radial spoke protein: Topics by WorldWideScience.org

Radial Halbach Magnetic Bearings

In this note we describe a support structure which will hold detector systems (ITS, TPC, TOF) inside the large solenoid. An overall view of ALICE detector is shown on Fig.1. The support structure consists of 2 rings which fixed to an iron yoke and spokes which connect TPC and TOF to these rings. The ITS is connected by spokes to TPC end caps. Between coils there are 7cm spacings which will allow to fix rings to the yoke this support structure will allow to assemble ITS, TPC and TOF together (Fig.2) somewhere on the surface and then transport to the pit and install in the magnet. Two rings (1) are connected with four bars (2). On the bottom bars there are rolls (3) which will allow smoothly insert detectors into the magnet. Spokes (4) are uniformly distributed.

1993-01-01

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Friends of the Earth: Bike repair for dummies: Green Blog

Radial Halbach magnetic bearings have been investigated as part of an effort to develop increasingly

2009-01-01

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Wastenet

... My road safety has come a long way since a bus driver yelled 'Go and read the Highway Code!' at me in my first wobbly week. I've got over my bike theft paranoia and happily leave my trusty steed shackled to lampposts around London. And I've figured out the optimum skirt-length for pedaling - neither flasher short nor so long it gets stuck in the oily spokes. But ...

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High resolution scanning electron microscopy of plasmodesmata.

DEVICE FOR TISSUE CULTURE, PROTEIN EXTRACTION AND PROTEIN CONCENTRATION

Symplastic transport occurs between neighbouring plant cells through functionally and structurally dynamic channels called plasmodesmata (PD). Relatively little is known about the composition of PD or the mechanisms that facilitate molecular transport into neighbouring cells. While transmission electron microscopy (TEM) provides 2-dimensional information about the structural components of PD, 3-dimensional information is difficult to extract from ultrathin sections. This study has exploited high-resolution scanning electron microscopy (HRSEM) to reveal the 3-dimensional morphology of PD in the cell walls of algae, ferns and higher plants. Varied patterns of PD were observed in the walls, ranging from uniformly distributed individual PD to discrete clusters. Occasionally the thick walls of the giant alga Chara were fractured, revealing the surface morphology of PD within. External structures such as spokes, spirals and mesh were observed surrounding the PD. ...

2011-05-28

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J-STORE (Japan)

Full Text Available

2007-12-28

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Protein-protein interactions and structural analysis of citrus proteins involved in transcriptional control during citrus canker disease

A brief historical development of classical mathematics before the Renaissance

No abstract prepared.

2010-07-01

8

British Library Electronic Table of Contents (United Kingdom)

'If you wish to foresee the future of mathematics our proper course is to study the history and present condition of the science.' Henri Poincare 'It is India that gave us the ingenious method of expressing all numbers by ten symbols, each symbol receiving a value of position, as well as an absolute value. We shall appreciate the grandeur of the achievement when we remember that it escaped the genius of Archimedes and Apollonius.' P.S. Laplace 'The Greeks were the first mathematicians who are still 'real' to us today. Oriental mathematics may be an interesting curiosity, but Greek mathematics is the real thing. The Greek first spoke of a language which modern mathematicians can understand.' G.H. Hardy This article deals with a short history of mathematics and mathematical scientists during...

2011-01-01

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Heat shock proteins as emerging therapeutic targets

Quantitation of Antibody to Non-Hemagglutinating Viruses by Single Radial Hemolysis: Serological Test for Human Coronaviruses

Chaperones (stress proteins) are essential proteins to help the formation and maintenance of the proper conformation of other proteins and to promote cell survival after a large variety of environmental...Full Text Available

2005-11-01

10

Magnetic resonance imaging findings in 46 elbows with a radial head fracture

A single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. With the human coronaviruses as models, this test utilizes the binding properties...Full Text Available

1977-06-01

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Influence of geometry and operating conditions of a centrifugal compressor stage on the radial gas force

Background and purposeRadial head fractures are common, and may be associated with other injuries of clinical importance. We present the results of a standard additional MRI scan...Full Text Available

2010-06-01

12

British Library Electronic Table of Contents (United Kingdom)

The influence of geometry and operating conditions of the centrifugal compressor stage on the radial gas force is determined on the basis of the theoretical method and calculation program using experimental boundary conditions.

2011-01-01

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An analytic representation of the radial distribution of dose from energetic heavy ions in water, Si, LiF, and NaI

Symmetric surface waves in cylindrical waveguide structures filled by radially non-uniform collisional plasma

An earlier representation of the radial distribution of dose about the path of a heavy ion in liquid water is modified and extended to include silicon, lithium fluoride, and sodium iodide. 6 refs., 5 figs., 1 tab.

1989-09-01

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Development of radial-flow type internally oil-cooled oil-filled cable

... Union (INTAS), Brussels (Belgium) Science and Technology Center in Unkraine,

2006-09-11

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Analysis of the performance of fuel cells BWR with a single enrichment and radial distribution of enrichments

A radial-flow type internally oil-cooled oil-filled cable has been developed. This system is characterized in that the oil flows radially through the paper insulation. The thermal resistance of the insulation is equivalently decreased by the radial-flow. The decreased thermal resistance as well as the cooling effect of the oil flow in the central oil duct and the outer oil duct under an aluminum sheath increase the current capacity. A calculation method of cooling characteristics is introduced. The stability of the cable and accessories was confirmed through initial electrical tests and a long-term field test.

1988-01-01

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Hyperfine anomaly measurements in francium isotopes and the radial distribution of neutrons

The efficient use of the fuel is one of the objectives in the assemblies design of type BWR. The present tendency in the assemblies design of type BWR is through a radial distribution of enrichments. The present work has like object showing the because of this decision, for what a comparison of the neutronic performance of two fuel cells with the same enrichment average but one of them with radial distribution of enrichment and the other with a single enrichment equal to the average. The cells were analyzed with the CASMO-4 code and the obtained results of the behavior of the neutron flow and the power sustain the because of the radial distribution of enrichments. (Author)

2008-07-06

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Examination of graphitization products of coal by using radial distribution function method

We have performed precision measurements in a magneto-optical trap of the 7P1/2 hyperfine structure of the isotopes 209-210Fr. The ratio of these hyperfine constants to the previously measured 7S1/2 ground state values reveals a significant hyperfine anomaly. This anomaly results from the different radial dependence of the electron density in the two atomic levels. The measurements are sensitive to changes in the radial distribution of the neutron magnetism.

1999-09-01

18

Radial velocities, dynamics of stars and nebulosities with GAIA and VLT-GIRAFFE

The radial distribution function (RDF) derived from wide angle X-ray diffraction patterns gives additional information about the spatial distribution of atoms. Their radial distances and relative average coordination numbers can be calculated using appropriate mathematical procedures. 7 refs.

1991-01-01

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Axial and radial distribution of neutron fluxes in the irradiation channels of the Ghana Research Reactor-1 using foil activation analysis and Monte Carlo

This document is divided in two parts. The first part deals with the radial velocities (RV) distributions for B-type stars and nebulosities observed with the VLT-GIRAFFE in the Large and Small Magellanic Clouds towards the open clusters NGC2004 and NGC330. Thanks to the resolution of GIRAFFE spectra, we found that the RV distribution for the nebulosities in the LMC is bi-modal. This bi-modality can be interpreted, in term of dynamics, by the expansion of the LMC4 superbubble. The second part deals with the GAIA space mission and the determination of the radial velocities by using Radial Velocity Spectrometer (RVS) spectra. The methods to determine the radial velocities are presented as well as preliminary results on simulated RVS spectra.

2008-01-01

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Charged nanoparticles as protein delivery systems: A feasibility study using lysozyme as model protein

The Monte-Carlo method and experimental methods were used to determine the neutron fluxes in the irradiation channels of the Ghana Research Reactor -1. The MCNP5 code was used for this purpose to simulate the radial and axial distribution of the neutron fluxes within all the ten irradiation channels. The results obtained were compared with the experimental results. After the MCNP simulation and experimental procedure, it was observed that axially, the fluxes rise to a peak before falling and then finally leveling out. Axially and radially, it was also observed that the fluxes in the centre of the channels were lower than on the sides. Radially, the fluxes dip in the centre while it increases steadily towards the sides of the channels. The results have shown that there are flux variations within the irradiation channels both axially and radially. (au)

2009-01-01

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DEFF Research Database (Denmark)

Udgivelsesdato: 2008/5

2008-01-01

22

The Stability and Formation of Native Proteins from Unfolded Monomers Is Increased through Interactions with Unrelated Proteins

Localization of Reversion-Induced LIM Protein (RIL) in the Rat Central Nervous System

The intracellular concentration of protein may be as high as 400 mg per ml; thus it seems inevitable that within the cell, numerous protein-protein contacts are constantly occurring. A basic biochemical...Full Text Available

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DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle

Reversion-induced LIM protein (RIL) is a member of the ALP (actinin-associated LIM protein) subfamily of the PDZ/LIM protein family. RIL serves as an adaptor protein and seems to regulate cytoskeletons....Full Text Available

2009-02-28

24

Salt modulates the stability and lipid binding affinity of the adipocyte lipid-binding proteins

The DNA double-strand break (DSB) damage response induced by high energy charged particles on lung fibroblast cells embedded in a 3-dimensional (3-D) collagen tissue equivalents was investigated using antibodies to the DNA damage response proteins gamma-histone 2AX (#gamma#-H2AX) and phosphorylated DNA-PKcs (p-DNA-PKcs). 3-D tissue equivalents were irradiated in positions across the linear distribution of the Bragg curve profiles of 307.7 MeV/nucleon, 556.9 MeV/nucleon, or 967.0 MeV/nucleon "5"6Fe ions at a dose of 0.30 Gy. Patterns of discrete DNA damage streaks across nuclei or saturated nuclear damage were observed, with saturated nuclear damage being more predominant as samples were positioned closer to the physical Bragg peak. Quantification of the DNA damage signal intensities at each distance for each of the examined energies revealed a biological Bragg curve profile with a pattern of DNA damage intensity similar to the physical Bragg curve for the ...

2010-03-01

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Protein misfolding disorders and macroautophagy

Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding protein that is

2003-01-01

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Misfolded Proteins and Retinal Dystrophies

A large group of diseases, termed protein misfolding disorders, share the common feature of the accumulation of misfolded proteins. The possibility of a common mechanism underlying either the pathogenesis...Full Text Available

2011-04-01

27

CAAX-box protein, prenylation process and carcinogenesis

Many mutations associated with retinal degeneration lead to the production of misfolded proteins by cells of the retina. Emerging evidence suggests that these abnormal proteins cause cell death...Full Text Available

2010-01-01

28

Radial distribution of superthermal electrons measured with ECE (abstract)

CAAX proteins are widely involved in global cellular functions such as proliferation, differentiation, and carcinogenesis. As an important modulator of biological activity, signal transduction via protein...Full Text Available

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Symmetric Surface Waves in Cylindrical Waveguide Structures Filled by Radially Non-uniform Collisional Plasma

To aid in the study of lower hybrid current drive as a means of current profile control, the radial profile and velocity distribution of the fast current-carrying electrons and their time evolution must be known. As part of the recent effort to understand this fast electron transport, a diagnostic has been installed on PBX-M to measure the electron cyclotron emission from the fast electrons. An oblique horizontal view of upshifted cyclotron emission in the midplane can give radial as well as velocity space information about the fast electrons. Emission in X mode frequencies refracted by the right-hand cutoff is radially localized. The observed emission at a specific frequency comes only from the radial region r_t_u_r_n=#0. Emission measured by this new diagnostic has been found to be radially localized as predicted. It is also expected that localized moments of the distribution ...

0854-01-01

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Orbit of the double-mode cepheid Y Carinae

This report is devoted to the investigation of the influence of electron collisions and radial non-uniformity of plasma density on phase characteristics, spatial attenuation and wave field structure of slow symmetric electromagnetic waves that propagate along cylindrical waveguide structure. It has been shown that collision rate and radial non-uniformity of plasma density for various parameters of waveguide structure and dielectric affect essentially on the wave characteristics and consequently, on the parameters of gas discharge that is sustained by this wave. The results obtained are of large importance for the construction of the theory of gas discharges that are sustained by the surface electromagnetic waves.

2006-01-01

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Geometry changes transient transport in plasmas

The double-mode cepheid Y Car has been found to have a variable centre-of-mass velocity. Though the observations did not cover a cycle, an orbital period of about 400-600 days was estimated. Radial-velocity observations of this star have now been continued in order to derive the orbital elements. Observations were made with the photoelectric radial-velocity spectrophotometer at the coude focus of the 1.88-m reflector at Sutherland. The velocity system was standardized by frequent nightly observations of stars in the Mount Wilson catalogue with 'a'-quality radial velocities.

1983-06-01

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Some features of the atomic radial-distribution functions of metal glasses

Role of ballooning effect in toroidal plasmas on the transient transport problems is investigated. Due to the mode localization along the magnetic field line, a meso scale appears in a radial correlation length of fluctuating fields. This scale length introduces the interference of the gradient and flux in different radial locations. For the fluctuation which gives the gyro-Bohm-like diffusion in a stationary state, this long radial correlation of the fluctuating field causes a fast propagation of response against a rapid transient perturbation. Upper bound of transient thermal diffusivity is derived. (author)

2001-06-01

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Semi Annual Progress Report on BLADE END WALL FLOWS IN COMPRESSORS ...

This paper attempts to explain the peculiarities of the radial-distribution function of metal glasses without involving ideas of the amorphous structure. On a computer, the radial atomic density for a spherical eutectic single crystal of the composition Fe/sub 84/C/sub 16/ of radius 15 A formed by alternating small crystals of e-Fe and Fe/sub 3/C of cubic form with the edge of the cube ca 10 A. For the sake of clarity, the diagram of such a quasisingle crystal is shown and has been given a cubic boundary. The change in the relationship between the heights of the subpeaks of the second maximum of the radial distribution function of atoms in the Fe-B glasses with a change in the concentration of boron can be explained by the change in the space group of the Fe/sub 3/B metastable boride which is formed in this system.

1986-09-01

34

Radial distribution of the electric field and the space charge distribution in a low pressure molecular gas plasma

Eckert, B., "Axial Kompressoren und Radial Kompressoren," Springer. Verlag, Berlin, 1953. 3. Lebot, Y., et al., "Theoretical and Experimental Determination ...

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Loop frame of reference based three-phase power flow for unbalanced radial distribution systems

German (15 Jun 1974). Switzerland Kocian, P. Ecole Polytechnique Federale,

1974-06-01

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First derivative of the hard-sphere radial distribution function at contact

This paper introduces a novel three-phase power flow approach for unbalanced radial distribution systems. The proposed approach is developed based on the loop frame of reference, rather than the traditional bus frame of reference. On the basis of the loop frame of reference, a simple direct iterative method in impedance form is applied. Basic graph theory and injection current technique are also applied in the proposed approach. The clear theoretical foundation and the simple topology of the radial distribution network make the proposed method efficient and reliable. To demonstrate the better convergence performance and the efficiency of the proposed approach, four three-phase IEEE test feeders are used for comparisons. The test results show that the proposed method has robust convergence characteristics and high performance, especially for large-scale radial distribution systems. (author)

2010-07-15

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Comparison of the SASSYS/SAS4A radial core expansion reactivity feedback model and the empirical correlation for FFTF

Molecular dynamics simulations have been carried out of the radial distribution function of the hard sphere fluid for a range of densities in the equilibrium fluid and just into the metastable region. The first derivative of the hard-sphere radial distribution function at contact was computed and its density dependence fitted to a simple analytic form. Comparisons were made with semi-empirical formulae from the literature, and of these the formula proposed by Tao et al (1992 Phys. Rev. A 46 8007) was found to be in best agreement with the simulation data, although it slightly underestimates the derivative at the higher packing fractions in excess of about 0.45. Close to contact, within a few per cent of the particle diameter, the radial distribution function can be represented well by a second order polynomial. An exponential function, which has some useful analytic features, can also be applied in this region.

2006-08-16

38

RIN4-like proteins mediate resistance protein-derived soybean defense against Pseudomonas syringae

The present emphasis on inherent safety for LMR designs has resulted in a need to represent the various reactivity feedback mechanisms as accurately as possible. The dominant negative reactivity feedback has been found to result from radial expansion of the core for most postulated ATWS events. For this reason, a more detailed model for calculating the reactivity feedback from radial core expansion has been recently developed for use with the SASSYS/SAS4A Code System. The purpose of this summary is to present an extension to the model so that it is more suitable for handling a core restraint design as used in FFTF, and to compare the SASSYS/SAS4A results using this model to the empirical correlation presently being used to account for radial core expansion reactivity feedback to FFTF.

1987-01-01

39

Protein S inherited qualitative deficiency: novel mutations and phenotypic influence.

Resistance (R) protein mediated recognition of pathogen avirulence effectors triggers signaling that induces a very robust form of species-specific immunity in plants. The soybean Rpg1-b protein mediates...Full Text Available

2010-11-01

40

Overcoming the challenges of membrane protein crystallography

PMID:20880255

2010-12-01

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41

Effect of Garlic on the Digestion of Beef Protein during Storage

Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane...Full Text Available

2008-10-01

42

A modified immunoblot method to identify substrates of protein kinases

Full Text Available

1991-10-01

43

Radial distribution of dose and cross-sections for the inactivation of dry enzymes and viruses

Full Text Available

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Radial distribution of bonded fission gas in mixed carbide fuel pins

A new semi-empirical algorithm for the radial distribution of dose is compared with available data. The algorithm is used to calculate the inactivation cross section for dry enzymes and viruses using an extended target model of a 1-hit detector. Agreement with data is at about the 15% level, approximating the precision of the data itself. (author).

1985-05-01

45

Radial distribution functions of liquid Na and Cs

The fission gas xenon bonded in bubbles, in pore, and in the lattice of mixed carbide fuels is measured by electron-probe microanalysis. Radial xenon distribution and release curves are determined and are calibrated by gas chromatography of the bonded fission gas and by burnup analysis in the respective pin sections of the irradiation experiments FR2 6A and 6C, Mol 11/K 2, and DFR 330/1. The results are correlated to the microstructure of the fuel, bonding medium, temperature, and burnup. (Auth.).

1979-01-01

46

Radial distribution functions of amorphous silicon

Radial distribution functions of liquid sodium and caesium at 100"0C have been calculated by the method of molecular dynamics with interionic pair potentials derived from Heine-Abarenkov-Shaw type model potential. The results were found to be in good agreement with recent experimental data. (Auth.).

1978-01-01

47

Radial distribution function and second virial coefficient for interacting bosons

Substantial changes in the radial distribution function of amorphous Si films have been observed in neutron-diffraction studies. The spectra indicate changes in short-range order associated with an approx.11% modification in the bond-angle distribution width. The results allow the first direct comparison of structural and vibrational Raman probes of variations in local order in thin-film amorphous solids. Good agreement is obtained between the measured bond-angle variation and that based on Raman estimates.

1989-03-15

48

Optimal selection of capacitors for radial distribution systems using a genetic algorithm

The radial distribution function and the second virial coefficient of interacting bosons have been studied. The second virial coefficient has been deduced theoretically and is in good agreement with experimental values. The third virial coefficient has been calculated from the experimental values of the pressure. (Auth.).

1976-01-01

49

Amplitude-phase formula for the S-matrix derived from invariants of the reduced first-order radial Dirac equation

In this paper, a new design methodology for determining the size, location, type and number of capacitors to be placed on a radial distribution system is presented. The objective is to minimize the peak power losses and the energy losses in the distribution system considering the capacitor cost. A sensitivity analysis based method is used to select the candidate locations for the capacitors. A new optimization method using a Genetic Algorithm is proposed to determine the optimal selection of capacitors. Test results have been presented along with the discussion of the algorithm.

1994-08-01

50

Amplitude-phase formula for the S-matrix derived from invariants of the reduced first-order radial Dirac equation

A formula for calculating the Dirac S-matrix for central Lorentz scalar and vector potentials is derived by use of a new amplitude-phase method. The derivation also makes use of certain invariants of the reduced 2-spinor radial Dirac equations.

2008-12-15

51

Kinetics of spherulite formation and growth: Salt and protein concentration dependence on proteins b-lactoglobulin and insulin

A formula for calculating the Dirac S-matrix for central Lorentz scalar and vector potentials is derived by use of a new amplitude-phase method. The derivation also makes use of certain invariants of the reduced 2-spinor radial Dirac equations.

2008-12-01

52

British Library Electronic Table of Contents (United Kingdom)

Proteins aggregated into spherulite structures of amyloid fibrils have been observed in patients with certain brain diseases such as Alzheimers and Parkinsons. The conditions under which these protein spherulites form and grow are not currently known. In order to illuminate the role of environmental factors on protein spherulites, this research aims to explore the kinetics and mechanisms of spherulite formation and growth, as monitored by optical microscopy, in a range of salt concentrations, and initial protein concentrations for two model proteins: bovine b-lactoglobulin and insulin. These two proteins are significantly different in their size and fibril growth rate, but both of these proteins have been shown previously to form amyloid fibrils and spherulites under low pH conditions. The...

2009-01-01

53

Pressure-induced structural transitions in multi-walled carbon nanotubes

British Library Electronic Table of Contents (United Kingdom)

We demonstrate a novel cross-sectional deformation, called the radial corrugation, of multi-walled carbon nanotubes (MWNTs) under hydrostatic pressure. Theoretical analyses based on the continuum elastic approximation have revealed that MWNTs consisting of more than ten concentric walls undergo elastic deformations at critical pressure Formula Not Shown , above which the circular shape of the cross-section becomes radially corrugated. Various corrugation modes have been observed by tuning the innermost tube diameter and the number of constituent walls, which is a direct consequence of the core-shell structure of MWNTs. Cross-sectional views of MWNT under high hydrostatic pressure: elliptic deformation with the mode index n = 2 (left), and radial corrugations with n = 5 (center), and n = 6 ...

2009-01-01

54

Possible control scenario of radial electric field by loss-cone-particle injection into helical device

Numerical simulation of the flow field in a high specific speed radial fan; Numerische Simulation der Stroemung in einem Radialventilator mit hoher spezifischer Drehzahl

The possibility of controlling the radial electric field of toroidal plasmas by injecting high energy electrons along the reversible loss cone orbit of the helical magnetic traps is investigated. It is well known that the radial electric field plays an important role in the confinement improvement scenario especially in the low collisional regime under the physics picture of neoclassical theory. For this purpose, it is made clear that the most suitable particles are transit particles, which show a transition from helically trapped orbits to blocked ones. It is also found that a parallel AC electric field launched from outside assists this transition and makes it possible for particles to penetrate deeply into the plasma. In addition we clarify that the viscosity of the plasma coupled with the helical field configuration provide a bifurcation of plasma states and its stable solution results in confinement improvement. (author)

1999-08-01

55

Measurement of the dark matter velocity anisotropy profile in galaxy clusters

The present paper describes the application of the commercial CFD-code FLUENT 6.1 to the numerical simulation of the flow field in a high specific speed radial fan. Two-dimensional as well as three-dimensional computations are performed using the steady 'Frozen-Rotor method' as well as the unsteady 'Sliding-Mesh method'. The predicted performance curves of the radial fan are compared with the results obtained from measurements on a test stand. Due to the relatively low computation times, the 'Frozen-Rotor method' can be used routinely for design purposes. The results of this approach can be improved if the leakage flow through the gap between the inlet nozzle and the impeller is taken into account. (orig.)

2005-11-01

56

Erosive radially-slotted discharge in sheet current mode

Dark matter particles form halos that contribute the major part of the mass of galaxy clusters. The formation of these cosmological structures have been investigated both observationally and in numerical simulations, which have confirmed the existence of a universal mass profile. However, the dynamic behaviour of dark matter in halos is not as well understood. We have used observations of 16 equilibrated galaxy clusters to show that the random velocities of dark matter particles are larger on average along the radial direction than along the tangential, and that the magnitude of this velocity anisotropy is radially varying. Our measurement implies that the collective behaviour of dark matter particles is fundamentally different from that of normal particles and the radial variation of the anisotropy velocity agrees with the predictions of numerical simulation.

2008-01-01

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Electrical drilling string separator

New non-stationary non-contracted form of the erosive radially-slotted discharge as a thin round sheet with the current of the azimuth direction have been discovered, its existence beings stipulated by a radial transport-wave fluxes. Characteristic features of this discharge is self-confinement of the discharge current magnitude, corresponding decrease of the current pulse duration and occurrence of an energy and substance ejection with rather unusual properties. Measurements of kinetics of the discharge current, the plasma radiation intensity and an electrical probe signal, as well as the transmission electron microscope investigations of characteristic aerodisperse aggregates arising the erosive phase, have been carried out. The probe signal duration was about 10 times greater than that of the current; its kinetics was complicated suggesting existence in the slot of two components with fundamentally different properties and states of the ...

