UK PubMed Central (United Kingdom)

DYF-13, originally identified in C. elegans within a collection of dye-filling chemosensory mutants, is one of several proteins that have been classified as putatively involved...Full Text Available

International Nuclear Information System (INIS)

This investigation was carried out during the two seasons of 1998/1999 and 1999/2000 at the experimental farm belonging to the plant research department, nuclear research center, atomic energy authority in Inshas to evaluate four lupin mutant lines (L_1, L_2, L_3 and L_4) along with their original mother varieties giza 1 and giza 2 under different levels and nitrogen fertilizer levels. The results indicated that seed yield/ plant and its effective components number and weight of pods/plant were significantly higher in all the mutant lines as compared to their parents either in the first or second seasons. Concerning the effect of irrigation intervals on yield and its components for developed mutants and their mother varieties as well as the results gave an evidence that the wider interval showed the lesser values for yield and most of yield components. Obtained data also revealed that yield attributes ...

Energy Technology Data Exchange (ETDEWEB)

In vitro mutagenesis of functional DNA gene fragments by covalently reactive agents permits one in principle to examine the consequent alterations in DNA sequence directly. I have carried out selective mutagenesis of the tetracycline resistance gene in the plasmid pBR322 using the long wavelength UV light activated reaction of 4,5',8-trimethylpsoralen (TMP). The mutagenized DNA sequence was the EcoR1-Hind III restriction fragment in the vicinity of the Tcsup(R) promoter. Two classes of mutants were obtained. One exhibited a high level of Tc resistance (40-60 ..mu..g/ml) but still lower than the wild-type. Interestingly, these showed no sequence alterations at all in the vicinity of the TMP-reacted fragment. The other class of mutants exhibited low levels of drug resistance (< 20 ..mu..g/ml) and two of those that were sequenced were found to contain a 15-base pair insertion to the right of the original Hind III ...

Science.gov (United States)

Reactions on the ionizing radiation of 14 Cladosporium cladosporioides strains were studied. Only 5 of them displayed radiotropizm. The ability of C. cladosporioides strains 4 and 5 with positive radiotropizm and museum C. cladosporioides strain 396 and its alb-mutant SM without positive radiotropizm to destruct radioactive particles of Chernobyl and explosion origin was studied. Two ways of radioactive particles destruction by C. cladosporioides were established, one of them is a direct way by fungal overgrowth of hot particles and the second one an indirect way only by fungal metabolites. Mycelium of the studied C. cladosporioides strains sorbed radionuclides from radioactive particles during cultivation on the liquid and agarized media. No certain inclinations of the individual strains to accumulation of radionuclides 137Cs or 152Eu were ascertained. PMID:12664550

UK PubMed Central (United Kingdom)

Forty-eight mutants unable to reduce nitrate were isolated from "cowpea" Rhizobium sp. strain 32Hl and examined for nitrogenase activity in culture. All but two of the mutants had nitrogenase activity...Full Text Available

UK PubMed Central (United Kingdom)

The regulation of the glutamate dehydrogenases was investigated in wild-type Neurospora crassa and two classes of mutants altered in the assimilation of inorganic nitrogen, as either...Full Text Available

UK PubMed Central (United Kingdom)

Four new mutants are described whose phenotypic expression affects the length of the head of bacteriophage T4D. All mutants produce some phenotypically normal phage particles. Mutant pt21-34...Full Text Available

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This report concerns investigations in the title conducted by 8 groups of National Institute of Radiological Sciences (NIRS) during the period of 1998-2000. The groups are for investigation of: Effects of p53 tumor suppressor gene in radiation-induced leukemia, Role of atm-gene in dose rate effect of ionizing radiation, Function of DNA-dependent protein kinase catalytic subunit (DNA-PK{sub cs}), Functional complementation of radiation-sensitive mutant M10 cell line by human XRCC4 cDNA expression, Role of radiation-induced apoptosis in digital defects in embryonic mice, Functional analysis of S-phase specific novel nuclear protein NP95 by gene targeting, Role of chemokine in T cell development and lymphomagenesis, and establishment of production techniques of gene-modified mice using embryonic stem cells for genetic analysis of radiation-sensitive genes. The groups describe summaries of their studies and published original articles are also ...

UK PubMed Central (United Kingdom)

The objectives of this study were to select and initially characterize mutants of soybean (Glycine max L. Merr. cv Williams) with decreased ability to reduce nitrate. Selection involved...Full Text Available

UK PubMed Central (United Kingdom)

A barley (Hordeum vulgare L.) mutant (Az34) has been identified with low basal levels of abscisic acid (ABA) and with reduced capacity for producing ABA in response to water stress....Full Text Available

UK PubMed Central (United Kingdom)

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not...Full Text Available

UK PubMed Central (United Kingdom)

All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor...Full Text Available

UK PubMed Central (United Kingdom)

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

UK PubMed Central (United Kingdom)

During formation of the optic projection in astray/robo2 mutant zebrafish, optic axons exhibit rostro-caudal pathfinding errors, ectopic midline crossing and...Full Text Available

International Nuclear Information System (INIS)

(1972). Germany Sharma, RL Himachal Pradesh Univ., Palampur (India).

International Nuclear Information System (INIS)

Sesame varieties/genotypes showed a good response to radiation with gamma rays and treatments with EMS. In M_1 both gamma rays and EMS influenced germination, seedling height, survival of plants and pollen fertility/sterility, producing deleterious effects on these characters. Compared to black seeded genotypes, white seeded ones are more susceptible to radiation. Both gamma rays and EMS produced various types of morphological variations in M_2 generation. These were dwarf plant mutants, mottled and fleshy leaved mutants (sterile), fasciated stem, flower colour, altered phyllotaxy, early, uniculm, capsule size variations, multiple capsule/leaf axil, seed coat colour variations, indehiscent and semi-indehiscent type mutants. Moreover, studies on quantitative characters including seed yield revealed the induction of mutants in both positive and negative directions for such traits which made a good scope ...

UK PubMed Central (United Kingdom)

The chemiosmotic model of energy transduction offers a satisfying and widely confirmed understanding of the action of uncouplers on such processes as oxidative phosphorylation; the uncoupler, by facilitating...Full Text Available

International Nuclear Information System (INIS)

The authors have shown that toxicity of paraquat for Escherichia coli is increased over 1-fold in strains defective in the biosynthesis of spermidine compared to isogenic strains containing spermidine. The increased sensitivity of these spermidine-deficient mutants to paraquat is eliminated by growth in medium containing spermidine or by endogenous supplementation of spermidine by the use of a speE"+D"+ plasmid. No paraquat toxicity is seen in the absence of oxygen, even in amine-deficient strains, indicating that superoxide is the agent responsible for the increased toxicity. However, the specific mechanisms responsible for the increased paraquat toxicity in the spermidine-deficient mutants remain to be determined. The marked sensitivity to paraquat of E. coli deficient in spermidine is of particular interest, since such mutants have no other phenotypic properties that can be easily assayed. This increased sensitivity has ...

International Nuclear Information System (INIS)

Two strains of Rhizobium: Rhizobium Tropici and Mesorhizobium Ciceri nodulating respectively common bean and chickpea were treated by gamma irradiation (60Co) source. Radiosensibility analysis showed that 800 Gy was the biggest dose supported by these two strains. We isolated gamma irradiated resistant strain in order to select mutant of them which can supported salt stress and phosphorus deficiency. Salinity analysis showed that Mesorhizobium Ciceri 835 strain, can tolerate up to 18g/l (273 mM NaCl) of salt, whereas, their irradiation mutants tolerate salinity up to 33g/l (564mM. NaCl) Rhizobium Tropici CIAT899 can survive at 20g/l (342 mM) either for control strain or mutants. Analysis of phosphorus deficiency showed that either Rhizobium Tropici CIAT899, or Mesorhizobium Ciceri 835 can survive in medium without phosphore. Our results permit us to screen mutants tolerant to these stresses wide spread ...

UK PubMed Central (United Kingdom)

Glycine is a major inhibitory neurotransmitter in the spinal cord and brainstem. Recently, in vivo analysis of glycinergic synaptic transmission has been pursued in zebrafish using...Full Text Available

UK PubMed Central (United Kingdom)

A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a level...Full Text Available

UK PubMed Central (United Kingdom)

Two nitrate reductase deficient mutants of soybean (Glycine max [L.] Merr. cv Bragg) were isolated from approximately 10,000 M₂ seedlings, using a direct enzymic assay in...Full Text Available

UK PubMed Central (United Kingdom)

The nr₁ soybean (Glycine max [L.] Merr.) mutant does not contain the two constitutive nitrate reductases, one of which is responsible for enzymic conversion of nitrite to...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The reaction of the retinylidene Schiff base in bacteriorhodopsin (bR) to the water-soluble reagent hydroxylamine is enhanced by greater than 2 orders of magnitude under illumination. We have used this reaction as a probe for changes in Schiff base reactivity during the photocycle of wild-type bR and mutants defective in proton transport. We report here that under illumination at pH 6, the D85N mutant has a 20-fold lower rate and the D212N mutant has a greater than 4-fold higher rate for the light-dependent reaction with hydroxylamine compared with wild-type bR. In contrast, the reactivities of wild-type bR and the D96N and T46V mutants are similar. It has been previously shown that the D96N and T46V replacements have no significant effect on the kinetics of M formation but have dramatic effects on rate of the decay of M. We therefore conclude that the hydroxylamine reaction occurs before formation of ...

International Nuclear Information System (INIS)

An induced mutant, RT-10 (M6 generation) was re-irradiated with 300 Gy of gamma rays. In M2 generation a mutant plant was identified, which characterized with large pods, prominent longitudinal ribbon reticulation and slight constriction. The selected mutant, RT-1010 has the highest pod yield (.3.59 t/fed.vs.2.91 and 2.61 t/fed respectively) and harvest index ( 0.52 vs. 0.42 and 0.45 resp.), the best in fancy pods percentage (57.67 % vs. 38.33 % and 44.17 % resp.), TSMK grade (98.96 % vs. 83.03 % and 84.05 % resp.), the largest pod mass (253.37 g vs . 211.32 g and 222.19 g resp.) and the highest expected oil yield (1370 Kg/fed vs. 895.44 Kg and 868.17 Kg/fed. resp.). With regard to fatty acid compositions, both the selected mutant, RT-1010 and the parental mutant RT-10 have almost a similar high oleic fatty acid (C18:1) percentage (56.35 % and 55.103 % respectively), low linoleic ...

