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Sample records for saos-2 cell calcified

  1. Tissue transglutaminase (TG2 activity regulates osteoblast differentiation and mineralization in the SAOS-2 cell line

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    Xiaoxue Yin

    2012-08-01

    Full Text Available Tissue transglutaminase (type II, TG2 has long been postulated to directly promote skeletal matrix calcification and play an important role in ossification. However, limited information is available on the expression, function and modulating mechanism of TG2 during osteoblast differentiation and mineralization. To address these issues, we cultured the well-established human osteosarcoma cell line SAOS-2 with osteo-inductive conditioned medium and set up three time points (culture days 4, 7, and 14 to represent different stages of SAOS-2 differentiation. Osteoblast markers, mineralization, as well as TG2 expression and activity, were then assayed in each stage. Furthermore, we inhibited TG activity with cystamine and then checked SAOS-2 differentiation and mineralization in each stage. The results showed that during the progression of osteoblast differentiation SAOS-2 cells presented significantly high levels of osteocalcin (OC mRNA, bone morphogenetic protein-2 (BMP-2 and collagen I, significantly high alkaline phosphatase (ALP activity, and the increased formation of calcified matrix. With the same tendency, TG2 expression and activity were up-regulated. Furthermore, inhibition of TG activity resulted in a significant decrease of OC, collagen I, and BMP-2 mRNA and of ALP activity and mineralization. This study demonstrated that TG2 is involved in osteoblast differentiation and may play a role in the initiation and regulation of the mineralization processes. Moreover, the modulating effects of TG2 on osteoblasts may be related to BMP-2.

  2. Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes

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    Matsuoka, Makoto; Akasaka, Tsukasa; Totsuka, Yasunori; Watari, Fumio

    2010-01-01

    In recent years, carbon nanotubes (CNTs) have been considered potential biomedical materials because of their unique character. The aim of this study was to investigate the response of a human osteoblast-like cell line - Saos-2 - on single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs). The surface of a culture dish was coated with CNTs, and Saos-2 cells were cultured for three days. Cell morphology, viability, alkaline phosphatase (ALP) activity, adhesion, and vinculin expression were evaluated. The result showed high cell viability and strong adhesion to MWCNTs. Saos-2 cultured on MWCNTs exhibited vinculin expression throughout the cell body, while the cells attached to SWCNTs and glass were mostly limited to their periphery. Our results suggest that CNT coatings promote cell activity and adhesiveness. These findings indicate that MWCNTs could be used as surface coating materials to promote cell adhesion.

  3. Phenotypic instability of Saos-2 cells in long-term culture

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    Hausser, Heinz-Juergen; Brenner, Rolf E.

    2005-01-01

    The human osteosarcoma cell line Saos-2 is widely used as a model system for human osteoblastic cells, though its phenotypic stability has not been ascertained. We therefore propagated these cells over 100 passages and compared relevant phenotypic properties. In general, higher passage cells exhibited higher proliferation rates and lower specific alkaline phosphatase activities, though mineralization was significantly more pronounced in cultures of late passage cells. Whereas expression of most genes investigated did not vary profoundly, some genes exhibited remarkable differences. Decorin, for instance, that has been discussed as a regulator of proliferation and mineralization, is strongly expressed only in early passage cells, and two receptors for pleiotrophin and midkine exhibited an almost mutually exclusive expression pattern in early and late passage cells, respectively. Our observations indicate that special care is required when results obtained with Saos-2 cells with different culture history are to be compared

  4. SAOS-2 osteosarcoma cells bind fibroblasts via ICAM-1 and this is increased by tumour necrosis factor-α.

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    David, Manu S; Kelly, Elizabeth; Cheung, Ivan; Xaymardan, Munira; Moore, Malcolm A S; Zoellner, Hans

    2014-01-01

    We recently reported exchange of membrane and cytoplasmic markers between SAOS-2 osteosarcoma cells and human gingival fibroblasts (h-GF) without comparable exchange of nuclear markers, while similar h-GF exchange was seen for melanoma and ovarian carcinoma cells. This process of "cellular sipping" changes phenotype such that cells sharing markers of both SAOS-2 and h-GF have morphology intermediate to that of either cell population cultured alone, evidencing increased tumour cell diversity without genetic change. TNF-α increases cellular sipping between h-GF and SAOS-2, and we here study binding of SAOS-2 to TNF-α treated h-GF to determine if increased cellular sipping can be accounted for by cytokine stimulated SAOS-2 binding. More SAOS-2 bound h-GF pe-seeded wells than culture plastic alone (pcells migrating across different microenvironments can influence subsequent interactions with fibroblasts. Since cytokine stimulated binding was comparable in magnitude to earlier reported TNF-α stimulated cellular sipping, we conclude that TNF-α stimulated cellular sipping likely reflects increased SAOS-2 binding as opposed to enhanced exchange mechanisms.

  5. SAOS-2 osteosarcoma cells bind fibroblasts via ICAM-1 and this is increased by tumour necrosis factor-α.

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    Manu S David

    Full Text Available We recently reported exchange of membrane and cytoplasmic markers between SAOS-2 osteosarcoma cells and human gingival fibroblasts (h-GF without comparable exchange of nuclear markers, while similar h-GF exchange was seen for melanoma and ovarian carcinoma cells. This process of "cellular sipping" changes phenotype such that cells sharing markers of both SAOS-2 and h-GF have morphology intermediate to that of either cell population cultured alone, evidencing increased tumour cell diversity without genetic change. TNF-α increases cellular sipping between h-GF and SAOS-2, and we here study binding of SAOS-2 to TNF-α treated h-GF to determine if increased cellular sipping can be accounted for by cytokine stimulated SAOS-2 binding. More SAOS-2 bound h-GF pe-seeded wells than culture plastic alone (p<0.001, and this was increased by h-GF pre-treatment with TNF-α (p<0.001. TNF-α stimulated binding was dose dependent and maximal at 1.16 nM (p<0.05 with no activity below 0.006 nM. SAOS-2 binding to h-GF was independent of serum, while the lipopolysaccharide antagonist Polymyxin B did not affect results, and TNF-α activity was lost on boiling. h-GF binding of SAOS-2 started to increase after 30min TNF-α stimulation and was maximal by 1.5 hr pre-treatment (p<0.001. h-GF retained maximal binding up to 6 hrs after TNF-α stimulation, but this was lost by 18 hrs (p<0.001. FACS analysis demonstrated increased ICAM-1 consistent with the time course of SAOS-2 binding, while antibody against ICAM-1 inhibited SAOS-2 adhesion (p<0.04. Pre-treating SAOS-2 with TNF-α reduced h-GF binding to background levels (p<0.003, and this opposite effect to h-GF cytokine stimulation suggests that the history of cytokine exposure of malignant cells migrating across different microenvironments can influence subsequent interactions with fibroblasts. Since cytokine stimulated binding was comparable in magnitude to earlier reported TNF-α stimulated cellular sipping, we

  6. Aortic calcified particles modulate valvular endothelial and interstitial cells.

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    van Engeland, Nicole C A; Bertazzo, Sergio; Sarathchandra, Padmini; McCormack, Ann; Bouten, Carlijn V C; Yacoub, Magdi H; Chester, Adrian H; Latif, Najma

    Normal and calcified human valve cusps, coronary arteries, and aortae harbor spherical calcium phosphate microparticles of identical composition and crystallinity, and their role remains unknown. The objective was to examine the direct effects of isolated calcified particles on human valvular cells. Calcified particles were isolated from healthy and diseased aortae, characterized, quantitated, and applied to valvular endothelial cells (VECs) and interstitial cells (VICs). Cell differentiation, viability, and proliferation were analyzed. Particles were heterogeneous, differing in size and shape, and were crystallized as calcium phosphate. Diseased donors had significantly more calcified particles compared to healthy donors (Pinnocent bystanders but induce a phenotypical and pathological change of VECs and VICs characteristic of activated and pathological cells. Therapy tailored to reduce these calcified particles should be investigated. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Effect of bioglass on growth and biomineralization of SaOS-2 cells in hydrogel after 3D cell bioprinting.

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    Xiaohong Wang

    Full Text Available We investigated the effect of bioglass (bioactive glass on growth and mineralization of bone-related SaOS-2 cells, encapsulated into a printable and biodegradable alginate/gelatine hydrogel. The hydrogel was supplemented either with polyphosphate (polyP, administered as polyP • Ca2+-complex, or silica, or as biosilica that had been enzymatically prepared from ortho-silicate by silicatein. These hydrogels, together with SaOS-2 cells, were bioprinted to computer-designed scaffolds. The results revealed that bioglass (nanoparticles, with a size of 55 nm and a molar ratio of SiO2 : CaO : P2O5 of 55 : 40 : 5, did not affect the growth of the encapsulated cells. If silica, biosilica, or polyP • Ca2+-complex is co-added to the cell-containing alginate/gelatin hydrogel the growth behavior of the cells is not changed. Addition of 5 mg/ml of bioglass particles to this hydrogel significantly enhanced the potency of the entrapped SaOS-2 cells to mineralize. If compared with the extent of the cells to form mineral deposits in the absence of bioglass, the cells exposed to bioglass together with 100 µmoles/L polyP • Ca2+-complex increased their mineralization activity from 2.1- to 3.9-fold, or with 50 µmoles/L silica from 1.8- to 2.9-fold, or with 50 µmoles/L biosilica from 2.7- to 4.8-fold or with the two components together (100 µmoles/L polyP • Ca2+-complex and 50 µmoles/L biosilica from 4.1- to 6.8-fold. Element analysis by EDX spectrometry of the mineral nodules formed by SaOS-2 revealed an accumulation of O, P, Ca and C, indicating that the mineral deposits contain, besides Ca-phosphate also Ca-carbonate. The results show that bioglass added to alginate/gelatin hydrogel increases the proliferation and mineralization of bioprinted SaOS-2 cells. We conclude that the development of cell-containing scaffolds consisting of a bioprintable, solid and cell-compatible inner matrix surrounded by a printable hard and flexible outer matrix containing

  8. SaOS-2 cell response to macro-porous boron-incorporated TiO{sub 2} coating prepared by micro-arc oxidation on titanium

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    Huang, Qianli [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Elkhooly, Tarek A. [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Department of Ceramics, Inorganic Chemical Industries Division, National Research Centre, Dokki, 12622 Cairo (Egypt); Liu, Xujie [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055 (China); Zhang, Ranran; Yang, Xing; Shen, Zhijian [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Feng, Qingling, E-mail: biomater@mail.tsinghua.edu.cn [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China)

    2016-10-01

    The aims of the present study were to develop boron-incorporated TiO{sub 2} coating (B-TiO{sub 2} coating) through micro-arc oxidation (MAO) and subsequently evaluate the effect of boron incorporation on the in vitro biological performance of the coatings. The physicochemical properties of B-TiO{sub 2} coating and its response to osteoblast like cells (SaOS-2) were investigated compared to the control group without boron (TiO{sub 2} coating). The morphological and X-ray diffraction results showed that both coatings exhibited similar surface topography and phase composition, respectively. However, the incorporation of B led to an enhancement in the surface hydrophilicity of B-TiO{sub 2} coating. The spreading of SaOS-2 cells on B-TiO{sub 2} coating was faster than that on TiO{sub 2} coating. The proliferation rate of SaOS-2 cells cultured on B-TiO{sub 2} decreased after 5 days of culture compared to that on TiO{sub 2} coating. SaOS-2 cells cultured on B-TiO{sub 2} coating exhibited an enhanced alkaline phosphatase (ALP) activity, Collagen I synthesis and in vitro mineralization compared to those on TiO{sub 2} coating. The present findings suggest that B-TiO{sub 2} coating is a promising candidate surface for orthopedic implants. - Highlights: • SaOS-2 cell response to pure TiO{sub 2} and B-TiO{sub 2} coatings was investigated. • Initial cell spreading on B-TiO{sub 2} coating was accelerated compared to that on TiO{sub 2} coating. • Cell proliferation on B-TiO{sub 2} coating was inhibited compared to that on TiO{sub 2} coating. • Cell differentiation on B-TiO{sub 2} coating was enhanced compared to that on TiO{sub 2} coating.

  9. Anti-citrullinated protein antibodies promote apoptosis of mature human Saos-2 osteoblasts via cell-surface binding to citrullinated heat shock protein 60.

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    Lu, Ming-Chi; Yu, Chia-Li; Yu, Hui-Chun; Huang, Hsien-Bin; Koo, Malcolm; Lai, Ning-Sheng

    2016-01-01

    We hypothesized that anti-citrullinated protein antibodies (ACPAs) react with osteoblast surface citrullinated proteins and affect cell function, leading to joint damage in patients with rheumatoid arthritis (RA). First, we purified ACPAs by cyclic citrullinated peptide (CCP)-conjugated affinity column chromatography. The cognate antigens of ACPAs on Saos-2 cells, a sarcoma osteogenic cell line generated from human osteoblasts, were probed by ACPAs, and the reactive bands were analyzed using proteomic analyses. We found that ACPAs bind to Saos-2 cell membrane, and several protein candidates, including HSP60, were identified. We then cloned and purified recombinant heat shock protein 60 (HSP60) and citrullinated HSP60 (citHSP60) and investigated the effect of ACPAs on Saos-2 cell. We confirmed that HSP60 obtained from Saos-2 cell membrane were citrullinated and reacted with ACPAs, which induces Saos-2 cells apoptosis via binding to surface-expressed citHSP60 through Toll-like receptor 4 signaling. ACPAs promoted interleukin (IL)-6 and IL-8 expression in Saos-2 cells. Finally, sera from patients with RA and healthy controls were examined for their titers of anti-HSP60 and anti-citHSP60 antibodies using an enzyme-linked immunosorbent assay. The radiographic change in patients with RA was evaluated using the Genant-modified Sharp scoring system. Patients with RA showed higher sera titers of anti-citHSP60, but not anti-HSP60, antibodies when compared with controls. In addition, the anti-citHSP60 level was positively associated with increased joint damage in patients with RA. In conclusion, Saos-2 cell apoptosis was mediated by ACPAs via binding to cell surface-expressed citHSP60 and the titer of anti-citHSP60 in patients with RA positively associated with joint damage. Copyright © 2015 Elsevier GmbH. All rights reserved.

  10. Thin films of single-walled carbon nanotubes promote human osteoblastic cells (Saos-2) proliferation in low serum concentrations

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    Akasaka, Tsukasa; Yokoyama, Atsuro; Matsuoka, Makoto; Hashimoto, Takeshi; Watari, Fumio

    2010-01-01

    One strategy used for the regeneration of bone is the development of cell culture substrates and scaffolds that can control osteoblast proliferation and differentiation. In recent investigations, carbon nanotubes (CNTs) have been utilized as scaffolds for osteoblastic cell cultures; however, there are only a few reports describing the proliferation of osteoblastic cells on thin CNT films; in particular, the effects of serum concentration on cell proliferation have not been studied. In the present study, we prepared culture dishes with homogeneous thin or thick films of non-modified CNTs and examined the effect of serum concentrations on human osteoblastic cells (Saos-2) proliferation in these culture dishes. We demonstrated that the ratio of cell proliferation was strongly affected by the concentration of serum. Interestingly, single-walled carbon nanotube (SWNT) thin films were found to be the most effective substrate for the proliferation of Saos-2 cells in low concentrations of serum. Thus, thin SWNT films may be used as an effective biomaterial for the culture of Saos-2 cells in low serum concentrations.

  11. Maturation of osteoblast-like SaoS2 induced by carbon nanotubes

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    Li Xiaoming; Uo, Motohiro; Akasaka, Tsukasa; Abe, Shigeaki; Watari, Fumio; Gao Hong; Sato, Yoshinori; Feng Qingling; Cui Fuzhai

    2009-01-01

    Osteogenic maturation of the osteoblast is crucial for bone formation. In this study, multi-walled carbon nanotubes (MWCNTs) and graphite (GP) were pressed as compacts. The greater ability of carbon nanotubes to adsorb proteins, compared with graphite, was shown. Human osteoblast-like SaoS2 cells were cultured and the cell response to the two kinds of compacts was compared in vitro. Meanwhile, we used cell culture on the culture plate as a control. Assays for osteonectin, osteopontin and osteocalcin gene expression, total protein (TP) amount, alkaline phosphatase activity (ALP) and DNA of cells cultured on the samples were done. During the conventional culture, significantly higher osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA on carbon nanotubes were found. To confirm the hypothesis that the larger amount of specific proteins adsorbed on the carbon nanotubes was crucial for this, the compacts were pre-soaked in culture medium having additional recombinant human bone morphogenetic protein-2 (rhBMP-2) before cell culture. Compared with GP, osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA of the cells tested increased more on the MWCNTs after the compacts were pre-soaked in the culture medium with rhBMP-2. The results indicated that the carbon nanotubes might induce osteogenic maturation of the osteoblast by adsorbing more specific proteins.

  12. Influence of SaOS-2 cells on corrosion behavior of cast Mg-2.0Zn0.98Mn magnesium alloy.

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    Witecka, Agnieszka; Yamamoto, Akiko; Święszkowski, Wojciech

    2017-02-01

    In this research, the effect of the presence of living cells (SaOS-2) on in vitro degradation of Mg-2.0Zn-0.98Mn (ZM21) magnesium alloy was examined by two methods simple immersion/cell culture tests and electrochemical measurements (electrochemical impedance spectroscopy and potentiodynamic polarization) under cell culture conditions. In immersion/cell culture tests, when SaOS-2 cells were cultured on ZM21 samples, pH of cell culture medium decreased, therefore weight loss and Mg 2+ ion release from the samples increased. Electrochemical measurements revealed the presence of living cells increased corrosion rate (I corr ) and decreased polarization resistance (R p ) after 48h of incubation. This acceleration of ZM21 corrosion can mainly be attributed to the decrease of medium pH due to cellular metabolic activities. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Characteristics of minerals in vesicles produced by human osteoblasts hFOB 1.19 and osteosarcoma Saos-2 cells stimulated for mineralization.

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    Strzelecka-Kiliszek, Agnieszka; Bozycki, Lukasz; Mebarek, Saida; Buchet, Rene; Pikula, Slawomir

    2017-06-01

    Bone cells control initial steps of mineralization by producing extracellular matrix (ECM) proteins and releasing vesicles that trigger apatite nucleation. Using transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) we compared the quality of minerals in vesicles produced by two distinct human cell lines: fetal osteoblastic hFOB 1.19 and osteosarcoma Saos-2. Both cell lines, subjected to osteogenic medium with ascorbic acid (AA) and β-glycerophosphate (β-GP), undergo the entire osteoblastic differentiation program from proliferation to mineralization, produce the ECM and spontaneously release vesicles. We observed that Saos-2 cells mineralized better than hFOB 1.19, as probed by Alizarin Red-S (AR-S) staining, tissue nonspecific alkaline phosphatase (TNAP) activity and by analyzing the composition of minerals in vesicles. Vesicles released from Saos-2 cells contained and were surrounded by more minerals than vesicles released from hFOB 1.19. In addition, there were more F and Cl substituted apatites in vesicles from hFOB 1.19 than in those from Saos-2 cells as determined by ion ratios. Saos-2 and h-FOB 1.19 cells revealed distinct mineralization profiles, indicating that the process of mineralization may proceed differently in various types of cells. Our findings suggest that TNAP activity is correlated with the relative proportions of mineral-filled vesicles and mineral-surrounded vesicles. The origin of vesicles and their properties predetermine the onset of mineralization at the cellular level. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Effect of human granulocyte macrophage-colony stimulating factor on differentiation and apoptosis of the human osteosarcoma cell line SaOS-2

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    L Postiglione

    2009-06-01

    Full Text Available We investigated the effects of human granulocyte macrophage- colony stimulating factor (GM-CSF on the relation between differentiation and apoptosis in SaOS-2 cells, an osteoblast-like cell line. To determine the relationship between these cellular processes, SaOS-2 cells were treated in vitro for 1, 7 and 14 days with 200 ng/mL GM-CSF and compared with untreated cells. Five nM insulin-like growth factor (IGF-I and 30 nM okadaic acid were used as negative and positive controls of apoptosis, respectively. Effects on cell differentiation were determined by ECM (extracellular matrix mineralization, morphology of some typical mature osteoblast differentiation markers, such as osteopontin and sialoprotein II (BSP-II, and production of bone ECM components such as collagen I. The results showed that treatment with GM-CSF caused cell differentiation accompanied by increased production of osteopontin and BSP-II, together with increased ECM deposition and mineralization. Flow cytometric analysis of annexin V and propidium iodide incorporation showed that GM-CSF up-regulated apoptotic cell death of SaOS-2 cells after 14 days of culture in contrast to okadaic acid, which stimulated SaOS-2 apoptosis only during the early period of culture. Endonucleolytic cleavage of genomic DNA, detected by “laddering analysis”, confirmed these data. The results suggest that GM-CSF induces osteoblastic differentiation and long-term apoptotic cell death of the SaOS-2 human osteosarcoma cell line, which in turn suggests a possible in vivo physiological role for GM-CSF on human osteoblast cells.

  15. Effect of Irradiation on Apoptosis, Cell Cycle Arrest and Calcified Nodule Formation of Rat Calvarial Osteoblast

    International Nuclear Information System (INIS)

    Lee, Young Mi; Choi, Hang Moon; Heo, Min Suk; Lee, Sam Sun; Choi, Soon Chul; Park, Tae Won

    2000-01-01

    The study was aimed to detect the induction of apoptosis, cell cycle arrest and calcified nodule formation after irradiation on primarily cultured osteoblasts. Using rat calvarial osteoblasts, the effects of irradiation on apoptosis, cell cycle arrest, and calcified nodule formation were studied. The single irradiation of 10, 20 Gy was done with 5.38 Gy/min dose rate using the 137 Cs cell irradiator at 4th and 14th day of culture. Apoptosis induction and cell cycle arrest were assayed by the flow cytometry at 1, 2, 3, and 4 days after irradiation. The formation of calcified nodules was observed by alizarin red staining at 1, 3, 10, 14 days after irradiation at 4th day of culture, and at 1, 4, 5 days after irradiation at 14th day of culture. Apoptosis was not induced by 10 or 20 Gy independent of irradiation and culture period. Irradiation did not induced G1 arrest in post-irradiated osteoblasts. After irradiation at 4th-day of culture, G2 arrest was induced but it was not statistically significant after irradiation at 14th-day of culture. In the case of irradiated cells at 4th day of culture, calcified nodules were not formed and at 14th-day of culture after irradiation, calcified nodule formation did not affected. Taken together, these results suggest that irradiation at the dose of 10-20 Gy would not affect apoptosis induction of osteoblasts. Cell cycle and calcified nodule formation were influenced by the level of differentiation of osteblasts.

  16. Sp1/Sp3 and DNA-methylation contribute to basal transcriptional activation of human podoplanin in MG63 versus Saos-2 osteoblastic cells

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    Puri Christina

    2007-03-01

    Full Text Available Abstract Background Podoplanin is a membrane mucin that, among a series of tissues, is expressed on late osteoblasts and osteocytes. Since recent findings have focussed on podoplanin's potential role as a tumour progression factor, we aimed at identifying regulatory elements conferring PDPN promoter activity. Here, we characterized the molecular mechanism controlling basal PDPN transcription in human osteoblast-like MG63 versus Saos-2 cells. Results We cloned and sequenced 2056 nucleotides from the 5'-flanking region of the PDPN gene and a computational search revealed that the TATA and CAAT box-lacking promoter possesses features of a growth-related gene, such as a GC-rich 5' region and the presence of multiple putative Sp1, AP-4 and NF-1 sites. Reporter gene assays demonstrated a functional promoter in MG63 cells exhibiting 30-fold more activity than in Saos-2 cells. In vitro DNase I footprinting revealed eight protected regions flanked by DNaseI hypersensitive sites within the region bp -728 to -39 present in MG63, but not in Saos-2 cells. Among these regions, mutation and supershift electrophoretic mobility shift assays (EMSA identified four Sp1/Sp3 binding sites and two binding sites for yet unknown transcription factors. Deletion studies demonstrated the functional importance of two Sp1/Sp3 sites for PDPN promoter activity. Overexpression of Sp1 and Sp3 independently increased the stimulatory effect of the promoter and podoplanin mRNA levels in MG63 and Saos-2 cells. In SL2 cells, Sp3 functioned as a repressor, while Sp1 and Sp3 acted positively synergistic. Weak PDPN promoter activity of Saos-2 cells correlated with low Sp1/Sp3 nuclear levels, which was confirmed by Sp1/Sp3 chromatin immunoprecipitations in vivo. Moreover, methylation-sensitive Southern blot analyses and bisulfite sequencing detected strong methylation of CpG sites upstream of bp -464 in MG63 cells, but hypomethylation of these sites in Saos-2 cells. Concomitantly

  17. Ghost cells in pilomatrixoma, craniopharyngioma, and calcifying cystic odontogenic tumor: histological, immunohistochemical, and ultrastructural study.

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    Rumayor, Alicia; Carlos, Román; Kirsch, Hernán Molina; de Andrade, Bruno A Benevenuto; Romañach, Mario J; de Almeida, Oslei Paes

    2015-04-01

    Pilomatrixoma, craniopharyngioma, and calcifying cystic odontogenic tumor are the main entities presenting ghost cells as an important histological feature, in spite their quite different clinical presentation; it seems that they share a common pathway in the formation of these cells. The aim of this study is to examine and compare the characteristics of ghost and other cells that form these lesions. Forty-three cases including 21 pilomatrixomas, 14 craniopharyngiomas, and eight calcifying cystic odontogenic tumors were evaluated by immunohistochemistry for cytokeratins, CD138, β-catenin, D2-40, Glut-1, FAS, CD10 and also by scanning electron microscopy. The CKs, CD138, β-catenin, Glut-1, FAS, and CD10 were more often expressed by transitional cells of craniopharyngioma and calcifying cystic odontogenic tumor, compared with pilomatrixoma. Basaloid cells of pilomatrixoma showed strong positivity for CD138 and CD10. Differences on expression pattern were identified in transitional and basal cells, as ghost cells were negative for most antibodies used, except by low expression for cytokeratins. By scanning electron microscopy, the morphology of ghost cells were similar in their fibrillar cytoplasm, but their pattern varied from sheets in pilomatrixoma to small clusters in craniopharyngioma and calcifying cystic odontogenic tumor. Mechanisms involved in formation of ghost cells are unknown, but probably they follow different pathways as protein expression in the basal/transitional cells was not uniform in the three tumors studied. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. Experimental study of inhibitory effects of diallyl trisulfide on the growth of human osteosarcoma Saos-2 cells by downregulating expression of glucose-regulated protein 78

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    Zhang Y

    2018-01-01

    Full Text Available Yue Zhang,1,* Wen-Peng Xie,1,* Yong-Kui Zhang,2 Yi-Qiang Chen,3 Dong-Li Wang,2 Gang Li,2 Dong-Hui Guan2 1First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China; 2Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China; 3Department of Orthopedics, The First People’s Hospital of Taian City, Taian, People’s Republic of China *These authors contributed equally to the paper Background: Diallyl trisulfide (DATS is a natural organic sulfur compound isolated from garlic that has good anticancer activity according to many previous reports. There are many studies pointing out that DATS can downregulate expression of the glucose-regulated protein 78 (GRP78, which is associated with poor prognosis and drug resistance in various types of human cancers. However, it remains unknown whether DATS has the same effect on human osteosarcoma cells. This study attempted to clarify the potential molecular mechanisms of the action of DATS in human osteosarcoma Saos-2 cells.Methods: We used an inverted phase microscope and immunofluorescent staining to observe the morphological changes of Saos-2 cells after being cultured in different concentrations of DATS (0, 25, 50, and 100 µM for 24 h, or for four time periods (24, 48, 72, and 96 h in the same DATS concentration (50 µM. Quantitative real-time polymerase chain reaction and Western blot were used to detect the expression level of GRP78 mRNA and proteins in Saos-2 cells. GRP78 expression was suppressed in Saos-2 cells by utilizing small-interfering RNA, and the cells were subsequently used to study the anti-proliferative effects of DATS treatment.Results: The expression level of GRP78 mRNA and proteins was significantly downregulated due to the increased concentration and effective times of DATS (P<0.05. In addition, there were significant associations between GRP78

  19. Interaction of human osteoblast-like Saos-2 cells with stainless steel coated by silicalite-1 films

    Czech Academy of Sciences Publication Activity Database

    Jirka, Ivan; Vandrovcová, Marta; Plšek, Jan; Bouša, Milan; Brabec, Libor; Dragounová, Helena; Bačáková, Lucie

    2017-01-01

    Roč. 76, JUL 2017 (2017), s. 775-781 ISSN 0928-4931 R&D Projects: GA ČR(CZ) GA16-02681S Institutional support: RVO:61388955 ; RVO:67985823 Keywords : silicalite-1 film * biocompatibility * human osteoblast-like Saos-2 cells Subject RIV: CF - Physical ; Theoretical Chemistry; EI - Biotechnology ; Bionics (FGU-C) OBOR OECD: Physical chemistry; Biomaterials (as related to medical implants, devices, sensors) (FGU-C)

  20. Engineering of hyaline cartilage with a calcified zone using bone marrow stromal cells.

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    Lee, W D; Hurtig, M B; Pilliar, R M; Stanford, W L; Kandel, R A

    2015-08-01

    In healthy joints, a zone of calcified cartilage (ZCC) provides the mechanical integration between articular cartilage and subchondral bone. Recapitulation of this architectural feature should serve to resist the constant shear force from the movement of the joint and prevent the delamination of tissue-engineered cartilage. Previous approaches to create the ZCC at the cartilage-substrate interface have relied on strategic use of exogenous scaffolds and adhesives, which are susceptible to failure by degradation and wear. In contrast, we report a successful scaffold-free engineering of ZCC to integrate tissue-engineered cartilage and a porous biodegradable bone substitute, using sheep bone marrow stromal cells (BMSCs) as the cell source for both cartilaginous zones. BMSCs were predifferentiated to chondrocytes, harvested and then grown on a porous calcium polyphosphate substrate in the presence of triiodothyronine (T3). T3 was withdrawn, and additional predifferentiated chondrocytes were placed on top of the construct and grown for 21 days. This protocol yielded two distinct zones: hyaline cartilage that accumulated proteoglycans and collagen type II, and calcified cartilage adjacent to the substrate that additionally accumulated mineral and collagen type X. Constructs with the calcified interface had comparable compressive strength to native sheep osteochondral tissue and higher interfacial shear strength compared to control without a calcified zone. This protocol improves on the existing scaffold-free approaches to cartilage tissue engineering by incorporating a calcified zone. Since this protocol employs no xenogeneic material, it will be appropriate for use in preclinical large-animal studies. Copyright © 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

  1. Hydroxyapatite and Calcified Elastin Induce Osteoblast-like Differentiation in Rat Aortic Smooth Muscle Cells

    Science.gov (United States)

    Lei, Yang; Sinha, Aditi; Nosoudi, Nasim; Grover, Ankit; Vyavahare, Naren

    2014-01-01

    Vascular calcification can be categorized into two different types. Intimal calcification related to atherosclerosis and elastin-specific medial arterial calcification (MAC). Osteoblast-like differentiation of vascular smooth muscle cells (VSMCs) has been shown in both types; however, how this relates to initiation of vascular calcification is unclear. We hypothesize that the initial deposition of hydroxyapatite-like mineral in MAC occurs on degraded elastin first and that causes osteogenic transformation of VSMCs. To test this, rat aortic smooth muscle cells (RASMCs) were cultured on hydroxyapatite crystals and calcified aortic elastin. Using RT-PCR and specific protein assays, we demonstrate that RASMCs lose their smooth muscle lineage markers like alpha smooth muscle actin (SMA) and myosin heavy chain (MHC) and undergo chondrogenic/osteogenic transformation. This is indicated by an increase in the expression of typical chondrogenic proteins such as aggrecan, collagen type II alpha 1(Col2a1) and bone proteins such as runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP) and osteocalcin (OCN). Furthermore, when calcified conditions are removed, cells return to their original phenotype. Our data supports the hypothesis that elastin degradation and calcification precedes VSMCs' osteoblast-like differentiation. PMID:24447384

  2. Response of SAOS-2 cells to simulated microgravity and effect of biocompatible sol-gel hybrid coatings

    Science.gov (United States)

    Catauro, M.; Bollino, F.; Papale, F.

    2016-05-01

    The health of astronauts, during space flight, is threatened by bone loss induced by microgravity, mainly attributed to an imbalance in the bone remodeling process. In the present work, the response to the microgravity of bone cells has been studied using the SAOS-2 cell line grown under the condition of weightlessness, simulated by means of a Random Positioning Machine (RPM). Cell viability after 72 h of rotation has been evaluated by means of WST-8 assay and compared to that of control cells. Although no significant difference between the two cell groups has been observed in terms of viability, F-actin staining showed that microgravity environment induces cell apoptosis and altered F-actin organization. To investigate the possibility of hindering the trend of the cells towards the death, after 72 h of rotation the cells have been seeded onto biocompatible ZrO2/PCL hybrid coatings, previously obtained using a sol-gel dip coating procedure. WST-8 assay, carried out after 24 h, showed that the materials are able to inhibit the pro-apoptotic effect of microgravity on cells.

  3. The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

    Czech Academy of Sciences Publication Activity Database

    Filová, Elena; Fojt, J.; Krýslová, Markéta; Moravec, H.; Joska, L.; Bačáková, Lucie

    2015-01-01

    Roč. 10, 20 Nov (2015), s. 7145-7163 E-ISSN 1178-2013 R&D Projects: GA MZd(CZ) NT13297; GA ČR(CZ) GA15-01558S; GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:67985823 Keywords : nanostructure * titanium nanotubes * cell adhesion * Saos-2 cells * osteogenic differentiation Subject RIV: EI - Biotechnology ; Bionics Impact factor: 4.320, year: 2015

  4. Engineering a morphogenetically active hydrogel for bioprinting of bioartificial tissue derived from human osteoblast-like SaOS-2 cells.

    Science.gov (United States)

    Neufurth, Meik; Wang, Xiaohong; Schröder, Heinz C; Feng, Qingling; Diehl-Seifert, Bärbel; Ziebart, Thomas; Steffen, Renate; Wang, Shunfeng; Müller, Werner E G

    2014-10-01

    Sodium alginate hydrogel, stabilized with gelatin, is a suitable, biologically inert matrix that can be used for encapsulating and 3D bioprinting of bone-related SaOS-2 cells. However, the cells, embedded in this matrix, remain in a non-proliferating state. Here we show that addition of an overlay onto the bioprinted alginate/gelatine/SaOS-2 cell scaffold, consisting of agarose and the calcium salt of polyphosphate [polyP·Ca(2+)-complex], resulted in a marked increase in cell proliferation. In the presence of 100 μm polyP·Ca(2+)-complex, the cells proliferate with a generation time of approximately 47-55 h. In addition, the hardness of the alginate/gelatin hydrogel substantially increases in the presence of the polymer. The reduced Young's modulus for the alginate/gelatin hydrogel is approximately 13-14 kPa, and this value drops to approximately 0.5 kPa after incubation of the cell containing scaffolds for 5 d. In the presence of 100 μm polyP·Ca(2+)-complex, the reduced Young's modulus increases to about 22 kPa. The hardness of the polyP·Ca(2+)-complex containing hydrogel remains essentially constant if cells are absent in the matrix, but it drops to 3.2 kPa after a 5 d incubation period in the presence of SaOS-2 cells, indicating that polyP·Ca(2+)-complex becomes metabolized, degraded, by the cells. The alginate/gelatine-agarose system with polyP·Ca(2+)-complex cause a significant increase in the mineralization of the cells. SEM analyses revealed that the morphology of the mineral nodules formed on the surface of the cells embedded in the alginate/gelatin hydrogel do not significantly differ from the nodules on cells growing in monolayer cultures. The newly developed technique, using cells encapsulated into an alginate/gelatin hydrogel and a secondary layer containing the morphogenetically active, growth promoting polymer polyP·Ca(2+)-complex opens new possibilities for the application of 3D bioprinting in bone tissue engineering. Copyright

  5. Aortic calcified particles modulate valvular endothelial and interstitial cells

    NARCIS (Netherlands)

    van Engeland, N.C.A.; Bertazzo, S.; Sarathchandra, P.; McCormack, A.; Bouten, C.V.C.; Yacoub, M.H.; Chester, A.H.; Latif, N.

    2017-01-01

    BACKGROUND: Normal and calcified human valve cusps, coronary arteries, and aortae harbor spherical calcium phosphate microparticles of identical composition and crystallinity, and their role remains unknown. OBJECTIVE: The objective was to examine the direct effects of isolated calcified particles

  6. Comparison of the Influence of Phospholipid-Coated Porous Ti-6Al-4V Material on the Osteosarcoma Cell Line Saos-2 and Primary Human Bone Derived Cells

    Directory of Open Access Journals (Sweden)

    Axel Deing

    2016-03-01

    Full Text Available Biomaterial surface functionalization remains of great interest in the promotion of cell osteogenic induction. Previous studies highlighted the positive effects of porous Ti-6Al-4V and phospholipid coating on osteoblast differentiation and bone remodeling. Therefore, the first objective of this study was to evaluate the potential synergistic effects of material porosity and phospholipid coating. Primary human osteoblasts and Saos-2 cells were cultured on different Ti-6Al-4V specimens (mirror-like polished or porous specimens and were coated or not with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE for three weeks or five weeks. Selected gene expressions (e.g., classical bone markers: alkaline phosphatase, osteocalcin, osteoprotegerin (OPG, receptor activator of nuclear factor kappa-β ligand (RANKL and runt-related transcription factor 2 were estimated in vitro. Furthermore, the expressions of osteocalcin and osteopontin were examined via fluorescent microscopy at five weeks (immunocytochemistry. Consequently, it was observed that phospholipid coating potentiates preferences for low and high porosities in Saos-2 and primary cells, respectively, at the gene and protein levels. Additionally, RANKL and OPG exhibited different gene expression patterns; primary cells showed dramatically increased RANKL expression, whereas OPG expression was decreased in the presence of POPE. A synergistic effect of increased porosity and phospholipid coating was observed in primary osteoblasts in bone remodeling. This study showed the advantage of primary cells over the standard bone cell model.

  7. Two cases report of Calcifying Odontogenic Cyst

    International Nuclear Information System (INIS)

    Lee, Byung Do; Lee, Wan; Paeng, Jun Young; Lee, Jun; Choi, Moon Ki; Son, Hyun Jin

    2009-01-01

    The calcifying odontogenic cyst (COC) is a rare disorder of the jaws and shows various radiographic features. The purpose of this study is to describe the different radiographic appearances of 2 cases of COC. Case 1 was located in the posterior maxilla extending into maxillary sinus, showing unilocular radiolucency with a well-defined margin. Cortical bone expansion and thinning were prominent. Root resorption of adjacent teeth was apparent. Case 2 showed unilocular radiolucency with a calcified material. Calcification was supposed to be dystrophic dental hard structures, detected at the periphery of the lesion. Ghost cell and proliferation of ameloblastoma-like tissues were common features for these two lesions on histopathological findings. This reports presented common and atypical radiographic features of the COC.

  8. Ameloblastomatous calcifying odontogenic cyst: A rare histologic variant

    Directory of Open Access Journals (Sweden)

    Basavaraj N Kallalli

    2015-01-01

    Full Text Available Ameloblastoma is a well-known odontogenic tumor that can be associated with calcifying odontogenic cysts (COCs, but only a few reports give its clinical and radiographic features. Calcifying odontogenic cyst was first categorized as a distinct entity by Gorlin et al., and has been named after him since then. Calcifying odontogenic cyst is an uncommon developmental odontogenic lesion that demonstrates histopathologic diversity. It is well known that this lesion can occur in association with odontogenic tumors such as complex odontoma and ameloblastoma. The term COC was not included by the World Health Organization (WHO in its report of 2005 and is called calcifying cystic odontogenic tumor (CCOT. Histopathologic examination of ameloblastomatous CCOT reveals ameloblastic islands containing ghost cells. Although association of ameloblastoma with this lesion is important, only a few cases have been reported in literature. The present case report is of ameloblastomatous calcifying cystic odontogenic tumor, a rare histologic variant, in a 20-year-old male patient in the left mandibular posterior region.

  9. How ocean acidification can benefit calcifiers.

    Science.gov (United States)

    Connell, Sean D; Doubleday, Zoë A; Hamlyn, Sarah B; Foster, Nicole R; Harley, Christopher D G; Helmuth, Brian; Kelaher, Brendan P; Nagelkerken, Ivan; Sarà, Gianluca; Russell, Bayden D

    2017-02-06

    Reduction in seawater pH due to rising levels of anthropogenic carbon dioxide (CO 2 ) in the world's oceans is a major force set to shape the future of marine ecosystems and the ecological services they provide [1,2]. In particular, ocean acidification is predicted to have a detrimental effect on the physiology of calcifying organisms [3]. Yet, the indirect effects of ocean acidification on calcifying organisms, which may counter or exacerbate direct effects, is uncertain. Using volcanic CO 2 vents, we tested the indirect effects of ocean acidification on a calcifying herbivore (gastropod) within the natural complexity of an ecological system. Contrary to predictions, the abundance of this calcifier was greater at vent sites (with near-future CO 2 levels). Furthermore, translocation experiments demonstrated that ocean acidification did not drive increases in gastropod abundance directly, but indirectly as a function of increased habitat and food (algal biomass). We conclude that the effect of ocean acidification on algae (primary producers) can have a strong, indirect positive influence on the abundance of some calcifying herbivores, which can overwhelm any direct negative effects. This finding points to the need to understand ecological processes that buffer the negative effects of environmental change. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Differential responses of calcifying and non-calcifying epibionts of a brown macroalga to present-day and future upwelling pCO2.

    Directory of Open Access Journals (Sweden)

    Vincent Saderne

    Full Text Available Seaweeds are key species of the Baltic Sea benthic ecosystems. They are the substratum of numerous fouling epibionts like bryozoans and tubeworms. Several of these epibionts bear calcified structures and could be impacted by the high pCO2 events of the late summer upwellings in the Baltic nearshores. Those events are expected to increase in strength and duration with global change and ocean acidification. If calcifying epibionts are impacted by transient acidification as driven by upwelling events, their increasing prevalence could cause a shift of the fouling communities toward fleshy species. The aim of the present study was to test the sensitivity of selected seaweed macrofoulers to transient elevation of pCO2 in their natural microenvironment, i.e. the boundary layer covering the thallus surface of brown seaweeds. Fragments of the macroalga Fucus serratus bearing an epibiotic community composed of the calcifiers Spirorbis spirorbis (Annelida and Electra pilosa (Bryozoa and the non-calcifier Alcyonidium hirsutum (Bryozoa were maintained for 30 days under three pCO2 conditions: natural 460 ± 59 µatm, present-day upwelling1193 ± 166 µatm and future upwelling 3150 ± 446 µatm. Only the highest pCO2 caused a significant reduction of growth rates and settlement of S. spirorbis individuals. Additionally, S. spirorbis settled juveniles exhibited enhanced calcification of 40% during daylight hours compared to dark hours, possibly reflecting a day-night alternation of an acidification-modulating effect by algal photosynthesis as opposed to an acidification-enhancing effect of algal respiration. E. pilosa colonies showed significantly increased growth rates at intermediate pCO2 (1193 µatm but no response to higher pCO2. No effect of acidification on A. hirsutum colonies growth rates was observed. The results suggest a remarkable resistance of the algal macro-epibionts to levels of acidification occurring at present day upwellings in the Baltic

  11. A Sclerostin super-producer cell line derived from the human cell line SaOS-2: a new tool for the study of the molecular mechanisms driving Sclerostin expression.

    Science.gov (United States)

    Pérez-Campo, Flor M; Sañudo, Carolina; Delgado-Calle, Jesús; Arozamena, Jana; Zarrabeitia, María T; Riancho, José A

    2014-08-01

    Sclerostin, the product of the SOST gene, is a key regulator of bone homeostasis. Sclerostin interferes with the Wnt signalling pathway and, therefore, has a negative effect on bone formation. Although the importance of sclerostin in bone homeostasis is well established, many aspects of its biology are still unknown. Due to its restricted pattern of expression, in vitro studies of SOST gene regulation are technically challenging. Furthermore, a more profound investigation of the molecular mechanism controlling sclerostin expression has been hampered by the lack of a good human in vitro model. Here, we describe two cell lines derived from the human osteosarcoma cell line SaOS-2 that produce elevated levels of sclerostin. Analysis of the super-producer cell lines showed that sclerostin levels were still reduced in response to parathyroid hormone treatment or in response to mechanical loading, indicating that these regulatory mechanisms were not affected in the presented cell lines. In addition, we did not find differences between the promoter or ECR5 sequences of our clones and the SaOS-2 parental line. However, the methylation of the proximal CpG island located at the SOST promoter was lower in the super-producer clones, in agreement with a higher level of SOST transcription. Although the underlying biological causes of the elevated levels of sclerostin production in this cell line are not yet clear, we believe that it could be an extremely useful tool to study the molecular mechanisms driving sclerostin expression in humans.

  12. Detection of hydroxyapatite in calcified cardiovascular tissues.

    Science.gov (United States)

    Lee, Jae Sam; Morrisett, Joel D; Tung, Ching-Hsuan

    2012-10-01

    The objective of this study is to develop a method for selective detection of the calcific (hydroxyapatite) component in human aortic smooth muscle cells in vitro and in calcified cardiovascular tissues ex vivo. This method uses a novel optical molecular imaging contrast dye, Cy-HABP-19, to target calcified cells and tissues. A peptide that mimics the binding affinity of osteocalcin was used to label hydroxyapatite in vitro and ex vivo. Morphological changes in vascular smooth muscle cells were evaluated at an early stage of the mineralization process induced by extrinsic stimuli, osteogenic factors and a magnetic suspension cell culture. Hydroxyapatite components were detected in monolayers of these cells in the presence of osteogenic factors and a magnetic suspension environment. Atherosclerotic plaque contains multiple components including lipidic, fibrotic, thrombotic, and calcific materials. Using optical imaging and the Cy-HABP-19 molecular imaging probe, we demonstrated that hydroxyapatite components could be selectively distinguished from various calcium salts in human aortic smooth muscle cells in vitro and in calcified cardiovascular tissues, carotid endarterectomy samples and aortic valves, ex vivo. Hydroxyapatite deposits in cardiovascular tissues were selectively detected in the early stage of the calcification process using our Cy-HABP-19 probe. This new probe makes it possible to study the earliest events associated with vascular hydroxyapatite deposition at the cellular and molecular levels. This target-selective molecular imaging probe approach holds high potential for revealing early pathophysiological changes, leading to progression, regression, or stabilization of cardiovascular diseases. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  13. LibKiSAO: a Java library for Querying KiSAO.

    Science.gov (United States)

    Zhukova, Anna; Adams, Richard; Laibe, Camille; Le Novère, Nicolas

    2012-09-24

    The Kinetic Simulation Algorithm Ontology (KiSAO) supplies information about existing algorithms available for the simulation of Systems Biology models, their characteristics, parameters and inter-relationships. KiSAO enables the unambiguous identification of algorithms from simulation descriptions. Information about analogous methods having similar characteristics and about algorithm parameters incorporated into KiSAO is desirable for simulation tools. To retrieve this information programmatically an application programming interface (API) for KiSAO is needed. We developed libKiSAO, a Java library to enable querying of the KiSA Ontology. It implements methods to retrieve information about simulation algorithms stored in KiSAO, their characteristics and parameters, and methods to query the algorithm hierarchy and search for similar algorithms providing comparable results for the same simulation set-up. Using libKiSAO, simulation tools can make logical inferences based on this knowledge and choose the most appropriate algorithm to perform a simulation. LibKiSAO also enables simulation tools to handle a wider range of simulation descriptions by determining which of the available methods are similar and can be used instead of the one indicated in the simulation description if that one is not implemented. LibKiSAO enables Java applications to easily access information about simulation algorithms, their characteristics and parameters stored in the OWL-encoded Kinetic Simulation Algorithm Ontology. LibKiSAO can be used by simulation description editors and simulation tools to improve reproducibility of computational simulation tasks and facilitate model re-use.

  14. Calcifying Fibrous Pseudotumor of the Esophagus

    Directory of Open Access Journals (Sweden)

    Shou-Wu Lee

    2010-11-01

    Full Text Available Calcifying fibrous pseudotumor is an uncommon lesion and has recently been recognized as a distinctive fibrous lesion. Esophageal calcifying fibrous pseudotumor is extremely rare and, to the best of our knowledge, has never been reported before. A 54-year-old woman underwent upper gastrointestinal endoscopy and endoscopic ultrasound because of intermittent dysphagia. The results showed 1 isoechoic esophageal submucosal tumor over the deep mucosa and submucosal layers, with calcifications inside. The patient underwent tumor excision, and the diagnosis was confirmed by pathological features, with abundant collagen, calcification and inflammatory cell infiltration. She received regular follow-up at the clinic and no evidence of tumor recurrence was found.

  15. Calcifying nanoparticles associated encrusted urinary bladder cystitis

    Directory of Open Access Journals (Sweden)

    Tomislav M Jelic

    2008-10-01

    Full Text Available Tomislav M Jelic1, Rod Roque1, Uzay Yasar2, Shayna B Tomchin1, Jose M Serrato2, Samuel G Deem3, James P Tierney3, Ho-Huang Chang11Department of Pathology Charleston Area Medical Center, Charleston WV, USA; 2Urology Center of Charleston, Charleston WV, USA; 3Urologic-Surgical Assoc. of Charleston, Charleston WV, USAAbstract: Encrusted cystitis is a subtype of chronic cystitis characterized by multiple calcifications in the form of plaques located in the interstitium of the urinary bladder mucosa and frequently associated with mucosal ulcers. It is a very rare disease of controversial etiology. Our transmission electron microscopy of the calcified plaques of encrusted cystitis has revealed that the smallest formed particles (elementary units of these calcifications are electron-dense shells surrounding an electron lucent core, diagnostic of calcifying nanoparticles (previously called nanobacteria. We pioneer the notion that calcifying nanoparticles are the causative agents of encrusted urinary bladder cystitis.Keywords: calcifying nanoparticles, nanobacteria, encrusted cystitis

  16. Notch signaling and ghost cell fate in the calcifying cystig odontogenic tumor

    Directory of Open Access Journals (Sweden)

    Siar CH

    2011-11-01

    Full Text Available Abstract Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites. Although GCs are present in a variety of odontogenic lesions notably the calcifying cystic odontogenic tumor (GCOT, their nature and process of formation remains elusive. The aim of this study was to investigate the role of Notch signaling in the cell fate specification of GCs in CCOT. Immunohistochemical staining for four Notch receptors (Notch1, Notch2, Notch3 and Notch4 and three ligands (Jagged1, Jagged2 and Delta1 was performed on archival tissues of five CCOT cases. Level of positivity was quantified as negative (0, mild (+, moderate (2+ and strong (3+. Results revealed that GCs demonstrated overexpression for Notch1 and Jagged1 suggesting that Notch1Jagged1 signaling might serve as the main transduction mechanism in cell fate decision for GCs in CCOT. Protein localizations were largely membranous and/or cytoplasmic. Mineralized GCs also stained positive implicating that the calcification process might be associated with upregulation of these molecules. The other Notch receptors and ligands were weak to absent in GCs and tumoral epithelium. Stromal endothelium and fibroblasts were stained variably positive.

  17. Non-calcifying and Langerhans cell-rich variant of calcifying epithelial odontogenic tumor

    Directory of Open Access Journals (Sweden)

    Hung-Pin Lin

    2016-06-01

    Full Text Available This study reported the clinicopathological features, treatment and prognosis of nine cases of noncalcifying and Langerhans cell (LC-rich calcifying epithelial odontogenic tumor (CEOT collected from the English literature. Of the nine cases, seven were intraosseous and two were extraosseous. All nine tumors were found in Asian patients. The age of the nine patients ranged from 20 years to 58 years with a mean age of 41 years. There were five female and four male patients. The seven intraosseous cases included six in the anterior and premolar region of the maxilla and one in the posterior region and ascending ramus of the mandible. The two extraosseous cases were located at the upper lateral incisor and premolar gingivae, respectively. Of the seven intraosseous cases, five showed unilocular and two multilocular radiolucency without foci of calcification. Six of the seven intraosseous cases showed resorption of the tooth roots in the tumor-involved region. Histologically, noncalcifying and LC-rich CEOTs were composed of small nests and thin strands of tumor epithelial cells with a relatively high number of LCs among them. This was the reason why we classed these nine cases as noncalcifying and LC-rich CEOTs. Two extraosseous cases received total excision of the gingival mass. For the seven intraosseous cases, four accepted partial maxillectomy or mandibulectomy, two received total excision or enucleation, and one underwent curettage. The six cases with the follow-up information available showed no tumor recurrence after a follow-up period of 6 months to 10 years.

  18. Over-calcified forms of the coccolithophore Emiliania huxleyi in high-CO2 waters are not preadapted to ocean acidification

    Science.gov (United States)

    von Dassow, Peter; Díaz-Rosas, Francisco; Mahdi Bendif, El; Gaitán-Espitia, Juan-Diego; Mella-Flores, Daniella; Rokitta, Sebastian; John, Uwe; Torres, Rodrigo

    2018-03-01

    Marine multicellular organisms inhabiting waters with natural high fluctuations in pH appear more tolerant to acidification than conspecifics occurring in nearby stable waters, suggesting that environments of fluctuating pH hold genetic reservoirs for adaptation of key groups to ocean acidification (OA). The abundant and cosmopolitan calcifying phytoplankton Emiliania huxleyi exhibits a range of morphotypes with varying degrees of coccolith mineralization. We show that E. huxleyi populations in the naturally acidified upwelling waters of the eastern South Pacific, where pH drops below 7.8 as is predicted for the global surface ocean by the year 2100, are dominated by exceptionally over-calcified morphotypes whose distal coccolith shield can be almost solid calcite. Shifts in morphotype composition of E. huxleyi populations correlate with changes in carbonate system parameters. We tested if these correlations indicate that the hyper-calcified morphotype is adapted to OA. In experimental exposures to present-day vs. future pCO2 (400 vs. 1200 µatm), the over-calcified morphotypes showed the same growth inhibition (-29.1±6.3 %) as moderately calcified morphotypes isolated from non-acidified water (-30.7±8.8 %). Under the high-CO2-low-pH condition, production rates of particulate organic carbon (POC) increased, while production rates of particulate inorganic carbon (PIC) were maintained or decreased slightly (but not significantly), leading to lowered PIC / POC ratios in all strains. There were no consistent correlations of response intensity with strain origin. The high-CO2-low-pH condition affected coccolith morphology equally or more strongly in over-calcified strains compared to moderately calcified strains. High-CO2-low-pH conditions appear not to directly select for exceptionally over-calcified morphotypes over other morphotypes, but perhaps indirectly by ecologically correlated factors. More generally, these results suggest that oceanic planktonic

  19. Food supply confers calcifiers resistance to ocean acidification

    KAUST Repository

    Ramajo, Laura; Pé rez-Leó n, Elia; Hendriks, Iris E.; Marbà , Nú ria; Krause-Jensen, Dorte; Sejr, Mikael K.; Blicher, Martin E.; Lagos, Nelson A.; Olsen, Ylva S.; Duarte, Carlos M.

    2016-01-01

    Invasion of ocean surface waters by anthropogenic CO2 emitted to the atmosphere is expected to reduce surface seawater pH to 7.8 by the end of this century compromising marine calcifiers. A broad range of biological and mineralogical mechanisms allow marine calcifiers to cope with ocean acidification, however these mechanisms are energetically demanding which affect other biological processes (trade-offs) with important implications for the resilience of the organisms against stressful conditions. Hence, food availability may play a critical role in determining the resistance of calcifiers to OA. Here we show, based on a meta-analysis of existing experimental results assessing the role of food supply in the response of organisms to OA, that food supply consistently confers calcifiers resistance to ocean acidification.

  20. Food supply confers calcifiers resistance to ocean acidification

    KAUST Repository

    Ramajo, Laura

    2016-01-18

    Invasion of ocean surface waters by anthropogenic CO2 emitted to the atmosphere is expected to reduce surface seawater pH to 7.8 by the end of this century compromising marine calcifiers. A broad range of biological and mineralogical mechanisms allow marine calcifiers to cope with ocean acidification, however these mechanisms are energetically demanding which affect other biological processes (trade-offs) with important implications for the resilience of the organisms against stressful conditions. Hence, food availability may play a critical role in determining the resistance of calcifiers to OA. Here we show, based on a meta-analysis of existing experimental results assessing the role of food supply in the response of organisms to OA, that food supply consistently confers calcifiers resistance to ocean acidification.

  1. A Hard Ball for a Tennis Player: A Rare Case of Large Calcifying Sertoli Cell Testicular Tumor

    Directory of Open Access Journals (Sweden)

    Simone Albisinni

    2017-07-01

    Full Text Available A 46 year old tennis player was addressed to our clinic after incidental finding of right testicular calcification on plain x-ray of the spine. Urologic consultation revealed a hard non-tender testicular mass which required inguinal orchiectomy. Final histology revealed large cell calcifying Sertoli cell tumor: we herein present the case and review current physiopathology of such rare testicular disease.

  2. Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings.

    Science.gov (United States)

    Filova, Elena; Vandrovcova, Marta; Jelinek, Miroslav; Zemek, Josef; Houdkova, Jana; Jan Remsa; Kocourek, Tomas; Stankova, Lubica; Bacakova, Lucie

    2017-01-01

    Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0-1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion; however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation.

  3. Light Curve Analysis of SAO23229

    Directory of Open Access Journals (Sweden)

    Ho-Il Kim

    1993-06-01

    Full Text Available We have made UBV light curves of a newly discovered eclipsing binary, SAO23229 at Sobaeksan Astronomy Observatory. We determined a minimum light time of HJD2448636.1170+/-0.0005 that is 3 minutes later than predicted time, and founda peculiar light variation at phase 0.75 that may not be secondary eclipse. Orbital period of SAO23229 would be 4.2 days rather than 2.1 days. Our analysis of the light curves shows that SAO23229 has a detached configuration consisting of two almost identical F type main sequence stars.

  4. Hyperthermia effects in the presence of gold nanoparticles together with chemotherapy on Saos-2 cell line

    International Nuclear Information System (INIS)

    Sazgarnia, A.; Bahreyni Toosi, M. H.; Haji Ghahremani, F.; Rajabi, O.; Aledavood, A.; Esmaily, H.

    2011-01-01

    Hyperthermia created by microwave, infrared, ultrasound and other methods, is often utilized as an adjuvant to sensitize cancer cells to the effects of chemotherapy and radiation therapy. We investigated the efficacy of hyperthermia using microwave in synergy with chemotherapy in the presence and absence and gold nanoparticles. Material and Methods: After culturing and proliferation of the Saos-2 cell line derived from human osteogenic sarcoma, the cells were incubated at two concentrations of gold nanoparticles in two diameters of 20 and 40 nm and in the absence and presence of doxorubicin in different groups. Forty eight hours after irradiating the cells with microwave up to a temperature of 42 d egree C , cell survival rate was determined using the MTT method, in order to study the effectiveness of the therapeutic parameters. Results: Cell survival in the presence of gold nanoparticles was greater than 95%. After chemotherapy by doxorubicin with and without 40 nm gold nanoparticles, cell survival rates were determined as 62.8% and 37.1 %, declining down to 17% and 4.1% respectively following the combined treatment with microwave and chemotherapy in the presence of 20 and 40 nm gold nanoparticles. Discussion and Conclusions: Gold nanoparticles did not induce any cytotoxicity by themselves; their presence along with microwave provided a reduction in survival rate that was comparable in severity with the lethal effects of doxorubicin. microwave hyperthermia with gold nanoparticles produced a higher treatment efficiency in comparison to similar groups in which gold nanoparticles were absent. The synergism observed between hyperthermia and chemotherapy was dependent in gold nanoparticles' size and concentration. This finding could be caused by increased uptake of doxorubicin by the cells in the presence of gold nanoparticles.

  5. Orbital atherectomy as an adjunct to debulk difficult calcified lesions prior to mesenteric artery stenting.

    Science.gov (United States)

    Manunga, Jesse M; Oderich, Gustavo S

    2012-08-01

    To describe a technique in which percutaneous orbital atherectomy is used to debulk heavily calcified superior mesenteric artery (SMA) occlusions as an adjunct in patients undergoing angioplasty and stenting. The technique is demonstrated in a 62-year-old woman with a replaced right hepatic artery originating from an SMA occluded by densely calcified lesions. Via a left transbrachial approach, a 7-F MPA guide catheter was used to engage the ostium of the SMA, which was crossed using a catheter and guidewire. The calcified lesion was debulked using the 2-mm Diamondback 360° orbital atherectomy system. The wire was exchanged for a 0.014-inch filter wire and 0.018-inch guidewire. Using a 2-guidewire technique, the SMA was stented with a self-expanding stent for the distal lesion that crossed side branches and a balloon-expandable stent at the ostium. A 0.014-inch guidewire was placed into the replaced hepatic artery through a cell of the self-expanding stent, followed by deployment of a small balloon-expandable stent to address the residual lesion. The use of orbital atherectomy to debulk occluded and heavily calcified SMA lesions may optimize the technical results with angioplasty and stenting.

  6. Calcified adrenal cyst

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Chung Kyu; Choi, Byung Sook [Yonsei University College of Medicine, Seoul (Korea, Republic of)

    1970-10-15

    Calcified hemorrhagic adrenal cysts are rather rare and unusual pathologic entity. Especially, the peripheral curvilinear calcification on roentgenogram is fairly characteristic picture of the cysts. Recently, we have experienced in Severance Hospital one of the classical cases of the benign calcified adrenal cyst in 35 year old white mail patient who has had vague abdominal pain and palpable mass in right abdomen. It has been reviewed several reports for adrenal cysts and hoped that this report may call additional attention of radiological diagnosis on this unusual disease.

  7. Iterative model reconstruction reduces calcified plaque volume in coronary CT angiography

    Energy Technology Data Exchange (ETDEWEB)

    Károlyi, Mihály, E-mail: mihaly.karolyi@cirg.hu [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Szilveszter, Bálint, E-mail: szilveszter.balint@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Kolossváry, Márton, E-mail: martonandko@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Takx, Richard A.P, E-mail: richard.takx@gmail.com [Department of Radiology, University Medical Center Utrecht, 100 Heidelberglaan, 3584, CX Utrecht (Netherlands); Celeng, Csilla, E-mail: celengcsilla@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Bartykowszki, Andrea, E-mail: bartyandi@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Jermendy, Ádám L., E-mail: adam.jermendy@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Panajotu, Alexisz, E-mail: panajotualexisz@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); Karády, Júlia, E-mail: karadyjulia@gmail.com [MTA-SE Cardiovascular Imaging Research Group, Heart and Vascular Center, Semmelweis University, 68. Varosmajor st, 1122, Budapest (Hungary); and others

    2017-02-15

    Objective: To assess the impact of iterative model reconstruction (IMR) on calcified plaque quantification as compared to filtered back projection reconstruction (FBP) and hybrid iterative reconstruction (HIR) in coronary computed tomography angiography (CTA). Methods: Raw image data of 52 patients who underwent 256-slice CTA were reconstructed with IMR, HIR and FBP. We evaluated qualitative, quantitative image quality parameters and quantified calcified and partially calcified plaque volumes using automated software. Results: Overall qualitative image quality significantly improved with HIR as compared to FBP, and further improved with IMR (p < 0.01 all). Contrast-to-noise ratios were improved with IMR, compared to HIR and FBP (51.0 [43.5–59.9], 20.3 [16.2–25.9] and 14.0 [11.2–17.7], respectively, all p < 0.01) Overall plaque volumes were lowest with IMR and highest with FBP (121.7 [79.3–168.4], 138.7 [90.6–191.7], 147.0 [100.7–183.6]). Similarly, calcified volumes (>130 HU) were decreased with IMR as compared to HIR and FBP (105.9 [62.1–144.6], 110.2 [63.8–166.6], 115.9 [81.7–164.2], respectively, p < 0.05 all). High-attenuation non-calcified volumes (90–129 HU) yielded similar values with FBP and HIR (p = 0.81), however it was lower with IMR (p < 0.05 both). Intermediate- (30–89 HU) and low-attenuation (<30 HU) non-calcified volumes showed no significant difference (p = 0.22 and p = 0.67, respectively). Conclusions: IMR improves image quality of coronary CTA and decreases calcified plaque volumes.

  8. A Case of Old Calcifying Epithelioma Processed Symptomless over 40 Years

    Directory of Open Access Journals (Sweden)

    Shinji Yamaguchi

    2013-01-01

    Full Text Available Calcifying epithelioma, a benign tumor derived from the hair apparatus and consisted of hair matrix cells, is relatively prevalent in females. We report a case of right preauricular calcifying epithelioma that was incidentally detected at the examination of multiple facial fractures and became an old lesion without symptoms for 40 years. The patient who was a 42-year-old male visited our department for the first time in October 2011 with a chief complaint of multiple facial fractures. Radiographic imaging demonstrated fracture lines at the anterior and posterior walls of the left maxillary sinus and zygomatic arch and revealed a mass at a right preauricular area. The extraction was performed under general anaesthesia. No recurrence has been observed 15 months after surgery. We also reviewed the literature of calcifying epithelioma.

  9. Clinical implications of calcifying nanoparticles in dental diseases: a critical review

    Directory of Open Access Journals (Sweden)

    Alenazy MS

    2013-12-01

    Full Text Available Mohammed S Alenazy,1 Hezekiah A Mosadomi2,3 1Restorative Dentistry Department, 2Oral and Maxillofacial Pathology Department, 3Research Center, Riyadh Colleges of Dentistry and Pharmacy, Riyadh, Saudi Arabia Background: Unknown cell-culture contaminants were described by Kajander and Ciftçioğlu in 1998. These contaminants were called nanobacteria initially and later calcifying nanoparticles (CNPs. Their exact nature is unclear and controversial. CNPs have unique and unusual characteristics, which preclude placing them into any established evolutionary branch of life. Aim: The aim of this systematic review was to assess published data concerning CNPs since 1998 in general and in relation to dental diseases in particular. Materials and methods: The National Library of Medicine (PubMed and Society of Photographic Instrumentation Engineers (SPIE electronic and manual searches were conducted. Nanobacteria and calcifying nanoparticles were used as keywords. The search yielded 135 full-length papers. Further screening of the titles and abstracts that followed the review criteria resulted in 43 papers that met the study aim. Conclusion: The review showed that the existence of nanobacteria is still controversial. Some investigators have described a possible involvement of CNPs in pulpal and salivary gland calcifications, as well as the possible therapeutic use of CNPs in the treatment of cracked and/or eroded teeth. Keywords: calcifying nanoparticles, nanobacteria, sialolith, pulp stone, enamel repair

  10. Calcifying odontogenic cyst of anterior maxilla with complex odontoma

    Directory of Open Access Journals (Sweden)

    Yadavalli Guruprasad

    2014-01-01

    Full Text Available Calcifying odontogenic cyst (COC is an unusual and unique lesion with characteristics of a solid neoplasm and of a cyst. It shows considerable amount of histopathological diversity, with variable clinical behavior such as cystic, neoplastic and infiltrating malignant behavior. It is a rare developmental odontogenic cyst with notable presence of histopathological features, which include a cystic lining demonstrating characteristic "Ghost" epithelial cells with a propensity to calcify. Odontomes are best known as hamartomatous benign tumors rather than true neoplasms, arising from odontogenic tissues. Histologically, they are classified as compound and complex variety. We report a rare case of COC of anterior maxilla with complex odontome in a 12-year-old female child.

  11. Atypical adult non-calcified pilomatricoma

    International Nuclear Information System (INIS)

    Masih, S.; Gentili, A.; Sorenson, S.M.; Seeger, L.L.

    2000-01-01

    We present a rare case of a non-calcified pilomatricoma in a 67-year-old man. This tumor was extremely large in size, and its location, in the lower extremity, was very unusual. The clinical, radiographic, and histopathological features are described in detail. The role of magnetic resonance imaging (MRI) in the diagnosis of this entity is discussed. Definite internal reticulations and septations were observed. A possible explanation for this observation is that the high signal intensity reticulations may represent edematous stroma surrounding basaloid cells. (orig.)

  12. Are calcifying matrix vesicles in atherosclerotic lesions of cellular origin?

    Science.gov (United States)

    Bobryshev, Yuri V; Killingsworth, Murray C; Huynh, Thuan G; Lord, Reginald S A; Grabs, Anthony J; Valenzuela, Stella M

    2007-03-01

    Over recent years, the role of matrix vesicles in the initial stages of arterial calcification has been recognized. Matrix calcifying vesicles have been isolated from atherosclerotic arteries and the biochemical composition of calcified vesicles has been studied. No studies have yet been carried out to examine the fine structure of matrix vesicles in order to visualize the features of the consequent stages of their calcification in arteries. In the present work, a high resolution ultrastructural analysis has been employed and the study revealed that matrix vesicles in human atherosclerotic lesions are heterogeneous with two main types which we classified. Type I calcified vesicles were presented by vesicles surrounded by two electron-dense layers and these vesicles were found to be resistant to the calcification process in atherosclerotic lesions in situ. Type II matrix vesicles were presented by vesicles surrounded by several electron-dense layers and these vesicles were found to represent calcifying vesicles in atherosclerotic lesions. To test the hypothesis that calcification of matrix vesicles surrounded by multilayer sheets may occur simply as a physicochemical process, independently from the cell regulation, we produced multilamellar liposomes and induced their calcification in vitro in a manner similar to that occurring in matrix vesicles in atherosclerotic lesions in situ.

  13. Different manifestations of calcifying cystic odontogenic tumor

    Directory of Open Access Journals (Sweden)

    Estevam Rubens Utumi

    2012-09-01

    Full Text Available The calcifying cystic odontogenic tumor normally presents as apainless, slow-growing mass, involving both maxilla and mandible,primarily the anterior segment (incisor/canine area. It generallyaffects young adults in the third to fourth decades, with no genderpredilection. Computerized tomography images revealed importantcharacteristics that were not detected by panoramic radiography,such as fenestration, calcification and tooth-like structures. Thetypical microscopic feature of this lesion is the presence of variableamounts of aberrant epithelial cells, without nuclei, which arenamed “ghost cells”. In addition, dysplastic dentine can be foundand occasionally the cyst can be associated with an area of dentalhard tissue formation resembling an odontoma. The treatment forcalcifying cystic odontogenic tumor involves simple enucleationand curettage. The purpose of this article is to present two differentmanifestation of calcifying cystic odontogenic tumor in whichcomputerized tomography, associated to clinical features, servedas an important tool for diagnosis, adequate surgical planning andfollow-up of patients.

  14. Manual Aspiration Thrombectomy in Patients with Acute Stroke-Related Calcified Cerebral Emboli.

    Science.gov (United States)

    Koh, Esther; Kwak, Hyo Sung; Chung, Gyung-Ho

    2017-10-01

    The aim of this study was to evaluate the effectiveness of mechanical aspiration thrombectomy (MAT) in patients with acute ischemic stroke from calcified cerebral emboli. Procedural results were reviewed for acute stroke patients with clinically neurological deficits who underwent recanalization from October 2012 through September 2015. Initial imaging studies and cerebral angiography were analyzed. Of the total number of patients with acute stroke, 5 patients were confirmed to have acute ischemic stroke by calcified cerebral emboli. On initial brain computed tomographic imaging, all patients showed small, dense single calcifications in the middle cerebral artery with no definitive ischemic low-density lesions (M1: 3, M2: 2, mean size: 4.8 mm). All patients had angiographic findings of filling defects from calcified emboli. Four patients had good collateral flow and two had continuous distal flow. All patients underwent MAT using a Penumbra catheter (Penumbra Inc., Alameda, CA). MAT did not remove calcified emboli in all patients. Two patients with good collateral flow had favorable functional outcomes (modified Rankin Scale score ≤2). Four patients had diffuse calcification in the aortic arch, carotid artery, and aortic valve. Cerebral angiography supports a diagnosis of stroke when calcified cerebral emboli have contrast-filling defects and a degree of vascular occlusion. However, in this study, MAT was not an effective treatment for patients with calcified cerebral emboli because of hardness of the calcified plaque and packing into the arterial lumen. Copyright © 2017 National Stroke Association. Published by Elsevier Inc. All rights reserved.

  15. Targeting angiogenesis-dependent calcified neoplasms using combined polymer therapeutics.

    Directory of Open Access Journals (Sweden)

    Ehud Segal

    Full Text Available There is an immense clinical need for novel therapeutics for the treatment of angiogenesis-dependent calcified neoplasms such as osteosarcomas and bone metastases. We developed a new therapeutic strategy to target bone metastases and calcified neoplasms using combined polymer-bound angiogenesis inhibitors. Using an advanced "living polymerization" technique, the reversible addition-fragmentation chain transfer (RAFT, we conjugated the aminobisphosphonate alendronate (ALN, and the potent anti-angiogenic agent TNP-470 with N-(2-hydroxypropylmethacrylamide (HPMA copolymer through a Glycine-Glycine-Proline-Norleucine linker, cleaved by cathepsin K, a cysteine protease overexpressed at resorption sites in bone tissues. In this approach, dual targeting is achieved. Passive accumulation is possible due to the increase in molecular weight following polymer conjugation of the drugs, thus extravasating from the tumor leaky vessels and not from normal healthy vessels. Active targeting to the calcified tissues is achieved by ALN's affinity to bone mineral.The anti-angiogenic and antitumor potency of HPMA copolymer-ALN-TNP-470 conjugate was evaluated both in vitro and in vivo. We show that free and conjugated ALN-TNP-470 have synergistic anti-angiogenic and antitumor activity by inhibiting proliferation, migration and capillary-like tube formation of endothelial and human osteosarcoma cells in vitro. Evaluation of anti-angiogenic, antitumor activity and body distribution of HPMA copolymer-ALN-TNP-470 conjugate was performed on severe combined immunodeficiency (SCID male mice inoculated with mCherry-labeled MG-63-Ras human osteosarcoma and by modified Miles permeability assay. Our targeted bi-specific conjugate reduced VEGF-induced vascular hyperpermeability by 92% and remarkably inhibited osteosarcoma growth in mice by 96%.This is the first report to describe a new concept of a narrowly-dispersed combined polymer therapeutic designed to target both tumor and

  16. In vitro electromagnetically stimulated SAOS-2 osteoblasts inside porous hydroxyapatite

    Science.gov (United States)

    Fassina, Lorenzo; Saino, Enrica; Sbarra, Maria Sonia; Visai, Livia; De Angelis, Maria Gabriella Cusella; Magenes, Giovanni; Benazzo, Francesco

    2009-01-01

    One of the key challenges in reconstructive bone surgery is to provide living constructs that possess the ability to integrate in the surrounding tissue. Bone graft substitutes, such as autografts, allografts, xenografts, and biomaterials have been widely used to heal critical-size long bone defects due to trauma, tumor resection, congenital deformity, and tissue degeneration. In particular, porous hydroxyapatite is widely used in reconstructive bone surgery owing to its biocompatibility. In addition, the in vitro modification of hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix inside a porous hydroxyapatite scaffold. The electromagnetic stimulus had the following parameters: intensity of the magnetic field equal to 2 mT, amplitude of the induced electric tension equal to 5 mV, frequency of 75 Hz, and pulse duration of 1.3 ms. In comparison with control conditions, the electromagnetic stimulus increased the cell proliferation and the surface coating with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The physical stimulus aimed at obtaining a better modification of the biomaterial internal surface in terms of cell colonization and coating with bone matrix. PMID:19827111

  17. The Monitoring Of Thunderstorm In Sao Paulo's Urban Areas, Brazil

    Science.gov (United States)

    Gin, R. B.; Pereira, A.; Beneti, C.; Jusevicius, M.; Kawano, M.; Bianchi, R.; Bellodi, M.

    2005-12-01

    A monitoring of thunderstorm in urban areas occurred in the vicinity of Sao Bernardo do Campo, Sao Paulo from November 2004 to March 2005. Eight thunderstorms were monitored by local electric field, video camera, Brazilian Lightning Location Network (RINDAT) and weather radar. The most of these thunderstorms were associated with the local convection and cold front. Some of these events presented floods in the vicinity of Sao Bernardo and in the Metropolitan Area of Sao Paulo (MASP) being associated with local sea breeze circulation and the heat island effect. The convectives cells exceeding 100km x 100 km of area, actives between 2 and 3 hours. The local electric field identified the electrification stage of thunderstorms, high transients of lightning and total lightning rate of above 10 flashes per minute. About 29.5 thousands of cloud-to-ground lightning flashes were analyzed . From the total set of CG flashes analyzed, about 94 percent were negative strokes and presented average peak current of above 25kA, common for this region. Some lightning images were obtained by video camera and compared with transients of lightning and lightning detection network data. The most of these transients of lightning presented continuing current duration between 100ms and 200ms. A CG lightning occurred on 25th February was visually observed 3.5km from FEI campus, Sao Bernardo do Campo. This lightning presented negative polarity and estimed peak current of above 30kA. A spider was visually observed over FEI Campus at 17th March. No transients of lightning and recording by lightning location network were found.

  18. Metal concentration in urban park soils of Sao Paulo 2. Buenos AiresPark

    International Nuclear Information System (INIS)

    Gumiero, Felipe C.; Figueiredo, Ana Maria G.; Camargo, Sonia P.; Pavese, Arthur; Sigolo, Joel B.

    2007-01-01

    As part of a project which aims metal concentration assessment in urban park soils of Sao Paulo, in the present paper the concentration of the elements As, Ba, Cr, Co, Cu, Pb, Sb and Zn were determined in surface soil samples (0-5 cm and 0-20 cm) from Buenos Aires park of Sao Paulo. This park is located in central region of the city, and is surrounded by avenues and streets, with different traffic volumes. Instrumental Neutron Activation Analysis (INAA) and X-ray Fluorescence (FRX) were used for metal analysis. Preliminary results showed concentration levels of the analyzed elements higher than the values considered as reference values for soils in Sao Paulo, according to the Environmental Protection Agency of the State of Sao Paulo (CETESB). These results suggest that these elements have anthropogenic origin and indicate a potential risk for soil quality. (author)

  19. Giant, Completely Calcified Lumbar Juxtafacet Cyst: Report of an Unusual Case

    Science.gov (United States)

    Huang, Kevin T.; Owens, Timothy R.; Wang, Teresa S.; Moreno, Jessica R.; Bagley, Jacob H.; Bagley, Carlos A.

    2013-01-01

    Study Design Case report. Objective To report the case of one patient who developed a giant, completely calcified, juxtafacet cyst. Methods A 57-year-old woman presented with a 2-year history of progressively worsening lower back pain, left leg pain, weakness, and paresthesias. Imaging showed a giant, completely calcified mass arising from the left L5–S1 facet joint, with coexisting grade I L5 on S1 anterolisthesis. The patient was treated with laminectomy, excision of the mass, and L5–S1 fixation and fusion. Results The patient had an uncomplicated postoperative course and had complete resolution of her symptoms as of 1-year follow-up. Conclusions When presented with a solid-appearing, calcified mass arising from the facet joint, a completely calcified juxtafacet cyst should be considered as part of the differential diagnosis. PMID:25083359

  20. Live tissue imaging shows reef corals elevate pH under their calcifying tissue relative to seawater.

    Directory of Open Access Journals (Sweden)

    Alexander Venn

    Full Text Available The threat posed to coral reefs by changes in seawater pH and carbonate chemistry (ocean acidification raises the need for a better mechanistic understanding of physiological processes linked to coral calcification. Current models of coral calcification argue that corals elevate extracellular pH under their calcifying tissue relative to seawater to promote skeleton formation, but pH measurements taken from the calcifying tissue of living, intact corals have not been achieved to date. We performed live tissue imaging of the reef coral Stylophora pistillata to determine extracellular pH under the calcifying tissue and intracellular pH in calicoblastic cells. We worked with actively calcifying corals under flowing seawater and show that extracellular pH (pHe under the calicoblastic epithelium is elevated by ∼0.5 and ∼0.2 pH units relative to the surrounding seawater in light and dark conditions respectively. By contrast, the intracellular pH (pHi of the calicoblastic epithelium remains stable in the light and dark. Estimates of aragonite saturation states derived from our data indicate the elevation in subcalicoblastic pHe favour calcification and may thus be a critical step in the calcification process. However, the observed close association of the calicoblastic epithelium with the underlying crystals suggests that the calicoblastic cells influence the growth of the coral skeleton by other processes in addition to pHe modification. The procedure used in the current study provides a novel, tangible approach for future investigations into these processes and the impact of environmental change on the cellular mechanisms underpinning coral calcification.

