ~62.2% of the total samples, whereas ~37.8% showed at least one of the semen parameters below normal threshold values. The fifth centiles (with 95% confidence intervals) were 1.3 (1.2-1.5) ml for semen volume, 20 × 10 6 (18×10 6 -20×10 6 ) ml-1 for sperm concentration, 40 × 10 6 (38×10 6 -44×10 6 ) per ejaculate for total sperm counts, 48% (47%-53%) for vitality, 39% (36%-43%) for total motility, 25% (23%-27%) for sperm progressive motility, 5.0% (4%-5%) for normal morphology. The pH values ranged from 7.2 to 8.0 with the mean ± standard deviation at 7.32 ± 0.17. No effects of age and body mass index were found on semen parameters. Occupation, smoking and alcohol abuse, varicocele appeared to decrease semen quality. Sperm concentration, but not sperm morphology, is positively correlated with TTP, whereas vitality is negatively correlated with TTP. Our study provides the latest reference values for the semen parameters of Chinese fertile men in Guangdong Province, which are close to those described in the new WHO guidelines (5 th Edition). PMID:25432502

Tang, Yun-Ge; Tang, Li-Xin; Wang, Qi-Ling; Song, Ge; Jiang, Yan-Jia; Deng, Shun-Mei; Jiang, Fang; Qin, Wei-Bing

2015-01-01

345

Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men  

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Full Text Available Abstract Background Genital ureaplasmas (Ureaplasma urealyticum and Ureaplasma parvum and mycoplasmas (Mycoplasma genitalium and Mycoplasma hominis are potentially pathogenic species playing an etiologic role in both genital infections and male infertility. Reports are, however, controversial regarding the effects of these microorganisms infections in the sperm seminological variables. This study aimed at determining the frequency of genital ureplasmas and mycoplasmas in semen specimens collected from infertile men, and at comparing the seminological variables of semen from infected and non-infected men with these microorganisms. Methods A total of 120 semen samples collected from infertile men were investigated. Semen specimens were examined by in-house PCR-microtiter plate hybridization assay for the presence of genital ureaplasmas and mycoplasmas DNA. Semen analysis was assessed according to the guidelines of the World Health Organization. Standard parametric techniques (t-tests and nonparametric techniques (Wilcoxon tests were used for statistical analysis. Results The frequency of genital ureaplasmas and mycoplasmas detected in semen samples of infertile men was respectively 19.2% (23/120 and 15.8% (19/120. The frequency of Ureaplasma urealyticum (15% was higher than that of Mycoplasma hominis (10.8%, Ureaplasma parvum (4.2% and Mycoplasma genitalium (5%. Mixed species of mycoplasmas and ureaplasmas were detected in 6.7% of semen samples. Comparison of the parameters of the standard semen analysis between the male partners of the infertile couples with and without genital ureaplasmas and mycoplasmas infection showed that the presence of Mycoplasma hominis DNA in semen samples is associated with low sperm concentration (p = 0.007 and abnormal sperm morphology (p = 0.03 and a negative correlation between sperm concentration and the detection of Mycoplasma genitalium in semen samples of infertile men (p = 0.05. The mean values of seminal volume, pH, vitality, motility and leukocyte count were not significantly related either to the detection of genital mycoplasmas DNA or to the detection of ureaplasmas DNA in semen specimens. Conclusion Our results demonstrate that genital mycoplasmas and ureaplasmas seem to be widespread among the male partners of infertile couples in Tunisia. Genital mycoplasmas infections of the male genital tract could negatively influence semen quality. Our results also indicate that PCR-microtiter plate hybridization assay method provides a rapid and effective technique to detect human genital mycoplasmas and ureaplasmas which is useful for etiological and epidemiological studies of these pathogens.

Rebai Tarek

2007-11-01

346

Effects of Alpha Lipoic Acids on Cattle Sperm Kinetics Using Computer Assisted Semen Analysis  

OpenAIRE

This study investigated the protective effect of Alpha Lipoic Acid (ALA) on animal sperm quality using Computer Assisted Semen Analysis (CASA). Fresh semen sample collected from adult Limousin bulls. The experiment involved five test groups and a control. Alpha lipoic acid with different concentrations (0.1, 0.05, 0,025, 0.0125 and 0.00625 mmol mL-1) incubated into semen from all test groups. They were cryopreserved and thawed after 1 h. CASA analysis prior to cryopresevation confirmed the ba...

Amat Aswadi Abd Karim; Mohd Iswadi Ismail; Nor Azlina Zakaria; Siti Fatimah Ibrahim; Khairul Osman; Zawawi Ismail

2012-01-01

347

Sperm Chromatin Structure, Semen Quality and Lead in Blood and Seminal Fluid among Infertility Men  

OpenAIRE

Background: Exposures to lead above the threshold value of 50–60 ?g/dL have been linked to diminished semen quality parameters. Worldwide, the lead exposure has been diminished during the last years. Therefore, it has become of a great concern to examine the effects of lead exposures on semen quality at low levels of exposure.Objective: To evaluate the effect of low level (<20 µg/dL) blood lead on semen quality and sperm chromatin structure.Methods: A cross-sectional study w...

Mansour, M.; Mandour, R.; Gouida, M.; Nj, Awadalla; El-helaly, M.

2010-01-01

348

Improvement of the Shami goat semen quality by adding bovine serum albumin  

OpenAIRE

The present study was aimed to improve the quality of Shami goat semen diluted with Tris diluent by adding bovine serum albumin. In the current study, six male goats were used. Semen was collected using artificial vagina of one ejaculate per week of every male included in this study. This study was performed during the breeding season from 1 \\ 10 \\ 2012 to 1 \\ 12 \\ 2012. In this study, two semen diluents were use first; Tris- fructose- egg yolk 2.5% and second Tris - fructose - 2.5% egg yolk ...