Science.gov (United States)

Basic cross site scripting attacks

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Fourier transform infrared (FTIR) difference spectra have been obtained for the bR----K, bR----L, and bR----M photoreactions in bacteriorhodopsin mutants in which Asp residues 85, 96, 115, and 212 have been replaced by Asn and by Glu. Difference peaks that had previously been attributed to Asp COOH groups on the basis of isotopic labeling were absent or shifted in these mutants. In general, each COOH peak was affected strongly by mutation at only one of the four residues. Thus, it was possible to assign each peak tentatively to a particular Asp. From these assignments, a model for the proton-pumping mechanism of bR is derived, which features proton transfers among Asp-85, -96, and -212, the chromophore Schiff base, and other ionizable groups within the protein. The model can explain the observed COOH peaks in the FTIR difference spectra of bR photointermediates and could also account for other recent results on site-directed ...

UK PubMed Central (United Kingdom)

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

UK PubMed Central (United Kingdom)

We report the use of cysteine-substituted mutants in conjunction with in situ oxidation to determine the physical proximity of a pair of engineered cysteines in the pore region of the voltage-gated...Full Text Available

UK PubMed Central (United Kingdom)

Microglia provide immune surveillance for the brain through both the removal of cellular debris and protection against infection by microorganisms and “foreign” molecules. Upon...Full Text Available

UK PubMed Central (United Kingdom)

We describe the lethal, recessive accelerated-cell-death11 Arabidopsis mutant (acd11). Cell death in acd11 exhibits characteristics of animal apoptosis...Full Text Available

UK PubMed Central (United Kingdom)

A method has been developed to predict the effects of mutations in the p53 cancer suppressor gene. The new method uses novel parameters combined with previously established parameters. The most important...Full Text Available

UK PubMed Central (United Kingdom)

Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial...Full Text Available

UK PubMed Central (United Kingdom)

A novel, quantitative method for detecting poly-3-hydroxybutyrate (PHB) amounts in viable cells was developed to allow for high-throughput screening of mutant libraries. The staining technique was demonstrated...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundA strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased...Full Text Available

UK PubMed Central (United Kingdom)

mRNA is sequestered and turned over in cytoplasmic processing bodies (PBs), which are induced by various cellular stresses. Unexpectedly, in Saccharomyces cerevisiae, mutants of the...Full Text Available

UK PubMed Central (United Kingdom)

Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in travelers to countries where the disease is endemic and causes a major disease burden in the indigenous population,...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundInterest in lycopene metabolism and regulation is growing rapidly because accumulative studies have suggested an important role for lycopene in human health promotion....Full Text Available

UK PubMed Central (United Kingdom)

The neuromodulatory function of dopamine (DA) is an inherent feature of nervous systems of all animals. To learn more about the function of neural DA in Drosophila, we generated mutant...Full Text Available

UK PubMed Central (United Kingdom)

Seven mutants of Escherichia coli were isolated that are sensitive to methyl methane sulfonate but not to UV light. They exhibited decreased host cell reactivation capacity for methyl methane sulfonate-treated...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

A study is made of plant sensitivity to mutagens during the processing of seeds. An examination is made of problems concerned with modification mutability, the nature of plant restoration following irradiation. Particular attention is given to mutation changes. A presentation is made of several features of mutant changes initially selected by separate indicators. An examination is made of the possible study of mutant indicator genetics. General data on the forms and varieties produced by the mutagenesis method are given. The book is designed for breeding specialists and geneticists engaged in the study of mutagenesis. 439 references, 27 figures, 66 table.

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Shewanella oneidensis MR-1 is a model environmental organism that possesses diverse respiratory capacities, including the ability to reduce soluble Cr(VI) to sparingly soluble, less toxic Cr(III). Effective bioremediation of Cr-contaminated sites requires knowledge of the molecular mechanisms and regulation of heavy metal resistance and biotransformation by dissimilatory metal-reducing bacteria. Towards this goal, our ERSP-funded work is focused on the identification and functional analysis of genes/proteins comprising the response pathways for chromate detoxification and/or reduction. Previous transcriptomic profiling and whole-cell proteomic analyses implicated the involvement of a functionally undefined DNA-binding response regulator (SO2426) and a putative azoreductase (SO3585) in the chromate stress response of MR-1. Here we describe a detailed functional analysis of SO2426 and SO3585 in order to begin to understand the role of these proteins in the cellular response to chromate. ...

International Nuclear Information System (INIS)

local peanut cultivars Giza 4 and Giza 5 were exposed to different doses ( 50,100,150,200,250 and 300 Gy)to Co "6"0 gamma ray source . all irradiated materials were cultivated to give the M_1 and M_2 generation , then high yielding mutants were selected from the M_2 generation and cultivated separately to give the M_3 generation. some yield component traits were measured for the three generations (M_1,M_2 and M_3) and M_3 families.variations observed in these traits were attributed to genetic factors. the M_3 selected mutants were artificially infected by aspergillus flavus L spores (aflatoxins-B1 and B2 groups-producer strain). aflatoxin concentrations were estimated and the analysis showed that aflatoxin concentrations in seeds of the M_3 selected mutants from cultivar Giza 4 were higher than those in seeds of the M3 mutants selected from cultivar Giza 5. however, the concentration of aflatoxin ...

International Nuclear Information System (INIS)

Mutations in transcription factors hepatocyte nuclear factors (HNF)-1#alpha# and HNF-1#beta# cause maturity-onset diabetes of the young (MODY) types 3 and 5, respectively. HNF-1#alpha# and HNF-1#beta# mutations are well studied in some tissues, but the mechanism by which HNF-1#alpha# and HNF-1#beta# mutations affect sucrase-isomaltase (SI) transcription in the small intestine is unclear. We studied the effects of 13 HNF-1#alpha# mutants and 2 HNF-1#beta# mutants on human SI gene transcription, which were identified in subjects with MODY3 and MODY5, respectively. Transactivation activity of 11 HNF-1#alpha# and 2 HNF-1#beta# mutants was significantly lower than that of wild (wt)-HNF-1#alpha# and wt-HNF-1#beta#. Furthermore, in co-expression studies with mutant (mu)-HNF-1#alpha#/ wt-HNF-1#beta# and wt-HNF-1#alpha#/mu-HNF-1#beta#, the combination of mu-HNF-1#alpha# (P379fsdelCT and ...

Energy Technology Data Exchange (ETDEWEB)

The authors report that the replacement of Leu-93 in bacteriorhodopsin by Ala (L93A) or Thr (L93T) slows down the photocycle by approximately 100-fold relative to wild-type bacteriorhodopsin. Time-resolved visible absorption spectroscopy and resonance Raman experiments, respectively, show the presence of long-lived O-like and N-like intermediates in the photocycles of the above mutants. We infer the existence of an equilibrium between the N and O intermediates in the photocycles of these mutants. The L93A and L93T mutants exhibit normal proton pumping under continuous illumination, suggesting that the decay of the N and/or O intermediate, and consequently, proton translocation, can be accelerated by the absorption of a second photon. Since the 13-cis----all-trans reisomerization of retinal is completed during the decay of the N and O intermediates, they conclude that the interaction of Leu-93 with retinal is important in ...

Science.gov (United States)

Based on studies on the genetic and molecular basis of Shigella flexneri invasive properties, we have constructed and evaluated a double mutant of S. flexneri serotype 5 for utilization as a live attenuated oral vaccine against shigellosis. The first mutation, icsA, blocks intracellular spread of bacteria as well as cell-to-cell infection. It affects the capacity of the invasive pathogen to form large abscesses in epithelia. The second mutation, iuc, eliminates production of the siderophore aerobactin thus impairing growth of the bacterium within tissues. This double mutant, SC5700 appeared safe when administered intragastrically to macaque monkeys as three doses (5 x 10(10) c.f.u. each) at weekly intervals. Protection against a challenge by the wild type isolate (M90T) was observed 4 weeks after the last vaccine inoculation. Duration of carriage was considerably reduced as compared to the control group in which all animals had developed severe ...

International Nuclear Information System (INIS)

Penicillic acid-negative mutants were obtained from a color mutant derived from Penicillium cyclopium NRRL 1888 through N-methyl-N'-nitro-N-nitrosoguanidine treatment. One mutant (SK2N6) accumulated 6-methyl-1,2,4-benzenetriol, which was not previously known to be a metabolite of P. cyclopium, in addition to orsellinic acid and orcinol. The radioactivity of [1-"1"4C]acetic acid was rapidly incorporated into 6-methyl-1,2,4-benzenetriol in a culture of P. cyclopium SK2N6. Moreover, the radioactivity of ["1"4C]6-methyl-1,2,4-benzenetriol was efficiently incorporated into penicillic acid in a culture of P. cyclopium NRRL 1888. These data indicate that 6-methyl-1,2,4-benzenetriol is a precursor for penicillic acid biosynthesis. The results on the addition of 1,4-dihydroxy-6-methoxy-2-methylbenzene, 6-methoxy-2-methylbenzoquinone (1,4), and 1-O-methylorcinol to a culture of P. cyclopium SK2N6 indicated that only the former two ...

British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

International Nuclear Information System (INIS)

Each of the three cysteine residues in the Escherichia coli RecA protein was replaced with a number of other amino acids. To do this, each cysteine codon was first converted to a chain-terminating amber codon by oligonucleotide-directed mutagenesis. These amber mutants were then either assayed for function in different suppressor strains or reverted by a second round of mutagenesis with oligonucleotides that had random sequences at the amber codon. Thirty-three different amino acid substitutions were obtained. Mutants were tested for three functions of RecA: survival following UV irradiation, homologous recombination, and induction of the SOS response. It was found that although none of the cysteines is essential for activity, mutations at each of these positions can affect one or more of the activities of RecA, depending on the particular amino acid substitution. In addition, the cysteine at position 116 appears to be involved in the ...

ScienceCinema

...energy back into space model global warming the ? is the energy that ...

Science.gov (United States)

The authors describe an effect of suppression of invasion of the guinea pig eye conjunctiva and the HEp-2 epithelial cells by virulent Sh. flexneri bacilli, with a simultaneous administration of the same dose of avirulent shigella mutants, genetically connected with them. The data of morphological study and experiments with 3H-glucose labeled shigellae carried out on the cell species model indicated that the bacterial competition for the specific sites for absorption on the epithelial cells underlay the observed phenomenon. PMID:331774

UK PubMed Central (United Kingdom)

Elite suppressors or controllers (ES) are HIV-1 infected patients who maintain undetectable viral loads without treatment. While HLA-B*57-positive ES are usually infected with virus that is unmutated...Full Text Available

UK PubMed Central (United Kingdom)

An assay method is described for measurement of absolute concentrations of the molybdenum cofactor, based on complementation of the defective nitrate reductase ('apo nitrate reductase') in extracts...Full Text Available

UK PubMed Central (United Kingdom)

In Streptococcus pneumoniae, CpsB, CpsC, and CpsD are essential for encapsulation, and mutants containing deletions of cpsB, cpsC, or cpsD...Full Text Available

International Nuclear Information System (INIS)

Mutagenesis of Lupinus mutabilis was started at the UNA LM (Peru) to obtain mutants with low alkaloid content and early germination. Varieties SCG 25 and Lib 020 were irradiated with gamma radiation. The optimum dose for the SCG 25 variety was 15 Krad and for Lib 020 15 to 20 Krad. The relation between the plant height and radiation dose fits the quadratic polynomial model.