2001-01-01

58

Acoustic resonances in two-dimensional radial sonic crystal shells

The separator can be used for electrical separation of the drilling string used as the channel of communication with transmission of face information. It contains upper and lower metal conductors electrically insulated from each other by an insulator made of layered polymer composite material. In order to improve reliability of the connection of the conductors to the insulator and reduce the metal consumption on the ends of the conductors that come into contact with the insulator there are graduated niches with radial projections. The latter form jointly with the layers of the insulator a crown-radial-multiple stage undetachable connection. The niches decreases from the outer diameter of the conductors to the inner. The insulator has additional layers made of high-module fibers of carbon or boron which cover the radial projections in stages.

1983-01-01

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A novel approach for measuring the radial distribution of charge in a heavy ion track

Radial sonic crystals (RSC) are fluidlike structures infinitely periodic along the radial direction that verify the Bloch theorem and are possible only if certain specially designed acoustic metamaterials with mass density anisotropy can be engineered (see Torrent and Sanchez-Dehesa 2009 Phys. Rev. Lett. 103 064301). A comprehensive analysis of two-dimensional (2D) RSC shells is reported here. A given shell is in fact a circular slab with a central cavity. These finite crystal structures contain Fabry-Perot-like resonances and modes strongly localized at the central cavity. Semi-analytical expressions are developed to obtain the quality factors of the different resonances, their symmetry features and their excitation properties. The results reported here are completely general and can be extended to equivalent 3D spherical shells and to their photonic counterparts.

2010-07-15

60

Whey protein isolate attenuates strength decline after eccentrically-induced muscle damage in healthy individuals

The energy deposited by the passage of a single, energetic, heavy-ion through a semiconductor produces dense electron-hole (eh) pair concentrations near the ion trajectory. The size, shape, and charge density of an ion track represent critical parameters for many models of single event phenomena. The authors describe the design and uses of possible semiconductor test structures for measuring the initial radial distribution of charge and subsequent charge transport in a high energy, heavy-ion track. Numerical simulations show how the test structure can resolve different radial distributions of charge within an ion track. The test structure simulations also show the importance of accurately representing ion track structure in single event effects simulations.

1994-07-18

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61

Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

62

Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis

Known vertebrate GATA proteins contain two zinc fingers and are required in development, whereas invertebrates express a class of essential proteins containing one GATA-type zinc finger. We isolated...Full Text Available

2001-04-02

63

The protein storage vacuole

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...Full Text Available

1998-04-01

64

The p14 FAST Protein of Reptilian Reovirus Increases Vesicular Stomatitis Virus Neuropathogenesis?

Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage...Full Text Available

2001-12-10

65

Strategies for the uses of lanthanide NMR shift probes in the determination of protein structure in solutio. Application to the EF calcium binding site of carp parvalbumin.

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

2009-01-01

66

Stage-specific protein synthesis during early embryogenesis in Drosophila melanogaster.

The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available

1980-10-01

67

Serum protein binding and the role of increased alpha 1-acid glycoprotein in moderately obese male subjects.

The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis. Of the 261 proteins scored, 68 (26%) show dramatic changes in...Full Text Available

1983-01-01

68

Selecting Folded Proteins from a Library of Secondary Structural Elements

Serum protein and lipid concentrations as well as the serum protein binding of propranolol, diazepam and phenytoin were measured in normal weight and obese volunteers. Concentrations of alpha 1-acid...Full Text Available

1984-12-01

69

Regulation of the Actin Cytoskeleton by an Interaction of IQGAP Related Protein GAPA with Filamin and Cortexillin I

A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands...Full Text Available

2008-01-09

70

Regulation of ROMK1 Channels by Protein-tyrosine Kinase and -tyrosine Phosphatase*

Filamin and Cortexillin are F-actin crosslinking proteins in Dictyostelium discoideum allowing actin filaments to form three-dimensional networks. GAPA, an IQGAP related protein, is...Full Text Available

71

Purification, characterization and binding interactions of the Chinese-cobra (Naja naja atra) serum antitoxic protein CSAP.

We have used the two-electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK1 channels by protein-tyrosine phosphatase (PTP) and protein-tyrosine...Full Text Available

2001-03-09

72

Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.

The characterization of the single-chain protein in Chinese-cobra (Naja naja atra) blood serum, which yields strong specific protection against the venom of the same snake, is reported. The protein,...Full Text Available

1993-07-15

73

Protein Damage by Reactive Electrophiles: Targets and Consequences

A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts...Full Text Available

1978-09-01

74

ProMoST: A tool for calculating the pI and molecular mass of phosphorylated and modified proteins on 2 dimensional gels

It has been sixty years since the Millers first described the covalent binding of carcinogens to tissue proteins. Protein covalent binding was gradually overshadowed by the emergence of DNA...Full Text Available

2008-01-01

75

Partial Characterization of Cadmium-Binding Protein from Roots of Tomato ¹

Protein modifications such as phosphorylation are often studied by two-dimensional gel electrophoresis since the perturbation in the protein’s pI value is readily detected by this method....Full Text Available

2009-01-01

76

PDBe: Protein Data Bank in Europe

Cd-binding protein was extracted from tomato roots and purified on QAE-Sephadex A-25 and on Sephadex G-75 in 1 molar KCl buffer. The protein preparation was light brown and contained predominantly Cd...Full Text Available

1986-07-01

77

Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging

The Protein Data Bank in Europe (PDBe; pdbe.org) is actively involved in managing the international archive of biomacromolecular structure data as one of the partners in the Worldwide Protein Data Bank...Full Text Available

2011-01-01

78

The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA binding. To further...Full Text Available

1998-03-01

79

NASCENT: An automatic protein interaction network generation tool for non-model organisms

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this...Full Text Available

1994-05-24

80

Modular coherence of protein dynamics in yeast cell polarity system

Large quantity of reliable protein interaction data are available for model organisms in public depositories (e.g., MINT, DIP, HPRD, INTERACT). Most data correspond to experiments with the proteins...Full Text Available

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81

Mammalian end binding proteins control persistent microtubule growth

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

2011-05-03

82

How DNA coiling enhances target localization by proteins

End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles of the three mammalian EBs in controlling microtubule...Full Text Available

2009-03-09

83

Expression and Characterization of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase PknB

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

2008-10-14

84

Ethylene Regulates Monomeric GTP-Binding Protein Gene Expression and Activity in Arabidopsis1

PknB is a member of the newly discovered eukaryotic-like protein serine/threonine kinase (PSTK) family of proteins. The pknB gene was cloned and expressed in Escherichia coli....Full Text Available

1999-11-01

85

Effect of Protein Binding on the Pharmacological Activity of Highly Bound Antibiotics?

Ethylene rapidly and transiently up-regulates the activity of several monomeric GTP-binding proteins (monomeric G proteins) in leaves of Arabidopsis as determined by two-dimensional gel electrophoresis...Full Text Available

2003-04-01

86

Coat protein gene duplication in a filamentous RNA virus of plants.

During antibiotic drug development, media are frequently spiked with either serum/plasma or protein supplements to evaluate the effect of protein binding. Usually, previously reported serum or plasma...Full Text Available

2008-11-01

87

Cardiac Myosin Is a Substrate for Zipper-interacting Protein Kinase (ZIPK)*

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

1992-10-01

88

Biosynthesis and characterization of rabbit tooth enamel extracellular-matrix proteins.

Zipper-interacting protein kinase (ZIPK) is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain...Full Text Available

2010-02-19

89

Being a binding site: Characterizing residue composition of binding sites on proteins

Tooth enamel biomineralization is mediated by enamel proteins synthesized by ameloblast cells. Two classes of proteins have been described: enamelins and amelogenins. In lower vertebrates the absence...Full Text Available

1988-05-01

90

Analyzing the simplicial decomposition of spatial protein structures

The Protein Data Bank contains the description of more than 45,000 three-dimensional protein and nucleic-acid structures today. Started to exist as the computer-readable depository of crystallographic...Full Text Available

91

An Analysis of the Subunit Structure of the Crystalloid Protein Complex from Castor Bean Endosperm 1

BackgroundThe fast growing Protein Data Bank contains the three-dimensional description of more than 45000 protein- and nucleic-acid structures today. The large majority of the data...Full Text Available

92

Alpha-Hemoglobin-Stabilizing Protein: An Erythroid Molecular Chaperone

Chromatographic and electrophoretic studies have shown that the subunits of the crystalloid protein, isolated from mature castor bean (Ricinus communis L. cv Hale) seed endosperm protein...Full Text Available

1983-06-01

93

The structure of receptor-associated protein (RAP)

Alpha-hemoglobin-stabilizing protein (AHSP) is an erythroid-specific protein that acts as a molecular chaperone for the free α chains of hemoglobin. Evidence strongly suggests...Full Text Available

2011-01-01

94

Phospholemman: A Novel Cardiac Stress Protein

The receptor-associated protein (RAP) is a molecular chaperone that binds tightly to certain newly synthesized LDL receptor family members in the endoplasmic reticulum (ER) and facilitates their delivery...Full Text Available

2007-08-01

95

PDBpaint, a visualization webservice to tag protein structures with sequence annotations

Phospholemman (PLM), a member of the FXYD family of regulators of ion transport, is a major sarcolemmal substrate for protein kinases A and C in cardiac and skeletal muscle. In the heart, PLM...Full Text Available

2010-08-01

96

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

2011-01-01

97

Male Reproductive Proteins and Reproductive Outcomes

Biogenesis of the Protein Storage Vacuole Crystalloid

Male reproductive proteins (MRPs), associated with sperm and semen, are the moieties responsible for carrying male genes into the next generation. Evolutionary biologists have focused on their...Full Text Available

2008-06-01

98

Alpha-1 antitrypsin is a potential biomarker for hepatitis B

We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter...Full Text Available

2000-08-21

99

9902631 - Lysozyme - NASA

BackgroundFunction exertion of specific proteins are key factors in disease progression, thus the systematical identification of those specific proteins is a prerequisite to understand...Full Text Available

100

Perturbations of the Plebanski metric in general relativity. II. Perturbations of the fermion field.

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

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101

Comparison of the SASSYS/SAS4A radial core expansion reactivity feedback model and the empirical correlation for the FFTF

We obtain the radial Dirac equations for the Plebanski metric and transform these equations into one-dimensional wave equations. Finally we conclude with the result that the electron and the neutrino fields are not superradiant.

1986-09-01

102

The root cause analysis of 9DVN002ZV fan failure in Daya Bay Nuclear Power Station

The present emphasis on inherent safety for liquid-metal reactor designs has resulted in a need to represent the various reactivity feedback mechanisms as accurately as possible. The dominant negative reactivity feedback has been found to result from radial expansion of the core for most postulated anticipated transient without scram events. For this reasons, a more detailed model for calculating the reactivity feedback from radial core expansion, including subassembly bowing has been recently developed for use with the SASSYS/SAS4A code system. The purpose of this summary is to present an extension to the model so that it is more suitable for handling a core restraint design as used in the Fast Flux Test Facility (FFTF), and to compare the SASSYS/SAS4A results using this model to the empirical correlation presently being used to account for radial core expansion reactivity feedback in the FFTF.

1987-11-15

103

Flywheel energy storage construction; Vliegwielenergieopslaginrichting

Extensive investigations and detailed analysis of the failure reason of 9DVN002ZV fan in Daya Bay Nuclear Power Station showed that the fan destroy was caused by the failure of non-drive end bear. The direct cause of this bearing' failure was its improper assembly caused by improper maintenance procedure, and the root cause was too small internal radial clearance after mounting. The factor affecting bearing internal radial clearance, the relationship between clearance and operating life time and fan failure process were discussed. (authors)

2005-09-01

104

Dynamics of the Edge Transport Barrier at Plasma Biasing on the Castor Tokamak

The title invention aims at a vibrations-free running of the flywheel to realize a high speed (18,000-20,000 revolutions per minute). The flywheel consists of a case which holds a central stator, a rotor and two ball-bearings. The ball-bearings have a first radial stiffness and are placed in between the rotor and an upper, respectively lower support bearer. The support bearers are supported on the stator with a second radial stiffness. The top and the bottom of the stator is connected to the case by means of a support with a third stiffness. 6 figs.

1995-06-01

105

Comparative radial distribution analysis of the short range order in metallic glass Al_0_._9_1La_0_._0_9 and crystalline Al_1_1La_3

A clear and reproducible transition to a regime with an improved particle confinement is routinely observed on the CASTOR tokamak, if the biasing electrode is inserted deep enough into the plasma (r/a?0.5) and biased up to +250 V. The steepening of the radial profiles of the plasma density and potential demonstrate the formation of a transport barrier just inside the last closed flux surface. Fast relaxations of the edge plasma parameters, with a frequency of about 10 kHz, are observed when the average radial electric field within the barrier prevails values of about 20 kV/m. A detailed analysis of the spatial-temporal behaviour of these relaxations is presented.

2006-01-01

106

Analysis of Longitudinal Space Charge Effects With Radial Dependence

XAFS data of metallic glass Al_0_._9_1La_0_._0_9 and a crystalline phase Al_1_1La_3 formed by annealing of the glass were measured at the La L_3 edge at T=12 K and analyzed using the radial distribution function method. The shortest La-Al distance appeared to be distinctively smaller within the glass than in the crystal. This difference decreases the disparity in size of La and Al in the alloy, allowing their mixing in the glassy state. ((orig.)).

1994-09-02

107

A peculiar distribution of radial velocities of faint radio-galaxies with 13.0<=msub(corr)<=15.5

Longitudinal space charge (LSC) force can be a main effect driving the microbunching instability in the linac for an x-ray free-electron laser (FEL). In this paper, the LSC-induced beam modulation is studied using an integral equation approach that takes into account the transverse (radial) variation of LSC field for both the coasting beam limit and bunched beam. Changes of beam energy and the transverse beam size can be also incorporated. We discuss the validity of this approach and compare it with other analyses as well as numerical simulations.

2005-09-30

108

A note on the pressure field within an outward moving free annulus

A sample of 41 radio-galaxies with 13.0<=msub(corr)<=15.5 has been analyzed to test the angular redshift anisotropy discovered on Sc I galaxies by Rubin, Rubin and Ford (1973). The sample does not present their anisotropy but contains an even more curious distribution of radial velocities which suggests that the Rubin-Ford effect results from an anomalous redshift of light when it travels through clusters of galaxies. (Auth.).

109

104 MeV alpha particle scattering from /sup 90/,/sup 92/Zr

The outward radial expansion of a free liquid annulus is a common problem of both earlier and current ICF blanket design. Whether the annulus fractures or not depends on the internal pressure and surface stability. In this paper a model based on incompressible cylindrically symmetric flow is used to get a theoretical solution similar to that of the Rayleigh's solution for bubble dynamics. The pressure inside the annulus is found positive all time but the peak is lowering during the expansion. Besides, both surfaces are Taylor stable during such motion. Thus, it is concluded that an annulus in outward radial motion will not cavitate or breakup.

1990-01-01

110

104 MeV alpha particle scattering from "9"0,"9"2Zr

Differential cross sections have been measured for the elastic and inelastic scattering of 104 MeV alpha-particles from /sup 90/,/sup 92/Zr. The experimental data are analyzed in terms of coupled channels on the basis of a flexible anharmonic vibrator model and using different parametrizations of the radial shape of the extended optical potential. The results favour the squared Saxon-Woods type for the real part. Additionally to a radial momentum analysis of the real potentials a semimicroscopic folding model has been invoked for extracting isoscalar quadrupole and hexadecapole transition rates.

1982-05-01

111

Thermal decomposition of gamma irradiated proteins by thermal analysis methods

Differential cross sections have been measured for the elastic and inelastic scattering of 104 MeV alpha-particles from "9"0,"9"2Zr. The experimental data are analyzed in terms of coupled channels on the basis of a flexible anharmonic vibrator model and using different parametrizations of the radial shape of the extended optical potential. The results favour the squared Saxon-Woods type for the real part. Additionally to a radial momentum analysis of the real potentials a semimicroscopic folding model has been invoked for extracting isoscalar quadrupole and hexadecapole transition rates. (orig.).

112

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator

Short communication. 2 figs.

1996-12-31

113

Temporal Fluctuations of Fluorescence Energy Transfer between TwoDyes Conjugated to a Single Protein

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

2005-01-01

114

Structure and Function of NtCDPK1, a Calcium-dependent Protein Kinase in Tobccco

No abstract prepared.

1999-01-04

115

Protein profile changes in platelet concentrates according to storage and leukoreduction- analysis using proteomics technology

Full Text Available

2000-05-01

116

Moessbauer studies of the molybdenum-iron-protein in the nitrogenase of Xanthobacter autotrophicus GZ 29. Moessbaueruntersuchungen am Molybdaen-Eisen-Protein der Nitrogenase von Xanthobacter Autotrophicus GZ 29

Full Text Available

2008-02-01

117

METHOD FOR SYNTHESIZING TAURINE FROM PROTEIN-CONTAINING MATERIAL BY USING HIGH-TEMPERATURE HIGH-PRESSURE WATER

Biological cells of the nitrogen fixating bacteria Xanthobacter autotrophicus GZ 29 have been cultivated with 57-Fe, and from the nitrogenase of these cells the 57-Fe marked molybdenum-iron-protein is extracted. The Moessbauer spectra of this enzyme show that the structure of the iron cluster is similar to the structure in the molybdenum-iron-proteins of Azotobacter vinelandii, Klebsiella pneumoniae or Clostridium pasteurianum.

1983-05-01

118

J-STORE (Japan)

Full Text Available

2003-08-28

119

High Throughput Screening on Angiogenesis Inhibitor and Promoter of Medicinal Plants using a Protein Microarray Chip

Expression of microtubule associated protein of low molecular weight (TAU) in mouse brain development

Full Text Available

2007-04-01

120

Effect of the Toluene Administration on the Liver Damage in Rats Fed Low or Standard Protein Diet

Published in summary form only.

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121

Effect of nitric oxide on endoplasmic reticulum calcium homeostasis, protein synthesis and energy metabolism

Full Text Available

1996-12-01

122

DEFF Research Database (Denmark)

ATPase, endoplasmic reticulum, calcium, ischaemia

2000-01-01

123

Effect of Dietary Protein on the Changes of Lipoprotein Fractions in Carbon Tetrachloride-Treated Rats

Effect of Dietary Energy, Protein on Growth and Blood Composition of Cross Bred Chicks

Full Text Available

1993-04-01

124

Effect of Angiotensin-I Converting Enzyme Inhibitory from Hydrolysate of Soybean Protein Isolate

Full Text Available

2008-09-01

125

Dyeability and Functionality of Pine Needles Extract (Part II) -Dyeing Properties of Protein Fiber-

Full Text Available

2010-01-01

126

Deposition of Matrix and Crystalloid Storage Proteins during Protein Body Development in the Endosperm of Ricinus communis L. cv. Hale Seeds ¹

Full Text Available

2011-04-01

127

Comparative Study on Protein and Amino Acid Composition of Wild and Cultured Eel

Protein bodies within the endosperm of castor bean (Ricinus communis L. cv. Hale) seeds arise from numerous small vacuoles which progressively become filled with storage protein, of...Full Text Available

1982-06-01

128

Biochemical characterization of Alanine racemase- a spore protein produced by Bacillus anthracis

Full Text Available

1986-01-01

129

Bilateral retinal dysplasia and secondary glaucoma associated with homozygous protein C deficiency.

Full Text Available

2009-01-01

130

Effect of host nutrition on immunity and local immune response of rabbits to Obeliscoides cuniculi

Full Text Available

2005-06-01

131

Bacteriophage P22 capsid size determination: Roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus

In a series of experiments carried out on young and adult rabbits the effect of isocaloric low protein diets containing 4% or 8% protein compared with a diet containing 21% protein on Obeliscoides cuniculi infection was studied. The pathogenesis, resistance and local immunity were assessed after single infections with 10,000 larvae or reinfection with 5000 larvae. Live weight gain was reduced in young and adult rabbits fed the low protein diets, but the establishment of parasites was not substantially influenced by protein deprivation. However, development of worms in the histotrophic phase and parasite fecundity were impaired in association with the low protein diet. Moreover, mild anaemia as well as changes in the mucosal immune response as a result of infection were related to the level of dietary protein. (author). 30 refs, 6 figs, 5 ...

1987-05-11

132

British Library Electronic Table of Contents (United Kingdom)

Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a sing...

2011-01-01

133

Identification of proteins important for tetracycline (TC) binding to ribosomes by single protein omission reconstitution (SPORE) experiments

Protein expression in white spot syndrome virus infected Penaeus monodon fabricius.

TC inhibits protein synthesis in E. coli by interfering with aminoacyl-tRNA binding to the ribosomal A site, and there is strong evidence that such inhibition results from TC binding to a high affinity site on the 30S subunit. The SPORE approach has been used to define those 30S proteins that are potentially important for high affinity TC binding, measured as the (/sup 3/H)-TC co-sedimenting with the reconstitution particle through a sucrose density gradient. Reverse phase-HPLC has been used both to prepare ribosomal proteins and to analyze the protein content of reconstituted particles. The results obtained so far show that protein S7, as well as some proteins linked to S7 in the 30S assembly map, are important for TC binding, whereas other ribosomal proteins are not. These results are in very good accord with their earlier photoaffinity ...

1987-05-01

134

T-2 mycotoxin inhibits mitochondrial protein synthesis

White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart ...

2005-06-01

135

Phosphorylation of proteins in Clostridium thermohydrosulfuricum

The authors investigated the effect of T-2 toxin on rat liver mitochondrial protein synthesis. Isolated rat liver mitochondria were supplemented with an S-100 supernatant from rat liver and an external ATP-generating system. An in-vitro assay employing cycloheximide, and inhibitor of cytoplasmic protein synthesis, and chloramphenicol, and inhibitor of mitochondrial protein synthesis, to distinguish mitochondrial protein synthesis from the cytoplasmic process. Amino acid incorporation into mitochondria was dependent on the concentration of mitochondria and was inhibited by chloramphenicol. The rate of uptake of tritium leucine into mitochondrial protein was unaffected by the addition of T-2 toxin and was not a rate-limiting step in incorporation. However, 0.02 micrograms/ml of T-2 toxin decreased the rate of protein synthesis inhibition correlated with the amount ...

1988-01-01

136

Affinity purification of sequence-specific DNA binding proteins.

Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd ...

1986-02-01

137

The Sorcerer II Global Ocean Sampling Expedition: Expanding theUniverse of Protein Families

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography ...

1986-08-01

138

Uniform and Residue-specific {sup 15}N-labeling of Proteins on a Highly Deuterated Background

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

2006-03-23

139

Proteomic analysis of sericin in Bombyx mori cocoons

A general method for stable-isotope labeling of large proteins is introduced and applied for studies of the E. coli GroE chaperone proteins by solution NMR. In addition to enabling the residue-specific {sup 15}N-labeling of proteins on a highly deuterated background, it is also an efficient approach for uniform labeling. The method meets the requirements of high-level deuteration, minimal cross-labeling and high protein yield, which are crucial for NMR studies of structures with sizes above 150 kDa. The results obtained with the new protocol are compared to other strategies for protein labeling, and evaluated with regard to the influence of external factors on the resulting isotope labeling patterns. Applications with the GroE system show that these strategies are efficient tools for studies of structure, dynamics and intermolecular interactions in large supramolecular complexes, ...

2004-07-15

140

British Library Electronic Table of Contents (United Kingdom)

Cocoon sericin plays an important role in the reeling of silk and serves as a valuable biomaterial in the field of biomedicine, skincare, and food industries; however, knowledge about cocoon sericin proteins has been limited. For a comprehensive study on sericin, cocoons of eight varieties of silkworm of different geographic origin and with varied cocoon color were analyzed utilizing proteomics and bioinformatics approaches. The electrophoresis pattern demonstrated some common protein bands for all silkworm varieties and distinctive protein bands for some of those examined in the present study. The Ser2 protein, a new Ser3 protein, and four other novel sericin proteins were identified in cocoons for the first time. Products of both Ser1 and Ser3 genes appear to be ubiquitous in the cocoon ...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

Modeling protein synthesis from a physicist's perspective: a toy model

Mechanism of Dephosphorylation of the SR Protein ASF/SF2 by Protein Phosphatase 1

Proteins are polymers of amino acids. These macromolecules are synthesized by intracellular machines called {\\it ribosome}. Although, traditionally, the experimental investigation of protein synthesis has been an active area of research in molecular cell biology, important quantitative models of this phenomenon have been reported mostly in the research journals devoted to statistical physics and related interdisciplinary topics. From the perspective of a physicist, protein synthesis is a phenomenon of {\\it classical transport of interacting ribosomes on a messenger RNA (mRNA) template} that dictates the sequence of the amino acids on the protein. Here we bring this frontier area of contemporary research into the classroom by appropriate simplification of the models and methods. In particular, we develope a simple toy model and analyze it by some elementary techniques of non-equilibrium statistical ...