Azawi, O. I.; Salman, O. J.

2014-01-01

349

Evaluation of Physical Semen Characteristics of Male Rabbits Exposed to Different Climatic Conditions and Lighting Regimes Using Nuclear Techniques  

International Nuclear Information System (INIS)

The number of 20 mature males New Zealand White (NZW) rabbits, in the first production year was used in the present research. The study included two periods; each was of 2 months. The first period was under mild conditions (18.0 degree C) while the second one was during hot conditions (35.0 degree C). In each period, 10 males with the same age and average live body weight were used. Animals within each period were divided randomly into two equal groups, with nearly equal body weights. One of the two groups exposed to natural day light (NDL) which was 10:50 L: 13:10 D in winter and 13:40 L: 10:20 D in summer and was considered as photo period control and the other group was exposed to photo period treatment (Artificial photo period, AP). The treatment group was exposed to artificial long photo period (13:40 L: 10:20 D) during winter and artificial short photo period (10:50 L: 13:10 D) during hot conditions. In seminal plasma, T4, T3 and testosterone hormonal levels were significantly lower in heat stressed rabbits than those reared under mild conditions. In contrast, the hot condition was accompanied by significant increases in cortisol level. T3 and cortisol affected significantly while T4 and testosterone levels were not affected significantly due to change in period of lighting. Concerning physical semen characteristics i.e. ejaculate volume, sperm motility, sperm cell concentration, total sperm output and number of motile sperms per ejaculate were significantly lower under heat stress than under mild conditions. In contrast, hot conditions were accompanied by a significant increase in each of reaction time, dead sperm %, sperm abnormalities % and acrosomal abnormalities %. Exposure of male rabbits during winter to long lighting as compared to NDL caused significant increase in T3 (1.4 vs. 1.3 ng/ml), testosterone (3.2 vs. 2.8 ng/ml) and cortisol (1.8 vs. 1.5 ng/ml) levels as well as significant decline in semen quality, i.e., ejaculate volume (70 vs. 60 x 10-2 ml), sperm motility (76.8 vs. 70.8%), total number sperm-cell output per ejaculate (287.00 vs. 240.00 x106 ) and number of motility sperm output per ejaculate (220.42 vs. 169.92 x106 ). Exposure of male rabbits during summer to short lighting as compared to NDL caused significant increase in T3 (1.10 vs.0.90 ng/ml) and cortisol (2.8 vs. 2.3 ng/ml) in seminal plasma as well as significant decrease in sperm motility (64.8 vs. 60.8%) and significant increase in reaction time (11.6 vs. 12.8 seconds), ejaculate volume (50 vs. 58 x 10-2 ml) and total number sperm-cell output per ejaculate (190.00 vs. 208.80 x 106 ). Finally, correlations between physical semen characteristics and seminal plasma hormonal levels were carried out to evaluate the rabbit bucks semen using nuclear technique

350

Estandarización del manejo y la criopreservación de semen de hembras masculinizadas de trucha arco iris (Oncorhynchus mykiss Padronizar a gestão ea criopreservação de sêmen de fêmeas de truta arco-íris (Oncorhynchus mykiss sob masculinização Standardization of handling and freezing sperm from masculinized females of rainbow trout (Oncorhynchus mykiss  

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Full Text Available La demanda de líneas monosexo hembras en la producción de trucha ha incrementado significativamente en los últimos años, por lo que se han desarrollado tecnologías para estandarizar este proceso como el uso de semen de hembras genéticas sometidas a reversión sexual. El objetivo de la presente investigación fue estandarizar la maduración in vitro y la criopreservación de semen de hembras masculinizadas (neomachos XX de trucha arco iris (Oncorhynchus mykiss como estrategia para producir descendencias 100% hembras de reproductores colombianos. Para la obtención del semen los neomachos fueron sacrificados y el semen recuperado fue sometido a proceso de maduración con plasma seminal de machos normales o plasma seminal artificial. Para la criopreservación del semen se probaron los crioprotectores dimetilsulfóxido 10% y metanol 10%. En el experimento se evaluron la movilidad post maduración y post descongelación y la fertilidad del semen. El proceso de maduración tuvo un efecto significativo sobre el porcentaje de movilidad (pA procura de linhas monosexo fêmeas na produção de trutas tem aumentado significativamente nos últimos anos, de modo tecnologias foram desenvolvidas com a finalidade de padronizar este processo como o uso do esperma de genética feminina submetido a reversão sexual. O objectivo do presente inquérito foi para uniformizar a maturação in vitro e criopreservação de sêmen masculinização de fêmeas (neomachos XX trutas arco-íris (Oncorhynchus mykiss como uma estratégia para produzir descendentes de 100% do sexo feminino dos jogadores colombianos. Para a obtenção do esperma neomachos foram mortas e sêmen foi recuperado submetida a maturação processo normal de plasma seminal plasma seminal masculina ou artificiais. Para a criopreservação de sêmen foi testado crioprotectores dimethylsulphoxide 10% e 10% de metanol. O experimento foi evaluron mobilidade pós maturação e pós descongelamento e fertilidade do sêmen. O processo de maturação teve um efeito significativo sobre a porcentagem de mobilidade (pThe demand of monosex female stocks in production of trout has significantly increased during the past years, which has led to develop new technologies to standardize this process. The usage of semen of genetic females submitted to sexual reversion is a good choice. The objective of this research was to develop a methodology to mature in vitro and cryopreserved semen of sex-reversed rainbow trout (Oncorhynchus mykiss females as strategy to produce lineage 100% Colombian trout female. The semen was directly obtained from the gonads after its surgical extraction of the slaughtered individuals, later it was submitted to maturation process implementing seminal plasma of normal males and artificial plasma. The semen was cryopreserved in two extender dimetyhyl sulfoxide 10% and methanol 10%. Postmaturation, postcriopreservation movility and sperm fertility were evaluated. Maturation process had a significative effect on movility, the highest movility was obtained with artificial seminal plasma (55 ± 10.4 %. Highest post criopreservation movility (29.9 ± 13.3% and highest fertility rates (26.33 ± 7.53 % were obtained with dimetyhyl sulfoxide 10%.

