UK PubMed Central (United Kingdom)

Certain pathogenic species of Bacillus and Clostridium have developed unique methods for intoxicating cells that employ the classic enzymatic “A-B” paradigm for protein toxins. The binary...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Abstract The available surface equation of state for pure pulmonary surfactant monolayers is generalised to binary mixture monolayers by introducing a group of parameters, i(i-=-1-4) in the form of $x_{r}^{\\beta _{i} } $ to express the influence of the components in new mixing rules and a new factor, I $\\left[ { = \\left( {\\prod\\limits_{i = 1}^{4} {\\beta _{i} } } \\right)^{{\\raise0.5ex\\hbox{$\\scriptstyle 1$}\\kern-0.1em/\\kern-0.15em\\lower0.25ex\\hbox{$\\scriptstyle {4}$}}} } \\right]$, is defined to represent the interaction intensity between two different components. The ---A isotherms getting by the surface equations of state agree with the experimental data for protein-lipid binary monolayers, and the average deviation is about 11.41%. The result shows the order of the interaction intensity b...

Energy Technology Data Exchange (ETDEWEB)

The major problem of measurement of a power spectral density (PSD) distribution of surface heights with surface profilometers arises due to the unknown Modulation Transfer Function (MTF) of the instruments, which tends to distort the PSD at higher spatial frequencies. The special mathematical properties of binary pseudo-random patterns make them an ideal basis for developing MTF calibration test surfaces. Two-dimensional binary pseudo-random arrays (BPRAs) have been fabricated and used for the MTF calibration of the MicroMap{trademark}-570 interferometric microscope with all available objectives. An investigation into the effects of fabrication imperfections on the quality of the MTF calibration and a procedure for accounting for such imperfections are presented.

Energy Technology Data Exchange (ETDEWEB)

Synaptobrevin is a synaptic vesicle protein that has an essential role in exocytosis and forms the SNARE complex with syntaxin and SNAP-25. We have analyzed the structure of isolated synaptobrevin and its binary interaction with syntaxin using NMR spectroscopy. Our results demonstrate that isolated synaptobrevin is largely unfolded in solution. The entire SNARE motif of synaptobrevin is capable of interacting with the isolated C-terminal SNARE motif of syntaxin but only a few residues bind to the full-length cytoplasmic region of syntaxin. This result suggests an interaction between the N- and C-terminal regions of syntaxin that competes with core complex assembly.

UK PubMed Central (United Kingdom)

The protein databank (PDB) contains high quality structural data for computational structural biology investigations. We have earlier described a fast tool (the decomp_pdb tool) for identifying and...Full Text Available

UK PubMed Central (United Kingdom)

Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in storage organelles in the endosperm during seed maturation as a nitrogen source for germinating seedlings....Full Text Available

International Nuclear Information System (INIS)

Soybean is one of the most economical and nutritious food packed with basic nutrients that combat diseases stemming from mal- and under-nutrition. Despite its rich nutritional profile, use of soybean in food has been limited because soybean proteins are often associated with compounds, which could exert a negative impact on the nutritional quality of the protein. Trypsin inhibitor (TI) is one of the important anti-nutritional factors that exert negative effect by causing growth inhibition. Soybean cultivar VLS-2 was irradiated with 250 Gy gamma rays in a gamma cell (200) with 60Co source installed at BARC to induce mutations for low trypsin inhibitor content. Three mutants with lower levels of TI content were identified and can be utilized for developing elite varieties of soybean. (author)

Science.gov (United States)

Binary Star Evolution. Stars in binary systems evolve individually and together. " individually: evolution follows normal progression based on ...

British Library Electronic Table of Contents (United Kingdom)

Mycoplasma ovipneumoniae is associated with chronic non-progressive pneumonia of sheep and goats. As with many other mycoplasmas involved in animal diseases, protective immune responses have not been achieved with vaccines, even though antibody responses can be obtained. This study focuses on characterizing the interaction of M. ovipneumoniae with ovine PBMC using carboxy-fluorescein-succinimidyl-ester (CFSE) loading and flow cytometry to measure lymphoid cell division. M. ovipneumoniae induced a strong in vitro polyclonal suppression of CD4^+, CD8^+, and B blood lymphocyte subsets. The suppressive activity could be destroyed by heating to 60^oC, and partially impaired by formalin and binary ethyleneimine treatment that abolished its viability. The activity resided on the surface-exposed m...

British Library Electronic Table of Contents (United Kingdom)

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage protei...

Science.gov (United States)

Digital audio sampling is explained, and some of its implications in digital sound applications are discussed. Digital sound equipment is rapidly replacing analog recording devices as the state-of-the-art in audio technology. The philosophy of digital recording involves doing away with the continuously variable analog waveforms and turning the patterns into numbers. A digital recording device rapidly samples the incoming sounds, quantifying the signal into a series of numerical values (binary codes). Although digital sound eliminates many of the traditional analog problems, digital signal processing presents key problems in sampling rates and synchronization. Careful control is necessary to check signals through each step in the audio chain. The advantages of digital audio processing include increased signal-to-noise ratio, no flutter, transparent generation of copies, and sound manipulation. These benefits come with increased quality control ...

Energy Technology Data Exchange (ETDEWEB)

A plutonium gamma-ray analysis system that operates on MS-DOS-based computers has been developed for the International Atomic Energy Agency (IAEA) to perform in-field analysis of plutonium gamma-ray spectra for plutonium isotopics. The program titled IAEAPU consists of three separate applications: a data-transfer application for transferring spectral data from a CICERO multichannel analyzer to a binary data file, a data-analysis application to analyze plutonium gamma-ray spectra, for plutonium isotopic ratios and weight percents of total plutonium, and a data-quality assurance application to check spectral data for proper data-acquisition setup and performance. Volume 3 contains the software listings for these applications.

Science.gov (United States)

... CVn) consisting of a dM3 star and a cool white dwarf that must have evolved through the common-envelope stage of binary star evolution (Else van ...

J-STORE (Japan)

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CERN Document Server

We describe a method of determining the system parameters in non-eclipsing interacting binaries. We find that the extent to which an observer sees the shape of the Roche-lobe of the secondary star governs the amount of distortion of the absorption line profiles. The width and degree of asymmetry of the phase-resolved absorption line profiles show a characteristic shape, which depends primarily on the binary inclination and gravity darkening exponent. We show that, in principle, by obtaining high spectral and time resolution spectra of quiescent cataclysmic variables or low mass X-ray binaries in which the mass-losing star is visible, fitting the shape of absorption line profiles will allow one to determine not only the mass function of the binary, but also the binary inclination and hence the mass of the binary components.

International Nuclear Information System (INIS)

After presenting a general account of the observed global properties of single stars of low, intermediate, and high mass, together with their theoretical Hertzsprung-Russell diagram evolution, attention is given to the observed properties of various evolved close binaries and to an assessment of the value of comparisons between observation and crude theory in characterizing the physics of mass transfer within interacting binary systems. Detailed consideration is then undertaken of such topics as stellar evolution in globular clusters, interior star changes due to nucleosynthesis and mixing, asymptotic giant branch stars of intermediate mass, the response of white dwarfs in binary systems to mass accretion, and scenarios for binary star evolution tending toward close white dwarf pairs.

Science.gov (United States)

In this paper, necessary and sufficient conditions for successful detection of errors in a binary adder by any separate code are developed. The author demonstrates the existence of separate checking codes for addition modulo (2 sup n) ( n > or = 4) and mo...

