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Sample records for okra seed abelmoschus

  1. Nutritional evaluation of nigerian dried okra (abelmoschus esculentus) seeds

    The proximate, functional properties, in-vitro multi enzyme protein digestibility and amino acid compositions of nigerian dried okra (abelmoschus esculentus) seeds were evaluated. the ash, moisture, fat, crude fibre, crude protein and carbohydrate of the okra seeds were: 4.8%, 13.5%, 39.9%, 8.82%, 26.4% and 6.62%, respectively. the water and oil absorption capacities were: 220% and 200% which makes okra seeds exhibit a high water retention capacity. the least gelation concentration was 8% while, emulsion capacity was 45.5% and foaming capacity and stability were: 12% and 2%, respectively. the in vitro protein digestibility was 69.3%. glutamic acid was the most concentrated amino acid (121.1 mg/g crude protein) while, cystine (10.0 mg/g crude protein) was the least concentrated amino acid. the total amino acid was 706 mg/g crude protein. (author)

  2. Seed oil and Fatty acid content in okra (Abelmoschus esculentus) and related species

    Approximately 1100 genebank accessions of okra (Abelmoschus esculentus) and 540 additional accessions that included six of its related species were evaluated for seed oil content using TD-NMR. Species evaluated included; A. caillei, A. crinitis, A. esculentus, A. ficulneus, A. manihot, A. moschat...

  3. First identification of ?-glucosidase inhibitors from okra (Abelmoschus esculentus) seeds.

    Thanakosai, Wannisa; Phuwapraisirisan, Preecha

    2013-08-01

    Infusion of roasted okra seeds has long been consumed in Turkey for diabetes mellitus therapy. Previous reports of a hypoglycemic effect observed in rats administrated with okra seed extract indicated a possible connection with inhibition of intestinal alpha-glucosidase. An attempt to identify active components was first herein conducted using alpha-glucosidase-inhibition-guided isolation, yielding two major flavonol glucosides named isoquercetin (2) and quercetin-3-O-beta-glucopyranosyl-(1"' --> 6")-glucoside (3). They selectively inhibited rat intestinal maltase and sucrase, in which isoquercetin (2) was 6-10 times more potent than its related diglucoside 3. This result suggested that an increase in hydrophilicity by the additional glucose residue in 3 led to a significant decline in the inhibitory effect and raised the possible involvement of the free 3-OH in exerting the inhibition. Our postulation was evaluated by examining alpha-glucosidase inhibition of quercetin (1), and the aglycone of 2 and 3, whose 3-OH is free from any glucose moiety. Interestingly, 1 displayed a broad inhibitory effect toward rat intestinal and baker's yeast alpha-glucosidases, with improved potency. A kinetic study of 1 indicated that it inhibited maltase by two distinct mechanisms, in competitive (K(i) 462 microM) and noncompetitive (K(i) 2153 microM) manners, whereas the mechanism underlying the inhibition of sucrase was verified as being of a competitive behavior (K(i) 218 microM). PMID:24079173

  4. Seed-borne mycoflora associated with okra (abelmoschus esculentus (l.) moench)

    Around 75 species belonging to 31 fungal genera were isolated from the eighteen seed samples collected from thirteen localities of Pakistan. Seed-borne mycoflora associated with the samples were isolated and identified by using ISTA techniques. Agar plate method was found best for the isolation of fungi followed by standard blotter method. Seed samples from the areas of Fatu-chuk, Islamabad, Akora Khattak, Mandibahauddin, and Karachi, respectively were found to be highly infected with fungi. Species of Aspergillus and Chaetomium were the most dominant fungi. Species of Fusarium, Phoma, and Macrophomina phaseolina were isolated through both agar plate and standard blotter methods. Variation in size of sclerotia of M. phaseolina was observed. Surface sterilization of seeds with 1% Ca(OCl)2 has reduced the incidence of storage fungi. 32 species belonging to 21 fungal genera are newly reported from Pakistan. (author)

  5. Germination and Seedling Emergence of Primed Okra (Abelmoschus esculentus L. Seeds under Salt Stress and Low Temperature

    Besma Ben Dkhil

    2014-01-01

    Full Text Available This experiment was conducted to evaluate the effects of priming on the germination, emergence and seedling growth of Abelmoschus esculentus (cultivar Marsaouia under low temperature and salinity conditions. Seeds were primed for 24 h at 20°C in three priming media (KCl 4%, mannitol 0.75M, CaCl2 10 mM and control (non-primed seeds and were examined at different salinity levels (0, 40 and 100 mM NaCl. Results indicated that KCl priming increased final germination percentage, radicle length and seedlings dry weight 100%, 40.94 mm and 0.03 g, respectively, as compared with non-primed seeds. Mannitol and CaCl2 have been found to be better treatments for improving final emergence percentage. Overall increased NaCl level, led to the reductions in final germination and emergence percentage but these reductions were higher for non-primed compared to primed seeds. The increase in NaCl concentrations didn’t show any significantly effect on cotyledons fresh weight of primed or non-primed seeds. Besides, our results proved that priming alleviated the adverse effects of salinity for seedlings biomass as compared to non-primed seeds.

  6. EFFECT OF THE ADDITION OF DEFATTED OKRA SEED (Abelmoschus esculentus FLOUR ON THE CHEMICAL COMPOSITION, FUNCTIONAL PROPERTIES AND Zn BIOAVAILABILITY OF PLANTAIN (Musa paradisiacal Linn FLOUR

    Tesleem Ibrahim

    2012-08-01

    Full Text Available In Nigeria it is advised that nursing mothers should give their baby plantain flour paste “amala ogede” with ‘ewedu’ Corchorus olitorius soup during the process of weaning their baby. The over matured okra that cannot be cut with kitchen knife are thrown away in Nigeria, this lead to postharvest loss of okra. The seed in this okra could be utilised by processing into okra seed flour for the fortification of plantain flour. Since the okra seed flour is rich in oil and the oil contains cyclopropenoid fatty acids which cause some toxicity concerns therefore this work is to evaluate the chemical composition, the functional properties and Zn bioavailability of plantain flour mixed with defatted okra seed flour. The nutrient content increased significantly (P≤0.05, protein; 3.88 – 11.38 %, fibre; 3.03 – 16.30 % and ash; 2.72 – 5.77 % while the fat and carbohydrate content reduced significantly (P≤0.05 as the percentage of defatted okra seed flour increased. The bulk density of the plantain flour decreased significantly (P≤0.05 from 0.795 g/cm3 to 0.769 g/cm3 as the percentage of okra seed flour increased while the least gelation concentration increased significantly (P≤0.05 (10 to 20. The calculated [Ca][Phytate]/[Zn] molar ratio for the plantain flour mixed with defatted okra seed flour (0.02 – 0.04 mol/kg were below the critical level. The increase in the least gelation concentration coupled with increase in the protein content of the resultant flour from the blend means more of the protein will be available in the food made from the plantain flour mixed with defatted okra seed flour.

  7. Germination and seedling growth of Okra (Abelmoschus esculentus L.) as influenced by organic amendments

    Banashree Sarma; Nirmali Gogoi

    2015-01-01

    This study was designed to understand the effects of different soil organic amendments on germination and seedling vigour of Okra (Abelmoschus esculentus L.). Five treatments with organic amendments (farmyard manure, vermicompost and biochar) and mineral fertilizers were designed in randomized block design with three replications. Results showed that organic amendments significantly enhanced per cent seed germination and emergence speed index compared to inorganic fertilizer. Highest homogene...

  8. Germination and seedling growth of Okra (Abelmoschus esculentus L. as influenced by organic amendments

    Banashree Sarma

    2015-12-01

    Full Text Available This study was designed to understand the effects of different soil organic amendments on germination and seedling vigour of Okra (Abelmoschus esculentus L.. Five treatments with organic amendments (farmyard manure, vermicompost and biochar and mineral fertilizers were designed in randomized block design with three replications. Results showed that organic amendments significantly enhanced per cent seed germination and emergence speed index compared to inorganic fertilizer. Highest homogeneity of seed germination (CVgt = 20.74 was observed in vermicompost. Plant height, root length and leaf area were higher in vermicompost and biochar than farmyard manure. Both allocation of biomass to above ground parts and Dickson quality index were highest in seedlings from the plots amended with vermicompost. The study revealed that compared to biochar, vermicompost and farmyard manure significantly enhanced the germination and growth of Okra seedling, but the stimulation was best in vermicompost-amended plots.

  9. Salt stress effects on some morphological and physiological characteristics of okra (Abelmoschus esculentus L.

    Muhammad Adnan Shahid*, Muhammad Aslam Pervez, Rashid Mukhtar Balal, Rashid Ahmad, Chaudhary Muhammad Ayyub, Tahira Abbas and Naheed Akhtar

    2011-04-01

    Full Text Available Salinity is worldwide problem that limits the growth and productivity of all vegetations and it is going toincreasing day by day. The objective of the investigation was to study the response of okra (Abelmoschus esculentusL. in terms of morphological and physiological characteristics under salt stress conditions. Okra seeds of cultivarChinese Red were grown in plastic pots having fine sand as growth medium. After 30 days of germination, theplants were subjected to salt stress under control, 25, 50, and 75 mM NaCl. Increasing salinity caused a decrease ofgermination percentage, shoot and root length, plant height, pod weight, pod length, photosynthesis rate, andstomatal conductance. The Na+ and Cl- accumulation were correlated with a decline in concentration of K+ inleaves and roots. Under salinity, a high concentrations of Na+ and Cl- were noted in both leave and roots portions.

  10. Comparative study of quality changes in okra abelmoschus esculentus (L) moench stored at different relative humidities

    Okra (Abelmoschus esculentus L) pods were stored at the relative humidity of 90% and 100% for up to 10 days. The moisture content, crude fibre, and protein, fat, viscosity, hydrolysable and condensed tannin, total phenol, vitamin C and reducing power of the okra were determined on alternate days. Okra pods stored at 100% RH experienced the least percentage loss in all the determined parameters though the loss of antinutrient was lower at this relative humidity. (author)

  11. Effect of polyamines on biochemical properties in gamma irradiated Okra (Abelmoschus esculentus L.) varieties

    Gamma rays are electromagnetic radiations and can be useful for the alteration of physiological characters. In plant systems, gamma ray induced free radicals can damage or modify important components of plant cells and also modulate the morphological, anatomical and biochemical characters of the cell and the plant therein. Polyamine possesses protective role against unfavorable conditions because of its highest positive charge due to presence of amine groups. In plants polyamines are involved in organ development, flowering, fruit ripening, and senescence and stress responses. Okra (Abelmoschus esculentus L.) has captured a prominent position among vegetables and in India; it is grown on an area of 3.6 lakh hectares with a production of 34.2 lakh tones. Keeping these facts in mind, an experiment was conducted to study the effect of polyamines on biochemical properties in gamma irradiated Okra (Abelmoschus esculentus L.) varieties. Seeds of four Okra varieties (namely Kaveri 49, Kaveri 54, Deepika and OH2324) were expose to different levels of gamma radiation (T1 = 200 Gy, T2 = 400 Gy, T3 = 600 Gy, and T4 = 800 Gy) using 60Co as source at National Botanical Research Institute (NBRI) research institute of CSIR at Lucknow. These plants were also subjected to an exogenous application of 1 mM spermine and 1 mM spermidineas foliar spray. The results showed that application of Polyamines (spermine and spermidine) increased Chlorophyll α content, Chlorophyll b content (mg/g fresh weight) in gamma treated plants as compared to control. Application of polyamines also increased enzymatic and non-enzymatic antioxidants (proline, ascorbte peroxidase and glutathione reductase) level in gamma treated plants as compared to control. Carotenoid and protein content showed variations under polyamine treatment. In general, variety Deepika was relatively tolerant to gamma radiation among all the varieties, whereas, Kaveri 49 and Kaveri 54 were relatively sensitive to gamma radiation and the all the plants preformed optimally under 200 Gy gamma exposure. (author)

  12. Physicochemical properties and adsorption of cholesterol by okra (Abelmoschus esculentus) powder.

    Chen, Yi; Zhang, Bing-Cheng; Sun, Yu-Han; Zhang, Jian-Guo; Sun, Han-Ju; Wei, Zhao-Jun

    2015-12-01

    Okra (Abelmoschus esculentus) is a widely used medicine and functional food. In order to clarify the effects of the particle size on its functional properties, okra pods were subjected to superfine grinding, and its properties were determined using different methods. Four particle size levels of okra powders were prepared: 380 to 250, 250 to 75, 75 to 40 and less than 40 μm. The results showed that superfine grinding technology could efficiently pulverize the particles into the submicron scale, whose distribution was close to a Gaussian distribution. With decreasing okra powder size, the specific surface area, water holding capacity (WHC), water-retention capacity (WRC), oil-binding capacity (OBC), tapped density and total flavonoids extraction were increased significantly (p grinding. These results suggest that okra powder can be used in food manufacturing as a functional food ingredient. PMID:26359588

  13. Effect of Salt Stress on Growth, Anthocyanins, Membrane Permeability and Chlorophyll Fluorescence of Okra (Abelmoschus esculentus L. Seedlings

    Mounir Denden

    2012-01-01

    Full Text Available To study the response of okra (Abelmoschus esculentus cv. Marsaouia to salinity, the effect on seedling growth, water content, anthocyanins content and chlorophyll fluorescence was investigated. Okra seeds were germinated in Petri dishes and sown in peat culture under controlled conditions and submitted during 2 weeks to saline stress ranging from 0 (distilled water to 20, 40, 60, 80 and 100 mmol of NaCl. Results showed that salt has no significant effect on aerial part dry weight and it showed no changes in chlorophyll fluorescence parameters (Fo and Fv/Fm. By contrast, root dry weight decreased with increasing NaCl concentration with a subsequent increase in root water content and cotyledons+hypocotyls/root ratio. In addition, anthocyanins content and electrolyte leakage were highly increased by exposure to high levels of salt especially 80 and 100 mmol NaCl.

  14. Effects of morphactin and gamma irradiation on the growth and yield in okra (Abelmoschus esculentus (L. ) Moench. )

    Paliwal, N.K.; Agarwal, S.; Singh, V.P. (Hindu Coll., Moradabad (India))

    1983-06-01

    Investigations were carried out to examine the individual and combined influence of a morphactin (chlorflurenol) (10-100 ppm) and gamma-irradiation(40-60 KR) on growth and yield attributes in okra (Abelmoschus esculentus (L.) Moench) Cv-Pusa Sawani. Morphactin caused inhibition of vegetative growth and weakened apical dominance. Gamma-irradiation of seeds reduced the growth and stimulated the branching. The combinations of gamma radiation and morphactin were found to inhibit the growth more vigorously. Morphactin application promoted fruit-set and yield at all the used concentrations. Gamma-irradiation enhanced the fruit-set and yield at 40 and 50 KR and reduced drastically at 60 KR. Combination of gamma radiation (40 KR) with morphactin (10-50 ppm) increased the yield while other combinations reduced this factor in okra.

  15. Effects of morphactin and gamma irradiation on the growth and yield in okra (Abelmoschus esculentus (L.) Moench.)

    Investigations were carried out to examine the individual and combined influence of a morphactin (chlorflurenol) (10-100 ppm) and gamma-irradiation(40-60 KR) on growth and yield attributes in okra (Abelmoschus esculentus (L.) Moench) Cv-Pusa Sawani. Morphactin caused inhibition of vegetative growth and weakened apical dominance. Gamma-irradiation of seeds reduced the growth and stimulated the branching. The combinations of gamma radiation and morphactin were found to inhibit the growth more vigorously. Morphactin application promoted fruit-set and yield at all the used concentrations. Gamma-irradiation enhanced the fruit-set and yield at 40 and 50 KR and reduced drastically at 60 KR. Combination of gamma radiation (40 KR) with morphactin (10-50 ppm) increased the yield while other combinations reduced this factor in okra. (author)

  16. Inheritance of resistance to Okra yellow vein mosaic disease in interspecific crosses of Abelmoschus.

    Jambhale, N D; Nerkar, Y S

    1981-09-01

    Two Abelmoschus species, viz., A. manihot (L.) Medik and A. manihot (L.) Medik ssp. manihot, resistant to Okra yellow vein mosaic (YVM) were crossed to A. esculentus cv. 'Pusa Sawani', a susceptible culture. The hybrids were resistant and partially fertile. Segregation pattern for disease reaction in F2, BC1 and subsequent generations of the two crosses revealed that resistance to YVM is controlled by a single dominant gene in each species. PMID:24276872

  17. Studies on Heterosis in Okra (Abelmoschus esculentus (L.) Moench)

    P.Senthil Kumar and S. Sreeparvathy

    2010-01-01

    In the present investigation, a study was undertaken at the Department of Agricultural Botany, Annamalai University during the year 2006 – 2008to identify potential parents and superior cross combinations for yield improvement in Okra. Six bhendi genotypes viz., Girija Vikas, MDU 1,Hissar Unnath, Arka Abhay and EC 305623 were crossed in full diallel fashion (including the reciprocals). The eight characters observedincluded days to 50 per cent flowering, plant height, number of branches per pl...

  18. Relative effect of organic and inorganic fertilizers on the growth of okra [Abelmoschus esculentus (L.) Moench

    Uka Ufere N.; Chukwuka Kanayo S.; Iwuagwu Mary

    2013-01-01

    A greenhouse experiment was conducted to study the relative effect of organic and inorganic fertilizers on the growth of okra (Abelmoschus esculentus). The experiment consisted of four treatments. Cow dung and poultry droppings were applied at the rates of 2.5 kg per 10 kg of soil one week before sowing, while NPK fertilizer (15:15:15) was applied at the rate of 6 g per 10 kg of soil three weeks after sowing. The treatments were laid in a completely randomi...

  19. Heterosis for Yield and Yield Components in Okra (Abelmoschus esculentus (L.) Moench) Heterosis para Producción y Componentes del Rendimiento en Gombo (Abelmoschus esculentus (L.) Moench)

    Thirupathi Reddy Medagam; Haribabu Kadiyala; Ganesh Mutyala; Begum Hameedunnisa

    2012-01-01

    The study of heterosis would help in selection of heterotic crosses for commercial exploitation of F1 hybrids in okra (Abelmoschus esculentus (L.) Moench). Forty five F1S were developed by crossing 10 elite lines of okra: P1(IC282248), P2(IC27826-A), P3(IC29119-B), P4(IC31398-A), P5(IC45732), P6(IC89819), Py(IC89976), P8(IC90107), P9(IC99716), and P10(IC111443), in half diallel fashion during summer 2009. All 45 F1s along with their 10 parents and one standard control (Mahyco Hybrid N° 10) we...

  20. Studies on Heterosis in Okra (Abelmoschus esculentus (L. Moench

    P.Senthil Kumar and S. Sreeparvathy

    2010-12-01

    Full Text Available In the present investigation, a study was undertaken at the Department of Agricultural Botany, Annamalai University during the year 2006 – 2008to identify potential parents and superior cross combinations for yield improvement in Okra. Six bhendi genotypes viz., Girija Vikas, MDU 1,Hissar Unnath, Arka Abhay and EC 305623 were crossed in full diallel fashion (including the reciprocals. The eight characters observedincluded days to 50 per cent flowering, plant height, number of branches per plant, number of fruits per plant, fruit length, fruit girth, individualfruit weight, fruit yield per plant. The results revealed that the standard heterosis for fruit yield per plant was maximum in the hybrid MDU 1 xHissar Unnath, with a value of 65.23 per cent. This hybrid recorded high standard heterosis for all the characters except number of branches perplant and individual fruit weight.

  1. Effects of soil amendments on the nutritional quality of okra (Abelmoschus esculentus [L.]Moench

    M. B Adewole

    2011-01-01

    Full Text Available This study examined the effects of different soil amendments [compost organic fertiliser (OR, NPK (IO, Glomus mosseae mycorrhiza (MY or no soil amendment as the control (CT] on the nutritional quality and nutrient uptake of okra during cultivation in a field contaminated with sewage sludge from the two oxidation ponds of the Obafemi Awolowo University, Ile-Ife, Nigeria. Okra (Abelmoschus esculentus [L.] Moench belongs to the Malvacea family. The experiment consisted of a randomised complete block design with four replications. At full physiological maturity, the roots, shoots and pods samples of the okra plants were collected for analyses. The results showed that OR resulted in a significantly (p < 0.05 higher nutrient uptake [N (0.0034 mg kg-1, K (0.0160 mg kg-1, Na (0.9753 mg kg-1, Ca (0.0130 mg kg-1 and Cu (0.01136 mg kg-1] in the okra roots than in the other treatments, yet the significantly (p < 0.05 highest uptake of P (0.0012 mg kg-1 was obtained with the MY treatment. Lower values of these nutrient contents were obtained in the shoots. The control treatment gave the significantly highest values of crude fibre (27.33% and total ash (14.05%, as compared to the other treatments, whereas the other nutritional properties obtained showed no significant difference among any of the treatments. The results indicated that high-quality okra pods could be effectively produced with no soil amendment when planted in soils with a high fertility, such as those treated with sewage sludge.

  2. Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers.

    Yuan, C Y; Zhang, C; Wang, P; Hu, S; Chang, H P; Xiao, W J; Lu, X T; Jiang, S B; Ye, J Z; Guo, X H

    2014-01-01

    Okra (Abelmoschus esculentus L.) is not only a nutrient-rich vegetable but also an important medicinal herb. Inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 24 okra genotypes. In this study, the PCR products were separated by electrophoresis on 8% nondenaturing polyacrylamide gel and visualized by silver staining. The 22 ISSR primers produced 289 amplified DNA fragments, and 145 (50%) fragments were polymorphic. The 289 markers were used to construct the dendrogram based on the unweighted pair-group method with arithmetic average (UPGMA) cluster analysis. The dendrogram indicated that 24 okras were clustered into 4 geographically distinct groups. The average polymorphism information content (PIC) was 0.531929, which showed that the majority of primers were informative. The high values of allele frequency, genetic diversity, and heterozygosity showed that primer-sample combinations produced measurable fragments. The mean distances ranged from 0.045455 to 0.454545. The dendrogram indicated that the ISSR markers succeeded in distinguishing most of the 24 varieties in relation to their genetic backgrounds and geographical origins. PMID:24841648

  3. The okra (Abelmoschus esculentus) transcriptome as a source for gene sequence information and molecular markers for diversity analysis.

    Schafleitner, Roland; Kumar, Sanjeet; Lin, Chen-Yu; Hegde, Satish Gajanana; Ebert, Andreas

    2013-03-15

    A combined leaf and pod transcriptome of okra (Abelmoschus esculentus (L.) Moench) has been produced by RNA sequencing and short read assembly. More than 150,000 unigenes were obtained, comprising some 46 million base pairs of sequence information. More than 55% of the unigenes were annotated through sequence comparison with databases. The okra transcriptome sequences were mined for simple sequence repeat (SSR) markers. From 935 non-redundant SSR motifs identified in the unigene set, 199 were chosen for testing in a germplasm set, resulting in 161 polymorphic SSR markers. From this set, 19 markers were selected for a diversity analysis on 65 okra accessions comprising three different species, revealing 58 different genotypes and resulted in clustering of the accessions according to species and geographic origin. The okra gene sequence information and the marker resource are made available to the research community for functional genomics and breeding research. PMID:23299025

  4. Lectin of Abelmoschus esculentus (okra) promotes selective antitumor effects in human breast cancer cells.

    Monte, Leonardo G; Santi-Gadelha, Tatiane; Reis, Larissa B; Braganhol, Elizandra; Prietsch, Rafael F; Dellagostin, Odir A; E Lacerda, Rodrigo Rodrigues; Gadelha, Carlos A A; Conceição, Fabricio R; Pinto, Luciano S

    2014-03-01

    The anti-tumor effects of a newly-discovered lectin, isolated from okra, Abelmoschus esculentus (AEL), were investigated in human breast cancer (MCF7) and skin fibroblast (CCD-1059 sk) cells. AEL induced significant cell growth inhibition (63 %) in MCF7 cells. The expression of pro-apoptotic caspase-3, caspase-9, and p21 genes was increased in MCF7 cells treated with AEL, compared to those treated with controls. In addition, AEL treatment increased the Bax/Bcl-2 ratio in MCF7 cells. Flow cytometry also indicated that cell death (72 %) predominantly occurred through apoptosis. Thus, AEL in its native form promotes selective antitumor effects in human breast cancer cells and may represent a potential therapeutic to combat human breast cancer. PMID:24129958

  5. Characterisation of twenty-nine (29) accessions of okra (Abelmoschus spp (L.) Moench) in Ghana

    A series of investigations were carried out to determine the genetic variability within 29 accessions of okra (Abelmoschus spp (L.) Moench) through characterisation using morphological, biochemical, nutritional and molecular markers. The goal was to obtain information on key traits of okra germplasm relevant to breeders and other researchers towards improvement of the crop. Twenty six (26) indigenous (landraces) and three (3) exotic accessions of okra were collected from eight regions of Ghana and their morpho-agronomic traits were evaluated under field conditions at the Biotechnology and Nuclear Agriculture Research Institute (BNARI) research fields using the International Plant Genetic Research Institute (IPGRI) descriptor list for okra. The 29 exhibited significant variation in all but two quantitative traits studied. Block coefficients of variation were extremely low, implying that results obtained are reliable and repeatable over replications. The 29 accessions were grouped into two major clusters and subsequently into five sub-clusters based on both quantitative and qualitative characters studied. The association between pairs of quantitative yield traits in the okra landraces revealed that flowering and fruiting parameters had significant (P < 0.01) positive associations. Factor scores of 12 characters contributed substantially to total genetic variation among the 29 okra accessions studied. The pattern of clustering did not show distinct association between morpho-agronomic characters and geographic origin of the collections. The output of the Principal Components Analysis (PCA) revealed that different characters contributed differently to total genetic variation. The means of maximum viscosity values for mucilage extracted from the fruits ranged from 53.0 - 366.8bu, with three accessions; DKA (366.8bu), Yeji-Local (329bu) and Amanfrom (316.8bu) recording very high values whilst Cape (53.0bu) had the least maximum viscosity value. There was low level of polymorphism detected among all accessions using inter-simple sequence repeat (ISSR) markers. The accessions, Atomic and Akrave were detected to have originated from a common ancestry. While there was high variability among Okra accessions for the amounts of flavonoids, phenolics and total antioxidant activity in the fresh fruits and the quantities were generally high making okra a good source of natural antioxidants. Ethanol extraction yielded better antioxidant activity than aqueous (water) solvent. The accession, Agric short fruit recorded the highest total flavonoid content (TFC) of 5159.21±12.90μg/g/QE while Cs-Legon had the lowest TFC of 2003.69±2.55μg/g/QE in the ethanol extract. On the other hand, Kortebortor-ASR registered the highest total phenolic content of 63.22 ±3.95μ/g/GAE) while Volta had the lowest TPC of 6.82±0.09μ/g/GAE in the aquous extract. Debo and Kortebortor-ASR recorded the highest (25.83±5.30μ/g/GAE) and lowest (8.0±0.37μ/g/GAE) Total Phenolics Content in the ethanol extract respectively. Nine essential mineral elements (sodium, magnesium, potassium, calcium, bromine, chlorine, copper, aluminium and manganese) were detected among all accessions using Instrumental Neutron Activation Analysis (INAA). There was significant variation in concentrations of these elements found in fresh fruits of the accessions. There were strong positive associations between five pairs of elements contained in the fruits of the accessions of okra.(au)

  6. Genetic architecture of resistance to yellow vein mosaic and leaf spot diseases in okra (Abelmoschus esculentus (L.) Moench)

    P. Sindhumole and P. Manju

    2015-01-01

    Generation mean analysis of four crosses of okra (Abelmoschus esculentus (L.) Moench) viz., NBPGR/TCR-1498 x NBPGR/TCR-2060, NBPGR/TCR-2019 x Parbhani Kranti, MDU-1 x Varsha Uphar, NBPGR/TCR-985 x Parbhani Kranti was conducted for studying gene action of resistance to two major diseases viz., yellow vein mosaic (YVM) and Alternaria leaf spot under Kerala conditions. Duplicate gene action was observed for resistance to both diseases, which indicated hindrance to improvement by simple selection...

  7. Abelmoschus esculentus (L.) Moench. and Abelmoschus moschatus Medik: seeds production and analysis of the volatile compounds.

    Molfetta, Ilaria; Ceccarini, Lucia; Macchia, Mario; Flamini, Guido; Cioni, Pier Luigi

    2013-11-01

    Nine accessions of Abelmoschus esculentus (L.) Moench. and three of Abelmoschus moschatus Medik were both grown in Central Italy for the evaluation of the production of seeds. Furthermore, the volatiles emitted by the mature seeds were sampled by mean of SPME. Seventy compounds were detected in the headspace of the seeds of A. esculentus. The principal constituents common to all the nine accessions were isopentyl 2-methyl butanoate (24.5-59.1%) and heptanoic acid 2-methylbutyl ester (6.6-13.5%). In the headspace around the seeds of A. moschatus 93 components were detected. Among the main volatiles shared by the three accessions, n-tridecane (1.5-26.9%), isopentyl 2-methyl butanoate (0.2-14.3%) and decanal (1.6-5.7%) should be mentioned. Many differences were present in the volatiles emitted by the various accessions and between the two Abelmoschus species. PMID:23768323

  8. Screening of Fe-Deficiency Tolerance in Okra (Abelmoschus esculentus L. Through Hydroponic Culture

    Motiar RAHMAN

    2014-09-01

    Full Text Available Screening for Fe deficiency tolerance in okra (Abelmoschus esculentus Bangladeshi genotypes (‘BARI-1’, ‘Local variety’, ‘Orca Onamica’, and ‘Prince’ were studied based on different morphological and physiological parameters. Number of leaves, shoot height and weight were significantly reduced in ‘Orca Onamica’ and ‘Prince’, whereas ‘BARI-1’ and ‘Local variety’ did not show prominent decrease in the aforesaid growth parameters under Fe deficiency. Again, ‘Orca Onamica’ and ‘Prince’ showed significantly decreased root length and root biomass under Fe deficiency. In contrast, these parameters were unchangeable in ‘BARI’-1 and ‘Local variety’ in Fe shortage compared to controls. Furthermore, Fe deficiency caused severe decrease in chlorophyll (a and b and Fe concentrations in leaves of ‘Orca Onamica’ and ‘Prince’ grown on hydroponic culture. In contrast, chlorophyll (a and b and Fe concentrations were not significantly decreased in ‘BARI-1’ and ‘Local variety’ due to Fe deficiency. Based on these findings, tolerance to Fe deficiency in these okra cultivars can be categorized as: tolerant (‘BARI-1’ and ‘Local’, and sensitive (‘Orca Onamica’ and ‘Prince’. The ranking can be applied in plant breeding program and may have great advantage over conventional methods. This study also demonstrates the effectiveness of hydroponic culture as an efficient method to screen Fe-efficient crop plants.

  9. Micorrización de okra (abelmoschus esculentus l. en riego por goteo

    Manuel Alvarado Carrillo

    2007-01-01

    Full Text Available Se estudió el efecto del hongo micorrízico arbuscular (HMA Glomus intraradices en la productividad de okra (Abelmoschus esculentus L. var. ‘Clemson Spineless’ en condiciones semiáridas y bajo un sistema de riego por goteo. En 2002 y 2003 se comparó la altura de planta (AP, colonización micorrízica (CM y el rendimiento comercial de fruto (RCF, en plantas originadas de semillas, con o sin la inoculación micorrízica. En 2003 el riego fue con agua con alta concentración de sales (conductividad eléctrica de 3000 mS cm-1. En 2004 se evaluó el índice de clorofila (IC, la AP y el RFC, en plantas de okra de los tratamientos: semilla inoculada con el HMA, con fertilización química (120N-50P-00K, con la combinación del HMA en semilla más 50 % de la fertilización química y el testigo. En 2002 y 2003 se registraron incrementos significativos del HMA en CM y RFC, con relación al testigo. Con agua de riego salina en 2003, se observaron mayores valores de CM y RFC cuando se inoculó con G. intraradices. En 2004 los tratamientos no influyeron significativamente en IC ni en AP; no obstante, en RFC los tratamientos HMA, fertilización química y HMA más 50 % de fertilización, superaron al testigo. Los resultados muestran que G. intraradices aumentó el RFC de okra durante los tres años en el sistema de riego por goteo. El efecto benéfico de la simbiosis con el HMA como promotor del rendimiento de fruto de okra, combinado con el sistema de riego por goteo, pueden representar un manejo de producción eficiente para regiones semiáridas.

  10. Genetic analysis for yield and its components in okra (Abelmoschus esculentus (L. Moench

    Konda Chandrasekhar Reddy4

    2012-04-01

    Full Text Available Combining ability variances and effects of yield and its components in okra (Abelmoschus esculentus (L. Moenchwere studied through half diallel analysis of 45 F1 hybrids derived by crossing 10 nearly homozygous germplasm lines namelyP1(IC282248, P2(IC27826-A, P3(IC29119-B, P4(IC31398-A, P5(IC45732, P6(IC89819, P7(IC89976, P8(IC90107, P9(IC99716 andP10(IC111443 during mid kharif (July-October, 2009, at the Vegetable Research Station, Rajendranagar, Andhra Pradesh,India. Both additive and non-additive variances were important for a majority of the characters except plant height, fruit andshoot borer infestation on fruits and shoots and yellow vein mosaic virus infestation on plants. Genetic analysis revealed apreponderance of non-additive gene action for plant height, internodal length, days to 50% flowering, first flowering andfruiting node, fruit length and weight, total number of fruits and number of marketable fruits per plant, total yield and marketableyield per plant and yellow vein mosaic virus infestation on fruits and plants and a preponderance of additive gene actionfor number of branches per plant and fruit and shoot borer infestation on fruits and shoots. The parents P5(IC45732, P6(IC89819 and P7(IC89976 were high general combiners for total and marketable yield per plant and their associated traits,which could be exploited for developing prolific pure line varieties of okra. The crosses C23(IC29119-B × IC99716, C17(IC27826-A × IC111443, C42(IC89976 × IC111443 were superior specific combiners for total and marketable yield per plant withthe potential of being commercially exploited for the production of F1 hybrids. The cross combinations C42 (IC89976 × IC111443 and (IC27826-A × IC89819 having one of the parents with positively significant general combining ability effects fortotal yield and marketable yield per plant could be utilized in recombination breeding with single plant selection in the passinggenerations to capitalize additive gene action to develop high yielding lines or varieties in okra.

  11. Effect of Drip Irrigation Levels and Emitters Depth on Okra (Abelmoschus esculentus) Growth

    AL-HARBI, A. R.; A.M. Al-Omran; F.I. El-Adgham

    2008-01-01

    Okra Abelmoschus esculentus (Climson Spineless CV.) growth, rooting, yield and water use efficiency were evaluated in a field trial, where four irrigation rates at 60 (T1), 80 (T2), 100 (T3) and 120% (T4) of the estimated evapotranspiration (ETo) and four drip irrigation emitters depth: surface 0.0 m depth (D0), sub-surface at 0.15 m depth (D1), at 0.25 m depth (D2) and at 0.35 m depth (D3) were imposed following a split-plot in a randomized complete block experimental design with thre...

  12. Screening of Fe-Deficiency Tolerance in Okra (Abelmoschus esculentus L.) Through Hydroponic Culture

    Motiar RAHMAN; Mohammad S Islam; Kabir, Ahmad Humayan; Haider, Syed A.; Nishit K. PAUL

    2014-01-01

    Screening for Fe deficiency tolerance in okra (Abelmoschus esculentus) Bangladeshi genotypes (‘BARI-1’, ‘Local variety’, ‘Orca Onamica’, and ‘Prince’) were studied based on different morphological and physiological parameters. Number of leaves, shoot height and weight were significantly reduced in ‘Orca Onamica’ and ‘Prince’, whereas ‘BARI-1’ and ‘Local variety’ did not show prominent decrease in the aforesaid growth parameters under Fe deficiency. Again, ‘Orca Onamica’ and ‘Prince’ showed si...

  13. Genetic architecture of resistance to yellow vein mosaic and leaf spot diseases in okra (Abelmoschus esculentus (L. Moench

    P. Sindhumole and P. Manju

    2015-03-01

    Full Text Available Generation mean analysis of four crosses of okra (Abelmoschus esculentus (L. Moench viz., NBPGR/TCR-1498 x NBPGR/TCR-2060, NBPGR/TCR-2019 x Parbhani Kranti, MDU-1 x Varsha Uphar, NBPGR/TCR-985 x Parbhani Kranti was conducted for studying gene action of resistance to two major diseases viz., yellow vein mosaic (YVM and Alternaria leaf spot under Kerala conditions. Duplicate gene action was observed for resistance to both diseases, which indicated hindrance to improvement by simple selection. Hence reciprocal recurrent selection would be useful for the effective utilisation of both types of additive and non-additive gene effects simultaneously.

  14. Phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger (Abelmoschus esculentus L.) in mice.

    Doreddula, Sathish Kumar; Bonam, Srinivasa Reddy; Gaddam, Durga Prasad; Desu, Brahma Srinivasa Rao; Ramarao, Nadendla; Pandy, Vijayapandi

    2014-01-01

    Abelmoschus esculentus L. (ladies finger, okra) is a well-known tropical vegetable, widely planted from Africa to Asia and from South Europe to America. In the present study, we investigated the in vitro antioxidant capacity and in vivo protective effect of the aqueous and methanolic seed extracts of Abelmoschus esculentus against scopolamine-induced cognitive impairment using passive avoidance task and acute restraining stress-induced behavioural and biochemical changes using elevated plus maze (EPM) and forced swimming test (FST) in mice. Our results demonstrated that the pretreatment of mice with aqueous and methanolic seed extracts of Abelmoschus esculentus (200 mg/kg, p.o.) for seven days significantly (P esculentus L. possess antioxidant, antistress, and nootropic activities which promisingly support the medicinal values of ladies finger as a vegetable. PMID:25401145

  15. Growth and Yield of Okra (Abelmoschus esculentus Moench as Influenced by Compost Application under Different Light Intensities

    Victor A. DADA

    2015-06-01

    Full Text Available Okra (Abelmoschus esculentus is one of the popular vegetables, especially rich in iron, vitamins and other minerals. Poor soil fertility and inconsistent light intensity, due to unfavorable weather condition, reduce okra performance. Response of two okra varieties (‘NH47-4’ and ‘Clemson spine’ to different rates of compost (0, 5, 10 and 15 t/ha, under different light intensities (L0: control (no reduction or 100% light intensity, L1: 33%, L2: 46% and L3: 76%, light reduction were assessed in pot and field trials. The experimental design was a factorial experiment fitted in a randomized complete block design (RCBD. Data on growth and yield attributes were collected. The results showed that the reduction in light intensity (L3 increased the numbers of fruits and leaf area by 50 and 47% respectively on the field, but delayed flowering. High light intensity (L0 though enhanced leaf area formation and early flowering, but hastened leaf senescence and abscission. Compost generally increased growth rate, leaf area and dry matter accumulation of the two okra cultivars compared to control under varying light intensities. Compost at 15 t/ha performed better and increased fruit number by 66% on the field. Between the two cultivars, ‘Clemson spine’ responded better than ‘NH47-4’ plants in terms of yield. Low light intensity (76% light reduction in combination with higher compost rate however enhanced prolonged fruiting and leaf formation in the two okra varieties. The application of compost at 15 t/ha is therefore recommended for optimum yield of okra under low light intensity.

  16. Induction of genetic variability in okra [Abelmoschus esculentus (L. Moench] by gamma and ems.

    Peddi Sudharshan Reddy, L. K. Dhaduk

    2014-09-01

    Full Text Available An investigation was carried out to study the extent of genetic variability induced through physical and chemical mutagens in okra. Seeds of two popular okra varieties viz. GO-2 and GJO-3 were treated with 20 kR, 30 kR and 40 kR of gamma rays and 0.15% and 0.25% EMS to raise M1 generation in summer 2011 and the seed obtained from all of the M1 competitive plants were used to raise M2 in progeny rows of each of the treatments including control (the untreated one in kharif 2011.In M2 generation, increase in GCV, heritability and genetic advance was considerable under different mutagenic treatments for most of the traits studied in both varieties. In GO-2, high heritability and genetic advance was observed for some important yield contributing characters like fruit length at 40 kR and 0.25% EMS; fruit weight at 20 kR and 40 kR; and fruit yield per plant at 20, 30, 40 kR and 0.15% EMS.Similarly in GJO-3, high heritability coupled with high genetic advance was observed for fruit length at 30 kR and fruit yield per plant at 20 kR. YVMV percentage exhibited high PCV and GCV with high heritability and genetic advance in 0.25% EMS treatment in GO-2 and 40kR treatment in GJO-3. Thus selection for these characters at specific mutagenic treatments will be effective for improvement in okra.

  17. Effect of Drip Irrigation Levels and Emitters Depth on Okra (Abelmoschus esculentus Growth

    A.R. Al-Harbi

    2008-01-01

    Full Text Available Okra Abelmoschus esculentus (Climson Spineless CV. growth, rooting, yield and water use efficiency were evaluated in a field trial, where four irrigation rates at 60 (T1, 80 (T2, 100 (T3 and 120% (T4 of the estimated evapotranspiration (ETo and four drip irrigation emitters depth: surface 0.0 m depth (D0, sub-surface at 0.15 m depth (D1, at 0.25 m depth (D2 and at 0.35 m depth (D3 were imposed following a split-plot in a randomized complete block experimental design with three replications in 2005 and 2006 seasons. Vegetative growth characters (plant height, number of leaves, shoot fresh and dry weight, rooting (weight, length, width and root/shoot ratio, early and total yields were measured. Marketable Total Yield (MTY increased significantly with the increase of irrigation level in both seasons. MTY for T3 treatments were 14.32 t ha-1 in 2005 and 10.29 t ha-1 in 2006 and for D1 treatments were 10.8 t ha-1 in 2005 and 9.75 t ha-1 in 2006 season. The Crop Water Use Efficiency (CWUE ranged from 1.45-2.93 kg m-3 and 1.29-2.43 kg m-3 in 2005 and 2006, respectively. MTY increased significantly as emitter depth increases from surface to 0.35 m in both seasons. Crop Water Production Function (CWPF was done on the results. It reflects the beneficial of applied water in increasing yield. The CWPF was represented by quadratic polynomial equations.

  18. Optimisation of extraction and sludge dewatering efficiencies of bio-flocculants extracted from Abelmoschus esculentus (okra).

