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Sample records for salsola tragus prickly

  1. Identification of glyphosate resistance in Salsola tragus in north-eastern Oregon.

    Science.gov (United States)

    Barroso, Judit; Gourlie, Jennifer A; Lutcher, Larry K; Liu, Mingyang; Mallory-Smith, Carol A

    2018-05-01

    Farmers in the low-rainfall region of eastern Oregon rely on repeated applications of non-selective herbicides, predominately glyphosate, to control Salsola tragus in no-till fallow systems. Reports of poor glyphosate effectiveness have increased in recent years. Reduced efficacy is often attributed to dust, water stress, or generally poor growing conditions during application. Inadequate control also may be the result of the evolution of glyphosate resistance. Therefore, studies were undertaken to determine if glyphosate-resistant S. tragus populations occur in Oregon. Results from dose-response studies confirmed glyphosate resistance in three of 10 Oregon Salsola tragus populations. The ratio I 50R /I 50S from dose-response curves was, on average, 3.1 for the relative dry biomass per plant and 3.2 for the % of surviving plants per pot in these three populations. Plant mortality at recommended glyphosate doses for the resistant populations was less than 30% 3 weeks after treatment. Glyphosate resistance in S. tragus highlights the imperative need to diversify weed control strategies to preserve the longevity and sustainability of herbicides in semi-arid cropping systems of the Pacific Northwest. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  2. Briquetting soda weed (Salsola tragus) to be used as a rural fuel source

    Energy Technology Data Exchange (ETDEWEB)

    Yumak, Hasan [Mechanical Engineering Department, Faculty of Engineering and Architecture, Yuzuncu Yil University, 65080 Van (Turkey); Ucar, Tamer [Mechanical Engineering Department, Faculty of Engineering and Architecture, Bozok University, 66200 Yozgat (Turkey); Seyidbekiroglu, Nesim [Agricultural Machinery Department, Faculty of Agriculture, Yuzuncu Yil University, 65080 Van (Turkey)

    2010-05-15

    Amount of traditional fuel sources in the world has been decreasing and there is a definite need to produce and utilize alternative fuels such as biomass materials. In this study, briquetting conditions of Russian tumbleweed, Salsola tragus, (commonly named soda weed in Turkey) which grows in salty soils were investigated. Soda weeds were first chopped coarsely in a local tresher, then chopped finely in a hammer mill. Weed materials at three moisture levels (7%, 10%, and 13%) were prepared in the lab. Chopped weed materials were filled in cylindrical and square dies and compressed using a hydraulic press at three pressure levels of 15.7, 19.6 and 31.4 MPa. Optimum temperature, moisture rate, and pressure values were determined to produce stable briquettes. Further experiments were conducted to produce briquettes using sawdust and walnut shells as additives in conical dies of two different sizes. Results of a statistical analysis of parameters to produce briquettes in different dies indicated that moisture rates of 7-10%, pressure of 31.4 MPa, and temperatures of 85-105 C were suitable for briquetting soda weed. Furthermore, sawdust and walnut shells additives increased briquette density without any negative effects on production process and product stability. (author)

  3. Tolerance of the eriophyid mite Aceria salsolae to UV-A light and implications for biological control of Russian thistle.

    Science.gov (United States)

    Moran, Patrick J; Wibawa, M Irene; Smith, Lincoln

    2017-12-01

    Aceria salsolae (Acari: Eriophyidae) is being evaluated as a candidate biological control agent of Russian thistle (Salsola tragus, Chenopodiaceae), a major invasive weed of rangelands and dryland crops in the western USA. Prior laboratory host range testing under artificial lighting indicated reproduction on non-native Bassia hyssopifolia and on a native plant, Suaeda calceoliformis. However, in field tests in the native range, mite populations released on these 'nontarget' plants remained low. We hypothesized that UV-A light, which can affect behavior of tetranychid mites, would affect populations of the eriophyid A. salsolae differently on the target and nontarget plant species, decreasing the mite's realized host range. Plants were infested with A. salsolae under lamps that emitted UV-A, along with broad-spectrum lighting, and the size of mite populations and plant growth was compared to infested plants exposed only to broad-spectrum light. Russian thistle supported 3- to 55-fold larger mite populations than nontarget plants regardless of UV-A treatment. UV-A exposure did not affect mite populations on Russian thistle or S. calceoliformis, whereas it increased populations 7-fold on B. hyssopifolia. Main stems on nontarget plants grew 2- to 6-fold faster than did Russian thistle under either light treatment. The two nontarget plants attained greater volume under the control light regime than UV-A, but Russian thistle was unaffected. Although Russian thistle was always the superior host, addition of UV-A light to the artificial lighting regime did not reduce the ability of A. salsolae to reproduce on the two nontarget species, suggesting that UV-B or other environmental factors may be more important in limiting mite populations in the field.

  4. Systemic reaction after performing a food prick-to-prick test. A case report

    Directory of Open Access Journals (Sweden)

    Karen Estefanía Hernández-Moreno

    2017-02-01

    Full Text Available Background: Skin prick test is the most widely used test for the diagnosis of IgE-mediated conditions. Commercial extracts are used for its performance, but in the case of fruits and vegetables it is preferable using fresh food. Although both tests possess a good safety profile, hypersensitivity reactions have been recorded. Clinical case: Forty-seven-year old woman with a history of persistent allergic rhinitis, sensitized to the pollen of grasses, olive and salsola; she was referred to an allergology department due to anaphylaxis triggered by the consumption of avocado, cantaloupe, carrots and watermelon. Minutes after skin prick test with standardized extract and skin prick with fresh foods, she developed dyspnea, pruritus, erythema, dizziness and sibilance; she was administered 0.5 mg of intramuscular adrenalin and 4 salbutamol inhalations and placed in the Trendelemburg position. Dyspnea persisted, and vital signs monitoring showed heart and respiratory rates increase and, hence, salbutamol was applied again, together with 2 L/min of oxygen delivered by nasal cannula, intravenous fluids and 100 mg intravenous hydrocortisone; improvement was observed at 40 minutes. The patient was hospitalized for 48 hours. Conclusions: Although skin tests are safe, the risk of hypersensitivity and anaphylactic reactions should not be ruled out, especially in susceptible patients.

  5. Characterization of four salsola species and their genetic relationship by aflp

    International Nuclear Information System (INIS)

    Amal, M.E.; Hamid, A.

    2016-01-01

    Amplified length polymorphism (AFLP) technique was used to characterize and detect molecular genetic markers for four Salsola species collected from Al Jouf region in the northern of Saudi Arabia and to shed light on their genetic relationships. Three primer combinations were used for AFLP analysis of the four Salsola species, they generated a total of 181 fragments of which 133 were species specific markers scored across Salsola species. The dendogram produced by Jaccard's coefficient and the UPGMA clustering method showed one main cluster, subdivided into two subclusters. The first sub cluster included Salsola schweinfurthii and Salsola tetrandra. The second sub cluster included Salsola villosa and Salsola cyclophylla. It is worth mentioning that this is the first study to use AFLP markers to characterize and detect molecular genetic markers for the four Salsola species and their genetic relationships. (author)

  6. The role of tragus on echolocating bat, Eptesicus fuscus

    Science.gov (United States)

    Chiu, Chen; Moss, Cynthia

    2005-04-01

    Echolocating bats produce ultrasonic vocal signals and utilize the returning echoes to detect, localize and track prey, and also to avoid obstacles. The pinna and tragus, two major components of the bats external ears, play important roles in filtering returning echoes. The tragus is generally believed to play a role in vertical sound localization. The purpose of this study is to further examine how manipulation of the tragus affects a free-flying bat's prey capture and obstacle avoidance behavior. The first part of this study involved a prey capture experiment, and the bat was trained to catch the tethered mealworms in a large room. The second experiment involved obstacle avoidance, and the bat's task was to fly through the largest opening from a horizontal wire array without touching the wires. In both experiments, the bat performed the tasks under three different conditions: with intact tragus, tragus-deflection and recovery from tragus-deflection. Significantly lower performance was observed in both experiments when tragi were glued down. However, the bat adjusted quickly and returned to baseline performance a few days after the manipulation. The results suggest that tragus-deflection does have effects on both the prey capture and obstacle avoidance behavior. [Work supported by NSF.

  7. Resultados de miringoplastia Inlay com cartilagem de tragus Inlay tragus cartilage miringoplasty results

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    I.G.F. Couto

    2004-08-01

    Full Text Available A utilização de cartilagem de tragus para o fechamento de perfurações de membrana timpânica (MT com acesso transcanal foi primeiramente descrito por Eavey em 1998 com excelentes resultados quanto à pega do enxerto e vantagens como facilidade técnica, rapidez e conforto pós-operatório ao paciente. OBJETIVO: Avaliar os resultados da miringoplastia inlay com cartilagem de tragus em pacientes operados no serviço de Otorrinolaringologia do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo nos últimos três anos. FORMA DE ESTUDO: Clínico prospectivo. MATERIAL E MÉTODO: Foram utilizadas para esse estudo 32 cirurgias realizadas no serviço de Otorrinolaringologia do Hospital das Clínicas de Ribeirão Preto no período de 2000 a 2003 em pacientes com diagnóstico de otite média crônica simples com perfuração de membrana timpânica de até 5mm de diâmetro e sem contra-indicações para realização de procedimento transcanal. RESULTADOS: Foi observado fechamento completo das perfurações de MT em 28 cirurgias das 32 realizadas perfazendo 87,5% de sucesso. DISCUSSÃO: O presente estudo manteve o alto índice de sucesso no fechamento de perfurações de MT com os princípios da técnica de Eavey descrito em estudos anteriores, mesmo com algumas modificações em relação à técnica original. CONCLUSÃO: A miringoplastia inlay com cartilagem de tragus em forma de asa de borboleta tem alto índice de sucesso no fechamento das perfurações de MT de até 5mm de diâmetro em casos de otite média crônica simples com orelha média saudável. Promove, ainda, maior conforto e menor morbidade ao paciente.The use of tragus cartilage to close tympanic membrane (TM perforations with transcanal approach was first described by Eavey in 1998 with excellent results for graft "take-rate" and with advantages such as technique ease, rapidity and postoperative patient comfort. AIM: To evaluate the inlay

  8. A study of parallelism of the occlusal plane and ala-tragus line.

    Science.gov (United States)

    Sadr, Katayoun; Sadr, Makan

    2009-01-01

    Orientation of the occlusal plane is one of the most important clinical procedures in prostho-dontic rehabilitation of edentulous patients. The aim of this study was to define the best posterior reference point of ala-tragus line for orientation of occlusal plane for complete denture fabrication. Fifty-three dental students (27 females and 26 males) with complete natural dentition and Angel's Class I occlusal relationship were selected. The subjects were photographed in natural head position while clenching on a Fox plane. After tracing the photographs, the angles between the following lines were measured: the occlusal plane (Fox plane) and the superior border of ala-tragus, the occlusal plane (Fox plane) and the middle of ala-tragus as well as the occlusal plane (Fox plane) and the inferior border of ala-tragus. Descriptive statistics, one sample t-test and independent t-test were used. P value less than 0.05 was considered significant. There was no parallelism between the occlusal plane and ala-tragus line with three different posterior ends and one sample t-test showed that the angles between them were significantly different from zero (pplane. The superior border of the tragus is suggested as the posterior reference for ala-tragus line.

  9. Clinical Experiences in the Surgical Treatment of Accessory Tragus

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    Uğur Horoz

    2016-09-01

    Full Text Available Objective: Tragus is a part of the external ear that develops from the first branchial arch. Accessory ear is a congenital external ear anomaly and has skin elevation containing remnant cartilage. The auricle develops between the 4th and 12th week of the embryonic stage, which groove the tissue from the 1st and 2nd branchial arches. Histologically, the lesions include a rugated epidermis with a thin layer stratum corneum, tiny mature hair follicles, fat lobules, and connective tissue framework that may include a central cartilage core. The aim of this study was to evaluate the accessory tragus lesions with our clinical surgical treatment results. Material and Methods: Lesions usually located anterior to the tragus and along an imaginary line drawn from the tragus to the angle of the mouth. Twelve patients admitted to our clinic between October 2011 and November 2014 were included in this study. Results: Seven boys and five girls between two–13 years old underwent operation. In total, 28 accessory ears were excised. No complications were observed during the procedure, and no complaints were noted in the postoperative period. Conclusion: Generally, limited anomaly is associated with the first and second branchial arch anomalies. Surgical excision is the standard treatment for the lesions which usually due to the esthetic concerns.

  10. EFFECT OF Salsola vermiculata

    African Journals Online (AJOL)

    Algerian aride zone is rich with medicinal plant, Salsola vermiculata is one of the most traditional plant remedies in Bechar region. in this work, we tried to test the inhibitory effect on eight storage soft wheat fungi of various extracts of Salsola vermiculata in vitro and vivo by using grain storage by the coating method.

  11. Accessory tragus: a dentist's perspective

    OpenAIRE

    Khandelwal, Vishal; Banda, Naveen Reddy; Nayak, Ullal Anand; Banda, Vanaja Reddy

    2013-01-01

    Accessory tragus (AT) also referred as preauricular tag is a rudimentary tag of ear tissue This paper presents two specific cases: one hereditary and another sporadic case of AT. A general clinical description of AT, its associated syndromes, embryology aetiopathogenesis and management is discussed. A dentist can play an important role in spotting the AT during their head and neck examination. The presence of this defect can be correlated to other congenital defects of first branchial arch. O...

  12. Specificity of germination of heteromorphic seeds in four annuals (Salsola L.) at different temperatures in the Junggar basin

    International Nuclear Information System (INIS)

    Ning, L.; Feng, L.H.; Chi, L.Z.; Xia, C.Z.

    2015-01-01

    Salsola L. is a large genus of arid desert plants that are primarily distributed in the Junggar Basin, China. We analysed their ability to adapt to arid habitats by comparing differences in germination characteristics of the species and populations of Salsola affinis C. A. Mey, Salsola korshinskyi Drob., Salsola brachiata Pall. and Salsola nitraria Pall. We classified the 4 species into four types (A, B, C and D) according to seed wing and seed size, and the heteromorphic seeds were incubatedunder different temperature regimes (0/10 degree C, 5/15 degree C, 10/25 degree C and 20/35 degree C). The 4 species had the highest germination rates and germination potential at 0/1 C. Germination rates and potential decreased with increasing temperature. However, the change range of the germination rate among the four species was different. Type A and B seeds of S. affinis, S. nitraria and S. korshinskyi were dominant at all temperatures and decreased with increasing temperature. The germination rate of type C seeds was between that of type A, B and D seeds. D-type seeds had the lowest germination rate and the lowest germination potential under the four temperature regimes among the four species but the differences were not significant. The germination rates of the four types of S. brachiata seeds did not significantly change with temperature. These results suggest that Salsola spp. can germinate continuously from spring to autumn to adapt to moisture fluctuations in the desert. (author)

  13. Pollen morphology of some asiatic species of genus salsola (chenopodiaceae) and its taxonomic relationships

    International Nuclear Information System (INIS)

    Toderich, K.N.; Shuyskaya, E.V.; Ozturk, M

    2010-01-01

    Comparative studies on the pollen grain morphology of 27 Asiatic species of the genus Salsola were conducted by using scanning electron microscope (SEM analysis) in order to assess the taxonomic value of pollen traits. The pollen are radially symmetrical isopolar, pantopolyporate, spherical or subspheroid. The pollen characters like size, pore number, chord (C/D ratio), pore diameter, exine thickness, level of sinking of pore, convexness of mesoporial exine, spinule and minute-hole densities and number of spines on pore membrane appeared to be useful characters in distinguishing the species. Interesting intraspecific variations in pollen grain morphology were recorded for the C/D ratio. This parameter is highly specific, supporting the delimitation of Salsola species, and appears to be more conservative than some flower and fruit characters. The numerical value of form index comprising the ratio between the length of polar axis and diameter (P/E) also was an informative trait for delimitation of the species investigated here. Three pollen types were recognized. Euclidean distance was used to compute the dissimilarity matrix and a cladogram prepared. The quantitative characters of pollen morphology allowed clustering of groups and ordination analyses of species from different sections/subsections within genus Salsola. These features indicated that overall pollen traits reflect the current taxonomic boundaries, except for the Salsola species allocated to Climacoptera and Halothamnus, which should be accepted as separate genera. (author)

  14. Salsola sp. A of Flora Zambesiaca from the coast of Mozambique is Caroxylon littoralis (Amaranthaceae subfam. Salsoloideae), hitherto only known from Madagascar

    DEFF Research Database (Denmark)

    Friis, Ib; Holt, Sune

    2017-01-01

    We have identified plants from the coast of the Inhambane Province, Mozambique, with the incompletely known species Salsola sp. A of Flora Zambesiaca and with Caroxylon littoralis (Moq.) Akhani & Roalson (Salsola littoralis Moq.), a species hitherto believed to be endemic to Madagascar and Île...

  15. Anaphylaxis Secondary to Prick to Prick Tests to Foods and its Risk Factors

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    Lucy Vania Galindo-Pacheco

    2014-01-01

    Full Text Available The diagnosis of food allergy requires a proper anamnesis and diagnostic testing with skin prick tests with fresh foods and/or standardized allergen, or specific IgE tests. The risk of systemic reactions is of 15-23 per 100,000 skin tests performed by prick method, specically anaphylaxis at 0.02%. This paper reports the case of four patients, who while performing prick to prick test with fresh food presented anaphylactic reaction. Implicated foods were fruits of the Rosaceae, Anacardiaceae and Caricaceae families. The severity of anaphylaxis was: two patients with grade 4, one patient grade 2 and one grade 3, all with appropriate response to drug treatment. The risk factors identi ed were: female sex, personal history of atopy, previous systemic reaction to Hymenoptera venom, prior anaphylaxis to prick tests to aeroallergens. We found that a history of positive skin test for Betulla v, can be a risk factor for anaphylaxis in patients with oral syndrome. During testing prick to prick with food anaphylaxis can occur, so it should be made with aerial red team on hand. The history of positivity Betulla v is an additional risk factor in these patients.

  16. Relationship between the occlusal plane corresponding to the lateral borders of the tongue and ala-tragus line in edentulous patients.

    Science.gov (United States)

    Ghosn, Carole Abi; Zogheib, Carla; Makzoumé, Joseph E

    2012-09-01

    Definitions of the ala-tragus line (ATL) cause confusion, because the exact points of reference for this line do not agree. This study determined the relationship between the prosthetic occlusal plane (OP) corresponding to the lateral borders of the tongue and ATL which was established by using the inferior border of the ala of the nose and (1) the superior border of the tragus (ATL 1), (2) the tip (ATL 2) and (3) the inferior border of the tragus (ATL 3). Neutral zone moldings using phonation and autopolymerizing acrylic resin were recorded and leveled with the lateral borders of the tongue. Lateral cephalometric radiographs were taken of each subject by a standard method. Tracings were obtained on acetate paper to show the prosthetic OP and the three ATLs. The relationship between the prosthetic OP and each of ATL was measured for each subject. Mean and standard deviation values were then calculated for the relationship. Statistical analysis was performed using repeated measure analysis of variance followed by Bonferroni pairwise comparisons and Student's t-test (α = 0.05). Significant difference was found between the three mean angles (p = 0.001). There was no significant difference between the mean angle (5.00° ± 4.38) formed by OP and ATL 2, and the mean angle (4.90° ± 3.50) formed by OP and ATL 3 (p = 1.00) which revealed the smallest. The findings of this study indicated that ATLs, extending from the inferior border of the ala of the nose to (1) the tip of the tragus of the ear, and (2) the inferior border of the tragus presented the closest relationship to the prosthetic OP corresponding to the lateral borders of the tongue. When the ATL is used for orientation of the OP in denture construction, it would seem preferable to define it as running from the inferior border of the ala of the nose to the tip or to the inferior border of the tragus of the ear.

  17. Optical Sensing of Weed Infestations at Harvest.

    Science.gov (United States)

    Barroso, Judit; McCallum, John; Long, Dan

    2017-10-19

    Kochia ( Kochia scoparia L.), Russian thistle ( Salsola tragus L.), and prickly lettuce ( Lactuca serriola L.) are economically important weeds infesting dryland wheat ( Triticum aestivum L.) production systems in the western United States. Those weeds produce most of their seeds post-harvest. The objectives of this study were to determine the ability of an optical sensor, installed for on-the-go measurement of grain protein concentration, to detect the presence of green plant matter in flowing grain and assess the potential usefulness of this information for mapping weeds at harvest. Spectra of the grain stream were recorded continuously at a rate of 0.33 Hz during harvest of two spring wheat fields of 1.9 and 5.4 ha. All readings were georeferenced using a Global Positioning System (GPS) receiver with 1 m positional accuracy. Chlorophyll of green plant matter was detectable in the red (638-710 nm) waveband. Maps of the chlorophyll signal from both fields showed an overall agreement of 78.1% with reference maps, one constructed prior to harvest and the other at harvest time, both based on visual evaluations of the three green weed species conducted by experts. Information on weed distributions at harvest may be useful for controlling post-harvest using variable rate technology for herbicide applications.

  18. Short-term vegetation response following mechanical control of saltcedar (Tamarix spp.) on the Virgin River, Nevada, USA

    Science.gov (United States)

    Ostoja, Steven M.; Brooks, Matthew L.; Dudley, Tom; Lee, Steven R.

    2014-01-01

    Tamarisk (a.k.a. saltcedar, Tamarix spp.) is an invasive plant species that occurs throughout western riparian and wetland ecosystems. It is implicated in alterations of ecosystem structure and function and is the subject of many local control projects, including removal using heavy equipment. We evaluated short-term vegetation responses to mechanical Tamarix spp. removal at sites ranging from 2 to 5 yr post-treatment along the Virgin River in Nevada, USA. Treatments resulted in lower density and cover (but not eradication) of Tamarix spp., increased cover of the native shrub Pluchea sericia (arrow weed), decreased density and cover of all woody species combined, increased density of both native annual forbs and the nonnative annual Salsola tragus (prickly Russian-thistle), and lower density of nonnative annual grasses. The treated plots had lower mean woody species richness, but greater herbaceous species richness and diversity. Among herbaceous species, native taxa increased in richness whereas nonnative species increased in both species richness and diversity. Thus, efforts to remove Tamarix,/i> spp. at the Virgin River reduced vegetative cover contributing to fuel loads and probability of fire, and resulted in positive effects for native plant diversity, with mixed effects on other nonnative species. However, absolute abundances of native species

  19. A determination of occlusal plane comparing different levels of the tragus to form ala-tragal line or Camper's line: A photographic study.

    Science.gov (United States)

    Kumar, Sandeep; Garg, Sandeep; Gupta, Seema

    2013-02-01

    The purpose of this study was to determine accurately the part of the tragus to be used to form the Ala-Tragal line or Camper's line in orthognathic profile patients. 150 dentate subjects with age of 18-40 years with orthognathic profile were sampled. Life-size lateral digital photographs of the face with fox plane were taken in natural head position. Different angles between Eye-Ear plane and occlusal plane (OT1-OP), Eye-Ear plane and ala-superior border of tragus (OT1-AT1), Eye-Ear plane and ala-middle border of tragus (OT1-AT2) and Eye-Ear plane and ala-inferior border of tragus (OT1-AT3) were calculated using computer software package, AutoCAD 2004. From the three angles formed by the Eye-ear plane (OT1 or FH plane) and the ala-tragal lines, the one closest to the angle formed between Eye-Ear plane (OT1) and occlusal plane (OP) was used to determine the occlusal plane of orientation. The obtained results were subjected to ANOVA F test, Tukey's Honestly significant difference test, followed by Karl Pearson coefficient of correlation test. P values of less than 0.05 were taken as statistically significant. The mean of base line angle i.e. OT1-OP angle (11.96 ± 4.36) was found to be close to OT1-AT2 angle (13.67 ± 1.93) and OT1-AT3 angle (10.31 ± 2.03), but OT1-OP angle was found to be more closer to OT1-AT3 angle. Comparison of mean angles showed that OT1-OP angle in both males (11.68) and females (12.51) is close to OT1-AT3 angle (males- 11.01, females- 11.95). The line joining from ala to the lower border of the tragus was parallel to the occlusal plane in 53.3% of the subjects. There was no influence of the sex on the level of occlusal plane.

  20. Where to prick the apple for skin testing?

    NARCIS (Netherlands)

    Vlieg-Boerstra, B. J.; van de Weg, W. E.; van der Heide, S.; Dubois, A. E. J.

    Mal d 1 is not equally distributed over the apple. We aimed to examine the influence of the location of pricking in the apple on prick-to-prick skin prick test (PTP) results. PTPs were performed in autumn 2007 and spring 2008, before the birch pollen season, in 32 Dutch adults with symptoms of oral

  1. Where to prick the apple for skin testing?

    NARCIS (Netherlands)

    Vlieg-Boerstra, B.J.; Weg, van de W.E.; Heide, van der S.; Dubois, A.E.J.

    2013-01-01

    Mal d 1 is not equally distributed over the apple. We aimed to examine the influence of the location of pricking in the apple on prick-to-prick skin prick test (PTP) results. PTPs were performed in autumn 2007 and spring 2008, before the birch pollen season, in 32 Dutch adults with symptoms of oral

  2. Effects of temperature, relative humidity and moisture content on seed longevity of shrubby Russian thistle (Salsola vermiculata L.)

    NARCIS (Netherlands)

    Niane, A.A.; Struik, P.C.; Bishaw, Z.

    2013-01-01

    Salsola vermiculata is a highly palatable shrub and widely used in rangeland rehabilitation programs, but has short seed longevity. To identify the most cost effective storage method for S. vermiculata, experiments were carried out to test the effects of fruit bracts (wings), temperature regimes,

  3. Where to prick the apple for skin testing?

    Science.gov (United States)

    Vlieg-Boerstra, B J; van de Weg, W E; van der Heide, S; Dubois, A E J

    2013-09-01

    Mal d 1 is not equally distributed over the apple. We aimed to examine the influence of the location of pricking in the apple on prick-to-prick skin prick test (PTP) results. PTPs were performed in autumn 2007 and spring 2008, before the birch pollen season, in 32 Dutch adults with symptoms of oral allergy to fresh apple, using apples harvested in autumn 2007. PTPs with fresh intact and unpeeled Pink Lady, Golden Delicious, Elise, Santana and Modi apples were performed using material obtained from approximately 2 cm near the stalk (top), and the middle region. All PTP responses were greater when performed with apple material near the stalk than from the middle region. In 2007, these differences were statistically significant for Pink Lady, Golden Delicious and Elise, and in 2008, for Pink Lady and Modi. When performing PTPs, the apple should be pricked near the stalk rather than in the middle. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. Isoenzymatic and cytological studies of some Asiatic species of the genus Salsola

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    Aleksandra Wojnicka-Półtorak

    2014-01-01

    Full Text Available The genetic and cytological variability of population of three Salsola species from Asia was investigated, using isozyme electrophoresis and haematoxylin staining. Eight enzyme systems, representing 14-17 loci, were examined: 6PGD, DIA, G6PD, GDH, GOT, MDH, PGM and PGI. Analysis of the chromosome number revealed that the three species have the same number of chromosomes: 2n=18. Parameters describing genetic diversity indicate a very low level of genetic variation of the studied populations. The isozyme data support hypothesis that strong directional selection can result in lower level of genetic variation of arid plant populations.

  5. A recombinant Sal k 1 isoform as an alternative to the polymorphic allergen from Salsola kali pollen for allergy diagnosis.

    Science.gov (United States)

    Mas, Salvador; Boissy, Patrice; Monsalve, Rafael I; Cuesta-Herranz, Javier; Díaz-Perales, Araceli; Fernández, Javier; Colás, Carlos; Rodríguez, Rosalía; Barderas, Rodrigo; Villalba, Mayte

    2015-01-01

    The incidence of Amaranthaceae pollen allergy has increased due to the desertification occurring in many countries. In some regions of Spain, Salsola kali is the main cause of pollinosis, at almost the same level as olive and grass pollen. Sal k 1 - the sensitization marker of S. kali pollinosis - is used in clinical diagnosis, but is purified at a low yield from pollen. We aimed to produce a recombinant (r)Sal k 1 able to span the structural and immunological properties of the natural isoforms from pollen, and validate its potential use for diagnosis. Specific cDNA was amplified by PCR, cloned into the pET41b vector and used to transform BL21 (DE3) Escherichia coli cells. Immunoblotting, ELISA, basophil activation and skin-prick tests were used to validate the recombinant protein against Sal k 1 isolated from pollen. Sera and blood cells from S. kali pollen-sensitized patients and specific monoclonal and polyclonal antisera were used. rSal k 1 was produced in bacteria with a yield of 7.5 mg/l of cell culture. The protein was purified to homogeneity and structural and immunologically validated against the natural form. rSal k 1 exhibited a higher IgE cross-reactivity with plant-derived food extracts such as peanut, almond or tomato than with pollen sources such as Platanus acerifolia and Oleaceae members. rSal k 1 expressed in bacteria retains intact structural and immunological properties in comparison to the pollen-derived allergen. It spans the immunological properties of most of the isoforms found in pollen, and it might substitute natural Sal k 1 in clinical diagnosis. © 2015 S. Karger AG, Basel.

  6. Foot Skin Ischemic Necrosis following Heel Prick in a Newborn

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    Esad Koklu

    2013-01-01

    Full Text Available There are only a few reports on side effects after heel prick in neonates although heel prick has been performed all over the world for many years. The medicine staff had obtained only a drop of blood by pricking the baby’s heel using a lancet without compressing the heel or foot to measure his blood glucose level 3 hours after birth. However he developed a severe and hemorrhagic skin reaction on his entire left foot, beginning 30 minutes after obtaining the drop of blood by pricking the baby’s heel using a lancet. The lesion, which was treated with topical mupirocin and povidone-iodine solution daily, slowly decreased in size and had almost fully resolved within 3 weeks. He was healthy and 9 months old at the time of writing this paper. We herein report a case of foot skin ischemic necrosis following heel prick in a newborn. To our knowledge this patient is the first case of foot skin ischemic necrosis due to heel prick in newborns.

  7. Prick by Prick Induced Anaphylaxis in a Patient with Peanuts and Lupine Allergy: Awareness of Risks and Role of Component Resolved Diagnosis

    Science.gov (United States)

    Ciccarelli, Anna; Calabrò, Claudia; Imperatore, Clara; Scala, Guglielmo

    2014-01-01

    A case of anaphylaxis is reported in the course of a prick by prick with Lupinus albus and roasted peanut in a 20-year-old woman. We focused on some main topics. First of all it seems important to underscore the potential risks connected to the practice of the prick-by-prick with fresh foods in allergic patients, especially when testing cross-reactive substances, such as White Lupine, peanuts, or soy. It is important that clinicians who perform prick tests be aware of the risk related with in vivo tests in allergic patients. Second, we discuss the problem of the hidden allergens, such as White Lupine flour, or soy flour which are utilized to improve wheat flour because of their lower cost. Patients with a demonstrated allergy to peanuts should be assessed for lupine allergy and informed about the “hidden allergens” issue. Finally, we believe that component resolved diagnosis, the serum specific IgE against molecular components, that is normally considered a second-level diagnostic step has an important role even as a first line approach at least in some selected cases. PMID:25477973

  8. Structural adaptation of Salsola soda L. (Chenopodiaceae from inland and maritime saline area

    Directory of Open Access Journals (Sweden)

    Milić Dubravka M.

    2013-01-01

    Full Text Available The microscopic analysis of leaf and stem in two populations of Salsola soda was carried out in order to examine mechanism of anatomical adaptations to environmental condition on saline habitats and to determine if there exists a morpho-anatomical differentiation between populations from maritime and inland saline area. Analysis included 26 quantitative characters of leaf and stem. The results showed that both populations exhibited halomorphic and xeromorphic adaptations, which refered to ecological plasticity and adaptations of plants to their habitats. Our research also showed that S. soda had quite a stable morphoanatomical structure, since only quantitative changes were recorded. [Projekat Ministarstva nauke Republike Srbije, br. 173002

  9. Prickly pear induces upregulation of liver LDL binding in familial heterozygous hypercholesterolemia

    International Nuclear Information System (INIS)

    Palumbo, B.; Palumbo, R.; Efthimiou, Y.; Stamatopoulos, J.; Sinzinger, H.; Oguogho, A.; Budinsky, A.; Sinzinger, H.

    2003-01-01

    The hypoglycemic effect of prickly pear is well known by native local Indian population since a long time. Beside the beneficial effects on lipid metabolism, oxidation injury and platelet function has been claimed in experimental animals. We recently found an upregulation of apo-B/E receptor. We therefore examined 10 patients with isolated heterozygous familial hypercholesterolemia (FH) being enrolled in a dietary run-in phase of 6 weeks after dietary counselling and a further 6 weeks of prickly pear addition. Uptake of autologous 123 I-radiolabeled LDL was determined at entry as well as after 6 weeks of daily prickly pear ingestion. We found a significant (p 176.4 mg/dl; p 123 I-LDL binding by prickly pear in FH-patients in vivo and indicate that prickly pear exerts a significant hypolipidemic action via receptor upregulation. (author)

  10. Effects of pricking, sun-drying and sieving on Ginger (Zingiber ...

    African Journals Online (AJOL)

    The effect of pricking, sun drying and sieving on the color retention of Nigerian yellow bark ginger were investigated. The exterior and interior surface of fresh, pricked and sun dried medium sized Nigerian ginger rhizomes (Tafin giwa) were analysed for colour variation. The colour values of the exterior surface of fresh ginger ...

  11. The skin prick test – European standards

    Directory of Open Access Journals (Sweden)

    Heinzerling Lucie

    2013-02-01

    Full Text Available Abstract Skin prick testing is an essential test procedure to confirm sensitization in IgE-mediated allergic disease in subjects with rhinoconjunctivitis, asthma, urticaria, anapylaxis, atopic eczema and food and drug allergy. This manuscript reviews the available evidence including Medline and Embase searches, abstracts of international allergy meetings and position papers from the world allergy literature. The recommended method of prick testing includes the appropriate use of specific allergen extracts, positive and negative controls, interpretation of the tests after 15 – 20 minutes of application, with a positive result defined as a wheal ≥3 mm diameter. A standard prick test panel for Europe for inhalants is proposed and includes hazel (Corylus avellana, alder (Alnus incana, birch (Betula alba, plane (Platanus vulgaris, cypress (Cupressus sempervirens, grass mix (Poa pratensis, Dactilis glomerata, Lolium perenne, Phleum pratense, Festuca pratensis, Helictotrichon pretense, Olive (Olea europaea, mugwort (Artemisia vulgaris, ragweed (Ambrosia artemisiifolia, Alternaria alternata (tenuis, Cladosporium herbarum, Aspergillus fumigatus, Parietaria, cat, dog, Dermatophagoides pteronyssinus, Dermatophagoides farinae, and cockroach (Blatella germanica. Standardization of the skin test procedures and standard panels for different geographic locations are encouraged worldwide to permit better comparisons for diagnostic, clinical and research purposes.

  12. Utilization of Prickly Pear Peels to Improve Quality of Pan Bread

    International Nuclear Information System (INIS)

    Anwar, M.M.; Sallam, E.M.

    2016-01-01

    This investigation aimed to study utilization of prickly pear peels to improve quality of pan bread. Prickly pear peels powder added to wheat flour 72 % at levels 1.0 and 2.0% to make pan beard. In this study, evaluation of nutrients and chemical constitutes and functional properties of prickly pear peels as well as the rheological properties of dough contained prickly pear peels at levels 1% and 2% has been conducted. Then evaluated organoleptic characteristics of pan bread made of it also determined staling rate. Results showed that prickly pear peels had higher content of 32.67 % fiber, 14.25 % pectin and 87.82 % ascorbic acid, and higher contents of antioxidant components. It consists of 441.11 mg/100 g, total phenols, 35.2 flavonoids mg/100 g and DPPH radical-scavenging 62.14%, also water holding capacity was 1.8 ml H 2 O / g , oil holding capacity 2.35 (ml oil/g) and foam stability 7.15%. The major phenolic compounds Oleuro 1264.407, pyrogallo 1149.68, Benzoic 982.37, 3-oH Tyrosol 588.53, Ellagic 413.26, Chorogenic 271.10, Protocatechuic acid 176.02, P-oH- Benzoic 112.78, Epicatechin105.99, Gallic acid 61.26 ppm. The results revealed that addition of prickly pear peels to wheat flour increased the nutrition values of pan bread made of it due to high contents of fiber, ascorbic acid and natural antioxidants, and also decreased staling which improves the quality of pan bread, as well as increases shelf-life of pan bread.

  13. Hydrothermal synthesis of lindgrenite with a hollow and prickly sphere-like architecture

    International Nuclear Information System (INIS)

    Xu Jiasheng; Xue Dongfeng

    2007-01-01

    Lindgrenite [Cu 3 (OH) 2 (MoO 4 ) 2 ] with a hollow and prickly sphere-like architecture has been synthesized via a simple and mild hydrothermal route in the absence of any external inorganic additives or organic structure-directing templates. The hierarchical lindgrenite particles are hollow and prickly spheres, which are comprised of numerous small crystal strips that are aligned perpendicularly to the spherical surface. Two factors are important for the formation of hollow and prickly architecture in the present process. One is the general phenomenon of Ostwald ripening in solution, which can be responsible for the hollow structure; the other is that lindgrenite crystals have a rhombic growth habit, which plays an important role in the formation of prickly surface. Furthermore, Cu 3 Mo 2 O 9 with the similar size and morphology can be easily obtained by a simple thermal treatment of the as-prepared lindgrenite in air atmosphere. - Graphical abstract: Lindgrenite [Cu 3 (OH) 2 (MoO 4 ) 2 ] with a hollow and prickly sphere-like architecture has been synthesized via a hydrothermal route. The hierarchical lindgrenite particles are hollow and prickly spheres, which are comprised of numerous crystal strips that are aligned perpendicularly to the spherical surface. Cu 3 Mo 2 O 9 with the similar size and morphology can be easily obtained by a thermal treatment of the as-prepared lindgrenite

  14. Identification of low allergenic apple cultivars using skin prick tests and oral food challenges

    NARCIS (Netherlands)

    Vlieg-Boerstra, B. J.; van de Weg, W. E.; van der Heide, S.; Kerkhof, M.; Arens, P.; Heijerman-Peppelman, G.; Dubois, A. E. J.

    2011-01-01

    As oral allergy syndrome (OAS) symptoms to apple are frequent, we aimed to identify low allergenic apple cultivars and to validate the prick-to-prick skin prick test (SPT) as a suitable screening method. Sixty-eight apple cultivars were tested by SPTs in 33 Dutch adults with OAS, before and during

  15. Identification of low allergenic apple cultivars using skin prick tests and oral food challenges

    NARCIS (Netherlands)

    Vlieg-Boerstra, B. J.; van de Weg, W. E.; van der Heide, S.; Kerkhof, M.; Arens, P.; Heijerman-Peppelman, G.; Dubois, A. E. J.

    P>Background: As oral allergy syndrome (OAS) symptoms to apple are frequent, we aimed to identify low allergenic apple cultivars and to validate the prick-to-prick skin prick test (SPT) as a suitable screening method. Methods: Sixty-eight apple cultivars were tested by SPTs in 33 Dutch adults with

  16. Empirical models in the description of prickly pear shoot (Nopal drying kinetics

    Directory of Open Access Journals (Sweden)

    Emmanuel M. Pereira

    Full Text Available ABSTRACT The objective of this study was to describe the technological process involved in the drying kinetics of fresh-cut prickly pear shoots through numerical and analytical solutions. Shoots of two different prickly pear species were used, ‘Gigante’ and ‘Miúda’. Drying was performed at different temperatures (50, 60, 70 and 80 °C and weighing procedures were made continuously. The experimental data were expressed as moisture ratio. The Page model showed the best fit to the drying kinetics of minimally processed ‘Gigante’ and ‘Miúda’ prickly pear shoots, with the best coefficients of determination and Chi-square. Peleg and Wang & Singh models can not be used to simulate the drying of ‘Gigante’ and ‘Miúda’ prickly pear shoots within the evaluated range of temperatures, showing an incoherent graphic pattern.

  17. Identification of low allergenic apple cultivars using skin prick tests and oral food challenge tests

    NARCIS (Netherlands)

    Vlieg-Boerstra, B.J.; Weg, van de W.E.; Heide, van der S.; Arens, P.F.P.; Heijerman-Peppelman, G.; Dubois, A.E.J.

    2011-01-01

    Background: As oral allergy syndrome (OAS) symptoms to apple are frequent, we aimed to identify low allergenic apple cultivars and to validate the prick-to-prick skin prick test (SPT) as a suitable screening method. Methods: Sixty-eight apple cultivars were tested by SPTs in 33 Dutch adults with

  18. [YANG's pricking-cupping therapy for knee osteoarthritis: a multi-center randomized controlled trial].

    Science.gov (United States)

    Wang, Bo; Liu, Xiru; Hu, Zhihai; Sun, Aijun; Ma, Yanwen; Chen Yingying; Zhang, Xuzhi; Liu, Meiling; Wang, Yi; Wang, Shuoshuo; Zhang, Yunjia; Li, Yijing; Shen, Weidong

    2016-02-01

    To evaluate the clinical efficacy of YANG's pricking-cupping therapy for knee osteoar thritis (KOA). Methods This was a multi-center randomized parallel controlled trial. One hundred and seventy one patients with KOA were randomly allocated to a pricking-cupping group (89 cases) and a conventional acu puncture group (82 cases). Neixiyan (EX-LE 4), Dubi (ST 35) and ashi points were selected in the two groups. Patients in the pricking-cupping group were treated with YANG's pricking-cupping therapy; the seven-star needles were used to perform pricking at acupoints, then cupping was used until slight bleeding was observed. Patients in the conventional acupuncture group were treated with semi-standardized filiform needle therapy. The treatment was given for 4 weeks (from a minimum of 5 times to a maximum of 10 times). The follow-up visit was 4 weeks. The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and the visual analogue scale (VAS) were adopted for the efficacy assessments. The pain score, stiffness score, physical function score and total score of WOMAC were all reduced after 4-week treatment and during follow-up visit in the two groups (all P0. 05), each score and total score of WOMAC in the pricking-cupping group were lower than those in the conventional acupuncture group after 4-week treatment and during follow-up visit (Pcupping group were lower than those in the conventional acupuncture group after 4-week treatment and during follow-up visit (P cupping and conventional acupuncture therapy can both significantly improve knee joint pain and function in patients with KOA, which are relatively safe. The pricking cupping therapy is superior to conventional acupuncture with the identical selection of acupoints.

  19. A Simple Method for the Preparation of ZnO Prickly Spheres

    Institute of Scientific and Technical Information of China (English)

    Da Zhi LI; Xin Yu SONG; Si Xiu SUN; Jin Xin GUO

    2004-01-01

    The synthesis of ZnO prickly spheres using precipitation followed by heating treatment was investigated. Zn(OH)2 precursor was prepared by precipitation process using Zn(CH3COO)2·2H2O in mixed 1-propanol-water solvent. Sodium dodecyl sulfate (SDS) as the anionic surfactant was added to control the morphology. The size and structure of ZnO prickly spheres were studied using XRD, TEM and SEM. The results showed that the morphologies and size of the spheres strongly depended on the volume ratio of 1-propanol /water and molar ratio of SDS/Zn2+. ZnO prickly spheres composed of nanorods could be obtained, when the volume ratio of 1-propanol/water = 2:3 and the molar ratio of Zn2+/SDS ≈ 450:1.

  20. Phenolic Profiling and Evaluation of Contraceptive Effect of the Ethanolic Extract of Salsola imbricata Forssk. in Male Albino Rats

    Directory of Open Access Journals (Sweden)

    Naglaa Gamil Shehab

    2014-01-01

    Full Text Available Reported researches dealing with either composition or bioactivity of Salsola imbricata are limited. This study was conducted aiming to investigate the phenolic composition of the plant and evaluate its efficacy as male contraceptive. Polyphenols, namely, phenolic acids and flavonoids, were qualitatively and quantitatively analysed by RP-HPLC in the hydrolysed methanol extract using two different wavelengths, 280 and 330 nm. The efficiency of different solvents in extracting the plant phenolics was assessed via spectrophotometric determination of the total phenolic and flavonoid contents. Acute toxicity study was carried out on the ethanolic extract to ascertain its safety prior to biological evaluation. The contraceptive effect was assessed, in male rats, by oral administration of the extract at two doses (250 and 500 mg/kg b. wt., over a period of 65 days. HPLC analyses allowed the identification and quantification of a total of 13 and 8 components in the hydrolysed-methanol extract; the overall phenolic composition was dominated by quercitrin (12.692% followed by coumaric acid (4.251%. Prolonged oral administration of the ethanolic extract caused slight reduction in the testis weight only. A significant decrease in the sperm count was observed (P<0.01 in the two treated groups while significant decrease in the epididymal sperm motility was only observed in the high dose group. Morphological abnormalities were observed in sperms of treated animals. No distinct change in serum FSH, LH, and testosterone concentration was recorded. The histopathological findings supported to a high extent these results. The male contraceptive activity of Salsola imbricata could be ascribed to its phenolic components, especially quercitrin.

  1. Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery

    Science.gov (United States)

    Chu, Zhiqin; Miu, Kaikei; Lung, Pingsai; Zhang, Silu; Zhao, Saisai; Chang, Huan-Cheng; Lin, Ge; Li, Quan

    2015-06-01

    The prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds’ escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds’ cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.

  2. Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery

    Science.gov (United States)

    Chu, Zhiqin; Miu, Kaikei; Lung, Pingsai; Zhang, Silu; Zhao, Saisai; Chang, Huan-Cheng; Lin, Ge; Li, Quan

    2015-01-01

    The prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds’ escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds’ cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier. PMID:26123532

  3. Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery.

    Science.gov (United States)

    Chu, Zhiqin; Miu, Kaikei; Lung, Pingsai; Zhang, Silu; Zhao, Saisai; Chang, Huan-Cheng; Lin, Ge; Li, Quan

    2015-06-30

    The prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds' escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds' cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.

  4. Evaluation Of Prick Test In Atopic Dermatitis And Chronic Urticaria

    Directory of Open Access Journals (Sweden)

    Dhar Sandipan

    1997-01-01

    Full Text Available “Prick test” was carried out in 15 patients with atopic dermatitis (AD and 10 patients with chronic urticaria (CU. Of the various aeroallergens tested, house dust mite (HDM, pollens, aspergillus furnigatus and insects were found to be most commonly positive. The common food allergens showing prick test positivity were egg white, fish, milk, brinjal, dal, groundnut and banana. Use of nasal filters showed 10-20% improvement in AD and 5 â€" 10% improvement in urticaria. Withdrawal of the responsible food article(s showed 20-30% improvement in patients with AD and urticaria.

  5. Extraction and determination of polyphenols and betalain pigments in the Moroccan Prickly pear fruits (Opuntia ficus indica

    Directory of Open Access Journals (Sweden)

    Omar Khatabi

    2016-09-01

    This study has permitted us equally to value betalain pigments extracted from fruity juice. These are the betalains present in the epidermis and the pulp of the prickly pear confers on it its color varying from yellow to purple. Results show that yellow and red prickly pears contain imported strengths in betalains. Our work shows that the red prickly pear contains betaxanthin pigments in excess of the indicaxanthin that permits to valorize human’s potential spring of genuine colorings. Betalains and polyphenols are antioxidants that contribute to nutritional prickly pears’ quality and to their products of transformation.

  6. Natural history of skin prick test reactivity

    DEFF Research Database (Denmark)

    Schou Nielsen, Jesper; Meteran, Howraman; Ulrik, Charlotte Suppli

    2017-01-01

    population sample of children and adolescents in relation to symptoms of rhinitis. METHODS: A total of 983 individuals, aged 7 to 17 years, were randomly selected in 1986 and invited to 4 examinations during a 20-year period. During each examination, a skin prick test was performed using common local...

  7. [Diagnostic capacity of skin prick test in egg and cow's milk allergic infants].

    Science.gov (United States)

    Yan, Jun-mei; Chen, Jing; Li, Hai-qi; Hu, Yan

    2011-05-01

    Mean diameter is the most common used parameter for wheal response assessment after skin prick test. This study aimed to investigate the diagnostic capacity of mean diameter according to the outcome of oral food challenge, and to determine the cut-off points that could render food challenges unnecessary. Data of 173 children referred to the Division of Primary Child Health Care for the evaluation of suspected food allergy were prospectively studied. All children underwent skin prick test and open food challenge to the relevant food(s) in clinic. The mean wheal diameter of skin prick test was measured, and open food challenge was performed to confirm food allergy. The SPSS software package version 13.0 for windows (SPSS, Chicago, IL, USA) was used for all statistical analysis. Open food challenge was taken as the gold standard for diagnosis. Diagnostic capacity of skin prick test, including the sensitivity, specificity, positive predictive value, negative predictive value, was calculated by cross-table. In addition, receiver operating characteristic curve (ROC) was plotted and area under the curve (AUC) was calculated to quantify the accuracy of the parameter. For the 173 children, 271 open food challenges were performed with egg white, egg yolk and cow's milk, In which 123 were positive, 99 children were diagnosed as food allergy. Cutaneous symptoms (87.0%) were most common, followed by gastrointestinal symptoms (9.8%). The AUC of mean diameter was 0.794 for egg white, 0.804 for egg yolk and 0.904 for cow's milk. The sensitivity of skin prick test with a cut-off value of ≥ 3 mm was ranged from 71% to 87%, while the specificity was between 31% and 57%. The authors also defined food specific skin prick test mean diameters that were 100% diagnostic for allergy to egg white (≥ 8.5 mm), egg yolk (≥ 5.5 mm), cow's milk (≥ 5.5 mm). Predictive decision points for a positive outcome of food challenges can be calculated for egg and cow's milk using mean diameter. It

  8. Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking.

    Science.gov (United States)

    Tan, Hong-Kee; Toh, Cheng-Xu Delon; Ma, Dongrui; Yang, Binxia; Liu, Tong Ming; Lu, Jun; Wong, Chee-Wai; Tan, Tze-Kai; Li, Hu; Syn, Christopher; Tan, Eng-Lee; Lim, Bing; Lim, Yoon-Pin; Cook, Stuart A; Loh, Yuin-Han

    2014-05-01

    Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a "do-it-yourself" basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide.

  9. Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice

    Directory of Open Access Journals (Sweden)

    Alejandra Hernández-Ceruelos

    2013-10-01

    Full Text Available Plants belonging to the genus Opuntia spp. are the most abundant of the Cactaceae family, grown throughout America and the Mediterranean central area. Its fruit, known as cactus pear or prickly pear, is an oval berry grouped in different colors. Some studies have shown its antioxidant activities which may help in preventing chronic pathologies such as diabetes. The purpose of the study was to evaluate the antioxidant capacity of three varieties of prickly pear juice (red-purple, white-green and yellow-orange in five different concentrations (100, 250, 500, 750, and 1000 mg/mL by DPPH (1,1-diphenyl-2-picrylhydrazyl radical colorimetric method, selecting the best variety to determine its anticlastogenic potential against methyl methanesulfonate (MMS. The results indicate that the highest antioxidant was found in the juice of the prickly pear red-purple variety (PPRP, in all concentrations. Its anticlastogenic potential was therefore evaluated with a micronucleus assay. The experiment was run over two weeks. A negative control was included along with a positive control with MMS (40 mg/kg, a group of mice treated with PPRP (25 mL/kg, and three groups with PPRP (in doses of 25, 16.5 and 8.3 mL/kg plus the mutagen. The PPRP was administered daily by oral gavage and the MMS was injected intraperitoneally five days prior to the end of the experiment. Blood samples were obtained at 0, 24, 48, 72 and 96 h in order to determine the frequency of micronucleated polychromatic erythrocytes (MNPE. The results indicated that PPRP is not a genotoxic agent, on the contrary, it may reduce the number of MNPE. In this regard, the PPRP showed an anticlastogenic effect directly proportional to its concentrations. Thus, the highest protection was obtained with a concentration of 25 mL/kg after 48 h of treatment.

  10. Effect of EDTA on Pb(II) Uptake and Translocation by Tumbleweed (Salsola Kali): Agar and Hydroponics Studies

    Energy Technology Data Exchange (ETDEWEB)

    de la Rosa, Guadalupe; Gardea-Torresdey, Jorge L.; Peralta-Videa, Jose R.; Aldrich, Mary

    2004-03-31

    Environmental accumulation of Pb represents a worldwide health hazard. While conventional cleanup techniques are generally expensive and soil disturbing, phytoremediation represents an inexpensive friendly option for the removal of contaminants from soil and water. In this research, tumbleweed (Salsola kali) plants exposed for 15 days to Pb(NO3)2 at 80 and 125 ppm in hydroponics and agar media, demonstrated a high capacity to uptake lead. The results showed that the plants cultivated in agar accumulated 25563, 5534 and 2185 mg Pb kg-1 DW in roots, stems and leaves, respectively. Moreover, Pb concentrations found in hydroponically grown tumbleweed plants tissues were 30744, 1511 and 1421 mg kg-1 DW in roots, stems and leaves, respectively. It was observed that EDTA enhanced Pb translocation. No Pb phytotoxic effects were observed during the experimental time period. Cellular structural features were also observed using TEM.

  11. [Effects of Chinese prickly ash orchard on soil organic carbon mineralization and labile organic carbon in karst rocky desertification region of Guizhou province].

    Science.gov (United States)

    Zhang, Wen-Juan; Liao, Hong-Kai; Long, Jian; Li, Juan; Liu, Ling-Fei

    2015-03-01

    Taking 5-year-old Chinese prickly ash orchard (PO-5), 17-year-old Chinese prickly ash orchard (PO- 17), 30-year-old Chinese prickly ash orchard (PO-30) and the forest land (FL, about 60 years) in typical demonstration area of desertification control test in southwestern Guizhou as our research objects, the aim of this study using a batch incubation experiment was to research the mineralization characteristics of soil organic carbon and changes of the labile soil organic carbon contents at different depths (0-15 cm, 15-30 cm, and 30-50 cm). The results showed that: the cumulative mineralization amounts of soil organic carbon were in the order of 30-year-old Chinese prickly ash orchard, the forest land, 5-year-old Chinese prickly ash orchard and 17-year-old Chinese prickly ash orchard at corresponding depth. Distribution ratios of CO2-C cumulative mineralization amount to SOC contents were higher in Chinese prickly ash orchards than in forest land at each depth. Cultivation of Chinese prickly ash in long-term enhanced the mineralization of soil organic carbon, and decreased the stability of soil organic carbon. Readily oxidized carbon and particulate organic carbon in forest land soils were significantly more than those in Chinese prickly ash orchards at each depth (P < 0.05). With the increasing times of cultivation of Chinese prickly ash, the contents of readily oxidized carbon and particulate organic carbon first increased and then declined at 0-15 cm and 15-30 cm depth, respectively, but an opposite trend was found at 30-50 cm depth. At 0-15 cm and 15-30 cm, cultivation of Chinese prickly ash could be good for improving the contents of labile soil organic carbon in short term, but it was not conducive in long-term. In this study, we found that cultivation of Chinese prickly ash was beneficial for the accumulation of labile organic carbon at the 30-50 cm depth.

  12. Histamine 50-skin-prick test: a tool to diagnose histamine intolerance.

    Science.gov (United States)

    Kofler, Lukas; Ulmer, Hanno; Kofler, Heinz

    2011-01-01

    Background. Histamine intolerance results from an imbalance between histamine intake and degradation. In healthy persons, dietary histamine can be sufficiently metabolized by amine oxidases, whereas persons with low amine oxidase activity are at risk of histamine toxicity. Diamine oxidase (DAO) is the key enzyme in degradation. Histamine elicits a wide range of effects. Histamine intolerance displays symptoms, such as rhinitis, headache, gastrointestinal symptoms, palpitations, urticaria and pruritus. Objective. Diagnosis of histamine intolerance until now is based on case history; neither a validated questionnaire nor a routine test is available. It was the aim of this trial to evaluate the usefullness of a prick-test for the diagnosis of histamine intolerance. Methods. Prick-testing with 1% histamine solution and wheal size-measurement to assess the relation between the wheal in prick-test, read after 20 to 50 minutes, as sign of slowed histamine degradation as well as history and symptoms of histamine intolerance. Results. Besides a pretest with 17 patients with HIT we investigated 156 persons (81 with HIT, 75 controls): 64 out of 81 with histamine intolerance(HIT), but only 14 out of 75 persons from the control-group presented with a histamine wheal ≥3 mm after 50 minutes (P < .0001). Conclusion and Clinical Relevance. Histamine-50 skin-prickt-test offers a simple tool with relevance.

  13. Analgesia with breastfeeding in addition to skin-to-skin contact during heel prick.

    Science.gov (United States)

    Marín Gabriel, Miguel Ángel; del Rey Hurtado de Mendoza, Beatriz; Jiménez Figueroa, Lourdes; Medina, Virginia; Iglesias Fernández, Beatriz; Vázquez Rodríguez, María; Escudero Huedo, Virginia; Medina Malagón, Lorena

    2013-11-01

    To investigate the analgesic effect (measured with Neonatal Infant Pain Scale (NIPS)) of breastfeeding (BF) in addition to skin-to-skin contact (SSC) versus other methods of non-pharmacological analgesia during blood sampling through heel lance in healthy term neonates. Randomised controlled trial. Tertiary level maternity ward. One hundred thirty-six healthy term newborns. healthy term neonates, wish to breastfeed and absence of feeding during the previous 60 min. Neonates were randomly assigned to four groups: Group breastfed with SSC (BF+SSC Group) (n=35); Group sucrose with SSC (Sucrose+SSC Group) (n=35); SSC Group (n=33); or Sucrose Group (n=33). Babies were recorded with a video camera. Three observers watched the videos and measured NIPS score at three time points (t0: 2 min before heel prick; t1: During heel prick; and t2: 2 min after the heel prick). The influences of non-pharmacological methods on crying time, percentage of crying while sampling, heart rate, number of attempts and duration of sampling were also studied. BF+SSC Group achieved a significant lower median NIPS score (value=1) compared with other groups (value=2, 4 and 4, respectively). The percentage of neonates with moderate-to-severe pain was also lower in the BF+SSC Group. Both groups BF+SSC and Sucrose+SSC achieved a significant lower percentage of crying compared with SSC Group. This study suggests that BF in addition to SSC provides superior analgesia to other kinds of non-pharmacological analgesia in healthy term neonates during heel prick.

  14. Trial of Music, Sucrose, and Combination Therapy for Pain Relief during Heel Prick Procedures in Neonates.

    Science.gov (United States)

    Shah, Swapnil R; Kadage, Shahajahan; Sinn, John

    2017-11-01

    To compare the effectiveness of music, oral sucrose, and combination therapy for pain relief in neonates undergoing a heel prick procedure. This randomized, controlled, blinded crossover clinical trial included stable neonates >32 weeks of postmenstrual age. Each neonate crossed over to all 3 interventions in random order during consecutive heel pricks. A video camera on mute mode recorded facial expressions, starting 2 minutes before until 7 minutes after the heel prick. The videos were later analyzed using the Premature Infant Pain Profile-Revised (PIPP-R) scale once per minute by 2 independent assessors, blinded to the intervention. The PIPP-R scores were compared between treatment groups using Friedman test. For the 35 participants, the postmenstrual age was 35 weeks (SD, 2.3) with an average weight of 2210 g (SD, 710). The overall median PIPP-R scores following heel prick over 6 minutes were 4 (IQR 0-6), 3 (IQR 0-6), and 1 (IQR 0-3) for the music, sucrose, and combination therapy interventions, respectively. The PIPP-R scores were significantly lower at all time points after combination therapy compared with the groups given music or sucrose alone. There was no difference in PIPP-R scores between the music and sucrose groups. In relatively stable and mature neonates, the combination of music therapy with sucrose provided better pain relief during heel prick than when sucrose or music was used alone. Recorded music in isolation had a similar effect to the current gold standard of oral sucrose. www.anzctr.org.au ACTRN12615000271505. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. Pattern Of Skin Prick Allergy Test Results In Enugu | Mgbor ...

    African Journals Online (AJOL)

    In this study we report on pattern of allergy prick skin test results found among atopic patients attending the department of otorhinolargngology of the University of Nigeria Teaching Hospital Enugu and Hansa Clinics, Enugu and propose ways of minimizing the exposure of the population to allergens. Material and method

  16. Chemical analysis of nutritional content of prickly pads (Opuntia ficus indica) at varied ages in an organic harvest.

    Science.gov (United States)

    Hernández-Urbiola, Margarita I; Pérez-Torrero, Esther; Rodríguez-García, Mario E

    2011-05-01

    Opuntia ficus indica, also known as prickly pads, are an important part of the human diet and are also used as forage for livestock. This is an interesting vegetable due the environmental conditions in which it grows and its resistance to climatic extremes; however, little is known about its nutritional properties, especially in the later stages of maturity. The objective of this study was to determine the composition of organic prickly pads (Opuntia ficus indica) at differing stages of growth maturity. Chemical proximate analysis and mineral constituent analysis at different maturation stages were carried out in this investigation. As a result, older prickly pads were found to be an important source of nutritional components such as calcium.

  17. Compatibility of Clinical Manifestation with Skin Prick Test Result and Food Provocation Test in Food Cross Reaction

    OpenAIRE

    Lubis, Azwin; Barlianto, Wisnu; Endaryanto, Anang; Harsono, Ariyanto

    2017-01-01

    Background: Subjective diagnostic test of food allergy is hugely biased, resulting in irrational diet avoidance. Additional objective tools by skin prick test following food provocation test resulting more accurate cause and prevalence on population. Purpose: To evaluate the compatibility of clinical symptoms with skin prick test and provocation test for imunoglobulin E (IgE) mediated food allergy in Dr. Soetomo Hospital Surabaya. Methods: Cross sectional observational analytic study. Patient...

  18. Low-Level Tragus Stimulation for the Treatment of Ischemia and Reperfusion Injury in Patients With ST-Segment Elevation Myocardial Infarction: A Proof-of-Concept Study.

    Science.gov (United States)

    Yu, Lilei; Huang, Bing; Po, Sunny S; Tan, Tuantuan; Wang, Menglong; Zhou, Liping; Meng, Guannan; Yuan, Shenxu; Zhou, Xiaoya; Li, Xuefei; Wang, Zhuo; Wang, Songyun; Jiang, Hong

    2017-08-14

    The aim of this study was to investigate whether low-level tragus stimulation (LL-TS) treatment could reduce myocardial ischemia-reperfusion injury in patients with ST-segment elevation myocardial infarction (STEMI). The authors' previous studies suggested that LL-TS could reduce the size of myocardial injury induced by ischemia. Patients who presented with STEMI within 12 h of symptom onset, treated with primary percutaneous coronary intervention, were randomized to the LL-TS group (n = 47) or the control group (with sham stimulation [n = 48]). LL-TS, 50% lower than the electric current that slowed the sinus rate, was delivered to the right tragus once the patients arrived in the catheterization room and lasted for 2 h after balloon dilatation (reperfusion). All patients were followed for 7 days. The occurrence of reperfusion-related arrhythmia, blood levels of creatine kinase-MB, myoglobin, N-terminal pro-B-type natriuretic peptide and inflammatory markers, and echocardiographic characteristics were evaluated. The incidence of reperfusion-related ventricular arrhythmia during the first 24 h was significantly attenuated by LL-TS. In addition, the area under the curve for creatine kinase-MB and myoglobin over 72 h was smaller in the LL-TS group than the control group. Furthermore, blood levels of inflammatory markers were decreased by LL-TS. Cardiac function, as demonstrated by the level of N-terminal pro-B-type natriuretic peptide, the left ventricular ejection fraction, and the wall motion index, was markedly improved by LL-TS. LL-TS reduces myocardial ischemia-reperfusion injury in patients with STEMI. This proof-of-concept study raises the possibility that this noninvasive strategy may be used to treat patients with STEMI undergoing primary percutaneous coronary intervention. Copyright © 2017. Published by Elsevier Inc.

  19. Rehabilitation of Degraded Rangeland in Drylands by Prickly Pear (Opuntia ficus-indica L. Plantations: Effect on Soil and Spontaneous Vegetation

    Directory of Open Access Journals (Sweden)

    Souad Neffar

    2013-12-01

    Full Text Available In arid and semi-arid lands, the spiny prickly pear (Opuntia ficus-indica is an outstanding plant for soil conservation and restoration. To determine the role of Opuntia ficus-indica on vegetation recovery process in desertified areas of Southern Tebessa (Northeast Algeria, we investigated the effect of prickly pear plantation age and some soil properties (grain size, pH, electrical conductivity, organic matter, total nitrogen, available phosphorus, and CaCO3 equivalents on native plant community. Vegetation cover and plant diversity were assessed by calculating the number of individual plants (N, species richness (S, their ratio (N/S, Shannon index, and Evenness in prickly pear plantation plots of different ages (control, 5 and 20 years. Even if surveyed soil parameters did not differ significantly among O. ficus-indica plantations, results of ANOVA testing the effect of Opuntia plantations on native vegetation traits revealed significant variation for plant abundance (P < 0.0001, N/S ratio (P = 0.003 and vegetation cover (P < 0.0001. Vegetation cover differed significantly with both prickly-pear plantation age (P = 0.031 and seasons (P = 0.019. Tukey's tests revealed that all vegetation traits were significantly higher on prickly pear plantations than in control plots. Multiple comparisons also showed that plant abundance, N/S ratio and vegetation cover were significantly different between both young and old plantations and the controls. Prickly pear cultures facilitated the colonization and development of herbaceous species by ameliorating the severe environmental conditions. In conclusion, the facilitative effect of O. ficus-indica has been clearly demonstrated for both abundance and cover of native vegetation.

  20. Ergonomic evaluation of conventional and improved methods of aonla pricking with women workers.

    Science.gov (United States)

    Rai, Arpana; Gandhi, Sudesh; Sharma, D K

    2012-01-01

    Conventional and improved methods of aonla pricking were evaluated ergonomically on an experiment conducted for 20 minute with women workers. The working heart rate, energy expenditure rate, total cardiac cost of work and physiological cost of work with conventional tools varied from 93-102 beats.min-1, 6-7.5 kJ.min-1, 285-470 beats, 14 -23 beats.min-1 while with machine varied from 96-105 beats.min-1, 6.5-8 kJ.min-1 , 336-540 beats, 16-27 beats.min-1 respectively. OWAS score for conventional method was 2 indicating corrective measures in near future while with machine was 1 indicating no corrective measures. Result of Nordic Musculoskeletal Questionnaire revealed that subjects complaint of pain in back, neck, right shoulder and right hand due to unnatural body posture and repetitive movement with hand tool. Moreover pricking was carried out in improper lighting conditions (200-300 lux) resulting into finger injuries from sharp edges of hand tool, whereas with machine no such problems were observed. Output with machine increased thrice than hand pricking in a given time. Machine was found useful in terms of saving time, increased productivity, enhanced safety and comfort as involved improved posture, was easy to handle and operate, thus increasing efficiency of the worker leading to better quality of life.

  1. [Observation of curative effect of herpes zoster treated with acupuncture based on syndrome differentiation combined with pricking and cupping].

    Science.gov (United States)

    Pan, Hua

    2011-10-01

    To compare the differences of curative effects of herpes zoster treated with acupuncture based on syndrome differentiation combined with pricking and cupping and simple pricking and cupping. Eighty-six cases were randomly divided into an observation group (43 cases) and a control group (43 cases). In observation group, acupoints selection based on syndrome differentiation i.e. Quchi (LI 11), Zusanli (ST 36), Sanyinjiao (SP 6), etc. were selected and pricking and cupping at affected parts were applied, and the cases were classified into damp heat in liver and gallbladder, damp retention and spleen deficiency, and qi deficiency and blood stasis. In control group, all the cases were simplely treated with pricking and cupping at affected parts. The treatment was given once a day, and seven days were made one session. The curative effect was evaluated after 2 courses, and the follow-up was carried on after 1 month. The cured and markedly effective rate was 93.0% (40/43) in observation group, superior to that of 67.4% (29/43) in control group (P 0.05). The cured and markedly effective rate of damp retention and spleen deficiency: 93.8% (15/16) in observation group, superior to that of 60.0% (9/15) in control group (P cupping therapy is high pertinent and effective, the postherpetic neuralgia can be reduced significantly and the curative effect is superior to that of simple pricking and cupping.

  2. Knowledge of parents regarding newborn screening test, after accessing the website “Babies’ Portal” - Heel prick test

    Directory of Open Access Journals (Sweden)

    Caroline Antonelli Mendes

    Full Text Available ABSTRACT Purpose: to assess the knowledge of mothers about the heel prick test, develop contents on this test to make it available on the "Babies’ Portal" website, evaluate and validate the informative material developed. Methods: this study was conducted in three stages, that is, the first stage which is about a descriptive study involving 105 mothers of newborn children before performing the neonatal screening "Heel Prick Test", the second one consisting in the development of the website "Babies’ Portal", and the third stage, the evaluation and validation of this material carried out by 20 parents of children between zero and 36 months old, who underwent the neonatal screening Heel Prick Test by accessing the website “Babies’ Portal”. Results: although the interviewed mothers knew that their children had the right to be tested, they showed no knowledge of the diseases that can be prevented, time of diagnosis, nor the consequences arising from the lack of early diagnosis and treatment. The website creation and validation gathered basic information about the Heel Prick Test, and the participants regarded the content from satisfactory to excellent. Conclusion: it is necessary that families know not only about the procedures their children will undergo, but also the reason they are performed and the consequences of failing in doing so.

