Sample records for saintpaulia ionantha wendl
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Micropropagation of African violet (Saintpaulia ionantha Wendl.).
Shukla, Mukund; Sullivan, J Alan; Jain, Shri Mohan; Murch, Susan J; Saxena, Praveen K
2013-01-01
Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of explants used, physiological state of the mother plant, plant growth regulators in the culture medium, and growth conditions. Development of cost-effective protocols with a high rate of multiplication is a crucial requirement for commercial application of micropropagation. The current chapter describes an optimized protocol for micropropagation of African violets using leaf explants obtained from in vitro grown plants. In this process, plant regeneration occurs via both somatic embryogenesis and shoot organogenesis simultaneously in the explants induced with the growth regulator thidiazuron (TDZ; N-phenyl-N'-1,2,3-thidiazol-5-ylurea). The protocol is simple, rapid, and efficient for large-scale propagation of African violet and the dual routes of regeneration allow for multiple applications of the technology from simple clonal propagation to induction or selection of variants to the production of synthetic seeds.
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The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl.
Directory of Open Access Journals (Sweden)
Marek Gaj
2014-01-01
Full Text Available The leaf-explants of Saintpaulia ionantha Wendl. var.'miniature' were treated by different doses of MNH and cultured on shoot regeneration medium. A strong toxic effect of some MNH doses on explant survival during the first two subcultures was noticed. The explants surviving treatment regenerated shoots with the efficiency comparable to the control. The high number of shoots regenerated from mutagenised leaves showed chlorophyll chimerism (so-called variegated forms. The use of 5 mM MNH for 1.5 or 2 h was found very effective, as 100% of survived explants regenerated variegated shoots. Besides hundreds of variegated forms also leaf-shape and flower-colour variants were observed in MNH-treated culture. Somaclonal variation was not observed in the control culture. The results indicate the great efficiency of in vitro applied MNH for in-duction of morphological variants of Saintpaulia, and especially variegated forms.
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Gray Mold on Saintpaulia ionantha Caused by Botrytis cinerea in Korea
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Hyung-Moo Kim
2011-04-01
Full Text Available Gray mold caused by Botrytis cinerea occurred on Saintpaulia ionantha in flower shop of the Jeonju city in Korea. Typical symptoms with brown water-soaked and rotting lesions were appeared on the flowers, leaves and petiole of infected plants. Many conidia spores appeared on the lesions under humid conditions. Colonies were grayish brown and sclerotial formation on potato dextrose agar. Conidia were one celled, mostly ellipsoidal or ovoid in shape, and were colorless to pale brown in color. The conidia were 7~14×5~9 μm in size. Based on pathogenicity and morphological characteristics of the isolated fungus, the causal fungus was identified as B. cinerea Persoon: Fries. Gray mold of S. ionantha was proposed to the name of this disease.
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International Nuclear Information System (INIS)
Arunee Wongpiyasatid; Peeranuch Jompuk; Katarat Chusreeaeom; Thanya Taychasinpitak
2007-01-01
Formation of adventitious plantlets on unrootedly detached leaves of two African violet (Saintpaulia ionantha) cultivars, pink and violet flowers, chronically gamma-irradiated in gamma room at The Gamma Irradiation Service and Nuclear Technology Research Center, Kasetsart University was compared. Detached leaves were immediately planted after detachment in plastic trays containing peat moss, 18 leaves per treatment with 3 replications. Three dose rates (rad/h) with 3 doses (rad)/dose rate, were applied to the irradiated samples while the controls were placed outside the gamma room. Three months after irradiation, the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf were recorded. After that, the young plantlets were transferred to the new pots for further observation on plant growth and mutation characters. The results revealed that the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf varied slightly with radiation doses but were not significantly different at different dose rates. Radiosensitivity was noticed to be higher in pink flower cultivar than the violet one. M 1 V 1 plantlets will be followed up for growth and mutation character observations
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非洲紫罗兰组织培养体系的建立%Establishing the Tissue Culture System for Saintpaulia Ionantha
Institute of Scientific and Technical Information of China (English)
尹立辉; 武术杰; 刘亚亮; 王雪松
2016-01-01
以紫色白边单瓣花的非洲紫罗兰叶片为试材进行了组织培养技术的研究。结果表明：以叶片为外植体进行组织培养时，愈伤组织诱导最适培养基为MS＋0．2 mg／L NAA＋0．5 mg／L6－BA，培养3周左右诱导率达100％；继代增殖时最适培养基为MS＋0．02 mg／L NAA＋0．1 mg／L 6－BA；无需生根培养，直接瓶外炼苗，栽植于以m （草炭土）∶m（珍珠岩）＝1∶1的基质中生长最好，成活率可达95％以上。%We studied the tissue culture technology of Saintpaulia Ionantha with the purple petals of white edge and single flower . The tissue culture was conducted with the leaves as explants .The inducing medium for callus was MS+0.2 mg/L NAA+0.5 mg/L 6-BA.The induction rate after three weeks was100 %.The most suitable medium for subculture was MS+0.02 mg/L NAA+0.1 mg/L 6-BA.The outk-tube rooting rate were relatively better without taking root in the bottle .The best matrix for planting effect was peat soil and perlite with the mixed proportion of 1∶1 and the survival rate of >95%.
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Directory of Open Access Journals (Sweden)
Ramón Zulueta-Rodríguez
2013-01-01
Full Text Available Se determinó el efecto de la inoculación de violeta africana ( Saintpaulia ionantha Wendl. con hongos micorrícico-arbusculares (HMA en un vivero comercial bajo el sistema de cultivo del productor. Se probaron dos presentaciones del inóculo, raíces frescas (IT e inoculante encapsulado en perlas de alginato de calcio (IE. Los tratamientos establecidos fueron plantas inoculadas con cada uno de los inóculos (IT e IE, plantas fertilizadas sin inocular (F, plantas inoculadas + fertilizante (IT+F e IE+F y plantas testigo (T. Las variables evaluadas 90 y 180 días después de la inoculación (DDI fueron área foliar, número de hojas, de botones florales y de flores, así como la longitud de raíz colonizada, peso seco de pecíolos, hojas y raíces al final del experimento (180 DDI. El análisis estadístico indicó diferencias altamente significativas entre los tratamientos a los 90 DDI para área foliar, número de botones florales y de flores (ANOVA, P ≤ 0.01, respuesta que prevaleció hasta los 180 DDI con respecto a las plantas testigo (ANOVA, P ≤ 0.01. En general, la interacción de los HMA con el fertilizante promovió una floración prematura, lo cual indica que el uso de estos microorganismos puede considerarse una alterna - tiva biotecnológica factible de incorporar en estos sistemas de producción.