International Nuclear Information System (INIS)

Mutation breeding in one of the cultigens TMV-5 in Sesamum is undertaken with 5 kr, 10 kr, 15 kr, 20 kr, 60 kr and 80 kr, dosages of gamma-rays. 60 kr and 80 kr dosages were found to be lethal from the complete failure of seed germination. The mutagen had a heterotic effect on the different characters and the promising variants obtained in this programme are proposed to be maintained by pureline breeding for their possible release as new varieties. (author).

International Nuclear Information System (INIS)

Tow experiments were carried out in 1981 and 1982 growth seasons at the greenhouse of the department of agriculture for soils and water researches, atomic energy authority, at inshas, to investigate the responses of two mutation derived lines of sesame and the local cultivar giza 25 to the application of micro nutrients. The possible effect of radiation on germination and growth of sesame seed was also studied in a separate experiment conducted in 1985 season, at inshas.

UK PubMed Central (United Kingdom)

α-Galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. The α-galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus...Full Text Available

International Nuclear Information System (INIS)

A single amino acid substitution (Asp #-># Asn) at position 138 of E. coli EF-Tu was induced in the tufA gene by an M13 phage oligonucleotide site-directed mutagenesis protocol. The mutated tufA gene was then subcloned in a plasmid vector and expressed in maxicells. The properties of ["3"5S]methionine labelled mutant and wild type EF-Tu's were compared by in vitro assays. Mutant and wild-type EF-Tu's bound EF-Ts with approximately equal affinities. The 138-Asn mutation greatly reduced the protein's affinity for GDP; however, this mutation dramatically increased the proteins affinity for XDP. The mutant protein forms a stable complex with phe-tRNA and XTP, which binds to ribosomes; whereas, it does not form a complex with phe-tRNA and GTP. These results suggest that in EF-Tu x NDP complexes amino acid residue 138 must interact with the substituent on C-2 of the purine ring. Thus in wild-type EF-Tu Asp-138 would H-bond to ...

CERN Document Server

Sur l'origine des chiffres arabes A. Boucenna 1 From the pagination of an Algerian Arabic manuscript of the beginning of the 19th century,we rediscover the original shape that the Arabic numerals had before passing in Europe and underwent the transformation that gave the modern Arabic numerals. This original shape,whose use disappeared completely, proves that these numerals have their origin in the Arabic letters. Contrary to what some hypotheses pretend, particularly those that present them as drifting of Indian characters, the 10 Arabic numerals that we use are, nothing else, 10 Arabic letters more or less modified and taken in the "Abjadi" order. The hypothesis of the Indian origin of the Arabic numerals is revealed a mistake denied by the shape of the Arabic numerals and by the logic of the right to left representation of the numbers and the algorithm of the elementary ...

CERN Document Server

The problem of a spin 1 charged particle with electromagnetic polarizability, obeying a generalized 15-component quantum mechanical equation, is investigated in presence of the external Coulomb potential. With the use of the Wigner's functions techniques, separation of variables in the spherical tetrad basis is done and the 15-component radial system is given. It is shown that there exists a class of quantum states for which the additional characteristics, polarizability, does not manifest itself anyhow; at this the energy spectrum of the system coincides with the known spectrum of the scalar particle. For j=0 states, a 2-order differential equation is derived, it contains an additional potential term 1/r^{4}. In analogous approach wave functions the generalized particle are examined in presence of external Dirac monopole field. It is shown that there exists one special state with minimal conserved quantum number j_{min}. It this solution, ...

UK PubMed Central (United Kingdom)

BackgroundVariation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin...Full Text Available

UK PubMed Central (United Kingdom)

Ever since the pre-molecular era, the birth of new genes with novel functions has been considered to be a major contributor to adaptive evolutionary innovation. Here, I review the origin and evolution...Full Text Available

UK PubMed Central (United Kingdom)

The evolution of sex remains a hotly debated topic in evolutionary biology. In particular, studying the origins of the molecular mechanisms underlying sexual reproduction and gametogenesis (its fundamental...Full Text Available

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... Originator-supplied keywords include: Turbulators, Heat transfer augmentation, Enhancement, Fire tube boilers. (Author). ...

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... by starting and remaining in that size hotel. Another early study, conducted by Nailon (1968) sampled three general managers ...

Energy Technology Data Exchange (ETDEWEB)

An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface physicochemical properties ...

Science.gov (United States)

We have used a cell-free polymerase I transcription system derived from HeLa cells to study the regulation of human rRNA synthesis. Analysis of deletion mutants spanning the start site of transcription at nucleotide +1 indicates that the control region affecting initiation of human rRNA synthesis is contained within sequences from nucleotides -158 to +18. This promoter region can be subdivided into (i) a central segment of approximately 40 base pair that is required for transcription and (ii) flanking sequences that influence the efficiency of transcription in vitro. We have examined the in vitro transcriptional activity of the human extract under various conditions that are thought to modulate rRNA synthesis in vivo. Cell-free extracts prepared from HeLa cells infected with adenovirus 2 synthesize human rRNA at levels greatly decreased relative to uninfected cell extracts. By contrast, in vitro transcription of human rRNA is stimulated 2- to 3-fold by the addition ...

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The production of cellulolytic enzymes from fungi has been extensively studied. Several mutants of Trichoderma reesei were selected. Most of the studies were carried out on T. reesei, T. viride, T. harzianum, Penicillium funiculosum, Altemaria alternata. Aspergillus phoenicis, A. ustus, A. tamarii, A. japonicus, and A. niger. T. koningii is one of the most active producers of the so-called C, factor, which is indispensable for the rapid and extensive attack on crystal-line cellulose. However, Trichodenna is known to excrete only small amounts of {beta}-glucosidase. Therefore, Trichoderma is supplemented with {beta}-glucosidase from Aspergillus to increase the saccharification rate of cellulose to glucose as the main sugar. Induction of mutations in Trichodenna spp. rather than T. viride as a tool for the enhancement of {beta}-glucosidase activity was reported. Unfortunately, T. reesei is a poor producer of {beta}-glucosidase. On the other hand, T. harzianum M{sub ...

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We report here a study of the mechanisms leading to loss of growth control in chicken embryo fibroblasts transformed by Rous sarcoma virus (RSV). We have been particularly concerned with the role of the src gene in this process, and have used RSV mutants temperature sensitive (ts) for transformation to investigate the nature of the growth regulatory lesion. The two principal findings were (1) the stationary phase of the cell cycle (G{sub 1}) in chick embryo fibroblasts seems to have two distinct regulatory compartments (using the terminology of Brooks et al. we refer to these as 'Q' and 'A' states). When rendered stationary at 41.5 C by serum deprivation, normal cells enter a Q state, but cells infected with the ts-mutant occupy an A state. (2) Whereas normal cells can occupy either state depending on culture conditions, the ts-infected cells, at 41.5 C, do not seem to enter Q even though a known src gene ...

Energy Technology Data Exchange (ETDEWEB)

A tk{sup +/-} mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk{sup +/-} L5178Y cell line, which is used widely to assess the mutagenicity of chemical agents. Treatment of 1G2 cells with the alkylating agent N-ethyl-N-nitrosourea (ENU) resulted in a dose-related increase in tribluorothymidine-resistant colonies. Mutant frequencies of 152 and 296 per 10{sup 6} cells were determined for 0.1 and 0.3 mg/ml doses of ENU, compared with a spontaneous mutant frequency of 15 per 10{sup 6} cells. The data indicate that tk{sup +/-} 1G2 ES cells may be useful for the creation of a transgenic mouse model for assessing in vivo mutation using an endogenous autosomal gene. 45 refs., 2 figs., 1 tab.

Energy Technology Data Exchange (ETDEWEB)

The production and uses are described for extracellular microbial polysaccharides (generically called emulsans) and for a new class of extracellular microbial protein-associated lipopolysaccharides (collectively called alpha-emulsans) produced by Acinetobacter Sp. ATCC 31012, its mutants or recombinants. Also included are the deproteinized lipopolysaccharides (collectively called apoemulsans) obtained from such emulsans, as well as to the divalent metal, ammonium, and quaternary ammonium salts of such emulsans and apoemulsans. The materials can be used (1) in cleaning oil-contaminated vessels used to transport or store crude oil or petroleum fractions in such a manner that the residual oil may be recovered for fuel value or for refining and (2) in enhanced oil recovery.

International Nuclear Information System (INIS)

Plutonium, seized from illicit trafficking in nuclear materials, can originate from several, distinct sources. The primary focus of nuclear-forensics investigations of confiscated Pu is to specify correctly its origin. Such specification is possible by means of accurate determination the Pu isotopic composition. Five Pu samples of different origins were analysed by thermal-ionisation mass spectrometry. The source (i.e., the type of production reactor) of each sample was determined by comparing the measured Pu isotopic composition with that calculated by one of two reactor-production computer codes, ORIGEN2 and SCALE. These results, in conjunction with the age determination of each sample, allow us to deduce the origin of each. (author)

International Nuclear Information System (INIS)

Assessment of the roles of the carboxyl-terminal #beta#146 histidyl residues in the alkaline Bohr effect in human and normal adult hemoglobin by high-resolution proton nuclear magnetic resonance spectroscopy requires assignment of the resonances corresponding to these residues. By a careful spectroscopic study of human normal adult hemoglobin, enzymatically prepared des(His146#beta#)-hemoglobin, and the mutant hemoglobins Cowtown (#beta#146His #-># Leu) and York (#beta#146His #-># Pro), the authors have resolved some of these conflicting results. By a close incremental variation of pH over a wide range in chloride-free 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid buffer, a single resonance has been found to be consistently missing in the proton nuclear magnetic resonance spectra of these hemoglobin variants. The results indicate that the contribution of the #beta#146 histidyl residues is 0.52 H"+/hemoglobin tetramer at pH 7.6, markedly less ...