CERN Document Server

We estimate binary compact object merger detection rates for LIGO, including the binaries formed in ellipticals long ago. Specifically, we convolve hundreds of model realizations of elliptical- and spiral-galaxy population syntheses with a model for elliptical- and spiral-galaxy star formation history as a function of redshift. Our results favor local merger rate densities of 4\\times 10^{-3} {Mpc}^{-3}{Myr}^{-1} for binary black holes (BH), 3\\times 10^{-2} {Mpc}^{-3}{Myr}^{-1} for binary neutron stars (NS), and 10^{-2} {Mpc}^{-3}{Myr}^{-1} for BH-NS binaries. Mergers in elliptical galaxies are a significant fraction of our total estimate for BH-BH and BH-NS detection rates; NS-NS detection rates are dominated by the contribution from spiral galaxies. Using only models that reproduce current observations of Galactic NS-NS binaries, we find slightly higher rates ...

CERN Document Server

The massive stars in the Galactic center inner arcsecond share analogous properties with the so-called Hot Jupiters. Most of these young stars have highly eccentric orbits, and were probably not formed in-situ. It has been proposed that these stars acquired their current orbits from the tidal disruption of compact massive binaries scattered toward the proximity of the central supermassive black hole. Assuming a binary star formed in a thin gaseous disk beyond 0.1 pc from the central object, we investigate the relevance of disk-satellite interactions to harden the binding energy of the binary, and to drive its inward migration. A massive, equal-mass binary star is found to become more tightly wound as it migrates inwards toward the central black hole. The migration timescale is very similar to that of a single-star satellite of the same mass. The binary's hardening is caused by the ...

Science.gov (United States)

Understanding the detailed mechanics of these systems and their place in the general picture of binary star evolution are the dual objectives of this ...

Science.gov (United States)

... Abstract : The inherent brittleness of the A-15 compounds, and the requirement for a filamentary morphology, led to a heavy reliance on a powder ...

CERN Document Server

The method of spectral disentangling has now created the opportunity for studying the chemical composition in previously inaccessible components of binary and multiple stars. This in turn makes it possible to trace their chemical evolution, a vital aspect in understanding the evolution of stellar systems. We review different ways to reconstruct individual spectra from eclipsing and non-eclipsing systems, and then concentrate on some recent applications to detached binaries with high-mass and intermediate-mass stars, and Algol-type mass-transfer systems.

CERN Document Server

Dusty primordial disks surrounding young low-mass stars are revealing tracers of stellar and planetary formation. The evolution and lifetime of these disks define the boundary conditions of the mechanisms of planet formation. Stellar companions, however, can significantly change this evolution through their tidal interactions. Stellar evolution and planet formation in binaries have to respond to an environment of truncated, quickly disappearing disks--very different compared to an isolated star environment. In order to investigate details of the influence of binarity on circumstellar disk evolution, we obtained adaptive optics supported near-infrared imaging and spectroscopy of the individual components of 22 low-mass binaries in the well-known Orion Nebula Cluster. Brackett gamma emission, which we detect in several systems, is used as a tracer for the presence of an active accretion disk around each binary component. We ...

DEFF Research Database (Denmark)

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition. The antisense line had a lower C-hordein level and down-regulated transcript encoding ...

DEFF Research Database (Denmark)

Udgivelsesdato: 2008/5

UK PubMed Central (United Kingdom)

The intracellular concentration of protein may be as high as 400 mg per ml; thus it seems inevitable that within the cell, numerous protein-protein contacts are constantly occurring. A basic biochemical...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Large scale production of monoclonal antibodies has been accomplished using bioreactors with different length to diameter ratios, and diverse impeller and sparger designs. The differences in these physical attributes often result in dissimilar mass transfer, mechanical stresses due to turbulence and mixing inside the bioreactor that may lead to disparities in cell growth and antibody production. A rational analysis of impeller design parameters on cell growth, protein expression levels and subsequent antibody production is needed to understand such differences. The purpose of this study was to examine the impact of Rushton turbine and marine impeller designs on Chinese hamster ovary (CHO) cell growth and metabolism, and antibody production and quality. Experiments to evaluate mass transfer...

International Nuclear Information System (INIS)

Spices and packaging materials were exposed to gamma irradiation at a dose of 10 KGy. Luncheon was prepared with irradiated or non-irradiated spices. Prepared luncheon was packaged in irradiated or non-irradiated packaging materials. Packaged luncheon was treated with 2 KGy. Treated and untreated packaged luncheon were kept in a refrigerator (1-4 Centigrade) for 12 months. Microbiological, nutritive and chemical characteristics of luncheon were evaluated after processing and during storage; whereas, sensory quality was evaluated only after irradiation. Gamma irradiation decreased the microorganisms counts of spices, packaging materials and packed luncheon and increased the shelf-life of packaged luncheon products. No major differences in moisture, protein, fat, ph value, total acidity, lipid peroxide and volatile basic nitrogen were observed due to irradiation. Sensory evaluation showed that all the combination treated luncheon were acceptable ...

International Nuclear Information System (INIS)

Microdamage naturally occurs in bone tissue as a result of cyclic loading placed on the body from normal daily activities. While it is usually repaired through the bone turnover process, accumulation of microdamage may result in reduced bone quality and increased fracture risk. It is unclear whether certain areas of bone are more susceptible to microdamage than others due to compositional differences. This study examines whether areas of microdamaged bone are chemically different than undamaged areas of bone. Bone samples (L3 vertebrae) were harvested from 15 dogs. Samples were stained with basic fuchsin, embedded in poly-methylmethacrylate, and cut into 5-(micro)m-thick sections. Fuchsin staining was used to identify regions of microdamage, and synchrotron infrared microspectroscopic imaging was used to determine the local bone composition. Results showed that microdamaged areas of bone were chemically different than the surrounding undamaged areas. Specifically, ...

Science.gov (United States)

Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding protein that is

UK PubMed Central (United Kingdom)

Many mutations associated with retinal degeneration lead to the production of misfolded proteins by cells of the retina. Emerging evidence suggests that these abnormal proteins cause cell death...Full Text Available

CERN Document Server

We highlight the role of the light elements (Li, Be, B) in the evolution of massive single and binary stars, which is largely restricted to a diagnostic value, and foremost so for the element boron. However, we show that the boron surface abundance in massive early type stars contains key information about their foregoing evolution which is not obtainable otherwise. In particular, it allows to constrain internal mixing processes and potential previous mass transfer event for binary stars (even if the companion has disappeared). It may also help solving the mystery of the slowly rotating nitrogen-rich massive main sequence stars.

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The common envelope phase of binary star evolution plays a central role in many evolutionary pathways leading to the formation of compact objects in short period systems. Using three dimensional hydrodynamical computations, we review the major features of this evolutionary phase, focusing on the conditions that lead to the successful ejection of the envelope and, hence, survival of the system as a post common envelope binary. Future hydrodynamical calculations at high spatial resolution are required to delineate the regime in parameter space for which systems survive as compact binary systems from those for which the two components of the system merge into a single rapidly rotating star. Recent algorithmic developments will facilitate the attainment of this goal.

UK PubMed Central (United Kingdom)

A binary mixture of imipramine HCl and chlordiazepoxide was determined by three different spectrophotometric methods. The first method involved determination of imipramine HCl and chlordiazepoxide using...Full Text Available

Science.gov (United States)

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster ...