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Broertjes, C.
1971-01-01
The differences between unfiltered X-rays from a 250/25 deep therapy apparatus and fast neutrons
The parameters used were survival of irradiated leaves of S. ionantha , production of adventitious plantlets at the base of the petiole and mutation frequency. -
Ohrui, T; Nobira, H; Sakata, Y; Taji, T; Yamamoto, C; Nishida, K; Yamakawa, T; Sasuga, Y; Yaguchi, Y; Takenaga, H; Tanaka, Shigeo
2007-12-01
The atmospheric epiphyte Tillandsia ionantha is capable of surviving drought stress for 6 months or more without any exogenous water supply via an as of yet to be determined mechanism. When plants were soaked in water for 3 h, leaves absorbed a remarkably large amount of water (30-40% on the basis of fresh weight), exhibiting a bimodal absorption pattern. Radiolabeled water was taken up by the leaves by capillary action of the epidermal trichomes within 1 min (phase 1) and then transported intracellularly to leaf tissues over 3 h (phase 2). The removal of epidermal trichome wings from leaves as well as rinsing leaves with water significantly lowered the extracellular accumulation of water on leaf surfaces. The intracellular transport of water was inhibited by mercuric chloride, implicating the involvement of a water channel aquaporin in second-phase water absorption. Four cDNA clones (TiPIP1a, TiPIP1b, TiPIP1c, and TiPIP2a) homologous to PIP family aquaporins were isolated from the leaves, and RT-PCR showed that soaking plants in water stimulated the expression of TiPIP2a mRNA, suggesting the reinforcement in ability to rapidly absorb a large amount of water. The expression of TiPIP2a complementary RNA in Xenopus oocytes enhanced permeability, and treatment with inhibitors suggested that the water channel activity of TiPIP2a protein was regulated by phosphorylation. Thus, the high water uptake capability of T. ionantha leaves surviving drought is attributable to a bimodal trichome- and aquaporin-aided water uptake system based on rapid physical collection of water and subsequent, sustained chemical absorption.
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International Nuclear Information System (INIS)
Engels, F.M.; Laan, F.M. van der; Leenhouts, H.P.; Chadwick, K.H.
1980-01-01
investigation of the nucleus of epidermal cells of the petioles of Saintpaulia leaves by cytofluorimetry revealed that all cells are in a non-cycling pre DNA synthesis phase. Cultivation of dissected leaves results in a synchronous regeneration process of a defined number of cells. Five days after onset of cultivation the cells reach the first mitosis. The nuclear development during the regeneration process is described. Irradiation of the leaves results in a directly visible inhibition of this regenerating capability which is used to quantify cell survival in a tissue. The data show that the radiation response has a similar shape to that of the survival of single cells in culture. This response can be observed before the first mitosis of the cells and its application as a new plant tissue system for cellular radiation research is discussed. (author)
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More, Shraddha K; Lande, Anirudha A; Jagdale, Priti G; Adkar, Prafulla P; Ambavade, Shirishkumar D
2013-04-01
Solanum xanthocarpum Schrad and Wendl (Kaṇṭakāri) is a diffuse herb with prickly stem, traditionally used for the treatment of inflammation and one in the group of daśamūla (group of ten herbs) herbs commonly used drug in Ayurveda. In continuation of search for potent natural anti-inflammatory agents, the present research work was planned to evaluate the anti-inflammatory activity of ethanol extract of S. xanthocarpum whole plant. The ethanol extract was evaluated at dose 10, 30 and 100 mg/kg p.o. in rats. Using pharmacological screening models carrageenan induced rat paw edema, histamine induced rat paw edema and cotton pellet granuloma in rats. Data obtained was analyzed statistically using analysis of variance followed by post-hoc Dunnett test, P < 0.05 is considered as statistically significant. Acute treatment didn't show anti-inflammatory activity against carrageenan and histamine induced paw edema. However, administration of 100 mg/kg p.o for 7 day reduced the granuloma formation in cotton pellet granuloma model. Present results support the traditional use of plant for anti-inflammatory activity. In brief, the results provide scientific pharmacological basis for the therapeutic use of S. xanthocarpum.
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Directory of Open Access Journals (Sweden)
Adriano Victor Lopes da Silva
2009-01-01
Full Text Available The objective of this work was to use a multivariate statistical method as an alternative to estimate the green biomass of the main bamboo rod, Bambusa vulgaris Schrad. i.e.: J.C. Wendl., in the presence of multicollinearity. The data came from an experiment carried out for the Agroindustrial Excelsior S. A. (Agrimex company located in the city of Goiana - PE. Quantified by their green biomass weight, 450 bamboo rods were used and 4 independent variables measured in the rod. Initially, the presence of the multicollinearity could be verified through the correlation matrix of the independent variables and the varience inflation factors, the alternative used was the regression of the principal components based on the covariate matrix. The result indicates that, when there is an interpretation to the main components, the model shows a satisfactory data adjust, and it could be used to estimate the green biomass of the main bamboo rod.
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Directory of Open Access Journals (Sweden)
Shraddha K More
2013-01-01
Full Text Available Context: Solanum xanthocarpum Schrad and Wendl (Kaṇṭakāri is a diffuse herb with prickly stem, traditionally used for the treatment of inflammation and one in the group of daśamūla (group of ten herbs herbs commonly used drug in Ayurveda. Aims: In continuation of search for potent natural anti-inflammatory agents, the present research work was planned to evaluate the anti-inflammatory activity of ethanol extract of S. xanthocarpum whole plant. Settings and Design: The ethanol extract was evaluated at dose 10, 30 and 100 mg/kg p.o. in rats. Materials and Methods: Using pharmacological screening models carrageenan induced rat paw edema, histamine induced rat paw edema and cotton pellet granuloma in rats. Statistical Analysis Used: Data obtained was analyzed statistically using analysis of variance followed by post-hoc Dunnett test, P < 0.05 is considered as statistically significant. Results: Acute treatment didn′t show anti-inflammatory activity against carrageenan and histamine induced paw edema. However, administration of 100 mg/kg p.o for 7 day reduced the granuloma formation in cotton pellet granuloma model. Conclusions: Present results support the traditional use of plant for anti-inflammatory activity. In brief, the results provide scientific pharmacological basis for the therapeutic use of S. xanthocarpum.