    Lee, Chai Siah; Chong, Mei Fong; Robinson, John; Binner, Eleanor

    2015-07-01

    The production of natural biopolymers as flocculants for water treatment is highly desirable due to their inherent low toxicity and low environmental footprint. In this study, bio-flocculants were extracted from Hibiscus/Abelmoschus esculentus (okra) by using a water extraction method, and the extract yield and its performance in sludge dewatering were evaluated. Single factor experimental design was employed to obtain the optimum conditions for extraction temperature (25-90 °C), time (0.25-5 h), solvent loading (0.5-5 w/w) and agitation speed (0-225 rpm). Results showed that extraction yield was affected non-linearly by all experimental variables, whilst the sludge dewatering ability was only influenced by the temperature of the extraction process. The optimum extraction conditions were obtained at 70 °C, 2 h, solvent loading of 2.5 w/w and agitation at 200 rpm. Under the optimal conditions, the extract yield was 2.38%, which is comparable to the extraction of other polysaccharides (0.69-3.66%). The bio-flocculants displayed >98% removal of suspended solids and 68% water recovery during sludge dewatering, and were shown to be comparable with commercial polyacrylamide flocculants. This work shows that bio-flocculants could offer a feasible alternative to synthetic flocculants for water treatment and sludge dewatering applications, and can be extracted using only water as a solvent, minimising the environmental footprint of the extraction process. PMID:25929197

  19. Correlation and path coefficient analysis of quantitative characters in okra (Abelmoschus esculentus (L. Moench

    Reddivenkatagari Subbarama Krishna Reddy

    2013-06-01

    Full Text Available One hundred germplasm lines of okra (Abelmoschus esculentus (L. Moench were evaluated in a randomized block design with two replications at the Vegetable Research Station, Rajendranagar, Hyderabad, Andhra Pradesh, India, during kharif, 2008. Correlation and path coefficient analysis were carried out to study the character association and contribution, respectively, for thirteen quantitative characters, namely plant height (cm, number of branches per plant, internodal length(cm, days to 50% flowering, first flowering node, first fruiting node, fruit length (cm, fruit width (cm, fruit weight (g, total number of fruits per plant, number of marketable fruits per plant, total yield per plant (g and marketable yield per plant (g for the identification of appropriate selection indices. Phenotypic and genotypic correlation coefficient analysis revealed that plant height, fruit length, fruit width, fruit weight, total number of fruits per plant, number of marketable fruits per plant and total yield per plant had significant positive correlation, while number of branches per plant, internodal length, days to 50% flowering, first flowering node and first fruiting node had significant negative correlation with marketable yield per plant.Genotypic path coefficient analysis revealed that fruit weight, total number of fruits per plant and number of marketable fruits per plant had positively high direct effect on marketable pod yield per plant. Correlation and path coefficient analyses revealed that fruit weight, total number of fruits per plant and number of marketable fruits per plant not only had positively significant association with marketable pod yield per plant, but also had positively high direct effect on marketable pod yield per plant and are regarded as the main determinants of marketable pod yield per plant. The improvement in marketable pod yield per plant will be efficient, if the selection is based on fruit weight, total number of fruits per plant and number of marketable fruits per plant.

  20. 'Anjitha'- A new okra variety through induced mutation in inter specific hybrids of Abelmoschus SPP

    Studies on inter specific hybrids of okra between A. esculentus (cultivated type) and A. manihot (wild type) revealed that no useful recombinants were obtained from the conventional combination breeding programme because of the strong linkage between yellow vein mosaic (YVM) resistant genes and wild character of A. manihot. The present study envisaged the breaking of undesirable linkage through gamma irradiation (10, 20, 30 and 40 kRad) of F1 seeds obtained by inter specific hybridization between A. esculentus var. Kiran and A. manihot and further evaluation and selection of high yielding YVM resistant types from the segregating generations till F6M6. The mutagenic effectiveness and efficiency increased with increasing doses of gamma rays. The mutated hybrids and the wild parent showed complete resistance to YVM disease incidence which was confirmed through grafting trials. In the segregating generations, the irradiated treatments were late flowering and had more number of leaves, flowers and fruits per plant. Average fruit weight was maximum in 20 kR while fruit yield was maximum in 40 kR due to larger number of fruits. A few high yielding disease resistant plants resembling the cultivated plants were obtained in 30kR which suggested that 30 kR could be the ideal irradiation dose in okra. Thirteen superior genotypes selected from F6M6 generation based on yield and YVM resistance were advanced to three Comparative Yield Trials (CYT). Culture AE18 out yielded the others in CYTs and Farm trials and was released as 'Anjitha' by the XXIII State Seed Sub Committee during 2006 for cultivation in Thiruvananthapuram District of Kerala. Anjitha is a high yielding variety having the fruit characters and quality of the cultivated parent A. esculentus var. Kiran combined with the YVM resistant character of the wild parent A. manihot. (author)

  1. Nature and magnitude of genetic variability and diversity studies in okra (Abelmoschus esculentus L. Moench

    K. Prakash and M. Pitchaimuthu

    2010-12-01

    Full Text Available In the present investigation, an attempt has been made to evaluate the genetic variability of yield contributing characters, and the genetic diversityin forty-four genotypes of okra collected from the IIHR, Bangalore, India. Analysis of variance indicated significant differences among thegenotypes for different morphological characters. High GCV and PCV were observed for plant height, inter-nodal length, first flowering node,first fruit producing node, height of first flowering node, average fruit weight and number of seeds per fruit. On the basis of D2 analysis, the 44genotypes were grouped into twelve clusters. The cluster III was the largest with eight genotypes followed by cluster I and VIII with seven,cluster II with five, cluster XII with three while, clusters IV, V, VI, IX, X and XI included only two genotype in each. The intra-cluster distancewas maximum in cluster XII (28.14, while inter-cluster distance was maximum between cluster VI and VIII (35.57 followed by I and IX(35.31, thus being a good source for attempting hybridization. Among the 44 genotypes, IIHR-238, IIHR-241 showed maximum number offruits per plant and total yield per plant (g. The characters namely days to 50% flowering (35.62%, 100 seed weight (28.44%, number of seedsper fruit (17.23% and average fruit weight (8.14% directly contributed towards maximum divergence and, therefore, selection of divergentparents based on these characters is recommended for getting good hybrids or segregants in okra.

  2. Evaluation of fatty acid and amino acid compositions in okra (Abelmoschus esculentus) grown in different geographical locations.

    Sami, Rokayya; Lianzhou, Jiang; Yang, Li; Ma, Ying; Jing, Jing

    2013-01-01

    Okra has different uses as a food and a remedy in traditional medicine. Since it produces many seeds, distribution of the plant is also quite easy. Although seed oil yield is low (4.7%), since the linoleic acid composition of the seed oil is quiet high (67.5%), it can still be used as a source of (UNSAT) unsaturated fatty acids. In this study, samples of okra grown in four different locations were analyzed to measure fatty acid and amino acid compositions. The content of the lipid extraction ranged from 4.34% to 4.52% on a dry weight basis. Quantitatively, the main okra fatty acids were palmitic acid (29.18-43.26%), linoleic acid (32.22-43.07%), linolenic acid (6.79-12.34%), stearic acid (6.36-7.73%), oleic acid (4.31-6.98%), arachidic acid (ND-3.48%), margaric acid (1.44-2.16%), pentadecylic acid (0.63-0.92%), and myristic acid (0.21-0.49%). Aspartic acid, proline, and glutamic acids were the main amino acids in okra pods, while cysteine and tyrosine were the minor amino acids. Statistical methods revealed how the fatty acid and amino acid contents in okra may be affected by the sampling location. PMID:24171167

  3. Anjitha - A New Okra Variety through Induced Mutation in Interspecific Hybrids of Abelmoschus spp

    Studies on interspecific hybrids of okra between A. esculentus (cultivated type) and A. manihot (wild type) revealed that no useful recombinants were obtained from the conventional breeding programme because of the strong linkage between yellow vein mosaic (YVM) resistance genes and the wild character of A. manihot. This study was aimed at breaking this undesirable linkage through gamma irradiation (100 200, 300 and 400 Gray) of F1 seeds obtained by interspecific hybridization between A. esculentus var. Kiran and A. manihot and further evaluating and selecting high yielding YVM resistant types from the generations segregating until F6M6. The mutagenic effectiveness and efficiency increased with increasing doses of Gamma-rays. In the segregating generations, the irradiated treatments were late flowering and had more leaves, flowers and fruits per plant. Average fruit weight was maximum in 200Gy, while fruit yield was maximum in 400Gy due to larger number of fruits. A few high yielding disease-resistant plants resembling the cultivated plants were obtained in 300Gy which suggested that 300Gy could be the ideal irradiation dose in okra. Superior genotypes selected from F6M6 generation based on yield and YVM resistance were advanced to CYTs and farm trials. Cultivar AE18 outyielded the others and was released as 'Anjitha' during 2006, for cultivation in the Thiruvananthapuram District of Kerala. Anjitha is a high yielding variety having the fruit characters and quality of the cultivated parent A. esculentus var. Kiran combined with the YVM-resistant character of the wild parent A. manihot. (author)

  4. The effects of okra (Abelmoschus esculentus Linn.) on the cellular events associated with Alzheimer's disease in a stably expressed HFE neuroblastoma SH-SY5Y cell line.

    Mairuae, Nootchanat; Connor, James R; Lee, Sang Y; Cheepsunthorn, Poonlarp; Tongjaroenbuangam, Walaiporn

    2015-08-31

    It has been reported that persons carrying the H63D variant in their hemochromatosis (HFE) gene are at increased risk of Alzheimer's disease (AD). We investigated the possibility that okra (Abelmoschus esculentus) and quercetin could mitigate this risk factor by examining its effect on AD-associated cellular events in HFE stably expressing SH-SY5Y cells. Treatment of H63D HFE cells either with okra or quercetin significantly decreased reactive oxygen species (ROS), hydrogen peroxide (H2O2), and protein oxidation compared to untreated cells. The levels of tau phosphorylation at serine-199, serine-202, and serine-396 sites were also significantly decreased when cells were treated with okra. Exposure of the H63D and wild type (WT) cells to iron increased tau phosphorylation, but this response was decreased significantly when cells were treated with okra. The mechanism responsible for these changes appears to be related to decreased glycogen synthase kinase (GSK)-3? activity, an upstream signaling kinase of tau phosphorylation. We also established that okra treatment dramatically decreases intracellular iron levels in H63D cells compared to untreated cells. Our results provide important in vitro data on the effects of okra on various AD-associated cellular processes in H63D variant HFE cells. These results suggest okra may be beneficial in people expressing the H63D variant to reduce the risk of AD and other neurodegenerative diseases related to oxidative stress. Further in vivo studies would help confirm this. PMID:26170247

  5. Residue, dissipation, and safety evaluation of pyridalyl nanoformulation in Okra (Abelmoschus esculentus [L] Moench).

    Saini, Priya; Gopal, Madhuban; Kumar, Rajesh; Gogoi, Robin

    2015-03-01

    A comparative study on residues and rate of dissipation of a new nanoformulation of pyridalyl and commercial pyridalyl was carried out on okra under net house and field conditions. Okra crop was sprayed with commercial and nanoformulation of pyridalyl at recommended (75 g a. i./ha) and double the recommended dose (150 g a. i./ha) at the time of fruiting. Quantitation of residues of pyridalyl in okra was done by ultra high performance liquid chromatography over a period of 15 days, and recovery of the method ranged from 79 to 87 %. The half life calculated from the dissipation pattern of pyridalyl on okra for commercial and developed nanoformulation proved that residues of nanopyridalyl did not persist much longer than that of conventional formulation in net house as well as in field trials. The risk quotient value of pyridalyl in okra was significantly lower than 1, implying its negligible risk to the humans. PMID:25694033

  6. The effects of color plastic mulches and row covers on the growth and yield of okra

    Okra [Abelmoschus esculentus (l.) Moench'Clemson Spineless'] was grown on an Orangeburg sandy loam soil in shorter, AL. Okra was direct seeded in single rows. The experiment consisted of twelve experimental treatments as follows: (1) Black plastic mulch (BPM) + spunbonded row cover (RC), (2) BPM, (3...

  7. Decontamination of sliced and powdered okra (Abelmoschus esculentus) and some aspects of nutrient quality before and after gamma irradiation

    Food security in Africa is crucial for survival of the increasing population. However, agricultural produce in the field are drastically reduced along the food pipeline (from farm gate to the consumer's table) by bioderioagents including microorganisms. Okra (Abelmoschus esculentus (L) Moench) is one such farm produce of economic importance in Ghana with a great potential of providing essential nutrients and vitamins in our diet. The high moisture content of the fresh fruit makes it susceptible to microbial deterioration in a short time. Dehydration methods (sun-drying and solar drying) are practiced worldwide but in Africa, this is attended by contamination by aeromycoflora and other agents such as insect eggs and larvae. In addition are physical and chemical contaminants. In this thesis, the mycoflora and Total Aerobic Bacteria load of market samples and solar-dried okra (Clemson spineless and Legon Finger) chips and powder were ascertained with the view to documenting toxin-producing fungal species and update the resident mycoflora and bacteria load. Insects resident in the samples were detected by the hidden infestation technique; mycoflora was determined by the decimal serial dilution method on different media and Total Aerobic Bacteria population was determined on Plate Count Agar at 320C for 48hr. In order to establish storage stability of the okra, the chips and powdered samples were placed in glass desiccators with glycerol: water mixtures providing Environmental Relative Humidities of 20, 55, 65, 75, 85 and 95% representative of the Ghanaian Tropic Conditions to undergo sorption at the same temperature. Gamma irradiation doses (0, 5, 10, 20kGy) were used as a preservation process to decontaminate resident mycoflora and total aerobic bacteria. This was supplemented by an in vitro study in the radio-resistance of six selected resident fungi (Aspergillus; Penicllium spp). The veracity of the dry okra supporting growth of selected Aspergillus and Penicillium species was carried out in liquid cultures (okra meal broth and okra meal broth amended with glucose) using the conventional oven dry weight method at 300C for 5 days. The ability of Aspergillus flavus to produce aflatoxins B1, B2, G1, G2 in an artificially inoculated 'black' and 'white' market samples of okra powder was studied using the current Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) to quantify aflatoxins with post column derivatisation (PCD) involving bromination. Elemental composition (Cd, Cu, Pb, Ni, Zn, Mn, Na, K, Ca, Fe) was analysed by Atomic Absorption Spectrometry and Flame Photometer methods. A Bench Nuclear Magnetic Resonance equipment determined percentage moisture and fat concurrently; crude protein was ascertained by the Kjeldahl method, total ash was determined by the dry ashing method using Carblolite Eurotherm CW Furnace. Vitamin C was determined by the AOAC (2000) Iodometric Titration method and pH was estimated by AOAC (2000) method, while Brabender Viscograph measured viscosity of powdered samples of okra and Brookfield Viscometer was employed for the okra chips. Colour change was determined on L*a*b* system using a Minolta CR-30 Chromameter. The chips of Clemson spineless and Legon finger as well as the 'black' and 'white' okra powder were hygroscopic and equilibrated at 20-95% ERH with 6-8 days. Samples were visibly mouldy after 6-10 days and harboured a miscellany of fungal genera (Aspergillus, Penicillium, Pullularia, Fusarium, Cladosporium, Trichoderma, Paecilomyces, Rhodotorula sp). Potential toxin-producing fungi (A. flavus, A. alutaceus, A. sulphureus, P. digitatum, P. citrinum, P. brevicompactum, F. verticillioides, P. expansum) variably contaminated the samples. The Cigarette beetle, Lasioderma serricone was recorded for the first time in 'white' okra powder but not the remaining samples. The insect showed mycophagy, ingesting spores of the Mucorales. The okra fruit was a good medium for vegetative growth of the resident fungi. A dose of 10kGy significantly (p?0.05) eliminated resident mycoflora and reduced total aerobic bacteria (TAB) count by 2-3 log cycles. Storage in medium dense polyethylene pouches maintained this quality. In vitro radiation response of four resident fungi (A. niger, A. alutaceus, P. digitatum, P. brevicompactum) confirmed the efficacy of 10kGy to kill the spores. A dose of 10kGy also killed A. flavus spores artificially inoculated 'black' and 'white' okra market samples but the strain of A. flavus formed only aflatoxin B1 (1.99-3.03μg/kg) in the control and the irradiated powders (5-20kGy) indicating that the market samples already contained the toxin and require attention by health authorities. Irradiation treatments (0-20kGy) did not significantly (p?0.05) affect the pH of the samples (pH 5.86-6.04) after 3 months storage and did not also influence the L* (lightness) and b* (yellow/blue) colour of the treated powder and chips. The physical form of the samples (chips or powder) influenced the L*a*b* values. Ash and Crude protein contents were not affected (p≥0.05) by increasing radiation, but the type of okra variety significantly (p≤0.05) influenced treatment means. Irradiation significantly (p≤0.05) decreased vitamin C content and viscosity of the samples with increasing dose. Storage for three months did not affect these parameters as compared to the control. Practical implications of these findings are discussed. (au)

  8. Proximate, mineral, and antinutrient compositions of indigenous Okra (Abelmoschus esculentus) pod accessions: implications for mineral bioavailability.

    Gemede, Habtamu Fekadu; Haki, Gulelat Desse; Beyene, Fekadu; Woldegiorgis, Ashagrie Z; Rakshit, Sudip Kumar

    2016-03-01

    The promotion and consumption of indigenous vegetables could help to mitigate food insecurity and alleviate malnutrition in developing countries. Nutrient and antinutrient compositions of eight accessions of Okra Pods were investigated. Molar ratios and mineral bioavailability of Okra pod accessions were also calculated and compared to the critical values to predict the implications for mineral bioavailability. Proximate and mineral composition of Okra pod accessions were determined using standard methods of Association of Official Analytical Chemists. The result of the study revealed that the proximate composition (g/100 g) in dry weight basis was significantly (P potassium (122.59-318.20), zinc (3.83-6.31), phosphorus (25.62-59.72), and sodium (3.33-8.31) on dry weight bases. The Okra Pods of "OPA#6" accession contained significantly higher amounts of crude protein, total ash, crude fat, calcium, iron, and zinc than all other accessions evaluated in this study. The results of antinutrients analysis showed that, except phytate, tannin, and oxalate contents of all the accessions were significantly (P bioavailability of calcium, iron, and zinc in these accessions could be high. The results of the study revealed that Okra pod contain appreciable amount of vital nutrients like protein, fiber, calcium, iron, and zinc and low in antinutrient contents with high mineral bioavailability. Therefore, increase in the production and consumption of these nutrient-rich indigenous Okra pods will help to supplement/formulate the diets and alleviate the problems associated with malnutrition in the country. PMID:27004112

  9. Dissipation of flubendiamide in/on okra [Abelmoschus esculenta (L.) Moench] fruits.

    Das, Shaon Kumar; Mukherjee, Irani; Das, Suvo Kumar

    2012-03-01

    A field experiment was undertaken at Indian Agricultural Research Institute, New Delhi during kharif (rainy season) in the year 2010 to evaluate the residue persistence of flubendiamide in/on okra fruits following foliar application of Belt 39.35% SC formulation at 24 (standard dose) and 48 (double dose) g a.i. ha(-1). After HPLC analysis study revealed that residues of flubendiamide in/on okra persisted till 5th and 7th day after the last spray at standard and double dose, respectively. The residues of flubendiamide were reported as parent compound, and des-iodo flubendiamide, a metabolite (photo product) of flubendiamide, was not detected in/on okra at any time during the study period. The initial deposits of 0.28 and 0.53 μg g(-1) in/on okra fruits reached below determination level of 0.01 μg g(-1) on the 7th and 10th day at standard and double dose, respectively. The half life of flubendiamide in/on okra fruits ranged from 4.7 to 5.1 days at standard and double dose, respectively. Soil sample collected from the treated field on the 15th day after the last spray revealed residues of flubendiamide or its metabolite below determination level (0.01 μg g(-1)) at single and double dose. PMID:22147083

  10. INFLUENCE OF COVER CROPS AND SOIL AMENDMENTS ON OKRA (ABELMOSCHUS ESCULENTUS L.) PRODUCTION AND SOIL NEMATODES

    A pot experiment to determine the effects of summer cover crops and soil amendments on okra yield and population densities of various soil nematode taxa was conducted in two consecutive growing seasons in a subtropical region. Two cover crops, sunn hemp (Crotalaria juncea) and sorghum sudangrass (So...

  11. Okra (Hibiscus esculentus) seed oil for biodiesel production

    Anwar, Farooq; Nadeem, Muhammad [Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040 (Pakistan); Rashid, Umer [Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040 (Pakistan); Department of Industrial Chemistry, Government College University, Faisalabad 38000 (Pakistan); Ashraf, Muhammad [Department of Botany, University of Agriculture, Faisalabad 38040 (Pakistan)

    2010-03-15

    Biodiesel was derived from okra (Hibiscus esculentus) seed oil by methanol-induced transesterification using an alkali catalyst. Transesterification of the tested okra seed oil under optimum conditions: 7:1 methanol to oil molar ratio, 1.00% (w/w) NaOCH{sub 3} catalyst, temperature 65 C and 600 rpm agitation intensity exhibited 96.8% of okra oil methyl esters (OOMEs) yield. The OOMEs/biodiesel produced was analyzed by GC/MS, which showed that it mainly consisted of four fatty acids: linoleic (30.31%), palmitic (30.23%), oleic (29.09%) and stearic (4.93%). A small amount of 2-octyl cyclopropaneoctanoic acid with contribution 1.92% was also established. Fuel properties of OOMEs such as density, kinematic viscosity, cetane number, oxidative stability, lubricity, flash point, cold flow properties, sulfur contents and acid value were comparable with those of ASTM D 6751 and EN 14214, where applicable. It was concluded that okra seed oil is an acceptable feedstock for biodiesel production. (author)

  12. Efecto del raleo sobre el rendimiento de dos variedades de okra (Abelmoschus esculentus (L. Moench, en el Valle de Lerma, Salta, Argentina

    Astorga, R.

    2011-07-01

    Full Text Available The purpose of this paper was to determine the effect of thethinning on fruit yield components in two okra (Abelmoschusesculentus (L Moench varieties. On October 20, 2008, followinga completely random design with factorial arrangement(varieties and thin a total of 40 seeds (two per hill of everyvariety were sown in the experimental unit. Thirty-eight dayslater they were thined, leaving a plant per hill, in each plot. Thefruits were harvested immature from December, 2008 untilMarch, 2009. The measured variables were: total weight offruits per plot during the reproductive period, fruit weight perplot per harvest, weight per fruit, total number of fruits duringreproductive period and number of fruits per harvest. Therewas a trend (P = 0,054 that the total weight and the weight perharvest were highest in the plots without thinning for bothvarieties. The thinning diminished significantly the number oftotal fruits and the number of fruits per harvest in both varieties,because the thinned plots were presenting half of the numberof plants. Finally, there was no significant effect of the thinningon the average weight of the fruit in any of the studiedvarieties.--------------------------------------------------------------------------------------------------El objetivo del presente trabajo fue determinar el efecto del raleo sobre los componentes del rendimiento en dos variedades de okra (Abelmoschus esculentus. El 20 de octubre de 2008, siguiendo un diseño completamente aleatorio con arreglo factorial (variedades y raleo se sembró en cada unidad experimental un total de 40 semillas (dos por golpe de siembra de la variedad correspondiente. A los 38 días, se raleó, dejando una planta por golpe de siembra, en las parcelas indicadas. Los frutos fueron cosechados inmaduros desde diciembre de 2008 hasta marzo de 2009. Las variables analizadas fueron: peso total de frutos por parcela durante todo el ciclo de producción, peso por parcela por cosecha, peso por fruto, número total de frutos durante todo el ciclo de producción y número de frutos por cosecha. Hubo una tendencia (P = 0,054 a que el peso total y el peso por cosecha sean superiores en las parcelas sin raleo para ambas variedades. El raleo disminuyó significativamente el número de frutos totales y el número de frutos por cosecha en ambas variedades, debido a que las parcelas raleadas presentaban la mitad del número de plantas. Por último, no hubo un efecto significativo del raleo sobre el peso promedio del fruto en ninguna de las variedades estudiadas.

  13. Yellow vein mosaic virus resistant hybrids in okra (Abelmoschus esculentus L.) Moench)

    K. Nirosha, P. Irene Vethamoni and V. A. Sathiyamurthy

    2015-01-01

    Seven parents and 42 hybrids of okra were screened for resistance/susceptibility to yellow vein mosaic virus. The parents P1 (AE 64 (White)), P2 (AE 64 (Pink)) and P4 (AE 65 (Pink) were found complete resistance to disease. The parents P3 (AE 65 (White)), P6 (AE 70 (White)) and P7 (AE 71 (White) were found tolerant to disease. The parent P5 (AE 66 (Pink) found susceptible to disease. 42 hybrids are obtained by crossing seven parents in diallel design. Twelve out of 42 hybrids did not show any...

  14. Irrigational impact of distillery effluent on Abelmoschus esculentus L. Okra with special reference to heavy metals.

    Srivastava, Sachin; Chopra, A K

    2014-07-01

    The present study was performed under natural environment to assess levels of different heavy metals in soil and Abelmoschus esculentus plants along with soil microbial population irrigated with five rates of distillery effluent (DE) viz. 10, 25, 50, 75 and 100 % concentration in comparison with control (Bore well water). Results revealed that among various concentrations of DE, irrigation with 100 % DE significantly (P Cr > Cd > Zn > Cu, and for A. esculentus plants, it was in the order Ni > Cr > Cu > Cd > Zn after irrigation with distillery effluent. The enrichment factor value was found maximum for Ni in comparison to other metals at 100 % DE concentration as compared with BWW. However, the values of these metals were below the recommended permissible limit. PMID:24638936

  15. Advances in breeding of okra [Abelmoschus esculentus (L.) Moench.] in India

    Full text: Okra, an important vegetable of the tropics and sub-tropics is very popular in India. Its production is limited by 'yellow vein mosaic virus'. Advances in breeding for resistance to this virus have been made through inter-specific hybridisation as well as mutagenesis. Hybridisation used A. manihot ssp. manihot and ssp. tetraphyllus. New varieties showed 87-146% yield increases over older virus susceptible varieties. The number of fruits increased by 13-30%, virus incidence decreased by 84-99%. An EMS induced mutant 'EMS8' showed a yield increase of 107%, a fruit number increase of 16% and a disease decrease of 99%. The mutant also carries a good amount of resistance to the fruit borer; infestation decreased by 46%. The mutant is the best among the tested varieties for canning, is suitable for dehydration, and can be stored prepacked at room temperature for 6 days. (author)

  16. Quantitative inheritance for fruit traits in inter varietal crosses of okra (Abelmoschus esculentus L. Moench

    Deepak Arora, Salesh Kumar Jindal and T. R. Ghai

    2010-12-01

    Full Text Available Gene effects for important fruit traits of four inter-varietal crosses of okra were estimated by partitioning the means and variances of means of sixbasic generations from each cross into their genetic components to assess the gene action governing the inheritance of fruit yield and earlinessrelated traits in spring and rainy season. The additive, dominance and digenic non-allelic gene interactions were observed to govern most of thefruit traits. The non-additive gene effects were more pronounced than additive ones for most of the traits in both the environments. The evidenceof duplicate type of epistasis has been obtained for all the characters in different crosses in both the seasons. Thus for developing high yieldingokra cultivars, recurrent selection in biparental progenies would help in exploiting the duplicate type of non-allelic interactions and allowrecombination and concentration of genes having cumulative effects in population.

  17. Ferti-irrigational effect of paper mill effluent on agronomical characteristics of Abelmoschus esculentus L. (Okra).

    Kumar, Vinod; Chopra, A K

    2013-11-15

    The ferti-irrigational effect of an agro-based paper mill effluent on Abelmoschus esculentus (var. IHR-31) was investigated. Different doses of paper mill effluent viz. 5, 10, 25, 50, 75 and 100% were used for fertigation ofA. esculentus along with bore well water (control). The study revealed that paper mill effluent had significant (p 0.05) changes in WHC and bulk density of the soil were observed after irrigation with paper mill effluent. The agronomical performance of A. esculentus was increased from 5 to 25% and decreased from 50 to 100% concentration of paper mill effluent as compared to control in both seasons. The heavy metals concentration was increased in A. esculentus from 5 to 100% concentrations of paper mill effluent in both seasons. Biochemical components like crude proteins, crude fiber and crude carbohydrates were found maximum with 25% paper mill effluent in both seasons. The order of Contamination Factor (Cf) of various heavy metals was Cr > Cd > Mn > Zn > Cu for soil and Zn > Mn > Cu > Cr > Cd for A. esculentus plants after fertigation with paper mill effluent. Therefore, paper mill effluent can be used as a biofertigant after appropriate dilution to improve yield of A. esculentus. PMID:24511684

  18. Yellow vein mosaic virus resistant hybrids in okra (Abelmoschus esculentus L. Moench

    K. Nirosha, P. Irene Vethamoni and V. A. Sathiyamurthy

    2015-03-01

    Full Text Available Seven parents and 42 hybrids of okra were screened for resistance/susceptibility to yellow vein mosaic virus. The parents P1 (AE 64 (White, P2 (AE 64 (Pink and P4 (AE 65 (Pink were found complete resistance to disease. The parents P3 (AE 65 (White, P6 (AE 70 (White and P7 (AE 71 (White were found tolerant to disease. The parent P5 (AE 66 (Pink found susceptible to disease. 42 hybrids are obtained by crossing seven parents in diallel design. Twelve out of 42 hybrids did not show any symptom of YVMV and were P1 x P2, P1 x P3, P1 x P4, P1 x P5, P1 x P7, P2 x P1, P2 x P4, P4 x P1, P4 x P2, P4 x P3, P4 x P5 and P4 x P7. Eight hybrids viz., P2 x P3, P3 x P1, P3 x P2, P3 x P4, P4 x P6, P5 x P1, P6 x P4 and P7 x P4 were highly resistant to the YVMV disease with the incidence of 7.14, 3.57, 7.14, 7.14, 7.14, 3.57, 3.57 and 3.57 per cent respectively at 105 DAS.

  19. Genetic analysis for fruit yield and yield attributes in okra (Abelmoschus esculentus L. Moench

    P. K. Akotkar and D. K. De

    2014-12-01

    Full Text Available The components of gene effects for yield and its components in okra were studied using generation mean analysis from six generations (P1, P2, F1, F2, BC1, and BC2 derived from six different genotypes. Joint scaling test was applied to detect the presence of epistasis. Epistasis was detected in both the cases. Prevalence of duplicate type of epistasis was observed in all the cases except number of fruits/ plant, fruit length in C1 (IC 3307 x IC 433645, fruit diameter, inter node length in C2 (IC- 43736 x Parbhani Kranti, fruit length, fruit diameter and fruit weight in C3 (IC-342075 X IC-332453. Additive, dominance and epistatic gene effects contribute significantly to the inheritance of various yield characters studied. The joint scaling test confirmed the inadequacy of the additive dominance model in most of the crosses for majority of the characters and indicated the presence of epistasis. Low narrow sense heritability values for most of the characters indicating that these characters are more influenced by the environment and they cannot be improved by simple selection.

  20. Nutritive Composition and Properties Physico-chemical of gumbo (Abelmoschus esculentus L. Seed and Oil

    J.M. Nzikou

    2010-01-01

    Full Text Available Chemical and physical properties of mature gumbo (Abelmoschus esculentus L. seeds fromImpfondo, in North Congo-Brazzaville were evaluated. The chemical properties evaluated were moisture, crudeprotein, crude oil, crude ash, crude fiber, and crude energy. The oil from A.esculentus seeds was extracted usingtwo oils extraction methods with petroleum ether (Soxlhet and extraction with a mixture ofchloroform:methanol (1:1 (Blye and Dyer.The oil concentration ranged from 24.90% (Soxlhet to 21.98%(Blye & Dyer. The minerals, viscosity, acidity, saponification value, iodine value, fatty acid methyl esters,unsaponifiable matter content, peroxide value, activation energy and differential scanning calorimetry w eredetermined. Abelmoschus esculentus L. seeds have ash content of 5.68% (with the presence of followingminerals: Ca, M g, K and N a. The oil was found to contain high levels of unsaturated fatty acids, especiallyoleic (up to 24.89% and linoleic (up to 42.78%. Abelmoschus esculentus L. oil can be classified in theoleic-linoleic acid group. The dominant saturated acid w as palmitic (up to 25.79%. Abelmoschus esculentusL. seeds were also founded to contain high levels of crude protein (24.85%. The content of insaponifiables is1.53%. Taking into account these results, the gumbo (Abelmoschus esculentus L. finds its applications in thefood and cosmetic industry.

  1. Effect of Storage Conditons on Some Physico-Chemical and Microbiological Properties of Fresh Okra (Abelmoschus esculenta Fruits

    D.I. Gernah

    2012-07-01

    Full Text Available The effects of storage conditions on some physico-chemical and microbiological qualities of fresh okra were determined and compared. Fresh okra fruits were stored in open plates and sealed polyethylene bags in the refrigerator (F1, F2, on the shelf in the laboratory (S1, S2 and in the open sun (O1, O2 respectively for a period of nine (9 days. The moisture content, viscosity, microbiological composition and sensory properties of the stored fruits were determined on a daily basis during the period of storage. The moisture content and viscosity decreased during storage from 92.00% and 700 cP in fresh okra to 35.50% and 100 cP in okra stored in open plates in the sun (O2, respectively. On the other hand, bacterial count increased from 2.27x104 CFU/mL in fresh okra to 2.72x105 CFU/mL in okra stored in polyethylene bag in the sun (O2, while okra stored in open plates on the shelf (S1 had the highest mould count of 9.0x104 CFU/mL. Okra stored in polyethylene bag in the refrigerator (F2 had the highest moisture content of 84.50%, viscosity of 440 cP and the lowest bacterial and mould counts of 1.15x104 and 4.0x103 CFU/mL, respectively. Mean sensory scores in respect of colour, smell, texture and general acceptability showed that samples stored in the refrigerator scored higher in all attributes and had higher acceptability at the end of the storage period, which compared favourably with fresh okra fruits.

  2. Nutritive Composition and Properties Physico-chemical of gumbo (Abelmoschus esculentus L.) Seed and Oil

    J.M. Nzikou

    2010-01-01

    Chemical and physical properties of mature gumbo (Abelmoschus esculentus L.) seeds fromImpfondo, in North Congo-Brazzaville were evaluated. The chemical properties evaluated were moisture, crudeprotein, crude oil, crude ash, crude fiber, and crude energy. The oil from A.esculentus seeds was extracted usingtwo oils extraction methods with petroleum ether (Soxlhet) and extraction with a mixture ofchloroform:methanol (1:1) (Blye and Dyer).The oil concentration ranged from 24.90% (Soxlhet) to 21....

  3. Comparative Efficacy of Insecticides, Insect Growth Regulator and M.Y. Strategy Against Insect Pets of Okra (Abelmoschus esculentus

    Muhammad Tariq

    2000-01-01

    Full Text Available The efficacy of Azodrin and Pirate (insecticides, Cascade (insect growth regulator and Pressurized water spray (M. Y. strategy was evaluated against insect pests of okra. Cascade was found to be the best efficient against Helicoverpa armigera and Earias spp. Pirate showed maximum efficacy against Amrasca biguttula biguttula, whereas, against Bemisia tabaci, the most effective treatment was Azodrin followed by M.Y. strategy.

  4. Influence of wastewater application and fertilizer use on growth, photosynthesis, nutrient homeostatis, yield and heavy metal accumulation in okra (Abelmoschus esculentus L. Moench).

    Faizan, Shahla; Kausar, Saima; Akhtar, Neelima

    2014-05-01

    The scarceness of freshwater assets is a serious problem in semi-arid zones and marginal quality water is increasingly being used in agriculture. This study aimed at evaluating the physico-chemical and biological risks on irrigated soils of treated wastewater, the nutrient supply and the effect on okra plant. A pot experiment based on completely randomized block design was conducted with Treated Wastewater (TW) and inorganic fertilizers to observe a comparative effect on biochemical characters using Okra var. Nidhi. The physico-chemical analysis of the TW showed that it was rich in total suspended and dissolved solids with large amount of BOD and COD. The higher amount of Cl-, Ca++, Mg++ and K+ were also present in the effluent. The heavy metal (Cd, Cr, Ni and Pb) content in TW is comparatively more than groundwater (GW). The values of these heavy metals were slightly higher in the soil irrigated with TW. The effluent severely affects crop plants and soil properties when used for irrigation. The growth parameters, photosynthetic characteristics, chlorophyll content, yield and nutrient homeostatis were analyzed during different growth periods in all treatments. All the parameters were found to increase due to wastewater application. Among the fertilizer treatments, N120 proved optimum, N90 deficient and N150 proved as luxury dose. The seeds accumulated Cd and Ni but their level was under permissible limits. Thus, it may be concluded that wastewater may be used profitably for the cultivation of okra. PMID:26030995

  5. Influence of Wastewater Application and Fertilizer use on Growth, Photosynthesis, Nutrient Homeostatis, Yield and Heavy Metal Accumulation in Okra (Abelmoschus esculentus L. Moench

    Neelima Akhtar

    2014-01-01

    Full Text Available The scarceness of freshwater assets is a serious problem in semi-arid zones and marginal quality water is increasingly being used in agriculture. This study aimed at evaluating the physico-chemical and biological risks on irrigated soils of treated wastewater, the nutrient supply and the effect on okra plant. A pot experiment based on completely randomized block design was conducted with Treated Wastewater (TW and inorganic fertilizers to observe a comparative effect on biochemical characters using Okra var. Nidhi. The physico-chemical analysis of the TW showed that it was rich in total suspended and dissolved solids with large amount of BOD and COD. The higher amount of Cl¯, Ca++, Mg++ and K+ were also present in the effluent. The heavy metal (Cd, Cr, Ni and Pb content in TW is comparatively more than groundwater (GW. The values of these heavy metals were slightly higher in the soil irrigated with TW. The effluent severely affects crop plants and soil properties when used for irrigation. The growth parameters, photosynthetic characteristics, chlorophyll content, yield and nutrient homeostatis were analyzed during different growth periods in all treatments. All the parameters were found to increase due to wastewater application. Among the fertilizer treatments, N120 proved optimum, N90 deficient and N150 proved as luxury dose. The seeds accumulated Cd and Ni but their level was under permissible limits. Thus, it may be concluded that wastewater may be used profitably for the cultivation of okra.

  6. Effect of Monocrotophos and Methamidophos on Jassid, Amrasca devastans
    (Distant in Different Okra (Abelmoschus esculentus L. Cultivars

    Munir Ahmad

    2001-01-01

    Full Text Available Two commonly used insecticides, Methamidophos 60SL @ 1000ml/ha and Monocrotophos 40WSC @ 1500 ml/ha were evaluated on Amrasca devastans L. during 1999 on Pusa Swani, Parbhani Kranti, Sabz Pari and Karishma okra varieties. Both insecticides provided effective control of Jassid and kept the population below economic threshold level up to ten days after insecticidal application. However, Methamidophos 60SL proved better on Pusa Swani as compared to Monocrotophos 40WSC, which decreased jassid population on Parbhani Kranti, Sabz Pari and Karishma, respectively.

  7. Effects of Green Manures on Growth, Yield and Quality of Green Okra (Abelmoschus esculentus L. Har Lium Cultivar

    Chutichudet Benjawan

    2007-01-01

    Full Text Available Abstract: This green manure experiment with the use of okra crop as indicator plant was carried out at Mahasarakham University Experimental Farm, Mahasarakham province, Northeast Thailand during May to September 2005 to investigate four types of green manure legume crops on growth, yield and quality of edible fresh pods of okra crop when grown on Roi-Et soil series (Oxic Paleustults. The four types of green manure plants include Jack bean, Cowpea, Green gram, and Giant sensitive plant. The experiment consisted of five treatments, i.e., T1 (Control, T2 (Jack bean, T3 (Cowpea, T4 (Green gram and T5 (Giant sensitive plant. The experiment was laid in a Completely Randomized Design (CRD with four replications. The results showed that Roi-Et soil series (Oxic Paleustults contained some considerable mean values of organic matter (1.64-1.66% but soil available phosphorus and exchangeable potassium were relatively low, particularly potassium. Green manures of the four legume plants slightly improved soil property of the Roi-Et soil series (tested at the end of the experimental period. The most effective green manure on stem diameter, bushy diameter, leaf numbers plant- 1 and leaf area of the fifth leaf of the okra plants, in most cases, was found with Jack bean and Cowpea ranked the second. However, in most cases, Cowpea gave a similar effect as that of Green gram and Giant sensitive plant. Pod length and weight pod- 1, pod diameter and edible fresh pod yields (5,941.86 kg ha- 1 were highest with Jack bean green manure treatment (T2, whilst the rest, in most cases, were similar. Green manure treatments gave highly significant effect on total soluble solids of pods over the control treatment, whilst total acidity, fibre and pectin contents were unaffected by green manure treatments. Green manure of Jack bean was the best legume crop for green manure to be used in improving soil fertility, particularly for Roi-Et soil series (Oxic Paleustults.

  8. Effect of gamma rays on fruit weight and number of seeds in Abelmoschus esculentus (L.) Moench and Momordica charantia L

    Among 5,15,30,60,90 and 120 kR doses of gamma rays, lower doses showed stimulatory effects on fresh and dry weight of fruit, while higher doses proved inhibitory in Abelmoschus esculentus and Momordica charantia. Abortion of mature seeds was also higher at 30 kR and above doses. (author). 12 refs., 2 tabs

  9. PRE-SOWING SEED BIO-PRIMING IN OKRA: RESPONSE FOR SEED PRODUCTION

    A K RAI

    2014-06-01

    Full Text Available Seeds of eight varieties of okra: Lalu, Arka Anamika, Ramya, Satsira, Lady Luck, Debpusa Jhar, Japani Jhar and Barsha Laxmi were bio-primed with Trichoderma viride and Pseudomonas fluorescens, and sown in the field in Split-plot Design with three replications along with the unprimed control in two consecutive summer seasons of 2011 and 2012 to assess the variation in response of individual varieties for seed yield and its attributes. Trichoderma viride was improved plant length as 108.21cm and 112.25 cm for variety Arka Anamika, maximum pod length i.e. 19.01 and 19.21 as well as in pod diameter as 16.64 mm and 16.85 mm for Lalu variety in first and second year respectively. Overall seed yield per plant was as maximum as 49.14g and 51.58g in 2011 and 2012 respectively for Lalu, while it was lowest for Lady Luck (24.13g and 25.69g in both the years. Expression for response towards individual bio-inoculants for individual varieties varied, which indicate the existence of genotypic response in enhancing the seed yield of individual varieties after pre-sowing bio-primed of seeds.From these findings it can be concluded that the bio-priming with compatible bio-agents will enhance the plant growth and yield attributes.