Science.gov (United States)

Aug 21, 2003 ... Her research interests have included hot stars, colliding stellar winds, binary star evolution and evolved stellar companions. ...

Science.gov (United States)

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster structure ...

Science.gov (United States)

... each star goes. The results of (1) are compared with predictions based on (2) and on single star evolution theory to discover the various stages which occur. ...

Energy Technology Data Exchange (ETDEWEB)

The X-ray properties of the main sequence Be stars conclude that they are a widely separated binary system containing an accreting neutron star.

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

Science.gov (United States)

PMID:20880255

KoreaScience (Korea)

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Energy Technology Data Exchange (ETDEWEB)

The Gibbs energy of mixing in liquid phase and the Gibbs energy of formation of various compounds in a binary Pu-Cd system were re-optimized by applying both recent and earlier experimental data. Those of the binary Pu-Bi and U-Bi systems were newly optimized. The results were summarized in a database and applied to the preliminary prediction of multi-solute systems in liquid Cd or Bi solvent. (author)

International Nuclear Information System (INIS)

These proceedings represent papers presented at the Astrophysics Conference in Maryland, USA. The topic of the Conference was the evolution of x-ray binaries and the papers encompass a wide range of subjects on x-ray astronomy. There were one hundred eighteen papers presented at the Conference and out of these three have been abstracted for the Energy Science and Technology database.

International Nuclear Information System (INIS)

The rapid circularization and synchronization of the stellar components in an eccentric binary system at the onset of Roche lobe overflow is a fundamental assumption common to all binary stellar evolution and population synthesis codes, even though the validity of this assumption is questionable both theoretically and observationally. Here we calculate the evolution of the orbital elements of an eccentric binary through the direct three-body integration of a massive particle ejected through the inner Lagrangian point of the donor star at periastron. The trajectory of this particle leads to three possible outcomes: direct accretion onto the companion star within a single orbit, self-accretion back onto the donor star within a single orbit, or a quasi-periodic orbit around the companion star, possibly leading to the formation of a disk. We calculate the secular evolution of the binary orbit in the first ...

Energy Technology Data Exchange (ETDEWEB)

A dual-boiling isobutane cycle was selected for the present 5-megawatt (5MW) Raft River Pilot Plant to utilize the lower-temperature geothermal resources (near 300F). This study represents a second effort directed toward the design of an improved binary geothermal electric plant suitable for utilization of the lower temperature resources. 7 refs.

CERN Document Server

Empirical birthrate estimates for pulsar binaries depend on the fraction of sky subtended by the pulsar beam: the pulsar beaming fraction. This fraction depends on both the pulsar's opening angle and the misalignment angle between its spin and magnetic axes. Previous estimates use the average value for only two pulsars, i.e. PSRs B1913+16 and B1534+12. We explore how birthrate predictions depend on assumptions about opening angle and alignment, using empirically-motivated distributions to define an effective beaming correction factor, f_{b,eff}. For most known pulsars, we expect f_{b,eff} to be less than 6. We also calculate f_{b,eff} for PSRs J0737-3039A and J1141-6545, applying the currently available constraints for their beam geometry. Our median posterior birthrate predictions for tight PSR-NS binaries, wide PSR-NS binaries, and tight PSR-WD binaries are 89/Myr, 0.84/Myr, and 34/Myr, respectively. ...

CERN Document Server

We present new results from accurate and fully general-relativistic simulations of the coalescence of unmagnetized binary neutron stars with various mass ratios. The evolution of the stars is followed through the inspiral phase, the merger and prompt collapse to a black hole, up until the appearance of a thick accretion disk, which is studied as it enters and remains in a regime of quasi-steady accretion. Although a simple ideal-fluid equation of state with \\Gamma=2 is used, this work presents a systematic study within a fully general relativistic framework of the properties of the resulting black-hole--torus system produced by the merger of unequal-mass binaries. More specifically, we show that: (1) The mass of the torus increases considerably with the mass asymmetry and equal-mass binaries do not produce significant tori if they have a total baryonic mass M_tot >~ 3.7 M_sun; (2) Tori with masses M_tor ~ 0.2 M_sun are ...

British Library Electronic Table of Contents (United Kingdom)

Dutch-style fermented sausages were manufactured with 15% and 30% pork back-fat substitution by pure or commercial encapsulated fish oil, either added as such or as pre-emulsified mixture with soy protein isolate. Adding commercial encapsulated fish oil was the most important factor influencing the chemical composition. The fat content was not significantly different between products (p>0.05). The n-6/n-3 ratio decreased from 8.49 in controls to 0.90-2.47 in modified products. Lipid oxidation parameters (propanal and hexanal) showed much higher values for sausages with pure fish oil than for products with encapsulated oil. For the latter, lipid oxidation was similar to controls. Products with encapsulated or pre-emulsified oil were significantly firmer than products from other treatments i...

International Nuclear Information System (INIS)

This study was carried out to evaluate the effects of irradiation (gamma irradiation at dose levels of 0.0, 2.5, 5.0, 10.0 or 20.0 kGy, He-Ne and their combination) on nutritive characteristics of Vicia faba L. seeds (Giza 843). Analyses included levels of anti-nutrients (total phenolic compounds, tannins, phytic acid, trypsin inhibitor and vicine), chemical composition (sugars, crude proteins, fat, dry matter and ash) and fatty acids profile. All treatments caused significant (P<0.05) decreases in the antinutrational factors under investigation. The reduction in the content of tannins and phenolic compounds were more pronounced with He-Ne alone or He-Ne in combination with gamma irradiation. At dose levels 10.0 and 20.0 kGy without or with combination of He-Ne were more efficiency in the reduction of phytic acid content by 24.3, 32.8, 34.5 and 45.5 %, respectively. Also, for trypsin inhibitor activity, the maximum inhibition was observed with the treatment of ...

British Library Electronic Table of Contents (United Kingdom)

Proteins aggregated into spherulite structures of amyloid fibrils have been observed in patients with certain brain diseases such as Alzheimers and Parkinsons. The conditions under which these protein spherulites form and grow are not currently known. In order to illuminate the role of environmental factors on protein spherulites, this research aims to explore the kinetics and mechanisms of spherulite formation and growth, as monitored by optical microscopy, in a range of salt concentrations, and initial protein concentrations for two model proteins: bovine b-lactoglobulin and insulin. These two proteins are significantly different in their size and fibril growth rate, but both of these proteins have been shown previously to form amyloid fibrils and spherulites under low pH conditions. The...

Science.gov (United States)

Nanosized particles are important because of their unique properties, different from the bulk, which leads to their enhanced catalytic, photocatalytic and electronic properties. This work has dealt with three different nanoparticle systems in the context of three different aspects of nanoparticle properties: (a) photocatalytis (TiO2/metal) system, (b) luminescence (CdSe) (c) alloying (Pt-Ag and Pd-Ag). The initial photocatalytic enhancement obtained by adding noble metal on semiconductor nanoparticles, degrades as fast as in 15 minutes and questions their long-term performance. XANES measurements on such irradiated systems like TiO2/Au, TiO2/Pt, TiO2/Ir indicates a positive oxidation state of these noble metals which renders them as recombination centers for photo-excited electrons and explains the decreased photocurrent. The oxidation is caused by holes. The EXAFS results also indicate a change of the interfacial structure under the effect of UV-irradiation, thus affecting the ...