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Directory of Open Access Journals (Sweden)
Yuriy V. Plugatar
2018-01-01
Full Text Available Abstract. Aim of this research is to study winter resistance, drought resistance, resistance to fungal diseases and harmful insects of Washingtonia filifera (Lind. ex Andr., H. Wendl. ex A. Bary, 1879 and to examine the possibility of the subsequent selection using the experimental mutagenesis for increasing its frost resistance at the Southern Coast of the Crimea (SCC. Material and methods. The material and objects of our research were the collection plantations of Washingtonia filifera in the Arboretum of Nikitsky Botanical Garden (NBG and parks of the South Coast of Crimea. Research methods: comparative-ecological, biometric, analytical and visual. To achieve the objective, the following results were obtained in the course of the studies. The history of introduction of this species in the NBG has been described and its occurrence at the Southern Coast of the Crimea (SCC shown. An analysis of the quantitative biometric indicators of the leaves’ growth and die-back on average over the vegetation period on the basis of phenological observations over the test plants has been carried out taking into consideration the existing agricultural background. The reasons and factors influencing the frost resistance of this species depending on the combination of the set of meteorological indicators causing a certain level of frosting depending not only on the minimum negative temperatures but also on the soil and atmospheric moisture have been identified. Thresholds values of the impact of extreme negative temperatures for the Washingtonia filifera at the lethal and sub-lethal levels have been identified. Danger of infection of the representatives of the Arecaceae family (Arecaceae C.H. Schultz with the new types of dangerous phytophages has been identified. The possibility of its selection with the use of a mutagen – colchicine has been shown. Main conclusions. The criteria have been outlined which will give the opportunity in the future to provide
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Directory of Open Access Journals (Sweden)
Raul Meneses R
2012-12-01
Full Text Available Acacia saligna (Labill. H.L. Wendl. forage is an alternative feed supply for goats during dry periods It was used as feed during pregnancy and lactation to evaluate production response and some blood parameters. Six animals in each group were fed with 0, 25, 50, 75, and 100% of acacia as alfalfa (Medicago sativa L. hay replacement in a completely randomized design. Forage chemical analysis was done to calculate nutrient intake. Blood samples were analyzed for albumin, urea N, globulin, total protein, Ca, and P. Productive parameters were analyzed by ANOVA, Duncan, and regression analyses between acacia and dry matter (DM, crude protein (CP, metabolizable energy (ME, and milk production. Acacia consumption during pregnancy was 65.5% of control, affected by the consumption of CP, ME intake and body condition (P La Acacia saligna es una alternativa de alimentación para caprinos, por lo que se ofreció a hembras en prenez y lactancia para evaluar su respuesta y algunos parámetros sanguíneos. Los animales fueron asignados a grupos que recibieron 0, 25, 50, 75 y 100% de acacia en reemplazo de heno de alfalfa (Medicago sativa L., en un diseno completamente al azar. Análisis químico de forraje fue realizado para calcular consumo de nutrientes. Albumina, N úrico, globulinas, proteína total, Ca, y P fueron analizados en sangre. Se controló peso, condición corporal, y peso de nacimiento. Se realizó ANDEVA, Duncan, y regresión para acacia y las variables evaluadas. El consumo de acacia en la prenez fue 65,5% del control, afectó el consumo de proteína cruda (PC, energía metabolizable (EM y condición corporal (P < 0,01. El peso corporal no fue afectado (P < 0,01, siendo 25,9% el nivel límite de inclusión de acacia. El peso de nacimiento fue diferente con 100% de acacia (P < 0,05. En lactancia, el consumo de MS, PC, y EM aumentó (P < 0,01. Niveles de 50 y 25% acacia disminuyeron el peso y la condición corporal. El N úrico y albumina fueron
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Reverchon, Sylvie; Rouanet, Carine; Expert, Dominique; Nasser, William
2002-02-01
In the plant-pathogenic bacterium Erwinia chrysanthemi production of pectate lyases, the main virulence determinant, is modulated by a complex network involving several regulatory proteins. One of these regulators, PecS, also controls the synthesis of a blue pigment identified as indigoidine. Since production of this pigment is cryptic in the wild-type strain, E. chrysanthemi ind mutants deficient in indigoidine synthesis were isolated by screening a library of Tn5-B21 insertions in a pecS mutant. These ind mutations were localized close to the regulatory pecS-pecM locus, immediately downstream of pecM. Sequence analysis of this DNA region revealed three open reading frames, indA, indB, and indC, involved in indigoidine biosynthesis. No specific function could be assigned to IndA. In contrast, IndB displays similarity to various phosphatases involved in antibiotic synthesis and IndC reveals significant homology with many nonribosomal peptide synthetases (NRPS). The IndC product contains an adenylation domain showing the signature sequence DAWCFGLI for glutamine recognition and an oxidation domain similar to that found in various thiazole-forming NRPS. These data suggest that glutamine is the precursor of indigoidine. We assume that indigoidine results from the condensation of two glutamine molecules that have been previously cyclized by intramolecular amide bond formation and then dehydrogenated. Expression of ind genes is strongly derepressed in the pecS background, indicating that PecS is the main regulator of this secondary metabolite synthesis. DNA band shift assays support a model whereby the PecS protein represses indA and indC expression by binding to indA and indC promoter regions. The regulatory link, via pecS, between indigoidine and virulence factor production led us to explore a potential role of indigoidine in E. chrysanthemi pathogenicity. Mutants impaired in indigoidine production were unable to cause systemic invasion of potted Saintpaulia ionantha
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International Nuclear Information System (INIS)
Zhou, L.B.; Li, W.J.; Ma, S.; Dong, X.C.; Yu, L.X.; Li, Q.; Zhou, G.M.; Gao, Q.X.