  3. Food allergies in children: a comparison of parental reports and skin prick test results

    Directory of Open Access Journals (Sweden)

    Camilia Metadea Aji Savitri

    2018-04-01

    Full Text Available Background Food allergy is common in children and its prevalence is generally on the rise. Imprecise parental reports about reactions to particular foods can lead to unnecessary restrictions. Since children have specific growth requirements, such nutritional restrictions may have disturbing effects on children’s growth and development. Objective To compare parental reports on food reactions to skin prick test results in their children. Method Retrospective, cross sectional study using patient’s medical record data during one-year study period. Data were analyzed manually and statistically, to assess the degree of agreement (Kappa’s coefficient and significance (P. Results We collected data from 154 subjects aged 0-18 years. For every allergen assessed, parents reported more food reactions than positive skin prick test results. Allergy incidence were caused, in order, by cow’s milk and chicken (25.3%, eggs (22.1%, chocolate (20.1%, fruits (14.3%, seafood (13%, and saltwater fish (1.9%. Kappa coefficient are all poor (0.05 except for chicken (P=0.02. Conclusion Most parents tend to overestimate which food cause reactions in their children, as reactions reported were not necessarily allergenic. Therefore, every patient experiencing allergy reactions should undergo skin prick testing to confirm the possibility of allergy.

  4. Sensitivity Comparison of the Skin Prick Test and Serum and Fecal Radio Allergosorbent Test (RAST in Diagnosis of Food Allergy in Children

    Directory of Open Access Journals (Sweden)

    Hamid Reza Kianifar

    2016-05-01

    Full Text Available Background: Diagnosis of food allergy is difficult in children. Food allergies are diagnosed using several methods that include medical histories, clinical examinations, skin prick and serum-specific immunoglobulin E (IgE tests, radio-allergosorbent test (RAST, food challenge, and supervised elimination diets. In this study we evaluated allergies to cow's milk, egg, peanut, and fish in children with suspected food allergies with skin prick tests and serum and feces RAST. Methods: Forty-one children with clinical symptoms of food allergies were enrolled in the study. Skin prick tests and serum and fecal RAST were performed and compared with challenge tests. Results: The most common sites of food allergy symptoms were gastrointestinal (82.9% and skin (48.8%. 100% of the patients responded to the challenge tests with cow’s milk, egg, peanut, and fish. 65% of the patients tested positive with the skin prick test, 12.1% tested positive with serum RAST, and 29.2% tested positive with fecal RAST. Conclusions: The skin prick test was more sensitive than serum or fecal RAST, and fecal RAST was more than twice as sensitive as serum RAST.

  5. Preventing the dental needle prick injury (DNPI) : a new approach to ...

    African Journals Online (AJOL)

    The burden of serious occupational hazards of infectious diseases such as HIV, Hepatitis B and C, Lassa and Ebola Virus diseases which can be contracted through such injuries can be quite costly to the dental health care worker and to the healthcare system in general. Dental needle prick injury (DNPI) prevention devices ...

  6. Thin layer convective solar drying and mathematical modeling of prickly pear peel (Opuntia ficus indica)

    International Nuclear Information System (INIS)

    Lahsasni, Siham; Kouhila, Mohammed; Mahrouz, Mostafa; Idlimam, Ali; Jamali, Abdelkrim

    2004-01-01

    This paper presents the thin layer convective solar drying and mathematical modeling of prickly pear peel. For these purposes, an indirect forced convection solar dryer consisting of a solar air collector, an auxiliary heater, a circulation fan and a drying cabinet is used for drying experiments. Moreover, the prickly pear peel is sufficiently dried in the ranges of 32 to 36 deg. C of ambient air temperature, 50 to 60 deg. C of drying air temperature, 23 to 34% of relative humidity, 0.0277 to 0.0833 m 3 /s of drying air flow rate and 200 to 950 W/m 2 of daily solar radiation. The experimental drying curves show only a falling drying rate period. The main factor in controlling the drying rate was found to be the drying air temperature. The drying rate equation is determined empirically from the characteristic drying curve. Also, the experimental drying curves obtained were fitted to a number of mathematical models. The Midilli-Kucuk drying model was found to satisfactorily describe the solar drying curves of prickly pear peel with a correlation coefficient (r) of 0.9998 and chi-square (χ 2 ) of 4.6572 10 -5

  7. In vitro and in vivo characterization of hazelnut skin prick test extracts

    NARCIS (Netherlands)

    Akkerdaas, Jaap H.; Wensing, Marjolein; Knulst, André C.; Aalberse, Rob C.; Hefle, Susan L.; van Ree, Ronald

    2003-01-01

    RATIONALE: Hazelnut allergy ranks among the most frequently observed food allergies. Clinical symptoms range from the oral allergy syndrome to life threatening anaphylaxis. Diagnosis of hazelnut allergy partially relies on in vivo testing by means of skin prick testing (SPT). The aim of this study

  8. Adsorption-desorption isotherms and heat of sorption of prickly pear fruit (Opuntia ficus indica)

    International Nuclear Information System (INIS)

    Lahsasni, S.; Kouhila, M.; Mahrouz, M.

    2004-01-01

    The equilibrium moisture contents were determined for prickly pear fruit using the gravimetric static method at t=30, 40 and 50 deg. C over a range of relative humidities from 0.05 to 0.9. The sorption curves of prickly pear fruit decreased with increase in temperature at constant relative humidity. The hysteresis effect was observed. The GAB, modified Halsey, modified Chung-Pfost, modified Oswin and modified Henderson models were tested to fit the experimental data. The GAB model was found to be the most suitable for describing the sorption curves. The monolayer moisture content values for the sorption at different temperatures are calculated using a modified BET equation. The isosteric heats of desorption and adsorption of water were determined from the equilibrium data at different temperatures

  9. Adsorption-desorption isotherms and heat of sorption of prickly pear fruit (Opuntia ficus indica)

    Energy Technology Data Exchange (ETDEWEB)

    Lahsasni, S.; Kouhila, M. E-mail: kouhila@hotmail.com; Mahrouz, M

    2004-01-01

    The equilibrium moisture contents were determined for prickly pear fruit using the gravimetric static method at t=30, 40 and 50 deg. C over a range of relative humidities from 0.05 to 0.9. The sorption curves of prickly pear fruit decreased with increase in temperature at constant relative humidity. The hysteresis effect was observed. The GAB, modified Halsey, modified Chung-Pfost, modified Oswin and modified Henderson models were tested to fit the experimental data. The GAB model was found to be the most suitable for describing the sorption curves. The monolayer moisture content values for the sorption at different temperatures are calculated using a modified BET equation. The isosteric heats of desorption and adsorption of water were determined from the equilibrium data at different temperatures.

  10. [Characteristics of allergic conjunctivitis with positive skin prick test].

    Science.gov (United States)

    Yang, S; Jiang, Y; Jin, Y M; Zhang, J Y; Li, Y

    2017-09-11

    Objective: To observe the clinical characteristics of allergic conjunctivitis, and the correlations with skin prick test results. Methods: A retrospective study. Forty patients with positive skin prick test result were included. Patients underwent an ophthalmologic examination to identify their primary presenting signs and symptoms. The allergy types were divided into 5 groups. All dates were analyzed for the dependence, normality and homogeneity of variance. Chi-square test, Mann-Whitney U test, Kruskal-Wallis H test and Spearman correlation analysis were performed accordingly. Results: Among 40 patients, 18(45.0%) had a clinical diagnosis of seasonal allergic conjunctivitis, 14(35.0%) had perennial allergic conjunctivitis, 5(12.5%) had vernal keratoconjunctivitis, and 2(5.0%) had atopic keratoconjunctivits, and 1(2.5%) had giant papillary conjunctivitis. There was no significant difference in the number of symptoms and signs score among different types of allergic conjunctivitis, the score of itching and hyperemia had a positive relationship with the number of positive allergens ( r =0.74, Ptest of the allergen, the more symptoms and signs encountered in terms of severity. Conclusion: Seasonal allergic conjunctivitis was the most prevalent disorder, the most important clinical characteristics of allergic conjunctivitis are itching and conjunctival congestion, the main allergens are dust and pollens, patients may be sensitive to multiple allergens. (Chin J Ophthalmol, 2017, 53: 689-693) .

  11. Performance and Pain Tolerability of Current Diagnostic Allergy Skin Prick Test Devices.

    Science.gov (United States)

    Tversky, Jody R; Chelladurai, Yohalakshmi; McGready, John; Hamilton, Robert G

    2015-01-01

    Allergen skin prick testing remains an essential tool for diagnosing atopic disease and guiding treatment. Sensitivity needs to be defined for newly introduced devices. Our aim was to compare the performance of 10 current allergy skin prick test devices. Single- and multiheaded skin test devices (n = 10) were applied by a single operator in a prospective randomized manner. Histamine (1 and 6 mg/mL) and control diluent were introduced at 6 randomized locations onto the upper and lower arms of healthy subjects. Wheal and flare reactions were measured independently by 2 masked technicians. Twenty-four subjects provided consent, and 768 skin tests were placed. Mean wheal diameter among devices differed from 3.0 mm (ComforTen; Hollister-Stier, Spokane, Wash) to 6.8 mm (UniTest PC; Lincoln Diagnostics, Decatur, Ill) using 1 mg/mL histamine (P Diagnostics, Decatur, Ill; and Sharp-Test; Panatrex, Placentia, Calif) using 6 mg/mL histamine (P pain score of less than 4 on a 10-point visual analog scale. Pain scores were higher among women, but this did not reach statistical significance. The Multi-Test PC and the UniTest PC had the lowest pain scores compared with the other devices. All 10 skin prick test devices displayed good analytical sensitivity and specificity; however, 3 mm cannot arbitrarily be used as a positive threshold. The use of histamine at 1 mg/mL is unacceptable for certain devices but may be preferable for the most sensitive devices. On average, there was no pain score difference between multiheaded and single-head devices. Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  12. Airway allergy and skin reactivity to aeroallergens in Riyadh

    International Nuclear Information System (INIS)

    Almogren, Adel

    2009-01-01

    To determine the pattern of skin prick test reactivity to aeroallergens in patients with asthma and rhinitis (airway allergy) residing in Riyadh region. This is a retrospective cross sectional study based on data analysis of skin prick test results of individuals with clinical diagnosis of airway allergy. Allergy skin prick test result data of 139 Saudi nationals from Riyadh region tested at King Khalid University Hospital between January 2003 and March 2004 was analyzed retrospectively. This group comprised of 53% females and 47% males, with a mean age of 27 +/- 12 years. A set of aeroallergens extracts for both indoor and outdoor allergens including fungal spores was used to test the patients. Seventy-five percent (105) of patients reacted to one or more allergen extracts. The most frequently reacting indoor allergen was house dust mite (77.8%) followed by the cat (33.6%) and cockroach (19.2%). Among the outdoor allergens Prosopis juliflora was tested positive in 72.1%, Bermuda grass in 53.8%, Chenopodium album in 47.1%, Rye grass in 36.5% and Salsola kali in 36.5%. A significant proportion of patients were also found reacting to Moulds (18.2%) and Aspergillus fumigatus (18.2%) extracts. Sensitivity to one or more aeroallergens was common in patients, indicating high level of aeroallergen sensitization in patients with airway allergy residing in Riyadh region. (author)

  13. Thin layer convective solar drying and mathematical modeling of prickly pear peel (Opuntia ficus indica)

    Energy Technology Data Exchange (ETDEWEB)

    Lahsasni, S.; Mahrouz, M. [Unite de Chimie Agroalimentaire (LCOA), Faculte des Sciences Semlalia, Marrakech (Morocco); Kouhila, M.; Idlimam, A.; Jamali, A. [Ecole Normale Superieure, Marrakech (Morocco). Lab. d' Energie Solaire et Plantes Aromatiques et Medicinales

    2004-02-01

    This paper presents the thin layer convective solar drying and mathematical modeling of prickly pear peel. For these purposes, an indirect forced convection solar dryer consisting of a solar air collector, an auxiliary heater, a circulation fan and a drying cabinet is used for drying experiments. Moreover, the prickly pear peel is sufficiently dried in the ranges of 32 to 36 {sup o} C of ambient air temperature, 50 to 60 {sup o}C of drying air temperature, 23 to 34% of relative humidity, 0.0277 to 0.0833 m{sup 3}/s of drying air flow rate and 200 to 950 W/m{sup 2} of daily solar radiation. The experimental drying curves show only a falling drying rate period. The main factor in controlling the drying rate was found to be the drying air temperature. The drying rate equation is determined empirically from the characteristic drying curve. Also, the experimental drying curves obtained were fitted to a number of mathematical models. The Midilli-Kucuk drying model was found to satisfactorily describe the solar drying curves of prickly pear peel with a correlation coefficient (r) of 0.9998 and chi-square ({chi}{sup 2}) of 4.6572 10{sup -5}. (Author)

  14. Phytochemical study of prickly pear from southern Morocco

    Directory of Open Access Journals (Sweden)

    Z. Bouzoubaâ

    2016-06-01

    Full Text Available This work concerns the phytochemical study of the prickly pear pulp’s fruits of two opuntia cultivars; Achefri and Amouslem widely present in two regions of southern Morocco; Arbaa Sahel and Asgherkis that are different in their altitude and annual rainfall. The results of the phytochemical study show that the levels of antioxidants have a non-significant difference between the fruits of the two sites (comparing Amouslem and Achefri in the same site, on the one hand, for the differences due to the variety or cultivar, on the other hand between Amouslem and Achefri from the two sites to show the site effect.

  15. Effect of Mozart music on heel prick pain in preterm infants: a pilot randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Cristina Cavaiuolo

    2015-02-01

    Full Text Available Objective: The aim of this pilot study was to determine the effect of music by Mozart on heel prick procedural pain in premature infants.Background: Painful procedures are routinely performed in the setting of neonatal intensive care unit (NICU. Pain may exert short- and long-term deleterious effects on premature babies. Many non-pharmacological interventions have been proven efficacious for blunting neonatal pain.Study design: Randomized, controlled trial.Methods: The study was carried out in the NICU of the “G. Rummo” Hospital in Benevento, Italy. The sample consisted of 42 preterm infants, with no hearing loss or significant cerebral lesions on cranial ultrasound. They were randomized to receive heel lance during a music condition or a no-music control condition. We set strict criteria for selecting and delivering the music. Baseline and postprocedural heart rate and transcutaneous oxygen saturation were manually recorded. The Premature Infant Pain Profile (PIPP score was used to measure the behavioral response to prick. An unpaired t-test was performed for the intergroup comparisons.Results: There were significant differences between groups on heart rate increase, oxygen saturation reduction and PIPP score following the procedure.Conclusions: Listening to Mozart music during heel prick is a simple and inexpensive tool for pain alleviating in preterm stable neonates.

  16. Hurdle technology applied to prickly pear beverages for inhibiting Saccharomyces cerevisiae and Escherichia coli.

    Science.gov (United States)

    García-García, R; Escobedo-Avellaneda, Z; Tejada-Ortigoza, V; Martín-Belloso, O; Valdez-Fragoso, A; Welti-Chanes, J

    2015-06-01

    The effect of pH reduction (from 6·30-6·45 to 4·22-4·46) and the addition of antimicrobial compounds (sodium benzoate and potassium sorbate) on the inhibition of Saccharomyces cerevisiae and Escherichia coli in prickly pear beverages formulated with the pulp and peel of Villanueva (V, Opuntia albicarpa) and Rojo Vigor (RV, Opuntia ficus-indica) varieties during 14 days of storage at 25°C, was evaluated. RV variety presented the highest microbial inhibition. By combining pH reduction and preservatives, reductions of 6·2-log10 and 2·3-log10 for E. coli and S. cerevisiae were achieved respectively. Due to the low reduction of S. cerevisiae, pulsed electric fields (PEF) (11-15 μs/25-50 Hz/27-36 kV cm(-1)) was applied as another preservation factor. The combination of preservatives, pH reduction and PEF at 13-15 μs/25-50 Hz for V variety, and 11 μs/50 Hz, 13-15 μs/25-50 Hz for RV, had a synergistic effect on S. cerevisiae inhibition, achieving at least 3·4-log10 of microbial reduction immediately after processing, and more than 5-log10 at fourth day of storage at 25°C maintained this reduction during 21 days of storage (P > 0·05). Hurdle technology using PEF in combination with other factors is adequate to maintain stable prickly pear beverages during 21 days/25°C. Significance and impact of the study: Prickly pear is a fruit with functional value, with high content of nutraceuticals and antioxidant activity. Functional beverages formulated with the pulp and peel of this fruit represent an alternative for its consumption. Escherichia coli and Saccharomyces cerevisiae are micro-organisms that typically affect fruit beverage quality and safety. The food industry is looking for processing technologies that maintain quality without compromising safety. Hurdle technology, including pulsed electric fields (PEF) could be an option to achieve this. The combination of PEF, pH reduction and preservatives is an alternative to obtain safe and minimally processed

  17. Frozen fruit skin prick test for the diagnosis of fruit allergy.

    Science.gov (United States)

    Garriga, Teresa; Guilarte, Mar; Luengo, Olga; Guillén, Mercé; Labrador-Horrillo, Moisés; Fadeeva, Tatiana; Sala, Anna; Cardona, Victória

    2010-12-01

    Diagnosis of fruit sensitisation by skin prick test (SPT) is fast and easy to perform. Nevertheless, some fruit is not available throughout the year. Freezing aliquots of these fresh fruits to be defrosted would be a good solution to perform SPT at any time. To compare the reproducibility of SPT with Rosaceae and Cucurbitaceae frozen fruit with fresh and commercial fruit extracts. SPT with the following fruit were performed: apricot, cherry, strawberry, nectarine, Japanese medlar, peach, (peel and pulp), yellow and red plum, melon and watermelon. We compared fresh fruit, commercial extract and fruit which had been frozen at -18 degrees C. Results were read by planimetry (Inmunotek prick-film) after 15 minutes. The study group comprised 48 patients (9 males, 39 females) with a mean age of 31, 6 +/- 2.0 years. Concordance of positive and negative results was extremely high and significant in all cases. Correlation between frozen fruit and commercial extract, frozen fruit and fresh and commercial extract and fresh fruit was statistically significant in all cases except for strawberry. The use of frozen fruit is a valid method, as the performance of the SPT is similar to that of fresh fruit. This enables diagnostic procedures with seasonal fruit at any time of the year.

  18. Identification of low allergenic apple cultivars using skin prick tests and oral food challenges.

    Science.gov (United States)

    Vlieg-Boerstra, B J; van de Weg, W E; van der Heide, S; Kerkhof, M; Arens, P; Heijerman-Peppelman, G; Dubois, A E J

    2011-04-01

    As oral allergy syndrome (OAS) symptoms to apple are frequent, we aimed to identify low allergenic apple cultivars and to validate the prick-to-prick skin prick test (SPT) as a suitable screening method. Sixty-eight apple cultivars were tested by SPTs in 33 Dutch adults with OAS, before and during the birch pollen season in 2006 and 2007, respectively. Three cultivars yielding the largest number of negative SPTs (Elise, Santana and Pink Lady®) and one reference cultivar (Golden Delicious) were subsequently tested by single-blind oral food challenges (SBFC) just after picking in fall 2007 (fresh) and in spring 2008 (stored), outside the birch pollen season and preceded by SPTs. In spring, Santana was replaced by Modi®. In fresh apples, OAS symptoms of Elise, as measured by cumulative scores on a Visual Analogue Scale VASt, were significantly lower than those of Santana, Pink Lady and Golden Delicious (P = 0.021; 0.040 and 0.005, respectively). VASt scores of Santana were significantly lower than those of Golden Delicious (P = 0.049). In stored apples, VASt scores of Elise were significantly lower than that of Golden Delicious (P = 0.038). VASt scores of fresh apples did not differ significantly from stored apples, except in Golden Delicious (spring < fall: P = 0.021). The SPTs did not predict the severity of OAS. SPTs are not useful to assess the allergenicity of apple cultivars. By using SBFC, Elise and Santana were identified as low allergenic apple cultivars in patient with OAS. Our data on the effect of storage are inconclusive. © 2010 John Wiley & Sons A/S.

  19. Effects of salinity, temperature, light and dormancy regulating chemicals on seed germination of salsola drummondii ulbr

    International Nuclear Information System (INIS)

    Rasheed, A.; Hameed, A.; Khan, M.A.; Gul, B.

    2015-01-01

    Salsola drummondii Ulbr. is a perennial halophyte found in salt deserts of southern Balochistan, Pakistan. Experiments were conducted to study the effects of salinity (0, 200, 400, 600, 800 and 1000 mM NaCl), thermoperiod (10/20, 15/25, 20/30 and 25/35 degree C), light (12-h photoperiod and dark) and dormancy regulating chemicals (DRCs) on germination, recovery and viability of the seeds of S. drummondii. Seeds of S. drummondii germinated quickly in distilled water at different temperature regimes and increases in salinity decreased seed germination. Interestingly, few seeds could even germinate in 1000 mM NaCl treatment, which is about twice as high as seawater salinity. Seeds were partially photoblastic and showed relatively higher germination under 12-h photoperiod than in dark. Seeds showed poor recovery of germination from salinity and particularly when germinated in dark. Germination inhibition at high salinity (800 mM NaCl) under 12-h photoperiod was partially alleviated by the exogenous application of different DRCs, particularly fusicoccin. Moreover, all the DRCs, except GA4+7, ameliorated germination of salt stressed seeds under complete darkness and GA4 and fusicoccin were most effective. Our study shows that seeds of S. drummondii are highly tolerant to salinity and variation in temperature but partially photoblastic nature indicate that seeds will not germinate if buried under the soil. Seed germination under saline conditions can be improved by the use of DRCs particularly by application of fusicoccin. (author)

  20. Identification of common allergens for united airway disease by skin prick test

    Directory of Open Access Journals (Sweden)

    Vikas Deep Mishra

    2016-01-01

    Full Text Available Objective: Identification of common allergens by skin prick test in patients of united airway disease. Materials and Methods: Skin prick test was performed in 60 patients of United Airway Disease to identify the common allergens. A total of 62 allergens consisting of 36 types of pollen, 5 fungi, 4 insects, 8 type of dusts, 4 dander, 3 fabrics, Dust mite and Parthenium leaves were tested. Result: Most common allergens were Dust mite (60% followed by Parthenium leaves (45%, insects (18.75%, pollen (14.81%, dust allergens (8.51%, fabrics (8.33%, fungi (5.66%, dander (5%. Most common insect allergens were cockroach (female (30%, cockroach (male (23.33%. Common pollens were Ricinus communis (28.33%, Amaranthus spinosus (28.33%, Parthenium hysterophorus (26.66%, Eucalyptus tereticornis (26.66% and Cynodon dactylon (25%. Common dust allergens were house dust (21.66%, paper dust (11.66% and cotton mill dust (10%. Among fabrics kapok cotton (13.33% showed maximum positivity. Among fungi Aspergillus fumigatus (10% followed by A. niger (6.66% were most common. In animal dander group common ones were cat dander followed by dog dander. Conclusion: In conclusion it can be said that the knowledge drawn by above study will help to treat patients by immunotherapy or avoidance strategy.

  1. Prick test: evolution towards automated reading.

    Science.gov (United States)

    Justo, X; Díaz, I; Gil, J J; Gastaminza, G

    2016-08-01

    The prick test is one of the most common medical methods for diagnosing allergies, and it has been carried out in a similar and laborious manner over many decades. In an attempt to standardize the reading of the test, many researchers have tried to automate the process of measuring the allergic reactions found by developing systems and algorithms based on multiple technologies. This work reviews the techniques for automatic wheal measurement with the aim of pointing out their advantages and disadvantages and the progress in the field. Furthermore, it provides a classification scheme for the different technologies applied. The works discussed herein provide evidence that significant challenges still exist for the development of an automatic wheal measurement system that not only helps allergists in their medical practice but also allows for the standardization of the reading and data exchange. As such, the aim of the work was to serve as guideline for the development of a proper and feasible system. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  2. Developing an Agro-Ecological Zoning Model for Tumbleweed (Salsola kali, as Energy Crop in Drylands of Argentina

    Directory of Open Access Journals (Sweden)

    Falasca Silvia

    2016-12-01

    Full Text Available Salsola kali is considered extremely valuable as an energy crop worldwide because it adapts easily to environments with strong abiotic stresses (hydric, saline and alkaline and produces large amounts of biomass in drylands. This species is categorized as an important weed in Argentina. The aim of this work was to design an agro-ecological zoning model for tumbleweed in Argentina, employing a Geography Information System. Based on the bioclimatic requirements for the species and the climatic data for Argentina (1981–2010 period, an agro-climatic suitability map was drawn. This map was superimposed on the saline and alkaline soil maps delineated by the Food and Agriculture Organization for dry climates, generating the agro-ecological zoning on a scale of 1 : 500 000. This zoning revealed very suitable and suitable cultivation areas on halomorphic soils. The potential growing areas extend from N of the Salta province (approximately 22° S to the Santa Cruz province (50° S. The use of tumbleweed on halomorphic soils under semi-arid to arid conditions, for the dual purpose of forage use and source of lignocellulosic material for bioenergy, could improve agricultural productivity in these lands. Furthermore, it could also contribute to their environmental sustainability, since the species can be used to reclaim saline soils over the years. Based on international bibliography, the authors outlined an agro-ecological zoning model. This model may be applied to any part of the world, using the agro-ecological limits presented here.

  3. Characteristics of prickly lettuce seed oil in relation to methods of extraction.

    Science.gov (United States)

    Ramadan, A A

    1976-01-01

    Samples of seed oil of prickly lettuce (Lactuca Sacriola oleifera) which had been obtained by pressing or by extracting with acetone, ethyl ether, petroleum ether or carbon tetrachloride were analysed for the following parameters: viscosity, saponification number, iodine number, thiocyanogen value, unsaponifiable matter, free fatty acids, peroxide number and fatty acid composition. The different parameters varied in part considerably in relation to the method of production (pressing or solvent extraction) and to the solvent. It is tried to interprete these relationships.

  4. Patch and Prick Tests in Hand Eczema: Results of A Sixty Seven Patient Series

    Directory of Open Access Journals (Sweden)

    Bilge Fettahlıoğlu Karaman

    2017-12-01

    Full Text Available Objective: The patch and prick tests have a place in the management of patients with hand eczema. In this study, we investigated whether some of the clinical features patients with hand eczema could provide us with the predictability of skin test results. Methods: In Çukurova University Faculty of Medicine, 67 consecutive patients with hand eczema; evaluated in terms of duration of disease, morphology and severity. All of the patients were undergoes patch tested with the European Standard Series, and needle testing with routine aeroallergens. Results: Patch test with at least one allergen was positive in 46.3% of the patients; wheras this rate was 23.9% for prick test. The likelihood of having a contact sensitivity of patients complaining of hand eczema for at least three years was statistically more significant [odds ratio (OR 0.9]. Although statistically not significant, it is less likely to be sensitized to patients with keratotic and/or licheniform hand eczema (OR 0.3. The severity of hand eczema was not predictive of patch test, there was no indicator of needle test positivity. Conclusion: We strongly recommend patch testing in all patients with prolonged hand eczema.

  5. [Comparative study of 3 types of lancets for performing prick tests].

    Science.gov (United States)

    Montalvo, A; Martín, S; Mesa, A; Cortés, C; Rodríguez, M; Laso, M T

    1996-01-01

    Skin Prick Test is considered the cutaneous test of choice in rutinary clinical practice as well as in research. In the last 15 years a large number of lancets for puncture test have been developed looking for a higher level of standardisation and reproducibility. We have compared three lancets, two of them, NeoAbelló (NA) and Dome/Hollister Stier (DHS), used in puncture test and a third one, Blood Lancet (BL), in the modified prick test. Twenty-two patients sensitised to Phleum pratense were tested with the three lancets at three extract concentrations and histamine 10 mg/mL in duplicate. We compared their wheal sizes by means of parallel line assay, their reproducibility, frequency of bleeding wheals, the disturbance for the patient and the correlation between specific IgE and skin response. Wheal size was similar for puncture tests (DHS and NA lancets) and significatively lower than BL, being necessary to increase 1.9 times the extract concentration when using DHS and NA lancets to achieve the same wheal size than when using BL lancets (p < 0.01). Reproducibility with histamine was similar with the three lancets while with the extract was somehow better with puncture tests but without statistical significance. Bleeding was significantly more frequent with BL although this did not influence the results. Less pain was obtained with BL. Finally, correlation with specific IgE was slightly significant for NA and very similar for the other two. With these results in mind we think that any of the three lancets is valid for the diagnostic of the allergic patient. Nevertheless, puncture tests are probably the most suitable at a research level.

  6. Proficiency testing of skin prick testers as part of a quality assurance system

    DEFF Research Database (Denmark)

    Malling, Hans-Jørgen; Allesen-Holm, Pernille; Karved, Lisbeth Sys

    2016-01-01

    BACKGROUND: Skin prick test is an important diagnostic procedure in clinical allergy but documentation of the quality is often missing. METHODS: We describe a proficiency system to evaluate staff members in relation to the international recommended reproducibility in terms of coefficient...... of variation (CV 0.85) based on blinded octuplicate histamine testing using histamine 3, 10, 30 and 100 mg/ml. RESULTS: Fourteen trained allergy nurses participated in the proficiency testing. More than 95 % of the nurses, generated coefficient of variation...

  7. A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study.

    Science.gov (United States)

    Fidler, Sarah; Lewis, Heather; Meyerowitz, Jodi; Kuldanek, Kristin; Thornhill, John; Muir, David; Bonnissent, Alice; Timson, Georgina; Frater, John

    2017-10-20

    There is a global need for HIV viral load point-of-care (PoC) assays to monitor patients receiving antiretroviral therapy. UNICORN was the first study of an off-label protocol using whole blood finger-prick samples tested with and without a simple three minute spin using a clinic-room microcentrifuge. Two PoC assays were evaluated in 40 HIV-positive participants, 20 with detectable and 20 with undetectable plasma viral load (pVL) (<20 copies/ml). Using 100 µl finger-prick blood samples, the Cepheid Xpert HIV-1 Viral Load and HIV-1 Qual cartridges were compared with laboratory pVL assessment (TaqMan, Roche). For participants with undetectable viraemia by TaqMan, there was poor concordance without centrifugation with the TaqMan platform with only 40% 'undetectable' using Xpert VL and 25% 'not detected' using the Qual assay. After a 3 minute spin, 100% of samples were undetectable using either assay, showing full concordance with the TaqMan assay. Defining a lower limit of detection of 1000 copies/ml when including a spin, there was 100% concordance with the TaqMan platform with strong correlation (rho 0.95 and 0.94; p < 0.0001 for both assays). When including a simple microcentrifugation step, finger-prick PoC testing was a quick and accurate approach for assessing HIV viraemia, with excellent concordance with validated laboratory approaches.

  8. Modeling the effects of temperature and relative humidity on gas exchange of prickly pear cactus (Opuntia spp.) stems

    NARCIS (Netherlands)

    Guevara-Arauza, J.C.; Yahia, E.M.; Cedeno, L.; Tijskens, L.M.M.

    2006-01-01

    A model to estimate gas profile of modified atmosphere packaged (MAP) prickly pear cactus stems was developed and calibrated. The model describes the transient gas exchange taking in consideration the effect of temperature (T) and relative humidity (RH) on film permeability (FPgas), respiration rate

  9. Phytochemical characterization of different prickly pear (Opuntia ficus-indica (L.) Mill.) cultivars and botanical parts: UHPLC-ESI-MSn metabolomics profiles and their chemometric analysis.

    Science.gov (United States)

    Mena, Pedro; Tassotti, Michele; Andreu, Lucía; Nuncio-Jáuregui, Nallely; Legua, Pilar; Del Rio, Daniele; Hernández, Francisca

    2018-06-01

    Prickly pear is an important source of bioactive compounds. However, a comprehensive characterization of the phytochemical profile of its aerial botanical parts, considering genotypic differences, has not been conducted. This study evaluated the phytochemical composition of four botanical parts (fruit pulp and skin, and young and adult cladodes) of six cultivars. Analysis was carried out by using two non-targeted UHPLC-ESI-MS n experimental conditions and assisted with multivariate analysis to facilitate data interpretation. Up to 41 compounds, mainly (poly)phenolic molecules, were identified and quantified, 23 compounds being reported for the first time in Opuntia ficus-indica. Phenolic composition varied significantly depending on the part of the plant. Betalains were detected only in the fruit of a red cultivar. This study provided novel insights in terms of identification of bioactives and thorough characterization of botanical parts of prickly pears. This information may be used for the development of prickly pear-derived products with high levels of bioactive compounds. Copyright © 2018 Elsevier Ltd. All rights reserved.

  10. Standardization of food allergen extracts for skin prick test

    DEFF Research Database (Denmark)

    Skamstrup Hansen, K; Bindslev-Jensen, C; Skov, P S

    2001-01-01

    The aim of the study was to standardize and evaluate technically optimized food allergen extracts for use in skin prick test (SPT). The standardization procedure comprised 36 allergic histories in 32 food allergic patients with 21 healthy, non-atopic individuals serving as controls. The patients...... had a history of allergic symptoms upon ingestion of either cow's milk (n=3), hen's egg (n=9), wheat (n=4), hazelnut (n=14) or cod (n=6). They also had specific IgE in serum to the food in question and a positive SPT with a fresh preparation of the food. The diagnosis had been confirmed by a double......-blind, placebo-controlled food challenge, except for the hazelnut-allergic patients. The controls were subjected to an open food challenge with all the foods to ensure tolerance. The standardization was performed by means of titrated SPT in accordance with the guidelines on biological standardization from...

  11. ANATOMICAL PROPERTIES AND FIBER DIMENSION OF PRICKLY ACACIA (Acacia nilotica L. FROM BALURAN NATIONAL PARK

    Directory of Open Access Journals (Sweden)

    Krisdianto Krisdianto

    2007-07-01

    Full Text Available Acacia nilotica (L. Willd. ex. Delile growing in Baluran National Park has dramatically altered the ecological balance of grasslands and thereby threatens the existence of local biodiversity. Prickly acacia is able to spread rapidly and remains uncontrollable. Baluran National Park authorization has been struggling to control this prickly acacia trees. One possible action that can be taken to encounter this problem is allowing wood based industries, and local people take advantages of this nilotica timber utilization. This paper studies the anatomical properties and fiber dimensions of nilotica timber and discusses the possible utilization of  nilotica timber.   This timber is characterized by dark brown heartwood which is clearly distinct from reddish brown color of sapwood. The denser cell wall shows attractively streaked in tangential surfaces. The length of  wood fiber decreases from pith toward periphery portion. Longitudinally, higher stem has shorter fiber. Nilotica wood has second class quality of fiber, which means its fiber is moderately thick with narrow lumen diameter. Due to small log diameter and branches, the nilotica timber is not recommended for construction material. The timber is suitable for carved and turnery products. Nilotica timber is suitable for charcoal manufacture and fuel wood due to its high calorific value.

  12. A cephalometric study to determine the plane of occlusion in completely edentulous patients: part I.

    Science.gov (United States)

    Hindocha, Amit D; Vartak, Vikas N; Bhandari, Aruna J; Dudani, Mohit

    2010-12-01

    To determine the relationship between the plane of occlusion and the Camper's line (ala-tragus line). Lateral cephalograms of 105 dentulous subjects were obtained after outlining the tragus and the base of the ala of the nose with radiopaque markers. Tracings of the cephalograms were done and the relationship between the plane of occlusion and the Camper's line (ala-tragus line) was noted. The most common tragal reference as a posterior landmark for determination of plane of occlusion was found to be below inferior (in 30.48% of subjects), and inferior (in 24.76% of subjects). The least common tragal reference was found to be above superior (in 3.82% of subjects) followed by superior of tragus and the point between superior and middle of the tragus (in 6.66% of subjects). The tragal reference in this study population was more towards the inferior of the tragus, with most of the times being below the inferior border. Therefore, the orientation of the plane of occlusion using the superior of tragus as a posterior landmark (according to the widely accepted definition of Camper's line) may be considered to be questionable. Further, the use of the tragus as a posterior landmark for the orientation of the plane of occlusion may be questioned on the basis of the findings of this study.

  13. [Importance of accurate assessment of capillary blood glucose level in diagnosis: misleading results in handlers of prickly pears (Opuntia ficus-indica)].

    Science.gov (United States)

    García Pantoja, José; Landróguez Salinas, Sergio; Barrero Luque, Susana; Pérez Pérez, José Antonio

    2016-01-01

    Hypoglycemia can cause symptoms of neuroglycopenia that can be easily confused with symptoms of greater clinical importance such as stroke. Appropriate differential diagnosis between these 2 processes will be essential for guiding the treatment approach. We report a case in which stroke was initially suspected because hypoglycemia was masked by the incorrect capillary blood glucose (CBG) measurement technique used in treating an itinerant vendor of prickly pears (Opuntia ficus-indica). We studied the effects of prickly pear handling on the CBG levels in 11 vendors before and after they handled the fruit. CBG levels were noticeably higher after the fruit was handled in all cases. Our observations reveal the need to measure both capillary and venous blood glucose levels to rule out hypoglycemia in patients with acute neurological symptoms.

  14. Skin prick test reactivity to aeroallergens by filaggrin mutation status

    DEFF Research Database (Denmark)

    Hougaard, M G; Johansen, J D; Linneberg, A

    2014-01-01

    BACKGROUND: Studies have shown that filaggrin gene (FLG) mutations are positively associated with sensitization to aero allergens. We hypothesized that FLG mutations would also have an effect on the mean size of positive skin prick test (SPT) reactions as well as the number of positive reactions....... OBJECTIVE: To investigate the effect of FLG mutations on the mean size and the number of positive SPT reactions, as well as the association with positive specific IgE. METHODS: A random sample of 3335 adults from the general population in Denmark was genotyped for the R501X and 2282del4 mutations in the FLG...... mutations alone are insufficient to cause secondary sensitization to allergens. The positive association seen in patients must be explained by a combination of further barrier abnormality caused by dermatitis as well as increased allergen exposure....

  15. Bio-cultural anchorage of the prickly pear cactus in Tlalnepantla (Morelos, Mexico

    Directory of Open Access Journals (Sweden)

    Torres-Salcido, Gerardo

    2016-06-01

    Full Text Available The prickly pear cactus is a source of food with strong bio-cultural anchorage in Mexico. This is due to at least three factors: 1 the nature and heritage of cacti; 2 cultural heritage; and 3 the socio-cultural relationships with historical and symbolic roots that have facilitated knowledge of how to cultivate it and how to use it. The aim of this article is to put factors of territorial anchorage and its historical transformation in context by examining the case of the municipality of Tlalnepantla in the state of Morelos, Mexico. This community has experienced accelerated change due to the exchange of traditional crops for the prickly pear cactus and the integration of farming, commercialization and agro-transformation. Our hypothesis is that the market, internal conflicts and a lack of socio-institutional coordination have put social organization into crisis, favoring the territorial spread of the prickly pear cactus and making the Local Agro-Food Systems (LAFS of Tlalnepantla less competitive. The conclusions highlight important economic and social advances whose roots lie in the strengthening and anchorage of the territory-product. However, circumstances both internal and external to the community persist, such as intra-community conflicts, the international market and cultural paradigm shifts that affect the producers and put consolidation of the LAFS at risk.El nopal es un alimento con un fuerte anclaje bio-cultural en México, propiciado por al menos tres factores: 1 la naturaleza y el patrimonio de cactáceas; 2 el patrimonio cultural; y, 3 las relaciones socio-culturales que han permitido un “saber hacer” y un “saber utilizar” con raíces históricas y simbólicas. El objetivo es situar los factores de anclaje territorial y su transformación histórica tomando como caso el municipio de Tlalnepantla, en el estado de Morelos, México. Esta comunidad ha experimentado un acelerado cambio por la reconversión de los cultivos

  16. Topical non-peptide antagonists of sensory neurotransmitters substance P and CGRP do not modify patch test and prick test reactions

    DEFF Research Database (Denmark)

    Wallengren, Joanna; Edvinsson, Lars

    2014-01-01

    developed. Their effect on the skin barrier was measured in terms of transepidermal water loss (TEWL) while permeation was calculated using permeation coefficients. Patch tests in patients allergic to nickel and prick test reactions to histamine were used as models. None of the treatments increased TEWL......, suggesting there to be no impairment of the skin barrier. Permeation coefficients indicated moderate permeation. Histamine prick tests induced a flare with a mean area of 662 + 275 mm(2) and a weal with a mean volume of 49 + 11 mm(3). These reactions as well as histamine-induced pruritus were not affected...... significantly by any of the treatments. Treatment with aprepitant and its vehicle alone resulted in a potentiating of the infiltration of nickel reactions compared with test reactions obtained after no treatment (1147 + 423 mm(3) and 1427 + 566 mm(3) vs 683 +202 mm(3)) (p = 0.03). Telcagepant induced...

  17. Alien plant species list and distribution for Camdeboo National Park, Eastern Cape Province, South Africa

    Directory of Open Access Journals (Sweden)

    Mmoto L. Masubelele

    2009-09-01

    Full Text Available Protected areas globally are threatened by the potential negative impacts that invasive alien plants pose, and Camdeboo National Park (CNP, South Africa, is no exception. Alien plants have been recorded in the CNP since 1981, before it was proclaimed a national park by South African National Parks in 2005. This is the first publication of a list of alien plants in and around the CNP. Distribution maps of some of the first recorded alien plant species are also presented and discussed. To date, 39 species of alien plants have been recorded, of which 13 are invasive and one is a transformer weed. The majority of alien plant species in the park are herbaceous (39% and succulent (24% species. The most widespread alien plant species in the CNP are Atriplex inflata (= A. lindleyi subsp. inflata, Salsola tragus (= S. australis and cacti species, especially Opuntia ficus-indica. Eradication and control measures that have been used for specific problematic alien plant species are described. Conservation implications: This article represents the first step in managing invasive alien plants and includes the collation of a species list and basic information on their distribution in and around the protected area. This is important for enabling effective monitoring of both new introductions and the distribution of species already present. We present the first species list and distribution information for Camdeboo National Park.

  18. Exploitation of Leuconostoc mesenteroides strains to improve shelf life, rheological, sensory and functional features of prickly pear (Opuntia ficus-indica L.) fruit puree.

    Science.gov (United States)

    Di Cagno, Raffaella; Filannino, Pasquale; Vincentini, Olimpia; Lanera, Alessia; Cavoski, Ivana; Gobbetti, Marco

    2016-10-01

    Strains of Leuconostoc mesenteroides were identified from raw prickly pear (Opuntia ficus-indica L.). Five autochthonous strains were selected based on the kinetics of growth and acidification on prickly pear fruit juice, and the capacity to synthesize exo-polysaccharides. All selected Leuc. mesenteroides strains showed an in vitro mucilage-degrading capability. A protocol for processing and storage of fermented prickly pear fruit puree (FP) was set up. Unstarted FP and chemically acidified FP were used as the controls. Starters grew and remained viable at elevated cell numbers during 21 days of storage at 4 °C. Contaminating Enterobacteriaceae and yeasts were found only in the controls. Viscosity and serum separation distinguished started FP compared to the controls. Colour parameters, browning index, sensory attributes, antimicrobial activity, vitamin C and betalains levels were positively affected by lactic acid fermentation. Increase of free radical scavenging activity in ethyl acetate soluble extract suggested an effect of selected strains on phenolic profiles. Started FP markedly inhibited the inflammatory status of Caco-2/TC7 cells, and also contributed to maintaining the integrity of tight junctions. Started FP scavenged the reactive oxygen species generated by H2O2 on Caco-2 cells. All selected strain variously affected the immunomodulatory activity towards anti- and pro-inflammatory cytokines. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Food Sensitivity in Children with Acute Urticaria in Skin Prick Test: Single Center Experience

    Directory of Open Access Journals (Sweden)

    Hatice Eke Gungor

    2015-11-01

    Full Text Available Aim: Families of children with acute urticaria often think that there is food allergy in children with urticaria and insist for skin tests. In this study, it was aimed to determine whether skin prick tests are necessary in cases presented with acute urticaria, in whom other causes of acute urticaria are excluded. Material and Method: A test panel involving cow milk, egg white, wheat, hazelnut, peanut, soybean, walnut, sesame, and tuna fish antigens was applied to the children presented with acute urticaria between 1 August 2013 and 1 August 2014, in whom other causes of acute urticaria were excluded and suspected food allergy was reported by parents. Results: Overall, 574 children aged 1-14 years were included to the study. Of the patients, sensitization against at least one food antigen was detected in 22.3% (128/574 of the patients. This rate was found to be 31.9% among those younger than 3 years, while 19.3% in those older than 3 years. Overall, sensitization rates against food allergen in panel were as follows: egg white, 7.3%; wheat, 3.3%; cow milk, 2.7%,; sesame, 2.8%; hazelnut, 2.4%; soybean, 2.3%; peanut, 1.9%, walnut, 1.6%; tuna fish, 1.6%. In general, the history of patients wasn%u2019t compatible with food sensitization detected. Discussion: Sensitization to food allergens is infrequent in children presented with acute urticaria, particularly among those older than 3 years despite expressions of parent and skin prick tests seems to be unnecessary unless strongly suggestive history is present.

  20. IgE Sensitization Patterns to Commonly Consumed Foods Determined by Skin Prick Test in Korean Adults

    OpenAIRE

    Kim, Sung Ryeol; Park, Hye Jung; Park, Kyung Hee; Lee, Jae-Hyun; Park, Jung-Won

    2016-01-01

    Offending food allergens can vary with regional preferences in food consumption. In this study, we analysed sensitization rates to commonly consumed foods in Korean adults suspected of having food allergy. One hundred and thirty four subjects underwent a skin prick test (SPT) with 55 food allergens, of which 13 were made by our laboratory and the rest were commercially purchased. Of the 134 patients, 73 (54.5%) were sensitized to one or more food allergens. Sensitization to chrysalis was dete...

  1. Sensitization to cereals and peanut evidenced by skin prick test and specific IgE in food-tolerant, grass pollen allergic patients

    DEFF Research Database (Denmark)

    Martens, Maria; Schnoor, Heidi J; Malling, Hans-Jørgen

    2011-01-01

    The botanical relation between grass and cereal grains may be relevant when diagnosing food allergy to cereals. The aim was to investigate the diagnostic specificity of skin prick test (SPT) and specific immunoglobulin E (sIgE) tests to cereals and peanut in grass pollen allergic subjects without...

  2. The Effect of Different Treatments on Seed Dormancy Breaking of Weed Stalked Bur Grass Tragus racemosus (L. AlI.

    Directory of Open Access Journals (Sweden)

    Gholam reza doraki

    2017-01-01

    Full Text Available Introduction: Stalked Bur Grass (Tragus racemosus L. is an angiosperm annual plant with C4 photosynthesis pathway and stolon. It grows in hot and dry summers. This plant spreaded throughout the world from hot regions of Africa. It is regularly seen in barren lands or in between the generations with sequential initial stages with light-texture soils. Seed dormancy is in fact a physiological phenomenon which is observed in the seeds of most crops, pasture plants, medicinal herbs and weeds. Dormancy allows the plant to guarantee its germination and survival for long years and to survive through adverse environmental conditions through its spatial and temporal spread. Materials and Methods: In order to evaluation dormancy break of Tragus racemosus L. seeds, an experiment was carried out based a Randomized Complete Block Design with four replications in research laboratory of Department of Agriculture, Birjand University during 2013. The initial experiments showed that the seeds of Stalked Bur Grass had initial dormancy and were unable to germinate at normal conditions, so that less than 5% of the seeds germinated. The studied treatments for breaking seeds dormancy included control (seeds disinfection by distilled water, wet chilling at 4°C for 1, 2, 3 and 4 weeds, treatment with H2SO4 at 97% for 20, 40, 60 and 80 seconds, treatment with KNO3 at 0.2, 0.4, 0.6 and 0.8% for 24 hours and treatment with Gibberellic acid (GA3 at 50, 100, 200 and 400 ppm. In this study, 25 seeds of Stalked Bur Grass were uniformly placed in petri dishes with the diameters of 9 cm on Watmann filter papers and were applied with 5 mL distilled water. The number of germinated seeds was counted on a daily basis for 21 days. In the end, germination percentage and rate was determined. Results and Discussion: The results revealed that the effect of all studied levels of all treatments were significant on germination percentage and rate. The highest germination percentage (76% was

  3. Evaluation of the halophyte Salsola soda as an alternative crop for saline soils high in selenium and boron.

    Science.gov (United States)

    Centofanti, Tiziana; Bañuelos, Gary

    2015-07-01

    Urbanization, industrial development, and intensive agriculture have caused soil contamination and land degradation in many areas of the world. Salinization is one important factor contributing to land degradation and it affects agricultural production and environmental quality. When salinization is combined with soil pollution by trace elements, as it occurs in many arid and semi-arid regions around the world, strategies to phyto-manage pollutants and sustain crop production need to be implemented. In this study, we present the case of saline soils in the West side of Central California which contain naturally-occurring selenium (Se), boron (B), and other salts, such as NaCl, CaCl2, Na2SO4, and Na2SeO4. To sustain crop production on Se- and B-laden arid saline soils, we investigated the potential of the halophyte "agretti" (Salsola soda L.) as an alternative crop. The aim of our greenhouse study was to examine adaptability, B tolerance, and Se accumulation by S. soda grown on soils collected from a typical saline-laden field site located on the West side of the San Joaquin Valley (SJV). Our results showed that S. soda tolerates the saline (EC ∼ 10 dS m(-1)) and B-laden soils (10 mg B L(-1)) of the SJV even with the additional irrigation of saline and B rich water (EC ∼ 3 dS m(-1) and 4 mg B L(-1)). Under these growing conditions, the plant can accumulate high concentrations of Na (80 g Na kg(-1) DW), B (100 mg B kg(-1) DW), and Se (3-4 mg Se kg(-1) DW) without showing toxicity symptoms. Hence, S. soda showed promising potential as a plant species that can be grown in B-laden saline soils and accumulate and potentially manage excessive soluble Se and B in soil. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Knowledge of and attitudes toward heel prick screening for sickle cell disease in Saint Lucia.

    Science.gov (United States)

    Alexander, Sonia; Belmar-George, Sharon; Eugene, Alisha; Elias, Vanessa

    2017-06-08

    In the Caribbean country of Saint Lucia, umbilical-cord-blood screening for sickle cell disease (SCD) was the testing method that health care workers (HCWs) on the maternity wards of the hospitals preferred until the new heel prick (HP) testing method was introduced in the country in 2014. This SCD study sought to assess HCWs' knowledge of and attitude toward HP screening and also determine new mothers' favorability toward HP screening. A total of 70 HCWs and 132 new mothers answered survey questionnaires in three hospitals. In addition, four focus group discussions were held, two with HCWs and two with the mothers. Among the HCWs interviewed, 85.7% of them had knowledge of the HP test. However, only 25.7% had attended training sessions on the procedure. Among the HCWs, 64.3% of them felt the HP test should be mandatory, 27.1% said it should not be mandatory, and 8.6% did not know if it should be mandatory. In their focus groups, the HCWs said they believed the mothers would accept the HP method. For their part, 22.0% of the mothers said they had heard about the HP test, and 63.6% reported knowing the reason why the baby would be tested. Further, 83.3% indicated that the test would be beneficial for the baby. In addition, 88.6% of the mothers said that more information on the HP test was needed. In their focus group discussions, the mothers said they were generally not concerned about the pain the heel prick method might cause the baby. The HCWs' knowledge of the HP screening method was high. The mothers trust HCWs, and the mothers would accept the HP procedure irrespective of their knowledge of the test and any discomfort associated with this screening method.

  5. Knowledge of and attitudes toward heel prick screening for sickle cell disease in Saint Lucia

    Directory of Open Access Journals (Sweden)

    Sonia Alexander

    2017-06-01

    Full Text Available ABSTRACT Objectives In the Caribbean country of Saint Lucia, umbilical-cord-blood screening for sickle cell disease (SCD was the testing method that health care workers (HCWs on the maternity wards of the hospitals preferred until the new heel prick (HP testing method was introduced in the country in 2014. This SCD study sought to assess HCWs’ knowledge of and attitude toward HP screening and also determine new mothers’ favorability toward HP screening. Methods A total of 70 HCWs and 132 new mothers answered survey questionnaires in three hospitals. In addition, four focus group discussions were held, two with HCWs and two with the mothers. Results Among the HCWs interviewed, 85.7% of them had knowledge of the HP test. However, only 25.7% had attended training sessions on the procedure. Among the HCWs, 64.3% of them felt the HP test should be mandatory, 27.1% said it should not be mandatory, and 8.6% did not know if it should be mandatory. In their focus groups, the HCWs said they believed the mothers would accept the HP method. For their part, 22.0% of the mothers said they had heard about the HP test, and 63.6% reported knowing the reason why the baby would be tested. Further, 83.3% indicated that the test would be beneficial for the baby. In addition, 88.6% of the mothers said that more information on the HP test was needed. In their focus group discussions, the mothers said they were generally not concerned about the pain the heel prick method might cause the baby. Conclusions The HCWs’ knowledge of the HP screening method was high. The mothers trust HCWs, and the mothers would accept the HP procedure irrespective of their knowledge of the test and any discomfort associated with this screening method.

  6. Determination of some mineral contents of prickly pear (Opuntia ficus-indica L.) seed flours.

    Science.gov (United States)

    Al-Juhaimi, Fahad; Özcan, Mehmet Musa

    2013-05-01

    The aim of this study was to determine some mineral contents of prickly pear (Opuntia fıcus-indica L.) seeds collected from different locations. The mineral contents of seeds were established by inductively coupled plasma atomic emission spectrometry. All the seeds contained Ca, K, Mg and P at high levels. Calcium content ranged between 268.5 (sample no. 11) and 674.8 ppm (sample no. 4). The level of K changed between 346.7 (sample no. 1) and 676.1 ppm (sample no. 13). Phosphorus content of seeds varied between 1,173.6 (sample no. 14) and 1,871.3 ppm (sample no. 1). It is apparent that seeds are good sources of the macro and micro minerals and can be consumed as a food ingredient to provide nutrition.

  7. Estimation of optimal nasotracheal tube depth in adult patients.

    Science.gov (United States)

    Ji, Sung-Mi

    2017-12-01

    The aim of this study was to estimate the optimal depth of nasotracheal tube placement. We enrolled 110 patients scheduled to undergo oral and maxillofacial surgery, requiring nasotracheal intubation. After intubation, the depth of tube insertion was measured. The neck circumference and distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch were measured. To estimate optimal tube depth, correlation and regression analyses were performed using clinical and anthropometric parameters. The mean tube depth was 28.9 ± 1.3 cm in men (n = 62), and 26.6 ± 1.5 cm in women (n = 48). Tube depth significantly correlated with height (r = 0.735, P < 0.001). Distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch correlated with depth of the endotracheal tube (r = 0.363, r = 0.362, and r = 0.546, P < 0.05). The tube depth also correlated with the sum of these distances (r = 0.646, P < 0.001). We devised the following formula for estimating tube depth: 19.856 + 0.267 × sum of the three distances (R 2 = 0.432, P < 0.001). The optimal tube depth for nasotracheally intubated adult patients correlated with height and sum of the distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch. The proposed equation would be a useful guide to determine optimal nasotracheal tube placement.

  8. Movement Images and Existential Problematics in the Novel by S. Beckett “More Pricks than Kicks”

    Directory of Open Access Journals (Sweden)

    Il’ya N. Chernyshev

    2017-06-01

    Full Text Available The article considers the semantic prerequisites for the use of images associated with movement in S. Beckett’s novel «More Pricks than Kicks» and their functioning in the context of the existential problematic of the work. A great influence on the author’s early prose was made by the modernist tradition and, in particular, the work of J. Joyce. Numerous allusions and a complex system of characters, the distinguishing features of which are the peculiarities of their movement, allowed the writer to express his views on the problem of human existence. This study suggests looking at the figurative component of S. Beckett’s early work from the viewpoint of phenomenological analysis.

  9. Functional and Aesthetic Tragal Reconstruction in the Age of Mobile Electronic Devices

    Directory of Open Access Journals (Sweden)

    Colleen F. Perez

    2016-01-01

    Full Text Available We present a method to create a tragus using the patient’s conchal cartilage. It is a simplified, single-stage technique with well-hidden incisions, yet it maintains the rigidity of a natural tragus. This patient did not have a history of radiation to the area, which may compromise healing with this technique. The cosmetic importance of the tragus has been described, but its functionality in accommodating modern technology has not been previously discussed. The main treatment goal for this patient was to gain the ability to wear earphones (clinical question/level of evidence: therapeutic, V.

  10. Secondary invasions of noxious weeds associated with control of invasive Tamarix are frequent, idiosyncratic and persistent

    Science.gov (United States)

    González, Eduardo; Sher, Anna A.; Anderson, Robert M.; Bay, Robin F.; Bean, Daniel W.; Bissonnete, Gabriel J.; Cooper, David J.; Dohrenwend, Kara; Eichhorst, Kim D.; El Waer, Hisham; Kennard, Deborah K.; Harms-Weissinger, Rebecca; Henry, Annie L.; Makarick, Lori J.; Ostoja, Steven M.; Reynolds, Lindsay V.; Robinson, W. Wright; Shafroth, Patrick B.; Tabacchi, Erich

    2017-01-01

    Control of invasive species within ecosystems may induce secondary invasions of non-target invaders replacing the first alien. We used four plant species listed as noxious by local authorities in riparian systems to discern whether 1) the severity of these secondary invasions was related to the control method applied to the first alien; and 2) which species that were secondary invaders persisted over time. In a collaborative study by 16 research institutions, we monitored plant species composition following control of non-native Tamarix trees along southwestern U.S. rivers using defoliation by an introduced biocontrol beetle, and three physical removal methods: mechanical using saws, heavy machinery, and burning in 244 treated and 79 untreated sites across six U.S. states. Physical removal favored secondary invasions immediately after Tamarix removal (0–3 yrs.), while in the biocontrol treatment, secondary invasions manifested later (> 5 yrs.). Within this general trend, the response of weeds to control was idiosyncratic; dependent on treatment type and invader. Two annual tumbleweeds that only reproduce by seed (Bassia scoparia and Salsola tragus) peaked immediately after physical Tamarix removal and persisted over time, even after herbicide application. Acroptilon repens, a perennial forb that vigorously reproduces by rhizomes, and Bromus tectorum, a very frequent annual grass before removal that only reproduces by seed, were most successful at biocontrol sites, and progressively spread as the canopy layer opened. These results demonstrate that strategies to control Tamarix affect secondary invasions differently among species and that time since disturbance is an important, generally overlooked, factor affecting response.

  11. [Effect of joss stick moxibustion combined with pricking and cupping for acute herpes zoster and its mechanism of analgesia].

    Science.gov (United States)

    Ye, Guoping; Su, Meiling; Zhu, Dingyu; Zhang, Linyun; Lin, Wang; Huang, Li; Wu, Mingxia

    2017-12-12

    To observe the effects of conventional western medication and joss stick moxibustion combined with pricking and cupping for herpes zoster in acute stage, and to explore its analgesic mechanism. Seventy patients with acute herpes zoster were randomized into an observation group (33 cases after 2 dropping) and a control group (34 cases after 1 dropping). Patients in the observation group were treated with joss stick moxibustion combined with pricking and cupping at local ashi points for 7 times, once every other day. Oral acyclovir, vitamin B 1 and mecobalamin tablets were applied in the control group for continuous 14 days, and interferon injection was used for continuous 6 days, etc. The herpes evaluation indexes of blister stopping time, scab time and decrustation time as well as pain intensity were observed before and after treatment. Peripheral serum substance P (SP) content of herpes local situation was detected. The comprehensive effects were evaluated. The blister stopping time, scab time and decrustation time in the observation group were shorter than those in the control group (all P 0.05). The pain beginning to ease time and duration time in the observation group were better than those in the control group (both P 0.05). The cured rate of the observation group was better than that of the control group [66.7% (22/33) vs 58.8% (20/34), P cupping are effective for herpes zoster, which have quicker and good analgesic effects than conventional western medication. Its mechanism may be related to reducing the content of SP more fast and to a larger degree.

  12. [A cephalometric study on determining the orientation of occlusal plane].

    Science.gov (United States)

    Xie, J; Zhao, Y; Chao, Y; Luo, W

    1993-12-01

    A study of the parallel relationship between the occlusal plane and the line connecting nasal alar and tragus was made in 90 dentulous cases by using cephalometry. The results show that the line connecting the inferior point of nasal alar and the mid-point of tragus runs much more parallel with the occlusal plane. The regression equation reveals a "line of closest fitting". It was used in the prosthetic treatment for 50 edentulous patients with good clinical results. The line connecting the inferior point of nasal alar and the mid-point of tragus therefore represents a proper reference plane for determining occlusal plane and hence should be still a valuable index in clinical dentistry.

  13. Fonoaudiologia e estética facial: estudo de casos Speech therapy and facial esthetic: cases studies

    Directory of Open Access Journals (Sweden)

    Carolina Paes

    2007-06-01

    Full Text Available OBJETIVO: caracterizar as modificações presentes no comportamento facial após tratamento fonoaudiológico proposto para face, através da comparação das medidas da projeção do sulco nasogeniano ao tragus pré e pós-tratamento fonoaudiológico MÉTODOS: a população foi constituída de 10 professores atendidos no Hospital dos Servidores do Estado de Pernambuco de ambos os sexos, e com idade entre 33 e 63 anos. O processo de coleta de dados consistiu de protocolos de anamnese e avaliação, protocolo de desempenho, além do registro fotográfico. Foi realizada a medida da projeção do sulco nasogeniano ao tragus. Durante dezesseis sessões foram trabalhados exercícios isotônicos, isométricos, isocinéticos, massagens, manipulações faciais, equilíbrio funcional estomatognático, além de orientações aos cuidados faciais. RESULTADOS: foi constatada uma redução das medidas da projeção do sulco nasogeniano ao tragus em ambos os lados, observando-se também um notório equilíbrio entre os lados direito e esquerdo. CONCLUSÃO: o programa de tratamento proposto para face reduziu as medidas da projeção do sulco nasogeniano ao tragus e proporcionou um equilíbrio entre essas medidas.PURPOSE: to describe the modifications in the face behavior after the speech language pathology treatment proposed for the face, by comparing the measurements of the projection related to the nasolabial sulcus to the tragus of these patients before and after the speech language pathology treatment. METHODS: the surveyed population was mad up by 10 teachers treated at the "Hospital dos Servidores do Estado" aged between 33 and 63 years. Data collection process was made up by protocols, anamnesis and evaluation, performance protocol, in addition to the photographic record. A measurement of the nasolabial sulcus to the tragus was carried out. Throughout the 16-session, we carried on specific orientation about face isotonic, isometric and isokinetic

  14. Diagnostic value of scratch-chamber test, skin prick test, histamine release and specific IgE in birch-allergic patients with oral allergy syndrome to apple

    DEFF Research Database (Denmark)

    Osterballe, M; Scheller, R; Stahl Skov, P

    2003-01-01

    BACKGROUND: The aim of the study was to examine the diagnostic value of skin prick test (SPT), scratch-chamber test (SCT), histamine release (HR) and specific immunoglobulin E (IgE) in birch-allergic patients with oral allergy syndrome to apple. METHODS: Ten birch-allergic patients with oral...... a detailed case history about symptoms of oral allergy syndrome combined with a SPT with fresh apple peel or A72 will be useful....

  15. A Bayesian analysis of gene flow from crops to their wild relatives: cultivated (Lactuca sativa L.) and prickly lettuce (L. serriola L.) and the recent expansion of L. serriola in Europe

    NARCIS (Netherlands)

    Uwimana, B; D'andrea, L.; Felber, F.; Hooftman, D.A.P.; den Nijs, H.C.M.; Smulders, M.J.M.; Visser, R.G.F.; van de Wiel, C.C.M.

    2012-01-01

    Interspecific gene flow can lead to the formation of hybrid populations that have a competitive advantage over the parental populations, even for hybrids from a cross between crops and wild relatives. Wild prickly lettuce (Lactuca serriola) has recently expanded in Europe and hybridization with the

  16. Isolated endogenous Fusarium endophthalmitis in an immunocompetent adult after a thorn prick to the hand.

    Science.gov (United States)

    Milligan, Alice L; Gruener, Anna M; Milligan, Iain D; O'Hara, Geraldine A; Stanford, Miles R

    2017-06-01

    To report the case of an immunocompetent adult presenting with endogenous Fusarium endophthalmitis. A woman in her thirties presented with symptoms and signs of a unilateral anterior uveitis. After initial improvement with topical corticosteroids, she continued to develop a panuveitis with an associated drop in vision to counting fingers. A vitreous biopsy confirmed Fusarium solani by 18S rRNA fungal gene detection and PCR sequencing. Despite treatment with pars plana vitrectomy, intravitreal amphotericin B and systemic voriconazole her visual outcome was poor. Detailed review of her antecedent history revealed the route of acquisition to be a thorn prick to the hand two weeks prior to presentation. This patient's endophthalmitis most likely resulted from cutaneous inoculation of Fusarium solani with subsequent hematogenous spread. Endogenous Fusarium endophthalmitis is well recognized in the immunocompromised but is very rarely seen in the immunocompetent. This case highlights the importance of thorough history-taking and consideration of fungal endophthalmitis in the differential diagnosis of a treatment-refractory uveitis.

  17. A study of ′cough trick′ technique in reducing vaccination prick pain in adolescents

    Directory of Open Access Journals (Sweden)

    Vikram S. Kumar

    2014-01-01

    Full Text Available Background: The ′cough trick′ (CT technique is used in reducing intramuscular prick (IMP pain during vaccinations and also for brief painful procedures like subcutaneous injection, intravenous cannulation, and so forth. We present the utility study of this technique in male adolescents. Materials and Methods: A Randomized Crossover Volunteer Study of 50 early adolescent male children (age 11-13 receiving immunizations was performed. Participants were recruited from four outpatient pediatric clinics. The strategy required a single "warm-up" cough of moderate force, followed by a second cough that coincided with needle puncture. The principle outcome was self-reported pain. Results: Paired ′t′ test revealed that the procedure was effective at a statistically and clinically significant level for participants. Children found the procedure acceptable and effective. Conclusions: The results of this study suggest that the CT can be an effective strategy for the reduction of pain for male adolescent children undergoing routine immunizations. However, additional research is needed with a larger sample size with different age groups and also including girl children.

  18. The evaluation of fifteen spineless prickly pear cultivars (Opuntia ficus-indica (L. Mill. for fresh fruit production in the Pretoria region

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    A. B. Wessels

    1992-07-01

    Full Text Available Fifteen spineless prickly pear cultivars (Opuntia ficus-indica were evaluated for three successive seasons at the University of Pretoria Research Farm. Yields were recorded and external and internal fruit quality factors were identified and considered. Following this investigation, minimum standards were suggested and the cultivars under review were compared. The cultivars Morado, Algerian, Fusicaulis van Heerden, Fresno, Mexican and Nudosa met all or most of the standards set. It is concluded that for fresh fruit production these cultivars are best suited for planting in the Pretoria region and in areas with similar climatic conditions.

  19. Conditioning pain stimulation does not affect itch induced by intra-epidermal histamine pricks but aggravates neurogenic inflammation in healthy volunteers

    DEFF Research Database (Denmark)

    Andersen, Hjalte Holm; Imai, Yosuke; Petersen, Kristian Kjær

    2016-01-01

    This study investigated whether itch induced by intra-epidermal histamine is subjected to modulation by a standardized conditioned pain modulation (CPM) paradigm in 24 healthy volunteers. CPM was induced by computer-controlled cuff pressure algometry and histamine was introduced to the volar...... forearm by skin prick test punctures. Moreover, neurogenic inflammation and wheal reactions induced by histamine and autonomic nervous system responses (heart rate variability and skin conductance) were monitored. CPM did not modulate the intensity of histamine-induced itch suggesting that pruriceptive...... signaling is not inhibited by pain-recruited endogenous modulation, however, CPM was found to aggravate histamine-induced neurogenic inflammation, likely facilitated by efferent sympathetic fibers....

  20. Effect of Environmental and Behavioral Interventions on Pain Intensity in Preterm Infants for Heel Prick Blood Sampling in the Neonatal Intensive Care Unit.

    Science.gov (United States)

    Baharlooei, Fatemeh; Marofi, Maryam; Abdeyazdan, Zahra

    2017-01-01

    Recent researches suggest that preterm infants understand pain and stress. Because of the wide range of effects of pain on infants, the present study was conducted on the effect of environmental and behavioral interventions on pain due to heel-prick blood sampling in preterm infants. A clinical trial was conducted among 32 infants with gestational age of 32-37 weeks in the intervention and control groups. The effects of noise reduction by earplugs, light reduction by blindfolds, reduction of nursing manipulation, and creation of intrauterine position for neonates, 30 minutes before taking blood samples until 30 minutes after it, were measured during the intervention stage. Data were collected using the Neonatal Infant Pain Scale (NIPS) in 5 stages (before intervention, 2 minutes before sampling, during the sampling, and 5 minutes and 30 minutes after the sampling). The data were analyzed using analysis of variance (ANOVA) and paired t -test in SPSS software. The paired t -test results showed no significant differences between the control and intervention stages in terms of pain scores at base time ( P = 0.42) and 2 minutes before sampling ( P = 0.12). However, at the sampling time ( P = 0.0), and 5 minutes ( P = 0.001) and 30 minutes after the sampling ( P = 0.001), mean pain score in the intervention stage was significantly less than that in the control stage. Based on the findings, environmental and behavioral interventions reduced pain and facilitated heel-prick blood sampling in preterm infants.

  1. Two novel Fusarium species that cause canker disease of prickly ash (Zanthoxylum bungeanum) in northern China form a novel clade with Fusarium torreyae.