British Library Electronic Table of Contents (United Kingdom)

In this paper we focus on appearance features describing the manual component of Sign Language particularly the Local Binary Patterns. We compare the performance of these features with geometric moments describing the trajectory and shape of hands. Since the non-manual component is also very important for sign recognition we localize facial landmarks via Active Shape Model combined with Landmark detector that increases the robustness of model fitting. We test the recognition performance of individual features and their combinations on a database consisting of 11 signers and 23 signs with several repetitions. Local Binary Patterns outperform the geometric moments. When the features are combined we achieve a recognition rate up to 99.75% for signer dependent tests and 57.54% for signer indep...

Energy Technology Data Exchange (ETDEWEB)

The experimental densities, {rho} for binary mixtures of (N-acetylmorpholine + benzene, +toluene, +ethylbenzene, and +mesitylene) have been measured at temperatures from (293.15 to 343.15) K and atmospheric pressure over the whole mole fraction range. From these results, excess molar volumes V{sup E} have been calculated and fitted to the Redlich-Kister polynomial equation. The V{sup E} values are negative over the whole mole fraction range and at all temperatures. Thermal expansion coefficients {alpha}{sub i}, apparent molar volumes V{phi}{sub i}, and partial molar volumes at infinite dilution V{sub i}{sup {infinity}} have been calculated. The variation of these properties with composition and temperature of the binary mixtures are discussed in terms of molecular interactions.

UK PubMed Central (United Kingdom)

Selection of the appropriate radiation quality is an important aspect of optimisation for every clinical imaging task in radiology, since it affects both image quality and patient dose. Spreadsheet...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

UK PubMed Central (United Kingdom)

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...Full Text Available

UK PubMed Central (United Kingdom)

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

UK PubMed Central (United Kingdom)

The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis. Of the 261 proteins scored, 68 (26%) show dramatic changes in...Full Text Available

UK PubMed Central (United Kingdom)

A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands...Full Text Available

UK PubMed Central (United Kingdom)

We have used the two-electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK1 channels by protein-tyrosine phosphatase (PTP) and protein-tyrosine...Full Text Available

UK PubMed Central (United Kingdom)

A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts...Full Text Available

UK PubMed Central (United Kingdom)

Protein modifications such as phosphorylation are often studied by two-dimensional gel electrophoresis since the perturbation in the protein’s pI value is readily detected by this method....Full Text Available

UK PubMed Central (United Kingdom)

The Protein Data Bank in Europe (PDBe; pdbe.org) is actively involved in managing the international archive of biomacromolecular structure data as one of the partners in the Worldwide Protein Data Bank...Full Text Available

UK PubMed Central (United Kingdom)

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this...Full Text Available

UK PubMed Central (United Kingdom)

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

UK PubMed Central (United Kingdom)

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

UK PubMed Central (United Kingdom)

Ethylene rapidly and transiently up-regulates the activity of several monomeric GTP-binding proteins (monomeric G proteins) in leaves of Arabidopsis as determined by two-dimensional gel electrophoresis...Full Text Available

UK PubMed Central (United Kingdom)

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

UK PubMed Central (United Kingdom)

Tooth enamel biomineralization is mediated by enamel proteins synthesized by ameloblast cells. Two classes of proteins have been described: enamelins and amelogenins. In lower vertebrates the absence...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe fast growing Protein Data Bank contains the three-dimensional description of more than 45000 protein- and nucleic-acid structures today. The large majority of the data...Full Text Available

UK PubMed Central (United Kingdom)

Alpha-hemoglobin-stabilizing protein (AHSP) is an erythroid-specific protein that acts as a molecular chaperone for the free α chains of hemoglobin. Evidence strongly suggests...Full Text Available

International Nuclear Information System (INIS)

The variation in the superconducting properties of various binary alloys of transition metal-germanium systems was surveyed by studying sputter deposited samples prepared under various conditions. The primary interest has been to study the formation of the stoichiometric A-15 compounds T_3Ge.

Energy Technology Data Exchange (ETDEWEB)

It is shown that a natural consequence of the binary pulsar's evolution is a neutron star collision. Such a collision is expected to eject neutron-rich matter of an r-process character. Taking reasonable estimates for the number of such events over the history of the galaxy, it may be that they account for all of the r-process nuclei.

Energy Technology Data Exchange (ETDEWEB)

An analytic model of magnetic torques applied to an accreting neutron star is employed to evaluate the magnetic dipole moments of x-ray pulsars. A new type of close binary system containing a neutron star is suggested.

Energy Technology Data Exchange (ETDEWEB)

The Heat Cycle Research Facility (HCRF) is an experimental binary-cycle facility used to investigate different concepts and/or components for generating electrical power from a geothermal resource. This report briefly desc

CERN Document Server

We derive spin-orbit coupling effects on the gravitational field and equations of motion of compact binaries in the 2.5 post-Newtonian approximation to general relativity, one PN order beyond where spin effects first appear. Our method is based on that of Blanchet, Faye, and Ponsot, who use a post-Newtonian metric valid for general (continuous) fluids and represent pointlike compact objects with a delta-function stress-energy tensor, regularizing divergent terms by taking the Hadamard finite part. To obtain post-Newtonian spin effects, we use a different delta-function stress-energy tensor introduced by Bailey and Israel. In a future paper we will use the 2.5PN equations of motion for spinning bodies to derive the gravitational-wave luminosity and phase evolution of binary inspirals, which will be useful in constructing matched filters for signal analysis. The gravitational field derived here may help in posing initial data for numerical ...

CERN Document Server

Binary mass transfer via Roche-lobe overflow (RLOF) is a key channel for producing stripped-envelope Wolf-Rayet (WR) stars and may be critical to account for SN Ib/c progenitors. RY Scuti is an extremely rare example of a massive binary star caught in this brief but important phase. Its toroidal nebula indicates equatorial mass loss during RLOF, while the mass-gaining star is apparently embedded in an opaque accretion disk. RY Scuti's toroidal nebula has two components: an inner ionised double-ring system, and an outer dust torus that is twice the size of the ionised rings. We present two epochs of Lband Keck NGS-AO images of the dust torus, plus three epochs of HST images of the ionised gas rings. Proper motions show that the inner ionised rings and the outer dust torus came from two separate ejection events roughly 130 and 250 yr ago. This suggests that RLOF in massive contact binaries can be accompanied by eruptive and ...

UK PubMed Central (United Kingdom)

The receptor-associated protein (RAP) is a molecular chaperone that binds tightly to certain newly synthesized LDL receptor family members in the endoplasmic reticulum (ER) and facilitates their delivery...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

UK PubMed Central (United Kingdom)

We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter...Full Text Available

Science.gov (United States)

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

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Energy Technology Data Exchange (ETDEWEB)

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Energy Technology Data Exchange (ETDEWEB)

Results of analytical study of early stages of a single star evolution are used investigation of the rotational fragmentation of the collapsing gas-dust cloud which leads to formation of two types of binaries. Wide ..cap alpha..-systems (Psub(orb) > or approximately 100 yrs) with usually unequal masses of components are formed before the formation of gas-dust core in hydrostatical equilibrium. Close ..beta..-systems (Psub(orb) < or approximation 100 yrs) with usually nearly equal masses of components are formed in the course of the collapse of gas-dust core.