  10. Urea polymer coated with controlled release in physiological quality okra seeds

    Miquéias de Oliveira Assis

    2015-03-01

    Full Text Available The objective of this work was to evaluate the influence of urea coated with controlled release polymer in the seed quality of okra cultivar Santa Cruz 47.The experimental design was completely randomized with four replications.The experiment consisted of two parts. At first, we evaluated four doses of nitrogen (0, 48, 96, and 144 kg ha-1 applied using as a source, a commercial mixture containing 30% urea and 70% common urea polymer controlled release. In the second part, we tested the effect of two nitrogen sources, urea and conventional urea polymer controlled release at a dose of 120 kg ha-1. In the first part, the treatment means were compared by regression analysis and the second, the “t” test at 5%. To evaluate the physiological quality of seeds, the following tests and / or determinations were carried out: moisture, seedling emergence, speed of emergence index, seedling length, fresh and dry weight of seedlings, and electrical conductivity. The seed quality of okra cultivar Santa Cruz 47, reduces to the application of doses of conventional commercial mixture containing urea and urea polymer coated controlled release. The nitrogen sources used did not affect the physiological quality of seeds of okra.

  11. Effect of different levels of nitrogen alone and in combination with constant doses of phosphorus and potassium on growth and yield of Okra (Abelmoschus esculentus L. Cv. T-13 under the agro-climatic conditions of Mingora, Swat

    Humayun Khan

    2000-01-01

    Full Text Available Investigation were undertaken to study the effect of different level of nitrogen (0,100,120,140 kg ha-1 alone and in combination with constant doses of phosphorous (90 kg ha-1 on growth and yield of Okra (Abelmoschus esculentus L. Cv.T-13 at Agriculture Research Station (North Mingora, Swat during 1997. Maximum plant height (185 cm, number of pods per plant (24.59 pod length (12.63 cm, and the highest yield per hectare (16950.79 kg was recorded on the plots fertilized with 120:90:60 kg ha-1. No significance differences were observed in days to emergence, flowering, and first picking respectively.

  12. Antioxidant and Anti-Fatigue Constituents of Okra.

    Xia, Fangbo; Zhong, Yu; Li, Mengqiu; Chang, Qi; Liao, Yonghong; Liu, Xinmin; Pan, Ruile

    2015-10-01

    Okra (Abelmoschus esculentus (L.) Moench), a healthy vegetable, is widely spread in tropical and subtropical areas. Previous studies have proven that okra pods possess anti-fatigue activity, and the aim of this research is to clarify the anti-fatigue constituents. To achieve this, we divided okra pods (OPD) into seeds (OSD) and skins (OSK), and compared the contents of total polysaccharides, total polyphenols, total flavonoids, isoquercitrin, and quercetin-3-O-gentiobiose and the antioxidant activity in vitro and anti-fatigue activity in vivo between OSD and OSK. The contents of total polyphenols and total polysaccharides were 29.5% and 14.8% in OSD and 1.25% and 43.1% in OSK, respectively. Total flavonoids, isoquercitrin and quercetin-3-O-gentiobiose (5.35%, 2.067% and 2.741%, respectively) were only detected in OSD. Antioxidant assays, including 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, ferric reducing antioxidant power (FRAP) and reducing power test, and weight-loaded swimming test showed OSD possessed significant antioxidant and anti-fatigue effects. Moreover, biochemical determination revealed that that anti-fatigue activity of OSD is caused by reducing the levels of blood lactic acid (BLA) and urea nitrogen (BUN), enhancing hepatic glycogen storage and promoting antioxidant ability by lowering malondialdehyde (MDA) level and increasing superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) levels. These results proved okra seeds were the anti-fatigue part of okra pods and polyphenols and flavonoids were active constituents. PMID:26516905

  13. Growth, yield and seed production of okra as influenced by different growth regulators

    Plant growth regulators (PGRs) affect various aspects of plant physiology, mainly vegetative and reproductive traits including yield and seed production. Therefore, different concentrations (0, 50, 100 and 200 ppm) of gibberellic acid (GA3) and naphthalene acetic acid (NAA), alone or in different combinations were sprayed on okra plants at 2-true leaf stage, to ascertain their impact on plant growth, pod production, seed yield and seed quality. All variables regarding vegetative and reproductive growth were significantly influenced by different concentrations of the growth regulators except number of days taken to flowering. Growth regulators were less effective when applied individually as compared to their combined use; however, performance of plants treated with individual PGR was better than the untreated plants. The number of leaves plant/sup -1/ and plant height was higher in plants when sprayed with GA/sub 3/ and NAA at the rate 200+100 ppm as well as with GA/sub 3/ and NAA at the rate 200+200 ppm. The number of pods plant/sup -1/, pod length, pod fresh and dry weight, seed yield and seed quality (in terms of germination percentage and 1000-seed weight) was maximum in plants receiving foliar spray of both GA3 and NAA at the rate 200+200 ppm. These results signify the role of GA/sub 3/ and NAA in okra pod production for fresh consumption as well as for seed yield. (author)

  14. Influence of Some Soil Amendments on Insect Pest Infestation and Damage to Okra (Abelmoschus esculentus (L. Moench in Umudike, Abia State

    2007-01-01

    Full Text Available Field experiment to determine the effect of three soil amendments (Poultry manure, goat manure and NPK 15:15:15 fertilizer on insect pest infestation and damage to Okra (Oboro dwarf variety was conducted in 2006 at Umudike, Nigeria. The experiment was laid out in a Randomized Complete Block Design (RCBD with three treatments and control replicated five times. During the experiment, application of poultry manure and goat manure at 50g/plant and 20g/plant of NPK 15:15:15 were applied two weeks after planting in a ring form. Results obtained showed that the application of goat manure and poultry manure generally, significantly reduced the prevalence of Podagrica spps with regards to plant height and leaf area of Okra. The application of NPK 15:15:15 may have promoted vegetative growth of Okra, making it more succulent and thereby enhancing high pest attack, survival and damage of the Okra. Hence, increasing the application rates of the different soil amendments may have significant increase on the yield as well as reduce pest invasion.

  15. Chemical, physical and biological features of Okra pectin

    Sengkhamparn, N.

    2009-01-01

    In Thailand, many plants have been used as vegetables as well as for traditional medicine. Okra, Abelmoschus esculentus (L.) Moench, is an example of such a plant. Examples for the medical use are treatment of gastric irritation, treatment of dental diseases, lowering cholesterol level and preventing cancer. These biological activities are ascribed to polysaccharide structures of okra in particular pectin structures. However, the precise structure of okra pectins and also of other polysa...

  16. Qualidade de sementes de quiabeiro em função da idade e do repouso pós-colheita dos frutos Okra seed quality as a function of age and fruit post harvest rest

    Márcia Maria Castro

    2008-10-01

    Full Text Available O trabalho foi conduzido na FCA-UNESP, em Botucatu (SP, objetivando-se estudar o efeito da idade do fruto e o período de repouso pós-colheita sobre a qualidade de sementes de quiabeiro cv. Santa Cruz-47 (Abelmoschus esculentus Moench.. No dia da antese, as flores foram etiquetadas e os frutos foram colhidos com 34, 41, 48 e 55 dias após a antese (DAA. Para cada idade, em metade dos frutos as sementes foram extraídas imediatamente e a outra metade ficou em repouso por quatorze dias, antes da extração das sementes. Para verificar a qualidade das sementes os seguintes testes foram realizados: teor de água no dia da extração das sementes, massa de 100 sementes, teste de germinação, primeira contagem do teste de germinação e condutividade elétrica. Pode-se concluir que o armazenamento para qualquer idade do fruto melhora o vigor das sementes. Para germinação final, o armazenamento é favorável quando os frutos são colhidos ainda novos (34 e 41 DAA.The work was carried out at FCA-UNESP, in Botucatu (SP, with the aim of studying the effect of fruit age and post harvest rest on quality of okra seeds cv. Santa Cruz-47 (Abelmoschus esculentus Moench.. At the day of the anthesis, the flowers were labeled and fruits were picked with 34,41,48 and 55 days after the anthesis (DAA. For each age, in half of the fruits the seeds were immediately extracted and the another half was stored for fourteen days before seed extraction. To verify the quality of seeds the following tests were accomplished: seed water content at extraction, weight of 100 seeds, germination test, first counted of germinated seeds and eletrical conductivity. It may be concluded that the fruit storage for any fruit age increases seed vigour, and for final germination, the rest is favorable when fruits are picked still new (34 and 41 DAA.

  17. Germination and hardseedness of seeds in okra elite lines Germinação e dureza de sementes em linhas elite de quiabo

    Luis Felipe V. Purquerio; Antonio A. do Lago; Francisco Antonio Passos

    2010-01-01

    The occurrence of hardseedness and the low percentage of seed germination are major challenges when growing okra. For this reason, five elite lines of okra developed at the Campinas Agronomic Institute (IAC), three with angular pods (lines 8.1, 20.1.2 and 47.1) and two with cylindrical pods (lines 13.1.2 and 20.1.R), were evaluated for seed quality and compared to two commercial seed samples (Horticeres Sementes and Sakata Seed Sudamerica) of cultivar Santa Cruz 47. Seeds were field produced ...

  18. GENETIC DIVERSITY OF OKRA (ABELMOSCHUS ESCULENTUS L. GENOTYPES FROM DIFFERENT AGRO-ECOLOGICAL REGIONS REVEALED BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS

    Naser M. Salameh

    2014-01-01

    Full Text Available This study was carried out to assess the genetic diversity in 48 genotypes and accessions using eight Amplified Fragment Length Polymorphism (AFLP primer-pairs. The eight selected AFLP primer-pairs generated a total of 150 polymorphic loci. Using the generated AFLP data, the Un-Weighted Pair Group Method with Arithmetic Average (UPGMA ordered the genotypes into six groups based on Dice similarity coefficient. The range in taxonomic distance was from 0.23 to 1.0. Each cluster was found to have genotypes and accessions from different regions and climate and sometimes different continents. The size range of the loci ranged from 87-662 bp. Great variation between the genotypes and accessions in the different cluster could be of high value as the genetically diverse okra genotypes represent a potentially valuable source for improved pathogen and pest resistance.

  19. Pre-germinative treatments in okra seeds in different stadiums of fruit maturationTratamentos pré-germinativos em sementes de quiabo em diferentes estádios de maturação do fruto

    Célia Maria Peixoto de Macedo

    2011-12-01

    Full Text Available The objective of this work was to evaluate the influence of the pre-soak and of the thermotherapy in the germination and in the energy of the okra seeds (Abelmoschus esculentus in different stadiums of maturation of the fruit. The design used was the entirely randomized with four repetitions, in a factorial outline 5 x 9, five maturation stadiums (green, semi-hard, hard, dry and dehiscent and nine treatments pre-germination: pre-soak for immersion of the seeds in water to the temperature of 30ºC for 6, 12 and 24 hours; immersion of the seeds in water to 40, 50, 60 and 70ºC for 3 minutes; thermotherapy for immersion of the seeds in water to the ebullition temperature (97ºC until cooling and intact seeds (control. The seeds were sowed in paper-towel rolls and they were maintained in camera type BOD regulated to 20-30ºC and photoperiod of 8-16 hours. The okra seeds originating from dry and dehiscent fruits presented larger germination performance. The pre-germination treatments more effective to accelerate the germination of seeds obtained from dry and dehiscent fruits are the pre-soak for 6 hours and thermotherapy at 60 °C for 3 minutes, respectively.O objetivo deste trabalho foi avaliar a influência da pré-embebição e da termoterapia na germinação e no vigor das sementes de quiabo (Abelmoschus esculentus em diferentes estádios de maturação do fruto. O delineamento foi o inteiramente casualizado com quatro repetições, num esquema fatorial 5 x 9, cinco estádios de maturação (verde, semi-duro, duro, seco e deiscente e nove tratamentos pré-germinativos: pré-embebição por imersão das sementes em água à temperatura de 30ºC por 6, 12 e 24 horas; imersão das sementes em água a 40, 50, 60 e 70ºC por 3 minutos; termoterapia por imersão das sementes em água à temperatura de ebulição (97ºC até esfriar e sementes intactas (testemunha. As sementes foram semeadas em rolos de papel-toalha que foram mantidos em câmara tipo BOD regulada a 20-30ºC e fotoperíodo de 8-16 horas. As sementes de quiabo oriundas de frutos secos e deiscentes apresentam maior desempenho germinativo. Os tratamentos pré-germinativos mais eficientes para acelerar a germinação das sementes obtidas de frutos secos e deiscentes são a pré-embebição por 6 horas e a termoterapia a 60ºC por 3 minutos, respectivamente.

  20. Differential Responses for Harvesting Times and Storage on Hardness of Different Varieties of Okra

    Ghadir MOHAMMADI

    2011-11-01

    Full Text Available Okra seed hardness results in slow and nonuniform germination. This study was conducted to determine the effects of time of harvest and storage on seed germination of four different cultivars of okra (Abelmoschus esculentus L.. The study was carried out at Agricultural faculty of Razi University in years 2009 and 2010. The experimental design was a randomized complete block under factorial arrangement with three replications. Four different cultivars of okra included, �Boiatloy�, �Beloudo�, �Clemsson Seinless� and �Pleas� and six harvesting times of 20-25-30-35-40 and 50 days after flowering were considered in the present study. The results showed that seed germination increased in 2009 from 3.4%, at first harvesting time, to 46.4% at fifth harvesting time, and in 2010 from 2.9%, at the first harvesting time, to 49.7% at fifth harvesting time. Different cultivars varied in seed germination. In 2009 �Plea� and in 2010 �Clemson� cultivars showed the highest germination. Seeds from different parts of the plant also showed different germination rate. In 2009 seeds from the middle part of the plant with 54.3% and in 2010 seed from the lower part with 50.3 % showed the highest germination. In 2009 seeds from the lower part of pod showed more germination, whereas in 2010 seeds from middle part of pod produced higher germination. By increasing time to harvest, the percentage of seed germination after storage highly increased. Different cultivars during storage showed different behaviors on germination. �Beloudo� cultivar produced more seed germination than other varieties after storage, and �Pleas� after storage produced lower seed germination. Seeds from the middle part of plant and middle part of pod showed a higher germination after storage.

  1. Effect of different fertilization and irrigation methods on nitrogen uptake, intercepted radiation and yield of okra (Abelmoschus esculentum L) grown in the Keta Sand Spit of Southeast Ghana

    Danso, E O; Abenney-Mickson, S; Sabi, E B; Plauborg, Finn; Abekoe, M; Ohui Kugblenu, Y; Jensen, Christian Richardt; Andersen, Mathias Neumann

    2015-01-01

    yield of drip fertigated okra was as high as or better than sprinkler irrigated okra with manure spread on the soil, sprinkler and drip with localized manure. In the second experiment, though nitrogen applied was the same (89 kg N ha−1), for all treatments, the highest N uptake, intercepted radiation......Three seasons’ experiments were conducted in the Keta Sand Spit to test if current use of sprinkler irrigation and animal manure can be substituted by water saving drip fertigation with reduced P supply to okra. The treatments compared were: (i) irrigation by sprinkler, fertilized with manure...... spread, (ii) irrigation by sprinkler, fertilized with localized manure, (iii) irrigation by drip, fertilized with localized manure, (iv) irrigation by drip, fertigated with N–K chemical fertilizers (twice during the crop season in the first experiment, weekly in the second and third experiment). Nitrogen...

  2. Protective Effect of Abelmoschus esculentus Against Alloxan-induced Diabetes in Wistar Strain Rats.

    Mishra, Neetu; Kumar, Dileep; Rizvi, Syed Ibrahim

    2016-11-01

    Increased oxidative stress has been shown to play an important role in the etiology and pathogenesis of diabetes and its complications. Abelmoschus esculentus (Okra) has been reported to possess many important biological properties. We undertook in vivo studies on male Wistar rats to examine the antioxidative potential of okra in normal and alloxan-treated diabetic rats. Okra extract was administered to control and diabetic rats for 35 consecutive days. Erythrocyte plasma membrane redox system (PMRS) activity (p diabetes for prevention of oxidative stress-mediated complications. PMID:27065051

  3. Selection for Resistance to Yellow Vein Mosaic Virus Disease of Okra by Induced Mutation

    Yellow vein mosaic virus disease (YVMD) caused by a begomovirus is the most serious factor affecting okra (Abelmoschus esculentus) production for both exporting and domestic consumption in Thailand. Seeds of two okra varieties, Annie and Okura, were irradiated with Gamma-rays at doses of 400 and 600Gy. Screening of YVMD resistant plants was conducted for M3 and M4 plants under field conditions in Petchaburi and Phichit provinces, and greenhouse conditions using whitefly transmission in Bangkok. One M4 plant of Okura (B-21) irradiated at 400Gy was found to be highly resistant, but none of Annie. M5 plants of B-21 were screened further for YVMD resistance under both greenhouse and field conditions. Ten resistant lines obtained by screening for YVMD resistance up to the M7 generation were selected for yield trial observations at Phichit Horticultural Research Center (PHRC) and Chiengmai Horticultural Research Station (CHRS), both located in the northern Thailand. Three of the mutant lines were further tested at Kanchanaburi Horticultural Research Center (KHRC) in Kanchanaburi province, an okra growing area in the west of central Thailand where YVMD was seriously widespread. At the KHRC, all tested mutant lines showed resistance up to a month, when the susceptible check variety already showed symptoms of the disease. However, only a small portion of the plants of the mutant lines appeared to be resistant throughout the whole growth duration; others eventually exhibited the yellow vein symptom. Plants were further screened in two growers' fields. Growers were satisfied with the plant stature and fruit shape of the mutants and their delayed disease development, and further screening is underway to select uniformly YVMD resistant lines for okra production in Kanchanaburi. (author)

  4. Film coating potential of okra gum using paracetamol tablets as a model drug

    Ogaji Ikoni; Nnoli Obiageli

    2010-01-01

    The purpose of this work was to study the film coating potential of okra gum extracted from pods of Abelmoschus esculentus plant using paracetamol as a model drug. Core tablets of paracetamol were obtained from a pharmacy shop in the locality and the physicochemical properties such as weight, hardness, friability, and disintegration time were evaluated. Aqueous coating suspensions of okra gum and hydroxypropylmethylcellulose (0.6%w/v) were prepared and used to coat the tablets in Hi-co...

  5. An Overview of Okra Fibre Reinforced Polymer Composites

    Srinivasababu, Nadendla

    2015-05-01

    Increasing attention towards “sustainable environment” invited the development of new materials to satisfy the needs of the public with less/no damage on surroundings. In this regard a specific attempt is made by the author to do review and understand the performance of the variable vegetable fibres of okra which is botanically called ‘Abelmoschus Esculentus’ and their composites under various conditions of load. Further the results exist in the literature are also reviewed for the purpose of comparison.

  6. Genotypic performance, character correlations and path analysis of pod yield in Abelmoschus caillei (A. Chev. Stevels

    Christopher O. Alake

    2012-11-01

    Full Text Available Direct selection based on pod yield is often a problem in West African okra (Abelmoschus caillei (A. Chev. Stevels breeding programs. Information on inter-relationships among traits to implement indirect selection for pod yield is needed. Objectives of this study were to evaluate the performance of West African okra varieties in two cropping seasons prevailing in Nigeria, and to determine the associations among main agronomic characters and their influence on pod yield. Twenty-five genotypes were grown in two planting seasons at the Research Farm of the Federal University of Agriculture, Abeokuta in 2008-2009 using a randomized complete-block design with three replications. Sixteen plant characters were measured. The data were subjected to variance, correlation and path analyses. Four genotypes, viz., CEN 010, CEN 012, NGAE-96-04 and AGA 97/066-5780 demonstrated potential for high pod yield. CEN 012 and AGA 97/066-5780 in addition, demonstrated early flowering higher number of pods. Character associations varied between planting seasons. The dependence of pod yield on final plant height, number of branches per plant, number of pods per branch, number of pods per plant, number of ridges per pod, weight of 250 seeds, pod length and seed weight per plant was noted in both planting seasons. In both seasons, number of pods per plant exhibited a high positive direct effect on pod yield. In conclusion, the trait of most interest for improving pod yield under early and late-season conditions was number of pods per plant.

  7. Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso

    Reynaud Bernard; Traoré Valentin S; Barro Nicolas; Traoré Alfred S; Konaté Gnissa; Villemot Julie; Hoareau Murielle; Lefeuvre Pierre; Tiendrébéogo Fidèle; Traoré Oumar; Lett Jean-Michel

    2010-01-01

    Abstract Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned an...

  8. First report of an alphasatellite associated with Okra enation leaf curl virus.

    Chandran, S A; Packialakshmi, R M; Subhalakshmi, K; Prakash, C; Poovannan, K; Nixon Prabu, A; Gopal, P; Usha, R

    2013-06-01

    An alphasatellite DNA associated with Okra enation leaf curl virus (OELCuV) which causes enation and leaf curling in okra (Abelmoschus esculentus) plants was characterized. The full-length DNA comprises 1,350 nucleotides and shows typical genome organization of an alphasatellite. It shows the highest nucleotide sequence identity (79.7 %) to Hollyhock yellow vein virus-associated symptomless alphasatellite (HoYVSLA). This is the first report of the association of an alphasatellite with OELCuV from India. PMID:23475199

  9. Moisturizing effect of alcohol-based hand rub containing okra polysaccharide.

    Kanlayavattanakul, M; Rodchuea, C; Lourith, N

    2012-06-01

    A natural, moisturizing alcohol-based hand rub (ABHR) containing okra (Abelmoschus esculentus) polysaccharide was formulated to reduce the dryness caused by traditional hand-cleansing products. The ABHR developed also reduced infectious disease transmission. Preliminary evaluations of the stable natural hand hygiene preparations were conducted to determine preference and short-term moisturizing efficacy in volunteers. Formulations contained varying amounts of gelling agent (0.5% and 0.3% w/v). Accelerated stability testing using a centrifugation assay and six heating/cooling cycles of the ABHR bases were performed. Then, okra polysaccharide (5%, 7%, 10% and 15% w/w) was incorporated into the base, and stability tests were repeated. The moisturizing okra polysaccharide was compatible with the formulations at all concentrations. All of the formulated ABHRs were stable. Sensory evaluation was conducted in 36 volunteers. The two most preferred okra ABHRs were patch-tested in 12 volunteers; results indicated none of the preparations caused irritation. Efficacy of the most preferred moisturizing ABHR containing 0.3% gelling agent and 10% (w/v) okra extract was determined. Short-term moisturizing efficacy of a single application was examined in 20 volunteers. The okra ABHR hydrated skin significantly better than a control ABHR (P Skin moisture was retained for 210 min of the observation period. Thus, the ABHR product containing moisturizing okra is safe, efficacious and possesses desirable properties. The formulation can be applied every 3 h for good hand hygiene with moisturizing efficacy. PMID:22404565

  10. Diversity and phylogeography of begomovirus-associated beta satellites of okra in India

    Venkataravanappa V

    2011-12-01

    Full Text Available Abstract Background Okra (Abelmoschus esculentus; family Malvaceae is grown in temperate as well as subtropical regions of the world, both for human consumption as a vegetable and for industrial uses. Okra yields are affected by the diseases caused by phyopathogenic viruses. India is the largest producer of okra and in this region a major biotic constraint to production are viruses of the genus Begomovirus. Begomoviruses affecting okra across the Old World are associated with specific, symptom modulating satellites (beta satellites. We describe a comprehensive analysis of the diversity of beta satellites associated with okra in India. Results The full-length sequences of 36 beta satellites, isolated from okra exhibiting typical begomovirus symptoms (leaf curl and yellow vein, were determined. The sequences segregated in to four groups. Two groups correspond to the beta satellites Okra leaf curl beta satellite (OLCuB and Bhendi yellow vein beta satellite (BYVB that have previously been identified in okra from the sub-continent. One sequence was distinct from all other, previously isolated beta satellites and represents a new species for which we propose the name Bhendi yellow vein India beta satellite (BYVIB. This new beta satellite was nevertheless closely related to BYVB and OLCuB. Most surprising was the identification of Croton yellow vein mosaic beta satellite (CroYVMB in okra; a beta satellite not previously identified in a malvaceous plant species. The okra beta satellites were shown to have distinct geographic host ranges with BYVB occurring across India whereas OLCuB was only identified in northwestern India. Okra infections with CroYVMB were only identified across the northern and eastern central regions of India. A more detailed analysis of the sequences showed that OLCuB, BYVB and BYVIB share highest identity with respect βC1 gene. βC1 is the only gene encoded by beta satellites, the product of which is the major pathogenicity determinant of begomovirus-beta satellite complexes and is involved in overcoming host defenses based on RNAi. Conclusion The diversity of beta satellites in okra across the sub-continent is higher than previously realized and is higher than for any other malvaceous plant species so far analyzed. The beta satellites identified in okra show geographic segregation, which has implications for the development and introduction of resistant okra varieties. However, the finding that the βC1 gene of the major okra beta satellites (OLCuB, BYVB and BYVIB share high sequence identity and provides a possible avenue to achieve a broad spectrum resistance.

  11. Okra : Abelmoschus esculentus, A. caillei, A. manihot, A. moschatus (Malvaceae)

    HAMON, SERGE; Van Sloten, D.H.

    1995-01-01

    Le gombo est un légume-fruit tropical cultivé dans toute la zone intertropicale. Ce texte constitue une des monographies compilées dans la 2ème édition du livre "Evolution des plantes cultivées" de J. Smartt et N.W. Simmonds. Les auteurs S. Hamon (ORSTOM) et D.H. Van Sloten (IPGRI) ont contribué au cours des années 80 à développer des travaux sur cette plante. Dans cet article sont exposées les principales connaissances sur l'évolution de la systématique, les indications taxonomiques, les con...

  12. Complete nucleotide sequences of okra isolates of Cotton leaf curl Gezira virus and their associated DNA-beta from Niger.

    Shih, S L; Kumar, S; Tsai, W S; Lee, L M; Green, S K

    2009-01-01

    Okra (Abelmoschus esculentus) is a major crop in Niger. In the fall of 2007, okra leaf curl disease was observed in Niger and the begomovirus and DNA-beta satellite were found associated with the disease. The complete nucleotide sequences of DNA-A (FJ469626 and FJ469627) and associated DNA-beta satellites (FJ469628 and FJ469629) were determined from two samples. This is the first report of molecular characterization of okra-infecting begomovirus and their associated DNA-beta from Niger. The begomovirus and DNA-beta have been identified as Cotton leaf curl Gezira virus and Cotton leaf curl Gezira betasatellite, respectively, which are reported to also infect okra in Egypt, Mali and Sudan. PMID:19156351

  13. Optimization of microwave-assisted hot air drying conditions of okra using response surface methodology.

    Kumar, Deepak; Prasad, Suresh; Murthy, Ganti S

    2014-02-01

    Okra (Abelmoschus esculentus) was dried to a moisture level of 0.1 g water/g dry matter using a microwave-assisted hot air dryer. Response surface methodology was used to optimize the drying conditions based on specific energy consumption and quality of dried okra. The drying experiments were performed using a central composite rotatable design for three variables: air temperature (40-70 °C), air velocity (1-2 m/s) and microwave power level (0.5-2.5 W/g). The quality of dried okra was determined in terms of color change, rehydration ratio and hardness of texture. A second-order polynomial model was well fitted to all responses and high R(2) values (>0.8) were observed in all cases. The color change of dried okra was found higher at high microwave power and air temperatures. Rehydration properties were better for okra samples dried at higher microwave power levels. Specific energy consumption decreased with increase in microwave power due to decrease in drying time. The drying conditions of 1.51 m/s air velocity, 52.09 °C air temperature and 2.41 W/g microwave power were found optimum for product quality and minimum energy consumption for microwave-convective drying of okra. PMID:24493879

  14. Effect of endophytic pseudomonas aeruinosa and trichoderma harzianum on soil-borne diseases, mycorrhizae and induction of systemic resistance in okra grown in soil amended with vernonia anthelmintica (L.) seeds powder

    Biostimulants are used in agricultural practices for plant growth improvement. These fertilizers improve microbial activity and cause a negative impact on soil-borne pathogens. In recent years, stimulating plant natural defense is considered as most promising alternative strategy for crop productivity. The present study was carried out to examine the effect of endophytic Pseudomonas aeruginosa and Trichoderma harzianum in soil amendment with Vernonia anthelmintica seeds powder, on root rotting fungi, plant growth, mycorrhizal population around roots, phosphorous uptake and stimulation of plant defense markers like poylphenol and antioxidant status in okra. Combine application of Vernonia with Pseudomonas aeruginosa and Trichoderma harzianum significantly (p<0.05) suppressed Rhizoctonia solani and Fusarium oxysporum with complete reduction of Macrophomina phaseolina and Fusarium solani. Pseudomonas aeruginosa and T. harzianum alone or in Vernonia amended soil significantly reduced nematode galls on roots. Organic amendment also improved plant resistance against root diseases as evident from enhanced DPPH radical scavenging capacity and polyphenol content in treated plants as compare to control. VA Mycorrhizal spores were found significantly (p<0.05) higher in number around roots received Pseudomonas aeruginosa or T. harzianum alone or in Vernonia amended soil. Whereas, higher concentrations of phosphorus in okra shoots were found in plants received biocontrol agents in amended soil. Mixed application of PGPR and T. harzianum in amended soil produced tallest plants than other treatments. Soil amendment with Vernonia seed powder alone or with biocontrol agents offer a non-chemical means of plant disease control. (author)

  15. Film coating potential of okra gum using paracetamol tablets as a model drug

    Ogaji Ikoni

    2010-01-01

    Full Text Available The purpose of this work was to study the film coating potential of okra gum extracted from pods of Abelmoschus esculentus plant using paracetamol as a model drug. Core tablets of paracetamol were obtained from a pharmacy shop in the locality and the physicochemical properties such as weight, hardness, friability, and disintegration time were evaluated. Aqueous coating suspensions of okra gum and hydroxypropylmethylcellulose (0.6%w/v were prepared and used to coat the tablets in Hi-coater. The coated tablets were evaluated for weight uniformity, diameter, thickness, hardness, friability, disintegration time, and moisture uptake at controlled humidity. The coating remained intact, durable, and resistant to chipping when challenged to catastrophic fall or rubbed on a white paper. The coated tablets had lower friability, increased disintegration time (24 min compared to the core (3 min and improved hardness, but there was no difference in the dissolution profile of the samples from the batches containing okra and hydroxypropylmethylcellulose as film formers. Changes were observed in some of the physicochemical properties of the formulations containing okra gum as with the known film former and it was convenient to conclude that these changes were due to the effect of the mechanical properties of the film formers. It was our conclusion that okra gum is a promising natural, biodegradable, cheap and eco-friendly film former in aqueous tablet film coating operation, particularly when masking of taste or objectionable odor in a solid dosage formulation is desired.

  16. Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa.

    Kon, Tatsuya; Rojas, Maria R; Abdourhamane, Issoufou K; Gilbertson, Robert L

    2009-04-01

    Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production in West Africa. Two monopartite begomoviruses (okra virus-1 and okra virus-2), a betasatellite and a DNA1 satellite are associated with OLCD in Mali. Okra virus-1 is an isolate of okra yellow crinkle virus (OYCrV), okra virus-2 is a recombinant isolate of cotton leaf curl Gezira virus (CLCuGV) and the betasatellite is a variant of cotton leaf curl Gezira betasatellite (CLCuGB). Cloned DNA of OYCrV and CLCuGV were infectious and induced leaf curl symptoms in Nicotiana benthamiana plants, but did not induce OLCD in okra. However, when these clones were individually co-inoculated with the cloned CLCuGB DNA, symptom severity and viral DNA levels were increased in N. benthamiana plants and typical OLCD symptoms were induced in okra. The CLCuGB was also replicated by, and increased symptom severity of, three monopartite tomato-infecting begomoviruses, including two from West Africa. The sequence of the DNA1 satellite was highly divergent, indicating that it represents a distinct West African lineage. DNA1 replicated autonomously, and replication required the DNA1-encoded Rep protein. Although DNA1 reduced helper begomovirus DNA levels, symptoms were not attenuated. In the presence of CLCuGB, DNA levels of the helper begomoviruses and DNA1 were substantially increased. Together, these findings establish that OLCD in Mali is caused by a complex of monopartite begomoviruses and a promiscuous betasatellite with an associated parasitic DNA1 satellite. These findings are discussed in terms of the aetiology of OLCD and the evolution of new begomovirus/satellite DNA complexes. PMID:19264648

  17. Summer cover crops and soil amendments to improve growth and nutrient uptake of okra

    Wang, Q.R.; Li, Y.C.; Klassen, W. [University of Florida, Homestead, FL (United States). Center for Tropical Research & Education

    2006-04-15

    A pot experiment with summer cover crops and soil amendments was conducted in two consecutive years to elucidate the effects of these cover crops and soil amendments on 'Clemson Spineless 80' okra (Abelmoschus esculentus) yields and biomass production, and the uptake and distribution of soil nutrients and trace elements. The cover crops were sunn hemp (Crotalaria juncea), cowpea (Vigna unguiculata), velvetbean (Mucuna deeringiana), and sorghum sudan-grass (Sorghum bicolor x S. bicolor var. sudanense) with fallow as the control. The organic soil amendments were biosolids (sediment from wastewater plants), N-Viro Soil (a mixture of biosolids and coal ash), coal ash (a combustion by-product from power plants), co-compost (a mixture of 3 biosolids: 7 yard waste), and yard waste compost (mainly from leaves and branches of trees and shrubs, and grass clippings) with a soil-incorporated cover crop as the control. As a subsequent vegetable crop, okra was grown after the cover crops, alone or together with the organic soil amendments, had been incorporated. All of the cover crops, except sorghum sudangrass in 2002-03, significantly improved okra fruit yields and the total biomass production. Both cover crops and soil amendments can substantially improve nutrient uptake and distribution. The results suggest that cover crops and appropriate amounts of soil amendments can be used to improve soil fertility and okra yield without adverse environmental effects or risk of contamination of the fruit. Further field studies will be required to confirm these findings.

  18. Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelmoschus esculentus L.) in India.

    Venkataravanappa, V; Lakshminarayana Reddy, C N; Jalali, Salil; Krishna Reddy, M

    2012-06-01

    Yellow vein mosaic disease of okra is a whitefly transmitted begomovirus causing heavy economic loss in different parts of India. The okra isolate (OY131) of this virus from a bhendi plant [(Abelmoschus esculentus L.) Moench] showing yellow vein mosaic, vein twisting, reduced leaves, and a bushy appearance in the Palem region, New Delhi, India, was characterized in the present study. The complete DNA-A and DNA-B sequences have been determined and are comprised of 2,746 and 2,703 nucleotides, respectively. The betasatellite (DNA-?) component was absent in the sample. The genome organization was typically of biparite begomoviruses, which were characterized earlier. Comparison of DNA-A component with other known begomoviruses suggest that this virus, being only distantly related (okra)]. DNA-B showed highest sequence identity (87.8% identical) to that of a ToLCNDV (AY158080). The phylogenetic analysis of the present isolate is distinct from all other viruses; however clusters with ToLCNDV group infect different crops. The recombination analysis revealed that this isolate has sequences originated from ToLCNDV. This is the first known bhendi yellow vein mosaic disease associated bipartite begomovirus from India. PMID:22447131

  19. Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India.

    Venkataravanappa, V; Lakshminarayana Reddy, C N; Devaraju, A; Jalali, Salil; Krishna Reddy, M

    2013-09-01

    A begomovirus isolate (OY136A) collected from okra plants showing upward leaf curling, vein clearing, vein thickening and yellowing symptoms from Bangalore rural district, Karnataka, India was characterized. The sequence comparisons revealed that, this virus isolate share highest nucleotide identity with isolates of Cotton leaf curl Bangalore virus (CLCuBV) (AY705380) (92.8 %) and Okra enation leaf curl virus (81.1-86.2 %). This is well supported by phylogentic analysis showing, close clustering of the virus isolate with CLCuBV. With this data, based on the current taxonomic criteria for the genus Begomovirus, the present virus isolate is classified as a new strain of CLCuBV, for which CLCuBV-[India: Bangalore: okra: 2006] additional descriptor is proposed. The betasatellite (KC608158) associated with the virus is having more than 95 % sequence similarity with the cotton leaf curl betasatellites (CLCuB) available in the GenBank.The recombination analysis suggested, emergence of this new strain of okra infecting begomovirus might have been from the exchange of genetic material between BYVMV and CLCuMuV. The virus was successfully transmitted by whitefly and grafting. The host range of the virus was shown to be very narrow and limited to two species in the family Malvaceae, okra (Abelmoschus esculentus) and hollyhock (Althaea rosea), and four in the family Solanaceae. PMID:24426275

  20. EFFECT OF DIFFERENT INOCULATION METHODS AND INOCULUM LEVELS OF MACROPHOMINA PHASEOLINA ON OKRA

    Sultan A. Maitlo

    2012-12-01

    Full Text Available Among two methods of Macrophomina phaseolina inoculation used for pathogenicity test, soil infestation method comparatively checked more plant growth of okra plants than seed infestation method. Minimum plant length and weight, as well as seed germination were observed by soil infestation method. Significantly maximum plant mortality and root infection was also occurred in soil infestation method. Seed germination, plant growth, plant mortality and root infection of okra plants were adversely affected with the increasing inoculum levels of M. phaseolina. Seed germination and plant growth were negatively correlated with inoculated pathogen population; whereas, plant mortality and root infection were positively correlated with the inoculum level of M. phaseolina.

  1. Expression de différents écotypes de gombo (Abelmoschus esculentus L. au déficit hydrique intervenant pendant la boutonnisation et la floraison

    Sawadogo M., Zombre G., Balma D.

    2006-01-01

    Full Text Available Behaviour of different ecotypes of okra (Abelmoschus esculentus L. under water deficit during budding and flowering phases. Despite numerous uses and its well known nutritional value, okra is cultivated on very small surfaces, on surroundings of traditional houses and often in dumping ground. To this little interest are added drought, diseases and pests that generate an important reduction of yield, leading okra to play the last roles in agricultural research programs of the country and this, despite the existence of early maturing ecotypes or resistant to some abiotic constraints. The low rate of improved varieties is the proof that it is necessary to develop strategies for participatory breeding programs that take into account farmers selection criteria including genotypes tolerant or resistant to drastic conditions such as water deficit to periods where the plant is expressing its genetic potential. Six ecotypes obtained through participatory plant breeding process in five agricultural districts of the country have been assessed for the tolerance to the drought occurring during the budding and the flowering phases. Results show that water deficit during budding phase has disastrous consequences for okra plants. The yield declines, and also the length, the number and the weight of fruits. Two types of period of flowering phases of okra plant have been observed: the period which intervenes during the accelerated growth phase of the main stem (type 1 and the one which starts once the growth of main stem begins to decline (type 2; this last type is the most frequently met. Water deficit during the phase of budding causes earlier flowering of type 2 plants and leads to delayed flowering of the type 1 plants. The best variables for discriminating okra ecotypes during water deficit are the relative water content (RWC, the length and the number of fruits. Three ecotypes (V1, V3 and V4 are chosen to be used in breeding program of okra for resistance to water deficit.

  2. PRODUCTIVITY OF OKRA VARIETIES AS INFLUENCED BY SEASONAL CHANGES IN NORTHERN NIGERIA

    M. D. KATUNG

    2007-08-01

    Full Text Available In northern Nigeria, two distinct season are noticeable and these are the wet (April to October and the dry (November to March seasons. Due to little or no documented work conducted to investigate the influence of climatic changes on the performance of okra (Abelmoschus esculentus (L. Moench, this study is therefore undertaken to compare the performance of okra during the wet and the dry seasons of northern Nigeria. Two okra varieties ('White velvet' and 'Ex-Borno' were evaluated during the 1997 and 1999 wet seasons and 1997/98 and 1999/2000 dry seasons at Samaru in northern Nigeria, in order to determine their productivity. The variables measured at the reproductive stage were plant height, number of leaves/plant, leaf area, days to first flower, fruit weight/plant and fruit yield (t/ha. Significant differences were observed for all variables among seasons and among varieties. The wet season conditions were most favourable for increased growth, leaf formation and fruit yield, as compared with the dry season environment which resulted in less vegetative and reproductive growth. The interaction of season x variety was highly significant for plant height, fruit weight/plant, fruit yield and significant for leaf area and number of fruits/plant. The variety 'White velvet' produced more fruits than 'Ex-Borno'.

  3. Heavy metals phyto-assessment in commonly grown vegetables: water spinach (I. aquatica) and okra (A. esculentus).

    Ng, Chuck Chuan; Rahman, Md Motior; Boyce, Amru Nasrulhaq; Abas, Mhd Radzi

    2016-01-01

    The growth response, metal tolerance and phytoaccumulation properties of water spinach (Ipomoea aquatica) and okra (Abelmoschus esculentus) were assessed under different contaminated spiked metals: control, 50 mg Pb/kg soil, 50 mg Zn/kg soil and 50 mg Cu/kg soil. The availability of Pb, Zn and Cu metals in both soil and plants were detected using flame atomic absorption spectrometry. The concentration and accumulation of heavy metals from soil to roots and shoots (edible parts) were evaluated in terms of translocation factor, accumulation factor and tolerance index. Okra recorded the highest accumulation of Pb (80.20 mg/kg) in its root followed by Zn in roots (35.70 mg/kg) and shoots (34.80 mg/kg) of water spinach, respectively. Different accumulation trends were observed with, Pb > Zn > Cu in okra and Zn > Pb > Cu in water spinach. Significant differences (p Malaysian Food Act 1983 and Food Regulations 1985 (2006) as well as the international Codex Alimentarius Commission limits. This study has shown that both water spinach and okra have good potential as Pb and Zn phytoremediators. PMID:27119073

  4. Effects of different soil amendments on the growth and yield of okra in a tropical rainforest of southwestern Nigeria

    Adewole Moses B.

    2012-01-01

    Full Text Available This study investigated the effects of different soil enhancers on the growth response of okra [Abelmoschus esculentus (L. Moench] cultivated on a ‘contaminated’ field with sewage sludge from the two oxidation ponds of the Obafemi Awolowo University (OAU, Ile-Ife, Nigeria. This was with a view to assessing the growth performance and yield of the test crop under different soil amendments. Okra variety, NHAe 47-4 with NPK 12-12-17 (IO, compost organic fertilizer (OR, Glomus mosseae mycorrhiza (MY and zero fertilizer applications as control (CT was laid out in a completely randomised block design and each treatment plot (4 x 2 m was replicated four times. Selected weather parameters were collected from a meteorological station in OAU campus during the period of the experiments. Growth parameters such as plant height, stem girth and number of leaves of okra increased with added soil amendments from four weeks after planting in the order: IO > OR > MY > CT. In 2010, the highest mean yield of 16.3 t ha-1 obtained with 6.0 t ha-1 of MY was not significantly higher than 15.4 t ha-1 obtained with application of 0.2 t ha-1 of IO, but significantly (p < 0.05 higher than 13.1 and 10.4 t ha-1 obtained with applications of 6.0 and zero t ha-1 of OR and CT respectively. Comparative okra yield, though relatively higher with mycorrhizal inoculation, but lower with no soil amendment was obtained in 2011. The study concluded that a direct linear relationship existed between solar radiation and okra productivity. Also, for a moderately ‘treated field’ with sewage sludge from domestic wastes, arbuscular mycorrhizal fungi can be integrated into soil fertility management to achieve low-cost sustainable agricultural systems for enhanced productivity of okra.