2007-01-01

142

British Library Electronic Table of Contents (United Kingdom)

SR proteins are essential splicing factors whose function is controlled by multi-site phosphorylation of a C-terminal domain rich in arginine-serine repeats (RS domain). The protein kinase SRPK1 has been shown to polyphosphorylate the N-terminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear entry of this splicing factor and engagement in splicing function. Later, dephosphorylation is required for maturation of the spliceosome and other RNA processing steps. While phosphates are attached to RS1 in a sequential manner by SRPK1, little is known about how they are removed. To investigate factors that control dephosphorylation, we monitored region-specific mapping of phosphorylation sites in ASF/SF2 as a function of the protein phosphatase PP1. W...

2010-01-01

143

Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

British Library Electronic Table of Contents (United Kingdom)

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50??g/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30?min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

2011-01-01

144

Spacetime constraints on accreting black holes

Regular frequency patterns in the classical delta Scuti star HD 144277 observed by the MOST satellite

We study the spin dependence of accretion onto rotating Kerr black holes using analytic techniques. In its linear regime, angular momentum transport in MHD turbulent accretion flow involves the generation of radial magnetic field connecting plasma in a differentially rotating flow. We take a first principles approach, highlighting the constraint that limits the generation and amplification of radial magnetic fields, stemming from the transfer of energy from mechanical to magnetic form. Because the energy transferred in magnetic form is ultimately constrained by gravitational potential energy or Killing energy, the spin dependence of the latter allows us to derive spin-dependent constraints on the success of the accreting plasma to expel its angular momentum. We find an inverse relationship between this ability and black hole spin. If this radial magnetic field generation forms the basis for angular momentum transfer in ...

2009-06-15

145

Radial keratoneuritis as a presenting sign in acanthamoeba keratitis.

We present high-precision time-series photometry of the classical delta Scuti star HD 144277 obtained with the MOST (Microvariability and Oscillations of STars) satellite in two consecutive years. The observed regular frequency patterns are investigated asteroseismologically. HD 144277 is a hot A-type star that is located on the blue border of the classical instability strip. While we mostly observe low radial order modes in classical delta Scuti stars, HD 144277 presents a different case. Its high observed frequencies, i.e., between 59.9c/d (693.9 microHz) and 71.1c/d (822.8microHz), suggest higher radial orders. We examine the progression of the regular frequency spacings from the low radial order to the asymptotic frequency region. Frequency analysis was performed using Period04 and SigSpec. The results from the MOST observing runs in 2009 and 2010 were compared to each other. The resulting frequencies were submitted to ...

2011-01-01

146

On the origin of the Trojan asteroids Effects of Jupiter's mass accretion and radial migration

The visual outcomes of Acanthamoeba keratitis, a rare cause of corneal infection, can be devastating. This paper reports two contact lens wearers with severe pain and photophobia who presented to the emergency room. Biomicroscopy revealed radial keratoneuritis in both individuals. Tissue culture on a nonnutrient agar plate with Escherichia coli overlay resulted in a heavy growth of Acanthamoeba. The inpatient treatment included 0.02% polyhexamethylene biguanide, chlorhexidine, neomycin/polymyxin B/bacitracin (Neosporin), and oral fluconazole, which successfully controlled the corneal infection and improvement in the best corrected visual acuity in both patients. Infection did not recur during the 12-month follow-up period. Acanthamoeba keratitis can present as radial keratoneuritis, mimicking other common corneal infections resulting in diagnostic and treatment delays. Early diagnosis and prudent treatment of Acanthamoeba keratitis are the keys ...

2011-07-01

147

Manufacturing of small scale W monoblock mockups by hot radial pressing

We present analytic and numerical results which illustrate the effects of Jupiter's accretion of nebular gas and the planet's radial migration on its Trojan companions. Initially, we approximate the system by the planar circular restricted three-body problem and assume small Trojan libration amplitudes. Employing an adiabatic invariant calculation, we show that Jupiter's thirty-fold growth from a $10 M_\\oplus$ core to its present mass causes the libration amplitudes of Trojan asteroids to shrink by a factor of about 2.5 to $\\sim 40%$ of their original size. The calculation also shows that Jupiter's radial migration has comparatively little effect on the Trojans; inward migration from 6.2 to 5.2 AU causes an increase in Trojan libration amplitudes of $\\sim4%$. In each case, the area enclosed by small tadpole orbits, if made dimensionless by using Jupiter's semimajor axis, is approximately conserved. Similar adiabatic invariant calculations ...

2000-01-01

148

Experimental investigation of the thermal contact resistance of a space-use deep groove ball bearing. Uchuyo fukamizo tamajikuukenai no sesshoku netsu teiko ni kansuru jikkenteki kento

In the frame of the European Technology R and D programme for International thermonuclear experimental reactor (ITER) and in the area of high heat flux plasma facing components (HHFC), representative small-scale mock-ups were manufactured and tested to compare different concepts and joining technologies (i.e. active brazing, hot isostatic pressing (HIPping), diffusion bonding, etc.). On the basis of the results obtained by thermal fatigue tests, the monoblock concept resulted to be the most robust one, particularly when the HIPping manufacturing technology is used. Within this programme, ENEA developed an alternative technique for manufacturing plasma-facing components with a monoblock geometry of the ITER machine. The basic idea of this technique, named hot radial pressing (HRP), is to perform a radial diffusion bonding between the cooling tube and the armour tile by pressurising the internal tube only and by keeping the process parameters ...

2003-09-01

149

Application of calibration-free laser-induced breakdown spectroscopy to radially resolved spectra from a copper-based alloy laser-induced plasma

The thermal contact resistance between balls and inner and outer rings of thermally steady space-use deep groove ball bearings was studied experimentally to verify the validity of the previously proposed calculation method for the resistance. The 440C stainless steel single row bearing was used for measuring its steady state temperature distributions in a vacuum environment under axial, radial and combined load conditions. As a result, the experimental results of the resistance well agreed with calculated ones under each load condition resulting in the sufficient validity at nearly the same surface roughness. It was necessary to apply a static equivalent radial load to calculation under the combined load that a contact angle is larger than the angle of resultant force dependent on both load components. In experiments under only radial load, measurement of outer ring temperatures was necessary at some points with no contact ...

1992-12-05

150

Application of calibration-free laser-induced breakdown spectroscopy to radially resolved spectra from a copper-based alloy laser-induced plasma

In this work, the Calibration-Free approach for Laser-Induced Breakdown Spectroscopy (CF-LIBS) was applied for the first time to radially resolved spectra emitted by a laser-induced plasma. The radial profiles of plasma temperature and electron number density were used to calculate the local relative concentration of the elements of interest. We analyzed a set of profiles of the local spectral emission coefficient obtained previously by means of spatial deconvolution of the spectra from a copper-based alloy (Cu 93, Fe 5, Mn 1, Ni 1 wt.%) laser-induced plasma. A spatially integrated spectrum of the same plasma was also analyzed for comparison purpose. The relative abundance of the minor components Fe, Mn and Ni was calculated. The results obtained from the central region of the plasma were closer to the nominal concentrations than those obtained from the spatially integrated spectrum. However, an increasing deviation was observed towards the ...

2009-07-15

151

RADIAL DISTRIBUTION OF STARS, GAS AND DUST IN SINGS GALAXIES. I. SURFACE PHOTOMETRY AND MORPHOLOGY

In this work, the Calibration-Free approach for Laser-Induced Breakdown Spectroscopy (CF-LIBS) was applied for the first time to radially resolved spectra emitted by a laser-induced plasma. The radial profiles of plasma temperature and electron number density were used to calculate the local relative concentration of the elements of interest. We analyzed a set of profiles of the local spectral emission coefficient obtained previously by means of spatial deconvolution of the spectra from a copper-based alloy (Cu 93, Fe 5, Mn 1, Ni 1 wt.%) laser-induced plasma. A spatially integrated spectrum of the same plasma was also analyzed for comparison purpose. The relative abundance of the minor components Fe, Mn and Ni was calculated. The results obtained from the central region of the plasma were closer to the nominal concentrations than those obtained from the spatially integrated spectrum. However, an increasing deviation was observed towards the ...

2009-07-01

152

Wind turbulence estimates in a valley by coherent Doppler lidar

We present ultraviolet through far-infrared (FIR) surface brightness profiles for the 75 galaxies in the Spitzer Infrared Nearby Galaxies Survey (SINGS). The imagery used to measure the profiles includes Galaxy Evolution Explorer UV data, optical images from Kitt Peak National Observatory, Cerro Tololo Inter-American Observatory, and Sloan Digital Sky Survey, near-IR data from Two Micron All Sky Survey, and mid- and FIR images from Spitzer. Along with the radial profiles, we also provide multi-wavelength asymptotic magnitudes and several nonparametric indicators of galaxy morphology: the concentration index (C 42), the asymmetry (A), the Gini coefficient (G), and the normalized second-order moment of the brightest 20% of the galaxy's flux (M-bar20). In this paper, the first of a series, we describe the technical aspects regarding the surface photometry, and present a basic analysis of the global and structural properties of the SINGS galaxies at different ...

2009-10-01

153

British Library Electronic Table of Contents (United Kingdom)

Abstract In this paper, the effect of several turbulence parameters during various flow conditions in Owens Valley, educed from coherent Doppler lidar data have been studied. Radial velocity structure functions are processed to estimate the turbulent kinetic energy (TKE) dissipation rate, integral length scale and velocity variance, assuming a theoretical model for isotropic wind fields. Corrections for turbulence measurements have been considered to address the complications due to inherent volumetric averaging of radial velocity over each range gate, noise of the lidar data, and the assumptions required to estimate effects of smaller scales of motion on turbulence quantities. Using data from the Terrain-induced Rotor Experiment (T-REX) in April-May 2006, vertical profiles of wind and tur...

2011-01-01

154

The radial distribution of the neutron field in the core of Dalat reactor

Rotary drill bit with rotary cutter

Determining the radial distribution of the thermal neutron field in the core of the Dalat reactor was done by the Cu foil activation method. The measured data were fitted by the least square method to determine some physical parameters of the reactor, as follows: 1. Laplacian: B_r"2 = (84.6 +- 5.5)10_-_4/,cm"2. 2. The effective radius: R_e_f_f = (27.6 +- 1.0)cm. 3. The extrapolation distance: #lambda#_r = (8.7 +- 1.0)cm. 4. The unequal coefficient of the effective multiplication: k_r = 1.77 +- 0.11. (author). 3 refs., 4 figs., 1 tab.

1992-01-01

155

Radial distribution functions. 5

A rotary drill bit having a drill bit body and at least one trunnion projecting from the drill bit body and a rotary cutter supported on at least one radial roller bearing on the trunnion. The rolling elements of the bearing are guided on at least one axial end facing the drill bit body in an outer bearing race groove incorporated in the bore of the rotary cutter. The inner bearing race groove is formed on the trunnion for the rolling elements of the radial roller bearing. At least one filling opening is provided which extends through the drill bit body and trunnion and is essentially axially oriented having one terminal end adjacent the inner bearing race groove and at least one filler piece for sealing the opening.

1981-03-17

156

Pseudospin symmetry and the relativistic harmonic oscillator

The noncrystalline compounds MoS_3, WS_3 and MoSe_3 were studied by X-ray diffraction. From the diffuse intensities radial distribution functions were computed and interpreted in terms of pair distribution functions. The substances are built up by microcrystallites which contain three metal atoms and are bridged statistically to each another. The shortest metal chalcogen distances d(MoS) approximately d(WS) = 2.40 +- 0.05 A and d(MoSe) = 2.50 +- 0.05 A are significantly longer than in MS- or MSe compounds with a d"0 configuration of the metal respectively and metal-metal distances within the microcrystallites are less than 3 A. (author).

1977-01-01

157

On the radial distribution of gamma rays in the outer galaxy

A generalized relativistic harmonic oscillator for spin 1/2 particles is studied. The Dirac Hamiltonian contains a scalar, $S$, and a vector, $V$, quadratic potentials in the radial coordinate, as well as a tensor potential, $U$, linear in $r$. Setting either or both combinations $\\Sigma=S+V$ and $% \\Delta=V-S$ to zero, analytical solutions for bound states of the corresponding Dirac equations are found. The eigenenergies and wave functions are presented and particular cases are discussed, devoting a special attention to the non-relativistic limit and the case $\\Sigma=0$, for which pseudospin symmetry is exact. We also show that the case $U=\\Delta=0$ is the most natural generalization of the non-relativistic harmonic oscillator. The radial node structure of the Dirac spinor is studied for several combinations of harmonic oscillator potentials, and that study allows us to explain why nuclear intruder levels cannot be described in the ...

2004-01-01

158

Liquid crystal films on curved surfaces: An entropic sampling study

The authors describe a new method which makes it possible to determine the radial distribution of the diffuse component of galactic gamma rays outside the solar circle. They use the observation that a good correlation exists between gamma-ray intensities and total column densities of the local interstellar gas and that the fractional column density of H_2<0.1 HI outside the solar circle. Thus the gamma-ray intensities are shown to be proportional to N(HI). The authors use the kinematics of the HI to determine the distances from which various fractions of the emission originate in the second and third galactic quadrants. Preliminary results of our analysis show that a significant flux of gamma rays originates from distances as large as 18 kpc from the galactic centre. (Auth.).

1982-08-04

159

Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis

The confining effect of a spherical substrate inducing anchoring (normal to the surface) of rod-like liquid crystal molecules contained in a thin film spread over it has been investigated with regard to possible changes in the nature of the isotropic-to-nematic phase transition as the sample is cooled. The focus of these Monte Carlo simulations is to study the competing effects of the homeotropic anchoring due to the surface inducing orientational ordering in the radial direction and the inherent uniaxial order promoted by the intermolecular interactions. By adopting entropic sampling procedure, we could investigate this transition with a high temperature precision, and we studied the effect of the surface anchoring strength on the phase diagram for a specifically chosen geometry. We find that there is a threshold anchoring strength of the surface below which uniaxial nematic phase results, and above which the isotropic fluid cools to a ...

2010-01-01

160

Werner syndrome protein interacts functionally with translesion DNA polymerases

Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients with WAS exhibit both immunodeficiency and a marked susceptibility to systemic autoimmunity. We investigated...Full Text Available

2007-02-01

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161

Water Molecule Contributions to Proton Spin-Lattice Relaxation in Rotationally Immobilized Proteins

Werner syndrome (WS) is characterized by premature onset of age-associated disorders and predisposition to cancer. The WS protein, WRN, encodes 3′ → 5′ DNA helicase and 3′...Full Text Available

2007-06-19

162

Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space

Spin-lattice relaxation rates of protein and water protons in dry and hydrated immobilized bovine serum albumin were measured in the range of ¹H Larmor frequency from 10 kHz to 30...Full Text Available

2009-07-01

163

Use of synthetic oligoribonucleotides to probe RNA-protein interactions in the MS2 translational operator complex.

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

2010-12-01

164

Use of Dimethyl Suberimidate, a Cross-Linking Reagent, in Studying the Subunit Structure of Oligomeric Proteins^*

Synthetic oligoribonucleotides have been used to probe the interaction of MS2 coat protein with the translational operator of the MS2 replicase gene. We have investigated the possible formation of a...Full Text Available

1990-06-25

165

Use of DNA ladders for reproducible protein fractionation by SDS-PAGE for quantitative proteomics

Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase...Full Text Available

1970-07-01

166

The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription

In proteomics, one-dimensional (1D) SDS-PAGE is widely used for protein fractionation prior to mass spectrometric analysis to enhance dynamic range of analysis and to improve identification...Full Text Available

2008-02-01

167

The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei.

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

2005-01-01

168

The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis

We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter...Full Text Available

1994-10-11

169

The discovery of signal transduction by G proteins. A personal account and an overview of the initial findings and contributions that led to our present understanding

Altered structure, and hence function, of cellular macromolecules caused by oxidation can contribute to loss of physiological function with age. Here, we tested whether the lifespan of bats, which generally...Full Text Available

2009-07-01

170

The Effects of Aspirin-Like Drugs on the Nutritional Status of Pregnant Rats and Offspring

The realization that there existed a G-protein coupled signal transduction mechanism developed gradually and was initially the result of an ill fated quest for uncovering the mechanism of action...Full Text Available

2007-04-01

171

Synthesis and Phosphorylation of Maize Acidic Ribosomal Proteins1

Previous studies have shown that salicylates and protein-calorie malnutrition independently compromise maturation and growth of infants. In the present study, pregnant rats were fed normal-and low-protein...Full Text Available

1988-05-01

172

Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid

The objective of this research was to determine the role of acidic ribosomal protein (ARP) phosphorylation in translation. Ribosomes (Rbs) from germinated maize (Zea mays L.) axes had...Full Text Available

1998-01-01

173

Stimulation of Phosphatidylethanolamine Exchange by Castor Bean Cytosol Proteins ¹

Surfactant protein A (SP-A), first identified as a component of the lung surfactant system, is now recognized to be an important contributor to host defence mechanisms. SP-A can facilitate phagocytosis...Full Text Available

2004-01-01

174

Simulation of the electron-paramagnetic-resonance spectrum of the iron-protein of nitrogenase. A prediction of the existence of a second paramagnetic centre.

Cytosol proteins prepared from castor bean endosperm (4-day-old) seedlings stimulate the exchange of [³H]phosphatidylethanolamine between liposomes and mitochondria. The acceleration of the...Full Text Available

1980-02-01

175

Seroprevalence of HIV-1 and HIV-2 infection among children diagnosed with protein-calorie malnutrition in Nigeria.

The e.p.r. spectra of the Fe-proteins of nitrogenase from all sources studied have unusual features in that they have very anisotropic linewidths and low integrated intensities. These characteristics...Full Text Available

1978-12-01

176

Role in Virulence of a Brucella abortus Protein Exhibiting Lectin-Like Activity

Excessive weight loss due to protein calorie malnutrition (PCM) is a significant problem in Nigerian children. This syndrome may be difficult to differentiate from the wasting disease caused by human...Full Text Available

1993-04-01

177

Risk factors for thrombosis in lupus patients.

Brucella abortus is a facultative, intracellular zoonotic pathogen which can cause undulant fever in humans and abortions in cattle. A 14-kDa protein of B. abortus...Full Text Available

2006-01-01

178

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Lupus anticoagulant, concentrations of anticardiolipin antibodies, antithrombin III, plasminogen, (free) protein S, protein C, prothrombin, platelet counts, and bleeding times were determined in 74...Full Text Available

1989-11-01

179

Proteomic approach with LCMS-IT-TOF identified an increase of Rab33B after transient focal cerebral ischemia in mice

Carbenoxolone (CBX) is a semisynthetic derivative of the licorice root substance glycyrrhizinic acid and has been previously reported to induce only heat shock protein 70 [Hsp70, HSPA1A (the systematic...Full Text Available

2009-09-01

180

Predicting Protein Post-translational Modifications Using Meta-analysis of Proteome Scale Data Sets*S?

BackgroundSeveral proteins are known to be markedly expressed in the brain during cerebral ischemia; however, the changes in protein profiles within the ischemic brain after an ischemic...Full Text Available

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181

Plasma protein binding of phenytoin in 100 epileptic patients.

Protein post-translational modifications are an important biological regulatory mechanism, and the rate of their discovery using high throughput techniques is rapidly increasingly. To make use of this...Full Text Available

2009-02-01

182

Photophysics and optical switching in green fluorescent protein mutants

The plasma protein binding of phenytoin was investigated in 100 epileptic patients, using equilibrium dialysis at 37 degrees C. The unbound fractions of phenytoin in plasma formed a skewed distribution,...Full Text Available

1982-08-01

183

Pharmacokinetics and protein binding of ceftriaxone during pregnancy.

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not...Full Text Available

2000-03-28

184

Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

The purpose of the present work was to study the pharmacokinetics and the protein binding (free fraction of the drug) of ceftriaxone (CTX) during pregnancy. Nine pregnant women (ages, 20 to 34 years)...Full Text Available

1993-01-01

185

Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

186

Multiple-Bond Kinetics from Single-Molecule Pulling Experiments: Evidence for Multiple NCAM Bonds

BackgroundZhx1 to 3 (zinc-fingers and homeoboxes) form a set of paralogous genes encoding multi-domain proteins. ZHX proteins consist of two zinc fingers followed...Full Text Available

187

Molecular Identification and Expression Analysis of Filaggrin-2, a Member of the S100 Fused-Type Protein Family

The kinetic parameters of single bonds between neural cell adhesion molecules were determined from atomic force microscope measurements of the forced dissociation of the homophilic protein-protein bonds....Full Text Available

2005-11-01

188

Molecular Architecture of the Centriole Proteome: The Conserved WD40 Domain Protein POC1 Is Required for Centriole Duplication and Length Control

Genes of the S100 fused-type protein (SFTP) family are clustered within the epidermal differentiation complex and encode essential components that maintain epithelial homeostasis and barrier functions....Full Text Available

189

Metal Ions-Stimulated Iron Oxidation in Hydroxylases Facilitates Stabilization of HIF-1? Protein

Centrioles are intriguing cylindrical organelles composed of triplet microtubules. Proteomic data suggest that a large number of proteins besides tubulin are necessary for the formation and maintenance...Full Text Available

2009-02-15

190

Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

The exposure of cells to several metal ions stabilizes HIF-1α protein. However, the molecular mechanisms are not completely understood. They may involve inhibition of hydroxylation by either...Full Text Available

2009-02-01

191

Measurements of accurate x-ray scattering data of protein solutions using small stationary sample cells

Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing...Full Text Available

2010-01-01

192

MFR, a Putative Receptor Mediating the Fusion of Macrophages

In this paper, we report a method of precise in situ x-ray scattering measurements on protein solutions using small stationary sample cells. Although reduction in the radiation damage...Full Text Available

2009-01-01

193

M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

We had previously identified a macrophage surface protein whose expression is highly induced, transient, and specific, as it is restricted to actively fusing macrophages in vitro and in vivo. This protein...Full Text Available

1998-11-01

194

Los Alamos sequence analysis package for nucleic acids and proteins.

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

1989-09-01

195

Lipoprotein lipase deficiency is associated with elevated acylation stimulating protein plasma levels

An interactive system for computer analysis of nucleic acid and protein sequences has been developed for the Los Alamos DNA Sequence Database. It provides a convenient way to search or verify various...Full Text Available

1982-01-11

196

Linking Gene Expression and Functional Network Data in Human Heart Failure

Acylation stimulating protein (ASP, C3adesArg) is an adipose tissue derived hormone that stimulates triglyceride (TG) synthesis. ASP stimulates lipoprotein lipase (LPL) activity by relieving feedback...Full Text Available

2009-06-01

197

Lifespan extension by increased expression of the Drosophila homologue of the IGFBP7 tumour suppressor

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

198

Lectin Binding of the Major Polar Tube Protein (PTP1) and its Role in Invasion

Mammals possess multiple insulin-like growth factor (IGF) binding proteins (IGFBPs), and related proteins, that modulate the activity of insulin/IGF signalling (IIS), a conserved neuroendocrine signalling...Full Text Available

2011-02-01

199

Large-scale Analysis of Thermo-stable, Mammalian Proteins Provides Insights into the Intrinsically Disordered Proteome

... 2003)050[0600:LBOTMP]2.0.CO;2 Lectin Binding of the Major Polar Tube Protein (PTP1) ... PTP1. We, therefore, undertook a study ...

200

Lack of association between polymorphisms in C4b-binding protein and atypical haemolytic uraemic syndrome in the Spanish population

Intrinsically disordered proteins are predicted to be highly abundant and play broad biological roles in eukaryotic cells. In particular, by virtue of their structural malleability and propensity...Full Text Available

2009-01-01

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201

Isolation and Characterization of a Novel Nuclear Protein from Pollen Mother Cells of Lily

Dysregulation of the alternative pathway of complement activation, caused by mutations or polymorphisms in the genes encoding factor H, membrane co-factor protein, factor I or factor B, is associated...Full Text Available

2009-01-01

202

Involvement of AMP-activated-protein-kinase (AMPK) in neuronal amyloidogenesis

Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G₂...Full Text Available

1990-11-01

203

Investigation of Early Protein Changes in the Urinary Bladder Following Partial Bladder Outlet Obstruction by Proteomic Approach

AMP-activated-protein-kinase (AMPK) is a key sensor and regulator of cellular and whole-body energy metabolism and plays a key role in regulation of lipid metabolism. Since lipid metabolism...Full Text Available

2010-09-03

204

Intracellular delivery of an antisense?oligonucleotide via endocytosis of a G protein-coupled receptor

We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO)....Full Text Available

2005-12-01

205

Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress

Gastrin-releasing peptide receptor (GRPR), a member of the G protein-coupled receptor superfamily, has been utilized for receptor-mediated targeting of imaging and therapeutic agents; here we extend...Full Text Available

2010-10-01

206

Insulin-induced decrease in protein phosphorylation in rat adipocytes not explained by decreased A-kinase activity

BackgroundThe VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of...Full Text Available

207

Insertion in the mRNA of a metachromatic leukodystrophy patient with sphingolipid activator protein-1 deficiency.