James J Betancur L

2008-09-01

351

Estandarización del manejo y la criopreservación de semen de hembras masculinizadas de trucha arco iris (Oncorhynchus mykiss) / Standardization of handling and freezing sperm from masculinized females of rainbow trout (Oncorhynchus mykiss) / Padronizar a gestão ea criopreservação de sêmen de fêmeas de truta arco-íris (Oncorhynchus mykiss) sob masculinização  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in portuguese A procura de linhas monosexo fêmeas na produção de trutas tem aumentado significativamente nos últimos anos, de modo tecnologias foram desenvolvidas com a finalidade de padronizar este processo como o uso do esperma de genética feminina submetido a reversão sexual. O objectivo do presente inquérito [...] foi para uniformizar a maturação in vitro e criopreservação de sêmen masculinização de fêmeas (neomachos XX) trutas arco-íris (Oncorhynchus mykiss) como uma estratégia para produzir descendentes de 100% do sexo feminino dos jogadores colombianos. Para a obtenção do esperma neomachos foram mortas e sêmen foi recuperado submetida a maturação processo normal de plasma seminal plasma seminal masculina ou artificiais. Para a criopreservação de sêmen foi testado crioprotectores dimethylsulphoxide 10% e 10% de metanol. O experimento foi evaluron mobilidade pós maturação e pós descongelamento e fertilidade do sêmen. O processo de maturação teve um efeito significativo sobre a porcentagem de mobilidade (p Abstract in spanish La demanda de líneas monosexo hembras en la producción de trucha ha incrementado significativamente en los últimos años, por lo que se han desarrollado tecnologías para estandarizar este proceso como el uso de semen de hembras genéticas sometidas a reversión sexual. El objetivo de la presente invest [...] igación fue estandarizar la maduración in vitro y la criopreservación de semen de hembras masculinizadas (neomachos XX) de trucha arco iris (Oncorhynchus mykiss) como estrategia para producir descendencias 100% hembras de reproductores colombianos. Para la obtención del semen los neomachos fueron sacrificados y el semen recuperado fue sometido a proceso de maduración con plasma seminal de machos normales o plasma seminal artificial. Para la criopreservación del semen se probaron los crioprotectores dimetilsulfóxido 10% y metanol 10%. En el experimento se evaluron la movilidad post maduración y post descongelación y la fertilidad del semen. El proceso de maduración tuvo un efecto significativo sobre el porcentaje de movilidad (p Abstract in english The demand of monosex female stocks in production of trout has significantly increased during the past years, which has led to develop new technologies to standardize this process. The usage of semen of genetic females submitted to sexual reversion is a good choice. The objective of this research wa [...] s to develop a methodology to mature in vitro and cryopreserved semen of sex-reversed rainbow trout (Oncorhynchus mykiss) females as strategy to produce lineage 100% Colombian trout female. The semen was directly obtained from the gonads after its surgical extraction of the slaughtered individuals, later it was submitted to maturation process implementing seminal plasma of normal males and artificial plasma. The semen was cryopreserved in two extender dimetyhyl sulfoxide 10% and methanol 10%. Postmaturation, postcriopreservation movility and sperm fertility were evaluated. Maturation process had a significative effect on movility, the highest movility was obtained with artificial seminal plasma (55 ± 10.4 %). Highest post criopreservation movility (29.9 ± 13.3%) and highest fertility rates (26.33 ± 7.53 %) were obtained with dimetyhyl sulfoxide 10%.

James J, Betancur L; Andrés F, Montoya; Tatiana, Mira; Francy A, Rojas; Martha, Olivera Ángel.

2008-09-01

352

Effect of Substitution of Buffalo Bull Seminal Plasma with That of Cow Bull on Liveability and Conception Rate  

OpenAIRE

The present project was designed to study the effect of substitution of buffalo bull seminal plasma with that of cow bull on liveability and fertility of buffalo bull spermatozoa. After collection, semen was divided into three fractions. a) Half of the seminal plasma of buffalo bull was substituted with equal amount of cow bull seminal plasma, b) The seminal plasma of buffalo bull was completely substituted with that of cow bull, c) No substitution was made (control group). The liveability (h...

Lodhi, L. A.; Qureshi, Z. I.; Chohan, F. R.; Iqbal, J.; Ahmad, I.

1998-01-01

353

Correlation between Sperm Parameters and Protein Expression of Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study.  