  5. MORPHOLOGICAL, ANATOMICAL, CYTOLOGICAL AND PALYNOLOGICAL CHARACTERIZATION OF TWO CULTIVARS OF ABELMOSCHUS MOSCHATUS (L.) MEDIK (MALVACEAE)

    Dubey Kumari Priyanka; Datta K. Animesh; Mandal Aninda; Saha Aditi; Sengupta Sonali

    2011-01-01

    Seeds of 2 cultivars (cultivars I: seed moisture content 3.5%, 100 seed weight 1.94 gm ± 0.03, seed size 3.47 mm ± 0.08 × 3.20 mm ± 0.06; cultivars II: seed moisture content 1.5%, 100 seed weight 1.25 gm ± 0.01, seed size 2.92 mm ± 0.07 × 3.33 mm ± 0.03) of Abelmoschus moschatus (L.) Medik (Family: Malvaceae) were grown in the Experimental plots of University of Kalyani (West Bengal plain; 22°99´ N, 88°45´ E, elevation 48 feet above sea level, sandy loamy soil, organic carbon 0.76%, soil pH 6...

  6. Screening of dried plant seed extracts for adiponectin production activity and tumor necrosis factor-alpha inhibitory activity on 3T3-L1 adipocytes.

    Okada, Yoshinori; Okada, Mizue; Sagesaka, Yumi

    2010-09-01

    To search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. These activities in 3T3-L1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in Japan. komatsuna (Brassica rapa var. perviridis), common bean (Phaseolus vulgaris L.), qing geng cai (Brassica rapa var. chinensis), green soybean (Glycine max), spinach (Spinacia oleracea L.) and sugar snap pea (Pisum sativum L.) markedly enhanced adiponectin production (11.3 ~ 12.7 ng/ml) but Japanese radish (Raphanus sativus), edible burdock (Arctium lappa L.), bitter melon (Momordica charantia) and broccoli (Brassica oleracea var. italica) did not (0.9 ~ 2.7 ng/ml). All adiponectin-production-enhancing seeds except spinach (2.7 pg/ml) and okra (Abelmoschus esculentus) (6.6 pg/ml) effectively decreased tumor necrosis factor-alpha levels (0.0 pg/ml). We further examined the effects on free radical scavenging activities in the dried seed extracts. Although scavenging activity correlated well with total phenolic content of samples, no correlation was observed with adiponectin production. These results point to the potential of dried seed extracts as a means to modify the activity of tumor necrosis factor-alpha for the adiponectin production. PMID:20717728

  7. Search for sources of resistance to Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum in okra germplasm

    Frederick Mendes Aguiar

    2013-04-01

    Full Text Available – Fusarium oxysporum f. sp. vasinfectum (FOV is one the most destructive okra (Abelmoschus esculentus pathogens in Brazil. Fifty-four okra accessions were evaluated for resistance to FOV. Greenhouse screening was initially carried out with one FOV isolate (‘Fus-194’. Inoculation (in all assays was carried out with 21-day-old plantlets, using the root-dipping inoculation technique. Thirty-three accessions displaying differential responses in the first screening were re-evaluated in two additional assays, using two FOV isolates (‘Fus-194’ and ‘Fus-201’. Twelve accessions were rated as highly to intermediately resistant to ‘Fus-194’ during the dry/moderate temperature season, whereas nine accessions were classified as highly to intermediately resistant to ‘Fus-201’. In the assay carried out in the wet and warm season, 72% of the accessions were classified as having high and intermediate resistance to ‘Fus-194’, and 32% were resistant to ‘Fus-201’. The accessions ‘Santa Cruz-47’, ‘BR-2399’ and ‘BR-1449’ were the most promising resistance sources.

  8. Impact Of Chlorpyriphos On The Morphological Parameters Of Cauliflower, Tomato And Okra

    Mosmi Raina

    2012-09-01

    Full Text Available The present study has been conducted to work out the effect of a wide spectrum organophosphorous insecticide, chlorpyriphos (O, O-Diethyl O- 3, 5, 6-trichloro-2-pyridyl phosphorothioate on the morphological features of three commonly grown vegetables in the study area i.e. Cauliflower (Brassica oleracea L. var. botrytis, Variety: Snowball 16, Tomato (Lycopersicon esculentum Mill., Variety: Pusa Ruby and Okra (Abelmoschus esculentus L., Variety: Pusa Sawani. Results revealed that on an average, the impact of chlorpyriphos on various morphological parameters of all the three vegetables have been statistically insignificant (using t- Test except for height of the plants in cauliflower treated with double the recommended dose which exhibited a significant decrease and weight of the head in cauliflower treated with recommended dose of chlorpyriphos which showed a significant increase. Also in okra total number of flowers/plant at treatment with both the dosages along with total number of fruits/plant at treatment with the recommended dose has exhibited statistically significant increase.

  9. Single and interactive effects of root-knot nematode and coal-smoke on okra

    Khan, M.R.; Khan, M.W. (Aligarh Muslim University, Aligarh (India). Dept. of Botany)

    1994-02-01

    Effects of coal-smoke pollutants and the root-knot nematode Meloidogyne incognita race 1, singly and jointly, were investigated on okra, Abelmoschus esculentus in 1988 and 1989. Plants in clay pots were placed at two sites (K1 and K2), 1 and 2 km away from the stack of a coal-fired thermal power plant, and also at a control site. The mean concentrations of SO[sub 2], NO[sub 2] and suspended particulate matter (SPM) were 141, 76 and 309 [mu]g m[sup -3] at K1, and 184, 93 and 205 [mu]g m[sup -3] at K2, but the concentrations of the pollutants at the control site were very low. Okra plants at the K2 site showed browning of the leaves, whereas injury was mild at K1. The intensity of the browning and the impact of nematode disease was appreciably higher in the infected plants at the polluted sites in both the years. The plants grown at the two sites exhibited suppressions in plant growth, yield and photosynthetic pigments, particularly at K2. M. incognita at the control site also cause significant reductions, but joint effects of the nematode and coal-smoke were synergistic.

  10. Seed priming with extracts of Acacia nilotica (L.) Willd. ex Delile and Sapindus mukorossi (L.) plant parts in the control of root rot fungi and growth of plants

    Seed priming with plant extracts and chemicals has been used as an important growth enhancement tool in crop plants. In this research, an attempt was made to understand the mechanism of various seed priming treatments on greenhouse-grown okra (Abelmoschus esculentus (L.) Moench.), sunflower (Helianthus annuus L.), peanut (Arachis hypogaea L.) and chickpea (Cicer arietinum L.) for the control of root infecting fungi like Rhizoctonia solani (Kn), Fusarium spp. and Macrophomina phaseolina (Tassi) Goid by plant parts extracts (stem, leaves and seeds) of Acacia nilotica (L.) Willd. ex Delile and Sapindus mukorossi (L) at different time intervals (5, 10, 20, 40 minutes). Results showed significant suppression of root rot fungi and significantly enhanced the growth parameters like shoot length, root length, shoot weight and root weight. Seed-priming with A. nilotica and S. mukorossi leaves extract for 10 minutes time interval was found to be effective for the control of root rot fungi and growth of all tested leguminous and non-leguminous plants. (author)

  11. Agro-potentiality of distillery effluent on soil and agronomical characteristics of Abelmoschus esculentus L. (okra).

    Chopra, A K; Srivastava, Sachin; Kumar, Vinod; Pathak, Chakresh

    2013-08-01

    The present study showed that irrigation of soil with different effluent concentrations (10, 25, 50, 75, and 100 %) of distillery effluent (DE) for 60 days resulted in significant (P??0.05) changes were observed for water-holding capacity and bulk density of the soil. Among various concentrations of DE irrigation, irrigation with 100 % effluent concentration increased moisture content, (24.85 %), EC (77.88 %), Cl(-) (285.95 %), TOC (3,171.42 %), exchangeable Na(+) (241.04 %), available K(+) (52.49 %), Ca(2+) (990.37 %), Mg(2+) (1,751.72 %), TKN (1,417.00 %), available P (305.00 %), and SO4 (2-) (75.32 %) in the soil and decreased pH (-20.22 %). The more stimulation in agronomical parameters such as shoot length, root length, number of leaves, flowers, pods, dry weight, fresh weight, chlorophyll content, leaf area index, and crop yield of A. esculentus were observed to be inversely proportional to the concentration of effluent water, with the best results being obtained at a dilution of 25 % of DE concentration. PMID:23264061

  12. Combining ability studies in okra (abelmoschus esculentus (l. moench for yield and its component characters

    J.P. Bhatt, K.B. Kathiria, S. S. Christian and R. R. Acharya

    2015-06-01

    Full Text Available The present experiment was conducted with 28 F1s developed through diallel hybridization technique excluding reciprocals along with eight parents in RBD with three replications. The gca and sca mean squares were significant for all the traits except gca mean square for fruit length. The ratio of gca and sca variances indicated the preponderance of non-additive gene effect for inheritance of all the traits. Genotypes AOL-09-25 and GO-2 showed good general combining ability for fruit yield appear to be worthy for exploitation of segregation and varietal development. The estimates of sca effects revealed that the cross combinations AOL-09-25 x AOL-09-26, GO-2 x AOL-09-28 and AOL-08-10 x AOL-08-2 were observed most promising for fruit yield and some of its related traits.

  13. Preventative and Curative Effects of Several Plant Derived Agents Against Powdery Mildew Disease of Okra

    Moustafa Hemdan Ahmed MOHARAM; Hazim Abd El-Rahman OBIADALLA ALI

    2012-01-01

    The preventative and curative effects of some plant derived agents based on plant extracts or essential oils were studied at different concentrations against Erysiphe cichoracearum DC. ex Merat, the causal pathogen of okra powdery mildew by the detached leaf-disk and potted plants bioassays. Through detached leaf-disk assay, the highest mean preventative effect (97.74%) was recorded by neem seed oil followed by jojoba oil (89.82%) and extract of Rynoutria sachalinensis (82.77%). Neem seed oil...

  14. Effect of electromagnetic field on okra (Hibiscus sculentus L.) developmental stages and the effect of okra extract on breast cancer cells

    Z. Hasani Sadrabadi; A.Majd; M. Hamzeloo-Moghdam; S. Irian

    2016-01-01

    Background and objectives: Electric, magnetic and electromagnetic fields can act as stress factors with different effects on biological systems. Due to the nutritional and medicinal values, and the increasing electromagnetic radiations, the present study was performed to investigate the effects of the electromagnetic field on the developmental stages and cytotoxic properties of okra. Methods: Both dry and wet seeds were exposed to electromagnetic field with the intensities of 2 and 4 mT for 6...

  15. Effect of electromagnetic field on okra (Hibiscus sculentus L. developmental stages and the effect of okra extract on breast cancer cells

    Z. Hasani Sadrabadi

    2016-04-01

    Full Text Available Background and objectives: Electric, magnetic and electromagnetic fields can act as stress factors with different effects on biological systems. Due to the nutritional and medicinal values, and the increasing electromagnetic radiations, the present study was performed to investigate the effects of the electromagnetic field on the developmental stages and cytotoxic properties of okra. Methods: Both dry and wet seeds were exposed to electromagnetic field with the intensities of 2 and 4 mT for 60 minutes. MTT assay was applied to evaluate the potential cytotoxic effects of okra extract on MCF-7 cell line. The anatomical structure of leaves in both treated and untreated (control plants were examined. Results: The results showed that a field intensity of 4 mT increased the speed of germination of wet treated seeds and the stem length of dry treated seeds. MTT assay revealed no cytotoxicity of the aqueous extracts of okra pods up to the concentration of 100 μg/mL from either the treated or the control plants towards the MCF-7 cell line. Conclusion: The results suggest that the electromagnetic fields would be able to increase the speed of germination without effects on percentage of germination.

  16. Purification, characterization and immunomodulating activity of a polysaccharide from flowers of Abelmoschus esculentus.

    Zheng, Wei; Zhao, Ting; Feng, Weiwei; Wang, Wei; Zou, Ye; Zheng, Daheng; Takase, Mohammed; Li, Qian; Wu, Huiyu; Yang, Liuqing; Wu, Xiangyang

    2014-06-15

    A water-soluble polysaccharide (OFPS11) was obtained from okra (Abelmoschus esculentus) flowers using aqueous extraction and purification with DEAE-52 cellulose and Sephacryl™ S-500 column. Its preliminary characterization and immunomodulating activity were investigated. Results showed that OFPS11 is mainly composed of galactose and rhamnose in a molar ratio of 2.23:1 with molecular mass of 1,700 kDa. RAW264.7 cells pretreated with OFPS11 significantly inhibited the proliferation of HepG-2 cells. Additionally, OFPS11 enhanced the phagocytic ability and induced the elevation of NO production, TNF-? and IL-1? secretion of RAW264.7 cells. Furthermore, OFPS11 promoted both the expression of iNOS protein and of iNOS and TNF-? mRNA. OFPS11 can strongly increase NF-?B levels in nucleuses, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-?. These outcomes support that OFPS11 exerts its antitumor activity by probably stimulating macrophage activities through nuclear NF-?B pathway. PMID:24721087

  17. Combining ability in bhindi [ Abelmoschus spp.

    Divya Balakrishnan

    2009-12-01

    Full Text Available Combining ability was estimated for six diverse okra genotypes or varieties by diallel analysis. The combininganalysis revealed that Arka Anamika was found to be a good general combiner for fruit number, fruit weight and fruit length.KL9 showed high gca for days to first flowering, internode number and fruit weight. In this study, it was observed thatpresence of overdominance for most of the yield contributing traits

  18. Tensile Properties Characterization of Okra Woven Fiber Reinforced Polyester Composites

    Srinivasababu; K. Murali Mohan Rao; J.Suresh Kumar

    2009-01-01

    The present research exploits a new natural fiber namely okra for the preparationof okra fiber reinforced polyester composites. Chemically treated (chemicaltreatment-2) okra woven FRP composites showed the highest tensile strengthand modulus of 64.41 MPa and 946.44 MPa respectively than all othercomposites investigated in the present research. Specific tensile strength andmodulus of untreated and treated okra FRP composites is 34.31% and 39.84%higher than pure polyester specimen respectively.

  19. Tensile Properties Characterization of Okra Woven Fiber Reinforced Polyester Composites

    Srinivasababu

    2009-10-01

    Full Text Available The present research exploits a new natural fiber namely okra for the preparationof okra fiber reinforced polyester composites. Chemically treated (chemicaltreatment-2 okra woven FRP composites showed the highest tensile strengthand modulus of 64.41 MPa and 946.44 MPa respectively than all othercomposites investigated in the present research. Specific tensile strength andmodulus of untreated and treated okra FRP composites is 34.31% and 39.84%higher than pure polyester specimen respectively.

  20. Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso.

    Tiendrébéogo, Fidèle; Lefeuvre, Pierre; Hoareau, Murielle; Villemot, Julie; Konaté, Gnissa; Traoré, Alfred S; Barro, Nicolas; Traoré, Valentin S; Reynaud, Bernard; Traoré, Oumar; Lett, Jean-Michel

    2010-01-01

    Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD. PMID:20178575

  1. Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso

    Reynaud Bernard

    2010-02-01

    Full Text Available Abstract Okra leaf curl disease (OLCD is a major constraint on okra (Abelmoschus esculentus production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF, we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1 molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB (88 to 98% nucleotide identity. One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA (89% nucleotide identity, whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity, provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA. Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009 and Tomato leaf curl Diana virus (AM701765. Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD.

  2. Global ex-situ crop diversity conservation and the Svalbard Global Seed Vault: assessing the current status.

    Westengen, Ola T; Jeppson, Simon; Guarino, Luigi

    2013-01-01

    Ex-situ conservation of crop diversity is a global concern, and the development of an efficient and sustainable conservation system is a historic priority recognized in international law and policy. We assess the completeness of the safety duplication collection in the Svalbard Global Seed Vault with respect to data on the world's ex-situ collections as reported by the Food and Agriculture Organization of the United Nations. Currently, 774,601 samples are deposited at Svalbard by 53 genebanks. We estimate that more than one third of the globally distinct accessions of 156 crop genera stored in genebanks as orthodox seeds are conserved in the Seed Vault. The numbers of safety duplicates of Triticum (wheat), Sorghum (sorghum), Pennisetum (pearl millet), Eleusine (finger millet), Cicer (chickpea) and Lens (lentil) exceed 50% of the estimated numbers of distinct accessions in global ex-situ collections. The number of accessions conserved globally generally reflects importance for food production, but there are significant gaps in the safety collection at Svalbard in some genera of high importance for food security in tropical countries, such as Amaranthus (amaranth), Chenopodium (quinoa), Eragrostis (teff) and Abelmoschus (okra). In the 29 food-crop genera with the largest number of accessions stored globally, an average of 5.5 out of the ten largest collections is already represented in the Seed Vault collection or is covered by existing deposit agreements. The high coverage of ITPGRFA Annex 1 crops and of those crops for which there is a CGIAR mandate in the current Seed Vault collection indicates that existence of international policies and institutions are important determinants for accessions to be safety duplicated at Svalbard. As a back-up site for the global conservation system, the Seed Vault plays not only a practical but also a symbolic role for enhanced integration and cooperation for conservation of crop diversity. PMID:23671707

  3. A Landmark Approach to Aphrodisiac Property of Abelmoschus manihot (L.

    K.K. Rewatkar

    2011-04-01

    Full Text Available

    The Abelmoschus manihot (L. commonly reffered to as “Junglee bhindi” is widely used to control fertility, depression and anxiety in traditional Chinese medicine and has potential therapeutic benefit for cardiovascular diseases associated with diabetes mellitus. The present study is aimed to investigate the effect of 95% ethanolic extract of Abelmoschus manihot on general mounting frequency, intromission frequency, penile erection index along with body weight/organ weight and sperm count on sexually normal male mice. Two doses i.e. 100and 200 mg/kg b.w. of ethanolic extract administered to Swiss albino mice, showed pronounced anabolic and spermatogenic effect in animals of respective groups. There was a remarkable increased in sperm count and penile erection index and also improved sexual behavior of male mice by increased mount and intromission frequency.The result of the present study signatured for sexual enhancing capacity of the drug Abelmoschus manihot is an individual and also holds good aphrodisiac property when compared with standard drug. It was noticed that a 200 mg/kg b.w. dose of Abelmoschus manihot, the performance rate enhances without any side effect. Therefore, the conclusion suggestive that the Abelmoschus manihot will be a drug of choice or alternative therapy for a marketed product. Which may help the population to lead their sexual life perfectly with full of pleasure to interact body, mind and sole.

    Keywords: Abelmoschus manihot, Aphrodisiac, Mounting frequency, Intromission frequency, Penile erection index.

  4. FORMULATION AND EVALUATION OF MATRIX TABLETS BASED ON POLYELECTROLYTE COMPLEX BETWEEN OKRA MUCILAGE AND CHITOSAN

    Ashwini Rajendra

    2012-02-01

    Full Text Available Recent years there has been greater utilization of natural polymers in the development of delivery systems. The present work is an effort towards development of matrix tablets using polyelectrolyte complex formed between the oppositely charged natural polymers like okra mucilage obtained from pods of Abelmoschus esculentus and chitosan. The effect of pH and polymer volume ratio on yield of polyelectrolyte complex was studied. It was observed that the yield was maximum (96.45% at pH 5 and at polymer volume ratio of 9:1 between okra mucilage and chitosan. The prepared polyelectrolyte complex was also characterised by conductimetry, FTIR, DSC. The results confirmed the formation of polyelectrolyte complex between the natural polymers. The matrix tablets were formulated for model drug diclofenac sodium using the best polyelectrolyte complex at different drug to polymer ratios and compared with formulations containing individual polymers as well as marketed formulation. The prepared formulations showed satisfactory physical parameters. Formulations F2 and F3 extended the drug release for more than 8 h with (83.87± 0.8321% and (77.125± 0.125% drug release respectively in 8 h. The formulations F2 and F3 followed zero order kinetics with anomalous diffusion mechanism. The mean dissolution times were 3.6042 and 3.5935 hrs and the % dissolution efficiency were 54.9467 and 55.7203 % for formulations F2 and F3 respectively. The similarity factor f2 for formulation F2 was 61.6751 and for formulation F3, it was found to be 60.5025.The formulations were found to be stable.

  5. MORPHOLOGICAL, ANATOMICAL, CYTOLOGICAL AND PALYNOLOGICAL CHARACTERIZATION OF TWO CULTIVARS OF ABELMOSCHUS MOSCHATUS (L. MEDIK (MALVACEAE

    Dubey Kumari Priyanka

    2011-02-01

    Full Text Available Seeds of 2 cultivars (cultivars I: seed moisture content 3.5%, 100 seed weight 1.94 gm ± 0.03, seed size 3.47 mm ± 0.08 × 3.20 mm ± 0.06; cultivars II: seed moisture content 1.5%, 100 seed weight 1.25 gm ± 0.01, seed size 2.92 mm ± 0.07 × 3.33 mm ± 0.03 of Abelmoschus moschatus (L. Medik (Family: Malvaceae were grown in the Experimental plots of University of Kalyani (West Bengal plain; 22°99´ N, 88°45´ E, elevation 48 feet above sea level, sandy loamy soil, organic carbon 0.76%, soil pH 6.85 during the rainfed seasons of 2009 and 2010 (July to December and morphological (taxonomical details, anatomical (transverse sections of ovary, stem and root, stomatal, cytological (meiotic chromosome behavior and pollen attributes (fertility, viability and acetolysis studies were assessed. Results indicated marked differences between the cultivars and based on observations cultivar I is recommended as tall, branched whereas cultivar II as dwarf, unbranched types and the cultivars may further be explored for pharmacological research.

  6. Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.

    Thöle, Christian; Brandt, Simone; Ahmed, Niyaz; Hensel, Andreas

    2015-01-01

    Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue. The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells. Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL. Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates. Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x). A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%. Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains. Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity. PMID:26389872

  7. Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins

    Christian Thöle

    2015-09-01

    Full Text Available Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori to stomach tissue. The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells. Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates after preincubation of the bacteria at 1 mg/mL. Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS with dose dependent antiadhesive effects against strain J99 and three clinical isolates. Preincubation of H. pylori with HBSS (1 mg/mL led to reduced binding to 3ʹ-sialyl lactose, sialylated Lea and Lex. A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%. Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains. Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.

  8. Selection for resistance to yellow vein mosaic virus disease of okra by induced mutation

    Yellow vein mosaic virus disease (YVMD) caused by a begomovirus is the most serious factor affecting okra (Abelmochus esculentus) production for both export and domestic consumption in Thailand. Seeds of Annie and Okura okra varieties were gamma-irradiated at doses of 400 and 600 Gy and planted at Huaysai King's Project in Petchaburi Province. M3 plants were screened for OYVMD (Okra YVMD) resistance under field conditions at Huaysai King's Project and Phichit Horticultural Research Center (PHRC) in Phichit Province. In addition, M4 plants were screened for OYVMD resistance under greenhouse conditions at Crop Protection Research and Development Office using whitefly transmission. None of Annie was found resistant but one plant of Okura (B-21) irradiated at 400 Gy was found to be highly resistant. Ten resistant lines obtained through rescreening of B-21 descendants up to M7 generation were selected for yield trial observations at PHRC and Chiengmai Horticultural Research Station (CHRS). The mutants had good stature and fruit shape but the fruits have spines on the ridges. Selections for OYVMD resistance and spineless fruits were performed at PHRC in three generations and seven of the lines were chosen for yield trial at PHRC. Three of the mutant lines were also screened for OYVMD resistance at Kanchanaburi Horticultural Research Center (KHRC) in Kanchanaburi Province, okra growing area, where OYVMD was seriously widespread. All mutant lines showed resistance against the local OYVMV isolates up to a month before they started showing signs of the disease. Seeds were collected from resistant individuals and planted in farmers's fields for further selection. The farmers were very satisfied with the stature and fruit shape of the mutants when tested against a commercial variety. (author)

  9. Okra Leaf Cotton, its Commercial Utilization in Sindh

    A.R. Soomro

    2000-01-01

    Full Text Available Studies were under taken to assess the yield, earliness and insect pest resistance of some newly developed okra leaf strains compared with normal leaf commercial varieties CRIS-9 and NIAB-78. Accordingly, almost all the okra leaf mutants yielded better than NIAB-78. Out of nine okra leaf strains, three were better yields, two equally good and four gave low yield than CRIS-9 the second check variety. Highest yielding okra leaf strain produced 27 and 35 percent higher yield than CRIS-9 and NIAB-78 respectively. Okra leaf strains were documented as early maturing, whitefly tolerant and boll rot disease resistant. Realizing the better performance of okra leaf strains with reduced insecticide applications, the commercial utilization of these varieties in Sindh can not be ignored and may prove better replacement of current cultivars.

  10. Okra Leaf Cotton, its Commercial Utilization in Sindh

    A. R. Soomro; A. W. Soomro; Mallah, G. H.; Memon, A. M.; Soomro, A. H.; A.D. Kalhoro

    2000-01-01

    Studies were under taken to assess the yield, earliness and insect pest resistance of some newly developed okra leaf strains compared with normal leaf commercial varieties CRIS-9 and NIAB-78. Accordingly, almost all the okra leaf mutants yielded better than NIAB-78. Out of nine okra leaf strains, three were better yields, two equally good and four gave low yield than CRIS-9 the second check variety. Highest yielding okra leaf strain produced 27 and 35 percent higher yield than CRIS-9 and NIAB...

  11. Study on the degumming process of Abelmoschus manihot (L. Medic fiber

    Chun-Yan Li

    2013-06-01

    Full Text Available Abelmoschus manihot (L. Medic has drawn much attention recently due to its potential beneficial health effects. The roots, flowers and seeds of A.manihot play an important role as medical materials. In this paper, the result of the chemical composition testing shows that the main constituent of A.manihot bast of the stems is cellulose (41.80 %. A.manihot fibers are extracted from A.manihot bast by the treatment of degumming. In the degumming process, the key parameters have been optimized. The results of FTIR and XRD analysis indicated that hemicellulose, lignin, pectin and other impurities can be effectively removed by this degumming process, and the main components of A.manihot fiber was cellulose much the same as cotton, the crystallinity of A.manihot bast and fiber were 50.45% and 63.73% respectively.

  12. Morphological and biochemical responses of Abelmoschus esculantus (L.) Moench to zinc nanoparticles

    Gokak, I. B.; Taranath, T. C.

    2015-01-01

    The increasing application and use of nanoparticles are directly related to their release in the environment. There has arisen the necessity to study the interactions of nanoparticles with plants and other organisms. The present investigation is an attempt to evaluate the morphological and biochemical responses of Abelmoschus esculentus (L.) Moench to zinc nanoparticles treatment. Seeds were treated with zinc nanoparticles at concentrations of 50 100, 200, 500 ppm. The morphological and biochemical responses recorded include germination percentage, root, shoot length, number of root hairs and number of leaves. Chlorophyll, protein, proline and carbohydrate contents in the leaves of 30-days-old treated plants were estimated. A greater amount of total dissolved solids (TDS) leached out from the seeds soaked in different concentrations of Zn nanoparticles compared to the control. Zinc nanoparticles executed a positive impact on the seed germination. The seed germination percentage increased in all the treatments compared to control. Increase in the chlorophyll and protein content was also observed in the treated plants. The proline content increased in treated plants indicating the stress. The carbohydrate content of leaves decreased drastically in response to the treatment.

  13. Weed Species Distribution of Juvenile Oil Palm Tree (Elaeis guineensis Intercropped with Maize (Zea mays, Okra (Abelmoshus esculentus and Pepper (Capsicum anuum var. abbreviatum

    Ayodele Samuel OLUWATOBI

    2014-12-01

    Full Text Available This field experiment was carried out to evaluate the weed species distribution in the experimental plots of an intercrop of juvenile oil palm trees (Elaeis guineensis Jacq. with maize (Zea mays Linn., okra (Abelmoschus esculentus Moench and pepper (Capsicum annuum var. abbreviatum. This was carried out during the cropping season between July and October 2012. The crops were intercropped with the juvenile oil palm trees of about 3-years-old. The experiment was laid out in a completely randomized block design with five (5 replicates. The treatments comprised of intercropping distances of 1 m, 2 m and 3 m each for the three (3 crops (maize, okra and pepper and a plot for each of the three (3 arable crops without oil palm trees as control. Weed species distribution was carried out in each of the plots to determine the Simpson’s Diversity Index (D, Simpson’s Index of Diversity (1-D and Simpson’s Reciprocal Index (1/D. Weed species’ frequency, density, relative density, relative frequency, importance value, abundance, dominance and relative dominance were also computed from data collected at 3WAP and 6WAP. The results showed that the control plot has the highest weed species distribution at 3WAP having the lowest Simpson’s Diversity Index (D of 0.0930. Okra plot has the least weed species distribution with the highest Simpson’s Diversity Index (D of 0.2726. At 6WAP, the pepper plot has the highest weed species distribution having the lowest Simpson’s Diversity Index (D of 0.1741. Control plot has the least weed species distribution with highest Simpson’s Diversity Index (D of 0.2831.

  14. Genetic variability and heritability in cultivated okra [Abel moschus esculentus (L.) Moench

    Nwangburuka, C. C.; Denton, O. A.; Khinde, O. B.; Ojo, D. K.; Popoola, A. R.

    2012-11-01

    Twenty-nine okra accessions from different agro-ecological regions in Nigeria were grown during the rainy and dry seasons, between 2006 and 2007 at Abeokuta (derived savanah) and Ilishan (rainforest) and assessed to determine their genetic variability, heritability and genetic advance from eight yield related characters. The experiment was laid out in a Randomized Complete Block Design with five replications. There was high genotypic coefficient of variability, % broad-sense heritability and genetic advance in traits such as plant height (26.2, 90.7, 51.5), fresh pod length (23.9, 98.5, 48.8), fresh pod width (23.9, 98.5, 48.8), mature pod length (28.6, 98.5, 52.3), branching per plant (29.3, 82.3, 54.8) and pod weight per plant (33.9, 90.0, 63.3), suggesting the effect of additive genes and reliability of selection based on phenotype of these traits for crop improvement. The positive and significant phenotypic and genotypic correlation between plant height at maturity, fresh pod width, seeds per pod and pods per plant, branches per plant with seed weight per plant and pod weight per plant, suggests that selection on the basis of the phenotype of these characters will lead to high seed and pod yield in okra. (Author) 26 refs.

  15. Systematic Implications of Seed Coat Morphology in Malvaceae

    Salah M. I. El Naggar

    2001-01-01

    Seed morphological characters and seed coat sculpture of 14 species of Malvaceae were examined to assess systematic implications of seed coat sculpture. These taxa are Malva aegyptia L., M. parviflora L., M. neglecta Wallr., M. nicaeensis All., M. sylvestris L., Lavatera cretica L., Alcea rosea All., Sida alba L., Abutilon theophrasti Medicus, A. pannosum (G. Forster) Schlecht., Abelmoschus esculentus (L.) Moench. Hibiscus sabdariffa L., H. trionum L., and Gossypium barbadense (L.). Seed shap...

  16. INFLUENCE OF COVER CROPS IN ROTATION ON IMPROVING OKRA (ABELMOSCHUS ESCULENTUS L.) YIELD AND SUPPRESSING PARASITIC NEMATODES

    The influence of growing and incorporating summer cover crops on subsequent vegetable crop production and on population densities of the root-knot nematode (Meloidogyne incognita) was investigated in field and pot experiments at Homestead, Florida. The cover crops utilized in the field and pot exper...

  17. Osmotic adjustment and the growth response of seven vegetable crops following water-deficit stress. [Phaseolus vulgaris L. ; Beta vulgaris L. ; Abelmoschus esculentus; Pisum sativum L. ; Capsicum annuum L. ; Spinacia oleracea L. ; Lycopersicon esculentum Mill

    Wullschleger, S.D. (Oak Ridge National Lab., TN (United States)); Oosterhuis, D.M. (Univ. of Arkansas, Fayetteville (United States))

    1991-09-01

    Growth-chamber studies were conducted to examine the ability of seven vegetable crops- Blue Lake beam (Phaseolus vulgaris L.) Detroit Dark Red beet (Beta vulgaris L.) Burgundy okra (Abelmoschus esculentus) (Moench), Little Marvel pea (Pisum sativum L), California Wonder bell pepper (Capsicum annuum L), New Zealand spinach (Spinacia oleracea L), and Beefsteak tomato (Lycopersicon esculentum Mill.) - to adjust osmotically in response to water-deficit stress. Water stress was imposed by withholding water for 3 days, and the adjustment of leaf and root osmotic potentials upon relief of the stress and rehydration were monitored with thermocouple psychrometers. Despite similar reductions in leaf water potential and stomatal conductance among the species studied reductions in lead water potential an stomatal conductance among the species, crop-specific differences were observed in leak and root osmotic adjustment. Leaf osmotic adjustment was observed for bean, pepper, and tomato following water-deficit stress. Root osmotic adjustment was significant in bean, okra, pea and tomato. Furthermore, differences in leaf and root osmotic adjustment were also observed among five tomato cultivars. Leaf osmotic adjustment was not associated with the maintenance of leaf growth following water-deficit stress, since leaf expansion of water-stressed bean and pepper, two species capable of osmotic adjustment, was similar to that of spinach, which exhibited no leaf osmotic adjustment.

  18. Pectin from Abelmoschus esculentus: optimization of extraction and rheological properties.

    Chen, Yi; Zhang, Jian-Guo; Sun, Han-Ju; Wei, Zhao-Jun

    2014-09-01

    Response surface methodology (RSM) was applied to optimize the parameters of pectin extraction from okra pods. The extracted okra pectin was then investigated by steady-shear and oscillatory rheological measurements. Statistical analysis showed that the linear term of the liquid-solid ratio, the quadratic term of the pH, and the linear term of the extraction time showed highly significant effects on pectin yield. The optimal extraction conditions that maximized the pectin yield within the experimental range of the variables researched were a pH of 3.9, an extraction time of 64 min, an extraction temperature of 60°C, and a liquid-solid ratio of 42:1. Under these conditions, the pectin yield was predicted to be 2.71%. At a liquid-solid ratio less than 2.5% w/w in aqueous solution, the pectin extracted from okra presented non-Newtonian shear-thinning behavior and could be well described by the Cross model. The okra pectin showed predominantly viscous responses (G'

  19. Preventative and Curative Effects of Several Plant Derived Agents Against Powdery Mildew Disease of Okra

    Moustafa Hemdan Ahmed MOHARAM

    2012-08-01

    Full Text Available The preventative and curative effects of some plant derived agents based on plant extracts or essential oils were studied at different concentrations against Erysiphe cichoracearum DC. ex Merat, the causal pathogen of okra powdery mildew by the detached leaf-disk and potted plants bioassays. Through detached leaf-disk assay, the highest mean preventative effect (97.74% was recorded by neem seed oil followed by jojoba oil (89.82% and extract of Rynoutria sachalinensis (82.77%. Neem seed oil at 1% was the most effective agent followed by jojoba oil and extract of R. sachalinensis at 1.5% and 2%, respectively, where they suppressed E. cichoracearum completely. Potted plants assay revealed that neem seed oil, jojoba oil and extract of R. sachalinensis as well as the fungicide (active ingredient dinocap showed higher preventative efficacy at all leaf olds treated after 7 and 14 days of inoculation as compared with extracts of henna and garlic. Moreover, the preventative efficacy partly remained apparent after 14 days of inoculation at all leaf olds tested. In field trials through 2010 and 2011 growing seasons, when the first symptoms of powdery mildew appeared naturally, 1.5% jojoba oil, 2% extract of R. sachalinensis and 1% neem seed oil were sprayed individually twice on grown plants to evaluate their efficacy on controlling powdery mildew, growth and yield of okra. Resulted showed that neem seed oil was the most effective agent and highly decreased the disease severity to 29.92%, recorded the highly curative effect (68.15% and also improved plant growth and pods yield.

  20. Whitefly population dynamics in okra plantations Dinâmica populacional de mosca-branca em quiabo

    Germano Leão Demolin Leite

    2005-01-01

    Full Text Available The control of whitefly Bemisia tabaci (Gennadius biotype B (Hemiptera: Aleyrodidae on okra (Abelmoschus esculentus L. consists primarily in the use of insecticides, due to the lack of information on other mortality factors. The objective of this study was to evaluate the spatial and temporal population dynamics of the whitefly B. tabaci biotype B on two successive A. esculentus var. "Santa Cruz" plantations. Leaf chemical composition, leaf nitrogen and potassium contents, trichome density, canopy height, plant age, predators, parasitoids, total rainfall and median temperature were evaluated and their relationships with whitefly on okra were determined. Monthly number estimates of whitefly adults, nymphs (visual inspection and eggs (magnifying lens occurred on bottom, middle and apical parts of 30 plants/plantation (one leaf/plant. Plants senescence and natural enemies, mainly Encarsia sp., Chrysoperla spp. and Coccinellidae, were some of the factors that most contributed to whitefly reduction. The second okra plantation, 50 m apart from the first, was strongly attacked by whitefly, probably because of the insect migration from the first to the second plantation. No significant effects of the plant canopy on whitefly eggs and adults distribution were found. A higher number of whitefly nymphs was found on the medium part than on the bottom part.O controle da mosca-branca Bemisia tabaci (Gennadius biótipo B (Hemiptera: Aleyrodidae em quiabeiro (Abelmoschus esculentus L. consiste principalmente no uso de inseticidas, em virtude da falta de informação sobre outros fatores de mortalidade. O objetivo deste trabalho foi compreender a dinâmica populacional, espacial e temporal da mosca-branca em dois cultivos sucessivos de quiabeiro "Santa Cruz". Avaliaram-se a composição química foliar, os níveis foliares de nitrogênio e de potássio, a densidade de tricomas, a altura de dossel, a idade de planta, predadores, parasitóides, pluviosidade total, temperatura média e suas relações com a mosca-branca em quiabeiro. Estimou-se, mensalmente, o número de adultos e de ninfas (inspeção visual e de ovos (lentes de aumento de mosca-branca ocorridos nas folhas (uma folha/planta localizadas nas partes basal, mediana e apical de 30 plantas/plantação. Os fatores que mais contribuíram com a redução da população mosca-branca foram a senescência de plantas e inimigos naturais, principalmente Encarsia sp., Chrysoperla spp. e Coccinellidae. O segundo cultivo de quiabo, a 50 m do primeiro, foi altamente atacado pela mosca-branca, provavelmente pela migração dos insetos do primeiro para o segundo cultivo. Não foi detectado efeito significativo do dossel de plantas sobre ovos e adultos. Foi encontrado maior número de ninfas na parte mediana do que na parte basal das plantas.

  1. Effect of x-irradiation on physiological and morphological variability in Abelmoschus esculentus (L.) Moench

    The variations induced by x-irradiation of seeds of Abelmoschus esculentus in x1, generation have been studied. Percentage of germination showed a slight increase in treatments at 1 to 2kR, while at the higher doses there was a gradual decrease in germination. Seedling growth measured at regular intervals of 6 hr up to 96 hr, showed that growth rate was reduced and germination delayed at doses of 8 to 10 kR. Lateral root number and length were not significantly effected by the dosage rate. Total chlorophyll content of leaves was reduced by 50% at 8 kR treatment, while an increased chlorophyll metabolism was observed at doses of 4 and 7 kR. Increasing dose rate caused a gradual decrease in plant height. Early flowering was induced in 8,9 and 10 kR treatments, while flowering was delayed in the 5 and 6 kR treat samples. Average pod length did not show significant variations between the different dose rates as was also the stomatal index. Increased seed weight was observed in samples treated above 8 kR. (author)

  2. 7 CFR 319.37-6 - Specific treatment and other requirements.

    2010-01-01

    ... noxious weeds listed in 7 CFR 360.200. Hibiscus spp. (hibiscus, rose mallow) seeds All, with the exception... necessary: Seed/bulb Country/locality Pest(s) for which treatment is required Abelmoschus spp. (okra) seeds..., Hungary, Iran, Israel, Italy, Kazakhstan, Kyrgyzstan, Republic of Moldova, Morocco, Portugal, Serbia...

  3. Studies on the control of insect pests in vegetables (Okra, Tomato, and Onion) in Sudan with special reference to Neem-preparations

    Ahmed, Mohamed Mudathir Mohamed

    2000-01-01

    Field trials were conducted for three years (1997-1999) to study the effectiveness of different neem formulations against the major insect pests complex and on yield of okra (Hibiscus esculentus), tomato (Lycopersicon escculentum), and onion (Allium cepa). Neem kernel powder was applied into the soil (NKPS), sprayed as water extract on the target plants (ANKE) or applied as water extract into the soil (ANKES). The registered German formulated neem seed extract ( NeemAzal), includes...

  4. Okra yield fertilized with bovine manure and biofertilizer

    Ademar Pereira de Oliveira

    2013-12-01

    Full Text Available The use of bovine manure becomes an useful and economic practice for the small and medium producers of vegetables, and the okra plant normally demands high doses of organic fertilizers. This study was carried out, from January to July 2011, at the Federal University of Paraíba, in Areia city - PB, aiming to evaluate the effect of bovine manure and biofertilizer on the productive behavior of the okra plant. The experimental design used was randomized blocks, with four repetitions in factorial scheme 6 x 2, with the doses factors of bovine manure (0, 10, 20, 30, 40 and 50 t ha-1 with and without biofertilizer. The average mass of commercial fruit of okra, with and without biofertilizer was 18 and 16.5 g, respectively, in the doses of 27.5 and 60 t ha-1 of manure. The number of fruit plant-1 without biofertilizer was 30 fruits plant-1 of okra in the dose of 60 t ha-1 and with biofertilizer, the number of fruits plant-1 was 33 fruits in the dose of 28 t ha-1 of bovine manure. The productivity of commercial fruits of okra without biofertilizer was 20.4 t ha-1 and 22 t ha-1 with biofertilizer, respectively, in the doses of 60 and 31 t ha-1 of bovine manure.

  5. Impact of poultry manure on fruit yield and yield components of inland valley okra (Albelmoschus esculentus L. in a forest – Savannah transition ecology of Nigeria

    Olaiya A. O.