2006-01-01
The effects of 960 MeV carbon ion beam and 8 MeV X-ray irradiation on adventitious shoots from in vitro leaf explants of two different Saintpaulia ionahta (Mauve and Indikon) cultivars were studied with regard to tissue increase, shoots differentiation and morphology changes in the shoots. The experimental results showed that the survival fraction of shoot formation for the Mauve and Indikon irradiated with the carbon ion beam at 20 Gy were 0.715 and 0.600, respectively, while those for both the cultivars exposed to the X-ray irradiation at the same dose were 1.000. Relative biological effectiveness (RBE) of Mauve with respect to X-ray was about two. Secondly, the percentage of regenerating explants with malformed shoots in all Mauve regenerating explants irradiated with carbon ion beam at 20 Gy accounted for 49.6%, while that irradiated with the same dose of X-ray irradiation was only 4.7%; as for Saintpaulia ionahta Indikon irradiated with 20 Gy carbon ion beam, the percentage was 43.3%, which was higher than that of X-ray irradiation. Last, many chlorophyll deficient and other varieties of mutants were obtained in this study. Based on the results above, it can be concluded that the effect of mutation induction by carbon ion beam irradiation on the leaf explants of Saintpaulia ionahta is better than that by X-ray irradiation; and the optimal mutagenic dose varies from 20 Gy to 25 Gy for carbon ion beam irradiation
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Soft rot erwiniae: from genes to genomes.
Toth, Ian K; Bell, Kenneth S; Holeva, Maria C; Birch, Paul R J
2003-01-01
SUMMARY The soft rot erwiniae, Erwinia carotovora ssp. atroseptica (Eca), E. carotovora ssp. carotovora (Ecc) and E. chrysanthemi (Ech) are major bacterial pathogens of potato and other crops world-wide. We currently understand much about how these bacteria attack plants and protect themselves against plant defences. However, the processes underlying the establishment of infection, differences in host range and their ability to survive when not causing disease, largely remain a mystery. This review will focus on our current knowledge of pathogenesis in these organisms and discuss how modern genomic approaches, including complete genome sequencing of Eca and Ech, may open the door to a new understanding of the potential subtlety and complexity of soft rot erwiniae and their interactions with plants. The soft rot erwiniae are members of the Enterobacteriaceae, along with other plant pathogens such as Erwinia amylovora and human pathogens such as Escherichia coli, Salmonella spp. and Yersinia spp. Although the genus name Erwinia is most often used to describe the group, an alternative genus name Pectobacterium was recently proposed for the soft rot species. Ech mainly affects crops and other plants in tropical and subtropical regions and has a wide host range that includes potato and the important model host African violet (Saintpaulia ionantha). Ecc affects crops and other plants in subtropical and temperate regions and has probably the widest host range, which also includes potato. Eca, on the other hand, has a host range limited almost exclusively to potato in temperate regions only. Disease symptoms: Soft rot erwiniae cause general tissue maceration, termed soft rot disease, through the production of plant cell wall degrading enzymes. Environmental factors such as temperature, low oxygen concentration and free water play an essential role in disease development. On potato, and possibly other plants, disease symptoms may differ, e.g. blackleg disease is associated
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African Journal of Biotechnology - Vol 13, No 33 (2014)
African Journals Online (AJOL)
CO2 emissions from soil incubated with sugarcane straw and nitrogen fertilizer · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. RF de Almeida, CH Silveira, JER Mikhael, FO Franco, BT Ribeiro, A de Siqueira Ferreira, E de Sa Mendonca, B Wendling ...
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International Nuclear Information System (INIS)
Chadwick, K.H.; Leenhouts, H.P.; Werry, P.A.Th.J.; Ramulu, K.S.
1980-01-01
Research was carried out on four topics: generation of cell reproductive death and mutation induction in cell cultures of Haplopappus gracilis by ionizing radiation; radiation induced repair in Saintpaulia epidermis cells; analysis of the combined effect of radiation with other mutagens; and the effect of very low doses of radiation on the Oenothera model system. (Auth.)
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Propagaçăo vegetativa de pupunheira (Bactris gasipaes H.B.K.) a partir de perfilhos /
Tracz, Anderson Luiz Augusto
2005-01-01
Orientador: Marguerite Quoirin Co-orientador: Ivar Wendling Dissertaçăo (mestrado) - Universidade Federal do Paraná, Setor de Cięncias Agrárias, Programa de Pós-Graduaçăo em Agronomia. Defesa: Curitiba, 2005 Inclui bibliografia e anexos Área de concentraçăo: Produçăo vegetal
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2006-11-01
ionantha T E Cypress domes White-flowered wild petunia Ruellia noctiflora E Wet prairie BIRDS American oystercatcher...The parking and street lights will be full cut fixtures in addition to being fully s hielded and using low pressure sodium lamps .. 2 ) The height...uction Ms .. Patrick, Thank you for the rapid response to our letter. Below are tbe r .esponses to your comments: 1 . The light wi l l be ful l cut
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Agar alternatives for micropropagation of African violet ( Saintpaulia ...
African Journals Online (AJOL)
Agar is one of the most popular solidifying agents in plant tissue culture. High price of pure grade agar and fear of over exploitation of its resources caused searching for low cost alternatives. In this study, liquid medium with cotton substratum and different combinations of starch, semolina, potato powder and agar in two ...
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Application of atomic energy in agriculture. Annual report, 1975
Energy Technology Data Exchange (ETDEWEB)
1976-01-01
Activities over the year 1975 are reviewed in the annual report for the entire research programme comprising: primary radiation effects in inert and biological material, dose fractionation effect in Saintpaulia, adventitious bud technique in mutation breeding, mutation research in potatoes, protein improvements in peas and maize, disease resistance in tomatoes, pepper, wheat and barley, food preservation, radiation genetics of insect pests, genetics of higher plants, soil-plant studies, element behaviour in the soil and water environment, heavy metals in plants and soils, development of methods and instruments.