Energy Technology Data Exchange (ETDEWEB)

The origin of natural gas in the Japanese oil and gas fields are geochemically studied. Samples are taken from structural natural gas, surface gas seepage, water-dissolved natural gas and coal-field gas of the Japan Sea coast area. The origins of primary hydrocarbons are classified into bacteria gas and thermogenic gas, the latter being subclassified into gas in oil production zone with the per million deviation of the carbon isotope (/sup 13/C/sup 1/) of methane less than -35 and those gases with maturity higher than this. Surface gas seepage is subjected to migration and bacterial oxidation. Coal gas is similar to oil gas. The CO/sub 2/ concentration is 0 - 2% and /sup 13/CO/sub 2/ deviations from -30 - +30. The origin can be barely estimated from the isotopic composition because of the wide secondary change in the composition. The origin and migration of gas from the gas field of northern Niigata are ...

DEFF Research Database (Denmark)

In order to identify cells of maternal origin in CVS cultures, tissue from 1st trimester abortions were cultivated and the cultures stained in situ for X-chromatin. Convoluted cells and maternal fibroblasts were found to be positive. By chromosome analysis of cultures from 105 diagnostic placenta biopsies, obtained by the transabdominal route, metaphases of maternal origin were found in nine cases. In eight of these cases colonies of convoluted cells were observed. We conclude that convoluted cells are of maternal origin and are a reliable marker for maternal cell contamination in CVS cultures.

UK PubMed Central (United Kingdom)

The present study describes an efficient chemoenzymatic method for introducing a core N-glycan of glycoprotein origin into various lipophilic natural products. It was found...Full Text Available

Science.gov (United States)

(telescopes, cameras, mirrors, solar cookers); Explain to students that the Genesis spacecraft has a concentrator that collects special types of atoms from ...

UK PubMed Central (United Kingdom)

The potential of radiolabelled phenylpiperazines as agents for the detection and therapy of tumours of neural crest origin was evaluated by in vitro pharmacological studies with human neuroblastoma...Full Text Available

International Nuclear Information System (INIS)

The determination of the origin of seized nuclear material is important for authorities in the context of the criminal investigation, in order to return the material to its last legal owner and to help preventing any further diversion of material from this source. Origin determination is based on a complex pattern of parameters obtained through analytical measurements. The information required to determine the origin of nuclear materials may be divided into two categories: endogenous information (e.g. age or mode of production of the material) which is self-explanatory; whereas exogenous information (e.g. dimensions, surface roughness, impurities) requires a database to which the parameters can be compared. The Institute for Transuranium Elements has developed methods to determine characteristic parameters like impurities, surface roughness, or microstructural information. Furthermore, a database was set up containing ...

Science.gov (United States)

... some Central Asian populations (Zerjal et al. 2002). Haplogroup W has a similar Middle Eastern origin, and again ... ...

Science.gov (United States)

More on this story Full text of original item from Northern Arizona University, Apr 04, 2007. Related news stories. Jul 02, 2007 | SCIENCE ...

UK PubMed Central (United Kingdom)

During instrumentation of the root canal, it is important to develop a continuously tapered form and to maintain the original shape and position of the apical foramen. However, the presence of curvatures...Full Text Available

Science.gov (United States)

We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expression of nine new and one previously reported (CcnA) sRNAs in strain D39. CcnA is one of five redundant sRNAs reported by Halfmann et al. (A. Halfmann, M. Kovacs, R. Hakenbeck, and R. Bruckner, Mol. Microbiol. 66:110-126, 2007) that are positively controlled by the CiaR response regulator. We characterized 3 of these 14 sRNAs: Spd-sr17 (144 nucleotides [nt]; decreased in stationary phase), Spd-sr37 (80 nt; strongly expressed in all growth phases), and CcnA (93 nt; induced by competence stimulatory peptide). Spd-sr17 and CcnA likely fold into structures containing single-stranded regions between hairpin structures, whereas Spd-sr37 forms a base-paired structure. Primer ...

British Library Electronic Table of Contents (United Kingdom)

Bacillus licheniformis ?-glutamyltranspeptidase (BlGGT) undergoes an autocatalytic process to generate 44.9 and 21.7?kDa subunits; however, a mutant protein (T399A) loses completely the processing ability and mainly exists as a precursor. For a comprehensive understanding of their structural features, the biophysical properties of these two proteins were investigated by circular dichroism and fluorescence spectroscopy. Tryptophan fluorescence and circular dichroism spectra were nearly identical for BlGGT and T399A, but unfolding analyses revealed that these two proteins had a different sensitivity towards temperature- and guanidine hydrochloride (GdnHCl)-induced denaturation. BlGGT and the unprocessed T399A displayed T m values of 61.4?C and 68.1?C, respectively, and thermal unfolding of b...

Science.gov (United States)

Swollen basiodiospores of an adenine auxotroph of Phanerochaete chrysosporium were protoplasted with Novozyme 234 and transformed to prototrophy by using a plasmid containing the gene for an adenine biosynthetic enzyme from Schizophyllum commune. Transformation frequencies of 100 transformants per {mu}g of DNA were obtained. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Analysis of conidia and basiodiospores from transformants demonstrated that the transforming character was mitotically and meiotically stable on both selective and nonselective media. Genetic crosses between double mutants transformed for adenine prototrophy and other auxotrophic strains yielded Ade{sup {minus}} progeny, which indicated that integration occurred at a site(s) other than the resident adenine biosynthetic gene.

International Nuclear Information System (INIS)

The ability of the R46 R factor and its derivative pKM101 to modify sensitivity to "6"0Co #gamma# radiation was studied. In Escherichia coli K12 both plasmids enhanced bacterial survival after "6"0Co #gamma# irradiation. This effect was dependent on recA"+ genotype but not on recB"+, recB"+recC"+, and recF"+ genotypes. 5-Fluorouracil eliminated the R46 R factor from the parent and its rec"- mutant strains. These strains lost not only the antibiotic resistance coded for R46 R factor but their radioresistance as well.

British Library Electronic Table of Contents (United Kingdom)

Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the thermal expansion component due to heat dissipation of absorbed energy and the negative volume change due to electrostriction induced by charge separation in each of the photosystems could be readily distinguished. Kinetic analysis revealed that PSI and PSII RCs exhibit strikingly different PA signals where PSI is characterized by a strong electrostriction signal and a weak thermal expansion component while PSII has a small electrostriction component and large thermal expansion....

British Library Electronic Table of Contents (United Kingdom)

We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60degreeC). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT at hi...

Energy Technology Data Exchange (ETDEWEB)

Since it was introduced in the early 1940's, mutation breeding has been tested on many crops as modern plant breeding. Until now, more than seven hundred varieties have been developed by means of induced mutation, and many of them officially released and approved for registration. Hundreds of papers report the results of mutation breeding, and the characteristics of induced mutation in different kinds of crops were discussed for review purposes (Blixt and Gottschalk 1975, Gottschalk and Wolff 1983). Considering the results already obtained, it can be concluded that the kinds of induced mutation and their utilization vary from crop to crop. This paper summarizes and discusses the mutation characteristics and kinds on the induced mutants of leguminous plants that have been released.

British Library Electronic Table of Contents (United Kingdom)

Abstract Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and...

British Library Electronic Table of Contents (United Kingdom)

Maintenance of genomic integrity in embryonic cells is pivotal to proper embryogenesis, organogenesis and to the continuity of species. Cultured mouse embryonic stem cells (mESCs), a model for early embryonic cells, differ from cultured somatic cells in their capacity to remodel chromatin, in their repertoire of DNA repair enzymes, and in the regulation of cell cycle checkpoints. Using 129XC3HF1 mESCs heterozygous for Aprt, we characterized loss of Aprt heterozygosity after exposure to ionizing radiation. We report here that the frequency of loss of heterozygosity mutants in mESCs can be induced several hundred-fold by exposure to 5-10Gy of X-rays. This induction is 50-100-fold higher than the induction reported for mouse adult or embryonic fibroblasts. The primary mechanism underlying the...

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

Energy Technology Data Exchange (ETDEWEB)

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

International Nuclear Information System (INIS)

Cell surface glycosaminoglycans play important roles in cell adhesion and viral entry. Laboratory strains of two alphaviruses, Sindbis and Semliki Forest virus, have been shown to utilize heparan sulfate as an attachment receptor, whereas Ross River virus (RRV) does not significantly interact with it. However, a single amino acid substitution at residue 218 in the RRV E2 glycoprotein adapts the virus to heparan sulfate binding and expands the host range of the virus into chicken embryo fibroblasts. Structures of the RRV mutant, E2 N218R, and its complex with heparin were determined through the use of electron cryo-microscopy and image reconstruction methods. Heparin was found to bind at the distal end of the RRV spikes, in a region of the E2 glycoprotein that has been previously implicated in cell-receptor recognition and antibody binding.

Science.gov (United States)

The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation. PMID:7604032

British Library Electronic Table of Contents (United Kingdom)

Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a sing...

British Library Electronic Table of Contents (United Kingdom)

Mutations or multiplications in a-synuclein gene cause familial forms of Parkinson disease or dementia with Lewy bodies (LB), and the deposition of wild-type a-synuclein as LB occurs as a hallmark lesion of these disorders, collectively referred to as synucleinopathies, implicating a-synuclein in the pathogenesis of synucleinopathy. To identify modifier genes of a-synuclein-induced neurotoxicity, we conducted an RNAi screen in transgenic C. elegans (Tg worms) that overexpress human a-synuclein in a pan-neuronal manner. To enhance the RNAi effect in neurons, we crossed a-synuclein Tg worms with an RNAi-enhanced mutant eri-1 strain. We tested RNAi of 1673 genes related to nervous system or synaptic functions, and identified 10 genes that, upon knockdown, caused severe growth/motor abnormalit...

Science.gov (United States)

An original method is proposed for ensuring time-invariant (stationary) interior ballistic parameters in the postprojectile space of a gun barrel. Stationarity of the parameters is achieved by investing the solid-propellant charge with highly original structures that produce the required pressure condition and linear growth of the projectile velocity. Simple relations are obtained for calculating the principal characteristics.

UK PubMed Central (United Kingdom)

Brushite (CaHPO₄·2H₂O) was considered to govern the formation of renal calculus of calcium phosphate origin. The degree of saturation of urine with respect to this phase...Full Text Available

CERN Document Server

We conjecture that all CP violations (both Dirac and Majorana types) arise from a common origin in neutrino seesaw. With this conceptually attractive and simple conjecture, we deduce that mu-tau breaking shares the common origin with all CP violations. We study the common origin of mu-tau and CP breaking in the Dirac mass matrix of seesaw Lagrangian (with right-handed neutrinos being mu-tau blind), which uniquely leads to inverted mass-ordering of light neutrinos. We then predict different correlations between the two small mu-tau breaking observables theta_{13} - 0 and theta_{23} -45, which can be tested against our previous normal mass-ordering scheme by the on-going oscillation experiments. We also analyze the correlations of theta_{13} with Jarlskog invariant and neutrinoless double-beta-decay observable. From the common origin of CP and mu-tau breaking in the neutrino seesaw, we establish a direct ...