    2015-10-01

    Full Text Available A field experiment was conducted in an inland valley at the Federal University of Agriculture, Abeokuta, Nigeria in 2009 and 2010 to study the response of okra (Abelmoschus esculentus to different rates of poultry manure. The poultry manure rates were 12.5, 25, 37.5 and 120 kgha-1 NPK (20-10-10 as check and no amendment as control. All these were arranged in Randomized Complete Block Design with five treatments and three replicates. Data collected on growth and yield parameters were analysed using general linear model and treatment means were separated using Duncan’s Multiple Range Test. Application of poultry manure at 37.5 kgha-1 produced the tallest plant (58.1 cm which was significantly different from the rest treatments, it was followed by 25 kgha-1 (55.3 cm and 12.5 kgha-1 (52.5 cm and shortest plants were observed in the control. NPK produced the largest leaf area (1.36 m2plant-1 compared to other treatments. Fruit length and number of fruit plant-1 were similar among plants treated with 25 and 37.5 kgha-1. Similarly, fresh fruit weight was similar among the plants treated with 25 and 37.5 kgha-1 in the two years. It is therefore concluded that 25 kgha-1 of poultry manure may be adequate to meet the okra nutritional need in the partially depleted inland valley and can be produced twice using the residual moisture in dry season.

  6. Avaliação da resistência de genótipos de quiabeiro à infestação por Meloidogyne incognita raça 2 e M. javanica Resistance of okra genotypes to Meloidogyne incognita race 2 and M. javanica

    Gilmar Efrem Martinello

    2001-07-01

    Full Text Available Vinte e dois genótipos de quiabeiro (Abelmoschus spp. foram avaliados para resistência à Meloidogyne incognita raça 2 e M. javanica. Estes materiais, mantidos no Banco de Germoplasma da Universidade Estadual do Norte Fluminense, constam de quatro espécies selvagens Abelmoschus manihot (CGO 8655, A. caillei (CGO 8656, A. tetraphyllus (CGO 8657 e A. ficulneus (CGO 8658; 16 linhas de A. esculentus na sétima geração de autofecundação, resultantes de inter-cruzamentos do genótipo PI-357991 (supostamente resistentes a nematóides com as cultivares Piranema e Santa Cruz 47. Essas cultivares serviram como padrão de suscetibilidade. As plantas foram inoculadas separadamente com 5.000 ovos/segundo estádio juvenil (J2 de M. incognita raça 2 e M. javanica. Não houve diferença significativa com relação à resistência dos materiais a M. javanica. Os genótipos descendentes de 'PI-357991' mostraram-se segregantes para a reação de resistência, sendo que entre estes 'CGO 8180A7' apresentou o maior nível de tolerância à raça 2 de M. incognita. As espécies silvestres também não mostraram alguma fonte de resistência. As altas temperaturas ocorridas no período do experimento, podem ter aumentado a suscetibilidade dos genótipos aos dois patógenos.Twenty two okra genotypes were evaluated for resistance to M. incognita race 2 and M. javanica. The Universidade Estadual do Norte Fluminense (Brazil maintains okra genotypes in the germplasm collection, consisting of four wild Abelmoschus species and 16 F7 lines obtained from crosses between PI-357991 (considered resistant to root-knot nematodes and the local cvs, Piranema and Santa Cruz 47 (both susceptible to nematodes. No resistance was observed among okra genotypes to infection by M. javanica. The 16 F7 lines segregated for pathogenic reaction, and the CGO 8180A7 presented the highest resistance level to M. incognita race 2. The wild species did not show genetic resistance to both pathogens. High temperature occurring during experimental period could have increased the genotype susceptibility to the pathogens.

  7. CHARACTERIZATION AND EVALUATION OF OKRA GUM AS A TABLET BINDER

    N Tavakoli

    2008-01-01

    Full Text Available The type and amount of binders decisively influence the characteristics of tablets prepared by wet granulation procedure. Commonly used binders like acacia, gelatin, starch and hydrolyzed starch have natural origin. The aim of this study was to evaluate the effectiveness of a new binder extracted from Hibiscus esculentus (Okra gum in tabletting. Okra gum was extracted from the pods of Okra fruit by maceration in distilled water followed by filtration of viscous solution as well as precipitation of gum extract by using acetone. To evaluate the binder effectiveness, two models, including a placebo formulation (lactose and a drug formulation (Acetaminophen, Ibuprofen, and/or Calcium acetate were evaluated. Granules were prepared by different concentrations (0.5-6 %w/w of Okra gum and tabletted using a Kilian single punch press. Cornstarch (12.5 % w/w and P.V.P (22 %w/w were employed as the standard binders for comparison. The physical properties of the granulates and those of the tablets including disintegration time and dissolution rate were studied. The properties of placebo granulates (bulk and tapped density, granule strength, flowability as well as those of tablets (hardness, friability, disintegration time were generally good. Moreover, the physical properties of Ibuprofen and Calcium acetate tablets containing Okra gum showed sufficient hardness, desirable disintegration time and low friability. The percent of drug released after 45 minutes were 15 %, 44 % and 96 % for Acetaminophen, Ibuprofen and Calcium acetate tablets, respectively..Okra gum produces some tablet formulations with good hardness and friability. However, this binder prolongs the dissolution rate of some slightly soluble drugs and hence may be good candidate for sustained release formulations.

  8. Induced mutation breeding for resistance to yellow vein mosaic virus in okra

    Seeds of Annie and Okura okra varieties were irradiated by gamma rays to induce mutations for resistance to yellow vein mosaic virus disease (YVMD). In experiment I, seeds were irradiated at 400, 600 and 800 Gy and then planted at Huaysai King's Project, Petchaburi Province. Plants with a good plant type and green pods were selected for M2 generation. M3 plants were grown at Phichit Horticultural Research Center (PHRC) where YVMD was seriously widespread. 33 plants without disease symptoms were selected for further screening. By using white fly transmission under greenhouse conditions, only four lines showed no disease symptoms. They were transplanted to the field at PHRC. Only Okura irradiated at 400 Gy, designated Rd53-3 showed disease resistance. Subsequent selections were conducted under greenhouse and field conditions up to M7 generation. Twelve resistant lines showing uniformity of plant type were selected for yield trial observation. All bore reasonable yield but their fruit shape was not suitable for the export market. They will be used as parental lines in further breeding programs. In experiment II, seeds of Annie and Okura were irradiated at doses of 400 and 600 Gy and then planted at Huaysai. M2 seeds were collected by two methods, the pedigree method by collecting seeds from selected healthy plants, and then planted them at Huaysai. The other method was bulk selection and then seeds were planted at Huaysai and PHRC. The M3 generation was screened for disease resistance under greenhouse and field conditions. The plants from bulk selection were all infected. By pedigree selection, 34 and 35 lines of Annie and Okura, respectively were obtained. Screening for okra YVMD resistance under greenhouse and field conditions in M4 generation, it was found that only one plant of Okura irradiated at 400 Gy designated B-21, showed no disease symptoms. Disease resistance screenings under greenhouse and field conditions were performed during M5 to M7 generation. Ten lines selected from B-21 showed a good plant type coupled with a desirable fruit shape. They will be used for further yield trials. (author)

  9. 77 FR 6772 - United States Standards for Grades of Okra

    2012-02-09

    ... Federal Marketing Orders or U.S. Import Requirements no longer appear in the Code of Federal Regulations... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Agricultural Marketing Service United States Standards for Grades of Okra AGENCY: Agricultural...

  10. Epidemiology of Okra Yellow Vein Mosaic Virus (OYVMV) and Its Management through Tracer, Mycotal and Imidacloprid

    Muhammad Irfan Ali; Muhammad. Aslam Khan; Abdul Rashid; Muhammad Ehetisham-ul-haq; Muhammad Talha Javed; Muhammad Sajid

    2012-01-01

    Field trials were conducted to evaluate the epidemiological factors of okra yellow vein mosaic virus (OYVMV). Four varieties of okra were subjected to screening and evaluation of the chemicals. The variety Saloni F1 was highly resistant while Subz Pari was moderately resistant. Diksha was the tolerant variety while Lush Green was moderately susceptible. The chemical Imidacloprid was most effective to control whitefly population and okra yellow vein mosaic virus (OYVMV). Correlation of environ...

  11. Determination of molecular weights and monosaccharide compositions in Abelmoschus manihot polysaccharides

    Wang, Xuemei; Wang, Yingying; Wu, Mimi; Zhang, Xiuzhen

    2012-09-01

    Abelmoschus manihot polysaccharide, AMP-1, AMP-2, AMP-3, and AMP-4, were purified from four kinds of Abelmoschus manihot gum (AMG). The molecular weights and monosaccharide compositions of AMP-1, AMP-2, AMP-3 and AMP-4 were characterized by gel permeation chromatography, Fourier transform-infrared spectroscopy and high performance anion-exchange chromatography with pulsed amperometric detection. Results indicated that the molecular weights of AMP-1, AMP-2, AMP-3, and AMP-4 were approximately 3.91 × 103, 5.36 × 105, 3.87 × 103, and 5.12 × 105 Da, respectively. The Abelmoschus manihot polysaccharide was mainly composed of galactose, glucose and mannose with the molar ratios at 0.29: 1.00: 0.41 (AMP-1), 0.56: 0.13: 1.00 (AMP-2), 0.10: 1.00: 0.11 (AMP-3) and 0.55: 0.17: 1.00 (AMP-4), respectively.

  12. Polysaccharide extraction from Abelmoschus esculentus: optimization by response surface methodology.

    Samavati, Vahid

    2013-06-01

    Crude polysaccharide extraction from the Iranian Abelmoschus esculentus was performed using water decoction. Response surface methodology (RSM), based on a five level, four variable central composite rotatable design (CCRD), was employed to obtain the best possible combination of extraction time (X1: 0.5-6.5 h), extraction temperature (X2: 80-100 °C), number of extraction (X3: 1-5), and water to the raw material ratio (X4: 4-28) for maximum polysaccharide extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: extraction time of 4.94 h, extraction temperature of 94.97 °C, number of extraction of 4, and the ratio of water to raw material of 21.74. Under these conditions, the experimental yield was 16.895±0.29%, which is well in close agreement with the value predicted by the model 16.916%. PMID:23618310

  13. 76 FR 64001 - United States Standards for Grades of Frozen Okra

    2011-10-17

    ...The Agricultural Marketing Service (AMS) of the Department of Agriculture (USDA) has revised the United States Standards for Grades of Frozen Okra. The grade standards for frozen okra have been changed from a ``variables score point'' system to an ``individual attributes'' grading system. The ``dual grade nomenclature'' has been replaced with single letter grade designations and editorial......

  14. Proximate Composition of Selected Congo Oil Seeds and Physicochemical Properties of the Oil Extracts

    J.M. Nzikou

    2010-01-01

    Proximate composition, energy content and mineral concentrations of oil seeds which can be usedin the preparation of Congolese diets were investigated. The paper also reports the physicochemicalcharacteristics of the oil extracts from the seeds. Moisture content (on dry weight basis) was highest(9.45 ± 0.8%) in gumbo (Abelmoschus esclentus) seeds (AES), followed by amarenthus hybridus seeds (AHS)(9.07 ± 0.84%) but was lowest (4.13 ± 0.24%) in terminalia catappa seeds (TCS). Ash was highest(7....

  15. Analysis and comparison of the active components and antioxidant activities of extracts from Abelmoschus esculentus L

    Haibing Liao

    2012-01-01

    Full Text Available Background: Abelmoschus esculentus L. is a healthy vegetable belonging to the family Malvaceae. This article reports the contents of total phenolics (TP and total flavonoids (TF in 80% methanol extracts of the flower (FL, fruit (FR, leaf (L, and seed (S of A. esculentus, and in 0, 10, 30, 50, and 70% methanol eluates (ME, through the HP-20 column chromatography of 80% of the methanol fruit extract after it is defatted with petroleum and extracted with ethyl acetate. All the names of the samples are shortened for AEE-FL, AEE-FR, AEE-L, AEE-S and 0% MEF-WE, 10% MEF-WE, 30% MEF-WE, 50% MEF-WE, 70% MEF-WE respectively. In addition, the effects of the aforementioned extracts on 1,1-Diphenyl-2-picryl-hydrazyl (DPPH radical-scavenging and on ferric reducing antioxidant power (FRAP have been evaluated. Materials and Methods: The antioxidant activity of the extracts and the enrichment fraction of A. esculentus were also evaluated by two assays, the DPPH radical-scavenging and ferric reducing antioxidant power (FRAP. The content measurement of TF and TP adopts the UV-2102 PCS method, and the measurement of the antioxidant activity adopts the Infinite M 200 method. Results: The experiment results show that all the different parts and different enrichment fractions of the water extracts of A. esculentus contain phenolics and flavonoids. Through the research of antioxidant activity we know that all the parts of the methanol extracts and different enrichment fractions of water extracts in the A. esculentus have the effect of scavenging free radicals, among which the antioxidant activity in the 50% MEF-WE part is the strongest. Here, the main components of antioxidant activity must be the flavonoids and phenolics, and furthermore, we know that there is a direct relationship between the contents of flavonoids and phenolics and the antioxidant activity. Conclusion: The study suggests that A. esculentus may be the potential rich source of natural antioxidant. The experiment result provided a scientific basis for the further research and development of A. esculentus.

  16. Plant Growth-Promoting Rhizobacteria Enhance Salinity Stress Tolerance in Okra through ROS-Scavenging Enzymes

    Habib, Sheikh Hasna; Kausar, Hossain; Saud, Halimi Mohd

    2016-01-01

    Salinity is a major environmental stress that limits crop production worldwide. In this study, we characterized plant growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and examined their effect on salinity stress tolerance in okra through the induction of ROS-scavenging enzyme activity. PGPR inoculated okra plants exhibited higher germination percentage, growth parameters, and chlorophyll content than control plants. Increased antioxidant enzyme activities (SOD, APX, and CAT) and upregulation of ROS pathway genes (CAT, APX, GR, and DHAR) were observed in PGPR inoculated okra plants under salinity stress. With some exceptions, inoculation with Enterobacter sp. UPMR18 had a significant influence on all tested parameters under salt stress, as compared to other treatments. Thus, the ACC deaminase-containing PGPR isolate Enterobacter sp. UPMR18 could be an effective bioresource for enhancing salt tolerance and growth of okra plants under salinity stress. PMID:26951880

  17. Plant Growth-Promoting Rhizobacteria Enhance Salinity Stress Tolerance in Okra through ROS-Scavenging Enzymes.

    Habib, Sheikh Hasna; Kausar, Hossain; Saud, Halimi Mohd

    2016-01-01

    Salinity is a major environmental stress that limits crop production worldwide. In this study, we characterized plant growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and examined their effect on salinity stress tolerance in okra through the induction of ROS-scavenging enzyme activity. PGPR inoculated okra plants exhibited higher germination percentage, growth parameters, and chlorophyll content than control plants. Increased antioxidant enzyme activities (SOD, APX, and CAT) and upregulation of ROS pathway genes (CAT, APX, GR, and DHAR) were observed in PGPR inoculated okra plants under salinity stress. With some exceptions, inoculation with Enterobacter sp. UPMR18 had a significant influence on all tested parameters under salt stress, as compared to other treatments. Thus, the ACC deaminase-containing PGPR isolate Enterobacter sp. UPMR18 could be an effective bioresource for enhancing salt tolerance and growth of okra plants under salinity stress. PMID:26951880

  18. Utilization of Degraded Chitosan for Growth Promoter and Blossom Blight Disease Controls in Okra

    The experiment focus on the comparison of the effect of gamma radiation and chemical reagent on degradation of chitosan and preliminary test of degraded chitosan with different molecular weight as plant growth promoter of okra in potting experiment. (author)

  19. Plant Growth-Promoting Rhizobacteria Enhance Salinity Stress Tolerance in Okra through ROS-Scavenging Enzymes

    Sheikh Hasna Habib; Hossain Kausar; Halimi Mohd Saud

    2016-01-01

    Salinity is a major environmental stress that limits crop production worldwide. In this study, we characterized plant growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and examined their effect on salinity stress tolerance in okra through the induction of ROS-scavenging enzyme activity. PGPR inoculated okra plants exhibited higher germination percentage, growth parameters, and chlorophyll content than control plants. Increased antioxidant enzym...

  20. First Record of Cercospora Leaf Spot Disease on Okra Plants and its Control in Egypt

    Eman S.H. Farrag

    2011-01-01

    During June to September 2008, okra plants (Hibiscus esculentus L.) in Kafr El-Sheikh Governorate exhibited typical symptoms of Cercospora Leaf Spot (CLS) at different locations. Symptoms of infected okra leaves firstly started as light brown spots then turned to purple and varying in size. The spots spread to cover large areas of infected leaves. In case of severe infection, spots joined together and formed patches. Later, leaves were dry and remained intact with stem of plant. Samples of di...

  1. Laboratory evaluation of interception and translocation of 131I in fenugreek and Okra plants

    The work reported here deals with the study of interception and translocation of airborne 131I in fenugreek (Trigonella foenum-graecum) and okra (Hibiscus esculentus), two very common vegetables in India. Activity was injected into the experimental chamber in the form of iodide aerosols having a size distribution of 0.3 to 5.0 μm (AMAD). Samples of plants were collected over a period of a few days at different time intervals after injection of the aerosols. Evaluation of interception with deposition and translocation of 131I was done from the activity measured in air and in plant parts. For the deposition factor, the values are 1.22 m3 kg-1 for fenugreek leaves, and 1.49 m3 kg-1 for the plant as a whole. For okra plants these values are 0.02 and 0.16 m3 kg-1 in edible okra and leaves, respectively. For the okra plant as a whole, the value is 0.19 m3 kg-1. The translocation factors vary from 0.62 to 0.86 and 0.47 to 0.87 for leaves and stem, respectively, in the case of fenugreek plants. For okra plants, the translocation factor varies from 0.21 to 0.82, 0.53 to 0.93, and 0.42 to 0.81 in edible okra, leaves, and stems, respectively. 13 refs., 6 figs., 2 tabs

  2. Comparative Effect of Insecticide and Blue Polythene Hanging on the Incidence and Severity of Okra Mosaic in the Growth of Okra Plant

    Md. Fazlul Haque; S.M. Asraful Islam; M. Moktar Alam; A.F.M.G. Faruque Hossain; M.K. Anam

    2002-01-01

    A field study was conducted to compare the efficacy of blue polythene hanging over the plant and spraying insecticide in reducing incidence and severity of okra mosaic under natural inoculated condition. Size of blue polythene strip was 0.04x3.5 m2 and six polythene strips were used per plot and the recommended dose of insecticide was 454 ml per acre. Blue polythene strips and insecticide reduced the infection of okra mosaic virus. The number of disease infected plants per plot and mos...

  3. Variability studies for yield and its contributing traits in okra

    Salesh Kumar Jindal, Deepak Arora and T R Ghai

    2010-12-01

    Full Text Available Twelve okra genotypes were crossed in diallel fashion excluding reciprocals to generate 66 one-way hybrids. All the F1’s along with their parentswere grown at Vegetable Research Farm of the Department of Vegetable Crops, Punjab Agricultural University, Ludhiana, Punjab during therainy season of 2004-05. High genotypic and phenotypic coefficients of variation were noticed for number of primary branches per plantindicating maximum variability among the different genotypes. High estimates of heritability coupled with high genetic advance obtained fornumber of branches per plant, total yield per plant and marketable yield per plant indicating presence of additive gene effects which indicated theeffectiveness of selection for these traits. Presence of high heritability coupled with low genetic advance for days to fruit picking, average fruitweight, plant height, internodal length, number of fruits per plant, fruit diameter and average fruit length revealed that straight selection haslimited scope for further improving these traits.

  4. Comparative Studies on the Effect of Sowing Dates and Spacing on the Growth and Yield of Okra in Different Years

    A.B.M. Sharif Hossain A. Wahab

    2003-01-01

    Full Text Available Field studies were carried out to evaluate the effect of sowing time and spacing on the growth and fruit yield of okra in 1999 and 2000 in the farmer = s field, Lakshmipur, Bangladesh. The highest plant height was recorded 87.5 cm when okra was sown on 5 March, 2000 at the spacing of 45 x 45 cm and the lowest height was 73.3 cm when okra was sown on 20 May, 1999 at the spacing of 35 x 40 cm. The maximum number of leaves per plant was observed when it was sown on 5 March 2000 and the minimum was when sown on 20 May, 1999. Moreover, the fruit yield, number of fruit picking per plant, fruit length and weight per fruit were higher when okra was sown on 5 March, 5 April and 5 May, 2000 spaced at 45 x 45 cm than when okra was sown on 20 March, 20 April and 20 May, 1999 spaced at 35 x 40 cm. The highest number of percent mosaic infected plant was counted when okra was sown on 20 May, 1999 at the spacing of 35 x 40 cm and the lowest was when okra was sown on 5 March, 2000 at the spacing of 45 x 45 cm. In addition to that the highest number of vector of okra mosaic disease was counted when okra was sown on 20 May, 1999 at the spacing of 35 x 40 cm and the lowest was when okra was sown on 5 March, 2000 at the spacing of 45 x 45 cm. Percent mosaic infected plants was highest in 1999 and the lowest was in 2000 when it was sown on 20 May and 5 March due to the presence of the number of vectors of okra mosaic disease.

  5. Molecular characterization of begomoviruses and DNA satellites associated with okra leaf curl disease in Cameroon.

    Leke, Walter N; Sattar, Muhammad N; Ngane, Emilia B; Ngeve, Jacob M; Kvarnheden, Anders; Brown, Judith K

    2013-06-01

    Okra leaf curl disease (OLCD) is the most important viral disease of okra in West Africa. In this study, a complex of begomoviruses and associated DNA satellites were identified in symptomatic okra plants from southwestern Cameroon. Sequence analyses showed that two of the plants (Lik1 and Njo5) were infected with a begomovirus being a recombinant of cotton leaf curl Gezira virus (CLCuGeV) and okra yellow crinkle virus (OYCrV). The recombinant genome shared highest nucleotide identity with isolates of CLCuGeV at 87.8% and is therefore considered to be member of a new begomovirus species, Okra leaf curl Cameroon virus (OLCuCMV). One plant (Mue5) was infected by a begomovirus with 95.8% nucleotide identy to CLCuGeV, while in the plants Lik1, Mue1 and Njo5, a begomovirus was identified showing highest nucleotide identity at 93.7% with OYCrV. The nucleotide comparisons and phylogenetic analyses suggest that these isolates represent new Cameroonian strains of CLCuGeV and OYCrV (CLCuGeV-CM and OYCrV-CM). Mixed infection of OLCuCMV and OYCrV-CM was found in two of the plants. A betasatellite and two divergent alphasatellites were also associated with the begomoviruses. The betasatellite was identified as cotton leaf curl Gezira betasatellite (CLCuGeB) with the highest nucleotide identity at 93.3% to other African isolates of CLCuGeB. The alphasatellites, herein named Alpha-1 and Alpha-2, shared 97.3% and 95.2% identity, respectively, with cotton leaf curl Gezira alphasatellite (CLCuGeA) and okra leaf curl Burkina Faso alphasatellite (OLCuBFA). These collective results emphasize the extent of diversity among okra-infecting begomovirus-satellite complexes in western Africa. PMID:23535770

  6. Influência da colonização micorrízica arbuscular sobre a nutrição do quiabeiro Influence of arbuscular mycorrhiza fungi on the nutrition of okra plant

    Ricardo Luís Louro Berbara

    1999-09-01

    Full Text Available Foram estudados em casa de vegetação alguns parâmetros de crescimento em plantas de quiabo (Abelmoschus esculentus (L. Moench cv. Piranema colonizadas por dois grupos de fungos micorrízicos arbusculares, com o objetivo de determinar a influência dos inóculos na nutrição e morfologia radicular do quiabeiro. Um grupo continha apenas esporos de Acaulospora longula (A enquanto o outro, esporos de oito espécies: Glomus occultum, Glomus aggregatum, Glomus microcarpum, Acaulospora longula, Acaulospora morrowae, Sclerocystis coremioides, Sclerocystis sinuosa, Scutellospora pellucida. As plantas foram submetidas a três níveis de P (0, 10 e 60 kg ha-1 de P2O5 e coletadas em três diferentes idades (22, 32 e 47 dias, com quatro repetições para cada tratamento. Foi determinado o acúmulo de N, P, K, e Mg na raiz e parte aérea, bem como o influxo médio desses elementos e a área radicular. Os resultados indicaram, além da resposta positiva do quiabeiro ao P, uma maior eficiência da inoculação com mistura de espécies apesar de o influxo médio, determinado aos 47 dias, apresentar maiores valores para o tratamento com A. longula.An experiment was carried out in greenhouse to determine the influence of inoculation of two groups of arbuscular mycorrhizae on the nutrition and radicular morphology of the okra plant (Abelmoschus esculentus (L. Moench cv. Piranema. One group had only Acaulospora longula spores and the other a spore mixture of eight species: Glomus occultum, Glomus aggregatum, Glomus microcarpum, Acaulospora longula, Acaulospora morrowae, Sclerocystis coremioides, Sclerocystis sinuosa, Scutellospora pellucida. The experiment was held in greenhouse conditions with three levels of P (0, 10 and 60 kg ha-1 of P2O5, three samplings dates (22, 32 and 47 days and four replications. The accumulation of N, P, K, Ca and Mg in roots and shoots, root area and their influx ratio were determined. The results made evident that the mixture of species was more efficient although there were higher ratios of influx with Acaulospora longula between 0 and 47th. day.

  7. Efeito da Incorporação de Folhas de Nim ao Solo sobre o Complexo Fusarium x Meloidogyne em Quiabeiro Effect of amendment of soil with neem leaf on Fusarium x Meloidogyne on okra

    Gilson Soares da Silva

    2008-12-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da incorporação de folhas frescas de nim (Azadirachta indica ao solo, sobre o complexo Fusarium x Meloidogyne em quiabeiro (Abelmoschus esculentum em um experimento realizado em condições de casa de vegetação. Os tratamentos constaram da adição de 25g ou 50g de folhas trituradas/kg de solo previamente autoclavado e inoculado com M. incognita, Fusarium oxysporum f. sp. vasinfectum, juntos e isoladamente, contidos em vasos com capacidade de 2 L. Solo sem folhas de nim serviu como testemunha. O experimento foi realizado seguindo um delineamento inteiramente casualizado com seis repetições, sendo cada repetição representada por um vaso com 5 plantas. As folhas foram incorporadas 30 dias antes do plantio e a avaliação deu-se 90 dias após o plantio, adotando-se a percentagem de plantas mortas como parâmetro para avaliar o efeito dos tratamentos. A incorporação de 50g de folhas frescas de nim foi eficiente para o controle de Meloidogyne e Fusarium isoladamente, bem como na interação desses patógenos. A incorporação de 25g de folhas de nim mostrou-se eficiente apenas para o controle de Meloidogyne isoladamente.The objective of this study was to investigate the effect of soil ammendment with neem (Azadirachta indica leaf on the complex Fusarium x Meloidogyne in okra (Abelmoschus esculentum in greenhouse conditions. The treatments consisted of the addition of 25g or 50g of leaves per kg of soil and infested with M. incognita and Fusarium oxysporum f. sp. vasinfectum, together and separately, contained in pots with capacity of 2 L. Soil without supplement it served as the control. The experimental design was completely randomized with six replications and five plants per pot. The leaves were incorporate 30 days before planting and the evaluation 90 days later. The percentagem of dead plants was used to evaluate the efficiency of neem. The incorporation of 50g of fresh neem leaves was efficient in the control of both Meloidogyne and Fusarium separately as well as in their interaction. The incorporation of 25g of neem leaves was shown efficient just for the control of Meloidogyne separately.

  8. Development of post-harvest protocol of okra for export marketing.

    Dhall, R K; Sharma, S R; Mahajan, B V C

    2014-08-01

    The study was carried out on the harvesting and handling methods of okra with the objective to maintain the best quality of pods from harvesting to end consumer especially for export marketing. For that purpose okra cv. 'Punjab-8' pods were harvested with minimum handling (least injuries to the pubescence on the ridges of pod) and normal handling (no safety taken to prevent injuries on pods). Pods were precooled at 15?±?1ºC, 90-95% RH; jumble packed in the CFB boxes of 2.0 Kg capacity and than stored at 8?±?1ºC, 90-95% RH. The quality parameters of okra namely texture, chlorophyll content, physiological loss in weight, rotting percentage and general appearance were studied. The pods harvested with minimum handling and field packaging can retain their green colour, crisp texture (maximum force to puncture pod?=?500.2 g) with minimum rotting (3.0%) and physiological loss in weight (15.8%) and good appearance upto 13 days of cold storage whereas normal handled pods can be stored upto 5 days at 8?±?1ºC, 90-95% RH and thereafter lost their general appearance on the 7th day of storage and were discarded. Therefore, in order to maintain high quality of okra from harvesting to the final destination (consumer), the okra pods should be harvested with minimum handling followed by field packaging in CFB boxes. PMID:25114358

  9. Comparison between the DNA Fingerprints Obtained from the Yellow Vein Mosaic Disease Tolerant Okra Mutants and Their Parental Variety

    The yellow vein mosaic disease (YVMD) is a widespread disease that is found among export orchards of okra. In this report, we studied gamma radiation-induced YVMD tolerant okra mutants and other commercial okra varieties at DNA level. We found that DNA extraction method that utilized sodium dodecyl sulfate and potassium acetate to precipitate other biomolecules was a suitable method to use for DNA finger printing of okra. The MFLP finger printing technique was superior to the AFLP technique in finding polymorphisms among different okra varieties. Also polymorphisms between the YVMD-tolerant mutant lines and their parental variety could be detected, indicating that gamma radiation could induce some changes at DNA level in these plants

  10. DESENVOLVIMENTO E PRODUÇÃO DO QUIABEIRO EM FUNÇÃO DAS DATAS DE PLANTIO GROWTH AND YIELD OF OKRA AS INFLUENCED BY PLANTING DATES

    Peter Ernst Sonnenberg

    2007-09-01

    Full Text Available

    A influência de quatro épocas de plantio (15 de maio, 15 de junho, 15 de julho e 15 de agosto de 1998 no desenvolvimento e na produção do quiabeiro (Abelmoschus esculentus (L Moench (cv. Santa Cruz 47 foi investigada em Goiânia, GO. O experimento foi conduzido na Escola de Agronomia e Engenharia de Alimentos da Universidade Federal de Goiás, em Latossolo Vermelho Amarelo, cultivado há muitos anos. O delineamento experimental foi o de blocos casualizados com quatro repetições. Foram encontradas diferenças significativas (Tukey 5% entre épocas de plantio, para as seguintes características: número de frutos produzidos durante as trinta primeiras colheitas, altura das plantas no início da colheita, número de ramos laterais e número de dias da semeadura ao início do florescimento, ao início da colheita e ao início da colheita em 75% das covas. A temperatura média dos meses seguintes às datas de plantio aumentou de maio para agosto. Observou-se uma redução do período da semeadura até o início do florescimento, até o início da colheita e até o início da colheita em 75% das covas. O número de ramos laterais também foi reduzido no mesmo período. Entretanto verificou-se aumento na altura das plantas e no rendimento das primeiras trinta colheitas.

    PALAVRAS-CHAVE: Abelmoschus esculentus; data de plantio; épocas de plantio.

    The influence of four sowing dates (May 15th, June 15th, July 15th and August 15th, 1998 on the growth and yield of okra (Abelmoschus esculentus (L. Moench (cv. Santa Cruz 47 was studied in Goiânia-GO. The experiment was conducted at the 'Escola de Agronomia e Engenharia de Alimentos, Universidade Federal de Goiás', on an oxisol which had been cropped for several years. The experimental design was a randomized block with four replications. Significant differences (Tukey 5% were found among sowing dates for the following traits: number of pods yielded during the first thirty harvests (105 days, plant height at the beginning of harvest, number of lateral branches, number of days from the sowing to the beginning of flowering, number of days from the sowing to the harvest of the first pod and number of days from the sowing to the beginning of harvest on 75% of the hills. The average temperature of the months following the sowing dates increased from May to August. The period from the sowing to the beginning of flowering, the beginning of harvest and the beginning of harvest on 75% of the hills was shortened and the number of lateral branches decreased from May to August. At the same time, the plant height and the yield of the first thirty harvests increased.

    KEY-WORDS: Abelmoschus esculentus; planting date; development yield.

  11. RELATIVE ECONOMIC VALUE OF MAIZE - OKRA INTERCROPS IN RAINFOREST ZONE, NIGERIA

    R ALABI

    2007-04-01

    Full Text Available This study determined the relative economic value of maize- okra intercrops in Edo State, Nigeria. The results of analysis show Land Equivalent Ratio (LER of 0.82, Relative Value Total (RVT of 0.99 and Relative Value of Intercropping (RVI of 1.10. Although LER and RVT show that monocrops of maize and okra have advantage over intercrops, RVI shows that the profi t from intercrop is 10% higher than monocrops. This is because of the attendant reduction in variable costs of labour and fertilizer that are associated with intercrops. The economic implication of this study is that any strategy that reduces cost of production in maize/ okra intercrops will increase its profi tability and attractiveness to farmers.

  12. Laboratory evaluation of interception and translocation of {sup 131}I in fenugreek and Okra plants

    Singhal, R.K.; Narayanan, U.; Bhat, I.S. [Bhabha Atomic Research Centre, Trombay (India)

    1994-11-01

    The work reported here deals with the study of interception and translocation of airborne {sup 131}I in fenugreek (Trigonella foenum-graecum) and okra (Hibiscus esculentus), two very common vegetables in India. Activity was injected into the experimental chamber in the form of iodide aerosols having a size distribution of 0.3 to 5.0 {mu}m (AMAD). Samples of plants were collected over a period of a few days at different time intervals after injection of the aerosols. Evaluation of interception with deposition and translocation of {sup 131}I was done from the activity measured in air and in plant parts. For the deposition factor, the values are 1.22 m{sup 3} kg{sup -1} for fenugreek leaves, and 1.49 m{sup 3} kg{sup -1} for the plant as a whole. For okra plants these values are 0.02 and 0.16 m{sup 3} kg{sup -1} in edible okra and leaves, respectively. For the okra plant as a whole, the value is 0.19 m{sup 3} kg{sup -1}. The translocation factors vary from 0.62 to 0.86 and 0.47 to 0.87 for leaves and stem, respectively, in the case of fenugreek plants. For okra plants, the translocation factor varies from 0.21 to 0.82, 0.53 to 0.93, and 0.42 to 0.81 in edible okra, leaves, and stems, respectively. 13 refs., 6 figs., 2 tabs.

  13. Screening of Okra Germplasm for Resistance to Yellow Vein Mosaic Virus under Field Conditions

    M.H. Rashid; Yasmin, L.; Kibria, M.G.; A.K.M.S.R. Mollik; S.M. Monowar Hossain

    2002-01-01

    Twelve okra germplasms were screened for resistance to okra yellow vein mosaic virus (YVMV) under field conditions. Lines OK-292 and OK-285 showed resistant to YVMV in both season and OK 315, OK 316 and OK 317 were found tolerant. The highest yield per hectare found in the line OK-292 (18.00 t ha-1) followed by OK-285 (16 t ha-1) and line OK-310 produced the lowest yield (9.85 t ha-1) followed by pusa (9.95 t ha-1).

  14. Responses of growth of lady’s fingers ([i]Abelmoschus esculentus [/i]L. to different treatments methods of dairy wastewater

    Rana Ibrahim Al-Dulaimi

    2014-03-01

    Full Text Available Introduction and objective. Water is one of the most important precious resources found on the earth, and are most often affected by anthropogenic activities and by industry. Pollution caused by human beings and industries is a serious concern throughout the world. Population growth, massive urbanization, rapid rate of industrialization and modern techniques in agriculture have accelerated water pollution and led to the gradual deterioration of its quality. A large quantity of waste water disposed of at sea or on land has caused environmental problems which have led to environmental pollution, economic losses and chemical risks caused by the wastewater, and its impact on agriculture. However, waste water which contain nutrients and organic matter has possible advantages for agricultural purposes. Therefore, the presented study was undertaken to assess the impact of Dairy Effluent (treated and untreated waste water on seed germination, seedling growth, dry matter production and the biochemical parameters of lady’s fingers ([i]Abelmoschus esculentus[/i] L.. Materials and methods. A field experiment in a green house was conducted to use raw and treated dairy wastewater for watering lady’s fingers (Abelmoschus esculentus L.. The plants were watered using (WW raw dairy wastewater, (T1 chemicals treatment, (T2 physical treatment, (T3 dilution method treatment and tap water (TW in pot experiments. Ten plants of each treatment /3 replicate were randomly selected and labelled for the collection of data. The data was collected sequentially, starting with chlorophyll content pre-harvest, vegetative qualities (shoot, root and seedling length and dry matter quality (shoot and root dry matter pos-tharvest. Results. The effect was seen on the germination seed and growth of the plant. The results showed inhibitory effect from dairy effluent (WW on seed germination and plant growth. Treatment with chemicals showed statistically significant differences with other treatments. Chemical treatment (TC2 at 20 mg/L Al2(SO43 and pH 6.5 improved all growth characteristics, compared with WW, and TW reached 85%, 70.8 cm, 28.6 cm, 99.4 cm, 65.36%, 15.86% and 3.543 Mgg FW for seed germination, shoot length, root length, seedling length, shoot dry matter, root dry matter and chlorophyll, respectively. Also, 25% concentration and 6.5 pH from the dilution method treatment improved all the qualities, but at a lower level. A maximum favourable effect was also observed in the (T2 physical treatment, and ranged from average to moderate in terms of impact. Conclusions. Thus, dairy effluent, after chemical treatment and proper dilution, can be used as a potential source of water for seed germination and plant growth in agricultural practices.

  15. The use of Hibiscus esculentus (Okra) gum in sustaining the release of propranolol hydrochloride in a solid oral dosage form.

    Zaharuddin, Nurul Dhania; Noordin, Mohamed Ibrahim; Kadivar, Ali

    2014-01-01

    The effectiveness of Okra gum in sustaining the release of propranolol hydrochloride in a tablet was studied. Okra gum was extracted from the pods of Hibiscus esculentus using acetone as a drying agent. Dried Okra gum was made into powder form and its physical and chemical characteristics such as solubility, pH, moisture content, viscosity, morphology study using SEM, infrared study using FTIR, crystallinity study using XRD, and thermal study using DSC and TGA were carried out. The powder was used in the preparation of tablet using granulation and compression methods. Propranolol hydrochloride was used as a model drug and the activity of Okra gum as a binder was compared by preparing tablets using a synthetic and a semisynthetic binder which are hydroxylmethylpropyl cellulose (HPMC) and sodium alginate, respectively. Evaluation of drug release kinetics that was attained from dissolution studies showed that Okra gum retarded the release up to 24 hours and exhibited the longest release as compared to HPMC and sodium alginate. The tensile and crushing strength of tablets was also evaluated by conducting hardness and friability tests. Okra gum was observed to produce tablets with the highest hardness value and lowest friability. Hence, Okra gum was testified as an effective adjuvant to produce favourable sustained release tablets with strong tensile and crushing strength. PMID:24678512

  16. Comparative Studies on the Effect of Sowing Dates and Spacing on the Growth and Yield of Okra in Different Years

    A.B.M. Sharif Hossain A. Wahab; Justus M. Onguso

    2003-01-01

    Field studies were carried out to evaluate the effect of sowing time and spacing on the growth and fruit yield of okra in 1999 and 2000 in the farmer = s field, Lakshmipur, Bangladesh. The highest plant height was recorded 87.5 cm when okra was sown on 5 March, 2000 at the spacing of 45 x 45 cm and the lowest height was 73.3 cm when okra was sown on 20 May, 1999 at the spacing of 35 x 40 cm. The maximum number of leaves per plant was observed when it was sown on 5 March 2000 and the min...

  17. First Record of Cercospora Leaf Spot Disease on Okra Plants and its Control in Egypt

    Eman S.H. Farrag

    2011-01-01

    Full Text Available During June to September 2008, okra plants (Hibiscus esculentus L. in Kafr El-Sheikh Governorate exhibited typical symptoms of Cercospora Leaf Spot (CLS at different locations. Symptoms of infected okra leaves firstly started as light brown spots then turned to purple and varying in size. The spots spread to cover large areas of infected leaves. In case of severe infection, spots joined together and formed patches. Later, leaves were dry and remained intact with stem of plant. Samples of diseased leaves were collected to isolate the causal organisms. Isolated fungi were purified using single spore culture technique. Developed fungus was identified as Cercospora sp. Fresen based on cultural and morphological characteristics after light microscope examination. Also, Alternaria alternate and Aspergillus niger were isolated as associated fungi. Pathoginicity test confirmed efficiency of Cercospora sp. to induce typical symptoms on okra plants compared with other fungi. Foliar application using different concentrations of Topsin M-70WP and lemongrass oil was significantly reduced disease incidence compared with control. According to the available literature, this is the first record of CLS on okra in Egypt under natural infection in the field.