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Application of atomic energy in agriculture (Annual Report 1974)
International Nuclear Information System (INIS)
1975-01-01
Activities and results over the year 1974 are reviewed in the annual report for the entire research programme comprising: lyoluminescence, perspex dosemeters, primary radiation effects in biological materials, dose fractionation effect in Saintpaulia, adventitious bud technique in mutation breeding, mutation research in potatoes, protein improvement in peas, disease resistance in tomatoes, wheat, peas and barley, food preservation, genetic pest control, genetics of higher plants, soil-plant studies, element behaviour in soils and groundwater, heavy metals in plants, liquid waste reuse after irradiation, development of methods and instruments
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Application of atomic energy in agriculture, annual report 1975
International Nuclear Information System (INIS)
1976-01-01
Activities over the year 1975 are reviewed in the annual report for the entire research programme comprising: primary radiation effects in inert and biological material, dose fractionation effect in Saintpaulia, adventitious bud technique in mutation breeding, mutation research in potatoes, protein improvements in peas and maize, disease resistance in tomatoes, pepper, wheat and barley, food preservation, radiation genetics of insect pests, genetics of higher plants, soil-plant studies, element behaviour in the soil and water environment, heavy metals in plants and soils, development of methods and instruments
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International Nuclear Information System (INIS)
Chong, M.S.; Nesic, D.; Kuhlmann, L.; Postoyan, R.; Varsavsky, A.; Cook, M.
2010-01-01
Full text: Epilepsy is a common neurological disease that affects 0.5-1 % of the world's population. In cases where known treatments cannot achieve complete recovery, seizure prediction is essential so that preventive measures can be undertaken to prevent resultant injury. The elcctroencephalogram (EEG) is a widely used diagnostic tool for epilepsy. However, the EEG does not provide a detailed view of the underlying seizure causing neuronal mechanisms. Knowing the dynamics of the neuronal population is useful because tracking the evolution of the neuronal mechanisms will allow us to track the brain's progression from interictal to ictal state. Wendling and colleagues proposed a parameterised mathematical model that represents the activity of interconnected neuronal populations. By modifying the parameters, this model is able to reproduce signals that are very similar to the real EEG depicting commonly observed patterns during interictal and ictal periods. The transition from non-seizure to seizure activity, as seen in the EEG. is hypothesised to be due to the impairment of inhibition. Using Wendling's model, we designed a deterministic nonlinear estimator to recover the average membrane potential of the neuronal populations from a single channel EEG signal. for any fixed and known parameter values. Our nonlinear estimator is analytically proven to asymptotically converge to the true state of the model and illustrated in simulations. We were able to computationally observe the dynamics of the three neuronal populations described in the model: excitatory, fast and slow inhibitory populations. This forms a first step towards the prediction of epileptic seiwres. (author)
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Establishment of callus from Opuntia robusta Wendl., a wild and ...
African Journals Online (AJOL)
coco santos
2013-04-22
Apr 22, 2013 ... plants produce tender cladodes, consumed as vegetable and fruits (prickly pear) .... The highest callus amount was obtained in media supplemented ... CO2 during carboxylation and decarboxylation reactions, improves the ...
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Directory of Open Access Journals (Sweden)
Maria Isabel Vallilo
2004-10-01
Full Text Available Sementes de Archontophoenix alexandrae extraídas de frutos coletados no Parque Estadual da Cantareira, São Paulo, foram analisadas quanto à composição química, ao perfil de ácidos graxos do óleo e aos teores de minerais, visando avaliar o seu potencial alimentício. Detectaram-se alto teor de fibras alimentares (38,80% p/p e umidade (47,72% p/p. Na fração oleosa, apesar do baixo conteúdo de óleo encontrado (2,74% p/p, predominaram os ácidos palmítico (19,80% p/p, entre os saturados, e oléico (42% p/p e linoléico (13% p/p, quanto aos insaturados. A presença, no óleo, de alfa-tocoferol (vitamina E equivalente a 4,0 mg 100 g-1 e de delta-tocoferol (1,8 mg 100 g-1 confere ao óleo certa estabilidade oxidativa. Embora contendo minerais como K, P, S, Ca, Fe, Zn, Se e Cu, lipídios e fibras alimentares, a presença do elemento Pb (2,74 mg kg-1 inviabiliza o consumo dessas sementes como alimento da avifauna e sinaliza contaminação antrópica no local de coleta.Seeds of Archontophoenix alexandrae, extracted from fruits collected at the Cantareira State Park, São Paulo, Brazil, were analyzed for chemical composition, oil fatty acids profiles and mineral content aiming to evaluate their nutritional potential. High values of dietary fibers (38.80% w/w and humidity (47.72% w/w were found. Palmitic acid (19.80% w/w was predominant among the saturated oil fraction, and oleic (42% w/w and linoleic (13% w/w among the insaturated oil fraction. The presence of alpha-tocopherol (vitamin E equivalent to 4.0mg 100g-1 and delta-tocopherol (1.8 mg 100g-1 confers some oxidative stability to the oil. Even containing minerals such as K, P, S, Ca, Zn, Se and Cu, lipids and dietary fibers, the presence of Pb (2.74mg kg-1 makes its consume unviable for avifauna, as well as it indicates anthropic contamination at the collect spot.
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Desiccation effects on germination and vigor of King palm seeds
Directory of Open Access Journals (Sweden)
Martins Cibele C.
2003-01-01
Full Text Available The desiccation tolerance of Archontophoenix alexandrae (Wendl. & Drude seeds was determined and the most sensitive vigor test for assessing seed deterioration of this species was identified. Mature fruits were harvested in the palm collection of the Instituto Agronomico in Campinas, Brazil. Depulped fruits were transported in impermeable packages to the Faculdade de Agronomia in Botucatu, where the seeds were dried. As the seed moisture decreased, germination, seedling length, electrical conductivity and moisture were measured. The seeds of A. alexandrae are recalcitrant, with high germination percentage (over 67% when undried (47% seed moisture. Lowering seed moisture below 31.5% reduced the germination rate significantly (<52.5%. Total germination failure was observed when seed moisture reached 15.1%. The electrical conductivity was the most sensitive vigor test to identify seed deterioration.