Energy Technology Data Exchange (ETDEWEB)

We have observed significant differences in the response to ionizing radiation of two closely related human cell lines, and now compare the effects on these lines of both low and intermediate LET radiation. Compared to TK6, WTK1 has an enhanced X-ray survival, and is also more resistant to cell killing by {alpha}-particles. The hprt locus is more mutable in WTK1 than in TK6 by both X-rays and {alpha}-particles. WTK1 is also more mutable by {alpha}-particles than by X-rays at the hprt locus. X-ray-induced mutation at the heterozygous tk locus in WTK1 is about 25 fold higher than in TK6, while {alpha}-particle-induced mutation is nearly 50 fold higher at this locus. Also, the slowly growing tk- mutants, which comprise the majority of spontaneous and X-ray-induced tk- mutants of TK6, were not induced significantly by {alpha}-particles. Previously, we showed that TK6 has a reduced capacity for recombination compared with WTK1, and therefore, these ...

Energy Technology Data Exchange (ETDEWEB)

ATR-X (alpha-thalassemia/mental retardation, X-linked) syndrome is a human congenital disorder that causes severe intellectual disabilities. Mutations in the ATRX gene, which encodes an ATP-dependent chromatin-remodeler, are responsible for the syndrome. Approximately 50% of the missense mutations in affected persons are clustered in a cysteine-rich domain termed ADD (ATRX-DNMT3-DNMT3L, ADD{sub ATRX}), whose function has remained elusive. Here we identify ADD{sub ATRX} as a previously unknown histone H3-binding module, whose binding is promoted by lysine 9 trimethylation (H3K9me3) but inhibited by lysine 4 trimethylation (H3K4me3). The cocrystal structure of ADD{sub ATRX} bound to H3{sub 1-15}K9me3 peptide reveals an atypical composite H3K9me3-binding pocket, which is distinct from the conventional trimethyllysine-binding aromatic cage. Notably, H3K9me3-pocket mutants and ATR-X syndrome mutants are defective in both H3K9me3 binding and ...

Energy Technology Data Exchange (ETDEWEB)

Courant-Snyder (CS) theory for one degree of freedom has recently been generalized by Qin and Davidson to the case of coupled transverse dynamics with two degrees of freedom. The generalized theory has four basic components of the original CS theory, i.e., the envelope equation, phase advance, transfer matrix, and the CS invariant, all of which have their counterparts in the original CS theory with remarkably similar expressions and physical meanings. In this brief communication, we further extend this remarkable similarity between the original and generalized CS theories and construct the Twiss parameters and beam matrix in generalized forms for the case of a strong coupling system.

CERN Document Server

To investigate the origin and nature of inertia, we introduce a new concept of hypothetical 2D, so-called, "master-space" (MS), subject to certain rules. The MS, embedded in the background 4D-spacetime, is an indispensable individual companion to the particle of interest, without relation to every other particle. We argue that a deformation/(distortion of local internal properties) of MS is the origin of inertia. With this perspective in sight, we construct the alternative relativistic theory of inertia (RTI), which allows to compute the relativistic inertial force acting on an arbitrary point-like observer due to its "absolute acceleration". We go beyond the hypothesis of locality with an emphasis on distortion of MS, which allows to improve essentially the standard metric and other relevant geometrical structures related to the noninertial reference frame of an arbitrary accelerated observer. We compute the inertial force exerted on the ...

Energy Technology Data Exchange (ETDEWEB)

Forensic signatures are present in any Pu sample that can determine the sample`s origin: isotopic ratio of Pu, progeny species that grow into the sample, and contaminant species left over from incomplete purification of the Pu in fuel reprocessing. In the context of intelligence information, this can result in attribution of responsibility for the product of clandestine proliferant operations or material smuggled from existing stockpiles. A list of signature elements and what can be determined from them have been developed. Work needs to be done in converting concentrations of signature species into a quantitative forensic analysis, particularly in regard to reactor performance, but this should require only a small effort. A radiochemical analysis scheme has been developed for measuring these nuclides; more work is needed, particularly for determining fission product concentrations. A sample of Pu metal has been analyzed and several parameters determined that are ...

UK PubMed Central (United Kingdom)

The number needed to treat (NNT) is a popular effect measure to present study results in biomedical research. NNTs were originally proposed to describe the absolute effect of a new treatment compared...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The original plan for support of research in geothermal reservoir engineering, GREMP, is reviewed and compared with accomplishments to date. A commentary on the comparison and an updated plan are presented. Also included are a justification of the updated plan and a revised management plan. (MHR)

Science.gov (United States)

Original holdings are now kept by the World Data Centre C1 at RAL: http://wdcc1. bnsc.rl.ac.uk MODS DOY = 246 ADID_REF Not applicable LOGICAL_FILE_ID ...

UK PubMed Central (United Kingdom)

Most breast cancers are multicentric in origin. They drain into two primary lymphatic depots—the axilla and internal mammary chain of nodes. The incidence of metastasis to the internal mammary...Full Text Available

UK PubMed Central (United Kingdom)

Part One of this article describes the principles and origins of the Healthy Communities movement. Part Two describes the Coalition for Healthier Cities and Communities, a national network of partnerships...Full Text Available

UK PubMed Central (United Kingdom)

Isokinetic training and assessment of the knee joint has been the mainstay of rehabilitation, especially in patients with anterior cruciate ligament deficiency. Besides the original shin pad used, the...Full Text Available

UK PubMed Central (United Kingdom)

In rats, expression of the immediate early gene, c-fos observed in the brain following male copulatory behavior relates mostly to the detection of olfactory information originating...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundIn β-thalassemia, profound anemia and severe hemosiderosis cause functional and physiological abnormalities in various organ systems. In recent years, there have...Full Text Available

UK PubMed Central (United Kingdom)

The phylogeny and taxonomy of mammalian species were originally based upon shared or derived morphological characteristics. However, genetic analyses have more recently played an increasingly important...Full Text Available

UK PubMed Central (United Kingdom)

The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing...Full Text Available

UK PubMed Central (United Kingdom)

A 6-year-old female, intact boxer presented with acute respiratory distress due to a mass in the left cranial thorax. The tumor, which originated in the left ...Full Text Available

UK PubMed Central (United Kingdom)

Despite the health-related benefits of exercise, many people do not engage in enough activity to realize the rewards, and little is known regarding the genetic or environmental components that account...Full Text Available

UK PubMed Central (United Kingdom)

cdc18+ of Schizosaccharomyces pombe is a periodically expressed gene that is required for entry into S phase and for the coordination of S phase with mitosis. cdc18+ is related to the Saccharomyces...Full Text Available

UK PubMed Central (United Kingdom)

Retroviruses are well known pathogens of mammals, birds and fish. Their potential to induce cancer in chickens was already described almost 100 years ago and murine retroviruses have been a subject...Full Text Available

CERN Document Server

We argue that neutron interference experiments and experiments on gravitational bound states of neutron unambiguously disprove entropic origin of gravitation. The criticism expressed in a recent paper [arXiv:1104.4650] concerning our arguments against entropic gravity is shown to be invalid.

Wastenet

... Buckeye rapidly produces high resolution imagery over a commander's area of interest contributing to intelligence, surveillance and reconnaissance, and also creates detailed mapping of the battlespace. BIG uses an innovative, original and creative combination of Web programming languages, Geographic Information Systems, and ...

UK PubMed Central (United Kingdom)

Vocal learning is a critical behavioral substrate for spoken human language. It is a rare trait found in three distantly related groups of birds-songbirds, hummingbirds, and parrots. These avian groups...Full Text Available

UK PubMed Central (United Kingdom)

Special red cells were found on the adaxial surface of tertiary pulvini of Mimosa pudica and experiments performed to determine the origin and function of these cells. Using anatomical...Full Text Available

UK PubMed Central (United Kingdom)

Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using...Full Text Available

UK PubMed Central (United Kingdom)

Gastric carcinoids (GCs), which originate from gastric enterochromaffin-like (ECL) mucosal cells and account for 2.4% of all carcinoids, are found increasingly in the course of upper gastrointestinal...Full Text Available

UK PubMed Central (United Kingdom)

All eukaryotes require mitochondria for survival and growth. The origin of mitochondria can be traced down to a single endosymbiotic event between two probably prokaryotic organisms. Subsequent evolution...Full Text Available

UK PubMed Central (United Kingdom)

The question of whether proteins originate from random sequences of amino acids is addressed. A statistical analysis is performed in terms of blocked and random walk values formed by binary hydrophobic...Full Text Available

Science.gov (United States)

... M. Taylor, T. Kurt Kyser. (2009) Feather isotope analysis discriminates age-classes of Western, Least, and Semipalmated sandpipers when plumage ... ...

UK PubMed Central (United Kingdom)

Ewing's sarcoma is the second most common bone malignancy affecting children and young adults. The prognosis is especially poor in metastatic or relapsed disease. The cell of origin remains elusive,...Full Text Available

UK PubMed Central (United Kingdom)

Comorbidities of hidradenitis suppurativa (acne inversa) were reviewed by extracting original and review publications included in MEDLINE, EMBASE and COCHRANE libraries using the terms “hidradenitis,”...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe rate of emergence of human pathogens is steadily increasing; most of these novel agents originate in wildlife. Bats, remarkably, are the natural reservoirs of many...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

This document contains a listing of the written scientific information originating in the Materials Joining (formerly the Welding and Brazing Group), Metals and Ceramics Division, Oak Ridge National Laboratory during 1951 through June 1991. This registry of documents is as much as possible, in the order of issue date.

UK PubMed Central (United Kingdom)

Autophagy is an evolutionarily conserved degradative pathway that has been implicated in a number of physiological events important for human health. This process was originally identified as a response...Full Text Available

International Nuclear Information System (INIS)

A brief letter attempts to justify the assumption in an earlier paper of an isotropic distribution of environmental gamma rays incident on humans. A second letter from the original authors responds to this in yet more detail. (UK).

UK PubMed Central (United Kingdom)

Alton Ochsner was a giant of American surgery. His career encompassed patient care, teaching, and research as symbolized on the original seal of the Ochsner Clinic. His ideas were innovative and groundbreaking...Full Text Available

UK PubMed Central (United Kingdom)

The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies...Full Text Available

UK PubMed Central (United Kingdom)

Thiamin(e), also known as vitamin B1, is now known to play a fundamental role in energy metabolism. Its discovery followed from the original early research on the ‘anti-beriberi factor’...Full Text Available

UK PubMed Central (United Kingdom)

In the yeast Saccharomyces cerevisiae, a and α mating-type information is stored in transcriptionally silenced cassettes called HML and HMR....Full Text Available

Energy Technology Data Exchange (ETDEWEB)

A general model for wakefield-generated instabilities in linear accelerators, originally developed for cumulative beam breakup [1], is applied to the resistive wall instability. The general solution for various bunch charge distributions and application to various accelerator configurations are presented.