  1. Stimulation by parathyroid hormone of sup 45 Ca sup 2+ uptake in osteoblast-like cells: Possible involvement of alkaline phosphatase

    Energy Technology Data Exchange (ETDEWEB)

    Fukayama, S.; Tashjian, A.H. Jr. (Harvard School of Public Health, Boston, MA (USA))

    1990-04-01

    We have investigated the actions of human PTH (hPTH-(1-34)) on the association of 45Ca2+ with two human (SaOS-2 and MG-63) and two rat (ROS 17/2.8 and UMR-106) osteoblast-like cell types. In SaOS-2 cells, hPTH-(1-34) binds to specific membrane receptors to activate adenylate cyclase. Treatment of SaOS-2 cells with hPTH-(1-34) resulted in an increase in 45Ca2+ uptake, in a dose-dependent fashion, up to 2- to 4-fold above control values. The increase was first evident at 10 min and persisted for at least 30 min. Treatment with nimodipine, a calcium channel antagonist, was without effect on the stimulatory action of PTH. A similar enhancement of cell-associated 45Ca2+ was observed when the cells were incubated with vasoactive intestinal peptide, which acts via different receptors to activate adenylate cyclase in SaOS-2 cells. Treatment with (Bu)2cAMP also induced an increase in cell-associated 45Ca2+. Pretreatment of SaOS-2 cells with hPTH-(1-34) for 4 h, which induced homologous desensitization to a second challenge with the same peptide for stimulation of cAMP production, did not attenuate the further enhancement of cell-associated 45Ca2+ by a second treatment with hPTH-(1-34). We then examined a possible relationship between alkaline phosphatase (ALPase) and 45Ca2+ uptake. SaOS-2 cells contained high levels of alkaline phosphatase activity and continuously released the enzyme into the medium. Release was enhanced by treatment with hPTH-(1-34) for 10 min. Incubation of cells with levamisole (an inhibitor of the liver/bone/kidney type of ALPase) resulted in a rapid decrease in basal and PTH-stimulated 45Ca2+ uptake, while treatment with L-Phe-Gly-Gly was without effect. Treatment of the cells with ALPase (bovine kidney) enhanced 45Ca2+ uptake. In MG-63 cells, a stimulatory effect of hPTH-(1-34) on cell-associated 45Ca2+ was also observed; however, hPTH-(1-34) did not stimulate cAMP production in MG-63 cells.

  2. Calcified pulmonary thromboembolism in a child with sickle cell disease: value of multidetector CT in patients with acute chest syndrome

    International Nuclear Information System (INIS)

    Staser, Jonathan A.; Alam, Tariq; Applegate, Kimberly

    2006-01-01

    The incidence of pulmonary embolism in children is not clearly known, but is believed to be low. Risk factors for pulmonary thromboembolism include central venous catheter, malignancy, surgery, infection, trauma, and congenital hypercoagulable disorders. Children with sickle cell disease are prothrombotic and are at an increased risk of thromboembolism. The incidence of this event is unknown because these children are often not thoroughly imaged. We report here a case of a calcified pulmonary thromboembolism in a child with sickle cell disease and emphasize the use of multidetector CT in detection of pulmonary thromboembolism in children with sickle cell disease. (orig.)

  3. Calcified subdural hematoma associated with hypertensive intracerebral hemorrhage

    International Nuclear Information System (INIS)

    Ishige, Naoki; Sunami, Kenro; Sato, Akira; Watanabe, Osamu

    1984-01-01

    A case of calcified subdural hematoma associated with hypertensive intracerebral hemorrhage is reported. A left frontal subdural hematoma with left putaminal hemorrhage was incidentally found when a CT scan was performed to evaluate right hemiparesis and aphasia in a 55-year-old man. The putaminal hemorrhage was not very extensive, but his clinical symptoms were rather serious. Not only the putaminal hemorrhage, but also the presence of the calcified subdural hematoma was considered to have caused his clinical deterioration. The subtotal removal of the calcified subdural hematoma brought about a good result. (author)

  4. Sudden Unilateral Vision Loss Arising from Calcified Amorphous Tumor of the Left Ventricle

    Science.gov (United States)

    Nazli, Yunus; Colak, Necmettin; Atar, Inci Asli; Alpay, Mehmet Fatih; Haltas, Hacer; Eryonucu, Beyhan; Cakir, Omer

    2013-01-01

    Calcified amorphous tumor of the heart is a very rare non-neoplastic intracavitary mass. The clinical presentation is similar to that of other cardiac masses. The precise cause and best approach to treatment remain unclear. We describe a case of cardiac calcified amorphous tumor presenting with refractory unilateral vision loss that was successfully treated by surgical excision. To our knowledge, this is only the 2nd reported case of retinal arterial embolism due to cardiac calcified amorphous tumor in the English-language literature. PMID:24082378

  5. Calcifying tendinitis of the rotator cuff with cortical bone erosion

    Energy Technology Data Exchange (ETDEWEB)

    Chan, Roxanne; Kim, David H.; Millett, Peter J. [Harvard Medical School, Brigham and Women' s Hospital, Boston, Massachusetts (United States); Weissman, Barbara N. [Harvard Medical School, Brigham and Women' s Hospital, Boston, Massachusetts (United States); Brigham and Women' s Hospital, Department of Radiology, Musculoskeletal Division, Boston (United States)

    2004-10-01

    Calcifying tendinitis occurs most commonly in the rotator cuff tendons, particularly involving the supraspinatus tendon insertion, and is often asymptomatic. Cortical erosion secondary to calcifying tendinitis has been reported in multiple locations, including in the rotator cuff tendons. We present a pathologically proven case of symptomatic calcifying tendinitis involving the infraspinatus tendon with cortical erosion with correlative radiographic, CT, and MR findings. The importance of considering this diagnosis when evaluating lytic lesions of the humerus and the imaging differential diagnosis of calcifying tendinitis and cortical erosion are discussed. (orig.)

  6. Calcifying tendinitis of the rotator cuff with cortical bone erosion

    International Nuclear Information System (INIS)

    Chan, Roxanne; Kim, David H.; Millett, Peter J.; Weissman, Barbara N.

    2004-01-01

    Calcifying tendinitis occurs most commonly in the rotator cuff tendons, particularly involving the supraspinatus tendon insertion, and is often asymptomatic. Cortical erosion secondary to calcifying tendinitis has been reported in multiple locations, including in the rotator cuff tendons. We present a pathologically proven case of symptomatic calcifying tendinitis involving the infraspinatus tendon with cortical erosion with correlative radiographic, CT, and MR findings. The importance of considering this diagnosis when evaluating lytic lesions of the humerus and the imaging differential diagnosis of calcifying tendinitis and cortical erosion are discussed. (orig.)

  7. Odonto calcifying cyst

    Directory of Open Access Journals (Sweden)

    Nalini Aswath

    2013-01-01

    Full Text Available The calcifying odontogenic cyst (COC is reported to be associated with odontoma in 24% of cases. Separation of the cases of calcifying odontogenic cyst associated with odontoma (COCaO may lead to a better understanding of the pathogenesis of this lesion. The literature revealed 52 cases of COCaO. The male to female ratio was 1:1.9, with a mean age of 16 years. Most common location was the maxilla (61.5%. The radiographic appearance of most cases (80.5% was a well-defined, mixed radiolucent-radiopaque lesion. Histologically, the lesions consisted of a single large cyst with tooth-like structures as an integral part, giving the impression of a single lesion. In addition to the unique histologic features, differences in gender and distribution were found between the cases of COCaO and those of simple COC. COCaO may be regarded as a separate entity and classified as a benign, mixed odontogenic tumor. The term odontocalcifying odontogenic cyst is suggested.

  8. Odonto calcifying cyst.

    Science.gov (United States)

    Aswath, Nalini; Mastan, Kader; Manikandan, Tirupathi; Samuel, Gigi

    2013-01-01

    The calcifying odontogenic cyst (COC) is reported to be associated with odontoma in 24% of cases. Separation of the cases of calcifying odontogenic cyst associated with odontoma (COCaO) may lead to a better understanding of the pathogenesis of this lesion. The literature revealed 52 cases of COCaO. The male to female ratio was 1:1.9, with a mean age of 16 years. Most common location was the maxilla (61.5%). The radiographic appearance of most cases (80.5%) was a well-defined, mixed radiolucent-radiopaque lesion. Histologically, the lesions consisted of a single large cyst with tooth-like structures as an integral part, giving the impression of a single lesion. In addition to the unique histologic features, differences in gender and distribution were found between the cases of COCaO and those of simple COC. COCaO may be regarded as a separate entity and classified as a benign, mixed odontogenic tumor. The term odontocalcifying odontogenic cyst is suggested.

  9. Impact of glucose intolerance on coronary calcified lesions evaluated using multislice computed tomography

    International Nuclear Information System (INIS)

    Nomura, Kyoko; Ohwaki, Kazuhiro; Yano, Eiji; Yamanouchi, Toshikazu; Kim, Gwang U.

    2007-01-01

    Metabolic syndrome has the unique concept that the common occurrence of individual disease components increases the risk of coronary artery disease (CAD). However, some studies suggest that the burden of different CAD risk factors is not equal, and focusing on the whole set of risk factors might neglect the impact of individual factors that could be useful targets for prophylactic therapies. The purpose of this study was to investigate the effect of glucose intolerance on CAD using multislice computed tomography (MSCT). Ninety-eight consecutive patients with at least one traditional CAD risk factor who visited a municipal hospital were enrolled in this study. The risk factors were impaired glucose tolerance (fasting glucose≥110 mg/dl or patients with diabetes), low high-density lipoprotein cholesterol (HDL-C, 25 kg/m 2 for men and >23 kg/m 2 for women). CAD was determined by the presence of either stenoses, non-calcified plaques or calcified lesions. The following risk factors were significantly related in univariate logistic models: glucose intolerance and coronary calcified lesions (p=0.001), and hypertriglycemia and non-calcified plaque lesions (p=0.048). Multivariate models showed that glucose intolerance was significantly associated with calcified lesions, even after adjustment for gender, age, low HDL-C, hypertriglycemia, hypertension, and obesity (p=0.018). Our results suggest that glucose intolerance might be closely related to the presence of coronary calcified lesions among traditional CAD risk factors. (author)

  10. The basic and ultrabasic dikes from the coast region between the Sao Sebastiao and Ubatuba cities, Sao Paulo State, SP, Brazil; Os diques basicos e ultrabasicos da regiao costeira entre as cidades de Sao Sebastiao e Ubatuba, estado de Sao Paulo, SP, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Garda, Gianna Maria

    1995-12-31

    The coastline between Sao Sebastiao and Ubatuba cities and the shores of Sao Sebastiao, Anchieta and Mar Virado islands (Sao Paulo State, Brazil) are crosscut by several small swarms and isolated dykes trending N55E. The main rock types range from basic to intermediate, but also a conspicuous variety of alkaline lamprophyres occur side by side with the main group. The thickness of the basic to intermediate dykes vary widely, from a few centimeters to several metres, while the lamprophyres are a few tens of centimeters thick. The objective of this thesis is the petrographic, mineralogic, petrochemical and isotopic characterization of the basic and ultrabasic dykes that occur between the Sao Sebastiao and Ubatuba cities (State of Sao Paulo), also including some occurrences from the Sao Sebastiao, Mar Virado and Anchieta islands and from the Bairro Alto region (Folha de Natividade da Serra). The petrogenetic model presented is based in the national and international bibliography. (author) 146 refs., 44 figs., 24 tabs.

  11. The basic and ultrabasic dikes from the coast region between the Sao Sebastiao and Ubatuba cities, Sao Paulo State, SP, Brazil; Os diques basicos e ultrabasicos da regiao costeira entre as cidades de Sao Sebastiao e Ubatuba, estado de Sao Paulo, SP, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Garda, Gianna Maria

    1996-12-31

    The coastline between Sao Sebastiao and Ubatuba cities and the shores of Sao Sebastiao, Anchieta and Mar Virado islands (Sao Paulo State, Brazil) are crosscut by several small swarms and isolated dykes trending N55E. The main rock types range from basic to intermediate, but also a conspicuous variety of alkaline lamprophyres occur side by side with the main group. The thickness of the basic to intermediate dykes vary widely, from a few centimeters to several metres, while the lamprophyres are a few tens of centimeters thick. The objective of this thesis is the petrographic, mineralogic, petrochemical and isotopic characterization of the basic and ultrabasic dykes that occur between the Sao Sebastiao and Ubatuba cities (State of Sao Paulo), also including some occurrences from the Sao Sebastiao, Mar Virado and Anchieta islands and from the Bairro Alto region (Folha de Natividade da Serra). The petrogenetic model presented is based in the national and international bibliography. (author) 146 refs., 44 figs., 24 tabs.

  12. Supraspinatus Intramuscular Calcified Hematoma or Necrosis Associated with Tendon Tear

    Directory of Open Access Journals (Sweden)

    Alexandre Lädermann

    2015-01-01

    Full Text Available Introduction. Rotator cuff intramuscular calcification is a rare condition usually caused by heterotopic ossification and myositis ossificans. Case Presentation. We describe a patient with voluminous calcified mass entrapped in supraspinatus muscle associated with corresponding tendon tear. Histological examination corresponded to a calcified hematoma or necrosis. Patient was surgically managed with open excision of the calcified hematoma and rotator cuff arthroscopic repair. At 6 months, supraspinatus muscle was healed, and functional outcome was good. Discussion and Conclusion. We hypothesized that supraspinatus intramuscular calcified hematoma was responsible for mechanical stress on the tendon. This association has never been described.

  13. Calcified Mass on Brain CT in a Teenager with Refractory Seizures.

    Science.gov (United States)

    Khalatbari, Mahmoud Reza; Brunetti, Enrico; Shobeiri, Elham; Moharamzad, Yashar

    2014-12-01

    Cerebral echinococcosis is very rare, representing 2% of all cystic echinococcosis (CE) cases. Primary echinococcal cysts of the brain are extremely rare in pediatric patients. We report on a 16-year-old boy referred to our tertiary center with intractable epilepsy for the previous three years despite receiving full doses of three antiepileptic medications. Brain computed tomography (CT) showed a left frontal calcified mass. Magnetic resonance imaging (MRI) of the brain revealed a well-defined spherical mass in the left frontal lobe, slightly hypointense on T1-weighted and heterogeneous hyperintense on T2-weighted images with no contrast enhancement. With a broad differential list in mind, a surgical intervention was planned. During surgery, a primary calcified cerebral echinococcal cyst with severe adhesion to the adjacent dura of the frontal region was discovered and removed intact. Histopathology examination confirmed the diagnosis. Only phenobarbital was continued and no medical therapy for CE was administered. Two years after surgery, the patient remained free of seizures. In areas endemic for CE, cerebral echinococcal cyst should be included in the differential list of patients with intractable seizures. Though rare, this entity can present itself as a calcified mass on neuroimaging. Surgical removal of the calcified cyst is necessary for control and treatment of the epilepsy.

  14. Utilidad de la relación Sao2/Fio2 en la evaluación del grado de compromiso pulmonar en pacientes críticos

    Directory of Open Access Journals (Sweden)

    Alonso Gómez Duque

    2002-01-01

    Full Text Available Background: Arteriovenous admixture or intrapulmonary shunt and the relation between PaO2 and FI02 (PaO2/FiO2 have been advocated as the main indicators of pulmonary function. Because the Pa02 is a measure of the oxygen given to the tissues, as well as the Sa02, we could expect a close relationship between the Pa02IFI02 index and a new one, the SaO2/FiO2 index, Methods: We conducted a prospective study in a cohortofl07 patients with different pathologies in an Intensive Care Unit, collecting 507 samples of arterial and central venous gases and measuring the Sa02 by pulse oximeter at the same time. PaO2/FiO2 and Sa O2/FiO2, as well as intrapulmonary shunt using invasive and noninvasive methods, were caIculated. The Lung Injury Score (LIS and the Multiple Organ Dysfunction score were calculated too. Results: We found a good correlation between Sa O2/FiO2 and PaO2/FiO2. (R2=0,81,R=0.9, and between the intrapulmonary shuntfraction calculated with invasive and noninvasive methods.(R2=0.75, R=0.86. The data were correlationed with a simple linear regression analysis. Five groups of SaO2/FiO2 were found and a punctuation from Oto 4 was established for them, in a similar approach to the one used by Murray in the Lung Injury Score (LIS and Marshall in the Multiple Organ Dysfunction score. By using this punctuation, we replaced the PaO2/FiO2 by the SaO2/FiO2 in the scale and recalculated the LIS finding a good correlation between the score with PaO2/FiO2 and the one with SaO2/FiO2 (R2=0.94,R=0.96. The same was true for the correlation between the Marshall score with PaO2/FiO2 and the one with SaO2/FiO2. (R2=O.85, R=0.92. The correlation was mantained when a stratified analysis was made according to the degree of severity of the pulmonary injury. Conclusions: SaO2/FiO2 is an useful indicator of the oxygenation in a similar way as the PaO2/FiO2 índex, It could be incorporated into the LIS and Marshall score with similar results. The usefulness of the

  15. Calcifying epithelial odontogenic cyst with odontome in left mandible

    Directory of Open Access Journals (Sweden)

    R Kamala

    2009-01-01

    Full Text Available Calcifying epithelial odontogenic cyst is a mixed odontogenic benign tumor but most of the cases present cystic characteristics. We present a case report of calcifying epithelial odontogenic cyst with odontoma in a 20-year-old male who presented with a painless unilateral swelling of the jaw. The lesion involved an impacted 37 which was displaced to the lower border of the mandible and a calcified mass that was within the cystic lesion that was recognized as odontoma. The lesion was surgically removed along with 36 and 37 was allowed to erupt.

  16. Basic and ultrabasic coastal dykes adjacent to the Sao Sebastiao Channel (North coast of Sao Paulo State, Brazil)

    International Nuclear Information System (INIS)

    Garda, Gianna Maria; Schorscher, Johann Hans Daniel

    1996-01-01

    The portions of the Precambrian Costeiro Complex adjacent to the Sao Sebastiao Channel (Sao Paulo State, Brazil) are crosscut by mafic dykes of basic to intermediate composition, lamprophyres and trachytes. The former have been correlated with the basalts of the Parana Basin, while the lamprophyres are expressions of an alkaline magmatism different from the one that happened approximately 80 Ma ago in the Sao Sebastiao, Buzios, Vitoria and Montao de Trigo islands, where more acid terms predominate with associated trachytes and rare phonolites. The basic-ultrabasic lamprophyric magmatism is widespread along the Sao Paulo and Rio de Janeiro coasts; they are independent from and most likely older than the stocks and alkaline massifs. (author)

  17. The basic and ultrabasic dikes from the coast region between the Sao Sebastiao and Ubatuba cities, Sao Paulo State, SP, Brazil

    International Nuclear Information System (INIS)

    Garda, Gianna Maria

    1995-01-01

    The coastline between Sao Sebastiao and Ubatuba cities and the shores of Sao Sebastiao, Anchieta and Mar Virado islands (Sao Paulo State, Brazil) are crosscut by several small swarms and isolated dykes trending N55E. The main rock types range from basic to intermediate, but also a conspicuous variety of alkaline lamprophyres occur side by side with the main group. The thickness of the basic to intermediate dykes vary widely, from a few centimeters to several metres, while the lamprophyres are a few tens of centimeters thick. The objective of this thesis is the petrographic, mineralogic, petrochemical and isotopic characterization of the basic and ultrabasic dykes that occur between the Sao Sebastiao and Ubatuba cities (State of Sao Paulo), also including some occurrences from the Sao Sebastiao, Mar Virado and Anchieta islands and from the Bairro Alto region (Folha de Natividade da Serra). The petrogenetic model presented is based in the national and international bibliography. (author)

  18. Geochronological study of the metaconglomerates of the Sao Roque Group, Sao Paulo State

    International Nuclear Information System (INIS)

    Tassinari, C.C.G.; Kawashita, K.; Kikuchi, R.K.P. de

    1985-01-01

    This work deals with the geochronological study of the metaconglomerates of the Sao Roque Group by Rb-Sr whole rock measurements and K-Ar method in minerals concentrate. The granite-gneissic pebbles from the metaconglomerates have been analysed by Rb-Sr isochronic method and yelded an age of 1200+100 Ma. with a 87 Sr/ 86 Sr intercept of 0.737 +- 0.003. In our view this age might be associated to the metamorphic episode affecting the pre-existent granitic rocks before the epoch of the Sao Roque group sedimentation, or to the original age of the pebbles. The analytical points from the conglomerate matrix seem to define a linear array with 800Ma. This age, probably, represents the epoch of the main metamorphic event on the Sao Roque Group. The K-Ar micas determinations on rocks of the unit in study are concordant in ages, with values around 620.. These ages represent the tectonic estabilization epoch of the Pico de Jaragua region. (Author) [pt

  19. [Calcifying tendinitis of the rotator cuff with focal umeral osteolysis. Imaging features].

    Science.gov (United States)

    Mascarenhas, V V; Morais, F; Marques, H; Guerra, A; Carpinteiro, E; Gaspar, A

    2015-01-01

    Calcifying tendinitis occurs most commonly in the rotator cuff tendons, particularly involving the supraspinatus tendon insertion, and is often asymptomatic. Cortical erosion secondary to calcifying tendinitis has been reported in multiple locations, including in the rotator cuff tendons. The authors report two cases of symptomatic calcifying tendinitis involving the infraspinatus tendon with cortical erosion with correlative radiographic, and MR findings. The importance of considering this diagnosis when evaluating lytic lesions of the humerus and the imaging differential diagnosis of calcifying tendinitis and cortical erosion are discussed.

  20. Progression from calcifying tendinitis to rotator cuff tear

    Energy Technology Data Exchange (ETDEWEB)

    Gotoh, Masafumi; Higuchi, Fujio; Suzuki, Ritsu; Yamanaka, Kensuke [Department of Orthopaedic Surgery, Medical Center of Kurume University, 155-1 Kokubu-machi, Kurume City, Fukuoka 839-0862 (Japan)

    2003-02-01

    This report documents the clinical, radiographic and histologic findings in a 46-year-old man with calcifying tendinitis in his left shoulder which progressed to rotator cuff tear. The patient had a 1-year history of repeated calcifying tendinitis before being referred to our hospital. On the initial visit, radiographs and magnetic resonance imaging (MRI) revealed calcium deposition localized in the supraspinatus tendon without apparent tear. Three months after the first visit, MRI revealed a partial-thickness rotator cuff tear at the site of calcium deposition. Surgical and histologic findings demonstrated that calcium deposition was the cause of cuff rupture. To our knowledge, based on a review of the English literature, this is the first case report in which the progression from calcifying tendinitis to rotator cuff tear has been serially observed. (orig.)

  1. Progression from calcifying tendinitis to rotator cuff tear

    International Nuclear Information System (INIS)

    Gotoh, Masafumi; Higuchi, Fujio; Suzuki, Ritsu; Yamanaka, Kensuke

    2003-01-01

    This report documents the clinical, radiographic and histologic findings in a 46-year-old man with calcifying tendinitis in his left shoulder which progressed to rotator cuff tear. The patient had a 1-year history of repeated calcifying tendinitis before being referred to our hospital. On the initial visit, radiographs and magnetic resonance imaging (MRI) revealed calcium deposition localized in the supraspinatus tendon without apparent tear. Three months after the first visit, MRI revealed a partial-thickness rotator cuff tear at the site of calcium deposition. Surgical and histologic findings demonstrated that calcium deposition was the cause of cuff rupture. To our knowledge, based on a review of the English literature, this is the first case report in which the progression from calcifying tendinitis to rotator cuff tear has been serially observed. (orig.)

  2. Calcifying tendinitis of the rotator cuff with focal umeral osteolysis. Imaging features

    Directory of Open Access Journals (Sweden)

    V. V. Mascarenhas

    2015-10-01

    Full Text Available Calcifying tendinitis occurs most commonly in the rotator cuff tendons, particularly involving the supraspinatus tendon insertion, and is often asymptomatic. Cortical erosion secondary to calcifying tendinitis has been reported in multiple locations, including in the rotator cuff tendons. The authors report two cases of symptomatic calcifying tendinitis involving the infraspinatus tendon with cortical erosion with correlative radiographic, and MR findings. The importance of considering this diagnosis when evaluating lytic lesions of the humerus and the imaging differential diagnosis of calcifying tendinitis and cortical erosion are discussed.

  3. TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast-like cells in a size dependent manner.

    Science.gov (United States)

    Ibrahim, Mohamed; Schoelermann, Julia; Mustafa, Kamal; Cimpan, Mihaela R

    2018-04-30

    Human exposure to titanium dioxide nanoparticles (nano-TiO 2 ) is increasing. An internal source of nano-TiO 2 is represented by titanium-based orthopedic and dental implants can release nanoparticles (NPs) upon abrasion. Little is known about how the size of NPs influences their interaction with cytoskeletal protein networks and the functional/homeostatic consequences that might follow at the implant-bone interface with regard to osteoblasts. We investigated the effects of size of anatase nano-TiO 2 on SaOS-2 human osteoblast-like cells exposed to clinically relevant concentrations (0.05, 0.5, 5 mg/L) of 5 and 40 nm spherical nano-TiO 2 . Cell viability and proliferation, adhesion, spread and migration were assessed, as well as the orientation of actin and microtubule cytoskeletal networks. The phosphorylation of focal adhesion kinase (p-FAK Y397 ) and the expression of vinculin in response to nano-TiO 2 were also assessed. Treatment with nano-TiO 2 disrupted the actin and microtubule cytoskeletal networks leading to morphological modifications of SaOS-2 cells. The phosphorylation of p-FAK Y397 and the expression of vinculin were also modified depending on the particle size, which affected cell adhesion. Consequently, the cell migration was significantly impaired in the 5 nm-exposed cells compared to unexposed cells. The present work shows that the orientation of cytoskeletal networks and the focal adhesion proteins and subsequently the adhesion, spread and migration of SaOS-2 cells were affected by the selected nano-TiO 2 in a size dependent manner. This article is protected by copyright. All rights reserved. © 2018 Wiley Periodicals, Inc.

  4. Calcified epidural hematoma in pediatric age group: A report of two cases

    Directory of Open Access Journals (Sweden)

    A Trivedi

    2010-01-01

    Full Text Available The authors present a rare case of calcified (ossified chronic epidural hematoma developed in a six-and-a-half-year-old female patient who was operated for cerebellar astrocytoma 6 months earlier. There was no history of trauma. Ossified epidural hematoma was seen as an incidental finding in the follow-up in computed tomography scan after 6 months of primary glioma surgery. Ossified chronic epidural hematoma with thick collagenous wall and newly formed bone on dura was excised. The development of calcified chronic subdural hematoma after decompressive intracranial surgery is a well-known occurrence, but the fact that a calcified epidural hematoma, which is rare and which can also develop after decompressive surgery, and the occurrence of calcified (ossified epidural hematoma after postfossa a glioma surgery is not yet reported. The second case is a 9-year-old male anemic child with a history of fall while playing 5 months earlier who presented with headache of 3 months duration. He had bifrontal calcified epidural hematoma operated by craniotomy and excision of calcified dural edge.

  5. Calcifying Bursitis ischioglutealis: A Case report

    Science.gov (United States)

    Schuh, Alexander; Narayan, Chirag Thonse; Schuh, Ralph; Hönle, Wolfgang

    2011-01-01

    Introduction: The ischiogluteal bursa is an inconstant anatomical finding located between the ischial tuberosity and the gluteus maximus. Ischiogluteal bursitis is a rare disorder. Case Report: We report the case of a 43-year-old female patient with bilateral calcifying ischiogluteal bursitis. The patient had no relevant medical history of systemic illness or major trauma to the buttock. After aspiration of both ischiogluteal bursitis which delievered calcareous deposits and instillation of a mixture of 1cc betamethasone (6 mg) and 4 cc of 1% lidocaine the patient was out of any complaints. Conclusion: Calcifying ischiogluteal bursitis is a rare entity but easily diagnosed on radiographs. Aspiration and local steroid instillation give good relief from symptoms. PMID:27298836

  6. Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy

    Directory of Open Access Journals (Sweden)

    T. M. DeCarlo

    2017-11-01

    Full Text Available Quantifying the saturation state of aragonite (ΩAr within the calcifying fluid of corals is critical for understanding their biomineralization process and sensitivity to environmental changes including ocean acidification. Recent advances in microscopy, microprobes, and isotope geochemistry enable the determination of calcifying fluid pH and [CO32−], but direct quantification of ΩAr (where ΩAr =  [CO32−][Ca2+]∕Ksp has proved elusive. Here we test a new technique for deriving ΩAr based on Raman spectroscopy. First, we analysed abiogenic aragonite crystals precipitated under a range of ΩAr from 10 to 34, and we found a strong dependence of Raman peak width on ΩAr with no significant effects of other factors including pH, Mg∕Ca partitioning, and temperature. Validation of our Raman technique for corals is difficult because there are presently no direct measurements of calcifying fluid ΩAr available for comparison. However, Raman analysis of the international coral standard JCp-1 produced ΩAr of 12.3 ± 0.3, which we demonstrate is consistent with published skeletal Mg∕Ca, Sr∕Ca, B∕Ca, δ11B, and δ44Ca data. Raman measurements are rapid ( ≤  1 s, high-resolution ( ≤  1 µm, precise (derived ΩAr ± 1 to 2 per spectrum depending on instrument configuration, accurate ( ±2 if ΩAr < 20, and require minimal sample preparation, making the technique well suited for testing the sensitivity of coral calcifying fluid ΩAr to ocean acidification and warming using samples from natural and laboratory settings. To demonstrate this, we also show a high-resolution time series of ΩAr over multiple years of growth in a Porites skeleton from the Great Barrier Reef, and we evaluate the response of ΩAr in juvenile Acropora cultured under elevated CO2 and temperature.

  7. Evidence for calcifying nanoparticles in gingival crevicular fluid and dental calculus in periodontitis.

    Science.gov (United States)

    Zhang, Song-Mei; Tian, Fei; Jiang, Xin-Quan; Li, Jing; Xu, Chun; Guo, Xiao-Kui; Zhang, Fu-Qiang

    2009-09-01

    Calcifying nanoparticles (CNPs), also known as nanobacteria, can produce carbonate apatite on their cell walls and initiate pathologic calcification. The objective of this study was to determine whether CNPs are present in the gingival crevicular fluid (GCF) from subjects with periodontal disease and whether they can induce the pathologic calcification of primary cultured human gingival epithelial cells. GCF and dental calculus samples were collected from 10 subjects with gingivitis and 10 subjects with chronic periodontitis. CNPs in GCF and calculus filtrates were detected with nanocapture enzyme-linked immunosorbent assay kits. The CNPs in cultures of dental calculus filtrates were also identified using immunofluorescence staining, transmission electron microscopy (TEM), and chemical analysis. Pathologic changes in the CNP-treated gingival epithelial cells were observed with TEM, alizarin red staining, and disk-scanning confocal microscopy. CNPs were found in GCF samples from two subjects with chronic periodontitis. Based on chemical analysis, the surface-associated material from CNPs isolated and cultured from calculus has a composition similar to dental calculus. The pathologic calcification of CNP-treated gingival epithelial cells was also observed. Self-replicating calcifying nanoparticles can be cultured and identified from dental calculus. This raises the issue of whether CNPs contribute to the pathogenesis of periodontitis.

  8. Pacific-wide contrast highlights resistance of reef calcifiers to ocean acidification.

    Science.gov (United States)

    Comeau, S; Carpenter, R C; Nojiri, Y; Putnam, H M; Sakai, K; Edmunds, P J

    2014-09-07

    Ocean acidification (OA) and its associated decline in calcium carbonate saturation states is one of the major threats that tropical coral reefs face this century. Previous studies of the effect of OA on coral reef calcifiers have described a wide variety of outcomes for studies using comparable partial pressure of CO2 (pCO2) ranges, suggesting that key questions remain unresolved. One unresolved hypothesis posits that heterogeneity in the response of reef calcifiers to high pCO2 is a result of regional-scale variation in the responses to OA. To test this hypothesis, we incubated two coral taxa (Pocillopora damicornis and massive Porites) and two calcified algae (Porolithon onkodes and Halimeda macroloba) under 400, 700 and 1000 μatm pCO2 levels in experiments in Moorea (French Polynesia), Hawaii (USA) and Okinawa (Japan), where environmental conditions differ. Both corals and H. macroloba were insensitive to OA at all three locations, while the effects of OA on P. onkodes were location-specific. In Moorea and Hawaii, calcification of P. onkodes was depressed by high pCO2, but for specimens in Okinawa, there was no effect of OA. Using a study of large geographical scale, we show that resistance to OA of some reef species is a constitutive character expressed across the Pacific. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

  9. Multiple calcifying hyperplastic dental follicles: A case report

    International Nuclear Information System (INIS)

    Aydin, Ulkem; Baykul, Timucin; Yildirim, Benay; Yildirim, Derya; Bozdemir, Esin; Karaduman, Ayse

    2013-01-01

    This report describes a 31-year-old female patient with six impacted teeth. The crowns of the impacted teeth were surrounded with cyst-like lesions with a mixed internal structure and well-defined cortical borders. Microscopic examination of the specimen obtained from the follicle of the left mandibular third molar tooth revealed loose to moderately dense collagenous connective tissue with abundant calcified material and sparse epithelial islands. A diagnosis of multiple calcifying hyperplastic dental follicles was made.

  10. Multiple calcifying hyperplastic dental follicles: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Aydin, Ulkem [Dept. of Dentomaxillofacial Radiology, Baskent University Faculty of Dentistry, Ankara (Turkey); Baykul, Timucin [Dept. of Oral and Maxillofacial Surgery, Suleyman Demirel University Faculty of Dentistry, Isparta (Turkey); Yildirim, Benay [Dept. of Oral Pathology, Gazi University Faculty of Dentistry, Ankara (Turkey); Yildirim, Derya; Bozdemir, Esin [Dept. of Dentomaxillofacial Radiology, Suleyman Demirel University Faculty of Dentistry, Isparta (Turkey); Karaduman, Ayse [Atlas Dent Dental Health Center, Aydin (Turkey)

    2013-12-15

    This report describes a 31-year-old female patient with six impacted teeth. The crowns of the impacted teeth were surrounded with cyst-like lesions with a mixed internal structure and well-defined cortical borders. Microscopic examination of the specimen obtained from the follicle of the left mandibular third molar tooth revealed loose to moderately dense collagenous connective tissue with abundant calcified material and sparse epithelial islands. A diagnosis of multiple calcifying hyperplastic dental follicles was made.

  11. Calcifying tendinitis of the rotator cuff with focal umeral osteolysis. Imaging features

    OpenAIRE

    V. V. Mascarenhas; F. Morais; H. Marques; A. Guerra; E. Carpinteiro; A. Gaspar

    2015-01-01

    Calcifying tendinitis occurs most commonly in the rotator cuff tendons, particularly involving the supraspinatus tendon insertion, and is often asymptomatic. Cortical erosion secondary to calcifying tendinitis has been reported in multiple locations, including in the rotator cuff tendons. The authors report two cases of symptomatic calcifying tendinitis involving the infraspinatus tendon with cortical erosion with correlative radiographic, and MR findings. The importance of considering this d...

  12. Bone stroma-derived cells change coregulators recruitment to androgen receptor and decrease cell proliferation in androgen-sensitive and castration-resistant prostate cancer cells

    International Nuclear Information System (INIS)

    Villagran, Marcelo A.; Gutierrez-Castro, Francisco A.; Pantoja, Diego F.; Alarcon, Jose C.; Fariña, Macarena A.; Amigo, Romina F.; Muñoz-Godoy, Natalia A.; Pinilla, Mabel G.; Peña, Eduardo A.; Gonzalez-Chavarria, Ivan; Toledo, Jorge R.; Rivas, Coralia I.; Vera, Juan C.; McNerney, Eileen M.; Onate, Sergio A.

    2015-01-01

    Prostate cancer (CaP) bone metastasis is an early event that remains inactive until later-stage progression. Reduced levels of circulating androgens, due to andropause or androgen deprivation therapies, alter androgen receptor (AR) coactivator expression. Coactivators shift the balance towards enhanced AR-mediated gene transcription that promotes progression to androgen-resistance. Disruptions in coregulators may represent a molecular switch that reactivates latent bone metastasis. Changes in AR-mediated transcription in androgen-sensitive LNCaP and androgen-resistant C4-2 cells were analyzed for AR coregulator recruitment in co-culture with Saos-2 and THP-1. The Saos-2 cell line derived from human osteosarcoma and THP-1 cell line representing human monocytes were used to display osteoblast and osteoclast activity. Increased AR activity in androgen-resistant C4-2 was due to increased AR expression and SRC1/TIF2 recruitment and decreased SMRT/NCoR expression. AR activity in both cell types was decreased over 90% when co-cultured with Saos-2 or THP-1 due to dissociation of AR from the SRC1/TIF2 and SMRT/NCoR coregulators complex, in a ligand-dependent and cell-type specific manner. In the absence of androgens, Saos-2 decreased while THP-1 increased proliferation of LNCaP cells. In contrast, both Saos-2 and THP-1 decreased proliferation of C4-2 in absence and presence of androgens. Global changes in gene expression from both CaP cell lines identified potential cell cycle and androgen regulated genes as mechanisms for changes in cell proliferation and AR-mediated transactivation in the context of bone marrow stroma cells. - Highlights: • Decreased corepressor expression change AR in androgen-resistance prostate cancer. • Bone stroma-derived cells change AR coregulator recruitment in prostate cancer. • Bone stroma cells change cell proliferation in androgen-resistant cancer cells. • Global gene expression in CaP cells is modified by bone stroma cells in co

  13. Bone stroma-derived cells change coregulators recruitment to androgen receptor and decrease cell proliferation in androgen-sensitive and castration-resistant prostate cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Villagran, Marcelo A.; Gutierrez-Castro, Francisco A.; Pantoja, Diego F.; Alarcon, Jose C.; Fariña, Macarena A.; Amigo, Romina F.; Muñoz-Godoy, Natalia A. [Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion (Chile); Pinilla, Mabel G. [Department of Medical Specialties, School of Medicine, University of Concepcion, Concepcion (Chile); Peña, Eduardo A.; Gonzalez-Chavarria, Ivan; Toledo, Jorge R.; Rivas, Coralia I.; Vera, Juan C. [Department of Physiopathology, School of Biological Sciences, University of Concepcion, Concepcion (Chile); McNerney, Eileen M. [Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion (Chile); Onate, Sergio A., E-mail: sergio.onate@udec.cl [Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion (Chile); Department of Medical Specialties, School of Medicine, University of Concepcion, Concepcion (Chile); Department of Urology, State University of New York at Buffalo, NY (United States)

    2015-11-27

    Prostate cancer (CaP) bone metastasis is an early event that remains inactive until later-stage progression. Reduced levels of circulating androgens, due to andropause or androgen deprivation therapies, alter androgen receptor (AR) coactivator expression. Coactivators shift the balance towards enhanced AR-mediated gene transcription that promotes progression to androgen-resistance. Disruptions in coregulators may represent a molecular switch that reactivates latent bone metastasis. Changes in AR-mediated transcription in androgen-sensitive LNCaP and androgen-resistant C4-2 cells were analyzed for AR coregulator recruitment in co-culture with Saos-2 and THP-1. The Saos-2 cell line derived from human osteosarcoma and THP-1 cell line representing human monocytes were used to display osteoblast and osteoclast activity. Increased AR activity in androgen-resistant C4-2 was due to increased AR expression and SRC1/TIF2 recruitment and decreased SMRT/NCoR expression. AR activity in both cell types was decreased over 90% when co-cultured with Saos-2 or THP-1 due to dissociation of AR from the SRC1/TIF2 and SMRT/NCoR coregulators complex, in a ligand-dependent and cell-type specific manner. In the absence of androgens, Saos-2 decreased while THP-1 increased proliferation of LNCaP cells. In contrast, both Saos-2 and THP-1 decreased proliferation of C4-2 in absence and presence of androgens. Global changes in gene expression from both CaP cell lines identified potential cell cycle and androgen regulated genes as mechanisms for changes in cell proliferation and AR-mediated transactivation in the context of bone marrow stroma cells. - Highlights: • Decreased corepressor expression change AR in androgen-resistance prostate cancer. • Bone stroma-derived cells change AR coregulator recruitment in prostate cancer. • Bone stroma cells change cell proliferation in androgen-resistant cancer cells. • Global gene expression in CaP cells is modified by bone stroma cells in co

  14. Calcifying invertebrates succeed in a naturally CO2-rich coastal habitat but are threatened by high levels of future acidification

    Directory of Open Access Journals (Sweden)

    M. Wahl

    2010-11-01

    Full Text Available CO2 emissions are leading to an acidification of the oceans. Predicting marine community vulnerability towards acidification is difficult, as adaptation processes cannot be accounted for in most experimental studies. Naturally CO2 enriched sites thus can serve as valuable proxies for future changes in community structure. Here we describe a natural analogue site in the Western Baltic Sea. Seawater pCO2 in Kiel Fjord is elevated for large parts of the year due to upwelling of CO2 rich waters. Peak pCO2 values of >230 Pa (>2300 μatm and pHNBS values of pCO2 values are ~70 Pa (~700 μatm. In contrast to previously described naturally CO2 enriched sites that have suggested a progressive displacement of calcifying auto- and heterotrophic species, the macrobenthic community in Kiel Fjord is dominated by calcifying invertebrates. We show that blue mussels from Kiel Fjord can maintain control rates of somatic and shell growth at a pCO2 of 142 Pa (1400 μatm, pHNBS = 7.7. Juvenile mussel recruitment peaks during the summer months, when high water pCO2 values of ~100 Pa (~1000 μatm prevail. Our findings indicate that calcifying keystone species may be able to cope with surface ocean pHNBS values projected for the end of this century when food supply is sufficient. However, owing to non-linear synergistic effects of future acidification and upwelling of corrosive water, peak seawater pCO2 in Kiel Fjord and many other productive estuarine habitats could increase to values >400 Pa (>4000 μatm. These changes will most likely affect calcification and recruitment, and increase external shell dissolution.

  15. Calcifying mucinous adenocarcinoma of the stomach: Report of two cases

    International Nuclear Information System (INIS)

    Sung, Ki Yeal; Han, Seong Tai; Bahk, Yong Whee

    1983-01-01

    There is a great variety of abdominal calcifications of varying etiology. Among them, calcification in gastric carcinoma is rare. To the best of our knowledge, there has been no previous report of calcifying mucinous adenocarcinoma of the stomach in Korea. In the present communication, we report toe cases of this rare tumor. The first cases was 27 years old female who had anorexia and palpable mass in the epigastrium. By palpation, a firm non-tender large mass was felt in the epigastrium. A plain abdominal film showed numerous punctuate calcifications in the left upper quadrant. Film from an upper G-I series demonstrated findings of advanced gastric carcinoma with multiple punctuate calcifications involving the antrum and body. Gastroscopic biopsy proved the lesion to be mucinous adenocarcinoma (signet ring cell type). The second case was 38 years old female who complicated of nausea, vomiting and weight loss. On physical examination, she appeared normal. Routine laboratory tests were within normal limits. A plain abdominal film reveal stippled calcifications in the left upper quadrant medial to the splenic shadow. The film from an upper G-I series showed a mass in the fundus and upper body of stomach with multiple stippled calcifications along the lesser curvature. Subtotal gastrectomy was performed and the pathologic finding was calcifying mucinous adenocarcinoma (signet ring cell type)

  16. Successful penetration and bougie dilatation with Brockenbrough needle for severely calcified occlusion in superficial femoral artery.

    Science.gov (United States)

    Makita, Toshio; Suzuki, Kenji; Takizawa, Kaname; Ootomo, Tatsushi; Inoue, Naoto; Meguro, Taiichirou

    2014-04-01

    A 75-year-old hemodialysis patient with right critical limb ischemia received endovascular therapy for a chronic total occlusion (CTO) in a diffusely calcified superficial femoral artery (SFA). During a retrograde approach, a Brockenbrough needle (BN) was able to penetrate the calcified hard plaque formed in the middle segment of the CTO. Moreover, bougie dilatation with the BN allowed balloon crossing and stent deployment, even after failure to pass a 2.0-mm monorail balloon across the plaque. These results suggest that the BN offers a new therapeutic option in the penetration and modification of severely calcified CTO in SFA.

  17. Gastric Calcifying Fibrous Tumour

    Directory of Open Access Journals (Sweden)

    Tan Attila

    2006-01-01

    Full Text Available Intramucosal gastric tumours are most commonly found to be gastrointestinal stromal tumours or leiomyomas (smooth muscle tumours; however, a variety of other uncommon mesenchymal tumours can occur in the stomach wall. A rare benign calcifying fibrous tumour is reported and the endoscopic appearance, ultrasound findings and morphology are documented. A review of the literature found only two similar cases.

  18. Sao Luiz do Paraltinga underwater

    International Nuclear Information System (INIS)

    Auada, F.; Angueira, A.; Bakiewicz, G.; Cerqueira, D.; Maganini, E.; Morais, C.

    2012-01-01

    Sao Luiz do Paaitinga, a city of Sao Paulo, Brazil, is considered a cultural and historical heritage of the country. On January first, of 2010 the city was hit by a devasting flood which resulted in the loss and destruction of historical and public buildings, monuments and houses. The referred places suffered damage in their structure and loss of physical and electronic documents. Most of the population lost its legal identity. The nucleo de Conservacao do Arquivo Publico do Estado de Sao Paulo - Nucleus for Conservation of public files of Sao Paulo- APESP and the Nucleo de Conservacao e Restauro Edson Motta, laboratory from National Service for Industrial Apprenticeship, became associated in order to save the documents. the access to the documents was only autorized 26 days after the flooding due to bureaucratic reasons. With few economic ressources and no time to come up with a big plan but only necessary practical courses of action, a completely manual method was established to recover this documents, with the exception of the third allotment. It had to be submitted to gamma-rays cobalt-60 from a multi purpose compact type irradiator from the Centro de Tecnologia das radiacoes do Instituto de Pesquisas Energeticas e Nucleares due to enormous fungus infection. Describing the methodology applied to recover all documents and comparing the third allotment with the others which did not receive irradiation, are the purpose of this paper.

  19. A voltage-gated H+ channel underlying pH homeostasis in calcifying coccolithophores.

    Directory of Open Access Journals (Sweden)

    Alison R Taylor

    2011-06-01

    Full Text Available Marine coccolithophorid phytoplankton are major producers of biogenic calcite, playing a significant role in the global carbon cycle. Predicting the impacts of ocean acidification on coccolithophore calcification has received much recent attention and requires improved knowledge of cellular calcification mechanisms. Uniquely amongst calcifying organisms, coccolithophores produce calcified scales (coccoliths in an intracellular compartment and secrete them to the cell surface, requiring large transcellular ionic fluxes to support calcification. In particular, intracellular calcite precipitation using HCO₃⁻ as the substrate generates equimolar quantities of H+ that must be rapidly removed to prevent cytoplasmic acidification. We have used electrophysiological approaches to identify a plasma membrane voltage-gated H+ conductance in Coccolithus pelagicus ssp braarudii with remarkably similar biophysical and functional properties to those found in metazoans. We show that both C. pelagicus and Emiliania huxleyi possess homologues of metazoan H(v1 H+ channels, which function as voltage-gated H+ channels when expressed in heterologous systems. Homologues of the coccolithophore H+ channels were also identified in a diversity of eukaryotes, suggesting a wide range of cellular roles for the H(v1 class of proteins. Using single cell imaging, we demonstrate that the coccolithophore H+ conductance mediates rapid H+ efflux and plays an important role in pH homeostasis in calcifying cells. The results demonstrate a novel cellular role for voltage gated H+ channels and provide mechanistic insight into biomineralisation by establishing a direct link between pH homeostasis and calcification. As the coccolithophore H+ conductance is dependent on the trans-membrane H+ electrochemical gradient, this mechanism will be directly impacted by, and may underlie adaptation to, ocean acidification. The presence of this H+ efflux pathway suggests that there is no obligate

  20. A calcified epidermoid cyst within right lateral ventricle: A report of a rare case

    Directory of Open Access Journals (Sweden)

    Ranjan Kumar Sahoo

    2016-01-01

    Full Text Available A young patient reported to neurosurgery outdoor department with symptoms of increased intracranial pressure. Noncontrast computed tomography examination showed a single calcified mass within right lateral ventricle with mild hydrocephalus. Contrast-enhanced magnetic resonance imaging revealed nonenhancing single mass within right lateral ventricle with mild hydrocephalus. Intraventricular calcified choroid papilloma/calcified epidermoid were radiological differentials. The mass was excised, removed from the lateral ventricles and found to be calcified epidermoid on gross and microscopic examination, which is rare.

  1. How Should We Perform Rotational Atherectomy to an Angulated Calcified Lesion?

    Science.gov (United States)

    Sakakura, Kenichi; Taniguchi, Yousuke; Matsumoto, Mitsunari; Wada, Hiroshi; Momomura, Shin-Ichi; Fujita, Hideo

    2016-05-25

    Rotational atherectomy to an angulated calcified lesion is always challenging. The risk of catastrophic complications such as a burr becoming stuck or vessel perforation is greater when the calcified lesion is angulated. We describe the case of an 83-year-old female suffering from unstable angina. Diagnostic coronary angiography revealed an angulated calcified lesion in the proximal segment of the right coronary artery. We performed rotational atherectomy to the lesion, but intentionally did not advance the rotational atherectomy burr beyond the top of the angulation. We controlled the rotational atherectomy burr and stopped it just before the top of the angulation to avoid complications. Following rotational atherectomy, balloon dilatation with a non-compliant balloon was performed, and drug-eluting stents were successfully deployed. In this manuscript, we provide a review of the literature on this topic, and discuss how rotational atherectomy to an angulated calcified lesion should be performed.

  2. Coral calcifying fluid pH is modulated by seawater carbonate chemistry not solely seawater pH.

    Science.gov (United States)

    Comeau, S; Tambutté, E; Carpenter, R C; Edmunds, P J; Evensen, N R; Allemand, D; Ferrier-Pagès, C; Tambutté, S; Venn, A A

    2017-01-25

    Reef coral calcification depends on regulation of pH in the internal calcifying fluid (CF) in which the coral skeleton forms. However, little is known about calcifying fluid pH (pH CF ) regulation, despite its importance in determining the response of corals to ocean acidification. Here, we investigate pH CF in the coral Stylophora pistillata in seawater maintained at constant pH with manipulated carbonate chemistry to alter dissolved inorganic carbon (DIC) concentration, and therefore total alkalinity (A T ). We also investigate the intracellular pH of calcifying cells, photosynthesis, respiration and calcification rates under the same conditions. Our results show that despite constant pH in the surrounding seawater, pH CF is sensitive to shifts in carbonate chemistry associated with changes in [DIC] and [A T ], revealing that seawater pH is not the sole driver of pH CF Notably, when we synthesize our results with published data, we identify linear relationships of pH CF with the seawater [DIC]/[H + ] ratio, [A T ]/ [H + ] ratio and [[Formula: see text

  3. Environmental radiation monitoring in Sao Paulo state

    International Nuclear Information System (INIS)

    Agudo, E.G.; Albuquerque, A.M. de; Vasconcellos, N.V. de

    1991-01-01

    The results of environmental radiation monitoring that CETESB (Technological Company of Environmental Sanitation of Sao Paulo) does in influence areas of radioactive materials store of Nuclemon Minero-Quimica S.A. in the municipality of Itu, Sao Paulo State, are presented. The data comprises the period of 1983-1990 with information about concentration levels of radium-225 in underground and superficial waters of the region. On March, 1989, was detected contamination in a well near of the stores. The levels observed, its implications in terms of health risks and possible causes of event are discussed too. The goals that CETESB intends to reach in terms of monitoring of environmental radiation in Sao Paulo State are also presented. (C.M.)

  4. Orbital atherectomy for treating de novo, severely calcified coronary lesions: 3-year results of the pivotal ORBIT II trial.

    Science.gov (United States)

    Lee, Michael; Généreux, Philippe; Shlofmitz, Richard; Phillipson, Daniel; Anose, Bynthia M; Martinsen, Brad J; Himmelstein, Stevan I; Chambers, Jeff W

    2017-06-01

    The presence of heavy coronary artery calcification increases the complexity of percutaneous coronary intervention (PCI) and increases the incidence of major adverse cardiac events (MACE): death, myocardial infarction (MI), target vessel revascularization (TVR), and stent thrombosis. The ORBIT II (Evaluate the Safety and Efficacy of OAS in Treating Severely Calcified Coronary Lesions) trial reported low rates of procedural, 30-day, 1-year, and 2-year ischemic complications after treatment of de novo, severely calcified lesions with the Diamondback 360° Coronary Orbital Atherectomy System (OAS) (Cardiovascular Systems, Inc.). ORBIT II was a single-arm trial that enrolled 443 patients at 49U.S. sites; in this study, de novo, severely calcified coronary lesions were treated with OAS prior to stenting. The primary safety endpoint was 30-day MACE: the composite of cardiac death, MI, and TVR (inclusive of target lesion revascularization (TLR)). The primary efficacy endpoint was procedural success: stent delivery with a residual stenosis of atherectomy. There were 360 (81.3%) subjects who completed the protocol-mandated 3-year visit.The overall cumulative rate of 3-year MACE was 23.5%, including cardiac death (6.7%), MI (11.2%), and TVR (10.2%). The 3-year target lesion revascularization rate was 7.8%. In the final 3-year analysis of the ORBIT II trial, orbital atherectomy of severely calcified coronary lesions followed by stenting resulted in a low rate of adverse ischemic events compared with historical controls.Orbital atherectomy represents a safe and effective revascularization strategy for patients with severely calcified coronary lesions. The ORBIT II trial enrolled 443 subjects to study orbital atherectomy followed by stenting for de novo severely calcified coronary lesions. The overall cumulative 3-year MACE rate was 23.5%, including cardiac death (6.7%), MI (11.2%), and TVR (10.2%); the 3-year target lesion revascularization rate was 7.8%. Orbital atherectomy

  5. Energy balance of the Sao Paulo State - 1990

    International Nuclear Information System (INIS)

    1992-01-01

    This document informs the energetic balance for Sao Paulo State - 1990, with information referring to the year 1989, containing the energy fluxes from primary and secondary energy sources in the main sectors of Sao Paulo economy. 32 figs., 99 tabs

  6. Case report: Calcified chronic subdural haematoma in an elderly ...

    African Journals Online (AJOL)

    Calcified chronic subdural haematoma [CCSDH] is a rare complication of the relatively more common condition of Chronic Subdural Haematoma (CSDH). We present the case of a 68yr old man referred with a 2 week history of sudden onset Right hemi paresis generalized tonic clonic seizures and aphasia. There was a ...

  7. Different surface sensing of the cell body and nucleus in healthy primary cells and in a cancerous cell line on nanogrooves.

    Science.gov (United States)

    Davidson, Patricia M; Bigerelle, Maxence; Reiter, Günter; Anselme, Karine

    2015-10-01

    Cancer cells are known to have alterations compared to healthy cells, but can these differences extend to the way cells interact with their environment? Here, the authors focused on the alignment on an array of grooves of nanometer depth using two cell types: healthy osteoprogenitor primary cells (HOP) and a cancerous osteosarcoma (SaOs-2) cell line. Another concern was how this alignment affects the cell's interior, namely, the nucleus. Based on the results, it is proposed that these two cell types respond to different size regimes: SaOs-2 cells are more sensitive to shallow grooves while HOP cells are strongly aligned with deep grooves. As a measure of the impact of cell alignment on the nucleus the orientation and elongation of the nucleus were determined. Compared to HOP cells, the cell nucleus of SaOs-2 cells is more aligned and elongated in response to grooves, suggesting a softer nucleus and/or increased force transmission. These results support the hypothesis that cancer cells have reduced nucleus rigidity compared to healthy ones and further indicate differences in sensing, which may be important during metastasis.

  8. Sanguinarine induces apoptosis of human osteosarcoma cells through the extrinsic and intrinsic pathways

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hyunjin; Bergeron, Eric; Senta, Helena; Guillemette, Kim [Cell-Biomaterial Biohybrid Systems, Universite de Sherbrooke, Department of Chemical Engineering and Biotechnological Engineering, Canada J1K 2R1 (Canada); Beauvais, Sabrina [Cell-Biomaterial Biohybrid Systems, Universite de Sherbrooke, Department of Chemical Engineering and Biotechnological Engineering, Canada J1K 2R1 (Canada); Development of Bioprocess, Universite de Sherbrooke, Department of Chemical Engineering and Biotechnological Engineering, Canada J1K 2R1 (Canada); Blouin, Richard [Universite de Sherbrooke, Department of Biology, Canada J1K 2R1 (Canada); Sirois, Joel [Development of Bioprocess, Universite de Sherbrooke, Department of Chemical Engineering and Biotechnological Engineering, Canada J1K 2R1 (Canada); Faucheux, Nathalie, E-mail: Nathalie.Faucheux@Usherbrooke.ca [Cell-Biomaterial Biohybrid Systems, Universite de Sherbrooke, Department of Chemical Engineering and Biotechnological Engineering, Canada J1K 2R1 (Canada)

    2010-08-27

    Research highlights: {yields} We show for the first time the effect of sanguinarine (SA) on MG63 and SaOS-2 cells. {yields} SA altered osteosarcoma cell viability in a concentration and time dependent manner. {yields} SA induced osteosarcoma cell apoptosis and increased caspase-8 and -9 activities. {yields} SA decreased dose dependently the Bcl-2 protein level only in MG63 cells. {yields} SaOS-2 which are osteoblast-derived, seemed more resistant to SA than MG63. -- Abstract: The quaternary benzo[c]phenanthridine alkaloid sanguinarine inhibits the proliferation of cancerous cells from different origins, including lung, breast, pancreatic and colon, but nothing is known of its effects on osteosarcoma, a primary malignant bone tumour. We have found that sanguinarine alters the morphology and reduces the viability of MG-63 and SaOS-2 human osteosarcoma cell lines in concentration- and time-dependent manner. Incubation with 1 {mu}mol/L sanguinarine for 4 and 24 h killed more efficiently MG-63 cells than SaOS-2 cells, while incubation with 5 {mu}mol/L sanguinarine killed almost 100% of both cell populations within 24 h. This treatment also changed the mitochondrial membrane potential in both MG-63 and SaOS-2 cells within 1 h, caused chromatin condensation and the formation of apoptotic bodies. It activated multicaspases, and increased the activities of caspase-8 and caspase-9 in both MG-63 and SaOS-2 cells. These data highlight sanguinarine as a novel potential agent for bone cancer therapy.

  9. Severe calcified stylohyoid complex in twins: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jo Eun; Min, Jung Hyun; Park, Hae Rang; Choi, Bo Ram; Huh, Kyung Hoe [School of Dentistry, Seoul National University, Seoul (Korea, Republic of); Choi, Jin Woo [Dankook University College of Dentistry, Cheonan (Korea, Republic of)

    2012-06-15

    The styloid process is a cylindrical, long cartilaginous bone located on the temporal bone. The calcified stylohyoid ligament and elongated styloid process can be identified radiographically, and they are associated with a number of syndromes and symptoms. The exact cause of the styloid process elongation due to calcification and subsequent ossification of ligament is unclear. This report presents a case of severely calcified stylohyoid ligament complex occurred in twins who have the same pattern of calcification.

  10. Calcifying Odontogenic Cyst Associated with an Impacted Upper Cuspid

    International Nuclear Information System (INIS)

    Yoon, Suk Ja; Lee, Young Seo; Yi, Jae Seo

    2000-01-01

    A 35-year-old man was referred to the department of Oral and maxillofacial surgery of Chonnam university hospital for the chief complaint of asymptomatic swelling on the buccal vestibule of upper right canine area. Radiographs revealed that the upper right canine was impacted and there was a well-circumscribed pericoronal radiolucency related with the canine. Multiple radiopaque foci were scattered in the radiolucent lesion, and the roots of the lateral incisor and the first premolar related to the lesion showed external resorption. The radiographic features of this lesion were typical of adenomatoid odontogenic tumor, but considering the sex and age of the patient, the tentative diagnosis was made as calcifying odontogenic cyst. Microscopically this lesion was diagnosed as calcifying odontogenic cyst. Because calcifying odontogenic cyst has no pathognomonic feature of radiographs, to consider radiographic features with clinical findings is necessary in order to establish more correct diagnosis.

  11. Sao Paulo jätkab biennaali / Maria-Kristiina Soomre

    Index Scriptorium Estoniae

    Soomre, Maria-Kristiina, 1978-

    2001-01-01

    Sao Paulo biennaal sai 50aastaseks. Kuni 29. VII toimunud juubelinäitusest. 2002. a. leiab aset XXV Sao Paulo biennaal "Metropoliikonograafiad", kus esinevad kunstnikud kaheksast maailma suurlinnast.

  12. Sao Paulo pelletron accelerator: fortieth anniversary

    International Nuclear Information System (INIS)

    Pereira, Dirceu

    2012-01-01

    Full text: This year the 8MV Sao Paulo Pelletron tandem accelerator completes 40 years . This electrostatic accelerator was installed in the Sao Paulo University in 1972 , and it was the first of this model constructed the National Electrostatic Corporation with several innovations particularly with respect to the new concept of accelerator tube and the charge system. In the talk will be discussed the performance of the accelerator during all these years and the main result scientific results. (author)

  13. Sao Paulo pelletron accelerator: fortieth anniversary

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Dirceu [Universidade de Sao Paulo (IF/USP), SP (Brazil). Inst. de Fisica

    2012-07-01

    Full text: This year the 8MV Sao Paulo Pelletron tandem accelerator completes 40 years . This electrostatic accelerator was installed in the Sao Paulo University in 1972 , and it was the first of this model constructed the National Electrostatic Corporation with several innovations particularly with respect to the new concept of accelerator tube and the charge system. In the talk will be discussed the performance of the accelerator during all these years and the main result scientific results. (author)

  14. A calcified epidermoid cyst within right lateral ventricle: A report of a rare case

    OpenAIRE

    Ranjan Kumar Sahoo; Debahuti Mohapatra; Pradipta Tripathy

    2016-01-01

    A young patient reported to neurosurgery outdoor department with symptoms of increased intracranial pressure. Noncontrast computed tomography examination showed a single calcified mass within right lateral ventricle with mild hydrocephalus. Contrast-enhanced magnetic resonance imaging revealed nonenhancing single mass within right lateral ventricle with mild hydrocephalus. Intraventricular calcified choroid papilloma/calcified epidermoid were radiological differentials. The mass was excised, ...

  15. Genetic basis of calcifying cystic odontogenic tumors.

    Directory of Open Access Journals (Sweden)

    Akane Yukimori

    Full Text Available Calcifying cystic odontogenic tumors (CCOTs are benign cystic tumors that form abnormally keratinized ghost cells. Mutations in CTNNB1, which encodes beta-catenin, have been implicated in the development of these tumors, but a causal relationship has not been definitively established. Thus, mutational hot spots in 50 cancer genes were examined by targeted next-generation sequencing in 11 samples of CCOT. Mutations in CTNNB1, but not in other genes, were observed in 10 of 11 cases. These mutations constitutively activate beta-catenin signaling by abolishing the phosphorylation sites Asp32, Ser33, or Ser37, and are similar to those reported in pilomatrixoma and adamantinomatous craniopharyngioma. In contrast, BRAF or NRAS mutations were observed in 12 and two control samples of ameloblastoma, respectively. In HEK293 cells, overexpression of mutated CTNNB1 also upregulated hair keratin, a marker of ghost cells. Furthermore, ghost cells were present in two cases of ameloblastoma with BRAF and CTNNB1 mutations, indicating that ghost cells form due to mutations in CTNNB1. The data suggest that mutations in CTNNB1 are the major driver mutations of CCOT, and that CCOT is the genetic analog of pilomatrixoma and adamantinomatous craniopharyngioma in odontogenic tissue.

  16. Calcified Plaque of Coronary Artery: Factors Influencing Overestimation of Coronary Artery Stenosis on Coronary CT Angiography

    International Nuclear Information System (INIS)

    Kim, Mok Hee; Kim, Yun Hyeon; Choi, Song; Seon, Hyun Ju; Jeong, Gwang Woo; Park, Jin Gyoon; Kang, Heoung Keun; Ko, Joon Seok

    2010-01-01

    To assess the influence of calcified plaque characteristics on the overestimation of coronary arterial stenosis on a coronary CT angiography (CCTA). The study included 271 coronary arteries with calcified plaques identified by CCTA, and based on 928 coronary arteries from 232 patients who underwent both CCTA and invasive coronary angiography (ICA). Individual coronary arteries were classified into two groups by agreement based on the degree of stenosis from each CCTA and ICA: 1) group A includes patients with concordant CCTA and ICA results and, 2) group B includes patients with an overestimation of CCTA compared to ICA. Parameters including total calcium score, calcium score of an individual coronary artery, calcium burden number of an individual coronary artery, and the density of each calcified plaque (calcium score / number of calcium burden) for each individual coronary artery were compared between the two groups. Of the 271 coronary arteries, 164 (60.5%) were overestimated on CCTA. The left anterior descending artery (LAD) had a significantly low rate of overestimation (47.1%) compared to the other coronary arteries (p=0.001). No significant differences for total calcium score, calcium score of individual coronary artery, and the density of each calcified plaque from individual coronary arteries between two groups was observed. However, a decreasing tendency for the rate of overestimation on CCTA was observed with an increase in calcium burden of individual coronary arteries (p<0.05). The evaluation of coronary arteries suggests that the degree of coronary arterial stenosis had a tendency to be overestimated by calcified plaques on CCTA. However, the rate of overestimation for the degree of coronary arterial stenosis by calcified plaques was not significantly influenced by total calcium score, calcium score of individual coronary artery, and density of each calcified plaque

  17. Lumen and calcium characteristics within calcified coronary lesions. Comparison of computed tomography coronary angiography versus intravascular ultrasound.

    Science.gov (United States)

    Noll, Dariusz; Kruk, Mariusz; Pręgowski, Jerzy; Kaczmarska, Edyta; Kryczka, Karolina; Pracoń, Radosław; Skwarek, Mirosław; Dzielińska, Zofia; Petryka, Joanna; Spiewak, Mateusz; Lubiszewska, Barbara; Norwa-Otto, Bożena; Opolski, Maksymilian; Witkowski, Adam; Demkow, Marcin; Rużyłło, Witold; Kępka, Cezary

    2013-01-01

    Computed tomography coronary angiography (CTCA) is a diagnostic method used for exclusion of coronary artery disease. However, lower accuracy of CTCA in assessment of calcified lesions is a significant factor impeding applicability of CTCA for assessment of coronary atherosclerosis. To provide insight into lumen and calcium characteristics assessed with CTCA, we compared these parameters to the reference of intravascular ultrasound (IVUS). Two hundred and fifty-two calcified lesions within 97 arteries of 60 patients (19 women, age 63 ±10 years) underwent assessment with both 2 × 64 slice CT (Somatom Definition, Siemens) and IVUS (s5, Volcano Corp.). Coronary lumen and calcium dimensions within calcified lesions were assessed with CTCA and compared to the reference measurements made with IVUS. On average CTCA underestimated mean lumen diameter (2.8 ±0.7 mm vs. 2.9 ±0.8 mm for IVUS), lumen area (6.4 ±3.4 mm(2) vs. 7.0 ±3.7 mm(2) for IVUS, p < 0.001) and total calcium arc (52 ±35° vs. 83 ±54°). However, analysis of tertiles of the examined parameters revealed that the mean lumen diameter, lumen area and calcium arc did not significantly differ between CTCA and IVUS within the smallest lumens (1(st) tertile of mean lumen diameter at 2.1 mm, and 1(st) tertile of lumen area at 3.7 mm(2)) and lowest calcium arc (mean of 40°). Although, on average, CTCA underestimates lumen diameter and area as well as calcium arc within calcified lesions, the differences are not significant within the smallest vessels and calcium arcs. The low diagnostic accuracy of CTCA within calcified lesions may be attributed to high variance and not to systematic error of measurements.

  18. Energy balance of the Sao Paulo State - 1995

    International Nuclear Information System (INIS)

    1996-01-01

    This work informs the energetic balance of Sao Paulo State - 1995, with information referring to the year 1994, containing the energy fluxes from primary and secondary energy sources in the main sectors of Sao Paulo economy. An electronic version for windows environment is also available. 81 figs., 179 tabs

  19. Small calcified lesions suggestive of neurocysticercosis are associated with mesial temporal sclerosis

    Directory of Open Access Journals (Sweden)

    Marcos C. B. Oliveira

    2014-07-01

    Full Text Available Recent studies have suggested a possible relationship between temporal lobe epilepsy with mesial temporal sclerosis (MTS and neurocysticercosis (NC. We performed a case-control study to evaluate the association of NC and MTS. Method: We randomly selected patients with different epilepsy types, including: MTS, primary generalized epilepsy (PGE and focal symptomatic epilepsy (FSE. Patients underwent a structured interview, followed by head computed tomography (CT. A neuroradiologist evaluated the scan for presence of calcified lesions suggestive of NC. CT results were matched with patients’ data. Results: More patients in the MTS group displayed calcified lesions suggestive of NC than patients in the other groups (p=0.002. On multivariate analysis, MTS was found to be an independent predictor of one or more calcified NC lesions (p=0.033. Conclusion: After controlling for confounding factors, we found an independent association between NC calcified lesions and MTS.

  20. Outcomes After Orbital Atherectomy of Severely Calcified Left Main Lesions: Analysis of the ORBIT II Study.

    Science.gov (United States)

    Lee, Michael S; Shlofmitz, Evan; Shlofmitz, Richard; Sahni, Sheila; Martinsen, Brad; Chambers, Jeffrey

    2016-09-01

    The ORBIT II trial reported excellent outcomes in patients with severely calcified coronary lesions treated with orbital atherectomy. Severe calcification of the left main (LM) artery represents a complex coronary lesion subset. This study evaluated the safety and efficacy of coronary orbital atherectomy to prepare severely calcified protected LM artery lesions for stent placement. The ORBIT II trial was a prospective, multicenter clinical trial that enrolled 443 patients with severely calcified coronary lesions in the United States. The major adverse cardiac event (MACE) rate through 2 years post procedure, defined by cardiac death, myocardial infarction (CK-MB >3x upper limit of normal with or without a new pathologic Q-wave) and target-vessel revascularization, was compared in the LM and non-left main (NLM) groups. Among the 443 patients, a total of 10 underwent orbital atherectomy of protected LM artery lesions. At 2 years, there was no significant difference in the 2-year MACE rate in the LM and NLM groups (30.0% vs 19.1%, respectively; P=.36). Cardiac death was low in both groups (0% vs 4.4%, respectively; P=.99). Myocardial infarction occurred within 30 days in both groups (10.0% vs 9.7%, respectively; P=.99). Severe dissection, perforation, persistent slow flow, and persistent no reflow did not occur in the LM group. Abrupt closure occurred in 1 patient in the LM group. Orbital atherectomy for patients with heavily calcified LM coronary artery lesions is safe and feasible. Further studies are needed to assess the safety and efficacy of orbital atherectomy in patients with severely calcified LM artery lesions.

  1. Comparison of the reaction of bone-derived cells to enhanced MgCl2-salt concentrations.

    Science.gov (United States)

    Burmester, Anna; Luthringer, Bérengère; Willumeit, Regine; Feyerabend, Frank

    2014-01-01

    Magnesium-based implants exhibit various advantages such as biodegradability and potential for enhanced in vivo bone formation. However, the cellular mechanisms behind this possible osteoconductivity remain unclear. To determine whether high local magnesium concentrations can be osteoconductive and exclude other environmental factors that occur during the degradation of magnesium implants, magnesium salt (MgCl2) was used as a model system. Because cell lines are preferred targets in studies of non-degradable implant materials, we performed a comparative study of 3 osteosarcoma-derived cell lines (MG63, SaoS2 and U2OS) with primary human osteoblasts. The correlation among cell count, viability, cell size and several MgCl2 concentrations was used to examine the influence of magnesium on proliferation in vitro. Moreover, bone metabolism alterations during proliferation were investigated by analyzing the expression of genes involved in osteogenesis. It was observed that for all cell types, the cell count decreases at concentrations above 10 mM MgCl2. However, detailed analysis showed that MgCl2 has a relevant but very diverse influence on proliferation and bone metabolism, depending on the cell type. Only for primary cells was a clear stimulating effect observed. Therefore, reliable results demonstrating the osteoconductivity of magnesium implants can only be achieved with primary osteoblasts.

  2. Preoperative computed tomography of the chest in lung cancer patients: the predictive value of calcified lymph nodes for the perioperative outcomes of video-assisted thoracoscopic surgery lobectomy

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Kwang Nam; Lee, Youkyung; Wi, Jae Yeon [Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Department of Radiology, Seoul (Korea, Republic of); Moon, Hyeon-Jong; Sung, Yong Won [Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Department of Cardiothoracic Surgery, Seoul (Korea, Republic of)

    2013-12-15

    To determine the predictive value of identifying calcified lymph nodes (LNs) for the perioperative outcomes of video-assisted thoracoscopic surgery (VATS). Fifty-six consecutive patients who underwent VATS lobectomy for lung cancer were included. We evaluated the number and location of calcified LNs on computed tomography (CT). We investigated clinical parameters, including percentage forced expiratory volume in 1 s (FEV{sub 1}%), surgery duration, chest tube indwelling duration, and length of hospital stay. We performed linear regression analysis and multiple comparisons of perioperative outcomes. Mean number of calcified LNs per patient was 0.9 (range, 0-6), mostly located in the hilar-interlobar zone (43.8 %). For surgery duration (mean, 5.0 h), FEV{sub 1}% and emphysema severity were independent predictors (P = 0.010 and 0.003, respectively). The number of calcified LNs was an independent predictor for chest tube indwelling duration (P = 0.030) and length of hospital stay (P = 0.046). Mean duration of chest tube indwelling and hospital stay was 8.8 days and 12.7 days in no calcified LN group; 9.2 and 13.2 in 1 calcified LN group; 12.8 and 19.7 in {>=}2 calcified LNs group, respectively. The presence of calcified LNs on CT can help predict more complicated perioperative course following VATS lobectomy. (orig.)

  3. Metal and trace element sediment assessment from two estuarine systems: Santos/Sao Vicente and Cananeia, State of Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Amorim, Eduardo Paulo de

    2012-01-01

    This study evaluated some toxic metals such as Cd, Hg and Pb and some other major and trace elements in surface sediment samples, from two different systems under different degrees of anthropogenic actions: the estuarine system of Santos/Sao Vicente and the southern part of the Cananeia estuary, both on the Sao Paulo state coast. Sediment samples were collected in 16 stations in the Santos/Sao Vicente estuary and 13 stations in the Cananeia estuary, during summer and winter of 2005 and 2006, in both estuaries. Three analytical techniques were used: NAA, AAS and ICP OES. NAA was used for the quantification of major element concentration levels (Ca, Fe and Na), trace elements (As, Ba, Br, Cd, Co, Cu, Cr, Cs, Hf, Rb, Sb, Sc, Se, Ta, Th, U, and Zn and rare earths elements La, Ce, Eu, Nd, Sm, Lu, Tb, Yb). ICP OES was used for determination of the concentration levels of Al, Ba, Be, Bi, B, Cd, Co, Pb, Cu, Cr, Sn, Sr, Fe, Li, Mn, Mo, Ni, Tl, Ti, V and Zn. AAS for Cd and Pb quantification through graphite furnace (GF AAS) and Hg through cold vapor generation (CV AAS). Methodology validation according to precision and accuracy was performed by reference material analyses for the three analytical techniques used. Detection and quantification limits were calculated for each element evaluated. Seasonal variations (summer and winter), spatial and temporal (2005 e 2006) variations of metals and trace elements were also evaluated. In the Santos estuary, in general, metal and trace element concentrations , organic matter content and % of pelitic fraction found in the Santos channel (area 1) were higher than those of the Santos Bay (area 2) and Sao Vicente channel (area 3). Area 1 suffers high impact from industrial activities from the Cubatao region and Santos port. The sediments from station 14 (area 3, Sao Vicente channel), showed the same behavior of those from area 1, suffering influence from the industrial pole and located in a mangrove area. In comparison with TEL and PEL

  4. Ameloglastic fibro-odontoma with a change of calcifying odontogenic cyst

    International Nuclear Information System (INIS)

    Kwon, Hyuk Rok; Han, Jin Woo; Lee, Jin Ho; Choi, Hang Moon; Park, In Woo; Lee, Suk Keun

    2001-01-01

    Thirteen-year-old girl complaining of the swelling and pain on the left midface visited our dental hospital. On the radiographic examination, well-defined radiolucent lesion with hyperostotic border was found in the left maxilla accompanying with the external root resorption of the involved teeth and the displaced second molar. CT showed calcified bodies, thinning of hard palate, inferior orbital wall and lateral wall of nasal fossa, and thinning and perforation of the buccal plate of the maxilla. Enucleation and curettage of the lesion and nasoantrostomy was carried out and histopathologic examination mainly showed a solid tumor tissue composed of odontogenic epithelium and pulp tissues admixed with dentin and enamel formation. And some part of reduced follicular epithelium of tooth germ showed a change mimicking calcifying odontogenic cyst. Taken together, we concluded the lesion is an ameloblastic fibro-odontoma with as change of calcifying odontogenic cyst

  5. Ameloglastic fibro-odontoma with a change of calcifying odontogenic cyst

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, Hyuk Rok; Han, Jin Woo; Lee, Jin Ho; Choi, Hang Moon; Park, In Woo; Lee, Suk Keun [Kangnung National Univ., Kangnung (Korea, Republic of)

    2001-09-15

    Thirteen-year-old girl complaining of the swelling and pain on the left midface visited our dental hospital. On the radiographic examination, well-defined radiolucent lesion with hyperostotic border was found in the left maxilla accompanying with the external root resorption of the involved teeth and the displaced second molar. CT showed calcified bodies, thinning of hard palate, inferior orbital wall and lateral wall of nasal fossa, and thinning and perforation of the buccal plate of the maxilla. Enucleation and curettage of the lesion and nasoantrostomy was carried out and histopathologic examination mainly showed a solid tumor tissue composed of odontogenic epithelium and pulp tissues admixed with dentin and enamel formation. And some part of reduced follicular epithelium of tooth germ showed a change mimicking calcifying odontogenic cyst. Taken together, we concluded the lesion is an ameloblastic fibro-odontoma with as change of calcifying odontogenic cyst.

  6. Calcified tumours of the paranasal sinuses in three horses.

    Science.gov (United States)

    Schaaf, K L; Kannegieter, N J; Lovell, D K

    2007-11-01

    Three horses, a 10-year-old Thoroughbred mare, a 9-year-old Thoroughbred gelding and a 6-year-old Arab gelding, with calcified tumours of the paranasal sinuses, are described. All horses presented with purulent nasal discharges and facial distortion. Exophthalmos, blepharospasm and ocular discharge were also a feature in individual horses. A presumptive diagnosis of a calcified tumour was made on the basis of clinical signs and radiographic and endoscopic findings. The tumours ranged from 15 to 25 cm in diameter. A large frontonasal bone flap was used to expose the tumours, which were cleaved into several pieces with an osteotome and removed. Histological examination of the masses identified cementomas in two cases and an osteoma in the third. Long term follow up from 18 months to 5 years after surgery indicated that there was no recurrence. This case series demonstrates that, although calcified tumours of the paranasal sinuses are rare in horses, they should be considered in the differential diagnosis of purulent nasal discharge, facial swelling and ocular distortion, and are amenable to surgical treatment.