  18. Persistence and effect of processing on reduction of chlorantraniliprole residues on brinjal and okra fruits.

    Vijayasree, V; Bai, Hebsy; Beevi, S Naseema; Mathew, Thomas Biju; George, Thomas; Xavier, George

    2015-05-01

    Dissipation and decontamination of chlorantraniliprole (Coragen 18.5 SC) in brinjal and okra fruits were studied following field application at single and double doses of 30 and 60 g ai ha(-1), and the residues of the insecticide was estimated using LC-MS/MS. Initial residues of chlorantraniliprole at single and double doses on the fruits of brinjal were 0.72 and 1.48 mg kg(-1), while on okra fruits, the residues were 0.48 and 0.91 mg kg(-1), respectively. The residues reached below detectable level of 0.01 mg kg(-1) on the 10th day. Half-life of chlorantraniliprole at 30 and 60 g ai ha(-1) on brinjal was 1.58 and 1.80 days with the calculated waiting period of 0.69 and 2.38 days, whereas on okra, the values were 1.60 and 1.70 and 0 and 1.20 days, respectively. The extent of removal of chlorantraniliprole using simple decontaminating techniques at 2 h and 3 days after spraying was 40.99-91.37% and 29.85-89.12%, respectively, from brinjal fruits and 47.78-86.10% and 41.77-86.48%, respectively, from okra fruits. PMID:25917186

  19. Factors affecting colonization and abundance of Aphis gossypii glover (hemiptera: aphididae on okra plantations Fatores que afetam a colonização e abundância de Aphis gossypii glover (Hemiptera: Aphididae em plantações de quiabeiro

    Germano Leão Demolin Leite

    2007-04-01

    Full Text Available The control of Aphis gossypii Glover (Hemiptera: Aphididae on okra Abelmoschus esculentus (L. (Malvaceae consist primarily in the use of insecticides, due to the lack of information on other mortality factors. The objective of this study was to determine the effects of predators and parasitoids, height of canopy, plant age, leaf areas, organic compounds leaves, levels of leaf nitrogen and potassium, density of leaf trichomes, total rainfall and median temperature on attack intensity of A. gossypii on two successive A. esculentus var. Santa Cruz plantations. Monthly number estimates of A. gossypii and natural enemies (visual inspection occurred on bottom, middle and apical parts of 30 plants/plantation (one leaf/plant. Plants senescence, leaf areas and natural enemies, mainly Adialytus spp., spiders and Coccinellidae, were some of the factors that most contributed to aphid reduction. A higher number of aphids was found on the bottom part than medium and apical parts of okra plants. Total rainfall can reduce the aphid population. Trichomes non-glandular or low density, organic compounds leaves and levels of N and K were not important for reducing aphid population.O controle de Aphis gossypii Glover (Hemiptera: Aphididae em quiabeiro Abelmoschus esculentus (L. (Malvaceae consiste principalmente no uso de inseticidas, em virtude da falta de informação sobre outros fatores de mortalidade. Objetivou-se com este estudo determinar os efeitos de predadores e parasitóides, altura de dossel, idade da planta, área foliar, compostos orgânicos foliares, níveis de nitrogênio e potássio, densidade de tricomas, pluviosidade e temperatura na intensidade de ataque de A. gossypii em dois cultivos sucessivos de Abelmoschus esculentus var. Santa Cruz. Estimou-se, mensalmente, o número de A. gossypii e de inimigos naturais (inspeção visual ocorridos nas folhas (uma folha/planta localizadas nas partes basal, mediana e apical de 30 plantas/plantação. Os fatores que mais contribuíram com a redução da população de pulgões foram a senescência de plantas, área foliar e inimigos naturais, principalmente Adialytus spp., aranhas e Coccinellidae. Maior número de pulgões foi observado na parte basal que na mediana e apical de plantas de quiabeiro. A pluviosidade total pode reduzir a população de pulgões. Tricomas tectores (não-glandulares ou baixa densidade destes, compostos orgânicos foliares e níveis de N e de K não foram importantes para a redução da população de pulgões.

  20. Natural mucoadhesive microspheres of Abelmoschus esculentus polysaccharide as a new carrier for nasal drug delivery.

    Sharma, Nitin; Kulkarni, Giriraj T; Sharma, Anjana; Bhatnagar, Aseem; Kumar, Neeraj

    2013-01-01

    This work describes the preparation and evaluation of mucoadhesive microspheres, using Abelmoschus esculentus polysaccharide as a novel carrier for safe and effective delivery of rizatriptan benzoate into nasal cavity. The polysaccharide was extracted from the fruit of A. esculentus and mucoadhesive microspheres were prepared by emulsification, followed by crosslinking using epichlorohydrin. Prepared microspheres were evaluated for size, morphology, swelling properties, mucoadhesive strength, encapsulation efficiency and drug release. Microspheres were found to release 50% of drug within 15 min and rest of the drug was released within 60 min. The drug release was found to decrease with increasing concentration of polysaccharide. To determine the retention time of the microspheres in the nasal cavity of rabbits, the microspheres were radiolabelled with (99m)Tc and subjected to gamma scintigraphy. The results showed a significant improvement in the nasal retention of the microspheres as compared to the aqueous solution of radiolabelled free-drug. PMID:23379506

  1. Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India

    Venkataravanappa, V.; Lakshminarayana Reddy, C. N.; Devaraju, A.; Jalali, Salil; Krishna Reddy, M.

    2013-01-01

    A begomovirus isolate (OY136A) collected from okra plants showing upward leaf curling, vein clearing, vein thickening and yellowing symptoms from Bangalore rural district, Karnataka, India was characterized. The sequence comparisons revealed that, this virus isolate share highest nucleotide identity with isolates of Cotton leaf curl Bangalore virus (CLCuBV) (AY705380) (92.8 %) and Okra enation leaf curl virus (81.1–86.2 %). This is well supported by phylogentic analysis showing, close cluster...

  2. Effect of blanching, dehydration method and temperature on the ascorbic acid, colour, sliminess and other constituents of okra fruit.

    Inyang, U E; Ike, C I

    1998-03-01

    Freshly harvested okra fruits were blanched in boiling water (with or without 0.2% sodium metablsulphite salt) and the effect of this treatment, dehydration methods and temperature on certain characteristics of okra investigated. Blanching resulted in the slight decrease in carbohydrate, fat, ash, colour components, ascorbic acid and viscosity. Besides this initial loss, blanching in sulphite solution led to the retention of more of the colour components and ascorbic acid during dehydration. All dehydrated okra samples had higher contents of carbohydrate, fat and ash but lesser contents of protein and ascorbic acid than fresh okra. Fresh okra fruits, on the other hand, had higher content of the colour pigments and were more viscous than all dehydrated products. Samples blanched prior to dehydration retained more of the colour components but were less viscous than unblanched samples. Vacuum dehydrated samples retained more ascorbic acid, colour pigment and mucilage at each of the dehydration temperatures than those from a hot air oven. High dehydration temperatures had negative effect on the colour, ascorbic acid and viscosity of okra. PMID:9713583

  3. Impact of Sucrose Concentrations on in vitro Pollen Germination of Okra, Hibiscus esculentus

    Mohammed Jurial Baloch

    2001-01-01

    Full Text Available Exogenous sugars, especially sucrose is very essential for providing osmotic environment and nutrition to in vitro pollen grain germination. Pollen bursting is frequently observed in artificial medium lacking suitable sucrose concentration. Four sucrose concentrations 10, 20, 30 and 40% were tried for okra pollen germination. At 10% sucrose,majority of pollens brusted; however, at 20% sucrose, 80% ofpollen grains germinated by producing tube lengths in the range of 3000 to 4000 μm. At 30%, the pollen germination% and tube length declined by about more thantwice against 20% sucrose level. At 40% concentrations, pollen grains` germination and tube length reduced remarkably by giving only500 to 700 μm pollen tubes. Not only tube length retarded at these high concentrations but pollentubes also changed to more thickened and shortened structures.It was therefore observed that 20% sucrose is an appropriate concentration for in vitro okra pollen germination.

  4. Efferent limb of gastrojejunostomy obstruction by a whole okra phytobezoar: Case report and brief review

    Thant Zin

    2012-01-01

    Full Text Available A phytobezoar is one of the intraluminal causes of gastric outlet obstruction, especially in patients with previous gastric surgery and/or gastric motility disorders. Before the proton pump inhibitor era, vagotomy, pyloroplasty, gastrectomy and gastrojejunostomy were commonly performed procedures in peptic ulcer patients. One of the sequelae of gastrojejunostomy is phytobezoar formation. However, a bezoar causing gastric outlet obstruction is rare even with giant gastric bezoars. We report a rare case of gastric outlet obstruction due to a phytobezoar obstructing the efferent limb of the gastrojejunostomy site. This phytobezoar which consisted of a whole piece of okra (lady finger vegetable was successfully removed by endoscopic snare. To the best of our knowledge, this is the first case of okra bezoar-related gastrojejunostomy efferent limb obstruction reported in the literature.

  5. Use of Biocontrol Fungi with Carbofuran in the Control Of Root Knot Nematodes in Okra

    M. J. Zaki

    1998-01-01

    Full Text Available Use of biocontrol fungi viz, Verticillium chlamydosporium, Paecilomyces lilacinus and Talaromyces flavus alone or mixed with carbofuran significantly (P<0.05 reduced root knot indices on okra plants as compared to control. Maximum reduction in glass formation was observed in the treatments where carbofuran was used either with V. chlamydosporium or P. lilacinus. Greater increase in fresh weight of shoot was observed where P. lilacinus was used with carbofuran followed by the use of P. lilacinus alone.

  6. Genetics of the Radiation-Induced Yellow Vein Mosaic Disease Resistance Mutation in Okra

    Yellow vein mosaic disease (YVMD) is one of the major diseases affecting okra production in Thailand. YVMD-resistant B4610 mutant was generated through gamma irradiation of the Okura variety of okra. In an attempt to develop a DNA marker for YVMD-resistance, a BC1F1 and an F2 mapping population were generated from the cross between B4610 and Pichit 03, a YVMD-susceptible variety. The populations were naturally inoculated with YVMD virus in the field at Pichit Horticultural Research Center, Pichit province, where the disease is widespread. Analysis of F1 and F2 progeny revealed the semi-dominant nature of the resistance which appeared to be caused by a single-locus mutation. MFLP fingerprintings of the F2 and the BC1F1 populations revealed a DNA fragment that is potentially linked to the mutation. In addition to the visual assessment of YVMD, a PCR method was developed for the assay of the presence of YVMD virus in leaf tissues. Sequencing of the amplified DNA fragments confirmed the presence of okra YVMD virus in the infected leaf tissues in susceptible plants. (author)

  7. Genetics of the radiation-induced yellow vein mosaic disease resistance mutation in okra

    The yellow vein mosaic disease (YVMD) is one of the major diseases affecting okra production in Thailand. YVMD-resistant B4610 mutant was generated through gamma irradiation of the Okura variety of okra. In an attempt to develop a DNA marker for YVMD-resistance, a BC1F1 and an F2 mapping population were generated from the cross between B4610 and Pichit 03, a YVMD-susceptible variety. The populations were naturally inoculated with YVMD virus in the field at Pichit Horticultural Research Center, Pichit province, where the disease is widespread. Analysis of F1 and F2 progeny revealed the semi-dominant nature of the resistance which appeared to be caused by a single-locus mutation. AFLP and MFLP fingerprintings of the F2 and the BC1F1 population revealed DNA fragments that are potentially linked to the mutation. In addition to the visual assessment of YVMD, a PCR method was developed for the assay of the presence of YVMD virus in leaf tissues. Sequencing of the amplified DNA fragments confirmed the presence of okra YVMD virus in the infected leaf tissues in susceptible plants. (author)

  8. Comparative Effect of Insecticide and Blue Polythene Hanging on the Incidence and Severity of Okra Mosaic in the Growth of Okra Plant

    Md. Fazlul Haque

    2002-01-01

    Full Text Available A field study was conducted to compare the efficacy of blue polythene hanging over the plant and spraying insecticide in reducing incidence and severity of okra mosaic under natural inoculated condition. Size of blue polythene strip was 0.04x3.5 m2 and six polythene strips were used per plot and the recommended dose of insecticide was 454 ml per acre. Blue polythene strips and insecticide reduced the infection of okra mosaic virus. The number of disease infected plants per plot and mosaic infected leaves per plant were the lowest and number of leaves per infected plant and plant height was the highest in the plots, where blue polythene strips were hanged and insecticide was sprayed followed by the spraying of insecticide and hanging of blue polythene strips. The number of disease infected plants per plot and mosaic infected leaves per plant were the highest and number of leaves per infected plant and plant height was the lowest in the control plots.

  9. Abelmoschus esculentus fractions potently inhibited the pathogenic targets associated with diabetic renal epithelial to mesenchymal transition.

    Peng, Chiung-Huei; Chyau, Charng-Cherng; Wang, Chau-Jong; Lin, Huei-Ting; Huang, Chien-Ning; Ker, Yaw-Bee

    2016-02-17

    Although Abelmoschus esculentus (AE) is known for anti-hyperglycemia, few reports have addressed its target. Our recent studies have focused on diabetic renal epithelial to mesenchymal transition (EMT), which plays a critical role in fibrosis that accompanies increasing vimentin and suggested signals DPP-4/AT-1/TGF-?1. This study aimed to investigate whether AE is useful for preventing diabetic renal EMT. We used a succession of extractions and obtained the corresponding fractions F1-F5, each with its own individual properties: F1 inhibits high glucose-stimulated vimentin, AT-1, TGF-?1, and DPP-4, and recovers E-cadherin in tubular cells; F2 decreases high glucose-induced vimentin, AT-1 and DPP-4; F3-F5 do not reduce the expression of vimentin. Chemical analysis revealed that F1 is rich of flavonoid glycosides especially quercetin glucosides, and pentacyclic triterpene ester. F2 contains a large amount of carbohydrates and polysaccharides composed of uronic acid, galactose, glucose, myo-inositol etc. In conclusion, AE has the potential to serve as an adjuvant for diabetic nephropathy, with F1 and F2 especially deserving further investigation and development. PMID:26787242

  10. Identification and characterization of a cytotoxic polysaccharide from the flower of Abelmoschus manihot.

    Zheng, Xiao; Liu, Zhihui; Li, Shuang; Wang, Liling; Lv, Jiajia; Li, Junsheng; Ma, Xingmiao; Fan, Ling; Qian, Fang

    2016-01-01

    A low molecular weight polysaccharide was identified from the flower of Abelmoschus manihot (Linn.) Medicus, a popular herbal medicine also known as Flos A. manihot. The polysaccharide, AMPS-a, was obtained from the ethanol-extracted debris of Flos A. manihot by successive purification through DEAE-cellulose-52 and Sephadex G-100 column. AMPS-a has an estimated molecular weight of 8.8×10(3)Da and is mainly composed of β-d-glucose, α-d-mannose, α-d-galactose and α-l-fucose at a molar ratio of 1.00:0.91:2.14:1.09. The preliminary structural features were studied by hydrolysis-methylation analysis, GC-MS, FT-IR and (1)H and (13)C NMR spectroscopy. AMPS-a contains a backbone composed of repeating units of →6)α-d-Galp-(1→6)α-d-Manp-(1→6)α-d-Galp-(1→ with β-d-Glcp (1→3)α-Fucp-(1→ branching at O-3 of mannose. AMPS-a exhibited potent inhibitory effects on the proliferation of hepatic (SMMC-7721, HepG2) and gastric (MGC-803, MKN-45) cancer cells in vitro, and the sugar branches were indispensible for the cytotoxicity. Our work contributes to the first report of a novel cytotoxic polysaccharide from Flos A. manihot that may provide a potential source of anti-tumor agent. PMID:26478094

  11. Aibika (Abelmoschus manihot L.): Genetic variation, morphology and relationships to micronutrient composition.

    Rubiang-Yalambing, Lydia; Arcot, Jayashree; Greenfield, Heather; Holford, Paul

    2016-02-15

    Aibika (Abelmoschus manihot L.) is believed to be a good source of micronutrients. However, although many varieties of aibika are commonly consumed in Papua New Guinea, their micronutrient content is unknown. Therefore, the mineral (Ca, Fe, K, Mg, Mn, Na, Zn & Cu), folate composition and the genetic variation of 23 aibika accessions from the collection at the National Agricultural Research Institute were studied over a 3year period to provide data for nutritional studies and to inform breeding programs. The data showed that aibika is, potentially, a crop of high nutritional value with the potential to boost the micronutrient status of local PNG communities. However, there were substantial differences in the micronutrient concentrations of the accessions from year to year and accessions that had the highest concentration of a particular mineral in 1year did not have the high concentrations in other years. Clusters determined using unweighted pair group method with arithmetic mean analysis (UPGMA) of the micronutrient contents differed in each of the 3years. Genetic analysis made using random amplification of polymorphic DNA and directed amplification of mini satellite region DNA placed the accessions into five groups. There was no correlation between these groups and leaf morphology, nor were there correlations with the clusters determined from the UPGMA analyses. There appears to be considerable interaction between genotype and environmental factors determining micronutrient composition and environmental factors may play a greater role than genotype in influencing micronutrient composition. PMID:26433288

  12. Removal of Anthracene and Fluoranthene by Waxy Corn, Long Bean and Okra in Lead-Contaminated Soil.

    Somtrakoon, Khanitta; Chouychai, Waraporn; Lee, Hung

    2015-09-01

    The ability of waxy corn, long bean and okra to remove two polycyclic aromatic hydrocarbons (PAHs) from soil containing 0.63 mg Pb kg(-1) dry soil was assessed. The presence of Pb did not reduce the ability of these plants to remove the PAHs from soil. About 49 % of anthracene and 77 % of fluoranthene were removed from Pb-spiked or non-spiked soil, respectively, after 30 days. Among the plants, okra was the most efficient at removing anthracene and fluoranthene in the presence or absence of Pb in soil after 30 days. Pb did not affect fluoranthene removal, but stimulated the removal of anthracene, by long bean, waxy corn and okra. However, growth of long bean and waxy corn was poor in Pb-spiked soil and waxy corn plants died around 22 days after transplantation. The results show some promise in using plants to remove PAHs from soil which is also co-contaminated with Pb. PMID:26149081

  13. Some physicochemical properties of acetaminophen pediatric suspensions formulated with okra gums obtained from different extraction processes as suspending agent

    Ikoni Ogaji

    2011-01-01

    Full Text Available The purpose of this work was to evaluate the effect of the extraction process and the potential of okra gum as a suspending agent in pharmaceutical oral formulations containing acetaminophen as a model drug. Clarified mucilage of dried okra was either extracted directly with ethanol 96% (F1 or was first treated with base (F2, acid (F3 or heating in the presence of salt (F4 before extraction with ethanol 96%. The samples were used at 0.5% w/v as suspending agents in acetaminophen acetaminophen suspension to deliver 125 mg/5 mL acetaminophen. A binary mixture of F2 and F4 (1:1 was also used. Similar suspensions of acetaminophen containing either hydroxymethylpropylcellulose (HPMC or tragacanth gum (TRAGA were produced. Some physicochemical properties of the formulations were evaluated. The rheological properties of acetaminophen-containing treated okra gums (F2-F5 were generally similar. Changes in viscosity with storage were slower in the F2-F5 formulations as compared with F1. Particle size and particle size distribution were different for all formulations, and hysteresis was a function of time and the suspending agent used. The re-dispersion time of the formulations with treated okra gums was generally shorter than that observed with the untreated okra gum. The use of a binary mixture of F2 and F4 resulted in different physicochemical properties from those of either F2 or F4. The physicochemical properties of the formulations were comparable to those with HPMC and TRAGA. It can thus be concluded that treating okra gum with acid, base or salt impacted better physicochemical properties on an acetaminophen pediatric suspension when they were used as suspending agents.

  14. VEGETATIVNI RAST LOKALNOG KULTIVARA OKRE (ABELMOSCHUS ESCULENTUS (L) MOENCH) POD UTJECAJEM GNOJA PERADI I ANORGANSKOG UMJETNOG GNOJIVA

    K. Olamide, Ogunsola; K.S., Olorunmaiye; O., Adeyemi Christiana

    2012-01-01

    Istraživano je djelovanje organskog i anorganskog umjetnog gnojiva na okru (Abelmoschus esculentus) (lokalni kultivar od 40 dana) u biološkom vrtu Sveučilišta IIorin u IIorinu u Nigeriji. Upotrijebljen je gnoj peradi kao organsko umjetno gnojivo dok se anorgansko umjetno gnojivo sastojalo od jednostavnog superfosfata, ureje i NPK 15:15:15. Dobiveni rezultati pokazuju da je primjena gnoja peradi poboljšala vegetativni rast u usporedbi s NPK 15:15:15 i urejom, jednostavnim superfosfatom i kontr...

  15. Response of young plants to mercury. [Pennisetum typhoideum, Medicago sativa L. var. Raska, Abelmoschus esculentus Moench. var. Pusa-savni

    Mhatre, G.N.; Chaphekar, S.B.

    1984-01-01

    Young individuals of three plant species viz., Pennisetum typhoideum Stapf. var. PHB-14, Medicago sativa L. var. Raska and Abelmoschus esculentus Moench. var. Pusa-savni, were tested for their response to Hg. All of them were found to be affected, Pennisetum being the most sensitive, followed by Medicago and Abelmoxchus in that order. The visible injury to leaves of plants, chlorophyll content and total dry matter was found to be affected in proportion to the metal doses administered. Measurement of Leaf Injury Index (LII) as a simple parameter of the susceptibility of the species is discussed.

  16. Novel extraction and application of okra gum as a film coating agent using theophylline as a model drug

    Ogaji, Ikoni J; Hoag, Stephen W.

    2014-01-01

    The purpose of this study was to investigate the effect of extraction and application of okra gum as an aqueous film coating agent. Powdered okra pods dispersed in demineralized water was heated at 80 ± 2oC for 30 minutes in the presence of sodium chloride. The filtrate was successively centrifuged at 4000 rpm for 30, 60, or 120 minutes and freeze dried. The samples were used as film former at different concentrations in aqueous film coating operations. Near infrared (nIR) absorption spectra,...

  17. Organophosphate Pesticide Residues in Okra and Brinjal Grown in Peri-Urban Environment of Big Cities of Punjab

    Peri urban farming system is main supplier of vegetables to urban population and organophosphate pesticides exposure due to consumption of okra and brinjal to urban population has been estimated in this study. Okra and brinjal samples (90 samples for each vegetable) were collected from peri-urban farming area of Faisalabad, Multan and Gujranwala and were analysed by GC-ECD for quantification of chlorpyrifos, profenophos and triazophos residues. Data were statistically analyzed to calculate, mean and standard deviation. Quantification of pesticides residues revealed that 85 (47.22 percentage) samples were contaminated and 15 (8.33 percentage of total samples and 17.64 percentage of contaminated) samples showed residues value higher than MRL'S. Out of 85 contaminated samples 27 (31.76 percentage) samples were contaminated with chlorpyrifos, 30 (35.29 percentage) samples with profenofos and 29 (34.11) were contaminated with triazophos. The mean concentration of chlorpyrifos in okra was 0.192 mg Kg-1 and brinjal 0.197 mg Kg-1. The mean concentration for profenofos in okra was 0.02 mg Kg-1 and brinjal 0.035 mg Kg-1. Similarly mean concentration for triazophos in okra was 0.009 mg Kg-1 and brinjal 0.01 mg Kg-1. Overall, 6.67 percentage samples of okra and 6.66 percentage samples of brinjal contaminated with chlorpyrifos exceeded EU MRL of 0.05 mg kg-1. Okra samples were found contaminated at frequency of 4.44 percentage and brinjal samples at frequency of 5.56 percentage with higher profenofos residues level than MRL of 0.05 mg kg-1. Approximately, 6.67 percentage samples of okra and 5.6 percentage samples of brinjal had profenofos residue level above MRL of 0.01 mg kg-1. This study may be helpful for building database regarding regional exposure of urban population and facilitate in estimation of possible risk to their health in our daily life. (author)

  18. Metabolite identification strategy of non-targeted metabolomics and its application for the identification of components in Chinese multicomponent medicine Abelmoschus manihot L.

    Guo, Jian-ming; Lu, Yu-wei; Shang, Er-xin; Li, Ting; Liu, Yang; Duan, Jin-ao; Qian, Da-wei; Tang, Yu-ping

    2015-05-15

    Identification of multicomponent in traditional Chinese medicine (TCM) is complex and time-consuming. The inspection of the full-scan mass chromatograms was usually performed manually, which is labor-intensive. It is difficult to distinguish low response signals from complex chemical background. Furthermore, this process is typically based on earlier knowledge of the chemical composition of TCM, and those molecules that have not been characterized earlier were thus ignored. In this paper, a strategy using UPLC-MS combined with pattern recognition analysis was developed to simplify and quicken the identification of multicomponent in Abelmoschus manihot (L.) Medik. First, complex signals obtained by UPLC-MS were processed using automated data mining algorithm and further processed with multivariate chemometric methods. Multicomponent in Abelmoschus manihot L. can be clearly displayed in S- and VIP-plot. Using this method, 320 peaks which present in Abelmoschus manihot L. were detected. In the next step, accurate mass spectra of the characteristic markers acquired by QTOF MS were used to estimate their elemental formulae and enable structure identification. By searching in METLIN database, 41 components were tentatively identified in Abelmoschus manihot L. Our results showed that UPLC-MS based-pattern recognition analysis approach can be used to quickly identify TCM multicomponent and for standardization of herbal preparations. PMID:25981925

  19. Persistence and risk assessment of emamectin benzoate residues on okra fruits and soil.

    Jyot, Gagan; Mandal, Kousik; Chahil, G S; Singh, Balwinder

    2014-08-01

    Emamectin benzoate, a synthetic derivative of abamectin, is found effective against fruit borer and jassid in okra crops. The present studies were carried out to study the dissipation pattern of emamectin benzoate on okra and to suggest a suitable waiting period for the safety of consumers. Following three applications of emamectin benzoate (Proclaim 5 SG) at 68.1 and 136.2 g a.i. ha-1, the average initial deposits of emamectin benzoate were observed to be 0.22 and 0.42mg kg-1, respectively. These residues dissipated below the limit of quantification (LOQ) of 0.05 mg kg-1 after 5 days at both the dosages. Soil samples collected after 15 days did not reveal the presence of emamectin benzoate at LOQ of 0.05 mg kg-1. Acceptable daily intake (ADI) of emamectin benzoate is 0.0005 mg kg-1 body weight day-1, which means an adult of 55 kg weight can safely tolerate an intake of 27.50 microg emamectin benzoate. Assuming an average consumption of 80 g okra fruit and multiplying it by average and maximum residues observed on 0 day at recommended dosage, the intake of emamectin benzoate comes out to be about 20 Itg and these values are quite safe in comparison to its ADI. These studies, therefore, suggest that the use of emamectin benzoate at the minimum effective dosages do not seem to pose any hazards to the consumers if a waiting period of 1 day is observed. PMID:24956765

  20. Treatment of chronic kidney disease using a traditional Chinese medicine, Flos Abelmoschus manihot (Linnaeus) Medicus (Malvaceae).

    Chen, Yizhi; Cai, Guangyan; Sun, Xuefeng; Chen, Xiangmei

    2016-02-01

    The flowers of Abelmoschus manihot (Linnaeus) Medicus (Malvaceae; Flos A. manihot) have been used in China for many centuries as a traditional Chinese medicine for the treatment of chronic kidney disease. The Huangkui capsule is a single-plant drug extracted from the dry corolla of Flos A. manihot that has been approved by China's State Food and Drug Administration for the treatment of chronic glomerulonephritis. The purpose of this paper is to review briefly some of the past experiences in rapid filtration and to present more fully a few facts brought out in recent studies. The primary chemical constituents of Flos A. manihot are flavonoids. In vivo, the flavonoids can be transformed into glucuronide-sulphate conjugates, which are the major metabolites of Flos A. manihot and could contribute to the renoprotective effects in vivo. Flos A. manihot can ameliorate proteinuria, podocyte apoptosis, glomerulosclerosis and mesangial proliferation. The renoprotective effects of Flos A. manihot are related to inhibition of caspase-3 and caspase-8 overexpression, reduction of the infiltration of ED1(+) and ED3(+) macrophages, downregulation of oxidative stress, inhibition of the p38 mitogen-activated protein kinase and serine/threonine kinase pathways and suppression of transforming growth factor-?1 and tumour necrosis factor-? expression. Recently, a multicentre randomized controlled trial demonstrated that Flos A. manihot was more effective than the angiotensin-receptor blocker losartan in reducing proteinuria in patients with primary glomerular disease. Because Flos A. manihot is generally preferred by Chinese patients and clinicians, high-quality trials to test the efficacy and safety of Flos A. manihot are urgently needed. PMID:26667396

  1. Method validation and dissipation dynamics of chlorfenapyr in squash and okra.

    Abdel Ghani, Sherif B; Abdallah, Osama I

    2016-03-01

    QuEChERS method combined with GC-IT-MS was developed and validated for the determination of chlorfenapyr residues in squash and okra matrices. Method accuracy, repeatability, linearity and specificity were investigated. Matrix effect was discussed. Determination coefficients (R(2)) were 0.9992 and 0.9987 in both matrices. LODs were 2.4 and 2.2μg/kg, while LOQs were 8.2 and 7.3μg/kg. Method accuracy ranged from 92.76% to 106.49%. Method precision RSDs were ⩽12.59%. A field trial to assess chlorfenapyr dissipation behavior was carried out. The developed method was employed in analyzing field samples. Dissipation behavior followed first order kinetics in both crops. Half-life values (t1/2) ranged from 0.2 to 6.58days with determination coefficient (R(2)) ranged from 0.78 to 0.96. The developed method was utilized for surveying chlorfenapyr residues in squash and okra samples collected from the market. Monitoring results are discussed. PMID:26471587

  2. Bioadhesive okra polymer based buccal patches as platform for controlled drug delivery.

    Kaur, Gurpreet; Singh, Deepinder; Brar, Vivekjot

    2014-09-01

    In the present investigation, polysaccharide from the Okra fruits (Hibiscus esculentus) was extracted, characterized and explored for its mucoadhesive potential. Mucoadhesive films of okra polymer (OP) were prepared by solvent casting method based on 3(2) factorial design. For these studies, OP (2.0%, 2.5%, 3.0%, w/v) and glycerol (plasticizer) (0.25%, 0.50%, 0.75%, v/v) were taken as independent variables while tensile strength, mucoadhesive strength, contact angle, swelling index and residence time as dependent variables. The developed films were evaluated for their physicochemical, mechanical and electrical properties. The formulated films were found to be smooth, flexible, and displayed adequate mucoadhesive and tensile strength. Their near neutral pH and negative hemolytic studies indicated their non-irritability and biocompatible nature with biological tissues. The formulation comprising of 3% OP and 0.5% glycerol (F8) was found to exhibit optimum mechanical properties. Further, optimized film was loaded with zolmitriptan (model drug) to determine its drug release profiles. In vitro and ex vivo drug release studies demonstrated a controlled release of zolmitriptan over a period of 8h in simulated salivary fluid (SSF) pH 6.8, with the correlation coefficient values indicating its non-Fickian kinetics. Thus, OP can be used as a promising biomaterial for controlled drug delivery. PMID:25036601

  3. Okra (Hibiscus esculentus) gum-alginate blend mucoadhesive beads for controlled glibenclamide release.

    Sinha, Priyanka; Ubaidulla, U; Nayak, Amit Kumar

    2015-01-01

    The utility of isolated okra (Hibiscus esculentus) gum (OG) was evaluated as a potential sustained drug release polymer-blends with sodium alginate in the development of controlled glibenclamide release ionically-gelled beads for oral use. OG was isolated from okra fruits and its solubility, pH, viscosity and moisture content were studied. Glibenclamide-loaded OG-alginate blend beads were prepared using CaCl2 as cross-linking agent through ionic-gelation technique. These ionically gelled beads showed drug entrapment efficiency of 64.19 ± 2.02 to 91.86 ± 3.24%. The bead sizes were within 1.12 ± 0.11 to 1.28 ± 0.15 mm. These glibenclamide-loaded OG-alginate blend beads exhibited sustained in vitro drug release over a prolonged period of 8 h. The in vitro drug release from these OG-alginate beads were followed controlled-release (zero-order) pattern with super case-II transport mechanism. The beads were also characterized by SEM and FTIR. The swelling and degradation of these beads was influenced by the pH of the test medium. These beads also exhibited good mucoadhesivity with goat intestinal mucosa. PMID:25312603

  4. Inheritance of Yellow Vein Mosaic Disease Resistance, a Mutant Phenotype from Gamma Radiation Mutagenesis in Okra

    Full text: The yellow vein mosaic disease (YVMD) is one of the major diseases affecting export-grade okra production in Thailand. YVMD-resistant B4610 mutant was generated through gamma irradiation of the Okura variety of okra. This study aimed at determining the inheritance of YVMD resistance from B4610 through genetic analysis. Segregation analysis of the F2 and BC1F1 progeny from the cross between B4610 and Pichit 03, a susceptible variety, indicated that YVMD resistance phenotype resulted from a single locus mutation (p > 0.05). In addition, a polymerase chain reaction (PCR) assay was developed to aid in the assignment of YVMD resistance phenotype. Combinations of primers AV1F1 or AV1F2 with AV1R1 successfully amplified a DNA fragment from infected susceptible individuals but not from resistant individuals. DNA sequencing of the PCR product confirmed the identity of AV1 gene belonging to the yellow vein mosaic virus

  5. Effects of management practices on nematode and fungi populations and okra yield.

    Johnson, A W; Sumner, D R; Jaworski, C A; Chalfant, R B

    1977-04-01

    Okra was grown in field plots of Tifton loamy sand naturally infested with the nematodes Meloidogyne incognita and Criconemoides ornalus and the pathogenic fungi Fusarium oxysporum, F. solani, F. roseum, and Pythium spp. Plots were treated with various soil pesticides and left exposed or covered with biodegradable paper film mulch under trickle irrigation. Soil was assayed for nematodes and fungi, and plant roots were examined for root-rot and insect damage. Fewer nematodes and fungi generally were recovered from soil treated with DD-MENCS (with and without film mulch) or methyl bromide-chloropicrin (2:1) (MBC) and film mulch than from nontreated soil. Funfigation with DD-MENCS or MBC suppressed populations of M. incognita, C. ornatus, F. oxysporum, F. solani, F. roseum, and Pythium spp. Ethoprop (alone or combined with other pesticides), sodium azide, and chloroneb were less effective than DD-MENCS and MBC. Plant growth anti yield were greatest when nematodes and pathogenic fungi were controlled. Yield was increased 3-fold by DD-MENCS + film mulch or MBC + film mulch in comparison with the average yield of okra produced in Georgia. The root-knot nematode-Fusarium wilt complex was most severe in nonfuntigated soil. PMID:19305580

  6. Novel extraction and application of okra gum as a film coating agent using theophylline as a model drug

    Ikoni J. Ogaji

    2014-01-01

    Full Text Available The purpose of this study was to investigate the effect of extraction and application of okra gum as an aqueous film coating agent. Powdered okra pods dispersed in demineralized water was heated at 80 ± 2 o C for 30 minutes in the presence of sodium chloride. The filtrate was successively centrifuged at 4000 rpm for 30, 60, or 120 minutes and freeze dried. The samples were used as film former at different concentrations in aqueous film coating operations. Near infrared (nIR absorption spectra, photomicrographs, and some physicochemical properties of the coated tablets were evaluated. The okra gum samples had different nIR spectra and possessed good processing and application quality due to relatively low viscosity. A six-fold concentration of this gum from the novel extraction yielded glossy theophylline tablets within a short time. A t (18 = 2.895, P < 0.005, t critical = 1.734 were obtained for the independent analysis of the hardness of core and coated theophylline tablets. A 3.0% concentration of the okra samples at a flow rate of 3 ml/min for 100 minutes showed that F = 3.798, DF = 29, P < 0.035, F critical = 3.354 in tablet hardness among samples and F = 15.632, DF = 29, P < 0.0001, F critical = 2.152 were obtained on film thickness among tablet samples during the coating and drying operation. Novel extraction process enhanced the film coating potential of okra gum by delivering more solids on the substrate at a shorter time with improved operation efficiency.

  7. Systematic Implications of Seed Coat Morphology in Malvaceae

    Salah M. I. El Naggar

    2001-01-01

    Full Text Available Seed morphological characters and seed coat sculpture of 14 species of Malvaceae were examined to assess systematic implications of seed coat sculpture. These taxa are Malva aegyptia L., M. parviflora L., M. neglecta Wallr., M. nicaeensis All., M. sylvestris L., Lavatera cretica L., Alcea rosea All., Sida alba L., Abutilon theophrasti Medicus, A. pannosum (G. Forster Schlecht., Abelmoschus esculentus (L. Moench. Hibiscus sabdariffa L., H. trionum L., and Gossypium barbadense (L.. Seed shape, size and colour as well as outer epidermal cell patterns as seen by SEM, anticlinal cell boundaries and periclinal cell walls sculpture allow the recognition of two distinct groups among studied taxa. Seed coat features indicated both intra-group and inter-group relationships. Recognized groupings do not correspond well with the classical treatments of the Malvaceae, rather they suggest restructuring of tribal and generic taxonomy in the family. One important conclusion of this study is the inclusion of Lavatera cretica L. in the genus Malva as M. linnaei, M. F. Ray.

  8. La culture du gombo (Abelmoschus spp) Legume fruit tropical avec reference speciale a la Cote d'Ivoire

    Siemonsma, J.S.

    1982-01-01

    The data for this study have been gathered in Ivory Coast during the period 1977-1980.The first purpose of the study was a description of the traditional okra cultivation and, in particular, the evaluation of the local planting material in order to determine its potential for crop improvement.The second purpose of the programme was a study of the agronomic limitations in the case of intensive cultivation and in what ways such problems might be solved.The inventories of vegetables offered for ...

  9. GROWTH AND YIELD OF OKRA AS INFLUENCED BY LIVE MULCH IN SAMARU, ZARIA

    I. L HAMMA

    2012-06-01

    Full Text Available Two field experiments were conducted at the Teaching and Research Farm of theInstitute for Agricultural Research; Ahmadu Bello University Zaria located on latitude11011’N, longitude 7038’E and 686m above sea level in the Northern Guinea SavannahEcological Zone of Nigeria in 2010 and 2011 cropping seasons. The objective of thestudy was to compare the yield of okra grown under different weed managementstrategies. The experiment consisted of four treatments namely: control, cucumber,watermelon and pumpkin. The experiment was laid out in a completely randomised blockdesign (RCBD with three replications. From the results so far obtained, pumpkinsignificantly gave higher mean values in both growth and yield characters measured,whereas the control treatment, significantly gave lower mean values of both growth andyield parameters throughout the period of assessment.

  10. Residue analysis of fipronil and difenoconazole in okra by liquid chromatography tandem mass spectrometry and their food safety evaluation.

    Hingmire, Sandip; Oulkar, Dasharath P; Utture, Sagar C; Ahammed Shabeer, T P; Banerjee, Kaushik

    2015-06-01

    A liquid chromatography tandem mass spectrometry (LC-MS/MS) based method is reported for simultaneous analysis of fipronil (plus its metabolites) and difenoconazole residues in okra. The sample preparation method involving extraction with ethyl acetate provided 80-107% recoveries for both the pesticides with precision RSD within 4-17% estimated at the limits of quantification (LOQ, fipronil=1ngg(-1), difenoconazole=5ngg(-1)) and higher fortification levels. In field, both the pesticides dissipated with half-life of 2.5days. The estimated pre-harvest intervals (PHI) for fipronil and difenoconazole were 15 and 19.5days, and 4 and 6.5days at single and double dose of field applications, respectively. Decontamination of incurred residues by washing and different cooking treatments was quite efficient in minimizing the residue load of both the chemicals. Okra samples harvested after the estimated PHIs were found safe for human consumption. PMID:25624217

  11. Identification of DNA Fragments that Showed Linkage to the Radiation-induced Yellow Vein Mosaic Disease Resistance Mutation in Okra

    Full text: The yellow vein mosaic disease resistant mutant of okra was crossed to Pichit 03, a susceptible variety. Their progeny showed prolonged resistance when compared with Pichit 03. DNA fingerprints of F2 and BC1F1 individuals from the cross indicated that most DNA bands did not segregate with either the resistance or the susceptible characteristics. Nonetheless, polymorphic DNA bands could be identified between the mutant and Okura, the parental variety

  12. Effect of Culture Filtrate of Fungi in the Control of Meloidogyne javacnica, Root Knot Nematodes on Okra and Broad Bean

    Amer-Zareen

    1999-01-01

    Full Text Available Fungal cell free filtrates were used in two different doses, enhanced plant growth and root knot nematodes infection was reduced where high doses of filtrate (100% concentration were applied, in all test fungal filtrates. Culture filtrates of Paecilomyces lilacinus and Verticillium chlamydosporium at 100 percent concentration showed significant reduction in Meloidogyne javanica root knot infection on okra and broad bean as compared to Trichoderma harzianum, T. koningii, T. viride, Aspergillus restrictus and Aspergillus sp., which found less effective.

  13. Application of the exploratory analysis of data in the geographical discrimination of okra of Rio Grande do Norte and Pernambuco

    Francisco Santos Panero

    2009-11-01

    Full Text Available The contents of Cu, Zn, Na, Fe, K, Ca, Mn, Mg, PO43-, Cl- and SO42- were determined in samples of okra of the municipal districts of Caruaru and Vitória de Santo Antão, in Pernambuco, as well as in the municipal districts of Ceará-Mirim, Macaíba and Extremoz in the state of Rio Grande do Norte. The objective of this work is the application of two methods of  exploratory analysis of data: Principal Component Analysis - PCA and Hierarquical Cluster Analysis - HCA in the geographical discrimination of okra originating in the states of Rio Grande do Norte and Pernambuco. The results showed that Cl- and Na were the main elements for the differentiation of the samples of Rio Grande do Norte and, the samples of Pernambuco presented the largest amount of Fe, Cu, Mn, Mg, Ca, Zn, K, PO43-, and SO42-. Boths the methods of exploratory analysis of data investigated are efficient for geographical discrimination of okra originating in Rio Grande do Norte and Pernambuco.

  14. Identification of a disease complex involving a novel monopartite begomovirus with beta- and alphasatellites associated with okra leaf curl disease in Oman.

    Akhtar, Sohail; Khan, Akhtar J; Singh, Achuit S; Briddon, Rob W

    2014-05-01

    Okra leaf curl disease (OLCD) is an important viral disease of okra in tropical and subtropical areas. The disease is caused by begomovirus-satellite complexes. A begomovirus and associated betasatellite and alphasatellite were identified in symptomatic okra plants from Barka, in the Al-Batinah region of Oman. Analysis of the begomovirus sequences showed them to represent a new begomovirus most closely related to cotton leaf curl Gezira virus (CLCuGeV), a begomovirus of African origin. The sequences showed less than 85 % nucleotide sequence identity to CLCuGeV isolates. The name okra leaf curl Oman virus (OLCOMV) is proposed for the new virus. Further analysis revealed that the OLCOMV is a recombinant begomovirus that evolved by the recombination of CLCuGeV isolates with tomato yellow leaf curl virus-Oman (TYLCV-OM). An alpha- and a betasatellite were also identified from the same plant sample, which were also unique when compared to sequences available in the databases. However, although the betasatellite appeared to be of African origin, the alphasatellite was most closely related to alphasatellites originating from South Asia. This is the first report of a begomovirus-satellite complex infecting okra in Oman. PMID:24287711

  15. Degradation analysis of some synthetic and bio-insecticides sprayed on okra crop using HPLC

    This study aimed to find out the degradation of three conventional and two bio-insecticides sprayed on okra crop. Imidacloprid, Endosulfan and Profenofos were selected as convectional and biosal and spinosad as bioinsecticide. The insecticides were sprayed at the rates of 49.4, 642.2, 988, 35.5 and 158 g. a. i. ha/sup -1/ respectively. The insecticide residues were analyzed in the leaf and fruit after 0, 1, 3 and 7 days using high performance liquid chromatography. First order degradation kinetics was fitted on this data and degradation rate constants and half life were calculated. Conventional insecticides were found to be more persistent in the crop (Average half life: 1.95, 2.42 and 1.57 days for imidacloprid, endosulfan and profenofos respectively) than bioinsecticides (Average half life 1.25 and 0.27 days for spinosad and biosal respectively). Residues of all tested insecticides were compared with codex and EU MRLs and found both the bio-insecticides treated crops safe for human consumption even after few hours of spray. Endosulfan and profenofos treated crops were not found to be fit for consumption even after 7 days of application. Imidacloprid being biorational (low risk) was also safe for consumption on the next day of application. (author)

  16. Effect of okra cell wall and polysaccharide on physical properties and stability of ice cream.

    Yuennan, Pilapa; Sajjaanantakul, Tanaboon; Goff, H Douglas

    2014-08-01

    Stabilizers are used in ice cream to increase mix viscosity, promote smooth texture, and improve frozen stability. In this study, the effects of varying concentrations (0.00%, 0.15%, 0.30%, and 0.45%) of okra cell wall (OKW) and its corresponding water-soluble polysaccharide (OKP) on the physical characteristics of ice cream were determined. Ice cream mix viscosity was measured as well as overrun, meltdown, and consumer acceptability. Ice recrystallization was determined after ice cream was subjected to temperature cycling in the range of -10 to -20 °C for 10 cycles. Mix viscosity increased significantly as the concentrations of OKW and OKP increased. The addition of either OKW or OKP at 0.15% to 0.45% significantly improved the melting resistance of ice cream. OKW and OKP at 0.15% did not affect sensory perception score for flavor, texture, and overall liking of the ice cream. OKW and OKP (0.15%) reduced ice crystal growth to 107% and 87%, respectively, as compared to 132% for the control (0.00%). Thus, our results suggested the potential use of OKW and OKP at 0.15% as a stabilizer to control ice cream quality and retard ice recrystallization. OKP, however, at 0.15% exhibited greater effect on viscosity increase and on ice recrystallization inhibition than OKW. PMID:25040189

  17. Potential of soil amendments (Biochar and Gypsum) in increasing water use efficiency of Abelmoschus esculentus L. Moench

    Batool, Aniqa; Taj, Samia; Rashid, Audil; Khalid, Azeem; Qadeer, Samia; Saleem, Aansa R.; Ghufran, Muhammad A.