In isolated rat adipocytes, insulin inhibits lipolysis to a greater extent than would be predicted by the decrease in (-/+)cAMP activity ratio of cAMP-dependent protein kinase (A-kinase), from which it was speculated that insulin promotes the dephosphorylation of hormone-sensitive lipase. They have examined the phosphorylation state of cellular proteins under conditions of varying A-kinase activities in the presence and absence of insulin. Protein phosphorylation was determined by SDS-PAGE electrophoresis of extracts from /sup 32/P-loaded cells; glycerol and A-kinase activity ratios were measured in the cytosolic extracts from control, non-radioactive cells. Increased protein phosphorylation in general occurred over the same range of A-kinase activity ratios, 0.1-0.3, associated with increased glycerol release. The insulin-induced decrease in lipolysis was associated with a decrease in the /sup 32/P ...

1987-05-01

208

Inferring Stabilizing Mutations from Protein Phylogenies: Application to Influenza Hemagglutinin

The lysosomal catabolism of sulfatide requires arylsulfatase A and a specific sphingolipid activator protein, SAP-1. While most patients with metachromatic leukodystrophy have mutations in the gene...Full Text Available

1990-02-01

209

Individual Mycobacterium tuberculosis universal stress protein homologues are dispensable in vitro

One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions. We present...Full Text Available

2009-04-01

210

In vitro protein binding of cefonicid and cefuroxime in adult and neonatal sera.

SummaryMycobacterium tuberculosis has 10 universal stress proteins, whose function is unknown. However, proteomic and transcriptomic analyses have shown that a number...Full Text Available

2010-07-01

211

Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations?

The levels of in vitro protein binding of cefonicid and cefuroxime in human adult and neonatal sera were compared. Binding parameters for each drug were determined within the concentration range of...Full Text Available

1993-06-01

212

How Protein Stability and New Functions Trade Off

The Moloney murine leukemia virus (MMLV) belongs to the Retroviridae family of enveloped viruses, which is known to acquire minute amounts of host cellular proteins both on the surface...Full Text Available

2008-02-01

213

Functional properties of membrane cofactor protein of complement.

Numerous studies have noted that the evolution of new enzymatic specificities is accompanied by loss of the protein's thermodynamic stability (ΔΔG), thus suggesting...Full Text Available

2008-02-01

214

Fatty Acid- and Retinoid-binding Proteins Have Distinct Binding Pockets for the Two Types of Cargo*

Membrane cofactor protein (MCP or gp45-70) of the complement system is a cofactor for factor I-mediated cleavage of fluid-phase C3b and C3b-like C3, which opens the thioester bond. In the present study...Full Text Available

1989-12-01

215

Evidence for nonrandom hydrophobicity structures in protein chains.

Parasitic nematodes cause serious diseases in humans, animals, and plants. They have limited lipid metabolism and are reliant on lipid-binding proteins to acquire these metabolites from their hosts....Full Text Available

2009-12-18

216

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay

The question of whether proteins originate from random sequences of amino acids is addressed. A statistical analysis is performed in terms of blocked and random walk values formed by binary hydrophobic...Full Text Available

1996-09-03

217

Evaluation of Two Homologous Proline-Rich Proteins of Coccidioides posadasii as Candidate Vaccines against Coccidioidomycosis?

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis ...

1994-08-01

218

EhMAPK, the Mitogen-Activated Protein Kinase from Entamoeba histolytica Is Associated with Cell Survival

Evaluation of the protective efficacy of recombinant T-cell-reactive proteins of Coccidioides posadasii in a murine model of coccidioidomycosis has led to the discovery of potential...Full Text Available

2007-12-01

219

Effects of PKA phosphorylation on the conformation of the Na,K-ATPase regulatory protein FXYD1

Mitogen Activated Protein Kinases (MAPKs) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and...Full Text Available

220

Effects of Essential Oils on Ruminal Microorganisms and Their Protein Metabolism

FXYD1 (phospholemman) is a member of an evolutionarily conserved family of membrane proteins that regulate the function of the Na,K-ATPase enzyme complex in specific tissues and specific physiological...Full Text Available

2009-11-01

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221

E2f binding-deficient Rb1 protein suppresses prostate tumor progression in vivo

A commercial blend of essential oil (EO) compounds was added to a grass, maize silage, and concentrate diet fed to dairy cattle in order to determine their influence on protein metabolism by ruminal...Full Text Available

2003-08-01

222

Display of Recombinant Proteins on Bacillus subtilis Spores, Using a Coat-Associated Enzyme as the Carrier?

Mutational inactivation of the RB1 tumor suppressor gene initiates retinoblastoma and other human cancers. RB1 protein (pRb) restrains cell proliferation by binding...Full Text Available

2011-01-11

223

Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice

The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater...Full Text Available

2010-09-01

224

Determinants of the plasma protein binding of theophylline in health.

Uncoupling protein 2 (UCP2) maps to a region on distal mouse chromosome 7 that has been linked to the phenotypes of obesity and type II diabetes. We recently reported that UCP2 expression is increased...Full Text Available

1998-03-31

225

Dengue virus protein recognition by virus-specific murine CD8+ cytotoxic T lymphocytes.

1 The plasma protein binding of theophylline was determined after addition of [14C]-theophylline (15 micrograms/ml) to plasma from 24 healthy drug-free volunteers and equilibrium dialysis for 2 h at...Full Text Available

1983-04-01

226

Deletion of the Plasmodium falciparum Merozoite Surface Protein 7 Gene Impairs Parasite Invasion of Erythrocytes?

The identification of the protein targets for dengue virus-specific T lymphocytes may be useful for planning the development of subunit vaccines against dengue. We studied the recognition by murine...Full Text Available

1993-02-01

227

Degradation of Proteins Artificially Introduced into Vacuoles of Chara australis¹

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...Full Text Available

2008-12-01

228

Deglutathionylation of 2-Cys Peroxiredoxin Is Specifically Catalyzed by Sulfiredoxin*

When an exogenous protein, bovine serum albumin, was introduced into the vacuole of a Chara australis internodal cell, it was degraded with time. This degradation proceeded only in...Full Text Available

1988-12-01

229

Deficiency of circadian protein CLOCK reduces lifespan and increases age-related cataract development in mice

Reversible protein glutathionylation plays a key role in cellular regulation and cell signaling and protects protein thiols from hyperoxidation. Sulfiredoxin (Srx), an enzyme that catalyzes the reduction...Full Text Available

2009-08-28

230

DNA replication in Escherichia coli mutants that lack protein HU.

Circadian clock is implicated in the regulation of aging. The transcription factor CLOCK, a core component of the circadian system, operates in complex with another circadian clock protein BMAL1. Recently...Full Text Available

231

Cyclic Diguanylate Signaling Proteins Control Intracellular Growth of Legionella pneumophila

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

1989-10-01

232

Coupling of Pressure-Induced Structural Shifts to Spectral Changes in a Yellow Fluorescent Protein

Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell...Full Text Available

233

Controlling Gas-Phase Reactions for Efficient Charge Reduction Electrospray Mass Spectrometry of Intact Proteins

AbstractX-ray diffraction analysis of pressure-induced structural changes in the Aequorea yellow fluorescent protein Citrine reveals the structural basis for the continuous...Full Text Available

2009-09-16

234

Computer-based analysis of Haemophilus parasuis protein fingerprints

Charge reduction electrospray mass spectrometry (CREMS) reduces the charge states of electrospray-generated ions, which concentrates the ions from a protein into fewer peaks spread over a larger...Full Text Available

2005-11-01

235

Computer simulation of protein self-association during small-zone gel filtration. Estimation of equilibrium constants.

AbstractThe present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the...Full Text Available

2004-01-01

236

Complement Factor H-Related Proteins CFHR2 and CFHR5 Represent Novel Ligands for the Infection-Associated CRASP Proteins of Borrelia burgdorferi

A simulation is developed that qualitatively describes the small-zone-gel-filtration behaviour of a reversibly associating protein. The results reflect the dependence of the apparent molecular weight...Full Text Available

1981-04-01

237

Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues

BackgroundOne virulence property of Borrelia burgdorferi is its resistance to innate immunity, in particular to complement-mediated killing. Serum-resistant B....Full Text Available

238

Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis.

AIM: To investigate the relation of Fas and Fas ligand (FasL) protein expression with carcinogenesis and metastasis of gastric carcinoma.METHODS: Immunohistochemistry was used to detect Fas and...Full Text Available

2010-03-14

239

Circulating antibody to myelin basic protein in relapsing-remitting multiple sclerosis? A comparative group and sequential study by radioimmunoassay.

Cellular cholesterol homeostasis is controlled by sterol-regulated proteolysis of membrane-bound transcription factors called sterol-regulatory element binding proteins (SREBPs). CPP32, a cysteine protease,...Full Text Available

1996-03-01

240

Characterization of group A streptococcal T-12 protein purified by ion-exchange column chromatography.

Sera from multiple sclerosis patients with relapsing-remitting disease and normal subjects were tested for antibody to myelin basic protein by a sensitive radioimmunoassay. The results showed a marginally...Full Text Available

1978-12-01

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241

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

The aim of the present study was to describe the physicochemical characteristics of streptococcal T antigen. T protein isolated from Streptococcus pyogenes type 12 (R53/1077, Colindale) and purified...Full Text Available

1978-09-01

242

CID755673 enhances mitogenic signaling by phorbol esters, bombesin and EGF through a protein kinase D-independent pathway

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

243

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

Recently, CID755673 was reported to act as a highly selective inhibitor of protein kinase D (PKD). In the course of experiments using CID755673, we noticed that it exerted unexpected stimulatory...Full Text Available

2010-01-01

244

Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

245

Association of Protein Phosphatase 1?₁ with Spinophilin Suppresses Phosphatase Activity in a Parkinson Disease Model*

Certain pathogenic species of Bacillus and Clostridium have developed unique methods for intoxicating cells that employ the classic enzymatic “A-B” paradigm for protein toxins. The binary...Full Text Available

2004-09-01

246

Application of the PM6 semi-empirical method to modeling proteins enhances docking accuracy of AutoDock

Sustained nigrostriatal dopamine depletion increases the serine/threonine phosphorylation of multiple striatal proteins that play a role in corticostriatal synaptic plasticity, including Thr²⁸⁶...Full Text Available

2008-05-23

247

Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models

BackgroundMolecular docking methods are commonly used for predicting binding modes and energies of ligands to proteins. For accurate complex geometry and binding energy estimation,...Full Text Available

248

An automated procedure for detecting protein folds from sub-nanometer resolution electron density

PurposeCataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and...Full Text Available

249

Amyloid Oligomer Neurotoxicity, Calcium Dysregulation, and Lipid Rafts

The use of sub-nanometer resolution electron density as spatial constraints for denovo and ab-initio structure prediction requires knowledge of protein boundaries...Full Text Available

2010-06-01

250

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Amyloid proteins constitute a chemically heterogeneous group of proteins, which share some biophysical and biological characteristics, the principal of which are the high propensity to acquire an incorrect...Full Text Available

251

Albumin binding of insulins acylated with fatty acids: characterization of the ligand-protein interaction and correlation between binding affinity and timing of the insulin effect in vivo.

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

252

Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein.

Albumin is a multifunctional transport protein that binds a wide variety of endogenous substances and drugs. Insulins with affinity for albumin were engineered by acylation of the epsilon-amino group...Full Text Available

1995-12-15

253

Adhesion of DOPA-Functionalized Model Membranes to Hard and Soft Surfaces

To study the effect of agonist on the TRH (thyrotrophin-releasing hormone) receptor protein, an epitope-tagged receptor was stably expressed in HEK-293 cells (human embryonic kidney 293 cells) and receptor...Full Text Available

2004-06-15

254

Absence of Ataxin-3 Leads to Enhanced Stress Response in C. elegans

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to form strong and durable bonds in aqueous environments. These mussel adhesive proteins contain an unusual...Full Text Available

2009-01-01

255

AMP-activated protein kinase (AMPK) cross-talks with canonical Wnt signaling via phosphorylation of ?-catenin at Ser 552

Ataxin-3, the protein involved in Machado-Joseph disease, is able to bind ubiquitylated substrates and act as a deubiquitylating enzyme in vitro, and it has been involved in the modulation of protein...Full Text Available

256

AMP-Activated Protein Kinase: A Stress-Responsive Kinase with Implications for Cardiovascular Disease

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism; its activity is regulated by a plethora of physiological conditions, exercises and many anti-diabetic drugs. Recent...Full Text Available

2010-04-23

257

AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5

AMP-activated protein kinase (AMPK) was initially viewed as energy sensor and activated by increased intracellular concentrations of AMP following nutrient deprivation. Physiological or pathological...Full Text Available

2010-04-01

258

A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase

Amyloid precursor protein (APP), implicated in Alzheimer's disease, is a transmembrane protein of undetermined function. APP is cleaved by gamma-secretase that releases the APP intracellular domain...Full Text Available

259

A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

SummaryThe prevalence of paralogous enzymes implies that novel catalytic functions can evolve on preexisting protein scaffolds. The weak secondary activities of proteins, which...Full Text Available

2008-03-21

260

A microscale protein NMR sample screening pipeline

The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies...Full Text Available

2009-01-01

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261

A global optimization algorithm for protein surface alignment

As part of efforts to develop improved methods for NMR protein sample preparation and structure determination, the Northeast Structural Genomics Consortium (NESG) has implemented an NMR screening pipeline for protein target selection, construct optimization, and buffer optimization, incorporating efficient microscale NMR screening of proteins using a micro-cryoprobe. The process is feasible because the newest generation probe requires only small amounts of protein, typically 30-200 ?g in 8-35 ?l volume. Extensive automation has been made possible by the combination of database tools, mechanization of key process steps, and the use of a micro-cryoprobe that gives excellent data while requiring little optimization and manual setup. In this perspective, we describe the overall process used by the NESG for screening NMR samples as part of a sample optimization process, assessing optimal construct design and ...

2010-01-01

262

A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding

BackgroundA relevant problem in drug design is the comparison and recognition of protein binding sites. Binding sites recognition is generally based on geometry often combined with...Full Text Available

263

A gene encoding a protein modified by the phytohormone indoleacetic acid

BackgroundProteins may evolve through the recruitment and modification of discrete domains, and in many cases, protein action can be dissected at the domain level. PDZ domains are...Full Text Available

264

?Activation of the mitogen-activated protein kinase pathways by heat shock

We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this...Full Text Available

2002-02-05

265

'Sheltered disruption' of Neurospora crassa MOM22, an essential component of the mitochondrial protein import complex.

In addition to inducing new transcriptional activities that lead within a few hours to the accumulation of heat shock proteins (Hsps), heat shock activates within minutes the major signaling transduction...Full Text Available

2002-04-01

266

[ ${\\alpha}$ ]-Amylase and Protein Tyrosine Phosphatase 1B Inhibitory of Some Vietnamese Medicinal Plants Used to Treat Diabetes

MOM22 is a component of the protein import complex of the mitochondrial outer membrane of Neurospora crassa. Using the newly developed procedure of 'sheltered disruption', we created a heterokaryotic...Full Text Available

1995-03-15

267

Uptake of Heavy Metal Ions by Water Dropwort (Oenanthe stolonifera DC.) and Identification of Its Heavy Metal-Binding Protein

Full Text Available

2007-12-01

268

The protein truncation caused by fusion of PEP-1 peptide and protective roles of transduced PEP-1-MsrA in skin cells

Full Text Available

1996-12-01

269

Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

Full Text Available

2011-04-01

270

Photolabelling reveals differences between cholera toxin and ricin toxin insertion mechanisms

Full Text Available

2009-10-01

271

Liquid nitrogen dewar for protein crystal growth

... carbon 14 cholera gels labelling membranes molecules proteins receptors

1982-07-28

272

Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the capsid protein from Rous sarcoma virus

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California,

2001-01-01

273

Interaction Of Human La Protein With The Internal Ribosome Entry Site Of Hepatitis C Virus : Functional Role In Mediating Internal Initiation Of Translation

No abstract prepared.

1999-11-15

274

Indian Institute of Science Theses & Dissertations (India)

Full Text Available

2004-07-01

275

Identification of Protective Brucella Antigens and their ...

Heavy Metals Effect on Animal Cells

... Considering these observations, it is most likely that Brucela proteins involved in protective immunity will preferentially stimulate INF-g producing T ...

1996-05-01

276

International Science & Technology Center (ISTC)

Studies of the Superheavy-metal Effect as a Stress-factor on Protein Biosynthesis and Cytoskeleton Integrity in Animal Cells

277

Functional characterization of a CCCH type zinc-finger protein gene OsZF2 by ectopic overexpression of the gene in rice

Free radicals and their transformations in irradiated proteins

Full Text Available

2009-03-01

278

Effect of protein malnutrition on the metabolism of bone collagen in albino rats

Experimental investigation data are systematized of free radical states and processes in irradiated proteins. The investigation is performed by the radiospectral methods. Results are discussed in detail of the study of free radicals electronic structure of amino acids, peptides and proteins formed by the action of ionizing radiation. The specificity is stressed of the study of monocrystalls of these compounds by the method of electronic paramagnetic resonance. The nature is also studied of primary centres formed under the effect of radiation on biologically important compounds and their subsequent reactions in solid and liquid solutions. Ion-radical states of different functional groups of the protein molecule are studied. Prospects of the study and the role of anion-radicals in biological processes are discusses.

279

Effect of Carbon Tetrachloride on the Changes of Xanthine Oxidase Activity in Rate Previously Fed Low or High Protein Diet

The effect of protein malnutrition on the metabolism of collagen in bone was studied in young female albino rats after a single injection of "3H-proline. Both specific and total radioactivities of hydroxyproline in the total collagen of the bone were found to decrease in the protein-deficient animals, indicating decreased rate of collagen synthesis. In the urine the amount of hydroxyproline excreted and total radioactivity of "3H-hydroxyproline were greatly decreased. The results of the present investigation therefore clearly indicate decreased synthesis and catabolism of collagen in bones of protein deficient animals compared to controls. (auth.).

1981-01-01

280

Turnover of whole body proteins and myofibrillar proteins in middle-aged active men

Full Text Available

1991-12-01

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281

Tomato 14-3-3 Protein 7 Positively Regulates Immunity-Associated Programmed Cell Death by Enhancing Protein Abundance and Signaling Ability of MAPKKK ?^[C]^[W]

Endurance-trained older men have a higher proportion of lean tissue and greater muscle cell oxidative capacity, reversing age-related trends and suggesting major changes in protein metabolism. In this study, protein turnover was determined in 6 middle-aged (52+/-1 yr) men who were well trained (VO_2 max 55.2+/-5.0 ml O_2/kg.min) and lean (body fat 18.9+/-2.8%, muscle mass 36.6+/-0.6%). The maintained habitual exercise while consuming 0.6, 0.9 or 1.2 g protein/kg.day for 10-day periods. N flux was measured from "1"5N in urea after oral "1"5N-glycine administration. Myofibrillar protein breakdown was estimated from urinary 3-methyl-histidine. Dietary protein had no effect on turnover rates, even when N balance was negative. Whole body protein synthesis was 3.60+/-0.12 g/kg.day and breakdown was 3.40+/-0.14 g/kg.day for all N intakes. Whole body ...

1986-04-13

282

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis^[W]^[OA]

Programmed cell death (PCD) is triggered when Pto, a Ser-Thr protein kinase, recognizes either the AvrPto or AvrPtoB effector from Pseudomonas syringae pv tomato. This...Full Text Available

2010-01-01

283

Scyl1 Facilitates Nuclear tRNA Export in Mammalian Cells by Acting at the Nuclear Pore Complex

In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal...Full Text Available

2010-05-01

284

Relationships of Quality Characteristics with Size Exclusion HPLC Chromatogram of Protein Extract in Soft-White Winter Wheats.

Scyl1 is an evolutionarily conserved N-terminal protein kinase-like domain protein that plays a role in COP1-mediated retrograde protein trafficking in mammalian cells. Furthermore, loss of Scyl1 function...Full Text Available

2010-07-15

285

Protective Effects of a Human 18-Kilodalton Cationic Antimicrobial Protein (CAP18)-Derived Peptide against Murine Endotoxemia

This study investigated relationships between molecular weight distributions of unreduced grain proteins and grain, flour, and end-use quality characteristics of soft white winter wheats grown in Oregon. Absorbance area and area % values of protein fractions separated by size exclusion HPLC (SE-HPL...

286

Influence of the high-affinity growth hormone (GH)-binding protein on plasma profiles of free and bound GH and on the apparent half-life of GH. Modeling analysis and clinical applications.

CAP18 (an 18-kDa cationic antimicrobial protein) is a granulocyte-derived protein that can bind lipopolysaccharide (LPS) and inhibit various activities of LPS in vitro. The present study examined the...Full Text Available

1998-05-01

287

Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

The discovery of a specific high-affinity growth hormone (GH) binding protein (GH-BP) in plasma adds complexity to the dynamics of GH secretion and clearance. Intuitive predictions are that such a protein...Full Text Available

1993-02-01

288

Gamma interferon induces rapid and coordinate activation of mitogen-activated protein kinase (extracellular signal-regulated kinase) and calcium-independent protein kinase C in human monocytes.

We have identified another Drosophila GTP-binding protein (G protein) alpha subunit, dGq alpha-3. Transcripts encoding dGq alpha-3 are derived from alternative splicing of the dGq alpha locus previously...Full Text Available

1995-12-05

289

Dual Functions of ASCIZ in the DNA Base Damage Response and Pulmonary Organogenesis

Gamma interferon plays an important role in regulating the functional properties of mononuclear phagocytes. In the present study, the role of activated protein kinases in the mechanism of action of...Full Text Available

1994-07-01

290

Chemical and Immunological characterization of the major structural protein (p28) of MMC-1, a rhesus monkey endogenous type C virus: homology with the major structural protein of avian reticuloendotheliosis virus.

Zn²⁺-finger proteins comprise one of the largest protein superfamilies with diverse biological functions. The ATM substrate Chk2-interacting Zn²⁺-finger protein...Full Text Available

2010-10-01

291

Wind or water driven wheel and bird scarer

The major core protein (p28) of MMC-1, an endogenous type C virus of the rhesus monkey (Macaca mulatta), was purified and subjected to structural and immunological analyses. The NH2-terminal amino acid...Full Text Available

1981-09-01

292

Transition rates of electrons in superheavy elements

The wind unit working wheel has 4 radial rectangular frames arranged in two vertical planes which are perpendicular to each other. In the planes limited by the frames, on loop hinges with limiting supports, there are flat blades. When the working wheel rotates under the influence of wind pressure, the blades automatically occupy a vertical or horizontal (wind vane) position depending on the direction of blade motion and the direction of wind velocity.

1984-01-04

293

The influence of the nonhomogeneous temperature field on the optimal winding prestress of the compound pressure vessel

Transition rates for electrons in the superheavy elements Z = 114, 126, 134, 145, 164 and 173 are calculated. K, L and M-shells are considerd as final states. The 2s - 1s stransition of multipolarity M1 is dominant for Z = 173 with a transition time of 10"-"1"8s. The radial expectation values and #sq root# are given. (orig.).

294

The general relativistic Poynting-Robertson effect

The completed ribbon-winding theory, including temperature loading of the high-pressure compound vessel, was proposed. The influence of the bending stress in the wound ribbon is taken into account. Internal core of the compound vessel consists of matrix and ring, made of different materials. The relations were derived in analytical form, describing the radial distribution of necessary tangential prestressing.

1996-11-01

295

The (2+1) Dirac Equations with $\\delta$ Potential

The general relativistic version is developed for Robertson's discussion of the Poynting-Robertson effect that he based on special relativity and Newtonian gravity for point radiation sources like stars. The general relativistic model uses a test radiation field of photons in outward radial motion with zero angular momentum in the equatorial plane of the exterior Schwarzschild or Kerr spacetime.

2009-03-07

296

Test particle moving in an interior static spherically symmetric geometry

In this Letter the bound states of (2+1) Dirac equation with the cylindrically symmetric $\\delta (r-r_{0})$-potential are discussed. It is surprisingly found that the relation between the radial functions at two sides of $r_{0}$ can be established by an SO(2) transformation. We obtain a transcendental equation for calculating the energy of the bound state from the matching condition in the configuration space. The condition for existence of bound states is determined by the Sturm-Liouville theorem.

2001-01-01

297

Scattering of scalar tardyons and tachyons from a Schwarzschild black hole

The motion of a test particle moving in an interior static spherically symmetric geometry is investigated. The author concludes that an oscillatory motion with the test particle changing its motion from a free fall towards the center to an outward radial motion can not exist for any static interior spherically symmetric model.

1984-06-01

298

Scattering of scalar tardyons and tachyons from a Schwarzschild black hole

The Klein-Gordan equation in the background of the Schwarzschild curved space-time is considered and the scattering of radial tardyons and tachyons from a black hole is studied. It is shown that black holes of mass below 7x10/sup 14/g may contain bound states of tardyons of pion mass which will be unstable on account of the presence of an attractive r/sup -4/ term.