  7. Net Loss of CaCO3 from a subtropical calcifying community due to seawater acidification: mesocosm-scale experimental evidence

    Directory of Open Access Journals (Sweden)

    K. S. Rodgers

    2009-08-01

    Full Text Available Acidification of seawater owing to oceanic uptake of atmospheric CO2 originating from human activities such as burning of fossil fuels and land-use changes has raised serious concerns regarding its adverse effects on corals and calcifying communities. Here we demonstrate a net loss of calcium carbonate (CaCO3 material as a result of decreased calcification and increased carbonate dissolution from replicated subtropical coral reef communities (n=3 incubated in continuous-flow mesocosms subject to future seawater conditions. The calcifying community was dominated by the coral Montipora capitata. Daily average community calcification or Net Ecosystem Calcification (NEC=CaCO3 production – dissolution was positive at 3.3 mmol CaCO3 m−2 h−1 under ambient seawater pCO2 conditions as opposed to negative at −0.04 mmol CaCO3 m−2 h−1 under seawater conditions of double the ambient pCO2. These experimental results provide support for the conclusion that some net calcifying communities could become subject to net dissolution in response to anthropogenic ocean acidification within this century. Nevertheless, individual corals remained healthy, actively calcified (albeit slower than at present rates, and deposited significant amounts of CaCO3 under the prevailing experimental seawater conditions of elevated pCO2.

  8. Salmonella enterica serovar Choleraesuis vector delivering SaoA antigen confers protection against Streptococcus suis serotypes 2 and 7 in mice and pigs.

    Science.gov (United States)

    Li, Yu-An; Ji, Zhenying; Wang, Xiaobo; Wang, Shifeng; Shi, Huoying

    2017-12-21

    Streptococcus suis is one of the major pathogens that cause economic losses in the swine industry worldwide. However, current bacterins only provide limited prophylactic protection in the field. An ideal vaccine against S. suis should protect pigs against the clinical diseases caused by multiple serotypes, or at least protect against the dominant serotype in a given geographic region. A new recombinant Salmonella enterica serotype Choleraesuis vaccine vector, rSC0011, that is based on the regulated delayed attenuation system and regulated delayed antigen synthesis system, was developed recently. In this study, an improved recombinant attenuated Salmonella Choleraesuis vector, rSC0016, was developed by incorporating a sopB mutation to ensure adequate safety and maximal immunogenicity. In the spleens of mice, rSC0016 colonized less than rSC0011. rSC0016 and rSC0011 colonized similarly in Peyer's patches of mice. The recombinant vaccine rSC0016(pS-SaoA) induced stronger cellular, humoral, and mucosal immune responses in mice and swine against SaoA, a conserved surface protein that is present in many S. suis serotypes, than did rSC0011(pS-SaoA) without sopB or rSC0018(pS-SaoA), which is an avirulent, chemically attenuated vaccine strain. rSC0016(pS-SaoA) provided 100% protection against S. suis serotype 2 in mice and pigs, and full cross-protection against SS7 in pigs. This new vaccine vector provides a foundation for the development of a universal vaccine against multiple serotypes of S. suis in pigs.

  9. Energy matrix of Sao Paulo state from 2006 to 2016; Matriz energetica do Estado de Sao Paulo 2006 a 2016

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    This report presents the economic and social energy scenarios in the state of Sao Paulo, Brazil; the analysis and projection of the energy consumption in the state concerning to industrial, residential, farming and cattle-raising, transportation, commercial, energy and public sectors. Analysis of the energy production in the state of Sao Paulo, Brazil, the production projection, the supply and consumption balance of energy is also presented.

  10. Metal and trace element sediment assessment from two estuarine systems: Santos/Sao Vicente and Cananeia, State of Sao Paulo, Brazil; Avaliacao da concentracao de metais e outros elementos de interesse em amostras de sedimentos dos estuarios de Santos/Sao Vicente e Cananeia, estado de Sao Paulo, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Amorim, Eduardo Paulo de

    2012-07-01

    This study evaluated some toxic metals such as Cd, Hg and Pb and some other major and trace elements in surface sediment samples, from two different systems under different degrees of anthropogenic actions: the estuarine system of Santos/Sao Vicente and the southern part of the Cananeia estuary, both on the Sao Paulo state coast. Sediment samples were collected in 16 stations in the Santos/Sao Vicente estuary and 13 stations in the Cananeia estuary, during summer and winter of 2005 and 2006, in both estuaries. Three analytical techniques were used: NAA, AAS and ICP OES. NAA was used for the quantification of major element concentration levels (Ca, Fe and Na), trace elements (As, Ba, Br, Cd, Co, Cu, Cr, Cs, Hf, Rb, Sb, Sc, Se, Ta, Th, U, and Zn and rare earths elements La, Ce, Eu, Nd, Sm, Lu, Tb, Yb). ICP OES was used for determination of the concentration levels of Al, Ba, Be, Bi, B, Cd, Co, Pb, Cu, Cr, Sn, Sr, Fe, Li, Mn, Mo, Ni, Tl, Ti, V and Zn. AAS for Cd and Pb quantification through graphite furnace (GF AAS) and Hg through cold vapor generation (CV AAS). Methodology validation according to precision and accuracy was performed by reference material analyses for the three analytical techniques used. Detection and quantification limits were calculated for each element evaluated. Seasonal variations (summer and winter), spatial and temporal (2005 e 2006) variations of metals and trace elements were also evaluated. In the Santos estuary, in general, metal and trace element concentrations , organic matter content and % of pelitic fraction found in the Santos channel (area 1) were higher than those of the Santos Bay (area 2) and Sao Vicente channel (area 3). Area 1 suffers high impact from industrial activities from the Cubatao region and Santos port. The sediments from station 14 (area 3, Sao Vicente channel), showed the same behavior of those from area 1, suffering influence from the industrial pole and located in a mangrove area. In comparison with TEL and PEL

  11. miR-367 regulation of DOC-2/DAB2 interactive protein promotes proliferation, migration and invasion of osteosarcoma cells.

    Science.gov (United States)

    Cai, Wei; Jiang, Haitao; Yu, Yifan; Xu, Yong; Zuo, Wenshan; Wang, Shouguo; Su, Zhen

    2017-11-01

    Recently, miR-367 is reported to exert either oncogenic or tumor suppressive effects in human malignancies. Recent study reports that miR-367 is up-regulated in OS tissues and cell lines, and abrogates adriamycin-induced apoptosis. The clinical significance of miR-367 and its function in OS need further investigation. In our study, miR-367 expression in OS was markedly elevated compared with corresponding non-tumor tissues. High miR-367 expression was associated with malignant clinical features and poor prognosis of OS patients. In accordance, the levels of miR-367 were dramatically up-regulated in OS cells. Loss of miR-367 expression in Saos-2 cells obviously inhibited the proliferation, migration and invasion of cancer cells in vitro. Meanwhile, miR-367 restoration promoted these malignant behaviors of MG-63 cells. Mechanistically, miR-367 negatively regulated DOC-2/DAB2 interactive protein (DAB2IP) abundance in OS cells. Hereby, DAB2IP was recognized as a direct target gene of miR-367 in OS. DAB2IP mRNA level was down-regulated and inversely correlated with miR-367 expression in OS specimens. DAB2IP overexpression prohibited proliferation, migration and invasion in Saos-2 cells, while DAB2IP knockdown showed promoting effects on proliferation, migration and invasion of MG-63 cells. Furthermore, the role of miR-367 might be mediated by DAB2IP-regulated phosphorylation of ERK and AKT in OS cells. To conclude, miR-367 may function as a biomarker for prediction of prognosis and a target for OS therapy. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  12. Increasing costs due to ocean acidification drives phytoplankton to be more heavily calcified: optimal growth strategy of coccolithophores.

    Directory of Open Access Journals (Sweden)

    Takahiro Irie

    Full Text Available Ocean acidification is potentially one of the greatest threats to marine ecosystems and global carbon cycling. Amongst calcifying organisms, coccolithophores have received special attention because their calcite precipitation plays a significant role in alkalinity flux to the deep ocean (i.e., inorganic carbon pump. Currently, empirical effort is devoted to evaluating the plastic responses to acidification, but evolutionary considerations are missing from this approach. We thus constructed an optimality model to evaluate the evolutionary response of coccolithophorid life history, assuming that their exoskeleton (coccolith serves to reduce the instantaneous mortality rates. Our model predicted that natural selection favors constructing more heavily calcified exoskeleton in response to increased acidification-driven costs. This counter-intuitive response occurs because the fitness benefit of choosing a better-defended, slower growth strategy in more acidic conditions, outweighs that of accelerating the cell cycle, as this occurs by producing less calcified exoskeleton. Contrary to the widely held belief, the evolutionarily optimized population can precipitate larger amounts of CaCO(3 during the bloom in more acidified seawater, depending on parameter values. These findings suggest that ocean acidification may enhance the calcification rates of marine organisms as an adaptive response, possibly accompanied by higher carbon fixation ability. Our theory also provides a compelling explanation for the multispecific fossil time-series record from ∼200 years ago to present, in which mean coccolith size has increased along with rising atmospheric CO(2 concentration.

  13. Computed Tomography Features of Benign and Malignant Calcified Thyroid Nodules: A Single-Center Study.

    Science.gov (United States)

    Kim, Donghyun; Kim, Dong Wook; Heo, Young Jin; Baek, Jin Wook; Lee, Yoo Jin; Park, Young Mi; Baek, Hye Jin; Jung, Soo Jin

    No previous studies have investigated thyroid calcification on computed tomography (CT) quantitatively by using Hounsfield unit (HU) values. This study aimed to analyze quantitative HU values of thyroid calcification on preoperative neck CT and to assess the characteristics of benign and malignant calcified thyroid nodules (CTNs). Two hundred twenty patients who underwent neck CT before thyroid surgery from January 2015 to June 2016 were included. On soft-tissue window CT images, CTNs with calcified components of 3 mm or larger in minimum diameter were included in this study. The HU values and types of CTNs were determined and analyzed. Of 61 CTNs in 49 patients, there were 42 malignant nodules and 19 benign nodules. The mean largest diameter of the calcified component was 5.3 (2.5) mm (range, 3.1-17.1 mm). A statistically significant difference was observed in the HU values of calcified portions between benign and malignant CTNs, whereas there was no significant difference in patient age or sex or in the size, location, or type of each CTN. Of the 8 CTNs with pure calcification, 3 exhibited a honeycomb pattern on bone window CT images, and these 3 CTNs were all diagnosed as papillary thyroid carcinoma on histopathological examination. Hounsfield unit values of CTNs may be helpful for differentiating malignancy from benignity.

  14. Stratigraphical analysis of the neoproterozoic sedimentary sequences of the Sao Francisco Basin

    International Nuclear Information System (INIS)

    Martins, Mariela; Lemos, Valesca Brasil

    2007-01-01

    A stratigraphic analysis was performed under the principles of Sequence Stratigraphy on the neoproterozoic sedimentary sequences of the Sao Francisco Basin (Central Brazil). Three periods of deposition separated by unconformities were recognized in the Sao Francisco Megasequence: (1) Sequences 1 and 2, a cryogenian glaciogenic sequence, followed by a distal scarp carbonate ramp, developed during stable conditions, (2) Sequence 3, a Upper Cryogenian stack homoclinal ramps with mixed carbonate-siliciclastic sedimentation, deposited under a progressive influence of compressional stresses of the Brasiliano Cycle, (3) Sequence 4, a Lower Ediacaran shallow platform dominated by siliciclastic sedimentation of molassic nature, the erosion product of the nearby uplifted thrust sheets. Each of the carbonate-bearing sequences presents a distinct δ 13 C isotopic signature. The superposition to the global curve for carbon isotopic variation allowed the recognition of a major depositional hiatus between the Paranoa and Sao Francisco Megasequences, and suggested that the glacial diamictite deposition (Jequitai Formation) took place most probably around 800 Ma. This constrains the Sao Francisco Megasequence deposition to the interval between 800 and 600 Ma (the known ages of the Brasiliano Orogeny defines the upper limit). A minor depositional hiatus (700.680 Ma) was also identified separating sequences 2 and 3. Isotopic analyses suggest that from then on, more restricted environmental conditions were established in the basin, probably associated with a first order global event, which prevailed throughout deposition of the Sequence 3. (author)

  15. Non-calcified coronary plaque volume inversely related to CD4(+) T-cell count in HIV infection.

    Science.gov (United States)

    Duarte, Horacio; Matta, Jatin R; Muldoon, Nancy; Masur, Henry; Hadigan, Colleen; Gharib, Ahmed M

    2012-01-01

    Non-calcified coronary artery plaque (NCAP) might be an important predictor of cardiovascular events; however, few studies have directly measured NCAP in HIV-infected individuals. We completed a prospective cross-sectional evaluation of NCAP and coronary calcium scores using computed tomography angiography in HIV-infected patients (n=26) without known coronary artery disease (CAD), but who had one or more CAD risk factor(s), and compared them with controls matched on age, race, sex, body mass index and Framingham Risk Score (n=26). There was no difference in coronary calcium scores (114 ± 218 versus 124 ± 298; P=0.89) or NCAP volume (65 ± 86 mm(3) versus 63 ± 82 mm(3); P=0.38) between HIV-infected patients and controls, respectively. Among HIV-infected patients, lower CD4(+) T-cell count was associated with increased NCAP volume (r=-0.52, P=0.006). The CD4(+) T-cell count remained a significant predictor of NCAP in a multivariate analysis that adjusted for age and duration of antiretroviral therapy. Plaque burden is similar between HIV-infected and uninfected individuals when matched on traditional CAD risk factors; however, immune function might mediate the development of atherosclerosis in HIV infection.

  16. Edema surrounding calcified intracranial cysticerci: clinical manifestations, natural history, and treatment

    OpenAIRE

    Nash, Theodore

    2012-01-01

    Calcified granulomas are the most common radiological finding in neurocysticercosis (10–20% of endemic populations). A small proportion serves as foci of seizure activity, which results in large numbers of persons with epilepsy. Calcified granulomas are not all the same. Some demonstrate blood–brain barrier dysfunction (magnetic resonance imaging enhancement) most likely due to the presence of inflammation, visualizable scolices, and/or gliosis. About half the patients with a recent history o...

  17. Effect of calcified plaques on estimation of arterial stenosis of lower extremity in diabetic foot patients using multislice computed tomography angiography

    International Nuclear Information System (INIS)

    Yu Xiaojing; Jin Yan; Wang Ge; Li Chunzhi; Zhang Yi; Ren Hua

    2013-01-01

    Objective: To investigate the impacts of calcified plaques on estimation of arterial stenosis of lower extremity in diabetic foot patients using 16 -slice computed tomography angiography (MSCTA). Materials and Methods: Thirty-five patients (representing 38 cases)underwent both MSCTA and digital subtraction angiography (DSA) examinations. The arteries of lower extremity were divided into 15 anatomic segments, and the degree of artery stenosis in each segment was classified as normal, mildly, moderately. severely or occluded. The extent of calcification in each segment was also assessed on cross -sectional image of MDCTA and was classified as absent, mildly, moderately, or severely. Using DSA as the standard reference, the sensitivity, specificity, accuracy, Youden index, positive predictive value and negative predictive value of MSCTA were calculated. Agreement between MSCTA and DSA was assessed by Cohen's kappa statistics. Results: In the noncalcified, mildly and moderately calcified segments of the artery above the knee, for the detection of segments that had more than mild stenosis, the sensitivity, specificity, accuracy. Youden index, positive predictive value and negative predictive value of MSCTA were 97.1%, 98.7%, 98.2%, 95.8%, 97.0% and 98.7%, respectively. In the severely calcified segments of the artery above the knee, for the detection of segments that had more than mild stenosis, the sensitivity, specificity, accuracy, Youden index, positive predictive value and negative predictive value of MSCTA were 96.3%, 93.8%, 94.7%, 90.1%, 89.7% and 97.8%, respectively. In the noncalcified, mildly and moderately calcified segments of the artery below the knee, for the detection of segments that had more than mild stenosis, the sensitivity, specificity, accuracy, Youden index, positive predictive value and negative predictive value of MSCTA were 95.1%, 93.2%, 94.1%, 88.3%, 93.4% and 94.9%, respectively. In the severely calcified segments of the artery below the

  18. Orbital Atherectomy for Treating De Novo Severely Calcified Coronary Narrowing (1-Year Results from the Pivotal ORBIT II Trial).

    Science.gov (United States)

    Généreux, Philippe; Lee, Arthur C; Kim, Christopher Y; Lee, Michael; Shlofmitz, Richard; Moses, Jeffrey W; Stone, Gregg W; Chambers, Jeff W

    2015-06-15

    Percutaneous coronary intervention of severely calcified lesions has historically been associated with major adverse cardiac event (MACE) rates as high as 30%. In the ORBIT II (Evaluate the Safety and Efficacy of OAS in Treating Severely Calcified Coronary Lesions) trial, treatment of de novo severely calcified lesions with the Diamondback 360° Coronary Orbital Atherectomy System (OAS) resulted in low rates of procedural and 30-day adverse ischemic events. The long-term results from this trial have not been reported. We sought to determine the 1-year outcomes after orbital atherectomy of severely calcified coronary lesions. ORBIT II was a single-arm trial enrolling 443 subjects at 49 US sites with severely calcified lesions usually excluded from randomized trials. OAS utilizes a centrifugal differential sanding mechanism of action for plaque modification prior to stent implantation. After OAS drug-eluting stents were implanted in 88.2% of the patients. The primary safety end point was 30-day MACE, the composite of cardiac death, myocardial infarction, or target vessel revascularization [TVR]. The present analysis reports the 1-year follow-up results from ORBIT II. One-year data were available in 433 of 443 patients (97.7%), with median follow-up time of 16.7 months. The 1-year MACE rate was 16.4%, including cardiac death (3.0%), myocardial infarction (9.7%), and target vessel revascularization (5.9%). The 1-year target lesion revascularization rate was 4.7%, and stent thrombosis occurred in 1 patient (0.2%). Independent predictors of 1-year MACE and target vessel revascularization were diameter stenosis at baseline and the use of bare-metal stents. In patients with severely calcified lesions who underwent percutaneous coronary intervention, the use of OAS was associated with low rates of 1-year adverse ischemic events compared with historical controls. This finding has important clinical implications for the selection of optimum treatment strategies for patients

  19. Calcifying/ossifying synovial sarcoma: A clinicopathologic and molecular study of 5 cases

    Directory of Open Access Journals (Sweden)

    Nasir Ud Din

    2015-01-01

    Full Text Available Background: Synovial sarcoma (SS is a soft tissue sarcoma with a generally aggressive behavior. Calcifying/ossifying SS is a rare variant associated with a favorable prognosis. Aim: The aim was to report clinicopathological features and molecular analysis of 5 cases of calcifying/ossifying SS. Materials and Methods: Record of 370 cases of SS reported in the section of Histopathology, of a tertiary care Hospital, between 2002 and 2011 were retrieved. Five cases exhibiting extensive calcification and ossification were identified. Immunohistochemistry was performed using Flex technique. Molecular analysis of these 5 cases was performed later at the collaborative Hospital abroad, by reverse transcription polymerase chain reaction. Results and Conclusions: The ages of the patients ranged from 13 to 44 years (mean age 27 years; female to male ratio 1.6:1. The duration of symptoms ranged from 5 months to 5 years. Histologically, 4 were monophasic, and 1 was biphasic. Three cases exhibited extensive calcification and two extensive ossification. Immunohistochemical stain (epithelial membrane antigen was positive in all 5 cases, CKAE1/AE3 (3/4, Bcl2 (4/4, S100 (4/4, CK7 (2/2, CD99 (1/3 and vimentin (2/2. Intact RNA was obtained from 3 cases, all of which were positive for the SYT/SSX fusion transcript. Follow-up was available in 4 cases and ranged from 19 months to 85 months (mean 50 months. Local recurrence was seen in 2 cases. In conclusions, we report clinicopathologic features of 5 cases of calcifying/ossifying SS. The duration of symptoms and mean age of patients is similar to the literature. A slight female predominance was seen in contrast to a male predominance described in the literature. The clinical course of our cases validates the favorable prognosis of this rare type of SS.

  20. Energy statistical yearbook by municipalities of Sao Paulo state - 2015: calendar year 2014; Anuario estatistico de energeticos por municipio no estado de Sao Paulo - 2015: ano base 2014

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-07-01

    The Government of the Sao Paulo state, Brazil, presents the {sup S}tatistical Yearbook of Energy by Municipality in the State of Sao Paulo in 2015 {sup -} calendar year 2014, prepared by Power State Department. The report provides consolidated data on the primary energy consumed by 645 municipalities - electricity, ethanol, petroleum products and natural gas, as well as their carbon dioxide (CO{sub 2}). It is about the production of strategic information to the municipalities together with the State Government to plan its priorities in energy and services.

  1. The Gene Ontology (GO) Cellular Component Ontology: integration with SAO (Subcellular Anatomy Ontology) and other recent developments

    Science.gov (United States)

    2013-01-01

    Background The Gene Ontology (GO) (http://www.geneontology.org/) contains a set of terms for describing the activity and actions of gene products across all kingdoms of life. Each of these activities is executed in a location within a cell or in the vicinity of a cell. In order to capture this context, the GO includes a sub-ontology called the Cellular Component (CC) ontology (GO-CCO). The primary use of this ontology is for GO annotation, but it has also been used for phenotype annotation, and for the annotation of images. Another ontology with similar scope to the GO-CCO is the Subcellular Anatomy Ontology (SAO), part of the Neuroscience Information Framework Standard (NIFSTD) suite of ontologies. The SAO also covers cell components, but in the domain of neuroscience. Description Recently, the GO-CCO was enriched in content and links to the Biological Process and Molecular Function branches of GO as well as to other ontologies. This was achieved in several ways. We carried out an amalgamation of SAO terms with GO-CCO ones; as a result, nearly 100 new neuroscience-related terms were added to the GO. The GO-CCO also contains relationships to GO Biological Process and Molecular Function terms, as well as connecting to external ontologies such as the Cell Ontology (CL). Terms representing protein complexes in the Protein Ontology (PRO) reference GO-CCO terms for their species-generic counterparts. GO-CCO terms can also be used to search a variety of databases. Conclusions In this publication we provide an overview of the GO-CCO, its overall design, and some recent extensions that make use of additional spatial information. One of the most recent developments of the GO-CCO was the merging in of the SAO, resulting in a single unified ontology designed to serve the needs of GO annotators as well as the specific needs of the neuroscience community. PMID:24093723

  2. Aggressive plaque modification with rotational atherectomy and cutting balloon for optimal stent expansion in calcified lesions

    Science.gov (United States)

    Tang, Zhe; Bai, Jing; Su, Shao-Ping; Lee, Pui-Wai; Peng, Liang; Zhang, Tao; Sun, Ting; Nong, Jing-Guo; Li, Tian-De; Wang, Yu

    2016-01-01

    Objective To evaluate the factors affecting optimal stent expansion in calcified lesions treated by aggressive plaque modification with rotational atherectomy (RA) and a cutting balloon (CB). Methods From January 2014 to May 2015, 92 patients with moderate to severe coronary calcified lesions underwent rotational atherectomy and intravascular ultrasound imaging at Chinese PLA General Hospital (Beijing, China) were included in this study. They were divided into a rotational artherectomy combined with cutting balloon (RACB) group (46 patients treated with RA followed by CB angioplasty) and an RA group (46 patients treated with RA followed by plain balloon angioplasty). Another 40 patients with similar severity of their calcified lesions treated with plain old balloon angioplasty (POBA) were demographically matched to the other groups and defined as the POBA group. All patients received a drug-eluting stent after plaque preparation. Lumen diameter and lumen diameter stenosis (LDS) were measured by quantitative coronary angiography at baseline, after RA, after dilatation, and after stenting. Optimal stent expansion was defined as the final LDS < 10%. Results The initial and post-RA LDS values were similar among the three groups. However, after dilatation, the LDS significantly decreased in the RACB group (from 54.5% ± 8.9% to 36.1% ± 7.1%) but only moderately decreased (from 55.7% ± 7.8% to 46.9% ± 9.4%) in the RA group (time × group, P < 0.001). After stenting, there was a higher rate of optimal stent expansion in the RACB group (71.7% in the RACB group, 54.5% in the RA group, and 15% in the POBA group, P < 0.001), and the final LDS was significantly diminished in the RACB group compared to the other two groups (6.0% ± 2.3%, 10.8% ± 3.3%, 12.7% ± 2.1%, P < 0.001). Moreover, an LDS ≤ 40% after plaque preparation (OR = 2.994, 95% CI: 1.297–6.911) was associated with optimal stent expansion, which also had a positive correlation with the appearance of a

  3. The calcified lung nodule: What does it mean?

    Directory of Open Access Journals (Sweden)

    Khan Ali

    2010-01-01

    Full Text Available The aim of this review is to present a pictorial essay emphasizing the various patterns of calcification in pulmonary nodules (PN to aid diagnosis and to discuss the differential diagnosis and the pathogenesis where it is known. The imaging evaluation of PN is based on clinical history, size, distribution and the gross appearance of the nodule as well as feasibility of obtaining a tissue diagnosis. Imaging is instrumental in the management of PN and one should strive not only to identify small malignant tumors with high survival rates but to spare patients with benign PN from undergoing unnecessary surgery. The review emphasizes how to achieve these goals. One of the most reliable imaging features of a benign lesion is a benign pattern of calcification and periodic follow-up with computed tomography showing no growth for 2 years. Calcification in PN is generally considered as a pointer toward a possible benign disease. However, as we show here, calcification in PN as a criterion to determine benign nature is fallacious and can be misleading. The differential considerations of a calcified lesion include calcified granuloma, hamartoma, carcinoid, osteosarcoma, chondrosarcoma and lung metastases or a primary bronchogenic carcinoma among others. We describe and illustrate different patterns of calcification as seen in PN on imaging.

  4. New aerosol particles formation in the Sao Paulo Metropolitan Area

    Science.gov (United States)

    Vela, Angel; Andrade, Maria de Fatima; Ynoue, Rita

    2016-04-01

    The Sao Paulo Metropolitan Area (SPMA), in the southeast region of Brazil, is considered a megalopolis comprised of Sao Paulo city and more 38 municipalities. The air pollutant emissions in the SPMA are related to the burning of the fuels: etanol, gasohol (gasoline with 25% ethanol) and diesel. According to CETESB (2013), the road vehicles contributed up to about 97, 87, and 80% of CO, VOCs and NOx emissions in 2012, respectively, being most of NOx associated to diesel combustion and most of CO and VOCs from gasohol and ethanol combustion. Studies conducted on ambient air pollution in the SPMA have shown that black carbon (BC) explains 21% of mass concentration of PM2.5 compared with 40% of organic carbon (OC), 20% of sulfates, and 12% of soil dust (Andrade et al., 2012). Most of the observed ambient PM2.5 mass concentration usually originates from precursors gases such as sulphur dioxide (SO2), ammonia (NH3), nitrogen oxides (NOx) and VOCs as well as through the physico-chemical processes such as the oxidation of low volatile hydrocarbons transferring to the condensed phase (McMurry et al., 2004). The Weather Research and Forecasting with Chemistry model (WRF-Chem; Grell et al. 2005), configured with three nested grid cells: 75, 15, and 3 km, is used as photochemical modeling to describe the physico-chemical processes leading to evolution of particles number and mass size distribution from a vehicular emission model developed by the IAG-USP laboratory of Atmospheric Processes and based on statistical information of vehicular activity. The spatial and temporal distributions of emissions in the finest grid cell are based on road density products compiled by the OpenStreetMap project and measurements performed inside tunnels in the SPMA, respectively. WRF-Chem simulation with coupled primary aerosol (dust and sea-salt) and biogenic emission modules and aerosol radiative effects turned on is conducted as the baseline simulation (Case_0) to evaluate the model

  5. Predominance of heavily calcified coccolithophores at low CaCO3 saturation during winter in the Bay of Biscay

    OpenAIRE

    Smith, Helen E. K.; Tyrrell, Toby; Charalampopoulou, Anastasia; Dumousseaud, Cynthia; Legge, Oliver J.; Birchenough, Sarah; Pettit, Laura R.; Garley, Rebecca; Hartman, Sue E.; Hartman, Mark C.; Sagoo, Navjit; Daniels, Chris J.; Achterberg, Eric P.; Hydes, David J.

    2012-01-01

    Coccolithophores are an important component of the Earth system, and, as calcifiers, their possible susceptibility to ocean acidification is of major concern. Laboratory studies at enhanced pCO2 levels have produced divergent results without overall consensus. However, it has been predicted from these studies that, although calcification may not be depressed in all species, acidification will produce “a transition in dominance from more to less heavily calcified coccolithophores” [Ridgwell A,...

  6. Transcatheter closure of calcified patent ductus arteriosus in older adult patients: Immediate and 12-month follow-up results.

    Science.gov (United States)

    Gu, Xinghua; Zhang, Qiuwang; Sun, Hourong; Fei, Jianchun; Zhang, Xiquan; Kutryk, Michael J

    2017-05-01

    To present our experience in transcatheter closure of calcified patent ductus arteriosus (PDA) in older adult patients, which has rarely been reported. From 2009 to 2014, a total of 16 patients (median age 58 years) with calcified PDA underwent transcatheter closure in our center. All patients were symptomatic with major symptoms being exertional dyspnea (in 12), palpitations (in 8), and fatigue (in 5). A continuous murmur was heard in all patients. The median ductus diameter was 4 mm (range 3-7 mm). The median Qp/Qs was 1.6 (range 1.4-2.9). Transcatheter closure was performed for all patients. The size of the occluder selected was 2-3 mm greater than the narrowest portion of PDA. We experienced difficulties in advancing the multipurpose catheter through the calcified duct in about one third of patients (5/16). Considering that calcified tissue has a greater tendency to rupture, hence, to close PDA in these patients, they adopted the retrograde wire-assisted technique and modified the procedure to reduce the shear stress of sheath and avoid any sheath kinking. For the remaining 11 patients, the advancement of the multipurpose catheter through the calcified duct was smooth and the conventional antegrade approach was applied. Clinical examination, standard 12-lead electrocardiography, chest x-ray, and transthoracic echocardiography were performed before hospital discharge, at 1-, 3-, 6-, and 12-months follow-ups. All PDAs were successfully closed. There were no deaths. Three patients had a trivial residual shunt, with one also having intravascular hemolysis. Following pharmacological treatment, hemolysis signs vanished at 7 days postprocedure. The trivial residual shunt disappeared in all three patients at 3-month follow-up. No new-onset residual shunt, device embolization, device dislocation, infective endocarditis, or embolism was observed at all follow-up time points. Successful closure of calcified PDA with few complications in older adult patients was achieved

  7. Cell adhesion on nanotextured slippery superhydrophobic substrates.

    Science.gov (United States)

    Di Mundo, Rosa; Nardulli, Marina; Milella, Antonella; Favia, Pietro; d'Agostino, Riccardo; Gristina, Roberto

    2011-04-19

    In this work, the response of Saos2 cells to polymeric surfaces with different roughness/density of nanometric dots produced by a tailored plasma-etching process has been studied. Topographical features have been evaluated by atomic force microscopy, while wetting behavior, in terms of water-surface adhesion energy, has been evaluated by measurements of drop sliding angle. Saos2 cytocompatibility has been investigated by scanning electron microscopy, fluorescent microscopy, and optical microscopy. The similarity in outer chemical composition has allowed isolation of the impact of the topographical features on cellular behavior. The results indicate that Saos2 cells respond differently to surfaces with different nanoscale topographical features, clearly showing a certain inhibition in cell adhesion when the nanoscale is particularly small. This effect appears to be attenuated in surfaces with relatively bigger nanofeatures, though these express a more pronounced slippery/dry wetting character. © 2011 American Chemical Society

  8. Operation auxiliary system (SAO)

    International Nuclear Information System (INIS)

    Lolich, J.; Santome, D.; Drexler, J.

    1990-01-01

    This work presents an auxiliary system for nuclear power plants operation (SAO). The development purpose consisted in a computing supervision system to be installed at different sites of a reactor, mainly in the control room. The inclusion of this system to a nuclear power plant minimizes the possibility of human error for the facility operation. (Author) [es

  9. Calcified neurofibroma at the masticator space

    International Nuclear Information System (INIS)

    Fuster, M. J.; Saez, J.; Alonso, S.; Fernandez, F.

    1999-01-01

    Odontogenic infections and invasive tumors or surrounding spaces are the main causes of lesions in the masticator space (MS). Primary tumors, including neurofibromas, are very uncommon within this compartment. We present a calcified neurofibroma at the masticator space. We emphasize the importance of CT to locate lesions at different neck spaces. This method allows to restraint differential diagnoses and to plan surgical approach. (Author) 8 refs

  10. Exercise does not affect stiffness and mineralisation of third metacarpal condylar subarticular calcified tissues in 2 year old thoroughbred racehorses

    Directory of Open Access Journals (Sweden)

    VL Ferguson

    2008-09-01

    Full Text Available Impact exercise has a profound effect in increasing volumetric density of epiphyseal bone, as clearly shown in 2 year old thoroughbred racehorses from which we derived the tissue studied in the present investigation. Here, we asked the question whether the fabric-level properties of the mineralised tissues immediately below hyaline articular cartilage which transmit the extra loads are themselves altered in consequence. We therefore studied the nanoindentation elastic modulus and its relationship to the concentration of mineral determined by quantitative backscattered electron imaging in the heavily loaded palmar medial and lateral condyles of the distal third metacarpal bone (Mc3 of 4 untrained and 4 trained 2-year old Thoroughbred racehorses. We found no difference between trained and untrained horses in either subchondral bone or calcified cartilage in the mean stiffness or mineral content or their correlation. Thus neither articular calcified cartilage nor the immediately adjacent subchondral bone were affected by exercise, even though they transmitted the higher load associated with athletic training through to the deeper bone, which itself responded floridly to exercise. Under the circumstances of this experiment and at least in the very small regions studied, therefore, the structure of these two tissues was apparently optimised to function.

  11. Detection of Repair of the Zone of Calcified Cartilage with Osteoarthritis through Mesenchymal Stem Cells by Ultrashort Echo Time Magnetic Resonance Imaging.

    Science.gov (United States)

    Zhou, Quan; Li, Shao-Lin; Ma, Ya-Jun; de Tal, Vicki; Li, Wei; Zhao, Ying-Hua

    2018-05-05

    Currently, magnetic resonance imaging (MRI) is the most commonly used imaging modality for observing the growth and development of mesenchymal stem cells (MSCs) after in vivo transplantation to treat osteoarthritis (OA). However, it is a challenge to accurately monitor the treatment effects of MSCs in the zone of calcified cartilage (ZCC) with OA. This is especially true in the physiological and biochemical views that are not accurately detected by MRI contrast agents. In contrast, ultrashort time echo (UTE) MRI has been shown to be sensitive to the presence of the ZCC, creating the potential for more effectively observing the repair of the ZCC in OA by MSCs. A special focus is given to the outlook of the use of UTE MRI to detect repair of the ZCC with OA through MSCs. The limitations of the current techniques for clinical applications and future directions are also discussed. Using the combined keywords: "osteoarthritis", "mesenchymal stem cells", "calcified cartilage", and "magnetic resonance imaging", the PubMed/MEDLINE literature search was conducted up to June 1, 2017. A total of 132 published articles were initially identified citations. Of the 132 articles, 48 articles were selected after further detailed review. This study referred to all the important English literature in full. In contrast, UTE MRI has been shown to be sensitive to the presence of the ZCC, creating the potential for more effectively observing the repair of the ZCC in OA by MSCs. The current studies showed that the ZCC could be described in terms of its histomorphology and biochemistry by UTE MRI. We prospected that UTE MRI has been shown the potential for more effectively observing the repair of the ZCC in OA by MSCs in vivo.

  12. Research on DNA methylation of human osteosarcoma cell MGMT and its relationship with cell resistance to alkylating agents.

    Science.gov (United States)

    Guo, Jun; Cui, Qiu; Jiang, WeiHao; Liu, Cheng; Li, DingFeng; Zeng, Yanjun

    2013-08-01

    The objective of this study was to explore the O(6)-methylguanine-DNA methyltransferase (MGMT) gene methylation status and its protein expression, as well as the effects of demethylating agent 5-Aza-2'-deoxycytidine (5-Aza-CdR) on MGMT gene expression and its resistance to alkylating agents, and to elucidate MGMT expression mechanism and significance in osteosarcoma. The human osteosarcoma cell lines Saos-2 and MG-63 were collected and treated with 5-Aza-CdR for 6 days. The cells not treated with 5-Aza-CdR were set as a negative control. The genomic DNA was extracted from the Saos-2 and MG-63 cells using methylation-specific PCR to detect the promoter CpG island methylation status of the MGMT gene. Cell sensitivity to alkylating agents before and after drug administration was detected by the MTT method. The variation in MGMT gene mRNA and protein was detected by reverse transcription PCR (RT-PCR) and Western blotting. The MGMT promoter gene of normal Saos-2 cells was methylated, with reduced MGMT mRNA and protein expression; the MGMT mRNA and protein expression of Saos-2 cells treated with 5-Aza-CdR was obviously enhanced, and its sensitivity to alkylating agents was reversed. Meanwhile, with promoter CpG island unmethylation of the MGMT gene, MGMT protein was expressed in the normal MG-63 cells and the MG-63 cells treated with 5-Aza-CdR, and both showed resistance to alkylating agents. The methylation status of the MGMT gene promoter in human osteosarcoma cells reflected the cells' ability to induce MGMT protein expression and can be used as a molecular marker to project the sensitivity of cancer tissues to alkylating agent drugs.

  13. Percutaneous Coronary Intervention in Severely Calcified Unprotected Left Main Coronary Artery Disease: Initial Experience With Orbital Atherectomy.

    Science.gov (United States)

    Lee, Michael S; Shlofmitz, Evan; Kaplan, Barry; Shlofmitz, Richard

    2016-04-01

    We report the clinical outcomes of patients who underwent percutaneous coronary intervention (PCI) with orbital atherectomy for severely calcified unprotected left main coronary artery (ULMCA) disease. Although surgical revascularization is the gold standard for patients with ULMCA disease, not all patients are candidates for this. PCI is increasingly used to treat complex coronary artery disease, including ULMCA disease. The presence of severely calcified lesions increases the complexity of PCI. Orbital atherectomy can be used to facilitate stent delivery and expansion in severely calcified lesions. The clinical outcomes of patients treated with orbital atherectomy for severely calcified ULMCA disease have not been reported. From May 2014 to July 2015, a total of 14 patients who underwent PCI with orbital atherectomy for ULMCA disease were retrospectively evaluated. The primary endpoint was major cardiac and cerebrovascular event (cardiac death, myocardial infarction, stroke, and target-lesion revascularization) at 30 days. The mean age was 78.2 ± 5.8 years. The mean ejection fraction was 41.8 ± 19.8%. Distal bifurcation disease was present in 9 of 14 patients. Procedural success was achieved in all 14 patients. The 30-day major adverse cardiac and cerebrovascular event rate was 0%. One patient had coronary dissection that was successfully treated with stenting. No patient had perforation, slow flow, or thrombosis. Orbital atherectomy in patients with severely calcified ULMCA disease is feasible, even in high-risk patients who were considered poor surgical candidates. Randomized trials are needed to determine the role of orbital atherectomy in ULMCA disease.

  14. Calcifying Aponeurotic Fibroma with Osseous Involvement of the Finger: a Case Report with Radiologic and US Findings

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Soo Jung; Ahn, Jae Hong; Kang, Gil Hyun; Lee, Jong Hyeog; Park, Man Soo; Ryu, Dae Sik; Jung, Seung Moon [GangNeung Asan Hospital, University of Ulsan College of Medicine, Gangneung (Korea, Republic of)

    2008-02-15

    Calcifying aponeurotic fibroma is a rare soft tissue tumor that occurs in the distal extremities of children and adolescents. We report ultrasound and X-ray findings of a calcifying aponeurotic fibroma in the finger of a 36-year-old woman, associated with distal phalangeal bone involvement. Calcifying aponeurotic fibroma is a rare, locally aggressive fibroblastic lesion occurring primarily in the palms of the hands and soles of the feet in young children and adolescents under 20 years of age. Clinical presentation is a unique, hard, and painless palpable mass. This soft tissue tumor typically infiltrates into the surrounding fascia or muscle and has a predilection for recurrence after surgical removal. However, bone involvement in calcifying aponeurotic fibroma is a very rare condition and we found only three pediatric cases in the literature. We present the ultrasound and radiographic findings of a calcifying aponeurotic fibroma in the finger of a 36-year-old woman, associated with erosive bone destruction of the distal phalanx. In summary, calcifying aponeurotic fibroma is a rare soft tissue tumor that presents as a painless mass primarily on the volar surface of the hands and plantar aspects of the feet in juveniles, but this tumor should be also included in differential diagnoses of any mass with calcification and adjacent bone involvement in the distal phalanx of the finger. In addition, US could be useful for the preoperative evaluation of digital calcifying aponeurotic fibroma.

  15. Calcifying Aponeurotic Fibroma with Osseous Involvement of the Finger: a Case Report with Radiologic and US Findings

    International Nuclear Information System (INIS)

    Choi, Soo Jung; Ahn, Jae Hong; Kang, Gil Hyun; Lee, Jong Hyeog; Park, Man Soo; Ryu, Dae Sik; Jung, Seung Moon

    2008-01-01

    Calcifying aponeurotic fibroma is a rare soft tissue tumor that occurs in the distal extremities of children and adolescents. We report ultrasound and X-ray findings of a calcifying aponeurotic fibroma in the finger of a 36-year-old woman, associated with distal phalangeal bone involvement. Calcifying aponeurotic fibroma is a rare, locally aggressive fibroblastic lesion occurring primarily in the palms of the hands and soles of the feet in young children and adolescents under 20 years of age. Clinical presentation is a unique, hard, and painless palpable mass. This soft tissue tumor typically infiltrates into the surrounding fascia or muscle and has a predilection for recurrence after surgical removal. However, bone involvement in calcifying aponeurotic fibroma is a very rare condition and we found only three pediatric cases in the literature. We present the ultrasound and radiographic findings of a calcifying aponeurotic fibroma in the finger of a 36-year-old woman, associated with erosive bone destruction of the distal phalanx. In summary, calcifying aponeurotic fibroma is a rare soft tissue tumor that presents as a painless mass primarily on the volar surface of the hands and plantar aspects of the feet in juveniles, but this tumor should be also included in differential diagnoses of any mass with calcification and adjacent bone involvement in the distal phalanx of the finger. In addition, US could be useful for the preoperative evaluation of digital calcifying aponeurotic fibroma

  16. Association Between Osteogenesis and Inflammation During the Progression of Calcified Plaque Evaluated by 18F-Fluoride and 18F-FDG.

    Science.gov (United States)

    Li, Xiang; Heber, Daniel; Cal-Gonzalez, Jacobo; Karanikas, Georgios; Mayerhoefer, Marius E; Rasul, Sazan; Beitzke, Dietrich; Zhang, Xiaoli; Agis, Hermine; Mitterhauser, Markus; Wadsak, Wolfgang; Beyer, Thomas; Loewe, Christian; Hacker, Marcus

    2017-06-01

    18 F-FDG is the most widely validated PET tracer for the evaluation of atherosclerotic inflammation. Recently, 18 F-NaF has also been considered a potential novel biomarker of osteogenesis in atherosclerosis. We aimed to analyze the association between inflammation and osteogenesis at different stages of atherosclerosis, as well as the interrelationship between these 2 processes during disease progression. Methods: Thirty-four myeloma patients underwent 18 F-NaF and 18 F-FDG PET/CT examinations. Lesions were divided into 3 groups (noncalcified, mildly calcified, and severely calcified lesions) on the basis of calcium density as measured in Hounsfield units by CT. Tissue-to-background ratios were determined from PET for both tracers. The association between inflammation and osteogenesis during atherosclerosis progression was evaluated in 19 patients who had at least 2 examinations with both tracers. Results: There were significant correlations between the maximum tissue-to-background ratios of the 2 tracers (Spearman r = 0.5 [ P < 0.01]; Pearson r = 0.4 [ P < 0.01]) in the 221 lesions at baseline. The highest uptake of both tracers was observed in noncalcified lesions, but without any correlation between the tracers (Pearson r = 0.06; P = 0.76). Compared with noncalcified plaques, mildly calcified plaques showed concordant significantly lower accumulation, with good correlation between the tracers (Pearson r = 0.7; P < 0.01). In addition, enhanced osteogenesis-derived 18 F-NaF uptake and regressive inflammation-derived 18 F-FDG uptake were observed in severely calcified lesions (Pearson r = 0.4; P < 0.01). During follow-up, increased calcium density and increased mean 18 F-NaF uptake were observed, whereas mean 18 F-FDG uptake decreased. Most noncalcified (86%) and mildly calcified (81%) lesions and 47% of severely calcified lesions had concordant development of both vascular inflammation and osteogenesis. Conclusion: The combination of 18 F-NaF PET imaging and 18 F

  17. Case report - calcification of the medial collateral ligament of the knee with simultaneous calcifying tendinitis of the rotator cuff.

    Science.gov (United States)

    Kamawal, Yama; Steinert, Andre F; Holzapfel, Boris M; Rudert, Maximilian; Barthel, Thomas

    2016-07-13

    Calcification of the medial collateral ligament (MCL) of the knee is a very rare disease. We report on a case of a patient with a calcifying lesion within the MCL and simultaneous calcifying tendinitis of the rotator cuff in both shoulders. Calcification of the MCL was diagnosed both via x-ray and magnetic resonance imaging (MRI) and was successfully treated surgically. Calcifying tendinitis of the rotator cuff was successfully treated applying conservative methods. This is the first case report of a patient suffering from both a calcifying lesion within the medial collateral ligament and calcifying tendinitis of the rotator cuff in both shoulders. Clinical symptoms, radio-morphological characteristics and macroscopic features were very similar and therefore it can be postulated that the underlying pathophysiology is the same in both diseases. Our experience suggests that magnetic resonance imaging and x-ray are invaluable tools for the diagnosis of this inflammatory calcifying disease of the ligament, and that surgical repair provides a good outcome if conservative treatment fails. It seems that calcification of the MCL is more likely to require surgery than calcifying tendinitis of the rotator cuff. However, the exact reason for this remains unclear to date.

  18. ARTHROSCOPIC TREATMENT OF CALCIFYING TENDINITIS OF THE ROTATOR CUFF.

    Science.gov (United States)

    Neto, Arnaldo Amado Ferreira; Trevizani, Cassio Silva; Benegas, Eduardo; Malavolta, Eduardo Angeli; Gracitelli, Mauro Emílio Conforto; Bitar, Alexandre Carneiro; Neto, Francisco José Dos Santos

    2010-01-01

    To evaluate the clinical and radiographic results from arthroscopic surgical treatment of the rotator cuff in patients with calcifying tendinitis. A retrospective study was conducted on twenty patients who underwent arthroscopic treatment for calcifying tendinitis of the shoulder between March 1999 and November 2005. Six patients were excluded due to loss of follow-up. The average follow-up period was 41.4 months. Eight patients (57%) were female and six (43%) were male. The right side was affected in 10 cases (71%) and the left in four cases (29%). Nine cases (64%) had calcification in the supraspinatus tendon, two (14%) in the infraspinatus tendon, and three (21%) in both tendons. In all cases, resection of the calcium deposits was performed by means of a needle (Jelco® No. 14) in combination with curettage (mini-curette). Two shoulders (14%) underwent subacromial decompression, and one (7%) underwent excision of the distal clavicle. A tendon-tendon suture was performed in three shoulders (21%). None of the patients underwent tendon-bone reinsertion. The mean score obtained on the UCLA scale was 33 points (26-35), thus indicating that a majority of patients had good results. In the final radiographic evaluation, none of the patients showed signs of calcification. Arthroscopic treatment of calcifying tendinitis of the shoulder safely allows excision of the calcification, leading to good results in relation to shoulder pain and function.

  19. Calcified trichinosis of pectoral muscle: mammographic appearance

    Energy Technology Data Exchange (ETDEWEB)

    Apesteguia, L. [Servicio de Radiologia, Hospital Virgen del Camino, Pamplona (Spain); Murillo, A. [Servicio de Radiologia, Hospital Virgen del Camino, Pamplona (Spain); Biurrun, J. [Servicio de Radiologia, Hospital Virgen del Camino, Pamplona (Spain); Garcia-Rostan, G. [Servicio Anatomia Patologica, Hospital Virgen del Camino, Pamplona (Spain); Reta, A. [Servicio de Analisis Clinicos, Hospital Virgen del Camino, Pamplona (Spain); Dominguez, F. [Servicio Cirugia, Hospital Virgen del Camino, Pamplona (Spain)

    1995-12-31

    By mammographic screening we had detected six asymptomatic women who showed numerious tiny and well-delineated round to ovoid microcalcification superimposed on pectoral shadows, in the oblique medio-lateral (OML) view. Our objective was to achieve a better evaluation of these calcifications and investigate their origin. Magnified mammograms of pectoral muscles were done in the six women. A questionnaire concerning the patients` diets was also administered. Trichinella antibody titres were quantified by sero-agglutination. Microcalcifications within pectoral muscle fibres were demonstrated in all the cases. Five women admitted to having eaten home-made pork products in the past. One of them showed a slightly elevated antibody titre. We confirmed the suspected diagnosis of calcified trichinosis by a surgical biopsy of the pectoral muscle performed on one of the patients. We conclude that chronic calcified trichinosis of the pectoral muscle can be visualised in the OML view of a conventional mammogram. The mammographic appearance of this entity is very characteristic and biopsy would not be required for its diagnosis in the future. (orig.)

  20. Calcified trichinosis of pectoral muscle: mammographic appearance

    International Nuclear Information System (INIS)

    Apesteguia, L.; Murillo, A.; Biurrun, J.; Garcia-Rostan, G.; Reta, A.; Dominguez, F.

    1995-01-01

    By mammographic screening we had detected six asymptomatic women who showed numerious tiny and well-delineated round to ovoid microcalcification superimposed on pectoral shadows, in the oblique medio-lateral (OML) view. Our objective was to achieve a better evaluation of these calcifications and investigate their origin. Magnified mammograms of pectoral muscles were done in the six women. A questionnaire concerning the patients' diets was also administered. Trichinella antibody titres were quantified by sero-agglutination. Microcalcifications within pectoral muscle fibres were demonstrated in all the cases. Five women admitted to having eaten home-made pork products in the past. One of them showed a slightly elevated antibody titre. We confirmed the suspected diagnosis of calcified trichinosis by a surgical biopsy of the pectoral muscle performed on one of the patients. We conclude that chronic calcified trichinosis of the pectoral muscle can be visualised in the OML view of a conventional mammogram. The mammographic appearance of this entity is very characteristic and biopsy would not be required for its diagnosis in the future. (orig.)

  1. Orbital atherectomy system in treating calcified coronary lesions: 3-Year follow-up in first human use study (ORBIT I trial)

    Energy Technology Data Exchange (ETDEWEB)

    Bhatt, Parloop, E-mail: parloop.bhatt@cims.me [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India); Parikh, Parth, E-mail: parth.parikh@cimshospital.org [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India); Patel, Apurva, E-mail: patela12@ccf.org [Internal Medicine, Cleveland Clinic Foundation, Cleveland, OH (United States); Chag, Milan, E-mail: milan.chag@cims.me [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India); Chandarana, Anish, E-mail: anish.chandarana@cims.me [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India); Parikh, Roosha, E-mail: parikhr@ccf.org [Internal Medicine, Cleveland Clinic Foundation, Cleveland, OH (United States); Parikh, Keyur, E-mail: keyur.parikh@cims.me [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India)

    2014-06-15

    Background/Purpose: The ORBIT I trial evaluated the safety and performance of an orbital atherectomy system (OAS) in treating de novo calcified coronary lesions. Severely calcified coronary arteries pose ongoing treatment challenges. Stent placement in calcified lesions can result in stent under expansion, malapposition and procedural complications. OAS treatment may be recommended to facilitate coronary stent implantation in these difficult lesions. Materials/Methods: Fifty patients with de novo calcified coronary lesions were enrolled in the ORBIT I trial. Patients were treated with the OAS followed by stent placement. Our institution treated 33/50 patients and continued follow-up for 3 years. Results: Average age was 54.4 years and 90.9% were males. Mean lesion length was 15.9 mm. The average number of OAS devices used per patient was 1.3. Procedural success was achieved in 97% of patients. Angiographic complications were observed in five patients (two minor dissections, one major dissection and two perforations). The cumulative major adverse cardiac event (MACE) rate was 6.1% in-hospital, 9.1% at 30 days, 12.1% at 6 months, 15.2% at 2 years, and 18.2% at 3 years. The MACE rate included two in-hospital non Q-wave myocardial infarctions (MI), one additional non Q-wave MI at 30 days leading to target lesion revascularization (TLR), and three cardiac deaths. Conclusions: The ORBIT I trial suggests that OAS treatment may offer an effective method to modify calcified coronary lesion compliance to facilitate optimal stent placement in these difficult-to-treat patients with acceptable levels of safety up to 3 years post-index procedure.

  2. Discovery of the mineral brucite (magnesium hydroxide) in the tropical calcifying alga Polystrata dura (Peyssonneliales, Rhodophyta).

    Science.gov (United States)

    Nash, Merinda C; Russell, Bayden D; Dixon, Kyatt R; Liu, Minglu; Xu, Huifang

    2015-06-01

    Red algae of the family Peyssonneliaceae typically form thin crusts impregnated with aragonite. Here, we report the first discovery of brucite in a thick red algal crust (~1 cm) formed by the peyssonnelioid species Polystrata dura from Papua New Guinea. Cells of P. dura were found to be infilled by the magnesium-rich mineral brucite [Mg(OH)2 ]; minor amounts of magnesite and calcite were also detected. We propose that cell infill may be associated with the development of thick (> ~5 mm) calcified red algal crusts, integral components of tropical biotic reefs. If brucite infill within the P. dura crust enhances resistance to dissolution similarly to crustose coralline algae that infill with dolomite, then these crusts would be more resilient to future ocean acidification than crusts without infill. © 2015 Phycological Society of America.

  3. Results of Occupational Monitoring Program in Technologic Navy Center in Sao Paulo

    International Nuclear Information System (INIS)

    Perez, Clarice F.A.; Bitelli, Ricardo D.; Oliveira, Rodemir

    2013-01-01

    The CTMSP, Centro Tecnologico da Marinha em Sao Paulo, is a nuclear research Center located in the State of Sao Paulo. CTMSP headquarters is placed at the Campus of the University of Sao Paulo. Most of CTMSP nuclear facilities are at the Centro Experimental Aramar (CEA), located 120 km northwest from the Capital city of Sao Paulo. CTMSP has two major nuclear programs. One is the development of a PWR reactor for naval propulsion, and the other is associated with the front end fuel cycle technology comprising enrichment, conversion and reconversion. These activities require an extensive area monitoring program. This paper presents the results of this program covering the years of 2003 to 2011. The results include surface monitoring, dose rates and uranium concentrations in the air. (author)

  4. Atmospheric alcohols and aldehydes concentrations measured in Osaka, Japan and in Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Nguyen, Ha Thi-Hoang; Takenaka, Norimichi; Bandow, Hiroshi; Maeda, Yasuaki; Oliva, S.T. de; Botelho, M.M.; Tavares, T.M.

    2001-01-01

    The use of alcohol fuel has received much attention since the 1980s. In Brazil, ethanol-fuelled vehicles have been currently used on a large scale. This paper reports the atmospheric methanol and isoproponal concentrations which were measured from May to December 1997, in Osaka, Japan, where alcohol fuel was not used and from 3 to 9 February 1998 in Sao Paulo, Brazil, where ethanol was used. The alcohols were determined by the alkyl nitrite formation reaction using gas chromatography (GC-ECD) analysis. The concentration of atmospheric alcohols, especially ethanol, measured in Sao Paulo were significantly higher than those in Osaka. In Osaka, the average concentrations of atmospheric methanol, ethanol, and isopropanol were 5.8 ± 3.8, 8.2 ± 4.6, and 7.2 ± 5.9ppbv, respectively. The average ambient levels of methanol, ethanol, and isopropanol measured in Sao Paulo were 34.1± 9.2, 176.3 ± 38.1, and 44.2 ± 13.7ppbv, respectively. The ambient levels of aldehydes, which were expected to be high due to the use of alcohol fuel, were also measured at these sampling sites. The atmospheric formaldehyde average measured in Osaka was 1.9± 0.9ppbv, and the average acetaldehyde concentration was 1.5 ± 0.8ppbv. The atmospheric formaldehyde and acetaldehyde average concentrations measured in Sao Paulo were 5.0 ± 2.8 and 5.4 ± 2.8ppbv, respectively. The C 2 H 5 OH/CH 3 OH and CH 3 CHO/HCHO were compared between the two measurement sites and elsewhere in the world, which have already been reported in the literature. Due to the use of ethanol-fuelled vehicles, these ratios, especially C-2H 5 OH/CH 3 OH, are much higher in Brazil than those measured elsewhere in the world. (Author)

  5. Endovascular Sharp Recanalization for Calcified Femoropopliteal Artery Occlusion

    Directory of Open Access Journals (Sweden)

    Hsuan-Li Huang

    2012-01-01

    Full Text Available Endovascular intervention of peripheral chronic total occlusion (CTO is technically challenging and time consuming. Various techniques and devices are used to facilitate lesion crossing and improve the success rate of the procedure. However, these new devices are quite expensive and not readily available. We report 2 cases of peripheral CTO wherein the occlusions were successfully crossed by using stiff end of Terumo glidewire. This sharp recanalization may be a useful technique for the recanalization of calcified peripheral CTOs when conventional techniques fail and new devices are not readily available, but it is accompanied by the risk of distal atheroembolism.

  6. The efficacy of extracorporeal shock wave lithotripsy on single dense calcified gallstones according to computed tomography

    Energy Technology Data Exchange (ETDEWEB)

    Mori, Takao (Mie Univ., Tsu (Japan). School of Medicine); Shimono, Kazuko; Moriyama, Shigeru; Masuda, Touru; Ikeda, Tetsuya; Umegae, Satoru; Nagata, Norikazu

    1993-05-01

    The efficacy and complications of extracorporeal shock wave lithotripsy (ESWL) for single gallstones were compared between 15 patients with a CT-lucent stone and 18 patients with a dense calcified stone. In all of five patients with a stone smaller than 10 mm in diameter, complete or sufficient clearance was observed, regardless of calcification. However, in 28 patients with a stone larger than 11 mm in diameter, the rates of complete or sufficient clearance were lower in those with a dense calcified stone (64%) than in those with a computed tomography (CT) lucent stone (93%). There was no difference in the rate of complications between patients with a CT-lucent stone and those with a dense calcified stone. These results thus suggest that extracorporeal shock wave lithotripsy may be safely employed for patients with a single calcified gallstone. (author).

  7. Metal assessment in urban park soils in Sao Paulo 1. Ibirapuera Park

    International Nuclear Information System (INIS)

    Figueiredo, Ana Maria G.; Camargo, Sonia P.; Pavese, Arthur C.; Gumiero, Felipe C.; Enzweiler, Jacinta; Sigolo, Joel B.

    2007-01-01

    In the last years urban soils received increasing attention by scientists, leading to studies focused on their description and investigation all over the world, due to the increasing metal pollution derived from incinerators, industrial waste, atmospheric deposition of dust and aerosols, and other activities. Metal contamination in Sao Paulo public parks is an important environmental question and there is little information on this subject. As part of a project which aims metal assessment in urban park soils from Sao Paulo, in the present paper the concentration of the elements As, Ba, Cr, Pb, Sb and Zn were determined in surface soil samples (0-5 cm) from Ibirapuera park of Sao Paulo. Ibirapuera park is one of the biggest and most visited parks of the city of Sao Paulo, receiving during the weekends more than 400,000 visitors. Instrumental Neutron Activation Analysis (INAA) and X-ray Fluorescence (FRX) were used for metal analysis. Preliminary results showed concentration levels of the analyzed elements higher than the values considered as reference values for soils in Sao Paulo, according to the Environmental Protection Agency of the State of Sao Paulo (CETESB). For As, Ba, Cr and Sb, in some samples the concentrations were even higher than the Prevention values reported by CETESB. The high concentrations of the elements As, Ba, Cr, Pb, Sb and Zn in the Ibirapuera park top soils suggest an anthropogenic source and indicate a potential damage to soil quality. (author)

  8. In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation

    Science.gov (United States)

    Bloise, Nora; Saino, Enrica; Bragheri, Francesca; Minzioni, Paolo; Cristiani, Ilaria; Imbriani, Marcello; Visai, Livia

    2011-07-01

    The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm2, group III irradiated with a single dose of 3 J/cm2, and group IV and V exposed for three consecutive days to 1 or 3 J/cm², respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

  9. An exceptional collision tumor: gastric calcified stromal tumor and ...

    African Journals Online (AJOL)

    The authors report an exceptional case of collision tumor comprised of a gastric calcified stromal tumor and a pancreatic adenocarcinoma. The pancreatic tumor was detected fortuitously on the histological exam of resection specimen. Key words: Collision tumor, stromal tumor, adenocarcinoma ...

  10. Metal assessment in urban park soils in Sao Paulo 4. Alfredo Volpi (Morumbi Park)

    International Nuclear Information System (INIS)

    Camargo, Sonia P.; Figueiredo, Ana Maria G.; Pavese, Arthur C.; Gumiero, Felipe C.; Enzweiler, Jacinta

    2007-01-01

    The presence of elevated metal concentrations in soils of the urban environment has been recognized as an important source of metal intake to humans, particularly to children, which are more susceptible to the adverse effects of soil ingestion than adults. There has been little research on urban soils in Sao Paulo, a very populated city with severe pollution problems, and there is little information about metal concentration levels in public parks of Sao Paulo. As part of a project which aims metal assessment in urban park soils from Sao Paulo, in the present paper the concentration of the elements As, Ba, Cr, Sb and Zn were determined in topsoil samples (0- 5 cm and 0-20 cm) from Alfredo Volpi (Morumbi) park of Sao Paulo. Instrumental Neutron Activation Analysis (INAA) was used for metal analysis. Preliminary results showed higher concentrations of As, Ba and Sb compared with the values considered as reference for soils in Sao Paulo, according to Environmental Protection Agency of the State of Sao Paulo (CETESB). In some samples Ba showed concentration levels higher than the Prevention values reported by CETESB. (author)

  11. CT and MRI findings of calcified spinal meningiomas: correlation with pathological findings

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ji Won; Kim, Hak Jin [Pusan National University Hospital, Department of Radiology, Busan (Korea); Pusan National University School of Medicine, Medical Research Institute, Busan (Korea); Lee, In Sook [Pusan National University Hospital, Department of Radiology, Busan (Korea); Pusan National University School of Medicine, Medical Research Institute, Busan (Korea); Pusan National University School of Medicine, Department of Radiology, Busan (Korea); Choi, Kyung-Un [Pusan National University School of Medicine, Medical Research Institute, Busan (Korea); Pusan National University Hospital, Department of Pathology, Busan (Korea); Lee, Young Hwan [Catholic University of Daegu School of Medicine, Department of Radiology, Daegu (Korea); Yi, Jae Hyuck [Kyungpook National University Hospital, Department of Radiology, Daegu (Korea); Song, Jong Woon [Inje University Pusan Paik Hospital, Department of Radiology, Busan (Korea); Suh, Kyung Jin [Dongguk University Gyungju Hospital, College of Medicine, Dongguk University, Department of Radiology, Gyungju (Korea)

    2010-04-15

    This study was designed to present characteristic CT and MR findings of calcified spinal meningiomas that correlate with pathological findings and to assess the efficacy of CT for the detection of calcifications within a mass in comparison to MRI. Between 1998 and 2009, 10 out of 11 patients who had pathologically confirmed psammomatous meningiomas showed gross calcifications on CT images and were included in this study. On CT scans of the 10 patients, the distribution pattern, morphology and number of calcifications within masses were evaluated. MRI was performed in seven patients and signal intensities of masses were assessed. The pathological results analyzed semi-quantitatively were compared with the density or the size of calcifications within a mass as seen on a CT scan. Seven of 10 masses were located at the thoracic spine level. Eight masses had intradural locations. The other two masses had extradural locations. Four masses were completely calcified based on standard radiographs and CT. Symptoms duration, the size of the mass and size or number of calcifications within a mass had no correlation. The location, size, and distribution pattern of calcifications within masses were variable. On MR images, signal intensity of calcified tumor varied on all imaging sequences. All the masses enhanced after injection of intravenous contrast material. A calcified meningioma should be first suggested when extradural or intradural masses located in the spine contain calcifications regardless of the size or pattern as depicted on CT, especially in the presence of enhancement as seen on MR images. (orig.)

  12. A case of multiple cardiac calcified amorphous tumours

    Directory of Open Access Journals (Sweden)

    Amrit Chowdhary

    2017-05-01

    Full Text Available Cardiac calcified amorphous tumours of the heart are rare non-neoplastic cardiac masses that can present like a malignant mass or an intra-cardiac thrombus. We report an extremely unusual case of a 73 year old man who presented to hospital with dyspnoea and subsequent investigations revealed multiple cardiac CATs.

  13. Technetium-99m-HEDP concentration in calcified myoma

    International Nuclear Information System (INIS)

    Ell, P.J.; Breitfellner, G.; Meixner, M.

    1976-01-01

    This case emphasizes once more the need to interpret data in the clinical context, and it describes for the first time a concentration of /sup 99m/Tc-labeled HEDP in a calcified myoma of the uterus. Soft-tissue concentration of labeled phosphates should always be kept in mind when interpreting whole-body bone scans

  14. Nodular calcified neurocysticercosis with signs of reactivation

    Energy Technology Data Exchange (ETDEWEB)

    Coeli, Gustavo Nunes Medina; Tiengo, Rodrigo Ribeiro; Silva, Guilherme Carlos da; Silva, Leandro Urquiza Marques Alves da, E-mail: gustavonmc@yahoo.com.br [Department of Radiology and Imaging Diagnosis, Hospital Escola de Itajuba, MG (Brazil); Silva, Afonso Carlos da [Medical Practice, Hospital Escola de Itajuba, MG (Brazil); Fernandes, Jose Otavio Meyer [Clinica Sul Mineira Tomosul and Clinica Magsul, Itajuba, MG (Brazil)

    2012-09-15

    Neurocysticercosis is a disease characterized by the involvement of the central nervous system by the intermediate larval stage of the parasite Taenia solium. The larva degeneration process and the inflammatory reaction of the body cause clinical symptoms. The authors report a case of clinical and radiological reactivation of nodular calcified neurocysticercosis in a patient who was asymptomatic for more than 20 years. Antiparasitic treatment showed a good response (author)

  15. Community-level sensitivity of a calcifying ecosystem to acute in situ CO2 enrichment

    KAUST Repository

    Burdett, HL

    2017-11-23

    The rate of change in ocean carbonate chemistry is a vital determinant in the magnitude of effects observed. Benthic marine ecosystems are facing an increasing risk of acute CO2 exposure that may be natural or anthropogenically derived (e.g. engineering and industrial activities). However, our understanding of how acute CO2 events impact marine life is restricted to individual organisms, with little understanding for how this manifests at the community level. Here, we investigated in situ the effect of acute CO2 enrichment on the coralline algal ecosystem—a globally ubiquitous, ecologically and economically important habitat, but one which is likely to be sensitive to CO2 enrichment due to its highly calcified reef-like structures engineered by coralline algae. Most notably, we observed a rapid community-level shift to favour net dissolution rather than net calcification. Smaller changes from net respiration to net photosynthesis were also observed. There was no effect on the net flux of DMS/DMSP (algal secondary metabolites), nor on the nutrients nitrate and phosphate. Following return to ambient CO2 levels, only a partial recovery was seen within the monitoring timeframe. This study highlights the sensitivity of biogenic carbonate marine communities to acute CO2 enrichment and raises concerns over the capacity for the system to ‘bounce back’ if subjected to repeated acute high-CO2 events.

  16. Problems of small hydroelectric power plants - SHP: the example of Sao Paulo State, Brazil; Problematica de desenvolvimento das pequenas centrais hidreletricas-PCH: o exemplo do Estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Matajs, R.R. [Sao Paulo Univ., SP (Brazil). Programa Interunidades de Pos-Graduacao em Energia; Fabrizy, M.P. [Sao Paulo Univ., SP (Brazil). Inst. de Eletrotecnica e Energia

    1994-07-01

    This paper presents a survey of the small hydroelectric potential in the State of Sao Paulo - Brazil. Three projections will be presented as as attempt to show the possible participation of the SHP in a sort, medium and long term, in the State of Sao Paulo energetic matrix.

  17. Orbital atherectomy for the treatment of severely calcified coronary lesions: evidence, technique, and best practices.

    Science.gov (United States)

    Shlofmitz, Evan; Martinsen, Brad J; Lee, Michael; Rao, Sunil V; Généreux, Philippe; Higgins, Joe; Chambers, Jeffrey W; Kirtane, Ajay J; Brilakis, Emmanouil S; Kandzari, David E; Sharma, Samin K; Shlofmitz, Richard

    2017-11-01

    The presence of severe coronary artery calcification is associated with higher rates of angiographic complications during percutaneous coronary intervention (PCI), as well as higher major adverse cardiac events compared with non-calcified lesions. Incorporating orbital atherectomy (OAS) for effective preparation of severely calcified lesions can help maximize the benefits of PCI by attaining maximal luminal gain (or stent expansion) and improve long-term outcomes (by reducing need for revascularization). Areas covered: In this manuscript, the prevalence, risk factors, and impact of coronary artery calcification on PCI are reviewed. Based on current data and experience, the authors review orbital atherectomy technique and best practices to optimize lesion preparation. Expert Commentary: The coronary OAS is the only device approved for use in the U.S. as a treatment for de novo, severely calcified coronary lesions to facilitate stent delivery. Advantages of the device include its ease of use and a mechanism of action that treats bi-directionally, allowing for continuous blood flow during treatment, minimizing heat damage, slow flow, and subsequent need for revascularization. The OAS technique tips reviewed in this article will help inform interventional cardiologists treating patients with severely calcified lesions.

  18. In Vivo Remineralization of Artificial Carious Lesions using Calcifying Solution and Fluoride Solution

    Directory of Open Access Journals (Sweden)

    Els Sunarsih Budipramana

    2015-10-01

    Full Text Available The remineralization potential of fluoride and calcifying solution was studiedas in situ model. Matched enamel discs of artifically demineralized human enamel were attached to an acrylic mandibular removable appliance of 6 adult volunteers who rinsed their mouth with a solution containing either 50 ppm F-, 200 ppm F- in amine fluoride, calcifying solution (formula BR21 or placebo as a control. The volunteers were asked to rinse 3 times a day for 3 minutes with 15 ml of the solution for 6 days (18 times. On the 7th day enamel discs were taken out from the partial denture. Three kinds of measurements were done: enamel permeability testing, depth lesion testing and fluoride retention testing. New demineralized enamel discs were attached to the partial dentures and the volunteers were asked to start rinsing with other solutions using the same protocols. The purpose of this study was to answer the question why the calcifying solutions were no more used as a remineralizing solution. The data ws analyzed using SPSS/PC for two factors Anova and one way Anova for enamel permeability and Kruskal Wallis for studying enamel depth lesion and fluoride retention. There was no significant difference after rinsing with calcifying solution and placebo in enamel permeability, depth lesions, and fluoride retention when compared to fluoride solution. To get a better result in remineralizing carious teeth fluoride contents in solution must be higher than 50 ppm F-.

  19. LIBS analysis of artificial calcified tissues matrices.

    Science.gov (United States)

    Kasem, M A; Gonzalez, J J; Russo, R E; Harith, M A

    2013-04-15

    In most laser-based analytical methods, the reproducibility of quantitative measurements strongly depends on maintaining uniform and stable experimental conditions. For LIBS analysis this means that for accurate estimation of elemental concentration, using the calibration curves obtained from reference samples, the plasma parameters have to be kept as constant as possible. In addition, calcified tissues such as bone are normally less "tough" in their texture than many samples, especially metals. Thus, the ablation process could change the sample morphological features rapidly, and result in poor reproducibility statistics. In the present work, three artificial reference sample sets have been fabricated. These samples represent three different calcium based matrices, CaCO3 matrix, bone ash matrix and Ca hydroxyapatite matrix. A comparative study of UV (266 nm) and IR (1064 nm) LIBS for these three sets of samples has been performed under similar experimental conditions for the two systems (laser energy, spot size, repetition rate, irradiance, etc.) to examine the wavelength effect. The analytical results demonstrated that UV-LIBS has improved reproducibility, precision, stable plasma conditions, better linear fitting, and the reduction of matrix effects. Bone ash could be used as a suitable standard reference material for calcified tissue calibration using LIBS with a 266 nm excitation wavelength. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Treatment of Necrotic Calcified Tooth Using Intentional Replantation Procedure

    Directory of Open Access Journals (Sweden)

    Nima Moradi Majd

    2014-01-01

    Full Text Available Introduction. If the teeth are impacted by a chronic irritant, the pulp space possibly will undergo calcific changes that may impede access opening during root canal treatment. In such cases that conventional endodontic treatment is impossible or impractical, intentional replantation may be considered as a last solution to preserve the tooth. Methods. After failing to perform conventional root canal therapy for a necrotic calcified right mandibular second premolar, the tooth was gently extracted. The root apex was resected and the root end cavity was prepared and filled with calcium enriched mixture (CEM cement. Then, the extracted tooth was replanted in its original position. Results. After a year the tooth was asymptomatic, and the size of periapical radiolucency was remarkably reduced and no clinical sign of ankylosis was observed. Conclusion. Intentional replantation of the necrotic calcified teeth could be considered as an alternative to teeth extraction, especially for the single-rooted teeth and when nonsurgical and surgical endodontic procedures seem impossible.

  1. Epidural tumour (calcified fibroma) as cause of a 'Cervical Syndrome'

    Energy Technology Data Exchange (ETDEWEB)

    Piotrowski, W P

    1983-01-01

    A calcified fibroma caused a so called Cervical Syndrome not responding to medical treatment. In the computerized tomography a compression of the cervical spinal cord could be demonstrated. From this the indication for the operation was given.

  2. Epidural tumour (calcified fibroma) as cause of a 'Cervical Syndrome'

    International Nuclear Information System (INIS)

    Piotrowski, W.P.

    1983-01-01

    A calcified fibroma caused a so called cervical syndrome not responding to medical treatment. In the computerized tomography a compression of the cervical spinal cord could be demonstrated. From this the indication for the operation was given. (Author)

  3. Use of Aromatase Inhibitors in Large Cell Calcifying Sertoli Cell Tumors: Effects on Gynecomastia, Growth Velocity, and Bone Age

    Science.gov (United States)

    Crocker, Melissa K.; Gourgari, Evgenia; Stratakis, Constantine A.

    2014-01-01

    Context: Large cell calcifying Sertoli cell tumors (LCCSCT) present in isolation or, especially in children, in association with Carney Complex (CNC) or Peutz-Jeghers Syndrome (PJS). These tumors overexpress aromatase (CYP19A1), which leads to increased conversion of delta-4-androstenedione to estrone and testosterone to estradiol. Prepubertal boys may present with growth acceleration, advanced bone age, and gynecomastia. Objective: To investigate the outcomes of aromatase inhibitor therapy (AIT) in prepubertal boys with LCCSCTs. Design: Case series of a very rare tumor and chart review of cases treated at other institutions. Setting: Tertiary care and referral center. Patients: Six boys, five with PJS and one with CNC, were referred to the National Institutes of Health for treatment of LCCSCT. All patients had gynecomastia, testicular enlargement, and advanced bone ages, and were being treated by their referring physicians with AIT. Interventions: Patients were treated for a total of 6–60 months on AIT. Main Outcome Measures: Height, breast tissue mass, and testicular size were all followed; physical examination, scrotal ultrasounds, and bone ages were obtained, and hormonal concentrations and tumor markers were measured. Results: Tumor markers were negative. All patients had decreases in breast tissue while on therapy. Height percentiles declined, and predicted adult height moved closer to midparental height as bone age advancement slowed. Testicular enlargement stabilized until entry into central puberty. Only one patient required unilateral orchiectomy. Conclusions: Patients with LCCSCT benefit from AIT with reduction and/or elimination of gynecomastia and slowing of linear growth and bone age advancement. Further study of long-term outcomes and safety monitoring are needed but these preliminary data suggest that mammoplasty and/or orchiectomy may be foregone in light of the availability of medical therapy. PMID:25226294

  4. Receptors and effects of gut hormones in three osteoblastic cell lines

    Directory of Open Access Journals (Sweden)

    Wilson Peter JM

    2011-07-01

    Full Text Available Abstract Background In recent years the interest on the relationship of gut hormones to bone processes has increased and represents one of the most interesting aspects in skeletal research. The proportion of bone mass to soft tissue is a relationship that seems to be controlled by delicate and subtle regulations that imply "cross-talks" between the nutrient intake and tissues like fat. Thus, recognition of the mechanisms that integrate a gastrointestinal-fat-bone axis and its application to several aspects of human health is vital for improving treatments related to bone diseases. This work analysed the effects of gut hormones in cell cultures of three osteoblastic cell lines which represent different stages in osteoblastic development. Also, this is the first time that there is a report on the direct effects of glucagon-like peptide 2, and obestatin on osteoblast-like cells. Methods mRNA expression levels of five gut hormone receptors (glucose-dependent insulinotropic peptide [GIP], glucagon-like peptide 1 [GLP-1], glucagon-like peptide 2 [GLP-2], ghrelin [GHR] and obestatin [OB] were analysed in three osteoblastic cell lines (Saos-2, TE-85 and MG-63 showing different stages of osteoblast development using reverse transcription and real time polymerase chain reaction. The responses to the gut peptides were studied using assays for cell viability, and biochemical bone markers: alkaline phosphatase (ALP, procollagen type 1 amino-terminal propeptides (P1NP, and osteocalcin production. Results The gut hormone receptor mRNA displayed the highest levels for GIP in Saos-2 and the lowest levels in MG-63, whereas GHR and GPR39 (the putative obestatin receptor expression was higher in TE-85 and MG-63 and lower in Saos-2. GLP-1 and GLP-2 were expressed only in MG-63 and TE-85. Treatment of gut hormones to cell lines showed differential responses: higher levels in cell viability in Saos-2 after GIP, in TE-85 and MG-63 after GLP-1, GLP-2, ghrelin and

  5. A pigmented calcifying cystic odontogenic tumor associated with compound odontoma: a case report and review of literature

    Directory of Open Access Journals (Sweden)

    Borkosky Silvia S

    2007-09-01

    Full Text Available Abstract Background Pigmented intraosseous odontogenic lesions are rare with only 47 reported cases in the English literature. Among them, pigmented calcifying cystic odontogenic tumor, formerly known as calcifying odontogenic cyst, is the most common lesion with 20 reported cases. Methods A case of pigmented calcifying cystic odontogenic tumor associated with odontoma occurring at the mandibular canine-premolar region of a young Japanese boy is presented with radiographic, and histological findings. Special staining, electron microscopic study and immunohistochemical staining were also done to characterize the pigmentation. Results The pigments in the lesion were confirmed to be melanin by Masson-Fontana staining and by transmission electron microscopy. The presence of dendritic melanocytes within the lesion was also demonstrated by S-100 immunostaining. Conclusion The present case report of pigmented calcifying cystic odontogenic tumor associated with odontoma features a comprehensive study on melanin and melanocytes, including histochemical, immunohistochemical and transmission electron microscopic findings.

  6. Case report - calcification of the medial collateral ligament of the knee with simultaneous calcifying tendinitis of the rotator cuff

    OpenAIRE

    Kamawal, Yama; Steinert, Andre F; Holzapfel, Boris M; Rudert, Maximilian; Barthel, Thomas

    2017-01-01

    Background Calcification of the medial collateral ligament (MCL) of the knee is a very rare disease. We report on a case of a patient with a calcifying lesion within the MCL and simultaneous calcifying tendinitis of the rotator cuff in both shoulders. Case presentation Calcification of the MCL was diagnosed both via x-ray and magnetic resonance imaging (MRI) and was successfully treated surgically. Calcifying tendinitis of the rotator cuff was successfully treated applying conservative method...