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Directory of Open Access Journals (Sweden)
Raúl Meneses Rojas
2004-04-01
Full Text Available Catorce procedencias de Acacia saligna fueron evaluadas en función del crecimiento a los 15 meses en dos sitios del norte de Chile. Correlaciones genéticas tipo B fueron estimadas para examinar la interacción genotipo x ambiente (G x A del crecimiento en altura total, diámetro medio de copa y diámetro de cuello. Máxima Verosimilitud Restringida (REML, fue el método utilizado para la estimación de componentes de varianza. Para el crecimiento en altura, la correlación genética fue moderada (pB = 0,46. Para diámetro de cuello y diámetro medio de copa, los valores de correlación genética mostraron un valor bajo (pB = 0,27 y 0,17, respectivamente, indicando una alta interacción G x A. Los resultados mostraron diferencias significativas entre las procedencias, en cada sitio estudiado (p ≤ 0,01. El crecimiento promedio de las procedencias varió entre los distintos sitios, mostrando mayor potencial hacia el sur de la Región. De persistir una alta interacción GxA en el tiempo, se hace necesario seleccionar grupos de procedencias específicos para cada sitio, debido a que no siempre el mejor grupo para un sitio específico mantuvo su superioridad en el otro.
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The Allelopathic Effect of the Exotic Tree Acacia saligna on the Germination of Wheat and Canola
Directory of Open Access Journals (Sweden)
Mohamed Kamel
2015-06-01
Full Text Available This study was carried out to investigate the allelopathic effect of aqueous extracts derived from leaves and stems of Acacia saligna (Labill. H.L.Wendl. upon two agricultural crops, wheat and canola. Seed germination (%, shoot and root elongation, fresh and dry weight, vigor index and phytotoxicity parameters were estimated. Leaf extract exhibits higher inhibitory effect than stem extract. Wheat seeds were more tolerant to the allelopathic action of A. saligna extracts than canola. Canola germination minimized to 8.33% at concentration 10% of leaf extract but the percent of germination was 60% in the case of stem extract. At 10% leaf extract, 76.67% of wheat seeds germinated; but at 10% stem extract, 93.33% of the seeds were germinated. The other growth parameters as shoot and root length, fresh and dry weight and vigor index also showed continued decrease with the increasing of allelopathic extract concentration. Leaf extract exhibits the stronger allelopathic effect. The phytotoxic effect was stronger on the germination of canola compared with wheat. It reached up to 91.76% inhibition at concentration 10%, but reached up only 23.33% in the case of wheat, respectively
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Directory of Open Access Journals (Sweden)
Freddy Mora Pobrete
2010-08-01
Full Text Available Catorce procedencias de Acacia saligna fueron evaluadas en función del crecimiento a los 15 meses en dos sitios del norte de Chile. Correlaciones genéticas tipo B fueron estimadas para examinar la interacción genotipo x ambiente (G x A del crecimiento en altura total, diámetro medio de copa y diámetro de cuello. Máxima Verosimilitud Restringida (REML, fue el método utilizado para la estimación de componentes de varianza. Para el crecimiento en altura, la correlación genética fue moderada (ΓpB = 0,46. Para diámetro de cuello y diámetro medio de copa, los valores de correlación genética mostraron un valor bajo (ΓpB = 0,27 y 0,17, respectivamente, indicando una alta interacción G x A. Los resultados mostraron diferencias significativas entre las procedencias, en cada sitio estudiado (p ≤ 0,01. El crecimiento promedio de las procedencias varió entre los distintos sitios, mostrando mayor potencial hacia el sur de la Región. De persistir una alta interacción GxA en el tiempo, se hace necesario seleccionar grupos de procedencias específicos para cada sitio, debido a que no siempre el mejor grupo para un sitio específico mantuvo su superioridad en el otro.
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Directory of Open Access Journals (Sweden)
M. Solgi
2015-12-01
Full Text Available One of the major advantages of in vitro culture of African violet (Saintpaulha ionantha is production of new cultivars and propagation of their chimera which might not be propagated by the other methods. In this study, we tested the effects of silver nanoparticles on the sterilization rate (antifungal and antibacterial activity, regeneration and shoot formation of African violet "Pink Amiss" explants. These nanoparticles were synthesized from pomegranate peels and Damask rose petals extracts. We used a completely randomized design test with factorial arrangement to investigate various volumetric ratios of plant extracts to silver nitrate (1:20, 1:10, 1:5 and 1:1 on the culture contaminations. Using silver nanoparticles synthesized by the plant extracts, especially Damask rose petals extract resulted in no fungal and bacterial contamination in the African violet explants after 1 and 3 weeks as compared to the control, and silver nitrate (1mM. All tested concentrations of the silver nanoparticles significantly (P &le 0.05 controlled both bacterial and fungal contaminations. The 1:20 ratio of plant extracts to silver nitrate showed the best control. In addition, the highest regeneration (%52 and shoot regeneration (%38 was observed in this treatment. In conclusion, we suggest using silver nanoparticles synthesized by plant extracts for sterilization of in Vitro Culture for African Violet rather than using other chemicals such as silver nitrate.
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A case of acute lymphoblastic leukemia with an intracerebellar mass
International Nuclear Information System (INIS)
Oshima, Yukio; Shitara, Toshiji; Kuribayashi, Toshio; Noji, Takashi; Kuroume, Takayoshi
1983-01-01
A 3-year-old boy, who had a complaint of hemorrhagic diathesis, was diagnosed as having acute lymphoblastic leukemia. Remission was induced by a combination of vincristine and prednisolone. Prophylactic intrathecal methotrexate and cranial irradiation were administered. Two years later, he was hospitalized for CNS leukemia and treated with multiple doses of intrathecal methotrexate. At the time, the results of CT scanning were normal. Six months later, though, he developed vomiting and lethargy. CT scanning showed a mass of an increased density in the right cerebellar hemisphere that displaced the fourth ventricle to the left and resulted in an obstructive hydrocephalus. Decompression was done by means of Ommaya reservoir setting. Soon his consciousness returned to normal, and CT scanning revealed no abnormal mass three weeks later. A month later, though, the CNS leukemia returned. He developed vomiting and a headache, and CT scanning showed a high-density mass in the right cerebellar hemisphere. The mass was diagnosed as hematoma. He died one month later. In this case, the previous mass showed evidence of a relatively uniform contrast enhancement, which is consistent with the intracerebral leukemic mass reported by Wendling. In Japan, this is the first report of an intracerebellar mass of acute lymphoblastic leukemia as perceived by CT scanning. (author)
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[Perioperative adverse respiratory events in overweight and obese children].