Science.gov (United States)

...Department of Environmental Control to ASARCO Incorporated. (B) Amended Administrative...Department of Environmental Control to ASARCO Incorporated. (C) Second Amended...Department of Environmental Control to ASARCO Incorporated. (ii) Additional...

Energy Technology Data Exchange (ETDEWEB)

A database has been developed to contain the disposal limits for the E-Area Low Level Waste Facility (ELLWF). This database originates in the form of an EXCEL{copyright} workbook. The pertinent sheets are translated to PDF format using Adobe ACROBAT{copyright}. The PDF version of the database is accessible from the Solid Waste Division web page on SHRINE. In addition to containing the various disposal unit limits, the database also contains hyperlinks to the original references for all limits. It is anticipated that database will be revised each time there is an addition, deletion or revision of any of the ELLWF radionuclide disposal limits.

International Nuclear Information System (INIS)

Original investigations of ablation of minerals, fullerene-like compounds, polymers and complicated biological macromolecules under the action of submillimeter radiation of the free-electron laser (FEL) developed and built at Budker Institute of Nuclear Physics [V.P. Bolotin et al., First experiments on high-power Novosibirsk terahertz free-electron laser, Budker INP, 2005, p. 37 [1

International Nuclear Information System (INIS)

Original pictures installed in the 1st and 2nd generation type central control panels (CCP) and new 'Awarable and Complex' pictures were made on personal computers and evaluated. A total 18 of actual plant operators (M=32.3, SD=10.5 years old) participated in the evaluation. The operators rated the new CRT pictures highly. The response times using the new CRT pictures were shorter than those by the original pictures. Both results suggested that the CRT picture design guidelines based on the operators' plant images were effective for improving their performance. (author)

International Nuclear Information System (INIS)

Full text: The maturation of chicken forebrain is protracted and occurs well after synapse formation providing a good model for studying mechanisms of brain maturation. Using microslices from immature (10 day) and adult chicken forebrain prepared after decapitation, we have examined functional properties of NMDA and AMPA receptors by measuring agonist-induced uptake of "4"5Ca"2"+ . The rate and extent of NMDA induced "4"5Ca"2"+ accumulation decreased during maturation with no change in EC_5_0. The rate and extent of the AMPA induced response also decreased with a 60-fold increase in EC_5_0. However, the total NMDA receptor content did not change as indicated by 3 H-MK801 binding and NR1 immunoreactivity in P2 fractions. Similarly, there was no change in the B_m_a_x of "3H-AMPA, though there was a two-fold increase in K_D, and little or no change in the immunoreactivity in GluR1, 2, 2/3 or 4. These results suggest that it is the regulation of receptors, their subunit composition and/or ...

International Nuclear Information System (INIS)

In experiments designed to detect new mutations affecting hemoglobin, we irradiated the male or female parent in reciprocal crosses of two mouse strains that differ in alleles at the hemoglobin (Hba, Hbb) loci as well as at five other specific loci. Offspring were analyzed for hemoglobin properties (electrophoretic pattern, solubility, crystal pattern), serum albumin differences, erythrocyte lysis, reticulocyte count, and external appearance. Five hemoglobin variants were found among the mutants. In three, the genetic contribution from the irradiated father was not expressed with regard to the #alpha#-chain; one carried a tandem duplication (the first known case in the mouse) involving Hbb; and one probably resulted from double nondisjunction of chromosome 7. The finding that major chromosome aberratios can mimic hemoglobin mutations indicates the need, in similar experiments, to follow F_1 screening with thorough cytogenetic analysis. The variants in which there ...

Science.gov (United States)

Functional activation of {beta}-catenin/Tcf signaling plays an important role in early events in carcinogenesis. We examined the effect of naringenin against {beta}-catenin/Tcf signaling in gastric cancer cells. Reporter gene assay showed that naringenin inhibited {beta}-catenin/Tcf signaling efficiently. In addition, the inhibition of {beta}-catenin/Tcf signaling by naringenin in HEK293 cells transiently transfected with constitutively mutant {beta}-catenin gene, whose product is not phosphorylated by GSK3{beta}, indicates that its inhibitory mechanism was related to {beta}-catenin itself or downstream components. To investigate the precise inhibitory mechanism, we performed immunofluorescence, Western blot, and EMSA. As a result, our data revealed that the {beta}-catenin distribution and the levels of nuclear {beta}-catenin and Tcf-4 proteins were unchanged after naringenin treatment. Moreover, the binding activities of Tcf complexes to consensus DNA were not ...

Science.gov (United States)

In mammals and insects, paracellular blood barriers isolate the nervous system from the rest of the animal. Glia and accessory cells of the nervous system use pumps, channels, cotransporters, and exchangers collectively to maintain the extracellular ion environment and osmotic balance in the nervous system. At present, the molecular mechanisms that regulate this process remain unclear. In humans, loss of extracellular ion and volume regulation in the nervous system poses serious health threats. Drosophila is a model genetic organism with a proven track record for uncovering molecular mechanisms relevant to human health and disease. Here, we review what is known about extracellular ion and volume regulation in larval abdominal nerves, present some new data about the impact of neural activity on the extracellular environment, and relate the findings to mammalian systems. Homologies have been found at the level of morphology, physiology, molecular mechanisms, and ...

British Library Electronic Table of Contents (United Kingdom)

We describe a method for the analysis of multi-site phosphorylation in serine/threonine (Ser/Thr)-rich protein sequences. Site-specific mutagenesis was used to introduce tryptic cleavage sites in the serine glutamine/threonine glutamine cluster domain (SCD) of the human checkpoint protein kinase (Chk2). The mutant proteins were shown to autophosphorylate on residues that are inducibly phosphorylated when mammalian cells are exposed to ionizing radiation (serine 33/35, serine 516, threonine 68 and threonine 432). Five Ser/Thr clusters within the SCD were flanked by arginine or lysine residues to produce tryptic peptides for nanospray liquid chromatography (nanoLC)/linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Phosphorylation sites were assigned usin...

British Library Electronic Table of Contents (United Kingdom)

Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50?g/L in 72?h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol prod...

British Library Electronic Table of Contents (United Kingdom)

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in ...

International Nuclear Information System (INIS)

The photobiology of mouse melanocyte lines with different pigment genotypes was studied by measuring colony-forming ability after irradiation. The cell lines were wild-type black (melan-a) and the mutants brown (melan-b) and albino (melan-c). Four lamps emitting various UV wavelengths were used. These were germicidal (UVC, 200-280 nm), 82.3% output at 254 nm, TL01 (UVB, 280-320 nm), 64.2% at 310-311 nm, FS20, broadband with peak output at 312 nm and Alisun-S (UVA, 320-400 nm), broadband with peak output at 350-354 nm. Appropriate filtration reduced the contaminating UVC to nonlethal levels for the longer waverange lamps. It is clear from these studies that, in pigment cells, monochromatic results cannot predict polychromatic responses and that cell death from solar irradiations is a complex phenomenon that depends on more than DNA damage. (author).

Science.gov (United States)

Group B Streptococcus (GBS) is the leading cause of meningitis in newborn infants. Bacterial cell surface appendages, known as pili, have been recently described in streptococcal pathogens, including GBS. The pilus tip adhesin, PilA, contributes to GBS adherence to blood-brain barrier (BBB) endothelium; however, the host receptor and the contribution of PilA in central nervous system (CNS) disease pathogenesis are unknown. Here we show that PilA binds collagen, which promotes GBS interaction with the ?(2)?(1) integrin resulting in activation of host chemokine expression and neutrophil recruitment during infection. Mice infected with the PilA-deficient mutant exhibit delayed mortality, a decrease in neutrophil infiltration and bacterial CNS dissemination. We find that PilA-mediated virulence is dependent on neutrophil influx as neutrophil depletion results in a decrease in BBB permeability and GBS-BBB penetration. Our results suggest that the bacterial pilus, ...

Energy Technology Data Exchange (ETDEWEB)

Guard cells rely on import for their supply with reduced carbon. The authors tested by silicone oil centrifugation the ability of guard cell protoplasts to accumulated ({sup 14}C)-sucrose. Uptake rates were corrected after measurement of {sup 14}C-sorbitol and {sup 3}H{sub 2}O spaces. Sucrose uptake followed biphasic kinetics, with a high-affinity component below 1 mM external sucrose (apparent K{sub m} 0.8 mM at 25C) and a low-affinity nonsaturable component above. Uptake depended on pH (optimum at pH 5.0). Variations in the concentrations of external KCl, CCCP, and valinomycin indicated that about one-half of the sucrose uptake rate could be related to an electrochemical gradient across the plasmalemma. Total uptake rates measured at 5 mM external sucrose seem to be sufficient to replenish emptied plastids with starch within a few hours.

Energy Technology Data Exchange (ETDEWEB)

The simulation of the Auxiliary Charcoal Bed (ACB) Vacuum System was performed to evaluate the original vacuum system design, detect and identify design deficiencies, investigate the effects of proposed corrections on system performance, and generally aid in refining the system design before construction and mockup testing. The simulation was performed by using the Advanced Continuous Simulation Language (ACSL). The vacuum system design goals are to provide approximately 20 SCFM of both booster gas and purge gas through the system and maintain a flow of approximately 40 SCFM with a velocity of 50 to 75 f/sec at the entrance to the cyclone separator. The model results showed that the original system design was incapable of meeting the system performance goals. Further simulations showed that the following modifications to the original vacuum system design were required to make the system performance acceptable; (1) Remove ...

Energy Technology Data Exchange (ETDEWEB)

This document is the Final Report for the project. Improvements in SISCOM II Technology. It consists of a brief description of the activities carried out during the project, and several Annexes that explain in a deeper way each result. SISCOM II is the main telemetry system currently in use in the Spanish underground coal mining, but it was developed ten years ago and needed a technological update, which was the objective of this project. The aims of the project were: - To develop Hardware and Software for the Outstations currently used (maintenance developments) - To design new hardware and software, easier to use in the current and new applications of SISCOM II Both objectives were met, although not in the way originally intended. The main results were: In the field of old Outstations update: - A new set of boards for the old Outstations was developed - Outstations`s firmware was updated for the new hardware In the field new hardware and software design: - SISCOM ...