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We consider propagation of relativistic heavy nuclei injected by a young pulsar into the radiation field of a massive companion. If the binary system (BS) is compact enough, then the nuclei suffer multiple photodisintegrations in collisions with thermal photons coming from the massive star (MS). Due to the propagation effects of charged particles in the magnetic field of the MS some hadrons can impinge onto the MS surface at large angles. We calculate the fluxes of produced neutrinos as a function of the viewing angle measured from the plane of the BS. It is found that significant fluxes of neutrinos should be also expected in the case of non-eclipsing BSs.

Science.gov (United States)

This chapter reports recent advances in the statistical learning literature that may be of interest for biometrics. In particular we discuss two different algorithmic settings, binary classification and multi-task learning, and analyze the two closely related problems of feature selection and feature learning. In the binary case the theoretical and algorithmic advances to feature selection are applied to solve face detection and face authentication problems. In the multi-task case we show how the data structure described by a group of features common to the various tasks can be effectively learned, and then we discuss how this approach could be used to address face recognition.

Energy Technology Data Exchange (ETDEWEB)

A special study was conducted to investigate the influences of minimum approach temperature differences occurring in supercritical-heater/vaporizer and evaporative-condenser heat rejection systems on geothermal-electric binary power plant performance and cost of electricity. For the systems investigated optimum pinch points for minimizing cost of electricity were estimated to range from 5 to 7/sup 0/F for the heater vaporizer. The minimum approach of condensing temperature to wet-bulb temperature for evaporative condensers was estimated to be about 30/sup 0/F in order to achieve the lowest cost of electricity.

CERN Document Server

We study a percolation process on the planted binary tree, where clusters freeze as soon as they become larger than some fixed parameter N. We show that as N goes to infinity, the process converges in some sense to the frozen percolation process introduced by Aldous. In particular, our results show that the asymptotic behaviour differs substantially from that on the square lattice, on which a similar process has been studied recently by van den Berg, de Lima and Nolin.

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This is the first time that the adsorption of binary mixtures of polycyclic aromatic hydrocarbons (PAHs) has been studied. This study was carried out at laboratory scale in an experimental apparatus specially designed for this aim: with gas-phase fluorescence, a new detection procedure was used. Experimental conditions, mainly in terms of temperature (150C) and contaminant concentration (approximately 1 ppmv), close to the ones observed in energy generation systems, were applied. The PAH adsorption process interpretation was carried out by recording the experimentally obtained breakthrough curves. After the detection parameter optimization, the influence of adsorbate characteristics in the hot gas cleaning of PAH was studied. In this system, the adsorption of 10 binary mixtures of five PAH (naphthalene (Np), fluorene (Fu), phenanthrene (Phe), fluoranthene (Fl), and pyrene (Py)) on an activated carbon was investigated. It was found that the ...

UK PubMed Central (United Kingdom)

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

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Biological cells of the nitrogen fixating bacteria Xanthobacter autotrophicus GZ 29 have been cultivated with 57-Fe, and from the nitrogenase of these cells the 57-Fe marked molybdenum-iron-protein is extracted. The Moessbauer spectra of this enzyme show that the structure of the iron cluster is similar to the structure in the molybdenum-iron-proteins of Azotobacter vinelandii, Klebsiella pneumoniae or Clostridium pasteurianum.

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UK PubMed Central (United Kingdom)

Protein bodies within the endosperm of castor bean (Ricinus communis L. cv. Hale) seeds arise from numerous small vacuoles which progressively become filled with storage protein, of...Full Text Available

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International Nuclear Information System (INIS)

In a series of experiments carried out on young and adult rabbits the effect of isocaloric low protein diets containing 4% or 8% protein compared with a diet containing 21% protein on Obeliscoides cuniculi infection was studied. The pathogenesis, resistance and local immunity were assessed after single infections with 10,000 larvae or reinfection with 5000 larvae. Live weight gain was reduced in young and adult rabbits fed the low protein diets, but the establishment of parasites was not substantially influenced by protein deprivation. However, development of worms in the histotrophic phase and parasite fecundity were impaired in association with the low protein diet. Moreover, mild anaemia as well as changes in the mucosal immune response as a result of infection were related to the level of dietary protein. (author). 30 refs, 6 figs, 5 ...

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TC inhibits protein synthesis in E. coli by interfering with aminoacyl-tRNA binding to the ribosomal A site, and there is strong evidence that such inhibition results from TC binding to a high affinity site on the 30S subunit. The SPORE approach has been used to define those 30S proteins that are potentially important for high affinity TC binding, measured as the (/sup 3/H)-TC co-sedimenting with the reconstitution particle through a sucrose density gradient. Reverse phase-HPLC has been used both to prepare ribosomal proteins and to analyze the protein content of reconstituted particles. The results obtained so far show that protein S7, as well as some proteins linked to S7 in the 30S assembly map, are important for TC binding, whereas other ribosomal proteins are not. These results are in very good accord with their earlier photoaffinity ...

International Nuclear Information System (INIS)

In Hungary, legislation for nuclear power plants was developed at the end of the 1970s, among which is the quality assurance code for nuclear power plants. Hungarian practice is presented, including discussion of the requirements for quality assurance, qualification of the suppliers and inspection practices. The general requirements of quality assurance in the course of construction of a nuclear power plant are presented: quality assurance of technological equipment, fuel, electrical equipment, automatic instrumentations, building structures and technology.

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Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd ...

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Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

International Nuclear Information System (INIS)

The research work carried out on mutation breeding in Egypt during the 1980s is summarized. Several mutations have been developed in bread wheat, maize, rice and barley. A higher yield, tolerance to salinity, shorter types and earliness were obtained after use of different mutagens and growth regulators. Great attention has been paid to the fababean and chickpea, particularly in improving their quality and quantity of protein, and their resistance to insect weevils such as Callosobruchus sp. Tolerance or resistance to broom rape has also been reported. Various grain legumes such as lentil, pea, cowpea, bean, fenugreek and lupin received some attention. Mutation work on fibre crops such as cotton, kenaf and flax has led to some promising results. Zero type, glandless and early maturing mutants were obtained in cotton, and early flowering, high yielding (fibre or oil) mutants in flax. Some attention has been given to oil crops such as sesame, ...

CERN Document Server

We present the results of three-dimensional hydrodynamical simulations of the final stages of inspiral in a black hole-neutron star binary, when the separation is comparable to the stellar radius. We use a Newtonian Smooth Particle Hydrodynamics (SPH) code to model the evolution of the system, and take the neutron star to be a polytrope with a soft (adiabatic index G=2 and G=5/3) equation of state and the black hole to be a Newtonian point mass. The only non-Newtonian effect we include is a gravitational radiation back reaction force, computed in the quadrupole approximation for point masses. We use irrotational binaries as initial conditions for our dynamical simulations, which are begun when the system is on the verge of initiating mass transfer and followed for approximately 23 ms. For all the cases studied we find that the star is disrupted on a dynamical time-scale, and forms a massive (the disc mass is approximately 0.2 solar masses) ...

CERN Document Server

To detect the gravitational-wave signal from binary neutron stars and extract information about the equation of state of matter at nuclear density, it is necessary to match the signal with a bank of accurate templates. We have performed the longest (to date) general-relativistic simulations of binary neutron stars with different compactnesses and used them to constrain a tidal extension of the effective-one-body model so that it reproduces the numerical waveforms accurately and essentially up to the merger. The typical errors in the phase over the $\\simeq 22$ gravitational-wave cycles are $\\Delta \\phi\\simeq \\pm 0.24$ rad, thus with relative phase errors $\\Delta \\phi/\\phi \\simeq 0.2%$. We also show that with a single choice of parameters, the effective-one-body approach is able to reproduce all of the numerically-computed phase evolutions, in contrast with what found when adopting a tidally corrected post-Newtonian Taylor-T4 expansion.