Science.gov (United States)

Background. Semen analysis is the cornerstone in the evaluation of male (in)fertility. However, there are men with normal semen tests but with impaired fertilizing ability, as well as fertile men with poor sperm characteristics. Thus, there is rising interest to find novel parameters that will help to predict and define the functional capacity of spermatozoa. Methods. We examined whether there is a correlation between semen parameters (count, progressive motility, and morphology) and protein expression/activity of antioxidative defense enzymes in seminal plasma from 10 normospermic subjects. Results. Sperm progressive motility was in positive correlation with seminal plasma protein expression of both superoxide dismutase (SOD) isoforms (MnSOD and CuZnSOD) and catalase. Also, positive correlation was observed between sperm count and MnSOD protein expression, as well as between sperm morphology and protein expression of catalase in seminal plasma. In contrast, protein expression of glutathione peroxidase was not in correlation with any sperm parameter, while its activity negatively correlated with sperm morphology and motility. Conclusions. These data suggest that evaluation of protein expression of antioxidative defense enzymes in seminal plasma might be of importance in the evaluation of male fertility status and that could be used as an additional biomarker along with classic semen analysis in assessment of semen quality. PMID:25691806

Macanovic, Biljana; Vucetic, Milica; Jankovic, Aleksandra; Stancic, Ana; Buzadzic, Biljana; Garalejic, Eliana; Korac, Aleksandra; Korac, Bato; Otasevic, Vesna

2015-01-01

354

Criopreservação do sêmen ovino em meio diluente à base de água de coco em pó (ACP-102c) / Cryopreservation of ram semen in powdered coconut water (ACP-102c) based extender  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O objetivo deste trabalho foi avaliar o diluente ACP-102c na criopreservação do sêmen ovino em comparação com o diluidor tris-glicose-gema (TRIS) e o sêmen fresco. Foram coletados 48 ejaculados de quatro ovinos, sendo tomadas duas alíquotas por ejaculado para diluição e criopreservação em ACP-102c o [...] u TRIS e uma terceira alíquota utilizada para análise do sêmen fresco. O sêmen fresco e o criopreservado em ambos os diluidores foram avaliados para viabilidade, integridade de membrana plasmática e acrossomal, teste hiposmótico, fragmentação do DNA e de motilidade espermática. Após descongelamento, ambos os diluidores não diferiram para viabilidade espermática, integridade de membrana plasmática e acrossomal, fragmentação de DNA e nas variáveis quantitativas e qualitativas de velocidade espermática, mas diferiram no teste hiposmótico, motilidade total e progressiva e amplitude lateral da cabeça, bem como em todas as variáveis de motilidade avaliadas, exceto linearidade e progressividade, após duas horas de incubação à 37 ºC. Houve variabilidade entre reprodutores na motilidade total e progressiva do sêmen criopreservado em ACP-102c após descongelamento. O diluidor ACP-102c conferiu menor proteção aos espermatozoides ovinos contra danos do congelamento quando comparado ao TRIS, mas o aprimoramento de sua formulação e protocolos mais adequados de congelação poderão torná-lo uma alternativa na congelação do sêmen ovino. Abstract in english The aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP- [...] 102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 ºC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.

José Maurício Maciel, Cavalcante; Oscar Oliveira, Brasil; Cristiane Clemente de Mello, Salgueiro; Carminda Sandra Brito, Salmito-Vanderley; José Ferreira, Nunes.

2014-09-01

355

Withania somnifera improves semen quality by combating oxidative stress and cell death and improving essential metal concentrations.  

Science.gov (United States)

This study investigated the effect of a 3-month treatment with Withania somnifera on apoptosis and intracellular reactive oxygen species (ROS) concentration of spermatozoa and the metal ions copper, zinc, iron and gold in seminal plasma from infertile men (normozoospermic, n=25; oligozoospermic, n=25; and asthenozoospermic, n=25). The apoptotic and necrotic cell distribution were analysed by annexin-V binding and propidium iodide uptake using flow cytometry. ROS generation was measured by fluorescence intensity and metal ions were analysed by atomic absorption spectrophotometry. The results demonstrated that, prior to treatment, sperm apoptosis and intracellular ROS concentrations were significantly higher in all groups of infertile men compared with controls (Pzinc, iron, and gold in seminal plasma were measured. The apoptotic and necrotic cell distribution were analysed by annexin-V binding and propidium iodide uptake using flow cytometry. ROS generation was measured by fluorescence intensity and metal ions were analysed by atomic absorption spectrophotometry. The results demonstrated that prior, to treatment, apoptosis and intracellular ROS concentrations were significantly higher in all groups of infertile men compared with controls. Similarly, the concentrations of the essential metal ions Cu(2+), Zn(2+), Fe(2+) and Au(2+) in seminal plasma were lower. Treatment with W. somnifera significantly reduced apoptosis and ROS concentrations and improved metal ion concentrations in infertile subjects. It is concluded that W. somnifera improves semen quality by reducing oxidative stress and cell death and improving essential metal ion concentrations. PMID:21388887

Shukla, Kamla Kant; Mahdi, Abbas Ali; Mishra, Vivek; Rajender, Singh; Sankhwar, Satya Narain; Patel, Devender; Das, Mukul

2011-05-01

356

Reproductive toxicity of chromium in adult bonnet monkeys (Macaca radiata Geoffrey). Reversible oxidative stress in the semen  

International Nuclear Information System (INIS)