    Science.gov (United States)

    Zhou, Xue; O'Donnell, Kerry; Aoki, Takayuki; Smith, Jason A; Kasson, Matthew T; Cao, Zhi-Min

    2016-01-01

    Canker disease of prickly ash (Zanthoxylum bungeanum) has caused a decline in the production of this economically important spice in northern China in the past 25 y. To identify the etiological agent, 38 fungal isolates were recovered from symptomatic tissues from trees in five provinces in China. These isolates were identified by conducting BLASTN queries of NCBI GenBank and phylogenetic analyses of DNA sequence data from the nuclear ribosomal internal transcribed spacer region (ITS rDNA), a portion of the translation elongation factor 1-α (TEF1) gene, and genes encoding RNA polymerase II largest (RPB1) and second largest (RPB2) subunits. Results of these analyses suggested that 30/38 isolates belonged to two novel fusaria most closely related to the Florida torreya (Torreya taxifolia Arn.) pathogen, Fusarium torreyae in Florida and Georgia. These three canker-inducing tree pathogens form a novel clade within Fusarium here designated the F. torreyae species complex (FTOSC). BLASTN queries of GenBank also revealed that 5/38 isolates recovered from cankers represented an undescribed phylogenetic species within the F. solani species complex (FSSC) designated FSSC 6. Stem inoculations of three fusaria on Z. bungeanum resulted in consistent canker symptoms from which these three fusaria were recovered. The two novel fusaria, however, induced significantly larger lesions than FSSC 6. Herein, the two novel prickly ash pathogens are formally described as F. zanthoxyli and F. continuum. © 2016 by The Mycological Society of America.

  2. Use of finger-prick dried blood spots (fpDBS) and capillary electrophoresis for carbohydrate deficient transferrin (CDT) screening in forensic toxicology.

    Science.gov (United States)

    Bertaso, Anna; Sorio, Daniela; Vandoros, Anthula; De Palo, Elio F; Bortolotti, Federica; Tagliaro, Franco

    2016-10-01

    Continued progress in chronic alcohol abuse investigation requires the development of less invasive procedures for screening purposes. The application of finger-prick and related dried blood spots (fpDBS) for carbohydrate deficient transferrin (CDT) detection appears suitable for this aim. Therefore, the goal of this project was to develop a screening method for CDT using fpDBS with CZE analysis. Blood samples prepared by finger-prick were placed on DBS cards and left to air dry; each dried fpDBS disc was shredded into small pieces and suspended in acid solution (60 μL of HCl 120 mmol/L). After centrifugation (10 min at 1500 × g), the collected sample was adjusted to pH 3.5. After an overnight incubation, the pH was neutralised and an iron rich solution was added. After 1 h, CZE analysis was carried out. A group of 47 individuals was studied. Parallel serum samples were collected from each investigated subject and the %CDT for each sample was measured using HPLC and CZE techniques. The fpDBS transferrin sialo isoform electropherograms were similar to those obtained with serum. Moreover, fpDBS CZE CDT percentage levels demonstrated significant statistical correlation with those obtained from serum for both HPLC and CZE %CDT (p < 0.01; r 2 = 0.8913 and 0.8976, respectively), with %CDT from 0.8 to 13.7% for fpDBS and from 0.7 to 12.7% for serum. The newly developed fpDBS procedure for CDT analysis provides a simple and inexpensive tool for use in population screening. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Effect of environmental and behavioral interventions on pain intensity in preterm infants for heel prick blood sampling in the neonatal intensive care unit

    Directory of Open Access Journals (Sweden)

    Fatemeh Baharlooei

    2017-01-01

    Full Text Available Background: Recent researches suggest that preterm infants understand pain and stress. Because of the wide range of effects of pain on infants, the present study was conducted on the effect of environmental and behavioral interventions on pain due to heel-prick blood sampling in preterm infants. Materials and Methods: A clinical trial was conducted among 32 infants with gestational age of 32–37 weeks in the intervention and control groups. The effects of noise reduction by earplugs, light reduction by blindfolds, reduction of nursing manipulation, and creation of intrauterine position for neonates, 30 minutes before taking blood samples until 30 minutes after it, were measured during the intervention stage. Data were collected using the Neonatal Infant Pain Scale (NIPS in 5 stages (before intervention, 2 minutes before sampling, during the sampling, and 5 minutes and 30 minutes after the sampling. The data were analyzed using analysis of variance (ANOVA and paired t-test in SPSS software. Results: The paired t-test results showed no significant differences between the control and intervention stages in terms of pain scores at base time (P = 0.42 and 2 minutes before sampling (P = 0.12. However, at the sampling time (P = 0.0, and 5 minutes (P = 0.001 and 30 minutes after the sampling (P = 0.001, mean pain score in the intervention stage was significantly less than that in the control stage. Conclusions: Based on the findings, environmental and behavioral interventions reduced pain and facilitated heel-prick blood sampling in preterm infants.

  4. Skin prick test in patients with chronic allergic skin disorders

    Directory of Open Access Journals (Sweden)

    Pooja Bains

    2015-01-01

    Full Text Available Background: Chronic allergic skin disorders are the inflammatory and proliferative conditions in which both genetic and environmental factors play important roles. Chronic idiopathic urticaria (CIU and atopic dermatitis (AD are among the most common chronic allergic skin disorders. These can be provoked by various food and aeroallergens. Skin prick tests (SPTs represent the cheapest and most effective method to diagnose type I hypersensitivity. Positive skin tests with a history suggestive of clinical sensitivity strongly incriminate the allergen as a contributor to the disease process. Aim s and Objectives: To determine the incidence of positive SPT in patients with chronic allergic skin disorders and to identify the various allergens implicated in positive SPT. Methods: Fifty patients of chronic allergic disorders were recruited in this study. They were evaluated by SPT with both food and aeroallergens. Results: In our study, SPT positivity in patients of CIU was 63.41% and in AD was 77.78%. Out of the 41 patients of CIU, the most common allergen groups showing SPT positivity were dust and pollen, each comprising 26.83% patients. SPT reaction was positive with food items (21.6%, insects (17.07%, fungus (12.20%, and Dermatophagoides farinae, that is, house dust mite (HDM (7.32%. The allergen which showed maximum positivity was grain dust wheat (19.51%. Among nine patients of AD, maximum SPT positivity was seen with Dermatophagoides farinae, pollen Amaranthus spinosus, grain dust wheat, and cotton mill dust; each comprising 22.22% of patients. Conclusion: Our study showed that a significant number of patients of CIU and AD showed sensitivity to dust, pollen, insects, Dermatophagoides farinae, and fungi on SPT. Thus, it is an important tool in the diagnosis of CIU and AD.

  5. The incidence and features of systemic reactions to skin prick tests.

    Science.gov (United States)

    Sellaturay, Priya; Nasser, Shuaib; Ewan, Pamela

    2015-09-01

    Skin prick testing (SPT) has been regarded as a safe procedure with few systemic reactions. To evaluate the rate of systemic reactions and their associations after SPT in the largest population to date. In this study reactions were recorded prospectively in a specialist UK allergy clinic for 6 years (2007-2013). An estimated 31,000 patients underwent SPT. Twenty-four patients (age range 7 months to 56 years, mean 23.5 years, 17 female patients, 12 with asthma) had systemic reactions. The rate of systemic reactions to SPT was 0.077%. The likely allergens causing the reaction were foods (18; peanut, 7; walnut, 1; Brazil nut, 2; pistachio, 1; lupin, 1; cow's milk, 2; shrimp, 1; spinach, 1; legume, 1; soy, 1), aeroallergens (4; rabbit, 1; rat, 1; ragwort, 1; grass pollen, 1), wasp venom (1), and Tazocin (1). The causative SPT wheal was larger than 8 mm in 75%. The reaction to Tazocin was severe, with anaphylaxis occurring minutes after SPT. Reactions were treated immediately in the clinic and did not require further medical care. In this largest single-center study, the rate of systemic reactions after SPT was 77 per 100,000 patients. It is the first study to identify foods as a common and important cause (75%), with nuts posing the highest risk. This study reports the first systemic reaction to venom SPT and the first anaphylactic reaction after drug SPT. There was an association with a history of severe reactions and large skin test reaction. There are risks, albeit small, when undertaking SPT. Copyright © 2015 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  6. Nutritive value and chemical composition of prickly pear seeds (Opuntia ficus indica L.) growing in Turkey.

    Science.gov (United States)

    Özcan, Mehmet Musa; Al Juhaimi, Fahad Y

    2011-08-01

    The proximate composition and physico-chemical properties (moisture, crude lipid, crude protein, ash, and crude fiber, peroxide value, saponification value, acidity, relative density and refractive index) of prickly pear seed and corresponding oil were determined. The mineral contents (Ca, Cu, Fe, K, Mg, Na, P, Mn and Zn) of samples were analyzed by inductively coupled plasma atomic emission spectrometry. Minerals determined were: calcium 471.2 mg/kg, potassium 532.7 mg/kg, magnesium 117.3 mg/kg, phosphorus 1,627.5 mg/kg and natrium 71.3 mg/kg. The fatty acid profiles of seed oil from the Opuntia ficus indica were analyzed by gas chromatography. Linoleic acid was established as the major fatty acid (61.01%), followed by oleic (25.52%) and palmitic (12.23%) acids. Both myristic, stearic and arachidonic acids were detected in O. ficus indica seed oil in low amounts. As a result, O. ficus indica seeds are an important source of natural fiber and, given its high linoleic acid content, its oil can be used as a nutraceutic agent.

  7. Characterization of carotenoid profile of Spanish Sanguinos and Verdal prickly pear (Opuntia ficus-indica, spp.) tissues.

    Science.gov (United States)

    Cano, M P; Gómez-Maqueo, A; García-Cayuela, T; Welti-Chanes, J

    2017-12-15

    Carotenoid profiles of different tissues (peel, pulp and whole fruit) of Spanish Sanguinos (red) and Verdal (orange) prickly pears (Opuntia ficus-indica spp.) have been characterized in detail and quantified for the first time. Carotenoids were determined by HPLC-PDA-MS (APCI + ), using a reverse phase C 30 column. A total of 9 xantophylls and 4 hydrocarbon carotenes were identified. Also, minor amounts of chlorophyll a, a' and b can be observed in Opuntia peel extracts. All carotenoids were found to be present in their free form (no carotenoid esters were detected). The RAE was highest in Opuntia peels, showing values from 19.20 to 16.48µg/100g fresh weigth, for Sanguinos and Verdal Opuntia fruits, respectively. The main carotenoid in Opuntia peel extracts was (all-E)-lutein with 1132.51 and 767.98µg/100g fresh weigth, followed by (all-E)-β-carotene with 200.40 and 173.50µg/100g fresh weigth for Sanguinos and Verdal varieties of Opuntia fruits, respectively. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Local anesthetic effects of Lidocaine cream: randomized controlled trial using a standardized prick pain.

    Science.gov (United States)

    Herberger, Katharina; Krause, Karoline; Maier, Kristina; Zschocke, Ina; Radtke, Marc; Augustin, Matthias

    2012-12-01

    ELA-max (4% lidocaine) and EMLA cream (lidocaine-prilocaine 2.5%) are topicals used for superficial anesthesia. Only few studies have been published on their comparative effectiveness in close-to-practice pain models. (1) To evaluate the analgesic efficacy of lidocaine cream compared with lidocaine-prilocaine cream and placebo. (2) To assess the safety and tolerability. Randomized, three-arm, double-blind trial in 40 healthy volunteers comparing the anesthetic effects of Lidocaine and lidocaine-prilocaine cream to placebo at various time points (0-120 min). A standardized pain was induced by lancet pricks and measured by a visual analogue scale. Intra-individual comparison between the test areas was performed in a cross-over design. Lidocaine showed significantly reduced pain compared to placebo at all assessment points. Pain reduction was achieved significantly earlier using lidocaine occlusively (30 min). No significant differences were found concerning the anesthetic efficacy of lidocaine and lidocaine-prilocaine cream. There were no relevant adverse events. This study confirms that a topical preparation with 4% lidocaine is an effective and safe treatment option for superficial anesthesia. It supports the claim that an occlusive application is more rapid in action. 4% lidocaine is of value as a rapidly-acting local anesthetic for the treatment of minor surgical procedures.

  9. Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge-Our Five Years Experience in Food Allergy Work-up.

    Science.gov (United States)

    Zivanovic, Mirjana; Atanasković-Marković, Marina; Medjo, Biljana; Gavrović-Jankulović, Marija; Smiljanić, Katarina; Tmušić, Vladimir; Djurić, Vojislav

    2017-04-01

    IgE- mediated food allergy affects 6-8% of children. Our study aimed to define the correlations between the results obtained with skin prick tests (SPTs) using commercial extracts and fresh foods, and the correlations between these result and those obtained with specific IgE (sIgE) and/ or challenge. Children aged from 2 months to 6 years were recruited prospectively. Overall 571 children were positive to one food. In all children we performed  SPT using commercial extracts of suspected food and fresh foods and sIgE. If SPT and sIgE test results did not correspond to the history, we performed open oral food challenge. Sensitivity of SPT with commercial extracts for all tested food was poor (3-35%), while  sensitivity of fresh food skin prick tests (FFSPT) was excellent (50-100%), and showed correlation with open oral food challenge (pfood extracts are more effective in detecting sensitization and with levels of sIgE greater than class 3 could predict clinical reactivity, without the need for potentially hazardous food challenges.

  10. [Localization and registration of the hinge axis in black Africans].

    Science.gov (United States)

    Assi, K D; N'Guessan, K S; N'Dindin, C; Bamba, A

    2003-06-01

    The study of the cinematic method using "SAM" and "Quick Axis of FAG" added to mandibular condyle palpation for the hinge axis limited points, show that the Black Africans mandibular condyle rotation axis position is higher (3.5 mm) and backer (2 mm) than the Caucasians. The axial points are located to between 11 and 12 mm in front of the tragus and between 7 and 8 mm below on the perpendicular line to the furrow defining the tragus superior side to the Ectocanthus.

  11. Phytochemicals, nutritionals and antioxidant properties of two prickly pear cactus cultivars (Opuntia ficus indica Mill.) growing in Taif, KSA.

    Science.gov (United States)

    Abdel-Hameed, El-Sayed S; Nagaty, Mohamed A; Salman, Mahmood S; Bazaid, Salih A

    2014-10-01

    The antioxidant properties, some phytochemicals and nutritionals were characterized in two prickly pear cactus (Opuntia ficus indica Mill.) cultivars; red and yellow; growing in Taif, Kingdom of Saudi Arabia (KSA). The antioxidant properties of red cactus cultivar were higher than the yellow cactus cultivar. Linear correlation appeared between the antioxidant properties and total phenolics. All samples nearly have the same quantity of iron, copper, sodium and potassium. Some phenolic compounds were detected by HPLC-UV analysis. HPLC-RI analysis of all samples revealed the absence of sucrose and the presence of glucose and fructose. According to the above results, this study gave a good indication about the nutritional and pharmaceutical potential of the two cactus cultivars that must be widespread cultivated in arid and semiarid regions as KSA accompanying with establishment of industries beside the cactus farms that used all parts of plants. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Facial asymmetry correction with moulded helmet therapy in infants with deformational skull base plagiocephaly.

    Science.gov (United States)

    Kreutz, Matthias; Fitze, Brigitte; Blecher, Christoph; Marcello, Augello; Simon, Ruben; Cremer, Rebecca; Zeilhofer, Hans-Florian; Kunz, Christoph; Mayr, Johannes

    2018-01-01

    The recommendation issued by the American Academy of Pediatrics in the early 1990s to position infants on their back during sleep to prevent sudden infant death syndrome (SIDS) has dramatically reduced the number of deaths due to SIDS but has also markedly increased the prevalence of positional skull deformation in infants. Deformation of the base of the skull occurs predominantly in very severe deformational plagiocephaly and is accompanied by facial asymmetry, as well as an altered ear position, called ear shift. Moulded helmet therapy has become an accepted treatment strategy for infants with deformational plagiocephaly. The aim of this study was to determine whether facial asymmetry could be corrected by moulded helmet therapy. In this retrospective, single-centre study, we analysed facial asymmetry of 71 infants with severe deformational plagiocephaly with or without deformational brachycephaly who were undergoing moulded helmet therapy between 2009 and 2013. Computer-assisted, three-dimensional, soft-tissue photographic scanning was used to record the head shape before and after moulded helmet therapy. The distance between two landmarks in the midline of the face (i.e., root of the nose and nasal septum) and the right and left tragus were measured on computer-generated indirect and objective 3D photogrammetry images. A quotient was calculated between the two right- and left-sided distances to the midline. Quotients were compared before and after moulded helmet therapy. Infants without any therapy served as a control group. The median age of the infants before onset of moulded helmet therapy was 5 months (range 3-16 months). The median duration of moulded helmet therapy was 5 months (range 1-16 months). Comparison of the pre- and post-treatment quotients of the left vs. right distances measured between the tragus and root of the nose (n = 71) and nasal septum (n = 71) revealed a significant reduction of the asymmetry (Tragus-Nasion-Line Quotient: 0

  13. Repeated touch and needle-prick stimulation in the neonatal period increases the baseline mechanical sensitivity and postinjury hypersensitivity of adult spinal sensory neurons.

    Science.gov (United States)

    van den Hoogen, Nynke J; Patijn, Jacob; Tibboel, Dick; Joosten, Bert A; Fitzgerald, Maria; Kwok, Charlie H T

    2018-03-08

    Noxious stimulation at critical stages of development has long-term consequences on somatosensory processing in later life, but it is not known whether this developmental plasticity is restricted to nociceptive pathways. Here, we investigate the effect of repeated neonatal noxious or innocuous hind paw stimulation on adult spinal dorsal horn cutaneous mechanical sensitivity. Neonatal Sprague-Dawley rats of both sexes received 4 unilateral left hind paw needle pricks (NPs, n = 13) or 4 tactile (cotton swab touch) stimuli, per day (TC, n = 11) for the first 7 days of life. Control pups were left undisturbed (n = 17). When adult (6-8 weeks), lumbar wide-dynamic-range neuron activity in laminae III-V was recorded using in vivo extracellular single-unit electrophysiology. Spike activity evoked by cutaneous dynamic tactile (brush), pinch and punctate (von Frey hair) stimulation, and plantar receptive field areas were recorded, at baseline and 2 and 5 days after left plantar hind paw incision. Baseline brush receptive fields, von Frey hair, and pinch sensitivity were significantly enhanced in adult NP and TC animals compared with undisturbed controls, although effects were greatest in NP rats. After incision, injury sensitivity of adult wide-dynamic-range neurons to both noxious and dynamic tactile hypersensitivity was significantly greater in NP animals compared with TC and undisturbed controls. We conclude that both repeated touch and needle-prick stimulation in the neonatal period can alter adult spinal sensory neuron sensitivity to both innocuous and noxious mechanical stimulation. Thus, spinal sensory circuits underlying touch and pain processing are shaped by a range of early-life somatosensory experiences.This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

  14. Probable hypoglycemic adverse drug reaction associated with prickly pear cactus, glipizide, and metformin in a patient with type 2 diabetes mellitus.

    Science.gov (United States)

    Sobieraj, Diana M; Freyer, Craig W

    2010-01-01

    To report a case of an adverse drug reaction (ADR) in a patient with type 2 diabetes mellitus taking prickly pear cactus (PPC), glipizide, and metformin. A 58-year-old Mexican male with type 2 diabetes mellitus being treated with metformin 1000 mg twice daily and extended-release glipizide 10 mg daily was referred to the pharmacist for medication education. He denied taking herbal supplements or experiencing hypoglycemia. Two hemoglobin A(1c) values (6.8% and 6.7%) obtained over the past year demonstrated glycemic control, which was supported by his reported fasting blood glucose readings of 113-132 mg/dL. One month later, the patient reported 4 hypoglycemic events with blood glucose readings of 49-68 mg/dL, which resulted in discontinuation of glipizide. One month later, the patient denied any further hypoglycemia. During medication reconciliation he reported consuming crude PPC pads daily for 2 months for glucose control. Literature suggests that PPC has an effect on lowering blood glucose levels in patients with type 2 diabetes mellitus, although few identified data describe ADRs from combining PPC with other agents used in treating type 2 diabetes mellitus. A literature search of MEDLINE (through December 2009) using the search terms diabetes mellitus, prickly pear cactus, nopal, opuntia, metformin, glipizide, glyburide, glimepiride, and sulfonylurea revealed no case reports of the described ADR. One case report describing the blood glucose-lowering effect of PPC in a patient concurrently taking oral antihyperglycemics documented an episode of hypoglycemia, although the Naranjo probability scale was not applied. One patient survey discovered the most common drug-herbal interaction in the given population to be between PPC and antihyperglycemic agents, resulting in hypoglycemia. In our case, use of the Naranjo probability scale suggests the ADR to be probable. The mechanism may be due to the additive glucose lowering of the 3 agents consumed concurrently by the

  15. IgE Sensitization Patterns to Commonly Consumed Foods Determined by Skin Prick Test in Korean Adults.

    Science.gov (United States)

    Kim, Sung Ryeol; Park, Hye Jung; Park, Kyung Hee; Lee, Jae-Hyun; Park, Jung-Won

    2016-08-01

    Offending food allergens can vary with regional preferences in food consumption. In this study, we analysed sensitization rates to commonly consumed foods in Korean adults suspected of having food allergy. One hundred and thirty four subjects underwent a skin prick test (SPT) with 55 food allergens, of which 13 were made by our laboratory and the rest were commercially purchased. Of the 134 patients, 73 (54.5%) were sensitized to one or more food allergens. Sensitization to chrysalis was detected most frequently, at a rate of 25.4%. Sensitization rates to other food allergens were as follows: maize grain (13.4%), shrimp (11.9%), almond (11.1%), wheat flour (8.2%), lobster (8.2%), buckwheat (8.2%), mackerel (5.2%), pollack (5.2%), halibut (4.5%), peanut (4.5%), anchovy (4.4%), squid (3.7%), saury (3.0%), common eel (3.0%), yellow corvina (3.0%), hairtail (2.2%), octopus (2.2%), and others. In addition to well-known food allergens, sensitivity to mackerel, chrysalis, pollack, and halibut, which are popular foods in Korea, was observed at high rates in Korean adults. We suggest that the SPT panel for food allergy in Korea should include these allergens.

  16. IgE Sensitization Patterns to Commonly Consumed Foods Determined by Skin Prick Test in Korean Adults

    Science.gov (United States)

    2016-01-01

    Offending food allergens can vary with regional preferences in food consumption. In this study, we analysed sensitization rates to commonly consumed foods in Korean adults suspected of having food allergy. One hundred and thirty four subjects underwent a skin prick test (SPT) with 55 food allergens, of which 13 were made by our laboratory and the rest were commercially purchased. Of the 134 patients, 73 (54.5%) were sensitized to one or more food allergens. Sensitization to chrysalis was detected most frequently, at a rate of 25.4%. Sensitization rates to other food allergens were as follows: maize grain (13.4%), shrimp (11.9%), almond (11.1%), wheat flour (8.2%), lobster (8.2%), buckwheat (8.2%), mackerel (5.2%), pollack (5.2%), halibut (4.5%), peanut (4.5%), anchovy (4.4%), squid (3.7%), saury (3.0%), common eel (3.0%), yellow corvina (3.0%), hairtail (2.2%), octopus (2.2%), and others. In addition to well-known food allergens, sensitivity to mackerel, chrysalis, pollack, and halibut, which are popular foods in Korea, was observed at high rates in Korean adults. We suggest that the SPT panel for food allergy in Korea should include these allergens. PMID:27478328

  17. A tale of ENSO, PDO, and increasing aridity impacts on drought-deciduous shrubs in the Death Valley region.

    Science.gov (United States)

    Ehleringer, James R; Sandquist, Darren R

    2018-06-28

    Germination, establishment, phenology, and death among three drought-deciduous shrubs were influenced by ENSO/PDO and precipitation, based on 37 years of annual surveys. Encelia farinosa forms near monospecific stands on slopes, whereas E. frutescens and Ambrosia salsola dominate wash habitats. All shrubs exhibited phenological coherence. While germination, establishment, and mortality patterns were similar among wash species, these dynamics contrasted with E. farinosa on slopes. Germination was associated with El Niño years. Slope plant establishment was dependent on precipitation in the subsequent year, but not evidently so in wash species. Major mortality events were episodic, with Encelia mortality just as likely to occur in years with below or above average precipitation. In both Encelia species, mortality was associated with transitions to a cold PDO phase. In E. frutescens this response was more rapid, whereas in E. farinosa mortality lagged 1 year, resulting in contrasting slope-wash mortality patterns. 50% of newly established shrubs died within 5, 5, and 18 years for E. frutescens, E. farinosa, and A. salsola, respectively. The 90% mortality ages were 26 years for E. frutescens, 24 years for E. farinosa, and 51 years for A. salsola. While maximum life expectancies are unknown, estimated maximum life expectancies were 56, 66, and 86 years for E. frutescens, E. farinosa, and A. salsola, respectively. Overall, as the climate has become more arid over the past four decades, the populations in both slope and wash habitats have exhibited similar responses: reduced shrub abundances and reduced total supportable leaf areas.

  18. Skin prick test results in patients with atopic symptoms in Yozgat district

    Directory of Open Access Journals (Sweden)

    Emine Çölgeçen

    2014-03-01

    Full Text Available Objective: Skin prick test (SPT is used widely and fastly to determine the allergens in atopic disease. We aimed in this study to analysis the relationship between total serum IgE (T.IgE and allergens that found by SPT and looking over the frequency of allergens. Methods: We evaluated clinical findings and SPT results and T.IgE levels of 190 patients who admitted to dermatology outpatient clinic, retrospectively. Rsults: 53 (27.9% patients had idiopathic generalized pruritus (IGP, 41 (21.6% had atopic dermatitis (AD, 40 (21.1% had chronic idiopathic urticaria (CIU, 38 (20% had allergic rhinitis (AR, and 18 (9.5% had allergic asthma (AA. 123 (64.7% patients had positive reaction to at least one or more allergen on SPT. Of these patients 28 were IGP, 30 were AD, 20 were CIU, 28 were AR, and 17 were AA. The most common allergens were pine pollen (25.3%, wheat pollen (18.4%, and dog epithelium (15.8%. The mean levels of T.IgE was 382.61 ± 517.28 IU/ml (1-2500. T.IgE is higher 54.9% of patients. It was seen that DPT positivity was significantly more frequent in patients with increased T.IgE levels than those with normal total serum IgE level (p<0.05. Conclusion: We think that pine tree and wheat pollens are the most commonly encountered allergens at Yozgat area. Moreover, our findings indicated to the requirement and benefits of DPT in patients with AD, AR and AA in addition to the those KİU and İJP, particularly in patients with increased T.IgE levels, although further studies with larger sample size are needed. J Clin Exp Invest 2014; 5 (1: 64-68

  19. Prickly pear cactus (Opuntia ficus indica var. saboten) protects against stress-induced acute gastric lesions in rats.

    Science.gov (United States)

    Kim, Seung Hyun; Jeon, Byung Ju; Kim, Dae Hyun; Kim, Tae Il; Lee, Hee Kyoung; Han, Dae Seob; Lee, Jong-Hwan; Kim, Tae Bum; Kim, Jung Wha; Sung, Sang Hyun

    2012-11-01

    The protective activity of prickly pear cactus (Opuntia ficus indica var. saboten) fruit juice and its main constituent, betanin, were evaluated against stress-induced acute gastric lesions in rats. After 6 h of water immersion restraint stress (WIRS), gastric mucosal lesions with bleeding were induced in Sprague-Dawley rats. Pretreatment of a lyophilized powder containing O. ficus indica var. saboten fruit juice and maltodextrin (OFSM) and betanin significantly reduced stress lesions (800-1600 mg/kg). Both OFSM and betanin effectively prevented the decrease in gastric mucus content as detected by alcian blue staining. In addition, OFSM significantly suppressed WIRS-induced increases in the level of gastric mucosal tumor necrosis factor-α and myeloperoxidase (MPO). Betanin alone was only effective in decreasing MPO. These results revealed the protective activity of OFSM against stress-induced acute gastric lesions and that betanin may contribute to OFSM's gastric protective activity, at least in part. When OFSM and betanin were taken together, OFSM exerted gastroprotective activity against stress-induced gastric lesions by maintaining gastric mucus, which might be related to the attenuation of MPO-mediated damage and proinflammatory cytokine production.

  20. Pectin isolated from prickly pear (Opuntia SSP) modifies LDL metabolism in cholesterol-fed guinea pigs

    International Nuclear Information System (INIS)

    Fernandez, M.L.; McNamara, D.J.

    1990-01-01

    The effects of dietary pectin on plasma and hepatic cholesterol (CH) levels, plasma lipoprotein profiles, hepatic 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) reductase activity, and low density lipoprotein (LDL) binding to hepatic membranes were investigated by feeding 1% pectin to guinea pigs on a high CH diet. Animals were fed either chow + 0.25% CH (HC diet) or the CH diet + 1% prickly pear pectin (HC-P diet) for 25 days. Plasma CH levels were decreased 26% by the HC-P with 33% decreases in LDL and KDL. LDL peak density shifted from 1.040 to 1.055 g/ml with pectin. Hepatic total, free and esterified CH levels were reduced 60, 40 and 85% respectively by the HC-P diet. In contrast, HMG-CoA reductase activity was unaffected. 125 I-LDL binding to hepatic membranes was increased by intake of the HC-P diet compared to the HC diet. The affinity of the apo B/E receptor for LDL was not affected by dietary pectin while the receptor number was increased 1.5-fold in animals on the HC-P diet. These data suggest that the parameters of HC metabolism affected by dietary pectin are consistent with an increased demand on the hepatic CH pools which possibly results from increased fecal excretion of bile acids

  1. The position of the occlusal plane in natural and artificial dentitions as related to other craniofacial planes.

    Science.gov (United States)

    Al Quran, Firas A M; Hazza'a, Abdalla; Al Nahass, Nabeel

    2010-12-01

    This study aimed at determining the most reliable ala-tragus line as a guide for the orientation of the occlusal plane in complete denture patients by use of cephalometric landmarks on dentate volunteers. Analysis was made for prosthodontically related craniofacial reference lines and angles of lateral cephalometric radiographs taken for 47 dentate adults. Variables were determined and data were analyzed using SPSS (SPSS, Inc., Chicago, IL). Occlusal plane angle formed between the occlusal plane and Camper's plane had the lowest mean value in the angle formed with Camper's I, which represents the measure taken from the superior border of the tragus of the ear with a score of 2.1°. The highest was measured in the angle formed with Camper's III with a score of 6.1°, while the angle formed with Camper's II was 3.2°. The differences between the three planes in relation to the occlusal plane was significant (p < 0.001). The superior border of the tragus with the inferior border of the ala of the nose was most accurate in orienting the occlusal plane. © 2010 by The American College of Prosthodontists.

  2. Armazenamento de figo-da-índia em pó Storage of the prickly pear powder

    Directory of Open Access Journals (Sweden)

    Cícera G. C. de Lisbôa

    2012-02-01

    Full Text Available Neste trabalho foram avaliadas a estabilidade do figo-da-índia em pó acondicionado em embalagens laminadas durante 100 dias de armazenamento, sob condições controladas de temperatura e umidade relativa e as amostras a cada 20 dias, por meio do teor de água, atividade de água, acidez total titulável, açúcares redutores e cor (luminosidade, intensidade de vermelho e de amarelo. Observou-se que as embalagens laminadas não evitaram a absorção de água, enquanto a atividade de água aumentou e os açúcares redutores diminuíram com o tempo; por outro lado, a acidez total titulável diminuiu mas apenas nos primeiros vinte dias; enfim, todas as amostras sofreram escurecimento durante o armazenamento.The stability of the prickly pear power packed in laminated roll for 100 days storage under controlled temperature and relative humidity was evaluated. The material was evaluated every 20 days, through the moisture content, water activity, total acidity, sugars and color (brightness, red and yellow colour intensity. It was found during storage of powder samples, that the laminated packaging did not prevent the water absorption, while water activity increased and reduced sugars decreased with time, the total acidity decreased in the first twenty days, keeping stable thereafter in all samples and there was a darkening of the powder samples stored at 40 ºC.

  3. Nutritive value of some herbages for dromedary camel in Iran.

    Science.gov (United States)

    Towhidi, A

    2007-01-01

    To prepare standard tables of chemical composition of feedstuffs and to determine digestibility and palatability of different plant species in dromedary camel, this research was carried out by considering the most consuming herbages of Iranian desert rages. The plant species were included Atriplex lentiformis, Alhagi persarum, Seidlitzia rosmarinus, Saueda fruticosa, Haloxylon ammodendron, Tamarix kotschyi, Hammada salicornica, Salsola yazdiana, Salsola tomentosa, Tamarix aphylla and Artemisia sieberi. Thirty samples of the browsing parts were collected from the rangelands of Yazd province in autumn. Chemical composition of samples including Dry Matter (DM), Crude Protein (CP), Crude Fiber (CF), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Ether Extract (EE), Total Ash (TA), macro elements (Ca, P, Mg, K), micro elements (Fe, Mg, Cu, Zn)and gross energy (GE) were analyzed. The in vitro digestibility was determined by camel rumen liquor in Tilley and Terry method. Palatability of the plants were measured by three mature camels in cafeteria trials. The camels voluntarily fed 11 plant species during one hour for six days. Data were analyzed by GLM method in SAS software. The highest CP (18.3%) and the lowest NDF (40.4%) and ADF (35.4%) were related to Tamarix aphylla. The lowest CP (5.5%) and the highest NDF (72.8%) and ADF (59.6%) were related to Artemisia sieberi. The highest organic matter digestibility in dry matter was related to Haloxylon ammodendron. The results also indicated that Atriplex lentiformis, Alhagi persarum, Seidlitzia rosmarinus, Saueda fruticosa, Haloxylon ammodendron, Salsola tomentosa, Hammada salicornica, Tamarix kotschyi, Salsola yazdiana, Tamarix aphylla and Artemisia sieberi were more pleasure feed, respectively. It was not observed any correlation between %DOMD and chemical composition. Moreover, There was not a consistent relationship between the palatability of herbages with %DOMD or chemical composition.

  4. Analysis of Relative Parallelism Between Hamular-Incisive-Papilla Plane and Campers Plane in Edentulous Subjects: A Comparative Study.

    Science.gov (United States)

    Tambake, Deepti; Shetty, Shilpa; Satish Babu, C L; Fulari, Sangamesh G

    2014-12-01

    The study was undertaken to evaluate the parallelism between hamular-incisive-papilla plane (HIP) and the Campers plane. And to determine which part of the posterior reference of the tragus i.e., the superior, middle or the inferior of the Camper's plane is parallel to HIP using digital lateral cephalograms. Fifty edentulous subjects with well formed ridges were selected for the study. The master casts were obtained using the standard selective pressure impression procedure. On the deepest point of the hamular notches and the centre of the incisive papilla stainless steel spherical bearings were glued to the cast at the marked points. The study templates were fabricated with autopolymerizing acrylic resin. The subjects were prepared for the lateral cephalograms. Stainless steel spherical bearings were adhered to the superior, middle, inferior points of the tragus of the ear and inferior border of the ala of the nose using surgical adhesive tape. The subjects with study templates were subjected to lateral cephalograms. Cephalometric tracings were done using Autocad 2010 software. Lines were drawn connecting the incisive papilla and hamular notch and the stainless steel spherical bearings placed on the superior, middle and inferior points on the tragus and the ala of the nose i.e., the Campers line S, Campers line M, Campers line I. The angles between the three Camper's line and the HIP were measured and recorded. Higher mean angulation was recorded in Campers line S -HIP (8.03) followed by Campers line M-HIP (4.60). Campers line I-HIP recorded the least angulation (3.80). The HIP is parallel to the Camper's plane. The Camper's plane formed with the posterior reference point as inferior point of the tragus is relatively parallel to the HIP.

  5. Protective effect of Opuntia ficus indica f. inermis prickly pear juice upon ethanol-induced damages in rat erythrocytes.

    Science.gov (United States)

    Alimi, Hichem; Hfaeidh, Najla; Bouoni, Zouhour; Sakly, Mohsen; Ben Rhouma, Khémais

    2012-05-01

    Juice from the fruit of the cactus Opuntia ficus indica is claimed to possess several health-beneficial properties. The present study was carried out to determine whether O. ficus indica f. inermis fruit extract might have a protective effect upon physiological and morphological damages inflicted to erythrocytes membrane by chronic ethanol poisoning, per os, in rat. Chemical analysis of the extract revealed the presence of polyphenols, flavonoids, ascorbic acid, carotenoids, and betalains. Ethanol administration (3 g/kg b.w, per day for 90 days) induced an increase of malondialdehyde (MDA) and carbonylated proteins levels and a decrease of glutathione (GSH) level in erythrocyte. Ethanol administration also reduced the scavenging activity in plasma and enhanced erythrocytes hemolysis, as compared to control rats. In addition, ethanol intake increased the erythrocyte shape index by +895.5% and decreased the erythrocyte diameter by -61.53% as compared to controls. In animals also given prickly pear juice during the same experimental period, the studied parameters were much less shifted. This protective effect was found to be dose-dependent. It is likely that the beneficial effect of the extract is due to the high content of antioxidant compounds. Copyright © 2012 Elsevier Inc. All rights reserved.

  6. In Vitro Propagation of Three Moroccan Prickly Pear Cactus Opuntia and Plant Establishment in Soil

    Directory of Open Access Journals (Sweden)

    Aissam EL FINTI

    2013-02-01

    Full Text Available Opuntia is one of the most widespread cacti, primarily due to their edible fruit and vegetable mass used as feed. The high demand for young plants of Opuntia made it necessary to find a rapid method of multiplication of the cactus, the safest method consisting in vitro micropropagation of species belonging to this genus. With aim of large production of plant material, a propagation system of three important prickly pear cactus cultivar (Opuntia ficus-indica in Morocco was developed. Segments of healthy young cladode (containing one areole were cultivated in Murashige and Skoog medium (MS containing adenine sulfate (40 mg/1, monosodium phosphate (50 mg/l, sucrose (50 g/l, phytagel (0.3% and benzyladenine (BA at 22.2 μM, to start the process of micropropagation. In vitro-developed shoots from areoles were used as secondary explants to induce shoot development in the MS medium with 5 mg/l of BA. All of the three studied cultivars showed an important multiplication rate in this medium. ‘Sidi Ifni M’ (‘Moussa’ cultivar shows the greatest number of shoots followed by ‘Sidi Ifni A’ (‘Aissa’ and ‘Delahia’ 17.26, 14.12 and 12.13 respectively. Rooting of in vitro-generated shoots was achieved most efficiently on half-strength MS basal medium supplemented with 0.5 mg/l of indole-3-butyric acid (IBA or IAA. Rooting frequencies were in the range from 95 to 100% and the highest mean number of root (19.1 was obtained with IBA for ‘Delahia’ cultivar. All micropropagated plants were transferred to greenhouse and all of them survived acclimatization process and showed good overall growth.

  7. The effect of soil environment on germination and emergence of prickly lettuce (Lactuca serriola L.

    Directory of Open Access Journals (Sweden)

    Jan Kapeluszny

    2012-12-01

    Full Text Available In 2007 mature achenes of prickly lettuce (Lactuca serriola L. were collected from a winter wheat crop growing on loess-derived brown earth soil, from the side of a dirt road, and from a hybrid alfalfa crop growing on heavy rendzina soil. In March 2008 the germination ability of achenes and the length of roots and above-ground parts of L. serriola seedlings were determined. The experiment was set up as a completely randomized design in 6 replicates and it was done in dishes. The dishes were filled with loess-derived brown soil and podzolic soil derived from light loamy sand with different pH values of 4.5, 5.5 and 7.0 obtained using laboratory methods. The second experiment evaluated the effect of medium type and light conditions on the germination of Lactuca serriola achenes. The experimental design was the same as in the above described experiment. The study found that there were significantly more germinating seeds from the plants growing in the alfalfa crop and on the dirt roadside compared to the plants from the wheat crop. Emerging plants showed higher germination ability on loess-derived soil with a pH of 5.5 and 7.0 than on podzolic soil derived from sand with the same pH. Lactuca serriola prefers soils rich in calcium compounds, and developing segetal cenopopulations produce diaspores capable of germination, thereby the numbers of individuals can increase and the continuity of this species in crop fields can be maintained. Seeds germinated significantly better in the dark than under the conditions of 12-hour illumination.

  8. Characterisation of Fractures and Fracture Zones in a Carbonate Aquifer Using Electrical Resistivity Tomography and Pricking Probe Methodes

    Science.gov (United States)

    Szalai, Sandor; Kovacs, Attila; Kuslits, Lukács; Facsko, Gabor; Gribovszki, Katalin; Kalmar, Janos; Szarka, Laszlo

    2018-04-01

    Position, width and fragmentation level of fracture zones and position, significance and characteristic distance of fractures were aimed to determine in a carbonate aquifer. These are fundamental parameters, e.g. in hydrogeological modelling of aquifers, due to their role in subsurface water movements. The description of small scale fracture systems is however a challenging task. In the test area (Kádárta, Bakony Mts, Hungary), two methods proved to be applicable to get reasonable information about the fractures: Electrical Resistivity Tomography (ERT) and Pricking-Probe (PriP). PriP is a simple mechanical tool which has been successfully applied in archaeological investigations. ERT results demonstrated its applicability in this small scale fracture study. PriP proved to be a good verification tool both for fracture zone mapping and detecting fractures, but in certain areas, it produced different results than the ERT. The applicability of this method has therefore to be tested yet, although its problems most probably origin from human activity which reorganises the near-surface debris distribution. In the test site, both methods displayed fracture zones including a very characteristic one and a number of individual fractures and determined their characteristic distance and significance. Both methods prove to be able to produce hydrogeologically important parameters even individually, but their simultaneous application is recommended to decrease the possible discrepancies.

  9. House dust bioactivities predict skin prick test reactivity for children with high risk of allergy.

    Science.gov (United States)

    Kim, Haejin; Tse, Kevin; Levin, Linda; Bernstein, David; Reponen, Tiina; LeMasters, Grace; Lummus, Zana; Horner, Anthony A

    2012-06-01

    Although evidence suggests that ambient exposures to endotoxin and other immunostimulants during early life influence allergic risk, efforts to understand this host-environment relationship have been hampered by a paucity of relevant assays. These investigations determined whether parameters of house dust extract (HDE) bioactivity were predictive of allergen skin prick test (SPT) reactivity for infants at high risk of allergy participating in the Cincinnati Childhood Allergy and Air Pollution Study (CCAAPS). We conducted a nested case-control study, selecting 99 CCAAPS children who had positive SPT results to at least 1 aeroallergen at age 3 years and 101 subjects with negative SPT results. HDEs were prepared from dust samples collected from the subjects' homes at age 1 year. Murine splenocytes and bone marrow-derived dendritic cells were incubated with HDEs, and supernatant cytokine concentrations were determined by means of ELISA. Alternatively, bone marrow-derived dendritic cells were preincubated with HDEs, and then LPS-induced IL-6 responses were assessed. HDE endotoxin levels were determined by using the limulus amebocyte lysate assay. HDEs derived from the homes of children with positive (cases) and negative (control subjects) SPT results had similar bioactivities. However, when cases were considered in isolation, HDEs with higher levels of bioactivity were significantly associated with children who had lower numbers of positive SPT results. Analogous statistical analyses did not identify any association between HDE endotoxin levels and the aeroallergen sensitization profiles of children included in this study. HDE immunostimulatory activities predicted the aeroallergen sensitization status of CCAAPS subjects better than HDE endotoxin levels. These results provide the first published evidence that HDE bioassays have clinical relevance in predicting atopic risk. Copyright © 2012 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All

  10. Medical clowns ease anxiety and pain perceived by children undergoing allergy prick skin tests.

    Science.gov (United States)

    Goldberg, A; Stauber, T; Peleg, O; Hanuka, P; Eshayek, L; Confino-Cohen, R

    2014-10-01

    Intervention of medical clowns (MC) during various medical procedures performed in children has been used to relieve anxiety and pain. Their role in allergy skin testing has never been evaluated. To evaluate whether MC can diminish pain and anxiety perceived by children undergoing allergy skin prick tests (SPT). In a prospective, randomized, controlled, and blinded study, children undergoing SPT were or were not accompanied by MC. All parents and children ≥8 years completed the State-Trait Anxiety Inventory (STAI) before and after SPT. Videotapes recorded during the procedure were scored for anxiety (m-YPAS) for all children and for pain (FLACC) for children 2-7 years old by a psychologist who was unaware of the MC's presence. After SPT, children ≥8 years completed a visual analog score (VAS) for pain. Ninety-one children (mean age 8.2 years, M/F = 54/37) were recruited of whom 45 were accompanied by clowns. A significant reduction in state-STAI was found in the clowns group, in both parents and children, when compared with the regular group (26.9 ± 6.6 and 32.3 ± 10.0; P = 0.004, and 27.1 ± 4.2 and 34.3 ± 7.6; P = 0.002, respectively). Both m-YPAS and FLACC were reduced in the clowns group compared with the regular one. In the clowns group, m-YPAS positively correlated with both VAS and FLACC (P = 0.000 and 0.002, respectively). m-YPAS was positively correlated with FLACC in the regular group (P = 0.000). Medical clowns significantly decrease the level of anxiety perceived by both children undergoing allergy SPT and their parents, as well as the pain perceived by young children. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP . BY SOLID STATE FERMENTATION OF PRICKLY PEAR

    Directory of Open Access Journals (Sweden)

    TAMIRES CARVALHO DOS SANTOS

    2016-01-01

    Full Text Available Prickly palm cactus husk was used as a solid - state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box - Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp . Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

  12. Evaluation of the frequency of food allergens based on skin prick test in children in Kurdistan Province - Iran.

    Science.gov (United States)

    Kalmarzi, R; Ataee, P; Homagostar, Gh; Tagik, M; Ghaderi, E; Kooti, W

    Food allergy refers to abnormal reactions of the body caused by an immune system response to food. This study was conducted aiming to investigate allergy to food allergens in children with food allergies. This study was conducted as a cross-sectional one on 304 children aged six months to seven years with food allergies admitted to the tertiary referral hospital in Kurdistan Province - Iran, during 2014-2015. All the patients were examined for skin prick test using 49 allergens. Finally, the obtained data were analysed using SPSS15 and chi-square and t tests. The highest percentage of occurrence of bump reaction (wheal) and redness (flare) was due to the consumption of fish, eggs, tomatoes, and cocoa. Moreover, the lowest rate of wheal and flare was caused by exposure to allergens like latex, tea, malt, and wheat flour. The reaction most created due to the consumption of foods was flare which was higher among under three-year-olds group (p<0.05), and between the sexes, girls showed the most common allergic reactions (p<0.05). Since food allergy has a high prevalence in children, it should be considered with great interest. Considering that avoiding food allergens is the first step in the treatment of food allergies, the present study may be a useful guide in this regard. Copyright © 2017 SEICAP. Published by Elsevier España, S.L.U. All rights reserved.

  13. The Relationship of the Facial Nerve to the Condylar Process: A Cadaveric Study with Implications for Open Reduction Internal Fixation

    Directory of Open Access Journals (Sweden)

    H. P. Barham

    2015-01-01

    Full Text Available Introduction. The mandibular condyle is the most common site of mandibular fracture. Surgical treatment of condylar fractures by open reduction and internal fixation (ORIF demands direct visualization of the fracture. This project aimed to investigate the anatomic relationship of the tragus to the facial nerve and condylar process. Materials and Methods. Twelve fresh hemicadavers heads were used. An extended retromandibular/preauricular approach was utilized, with the incision being based parallel to the posterior edge of the ramus. Measurements were obtained from the tragus to the facial nerve and condylar process. Results. The temporozygomatic division of the facial nerve was encountered during each approach, crossing the mandible at the condylar neck. The mean tissue depth separating the facial nerve from the condylar neck was 5.5 mm (range: 3.5 mm–7 mm, SD 1.2 mm. The upper division of the facial nerve crossed the posterior border of the condylar process on average 2.31 cm (SD 0.10 cm anterior to the tragus. Conclusions. This study suggests that the temporozygomatic division of the facial nerve will be encountered in most approaches to the condylar process. As visualization of the relationship of the facial nerve to condyle is often limited, recognition that, on average, 5.5 mm of tissue separates condylar process from nerve should help reduce the incidence of facial nerve injury during this procedure.

  14. Impact of grazing on range plant community components under arid Mediterranean climate in northern Syria

    NARCIS (Netherlands)

    Niane, A.A.

    2013-01-01

    Keywords: Rotational grazing, full protection, continuous grazing species richness,

    species diversity, soil seed bank, Bayesian methods, Salsola vermiculata, seed

    longevity, rangeland management, Syria.

    Rangelands represent 70% of the semi-arid and arid

  15. Fe, Cr, Ni, Cu, Mg, Al, Ti, and S contents in plants and soil of heaps of nickel smelting works

    Energy Technology Data Exchange (ETDEWEB)

    Banasova, V; Hajduk, J

    1977-01-01

    The writers established the Fe, Ce, Cr, Ni, Ca, Mg, Al, Ti and S contents in the neopedon of heaps piling up from processing of nickel ore as well as in the plants: Cardaria draba, Salsola cali, Agropyrum repens, Bromus erectus, Calamagrostis epigeios, Cynodon dactylon and Matricaria inodora, growing on such heaps. Ca, Mg and S contents were found to be higher in dicotyledons and Fe, Al, Ti, Ni and Cr contents higher in monocotyledons. The analyzed dicotyledons appeared to be concentrators of Ca and S. Highest Fe, Al, Ti, Ni and Cr contents were found in individuals of the species Agropyrum repens. The neopedon as well as the plants had extraordinarily high Cr concentrations. The species Salsola cali has been found to possess an unusually higher affinity to the dump substrate after processing of nickel ore and to be a concentrator of Mg. 16 references, 1 table.

  16. New additions to the Flora of Ethiopia and Eritrea in the families Euphorbiaceae, Fabaceae (Leguminosae), Lamiaceae, Campanulaceae, Eriocaulaceae and Poaceae

    DEFF Research Database (Denmark)

    Friis, Ib; Phillips, Sylvia M.; Gilbert, Michael G.

    2011-01-01

    During recent field work by Ib Friis and Sally Bidgood six collections were collected that did not represent taxa accounted for in the Flora of Ethiopia and Eritrea. These were Phyllanthus chevalieri, Indigofer bracteolata, Wahlenbergia paludicola, Clerodendrum triflorum, Tragus mongolorum and Hy...

  17. Using a network modularity analysis to inform management of a rare endemic plant in the northern Great Plains, USA

    Science.gov (United States)

    Larson, Diane L.; Droege, Sam; Rabie, Paul A.; Larson, Jennifer L.; Devalez, Jelle; Haar, Milton; McDermott-Kubeczko, Margaret

    2014-01-01

    1. Analyses of flower-visitor interaction networks allow application of community-level information to conservation problems, but management recommendations that ensue from such analyses are not well characterized. Results of modularity analyses, which detect groups of species (modules) that interact more with each other than with species outside their module, may be particularly applicable to management concerns. 2. We conducted modularity analyses of networks surrounding a rare endemic annual plant, Eriogonum visheri, at Badlands National Park, USA, in 2010 and 2011. Plant species visited were determined by pollen on insect bodies and by flower species upon which insects were captured. Roles within modules (network hub, module hub, connector and peripheral, in decreasing order of network structural importance) were determined for each species. 3. Relationships demonstrated by the modularity analysis, in concert with knowledge of pollen species carried by insects, allowed us to infer effects of two invasive species on E. visheri. Sharing a module increased risk of interspecific pollen transfer to E. visheri. Control of invasive Salsola tragus, which shared a module with E. visheri, is therefore a prudent management objective, but lack of control of invasive Melilotus officinalis, which occupied a different module, is unlikely to negatively affect pollination of E. visheri. Eriogonum pauciflorum may occupy a key position in this network, supporting insects from the E. visheri module when E. visheri is less abundant. 4. Year-to-year variation in species' roles suggests management decisions must be based on observations over several years. Information on pollen deposition on stigmas would greatly strengthen inferences made from the modularity analysis. 5. Synthesis and applications: Assessing the consequences of pollination, whether at the community or individual level, is inherently time-consuming. A trade-off exists: rather than an estimate of fitness effects, the

  18. Sensitization to cereals and peanut evidenced by skin prick test and specific IgE in food-tolerant, grass pollen allergic patients

    Directory of Open Access Journals (Sweden)

    Martens Maria

    2011-12-01

    Full Text Available Abstract Background The botanical relation between grass and cereal grains may be relevant when diagnosing food allergy to cereals. The aim was to investigate the diagnostic specificity of skin prick test (SPT and specific immunoglobulin E (sIgE tests to cereals and peanut in grass pollen allergic subjects without history of, and clinically reactions to foods botanically related to grass. Methods 70 subjects (41 females; mean age 32 years and 20 healthy controls (13 females; mean age 24 years were tested by open food challenge (OFC with cereals and peanut. SPT and sIgE both with Immulite® (Siemens and ImmunoCAP® (Phadia to grass and birch pollen, cereals, peanut and bromelain were performed. Results Of the 65 OFC-negative subjects 29-46% (SPT, depending on cut-off, 20% (Immulite and 38% (ImmunoCAP had positive results to one or more of the foods tested. Controls were negative in all tests. Cross-reactive carbohydrate determinants (CCD as evidenced by reaction to bromelain could explain only a minority of the measured IgE-sensitizations. Conclusion Grass pollen allergic patients with documented food tolerance to cereals and peanut may express significant sensitization. False-positive cereal or peanut allergy diagnoses may be a quantitatively important problem both in routine clinical work and epidemiological studies.

  19. Head posture and neck pain of chronic nontraumatic origin: a comparison between patients and pain-free persons.

    Science.gov (United States)

    Silva, Anabela G; Punt, T David; Sharples, Paul; Vilas-Boas, João P; Johnson, Mark I

    2009-04-01

    To compare standing head posture measurements between patients with nontraumatic neck pain (NP) and pain-free individuals. Single-blind (assessor) cross-sectional study. Hospital and general community. Consecutive patients (n=40) with chronic nontraumatic NP and age- and sex-matched pain-free participants (n=40). Not applicable. Three angular measurements: the angle between C7, the tragus of the ear, and the horizontal; the angle between the tragus of the ear, the eye, and the horizontal; and the angle between the inferior margins of the right and the left ear and the horizontal were calculated through the digitization of video images. NP patients were found to have a significantly smaller angle between C7, the tragus, and the horizontal, resulting in a more forward head posture than pain-free participants (NP, mean +/- SD, 45.4 degrees +/-6.8 degrees ; pain-free, mean +/- SD, 48.6 degrees +/-7.1 degrees ; P50y) revealed an interaction, with a statistically significant difference in head posture for younger participants with NP compared with younger pain-free participants (NP, mean +/- SD, 46.1 degrees +/-6.7 degrees ; pain-free, mean +/- SD, 51.8 degrees +/-5.9 degrees ; P.05; CI for the difference between groups, -4.9 degrees -4.2 degrees ). No other differences were found between patients and pain-free participants. Younger patients with chronic nontraumatic NP were shown to have a more forward head posture in standing than matched pain-free participants. However, the difference, although statistically significant, was perhaps too small to be clinically meaningful.

  20. Preparation and characterization of molecularly-imprinted polymers for extraction of sanshool acid amide compounds followed by their separation from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum).

    Science.gov (United States)

    Chen, Xiaolong; Jin, Xinkai; Li, Yao; Chen, Guangjing; Chen, Kewei; Kan, Jianquan

    2018-01-01

    Molecularly imprinted polymers were prepared using the molecular structure analogs of sanshool as template molecule, 2-vinylpyridine and β-cyclodextrin as double functional monomers, ethylene dimethacrylate as cross linker, and azobisisobutyronitrile as initiator. The structural characteristics of the polymers were determined by Fourier-transform infrared spectroscopy and scanning electron microscopy. Dynamic adsorption and isothermal adsorption were also investigated. The molecularly imprinted polymers were used to prepare a molecularly imprinted solid-phase extraction column in order to separate acid amide components from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum). After eluting, the percentage of acid amide components was enhanced to 92.40 ± 1.41% compared with 23.34 ± 1.21% in the initial pepper oil resin, indicating good properties of purification of molecularly imprinted polymers and potential industrial application. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Apport de l'approche systémique des systèmes d'élevage à l ...

    African Journals Online (AJOL)

    Le paysage est occupé par de nouvelles steppes issues de dégradation où dominent Salsola vermiculata, Atractylis serratuloides, Noaea mucronata, Peganum harmala. Les formations psammophytes à Retama raetam, Thymelaea microphylla et Tamarix africana, s'étendent sur plus de 17 %. L'ensablement menace ces ...

  2. Relevance of Cat and Dog Sensitization by Skin Prick Testing in Childhood Eczema and Asthma.

    Science.gov (United States)

    Hon, Kam Lun; Tsang, Kathy Yin Ching; Pong, Nga Hin Henry; Leung, Ting Fan

    2017-01-01

    Household animal dander has been implicated as aeroallergen in childhood atopic diseases. Many parents seek healthcare advice if household pet keeping may be detrimental in atopic eczema (AE), allergic rhinitis and asthma. We investigated if skin sensitization by cat/dog dander was associated with disease severity and quality of life in children with AE. Demographics, skin prick test (SPT) results, disease severity (Nottingham eczema severity score NESS), Children Dermatology Life Quality Index (CDLQI), blood IgE and eosinophil counts of a cohort of AE patients were reviewed. 325 AE patients followed at a pediatric dermatology clinic were evaluated. Personal history of asthma was lowest (20%) in the dog-dander-positive-group but highest (61%) in bothcat- and-dog-dander-positive group (p=0.007). Binomial logistic regression ascertained that catdander sensitization was associated with increasing age (adjusted odds ratio [aOR], 1.056; 95% Confidence Interval [CI], 1.006 to 1.109; p=0.029), dust-mite sensitization (aOR, 4.625; 95% CI, 1.444 to 14.815; p=0.010), food-allergen sensitization (aOR, 2.330; 95% CI, 1.259 to 4.310; p=0.007) and keeping-cat-ever (aOR, 7.325; 95% CI, 1.193 to 44.971; p=0.032); whereas dogdander sensitization was associated with dust-mite sensitization (aOR, 9.091; 95% CI, 1.148 to 71.980; p=0.037), food-allergen sensitization (aOR, 3.568; 95% CI, 1.341 to 9.492; p=0.011) and keeping-dog-ever (aOR, 6.809; 95% CI, 2.179 to 21.281; p=0.001). However, neither cat nor dog sensitization were associated with asthma, allergic rhinitis, parental or sibling atopic status, disease severity or quality of life. Physicians should advise parents that there is no direct correlation between AE severity, quality of life, asthma or allergic rhinitis with cutaneous sensitization to cats or dogs. Sensitized patients especially those with concomitant asthma and severe symptoms may consider non-furry alternatives if they plan to have a pet. Highly sensitized

  3. Adaptive genomic divergence under high gene flow between freshwater and brackish-water ecotypes of prickly sculpin (Cottus asper) revealed by Pool-Seq.

    Science.gov (United States)

    Dennenmoser, Stefan; Vamosi, Steven M; Nolte, Arne W; Rogers, Sean M

    2017-01-01

    Understanding the genomic basis of adaptive divergence in the presence of gene flow remains a major challenge in evolutionary biology. In prickly sculpin (Cottus asper), an abundant euryhaline fish in northwestern North America, high genetic connectivity among brackish-water (estuarine) and freshwater (tributary) habitats of coastal rivers does not preclude the build-up of neutral genetic differentiation and emergence of different life history strategies. Because these two habitats present different osmotic niches, we predicted high genetic differentiation at known teleost candidate genes underlying salinity tolerance and osmoregulation. We applied whole-genome sequencing of pooled DNA samples (Pool-Seq) to explore adaptive divergence between two estuarine and two tributary habitats. Paired-end sequence reads were mapped against genomic contigs of European Cottus, and the gene content of candidate regions was explored based on comparisons with the threespine stickleback genome. Genes showing signals of repeated differentiation among brackish-water and freshwater habitats included functions such as ion transport and structural permeability in freshwater gills, which suggests that local adaptation to different osmotic niches might contribute to genomic divergence among habitats. Overall, the presence of both repeated and unique signatures of differentiation across many loci scattered throughout the genome is consistent with polygenic adaptation from standing genetic variation and locally variable selection pressures in the early stages of life history divergence. © 2016 John Wiley & Sons Ltd.

  4. Evaluation of autologous serum skin test and skin prick test reactivity to house dust mite in patients with chronic spontaneous urticaria.

    Directory of Open Access Journals (Sweden)

    Zhiqiang Song

    Full Text Available BACKGROUND: Chronic spontaneous urticaria (CSU is a common skin disorder with etiology that is not well understood. METHODS: In this study, we evaluated the prevalence of autologous serum skin test (ASST and skin prick testing (SPT to house dust mite (HDM in 862 CSU cases in China. Clinical features, courses and treatment responses were also recorded. RESULTS: The prevalence of positive ASST was 46.3%, and patients aged 30-39 years had the highest positive rate (52.1%. Positive SPT to HDM was seen in 153 patients (17.7% with the highest positive rate (34.2% in patients aged 20 or less. Patients with positive ASST had higher urticaria activity scores (UAS (4.18±0.65 vs. 3.67±0.53 but lower positive rates of HDM (24.6% vs. 37.6%, as compared with those with negative ASST (odds ratio (OR 1.84, 95% CI 1.38-2.47. Patients could be categorized into four groups based on the results of ASST and SPT to HDM and patients with positive ASST and positive SPT to HDM had the highest disease activity scores, experienced higher frequencies of angioedema, diseases duration, and required higher dosage of loratadine every month, compared with other subgroups (P<0.0001. CONCLUSIONS: Patients with CSU showed varied responses of positive ASST and varied sensitivity to HDM, Patients with positive ASST and/or positive SPT had more disease activity compared with patients with negative ASST and/or negative SPT. Further classification can be made based on the result of SPT and ASST.

  5. Consumo e comportamento ingestivo de caprinos e ovinos alimentados com palma gigante (Opuntia ficus-indica Mill e palma orelha-de-elefante (Opuntia sp. = Intake and ingestive behavior of sheep and goats fed with cactus pear (Opuntia ficus-indica Mill and prickly pear (Opuntia sp.

    Directory of Open Access Journals (Sweden)

    Agenor Costa Ribeiro Neto

    2008-04-01

    Full Text Available O presente trabalho teve por objetivo avaliar o consumo e comportamento ingestivo de caprinos e ovinos alimentados com palma Gigante (Opuntia ficus-indica Mill e palma Orelhade-elefante (Opuntia sp.. Foram utilizados 20 animais mestiços, sendo dez caprinos e dez ovinos, alojados em galpão coletivo, contidos individualmente por meio de cordas, distribuídos em delineamento inteiramente casualizado, em um arranjo fatorial 2 x 2 (duas espécies animais e duas variedades de palma. As observações comportamentais foram realizadas em intervalos de 5 min. por um período de 24h. Os consumos de matéria seca, extrato etéreo, fibra em detergenteácido, carboidratos totais e carboidratos não-fibrosos foram menores (p 0,05 sobre o tempo gasto comalimentação e eficiência de alimentação. O tempo gasto com ruminação foi maior para a dieta com palma Gigante, consequentemente os animais da dieta com palma Orelha-de-elefante permaneceram mais tempo em ócio. Comparando as espécies caprina e ovina, verificou-se que aeficiência de ruminação, tanto de MS quanto de FDN, foi maior para a espécie ovina. Os animais gastaram mais tempo ruminando deitados do que em pé, mas não foi verificada diferença quanto ao lado escolhido para deitarem.The objective of this study was to evaluate the intake and ingestive behavior of sheep and goats fed with cactus pear (Opuntia ficus-indica Mill and prickly pear (Opuntia sp.. Twenty crossbred animals – 10 sheep and 10 goats – were used. They were lodged in a collective shed, contained individually with ropes, distributed using a completely randomized design, and treated with a 2 x 2 factorial arrangement (2 animal species and 2 varieties of cactus. Behavioralobservations were made at five-minute intervals during a 24-hour period. Dry matter, ether extract, acid detergent fiber, total carbohydrate and non-fibrous carbohydrate intake were reduced (p 0.05 on feeding time and feeding efficiency. The time spent with

  6. Popsicle-induced anaphylaxis due to carmine dye allergy.

    Science.gov (United States)

    Baldwin, J L; Chou, A H; Solomon, W R

    1997-11-01

    IgE-mediated hypersensitivity is a suggested mechanism to explain adverse reactions from carmine-containing products. To describe a patient who experienced anaphylaxis after ingestion of a popsicle colored with carmine and to provide additional evidence that the adverse reaction was IgE-mediated. The patient and her husband underwent skin prick tests to the popsicle and carmine. The patient also received skin prick tests and/or open oral challenge to each of the other components of the incriminated food. Topical application of cosmetics with and without carmine to the patient's forearm was also performed. To confirm carmine-specific IgE, a Prausnitz-Kustner (P-K) test was performed using the patient's husband as recipient. Twenty control subjects also were tested to carmine by skin prick test. The patient showed 4+ skin prick test responses to the popsicle and carmine. Skin prick tests and/or open oral challenge to each of the other components of the popsicle were negative. The patient's husband's and 20 control subjects' skin prick tests to carmine were negative as was the patient's husband's skin prick test to the popsicle. Skin prick test reactivity to the popsicle and carmine were successfully transferred to the patient's husband in P-K format. Cosmetics applied to the patient's forearm elicited no immediate response. The positive skin prick tests to the popsicle and carmine and the successful (P-K) transfer of skin prick test reactivity support a carmine-specific, IgE-mediated mechanism in explaining our patient's popsicle-induced anaphylaxis.

  7. House Dust Mite Prevalence in the House of Patients with Atopic Dermatitis in Mashhad, Iran

    Directory of Open Access Journals (Sweden)

    Toktam Ziyaei

    2017-06-01

    Full Text Available Background: Being exposed to house dust mites intensifies atopic dermatitis. This study has investigated the con­tamination rate with Dermatophagoides mites in patient's residential home with atopic dermatitis.Methods: In this cross-sectional study, 40 patients took part with atopic dermatitis (positive or negative for mites by prick Dermal Test. Samples were collected from 3 locations (living room, bedroom and bed by vacuum cleaner. Dust samples (transferred to freezer -20 ˚C were examined by direct method and flotation. The data were analyzed using statistical SPSS vr.20 software.Results: Twenty patients of positive prick test included 8 (40% male and 12 (60% female. The results of direct observation of mites: 7 cases (35% in bedding sheets, 6 cases (30% bedrooms' carpet, 3 cases (15% living room's carpet. Twenty patients of negative prick test included 8 (40% male and 12 (60% female. Only mites were found (5% in living room's carpets of negative prick test patients. Dermatophagoides pteronyssinus was more frequent than Dermatophagoides farinae. (98% vs 83%.Conclusion: Fifty-five percent of residential homes of prick test positive patients and only 5% of residential homes of prick test negative patients were positive for mite. Sunshine provided home had fewer mites than home where sunshine is not provided. Prick test positive patients used handmade carpets more than machine made ones. In posi­tive prick test patients, mites were found in bed sheet and bedroom’s carpet more than negative prick test patient's sheets and carpets.

  8. Clinical Observation on the Effect of Acupuncture at Yanglao (SI6) combined with Pricking Therapy in the Treatment of Ankle Sprains%养老穴针刺结合刺络法治疗踝关节扭伤疗效对照观察

    Institute of Scientific and Technical Information of China (English)

    杨晶; 马德元

    2016-01-01

    目的:观察养老穴针刺结合刺络法与常规针刺治疗踝关节扭伤的临床疗效差异。方法将156例踝关节扭伤患者分为综合组(80例)和针刺组(76例),综合组采用刺络配合养老穴针刺治疗;针刺组选用解溪、昆仑、申脉等穴进行常规针刺,每天1次,治疗5次。两组患者采用视觉模拟评分法( VAS)评分,观察两组临床疗效。结果两组治疗后VAS评分均明显降低( P<0.01),综合组VAS评分低于针刺组P<0.05。综合组治愈31例,显效42例,无效7例;针刺组分别为22例、40例、14例,综合组总体疗效优于针刺组。结论养老穴针刺结合刺络法治疗踝关节扭伤可明显缓解症状,疗效优于单纯针刺治疗。%Objective To observe the differences on clinical effect of acupuncture at Yanglao ( SI6 ) combined with pricking therapy in the treatment of ankle sprains .Methods 156 cases of ankle sprains patients were divided into comprehensive group ( 80 cases ) and acupuncture group ( 76 cases ) .The comprehensive group used acupuncture at Yanglao ( SI6 ) combined with pricking therapy .The acupuncture group selected Jiexi (ST41), Kunlun (BL60), Shenmai (BL62) and other points for regular acupuncture , once a day for 5 times.Using a visual analogue scale (VAS) score, the clinical effect of the two groups was observed .Results The VAS scores of the two methods significantly decreased (P<0.01).After treatment, VAS scores of the comprehensive group was lower than that of the acupuncture group P<0.05.In comprehensive group , 31 cases were cured , 42 cases were effective , and 7 cases were invalid .In the acupuncture group , 22 cases were cured , 40 cases were effective , and 14 cases were invalid .The comprehensive group was better than the acupuncture group . Conclusion The acupuncture at Yanglao ( SI6 ) combined with pricking therapy in the treatment of ankle sprains can significantly relieve symptoms, and is more

  9. 毫火针加刺络拔罐治疗急性踝关节扭伤疗效观察%Observations on the Efficacy of Filiform Needle Fire Acupuncture plus Pricking-cupping Bloodletting in Treating Acute Ankle Sprain

    Institute of Scientific and Technical Information of China (English)

    莫爱群; 李桂玲; 萧汉达

    2017-01-01

    目的 观察毫火针加刺络拔罐治疗青少年学生急性踝关节扭伤的疗效.方法 将100例急性踝关节扭伤的青少年学生患者随机分为治疗组和对照组,每组50例.治疗组进行毫火针及刺络拔罐治疗,火针及拔罐位置为踝关节压痛最明显的部位,隔天治疗1次;对照组进行常规保护、休息、冰敷、加压、抬高患肢处理.记录入选时和治疗1、3、5 d,2星期、4星期、8星期后肿胀指数评分,AOFAS评分,VAS评分变化并比较疗效.结果 治疗后两组的肿胀指数评分、AOFAS评分、VAS评分均较治疗前明显改善(P<0.05),且治疗后治疗组评分低于对照组(P<0.05).治疗组总有效率为100%(50/50),优于对照组的80%(40/50,P<0.05).结论 毫火针加刺络拔罐治疗为急性踝关节扭伤的较佳疗法,能短时间内减少患者的疼痛、肿胀,缩短治疗时间,在短时间内使其踝关节活动正常,从而减少后遗症的发生.%Objective To investigate the efficacy of filiform needle fire acupuncture plus pricking-cupping bloodletting in treating acute ankle sprain in adolescent students.Method One hundred adolescent student outpatients with acute ankle sprainwere randomly allocated to treatment and control groups, 50 cases each.The treatment group received filiform needle fire acupuncture plus pricking-cupping bloodletting at the most obviouspart of ankle tenderness once every other day. The control group received routine protection, a rest, ice compress,compressionand affected limb elevation.Swelling severitywas examined and the AOFAS Ankle Hindfoot Scalescore and the VAS score were recorded at study entry, and one, three and five days and two, four and eight weeks after treatment.Result Swelling severityand the ankle pain score improved in both groups after treatment (P<0.05) and the post-treatment score was lower in the treatment group than in the controlgroup(P<0.05). The total efficacy rate was 100% (50/50) in the treatment group

  10. Embryo sac development in some representatives of the tribe Cynodonteae (Poaceae

    Directory of Open Access Journals (Sweden)

    A. Strydom

    1994-10-01

    Full Text Available Chloris virgata Sw., Cynodon dactylon (L. Pers., Harpochloa falx (L. f. Kuntze, and Tragus berteronianus Schult. have a Polygonum type of embryo sac development. Unreduced embryo sacs were found in Eustachys paspaloides (Vahl Lanza & Mattei,  Harpochloa falx, and  Rendlia altera (Rendle Chiov. Both facultative and obligate apomixis were observed. The Hieracium type of embryo sac development was observed in the aposporic specimens.