  7. Atherectomy for calcified coronary lesions: When and how?

    Science.gov (United States)

    Karatasakis, Aris; Brilakis, Emmanouil S

    2016-03-01

    Percutaneous coronary intervention of heavily calcified lesions can be challenging. Although the ROTAXUS trial did not demonstrate long-term clinical benefit with routine rotational atherectomy, atherectomy remains an indispensable tool to achieve acute procedural success. Until new data becomes available determining when and how to optimally use coronary atherectomy depends heavily on personal experience and clinical judgment. © 2016 Wiley Periodicals, Inc.

  8. Response of the calcifying coccolithophore Emiliania huxleyi to low pH/high pCO2: from physiology to molecular level

    NARCIS (Netherlands)

    Richier, S.; Fiorini, S.; Kerros, M.E.; von Dassow, P.; Gattuso, J.P.

    2011-01-01

    The emergence of ocean acidification as a significant threat to calcifying organisms in marine ecosystems creates a pressing need to understand the physiological and molecular mechanisms by which calcification is affected by environmental parameters. We report here, for the first time, changes in

  9. Optimization of the daily operation of the plants of Medio Sao Francisco; Otimizacao da operacao diaria das usinas do Medio Sao Francisco

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, Jessica Pillon Torralba; Colnago, Glauber Renato; Correia, Paulo de Barros; Ohishi, Takaaki [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica. Dept. de Energia], emails: pillon@fem.unicamp.br, colnago@fem.unicamp.br, pcorreia@fem.unicamp.br, taka@densis.fee.unicamp.br

    2010-07-01

    This paper presents an optimization model for daily operation of Sao Francisco hydroelectric power plants. The study considers eight power plants - Sobradinho (USB), Luiz Gonzaga (ULG), Apolonio Sales (UAS), Paulo Afonso I, II, III, (UPA), Paulo Afonso IV (USQ) e Xingo (UXG)- belongs to Sao Francisco Hydroelectric Company (CHESF). Its objective is to maximize the power plant efficiency and to minimize the number of startup and shutdowns of generating units (GU), simultaneously. Considering those GU are equal, is determined the number of units to be dispatched and their charge. The optimal dispatch, linear and non-linear programming techniques and genetic algorithms (GA) support this article. (author)

  10. Thyroid Cancer Incidence Patterns in Sao Paulo, Brazil, and the U.S. SEER Program, 1997–2008

    Science.gov (United States)

    Neta, Gila; Aschebrook-Kilfoy, Briseis; Ron, Elaine; Devesa, Susan S.

    2013-01-01

    Background Thyroid cancer incidence has risen steadily over the last few decades in most of the developed world, but information on incidence trends in developing countries is limited. Sao Paulo, Brazil, has one of the highest rates of thyroid cancer worldwide, higher than in the United States. We examined thyroid cancer incidence patterns using data from the Sao Paulo Cancer Registry (SPCR) in Brazil and the National Cancer Institute's Surveillance Epidemiology End Results (SEER) program in the United States. Methods Data on thyroid cancer cases diagnosed during 1997–2008 were obtained from SPCR (n=15,892) and SEER (n=42,717). Age-adjusted and age-specific rates were calculated by sex and histology and temporal patterns were compared between the two populations. Results Overall incidence rates increased over time in both populations and were higher in Sao Paulo than in the United States among females (SPCR/SEER incidence rate ratio [IRR]=1.65) and males (IRR=1.23). Papillary was the most common histology in both populations, followed by follicular and medullary carcinomas. Incidence rates by histology were consistently higher in Sao Paulo than in the United States, with the greatest differences for follicular (IRR=2.44) and medullary (IRR=3.29) carcinomas among females. The overall female/male IRR was higher in Sao Paulo (IRR=4.17) than in SEER (IRR=3.10) and did not change over time. Papillary rates rose over time more rapidly in Sao Paulo (annual percentage change=10.3% among females and 9.6% among males) than in the United States (6.9% and 5.7%, respectively). Regardless of sex, rates rose faster among younger people (8 among both Sao Paulo males and females, in contrast to increases from 9 to 12 and from 6 to 7 among U.S.males and females, respectively. Conclusions Increased diagnostic activity may be contributing to the notable rise in incidence, mainly for papillary type, in both populations, but it is not likely to be the only reason. Differences in

  11. Co-occurrence of Calcifying Odontogenic Cyst, Aggressive Central Giant Cell Granuloma and Central Odontogenic Fibroma: Report of a Very Rare Entity and Its Surgical Management

    Directory of Open Access Journals (Sweden)

    Touraj Vaezi

    2016-09-01

    Full Text Available Calcifying odontogenic cyst (COC, Central odontogenic fibroma (COF and aggressive central giant cell granuloma (CGCG are rare pathologic diseases affecting the jaws. While the Co-existence of two of them is reported in the literature, existence of all three conditions in one patient is an extremely rare entity. In the present report, initial biopsy revealed fibrosarcoma, therefore mandibular resection was performed for the subject. Sectional Histopathologic evaluation revealed the co-existence of three conditions through histopathologic evaluation. This report emphasizes the importance of precise microscopical evaluation of jaw lesions and thorough sectional examination of the lesions to reach the precise diagnosis. Treatment modalities and follow-up radiographs are also provided to help clinicians manage these entities.

  12. Calcifying Odontogenic Cyst with Complex Odontoma: Histological and Immunohistochemical Features

    Directory of Open Access Journals (Sweden)

    Nooshin Mohtasham

    2013-01-01

    Full Text Available The calcifying odontogenic cyst (COC is a rare odontogenic cyst. Only 2% of all odontogenic cysts and tumors are COC. COC associated with odontoma (COCaO reported in 24% of COCs. COCaO presents a greater incidence in female, with a ratio of 2 to 1. The highest incidence of COCaO occurs during the second decade with a mean age of 16 years, most frequently occurring in the maxilla (61.5%. Here, we describe a classic case of COCaO of the maxillary incisor-canine region in 17-year-old girl, and discuss the clinicopathological features and immunohistochemical finding of this tumor.

  13. Calcifying Odontogenic Cyst with Complex Odontoma: Histological and Immunohistochemical Features

    Directory of Open Access Journals (Sweden)

    Mohsen Merati

    2012-09-01

    Full Text Available The calcifying odontogenic cyst (COC is a rare odontogenic cyst. Only 2% of all odontogenic cysts and tumors are COC. COC associated with odontoma (COCaO reported in 24% of COCs. COCaO presents a greater incidence in female, with a ratio of 2 to 1. The highest incidence of COCaO occurs during the second decade with a mean age of 16 years, most frequently occurring in the maxilla (61.5%. Here, we describe a classic case of COCaO of the maxillary incisor-canine region in 17-year-old girl, and discuss the clinicopathological features and immunohistochemical finding of this tumor.

  14. Predictive Factors of Potential Malignant Transformation in Recurrent Calcifying Cystic Odontogenic Tumor: Review of the Literature

    Directory of Open Access Journals (Sweden)

    Sepideh Mokhtari

    2013-01-01

    Full Text Available Calcifying cystic odontogenic tumor (CCOT demonstrates considerable diversity in histopathology and clinical behavior. Ghost cell odontogenic carcinoma (GCOC is the rare malignant counterpart of CCOT and it frequently arises from malignant transformation of a recurrent CCOT. In this paper, we present a case of CCOT and discuss its distinct histopathologic features in recurrence. Then, we will have a review on clinical, histopathological, and immunohistochemical aspects of GCOC in the literature. Predictive factors of malignant transformation in a benign CCOT will also be discussed.

  15. The problem of calcified ascending aorta during aortocoronary bypass

    Directory of Open Access Journals (Sweden)

    Nežić Duško G.

    2004-01-01

    Full Text Available Heavily calcified ascending aorta significantly increased morbidity and lethality during open-heart surgery. Cannulation and clamping (partial or total of severely atherosclerotic ascending aorta can easily cause damage and rupture of aortic wall, with consequential distal (often fatal embolization with atheromatous debris (brain, myocardium. From June 1998. until June 2000, 11 of 2 136 (0.5% patients who underwent coronary artery bypass grafting were with the severe atheromatous ascending aorta. The site of cannulation was in the aortic arch in three patients (aorta was occluded with Foley catheter in one case, and single clamp technique was used in the other two cases. The femoral artery was the cannulation site in other five cases. Profound hypothermia, ventricular fibrillation, and circulatory arrest, with no cross-clamping or cardioplegia, were used in three patients. Two patients were operated on with extracorporeal circulation, one in normothermia, on the beating heart, the other in moderate hypothermia, on fibrillating heart. In three patients myocardial revascularization was performed on the beating heart, in normothermia, without extracorporeal circulation. Postoperative course was uneventful in all 11 patients. Neither atheroembolism in the peripheral organs, nor atheroembolism of the extramities occurred. The proposed surgical approaches have the potential to reduce the prevalence of stroke and systemic embolization associated with coronary artery bypass grafting in patients with heavily calcified ascending aorta. This result was achieved due to the applied modifications of standard cardiosurgical technique.

  16. Energy balance of the state of Sao Paulo - 2015: calendar year 2014; Balanco energetico do estado de Sao Paulo - 2015: ano base 2014

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-07-01

    The State Energy Report 2015 prepared by the Secretariat of Energy, is the publication of the State Government that shows the supply and demand of energy used by the state economy, with the corresponding participation of the sectors involved. The energy policy of the State of Sao Paulo's strategic objective of expanding the energy supply from the load centers, energy security and expanding the share of renewable sources in the matrix. In order to achieve these objectives it is essential that count on complete and reliable information on the supply and demand of energy. This information is needed not only to the Secretary of Energy, in the formulation and implementation of state programs, but the municipalities and regions of the state so that they can formulate and present their demands as clearly as possible. It is therefore the production of strategic information to the counties together with the State Government to plan its priorities in energy and services. The publication is essential reading for all who work with energy planning studies, feasibility of innovative technologies, search for energy efficiency and environmental protection. The evolution of the energy matrix of the State of Sao Paulo is one of the Balance sheet highlights, showing a greater share of biomass and natural gas since the mid- 90s . This issue also reports on CO{sub 2} emissions in the state, historical data vintages of the Sao Paulo sugarcane sector and a breakdown of electricity and derived from oil and gas. Thus, the State Energy Balance 2015 becomes an important tool aimed at implementation of public policies relevant to the infrastructure sector. (author)

  17. 137Cs inventory in sedimentary columns from continental shelf of Sao Paulo state

    International Nuclear Information System (INIS)

    Figueira, Rubens C.L.; Cordero, Luisa M.; Mahiques, Michel M. de; Tessler, Moyses G.

    2009-01-01

    137 Cs is an artificial radioactive isotope produced by 235 U fission. This radionuclide has a high fission yield and a half-life of 30 years. It has been detected in the environment since 1945 and its principal contamination source has been nuclear tests in the atmosphere. There are other sources of 137 Cs contamination in the environment, such as: release from nuclear and reprocessing plants, radioactive dumping and nuclear accidents (Chernobyl, for example). This paper presents an inventory of 137 Cs on the Continental Shelf of Sao Paulo State, a region located between Cabo de Santa Marta Grande (Santa Catarina state) and Cabo Frio (Rio de Janeiro state). In this area, 9 cores were collected by the Instituto Oceanografico da Universidade de Sao Paulo (Sao Paulo University Institute of Oceanography). The cores were sliced at every 2 cm; sub-samples were lyophilized, grinded and stored in plastic containers. 137 Cs was determined by 661 keV photopeak using a gamma spectrometry detector (Ge hyperpure). The analysis was performed by efficiency and background in different counting times. 137 Cs concentration activities varied from 0.3 to 3.6 Bq kg -1 with a mean value of 1.2±0.6 Bq kg -1 . The inventory of 137 Cs in this area was 13±7 Bq m-2. Values obtained are in agreement with the Southern Hemisphere, a region contaminated by atmospheric fallout due to past nuclear explosions. (author)

  18. Uranium occurrence at Sao Teodosio farm, Currais Novos, Rio Grande do Norte, Brazil

    International Nuclear Information System (INIS)

    Favali, J.C.; Leal, J.R.L.V.

    1974-01-01

    The areas of certain radiometric anomalies discovered in Serido geosyncline were selected for intensive study because of the similarity of the geology to that of known uranium deposits in other parts of the world. The uranium mineralization at Sao Teodosio farm, near Currais Novos, RN, on the Serrinha anticline axis, occurs in alaskite similar to that at Rossing in Southwest Africa. The Rossing deposit is the best example of the model proposed by Armstrong (1974) as 'porphyry' uranium deposits. That uranium deposit presents low grade of uranium content, about 0,030% U 3 O 8 and hundred thousands tons of uranium oxide. At Sao Teodosio occurs 550 m.y. alaskitic pegmatitic granite and garnet-quartz-biotite-schists of Serido formation, Upper Precambrian. These older rocks are cut by diabases dykes of Upper Terciary age. Uranium mineralization is associated with pegmatitic granite bodies similar to dykes and sills. The most common uranium minerals are: uraninite, meta-autunite and uranophane. Oligoclase, microline and quartz are the most frequent minerals. Acessory minerals are magnetite, titanite and zircon. Uranium oxide content at Sao Teodosio is 0,023% and average thickness is 2,80 m [pt

  19. SEGMENTATION IN TOURISTIC SECTOR: LGBT TOURISTS OF SAO PAULO

    Directory of Open Access Journals (Sweden)

    Maurício Sanitá Azevedo

    2012-05-01

    Full Text Available The objective of this article is to present the tourism segment LGBT (Lesbian, Gays, Bisexual and Transgender of Sao Paulo city and gather information about his profile as important support for the tourism sector in defining their marketing strategies. It was carried out an exploratory and statistical data from government and representatives of this public for a description and analysis of data on the composition and profile of this segment, as well as the strategies being used by companies to captivate it and loyalty it. It also composes a Descriptive Study carried out by the Tourism Observatory of São Paulo next to the participants of the Sao Paulo Gay Parade in 2011. As results, it appears that LGBT public presents behavioral particularities as buyers of tourist products, providing during their stay, higher investments than others tourism segments, because it comes as a tourist for a longer period of stay in Sao Paulo, thus leaving more financial resources in the city. It also presents as results, examples of effective use of differentiation strategies by organizations, attraction, service and loyalty in this segment.

  20. [Radial shock wave therapy in calcifying tendinitis of the rotator cuff--a prospective study].

    Science.gov (United States)

    Magosch, P; Lichtenberg, S; Habermeyer, P

    2003-01-01

    The aim of the study is to evaluate the influence of radial shock wave therapy (RSWT) on the course of calcifying tendinitis of the rotator cuff. 35 patients with a mean age of 47.5 years suffering from calcifying tendinitis stage Gaertner 2 with a mean size of 16.6 mm in typical location (true-ap view) for a mean of 28 months were treated by low-energy RSWT three times. The acromio-humeral distance averaged 10.4 mm measured at the true-ap view. All patients were clinically and radiologically followed-up at 4 weeks, 3, 6 and 12 months after the last treatment. The Constant score improved significantly (p energy RSWT leads within the first 4 weeks to a significant pain relief and an improvement of shoulder function. In consideration of the long history, the size and the spontaneous resorption rate of the calcific deposit, an inductive effect of RSWT on the resorption of the calcific deposit can be assumed.

  1. Surgical Management of Calcified Liver Hydatid Cyst Complicated with Thoracobiliary Fistula: A Case Series and Literature Review

    Directory of Open Access Journals (Sweden)

    Mohsen Sokouti

    2016-06-01

    Full Text Available Thoracobiliary fistula is a rare complication of hydatid cyst of the liver especially in the calcified form. Surgery is the only medical option. The treatment consists of radical surgical procedures in the majority of the patients. Conservative surgical treatments are performed with high mortality rate. Herein, we will describe two patients of calcified hydatid cysts of the liver whose condition becomes complicated with Thoracobiliary fistula. The first patient was treated with right thoracotomy and resection of pleural hydatid cysts. Then, were evacuated the ruptured laminated membrane and daughter cysts of infected hepatic hydatid cysts through diaphragmatic opening and sub diaphragmatic drainage of the calcified liver hydatid cyst. The second patient was also treated with right thoracotomy, resection of pulmonary hydatid cysts, evacuation of ruptured bile stained laminated membrane and daughter cysts of hepatic hydatid cysts through diaphragmatic opening and sub diaphragmatic drainage of the calcified cyst cavity. Our patients underwent conservative surgery which posed a severe risk. Both cases are discussed together with review of the literature.

  2. The expansion of sugar cane cultivation in the Sao Paulo state, Brazil; A expansao da cultura canavieira no estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Suani Teixeira [Centro Nacional de Referencia em Biomassa (CENBIO), Sao Paulo, SP (Brazil); Lora, Beatriz Acquaro; Monteiro, Maria Beatriz C.A.

    2008-07-01

    The present work aimed at analyze the sugar cane culture expansion in substitution to the other cultures in the Sao Paulo state from available georeferred and statistical data. The results had evidenced clear expansion of the sugar cane culture in the state, having totalized a growth of 26.04% in the period from 2003 to 2006, with expressive increase in the regions of President Prudente, Sao Jose do Rio Preto, Barretos and Marilia that had, each one, more than 40% of increase in the period. Concomitantly it had reduction of the maize areas in the studied region and pasture areas stability, followed of a small increase of the cattle heads number. (author)

  3. Calcifying supracoracoid bursitis as a cause of chronic shoulder pain.

    Science.gov (United States)

    Mens, J; van der Korst, J K

    1984-01-01

    A case of chronic shoulder pain is reported with marked limitation of both active and passive elevations and a normal range of motion of the glenohumeral joint. X-ray examination demonstrated cloudy calcification in the coracoclavicular region, presumably indicating calcifying supracoracoid bursitis. Images PMID:6497468

  4. Orbital atherectomy for severely calcified lesions: More dissections in women but similar 30-day outcomes to men.

    Science.gov (United States)

    Chandrasekhar, Jaya; Mehran, Roxana

    2016-03-01

    Calcified lesions are associated with lower rates of successful percutaneous coronary intervention (PCI), greater stent thrombosis, and increased target vessel revascularization. Women undergoing PCI are more often older than men and likely to present with severe lesion calcification. The ORBIT II study, for the first time compares the effect of the orbital atherectomy system (OAS) in men and women undergoing PCI for severely calcified lesions. Although the adjusted risk of severe dissections was higher in women, the incidence of in-hospital and 30-day outcomes was similar to men. Randomized comparisons of the OAS with rotational atherectomy and with stenting without atherectomy are needed to further elucidate sex-based differences in calcified lesion PCI. © 2016 Wiley Periodicals, Inc.

  5. Different Expression and Localization of Phosphoinositide Specific Phospholipases C in Human Osteoblasts, Osteosarcoma Cell Lines, Ewing Sarcoma and Synovial Sarcoma

    Directory of Open Access Journals (Sweden)

    V.Vasco

    2017-06-01

    Full Text Available Background: Bone hardness and strength depends on mineralization, which involves a complex process in which calcium phosphate, produced by bone-forming cells, was shed around the fibrous matrix. This process is strictly regulated, and a number of signal transduction systems were interested in calcium metabolism, such as the phosphoinositide (PI pathway and related phospholipase C (PLC enzymes. Objectives: Our aim was to search for common patterns of expression in osteoblasts, as well as in ES and SS. Methods: We analysed the PLC enzymes in human osteoblasts and osteosarcoma cell lines MG-63 and SaOS-2. We compared the obtained results to the expression of PLCs in samples of patients affected with Ewing sarcoma (ES and synovial sarcoma (SS. Results: In osteoblasts, MG-63 cells and SaOS-2 significant differences were identified in the expression of PLC δ4 and PLC η subfamily isoforms. Differences were also identified regarding the expression of PLCs in ES and SS. Most ES and SS did not express PLCB1, which was expressed in most osteoblasts, MG-63 and SaOS-2 cells. Conversely, PLCB2, unexpressed in the cell lines, was expressed in some ES and SS. However, PLCH1 was expressed in SaOS-2 and inconstantly expressed in osteoblasts, while it was expressed in ES and unexpressed in SS. The most relevant difference observed in ES compared to SS regarded PLC ε and PLC η isoforms. Conclusion: MG-63 and SaOS-2 osteosarcoma cell lines might represent an inappropriate experimental model for studies about the analysis of signal transduction in osteoblasts

  6. Consequências cardiovasculares na SAOS Cardiovascular consequences of obstructive sleep apnea syndrome

    Directory of Open Access Journals (Sweden)

    Geraldo Lorenzi Filho

    2010-06-01

    Full Text Available Uma condição clínica muito comum é SAOS, que está associada a várias doenças cardiovasculares, incluindo hipertensão arterial sistêmica, fibrilação atrial e aterosclerose. A associação entre SAOS e doença cardiovascular não é somente uma consequência da sobreposição de fatores de risco, incluindo obesidade, sedentarismo, ser do sexo masculino e ter idade maior. Existem evidências crescentes de que SAOS contribui de forma independente para o aparecimento e a progressão de várias doenças cardiovasculares. Os mecanismos pelos quais SAOS pode afetar o sistema cardiovascular são múltiplos e incluem a ativação do sistema nervoso simpático, inflamação sistêmica, resistência a insulina e geração de estresse oxidativo. Existem evidências que o tratamento de SAOS com CPAP pode reduzir a pressão arterial, sinais precoces de aterosclerose, risco de recorrência de fibrilação atrial e mortalidade, principalmente por acidente vascular cerebral e infarto agudo do miocárdio, em pacientes com SAOS grave.Obstructive sleep apnea syndrome (OSAS is a common condition associated with various cardiovascular diseases, including systemic arterial hypertension, atrial fibrillation, and atherosclerosis. The association between OSAS and cardiovascular disease has been related to the overlapping of risk factors, including obesity, having a sedentary lifestyle, being male, and being older. However, there is mounting evidence that OSAS can also independently contribute to the development and progression of various cardiovascular diseases. The mechanisms by which OSAS can affect the cardiovascular system are multiple, including the activation of the sympathetic nervous system, systemic inflammation, insulin resistance, and oxidative stress. There is also evidence that the treatment of OSAS with CPAP can reduce arterial blood pressure, early signs of atherosclerosis, the risk of atrial fibrillation recurrence, and mortality (principally

  7. Successful Use of Orbital Atherectomy as an Adjunct in Treating Extensively Calcified Mesenteric Artery Lesions.

    Science.gov (United States)

    Richard, Michele; Krol, Emilia; Dietzek, Alan

    2016-10-01

    To describe the use of orbital technique of atherectomy as an adjunct to successful angioplasty and stent placement of the superior mesenteric artery (SMA). The technique is demonstrated in a 68-year-old man with critical SMA stenosis. The SMA was cannulated with 0.014-in wire, but the lesion was highly stenotic and densely calcified and prevented the passage of even the smallest 1.5-mm balloon. Orbital atherectomy was thus performed with a 1.25-mm CSI crown. Balloon angioplasty was then possible with a 4 mm × 2 cm balloon followed by placement of a 7 mm × 22 mm balloon-mounted stent. The use of atherectomy as an adjunct to angioplasty and stenting in extensive, calcified SMA lesions supports the value of this technique to avoid a much larger and morbid open procedure. Published by Elsevier Inc.

  8. Calcifying Cystic Odontogenic Tumour: immunohistochemical expression of matrix metalloproteinases, their inhibitors (TIMPs and RECK) and inducer (EMMPRIN).

    Science.gov (United States)

    Prosdócimi, Fábio C; Rodini, Camila O; Sogayar, Mari C; Sousa, Suzana C O M; Xavier, Flávia C A; Paiva, Katiúcia B S

    2014-08-01

    Calcifying cyst odontogenic tumour (CCOT) is a rare benign cystic neoplasm of odontogenic origin. MMPs are responsible for extracellular matrix remodelling and, together their inhibitors and inducer, determinate the level of its turnover in pathological processes, leading to an auspicious microenvironment for tumour development. Thus, our goal was to evaluate matrix metalloproteinases (MMPs-2, -7, -9 and -14), their inhibitors (TIMPs-2, -3, -4 and RECK) and its inductor (EMMPRIN) expression in CCOT. We used 18 cases of CCOT submitted to immunolocalization of the target proteins and analysed in both neoplastic odontogenic epithelial and stromal compartments. All molecules evaluated were expressed in both compartments in CCOT. In epithelial layer, immunostaining for MMPs, TIMPs, RECK and EMMPRIN was found in basal, suprabasal spindle and stellate cells surrounding ghost cells and ghost cells themselves, except for MMP-9 and TIMP-2 which were only expressed by ghost cells. In stromal compartment, extracellular matrix, mesenchymal (MC) and endothelial cells (EC) were positive for MMP-2, -7, TIMP-3 and -4, while MMP-9, TIMP-2 and RECK were positive only in MC and MMP-14 only in EC. Statistical significance difference was found between both compartments for MMP-9 (P EMMPRIN (P EMMPRIN and RECK expression was found (R = 0.661, P = 0.003). We concluded that these proteins/enzymes are differentially expressed in both epithelium and stroma of CCOT, suggesting an imbalance between MMPs and their inducer/inhibitors may contribute on the tumour behaviour. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. Energy balance of Sao Paulo State, Brazil 2012. Calendar year 2011

    International Nuclear Information System (INIS)

    2012-01-01

    The document presents the consolidated annual energy balances, which encompasses: Executive Summary; Participation of the Sao Paulo's Electric Energy in the National Context; Overview Energy Mix of the State of Sao Paulo; Final Consumption by Source; Final Consumption by Sectors in 2011; Contents of the Energy Balance for the State of Sao Paulo; Methodology; Summary of the period in analysis; Supply and Demand of energy by source; External trade of energy; Energy imports and export; Transformation center balances; Energy and Economy and Resources and energy Reserves. In this 2012 edition, we highlight the growth of gasoline consumption at the expense of ethanol, compared with previous years. The fall harvest of cane sugar and federal policies aimed at artificially support the price of gasoline are some of the factors that led to the fall in competitiveness of ethanol. Nowadays, it is up to the government to create policies to stimulate the supply of renewable fuels to replace fossil fuels. (author)

  10. Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet

    Directory of Open Access Journals (Sweden)

    Culley Nathan C

    2006-10-01

    Full Text Available Abstract Background It has been shown that calcifying vesicles play an important role in aortic calcification and that cholesterol content in the isolated vesicle fraction is increased when rabbits are fed supplemental cholesterol diets. Whether lipoprotein-associated cholesterols and other lipids are also increased in the vesicle fraction and whether the increase correlates with atherosclerosis remain unknown. Results Fourteen juvenile male rabbits fed an atherogenic diet containing 0.5% cholesterol and 2% peanut oil for 3 months developed varying degrees of hypercholesterolemia and intimal thickening in the ascending thoracic aorta. The correlation between these two parameters was insignificant, and likely attributable to the use of small numbers of rabbits in this study. Despite this lack of correlation, we demonstrate that the accumulation of cholesterol in calcifying vesicle fractions obtained from the collagenase-digested aorta fragments correlates well with intimal thickening (r2 = 0.98, p Conclusion When limited numbers of rabbits are used, LDL-C accumulation in calcifying vesicle fractions is a better biomarker for atherosclerosis than LDL-C levels in the serum. The close association of LDL-C with calcifying vesicles may play an important role in atherosclerosis and calcification.

  11. Calcified-tissue investigations using synchrotron x-ray microscopy

    International Nuclear Information System (INIS)

    Jones, K.W.; Spanne, P.; Schidlovsky, G.; Dejun, X.; Bockman, R.S.; Hammond, P.B.; Bornschein, R.L.; Hoeltzel, D.A.

    1990-10-01

    Synchrotron x-ray microscopy (SXRM) in both emission and absorption modes has been used to examine elemental distributions in specimens of rat tibia, human deciduous teeth, and an orthopedic implant phantom. The work was performed with a spatial resolution of 8 μm for the emission work and 25 μm for the absorption work. The results illustrate the usefulness of SXRM for measurements of different types of calcified tissue. 3 figs

  12. Calcifying algae maintain settlement cues to larval abalone following algal exposure to extreme ocean acidification.

    Science.gov (United States)

    O'Leary, Jennifer K; Barry, James P; Gabrielson, Paul W; Rogers-Bennett, Laura; Potts, Donald C; Palumbi, Stephen R; Micheli, Fiorenza

    2017-07-18

    Ocean acidification (OA) increasingly threatens marine systems, and is especially harmful to calcifying organisms. One important question is whether OA will alter species interactions. Crustose coralline algae (CCA) provide space and chemical cues for larval settlement. CCA have shown strongly negative responses to OA in previous studies, including disruption of settlement cues to corals. In California, CCA provide cues for seven species of harvested, threatened, and endangered abalone. We exposed four common CCA genera and a crustose calcifying red algae, Peyssonnelia (collectively CCRA) from California to three pCO 2 levels ranging from 419-2,013 µatm for four months. We then evaluated abalone (Haliotis rufescens) settlement under ambient conditions among the CCRA and non-algal controls that had been previously exposed to the pCO 2 treatments. Abalone settlement and metamorphosis increased from 11% in the absence of CCRA to 45-69% when CCRA were present, with minor variation among CCRA genera. Though all CCRA genera reduced growth during exposure to increased pCO 2 , abalone settlement was unaffected by prior CCRA exposure to increased pCO 2 . Thus, we find no impacts of OA exposure history on CCRA provision of settlement cues. Additionally, there appears to be functional redundancy in genera of CCRA providing cues to abalone, which may further buffer OA effects.

  13. {sup 137}Cs inventory in sedimentary columns from continental shelf of Sao Paulo state

    Energy Technology Data Exchange (ETDEWEB)

    Figueira, Rubens C.L.; Cordero, Luisa M.; Mahiques, Michel M. de; Tessler, Moyses G., E-mail: rfigueira@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. Oceanografico. Dept. de Oceanografia Fisica, Quimica e Geologica; Cruz, Jacson L.S. [Universidade Cruzeiro do Sul, Sao Paulo, SP (Brazil)

    2009-07-01

    {sup 137}Cs is an artificial radioactive isotope produced by {sup 235}U fission. This radionuclide has a high fission yield and a half-life of 30 years. It has been detected in the environment since 1945 and its principal contamination source has been nuclear tests in the atmosphere. There are other sources of {sup 137}Cs contamination in the environment, such as: release from nuclear and reprocessing plants, radioactive dumping and nuclear accidents (Chernobyl, for example). This paper presents an inventory of {sup 137}Cs on the Continental Shelf of Sao Paulo State, a region located between Cabo de Santa Marta Grande (Santa Catarina state) and Cabo Frio (Rio de Janeiro state). In this area, 9 cores were collected by the Instituto Oceanografico da Universidade de Sao Paulo (Sao Paulo University Institute of Oceanography). The cores were sliced at every 2 cm; sub-samples were lyophilized, grinded and stored in plastic containers. {sup 137}Cs was determined by 661 keV photopeak using a gamma spectrometry detector (Ge hyperpure). The analysis was performed by efficiency and background in different counting times. {sup 137}Cs concentration activities varied from 0.3 to 3.6 Bq kg{sup -1} with a mean value of 1.2+-0.6 Bq kg{sup -1}. The inventory of {sup 137}Cs in this area was 13+-7 Bq m-2. Values obtained are in agreement with the Southern Hemisphere, a region contaminated by atmospheric fallout due to past nuclear explosions. (author)

  14. Persistent Seroconversion after Accidental Eye Exposure to Calcifying Nanoparticles

    Science.gov (United States)

    Ciftcioglu, Neva; Aho, Katja M.; McKay, David S.; Kajander, E. Olavi

    2007-01-01

    Biosafety of nanomaterials has attracted much attention recently. We report here a case where accidental human eye exposure to biogenic nanosized calcium phosphate in the form of calcifying nanoparticles (CNP) raised a strong IgG immune response against proteins carried by CNP. The antibody titer has persisted over ten years at the high level. The IgG was detected by ELISA using CNPs propagated in media containing bovine and human serum as antigen. The exposure incident occurred to a woman scientist (WS) at a research laboratory in Finland at 1993. CNP, also termed "nanobacteria", is a unique self-replicating agent that has not been fully characterized and no data on biohazards were available at that time. Before the accident, her serum samples were negative for both CNP antigen and anti-CNP antibody using specific ELISA tests (Nanobac Oy, Kuopio, Finland). The accident occurred while WS was harvesting CNP cultures. Due to a high pressure in pipetting, CNP pellet splashed into her right eye. Both eyes were immediately washed with water and saline. The following days there was irritation and redness in the right eye. These symptoms disappeared within two weeks without any treatment. Three months after the accident, blood and urine samples of WS were tested for CNP cultures (2), CNP-specific ELISA tests, and blood cell counts. Blood cell counts were normal, CNP antigen and culture tests were negative. A high IgG anti-CNP antibody titer was detected (see Figure). The antibodies of this person have been used thereafter as positive control and standard in ELISA manufacturing (Nano-Sero IgG ELISA, Nanobac Oy, Kuopio, Finland).

  15. Hydro geochemistry of uranium in Aguas de Lindoia (Sao Paulo State)

    International Nuclear Information System (INIS)

    Silveira, E.G. da.

    1992-01-01

    A preliminary investigation concerning to the geochemical behaviour of uranium isotopes ( 233 U and 238 U) was performed on spring waters for Aguas de Lindoia city, Sao Paulo State. The reason for this study is because the natural radioactivity of these waters is poorly known, and no uranium content data have been published. Measurements of Uranium-238 contents and 234 U/ 238 U activity ratios in groundwaters were performed on the following springs issuing in the urban area from Aguas de Lindoia: Levissima I, Levissima II, Beleza, Sao Roque, Lindalia and Santa Izabel. (author)

  16. Mustard Gas Surrogate, 2-Chloroethyl Ethylsulfide (2-CEES), Induces Centrosome Amplification and Aneuploidy in Human and Mouse Cells

    Science.gov (United States)

    2014-03-01

    increase in aneuploidy in treated  cells .      Methods and Materials    Cell   Culture     Saos2 (human  osteosarcoma ) and NIH3T3 (murine embryonic...fibroblasts)  cells  were obtained  from ATCC (HTB‐85 and CRL‐1658, respectively) and  cultured  in complete media:  Dulbecco’s  Modified Eagle Medium (DMEM...subconfluent  cultures .  After the 5 day incubation,  cells  were treated     with 0.5 μg/ml colcemid (Gibco) for 4 hours.   Cell  media was harvested and retained

  17. Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell

    Directory of Open Access Journals (Sweden)

    Degnan Bernard M

    2010-11-01

    Full Text Available Abstract Background The formation of the molluscan shell is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell forming tissue, the mantle. This so called "calcifying matrix" is a complex mixture of proteins and glycoproteins that is assembled and occluded within the mineral phase during the calcification process. While the importance of the calcifying matrix to shell formation has long been appreciated, most of its protein components remain uncharacterised. Results Recent expressed sequence tag (EST investigations of the mantle tissue from the tropical abalone (Haliotis asinina provide an opportunity to further characterise the proteins in the shell by a proteomic approach. In this study, we have identified a total of 14 proteins from distinct calcified layers of the shell. Only two of these proteins have been previously characterised from abalone shells. Among the novel proteins are several glutamine- and methionine-rich motifs and hydrophobic glycine-, alanine- and acidic aspartate-rich domains. In addition, two of the new proteins contained Kunitz-like and WAP (whey acidic protein protease inhibitor domains. Conclusion This is one of the first comprehensive proteomic study of a molluscan shell, and should provide a platform for further characterization of matrix protein functions and interactions.

  18. δ11B as monitor of calcification site pH in divergent marine calcifying organisms

    Directory of Open Access Journals (Sweden)

    J. N. Sutton

    2018-03-01

    Full Text Available The boron isotope composition (δ11B of marine biogenic carbonates has been predominantly studied as a proxy for monitoring past changes in seawater pH and carbonate chemistry. However, a number of assumptions regarding chemical kinetics and thermodynamic isotope exchange reactions are required to derive seawater pH from δ11B biogenic carbonates. It is also probable that δ11B of biogenic carbonate reflects seawater pH at the organism's site of calcification, which may or may not reflect seawater pH. Here, we report the development of methodology for measuring the δ11B of biogenic carbonate samples at the multi-collector inductively coupled mass spectrometry facility at Ifremer (Plouzané, France and the evaluation of δ11BCaCO3 in a diverse range of marine calcifying organisms reared for 60 days in isothermal seawater (25 °C equilibrated with an atmospheric pCO2 of ca. 409 µatm. Average δ11BCaCO3 composition for all species evaluated in this study range from 16.27 to 35.09 ‰, including, in decreasing order, coralline red alga Neogoniolithion sp. (35.89 ± 3.71 ‰, temperate coral Oculina arbuscula (24.12 ± 0.19 ‰, serpulid worm Hydroides crucigera (19.26 ± 0.16 ‰, tropical urchin Eucidaris tribuloides (18.71 ± 0.26 ‰, temperate urchin Arbacia punctulata (16.28 ± 0.86 ‰, and temperate oyster Crassostrea virginica (16.03 ‰. These results are discussed in the context of each species' proposed mechanism of biocalcification and other factors that could influence skeletal and shell δ11B, including calcifying site pH, the proposed direct incorporation of isotopically enriched boric acid (instead of borate into biogenic calcium carbonate, and differences in shell/skeleton polymorph mineralogy. We conclude that the large inter-species variability in δ11BCaCO3 (ca. 20 ‰ and significant discrepancies between measured δ11BCaCO3 and δ11BCaCO3 expected from established relationships

  19. δ11B as monitor of calcification site pH in divergent marine calcifying organisms

    Science.gov (United States)

    Sutton, Jill N.; Liu, Yi-Wei; Ries, Justin B.; Guillermic, Maxence; Ponzevera, Emmanuel; Eagle, Robert A.

    2018-03-01

    The boron isotope composition (δ11B) of marine biogenic carbonates has been predominantly studied as a proxy for monitoring past changes in seawater pH and carbonate chemistry. However, a number of assumptions regarding chemical kinetics and thermodynamic isotope exchange reactions are required to derive seawater pH from δ11B biogenic carbonates. It is also probable that δ11B of biogenic carbonate reflects seawater pH at the organism's site of calcification, which may or may not reflect seawater pH. Here, we report the development of methodology for measuring the δ11B of biogenic carbonate samples at the multi-collector inductively coupled mass spectrometry facility at Ifremer (Plouzané, France) and the evaluation of δ11BCaCO3 in a diverse range of marine calcifying organisms reared for 60 days in isothermal seawater (25 °C) equilibrated with an atmospheric pCO2 of ca. 409 µatm. Average δ11BCaCO3 composition for all species evaluated in this study range from 16.27 to 35.09 ‰, including, in decreasing order, coralline red alga Neogoniolithion sp. (35.89 ± 3.71 ‰), temperate coral Oculina arbuscula (24.12 ± 0.19 ‰), serpulid worm Hydroides crucigera (19.26 ± 0.16 ‰), tropical urchin Eucidaris tribuloides (18.71 ± 0.26 ‰), temperate urchin Arbacia punctulata (16.28 ± 0.86 ‰), and temperate oyster Crassostrea virginica (16.03 ‰). These results are discussed in the context of each species' proposed mechanism of biocalcification and other factors that could influence skeletal and shell δ11B, including calcifying site pH, the proposed direct incorporation of isotopically enriched boric acid (instead of borate) into biogenic calcium carbonate, and differences in shell/skeleton polymorph mineralogy. We conclude that the large inter-species variability in δ11BCaCO3 (ca. 20 ‰) and significant discrepancies between measured δ11BCaCO3 and δ11BCaCO3 expected from established relationships between abiogenic δ11BCaCO3 and seawater pH arise

  20. Oil spill analysis in Sao Sebastiao Channel. Sao Paulo (SP) Brazil, from 1974 to 1994

    International Nuclear Information System (INIS)

    Poffo, Iris R.F.; Midaglia, Carmen L.; Heitzmann, Sueli R.; Caetano, Nelson; Cantao, Renato F.; Pompeia, Sergio L.; Eysink, Geraldo G.; Nakasaki, Akira

    1996-01-01

    Environmental accident files from PETROBRAS and CETEBS were studied. A total of 210 oil spill accidents were registered in Sao Sebastiao region (1974-1994), the oil reached the sea in 191 cases. The analysis was carried out by: cause of the accident, kind and amount of oil spilled, displacement of the oil spot, affected areas, dispersive used, amount of the oil and contaminated sands removes, waste disposal, and the relation between the wind direction intensity and the displacement of oil spot

  1. Cytotoxicity and Bioactivity of Calcium Silicate Cements Combined with Niobium Oxide in Different Cell Lines.

    Science.gov (United States)

    Mestieri, Leticia Boldrin; Gomes-Cornélio, Ana Lívia; Rodrigues, Elisandra Márcia; Faria, Gisele; Guerreiro-Tanomaru, Juliane Maria; Tanomaru-Filho, Mário

    2017-01-01

    The aim of this study was to evaluate the cytotoxicity and bioactivity of calcium silicate-based cements combined with niobium oxide (Nb2O5) micro and nanoparticles, comparing the response in different cell lines. This evaluation used four cell lines: two primary cultures (human dental pulp cells - hDPCs and human dental follicle cells - hDFCs) and two immortalized cultures (human osteoblast-like cells - Saos-2 and mouse periodontal ligament cells - mPDL). The tested materials were: White Portland Cement (PC), mineral trioxide aggregate (MTA), white Portland cement combined with microparticles (PC/Nb2O5µ) or nanoparticles (PC/Nb2O5n) of niobium oxide (Nb2O5). Cytotoxicity was evaluated by the methylthiazolyldiphenyl-tetrazolium bromide (MTT) and trypan blue exclusion assays and bioactivity by alkaline phosphatase (ALP) enzyme activity. Results were analyzed by ANOVA and Tukey test (a=0.05). PC/Nb2O5n presented similar or higher cell viability than PC/Nb2O5µ in all cell lines. Moreover, the materials presented similar or higher cell viability than MTA. Saos-2 exhibited high ALP activity, highlighting PC/Nb2O5µ material at 7 days of exposure. In conclusion, calcium silicate cements combined with micro and nanoparticles of Nb2O5 presented cytocompatibility and bioactivity, demonstrating the potential of Nb2O5 as an alternative radiopacifier agent for these cements. The different cell lines had similar response to cytotoxicity evaluation of calcium silicate cements. However, bioactivity was more accurately detected in human osteoblast-like cell line, Saos-2.

  2. Effects of Ocean Acidification and Temperature Increases on the Photosynthesis of Tropical Reef Calcified Macroalgae.

    Science.gov (United States)

    Scherner, Fernando; Pereira, Cristiano Macedo; Duarte, Gustavo; Horta, Paulo Antunes; E Castro, Clovis Barreira; Barufi, José Bonomi; Pereira, Sonia Maria Barreto

    2016-01-01

    Climate change is a global phenomenon that is considered an important threat to marine ecosystems. Ocean acidification and increased seawater temperatures are among the consequences of this phenomenon. The comprehension of the effects of these alterations on marine organisms, in particular on calcified macroalgae, is still modest despite its great importance. There are evidences that macroalgae inhabiting highly variable environments are relatively resilient to such changes. Thus, the aim of this study was to evaluate experimentally the effects of CO2-driven ocean acidification and temperature rises on the photosynthesis of calcified macroalgae inhabiting the intertidal region, a highly variable environment. The experiments were performed in a reef mesocosm in a tropical region on the Brazilian coast, using three species of frondose calcifying macroalgae (Halimeda cuneata, Padina gymnospora, and Tricleocarpa cylindrica) and crustose coralline algae. The acidification experiment consisted of three treatments with pH levels below those occurring in the region (-0.3, -0.6, -0.9). For the temperature experiment, three temperature levels above those occurring naturally in the region (+1, +2, +4°C) were determined. The results of the acidification experiment indicate an increase on the optimum quantum yield by T. cylindrica and a decline of this parameter by coralline algae, although both only occurred at the extreme acidification treatment (-0.9). The energy dissipation mechanisms of these algae were also altered at this extreme condition. Significant effects of the temperature experiment were limited to an enhancement of the photosynthetic performance by H. cuneata although only at a modest temperature increase (+1°C). In general, the results indicate a possible photosynthetic adaptation and/or acclimation of the studied macroalgae to the expected future ocean acidification and temperature rises, as separate factors. Such relative resilience may be a result of the

  3. The age of the rocks and the metamorphic episodes from the Southeastern of Sao Paulo state

    International Nuclear Information System (INIS)

    Tassinari, C.C.G.; Kawashita, K.; Schmuss, R. van; Taylor, P.N.

    1988-01-01

    Rb-Sr, Pb-Pb and U-Pb geochronologic studies carried out on precambrian rocks from the southeastern Sao Paulo state suggest a Complex geologic evolution during the Archean and Proterozoic times. This region is divided in five differents allochthonous terranes named Itapira-Amparo, Piracaiba-Jundiai, Sao Roque, Embu and Costeiro, separated by thrust and strike-slip faults. The Itapira-Amparo domain has a original history dating back to 3.4 Ga. and since 2.6 to 2.5 Ga. and 2.2 to 1.9 Ga. metamorphic rockformation episode occurred involving both mantle-derived magmas and recycled material. Supracrustal sequences developed around 1.4 Ga. The domain was locally reworked in 0.8 - 0.65 Ga. In the Piracaia-Jundiai the main rock-formation event occurred at 1.4 Ga., but this domian was affected by strong granization and migmatization episodes during the period 1.1 - 0.6 Ga. The third terrain is characterized by the Sao Roque metavolcanossedimentary sequence developed during the time period 1.8 - 0.7 Ga., and comprising two metamorphic superimposed events (1.4 and 0.8 - 0.7 Ga.). the post-tectonics granites ranging in ages from 0.7 to 0.55 Ga. Within the Embu terrain ages of 2.5, 1.4 and 0,75 Ga. were obtained for the metamorphic terrain, with post-tectonic activities around 650 Ma. In the Costeiro domain all the metamorphic rocks developed in late-Proterozoic time, with syntectonic phase around 650 Ma [pt

  4. Directional atherectomy of a heavy calcified axillary artery stenosis inducing critical hand ischemia.

    Science.gov (United States)

    Anzuini, Angelo; Palloshi, Altin; Aprigliano, Gianfranco; Ielasi, Alfonso

    2013-07-01

    The presence of a severe calcified peripheral artery lesion is responsible for a poor response to balloon dilation, due to significant acute vessel recoil and frequent flow-limiting dissections requiring stent implantation. This possibility could be associated with very high compression and/or fracture rates particularly in cases of lesion located at the mobile joints. In this setting directional atherectomy offers the theoretical advantages of eliminating stretch injury on arterial walls and reducing the restenosis rate by direct plaque excision. In this report, we present a case of critical hand ischemia due to a heavily calcified axillary artery lesion managed by directional atherectomy and balloon angioplasty followed by immediate angiographic success and sustained clinical benefit up to 3 years of follow-up.

  5. Primary breast osteosarcoma mimicking calcified fibroadenoma on screening digital breast tomosynthesis mammogram

    Directory of Open Access Journals (Sweden)

    Debbie Lee Bennett, MD

    2017-12-01

    Full Text Available Primary breast osteosarcoma is a rare malignancy, with mostly case reports in the literature. The appearance of breast osteosarcoma on digital breast tomosynthesis imaging has not yet been described. A 69-year-old woman presents for routine screening mammography and is found to have a calcified mass in her right breast. Pattern of calcification appeared “sunburst” on digital breast tomosynthesis images. This mass was larger than on the previous year's mammogram, at which time it had been interpreted as a benign calcified fibroadenoma. The subsequent workup demonstrated the mass to reflect primary breast osteosarcoma. The patient's workup and treatment are detailed in this case. Primary breast osteosarcoma, although rare, should be included as a diagnostic consideration for breast masses with a sunburst pattern of calcifications, particularly when the mammographic appearance has changed.

  6. Surface Airways Observations (SAO) Hourly Data (1965-1981) (CDMP)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The dataset consists primarily of U.S. surface airways observations (SAO) data beginning in 1965 and extending through 1981. Note that a few stations have already...

  7. Surface Airways Observations (SAO) Hourly Data 1928-1948 (CDMP)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The dataset consists of hourly U.S. surface airways observations (SAO). These observations extend as far back as 1928, from the time when commercial aviation began...

  8. Sao Paulo Lightning Mapping Array (SP-LMA): Deployment, Operation and Initial Data Analysis

    Science.gov (United States)

    Blakeslee, R.; Bailey, J. C.; Carey, L. D.; Rudlosky, S.; Goodman, S. J.; Albrecht, R.; Morales, C. A.; Anseimo, E. M.; Pinto, O.

    2012-01-01

    An 8-10 station Lightning Mapping Array (LMA) network is being deployed in the vicinity of Sao Paulo to create the SP-LMA for total lightning measurements in association with the international CHUVA [Cloud processes of the main precipitation systems in Brazil: A contribution to cloud resolving modeling and to the GPM (Global Precipitation Measurement)] field campaign. Besides supporting CHUVA science/mission objectives and the Sao Luiz do Paraitinga intensive operation period (IOP) in November-December 2011, the SP-LMA will support the generation of unique proxy data for the Geostationary Lightning Mapper (GLM) and Advanced Baseline Imager (ABI), both sensors on the NOAA Geostationary Operational Environmental Satellite-R (GOES-R), presently under development and scheduled for a 2015 launch. The proxy data will be used to develop and validate operational algorithms so that they will be ready for use on "day1" following the launch of GOES-R. A preliminary survey of potential sites in the vicinity of Sao Paulo was conducted in December 2009 and January 2010, followed up by a detailed survey in July 2010, with initial network deployment scheduled for October 2010. However, due to a delay in the Sao Luiz do Paraitinga IOP, the SP-LMA will now be installed in July 2011 and operated for one year. Spacing between stations is on the order of 15-30 km, with the network "diameter" being on the order of 30-40 km, which provides good 3-D lightning mapping 150 km from the network center. Optionally, 1-3 additional stations may be deployed in the vicinity of Sao Jos dos Campos.

  9. Cycling for Transportation in Sao Paulo City: Associations with Bike Paths, Train and Subway Stations.

    Science.gov (United States)

    Florindo, Alex Antonio; Barrozo, Ligia Vizeu; Turrell, Gavin; Barbosa, João Paulo Dos Anjos Souza; Cabral-Miranda, William; Cesar, Chester Luiz Galvão; Goldbaum, Moisés

    2018-03-21

    Cities that support cycling for transportation reap many public health benefits. However, the prevalence of this mode of transportation is low in Latin American countries and the association with facilities such as bike paths and train/subway stations have not been clarified. We conducted a cross-sectional analysis of the relationship between bike paths, train/subway stations and cycling for transportation in adults from the city of Sao Paulo. We used data from the Sao Paulo Health Survey ( n = 3145). Cycling for transportation was evaluated by a questionnaire and bike paths and train/subway stations were geocoded using the geographic coordinates of the adults' residential addresses in 1500-m buffers. We used multilevel logistic regression, taking account of clustering by census tract and households. The prevalence of cycling for transportation was low (5.1%), and was more prevalent in males, singles, those active in leisure time, and in people with bicycle ownership in their family. Cycling for transportation was associated with bike paths up to a distance of 500 m from residences (OR (Odds Ratio) = 2.54, 95% CI (Confidence interval) 1.16-5.54) and with the presence of train/subway stations for distances >500 m from residences (OR = 2.07, 95% CI 1.10-3.86). These results are important to support policies to improve cycling for transportation in megacities such as Sao Paulo.

  10. Geological and technological characteristics of the Ball Clay of the Sao Paulo state

    International Nuclear Information System (INIS)

    Tanno, L.C.; Motta, J.F.M.; Cabral Junior, M.; Saka, S.; Souza, D.D.D.

    1990-01-01

    This paper shows preliminary geological and technological results of studies about ball clay in Sao Paulo State. The works had been carried out by the Institute of Research and technology (IPT) and sponsored by Prominerio, during 88. Ball clay is a special clay utilised in the whiteware industry, mainly in the body preparation of sanitaryware products. This raw material come from two sites in Brazil: Sao Simao and Oeiras. Samples from these two deposits had been studied and classified acording to their adequately in the ceramic process. On the other hand, more than 100 samples from several geological sites of the Sao Paulo State were studied in laboratories. Acording to preliminary tests some of them revealed similar characteristics as brazilian ball clays. These clays were characterized by granulometry analysis, X-ray diffraction and chemical analysis. (author) [pt

  11. Coral calcifying fluid pH dictates response to ocean acidification.

    Science.gov (United States)

    Holcomb, M; Venn, A A; Tambutté, E; Tambutté, S; Allemand, D; Trotter, J; McCulloch, M

    2014-06-06

    Ocean acidification driven by rising levels of CO2 impairs calcification, threatening coral reef growth. Predicting how corals respond to CO2 requires a better understanding of how calcification is controlled. Here we show how spatial variations in the pH of the internal calcifying fluid (pHcf) in coral (Stylophora pistillata) colonies correlates with differential sensitivity of calcification to acidification. Coral apexes had the highest pHcf and experienced the smallest changes in pHcf in response to acidification. Lateral growth was associated with lower pHcf and greater changes with acidification. Calcification showed a pattern similar to pHcf, with lateral growth being more strongly affected by acidification than apical. Regulation of pHcf is therefore spatially variable within a coral and critical to determining the sensitivity of calcification to ocean acidification.

  12. Distribution of Th-232 and Th-228 in Santos and Sao Vicente Estuary, Sao Paulo, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Silva, P.S.C.; Mazzilli, B.P.; Favaro, D.I.T. [Instituto de Pesquisas Energeticas e Nucleares, Av. Lineu Prestes, 2242, Caixa Postal 05508-000, Cidade Universitaria, Sao Paulo (Brazil)]. e-mail: Pscsilva@ipen.br

    2004-07-01

    In the last decades considerable attention has been given to technologically enhanced natural occurring radioactive material (TENORM). Within this frame, of particular concern is the phosphate fertilizer industry. Santos Basin, located in Southwest Brazil, Sao Paulo State, comprising Santos and Sao Vicente estuarine system, is considered the most important industrial region of the country. Among the industrial activities present, phosphate fertilizer plants are responsible for the production of 69 millions tons of phosphogypsum waste, which is stockpiled in the surrounding environment. This waste concentrates radionuclides of the natural series originally present in the phosphate rock used as raw material. This study aims to evaluate the environmental impact of such activities in the sediments of the estuarine system. {sup 232} Th and {sup 228} Th concentrations in Santos and San Vicente estuary sediments were determined by Neutron activation analysis and Gamma spectrometry, respectively. {sup 232} Th concentration ranged from 6.5 to 198 Bq kg{sup -1} with mean value of 57 {+-} 39 Bq kg{sup -1}, for 42 samples. {sup 228} Th content varied from 12 to 110 Bq kg{sup -1} with a mean value of 74 {+-} 23 Bq kg{sup -1}, for 18 samples. It can be seen that the amount of {sup 232} Th is higher in the rivers close to the phosphogypsum piles, at least five points were identified as being affected by anthropogenic factor. (Author)

  13. Distribution of Th-232 and Th-228 in Santos and Sao Vicente Estuary, Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Silva, P.S.C.; Mazzilli, B.P.; Favaro, D.I.T.

    2004-01-01

    In the last decades considerable attention has been given to technologically enhanced natural occurring radioactive material (TENORM). Within this frame, of particular concern is the phosphate fertilizer industry. Santos Basin, located in Southwest Brazil, Sao Paulo State, comprising Santos and Sao Vicente estuarine system, is considered the most important industrial region of the country. Among the industrial activities present, phosphate fertilizer plants are responsible for the production of 69 millions tons of phosphogypsum waste, which is stockpiled in the surrounding environment. This waste concentrates radionuclides of the natural series originally present in the phosphate rock used as raw material. This study aims to evaluate the environmental impact of such activities in the sediments of the estuarine system. 232 Th and 228 Th concentrations in Santos and San Vicente estuary sediments were determined by Neutron activation analysis and Gamma spectrometry, respectively. 232 Th concentration ranged from 6.5 to 198 Bq kg -1 with mean value of 57 ± 39 Bq kg -1 , for 42 samples. 228 Th content varied from 12 to 110 Bq kg -1 with a mean value of 74 ± 23 Bq kg -1 , for 18 samples. It can be seen that the amount of 232 Th is higher in the rivers close to the phosphogypsum piles, at least five points were identified as being affected by anthropogenic factor. (Author)

  14. Metal assessment in urban park soils in Sao Paulo. 3. Aclimacao Park

    International Nuclear Information System (INIS)

    Pavese, Arthur C.; Figueiredo, Ana Maria G.; Camargo, Sonia P.; Gumiero, Felipe C.; Enzweiler, Jacinta

    2007-01-01

    As part of a project which aims metal assessment in urban park soils from Sao Paulo, in the present paper the concentration of the elements As, Ba, Zn, Sb, Se, Co, Cr, Cu and Pb were determined in surface soil samples (0-5 cm and 0-20 cm) from Aclimacao park of Sao Paulo. Urban soils play an important role in maintaining the environmental quality as they can act as both source and sink for pollutants that can affect human health. Parks and playgrounds are where urban children spend most of their time outdoors and are also where children most frequently come in contact with soil. Aclimacao park is located at the central region of the city, in a residential area. Instrumental Neutron Activation Analysis (INAA) and X-Ray Fluorescence (XRF) were used for metal analysis. The results obtained for Zn were higher than the values considered as reference values for soils in Sao Paulo, according to the Environmental Protection Agency of the State of Sao Paulo (CETESB), but lower than the Prevention values. For Ba, Cr, and Sb, the results obtained showed concentration levels higher than Prevention value reported by CETESB. According to CETESB, metal concentration levels above the Prevention value can cause prejudicial alterations in soil and subterranean water quality. For As, in the 0-5 cm samples, the concentration levels were near or above the Intervention value for agricultural area reported by CETESB. (author)

  15. ORBIT II sub-analysis: Impact of impaired renal function following treatment of severely calcified coronary lesions with the Orbital Atherectomy System.

    Science.gov (United States)

    Lee, Michael S; Lee, Arthur C; Shlofmitz, Richard A; Martinsen, Brad J; Hargus, Nick J; Elder, Mahir D; Généreux, Philippe; Chambers, Jeffrey W

    2017-04-01

    To investigate the safety and efficacy of the coronary Orbital Atherectomy System (OAS) to prepare severely calcified lesions for stent deployment in patients grouped by renal function. Percutaneous coronary intervention (PCI) of severely calcified lesions is associated with increased rates of major adverse cardiac events (MACE), including death, myocardial infarction (MI), and target vessel revascularization (TVR) compared with PCI of non-calcified vessels. Patients with chronic kidney disease (CKD) are at increased risk for MACE after PCI. The impact of CKD on coronary orbital atherectomy treatment has not been well characterized. ORBIT II was a prospective, multicenter trial in the U.S., which enrolled 443 patients with severely calcified coronary lesions. The MACE rate was defined as a composite of cardiac death, MI, and target vessel revascularization. Of the 441 patients enrolled with known estimated glomerular filtration rate (eGFR) values at baseline, 333 (75.5%) patients had eGFR renal impairment had a higher MACE rate through one year follow-up due to a higher rate of periprocedural MI. Interestingly, the rates of cardiac death and revascularization through 1-year were similar in patients with eGFR renal impairment and severely calcified coronary lesions. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  16. Simultaneous rota-stenting and transcatheter aortic valve implantation for patients with heavily calcified coronary stenosis and aortic stenosis

    Directory of Open Access Journals (Sweden)

    Yung-Tsai Lee

    2016-09-01

    Full Text Available Given that coronary artery disease (CAD in octogenarians undergoing transcatheter aortic valve implantation (TAVI often presents with more complex lesions and extensive calcification, rotational atherectomy (RA may be needed in some cases before stenting. However, data regarding the feasibility and safety of simultaneous RA during TAVI using the Medtronic CoreValve (MCV; Medtronic, Minneapolis, MN, USA system are lacking. Three out of 107 (2.8% patients (2 females, average age 85.6 years, mean aortic valve area 0.5 cm2, mean left ventricular ejection fraction 39%, mean Logistic EuroScore 70%, with complex, heavily calcified coronary stenosis, and severe valvular aortic stenosis (AS were treated with TAVI and RA due to high surgical risk. After balloon valvuloplasty, all coronary lesions were successfully treated with RA and stenting, immediately followed by transfemoral TAVI with a self-expandable MCV. Our data suggested that in the very elderly patients with severe and heavily calcified CAD and AS who were turned down for cardiac surgery, RA and stenting followed by TAVI may be performed successfully in a combined, single-stage procedure.

  17. Origin and evolution of the Pirituba aluminous calc-alkalic magma, state of Sao Paulo, SE Brazil

    International Nuclear Information System (INIS)

    Wernick, Eberhard

    1999-01-01

    The origin and evolution of the Late Proterozoic calc-alkalic Pirituba magmatism near the city of Sao Paulo, State of Sao Paulo, SE Brazil, are presented and discussed on the basis of geological, petrographic, mineralogical, chemical (rocks and minerals), isotopic and zircon typology data. (author)

  18. Calcified fibrous pseudotumor of spermatic cord

    International Nuclear Information System (INIS)

    Rodriguez Collar, Tomas Lazaro; Valdes Estevez, Brasily; Nagua Valencia, Miguel Angel; Salinas Olivares, Mercedes Rita

    2009-01-01

    Paratesticular tumors are infrequent and most are benigns. This a case presentation of a patient aged 24 with a history of good health coming to our consultation by presence of a hard and painless 4 cm tumor in right scrotum with a 6 months course. Scrotal ultrasound (US) showed a well circumscribed heterogeneous lesion separate of epididymis and the testis. Fine needle aspiration biopsy (FNAB) was not possible by hardness of tumor. A inguinal surgery was performed and the total tumor exeresis using freezing biopsy negative of malignancy. Final histopathological report was: calcified fibrous pseudotumor of spermatic cord. Patient's course has been satisfactory. Significance of inguinal surgery was confirmed for the paratesticular tumor approach, even more when FNAB it is not conclusive for diagnosis.(author)

  19. Valve-Like and Protruding Calcified Intimal Flap Complicating Common Iliac Arteries Kissing Stenting

    Directory of Open Access Journals (Sweden)

    George S. Georgiadis

    2015-01-01

    Full Text Available Endovascular therapy for iliac artery chronic total occlusions is nowadays associated with low rates of procedure-related complications and improved clinical outcomes, and it is predominantly used as first-line therapy prior to aortobifemoral bypass grafting. Herein, we describe the case of a patient presenting with an ischemic left foot digit ulcer and suffering complex aortoiliac lesions, who received common iliac arteries kissing stents, illustrating at final antegrade and retrograde angiograms the early recognition of a blood flow obstructing valve-like calcified intimal flap protruding through the stent struts, which was obstructing antegrade but not retrograde unilateral iliac arterial axis blood flow. The problem was resolved by reconstructing the aortic bifurcation at a more proximal level. Completion angiogram verified normal patency of aorta and iliac vessels. Additionally, a severe left femoral bifurcation stenosis was also corrected by endarterectomy-arterioplasty with a bovine patch. Postintervention ankle brachial pressure indices were significantly improved. At the 6-month and 2-year follow-up, normal peripheral pulses were still reported without intermittent claudication suggesting the durability of the procedure. Through stent-protruding calcified intimal flap, is a very rare, but existing source of antegrade blood flow obstruction after common iliac arteries kissing stents.

  20. Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves

    Directory of Open Access Journals (Sweden)

    Najlah Kochtebane

    2014-01-01

    Full Text Available Purpose. Our physiopathological assumption is that u-PA, t-PA, and PAI-1 are released by calcified aortic valves and play a role in the calcification of these valves. Methods. Sixty-five calcified aortic valves were collected from patients suffering from aortic stenosis. Each valve was incubated for 24 hours in culture medium. The supernatants were used to measure u-PA, t-PA, and PAI-1 concentrations; the valve calcification was evaluated using biphotonic absorptiometry. Results. Aortic stenosis valves expressed normal plasminogen activators concentrations and overexpressed PAI-1 (u-PA, t-PA, and PAI-1 mean concentrations were, resp., 1.69 ng/mL ± 0.80, 2.76 ng/mL ± 1.33, and 53.27 ng/mL ± 36.39. There was no correlation between u-PA and PAI-1 (r=0.3 but t-PA and PAI-1 were strongly correlated with each other (r=0.6. Overexpression of PAI-1 was proportional to the calcium content of the AS valves. Conclusions. Our results demonstrate a consistent increase of PAI-1 proportional to the calcification. The overexpression of PAI-1 may be useful as a predictive indicator in patients with aortic stenosis.

  1. Framework for detection and localization of coronary non-calcified plaques in cardiac CTA using mean radial profiles.

    Science.gov (United States)

    Jawaid, Muhammad Moazzam; Riaz, Atif; Rajani, Ronak; Reyes-Aldasoro, Constantino Carlos; Slabaugh, Greg

    2017-10-01

    The high mortality rate associated with coronary heart disease (CHD) has driven intensive research in cardiac imaging and image analysis. The advent of computed tomography angiography (CTA) has turned non-invasive diagnosis of cardiovascular anomalies into reality as calcified coronary plaques can be easily identified due to their high intensity values. However, the detection of non-calcified plaques in CTA is still a challenging problem because of lower intensity values, which are often similar to the nearby blood and muscle tissues. In this work, we propose the use of mean radial profiles for the detection of non-calcified plaques in CTA imagery. Accordingly, we computed radial profiles by averaging the image intensity in concentric rings around the vessel centreline in a first stage. In the subsequent stage, an SVM classifier is applied to identify the abnormal coronary segments. For occluded segments, we further propose a derivative-based method to localize the position and length of the plaque inside the segment. A total of 32 CTA volumes were analysed and a detection accuracy of 88.4% with respect to the manual expert was achieved. The plaque localization accuracy was computed using the Dice similarity coefficient and a mean of 83.2% was achieved. The consistent performance for multi-vendor, multi-institution data demonstrates the reproducibility of our method across different CTA datasets with a good agreement with manual expert annotations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Viability of calcifying bacterial formulations in fly ash for applications in building materials.

    Science.gov (United States)

    Dhami, Navdeep Kaur; Mukherjee, Abhijit; Reddy, M Sudhakara

    2013-12-01

    Evidence of bacterial involvement in precipitation of calcium carbonates has brought a revolution in the field of applied microbiology, geotechnical sciences, environmental and civil engineering with its marked success in restoration of various building materials. For applications of these calcite binder-producing bacterial cultures, different expensive carrier materials have been used but their high costs have come in the way of their successful commercialization. In the present study, we have explored the potential of cheap industrial by-product fly ash as a carrier material for bacterial cells and investigated the viability of calcifying bacterial isolates: Bacillus megaterium, Bacillus cereus, and Lysinibacillus fusiformis in fly ash carrier at varying temperatures and moisture conditions along with biomineralization efficacy of these formulations. We used laser scanning confocal microscopy to analyze the viability of bacteria by florescent dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) along with the plate count method. Results revealed that fly ash successfully served as an effective carrier material and bacterial formulations stored at 4 °C provided longer shelf life than those stored at higher temperatures. Up to 10(6) cfu/g was found to sustain in all formulations at 4 °C compared to 10(4)-10(5) cfu/g in case of higher temperatures up to 1 year. For 4 °C, higher moistures (50 %) were found to provide better survivability while for higher temperatures, lower moistures (30 %) favored higher viability. The biomineralization capability of fresh and formulated bacterial cells was compared on the basis of precipitation of carbonates and it was found that carbonate precipitation efficacy of formulated bacterial cells was comparable to fresh bacterial cells.

  3. Challenges for energy efficiency in the buildings sector in the Sao Paulo State, Brazil; Desafios para eficiencia energetica no setor de edificios no estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Matos, Maria Ines; Parente, Virginia [Universidade de Sao Paulo (IEE/USP), SP (Brazil). Inst. de Eletrotecnica e Energia

    2010-07-01

    The consumption of energy in the building sector accounts for more than one third of the total final electricity consumption in Sao Paulo State. Considering that, the development of policy measures aiming at the promotion of energy efficiency in the sector should be encouraged. Analysed data reveals the continuing rise of energy consumption resulting form the high number of new buildings and the rising standards of the population in consequence of economic development. Besides this, the retrofit of old buildings presents a huge potential for energy savings. The article analyses and suggests the use of Building Codes as a suitable policy instrument for the Sao Paulo Sate reality. (author)

  4. Impaired STING Pathway in Human Osteosarcoma U2OS Cells Contributes to the Growth of ICP0-Null Mutant Herpes Simplex Virus.

    Science.gov (United States)

    Deschamps, Thibaut; Kalamvoki, Maria

    2017-05-01

    Human herpes simplex virus 1 (HSV-1) is a widespread pathogen, with 80% of the population being latently infected. To successfully evade the host, the virus has evolved strategies to counteract antiviral responses, including the gene-silencing and innate immunity machineries. The immediately early protein of the virus, infected cell protein 0 (ICP0), plays a central role in these processes. ICP0 blocks innate immunity, and one mechanism is by degrading hostile factors with its intrinsic E3 ligase activity. ICP0 also functions as a promiscuous transactivator, and it blocks repressor complexes to enable viral gene transcription. For these reasons, the growth of a ΔICP0 virus is impaired in most cells, except cells of the human osteosarcoma cell line U2OS, and it is only partially impaired in cells of the human osteosarcoma cell line Saos-2. We found that the two human osteosarcoma cell lines that supported the growth of the ΔICP0 virus failed to activate innate immune responses upon treatment with 2'3'-cyclic GAMP (2'3'-cGAMP), the natural agonist of STING (i.e., stimulator of interferon genes) or after infection with the ΔICP0 mutant virus. Innate immune responses were restored in these cells by transient expression of the STING protein but not after overexpression of interferon-inducible protein 16 (IFI16). Restoration of STING expression resulted in suppression of ΔICP0 virus gene expression and a decrease in viral yields. Overexpression of IFI16 also suppressed ΔICP0 virus gene expression, albeit to a lesser extent than STING. These data suggest that the susceptibility of U2OS and Saos-2 cells to the ΔICP0 HSV-1 is in part due to an impaired STING pathway. IMPORTANCE The DNA sensor STING plays pivotal role in controlling HSV-1 infection both in cell culture and in mice. The HSV-1 genome encodes numerous proteins that are dedicated to combat host antiviral responses. The immediate early protein of the virus ICP0 plays major role in this process as it targets

  5. Two-Year Clinical Outcomes of Newer-Generation Drug-Eluting Stent Implantation Following Rotational Atherectomy for Heavily Calcified Lesions.

    Science.gov (United States)

    Jinnouchi, Hiroyuki; Kuramitsu, Shoichi; Shinozaki, Tomohiro; Kobayashi, Yohei; Hiromasa, Takashi; Morinaga, Takashi; Mazaki, Toru; Sakakura, Kenichi; Soga, Yoshimitsu; Hyodo, Makoto; Shirai, Shinichi; Ando, Kenji

    2015-01-01

    Clinical outcomes of implantation of the newer-generation drug-eluting stent (DES) following rotational atherectomy for heavily calcified lesions remain unclear in the real-world setting. We enrolled 252 consecutive patients (273 lesions) treated with newer-generation DES following rotational atherectomy. The primary endpoint was the cumulative 2-year incidence of major adverse cardiovascular events (MACE), defined as cardiac death, myocardial infarction, clinically-driven target lesion revascularization, and definite stent thrombosis. Complete clinical follow-up information at 2-year was obtained for all patients. The mean age was 73.2±9.0 years and 155 patients (61.5%) were male. Cumulative 2-year incidence of MACE (cardiac death, myocardial infarction, clinically-driven target lesion revascularization and definite stent thrombosis) was 20.3% (7.0%, 2.1%, 18.1% and 2.1%, respectively). Predictors of MACE were presenting with acute coronary syndrome (hazard ratio [HR]: 3.80, 95% confidence interval [CI]: 1.29-11.2, P=0.02), hemodialysis (HR: 1.93, 95% CI: 1.04-3.56, P=0.04) and previous coronary artery bypass graft (HR: 2.26, 95% CI: 1.02-5.00, P=0.045). PCI for calcified lesions requiring rotational atherectomy is still challenging even in the era of newer-generation DES.

  6. Trace elements in the urban aerosol of Sao Paulo

    International Nuclear Information System (INIS)

    Orsini, C.M.Q.; Artaxo Netto, P.E.; Tabacniks, M.H.

    1983-07-01

    The first trace-element data-set measured on the atmosphere aerosol of Sao Paulo City, Brazil, is presented. These measurements have been based on samplings by 6-stage cascade impactors combined with the analytical procedure by the PIXE method of elementary analysis. (L.C.) [pt

  7. Interaction of Protein Phosphatase 1δ with Nucleophosmin in Human Osteoblastic Cells

    International Nuclear Information System (INIS)

    Haneji, Tatsuji; Teramachi, Jumpei; Hirashima, Kanji; Kimura, Koji; Morimoto, Hiroyuki

    2012-01-01

    Protein phosphorylation and dephosphorylation has been recognized as an essential mechanism in the regulation of cellular metabolism and function in various tissues. Serine and threonine protein phosphatases (PP) are divided into four categories: PP1, PP2A, PP2B, and PP2C. At least four isoforms of PP1 catalytic subunit in rat, PP1α, PP1γ1, PP1γ2, and PP1δ, were isolated. In the present study, we examined the localization and expression of PP1δ in human osteoblastic Saos-2 cells. Anti-PP1δ antibody recognized a protein present in the nucleolar regions in Saos-2 cells. Cellular fractionation revealed that PP1δ is a 37 kDa protein localized in the nucleolus. Nucleophosmin is a nucleolar phosphoprotein and located mainly in the nucleolus. Staining pattern of nucleophosmin in Saos-2 cells was similar to that of PP1δ. PP1δ and nucleophosmin were specifically stained as dots in the nucleus. Dual fluorescence images revealed that PP1δ and nucleophosmin were localized in the same regions in the nucleolus. Similar distribution patterns of PP1δ and nucleophosmin were observed in osteoblastic MG63 cells. The interaction of PP1δ and nucleophosmin was also shown by immunoprecipitation and Western analysis. These results indicated that PP1δ associate with nucleophosmin directly in the nucleolus and suggested that nucleophosmin is one of the candidate substrate for PP1δ

  8. The potential cost-effectiveness of the Diamondback 360® Coronary Orbital Atherectomy System for treating de novo, severely calcified coronary lesions: an economic modeling approach

    Science.gov (United States)

    Chambers, Jeffrey; Généreux, Philippe; Lee, Arthur; Lewin, Jack; Young, Christopher; Crittendon, Janna; Mann, Marita; Garrison, Louis P.

    2015-01-01

    Background: Patients who undergo percutaneous coronary intervention (PCI) for severely calcified coronary lesions have long been known to have worse clinical and economic outcomes than patients with no or mildly calcified lesions. We sought to assess the likely cost-effectiveness of using the Diamondback 360® Orbital Atherectomy System (OAS) in the treatment of de novo, severely calcified lesions from a health-system perspective. Methods and results: In the absence of a head-to-head trial and long-term follow up, cost-effectiveness was based on a modeled synthesis of clinical and economic data. A cost-effectiveness model was used to project the likely economic impact. To estimate the net cost impact, the cost of using the OAS technology in elderly (⩾ 65 years) Medicare patients with de novo severely calcified lesions was compared with cost offsets. Elderly OAS patients from the ORBIT II trial (Evaluate the Safety and Efficacy of OAS in Treating Severely Calcified Coronary Lesions) [ClinicalTrials.gov identifier: NCT01092426] were indirectly compared with similar patients using observational data. For the index procedure, the comparison was with Medicare data, and for both revascularization and cardiac death in the following year, the comparison was with a pooled analysis of the Harmonizing Outcomes with Revascularization and Stents in Acute Myocardial Infarction (HORIZONS-AMI)/Acute Catheterization and Urgent Intervention Triage Strategy (ACUITY) trials. After adjusting for differences in age, gender, and comorbidities, the ORBIT II mean index procedure costs were 17% (p < 0.001) lower, approximately US$2700. Estimated mean revascularization costs were lower by US$1240 in the base case. These cost offsets in the first year, on average, fully cover the cost of the device with an additional 1.2% cost savings. Even in the low-value scenario, the use of the OAS is cost-effective with a cost per life-year gained of US$11,895. Conclusions: Based on economic modeling

  9. Distal Renal Tubular Acidosis (dRTA) Among Southeast Asian Ovalocytosis (SAO) Patients in Malaria Endemic Area of Sekotong, Lombok Island

    OpenAIRE

    Danuyanti, I Gusti Ayu Nyoman; -, Tasmini; Sadewa, Ahmad Hamim

    2014-01-01

    Introduction: Southeast Asian Ovalocytosis (SAO) is caused by 27 bp deletion of the band 3 protein gene in erythrocyte membrane and characterized by oval erythrocyte. The erythroid band 3 (AE1) gene isexpressed not only in erythrocyte membranes but also in the cell membrane of α-collecting renal tubular functions in the secretion of acid in renal tubules and HCO3 -/Cl- anion exchange. An alteration of the band 3 (AE1) gene functions in cell of α-collecting renal tubules reduces HCO3-/Cl- ion ...

  10. Energy balance of the Sao Paulo State - 1991

    International Nuclear Information System (INIS)

    1993-01-01

    The energy production and consumption for the several categories of the economy in the Brazilian State of Sao Paulo are presented. The data are given according to the energy source and to consumer sectors and information about the import, export, losses in the transformation and distribution of the several forms of energy is supplied. 18 figs., 120 tabs

  11. Preliminary Evaluation of Microbial Communities Isolated from the Calcifying Fluid of Oysters

    Science.gov (United States)

    Banker, R.

    2016-02-01

    The process of biomineralization is defined as the selective uptake of elements that are incorporated into a defined mineral structure under strict biological control. For bivalve molluscs, such as clams, oysters, and mussels, the mantle is the primary organ in control of shell deposition. Alternatively, remote calcification takes place when carbonate-precipitating microbes (e.g. sulfate reducers) colonize a shell-secreting organism and enhance the ability of the host to build shell material. The oyster syndrome is a term that describes bivalves that possess an unusual shell morphology characterized by exceptionally thick valves containing numerous chambers filled with chalky calcite. Although remote calcification via microbial metabolism has been proposed as a mechanism of chalky deposit formation in oysters, this hypothesis has not yet been rigorously investigated. Here I present data on the microbial communities found in the calcifying fluid of two oyster species; Crassostrea gigas and Ostrea lurida are examples of oysters that do and do not exhibit the oyster syndrome, respectively. Comparison of the microbiomes of these two morphological end members may provide insight into the role of microbes in the formation of chalky deposits. Results indicate that the microbial community in the surrounding water is the dominant source for bacterial taxa found in the calcifying fluid of both oyster species. Also, it appears as though C. gigas maintains a microbial community that is more similar to its ambient environment than O. lurida. These results demonstrate that the ambient aquatic environment has a guiding influence on the microbiome found in the calcifying fluid of bivalve molluscs. However, the magnitude of this effect varies among organisms, even those that are closely related.