1982-10-01

299

Role of core toroidal rotation on the H-mode radial electric field shear, turbulence, and confinement as studied by magnetic braking in the DIII-D tokamak

The Klein-Gordan equation in the background of the Schwarzschild curved space-time is considered and the scattering of radial tardyons and tachyons from a black hole is studied. It is shown that black holes of mass below 7x10"1"4g may contain bound states of tardyons of pion mass which will be unstable on account of the presence of an attractive r"-"4 term. (author).

300

Proton beam therapy for cancer in deep-seated organs

''Magnetic braking'' of the plasma toroidal rotation in the high confinement H mode by applied resonant, low m,n=1 static error fields is used in DIII-D [Nucl. Fusion 31, 875 (1991)] as an independent control to evaluate the E_rxB stabilization of microturbulence in the plasma core. In the core (#rho# approx-lt 0.9) of a tokamak, the radial electric field and its shear are dominated by toroidal rotation. The fundamental quantity for shear stabilization of microturbulence is shear in the velocity of the fluctuations v_p_e_r_p_e_n_d_i_c_u_l_a_r#approx#E_rxB/B#centre dot#B which in the core is v_p_e_r_p_e_n_d_i_c_u_l_a_r#approx#v_#phi#B_#theta#/ B_#phi#. With magnetic braking greatly decreasing the toroidal rotation and thus reducing the core radial electric field and shear, far infrared (FIR) measurements of density microturbulence show downshifting in frequency near #rho##approx#0.8 as a result of the reduced Doppler shift ...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

On the GBDT version of the B\\"acklund-Darboux transformation and its applications to the linear and nonlinear equations and Weyl theory

Radiation therapy has shown definite technical improvement due to application of proton beam irradiation. Clinical results of proton beam therapy also suggested enlargement of indication ranges in radial treatment and decrease of post radiation damages of the treatment of cancers in deep-seated organs. Inprovement of result in cancer therapy could be expected by this treatment technique in the future.

1988-10-01

302

Lorentz noninvariance without tachyons in the Schwarzschild field

A general theorem on the GBDT version of the B\\"acklund-Darboux transformation for systems rationally depending on the spectral parameter is treated and its applications to nonlinear equations are given. Explicit solutions of direct and inverse problems for Dirac-type systems, including systems with singularities, and for the system auxiliary to the $N$-wave equation are reviewed. New results on explicit construction of the wave functions for radial Dirac equation are obtained.

2009-01-01

303

Estimation of errors provided with dead times of roentgen digital tomography

Considering the radial motion of a test particle in the Schwarzschild field in the hypothesis of local rotational invariance, the authors discuss the conditions to be satisfied by a physically acceptable model of broken SO(3,1) symmetry, and shown in particular that to preserve causality a breaking, even infinitesimal, makes necessarily impenetrable the interior of the Schwarzschild sphere.

1987-06-01

304

Effect of rapid thermal annealing on radiation hardening of MOS devices

Impact of apparatus dead time on the accuracy of measurement of radial distribution of the radiation attenuation factor in the roentgen computer tomography for rotation bodies is studied. Analytical expressions are obtained. Calculations of the threshold loads for radiometric detectors, providing for the accuracy of measurement at the stage of designing the tomographic complex detecting unit, are obtained

305

Compton scatter tomography and its inversion using a few projections

The influence of RTA (Rapid Thermal Anneal) treatment on MOS radiation hardness is demonstrated and compared with classical furnace treatment. In the case of the RTA, the oxide trapped charge is found to depend on: (i) the anneal temperature as expected, data are in good agreement with a recently developed model of oxygen out-diffusion; (ii) the location across the wafer with a radial dependence, results could be related to stress induced by thermal gradient.

1995-07-17

306

A microscopic description of neutron-rich lithium isotopes

Compton scatter tomography utilizes the electronic collimation characteristics available through the Compton scattering angle-energy correlation to obtain tomographic images. In this work we present particular aspects of the technique, which are relevant to the inverse radiation transport problem of reducing marginal projection data to radial two-phase flow regime maps. The results indicate a viable technique for the tomographic imaging of tow-phase flow using practical source strengths and reasonably few detectors.

1988-01-01

307

Study of protein-protein interactions in under saturated and supersaturated lysozyme solutions in heavy water as a function of temperature; Etude des interactions proteine-proteine en solutions sous-saturees et sursaturees de lysozyme dans l`eau lourde en fonction de la temperature

A unified calculation of neutron-rich isotopes in lithium is performed using the hyperspherical basis in which the underlying symmetry of each isotope exhibits a simple structure. The variation of the binding energy as a function of mass number is qualitatively reproduced, and the asymptotic of radial distribution of each isotope decreases exponentially. The form factors of the lithium isotopes are calculated and display diffraction minima. 27 refs., 3 figs., 3 tabs.

308

Observation of the study of protein metabolism in relation to nutrition

We have studied freshly prepared lysozyme solutions in heavy water for two NaCl concentrations as a function of temperature. Lysozyme solubilities in this solvent are determined by static light scattering. By small angle neutron scattering, we evidence that interactions between lysozyme molecules are characterized by a second virial coefficient A{sub 2} whether the solution is under-saturated or supersaturated. From the variation of A{sub 2} as a function of temperature we have evaluated the enthalpy corresponding to the interaction between lysozyme molecules. We show that the interactions between protein molecules are higher in heavy water than in light water. (authors). 13 refs., 3 figs.

1996-04-01

309

Identification of a functional nuclear export signal in the green fluorescent protein asFP499

It is a useful hypothesis to thing of the reaction to protein deprivation as made up of two parts. First there is an initial adaptation involving rapid losses of protein. This is followed by slow continuing depletion. When considering two organ groups, such as the liver and muscle, it appears that the curve of urinary nitrogen excretion reflects the different parts they play in these two phases. The changes in the relative amounts of protein in different organs results in an alteration in the pattern of body protein at the level of the organ, the tissue and the cell. These changes are themselves the result of altered rates of synthesis and breakdown. The questions that must be asked and hopefully will be answered with the help of isotopes, are how these changes are brought about, how they are inter-related and controlled, and what is their significance? This paper contains a discussion of the few ...

1965-06-01

310

Artificial neural network modeling of physicochemical changes of shrimp during boiling

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

311

British Library Electronic Table of Contents (United Kingdom)

Frozen boiled shrimp and dried shrimp are among the high-value fishery products of Thailand. During the production of these products boiling is one of the most important steps that affects significantly the product physicochemical properties, especially the quantity and quality of proteins, which in turn affect other apparent properties perceived by consumers. The protein changes are, however, difficult to evaluate comparing to other typical physical properties of shrimp. The objective of this study was therefore to develop an artificial neural network (ANN) model to predict the protein changes of shrimp in terms of protein loss and protein denaturation as a function of the boiling conditions, namely, concentration of salt solution and boiling time, as well as a rather easily determined ch...

2012-01-01

312

Amyloid oligomers: spectroscopic characterization of amyloidogenic protein states

British Library Electronic Table of Contents (United Kingdom)

It is assumed that protein fibrils manifested in amyloidosis result from an aggregation reaction involving small misfolded protein sequences being in an `oligomeric' or `prefibrillar' state. This review covers recent optical spectroscopic studies of amyloid protein misfolding, oligomerization and amyloid fibril growth. Although amyloid fibrils have been studied using established protein-characterization techniques throughout the years, their oligomeric precursor states require sensitive detection in real-time. Here, fluorescent staining is commonly performed using thioflavin T and other small fluorescent molecules such as 4-(dicyanovinyl)- julolidine and 1-amino-8-naphtalene sulphonate that have high affinity to hydrophobic patches. Thus, populated oligomeric intermediates and related `pre...

2010-01-01

313

Altered protein phosphorylation in sciatic nerve from rats with streptozocin-induced diabetes

Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells

The effect of experimental diabetes on the phosphorylation of proteins in the rat sciatic nerve was studied. Nerves from animals made diabetic with streptozocin were incubated in vitro with (/sup 32/P)orthophosphate and divided into segments from the proximal to the distal end, and proteins from each segment were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The principal labeled species were the major myelin proteins, P0, and the basic proteins. After 6 wk of diabetes, the incorporation of isotope into these proteins rose as a function of distance along the nerve in a proximal to distal direction and was significantly higher at the distal end compared with incorporation into nerves from age-matched controls. The overall level of isotope uptake was similar in nerves from diabetic animals and weight-matched controls. The distribution of /sup 32/P among ...

1987-11-01

314

Euglena light-harvesting chlorophyll A/B binding protein (LHCP) synthesized as an unusually large precursor

The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10 nM of Cd for 24 h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10 nM. By two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The ...

2008-11-20

315

The Effects of Surface Chemistry on the Properties of Proteins Confined in Nano-porous Materials

Light increased the rate of LHCP synthesis as measured by pulse-labeling with /sup 35/SO/sub 4/ and immunoprecipitation with antibody specific for Euglena LHCP. In addition to the mature LHCP, 26,000 daltons, the LHCP specific antibody immunoprecipitated large amounts of several proteins having molecular weights of approximately 100,000. On immunoblots of immunoprecipitated unlabeled protein, the antibody only detected the mature LHCP suggesting that the high molecular weight proteins are not LHCP aggregates produced during immunoprecipitation. After a 10 min pulse with /sup 35/SO/sub 4/, the 100,000 dalton proteins constituted over 80% of the immunoprecipitated material. In a subsequent chase, the radioactivity in the 100,000 dalton proteins decreased and the radioactivity in the mature LHCP increased suggesting a precursor-product relationship. After a 35 minute chase, the mature ...

1987-04-01

316

Single protein omission reconstitution studies of tetracycline binding to the 30S subunit of Escherichia coli ribosomes

The entrapment of proteins using the sol-gel route provides a means to retain its native properties and artificially reproduce the molecular crowding and confinement experienced by proteins in the cell allowing investigation of the physico-chemical and structural properties of biomolecules at the biotic/abiotic interface. The biomolecules are spatially separated and 'caged' in the gel structure but solutes can freely permeate the matrix. Thus, properties such as the folding of ensembles of individual molecules can be examined in the absence of aggregation effects that can occur in solution studies. Green fluorescent protein from Aequorea coerulescens was used as a model protein to examine the unfolding/re-folding properties of protein in silica gels. The recombinant protein was isolated and purified from Escherichia coli extracts by cell lysis, ...

2007-09-01

317

p53 protein in aggressive and non-aggressive basal cell carcinoma.

In previous work the authors showed that on photolysis of Escherichia coli ribosomes in the presence of ({sup 3}H)tetracycline (TC) the major protein labeled is S7, and they presented strong evidence that such labeling takes place from a high-affinity site related to the inhibitory action of TC. In this work they use single protein omission reconstitution (SPORE) experiments to identify those proteins that are important for high-affinity TC binding to the 30S subunit, as measured by both cosedimentation and filter binding assays. With respect to both sedimentation coefficients and relative Phe-tRNA{sup Phe} binding, the properties of the SPORE particles they obtain parallel very closely those measured earlier, with the exception of the SPORE particle lacking S13. A total of five proteins, S3, S7, S8, S14, and S19, are shown to be important for TC binding, with the largest effects seen on omission of ...

1990-06-05

318

Proteomic Analysis of Proteins Secreted by HepG2 Cells Treated with Butyl Benzyl Phthalate

Basal cell carcinoma (BCC) is the most frequent cutaneous neoplasm, with a generally favorable clinical behavior. Sometimes, indeed, it recurs after therapy and/or metastasizes. As point mutations in the coding sequence of the p53 tumor suppressor gene have been implicated in the progression of many human tumors, we studied the expression of p53 protein on this neoplasia. We tested immunohistochemically the positivity for p53 protein (NCL-p53-CM1, YLEM) on 19 cases of morphologically "non aggressive" BCC (BCC1) and on 19 "aggressive" BCC (BCC2), all with one or more relapses and 3 with distant metastases also. Results were related to clinico-pathological and follow-up data. All but one BCC2 were found positive for p53 protein. Conversely, only 2 cases of BCC1 exhibited low immunoreactivity for p53 protein, with high statistical differences between the two groups. No correlation was found between the ...

1993-10-01

319

British Library Electronic Table of Contents (United Kingdom)

Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 ?M) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate ...

2010-01-01

320

Macronutrient self-selection in Solea senegalensis fed macronutrient diets and challenged with dietary protein dilutions

British Library Electronic Table of Contents (United Kingdom)

Macronutrient self-selection and responses to dietary protein dilution were investigated in Senegalese sole, a flatfish species of interest for Mediterranean aquaculture. Eight groups of six sole were distributed among 75-l tanks and allowed to feed on three experimental diets composed of pure macronutrients: PC (75% crude protein and 25% carbohydrate), PF (75% crude protein and 25% crude fat) and PFC (10% crude protein, 45% crude fat and 45% carbohydrate). Choosing from the above diets, the sole selected a diet containing 68.0% crude protein, 15.7% crude fat and 16.3% carbohydrate. When the PF diet was diluted 50% with cellulose, the fish increased their feed intake to sustain the energy intake (5.0?kJ/kg BW/day). Their selection of fat remained unchanged, while carbohydrate selection sig...

2009-01-01

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321

Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori

British Library Electronic Table of Contents (United Kingdom)

Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gus...

2011-01-01

322

Human complement protein C99 is a calcium binding protein

Human complement protein C9 is shown to be a metalloprotein that binds 1 mol of Ca"2"+/mol of C9 with a dissociation constant of 3 #mu#m as measured by equilibrium dialysis. Incubation with EDTA removes the bound calcium, resulting in a apoprotein with decreased thermal stability. This loss in stability leads to aggregation and, therefore, to loss of hemolytic activity upon heating to a few degrees above the physiological temperature. Heat-induced aggregation of apoC9 can be prevented by salts that stabilize proteins according to the Hofmeister series of lyotropic ions, suggesting that the ion in native C9 may ligand with more than one structural element of domain of the protein. Ligand blotting indicates that the calcium binding site is located in the amino-terminal half of the protein. Removal of calcium by inclusion of EDTA in assay mixtures has no effect on the hemolytic activity of C9, and its ...

323

Amyloidosis

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding

The subjects covered in this Symposium range through almost every clinical medical specialty. From an average of one paper in each of the past three Symposiums, the explosive interest in cerebral amyloidosis has led to the presentation of 12 papers on this subject in the present volume. The genetically predisposed familial amyloidotic processes, such as the polyneuropathies and familial Mediterranean fever have also stimulated extensive and intriguing investigations which have revealed the striking effect of a single amino acid substitution in transforming a normal protein into a lethal ''amyloidogenic'' one. This Symposium clearly depicts the advances since the first amyloid fibril protein was definitively identified and defined 14 years ago. Since all amyloid fibril proteins so far described are variants of normal proteins, attention to gene abnormalities now becomes a significant focus as well as the ...

1984-11-09

324

Vibrational Circular Dichroism: A developing spectroscopic technique for peptide and protein conformational studies

The authors have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for ...

325

Fluoride induced changes in protein metabolism in the tissues of freshwater crab Barytelphusa guerini.

Electronic circular dichroism (ECD) of the n-#pi# and #pi# -#pi# transitions of the amide groups in the ultraviolet has become an indispensable tool for qualitative characterization of proteins in solution. Since the existence of such a spectrum (that is, of natural optical activity) comes from the three dimensional interaction of the chromophores in the molecule, CD is exquisitely sensitive to molecular conformation. However, in proteins, the amide transitions available in the near UV are limited in number and are broads and overlapping. Interactions among them yield information about the polymeric backbone, but since such amide electronic excitation are relatively delocalized, the resulting

1996-11-01

326

Cysteine-containing peptides having antioxidant properties

Exposure of the freshwater field crab Barytelphusa guerini to a sublethal concentration of NaF (30 mg litre(-1)) caused significant alterations in protein metabolism. After an exposure time of 15 days, the crab was found to have a marked depletion of total protein and free amino acid content. A significant elevation in transaminases, Aspartate aminotransferase (AAT) and Alanine aminotransferase (ALAT), and Glutamate dehydrogenase (GDH) activities was reflected in the free amino acid levels of the tissues. PMID:15092215

1990-01-01

327

Wnt/b-catenin signal pathway stabilizes APP intracellular domain (AICD) and promotes its transcriptional activity

The term "homology" or "homologous" means an amino acid similarity measured by the program, BLAST (Altschul et al (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:33 89 3402), and expressed as --(% identity n/n). In measuring homology between a peptide and a protein of greater size, homology is measured only in the corresponding region; that is, the protein is regarded as only having the same general length as the peptide, allowing for gaps and insertions.

2007-05-15

328

British Library Electronic Table of Contents (United Kingdom)

Amyloid precursor protein (APP), a key protein in pathogenesis of Alzheimer's disease (AD), is a type I transmembrane protein which can be cleaved by b- and g-secretase to release the amyloidogenic b-amyloid peptides (Ab) and the APP intracellular domain (AICD). While Ab has been widely believed to initiate pathogenic cascades culminating AD, the physiological functions and regulations of AICD remain elusive. In present study, endogenous AICD was demonstrated to be increased by canonical Wnt signal. Instead of due to g-secretase activity, enhanced AICD expression was found due to the increased protein stability by Wnt/b-catenin. b-Catenin was demonstrated to be an associating partner of AICD, capable of promoting AICD mediated transcriptional activity. Investigation by AICD mutants proved ...

2011-01-01

329

The actin content of fibroblasts.

Tau inhibits tubulin oligomerization induced by prion protein

Cultures of chick skin fibroblasts were dissolved in solutions of sodium dodecyl sulphate, and their entire protein content was examined by gel electrophoresis. The most abundant species migrated in...Full Text Available

1975-05-01

330

British Library Electronic Table of Contents (United Kingdom)

In previous studies we have demonstrated that prion protein (PrP) interacts with tubulin and disrupts microtubular cytoskeleton by inducing tubulin oligomerization. These observations may explain the molecular mechanism of toxicity of cytoplasmic PrP in transmissible spongiform encephalopathies (TSEs). Here, we check whether microtubule associated proteins (MAPs) that regulate microtubule stability, influence the PrP-induced oligomerization of tubulin. We show that tubulin preparations depleted of MAPs are more prone to oligomerization by PrP than those containing traces of MAPs. Tau protein, a major neuronal member of the MAPs family, reduces the effect of PrP. Importantly, phosphorylation of Tau abolishes its ability to affect the PrP-induced oligomerization of tubulin. We propose that t...

2011-01-01

331

Stochastic gene expression and its consequences

Site-Specific Methylation of the Promoter Alters Deoxyribonucleic Acid-Protein Interactions and Prevents ...

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

332

Refilling Intracellular Calcium Stores

... and R. L. Momparler. DNA methylation of retinoic acid receptor beta in breast cancer and possible therapeutic role of ... ...

333

Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

Within the cardiac cell, the movements of calcium ions are tightly regulated by a number of regulatory proteins including pumps, and channels. The sarcoplasmic reticulum (SR) is in large part...Full Text Available

2010-01-01

334

Protein expression profile from the Mesocestoides corti (Platyhelminthes: Cestoda) larval stage

Full Text Available

335

Pressure Stabilization of Proteins from Extreme Thermophiles

English 2007 [1 p.] Brazil Laschuk, A. Silveira, PA da Bizarro, CV Carvalho,

2007-05-21

336

Possible Implications of Placental Expressions for Heat Shock Protein 70 and Lectin-like Oxidized Low Density Lipoprotein Receptor-1 on Postnatal Blood Pressure in Their Newborns

We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published...Full Text Available

1994-03-01

337

Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin

Full Text Available

2007-09-01

338

Phylogenomic analyses of malaria parasites and evolution of their exported proteins

Eukaryotic genomes are packaged in two basic forms, euchromatin and heterochromatin. We have examined the composition and organization of Drosophila melanogaster heterochromatin in...Full Text Available

2011-02-01

339

Phosphorylation of Annexin A1 by TRPM7 Kinase: A Switch Regulating the Induction of an ?-Helix

BackgroundPlasmodium falciparum is the most malignant agent of human malaria. It belongs to the taxon Laverania, which includes other ape-infecting Plasmodium...Full Text Available

340

PUTATIVE CSF PROTEIN BIOMARKER CANDIDATES FOR AMNESTIC MILD COGNITIVE IMPAIRMENT

TRPM7 is an unusual bifunctional protein consisting of an α-kinase...Full Text Available

2011-03-29

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341

PHENOTYPIC MODIFICATION OF ROACH (RUTILUS RUTILUS L.) INFECTED WITH LIGULA INTESTINALIS L. (CESTODA: PSEUDOPHYLLIDEA)

The identification of individuals at risk for Alzheimer's disease (AD) is essential for the timely administration of treatment approaches aimed at slowing the onset or progression of the disease....Full Text Available

2010-03-01

342

Ovine reference materials and assays for prion genetic testing

... growth hormone-like factor from plerocercoids of the tapeworm Spirometra mansonoides is a multifunctional protein. In Parasites and pathogens: ... ...

343

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

344

Orphan GPCR research

Mutations in RNA Binding Protein Gene Cause Familial Dilated Cardiomyopathy

Orphan G protein-coupled receptors (GPCRs) are receptors lacking endogenous ligands. Found by molecular biological analyses, they became the roots of reverse pharmacology, in which receptors are attempted...Full Text Available

2008-03-01

345

Liver Carcinogenesis Unit

ObjectivesWe sought to identify a novel gene for dilated cardiomyopathy (DCM).BackgroundDCM is a heritable, genetically...Full Text Available

2009-09-01

346

Inhibition of heat shock protein 27 (HspB1) tumorigenic functions by peptide aptamers

The Liver Carcinogenesis Section uses the modern technologies of molecular biology, cell biology, protein chemistry and genetics to investigate the biochemical and genetic pathways involved in human liver cancer.

347

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

2011-01-01

348

Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin

Identification of Contractile Vacuole Proteins in Trypanosoma cruzi

The tetrapeptide KDEL is commonly found at the C terminus of soluble proteins of the endoplasmic reticulum (ER), and it contributes to their localization by interacting with a receptor that recycles...Full Text Available

2001-05-01

349

Hydrophobic labelling of membrane-embedded proteins with lipophilic reagents

Contractile vacuole complexes are critical components of cell volume regulation and have been shown to have other functional roles in several free-living protists....Full Text Available

350

High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead

Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymhocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of ["1"2"5I]iodonaphtylazide ["1"2"5I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymhpocytes with ["1"2"5I]INA and commercially available reagent 3-(trifluoromethyl)-3-(trifluoromethyl)-3-(3'-["1"2"5Iliodophenyl)diazirine (["1"2"5I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. (author). 28 refs.; 4 figs.

351

High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead

The PbII binding characteristics of the previously reported PbII binding proteins of rat kidney cytosol were investigated further. Saturation and Scatchard analysis of /sup 203/Pb binding in whole cytosol and in 40% saturated ammonium sulfate precipitated fractions disclosed a class of relatively high-affinity sites with an apparent Kd of approximately 50 nM and binding capacities of approximately 41 and 9 pmol/mg of protein, respectively. Two /sup 203/Pb binding proteins with approximate molecular masses of 63K and 11.5K daltons and a high molecular weight component (greater than 200K) were isolated by Sepharose-6B column chromatography. The time course of association of /sup 203/Pb with cytosol and the 63K protein showed maximum binding at 18 hr which was stable up to 25 hr at 4 degrees C. The approximate half-time dissociation rate (T 1/2) of specifically bound /sup 203/Pb to the 63K ...

1985-02-01

352

HDL in humans with cardiovascular disease exhibits a proteomic signature

The PbII binding characteristics of the previously reported PbII binding proteins of rat kidney cytosol were investigated further. Saturation and Scatchard analysis of "2"0"3Pb binding in whole cytosol and in 40% saturated ammonium sulfate precipitated fractions disclosed a class of relatively high-affinity sites with an apparent Kd of approximately 50 nM and binding capacities of approximately 41 and 9 pmol/mg of protein, respectively. Two "2"0"3Pb binding proteins with approximate molecular masses of 63K and 11.5K daltons and a high molecular weight component (greater than 200K) were isolated by Sepharose-6B column chromatography. The time course of association of "2"0"3Pb with cytosol and the 63K protein showed maximum binding at 18 hr which was stable up to 25 hr at 4 degrees C. The approximate half-time dissociation rate (T 1/2) of specifically bound "2"0"3Pb to the 63K protein ...