International Nuclear Information System (INIS)

We have developed a new method for calculating common envelope (CE) events based on explicit consideration of the donor star's structural response to adiabatic mass loss. In contrast to existing CE prescriptions, which specify a priori the donor's remnant mass, we determine this quantity self-consistently and find that it depends on binary and CE parameters. This aspect of our model is particularly important to realistic modeling for upper main-sequence star donors without strongly degenerate cores (and hence without a clear core/envelope boundary). We illustrate the central features of our method by considering CE events involving 10 M_s_u_n donors on or before their red giant branch. For such donors, the remnant core mass can be as much as 30% larger than the star's He-core mass. Applied across a population of such binaries, our methodology results in a significantly broader remnant mass and final orbital separation distribution and a 20% ...

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A general method for stable-isotope labeling of large proteins is introduced and applied for studies of the E. coli GroE chaperone proteins by solution NMR. In addition to enabling the residue-specific {sup 15}N-labeling of proteins on a highly deuterated background, it is also an efficient approach for uniform labeling. The method meets the requirements of high-level deuteration, minimal cross-labeling and high protein yield, which are crucial for NMR studies of structures with sizes above 150 kDa. The results obtained with the new protocol are compared to other strategies for protein labeling, and evaluated with regard to the influence of external factors on the resulting isotope labeling patterns. Applications with the GroE system show that these strategies are efficient tools for studies of structure, dynamics and intermolecular interactions in large supramolecular complexes, ...

CERN Document Server

Proteins are polymers of amino acids. These macromolecules are synthesized by intracellular machines called {\\it ribosome}. Although, traditionally, the experimental investigation of protein synthesis has been an active area of research in molecular cell biology, important quantitative models of this phenomenon have been reported mostly in the research journals devoted to statistical physics and related interdisciplinary topics. From the perspective of a physicist, protein synthesis is a phenomenon of {\\it classical transport of interacting ribosomes on a messenger RNA (mRNA) template} that dictates the sequence of the amino acids on the protein. Here we bring this frontier area of contemporary research into the classroom by appropriate simplification of the models and methods. In particular, we develope a simple toy model and analyze it by some elementary techniques of non-equilibrium statistical ...

British Library Electronic Table of Contents (United Kingdom)

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50??g/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30?min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

UK PubMed Central (United Kingdom)

BackgroundThe aim of this study is to validate the questionnaire ECOS-16 (Assessment of health related quality of life in osteoporosis) for the evaluation of health related quality...Full Text Available

UK PubMed Central (United Kingdom)

Quality reporting tools, integrated with ambulatory electronic health records, can help clinicians and administrators understand performance, manage populations, and improve quality. Report Central...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The variation of surface composition and sintering of the binary Pd{sub 7}Ag{sub 3} nanoparticles prepared by chemical reduction were studied. The composition of the as-prepared Pd{sub 7}Ag{sub 3} nanoparticles across the diameter is inhomogeneous due to different reduction potentials of Pd and Ag. Upon heating, Ag will migrate to the surface because of its lower surface energy. Surface modification results in variation of surface composition during the sintering process. Stearic acid acts as a grain growth inhibitor. The migration of Ag to the surface is suppressed and the sintering is retarded. Modification by polyethylene glycol leads to more migration of Ag atoms from the core to the surface. This makes the nanoparticles easier to sinter.

International Nuclear Information System (INIS)

Molar excess volumes, VE and molar excess enthalpies, HE of 1,3-dioxolane (D) (i) + propan-1-ol, 1,3-dioxolane (i) + butan-1-ol and 1,3-dioxolane (i) + butan-2-ol (j) binary mixtures have been measured as a function of composition at 308.15 K. The analysis of VE data by graph, theoretical approach reveals that while propan-1-ol, butan-1-ol exist as associated entities in the pure state; 1,3-dioxolane and butanol exist as monomers in their pure state. These (i+j) binary mixtures are characterized by interactions between ethereal oxygen atom of D(i) and hydrogen atom of alkanols. The IR studies lend additional support to the proposed structure of molecular entitles in these mixtures. The energetics of the mixtures have also been studied.

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In the first paper of this series devoted to a structural analysis of a binary metallic glass model, we study a Pd/sub 80/Si/sub 20/ sample obtained by numerical relaxation. We discuss the reproducibility of the method and make a comparison with the experimental interference functions. Then we undertake a microscopic structural analysis from several point of view: we first study the number of neighbours of each type for each type of atoms; secondly, we analyse the structure by means of the radical plane method; at last, we show that it is possible to generalize the five fundamental characteristic units introduced by Bernal, so that we can define the environment of any Si atom without any ambiguity. All these methods reveal a certain tendency towards a prismatic environment for the metalloids.

CERN Document Server

The detection and estimation of gravitational wave (GW) signals belonging to a parameterized family of waveforms requires, in general, the numerical maximization of a data-dependent function of the signal parameters. Due to noise in the data, the function to be maximized is often highly multi-modal with numerous local maxima. Searching for the global maximum then becomes computationally expensive, which in turn can limit the scientific scope of the search. Stochastic optimization is one possible approach to reducing computational costs in such applications. We report results from a first investigation of the Particle Swarm Optimization (PSO) method in this context. The method is applied to a testbed motivated by the problem of detection and estimation of a binary inspiral signal. Our results show that PSO works well in the presence of high multi-modality, making it a viable candidate method for further applications in GW data analysis.

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The biosorption of lead, copper and zinc ions on Rhizopus arrhizus has been studied for three single-component and two binary systems. The equilibrium data have been analysed using the Freundlich adsorption model. The characteristic parameters for the Freundlich adsorption model have been determined and the competition coefficients for the competitive biosorption of Pb(II)-Cu(II) at pH 4.0 and 5.0, and Pb(II)-Zn(II) at pH 5.0 have been calcualted. For the individual single-component isotherms, lead has the highest biosorption capacity followed by copper, then zinc. The capacity of lead in the two binary systems is always significantly greater than those of the other metal ions, in agreement with the single-component data. Only a partial selectivity for copper ions has been obtained at pH 4.0. (orig.)

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An experimental and theoretical study of heat and mass transfer analogy and a comparison of that to a binary liquid mixture evaporation is presented. Common organic solvents, ethanol and n-heptane, were used to form an alcohol - hydrocarbon mixture. Studies were carried out in a horizontal rectangular channel having air flow velocities of 0.2 - 0.9 m/s. Heat transfer coefficients were measured with a copper plate resistor and mass transfer coefficients with a square pool. The heat and mass transfer analogy is presented for a system having two evaporating compounds with a fixed value of air flow and verified by measuring and comparing mass transfer coefficients for distilled water with air flow velocities of 0.2 - 0.9 m/s. An illustrative example of the use of the theory for industrial ventilation is presented. (author)

Energy Technology Data Exchange (ETDEWEB)

The Heat Cycle Research Program, which is conducted for the Department of Energy, has as its objective the development of the technology for effecting the improved utilization of moderate temperature geothermal resources. The current testing involves the investigation of binary power cycle performance utilizing mixtures of non-adjacent hydrocarbons as the working fluids, with supercritical vaporization and in-tube condensation of the working fluid. The utilization of these concepts verified here will improve the net geofluid effectiveness (net watt hours plant output per pound of geofluid) about 20% over that of a conventional binary power plant. The major effect in this improvement is the ability to achieve integral, countercurrent condensation. Results are presented for the recent testing including those tests examining the performance of the countercurrent condenser at different tube inclinations and comparison with new design-base computer ...