The present study was designed to test the hypothesis that oxidative stress mediates chromium-induced reproductive toxicity. Monthly semen samples were collected from adult monkeys (Macaca radiata), which were exposed to varying doses (50, 100, 200 and 400 ppm) of chromium (as potassium dichromate) for 6 months through drinking water. Chromium treatment decreased sperm count, sperm forward motility and the specific activities of antioxidant enzymes, superoxide dismutase and catalase, and the concentration of reduced glutathione in both seminal plasma and sperm in a dose- and duration-dependent manner. On the other hand, the quantum of hydrogen peroxide in the seminal plasma/sperm from monkeys exposed to chromium increased with increasing dose and duration of chromium exposure. All these changes were reversed after 6 months of chromium-free exposure period. Simultaneous supplementation of vitamin C (0.5 g/L; 1.0 g/L; 2.0 g/L) prevented the development of chromium-induced oxidative stress. Data support the hypothesis and show that chronic chromium exposure induces a reversible oxidative stress in the seminal plasma and sperm by creating an imbalance between reactive oxygen species and antioxidant system, leading to sperm death and reduced motility of live sperm

357

Change in semen quality and sperm chromatin structure following occupational styrene exposure. ASCLEPIOS  

DEFF Research Database (Denmark)

Organic solvents have been suspected to exert detrimental effects on human spermiogenesis. Styrene, which is both mutagenic and neurotoxic, was selected as a suitable organic solvent for further assessment of a possible effect on semen quality and sperm DNA damage.

Kolstad, Henrik; Bonde, J P

1999-01-01

358

Exposure to perfluorinated compounds and human semen quality in Arctic and European populations  

DEFF Research Database (Denmark)

Perfluorinated compounds (PFCs) have been suspected to adversely affect human reproductive health. The aim of this study was to investigate the associations between PFC exposure and male semen quality.

Toft, G; Jönsson, B A G

2012-01-01

359

Exposure to perfluorinated compounds and human semen quality in arctic and European populations  

DEFF Research Database (Denmark)

Perfluorinated compounds (PFCs) have been suspected to adversely affect human reproductive health. The aim of this study was to investigate the associations between PFC exposure and male semen quality.

Toft, Gunnar; Jönsson, B A G

2012-01-01

360

The Effect of Shelter on Semen Quality of “Peranakan Ettawa” Goat  

Directory of Open Access Journals (Sweden)

Full Text Available The experiment was conducted to study the effect of shelter on semen quality of Peranakan Ettawa (PE Goats Eight PE goats were allocated into cross over design. Four PE goats were placed under no shelter (09.00-14.30 and another one was placed under shelter. The results of this research showed that semen volume, sperm motility, sperm concentration, and live sperm percentage of PE goat under shelter were higher (P<0.01 than those of PE goat under no shelter. On the other hand, sperm abnormality of PE goat under shelter was lower (P<0.01 than that of PE goat under no shelter. It was concluded that the use of shelter could improve semen quality. (Animal Production 9(2: 73-78 (2007 Key Words : Shelter, semen, goat

A Qisthon

2007-05-01

361

Is semen a useful diagnostic tool for rare infections of the central nervous system?  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Given that the blood-testis barrier is more permeable than the blood–brain barrier, the use of semen to detect rare parasitic antigens/infections of the CNS in males is hypothesized.

Siddiqui Ruqaiyyah

2012-12-01

362

Effect of Air Space in Storage Vials on Motility of Spermatozoa in Chilled Buck Semen  

Directory of Open Access Journals (Sweden)

Full Text Available This study was conducted in order to find out the effect of air space on the top of glass vial in which semen is stored, on the motility of spermatozoa. 45 samples collected from two bucks over a span of 6 months were used for experiment. Goat milk extender was the diluent used. Two ml each of diluted semen after noting their initial motility was stored in 2 ml and 5 ml vials. Samples were stored at 5°C and motility of spermatozoa noted at 24 and 48 hours. Semen without air space was found to preserve the motility better than semen with air space on 24 and 48 hours of incubation. This could be better attributed to reactive oxygen species production by the spermatozoa, but further investigation is needed in this aspect to confirm it. [Veterinary World 2010; 3(9.000: 421-423

Magnus Paul K and Lali F Anand 1

363

The Effect of Phyto-Lecithin on Preservation and Cryopreservation of Semen: A Review  

Directory of Open Access Journals (Sweden)

Full Text Available Artificial insemination represents one of technologies in livestock reproduction that can be applied to cattle, sheep, goats and other livestock. Application of livestock reproduction technology includes artificial insemination to increase reproductive efficiency. Semen processing is one critical phase in an artificial insemination program. The use of animal origin ingredient for semen extenders, such as egg yolk and milk, presents a risk of microbial contamination, which lead to the search for alternatives. To increase standard of quality, researchers exploits phyto-lesitin for semen extender and the results showed no significant differences in motility, viability, and acrosomal status of spermatozoa with phyto-lesitin extender when compared to tris-egg yolk-containing extenders. (Animal Production 9(1: 49-52 (2007 Key Words : Phyto-Lechitin, preservation, cryopreservation, semen

AS Aku

2007-01-01

364

Semen banking: consideration on viral contamination in the era of new emerging viral infection  

Directory of Open Access Journals (Sweden)