CERN Document Server

The origin of life and the origin of the universe represent two of the most important problems of science. Both are resolved by hydro-gravitational dynamics (HGD) cosmology (Gibson 1996, Schild 1996, Gibson 2009ab), which predicts frozen primordial hydrogen-helium gas planets in clumps as the dark matter of galaxies. Merging Earth-mass planets formed stars, moons and comets to incubate and cosmically seed the first life. Cometary panspermia (Hoyle and Wickramasinghe 1981, 1982; Wickramasinghe et al. 2009) occurs naturally by HGD mechanisms. Comets and moons are fragments from mergers of stardust covered frozen gas planets in their step-wise growth to star mass. Supernovae from stellar over-accretion of planets produce stardust (C, N, O, P etc.) chemical fertilizer. Planets collect this infected radioactive dust gravitationally, to provide liquid water domains in contact with life nutrients seeded with life prototypes. The first mutating, ...

Energy Technology Data Exchange (ETDEWEB)

The Savannah River Swamp is a 3,020 Ha forested wetland on the floodplain of the Savannah River and is located on the Department of Energy`s Savannah River Site (SRS). Major impacts to the swamp hydrology occurred with the completion of the production reactors and one coal-fired powerhouse at the SRS in the early 1950`s. Water was pumped from the Savannah River, through secondary heat exchangers of the reactors, and discharged into three of the tributary streams that flow into the swamp. This continued from 1954 to 1988 at various levels. The sustained increases in water volume resulted in overflow of the original stream banks and the creation of additional floodplains. Accompanying this was considerable erosion of the original stream corridor and deposition of a deep silt layer on the newly formed delta. Heated water was discharged directly into Pen Branch and water temperature in the stream often exceeded 50 C. The nearly continuous flood of ...

CERN Document Server

Constraints on the original Cardassian model and the modified polytropic Cardassian model are examined from the latest derived 397 Type Ia supernova (SNe Ia) data, the size of baryonic acoustic oscillation peak from the Sloan Digital Sky Survey (SDSS), the position of first acoustic peak of the Cosmic Microwave Background radiation (CMB) from the five years Wilkinson Microwave Anisotropy Probe (WMAP), the x-ray gas mass fractions in clusters of galaxies, and the observational H(z) data. In the original Cardassian model with these combined data set, we find $\\Omega_{m0}=0.271^{+0.014}_{-0.014}, n=0.035^{+0.049}_{-0.049}$ at $1 \\sigma$ confidence level. And in the modified polytropic Cardassian model, we find that $\\Omega_{m0}=0.271^{+0.014}_{-0.015}$, $n=-0.091^{+0.331}_{-1.908}$ and $\\beta=0.824^{+0.750}_{-0.622}$ within $1\\sigma$ confidence level. According to these observations, the acceleration of the universe begins at ...

Energy Technology Data Exchange (ETDEWEB)

Recent organic geochemical studies differ radically in their interpretation of the origin of the Gulf coast oils. However all studies since (and including) Thompson, et al. 1989, emphasize the considerable geographic variation in genetic oil types and are in generalized agreement as to the genetic oil types present and their spatial and stratigraphic relationships. In particular it is accepted that the Gulf rim (both north and south) is encircled by a ring of Mesozoic carbonate-sourced oils and that there is also at least one major deep water trend of carbonate-sourced oils in the northern Gulf. It is also generally agreed that many of the Gulf rim oils are of Oxfordian-Kimmeridgian age, though some, like the Sunniland oils of Florida, are of lower Cretaceous origin. Major disagreement still occurs over the sources of the Louisiana offshore oils and onshore Wilcox reservoired oils. One {open_quotes}school of thought{close_quotes} assigns all ...

Energy Technology Data Exchange (ETDEWEB)

The computer programme COMMIX-2 describes steady state and transient multidimensional single- and two-phase fluid flows with heat transfer in nuclear reactor components and multicomponent systems. Originally from the Argonne National Laboratory, the code has been further developed at the Kernforschungszentrum Karlsruhe. The original Point-SOR iterative method for the solution of a Poisson-like equation describing the pressure distribution in the fluid as well as the transport of enthalpy and turbulent quantities has been complemented with iterative and direct line- and block-methods. None of the newly implemented methods is original in itself but their implementation into the computer code, which can describe the most general shapes of definition domains, gave a code speed-up by a factor of 2-5, depending on the problem treated. The code capabilities are assessd by the calculation of a benchmark problem involving the ...

International Nuclear Information System (INIS)

The identification, and hence origin determination, of unknown nuclear material that might be found undeclared away from designated locations in the nuclear fuel cycle, is an important task in the frame of nuclear forensics. Material with forensic importance can be found at the microscopic level as particles in environmental samples indicating possible clandestine production of fissile material, and as bulky samples in the case of illicit trafficking of nuclear material. The objective of this work is to present, at a theoretical level, an isotopic finger-printing methodology which would determine the origin of unknown nuclear material with forensic importance. This is demonstrated for the case when the unknown nuclear material is spent nuclear fuel. The methodology is based on multivariate statistics, such as cluster and factor analysis, complemented by spent fuel isotopic composition simulations using the zero-dimensional depletion computer ...

International Nuclear Information System (INIS)

Different types of radioactive liquid waste are being generated at Advanced Fuel Fabrication Facility (AFFF), Tarapur during the quality control analysis of the mixed oxide (MOX) fuel pellets. A laboratory scale study was performed for treatment of such waste. Some of the waste streams originating from U and Pu analysis contain components like sulphate and phosphate which interfere during chemical precipitation of alpha activity from the waste. Various chemical co-precipitation experiments were conducted based on alkaline precipitation. Reductive precipitation using sodium sulphite and Fe(II) was found to be promising and the same was suggested for plant scale treatment. (author)

Energy Technology Data Exchange (ETDEWEB)

The authors have studied in detail the transient enhanced diffusion observed during furnace or rapid-thermal-annealing of ion-implanted Si. The authors show that the effect originates in the trapping of Si atoms by dopant atoms during implantation, which are retained during solid-phase epitaxial (SPE) growth but released by subsequent annealing to cause a transient dopant precipitation or profile broadening. The interstitials condense to form a band of dislocation loops located at the peak of the dopant profile, which may be distinct from the band formed at the original amorphous/crystalline interface. The band can develop into a network and effectively getter the dopant. They discuss the conditions under which the various effects may or may not be observed, and discuss preliminary observations on As/sup +/ implanted Si. 12 references, 12 figures.

International Nuclear Information System (INIS)

The authors have studied in detail the transient enhanced diffusion observed during furnace or rapid-thermal-annealing of ion-implanted Si. The authors show that the effect originates in the trapping of Si atoms by dopant atoms during implantation, which are retained during solid-phase epitaxial (SPE) growth but released by subsequent annealing to cause a transient dopant precipitation or profile broadening. The interstitials condense to form a band of dislocation loops located at the peak of the dopant profile, which may be distinct from the band formed at the original amorphous/crystalline interface. The band can develop into a network and effectively getter the dopant. They discuss the conditions under which the various effects may or may not be observed, and discuss preliminary observations on As"+ implanted Si. 12 references, 12 figures.

British Library Electronic Table of Contents (United Kingdom)

Translating experimental findings into clinically effective therapies is one of the major bottlenecks of modern medicine. As this has been particularly true for cerebrovascular research, attention has turned to the quality and validity of experimental cerebrovascular studies. We set out to assess the study design, statistical analyses, and reporting of cerebrovascular research. We assessed all original articles published in the Journal of Cerebral Blood Flow and Metabolism during the year 2008 against a checklist designed to capture the key attributes relating to study design, statistical analyses, and reporting. A total of 156 original publications were included (animal, in vitro, human). Few studies reported a primary research hypothesis, statement of purpose, or measures to safeguard in...

British Library Electronic Table of Contents (United Kingdom)

This study uses data from a random sample of births in the Netherlands during the period 1850-1922 to examine the relationships between social class, social mobility and mortality at middle and old age. Population registers and personal cards covering the period from 1850 to 2004 for all Dutch provinces were used to reconstruct individual life histories of 14,900 births. For men we did not find an effect of the social class of origin (using two different SES-classifications) on mortality in age group 18 to 35. We also did not observe an effect of own social class on mortality after age 35. For women effects of social class of origin and social class of husband were generally absent as well. Our conclusion is that the standard ideas about the negative effects of processes of industrializati...

International Nuclear Information System (INIS)

The authors describe a new method which makes it possible to determine the radial distribution of the diffuse component of galactic gamma rays outside the solar circle. They use the observation that a good correlation exists between gamma-ray intensities and total column densities of the local interstellar gas and that the fractional column density of H_2<0.1 HI outside the solar circle. Thus the gamma-ray intensities are shown to be proportional to N(HI). The authors use the kinematics of the HI to determine the distances from which various fractions of the emission originate in the second and third galactic quadrants. Preliminary results of our analysis show that a significant flux of gamma rays originates from distances as large as 18 kpc from the galactic centre. (Auth.).

Energy Technology Data Exchange (ETDEWEB)

The original objective of our EMSP research was to design, develop, and test new in situ field permeameters for use in spatial variability studies of unsaturated hydraulic properties. We initially focused on activities related to development and testing of techniques for inverting permeameter data, development of numerical tools for data inversion, error evaluation for permeameter design, and permeameter component design. However, the results of error evaluations for permeameter design caused us to redirect our research, and reformulate our research objectives. We discovered that small, simple measurement errors lead to significant bias in the estimated hydraulic properties and their spatial statistics. Our reformulated research objective became the evaluation of the origin, impact and relevance of spatial bias in field-and laboratory-estimated unsaturate hydraulic properties, with an emphasis on spatial statistics and the resulting predictions ...

International Nuclear Information System (INIS)

Lead is contained as trace element in uranium ores and propagates throughout the production process to intermediate products like yellow cake or uranium oxide. The lead isotopes in such material originate from two sources: natural lead and radiogenic lead. The variability of the isotopic composition of lead in ores and yellow cakes was studied and the applicability of this parameter for nuclear forensic investigations was investigated. Furthermore, the chemical impurities contained in these materials were measured in order to identify characteristic differences between materials from different mines. For the samples investigated, it could be shown, that the lead isotopic composition varies largely from mine to mine and it may be used as one of the parameters to distinguish between materials of different origins. Some of the chemical impurities show a similar pattern and support the conclusions drawn from the lead isotope data. (orig.)

British Library Electronic Table of Contents (United Kingdom)

An ultrafine-grained ferrite/cementite (UGF/C) steel with a local high density of cementite particles was fabricated through caliber-warm-rolling followed by annealing and resulted in a bimodal-sized microstructure. The characteristic bimodal-sized microstructure was attributed to the original ferrite-pearlite structure and cementite spacing, and reflected the original ferrite-pearlite structure. The smaller-sized clusters corresponded to the former pearlite regions and the larger-sized clusters to the proeutectoid ferrite regions. The cementite particles naturally localized within the former pearlite region. Most of the ferrite coarsening did not occur until the cementite particle spacing reached a critical value. The UGF/C microstructure with a bimodal grain size showed a yield strength ...