  12. Hydrogeological impacts evaluation like a subsidy for the hydroelectric reservoir installation in the Sao Paulo State, Brazil

    International Nuclear Information System (INIS)

    Albuquerque Filho, Jose Luiz; Bottura, Joao Alberto; Borin Junior, Tarcisio; Correa, Wilson Aparecido Garcia

    1994-01-01

    Hydrogeological studies developed in the influence areas of the big reservoirs at the Sao Paulo State, Sao Paulo/Mato Grosso do Sul and Sao Paulo/Parana, dedicated to the pre-filling situation evaluation, facilitated the preventive, corrective or alleviative actions adoption of the possible alterations in the adjacent free aquifers and, consequently, in the land use and occupation, as well as the planning of the rational usage of non-occupied areas in the reservoir margin. It presents the origin, the history and the objectives of the studies as well as the foreseen and confirmed hydrogeological impacts in monitored reservoirs

  13. The cell biology and role of resorptive cells in diseases: A review.

    Science.gov (United States)

    Babaji, Prashant; Devanna, Raghu; Jagtap, Kiran; Chaurasia, Vishwajit Rampratap; Jerry, Jeethu John; Choudhury, Basanta Kumar; Duhan, Dinesh

    2017-01-01

    Resorptive cells are responsible for the resorption of mineralized matrix of hard tissues. Bone-resorbing cells are called osteoclasts; however, they can resorb mineralized dental tissues or calcified cartilage and then they are called odontoclasts and chondroclasts, respectively. Resorptive cells form when mononuclear precursors derived from a monocyte-macrophage cell lineage are attracted to certain mineralized surfaces and subsequently fuse and adhere onto them for exerting their resorbing activity. These cells are responsible for degradation of calcified extracellular matrix composed of organic molecules and hydroxyapatite. The activity of these cells can be observed in both physiological and pathological processes throughout life and their activity is mainly required in bone turnover and growth, spontaneous and induced (orthodontic) tooth movement, tooth eruption, and bone fracture healing, as well as in pathological conditions such as osteoporosis, osteoarthritis, and bone metastasis. In addition, they are responsible for daily control of calcium homeostasis. Clastic cells also resorb the primary teeth for shedding before the permanent teeth erupt into the oral cavity.

  14. Atmospheric pollution biomonitoring of the Sao Paulo metropolitan region using epiphytic lichens; Uso de liquens epifiticos no biomonitoramento da poluicao atmosferica da regiao metropolitana de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Fuga, Alessandra

    2006-07-01

    Due to the increasing problems of atmospheric pollution in the Sao Paulo metropolitan region that affect the environment and human health the application of biomonitoring methodologies using cosmopolite organisms has now become relevant. Biomonitoring is a method to evaluate the response of live organisms to pollution. This method offers advantages such as reduced costs, efficient monitoring of large geographic areas and accumulated pollutants over a large period in which low concentrations of chemicals elements in the environment can be evaluated. In the present study, neutron activation analysis method was applied to determine elements accumulated in Canoparmelia texana lichenized fungi. Samples were collected in two distinct areas: Carlos Botelho (PECB) and Intervales (PEI) State Parks that are considered as non-polluted areas and that belong to the Atlantic Forest - SP ecosystem; and Sao Paulo city metropolitan region in sites near automatic monitoring stations of the Environmental Protection Agency of the State of Sao Paulo (CETESB). The lichens collected from the bark of the trees were properly treated, and irradiated with neutrons from IEA-R1 nuclear reactor along with synthetic standards of elements. The precision and the accuracy of the results were evaluated by the analyses of IAEA-336 LICHEN and Mixed Polish Herbs (INCT -MPH-2) certified reference materials. The results obtained for these materials were in accordance with the certified values and presented good precision with variation coefficients ranging from 0.9 to 14.6%. Results obtained for lichens showed that elements As, Co, Cr, Cs, La, Mo, Sb, Sc, Se and U are present at ng g{sup -1} levels, Ba, Br, Cl, Fe, K, Mn, Na, Rb and Zn at {mu}g g{sup -1} and Ca at mg g{sup -1}. By applying cluster and discriminant analyses to the results for the lichen samples from areas with different levels of pollution, the sampling sites were grouped according to their chemical similarities and their elemental

  15. Aspectos genéticos da SAOS Genetic aspects of obstructive sleep apnea syndrome

    Directory of Open Access Journals (Sweden)

    Adriane C. Mesquita Petruco

    2010-06-01

    Full Text Available A fisiopatologia da SAOS é resultante da interação entre fatores genéticos e ambientais. Os mais importantes fatores de risco são obesidade e idade. Outros fatores relevantes são anormalidades craniofaciais, hipotireoidismo, menopausa e uso de álcool e de sedativos. A hereditariedade tem sido relacionada a SAOS pela a associação de SAOS a níveis de HLA, obesidade, síndromes genéticas, etnias, sonolência excessiva, alteração do controle ventilatório, expressão de mediadores inflamatórios, entre outros. Este capítulo aborda a variabilidade genética e fenotípica da doença, demonstrando sua relevância no entendimento da fisiopatologia e na avaliação clínica de SAOS.The physiopathology of obstructive sleep apnea syndrome (OSAS results from the interaction between genetic and environmental factors. The principal risk factors are obesity and age. Other relevant risk factors are craniofacial abnormalities, hypothyroidism and menopause, as well as the use of alcohol and sedatives. By virtue of its association with factors such as HLA levels, obesity, genetic syndromes, ethnicity, excessive sleepiness, alterations in ventilatory control and expression of inflammatory mediators, OSAS has been related to heritability. This chapter addresses the genetic and phenotypic variability of the disease, showing its relevance in the understanding of the physiopathology and clinical evaluation of OSAS.

  16. Rapid mass movement of chloroplasts during segment formation of the calcifying siphonalean green alga, Halimeda macroloba

    DEFF Research Database (Denmark)

    Larkum, Anthony W D; Salih, Anya; Kühl, Michael

    2011-01-01

    The calcifying siphonalean green alga, Halimeda macroloba is abundant on coral reefs and is important in the production of calcium carbonate sediments. The process by which new green segments are formed over-night is revealed here for the first time.......The calcifying siphonalean green alga, Halimeda macroloba is abundant on coral reefs and is important in the production of calcium carbonate sediments. The process by which new green segments are formed over-night is revealed here for the first time....

  17. Energy Balance of the Sao Paulo State -1998 Base year 1997; Balanco energetico do Estado de Sao Paulo - 1998. Ano base 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-01

    This energy balance of the Sao Paulo State presents the following main topics that can be outstanding: panorama of the energy matrix; supply and demand of energy by source 1983-1997; energy consumption by sector 1983/1997; economic aspects; resources and reserves energy; and balance of the transformation centers 1983/1997.

  18. Distribution and origin of hydrocarbons in water and sediment in Sao Sebastiao, SP, Brazil

    International Nuclear Information System (INIS)

    Zanardi, Eliete; Bicego, Marcia Caruso; Miranda, Luiz Bruner de; Weber, Rolf Roland

    1999-01-01

    This study describes the dissolved/dispersed petroleum hydrocarbons (DDPH) in surface water and the origin and distribution of aliphatic and aromatic hydrocarbons in samples from the surface bottom sediments. The main objective was to verify the anthropogenic contribution on the adjacent inner shelf of Sao Sebastiao, taking into account the distribution of hydrographic properties and knowledge of the main circulation. The DDPH concentration range of the 20 samples were from 0.35 to 2.50 m u g . L -1 , characterising this region as slightly affected by the contribution of petroleum hydrocarbons. Considering sediment hydrocarbon results, sites located in the inner shelf were divided into three groups: one with mainly biogenic hydrocarbons, a second one with biogenic and petroleum hydrocarbons and a third with significant contribution of petroleum hydrocarbons. These results show an influence of human activities in the Sao Sebastiao Channel on the surrounding areas of the inner shelf. (Author)

  19. Increased uptake of sup(99m)Tc-MDP in calcified synovial sarcoma

    International Nuclear Information System (INIS)

    Horne, T.; Mogle, P.; Finsterbush, A.; Gordin, M.; Hadassah Univ. Hospital, Mount Scopus; Hadassah Univ. Hospital, Mount Scopus

    1983-01-01

    We present a case of a partially calcified synovial sarcoma of the soft tissues of the thigh in a young girl. The roentgenographic, arteriographic and radio-nuclide scans were unusual. The finding and possible causes of increased uptake of sup(99m)Tc-MDP in synovial sarcoma are discussed. (orig.)

  20. Cell Phenotype Transitions in Cardiovascular Calcification

    Directory of Open Access Journals (Sweden)

    Luis Hortells

    2018-03-01

    Full Text Available Cardiovascular calcification was originally considered a passive, degenerative process, however with the advance of cellular and molecular biology techniques it is now appreciated that ectopic calcification is an active biological process. Vascular calcification is the most common form of ectopic calcification, and aging as well as specific disease states such as atherosclerosis, diabetes, and genetic mutations, exhibit this pathology. In the vessels and valves, endothelial cells, smooth muscle cells, and fibroblast-like cells contribute to the formation of extracellular calcified nodules. Research suggests that these vascular cells undergo a phenotypic switch whereby they acquire osteoblast-like characteristics, however the mechanisms driving the early aspects of these cell transitions are not fully understood. Osteoblasts are true bone-forming cells and differentiate from their pluripotent precursor, the mesenchymal stem cell (MSC; vascular cells that acquire the ability to calcify share aspects of the transcriptional programs exhibited by MSCs differentiating into osteoblasts. What is unknown is whether a fully-differentiated vascular cell directly acquires the ability to calcify by the upregulation of osteogenic genes or, whether these vascular cells first de-differentiate into an MSC-like state before obtaining a “second hit” that induces them to re-differentiate down an osteogenic lineage. Addressing these questions will enable progress in preventative and regenerative medicine strategies to combat vascular calcification pathologies. In this review, we will summarize what is known about the phenotypic switching of vascular endothelial, smooth muscle, and valvular cells.

  1. Tomographic appearance of calcified intervertebral discs in miniature Dachshund; Aparência tomográfica dos discos intervertebrais mineralizados em cães da raça Dachshund Miniatura

    Energy Technology Data Exchange (ETDEWEB)

    Baraúna Júnior, D.; Fernandes, T.H.T.; Silva, I.C.C.; Bonelli, M.A.; Santos, C.R.O.; Amorim, M.M.A., E-mail: durvalbarauna@hotmail.com [Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, PE (Brazil); Costa, F.S.; Tudury, E.A. [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil)

    2017-07-01

    The objective was to describe CT imaging of calcified IVD in one-year-old Dachshunds, as well as describe the location in the soft tissue structures that make up the IVD. Ten one-year-old dogs underwent computed tomography (CT). In sagittal reconstruction images of animals, the identification of 70 IVD present in the spaces between L3 - T9 were performed. Among calcified IVDs, the presence of the change was determined as the location in the nucleus pulposus (NP), annulus fibrous (AF) and both. Of a total of 70 evaluated IVDs, 45 were found to calcified and 25 not calcified. Among calcified discs, location was determined in nine (20%) in NP, eight discs (17.77%) in AF and 28 (62, 23%) NP and AF. Calcification in this study was characterized by increased radiographic attenuation mineral density in the IVD topography. There is variation in the location and radio density of calcified areas between animals and IVD in the same individual. Changes related to IVD calcification can be classified as tomographic location in AF, NP and NP and AF. (author)

  2. Anaerobic biodegradation of pentachlorophenol in a fixed-film reactor inoculated with polluted sediment from Santos-Sao Vicente Estuary, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Saia, F.T.; Damianovic, M.H.R.Z.; Cattony, E.B.M.; Brucha, G.; Foresti, E. [Sao Paulo Univ., Sao Carlos (Brazil). Lab. of Biological Processes; Vazoller, R.F. [Sao Paulo Univ., Sao Paulo (Brazil). Lab. of Environmental Microbiology

    2007-06-15

    This paper discusses the results of pentachlorophenol (PCP) anaerobic biodegradation in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor operated under methanogenic and halophylic conditions. The system was inoculated with autochthonous microorganisms taken from a site in the Santos-Sao Vicente Estuary (state of Sao Paulo, Brazil) severely contaminated with PCP, phenolic compounds, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, and heavy metals. The inoculum was previously enriched for methanogenesis activity by changing glucose concentrations and under halophylic condition. PCP was added to the HAIB reactor as sodium salt (NaPCP) at an initial concentration of 5 mg l{sup -1} and increased to 13, 15, and 21 mg l{sup -1}. Organic matter removal efficiency ranged from 77 to 100%. PCP removal efficiency was 100%. Denaturing gradient gel electrophoresis profile showed changes in the structure of Bacteria domain, which was associated with NaPCP and glucose amendments. The diversity of Archaea remained unaltered during the different phases. Scanning electron microscope examinations showed that cells morphologically resembling Methanosarcina and Methanosaeta predominated in the biofilm. These cells were detected by fluorescence in situ hybridization with the Methanosarcinales (MSMX860) specific probe. The results are of great importance in planning the estuary's restoration by using anaerobic technology and autochthonous microorganisms for bioremediation. (orig.)

  3. Metals in soils adjacent to avenues of highly dense traffic of Sao Paulo city, Brazil

    International Nuclear Information System (INIS)

    Ticianelli, Regina B.; Ribeiro, Andreza P.; Figueiredo, Ana M.G.; Nammoura-Neto, Georges M.; Silva, Nathalia C.

    2009-01-01

    Sao Paulo is the largest city in Brazil with about 19 millions of inhabitants in the metropolitan area, more than 8 million motor vehicles and strong industrial activity at the metropolitan region, which are responsible for increasing pollution in the region. Nevertheless, there is little information on metal contents in the metropolitan region soils, which would be very useful as a fingerprint of the environmental pollution. The present study aimed to determine As, Ba, Co, Cr, Sb and Zn concentrations in soils adjacent to avenues of highly dense traffic downtown Sao Paulo city: Consolacao/Reboucas Avenues; 23 de Maio Avenue and Tiradentes Avenue, to assess their possible sources and potential environmental impact. The analytical technique employed was Instrumental Neutron Activation Analysis (INAA). The results show metal concentration levels higher than the values reference values for soils of Sao Paulo, according to the Environmental Protection Agency of the State Sao Paulo (CETESB) guidelines. As, Ba and Zn showed concentration levels above the Intervention Values in some points, indicating direct or indirect potential risks to human health. The traffic related element Ba, Sb and Zn presented concentrations above the Prevention Values in points with high density traffic and may be associated to vehicular emissions. (author)

  4. Síndrome da apneia obstrutiva do sono (Saos e sonolência diurna excessiva (SDE: influência sobre os riscos e eventos de queda em idosos

    Directory of Open Access Journals (Sweden)

    Renata Afonso Burgos

    Full Text Available INTRODUÇÃO: Muitos países vêm experimentando o processo de envelhecimento populacional e a consequente elevação das doenças associadas a ele, como dificuldade de manter o equilíbrio, perdas na qualidade do sono e síndrome da apneia obstrutiva do sono (Saos. OBJETIVOS:Investigar a correlação entre a Saos e sonolência diurna excessiva (SDE com os riscos e eventos de quedas em indivíduos idosos. MATERIAIS E MÉTODOS:Estudo descritivo, comparativo, de corte transversal com amostra de 75 indivíduos com idade igual ou superior a 60 anos, gêneros masculino e feminino. Foram utilizados o mini-exame do estado mental; escalas de depressão geriátrica simplificada; de sonolência de epworth; de avaliação do equilíbrio de tinneti; índice de massa corporal (IMC; registros estabilométricos das oscilações posturais ântero-posterior (AP e médio-lateral (ML. RESULTADOS: Maior prevalência de Saos no gênero masculino. Não foi encontrada correlação com significância estatística (Pearson, p ≤ 0,01 entre as variáveis IMC e estabilometria. Não houve correlação estatisticamente significativa (ANOVA, p ≤ 0,05 entre IMC (subgrupos normal, sobrepeso, graus I, II, III, e IV e estabilometria; entre os graus de severidade de Saos e estabilometria; entre dados estabilométricos de subgrupos de IMC e mesmo grau de severidade de Saos; entre dados estabilométricos de subgrupos de IMC e diferentes graus de Saos; entre os diferentes graus de Saos (GC, G1, (GC e G2, subgrupos de IMC e registros estabilométricos. CONCLUSÃO: Não foram encontrados resultados que corroborassem a hipótese de proporcionalidade entre graus de severidade de Saos, IMC e registro estabilométrico.

  5. Early and late cretaceous magmatism from Sao Sebastiao island (SE-Brazil): geochemistry and petrology

    International Nuclear Information System (INIS)

    Bellieni, G.; Cavazzini, G.; Montes-Lauar, C.R.; Melfi, A.J.; Pacca, I.G.; De Min, A.; Piccirillo, E.M.

    1990-01-01

    The Sao Sebastiao island (236 km 2 ), located along the coast of the Sao Paulo State (Southern Brazil), is characterized by precambrian granitic affected by the Brasiliano tectonic-metamorphic cycle. This crystalline basement is intruded by Early Cretaceous (EC) sub alkaline basic and acid dykes, as well as by Late Cretaceous (LC) alkaline stocks (syenites) and dykes (basanite to phonolite). Geochemical, Sr-isotopic and mineral chemistry data point out that: EC-dykes reveal a basic-acid bimodal character, similar to that of the 'coeval' Parana basin flood volcanics; the acid dykes correspond, in composition, to the acid volcanics of the northern Parana basin: the EC-dykes can represent the eastern extension of the inland Santos-Rio de Janeiro dyke swarm, and LC alkaline stocks and dykes constitute distinct groups, characterized by different Sr-isotope initial ratios (syenites: av. 0.7052 and basanites + tephrites = av. 0.7045), which indicate that they are related to different time-integrated mantle source materials. (author)

  6. Distribution and origin of hydrocarbons in water and sediment in Sao Sebastiao, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Zanardi, Eliete [Universidade de Sao Paulo, Instituto Oceanografico, Sao Paulo, SP (Brazil); Bicego, Marcia Caruso; Miranda, Luiz Bruner de; Weber, Rolf Roland [Miami Univ., RSMAS/NAC, Miami, FL (United States)

    1999-04-01

    This study describes the dissolved/dispersed petroleum hydrocarbons (DDPH) in surface water and the origin and distribution of aliphatic and aromatic hydrocarbons in samples from the surface bottom sediments. The main objective was to verify the anthropogenic contribution on the adjacent inner shelf of Sao Sebastiao, taking into account the distribution of hydrographic properties and knowledge of the main circulation. The DDPH concentration range of the 20 samples were from 0.35 to 2.50 {sup m}u{sup g}. L{sup -1}, characterising this region as slightly affected by the contribution of petroleum hydrocarbons. Considering sediment hydrocarbon results, sites located in the inner shelf were divided into three groups: one with mainly biogenic hydrocarbons, a second one with biogenic and petroleum hydrocarbons and a third with significant contribution of petroleum hydrocarbons. These results show an influence of human activities in the Sao Sebastiao Channel on the surrounding areas of the inner shelf. (Author)

  7. Agriculture, Rio Sao Francisco, Brazil, South America

    Science.gov (United States)

    1988-01-01

    The large field patterns in this view of the Rio Sao Francisco basin, Brazil, South America, (11.5S, 43.5W) indicate a commercial agriculture venture; family subsistence farms are much smaller and laid out in different patterns. Land clearing in Brazil has increased at an alarming rate in recent years and preliminary estimates suggest a 25 to 30% increase in deforestation since 1984. The long term impact on the ecological processes are still unknown.

  8. An advanced automation system for operation of Sao Paulo pumping stations from TRANSPETRO Master Control Center - CNCO

    Energy Technology Data Exchange (ETDEWEB)

    Corcioli, Mario Sergio; Barreto, Camila Maria Benevenuto [TRANSPETRO, Rio de Janeiro, RJ (Brazil)

    2009-07-01

    Since 2000 the operations of the TRANSPETRO pumping stations in the state of Sao Paulo region began to be transferred from local control centers located at the transfer and storage terminals to the National Operational Control Center (CNCO) of TRANSPETRO, located at the headquarters of the company, in Rio de Janeiro. The proposed paper aims to presenting an overview of the automation system that was developed to enable such pumping stations to be operated from CNCO in a reliable and secure manner, focusing on tools that offer an embedded system alarms completely free of false alarms with automatic determining of the root cause, and also automatic and advanced diagnoses of problems caused by failures of hardware, human error and abnormal conditions of the process, providing the CNCO SCADA system of accurate and quality information that help operators to make decisions. The referred automation system was integrated for the first time to CNCO SCADA system in 2000, for pipeline pumps of Osvat (Sao Sebastiao - Vale do Paraiba pipeline) station at the Sao Sebastiao Terminal - northern coast of Sao Paulo region. (author)

  9. Potential of municipal solid waste (MSW) as a source of energy in Sao Paulo: its impact on CO2 balance

    International Nuclear Information System (INIS)

    Leao, A.L.; Ing Hwie Tan

    1998-01-01

    Energy generation is needed in Sao Paulo and MSW represents a promising alternative, although it is more expensive than hydroelectric power. About 14 900 t/day of MSW is generated, of which 8433 t/day is domestic and commercial MSW. From this amount, 1800 t will be destined to generate 30 MW of power. The eco-balance of CO 2 has been considered for incineration and recycling. The recycling program of plastics, metals, paper and glass would represent a significant reduction in energy and CO 2 emission. The total CO 2 released is 3.34 x 10 5 t/yr without recycling, and is 1.25 x 10 5 t/yr with a recycling program. Most of the CO 2 comes from plastics and paper production. Economic aspects could probably favor incineration with energy production as the best option. (author)

  10. Atmospheric pollution biomonitoring of the Sao Paulo metropolitan region using epiphytic lichens

    International Nuclear Information System (INIS)

    Fuga, Alessandra

    2006-01-01

    Due to the increasing problems of atmospheric pollution in the Sao Paulo metropolitan region that affect the environment and human health the application of biomonitoring methodologies using cosmopolite organisms has now become relevant. Biomonitoring is a method to evaluate the response of live organisms to pollution. This method offers advantages such as reduced costs, efficient monitoring of large geographic areas and accumulated pollutants over a large period in which low concentrations of chemicals elements in the environment can be evaluated. In the present study, neutron activation analysis method was applied to determine elements accumulated in Canoparmelia texana lichenized fungi. Samples were collected in two distinct areas: Carlos Botelho (PECB) and Intervales (PEI) State Parks that are considered as non-polluted areas and that belong to the Atlantic Forest - SP ecosystem; and Sao Paulo city metropolitan region in sites near automatic monitoring stations of the Environmental Protection Agency of the State of Sao Paulo (CETESB). The lichens collected from the bark of the trees were properly treated, and irradiated with neutrons from IEA-R1 nuclear reactor along with synthetic standards of elements. The precision and the accuracy of the results were evaluated by the analyses of IAEA-336 LICHEN and Mixed Polish Herbs (INCT -MPH-2) certified reference materials. The results obtained for these materials were in accordance with the certified values and presented good precision with variation coefficients ranging from 0.9 to 14.6%. Results obtained for lichens showed that elements As, Co, Cr, Cs, La, Mo, Sb, Sc, Se and U are present at ng g -1 levels, Ba, Br, Cl, Fe, K, Mn, Na, Rb and Zn at μg g -1 and Ca at mg g -1 . By applying cluster and discriminant analyses to the results for the lichen samples from areas with different levels of pollution, the sampling sites were grouped according to their chemical similarities and their elemental composition. It was

  11. Investigating the provenance of iron artifacts of the Royal Iron Factory of Sao Joao de Ipanema by hierarchical cluster analysis of EDS microanalyses of slag inclusions

    Energy Technology Data Exchange (ETDEWEB)

    Mamani-Calcina, Elmer Antonio; Landgraf, Fernando Jose Gomes; Azevedo, Cesar Roberto de Farias, E-mail: c.azevedo@usp.br [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Escola Politecnica. Departmento de Engenharia Metalurgica e de Materiais

    2017-01-15

    Microstructural characterization techniques, including EDX (Energy Dispersive X-ray Analysis) microanalyses, were used to investigate the slag inclusions in the microstructure of ferrous artifacts of the Royal Iron Factory of Sao Joao de Ipanema (first steel plant of Brazil, XIX century), the D. Pedro II Bridge (located in Bahia, assembled in XIX century and produced in Scotland) and the archaeological sites of Sao Miguel de Missoes (Rio Grande do Sul, Brazil, production site of iron artifacts, the XVIII century) and Afonso Sardinha (Sao Paulo, Brazil production site of iron artifacts, XVI century). The microanalyses results of the main micro constituents of the microstructure of the slag inclusions were investigated by hierarchical cluster analysis and the dendrogram with the microanalyses results of the wüstite phase (using as critical variables the contents of MnO, MgO, Al{sub 2}O{sub 3}, V{sub 2}O{sub 5} and TiO{sub 2}) allowed the identification of four clusters, which successfully represented the samples of the four investigated sites (Ipanema, Sardinha, Missoes and Bahia). Finally, the comparatively low volumetric fraction of slag inclusions in the samples of Ipanema (∼1%) suggested the existence of technological expertise at the iron making processing in the Royal Iron Factory of Sao Joao de Ipanema. (author)

  12. Report of the First International Consensus on Standardized Nomenclature of Antinuclear Antibody HEp-2 Cell Patterns (ICAP) 2014-2015

    OpenAIRE

    Chan, Edward K L; Damoiseaux, Jan; Carballo, Orlando Gabriel; Conrad, Karsten; de Melo Cruvinel, Wilson; Francescantonio, Paulo Luiz Carvalho; Fritzler, Marvin J.; Garcia-De La Torre, Ignacio; Herold, Manfred; Mimori, Tsuneyo; Satoh, Minoru; von Mühlen, Carlos A.; Andrade, Luis E C

    2015-01-01

    During the 12th International Workshop on Autoantibodies and Autoimmunity held in Sao Paulo, Brazil, on August 28, 2014, a full day session was devoted to establishing a consensus on the nomenclature of staining patterns observed in the antinuclear antibody (ANA) indirect immunofluorescence test on HEp-2 cells. The current report summarizes the collective agreements with input from the host Brazilian and international communities that represented research, clinical, and diagnostic service lab...

  13. U, TH and lanthanides in street soils of Sao Paulo city, Brazil

    International Nuclear Information System (INIS)

    Ticianelli, R.B.; Ribeiro, A.P.; Figueiredo, A.M.G.; Zanh, G.S.

    2013-01-01

    The study of lanthanide distribution in urban environments has become of interest over the last years, due to the increased industrial use of these elements. Sao Paulo is the 6th largest metropolitan region of the world, with about 20 million inhabitants in its metropolitan area, more than 9 million motor vehicles and intense industrial activity. There is little information on U, Th, and lanthanide contents in urban soils, and there are as of yet no reference values for these elements in soils of Sao Paulo city. The present study aimed to determine U, Th and lanthanide concentrations in soils adjacent to avenues of highly dense traffic downtown in Sao Paulo city, to assess their possible sources and potential environmental impacts. The analytical technique employed was Instrumental Neutron Activation Analysis (INAA). Th and U levels ranged from 4.0 to 37.0 mg kg -1 and from 1.6 to 18.7 mg kg -1 , respectively. These values are higher than literature values for U and Th in Brazilian superficial soils. The results obtained for the lanthanides indicate enrichment in La and Ce. However, a possible anthropogenic source should be investigated since high background values of these elements may be associated to the natural geological composition of the soils. (author)

  14. Demand of radiopharmaceutical Fluoride 18-FDG (fluorodeoxyglucose) in the Sao Paulo State metropolitan area

    International Nuclear Information System (INIS)

    Sato, Renato C.; Zouain, Desiree M.

    2005-01-01

    This research presents partial results from the development of a Masters Dissertation for the Post-Graduation in Nuclear Technology Program - IPEN/USP, aiming to study the demand of radiopharmaceutical Fluoride 18-FDG (fluorodeoxyglucose) in the Sao Paulo State metropolitan area, as a subsidiary for the establishment of distribution strategy within the State. This study presented the results of a bibliographic review as well as the market evolution for FDG in Sao Paulo. Studies pointed to a tendency of an increase in the international and national nuclear medicine market; while the United States of America participate in 47% of the world profit, South America shares only 2.5% of the global market. This market will tend to grow in 2006 to 2020 up to 776% for diagnosis and 760% for therapy. Partial results are presented in this study from researching medical centers that use PET in the city of Sao Paulo, as well as companies that commercialize the equipment and the manufacturer center. There is an increase of sales for IPEN's Fluoride 18-FDG and its representation on the total radiopharmaceutical profit surpassed 5.3% in 2003 to 8.2% in 2004. The dissemination of this technology in Brazil is lately being discussed especially due to the acquisition price of the equipment as well as the viability of the resources (Fluoride 18- FDG; implementation strategies of regional cyclotron accelerators) and the question of remuneration of the PET produced exams for health care plans and national health care system (SUS). IPEN is developing yet another study to grasp possible demand for this product in the Southern and Southeastern regions, allowing better view of the necessity of the supplement, and in study the implementation of a new cyclotron in the institute dedicated for the production of Fluoride 18-FDG. (author)

  15. Dual-energy CT head bone and hard plaque removal for quantification of calcified carotid stenosis: utility and comparison with digital subtraction angiography

    International Nuclear Information System (INIS)

    Uotani, Kensuke; Watanabe, Yoshiyuki; Higashi, Masahiro; Nakazawa, Tetsuro; Kono, Atsushi K.; Hori, Yoshiro; Fukuda, Tetsuya; Kanzaki, Suzu; Yamada, Naoaki; Naito, Hiroaki; Itoh, Toshihide; Sugimura, Kazuro

    2009-01-01

    We evaluated quantification of calcified carotid stenosis by dual-energy (DE) CTA and dual-energy head bone and hard plaque removal (DE hard plaque removal) and compared the results to those of digital subtraction angiography (DSA). Eighteen vessels (13 patients) with densely calcified carotid stenosis were examined by dual-source CT in the dual-energy mode (tube voltages 140 kV and 80 kV). Head bone and hard plaques were removed from the dual-energy images by using commercial software. Carotid stenosis was quantified according to NASCET criteria on MIP images and DSA images at the same plane. Correlation between DE CTA and DSA was determined by cross tabulation. Accuracies for stenosis detection and grading were calculated. Stenosis could be evaluated in all vessels by DE CTA after applying DE hard plaque removal. In contrast, conventional CTA failed to show stenosis in 13 out of 18 vessels due to overlapping hard plaque. Good correlation between DE plaque removal images and DSA images was observed (r 2 =0.9504) for stenosis grading. Sensitivity and specificity to detect hemodynamically relevant (>70%) stenosis was 100% and 92%, respectively. Dual-energy head bone and hard plaque removal is a promising tool for the evaluation of densely calcified carotid stenosis. (orig.)

  16. Location study of thermoelectric in Sao Paulo's State, Brazil

    International Nuclear Information System (INIS)

    Fadigas, Eliane Aparecida F.A.; Reis, Lineu Belico dos

    1999-01-01

    The article presents the results of studies developed to identify, locate and order thermoelectric generation options through costs increasing. This evaluation considered just options of Sao Paulo, a Brazilian state. The used methodology and the main parameters and variables which influence in the projects economic activity are introduced in the article. A software denominated SAEGET was also used

  17. Environmental radioactive monitoring in Itu, Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    1990-01-01

    The results of the environmental monitoring of a region near to a radioactive materials deposit in Sao Paulo State, Brazil, are presented. The radioactive materials are uranium and thorium hydroxides from monazite processing. The temporal variation of 226 Ra was determined in the superficial and underground water, showing no increase for the former and a maximum concentration of 0,306 Bq/L for the latter. 21 figs., 17 tabs

  18. Three-year results after directional atherectomy of calcified stenotic lesions of the superficial femoral artery.

    Science.gov (United States)

    Minko, P; Buecker, A; Jaeger, S; Katoh, M

    2014-10-01

    To investigate the 3-year outcome of patients with peripheral arterial disease (PAD) and heavily calcified stenotic lesions of the superficial femoral artery after directional atherectomy. Fifty-three patients (mean age 67 ± 10 years; 18 females, 35 males, TASC B and C, mean lesion length 7.9 ± 3.5 cm) with PAD (Rutherford 2-6) were enrolled into this prospective monocentric study. In total, 59 calcified lesions of the superficial femoral artery were treated with the Silverhawk atherectomy device (Covidien, Plymouth, MN, USA). Patients were followed-up for 36 months with a 6-month interval to perform clinical re-evaluation, including measurement of maximum walking distance and ankle-brachial index (ABI) as well as duplex-sonography. The primary success rate of the procedure was 92 %. In five cases (8 %), additional balloon-PTA and/or stent-PTA was necessary. Procedure-related embolization occurred in seven cases (12 %), which were all successfully treated by aspiration. The primary patency rate after 3 years was 55 %. Median Rutherford score decreased significantly from 5 to 0 after 36 months (p atherectomy was successfully applied to decrease the plaque burden. Results after 3 years showed a significant decrease of Rutherford score with persistent improvement of ABI and reasonable patency rate.

  19. Prevalence and populations of Listeria monocytogenes in meat products retailed in Sao Paulo, Brazil.

    Science.gov (United States)

    Ristori, Christiane Asturiano; Rowlands, Ruth Estela Gravato; Martins, Cecília Geraldes; Barbosa, Maria Luisa; Yoshida, Júlia T U; Franco, Bernadette D G de Melo

    2014-12-01

    This study evaluated the prevalence of the populations and serotypes of Listeria monocytogenes in 552 refrigerated samples of ground beef, chicken leg, hot dog, and pork sausage collected in supermarkets in the city of Sao Paulo, SP, Brazil, between May 2008 and July 2009. The supermarkets were selected after stratification by geographical region and by random draw. Tests for presence and enumeration of L. monocytogenes were based on ISO 11290-1:1996/Amd.1:2004 and ISO 11290-2:1998 methods, respectively. Listeria spp. were detected in 469 (85.0%) of the studied meat products. The most frequently isolated species was L. innocua (64.1%), followed by L. monocytogenes (48.7%), L. welshimeri (13.4%), L. seeligeri (7.1%), L. ivanovii (0.2%), and L. grayi subspecies murrayi (0.2%). L. monocytogenes was detected in 269 (48.7%) samples, with highest prevalence in ground beef (59.4%) followed by chicken legs (58.0%), pork sausages (39.8%), and hot dogs (37.7%). The populations were Prevalence of serotypes varied according to the type of meat product. These data are relevant for estimating the risks of listeriosis associated with consumption of meat products in Sao Paulo, and for establishing science-based intervention strategies aimed at reducing these risks, especially for pregnant women and immunocompromised individuals.

  20. Calcifying epithelial odontogenic tumor, a rare presentation in children: Two case reports

    Directory of Open Access Journals (Sweden)

    Susant Mohanty

    2014-01-01

    Full Text Available Calcifying epithelial odontogenic tumor (CEOT is a rare and benign odontogenic neoplasm that affects the jaws. It is certainly an atypical instance to find this tumor in children. Here, we present two case reports of CEOT presenting in mandible of a 12- and 13-year-old female child, respectively. CEOT have been reported to show features of malignant transformation also.

  1. GiSAO.db: a database for ageing research

    Directory of Open Access Journals (Sweden)

    Grillari Johannes

    2011-05-01

    Full Text Available Abstract Background Age-related gene expression patterns of Homo sapiens as well as of model organisms such as Mus musculus, Saccharomyces cerevisiae, Caenorhabditis elegans and Drosophila melanogaster are a basis for understanding the genetic mechanisms of ageing. For an effective analysis and interpretation of expression profiles it is necessary to store and manage huge amounts of data in an organized way, so that these data can be accessed and processed easily. Description GiSAO.db (Genes involved in senescence, apoptosis and oxidative stress database is a web-based database system for storing and retrieving ageing-related experimental data. Expression data of genes and miRNAs, annotation data like gene identifiers and GO terms, orthologs data and data of follow-up experiments are stored in the database. A user-friendly web application provides access to the stored data. KEGG pathways were incorporated and links to external databases augment the information in GiSAO.db. Search functions facilitate retrieval of data which can also be exported for further processing. Conclusions We have developed a centralized database that is very well suited for the management of data for ageing research. The database can be accessed at https://gisao.genome.tugraz.at and all the stored data can be viewed with a guest account.

  2. Determination of natural radioactivity in public water supplies of Sao Paulo State. V.1-2

    International Nuclear Information System (INIS)

    Oliveira, Joselene de

    1998-01-01

    This study aimed to determine the activity concentrations of natural occurring radionuclides of the 238 U and 232 Th series in drinking water supplies of Sao Paulo State. A sampling program was carried out, in order to perform this survey, starting in 1994 and covering 54% of the 574 existing counties. The locations studied correspond to the public water systems operated routinely by SABESP - Companhia de Saneamento Basico do Estado de Sao Paulo, which is the state company responsible for collecting, treating and supplying water. The activity concentrations of 226 Ra and 228 Ra were determined by gross alpha and beta counting, respectively, of a Ba(Ra)SO 4 precipitate in a low-background gasflow proportional counter. The 228 Rn concentrations were determined by liquid scintillation method. Concentrations up to 235 mBq/L and 131 mBq/L were observed for 226 Rn and 228 Ra, respectively, whereas 222 Rn concentrations reached 315 Bq/L. Of the 452 water samples analyzed, a total of 3 presented activity concentrations for 226 Ra above the limit of 0.1 Bq/L adopted by the Brazilian regulation for the gross-alpha activity in drinking water. These results, however, are below 1/50 of the annual limit of intake recommended for 226 Ra by 'Comissao Nacional de Energia Nuclear', if an ingestion rate of 2L/d per person is considered. For the gross-beta activity, all the measured samples presented 228 Ra activity concentrations below 1 Bq/L. It is concluded that the consumption of such waters will not imply in any additional health risk to the population. In order to evaluate the radioactive doses due to the ingestion of these waters, a conservative dosimetric calculation was carried out using dose conversion factors suggested by ICRP (International Commission on Radiological Protection). Doses up to 0.3 mSv/y, 0.6 mSv/y and 3.2 mSv/y were estimated for the critical organs, for the ingestion of 226 Ra, 228 Ra and 222 Rn, respectively; while the corresponding committed effective

  3. Melan-A/Mart-1- or HMB-45-positive melanocytes are not present in calcifying cystic odontogenic tumors (calcifying odontogenic cysts): a study in 13 Caucasian patients.

    Science.gov (United States)

    Tosios, Konstantinos I; Prountzos, Nikolaos; Katsoulas, Nikolaos; Koutlas, Ioannis G; Sklavounou-Andrikopoulou, Alexandra

    2012-03-01

    Melanin pigment and melanocytes may be found in odontogenic cysts and tumors, particularly calcifying cystic odontogenic tumor (CCOT). In the present study we investigated the immunohistochemical expression of the Melan-A/Mart-1 and HMB-45 antigens in 13 Caucasians patients with CCOT. Melan-A/Mart-1- and HMB-45-positive melanocytes were not seen in any of the cases. Our findings are in agreement with the assumption that pigmentation in odontogenic lesions may be a racial phenomenon.

  4. Sao Paulo Lightning Mapping Array (SP-LMA): Deployment and Plans

    Science.gov (United States)

    Bailey, J. C.; Carey, L. D.; Blakeslee, R. J.; Albrecht, R.; Morales, C. A.; Pinto, O., Jr.

    2011-01-01

    An 8-10 station Lightning Mapping Array (LMA) network is being deployed in the vicinity of Sao Paulo to create the SP-LMA for total lightning measurements in association with the international CHUVA [Cloud processes of tHe main precipitation systems in Brazil: A contribUtion to cloud resolVing modeling and to the GPM (GlobAl Precipitation Measurement)] field campaign. Besides supporting CHUVA science/mission objectives and the Sao Luz Paraitinga intensive operation period (IOP) in December 2011-January 2012, the SP-LMA will support the generation of unique proxy data for the Geostationary Lightning Mapper (GLM) and Advanced Baseline Imager (ABI), both sensors on the NOAA Geostationary Operational Environmental Satellite-R (GOES-R), presently under development and scheduled for a 2015 launch. The proxy data will be used to develop and validate operational algorithms so that they will be ready for use on "day1" following the launch of GOES-R. A preliminary survey of potential sites in the vicinity of Sao Paulo was conducted in December 2009 and January 2010, followed up by a detailed survey in July 2010, with initial network deployment scheduled for October 2010. However, due to a delay in the Sa Luz Paraitinga IOP, the SP-LMA will now be installed in July 2011 and operated for one year. Spacing between stations is on the order of 15-30 km, with the network "diameter" being on the order of 30-40 km, which provides good 3-D lightning mapping 150 km from the network center. Optionally, 1-3 additional stations may be deployed in the vicinity of Sa Jos dos Campos.

  5. Largest case series of Latin American eyelid tumors over 13-Years from a single center in Sao Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Jéssica Carolinne Damasceno

    Full Text Available ABSTRACT Purpose: Malignant lesions of the eyelid are the most common eye cancers. Although rarely lethal, late diagnosis can lead to serious visual morbidity. Definitive diagnosis of neoplastic lesions is pathological. Indications and types of eyelid lesions in a single high-volume ophthalmic reference center in Sao Paulo, Brazil, were analyzed. Methods: The surgical pathological reports of patients who underwent eye removal procedures between January 2000 and December 2012 were retrieved from the electronic database of the Ophthalmology Department and retrospectively reviewed. Data regarding the final anatomopathological diagnosis, sex, and age were analyzed via the χ2 test with Yates' correction. Results: Of the 1,113 eyelid tumors resected over the 13-year study period, 324 (29% lesions were malignant. The most prevalent lesions were basal cell, squamous cell, and sebaceous gland carcinomas. The median ages of patients (females, n=165, 51%; males, n=159, 49% with a diagnosis of basal cell, squamous cell, and sebaceous gland carcinomas were 65, 75, and 70 years, respectively. Conclusion: This is the largest retrospective cohort analysis of eyelid tumors in a Latin American population. These findings are in agreement with those from large centers in other countries and regions.

  6. High-energy extracorporeal shock-wave therapy for calcifying tendinitis of the rotator cuff: a randomised trial.

    Science.gov (United States)

    Albert, J-D; Meadeb, J; Guggenbuhl, P; Marin, F; Benkalfate, T; Thomazeau, H; Chalès, G

    2007-03-01

    In a prospective randomised trial of calcifying tendinitis of the rotator cuff, we compared the efficacy of dual treatment sessions delivering 2500 extracorporeal shock waves at either high- or low-energy, via an electromagnetic generator under fluoroscopic guidance. Patients were eligible for the study if they had more than a three-month history of calcifying tendinitis of the rotator cuff, with calcification measuring 10 mm or more in maximum dimension. The primary outcome measure was the change in the Constant and Murley Score. A total of 80 patients were enrolled (40 in each group), and were re-evaluated at a mean of 110 (41 to 255) days after treatment when the increase in Constant and Murley score was significantly greater (t-test, p = 0.026) in the high-energy treatment group than in the low-energy group. The improvement from the baseline level was significant in the high-energy group, with a mean gain of 12.5 (-20.7 to 47.5) points (p energy group. Total or subtotal resorption of the calcification occurred in six patients (15%) in the high-energy group and in two patients (5%) in the low-energy group. High-energy shock-wave therapy significantly improves symptoms in refractory calcifying tendinitis of the shoulder after three months of follow-up, but the calcific deposit remains unchanged in size in the majority of patients.

  7. Associations of coronary artery calcified plaque density with mortality in type 2 diabetes: the Diabetes Heart Study.

    Science.gov (United States)

    Raffield, Laura M; Cox, Amanda J; Criqui, Michael H; Hsu, Fang-Chi; Terry, James G; Xu, Jianzhao; Freedman, Barry I; Carr, J Jeffrey; Bowden, Donald W

    2018-05-11

    Coronary artery calcified plaque (CAC) is strongly predictive of cardiovascular disease (CVD) events and mortality, both in general populations and individuals with type 2 diabetes at high risk for CVD. CAC is typically reported as an Agatston score, which is weighted for increased plaque density. However, the role of CAC density in CVD risk prediction, independently and with CAC volume, remains unclear. We examined the role of CAC density in individuals with type 2 diabetes from the family-based Diabetes Heart Study and the African American-Diabetes Heart Study. CAC density was calculated as mass divided by volume, and associations with incident all-cause and CVD mortality [median follow-up 10.2 years European Americans (n = 902, n = 286 deceased), 5.2 years African Americans (n = 552, n = 93 deceased)] were examined using Cox proportional hazards models, independently and in models adjusted for CAC volume. In European Americans, CAC density, like Agatston score and volume, was consistently associated with increased risk of all-cause and CVD mortality (p ≤ 0.002) in models adjusted for age, sex, statin use, total cholesterol, HDL, systolic blood pressure, high blood pressure medication use, and current smoking. However, these associations were no longer significant when models were additionally adjusted for CAC volume. CAC density was not significantly associated with mortality, either alone or adjusted for CAC volume, in African Americans. CAC density is not associated with mortality independent from CAC volume in European Americans and African Americans with type 2 diabetes.

  8. Pb-210 deposition measured in rainfall in Sao Paulo, SP-Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Damatto, Sandra R.; Frujuele, Jonatan V.; Souza, Joseilton M.; Santos, Levi F., E-mail: damatto@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Lab. de Radiometria Ambiental

    2013-07-01

    Pb-210 (T{sub 1/2} = 22.3 y), a natural radionuclide from U-238 serie can be found in the atmosphere, as a product of {sup 222}Rn decay that emanates from the ground, where its atoms become rapidly fixed to aerosols and return to the earth as dry fallout or are washed out in the rain. This natural radionuclide has been widely used as an atmospheric tracer, to determine the aerosol residence time as well as chronometers in the environment. Pb-210 was measured during a period of two years, 2011 to 2013, in samples of rainfall in all the rainy events that occurred at the Instituto de Pesquisas Energeticas e Nucleares (IPEN) campus (23 deg 33’59.24” S - 46 deg 44’15.63” O at 760 m above sea level) which is located in the city of Sao Paulo, in the state of Sao Paulo, Brazil. Pb-210 concentration was measured in a total of 123 rainy events by beta gross counting in a low background gas flow proportional detector, after radiochemistry procedure. The results obtained were correlated to seasons and rainfall. The concentrations of {sup 210}Pb in rainfall varied from the minimum detectable activity, 4.9 mBq L{sup -1} to 1408± 43 mBq L{sup -1}. The highest concentrations were obtained in the months of winter and the lowest in summer. The monthly depositional flux of {sup 210}Pb, varied from 4.03 Bq m{sup -2} month{sup -1} to 46.4 Bq m{sup -2} month{sup -1}presenting a strong correlation with the amount of precipitation and hence showing seasonal trends. (author)

  9. The potential cost-effectiveness of the Diamondback 360® Coronary Orbital Atherectomy System for treating de novo, severely calcified coronary lesions: an economic modeling approach.

    Science.gov (United States)

    Chambers, Jeffrey; Généreux, Philippe; Lee, Arthur; Lewin, Jack; Young, Christopher; Crittendon, Janna; Mann, Marita; Garrison, Louis P

    2016-04-01

    Patients who undergo percutaneous coronary intervention (PCI) for severely calcified coronary lesions have long been known to have worse clinical and economic outcomes than patients with no or mildly calcified lesions. We sought to assess the likely cost-effectiveness of using the Diamondback 360(®) Orbital Atherectomy System (OAS) in the treatment of de novo, severely calcified lesions from a health-system perspective. In the absence of a head-to-head trial and long-term follow up, cost-effectiveness was based on a modeled synthesis of clinical and economic data. A cost-effectiveness model was used to project the likely economic impact. To estimate the net cost impact, the cost of using the OAS technology in elderly (⩾ 65 years) Medicare patients with de novo severely calcified lesions was compared with cost offsets. Elderly OAS patients from the ORBIT II trial (Evaluate the Safety and Efficacy of OAS in Treating Severely Calcified Coronary Lesions) [ClinicalTrials.gov identifier: NCT01092426] were indirectly compared with similar patients using observational data. For the index procedure, the comparison was with Medicare data, and for both revascularization and cardiac death in the following year, the comparison was with a pooled analysis of the Harmonizing Outcomes with Revascularization and Stents in Acute Myocardial Infarction (HORIZONS-AMI)/Acute Catheterization and Urgent Intervention Triage Strategy (ACUITY) trials. After adjusting for differences in age, gender, and comorbidities, the ORBIT II mean index procedure costs were 17% (p economic modeling, the recently approved coronary OAS device is projected to be highly cost-effective for patients who undergo PCI for severely calcified lesions. © The Author(s), 2015.

  10. Synthesis, characterization and anti cancer activity of some fluorinated 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles

    Directory of Open Access Journals (Sweden)

    Deepak Chowrasia

    2017-05-01

    Full Text Available A series of fluorinated 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles (2a–2i was synthesized by condensation of various substituted 4-amino-5-phenyl-4H-1,2,4-triazole-3-thiols (1a–1i with penta fluoro benzoic acid in good yields (60–80%. The synthesized compounds were screened for anticancer activity against three cancerous cell lines MCF7 (human breast cancer, SaOS-2 (human osteosarcoma and K562 (human myeloid leukemia. The compounds showed moderate to good antiproliferative potency against the studied cell lines. Among these, compound 2b showed higher antiproliferative activity (IC50 22.1, 19 and 15 μM against MCF7, SaOS-2 and K562, respectively while 2a exhibited least antiproliferative activity (IC50 30.2, 39 and 29.4 μM against MCF7, SaOS-2 and K562 cells, respectively. Therefore, the present study demonstrates that fluorine substituted 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles would be a better prospective in the development of anticancer drugs.

  11. A rare fatality due to calcified stylohyoid ligament (Eagle syndrome).

    Science.gov (United States)

    Gupta, Avneesh; Aggrawal, Anil; Setia, Puneet

    2017-06-01

    The elongation or calcification of the stylohyoid ligament that leads to pressure symptoms, or entrapment of nearby glossopharyngeal nerve or carotid artery, is known as Eagle syndrome. A PubMed search leads to finding of rare fatality among the 49 reported cases. In the present case, the deceased was a 40-year-old male who choked on his food. We hypothesise that the impaction of food in the upper respiratory tract, as well as the inability to intubate the person, were both the result of the calcified stylohyoid ligament.

  12. Metal and trace element assessment of sediments from Guarapiranga reservoir, Sao Paulo State, by neutron activation analysis

    International Nuclear Information System (INIS)

    Guimaraes, Guilherme M.; Favaro, Deborah I.T.; Franklin, Robson L.; Ferreira, Francisco J.; Bevilacqua, Jose E.

    2009-01-01

    Guarapiranga Reservoir is extremely important due to the fact that it is one of the main water reservoirs for South America's largest city, Sao Paulo, Brazil. Guarapiranga Basin is located within the Metropolitan Region of Sao Paulo - RMSP, and occupies an area of approximately 630 km 2 , and the reservoir itself is located in the northern part of the basin occupying approximately 26 km 2 . This reservoir is characterized by environmental impacts from urban invasion, industrial and sewage wastes, all of which seriously affect its water quality. Due to its vulnerability CETESB (Environmental Control Agency of the Sao Paulo State) regularly monitors the contamination levels of waters and once a year sediment samples. In order to better understand geochemical and environmental processes and their possible changes due to anthropogenic activities trace metals analyses and their distribution in sediments are commonly undertaken. The present study reports results concerning the distribution of some major (Fe, K and Na), trace (As, Ba, Br, Co, Cr, Cs, Hf, Hg, Rb, Sb, Sc, Ta, Tb, Th, U and Zn) and rare earth (Ce, Eu, La, Lu, Nd, Sm, Tb and Yb) elements in sediments from the Guarapiranga Reservoir. Multielementar analysis was carried out by instrumental neutron activation analysis (INAA). Multielemental concentrations in the sediment samples were compared to NASC (North American Shale Composite) values. The concentration values for metals As, Cr and Zn in the sediment samples were compared to the Canadian Council of Minister of the Environment (CCME) oriented values (TEL and PEL values) and adopted by CETESB. (author)

  13. Computed tomographic diagnosis of calcified inferior vena cava thrombus in a child with Wilm's tumor

    Energy Technology Data Exchange (ETDEWEB)

    Kirks, D R; Ponzi, J W; Korobkin, M

    1980-01-01

    A calcified thrombus in the inferior vena cava of infants and children may be imaged by computed tomography. The precise location of the calcification within the inferior vena cava may be confirmed by computed tomographic scanning during injection of intravenous contrast material.

  14. Biomineralisation with Saos-2 bone cells on TiSiN sputtered Ti alloys.

    Science.gov (United States)

    V V, Anusha Thampi; Bendavid, Avi; Martin, P J; Vaithilingam, Vijay; Bean, Penelope A; Evans, Margaret D M; Subramanian, B

    2017-07-01

    Surface modifications of metallic implants are important in order to protect the underlying metals from the harsh corrosive environment inside the human body and to minimize the losses caused by wear. Recently, researches are carried out in developing bioactive surfaces on metallic implants, which supports the growth and proliferation of cells on to these surfaces. Titanium silicon nitride (TiSiN) hard nanocomposites thin films were fabricated on Ti alloys (Ti-6Al-4V) by pulsed direct current (DC) reactive magnetron sputtering. The films were characterized for its microstructural and electrochemical behavior. The higher charge transfer resistance (Rct) and positive shift in Ecorr value of TiSiN/Ti alloys than the bare Ti-alloys indicates a better corrosion resistance offered by the TiSiN thin films to the underlying substrates. The biological response to TiSiN/Ti alloys and control bare Ti-alloys was measured in vitro using cell-based assays with two main outcomes. Firstly, neither the Ti alloy nor the TiSiN thin film was cytotoxic to cells. Secondly, the TiSiN thin film promoted differentiation of human bone cells above the bare control Ti alloy as measured by alkaline phosphatase and calcium production. TiSiN thin films provide better corrosion resistance and protect the underlying metal from the corrosive environment. The thin film surface is both biocompatible and bioactive as indicated from the cytotoxicity and biomineralization studies. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Vanglaülestõus vallandas Sao Paulos kaose / Erik Henno

    Index Scriptorium Estoniae

    Henno, Erik

    2006-01-01

    11. mail vallandus Sao Paulo vanglates konflikt, mis laienes üle osariigi ning mille käigus süüdati kümneid busse, rünnati politseiautosid ja pangakontoreid, pantvangi võeti ligi 200 inimest. Kaubanduskeskused ja koolid jäid suletuks, ühistransport oli halvatud. Kaart: Vägivallalaine Brasiilias. Lisa: Maffia

  16. Opposite cytokine synthesis by fibroblasts in contact co-culture with osteosarcoma cells compared with transwell co-cultures.

    Science.gov (United States)

    David, Manu S; Kelly, Elizabeth; Zoellner, Hans

    2013-04-01

    We recently reported exchange of membrane and cytoplasm during contact co-culture between human Gingival Fibroblasts (h-GF) and SAOS-2 osteosarcoma cells, a process we termed 'cellular sipping' to reflect the manner in which cells become morphologically diverse through uptake of material from the opposing cell type, independent of genetic change. Cellular sipping is increased by Tumor Necrosis Factor-α (TNF-α), and we here show for the first time altered cytokine synthesis in contact co-culture supporting cellular sipping compared with co-culture where h-GF and SAOS-2 were separated in transwells. SAOS-2 had often undetectably low cytokine levels, while Interleukin-6 (IL-6), Granulocyte Colony Stimulating Factor (G-CSF) and Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) were secreted primarily by TNF-α stimulated h-GF and basic Fibroblast Growth Factor (FGF) was prominent in h-GF lysates (p cultures permitting cellular sipping had lower IL-6, G-CSF and GM-CSF levels, as well as higher lysate FGF levels compared with TNF-α treated h-GF alone (p cultures in transwells, with increased IL-6, G-CSF and GM-CSF levels (p cultures where cellular sipping occurs, potentially contributing to tumor inflammatory responses. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

  17. An Einstein Observatory SAO-based catalog of B-type stars

    Science.gov (United States)

    Grillo, F.; Sciortino, S.; Micela, G.; Vaiana, G. S.; Harnden, F. R., Jr.

    1992-01-01

    About 4000 X-ray images obtained with the Einstein Observatory are used to measure the 0.16-4.0 keV emission from 1545 B-type SAO stars falling in the about 10 percent of the sky surveyed with the IPC. Seventy-four detected X-ray sources with B-type stars are identified, and it is estimated that no more than 15 can be misidentified. Upper limits to the X-ray emission of the remaining stars are presented. In addition to summarizing the X-ray measurements and giving other relevant optical data, the present extensive catalog discusses the reduction process and analyzes selection effects associated with both SAO catalog completeness and IPC target selection procedures. It is concluded that X-ray emission, at the level of Lx not less than 10 exp 30 ergs/s, is quite common in B stars of early spectral types (B0-B3), regardless of luminosity class, but that emission, at the same level, becomes less common, or nonexistent, in later B-type stars.

  18. Conjugation of a cell-penetrating peptide to parathyroid hormone affects its structure, potency, and transepithelial permeation

    DEFF Research Database (Denmark)

    Kristensen, Mie; de Groot, Anne Marit; Berthelsen, Jens

    2015-01-01

    hormone, i.e. PTH(1-34), and to evaluate the effect with regards to secondary structure, potency in Saos-2 cells, immunogenicity, safety as well as the transepithelial permeation across monolayers by using the Caco-2 cell culture model. Further, co-administration of CPP and PTH(1-34) as an alternative...

  19. Hospitalization flow in the public and private systems in the state of Sao Paulo, Brazil.

    Science.gov (United States)

    Rocha, Juan Stuardo Yazlle; Monteiro, Rosane Aparecida; Moreira, Marizélia Leão

    2015-01-01

    OBJECTIVE To describe the migration flows of demand for public and private hospital care among the health regions of the state of Sao Paulo, Brazil.METHODS Study based on a database of hospitalizations in the public and private systems of the state of Sao Paulo, Southeastern Brazil, in 2006. We analyzed data from 17 health regions of the state, considering people hospitalized in their own health region and those who migrated outwards (emigration) or came from other regions (immigration). The index of migration effectiveness of patients from both systems was estimated. The coverage (hospitalization coefficient) was analyzed in relation to the number of inpatient beds per population and the indexes of migration effectiveness.RESULTS The index of migration effectiveness applied to the hospital care demand flow allowed characterizing health regions with flow balance, with high emigration of public and private patients, and with high attraction of public and private patients.CONCLUSIONS There are differences in hospital care access and opportunities among health regions in the state of Sao Paulo, Brazil.

  20. [Urban and rural population of the state of Sao Paulo: results of the census of 1980].

    Science.gov (United States)

    Saad, P M

    1981-01-01

    The accelerated urban growth of Sao Paulo between 1940-70 has continued during the period 1970-80, according to the 1980 censes. During 1970-80 the urban population increased 55.47%, while the rural population decreased 18.67%, bringing the percentage of the urban population to 88.6% of the total population of the state. This phenomenon has been common to all the 11 administrative regions of the state. The highest percentage of the urban population in 1980 was in the region of Greater Sao Paulo, followed by Litoral and Vale do Paraiba. The largest increases in urban population were in the regions of Sorocaba, Campinas, and Vale do Paraiba, while the highest decreases in rural population were in the regions of Sao Jose do Rio Preto, Aracatuba, Presidente Prudente, and Marilia. The document presents detailed data for each of the 11 administrative regions of the state, and for each municipality within a region.

  1. "Unusual brain stone": heavily calcified primary neoplasm with some features suggestive of angiocentric glioma.

    Science.gov (United States)

    Sajjad, Jahangir; Kaliaperumal, Chandrasekaran; Bermingham, Niamh; Marks, Charles; Keohane, Catherine

    2015-11-01

    This 40-year-old man presented with a 5-month history of progressive right-sided headache associated with visual blurring. He also had a history of epilepsy but had been seizure free with medication for the past 10 years. An initial CT scan of his brain performed 16 years previously had revealed a small area of calcification in the right parietal region. In the current presentation, he had a left-sided homonymous hemianopia but no other neurological deficits. A CT scan of his brain showed a much larger calcified, partly cystic lesion in the right parietal region. Because he was symptomatic, the lesion was excised and the cyst was drained. Histological examination of the excised tissue showed an unusual primary tumor that was difficult to classify but had some features of angiocentric glioma. The heavy calcification, mixed-density cell population, and regions with features of angiocentric glioma were most unusual. The patient remained asymptomatic 5 years after surgery, and follow-up scans did not show recurrence.

  2. Comparison of the diagnostic accuracy of FBP, ASiR, and MBIR reconstruction during CT angiography in the evaluation of a vessel phantom with calcified stenosis in a distal superficial femoral artery in a cadaver extremity.

    Science.gov (United States)

    Tsukada, Jitsuro; Yamada, Minoru; Yamada, Yoshitake; Yamazaki, Shun; Imanishi, Nobuaki; Tamura, Kentaro; Hashimoto, Masahiro; Nakatsuka, Seishi; Jinzaki, Masahiro

    2016-07-01

    To investigate whether adaptive statistical iterative reconstruction (ASiR) or model-based iterative reconstruction (MBIR) improves the diagnostic performance of computed tomography angiography (CTA) for small-vessel calcified lesions relative to filtered back projection (FBP) using cadaver extremities and a calcified stenosis phantom. A cadaver was used in accordance with our institutional regulations, and a calcified stenosis phantom simulating 4 grades of stenosis was prepared. The phantom was inserted within the distal superficial femoral artery of the cadaver leg. Ten CT images per reconstruction type and stenosis grade were acquired using a 64-slice multidetector-row CTA.As an objective measurement, the first and second derivatives of the CT value function profiles were calculated. As a subjective measurement, 2 blinded reviewers measured the stenosis ratio using a quantitative scale. The Wilcoxon rank-sum test was used to evaluate the data. Objective measurements of both 25% and 50% stenosis differed significantly (P ASiR and FBP.Reviewer 2's subjective measurements of 25% stenosis differed significantly (P ASiR (40.89 ± 3.14%), and the measurements of 50% stenosis differed significantly (P ASiR and FBP. MBIR improves the diagnostic performance of CTA for small-vessel calcified lesions relative to FBP.

  3. p53-dependent and p53-independent anticancer activity of a new indole derivative in human osteosarcoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Cappadone, C., E-mail: concettina.cappadone@unibo.it [Department of Pharmacy and Biotechnology, University of Bologna, Bologna (Italy); Stefanelli, C. [Department for Life Quality Studies, University of Bologna, Rimini Campus, Rimini (Italy); Malucelli, E. [Department of Pharmacy and Biotechnology, University of Bologna, Bologna (Italy); Zini, M. [Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna (Italy); Onofrillo, C. [Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna (Italy); Locatelli, A.; Rambaldi, M.; Sargenti, A. [Department of Pharmacy and Biotechnology, University of Bologna, Bologna (Italy); Merolle, L. [ELETTRA–Sincrotrone Trieste S.C.p.A., Trieste (Italy); Farruggia, G. [Department of Pharmacy and Biotechnology, University of Bologna, Bologna (Italy); National Institute of Biostructures and Biosystems, Roma (Italy); Graziadio, A. [Department of Pharmacy and Biotechnology, University of Bologna, Bologna (Italy); Montanaro, L. [Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna (Italy); Iotti, S. [Department of Pharmacy and Biotechnology, University of Bologna, Bologna (Italy); National Institute of Biostructures and Biosystems, Roma (Italy)

    2015-11-13

    Osteosarcoma (OS) is the most common primary malignant tumor of bone, occurring most frequently in children and adolescents. The mechanism of formation and development of OS have been studied for a long time. Tumor suppressor pathway governed by p53 gene are known to be involved in the pathogenesis of osteosarcoma. Moreover, loss of wild-type p53 activity is thought to be a major predictor of failure to respond to chemotherapy in various human cancers. In previous studies, we described the activity of a new indole derivative, NSC743420, belonging to the tubulin inhibitors family, capable to induce apoptosis and arrest of the cell cycle in the G2/M phase of various cancer cell lines. However, this molecule has never been tested on OS cell line. Here we address the activity of NSC743420 by examine whether differences in the p53 status could influence its effects on cell proliferation and death of OS cells. In particular, we compared the effect of the tested molecule on p53-wild type and p53-silenced U2OS cells, and on SaOS2 cell line, which is null for p53. Our results demonstrated that NSC743420 reduces OS cell proliferation by p53-dependent and p53-independent mechanisms. In particular, the molecule induces proliferative arrest that culminate to apoptosis in SaOS2 p53-null cells, while it brings a cytostatic and differentiating effect in U2OS cells, characterized by the cell cycle arrest in G0/G1 phase and increased alkaline phosphatase activity. - Highlights: • The indole derivative NSC743420 induces antitumor effects on osteosarcoma cells. • p53 status could drive the activity of antitumor agents on osteosarcoma cells. • NSC743420 induces cytostatic and differentiating effects on U2OS cells. • NSC743420 causes apoptosis on p53-null SaOS2 cells.

  4. p53-dependent and p53-independent anticancer activity of a new indole derivative in human osteosarcoma cells

    International Nuclear Information System (INIS)

    Cappadone, C.; Stefanelli, C.; Malucelli, E.; Zini, M.; Onofrillo, C.; Locatelli, A.; Rambaldi, M.; Sargenti, A.; Merolle, L.; Farruggia, G.; Graziadio, A.; Montanaro, L.; Iotti, S.

    2015-01-01

    Osteosarcoma (OS) is the most common primary malignant tumor of bone, occurring most frequently in children and adolescents. The mechanism of formation and development of OS have been studied for a long time. Tumor suppressor pathway governed by p53 gene are known to be involved in the pathogenesis of osteosarcoma. Moreover, loss of wild-type p53 activity is thought to be a major predictor of failure to respond to chemotherapy in various human cancers. In previous studies, we described the activity of a new indole derivative, NSC743420, belonging to the tubulin inhibitors family, capable to induce apoptosis and arrest of the cell cycle in the G2/M phase of various cancer cell lines. However, this molecule has never been tested on OS cell line. Here we address the activity of NSC743420 by examine whether differences in the p53 status could influence its effects on cell proliferation and death of OS cells. In particular, we compared the effect of the tested molecule on p53-wild type and p53-silenced U2OS cells, and on SaOS2 cell line, which is null for p53. Our results demonstrated that NSC743420 reduces OS cell proliferation by p53-dependent and p53-independent mechanisms. In particular, the molecule induces proliferative arrest that culminate to apoptosis in SaOS2 p53-null cells, while it brings a cytostatic and differentiating effect in U2OS cells, characterized by the cell cycle arrest in G0/G1 phase and increased alkaline phosphatase activity. - Highlights: • The indole derivative NSC743420 induces antitumor effects on osteosarcoma cells. • p53 status could drive the activity of antitumor agents on osteosarcoma cells. • NSC743420 induces cytostatic and differentiating effects on U2OS cells. • NSC743420 causes apoptosis on p53-null SaOS2 cells.

  5. Apoptosis may determine the release of skeletal alkaline phosphatase activity from human osteoblast-line cells.

    Science.gov (United States)

    Farley, J R; Stilt-Coffing, B

    2001-01-01

    Although quantitative measurement of skeletal alkaline phosphatase (sALP) activity in serum can provide an index of the rate of bone formation, the metabolic process that determines the release of sALP - from the surface of osteoblasts, into circulation-is unknown. The current studies were intended to examine the hypothesis that the release of sALP from human osteoblasts is a consequence of apoptotic cell death. We measured the release of sALP activity from human osteosarcoma (SaOS-2) cells and normal human bone cells, under basal conditions and in response to agents that increased apoptosis (TNF-a, okadiac acid) and agents that inhibit apoptosis (IGF-I, calpain, and caspase inhibitors). Apoptosis was determined by the presence of nucleosomes (histone-associated DNA) in the cytoplasm of the cells by using a commercial kit. The results of these studies showed that TNF-a and okadiac acid caused dose- and time-dependent increases in apoptosis in the SaOS-2 cells (r = 0.78 for doses of TNF-a and r = 0.93 for doses of okadiac acid, P sALP activity (e.g., r = 0.89 for TNF-a and r = 0.75 for okadiac acid, P sALP activity (P sALP activity (P sALP release. The associations between apoptosis and sALP release were not unique to osteosarcoma (i.e., SaOS-2) cells, but also seen with osteoblast-line cells derived from normal human bone. Together, these data demonstrate that the release of sALP activity from human osteoblast-line cells in vitro is associated with, and may be a consequence of, apoptotic cell death. These findings are consistent with the general hypothesis that the appearance of sALP activity in serum may reflect the turnover of osteoblast-line cells.

  6. Determination of Cl, K, and Na in the blood serum of healthy elderly in the city of Sao Paulo, SP; Determinacao de Cl, K e Na em soro sanguineo de idosos saudaveis da cidade de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Nathalia Souza de; Saiki, Mitiko, E-mail: natsouzaraujo@yahoo.com.b, E-mail: mitiko@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2009-07-01

    This work determined the blood serum concentration of Cl, K, and Na from a healthy elderly population of the city of Sao Paulo, Sao Paulo, Brazil viewing the comparison with reference values used in clinical laboratories. For collecting these samples the present work were approved by the Ethic Committee of the FMUSP and IPEN-CNEN/SP. The quality of the analytical results were evaluated by the analysis of the reference material certification NIST 1577b Bovine Liver. The results presented good accuracy and precision due to the fact that they presented relative errors less than 5.2 % and standard deviation less than 8.6 %. The average concentrations (mmol L{sup -1}) of Cl in the analysed serum were 99.1; the K were 3.9 and the Na were 136.9. The obtained data allows the to conclude that the mostly of the elderly presented Cl, K and Na concentrations in the range of reference values used in the clinical laboratories

  7. Energy balance of Sao Paulo state, Brazil 2008. Calendar year 2007; Balanco energetico do Estado de Sao Paulo 2008. Ano base 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    This document presents the consolidated annual energy balances and also tables and graphics which detailed the evolution and the present status of offer and demand of each energy resources used by Sao Paulo, Brazil, economy and regionalized and environmental information. It covers the period of 1994 to 2007 and includes energy fluxes of the energy primary and secondary resources, from the production to the final consumption of the main sectors of the state economy.

  8. Heterogeneity of osteosarcoma cell lines led to variable responses in reprogramming.

    Science.gov (United States)

    Choong, Pei Feng; Teh, Hui Xin; Teoh, Hoon Koon; Ong, Han Kiat; Choo, Kong Bung; Sugii, Shigeki; Cheong, Soon Keng; Kamarul, Tunku

    2014-01-01

    Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity of the reprogrammed sarcomas showed all four reprogrammed sarcoma lines could differentiate into adipocytes while reprogrammed Saos-2-REP, MG-63-REP and G-292-REP could differentiate into osteocytes. Among the 4 osteosarcoma cell lines, U-2 OS reported the highest transduction efficiency but recorded the lowest reprogramming stability under long term culture. Thus, there may be intrinsic differences governing the variable responses of osteosarcoma cell lines towards reprogramming and long term culture effect of the reprogrammed cells. This is a first report to associate intrinsic factors in different osteosarcoma cell lines with variable reprogramming responses and effects on the reprogrammed cells after prolonged culture.

  9. Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming

    Science.gov (United States)

    Choong, Pei Feng; Teh, Hui Xin; Teoh, Hoon Koon; Ong, Han Kiat; Choo, Kong Bung; Sugii, Shigeki; Cheong, Soon Keng; Kamarul, Tunku

    2014-01-01

    Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity of the reprogrammed sarcomas showed all four reprogrammed sarcoma lines could differentiate into adipocytes while reprogrammed Saos-2-REP, MG-63-REP and G-292-REP could differentiate into osteocytes. Among the 4 osteosarcoma cell lines, U-2 OS reported the highest transduction efficiency but recorded the lowest reprogramming stability under long term culture. Thus, there may be intrinsic differences governing the variable responses of osteosarcoma cell lines towards reprogramming and long term culture effect of the reprogrammed cells. This is a first report to associate intrinsic factors in different osteosarcoma cell lines with variable reprogramming responses and effects on the reprogrammed cells after prolonged culture. PMID:25170299

  10. Prevalence, Predictors, and Clinical Presentation of a Calcified Nodule as Assessed by Optical Coherence Tomography.

    Science.gov (United States)

    Lee, Tetsumin; Mintz, Gary S; Matsumura, Mitsuaki; Zhang, Wenbin; Cao, Yang; Usui, Eisuke; Kanaji, Yoshihisa; Murai, Tadashi; Yonetsu, Taishi; Kakuta, Tsunekazu; Maehara, Akiko

    2017-08-01

    This study sought to determine the anatomic characteristics and clinical presentation associated with a calcified nodule (CN) as assessed by optical coherence tomography. CN is an unusual but demonstrable cause of acute coronary syndromes (ACS). We studied 889 de novo culprit lesions in 889 patients (48% ACS) who underwent optical coherence tomography before intervention. CN was defined as an eruptive accumulation of nodular calcification (small fractured calcifications). Using quantitative coronary angiography, the change in the angle of the lesion between diastole and systole was measured (angiographic Δ angle). CN was seen in 4.2% of all lesions and was located more frequently in the ostial or mid right coronary artery. Hemodialysis (odds ratio: 4.0; 95% confidence interval: 1.1 to 13.4; p = 0.04), in-lesion angiographic Δ angle (odds ratio: 1.09; 95% confidence interval: 1.05 to 1.14; p presentation, there was a smaller minimum lumen area (1.04 mm 2 [first quartile, third quartile: 0.69, 1.26] vs. 1.61 [first quartile, third quartile: 1.03, 2.06] mm 2 ; p = 0.02) accompanied by more thrombus (82.4% vs. 20.0%; p presentation. In lesions with severe calcification (maximum calcium arc >180°), 30% of ACS culprit lesions contained a CN, and the presence of a CN was associated with ACS presentation independent of other vulnerable plaque morphologies. The presence of a CN was associated with severe calcification and larger hinge movement of the coronary artery (especially ostial and mid right coronary artery). One-third of the underlying plaque morphology of severely calcified culprit lesions in patients with ACS was caused by a CN. Copyright © 2017 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.

  11. Effect of Ocean Acidification on the Food Quality of the Coccolithophore Emiliania huxleyi

    Science.gov (United States)

    Maine, J. E.; White, M. M.; Balch, W. M.; Milke, L. M.

    2016-02-01

    The anthropogenic burning of fossil fuels has doubled atmospheric carbon dioxide (CO2) levels over the last 200 years. Atmospheric CO2 diffuses into the ocean, changing the chemistry and decreasing the pH of seawater in a process called Ocean Acidification (OA). Calcifying marine phytoplankton, coccolithophores, are vulnerable to OA. Emiliania huxleyi is a lipid-dense and globally-abundant species of coccolithophore, therefore it is a vital food source for higher marine trophic levels. The objective of this project was to determine how OA affects the lipid profile and calcification of E. huxleyi CCMP #371. Gas chromatography was used to determine how the proportions of saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA) in E. huxleyi varied with increasing pCO2. Flow cytometry was used to measure how the distribution of highly calcified cells, partially calcified cells, and un-calcified cells changed with increasing pCO2. The proportion of MUFA increased with pCO2. The proportion of un-calcified and partially calcified cells increased with increasing pCO2, however, the results varied across two experimental runs. In conclusion, the lipid-profile and calcification properties of E. huxleyi, and likely its food quality to predators, are affected by OA.

  12. Astronomical observations with the optical multichannel analyser of the Sao Paulo University

    Energy Technology Data Exchange (ETDEWEB)

    Codina-Landaberry, S J; de Freitas Pacheco, J A [Universidade Mackenzie, Sao Paulo (Brazil). Centro de Radio-Astronomia e Astrofisica

    1979-03-01

    The first astronomical observations made with the optical multichannel analyser (OMA) of the Sao Paulo University is reported. A detailed description of the instrumentation is given as well as the results derived from the observation of some emission nebulae.

  13. Calcified carotid atherosclerotic plaques on digital panoramic radiographs in patients with Type II diabetes mellitus: A case control study

    Directory of Open Access Journals (Sweden)

    Neha Khambete

    2015-01-01

    Full Text Available Aim: Diabetes mellitus is associated with accelerated carotid artery atherosclerosis and increased risk of stroke. This study was conducted with the objective of determining the prevalence of calcified atherosclerotic plaques on panoramic radiographs of patients with Type II diabetes mellitus. Materials and Methods: Panoramic radiographs of 100 patients (age range 50-84 years with known history of type II diabetes mellitus, visiting the outpatient department were evaluated for the presence of calcified atherosclerotic plaques. Age- and sex-matched controls were evaluated in the same manner. Statistical comparison of prevalence rates was done. Results: The radiographs of diabetics (mean age: 64.45 years revealed that 26% had atheromatous plaques, whereas those of controls (mean age: 65.36 years revealed that 6% had atheromatous plaques. A statistically significant difference (P = 0.01410 was obtained using Yates′ Chi-square test. Conclusion: People with diabetes mellitus had a greater prevalence of calcified atherosclerotic plaques on panoramic radiographs than non-diabetics. Panoramic radiographs of diabetic patients should be screened for the presence of carotid artery atheromatous plaques for timely medical referral of asymptomatic patients and avoiding any further serious consequences like cerebrovascular accidents.

  14. Lung cells support osteosarcoma cell migration and survival.

    Science.gov (United States)

    Yu, Shibing; Fourman, Mitchell Stephen; Mahjoub, Adel; Mandell, Jonathan Brendan; Crasto, Jared Anthony; Greco, Nicholas Giuseppe; Weiss, Kurt Richard

    2017-01-25

    Osteosarcoma (OS) is the most common primary bone tumor, with a propensity to metastasize to the lungs. Five-year survival for metastatic OS is below 30%, and has not improved for several decades despite the introduction of multi-agent chemotherapy. Understanding OS cell migration to the lungs requires an evaluation of the lung microenvironment. Here we utilized an in vitro lung cell and OS cell co-culture model to explore the interactions between OS and lung cells, hypothesizing that lung cells would promote OS cell migration and survival. The impact of a novel anti-OS chemotherapy on OS migration and survival in the lung microenvironment was also examined. Three human OS cell lines (SJSA-1, Saos-2, U-2) and two human lung cell lines (HULEC-5a, MRC-5) were cultured according to American Type Culture Collection recommendations. Human lung cell lines were cultured in growth medium for 72 h to create conditioned media. OS proliferation was evaluated in lung co-culture and conditioned media microenvironment, with a murine fibroblast cell line (NIH-3 T3) in fresh growth medium as controls. Migration and invasion were measured using a real-time cell analysis system. Real-time PCR was utilized to probe for Aldehyde Dehydrogenase (ALDH1) expression. Osteosarcoma cells were also transduced with a lentivirus encoding for GFP to permit morphologic analysis with fluorescence microscopy. The anti-OS efficacy of Disulfiram, an ALDH-inhibitor previously shown to inhibit OS cell proliferation and metastasis in vitro, was evaluated in each microenvironment. Lung-cell conditioned medium promoted osteosarcoma cell migration, with a significantly higher attractive effect on all three osteosarcoma cell lines compared to basic growth medium, 10% serum containing medium, and NIH-3 T3 conditioned medium (p cell conditioned medium induced cell morphologic changes, as demonstrated with GFP-labeled cells. OS cells cultured in lung cell conditioned medium had increased alkaline

  15. Improved performance of the Sao Paulo Pelletron

    International Nuclear Information System (INIS)

    Sala, O.; Stier, M.; Acquadro, J.C.

    1981-01-01

    The Sao Paulo Pelletron was the first Pelletron constructed by the NEC and also the first to become operational at the end of 1971. Although the Herb Pelletron design was demonstrated from the beginning to be very good (later this was proven by the successful operation of several larger Pelletrons) our machine did suffer from defects in engineering which limited its performance and its application as a reliable tool for research in nuclear physics. This paper describes some of the improvements which we have introduced to increase the reliability of our Pelletron as a research tool in the University. (orig.)