International Nuclear Information System (INIS)

Experimental data on density, viscosity, and refractive index at T = (298.15, 303.15, and 308.15) K, while speed of sound values at T = 298.15 K are presented for the binary mixtures of (methylcyclohexane + benzene), methylbenzene (toluene), 1,4-dimethylbenzene (p-xylene), 1,3,5-trimethylbenzene (mesitylene), and methoxybenzene (anisole). From these data of density, viscosity, and refractive index, the excess molar volume, the deviations in viscosity, molar refraction, speed of sound, and isentropic compressibility have been calculated. The computed values have been fitted to Redlich-Kister polynomial equation to derive the coefficients and estimate the standard errors. Variations in the calculated excess quantities for these mixtures have been studied in terms of molecular interactions between the component liquids and the effects of methyl and methoxy group substitution on benzene ring.

International Nuclear Information System (INIS)

Formation kinetics and composition of products of interaction of binary and ternary tungsten-molybdenum-rhenium alloys containing 30#+-#3 at.% (27-47 weight %) Re with products of thermal dissociation of ammonia and nitrogen-oxygeneous mixtures are studied. Mo-45% Re-10%W ternary alloy occupies intermediate position between W-27%Re and Mo-47%Re binary alloys, while its corrosion resistance and corrosion nature are close to those of W-Re and mechanical features - to those of Mo-Re alloy. It is explained by the fact, that the variation of W and Mo ration occurs in thin surface layer of ternary alloys in contrast to the initial state and the enrichment of near-the-surface layer with tungsten occurs under hot and cold deformation, at aging in the air, and during oxidation-reduction treatment. 4 refs.; 7 figs.

Energy Technology Data Exchange (ETDEWEB)

The task of designing high performance X-ray optical systemsrequires the development of sophisticated X-ray scattering calculationsbased on rigorous information about the optics. One of the mostinsightful approaches to these calculations is based on the powerspectral density (PSD) distribution of the surface height. The majorproblem of measurement of a PSD distribution with an interferometricand/or atomic force microscope arises due to the unknown ModulationTransfer Function (MTF) of the instruments. The MTF characterizes theperturbation of the PSD distribution at higher spatial frequencies. Here,we describe a new method and dedicated test surfaces for calibration ofthe MTF of a microscope. The method is based on use of a speciallydesigned Binary Pseudo-random (BPR) grating. Comparison of atheoretically calculated PSD spectrum of a BPR grating with a spectrummeasured with the grating provides the desired calibration of theinstrumental MTF. The theoretical ...

International Nuclear Information System (INIS)

Experimental data on A-15 binary phases (the lattice parameter dependence on composition, phase diagrams, etc.) have been considered and the so-called ''compounds with A-15 structure'' are shown to be solid solutions of element substitution in the structure of A-15 type. Values of lattice parameter and atomic volume of A-15 modifications for 21 elements have been obtained (for Zr, W, Nb, Mo, V in particular). There has been shown the possibility of calculation of atomic volumes and lattice parameters of A-15 binary phases at the lack of direct experimental data. The essential role of atomic volume relations of different phases has been observed for the estimation of stability conditions of A-15 phase.

Science.gov (United States)

... tests, one often encounters, for instance, separated flows with large ... It is suspected that the flow-quality criteria given in AGARD Report No. ...

UK PubMed Central (United Kingdom)

Amphibians are considered reliable indicators of environmental quality. In the western United States, a general decline of frog populations parallels an apparent worldwide decline. The factors thought...Full Text Available

International Nuclear Information System (INIS)

The following aspects will be discussed: The main function of the Division of Inspection Services and its role in the Marketing of agricultural products; the shelf life of agricultural products: short review of present methods with a practical example of losses incurred due to limited keeping quality; irradiation and heat treatment: advantages of inhibited microbiological activities and undesirable chemical changes from a quality control point of view; quality standards: the basic principles of quality control; consequences of effective post-harvest treatment: export of deciduous and citrus fruit; the magnitude of the problem of poor keeping quality and quality requirements; other fruit; quantities and export limitations and vegetables; quantity and quality requirements; local marketing: fruit and vegetables subjected to inspection; quantity ...

UK PubMed Central (United Kingdom)

Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients with WAS exhibit both immunodeficiency and a marked susceptibility to systemic autoimmunity. We investigated...Full Text Available

UK PubMed Central (United Kingdom)

Spin-lattice relaxation rates of protein and water protons in dry and hydrated immobilized bovine serum albumin were measured in the range of ¹H Larmor frequency from 10 kHz to 30...Full Text Available

UK PubMed Central (United Kingdom)

Synthetic oligoribonucleotides have been used to probe the interaction of MS2 coat protein with the translational operator of the MS2 replicase gene. We have investigated the possible formation of a...Full Text Available

UK PubMed Central (United Kingdom)

In proteomics, one-dimensional (1D) SDS-PAGE is widely used for protein fractionation prior to mass spectrometric analysis to enhance dynamic range of analysis and to improve identification...Full Text Available

UK PubMed Central (United Kingdom)

We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter...Full Text Available

UK PubMed Central (United Kingdom)

The realization that there existed a G-protein coupled signal transduction mechanism developed gradually and was initially the result of an ill fated quest for uncovering the mechanism of action...Full Text Available

UK PubMed Central (United Kingdom)

The objective of this research was to determine the role of acidic ribosomal protein (ARP) phosphorylation in translation. Ribosomes (Rbs) from germinated maize (Zea mays L.) axes had...Full Text Available

UK PubMed Central (United Kingdom)

Cytosol proteins prepared from castor bean endosperm (4-day-old) seedlings stimulate the exchange of [³H]phosphatidylethanolamine between liposomes and mitochondria. The acceleration of the...Full Text Available

UK PubMed Central (United Kingdom)

Excessive weight loss due to protein calorie malnutrition (PCM) is a significant problem in Nigerian children. This syndrome may be difficult to differentiate from the wasting disease caused by human...Full Text Available

UK PubMed Central (United Kingdom)

Lupus anticoagulant, concentrations of anticardiolipin antibodies, antithrombin III, plasminogen, (free) protein S, protein C, prothrombin, platelet counts, and bleeding times were determined in 74...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundSeveral proteins are known to be markedly expressed in the brain during cerebral ischemia; however, the changes in protein profiles within the ischemic brain after an ischemic...Full Text Available

UK PubMed Central (United Kingdom)

The plasma protein binding of phenytoin was investigated in 100 epileptic patients, using equilibrium dialysis at 37 degrees C. The unbound fractions of phenytoin in plasma formed a skewed distribution,...Full Text Available

UK PubMed Central (United Kingdom)

The purpose of the present work was to study the pharmacokinetics and the protein binding (free fraction of the drug) of ceftriaxone (CTX) during pregnancy. Nine pregnant women (ages, 20 to 34 years)...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundZhx1 to 3 (zinc-fingers and homeoboxes) form a set of paralogous genes encoding multi-domain proteins. ZHX proteins consist of two zinc fingers followed...Full Text Available