  11. Embryo sac development in some representatives of the tribe Cynodonteae (Poaceae)

    OpenAIRE

    A. Strydom; J. J. Spies

    1994-01-01

    Chloris virgata Sw., Cynodon dactylon (L.) Pers., Harpochloa falx (L. f.) Kuntze, and Tragus berteronianus Schult. have a Polygonum type of embryo sac development. Unreduced embryo sacs were found in Eustachys paspaloides (Vahl) Lanza & Mattei,  Harpochloa falx, and  Rendlia altera (Rendle) Chiov. Both facultative and obligate apomixis were observed. The Hieracium type of embryo sac development was observed in the aposporic specimens.

  12. Evaluation of the Frequency of Food and Aeroallergens in Patients with Eczema and Urticaria in Province of Bushehr: Based on Skin Prick Test Reactivity

    Directory of Open Access Journals (Sweden)

    Shokrollah Farrokhi

    2014-10-01

    Full Text Available Background: In recent years frequency of skin allergic diseases such as atopic dermatitis (eczema and urticaria is reported to be high in the region. Identification of reactive allergens in different areas are very important in the diagnosis and treatment of these diseases. The aim of this study was to determine frequency of common allergens in patients with skin allergies in province of Bushehr with regards to eczema and urticaria. Materials and methods: In this cross-sectional study, 234 patients with urticarial and eczema were enrolled. The participants reacted to at least one allergen with SPT. Skin prick test with standard inhaled and food was performed on patients according to the herbal geography of the area. Results: Among 837 patients referred to allergy clinic, 91 patients had eczema and 143 had urticaria. The frequency of both eczema and urticaria was significantly higher in females than in males. The most common food allergens in patients with eczema were almond (56.6%, walnut (47.7% and soybean (46.1%. And in patients with urticaria were almond (58%, walnut (53.1% and tomato (48.2%. Also, aeroallergens in the subjects were house dust mite (HDM (63.7%, Russian thistle (57.7% and Alternaria alternara (51.6%. Meanwhile, the common aeroallergens in the patients were HDM (66.4%, Russian thistle (52.4% and date palm (51%. Conclusion: Our findings indicated that almond and walnut are important food allergens in participants with eczema and urticaria. Moreover, SPT reactivity was positive with aeroallergens such as HDM and weeds.

  13. Environmental Assessment: Black-Tailed Prairie Dog Management Cannon Air Force Base and Melrose Air Force Range, New Mexico

    Science.gov (United States)

    2005-12-01

    requirements are met by metabolizing grazed vegetation. Prairie dogs dig burrows to an average depth of 2-3 meters with some tunnels interconnecting with...the potential to impact non- target species such as mice, kangaroo rats, and some songbirds. Establishing control zones at CAFB and MAFR could not be...Gutierrezia sarothrae), and Russian thistle (Salsola iberica). Water requirements are met by metabolizing grazed vegetation. Prairie dogs dig burrows

  14. Vegetation Resources of Rocky Mountain Arsenal, Adams County, Colorado

    Science.gov (United States)

    1989-10-01

    SUCCULENTS Opuntia polyacantha Plains Prickly Pear Cactaceae Table 4 .(cont’d.) Scientific Name Common Name Family Name Yucca glauca Spanish Bayonet Agavaceae...Lycium halimitolium Matrim~ony Bush Solanceae Salix exigua Coyote Willow Salicaceae CACTI AND SUCCULENTS Coryphantha vivipara Ball Cactus Cactaceae ...Qpuntia compressa Prickly Pear Cactus Cactaceae Opuntia polyacantha Plains Prickly Pear Cactaceae Yucca glauca Spanish Bayonet Agavaceae cq Nl Nl Co~ V c0

  15. ON PHYTOCOENOTICAL MAPPING OF CASPIAN DESERT REGION

    Directory of Open Access Journals (Sweden)

    I. SAFRONOVA

    2004-05-01

    Full Text Available The phytoecological map (l :2.500.000 for Desert Region, including the Caspian Lowland and the Mangyshlak. has been compiled. It gives an idea of latitudinal differentiation cf vegetation. Edaphic variants and lithological composition in low mountains. The legend has been constructed according to zonal-typological principle e using an ecological-phytocoenotic classification. Heterogeneity of vegetation is reflected by means of territoria1 units (complex, series, combination and additional marks above the vegetation background. In the northern subzone vegetation is fairly monotonous and characterized by prevalence of wormwood communities (Artemisia of subgenus Seriphidium, joined in three formations: Artemisia lerchiana, A. arenaria. A. pauciflora. Small areas are occupied by shrub deserts of Calligollum aphyllum and Tamarix ramosissima. To southward of 47° N in the middle subzone on the Caspian Lowland the communities of halophyte perennial saltworts essential1y dominate, and to less extent-wormwood communities of hemipsammophytic Artemisia terrae-albae and psammophytic Artemisia arenaria and A. lerchiana. Deserts of Mangyshlak are much diverse. Dwarf semishrubs are presented by species of perennial saltworts (Anabasis salsa, Nanophyton erinaceum,Arthrophytum lehnwnianum, Salsola orientaUs and wonnwood (Artemisia terrae-albae, A. gurganica. A. santolina. To southward of 43° N in the southern subzone dwarf semishrub Salsola gemmascens and Artemisia kemrudica corrnnunities prevail.

  16. ON PHYTOCOENOTICAL MAPPING OF CASPIAN DESERT REGION

    Directory of Open Access Journals (Sweden)

    I. SAFRONOVA

    2004-01-01

    Full Text Available The phytoecological map (l :2.500.000 for Desert Region, including the Caspian Lowland and the Mangyshlak. has been compiled. It gives an idea of latitudinal differentiation cf vegetation. Edaphic variants and lithological composition in low mountains. The legend has been constructed according to zonal-typological principle e using an ecological-phytocoenotic classification. Heterogeneity of vegetation is reflected by means of territoria1 units (complex, series, combination and additional marks above the vegetation background. In the northern subzone vegetation is fairly monotonous and characterized by prevalence of wormwood communities (Artemisia of subgenus Seriphidium, joined in three formations: Artemisia lerchiana, A. arenaria. A. pauciflora. Small areas are occupied by shrub deserts of Calligollum aphyllum and Tamarix ramosissima. To southward of 47° N in the middle subzone on the Caspian Lowland the communities of halophyte perennial saltworts essential1y dominate, and to less extent-wormwood communities of hemipsammophytic Artemisia terrae-albae and psammophytic Artemisia arenaria and A. lerchiana. Deserts of Mangyshlak are much diverse. Dwarf semishrubs are presented by species of perennial saltworts (Anabasis salsa, Nanophyton erinaceum,Arthrophytum lehnwnianum, Salsola orientaUs and wonnwood (Artemisia terrae-albae, A. gurganica. A. santolina. To southward of 43° N in the southern subzone dwarf semishrub Salsola gemmascens and Artemisia kemrudica corrnnunities prevail.

  17. Taurine is absent from amino components in fruits of Opuntia ficus-indica.

    Science.gov (United States)

    Ali, Hatem Salama Mohamed; Al-Khalifa, Abdulrahman Saleh; Brückner, Hans

    2014-01-01

    Juices of edible fruits from Opuntia ficus-indica (L.) Miller, commonly named prickly pears or Indian figs, were analysed for amino acids using an automated amino acid analyser run in the high-resolution physiological mode. Emphasis was put on the detection of free taurine (Tau), but Tau could be detected neither in different cultivars of prickly pears from Italy, South Africa and the Near East nor in commercially available prickly pear juices from the market.

  18. Digestibilidad in situ de dietas con harina de nopal deshidratado conteniendo un preparado de enzimas fibrolíticas exógenas In situ digestibility in dehydrated ground prickly pear diets containing a fybrolitic enzymes product

    Directory of Open Access Journals (Sweden)

    Marco Medina Romo

    2006-07-01

    Full Text Available Se evaluó el efecto de un preparado de enzimas fibrolíticas exógenas (celulasas y xilanasas en la degradabilidad in situ de la materia seca (DisMS, fibra detergente neutro (DFDNr y fibra detergente ácido residual (DFDAr, en dietas altas o bajas en harina de nopal deshidratado. Se aplicaron concentraciones de 0, 1, 2 y 3 g de enzima por kilogramo de materia seca al inicio y 24 horas antes de la degradación in situ. Se determinó la concentración de ácidos grasos volátiles totales y de nitrógeno amoniacal a las 0, 3, 6, 9, 12 y 24 horas después de aplicarse la enzima. No se observaron efectos en DisMS, DFDNr y DFDAr; la aplicación al inicio de la degradación in situ mostró valores más altos que a 24 horas para DisMS y DFDNr, pero fue menor para DFDAr. No se observaron diferencias en las interacciones entre niveles de enzima, tipo de dieta y tiempo de pretratamiento. La aplicación de 1 y 3 g de enzima, en la dieta con bajo contenido de harina de nopal, tuvo efectos en el incremento de los ácidos grasos volátiles totales; para el nitrógeno amoniacal, los mejores resultados ocurrieron con 0 y 1 g de enzima.It was evaluated the effect of a fybrolitic enzyme product (cellulases and xylanases on in situ digestibility of dry matter (DisMS, residual neutral detergent fiber (DFDNr and acid detergent fiber (DFDAr, in dehydrated ground prickly pear diets with a low or high level. Enzyme concentrations of 0, 1, 2, and 3 g kg-1 of dry matter applied at the beginning (0 hour and 24 hours before starting in situ digestibility were used. Total volatile fatty acids and ammonia nitrogen were determined at: 0, 3, 6, 9, 12, and 24 hours after the enzyme application. There were no effects on DisMS, DFDNr, and DFDAr. Initial application of enzyme concentrations (0 hour was higher than 24 hours for DisMS and DFDNr but lower for DFDAr. No differences were observed in interactions among enzyme level, diet and application time. Application of 1 and 3 g of

  19. GA(2)LEN skin test study II: clinical relevance of inhalant allergen sensitizations in Europe

    DEFF Research Database (Denmark)

    Burbach, G J; Heinzerling, L M; Edenharter, G

    2009-01-01

    BACKGROUND: Skin prick testing is the standard for diagnosing IgE-mediated allergies. A positive skin prick reaction, however, does not always correlate with clinical symptoms. A large database from a Global Asthma and Allergy European Network (GA(2)LEN) study with data on clinical relevance was ...... the clinical relevance of positive skin prick tests and calls for further studies, which may, ultimately, help increase the positive predictive value of allergy testing.......BACKGROUND: Skin prick testing is the standard for diagnosing IgE-mediated allergies. A positive skin prick reaction, however, does not always correlate with clinical symptoms. A large database from a Global Asthma and Allergy European Network (GA(2)LEN) study with data on clinical relevance...... was used to determine the clinical relevance of sensitizations against the 18 most frequent inhalant allergens in Europe. The study population consisted of patients referred to one of the 17 allergy centres in 14 European countries (n = 3034, median age = 33 years). The aim of the study was to assess...

  20. Our experience with atopy patch tests with aeroallergens.

    Science.gov (United States)

    Celakovská, Jarmila; Ettlerová, Kvetuse; Ettler, Karel; Vanecková, Jaroslava

    2013-01-01

    Aim of our study was to evaluate the importance of atopy patch testing with aeroallergens as a diagnostic method in patients suffering from atopic dermatitis. The complet dermatological and allergological examinations were performed in 29 patients; 10 men, 19 women with the average age of 27.8 years, min. 17, max. 57 years; with the median SCORAD 24.2 points, s.d. 13.3 points. Wormwood, grass, dog dander, cat dander, dermatophagoides pharinae, dermatophagoides pteronyssinus and birch pollen were examined in diagnostic procedures. Skin prick tests, specific IgE were examined; the atopy patch tests were performed with aeroallergens for skin prick tests in concentration 1 x skin prick tests. Specific IgE and skin prick tests to one or more tested aeroallergens were positive altogether in 27 patients; atopy patch tests were positive only in one of these patients. For atopy patch testing with aeroallergens the concentration of 1 x skin prick tests is low to confirme the eczematic reaction in patients suffering from allergy to inhallant allergens.

  1. Evaluation of Banana Hypersensitivity Among a Group of Atopic Egyptian Children: Relation to Parental/Self Reports

    OpenAIRE

    El-Sayed, Zeinab A.; El-Ghoneimy, Dalia H.; El-Shennawy, Dina; Nasser, Manar W.

    2013-01-01

    Purpose To evaluate the frequency of banana sensitization and allergy among a group of atopic Egyptian children in relation to parental/self reports. Methods This is a case-control study included 2 groups of allergic children with and without history of banana allergy, each included 40 patients. They were subjected to skin prick test (SPT) using commercial banana allergen extract and prick-prick test (PPT) using raw banana, in addition to measuring the serum banana-specific IgE. Oral banana c...

  2. 2012 Anthropometric Survey of U.S. Army Pilot Personnel: Methods and Summary Statistics

    Science.gov (United States)

    2016-05-01

    center of the nipple on men Tibia - the shin bone Tragion - the juncture of the top of the cartilaginous flap of the ear with the head Tragus...of 977 men and 42 women. An augmented database of 395 women is reported for design purposes. Pilots of unmanned aircraft were not considered in this...sample here, which represents the Total Army, was composed of 977 men and 42 women, measured between November 2010 and April 2012. Results of the

  3. Consumo e comportamento ingestivo de caprinos e ovinos alimentados com palma gigante (Opuntia ficus-indica Mill e palma orelha-de-elefante (Opuntia sp. - DOI: 10.4025/actascianimsci.v30i2.4684 Intake and ingestive behavior of sheep and goats fed with cactus pear (Opuntia ficus-indica Mill and prickly pear (Opuntia sp. - DOI: 10.4025/actascianimsci.v30i2.4684

    Directory of Open Access Journals (Sweden)

    Valéria Louro Ribeiro

    2008-08-01

    Full Text Available O presente trabalho teve por objetivo avaliar o consumo e comportamento ingestivo de caprinos e ovinos alimentados com palma Gigante (Opuntia fícus-indica Mill e palma Orelha-de-elefante (Opuntia sp.. Foram utilizados 20 animais mestiços, sendo dez caprinos e dez ovinos, alojados em galpão coletivo, contidos individualmente por meio de cordas, distribuídos em delineamento inteiramente casualizado, em um arranjo fatorial 2 x 2 (duas espécies animais e duas variedades de palma. As observações comportamentais foram realizadas em intervalos de 5 min. por um período de 24h. Os consumos de matéria seca, extrato etéreo, fibra em detergente ácido, carboidratos totais e carboidratos não-fibrosos foram menores (p 0,05 sobre o tempo gasto com alimentação e eficiência de alimentação. O tempo gasto com ruminação foi maior para a dieta com palma Gigante, consequentemente os animais da dieta com palma Orelha-de-elefante permaneceram mais tempo em ócio. Comparando as espécies caprina e ovina, verificou-se que a eficiência de ruminação, tanto de MS quanto de FDN, foi maior para a espécie ovina. Os animais gastaram mais tempo ruminando deitados do que em pé, mas não foi verificada diferença quanto ao lado escolhido para deitarem.The objective of this study was to evaluate the intake and ingestive behavior of sheep and goats fed with cactus pear (Opuntia ficus-indica Mill and prickly pear (Opuntia sp.. Twenty crossbred animals – 10 sheep and 10 goats – were used. They were lodged in a collective shed, contained individually with ropes, distributed using a completely randomized design, and treated with a 2 x 2 factorial arrangement (2 animal species and 2 varieties of cactus. Behavioral observations were made at five-minute intervals during a 24-hour period. Dry matter, ether extract, acid detergent fiber, total carbohydrate and non-fibrous carbohydrate intake were reduced (p 0.05 on feeding time and feeding efficiency. The time

  4. Incidence and host determinants of work-related rhinoconjunctivitis in apprentice pastry-makers.

    Science.gov (United States)

    Gautrin, D; Ghezzo, H; Infante-Rivard, C; Malo, J-L

    2002-10-01

    The authors recently assessed the incidence and determinants of immunologic sensitization to flour in apprentice pastry-makers. The aim of this work was to determine the incidence of work-related rhinoconjunctivitis (RC) symptoms and their determinants. For this 188/230 entrants (81.7%) were evaluated before starting exposure to flour, and again 10.8 and 16.8 months after. Questionnaires and skin prick testing to common and work-related allergens were administered at each visit. Bronchial responsiveness to methacholine was assessed at baseline in all subjects and in a subgroup at follow-up. Thirty subjects (16.1%) reported new work-related RC symptoms (13.1 per 100 person-years); in three subjects (1.6%), these were accompanied by incident skin prick test reactivity to flour-derived allergens. Skin prick test reactivity to grass pollens (OR = 3.0, 95% CI, 1.3-6.7) and to pets (OR = 2.5, 95% CI, 1.1-5.9), persistent rhinitis (OR = 3.1, 95% CI, 1.1-8.4), seasonal RC (OR = 2.5, 95% CI, 1.1-5.5), RC on contact with pets (OR = 2.3, 95% CI, 1.03-5.0) and skin prick test reactivity to wheat flour (OR = 10.5, 95% CI, 2.3-46.8), assessed at baseline, were significantly associated with the incidence of work-related RC symptoms. Multivariate logistic regression analysis yielded significant OR of skin prick test reactivity to wheat flour at baseline (OR = 7.1, 95% CI, 1.7-35.1) and persistent rhinitis (OR = 3.9, 95% CI, 1.01-9.6) for the incidence of work-related RC symptoms. Increased bronchial hyperresponsiveness at follow-up was more frequent, although not significantly, in subjects positive to skin prick test to flour on entry and reporting new work-related symptoms (3/5), than in other subjects (4/17). The incidence of work-related RC symptoms among apprentice pastry-makers was high (16.1% 30/186), while a minority (3/30) also developed skin prick test reactivity to flour. Skin prick test reactivity to wheat flour and persistent allergic rhinitis on starting exposure to

  5. Currant allergy and the Rosaceae-grass pollen allergy syndrome: a case report.

    Science.gov (United States)

    Pérez-Ezquerra, Patricia Rojas; de la Gaspar, Maria Vazquez Torre; de Fernández, Manuel Barrio; Flores, Vanesa Tovar; Alvarez-Santullano, Ana Villanueva; de Ocáriz, Maria Luisa Baeza Ochoa

    2007-05-01

    Despite the increasing use of currants in culinary recipes, currant allergy has rarely been reported. To study a case of currant allergy and to explore cross-reactivity between grass pollen and Rosaceae family fruit allergens. Skin prick tests to pollen and skin prick-to-prick tests with currants and peach were performed. Specific IgE levels were determined using the CAP method. We prepared a protein extract of 0.1 mg/mL in phosphate-buffered saline using red currant in the presence of protease inhibitors. Immunoblot inhibition studies were performed to explore cross-reactivity between grass pollen and currant allergens. Skin prick test results were positive to Dactylis, arizonic, and olive pollens. Results of skin prick-to-prick tests with fresh red and black currants were negative and positive, respectively, to peach. The specific IgE level was 5.7 KU/L to red currant and 2.92 KU/L to peach (CAP). Western blot analysis with red currant extract revealed specific IgE protein bands of 37 and 26 kDa. Preincubation of sera with extracts from red currant and peach inhibited both IgE bands, and preincubation with Dactylis pollen inhibited the 37-kDa band only. We report a case of allergy to grass pollen with an oral allergy syndrome involving several fruits from 2 different families of the Rosidae subclass confirmed by in vitro tests. Inhibition studies demonstrated cross-reactivity between different fruits (currant and raspberry) from the Rosidae subclass and were incomplete with grass pollen allergens.

  6. Oral Allergy Syndrome in a Child Provoked by Royal Jelly

    Directory of Open Access Journals (Sweden)

    Fantini Paola

    2014-01-01

    Full Text Available Royal jelly has been demonstrated to have several physiological activities. However, in the literature, different reactions induced by royal jelly are reported. We describe a case of seven-year-old child that was referred to our observation for two episodes of oral allergy syndrome (OAS that appeared ten minutes after ingestion of royal jelly. Skin prick test with standard panel of inhalant and food allergens, a prick-to-prick test using the royal jelly’s extract responsible for patient’s reactions, and royal jelly patch test with extemporaneous preparation were performed. The specific IgE by ImmunoCAP System method versus Hymenoptera venom, inhalant allergens, food allergens, and lipid transfer proteins was dosed. According to the positive reactions to royal jelly both by prick-by-prick test and by a first reading patch test, royal jelly immediate hypersensitivity was diagnosed. According to the positive response for almond in both in vivo and in vitro tests we can think of the royal jelly contamination with almond pollen as possible cause of patient’s reaction. Moreover, from the results of specific IgE titers versus Compositae pollens, we have argued the possibility that this case of royal jelly allergy could be explained also by the mechanism of cross-reaction with Compositae pollens.

  7. THEORETICALLY POSSIBLE AND PRACTICALLY RELIZABLE PRODUCTIVITY OF THE LIGHT-CHESTNUT SOILS OF THE NORTHERN WEST PRECASPIAN REGION ACORDING TO THE MOISTURE AND SOLINITY (ON EXAMPLE OF KOCHUBEY BIOSPHERE STATION OF PIBR DNC RAS

    Directory of Open Access Journals (Sweden)

    G. N. Hasanov

    2014-01-01

    Full Text Available Abstract. The article presents the productivity data, the species composition and utilization rates phytocenoses FAR depending on theintegrals of dryness and moisture climate volatility, coefficient of moisture and dynamics of harmful salts in the soil according to years and seasons of the year.Methods.The studies were conducted on light-chestnut carbonate saline soil of Kochubey biosphere stations on the territory of the Terek- Kuma Lowland Precaspianregion in 2011–2013. The calculation of the use of FAR was conducted using the formula A.A. Nichiporovich to determine the theoretically possible yield of plants. Klimatogrammy during these years has been compiled by the method Walter. Stocks above and below ground plant matter into account by the method of A.A.Titlyanova.The names of species given by S.K. Cherepanov.Results. According to the results of our observations, the most important for achieving high productivity ephemeral synusia under these conditions include precipitation for April and May. Between the amount of rainfall in April and May and the productivity of abovegroundphytomass ofephemera direct correlation exists, which in 2011 had a strong, and in the next two years-the average severity. In 2012 the volatility increased, KU fell in 5 times. Such weather conditions contributed to the rise of water-soluble salts to the upper soil horizons and substantial change in species composition phytocenoses. The content Cl- in the layer 0–20 cm over the same period 2011 increased in 3.9 times, SO4--- 1.7 in times. If in 2011 the stepen soil salinity in the layer 0–35 cm characterized as weak, in 2012 the average at the same chloride- sulphate type of salinity. Obviously , an increase in the content of Cl- - ions and its relationship to the S04-- contributed to a dramatic increase in productivity and thistle herbs in 2012. Luxuriant growth of Salsola iberica Sennen et Pau particularly stood.Thus, the formation of a biomass

  8. Techniques for creating inconspicuous face-lift scars: avoiding visible incisions and loss of temporal hair.

    Science.gov (United States)

    Kridel, Russell W H; Liu, Edmund S

    2003-01-01

    Patients seeking rhytidectomy desire an improved neckline, jawline, and midface, but rarely at the price of signs that betray a face-lift, namely, visible incisions. We describe our face-lift incisional planning and the rationale behind specific surgical maneuvers for preventing unwanted sequelae. The telltale signs of poorly placed incisions include temporal hair loss, conspicuous scars, an unnatural appearance to the tragus, and a posterior hairline distorsion. Special considerations are given to the male patient and to the salvage of readily visible incision lines from previous surgery.

  9. ANALYSIS OF DWARF MISTLETOE ARCEUTHOBIUM OXYCEDRI (DC. M. BIEB. AND ITS PRINCIPAL HOST EASTERN PRICKLY JUNIPER JUNIPERUS DELTOIDES R. P. ADAMS DISTRIBUTION IN CRIMEA USING GIS TECHOLOGIES

    Directory of Open Access Journals (Sweden)

    O. V. Kukushkin

    2017-03-01

    Full Text Available The current study highlights the distribution pattern of juniper dwarf mistletoe (Arceuthobium oxycedri, a semi-parasite of the Eastern prickly juniper (Juniperus deltoides, in Crimea. A. oxycedri has considerably narrower range in Crimea as compared to its principal host and its ubiquitous distribution is rather sporadic. Nature observations characterize A. oxycedri as a thermophilic and mezo-хerophytic species confined to the low-mountain terrains with mild sub-Mediterranean climate. Significant sites of permanent infection have been discovered at the Crimean coast and in the warmest southwestern part of the Crimean Mountains to the south from the Belbek River valley. Greek juniper (J. excelsa is a codominant species growing side by side with J. deltoids in the majority of localities examined that have the high infection rate. Generally, J. excelsa is an insusceptible species in relation to the parasite; nevertheless, it is affected by A. oxycedri at several sites. Birds feeding habit to consume J. excelsa and J. deltoides fleshy berry-like cones helps to maintain the high infection rate and to disseminate mistletoe seeds at the distance of approximately 4 km. Modeling ecological niche and creating maps of potential range of the parasite and its principal host using MaxEnt 3.3.3k software have demonstrated that A. oxycedri distribution in Crimea at present may be wider than it has been currently observed. It is noteworthy that while modeling such bioclimatic indicators as the minimum winter temperatures and the elevation above sea level were irrelevant for establishing the distribution range of the parasite. Presumably the limited distribution of A. oxycedri can be attributed to the history of forming J. deltoides range in the late Pleistocene – Holocene, alongside with a low speed of the parasite dissemination from Quaternary refugia in the southernmost part of the Crimean Peninsula.

  10. The identification and syndromic management of snakebite in South ...

    African Journals Online (AJOL)

    The identification of snakebite injury is uncertain, especially in the 40% of patients who do not see the offending snake, unless there are paired fang marks or typical findings of an envenomation syndrome. The differential diagnosis would include a thorn prick, spider bite or scorpion sting. Thorn pricks are not associated with ...

  11. Evaluation of the rapid plasma reagin "teardrop" card test for screening of syphilis in field conditions.

    Science.gov (United States)

    Van Dyck, E; Van de Velden, L; Ndoye, I; Piot, P; Meheus, A

    1993-01-01

    The availability of simple diagnostic methods may contribute to more efficient control of sexually transmitted diseases (STDs) in developing countries. For the detection of syphilis, a simple rapid plasma reagin (RPR) "teardrop" assay for finger-prick blood samples was developed in 1962. The reliability of this test is compared with RPR, Treponema pallidum hemagglutination assay (TPHA), and fluorescent treponemal antibody absorption (FTA-Abs) assays performed on venous blood samples. To evaluate the potential usefulness of the finger-stick RPR teardrop assay for diagnosis of syphilis in settings with poor medical resources. Pregnant women evaluated at two health centers in Pikine, Senegal were tested for STDs. The RPR teardrop assay was performed on plasma from blood samples obtained by finger prick, and standard RPR, TPHA, and FTA-Abs procedures were performed on serum obtained by vein puncture. The sensitivity and specificity of the finger-prick RPR teardrop assay were 69.7% and 96.5%, respectively, and its reactivity was correlated with RPR serum antibody titer. The finger-prick RPR teardrop assay is not a reliable alternative to the classic serum RPR test.

  12. [Practice patterns in Mexican allergologists about skin tests with allergens during 2005-2006].

    Science.gov (United States)

    Larenas Linnemann, Désirée; Fogelbach, Guillermo Arturo Guidos; Cruz, Alfredo Arias

    2008-01-01

    Immunotherapy has been practiced since over a hundred years. The exact composition of the immunotherapy concentrate, with which the patient is treated, depends partly on the results of the skin prick tests applied to the allergic patient. As such, the effectiveness of the immunotherapy depends heavily on the quality of the skin prick test. The detailed recommendations for the realization of the skin prick tests have evolved and changed over the years, leading to multiple variations in its application in Mexico. We tried to get a picture of the daily practice patterns of the members of CMICA and CoMPedIA concerning the application of skin prick tests. Aquestionnaire was sent in various occasions to all members of the Colegio Mexicano de Inmunologia Clinica y Alergia (CMICA) and of the Colegio Mexicano de Pediatras, Especialistas en Inmunologia y Alergia (CoMPedIA). The results are presented descriptively and by calculation of the frequency/percentages of intervals of replies, in the case of numerical responses. A response rate of 61 (17%) was obtained of the College members, showing consistency in some replies but a wide variation in others, for example in the time certain medication has to be suspended before the execution of the skin prick test. Comparing the replies obtained with recent recommendations in international publications, some discrepancy can be detected. In some aspects of the survey there is coincidence of the skin test practices among the participants; however, in other items there is an important variation.

  13. Micromorphology of cactus-pear (Opuntia ficus-indica (L.) Mill) cladodes based on scanning microscopies.

    Science.gov (United States)

    Ben Salem-Fnayou, Asma; Zemni, Hassène; Nefzaoui, Ali; Ghorbel, Abdelwahed

    2014-01-01

    Cladode ultrastructural features of two prickly and two spineless Opuntia ficus-indica cultivars were examined using environmental scanning electron and atomic force microscopies. Observations focused on cladode as well as spine and glochid surface micromorphologies. Prickly cultivars were characterized by abundant cracked epicuticular wax deposits covering the cladode surface, with an amorphous structure as observed by AFM, while less abundant waxy plates were observed by ESEM on spineless cultivar cladodes. Further AFM observations allowed a rough granular and crystalloid epicuticular wax structure to be distinguished in spineless cultivars. Regarding spine micromorphology, prickly cultivars had strong persistent spines, observed by ESEM as a compact arrangement of oblong epidermal cells with a rough granular structure. However, deciduous spines in spineless cultivars had a broken transversely fissured epidermis covering a parallel arrangement of fibres. Through AFM, the deciduous spine surface presented an irregular hilly and smooth microrelief while persistent spines exhibited rough helical filamentous prints. ESEM and AFM studies of cladode surfaces from prickly and spineless cactus pear cultivars revealed valuable micro-morphological details that ought to be extended to a large number of O. ficus-indica cultivars. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Rhinoplasty and facial asymmetry: Analysis of subjective and anthropometric factors in the Caucasian nose

    Directory of Open Access Journals (Sweden)

    Carvalho, Bettina

    2012-01-01

    Full Text Available Introduction: Anthropometric proportions and symmetry are considered determinants of beauty. These parameters have significant importance in facial plastic surgery, particularly in rhinoplasty. As the central organ of the face, the nose is especially important in determining facial symmetry, both through the perception of a crooked nose and through the determination of facial growth. The evaluation of the presence of facial asymmetry has great relevance preoperatively, both for surgical planning and counseling. Aim/Objective: To evaluate and document the presence of facial asymmetry in patients during rhinoplasty planning and to correlate the anthropometric measures with the perception of facial symmetry or asymmetry, assessing whether there is a higher prevalence of facial asymmetry in these patients compared to volunteers without nasal complaints. Methods: This prospective study was performed by comparing photographs of patients with rhinoplasty planning and volunteers (controls, n = 201, and by evaluating of anthropometric measurements taken from a line passing through the center of the face, until tragus, medial canthus, corner side wing margin, and oral commissure of each side, by statistical analysis (Z test and odds ratio. Results: None of the patients or volunteers had completely symmetric values. Subjectively, 59% of patients were perceived as asymmetric, against 54% of volunteers. Objectively, more than 89% of respondents had asymmetrical measures. Patients had greater RLMTr (MidLine Tragus Ratio asymmetry than volunteers, which was statistically significant. Discussion/Conclusion: Facial asymmetries are very common in patients seeking rhinoplasty, and special attention should be paid to these aspects both for surgical planning and for counseling of patients.

  15. Rhinoplasty and facial asymmetry: Analysis of subjective and anthropometric factors in the Caucasian nose.

    Science.gov (United States)

    Carvalho, Bettina; Ballin, Annelyse Christine; Becker, Renata Vecentin; Berger, Cezar Augusto Sarraff; Hurtado, Johann G G Melcherts; Mocellin, Marcos

    2012-10-01

     Anthropometric proportions and symmetry are considered determinants of beauty. These parameters have significant importance in facial plastic surgery, particularly in rhinoplasty. As the central organ of the face, the nose is especially important in determining facial symmetry, both through the perception of a crooked nose and through the determination of facial growth. The evaluation of the presence of facial asymmetry has great relevance preoperatively, both for surgical planning and counseling.  To evaluate and document the presence of facial asymmetry in patients during rhinoplasty planning and to correlate the anthropometric measures with the perception of facial symmetry or asymmetry, assessing whether there is a higher prevalence of facial asymmetry in these patients compared to volunteers without nasal complaints.  This prospective study was performed by comparing photographs of patients with rhinoplasty planning and volunteers (controls), n = 201, and by evaluating of anthropometric measurements taken from a line passing through the center of the face, until tragus, medial canthus, corner side wing margin, and oral commissure of each side, by statistical analysis (Z test and odds ratio).  None of the patients or volunteers had completely symmetric values. Subjectively, 59% of patients were perceived as asymmetric, against 54% of volunteers. Objectively, more than 89% of respondents had asymmetrical measures. Patients had greater RLMTr (MidLine Tragus Ratio) asymmetry than volunteers, which was statistically significant.  Facial asymmetries are very common in patients seeking rhinoplasty, and special attention should be paid to these aspects both for surgical planning and for counseling of patients.

  16. Relationship between head posture and lumbar curve in a sitting position: a biomechanical study

    Directory of Open Access Journals (Sweden)

    Rozilene Maria Cota Aroeira

    Full Text Available Abstract Introduction: The sitting position routinely used for a wide variety of tasks increases the potential of developing forward head posture, which can seriously compromise the health of different systems in the human body. Objective: A static equilibrium analysis was conducted, comparing the position of the head with the lumbar curve in three different sitting positions. Methods: The approximate force and flexion moment of the head extensor muscles in static equilibrium was calculated in each of the following positions: (A without a backrest; (B using a backrest with a 100° tilt angle; (C using a 100° tilted backrest associated with a cylindrical lumbar support cushion at the level of the L3 vertebra. Results: The C7-tragus angles were 43°, 50° and 52°; Frankfort horizontal plane (FH angles were 5°, 9° and 9°; force of the head extensor muscles was 53.0N, 59.7N and 43.5N and flexion moments were 2.60Nm, 2.05Nm and 1.78Nm, in positions A, B and C, respectively. Conclusion: The results revealed that the sitting position using a 100° tilted backrest and lumbar support with the smallest L3-tragus horizontal distance required less effort by the head and neck extensor muscles to retain the head in equilibrium. This study demonstrated the need to preserve the physiology of the lumbar spine, characterized by the position of the L3 vertebra, in order to ensure good head position.

  17. Micromorfologia Foliar de Espécies de Sida spp. (guanxumas Leaf Micromorphology of Sida spp. Species (prickly sida

    Directory of Open Access Journals (Sweden)

    L.H.B. Albert

    2002-12-01

    influenced by the morphological and physical-chemical characteristics of the leaf surface on which the herbicide solution is sprayed. The topography of the leaf surface, thickness of the cuticle, and the degree and type of epicuticular wax and the presence and type of trichomes, are characteristics that influence the distribution of the herbicide spray solution on the leaf surface and thus the efficacy of weed control. Therefore, the objective of this experiment was to obtain information on the morphological characteristics of three foliar surfaces of prickly sida (Sida rhombifolia, Sida glaziovii and Sida cordifolia. The experiment was conducted at the Electronic Microscopic Center Applied to Agriculture (NAP/MEPA, of Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba-SP, Brazil. The experiment using electronic microscopy technique showed that the species S. rhombifolia presented a higher amount of epicuticle-striped wax while the species S. cordifolia presented a flat and smooth cuticle. The species S. glaziovii showed the highest amount of trichomes on the adaxial foliar surface. All the species analyzed had stomata in both surfaces of the leaves, with a predominance of the anomocytic type.

  18. Asymptomatic skin sensitization to birch predicts later development of birch pollen allergy in adults

    DEFF Research Database (Denmark)

    Bodtger, Uffe; Poulsen, Lars K.; Malling, Hans-Jørgen

    2003-01-01

    The skin prick test is the allergologic test of choice, but asymptomatic skin sensitization to aeroallergens is common. However, no data in the literature describe the clinical phenotype of asymptomatic sensitized adults.......The skin prick test is the allergologic test of choice, but asymptomatic skin sensitization to aeroallergens is common. However, no data in the literature describe the clinical phenotype of asymptomatic sensitized adults....

  19. A Bayesian analysis of gene flow from crops to their wild relatives: cultivated (Lactuca sativa L.) and prickly lettuce (L. serriola L.) and the recent expansion of L. serriola in Europe.

    Science.gov (United States)

    Uwimana, Brigitte; D'Andrea, Luigi; Felber, François; Hooftman, Danny A P; Den Nijs, Hans C M; Smulders, Marinus J M; Visser, Richard G F; Van De Wiel, Clemens C M

    2012-06-01

    Interspecific gene flow can lead to the formation of hybrid populations that have a competitive advantage over the parental populations, even for hybrids from a cross between crops and wild relatives. Wild prickly lettuce (Lactuca serriola) has recently expanded in Europe and hybridization with the related crop species (cultivated lettuce, L. sativa) has been hypothesized as one of the mechanisms behind this expansion. In a basically selfing species, such as lettuce, assessing hybridization in natural populations may not be straightforward. Therefore, we analysed a uniquely large data set of plants genotyped with SSR (simple sequence repeat) markers with two programs for Bayesian population genetic analysis, STRUCTURE and NewHybrids. The data set comprised 7738 plants, including a complete genebank collection, which provided a wide coverage of cultivated germplasm and a fair coverage of wild accessions, and a set of wild populations recently sampled across Europe. STRUCTURE analysis inferred the occurrence of hybrids at a level of 7% across Europe. NewHybrids indicated these hybrids to be advanced selfed generations of a hybridization event or of one backcross after such an event, which is according to expectations for a basically selfing species. These advanced selfed generations could not be detected effectively with crop-specific alleles. In the northern part of Europe, where the expansion of L. serriola took place, the fewest putative hybrids were found. Therefore, we conclude that other mechanisms than crop/wild gene flow, such as an increase in disturbed habitats and/or climate warming, are more likely explanations for this expansion. © 2012 Blackwell Publishing Ltd.

  20. Anaphylaxis after eating Italian pizza containing buckwheat as the hidden food allergen.

    Science.gov (United States)

    Heffler, E; Guida, G; Badiu, I; Nebiolo, F; Rolla, G

    2007-01-01

    A 20-year-old woman developed anaphylaxis after eating pizza on 4 different occasions in 2 restaurants. Both restaurants made their pizza dough with a mixture of wheat and buckwheat flours. A prick-to-prick test with buckwheat flour was positive. Skin prick tests and specific immunoglobulin E responses to soybean and peanut were weakly positive while the response to buckwheat was negative. We ruled out a pathogenic role for peanut and soybean because the patient usually eats both with no signs of allergic reaction. Double-blind, placebo-controlled food challenges with buckwheat flour were positive after the administration of a cumulative dose of 2.3 g of the culprit flour. To our knowledge, our report describes the first case of anaphylaxis after intake of buckwheat flour as the hidden allergen in pizza dough.

  1. Latex Allergy In Health Care Workers

    Directory of Open Access Journals (Sweden)

    Hayriye Sarıcaoğlu

    2013-05-01

    Full Text Available Background and Design: We aimed to determine the frequency of latex allergy in our hospital and to to evaluate the clinical and demographical features of the cases.Materials and Methods: A detailed questionnaire was administered to healthcare workers by a physician. Skin prick test with latex and patch test with rubber chemicals and a piece of latex glove were performed for all healthcare workers. Latex-specific IgE was measured in serum.Results: The study sample consisted of 36 nurses, 14 doctors, and 50 healthcare workers. While 46 subjects had symptoms, 54 subjects had no symptoms. The relationship of clinical disease with working duration, exposure duration (hour/day, history of atopy, and drug/food allergies was statistically significant. Five nurses and 1 healthcare worker had positive skin prick test. Two of them had positive latex-specific IgE. Positive skin prick test statistically significantly correlated with occupation, working duration, exposure duration (hour/day and positive latex-specific IgE. Two nurses and 2 healthcare workers had positive latex-specific IgE. Two of them had positive skin prick test. Positive latexspecific IgE statistically significantly correlated with working duration, exposure duration, and positive skin prick test. Patch test with a piece of latex glove was negative in all subjects. Three healthcare workers had positive patch test with thiuram-mix, one of them had also positive patch test with mercaptobenzothiazole.Discussion: One of the risk factors for latex allergy is occupations involving frequent exposure to latex products. Latex allergy should be taken into consideration if type I hypersensitivity reactions occur in occupational groups at risk for anaphylactic reaction.

  2. A Case of Immediate Hypersensitivity Reaction to Maltitol

    Directory of Open Access Journals (Sweden)

    Ana Rodríguez Trabado

    2017-01-01

    Full Text Available Background. Maltitol is a sugar alcohol that is frequently used as a noncaloric sweetener, although it is also used as an excipient, a plasticizer in gelatin capsules, and an emollient. It has not been previously described as an agent involved in immediate hypersensitivity reactions. Methods. We report on an anaphylactoid reaction with pharyngeal occlusion suffered by a 60-year-old man after ingestion of a candy containing maltitol syrup. A prick-to-prick test was performed with the candy and maltitol powder. Other allergens were excluded as causative agents of the adverse reaction, although the patient refused to undergo an oral challenge test with the candy. A basophil activation test (BAT was performed with maltitol powder, and a dose-response curve was generated. The test was also performed in 3 healthy controls. Results. Both prick-to-prick tests were negative. The result of the BAT was positive at all the concentrations tested in the patient’s blood and negative in all the controls. Conclusions. The BAT can help to clarify the agents implicated in an adverse reaction and can reduce the risk involved in diagnosis. The BAT can also prove useful in the study of reactions caused by low-molecular-weight antigens, for which routine diagnostic tests are not feasible.

  3. Permanent make-up colorants may cause severe skin reactions.

    Science.gov (United States)

    Wenzel, Sabrina M; Welzel, Julia; Hafner, Christian; Landthaler, Michael; Bäumler, Wolfgang

    2010-10-01

    In recent years, cosmetic tattoos [permanent make-up (PMU)] on eyelids, eyebrows and lips have become increasingly popular. However, most colorants are manufactured for non-medical purposes, without any established history of safe use in humans. To investigate severe adverse reactions, such as swelling, burning, and the development of papules, of the lips and the surrounding area in 4 patients who had had at least two PMU procedures on their lips. Adverse skin reactions were examined with patch and prick testing of the colorants. In addition, skin biopsies were taken in the centre of the prick test for histology. One patient declined prick testing. Beauticians tended to use various PMU products, but all contained Pigment Red 181 (CI 73360). All patients tested showed a clear delayed reaction to Pigment Red 181 or the tattoo ink, or both, after prick testing. Histology indicated an allergic reaction. Each lip lesion slowly abated after several months of topical or systemic therapy with steroids in combination with tacrolimus, but none has yet completely resolved. In light of the severe and often therapy-resistant skin reactions, we strongly recommend the regulation and control of the substances used in PMU colorants. © 2010 John Wiley & Sons A/S.

  4. Etude physico-chimique, biochimique et stabilité d’un nouveau produit : jus de cladode du figuier de Barbarie marocain (Opuntia ficus-indica et Opuntia megacantha).

    OpenAIRE

    Boutakiout , Amale

    2015-01-01

    The orientations of the Moroccan agricultural policy concerning the Green Morocco Plan, promote the development of the arid zones and the development of crops that adapt to global warming. The prickly pear cactus is a plant which is adapted to the arid climate and still untapped in Morocco. Prickly pear cladodes contain bioactive components that have beneficial health effects. The juice extracted from cladodes of both species Opuntia ficus-indica(spineless) and Opuntia megacantha(spiny) was t...

  5. Occupational food-related hand dermatoses seen over a 10-year period

    DEFF Research Database (Denmark)

    Vester, Lotte; Thyssen, Jacob P; Menné, Torkil

    2012-01-01

    . A suggestion for diagnostic criteria is presented. Frequent risk occupations were cooking in restaurants, baking, and kitchen work. Substantially more patients reacted in skin prick testing with fresh foods than with food extracts. Conclusion. Protein contact dermatitis is a frequent disorder among patients...... who professionally handle foods, and should be considered to be a distinct clinical entity. When diagnosing protein contact dermatitis and in other food-related skin prick testing procedures, it is important to include fresh foods....

  6. Decrease in the rate of sensitization and clinical allergy to natural rubber latex

    DEFF Research Database (Denmark)

    Blaabjerg, Michelle S B; Andersen, Klaus E; Bindslev-Jensen, Carsten

    2015-01-01

    patients prick tested for NRL at the Department of Dermatology and Allergy Centre, Odense University Hospital were included in this study (n = 8580). In NRL-sensitized patients, the clinical relevance was evaluated for NRL. Furthermore, concomitant positive prick test results for birch pollen were recorded.......5-0.6% in 2006-2013 (p history of reaction to oral intake of related fruits or vegetables. CONCLUSION: Our study showed a statistically significant decline in the number of patients...

  7. Opposite Effect of Opuntia ficus-indica L. Juice Depending on Fruit Maturity Stage on Gastrointestinal Physiological Parameters in Rat.

    Science.gov (United States)

    Rtibi, Kais; Selmi, Slimen; Grami, Dhekra; Amri, Mohamed; Sebai, Hichem; Marzouki, Lamjed

    2018-06-01

    The phytochemical composition and the effect of the green and ripe Opuntia ficus-indica juice on some gastrointestinal (GI) physiological parameters such as stomach emptying and small-intestinal motility and permeability were determined in rats administered multiple concentrations of the prickly pear juice (5, 10, and 20 mL kg -1 , b.w., p.o.). Other separate groups of rats were received, respectively; sodium chloride (0.9%, b.w., p.o.), clonidine (α- 2 -adrenergic agonist, 1 mg kg -1 , b.w., i.p.), yohimbine (α- 2 -adrenergic antagonist, 2 mg kg -1 , b.w., i.p.), and loperamide (5 mg kg -1 , b.w., p.o.). In vivo reverse effect of juice on GI physiological parameters was investigated using a charcoal meal test, phenol-red colorimetric method, loperamide-induced acute constipation, and castor oil-caused small-bowel hypersecretion. However, the opposite in vitro influence of juice on intestinal permeability homeostasis was assessed by the Ussing chamber system. Mature prickly pear juice administration stimulated significantly and dose dependently the GI transit (GIT; 8-26%) and gastric emptying (0.9-11%) in a rat model. Conversely, the immature prickly pear juice reduced gastric emptying (7-23%), GIT (10-28%), and diarrhea (59-88%). Moreover, the standard drugs have produced their antagonistic effects on GI physiological functions. The permeability of the isolated perfused rat small-intestine has a paradoxical response flowing prickly pear juices administration at diverse doses and maturity grade. Most importantly, the quantitative phytochemical analyses of both juices showed a different composition depending on the degree of maturity. In conclusion, the prickly pear juice at two distinct phases of maturity has different phytochemical characteristics and opposite effects on GI physiological actions in rat.

  8. Postorgasmic Illness Syndrome (POIS) in 45 Dutch caucasian males: clinical characteristics and evidence for an immunogenic pathogenesis (Part 1).

    Science.gov (United States)

    Waldinger, Marcel D; Meinardi, Marcus M H M; Zwinderman, Aeilko H; Schweitzer, Dave H

    2011-04-01

    Postorgasmic illness syndrome (POIS) is a combination of local allergic symptoms and transient flu-like illness. In this study, the investigators propose five preliminary criteria to establish the diagnosis. To describe the clinical details in 45 males being suspected of having POIS and to test an immunogenic hypothesis as the underlying mechanism of their presentations. Forty-five males were studied according to standardized protocol, including neuropsychiatric and medical sexological evaluations; their complaints were categorized using their own words, and their self-perceived intravaginal ejaculation latency time (IELT). Skin-prick testing with autologous diluted semen in 33 men were also performed. Clinical features of POIS including self-perceived IELTs and the results of skin-prick testing with autologous diluted seminal fluid. Of the 45 included men, 33 subjects consented with skin-prick testing. Of them, 29 (88%) men had a positive skin-prick test with their own (autologous) semen, and four had a negative test. In 87% of men, POIS symptoms started within 30 minutes after ejaculation. Complaints of POIS were categorized in seven clusters of symptoms, e.g., general, flu-like, head, eyes, nose, throat, and muscles. Local allergic reactions of eyes and nose were reported in 44% and 33% of subjects, a flu-like syndrome in 78% of subjects, exhaustion and concentration difficulties in 80% and 87% of subjects. Of all subjects, 58% had an atopic constitution. Lifelong premature ejaculation, defined as self-perceived IELT < 1 minute, was reported in 25 (56%) of subjects. The combination of allergic and systemic flu-like reactions post-ejaculation together with a positive skin-prick test in the majority of males underscores the hypothesis of an "immunogenic" etiology of POIS, e.g., that POIS is caused by Type-1 and Type-IV allergy to the males' own semen, as soon it is triggered by ejaculation. © 2011 International Society for Sexual Medicine.

  9. Vicarious pain experiences while observing another in pain: an experimental approach

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    Sophie eVandenbroucke

    2013-06-01

    Full Text Available Objective: This study aimed at developing an experimental paradigm to assess vicarious pain experiences. We further explored the putative moderating role of observer’s characteristics such as hypervigilance for pain and dispositional empathy. Methods: Two experiments are reported using a similar procedure. Undergraduate students were selected based upon whether they reported vicarious pain in daily life, and categorized into a pain responder group or a comparison group. Participants were presented a series of videos showing hands being pricked whilst receiving occasionally pricking (electrocutaneous stimuli themselves. In congruent trials, pricking and visual stimuli were applied to the same spatial location. In incongruent trials, pricking and visual stimuli were in the opposite spatial location. Participants were required to report on which location they felt a pricking sensation. Of primary interest was the effect of viewing another in pain upon vicarious pain errors, i.e., the number of trials in which an illusionary sensation was reported. Furthermore, we explored the effect of individual differences in hypervigilance to pain, dispositional empathy and the rubber hand illusion (RHI upon vicarious pain errors. Results: Results of both experiments indicated that the number of vicarious pain errors was overall low. In line with expectations, the number of vicarious pain errors was higher in the pain responder group than in the comparison group. Self-reported hypervigilance for pain lowered the probability of reporting vicarious pain errors in the pain responder group, but dispositional empathy and the RHI did not. Conclusion: Our paradigm allows measuring vicarious pain experiences in students. However, the prevalence of vicarious experiences of pain is low, and only a small percentage of participants display the phenomenon. It remains however unknown which variables affect its occurrence.

  10. Low Hemoglobin among Pregnant Women in Midwives Practice of Primary Health Care, Jatinangor, Indonesia: Iron Deficiency Anemia or β-Thalassemia Trait?

    Science.gov (United States)

    Susanti, Ari Indra; Winarno, Gatot; Sugianli, Adhi Kristianto; Susanto, Herman; Panigoro, Ramdan

    2017-01-01

    Low hemoglobin (Hb) or anemia is common among pregnant women in developing countries which may cause adverse pregnancy outcomes and maternal deaths. Our study aimed to assess Hb level measured by midwives in primary health care facility at rural area of Jatinangor, Indonesia, and to explore whether the anemia was due to iron deficiency (IDA) or β-thalassemia trait (β-TT). Pregnant women (n = 105) had finger prick test for Hb level during a regular antenatal care examination from October to November 2016. Hb level by finger prick test was compared with venous blood, measured by complete blood count (CBC). Indices including MCV and MCH and indices of Shine & Lal, Mentzer, Srivastava, Engels & Frase, Ehsani, and Sirdah were analyzed to differentiate anemia due to IDA and anemia due to suspect β-TT. HbA2 was measured to confirm β-TT. Anemic pregnant women were found in 86.7% by finger prick test compared to 21.9% (n = 23) by CBC. The prevalence of β-TT in our study was 5.7%. Hb measurement among pregnant women in low resource area is highly important; however, finger prick test in this study showed a high frequency of anemia which may lead to iron oversupplementation. A standard CBC is encouraged; MCV and MCH would help midwives to identify β-TT. PMID:28634546

  11. Sensitization to Casuarina equisetifolia and Pinus spp Pollen in Patients with Allergic Rhinitis and Asthma in Mexico City

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    Andrea Aída Velasco-Medina

    2014-01-01

    Full Text Available Background: Pollinosis studies at Mexico City have found a considerable amount of Casuarina equisetifolia and Pinus spp pollen, its sensitization frequency is unknown. In Mexico, some allergens are not considered related to asthma or allergic rhinitis, even though reports in other coun- tries have been demonstrated their relevance as aeroallergens. Objective: To estimate the frequency of sensitization to Casuarina eq- uisetifolia and Pinus spp pollen. Patients and method: A transversal, descriptive trial was done at Hos- pital General de Mexico. Previous informed consent 142 patients with allergic rhinitis and asthma, 3 to 55 years old, were included to the study. A complete clinical evaluation, laboratory tests and skin prick tests were performed. Results: We included 142 patients, 44 children (64% males and 98 adults (73% females. We found that 8 (18.18% children and 35 (35.7% adults had a positive skin prick test to Casuarina equisetifolia. None of the patients included in the study had a positive skin prick test to Pinus spp. Conclusions: Sensitization to Casuarina equisetifolia is as important as other pollens found in Mexico City. These results suggest that it should be included when skin prick tests are performed. Pinus spp pollen is considered an aeroallergen in European countries but we did not cor- roborate sensitization in our population.

  12. Physicochemical properties and storage stability of margarine containing Opuntia ficus-indica peel extract as antioxidant.

    Science.gov (United States)

    Chougui, Nadia; Djerroud, Naima; Naraoui, Fatima; Hadjal, Samir; Aliane, Khellaf; Zeroual, Brahim; Larbat, Romain

    2015-04-15

    This study falls within the framework of the industrial exploitation of by-products of the prickly pear (Opuntia ficus-indica). The study aims to evaluate the use of hydro-ethanolic extract of prickly pear peels as a substitute of vitamin E used as antioxidant in margarine preservation. The extract was rich in total phenolics (1512.58 mg GAE/100 g DM). HPLC-DAD-ESI-MS(n) analyses allowed the identification of sixteen compounds belonging to hydroxybenzoic acids, hydroxycinnamic acids and flavonoids. The extract displayed a reducing power and an antiradical activity that were respectively similar to and lower than the two antioxidant standards quercetin and butylated hydroxyanisole. Tests conducted at laboratory and pilot scales showed that the margarines elaborated with peel extract were more resistant to oxidation than the margarine reference with vitamin E. In addition, neither the physicochemical nor the microbiological properties were modified. Prickly pear peels contain bioactive substances that could be used in different food sectors. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Anchored but not internalized: shape dependent endocytosis of nanodiamond

    Science.gov (United States)

    Zhang, Bokai; Feng, Xi; Yin, Hang; Ge, Zhenpeng; Wang, Yanhuan; Chu, Zhiqin; Raabova, Helena; Vavra, Jan; Cigler, Petr; Liu, Renbao; Wang, Yi; Li, Quan

    2017-04-01

    Nanoparticle-cell interactions begin with the cellular uptake of the nanoparticles, a process that eventually determines their cellular fate. In the present work, we show that the morphological features of nanodiamonds (NDs) affect both the anchoring and internalization stages of their endocytosis. While a prickly ND (with sharp edges/corners) has no trouble of anchoring onto the plasma membrane, it suffers from difficult internalization afterwards. In comparison, the internalization of a round ND (obtained by selective etching of the prickly ND) is not limited by its lower anchoring amount and presents a much higher endocytosis amount. Molecular dynamics simulation and continuum modelling results suggest that the observed difference in the anchoring of round and prickly NDs likely results from the reduced contact surface area with the cell membrane of the former, while the energy penalty associated with membrane curvature generation, which is lower for a round ND, may explain its higher probability of the subsequent internalization.

  14. Potential for Conservation Agriculture in the Dry Marginal Zone of Central Syria: A Preliminary Assessment

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    Baqir Lalani

    2018-02-01

    Full Text Available This paper reports on early soil related outcomes from conservation agriculture (CA benchmark sites located within the marginal rainfed environment of agro-ecological zone 4 (annual rainfall: 200–250 mm in pre-conflict central Syria. The outcomes reported are specifically those that relate to beneficial soil quality and water retention attributes relative to conventional tillage-based soil management practices applied to the fodder barley–livestock system, the dominant system in the zone. On-farm operational research was established to examine the impact of a barley (Hordeum vulgare and vetch (Vicia sativa rotation intercropped with atriplex (Atriplex halimus and salsola (Salsola collina, under CA and conventional tillage agriculture, on the soil quality parameters and crop productivity. Preliminary results showed that CA had a positive effect on the soil quality parameters and crop performance. The soil moisture and hydraulic conductivity were higher under CA (p < 0.05, combined with improved productivity (grain and above-ground biomass under specific crop mixes. The results suggest that despite the marginal nature of the zone, the use of CA is a viable option for the future of farmers’ livelihoods within similar localities and agro-climates, given the benefits for soil moisture and grain and straw productivity. In addition, it is likely to positively impact those in marginal environments where both pastoralism and agro-pastoralism production systems co-exist and compete for crop biomass as a main source of livestock feed. The increase in grain and straw yields vis-à-vis improvements in biophysical parameters in the CA system relative to tillage agriculture does suggest, however, that the competition with livestock for biomass is likely to reduce over time, and farmers would be able to return increased levels of straw (as stubble and residue as mulch, given improved biomass yields.

  15. Food allergy diagnosis in patients with elimination diet history. Preliminary report

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    Elsy Maureen Navarrete-Rodríguez

    2014-09-01

    Full Text Available Background: Food allergy diagnosis is performed by a double blind placebo controlled challenge; however, in a lot of patients, it is only based on clinical history, skin prick tests, or parents’ perception. There is a high frequency of elimination diets without an adequate approach. Objective: To analyze the results of diagnostic tests in a group of children with elimination diet-based on suspected food allergy and verify such studies with double blind placebo-controlled test challenge. Material and method: An observational, analytical and prospective study was done in a group of patients with elimination diet for suspected food allergy. We performed prick test, Prick-to-Prick test and patch test and the positive ones were verified by double-blind placebo-controlled challenge. Results: Fourty-three patients were included within a total of 1,935 tests. Both approach for immediate and late sensitivity had not statistically significant relationship between a positive test and the elimination of food. Until now, we had 4 (8% positive challenges out of 50. Conclusion: The frequency of allergy proved by double-blind placebo-controlled test in 50 challenges was of 8% (4/50, thus, in the preliminary report we found a high frequency of elimination diets without adequate support. It is very important that food allergy diagnosis is accurate and based on an appropriate approach; since the implementation of an elimination diet in pediatric population can have a negative influence on their growth and development.

  16. Expression Stabilities of Ten Candidate Reference Genes for RT-qPCR in Zanthoxylum bungeanum Maxim.

    Science.gov (United States)

    Fei, Xitong; Shi, Qianqian; Yang, Tuxi; Fei, Zhaoxue; Wei, Anzhi

    2018-03-30

    Real-time reverse transcription quantitative PCR has become a common method for studying gene expression, however, the optimal selection of stable reference genes is a prerequisite for obtaining accurate quantification of transcript abundance. Suitable reference genes for RT-qPCR have not yet been identified for Chinese prickly ash ( Zanthoxylum bungeanum Maxim.). Chinese prickly ash is the source of an important food seasoning in China. In recent years, Chinese prickly ash has also been developed as a medicinal plant. The expression stabilities of ten genes ( 18S , 28S , EF , UBA , UBQ , TIF , NTB , TUA , RPS , and TIF5A ) were evaluated in roots, stems, leaves, flowers and fruits at five developmental stages and also under stress from cold, drought, and salt. To do this we used three different statistical algorithms: geNorm, NormFinder and BestKeeper. Among the genes investigated, UBA and UBQ were found to be most stable for the different cultivars and different tissues examined, UBQ and TIF for fruit developmental stage. Meanwhile, EF and TUA were most stable under cold treatment, EF and UBQ under drought treatment and NTB and RPS under salt treatment. UBA and UBQ for all samples evaluated were most stably expressed, but 18S , TUA and RPS were found to be generally unreliable as reference genes. Our results provide a basis for the future selection of reference genes for biological research with Chinese prickly ash, under a variety of conditions.

  17. Modulation of type I immediate and type IV delayed immunoreactivity using direct suggestion and guided imagery during hypnosis.

    Science.gov (United States)

    Zachariae, R; Bjerring, P; Arendt-Nielsen, L

    1989-11-01

    Cutaneous reactivity against histamine skin prick test (Type I) and purified tuberculin protein derivative (Mantoux reaction, Type IV) was studied in eight volunteers under hypnosis. Types I and IV immunoreactivity were modulated by direct suggestion (Type I) and guided imagery (Type IV). The volunteers were highly susceptible subjects, selected by means of the Harvard Group Scale of Hypnotic Susceptibility, Form A. When the volunteers underwent hypnotic suggestion to decrease the cutaneous reaction to histamine prick test, a significant (P less than 0.02) reduction of the flare reaction (area of erythema) was observed compared with control histamine skin prick tests. The wheal reaction did not respond to hypnotic suggestion. Neither wheal nor flare reaction could be increased in size by hypnotic suggestion compared with control histamine skin prick tests. A hypnotic suggestion of increasing the Type IV reaction on one arm and decreasing the reaction on the other revealed a significant difference in both erythema size (P less than 0.02) and palpable induration (P less than 0.01). In two cases the reactions were monitored by laser doppler blood flowmetry and skin thickness measurement by ultrasound. The difference between the suggested increased and decreased reaction was 19% for the laser doppler bloodflow (in favor of the augmented side), and 44% for the dermal infiltrate thickness. This study objectively supports the numerous uncontrolled case reports of modulation of immunoreactivity in allergic diseases involving both Type I and Type IV skin reactions following hypnotic suggestions.

  18. Cocaine Allergy in Drug-Dependent Patients and Allergic People.

    Science.gov (United States)

    Armentia, Alicia; Martín-Armentia, Blanca; Martín-Armentia, Sara; Ruiz-Muñoz, Pedro; Quesada, Jorge Martínez; Postigo, Idoia; Conde, Rosa; González-Sagrado, Manuel; Pineda, Fernando; Castillo, Miriam; Palacios, Ricardo; Tejedor, Jesús

    Adverse reactions to local anesthetics (LAs), especially esters, are not uncommon, but true allergy is rarely diagnosed. To our knowledge, currently there is no reliable method of determining IgE-mediated hypersensitivity to LAs and cocaine. To assess the clinical value of allergy tests (prick, IgE, challenges, and arrays) in people suffering hypersensitivity reactions (asthma and anaphylaxis) during local anesthesia with cocaine derivatives and drug abusers with allergic symptoms after cocaine inhalation. We selected cocaine-dependent patients and allergic patients who suffered severe reactions during local anesthesia from a database of 23,873 patients. The diagnostic yield (sensitivity, specificity, and predictive value) of allergy tests using cocaine and coca leaf extracts in determining cocaine allergy was assessed, taking a positive challenge as the criterion standard. After prick tests, specific IgE, and challenge with cocaine extract, 41 of 211 patients (19.4%) were diagnosed as sensitized to cocaine. Prick tests and IgE to coca leaves (coca tea) had a good sensitivity (95.1% and 92.7%, respectively) and specificity (92.3 and 98.8%, respectively) for the diagnosis of cocaine allergy and LA-derived allergy. Cocaine may be an important allergen. Drug abusers and patients sensitized to local anesthesia and tobacco are at risk. Both prick tests and specific IgE against coca leaf extract detected sensitization to cocaine. The highest levels were related to severe clinical profiles. Copyright © 2017 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  19. [A comparative study on seed germination of 15 grass species in Keeqin Sandyland].

    Science.gov (United States)

    Liu, Zhimin; Li, Xuehua; Li, Rongping; Jiang, Deming; Cao, Chengyou

    2003-09-01

    A laboratory study was made on the germination characteristics of freshly-collected seeds of grass species at the Wulanaodu area of Keeqin Sandyland in Eastern Inner-Mongolia. Of the 15 species examined, 8 species including Clinelymus dahuricus, Cleistogenes squarrosa, Pappophorum boreale, Spodiopogon sibiricus, Phragmites communis, Chloris virgata, Arundinella hirta, Pennisetum alopecuroides had a germination rate of over 80%, but 4 species including Echinochloa hispidula, Hemarthria compressa, Tragus berteronianus and Setaria viridis had a value of less than 10%. Spodiopogon sibiricus, Eragrostis pilosa, Phragmites communis, Chloris virgata, Clinelymus dahuricus, Pappophorum boreale, Digitaria cilliaris and Cleistogenes squrrosa began to germinate within 1-3 days after the test began, while Setaria viridis, Tragus berteronianus and Hemarthria compressa failed to germinate in a period of more than 10 days. For the species such as Digitaria cilliaris, Echinochloa hispidula, Phragmites communis, Eragrostis pilosa and Spodiopogon sibiricus, their germination period was less than 10 days, while Clinelymus dahuricus and Pappophorum boreale had a germination period of more than 20 days. The days required for half the final germination rate to be reached were: 2 days for Chloris virgata, 3 days for Phragmites communis, 4 days for Spodiopogon sibiricus, 5 days for Clinelymus dahuricus and Cleistogenes squarrosa, 7 days for Arundinella hirta and Pappophorum boreale, and 10 days for Pennisetum alopecuriodes. Compared with the Sheffield region in Britain, the Wulanaodu area of Kerqin Sandyland had a higher proportion of annul grasses with a low germination rate and a longer germination period, and the perennial grasses at the Wulanaodu area had an approximately same germination rate, but a longer germination period. During germination, ruderals showed the potential for risk-sharring, and thus, they had a relatively higher disturbance-resistance capacity.

  20. The chiggerflea Hectopsylla pulex (Siphonaptera: Tungidae as an ectoparasite of free-tailed bats (Chiroptera: Molossidae

    Directory of Open Access Journals (Sweden)

    Júlia Lins Luz

    2009-07-01

    Full Text Available In the present study, we investigated the prevalence and intensity of Hectopsylla pulex infection in Molossus rufus and Molossus molossus, the parasite's choice of attachment site, and whether this host-parasite system varies with host size. Twenty-four bats were captured by hand from the roof of a house in Southeastern Brazil. M. rufus exhibited a prevalence of 71.4% and the mean intensity averaged 5 ectoparasites per bat. M. molossus exhibited a prevalence of 90%, and the average mean intensity was 2.11 ectoparasites. The attachment sites were: ear, tragus, shoulder blade and tibia, anus, wing, axilla, mouth and dactylopatagium. A positive correlation was observed between the bats' weight and the number of fleas.