353

Favism, with special reference to Iran*

BackgroundAlterations in protein composition and oxidative damage of high-density lipoprotein (HDL) have been proposed to impair the cardioprotective properties of...Full Text Available

2010-07-04

354

Evaluation of the effect of Maytenus ilicifolia on the labelling of red blood cells and plasma proteins with Technetium-99m

Fava beans (Vicia fava) are cultivated rather widely in most countries of the Eastern Mediterranean area and provide a cheap but protein-rich food that can be eaten alone, in various...Full Text Available

1969-01-01

355

Evaluating concentration estimation errors in ELISA microarray experiments

English 1998 p. 196 Brazil Oliveira, JF Braga, ACS Santos, JS Bernardo Filho,

1998-05-23

356

Diagnostic Value of C-reactive Protein in Complicated Appendicitis

BackgroundEnzyme-linked immunosorbent assay (ELISA) is a standard immunoassay to estimate a protein's concentration in a sample. Deploying ELISA in a microarray format permits simultaneous...Full Text Available

357

Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance

PurposeEarly detection of appendicitis has increased due to development of computed tomography and ultrasonography, yet we are frequently meeting complicated appendicitis, including...Full Text Available

2011-06-01

358

British Library Electronic Table of Contents (United Kingdom)

Aims A depressed cGMP-dependent protein kinase (PKG) activity is implicated in nitrate tolerance. The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance. Methods and results Nitrate tolerance in in vitro preparations was obtained by a 24 h incubation with nitroglycerin (NTG). Nitrate tolerance in in vivo preparations was obtained by subcutaneous injection of mice with NTG, and the aortas were used. Protein levels of total MYPT1, MYPT1 (LZ+), PP1Cd, myosin light chain (MLC), and phosphorylated MLC were determined by Western blot analysis. Isometric vessel tension was determined by an organ chamber technique. Protein levels of MYPT1...

2010-01-01

359

Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi

Comparative study of functional properties of commercial and membrane processed yellow pea protein isolates

English 21 Jan 2001 [10 p.] United Kingdom Kumaran, D Eswaramoorthy,

2001-01-21

360

British Library Electronic Table of Contents (United Kingdom)

Functional properties of commercial and membrane processed pea protein isolates (PPI) prepared from yellow peas were investigated. Four protein isolates were prepared from yellow pea flour using water and KCl extractions at 25^oC followed by ultrafiltration and diafiltration (UF and DF) at pHs of 7.5 and 7.5 or 6 respectively. Following assessment of compositional attributes; solubility, foaming, flow and dynamic rheology, emulsification ability and heat-induced textural and rheological properties of prepared PPIs and a commercially available PPI were tested and compared. Membrane purification of proteins resulted in 28% to 68% reduction in phytic acid and enhanced, comparatively, the tested functional properties. Solubility of membrane processed PPIs, at all tested pHs, was superior and t...

2011-01-01

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361

Clonidine, octopaminergic receptor agonist, reduces protein feeding in the blow fly, Phormia regina (Meigen)

British Library Electronic Table of Contents (United Kingdom)

Results in this study are consistent with those of Murdock and his colleagues who clearly demonstrated that clonidine, an agonist of octopaminergic receptors in some insects, significantly increases sucrose feeding. Their studies, however, did not examine the effect of clonidine on protein feeding. Injection of a 20mg/ml/fly dose of clonidine significantly reduces protein feeding in both sexes of Phormia regina, instead of stimulating feeding as is observed with carbohydrate feeding. The manner in which the flies are fed prior to starvation and the method of testing influences the amounts of diet consumed. It is proposed that the biogenic amines influence the state of hunger (i.e., protein versus carbohydrates) while other chemicals and neural mechanisms (i.e., such as sulfakinins and stre...

2007-01-01

362

Carbohydrate-specified endocytosis: localization of ligand in the lysosomal compartment.

BzpF is a CREB-like transcription factor that regulates spore maturation and stability in Dictyostelium

Carbohydrate-directed endocytosis is mediated by a receptor, the hepatic binding protein; it is responsible for the clearance of galactose-terminated glycoproteins from the circulation. This process...Full Text Available

1981-11-01

363

British Library Electronic Table of Contents (United Kingdom)

The cAMP response element-binding protein (CREB) is a highly conserved transcription factor that integrates signaling through the cAMP-dependent protein kinase A (PKA) in many eukaryotes. PKA plays a critical role in Dictyostelium development but no CREB homologue has been identified in this system. Here we show that Dictyostelium utilizes a CREB-like protein, BzpF, to integrate PKA signaling during late development. bzpF^- mutants produce compromised spores, which are extremely unstable and germination defective. Previously, we have found that BzpF binds the canonical CRE motif in vitro. In this paper, we determined the DNA binding specificity of BzpF using protein binding microarray (PBM) and showed that the motif with the highest specificity is a CRE-like sequence. BzpF is necessary to ...

2011-01-01

364

Avoidance of hydrolyzed casein by mice

Association and expression study of synapsin III and schizophrenia

When casein, a milk protein, is hydrolyzed, it renders human foods that contain it (e.g., hypoallergenic infant formula, cheeses) distasteful to many people. This rejection...Full Text Available

2008-01-28

365

Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter...Full Text Available

2009-11-20

366

Alpha-toxin of Staphylococcus aureus.

Individual photophysical parameters of the chromophore of a fluorescent protein mRFP1 and its two mutants (amino-acid substitution at position 66 - mRFP1/ Q66C and mRFP1/Q66S proteins) are determined. For this purpose, apart from conventional methods of fluorimetry and spectrophotometry, nonlinear laser fluorimetry is used. It is shown that the individual extinction coefficients of the chromophore of proteins correlate (correlation coefficient above 0.9) with the volume of the substituted amino-acid residue at position 66 (similar to the positions of the absorption, fluorescence excitation and emission maxima). (laser biology and medicine)

2009-03-31

367

Adducted proteins for identification of endogenous electrophiles.

Alpha-toxin, the major cytotoxic agent elaborated by Staphylococcus aureus, was the first bacterial exotoxin to be identified as a pore former. The protein is secreted as a single-chain, water-soluble...Full Text Available

1991-12-01

368

AEC syndrome - Genetics Home Reference

Chemically reactive compounds in tissues can be monitored through their products of reaction with biomacromolecules. For the purpose of in vivo dose monitoring, hemoglobin (Hb) has been preferred to...Full Text Available

1993-03-01

369

A comprehensive assessment of N-terminal signal peptides prediction methods

What genes are related to AEC syndrome? AEC syndrome is caused by mutations in the TP63 gene. This gene provides instructions for making a protein known as p63, which plays an...

2011-10-15

370

0100127 - Liquid nitrogen dewar for protein crystal growth - NASA

BackgroundAmino-terminal signal peptides (SPs) are short regions that guide the targeting of secretory proteins to the correct subcellular compartments in the cell. They are cleaved...Full Text Available

371

The Stellar Populations of Low Luminosity Active Galactic Nuclei. III: Spatially Resolved Spectral Properties

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California, Irvine for use aboard Mir and the International Space ...

372

Structure and kinematics of edge-on galaxy discs -- IV. The kinematics of the stellar discs

In a recent survey of the stellar populations of LINERS and LINER/HII Transition Objects (TOs) we identified a numerous class of nuclei which stand out because of their conspicuous 10^8-9 yr populations. These objects were called ``Young-TOs'', since they all have TO-like emission line ratios. In this paper we investigate the radial variations of spectral properties in Low Luminosity AGN. Our analysis is based on high S/N, 3500-5500 A, long-slit spectra for 47 galaxies. The data probe distances of typically up to 850 pc from the nucleus with a resolution of ~ 100 pc and S/N ~ 30. Stellar population gradients are mapped by the radial profiles of absorption line equivalent widths and colours along the slit. These variations are further analyzed by means of a decomposition of each spectrum in terms of template galaxies representative of very young (<= 10^7 yr), intermediate age (10^8-9 yr) and old (10^10 yr) populations. Our main findings are: ...

2004-01-01

373

Spiral Structures and Shocks in Accretion Discs in Close Binary Systems: the Role of the Stellar Mass Ratio.

The stellar disc kinematics in a sample of fifteen intermediate- to late-type edge-on spiral galaxies are studied using a dynamical modeling technique. The sample covers a substantial range in maximum rotation velocity and deprojected face-on surface brightness and contains seven spirals with either a boxy- or peanut-shaped bulge. Dynamical models of the stellar discs are constructed using the disc structure from $I$-band surface photometry and rotation curves observed in the gas. The differences in the line-of-sight stellar kinematics between the models and absorption line spectroscopy are minimized using a least-squares approach. The modeling constrains the disc surface density and stellar radial velocity dispersion at a fiducial radius through the free parameter $\\sqrt{M/L}$ $(\\sigma_{\\rm z}/\\sigma_{\\rm R})^{-1}$, where $\\sigma_{\\rm z}/\\sigma_{\\rm R}$ is the ratio of vertical and radial velocity dispersion and $M/L$ the disc ...

2005-01-01

374

Some numerical problems in atomic physics

% In this work we investigated, in the Smooth Particle Hydrodynamics (SPH) framework, the development of spiral structures and shock fronts in the radial flow of accretion discs in close binary systems. These shock waves take place when the initially supersonic radial flow penetrating the disc bulk, reduces substantially its speed becoming suddenly subsonic. To this purpose, keeping constant the mass of the compact primary (M1 = 1 MO ), the separation between the two components and the injection speed at the inner Lagrangian point L1 (close to the local sound speed), we carried out 2D SPH simulations for four values of the stellar mass ratio M2/M1. We worked out 2D models because the damping effect of the artificial viscosity is too strong in 3D. Furthermore, the 2D environment seems the most suitable in order to evidence shock fronts in highly compressible gases. The results show that spiral structures and shock fronts develop for low values ...

2000-06-01

375

Sensitivity-based optimal capacitor placement on a radial distribution feeder

The numerical calculation of oscillatory wavefunctions occurring in the theory of electron scattering from positive ions is considered, the aim being to develop methods for evaluating the oscillatory solutions using a logarithmic grid. Thus, the permitted step-size for the solution depends only upon the potential, enabling calculations at different energies to be performed using the same discretisation. Two similar generalisations of the W.K.B. transformation for second order ordinary differential equations are made and applied to the radial Dirac equations. Iterative schemes for the solution of the resulting non-linear amplitude equations are discussed, one for use in the asymptotic region and the other a Newton scheme for continuing the solution closer to the origin. Using these techniques a reliable and efficient program for calculating relativistic scattering phase shifts from ionic potentials has been written. Relativistic Optical Model problems have been ...

1981-01-01

376

Observations of toroidal and poloidal rotation in the high-beta tokamak Torus II

Optimal capacitor placement determines the size, type, and location of capacitors to be installed on a radial distribution feeder that will reduce peak power and energy losses while minimizing the costs of investment and installation of the capacitor banks. This paper describes a sensitivity-based optimal placement of capacitors that employs a new load characterization scheme using a voltage-current-angle-logger. The proposed method allows modeling of loads of different power factors for different portions of the distribution feeder. The optimal solution is obtained by testing various combinations of capacitor banks (based on the smallest bank size specified by the user) and candidate nodes along the distribution feeder, and calculating the resultant savings. In order to reduce solution time, the candidate nodes are ranked according to their sensitivity factors. The highest ranking nodes are considered first in the optimization process. At a node where the ...

1995-12-31

377

Observations of toroidal and poloidal rotation in the high beta tokamak Torus II

The macroscopic rotation of plasma in a toroidal containment device is an important feature of the equilibrium. Toroidal and polidal rotation in the high-beta tokamak Torus II is measured experimentally by examining the Doppler shift of the 4685.75 A He II line emitted from the plasma. The toroidal flow at an average velocity of 1.6 x 10/sup 6/ cm/sec, a small fraction of the ion thermal speed, moves in the same direction as the toroidal-plasma current. The poloidal flow follows the ion diamagnetic current direction, also at an average speed of 1.6 x 10/sup 6/ cm/sec. In view of certain ordering parameters, the toroidal flow is compared with predictions from neoclassical theory in the collisional, Pfirsch-Schluter regime. The poloidal motion, however, results from an E x B drift in a positive radial electric field, approaching a stable ambipolar state. This radial electric field is determined from theory by using the measured poloidal velocity. ...

1983-01-01

378

Nitrogen oxide abatement by distributed fuel addition. Quarterly report No. 8, May 1, 1989--July 31, 1989

The macroscopic rotation of plasma in a toroidal containment device is an important feature of the equilibrium. Toroidal and poloidal rotation in the high beta tokamak Torus II is measured experimentally by examining the Doppler shift of the 4685.75 A He II line emitted from the plasma. The toroidal flow at an average velocity of 1.6 x 10/sup 6/ cm/sec, a small fraction of the ion thermal speed, moves in the same direction as the toroidal plasma current. The poloidal flow follows the ion diamagnetic current direction, also at an average speed of 1.6 x 10/sup 6/ cm/sec. In view of certain ordering parameters, the toroidal flow is compared with predictions from neoclassical theory in the collosional, Pfirsch-Schluter regime. The poloidal motion, however results from an E x B drift in a positive radial electric field, approaching a stable ambipolar state. This radial electric field is determined from theory by using the measured poloidal velocity. ...

1983-01-01

379

Modeling jet penetration in glass

The performance of the new combustor was tested. Experiments showed that this combustor has superior insulating quality than the previous one. Measured radial concentrations indicated uniform distribution of the primary fuel (coal) inside the fire tube resulting in reasonably flat concentration profiles. However, measured radial concentrations after the addition of the reburn fuel showed uneven distribution of the fuel resulting in parabolic concentration profiles, an indication of insufficient mixing in the reburn zone. In order to study mechanisms of nitrogenous species destruction in the reburn zone, it is necessary to achieve adequate mixing if the assumption of one dimensional plug flow behavior is to be valid. Thus, it became necessary to modify the injector design and the injection mode of the reburn fuel to achieve an adequate level of mixing which is the focus of this report. The results show that a minimum reburn fuel jet velocity of ...

1989-08-25

380

Lubrication of an 85-mm ball bearing with RP-1

It is well known that composite armors, consisting of glass elements confined with metallic tampers, are extraordinarily effective against shaped charge attack. Early experiments showed that jets fired against glass targets appeared to be bombarded by tiny fragments that destroyed alignment and reduced residual jet penetration. This result was attributed to an elastic rebound effect in which the outward radial motion of the target around the jet periphery was supposed to be arrested by tensile hoop stresses which developed behind the divergent shock front. Glass fragments were then accelerated radially inward and these destabilized the jet. To sustain the large tensile hoop stresses required, the glass must not have been fractured by the initial shock, behind which the stress state is typically well above the Hugoniot elastic limit. Modeling the penetration of a jet into glass requires not only determining the spall strength, i.e., the tensile ...

1990-04-05

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Investigation of the frequency-dependence of the MTC noise estimator

A parametric experimental investigation of an 85 millimeter bore angular contact ball bearing running in RP-1 fuel was performed at speeds of 10,000 to 24,000 rpm. Thrust loads were varied from 4450 to 17,800 Newtons (1000 to 4000 lbs.). Radial loads were varied from 1335 to 13,350 Newtons (300 to 3000 lbs.). RP-1 lubrication for the bearing was provided through a stationary jet ring located adjacent to the test bearing outer ring. Increases in both the thrust and radial loads resulted in increased bearing temperature, while increases in shaft speed resulted in much more dramatic increases in bearing temperature. These trends are typical for ball bearings operating under these types of conditions. Results are given for outer ring temperatures of the test bearing at the various test conditions employed. In addition, the heat energy removed from the bearing by the RP-1 was determined by measuring the increase in temperature as the RP-1 passed ...

1993-01-01

382

Fuel spray evolution; Comparison of experiment and CFD simulation of nonevaporating spray

The Moderator Temperature Coefficient of reactivity (MTC) was earlier proven to be properly determined via noise analysis when the neutron noise is measured locally and when the radial average of the moderator temperature noise is used. Nevertheless, measurements revealed that the MTC was correctly estimated on a much narrower frequency band (0.6 - 0.7 Hz) than expected (0.1 - 1.0 Hz). The present paper investigates the effect of the feedback on the MTC determination using this new MTC noise estimator (which could explain the deviation at low frequencies, i.e. between 0.1 - 0.5 Hz) and the effect of the detector time constants (which could explain the deviation at high frequencies, i.e. between 0.7 - 1.0 Hz). For that purpose, a coupled neutronic/thermalhydraulic model of the Ringhals-3 PWR is developed in the frequency-domain, based on a radial 2-dimensional heterogeneous representation of the core. The transfer function of the detectors is ...

2005-09-12

383

Experimental study of the characteristics of solidification of stearic acid in an annulus and its thermal conductivity enhancement

Detailed spray characteristics were obtained for a small-capacity, pressure-swirl atomizer using an Aerometrics phase-Doppler particle analyzer. Measurements included drop size and velocity distributions, liquid volume fluxes, and air velocities at four axial locations, 25,50,75, and 100 mm, with complete radial traverses at each location. Drop size results were compared with measurements from a Malvern laser-diffraction instrument, and integrated liquid volume fluxes were compared with measured flow rates to estimate measurement uncertainties. Drop sizes measured by the two independent techniques and area-weighted-averaged over the radial traverses at each of the four axial stations varied on average by less than 4 percent. Integrated volume flux measurements by the phase-Doppler instrument at four axial stations differed from the nozzle flow rate by at most 19 percent, with some of the difference due to evaporation. The phase-Doppler data ...

1989-01-01

384

Experimental study of the characteristics of solidification of stearic acid in an annulus and its thermal conductivity enhancement

The thermal and heat transfer characteristics of stearic acid during the solidification processes were investigated experimentally in a vertical annulus energy storage system. The temperature distribution and temperature variations with time at different radial positions during the freezing processes were obtained. The thermal characteristics of the stearic acid, including movement of the solid-liquid interface in the radial direction, and the effects of Reynolds number on the heat transfer parameters were studied. The heat flux was estimated by using a simple approximate model. A new copper fin was designed and fixed to the electrical heating rod to enhance the thermal conductivity of the stearic acid. The results show that the new fin can enhance both the conduction and the natural convection heat transfer of the PCM, and the enhancement factor during solidification is estimated to be as high as 250%. The effect of the fin width on the ...

2005-04-01

385

Energy-resolved electron particle and energy fluxes in positive column plasmas

The thermal and heat transfer characteristics of stearic acid during the solidification processes were investigated experimentally in a vertical annulus energy storage system. The temperature distribution and temperature variations with time at different radial positions during the freezing processes were obtained. The thermal characteristics of the stearic acid, including movement of the solid-liquid interface in the radial direction, and the effects of Reynolds number on the heat transfer parameters were studied. The heat flux was estimated by using a simple approximate model. A new copper fin was designed and fixed to the electrical heating rod to enhance the thermal conductivity of the stearic acid. The results show that the new fin can enhance both the conduction and the natural convection heat transfer of the PCM, and the enhancement factor during solidification is estimated to be as high as 250%. The effect of the fin width on the ...

2005-04-01

386

Development of magnetic drive packless valves for commercial purpose

This paper deals with electron flux densities and electron energy flux densities in positive column discharges. Recent kinetic calculations by Uhrlandt and Winkler have revealed the interesting physical phenomenon of radially inward directed energy flux densities in positive column plasmas. We have used a self-consistent positive column model, based on an accurate and highly detailed Monte Carlo code, to study this effect in more depth. The results of this study show a rather complex physical picture of electron particle and energy flux densities. Electrons with low energies usually exhibit radially outward directed particle and energy flux densities. At energies above the threshold for electronic excitation particle and energy flux densities are usually inward directed. Only close to the wall, at total energies above the wall potential energy, do these flux densities point towards the wall. The thickness of this 'wall loss ...

1999-11-07

387

Cosmic-Ray Nuclei, Antiprotons and Gamma-rays in the Galaxy: a New Diffusion Model

A study on development of magnetic drive packless valves for commercial purpose showed the results as follows; 1. Study on the radial rays effecting to the permanent magnets -Measurement of the strength of Nd-magnets according to irradiation of radial rays. 2. Effects of temperature on the magnetic driving device -Temperature dependency of the Nd-casting magnets. -Effects of temperature on the heat releasing fins of high-temperature valve. 3. Optimization of torque -Arranging method of permanent magnets -Measuring method and results of torque. 4. Design, manufacture and test for the pressure-resisting structure of magnetic power transmitting device -Calculation and design for the flat circular plates under pressure of the magnetic power transmitting device -Design, manufacture and test for the pressure-resisting structure of magnetic power transmitting device -Comparison of the characteristics between magnetic drive valve and ...

1995-09-01

388

A numerical investigation of the aerodynamics of a furnace with a movable block burner

We model the transport of cosmic ray nuclei in the Galaxy by means of a new numerical code. Differently from previous numerical models we account for a generic spatial distribution of the diffusion coefficient. We found that in the case of radially uniform diffusion, the main secondary/primary ratios (B/C, N/O and sub-Fe/Fe) and the modulated antiproton spectrum match consistently the available observations. Convection and re-acceleration do not seem to be required in the energy range we consider: $1 \\le E \\le 10^3$ GeV/nucleon. We generalize these results accounting for radial dependence of the diffusion coefficient, which is assumed to trace that of supernova remnants. While this does not affect the prediction of secondary/primary ratios, the simulated longitude profile of the diffuse $\\gamma$-ray emission is significantly different from the uniform case and may agree with EGRET measurements without invoking {\\it ad hoc} assumptions on ...

2008-01-01

389

Scientific Electronic Library Online (English)

Abstract in english In this work the air flow in a furnace was computationally investigated. The furnace, for which experimental test data are available, is composed of a movable block burner connected to a cylindrical combustion chamber by a conical quarl. The apertures between the movable and the fixed blocks of the burner determine the ratio of the tangential to the radial air streams supplied to the furnace. Three different positions of the movable blocks were studied at this time. A thr (more) ee-dimensional investigation was performed by means of the finite volume method. The numerical grid was developed by the multiblock technique. The turbulence phenomenon was addressed by the RNG k-epsilon model. Profiles of the axial, tangential and radial velocities in the combustion chamber were outlined. The map of the predicted axial velocity in the combustion chamber was compared with a map of the experimental axial velocity. The internal space ...

2007-06-01

390

Topological Defects in the Moduli Sector of String Theory

The utility of the SH01-195 spindle deflector

We point out that the moduli sector of the $(2,2)$ string compactification with its nonperturbatively preserved non-compact symmetries is a fertile framework to study global topological defects, thus providing a natural source for the large scale structure formation. Based on the target space modular invariance of the nonperturbative superpotential of the four-dimensional N=1 supersymmetric string vacua, topologically stable stringy domain walls are found. They are supersymmetric solutions, thus saturating the Bogomolnyi bound. It is also shown that there are moduli sectors that allow for the global monopole-type and texture-type configurations whose radial stability is ensured by higher derivative terms.

1991-01-01

391

Static and dynamic muonic-atom codes-MUON and RURP

The All Union Scientific Research Institute of Drilling Techniques has developed the SH01-195 spindle deflector designed for use with the 195 millimeter turbodrill sections in place of the standard spindle. This spindle deflector is an axial support for the turbodrill, manufactured as a separate unit that attaches to the turbosections using the standard attachment configuration for series manufactured turbodrills, using the sub for attachment to the frame and a splined half coupling for attachment to the shaft. The spindle deflector itself consists of two parts connected by a curved sub through the frame and by a double hinge to the shaft. The hinge is isolated from axial forces by mounting radial support ball bearings above and below it.

1984-01-01

392

Spatial and angular characteristics of microsecond negative-ion beams in a magnetically insulated diode

Muonic-atom energy levels and wave functions are calculated. The results are corrected for nearly all important static effects, including finite nuclear size as produced by a user-specified nuclear charge distribution. Fourth-order Runge-Kutta integration of the radial Dirac equations, with the secant method determining the eigenvalues. Corrections are either included as potentials in the Dirac equations or computed as first-order perturbations. The code assumes spherical symmetry, so that effects of nuclear deformation may be treated only as static first-order perturbations. Dynamic muon-electron and muon-nuclear effects are neglected (the latter are calculated by RURP). (Auth.).

1979-02-01

393

Regeneration behavior of light-duty catalytic trap oxidizer systems

This paper describes the design of a high-voltage negative ion source based on a magnetically insulated diode and generating microsecond pulses. Plane an cylindrical cathodes have been tested. The spatial and angular distributions of negative ions in the beam have been measured. The content of negative ions with different masses in the beam are given. The ion current density measured by a Faraday cup was up to 1 A/cm{sup 2} for the radial beam and 30-40 A/cm{sup 2}.

1995-10-01

394

Proceedings of the first analysis meeting on JUPITER-II Program

A catalytic trap oxidizer based on a radial flow metal mesh filter was developed for use as an after treatment device on light-duty diesel vehicles. A complete system for assisted regeneration was devised to enable this unit to operate without blocking during all normal driving modes. The integration of the catalyzed trap and regeneration system with the vehicle to provide a practical system for the reduction of diesel particulate emissions is discussed. To date, many prototype catalytic trap and regeneration system installations have been completed on various test vehicles, both naturally aspirated and turbocharged. The operational conditions and test results from a selection of these studies are presented.

1984-01-01

395

Orbits of Four Very Massive Binaries in the R136 Cluster

The JUPITER-II Program is the Joint Physics Large Heterogeneous Core Critical Experiments Program between the U.S. Department of Energy (US DOE) and PNC, Japan. The experiments began in May 1982 and ended in April 1984, as a part of the ZPPR-13 program. The ZPPR-13 is a series of critical assemblies designed to study the fundamental neutronic behavior of large, radially-heterogeneous LMFBR cores. This report describes the results of analysis of ZPPR-13A and preliminary analysis of ZPPR-13B, and some topics of recent activities in fast reactor physics.