International Nuclear Information System (INIS)

The reliability analysis of the Fast Flux Test Facility reactor shutdown system was reevaluated. Failure information based on five years of plant operating experience was used to verify original reliability numbers or to establish new ones. Also, system modifications made subsequent to performance of the original analysis were incorporated into the reevaluation. Reliability calculations and sensitivity analyses were performed using a commercially available spreadsheet on a personal computer. The spreadsheet was configured so that future failures could be tracked and compared with expected failures. A number of recommendations resulted from the reevaluation including both increased and decreased surveillance intervals. All recommendations were based on meeting or exceeding existing reliability goals. Considerable cost savings will be incurred upon implementation of the recommendations.

British Library Electronic Table of Contents (United Kingdom)

Poaching of wildlife animals for subsistence and commercial purposes has lead to population declines in Africa. In forensic cases, a need exists to identify the species of origin of carcasses, meat or blood. In the study presented here, the mitochondrial COI gene was sequenced to determine the species of unknown samples in three suspect South African forensic wildlife cases. In two cases the unknown samples were identified as originating from domestic cattle (Bos taurus) and in the third case the sample was identified as common reedbuck (Redunca arundinum). This is the first report of the COI sequence of common reedbuck. The study highlights the need for accurate wildlife reference material from each country in order to convict wildlife cases.

International Nuclear Information System (INIS)

Stenting for stenosis of the proximal vertebral artery (VA) is commonly performed via a femoral approach. However, iliofemoral occlusive disease such as arteriosclerosis obliterans sometimes prevents safe transfemoral access. In certain situations where both femoral access and ipsilateral brachial access are difficult because of a concomitant vascular diseases or particular anatomic setting, a contralateral brachial approach using the brachiobrachial pull-through technique may allow efficient and accurate stenting. A case of VA origin symptomatic stenosis successfully treated with stenting using the new pull-through technique from the contralateral brachial artery to the brachial artery on the affected side is described. (orig.)

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to encourage other libraries to provide access to their own original content via the web. By way of case study, the process of identifying materials, scanning them and creating web access to original content will be explored. Design/methodology/approach - To implement a book digitization project, three major things need to be present. The library must own material that was published prior to 1923, making them safely out of copyright in the USA; the library needs access to server space on the web and the library needs to own scanning equipment. Findings - This project demonstrated that a library does not necessarily need to obtain grant funding to begin an effective and successful digitization program. It also revealed a few things that would improve t...

International Nuclear Information System (INIS)

The interpretation of data from the nuclear forensic analysis of illicit nuclear material of unknown origin requires comparative data from samples of known origin. One way to provide such comparative data is to create a system of national nuclear forensics libraries, in which each participating country stores information about nuclear or other radioactive material that either resides in or was manufactured by that country. Such national libraries could provide an authoritative record of the material located in or produced by a particular country, and thus forms an essential prerequisite for a government to investigate illicit uses of nuclear or other radioactive material within its borders. We describe the concept of the national nuclear forensic library, recommendations for content and structure, and suggested querying methods for utilizing the information for addressing nuclear smuggling.

Energy Technology Data Exchange (ETDEWEB)

Volume one contains calculations for: embankment design--embankment material properties; Union Carbide site--bedrock contours; vicinity properties--origin of contamination; North Continent and Union Carbide sites contaminated materials--excavation quantities; and demolition debris--quantity estimate.

International Nuclear Information System (INIS)

The method of treatment of uteral cancer by the brake irradiation of 25 MeV betatrone using original devices which promote forming therapeutic figured bunches is presented. The binding of the protective blocks with a special adjusting frame within the aperture of the diaphragm provided for low relative entering dose which is the advantage of high energy irradiation bunch. The use of the forming devices makes it possible to practice individual treatment and decrease the levels of irradiation doses for intact organs and tissues.

UK PubMed Central (United Kingdom)

Lethal alleles of the Drosophila k43 gene result in small or missing imaginal discs, greatly reduced mitotic index, and fragmented and abnormally condensed chromosomes. A female-sterile...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The author recounts his experiences with insertion devices at the Stanford Synchrotron Radiation Laboratory. His first experiences with wigglers occured at the Cambridge Electron Accelerator, and was carried over to SSRL with the proposal for a six pole electromagnetic wiggler. Most modern undulators, and many wigglers are now designed around permanent magnets, and the origin of this transition at SSRL was rather fortuitous and humorous. It reflects some of the personality characteristics of Klaus Halbach.

British Library Electronic Table of Contents (United Kingdom)

The South China Sea is an area of disputes on sovereignty and resource jurisdiction claims. As one of the six claimants, the Republic of China (Taiwan) has played an important role not only because it is the originator of the U-shaped lines, but also it has continuously occupied the largest island, Tai-Ping-Dao, in the Spratlys. This article reviews the ROC's position on the issues through an exploration of its maritime claims to the territorial sea, continental shelf, and exclusive economic zone.

UK PubMed Central (United Kingdom)

The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1],...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Scientists often re-invent things that were long known. Here we review these activities as related to the mechanism of producing power law distributions, originally proposed in 1922 by Yule to explain experimental data on the sizes of biological genera, collected by Willis. We also review the history of re-invention of closely related branching processes, random graphs and coagulation models.

International Nuclear Information System (INIS)

Most diffusion phenomena in solids can be understood (or sometimes misunderstood) on a purely classical model. For light interstitials (hydrogen isotopes, the positive muon, and potentially He) there may be anomalous temperature dependences, and isotope effects, and anomalous response to electric fields and temperature gradients. Some of these anomalies are quantal in origin, and will be discussed. (author).

International Nuclear Information System (INIS)

This paper reviewed the current status of basic research in plant cell engineering, highlighted the application of embryo culture, double haploid (DH) technology, protoplast culture and somatic hybridization, somaclonal variation, rapid propagation, and bio-products production of plant-origin, and t he prospects. (authors)

International Nuclear Information System (INIS)

Some of the origins of corrosion encountered in the secondary side of pressurized water reactor steam generators are:-sludge accumulation (a mixture of metal oxides, primarily magnetite and copper) on tube sheet and attack of tube support plates by aggressive impurities leading to denting. Although Electricite de France has not suffered from these problems, it has developed a chemical cleaning process to dissolve corrosion products at both locations. (author).

UK PubMed Central (United Kingdom)

FSD-1, a designed small ultrafast folder with a ββα fold, has been actively studied in the last few years as a model system for studying protein folding mechanisms and for testing...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The aim of this paper is to provide a general view of wave energy resource assessment. First, a review of the origin of waves and the transformation they undergo as they propagate towards the coast through waters of decreasing depth is presented. Following this, the wave and wave-energy parameters and the statistics required for resource characterization are described. The various types of wave data and their usefulness for the present purposes are summarised. A common methodology for assessment of the wave energy resource is developed. Finally, a general description of the global open ocean resource is presented.

Energy Technology Data Exchange (ETDEWEB)

The effect of nuclear scattering on the suprathermal fusion probability of recoiling d-t nuclei under the impact of fusion products, a 14.1-MeV neutron and a 3.5 MeV alpha particle, in thick pellets is investigated with the help of the Boltzmann-Fokker-Planck equation. Although the suprathermal fusion from alpha-particle recoils is quite small, the one originating from neutron recoils is considerable.

Wastenet

... Further complications resulted from the requirement to use existing piled foundations and columns which had originally been designed to accommodate a six-storey office block. The hotel bedroom configurations and planning constraints were different from thos Related Services Structural Design Go View all Services Find Projects Sector All Sectors Buildings & Infrastructure Environment Natural Resources Transportation Sub Sector All Sub-Sectors Country All Countries Angola Antigua and Barbuda Argentina Australia Azerbaijan ...

Science.gov (United States)

The Multiple Address Processing Unit (MAPU) of the AN/TGC-26 tape relay center was originally designed to process ACP 127 message format. With the advent of JANAP 128 as a standardized message format a pressing need was felt for a MAPU modification to all...

British Library Electronic Table of Contents (United Kingdom)

In diabetes complications, CCN-2 (known originally as CTGF) has been implicated in diabetic nephropathy both as a marker and a mediator of disease. This commentary addresses CCN-2 in diabetic nephropathy, in the context of the recent publication of the first human study to inhibit CCN-2 bioactivity in diabetic kidney disease.

UK PubMed Central (United Kingdom)

The carcinogenicity database used for this paper originated in the late 1960s by the National Cancer Institute (NCI) and since 1978 has been continued and made more comprehensive by the National Toxicology...Full Text Available

International Nuclear Information System (INIS)

Nuclear material is either a product of technological processing of natural source material or it is entirely of anthropogenic origin. Consequently, nuclear material carries 'tool-marks' or 'fingerprints' of the process it was subjected to. Uranium fuels are examples of the first category, while plutonium belongs to the second category. The nature of these production processes is reflected in the elemental and isotopic composition of the material as well as in its microscopic and macroscopic appearance. All of these parameters can be determined using appropriate analytical techniques and they may result in important conclusions on the history and on the origin of the material. Therefore, they provide the most essential contribution to the prevention of future diversions of nuclear material from the same source. So far, essentially metallic impurities or light elements have been investigated for their potential in providing clues either on the ...

Wastenet

...and supply ensuring better food quality for the consumer developing safer and more flexible food production processes and technologies raw materials for food production; production and processing systems; by-products and wastes from fisheries and agro-industry; new food sources; packaging systems; quality monitoring; traceability Food contamination detecting and eliminating infectious and toxic agents throughout the food chain establishing the hazards and origins of food contaminants producing food more safely rapid ...

CERN Document Server

The periodic mode is analyzed together with two conventional boundary handling modes for particle swarm. By providing an infinite space that comprises periodic copies of original search space, it avoids possible disorganizing of particle swarm that is induced by the undesired mutations at the boundary. The results on benchmark functions show that particle swarm with periodic mode is capable of improving the search performance significantly, by compared with that of conventional modes and other algorithms.

Energy Technology Data Exchange (ETDEWEB)

The gastrin releasing peptide (GRP) receptor is becoming an increasingly attractive target for development of new radiolabeled peptides with diagnostic and therapeutic potential. The attractiveness of the GRP receptor as a target is based upon the functional expression of GRP receptors in several tumors of neuroendocrine origin including prostate, breast, and small cell lung cancer. This concise review outlines some of the efforts currently underway to develop new GRP receptor specific radiopharmaceuticals by employing a variety of radiometal chelation systems.