  1. Usefulness of component resolved analysis of cat allergy in routine clinical practice.

    Science.gov (United States)

    Eder, Katharina; Becker, Sven; San Nicoló, Marion; Berghaus, Alexander; Gröger, Moritz

    2016-01-01

    Cat allergy is of great importance, and its prevalence is increasing worldwide. Cat allergens and house dust mite allergens represent the major indoor allergens; however, they are ubiquitous. Cat sensitization and allergy are known risk factors for rhinitis, bronchial hyperreactivity and asthma. Thus, the diagnosis of sensitization to cats is important for any allergist. 70 patients with positive skin prick tests for cats were retrospectively compared regarding their skin prick test results, as well as their specific immunoglobulin E antibody profiles with regard to their responses to the native cat extract, rFel d 1, nFel d 2 and rFel d 4. 35 patients were allergic to cats, as determined by positive anamnesis and/or nasal provocation with cat allergens, and 35 patients exhibited clinically non-relevant sensitization, as indicated by negative anamnesis and/or a negative nasal allergen challenge. Native cat extract serology testing detected 100% of patients who were allergic to cats but missed eight patients who showed sensitization in the skin prick test and did not have allergic symptoms. The median values of the skin prick test, as well as those of the specific immunoglobulin E antibodies against the native cat extract, were significantly higher for allergic patients than for patients with clinically non-relevant sensitization. Component based diagnostic testing to rFel d 1 was not as reliable. Sensitization to nFel d 2 and rFel d 4 was seen only in individual patients. Extract based diagnostic methods for identifying cat allergy and sensitization, such as the skin prick test and native cat extract serology, remain crucial in routine clinical practice. In our study, component based diagnostic testing could not replace these methods with regard to the detection of sensitization to cats and differentiation between allergy and sensitization without clinical relevance. However, component resolved allergy diagnostic tools have individual implications, and future

  2. Significant improvement of eczema with skin care and food elimination in small children.

    Science.gov (United States)

    Norrman, Gunilla; Tomicić, Sara; Böttcher, Malin Fagerås; Oldaeus, Göran; Strömberg, Leif; Fälth-magnusson, Karin

    2005-10-01

    To evaluate common methods of investigation and treatment in children younger than 2 y of age with eczema, with or without sensitization to food allergens. One hundred and twenty-three children younger than 2 y of age with eczema and suspected food allergy were included in this prospective study. The children underwent skin-prick test with cow's milk, fresh hen's egg white and wheat. Specific IgE to milk and egg white was analysed. The eczema extent and severity was estimated with SCORAD before and after treatment. Children with a positive skin-prick test were instructed to exclude that food item from their diet. All children were treated with emollients and topical steroids when needed. Sixty-two of the children were skin-prick positive to at least one of the allergens; 62% had mild, 30% moderate and 8% severe eczema at their first visit. After treatment, 90% had mild, 10% moderate and 0% severe eczema. Forty-six per cent of the children had circulating IgE antibodies to milk or egg white. Ten per cent had specific IgE but negative skin-prick test to the same allergen. This subgroup improved their eczema significantly without elimination diet. The conventional treatments for children with eczema, i.e. skin care and food elimination, are effective. The beneficial effect of skin care as the first step should not be neglected, and it may not be necessary to eliminate food allergens to relieve skin symptoms in all food-sensitized children with eczema.

  3. Bee pollen: a dangerous food for allergic children. Identification of responsible allergens.

    Science.gov (United States)

    Martín-Muñoz, M F; Bartolome, B; Caminoa, M; Bobolea, I; Ara, M C Garcia; Quirce, S

    2010-01-01

    Bee pollen has been proposed as a food supplement, but it can be a dangerous food for people with allergy. We study an allergic reaction after ingestion of bee pollen in a 4-year-old boy who had developed rhinitis in the last spring and autumn. We performed a prick-by-prick test with bee pollen and skin prick tests with the most important local pollens, house dust mites, common fungi, and animal danders. The levels of serum tryptase, serum total IgE and specific IgE against bee venom and local pollen extracts were determined. The composition of the bee pollen was analysed and SDS-PAGE immunoblotting and blotting-inhibition were carried out. Prick tests were positive to bee pollen and all local pollens extracts and negative to any other allergen sources. The bee pollen sample contained pollens from Quercus genus, and Asteraceae (Compositae) and Rosaceae families. Total IgE was 435 kU/l. Serum specific IgE to bee pollen was 6 kU/l and greater than 0.35 kU/L against pollens from Artemisia vulgaris, Taraxacum officinalis, Cupressus arizonica, Olea europaea, Platanus acerifolia and Lolium perenne as well as to n Art v 1 and other pollen marker allergens. Tryptase level was 3.5 mcg/mL. SDS-PAGE immunoblotting-inhibition points to Asteraceae pollen as the possible cause of the allergic reaction. Foods derived from bees can be dangerous to people with allergy to pollen. Copyright © 2009 SEICAP. Published by Elsevier Espana. All rights reserved.

  4. Pooled HIV-1 viral load testing using dried blood spots to reduce the cost of monitoring antiretroviral treatment in a resource-limited setting.

    Science.gov (United States)

    Pannus, Pieter; Fajardo, Emmanuel; Metcalf, Carol; Coulborn, Rebecca M; Durán, Laura T; Bygrave, Helen; Ellman, Tom; Garone, Daniela; Murowa, Michael; Mwenda, Reuben; Reid, Tony; Preiser, Wolfgang

    2013-10-01

    Rollout of routine HIV-1 viral load monitoring is hampered by high costs and logistical difficulties associated with sample collection and transport. New strategies are needed to overcome these constraints. Dried blood spots from finger pricks have been shown to be more practical than the use of plasma specimens, and pooling strategies using plasma specimens have been demonstrated to be an efficient method to reduce costs. This study found that combination of finger-prick dried blood spots and a pooling strategy is a feasible and efficient option to reduce costs, while maintaining accuracy in the context of a district hospital in Malawi.

  5. Neonatal vitamin A supplementation associated with increased atopy in girls

    DEFF Research Database (Denmark)

    Aage, S; Kiraly, N; Da Costa, K

    2015-01-01

    randomized controlled trial in Guinea-Bissau. METHODS: In 2002-2004, we randomized 4345 normal birthweight neonates to NVAS (50 000 IU retinyl palmitate) or placebo together with their Bacillus Calmette-Guérin vaccination. In 2013, we visited the 1692 (39%) children now aged 8-10 years who were still living...... in the study area, and 1478 (87%) were found at home. Provided consent, a skin prick test was performed, and history of allergic symptoms was recorded. Associations of NVAS and atopy (defined as skin prick test reaction of ≥3 mm) were analysed using binomial regression. RESULTS: Of the 1430 children...

  6. Introduction of the flora, life forms and chorology of plants of some regions in western Chabahar (Sistan and Baluchestan Province

    Directory of Open Access Journals (Sweden)

    Somayeh Salimi

    2014-12-01

    Full Text Available This study focused on Zarabad, Cheraghabad and Nogombecom plants of sub-basin watershed city of Chabahar, Iran. The studied flora included 78 species which belonged to 66 genera and 29 families. Poaceae with 16 genera and 19 species, Asteraceae with 7 genera and 8 species, Chenopodiaceae with 5 genera and 4 species, Papilionaceae, with 5 genera and 6 species, constituted the largest plant families respectively. The largest genera of the region were: Salsola, Heliotropium and Plantago, respectively. 42% of the flora belonged to Nobo-Sindian elements, 35%to Irano-Turanian, 18%were bioregional elements and 5% of the species were cosmopolitan. Flora of the studied area, included therophytes (56%, chamaephytes (15%, phanerophytes (14%, geophytes (12% and hemicryptophytes (3%.

  7. Mensuração da evolução terapêutica com paquímetro digital na Paralisia Facial Periférica de Bell Measurement of evolution therapy using a digital caliper in Palsy Bell

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    Claudia Hosana da Maceno Salvador

    2012-01-01

    Full Text Available OBJETIVO: avaliar o uso do paquímetro digital na mensuração dos movimentos da mímica facial em diferentes momentos do tratamento fonoaudiológico. MÉTODO: estudo longitudinal prospectivo, em 20 sujeitos com idade entre 07 e 70 anos, sendo 13 do genero feminino e 07 masculino, com diagnóstico de paralisia facial periférica de Bell, atendidos no Ambulatório de Paralisia Facial, da disciplina de otorrinolaringologia de um Hospital Público Universitário. Neste estudo foi adotado o uso de um medidor paquímetro digital da marca Digimess 100.174BL, instrumento com resolução de 0,00mm/152,78mm. As medições foram realizadas no movimento da mímica facial, sempre partindo de um ponto fixo para o ponto móvel nas estruturas: tragus e comissura labial, canto externo do olho e comissura labial e também canto interno do olho e asa do nariz, sendo realizadas pré e pós tratamento fonoaudiológico. A quantificação da incompetência do movimento foi mensurada por meio de porcentagem simples. Foi aplicado teste dos Postos Sinalizados de Wilcoxon, para verificar possíveis diferenças entre ambos os momentos considerados (com e sem movimentos, como as variáveis de interesse. RESULTADOS: as mensurações tiveram um resultado estatisticamente significante (pPURPOSE: to assess the use of the digital caliper in the measurement of the facial mimic movements in different moments of the speech therapy. METHOD: prospective longitudinal study, with 20 subjects between 7 and 70 years-old, 13 females and 7 males, all diagnosed with Bell’s Palsy, attended in the Facial Paralysis Ambulatory, of the otorhinolaryngology subject of a University Public Hospital. The use of a Digimess 100,174BL digital measuring caliper was adopted for this study. The measurements were carried out in the facial mimic movement, always starting from a fixed point to a mobile point in the structures: the tragus and the labial commissure, external corner of the eye and labial

  8. Fungal atopy in adult cystic fibrosis.

    LENUS (Irish Health Repository)

    Henry, M

    2012-02-03

    This study set out to estimate the prevalence of atopy to a variety of common ubiquitous fungi, including A. fumigatus, in cystic fibrosis (CF), and to evaluate the investigations by which the diagnosis was made. Particular attention was paid to the usefulness of skin testing and immunoassays in detecting which patients had simple fungal atopy, and which patients were at high risk of developing allergic bronchopulmonary mycoses. This cross-sectional study included 21 adult CF patients and 20 matched controls. Serum samples were taken for the measurement of total serum IgE and specific serum IgE to nine common fungi. Immediate hypersensitivity skin prick testing to each of the fungi was also performed. Simple fungal atopy was described in subjects fulfilling the following criteria: total serum IgE > 100 KU l(-1) with specific radioimmunoassay > or = grade 1 to at least one fungus and a positive skin prick test (SPT) > or = 3 mm to the same fungus. \\'High risk\\' for developing allergic bronchopulmonary mycosis (ABPM) was described in subjects fulfilling the following criteria: total serum IgE > 200 KU l(-1) with specific radioimmunoassay > or = grade 2 to at least one fungus and a positive skin prick test (SPT) > or = 6 mm to the same fungus. The adult CF group had a significantly higher total SPT score (P=0.005) and mean total serum IgE (P<0.05) than controls. Forty-three percent of CF patients fulfilled the criteria for fungal atopy to at least a single fungus. Over half this group had an atopic tendency to more than one fungus. Nineteen percent of the CF group were at least \\'high risk\\' of developing ABPM. Skin prick testing is a better marker of fungal atopy and a better predictor of those adult CF patients at higher risk of developing ABPM than specific radioimmunoassay serum testing. There is a high prevalence of fungal atopy in the adult CF population. Total serum IgE and skin prick testing are good predictors of fungal atopy and help predict those at

  9. Association between allergies and risk of pancreatic cancer.

    Science.gov (United States)

    Cotterchio, Michelle; Lowcock, Elizabeth; Hudson, Thomas J; Greenwood, Celia; Gallinger, Steven

    2014-03-01

    Less than 10% of pancreatic cancer cases survive 5 years, yet its etiology is not well understood. Studies suggest allergies are associated with reduced pancreatic cancer risk. Our study collected additional information on allergies (including skin prick test results and differentiation of allergic/nonallergic asthma), and is the first to assess possible confounding by allergy medications. A population-based case-control study was designed to comprehensively assess the association between allergy and pancreatic cancer risk. Pancreas cancer cases were diagnosed during 2011 to 2012, and identified through the Ontario Cancer Registry (345 cases). Population-based controls were identified using random digit dialing and age/sex frequency matched to cases (1,285 controls). Questionnaires collected lifetime allergy history (type of allergy, age at onset, skin prick testing results), allergy medications, and established pancreas cancer risk factors. Logistic regression was used to estimate odd ratios and test potential confounders, including allergy medications. Hay fever was associated with a significant reduction in pancreatic cancer risk [AOR = 0.68; 95% confidence intervals (CI), 0.52-0.89], and reduction was greatest for those whose skin prick test was positive for hay fever allergens. No particular patterns were observed as regards age at onset and duration of allergy. Positive dust/mold allergy skin prick test and animal allergies were associated with a statistically significant reduced pancreatic cancer risk; AOR = 0.49; 95% CI, 0.31-0.78 and AOR = 0.68; 95% CI, 0.46-0.99, respectively. Asthma was not associated with pancreatic cancer risk. These findings support the growing body of evidence that suggests certain allergies are associated with reduced pancreatic cancer risk. ©2014 AACR.

  10. Highly accurate prediction of food challenge outcome using routinely available clinical data.

    Science.gov (United States)

    DunnGalvin, Audrey; Daly, Deirdre; Cullinane, Claire; Stenke, Emily; Keeton, Diane; Erlewyn-Lajeunesse, Mich; Roberts, Graham C; Lucas, Jane; Hourihane, Jonathan O'B

    2011-03-01

    Serum specific IgE or skin prick tests are less useful at levels below accepted decision points. We sought to develop and validate a model to predict food challenge outcome by using routinely collected data in a diverse sample of children considered suitable for food challenge. The proto-algorithm was generated by using a limited data set from 1 service (phase 1). We retrospectively applied, evaluated, and modified the initial model by using an extended data set in another center (phase 2). Finally, we prospectively validated the model in a blind study in a further group of children undergoing food challenge for peanut, milk, or egg in the second center (phase 3). Allergen-specific models were developed for peanut, egg, and milk. Phase 1 (N = 429) identified 5 clinical factors associated with diagnosis of food allergy by food challenge. In phase 2 (N = 289), we examined the predictive ability of 6 clinical factors: skin prick test, serum specific IgE, total IgE minus serum specific IgE, symptoms, sex, and age. In phase 3 (N = 70), 97% of cases were accurately predicted as positive and 94% as negative. Our model showed an advantage in clinical prediction compared with serum specific IgE only, skin prick test only, and serum specific IgE and skin prick test (92% accuracy vs 57%, and 81%, respectively). Our findings have implications for the improved delivery of food allergy-related health care, enhanced food allergy-related quality of life, and economized use of health service resources by decreasing the number of food challenges performed. Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

  11. Microbial load and stability of some phyto chemical compounds of selected Sudanese medicinal plant materials as affected by gamma irradiation

    International Nuclear Information System (INIS)

    Musa, H. A. A.; Ahmed, E. E. A.; Osman, G. A. M.; Ali, H. A.; Muller, J. L.

    2011-01-01

    The aim of the present study was to evaluate the effect of gamma irradiation treatment on seeds of pepper cress (lepidium sativum L), seeds of black mustard (brassica nigra L.Koch), leaves of lemon grass (cymbopogon citratus), and calyces of roselle (hibiscus sabdariffa L), pods of senna (cassia senna L) and pods prickly acacia (Acacia nilotica L.). The radiation processing was carried out at dose levels of 0, 5, 10. 15 kGy. The irradiated and control samples were analyzed for microbial load, tannins and total phenol content as well as DPPH scavenging activity. The results indicated that gamma radiation treatment significantly reduced microbial load and showed that the total microbial load decreased linearly with absorbed radiation dose. They, also, indicated maximum reduction in tannin content in lemon grass, prickly acacia and roselle. On the other hand, irradiation with 15 kGy increased the tannin and phenol contents in black mustard, pepper cress and senna and reduced the phenol content of roselle and prickly acacia. The results also revealed that gamma irradiation resulted in significant decrease of DPPH radical scavenging activity of the different studied methanolic extracts with exception of pepper cress seeds.(Author)

  12. An evaluation of the diagnostic value of different skin tests with egg in clinically egg-allergic children having atopic dermatitis

    DEFF Research Database (Denmark)

    Hansen, Tine K; Høst, Arne; Bindslev-Jensen, Carsten

    2004-01-01

    Skin testing is a common diagnostic procedure in food allergy, but the final diagnosis of food allergy is based on the clinical response to food challenge. We studied the value of the skin prick-prick test (SPT), skin application food test (SAFT) and atopy patch test (APT) with fresh egg extract......-allergic children were positive in SPT and 40-60% in APT. In APT and in SPT false-positive reactions to egg were observed. In this study comprising a small number of patients including control subjects, neither SAFT nor APT with fresh whole egg extract were able to increase the diagnostic accuracy in detecting egg...

  13. Birth order modifies the effect of IL13 gene polymorphisms on serum IgE at age 10 and skin prick test at ages 4, 10 and 18: a prospective birth cohort study

    Science.gov (United States)

    2010-01-01

    Background Susceptibility to atopy originates from effects of the environment on genes. Birth order has been identified as a risk factor for atopy and evidence for some candidate genes has been accumulated; however no study has yet assessed a birth order-gene interaction. Objective To investigate the interaction of IL13 polymorphisms with birth order on allergic sensitization at ages 4, 10 and 18 years. Methods Mother-infant dyads were recruited antenatally and followed prospectively to age 18 years. Questionnaire data (at birth, age 4, 10, 18); skin prick test (SPT) at ages 4, 10, 18; total serum IgE and specific inhalant screen at age 10; and genotyping for IL13 were collected. Three SNPs were selected from IL13: rs20541 (exon 4, nonsynonymous SNP), rs1800925 (promoter region) and rs2066960 (intron 1). Analysis included multivariable log-linear regression analyses using repeated measurements to estimate prevalence ratios (PRs). Results Of the 1456 participants, birth order information was available for 83.2% (1212/1456); SPT was performed on 67.4% at age 4, 71.2% at age 10 and 58.0% at age 18. The prevalence of atopy (sensitization to one or more food or aeroallergens) increased from 19.7% at age 4, to 26.7% at 10 and 41.1% at age 18. Repeated measurement analysis indicated interaction between rs20541 and birth order on SPT. The stratified analyses demonstrated that the effect of IL13 on SPT was restricted only to first-born children (p = 0.007; adjusted PR = 1.35; 95%CI = 1.09, 1.69). Similar findings were noted for firstborns regarding elevated total serum IgE at age 10 (p = 0.007; PR = 1.73; 1.16, 2.57) and specific inhalant screen (p = 0.034; PR = 1.48; 1.03, 2.13). Conclusions This is the first study to show an interaction between birth order and IL13 polymorphisms on allergic sensitization. Future functional genetic research need to determine whether or not birth order is related to altered expression and methylation of the IL13 gene. PMID:20403202

  14. Birth order modifies the effect of IL13 gene polymorphisms on serum IgE at age 10 and skin prick test at ages 4, 10 and 18: a prospective birth cohort study

    Directory of Open Access Journals (Sweden)

    Ogbuanu Ikechukwu U

    2010-04-01

    Full Text Available Abstract Background Susceptibility to atopy originates from effects of the environment on genes. Birth order has been identified as a risk factor for atopy and evidence for some candidate genes has been accumulated; however no study has yet assessed a birth order-gene interaction. Objective To investigate the interaction of IL13 polymorphisms with birth order on allergic sensitization at ages 4, 10 and 18 years. Methods Mother-infant dyads were recruited antenatally and followed prospectively to age 18 years. Questionnaire data (at birth, age 4, 10, 18; skin prick test (SPT at ages 4, 10, 18; total serum IgE and specific inhalant screen at age 10; and genotyping for IL13 were collected. Three SNPs were selected from IL13: rs20541 (exon 4, nonsynonymous SNP, rs1800925 (promoter region and rs2066960 (intron 1. Analysis included multivariable log-linear regression analyses using repeated measurements to estimate prevalence ratios (PRs. Results Of the 1456 participants, birth order information was available for 83.2% (1212/1456; SPT was performed on 67.4% at age 4, 71.2% at age 10 and 58.0% at age 18. The prevalence of atopy (sensitization to one or more food or aeroallergens increased from 19.7% at age 4, to 26.7% at 10 and 41.1% at age 18. Repeated measurement analysis indicated interaction between rs20541 and birth order on SPT. The stratified analyses demonstrated that the effect of IL13 on SPT was restricted only to first-born children (p = 0.007; adjusted PR = 1.35; 95%CI = 1.09, 1.69. Similar findings were noted for firstborns regarding elevated total serum IgE at age 10 (p = 0.007; PR = 1.73; 1.16, 2.57 and specific inhalant screen (p = 0.034; PR = 1.48; 1.03, 2.13. Conclusions This is the first study to show an interaction between birth order and IL13 polymorphisms on allergic sensitization. Future functional genetic research need to determine whether or not birth order is related to altered expression and methylation of the IL13 gene.

  15. 78 FR 40669 - Endangered and Threatened Wildlife and Plants; Endangered Species Status for Cape Sable...

    Science.gov (United States)

    2013-07-08

    ... and Plants; Endangered Species Status for Cape Sable Thoroughwort, Florida Semaphore Cactus, and... thoroughwort), Consolea corallicola (Florida semaphore cactus), and Harrisia aboriginum (aboriginal prickly...

  16. The use of halophytic plants for salt phytoremediation in constructed wetlands.

    Science.gov (United States)

    Farzi, Abolfazl; Borghei, Seyed Mehdi; Vossoughi, Manouchehr

    2017-07-03

    This research studied the use of constructed wetlands (CWs) to reduce water salinity. For this purpose, three halophytic species of the Chenopodiaceae family (Salicornia europaea, Salsola crassa, and Bienertia cycloptera) that are resistant to saline conditions were planted in the CWs, and experiments were conducted at three different salinity levels [electrical conductivity (EC)∼2, 6, 10 dS/m]. EC and concentrations of calcium (Ca), magnesium (Mg), sodium (Na), and chlorine (Cl) were measured before and after phytoremediation with a retention time of 1 week. The results suggested that these plants were able to grow well and complete their life cycles at all the salinity levels within this study. Moreover, these plants reduced the measured parameters to acceptable levels. Therefore, these plants can be considered good options for salt phytoremediation.

  17. Mensuração da evolução terapêutica com paquímetro digital na Paralisia Facial Periférica de Bell

    Directory of Open Access Journals (Sweden)

    Claudia Hosana da Maceno Salvador

    2013-06-01

    Full Text Available OBJETIVO: avaliar o uso do paquímetro digital na mensuração dos movimentos da mímica facial em diferentes momentos do tratamento fonoaudiológico. MÉTODO: estudo longitudinal prospectivo, em 20 sujeitos com idade entre 07 e 70 anos, sendo 13 do genero feminino e 07 masculino, com diagnóstico de paralisia facial periférica de Bell, atendidos no Ambulatório de Paralisia Facial, da disciplina de otorrinolaringologia de um Hospital Público Universitário. Neste estudo foi adotado o uso de um medidor paquímetro digital da marca Digimess 100.174BL, instrumento com resolução de 0,00mm/152,78mm. As medições foram realizadas no movimento da mímica facial, sempre partindo de um ponto fixo para o ponto móvel nas estruturas: tragus e comissura labial, canto externo do olho e comissura labial e também canto interno do olho e asa do nariz, sendo realizadas pré e pós tratamento fonoaudiológico. A quantificação da incompetência do movimento foi mensurada por meio de porcentagem simples. Foi aplicado teste dos Postos Sinalizados de Wilcoxon, para verificar possíveis diferenças entre ambos os momentos considerados (com e sem movimentos, como as variáveis de interesse. RESULTADOS: as mensurações tiveram um resultado estatisticamente significante (p<0,05 em todas as estruturas medidas propostas (tragus e comissura labial, canto externo do olho e comissura labial e canto interno do olho e asa do nariz, demonstrando que há possibilidades de se fazer medições de movimento e de ausência de movimento utilizando o paquímetro digital. CONCLUSÃO: o paquímetro mostrou ser um instrumento útil que permitiu comparar, de forma objetiva, a evolução da reabilitação da mímica facial na Paralisia Facial Periférica de Bell na amostra estudada.

  18. Congenital hairy polyp of the oropharynx presenting as an esophageal mass in a neonate, a case report and literature review.

    Science.gov (United States)

    Richter, Amy; Mysore, Krupa; Schady, Deb; Chandy, Binoy

    2016-01-01

    To review the literature of congenital hairy polyps and describe the clinical presentation, operative management, and histologic findings of a congenital hairy polyp arising from the palatopharyngeus muscle in a neonate with recurrent choking episodes. Chart review of a 2-month-old male referred to a tertiary care pediatric hospital. We present a case of a 2-month-old male who presented to the emergency room with recurrent episodes of choking and vomiting. The patient was previously healthy with no prior medical or neonatal history. The parents noted a small fleshy mass in the patient's oropharynx that he would chew on and swallow after several minutes. However, on physical exam, there was no evidence of oropharyngeal mass. The patient did not have respiratory distress. Imaging revealed a 22×7×11mm oblong, fatty mass in the lower cervical and upper thoracic esophagus with a thin stalk extending proximally to the upper collapsed esophagus. Intraoperative recorded laryngoscopy revealed a pedunculated soft palate mass attached to the right superior palatopharyngeus muscle. Histopathology revealed ectodermal and mesodermal elements in a polypoid structure lined by keratinizing squamous epithelium with adnexal structures and central mature adipose tissue, consistent with congenital hairy polyp resembling an accessory tragus of the ear and branchial anomaly. At 6-week follow up, the patient was doing well and gaining weight appropriately with no further choking episodes. There was no evidence of velopharyngeal dysfunction on follow up exam. The surgical site was completely healed and there was no evidence of recurrence. Congenital hairy polyps of the naso- and oropharynx are rare but may present as airway or esophageal masses, causing respiratory distress or choking episodes in a pediatric patient. The pathologic findings of keratinizing squamous epithelium, adnexal structures, adipose and cartilage tissues resemble congenital accessory tragus and may be considered a

  19. [Profile of sensitization to allergens in children with atopic dermatitis assisting to Allergology Service of University Hospital, Nuevo Leon, Mexico].

    Science.gov (United States)

    Yong-Rodríguez, Adrián; Macías-Weinmann, Alejandra; Palma-Gómez, Samuel; Arias-Cruz, Alfredo; Pérez-Vanzzini, Rafael; Gutiérrez-Mujica, José Julio; González-Díaz, Sandra Nora

    2015-01-01

    Sensitization to allergens in atopic dermatitis patients is a risk factor for developing asthma and allergic rhinitis in the future,as well as an aggravating factor in the course of the disease. Recent studies have attributed the activity of the proteases of some antigens to cause a grater defect in the epithelial barrier and a more severe disease. To know the sensitization to allergens pattern in children with atopic dermatitis attended at Allergology Service of University Hospital of UANL, Mexico, and to know if these children have higher sensitization to antigens with proteolytic activity. A retrospective study was done reviewing the skin prick test reports done in our service to children ranging from 5 months to 16 years old, diagnosed with atopic dermatitis during a period of 2 years, from January 2012 to January 2014. The frequency of sensitization to aeroallergens and food were analyzed as well as the weal size (≥6mm) on the skin in response to each particular allergen in the case of food skin prick test. Reports of skin tests of 66 children, 30 boys and 36 girls, were included; 37 of children were sensitized to more than one allergen,18/66 had asthma and/or allergic rhinitis, 40/66 60% skin prick tests were positive to high activity protease aeroallergens (Dermatophagoides pteronyssinus/Dermatophagoides farinae). Regarding food, sensitization was seen in 38 children; fruits and vegetables were the two most common foods. Only seven children had skin prick weal bigger than 6 mm, mainly to egg, fish and cow's milk. Children with atopic dermatitis are often sensitized to high protease activity aeroallergens, polysensitization is very common and the association with airway allergy is seen early in life. Sensitization to food is also common in these patients, but only a small percentage showed a response large enough to be associated with disease severity.

  20. Freshly squeezed: anaphylaxis caused by drone larvae juice.

    Science.gov (United States)

    Stoevesandt, J; Trautmann, A

    2017-11-30

    Drone larvae are mostly considered a by-product of beekeeping, but have recently been advo-cated as a high-protein source of food. There are as yet no data concerning their allergenic po-tential. We report on a 29-year old bee keeper who experienced an anaphylactic reaction following the consumption of a freshly prepared beverage from raw drone larvae. Larvae-specific sensitization was confirmed by prick-to-prick and basophil activation testing. Bee stings and classical bee products including honey and royal jelly were tolerated. This is the hitherto first report on IgE-mediated allergy to drone larvae. We suggest that a certain awareness towards the allergenicity of bee larvae is required.

  1. Asthma cases in childhood attributed to atopy in tropical area in Brazil.

    Science.gov (United States)

    Souza da Cunha, Sergio; Barreto, Mauricio Lima; Fiaccone, Rosemeire Leovigildo; Cooper, Philip J; Alcantara-Neves, Neuza Maria; Simões, Silvia de Magalhães; Cruz, Alvaro Augusto; Rodrigues, Laura Cunha

    2010-12-01

    This study aimed to explore the association between asthma and atopy in a cohort of children living in a large urban center in Brazil. Atopy was defined by the presence of allergen-specific IgE in serum or by a positive skin prick test. In a sample of 1 445 Brazilian children, the association between the prevalence of asthma, skin prick test positivity, and allergen-specific IgE in serum was investigated. The prevalence of asthma was 22.6%. The presence of serum allergen-specific IgE was frequent in asthmatics and nonasthmatics, and the prevalence of asthma increased only with levels of allergen-specific IgE > 3.5 kilounits/L. The proportion of asthma attributable to atopy was estimated to be 24.5% when atopy was defined by the presence of allergen-specific IgE. With a given level of specific IgE, no association between skin test reactivity and asthma was observed. Skin prick tests were less sensitive than specific IgE for detection of atopy. Most asthma cases in an urban underprivileged setting in Brazil were not attributable to atopy. This observation has important implications for understanding the risk factors for the asthma epidemic in Latin America.

  2. Immediate Hypersensitivity Reactions Induced by Triamcinolone in a Patient with Atopic Dermatitis.

    Science.gov (United States)

    Son, Jee Hee; Park, Sook Young; Cho, Yong Se; Chung, Bo Young; Kim, Hye One; Park, Chun Wook

    2018-03-19

    Corticosteroids are potent anti-inflammatory and anti-allergic agents used in the treatment of various inflammatory diseases, including allergic disease. They are frequently considered the therapy-of-choice for many skin diseases. However, allergic reactions caused by corticosteroids have been reported. Among these, delayed reactions to topical steroids are more common, whereas immediate reactions to systemic steroids are rare. Herein, we report the case of a 32-year-old woman with triamcinolone-induced immediate hypersensitivity reaction, in which the patient had a positive prick test result with triamcinolone. She has had atopic dermatitis (AD) for three years. She had used systemic steroid, cyclosporine, and antihistamine with topical steroids for AD. In clinic, approximately 10 minutes after intralesional injection of triamcinolone, she complained of erythematous patches with slight elevation and itching on the face, trunk, and both hands. After intravenous injection of dexamethasone, her symptoms got worse. After treatment with epinephrine, all symptoms resolved within two hours. We performed an open test and skin prick test. She had a positive result only from the prick test with triamcinolone; all other steroids showed negative results from the open tests. Dermatologists should be aware of the possibility of anaphylaxis or other allergic hypersensitivity in response to corticosteroids. © 2018 The Korean Academy of Medical Sciences.

  3. Oral desensitization to milk: how to choose the starting dose!

    Science.gov (United States)

    Mori, Francesca; Pucci, Neri; Rossi, Maria Elisabetta; de Martino, Maurizio; Azzari, Chiara; Novembre, Elio

    2010-01-01

    Mori F, Pucci N, Rossi ME, de Martino M, Azzari C, Novembre E. Oral desensitization to milk: how to choose the starting dose! Pediatr Allergy Immunol 2010: 21: e450–e453. © 2009 John Wiley & Sons A/S A renewed interest in oral desensitization as treatment for food allergy has been observed in the last few years. We studied a novel method based on the end point skin prick test procedure to establish the starting dose for oral desensitization in a group of 30 children higly allergic to milk. The results (in terms of reactions to the first dose administered) were compared with a group of 20 children allergic to milk as well. Such control group started to swallow the same dose of 0.015 mg/ml of milk. None reacted to the first dose when administered according to the end point skin prick test. On the other side, ten out of 20 children (50%) from the control group showed mild allergic reactions to the first dose of milk. In conclusion the end point skin prick test procedure results safe and easy to be performed in each single child in order to find out the starting dose for oral desensitization to milk, also by taking into account the individual variability. PMID:19624618

  4. Allergy to iguana.

    Science.gov (United States)

    Kelso, J M; Fox, R W; Jones, R T; Yunginger, J W

    2000-08-01

    Furry animals produce allergens that can cause allergic rhinitis and asthma. In contrast, scaly animals, such as lizards, are assumed not to be allergenic. We sought to evaluate a 32-year-old man who complained of allergic rhinitis and asthma symptoms that occurred exclusively in his own home. He had dogs and cats at home but denied any increase in symptoms specifically associated with these pets. Skin prick testing initially performed to 42 common aeroallergens, including cat, dog, and house dust mite, elicited negative results. He later reported that the symptoms were worse on exposure to his pet iguanas. Skin prick tests were subsequently performed to an extract made from scales from his pet iguana. Extracts were also prepared from several zoo reptiles. Immunoassays for IgE antibody, as well as IgE immunoblots, were performed by using these extracts and the patient's serum. The skin prick test result with the pet iguana scale extract was positive. The patient's serum contained IgE antibody to his own pet iguana and to a zoo iguana. Our patient's history, skin test results, and in vitro studies clearly demonstrate that he is allergic to iguana. Physicians should be aware that such allergy to scaly pets may occur and should not restrict history taking to questions about furry pets.

  5. 78 FR 40136 - Metaldehyde; Amendment To Terminate Uses

    Science.gov (United States)

    2013-07-03

    ..., ornamentals, prickly pear cactus, tomato, strawberry, watercress, and use sites with directions for use in... grown for seed, strawberries, blueberries, and caneberries and bushberries in the former commodity...

  6. Skin Prick Tests and Dose Response Relationship between ...

    African Journals Online (AJOL)

    Subjects and Methods: The study was carried out on 60 bakers working at different bakeries at Dekernis, Dakahlia Governorate, Egypt; and 30 control subjects. All participants were submitted to a self administered questionnaire on respiratory symptoms, general medical examination, chest examination; spirometry and SPT.

  7. "But You Look So Good!": Managing Specific Issues

    Science.gov (United States)

    ... to see include fatigue, pain, cognitive problems like memory loss or trouble solving problems, weakness, blurred vision, numbness, prickly or tingling sensations, heat sensitivity, dizziness, balance/coordination problems, and bladder ...

  8. Comparative availability of cesium and strontium for plant absorption from amended Rupert surface soil and associated subsoil: influence of growth conditions

    International Nuclear Information System (INIS)

    Cataldo, D.A.

    1979-03-01

    Studies were undertaken to determine the plant availability of 134 Cs and 85 Sr amended to Rupert surface soil and an associated subsoil. Concentration ratios for cheatgrass (Bromus tectorum) and tumbleweed (Salsola kali) grown on 134 Cs amended Rupert soil were 0.15 and 0.28, respectively; values for amended subsoils were 0.074 and 0.13, respectively. Rupert surface soil and subsoil amended with 85 Sr gave concentration ratios of 15 and 7, respectively, for both tumbleweed and cheatgrass. While pot size (1 vs 4 kg) had a market effect on concentration ratios, values for greenhouses and growth chamber grown plants were generally similar. Aging of both Rupert surface soil and subsoils for 1 to 30 days prior to planting had a pronounced effect on the availability of 134 Cs for uptake by plants, but no effect on 85 Sr uptake

  9. [Determination of amitrole in agricultural products by high performance liquid chromatography-tandem mass spectrometry].

    Science.gov (United States)

    Li, Li; Fu, Jian; Gao, Hongliang; Ren, Haitao; Lou, Xishan; Guan, Lihui

    2010-03-01

    A high performance liquid chromatography-tandem mass spectrometry method (HPLC-MS/MS) was developed for the analysis of amitrole residues in agricultural products. The samples were extracted by 25% acetone for wheat, fish, pork and liver samples, 1% acetic acid-25% acetone for maize and peanut samples, 1% acetic acid solution for honeysuckle, the powder of ginger, the powder of bunge prickly ash and tea leaves samples, 1% acetic acid solution-dichloromethane for apple, pineapple, spinach, carrot, perilla leaves samples, respectively, followed by liquid-liquid extraction with dichloromethane. The samples were then cleaned up by PCX or Envi-Carb solid-phase extraction cartridge. The amitrole was determined and confirmed by HPLC-MS/MS. The results showed a linear relationship in the range of 0.005 -0.1 mg/kg for amitrole. The correlation coefficient was 0.999 7. The average recoveries of amitrole in wheat, maize, peanut, pineapple, apple, carrot, spinach, pork, the powder of ginger, the powder of bunge prickly ash, perilla, liver, fish, honeysuckle and tea were 67.5% - 98.1%. The relative standard deviations (RSDs) were 1.0% - 9.8%. The limits of quantitation were 10 microg/kg for wheat, maize, peanut, pineapple, apple, carrot, spinach, pork, perilla, liver, fish, honeysuckle and 20 microg/kg for the powder of bunge prickly ash, the powder of ginger and tea, respectively. The method is simple, sensitive and accurate.

  10. A cephalometric study to determine the plane of occlusion in completely edentulous patients.

    Science.gov (United States)

    Hindocha, Amit D; Vartak, Vikas N; Bhandari, Aruna J; Dudani, Mohit T

    2013-01-01

    Determination of the plane of occlusion in completely edentulous patients with the help of the ala-tragus line (Camper's plane) may be questioned. An attempt to devise an alternative method to determine the orientation of the plane of occlusion was made. Cephalometric analysis was used to identify whether a correlation exists between the plane of occlusion of dentulous Indian individuals and other stable cranial landmarks. A negative correlation was found to exist between the occlusal Plane-FH plane angle and the porion-nasion-anterior nasal spine (PoNANS) angle. From the derived mathematical correlation, it was concluded that the angulation of the occlusal plane in completely edentulous subjects may be determined by taking a cephalogram at the diagnostic stage. Further, the clinical applicability of the derived mathematical formula (while determining the plane of occlusion) was tested on completely edentulous patients.

  11. Plasma Proteome Profiling to Assess Human Health and Disease

    DEFF Research Database (Denmark)

    Geyer, Philipp E; Kulak, Nils A; Pichler, Garwin

    2016-01-01

    from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV

  12. Establishment of callus from Opuntia robusta Wendl., a wild and ...

    African Journals Online (AJOL)

    coco santos

    2013-04-22

    Apr 22, 2013 ... plants produce tender cladodes, consumed as vegetable and fruits (prickly pear) .... The highest callus amount was obtained in media supplemented ... CO2 during carboxylation and decarboxylation reactions, improves the ...

  13. Clinical cross-reactivity among foods of the Rosaceae family.

    Science.gov (United States)

    Rodriguez, J; Crespo, J F; Lopez-Rubio, A; De La Cruz-Bertolo, J; Ferrando-Vivas, P; Vives, R; Daroca, P

    2000-07-01

    Foods from the Rosaceae botanical family have been increasingly reported as causes of allergic reaction. Patients frequently have positive skin tests or radioallergosorbent test results for multiple members of this botanical family. Our purpose was to investigate the clinical cross-reactivity assessed by double-blind, placebo-controlled food challenge (DBPCFC) of Rosaceae foods (apricot, almond, plum, strawberry, apple, peach, and pear). Thirty-four consecutive adult patients complaining of adverse reactions to Rosaceae were included in the study. Skin prick tests and CAP System (FEIA) were performed with Rosaceae foods in all patients. Clinical reactivity to Rosaceae was systematically evaluated by open food challenges (OFCs), unless there was a convincing history of a recent severe anaphylaxis. Positive reactions on OFCs were subsequently evaluated by DBPCFCs. Twenty-six and 24 patients had positive skin prick tests and CAP FEIA with Rosaceae, respectively; from these 88% and 100% had positive tests with >/=2. No evidence of clinical reactivity was found in 66% percent of positive skin prick tests and 63% of positive specific IgE determinations to fruits. A total of 226 food challenges (including OFC and DBPCFC) were performed in the 28 patients with positive skin prick tests or CAP System FEIA. Of 182 initial OFCs carried out, 26 (14%) reactions were confirmed by DBPCFCs. Overall, 40 reactions were considered positive in 22 patients with positive skin tests or CAP FEIA. Thirty-eight reactions had been previously reported, the remaining two were detected by systematic challenges. Most reactions were caused by peach (22 patients), apple (6), and apricot (5). Ten patients (46%) were clinically allergic to peach and other Rosaceae. Positive skin test and CAP System FEIA should not be taken as the only guide for multi-species dietary restrictions. Nevertheless, the potential clinical allergy to other Rosaceae should not be neglected. If the reported reaction is

  14. Obstructive sleep apnoea and atopy among middle aged chronic obstructive pulmonary disease and bronchial asthma patients.

    Science.gov (United States)

    Kumar, Raj; Nagar, Devender; Mallick, Adeeb; Kumar, Manoj; Tarke, Chandrakant R; Goel, Nitin

    2013-09-01

    Obstructive sleep apnoea syndrome is associated with significant morbidity. A high prevalence of obstructive sleep apnoea (OSA) symptoms has been reported in patients with asthma and chronic obstructive pulmonary disease (COPD). There are limited studies regarding relationship between atopy and OSA. To study the risk of obstructive sleep apnoea among middle aged chronic obstructive pulmonary disease and asthma patients by a home based sleep study and its association with atopy. Patients with asthma and COPD were evaluated for OSA symptoms by Epworth sleepiness scale (ESS) and Berlin questionnaire (BQ). ESS score > or = 9 was considered as high risk for OSA. Patients having high risk for OSA by ESS and BQ were further evaluated for OSA by home based sleep study. Skin prick test against common allergens was done to diagnose atopy in these patients. Among 400 patients (229, 57.25% male and 171, 42.75% female) 328 were asthmatics and 72 were COPD patients. ESS and BQ was positive in 11.25% (45/400) and 18.25% (73/400) patients respectively. ESS was positive in 10.67% (35/328) of asthma and 13.88% (10/72) of COPD patients. BQ was positive in 18.29% (60/328) of asthmatic and 18.05% (13/72) of COPD patients. Skin prick test was positive in 74.16% patients. The maximum positivity was found in asthmatics (139/155, 89.68%) compared to COPD patients (16/155, 10.32%). Skin prick test was done for 40 patients out of 73 of Asthma and COPD patients who were found positive by ESS and BQ. 72.5% patients were found to be atopic. Out of 19 patients in whom home polysomnography was done, 13 patients consented for skin prick test with common aeroallergens and 9 (69.23%) patients were found to be atopic. There is an increased risk of obstructive sleep apnoea among middle aged chronic obstructive pulmonary disease and asthma patients. Atopy could be associated with OSA. The association needs to be proved in a larger study.

  15. analysis of genetic diversity and relationship of Chinese Rosa rugosa

    African Journals Online (AJOL)

    Administrator

    2011-05-30

    May 30, 2011 ... Rosa rugosa is a deciduous shrub of the genus Rosa, having prickly stems ... eastern Shandong province and Tumen River estuary ..... Smithsonian Institution, Washington DC, USA. Sokolov SY ... Distribution of trees and.

  16. Prescription for herbal healing

    National Research Council Canada - National Science Library

    Balch, Phyllis A; Bell, Stacey J

    2012-01-01

    .... John's Wort, to less familiar remedies, such as khella and prickly ash Chinese and ayurvedic herbal combinations Discussion of more than 150 common disorders from acne to yeast infection, and suggested herbal treatment therapies"--

  17. Anti-phenolic glycolipid-I (PGL-I determination using blood collection on filter paper in leprosy patients

    Directory of Open Access Journals (Sweden)

    TOMIMORI-YAMASHITA Jane

    1999-01-01

    Full Text Available The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS containing 0.5% gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals.

  18. Prevalence of Wheat Allergy in Japanese Adults

    Directory of Open Access Journals (Sweden)

    Eishin Morita

    2012-01-01

    Conclusions: The prevalence of wheat allergy in Japanese adults was found to be 0.21% by using a combination of questionnaire-based examination, skin prick test and serum omega-5 gliadin-specific IgE test.

  19. Allergic Colitis in Infants Related to Cow’s Milk: Clinical Characteristics, Pathologic Changes, and Immunologic Findings

    Directory of Open Access Journals (Sweden)

    Man-Chun Yu

    2013-02-01

    Conclusion: Endoscopic biopsy specimens of intestine confirm the diagnosis of AC. However, allergen skin prick test and IgE antibody to milk protein components also provide helpful diagnostic tools for this rare disease in children.

  20. Cactus (Opuntia ficus indica f. inermis) fruit juice protects against ...

    African Journals Online (AJOL)

    HICHEM

    2013-12-18

    Dec 18, 2013 ... A putative beneficial effect of Opuntia ficus indica f. inermis prickly pear ... peroxidation levels were also increased in animals given ethanol compared to the controls. ..... mechanisms are suggested in this study to explain the.

  1. Thyroid Scan and Uptake

    Medline Plus

    Full Text Available ... painless. However, during the thyroid scan, you may feel uncomfortable when lying completely still with your head ... When the radiotracer is given intravenously, you will feel a slight pin prick when the needle is ...

  2. Foreign Body Retrieval

    Medline Plus

    Full Text Available ... experience during and after the procedure? You will feel a slight pin prick when the needle is ... which is numbed using local anesthetic. You may feel pressure when the catheter is inserted into the ...

  3. Children's (Pediatric) CT (Computed Tomography)

    Medline Plus

    Full Text Available ... intravenous contrast material is used, your child will feel a slight pin prick when the IV is ... reviewed on March 20, 2017 Send us your feedback Did you find the information you were looking ...

  4. Transjugular Intrahepatic Portosystemic Shunt (TIPS)

    Medline Plus

    Full Text Available ... general anesthesia is that the patient will not feel anything. You will be positioned on your back. ... will be attached to your body. You will feel a slight pin prick when the needle is ...

  5. Computed Tomography (CT) -- Sinuses

    Medline Plus

    Full Text Available ... an intravenous contrast material is used, you will feel a pin prick when the needle is inserted ... reviewed on March 30, 2018 Send us your feedback Did you find the information you were looking ...

  6. Abdominal and Pelvic CT

    Medline Plus

    Full Text Available ... an intravenous contrast material is used, you will feel a pin prick when the needle is inserted ... reviewed on June 16, 2016 Send us your feedback Did you find the information you were looking ...

  7. Children's (Pediatric) Nuclear Medicine

    Medline Plus

    Full Text Available ... the radiotracer is given intravenously, your child will feel a pin prick, much like a shot, when ... or no taste. If inhaled, your child should feel no differently than when breathing room air or ...

  8. Computed Tomography (CT) -- Head

    Medline Plus

    Full Text Available ... an intravenous contrast material is used, you will feel a pin prick when the needle is inserted ... reviewed on June 08, 2016 Send us your feedback Did you find the information you were looking ...

  9. Catheter Angiography

    Medline Plus

    Full Text Available ... or extremities in patients after trauma. evaluate arteries feeding a tumor prior to surgery or other procedures ... be asked to empty your bladder. You will feel a slight pin prick when the needle is ...

  10. Single and multiple food allergies in infants with proctocolitis.

    Science.gov (United States)

    Koksal, B T; Barıs, Z; Ozcay, F; Yilmaz Ozbek, O

    Food protein-induced allergic proctocolitis is a frequent cause of rectal bleeding in infants. Characteristics of infants with multiple food allergies have not been defined. This study aimed to identify characteristics of infants with proctocolitis and compare infants with single and multiple food allergies. A total of 132 infants with proctocolitis were evaluated retrospectively. All of the infants were diagnosed by a paediatric allergist and/or a paediatric gastroenterologist according to guidelines. Clinical features of the infants, as well as results of a complete blood count, skin prick test, specific immunoglobulin E, and stool examinations or colonoscopy were recorded. Cow's milk (97.7%) was the most common allergen, followed by egg (22%). Forty-five (34.1%) infants had allergies to more than one food. Infants with multiple food allergies had a higher eosinophil count (613±631.2 vs. 375±291.9) and a higher frequency of positive specific IgE and/or positive skin prick test results than that of patients with a single food allergy. Most of the patients whose symptoms persisted after two years of age had multiple food allergies. There is no difference in clinical presentations between infants with single and multiple food allergies. However, infants with multiple food allergies have a high blood total eosinophil count and are more likely to have a positive skin prick test and/or positive specific IgE results. Copyright © 2017 SEICAP. Published by Elsevier España, S.L.U. All rights reserved.

  11. [A case of anaphylaxis due to rose-flavored soft-serve ice cream with pollen food allergy syndrome].

    Science.gov (United States)

    Kitabayashi, Taeru; Sato, Sayuri; Adachi, Mitsuru

    2013-05-01

    We experienced a 10-year-old boy who had anaphylaxis after eating rose-flavored soft-serve ice cream. The patient felt a sense of discomfort in his throat when eating apple, peach, loquat, Japanese pear, and kiwi fruit. Therefore, we measured specific IgE antibodies to allergen components by ImmunoCAP ISAC. Consequently, the patient gave positive results for all PR-10 proteins from birch, alder, hazel, apple, peach, peanut, hazelnut, and soybean, so we diagnosed him with Pollen Food Allergy Syndrome (PFAS) induced by cross reactivity with pollens of birch family and fruits of rose family. When we conducted the skin prick test as is for red rose syrup because of the belief that anaphylaxis was caused by the rose ingredient contained in rose-flavored soft-serve ice cream, the patient gave a strong positive result. However, the results were negative for rose essence and Food Red No. 2 contained. Subsequently, it was found that red rose syrup contained apple juice. Therefore, we conducted the prick-prick test for apple, and the patient was confirmed to be strongly positive to apple. We thus identified apple as the cause of anaphylaxis. Since there is no legal obligation of labeling specific raw materials when directly selling manufactured and processed food products to general consumers, it is possible for general consumers to mistakenly take them in without knowing the containment of allergic substances. It is believed that the labeling method should be improved in the future.

  12. Respiratory allergy to moth: the importance of sensitization to Bombyx mori in children with asthma and rhinitis.

    Science.gov (United States)

    Araujo, Laura M L; Rosário Filho, Nelson A; Riedi, Carlos A

    2014-01-01

    this study aimed to prepare a silkworm moth (Bombyx mori) antigenic extract and to perform skin prick tests with this extract in patients with allergic respiratory diseases; to evaluate serum specific immunoglobulin E (IgE) to Bombyx mori using ImmunoCAP® system and to report the frequency of positivity between the two methods and with clinical data. this was a cross-sectional study with 99 children and adolescents diagnosed with asthma and/or allergic rhinitis, who had skin reactivity to at least one of the six aeroallergens tested. Clinical data were evaluated: skin prick tests with Bombyx mori in-house extract, and total and specific IgE analysis using ImmunoCAP® were performed. the frequency of Bombyx mori specific IgE was found to be 52.5% and 60% using the skin prick test and ImmunoCAP®, respectively. An association between a positive skin test for Bombyx mori and the presence of allergic rhinitis, atopic dermatitis, and urticaria was observed, but the same was not true for asthma or allergic conjunctivitis. There was no relation with the severity of asthma or rhinitis symptoms. a high frequency of sensitization to Bombyx mori was observed in a selected population of patients with respiratory allergic diseases in the city of Curitiba, state of Paraná, Brazil. The extract prepared from the wings of this moth species is effective in demonstrating this sensitivity. Copyright © 2013 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.

  13. Is it possible to make a diagnosis of raw, heated, and baked egg allergy in children using cutoffs? A systematic review.

    Science.gov (United States)

    Calvani, Mauro; Arasi, Stefania; Bianchi, Annamaria; Caimmi, Davide; Cuomo, Barbara; Dondi, Arianna; Indirli, Giovanni Cosimo; La Grutta, Stefania; Panetta, Valentina; Verga, Maria Carmen

    2015-09-01

    The diagnosis of IgE-mediated egg allergy lies both on a compatible clinical history and on the results of skin prick tests (SPTs) and IgEs levels. Both tests have good sensitivity but low specificity. For this reason, oral food challenge (OFC) is the ultimate gold standard for the diagnosis. The aim of this study was to systematically review the literature in order to identify, analyze, and synthesize the predictive value of SPT and specific IgEs both to egg white and to main egg allergens and to review the cutoffs suggested in the literature. A total of 37 articles were included in this systematic review. Studies were grouped according to the degree of cooking of the egg used for OFC, age, and type of allergen used to perform the allergy workup. In children egg allergy seems very likely when SPTs with egg white extract are ≥4 mm or specific IgEs are ≥1.7 kUA /l. In children ≥2 years, OFC could be avoided when SPTs with egg white extract are ≥10 mm or prick by prick with egg white is ≥14 mm or specific IgE is ≥7.3 kUA /l. Likewise, heated egg allergy can be diagnosed if SPTs with egg white extract are >5 and >11 mm in children egg allergy. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  14. Effect of intrathecal non-NMDA EAA receptor antagonist LY293558 in rats: a new class of drugs for spinal anesthesia.

    Science.gov (United States)

    Von Bergen, Nicholas H; Subieta, Alberto; Brennan, Timothy J

    2002-07-01

    Excitatory amino acid receptors are important for both sensory and motor function in the spinal cord. We studied the effects of intrathecal LY293558, a competitive non-N-methyl-D-aspartate excitatory amino acid receptor antagonist, on motor and sensory function in rats to determine whether drugs blocking these receptors could potentially be used as alternative agents to local anesthetics for spinal anesthesia. Rats were tested before and 15-240 min after intrathecal injection of 5 nmol (in 10 microl) LY293558. Sensory function was tested at the hind paw using withdrawal response to pin prick and withdrawal to pinch with sharp forceps. Motor performance (ambulation, placing reflex, and Rotorod time), blood pressure, and heart rate were also evaluated. Some tests were repeated the next day. Responses after LY293558 were compared to injection of 40 microl bupivacaine, 0.75%. Pin-prick responses at the forepaw, chest, abdomen, hind leg, and hind paw were also examined after intrathecal LY293558. Intrathecal LY293558 blocked both sensory and motor responses through 180 min; complete recovery was present the following day. No change in blood pressure or heart rate occurred. The effects of LY293558 were more pronounced and sustained than those of bupivacaine. Segmental blockade of the response to pin prick was present after LY293558. Drugs like LY293558 that block alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA)/kainate receptors may be an alternative to local anesthetics for spinal anesthesia in humans.

  15. Pollen food allergy syndrome in Turkey: Clinical characteristics and evaluation of its association with skin test reactivity to pollens.

    Science.gov (United States)

    Özdemir, Seςil Kepil; Özgüςlü, Selcan

    2017-09-17

    There is limited data regarding pollen food allergy syndrome (PFAS) in Turkey. To investigate the clinical characteristics and possible risk factors of PFAS in Turkey, and to evaluate if there was an association between skin test reactivity to pollens and presence of PFAS. A total of 254 consecutive adult patients with pollen sensitivity were prospectively recruited. Patients were interviewed with a questionnaire including a list of pollen-associated foods. Patients were classified as having PFAS if they reported clear allergic symptoms compatible with PFAS. All participants underwent skin prick tests (SPT) to a panel of common aeroallergens, prick-to-prick tests with culprit fresh foods were performed in patients who gave consent. Self-reported PFAS was observed in 49 patients (19.3%). The most common culprit foods were kiwi, peach, tomato, melon and watermelon. Multiple logistic regression analysis showed that potential risk factors for PFAS were having asthma (OR=2.392, P=0.044) and tree pollen sensitization (OR=2.904, P=0.004). There were no significant differences in the SPT wheal sizes to individual pollen extracts between patients with and without PFAS with a positive SPT result for that pollen extract (P> 0.05). PFAS is frequent in pollen sensitized adults in Turkey. The most commonly implicated foods are kiwi, peach,tomato, melon and watermelon, in our geographical region. SPT wheal sizes to pollen extracts seems to be similar in patients with and without PFAS.

  16. Neuropsychologic status at the age 4 years and atopy in a population-based birth cohort.

    Science.gov (United States)

    Julvez, J; Torrent, M; Guxens, M; Antó, J M; Guerra, S; Sunyer, J

    2009-09-01

    Mental health has been reported to be associated with allergy, but only a few cohort studies have assessed if neurodevelopment predicts atopy. To investigate if neurobehavioral status of healthy 4-year-old children was associated with specific immunoglobulin E (IgE) at the same age and skin prick test results 2 years later. A population-based birth cohort enrolled 482 children, 422 of them (87%) provided neurobehavioral data, 341 (71%) had specific IgE measured at the age of 4 years; and 395 (82%) had skin prick tests completed at the age of 6 years. Atopy was defined as IgE levels higher than 0.35 kU/l to any of the three tested allergens at the age of 4 or as a positive skin prick test to any of the six tested allergens at the age of 6. McCarthy Scales of Child Abilities and California Preschool Social Competence Scale were the psychometric instruments used. Twelve percent of children at the age of 4 and 17% at the age of 6 were atopic. Neurobehavioral scores were negatively associated with 6-year-old atopy after adjustment for socio-demographic and allergic factors, A relative risk of 3.06 (95% CI: 1.30-7.24) was associated with the lowest tertile (scorings eczema at the age of 6, but not at the age of 4, were associated with neurodevelopment at the age of 4. Neuropsychologic functioning and later atopy are negatively associated in preschool age children.

  17. Knowledge and experiences of needle prick injuries (NPI) among ...

    African Journals Online (AJOL)

    of acquiring blood-borne infections from the workplace. ... Adequate knowledge and adherence to safety practices could prevent the occurrence of NPI and the related ..... managers, develop surveillance systems, immunise against hepatitis.

  18. Knowledge and experiences of needle prick injuries (NPI) among ...

    African Journals Online (AJOL)

    Data collected included factors contributing to NPI and high-risk procedures leading to NPI, as perceived by these students. A knowledge assessment of NPI guidelines, policies and protocols and prevalence of NPI among these students was also done. Results: A response rate of 96 (74%) was achieved. The average age ...

  19. Allergic reactions to raw, pasteurized, and homogenized/pasteurized cow milk

    DEFF Research Database (Denmark)

    Høst, A; Samuelsson, E G

    1988-01-01

    Five children aged 12-40 months with IgE-mediated adverse reactions to cow milk (immediate onset clinical pattern of cow milk allergy) were orally challenged double-blind in random order with three different milk preparations processed from the same batch of milk 1) raw untreated cow milk, 2......) pasteurized cow milk, 3) homogenized and pasteurized cow milk, and 4) Nutramigen (a commercial hypoallergenic infant formula based on hydrolysed casein) as placebo. Skin prick tests with the same preparations were also performed. On oral challenge the three different processed milk types provoked significant...... and similar allergic reactions in each child, and no adverse reactions followed the challenge with placebo (Nutramigen). Skin prick test with the same milk products were positive in all children and comparable to the results with an extract of purified raw cow milk protein (Soluprick), whereas Nutramigen did...

  20. Regional somatic embryogenesis from in vitro apple leaf and artificial seed of apple

    International Nuclear Information System (INIS)

    Da Kedong; Li Yazhi; Shu Huairui; Wang Bin

    1998-01-01

    First three open leaves of in vitro apple (Malus domastica Borkh.) shoots were used as explants. Each leaf was divided into three regions (upper, middle and lower) from tip to base and each region was composed of two sub-regions (leaf and right) along the main vain. Every explant was pricked with a dissector at the center of one sub-region before inoculated on MS + BA 1 mg/L + NAA 4 mg/L + 2,4-D 0.5 mg/L medium and incubated in darkness for 7 days, then transferred to MS + BA 1 mg/L medium. 85% of the explants regenerated somatic embryos directly around the prick after 40 days. The embryos were encapsulated in 4% sodium alginate and 2% CaCl 2 as artificial seeds, which could germinate and grow into plantlets under aseptic condition

  1. Thyroid stimulating hormone levels in newborns and early life exposure to endocrine disrupting chemicals - analysis of three European mother-child cohorts

    NARCIS (Netherlands)

    de Cock, Marijke; de Boer, Michiel R; Govarts, Eva; Iszatt, Nina; Palkovicova, Lubica; Lamoree, Marja H; Schoeters, Greet; Eggesbø, Merete; Trnovec, Tomas; Legler, Juliette; van de Bor, Margot

    2017-01-01

    BACKGROUND: Various studies report interactions between thyroid hormones and early life chemical exposure. Our objective was to analyse associations between markers of endocrine disrupting chemical exposure and thyroid function in newborns, determined in heel prick blood spots. METHODS: Three

  2. Occupational Asthma Induced by Chrysonilia sitophila in a Worker Exposed to Coffee Grounds▿

    OpenAIRE

    Francuz, Beata; Yera, Helene; Geraut, Laurent; Bensefa-Colas, Lynda; Nghiem, Zuong Hung; Choudat, Dominique

    2010-01-01

    A new case of occupational asthma caused by Chrysonilia sitophila (asexual state of Neurospora sitophila) was diagnosed by molecular identification of the mold and confirmed by skin prick test, peak expiratory flow rate measurements, and experimental immunoglobulin E analysis.

  3. Occupational asthma induced by Chrysonilia sitophila in a worker exposed to coffee grounds.

    Science.gov (United States)

    Francuz, Beata; Yera, Helene; Geraut, Laurent; Bensefa-Colas, Lynda; Nghiem, Zuong Hung; Choudat, Dominique

    2010-10-01

    A new case of occupational asthma caused by Chrysonilia sitophila (asexual state of Neurospora sitophila) was diagnosed by molecular identification of the mold and confirmed by skin prick test, peak expiratory flow rate measurements, and experimental immunoglobulin E analysis.

  4. Disposal and Reuse of Bergstrom Air Force Base, Texas. Final Environmental Impact Statement.

    Science.gov (United States)

    1993-07-01

    Engelmannia bipinnatifida), wild honeysuckles ( Gaura spp.), mouse-eared chickweeds (Cerastium spp.), stork’s-bills (Erodium spp.), and plantains (Plantago spp...mesquite, other prominent vegetation includes Roosevelt weed (Baccharis neglecta), prickly pear (Opuntia lindheimeri ) and pencil cacti (0. leptocarpis

  5. Napa River Salt Marsh Restoration Project. Volume 1: Environmental Impact Statement

    Science.gov (United States)

    2004-06-01

    Spartina alternifolia), which outcompetes and hybridizes with the native California cord grass. Coon Island, however, immediately adjacent to ponds in...sweet fennel (Foeniculum vulgare), and prickly lettuce . Urban Urban vegetation is dominated by landscaped grasses, flowers, shrubs, and trees, both

  6. Healthcare professionals' perceptions of alcohol- intoxicated trauma ...

    African Journals Online (AJOL)

    2012-08-21

    Aug 21, 2012 ... psychomotor impairment of balance and movement, both of which increase the ... All the doctors and nurses caring for patients in the ED in South Rand ..... the threat of needle-prick injuries, exposure to body fluids, cuts from ...

  7. Causes and Triggers

    Science.gov (United States)

    ... eczema become itchy, or experience a “prickly heat” sensation when they sweat, or get too hot. This can happen when you exercise or wear too many clothes to bed. During the cold winter months, your skin may also get too ...

  8. In vivo and in vitro

    African Journals Online (AJOL)

    contact dermatitis reactions due to other additives in gloves,. e.g. thiurams and ... common inhalant allergens including house-dust mite, cat, dog, grasses, mould ... atopic, as defined by their having a positive skin-prick test for one or more of ...

  9. NATIVE AND INTRODUCED LARVAL FISHES IN SUISAN MARSH, CALIFORNIA,: THE EFFECTS OF FRESHWATER FLOW

    Science.gov (United States)

    We sampled ichthyoplankton weekly in Suisun Marsh in the San Francisco Estuary from February to June each year from 1994 to 1999. We collected approximately 227,900 fish, predominantly shimofuri goby Tridentiger bifasciatus (60%) and prickly sculpin Cottus asper (33%). Principal ...

  10. Occupational Asthma Induced by Chrysonilia sitophila in a Worker Exposed to Coffee Grounds▿

    Science.gov (United States)

    Francuz, Beata; Yera, Helene; Geraut, Laurent; Bensefa-Colas, Lynda; Nghiem, Zuong Hung; Choudat, Dominique

    2010-01-01

    A new case of occupational asthma caused by Chrysonilia sitophila (asexual state of Neurospora sitophila) was diagnosed by molecular identification of the mold and confirmed by skin prick test, peak expiratory flow rate measurements, and experimental immunoglobulin E analysis. PMID:20685936

  11. Food allergy: Two case reports and management challenges in a ...

    African Journals Online (AJOL)

    care physicians in developing countries are still unaware of the condition, especially in ... there was a history of occasional bloody streaks in the stools. There ... namely skin prick tests and IgE levels, were not done owing to financial ...

  12. Formulación de una mermelada a partir de pulpa y cáscara de tunas (Opuntia spp. elaborada a nivel planta piloto Formulación de una mermelada a partir de pulpa y cáscara de tunas (Opuntia spp. elaborada a nivel planta piloto

    Directory of Open Access Journals (Sweden)

    José Luis Magaña Ramírez

    2012-02-01

    Full Text Available After a series of experimental tests of jam pulp-based and peel of prickly pear fruit at a laboratory level, generated from previous research projects, and based on results of sensory evaluation, microbiological and physicochemical analysis, it was selected a formulation for a jam from a mixture of pulp and peel of prickly pear fruit “reyna” variety, and “xoconostle” prickly pear fruit pulp for processing at the pilot platform. The jam produced was characterized by physicochemical analysis and microbiological. In the physicochemical analysis, it was included the determination of dietetic fiber (4.35 g dietetic fiber/15 g jam, being one of the most important evaluation, which value comes mainly from the skin of prickly pear fruit. From the physicochemical analysis, it was designed a label for the jam produced. Based on the specifications required by the “Norma Oficial Mexicana”, the product can be considered suitable for consumption and marketing purposes. Después de una serie de pruebas experimentales de mermeladas a base de pulpa y cás­cara de tunas a nivel laboratorio, generados de proyectos de investigación anteriores, y con base en resultados de evaluación sensorial, análisis microbiológico y fisicoquímico, se seleccionó una formulación de mermelada a partir de una mezcla de pulpa y cáscara de tuna variedad reyna, y pulpa de tuna variedad xoconostle para su procesamiento a nivel planta piloto. La mermelada elaborada fue caracterizada mediante análisis fisicoquímico y microbiológico. Dentro de los análisis fisicoquímicos se incluyó la determinación de Fibra Dietética (4.35 g de fibra dietética/15 g de mermelada, siendo éste uno de los análisis más importantes, cuyo valor procede principalmente de la cáscara de las tunas. Basados en las especificaciones exigidas por la Norma Oficial Mexicana, el producto puede considerarse apto para su consumo y para propósitos de comercialización.

  13. Fulltext PDF

    Indian Academy of Sciences (India)

    System5

    , arranged in two whorls. Fruit is dark-green, prickly, with a sour-sweet white pulp enclosing many reddish brown seeds. The extracts of leaves are known to be cytotoxic and quite effective in treatment of Herpes virus. Used as chemotherapy in ...

  14. Risk estimates of HIV and HBV infection to the dental operator via ...

    African Journals Online (AJOL)

    RISK ESTIMATES OF HIV AND HBV INFECTION. TO THE DENTAL OPERATOR VIA. PRICK ACCIDENTS. W.H. van Palenstein Helderman. Department of Community and Preventive Dentistry,. Faculty of Dentistry, Muhirilbili University College of Health Sciences,. Dar es Salaam, Tanzania. , Introduction. Intact skin provides ...

  15. High-performance liquid chromatography determination of dapsone, monoacetyldapsone, and pyrimethamine in filter paper blood spots

    DEFF Research Database (Denmark)

    Rønn, A M; Lemnge, M M; Angelo, H R

    1995-01-01

    A high-performance liquid chromatography method for the simultaneous analysis of dapsone (DDS), the major metabolite of DDS, monoacetyldapsone (MADDS), and pyrimethamine (PYR) was modified for capillary blood samples obtained by finger prick and dried on filter paper. Limit of quantitation using...

  16. epidemiological hiv surveys

    African Journals Online (AJOL)

    antenatal clinics. Additional ... antenatal clinics is not a satisfactory solution as these are too crude ... trained health care worker [HCW], while a DSS from a heel prick can ... Rankuwa Hospital, a large 1 400-bed referral hospital on the border of ...

  17. Geographical variation in the prevalence of sensitization to common aeroallergens in adults

    DEFF Research Database (Denmark)

    Newson, R B; van Ree, R; Forsberg, B

    2014-01-01

    BACKGROUND: Geographical variation in the prevalence of sensitization to aeroallergens may reflect differences in exposure to risk factors such as having older siblings, being raised on a farm or other unidentified exposures. OBJECTIVE: We wanted to measure geographical variation in skin prick te...

  18. To the biology of the Damalacantha vacca (Fischer von Waldheim, 1846 (Orthoptera: Tettigonnidae: Bradyporinae with the description of eggs

    Directory of Open Access Journals (Sweden)

    I. I. Temreshev

    2017-12-01

    Full Text Available The article gives a new data on the biology and fecundity of the grasshopper Damalacantha vacca (Fischer von Waldheim, 1846, a rare species of the Orthopterans, listed in the Red Book of the Republic of Kazakhstan. Earlier in the literature it was noted that this species eats saltwort (Salsola. When the Damalacantha vacca was kept in the cage, in addition to plant foods (except for Amaranthaceae and Chenopodiaceae plants, cereals, mostly spikelets and young leaves, and thinly sliced pieces of various vegetables - cabbage, potatoes, carrots were eaten, and animals (dead grasshoppers, flies and butterflies - Owlet moths. Egg laying by females carried out in batches of 5-6 pieces per soil, with preference given to areas with compacted soil, permeated with interlaced plant roots. Fecundity of two females, who made a full clutch, was 49 and 54 eggs, respectively. For the first time the description of the egg is given and the structure of the chorion.

  19. Phytoextraction and phytostabilization potential of plants grown in the vicinity of heavy metal-contaminated soils: a case study at an industrial town site.

    Science.gov (United States)

    Lorestani, B; Yousefi, N; Cheraghi, M; Farmany, A

    2013-12-01

    With the development of urbanization and industrialization, soils have become increasingly polluted by heavy metals. Phytoremediation, an emerging cost-effective, nonintrusive, and aesthetically pleasing technology that uses the remarkable ability of plants to concentrate elements, can be potentially used to remediate metal-contaminated sites. In this research, two processes of phytoremediation (phytoextraction and phytostabilization) were surveyed in some plant species around an industrial town in the Hamedan Province in the central-western part of Iran. To this purpose, shoots and roots of the seven plant species and the associated soil samples were collected and analyzed by measuring Pb, Fe, Mn, Cu, and Zn concentrations using ICP-AES and then calculating the biological absorption coefficient, bioconcentration factor, and translocation factor parameters for each element. The obtained results showed that among the collected plants, Salsola soda is the most effective species for phytoextraction and phytostabilization and Cirsium arvense has the potential for phytostabilization of the measured heavy metals.