  16. Astronomical observations with the optical multichannel analyser of the Sao Paulo University

    International Nuclear Information System (INIS)

    Codina-Landaberry, S.J.; Freitas Pacheco, J.A. de

    1979-01-01

    The first astronomical observations made with the optical multichannel analyser (OMA) of the Sao Paulo University is reported. A detailed description of the instrumentation is given as well as the results derived from the observation of some emission nebulae [pt

  17. Airport Choice in Sao Paulo Metropolitan Area: An Application of the Conditional Logit Model

    Science.gov (United States)

    Moreno, Marcelo Baena; Muller, Carlos

    2003-01-01

    Using the conditional LOGIT model, this paper addresses the airport choice in the Sao Paulo Metropolitan Area. In this region, Guarulhos International Airport (GRU) and Congonhas Airport (CGH) compete for passengers flying to several domestic destinations. The airport choice is believed to be a result of the tradeoff passengers perform considering airport access characteristics, airline level of service characteristics and passenger experience with the analyzed airports. It was found that access time to the airports better explain the airport choice than access distance, whereas direct flight frequencies gives better explanation to the airport choice than the indirect (connections and stops) and total (direct plus indirect) flight frequencies. Out of 15 tested variables, passenger experience with the analyzed airports was the variable that best explained the airport choice in the region. Model specifications considering 1, 2 or 3 variables were tested. The model specification most adjusted to the observed data considered access time, direct flight frequencies in the travel period (morning or afternoon peak) and passenger experience with the analyzed airports. The influence of these variables was therefore analyzed across market segments according to departure airport and flight duration criteria. The choice of GRU (located neighboring Sao Paulo city) is not well explained by the rationality of access time economy and the increase of the supply of direct flight frequencies, while the choice of CGH (located inside Sao Paulo city) is. Access time was found to be more important to passengers flying shorter distances while direct flight frequencies in the travel period were more significant to those flying longer distances. Keywords: Airport choice, Multiple airport region, Conditional LOGIT model, Access time, Flight frequencies, Passenger experience with the analyzed airports, Transportation planning

  18. Wind power system for Sao Gabriel, Irece region, Bahia-Brazil; Sistema eolico de Sao Gabriel, regiao de Irece-Bahia

    Energy Technology Data Exchange (ETDEWEB)

    Bruni, Carlos D' Alexandria [Centro Federal de Educacao Tecnologica da Bahia (CEFET-BA), Salvador, BA (Brazil)], e-mail: carlosbruni@cefetba.br; Camelier, Luiz Alberto A. [Companhia de Engenharia Rural da Bahia, Salvador, BA (Brazil)], e-mail: lcamelier@ig.com.br

    2004-07-01

    An electric power plant supplied by a wind-generator is the solution for supply drink water on a small community on Sao Gabriel, Irece - Bahia -Brazil. On behalf of the feasibility a wind power system is described in detail concerning it's technical specifications, it's operation, constraints and it's energy demand. Furthermore wind power system supply is described in detail. Simulations is shows how the system is designed to guarantee a reliability in pumping of drinking water powered by wind power system and it's expansion in the future. (author)

  19. Energy Balance of the Sao Paulo State -1998 Base year 1997

    International Nuclear Information System (INIS)

    1998-01-01

    This energy balance of the Sao Paulo State presents the following main topics that can be outstanding: panorama of the energy matrix; supply and demand of energy by source 1983-1997; energy consumption by sector 1983/1997; economic aspects; resources and reserves energy; and balance of the transformation centers 1983/1997

  20. The booster for the pelletron of Sao Paulo

    International Nuclear Information System (INIS)

    Acquadro, J.C.; Added, N.; Ferraretto, M.D.; Oliveira, O.; Ordonez, J.; Pessoa, E.; Sala, O.; Szanto de Toledo, A.

    1992-01-01

    This paper describes the necessary modifications in the present acceleration system for the coupling of a superconducting linac to the tandem of Sao Paulo. Final laboratory projectile energies of 15 MV/A to elements from C to Fe should be achieved after installing the two first cryostats (phase I). The superconducting linac will consist of split-ring niobium resonant cavities (F o = 97 MHz and beta = 0.0060, 0.105) that are being built in the Argonne National Laboratory. (Author) 4 refs., 3 figs

  1. New perspectives on rare connective tissue calcifying diseases.

    Science.gov (United States)

    Rashdan, Nabil A; Rutsch, Frank; Kempf, Hervé; Váradi, András; Lefthériotis, Georges; MacRae, Vicky E

    2016-06-01

    Connective tissue calcifying diseases (CTCs) are characterized by abnormal calcium deposition in connective tissues. CTCs are caused by multiple factors including chronic diseases (Type II diabetes mellitus, chronic kidney disease), the use of pharmaceuticals (e.g. warfarin, glucocorticoids) and inherited rare genetic diseases such as pseudoxanthoma elasticum (PXE), generalized arterial calcification in infancy (GACI) and Keutel syndrome (KTLS). This review explores our current knowledge of these rare inherited CTCs, and highlights the most promising avenues for pharmaceutical intervention. Advancing our understanding of rare inherited forms of CTC is not only essential for the development of therapeutic strategies for patients suffering from these diseases, but also fundamental to delineating the mechanisms underpinning acquired chronic forms of CTC. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  2. The Mesoproterozoic volcano-sedimentary Serra do Itaberaba Group of the Central Ribeira Belt, Sao Paulo State, Brazil: implications for the age of the overlying Sao Roque Group

    International Nuclear Information System (INIS)

    Juliani, Caetano; Hackspacher, Peter; Fetter, Allen Hutchenson; Dantas, Elton Luiz

    2000-01-01

    One of the fundamental problems to understanding the evolution of volcano-sedimentary sequences in southeastern Brazil is constraining their depositional ages. Brasiliano tectonic and metamorphic either obscured or destroyed primary features, such as unconformities, as well as other geologic relationships. This problem is exemplified by the Serra do Itaberaba and Sao Roque groups, where the lack of data about the timing of their deposition has prevented resolution of proposed one-and two-stage geotectonic/depositional models. Recent U-Pb zircon data obtained from metavolcanic rocks in the Sao Roque Group indicate that it was deposited between 628 and 607 Ma. New U-Pb zircon data of 1395± 10 Ma for a metandesite in the basal Morro da Pedra Preta Formation (Serra do Itaberaba Group) indicate the maximum age for the beginning of the deposition of the pelites overlying MORB-like basalt. A metarhyolite of the upper unit, the Nhangucu Formation, contains two zircon populations. One yielded an age of 619 ±3 Ma, which defines the crystallization age of the rock, and the other an age of 1449 ±3 Ma, interpreted as inherited xenocrystal grains from older units of the Serra do Itaberaba Group. The younger metarhyolite was affected only by the S 2 foliation, generated during the Brasiliano orogenesis, whereas the Middle Proterozoic metavolcano-sedimentary sequence records additional metamorphic and deformational events, confirming the presence of two different geotectonic cycles. (author)

  3. Annual report 2006 - CPFL Energy - Light and Power Company from Sao Paulo, SP

    International Nuclear Information System (INIS)

    2006-01-01

    This document reports the activities during 2006 of the Sao Paulo Light and Power (CPFL) as follows: society evolution, corporative and ethic management; economic and financial performance; operational performance, sustainability management and corporative responsibility

  4. A new energy control system for the Sao Paulo 8UD pelletron

    International Nuclear Information System (INIS)

    Acquadro, J.C.; Schnitter, U.; Teles, A.P.; Pereira, D.; Sala, O.; Freitas, P.A.B.

    1986-01-01

    The conventional control slits of the analyzing magnet have been substituted by a new energy control system using a thin carbon foil, for the 8UD Pelletron of the Universidade de Sao Paulo. This report describes the preliminary tests and results. (orig.)

  5. Carbon nanotube-coating accelerated cell adhesion and proliferation on poly (L-lactide)

    International Nuclear Information System (INIS)

    Hirata, Eri; Akasaka, Tsukasa; Uo, Motohiro; Takita, Hiroko; Watari, Fumio; Yokoyama, Atsuro

    2012-01-01

    Highlights: ► The surface of a polylactic acid (PLLA) was coated multiwalled carbon nanotubes (MWCNTs). ► MWCNT-coated PLLA showed remarkable higher wettability than uncoated PLLA. ► More Human osteosarcoma cell line (Saos2) adhered on the CNT-coated than those on uncoated PLLA at 2 h after seeding. ► MWCNT-coating on PLLA improved the surface wettability and initial cell attachment at early stage. - Abstract: The surface of a polylactic acid (PLLA) was coated multiwalled carbon nanotubes (MWCNTs) in order to improve the surface properties. In addition, its surface characteristics and cell culturing properties were examined. Whole surface of PLLA was homogeneously covered by MWCNTs maintained a unique tubular structure. MWCNT-coated PLLA showed remarkable higher wettability than uncoated PLLA. Human osteosarcoma cell line (Saos2) adhered well on the CNT-coated PLLA whereas there are few cells attached on the uncoated PLLA at 2 h after seeding. The number of the cells on uncoated PLLA was still smaller than on the MWCNT-coated PLLA at 1 and 3 days. Moreover, The DNA content in the cells attached to the MWCNT-coated PLLA was significantly higher than that on the uncoated PLLA (p 0.1). Therefore MWCNT-coating on PLLA improved the surface wettability and initial cell attachment at early stage.

  6. Quantifying impacts on air quality of vehicular emissions in Sao Paulo and Rio de Janeiro

    Science.gov (United States)

    Artaxo, Paulo; Ferreira de Brito, Joel; Godoy, José Marcus; Luiza Godoy, Maria; Junior, Djacinto

    2016-04-01

    Vehicular emissions in megacities such as Sao Paulo and Rio de Janeiro are increasingly becoming a global issue. The São Paulo Metropolitan Area (SPMA), located in Southeast of Brazil, is a megacity with a population of 18 million people, with 7 million cars and large-scale industrial emissions. Rio de Janeiro is also a large city with different meteorology than São Paulo. All cars in Brazil runs gasohol, with 23% ethanol in gasoline, and for the last 10 years, flex cars that can run on gasohol, ethanol or any mixture dominate the market. Overall ethanol accounts for about 30-40% of fuel burned in both cities. To improve the understanding of vehicular emission impacts on aerosol composition and life cycle in these two large megacities a source apportionment study, combining online and offline measurements, was performed. Aerosols were collected for one year to capture seasonal variability at 4 sites in each city, with inorganic and organic aerosol component being sampled. Organic and elemental carbon were measured using a Sunset Laboratory Dual Optics (transmission and reflectance) Carbon Analyzer and about 22 trace elements has been measured using polarized X-Ray Fluorescence (XRF). Aerosol mass and black carbon were also measured, as well as trace gases to help in aerosol source apportionment. In Sao Paulo, the average PM2.5 mass concentration obtained varied from 9.6 to 12.2 μg m-3 for the several sites, and similar concentrations were measured in Rio de Janeiro. At all sites, organic matter (OM) has dominated fine mode aerosol concentration with 42 to 60% of the aerosol mass. EC accounted for 21 to 31% of fine mode aerosol mass concentration. Sulfate accounted for 21 to 26% of PM2.5 for the sites. Aerosol source apportionment was done with receptor analysis and integration with online data such as PTR-MS, Aethalometers, Nephelometers and ACSM helped to apportion vehicular emissions. For the 8 sites operated in Sao Paulo and Rio de Janeiro, vehicular

  7. Fibroblast growth factor-2 induces osteogenic differentiation through a Runx2 activation in vascular smooth muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Nakahara, Takehiro; Sato, Hiroko; Shimizu, Takehisa; Tanaka, Toru; Matsui, Hiroki; Kawai-Kowase, Keiko; Sato, Mahito; Iso, Tatsuya; Arai, Masashi [Department of Medicine and Biological Science, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511 (Japan); Kurabayashi, Masahiko, E-mail: mkuraba@med.gunma-u.ac.jp [Department of Medicine and Biological Science, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511 (Japan)

    2010-04-02

    Expression of bone-associated proteins and osteoblastic transcription factor Runx2 in arterial cells has been implicated in the development of vascular calcification. However, the signaling upstream of the Runx2-mediated activation of osteoblastic program in vascular smooth muscle cells (VSMC) is poorly understood. We examined the effects of fibroblast growth factor-2 (FGF-2), an important regulator of bone formation, on osteoblastic differentiation of VSMC. Stimulation of cultured rat aortic SMC (RASMC) with FGF-2 induced the expression of the osteoblastic markers osteopontin (OPN) and osteocalcin. Luciferase assays showed that FGF-2 induced osteocyte-specific element (OSE)-dependent transcription. Downregulation of Runx2 by siRNA repressed the basal and FGF-2-stimulated expression of the OPN gene in RASMC. FGF-2 produced hydrogen peroxide in RASMC, as evaluated by fluorescent probe. Induction of OPN expression by FGF-2 was inhibited not only by PD98059 (MEK1 inhibitor) and PP1 (c-Src inhibitor), but also by an antioxidant, N-acetyl cysteine. Nuclear extracts from FGF-2-treated RASMC exhibited increased DNA-binding of Runx2 to its target sequence. Immunohistochemistry of human coronary atherectomy specimens and calcified aortic tissues showed that expression of FGF receptor-1 and Runx2 was colocalized. In conclusion, these results suggest that FGF-2 plays a role in inducing osteoblastic differentiation of VSMC by activating Runx2 through mitogen-activated protein kinase (MAPK)-dependent- and oxidative stress-sensitive-signaling pathways.

  8. Environmental quality of agricultural soils within the Jaguari River Basin - Sao Paulo

    International Nuclear Information System (INIS)

    Ruby, Elaine Cristina

    2009-01-01

    Environmental impacts have occurred in various forms and intensities on soil, water and air media. Consequently, several countries have used legal criteria for soil protection, either by means of generic guiding values or through case-by-case risk assessment. The Sao Paulo Environmental Agency (CETESB) pioneered the publication of guiding values for soils and groundwater in 2001. The aim of this study was to evaluate the environmental quality of agricultural soils in comparison to pristine soils (control areas) within the Jaguari river basin, Sao Paulo. The evaluation was carried out through multielement determination by Neutron Activation Analysis Instrumental (INAA) technique. The analyses were also complemented by Optical Emission Spectrometry Coupled Plasma (ICP OES), Atomic Absorption Spectrometry and Graphite Furnace (GFAAS) techniques. The results obtained in the analyzed soil samples were compared to the guiding values established by the Sao Paulo State environmental legislation and revealed that there were no median concentrations above the prevention values. The median concentrations for the elements Sb, As, Cd, Pb, Co, Cu, Cr, Ni, V and Zn were below the reference values, except for Pb. Taking into account the 34 elements determined, there were statistically significant differences (p <0.05) between agricultural and pristine soils only for the elements Ba, As, U and V. Among these elements, Ba presented the highest concentrations in pristine soils. It was concluded, that the environmental quality of agricultural soils within the Jaguari river basin - SP was slightly changed for the given parameters. The results also pointed out for the utilization of U and As as indicators of potential contamination in soils. (author)

  9. Unified costs for the rural electrification in the state of Sao Paulo, Brazil; Custos unificados para a eletrificacao rural em Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Fernando Selles; Kurahassi, Luiz Fernando; Pazzini, Luiz Henrique Alves; Galvao, Luiz Claudio Ribeiro; Pelegrini, Marcelo Aparecido [Sao Paulo Univ., SP (Brazil). Dept. de Engenharia de Energia e Automacao Eletricas. Grupo de Energia]. E-mail: kurahass@pea.usp.br

    2000-07-01

    The Banco Nacional de Desenvolvimento Economico e Social - BNDES, a Brazilian bank for the economic and social development - worried about the high connections costs practiced by the energy utilities, made some demands to the Sao Paulo State Government, Brazil to become the agent backer of the rural electrification 'Luz da Terra' program. Two of them was the elaboration and adoption of an unified technical norm privileging the adoption of simplified systems of electric energy distribution and an only system of costs establishment compatible with the goals of low medium connection costs for the state. A model of costs establishment was elaborated through the medium prices of market materials and labor activities whose values were more significant in the final composition of the projects. The model was accepted by the utilities participants of the program as a pattern to establish a reference cost for each project to be executed. The advantage of an unified system is that it allows to each project of grid extension to have its costs of materials and labor activities estimated in the same base, independent of the company involved. In spite of not having been adopted by all the utilities ones, the adoption of an unified system and the establishment of reference costs was essential for the control of rural distribution grids prices in the Sao Paulo State. (author)

  10. Upper Devonian (Frasnian) non-calcified, algae, Alberta: Geological relevance to Leduc platforms and petroleum source rocks

    Energy Technology Data Exchange (ETDEWEB)

    Dix, G.R. (Univ. of British Columbia, Vancouver, BC (Canada))

    1990-12-01

    Several types of non-calcified fossil algae comparable to extant brown and green benithic macrophytes occur abundantly on two bedding planes in drill core from argillaceous slope carbonates of the Ireton Formation in northern Alberta. Fossiliferous strata abruptly overlie part of a stepped-back margin of the Sturgeon Lake carbonate platform (Leduc Formation), southeast of the Peace River Arch. Fossils are flattened organic fragments, some representing nearly complete specimens. Tentative comparisons are made with some Paleozoic algae; some of the Sturgeon Lake flora may be new species or genera. Preliminary examination of selected cores from the Ireton Formation and organic-rich Duvernay Formation in central Alberta indicates a widespread distribution of algal-derived organic matter within Upper Devonian basinal strata. The geological relevance of non-calcified algae to Devonian carbonate platforms and basins is postulated in three cases. Their presence in slope sediments may indicate that algal lawns flourished in muddy, upper slope environments. Fossils accumulated either in situ, or were ripped up and quickly buried within downshope resedimented deposits. All or some algal fragments may have been swept from the adjacent carbonate platform during storms. Prolific shallow water algal growth may have occurred simultaneously with oceanic crises when shallow water carbonate production either decreased or was shut down. The present position of fossil algae, therefore, would mark a bedding surface that is stratigraphically equivalent to an intraplatform disconformity. Regardless of the original environment, a sufficient accumulation of non-calcified algae in slope strata represents a viable petroleum source proximal to carbonate platforms. 46 refs., 9 figs.

  11. Dual-energy computed tomography for characterizing urinary calcified calculi and uric acid calculi: A meta-analysis

    International Nuclear Information System (INIS)

    Zheng, Xingju; Liu, Yuanyuan; Li, Mou; Wang, Qiyan; Song, Bin

    2016-01-01

    Objective: A meta-analysis was conducted to determine the accuracy of dual-energy computed tomography (DECT) for differentiating urinary uric acid and calcified calculi. Methods: The databases PubMed, EMBASE, Web of Science, and the Cochrane Library were searched up to May 2016 for relevant original studies. Data were extracted to calculate the pooled sensitivity, specificity, diagnostic odds ratio (OR), positive and negative likelihood ratios (PLR and NLR), and areas under summary receiver operating characteristic (AUROC) curves for analysis. Results: Nine studies (609 stones in 415 patients) were included. For differentiating uric acid (UA) and non-UA calculi with DECT, the analysis indicated: pooled weighted sensitivity, 0.955 (95% CI, 0.888–0.987); specificity, 0.985 (95% CI, 0.970–0.993); PLR, 0.084 (95% CI, 0.041–0.170); NLR 33.327 (95% CI, 18.516–59.985); and diagnostic OR 538.18 (95% CI, 195.50–1478.5). The AUROC value was 0.9901. For calcified stones, the analysis indicated: pooled weighted sensitivity, 0.994 (95% CI, 0.969–1); specificity, 0.973 (95% CI, 0.906–0.997); PLR, 11.200 (95% CI, 4.922–25.486); NLR 0.027 (95% CI, 0.010–0.072); and diagnostic OR 654.89 (95% CI, 151.31–2834.4). The AUROC value was 0.9915. Conclusion: This meta-analysis found that DECT is a highly accurate noninvasive method for characterizing urinary uric acid and calcified calculi.

  12. Dual-energy computed tomography for characterizing urinary calcified calculi and uric acid calculi: A meta-analysis

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Xingju; Liu, Yuanyuan; Li, Mou; Wang, Qiyan; Song, Bin, E-mail: binsong65@yahoo.com

    2016-10-15

    Objective: A meta-analysis was conducted to determine the accuracy of dual-energy computed tomography (DECT) for differentiating urinary uric acid and calcified calculi. Methods: The databases PubMed, EMBASE, Web of Science, and the Cochrane Library were searched up to May 2016 for relevant original studies. Data were extracted to calculate the pooled sensitivity, specificity, diagnostic odds ratio (OR), positive and negative likelihood ratios (PLR and NLR), and areas under summary receiver operating characteristic (AUROC) curves for analysis. Results: Nine studies (609 stones in 415 patients) were included. For differentiating uric acid (UA) and non-UA calculi with DECT, the analysis indicated: pooled weighted sensitivity, 0.955 (95% CI, 0.888–0.987); specificity, 0.985 (95% CI, 0.970–0.993); PLR, 0.084 (95% CI, 0.041–0.170); NLR 33.327 (95% CI, 18.516–59.985); and diagnostic OR 538.18 (95% CI, 195.50–1478.5). The AUROC value was 0.9901. For calcified stones, the analysis indicated: pooled weighted sensitivity, 0.994 (95% CI, 0.969–1); specificity, 0.973 (95% CI, 0.906–0.997); PLR, 11.200 (95% CI, 4.922–25.486); NLR 0.027 (95% CI, 0.010–0.072); and diagnostic OR 654.89 (95% CI, 151.31–2834.4). The AUROC value was 0.9915. Conclusion: This meta-analysis found that DECT is a highly accurate noninvasive method for characterizing urinary uric acid and calcified calculi.

  13. Anthropic contribution on pollution in hydroelectric reservoirs: the case of Sao Simao's hydroelectric power plant - GO/MG, Brazil; Contribuicao antropica na poluicao de reservatorios hidreletricos: o caso da Usina Hidreletrica de Sao Simao - GO/MG

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Gizele Araujo Borba da

    2010-02-15

    This study aimed to evaluate the contribution of anthropogenic pollution in hydroelectric reservoirs through a case study of the Sao Simao Hydropower. The literature relates the problem of eutrophication with the addition of nutrients (nitrogen and phosphorus) in excess, resulting in water pollution. Through data for both tributary rivers and the reservoir, it was found that the Sao Simao dam presents a framework for eutrophication. We made an analysis to see which tributary rivers were the main sources of nutrients released into the waters of the reservoir and a survey of possible human activities that are contributing to the intake of nutrients in these tributaries. (author)

  14. The strength of a calcified tissue depends in part on the molecular structure and organization of its constituent mineral crystals in their organic matrix

    Science.gov (United States)

    Landis, W. J.

    1995-01-01

    High-voltage electron-microscopic tomographic (3D) studies of the ultrastructural interaction between mineral and organic matrix in a variety of calcified tissues reveal different crystal structural and organizational features in association with their respective organic matrices. In brittle or weak pathologic or ectopic calcifications, including examples of osteogenesis imperfecta, calciphylaxis, calcergy, and dermatomyositis, hydroxyapatite crystals occur in various sizes and shapes and are oriented and aligned with respect to collagen in a manner which is distinct from that found in normal calcified tissues. A model of collagen-mineral interaction is proposed which may account for the observed crystal structures and organization. The results indicate that the ultimate strength, support, and other mechanical properties provided by a calcified tissue are dependent in part upon the molecular structure and arrangement of its constituent mineral crystals within their organic matrix.

  15. Cardiac Calcified Amorphous Tumor of the Mitral Valve Presenting as Transient Ischemic Attack.

    Science.gov (United States)

    Abbasi Teshnizi, Mohammad; Ghorbanzadeh, Atefeh; Zirak, Nahid; Manafi, Babak; Moeinipour, Aliasghar

    2017-01-01

    Cardiac calcified amorphous tumors (CATs) are an extremely rare nonneoplastic intracardiac masses. They have been reported in the literature in only a few cases. Thus, the incidence, pathogenesis, and best approach to the treatment are not certain. We report a case of CATs on the atrial surface of the anterior mitral valve leaflet in a 37-year-old female who was diagnosed by histopathological examination after surgical removal.

  16. Cardiac Calcified Amorphous Tumor of the Mitral Valve Presenting as Transient Ischemic Attack

    Directory of Open Access Journals (Sweden)

    Mohammad Abbasi Teshnizi

    2017-01-01

    Full Text Available Cardiac calcified amorphous tumors (CATs are an extremely rare nonneoplastic intracardiac masses. They have been reported in the literature in only a few cases. Thus, the incidence, pathogenesis, and best approach to the treatment are not certain. We report a case of CATs on the atrial surface of the anterior mitral valve leaflet in a 37-year-old female who was diagnosed by histopathological examination after surgical removal.

  17. A study on elemental composition in epiphytic lichen samples used as bioindicator of air pollution in Sao Paulo city

    International Nuclear Information System (INIS)

    Montezani, Edmila

    2010-01-01

    Studies on air pollution have intensified in recent years, due to the diversity of emissions and the effect caused to the health of populations. Consequently, several techniques have been investigated for air pollution evaluation and among them one that has gained considerable attention is that of biomonitoring. In this study chemical elemental levels in the atmosphere of Sao Paulo city were evaluated, by means of passive biomonitoring, using epiphytic Canoparmelia texana species, in order to compare between the results obtained in samples from different sites of Sao Paulo city and in a reference site of Ubatuba city, SP. Neutron activation analysis (NAA) procedure applied in the analyses consisted of irradiating aliquots of samples along with synthetic standards of elements in the nuclear reactor IEA-R1 for 16 hours under a thermal neutron flux of about 5.0 x 10 12 n cm -2 s -1 , followed by gamma ray spectrometry for the determination of As, Br, Ca, Co, Cr, Cs, Fe, K, La, Rb, Sc, Se and Zn. Results obtained in the analyses of lichens samples in replicates presented good reproducibility indicating homogeneity of the prepared samples. The precision and accuracy of the results were evaluated by the analyses of certified reference materials IAEA-336 Lichen and INCT-TL-1 Tea Leaves. Results obtained in the reference materials presented, in general, good precision, with relative standard deviations between 0.4 and 14.8% and good accuracy with relative errors between 0.2 and 8.7%. In Sao Paulo city, the lichens were collected in the following sites: Parque Dom Pedro II, Congonhas, Cidade Universitaria, Lapa, Mooca, Morumbi, Nossa Senhora do O, Parque Ibirapuera, Pinheiros, Santana, Santo Amaro and Taboao da Serra. Element concentrations found in lichens indicated a great variability depending on the sites where the samples were collected. The results obtained in the lichens submitted to the cluster analysis indicated three groups of sampling sites according to the

  18. A study on elemental composition in epiphytic lichen samples used as bioindicator of air pollution in Sao Paulo city; Estudo sobre a composicao elementar nas amostras de liquen epifitico utilizado como bioindicador da poluicao aerea na cidade de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Montezani, Edmila

    2010-07-01

    Studies on air pollution have intensified in recent years, due to the diversity of emissions and the effect caused to the health of populations. Consequently, several techniques have been investigated for air pollution evaluation and among them one that has gained considerable attention is that of biomonitoring. In this study chemical elemental levels in the atmosphere of Sao Paulo city were evaluated, by means of passive biomonitoring, using epiphytic Canoparmelia texana species, in order to compare between the results obtained in samples from different sites of Sao Paulo city and in a reference site of Ubatuba city, SP. Neutron activation analysis (NAA) procedure applied in the analyses consisted of irradiating aliquots of samples along with synthetic standards of elements in the nuclear reactor IEA-R1 for 16 hours under a thermal neutron flux of about 5.0 x 10{sup 12} n cm{sup -2} s{sup -1}, followed by gamma ray spectrometry for the determination of As, Br, Ca, Co, Cr, Cs, Fe, K, La, Rb, Sc, Se and Zn. Results obtained in the analyses of lichens samples in replicates presented good reproducibility indicating homogeneity of the prepared samples. The precision and accuracy of the results were evaluated by the analyses of certified reference materials IAEA-336 Lichen and INCT-TL-1 Tea Leaves. Results obtained in the reference materials presented, in general, good precision, with relative standard deviations between 0.4 and 14.8% and good accuracy with relative errors between 0.2 and 8.7%. In Sao Paulo city, the lichens were collected in the following sites: Parque Dom Pedro II, Congonhas, Cidade Universitaria, Lapa, Mooca, Morumbi, Nossa Senhora do O, Parque Ibirapuera, Pinheiros, Santana, Santo Amaro and Taboao da Serra. Element concentrations found in lichens indicated a great variability depending on the sites where the samples were collected. The results obtained in the lichens submitted to the cluster analysis indicated three groups of sampling sites

  19. Quality standards proposal for the pipeline gas distribution service in the state of Sao Paulo - Brazil; Proposta de padroes de qualidade para o servico de distribuicao de gas canalizado no Estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Galvao, Clovis Badaro; Assad, Luiz Sergio [Secretaria de Estado de Energia, Sao Paulo, SP (Brazil); Bajay, Sergio Valdir [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Mecanica

    1998-12-31

    The Energy Supply Public Utilities Commission of the State of Sao Paulo - CSPE created in April 1998 to regulate the public services of electricity and gas distribution through networks in the State. Within its legal attributions, the future staff of CSPE, still in the Sao Paulo Secretary of State of Energy, put forward standards to regulate the quality of service in the distribution of electricity and pipeline gas in the State. This paper summarises the current, still preliminary, version of these gas standards, that take into account the current practice of COMGAS, international, well proven, standards as well as COMGAS capacity to achieve quality of service gains favoring the consumers. (author) 8 refs., 3 tabs.

  20. Crescimento de pitaia vermelha com adubação orgânica e granulado bioclástico Growth of red pitaya with organic fertilizer and calcified seaweed

    Directory of Open Access Journals (Sweden)

    Rodrigo Amato Moreira

    2011-05-01

    Full Text Available Objetivou-se com o trabalho avaliar o crescimento de pitaia vermelha (Hylocereus undatus submetida à adubação orgânica e à aplicação de granulado bioclástico. O delineamento experimental foi em blocos casualizados com oito adubações: testemunha, esterco de curral, cama de frango, granulado bioclástico, esterco de curral + cama de frango, esterco de curral + granulado bioclástico, cama de frango + granulado bioclástico e esterco de curral + cama de frango + granulado bioclástico, aplicados a cada três meses, com três blocos, e a parcela experimental composta por quatro plantas. Não foram observadas diferenças significativas no comprimento e no número de cladódios principais. Houve diferença significativa no número de cladódios laterais retirados e de cladódios emitidos acima do suporte. A adubação com esterco de curral + cama de frango + granulado bioclástico favoreceu o crescimento de plantas de pitaia vermelha.The objective of the study was to evaluate the growth of red pitaya (Hylocereus undatus subjected to organic fertilization and calcified seaweed application. The experimental design was in randomized blocks with eight fertilization: control, cattle manure, chicken manure, calcified seaweed, cattle manure + chicken manure, cattle manure + calcified seaweed, chicken manure + calcified seaweed and cattle manure + chicken manure + calcified seaweed, applied every three months, with three replications and plot consisted of four plants. There were no significant differences in the length and in the number of central cladodes. There were significant differences in the number of side cladodes removed and in cladodes above the support. Fertilization with cattle manure + chicken manure + calcified seaweed favored the growth of red pitaya.

  1. Arthroscopic Removal and Rotator Cuff Repair Without Acromioplasty for the Treatment of Symptomatic Calcifying Tendinitis of the Supraspinatus Tendon.

    Science.gov (United States)

    Ranalletta, Maximiliano; Rossi, Luciano Andrés; Bongiovanni, Santiago Luis; Tanoira, Ignacio; Piuzzi, Nicolas; Maignon, Gastón

    2015-04-01

    Calcified rotator cuff tendinitis is a common cause of chronic shoulder pain that leads to significant pain and functional limitations. Although most patients respond well to conservative treatment, some eventually require surgical treatment. To evaluate the clinical outcome with arthroscopic removal of calcific deposit and rotator cuff repair without acromioplasty for the treatment of calcific tendinitis of the supraspinatus tendon. Case series; Level of evidence, 4. This study retrospectively evaluated 30 consecutive patients with a mean age of 49.2 years. The mean follow-up was 35 months (range, 24-88 months). Pre- and postoperative functional assessment was performed using the Constant score, University of California Los Angeles (UCLA) score, and Quick Disabilities of the Arm, Shoulder, and Hand (DASH). Pain was assessed by visual analog scale (VAS). Radiographs and magnetic resonance imaging (MRI) were performed to evaluate the recurrence of calcifications and the indemnity of the supraspinatus tendon repair. Significant improvement was obtained for pain (mean VAS, 8.7 before surgery to 0.8 after; P rotator cuff repair without acromioplasty can lead to good results in patients with symptomatic calcifying tendonitis of the supraspinatus tendon.

  2. Calcifying epithelial odontogenic tumor of the posterior maxilla

    Directory of Open Access Journals (Sweden)

    Vidya Ajila

    2016-01-01

    Full Text Available Calcifying epithelial odontogenic tumor (CEOT is a rare odontogenic neoplasm comprising <1% of all odontogenic tumors. It is commonly seen in the third to fifth decades of life without any gender predilection. It usually occurs in the mandibular posterior region. A painless, slow growing swelling with bone expansion is the most common clinical feature of CEOT. Radiographically, it presents as a mixed lesion with or without an associated impacted tooth. Confirmation of the diagnosis is by histopathological examination. We describe an unusual case of CEOT occurring in the maxillary posterior region and involving the maxillary sinus. The associated impacted third molar was displaced to the lateral wall of the nose and root resorption was seen in all the teeth associated with the lesion. There was no evidence of calcification in conventional as well as computed tomography images.

  3. In vitro cytotoxicity of galvanically coupled magnesium-titanium particles on human osteosarcoma SAOS2 cells: A potential cancer therapy.

    Science.gov (United States)

    Kim, Jua; Gilbert, Jeremy L

    2018-04-10

    Osteosarcoma is a malignant bone cancer that occurs mostly in children and young adults. This study investigated the cytotoxicity of Mg and Mg-Ti microparticles to human osteosarcoma cells. Osteosarcoma cells were killed in a dosage-dependent manner when cells, with a cell seeding density of 30,000 cells/cm 2 , were cultured with 0 to 2500 µg/mL of Mg or Mg-Ti in cell culture media for 24-72 h. Mg-Ti killed cells more effectively, where 1250 µg/mL of Mg-Ti killed cells completely by 24 h, while 2500 µg/mL of Mg killed nearly all cells, but not all. Killing due to particle corrosion occurred mostly during the first 24 h, and so the percent cell viability between 24 and 72 h showed not much variability. However, the measurement of live and dead cell numbers, over the timeframe of 24-72 h, showed more insight, such as cell recovery. If particle concentrations were low, the number of live cells increased after 24 h, indicating cell proliferation. If particle concentrations were high, the number of live cells either remained steady or decreased, indicating cell quiescence or continued killing, respectively. Increase in the number of dead cells also indicated killing, while plateau meant discontinued killing. In addition, repeated killing of recovered cells exhibited the same dose-dependent killing profile as the initial experiment, implying little development of cell resistance to treatment. These results, together, show that osteosarcoma cells are susceptible to killing by way of exposure to corroding particles, showing highly effective killing using the galvanic couple of Mg-Ti. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018. © 2018 Wiley Periodicals, Inc.

  4. Osteoclasts but not osteoblasts are affected by a calcified surface treated with zoledronic acid in vitro

    International Nuclear Information System (INIS)

    Schindeler, Aaron; Little, David G.

    2005-01-01

    Bisphosphonates are potent inhibitors of osteoclast-mediated bone resorption. Recent interest has centered on the effects of bisphosphonates on osteoblasts. Chronic dosing of osteoblasts with solubilized bisphosphonates has been reported to enhance osteogenesis and mineralization in vitro. However, this methodology poorly reflects the in vivo situation, where free bisphosphonate becomes rapidly bound to mineralized bone surfaces. To establish a more clinically relevant cell culture model, we cultured bone cells on calcium phosphate coated quartz discs pre-treated with the potent nitrogen-containing bisphosphonate, zoledronic acid (ZA). Binding studies utilizing [ 14 C]-labeled ZA confirmed that the bisphosphonate bound in a concentration-dependent manner over the 1-50 μM dose range. When grown on ZA-treated discs, the viability of bone-marrow derived osteoclasts was greatly reduced, while the viability and mineralization of the osteoblastic MC3T3-E1 cell line were largely unaffected. This suggests that only bone resorbing cells are affected by bound bisphosphonate. However, this system does not account for transient exposure to unbound bisphosphonate in the hours following a clinical dosing. To model this event, we transiently treated osteoblasts with ZA in the absence of a calcified surface. Osteoblasts proved highly resistant to all transitory treatment regimes, even when utilizing ZA concentrations that prevented mineralization and/or induced cell death when dosed chronically. This study represents a pharmacologically more relevant approach to modeling bisphosphonate treatment on cultured bone cells and implies that bisphosphonate therapies may not directly affect osteoblasts at bone surfaces

  5. The geotectonic evolution of southern part of Sao Francisco Craton, based in geochronologic interpretation

    International Nuclear Information System (INIS)

    Teixeira, W.

    1985-01-01

    Interpretation of available radiometric data from poly metamorphic terranes of southern part of the Sao Francisco Craton demonstrates the importance of geochronology as a tool in the study of ancient crustal evolution. In addition, radiometric study of basic intrusive magmatism helps define the most important epochs of crustal rifting during the Proterozoic. The definition of the southern border of the cratonic area based on distinctive age patterns of the geochronological provinces is also discussed. Finally, the geochronologic evolution of the Bambui platform cover is presented. Approximately 250 radiometric age determinations (Rb-Sr, K-Ar and Pb-Pb methods) were interpreted principally through the use of iso chronic diagrams. The geologic history tectonomagnetic events identified in this study is compared to the crustal evolution of similar segments of the Sao Francisco Craton and elsewhere. (author)

  6. Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings

    Czech Academy of Sciences Publication Activity Database

    Filová, Elena; Vandrovcová, Marta; Jelínek, Miroslav; Zemek, Josef; Houdková, Jana; Remsa, Jan; Kocourek, Tomáš; Staňková, Ľubica; Bačáková, Lucie

    2017-01-01

    Roč. 28, č. 1 (2017), č. článku 17. ISSN 0957-4530 R&D Projects: GA ČR(CZ) GA15-05864S; GA ČR(CZ) GA14-04790S; GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:67985823 ; RVO:68378271 Keywords : osteocalcin * osteogenic differentiation * hexavalent chromium * focal adhesion contact * cell spreading area Subject RIV: EI - Biotechnology ; Bionics OBOR OECD: Biomaterials (as related to medical implants, devices, sensors) Impact factor: 2.325, year: 2016

  7. Effective Endovascular Treatment of Calcified Femoropopliteal Disease With Directional Atherectomy and Distal Embolic Protection: Final Results of the DEFINITIVE Ca++ Trial

    Science.gov (United States)

    Roberts, David; Niazi, Khusrow; Miller, William; Krishnan, Prakash; Gammon, Roger; Schreiber, Theodore; Shammas, Nicolas W; Clair, Daniel

    2014-01-01

    Objectives The purpose of the DEFINITIVE Ca++ study was to evaluate the safety and effectiveness of directional atherectomy and distal embolic protection, used together to treat moderate to severely calcified femoropopliteal lesions. Background Despite advances in endovascular treatment modalities, treatment of calcified lesions remains a challenge. Methods A total of 133 subjects with 168 moderate to severely calcified lesions were enrolled. Lesions were treated with directional atherectomy devices, coupled with distal embolic protection. Results The 30-day freedom from MAE rate was 93.1%. Per angiographic core laboratory assessment, the primary effectiveness endpoint (≤50% residual diameter stenosis) was achieved in 92.0% (lower confidence bound of 87.6%) of lesions. By core lab analysis, these results did not achieve the success criteria (90%) for the primary effectiveness objective. Per site assessment, the objective was met with the endpoint being achieved in 97.0% (lower confidence bound 93.8%). A mean residual diameter stenosis of 33.3% was achieved with the directional atherectomy device. This was further decreased to 24.1% with the use of adjunctive therapy. The proportion of asymptomatic subjects [Rutherford Clinical Category (RCC) = 0] increased from 0% at baseline to 52.3% at the 30-day follow-up visit. In total, 88.5% of subjects experienced an improvement of one or more Rutherford categories. Conclusions The results of the DEFINITIVE Ca++ study demonstrate that the SilverHawk™ and TurboHawk™ atherectomy devices are safe and effective in the endovascular treatment of moderate to severely calcified lesions in the superficial femoral and/or popliteal arteries when used with the SpiderFX™ distal embolic protection device. © 2014 Wiley Periodicals, Inc. PMID:24402764

  8. The tectonic evolution of Southern part of the Sao Francisco Craton

    International Nuclear Information System (INIS)

    Teixeira, W.

    1986-01-01

    The potentiality of geochronology when it is applied to the geologic context of craton basement of archean areas is shown. Samples from southern part of the Sao Francisco Craton, in Brazil, were collected for petrographic analysis and geochronological data interpretation. The set of radiometric determinations was obtained by K-Ar, Rb-Sr, Pb-Pb and U-Pb methods. (M.C.K.) [pt

  9. Physicochemical characterization of ceramics from Sao Paulo II archaeological site; Caracterizacao fisico-quimica da ceramica do sitio arqueologico Sao Paulo II

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Rogerio Baria

    2013-08-01

    Archaeometry is a consolidated field with a wide application of nuclear analytical techniques for the characterization, protection, and restoration of archaeological pieces. This project aimed at studying the elementary chemical composition of 70 ceramic fragments samples from Sao Paulo II archaeological site, located along the Solimoes River channel, next to Coari city, in Brazilian Amazon. The characterization of samples was performed by neutron activation analysis (NAA). By the determination of 24 elements in the ceramic fragments ( Ce, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Na, Nd, Sb, Sm. Rb, Se, Ta, Tb, Th, U, Yb and Zn), it was possible to define groups of samples regarding the similarity/dissimilarity in elementary chemical composition. For such a task, the multivariate statistical methods employed were cluster analysis (C A), principal component analysis (PCA) and discriminant analysis (DA). Afterwards, seven ceramic fragments were selected based on the groups previously established, for the characterization of the site temporal horizon. Those ceramic fragments were analyzed by thermoluminescence (TL) and EPR for dating purposes. The firing temperatures were determined by electron paramagnetic resonance (EPR) technique, in order to infer about some aspects of the ceramic manufacture employed by the ancient peoples that lived in Sao Paulo 11. By the results obtained in this study, it was possible to identify the quantity of clay sources employed by the ceramists and the age of the ceramic pieces. Therefore, the results of this research may contribute to the study on the occupation dynamics in the pre-colonial Brazilian Amazon. (author)

  10. Anthropic contribution on pollution in hydroelectric reservoirs: the case of Sao Simao's hydroelectric power plant - GO/MG, Brazil; Contribuicao antropica na poluicao de reservatorios hidreletricos: o caso da Usina Hidreletrica de Sao Simao - GO/MG

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Gizele Araujo Borba da

    2010-02-15

    This study aimed to evaluate the contribution of anthropogenic pollution in hydroelectric reservoirs through a case study of the Sao Simao Hydropower. The literature relates the problem of eutrophication with the addition of nutrients (nitrogen and phosphorus) in excess, resulting in water pollution. Through data for both tributary rivers and the reservoir, it was found that the Sao Simao dam presents a framework for eutrophication. We made an analysis to see which tributary rivers were the main sources of nutrients released into the waters of the reservoir and a survey of possible human activities that are contributing to the intake of nutrients in these tributaries. (author)

  11. Modulation of intracellular calcium and proliferative activity of invertebrate and vertebrate cells by ethylene

    Directory of Open Access Journals (Sweden)

    Müller Werner EG

    2001-05-01

    Full Text Available Abstract Background Ethylene is a widely distributed alkene product which is formed enzymatically (e.g., in plants or by photochemical reactions (e.g., in the upper oceanic layers from dissolved organic carbon. This gaseous compound was recently found to induce in cells from the marine sponge Suberites domuncula, an increase in intracellular Ca2+ level ([Ca2+]i and an upregulation of the expression of two genes, the potential ethylene-responsive gene, SDERR, and a Ca2+/calmodulin-dependent protein kinase. Results Here we describe for the first time, that besides sponge cells, mammalian cell lines (mouse NIH-3T3 and human HeLa and SaOS-2 cells respond to ethylene, generated by ethephon, with an immediate and strong, transient increase in [Ca2+]i level, as demonstrated using Fura-2 imaging method. A rise of [Ca2+]i level was also found following exposure to ethylene gas of cells kept under pressure (SaOS-2 cells. The upregulation of [Ca2+]i was associated with an increase in the level of the cell cycle-associated Ki-67 antigen. In addition, we show that the effect of ethephon addition to S. domuncula cells depends on the presence of calcium in the extracellular milieu. Conclusion The results presented in this paper indicate that ethylene, previously known to act as a mediator (hormone in plants only, deserves also attention as a potential signaling molecule in higher vertebrates. Further studies are necessary to clarify the specificity and physiological significance of the effects induced by ethylene in mammalian cells.

  12. Effects of vinasse accumulation ponds and decantation reservoirs of water used for washing cane sugar in water resources in Dobrada municipality, Sao Paulo State, Brazil; Efeitos da estocagem de vinhaca e das aguas de lavagem da cana-de-acucar na qualidade dos recursos hidricos da regiao de Dobrada, Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Sabadia, Jose Antonio Beltrao; Reboucas, Aldo da Cunha [Sao Paulo Univ., SP (Brazil). Inst. de Geociencias

    1996-12-31

    This work shows the results of a monitoring carried out in an area of about 21 km{sup 2}, Dobrada Municipality, central west region of the state of Sao Paulo (Brazil), where the impacts in ground water and surface water were evaluated by the infiltration and percolation of vinasse and water used for washing cane sugar, through accumulation ponds and decantation reservoirs, respectively, in areas of Bauru Aquifer (Cretaceous). The Bauru Aquifer, free and porous, occurs in a extended area of 104.000 km{sup 2} in Sao Paulo State, as a major exploitable aquifer (more than 15.000 wells), used for domestic and industrial water supplies. For the groundwater, the following measures were found in analyses exceeding drinking water standards (WHO; CONAMA/Brazil and Sao Paulo State): manganese (0,03 to 3,5 mg/l), iron (0.45 to 34 mg/l), aluminum (1 to 52 mg/l) and phosphate (0,03 to 0.38 mg/l). For superficial water, the following measures exceeded drinking water standards: total iron (3 mg/l) and phosphate (0,035 mg/l). The main purpose of this research is to improve the field methodology to characterize the impacts of vinasse and washing water of cane sugar infiltration/percolation., detaching the relationship between ground and surface water, with the major aim to supply the legislation to protect drinking water resources. (author) 11 refs.

  13. Geology and geochronology of Cardoso Island, in the southeastern coast of Sao Paulo State; Geologia e geocronologia da Ilha de Cardoso, sudeste do Estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Weber, Werner

    1998-07-01

    This aim of work is the geological and geochronological study of rocks cropping out on Cardoso Island, on the southeastern coast of Sao Paulo States, close to be boundary with Parana State. The Island with an area of about 151 km{sup 2} is a protected area administered by the Forest Institute of Environment Secretariat of the State of Sao Paulo. It is mountanious, with a peak at 814 m, and is covered by dense Mata Atlantica vegetation. The terrains which compose the island are mainly an igneous complex with light grey leucocratic, inequigranular, medium - to coarse-grained syenites. The predominant Tres Irmaos Syenite (STI), composed of pyroxene, hornblende, and perthitic to mesoperthitic microcline, has a magmatic flow structures, and is cut by the Cambriu alkali-feldspar Granites (GC), which is pinkish grey, leucocratic and medium-grained. Geochemical analysis of STI and GC demonstrate their meta luminous alkaline nature and late-orogenic to an orogenic character. The geochronological results suggest that the bodies were formed between 620 and 570 My according to the U-Pb method in zircons, with cooling between 597 and 531 My (K-Ar in amphiboles). Whole rock Sm-Nd analysis yield T{sub DM} ages in the Meso and Paleoproterozoic (1.200 - 2.200 My). belt of low grade meta sedimentary rocks occurs in the northern part of the island. Quartz schist, quartz-mica schist and mica-quartz schist, often containing andaluzite and cordierite, predominate. The geochemical and geochronological data suggest that the sources of the metasediments were andesites of continental arc whose protolities separated from the mantle during the Paleoproterozoic, between 1.800 and 2.200 My. These metasediments probably continue on the continent in the Taquari region and extend southwards in narrow strips between the granitoids of the Paranagua Domain. Although quaternary deposits are expressive, they were not studied in details since they were not the objectives of this study. (author)

  14. Combination of etoposide and fisetin results in anti-cancer efficiency against osteosarcoma cell models.

    Science.gov (United States)

    Ferreira de Oliveira, José Miguel P; Pacheco, Ana Rita; Coutinho, Laura; Oliveira, Helena; Pinho, Sónia; Almeida, Luis; Fernandes, Eduarda; Santos, Conceição

    2018-03-01

    Osteosarcoma chemotherapy is often limited by chemoresistance, resulting in poor prognosis. Combined chemotherapy could, therefore, be used to prevent resistance to chemotherapeutics. Here, the effects of fisetin on osteosarcoma cells were investigated, as well as cytostatic potential in combination with the anti-cancer drug etoposide. For this, different osteosarcoma cell lines were treated with fisetin, with etoposide and with respective combinations. Fisetin was associated with decrease in colony formation in Saos-2 and in U2OS cells but not in MG-63 cells. Notwithstanding, upon evaluation of cellular growth by crystal violet assay, MG-63 and Saos-2 cells showed decreased cell proliferation at 40 and 20 µM fisetin, respectively. Depending on the relative concentrations, fisetin:etoposide combinations showed negative-to-positive interactions on the inhibition of cell proliferation. In addition, fisetin treatment up to 50 µM for 48 h resulted in G2-phase cell cycle arrest. Regardless of the combination, fisetin:etoposide increased % cells in G2-phase and decreased % cells in G1-phase. In addition, mixtures with more positive combined effects induced increased % cells in S-phase. Compared to etoposide treatment, these combinations resulted in decreased levels of cyclins B1 and E1, pointing to the role of these regulators in fisetin-induced cell cycle arrest. In conclusion, these results show that the combination of fisetin with etoposide has higher anti-proliferative effects in osteosarcoma associated with cell cycle arrest, allowing the use of lower doses of the chemotherapeutic agent, which has important implications for osteosarcoma treatment.

  15. Calcified pleural scars and pleural empyema with mural calcification

    Energy Technology Data Exchange (ETDEWEB)

    Schmitt, W.G.H.; Huebener, K.H.

    1981-06-01

    The differential diagnosis between calcified pleural scars and pleural empyemas with mural calcification was studied by computer tomography, bearing in mind the patient's history. In view of the high complication rate of pleural empyemas, such as internal or external fistulae, it is desirable to elucidate every form of pleural shadowing which is more than 20 mm thick. Criteria are offered, which permit the differentiation of the pleural changes by means of conventional radiological examinations. Valuable additional information can be obtained by computer tomography. Forty-nine patients with calcification in the pleura were found among 1.900 chest x-rays which had been examined. Out of these, seven had a pleural empyema. In one case an echinococcus cyst with mural calcification was punctured under X-ray control.

  16. Calcified pleural scars and pleural empyema with mural calcification

    International Nuclear Information System (INIS)

    Schmitt, W.G.H.; Huebener, K.H.

    1981-01-01

    The differential diagnosis between calcified pleural scars and pleural empyemas with mural calcification was studied by computer tomography, bearing in mind the patient's history. In view of the high complication rate of pleural empyemas, such as internal or external fistulae, it is desirable to elucidate every form of pleural shadowing which is more than 20 mm thick. Criteria are offered, which permit the differentiation of the pleural changes by means of conventional radiological examinations. Valuable additional information can be obtained by computer tomography. Forty-nine patients with calcification in the pleura were found among 1.900 chest x-rays which had been examined. Out of these, seven had a pleural empyema. In one case an echinococcus cyst with mural calcification was punctured under X-ray control. (orig.) [de

  17. Diagnostic Performance of Breast Magnetic Resonance Imaging in Non-Calcified Equivocal Breast Findings: Results from a Systematic Review and Meta-Analysis.

    Directory of Open Access Journals (Sweden)

    Barbara Bennani-Baiti

    Full Text Available To evaluate the performance of MRI for diagnosis of breast cancer in non-calcified equivocal breast findings.We performed a systematic review and meta-analysis of peer-reviewed studies in PubMed from 01/01/1986 until 06/15/2015. Eligible were studies applying dynamic contrast-enhanced breast MRI as an adjunct to conventional imaging (mammography, ultrasound to clarify equivocal findings without microcalcifications. Reference standard for MRI findings had to be established by histopathological sampling or imaging follow-up of at least 12 months. Number of true or false positives and negatives and other characteristics were extracted, and possible bias was determined using the QUADAS-2 applet. Statistical analyses included data pooling and heterogeneity testing.Fourteen out of 514 studies comprising 2,316 lesions met our inclusion criteria. Pooled diagnostic parameters were: sensitivity (99%, 95%-CI: 93-100%, specificity (89%, 95%-CI: 85-92%, PPV (56%, 95%-CI: 42-70% and NPV (100%, 95%-CI: 99-100%. These estimates displayed significant heterogeneity (P<0.001.Breast MRI demonstrates an excellent diagnostic performance in case of non-calcified equivocal breast findings detected in conventional imaging. However, considering the substantial heterogeneity with regard to prevalence of malignancy, problem solving criteria need to be better defined.

  18. Guidelines for the development and environmental protection of the outcrop area in the Guarani aquifer system in Sao Paulo State, Brazil; Diretrizes para o desenvolvimento e protecao ambiental da area de afloramento do Sistema Aquifero Guarani no estado de Sao Paulo, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Alburquerque Filho, J. L.; Carvalho, A. M. de; Ikematsu, P.; Costa Barbosa, M.; Iritani, M.; Nogueira Pressinoti, M. M.; Rocha, G.; Pereira Militao da Silva, M.; Theodorovicz, A.

    2012-11-01

    The Guarani aquifer system (GAS) is an important public water source. The unconfined areas are those where the aquifer is more vulnerable to contamination, justifying the creation of a Protection and Recovery Source Area (Area de Protecao e Recuperacao de Manancial - APRM), as established in Act 9.866/97 of Sao Paulo State. We present here the results of the project Environmental Diagnosis in Support of the Plan for the Development and Environmental Protection Plan of the Outcrop Area of the Guarani Aquifer System in Sao Paulo State (Plano de Desenvolvimento e Protecao Ambiental da Area de Afloramento do Sistema Aquifero Guarani no Estado de Sao Paulo PDPA-SAG) and describe the areas of intervention defined by this law. The characterization of the area studied focuses on the physical and socio-economic aspects of the region, in addition to the pertinent legislation. Partial results indicate that the condition of the aquifer is satisfactory, but territorial discipline continues to be of prime importance. (Author)

  19. A calcified cervical intervertebral disc in a child and a thoracic disc calcification in an adult with posterior herniation-radiographic, computed tomography and magnetic resonance imaging findings

    International Nuclear Information System (INIS)

    Jevtic, V.

    2004-01-01

    Background. Nucleus pulposus calcification in children is a relatively rare but well known clinical syndrome, usually localized at the level of the cervical spine. The exact aetiology still remains uncertain. Calcifications of the intervertebral discs in adults differ from the childhood variety. They are mainly degenerative in nature and occur at the level of midthoracic and upper lumbar spine. Potentially serious complications, posterior herniation of calcified disc may occur in both entities. Case reports. We report two cases of the calcification of the nucleus pulposus in a seven-year-old boy at the level of C7-T1 and a case of calcified intervertebral disc T11-T12 in a forty-five-year-old woman, with massive posterior herniation. Remission of symptoms was achieved with a conservative therapy alone. Clinical, radiographic, computed tomographic and magnetic resonance imaging (MRI) findings were analyzed in an attempt to investigate similarities and differences between both disease entities. Conclusion. Massive posterior herniation of calcified nucleus pulposus in a child was treated conservatively with a favourable outcome. A disappearance of symptoms followed quick resolution of herniated calcified masses. In adult variety extruded thoracic disc calcification was of a permanent type with no tendency towards spontaneous resolution and remission of symptoms after the conservative therapy. MRI seems to be able to depict disc calcification before a conventional radiography. The widening of affected discs in a paediatric patient was also better demonstrated by MRI. It would seem to support the theory of an increased intradiscal pressure as the precursor of annulus fibrosus ruptures and consecutive calcified disc herniations. (author)

  20. Cell adhesion on Ti surface with controlled roughness

    Directory of Open Access Journals (Sweden)

    Burgos-Asperilla, Laura

    2015-06-01

    Full Text Available In this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM and electrochemical impedance spectroscopy (EIS. The rate of Saos-2 attachment on Ti surfaces, obtained from the measurements performed with the QCM, is a first-order reaction, with k=2.10−3 min−1. The impedance measurements indicate that in the absence of cells, the Ti resistance diminishes over time (7 days, due to the presence of amino acids and proteins from the culture medium that have been a dsorbed, while in the presence of osteoblasts, this decrease is much greater because of the compounds generated by the cells that accelerate the dissolution of Ti.En este trabajo, se ha estudiado la interacción in situ entre células osteoblásticas Saos-2 y una superficie de Ti de rugosidad controlada a lo largo del tiempo. El estudio de la cinética y los mecanismos de proliferación celular de adhesión se ha realizado a través de la microbalanza de cristal de cuarzo (QCM y espectroscopía de impedancia electroquímica (EIS. La velocidad de adhesión de los osteoblastos sobre la superficie de Ti obtenida a través de medidas con la QCM, sigue una reacción de primer orden, con k=2×10−3 min−1. Los ensayos de impedancia indican que, en ausencia de las células, la resistencia del Ti disminuye con el tiempo (7 días, debido a la presencia de aminoácidos y proteínas del medio de cultivo que se han adsorbido, mientras que en presencia de células, esta disminución es mucho mayor debido a los productos metabólicos generados por las células que aceleran la disolución del Ti.

  1. Computed tomography of the soft tissues of the shoulder. Pt. 3. Calcifying tendinitis of the rotator cuff

    Energy Technology Data Exchange (ETDEWEB)

    Dihlmann, W.; Bandick, J.

    1988-01-01

    Computed tomography of the soft tissue of the shoulder in cases of calcifying tendinitis of the rotator cuff provides the following information: 1. Localisation of the calcium deposits within the rotator cuff. 2. Contours and density of the calcium deposits correlated with the clinical findings as described by Uhthoff et al. Ill-defined contours and non-homogeneous deposits are associated with more severe clinical features. 3. Computed tomography shows that apatite particles, which are not visible radiologically, may penetrate into the shoulder joint and produce synovitis with an effusion. This is of importance in local therapy.

  2. FAP Group Supervision: Reporting Educational Experiences at the University of Sao Paulo, Brazil

    Science.gov (United States)

    Wielenska, Regina Christina; Oshiro, Claudia Kami Bastos

    2012-01-01

    The present article describes and analyzes educational experiences related to the teaching of FAP for psychology graduate students and psychiatry residents at the University of Sao Paulo. The first experience involved psychology graduate students and includes an example of the shaping process occurring within the supervisor-supervisee…

  3. Statistical annual by energy in the municipalities of Sao Paulo State, Brazil - 2006

    International Nuclear Information System (INIS)

    2008-01-01

    This document has as objective the compilation and dissemination of the consumption data corresponding of the main energetics used in the municipalities of Sao Paulo State, Brazil in the year of 2006. The considered energetics are: electric energy, natural gas, petroleum derivatives; hydrated alcohol and biodiesel

  4. Association between dental pulp stones and calcifying nanoparticles.

    Science.gov (United States)

    Zeng, Jinfeng; Yang, Fang; Zhang, Wei; Gong, Qimei; Du, Yu; Ling, Junqi

    2011-01-07

    The etiology of dental pulp stones, one type of extraskeletal calcification disease, remains elusive to date. Calcifying nanoparticles (CNPs), formerly referred to as nanobacteria, were reported to be one etiological factor in a number of extraskeletal calcification diseases. We hypothesized that CNPs are involved in the calcification of the dental pulp tissue, and therefore investigated the link between CNPs and dental pulp stones. Sixty-five freshly collected dental pulp stones, each from a different patient, were analyzed. Thirteen of the pulp stones were examined for the existence of CNPs in situ by immunohistochemical staining (IHS), indirect immunofluorescence staining (IIFS), and transmission electron microscope (TEM). The remaining 52 pulp stones were used for isolation and cultivation of CNPs; the cultured CNPs were identified and confirmed via their shape and growth characteristics. Among the dental pulp stones examined in situ, 84.6% of the tissue samples staines positive for CNPs antigen by IHS; the corresponding rate by IIFS was 92.3 %. In 88.2% of the cultured samples, CNPs were isolated and cultivated successfully. The CNPs were visible under TEM as 200-400 nm diameter spherical particles surrounded by a compact crust. CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp.

  5. The clinical and radiological consideration of calcifying odontogenic cyst of the jaw

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Hae Rym; Kim, Kee Deog; Park, Chang Seo [Dept. of Dental radiology, College of Dentistry, Yensei University, (Korea, Republic of)

    1996-08-15

    Nine cases presented with a calcifying odontogenic cyst to the Dental Hospital of the College of Dentistry, Yonsei University, from January 1987 to September June, 1996, Clinical or histopathological findings were observed according to each radiologic criteria. The results obtained are as follows:1. The male to female ratio was 2 : 1 with a mean age of 25 years. 2. The radiographic appearances of nine caes were well-defined radiolucent lesions in which eight cases were shown until ocular lesions and only one lesion was seen multilocular lesion. 3. Radiologically, three of nine cases were pure radiolucent lesion and others, six cases were radiolucent contained a variable amount of radiopaque material. 4. Histologically, two cases were classified simple cyst, five were cyst associated odontoma, two were neoplastic type. 5. Histological findings according to the radiological classification, two of three radiolucent lesions were simple cys ts; another was a cyst associated with odontoma. While, four of six radiologic mixed lesions turned out to be a cyst associated with odontoma, two was the neoplastic type.

  6. First shark from the Late Devonian (Frasnian) Gogo Formation, Western Australia sheds new light on the development of tessellated calcified cartilage.

    Science.gov (United States)

    Long, John A; Burrow, Carole J; Ginter, Michal; Maisey, John G; Trinajstic, Kate M; Coates, Michael I; Young, Gavin C; Senden, Tim J

    2015-01-01

    Living gnathostomes (jawed vertebrates) comprise two divisions, Chondrichthyes (cartilaginous fishes, including euchondrichthyans with prismatic calcified cartilage, and extinct stem chondrichthyans) and Osteichthyes (bony fishes including tetrapods). Most of the early chondrichthyan ('shark') record is based upon isolated teeth, spines, and scales, with the oldest articulated sharks that exhibit major diagnostic characters of the group--prismatic calcified cartilage and pelvic claspers in males--being from the latest Devonian, c. 360 Mya. This paucity of information about early chondrichthyan anatomy is mainly due to their lack of endoskeletal bone and consequent low preservation potential. Here we present new data from the first well-preserved chondrichthyan fossil from the early Late Devonian (ca. 380-384 Mya) Gogo Formation Lägerstatte of Western Australia. The specimen is the first Devonian shark body fossil to be acid-prepared, revealing the endoskeletal elements as three-dimensional undistorted units: Meckel's cartilages, nasal, ceratohyal, basibranchial and possible epibranchial cartilages, plus left and right scapulocoracoids, as well as teeth and scales. This unique specimen is assigned to Gogoselachus lynnbeazleyae n. gen. n. sp. The Meckel's cartilages show a jaw articulation surface dominated by an expansive cotylus, and a small mandibular knob, an unusual condition for chondrichthyans. The scapulocoracoid of the new specimen shows evidence of two pectoral fin basal articulation facets, differing from the standard condition for early gnathostomes which have either one or three articulations. The tooth structure is intermediate between the 'primitive' ctenacanthiform and symmoriiform condition, and more derived forms with a euselachian-type base. Of special interest is the highly distinctive type of calcified cartilage forming the endoskeleton, comprising multiple layers of nonprismatic subpolygonal tesserae separated by a cellular matrix, interpreted

  7. First shark from the Late Devonian (Frasnian Gogo Formation, Western Australia sheds new light on the development of tessellated calcified cartilage.

    Directory of Open Access Journals (Sweden)

    John A Long

    Full Text Available Living gnathostomes (jawed vertebrates comprise two divisions, Chondrichthyes (cartilaginous fishes, including euchondrichthyans with prismatic calcified cartilage, and extinct stem chondrichthyans and Osteichthyes (bony fishes including tetrapods. Most of the early chondrichthyan ('shark' record is based upon isolated teeth, spines, and scales, with the oldest articulated sharks that exhibit major diagnostic characters of the group--prismatic calcified cartilage and pelvic claspers in males--being from the latest Devonian, c. 360 Mya. This paucity of information about early chondrichthyan anatomy is mainly due to their lack of endoskeletal bone and consequent low preservation potential.Here we present new data from the first well-preserved chondrichthyan fossil from the early Late Devonian (ca. 380-384 Mya Gogo Formation Lägerstatte of Western Australia. The specimen is the first Devonian shark body fossil to be acid-prepared, revealing the endoskeletal elements as three-dimensional undistorted units: Meckel's cartilages, nasal, ceratohyal, basibranchial and possible epibranchial cartilages, plus left and right scapulocoracoids, as well as teeth and scales. This unique specimen is assigned to Gogoselachus lynnbeazleyae n. gen. n. sp.The Meckel's cartilages show a jaw articulation surface dominated by an expansive cotylus, and a small mandibular knob, an unusual condition for chondrichthyans. The scapulocoracoid of the new specimen shows evidence of two pectoral fin basal articulation facets, differing from the standard condition for early gnathostomes which have either one or three articulations. The tooth structure is intermediate between the 'primitive' ctenacanthiform and symmoriiform condition, and more derived forms with a euselachian-type base. Of special interest is the highly distinctive type of calcified cartilage forming the endoskeleton, comprising multiple layers of nonprismatic subpolygonal tesserae separated by a cellular matrix

  8. Biomonitoring of the atmospheric pollution using lichens in the metropolitan area of Sao Paulo city, Brazil

    International Nuclear Information System (INIS)

    Saiki, M.; Fuga, A.; Alves, E.R.; Vasconcellos, M.B.A.; Marcelli, M.P.

    2007-01-01

    The atmospheric pollution of Sao Paulo city is a serious problem due to the expansion of industrial area, increasing number of vehicles and population density. This work presents results obtained in the analysis of lichens collected in different sites of Sao Paulo city and in non-polluted areas of Atlantic Forest. Concentrations of twenty elements were determined in Canoparmelia texanaspecies and comparisons were made between the results obtained in lichens from different sites. High concentrations of the elements As, Ba, Cd, Co, Cr, Fe, La, Mn, Sb and Zn were found for samples collected in sites located near industries and petrochemical plant. Br and Sb concentrations were also high in lichens from sites affected by vehicular emissions. (author)

  9. A Tortuous Process of Surgical Treatment for a Large Calcified Chronic Subdural Hematoma.

    Science.gov (United States)

    Li, Huan; Mao, Xiang; Tao, Xiao-Gang; Li, Jing-Sheng; Liu, Bai-Yun; Wu, Zhen

    2017-12-01

    Calcified chronic subdural hematoma (CCSDH) is a rare disease for which no standard approach to treatment has been established. Reports covering both burr hole trepanation and craniotomy for CCSDH are rare. Furthermore, infection of CCSDH after the burr hole trepanation has not been reported in the literature. A 61-year-old man presented with left frontotemporoparietal CCSDH demonstrated on computed tomography (CT) scan. The patient underwent 2 separate burr hole trepanations with intraoperative irrigation and postoperative drainage. These procedures led to infection of the CCSDH. The patient eventually underwent an open craniotomy to provide complete removal of the hematoma. Owing to the complex contents of a CCSDH, burr hole trepanation cannot adequately drain the hematoma or relieve the mass effect. Craniotomy is a much more reliable approach for achieving complete resection of a CCSDH. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Historical analysis of the oil leakage in the Sao Sebastiao channel, state of Sao Paulo, Brazil; Analise historica dos vazamentos de oleo no canal de Sao Sebastiao, SP

    Energy Technology Data Exchange (ETDEWEB)

    Poffo, Iris Regina F.; Xavier, Jose Carlos de M.; Serpa, Ricardo Rodrigues [CETESB, Sao Paulo, SP (Brazil); Schaeffer-Novelli, Yara [Sao Paulo Univ., SP (Brazil). Inst. de Quimica

    2000-07-01

    Preventing accidents is better than remedying. In order to establish this concept a study was conducted in order to give information about and to prevent sea oil spill accidents. Its first part addressed the main causes of 220 occurrences in north coast of State of Sao Paulo, Brazil based on both CETESB - Companhia de Tecnologia de Saneamento Ambiental and PETROBRAS - Petroleo Brasileiro S. A. data banks. These causes were classified in source, type and mode of failure. In the second part a methodology was developed in order to classify consequences from these events based on two environmental damage indexes regarding ecological and social-economic aspects. Shipping accidents and pipeline failures were responsible for major damages. The most often events caused by operational and mechanical failures mainly in ships and pier were responsible for medium and low severities. (author)

  11. Successful Rotational Atherectomy for an Angulated Calcified Lesion in an Anomalous Right Coronary Artery Using the “Mother-and-Child” Technique

    Directory of Open Access Journals (Sweden)

    Manabu Ogita

    2018-01-01

    Full Text Available Percutaneous coronary intervention (PCI involving the anomalous coronary artery is challenging with respect to difficulty in achieving stable catheterization. Rotational atherectomy (RA can facilitate severely calcified lesions to improve stent delivery and stent expansion; however, its utility in tortuous and angulated coronary arteries is limited with difficulty in delivery of the RA burr. The mother-and-child technique is effective for complex PCIs with increased backup force for device delivery in such complicated cases. We report a case of successful rotational atherectomy using the “mother-and-child” technique with a Dio thrombus aspiration catheter for an angulated calcified lesion in an anomalous origin of the right coronary artery.

  12. Sea anemones may thrive in a high CO2 world.

    Science.gov (United States)

    Suggett, David J; Hall-Spencer, Jason M; Rodolfo-Metalpa, Riccardo; Boatman, Toby G; Payton, Ross; Tye Pettay, D; Johnson, Vivienne R; Warner, Mark E; Lawson, Tracy

    2012-10-01

    Increased seawater pCO 2 , and in turn 'ocean acidification' (OA), is predicted to profoundly impact marine ecosystem diversity and function this century. Much research has already focussed on calcifying reef-forming corals (Class: Anthozoa) that appear particularly susceptible to OA via reduced net calcification. However, here we show that OA-like conditions can simultaneously enhance the ecological success of non-calcifying anthozoans, which not only play key ecological and biogeochemical roles in present day benthic ecosystems but also represent a model organism should calcifying anthozoans exist as less calcified (soft-bodied) forms in future oceans. Increased growth (abundance and size) of the sea anemone (Anemonia viridis) population was observed along a natural CO 2 gradient at Vulcano, Italy. Both gross photosynthesis (P G ) and respiration (R) increased with pCO 2 indicating that the increased growth was, at least in part, fuelled by bottom up (CO 2 stimulation) of metabolism. The increase of P G outweighed that of R and the genetic identity of the symbiotic microalgae (Symbiodinium spp.) remained unchanged (type A19) suggesting proximity to the vent site relieved CO 2 limitation of the anemones' symbiotic microalgal population. Our observations of enhanced productivity with pCO 2 , which are consistent with previous reports for some calcifying corals, convey an increase in fitness that may enable non-calcifying anthozoans to thrive in future environments, i.e. higher seawater pCO 2 . Understanding how CO 2 -enhanced productivity of non- (and less-) calcifying anthozoans applies more widely to tropical ecosystems is a priority where such organisms can dominate benthic ecosystems, in particular following localized anthropogenic stress. © 2012 Blackwell Publishing Ltd.

  13. Rotational atherectomy before paclitaxel-eluting stent implantation in complex calcified coronary lesions: Two-year clinical outcome of the randomized ROTAXUS trial.

    Science.gov (United States)

    de Waha, Suzanne; Allali, Abdelhakim; Büttner, Heinz-Joachim; Toelg, Ralph; Geist, Volker; Neumann, Franz-Josef; Khattab, Ahmed A; Richardt, Gert; Abdel-Wahab, Mohamed

    2016-03-01

    In the randomized ROTAXUS trial, routine lesion preparation of complex calcified coronary lesions using rotational atherectomy (RA) prior to paclitaxel-eluting stent implantation did not reduce the primary endpoint of angiographic late lumen loss at 9 months compared to stenting without RA. So far, no long-term data of prospective head-to-head comparisons between both treatment strategies have been reported. ROTAXUS randomly assigned patients with complex calcified coronary lesions to RA followed by stenting (n = 120) or stenting without RA (n = 120). The primary endpoint of the current analysis was the occurrence of major adverse cardiac events (MACE) at 2-year follow-up defined as the composite of death, myocardial infarction, and target vessel revascularization (TVR). At 2 years, MACE occurred in 32 patients in the RA group and 37 patients in the standard therapy group (29.4% vs. 34.3%, P = 0.47). The rates of death (8.3% vs. 7.4%, P = 1.00), myocardial infarction (8.3% vs. 6.5%, P = 0.80), target lesion revascularization (TLR, 13.8% vs. 16.7%, P = 0.58), and TVR (19.3% vs. 22.2%, P = 0.62) were similar in both groups. Despite high rates of initial angiographic success, nearly one third of patients enrolled in ROTAXUS experienced MACE within 2-year follow-up, with no differences between patients treated with or without RA. © 2015 Wiley Periodicals, Inc.

  14. Radiogeologic reconnaissance in Perus region, Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Coelho, P.E.F.P.

    1980-01-01

    The determination of the means and the identification of the radiometric anomalies, and the classification of this anomalies orgin, and it probable correlation with uraniferous secundary minerals from Perus and Taipas were studied. Petrological, stratigraphic, tectonic and geochronological studies in this region were done. The radiometric anomalies was observed in granitic-pegmatic massifs, later Sao Roque group. The radiocitivity of this area was affected by litology, being the pegmatite more radiogenic than granite. The topography is also a influent factor to detected radioactivity. The cinthylometry was efficient to U an Th anomalies detection. Three kinds of radioctive focus were detected by microautoradiography method [pt

  15. Feasibility study on energy conservation at Sao Paulo state primary schools in the Federative Republic of Brazil

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A feasibility study has been performed on the energy conservation project aimed at the future CDM in relation with energy consumption in the lighting equipment at the Sao Paulo state primary schools in the Federative Republic of Brazil. Out of about 5,500 primary schools existing in Sao Paulo, 3,414 schools were selected as the objects of the surveys and discussions. The present program intends to introduce energy saving facilities such as high-efficiency fluorescent lamps and controls for illuminating the primary schools to save the energies and reduce emission of the global warming gases. In this economy project, the owners do not need to make initial investments, wherein the required funds are taken care by conservation of electric power during the service contract period. If about 3,000 schools are selected as the object, a fund of about 2.3 billion yen will be required in total. Total energy conservation quantity during 15 years from 2002 to 2016 as achieved by this project will be 532,740 MWh, corresponding to 114,540 tons of crude oil. The effect of reducing the emission of global warming gases will be 110,949 t-CO2 during the same period of time. (NEDO)

  16. The response of calcifying plankton to climate change in the Pliocene

    Directory of Open Access Journals (Sweden)

    C. V. Davis

    2013-09-01

    Full Text Available As a result of anthropogenic pCO2 increases, future oceans are growing warmer and lower in pH and oxygen, conditions that are likely to impact planktic communities. Past intervals of elevated and changing pCO2 and temperatures can offer a glimpse into the response of marine calcifying plankton to changes in surface oceans under conditions similar to those projected for the future. Here we present new records of planktic foraminiferal and coccolith calcification (weight and size from Deep Sea Drilling Project Site 607 (mid-North Atlantic and Ocean Drilling Program Site 999 (Caribbean Sea from the Pliocene, the last time that pCO2 was similar to today, and extending through a global cooling event into the intensification of Northern Hemisphere glaciation (3.3 to 2.6 million years ago. Test weights of both surface-dwelling Foraminifera Globigerina bulloides and thermocline-dwelling Foraminifera Globorotalia puncticulata vary with a potential link to regional temperature variation in the North Atlantic, whereas in the tropics Globigerinoides ruber test weight remains stable. In contrast, reticulofenestrid coccoliths show a narrowing size range and a decline in the largest lith diameters over this interval. Our results suggest no major changes in plankton calcite production during the high pCO2 Pliocene or during the transition into an icehouse world.

  17. Characterization of the brazilian alcohol program in the State of Sao Paulo: production costs to plants and distillery homogeneous groups

    International Nuclear Information System (INIS)

    Miyake, M.Y.; Chung, S.L.; Telhada, M.A.L.

    1987-01-01

    The ethanol producing plants in the State of Sao Paulo present variation in scale of production, productivity and cost structure. This paper presents production costs and analyses the competitiveness of neat ethanol with respect to gasoline, for each of the eight homogeneous groups of distilleries in operation in the State of Sao Paulo. The study shows that, at prices of march 1986, there is a variation of operational costs in the range of 1.46 to 1.95 cruzados per liter of hydrous ethanol. The reference price, including operational costs and interest on capital, varies from 2.47 to 3.15 cruzados per liter. These prices do not consider subsidies given by the Government under the Brazilian National Alcohol Program. The price of hydrous ethanol in Barrel Equivalent of Oil goes from 33.3 to 43.1 american dollars, showing that ethanol is not currently competitive with gasoline. (author)

  18. Calcified subdural haematomas associated with arrested hydrocephalus - late sequelae of shunt operation in infancy

    Energy Technology Data Exchange (ETDEWEB)

    Barmeir, E.P.; Stern, D.; Harel, S.; Holtzman M.; Krije, T.J.

    1985-08-01

    Calcified chronic subdural haematoms (SDH) and features of arrested (compensated) hydrocephalus were demonstrated by skull radiography and cranial computed tomography (CT) in two children who had no neurological deficit. Ventricular surgical drainage had been performed 8 and 11 years prior to admission and the haematomas remained subsequently undetected. The following presentation will serve to illustrate the characteristic radiological features of this entity, the issue of management, and includes a review of the literature.

  19. SEISMIC STUDY OF THE AGUA DE PAU GEOTHERMAL PROSPECT, SAO MIGUEL, AZORES.

    Science.gov (United States)

    Dawson, Phillip B.; Rodrigues da Silva, Antonio; Iyer, H.M.; Evans, John R.

    1985-01-01

    A 16 station array was operated over the 200 km**2 central portion of Sao Miguel utilizing 8 permanent Instituto Nacional de Meterologia e Geofisica stations and 8 USGS portable stations. Forty four local events with well constrained solutions and 15 regional events were located. In addition, hundreds of unlocatable seismic events were recorded. The most interesting seismic activity occurred in a swarm on September 6 and 7, 1983 when over 200 events were recorded in a 16 hour period. The seismic activity around Agua de Pau was centered on the east and northeast slopes of the volcano. The data suggest a boiling hydrothermal system beneath the Agua de Pau volcano, consistent with a variety of other data.

  20. Neoproterozoic alkaline magmatism in Ilha do Cardoso, southeastern coast of Sao Paulo State, Brazil

    International Nuclear Information System (INIS)

    Weber, Werner; Basei, Miguel A.S.; Siga Junior, Oswaldo; Sato, Kei

    2001-01-01

    This work focuses on the geology and geochronology of rocks cropping out on Cardoso Island, on the southeastern coast of Sao Paulo State, close to the boundary with Parana State. The island, with an area of about 151 km 2 is a protected area administered by the Forest Institute of the Secretariat for the Environment of the State of Sao Paulo. It is mountainous, with a peak at 814 m, and is covered by dense Atlantic Forest vegetation. The island is made up mainly of an igneous complex with light grey leucocratic, inequigranular, medium to coarse-grained syenites. The Tres Irmaos Syenite (STI), composed of pyroxene, hornblende, and perthitic to mesoperthitic microcline, predominates has magmatic flow structures, and it cut by the pinkish grey, leucocratic medium-grained Cambriu alkali-feldspar granites (GC). Geochemical analysis of STI and GC demonstrate their metaluminous alkaline nature and late orogenic to anorogenic character. The bodies formed between 620 and 570 Ma according to U-Pb dating of zircons and cooled between 597 and 531 Ma (K-Ar in amphiboles). Whole rock Sm-Nd analyses yield Meso- and Paleoproterozoic TDM ages (1,500 - 2,200 Ma). A belt of low-grade metasedimentary rocks occurs in the northern part of the island. Quartz schist, quartz-mica schist and mica-quartz schist, often-containing andalusite and cordierite, predominate. Geochemical and geochronological data suggest that the sources of the metasediments were continental arc andesites of whose protoliths separated from the mantle between 1,800 and 2,200 Ma during the Paleoproterozoic. These metasediments probably continue on the continent in the Taquari region and extend southwards in narrow strips between the granitoids of the Paranagua Domain. (author)

  1. Studies of osteoporosis in urban residents of Sao Paulo, Brazil, using isotope related techniques

    International Nuclear Information System (INIS)

    Borelli, A.