Full Text Available To construct a semen bank, the collection of donated semen has to be done and an important concern is the safety of collected semen. The contamination is a big problem. Basically, the infectious pathogens can exist within donated semen, hence, a good donor screening is very important. Although viruses have an indirect role in sperm quality, but the evidence in banked semen is presently lack. This does not mean that there is no viral contamination but it might imply the inadequate concern on this issue. Contaminated semen usually means poor quality and hazardous to the recipient. The contamination of the virus in banked semen is a common problem in animal semen banking (1. The safety and transmission of each problematic virus is widely studied and well clarified in animal semen banking (2. However, this issue is not widely concerned in human semen banking. For sure, this case is an actual direct contamination and this cannot be detected if there is no specific screening in the banking process. The scenario of important new emerging viral infections will be specifically detailed in this report. West Nile virus is an emerging problematic viral infection that can cause a deadly clinical disorder. Basically, West Nile virus is classified as an arbovirus that is mainly transmitted by mosquito. However, the uncommon modes of transmissions such as transfusion related transmission are reported (3. The contamination of West Nile virus in semen is an important question in andrology. There is no evidence indicating for the presence of West Nile virus in the semen of the patients. However, American Society for Reproductive Medicine/Society for Assisted Reproductive Technology recommended that practitioners defer gamete donors who have confirmed or suspected West Nile virus infections (4. SARS is another deadly emerging viral infection. The new coronavirus infection is transmitted via respiratory route. The serious symptom due to this infection leads to death in almost all cases and brings a great concern to medical scientists around the world. The contamination of SARS in semen is an interesting topic. The possible transmission of SARS virus via germ line is an important question to be investigated in reproductive medicine (5. Luckily, till present, there is no evidence of SARS contamination in semen. Generally, influenza virus is a respiratory virus that causes respiratory tract infection. In the recent few years, an atypical influenza, avian flu, emerged. This infection brought a concern to the medical society. In early of this year, 2009, the newest emerging viral infection caused by a novel influenza virus, swine flu occurred and became pandemic. The topic on the new influenza virus becomes the present hot issue. Focusing on the contamination of classical influenza virus in semen, there are many evidences confirming the existence of virus in semen derived from the infected cases. It is also

Viroj Wiwanitkit

2011-01-01

365

Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen.  

Science.gov (United States)

Naturally occurring fragments of the abundant semen proteins prostatic acid phosphatase (PAP) and semenogelins form amyloid fibrils in vitro. These fibrils boost HIV infection and may play a key role in the spread of the AIDS pandemic. However, the presence of amyloid fibrils in semen remained to be demonstrated. Here, we use state of the art confocal and electron microscopy techniques for direct imaging of amyloid fibrils in human ejaculates. We detect amyloid aggregates in all semen samples and find that they partially consist of PAP fragments, interact with HIV particles and increase viral infectivity. Our results establish semen as a body fluid that naturally contains amyloid fibrils that are exploited by HIV to promote its sexual transmission. PMID:24691351

Usmani, Shariq M; Zirafi, Onofrio; Müller, Janis A; Sandi-Monroy, Nathallie L; Yadav, Jay K; Meier, Christoph; Weil, Tanja; Roan, Nadia R; Greene, Warner C; Walther, Paul; Nilsson, K Peter R; Hammarström, Per; Wetzel, Ronald; Pilcher, Christopher D; Gagsteiger, Friedrich; Fändrich, Marcus; Kirchhoff, Frank; Münch, Jan

2014-01-01

366

The importance of semen analysis in the context of azoospermia  

Scientific Electronic Library Online (English)

Full Text Available Azoospermia is a descriptive term referring to ejaculates that lack spermatozoa without implying a specific underlying cause. The traditional definition of azoospermia is ambiguous, which has ramifications on the diagnostic criteria. This issue is further compounded by the apparent overlap between t [...] he definitions of oligospermia and azoospermia. The reliable diagnosis of the absence of spermatozoa in a semen sample is an important criterion not only for diagnosing male infertility but also for ascertaining the success of a vasectomy and for determining the efficacy of hormonal contraception. There appears to be different levels of rigor in diagnosing azoospermia in different clinical situations, which highlights the conflict between scientific research and clinical practice in defining azoospermia.

Nabil, Aziz.

367

CONTRIBUTIONS ON THE ATTENUATE OF THE CRYOGENICS RESPONSE OF CONSTITUENTS PROTEINS HOMEOSTASIS OF THE SEMEN MATERIAL  

Directory of Open Access Journals (Sweden)

Full Text Available The evidence of cryogenics response of the semen proteins, the influence of BioR administration on homeostasis of constituent gametes proteomics and on the cryobiological indexes of bull semen material was studded. The investigation has been performed on bulls from the Black Spotted breed of Moldavian type, maintained during the investigation in adequate conditions from the point of view of microclimate and fodder. The biopreparation administration have been done daily during 10 days in volume of 0,2 ml/100 kg living mass/day. Structural proteins of gametes posed the resistance given the influence of ultra low temperature (-196°C, content of totals proteins in the bull semen material denote no difference between the value of this parameter in the raw and cry preserved-thawed bull gametes. Both, in the raw and thawed semen cells the most rate occupy the hydrophilic proteins, After semen conservation-thawing process, it was observed a tendency of the diminution of hydrophilic proteins (- 3,35% and an increase of the basophilic proteins (+ 2,78 %. In the raw gametes prevail ?-globulins rate; conservation and thawing process of the semen material was associated by an increase of the albumins rate (+ 34,63% in semen cells; the rate of other three proteomic fractions: ?-, ? - and ?-globulins was decreased given theirs value registered in raw gametes. After the intramuscular administration of BioR preparation during 10 days on the sire bulls have been certified any modification of the studded proteomic fractions rate in thawed bull semen cells; albumins rate was decreased with 30,14%, the ?- globulins rate was increased with 19,28% in the experimental group; the ?- and ?- globulins with 8,5% and 2,36%, respectively, given control group. The BioR has an evident influence on the cryobiological specifics features of spermatozoids, such as the seminal cells mobility, the longevity and the survival absolutly index what are intensely influenced.