Ulrici, J; Hempel, G; Sasse, M; Vollrath, J; Höhne, C
2016-12-01
Overweight and obesity are increasing problems in pediatric anesthesia. This observational study was designed to examine how airway-related complications occur in overweight children and adolescents during general anesthesia and if this is a relevant problem in Germany. From October 2008 until August 2009, at the university clinic in Leipzig, 504 in- and outpatients, aged 2-18 years, ASA I-III, undergoing elective procedures (ENT and pediatric surgery), were observed. With the aid of special data sheets, the following parameters were determined: Mallampati Score, difficult mask ventilation and intubation, use of a Guedel/Wendl tube, Cormack-Lehane Score, number of intubation attempts, airway obstructions (broncho- and laryngospasms), coughing as a sign of airway irritation, and decreases in oxygen saturation >10 %. Overweight and obese children had a significantly higher Mallampati Score and a significantly higher prevalence of coughing (p overweight children and 6.3 % in children of normal weight, and that of airway obstructions was 4.1 vs 2.7 %. This study demonstrated a very low incidence of respiratory problems, which may be caused by the low proportion of morbidly obese children and the older age of overweight children in comparison with other studies.
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Edgardo Díaz-Alvarez
2015-04-01
Full Text Available Acoelorraphe wrightii H. Wendl is a palm native to Central America, the Caribbean and South Florida. In Cuba it is used in rural carpentry, primarily as fence posts, which could affect the dynamics of their populations. Because of this, in this work the dynamics of a population of A. wrightii and the potential impact of their use is characterized. They were identified and quantified in five transects 50 x 5m all colonies as genetic units (genets and each rod (ramets, identifying those in reproductive status . The estimated population size and life tables constructed time -specific. The population density and size are high,with an estimated more than three million ramets . However, only two top ramets to 3.60 m were found, but the average size was 2.52 m, although the species was described with a maximum of 5 m in similar conditions.Life tables of mortality showed two peaks, one between seedlings and other adults between 1.8 and 3 m in height, height they begin to be used. Also, a significant relationship between the height of individuals and their reproductive capacity is found. The cuts suffered by ramets seem to be affecting the reproductive capacity of the population. Despite the large population size, the overexploitation of reproductive individuals highlights the need to develop stra -tegies for sustainable use
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Antúnez-Lamas, María; Cabrera-Ordóñez, Ezequiel; López-Solanilla, Emilia; Raposo, Rosa; Trelles-Salazar, Oswaldo; Rodríguez-Moreno, Andrés; Rodríguez-Palenzuela, Pablo
2009-02-01
Dickeya dadantii 3937 (ex Erwinia chrysanthemi), a member of the Enterobacteriaceae, causes soft rot in many economically important crops. A successful pathogen has to reach the interior of the plant in order to cause disease. To study the role of motility and chemotaxis in the pathogenicity of D. dadantii 3937, genes involved in the chemotactic signal transduction system (cheW, cheB, cheY and cheZ) and in the structure of the flagellar motor (motA) were mutagenized. All the mutant strains grew like the wild-type in culture media, and the production and secretion of pectolytic enzymes was not affected. As expected, the swimming ability of the mutant strains was reduced with respect to the wild-type: motA (94%), cheY (80%), cheW (74%), cheB (54%) and cheZ (48%). The virulence of the mutant strains was analysed in chicory, Saintpaulia and potato. The mutant strains were also tested for their capability to enter into Arabidopsis leaves. All the mutants showed a significant decrease of virulence in certain hosts; however, the degree of virulence reduction varied depending on the virulence assay. The ability to penetrate Arabidopsis leaves was impaired in all the mutants, whereas the capacity to colonize potato tubers after artificial inoculation was affected in only two mutant strains. In general, the virulence of the mutants could be ranked as motA
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Okwu, D E; Nnamdi, Fred U
2008-01-22
The phytochemical contents and medicinal values of Dacroydes edulis and Raphia hookeri exudates were investigated. Phytochemical screening of the plant showed that they contain the presence of bioactive compounds comprising saponins (2.08-3.98 mg 100 g(-1)), alkaloids (0.28-0.49 mg 100 g(-1)), tannins (0.47-0.72 mg 100 g(-1)), flavonoids (0.26-0.39 mg 100 g(-1)), and phenolic compounds (0.01-0.05 mg 100 g(-1)). The carbohydrates, lipids and protein content were 77.42-78.90%, 2.02-4.185% and 16.63-18.38% respectively. The exudates are a good source of water soluble vitamins; ascorbic acid (7.04-26.40 mg 100 g(-1)), niacin (3.12-4.00 mg 100 g(-1)), riboflavin (0.14-0.54 mg 100 g(-1)) and thiamine (0.15-0.22 mg 100 g(-1)). Both plants exudates are good sources of minerals such as Ca, Mg, P, Fe, Zn, Cu and Mn while Cr and Co were trace. These results indicate that exudates can be potential sources of feedstock for the pharmaceutical industry.