    2000-01-01

    This project is aimed at defining the peak of bone mass in an urban population chosen randomly on account of the great miscegenation in Sao Paulo. It is quite important to know the peak of bone mass and the factors that would interfere in the amount of bone formed as they are valuable guides for future plans of osteoporosis prevention. In our previous meeting it was decided to increase the number of individuals studied from 210 to 350 and proceed with the evaluation of patient information according with the questionnaire proposed. In this report it is presented the results on bone mineral density of lumbar L1-L4 and neck of femur in the total of 350 patients (175 women and 175 men). The complete data, as well as the information obtained from the questionnaire will be sent in the future reports as programed in the meeting of Sao Paulo

  2. Possible use of calcifying nanoparticles in immature root apex treatment

    Directory of Open Access Journals (Sweden)

    Mohammed S Alenazy

    2014-01-01

    Full Text Available Introduction: There are reports in the literature, which describe different techniques and materials in the challenging management of thin dentin walls and immature root apex. It has been suggested that calcifying nanoparticles (CNPs could be used in the management of these conditions. The Hypothesis: Compositionally modified CNPs made into a paste could become efficacious in managing thin dentin walls and immature root apex. Calcium and phosphate ions when mixed with CNPs could form a synthetic nanopaste that clinicians could use to manage thin dentin walls and to get a biological seal for immature root apex. Evaluation of the Hypothesis: CNPs can replicate and could facilitate the aggregations of calcium hydroxyapatite to produce a self-surrounding shell. These characteristics of CNPs could be used through their biomineralization process as initial nidus of calcification for further calcification progression to achieve total biological apical seal. If the hypothesis could be supported by biomineralization behavior of the paste (CNPs, Ca2 + , and PO4− , a new therapeutic agent would have been added to the armamentarium of endodontists. There is need for more in vivo and in vitro investigations of modified nanopaste to manage these conditions.

  3. Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation.

    Science.gov (United States)

    Núñez-Toldrà, Raquel; Martínez-Sarrà, Ester; Gil-Recio, Carlos; Carrasco, Miguel Ángel; Al Madhoun, Ashraf; Montori, Sheyla; Atari, Maher

    2017-04-21

    Biomaterials are widely used to regenerate or substitute bone tissue. In order to evaluate their potential use for clinical applications, these need to be tested and evaluated in vitro with cell culture models. Frequently, immortalized osteoblastic cell lines are used in these studies. However, their uncontrolled proliferation rate, phenotypic changes or aberrations in mitotic processes limits their use in long-term investigations. Recently, we described a new pluripotent-like subpopulation of dental pulp stem cells derived from the third molars (DPPSC) that shows genetic stability and shares some pluripotent characteristics with embryonic stem cells. In this study we aim to describe the use of DPPSC to test biomaterials, since we believe that the biomaterial cues will be more critical in order to enhance the differentiation of pluripotent stem cells. The capacity of DPPSC to differentiate into osteogenic lineage was compared with human sarcoma osteogenic cell line (SAOS-2). Collagen and titanium were used to assess the cell behavior in commonly used biomaterials. The analyses were performed by flow cytometry, alkaline phosphatase and mineralization stains, RT-PCR, immunohistochemistry, scanning electron microscopy, Western blot and enzymatic activity. Moreover, the genetic stability was evaluated and compared before and after differentiation by short-comparative genomic hybridization (sCGH). DPPSC showed excellent differentiation into osteogenic lineages expressing bone-related markers similar to SAOS-2. When cells were cultured on biomaterials, DPPSC showed higher initial adhesion levels. Nevertheless, their osteogenic differentiation showed similar trend among both cell types. Interestingly, only DPPSC maintained a normal chromosomal dosage before and after differentiation on 2D monolayer and on biomaterials. Taken together, these results promote the use of DPPSC as a new pluripotent-like cell model to evaluate the biocompatibility and the differentiation

  4. Perspectives for distributed generation of electricity in the States of Sao Paulo, Bahia and Mato Grosso; Perspectivas da geracao distribuida de eletricidade nos estados de Sao Paulo, Bahia e Mato Grosso

    Energy Technology Data Exchange (ETDEWEB)

    Bajay, Sergio Valdir; Leite, Alvaro Afonso Furtado [Universidade Estadual de Campinas (NIPE/UNICAMP), SP (Brazil). Nucleo Interdisciplinar de Planejamento Energetico], Email: bajay@fem.unicamp.br; Carvalho, Claudio Bezerra de [Universidade do Estado da Bahia (UNEB), Alagoinhas, BA (Brazil); Dorileo, Ivo Leandro [Universidade Federal do Mato Grosso (UFMT), Cuiaba, MT (Brazil). Nucleo Interdisciplinar de Estudos em Planejamento Energetico

    2006-07-01

    This paper addresses the concept of distributed generation of electricity and the current support policies for such kind of generation in the country. Their diffusion perspectives in the States of Sao Paulo, Bahia and Mato Grosso are discussed. The more promising technologies and new policies for them are pointed out. (author)

  5. Effective endovascular treatment of calcified femoropopliteal disease with directional atherectomy and distal embolic protection: final results of the DEFINITIVE Ca⁺⁺ trial.

    Science.gov (United States)

    Roberts, David; Niazi, Khusrow; Miller, William; Krishnan, Prakash; Gammon, Roger; Schreiber, Theodore; Shammas, Nicolas W; Clair, Daniel

    2014-08-01

    The purpose of the DEFINITIVE Ca(++) study was to evaluate the safety and effectiveness of directional atherectomy and distal embolic protection, used together to treat moderate to severely calcified femoropopliteal lesions. Despite advances in endovascular treatment modalities, treatment of calcified lesions remains a challenge. A total of 133 subjects with 168 moderate to severely calcified lesions were enrolled. Lesions were treated with directional atherectomy devices, coupled with distal embolic protection. The 30-day freedom from MAE rate was 93.1%. Per angiographic core laboratory assessment, the primary effectiveness endpoint (≤50% residual diameter stenosis) was achieved in 92.0% (lower confidence bound of 87.6%) of lesions. By core lab analysis, these results did not achieve the success criteria (90%) for the primary effectiveness objective. Per site assessment, the objective was met with the endpoint being achieved in 97.0% (lower confidence bound 93.8%). A mean residual diameter stenosis of 33.3% was achieved with the directional atherectomy device. This was further decreased to 24.1% with the use of adjunctive therapy. The proportion of asymptomatic subjects [Rutherford Clinical Category (RCC) = 0] increased from 0% at baseline to 52.3% at the 30-day follow-up visit. In total, 88.5% of subjects experienced an improvement of one or more Rutherford categories. The results of the DEFINITIVE Ca++ study demonstrate that the SilverHawk and TurboHawk atherectomy devices are safe and effective in the endovascular treatment of moderate to severely calcified lesions in the superficial femoral and/or popliteal arteries when used with the SpiderFX distal embolic protection device. © 2014 The Authors. Catheterization and Cardiovascular Interventions. Published by Wiley Periodicals, Inc.

  6. Characterization of the PROALCOOL in the State of Sao Paulo:, Brazil establishment of homogeneous groups of plants and distilleries; Caracterizacao do PROALCOOL no Estado de Sao Paulo: formacao de grupos homogeneos de usinas e destilarias

    Energy Technology Data Exchange (ETDEWEB)

    Shih, L.C.; Hwa, C.M.F.; Andreoni, S.; Myiake, M.Y. [Instituto de Pesquisas Tecnologicas (IPT), Sao Paulo, SP (Brazil)

    1987-12-31

    A methodology to stratify the ethanol plants operating in the State of Sao Paulo, Brazil, in homogeneous groups is presented. This group was obtained by multivariate stratification techniques considering factors as scale of production, agricultural productivity, industrial efficiency and effective industrial capacity utilization. This methodology differs from those adopted by the Fundacao Getulio Vargas and the Organizacao dos Plantadores de Cana-Orplana who consider only one variable- the scale of production. The advantage of this multivariate stratification is that the result clusters reflect more accurately the differences among the existing distilleries. 4 refs., 2 tabs.

  7. A universal carbonate ion effect on stable oxygen isotope ratios in unicellular planktonic calcifying organisms

    OpenAIRE

    Ziveri, P.; Thoms, S.; Probert, I.; Geisen, M.; Langer, H.

    2012-01-01

    The oxygen isotopic composition (δ18O) of calcium carbonate of planktonic calcifying organisms is a key tool for reconstructing both past seawater temperature and salinity. The calibration of paloeceanographic proxies relies in general on empirical relationships derived from field experiments on extant species. Laboratory experiments have more often than not revealed that variables other than the target parameter influence the proxy signal, which makes proxy c...

  8. Calcium homeostasis and vitamin D metabolism and expression in strongly calcifying laying birds.

    Science.gov (United States)

    Bar, Arie

    2008-12-01

    Egg laying and shell calcification impose severe extra demands on ionic calcium (Ca2+) homeostasis; especially in birds characterized by their long clutches (series of eggs laid sequentially before a "pause day"). These demands induce vitamin D metabolism and expression. The metabolism of vitamin D is also altered indirectly, by other processes associated with increased demands for calcium, such as growth, bone formation and egg production. A series of intestinal, renal or bone proteins are consequently expressed in the target organs via mechanisms involving a vitamin D receptor. Some of these proteins (carbonic anhydrase, calbindin and calcium-ATPase) are also found in the uterus (eggshell gland) or are believed to be involved in calcium transport in the intestine or kidney (calcium channels). The present review deals with vitamin D metabolism and the expression of the above-mentioned proteins in birds, with special attention to the strongly calcifying laying bird.

  9. Emerging Futures for Sao Tome & Principe’s International Airport Campus

    Science.gov (United States)

    2007-12-03

    from Illinois State University and a Culinary Arts degree from New England Culinary Institute. He is currently a research assistallt with the Land...Director. We would also like to thank the College of Fine and Applied Arts and the East St. Louis Action Research Project for their financial contributions...airportS. Reliance on tourism carries risks to the citizens of Sao Tome in the form of cheapening customs and traditions and adaptation of local arts

  10. Energy balance of Sao Paulo state, Brazil 2008. Calendar year 2007

    International Nuclear Information System (INIS)

    2008-01-01

    This document presents the consolidated annual energy balances and also tables and graphics which detailed the evolution and the present status of offer and demand of each energy resources used by Sao Paulo, Brazil, economy and regionalized and environmental information. It covers the period of 1994 to 2007 and includes energy fluxes of the energy primary and secondary resources, from the production to the final consumption of the main sectors of the state economy

  11. Energy balance of Sao Paulo state, Brazil 2009. Calendar year 2008

    International Nuclear Information System (INIS)

    2009-01-01

    This document presents the consolidated annual energy balances and also tables and graphics which detailed the evolution and the present status of offer and demand of each energy resources used by Sao Paulo, Brazil, economy and regional and environmental information. It covers the period of 1995 to 2008 and includes energy fluxes of the energy primary and secondary resources, from the production to the final consumption of the main sectors of the state economy

  12. Acquisition of anoikis resistance in human osteosarcoma cells does not alter sensitivity to chemotherapeutic agents

    International Nuclear Information System (INIS)

    Díaz-Montero, C Marcela; McIntyre, Bradley W

    2005-01-01

    Chemotherapy-induced cell death can involve the induction of apoptosis. Thus, aberrant function of the pathways involved might result in chemoresistance. Since cell adhesion to the extracellular matrix acts as a survival factor that homeostatically maintains normal tissue architecture, it was tested whether acquisition of resistance to deadhesion-induced apoptosis (anoikis) in human osteosarcoma would result in resistance to chemotherapy. Osteosarcoma cell lines (SAOS-2 and TE-85) obtained from ATCC and were maintained in complete Eagle's MEM medium. Suspension culture was established by placing cells in tissue culture wells coated with poly-HEMA. Cell cytotoxicity was determined using a live/dead cytotoxicity assay. Cell cycle/apoptosis analyses were performed using propidium iodide (PI) staining with subsequent FACS analysis. Apoptosis was also assayed by Annexin-FITC/PI staining. Etoposide, adriamycin, vinblastine, cisplatin and paclitaxel were able to induce apoptosis in human osteosarcoma cells SAOS-2 regardless of their anoikis resistance phenotype or the culture conditions (adhered vs. suspended). Moreover, suspended anoikis resistant TE-85 cells (TE-85ar) retained their sensitivity to chemotherapy as well. Acquisition of anoikis resistance in human osteosarcoma cells does not result in a generalized resistance to all apoptotic stimuli, including chemotherapy. Moreover, our results suggest that the pathways regulating anoikis resistance and chemotherapy resistance might involve the action of different mediators

  13. Acquisition of anoikis resistance in human osteosarcoma cells does not alter sensitivity to chemotherapeutic agents

    Directory of Open Access Journals (Sweden)

    McIntyre Bradley W

    2005-04-01

    Full Text Available Abstract Background Chemotherapy-induced cell death can involve the induction of apoptosis. Thus, aberrant function of the pathways involved might result in chemoresistance. Since cell adhesion to the extracellular matrix acts as a survival factor that homeostatically maintains normal tissue architecture, it was tested whether acquisition of resistance to deadhesion-induced apoptosis (anoikis in human osteosarcoma would result in resistance to chemotherapy. Methods Osteosarcoma cell lines (SAOS-2 and TE-85 obtained from ATCC and were maintained in complete Eagle's MEM medium. Suspension culture was established by placing cells in tissue culture wells coated with poly-HEMA. Cell cytotoxicity was determined using a live/dead cytotoxicity assay. Cell cycle/apoptosis analyses were performed using propidium iodide (PI staining with subsequent FACS analysis. Apoptosis was also assayed by Annexin-FITC/PI staining. Results Etoposide, adriamycin, vinblastine, cisplatin and paclitaxel were able to induce apoptosis in human osteosarcoma cells SAOS-2 regardless of their anoikis resistance phenotype or the culture conditions (adhered vs. suspended. Moreover, suspended anoikis resistant TE-85 cells (TE-85ar retained their sensitivity to chemotherapy as well. Conclusion Acquisition of anoikis resistance in human osteosarcoma cells does not result in a generalized resistance to all apoptotic stimuli, including chemotherapy. Moreover, our results suggest that the pathways regulating anoikis resistance and chemotherapy resistance might involve the action of different mediators.

  14. Determination of Cl, K, and Na in the blood serum of healthy elderly in the city of Sao Paulo, SP

    International Nuclear Information System (INIS)

    Araujo, Nathalia Souza de; Saiki, Mitiko

    2009-01-01

    This work determined the blood serum concentration of Cl, K, and Na from a healthy elderly population of the city of Sao Paulo, Sao Paulo, Brazil viewing the comparison with reference values used in clinical laboratories. For collecting these samples the present work were approved by the Ethic Committee of the FMUSP and IPEN-CNEN/SP. The quality of the analytical results were evaluated by the analysis of the reference material certification NIST 1577b Bovine Liver. The results presented good accuracy and precision due to the fact that they presented relative errors less than 5.2 % and standard deviation less than 8.6 %. The average concentrations (mmol L -1 ) of Cl in the analysed serum were 99.1; the K were 3.9 and the Na were 136.9. The obtained data allows the to conclude that the mostly of the elderly presented Cl, K and Na concentrations in the range of reference values used in the clinical laboratories

  15. Petrological, geochemical and isotopic characteristics of lignite and calcified lignite from mining area Pesje, Velenje Basin, Slovenia

    Science.gov (United States)

    Vrabec, Mirijam; Markič, Miloš; Vrabec, Marko; Jaćimović, Radojko; Kanduč, Tjaša

    2014-05-01

    Lignite (organic rich) and calcified lignite (inorganic rich) samples from excavation field -50c mining area Pesje, Velenje Basin, Slovenia were investigated. During geological and structural mapping lignite and calcified lignite samples were systematically taken for determination of their petrological, geochemical and isotopic characteristics. Lignite is composed of fine detritical gelified matrix. At least five different types of calcified lignite were recognized forming laminations, calcifications after wood, petrified wood and complete replacements of lignite with carbonate. All measured parameters so far indicate geochemical processes during sedimentation of the Velenej Basin. After macroscopic description samples were split to organic and inorganic component (Ward, 1984) and powdered in an agate mortar for geochemical and isotopic analyses. Major and trace elements (As, B, Ba, Cd, Co, Cr, Cu, Hg, Mn, Mo, Sb, Se, Th, U, Zn) in these samples were determined by instrumental neutron activation analysis (INAA) using k-0 standardization method (Jaćimović et al, 2002). The isotopic composition of carbon and nitrogen was determined using a Europa 20-20 continuous flow IRMS ANCA-SL preparation module. A 1 mg amount of a sample was weighed in a tin capsule for carbon and 10 mg for nitrogen analysis. Samples for carbon analyses were pretreated with 1 M HCl to remove carbonates. Carbonate samples from carbonate-rich strata and calcified xylite were first roasted at 450 deg C (Krantz et al., 1987). Three miligrams of carbonate sample was transformed into CO2 by reaction with anhydrous H3PO4 at 55 deg C under vacuum (McCrea, 1950) and measured with GV 2003 isotope ratio mass spectrometer. Measured isotopic composition of oxygen as VPDB values was recalculated to the VSMOW reference standard to enable the comparison with data from other coal basins. SEM/EDXS of carbonate rich sediments was performed with JEOL JSM 5800 electron microanalyzer scanning electron microscope

  16. Electron beam application for studies carried out with real domestic sewage from Sao Paulo wastewater treatment plants

    International Nuclear Information System (INIS)

    Borrely, S.I.; Sampa, M.H.O.; Duarte, C.L.

    2001-01-01

    The incompatibility between industrial development and clean environment requires intensive search for waste mitigation technology. Since the aquatic resources have been the most impacted from the environments, improvements on wastewater treatment technologies have been considered. The Nuclear Research Institute has dedicated attention to this problem since 1990. According to the Governmental Sewage Company, SABESP, Sao Paulo Metropolitan Region, RMSP, is treating 18 cubic meters of sewage per second at five stations. The throughput of each station is: ETE Barueri - 9.5 m 3 /s; ETE ABC - 3.0 m 3 /s; ETE Sao Miguel - 1.5 m 3 /s; ETE Parque Novo Mundo - 2.5 m 3 /s and ETE Suzano - 1.5 m 3 /s. Real effluents from the municipalities have been submitted to electron beam accelerator for different purposes, and using batch system. The samplings were composite and the wastewater were irradiated at Pyrex vessels, 246 mL per sample. The radiation doses were defined by current variation and the energy was fixed in 1.4MeV. The conveyor velocity was 6,72 m/min. A Dynamitron EBA, 37,5kW was the electron source

  17. Increasing trends of sleep complaints in the city of Sao Paulo, Brazil.

    Science.gov (United States)

    Santos-Silva, Rogerio; Bittencourt, Lia Rita Azeredo; Pires, Maria Laura Nogueira; de Mello, Marco Tulio; Taddei, Jose Augusto; Benedito-Silva, Ana Amelia; Pompeia, Celine; Tufik, Sergio

    2010-06-01

    The aim of this study was to compare the prevalence of sleep habits and complaints and to estimate the secular trends through three population-based surveys carried out in 1987, 1995, and 2007 in the general adult population of the city of Sao Paulo, Brazil. Surveys were performed using the same three-stage cluster-sampling technique in three consecutive decades to obtain representative samples of the inhabitants of Sao Paulo with respect to gender, age (20-80 years), and socio-economic status. Sample sizes were 1000 volunteers in 1987 and 1995 surveys and 1101 in a 2007 survey. In each survey, the UNIFESP Sleep Questionnaire was administered face-to-face in each household selected. For 1987, 1995, and 2007, respectively, difficulty initiating sleep (weighted frequency %; 95% CI) [(13.9; 11.9-16.2), (19.15; 16.8-21.6), and (25.0; 22.5-27.8)], difficulty maintaining sleep [(15.8; 13.7-18.2), (27.6; 24.9-30.4), and (36.5; 33.5-39.5)], and early morning awakening [(10.6; 8.8-12.7), (14.2; 12.2-16.5), and (26.7; 24-29.6)] increased in the general population over time, mostly in women. Habitual snoring was the most commonly reported complaint across decades and was more prevalent in men. There was no statistically significant difference in snoring complaints between 1987 (21.5; 19.1-24.2) and 1995 (19.0; 16.7-21.6), but a significant increase was noted in 2007 (41.7; 38.6-44.8). Nightmares, bruxism, leg cramps, and somnambulism complaints were significantly higher in 2007 compared to 1987 and 1995. All were more frequent in women. This is the first study comparing sleep complaints in probabilistic population-based samples from the same metropolitan area, using the same methodology across three consecutive decades. Clear trends of increasing sleep complaints were observed, which increased faster between 1995 and 2007 than from 1987 to 1995. These secular trends should be considered a relevant public health issue and support the need for development of health care and

  18. La experiencia del partido dos trabalhadores en la alcadía de Sao Paulo

    Directory of Open Access Journals (Sweden)

    Lúcio KOWARICK

    2009-11-01

    Full Text Available RESUMEN: En este artículo, Lucio Kowarick y André Singer analizan la gestión del Partido dos Trabalhadores en la Alcaldía de Sao Paulo encabezada por Luiza Erundina. Con esta evaluación pretenden conocer la acción del PT a través de uno de sus principales elementos de construcción del PT, el gobierno de las ciudades, que complementa a los otros dos, la vinculación con los movimientos sindicales y populares y la acción parlamentaria.ABSTRACT: In this article, Lucio Kowarick and Andre Singer analyses the government of Sao Paulo by Partido dos Trabalhadores, with Luiza Erundina. With this evaluation, they try to know the action of PT through one of the main elements of contruction of PT, the government of cities. The other are links with trade unions and popular movements and parliamentarian action.

  19. Scientific analysis of a calcified object from a post-medieval burial in Vienna, Austria.

    Science.gov (United States)

    Binder, Michaela; Berner, Margit; Krause, Heike; Kucera, Matthias; Patzak, Beatrix

    2016-09-01

    Calcifications commonly occur in association with soft tissue inflammation. However, they are not often discussed in palaeopathological literature, frequently due to problems of identification and diagnosis. We present a calcified object (40×27×27cm) found with a middle-aged male from a post-medieval cemetery in Vienna. It was not recognized during excavation, thus its anatomical location within the body remains unknown. The object was subject to X-ray, SEM and CT scanning and compared to historic pathological objects held in the collection of the Natural History Museum Vienna. Two of closest resemblance, a thyroid adenoma and goitre were subject to similar analytical techniques for comparison. Despite similarities between all objects, the structure of the object most closely conforms to a thyroid tumor. Nevertheless, due to similar pathophysiological pathways and biochemical composition of calcified soft tissue, a secure identification outside of its anatomical context is not possible. The research further highlights the fact that recognition of such objects during excavation is crucial for a more conclusive diagnosis. Historic medical records indicate that they were common and might therefore be expected to frequently occur in cemeteries. Consequently, an increasing the dataset of calcifications would also aid in extending the knowledge about diseases in past human populations. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Modernization perspectives of the Sao Paulo State sugarcane sector through the clean development mechanism and potential carbon credits generation

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Suani Teixeira; Lora, Beatriz Acquaro [Brazilian Reference Center on Biomass (CENBIO/USP), SP (Brazil)], Emails: suani@iee.usp.br, blora@iee.usp.br

    2009-07-01

    The world-wide necessity of greenhouse gases mitigation and the intergovernmental mobilization to reach the objectives established by the United Nations Framework on Climate Change (UNFCCC) has opened space for the renewable energy increase in the world's energy matrix. In Brazil, the solid sugarcane industry currently develops business in the scope of the clean development mechanism (CDM) under the Kyoto's Protocol, by means of 18 biomass-based projects, with renewable energy generation through bagasse cogeneration at 20 Sao Paulo State's sugarcane production units. The projects activity's consists of increasing the efficiency in the bagasse cogeneration facilities, qualifying the units to sell surplus electricity to the national grid, avoiding the dispatch of the same amount of energy produced by fossil-fuelled thermal plants to that grid. The reduced emissions are measured in carbon equivalent and can be converted into negotiable credits. The objective of this study was to build a 'state of art' scenario, calculating the potential emissions reduction through CDM projects for the sugarcane sector of Sao Paulo State, in which we consider the adherence of all the production units of the State to the CDM projects. The technological parameters used to elaborate the scenario were provided by the Sao Paulo State Government Bioenergy Special Commission and the baseline factor used of 0,268 tCO{sub 2}e/MWh was the adopted by the CDM projects in operation in the State. The sugarcane database for the calculations was the production ranking provided by UNICA for the 2006/2007 season. In the most conservative scenario (40 bar bagasse) 131 units could generate 607 MWm of surplus power avoiding the emission of 1.404.593 tCO{sub 2}e/year. For the 92 bar (bagasse and straw) scenario, the units could generate 3.055 MWm of surplus power avoiding 12.199.443 tCO{sub 2}e/year. (author)

  1. Report of Two Cases of Combined Odontogenic Tumors: Ameloblastoma with Odontogenic Keratocyst and Ameloblastic Fibroma with Calcifying Odontogenic Cyst.

    Science.gov (United States)

    Neuman, Ashley Nicole; Montague, Lindsay; Cohen, Donald; Islam, Nadim; Bhattacharyya, Indraneel

    2015-09-01

    Combined odontogenic neoplasms have rarely been documented. Such tumors have also been described by other researchers as "hybrid" lesions. The histologic features are often identical to other individually well-established odontogenic neoplasms such as ameloblastoma, adenomatoid odontogenic tumor, ameloblastic fibroma (AF), and ameloblastic fibro-odontoma. Their clinical presentation is variable, ranging from cysts to neoplasms showing varying degrees of aggressive behavior. Most combined tumors contain features of one of the odontogenic tumors in combination with either a calcifying odontogenic cyst (COC) or a calcifying epithelial odontogenic tumor. We present two new cases of combined odontogenic tumors: an ameloblastoma with an odontogenic keratocyst and an AF with COC. Predicting clinical outcome is challenging when a combination tumor is encountered due to the paucity of such lesions. One must understand salient features of these entities and differentiate them from the more common conventional neoplasms to expand classification and provide prognostic criteria.

  2. In situ survey of life cycle phases of the coccolithophore Emiliania huxleyi (Haptophyta).

    Science.gov (United States)

    Frada, Miguel J; Bidle, Kay D; Probert, Ian; de Vargas, Colomban

    2012-06-01

    The cosmopolitan coccolithophore Emiliania huxleyi is characterized by a strongly differentiated haplodiplontic life cycle consisting of a diploid phase, generally bearing coccoliths (calcified) but that can be also non-calcified, and a non-calcified biflagellated haploid phase. Given most studies have focused on the bloom-producing calcified phase, there is little-to-no information about non-calcified cells in nature. Using field mesocoms as experimental platforms, we quantitatively surveyed calcified and non-calcified cells using the combined calcareous detection fluorescent in situ hybridization (COD-FISH) method and qualitatively screened for haploid specific transcripts using reverse transcription-PCR during E. huxleyi bloom successions. Diploid, calcified cells formed dense blooms that were followed by the massive proliferation of E. huxleyi viruses (EhVs), which caused bloom demise. Non-calcified cells were also detected throughout the experiment, accounting for a minor fraction of the population but becoming progressively more abundant during mid-late bloom periods concomitant with EhV burst. Non-calcified cell growth also paralleled a distinct window of haploid-specific transcripts and the appearance of autotrophic flagellates morphologically similar to haploid cells, both of which are suggestive of meiosis and sexual life cycling during natural blooms of this prominent marine phytoplankton species. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

  3. Technique optimization of orbital atherectomy in calcified peripheral lesions of the lower extremities: the CONFIRM series, a prospective multicenter registry.

    Science.gov (United States)

    Das, Tony; Mustapha, Jihad; Indes, Jeffrey; Vorhies, Robert; Beasley, Robert; Doshi, Nilesh; Adams, George L

    2014-01-01

    The purpose of CONFIRM registry series was to evaluate the use of orbital atherectomy (OA) in peripheral lesions of the lower extremities, as well as optimize the technique of OA. Methods of treating calcified arteries (historically a strong predictor of treatment failure) have improved significantly over the past decade and now include minimally invasive endovascular treatments, such as OA with unique versatility in modifying calcific lesions above and below-the-knee. Patients (3135) undergoing OA by more than 350 physicians at over 200 US institutions were enrolled on an "all-comers" basis, resulting in registries that provided site-reported patient demographics, ABI, Rutherford classification, co-morbidities, lesion characteristics, plaque morphology, device usage parameters, and procedural outcomes. Treatment with OA reduced pre-procedural stenosis from an average of 88-35%. Final residual stenosis after adjunctive treatments, typically low-pressure percutaneous transluminal angioplasty (PTA), averaged 10%. Plaque removal was most effective for severely calcified lesions and least effective for soft plaque. Shorter spin times and smaller crown sizes significantly lowered procedural complications which included slow flow (4.4%), embolism (2.2%), and spasm (6.3%), emphasizing the importance of treatment regimens that focus on plaque modification over maximizing luminal gain. The OA technique optimization, which resulted in a change of device usage across the CONFIRM registry series, corresponded to a lower incidence of adverse events irrespective of calcium burden or co-morbidities. Copyright © 2013 The Authors. Wiley Periodicals, Inc.

  4. Nuclear analytical methods for trace element studies in calcified tissues

    International Nuclear Information System (INIS)

    Chaudhry, M.A.; Chaudhry, M.N.

    2001-01-01

    Full text: Various nuclear analytical methods have been developed and applied to determine the elemental composition of calcified tissues (teeth and bones). Fluorine was determined by prompt gamma activation analysis through the 19 F(p,ag) 16 O reaction. Carbon was measured by activation analysis with He-3 ions, and the technique of Proton-Induced X-ray Emission (PIXE) was applied to simultaneously determine Ca, P, and trace elements in well-documented teeth. Dental hard tissues, enamel, dentine, cement, and their junctions, as well as different parts of the same tissue, were examined separately. Furthermore, using a Proton Microprobe, we measured the surface distribution of F and other elements on and around carious lesions on the enamel. The depth profiles of F, and other elements, were also measured right up to the amelodentin junction

  5. Cost-effectiveness analysis of orbital atherectomy plus balloon angioplasty vs balloon angioplasty alone in subjects with calcified femoropopliteal lesions

    Science.gov (United States)

    Weinstock, Barry; Dattilo, Raymond; Diage, Tiffini

    2014-01-01

    Introduction As cost considerations become increasingly critical when selecting optimal endovascular treatment strategies, a cost-benefit analysis was conducted comparing the Diamondback 360°® Orbital Atherectomy System (OAS) (Cardiovascular Systems, Inc., St Paul, MN, USA) and balloon angioplasty (BA) vs BA alone for treatment of calcified femoropopliteal lesions. Patients and methods The clinical outcomes from COMPLIANCE 360°, a prospective, multicenter, randomized study comparing OAS+BA vs BA alone for treatment of calcified femoropopliteal lesions, were correlated with cost data and previously published quality of life data. Site of service, hospital charges, and associated medical resource utilization were obtained from Uniform Billing statements for index treatments and associated revascularizations out to 1 year. Hospital costs were estimated using hospital-specific, procedure-specific cost-to-charge ratios. Length of stay and procedural data were collected from participating study sites. Results Twenty-five subjects with 38 lesions and 25 subjects with 27 lesions were randomized to the OAS+BA and BA-alone groups, respectively. Mean hospital charges (US$51,755 vs US$39,922) and estimated hospital costs (US$15,100 vs US$11,016) were higher for OAS+BA compared with BA alone (not statistically significant). Stent utilization was statistically significantly higher with BA-alone treatment for all subjects (1.1 vs 0.1, P=0.001) and in the subset of subjects with one lesion (1.0 vs 0.1, Patherectomy for treatment of calcified femoropopliteal lesions, a longstanding challenge for peripheral artery disease interventionalists. PMID:24672251

  6. Natural radionuclides in soils from Sao Paulo State cerrado forest

    International Nuclear Information System (INIS)

    Miranda, Marcia V.F.E.S.; Farias, Emerson E.G. de; Cantinha, Rebeca S.; Franca, Elvis J. de

    2015-01-01

    Considering the long life history, forests should be preferentially evaluated for the monitoring of radionuclides, mainly artificial radioisotopes. However, little is known about nuclides from Uranium and Thorium series, as well as, K-40, in soils from the Sao Paulo State forests. Soils are the main reservoir of natural radionuclides for vegetation, thereby deserving attention. Taking into account the advantages of High-Resolution Gamma-ray Spectrometry (HRGS), diverse radionuclides can be quantified simultaneously. In this work natural radionuclides in soils from the Estacao Ecologica de Assis were evaluated by HRGS. Samples of 0-10 cm depth were collected under crown projection of most abundant tree species of long-term plots installed within the Estacao Ecologica de Assis, Sao Paulo State, Brazil. After drying and milling until 0.5 mm particle size, test portions of 30 g were transferred to polypropylene vials, sealed with silicone and kept under controlled conditions until 30 days to achieve secular equilibrium. A group of gamma-ray spectrometers was used to analyze about 27 samples by 80,000 seconds. Activity concentrations of Pb-214, Ac-228 and K-40 and their respective expanded analytical uncertainties at the 95% confidence level were calculated by Genie software from Canberra. Abnormal values were not detected for radionuclides in soils samples, however K-40 activity concentrations changed considerably due to the mineral cycling, in which K and, consequently K-40, is mainly stocked in vegetation in spite of soils. (author)

  7. Natural radionuclides in soils from Sao Paulo State cerrado forest

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Marcia V.F.E.S.; Farias, Emerson E.G. de; Cantinha, Rebeca S.; Franca, Elvis J. de, E-mail: mvaleria@cnen.gov.br, E-mail: emersonemiliano@yahoo.com.br, E-mail: rebecanuclear@gmail.com, E-mail: ejfranca@cnen.gov.br [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2015-07-01

    Considering the long life history, forests should be preferentially evaluated for the monitoring of radionuclides, mainly artificial radioisotopes. However, little is known about nuclides from Uranium and Thorium series, as well as, K-40, in soils from the Sao Paulo State forests. Soils are the main reservoir of natural radionuclides for vegetation, thereby deserving attention. Taking into account the advantages of High-Resolution Gamma-ray Spectrometry (HRGS), diverse radionuclides can be quantified simultaneously. In this work natural radionuclides in soils from the Estacao Ecologica de Assis were evaluated by HRGS. Samples of 0-10 cm depth were collected under crown projection of most abundant tree species of long-term plots installed within the Estacao Ecologica de Assis, Sao Paulo State, Brazil. After drying and milling until 0.5 mm particle size, test portions of 30 g were transferred to polypropylene vials, sealed with silicone and kept under controlled conditions until 30 days to achieve secular equilibrium. A group of gamma-ray spectrometers was used to analyze about 27 samples by 80,000 seconds. Activity concentrations of Pb-214, Ac-228 and K-40 and their respective expanded analytical uncertainties at the 95% confidence level were calculated by Genie software from Canberra. Abnormal values were not detected for radionuclides in soils samples, however K-40 activity concentrations changed considerably due to the mineral cycling, in which K and, consequently K-40, is mainly stocked in vegetation in spite of soils. (author)

  8. Chemical characterization of organic particulate matter from on-road traffic in Sao Paulo, Brazil

    NARCIS (Netherlands)

    Oyama, Beatriz Sayuri; Andrade, Maria de Fatima; Herckes, Pierre; Dusek, Ulrike; Rockmann, Thomas; Holzinger, Rupert

    2016-01-01

    This study reports emission of organic particulate matter by light-duty vehicles (LDVs) and heavy-duty vehicles (HDVs) in the city of Sao Paulo, Brazil, where vehicles run on three different fuel types: gasoline with 25% ethanol (called gasohol, E25), hydrated ethanol (E100), and diesel (with 5%

  9. Rb-Sr geochronology from Sao Felix to Xingu: preliminary results

    International Nuclear Information System (INIS)

    Lafon, J.M.; Pereira, E.D.; Silva Barradas, J.A. da

    1991-01-01

    Rb-Sr systematics have been applied on rocks from the Sao Felix do Xingu region, in the southeastern part of the State of Para. An age of 2716 ± 34 Ma with IR of 0.70292 ± 0.00030 (MSWD = 1.54) and an age of 2677 ± 50 Ma with IR of 0.70161 ± 0.00022 (MSWD = 5.21) had been obtained for a granitic body and a tonalitic body respectively, both of them associated to the green stones belts that occur in this area. Gneisses from the Xingu complex gave an age of 2574 ± 57 Ma with an IR of 0.70416 ± 0.00054 (MSWD = 5.06). An age of 1653 ± 14 Ma with IR of 0.70823 ± 0.02361 (MSWD = 1.71) had been obtained for the Velho Guilherme granite that crosscut the granite-green stones terrains. Such geochronological data show the existence of a magmatic event between 2.72 Ga and 2.68 Ga and permit us to conclude on the archaean age of the green stones terrains. (author)

  10. Investigation of wind characteristics and wind energy assessment in Sao Joao do Cariri (SJC) - Paraiba, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Laerte; Filho, Celso

    2010-09-15

    In this study wind characterization and wind energy assessment of the Sao Joao do Cariri (SJC) in Paraiba state situated in Brazilian northeast. The average wind speed and temperature for 25 and 50 m were found 4,74m/s, 24,46C and 5,31m/s 24,25C with wind speed predominate direction of SSE (165 degrees). Weibull shape, scale ,Weibull fit wind speed and Power wind density found 2,54, 5,4m/s, 4,76m/s and 103W/m2 for 25m wind height measurements and 2,59, 6,0m/s, 5,36m/s and 145W/m2 for 50m wind height measurements.

  11. Food group contribution of essential elements of the Sao Paulo State market basket

    International Nuclear Information System (INIS)

    Avegliano, Roseane P.; Maihara, Vera A.; Silva, Fabio F. da

    2009-01-01

    To establish a Market Basket of Sao Paulo state seventy-one foods, with a mean consumption of more than 2 g day -1 per person, were grouped into 30 food categories. The food groups were: cereals, leguminous, leafy vegetables, fruity vegetables, tuberous vegetables, tropical fruits, other fruits, flours, pastas, breads, biscuits, prime grade beef, standard grade beef, pork meats, other meats, poultry, milk/cream, other dairy products, sugars, sweets, salts, sauces, oils, fats, alcoholic beverages, non-alcoholic beverages, coffee, ready-made dishes, saltwater and freshwater fishes. Information about individual food consumption was obtained from a recent national household food budget survey 'POF 2002-2003' conducted by the Brazilian Institute for Geography and Statistics from July 2002 to June 2003. Sampling and kitchen preparation of foods were carried out in restaurants of the University of Sao Paulo. Each food item was individually prepared table-ready. Foods of the same group were mixed, homogenized, pulverized and analyzed for the determination of Ca, Cr, Fe, K, Na and Zn concentrations by Instrumental Neutron Activation Analysis. Average daily intake of each element was calculated by multiplying the element concentration in the food by the corresponding weight of the ready-to-consume food group. The contribution of each food group to the total daily intake of elements by the ready-to-consume food groups of the Market Basket was evaluated. The food groups representing the highest contributions were salts: 79% Na; breads: 37% Fe and 46% Cr; cereals: 19% Zn and milk/cream: 58% Ca and 24% K. (author)

  12. Cost-effectiveness analysis of orbital atherectomy plus balloon angioplasty vs balloon angioplasty alone in subjects with calcified femoropopliteal lesions

    Directory of Open Access Journals (Sweden)

    Weinstock B

    2014-03-01

    Full Text Available Barry Weinstock,1 Raymond Dattilo,2 Tiffini Diage3 1Orlando Health Heart Institute, Mid-Florida Cardiology Specialists, Orlando, FL, USA; 2Department of Cardiology, St Francis Health Center, Topeka, KS, USA; 3North American Science Association (NAMSA, Sunnyvale, CA, USA Introduction: As cost considerations become increasingly critical when selecting optimal endovascular treatment strategies, a cost-benefit analysis was conducted comparing the Diamondback 360°® Orbital Atherectomy System (OAS (Cardiovascular Systems, Inc., St Paul, MN, USA and balloon angioplasty (BA vs BA alone for treatment of calcified femoropopliteal lesions. Patients and methods: The clinical outcomes from COMPLIANCE 360°, a prospective, multicenter, randomized study comparing OAS+BA vs BA alone for treatment of calcified femoropopliteal lesions, were correlated with cost data and previously published quality of life data. Site of service, hospital charges, and associated medical resource utilization were obtained from Uniform Billing statements for index treatments and associated revascularizations out to 1 year. Hospital costs were estimated using hospital-specific, procedure-specific cost-to-charge ratios. Length of stay and procedural data were collected from participating study sites. Results: Twenty-five subjects with 38 lesions and 25 subjects with 27 lesions were randomized to the OAS+BA and BA-alone groups, respectively. Mean hospital charges (US$51,755 vs US$39,922 and estimated hospital costs (US$15,100 vs US$11,016 were higher for OAS+BA compared with BA alone (not statistically significant. Stent utilization was statistically significantly higher with BA-alone treatment for all subjects (1.1 vs 0.1, P=0.001 and in the subset of subjects with one lesion (1.0 vs 0.1, P<0.00001. There was a significant difference in cost for single-lesion versus multiple-lesion treatment. Using costs and quality-adjusted life years (QALYs for the single-lesion cohort, the 1-year

  13. Ionizing radiation population doses at Sao Paulo city, Brazil: open-pit gamma dose measurement

    International Nuclear Information System (INIS)

    Oliveira, Raimundo Enoch Rodrigues

    2001-01-01

    The effects of ionizing radiation to the human beings are well known for high and intermediate doses. As far as low level) radiation doses are concerned, there is no consensus. In order to get a better understanding of such effects it is necessary to assess the low doses with better accuracy. In this work, it was made an estimate of the annual ambient dose equivalent (H * (10)) to which the people are exposed in the city of Sao Paulo. Until now there are no data about it available in the literature. For the purpose of this evaluation, a map with various routes covering the largest and more representative area of the city was designed. The choice of points for data collection was made taking into account mainly the occupancy of the region. A portable gamma spectrometry system was used. It furnishes the rate of H * (10) and the measured gamma spectrum (in the range from 50 to 1670 keV) in the place of interest. The measurements were performed in a short time interval, since the gamma radiation arrives from a great extent of soil. Each measurement was done 1 m above the soil during 300 s. The rates of H * (10) varied from 33.1 to 152.3 nSv.h -1 , net values, obtained after subtraction of the cosmic rays contribution. The standard deviation was 22 n Sv.h -1 for an average for the city of Sao Paulo of 96.1(24) nSv.h -1 . In addition, average values of H * (10) rates for the city Health Divisions were calculated. Those values are not statistically equivalent and the whole set of data could not be treated as one, as the statistical Student test indicated a non homogeneity of the group of data. Hence it is necessary the accomplishment of a more detailed survey in order to verify the origin of the discrepancy. The mean value of H * (10) rate obtained for the city of Sao Paulo as converted to effective dose. in order to be compared with other places results It could be noticed that the annual average of effective dose for the city of Sao Paulo, 0.522(13) mSv, is superior to

  14. The study of ground water movements about Guarani Aquifer system through Parana, Sao Paulo isotopes and Uruguay

    International Nuclear Information System (INIS)

    Da Rosa Filho, E.; Lima Bittendourt, A.; Chemas Hindt, E.; Duarte, U.; Montano, J.

    2004-01-01

    This study relates tectonic features to hydrochemistry properties in order to show an example where the Guarany Aquifer behaves like a closed compartiment. This situation can be used as a model to other localities with similar geological structures where the aquifer is not recharged by modern waters, therefore, groundwater withdrawal from deep wells depletes the aquifer turning its exploitation not recommended. This study area was located inside the limits of the Ponta Grossa Arch, especially by the the Rio Alonzo and Sao Jeronimo-Curiuva lineaments. Among the waters that had been already classified in Sao Paulo and Parana States (Brazil), predominate the calcium-bicarbonate and sodiumbicarbonate types and the characteristic of the water in this particular case is sodiumsulfate- chloride type with TDS above 1000 mg/L

  15. Global gene expression analysis of canine osteosarcoma stem cells reveals a novel role for COX-2 in tumour initiation.

    Science.gov (United States)

    Pang, Lisa Y; Gatenby, Emma L; Kamida, Ayako; Whitelaw, Bruce A; Hupp, Ted R; Argyle, David J

    2014-01-01

    Osteosarcoma is the most common primary bone tumour of both children and dogs. It is an aggressive tumour in both species with a rapid clinical course leading ultimately to metastasis. In dogs and children distant metastasis occurs in >80% of individuals treated by surgery alone. Both canine and human osteosarcoma has been shown to contain a sub-population of cancer stem cells (CSCs), which may drive tumour growth, recurrence and metastasis, suggesting that naturally occurring canine osteosarcoma could act as a preclinical model for the human disease. Here we report the successful isolation of CSCs from primary canine osteosarcoma, as well as established cell lines. We show that these cells can form tumourspheres, and demonstrate relative resistance to chemotherapy. We demonstrate similar results for the human osteosarcma cell lines, U2OS and SAOS2. Utilizing the Affymetrix canine microarray, we are able to definitively show that there are significant differences in global gene expression profiles of isolated osteosarcoma stem cells and the daughter adherent cells. We identified 13,221 significant differences (p = 0.05), and significantly, COX-2 was expressed 141-fold more in CSC spheres than daughter adherent cells. To study the role of COX-2 expression in CSCs we utilized the COX-2 inhibitors meloxicam and mavacoxib. We found that COX-2 inhibition had no effect on CSC growth, or resistance to chemotherapy. However inhibition of COX-2 in daughter cells prevented sphere formation, indicating a potential significant role for COX-2 in tumour initiation.

  16. Global gene expression analysis of canine osteosarcoma stem cells reveals a novel role for COX-2 in tumour initiation.

    Directory of Open Access Journals (Sweden)

    Lisa Y Pang

    Full Text Available Osteosarcoma is the most common primary bone tumour of both children and dogs. It is an aggressive tumour in both species with a rapid clinical course leading ultimately to metastasis. In dogs and children distant metastasis occurs in >80% of individuals treated by surgery alone. Both canine and human osteosarcoma has been shown to contain a sub-population of cancer stem cells (CSCs, which may drive tumour growth, recurrence and metastasis, suggesting that naturally occurring canine osteosarcoma could act as a preclinical model for the human disease. Here we report the successful isolation of CSCs from primary canine osteosarcoma, as well as established cell lines. We show that these cells can form tumourspheres, and demonstrate relative resistance to chemotherapy. We demonstrate similar results for the human osteosarcma cell lines, U2OS and SAOS2. Utilizing the Affymetrix canine microarray, we are able to definitively show that there are significant differences in global gene expression profiles of isolated osteosarcoma stem cells and the daughter adherent cells. We identified 13,221 significant differences (p = 0.05, and significantly, COX-2 was expressed 141-fold more in CSC spheres than daughter adherent cells. To study the role of COX-2 expression in CSCs we utilized the COX-2 inhibitors meloxicam and mavacoxib. We found that COX-2 inhibition had no effect on CSC growth, or resistance to chemotherapy. However inhibition of COX-2 in daughter cells prevented sphere formation, indicating a potential significant role for COX-2 in tumour initiation.

  17. Determination of essential and toxic elements in sea bass (Centropomus sp.) and hake (Cynoscion leiarchus) consumed in the city of Sao Paulo by neutron activation analysis

    International Nuclear Information System (INIS)

    Fabiano, Karen C.; Moreira, Edson G.; Vasconcellos, Marina B.A.

    2013-01-01

    The goal was to use the Instrumental Neutron Activation Analysis (INAA) to evaluate the levels of essential and toxic elements in samples of hake (Cynoscion leiarchus) and sea bass (Centropomus sp.) that are most consumed fish in the town of some Sao Paulo. Ten specimens of hake and sea bass were acquired in Companhia de Entrepostos e Armazens Gerais de Sao Paulo (CEAGESP), main supply center for the population of the metropolitan area of Sao Paulo. After gutted and cleaned, the edible tissue was freeze-dried, ground and sieved. The elements were determined through the use of hyperpure germanium spectrometer after irradiation in the nuclear research reactor IEAR-1

  18. Calcified neurocysticercosis lesions and hippocampal sclerosis: potential dual pathology?

    Science.gov (United States)

    Rathore, Chaturbhuj; Thomas, Bejoy; Kesavadas, Chandrasekharan; Radhakrishnan, Kurupath

    2012-04-01

    In areas where cysticercosis is endemic, calcified neurocysticercosis lesion(s) (CNL) and hippocampal sclerosis (HS) commonly coexist in patients with localization-related epilepsies. To understand the pathogenesis of HS associated with CNL, we compared the characteristics of three groups of patients with antiepileptic drug-resistant epilepsies: CNL with HS, CNL without HS (CNL alone), and HS without CNL (HS alone). In comparison to patients with CNL alone, those with CNL with HS had CNL more frequently located in the ipsilateral temporal lobe. Those with CNL with HS had a lower incidence of febrile seizures, older age at initial precipitating injury and at onset of habitual complex partial seizures, and more frequent clustering of seizures and extratemporal/bitemporal interictal epileptiform discharges as compared to patients with HS alone. Our study illustrates that HS associated with CNL might have a different pathophysiologic basis as compared to classical HS. Wiley Periodicals, Inc. © 2012 International League Against Epilepsy.

  19. Annual report 2007 - CPFL Energy - Light and Power Company from Sao Paulo, SP, Brazil

    International Nuclear Information System (INIS)

    2008-01-01

    This document presents the balance of the economic, social, environmental performance of the Sao Paulo Light and Power Company (CPFL), during the year of 2007 as follows: highlights of 2007; profile of the CPFL Energy group; corporative performance; strategy of the company; performance in 2007 (the best year of the company); social responsibility

  20. The Sao Jose do Rio Pardo mangeritic-granitic suite, south eastern Brazil

    International Nuclear Information System (INIS)

    Campos Neto, M.C.; Figueiredo, M.C.H.; Janasi, V.A.; Basei, M.A.S.; Fryer, B.J.

    1988-01-01

    In the Sao Jose do Rio Pardo region, Sao Paulo and Minas Gerais States, occur some intrusive, folded tabular bodies of mangerites associated with hornblende granitoids. The country rocks correspond to a complex association of gneisses and migmatites, locally with granulite facies assemblages. Both the magnerites and hornblende granitoids present a tectonic foliation with mineral flattening and stretching. Petrographically the mangeritic rocks are mainly dark green quartz mangerites with mesoperthite, plagioclase, quartz, hypersthene, clinopyroxene and variable amounts of hornblende, with zircon as conspicuous acessory. The pink hornblende granitoids are mainly granite s.s. exhibiting higher quartz and amphibole contents and lacking pyroxenes. Hololeucocratic alkali feldspar granites are locally associated to the hornblende granites. The textures of the mangerites and granites almost always show an important metamorphic overprinting, with relictic mesoperthite and pyroxene crystal into a granoblastic matrix. The magneritic-granitic suite is characterized by relatively high Fe/(Fe + Mg), K and HFS elements and low Ca contents, being comparable to typical anorogenic magneritic-granitic suites from Svcandinavia and North America. The Rb/Sr data indicate a Late Proterozoic metamorphic isotopic rehomogenization (930 Ma, Ro = 0.706). Geological evidence suggest that the intrusive age could be Middle Proterozoic, wich is reinforced by another Rb-Sr value of about 1300 Ma. (author) [pt

  1. Backscattered electron imaging: The role in calcified tissue and implant analysis

    International Nuclear Information System (INIS)

    Bloebaum, R.D.; Bachus, K.N.; Boyce, T.M.

    1990-01-01

    The working distance and tilt studies helped to clarify the influences of specimen variability when the BSE mode is used in calcified tissue research. This work has shown that the BSEPs of cortical bone may be accurately maintained within 2 percent error over a 10 degree range of tilt, or 300 microns working distance variation. If future bone and implant investigators wish to conduct accurate, quantitative mineral microanalysis in bone, then standard grinding and polishing techniques should be adequate if calibration procedures are developed. The BSEP characteristics of the pure metals make them suitable to be used for calibrating the BSE signal. BSE analysis, with correlated biomechanical studies, will lead us to a better understanding of the relationships between structure, function, and mineral content in bone. On-line BSEP analysis techniques will expand our understanding of the mineralization events in bone which are associated with aging, weightlessness, pharmaceutical therapies, and the presence of biomaterials. The future of the BSE imaging technology and the contributions to be made in understanding the histometry, biomechanics and mineral content of bone as well as bone's response to implant materials has just begun to unfold. 74 references

  2. Behavior of bone cells in contact with magnesium implant material.

    Science.gov (United States)

    Burmester, Anna; Willumeit-Römer, Regine; Feyerabend, Frank

    2017-01-01

    Magnesium-based implants exhibit several advantages, such as biodegradability and possible osteoinductive properties. Whether the degradation may induce cell type-specific changes in metabolism still remains unclear. To examine the osteoinductivity mechanisms, the reaction of bone-derived cells (MG63, U2OS, SaoS2, and primary human osteoblasts (OB)) to magnesium (Mg) was determined. Mg-based extracts were used to mimic more realistic Mg degradation conditions. Moreover, the influence of cells having direct contact with the degrading Mg metal was investigated. In exposure to extracts and in direct contact, the cells decreased pH and osmolality due to metabolic activity. Proliferating cells showed no significant reaction to extracts, whereas differentiating cells were negatively influenced. In contrast to extract exposure, where cell size increased, in direct contact to magnesium, cell size was stable or even decreased. The amount of focal adhesions decreased over time on all materials. Genes involved in bone formation were significantly upregulated, especially for primary human osteoblasts. Some osteoinductive indicators were observed for OB: (i) an increased cell count after extract addition indicated a higher proliferation potential; (ii) increased cell sizes after extract supplementation in combination with augmented adhesion behavior of these cells suggest an early switch to differentiation; and (iii) bone-inducing gene expression patterns were determined for all analyzed conditions. The results from the cell lines were inhomogeneous and showed no specific stimulus of Mg. The comparison of the different cell types showed that primary cells of the investigated tissue should be used as an in vitro model if Mg is analyzed. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 165-179, 2017. © 2015 Wiley Periodicals, Inc.

  3. The electric energy consumption in the city of Sao Paulo - Brazil: the residential sector in the eighties; O consumo de energia eletrica na cidade de Sao Paulo: o setor residencial nos anos oitenta

    Energy Technology Data Exchange (ETDEWEB)

    Borelli, A.B.; Sellito, Y.M.; Moreira, J.G.S. [Sao Paulo Univ., SP (Brazil). Inst. de Eletrotecnica e Energia

    1994-07-01

    This work analyses the evolution of the energy consumption in the residential sector of the city of Sao Paulo in the years of 19980, 1985 and 1990, and relates electric power cost participation in the family monthly stratified budget. Also, the price x consumption elasticity verified in the mentioned period is analysed.

  4. Phurcalite and others secondary uranium minerals from Perus, Sao Paulo, Brazil; Furcalita e outros minerais uraniferos secundarios de Perus, Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Atencio, D

    1992-12-31

    Phurcalite has been found filling fractures in the tourmaline-bearing granitic pegmatite of Perus, in the north-west part of Sao Paulo city, Brazil. It forms aggregates of radiating euhedral crystals up to 5 mm in length. The crystals are bright yellow, transparent and display vitreous to adamantine lustre. Its streak is pale yellow. Phurcalite is brittle, with a conchoidal fracture, and non-fluorescent. The crystal structure of phurcalite has been solves by single-crystal x-ray diffraction methods and refined to R = 3.8% using 2065 observed [I > 3{sigma}(I)] reflections. The structure consists of [(U O{sub 2}){sub 3} O{sub 2} (P O{sub 4}){sub 2} {sup 4n-}]{sub n} layers, parallel to (010), connected by Ca{sup 2+} ions and H{sub 2} O. The coordination polyhedra are: for U(1) hexagonal bi pyramid; for U(2) and U(3) pentagonal bi pyramids; for Ca(4) and Ca(5) capped trigonal prism and triangulated dodecahedron, respectively; and for P(6) and P(7) tetrahedra. As a consequence of this work, the molecular formula of phurcalite previously reported as Ca{sub 2} (U O{sub 2}){sub 3} (P O{sub 4}){sub 2} (OH){sub 4}.4 H{sub 2} O must be changed to Ca{sub 2} (U O{sub 2}){sub 3} O{sub 2} (P O{sub 4}){sub 2}.7 H{sub 2} O. Other secondary uranium minerals associated with Perus phurcalite are autunite, torbernite, meta-autunite, meta-torbernite, chernikovite, meta-uranocircite I, phosphuranylite, uranophane-alpha, uranophane-beta, haiweeite, barian week site and perhaps also bassetite, meta-tyuyamunite and meta-haiweeite. Opal, tridymite, cristobalite, secondary quartz, saponite and rhodochrosite occur associated to the uranium minerals. (author).

  5. Legal protection of the Guarani aquifer in Ribeirao Preto (Sao Paulo State, Brasil); Tutela juridica do Aquifero Guarani em Ribeirao Preto (Estado de Sao Paulo, Brasil)

    Energy Technology Data Exchange (ETDEWEB)

    Pedroso Goulart, M.; Cavalheiro Navajas Sampaio Campos, H.; Nepomuceno, O.

    2012-11-01

    The Guarani aquifer is one of the largest reservoirs of groundwater in the world and represents a strategic reserve for the four countries of South America where it occurs: Argentina, Brazil, Paraguay and Uruguay. The municipality of Ribeirao Preto, located in the north-east region of Sao Paulo, with a population of over 600,000 inhabitants, is supplied entirely by water from this aquifer. Hydrogeological studies reveal the existence of a large cone of drawdown in the centre of the city due to the intensive and indiscriminate use of water pumped out by wells for decades. In rural areas, where many of the rocky outcrops of the aquifer occur and which affords direct recharge by rain water, the risk of contamination by pesticides is of some concern. Over the years the Public Ministry of Sao Paulo in Ribeirao Preto has provided guidelines for actions and initiatives in environmental protection, especially with regard to the Guarani aquifer. These actions and initiatives are based mainly on the principle of the supremacy of public interest over private interest and the principle of caution. On the basis of these premises the prosecutor works with the government and the private sector to monitor and readmitted environmental liabilities that threaten the aquifer (landfills, diffuse contamination etc.) and to maintain due discipline within the activities that take place within the recharge zone (agriculture, industry and urbanization) including those of regulation and territorial planning. (Author)

  6. Clear cell chondrosarcoma

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, R.; David, R.; Cierney, G. III

    1985-01-01

    The clinical, radiologic, and histopathologic features of three cases of clear cell chondrosarcoma are described. On radiographs, this rather benign-appearing tumor resembles a chondroblastoma when it occurs at the end of a long bone, and may occasionally show a calcified matrix. However, it has distinctive tumor cells with a centrally placed vesicular nucleus surrounded by clear cytoplasm. The lesion has a low-grade malignancy and is amenable to en bloc surgical resection, which results in a much better prognosis than that of conventional chondrosarcoma.

  7. Physicochemical characterization of ceramics from Sao Paulo II archaeological site

    International Nuclear Information System (INIS)

    Ribeiro, Rogerio Baria

    2013-01-01

    Archaeometry is a consolidated field with a wide application of nuclear analytical techniques for the characterization, protection, and restoration of archaeological pieces. This project aimed at studying the elementary chemical composition of 70 ceramic fragments samples from Sao Paulo II archaeological site, located along the Solimoes River channel, next to Coari city, in Brazilian Amazon. The characterization of samples was performed by neutron activation analysis (NAA). By the determination of 24 elements in the ceramic fragments ( Ce, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Na, Nd, Sb, Sm. Rb, Se, Ta, Tb, Th, U, Yb and Zn), it was possible to define groups of samples regarding the similarity/dissimilarity in elementary chemical composition. For such a task, the multivariate statistical methods employed were cluster analysis (C A), principal component analysis (PCA) and discriminant analysis (DA). Afterwards, seven ceramic fragments were selected based on the groups previously established, for the characterization of the site temporal horizon. Those ceramic fragments were analyzed by thermoluminescence (TL) and EPR for dating purposes. The firing temperatures were determined by electron paramagnetic resonance (EPR) technique, in order to infer about some aspects of the ceramic manufacture employed by the ancient peoples that lived in Sao Paulo 11. By the results obtained in this study, it was possible to identify the quantity of clay sources employed by the ceramists and the age of the ceramic pieces. Therefore, the results of this research may contribute to the study on the occupation dynamics in the pre-colonial Brazilian Amazon. (author)

  8. Environmental license process complexity in natural protected areas: case of OSBAT pipeline operated by TRANSPETRO in the state of Sao Paulo; Complexidade do licenciamento ambiental em areas naturais protegidas: caso do duto OSBAT operado pela TRANSPETRO no estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Anibal Jose Constantino; Raul, Vanderlei Lourenco; Martini, Andrea Dietrich; Campos, Cleber Lucio dos Santos; Ozorio, Tarcisio Faria; Melo Neto, Joao Evangelista de; Lima, Silvia Ferreira de [PETROBRAS Transporte S. A. (TRANSPETRO), Rio de Janeiro, RJ (Brazil)

    2008-07-01

    It presents the environmental license process enforced to obtain the Implementation License (LI) of the OSBAT pipeway part substitution, in the Sao Sebastiao city, State of Sao Paulo, with the analyses centered on the several public power instances and on the many special legal protection aspects around the natural area scenarios of the project interference. The license process had the duration of seventeen months, since the LI protocol and its emission. The process had the involvement of several organisms, the main part from the Sistema Nacional de Meio Ambiente - SISNAMA, and represented an interesting experience that can contribute to the agility of other license process of infrastructure works on protected natural areas, which has the incidence of an extensive legislation and activities restriction. (author)

  9. Doxycyclin induces p53 expression in SaOs (osteosarcoma) cell line ...

    African Journals Online (AJOL)

    The p53 tumour suppressor gene plays an important role in preventing cancer development. This study determined if p53 can be induced in osteosarcoma cell line upon treatment ... represent an important component of the p53 tumor suppressor pathway. Keywords: Tumor suppressor, oncogene, mdm2, cyclinE, apoptosis ...

  10. Conception of the Instrument Calibration Laboratory of Ionizing Radiation Measurement (LACIMRI) of CTMSP - Sao Paulo, SP; Concepcao do Laboratorio de Calibracao de Instrumentos de Medicao de Radiacao Ionizante (LACIMRI) do CTMSP, Sao Paulo, SP

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Raimundo Dias da; Kibrit, Eduardo, E-mail: raimundo@ctmsp.mar.mil.b, E-mail: kibrit@ctmsp.mar.mil.b [Centro Tecnologico da Marinha em Sao Paulo (CTMSP), Sao Paulo, SP (Brazil)

    2009-07-01

    The present work describes the phases of implantation of calibration laboratory of ionizing radiation measurement instruments at the CTMSP, Sao Paulo, in a priory approved by CNEN, Brazil. That laboratory will allow and enhance the present metrological capacity for the attendance to the growing demand for calibration services of the instruments

  11. Effect of ethanol on human osteosarcoma cell proliferatation, differentiation and mineralization

    International Nuclear Information System (INIS)

    Vignesh, R.C.; Sitta Djody, S.; Jayasudha, E.; Gopalakrishnan, V.; Ilangovan, R.; Balaganesh, M.; Veni, S.; Sridhar, M.; Srinivasan, N.

    2006-01-01

    The habitual consumption of even moderate quantities of alcoholic beverages is clearly associated with reduced bone mass, increased prevalence of skeletal fracture and also it is the major risk factor for the development of secondary osteoporosis. The present in vitro study was designed to determine the dose response effects of ethanol on osteoblast-like human osteosarcoma cells (SaOS-2) proliferation, differentiation, mineralization and cyto-toxicity. SaOS-2 cells were plated in 48 and 6 well culture plates and exposed to different concentrations of ethanol (1, 10, 100, 200 and 300 mM) for 24, 48 and 72 h. At the end of incubation, proliferation of cells was studied using crystal violet Bioassay. The cell lysate was utilized to determine ALP activity and conditioned media were used to measure LDH activity. Histochemical localization of ALP and mineralized nodules were studied from cells treated with ethanol (10 and 100 mM) for 21 days. At higher doses, there was a significant reduction in cell number, whereas at lower doses there were variable effects. In 24 h treatment, the higher doses showed a significant increase in ALP activity, whereas 48 and 72 h treatments showed an opposite trend. Ethanol treatment caused a dose- and time-dependent increase in LDH activity. Ethanol treatment altered the quality of mineralization at 10 mM dose whereas completely inhibited mineralization at 100 mM dose, despite the presence of serum. In conclusion, the toxic effect of ethanol is reflected on cell proliferation, differentiation and mineralization even at low doses and at extended treatment duration

  12. Archaeometric studies of ceramics from the Sao Paulo II archaeological site

    International Nuclear Information System (INIS)

    Rogerio Baria; Munita, C.S.; Cano, N.F.; Tatumi, S.H.; Silva-Carrera, B.N.; Shigueo Watanabe; Neves, E.G.

    2015-01-01

    This paper aims at studying the elementary chemical composition of 70 ceramic fragments from the Sao Paulo II archaeological site by instrumental neutron activation analysis. The concentration data was studied next using multivariate statistical methods, such as cluster analysis, principal component analysis and discriminant analysis. The results showed three different chemical groups of samples regarding the similarity/dissimilarity between the samples. Ceramics from each group have been selected and dated using thermoluminescence. The firing temperature of the ceramics was determined by electron paramagnetic resonance. (author)

  13. Spatial distribution of benthic macroorganisms on reef flats at Porto de Galinhas Beach (northeastern Brazil, with special focus on corals and calcified hydroids

    Directory of Open Access Journals (Sweden)

    Juliana Imenis Barradas

    2010-06-01

    Full Text Available Despite their ecological and social-economic importance, coral reefs are under constant threat and thus require proper management practices. Data on the spatial structure of these ecosystems are essential for good quality conservation projects in such areas. This study aimed to quantitatively analyze the spatial distribution of benthic macroorganisms from the reef environment of Porto de Galinhas Beach, with special focus on its corals and calcified hydroids. Reef flats of the area were surveyed by scuba diving, using 10m line transects. A high cover of macroalgae was verified, averaging 53% of the observations. Zoanthids were the second most representative group (11%. A total of 173 colonies of corals and calcified hydroids were observed and 40% of these colonies were partially or totally bleached.

  14. The SAO and Kelvin waves in the EuroGRIPS GCMS and the UK Met. Office analyses

    Directory of Open Access Journals (Sweden)

    M. Amodei

    Full Text Available We compare the tropical oscillations and planetary scale Kelvin waves in four troposphere-stratosphere climate models and the assimilated dataset produced by the United Kingdom Meteorological Office (UKMO. The comparison has been made in the GRIPS framework "GCM-Reality Intercomparison Project for SPARC", where SPARC is Stratospheric Processes and their Role in Climate, a project of the World Climate Research Program. The four models evaluated are European members of GRIPS: the UKMO Unified Model (UM, the model of the Free University in Berlin (FUB–GCM, the ARPEGE-climat model of the French National Centre for Meteorological Research (CNRM, and the Extended UGAMP GCM (EUGCM of the Centre for Global Atmospheric Modelling (CGAM. The integrations were performed with different, but annually periodic external conditions (e.g., sea-surface temperature, sea ice, and incoming solar radiation. The structure of the tropical winds and the strengths of the Kelvin waves are examined. In the analyses where the SAO (Semi-Annual Oscillation and the QBO (Quasi-Biennal Oscillation are reasonably well captured, the amplitude of these analysed Kelvin waves is close to that observed in independent data from UARS (Upper Atmosphere Research Satellite. In agreement with observations, the Kelvin waves generated in the models propagate into the middle atmosphere as wave packets, consistent with a convective forcing origin. In three of the models, slow Kelvin waves propagate too high and their amplitudes are overestimated in the upper stratosphere and in the mesosphere, the exception is the UM which has weaker waves. None of the modelled waves are sufficient to force realistic eastward phases of the QBO or SAO. Although the SAO is represented by all models, only two of them are able to generate westerlies between 10 hPa and 50 hPa. The importance of the role played in the SAO by unresolved gravity waves is emphasized. Although it exhibits some unrealistic features, the

  15. The SAO and Kelvin waves in the EuroGRIPS GCMS and the UK Met. Office analyses

    Directory of Open Access Journals (Sweden)

    M. Amodei

    2001-01-01

    Full Text Available We compare the tropical oscillations and planetary scale Kelvin waves in four troposphere-stratosphere climate models and the assimilated dataset produced by the United Kingdom Meteorological Office (UKMO. The comparison has been made in the GRIPS framework "GCM-Reality Intercomparison Project for SPARC", where SPARC is Stratospheric Processes and their Role in Climate, a project of the World Climate Research Program. The four models evaluated are European members of GRIPS: the UKMO Unified Model (UM, the model of the Free University in Berlin (FUB–GCM, the ARPEGE-climat model of the French National Centre for Meteorological Research (CNRM, and the Extended UGAMP GCM (EUGCM of the Centre for Global Atmospheric Modelling (CGAM. The integrations were performed with different, but annually periodic external conditions (e.g., sea-surface temperature, sea ice, and incoming solar radiation. The structure of the tropical winds and the strengths of the Kelvin waves are examined. In the analyses where the SAO (Semi-Annual Oscillation and the QBO (Quasi-Biennal Oscillation are reasonably well captured, the amplitude of these analysed Kelvin waves is close to that observed in independent data from UARS (Upper Atmosphere Research Satellite. In agreement with observations, the Kelvin waves generated in the models propagate into the middle atmosphere as wave packets, consistent with a convective forcing origin. In three of the models, slow Kelvin waves propagate too high and their amplitudes are overestimated in the upper stratosphere and in the mesosphere, the exception is the UM which has weaker waves. None of the modelled waves are sufficient to force realistic eastward phases of the QBO or SAO. Although the SAO is represented by all models, only two of them are able to generate westerlies between 10 hPa and 50 hPa. The importance of the role played in the SAO by unresolved gravity waves is emphasized. Although it exhibits some unrealistic features, the

  16. Control and instrumentation system of the Zero Power Reactor at IEA, Sao Paulo (Brazil)

    International Nuclear Information System (INIS)

    Peluso, M.A.V.; Matsuda, K.; Hukai, R.

    1974-01-01

    The control and instrumentation system of the Zero Power Reactor at the IEA (Institute of Atomic Energy - Sao Paulo, Brazil) is described. Technical specifications of the main items of equipment are presented in a general way. Information is also given on the connection between the system described and the electrical supply system of the IEA reactor physics laboratory [pt

  17. Tendinopatia calcárea: uma afecção local ou sistêmica? Calcifying tendinopathy: a local or a systemic condition?

    Directory of Open Access Journals (Sweden)

    Benno Ejnisman

    2012-01-01

    Full Text Available OBJETIVO: Avaliar a relação existente entre os casos de tendinopatia calcárea de ombro e doenças metabólicas sintomáticas como litíase renal, biliar e gota. MÉTODO: A tendinopatia calcárea de ombro foi diagnosticada em 63 pacientes no período compreendido entre maio de 2007 e setembro de 2011. Todos os pacientes foram tratados pelo mesmo médico ortopedista e entrevistados para coleta dos seguintes dados: idade ao diagnóstico, sexo, lado acometido, lado dominante, índice de massa corpórea (IMC, tabagismo e história pregressa de litíase renal, biliar ou gota. Para análise estatística, um grupo controle de 63 pacientes com características demográficas semelhantes foi utilizado. RESULTADOS: Dos 63 pacientes que apresentavam tendinopatia calcárea do ombro, 35 (56% eram do sexo masculino. O lado direito foi afetado em 38 (60% pacientes, a média de idade foi de 48,2 anos. Trinta e um (49% pacientes apresentaram história prévia de alguma das doenças metabólicas questionadas, sendo que 20 pacientes (32% relataram litíase renal, seis (9,5% litíase biliar, quatro (6,3% gota e um (2% apresentava diagnóstico concomitante de litíase renal e gota. No grupo controle, observamos que 11 (17% pacientes apresentaram história prévia de alguma das doenças metabólicas, sendo que seis pacientes (9,5% relataram litíase renal, quatro (6,3% litíase biliar e um (1,6%, gota. CONCLUSÕES: A elevada frequência de litíase renal em pacientes diagnosticados com tendinopatia calcárea do ombro no presente estudo sugere que existam mecanismos em comum na fisiopatologia desses distúrbios. O melhor entendimento destas doenças pode possibilitar a melhoria dos seus diagnósticos e tratamentos.OBJECTIVE: To evaluate the relationship between cases of calcifying tendinopathy of the shoulder and symptomatic metabolic diseases such as kidney stones, gallstones and gout. METHODS: Calcifying tendinopathy of the shoulder was diagnosed in 63 patients

  18. Ocean acidification and calcifying reef organisms: a mesocosm investigation

    Science.gov (United States)

    Jokiel, P. L.; Rodgers, K. S.; Kuffner, I. B.; Andersson, A. J.; Cox, E. F.; MacKenzie, F. T.

    2008-09-01

    A long-term (10 months) controlled experiment was conducted to test the impact of increased partial pressure of carbon dioxide ( pCO2) on common calcifying coral reef organisms. The experiment was conducted in replicate continuous flow coral reef mesocosms flushed with unfiltered sea water from Kaneohe Bay, Oahu, Hawaii. Mesocosms were located in full sunlight and experienced diurnal and seasonal fluctuations in temperature and sea water chemistry characteristic of the adjacent reef flat. Treatment mesocosms were manipulated to simulate an increase in pCO2 to levels expected in this century [midday pCO2 levels exceeding control mesocosms by 365 ± 130 μatm (mean ± sd)]. Acidification had a profound impact on the development and growth of crustose coralline algae (CCA) populations. During the experiment, CCA developed 25% cover in the control mesocosms and only 4% in the acidified mesocosms, representing an 86% relative reduction. Free-living associations of CCA known as rhodoliths living in the control mesocosms grew at a rate of 0.6 g buoyant weight year-1 while those in the acidified experimental treatment decreased in weight at a rate of 0.9 g buoyant weight year-1, representing a 250% difference. CCA play an important role in the growth and stabilization of carbonate reefs, so future changes of this magnitude could greatly impact coral reefs throughout the world. Coral calcification decreased between 15% and 20% under acidified conditions. Linear extension decreased by 14% under acidified conditions in one experiment. Larvae of the coral Pocillopora damicornis were able to recruit under the acidified conditions. In addition, there was no significant difference in production of gametes by the coral Montipora capitata after 6 months of exposure to the treatments.

  19. Ocean acidification and calcifying reef organisms: A mesocosm investigation

    Science.gov (United States)

    Jokiel, P.L.; Rodgers, K.S.; Kuffner, I.B.; Andersson, A.J.; Cox, E.F.; MacKenzie, F.T.

    2008-01-01

    A long-term (10 months) controlled experiment was conducted to test the impact of increased partial pressure of carbon dioxide (pCO2) on common calcifying coral reef organisms. The experiment was conducted in replicate continuous flow coral reef mesocosms flushed with unfiltered sea water from Kaneohe Bay, Oahu, Hawaii. Mesocosms were located in full sunlight and experienced diurnal and seasonal fluctuations in temperature and sea water chemistry characteristic of the adjacent reef flat. Treatment mesocosms were manipulated to simulate an increase in pCO2 to levels expected in this century [midday pCO2 levels exceeding control mesocosms by 365 ?? 130 ??atm (mean ?? sd)]. Acidification had a profound impact on the development and growth of crustose coralline algae (CCA) populations. During the experiment, CCA developed 25% cover in the control mesocosms and only 4% in the acidified mesocosms, representing an 86% relative reduction. Free-living associations of CCA known as rhodoliths living in the control mesocosms grew at a rate of 0.6 g buoyant weight year-1 while those in the acidified experimental treatment decreased in weight at a rate of 0.9 g buoyant weight year-1, representing a 250% difference. CCA play an important role in the growth and stabilization of carbonate reefs, so future changes of this magnitude could greatly impact coral reefs throughout the world. Coral calcification decreased between 15% and 20% under acidified conditions. Linear extension decreased by 14% under acidified conditions in one experiment. Larvae of the coral Pocillopora damicornis were able to recruit under the acidified conditions. In addition, there was no significant difference in production of gametes by the coral Montipora capitata after 6 months of exposure to the treatments. ?? 2008 Springer-Verlag.

  20. Quantification by optical coherence tomography imaging of the ablation volume obtained with the Orbital Atherectomy System in calcified coronary lesions

    NARCIS (Netherlands)

    Sotomi, Yohei; Cavalcante, Rafael; Shlofmitz, Richard A.; Suwannasom, Pannipa; Tateishi, Hiroki; Tenekecioglu, Erhan; Zheng, Yaping; Abdelghani, Mohammad; de Winter, Robbert J.; Wykrzykowska, Joanna J.; Onuma, Yoshinobu; Serruys, Patrick W.

    2016-01-01

    Recently, favourable procedural 30-day and one-year outcomes with the Diamondback 360 Orbital Atherectomy System (OAS) in the treatment of severely calcified lesions have been reported. The purpose of this study was to assess the therapeutic mechanism and efficacy of the OAS with optical coherence

  1. Mineralogical characterization of historical portuguese wall tiles of Sao Luis, Maranhao, Brazil; Caracterizacao mineralogica de azulejos portugueses do Centro Historico de Sao Luis do Maranhao

    Energy Technology Data Exchange (ETDEWEB)

    Rivas Mercury, J.M.; Vasconcelos, N.S.L.S.; Cabral, A.A., E-mail: rivascefetma@gmail.co [Instituto Federal de Educacao, Ciencia e Tecnologia do Maranhao (IFMA), Sao Luis, MA (Brazil). Programa de Mestrado em Engenharia de Materiais; Pereira, D.J. Costa [Centro de Criatividade Odylo Costa Filho (SECMA), Sao Luis, MA (Brazil). Centro Historico; Angelica, R.S. [Universidade Federal do Para (CG/UFPA), Belem, PA (Brazil). Inst. de Geociencias

    2010-07-01

    Portuguese wall tiles from centuries, XVII, XVIII and XIX, found in Sao Luis Maranhao has been studied by X-ray diffraction, in order to interpret the possible raw material and burning temperature. The mineral phases, Quartz, Wollastonite, Calcite and Gehlenite were identified in all samples. Based on the results it is possible to affirm that the main raw materials used to manufacture this materials was probably mixtures of kaolinite clay and calcite. Based on the mineralogical information it was also possible to state that burning temperature of this wall tiles was lower than 1000 deg C. (author)

  2. Alterações cognitivas na SAOS Cognitive impairment in obstructive sleep apnea syndrome

    Directory of Open Access Journals (Sweden)

    Pedro Felipe Carvalhedo de Bruin

    2010-06-01

    Full Text Available Alterações da cognição e do desempenho estão bem estabelecidas em pacientes com SAOS, causando um impacto significativo sobre a qualidade de vida e o risco de acidentes nesses indivíduos. Tais alterações são mais profundas nos quadros mais graves de SAOS, o que explica a aparente discrepância na frequência e gravidade desse prejuízo entre estudos com pacientes de clínicas de sono e estudos de base populacional. Vários aspectos podem estar comprometidos, incluindo o processamento cognitivo, a atenção sustentada, as funções executivas e a memória. Entretanto, os mecanismos causais desses déficits não estão inteiramente elucidados, e existem controvérsias, particularmente em relação à contribuição relativa da hipóxia intermitente e da interrupção do sono presentes na SAOS. O impacto da sonolência diurna sobre o desempenho desses pacientes nos diversos testes cognitivos também ainda deve ser determinado, assim como o possível efeito de comorbidades frequentes, incluindo o diabete melito, a hipertensão arterial sistêmica, a doença cardiovascular e a obesidade. Existem evidências convincentes de que o tratamento com CPAP produz uma significativa melhora do desempenho e da cognição, sobretudo nos portadores de SAOS moderada e grave, embora sejam necessários mais estudos acerca do seu impacto a longo prazo.Cognitive and performance impairment is well established in patients with obstructive sleep apnea syndrome (OSAS, having a significant impact on the quality of life and the risk of accidents in these individuals. The severity of the impairment correlates with that of the OSAS, which explains the apparent discrepancy between studies using patients from sleep clinics and population-based studies in terms of the reported frequency and severity of such impairment. Cognitive processing, sustained attention, executive functioning, and memory have all been reported to be impaired in OSAS. However, the causal

  3. Atmospheric element pollutant evaluation at the Sao Paulo University campus, Sao Paulo using Canoparmelia texana Lichen species

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, Rosiana R.; Saiki, Mitiko, E-mail: rosianarocha@yahoo.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2015-07-01

    The use of lichens as biomonitors of atmospheric pollutants has been considered as a very suitable tool when compared to conventional methods of direct measurements. Lichens in particular are widely used as biomonitors due to its easy sampling, low cost and resistance to environmental stresses. In this study, neutron activation analysis (NAA) was applied for element determinations in Canoparmelia texana lichenized fungi species. The samples were collected from tree barks in different sites at the Sao Paulo University Campus and in sites of areas considered non-polluted. Comparisons were made between the element concentration obtained in lichen from the study area and those from non-polluted sites. Results indicated that lichens from study area presented higher concentrations of As, Br, Ca, Cd, Co, Cr, Cs, Fe, K, La, Rb, Sb, Se and U than clean areas. The principal components analysis (PCA) was applied to the results obtained and five principal components were found as being responsible for almost 77 % of the variance. These findings suggest that element pollutants found may be associated with vehicular emissions, construction of buildings and metallurgical activities. (author)

  4. Atmospheric element pollutant evaluation at the Sao Paulo University campus, Sao Paulo using Canoparmelia texana Lichen species

    International Nuclear Information System (INIS)

    Rocha, Rosiana R.; Saiki, Mitiko

    2015-01-01

    The use of lichens as biomonitors of atmospheric pollutants has been considered as a very suitable tool when compared to conventional methods of direct measurements. Lichens in particular are widely used as biomonitors due to its easy sampling, low cost and resistance to environmental stresses. In this study, neutron activation analysis (NAA) was applied for element determinations in Canoparmelia texana lichenized fungi species. The samples were collected from tree barks in different sites at the Sao Paulo University Campus and in sites of areas considered non-polluted. Comparisons were made between the element concentration obtained in lichen from the study area and those from non-polluted sites. Results indicated that lichens from study area presented higher concentrations of As, Br, Ca, Cd, Co, Cr, Cs, Fe, K, La, Rb, Sb, Se and U than clean areas. The principal components analysis (PCA) was applied to the results obtained and five principal components were found as being responsible for almost 77 % of the variance. These findings suggest that element pollutants found may be associated with vehicular emissions, construction of buildings and metallurgical activities. (author)

  5. Cushing syndrome as presenting symptom of calcifying nested stromal-epithelial tumor of the liver in an adolescent boy: a case report

    NARCIS (Netherlands)

    Weeda, V. B.; de Reuver, Ph R.; Bras, H.; Zsíros, J.; Lamers, W. H.; Aronson, D. C.