    2015-01-01

    Water being an essential component for plant growth and development, its scarcity poses serious threat to crops around the world. Climate changes and global warming are increasing the temperature of earth hence becoming an ultimate cause of water scarcity. It is need of the day to use potential soil amendments that could increase the plants’ resistance under such situations. Biochar and gypsum were used in the present study to improve the water use efficiency (WUE) and growth of Abelmoschus esculentus L. Moench (Lady’s Finger). A 6 weeks experiment was conducted under greenhouse conditions. Stress treatments were applied after 30 days of sowing. Plant height, leaf area, photosynthesis, transpiration rate (Tr), stomatal conductance and WUE were determined weekly under stressed [60% field capacity (F.C.)] and non-stressed (100% F.C.) conditions. Stomatal conductance and Tr decreased and reached near to zero in stressed plants. Stressed plants also showed resistance to water stress upto 5 weeks and gradually perished at sixth week. On the other hand, WUE improved in stressed plants containing biochar and gypsum as compared to untreated plants. Biochar alone is a better strategy to promote plant growth and WUE specifically of A. esculentus, compared to its application in combination with gypsum. PMID:26442046

  18. Potential of soil amendments (Biochar and Gypsum) in increasing water use efficiency of Abelmoschus esculentus L. Moench.

    Batool, Aniqa; Taj, Samia; Rashid, Audil; Khalid, Azeem; Qadeer, Samia; Saleem, Aansa R; Ghufran, Muhammad A

    2015-01-01

    Water being an essential component for plant growth and development, its scarcity poses serious threat to crops around the world. Climate changes and global warming are increasing the temperature of earth hence becoming an ultimate cause of water scarcity. It is need of the day to use potential soil amendments that could increase the plants' resistance under such situations. Biochar and gypsum were used in the present study to improve the water use efficiency (WUE) and growth of Abelmoschus esculentus L. Moench (Lady's Finger). A 6 weeks experiment was conducted under greenhouse conditions. Stress treatments were applied after 30 days of sowing. Plant height, leaf area, photosynthesis, transpiration rate (Tr), stomatal conductance and WUE were determined weekly under stressed [60% field capacity (F.C.)] and non-stressed (100% F.C.) conditions. Stomatal conductance and Tr decreased and reached near to zero in stressed plants. Stressed plants also showed resistance to water stress upto 5 weeks and gradually perished at sixth week. On the other hand, WUE improved in stressed plants containing biochar and gypsum as compared to untreated plants. Biochar alone is a better strategy to promote plant growth and WUE specifically of A. esculentus, compared to its application in combination with gypsum. PMID:26442046

  19. Sensory evaluation ratings and melting characteristics show that okra gum is an acceptable milk-fat ingredient substitute in chocolate frozen dairy dessert.

    Romanchik-Cerpovicz, Joelle E; Costantino, Amanda C; Gunn, Laura H

    2006-04-01

    Reducing dietary fat intake may lower the risk of developing coronary heart disease. This study examined the feasibility of substituting okra gum for 25%, 50%, 75%, or 100% milk fat in frozen chocolate dairy dessert. Fifty-six consumers evaluated the frozen dairy desserts using a hedonic scale. Consumers rated color, smell, texture, flavor, aftertaste, and overall acceptability characteristics of all products as acceptable. All ratings were similar among the products except for the aftertaste rating, which was significantly lower for chocolate frozen dairy dessert containing 100% milk-fat replacement with okra gum compared with the control (0% milk-fat replacement) (Pchocolate frozen dairy dessert. PMID:16567157

  20. Effects of Kraal Manure ApplicationRates on Growth and Yield of Wild Okra (Corchorus olitorius L in a Sub-tropical Environment

    Michael T. Masarirambi

    2012-01-01

    Full Text Available Wild okra (Corchorus olitorius L is an important indigenous vegetable in Swaziland. Although the crop is a popular vegetable among rural communities, not much has been done to produce organic fertilizer application recommendations for its production. The purpose of this research was to investigate the effects of kraal manure application rates on growth and yield of wild okra. Kraal manure was applied at varying rates of 20, 40 and 60 tons/ha in a Randomized Complete Block Design (RCBD where 2.3.2 (22 an inorganic fertilizer was used as a control, and was applied at the rate of 150 kg/ha. For every increase in the application level of kraal manure there were significant (p<0.05 increases in all the growth parameters that were measured. There were also significant (p<0.05 differences of fresh mass and dry mass yield of wild okra. There was an increase in fresh and dry mass yield with every increase in application level of kraal manure while the least fresh and dry mass was recorded in plants provided with 2.3.2 (22. Kraal manure applied at 60 ton/ha gave the highest yield of wild okra under the conditions of this experiment.

  1. Formulation of Okra-natural Mucilage as Drag Reducing Agent in Different Size of Galvanized Iron Pipes in Turbulent Water Flowing System

    R.B.M. Yunus

    2010-01-01

    Full Text Available The problem of pumping power losses in pipelines carrying liquids and flowing in turbulent mode is one of the major challenges in the power saving field. Pumping power saving by the addition of minute quantities of additives to the main flow was applied in the present study. Natural drag reducing agent was prepared and extracted from okra fruit and it was tested in a closed loop of turbulence water flowing system. Flow tests were conducted using water as the carrying liquid. The experimental work starts by pumping water from reservoir tank that had mixed with mucilage was pumped with six different flow rates in two different pipe diameters (0.015, 0.025 m ID. The types of pipe used are galvanized iron pipe. The testing length of this flow system is 1.5 m. The aim of this study is to formulate and to test the efficiency of okra-natural mucilage as drag reducer agent on transport of water in pipes; different concentrations of mucilage (100, 300, 500, 700 and 1000 ppm were used. Six different flow rates were used in the purpose to investigate the flow rates effect. The efficiency of mucilage was tested using clear water. The results shows that, percentage drag reduction (Dr% increases by increasing the concentration of okra-natural mucilage. Maximum Dr% of 71% was obtained using 1000 ppm of okra-natural mucilage in water flow system.

  2. Genetic architecture of fruit yield and its contributing quantitative traits in Abelmoschus esculentus (L. Moench

    Khanorkar S.M. and K. B. Kathiria

    2010-07-01

    Full Text Available The genetic architecture of fruit yield and its related quantitative traits viz., days to first flowering, days to first picking, plantheight, primary branches per plant, stem girth, fruit length, fruit girth, fruit weight, fruits per plant and fruit yield per plantstudied through generation mean analysis using six basic generations (P1, P2, F1, F2, BC1 and BC2 of six crosses HRB-55 xAOL-05-4, VRO-5 x Red Long, VRO-6 x AOL-05-3, GO-2 x AOL-04-3, Arka Anamika x AOL-03-1 and Parbhani Kranti xAOL-03-6 in two environments (E1 and E2. Among the generations within the crosses, the sum of squares showed significantdifferences for all characters. Fixable type of gene effects viz., additive [d] and additive x additive [i] found significant fordays to first flowering in crosses HRB-55 x AOL-05-4 (E1, VRO-5 x Red Long (E2 and GO-2 x AOL-04-3 (E1. The similarkind of gene effects also observed for days to first picking (E1 and fruit weight (E2 in the cross GO-2 x AOL-04-3. In anothercase, dominance [h] and dominance x dominanace [l] which non-fixable gene effects were significant for days to firstflowering in crosses VRO-5 x Red Long (E2 and GO-2 x AOL-04-3 (E1; stem girth in VRO-6 x AOL-05-3 (E1 and fruitlength in Arka Anamika x AOL-03-1 (E1. Duplicate type of epistasis observed for days to first flowering in crosses, VRO-5 xRed Long (E2 and GO-2 x AOL-04-3 (E1; stem girth in VRO-6 x AOL-05-3 (E1 and fruit length in cross Arka Anamika xAOL-03-1 (E1. Results revealed additive and additive x additive types of fixable gene effects for days to first flowering anddays to first picking as well as fruit yield and its contributing traits in some cross-environment combinations. This suggestssimple selection or a single seed descent method could help for improvement of these traits. The results on epistatic geneeffects for fruit yield and its contributing component traits in different cross-environment combinations suggests that recurrentselection, bi-parental mating and inter se mating between desirable segregants followed by selection or multiple crosses offergood promising methods.

  3. Comparative sorptive removal of direct red-28 dye from water using acid, base and 2-propanol treated okra stem powder

    Summary: Use of agro-waste material for adsorption is studied now-a-days extensively. But the main problem is their low adsorption capacity as compared to synthetic adsorbents. So, in the following study, adsorption capacity of Okra stem powder had been investigated after chemical modification by acid, base and 2-propanol for adsorption of Direct Red-28 dye from water in batch mode. The results indicated that as compared to untreated biosorbent , acid treated and 2-propanol treated biosorbents had more adsorption capacity, i.e. 1.86, 2.74 and 6.61 mg.g/sub -1/ correspondingly, whereas base treated biosorbent had lower adsorption capacity for this anionic dye, i.e.1.02 mg.g/sub -1/. (author)

  4. Integrated effect of different N-fertilizer rates and bioslurry application on growth and N-use efficiency of okra (Hibiscus esculentus L.)

    Muhammad SHAHBAZ; AKHTAR, Muhammad Javed; Ahmed, Wazir; WAKEEL, Abdul

    2014-01-01

    Bioslurry obtained from biogas plants has the potential to reduce the use of expensive chemical fertilizers and increase yields. A field experiment was conducted to investigate the growth response and yield production of okra fertilized with various combinations of bioslurry and nitrogen fertilizer. The experiment was planned according to randomized complete block design with 3 replications. Bioslurry was analyzed for its composition and was applied at the rate of 600 kg ha-1 along with 50%, ...

  5. Begomovirus characterization, and development of phenotypic and DNA-based diagnostics for screening of okra genotype resistance against Bhendi yellow vein mosaic virus

    Venkataravanappa, V.; Lakshminarayana Reddy, C. N.; Krishna Reddy, M.

    2012-01-01

    The leaf sample from okra plants showing the yellow vein mosaic disease symptoms was collected in Karnataka state, India. The genome of the virus was amplified, cloned and sequenced. Sequence analysis revealed that the viral genome (GU112065) is 2,741 bp in length and genome is similar to that of monopartite begomoviruses originating from the Old World, with seven conserved ORFs. Further nucleotide (nts) sequence comparisons showed that the genome has the highest sequence identities of 96.1 %...

  6. Efeito de alguns herbicidas na cultura do quiabeiro, em testes efetuados em casa de vegetação Effect of some herbicides on okra crop in trials performed in the greenhouse

    Aldo Alves

    1970-01-01

    Full Text Available Duas variedades de quiabeiro foram testadas, em condições de casa de vegetação, com solo de textura areno-barrenta, na sua tolerância para 16 herbicidas, cada qual aplicado em uma única dose. Dentre os herbicidas aplicados em pré-plantio incorporado o triflu-ralin destacou-se no combate às ervas daninhas gramíneas e não causou nenhum dano para a planta do quiabeiro. Os herbicidas cloroxuron, linuron, norea e propachlor mostraram ótimos resultados no cambate às ervas más e quanto à tolerância dessa malvácea. Do grupo das triazinas, todos os herbicidas usados nas condições do teste provocaram danos para o quiabeiro.Two okra (Hibiscus esculentus L. varieties were tested for their tolerance to sixteen herbicides at one rate each, as a pre-planting and as a pre-emergence applications in greenhouse trials. The results with the incorporated herbicides showed that only tri-fluralin at 0,75 kg/ha active ingredient was promising to this crop. Different results were obtained with the pre-emergence applications of the group of urea and anilide herbicides. While no symptoms of injury were observed with chloroxuron, norea, linuron, and propachlor, injury symptoms were evident with diuron, fluometuron, metobromuron and siduron. The four triazine herbicides were very damaging to the okra plants. Under the conditions of these tests, only the herbicides trifluralin, chloroxuron, norea, and linuron were promising to the okra crop.

  7. Efeito de alguns herbicidas na cultura do quiabeiro, em testes efetuados em casa de vegetação / Effect of some herbicides on okra crop in trials performed in the greenhouse

    Aldo, Alves; Reinaldo, Forster.

    1970-01-01

    Full Text Available Duas variedades de quiabeiro foram testadas, em condições de casa de vegetação, com solo de textura areno-barrenta, na sua tolerância para 16 herbicidas, cada qual aplicado em uma única dose. Dentre os herbicidas aplicados em pré-plantio incorporado o triflu-ralin destacou-se no combate às ervas dan [...] inhas gramíneas e não causou nenhum dano para a planta do quiabeiro. Os herbicidas cloroxuron, linuron, norea e propachlor mostraram ótimos resultados no cambate às ervas más e quanto à tolerância dessa malvácea. Do grupo das triazinas, todos os herbicidas usados nas condições do teste provocaram danos para o quiabeiro. Abstract in english Two okra (Hibiscus esculentus L.) varieties were tested for their tolerance to sixteen herbicides at one rate each, as a pre-planting and as a pre-emergence applications in greenhouse trials. The results with the incorporated herbicides showed that only tri-fluralin at 0,75 kg/ha active ingredient w [...] as promising to this crop. Different results were obtained with the pre-emergence applications of the group of urea and anilide herbicides. While no symptoms of injury were observed with chloroxuron, norea, linuron, and propachlor, injury symptoms were evident with diuron, fluometuron, metobromuron and siduron. The four triazine herbicides were very damaging to the okra plants. Under the conditions of these tests, only the herbicides trifluralin, chloroxuron, norea, and linuron were promising to the okra crop.

  8. TOXICOLOGICAL TESTS WITH SEEDS FOR LEACHATE TREATMENT EVALUATION BY SLOW FILTRATION AND PHOTOCATALYSIS = ENSAIOS TOXICOLÓGICOS COM SEMENTES PARA AVALIAÇÃO DE TRATAMENTO DO CHORUME POR FILTRAÇÃO LENTA E FOTOCATÁLISE

    Núbia Natália Brito

    2010-01-01

    Full Text Available This work had as objective to study the techniques of Slow Filtration followed by heterogeneous photocatalysis (TiO2/UV in the leachate’ treatment originating from the sanitary landfill of Limeira-SP, City. Toxicological testes were used with seeds of Abelmoschus esculentus L.; Lactuca sativa L.; Impatiens balsamina and Celosia cristata to evaluate the treatment efficiency. The toxicological testes demonstrated the possibility of use larger concentration of leachate treated in the seeds germination, and it was possible to add 96% of leachate for the Abelmoschus esculentus L seeds germination, 30% for the Lactuca sativa L, 54% for Impatiens balsamina and 40% for Celosia cristata. Also were observed parameter values reductions of the environmental importance great, such as, coloration that presented reductions approximated 76,42%, total organic carbon (TOC 67,88%, total phenols 77,13% and amoniacal nitrogen 34,63%. The treatment methodology using Slow Filtration and Photocataysis demonstrated to be an excellent option of leachate remediation. = Este trabalho teve como objetivo estudar as técnicas de Filtração Lenta seguida de Fotocatálise heterogênea (TiO2/UV no tratamento de chorume proveniente do aterro sanitário da cidade de Limeira-SP. Foram empregados ensaios toxicológicos utilizando sementes de Abelmoschus esculentus L. (Quiabo; Lactuca sativa L. (Alface; Impatiens balsamina (Balsamina e Celosia cristata (Crista-de-galo, para avaliar a eficiência do tratamento. Os ensaios toxicológicos demonstraram a possibilidade de utilização de maior concentração do chorume tratado na germinação das sementes, sendo que foi possível adicionar 96% de chorume para a germinação das sementes de quiabo, 30% para a alface, 54% para a dobrada sortida e 40% para germinação das sementes de flores crista de galo. Também foram observadas reduções dos valores de parâmetros de grande importância ambiental, tais como, coloração que apresentou reduções em torno de 76,42%, Carbono Orgânico Total (COT 67,88%, fenóis totais 77,13% e nitrogênio amoniacal 34,63%. A metodologia de tratamento empregando Filtração Lenta e Fotocatálise demonstrou ser uma excelente opção de remediação de chorume.

  9. Efficacy of seed-based media for the mould-yeast conversion of Blastomyces dermatitidis.

    Chaturvedi, S; Randhawa, H S; Chaturvedi, V P; Khan, Z U

    1991-11-01

    The efficacy of 20 seed-based media is reported for the in vitro mould-yeast conversion of Blastomyces dermatitidis, employing pharmamedia agar, peptone glucose agar, glucose agar and water agar as controls. The mould-yeast conversion varied significantly according to the culture medium, fungal strains and incubation period (p less than 0.01). Garden-pea, chick-pea, cow-pea, soybean, peanut, green gram, French bean, lentil, okra and cottonseed converted all of the 7 B. dermatitidis test strains after 5 days of incubation at 37 degrees C. Although the efficacy of many of these seed media was found to be at par with pharmamedia agar - a commercial cottonseed embryo-derived protein, garden-pea seed agar is adopted because of the wider availability and low fat content of this seed. The recommended composition of the medium comprises 2% aqueous seed extract, 2% glucose and pH 6-7. Only nigerseed and sunflower seeds failed to support the conversion of B. dermatitidis. Of the control media, peptone glucose agar, glucose agar and water agar did not support the conversion of 2 of the B. dermatitidis test strains. The mechanism underlying variable mould-yeast conversion of B. dermatitidis on seed-based media is not clearly understood. However, most of the seeds supporting excellent mould-yeast conversion are known for their high protein content. The conversion was apparently not affected by the fat content of the seeds or by incorporation of glucose in the medium. PMID:1780002

  10. Project SEED.

    Chemical and Engineering News, 1986

    1986-01-01

    Reports on Project SEED (Summer Educational Experience for the Disadvantaged) a project in which high school students from low-income families work in summer jobs in a variety of academic, industrial, and government research labs. The program introduces the students to career possibilities in chemistry and to the advantages of higher education.…

  11. Diversidade genética em quiabeiro baseada em marcadores RAPD Genetic diversity in okra using RAPD markers

    Gilmar E. Martinello

    2003-03-01

    Full Text Available Avaliou-se a utilização de marcadores RAPD para estimar a diversidade em 42 acessos do gênero Abelmoschus e um de Hibiscus. As estimativas das distâncias genéticas foram feitas com base no complemento aritmético do Índice de Jaccard. Foram utilizadas as técnicas de análise multivariada, através de agrupamento hierárquico do vizinho mais próximo e método de Tocher, para estudar os arranjos dos grupos de genótipos, bem como analisar os métodos de agrupamentos empregados. Trinta e um iniciadores foram utilizados para amplificar fragmentos de DNA pela reação de polimerização em cadeia (PCR e foram gerados 103 fragmentos RAPD. O agrupamento hierárquico dos 43 genótipos com base no método do vizinho mais próximo separou os acessos, de modo geral, conforme as espécies botânicas, formando 6 grupos distintos. Isto foi confirmado pela projeção das distâncias genéticas no plano bidimensional, onde o primeiro e maior dos grupos reuniu os acessos de A. esculentus e A. caillei. Por outro lado, o método de Tocher reuniu 90% do germoplasma no grupo I incluindo, neste, os acessos de A. moschatus e A. manihot, além das outras duas espécies anteriores. O método de otimização de Tocher permitiu a formação de apenas 4 grupos de genótipos, mostrando-se coerente apenas em parte à análise de agrupamento hierárquico. Porém, o reagrupamento dos acessos do grupo I de Tocher pelo método hierárquico, revelou a existência de maior heterogeneidade genética no germoplasma estudado.RAPD markers were utilized to estimate the diversity among 42 Abelmoschus and 1 Hibiscus accessions . The genetic distances were based on the arithmetic complement of the Jaccard index. For this purpose we used the multivariate analysis technique by hierarchycal single linkage and the Tocher methods to obtain the genotypes agglomeration as well as to analyze the methods employed. Thirty-one random decamer primers were used do amplify DNA by the polymerase chain reaction (PCR and 103 RAPD fragments were generated. The hierarchycal method of single linkage has separated 43 genotypes, in a general way, according to the botanical species, forming six different groups. The genetic distances projection on the bidimentional level confirmed that the first and largest group has united A.esculentus and A. caillei accessions.On the other hand, the Tocher method grouped 90% of the germoplasm at group I, including A. moschatus and A. manihot accessions, besides the other two previous species. The Tocher optimization method allowed the formation of just 4 genotype groups. There was only partial coherence to the hierarchycal grouping analysis. The Tocher group I accessions regrouping by the hierarchycal method revealed the existence of a most important genetic heterogenity on the studied germplasm.

  12. Evaluation of wound healing effect of petroleum ether and methanolic extract of Abelmoschus manihot (L.) Medik., Malvaceae, and Wrightia tinctoria R. Br., Apocynaceae, in rats / Avaliação do efeito de cicatrização dos extratos de éter de petróleo e metanol de Abelmoschus manihot (L.) Medik., Malvaceae, e Wrightia tinctoria R. Br., Apocynaceae, em ratos

    Pritam S., Jain; Sanjay B., Bari.

    2010-11-01

    Full Text Available Nos últimos anos, o estresse oxidativo e radicais livres têm sido implicados na cicatrização. Abelmoschus manihot (L.) Medik., Malvaceae e Wrightia tinctoria R. Br., Apocynaceae, plantas utilizadas na medicina Ayurveda, possuem propriedades antiinflamatórias e antimicrobianas. O presente estudo foi [...] realizado para avaliar o potencial dos extratos de éter de petróleo e metanólico na cicatrização de feridas em ratos Wistar. Os ratos foram divididos em seis grupos com seis animais cada. O grupo 1 foi utilizado como controle, o grupo 2 recebeu a droga padrão e os outros quatro grupos foram tratados com duas doses diferentes de cada um dos extratos de A. manihot e W. tinctoria. Os parâmetros de cicatrização foram avaliados através da incisão feridas em ratos tratados com extrato, padrões e controles. Ambas as doses dos extratos de éter de petróleo e metanólico aumentaram significativamente força de ruptura da ferida quando comparados ao grupo controle. Abstract in english In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Abelmoschus manihot (L.) Medik., Malvaceae, and Wrightia tinctoria R. Br., Apocynaceae, plants widely used in Ayurveda, possesses anti-inflammatory and antimicrobial properties. The present study was [...] undertaken to assess the potential of petroleum ether and methanolic extracts in wound healing in Wistar albino rats. The rats were divided into six groups of six animals each. Group 1 is normal wounded control, group 2 received standard drug and the other four groups were treated with two different doses each of petroleum ether and methanolic extract of A. manihot and W. tinctoria. The wound healing parameters were evaluated by using incision wounds in extract-treated rats, standard and controls. Both the doses of petroleum ether and methanolic extract significantly increased wound breaking strength when compared with the control group.

  13. Seed Treatment. Bulletin 760.

    Lowery, Harvey C.

    This manual gives a definition of seed treatment, the types of seeds normally treated, diseases and insects commonly associated with seeds, fungicides and insecticides used, types of equipment used for seed treatment, and information on labeling and coloring of treated seed, pesticide carriers, binders, stickers, and safety precautions. (BB)

  14. Organic leek seed production - securing seed quality

    Deleuran, Lise Christina; Boelt, Birte

    seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield and...... germination percentage. Production in tunnels provided high yields of organic leek seed of good quality. Tunnel systems may be a set-up for organic seed production where small quantities of seed can be produced without compromising the high requirements of genetic purity and seed quality....... organic vegetable growers can get derogations to use non-organic seeds in their productions. Potentially, this could lead to the organic consumers’ loss of faith and interest in organic products. The pre-requisite for an organic vegetable production is the presence of organically produced high quality...

  15. Organic Leek Seed Production - Securing Seed Quality

    Deleuran, L C; Boelt, B

    2011-01-01

    seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield and...... germination percentage. Production in tunnels provided high yields of organic leek seed of good quality. Tunnel systems may be a set-up for organic seed production where small quantities of seed can be produced without compromising the high requirements of genetic purity and seed quality...... organic vegetable growers can get derogations to use non-organic seeds in their productions. Potentially, this could lead to the organic consumers’ loss of faith and interest in organic products. The pre-requisite for an organic vegetable production is the presence of organically produced high quality...

  16. Association of tomato leaf curl New Delhi virus DNA-B with bhendi yellow vein mosaic virus in okra showing yellow vein mosaic disease symptoms.

    Venkataravanappa, V; Lakshminarayana Reddy, C N; Jalali, S; Krishna Reddy, M

    2015-06-01

    Okra samples showing yellow vein mosaic, vein twisting and bushy appearance were collected from different locations of India during the surveys conducted between years 2005-2009. The dot blot and PCR detection revealed that 75.14% of the samples were associated with monopartite begomovirus and remaining samples with bipartite virus. Whitefly transmission was established for three samples representing widely separated geographical locations which are negative to betasatellites and associated with DNA-B. Genome components of these three representative isolates were cloned and sequenced. The analysis of DNA-A-like sequence revealed that three begomovirus isolates shared more than 93% nucleotide sequence identity with bhendi yellow vein mosaic virus from India (BYVMV), a monopartite begomovirus species that was reported previously as causative agent of bhendi yellow mosaic disease in association of bhendi yellow vein mosaic betasatellite. Further, the DNA-B-like sequences associated with the three virus isolates shared no more than 90% sequence identity with tomato leaf curl New Delhi virus (ToLCNDV). Analyses of putative iteron-binding sequence required for trans-replication suggests that begomovirus sequences shared compatible rep-binding iterons with DNA-B of ToLCNDV. Our data suggest that the monopartite begomovirus associated with okra yellow vein disease has captured DNA-B of ToLCNDV to infect okra. Widespread distribution of the complex shows the increasing trend of the capturing of DNA-B of ToLCNDV by monopartite begomoviruses in the Indian subcontinent. The recombination analysis showed that the DNA-A might have been derived from the inter-specific recombination of begomoviruses, while DNA-B was derived from the ToLCNDV infecting different hosts. PMID:26104329

  17. Comparative Evaluation of Zinc and Lead and their Synergistic Effects on Growth and Some Physiological Responses of Hassawi Okra (Hibiscus esculentus Seedlings

    M.A. Al-Omair

    2011-01-01

    Full Text Available Soil contamination with heavy metals has become a worldwide problem, leading to a reduction of plant growth and productivity. The objective of this study was carried out to compare the effect of different levels of zinc (Zn2+ or lead (Pb2+and their interactions on growth and physiological changes of Hassawi okra seedlings. These seedlings were grown in a soil, to which different levels (0, 5, 10, 20, 40, 80 mM of Zn2+ or Pb2+ were added with water (control, singly or in combinations. The results revealed that, okra seedlings growth were reduced gradually with increasing Zn2+ or/and Pb2+. These parameters were completely inhibited at 80 mM of Zn2+ or Pb2+, when they added in combination. While, 5 mM Zn2+ had a favorable effect on these growth parameters. The contents of chl. a and chl. b were significantly reduced as a result of exposure to Zn2+ or/and Pb2+. However, carotenoids content was increased at lower levels as compared with control. The increased of Zn2+ or/and Pb2+ levels inhibited sugars accumulation while the contents of protein, total free amino acids and proline were promoted. Pb2+ had more toxic effect than Zn2+. The toxicity caused by each metal alone was lower than that caused when they were added in combinations. Our data suggested that Zn2+ and Pb2+ exhibited synergistic effects on the growth and physiological responses of Hassawi okra seedlings, leading to injurious effects followed by death of these seedlings at 80 mM. Combined exposure to Zn2+ and Pb2+ enhanced or inhibited some of the effects, that were induced when only one metal was applied to growth medium.

  18. Comparative characterization of nucleotides, nucleosides and nucleobases in Abelmoschus manihot roots, stems, leaves and flowers during different growth periods by UPLC-TQ-MS/MS.

    Du, Le-yue; Qian, Da-wei; Jiang, Shu; Shang, Er-xin; Guo, Jian-ming; Liu, Pei; Su, Shu-lan; Duan, Jin-ao; Zhao, Min

    2015-12-01

    Nucleotides, nucleosides and nucleobases have been proven as important bioactive compounds related to many physiological processes. Abelmoschus manihot (L.) Medicus from the family of Malvaceae is an annual herbal plant of folk medicine widely distributed in Oceania and Asia. However, up to now, no detailed information could be available for the types and contents of nucleotides, nucleosides and nucleobases contained in A. manihot roots, stems, leaves as well as the flowers. In the present study, an UPLC-TQ-MS/MS method was established for detection of the twelve nucleotides, nucleosides and nucleobases. The validated method was successfully applied to identify the 12 analytes in different parts of A. manihot harvested at ten growth periods. 2'-deoxyinosine was not detected in all of the A. manihot samples. The data demonstrated that the distribution and concentration of the 12 compounds in A. manihot four parts were arranged in a decreasing order as leaf>flower>stem>root. Based on the results, the leaves and flowers of A. manihot could be developed as health products possessed nutraceutical and bioactive properties in the future. This method might also be utilized for the quality control of the A. manihot leaves and other herbal medicines being rich in nucleotides, nucleosides and nulecobases. PMID:26551204

  19. Comparative Effects of Abelmoschus esculentus (L Moench (Okro and Corchorus olitorius L (Jew Mallow on Soil Contaminated with Mixture of Petroleum Products

    Kelechi L. Njoku

    2012-04-01

    Full Text Available The comparative effects of Abelmoschus esculentus and Corchorus olitorius on soil contaminated with mixture of petroleum products were evaluated in this study. The pH, moisture, organic matter, microbial population and total petroleum hydrocarbon of the soils at the beginning and the end of the study were evaluated. Growth of A. esculentus led to loss of more TPH from the soil than the growth of C. olitorius. The growth of A. esculentus increased soil alkalinity and the soil moisture content more than that of C. olitorius. More bacteria and fungi were isolated from soil that had A. esculentus than from soil that had C. olitorius. Significant differences were noticed between the impacts of A. esculentus and that of C. olitorius on the soil contaminated with mixture of petroleum (p<0.05, p<0.01, p<0.001. The findings in this study show that A. esculentus has better ability to remediate petroleum contaminated soils than C. olitorius. Since both crops are easily propagated and readily grow in most soils, they can be very useful in combating the problems associated with contamination of soil with petroleum products.

  20. Períodos de interferência de plantas daninhas na cultura do quiabo Periods of weed interference in okra crop

    L.P.S Bachega

    2013-03-01

    Full Text Available Poucas pesquisas têm sido realizadas sobre interferência de plantas daninhas na cultura do quiabo. Objetivou-se com este trabalho estimar os períodos de interferência da comunidade infestante no quiabeiro. Um experimento de campo foi conduzido sob dois grupos de tratamentos, mantendo períodos crescentes de 0 (testemunha, 7, 21, 28, 35, 42, 49, 63, 77, 91 e 105 dias após a emergência da cultura (DAE, com e sem controle das plantas daninhas. As plantas daninhas com maior importância relativa foram Portulaca oleracea, Nicandra physaloides e Eleusine indica. A convivência do quiabeiro com as plantas daninhas por todo o ciclo de cultivo reduziu a produtividade da cultura em 95%. O período anterior à interferência foi de 57 DAE, enquanto o período total de prevenção à interferência foi de 14 DAE. Não houve período crítico de prevenção à interferência, sendo um único controle das plantas daninhas entre 14 e 57 DAE suficiente para prevenir a interferência na cultura do quiabo.Few research works have been carried out on weed interference in okra crop. The aim of this study was to estimate the periods of weed interference in okra crop. Thus, a field trial was carried out under two groups of treatments, using increased periods of 0 (check, 7, 21, 28, 35, 42, 49, 63, 77, 91, and 105 days after crop emergence (DAE with weed control and without weed control. Weeds with highest relative importance were Portulaca oleracea, Nicandra physaloides, and Eleusine indica. Coexistence of okra crop and weeds throughout the season reduced crop yield by 95%. The period before interference was 57 DAE, while total period of interference prevention was 14 DAE. There was no critical period of interference prevention, with a single weed control between 14 and 57 DAE being sufficient for interference prevention in okra crop.

  1. Efeito da temperatura e reação de genótipos de quiabeiro ao mofo branco / Effect of temperature and reaction of okra genotypes to white mold

    Ivan Herman, Fischer; Mirian de Souza, Filetti; Juliana Cristina Sodário, Cruz; César Júnior, Bueno.

    2014-03-01

    Full Text Available O quiabeiro é suscetível a várias doenças que podem causar prejuízos ao agricultor, caso não sejam manejadas. Objetivaram-se avaliar o efeito da temperatura e a reação de genótipos de quiabeiro ao mofo branco. Plantas de 30 dias inoculadas na região do colo, com e sem ferimento, com dois isolados de [...] Sclerotinia sclerotiorum, foram mantidas por duas semanas nas temperaturas de 15; 20; 25; 30 ou 35ºC. Quatorze genótipos de quiabeiro com um e dois meses de emergência, assim como frutos no ponto de colheita comercial tiveram seus comportamentos avaliados quanto à doença. Avaliaram-se a incidência de plantas com tombamento ou mortas e o comprimento da lesão nos frutos inoculados. A doença foi favorecida por temperaturas entre 15 e 20ºC e, de maneira geral, não foram observadas diferenças entre os isolados e influência do ferimento na ocorrência da doença. Não foram encontrados genótipos de quiabeiro resistentes ao mofo branco, pois todos apresentaram incidência superior a 30% de plantas mortas, sendo as plantas mais novas mais suscetíveis, e comprimento de lesão nos frutos >6,0 cm. Entretanto, diferenças de suscetibilidade entre os genótipos foram observadas em plantas de 30 e de 60 dias e podem ser informações úteis aos produtores para escolha de cultivares em clima ameno, assim como ser exploradas em programas de melhoramento. Abstract in english The okra plant is susceptible to several diseases that can cause losses to the farmer if not managed. This study aimed to evaluate the effect of temperature and the reaction of okra genotypes to white mold. Plants aged 30 days, inoculated in the collar region, injured or not, including two strains o [...] f Sclerotinia sclerotiorum, were maintained for two weeks at temperatures of 15, 20, 25, 30 or 35ºC. Fourteen okra genotypes at one and two months after emergence, as well as fruits at commercial harvest time, had their behavior evaluated for the disease. Damping-off or dead plants were evaluated, as well as the injure length in the inoculated fruits. The disease was favored by temperatures between 15 and 20ºC and, in general, no differences were observed between the isolates and the influence of the injury on the disease occurrence. There were no okra genotypes resistant to white mold, since all of them had more than 30% of incidence of dead plants; younger plants were more susceptible and the injure length in fruits was > 6.0 cm. However, differences in the susceptibility between genotypes were observed for plants aged 30 and 60 days, which could constitute useful information for producers in the choice of cultivars under moderate climate and could be explored in breeding programs.

  2. Oil palm seed distribution

    Durand-Gasselin Tristan; Cochard Benoît

    2005-01-01

    For a tropical plant, the oil palm commodity chain has the peculiarity of possessing a major seed production sector for reasons that are primarily genetic. This seed sector has numerous original aspects. Breeders are also propagators and usually also distribute their seeds. Oil palm seeds are semi-recalcitrant: they display pseudo-dormancy. Achieving seed germination is difficult and requires lengthy treatments and special installations. This restriction greatly influences seed distrib...

  3. Isolating, screening and applying chromium reducing bacteria to promote growth and yield of okra (Hibiscus esculentus L.) in chromium contaminated soils.

    Maqbool, Zahid; Asghar, Hafiz Naeem; Shahzad, Tanvir; Hussain, Sabir; Riaz, Muhammad; Ali, Shafaqat; Arif, Muhammad Saleem; Maqsood, Marium

    2015-04-01

    Hexavalent chromium [Cr (VI)], extensively used in different industries, is one of the most toxic heavy metals. The Cr (VI) reducing bacteria could be helpful in decreasing its toxic effects. The present study was conducted to evaluate the potential of Cr (VI) reducing bacteria to improve growth and yield of okra (Hibiscus esculentus L.) in Cr-contaminated soils. Most of the selected bacterial isolates significantly increased the growth and yield of okra. Maximum response was observed in the plants inoculated with the isolate K12 where plant height, root length, fruit weight and number of fruits per plant increased up to 77.5 percent, 72.6 percent, 1.4 fold and 2.9 fold, respectively. Moreover, inoculation with bacteria caused significant decrease in Cr (VI) concentration in soil and plant parts across all treatments. The maximum decrease of 69.6, 56.1 and 40.0 percent in Cr (VI) concentrations in soil, plant vegetative parts and plant reproductive parts, respectively, was observed in the treatment inoculated with the strain K12. Based on amplification, sequencing and analysis of 16S rDNA sequence, the strain K12 was found belonging to genus Brucella and was designated as Brucella sp. K12. These findings suggest that the strain K12 may serve as a potential bioresource to improve crop production in Cr-contaminated soils. PMID:25066609

  4. Seed health and vigor

    The health of lentil and chickpea seed greatly impacts the quality of the crop stand and yield. Healthy seed has a high germination rate, is whole (free of cracks or other damage), is free from foreign matter including weed seed and has limited seedborne pathogens. The health of the seed often dep...

  5. Effects of simulated oilfield produced water on early seedling growth after treatment in a pilot-scale constructed wetland system.

    Pardue, Michael J; Castle, James W; Rodgers, John H; Huddleston, George M

    2015-01-01

    Seed germination and early seedling growth bioassays were used to evaluate phytotoxicity of simulated oilfield produced water (OPW) before and after treatment in a subsurface-flow, pilot-scale constructed wetland treatment system (CWTS). Responses to untreated and treated OPW were compared among seven plant species, including three monocotyledons: corn (Zea mays), millet (Panicum miliaceum), and sorghum (Sorghum bicolor); and four dicotyledons: lettuce (Lactuca sativa), okra (Abelmoschus esculents), watermelon (Citrullus lanatus), and soybean (Glycine max). Phytotoxicity was greater in untreated OPW than in treated OPW. Exposures to untreated and treated OPW enhanced growth in some plant species (sorghum, millet, okra, and corn) relative to a negative control and reduced growth in other plant species (lettuce, soybean, and watermelon). Early seedling growth parameters indicated that dicotyledons were more sensitive to test waters compared to monocotyledons, suggesting that morphological differences between plant species affected phytotoxicity. Results indicated the following sensitivity scale for plant species: lettuce>soybean>watermelon>corn>okra?millet>sorghum. Phytotoxicity of the treated OPW to lettuce and soybean, although concentrations of COCs were less than irrigation guideline concentrations, suggests that chemical characterization and comparison to guideline concentrations alone may not be sufficient to evaluate water for use in growing crops. PMID:25409245

  6. Temperature and modified atmosphere affect the quality of okra Temperatura e atmosfera modificada influenciam a qualidade do quiabo

    Fernando Luiz Finger

    2008-01-01

    Full Text Available Little information is available on the influence of temperature on plastic films wrapped okra (Albelmoschus esculentus for their postharvested conservation. This works investigated the influence of the temperature and PVC film on the development of chilling injury and storability from one of the most popular Brazilian cultivar of okra cv. Amarelinho in fruits stored at 5, 10ºC and at 25ºC. Fruits were harvest at commercial maturity stage with length ranging from 8 to 12 cm, and immediately wrapped in PVC over a polystyrene tray and than stored until visible deterioration or wilting symptoms. Lowering the temperature of storage room from 25 to 10 or 5ºC decreased the weight loss in both PVC wrapped and control fruits, with a lower rate at 5ºC. By reducing the temperature to 5 or 10ºC and wrapping the fruits in PVC film, the relative water content of the fruit pericarp was maintained throughout the storage, while at 25ºC the high weight loss was associated with significant reduction of the water content. The development of chilling symptoms was delayed by the presence of PVC film in fruits stored at 5ºC. However, at 10ºC symptoms of pitting were not developed in PVC wrapped or control fruits up to tenth day of storage. The rate of chlorophyll degradation was diminished by reducing the temperature and by wrapping the fruits with PVC film. The appearance of severe chilling symptoms at 5ºC was associated to less chlorophyll in the fruit pericarp on the control as compared to their content in the PVC wrapped fruits.Para o armazenamento do quiabo (Albelmoschus esculentus há poucas informações disponíveis sobre a influência da temperatura e filmes plásticos na conservação pós-colheita desta hortaliça. Para investigar a influência da temperatura e do filme de PVC na qualidade e desenvolvimento de sintomas de injúria por frio de uma das mais populares cultivares brasileira de quiabo cv. Amarelinho, os frutos foram armazenados a 5, 10 e 25ºC. Os frutos foram colhidos no estádio de maturação comercial, com comprimento entre 8 e 12 cm, sendo então envoltos por uma camada de filme de PVC em bandeja de poliestireno expandido, seguido do armazenamento até o aparecimento de sintomas de deterioração ou murcha. A redução da temperatura de armazenamento de 25 para 10 ou 5ºC diminuiu a perda de massa nos frutos envoltos com PVC e dos frutos controle, com menor taxa a 5ºC. Ao reduzir a temperatura para 5 ou 10ºC e cobrir com filme de PVC, o conteúdo relativo de água foi mantido durante o armazenamento, porém a 25ºC a elevada perda de massa esteve associada a significante redução do conteúdo de água do pericarpo do fruto. O aparecimento de sintomas de injúria por frio foi retardado pela presença de filme de PVC nos frutos armazenados a 5ºC. No entanto a 10ºC, não foram detectados sintomas de degeneração associados à injúria por frio nos frutos envoltos com PVC ou controle até o décimo dia de armazenamento. A taxa de degradação de clorofila durante o armazenamento foi menor nos frutos mantidos nas temperaturas mais baixas e com filme PVC. O aparecimento de sintomas severos de injúria por frio a 5ºC relacionou-se com o menor conteúdo de clorofila dos frutos controle, quando comparado àqueles envoltos em filme de PVC.