UK PubMed Central (United Kingdom)

Genes of the S100 fused-type protein (SFTP) family are clustered within the epidermal differentiation complex and encode essential components that maintain epithelial homeostasis and barrier functions....Full Text Available

UK PubMed Central (United Kingdom)

The exposure of cells to several metal ions stabilizes HIF-1α protein. However, the molecular mechanisms are not completely understood. They may involve inhibition of hydroxylation by either...Full Text Available

UK PubMed Central (United Kingdom)

In this paper, we report a method of precise in situ x-ray scattering measurements on protein solutions using small stationary sample cells. Although reduction in the radiation damage...Full Text Available

UK PubMed Central (United Kingdom)

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

UK PubMed Central (United Kingdom)

Acylation stimulating protein (ASP, C3adesArg) is an adipose tissue derived hormone that stimulates triglyceride (TG) synthesis. ASP stimulates lipoprotein lipase (LPL) activity by relieving feedback...Full Text Available

UK PubMed Central (United Kingdom)

Mammals possess multiple insulin-like growth factor (IGF) binding proteins (IGFBPs), and related proteins, that modulate the activity of insulin/IGF signalling (IIS), a conserved neuroendocrine signalling...Full Text Available

UK PubMed Central (United Kingdom)

Intrinsically disordered proteins are predicted to be highly abundant and play broad biological roles in eukaryotic cells. In particular, by virtue of their structural malleability and propensity...Full Text Available

UK PubMed Central (United Kingdom)

Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G₂...Full Text Available

UK PubMed Central (United Kingdom)

We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO)....Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of...Full Text Available

UK PubMed Central (United Kingdom)

The lysosomal catabolism of sulfatide requires arylsulfatase A and a specific sphingolipid activator protein, SAP-1. While most patients with metachromatic leukodystrophy have mutations in the gene...Full Text Available

UK PubMed Central (United Kingdom)

SummaryMycobacterium tuberculosis has 10 universal stress proteins, whose function is unknown. However, proteomic and transcriptomic analyses have shown that a number...Full Text Available

UK PubMed Central (United Kingdom)

The Moloney murine leukemia virus (MMLV) belongs to the Retroviridae family of enveloped viruses, which is known to acquire minute amounts of host cellular proteins both on the surface...Full Text Available

UK PubMed Central (United Kingdom)

Membrane cofactor protein (MCP or gp45-70) of the complement system is a cofactor for factor I-mediated cleavage of fluid-phase C3b and C3b-like C3, which opens the thioester bond. In the present study...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis ...

UK PubMed Central (United Kingdom)

Mitogen Activated Protein Kinases (MAPKs) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and...Full Text Available

UK PubMed Central (United Kingdom)

A commercial blend of essential oil (EO) compounds was added to a grass, maize silage, and concentrate diet fed to dairy cattle in order to determine their influence on protein metabolism by ruminal...Full Text Available

UK PubMed Central (United Kingdom)

The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater...Full Text Available

UK PubMed Central (United Kingdom)

1 The plasma protein binding of theophylline was determined after addition of [14C]-theophylline (15 micrograms/ml) to plasma from 24 healthy drug-free volunteers and equilibrium dialysis for 2 h at...Full Text Available

UK PubMed Central (United Kingdom)

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...Full Text Available

UK PubMed Central (United Kingdom)

Reversible protein glutathionylation plays a key role in cellular regulation and cell signaling and protects protein thiols from hyperoxidation. Sulfiredoxin (Srx), an enzyme that catalyzes the reduction...Full Text Available

UK PubMed Central (United Kingdom)

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

UK PubMed Central (United Kingdom)

AbstractX-ray diffraction analysis of pressure-induced structural changes in the Aequorea yellow fluorescent protein Citrine reveals the structural basis for the continuous...Full Text Available

UK PubMed Central (United Kingdom)

AbstractThe present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundOne virulence property of Borrelia burgdorferi is its resistance to innate immunity, in particular to complement-mediated killing. Serum-resistant B....Full Text Available

UK PubMed Central (United Kingdom)

Cellular cholesterol homeostasis is controlled by sterol-regulated proteolysis of membrane-bound transcription factors called sterol-regulatory element binding proteins (SREBPs). CPP32, a cysteine protease,...Full Text Available

UK PubMed Central (United Kingdom)

The aim of the present study was to describe the physicochemical characteristics of streptococcal T antigen. T protein isolated from Streptococcus pyogenes type 12 (R53/1077, Colindale) and purified...Full Text Available

UK PubMed Central (United Kingdom)

Recently, CID755673 was reported to act as a highly selective inhibitor of protein kinase D (PKD). In the course of experiments using CID755673, we noticed that it exerted unexpected stimulatory...Full Text Available

UK PubMed Central (United Kingdom)

Sustained nigrostriatal dopamine depletion increases the serine/threonine phosphorylation of multiple striatal proteins that play a role in corticostriatal synaptic plasticity, including Thr²⁸⁶...Full Text Available

UK PubMed Central (United Kingdom)

PurposeCataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and...Full Text Available

UK PubMed Central (United Kingdom)

Amyloid proteins constitute a chemically heterogeneous group of proteins, which share some biophysical and biological characteristics, the principal of which are the high propensity to acquire an incorrect...Full Text Available

UK PubMed Central (United Kingdom)

Albumin is a multifunctional transport protein that binds a wide variety of endogenous substances and drugs. Insulins with affinity for albumin were engineered by acylation of the epsilon-amino group...Full Text Available

UK PubMed Central (United Kingdom)

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to form strong and durable bonds in aqueous environments. These mussel adhesive proteins contain an unusual...Full Text Available

UK PubMed Central (United Kingdom)

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism; its activity is regulated by a plethora of physiological conditions, exercises and many anti-diabetic drugs. Recent...Full Text Available

UK PubMed Central (United Kingdom)

Amyloid precursor protein (APP), implicated in Alzheimer's disease, is a transmembrane protein of undetermined function. APP is cleaved by gamma-secretase that releases the APP intracellular domain...Full Text Available

UK PubMed Central (United Kingdom)

The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundA relevant problem in drug design is the comparison and recognition of protein binding sites. Binding sites recognition is generally based on geometry often combined with...Full Text Available

UK PubMed Central (United Kingdom)

We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this...Full Text Available

UK PubMed Central (United Kingdom)

MOM22 is a component of the protein import complex of the mitochondrial outer membrane of Neurospora crassa. Using the newly developed procedure of 'sheltered disruption', we created a heterokaryotic...Full Text Available

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Science.gov (United States)

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California,

Indian Institute of Science Theses & Dissertations (India)

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International Science & Technology Center (ISTC)

Studies of the Superheavy-metal Effect as a Stress-factor on Protein Biosynthesis and Cytoskeleton Integrity in Animal Cells

International Nuclear Information System (INIS)

Experimental investigation data are systematized of free radical states and processes in irradiated proteins. The investigation is performed by the radiospectral methods. Results are discussed in detail of the study of free radicals electronic structure of amino acids, peptides and proteins formed by the action of ionizing radiation. The specificity is stressed of the study of monocrystalls of these compounds by the method of electronic paramagnetic resonance. The nature is also studied of primary centres formed under the effect of radiation on biologically important compounds and their subsequent reactions in solid and liquid solutions. Ion-radical states of different functional groups of the protein molecule are studied. Prospects of the study and the role of anion-radicals in biological processes are discusses.

KoreaScience (Korea)

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