    2016-01-01

    Ectopic adrenocorticotropic hormone-producing primary liver tumors are rare, especially in children. We report the case of an adolescent boy of mixed Dutch and Moroccan descent with an adrenocorticotropic hormone-producing calcifying nested stromal-epithelial tumor with long-term follow-up. Thus

  6. The Forkhead Transcription Factor FOXP2 Is Required for Regulation of p21WAF1/CIP1 in 143B Osteosarcoma Cell Growth Arrest.

    Science.gov (United States)

    Gascoyne, Duncan M; Spearman, Hayley; Lyne, Linden; Puliyadi, Rathi; Perez-Alcantara, Marta; Coulton, Les; Fisher, Simon E; Croucher, Peter I; Banham, Alison H

    2015-01-01

    Mutations of the forkhead transcription factor FOXP2 gene have been implicated in inherited speech-and-language disorders, and specific Foxp2 expression patterns in neuronal populations and neuronal phenotypes arising from Foxp2 disruption have been described. However, molecular functions of FOXP2 are not completely understood. Here we report a requirement for FOXP2 in growth arrest of the osteosarcoma cell line 143B. We observed endogenous expression of this transcription factor both transiently in normally developing murine osteoblasts and constitutively in human SAOS-2 osteosarcoma cells blocked in early osteoblast development. Critically, we demonstrate that in 143B osteosarcoma cells with minimal endogenous expression, FOXP2 induced by growth arrest is required for up-regulation of p21WAF1/CIP1. Upon growth factor withdrawal, FOXP2 induction occurs rapidly and precedes p21WAF1/CIP1 activation. Additionally, FOXP2 expression could be induced by MAPK pathway inhibition in growth-arrested 143B cells, but not in traditional cell line models of osteoblast differentiation (MG-63, C2C12, MC3T3-E1). Our data are consistent with a model in which transient upregulation of Foxp2 in pre-osteoblast mesenchymal cells regulates a p21-dependent growth arrest checkpoint, which may have implications for normal mesenchymal and osteosarcoma biology.

  7. Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Sophie Portron

    2015-01-01

    Full Text Available Background/Aims: Multipotent stem/stromal cells (MSC are considered promising for cartilage tissue engineering. However, chondrogenic differentiation of MSC can ultimately lead to the formation of hypertrophic chondrocytes responsible for the calcification of cartilage. To prevent the production of this calcified matrix at the articular site, the late hypertrophic differentiation of MSCs must be carefully controlled. Given that articular cartilage is avascular, we hypothesized that in addition to its stimulatory role in the early differentiation of chondrogenic cells, hypoxia may prevent their late hypertrophic conversion. Methods: Early and late chondrogenic differentiation were evaluated using human adipose MSC and murine ATDC5 cells cultured under either normoxic (21%O2 or hypoxic (5%O2 conditions. To investigate the effect of hypoxia on late chondrogenic differentiation, the transcriptional activity of hypoxia-inducible factor-1alpha (HIF-1α and HIF-2α were evaluated using the NoShift DNA-binding assay and through modulation of their activity (chemical inhibitor, RNA interference. Results: Our data demonstrate that low oxygen tension not only stimulates the early chondrogenic commitment of two complementary models of chondrogenic cells, but also inhibits their hypertrophic differentiation. Conclusion: These results suggest that hypoxia can be used as an instrumental tool to prevent the formation of a calcified matrix in MSC-based cartilage tissue engineering.

  8. Geology and petrology of alkaline Massif from Ilha de Vitoria, Sao Paulo State

    International Nuclear Information System (INIS)

    Motoki, A.

    1986-01-01

    Geological and petrological studies of the Vitoria Island Alkaline Complex, State of Sao Paulo, have been carried out by means of photo interpretation; field work, thin section studies, whole-rock chemical analysis, x-ray diffractometry, EPMA mineral analysis, and K-Ar and Rb-Sr dating. Radiometric dating indicates a late Cretaceous age for the Vitoria Island Alkaline Complex, which is concordant with the ages of other neighbouring alkaline bodies. (author)

  9. Lumen and calcium characteristics within calcified coronary lesions. Comparison of computed tomography coronary angiography versus intravascular ultrasound

    OpenAIRE

    Noll, Dariusz; Kruk, Mariusz; Pręgowski, Jerzy; Kaczmarska, Edyta; Kryczka, Karolina; Pracoń, Radosław; Skwarek, Mirosław; Dzielińska, Zofia; Petryka, Joanna; Śpiewak, Mateusz; Lubiszewska, Barbara; Norwa-Otto, Bożena; Opolski, Maksymilian; Witkowski, Adam; Demkow, Marcin

    2013-01-01

    Introduction Computed tomography coronary angiography (CTCA) is a diagnostic method used for exclusion of coronary artery disease. However, lower accuracy of CTCA in assessment of calcified lesions is a significant factor impeding applicability of CTCA for assessment of coronary atherosclerosis. Aim To provide insight into lumen and calcium characteristics assessed with CTCA, we compared these parameters to the reference of intravascular ultrasound (IVUS). Material and methods Two hundred and...

  10. Deuterium and oxygen-18 concentrations in rain and river waters from Minas Gerais, Sao Paulo and Parana States, Brazil; Concentracoes de deuterio e oxigenio-18 nas aguas de precipitacao e de superficie dos Estados de Minas Gerais, Sao Paulo e Parana

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Oliveiro J [Escola Superior de Agricultura de Lavras, MG (Brazil); Salati, Eneas [Sao Paulo Univ., Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz

    1982-07-01

    The main object of this paper was to study the concentration of deuterium (D) and Oxygen-18 ({sup 18} O) in the rain and river waters from the States of Minas Gerais, Sao paulo and Parana. Monthly samples were prepared and analysed at the Centro de Energia Nuclear na Agricultura (CENA-ESALQ-USP) with the use of CH-4 ({sup 18} O) and GD-150 (D) mass spectrometers of Varian-Mat. In order to correlate the D and {sup 18} O of the rain water the equation {delta}D = 9.22 + 7.17{delta}18 was found. Considering the three equations separately it was observed that the parameter a = {delta}D -b{delta}18 increases from the South to the North. For the Piracicaba River (city of Piracicaba only), the D and {sup 18} O relationship was expressed by {delta}D = -10.98 + 4.88{delta}18. The States of Minas Gerais and Sao Paulo can be divided according to the weight-average concentrations ({delta}18 m%{sub 0}). This division is not possible from Parana. Considering the average from the weight average concentration (annually and summer) for the three States, the annual average (D and {sup 18} O) is slightly richer than the summer average, showing the importance of the amount effect, mainly for the States of Minas Gerais and Sao Paulo. The rivers studied had little variation in the {sup 18} O concentrations with no definite periodicity. The rivers from the North of Minas Gerais are slightly richer in {sup 18} O than the rivers from the South. The waters from Rio Grande in the South of Minas Gerais State are poorer in {sup 18} O than the water of the same river in the `Triangle`region. The {delta}18% {sub 0} data for the rivers Grande, Parnaiba and Parana are very similar, so there is no way to compute the contribution of the first and the second rivers in the formation of the third. (author). 20 refs., 2 figs., 8 tabs.

  11. Geology and geochronology of Cardoso Island, in the southeastern coast of Sao Paulo State

    International Nuclear Information System (INIS)

    Weber, Werner

    1998-01-01

    This aim of work is the geological and geochronological study of rocks cropping out on Cardoso Island, on the southeastern coast of Sao Paulo States, close to be boundary with Parana State. The Island with an area of about 151 km 2 is a protected area administered by the Forest Institute of Environment Secretariat of the State of Sao Paulo. It is mountanious, with a peak at 814 m, and is covered by dense Mata Atlantica vegetation. The terrains which compose the island are mainly an igneous complex with light grey leucocratic, inequigranular, medium - to coarse-grained syenites. The predominant Tres Irmaos Syenite (STI), composed of pyroxene, hornblende, and perthitic to mesoperthitic microcline, has a magmatic flow structures, and is cut by the Cambriu alkali-feldspar Granites (GC), which is pinkish grey, leucocratic and medium-grained. Geochemical analysis of STI and GC demonstrate their meta luminous alkaline nature and late-orogenic to an orogenic character. The geochronological results suggest that the bodies were formed between 620 and 570 My according to the U-Pb method in zircons, with cooling between 597 and 531 My (K-Ar in amphiboles). Whole rock Sm-Nd analysis yield T DM ages in the Meso and Paleoproterozoic (1.200 - 2.200 My). belt of low grade meta sedimentary rocks occurs in the northern part of the island. Quartz schist, quartz-mica schist and mica-quartz schist, often containing andaluzite and cordierite, predominate. The geochemical and geochronological data suggest that the sources of the metasediments were andesites of continental arc whose protolities separated from the mantle during the Paleoproterozoic, between 1.800 and 2.200 My. These metasediments probably continue on the continent in the Taquari region and extend southwards in narrow strips between the granitoids of the Paranagua Domain. Although quaternary deposits are expressive, they were not studied in details since they were not the objectives of this study. (author)

  12. Analysis of the movement of calcified lymph nodes during breathing

    International Nuclear Information System (INIS)

    Jenkins, Peter; Salmon, Clare; Mannion, Cathy

    2005-01-01

    Purpose: To identify and measure the respiratory-induced movement of calcified mediastinal lymph nodes. Methods and materials: Twenty-one patients receiving radiation therapy for primary lung or pleural tumors were noted to have calcification within one or more mediastinal lymph nodes. The breathing motion of 27 such nodes was measured with orthogonal fluoroscopic imaging during quiet respiration. Results: All 27 nodes showed some motion synchronous with breathing. The mean respiratory movement was 6.6 mm, 2.6 mm, and 1.4 mm in the craniocaudal, dorsoventral, and mediolateral planes, respectively. There was a significant difference in the amplitude of motion in the craniocaudal plane compared with movement in the other two directions (p < 0.001). No differences were seen in the movement of lymph nodes dependent on position within the mediastinum (supracarinal vs. infracarinal or hilar vs. mediastinal). Neither size of the primary tumor nor spirometric parameters were correlated with the amplitude of lymph node movement. Conclusions: Mediastinal lymph nodes move during breathing, and this needs to be accounted for when the internal margin component of the PTV is defined. The amplitude of this movement is anisotropic and seems to be less than that reported for primary lung tumors. This should permit a modest reduction in the margin allowed for breathing movement around involved mediastinal nodes, particularly in the mediolateral and dorsoventral planes

  13. Measurements of Po214 at Sao Jose dos Campos and Cachoeira Paulista: correlation with meteorological data

    International Nuclear Information System (INIS)

    Marinho, E.V.A.; Nordemann, D.J.R.

    1985-01-01

    PO 214 , a short life daughter product of RN 222 , was measured by alpha-ray spectrometry of a atmospheric particles sampled on Millipore filters (0.8 μm pore) at Sao Jose dos Campos (23 0 12'S, 45 0 51'W) and Cachoeira Paulista (22 0 41'S, 45 0 00'W). The infleunce of rainfall on the atmospheric Po 214 was studied at Sao Jose dos Campos. The mean activity was 98 pCi/m 3 for dry days (without rain during the preceding two days). On the contrary a low mean activity of 35 pCi/m 3 was observed for high pluviometry days from 08/30 to 10/26 for which total rainfall was 365.5 mm. At Cachoeria Paulista the Po 214 activity was correlated with the stability of the air (measured by the Richardson number), showing an accumaltion of natural radioactive aerosols during periods of higher stability of the lower atmosphere. (author) [pt

  14. Single cell analysis of caspase-3 in apoptotic and non-apoptotic cells during mouse limb development

    Czech Academy of Sciences Publication Activity Database

    Adamová, Eva; Klepárník, Karel; Matalová, E.

    2014-01-01

    Roč. 3, - (2014), PP58 ISSN 2052-1219. [European Calcified Tissue Society Congress /41./. 17.05.2014-20.05.2014, Praha] R&D Projects: GA ČR GAP206/11/2377; GA ČR(CZ) GA14-28254S Institutional support: RVO:68081715 Keywords : single cell analysis * caspase-3 * mouse limb development Subject RIV: CB - Analytical Chemistry, Separation

  15. Computerized method for evaluating diagnostic image quality of calcified plaque images in cardiac CT: Validation on a physical dynamic cardiac phantom

    International Nuclear Information System (INIS)

    King, Martin; Rodgers, Zachary; Giger, Maryellen L.; Bardo, Dianna M. E.; Patel, Amit R.

    2010-01-01

    Purpose: In cardiac computed tomography (CT), important clinical indices, such as the coronary calcium score and the percentage of coronary artery stenosis, are often adversely affected by motion artifacts. As a result, the expert observer must decide whether or not to use these indices during image interpretation. Computerized methods potentially can be used to assist in these decisions. In a previous study, an artificial neural network (ANN) regression model provided assessability (image quality) indices of calcified plaque images from the software NCAT phantom that were highly agreeable with those provided by expert observers. The method predicted assessability indices based on computer-extracted features of the plaque. In the current study, the ANN-predicted assessability indices were used to identify calcified plaque images with diagnostic calcium scores (based on mass) from a physical dynamic cardiac phantom. The basic assumption was that better quality images were associated with more accurate calcium scores. Methods: A 64-channel CT scanner was used to obtain 500 calcified plaque images from a physical dynamic cardiac phantom at different heart rates, cardiac phases, and plaque locations. Two expert observers independently provided separate sets of assessability indices for each of these images. Separate sets of ANN-predicted assessability indices tailored to each observer were then generated within the framework of a bootstrap resampling scheme. For each resampling iteration, the absolute calcium score error between the calcium scores of the motion-contaminated plaque image and its corresponding stationary image served as the ground truth in terms of indicating images with diagnostic calcium scores. The performances of the ANN-predicted and observer-assigned indices in identifying images with diagnostic calcium scores were then evaluated using ROC analysis. Results: Assessability indices provided by the first observer and the corresponding ANN performed

  16. Thermal modeling and geomorphology of the south border of the Sao Francisco Craton: thermochronology by fission tracks in apatites;Modelagem termica e geomorfologia da borda sul do Craton do Sao Francisco: termocronologia por tracos de fissao em apatita

    Energy Technology Data Exchange (ETDEWEB)

    Hackspacher, Peter Christian [UNESP, Rio Claro, SP (Brazil). Inst. de Geociencias e Ciencias Exatas; Godoy, Daniel Francoso de; Franco, Ana Olivia Barufi [UNESP, Rio Claro, SP (Brazil). Pos-Graduacao em Geologia Regional; Ribeiro, Luiz Felipe Brandini [NUCLEARGEO, Rio Claro, SP (Brazil); Hadler Neto, Julio Cesar [Universidade Estadual de Campinas (IFGW/UNICAMP), SP (Brazil). Inst. de Fisica Gleb Wataghin

    2007-12-15

    Recent developments in Fission Track thermochronology associated to mesozoic-cenozoic erosion and tectonic presented trough thematic maps (isotemperature), permit to model the landscape evolution in the southern border of the Sao Francisco craton, southeastern Brazil. Paleotemperature, obtained by fission track analysis in apatite, is closely related to geomorphologic interpretations. The area suffered a complex imprint of endogenous and exogenous processes resulting diversified and differentiated relieves. The landscape is strongly controlled by exhumation between Jurassic and Lower Cretaceous, uplift with tectonic denudation related to crustal heating at the Upper Cretaceous and reactivation of faults until the Miocene. This scenario is a result of reactivations of different brittle structures that accommodate the deformation in the southern border of the Sao Francisco craton. The landscape reflects denudations of up to 3 km with preserved remains of erosive surfaces in the topographical tops and chronocorrelates deposits in the basins of the region. (author)

  17. Calcified cartilage or bone? Collagens in the tessellated endoskeletons of cartilaginous fish (sharks and rays).

    Science.gov (United States)

    Seidel, Ronald; Blumer, Michael; Pechriggl, Elisabeth-Judith; Lyons, Kady; Hall, Brian K; Fratzl, Peter; Weaver, James C; Dean, Mason N

    2017-10-01

    The primary skeletal tissue in elasmobranchs -sharks, rays and relatives- is cartilage, forming both embryonic and adult endoskeletons. Only the skeletal surface calcifies, exhibiting mineralized tiles (tesserae) sandwiched between a cartilage core and overlying fibrous perichondrium. These two tissues are based on different collagens (Coll II and I, respectively), fueling a long-standing debate as to whether tesserae are more like calcified cartilage or bone (Coll 1-based) in their matrix composition. We demonstrate that stingray (Urobatis halleri) tesserae are bipartite, having an upper Coll I-based 'cap' that merges into a lower Coll II-based 'body' zone, although tesserae are surrounded by cartilage. We identify a 'supratesseral' unmineralized cartilage layer, between tesserae and perichondrium, distinguished from the cartilage core in containing Coll I and X (a common marker for mammalian mineralization), in addition to Coll II. Chondrocytes within tesserae appear intact and sit in lacunae filled with Coll II-based matrix, suggesting tesserae originate in cartilage, despite comprising a diversity of collagens. Intertesseral joints are also complex in their collagenous composition, being similar to supratesseral cartilage closer to the perichondrium, but containing unidentified fibrils nearer the cartilage core. Our results indicate a unique potential for tessellated cartilage in skeletal biology research, since it lacks features believed diagnostic for vertebrate cartilage mineralization (e.g. hypertrophic and apoptotic chondrocytes), while offering morphologies amenable for investigating the regulation of complex mineralized ultrastructure and tissues patterned on multiple collagens. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell

    Science.gov (United States)

    2009-01-01

    Background Eukaryotes are classified as either haplontic, diplontic, or haplo-diplontic, depending on which ploidy levels undergo mitotic cell division in the life cycle. Emiliania huxleyi is one of the most abundant phytoplankton species in the ocean, playing an important role in global carbon fluxes, and represents haptophytes, an enigmatic group of unicellular organisms that diverged early in eukaryotic evolution. This species is haplo-diplontic. Little is known about the haploid cells, but they have been hypothesized to allow persistence of the species between the yearly blooms of diploid cells. We sequenced over 38,000 expressed sequence tags from haploid and diploid E. huxleyi normalized cDNA libraries to identify genes involved in important processes specific to each life phase (2N calcification or 1N motility), and to better understand the haploid phase of this prominent haplo-diplontic organism. Results The haploid and diploid transcriptomes showed a dramatic differentiation, with approximately 20% greater transcriptome richness in diploid cells than in haploid cells and only ≤ 50% of transcripts estimated to be common between the two phases. The major functional category of transcripts differentiating haploids included signal transduction and motility genes. Diploid-specific transcripts included Ca2+, H+, and HCO3- pumps. Potential factors differentiating the transcriptomes included haploid-specific Myb transcription factor homologs and an unusual diploid-specific histone H4 homolog. Conclusions This study permitted the identification of genes likely involved in diploid-specific biomineralization, haploid-specific motility, and transcriptional control. Greater transcriptome richness in diploid cells suggests they may be more versatile for exploiting a diversity of rich environments whereas haploid cells are intrinsically more streamlined. PMID:19832986

  19. Indoor Particulate Matter in Houses of Elderly in the Metropolitan Area of Sao Paulo, Brazil

    Science.gov (United States)

    Segalin, B.; Goncalves, F. T.; Fornaro, A.

    2015-12-01

    Environmental Company of the State of Sao Paulo (CETESB), Brazil, is responsible for particulate matter measurements (PM) in the Metropolitan Area of Sao Paulo (MASP). However, there are few works with indoor measures for MASP. Therefore, the aim of this work is to investigate the PM in households in the MASP. The chosen households were there are aged people over 60 years old. The measurements were sampled during 24 hours using a Personal Cascade Impactor (SKC Cat No. 225-370), which the following aerodynamic diameters: 10.0 - 2.5 (A); 1.0 - 2.5 (B); 0.50 - 1.0 (C); 0.25 - 0.50 (D), and < 0.25 μm (E). Together the impactor, there is a Leland Legacy pump (SKC Cat No. 100-3002) with a flow of 9L/min. It was analyzed 56 households with average values of PM10 and PM2.5 of 30.7 and 23.4 μg/m3, respectively. On average, 76% of PM10 consists of PM2.5, percentage higher than the outdoor environment (60% - CETESB), and 43% of the PM2.5 consists of PM smaller than 0.25 μm. Among all households, there was no exceedance of thresholds national standards PM10 (120 μg/m3) and PM2.5 (60 μg/m3). However, 10.7% of residences exceeded the PM10 threshold of the World Health Organization (50 μg/m3) and 39.2% for PM2.5 (20 μg/m3). The cluster analysis grouped the measures in the houses in four profiles. In three of them were greater amount of mass in ultrafine particles (E), followed by coarse particles (A) with the minimum in C level. The maximum in E may be due to the high contribution vehicular and secondary aerosol outdoor environment. The secondary maximum in A may be due to particles ressuspension and also arising from outdoors. These three groups differ only by the amount of PM measured in the households; they represent high, medium and low PM concentrations. The fourth group has average concentrations, but it presents a different profile because its maximum is in the D rather than E. All data will be analyzed concerning the possible sources.

  20. Dental calculus: the calcified biofilm and its role in disease development.

    Science.gov (United States)

    Akcalı, Aliye; Lang, Niklaus P

    2018-02-01

    Dental calculus represents the first fossilized record of bacterial communities as a testimony of evolutionary biology. The development of dental calculus is a dynamic process that starts with a nonmineralized biofilm which eventually calcifies. Nonmineralized dental biofilm entraps particles from the oral cavity, including large amounts of oral bacteria, human proteins, viruses and food remnants, and preserves their DNA. The process of mineralization involves metabolic activities of the bacterial colonies and strengthens the attachment of nonmineralized biofilms to the tooth surface. From a clinical point of view, dental calculus always harbors a living, nonmineralized biofilm, jeopardizing the integrity of the dento-gingival or implanto-mucosal unit. This narrative review presents a brief historical overview of dental calculus formation and its clinical relevance in modern periodontal practice. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. Shaded Relief of Rio Sao Francisco, Brazil

    Science.gov (United States)

    2000-01-01

    This topographic image acquired by SRTM shows an area south of the Sao Francisco River in Brazil. The scrub forest terrain shows relief of about 400 meters (1300 feet). Areas such as these are difficult to map by traditional methods because of frequent cloud cover and local inaccessibility. This region has little topographic relief, but even subtle changes in topography have far-reaching effects on regional ecosystems. The image covers an area of 57 km x 79 km and represents one quarter of the 225 km SRTM swath. Colors range from dark blue at water level to white and brown at hill tops. The terrain features that are clearly visible in this image include tributaries of the Sao Francisco, the dark-blue branch-like features visible from top right to bottom left, and on the left edge of the image, and hills rising up from the valley floor. The San Francisco River is a major source of water for irrigation and hydroelectric power. Mapping such regions will allow scientists to better understand the relationships between flooding cycles, forestation and human influences on ecosystems.This shaded relief image was generated using topographic data from the Shuttle Radar Topography Mission. A computer-generated artificial light source illuminates the elevation data to produce a pattern of light and shadows. Slopes facing the light appear bright, while those facing away are shaded. On flatter surfaces, the pattern of light and shadows can reveal subtle features in the terrain. Shaded relief maps are commonly used in applications such as geologic mapping and land use planning.The Shuttle Radar Topography Mission (SRTM), launched on February 11, 2000, uses the same radar instrument that comprised the Spaceborne Imaging Radar-C/X-Band Synthetic Aperture Radar (SIR-C/X-SAR) that flew twice on the Space Shuttle Endeavour in 1994. The mission is designed to collect three-dimensional measurements of the Earth's surface. To collect the 3-D data, engineers added a 60-meter-long (200

  2. Search for microbial signatures within human and microbial calcifications using soft x-ray spectromicroscopy.

    Science.gov (United States)

    Benzerara, Karim; Miller, Virginia M; Barell, Gerard; Kumar, Vivek; Miot, Jennyfer; Brown, Gordon E; Lieske, John C

    2006-11-01

    The origin of advanced arterial and renal calcification remains poorly understood. Self-replicating, calcifying entities have been detected and isolated from calcified human tissues, including blood vessels and kidney stones, and are referred to as nanobacteria. However, the microbiologic nature of putative nanobacteria continues to be debated, in part because of the difficulty in discriminating biomineralized microbes from minerals nucleated on anything else (eg, macromolecules, cell membranes). To address this controversy, the use of techniques capable of characterizing the organic and mineral content of these self-replicated structures at the submicrometer scale would be beneficial. Calcifying gram-negative bacteria (Caulobacter crescentus, Ramlibacter tataouinensis) used as references and self-replicating calcified nanoparticles cultured from human samples of calcified aneurysms were examined using a scanning transmission x-ray microscope (STXM) at the Advanced Light Source at Lawrence Berkeley National Laboratory. This microscope uses a monochromated and focused synchrotron x-ray beam (80-2,200 eV) to yield microscopic and spectroscopic information on both organic compounds and minerals at the 25 nm scale. High-spatial and energy resolution near-edge x-ray absorption fine structure (NEXAFS) spectra indicative of elemental speciation acquired at the C K-edge, N K-edge, and Ca L(2,3)-edge on a single-cell scale from calcified C. crescentus and R. tataouinensis displayed unique spectral signatures different from that of nonbiologic hydroxyapatite (Ca(10)(PO(4))(6)(OH)(2)). Further, preliminary NEXAFS measurements of calcium, carbon, and nitrogen functional groups of cultured calcified nanoparticles from humans revealed evidence of organics, likely peptides or proteins, specifically associated with hydroxyapatite minerals. Using NEXAFS at the 25 nm spatial scale, it is possible to define a biochemical signature for cultured calcified bacteria, including proteins

  3. The role of calcified cartilage and subchondral bone in the initiation and progression of ochronotic arthropathy in alkaptonuria.

    Science.gov (United States)

    Taylor, A M; Boyde, A; Wilson, P J M; Jarvis, J C; Davidson, J S; Hunt, J A; Ranganath, L R; Gallagher, J A

    2011-12-01

    Alkaptonuria is a genetic disorder of tyrosine metabolism, resulting in elevated circulating concentrations of homogentisic acid. Homogentisic acid is deposited as a polymer, termed ochronotic pigment, in collagenous tissues, especially cartilages of weight-bearing joints, leading to a severe osteoarthropathy. We undertook this study to investigate the initiation and progression of ochronosis from the earliest detection of pigment through complete joint failure. Nine joint samples with varying severities of ochronosis were obtained from alkaptonuria patients undergoing surgery and compared to joint samples obtained from osteoarthritis (OA) patients. Samples were analyzed by light and fluorescence microscopy, 3-dimensional scanning electron microscopy (SEM), and the quantitative backscattered electron mode of SEM. Cartilage samples were mechanically tested by compression to determine Young's modulus of pigmented, nonpigmented, and OA cartilage samples. In alkaptonuria samples with the least advanced ochronosis, pigment was observed intracellularly and in the territorial matrix of individual chondrocytes at the boundary of the subchondral bone and calcified cartilage. In more advanced ochronosis, pigmentation was widespread throughout the hyaline cartilage in either granular composition or as blanket pigmentation in which there is complete and homogenous pigmentation of cartilage matrix. Once hyaline cartilage was extensively pigmented, there was aggressive osteoclastic resorption of the subchondral plate. Pigmented cartilage became impacted on less highly mineralized trabeculae and embedded in the marrow space. Pigmented cartilage samples were much stiffer than nonpigmented or OA cartilage as revealed by a significant difference in Young's modulus. Using alkaptonuria cartilage specimens with a wide spectrum of pigmentation, we have characterized the progression of ochronosis. Intact cartilage appears to be resistant to pigmentation but becomes susceptible following

  4. Preclinical Testing of an Oncolytic Parvovirus: Standard Protoparvovirus H-1PV Efficiently Induces Osteosarcoma Cell Lysis In Vitro

    OpenAIRE

    Carsten Geiss; Zoltán Kis; Barbara Leuchs; Monika Frank-Stöhr; Jörg R. Schlehofer; Jean Rommelaere; Christiane Dinsart; Jeannine Lacroix

    2017-01-01

    Osteosarcoma is the most frequent malignant disease of the bone. On the basis of early clinical experience in the 1960s with H-1 protoparvovirus (H-1PV) in osteosarcoma patients, this effective oncolytic virus was selected for systematic preclinical testing on various osteosarcoma cell cultures. A panel of five human osteosarcoma cell lines (CAL 72, H-OS, MG-63, SaOS-2, U-2OS) was tested. Virus oncoselectivity was confirmed by infecting non-malignant human neonatal fibroblasts and osteoblasts...

  5. Br-rich tips of calcified crab claws are less hard but more fracture resistant: a comparison of mineralized and heavy-element biological materials.

    Science.gov (United States)

    Schofield, Robert M S; Niedbala, Jack C; Nesson, Michael H; Tao, Ye; Shokes, Jacob E; Scott, Robert A; Latimer, Matthew J

    2009-06-01

    We find that the spoon-like tips of the chelipeds (large claws) of the crab Pachygrapsus crassipes differ from the rest of the claw in that they are not calcified, but instead contain about 1% bromine--thus they represent a new example of a class of structural biological materials that contain heavy elements such as Zn, Mn, Fe, Cu, and Br bound in an organic matrix. X-ray absorption spectroscopy data suggest that the bromine is bound to phenyl rings, possibly in tyrosine. We measure a broad array of mechanical properties of a heavy-element biological material for the first time (abrasion resistance, coefficient of kinetic friction, energy of fracture, hardness, modulus of elasticity and dynamic mechanical properties), and we make a direct comparison with a mineralized tissue. Our results suggest that the greatest advantage of bromine-rich cuticle over calcified cuticle is resistance to fracture (the energy of fracture is about an order of magnitude greater than for calcified cuticle). The greatest advantage relative to unenriched cuticle, represented by ant mandible cuticle, is a factor of about 1.5 greater hardness and modulus of elasticity.The spoon-like tips gain additional fracture resistance from the orientation of the constituent laminae and from the viscoelasticity of the material. We suggest that fracture resistance is of greater importance in smaller organisms, and we speculate that one function of heavy elements in structural biological materials is to reduce molecular resonant frequencies and thereby increase absorption of energy from impacts.

  6. Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

    Directory of Open Access Journals (Sweden)

    Liskova J

    2015-01-01

    Full Text Available Jana Liskova,1 Oleg Babchenko,2 Marian Varga,2 Alexander Kromka,2 Daniel Hadraba,1 Zdenek Svindrych,1 Zuzana Burdikova,1 Lucie Bacakova1 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 2Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic Abstract: Nanocrystalline diamond (NCD films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination or oxygen atoms (O-termination. Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix

  7. Conception of the Instrument Calibration Laboratory of Ionizing Radiation Measurement (LACIMRI) of CTMSP - Sao Paulo, SP

    International Nuclear Information System (INIS)

    Silva, Raimundo Dias da; Kibrit, Eduardo

    2009-01-01

    The present work describes the phases of implantation of calibration laboratory of ionizing radiation measurement instruments at the CTMSP, Sao Paulo, in a priory approved by CNEN, Brazil. That laboratory will allow and enhance the present metrological capacity for the attendance to the growing demand for calibration services of the instruments

  8. Farnesyl diphosphate synthase is involved in the resistance to zoledronic acid of osteosarcoma cells. : resistance of osteosarcoma to nitrogen bisphosphonates

    OpenAIRE

    Ory , Benjamin; Moriceau , Gatien; Trichet , Valérie; Blanchard , Frédéric; Berreur , Martine; Rédini , Françoise; Rogers , Michael; Heymann , Dominique

    2008-01-01

    International audience; We recently demonstrated original anti-tumor effects of zoledronic acid (Zol) on osteosarcoma cell lines independently of their p53 and Rb status. The present study investigated the potential Zol-resistance acquired by osteosarcoma cells after prolonged treatment. After 12 weeks of culture in the presence of 1 microm Zol, the effects of high doses of Zol (10-100 microm) were compared between the untreated rat (OSRGA, ROS) and human (MG63, SAOS2) osteosarcoma cells and ...

  9. Cells responding to surface structure of calcium phosphate ceramics for bone regeneration.

    Science.gov (United States)

    Zhang, Jingwei; Sun, Lanying; Luo, Xiaoman; Barbieri, Davide; de Bruijn, Joost D; van Blitterswijk, Clemens A; Moroni, Lorenzo; Yuan, Huipin

    2017-11-01

    Surface structure largely affects the inductive bone-forming potential of calcium phosphate (CaP) ceramics in ectopic sites and bone regeneration in critical-sized bone defects. Surface-dependent osteogenic differentiation of bone marrow stromal cells (BMSCs) partially explained the improved bone-forming ability of submicron surface structured CaP ceramics. In this study, we investigated the possible influence of surface structure on different bone-related cells, which may potentially participate in the process of improved bone formation in CaP ceramics. Besides BMSCs, the response of human brain vascular pericytes (HBVP), C2C12 (osteogenic inducible cells), MC3T3-E1 (osteogenic precursors), SV-HFO (pre-osteoblasts), MG63 (osteoblasts) and SAOS-2 (mature osteoblasts) to the surface structure was evaluated in terms of cell proliferation, osteogenic differentiation and gene expression. The cells were cultured on tricalcium phosphate (TCP) ceramics with either micron-scaled surface structure (TCP-B) or submicron-scaled surface structure (TCP-S) for up to 14 days, followed by DNA, alkaline phosphatase (ALP) and quantitative polymerase chain reaction gene assays. HBVP were not sensitive to surface structure with respect to cell proliferation and osteogenic differentiation, but had downregulated angiogenesis-related gene expression (i.e. vascular endothelial growth factor) on TCP-S. Without additional osteogenic inducing factors, submicron-scaled surface structure enhanced ALP activity and osteocalcin gene expression of human (h)BMSCs and C2C12 cells, favoured the proliferation of MC3T3-E1, MG63 and SAOS-2, and increased ALP activity of MC3T3-E1 and SV-HFO. The results herein indicate that cells with osteogenic potency (either osteogenic inducible cells or osteogenic cells) could be sensitive to surface structure and responded to osteoinductive submicron-structured CaP ceramics in cell proliferation, ALP production or osteogenic gene expression, which favour bone

  10. Conservation programs impact in the industrial energetic demand of the Sao Paulo State

    International Nuclear Information System (INIS)

    Silva Walter, A.C. da; Bajay, S.V.

    1987-01-01

    This paper describes the evaluation methodology of the impacts of conservation and substitution programs on the industrial energy demand in the State of Sao Paulo. The main industrial sectors are investigated. An econometric energy demand forecasting model is used to project the demand in the planning period. After an analysis of the conservation and substitution possibilities in each industrial sector, a correction in the projected demand is made through adoption of assumptions oriented by these studies. (author)

  11. Hydrochemical and isotopic study of the Botucatu aquifer ground waters in Sao Paulo State

    International Nuclear Information System (INIS)

    Silva, R.B.G. da.

    1983-01-01

    The process controlling chemical composition of ground water,its origin and apparent age as well as, the natural flow rate of the water in Botucatu aquifer in state of Sao Paulo, Brazil, have been investigated using hydrochemical and environmental isotopic ( 18 O, 2 H, 13 C, 14 C) Technics. The main recharge process is assumed to be the infiltration of rain water in the aquifer outcrop area. The progressive confining conditions with the increasing depth of the top of the aquifer layer makes the ground water temperature slowly greater. The recent magnesium and sodium bicarbonated waters changes first to sodium bicarbonated and then to sodium chloride-sulfated waters which are oldest found out in the research area(around 30.000 years ago). The mean Darcy permeability estimated on basis of 14 C and hydraulic gradient data is 2.6x10 -5 m/s. 9 maps (author) [pt

  12. Seasonal variations of 222Rn and SGD fluxes to Ubatuba embayments, Sao Paulo

    International Nuclear Information System (INIS)

    Oliveira, J.; Costa, P.; Braga, E.S.

    2006-01-01

    We describe here an application of excess 222 Rn to estimate submarine groundwater discharge in a series of small embayments of Ubatuba, Sao Paulo State, Brazil. Excess 222 Rn inventories obtained in 11 vertical profiles varied from (3.3±1.1) x 10 3 to (19±5) x 10 3 dpm x m -2 . The estimated total fluxes required to support the inventories varied from (0.6±0.2) x 10 3 to (3.4±0.9) x 10 3 dpm x m -2 x d -1 . Considering these results, the submarine groundwater discharge advective rates necessary to balance the sub-pycnocline fluxes calculated in Ubatuba embayments ranged from 0.06 to 1.9 cm x d -1 . During the period of this investigation (from March/03 to May/2004), the highest 222 Rn in excess inventories were observed late in the summer season (March). Taking into account all vertical profiles established, the relative variability was 67%. Although, if we consider only the fluxes determined in both Flamengo and Fortaleza embayments, the relative variation was 21%. (author)

  13. Total Diet Study. Mg and Mn content estimation of a Market Basket of Sao Paulo state (Brazil) by Instrumental Neutron Activation

    International Nuclear Information System (INIS)

    Roseane Pagliaro Avegliano; Vera Akiko Maihara

    2014-01-01

    Total Diet Studies (TDS) have been carried out to estimate dietary intakes of the essential and toxic elements for a large-scale population over a specific period of time. In this study, the TDS was based on the evaluation of food representing a Market Basket (MB), which reflected the dietary habits of the Sao Paulo State population, corresponding to 72 % of the average food consumption for the state of Sao Paulo. In the present Total Diet Study, magnesium and manganese concentrations were determined in 30 of the most consumed food groups of a MB of Sao Paulo State, Brazil. Instrumental Neutron Activation Analysis (INAA) has been successfully used on a regularly basis in several areas of nutrition and foodstuffs. Element concentrations were determined by INAA in freeze-dried samples and ranged in mg kg -1 . Mg 41.4 (fats)-5287 (coffee) and Mn 0.12 (prime grade beef)-32.9 (coffee). The average daily Mg and Mn intake was calculated by multiplying the concentration of each element in each table-ready food group by the respective weight (g day -1 ) of the food group in the MB and adding the products from all food groups. The results of daily dietary intakes in this study were: Mg 174.8 and Mn 1.34 mg day -1 . Theses values were lower than the adequate intake (AI) proposed by the Food and Nutrition Board of the Institute of Medicine (USA National Academy) for adults. The low levels of Mg and Mn intakes presented in this TDS are probably due to the fact that MB of this study represented only 72 % of the weight of the most consumed household foods of Sao Paulo State. (author)

  14. Methodology for correlations between doses and detectability in standard mammographic images: application in Sao Paulo state

    International Nuclear Information System (INIS)

    Furquim, Tania Aparecida Correia

    2005-01-01

    Measurements using mammography units were performed in loco in 50 health establishments, randomly sampled from an equipment list of the Cadastro Nacional de Estabelecimentos de Saude (Health Establishments Brazilian Catalog). For the measurements six phantoms were utilized to establish different quality criteria and to evaluate doses in different breast thicknesses. Two different methods of measuring average glandular doses (AGD) were applied, and measurements of entrance surface doses (ESD) were also realized, in order to obtain mean values to Sao Paulo State. A study relating distribution and properties of different mammography trademarks with doses was performed. The sensitometry of processors allowed a quantification of the film-processing contrast index, A g , establishing a state mean value. The phantom images allowed the evaluation of detection limits of structures as microcalcifications, fibers, and masses, and state mean values were established for: spatial resolution (on surface and glandular breast position); image contrast; and detection expert ability from phantom images in two situations: before knowing the image targets and after viewing of a target map. Then, the results were compared to target detections in laboratory environment. Based on dose results, A g , image contrast, maximum contrast, and detection ratio, a relationship between them was determined. The results show that, in Sao Paulo State, mean glandular doses were lower than reference levels considering the Wu method, and close to or above reference levels for ail phantoms considering the Dance method. The ESD was always close to or above reference levels. The A g presented a mean value of (10,42 ± 0,20) for Sao Paulo State, and the image contrast was lower than the required limits established by the phantom manufacturers. The high contrast resolution showed that mammography units presented the expected values of line pair per mm in the State. The detectability evaluation of local

  15. Deuterium and oxygen-18 concentrations in rain and river waters from Minas Gerais, Sao Paulo and Parana States, Brazil

    International Nuclear Information System (INIS)

    Vieira, Oliveiro J.; Salati, Eneas

    1982-01-01

    The main object of this paper was to study the concentration of deuterium (D) and Oxygen-18 ( 18 O) in the rain and river waters from the States of Minas Gerais, Sao paulo and Parana. Monthly samples were prepared and analysed at the Centro de Energia Nuclear na Agricultura (CENA-ESALQ-USP) with the use of CH-4 ( 18 O) and GD-150 (D) mass spectrometers of Varian-Mat. In order to correlate the D and 18 O of the rain water the equation δD = 9.22 + 7.17δ18 was found. Considering the three equations separately it was observed that the parameter a = δD -bδ18 increases from the South to the North. For the Piracicaba River (city of Piracicaba only), the D and 18 O relationship was expressed by δD = -10.98 + 4.88δ18. The States of Minas Gerais and Sao Paulo can be divided according to the weight-average concentrations (δ18 m% 0 ). This division is not possible from Parana. Considering the average from the weight average concentration (annually and summer) for the three States, the annual average (D and 18 O) is slightly richer than the summer average, showing the importance of the amount effect, mainly for the States of Minas Gerais and Sao Paulo. The rivers studied had little variation in the 18 O concentrations with no definite periodicity. The rivers from the North of Minas Gerais are slightly richer in 18 O than the rivers from the South. The waters from Rio Grande in the South of Minas Gerais State are poorer in 18 O than the water of the same river in the 'Triangle'region. The δ18% 0 data for the rivers Grande, Parnaiba and Parana are very similar, so there is no way to compute the contribution of the first and the second rivers in the formation of the third. (author). 20 refs., 2 figs., 8 tabs

  16. Scenario of distributed energetic resources in the state of Sao Paulo, Brazil; O cenario dos recursos energeticos distribuidos no estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Burani, Geraldo Francisco; Udaeta, Miguel Edgar Morales; Fujii, Ricardo Junqueira; Galvao, Luiz Claudio Ribeiro [Universidade de Sao Paulo (USP), SP (Brazil). Dept. de Engenharia de Energia e Automacao Eletricas. Grupo de Energia], e-mail: udaeta@pea.usp.br

    2004-07-01

    This paper aims to assess the viability of the use of distributed resources for power production inside the state of Sao Paulo, taking into account its different forms, advantages and drawbacks. To accomplish the assessment and using de integrated energy resources planning, the full cost accounting for energy resources was applied to each administrative region of the state, which allowed a greater focus without jeopardizing the final results. The analysis were performed considering the usage of the most prospective energy sources such as wind, solar, natural gas, hydro and biomass generation; the results show that when the different aspects of each type of distributed generation are taken into account the renewable forms of energy generation are likely to be more competitive, when compared to traditional forms of generation. (author)

  17. Cost-effectiveness analysis of orbital atherectomy plus balloon angioplasty vs balloon angioplasty alone in subjects with calcified femoropopliteal lesions.

    Science.gov (United States)

    Weinstock, Barry; Dattilo, Raymond; Diage, Tiffini

    2014-01-01

    As cost considerations become increasingly critical when selecting optimal endovascular treatment strategies, a cost-benefit analysis was conducted comparing the Diamondback 360°® Orbital Atherectomy System (OAS) (Cardiovascular Systems, Inc., St Paul, MN, USA) and balloon angioplasty (BA) vs BA alone for treatment of calcified femoropopliteal lesions. The clinical outcomes from COMPLIANCE 360°, a prospective, multicenter, randomized study comparing OAS+BA vs BA alone for treatment of calcified femoropopliteal lesions, were correlated with cost data and previously published quality of life data. Site of service, hospital charges, and associated medical resource utilization were obtained from Uniform Billing statements for index treatments and associated revascularizations out to 1 year. Hospital costs were estimated using hospital-specific, procedure-specific cost-to-charge ratios. Length of stay and procedural data were collected from participating study sites. Twenty-five subjects with 38 lesions and 25 subjects with 27 lesions were randomized to the OAS+BA and BA-alone groups, respectively. Mean hospital charges (US$51,755 vs US$39,922) and estimated hospital costs (US$15,100 vs US$11,016) were higher for OAS+BA compared with BA alone (not statistically significant). Stent utilization was statistically significantly higher with BA-alone treatment for all subjects (1.1 vs 0.1, P=0.001) and in the subset of subjects with one lesion (1.0 vs 0.1, Pcost for single-lesion versus multiple-lesion treatment. Using costs and quality-adjusted life years (QALYs) for the single-lesion cohort, the 1-year incremental cost of OAS+BA vs BA alone was US$549, and incremental QALY was 0.16. This results in an incremental cost-effectiveness ratio of US$3,441, well below the US$50,000 threshold. One-year index procedure cost and cost-effectiveness were comparable for OAS+BA vs BA alone. This study provides compelling cost-effectiveness data for using atherectomy for treatment of

  18. Calcified Cavitating Mesenteric Lymph Node Syndrome: Case Presentation and Literature Review

    Directory of Open Access Journals (Sweden)

    Dipinder S Keer

    2010-01-01

    Full Text Available Cavitating mesenteric lymph node syndrome (CMLNS is a rare complication of celiac disease. Globally, only 36 cases of CMLNS have been reported to date. The present article reports an incidence of its unique pathology and possible complications of celiac disease, followed by a review of the syndrome. A case involving a 51-year-old man with celiac disease who was referred to hospital because of a non-tender abdominal mass is described. Plain film x-ray of the abdomen revealed fine curvilinear calcifications in the left lower quadrant. A complex, cystic-appearing, lobulated mass with somewhat echogenic walls most consistent with calcifications was revealed on subsequent ultrasound (US imaging. Colour Doppler imaging showed no evidence of vascularity within the lesion. Computed tomography (CT imaging showed a thin rim of calcification in the walls of multiple cystic components. Enhanced magnetic resonance (MR imaging revealed a mixed solid and cystic multiloculated mass, with fat-fluid layers originating from the root of the small bowel mesentery. A CT-guided biopsy was performed. The fine-needle aspirate revealed calcified matter with no associated cellular material. No malignant cells were seen; CMLNS was established as the diagnosis. To the authors’ knowledge, there are no previous reports in the literature describing the finding of rim calcification on US or MR imaging in the setting of CMLNS. CMLNS is an important diagnosis to consider, particularly in patients with a history of celiac disease. The finding of rim calcification on US in the setting of cavitating mesenteric adenopathy should prompt further diagnostic imaging studies such as CT or MR imaging. These may lead to additional pathology studies such as a CT-guided biopsy to further characterize the lesion at the cellular level, to investigate potential malignancy and to further guide follow-up and patient management.

  19. The bacterial meningitis score to distinguish bacterial from aseptic meningitis in children from Sao Paulo, Brazil.

    Science.gov (United States)

    Mekitarian Filho, Eduardo; Horita, Sérgio Massaru; Gilio, Alfredo Elias; Alves, Anna Cláudia Dominguez; Nigrovic, Lise E

    2013-09-01

    In a retrospective cohort of 494 children with meningitis in Sao Paulo, Brazil, the Bacterial Meningitis Score identified all the children with bacterial meningitis (sensitivity 100%, 95% confidence interval: 92-100% and negative predictive value 100%, 95% confidence interval: 98-100%). Addition of cerebrospinal fluid lactate to the score did not improve clinical prediction rule performance.

  20. Application of a nuclear microprobe to the study of calcified tissues

    Science.gov (United States)

    Coote, Graeme E.; Vickridge, Ian C.

    1988-03-01

    The mineral fraction of calcified tissue is largely calcium hydroxyapatite (bones and teeth) or calcium carbonate (shells and fish otoliths). Apatite has such a strong affinity for fluoride ions that the F/Ca ratio can vary markedly with position in a bone or tooth, depending on the amount of fluoride present at the time of calcification or partial recrystallization. New biological information can be obtained by introducing extra fluoride into the diet of an animal and using a microprobe later to scan sections of bones or teeth. In suitable burial sites extra fluoride is introduced after death, and the new distribution may have applications in forensic science and archaeology. Fish otoliths are also of interest since a new carbonate layer is formed each day and the distribution of trace elements may record some aspects of the fish's life history. Results from the following studies are presented: fluorine distributions in the teeth of sheep which ingested extra fluoride for known periods; distributions of calcium and fluorine in femurs of rats which drank water high in fluoride for periods from 2 to 15 weeks; calcium and fluorine distributions in artificially-prepared lesions in tooth enamel; diffusion profiles in archaeological human teeth and animal bones; patterns in the strontium/calcium ratio in sectioned otoliths of several species of fish.

  1. Mercury and selenium accumulation assessment in fish most consumed by Cubatao Community, Sao Paulo, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Farias, Luciana A.; Favaro, Deborah I.T.; Curcho, Michel R.S.M.; Fonseca, Barbara C.; Nascimento, Soraia M., E-mail: lufarias2@yahoo.com.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), SP (Brazil). Lab. de Analise por Ativacao de Neutrons; Kunioshi, Leonardo; Braga, Elisabete S., E-mail: edsbraga@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. Oceanografico. Lab. de Nutrientes, Micronutrientes e Tracos nos Oceanos (LABNUT)

    2009-07-01

    High correlation between mercury (Hg) and selenium (Se) concentrations in fish organs is well known. In the present study, 58 fish samples of five fish species most commonly consumed by the Cubatao city population, Sao Paulo State, Brazil were analyzed. The Cubatao Estuary, located in southeastern Sao Paulo State, is an economically important area suffering severe environmental water quality problems due to industrial wastes, domestic sewage and solid residues. The waters of the bay suffer the impact of the immense industrial complex of Cubatao. In this study, Hg concentration in muscle, liver and kidney tissues and Se concentration in liver tissue from three predatory fish species: Macrodon ancylodon (Pescada), Menticirrhus americanus (Perna de Moca) and Micropogonias furnieri (Corvina) and two planctivorous species: Mugil liza (Tainha) and Sardella braziliensis (Sardinha) were determined. Mercury determination was performed using Cold Vapour Atomic Absorption Spectrometry (CV AAS) and selenium by Instrumental Neutron Activation Analysis (INAA). The muscle-Hg concentration variation (wet weight) was: (8 to 40 mug kg{sup -1}) - Sardinha; (12 to 62 mug kg{sup -1}) - Pescada; (3 to 23 mug kg{sup -1}) - Tainha; (43 to 184 mug kg{sup -1}) - Perna de Moca and (41 to 348 mug kg{sup -1}) - Corvina. The general concentration ranges of the analyzed elements in all species studied were (dry weight): muscle-Hg (13 to 1512 mug kg{sup -1}); liver-Hg (21 to 1804 mug kg{sup -1}); kidney-Hg 47 to 9912 mug kg{sup -1}) and liver-Se (2.10 to 43.00 mg kg{sup -1}). Se concentrations were higher than those of Hg in the liver. (author)

  2. Mercury and selenium accumulation assessment in fish most consumed by Cubatao Community, Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Farias, Luciana A.; Favaro, Deborah I.T.; Curcho, Michel R.S.M.; Fonseca, Barbara C.; Nascimento, Soraia M.

    2009-01-01

    High correlation between mercury (Hg) and selenium (Se) concentrations in fish organs is well known. In the present study, 58 fish samples of five fish species most commonly consumed by the Cubatao city population, Sao Paulo State, Brazil were analyzed. The Cubatao Estuary, located in southeastern Sao Paulo State, is an economically important area suffering severe environmental water quality problems due to industrial wastes, domestic sewage and solid residues. The waters of the bay suffer the impact of the immense industrial complex of Cubatao. In this study, Hg concentration in muscle, liver and kidney tissues and Se concentration in liver tissue from three predatory fish species: Macrodon ancylodon (Pescada), Menticirrhus americanus (Perna de Moca) and Micropogonias furnieri (Corvina) and two planctivorous species: Mugil liza (Tainha) and Sardella braziliensis (Sardinha) were determined. Mercury determination was performed using Cold Vapour Atomic Absorption Spectrometry (CV AAS) and selenium by Instrumental Neutron Activation Analysis (INAA). The muscle-Hg concentration variation (wet weight) was: (8 to 40 μg kg -1 ) - Sardinha; (12 to 62 μg kg -1 ) - Pescada; (3 to 23 μg kg -1 ) - Tainha; (43 to 184 μg kg -1 ) - Perna de Moca and (41 to 348 μg kg -1 ) - Corvina. The general concentration ranges of the analyzed elements in all species studied were (dry weight): muscle-Hg (13 to 1512 μg kg -1 ); liver-Hg (21 to 1804 μg kg -1 ); kidney-Hg 47 to 9912 μg kg -1 ) and liver-Se (2.10 to 43.00 mg kg -1 ). Se concentrations were higher than those of Hg in the liver. (author)

  3. Be-7 measured at ground air level and rainfall in the city of Sao Paulo

    International Nuclear Information System (INIS)

    Damatto, Sandra R.; Souza, Joseilton M.; Frujuele, Jonatan V.; Maduar, Marcelo F.; Leonardo, Lucio; Pecequilo, Brigitte R.S.

    2013-01-01

    The cosmogenic radionuclide 7 Be (T 1/2 = 53.3 d), produced in the upper atmosphere by cosmic ray spallation of oxygen and nitrogen, is one of the cosmogenic radionuclides that can be used as tracer for heavy metals and pollutants in the environment, tracer of soil erosion and sedimentation in lakes, among other examples. Their subsequent deposition to the land surface occurs as both wet and dry fallout, although it has been demonstrated that 7 Be fallout is primarily associated with precipitation. There is limited data on the concentration of 7 Be in rainfall and in particulate in the Southern Hemisphere and in Brazil, compared with data from the Northern Hemisphere. This paper presents the results obtained of 7 Be concentrations measured from April 2011 to June 2013, in samples of air at ground level, each fifteen days, and rainfall in all the rainy events that occurred at Instituto de Pesquisas Energeticas e Nucleares (IPEN), which has its campus located in the city of Sao Paulo, state of Sao Paulo, Brazil. The concentrations of 7 Be were measured by non-destructive gamma-ray spectrometry using an extended range closed-end coaxial Be-layer HPGe detector with 25% relative efficiency and associated electronic devices and live counting time varying from 100,000 s to 300,000 s. The results obtained of 7 Be in particulate and in rainfall were correlated to seasons, precipitation, temperature and sunspot number. The higher values obtained for the concentrations were in spring and summer time presenting good correlations with the amount of precipitation and sunspot number and a clear seasonal variation. (author)

  4. Computerized method for evaluating diagnostic image quality of calcified plaque images in cardiac CT: Validation on a physical dynamic cardiac phantom

    Energy Technology Data Exchange (ETDEWEB)

    King, Martin; Rodgers, Zachary; Giger, Maryellen L.; Bardo, Dianna M. E.; Patel, Amit R. [Department of Radiology, Committee on Medical Physics, University of Chicago, 5841 South Maryland Avenue, MC 2026, Chicago, Illinois 60637 (United States); Department of Diagnostic Radiology, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239 (United States); Department of Medicine, University of Chicago, 5841 South Maryland Avenue, MC 5084, Chicago, Illinois 60637 (United States)

    2010-11-15

    Purpose: In cardiac computed tomography (CT), important clinical indices, such as the coronary calcium score and the percentage of coronary artery stenosis, are often adversely affected by motion artifacts. As a result, the expert observer must decide whether or not to use these indices during image interpretation. Computerized methods potentially can be used to assist in these decisions. In a previous study, an artificial neural network (ANN) regression model provided assessability (image quality) indices of calcified plaque images from the software NCAT phantom that were highly agreeable with those provided by expert observers. The method predicted assessability indices based on computer-extracted features of the plaque. In the current study, the ANN-predicted assessability indices were used to identify calcified plaque images with diagnostic calcium scores (based on mass) from a physical dynamic cardiac phantom. The basic assumption was that better quality images were associated with more accurate calcium scores. Methods: A 64-channel CT scanner was used to obtain 500 calcified plaque images from a physical dynamic cardiac phantom at different heart rates, cardiac phases, and plaque locations. Two expert observers independently provided separate sets of assessability indices for each of these images. Separate sets of ANN-predicted assessability indices tailored to each observer were then generated within the framework of a bootstrap resampling scheme. For each resampling iteration, the absolute calcium score error between the calcium scores of the motion-contaminated plaque image and its corresponding stationary image served as the ground truth in terms of indicating images with diagnostic calcium scores. The performances of the ANN-predicted and observer-assigned indices in identifying images with diagnostic calcium scores were then evaluated using ROC analysis. Results: Assessability indices provided by the first observer and the corresponding ANN performed

  5. Hydrostatic pressure cells development for X-ray and neutrons experiments

    International Nuclear Information System (INIS)

    Passamai Junior, Jose Luis; Pinheiro, Christiano J.G.; Orlando, Marcos Tadeu D.; Passos, Carlos A.C.; Rossi, Jesualdo L.; Mazzocchi, Vera L.; Parente, Carlos B.R.; Mestnik Filho, Jose; Martinez, Luis G.; Melo, Francisco C.L. de

    2011-01-01

    A set of hydrostatic pressure cells was specially developed in order to be applied in X-ray diffraction, X-ray absorption and neutron diffraction experiments. For the experiments where X-rays are used, the pressure cells are built in a CuBe alloy body with two B 4 C anvils in order to allow the low absorption of the radiation. The B 4 C anvils were specially prepared in CTA - Centro Tecnico Aeroespacial - Sao Jose dos Campos - Brazil, in order to present enhanced X-ray transparency and high hardness. One of the advantage of the CuBe-body cell with B 4 C anvil is that it can be also used under magnetic fields, for instance for measurements of AC magnetic susceptibility under high hydrostatic pressures. The X-ray cells work in transmission mode and present a 2 mm diameter hole for the beam path. The X-ray beam pass through the hole and outgoing to the detector positioned in front of the pressure cell. A second type of pressure cell was developed in order to be used in neutron elastic scattering experiments, especially in neutron diffraction experiments. The neutron cell pressure cell was constructed in Zirconium alloy reinforced with carbon fibers composite in order to improve the mechanical resistance of his cylindrical geometry. The B 4 C pressure cells are available to users of the techniques of X-ray diffraction and absorption in the Brazilian National Synchrotron Laboratory - LNLS, at Campinas City. The neutron pressure cell is available to users at the neutron powder diffraction facility installed at the Nuclear and Energy Research Institute - IPEN, Sao Paulo. In this work will be shown details and drawings of the two types of hydrostatic pressure cells. (author)

  6. Sustainable Campus Program: University of Sao Paulo and its contribution to the reduction of environmental and economic impacts; Programa Campus Sutentavel: a Universidade de Sao Paulo e sua contribuicao a reducao dos impactos ambientais e economicos

    Energy Technology Data Exchange (ETDEWEB)

    Vilela, M M; Grimoni, J A.B.; Burani, G F [Universidade de Sao Paulo (IEE/USP), SP (Brazil). Inst. de Eletrotecnica e Energia; Massola, A M.A.; Barbosa, E J.S.; Hamzo, S T; Guarnieri, M C; Prist, R; Sonnewend, J E.S. [Universidade de Sao Paulo (COSESP/USP), SP (Brazil). Coordenadoria do Campus da Capital

    2010-07-01

    This paper presents the actions and the results obtained by the action of the University of Sao Paulo (USP) in reduction of environmental impacts caused by its activities, the creation of standards and indicators to monitor the results, the training of personnel and development models of public administration that can be adopted by other universities and city, state and federal administration. (author)

  7. Nuclear technology in secondary school in the city of Sao Paulo

    International Nuclear Information System (INIS)

    Benites, Daniela B.; Gordon, Ana Maria P.L.

    2009-01-01

    Nowadays, much has been said about nuclear technology development in Brazil. The importance of introducing the nuclear energy in the Brazilian energetic matrix has been now recognized by the society. There is a highly accepted assertion about the role developed by the energy offer for the country development, although it is wellknown that this is a necessary condition, but not enough. Another major issue is the environment preservation, so that future generations may live in adequate climatic and environmental conditions. Industrial activities and mining are some of the issues debated in the world scenario. Therefore the option to introduce nuclear energy in the country energetic matrix is a decision that should be supported by its knowledge. Nevertheless, do the youth know what nuclear energy is? What this kind of technology applications and implications are? To answer these questions, this project aims to analyze to what extent secondary schools are aware of nuclear technology and its applications, to verify whether there are the minimal necessary conditions for their interpretation and comprehension of related phenomena, as well as to analyze whether these students are able to critically opine on this subject. The methodology consists in using, as a data collection tool, a questionnaire applied to the students. For this purpose, the research field is constituted of the third year secondary school students, from public state schools, in the city of Sao Paulo. The data survey was restricted to schools belonging to three levels of classification, in the National Examination of Secondary Schools 2007. The questions proposed in the questionnaire were elaborated in considering what students, according to their age, were supposed to know concerning nuclear technology having as reference the 'Leis de Diretrizes e Bases da Educacao Nacional' (National Education Guidance Law), the 'Parametros Curriculares Nacionais' (National Curricula Parameters) and basic nuclear

  8. Transcriptional Regulation of Frizzled-1 in Human Osteoblasts by Sp1.

    Directory of Open Access Journals (Sweden)

    Shibing Yu

    Full Text Available The wingless pathway has a powerful influence on bone metabolism and is a therapeutic target in skeletal disorders. Wingless signaling is mediated in part through the Frizzled (FZD receptor family. FZD transcriptional regulation is poorly understood. Herein we tested the hypothesis that Sp1 plays an important role in the transcriptional regulation of FZD1 expression in osteoblasts and osteoblast mineralization. To test this hypothesis, we conducted FZD1 promoter assays in Saos2 cells with and without Sp1 overexpression. We found that Sp1 significantly up-regulates FZD1 promoter activity in Saos2 cells. Chromatin immunoprecipitation (ChIP and electrophoretic mobility shift (EMSA assays identified a novel and functional Sp1 binding site at -44 to -40 from the translation start site in the FZD1 promoter. The Sp1-dependent activation of the FZD1 promoter was abolished by mithramycin A (MMA, an antibiotic affecting both Sp1 binding and Sp1 protein levels in Saos2 cells. Similarly, down-regulation of Sp1 in hFOB cells resulted in less FZD1 expression and lower alkaline phosphatase activity. Moreover, over-expression of Sp1 increased FZD1 expression and Saos2 cell mineralization while MMA decreased Sp1 and FZD1 expression and Saos2 cell mineralization. Knockdown of FZD1 prior to Sp1 overexpression partially abolished Sp1 stimulation of osteoblast differentiation markers. Taken together, our results suggest that Sp1 plays a role in human osteoblast differentiation and mineralization, which is at least partially mediated by Sp1-dependent transactivation of FZD1.

  9. Suitability of Nicotiana tabacum 'Bel W3' for biomonitoring ozone in Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Sant'Anna, Silvia M.R.; Esposito, Marisia P.; Domingos, Marisa; Souza, Silvia R.

    2008-01-01

    Nicotiana tabacum 'Bel W3' is a widely used sensitive bioindicator for ambient ozone, but it is rarely used in tropical countries. Our goal was to determine the suitability of this plant for biomonitoring ozone in the city of Sao Paulo by evaluating the relationships between leaf necroses and ozone under field conditions and measurements of chlorophyll a fluorescence and antioxidants in plants exposed to different concentrations of ozone in closed chambers. While a weak linear relationship between leaf injury and ozone concentrations (R 2 = 0.10) was determined in the field, a strong linear relationship was observed in the chamber experiments. Maximum leaf injury was observed in plants submitted to 40 ppb, which coincided with a significant decrease in fluorescence and total ascorbic acid. The relationship between leaf damage observed in the field and ozone was improved when the concentrations were limited to 40 ppb (R 2 = 0.28). - Nicotiana tabacum 'Bel W3' is suitable for indicating low ozone levels in Brazil

  10. Primary production measurements at three reservoirs in the state of Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Jureidini, P.; Chinez, S.J.; Agudo, E.G.

    1983-01-01

    Primary production measurements were carried out at three reservoirs in the state of Sao Paulo, Barra Bonita, Paiva Castro and Ponte nova using the 14 C technique. Meanwhile, several physical and chemical parameters of these water were also evaluated, in order to find out the limnological conditions of these reservoirs. Primary production rates ranged from 7,6mg C/m 3 d at Ponte Nova, to 247,2mg C/m 3 d at Barra Bonita. There seems to be god correlation between water quality data and primary production measurements. Regarding the results, it may be stated that the Barra Bonita reservoir has reached the eutrophic level, while the other two exibit mesotrophic levels. As a way of testing the water quality data collected was used in Churchill and Nicholas model, issuing results in agreement with those of the primary production measurements. (Author) [pt

  11. La llegada de la peste al Estado de Sao Paulo en 1899

    OpenAIRE

    Nascimento, Dilene Raimundo do

    2011-01-01

    En octubre de 1899, la peste bubónica llegó a Brasil, por el puerto de Santos. Ciudad paulista de intenso flujo portuario fue la puerta de entrada de la epidemia de peste que asoló varias ciudades brasileñas a comienzos del siglo XX. Esta epidemia fue propulsora de una acción conjunta de varios estados para combatirla y, más que eso, ante las dificultades de importación de suero preparado en Europa, llevó a la creación, en 1899, del Instituto Butantan en Sao Paulo, y, en 1900, del Instituto S...

  12. Mineralogical characterization of historical portuguese wall tiles of Sao Luis, Maranhao, Brazil

    International Nuclear Information System (INIS)

    Rivas Mercury, J.M.; Vasconcelos, N.S.L.S.; Cabral, A.A.; Pereira, D.J. Costa; Angelica, R.S.

    2010-01-01

    Portuguese wall tiles from centuries, XVII, XVIII and XIX, found in Sao Luis Maranhao has been studied by X-ray diffraction, in order to interpret the possible raw material and burning temperature. The mineral phases, Quartz, Wollastonite, Calcite and Gehlenite were identified in all samples. Based on the results it is possible to affirm that the main raw materials used to manufacture this materials was probably mixtures of kaolinite clay and calcite. Based on the mineralogical information it was also possible to state that burning temperature of this wall tiles was lower than 1000 deg C. (author)

  13. Long-term safety and performance of the orbital atherectomy system for treating calcified coronary artery lesions: 5-Year follow-up in the ORBIT I trial

    Energy Technology Data Exchange (ETDEWEB)

    Bhatt, Parloop; Parikh, Parth [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India); Patel, Apurva [Internal Medicine, Cleveland Clinic Foundation, Cleveland, OH (United States); Chag, Milan; Chandarana, Anish [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India); Parikh, Roosha [Internal Medicine, Cleveland Clinic Foundation, Cleveland, OH (United States); Parikh, Keyur, E-mail: keyur.parikh@cims.me [Care Institute of Medical Sciences (CIMS), Ahmedabad 380060, Gujarat (India)

    2015-06-15

    Background/Purpose: The ORBIT I trial, a first-in-man study, was conducted to evaluate the safety and performance of the orbital atherectomy system (OAS) in treating de novo calcified coronary lesions. Methods/Materials: Fifty patients were enrolled between May and July 2008 based on several criteria, and were treated with the OAS followed by stent placement. The safety and performance of the OAS were evaluated by procedural success, device success, and overall major adverse cardiovascular event (MACE) rates, including cardiac death, myocardial infarction (MI) and need for target lesion revascularization (TLR). Our institution enrolled and treated 33 of the 50 patients and continued follow-up for 5 years. Results: Average age was 54 years and 91% were males. Mean lesion length was 15.9 mm. Device success was 100%, and average number of orbital atherectomy devices (OAD) used per patient was 1.3. Stents were placed directly after OAS in 31/32 patients (96.9%). All stents (average stent per lesion 1.1) were successfully deployed with 0.3% residual stenosis. The overall cumulative MACE rate was 6.1% in-hospital, 9.1% at 30 days, 12.1% at 6 months, 15.2% at 2 years, 18.2% at 3 years and 21.2% at 5 years (4 total cardiac deaths). None of the patients had Q-wave MIs. Angiographic complications were observed in 5 patients. No flow/slow flow due to distal embolization was observed. Conclusions: The ORBIT I trial suggests that OAS treatment continues to offer a safe and effective method to change compliance of calcified coronary lesions to facilitate optimal stent placement in these difficult-to-treat patients.

  14. Long-term safety and performance of the orbital atherectomy system for treating calcified coronary artery lesions: 5-Year follow-up in the ORBIT I trial.

    Science.gov (United States)

    Bhatt, Parloop; Parikh, Parth; Patel, Apurva; Chag, Milan; Chandarana, Anish; Parikh, Roosha; Parikh, Keyur

    2015-06-01

    The ORBIT I trial, a first-in-man study, was conducted to evaluate the safety and performance of the orbital atherectomy system (OAS) in treating de novo calcified coronary lesions. Fifty patients were enrolled between May and July 2008 based on several criteria, and were treated with the OAS followed by stent placement. The safety and performance of the OAS were evaluated by procedural success, device success, and overall major adverse cardiovascular event (MACE) rates, including cardiac death, myocardial infarction (MI) and need for target lesion revascularization (TLR). Our institution enrolled and treated 33 of the 50 patients and continued follow-up for 5 years. Average age was 54 years and 91% were males. Mean lesion length was 15.9 mm. Device success was 100%, and average number of orbital atherectomy devices (OAD) used per patient was 1.3. Stents were placed directly after OAS in 31/32 patients (96.9%). All stents (average stent per lesion 1.1) were successfully deployed with 0.3% residual stenosis. The overall cumulative MACE rate was 6.1% in-hospital, 9.1% at 30 days, 12.1% at 6 months, 15.2% at 2 years, 18.2% at 3 years and 21.2% at 5 years (4 total cardiac deaths). None of the patients had Q-wave MIs. Angiographic complications were observed in 5 patients. No flow/slow flow due to distal embolization was observed. The ORBIT I trial suggests that OAS treatment continues to offer a safe and effective method to change compliance of calcified coronary lesions to facilitate optimal stent placement in these difficult-to-treat patients. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  15. Long-term safety and performance of the orbital atherectomy system for treating calcified coronary artery lesions: 5-Year follow-up in the ORBIT I trial

    International Nuclear Information System (INIS)

    Bhatt, Parloop; Parikh, Parth; Patel, Apurva; Chag, Milan; Chandarana, Anish; Parikh, Roosha; Parikh, Keyur

    2015-01-01

    Background/Purpose: The ORBIT I trial, a first-in-man study, was conducted to evaluate the safety and performance of the orbital atherectomy system (OAS) in treating de novo calcified coronary lesions. Methods/Materials: Fifty patients were enrolled between May and July 2008 based on several criteria, and were treated with the OAS followed by stent placement. The safety and performance of the OAS were evaluated by procedural success, device success, and overall major adverse cardiovascular event (MACE) rates, including cardiac death, myocardial infarction (MI) and need for target lesion revascularization (TLR). Our institution enrolled and treated 33 of the 50 patients and continued follow-up for 5 years. Results: Average age was 54 years and 91% were males. Mean lesion length was 15.9 mm. Device success was 100%, and average number of orbital atherectomy devices (OAD) used per patient was 1.3. Stents were placed directly after OAS in 31/32 patients (96.9%). All stents (average stent per lesion 1.1) were successfully deployed with 0.3% residual stenosis. The overall cumulative MACE rate was 6.1% in-hospital, 9.1% at 30 days, 12.1% at 6 months, 15.2% at 2 years, 18.2% at 3 years and 21.2% at 5 years (4 total cardiac deaths). None of the patients had Q-wave MIs. Angiographic complications were observed in 5 patients. No flow/slow flow due to distal embolization was observed. Conclusions: The ORBIT I trial suggests that OAS treatment continues to offer a safe and effective method to change compliance of calcified coronary lesions to facilitate optimal stent placement in these difficult-to-treat patients

  16. Acid rain: a case study at the Universidade de Sao Paulo campus, Sao Paulo State, Brazil; Chuva acida: estudo de caso no campus USP/SP

    Energy Technology Data Exchange (ETDEWEB)

    Galvao, Patricia

    1997-12-31

    The phenomena called acid rain is considered, by many researchers, one of the most serious environmental problem. This work has the aim of showing, in a theoretical and practical study, the problems caused by the atmospheric-pollutant emission, through natural or anthropogenic sources. In a period of 1 year (nov/94-nov/95), it was realized a practical work on rainwater, which consisted of collecting and, afterwards, analysing some physical and chemical parameters of this water, such as acidity, ionic concentrations, etc, with the purpose of characterizing the rainwater in Cidade Universitaria (SP, Brazil). After ending the practical part, it was possible to observe a 1,236.71 mm/y pluviosity, characterized by rainy summer and dry winter. The chemical-constituent-concentration analysis show us the predominance of SO{sup 2-}{sub 4} and Ca{sup 2+}, and a continental-origin water). The region of sao Paulo (Brazil), site of this study, is one of the largest metropolitan and industrialized areas of the world, which includes 18 million people, beside to an enormous industrial and vehicular complex. The acidity in the rain water is a complex problem and it must be treated by a range of disciplines to have a better comprehension of the cause/effects of the acid rain. (author) 96 refs., 28 figs., 4 tabs.

  17. Acid rain: a case study at the Universidade de Sao Paulo campus, Sao Paulo State, Brazil; Chuva acida: estudo de caso no campus USP/SP

    Energy Technology Data Exchange (ETDEWEB)

    Galvao, Patricia

    1996-12-31

    The phenomena called acid rain is considered, by many researchers, one of the most serious environmental problem. This work has the aim of showing, in a theoretical and practical study, the problems caused by the atmospheric-pollutant emission, through natural or anthropogenic sources. In a period of 1 year (nov/94-nov/95), it was realized a practical work on rainwater, which consisted of collecting and, afterwards, analysing some physical and chemical parameters of this water, such as acidity, ionic concentrations, etc, with the purpose of characterizing the rainwater in Cidade Universitaria (SP, Brazil). After ending the practical part, it was possible to observe a 1,236.71 mm/y pluviosity, characterized by rainy summer and dry winter. The chemical-constituent-concentration analysis show us the predominance of SO{sup 2-}{sub 4} and Ca{sup 2+}, and a continental-origin water). The region of sao Paulo (Brazil), site of this study, is one of the largest metropolitan and industrialized areas of the world, which includes 18 million people, beside to an enormous industrial and vehicular complex. The acidity in the rain water is a complex problem and it must be treated by a range of disciplines to have a better comprehension of the cause/effects of the acid rain. (author) 96 refs., 28 figs., 4 tabs.

  18. Phurcalite and others secondary uranium minerals from Perus, Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Atencio, D.

    1991-01-01

    Phurcalite has been found filling fractures in the tourmaline-bearing granitic pegmatite of Perus, in the north-west part of Sao Paulo city, Brazil. It forms aggregates of radiating euhedral crystals up to 5 mm in length. The crystals are bright yellow, transparent and display vitreous to adamantine lustre. Its streak is pale yellow. Phurcalite is brittle, with a conchoidal fracture, and non-fluorescent. The crystal structure of phurcalite has been solves by single-crystal x-ray diffraction methods and refined to R = 3.8% using 2065 observed [I > 3σ(I)] reflections. The structure consists of [(U O 2 ) 3 O 2 (P O 4 ) 2 4n- ] n layers, parallel to (010), connected by Ca 2+ ions and H 2 O. The coordination polyhedra are: for U(1) hexagonal bi pyramid; for U(2) and U(3) pentagonal bi pyramids; for Ca(4) and Ca(5) capped trigonal prism and triangulated dodecahedron, respectively; and for P(6) and P(7) tetrahedra. As a consequence of this work, the molecular formula of phurcalite previously reported as Ca 2 (U O 2 ) 3 (P O 4 ) 2 (OH) 4 .4 H 2 O must be changed to Ca 2 (U O 2 ) 3 O 2 (P O 4 ) 2 .7 H 2 O. Other secondary uranium minerals associated with Perus phurcalite are autunite, torbernite, meta-autunite, meta-torbernite, chernikovite, meta-uranocircite I, phosphuranylite, uranophane-alpha, uranophane-beta, haiweeite, barian week site and perhaps also bassetite, meta-tyuyamunite and meta-haiweeite. Opal, tridymite, cristobalite, secondary quartz, saponite and rhodochrosite occur associated to the uranium minerals. (author)

  19. Challenges in modern biomarker discovery--17th HUPO BPP workshop: May 24-25, 2012, Sao Paulo, Brazil.

    Science.gov (United States)

    Gröttrup, Bernd; Esselmann, Hermann; May, Caroline; Schrötter, Andreas; Woitalla, Dirk; Heinsen, Helmut; Marcus, Katrin; Wiltfang, Jens; Meyer, Helmut E; Grinberg, Lea T; Park, Young Mok

    2013-01-01

    The HUPO Brain Proteome Project (HUPO BPP) held its 17(th) workshop in Sao Paulo, Brazil, on May 24 and 25, 2012. The focus was on the progress on the Human Brain Proteome Atlas as well as ideas, strategies and methodological aspects in clinical neuroproteomics. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Rocks age and metamorphic occurrence from the southeastern part of Sao Paulo State and their crustal evaluation

    International Nuclear Information System (INIS)

    Tassinari, C.C.G.

    1988-01-01

    Pb-Pb and Rb-Sr whole rock isotope systematics and U-Pb on zircons method analyses are reported for rocks from the southeastern part of Sao Paulo state Brazil. The isotopic studies on granitic intrusions, orthogneissic rocks and migmatitic terranes, in this area, provides an important indication of the age and nature of the continental crust. (author) [pt