  20. Analysis of oilseed of Halophytic species

    Directory of Open Access Journals (Sweden)

    Parto Roshandel

    2015-07-01

    Full Text Available Seeds of Atriplex griffithii, Haloxylon ammodendron, Salicornia europaea and Salsola yazdiana were analyzed to determine their potential as sources of edible oil. The quantity of total oil varied from 13.8% in Atriplex griffithii to 20.9% in H. ammodendron. The proportion of unsaturated fatty acids were higher (62-73.8%, with the highest values of α-linoleic acid (18.6%, linoleic acid (28.6% and oleic acid (19.7% in the seeds of A. griffithii, H. ammodendron and S. europaea, respectively. Results of physicochemical evaluation of the extracted oils ranged as follows: iodine values, 99.8-106.5 (g I2/100 g; saponification value, 188-283 (mg KOH/1g of oil; peroxide value, 9-13 (meq./kg and refractive index, 1.4750- 1.4761. Amongst these oilseeds, S. europaea (containing 73.8% unsaturated fatty acids but not erucic acid was the highest in quality for human consumption followed by H. ammodendron.

  1. Differential phytosociological interactions involving male and female atriplex bonnevillensis

    Science.gov (United States)

    Sinclair, J.; Emlen, J.M.; Rinella, M.; Snelgrove, J.; Freeman, D.C.

    2009-01-01

    Wind-pollinated dioecious plants often exhibit spatial segregation of the sexes. This partial niche separation has most often been explored using abiotic niche axes. However, if the sexes are truly separated in space, then they are apt to encounter different plant species that may heavily affect growth and reproduction. Also, to the extent that their niches differ, the sexes may respond differently to the same co-occurring species. Here we examine interspecific interactions that influence male and female reproductive potential in Atriplex bonnevillensis. Using Emlen's interaction assessment, a technique which assesses species interactions based on cover classes, we show that Salsola species compete significantly with females but not males, while Halogeton glomeratus competes with males but not females. The effect of competition only became apparent when we corrected for site-specific fertility. These results imply that differential competition must be considered when studying dioecious plants that display spatial segregation of the sexes.

  2. Oral nickel exposure may induce Type I hypersensitivity reaction in nickel-sensitized subjects.

    Science.gov (United States)

    Büyüköztürk, Suna; Gelincik, Aslı; Ünal, Derya; Demirtürk, Mustafa; Çelik, Dolay Damla; Erden, Sacide; Çolakoğlu, Bahattin; Erdem Kuruca, Serap

    2015-05-01

    Little is known about the clinical and immunological changes in the nickel allergic patients with systemic symptoms. We aimed to evaluate T helper cell responses of patients with different clinical presentations due to nickel. Patients having various allergic symptoms and positive patch test results to nickel and 20 controls underwent skin prick tests with nickel. IL-10, IL-4, IL-5 and IFN-gamma were measured in the culture supernatants of PBMC stimulated by nickel during lymphocyte proliferation test (LTT). 69 patients (56 female, mean age: 49.2 ± 13.1), 97% having nickel containing dental devices and 20 controls (8 female, mean age 34.9 ± 12.06) were evaluated. Skin prick tests with nickel were positive in 70% of the patients (pnickel. Nickel containing dental alloys and oral nickel intake seem to trigger systemic symptoms in previously nickel sensitized patients. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Soy sauce allergy.

    Science.gov (United States)

    Sugiura, K; Sugiura, M

    2010-07-01

    Soy sauce is well-known as a Japanese traditional seasoning, namely shoyu. Usually, shoyu means sauce made from soy. Shoyu does have not only benefits but also adverse effects. Soy sauce allergy which is not caused by soy or wheat allergy is rare. Our four patients developed cellulites and dermatitis around lips with irritation after a meal with shoyu. The age of the patients was 10, 35, 46 and 51 years; they were all female. These inflammations can be developed by two causes; first it can be caused by allergic reactions to shoyu; the second, it can be caused by histamine poisoning. It is important to determine whether inflammation is caused by allergic reactions or histamine poisoning. We determined the volume of histamine in some sauces and performed prick test and laboratory tests. Four patients had positive reactions by prick test after using some sauces. We suspected that histamine caused their symptoms, but nine normal volunteers had negative reactions. Patient's specific IgE score to soy and wheat was class 0. The results showed that the sauce made from soybean and broad bean contained histamine, but histamine in other sauces was not detected. In this study, we confirmed by prick test, four cases of soy sauce allergy, which was caused by some products during brewing. When patients with inflammations around mouth, after a meal containing or using soy sauce, are examined, it should be considered whether dermatitis or cellulites were developed by allergic reaction or by histamine poisoning.

  4. Pruritic and Nociceptive Sensations and Dysesthesias From a Spicule of Cowhage

    Science.gov (United States)

    LaMotte, R. H.; Shimada, S. G.; Green, B. G.; Zelterman, D.

    2009-01-01

    Although the trichomes (spicules) of a pod of cowhage (Mucuna pruriens) are known to evoke a histamine-independent itch that is mediated by a cysteine protease, little is known of the itch and accompanying nociceptive sensations evoked by a single spicule and the enhanced itch and pain that can occur in the surrounding skin. The tip of a single spicule applied to the forearm of 45 subjects typically evoked 1) itch accompanied by nociceptive sensations (NS) of pricking/stinging and, to a lesser extent, burning, and 2) one or more areas of cutaneous dysesthesia characterized by hyperknesis (enhanced itch to pricking) with or without alloknesis (itch to stroking) and/or hyperalgesia (enhanced pricking pain). Itch could occur in the absence of NS or one or more dysesthesias but very rarely the reverse. The peak magnitude of sensation was positively correlated for itch and NS and increased (exhibited spatial summation) as the number of spicules was increased within a spatial extent of 6 cm but not 1 cm. The areas of dysesthesia did not exhibit spatial summation. We conclude that itch evoked by a punctate chemical stimulus can co-exist with NS and cutaneous dysesthesias as may occur in clinical pruritus. However, cowhage itch was not always accompanied by NS or dysesthesia nor was a momentary change in itch necessarily accompanied by a similar change in NS or vice versa. Thus there may be separate neural coding mechanisms for itch, nociceptive sensations, and each type of dysesthesia. PMID:19144738

  5. Occupational protein contact dermatitis.

    Science.gov (United States)

    Barbaud, Annick; Poreaux, Claire; Penven, Emmanuelle; Waton, Julie

    2015-01-01

    Occupational contact dermatitis is generally caused by haptens but can also be induced by proteins causing mainly immunological contact urticaria (ICU); chronic hand eczema in the context of protein contact dermatitis (PCD). In a monocentric retrospective study, from our database, only 31 (0.41%) of patients with contact dermatitis had positive skin tests with proteins: 22 had occupational PCD, 3 had non-occupational PCD, 5 occupational ICU and 1 cook had a neutrophilic fixed food eruption (NFFE) due to fish. From these results and analysis of literature, the characteristics of PCD can be summarized as follows. It is a chronic eczematous dermatitis, possibly exacerbated by work, suggestive if associated with inflammatory perionyxix and immediate erythema with pruritis, to be investigated when the patient resumes work after a period of interruption. Prick tests with the suspected protein-containing material are essential, as patch tests have negative results. In case of multisensitisation revealed by prick tests, it is advisable to analyse IgE against recombinant allergens. A history of atopy, found in 56 to 68% of the patients, has to be checked for. Most of the cases are observed among food-handlers but PCD can also be due to non-edible plants, latex, hydrolysed proteins or animal proteins. Occupational exposure to proteins can thus lead to the development of ICU. Reflecting hypersensitivity to very low concentrations of allergens, investigating ICU therefore requires caution and prick tests should be performed with a diluted form of the causative protein-containing product. Causes are food, especially fruit peel, non-edible plants, cosmetic products, latex, animals.

  6. Implementation of POCT in the diabetic clinic in a large hospital

    African Journals Online (AJOL)

    public health concern worldwide especially in develop- ing countries.4 T2DM ... the correlation between the use of venous and capillary. (finger prick) blood for HbA1c .... not going to be significantly affected by the haemoglo- bin variants in our ...

  7. Two episodes of anaphylaxis following exposure to hydroxypropyl methylcellulose during cataract surgery

    DEFF Research Database (Denmark)

    Munk, Sofie J; Heegaard, Steffen; Mosbech, Holger

    2013-01-01

    UNLABELLED: We report a case of immediate severe anaphylaxis to hydroxypropyl methylcellulose (HPMC) on 2 separate occasions during cataract surgery in a 71-year-old patient. Skin prick tests were positive for HPMC, a constituent of Ocucoat and Xylocaine gel, which were administered intraocularly...

  8. Occupational and food allergy: focus on allergen extracts

    NARCIS (Netherlands)

    N.W. de Jong (Nicolette)

    2004-01-01

    textabstractThe aim of this thesis is, first, the diagnostic work-up of occupational and food allergies in the absence of well-validated commercially available standardised extracts for Skin Prick Test. Second, to investigate cross-reactivity in occupational and food allergic patients. Third, the

  9. Antifreeze proteins enable plants to survive in freezing conditions

    Indian Academy of Sciences (India)

    1. Acaenamagellanica. Prickly burr. Doucet et al. 2000. 2. Acer saccharoides. Maple. Doucet et al. 2000. 3. Agrostistenuis. Creeping bentgrass. Doucet et al. 2000. 4. Alliarapetiolata. Garlic mustard. Urrutia et al. 1992. 5. Ammopiptanthusmongolicus. Evergreen legume. Wang et al. 2003. 6. Aster cordifolius. Wood aster.

  10. Management of First Branchial Cleft Anomalies via a Cartilage-Splitting Technique.

    Science.gov (United States)

    Schmidt, Richard; Conrad, David; Field, Erin; O'Reilly, Robert

    2015-06-01

    First branchial cleft anomalies are uncommon lesions that often present as periauricular infections. They have high recurrence rates, due in part to scarring secondary to prior infections and their management. These lesions have a close relationship with the facial nerve, and most authors recommend its identification and dissection because of this relationship. Nonetheless, facial nerve palsy has been reported in up to 15% of cases. We describe a novel technique for the management of first branchial cleft anomalies. Such lesions that presented in an infra- or postauricular location were approached via an incision through the cartilage of the pinna, between the tragus and antitragus. This technique affords direct access to the lesion without the need for facial nerve dissection. Six patients were treated. Five had prior surgery, including 3 with previous attempts at excision. There were no complications. The median follow-up was 35 months. One patient developed a recurrence. © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2015.

  11. Comparison of the Efficacy of Dry Needling and High-Power Pain Threshold Ultrasound Therapy with Clinical Status and Sonoelastography in Myofascial Pain Syndrome.

    Science.gov (United States)

    Aridici, Rifat; Yetisgin, Alparslan; Boyaci, Ahmet; Tutoglu, Ahmet; Bozdogan, Erol; Sen Dokumaci, Dilek; Kilicaslan, Nihat; Boyaci, Nurefsan

    2016-10-01

    The aim of this study was to compare the therapeutic efficacy of high-power pain threshold (HPPT) ultrasound therapy applied to the trigger points and dry needling (DN) in myofascial pain syndrome. Sixty-one patients were randomly assigned to an HPPT (n = 30) and dry needling (n = 31) groups. The primary outcome measures were the Visual Analog Scale (VAS) and Neck Pain and Disability Scale (NPDS), both at 1 week and 4 weeks after treatment. The secondary outcome measures were the number of painful trigger points, range of the tragus-acromioclavicular joint, the Short Form-36, the Beck Depression Inventory, the Beck Anxiety Inventory, and sonoelastographic tests after a 1-week treatment. More improvement was seen in anxiety in the HPPT group (P 0.05). A decrease in tissue stiffness was only seen in the HPPT group (P pain syndrome. Although a significant decrease was shown in tissue stiffness with HPPT, neither of these treatments had an apparent superiority.

  12. Measurement of hemodynamics during postural changes using a new wearable cephalic laser blood flowmeter.

    Science.gov (United States)

    Fujikawa, Tetsuya; Tochikubo, Osamu; Kura, Naoki; Kiyokura, Takanori; Shimada, Junichi; Umemura, Satoshi

    2009-10-01

    Patients with orthostatic hypotension have pathologic hemodynamics related to changes in body posture. A new cephalic laser blood flowmeter that can be worn on the tragus to investigate the hemodynamics upon rising from a sitting or squatting posture was developed. The relationship between cephalic hemodynamics and cerebral ischemic symptoms in 63 subjects in a sitting, squatting, and standing positions using the new device was evaluated. Transient decrease in blood pressure within 15 s after rising to an erect position possibly causes dizziness, syncope, and fall. Subjects exhibiting dizziness upon standing showed a significant decrease in the cephalic blood flow (CBF) and indirect beat-to-beat systolic blood pressure, as monitored by the Finometer, and a significant correlation was observed between the drop ratio (drop value on rising/mean value in the squatting position) of CBF and that of systolic blood pressure. This new wearable CBF-meter is potentially useful for estimating cephalic hemodynamics and objectively diagnosing cerebral ischemic symptoms of subjects in a standing posture.

  13. Up-date on neuro-immune mechanisms involved in allergic and non-allergic rhinitis

    NARCIS (Netherlands)

    van Gerven, L.; Boeckxstaens, G.; Hellings, P.

    2012-01-01

    Non-allergic rhinitis (NAR) is a common disorder, which can be defined as chronic nasal inflammation, independent of systemic IgE-mediated mechanisms. Symptoms of NAR patients mimic those of allergic rhinitis (AR) patients. However, AR patients can easily be diagnosed with skin prick test or

  14. Emerging crops in the USDA arid lands germplasm collection

    Science.gov (United States)

    The USDA National Plant Germplasm System maintains collections of several emerging crops for arid lands at the National Arid Land Plant Genetic Resources Unit in Parlier, CA (NALPGRU). The guayule, jojoba, and prickly pear collections are most active in terms of current research and crop development...

  15. MACROSCOPICAL AND MICROSCOPICAL STUDIES ON THE ...

    African Journals Online (AJOL)

    Caesalpinia crista leaves are bipinnate of about six pairs with alternate leaflets while the stem us fibrous, cylindrical hollow and prickly. Microscopical examination revealed the presence of strained cuticle, straight-walled epidermal cells, paracytic stomata, unicellular covering trichomes, fibres, prisms as well as cluster of ...

  16. Exophiala asiatica, a new species from a fatal case in China

    NARCIS (Netherlands)

    Li, D.M.; Li, R.Y.; de Hoog, G.S.; Wang, Y.X.; Wang, D.L.

    2009-01-01

    We describe a new species, Exophiala asiatica, isolated from an infection of the pharynx in a 20-year-old, immunocompetent woman in Nanjing, China. The infection was initiated by a fishbone prick in the pharynx, soon developed with facial nodules but subsequently seemed to have disappeared. Tonsil

  17. Sensitization pattern of crustacean-allergic individuals can indicate allergy to molluscs

    DEFF Research Database (Denmark)

    Vidal, C; Bartolomé, B; Rodríguez, V

    2015-01-01

    This study investigated the sensitization pattern of crustacean-allergic patients according to tolerance to molluscs. Thirty-one patients with anaphylaxis to crustaceans (14 with mollusc allergy and 17 with mollusc tolerance) were studied using skin prick tests (SPTs), specific IgEs (sIgEs) and SDS-PAGE...

  18. The prevalence of plant food allergies: A systematic review

    DEFF Research Database (Denmark)

    Zuidmeer, Laurian; Goldhahn, Klaus; Rona, Roberto J.

    2008-01-01

    ) in the literature database MEDLINE focused on fruits, vegetables/legumes, tree nuts, wheat, soy, cereals, and seeds. Prevalence estimates were categorized by food item and method used (food challenges, skin prick test, serum IgE, parent/self-reported symptoms), complemented by appropriate meta-analyses. Results: We...

  19. Poor biologic activity of cross-reactive IgE directed to carbohydrate determinants of glycoproteins

    NARCIS (Netherlands)

    van der Veen, M. J.; van Ree, R.; Aalberse, R. C.; Akkerdaas, J.; Koppelman, S. J.; Jansen, H. M.; van der Zee, J. S.

    1997-01-01

    BACKGROUND: In our outpatient population, approximately one third of patients sensitized to grass pollen were found to have significant serum levels of anti-peanut IgE in the RAST, without positive peanut skin prick test (SPT) response and without peanut-related allergic symptoms. It was suggested

  20. Archaeological Investigations of Three Sites within the Wipp Core Area, Eddy County, New Mexico.

    Science.gov (United States)

    1985-06-01

    leaves are eaten as food (Castetter 1935:33). Cactaceae c macroMeris Texas devilshead Echinocereaus pi s Caespitose hedgehog E. fendleri Fendler hedgehog...Ountia ad.isii Davis cholla . kleiniae Klein cholla 0. ept is Christmas cactus 0. phaeacantha Prickly pear Fruits of most cactaceae are edible. The

  1. Screening of antiproliferative effect of aqueous extracts of plant foods consumed in México on the breast cancer cell line MCF-7.

    Science.gov (United States)

    García-Solís, Pablo; Yahia, Elhadi M; Morales-Tlalpan, Verónica; Díaz-Muñoz, Mauricio

    2009-01-01

    We evaluated the antiproliferative effect of aqueous extracts of 14 plant foods consumed in Mexico on the breast cancer cell line MCF-7. The plant foods used were avocado, black sapote, guava, mango, prickly pear cactus stems (called nopal in Mexico, cooked and raw), papaya, pineapple, four different cultivars of prickly pear fruit, grapes and tomato. β-Carotene, total phenolics and gallic acid contents and the antioxidant capacity, measured by the ferric reducing/antioxidant power and the 2,2-diphenyl-1,1-picrylhydrazyl radical scavenging assays, were analyzed in each aqueous extract. Only the papaya extract had a significant antiproliferative effect measured with the methylthiazolydiphenyl-tetrazolium bromide assay. We did not notice a relationship between the total phenolic content and the antioxidant capacity with antiproliferative effect. It is suggested that each extract of plant food has a unique combination of the quantity and quality of phytochemicals that could determine its biological activity. Besides, papaya represents a very interesting fruit to explore its antineoplastic activities.

  2. [Accidental exposure to blood by midwives in French maternity units: results of the national surveillance 2003].

    Science.gov (United States)

    Vincent, A; Cohen, M; Bernet, C; Parneix, P; L'Hériteau, F; Branger, B; Talon, D; Hommel, C; Abiteboul, D; Coignard, B

    2006-05-01

    Midwives appear to be the health care workers exposed to the highest rates of bloodborne injury. In this paper - based on a national survey - we describe the bloodborne injuries occurring in this profession. During the year 2003, 241 hospitals took part in a national survey of bloodborne injuries. Employees registered anonymous standardized reports of bloodborne events with the Occupational Medicine Unit. The data were processed by the coordination center for the fight against nosocomial infections (C. CLIN) which is in charge of the national analysis of all the events reported in this database. 169 of the 6973 bloodborne events reported during 2003 (2.4%), were signed by midwives or midwife students. The first three most frequent accidents reported were: ocular projections during childbirth, pricks when repairing episiotomy, pricks or cuts when handling soiled instruments. Improving knowledge of risk as well as promotion of protection/prevention measures well adapted to this profession should be helpful in optimizing future attitudes.

  3. Intercommunications of indexes of speed and power qualities of sportsmen single combat on the stage of the specialized base preparation

    Directory of Open Access Journals (Sweden)

    A.O. Nykytenko

    2013-01-01

    Full Text Available The purpose of work is a study of interdependence between indexes specially-preparatory and general preparatory exercises of sportsmen (boxing, sporting hand-to-hand fight hopak, fencing. 42 sportsmen took part in researches. Statistical reliable intercommunications are set: a indexes of force of shots by hands with the indexes of rate of single movement; b indexes of force of shots by feet with indexes from maximal force of muscles (tractive forces; c indexes of speed of shots by hands (pricking with the indexes of distance of shove of balls by mass 300 gramme; d indexes of speed of movement a step in a battle bar with indexes: speeds of shots by hands (pricking; e indexes of frequency of shots of boxers and sportsmen hand-to-hand fight hopak with the indexes of frequency of movement (manoeuvring on feet. Facilities which it is expedient to apply in the training process of single combats on this stage of long-term preparation are certain.

  4. Atopic dermatitis of the face, scalp, and neck

    DEFF Research Database (Denmark)

    Jensen-Jarolim, E; Poulsen, L K; With, H

    1992-01-01

    We have previously reported that a lipophilic yeast, Pityrosporum ovale (P. ovale) produced a high frequency of positive skin prick tests and in vitro histamine-release (HR) tests in patients suffering from atopic dermatitis (AD) of the face, scalp, and neck. In the present study, our aim was to ...

  5. Prevalence of Occupational Accidents/Injuries among Health Care ...

    African Journals Online (AJOL)

    BACKGROUND: Health care workers (HCWs) are prone to occupational accidents and injuries such as needle pricks in the course of their day to day activities in the health care setting. OBJECTIVE: To determine the prevalence of needle sticks and other occupational exposures among HCWs in a Nigerian tertiary hospital.

  6. 78 FR 32246 - Pesticide Products; Registration Applications for New Active Ingredients

    Science.gov (United States)

    2013-05-29

    ...; pitaya, fruit; prickly pear cactus, pads; cattle/goat/hog/horse/ sheep, fat; cattle/goat/hog/horse/sheep, meat; cattle/goat/hog/horse/ sheep, meat byproducts; milk; poultry, eggs; poultry, meat; and poultry, meat byproducts. Contact: Jessica Rogala, (RD), (703) 347- 0263, email address: [email protected] epa.gov...

  7. Capacities of Candidate Herbaceous Plants for Phytoremediation of Soil-based TNT and RDX on Ranges

    Science.gov (United States)

    2008-09-01

    installations and distribution of Sida spinosa...Lehi, UT • Forb seeds from three vendors as follows: redroot pigweed (Amaran- thus retroflexus), morning glory (Ipomoea lacunosa), and prickly sida ... Sida spinosa) from Azlin Seed Service, Leland, MS; common milk- weed (Asclepias syriaca) from Prairiemoon Nursery, Winona, WI; common purslane

  8. GALEN skin test study III: Minimum battery of test inhalent allergens needed in epidemiological studies in patients

    NARCIS (Netherlands)

    Bousquet, P.-J.; Burbach, G.; Heinzerling, L. M.; Edenharter, G.; Bachert, C.; Bindslev-Jensen, C.; Bonini, S.; Bousquet-Rouanet, L.; Demoly, P.; Bresciani, M.; Bruno, A.; Gjomarkaj, M.; Canonica, G. W.; Darsow, U.; Durham, S.; Fokkens, W. J.; Giavi, S.; Gramiccioni, C.; Papadopoulos, N. G.; Haahtela, T.; Kowalski, M. L.; Magyar, P.; Muraközi, G.; Orosz, M.; Röhnelt, C.; Stingl, G.; Todo-Bom, A.; von Mutius, E.; Wiesner, A.; Wöhrl, S.; Bousquet, J.; Zuberbier, T.

    2009-01-01

    Background: The number of allergens to be tested in order to identify sensitized patients is important in order to have the most cost-effective approach in epidemiological studies. Objective: To define the minimal number and the type of skin prick test (SPT) allergens required to identify a patient

  9. Unpredictability of regression of analgesia during the continuous postoperative extradural infusion of bupivacaine

    DEFF Research Database (Denmark)

    Mogensen, T; Hjortsø, N C; Bigler, D

    1988-01-01

    Twenty-four otherwise healthy patients scheduled for elective major abdominal surgery received general anaesthesia plus lumbar extradural analgesia. A loading dose of 0.5% plain bupivacaine was given to produce sensory analgesia (pin prick) from T4 to S5 and followed by a continuous infusion of 0...

  10. Are basophil histamine release and high affinity IgE receptor expression involved in asymptomatic skin sensitization?

    DEFF Research Database (Denmark)

    Jensen, Bettina Margrethe; Assing, K; Jensen, Lone Hummelshøj

    2006-01-01

    Immunoglobulin (Ig)E-sensitized persons with positive skin prick test, but no allergy symptoms, are classified as being asymptomatic skin sensitized (AS). The allergic type 1 disease is dependant on IgE binding to the high affinity IgE-receptor (FcepsilonRI) expressed on basophils and mast cells....

  11. 76 FR 81393 - Extension of Tolerances for Emergency Exemptions (Multiple Chemicals)

    Science.gov (United States)

    2011-12-28

    ... limited to: Crop production (NAICS code 111). Animal production (NAICS code 112). Food manufacturing... control of mayweed chamomile and prickly lettuce in Washington and Idaho. This regulation extends a time... seed at 0.02 ppm for an additional 3-year period. These tolerances will expire and are revoked on...

  12. Determination of heavy metals in chinese prickly ash from different ...

    African Journals Online (AJOL)

    Methods: CPA samples collected from different production areas in China were subjected to microwave digestion, and the contents of copper (Cu), nickel (Ni), chromium (Cr), lead (Pb), cadmium (Cd), arsenic (As), mercury (Hg), tin (Sn), and antimony (Sb) were determined by inductively coupled plasma-mass spectrometry ...

  13. Diagnosis of mold allergy by RAST and skin prick testing.

    Science.gov (United States)

    Nordvall, S L; Agrell, B; Malling, H J; Dreborg, S

    1990-11-01

    Sera from 33 patients with mold allergy proven by bronchial provocation were analyzed for specific IgE against six mold species comparing an improved Phadebas RAST with four other techniques. The new method was more sensitive and gave significantly higher IgE antibody concentrations for all tested molds except Cladosporium herbarum.

  14. Needle prick injury at work: The experiences of professional nurses ...

    African Journals Online (AJOL)

    African Journal for Physical Activity and Health Sciences. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 18 (2012) >. Log in or Register to get access to full text downloads.

  15. Determination of heavy metals in chinese prickly ash from different ...

    African Journals Online (AJOL)

    digestion, and the contents of copper (Cu), nickel (Ni), chromium (Cr), lead (Pb), cadmium ... concentrations of heavy metals in these CPA samples mean they are safe for human consumption. ... poisoning, including Pb, Cd, As, Hg, Sn, and Sb.

  16. Similar prevalence, different spectrum: IgE-mediated food allergy among Turkish adolescents.

    Science.gov (United States)

    Mustafayev, R; Civelek, E; Orhan, F; Yüksel, H; Boz, A B; Sekerel, B E

    2013-01-01

    Scarcity of reliable data on food allergy prevalence exists in Turkey. We aimed to assess reported and confirmed IgE-mediated food allergy prevalence, and define the spectrum of allergenic food. We prospectively evaluated the ISAAC Phase II study population for food allergy. Participants that reported experiencing food allergy symptom in the last year and/or were skin prick test positive for a predefined list of food allergens, were interviewed via telephone, and those considered as having food allergy were invited to undergo clinical investigation, including challenge tests. A total of 6963 questionnaires were available. Parental reported food allergy prevalence and skin prick sensitisation rate were 20.2 ± 0.9% and 5.9 ± 0.6%. According to the above-defined criteria, 1162 children (symptom positive n=909, skin prick test positive n=301, both positive n=48) were selected and 813 (70.0%) were interviewed via telephone. Out of 152 adolescents reporting a current complaint, 87 accepted clinical investigation. There were 12 food allergies diagnosed in nine adolescents, with food allergy prevalence of 0.16 ± 0.11%. The most common foods involved in allergic reactions were walnut (n=3) and beef meat (n=2), followed by hen's egg (n=1), peanut (n=1), spinach (n=1), kiwi (n=1), cheese (n=1), hazelnut (n=1) and peach (n=1). While parental reported food allergy prevalence was within the range reported previously, confirmed IgE-mediated food allergy prevalence among adolescents was at least 0.16%, and the spectrum of foods involved in allergy differed from Western countries, implying environmental factors may play a role. Copyright © 2012 SEICAP. Published by Elsevier Espana. All rights reserved.

  17. Opisthorchis felineus negatively associates with skin test reactivity in Russia-EuroPrevall-International Cooperation study.

    Science.gov (United States)

    Fedorova, O S; Janse, J J; Ogorodova, L M; Fedotova, M M; Achterberg, R A; Verweij, J J; Fernández-Rivas, M; Versteeg, S A; Potts, J; Minelli, C; van Ree, R; Burney, P; Yazdanbakhsh, M

    2017-07-01

    Most studies on the relationship between helminth infections and atopic disorders have been conducted in (sub)tropical developing countries where exposure to multiple parasites and lifestyle can confound the relationship. We aimed to study the relationship between infection with the fish-borne helminth Opishorchis felineus and specific IgE, skin prick testing, and atopic symptoms in Western Siberia, with lifestyle and hygiene standards of a developed country. Schoolchildren aged 7-11 years were sampled from one urban and two rural regions. Skin prick tests (SPT) and specific IgE (sIgE) against food and aeroallergens were measured, and data on allergic symptoms and on demographic and socioeconomic factors were collected by questionnaire. Diagnosis of opisthorchiasis was based on PCR performed on stool samples. Of the 732 children included, 34.9% had opisthorchiasis. The sensitization to any allergen when estimated by positive SPT was 12.8%, while much higher, 24.0%, when measured by sIgE. Atopic symptoms in the past year (flexural eczema and/or rhinoconjunctivitis) were reported in 12.4% of the children. SPT was positively related to flexural eczema and rhinoconjunctivitis, but not to wheezing. Opisthorchiasis showed association with lower SPT response, as well as borderline association with low IgE reactivity to any allergen. However, the effect of opisthorchiasis on SPT response was not mediated by IgE, suggesting that opisthorchiasis influences SPT response through another mechanism. Opisthorchiasis also showed borderline association with lower atopic symptoms. There is a negative association between a chronic helminth infection and skin prick test reactivity even in a developed country. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. Lepidoglyphus destructor acarus in the urban house environment.

    Science.gov (United States)

    Juliá, J C; Martorell, A; Ventas, P; Cerdá, J C; Torró, I; Carreira, J; Guinot, E; Sanz, J; Alvarez, V

    1995-01-01

    To assess the presence of Lepidoglyphus destructor in the household environment of sensitized children living in an urban environment, samples of house dust were collected at the homes where two groups of patients were living, as well as in two bakeries in the city of Valencia, which were taken as a reference. Patients were divided into two groups. Group A included atopic children suffering from rhinitis and/or asthma, who were sensitized to L. destructor, as proven by prick test and specific IgE (CAP). Group B included children with the same features as those included in Group A, who were sensitized to Dermatophagoides pteronyssinus, with prick and CAP tests showing no significant sensitization to L. destructor. The samples of dust were analyzed, and the amounts of Der p I, Der f I, Der II and Lep d I per gram of dust were assessed through a solid-phase ELISA with monoclonal antibodies. In Group A, all patients but two showed a sensitization to D. pteronyssinus by prick test and serum IgE. At the homes of the patients from both groups, significant levels of Dermatophagoides were found. In Group A, only three houses showed levels of L. destructor which were comparable to those found in bakeries. Lep d I was not found in the houses of Group B patients. This means that a sensitization to L. destructor, as assessed with full extracts, is not always an indicator of its presence at the patient's house environment; it may rather refer to cross-reactivity to Dermatophagoides. Thus, availability of the main antigen Lep d I seems necessary to increase the specificity of the allergologic study.

  19. IgE, IgG4 and IgA specific to Bet v 1-related food allergens do not predict oral allergy syndrome.

    Science.gov (United States)

    Guhsl, E E; Hofstetter, G; Lengger, N; Hemmer, W; Ebner, C; Fröschl, R; Bublin, M; Lupinek, C; Breiteneder, H; Radauer, C

    2015-01-01

    Birch pollen-associated plant food allergy is caused by Bet v 1-specific IgE, but presence of cross-reactive IgE to related allergens does not predict food allergy. The role of other immunoglobulin isotypes in the birch pollen-plant food syndrome has not been investigated in detail. Bet v 1-sensitized birch pollen-allergic patients (n = 35) were diagnosed for food allergy by standardized interviews, skin prick tests, prick-to-prick tests and ImmunoCAP. Concentrations of allergen-specific IgE, IgG1, IgG4 and IgA to seven Bet v 1-related food allergens were determined by ELISA. Bet v 1, Cor a 1, Mal d 1 and Pru p 1 bound IgE from all and IgG4 and IgA from the majority of sera. Immunoglobulins to Gly m 4, Vig r 1 and Api g 1.01 were detected in allergy and increased or reduced levels of IgE, IgG1, IgG4 or IgA specific to most Bet v 1-related allergens. Api g 1-specific IgE was significantly (P = 0.01) elevated in celeriac-allergic compared with celeriac-tolerant patients. Likewise, frequencies of IgE (71% vs 15%; P = 0.01) and IgA (86% vs 38%; P = 0.04) binding to Api g 1.01 were increased. Measurements of allergen-specific immunoglobulins are not suitable for diagnosing Bet v 1-mediated plant food allergy to hazelnut and Rosaceae fruits. In contrast, IgE and IgA to the distantly related allergen Api g 1 correlate with allergy to celeriac. © 2014 The Authors. Allergy Published by John Wiley & Sons Ltd.

  20. Screening of lettuce germplasm for agronomic traits under low water conditions

    Science.gov (United States)

    After a preliminary screening of over 3,500 varieties, we selected 200 cultivars of butterhead, cos, crisphead, leaf, and stem lettuce (Lactuca sativa L.) and wild prickly lettuce (Lactuca serriola L.) to test under high water (150% ET) and low water (50% ET) conditions in the field, and tracked com...

  1. Elevated levels of IgG and IgG4 to Malassezia allergens in atopic eczema patients with IgE reactivity to Malassezia

    NARCIS (Netherlands)

    Johansson, Catharina; Tengvall Linder, Maria; Aalberse, Rob C.; Scheynius, Annika

    2004-01-01

    The opportunistic yeast Malassezia is considered to be one of the factors that can contribute to atopic eczema (AE). Elevated serum IgE levels, T-cell proliferation and positive skin prick test (SPT) and atopy patch test (APT) reactions to Malassezia are found among AE patients. Sera from 127 AE

  2. サンショウのすりこ木に発生したタイワンメダカカミキリ(甲虫)

    OpenAIRE

    岩田, 隆太郎; Ryutaro, IWATA; 京都大学木材研究所:(現)日本大学農獣医学部林学科

    1991-01-01

    In an apartment building in Yokohama, Japan, a brood of Stenhomalus taiwanus MATSUSHITA (Coleoptera: Cerambycidae: Cerambycmae) was observed to infest a kitchen pestle made of a stem of Japanese prickly ash, Zanthoxylum piperitum (L.). Adult emergence was observed indoors firstly in late summer to early autumn, and then in next spring.

  3. CHAPTER 1

    African Journals Online (AJOL)

    Dr Olaleye

    history of IPTp-Sulfadoxine-Pyrimethamine (SP) use in the index pregnancy were recorded. Finger and heel pricks blood samples from mothers and neonates respectively were used to detect malaria parasitaemia and to determine packed cell volume (PCV). Median number of ANC visits made by the enrollees was 4.0 ...

  4. Long-term mortality among adults with asthma

    DEFF Research Database (Denmark)

    Ali, Zarqa; Dirks, Christina Glattre; Ulrik, Charlotte Suppli

    2013-01-01

    from an out-patient clinic in 1974 to 1990, and followed up until the end of 2011. Subjects were classified as having allergic or non-allergic asthma on the basis of detailed history, spirometric tests, tests for IgE-mediated allergy (skin prick tests and RAST), and bronchial challenge tests...

  5. Creating a Successful Academic Climate for Urban Students

    Science.gov (United States)

    Slaughter, Terri

    2009-01-01

    Teaching students in the inner city has been likened to hugging a porcupine--teachers nudge them toward success while getting pricked along the way. Many urban students perform below proficiency level and are difficult to manage. Their apathy toward completing class assignments, let alone homework, compounds the problem. As a whole, educators do…

  6. Coming up………

    African Journals Online (AJOL)

    2006-11-24

    Nov 24, 2006 ... prick skin of potato liberally: intra-microwave-oven mayhem otherwise. Similar havoc has led to abandonment of instant- poached-egg-in-a-mug formula: a mere two seconds is the difference between perfection and disaster. Since then one has progressed to pasta, having mastered the art of microwaving ...

  7. Download this PDF file

    African Journals Online (AJOL)

    with respiratory allergies such as rhinitis and asthma, high levels of serum IgE, specific IgE, and positive skin-prick tests to aeroallergens or foods. Immunological differences between NAAEDS and AAEDS can be found in the cell and cytokine patterns in peripheral blood and. The aetiopathogenesis of atopic dermatitis (AD) ...

  8. Relationship of distraction rate with inferior alveolar nerve degeneration-regeneration shift

    Directory of Open Access Journals (Sweden)

    Ying-hua Zhao

    2018-01-01

    Full Text Available Distraction osteogenesis is an important technique for the treatment of maxillofacial abnormities and defects. However, distraction osteogenesis may cause the injury of the inferior alveolar nerve. The relationship between distraction rate and nerve degeneration-regeneration shift remains poorly understood. In this study, 24 rabbits were randomly divided into four groups. To establish the rabbit mandibular distraction osteogenesis model, the mandibles of rabbits in distraction osteogenesis groups were subjected to continuous osteogenesis distraction at a rate of 1.0, 1.5 and 2.0 mm/d, respectively, by controlling rounds of screwing each day in the distractors. In the sham group, mandible osteotomy was performed without distraction. Pin-prick test with a 10 g blunt pin on the labium, histological and histomorphometric analyses with methylene blue staining, Bodian's silver staining, transmission electron microscopy and myelinated fiber density of inferior alveolar nerve cross-sections were performed to assess inferior alveolar nerve conditions. At 28 days after model establishment, in the pin-prick test, the inferior alveolar nerve showed no response in the labium to a pin pricks in the 2 mm/d group, indicating a severe dysfunction. Histological and histomorphometric analyses indicated that the inferior alveolar nerve suffered more degeneration and injuries at a high distraction rate (2 mm/d. Importantly, the nerve regeneration, indicated by newborn Schwann cells and axons, was more abundant in 1.0 and 1.5 mm/d groups than in 2.0 mm/d group. We concluded that the distraction rate was strongly associated with the inferior alveolar nerve function, and the distraction rates of 1.0 and 1.5 mm/d had regenerative effects on the inferior alveolar nerve. This study provides an experimental basis for the relationship between distraction rate and nerve degeneration-regeneration shift during distraction osteogenesis, and may facilitate reducing nerve

  9. IN TIME VARIATION OF PATHERGY PHENOMENON IN BEHCET'S DISEASE

    Directory of Open Access Journals (Sweden)

    C. Chams F. Davatchi

    1996-07-01

    Full Text Available The pathergy phenomenon (PP is one of the hallmarks of Behcet's Disease (BD. It has been reported to be positive from 17.5% to 83% of cases in different studies. PP is used as a diagnosis criterion in the Dilsen criteria, the Japan criteria, the International criteria, and the Iran criteria. It is therefore important to know wether PP is a fix manifestation of the disease or a cyclic manifestation like mucocutaneous disorders. To evaluate the charaterist'tc of P'P over a period of time, 77 patients were selected randomly (rcgaardless of their disease activity or their disease manifestations, to have a second pathergy test, lite pathcrgy test was performed by needle puncture on the skin of the forearm. Three methods were used: 1- needle prick with a 21 - gauge needle. 2- needle prick with a 25  gauge needle. 3- needle prick with a 25-guage needle and the injection of I drop of a sterile normal saline solution. Tlie result was evaluated 24 hours Liter. Tlie shortest interval between two tests-was one month and the longest 13 years. Die mean interval was 35.4 months, the standard deviation was 36.6 Tlurty-nine patients had a negative PI' at their first evaluation. At the second evaluation, 27 remained negative while 12 changed to positive. Thirty-eight patients had a positive PP at their first evaluation. At the second evaluation, 19 remained positive while 19 changed to negative. Although there is a difference between the two groups, it is not statistically significant (x=3.813 , p=0.05. Our data demonstrates that the pathergy phenomenon is waxing and waning, like the majority of other manifestations of Behcet's Disease. TJierefore, it may be repeated for diagnosis purposes.

  10. The diagnostic value of component-resolved diagnostics in peanut allergy in children attending a Regional Paediatric Allergology Clinic.

    Science.gov (United States)

    van Veen, Leonieke N; Heron, Michiel; Batstra, Manou; van Haard, Paul M M; de Groot, Hans

    2016-06-02

    To date, diagnosing food allergies in children still presents a diagnostic dilemma, leading to uncertainty concerning the definite diagnosis of peanut allergy, as well as to the need for strict diets and the potential need for adrenalin auto-injectors. This uncertainty in particular is thought to contribute to a lower quality of life. In the diagnostic process double-blind food challenges are considered the gold standard, but they are time-consuming as well as potentially hazardous. Other diagnostic tests have been extensively studied and among these component-resolved diagnostics appeared to present a promising alternative: Ara h2, a peanut storage protein in previous studies showed to have a significant predictive value. Sixty-two out of 72 children, with suspected peanut allergy were analyzed using serum specific IgE and/or skin prick tests and specific IgE to several components of peanut (Ara h 1, 2, 3, 6, 8, 9). Subsequently, double-blind food challenges were performed. The correlation between the various diagnostic tests and the overall outcome of the double-blind food challenges were studied, in particular the severity of the reaction and the eliciting dose. The double-blind provocation with peanut was positive in 33 children (53 %). There was no relationship between the eliciting dose and the severity of the reaction. A statistically significant relationship was found between the skin prick test, specific IgE directed to peanut, Ara h 1, Ara h 2 or Ara h 6, and the outcome of the food challenge test, in terms of positive or negative (P food challenge. This study shows that component-resolved diagnostics is not superior to specific IgE to peanut extract or to skin prick testing. At present, it cannot replace double-blind placebo-controlled food challenges for determination of the eliciting dose or the severity of the peanut allergy in our patient group.

  11. [Analysis of Therapeutic Regularities and Characteristics of Blood-letting Therapy for Acne Patients Based on Data Mining].

    Science.gov (United States)

    Du, Yu-zhu; Jia, Chun-sheng; Wang, Jian-ling; Shi, Jing; Zhang, Xiao-xu; Liu, Xin; Gang, Wei-juan

    2015-06-01

    To analyze the therapeutic regularities and characteristics of blood-letting therapy for acne in the past clinical practice by using data mining. Original papers about acne treated by pricking blood therapy were searched and screened from common databases as Chinese National Knowledge Infrastructure Database (CNKI), WanFang Data, SinoMed, Ovid, ScienceDirect, Socolar, SciFinder, Foreign Medical Journal Full-Text Service (FMJS) and PubMed using keywords of acne+bleeding therapy, acne+blood-letting, acne+ pricking blood, followed by establishing a data plateform to conduct a data mining using Online Analytical Processing (OLAP). A total of 230 original journal articles about acne treated by pricking blood therapy were collected. The included acne cases with wind-heat pattern were predoment, being 56 in frequency-times and acounting for 24. 78 %. In the treatment of acne, the therapeutic tool, three-edged needle was often used, being 168 in frequency and acounting for 71.79%. The frequently employed acupoints were those of the Governor Vessel and Bladder Meridian, such as Dazhui (GV 14) and back-shu points. When auricular points used for blood-letting, Erjian (EX-HN 6) and the Vena of the auricular back were most frequently selected. In addition to blood-letting, other therapies such as Chinese herbal medicines, filiform needles, and otopoint-pellet pressure were also used in combination, being 166 in items and constituting 72. 17%. Generally, blood-letting treatment was conducted once every three days (twice a week) or once every two days (three times a week) for about 20 sessions for each acne patient. Blood-letting therapy is effective in the treatment of acne. But if used in combination with other therapies, the therapeutic effect would be better.

  12. Dreng med diarré og svær dehydrering havde food protein-induced enterocolitis syndrome

    DEFF Research Database (Denmark)

    Henriksen, Pernille; Børresen, Malene Landbo; Dahl, Kathrin

    2016-01-01

    Food protein-induced enterocolitis syndrome (FPIES) is a rare non-IgE mediated condition. Symptoms of acute FPIES include vomiting, diarrhoea and dehydration. Symptoms are often misread as acute abdomen or sepsis. The condition can be fatal. There are no biomarkers for FPIES, and skin prick test...... confronted with the very ill, dehydrated infant....

  13. Experience on impregnation of wood.en poles with water borne salts

    African Journals Online (AJOL)

    I. Leechability. Since the process depends on osmosis, the preservative must be highly soluble in water and rains may wash it away. 2. Diminished Mechanical Strength. Pricking of the pole at and around the ground line will diminish the mechanical strength of the pole. 3. Cost. Most important of all, the cost of the preserva-.

  14. Change Agent Survival Guide

    Science.gov (United States)

    Dunbar, Folwell L.

    2011-01-01

    Consulting is a rough racket. Only a tarantula hair above IRS agents, meter maids and used car sales people, the profession is a prickly burr for slings and arrows. Throw in education, focus on dysfunctional schools and call oneself a "change agent," and this bad rap all but disappears. Unfortunately, though, consulting/coaching/mentoring in…

  15. Population ecology of small mammals on the radioactive waste management complex, Idaho National Engineering Laboratory

    International Nuclear Information System (INIS)

    Groves, C.R.; Keller, B.L.

    1983-01-01

    Species composition, diversity, biomass, population dynamics, absolute density, and movements of small mammal populations were examined on and adjacent to a solid radioactive waste disposal area in southeastern Idaho. The 15-month live-trapping study resulted in marking 2384 individuals representing 10 species of small mammals. Three vegetation types were sampled: crested wheatgrass (Agropyron cristatum) and Russian thistle (Salsola kali) habitats on the disposal area and native sagebrush (Artemisia tridentata) habitat surrounding the disposal area. The deer mouse (Peromyscus maniculatus) was the most common rodent in both disposal area habitats as well as the adjacent sagebrush habitat; Ord's kangaroo rat (Dipodomys ordii) was also an abundant rodent in all vegetation types. The montane vole (Microtus montanus) was common only in crested wheatgrass stands on the disposal area. The annual total small mammal biomass of 346 kg for the entire disposal area represents a potentially large vector for movement of radionuclides off the disposal area. However, the number of animals known to contact waste areas and traverse at least 50 m beyond the perimeter of the SDA appears to be small (8.7%)

  16. Impact of highly saline wetland ecosystem on floral diversity of the Cholistan desert

    International Nuclear Information System (INIS)

    Gill, A.H.; Ahmad, K.S.; Habib, S.; Ahmad, S.A.; Nawaz, T.; Ahmad, F.

    2012-01-01

    The impact of highly saline wetland ecosystem created under Salinity Control and Reclamation Project (SCARP) on floral diversity was investigated in the arid environments of Cholistan Desert. Species richness, diversity indices and evenness indices were worked out to look at the distance at which the salt water has altered the native vegetation. Four sites including SCARP ponds of different ages (S1, S2, S3 and S4), and a reference site (SR) were selected for vegetation studies and data were recorded by 1 x 1 m quadrats, which were laid on permanent transect lines. Salt water showed great influence on ecological parameters of the native vegetation up to 40 m. Multivariate (cluster) analysis showed close clustering of highly salt tolerant species, Aeluropus lagopoides, Tamarix dioica and Suaeda fruticosa in one group, and relatively less tolerant Crotalaria burhia, Cyperus conglomeratus, Indigofera argentea, Haloxylon salicornicum, Haloxylon stocksii, Neurada procumbens and Salsola baryosma in second group. Moderately salt tolerant Aristida adscensionis, Lasiurus scindicus and Sporobolus iocladus were clustered in a separate group. (author)

  17. Amoxicillin rash in patients with infectious mononucleosis: evidence of true drug sensitization.

    Science.gov (United States)

    Ónodi-Nagy, Katinka; Kinyó, Ágnes; Meszes, Angéla; Garaczi, Edina; Kemény, Lajos; Bata-Csörgő, Zsuzsanna

    2015-01-01

    It hasn't been clearly understood yet whether sensitization to antibiotics, the virus itself or transient loss of drug tolerance due to the virus, is responsible for the development of maculopapular exanthems following amoxicillin intake in patients with infectious mononucleosis. We aimed to examine whether sensitization to penicillin developed among patients with skin rash following amoxicillin treatment within infectious mononucleosis. Ten patients were investigated for drug sensitization by lymphocyte transformation test and six patients were further tested by prick-, intradermal and patch tests employing the penicillin's main antigens. Lymphocyte transformation test showed negative results with amoxicillin, while one patient had positive reaction to cefixime. Six patients with suspected sensitization to amoxicillin were then investigated by in vivo tests. Prick tests were negative in all six patients, but the intradermal tests showed positive reactions in four patients. Our data demonstrate that in vitro testing is not sensitive enough in determining drug sensitization to penicillin. In vivo tests should be performed to detect sensitization and indeed with skin tests our results confirmed that sensitization to aminopenicillin may develop within infectious mononucleosis.

  18. Clinical correlations of dry eye syndrome and allergic conjunctivitis in Korean children.

    Science.gov (United States)

    Kim, Tae Hyung; Moon, Nam Ju

    2013-01-01

    Clinical patterns in pediatric patients with dry eye syndrome and allergic conjunctivitis were investigated. Children aged 6 to 15 years with dry eye symptoms were included. Slit-lamp examinations including tear film break-up time, Schirmer's test, and fluorescent staining were performed, and subjective symptoms were investigated. Patients with allergic conjunctivitis were subjected to skin prick tests. Tear film break-up time was shorter and the number of symptoms related to dry eyes was higher in pediatric patients with allergic conjunctivitis than in those without allergic conjunctivitis. Patients with allergic conjunctivitis who had higher numbers of positive allergens on the skin prick test also had shorter tear film break-up time. Because pediatric patients with dry eye syndrome tend to complain less about their symptoms than adult patients, dry eye syndrome is commonly overlooked. This study showed that dry eyes tended to be more severe with the presence of allergic conjunctivitis; the more allergens present, the more severe the dry eyes. More attention should be paid to the treatment of pediatric patients with dry eyes accompanied by allergies. Copyright 2013, SLACK Incorporated.

  19. [Penicillin allergy as a diagnostic problem. Overview and personal studies].

    Science.gov (United States)

    Walker, T; Jung, E G; Bayerl, C

    2000-11-01

    Penicillin allergy is a common clinical problem. The distinction between penicillin and para-infectious exanthems is difficult. We investigated the reliability of the history, as well as the sensitivity and specificity of skin tests and specific IgE levels. 160 patients with a history of penicillin allergy were retrospectively evaluated in the outpatient department of a dermatological clinic. Nearly 50% were diagnosed as allergic to penicillin by detection of specific IgE or skin test. About 60% of the patients with immediate type reactions, and 72% with maculo-papular erythema showed positive reactions in skin tests. Significantly more patients were diagnosed as allergic to penicillin by intradermal testing than by prick testing (p sensitivity of the specific IgE RAST was 17.9%; the specifity, 89.5%. For the prick test the sensitivity was 8.2%; the specificity 90.8%. For the intradermal test the sensitivity was 26%; the specifity 69.7%. We suggest a step by step procedure to detect penicillin allergy making the diagnostic results as valid as possible.

  20. Activation of eggs of the frog, Rana nigromaculata Hallowell, after localized UV-irradiation

    International Nuclear Information System (INIS)

    Sambuichi, Hajime

    1981-01-01

    To analyze the mode of transmission of the activating stimulus, the change in time course of the 2nd maturation division was examined in eggs of the frog, Rana nigromaculata, which were activated by insemination or by pricking after UV-irradiation at either the animal or the vegetal hemisphere. Irradiation of the animal (UAF) or the vegetal (UVF) hemisphere before insemination retarded the 2nd maturation division in comparison with that in normally fertilized eggs (CF). When the eggs were pricked at the irradiated surface (UAP and UVP, respectively), however, the 2nd maturation division progressed faster than that of CF. The comparison of the velocity of the 2nd maturation division between UAF and UVF, and also between UAP and UVP, showed that the cortical reaction in the vegetal hemisphere was affected by UV-irradiation more severely than that in the animal hemisphere. The results suggested that the quality of the granules or the constituent within the egg cortex might vary according to the regional difference in the egg surface. (author)

  1. Damage caused by Tetranychus Merganser Bondreaux (Acari: Tetranychidae on Nopal Verdura Opuntia Ficus-Indica (L. Miller during winter

    Directory of Open Access Journals (Sweden)

    J. Lima-Espíndola

    2017-09-01

    Full Text Available The spider mite Tetranychus merganser occurs in some crops in Mexico and USA. This mite could be considered a potential pest in countries like Japan. In this work we recorded the damages that T. merganser causes to the nopal verdura (prickly pear vegetable crop in one of the principal cactus crop regions of Mexico.

  2. clinical features of malaria parasiteamia among children in parts of ...

    African Journals Online (AJOL)

    LUCY

    This study was carried out to investigate the prevalence of malaria parasitamiae and its clinical features in children aged 0-5 years in parts of Delta State of Nigeria. Blood samples were randomly collected from the thumb of each child using the finger prick method. A total of 600 blood samples (360 males and 240 females) ...

  3. Cat sensitization according to cat window of exposure in adult asthmatics

    NARCIS (Netherlands)

    Oryszczyn, M.-P.; van Ree, R.; Maccario, J.; Nadif, R.; Kauffmann, F.

    2009-01-01

    P>Background In adults, there is limited information on tolerance to cat, which may be reflected by high IgG(4) without IgE sensitization. Early exposure to cat may play a critical role. Objective The aim was to assess among adults the association of Fel d 1 IgG(4), Fel d 1 IgE, skin prick test

  4. Abelmoschus manihot var. pungens (Roxburgh Hochreutiner (Malvaceae, A Newly Naturalized Plant in Taiwan

    Directory of Open Access Journals (Sweden)

    Szu-I Hsieh

    2013-06-01

    Full Text Available Abelmoschus manihot var. pungens (Roxburgh Hochreutiner is naturalized in abandoned land of central Taiwan. The distinguished characteristics of A. manihot var. pungens include the ovate-lanceolate epicalyx lobes, distinct prickly hairs all over the plant, and even over the margins of its epicalyx lobes. Descriptions, line drawings and photos of this species are provided.

  5. Role of offending out-door aero-allergen and CD14 C(-159)T ...

    African Journals Online (AJOL)

    Child- hood-onset and adult-onset of asthma showed significant difference in allergen sensitivity as well as genetic background with respect to CD14 polymorphism. Keywords: Asthma, aero-allergen, skin prick test, total IgE, CD14 gene polymorphism. DOI: https://dx.doi.org/10.4314/ahs.v17i4.18. Cite as: Dutta S, Mondal P, ...

  6. Fulltext PDF

    Indian Academy of Sciences (India)

    Is the above explanation really correct? Does the experiment show air has mass? For, if we consider the buoyancy force of the ambient air acting on each balloon then the weight of the air inside is exactly cancelled by the buoyancy force. Then both before and after pricking the balloons should be in balance: the net force ...

  7. Cultured Mast Cells from Patients with Asthma and Controls Respond with Similar Sensitivity to Recombinant Der P2-Induced, IgE-Mediated Activation

    DEFF Research Database (Denmark)

    Krohn, I K; Sverrild, A; Lund, G

    2013-01-01

    for mite allergen Der p2. The sensitivity of IgE-mediated activation of mast cells was investigated as FcεRI-mediated upregulation of CD63. Ten subjects were atopic, defined as a positive skin prick test (>3 mm) to at least one of ten common allergens. After activation with recombinant Der p2, the maximum...

  8. Molecular based assessment of genetic diversity of xoconostle ...

    African Journals Online (AJOL)

    aghomotsegin

    2014-01-08

    Jan 8, 2014 ... Xoconostle or acidic prickly pear is an important fruit in Mexico; it is produced by a ... study, we report for the first time the estimation of genetic diversity within a set ... demonstrates the high genetic variation among genotypes of .... O. leucotricha Salm-Dyck × O. joconostle F.A.C Weber Zacatecas Wild Stock.

  9. The short-term effects of TENS plus therapeutic ultrasound combinations in chronic neck pain.

    Science.gov (United States)

    Sayilir, Selcuk

    2018-05-01

    To investigate the effects of TENS plus therapeutic ultrasound combinations on symptom relief, physical functionality, perceived stress levels, daytime sleepiness and neck mobility in patients with chronic neck pain (CNP). A total of 64 patients were divided into two groups as the TENS plus ultrasound group (n = 39) and the control CNP group (n = 25). The therapy comprised TENS and therapeutic ultrasound applications for 10 sessions. The control subjects were discouraged from using analgesics but were allowed to use paracetamol daily, if necessary. The Neck Disability Index (NDI), Epworth Sleepiness Scale (ESS), Perceived Stress Scale (PSS), visual analog scale (VAS) and tragus-wall/chin-manubrium distances were recorded at the baseline and after therapy. Significant improvements were detected in the TENS plus ultrasound group compared to the control CNP subjects in respect of VAS, PSS and NDI scores after the TENS plus therapeutic ultrasound therapies (all p stress levels and improving functionality in the short-term of CNP. Copyright © 2018 Elsevier Ltd. All rights reserved.

  10. The Effect of Microwave Radiation on Prickly Paddy Melon (Cucumis myriocarpus

    Directory of Open Access Journals (Sweden)

    Graham Brodie

    2012-01-01

    Full Text Available The growing list of herbicide-resistant biotypes and environmental concerns about chemical use has prompted interest in alternative methods of managing weeds. This study explored the effect of microwave energy on paddy melon (Cucumis myriocarpus plants, fruits, and seeds. Microwave treatment killed paddy melon plants and seeds. Stem rupture due to internal steam explosions often occurred after the first few seconds of microwave treatment when a small aperture antenna was used to apply the microwave energy. The half lethal microwave energy dose for plants was 145 J/cm2; however, a dose of at least 422 J/cm2 was needed to kill seeds. This study demonstrated that a strategic burst of intense microwave energy, focused onto the stem of the plant is as effective as applying microwave energy to the whole plant, but uses much less energy.

  11. Kicking against the pricks: vaccine sceptics have a different social orientation.

    Science.gov (United States)

    Luyten, Jeroen; Desmet, Pieter; Dorgali, Veronica; Hens, Niel; Beutels, Philippe

    2014-04-01

    In any country, part of the population is sceptical about the utility of vaccination. To develop successful vaccination programmes, it is important to study and understand the defining characteristics of vaccine sceptics. Research till now mainly focused either on the underlying motives of vaccine refusal, or on socio-demographic differences between vaccine sceptics and non-sceptics. It remained till now unexplored whether both groups differ in terms of basic psychological dispositions. We held a population survey in a representative sample of the population in Flanders, Belgium (N = 1050), in which we investigated whether respondents' attitude to vaccination was associated with their basic disposition toward other community members or society in general, as measured by the Triandis and Gelfand social orientation scale. We found that sceptics and non-sceptics have a different social orientation, even when several variables are controlled for. More specifically, vaccine sceptics scored significantly lower on both horizontal individualism and horizontal collectivism, indicating a lower disposition to see others as equals. These findings need confirmation in the context of different countries. Such insights can be valuable to optimize the design of effective communication strategies on vaccination programmes.

  12. Association between alcohol consumption and skin prick test reactivity to aeroallergens

    DEFF Research Database (Denmark)

    Assing, Kristian; Bødtger, Uffe; Linneberg, Allan

    2007-01-01

    respiratory disease and lifestyle habits, including alcohol consumption. SPT positivity was defined as a positive reaction (> or =3 mm) against at least 1 of 10 common inhalant allergens. RESULTS: Before and after adjustment for sex, age, smoking, atopic predisposition, and pet keeping, no significant...

  13. The Colletotrichum gloeosporioides species complex.

    Science.gov (United States)

    Weir, B S; Johnston, P R; Damm, U

    2012-09-15

    The limit of the Colletotrichum gloeosporioides species complex is defined genetically, based on a strongly supported clade within the Colletotrichum ITS gene tree. All taxa accepted within this clade are morphologically more or less typical of the broadly defined C. gloeosporioides, as it has been applied in the literature for the past 50 years. We accept 22 species plus one subspecies within the C. gloeosporioides complex. These include C. asianum, C. cordylinicola, C. fructicola, C. gloeosporioides, C. horii, C. kahawae subsp. kahawae, C. musae, C. nupharicola, C. psidii, C. siamense, C. theobromicola, C. tropicale, and C. xanthorrhoeae, along with the taxa described here as new, C. aenigma, C. aeschynomenes, C. alatae, C. alienum, C. aotearoa, C. clidemiae, C. kahawae subsp. ciggaro, C. salsolae, and C. ti, plus the nom. nov. C. queenslandicum (for C. gloeosporioides var. minus). All of the taxa are defined genetically on the basis of multi-gene phylogenies. Brief morphological descriptions are provided for species where no modern description is available. Many of the species are unable to be reliably distinguished using ITS, the official barcoding gene for fungi. Particularly problematic are a set of species genetically close to C. musae and another set of species genetically close to C. kahawae, referred to here as the Musae clade and the Kahawae clade, respectively. Each clade contains several species that are phylogenetically well supported in multi-gene analyses, but within the clades branch lengths are short because of the small number of phylogenetically informative characters, and in a few cases individual gene trees are incongruent. Some single genes or combinations of genes, such as glyceraldehyde-3-phosphate dehydrogenase and glutamine synthetase, can be used to reliably distinguish most taxa and will need to be developed as secondary barcodes for species level identification, which is important because many of these fungi are of biosecurity

  14. To the Issue of Search For New Radioprotectors Made of Vegetative Raw Materials of the Former Semipalatinsk Test Site

    International Nuclear Information System (INIS)

    Muzychkina, R.A.; Abilov, Zh.A.; Omarkamzinova, V.B.; Burasheva, G.Sh.; Zhusupova, G.E.; Seisebaev, A.T.; Kadyrova, N.Zh.; Karabalin, B.K.

    1998-01-01

    performed earlier by Moscow State Univ. on study of the radioprotective properties of Salsola colina, Kochia prostrata, Stipa baikalensis, Salsola arbuscula, and other plants eaten by rodents confirm the prospectiveness of the search for effective radioprotectors among the natural compounds of vegetative origin as well as the investigations of Russian Institute for Biophysics on radioprotective effect of preparations extracted from the roots of 'Archancelica officinalis' and top parts of 'Lediumpolustre'. This paper presents analytical results of the chemical composition of some plant species growing on contaminated territories of Balapan Test Field, considers a program of collection of plant specific radioresistant species and obtaining the group of chemical substances made of them on the base of polyphenols, polysaccharides and products of their modification, and describes the methodology of screening of radioprotective activity of compounds using laboratory animals

  15. Lessons from Cacti: How to Survive the Prickles of Life during Tough Times

    Science.gov (United States)

    Bigger, Alan S.; Bigger, Linda B.

    2009-01-01

    The saguaro cactus looked a little like humans, in different shapes and sizes. How on earth do they survive in a climate that seems so inhospitable? It is possible to learn lessons for life from a cactus, if one can only get beyond the thorns, and that these lessons will assist one to survive during tough or prickly times. These plants survive…

  16. Effects of intrathecal bupivacaine and bupivacaine plus sufentanil in ...

    African Journals Online (AJOL)

    2013-03-23

    Mar 23, 2013 ... sensation to cold alcohol swap and pin‑prick test with a 22G hypodermic needle and on each side of the mid‑thoracic line, was measured every 2 min, until it reached the peak level with four consecutive tests and then every 10 min during the surgery. We recorded time for sensory blockade to reach the T10 ...

  17. during the rut, at Ishasha, Queen Elizabeth Park

    African Journals Online (AJOL)

    29° 40'E (Fig. 1). A little rain usually falls in every month but there are two wet seasons in April .... Older adults: With age the rate of horn growth diminishes and the ridges remain only slightly ..... A herdmasler buU. inspecting his harem of females and o:hibiling the 'head-high' posture, ears pricked ... harem bulls lost control.

  18. Archaeological Investigations at Four Sites in the Abiquiu Multiple Resource Area New Mexico,

    Science.gov (United States)

    1983-12-01

    Malvaceae Mallow family _TOpnti&Prickly pear cactus Cvijidr~ni Cholla cactus olmniaceae Phlox family Polygonaceae Knotweed family Ranunculaceae ...of social organization and possibly information on group or family size. 5. Cultural affiliation. The cultural affiliation of those occupations...uppermost sample within the struc- ture, but is probably related to the presence of disturbance plants. Sunflowers and other members of the composite family

  19. Approach to evaluation and management of a patient with multiple food allergies.

    Science.gov (United States)

    Bird, J Andrew

    2016-01-01

    Diagnosing food allergy is often challenging, and validated testing modalities are mostly limited to immunoglobulin E (IgE)-mediated reactions to foods. Use of food-specific IgE tests and skin prick tests in individuals without a history that supports an IgE-mediated reaction to the specific food being tested diminishes the predictive capabilities of the test. To review the literature regarding evaluation of patients with a concern for multiple food allergies and to demonstrate an evidence-based approach to diagnosis and management. A literature search was performed and articles identified as relevant based on the search terms "food allergy," "food allergy diagnosis," "skin prick test," "serum IgE test," "oral food challenge", and "food allergy management." Patients at risk of food allergy are often misdiagnosed and appropriate evaluation of patients with concern for food allergy includes taking a thorough diet history and reaction history, performing specific tests intentionally and when indicated, and conducting an oral food challenge in a safe environment by an experienced provider when test results are inconclusive. An evidence-based approach to diagnosing and managing a patient at risk of having a life-threatening food allergy is reviewed.

  20. Traceability of Opuntia ficus-indica L. Miller by ICP-MS multi-element profile and chemometric approach.

    Science.gov (United States)

    Mottese, Antonio Francesco; Naccari, Clara; Vadalà, Rossella; Bua, Giuseppe Daniel; Bartolomeo, Giovanni; Rando, Rossana; Cicero, Nicola; Dugo, Giacomo

    2018-01-01

    Opuntia ficus-indica L. Miller fruits, particularly 'Ficodindia dell'Etna' of Biancavilla (POD), 'Fico d'india tradizionale di Roccapalumba' with protected brand and samples from an experimental field in Pezzolo (Sicily) were analyzed by inductively coupled plasma mass spectrometry in order to determine the multi-element profile. A multivariate chemometric approach, specifically principal component analysis (PCA), was applied to individuate how mineral elements may represent a marker of geographic origin, which would be useful for traceability. PCA has allowed us to verify that the geographical origin of prickly pear fruits is significantly influenced by trace element content, and the results found in Biancavilla PDO samples were linked to the geological composition of this volcanic areas. It was observed that two principal components accounted for 72.03% of the total variance in the data and, in more detail, PC1 explains 45.51% and PC2 26.52%, respectively. This study demonstrated that PCA is an integrated tool for the traceability of food products and, at the same time, a useful method of authentication of typical local fruits such as prickly pear. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  1. Vitamin A supplementation and risk of atopy: long-term follow-up of a randomized trial of vitamin A supplementation at six and nine months of age.

    Science.gov (United States)

    Kiraly, Nicholas; Balde, Aliu; Lisse, Ida Marie; Eriksen, Helle Brander; Aaby, Peter; Benn, Christine Stabell

    2013-11-19

    The World Health Organization recommends high-dose vitamin A supplementation (VAS) for children above six months of age in low-income countries. VAS has been associated with up-regulation of the Th2 response. We aimed to determine if VAS is associated with atopy in childhood. Infants in Guinea-Bissau were randomly allocated VAS or placebo, either at six and nine months of age, or only at nine months of age. At six months of age, children were furthermore randomized to measles vaccine or inactivated polio vaccine. At nine months of age all children received measles vaccine. Children were revisited seven years later and skin prick testing was performed. Atopy was defined as a skin prick reaction ≥ 3 mm. 40 of 263 children (15%) were atopic. Overall VAS had no significant effect on the risk of atopy (Prevalence Ratio 1.23; 95% CI 0.69-2.18). The Prevalence Ratio was 1.60 (0.66-3.90) for males and 1.00 (0.46-2.15) for females. There was no significant effect of VAS in infancy on atopy later in childhood. The role of infant VAS in the development of atopy is still unclear.

  2. Survey of fishes and environmental conditions in Abbotts Lagoon, Point Reyes National Seashore, California

    Science.gov (United States)

    Saiki, M.K.; Martin, B.A.

    2001-01-01

    This study was conducted to gain a better understanding of fishery resources in Abbotts Lagoon, Point Reyes National Seashore. During February/March, May, August, and November 1999, fish were sampled with floating variable-mesh gill nets and small minnow traps from as many as 14 sites in the lagoon. Water temperature, dissolved oxygen, pH, total ammonia(NH3 + NH4+), salinity, turbidity, water depth, and bottom substrate composition were also measured at each site. A total of 2,656 fish represented by eight species was captured during the study. Gill nets captured Sacramento perch, Archoplites interruptus; largemouth bass, Micropterus salmoides; Pacific herring, Clupea pallasi; prickly sculpin, Cottus asper, silver surfperch, Hyperprosopon ellipticum; longfin smelt, Spirinchus thaleichthys; and striped bass, Morone saxatilis; whereas minnow traps captured Sacramento perch; prickly sculpin; and threespine stickleback, Gasterosteus aculeatus. Cluster analysis (Ward's minimum variance method of fish catch statistics identified two major species assemblages-the first dominated by Sacramento perch and, to a lesser extent, by largemouth bass, and the second dominated by Pacific herring and threespine stickleback. Simple discriminant analysis of environmental variables indicated that salinity contributed the most towards separating the two assemblages.