1984-12-31

396

One-particle characteristics in problems with allowance for complex configurations

We present radial velocity and photometry for four early-type, massive double-lined spectroscopic binaries in the R136 cluster. Three of these systems are eclipsing, allowing orbital inclinations to be determined. One of these systems, R136-38 (O3 V + O6 V), has one of the highest masses ever measured, 57 Mo, for the primary. Comparison of our masses with those derived from standard evolutionary tracks shows excellent agreement. We also identify five other light variables in the R136 cluster which are worthy of follow-up study.

2001-01-01

397

Noncircular gas velocities and the radial dependence of mass-to-light ratio in NGC 4594 (the Sombrero Galaxy)

A method for the determination of different ''bare'' characteristics of the one-particle motion and one-particle basis in magic nuclei is described. The method is based on separating out the mixing with phonons from the phenomenological one-particle characteristics. By means of a generalization of the procedure for localization of the mass operator, relations linking the bare and phenomenological characteristics are obtained. The radial dependence of these characteristics in finite nuclei and the influence of the quasiparticle--phonon interaction on the phenomenological characteristics are studied. Calculations are performed for the neutrons in /sup 208/Pb.

1986-09-01

398

Magnetic fluctuation measurement in Sino United Spherical Tokamak plasma

The mass distribution of the Sombrero Galaxy, NGC 4594, is calculated in order to investigate the suspicion that the rotational velocity of the galactic gas does not measure the circular velocity in the galaxy. It is shown that the H II rotation velocities are much less than circular in the central 35 arcsec of the galaxy, and that the suspicion is correct. Thus, the H II rotation velocities cannot be used to measure the mass distribution. The absorption-line rotation curve is used to derive the mass distribution, and it is found that the M/L ratio is nearly constant. It is concluded that the visible matter is self-gravitating at least in the central 180 arcsec. 44 references.

399

Magnetic braking in weakly ionized media

To investigate the magnetic fluctuations and for further transport study, the poloidal and radial magnetic field measurement is conducted on the Sino United Spherical Tokamak (SUNIST). Auto-power spectral density indicates that the magnetic fluctuation energy mainly concentrates in the frequency region lower than 10 kHz. The magnetic field oscillations, which are characterized by harmonic frequencies of 40 kHz, are observed in the scrape-off layer; by contrast, in the plasma core, the magnetic fluctuations are of Gaussian type. The time-frequency profiles show that the poloidal magnetic fluctuations are temporally intermittent. The autocorrelation calculation indicates that the fluctuations in decorrelation time vary between the core and the edge. (authors)

2007-07-01

400

Longitudinal emittance oscillation in a superconducting drift tube linac

The combined magnetic braking-ambipolar diffusion problem in weakly ionized, rigidly rotating disks is studied. An analytical solution is presented for a disk whose angular velocity and magnetic yield vectors are aligned with the symmetry axis, illustrating the effects of the relative azimuthal drift of neutrals and ions. The effects of radial drift are added, commenting on the ratio of the characteristic ambipolar diffusion and magnetic braking time scales in high-mass and low-mass disks. A numerical calculation is used to show the combined action of these two processes. 31 references.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Exact solutions to the Chandrasekhar Page angular equation

In drift tube linacs a beam energy spread results form the finite beam size. Radial variation of the axial accelerating field induces a beam energy spread, which, in general, will accumulate as the beam passes through successive drift tubes. This paper shows that under some conditions of periodic transverse focusing and longitudinal phase focusing, the correlation between the longitudinal and transverse motion can be used to correct the energy spread. The process of achieving such a correction is first described in a simplified situation, and then demonstrated for a particular tuning using a ray-tracing program which models a low velocity and low charge state linac designed for radioactive ion beams.

1995-05-01

402

Estimation of static formation temperatures in geothermal wells

Exact solutions are found for the Chandrasekhar Page angular equation which results when the Dirac equation in a Kerr Newman space time is separated into its radial and angular parts. The solutions turn out to be remarkably simple in form while satisfying the asymptotic conditions deduced earlier. The eigenvalues are found to be the square root of the total angular momentum as first found by Dirac for flat space; supplemented by a term which is the product of the mass of the Dirac particle times the specific angular momentum of the black hole. The additional contribution is what is expected from frame dragging.

2006-01-01

403

Electrical properties of airborne nanoparticles produced by a commercial spark-discharge generator

Stabilized formation temperatures were estimated at different depths in 40 wells from the Los Humeros geothermal field, Mexico, using the Horner and the spherical radial flow (SRF) methods. The results showed that the Horner method underestimates formation temperatures, while the SRF method gives temperatures that are closer to the true formation temperatures. This was supported by numerical simulation of a combined circulation and shut-in period in several wells, and results for well H-26 are presented. Numerical reproduction of logged temperature is more feasible if an initial temperature profile based on the SRF method is employed instead of using an initial temperature profile based on the Horner method. (Author)

2003-05-01

404

Dirac equation on a G_2 manifold

A nanoparticle generator based on the principle of electrical discharge (PALAS GFG-1000) was used to produce nanoparticles of different chemical natures. The fractions of electrically neutral particles were then measured by means of a Spectrometre de Mobilite Electrique Circulaire (SMEC, i.e. radial-flow mobility analyzer) for different operating conditions. The experimental results were compared with the theoretical values calculated from the Fuchs extended charge equilibrium model for spherical particles and agglomerates. For the smallest particles (below 20 nm), the deviations observed remain below 10%, and tend towards 20% for larger particles (over 35 nm).

2010-08-01

405

Core localized toroidal Alfven eigenmodes destabilized by energetic ions in the CHS heliotron/torsatron

We find a large family of solutions to the Dirac equation on a manifold of $G_2$ holonomy asymptotic to a cone over $S^3 \\times S^3$, including all radial solutions. The behaviour of these solutions is studied as the manifold developes a conical singularity. None of the solutions found are both localised and square integrable at the origin. This result suggests that M-theory on the conifold over $S^3\\times S^3$ does not produce a chiral theory. The approach here is complementary to previous analyses using dualities and anomaly cancellation which found chiral fermions on all known $G_2$ conifolds except that over $S^3\\times S^3$.

2002-01-01

406

Collisional transport in a plasma with steep gradients

Toroidal Alfven eigenmodes (TAE) destabilized by the pressure gradient of energetic alpha particles may expel the alpha particles before thermalization. TAE is important for tokamaks, and for helical systems (stellarators) as well. In CHS (compact helical system) TAE localized in the plasma core are destabilized when the plasma current is induced by co-injection of neutral beams. The observed TAE exhibits a ballooning nature. The internal structure of TAE was measured with a soft X-ray detector. The soft X-ray fluctuations level for TAE is too low to obtain the radial profiles of fluctuation intensities. (Tanaka, M.)

1999-09-01

407

Band structure and electron-electron interaction in samarium monosulphide

The validity is given to the newly proposed two {delta}f method for neoclassical transport calculation, which can be solve the drift kinetic equation considering effects of steep plasma gradients, large radial electric field, finite banana width, and an orbit topology near the axis. The new method is applied to the study of ion transport with steep plasma gradients. It is found that the ion thermal diffusivity decreases as the scale length of density gradient decreases, while the ion particle flux due to ion-ion self collisions increases with increasing gradient. (author)

1999-06-01

408

Application of a 3-beam #gamma# densitometer to two-phase flow regime and density measurements

The method of augmented plane wave (APW) is used to obtain the band structure of the SmS compound in the semiconductor and metal phases. The noncentral part of the Coulomb electron-electron interaction is taken into account in the first order perturbation theory. In this case the radial part of the wave APW-function is taken as a zero approximation function. A multiplet structure of the excited configuration f"5d, which provides a good description of the X-ray photoelectron spectrum and optical spectrum epsilon_2(#omega#), is obtained. The configuration fd is calculated for the interpretation of the optical absorption spectrum of the samarium monosulfide metal phase. (author).

409

A new reconfiguration scheme for voltage stability enhancement of radial distribution systems

A method of using gamma radiation to determine the density and phase distribution in two-phase flows in pipes is described. Three collimated beams of radiation that pass through a pipe cross-section at different radial positions are used. A theory and computer program used to relate the measured attenuation of these beams to a three-parameter model of the phase distribution and to the average density and void fraction are discussed. Data obtained during both static and dynamic verification experiments using Lucite inserts are presented, as well as the results of several tests done in high pressure, steam-water flows.

1976-08-11

410

A high stopping power, large acceptance Bragg curve spectrometer for heavy ion detection

Network reconfiguration is an operation problem, which entails altering the topological structure of the distribution feeders by rearranging the status of switches in order to obtain an optimal configuration in order to minimise the system losses. This paper presents a new reconfiguration algorithm that enhances voltage stability and improves the voltage profile besides minimising losses without incurring any additional cost for installation of capacitors, tap changing transformers and related switching equipment in the distribution system. Test results on a 69 node distribution system reveal the superiority of this algorithm.

2009-09-01

411

A high stopping power, large acceptance Bragg curve spectrometer for heavy ion detection

A large acceptance, high stopping power Bragg curve spectrometer has been developed for use in coincidence experiments with heavy ions. The electron collection fields are radial and position information is obtained from a resistive anode. The detector is 60 cm deep and operates at pressures of up to 2.5 atm of P-10 gas. It is mated to a scattering chamber which allows it to be moved out of plane during coincidence measurements. Factors affecting the performance of the detector are discussed, and results obtained with the detector are presented. (orig.).

1991-05-01

412

Water structure as a function of temperature from X-ray scatteringexperiments and ab initio molecular dynamics

A large acceptance, high stopping power Bragg curve spectrometer has been developed for use in coincidence experiments with heavy ions. The electron collection fields are radial and position information is obtained from a resistive anode. The detector is 60 cm deep and operates at pressures of up to 2.5 atm of P-10 gas. It is mated to a scattering chamber which allows it to be moved out of plane during coincidence measurements. Factors affecting the performance of the detector are discussed, and results obtained with the detector are presented. (orig.).

413

Transmutation of technetium in the Petten HFR. A comparison of measurements and calculations

We present high-quality X-ray scattering experiments on pure water taken over a temperature range of 2 to 77 C using a synchrotron beam line at the advanced light source (ALS) at Lawrence Berkeley National Laboratory. The ALS X-ray scattering intensities are qualitatively different in trend of maximum intensity over this temperature range compared to older X-ray experiments. While the common procedure is to report both the intensity curve and radial distribution function(s), the proper extraction of the real-space pair correlation functions from the experimental scattering is very difficult due to uncertainty introduced in the experimental corrections, the proper weighting of OO, OH, and HH contributions, and numerical problems of Fourier transforming truncated data in Q-space. Instead, we consider the direct calculation of X-ray scattering spectra using electron densities derived from density functional theory based on real-space configurations generated with ...

2003-03-01

414

Radial dose distribution of 6.0 MeV/n #alpha#-particle in water

Within the framework of the EFTTRA cooperation between CEA, ECN, EDF, FZK, IAM and ITU, six metallic {sup 99}Tc rods have been irradiated in the Petten HFR for 193 effective full power days. During this irradiation, more than 6% of the {sup 99}Tc has been transmuted to the stable {sup 100}Ru. At ECN, one of the six rods has been examined in the hot cell laboratory. The ruthenium concentration in the rod measured by Isotope Dilution Mass Spectrometry reaches 6.4% at 5 mm from the bottom of the rod and 6.0% at 5 mm from the top. Also the axial and radial distributions of the ruthenium have been measured by Electron Probe Micro Analysis. The ruthenium concentrations calculated by the three-dimensional Monte Carlo code KENO reach 6.1% at 5 mm from the bottom of the rod and 5.7% at 5 mm from the top. These values are in reasonable agreement with the measured ones. However, the calculated radial distribution of the ruthenium concentration is not in ...

1996-10-01

415

Particle simulation of edge pedestal formation and plasma rotation dynamics

For the study of radiation biology and its application to radiotherapy, the double differential cross section of electron emission from water vapor induced by 6.0 MeV alpha particle beam is measured. The energy spectra of electrons ranging 7- 10000 eV are detected by the electrostatic analyzer and micro channel plate. The measurements are made at angles between 20 and 160 degrees. With use of this data set, the radial dose distribution in water is calculated by using KURBUC code. It is the Monte Carlo type code of the electron transport process, where the track of the electron is simulated through each individual interactions including elastic scattering, ionization cross section and total excitation cross section in case that electrons with certain energy are put in the liquid-density water. In order to understand the effect of radiation when the particle flux is injected in the human body like radiotherapy using accelerator beam, the dose distribution in the ...

2003-08-17

416

Continuum damping of toroidal Alfven eigenmodes in finite-#beta# tokamak equilibria

Gyrokinetic particle simulation of edge pedestal formation and plasma rotation dynamics will be presented, and compared with experimental observations. Realistic tokamak edge geometry is used which include separatrix/X-point and material wall from EFIT g-eqdsk data. In order to handle adequately the spatially inhomogeneous electric potential in the scrape-off region, the full-f electron technique is used, in addition to the full-f ions. Monte Carlo neutral particles with wall recycling coefficient will be included self-consistently with the plasma kinetics. Ion-ion Coulomb collisions will be particle, momentum and energy conserving. Energy source for the pedestal and scrape-off plasmas is the heat flow from the core plasma, and the particle source is the ionization of the neutral atoms which are either wall recycled and/or gas puffed. The simulation will be self-consistent with the first principles nonlinear neoclassical and (electrostatic so far) turbulence interactions. Plasma ions ...

2007-03-26

417

Anatomical changes after radical surgery and reconstruction with pedunculated or revascularized flaps in advanced head and neck cancer: CT and MR features; Modificazioni anatomiche dopo chirurgia radicale e ricostruzione con lembi peduncolati o rivascolarizzati nei tumori cervico-facciali negli stadi avanzati: Aspetti con Tomografia Computerizzata e Risonanza Magnetica

A general theoretical approach for the study of the two-dimensional structure of high-n Toroidal Alfven Eigenmodes (TAE) in finite-#beta#, large aspect ratio (R_o/a much-gt 1) tokamak equilibria is presented. Here, n is the toroidal mode number, #beta# = plasma/magnetic pressure, and a(R_o) is the minor (major) radius of the torus. It is shown how the general pseudo-differential boundary value problem for the radial eigenmode structure can be systematically constructed from the local dispersion relation; which is obtained using the ballooning formalism. The TAE modes are characterized by a broad radial envelope, the width of which is independent on the mode number in the general case of monotonic equilibrium profiles. The results on the two-dimensional eigenmode structure are expected to be applicable to drift-type waves. The ballooning transform is generalized here to handle singular eigenfunctions typical of the continuous shear Alfven ...

418

Anatomical changes after radical surgery and reconstruction with pedunculated or revascularized flaps in advanced head and neck cancer: CT and MR features

January, 1992, to October, 1995, sixty-four patients with advanced head and neck cancer underwent head and neck reconstructive surgery using myocutaneous or revascularized flaps; in the same period, all patients were consecutively examined with CT and MRI. Myocutaneous flaps were used in 26 patients: 12 flaps were tubular and 14 linear. Revascularized flaps were used in 38 patients: to repair a large defect in 26 patients (14 latissimus dorsi flaps and 12 temporal muscle flaps) and to repair an oral damage in 12 patients (5 revascularized radial and 7 jejunal flaps). CT and MR images of myocutaneous flaps showed the flaps as fatty areas, repairing large surgical defects, hypodense at CT and hyperintense at MRI, with no post-contrast enhancement. The appearance of revascularized flaps at CT and MRI depends on the characteristics of the structure used to repair the surgical defect: jejunal and radial flaps appeared as mostly fatty thickened ...

1997-04-01

419

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits.

January, 1992, to October, 1995, sixty-four patients with advanced head and neck cancer underwent head and neck reconstructive surgery using myocutaneous or revascularized flaps; in the same period, all patients were consecutively examined with CT and MRI. Myocutaneous flaps were used in 26 patients: 12 flaps were tubular and 14 linear. Revascularized flaps were used in 38 patients: to repair a large defect in 26 patients (14 latissimus dorsi flaps and 12 temporal muscle flaps) and to repair an oral damage in 12 patients (5 revascularized radial and 7 jejunal flaps). CT and MR images of myocutaneous flaps showed the flaps as fatty areas, repairing large surgical defects, hypodense at CT and hyperintense at MRI, with no post-contrast enhancement. The appearance of revascularized flaps at CT and MRI depends on the characteristics of the structure used to repair the surgical defect: jejunal and radial flaps appeared as mostly fatty thickened ...

1997-01-01

420

Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung. Relationship between uteroglobin and eicosanoid levels in the developing fetal lung.

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the...Full Text Available

1993-05-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

The molybdenum iron-sulphur protein from Desulfovibrio gigas as a form of aldehyde oxidase.

Uteroglobin (UG) gene encodes a cytokine-like, multifunctional, antiinflammatory protein, with potent phospholipase A2-inhibitory activity. It has been suggested that during implantation this protein...Full Text Available

1995-07-01

422

The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

The molybdenum iron-sulphur protein originally isolated from Desulfovibrio gigas by Moura, Xavier, Bruschi, Le Gall, Hall & Cammack [(1976) Biochem. Biophys. Res. Commun. 72, 782-789] has been...Full Text Available

1987-05-01

423

The holographic principle and the language of genes

We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb...Full Text Available

1987-12-23

424

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

We show that the holographic principle in quantum gravity imposes a strong constraint on life. The degrees of freedom of an organism can be estimated according to the theory of Boolean networks, which is constrained by the entropy bound. Hence we can explain the languages in protein sequences or in DNA sequences. The overall evolution of biological complexity can be illustrated. And some general properties of protein length distributions can be explained by a linguistic mechanism.

2008-01-01

425

The Membrane Bound LRR Lipoprotein Slr, and the Cell Wall-Anchored M1 Protein from Streptococcus pyogenes Both Interact with Type I Collagen

The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in...Full Text Available

2004-07-01

426

The Function of Hypoxia-Inducible Factor (HIF) Is Independent of the Endoplasmic Reticulum Protein OS-9

Streptococcus pyogenes is an important human pathogen and surface structures allow it to adhere to, colonize and invade the human host. Proteins containing leucine rich repeats (LRR)...Full Text Available

427

Temporal patterns of protein phosphorylation after angiotensin II, A23187 and/or 12-O-tetradecanoylphorbol 13-acetate in adrenal glomerulosa cells.

The protein “amplified in osteosarcoma-9” (OS-9) has been shown previously to interact with the prolyl hydroxylases PHD2 and PHD3. These enzymes initiate oxygen-dependent degradation...Full Text Available

428

Synthesis and cloning of the genes of antisense peptides of human calcitonin and miniproinsulin

The temporal patterns of protein phosphorylation in the adrenal glomerulosa cell were analysed by two-dimensional electrophoresis after stimulation with 10 nM-angiotensin II or various agents [10 nM-12-O-tetradecanoylphorbol...Full Text Available

1986-09-15

429

Structural features underlying selective inhibition of protein kinase CK2 by ATP site-directed tetrabromo-2-benzotriazole

With the aim of an experimental check on the validity of the theory of molecular recognition, the authors have carried out the chemical-enzymatic synthesis and cloning of the gene of human calcitonin and also of the genes of antisense polypeptides to human calcitonin and miniproinsulin. It has been shown that recombinant plasmids obtained on the basis of these synthetic genes are capable of ensuring the biosynthesis of the given polypeptides in E. coli cells as hybrid proteins with the IgG-binding domain of staphylococcal protein A.

1994-07-20

430

Structural Chemistry of Human SET Domain Protein Methyltransferases

Two novel crystal structures of Zea mays protein kinase CK2α catalytic subunit, one in complex with the specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) and another...Full Text Available

2001-11-01

431

Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

There are about fifty SET domain protein methyltransferases (PMTs) in the human genome, that transfer a methyl group from S-adenosyl-L-methionine (SAM) to substrate lysines on histone tails or other peptides. A number of structures in complex with cofactor, substrate, or inhibitors revealed the mechanisms of substrate recognition, methylation state specificity, and chemical inhibition. Based on these structures, we review the structural chemistry of SET domain PMTs, and propose general concepts towards the development of selective inhibitors.

2011-08-22

432

SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

BackgroundLand plant genomes contain multiple members of a eukaryote-specific gene family encoding proteins with pentatricopeptide repeat (PPR) motifs. Some PPR proteins were shown...Full Text Available

433

Roles of the TNF receptor-associated factor (TRAF)2/3 binding site in differential B cell signaling by CD40 and its viral oncogenic mimic, latent membrane protein 1 (LMP1)

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

434

Repetitive satellite-like sequences are present within or upstream from 3 avian protein-coding genes.

The Epstein-Barr virus protein, LMP1, is a functional mimic of the cellular receptor CD40, but signals to B lymphocytes in an amplified and sustained manner compared to CD40. LMP1 contributes...Full Text Available

2009-09-01

435

Relative Rates of Transcapillary Movement of Free Thyroxine, Protein-Bound Thyroxine, Thyroxine-Binding Proteins, and Albumin

Peculiar DNA sequences made up by the tandem repetition of a 5 bp unit have been identified within or upstream from three avian protein-coding genes. One sequence is located within an intron of the...Full Text Available

1983-03-11

436

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation¹^[W]^[OA]

The rate of appearance of labeled thyroxine (T4) and albumin in lymph from various areas after simultaneous i.v. injection of the labeled substances in conscious ambulatory sheep has been used to estimate...Full Text Available

1974-07-01

437

RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12.

Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in storage organelles in the endosperm during seed maturation as a nitrogen source for germinating seedlings....Full Text Available

2010-12-01

438

Protein oxidation markers in women with and without gestational diabetes mellitus: A possible relation with paraoxonase activity.

Escherichia coli rnh mutants lacking RNase H activity are capable of recA+-dependent DNA replication in the absence of concomitant protein synthesis (stable DNA replication). In rnh dnaA::Tn10 and rnh...Full Text Available

1985-08-01

439

Protein dynamics. Vibrational coupling, spectral broadening mechanisms, and anharmonicity effects in carbonmonoxy heme proteins studied by the temperature dependence of the Soret band lineshape.

AIMS: To clarify the levels of protein oxidation markers such as protein carbonyl (PCO), protein hydroperoxides (P-OOH), advanced oxidation protein products (AOPP) and nitrotyrosine (NT), as well as antioxidative enzymes such as paraoxonase (PON-1) in women with and without gestational diabetes mellitus (GDM). METHODS: The study was conducted on 23 women with GDM and 22 women without GDM. The levels of the P-OOH, AOPP, and PON-1 were determined by colorimetric methods; whereas NT and PCO levels were measured by ELISA. RESULTS: The concentrations of protein oxidation markers were significantly increased and PON1 activity was significantly decreased in GDM group compared to those of normal pregnant women. The control group showed a significant negative correlation between PON-1 and PCO (r=-0.451, p=0.027); whereas in GDM group, there was a significant positive correlation between ...

2011-08-30

440

Primary structure of streptococcal Pep M5 protein: Absence of extensive sequence repeats

In this work we study the temperature dependence of the Soret band lineshape of the carbonmonoxy derivatives of sperm whale myoglobin, human hemoglobin, and its isolated alpha and beta subunits. To...Full Text Available

1992-08-01

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441

Position-Dependent Electrostatic Protection against Protein Aggregation

Extensive sequence repeats have been observed in a biologically active fragment of type 24 streptococcal M protein, namely Pep M24 [Beachey, E. H., Sayer, J. M. & Kang, A. H. (1978) Proc....Full Text Available

1983-09-01

442

British Library Electronic Table of Contents (United Kingdom)

Proteins with a high propensity to aggregate can be largely prevented from doing so with surprisingly small changes to their primary structure. By using a combination of rational design and quantitative measurements of aggregation rates, we show that adding a single charge in specific "gatekeeper" regions is sufficient to change the timescale for amyloid fibril growth from minutes to weeks, thereby dramatically reducing the efficiency of this process.