VERA GRANACI

2013-12-01

368

Estudio preliminar de colección de semen en oso de anteojos (Tremarctos ornatus)  

Scientific Electronic Library Online (English)

Full Text Available [...] Abstract in english Semen was collected in a Spectacled Bear (Tremarctos ornatus) reared in captivity using the electroejaculation technique. Four series of 6 volt discharges by 15 seconds each plus manual stimulation were carried out. An effective penis erection and small volume of ejaculate was obtained in the last s [...] eries of electrical stimulus. Seminal motility was 50%. Further studies are required to optimize the use of the electroejaculator in order to obtain higher volumes and better semen quality.

Marco, Enciso H; Lizette, Bermúdez L.; Shirley, Evangelista V; Gianmarco, Rojas M.; Wilfredo, Huanca L..

2006-01-01

369

The Effect of Shelter on Semen Quality of “Peranakan Ettawa” Goat  

OpenAIRE

The experiment was conducted to study the effect of shelter on semen quality of Peranakan Ettawa (PE) Goats Eight PE goats were allocated into cross over design. Four PE goats were placed under no shelter (09.00-14.30) and another one was placed under shelter. The results of this research showed that semen volume, sperm motility, sperm concentration, and live sperm percentage of PE goat under shelter were higher (P

Qisthon, A.; Suharyati, S.

2007-01-01

370

Field investigations of bacterial contaminants and their effects on extended porcine semen.  

Science.gov (United States)

Field investigations (n=23) were made over a 3-yr period at North American boar studs and farms in which the primary complaint was sperm agglutination in association with decreased sperm longevity of extended semen, and increased regular returns to estrus and/or vaginal discharges across parity. Microscopic examination of extended semen from these units revealed depressed gross motility (usually 20%). Sample pH was acidic (5.7 to 6.4) in 93% of the submitted samples. Aerobic culture yielded a variety of bacteria from different genera. A single bacterial contaminant was obtained from 66% of the submitted samples (n=37 doses); 34% contained 2 or more different bacterial genera. The most frequently isolated contaminant bacteria from porcine extended semen were Alcaligenes xylosoxydans (n=3), Burkholderia cepacia (n=6), Enterobacter cloacae (n=6), Escherichia coli (n=6), Serratia marcescens (n=5), and Stenotrophomonas [Xanthomonas] maltophilia (n=6); these 6 bacteria accounted for 71% of all contaminated samples, and were spermicidal when re-inoculated and incubated in fresh, high quality extended semen. All contaminant bacteria were found to be resistant to the aminoglycoside gentamicin, a common preservative antibiotic used in commercial porcine semen extenders. Eleven genera were spermicidal in conjunction with an acidic environment, while 2 strains (E. coli, S. maltophilia) were spermicidal without this characteristic acidic environment. Bacteria originated from multiple sources at the stud/farm, and were of animal and nonanimal origin. A minimum contamination technique (MCT) protocol was developed to standardize hygiene and sanitation. This protocol focused on MCT's during boar preparation, semen collection, semen processing and laboratory sanitation. Implementation of the MCT, in addition to specific recommendations in stud management, resulted in the control of bacterial contamination in the extended semen. PMID:10798493

Althouse, G C; Kuster, C E; Clark, S G; Weisiger, R M

2000-03-15

371

Relationship between semen quality and performance of instrumentally inseminated honey bee queens  

OpenAIRE

Techniques to effectively store honey bee semen must meet some minimally acceptable level of spermatozoa survival. To determine this level, honey bee queens were inseminated using various mixes of fresh and freeze-killed semen, and were allowed to lay eggs in small colonies for three weeks. The queens receiving all freeze-killed spermatozoa (0% fresh) had no spermatozoa in their spermathecae, and produced only drone pupae (unfertilized eggs). The proportions of live and dead spermatozoa (dete...

Collins, Anita

2000-01-01

372

Effects of Aerobic and Anaerobic Incubation On the Quality of Ram Semen  

OpenAIRE

Effects of aerobic and anaerobic incubation on the quality of ram semen were investigated. The semen was assessed applying Activity index, Motility % (PLM), Swim-up test, Osmotic Resistance Test (ORT) and Oxygen utilization of the cells. Methylene blue crystals were used as an indicator for oxygen utilization of the cells. Usage of Methylene blue crystals was found useful as an indicator for oxygen utilization. It was observed that the incubation conditions had a significant (p = 0.05) effect...

Samo, M. U.; Axford, R. F. E.; Qureshi, T. A.; Memon, A. A.; Memon, M. M.

2006-01-01

373

Genetic analysis and models for semen production and artificial insemination result in sheep  

OpenAIRE

In sheep, more than 800,000 articial inseminations (AI) are performed each year in France. In order to improve their eciency, French AI centres would like to increase the number of doses produced per ram and the probability of AI success. We analyzed semen production and AI results from six AI centre members of the ANIO (association nationale des centres d'insemination ovine). Our objectives were (1) the identication of the main environmental eects aecting semen production (volume, concentrat...

David, Ingrid

2008-01-01

374

Optimalization of Equilibration and Thawing Methode on Freezing Process of Garut Ram Semen (Ovis aries)  

OpenAIRE

Generally, the problem of semen freezing process is cold shock effect and intracelullar change due to water release which is related to ice crystal formation. One factor to solve the problem is finding out optimal equilibration time and thawing method so there is only a little damage spermatozoa during freezing process. The research has been done to explore the influence of equilibration time and thawing methode on frozen semen quality of garut rams. The results shown that the mea...