  3. Neurosensory changes of palatal mucousa following Le Fort I osteotomy

    Directory of Open Access Journals (Sweden)

    Bijan Movahedian Attar

    2009-09-01

    Full Text Available

    • BACKGROUND: This study evaluated the sensation of palatal ucosa before and after Le Fort I osteotomy and compared it based on whether greater palatine nerve has been dissected or not.
    • METHODS: Sixteen patients were studied within one week before  urgery and then one week, 6 weeks, 3 months and 6 months after surgery. Four tests including sharp-blunt discrimination, cold perception, pin prick sensation and electrical stimulation were performed.
    • RESULTS: Mean values of electrical stimulation were significantly higher 6 months after surgery (p < 0.05, on the other hand mean values of pin-prick sensation were significantly lower (p < 0.05. All patients regardless of the condition of greater palatine nerve were responsive to cold perception and sharp-blunt discrimination 6 months after surgery.
    • CONCLUSIONS: Following Le Fort I osteotomy, palatal  esponsiveness to electrical stimulation decreases and mechanical hyper sensitization occurs. Dissection of greater palatine nerve was shown to have no effect on the results.
    • KEYWORDS: Lefort I Osteotomy, Palatal Mocousa, Nerve Recovery.

  4. Exploding a myth: the capsule dehiscence mechanism and the function of pseudostomata in Sphagnum.

    Science.gov (United States)

    Duckett, Jeffrey G; Pressel, Silvia; P'ng, Ken M Y; Renzaglia, Karen S

    2009-01-01

    The nineteenth century air-gun explanation for explosive spore discharge in Sphagnum has never been tested experimentally. Similarly, the function of the numerous stomata ubiquitous in the capsule walls has never been investigated. Both intact and pricked Sphagnum capsules, that were allowed to dry out, all dehisced over an 8-12 h period during which time the stomatal guard cells gradually collapsed and their potassium content, measured by X-ray microanalysis in a cryoscanning electron microscope, gradually increased. By contrast, guard cell potassium fell in water-stressed Arabidopsis. The pricking experiments demonstrate that the air-gun notion for explosive spore discharge in Sphagnum is inaccurate; differential shrinkage of the capsule walls causes popping off the rigid operculum. The absence of evidence for a potassium-regulating mechanism in the stomatal guard cells and their gradual collapse before spore discharge indicates that their sole role is facilitation of sporophyte desiccation that ultimately leads to capsule dehiscence. Our novel functional data on Sphagnum, when considered in relation to bryophyte phylogeny, suggest the possibility that stomata first appeared in land plants as structures that facilitated sporophyte drying out before spore discharge and only subsequently acquired their role in the regulation of gaseous exchange.

  5. [Respiratory allergies among symptomatic bakers and pastry cooks: initial results of a prevalence study].

    Science.gov (United States)

    Bataille, A; Anton, M; Mollat, F; Bobe, M; Bonneau, C; Caramaniam, M N; Géraut, C; Dupas, D

    1995-01-01

    A survey was carried out on respiratory symptoms and skin prick response to common allergens, storage mite and occupational allergens. Among 178 symptomatics bakers and pastry workers from small businesses in western France, only 65 people underwent skin prick and specific-IgE. 12 (18%) workers were skin positive to at least one common or occupational allergens. The more often skin positive were D. Ptero. mite 36 (57%); Alpha amylase 23 (35%); wheat flour 17 (26%); saccharomyces cerevisiae 16 (25%); Ephestia 15 (24%). The sensitivity of skin test was better than specific IgE for D. Ptero. Mite 36 (57%); and Alpha amylase 23 (35%). The sensitivity of specific IgE was better than skin test for wheat flour 26 (45%) and rye flour 23 (40%). Occurrence of skin positive to occupational allergen among symptomatics with rhinitis and asthma is much more frequent in workers with skin positive to common allergens (40/36) than in workers with skin negative (8/20). Atopy must be regarded as an important predisposing factor for skin sensitisation to occupational allergens. We conclude in the necessity of a standardised allergologic exploration to be done in symptomatics bakers.

  6. Prevalence of food and airborne allergens in allergic patients in Kerman

    Directory of Open Access Journals (Sweden)

    Hamed Fouladseresht

    2014-07-01

    Full Text Available Background: Detection of various environmental allergens is the major challenge in allergic diseases and the only treatment is avoiding these allergens. The aim of this study was to determine the prevalence of food and airborne allergens in allergic patients using Skin Prick Test (SPT. Methods: A cross-sectional study was done on clinically confirmed patients of atopic-dermatitis (n=54, allergenic-rhinitis (n=64 and chronic-urticaria (n=39 who referred to asthma and allergy clinic at Afzali-Pour hospital in Kerman during 2008-2010. Skin prick test was done using allergen extracts to determine the patients' sensitivity to food and airborne antigens. Results: Fifty-nine percent of patients responded to at least one allergen. Allergy to airborne and food allergens was 55.9 % and 21.7%, respectively. Chenopodiaceae (22.9% and egg white (10.2% were most prevalent airborne and food allergens. Allergy to cockroach, egg white, egg yolk and tomato was significantly higher in males than in females (P<0.05. Conclusion: The results indicated that allergy to food and airborne allergens is different depending on the nutrition and environmental conditions.

  7. Fruit vinegars attenuate cardiac injury via anti-inflammatory and anti-adiposity actions in high-fat diet-induced obese rats.

    Science.gov (United States)

    Bounihi, Abdenour; Bitam, Arezki; Bouazza, Asma; Yargui, Lyece; Koceir, Elhadj Ahmed

    2017-12-01

    Fruit vinegars (FVs) are used in Mediterranean folk medicine for their hypolipidemic and weight-reducing properties. To investigate the preventive effects of three types of FV, commonly available in Algeria, namely prickly pear [Opuntia ficus-indica (L.) Mill (Cectaceae)], pomegranate [Punica granatum L. (Punicaceae)], and apple [Malus domestica Borkh. (Rosaceae)], against obesity-induced cardiomyopathy and its underlying mechanisms. Seventy-two male Wistar rats were equally divided into 12 groups. The first group served as normal control (distilled water, 7 mL/kg bw), and the remaining groups were respectively treated with distilled water (7 mL/kg bw), acetic acid (0.5% w/v, 7 mL/kg bw) and vinegars of pomegranate, apple or prickly pear (at doses of 3.5, 7 and 14 mL/kg bw, acetic acid content as mentioned above) along with a high-fat diet (HFD). The effects of the oral administration of FV for 18 weeks on the body and visceral adipose tissue (VAT) weights, plasma inflammatory and cardiac enzymes biomarkers, and in heart tissue were evaluated. Vinegars treatments significantly (p inflammatory and anti-adiposity properties of these vinegars.

  8. Zooming in on the lettuce genome: species relationships in Lactuca s.l., inferred from chromosomal and molecular characters

    OpenAIRE

    Koopman, W.J.M.

    2002-01-01

    Lactuca sativa (cultivated lettuce) is the world's most important leafy salad vegetable. Apart from L. sativa , the genus Lactuca contains ca. 75 wild species, potentially useful to improve, for example, taste, texture, and disease resistance of cultivated lettuce. The wild species L. serriola (Prickly Lettuce), L. saligna (Least Lettuce), and L. virosa (Great...

  9. MX Siting Investigation. DTN/OBTS Field Surveys. Volume III. Biological Resources Nevada and Utah.

    Science.gov (United States)

    1981-11-30

    Tumble mustard F MYCW Lepidium fremontii Desert pepperweed F CW,W CACTACEAE Echinocereus engelmannii Hedgehog cactus S MY Ferocactus acant’,jodes...Lepidium sp. Pepperweed F MS Sisymbrium altissimum Tumble mustard F Js Sisymbrium sp. Tumble mustard F BS CACTACEAE Echinocereus engelmannii Engelmannii...Streptanthella F BS,W lorostris StetnhlaTwist flower F PJ cordatus CACTACEAE Echinocereus Hedgehog cactus S BIS triglochidiatus Opuntia erinacea Prickly pear S PJ

  10. Allergic bronchopulmonary aspergillosis--a case report.

    Science.gov (United States)

    Chokhani, Ramesh; Neupane, Saraswoti; Kandel, Ishwar Sharma

    2004-12-01

    A 24 years old male presented with recurrent symptoms of cough and breathlessness for 6 years but increased in past 6 months. Fleeting radiological opacities, peripheral eosinophilia and central type bronchiectasus in high resolution CT scan gave the suspicion of allergic bronchopolmonary aspergilosis. Confirmation of the diagnosis was done by skin prick and immunological tests. The patient showed an excellent response to oral prednisolone.

  11. Occupational allergy to aquarium fish food: red midge larva, freshwater shrimp, and earthworm. A clinical and immunological study.

    Science.gov (United States)

    Meseguer Arce, J; Villajos, I M Sánchez-Guerrero; Iraola, V; Carnés, J; Fernández Caldas, E

    2013-01-01

    Chironomids seem to be the main cause of occupational allergy to aquarium fish food. The aim of this study was to investigate the pattern of occupational sensitization to 3 different arthropod species used as components of aquarium fish food. The study sample comprised 8 workers from a fish food packing department. The control group comprised 40 atopic patients (20 of whom were allergic to mites). We performed prick tests with extracts of red midge larva (Chironomus thummi), freshwater shrimp (Gammarus species), earthworm (Tubifex species), and other arthropod species and a battery of common inhalant allergens. We measured peak expiratory flow rate (PEFR) and specific immunoglobulin (Ig) E and performed a methacholine challenge test, nasal challenge test, and immunoblotting. Cross-reactivity analyses were completed using immunoblotting and CAP inhibition. Prick test results were positive to red midge larvae in 7 patients (87.5%), Gammarus in 5 (62.5%), Tubifex in 3 (37.5%), and mites in 6 (75%). In the mite-allergic controls, 30% had positive prick test results to red midge larvae. PEFR decreased > or = 20% during the packing process in all patients, and in 1 patient it indicated a dual asthmatic response. Methacholine challenge test results were positive in all participants. Nasal challenge tests were performed in 4 patients, and the results were positive. Specific IgE to red midge larvae was detected in 62.5%, Gammarus in 50%, and Tubifex in 16%. Bands of approximately 14-15 kDa and 31 kDa were observed in Gammarus and red midge larvae extracts. Cross-reactivity assays demonstrated that Gammarus totally inhibited red midge larvae, while Tubifex did so partially. Dermatophagoides pteronyssinus showed very low inhibitory capacity. Aquarium fish food arthropods are potent allergens with an elevated prevalence of sensitization and variable degree of crossreactivity. This is the first report of occupational allergy to Tubifex. More data are necessary to identify and

  12. High prevalence of lipid transfer protein sensitization in apple allergic patients with systemic symptoms.

    Directory of Open Access Journals (Sweden)

    Francisca Gomez

    Full Text Available Apple allergy manifests as two main groups of clinical entities reflecting different patterns of allergen sensitization: oral allergy syndrome (OAS and generalized symptoms (GS.We analysed the sensitization profile to a wide panel of different components of food allergens (rMal d 1, Mal d 2, rMal d 3, rMal d 4, rPru p 3, rBet v 1 and Pho d 2 for a population of Mediterranean patients with OAS and GS to apple.Patients (N = 81 with a history of apple allergy that could be confirmed by positive prick-prick test and/or double-blind-placebo-controlled food challenge (DBPCFC, were included. Skin prick test (SPT and ELISA were performed using a panel of inhalant, fruit and nut allergens. ELISA and ELISA inhibition studies were performed in order to analyse the sensitization patterns.Thirty-five cases (43.2% had OAS and 46 (56.8% GS. SPT showed a significantly higher number of positive results with peach, cherry and hazelnut in those with GS. ELISA showed a significantly high percentage of positive cases to rMal d 3, rMal d 4, rPru p 3 and Pho d 2 in patients with OAS and GS compared to controls, and to rBet v 1 in patients with OAS vs controls and between OAS and GS patients. Three different patterns of recognition were detected: positive to LTP (rMal d 3 or rPru p 3, positive to profilin (rMal d 4 and Pho d 2, or positive to both. There were also patients with rMal d 1 recognition who showed cross-reactivity to rBet v 1.In an apple allergy population with a high incidence of pollinosis different patterns of sensitization may occur. LTP is most often involved in those with GS. Profilin, though more prevalent in patients with OAS, has been shown to sensitise patients with both types of symptoms.

  13. Determinants of lung function and airway hyperresponsiveness in asthmatic children.

    Science.gov (United States)

    Bisgaard, H; Pedersen, S; Anhøj, J; Agertoft, L; Hedlin, G; Gulsvik, A; Bjermer, L; Carlsen, K H; Nordvall, L; Lundbäck, B; Wennergren, G; Werner, S; Bønnelykke, K; Weiss, S T

    2007-07-01

    Asthma patients exhibit an increased rate of loss of lung function. Determinants to such decline are largely unknown and the modifying effect of steroid therapy is disputed. This cross-sectional study aimed to elucidate factors contributing to such decline and the possible modifying effect of steroid treatment. We analyzed determinants of lung function and airway hyperresponsiveness (AHR) in a Scandinavian study of 2390 subjects from 550 families. Families were selected for the presence of two or more asthmatic children as part of a genetic study, Scandinavian Asthma Genetic Study (SAGA). The primary analysis studied the association between the lung function and delay of inhaled corticosteroids (ICS) after asthma diagnosis among asthmatic children and young adults with a history of regular ICS treatment (N=919). FEV(1) percent predicted (FEV(1)% pred) was 0.25% lower per year of delay from diagnosis until treatment (p=0.039). This association was significantly greater in allergy skin prick test negative children. There was no significant influence of gender, age at asthma onset, or smoking. In the secondary analysis of the whole population of 2390 asthmatics and non-asthmatics, FEV(1)% pred was inversely related to having asthmatic siblings (-7.9%; p<0.0001), asthma diagnosis (-2.7%; p=0.0007), smoking (-3.5%; p=0.0027), and positive allergy skin prick test (-0.47% per test; p=0.012), while positively related to being of female gender (1.8%; p=0.0029). Risk of AHR was higher by having asthmatic siblings (OR 2.7; p<0.0001), being of female gender (OR 2.0; p<0.0001), and having asthma (OR 2.0; p<0.0001). These data suggest that lung function is lower in asthmatics with delayed introduction of ICS therapy, smoking, and positive allergy skin prick test. Lung function is lower and AHR higher in female asthmatics and subjects with asthmatic siblings or established asthma.

  14. Sunflower seed allergy

    Science.gov (United States)

    Ukleja-Sokołowska, Natalia; Gawrońska-Ukleja, Ewa; Żbikowska-Gotz, Magdalena; Bartuzi, Zbigniew; Sokołowski, Łukasz

    2016-01-01

    Sunflower seeds are a rare source of allergy, but several cases of occupational allergies to sunflowers have been described. Sunflower allergens on the whole, however, still await precise and systematic description. We present an interesting case of a 40-year-old male patient, admitted to hospital due to shortness of breath and urticaria, both of which appeared shortly after the patient ingested sunflower seeds. Our laryngological examination revealed swelling of the pharynx with retention of saliva and swelling of the mouth and tongue. During diagnostics, 2 months later, we found that skin prick tests were positive to mugwort pollen (12/9 mm), oranges (6/6 mm), egg protein (3/3 mm), and hazelnuts (3/3 mm). A native prick by prick test with sunflower seeds was strongly positive (8/5 mm). Elevated concentrations of specific IgE against weed mix (inc. lenscale, mugwort, ragweed) allergens (1.04 IU/mL), Artemisia vulgaris (1.36 IU/mL), and Artemisia absinthium (0.49 IU/mL) were found. An ImmunoCap ISAC test found an average level of specific IgE against mugwort pollen allergen component Art v 1 - 5,7 ISU-E, indicating an allergy to mugwort pollen and low to medium levels of specific IgE against lipid transfer proteins (LTP) found in walnuts, peanuts, mugwort pollen, and hazelnuts. Through the ISAC inhibition test we proved that sunflower seed allergen extracts contain proteins cross-reactive with patients’ IgE specific to Art v 1, Art v 3, and Jug r 3. Based on our results and the clinical pattern of the disease we confirmed that the patient is allergic to mugwort pollen and that he had an anaphylactic reaction as a result of ingesting sunflower seeds. We suspected that hypersensitivity to sunflower LTP and defensin-like proteins, both cross-reactive with mugwort pollen allergens, were the main cause of the patient’s anaphylactic reaction. PMID:27222528

  15. Authorised allergen products for intracutaneous testing may no longer be available in Germany

    OpenAIRE

    Klimek, Ludger; Werfel, Thomas; Vogelberg, Christian; Jung, Kirsten

    2015-01-01

    Background: Beside the skin prick test, the intracutaneous test represents the most important skin test method for detecting type-1 allergies. With the incorporation of European directives into national law, test allergens used for allergy diagnosis are deemed medicinal products within the meaning of the German Medicinal Products Act (Arzneimittelgesetz) and therefore require marketing authorisation for distribution in Germany. The high costs of acquiring and maintaining these authorisations ...

  16. Hybridization rates between lettuce (Lactuca sativa) and its wild relative (L. serriola) under field conditions

    OpenAIRE

    D'Andrea, Luigi; Felber, François; Guadagnuolo, Roberto

    2008-01-01

    Hybridization and introgression between crops and wild relatives may have important evolutionary and ecological consequences such as gene swamping or increased invasiveness. In the present study, we investigated hybridization under field conditions between crop lettuce (Lactuca sativa) and its wild relative prickly lettuce (L. serriola), two cross-compatible, predominantly autogamous and insect pollinated species. In 2003 and 2004, we estimated the rates of hybridization between L. sativa and...

  17. Neuroticism, anxiety, and depression in Egyptian atopic bronchial asthma

    OpenAIRE

    Amal Fathy; Taha t Abd Algawad; Eman O. Arram; Hala Elboraei; Manal S. Arafat; Sherin S. Elmetwaly

    2014-01-01

    The association between allergic and psychological disorders had been reported, but whether the key mediating ingredients are predominantly biological, psychological, or mere artifacts remains unknown. We aim to examine the relationship between objectively measured atopic status and anxiety, depression, and neuroticism. Methods: This randomized case controlled trial was conducted on 50 atopic patients and 50 healthy controls. Atopy was determined by skin prick test and allergy related symp...

  18. Cow?s milk allergy: evidence-based diagnosis and management for the practitioner

    OpenAIRE

    Lifschitz, Carlos; Szajewska, Hania

    2014-01-01

    This review summarizes current evidence and recommendations regarding cow?s milk allergy (CMA), the most common food allergy in young children, for the primary and secondary care providers. The diagnostic approach includes performing a medical history, physical examination, diagnostic elimination diets, skin prick tests, specific IgE measurements, and oral food challenges. Strict avoidance of the offending allergen is the only therapeutic option. Oral immunotherapy is being studied, but it is...

  19. A Type-II First Branchial Cleft Anomaly Presenting as a Post-Auricular Salivary Fistula: A Rare Presentation.

    Science.gov (United States)

    Jain, S; Deshmukh, Pt; Gupta, M; Shukla, S

    2014-01-01

    First branchial cleft anomalies are rare with the average age of presentation as 19 years. There is an average delay of 3.5 years between initial presentation and adequate treatment due to diagnostic dilemma. A very rare variant of first branchial cleft anomaly presenting as a post-auricular salivary fistula is reported. A 12-year-old girl presented with a history of intermittent watery discharge, more so at the time of meals from a right post-auricular opening for last 3 years. Computed tomography sialography revealed a fistulous tract connecting the sub segmental duct of the parotid gland extending along the pre-tragus region in subcutaneous plane up to mastoid tip after passing inferior to external auditory canal. Superficial parotidectomy with identification of facial nerve branches was carried out for excision of the tract. Histopathology revealed sinus tract comprising of ectodermal components and acini of the parotid gland. We classified our case into work's type-2 based on anatomical location at an angle of mandible, its relationship to parotid gland and facial nerve and previous history of ear discharge. To the best of our knowledge, this is the first case of its type to be reported.

  20. A Type-II First Branchial Cleft Anomaly Presenting as a Post-Auricular Salivary Fistula: A Rare Presentation

    Science.gov (United States)

    Jain, S; Deshmukh, PT; Gupta, M; Shukla, S

    2014-01-01

    First branchial cleft anomalies are rare with the average age of presentation as 19 years. There is an average delay of 3.5 years between initial presentation and adequate treatment due to diagnostic dilemma. A very rare variant of first branchial cleft anomaly presenting as a post-auricular salivary fistula is reported. A 12-year-old girl presented with a history of intermittent watery discharge, more so at the time of meals from a right post-auricular opening for last 3 years. Computed tomography sialography revealed a fistulous tract connecting the sub segmental duct of the parotid gland extending along the pre-tragus region in subcutaneous plane up to mastoid tip after passing inferior to external auditory canal. Superficial parotidectomy with identification of facial nerve branches was carried out for excision of the tract. Histopathology revealed sinus tract comprising of ectodermal components and acini of the parotid gland. We classified our case into work's type-2 based on anatomical location at an angle of mandible, its relationship to parotid gland and facial nerve and previous history of ear discharge. To the best of our knowledge, this is the first case of its type to be reported. PMID:24669347

  1. Repair for Congenital Macrostomia: Vermilion Square Flap Method

    Directory of Open Access Journals (Sweden)

    Renuka Dhingra

    2014-01-01

    Full Text Available Transverse facial clefts (macrostomia are rare disorders that result when the embryonic mandibular and maxillary processes of the first branchial arch fail to fuse due to failure of mesodermal migration and merging to obliterate the embryonic grooves between the maxillary and mandibular processes to form the angle of the mouth at its normal anatomic position. Macrostomia may be seen alone or in association with other anomalies. It may be unilateral, extending along a line from the commissure to the tragus or bilateral. It is usually partial but rarely complete. Transverse facial clefts are more common in males and more common on the left side when unilateral. The goal of macrostomia reconstruction is to achieve functional, symmetrical, and accurate oral commissure with minimal scar. In this paper, we present a six-year-old girl with unilateral macrostomia with preauricular skin tags and malformation of pinna on ipsilateral side treated with vermillion-square flap method. The scar is placed at the upper lip. At two-month followup, the oral commissures are symmetric, the scars are inconspicuous, and the overall balance of facial contour and lip is excellent. We recommend this method for patients with mild to moderate macrostomia.

  2. Fire Makers, Barnyards, and Prickly Forests: A Preschool Stroll around the Block

    Science.gov (United States)

    Bentley, Dana Frantz

    2012-01-01

    The daily trips outside can be challenging, and living in Manhattan makes them even more complex. It seems there is no such thing as a simple stroll, or a quick trip outside; and the winter weather makes such excursions that much more complex. Yet, it is always worth it. In the author's nine years of teaching, she has discovered that a "quick…

  3. An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting.

    Science.gov (United States)

    Jaganath, Balusamy; Subramanyam, Kondeti; Mayavan, Subramanian; Karthik, Sivabalan; Elayaraja, Dhandapani; Udayakumar, Rajangam; Manickavasagam, Markandan; Ganapathi, Andy

    2014-05-01

    An efficient and reproducible Agrobacterium-mediated in planta transformation was developed in Jatropha curcas. The various factors affecting J. curcas in planta transformation were optimized, including decapitation, Agrobacterium strain, pin-pricking, vacuum infiltration duration and vacuum pressure. Simple vegetative in vivo cleft grafting method was adopted in the multiplication of transformants without the aid of tissue culture. Among the various parameters evaluated, decapitated plants on pin-pricking and vacuum infiltrated at 250 mmHg for 3 min with the Agrobacterium strain EHA 105 harbouring the binary vector pGA 492 was proved to be efficient in all terms with a transformation efficiency of 62.66%. Transgene integration was evinced by the GUS histochemical analysis, and the GUS positive plants were subjected to grafting. Putatively transformed J. curcas served as "Scion" and the wild type J. curcas plant severed as "Stock". There was no occurrence of graft rejection and the plants were then confirmed by GUS histochemical analysis, polymerase chain reaction (PCR) and Southern hybridization. Genetic stability of the grafted plants was evaluated by using randomly amplified polymorphic DNA (RAPD), marker which showed 100% genetic stability between mother and grafted plants. Thus, an efficient in planta transformation and grafting based multiplication of J. curcas was established.

  4. Component-resolved diagnostics in vernal conjunctivitis.

    Science.gov (United States)

    Armentia, Alicia; Sanchís, Eugenia; Montero, Javier A

    2016-10-01

    Conventional diagnostic tests in allergy are insufficient to clarify the cause of vernal conjunctivitis. Component-resolved diagnostic (CRD) by microarray allergen assay may be useful in detecting allergens that might be involved in the inflammatory process. In a recent trial in patients suffered from eosinophilic esophagitis, after 2 years of the CRD-guided exclusion diet and specific immunotherapy, significant clinical improvement was observed, and 68% of patients were discharged (cure based on negative biopsy, no symptoms, and no medication intake). Our new objective was to evaluate IgE-mediated hypersensitivity by CRD in tears and serum from patients with vernal conjunctivitis and treat patients with identified triggering allergens by specific immunotherapy. Twenty-five patients with vernal conjunctivitis were evaluated. The identified triggering allergens were n Lol p 1 (11 cases), n Cyn d 1 (eight cases), group 4 and 6 grasses (six cases) and group 5 of grasses (five cases). Prick test and pollen IgE were positive in one case. Clinical improvement was observed in 13/25 vernal conjunctivitis patients after 1-year specific immunotherapy. CRD seems to be a more sensitive diagnostic tool compared with prick test and IgE detection. Specific CRD-led immunotherapy may achieve clinical improvements in vernal conjunctivitis patients.

  5. Looking for immunotolerance: a case of allergy to baker's yeast (Saccharomyces cerevisiae).

    Science.gov (United States)

    Pajno, G B; Passalacqua, G; Salpietro, C; Vita, D; Caminiti, L; Barberio, G

    2005-09-01

    We describe one case of baker's yeast true allergy in a boy with previously diagnosed mite-allergy and atopic dermatitis. At the age of 6, being atopic dermatitis and rhinitis well controlled by drugs, he began to experience generalized urticaria and asthma after eating pizza and bread, but only fresh from the oven. The diagnostic workup revealed single sensitization to baker's yeast (Saccharomyces cerevisiae), and a severe systemic reaction also occurred during the prick-by-prick procedure. After discussing with parents, no special dietary restriction was suggested but the use of autoinjectable adrenaline and on demand salbutamol. A diary of symptoms was recorded by means of a visual-analog scale. During the subsequent 2 years, the severity of symptoms was progressively reduced, and presently urticaria has disappeared. Only cough persists, invariantly after eating just-baked and yeast-containing foods. If bread, pizza and cakes are ate more than one hour after preparation, no symptom occur at all. Baker's yeast is a common component of everyday diet and it usually acts as an allergen only by the inhalatory route. We speculate that the continuous exposure to saccharomyces in foods may have lead to an immunotolerance with a progressive reduction of symptoms, whereas why the allergens is active only in ready-baked foods remains unexplained.

  6. [Occupational allergy in health personnel].

    Science.gov (United States)

    Larese Filon, Francesca; Bagnato, Emma

    2003-01-01

    Health care workers are exposed to many agents that can cause irritant or allergic contact dermatitis. In nurses with eczema of the hands latex sensitivity can play an important role in the occurrence of urticaria, rhinitis and asthma. To determine the prevalence of irritant and allergic contact dermatitis and contact urticaria and the role of skin sensitization to common and occupational haptens and allergens in a group of health care workers with skin problems. Retrospective review of 204 health care workers assessed by prick and patch testing in an occupational health clinic. The diagnoses included 35.3% with irritant contact dermatitis, 64.7% with allergic contact dermatitis and 7.3% with contact urticaria to latex. Three workers complained of asthma and 5 complained of rhinitis related to latex sensitization. At present 12.9% of atopic subjects were sensitized to latex by skin prick against 21.9% in 1998, so sensitization showed a decline in the years considered. Contact dermatitis and sensitization to natural rubber latex is a significant problem and nurses should be tested for both types of hypersensitivity, as well as being patch tested to standard, rubber and disinfectants series. The need is stressed for preventive measures to prevent the onset of contact dermatitis and to avoid latex exposure.

  7. Sensitization of children in the Stockholm area to house dust mites.

    Science.gov (United States)

    Nordvall, S L; Eriksson, M; Rylander, E; Schwartz, B

    1988-09-01

    Atopic sensitization of children in the Stockholm area to house dust mites (HDM) was investigated in a case-control study. Sixty children with and 60 without positive skin prick tests for HDM were matched for age and sex. HDM-sensitized children had previously more often lived in other areas known to be mite infested than the control children. Sensitization to mites was related to dampness in the homes, but no significant relationship was found to the type of residence, frequent visits to a summer house in the archipelago or parental smoking. Dust samples from mattresses of the children with the strongest positive reactions to mites in skin prick tests and the respective controls were subjected to an enzyme immunoassay, to measure the content of the major allergens of the Dermatophagoides (D.) species D. pterinyssinus, D. farinae and D. microceras. Mattress dust samples from the beds of HDM-sensitized children contained significantly higher HDM antigen concentrations than those from the beds of controls. Private houses contained significantly more HDM antigens than flats and 10 of 11 homes in which a dampness problem was recognized contained mite antigens. It is postulated that mite infestation is increasing in the area, energy-saving measures creating improved conditions for HDM survival.

  8. Host plant adaptation in Drosophila mettleri populations.

    Directory of Open Access Journals (Sweden)

    Sergio Castrezana

    Full Text Available The process of local adaptation creates diversity among allopatric populations, and may eventually lead to speciation. Plant-feeding insect populations that specialize on different host species provide an excellent opportunity to evaluate the causes of ecological specialization and the subsequent consequences for diversity. In this study, we used geographically separated Drosophila mettleri populations that specialize on different host cacti to examine oviposition preference for and larval performance on an array of natural and non-natural hosts (eight total. We found evidence of local adaptation in performance on saguaro cactus (Carnegiea gigantea for populations that are typically associated with this host, and to chemically divergent prickly pear species (Opuntia spp. in a genetically isolated population on Santa Catalina Island. Moreover, each population exhibited reduced performance on the alternative host. This finding is consistent with trade-offs associated with adaptation to these chemically divergent hosts, although we also discuss alternative explanations for this pattern. For oviposition preference, Santa Catalina Island flies were more likely to oviposit on some prickly pear species, but all populations readily laid eggs on saguaro. Experiments with non-natural hosts suggest that factors such as ecological opportunity may play a more important role than host plant chemistry in explaining the lack of natural associations with some hosts.

  9. Prevalence and clinical impact of sensitization to latex and fruits in dentistry students at the University of Antioquia, and its relationship with allergy to fruits

    Directory of Open Access Journals (Sweden)

    Echenique Manrique, Alejandro

    2015-04-01

    Full Text Available Objective: To determine the prevalence and clinical impact of sensitization to latex and to five tropical fruits (banana, avocado, kiwi, pineapple and passion fruit in dentistry students. Methods: Analytical cross-sectional study of 128 dentistry students at University of Antioquia in Medellín, Colombia. Information was collected by means of a questionnaire and skin prick tests with latex and fruits were done. Results: All students reported having had contact with latex. Nine of them informed at least one episode of adverse reaction to contact with latex without proof of sensitization to it. Five reported at least one reaction with one of the fruits, but skin prick tests were negative. Four of the 14 students who reported gastrointestinal symptoms were sensitized to latex or to one of the tested fruits. Overall, latex sensitization rate was 3.1%. Conclusion: This percentage of sensitization to latex is lower than that in other studies; this may be due to the expression of immune mechanisms other than IgE mediation. We failed to demonstrate a higher sensitization rate to latex as students advanced in their career. The association between gastrointestinal symptoms and sensitization to both fruit and latex is to be emphasized.

  10. Allergic anaphylaxis due to subcutaneously injected heparin

    Directory of Open Access Journals (Sweden)

    Anders Diana

    2013-01-01

    Full Text Available Abstract Heparins are one of the most used class of anticoagulants in daily clinical practice. Despite their widespread application immune-mediated hypersensitivity reactions to heparins are rare. Among these, the delayed-type reactions to s.c. injected heparins are well-known usually presenting as circumscribed eczematous plaques at the injection sites. In contrast, potentially life-threatening systemic immediate-type anaphylactic reactions to heparins are extremely rare. Recently, some cases of non-allergic anaphylaxis could be attributed to undesirable heparin contaminants. A 43-year-old patient developed severe anaphylaxis symptoms within 5–10 minutes after s.c. injection of enoxaparin. Titrated skin prick testing with wheal and flare responses up to an enoxaparin dilution of 1:10.000 indicated a probable allergic mechanism of the enoxaparin-induced anaphylaxis. The basophil activation test as an additional in-vitro test method was negative. Furthermore, skin prick testing showed rather broad cross-reactivity among different heparin preparations tested. In the presented case, history, symptoms, and results of skin testing strongly suggested an IgE-mediated allergic hypersensitivity against different heparins. Therefore, as safe alternative anticoagulants the patient could receive beneath coumarins the hirudins or direct thrombin inhibitors. Because these compounds have a completely different molecular structure compared with the heparin-polysaccharides.

  11. Basophil activation test: food challenge in a test tube or specialist research tool?

    Science.gov (United States)

    Santos, Alexandra F; Lack, Gideon

    2016-01-01

    Oral food challenge (OFC) is the gold-standard to diagnose food allergy; however, it is a labour and resource-intensive procedure with the risk of causing an acute allergic reaction, which is potentially severe. Therefore, OFC are reserved for cases where the clinical history and the results of skin prick test and/or specific IgE do not confirm or exclude the diagnosis of food allergy. This is a significant proportion of patients seen in Allergy clinics and results in a high demand for OFC. The basophil activation test (BAT) has emerged as a new diagnostic test for food allergy. With high diagnostic accuracy, it can be particularly helpful in the cases where skin prick test and specific IgE are equivocal and may allow reducing the need for OFC. BAT has high specificity, which confers a high degree of certainty in confirming the diagnosis of food allergy and allows deferring the performance of OFC in patients with a positive BAT. The diagnostic utility of BAT is allergen-specific and needs to be validated for different allergens and in specific patient populations. Standardisation of the laboratory methodology and of the data analyses would help to enable a wider clinical application of BAT.

  12. Proporción divina en pacientes con síndrome de clase II división 1 Divine proportion in patients with Class II Division I Syndrome

    Directory of Open Access Journals (Sweden)

    Yaima Lazo Amador

    2012-06-01

    Full Text Available La belleza y la armonía facial desempeñan una función decisiva en las relaciones sociales del hombre. La afectación estética es el principal motivo de consulta de los pacientes de Ortodoncia. La Proporción Divina está siendo estudiada cada día más, con fines diagnósticos y terapéuticos relacionados con la estética facial. Con el objetivo de evaluar el comportamiento de la Proporción Divina en mediciones faciales en pacientes con Síndrome de Clase II división 1 y su relación con el sexo se realizó un estudio descriptivo transversal durante los años 2008-2010 en el Departamento de Ortodoncia de la Facultad de Estomatología de la Habana. La muestra estuvo formada por 30 pacientes que cumplieron con los criterios de inclusión y se estudiaron las mediciones faciales mediante fotografías de frente y perfil. El estudio arrojó que los mayores porcentajes se encontraron en los pares que relacionaban la distancia entre el canto lateral de los ojos y el margen lateral de la nariz (66,7 %; la base del tragus y el canto lateral del ojo y de este a la punta de la nariz (66,7 %; de trichion al ala de la nariz y de esta al mentón (63,3 %. Pocos pacientes mostraron la Proporción Divina en las mediciones faciales estudiadas, sin diferencias significativas en su comportamiento en relación con el sexo.The beauty and the facial harmony have a decisive function in social relationships of man. The esthetic affection is the leading cause of consultation of patients to Orthodontics. Divine Proportion is being more and more studied having diagnostic and therapeutical aims related to the facial aesthetics. To assess the behavior of Divine Proportion in facial measurements in patients presenting with Class II Division I syndrome and its relation to sex a cross-sectional and descriptive study was conducted for 2008-2010 in the Orthodontics Department of the Stomatology Faculty of La Habana. The sample included 30 patients who fulfilled the inclusion

  13. Sensitivity to the Main Allergens in Children with Allergic Diseases

    Directory of Open Access Journals (Sweden)

    O.D. Kuznietsova

    2015-11-01

    Objective of the research — to study hypersensitivity to the main allergens in children with allergic diseases based on the results of skin allergy testing, as well as to analyze the structure of diseases. Materials and methods. We have examined 228 children using skin prick testing, the estimation of results was conducted 25–40 minutes after performing the test. Associations between the results of skin prick test with various allergens were studied using cross-correlation analysis in the package of applied statistics Statistics 6.0. Results. 85.5 % of children were sensitized to the pollen allergens, domestic — 54 %, food — 21 %, fungal allergens — 35 %. Among pollen plants, there prevails sensitization to ambrosia — 47.8 %, sunflower — 49.5 %, cyclachaena — 38.5 %; among domestic allergens — to the tick species D.рteronyssinus and D.farinae — 24 %, cat hair — 19.7 %, among fungal — to Alternaria (23 %. Most often hyperergic reaction (papule diameter ≥ 8 mm was observed to cyclachaena (44 %, sunflower (46 %, ambrosia (50 %, cat hair (42 %, D.farinae (39 %. We have established significant (р < 0.05 correlations of mainly middle strength between positive prick-tests in pairs: ambrosia — cyclachaena (r = +0.43, ambrosia — sunflower (r = +0.43, acarus D.рteronyssinus — D.farinae (r = +0.66, mixture «birch, alder, oak, hazel» — ryegrass (r = +0.53, beef meat — egg yolk (r = +0.42, pork meat — chicken meat (r = +0.35, milk (r = +0.36, wool of sheep — pork (r = +0.36. Conclusion. Predominance of sensitization to pollen allergens represents the epidemiological situation in the South region of Ukraine. The presence of correlations between the different types of allergens indicates the cross reactions between them. In case of multiple positive results of skin allergen tests, the study using molecular allergy diagnostic method is recommended to establish genuine or cross allergy.

  14. Extracción y caracterización química y reológica de hidrocoloide de opuntia spinulifera con perspectivas de aditivo en la industria de alimentos

    OpenAIRE

    Vargas Rodríguez, Lorena

    2012-01-01

    The hydrocolloid at cladodes of Opuntia, is a molecule of secondary metabolism, it´s little known about their physical and chemical properties. The study developed in this work contributes to reassess the genus Opuntia (prickly pear) as a source for obtaining from additive at the food industry. In this work were established a methodology of extraction, which involved the optimization of several parameters of the raw material stage to extract. The Opuntia spinulifera was selected due to their ...

  15. Wetlands Research Program. Corps of Engineers Wetlands Delineation Manual. Appendix C. Section 1. Region O - California.

    Science.gov (United States)

    1987-01-01

    status is questioned. An X prior to the species name in the scientific name column denotes a hybrid . For purposes of this manual, all species appearing...S. Wats. Rusty molly kochia FACW K. scc’pari.a (L.) Schrader Sunmmer cypress ...actuca pu~ZchelZa (Pursh) DC. Chicory lettuce -serriola L. Prickly... lettuce FAG :antana cwnara L. Lantana% :asthenia burkei (Greene) Greene Gold-fields chry8Sstoma (Fisch. & C. A. Lasthenia Meyer) Greene Lconjtgen8 Greene

  16. Food allergy: a clinician's criteria for including sera in a serum bank.

    Science.gov (United States)

    Ballmer-Weber, B K; Fernández-Rivas, M

    2008-10-01

    Safety assessment for genetically-engineered crop plants includes assessment for allergic responses. To facilitate this assessment, serum banks should contain well-characterised sera from patients with confirmed food allergies. A serum is defined as well-characterised if it is taken from a patient who has a convincing history of allergic responses to a known allergen or an allergen-containing food, a positive skin prick test (or elevated IgE response), and a positive response in a clinical food challenge.

  17. Penyelenggaraan Hygiene Sanitasi Pengolahan Salak di PT. Agrina Desa Parsalakan Kecamatan Angkola Barat Kabupaten Tapanuli Selatan Tahun 2013

    OpenAIRE

    Siregar, Yuli Arisyah; Dharma, Surya; Hasan, Wirsal

    2013-01-01

    Operation Of Hygiene Sanitation Salak Processing At PT. Agrina Parsalakan Village West Angkola Subdistrict South Tapanuli District In 2013. Efforts to control dietary factors, people, places and equipment that may or may cause illness or health problems, it is necessary hygiene sanitation in any food processing in order to avoid contamination. Salak (Salacca edulis) is a plant which its stalk close by midrib leaves, prickly on midrib and stems leaves, fleshy white fruit, blackish brown hard s...

  18. Book Reviews

    OpenAIRE

    Redactie KITLV

    2004-01-01

    -Bill Maurer, Mimi Sheller, Consuming the Caribbean: From Arawaks to Zombies. New York: Routledge, 2003. ix + 252 pp. -Norman E. Whitten, Jr., Richard Price ,The root of roots: Or, how Afro-American anthropology got its start. Chicago: Prickly Paradigm Press/University of Chicago Press, 2003. 91 pp., Sally Price (eds) -Holly Snyder, Paolo Bernardini ,The Jews and the expansion of Europe to the West, 1450-1800. New York: Berghahn Books, 2001. xv + 567 pp., Norman Fiering (eds) -Bridget Brereto...

  19. Nopal Cactus (Opuntia ficus-indica) as a Source of Bioactive Compounds for Nutrition, Health and Disease

    OpenAIRE

    Karym El-Mostafa; Youssef El Kharrassi; Asmaa Badreddine; Pierre Andreoletti; Joseph Vamecq; M'Hammed Saïd El Kebbaj; Norbert Latruffe; Gérard Lizard; Boubker Nasser; Mustapha Cherkaoui-Malki

    2014-01-01

    Opuntia ficus-indica, commonly referred to as prickly pear or nopal cactus, is a dicotyledonous angiosperm plant. It belongs to the Cactaceae family and is characterized by its remarkable adaptation to arid and semi-arid climates in tropical and subtropical regions of the globe. In the last decade, compelling evidence for the nutritional and health benefit potential of this cactus has been provided by academic scientists and private companies. Notably, its rich composition in polyphenols, vit...

  20. Papain-induced occupational rhinoconjunctivitis and asthma – A case report

    OpenAIRE

    Tymoszuk, Diana; Wiszniewska, Marta; Walusiak-Skorupa, Jolanta

    2016-01-01

    This report presents a case of occupational asthma, rhinitis and conjunctivitis to papain in a 50-year-old herbs and spices packer, with documented increased eosinophilia in induced sputum and in the nasal lavage fluids after a specific inhalation challenge test (SICT) and specific nasal challenge test (SNCT) with this enzyme. Immunoglobulin E-mediated (IgE) sensitization to papain was confirmed by positive results of a skin prick test with specific solution. Specific inhalation and nasal cha...

  1. Bidirectional recovery patterns of Mojave Desert vegetation in an aqueduct pipeline corridor after 36 years: I. Perennial shrubs and grasses

    Science.gov (United States)

    Berry, Kristin H.; Weigand, James F.; Gowan, Timothy A.; Mack, Jeremy S.

    2015-01-01

    We studied recovery of 21 perennial plant species along a severely disturbed aqueduct corridor in a Larrea tridentata-Ambrosia dumosa plant alliance in the Mojave Desert 36 years after construction. The 97-m wide corridor contained a central dirt road and buried aqueduct pipeline. We established transects at 0 m (road verge), 20 m and 40 m into the disturbance corridor, and at 100 m in undisturbed habitat (the control). Although total numbers of shrubs per transect did not vary significantly with distance from the verge, canopy cover of shrubs, species richness, and species diversity were higher in the control than at the verge and other distances. Canopy cover of common shrubs (Ericameria nauseosa, Ambrosia salsola, A. dumosa, L. tridentata, Grayia spinosa) and perennial grasses (Elymus elymoides, Poa secunda) also varied significantly by location. Discriminant analysis clearly separated the four distances based on plant composition. Patterns of recovery were bidirectional: secondary succession from the control into the disturbance corridor and inhibition from the verge in the direction of the control. Time estimated for species composition to resemble the control is dependent on location within the disturbance corridor and could be centuries at the road verge. Our findings have applications to other deserts.

  2. Soil amendment with halophytes induces physiological changes and reduces root-knot infection in eggplant and okra

    Directory of Open Access Journals (Sweden)

    Waseem M. ABBASI

    2011-01-01

    Full Text Available Root-knot nematode, Meloidogyne javanica (Treub Chitwood is a soil-borne plant pathogen of roots. Nematode infection results in altered plant growth and physicochemical processes due to gall formation. Many plants contain unique biochemicals that have biocidal properties and offer a potential novel approach to suppress the nematode populations in soil and improve growth of crop plants. In the present study effect of some indigenous halophytic plant species (Tamarix indica Willd, Suaeda fruticosa Forssk and Salsola imbricata (Schultz Dandy were tested against M. javanica. Tested halophytes significantly (P<0.001 reduced egg hatching and caused mortality of second stage juveniles (J2 in vitro. These halophytes when incorporated in soil (0.3, 0.5 and 1% w/w markedly increased growth of eggplant (Solanum melongena L. cv. Black beauty and okra (Abelmoschus esculentus [L.] Moench. cv. Arka anamika and provided control of root-knot infection at higher doses (0.5 and 1%. Amended eggplants and okra showed significant (P<0.001 increase in chlorophylls and decrease in chlorophyll a/b ratio. Protein concentration in leaves of both the plants were increased with 1% amendment of S. fruticosa and S. imbricata. While nucleic acid concentrations were varied with different treatments.  

  3. Effects of high fire frequency in creosote bush scrub vegetation of the Mojave Desert

    Science.gov (United States)

    Brooks, M.L.

    2012-01-01

    Plant invasions can increase fire frequency in desert ecosystems where fires were historically infrequent. Although there are many resource management concerns associated with high frequency fire in deserts, fundamental effects on plant community characteristics remain largely unstudied. Here I describe the effects of fire frequency on creosote bush scrub vegetation in the Mojave Desert, USA. Biomass of the invasive annual grass Bromus rubens L. increased following fire, but did not increase further with additional fires. In contrast, density, cover and species richness of native perennial plants each decreased following fire and continued to decrease with subsequent fires, although not as dramatically as after the initial fire. Responses were similar 5 and 14 years post-fire, except that cover of Hymenoclea salsola Torr. & A. Gray and Achnatherum speciosa Trin. & Rupr. both increased in areas burnt once. These results suggest that control of B. rubens may be equally warranted after one, two or three fires, but revegetation of native perennial plants is most warranted following multiple fires. These results are valid within the scope of this study, which is defined as relatively short term vegetation responses (???14 years) to short fire return intervals (6.3 and 7.3 years for the two and three fire frequency levels) within creosote bush scrub of the Mojave Desert. ?? 2012 IAWF.

  4. Clinical relevance is associated with allergen-specific wheal size in skin prick testing

    DEFF Research Database (Denmark)

    Haahtela, T.; Burbach, G. J.; Bachert, C.

    2014-01-01

    , asthma, atopic dermatitis, food allergy). The effects of age, gender, and geographical area on SPT results were assessed. For each allergen, the wheal size in mm with an 80% positive predictive value (PPV) for being clinically relevant was calculated. ResultsDepending on the allergen, from 40% (blatella...... by providing quantitative decision points. MethodsThe GA(2)LEN SPT study with 3068 valid data sets was used to investigate the relationship between SPT results and patient-reported clinical relevance for each of the 18 inhalant allergens as well as SPT wheal size and physician-diagnosed allergy (rhinitis...... SPT reactions had a smaller risk of sensitizations being clinically relevant compared with adults. The 80% PPV varied from 3 to 10mm depending on the allergen. ConclusionThese reading keys' for 18 inhalant allergens can help interpret SPT results with respect to their clinical significance. A SPT form...

  5. Resolving a Prickly Situation: Involving Stakeholders in Invasive Cactus Management in South Africa.

    Science.gov (United States)

    Novoa, Ana; Kaplan, Haylee; Wilson, John R U; Richardson, David M

    2016-05-01

    The regulation and management of alien species can be contentious, particularly when the stakeholders who benefit from alien species are different from those who suffer the costs. We propose a consultative process involving relevant stakeholders in invasive species management decisions. The process involves (1) the identification of relevant stakeholders, (2) assessing their perceptions, (3) enhancing interaction between stakeholders, (4) assessing changes in stakeholders' perceptions following interactions with other stakeholders, and (5) developing management recommendations in collaboration with stakeholders. We demonstrate the application of the process using the family Cactaceae ('cacti') in South Africa. Many species of cacti have been introduced to the country over the past two centuries, mostly for horticulture, food and fodder, and hundreds of other species have been introduced in the past few decades (or are likely to be introduced soon) for horticulture. Using the proposed process enabled the negotiation and participation of all stakeholders in decision making and helped minimize contentious situations by clarifying stakeholder's beliefs and exploring consensus solutions. Consequently, management objectives were broadly supported by all stakeholders. These results will be included in a national cactus management strategy for South Africa.

  6. Prickly business: abundance of sea urchins on breakwaters and coral reefs in Dubai.

    Science.gov (United States)

    Bauman, Andrew G; Dunshea, Glenn; Feary, David A; Hoey, Andrew S

    2016-04-30

    Echinometra mathaei is a common echinoid on tropical reefs and where abundant plays an important role in the control of algal communities. Despite high prevalence of E. mathaei on southern Persian/Arabian Gulf reefs, their abundance and distribution is poorly known. Spatial and temporal patterns in population abundance were examined at 12 sites between breakwater and natural reef habitats in Dubai (UAE) every 3 months from 2008 to 2010. Within the breakwater habitat, densities were greatest at shallow wave-exposed sites, and reduced with both decreasing wave-exposure and increasing depth. Interestingly, E. mathaei were significantly more abundant on exposed breakwaters than natural reef sites, presumably due to differences in habitat structure and benthic cover. Population abundances differed seasonally, with peak abundances during summer (July-September) and lower abundances in winter (December-February). Seasonal fluctuations are likely the result of peak annual recruitment pulses coupled with increased fish predation from summer to winter. Copyright © 2015 Elsevier Ltd. All rights reserved.

  7. A guide to performing skin-prick testing in practice: tips and tricks of ...

    African Journals Online (AJOL)

    2013-04-16

    Apr 16, 2013 ... individuals to produce specific immunoglobulin E antibodies to environmental ... pollens and moulds.1,3,6-9 Common food allergens in children include cow's milk .... Cats, dogs and other animals with fur. Inhalant allergens:.

  8. Performance evaluation of the microINR® point-of-care INR-testing system.

    Science.gov (United States)

    Joubert, J; van Zyl, M C; Raubenheimer, J

    2018-04-01

    Point-of-care International Normalised Ratio (INR) testing is used frequently. We evaluated the microINR ® POC system for accuracy, precision and measurement repeatability, and investigated instrument and test chip variability and error rates. Venous blood INRs of 210 patients on warfarin were obtained with Thromborel ® S on the Sysmex CS-2100i ® analyser and compared with capillary blood microINR ® values. Precision was assessed using control materials. Measurement repeatability was calculated on 51 duplicate finger-prick INRs. Triplicate finger-prick INRs using three different instruments (30 patients) and three different test chip lots (29 patients) were used to evaluate instrument and test chip variability. Linear regression analysis of microINR ® and Sysmex CS2100i ® values showed a correlation coefficient of 0.96 (P < .0001) and a positive proportional bias of 4.4%. Dosage concordance was 93.8% and clinical agreement 95.7%. All acceptance criteria based on ISO standard 17593:2007 system accuracy requirements were met. Control material coefficients of variation (CV) varied from 6.2% to 16.7%. The capillary blood measurement repeatability CV was 7.5%. No significant instrument (P = .93) or test chip (P = .81) variability was found, and the error rate was low (2.8%). The microINR ® instrument is accurate and precise for monitoring warfarin therapy. © 2017 John Wiley & Sons Ltd.

  9. Lettuce contact allergy.

    Science.gov (United States)

    Paulsen, Evy; Andersen, Klaus E

    2016-02-01

    Lettuce (Lactuca sativa L.) and its varieties are important vegetable crops worldwide. They are also well-known, rarely reported, causes of contact allergy. As lettuce allergens and extracts are not commercially available, the allergy may be underdiagnosed. The aims of this article are to present new data on lettuce contact allergy and review the literature. Lettuce is weakly allergenic, and occupational cases are mainly reported. Using aimed patch testing in Compositae-allergic patients, two recent Danish studies showed prevalence rates of positive lettuce reactions of 11% and 22%. The majority of cases are non-occupational, and may partly be caused by cross-reactivity. The sesquiterpene lactone mix seems to be a poor screening agent for lettuce contact allergy, as the prevalence of positive reactions is significantly higher in non-occupationally sensitized patients. Because of the easy degradability of lettuce allergens, it is recommended to patch test with freshly cut lettuce stem and supplement this with Compositae mix. As contact urticaria and protein contact dermatitis may present as dermatitis, it is important to perform prick-to-prick tests, and possibly scratch patch tests as well. Any person who is occupationally exposed to lettuce for longer periods, especially atopics, amateur gardeners, and persons keeping lettuce-eating pets, is potentially at risk of developing lettuce contact allergy. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  10. Risk factors for work-related eczema and urticaria among vocational students of agriculture.

    Science.gov (United States)

    Śpiewak, Radosław; Góra-Florek, Anna; Horoch, Andrzej; Jarosz, Mirosław J; Doryńska, Agnieszka; Golec, Marcin; Dutkiewicz, Jacek

    2017-12-23

    Farmers are at high risk of occupational skin diseases which may start already during vocational training. This study was aimed at identification of risk factors for work-related skin diseases among vocational students of agriculture. The study involved 440 students (245 males, 195 females aged 17-21 years) in 11 vocational schools which were at least 100 km from each other. The protocol included a physician-managed questionnaire and medical examination, skin prick tests, patch tests, total IgE and Phadiatop. Logistic regression model was used for the identification of relevant risk factors. Work-related dermatoses were diagnosed in 29 study participants (6.6%, 95%CI: 4.3-8.9%): eczema in 22, urticaria in 14, and co-existence of both in 7 students. Significant risk factors for work-related eczema were: history of respiratory allergy (OR=10.10; pagriculture. Atopy, past history of asthma, allergic rhinitis, and eczema (either atopic, allergic or irritant) are relevant risk factors for work-related eczema and urticaria in young farmers, along with family history of any skin disease. Positive skin prick tests seem relevant, especially in the case of urticaria. Asking simple, aimed questions during health checks while enrolling students into agricultural schools would suffice to identify students at risk for work-related eczema and urticaria, giving them the chance for selecting a safer profession, and hopefully avoiding an occupational disease in the future.

  11. Caution: Reptile pets shuttle grasshopper allergy and asthma into homes.

    Science.gov (United States)

    Jensen-Jarolim, Erika; Pali-Schöll, Isabella; Jensen, Sebastian A F; Robibaro, Bruno; Kinaciyan, Tamar

    2015-01-01

    The numbers of reptiles in homes has at least doubled in the last decade in Europe and the USA. Reptile purchases are increasingly triggered by the attempt to avoid potentially allergenic fur pets like dogs and cats. Consequently, reptiles are today regarded as surrogate pets initiating a closer relationship with the owner than ever previously observed. Reptile pets are mostly fed with insects, especially grasshoppers and/or locusts, which are sources for aggressive airborne allergens, best known from occupational insect breeder allergies. Exposure in homes thus introduces a new form of domestic allergy to grasshoppers and related insects. Accordingly, an 8-year old boy developed severe bronchial hypersensitivity and asthma within 4 months after purchase of a bearded dragon. The reptile was held in the living room and regularly fed with living grasshoppers. In the absence of a serological allergy diagnosis test, an IgE immunoblot on grasshopper extract and prick-to-prick test confirmed specific sensitization to grasshoppers. After 4 years of allergen avoidance, a single respiratory exposure was sufficient to trigger a severe asthma attack again in the patient. Based on literature review and the clinical example we conclude that reptile keeping is associated with introducing potent insect allergens into home environments. Patient interviews during diagnostic procedure should therefore by default include the question about reptile pets in homes.

  12. Territorial Governance. A Comparative Research of Local Agro-Food Systems in Mexico

    Directory of Open Access Journals (Sweden)

    Gerardo Torres-Salcido

    2018-01-01

    Full Text Available The article attempts to provide a theoretical discussion on territorial governance by presenting both the neo-institutionalist position and the De Sousa Santos’ alternative models, with a view of highlighting the dimensions that can be relevant to understanding the territorial dynamics of Local Agro-food Systems (LAFS. The paper aims to build up a system of indicators, structured in four dimensions, concerning the territorial governance of LAFS: (i multi-level coordination; (ii democratic participation and accountability; (iii cooperation among producers and other stakeholders and (iv relationships with the environment. We verify, as a hypothesis, that the typology of markets to which the identity-based products are directed plays a decisive role in the way that processes of territorial governance of LAFS are constructed. The results of an empirical research, developed in four LAFS in Mexico, are presented: prickly pear cactus in Morelos, blackberry in Michoacán, cuitlacoche (corn smut in Tlaxcala and coffee in Veracruz. Two types of territorial governance of LAFS may be distinguished: those that can be strengthened by the geographical and organisational proximity of the markets and the action of local stakeholders and governments—prickly pear cactus and cuitlacoche—versus those which are devoted to export and are conducted by large companies in which marketing networks involve certification mechanisms and a large number of institutions—coffee and blackberry.

  13. Association of Tourette syndrome and obsessive-compulsive disorder with allergic diseases in children and adolescents: a preliminary study.

    Science.gov (United States)

    Yuce, M; Guner, S N; Karabekiroglu, K; Baykal, S; Kilic, M; Sancak, R; Karabekiroglu, A

    2014-01-01

    To investigate the rate of allergic diseases including asthma, allergic rhinitis and eczema in children and adolescents diagnosed with obsessive-compulsive disorder (OCD) (n:26) and/or Tourette syndrome (TS) (n:32) [OCD plus TS, n:13] compared to control subjects (n:35) [total, n:80]. The symptoms of any allergic disease were assessed using the ISAAC questionnaire form. Allergy diagnoses were made by a pediatric allergy specialist. Skin prick tests were applied, and IgE levels and eosinophil counts were measured. While only one-fifth of the control subjects had allergic diseases, more than half of the children with TS and/or OCD had comorbid allergic diseases. Positive skin prick tests were greater in OCD patients compared to control subjects. There were no significant differences between the groups in terms of eosinophil counts or IgE levels. Among the allergic diseases, while allergic rhinitis was diagnosed at significantly higher rates in TS patients, eczema was significantly higher in OCD patients compared to control subjects. This preliminary study shows an association between allergic diseases and TS and/or OCD. The results revealing differences in associations between types of allergic disease (rhinitis or eczema) and neuropsychiatric disorder (tic disorder or OCD) need to be investigated in further studies with higher numbers of participants, and immune markers should be examined.

  14. Allergic colitis in infants related to cow's milk: clinical characteristics, pathologic changes, and immunologic findings.

    Science.gov (United States)

    Yu, Man-Chun; Tsai, Chia-Lun; Yang, Yao-Jong; Yang, Sing-San; Wang, Li-Hui; Lee, Chung-Ta; Jan, Ren-Long; Wang, Jiu-Yao

    2013-02-01

    Allergic colitis (AC) is an inflammatory condition characterized by eosinophils infiltrating the colonic wall. It can be a benign and/or severe illness among gastrointestinal diseases in infants. We report five infants who, since January 2009, in whom AC under fibrotic endoscopic examinations has been diagnosed. The criterion for histopathologic diagnosis of AC in this study was five or more eosinophils per high-power field. Patients' clinical symptoms, pathologic findings, and immunologic studies, such as specific antibodies against component of cow's milk protein, were compared with those of allergic children without AC and those of nonatopic control children. Histopathologic examinations of biopsy specimens revealed acute inflammation with characteristic eosinophilic infiltration of lamina propria (5-15 eosinophils per high-power field) in all five patients. They all had strongly positive skin prick tests against milk protein, which were not correlated with in vitro allergen-specific immunoglobulin (Ig) E levels. In contrast, there were significantly higher levels of IgE antibodies, and lower specific IgG4 and IgA levels to components and whole milk proteins in AC, as compared to control children without AC. Endoscopic biopsy specimens of intestine confirm the diagnosis of AC. However, allergen skin prick test and IgE antibody to milk protein components also provide helpful diagnostic tools for this rare disease in children. Copyright © 2012. Published by Elsevier B.V.

  15. Impact of glutathione on the allergenicity of the peach lipid transfer protein Pru p 3.

    Science.gov (United States)

    Gómez-Casado, C; Tordesillas, L; Kinkel, J; Starkl, P; Cuesta-Herranz, J; Roth-Walter F; Díaz-Perales, A; Jensen-Jarolim, E

    2015-01-01

    The allergenic potential of proteins can be altered under various physicochemical conditions. Glutathione (GSH) is a reducing agent that is used as an antioxidant in food products. We aimed to characterize the natural folding of peach proteins and test the allergenicity of reduced and natural Pru p 3, the major peach allergen. Pru p 3 was purified from peach, and its conformation was analyzed by means of circular dichroism. Using a thiol fluorescent probe, reduced proteins were detected in fresh peach. GSH-reduced Pru p 3 was tested in vitro for T-cell proliferation and in vivo using skin prick testing. GSH-reduced Pru p 3 produced variable skin prick reactions in peach-allergic patients. The proliferative response of peripheral blood mononuclear cells from allergic patients to reduced Pru p 3 tended to be less intense, whereas secretion of the cytokines IFN-γ, IL-5, and IL-10 was comparable. In a pool of sera from peach-allergic patients, reduction hardly impaired IgE-binding. Moreover, the stability of reduced Pru p 3 to gastrointestinal digestion was similar to that of the natural form. GSH can at least transiently reduce Pru p 3. We found that the effect of reduction on the allergenicity of Pru p 3 varied. Therefore, as an additive, GSH does not seem to eliminate the risk of reactions for peach-allergic patients.

  16. Exploring the temporal development of childhood IgE profiles to allergen components

    Directory of Open Access Journals (Sweden)

    Önell Annica

    2012-12-01

    Full Text Available Abstract Background Children often develop allergies that may or not persist into adulthood. Although the different allergic symptoms over time have been well documented, the underlying pattern of sensitization to various proteins and subsequent allergy development is unexplored. The aim was to study the sensitization pattern to allergen components over time from infancy to adulthood in a group of infants with heredity for allergic diseases. Methods IgE profiles were monitored in a group of 67 children from 6 months to 18 years using a microarray chip (ImmunoCAP® ISAC containing 103 allergen components derived from 47 allergen sources. The chip IgE profile was compared with clinical history, skin prick test results and diagnoses (atopic dermatitis, asthma and allergic rhinoconjunctivitis at each time point for each child. Results IgE profiles were unique for each child and showed broad agreement with the results of skin prick tests and doctors’ diagnoses. In addition, close examination of the IgE profiles often revealed early indication of subsequent allergies. IgE profiles also facilitated the examination of cross-reactivity contra co-sensitization, thereby greatly enhancing the possibility for managing patients. Conclusion This explorative description indicates that sensitization pattern to allergen components differs over time as well as among allergic individuals when examined with microarray technology.

  17. Patterns of psychosocial adaptation and allergic disorders in Korean schoolchildren.

    Science.gov (United States)

    Park, JinAh; Kim, Byoung-Ju; Kwon, Ji-Won; Song, Young Hwa; Yu, Jinho; Kim, Hyo-Bin; Lee, So-Yeon; Kim, Woo Kyung; Jee, Hye Mi; Kim, Kyung Won; Kim, Kyu-Earn; Hong, Soo-Jong; Shin, Yee-Jin

    2011-01-01

    To date, there is little evidence to support an association between symptoms of pediatric allergic disorders and psychosocial factors in the general population, particularly in Asian countries. The current study aims to investigate the relationship between psychosocial factors and symptoms of allergic disorders and to investigate the association between behavior problems and biomarkers of atopy. A cross-sectional survey of parental responses to the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire and the Korean version of the Child Behavior Checklist (CBCL) was conducted from one elementary school in Seoul, Korea. Skin prick tests for 18 major allergens were also performed. A total of 780 children with valid CBCL surveys were included in the study. Externalizing problems were significantly larger in children with asthmatic symptoms, while internalizing problems were significantly larger in children with symptoms of both asthma and allergic rhinitis. Social adaptations were significantly lower in children with symptoms of allergic rhinitis and atopic dermatitis. Boys with more positive allergens via the skin prick tests showed larger internalizing problems. While school children with allergic symptoms have been reported to have more difficulties with psychosocial adaptation, the patterns of psychosocial problems varied somewhat according to the types of atopic disorder. There was a positive relationship between atopy and behavior problems, especially in boys. Copyright © 2010 S. Karger AG, Basel.

  18. Evaluation of atopy in patients with COPD

    Directory of Open Access Journals (Sweden)

    Margarida Celia Lima Costa Neves

    2013-06-01

    Full Text Available OBJECTIVE: To determine the prevalence of atopy and to evaluate clinical, laboratory, and radiological profiles in patients with COPD. METHODS: This was a cross-sectional study involving outpatients with stable COPD (defined by the clinical history and a post-bronchodilator FEV1/FVC < 70% of the predicted value. The patients completed a questionnaire regarding clinical characteristics and atopy, after which they underwent nasal lavage cytology, skin prick testing, chest X-rays, arterial blood gas analyses, and determination of total serum IgE. RESULTS: Of the 149 subjects studied, 53 (35.6%, 49 (32.8%, and 88 (59.1% presented with nasal eosinophilia, a positive skin prick test result, and symptoms of allergic rhinitis, respectively. Correspondence analysis confirmed these findings, showing two distinct patterns of disease expression: atopy in patients with COPD that was less severe; and no evidence of atopy in those with COPD that was more severe (reduced FEV1 and hyperinflation. There was a statistically significant association between nasal eosinophilia and a positive bronchodilator response. CONCLUSIONS: Using simple and reproducible methods, we were able to show that there is a high frequency of atopy in patients with COPD. Monitoring inflammation in the upper airways can be a useful tool for evaluating respiratory diseases in the elderly and in those with concomitant asthma and COPD, a clinical entity not yet fully understood.

  19. Can gingival crevicular blood be relied upon for assessment of blood glucose level?

    Science.gov (United States)

    Dwivedi, Shivani; Verma, Sharmila J; Shah, Monali; Jain, Kapil

    2014-11-01

    Diabetes mellitus (DM) is undiagnosed in approximately half of the patients actually suffering from the disease. In addition, the prevalence of DM is more than twice as high as in patients with periodontitis when compared to periodontally healthy subjects. Thus, a high number of patients with periodontitis may have undiagnosed DM. The purpose of the present study was to evaluate whether blood oozing from a gingival crevice during routine periodontal examination can be used for determining glucose levels. Observational cross-sectional studies were carried out in 75 patients (43 males and 32 females) with chronic periodontitis who were divided into two groups: Group I and Group II, respectively. Blood oozing from the gingival crevices of anterior teeth following periodontal probing was collected with the stick of glucose self-monitoring device, and the blood glucose levels were measured. At the same time, finger-prick blood was taken for glucometric analysis and subsequent readings were recorded. The patient's blood glucose values ranged from 74 to 256 mg/dl. The comparison between gingival crevicular blood and finger-prick blood showed a very strong correlation, with a t value of 3.97 (at P value = 0.001). The data from this study has shown that GCB collected during diagnostic periodontal examination can be an excellent source of blood for glucometric analysis.

  20. Knowledge regarding postexposure prophylaxis of HIV among nurses

    Directory of Open Access Journals (Sweden)

    Dhital PS

    2017-04-01

    Full Text Available Puja Sharma Dhital,1 Sarojini Sharma,2 Pratik Poudel,3 Pankaj Raj Dhital4 1Adult Health Nursing, Nepal Polytechnic Institute, College of Nursing, 2Adult Health Nursing, BP Koirala Memorial Cancer Hospital, 3Department of Radiology, College of Medical Sciences, Bharatpur, 4Department of Agricultural Extension and Rural Sociology, Agriculture and Forestry University, Rampur, Nepal Abstract: Fifty nurses working in BP Koirala Memorial Cancer Hospital, Bharatpur, were selected by probability simple random sampling technique for determining the knowledge level about postexposure prophylaxis (PEP of HIV among nurses during 2014. A descriptive design, semistructured self-administered questionnaire was used for the study. The study showed that 48% of respondents had knowledge on the meaning of PEP, only 39.39% respondents were aware of the first aid management getting needle prick injury, 60% were aware of the best time to start PEP of HIV and 56% respondents had knowledge about the time schedule of HIV test after exposure. Although the respondents answered most of the questions correctly, they had knowledge deficit in certain areas. The respondents’ knowledge in this regard needs to be improved with time-to-time awareness program and periodic training, which ultimately helps to decrease the transmission of disease and reduces mortality and morbidity. Keywords: needle prick injury, transmission, PEP, HIV 

  1. The incidence of Common Allergens in Patients with Allergic Rhinitis Referred to Shahid Mofatteh Clinic, Yasuj, Iran

    Directory of Open Access Journals (Sweden)

    SH Nabavizadeh

    2013-12-01

    Background & Aim: Allergic diseases are one of the most common diseases which are more prevalent in certain seasons. The aim of this study was to determine the prevalence of respiratory and food allergies to common allergens in patients with allergic rhinitis. Methods: This cross-sectional study was performed on 184 patients including 120 males and 64 females referred to the Shahid Mofatteh Clinic with the respiratory allergy symptoms including rhinitis, sneezing and coughing during 2012-2013. Data collection, checklist registered, including demographic information and data associated with sensitivity of skin prick test of 21 allergens were selected. Data were analyzed using descriptive and inferential statistical. Results: A number of 153 patients (83.2% were allergic to one or more of the 21 allergens tested, whereas 31 patients (16.8 of them showed no sensitivity. The most common allergic reactions to insect allergens were 73 cases (47.7%, grass and turf 58 cases (37.9% percent, Beetle 55 cases (35.9%, wheat flour, 51 (33.3% walnuts, 37 (24.4% and cat fur 36 (5/23.5% respectively.. Conclusion: Susceptibility to insects, beetles and grass had the highest prevalence in patients with allergic rhinitis referred to Super Specialized clinic in Yasuj. Because susceptibility to allergens depends on the environment, therefore, being aware of them can lead to avoidance and control allergic reactions. Key words: Allergic Disease, Skin Prick Test, Rhinitis

  2. Citrus allergy from pollen to clinical symptoms.

    Directory of Open Access Journals (Sweden)

    Rosa Anna Iorio

    Full Text Available Allergy to citrus fruits is often associated with pollinosis and sensitization to other plants due to a phenomenon of cross-reactivity. The aims of the present study were to highlight the cross-reactivity among citrus and the major allergenic pollens/fruits, throughout clinical and molecular investigations, and to evaluate the sensitization frequency to citrus fruits in a population of children and adults with pollinosis. We found a relevant percentage of sensitisation (39% to citrus fruits in the patients recruited and in all of them the IgE-mediated mechanism has been confirmed by the positive response to the prick-to-prick test. RT-PCR experiments showed the expression of Cit s 1, Cit s 3 and a profilin isoform, already described in apple, also in Citrus clementine pollen. Data of multiple sequence alignments demonstrated that Citrus allergens shared high percentage identity values with other clinically relevant species (i.e. Triticum aestivum, Malus domestica, confirming the possible cross-allergenicity citrus/grasses and citrus/apple. Finally, a novelty of the present work has been the expression of two phospholipaseA2 isoforms (PLA2 α and β in Citrus as well as in Triticum pollens; being PLA2 able to generate pro-inflammatory factors, this enzyme could participate in the activation of the allergenic inflammatory cascade.