2009-01-01

443

Polyunsaturated Fatty Acids Selectively Suppress Sterol Regulatory Element-binding Protein-1 through Proteolytic Processing and Autoloop Regulatory Circuit*

Phosphorylation of ?6-Tubulin by Protein Kinase C? Activates Motility of Human Breast Cells*

Sterol regulatory element-binding protein (SREBP)-1 is a key transcription factor for the regulation of lipogenic enzyme genes in the liver. Polyunsaturated fatty acids (PUFA) selectively suppress hepatic...Full Text Available

2010-04-09

444

Outcome predictability of biomarkers of protein-energy wasting and inflammation in moderate and advanced chronic kidney disease¹²³⁴

Engineered overexpression of protein kinase Cα (PKCα) was previously shown to endow nonmotile MCF-10A human breast cells with aggressive motility. A traceable mutant of PKCα...Full Text Available

2009-06-26

445

On the Origins of the Weak Folding Cooperativity of a Designed ??? Ultrafast Protein FSD-1

Background: Markers of protein-energy wasting (PEW) and inflammation are common in chronic kidney disease (CKD) and are among the strongest predictors of mortality in dialysis patients.Objective:...Full Text Available

2009-08-01

446

FSD-1, a designed small ultrafast folder with a ββα fold, has been actively studied in the last few years as a model system for studying protein folding mechanisms and for testing...Full Text Available

2010-11-01

447

Mutations Affecting Motifs of Unknown Function in the Central Domain of Nitrogen Regulatory Protein C

BackgroundUsing a transcriptional profiling approach, we recently identified myeloid-related protein-8/14 (MRP-8/14) to be expressed by platelets during acute MI....Full Text Available

2008-01-01

448

Mice With a Deletion in the Gene for CCAAT/Enhancer-Binding Protein ? are Protected Against Diet-Induced Obesity

The positive control function of the bacterial enhancer-binding protein NtrC resides in its central domain, which is highly conserved among activators of ς⁵⁴ holoenzyme. Previous...Full Text Available

1999-09-01

449

Method for detecting and diagnosing disease caused by pathological protein aggregation

The CCAAT/enhancer-binding protein β (C/EBPβ) is required for adipocyte differentiation and maturation. We have studied the role of the transcription factor, C/EBPβ,...Full Text Available

2007-01-01

450

Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

A method is provided for detecting pathological macromolecules in a patient. The method is comprised of the following: obtaining body fluid from the patient; pretreating the body fluid; subjecting the pretreated body fluid to size-exclusion chromatography to create an excluded fluid; and analyzing the excluded fluid to detect macromolecules having a predetermined molecular weight. The method also allows for comparing elution spectra with reference spectra of suspect pathologic proteins.

2000-05-16

451

Involvement of stress-activated protein kinase and p38 mitogen-activated protein kinase in mIgM-induced apoptosis of human B lymphocytes

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available

1996-12-02

452

Immunohistochemical localization of low density lipoprotein receptor-related protein 1 and ?₂-Macroglobulin in retinal and choroidal tissue of proliferative retinopathies

Despite intensive efforts, the intracellular signaling pathways that mediate apoptosis remain unclear. The human B lymphoma cell line, B104, possesses characteristics that make it an attractive model...Full Text Available

1996-11-26

453

Hyperphosphorylation of the Rotavirus NSP5 Protein Is Independent of Serine 67 or NSP2, and the Intrinsic Insolubility of NSP5 Is Regulated by Cellular Phosphatases

The immunolocalization of the low density lipoprotein receptor-related protein 1 (LRP1) and its ligand alpha 2-Macroglobulin (α₂M) was examined in tissues from human donor...Full Text Available

2010-08-01

454

HupUV proteins of Rhodobacter capsulatus can bind H2: evidence from the H-D exchange reaction.

The NSP5 protein is required for viroplasm formation during rotavirus infection and is hyperphosphorylated into 32- to 35-kDa isoforms. Earlier studies reported that NSP5 is not hyperphosphorylated...Full Text Available

2006-02-01

455

High throughput protein fold identification by using experimental constraints derived from intramolecular cross-links and mass spectrometry

The H-D exchange reaction has been measured with the D2-H2O system, for Rhodobacter capsulatus JP91, which lacks the hupSL-encoded hydrogenase, and R. capsulatus BSE16, which lacks the HupUV proteins....Full Text Available

1997-01-01

456

Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase ?

We have used intramolecular cross-linking, MS, and sequence threading to rapidly identify the fold of a model protein, bovine basic fibroblast growth factor (FGF)-2. Its tertiary structure was probed...Full Text Available

2000-05-23

457

Helper T-Cell Epitopes Encoded by the Babesia bigemina rap-1 Gene Family in the Constant and Variant Domains Are Conserved among Parasite Strains

Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), the viral RNA-dependent RNA polymerase (RdRp), is a tail-anchored protein with a highly conserved C-terminal transmembrane domain (TMD) that...Full Text Available

2010-11-01

458

External reflection absorption infrared spectroscopy study of lung surfactant proteins SP-B and SP-C in phospholipid monolayers at the air/water interface.

Among important candidates for babesial vaccines are apical complex proteins, including rhoptry-associated protein 1 (RAP-1) from Babesia bovis and B. bigemina, which...Full Text Available

1998-04-01

459

Exploiting the beneficial effects of PPO on the utilisation of protein and lipids in grazed forages

The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface...Full Text Available

1995-12-01

460

Environmental Research Database

DescriptionThe plant enzyme polyphenol oxidase (PPO) has been demonstrated to reduce both proteolysis and lipolysis during the ensiling of red clover. The purpose of this work is to explore the potential for exploiting this beneficial trait in other dietary regimes for grazing ruminants. These studies will focus on the development of new strategies for forage management and livestock feeding by increasing the efficiency of feed protein-N utilisation and reducing saturated fatty acid production by ruminan [continued...

2007-01-31

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Evaporator condensates: continuous bioprocessing of simulated sulfite condensates to produce single cell protein. [Candida utilis

Ebola Virus VP40-Induced Particle Formation and Association with the Lipid Bilayer

The continuous bioprocessing of simulated steam-stripped sulfite pulp mill evaporator condensates using Candida utilis to produce single cell protein has been studied. At 32 degrees C, processing can be conducted at space velocities of up to 0.43 hr/sup -1/ with over 90% removal of acetic acid present at an initial concentration of 2 g/liter. (Refs. 16).

1982-11-01

462

Differential Changes in Heat Shock Protein-, Lipoarabinomannan-, and Purified Protein Derivative-Specific Immunoglobulin G1 and G2 Isotype Responses during Bovine Mycobacterium avium subsp. paratuberculosis Infection

Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle. To elucidate the functions of VP40, we investigated...Full Text Available

2001-06-01

463

Determination by enzyme-linked immunosorbent assay of immunoglobulin G (IgG), IgM, and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis.

Bovine paratuberculosis is caused by infection of young calves with Mycobacterium avium subsp. paratuberculosis. In some of the chronically infected cows the long asymptomatic...Full Text Available

2001-03-01

464

Decreased serum protein binding of diazepam and its major metabolite in the neonate during the first postnatal week relate to increased free fatty acid levels.

An enzyme-linked immunosorbent assay was used to compare Brucella melitensis major outer membrane proteins (MOMP) and whole-cell heat-killed antigens (HK) in measuring antibrucella immunoglobulin G...Full Text Available

1989-08-01

465

Decreased binding of drugs and dyes to plasma proteins from rats with acute renal failure: effects of ureter ligation and intramuscular injection of glycerol.

The protein binding of diazepam (D) and its major active metabolite N-desmethyl diazepam (DD) was investigated in vitro in the serum of 14 mothers at birth, 21 foetuses at birth, in 100 neonates between...Full Text Available

1984-01-01

466

1 The decreased binding of drugs and dyes to plasma proteins from male and female rats with acute renal failure has been investigated using equilibrium dialysis at 37 degrees C. 2 Acute renal failure...Full Text Available

1979-06-01

467

DNA repair genes

DIMINISHED DEGRADATION OF MYELIN BASIC PROTEIN BY ANTI-SULFATIDE ANTIBODY AND INTERFERON-? IN MYELIN FROM GLIA MATURATION FACTOR-DEFICIENT MICE

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

468

Crystal structures of the N-terminal kinase domain of human RSK1 bound to three different ligands: Implications for the design of RSK1 specific inhibitors

In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin...Full Text Available

2007-06-01

469

Characterization of the major phosphoprotein and its kinase on the surface of the rat adipocyte

The p90 ribosomal S6 kinases (RSKs) also known as MAPKAP-Ks are serine/threonine protein kinases that are activated by ERK or PDK1 and act as downstream effectors of mitogen-activated protein kinase...Full Text Available

2007-12-01

470

Binding the Mammalian High Mobility Group Protein AT-hook 2 to AT-Rich Deoxyoligonucleotides: Enthalpy-Entropy Compensation

Intact rat fat cell exposed to 12.5 ..mu..M (..gamma..-32P)ATP incorporate label into specific proteins within minutes. By solubilizing the reaction mixture with SDS which bypasses the subcellular fractionation steps, the labeled proteins can be identified in autoradiographs of SDS-PAGE gels. The most prominently labeled protein has an M/sub r/ of 42,000. Localization of this component to the cell surface can be made on the basis of inhibition of phosphorylation by addition of a protein derived from the rat brain with protein kinase inhibitory property, susceptibility of the phosphorylated protein to the tryptic digestion, inhibition of phosphorylation of this protein after brief exposure to melittin. To rule out the possibility that the cell surface protein might be a mitochondrial contaminant from broken cells, /sup ...

1986-12-01

471

Basis for the Specificity and Activation of the Serpin Protein Z-dependent Proteinase Inhibitor (ZPI) as an Inhibitor of Membrane-associated Factor Xa*

HMGA2 is a DNA minor-groove binding protein. We previously demonstrated that HMGA2 binds to AT-rich DNA with very high binding affinity where the binding of HMGA2 to poly(dA-dT)₂ is enthalpy-driven...Full Text Available

2009-05-20

472

Association between glucokinase regulatory protein (GCKR) and apolipoprotein A5 (APOA5) gene polymorphisms and triacylglycerol concentrations in fasting, postprandial, and fenofibrate-treated states¹²³

The serpin ZPI is a protein Z (PZ)-dependent specific inhibitor of membrane-associated factor Xa (fXa) despite having an unfavorable P1 Tyr. PZ accelerates the inhibition reaction ∼2000-fold...Full Text Available

2010-06-25

473

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

Background: Hypertriglyceridemia is a risk factor for cardiovascular disease. Variation in the apolipoprotein A5 (APOA5) and glucokinase regulatory protein (GCKR)...Full Text Available

2009-01-01

474

Amino acid sequences that determine the nuclear localization of yeast histone 2B.

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two...Full Text Available

1991-11-01

475

Affinity labelling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-Phe-tRNA

Histone-beta-galactosidase protein fusions were used to identify the domain of yeast histone 2B, which targets this protein to the nucleus. Amino acids 28 to 33 in H2B were required for nuclear localization...Full Text Available

1987-11-01

476

A region in the cytosolic domain of the epidermal growth factor receptor antithetically regulates the stimulatory and inhibitory guanine nucleotide-binding regulatory proteins of adenylyl cyclase.

Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-nitro-4-azidobenzoyl-Phe-tRNA in affinity labelling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates. (author).

477

5'-Azido-[3,6-3H2]-1-napthylphthalamic acid, a photoactivatable probe for naphthylphthalamic acid receptor proteins from higher plants: identification of a 23-kDa protein from maize coleoptile plasma membranes.

Epidermal growth factor (EGF) stimulates adenylyl cyclase in the heart via activation of the stimulatory GTP-binding protein Gs. Therefore, employing peptides corresponding to regions in the cytosolic...Full Text Available

1995-03-14

478

Unusual properties of crocodilian ovomacroglobulin shown in its methylamine treatment and sulfhydryl titration

1-Naphthylphthalamic acid (NPA) is a specific inhibitor of polar auxin transport that blocks carrier-mediated auxin efflux from plant cells. To allow identification of the NPA receptor thought to be...Full Text Available

1992-01-15

479

Trans-activation of the JC virus late promoter by the tat protein of type 1 human immunodeficiency virus in glial cells

The inhibitory activity of chicken and crocodilian ovomacroglobulins against trypsin was measured before and after their incubation with methylamine. The result for crocodilian ovomacroglobulin showed that methylamine treatment destroyed half of its activity, in unique contrast to human alpha 2-macroglobulin and chicken ovomacroglobulin for which methylamine either destroys the inhibitory activity of the former completely or does not affect that of the latter at all. Free sulfhydryl groups of chicken and crocodilian ovomacroglobulins were titrated with 5,5'-dithiobis(2-nitrobenzoic acid) before and after incubation with trypsin. Prior to the incubation with trypsin the chicken and crocodilian proteins respectively had 0 and 1 titratable sulfhydryl per molecule of Mr 720,000. After treatment with trypsin the crocodilian protein had 3.5-4 titratable sulfhydryls, whereas there were no titratable sulfhydryls in the chicken ...

1986-02-01

480

Studies on metabolism of directly labeled {sup 99}MTc-antibody in mice

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC virus (JCV), a human papovavirus. PML is a relatively rare disease seen predominantly in immunocompromised individuals and is a frequent complication observed in AIDS patients. The significantly higher incidence of PML in AIDS patients than in other immunosuppressive disorders has suggested that the presence of human immunodeficiency virus type 1 (HIV-1) in the brain may directly or indirectly contribute to the pathogenesis of this disease. In the present study the authors have examined the expression of the JCV genome in both glial and non-glial cells in the presence of HIV-1 regulatory proteins. They find that the HIV-1-encoded trans-regulatory protein tat increases the basal activity of the JCV late promoter, JCV{sub L}, in glial cells. They conclude that the presence of the HIV-1-encoded tat protein ...

1990-05-01

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481

Proteomic analysis of the shistosome tegument and its surface membranes

The elucidation on the metabolic products of the {sup 99}mTc-antibody conjugates may provide insights and approaches that would reduce the undesirable deposition of radioactive species in normal tissue. In this investigation, the radiolabeled species in blood, urine, bile and extracts of liver and kidney obtained at different times after the injection of a model antibody, {sup 99}mTc, into mice were analyzed with various chromatographic methods. Ninety-nine to 100% of the radioactivity in serum was associated with intact Mab 170. The radioactivity in liver homogenate extract was strictly protein-bound to either intact Mab or low molecular weight species (LMW). In kidney extracts, the majority of the radioactivity was protein bound {sup 99}mTc, with less than 8% of the activity being non- protein bound . Multiple {sup 99}mTc -containing protein and non-protein species were found in ...

1996-12-01

482

Scientific Electronic Library Online (English)

Abstract in english The tegument surface of the adult schistosome, bounded by a normal plasma membrane overlain by a secreted membranocalyx, holds the key to understanding how schistosomes evade host immune responses. Recent advances in mass spectrometry (MS), and the sequencing of the Schistosoma mansoni transcriptome/genome, have facilitated schistosome proteomics. We detached the tegument from the worm body and enriched its surface membranes by differential extraction, before subjecting t (more) he preparation to liquid chromatography-based proteomics to identify its constituents. The most exposed proteins on live worms were labelled with impearmeant biotinylation reagents, and we also developed methods to isolate the membranocalyx for analysis. We identified transporters for sugars, amino acids, inorganic ions and water, which confirm the importance of the tegument plasma membrane in nutrient acquisition and solute balance. Enzymes, including phosphohydrolases, ...

2006-10-01

483

Proteins of the kidney microvillar membrane

Photochemical reactivity of the homologous proteins. cap alpha. -lactalbumin and lysozyme

Two methods were used to label pig kidney microvillar membrane proteins from the luminal and cytoplasmic surfaces of closed membrane vesicles. The first method was lactoperoxidase-catalysed radioiodination. Lactoperoxidase and glucose oxidase were positioned inside or outside the vesicles, iodination being initiated by adding glucose and "1"2"5I. After electrophoresis of the proteins, asymmetric labelling patterns on radioautographs were observed. However the major disadvantage of this method was the high degree of intramembrane labelling of the fatty acid chains of membrane lipids. The second method overcame this disadvantage. A new hydophilic photoreagent, 3,5-di("1"2"5I)iodo-4-azidobenzenesulphonate, was transported by a Na"+-dependent system into microvillar vesicles, thus permitting labelling from either side of the membrane when the vesicles were photolysed. The activity of several microvillar peptidases survived the labelling reaction ...

484

HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration: Its role in cell survival

The fluorescent behaviour and the photodynamic effect was studied in native and structurally modified lysozyme and ..cap alpha..-lactalbumin. The Tyr residues in lysozyme and ..cap alpha..-lactalbumin show different sensitivities to the photodynamic effect. The effect is zero in the case of Tyr from native lysozyme. In contrast, the Tyr residues in ..cap alpha..-lactalbumin are susceptible to photooxidation, which indicates a greater degree of exposure to the solvent. The three His residues of ..cap alpha..-lactalbumin have different degrees of exposure and show two different kinetics of photooxidation whereas the His residue of lysozyme is photooxidized with a single kinetic. Two photooxidation kinetics were obtained for the Trp residues of both native proteins, an indication that in both cases there are Trp residues that are differently exposed to the solvent. The wavelengths of maximum fluorescent emission of the Trp residues were different for the two ...

1985-04-01

485

British Library Electronic Table of Contents (United Kingdom)

Fish retinal ganglion cells (RGCs) can survive and regrow their axons after optic nerve injury. Injured RGCs express anti-apoptotic proteins, such as Bcl-2, after nerve injury; however, upstream effectors of this anti-apoptotic protein are not yet fully understood. Heat shock proteins (HSPs) play a crucial role in cell survival against various stress conditions. In this study, we focused on HSP70 expression in the zebrafish retina after optic nerve injury. HSP70 mRNA and protein levels increased rapidly 2.3-fold in RGCs by 1-6 h after injury and returned to control levels by 1-3 days. HSP70 transcription is regulated by heat shock factor 1 (HSF1). HSF1 mRNA and phosphorylated-HSF1 protein rapidly increased by 2.2-fold in RGCs 0.5-6 h after injury. Intraocular injection of HSP inhibitor I s...

2011-01-01

486

Expression of ribosomal genes in pea cotyledons at the initial stages of germination

Effects of intersegmental transfers on target location by proteins

The time of appearance of newly synthesized rRNAs and ribosomal proteins (r-proteins) in the ribosomes of pea cotyledons (Pisum sativum L.) during germination was investigated. The ribosomal fraction was isolated and analyzed according to the method of germination of the embryo in the presence of labeled precursors or after pulse labeling of the embryos at different stages of germination. For the identification of newly synthesized rRNAs in the ribosomes we estimated the relative stability of labeled RNAs to the action of RNase, the sedimentation rate, the ability to be methylated in vivo in the presence of (/sup 14/C)CH/sub 3/-methionine, and the localization in the subunits of dissociated ribosomes. The presence of newly synthesized r-proteins in the ribosomes was judged on the basis of the electrophoretic similarity in SDS-disc electrophoresis of labeled polypeptides of purified ribosome preparations and of genuine ...

1986-01-20

487

Aggregation of silica nanoparticles directed by adsorption of lysozyme.

We study a model for a protein searching for a target, using facilitated diffusion, on a DNA molecule confined in a finite volume. The model includes three distinct pathways for facilitated diffusion: (a) sliding - in which the protein diffuses along the contour of the DNA (b) jumping - where the protein travels between two sites along the DNA by three-dimensional diffusion, and finally (c) intersegmental transfer - which allows the protein to move from one site to another by transiently binding both at the same time. The typical search time is calculated using scaling arguments which are verified numerically. Our results suggest that the inclusion of intersegmental transfer (i) decreases the search time considerably (ii) makes the search time much more robust to variations in the parameters of the model and (iii) that the optimal search time occurs in a regime very different than that found for models ...

2008-01-01

488

Development of detection methods for irradiated foods; development of immunological identification of irradiated foods

The interaction of the globular protein lysozyme with silica nanoparticles of diameter 20 nm was studied in a pH range between the isoelectric points (IEPs) of silica and the protein (pH 3-11). The adsorption affinity and capacity of lysozyme on the silica particles is increasing progressively with pH, and the adsorbed protein induces bridging aggregation of the silica particles. Structural properties of the aggregates were studied as a function of pH at a fixed protein-to-silica concentration ratio which corresponds to a surface concentration of protein well below a complete monolayer in the complete-binding regime at pH > 6. Sedimentation studies indicate the presence of compact aggregates at pH 4-6 and a loose flocculated network at pH 7-9, followed by a sharp decrease of aggregate size near the IEP of lysozyme. The structure of the bridged silica aggregates was studied by ...

2011-07-20

489

Science at the Theater: Hot Technology, Cool Science

Enzyme-linked Immunosorbent assay systems for the identification of irradiated egg, pork and chicken was developed. Eggs were irradiated in their shells to 0.5{approx}7kGy. Pork was irradiated to 0.5{approx}3kGy and chicken irradiated to 0.5kGy{approx}5kGy. The most sensitive proteins to irradiation were screened by SDS-PAGE and purified. Ovalbumin from egg, salt soluble protein(p) from pork, and salt soluble protein(c) from chicken showed the most sensitivity to irradiation. To investigate for a practical use in identifying of irradiated egg, pork and chicken, competitive ELISA was performed. The binding activity of ovalbumin to anti-ovalbumin IgG was reduced in a dose-dependent manner by irradiating up to 7kGy, and considerably lowered after irradiating at 7kGy. The concentration of 50% inhibition of ovalbumin to IgG was increased to 1.5(0.5kGy){approx}3.7(7kGy) times in an dose-dependent relationship. The binding ...

2002-04-01

490

ScienceCinema

...and welcome to ...lab also known as berkeley lab my name is jeff miller and ...and a public affairs i'd like to ? ...but space is science center and ...berkeley albany high school science department and berkeley high school science department and oakland high school science ...be a q. and ...here please use and because we wanna make sure that your questions are here ...heard of also for the latest developments on science and technology ...guy we're going to be any more and more new features i hope ...and change ...thank you ? much and thank you for coming on the welcome to my world of and mayotte science journalist and what i've done for the last almost thirty years ...people about things about which there passionate and national religion tonight and ...people to explain the science and and i asking the question so what ...and ...worldwide and what it does best ? uh ...bench science and turn it into reality what do you scientists and ...uh they take scientific theory and they turn ...

491

Insulin regulation of protein biosynthesis in differentiated 3T3 adipocytes. Regulation of glyceraldehyde-3-phosphate dehydrogenase

In vivo footprinting of the human [alpha]-globin locus upstream regulatory element by guanine and adenine ligation-mediated polymerase chain reaction

The effect of insulin on protein biosynthesis was examined in differentiated 3T3-L1 and 3T3-F442A adipocytes. Insulin altered the relative rate of synthesis of specific proteins independent of its ability to hasten conversion of the fibroblast (preadipocyte) phenotype to the adipocyte phenotype. Although more than one pattern of response to insulin was observed, the authors focused on the induction of a Mr 33,000 protein which was identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Exposure of 3T3 adipocytes to insulin throughout differentiation specifically increased GAPDH activity and protein content by 2- to 3-fold as compared to 3T3 adipocytes differentiated in the absence of insulin. These changes in enzyme activity and content could be accounted for by a 4-fold increase in the relative rate of synthesis of GAPDH and a 9-fold increase in hybridizable mRNA levels. ...

1985-10-05

492

Characterization of hyaluronate binding proteins isolated from 3T3 and murine sarcoma virus transformed 3T3 cells

A major regulatory element required for expression of the human [alpha]-globin genes is located 40 kb upstream of the embryonic [zeta]-globin gene. To understand how this and other locus control region (LCR) elements contribute to high-level expression in erythroid cells, we have performed high-resolution, in vivo dimethyl sulfate footprinting. In addition, we have modified the dimethyl sulfate-based ligation-mediated polymerase chain reaction in vivo footprinting procedure to permit the assessment of interactions at guanine and adenine residues, rather than guanines alone. In vivo footprinting of the human [alpha]-LCR element carried on chromosome 16 in a mouse erythroleukemia cell environment revealed protein occupancy at GATA-1, AP-1/NF-E2, and CACC/GGTGG motifs, specific differences compared with in vitro protein binding, and distinct changes in one region upon dimethyl sulfoxide-induced cellular maturation. No protein ...

1992-05-01

493

The N-terminal domain of a-dystroglycan, released as a 38kDa protein, is increased in cerebrospinal fluid in patients with Lyme neuroborreliosis

A hyaluronic acid binding fraction was purified from the supernatant media of both 3T3 and murine sarcoma virus (MSV) transformed 3T3 cultures by hyaluronate and immunoaffinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis resolved the hyaluronate affinity-purified fraction into three major protein bands of estimated molecular weight (M/sub r,e/) 70K, 66K, and 56K which contained hyaluronate binding activity and which were termed hyaluronate binding proteins (HABP). Hyaluronate affinity chromatography combined with immunoaffinity chromatography, using antibody directed against the larger HABP, allowed a 20-fold purification of HABP. Fractions isolated from 3T3 supernatant medium also contained additional binding molecules in the molecular weight range of 20K. This material was present in vanishingly small amounts and was not detected with a silver stain or with (/sup 35/S)methionine label. The three ...

1987-06-02

494

British Library Electronic Table of Contents (United Kingdom)

a-Dystroglycan is an extracellular adhesion protein that is known to interact with different ligands. The interaction is thought to stabilize the integrity of the plasma membrane. The N-terminal part of a-dystroglycan may be proteolytically processed to generate a small 38kDa protein (a-DG-N). The physiological significance of a-DG-N is unclear but has been suggested to be involved in nerve regeneration and myelination and to function as a potential biomarker for neurodegenerative and neuromuscular diseases. In this report we show that a-DG-N is released into different body fluids, such as lachrimal fluid, cerebrospinal fluid (CSF), urine and plasma. To investigate the significance of a-DG-N in CSF we examined the levels of a-DG-N and known neurodegenerative markers in CSF from patients di...

2011-01-01

495

Phorbol esters, protein kinase C, and thyroxine 5 prime -deiodinase in brown adipocytes