Herdis; MR Toelihere; Supriatna, I.; Purwantara, B.; Rts, Adikara

2005-01-01

375

Semen Contains Vitality and Heredity, Not Germs’: Seminal Discourse in the AIDS Era  

OpenAIRE

Perspectives of public health generally ignore culture-bound sexual health concerns, such as semen loss, and primarily attempt to eradicate sexually transmitted infections (STIs), including human immunodeficiency virus (HIV). Like in many other countries, sexual health concerns of men in Bangladesh have also received less attention compared to STIs in the era of AIDS. This paper describes the meanings of non-STI sexual health concerns, particularly semen loss, in the masculinity framework. In...

Khan, Sharful Islam; Hudson-rodd, Nancy; Saggers, Sherry; Bhuiyan, Mahbubul Islam; Bhuiya, Abbas; Karim, Syed Afzalul; Rauyajin, Oratai

2006-01-01

376

Detection of human papillomavirus DNA by PCR in semen from patients with and without penile warts.  

OpenAIRE

OBJECTIVES--To determine the prevalence of urethral HPV infection, as indicated by the presence of HPV DNA in semen, in males with and without penile warts. DESIGN--Prevalence study of HPV types 6/11 and 16 DNA using PCR and Southern blot hybridisation analysis of semen. SETTING--Department of Genitourinary Medicine, Blundell Street Clinic, Leeds General Infirmary and the Assisted Conception Unit (ACU) Kings' College, London. SUBJECTS--Patients attending the Genitourinary Clinic for treatment...

Green, J.; Monteiro, E.; Bolton, V. N.; Sanders, P.; Gibson, P. E.

1991-01-01

377

Steps in the investigation and management of low semen volume in the infertile man  

OpenAIRE

An adequate semen volume of ejaculate fluid is required to transport sperm into the female reproductive tract and allow for fertilization of the oocyte. Thus, seminal fluid volume is an important part of the semen analysis done to investigate male infertility. In this article, we review the anatomy and physiology of ejaculation, the various etiologies of low-volume ejaculation (artifactual, structural, functional). We then present a comprehensive algorithm for the evaluation, diagnosis and tr...

Roberts, Matthew; Jarvi, Keith

2009-01-01

378

Evaluation of the microbial quality of fresh ejaculates of camel (Camelus dromedarius) semen.  

Science.gov (United States)

A series of five factorial arranged experiments were conducted to investigate the effect of different management during semen collection on the microbial quality (bacterial load, type of microbes and frequency of isolation) of dromedaries' semen. Microbial analysis of seventy-nine fresh ejaculates from twenty-two camels showed the presence of nine variant colonies. The most prevalent organisms in the dromedary semen were species of Staphylococcus, Bacillus and Streptococcus. One yeast species was represented among the isolates. The middle aged camels (9-13 years) had significantly (P>0.05) higher mean bacterial loads than young (4-8 years) and old aged (14-18 years) animals. The mean bacterial populations of ejaculates collected by an electro-ejaculator were significantly (P>0.05) higher than those collected by an artificial vagina. Candida spp. was identified in 53.8% of the samples collected by an electro-ejaculator and was not detected in ejaculates collected by an artificial vagina. The mean semen bacterial load detected during the breeding season was significantly (P<0.01) higher than that collected during the non-breeding season. No fungi were isolated from semen samples collected in the non-breeding season. The difference between the mean semen bacterial loads in the first and the second ejaculate was highly significant (P<0.01). The preputial wash significantly (P<0.01) reduced the bacterial load. This study revealed that the microbial contamination of dromedaries' semen is found in different intensities during different management procedures of semen collection. PMID:25132210

Ghoneim, I M; Waheed, M M; Al-Hofofi, A N; Fayez, M M; Al-Eknah, M M; Al-Busadah, K A; Al-Humam, N A

2014-10-01

379

Simple and effective methods of freezing capercaillie (Tetrao urogallus L.) semen.  

Science.gov (United States)

A continuous decline in the number and range of capercaillie (Tetrao urogallus L.) in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryopreservation methods for capercaillie semen are discussed. Semen was collected from seven males kept in the Capercaillie Breeding Centre at Forestry Wis?a in Poland. Within five minutes after collection, ejaculates were diluted with EK diluent, then divided into two parts, and subjected to two freezing procedures: in pellets and in straws. In fresh semen, ejaculate clearness, viscosity, color and volume, as well as sperm concentration, motility and morphology, were evaluated, while in frozen-thawed semen only motility and morphology of sperm were determined. Fertilizing ability of thawed semen was examined for samples frozen in straws. Significant (Psperm concentration was 632.5 x 10? mL?¹ (178.8-1257.1); percentage of live normal cells varied from 39.2 to 70.3% (58.7% on an average); percentage of motile cells ranged from 76.0 to 85.7%) and motility parameters were male dependent, as well. Both cryopreservation methods had a negative effect on morphology and motility of frozen-thawed semen; however, the straw method yielded 60.7% and the pellet method 42.5% of live cells in total in thawed semen (Psperm and can be used to create a gene bank of capercaillie semen. PMID:25615640

Kowalczyk, Artur; ?ukaszewicz, Ewa

2015-01-01

380

Possible negative effects of inbreeding on semen quality in Shetland pony stallions  

OpenAIRE

Inbreeding is widely believed to negatively affect reproductive performance. Indeed, in some species, high levels of inbreeding are thought to be the major cause of poor semen quality. It is, however, not clear whether inbreeding affects fertility in horses. In this study, the relationship between inbreeding and semen quality was examined in 285 immature Shetland pony stallions submitted for breeding soundness examination in March-April of the years 1992-1997. The majority of stallions examin...

Eldik, P.; Waaij, E. H.; Ducro, B. J.; Kooper, A. W.; Stout, T. A. E.; Colenbrander, B.

2006-01-01