  3. Effects of Glossopharyngeal Insufflation in Ankylosing Spondylitis: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Nina Brodin

    2014-01-01

    Full Text Available In Ankylosing Spondylitis (AS, thoracic range of motion is often greatly limited. The objective of the study was to describe the effects of 12 weeks of Glossopharyngeal Insufflation (GI training in patients with AS. Dynamic spirometry included vital capacity, forced expiratory volume, and peak expiratory flow. Thoracic and lumbar range of motion was assessed by tragus-to-wall distance, modified Schober test, and tape measure. Disease activity, activity limitation, and health perception were assessed using the BAS-Indices, and tension in the thoracic region during GI was assessed using the Borg CR-10 scale. Adherence to training was recorded in an activity log, along with any remarks on the training. Ten patients were recruited and six male patients fulfilled the study protocol. Three patients were able to learn GI by exceeding their maximal vital capacity with 5% using GI. A significant increase in thoracic range of motion both on costae IV (P=0.04 and at the level of the xiphoid process (P=0.04 was seen. Thus, patients with AS can practice GI, it is safe if maximal exertion is avoided, and patients with some mobility in the chest can increase their lung function substantially by performing GI during 12 weeks.

  4. Hard to Believe : Produced by Ken Stone and Irene Silber, 2015, Swoop Films and Stone Soup Productions (New York, 56 minutes, unrated).

    Science.gov (United States)

    Northam, Holly Louise

    2016-06-01

    This article presents a review of Hard to Believe, a compelling documentary reporting the forced organ procurement and death of Chinese prisoners of conscience. The documentary is targeted to ignite political and public pressure to stop these practices that are thought to be motivated by financial and political gain. Narrated by journalist and author Ethan Gutmann, the documentary pricks at the collective conscience, as credible witnesses provide evidence that point to an abrogation of every ethical principle ascribed to legitimate organ procurement.

  5. 575 Photoaging Attenuates Skin Test Response to Histamine More Than Natural Aging

    OpenAIRE

    King, Monroe James; Fitzhugh, David; Lockey, Richard F.

    2012-01-01

    Background Clinical experience suggests that skin test reactivity is often decreased in photo-exposed skin versus sun-protected skin in older individuals. The current study was designed to address whether photoaging or natural aging of skin causes a greater diminution in skin test reponse. Methods Prick-puncture skin tests to histamine were performed on sun-exposed and sun-protected areas in younger (n = 61, age 20–50) and older (n = 63, age 60–87) adult volunteers who were recruited for skin...

  6. Clinical and immunological responses to occupational exposure to alpha-amylase in the baking industry.

    OpenAIRE

    Brisman, J; Belin, L

    1991-01-01

    alpha-Amylase is a starch cleaving enzyme often used in the baking industry as a flour additive. It is usually of fungal origin, produced by Aspergillus oryzae. One previous report has shown IgE antibodies and positive skin prick test against alpha-amylase in asthmatic bakers. This paper describes four alpha-amylase sensitised index cases with occupational asthma or rhinitis and the results of a cross sectional study of 20 workers from the same factory who were also exposed to alpha-amylase p...

  7. Does IFN-γ play a role on the pathogenesis of non-atopic asthma in Latin America children?

    OpenAIRE

    Figueiredo Camila Alexandrina; Rodrigues Laura Cunha; Alcantara-Neves Neuza Maria; Cooper Philip J; Amorim Leila Denise; Silva Nivea Bispo; Cruz Alvaro A; Barreto Mauricio Lima

    2012-01-01

    Abstract In this work we explore differences in blood cells and cytokine profiles in children according to atopic status and asthma (atopic or non-atopic). The study involved measurement of Th1(IFN-γ) and Th2 (IL-5 and IL-13) cytokines in Dermatophagoides pteronyssinus stimulated peripheral blood leukocytes, blood cell count, skin prick test and specific IgE against common aeroallergens. Atopic status was associated with eosinophilia and production of Th2 type cytokines. Atopic asthma was a...

  8. Risk factors for non-atopic asthma/wheeze in children and adolescents: a systematic review

    OpenAIRE

    Strina, Agostino; Barreto, Mauricio L; Cooper, Philip J; Rodrigues, Laura C

    2014-01-01

    BACKGROUND: The study of non-atopic asthma/wheeze in children separately from atopic asthma is relatively recent. Studies have focused on single risk factors and had inconsistent findings.\\ud \\ud OBJECTIVE: To review evidence on factors associated with non-atopic asthma/wheeze in children and adolescents.\\ud \\ud METHODS: A review of studies of risk factors for non-atopic asthma/wheeze which had a non-asthmatic comparison group, and assessed atopy by skin-prick test or allergen-specific IgE.\\u...

  9. Anaphylaxis due to caffeine

    OpenAIRE

    Sugiyama, Kumiya; Cho, Tatsurai; Tatewaki, Masamitsu; Onishi, Shogo; Yokoyama, Tatsuya; Yoshida, Naruo; Fujimatsu, Takayoshi; Hirata, Hirokuni; Fukuda, Takeshi; Fukushima, Yasutsugu

    2015-01-01

    We report a rare case of anaphylaxis due to caffeine intake. A 27-year-old woman suffered her first episode of anaphylaxis and a positive skin prick test suggested that the anaphylaxis was due to an IgE-mediated hypersensitivity reaction to caffeine. She was diagnosed with caffeine allergy and has not had an allergic reaction after avoiding foods and drinks containing caffeine. Although caffeine is known to have antiallergic effects, this case shows that caffeine can be an allergen and cause ...

  10. Assessment of sensitization to insect aeroallergens among patients with allergic rhinitis in Yazd City, Iran.

    OpenAIRE

    Mohammad Hassan Bemanian; Narges Alizadeh Korkinejad; Shima Shirkhoda; Mohammad Nabavi; Zahra Pourpak

    2012-01-01

    The  frequency of  allergic diseases such  as allergic rhinitis is considerable in general population. Insect aeroallergens are important allergens which can induce airway inflammation. The aim of this study was to determine the prevalence of sensitization to insect aeroallergens in allergic rhinitis patients in Yazd as a desert city in Iran.A cross-sectional study was undertaken on 95 allergic rhinitis patients who were referred to allergy clinic of Yazd city. Skin prick tests (SPT) by stand...

  11. Respiratory Effects of the Hebei Spirit Oil Spill on Children in Taean, Korea

    OpenAIRE

    Jung, Suk-Chul; Kim, Kyung-Mook; Lee, Kun-Song; Roh, Sangchul; Jeong, Woo-Chul; Kwak, Sahng-June; Lee, Ik-Jin; Choi, Young-Hyun; Noh, Su Ryeon; Hur, Jong-Il; Jee, Young-Koo

    2013-01-01

    Purpose The oil spill from the Heibei Spirit in December 2007 contaminated the Yellow Coast of South Korea. We evaluated the respiratory effects of that spill on children who lived along the Yellow Coast. Methods Of 662 children living in the area exposed to the oil spill, 436 (65.9%) were enrolled as subjects. All subjects completed a modified International Study of Asthma and Allergies in Childhood questionnaire. A health examination, including a skin prick test, pulmonary function test, an...

  12. Respiratory symptoms and sensitization in bread and cake bakers.

    Science.gov (United States)

    Smith, T A; Smith, P W

    1998-07-01

    This purpose of this study was to examine the relationship between exposure to wheat flour, soya flour and fungal amylase and the development of work-related symptoms and sensitization in bread and cake bakery employees who have regular exposure to these substances. The study populations consisted of 394 bread bakery workers and 77 cake bakery workers whose normal jobs involved the sieving, weighing and mixing of ingredients. The groups were interviewed with the aim of identifying the prevalence, nature and pattern of any work-related respiratory symptoms. They were also skin-prick tested against the common bakery sensitizing agents, i.e., wheat flour, soya flour, rice flour and fungal amylase. The results of personal sampling for sieving, weighing and mixing operations at the bakeries from which the study groups were taken were collated in order to determine typical exposures to total inhalable dust from the ingredients, expressed as 8 hour time-weighted average exposures. Data from the health surveillance and collated dust measurements were compared with the aim of establishing an exposure-response relationship for sensitization. The prevalence of work-related symptoms in bread bakery and cake bakery ingredient handlers was 20.4% and 10.4% respectively. However, in a large proportion of those reporting symptoms in connection with work, the symptoms were intermittent and of short duration. It is considered that the aetiology of such symptoms is likely to be due to a non-specific irritant effect of high total dust levels, rather than allergy. None of the cake bakers and only 3.1% of the bread bakers had symptoms which were thought to be due to allergy to baking ingredients. Using skin-prick testing as a marker of sensitization, the prevalence of positive tests to wheat flour was 6% for the bread bakers and 3% for the cake bakers. Comparable prevalences for soya flour were 7% and 1% respectively. However, the prevalence of positive skin-prick tests to fungal amylase

  13. The Hand Eczema Trial (HET): Design of a randomised clinical trial of the effect of classification and individual counselling versus no intervention among health-care workers with hand eczema

    DEFF Research Database (Denmark)

    Ibler, Kristina Sophie; Agner, Tove; Hansen, Jane L.

    2010-01-01

    . The experimental group undergoes patch and prick testing; classification of the hand eczema; demonstration of hand washing and appliance of emollients; individual counselling, and a skin-care programme. The control group receives no intervention. All participants are reassessed after six months. The primary...... strategies are needed to reduce occupational hand eczema. METHODS/DESIGN: We describe the design of a randomised clinical trial to investigate the effects of classification of hand eczema plus individual counselling versus no intervention. The trial includes health-care workers with hand eczema identified...

  14. Collecting succulent plants for deposition in a herbarium

    CSIR Research Space (South Africa)

    Walters, Michele

    2011-06-01

    Full Text Available with their unwelcoming, prickly habit may seem like more effort than it is worth. For this reason, cacti are often not collected and are thus poorly represented in herbaria (Leuenberger, 1987). This is particularly true for southern Africa where, except for one...�DUH�YDOXDEOH�FRPSOLPHQWDU\\�LGHQWL¿FDWLRQ�WRROV� that provide information on habit or other characters not always visible on dried, preserved specimens (Leuenberger, 1987). 31 Fig. 26. Collecting plant material for deposition in a herbarium. (Picture by SANBI) 2WKHU...

  15. Salivary glucose levels and its correlation with serum glucose and glycemic status in Diabetics

    Directory of Open Access Journals (Sweden)

    Vagish Kumar L S

    2014-02-01

    Conclusion:-Saliva can be used as a routine potential diagnostic tool in assessing diabetes mellitus, the most prevalent among Indian population. It is a simple and non invasive technique in screening and monitoring of this disease. Repeated painful pricks, hazard of getting infections, complications in hemophiliac patients and various other disadvantages that involve the blood tests currently used for diagnosis and monitoring of this widely prevalent Diabetes mellitus disease, can be replaced by non invasive tests involving Saliva, which is also a cost effective.

  16. In the finger it lingers

    Directory of Open Access Journals (Sweden)

    Irfan Mohamad

    2017-08-01

    Full Text Available A previously healthy 80-year-old woman presented with a history of a thorn prick injury over the distal phalange of her left finger obtained while gardening two months ago. She claimed to have a non-healing cut with a nodular lesion, which progressively increased in size, extending upwards towards the region of her left arm. There was no fever or palpable lymph nodes in the axillary region. She had been prescribed antibiotics from the local hospital but her condition did not improve.

  17. Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity

    Directory of Open Access Journals (Sweden)

    Bolhaar Suzanne THP

    2008-11-01

    Full Text Available Abstract Background Mal d 1 is a major apple allergen causing food allergic symptoms of the oral allergy syndrome (OAS in birch-pollen sensitised patients. The Mal d 1 gene family is known to have at least 7 intron-containing and 11 intronless members that have been mapped in clusters on three linkage groups. In this study, the allelic diversity of the seven intron-containing Mal d 1 genes was assessed among a set of apple cultivars by sequencing or indirectly through pedigree genotyping. Protein variant constitutions were subsequently compared with Skin Prick Test (SPT responses to study the association of deduced protein variants with allergenicity in a set of 14 cultivars. Results From the seven intron-containing Mal d 1 genes investigated, Mal d 1.01 and Mal d 1.02 were highly conserved, as nine out of ten cultivars coded for the same protein variant, while only one cultivar coded for a second variant. Mal d 1.04, Mal d 1.05 and Mal d 1.06 A, B and C were more variable, coding for three to six different protein variants. Comparison of Mal d 1 allelic composition between the high-allergenic cultivar Golden Delicious and the low-allergenic cultivars Santana and Priscilla, which are linked in pedigree, showed an association between the protein variants coded by the Mal d 1.04 and -1.06A genes (both located on linkage group 16 with allergenicity. This association was confirmed in 10 other cultivars. In addition, Mal d 1.06A allele dosage effects associated with the degree of allergenicity based on prick to prick testing. Conversely, no associations were observed for the protein variants coded by the Mal d 1.01 (on linkage group 13, -1.02, -1.06B, -1.06C genes (all on linkage group 16, nor by the Mal d 1.05 gene (on linkage group 6. Conclusion Protein variant compositions of Mal d 1.04 and -1.06A and, in case of Mal d 1.06A, allele doses are associated with the differences in allergenicity among fourteen apple cultivars. This information

  18. A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA card dried blood spots.

    Science.gov (United States)

    Medeiros, Jansen Fernandes; Almeida, Tatiana Amaral Pires; Silva, Lucyane Bastos Tavares; Rubio, Jose Miguel; Crainey, James Lee; Pessoa, Felipe Arley Costa; Luz, Sergio Luiz Bessa

    2015-05-20

    Mansonella ozzardi is a poorly understood human filarial parasite with a broad distribution throughout Latin America. Most of what is known about its parasitism has come from epidemiological studies that have estimated parasite incidence using light microscopy. Light microscopy can, however, miss lighter, submicroscopic, infections. In this study we have compared M. ozzardi incidence estimates made using light microscopy, with estimates made using PCR. 214 DNA extracts made from Large Volume Venous Blood Samples (LVVBS) were taken from volunteers from two study sites in the Rio Solimões region: Codajás [n = 109] and Tefé [n = 105] and were subsequently assayed for M. ozzardi parasitism using a diagnostic PCR (Mo-dPCR). Peripheral finger-prick blood samples were taken from the same individuals and used for microscopic examination. Finger-prick blood, taken from individuals from Tefé, was also used for the creation of FTAcard dried blood spots (DBS) that were subsequently subjected to Mo-dPCR. Overall M. ozzardi incidence estimates made with LVVBS PCRs were 1.8 times higher than those made using microscopy (44.9% [96/214] compared with 24.3% [52/214]) and 1.5 times higher than the PCR estimates made from FTAcard DBS (48/105 versus 31/105). PCR-based detection of FTAcard DBS proved 1.3 times more sensitive at diagnosing infections from peripheral blood samples than light microscopy did: detecting 24/105 compared with 31/105. PCR of LVVBS reported the fewest number of false negatives, detecting: 44 of 52 (84.6%) individuals diagnosed by microscopy; 27 of 31 (87.1%) of those diagnosed positive from DBSs and 17 out of 18 (94.4%) of those diagnosed as positive by both alternative methodologies. In this study, Mo-dPCR of LVVBS was by far the most sensitive method of detecting M. ozzardi infections and detected submicroscopic infections. Mo-dPCR FTAcard DBS also provided a more sensitive test for M. ozzardi diagnosis than light microscopy based diagnosis did and

  19. Health effects of daily airborne particle dose in children: Direct association between personal dose and respiratory health effects

    International Nuclear Information System (INIS)

    Buonanno, Giorgio; Marks, Guy B.; Morawska, Lidia

    2013-01-01

    Air pollution is a widespread health problem associated with respiratory symptoms. Continuous exposure monitoring was performed to estimate alveolar and tracheobronchial dose, measured as deposited surface area, for 103 children and to evaluate the long-term effects of exposure to airborne particles through spirometry, skin prick tests and measurement of exhaled nitric oxide (eNO). The mean daily alveolar deposited surface area dose received by children was 1.35 × 10 3 mm 2 . The lowest and highest particle number concentrations were found during sleeping and eating time. A significant negative association was found between changes in pulmonary function tests and individual dose estimates. Significant differences were found for asthmatics, children with allergic rhinitis and sensitive to allergens compared to healthy subjects for eNO. Variation is a child's activity over time appeared to have a strong impact on respiratory outcomes, which indicates that personal monitoring is vital for assessing the expected health effects of exposure to particles. -- Highlights: •Particle dose was estimated through personal monitoring on more than 100 children. •We focused on real-time daily dose of particle alveolar deposited surface area. •Spirometry, skin prick and exhaled Nitric Oxide tests were performed. •Negative link was found between changes in pulmonary functions and individual doses. •A child's lifestyle appeared to have a strong impact on health respiratory outcomes. -- The respiratory health effects of daily airborne particle dose on children through personal monitoring

  20. Detection of antigens of allergic diseases in children by radioallergosorbent test (RAST), 1

    International Nuclear Information System (INIS)

    Hirooka, Junko

    1977-01-01

    Detection of antigens mainly by RAST, measurement of immunoglobulin, and investigation of clinical history were performed on children with intractable bronchial asthma. The results were compared with those in cases of mild or moderate degree, and they were discussed. The obtained results were as follows: 1) Cases, of which the occurrence age of disease was under 2 years old, hold a majority in intractable cases, and the ratio was twice that of the control group. 2) A result of skin test was generally lower positive rate in the intractable group as compared to the control group. However, a result of prick test for buckwheat antigen in the intractable group showed higher positive rate than that in the control group. The intractable group tended to be separated into two extreme groups, one which showed positive to most of inhaled antigens in skin test, and another which showed negative to all antigens. 3) As a result of RAST, 13% of the intractable group showed positive to egg white out of food antigens, and it was three times the ratio of positive in the control group. One case showed strong positive to rice. 4) Two thirds of cases which showed positive in RAST for food antigens showed negative in prick test. 5) Total IgE in the serum of the intractable group was clearly lower in values than that of the control group. (Tsunoda, M.)

  1. Biofunctional activity of tortillas and bars enhanced with nopal. Preliminary assessment of functional effect after intake on the oxidative status in healthy volunteers.

    Science.gov (United States)

    Guevara-Arauza, Juan Carlos; Ornelas Paz, José de Jesús; Mendoza, Sergio Rosales; Guerra, Ruth Elena Soria; Paz Maldonado, Luz María Teresita; González, Diana Jaqueline Pimentel

    2011-03-03

    Prickly pear cactus stem (nopal) has been used in folk medicine and a raw material since ancient times. Stems have been proved to possess components with valuable biological activities: anti inflamatory, antioxidant, antiulcerogenic, hypoglycemic, and so forth. Nowadays, people consume foods not only to cover the nutritional requirements, they also demand for healty, natural and convenient foods that show biological activity. This study evaluated the bio-functional effects of consuming tortillas or bars (filled with prickly pear fruit jam) supplemented or not with nopal dietary fiber. The addition of nopal increased the fiber and polyphenols content in both tortillas (16.67%, 2.33 mg QE/L) and bars (13.79%, 1.99 mg QE/L). Furthermore the trolox equivalent antioxidant capacity (TEAC, 1.47 mmol/L), polyphenols (7.67 mg QE/L) and vitamin C (77.91 μmol/L) showed increased levels in volunteer's plasma after intake. Also lower levels of glucose (4.43 mmol/L), total cholesterol (4.27 mmol/L), LDL (1.96 mmol/L) and triglycerides (1.54 mmol/L) were observed in plasma after the supplementation scheme with nopal-based tortilla, while GSH:GSSG ratio in erythrocytes was higher. The results suggested that the intake of nopal-based tortillas with high content in fiber and antioxidant compounds can help to improve the overall oxidative status in healthy humans, which can reduce the risk of some chronic diseases. In addition, these products showed suitable physicochemical characteristics to be marketed.

  2. Hypnotic Effect and GC-MS Analysis of Seeds Extracts From Algerian Nopal (Opuntia ficus-indica L.

    Directory of Open Access Journals (Sweden)

    Farah Kenza BENATTIA

    2017-07-01

    Full Text Available Context: Sleep disorders are a frequent symptom that requires prior to any prescription medication, an etiological check to eliminate any organic or psychiatric pathology that is under other treatments and that could worsen under hypnotics.The phytochemical study of Nopal affirm to be an excellent source of natural oligoelements which may improve human health and nutrition Aims: The objective of this work is to extract and analyze the lipid fraction of prickly pear seeds (Opuntia ficus–indica. L, and to determine their hypnotic activity in order to achieve a primary pharmacological screening contained in these extracts (aqueous, hydro-ethanol. Methods: GC-MS analysis of seed oil extracted by soxhlet method, and the pharmacology experiment was performed on white mice from a Swiss race Balb/cby calculating the sleep time. Results: The oil of seeds is rich in fatty-acids poly-unsaturates, Main fatty acids identified were linoleic (C18:2, oleic (C18:1, and palmitic (C16:0, palmitoleic (C16:1 acids with respective contents 81.25%, 3.70%, and 15.03%. The extract hydro-ethanolic at the 500mg / kg dose has a moderate hypnotic effect Conclusion: This study proves that the food quality of prickly pear seeds is very important and its natural source of edible oil containing of the essential fatty-acids, and can be a base for a promising work to highlight the bioactive moleculeswithhypnotic effects, myorelaxant and tranquillizing.

  3. Effect of liposomes, vitamins A and E nasal spray in reducing the healing time after septoplasty with concurrent turbinate surgery in adolescents.

    Science.gov (United States)

    Ricciardiello, Filippo; Cantone, Elena; Abate, Teresa; DI Lullo, Antonella M; Oliva, Flavia; Iengo, Maurizio; DE Bernardo, Giuseppe

    2017-02-01

    This study evaluates endoscopic and quality of life (QoL) outcomes in two groups, composed by both allergic (A) and non-allergic (N-A) adolescents undergoing septoplasty with concurrent turbinate surgery by radiofrequency treated after surgery with nasal spray containing liposomes and vitamins A and E. This double-blind randomized study was carried out on 40 patients (22 males, 18 females, mean age 15.5±1.0 years) undergoing septoplasty and volumetric tissue reduction of inferior turbinates by radiofrequency for post-traumatic deformities and severe nasal obstruction. We enrolled 20 subjects with positive skin prick tests and 20 with negative skin prick tests (SPT) to aeroallergens. All subjects underwent the Visual Analogue Scale (VAS) questionnaire and the nasal endoscopy score by Lund and Kennedy (LK) at baseline before surgery (T0) and 15 days (T15) and 21 days (T21) after treatment. After surgery, the nose was not packed and the day after surgery all subjects were randomized into two groups, experimental arm group I (GI) and control arm group II (GII) and given the treatment, nasal spray containing liposomes composed of phospholipids, fatty acids, and vitamin A and E for group I and saline solution (sodium chloride 0.9%) for group II. After therapy, the VAS and LK scores were better (Pvitamins A and E nasal spray improves the QoL in the early postoperative period, both in A and N-A sub-groups.

  4. Hand dermatitis and symptoms from the fingers among Swedish dental personnel.

    Science.gov (United States)

    Lönnroth, E C; Shahnavaz, H

    1998-01-01

    Hand dermatitis among dental personnel has been an issue in recent years. Dental personnel manually handle polymer materials which are known to irritate skin, and also cause allergy. In addition, dental personnel wash their hands frequently, and use latex gloves, and are therefore at risk to develop hand dermatitis. To survey the occurrence of hand dermatitis among dental personnel, questionnaires were sent to all dental teams (dentist + chair assistant) working in the two northern Swedish counties. Referents were; researchers, teachers, and secretaries from a university and high schools, from the same geographical area. The response rate was 76% for dental teams, and 66% for referents. The results show that male dentists reported a significantly higher prevalence of hand dermatitis than male reference. In contrast to chair assistants and referents, dentists (both male and female) reported a higher extent of hand dermatitis on the left than on the right hand. There was an association between hand dermatitis among dental personnel and; age, eczema in childhood, and hay fever but, not with; sex, asthma, frequent washing of the hands, and glove use. Whitening of the fingers increased with increasing age among dental personnel. Pricking was also associated with frequent glove use. Pricking of the fingers was associated with sex, and 3.5 times more common among female dental personnel than male dentists. Numbness of the fingers, and finger pain was reported by more dentists than chair assistants and referents.

  5. Improvements in skin-testing technique.

    Science.gov (United States)

    Carr, Warner W

    2006-01-01

    Allergy skin testing is the primary modality used in the diagnosis of allergic diseases and guides development of treatment and avoidance plans. The goal of the Allergist is to skin test the appropriate population with a device and technique that minimizes pain while maximizing sensitivity and specificity. The debate involving the use of intradermal versus skin-prick testing in the diagnosis of aeroallergy has been long lasting. Past and present medical literature will be reviewed, establishing the lack of diagnostic use of intradermal testing in the setting of aeroallergy. New skin devices continue to be developed with a trend toward production of multidevices. Performance characteristics of various skin test devices will be reviewed with an emphasis on sensitivity, specificity, and variability of skin-prick testing devices. Significant statistical differences exist between all devices tested and reported in the literature. Whether these statistical differences equate to clinical differences is not known. With this review the practicing allergist should carefully evaluate multiple different devices and choose a device that suits their practice needs. In addition, allergists must ensure that technicians are sufficiently trained on the correct use of their device and should conduct continuing education to ensure that proper skin testing techniques are being used in their practice. Finally, the use of skin testing in pediatrics will be reviewed with a focus on safety. Care should be taken when skin testing infants < or = 6 months of age, especially in the setting of eczema and a family history of atopy.

  6. Skin reactions to histamine of healthy subjects after hypnotically induced emotions of sadness, anger, and happiness.

    Science.gov (United States)

    Zachariae, R; Jørgensen, M M; Egekvist, H; Bjerring, P

    2001-08-01

    The severity of symptoms in asthma and other hypersensitivity-related disorders has been associated with changes in mood but little is known about the mechanisms possibly mediating such a relationship. The purpose of this study was to examine the influence of mood on skin reactivity to histamine by comparing the effects of hypnotically induced emotions on flare and wheal reactions to cutaneous histamine prick tests. Fifteen highly hypnotically susceptible volunteers had their cutaneous reactivity to histamine measured before hypnosis at 1, 2, 3, 4, 5, 10, and 15 min after the histamine prick. These measurements were repeated under three hypnotically induced emotions of sadness, anger, and happiness presented in a counterbalanced order. Skin reactions were measured as change in histamine flare and wheal area in mm2 per minute. The increase in flare reaction in the time interval from 1 to 3 min during happiness and anger was significantly smaller than flare reactions during sadness (P<0.05). No effect of emotion was found for wheal reactions. Hypnotic susceptibility scores were associated with increased flare reactions at baseline (r=0.56; P<0.05) and during the condition of happiness (r=0.56; P<0.05). Our results agree with previous studies showing mood to be a predictor of cutaneous immediate-type hypersensitivity and histamine skin reactions. The results are also in concordance with earlier findings of an association between hypnotic susceptibility and increased reactivity to an allergen.

  7. Neonatal staphylococcal scalded skin syndrome: clinical and outbreak containment review.

    LENUS (Irish Health Repository)

    Neylon, Orla

    2012-01-31

    Staphylococcal scalded skin syndrome (SSSS) is a toxin-mediated exfoliating skin condition predominated by desquamation and blistering. Neonatal outbreaks have already been reported; however, our outbreak highlights the potential for SSSS following neonatal health promotion measures such as intra-muscular vitamin K administration and metabolic screening (heel prick) as well as effective case containment measures and the value of staff screening. Between February and June 2007, five confirmed cases of neonatal SSSS were identified in full-term neonates born in an Irish regional maternity hospital. All infants were treated successfully. Analysis of contact and environmental screening was undertaken, including family members and healthcare workers. Molecular typing on isolates was carried out. An outbreak control team (OCT) was assembled and took successful prospective steps to prevent further cases. All five Staphylococcus aureus isolates tested positive for exfoliative toxin A, of which two distinct strains were identified on pulsed-field gel electrophoresis analysis. Two cases followed staphylococcal inoculation during preventive measures such as intra-muscular vitamin K administration and metabolic screening (heel prick). None of the neonatal isolates were methicillin resistant. Of 259 hospital staff (70% of staff) screened, 30% were colonised with S. aureus, and 6% were positive for MRSA carriage. This is the first reported outbreak of neonatal SSSS in Ireland. Effective case containment measures and clinical value of OCT is demonstrated. Results of staff screening underlines the need for vigilance and compliance in hand disinfection strategies in maternity hospitals especially during neonatal screening and preventive procedures.

  8. Child's and parents' catastrophizing about pain is associated with procedural fear in children: a study in children with diabetes and their mothers.

    Science.gov (United States)

    Vervoort, T; Goubert, L; Vandenbossche, H; Van Aken, S; Matthys, D; Crombez, G

    2011-12-01

    The contribution of the child's and parents' catastrophizing about pain was explored in explaining procedural pain and fear in children. Procedural fear and pain were investigated in 44 children with Type I diabetes undergoing a finger prick. The relationships between parents' catastrophizing and parents' own fear and estimates of their child's pain were also investigated. The children and their mothers completed questionnaires prior to a routine consultation with the diabetes physician. Children completed a situation-specific measure of the Pain Catastrophizing Scale for Children (PCS-C) and provided ratings of their experienced pain and fear on a 0-10 numerical rating scale (NRS). Parents completed a situation-specific measure of the Pain Catastrophizing Scale For Parents (PCS-P) d provided estimates of their child's pain and their own experienced fear on a 0-10 NRS. Analyses indicated that higher catastrophizing by children was associated with more fear and pain during the finger prick. Scores for parents' catastrophzing about their children's pain were positively related to parents' scores for their own fear, estimates of their children's pain, and child-reported fear, but not the amount of pain reported by the child. The findings attest to the importance of assessing for and targeting child and parents' catastrophizing about pain. Addressing catastrophizing and related fears and concerns of both parents and children may be necessary to assure appropriate self-management. Further investigation of the mechanisms relating catastrophizing to deleterious outcomes is warranted.

  9. Hiv, hepatitis b and hepatitis c in garbage scavengers of karachi

    International Nuclear Information System (INIS)

    Rauf, M.U.; Saleem, M.D.; Anwar, M.O.; Ahmed, G.; Memon, M.A.

    2013-01-01

    Objectives: To determine the seroprevalence of human immunodeficiency virus, hepatitis B and C, and to study the associated risk factors in garbage scavengers of Karachi. Methods: The cross-sectional study was performed in a colony inhabited by low socioeconomic garbage-collecting people. Data was collected from April to December 2011. After informed consent and pre-test counselling, a set of questions were asked from a questionnaire, and 5cc of blood was drawn for pathological testing. SPSS 18 was used for statistical analysis. Results: A total of 141 males agreed to participate in the study with a mean age of 21.33+-9.28 years. Most of the respondents (n=67; 47.5%) were in the age group 11-20 years; 86 (61%) were single; 89 (63%) were illiterate and 127 (90%) had a monthly income less than Rs.10,000. Prevalence of hepatitis B was 18.8% (n=22), followed by 8.5% (n=10) and 0.85% (n=1) of hepatitis C and HIV respectively. Besides, 108 (77%) collected needles/syringes and 76 (54%) got pricked more than once. Only 23 (16%) wore gloves while collecting garbage. Overall, 79 (56%) had a history of sexual contact; 18 (23%) had three or more partners. Conclusion: The important factors contributing to the high prevalence of hepatitis B and C were needle prick injuries, bare-handed/bare-footed collection of garbage, poor vaccination status, improper garbage disposal system and the site of waste collection. (author)

  10. Differential skin test reactivity to pollens in pollen food allergy syndrome versus allergic rhinitis.

    Science.gov (United States)

    Ta, Von; Scott, David R; Chin, William K; Wineinger, Nathan E; Kelso, John M; White, Andrew A

    2015-01-01

    Pollen food allergy syndrome (PFAS), also called oral allergy syndrome, is a form of food allergy in which uncooked foods cause allergic symptoms generally limited to the oral mucosa. It occurs in a subset of patients with pollen allergy, although not all patients have prominent rhinitis symptoms. PFAS is related to antigenic similarity between the pollen and food allergen. The size of skin test reactions in a group of subjects with pollen sensitivity with PFAS was compared with a group of subjects who were pollen sensitive and without PFAS. Self-reported rhinitis symptoms between the two groups were compared to identify if symptom severity differed. Twenty subjects with PFAS and 20 subjects with seasonal allergic rhinitis without PFAS were enrolled in the study. All the subjects underwent standard skin-prick testing to a panel of common allergens, including select fresh fruits and vegetables. The subjects completed a Mini Rhinoconjunctivitis Quality of Life Questionnaire as part of their clinical evaluation. The subjects with PFAS and those without PFAS were compared statistically. The subjects with PFAS had significantly larger-sized skin-prick test results specific to pollens (p PFAS reported milder nasal symptoms in relation to pollen skin test result size when compared with allergic rhinitis controls without PFAS. Our study outlined basic differences between two seemingly similar patient groups with a particularly striking discordance between skin test result sizes and rhinitis symptoms. This discordance should be explored further to increase mechanistic understanding of allergen cross-reactivity in PFAS.

  11. Microgeographic factors and patterns of aeroallergen sensitisation.

    Science.gov (United States)

    Kam, Andrew W; Tong, Winnie Wy; Christensen, Jenna M; Katelaris, Constance H; Rimmer, Janet; Harvey, Richard J

    2016-10-03

    To examine patterns of airborne allergen (aeroallergen) sensitisation in the Greater Sydney area (Sydney), and their relationships with climate, coastal proximity and environment (urban v regional). Retrospective cross-sectional study of patients who underwent aeroallergen skin prick testing at three Sydney allergy clinics, January 2001 - October 2014. Proportions of patients sensitised to specific aeroallergen types; relationships between sensitisation patterns and climate and geography. Of 1421 patients who met the selection criteria (mean age, 28.3 years [SD, 21.3]; 53.3% were female), 1092 (76.8%) were sensitised to at least one aeroallergen. Those living less than 15 km from the coast were less commonly sensitised to cockroach ( 30 km, 39.7%; P  30 km, 58.1%; P applied to mould, weed and tree aeroallergens. Subtropical grass sensitisation was more common in temperate/warm summer climates (about 50%) than in temperate/hot summer (27.1%) or subtropical climates (15%) (P < 0.001), and less common in urban (36.7%) than in regional areas (54%; P = 0.014). 72.4% of grass-sensitised patients were co-sensitised to both temperate and subtropical grasses. A selected ten-aeroallergen skin prick test panel identified 98.5% of atopic patients in this Sydney sample. Environmental and geographic factors are associated with different patterns of allergic sensitisation in Sydney. Extensive co-sensitisation to subtropical and temperate grasses has implications for immunotherapy in Australia, where most currently available therapies are based on formulations directed at temperate grasses only.

  12. Time course of the effects of carbon monoxide on visual thresholds

    Energy Technology Data Exchange (ETDEWEB)

    Halperin, M H; McFarland, R A; Niven, J I; Roughton, F J.W.

    1959-01-01

    Light intensity on the visual threshold (dim background) was measured in trained volunteers during exposure to CO, O/sub 2/, low O/sub 2/, or carbogen (93% O/sub 2/--7% CO/sub 2/). CO administered in bolus doses through face mask at intervals raised COHb (finger prick samples). COHb as low as 4.5% impaired visual sensitivity. 15.8% COHb produced an impairment that was similar to that from low O/sub 2/ equivalent to 15,400 ft altitude. The visual impairment persisted in spite of gradual loss of CO from blood.

  13. Asymptomatic skin sensitization to birch predicts later development of birch pollen allergy in adults

    DEFF Research Database (Denmark)

    Bødtger, Uffe; Poulsen, Lars K; Malling, Hans-Jørgen

    2003-01-01

    = 6) were followed through use of daily diary cards during 3 consecutive birch pollen seasons. At inclusion and at the 3-year follow-up visit, conjunctival and nasal challenges, intradermal late-phase reaction evaluation, and measurement of specific IgE were performed. RESULTS: Asymptomatic sensitized...... a clinical characterization of skin test-positive subjects without symptoms and to ascertain the predictive values of common allergologic tests. METHODS: Asymptomatic adults with positive skin prick test results for birch (n = 15), nonatopic control subjects (n = 25), and birch pollen-allergic patients (n...

  14. Grass pollen symptoms interfere with the recollection of birch pollen symptoms - a prospective study of suspected, asymptomatic skin sensitization

    DEFF Research Database (Denmark)

    Assing, K; Bødtger, Uffe; Poulsen, L K

    2007-01-01

    of seasonal allergic symptoms and prospective seasonal symptom registration among subjects with AS. METHODS: On the basis of a population survey, autumn 2002, including skin prick tests (positive if > or =3 mm) and a screening questionnaire, 87 subjects with AS to birch and/or grass pollen, birch and/or grass...... days. RESULTS: Eleven AS subjects (birch: n = 10) subsequently developed allergic symptoms, yet nine admitted, at follow up, to have had symptoms before inclusion, or even denied pollen-related symptoms despite a significant diary. Compared with AS subjects sensitized to grass pollen, AS subjects...

  15. Surveillance of artemether-lumefantrine associated Plasmodium falciparum multidrug resistance protein-1 gene polymorphisms in Tanzania

    DEFF Research Database (Denmark)

    Kavishe, Reginald A; Paulo, Petro; Kaaya, Robert D

    2014-01-01

    falciparum positive dried blood spots on filter paper and rapid diagnostic test strips collected by finger pricks from patients attending health facilities in six regions of Tanzania mainland between June 2010 and August 2011 were used. Polymerase chain reaction-restriction fragment length polymorphism (PCR...... of common Pfmdr1 haplotypes reflecting strict implementation of ALu policy in Tanzania with overall prevalence of NFD haplotype ranging from 17 to 26% among other haplotypes. With continuation of ALu as first-line drug this haplotype is expected to keep rising, thus there is need for continued...

  16. The sweet Christmas rash (case series)

    DEFF Research Database (Denmark)

    Gyldenløve, Mette; Nepper-Christensen, Steen; Thyssen, Jacob P

    2013-01-01

    Christmas tree hypersensitivity is a rare condition, which has so far obtained scarce attention in the medical literature. We present two clinical cases of hypersensitivity associated with Christmas tree exposure, a 51-year-old woman with allergic contact dermatitis and a 41-year-old man...... with allergic rhinitis. The female patient had a positive patch test reaction to colophony, and the male patient had a positive skin prick test reaction to alternaria mould. Both were successfully advised to avoid prolonged exposure to Christmas trees and buy artificial trees for Christmas....

  17. [Facial and oropharyngeal angioedema in patient with alimentary fish allergy. Diagnosis and treatment].

    Science.gov (United States)

    Pino Rivero, V; Rodríguez Carmona, M; Iglesias González, R J; del Castillo Beneyto, F

    2007-01-01

    Vegetal or animal food can produce hipersensibility reactions IgE mediated of diverse intensity. We report the case of a 54 years old woman without previous allergic antecedents who after eating frozen fish had to go to Emergencies due to angioedema especially in face and oropharynx. The ENT exploration by fibroscopia descarted laryngeal edema but the patient showed initially respiratory symptoms so she was treated with SC adrenalina and then steroids during her admission. The diagnosis of alimentary alergia would be confirmed after by Allergology with cutaneous test prick type.

  18. Skin test reactivity among Danish children measured 15 years apart

    DEFF Research Database (Denmark)

    Thomsen, SF; Ulrik, Charlotte Suppli; Porsbjerg, C

    2006-01-01

    BACKGROUND: Knowledge of secular trends in the prevalence of allergy among children stems in large part from questionnaire surveys, whereas repeated cross-sectional studies using objective markers of atopic sensitization are sparse. OBJECTIVES: To investigate whether the prevalence of skin prick...... (n = 527) and the second in 2001 (n = 480). Skin test reactivity to nine common aeroallergens was measured at both occasions. RESULTS: The prevalence of positive SPT to at least one allergen decreased from 24.1% in 1986 to 18.9% in 2001, (p = 0.05). We found a declining prevalence of sensitization...

  19. Breast-feeding does not protect against allergic sensitization in early childhood and allergy-associated disease at age 7 years

    DEFF Research Database (Denmark)

    Jelding-Dannemand, Ea; Malby Schoos, Ann-Marie; Bisgaard, Hans

    2015-01-01

    clinic at 7 years of age, strictly adhering to predefined algorithms. Associations between duration of exclusive breast-feeding and outcomes were analyzed by logistic regression. RESULTS: We found no significant association between duration of exclusive breast-feeding and development of sensitization......% CI, 0.82-1.14]; and OR, 1.02 [95% CI, 0.84-1.23], respectively). Adjusting for reverse causation by excluding children with eczema, wheeze, or a positive skin prick test response before ending exclusive breast-feeding did not alter the results. CONCLUSION: Exclusive breast-feeding does not affect...

  20. Do patients with chronic rhinosinusitis benefit from consultation with an ENT-doctor?

    DEFF Research Database (Denmark)

    Lange, Bibi; Thilsing, Trine; Baelum, Jesper

    2015-01-01

    CONCLUSION: By consulting an ENT-doctor, patients with chronic rhinosinusitis (CRS), in the general population, receive disease information and adjustment of treatment which can improve disease-specific Quality-of-Life and may improve objective measurements. OBJECTIVES: This study aims to follow......, peak nasal inspiratory flow, smell test, and skin prick test. RESULTS: Out of 91 persons with CRS, only 42% had previously consulted an ENT-doctor, and 51% were in current treatment for CRS. Most patients were advised medical treatment and 20% underwent surgery. Disease-specific Quality-of-Life, peak...

  1. Seasonal variation in food allergy to apple

    DEFF Research Database (Denmark)

    Skamstrup Hansen, K; Vieths, S; Vestergaard, H

    2001-01-01

    The aim of the study was to investigate the possibility of a seasonal variation in reactivity to apples in 27 birch pollen allergic patients. Before and during the birch pollen season 1998, the patients were subjected to double-blind, placebo-controlled food challenges (DBPCFCs) with grated fresh...... Golden Delicious apple followed by an open food challenge with whole fresh apple. The clinical reactions elicited during the challenges were evaluated both by the patients and the investigators. Moreover, the skin reactivity and the in vitro reactivity to apple were evaluated by skin prick test (SPT...

  2. Point-of-care technologies for molecular diagnostics using a drop of blood.

    Science.gov (United States)

    Song, Yujun; Huang, Yu-Yen; Liu, Xuewu; Zhang, Xiaojing; Ferrari, Mauro; Qin, Lidong

    2014-03-01

    Molecular diagnostics is crucial for prevention, identification, and treatment of disease. Traditional technologies for molecular diagnostics using blood are limited to laboratory use because they rely on sample purification and sophisticated instruments, are labor and time intensive, expensive, and require highly trained operators. This review discusses the frontiers of point-of-care (POC) diagnostic technologies using a drop of blood obtained from a finger prick. These technologies, including emerging biotechnologies, nanotechnologies, and microfluidics, hold the potential for rapid, accurate, and inexpensive disease diagnostics. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. The prevalence of skin-test-positive allergic rhinitis in Danish adults

    DEFF Research Database (Denmark)

    Linneberg, A; Jørgensen, T; Nielsen, N H

    2000-01-01

    BACKGROUND: It is disputed whether increases in self-reported respiratory allergy represent a true increase or merely increased recognition. We aimed to investigate whether the prevalence of skin-prick-test (SPT)-positive allergic rhinitis had increased in an adult general population in Copenhagen...... (participation rate 74.6%) and 482 (participation rate 53.4%) subjects were examined in 1990 and 1998, respectively. Diagnoses of SPT-positive allergic rhinitis were based on a history of nasal symptoms on exposure to allergens and SPT positivity to allergens. RESULTS: The prevalence of a diagnosis of SPT...

  4. Isolation and characterization of allergens of Prosopis juliflora pollen grains.

    Science.gov (United States)

    Thakur, I S; Sharma, J D

    1985-12-01

    Proteins and glycoproteins from Prosopis juliflora (Pj) pollen grains were separated by gel filtration, electrophoresis, DEAE cellulose chromatography and their molecular weight was determined by gel filtration and SDS-Polyacrylamide gel electrophoresis. The allergenic activity of different fractions were evaluated by in vivo skin prick test and in vitro gel diffusion test. It was found that fraction E of gel filtration and fraction III and IV of DEAE cellulose chromatography were most allergenic. This fraction E of gel filtration showed positive reaction with periodic acid Schiff's reagent as determined by SDS-gel electrophoresis.

  5. Drying of prickly pear cactus cladodes (Opuntia ficus indica) in a forced convection tunnel

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, R.; de Ita, A.; Vaca, M. [Universidad Autonoma Metropolitana-Azcapotzalco, DePt. de Energia, Area de Termofluidos, Av. San Pablo 180, Col. Reynosa Tamaulipas, Del. Azcapotzalco, C.P. 02200, Mexico D.F. (Mexico)

    2009-09-15

    In this work we evaluated the kinetics of drying of Opuntia's cladodes observing two conditions: complete cladode with the protective cuticle of the intact product and with reduced cuticle (partially removed), using a drying tunnel with forced flow. The temperature of the air was set at 35, 45, and 60 C with velocities of 1.5 and 3.0 m/s. The conditions of the environment were controlled and maintained at 22 C and 30% of relative humidity. The results show that the drying time was considerably reduced when approximately 30% of the cuticle that protects the product was removed. Additionally, the temperature had greater influence than the velocity of the air. The numerical model that best describes the behavior of the drying process is the double logarithmic one, with the imposed restrictions of r close to the unit, the lowest possible {chi}{sup 2} and the RSEM tending to zero. The characteristic drying function of the product resulted in a third-grade exponential curve, where r and SD were the corresponding selection criteria. (author)

  6. Drying of prickly pear cactus cladodes (Opuntia ficus indica) in a forced convection tunnel

    International Nuclear Information System (INIS)

    Lopez, R.; Ita, A. de; Vaca, M.

    2009-01-01

    In this work we evaluated the kinetics of drying of Opuntia's cladodes observing two conditions: complete cladode with the protective cuticle of the intact product and with reduced cuticle (partially removed), using a drying tunnel with forced flow. The temperature of the air was set at 35, 45, and 60 deg. C with velocities of 1.5 and 3.0 m/s. The conditions of the environment were controlled and maintained at 22 deg. C and 30% of relative humidity. The results show that the drying time was considerably reduced when approximately 30% of the cuticle that protects the product was removed. Additionally, the temperature had greater influence than the velocity of the air. The numerical model that best describes the behavior of the drying process is the double logarithmic one, with the imposed restrictions of r close to the unit, the lowest possible χ 2 and the RSEM tending to zero. The characteristic drying function of the product resulted in a third-grade exponential curve, where r and SD were the corresponding selection criteria.

  7. [Succession pattern of artificial vegetation community and its ecological mechanism in an arid desert region].

    Science.gov (United States)

    Xu, Cailin; Li, Zizhen

    2003-09-01

    Focusing on the artificial vegetation protection system of the Shapotou section of Baotou-Lanzhou railway in the arid desert region of China, this paper examined the dynamics of dominant plant species and the succession pattern of artificial plant community in the process of establishing and developing regional artificial vegetation. It also studied the driving force and the ecologically intrinsic mechanism of the community succession. The results demonstrated that the species composition of the artificial vegetation dramatically changed after 40 years of succession, from original artificial plant community of shrub and semi-shrub to artificial-natural desert plant community with annual herb dominated. During the process of succession, the importance values of artificial shrubs, such as Caragana korshinskii and Hedysarum scoparius, decreased and gradually retreated from the artificial plant community, while the naturally multiplied annual herb, such as Eragrostis poaeoides, Bassia dasyphylla, Salsola ruthenica, Chloris virgata and etc., were presented one after another and gradually became dominant. Besides, Artemisia ordosica always played a key role in the community due to its ability of naturally sowing and self-replacement. This type of succession pattern was closely related to the shortage of precipitation resource in this region and the formation of soil crust which inhibited the reproduction of shrub and perennial herb with deep root systems. This study provided a theoretical ground for realizing persistent development of artificial plant community.

  8. Improving car passengers' comfort and experience by supporting the use of handheld devices.

    Science.gov (United States)

    van Veen, S A T; Hiemstra-van Mastrigt, S; Kamp, I; Vink, P

    2014-01-01

    There is a demand for interiors to support other activities in a car than controlling the vehicle. Currently, this is the case for the car passengers and--in the future--autonomous driving cars will also facilitate drivers to perform other activities. One of these activities is working with handheld devices. Previous research shows that people experience problems when using handheld devices in a moving vehicle and the use of handheld devices generally causes unwanted neck flexion [Young et al. 2012; Sin and Zu 2011; Gold et al.2011]. In this study, armrests are designed to support the arms when using handheld devices in a driving car in order to decrease neck flexion. Neck flexion was measured by attaching markers on the C7 and tragus. Discomfort was indicated on a body map on a scale 1-10. User experience was evaluated in a semi-structured interview. Neck flexion is significantly decreased by the support of the armrests and approaches a neutral position. Furthermore, overall comfort and comfort in the neck region specifically are significantly increased. Subjects appreciate the body posture facilitated by the armrests and 9 out of 10 prefer using handheld devices with the armrests compared to using handheld devices without the armrests. More efforts are needed to develop the mock-up into an established product, but the angles and dimensions presented in this study could serve as guidelines.

  9. Herbaceous vegetation restoration potential and soil physical condition in a mountain grazing land of Eastern Tigray, Ethiopia

    Directory of Open Access Journals (Sweden)

    Gebrewahd Amha Abesha

    2014-06-01

    Full Text Available An existence of information in the form database and full knowledge of grazing land vegetation resources and trend over time is essential for management decisions. This study was conducted in Kiltew -Awelaelo, eastern Tigray, Ethiopia. The study aimed to investigate species composition and diversity of the herbaceous vegetation, and examine the physical soil condition of the grazing lands. A total of 45 quadrats measuring 20m×20m (400m2 were laid out in 15 sample sites from three corresponding land use types (i.e. ten year enclosure, five year enclosure and open grazing land. From each land use type five sites having three quadrats were investigated. Each quadrat was laid out at an interval of 400m in five parallel transects each 200m apart from other. To collect data of herbaceous and soil five randomly located 1m2 area each, was selected and marked, within each 400m2 sample quadrat of sample sites located along the main transect. There was significant (PBracharia sp., Bromus pectinatus, Chloris gayana, Cenchurs cilarias, chloris radiata, Cynodon dactylon, Dactyloctenium aegyptium, Digitaria Velutina, Eragrostis teniufolia, Lintonia nutans, Setaria pumila, Seteria verticillate, and Tragus racemosus all occurred frequently forming the major constituents of the sites. Therefore, regeneration from area enclosure can be on advocated practice for grazing lands rehabilitation.

  10. Allergy tests do not predict food triggers in adult patients with eosinophilic oesophagitis. A comprehensive prospective study using five modalities.

    Science.gov (United States)

    Philpott, H; Nandurkar, S; Royce, S G; Thien, F; Gibson, P R

    2016-08-01

    The use of allergy tests to guide dietary treatment for eosinophilic oesophagitis (EoE) is controversial and data are limited. Aeroallergen sensitisation patterns and food triggers have been defined in Northern Hemisphere cohorts only. To determine if allergy tests that are routinely available can predict food triggers in adult patients with EoE. To define the food triggers and aeroallergen sensitisation patterns in a novel Southern Hemisphere (Australian) cohort of patients. Consecutive patients with EoE who elected to undergo dietary therapy were prospectively assessed, demographic details and atopic characteristics recorded, and allergy tests, comprising skin-prick and skin-patch tests, serum allergen-specific IgE, basophil activation test and serum food-specific IgG, were performed. Patients underwent a six-food elimination diet with a structured algorithm that included endoscopic and histological examination of the oesophagus a minimum of 2 weeks after each challenge. Response was defined as Foods defined as triggers were considered as gold standard and were compared with those identified by allergy testing. No allergy test could accurately predict actual food triggers. Concordance among skin-prick and serum allergen-specific IgE was high for aeroallergens only. Among seasonal aeroallergens, rye-grass sensitisation was predominant. Food triggers were commonly wheat, milk and egg, alone or in combination. None of the currently-available allergy tests predicts food triggers for EoE. Exclusion-rechallenge methodology with oesophageal histological assessment remains the only effective investigation. The same food triggers were identified in this southern hemisphere cohort as previously described. © 2016 John Wiley & Sons Ltd.

  11. [Frequency of rhinitis and orofacial disorders in patients with dental malocclusion].

    Science.gov (United States)

    Imbaud, Tamara Christine de Souza; Mallozi, Márcia Carvalho; Domingos, Vanda Beatriz Teixeira Coelho; Solé, Dirceu

    2016-06-01

    To describe the frequency and etiology of rhinitis, oral breathing, types of malocclusion and orofacial disorders in patients treated for dental malocclusion. Patients with poor dental occlusion (n=89, 8-15 years) undergoing orthodontic treatment at the Postgraduate Orthodontics Center (Sao Paulo, Brazil) participated in the study. Rhinitis and oral breathing were diagnosed by anamnesis, clinical assessment and allergic etiology of rhinitis through immediate hypersensitivity skin prick test (SPT) with airborne allergens. The association between types of breathing (oral or nasal), rhinitis and types of dental malocclusion, bruxism and cephalometric alterations (increased Y axis of facial growth) compared to standard cephalometric tracing (Escola de Odontologia da Universidade de São Paulo) were assessed. The frequency of rhinitis in patients with dental malocclusion was 76.4% (68), and, of these, 81.7% were allergic (49/60 positive skin prick test), whereas the frequency of oral breathing was 62.9%. There was a significant association between an increased Y axis of facial growth and oral breathing (p<0.001), as well as between oral breathing and rhinitis (p=0.009). There was no association between rhinitis and bruxism. The frequency of rhinitis in children with dental malocclusion is higher than that in the general population, which is approximately 30%. Patients with oral breathing have a tendency to a dolichofacial growth pattern (increased Y axis of facial growth). In patients with rhinitis, regardless of the presence of oral breathing, the dolichofacial growth tendency was not observed. Copyright © 2015 Sociedade de Pediatria de São Paulo. Publicado por Elsevier Editora Ltda. All rights reserved.

  12. A rare allergy to a polyether dental impression material.

    Science.gov (United States)

    Mittermüller, Pauline; Szeimies, Rolf-Markus; Landthaler, Michael; Schmalz, Gottfried

    2012-08-01

    Polyether impression materials have been used in dentistry for more than 40 years. Allergic reactions to these materials such as reported in the 1970s ceased after replacement of a catalyst. Very recently, however, patients have started to report symptoms that suggest a new allergic reaction from polyether impression materials. Here, we report on the results of allergy testing with polyether impression materials as well as with its components. Eight patients with clinical symptoms of a contact allergy (swelling, redness or blisters) after exposure to a polyether impression material were subjected to patch tests, two of them additionally to a prick test. A further patient with atypical symptoms of an allergy (nausea and vomiting after contact with a polyether impression material in the oral cavity) but with a history of other allergic reaction was also patch tested. The prick tests showed no immediate reactions in the two patients tested. In the patch tests, all eight patients with typical clinical symptoms showed positive reactions to the mixed polyether impression materials, to the base paste or to a base paste component. The patient with the atypical clinical symptoms did not show any positive patch test reactions. Polyether impression materials may evoke type IV allergic reactions. The causative agent was a component of the base paste. In consideration of the widespread use of this impression material (millions of applications per year) and in comparison to the number of adverse reactions from other dental materials, the number of such allergic reactions is very low. In very scarce cases, positive allergic reactions to polyether impression materials are possible.

  13. EVALUATION OF THE IMPACT OF THE ECKLONIA MAXIMA EXTRACT ON SELECTED MORPHOLOGICAL FEATURES OF YELLOW PINE, SPRUCE AND THUJA STABBING

    Directory of Open Access Journals (Sweden)

    Jacek Sosnowski Sosnowski

    2016-07-01

    Full Text Available The study was focused on the impact of an extract of Ecklonia maxima on selected morphological features of yellow pine (Pinus ponderosa Dougl. ex C. Lawson, prickly spruce (Picea pungens Engelm. Variety Glauca, thuja (Thuja occidentalis variety Smaragd. The experiment was established in April 12, 2012 on the forest nursery in Ceranów. April 15, 2013 was introduced research agent in the form of a spraying an aqueous solution extract of Ecklonia maxima with trade name Kelpak SL. Biologically active compounds in the extract are plant hormones: auxin and cytokinin. There were studied increment in plant height, needle length of yellow pine, twigs length in prickly spruce and thuja. The measurements of increment in length of twigs and needles were made in each case on the same, specially marked parts of plants and have carried them on the 27th of each month beginning in May and ending in September. The results were evaluated statistically using the analysis of variance. Medium differentiations were verified by Tukey's test at a significance level p ≤ 0.05. The study showed that the diversity of traits features in the experiment was depended on the extract, the tree species and the measurement time. The best results after the extract using showed a pine and spruce. Seaweed preparation contributed to increment increased of trees height for in the pine and spruce and the needles length of pine and twigs of spruce. The species showing no reaction to the extract was thuja.

  14. Surgical Elimination of the Gastric Digestion by Roux-en-Y Gastric Bypass Impacts on Food Sensitisation-a Pilot Study.

    Science.gov (United States)

    Shakeri-Leidenmühler, Soheila; Lukschal, Anna; Schultz, Cornelia; Bohdjalian, Arthur; Langer, Felix; Birsan, Tudor; Diesner, Susanne C; Greisenegger, Elli K; Scheiner, Otto; Kopp, Tamara; Jensen-Jarolim, Erika; Prager, Gerhard; Untersmayr, Eva

    2015-12-01

    Impairment of gastric digestion due to pH elevation increases the risk for food allergy induction. As patients after Roux-en-Y gastric bypass (RYGB) surgery have lower gastric acidity and less gastric gland secretion, we aimed to analyse in a prospective study the effect of limiting gastric digestion capacity by surgical intervention on the immune response towards allergens. Nine patients undergoing RYGB surgery for morbid obesity and one control patient having undergone surgery for treatment of an incisional hernia were enrolled in the study. Before and 1, 3, 6, 9 and 12 months after surgery, blood was collected for analysis of specific IgE antibodies, and patients were subjected to skin prick testing with 16 food and 18 aeroallergens. Skin prick test results revealed an increase of positive reactions indicating sensitisations towards the tested food and aeroallergens in 77.8 and 88.9 % of the patients, respectively, after surgical elimination of gastric digestion. These results were in line with elevated titers of food- and aeroallergen-specific IgE antibodies in 7 out of 9 (7/9) and 5/9 patients, respectively, after RYGB surgery. Serum cytokine levels revealed a mixed response for IFN-γ and were mostly beneath detection limit for IL-4. A change of IgE reactivity pattern occurred after impairment of gastric digestion due to surgical elimination underlining the important gastric gatekeeping function during oral sensitisation. Even though this study indicates an increased allergy risk for gastric bypass patients, further studies are needed to investigate in-depth the immunological changes associated with RYGB surgery.

  15. Blood DNA methylation biomarkers predict clinical reactivity in food-sensitized infants.

    Science.gov (United States)

    Martino, David; Dang, Thanh; Sexton-Oates, Alexandra; Prescott, Susan; Tang, Mimi L K; Dharmage, Shyamali; Gurrin, Lyle; Koplin, Jennifer; Ponsonby, Anne-Louise; Allen, Katrina J; Saffery, Richard

    2015-05-01

    The diagnosis of food allergy (FA) can be challenging because approximately half of food-sensitized patients are asymptomatic. Current diagnostic tests are excellent makers of sensitization but poor predictors of clinical reactivity. Thus oral food challenges (OFCs) are required to determine a patient's risk of reactivity. We sought to discover genomic biomarkers of clinical FA with utility for predicting food challenge outcomes. Genome-wide DNA methylation (DNAm) profiling was performed on blood mononuclear cells from volunteers who had undergone objective OFCs, concurrent skin prick tests, and specific IgE tests. Fifty-eight food-sensitized patients (aged 11-15 months) were assessed, half of whom were clinically reactive. Thirteen nonallergic control subjects were also assessed. Reproducibility was assessed in an additional 48 samples by using methylation data from an independent population of patients with clinical FA. Using a supervised learning approach, we discovered a DNAm signature of 96 CpG sites that predict clinical outcomes. Diagnostic scores were derived from these 96 methylation sites, and cutoffs were determined in a sensitivity analysis. Methylation biomarkers outperformed allergen-specific IgE and skin prick tests for predicting OFC outcomes. FA status was correctly predicted in the replication cohort with an accuracy of 79.2%. DNAm biomarkers with clinical utility for predicting food challenge outcomes are readily detectable in blood. The development of this technology in detailed follow-up studies will yield highly innovative diagnostic assays. Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  16. Feeding preference ofNezara viridula (Hemiptera: Pentatomidae and attractiveness of soybean genotypes

    Directory of Open Access Journals (Sweden)

    Efrain de Santana Souza

    2013-12-01

    Full Text Available Nezara viridula (L. (Hemiptera: Pentatomidae is a cosmopolitan insect that causes economic damages to several cultures, in particular soybeans (Glycine max [L.] Merr. Among the techniques that involve Integrated Pest Management, the resistance of plants is pointed as a tool of great value and can contribute to the reduction of populations of insects. The feeding preferences of adults of southern green stink bug (N. viridula, and the attractiveness of soybean genotypes were evaluated under laboratory conditions to detect the most resistant material against attack from this insect. A choice test, using mature grains and green pods of the genotypes was carried out, in which the number of individuals attracted in different periods was counted. Feeding preference was evaluated in the choice tests using green pods and the number of pricks and the average time spent feeding by pricks were evaluated. In addition, texture and trichome density in the green pods were evaluated. The mature grains of 'TMG 117RR' and 'TMG 121RR' were less attractive to the adults of N. viridula. Regarding the green pods, 'IAC 17' and PI 227687 were less attractive; 'IAC 17' and 'IAC PL1' were less consumed, indicating the feeding non-preference as a resistance mechanism. 'IAC 17', 'TMG-117RR' and PI 227687 presented high levels of trichome density, and in 'IAC 17' this morphological characteristic was considered to be the main resistance factor against N. viridula. These results may be useful for breeding programs that focus on the resistance of soybeans to insects.

  17. Diagnostic accuracy of patch test in children with food allergy.

    Science.gov (United States)

    Caglayan Sozmen, Sule; Povesi Dascola, Carlotta; Gioia, Edoardo; Mastrorilli, Carla; Rizzuti, Laura; Caffarelli, Carlo

    2015-08-01

    The gold standard test for confirming whether a child has clinical hypersensitivity reactions to foods is the oral food challenge. Therefore, there is increasing interest in simpler diagnostic markers of food allergy, especially in children, to avoid oral food challenge. The goal of this study was to assess the diagnostic accuracy of atopy patch test in comparison with oral food challenge. We investigated 243 children (mean age, 51 months) referred for evaluation of suspected egg or cow's milk allergy. Skin prick test and atopy patch test were carried out, and after a 2 weeks elimination diet, oral food challenge was performed. Two hundred and forty-three children underwent OFC to the suspected food. We found clinically relevant food allergies in 40 (65%) children to egg and in 22 (35%) to cow's milk. The sensitivity of skin prick test for both milk and egg was 92%, specificity 91%, positive predictive value 35%, and negative predictive value of 93%. Sensitivity, specificity, positive predictive value, and negative predictive value of atopy patch test for both milk and egg were 21%, 73%, 20%, and 74%, respectively. Our study suggests that there is insufficient evidence for the routine use of atopy patch test for the evaluation of egg and cow's milk allergy. OFC remains gold standard for the diagnosis of egg and milk allergy even in the presence of high costs in terms of both time and risks during application. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. Road map for the clinical application of the basophil activation test in food allergy.

    Science.gov (United States)

    Santos, A F; Shreffler, W G

    2017-09-01

    The diagnosis of IgE-mediated food allergy based solely on the clinical history and the documentation of specific IgE to whole allergen extract or single allergens is often ambiguous, requiring oral food challenges (OFCs), with the attendant risk and inconvenience to the patient, to confirm the diagnosis of food allergy. This is a considerable proportion of patients assessed in allergy clinics. The basophil activation test (BAT) has emerged as having superior specificity and comparable sensitivity to diagnose food allergy, when compared with skin prick test and specific IgE. BAT, therefore, may reduce the number of OFC required for accurate diagnosis, particularly positive OFC. BAT can also be used to monitor resolution of food allergy and the clinical response to immunomodulatory treatments. Given the practicalities involved in the performance of BAT, we propose that it can be applied for selected cases where the history, skin prick test and/or specific IgE are not definitive for the diagnosis of food allergy. In the cases that the BAT is positive, food allergy is sufficiently confirmed without OFC; in the cases that BAT is negative or the patient has non-responder basophils, OFC may still be indicated. However, broad clinical application of BAT demands further standardization of the laboratory procedure and of the flow cytometry data analyses, as well as clinical validation of BAT as a diagnostic test for multiple target allergens and confirmation of its feasibility and cost-effectiveness in multiple settings. © 2017 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd.

  19. Parvalbumin--the major tropical fish allergen.

    Science.gov (United States)

    Lim, Dawn Li-Chern; Neo, Keng Hwee; Yi, Fong Cheng; Chua, Kaw Yan; Goh, Denise Li-Meng; Shek, Lynette Pei-Chi; Giam, Yoke Chin; Van Bever, Hugo P S; Lee, Bee Wah

    2008-08-01

    Fish allergy is common in countries where consumption is high. Asian nations are amongst the world's largest consumers of fish but the allergen profiles of tropical fish are unknown. This study sought to evaluate the allergenicity of four commonly consumed tropical fish, the threadfin (Polynemus indicus), Indian anchovy (Stolephorus indicus), pomfret (Pampus chinensis) and tengirri (Scomberomorus guttatus). Immunoglobulin E (IgE) cross-reactivity with parvalbumin of cod fish (Gad c 1), the major fish allergen, was also studied. Detection of tropical fish and cod specific-IgE was performed by UniCap assay, and skin prick tests were also carried out. The IgE-binding components of tropical fish were identified using IgE immunoblot techniques, and cross-reactivity with Gad c 1 was assessed by ELISA inhibition and IgE immunoblot inhibition. Clinically, nine of 10 patients studied were allergic to multiple fish. All patients exhibited detectable specific-IgE to cod fish (10 of 10 skin prick test positive, eight of 10 UniCap assay positive) despite lack of previous exposure. The major allergen of the four tropical fish was the 12-kDa parvalbumin. IgE cross-reactivity of these allergens to Gad c 1 was observed to be moderate to high in the tropical fish studied. Parvalbumins are the major allergens in commonly consumed tropical fish. They are cross-reactive with each other as well as with Gad c 1. Commercial tests for cod fish appear to be sufficient for the detection of tropical fish specific-IgE.

  20. [Are amylases in bakery products and flour potential food allergens?].

    Science.gov (United States)

    Baur, X; Sander, I; Jansen, A; Czuppon, A B

    1994-05-21

    The enzyme alpha-amylase from the mould Aspergillus oryzae (Asp o II) routinely used for the production of bread, cakes and pastries has in recent years been identified as an inhalative allergen for occupational diseases (bakers' asthma). It is doubtful whether this amylase in the final product, i.e. after the baking procedure, can still be regarded as an allergen. To clarify this question, detailed case histories on 138 subjects were recorded (98 allergics, 20 patients suffering form chronic intestinal diseases, 20 healthy controls). The clinical examinations included prick skin test and IgE antibody determination using one of the customary enzyme preparations. EAST showed a few of these 138 bread consumers to be weakly sensitized to the enzyme. One of the subjects displayed a significant reaction to alpha-amylase heated to 200 degrees C. As expected, eleven bakers sensitized to alpha-amylase by inhaling it in the workplace (positive prick test, positive case history) predominantly exhibited specific IgE antibodies to the native enzyme. Apart from one weakly positive finding, heated alpha-amylase yielded negative results in this collective. Baking conditions vary widely, especially with regard to single components, temperature and duration. Thus, further investigations as to residual allergenicity or the feasible occurrence of new antigenic determinants during the production of bread, cake and pastries are required. 27% of bakers examined and 9% of atopics showed antibodies to a flour inherent enzyme, a beta-amylase. On the whole, the selected conditions hinted at a weakly sensitizing potential inherent in baking flour and in added amylase.