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Zaitlin, David
2012-01-01
Background and aims The florist's gloxinia is a familiar houseplant in the Gesneriaceae, the botanical family that includes the African violet (Saintpaulia) and other ornamental species. The gloxinia's wild progenitor is Sinningia speciosa (Lodd.) Hiern, a Brazilian endemic. Although it has been cultivated for almost 200 years, little is known about the genetic diversity in S. speciosa, how the wild populations relate to one another or even where the cultivated forms originated. Using available wild collections, preliminary phenetic and phylogenetic investigations were conducted to elucidate the interspecific relationships within S. speciosa and to infer the origins of the cultivars. Methodology Amplified fragment length polymorphism (AFLP) analysis was applied to 24 accessions of S. speciosa (17 wild collections, seven cultivars) and one accession each of Sinningia guttata and Sinningia macrophylla. A maximum likelihood (ML) tree was also calculated from an alignment of the nuclear ribosomal internal transcribed spacer sequence from the same 26 accessions. Principal results Dice/UPGMA and principal coordinates analysis of the AFLP data partitioned S. speciosa into several distinct clusters, one of which included S. macrophylla. All cultivated ‘gloxinias’ grouped together in a major cluster with plants from Rio de Janeiro. The AFLP results were compared with a phylogenetic analysis of the ribosomal spacer region, which was informative in S. speciosa. The ML tree generally supported the AFLP results, although several clades lacked strong statistical support. Conclusions Independent analyses of two different data sets show that S. speciosa is a diverse species comprised of several lineages. Genetic distance estimates calculated from the AFLP data were positively correlated with geographic distances between populations, indicating that reproductive isolation could be driving speciation in this taxon. Molecular markers are under development for population genetic
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Energy Technology Data Exchange (ETDEWEB)
Taenzler, Dennis; Westerkamp, Meike [Adelphi Research, Berlin (Germany); Supersberger, Nikolaus; Ritthoff, Michael; Bleischwitz, Raimund [Wuppertal Institut (Germany)
2011-04-15
In Bolivia's western highlands, at an altitude of some 3,600 metres, lie the largest identified reserves of lithium in the world. According to the latest estimates by the United States Geological Survey (USGS), beneath the 10,000 square kilometres of what are the world's most extensive salt flats, the Salar de Uyuni, there are some 6 to 9 million tonnes of lithium (Roskill 2008 nach Angerer, Marscheider-Weidemann, Wendl et al. 2009, Jaskula 2010a). Exploitation of those reserves has not yet begun, though the Bolivian government has constructed a small 6 million US Dollar pilot plant on the edge of the Salar de Uyuni, and has some ambitious plans: ''Lithium is the hope not just for Bolivia but for all inhabitants of the planet'', stated Bolivia's President Evo Morales in February 2009 (Bajak/Valdez 2009). Bolivia is one of the poorest countries in Latin America. The production of lithium, and the revenues it can generate, represents a major development opportunity for the country. Lithium is a key constituent of lithium ion batteries. Already in widespread use in mobile phones, camcorders and laptops, they are also increasingly needed to power hybrid and electric vehicles, which is why many analysts regard lithium as one of the key raw materials in the move towards sustainable mobility in the post-oil era (National Research Council (U.S.) 2008, Angerer et al. 2009, Tahil 2007; Chameides 2009). As the demand for lithium rises and its importance grows, the question arises as to the risks and opportunities associated with it. The resource booms of the past did little to promote Bolivia's development and improve the standard of living of its population. Indeed, Bolivia has a long history of resource-related conflict and political instability. This report (3.3) therefore investigates the risks and opportunities associated with the establishment of industrial-scale lithium production in Bolivia. Its analysis follows on from reports 1 and 2, illustrating and expanding upon
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Energy Technology Data Exchange (ETDEWEB)
Taenzler, Dennis; Westerkamp, Meike [Adelphi Research, Berlin (Germany); Supersberger, Nikolaus; Ritthoff, Michael; Bleischwitz, Raimund [Wuppertal Institut (Germany)
2011-04-15
In Bolivia's western highlands, at an altitude of some 3,600 metres, lie the largest identified reserves of lithium in the world. According to the latest estimates by the United States Geological Survey (USGS), beneath the 10,000 square kilometres of what are the world's most extensive salt flats, the Salar de Uyuni, there are some 6 to 9 million tonnes of lithium (Roskill 2008 nach Angerer, Marscheider-Weidemann, Wendl et al. 2009, Jaskula 2010a). Exploitation of those reserves has not yet begun, though the Bolivian government has constructed a small 6 million US Dollar pilot plant on the edge of the Salar de Uyuni, and has some ambitious plans: ''Lithium is the hope not just for Bolivia but for all inhabitants of the planet'', stated Bolivia's President Evo Morales in February 2009 (Bajak/Valdez 2009). Bolivia is one of the poorest countries in Latin America. The production of lithium, and the revenues it can generate, represents a major development opportunity for the country. Lithium is a key constituent of lithium ion batteries. Already in widespread use in mobile phones, camcorders and laptops, they are also increasingly needed to power hybrid and electric vehicles, which is why many analysts regard lithium as one of the key raw materials in the move towards sustainable mobility in the post-oil era (National Research Council (U.S.) 2008, Angerer et al. 2009, Tahil 2007; Chameides 2009). As the demand for lithium rises and its importance grows, the question arises as to the risks and opportunities associated with it. The resource booms of the past did little to promote Bolivia's development and improve the standard of living of its population. Indeed, Bolivia has a long history of resource-related conflict and political instability. This report (3.3) therefore investigates the risks and opportunities associated with the establishment of industrial-scale lithium production in Bolivia. Its analysis follows on from reports
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Directory of Open Access Journals (Sweden)
Martha Pérez
2003-06-01
superficie abaxial sólo están los haces de fibras y hacia la superficie adaxial hay haces vasculares y haces fibrosos. Las fibras, en las tres especies son alargadas, de extremos aguzados, no ramificadas ni septadas. En los haces vasculares se observan de uno a tres vasos amplios del metaxilema, siendo S. yapa la que los tiene de mayor diámetro. Los haces vasculares están rodeados por gruesas vainas de fibras. El pecíolo tiene una estructura anatómica similar a la de la lámina y se caracteriza por los numerosos haces vasculares y haces de fibras esparcidos en el tejido parenquimatoso. La estructura histológica de lámina y pecíolo reflejan cualidades de resistencia y flexibilidad que las hacen adecuadas para ser utilizadas en el techado de viviendas rurales y otras construcciones.This paper describes the leaf anatomy of Sabal mauritiiformis (Karst. Griseb. & H. Wendl., Sabal mexicana Mart. and Sabal yapa Wright ex Becc., three of the four most representative species of the Yucatán Península, in Mexico. These species are locally used: in the roofing of traditional homes, as food (fruits and apical buds, and in the production of hats, brooms and handicrafts. Leaf samples were collected in secondary growth of lower montane rainforest in the state of Quintana Roo and in two home gardens in the state of Yucatán. Herbarium samples were obtained, and samples of blade and petiole were fixed in formaline-acetic acid-alcohol. Cross incisions were made on the blade and petiole, and were dyed with safranin and toluidine blue O.The results show that S. mauritiiformis and S. yapa are morphologically alike: both are tall, slim palm trees; the leaf in S. mauritiiformis is a shorter palm-like structure compared with the other two species. The shape of the main nerve, as seen in cross section, is rectangular in the three species. The hastula in the three species is acuminate and adaxial. The foliar anatomic structure is similar in the three species, although there are some