  7. Growth, Yield and WUE of Drip and Sprinkler Irrigated Okra Grown On Sandy Soil Under Semi-Arid Conditions in Southeast Ghana

    Plauborg, Finn

    weekly fertigation (8 times from two weeks after emergence) was done during the second experiment. The treatment effects on growth, yield, biomass, water use efficiency of the economical fresh pod yield (WUEy), and on the total above ground biomass (WUEtbm) were studied. The results showed that the okra......Vegetable production systems at the Keta sand spit, Southeast Ghana, are typically managed with excessive amounts of irrigation water and fertilizers on sandy soils with low inherent water and nutrient retention capacities. The shallow groundwater which is the primary irrigation water resource is...... prone to salinization from the Keta lagoon, the Atlantic Ocean and brackish water underneath (Kortatsi and Agyeku, 1999). To ensure the sustainability of vegetable production at the Keta spit, introduction of water saving irrigation systems and improved irrigation management schemes are important. Thus...

  8. Grape Seed Extract

    ... R S T U V W X Y Z Grape Seed Extract Share: On This Page Introduction What ... Effects and Cautions For More Information Key References grape_seed_extract.jpg © Steven Foster Common Name: grape ...

  9. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds (crop..., or type may be expressed as “crop seeds” or “other crop seeds,” but the percentage shall...

  10. Modeling the Time Elapsing from Seed Sowing to Emergence in Some Vegetable Crops

    Sezgin Uzun

    2001-01-01

    Full Text Available A simple regression model based on mean temperature was developed to be used for predicting the time elapsing from seed sowing to seedling emergence for some vegetable crops, namely tomato (Lycopersicon esculentum, Mill., pepper (Capsicum annum, L., aubergine (Solanum melongena, L., pea (Pisum sativum, L., carrot (Daucus carota, L., sweat corn (Zea mays, cabbage (Brassica oleraceae L.var. capitata (L Alef, cauliflower Brassica oleraceae L.var. botrytis L, onion (Allium cepa, L., celery (Apium graviolens, L., lettuce (Lactuca sativa, L., parsley (Petroselinum hortense , garden beet (Beta vulgaris, L., cucumber (Cucumis sativus, L., melon (Cucumis melo, L., runner bean (Phaseolus vulgaris, L., watermelon (Citrullus lanatus, Thunb., okra (Hibiscus esculentus, L., asparagus (Asparagus officinalis, L., spinach (Spinacia oleracea, L., radish (Rhaphanus sativus, L. and turnip (Brassica rapa, L.. The prediction performance of the model with respect to the data used was highly acceptable. R2 values of regression co-efficients for each crop varied from 0.94 to 0.99 depending on the species. Plotting the actual days from seed sowing to emergence for all the crops against the predicted ones showed that the prediction performance of the model was good explaining 98% of the variation for combined data from all the crops. The present model also predicted optimum temperatures (To for tried vegetables in the limits of acceptability.

  11. “Seed Proteomics"

    Proteomic analysis of seeds encounters some specific problems that do not impinge on analyses of other plant cells, tissues, or organs. There are anatomic considerations. Seeds comprise the seed coat, the storage organ(s), and the embryonic axis. Are these to be studied individually or as a compo...

  12. Growth and accumulation of nutrients of the okra cropsCrescimento e acúmulo de nutrientes da cultura do quiabeiro

    Anarlete Ursulino Alves

    2013-03-01

    Full Text Available With the objective to quantify the okra crop’s growth and macro and micronutrients accumulation during its life-cycle obtaining equations that best represent it, an experiment was carried out at Jaboticabal city, SP, Brazil, from January 14th to May 14th, 2009. The cv. Santa Cruz 47 was directly sowed into open soil furrows, in a spacing of 1 x 0,2 m. During the life-cycle were realized random samples of plants at 15 days intervals. The means were adjusted to polynomial regression equations set of non-linear parameters. After 50 days from sowing there has been a large increase of dry matter in plant’ parts. At the final harvest, 120 days after sowing, the leaves, stems and fruits accounted for 21, 71 and 8%, respectively, from the total plant dry matter. The decreasing sequence of nutrient accumulation was K, Ca, N, Mg, P, Fe, B, Mn, Zn and Cu equivalent to 6,002.8; 4,733.8; 2,930.8; 1,196.3; 473.7; 436.2; 49.8; 10.4; 7.1; 7.1 e 1.5 mg plant-1. The quantity accumulated of culture were 146.5; 23.7; 300.1; 236.7; 59.8 e 21.8 kg ha-1 of N, P, K, Ca, Mg and S, respectively, and 520.0; 76.0; 2,491.0; 355.0 e 355.0 de B, Cu, Fe, Mn and Zn, respectively. Com o objetivo de quantificar o crescimento e o acúmulo de macro e micronutrientes da cultura do quiabeiro, no decorrer do ciclo, e obter equações que melhor os representem, um experimento foi realizado de 14 de janeiro a 14 de maio de 2009, na UNESP Câmpus de Jaboticabal, SP. A cv. Santa Cruz 47 foi semeada diretamente no sulco, em espaçamento de 1 x 0,2 m. No decorrer do ciclo, realizaramse coletas aleatórias de plantas com intervalos de quinze dias. As médias foram ajustadas às equações de regressão polinomial e de modelos não lineares. A partir de 50 dias após a semeadura (DAS houve grande aumento no acúmulo de matéria seca nas partes da planta. Na colheita final, 120 DAS, as folhas, hastes e frutos representaram 21, 71 e 8%, respectivamente, da matéria seca total da planta. A seqüência decrescente de acúmulo de nutrientes pelo quiabeiro foi K, Ca, N, Mg, P, S, Fe, B, Mn, Zn e Cu, o equivalente a 6.002,8; 4.733,8; 2.930,8; 1.196,3; 473,7; 436,2; 49,82; 10,4; 7,1; 7,1 e 1,52 mg planta-1. As quantidades acumuladas pela cultura foram 146,5; 23,7; 300,1; 236,7; 59,8 e 21,8 kg ha-1 de N, P, K, Ca, Mg e S, respectivamente, e de 520,0; 76,0; 2.491,0; 355,0 e 355,0 g ha-1 de B, Cu, Fe, Mn e Zn, respectivamente.

  13. Radon seed implants

    Radon seeds, formerly used for vascular and neoplastic tumors, acne, and other dermatological disorders, are rarely, if ever, used today. Because the half-life of radon is 3.83 days, these hollow gold seeds filled with radon gas are usually left in situ permanently. A case is reported of a woman who had seeds implanted 33 years ago for a vascular lesion. The seeds were removed and found to have minute amounts of residual radiation but not sufficient to cause radiation damage. Since seeds are foreign bodies, removal is recommended if they are easily accessible. (U.S.)

  14. Oil palm seed distribution

    Durand-Gasselin Tristan

    2005-03-01

    Full Text Available For a tropical plant, the oil palm commodity chain has the peculiarity of possessing a major seed production sector for reasons that are primarily genetic. This seed sector has numerous original aspects. Breeders are also propagators and usually also distribute their seeds. Oil palm seeds are semi-recalcitrant: they display pseudo-dormancy. Achieving seed germination is difficult and requires lengthy treatments and special installations. This restriction greatly influences seed distribution and the role of the different stakeholders in the commodity chain. It was only once it had been discovered how the “sh” gene functioned, which controls shell thickness, and when it became necessary to produce “tenera” seeds derived from exclusively “dura x pisifera” crosses, that a true seed market developed. In addition it is difficult to organize seed distribution to smallholders. This is partly due to difficulties that the profession, or a State-run organization, has in controlling middlemen networks, and partly to the absence of any protective systems (UPOV, plant breeder certificate, etc. that generally oblige breeders to preserve and propagate parents in their own installations. In fact there are major inequalities in the access to seeds between agroindustry and smallholders. Another peculiarity of the oil palm seed market is the virtually total absence of guarantees for buyers: the quality of the research conducted by breeders, the seed production strategies necessary for transferring genetic progress, and the technical quality of production. The only guarantee today comes from the relations of confidence established year after year between breeders/distributors and growers. In this fields, research can lead to some proposals: molecular biology offers some interesting prospects for certifying seed quality and social science develop effective communication methods.

  15. MATURATION OF POPCORN SEEDS

    ANDRÉIA MÁRCIA SANTOS DE SOUZA DAVID

    2003-12-01

    Full Text Available The objective of this study was to analyze the maturation process of the popcorn seeds DFT 2 (selection cycle 2. At approximately 30 days after full blooming, when about 50% of the plants exhibited receptive silks, the first harvest of the seeds was accomplished. The other harvests were accomplished at 7-day intervals until seeds reached approximately 12% moisture content (wet basis, which occurred upon the 10th harvesting. Just after each harvest, the seeds were manually husked, to determine their moisture content and the weight of the dry matter. Also the development of the black layer was visually evaluated. After the last harvesting, the seeds were evaluated for retention on sieves, germination and vigor (modified cold, accelerated aging and electric conductivity tests. The popcorn seeds reach the maximum dry matter weight (mass maturity 68 days after flowering, and the physiological maturity of the seeds (maximum germination and vigor achieves it from 62 to 68 days after blooming. The seeds show humidity in the grade from 17 to 20%, for occasion of the physiological maturity and mass maturity. The black layer is an efficient visual characteristic to identify the physiological maturity of the seeds.

  16. What Are Chia Seeds?

    ... Potassium Foods 4 Types of Foods to Help Boost Your Memory 4 Types of Foods to Help Boost Your Memory tea and herbs - 4 Metabolism Myths ... is made by soaking chia seeds in fruit juice or water. Chia seeds are very absorbent and ...

  17. Neutron irradiation of seeds

    Neutrons are a valuable type of ionizing radiation for seed irradiation and radiobiological studies and for inducing mutations in crop plants. In experiments where neutrons are used in research reactors for seed irradiation it is difficult to measure the dose accurately and therefore to establish significant comparisons between experimental results obtained in various reactors and between repeated experiments in the same reactor. A further obstacle lies in the nature and response of the seeds themselves and the variety of ways in which they are exposed in reactors. The International Atomic Energy Agency decided to initiate international efforts to improve and standardize methods of exposing seeds in research reactors and of measuring and reporting the neutron dose. For this purpose, an International Neutron Seed Irradiation Programme has been established. The present report aims to give a brief but comprehensive picture of the work so far done in this programme. Refs, figs and tabs

  18. Alginate-okra gum blend beads of diclofenac sodium from aqueous template using ZnSO4 as a cross-linker.

    Sinha, Priyanka; Ubaidulla, U; Hasnain, M Saquib; Nayak, Amit Kumar; Rama, Bobba

    2015-08-01

    Zinc (Zn(2+))-ion induced diclofenac sodium (DS)-loaded alginate-okra (Hibiscus esculentus) gum (OG) blend beads was successfully formulated through Zn(2+)-ion induced ionic-gelation cross-linking method in a complete aqueous environment. Effects of polymer-blend ratio and cross-linker concentration on drug encapsulation efficiency (DEE) and cumulative drug release at 8 h (R8h) were optimized by 3(2)-factorial design. The optimized formulation of Zn(2+)-ion induced DS-loaded alginate-OG beads demonstrated 89.27±3.58% of DEE and 43.73±2.83% of R8h. The bead sizes were within 1.10±0.07 to 1.38±0.14 mm. The bead surface morphology was analyzed by SEM. The drug-polymer interaction in the optimized bead matrix was analyzed by FTIR and P-XRD. These beads exhibited sustained in vitro drug release over a prolonged period of 8h and followed controlled-release (zero-order) pattern with super case-II transport mechanism. The swelling and degradation of the optimized beads was influenced by the pH of test mediums, which might be suitable for intestinal drug delivery. PMID:25987461

  19. A comparative study of Okra gum on controlled release kinetics and other formulation characteristics of Tramadol HCl extended release matrix tablets Vs Synthetic hydrophilic polymers.

    Amaldoss Maria john Newton

    2015-03-01

    Full Text Available The main aim of this investigation was to develop a sustained release matrix tablets of Tramadol HCl using hydrophilic polymers such as hydroxyl propyl methyl cellulose (HPMC, in different grades (E15LV, K4M, K100M and compare the various parameters against the natural polysaccharide Okra gum. The polymer proportions are used in different concentration in order to optimize the correct proportion of polymer to achieve controlled release profile. The matrix tablets were prepared by direct compression technique which is more industrially relevant. A small quantity of Carbopol was also incorporated in the formulation to give bio-adhesiveness & improved compression characteristics. The formulations were studied for pre-compression parameters and post-compression parameters. The in vitro drug release study was performed in 0.1N HCl (pH 1.2 for 1.5 hour and phosphate buffer (pH 6.8 were upto 12 hours. The study results revealed that the matrix tablet can be developed with the used polymers without any tablet manufacturing defects in optimized polymer concentration.. The polymers could control the drug release in various levels according to the concentration present in the formulation. The drug release profile was fitted with various pharmacokinetics models. The formulations showed the different degree of fit with different kinetic models. The drug release mechanism involves the combined process of diffusion, swelling and erosion.

  20. Seed recovery and regeneration

    Magnetohydrodynamic's (MHD) electric power generation capability depends on Faraday's law of induction. An ionized gas, called plasma, passes through a fixed magnetic field. The plasma is produced by seeding flue gases at a temperature of around 2500 deg C with potassium carbonate or formate. In a coal-fired open cycle MHD power plant the potassium seed material is in direct contact with the polluted flue gas from coal combustion. The molten slag droplets will capture a certain amount of potassium. The sulphur dioxide in the flue gases will react with potassium to potassium sulphate. The spent seed material has to be recovered and reprocessed so that the potassium can be reused as seed material. We can distinguish a preprocessing and a regeneration step. Seed preprocessing removes the minerals and volatile coal constituents from the potassium salts. Seed regeneration transforms the purified potassium sulphate into potassium carbonate or formate. Eight regeneration processes have been evaluated. The processes that convert the sulphur of the coal into saleable sulphur or sulphuric acid are still at an early stage of development. Considering all pros and cons, the Formate/Econoseed process appears most suited for seed regeneration purposes in coal-fired MHD systems. For an advanced MHD power plant the mass flow rates of the flue gas and of the potassium containing off-product streams will be calculated. It will be shown that the sulphur content of coal will influence the economical efficiency of the power plant

  1. Glioblastoma with spinal seeding

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  2. Glioblastoma with spinal seeding

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C. [Clinical Div. of Oncology, Dept. of Medicine I, Univ. of Vienna (Austria); Czech, T. [Dept. of Neurosurgery, Univ. of Vienna (Austria); Diekmann, K. [Dept. of Radiooncology, Univ. of Vienna (Austria); Birner, P.; Hainfellner, J.A. [Clinical Inst. for Neurology, Univ. of Vienna (Austria); Prayer, D. [Dept. of Neuroradiology, Univ. of Vienna (Austria)

    2004-07-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  3. Prescribed seed plantings

    US Fish and Wildlife Service, Department of the Interior — This document contains memos, notes, and tables related to tallgrass prairie seed harvesting on Neal Smith National Wildlife Refuge in 1995.

  4. Oil seed marketing prospects

    With its 100 million tonnes annual production, the American continent is by far the world's biggest producer of oil seed, followed by Asia - 52 million, and Europe - 27 million tonnes. The Italian and European Communities have the farming capacity to double their production, but international agreements currently prohibit such initiatives. After first providing a panorama of the world oil seed market, this paper discusses new reforms in European Communities internal agricultural policies which currently limit production. These reforms, intended to encourage the production of oil seed for use as an ecological automotive fuel alternative, call for an obligatory set-aside of 15% of producing farm-land in exchange for the compensatory removal of oil seed production limits

  5. Tomato seeds for LDEF

    1983-01-01

    Tomato seeds are prepared for their launch aboard the Langley's Long Duration Exposure Facility. Photograph published in Winds of Change, 75th Anniversary NASA publication (page 119), by James Schultz.

  6. Seed systems support in Kenya

    Munyi, Peter; Jonge, de, B.

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector Development (ISSD) approach. The ISSD approach has evolved as a response to the almost exclusive focus on formal seed systems in seed sector development programs. Instead, ISSD aims to recognize and sup...

  7. Preface

    Kenaf (Hibiscus cannabinus L., Malvaceae) is a warm season annual fiber crop closely related to cotton (Gossypium hirsutum L., Malvaceae) and okra (Abelmoschus esculentus L. Moench, Malvaceae) that can be successfully produced in a large portion of the United States, particularly in the southern sta...

  8. UTILIZATION OF VERMIWASH POTENTIAL ON CERTAIN SUMMER VEGETABLE CROPS

    GORAKH NATH

    2010-06-01

    Full Text Available The study was carried out to evaluate the impact of vermiwash on the growth, flowering and productivity of okra (Abelmoschus esculantus, lobia (Vigna unguiculata and radish (Raphnus sativus. Vermiwash was extracted from different vermicompost which was composted from different animal agro and kitchen wastes through earthworm Eisenia foetida. It was observed that treatment of vermiwash shows significantly increased in growth and productivity and decreased flowering period. The maximum significant growth was observed in 30 mg/m2 concentration of combination of buffalo dung with rice bran 38.0±1.3 cm. in case of okra, 30 mg/m2 concentration of combination of buffalo dung with gram bran (seed of Cicer arientinum 215.5±5.2 cm., in lobia crops and 30 mg/m2 concentration of buffalo dung with gram bran 20.4±1.4 cm. The significant early starting of flowering and increased in productivity was found in all treated groups with respect to control.

  9. Muskmelon seed priming in relation to seed vigor

    Nascimento Warley Marcos

    2004-01-01

    Full Text Available A number of important factors may affect seed priming response, including seed quality. Effects of seed vigor on seed priming response were investigated using seed lots of two muskmelon (Cucumis melo L. cultivars. Seeds of muskmelon, cvs. Mission and Top Net SR were artificially aged at 43°C for 0, 20 and 40 hours. Seeds were primed for six days in darkness at 25°C in KNO3 (0.35 mol L-1 aerated solution. Aged seeds germinated poorly at 17°C. Priming increased germination rate at 17 and 25°C and germination percentage at 17°C. An interaction effect on germination performance between vigor and priming was observed, especially at low temperature. Priming increased germination performance in seeds of low vigor, and the response was cultivar dependent.

  10. Composição mineral de frutos de quatro cultivares de quiabeiro Mineral composition of four cultivars of okra fruits

    Wagner Ferreira da Mota

    2008-06-01

    Full Text Available Este experimento foi realizado na Universidade Federal de Viçosa e teve como objetivo avaliar a composição mineral dos frutos de quatro cultivares de quiabo. O delineamento utilizado foi em blocos casualizados, sendo os tratamentos constituídos pelas cultivares Amarelinho, Red Velvet, Star of David e Mammoth Spinless, com cinco blocos. Os maiores teores de cálcio, fósforo, enxofre e magnésio foram encontrado na Cultivar Red Velvet. Os maiores teores de nitrogênio total, amônio e manganês e menor teor de fósforo foram encontrados na cultivar Mammoth Spinless, enquanto nas cultivares Red Velvet e Star of David foram observados os menores teores de nitrogênio total, amônio e manganês. Menores teores de cálcio, enxofre e magnésio foram verificados em conjunto nas cultivares Amarelinho, Star of David e Mammoth Spinless.This experiment was carried out at Universidade Federal de Viçosa and had the objective to evaluate the mineral composition of four cultivars of okra fruits. The experimental design was in randomized blocks, where the treatments were composed by the cultivars Amarelinho, Red Velvet, Star of David and Mammoth Spinless, with five blocks. The cultivar Red Velvet had the highest content of calcium, phosphorus, sulfur and magnesium. The cultivar Mammoth Spinless had the highest content of total nitrogen, ammonium and manganese and the lowest content of phosphorus. The cultivars Red Velvet and Star of David had the lowest contents of total nitrogen, ammonium, and manganese. Lower contents of calcium, sulfur and magnesium were present in the cultivars Amarelinho, Star of David and Mammoth Spinless.

  11. Seed-borne pathogens and electrical conductivity of soybean seeds

    Adriana Luiza Wain-Tassi

    2012-02-01

    Full Text Available Adequate procedures to evaluate seed vigor are important. Regarding the electrical conductivity test (EC, the interference in the test results caused by seed-borne pathogens has not been clarified. This research was carried out to study the influence of Phomopsis sojae (Leh. and Colletotrichum dematium (Pers. ex Fr. Grove var. truncata (Schw. Arx. fungi on EC results. Soybean seeds (Glycine max L. were inoculated with those fungi using potato, agar and dextrose (PDA medium with manitol (-1.0 MPa and incubated for 20 h at 25 °C. The colony diameter, index of mycelial growth, seed water content, occurrence of seed-borne pathogens, physiological potential of the seeds, measured by germination and vigor tests (seed germination index, cold test, accelerated aging and electrical conductivity, and seedling field emergence were determined. The contents of K+, Ca2+, and Mg2+ in the seed and in the soaking solution were also determined. A complete 2 × 4 factorial design with two seed sizes (5.5 and 6.5 mm and four treatments (control, seeds incubated without fungi, seeds incubated with Phomopsis and seeds incubated with Colletotrichum were used with eight (5.5 mm large seeds and six (6.5 mm large seeds replications. All seeds submitted to PDA medium had their germination reduced in comparison to the control seeds. This reduction was also observed when seed vigor and leached ions were considered. The presence of Phomopsis sojae fungus in soybean seed samples submitted to the EC test may be the cause of misleading results.

  12. Nematofauna associada à cultura do quiabo na região leste de Minas Gerais The influence of parasitic nematodes on okra crop in eastern Minas Gerais State, Brazil

    Rosângela D'Arc de Lima Oliveira

    2007-03-01

    Full Text Available A cultura do quiabo possui importância sócio-cultural para o estado de Minas Gerais (MG e 34,2% do volume de quiabo comercializado na Ceasa/Contagem procede dos municípios localizados entre Caratinga e Governador Valadares. Entretanto, o quiabeiro tem a sua produção influenciada pelos danos decorrentes de infecções causadas pelos nematóides das galhas (Meloidogyne spp.. As principais espécies desse nematóide que atacam o quiabeiro já foram relatadas no Brasil, e algumas destas podem causar a morte da planta. A correta identificação da(s espécie(s e, ou da(s raça(s de Meloidogyne presente(s nas raízes do quiabeiro é importante na escolha da medida de controle mais apropriada. Para determinar a ocorrência e distribuição de Meloidogyne spp. e outros nematóides na região leste de MG, 70 amostras de solo e raízes da cultura, provenientes de 14 localidades, foram avaliadas por características morfológicas e isoenzimáticas. Dentre as populações de Meloidogyne spp. identificadas prevaleceu M. incognita (fenótipos de Esterase I1 e I2, seguida de M. javanica (fenótipos J2 e J3 e M. arenaria (fenótipo A2. A espécie M. mayaguensis foi confirmada pela ocorrência do fenótipo M2 para esterase, N3 para malato desidrogenase, N2 para superóxido dismutase e N3 para glutamato-oxaloacetato transaminase. Este é o primeiro relato da ocorrência de M. mayaguensis em MG. Outros nematóides detectados na rizosfera do quiabeiro foram Aphelenchus sp., Criconemella sp., Helicotylenchus spp., Pratylenchus brachyurus e P. coffeae, Rotylenchulus reniformis, Rotylenchus sp., Tylenchus sp. e Tylenchorhynchus sp.Okra has a cultural and social importance for the State of Minas Gerais (MG, and 34,2% of the volume marketed in the Ceasa/Contagem, MG, come from the municipalities located near Caratinga and Governador Valadares. The okra production is influenced for the infection caused by the root-knot nematodes (Meloidogyne spp.. The main species of these nematodes attacking okra have already been reported in Brazil, and some of them can cause plant death. The correct identification of Meloidogyne species and, or races infecting okra roots is mandatory for choosing appropriate control measures. To determine the occurrence and distribution of Meloidogyne spp. and other nematodes in the area of the East of MG, 70 soil and root samples coming from 14 municipalities were appraised for morphological and isoenzymatic characteristics. Among the populations of Meloidogyne spp., M. incognita (Esterase phenotypes I1 and I2 prevailed and were followed by M. javanica (phenotypes J2 and J3 and M. arenaria (phenotypes A2. The species M. mayaguensis was confirmed by the phenotypes: esterase (M2, malate dehydrogenase (N3, superoxide dismutase (N2 and glutamate oxaloacetate transaminase (N3. This is the first report of the M. mayaguensis occurrence in MG. Other nematodes associated with the okra plants were Aphelenchus sp., Criconemella sp., Helicotylenchus spp., Pratylenchus brachyurus and P. coffeae, Rotylenchulus reniformis, Rotylenchus sp., Tylenchus sp. and Tylenchorhynchus sp.

  13. Radioactive seed migration after prostate brachytherapy with Iodine-125 using loose seeds versus stranded seeds

    Carlos A. S. Franca

    2009-10-01

    Full Text Available Objectives: To assess the incidence and clinical parameters that could influence migration of seeds in localized prostate cancer patients treated by stranded versus loose sources by Iodine-125 brachytherapy. Material and Methods: 100 patients were treated from January/1998 until December/2006. Age, PSA, clinical stage, Gleason, prostate volume, number of seeds, activity of radioactive seeds, and dosimetric parameters, such as V100, V150 and D90 were evaluated. Results: Mean follow-up was 79 months (18 - 120. CI 95%: 72 - 85. Overall, 6 of 100 patients experienced seed migration. Seed migration was found in 4/50 (8% patients using loose seeds and in 2/50 (4% treated by stranded seeds. Mean value dosimetric parameters for stranded seeds were greater than those for loose seeds (V100(%: 88.7/82, D90(Gy: 149.2/140.3, D90(%: 104.2/93.8, V150 (%: 53.8/47, respectively. No significant difference in migration of seeds was detected between loose and stranded seeds considering age (p = 0.33, PSA (p = 0.391, prostate volume (p = 0.397, activity of radioactive seeds (p = 0.109, number of seeds (p = 0.338, V100 (p = 0.332, although significant differences were measured in the values of D90(% and Gy (p = 0.022 and 0.011 and V150 (p = 0.023. Conclusions: Seed migration after brachytherapy might occur and it does affect post-implant dosimetry.

  14. Seeds of confusion : the impact of policies on seed systems

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important development issue. This study deals with the impact different types of regulation have on how farmers access seed.  I have analysed current regulatory frameworks in terms of their impact on differ...

  15. Seeds of confusion : the impact of policies on seed systems

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important development issue. This study deals with the impact different types of regulation have on how farmers access seed.  I have analysed current regulatory frameworks in terms of their impact on differ...

  16. Magnetic-seeding filtration

    Depaoli, D. [Oak Ridge National Lab., TN (United States)

    1996-10-01

    This task will investigate the capabilities of magnetic-seeding filtration for the enhanced removal of magnetic and nonmagnetic particulates from liquids. This technology appies to a wide range of liquid wastes, including groundwater, process waters, and tank supernatant. Magnetic-seeding filtration can be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal-size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes.

  17. Healthy food trends -- chia seeds

    ... are looking at whether consuming more of the fatty acids found in chia seeds can improve blood pressure, heart health, blood sugar, or provide other benefits. If you plan to consume chia seeds or ...

  18. COFFEE SEED PHYSIOLOGY

    There are more than 70 species of Coffea (Rubiaceae), but only C. arabica and C. canephora are used commercially. Better understanding of seed physiology within Coffea will facilitate the incorporation of genetic traits for resistance to biotic and abiotic stresses from wild relatives into commerci...

  19. Magnetic-seeding filtration

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process

  20. The SEED Initiative

    Teich, Carolyn R.

    2011-01-01

    Committed to fulfilling the promise of the green economy, the American Association of Community Colleges (AACC) launched the Sustainability Education and Economic Development (SEED) initiative (www.theseedcenter.org) in October 2010. The project advances sustainability and clean energy workforce development practices at community colleges by…

  1. Magnetic-seeding filtration

    DePaoli, D.W.; Tsouris, C. [Oak Ridge National Lab., TN (United States); Yiacoumi, Sotira

    1997-10-01

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process.

  2. Corridors cause differential seed predation.

    JOHN L. ORROCk; ELLEN I. DAMSCHEN

    2005-06-01

    Orrock, John, L., and Ellen I. Damschen. 2005. Corridors cause differential seed predation. Ecol. Apps. 15(3):793-798. Abstract. Corridors that connect disjunct populations are heavily debated in conservation, largely because the effects of corridors have rarely been evaluated by replicated, large-scale studies. Using large-scale experimental landscapes, we found that, in addition to documented positive effects, corridors also have negative impacts on bird-dispersed plants by affecting seed predation, and that overall predation is a function of the seeds? primary consumer (rodents or arthropods). Both large-seeded Prunus serotina and small-seeded Rubus allegheniensis experienced greater predation in connected patches. However, P. serotina experienced significantly less seed predation compared to R. allegheniensis in unconnected patches, due to decreased impacts of rodent seed predators on this large-seeded species. Viewed in light of previous evidence that corridors have beneficial impacts by increasing pollination and seed dispersal, this work demonstrates that corridors may have both positive and negative effects for the same plant species at different life stages. Moreover, these effects may differentially affect plant species within the same community: seeds primarily consumed by rodents suffer less predation in unconnected patches. By shifting the impact of rodent and arthropod seed predators, corridors constructed for plant conservation could lead to shifts in the seed bank.

  3. Tree Seed Technology Training Course: Student Outline.

    Bonner, F. T.; And Others

    This manual is intended primarily to train seed collectors, seed-plant managers, seed analysts, and nursery managers, but can serve as a resource for any training course in forest regeneration. It includes both temperate and tropical tree species of all intended uses and covers the following topics: seed biology, seed collection, seed handling,…

  4. 7 CFR 201.15 - Weed seeds.

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Weed seeds. 201.15 Section 201.15 Agriculture... REGULATIONS Labeling Agricultural Seeds § 201.15 Weed seeds. The percentage of weed seeds shall include seeds of plants considered weeds in the State into which the seed is offered for transportation...

  5. Seed priming to alleviate salinity stress in germinating seeds.

    Ibrahim, Ehab A

    2016-03-15

    Salinity is one of the major abiotic stresses that affect crop production in arid and semiarid areas. Seed germination and seedling growth are the stages most sensitive to salinity. Salt stress causes adverse physiological and biochemical changes in germinating seeds. It can affect the seed germination and stand establishment through osmotic stress, ion-specific effects and oxidative stress. The salinity delays or prevents the seed germination through various factors, such as a reduction in water availability, changes in the mobilization of stored reserves and affecting the structural organization of proteins. Various techniques can improve emergence and stand establishment under salt conditions. One of the most frequently utilized is seed priming. The process of seed priming involves prior exposure to an abiotic stress, making a seed more resistant to future exposure. Seed priming stimulates the pre-germination metabolic processes and makes the seed ready for radicle protrusion. It increases the antioxidant system activity and the repair of membranes. These changes promote seed vigor during germination and emergence under salinity stress. The aim of this paper is to review the recent literature on the response of plants to seed priming under salinity stress. The mechanism of the effect of salinity on seed germination is discussed and the seed priming process is summarized. Physiological, biochemical and molecular changes induced by priming that lead to seed enhancement are covered. Plants' responses to some priming agents under salinity stress are reported based on the best available data. For a great number of crops, little information exists and further research is needed. PMID:26812088

  6. First direct seeding at FLASH

    Maltezopoulos, Theophilos; Azima, Armin; Boedewadt, Joern; Curbis, Francesca; Delsim-Hashemi, Hossein; Drescher, Markus; Hass, Eugen; Hipp, Ulrich; Lechner, Christoph; Miltchev, Velizar; Mittenzwey, Manuel; Rehders, Marie; Roensch-Schulenburg, Juliane; Rossbach, Joerg; Schulz, Michael; Tarkeshian, Roxana; Wieland, Marek [University of Hamburg and CFEL (Germany); Ackermann, Sven; Bajt, Sasa; Duesterer, Stefan; Faatz, Bart; Felber, Matthias; Feldhaus, Josef; Honkavaara, Katja; Laarmann, Tim; Schlarb, Holger; Schreiber, Siegfried; Schroedter, Lasse; Tischer, Markus [DESY, Hamburg (Germany); Ischebeck, Rasmus [PSI, Villigen (Switzerland); Khan, Shaukat [DELTA, Dortmund (Germany)

    2013-07-01

    Direct seeding with a high-harmonic generation source can improve the spectral, temporal, and coherence properties of a free-electron-laser (FEL) and reduces intensity- and arrival-time fluctuations. In the seeding experiment at the XUV-FEL in Hamburg, FLASH, which is normally operated in the self-amplified spontaneous emission mode, the 21st harmonic of an 800 nm laser is focused into a dedicated seeding undulator. The interaction with the relativistic electrons acts as an amplifier for the seed radiation. We present the setup of the seeding section of FLASH and first experimental results.

  7. Irradiation effect on the seed vigor, SOD activity and MDA content in germinating seeds of yellow-seeded and black-seeded rape seed (Brassica napus L.)

    Seeds of a set of near-isogenic lines (Brassica napus L.) with different seed coat color from yellow to black were irradiated by 60Co γ-rays of 150 krad. Seed vigor, superoxide dismutase (SOD) and malondialdehyde (MDA) in germinating seeds were analysed. In these characters, no significant difference between yellow-seeded lines (YLs) and black-seeded lines (BLs) showed before irradiation. But after irradiation, SOD activity in YLs was lower than that in BLs. While MDA content in YLs was obviously higher that that in DLs. As a result of irradiation, seed vigor of YLs was lower than that in BLs. these results indicated that the irradiation resistance of rape seed was related to the level of SOD as well as protective structure or substances in seed coat and that the radiosensitivity of YLs was higher than that of DLs

  8. Breeding for Grass Seed Yield

    Boelt, Birte; Studer, Bruno

    Seed yield is a trait of major interest for many fodder and amenity grass species and has received increasing attention since seed multiplication is economically relevant for novel grass cultivars to compete in the commercial market. Although seed yield is a complex trait and affected by agricult......Seed yield is a trait of major interest for many fodder and amenity grass species and has received increasing attention since seed multiplication is economically relevant for novel grass cultivars to compete in the commercial market. Although seed yield is a complex trait and affected by...... agricultural practices as well as environmental factors, traits related to seed production reveal considerable genetic variation, prerequisite for improvement by direct or indirect selection. This chapter first reports on the biological and physiological basics of the grass reproduction system, then highlights...

  9. Seed longevity and seeding strategies affect sagebrush revegetation

    Booth, D.T. [USDA ARS, Cheyenne, WY (United States)

    2002-07-01

    Three hypotheses were tested relating to Wyoming big sagebrush (Artemisia tridentata Nutt. ssp. wyomingensis (Beetle and Young)) revegetation on coal-mined land in Wyoming: (1) that fourwing saltbush (Atriplex canescens (Pursh) Nutt.) seeded > 2.2 kg pure live seed (pIs)/ha would exclude sagebrush, (2) the contrasting view that the saltbush, as a 'pioneer plant', facilitated sagebrush stand development (by promoting beneficial soil microbiological activity), and (3) that sagebrush stand development would be greater on fresh-stripped, than on stored, topsoil. The hypotheses were tested by comparing stand development on field plots: 1) seeded to sagebrush in February 1992, and March 1993; 2) followed in 1992, and sagebrush seeded in March 1993; 3) seeded to 'Wytana' fourwing saltbush in November, 1991, with sagebrush over-seeded in March, 1993, and; 4) no seeding. The experimental design was a randomized complete block with split plots of stored and fresh-stripped topsoil and with 3 replications. New sagebrush were detected annually through 4 post-seeding spring counts. Seed efficiency was affected by seeding strategy, but efficiency, density, and height were not affected by topsoil source. Proximity to saltbush did not affect sagebrush heights. The results imply that a 'Wytana' density less than or equal to 5 seedlings/m{sup 2} is unlikely to deter or promote development of the sagebrush stand, but it will significantly increase total-shrub seed efficiency and density. Seeding strategies, particularly pre-sowing fallow and mixed-species seedings, will likely have a greater influence on sagebrush revegetation than will topsoil source when topsoils are handled as they were in this study.

  10. SEDA (SEed DAtabase)

    Šerá, Božena

    Praha : Botanická zahrada hl. m. Prahy, 2005 - (Sekerka, P.), s. 64-65 ISBN 80-903697-0-7. [Introdukce a genetické zdroje rostlin. Botanické zahrady v novém ticíciletí. Praha (CZ), 05.09.2005] R&D Projects: GA MŠk(CZ) 1P05OC049 Institutional research plan: CEZ:AV0Z60870520 Keywords : database, seed, diaspore, fruit, Subject RIV: EH - Ecology, Behaviour

  11. Seeds of the Future

    CERN. Geneva

    2015-01-01

    Five of the global issues most frequently debated today are the decline of biodiversity in general and of agrobiodiversity in particular, climate change, hunger and malnutrition, poverty and water. These issues are connected with each other, and should be dealt with as such. Most of our food comes from seeds (even when we eat meat, we indirectly eat plants, which come from seeds) and food affects our health. The evolution of plant breeding, the science which is responsible for the type and the diversity of seed that farmers plant, and hence for the diversity of food that we eat, helps us understand how agrobiodiversity has decreased. An agro-ecological model of agriculture could be solution to the most important problems affecting the planet, but is often criticized for not being able to produce enough food for a growing population casting doubts on whether food security and food safety can be compatible objectives. Participatory and evolutionary plant breeding, while benefiting from advances in molecular g...

  12. Improving soybean seed quality

    Both the meal and oil fractions of soybeans may be genetically improved, either by mutagenesis or by genetic engineering. There are a number of mutant lines of soybeans containing a low raffinosaccharide meal, which can be used for animal feed, with an improved total metabolizable energy content. Mutant lines with an improved fatty acid profile of the oil include high oleic and high stearic soybeans. Cloning of the mutant genes facilitates the integration of these traits into high yielding elite lines by providing molecular markers. Cloned genes may also be reintroduced into soybeans to create transgenic lines with improved meal and oil traits, such as seeds with an increased lysine content and stable soybean oils with a very low content of polyunsaturated fatty acids. The design of transgene constructs has been assisted by using soybean somatic embryos in suspension culture as a model system for soybean seed transformation. This system has allowed selection of those genes and promoters that are the most effective way of achieving the desired phenotypes in soybeans. Experiments with constructs containing fatty acid biosynthesis genes in somatic embryos have also led to the conclusion that, in soybeans, gene-transgene sense suppression is a more effective way of silencing endogenous genes than antisense. Sense suppression of genes encoding microsomal, fatty acid omega-6 desaturates has resulted in soybean lines with over 80% oleic acid in their seed oil, and this trait is stable over at least three generations. (author). 12 refs, 2 figs

  13. Radioactive seed migration after prostate brachytherapy with Iodine-125 using loose seeds versus stranded seeds

    Franca, Carlos A.S.; Vieira, Sergio L.; Bernabe, Antonio J.S.; Penna, Antonio B.R. [Brazilian Institute of Oncology, Rio de Janeiro, RJ (Brazil); Radioterapia Botafogo, Rio de Janeiro, RJ (Brazil)], e-mail: carlosfranca@cremerj.org.br; Carvalho, Antonio C.P. [Federal University of Rio de Janeiro (UFRJ), RJ (Brazil). Clementino Fraga Filho University Hospital

    2009-09-15

    Objectives: To assess the incidence and clinical parameters that could influence migration of seeds in localized prostate cancer patients treated by stranded versus loose sources by Iodine-125 brachytherapy. Materials and Methods: 100 patients were treated from January/1998 until December/2006. Age, PSA, clinical stage, Gleason, prostate volume, number of seeds, activity of radioactive seeds, and dosimetric parameters, such as V{sub 100}, V{sub 150} and D{sub 90} were evaluated. Results: Mean follow-up was 79 months (18 - 120. CI 95%: 72 - 85). Overall, 6 of 100 patients experienced seed migration. Seed migration was found in 4/50 (8%) patients using loose seeds and in 2/50 (4%) treated by stranded seeds. Mean value dosimetric parameters for stranded seeds were greater than those for loose seeds (V{sub 100}(%): 88.7/82, D{sub 90}(Gy): 149.2/140.3, D{sub 90}(%): 104.2/93.8, V{sub 150} (%): 53.8/47, respectively). No significant difference in migration of seeds was detected between loose and stranded seeds considering age (p = 0.33), PSA (p = 0.391), prostate volume (p 0.397), activity of radioactive seeds (p = 0.109), number of seeds (p 0.338), V{sub 100} (p = 0.332), although significant differences were measured in the values of D{sub 90} (% and Gy) (p = 0.022 and 0.011) and V{sub 150} (p = 0.023). Conclusions: Seed migration after brachytherapy might occur and it does affect post-implant dosimetry. (author)

  14. Physicochemical Evaluation of Seeds and Oil of Nontraditional Oil Seeds

    Adam Ismail Ahmed

    2015-08-01

    Full Text Available The present work was conducted in the Laboratory of Biochemistry and Food science department, Faculty of Natural Resources and Environmental Studies, University of Kordofan, in order to evaluate some nontraditional oil seeds these are i.e. Marula (Sclerocarya birrea, Roselle (Hibiscus sabdariffa L. seeds and Christ’s thorn (Zizyphus spina-christi seeds. The seeds of the roselle and Christ’s thorn fruits were procured from Elobeid local market, North Kordofan State, while marula fruits were obtained from Elnuhod, West Kordofan State. The proximate composition of the seeds, cake and christ’s thorn pulp was done. Some chemical and physical properties were performed for the extracted oil. The results revealed that proximate composition of the seeds and cake differ statistically among the studied materials. Significant differences were observed among the oil extracted from these species; moreover, these oils differ significantly in color and viscosity only.

  15. IMPACT OF IMPROVED SEEDS ON SMALL FARMERS' PRODUCTIVITY, INCOME AND LIVELIHOOD OF BARA LOCALITY IN NORTH KORDOFAN STATE, SUDAN

    Elkhalil Elnour Briema Ahmed

    2013-08-01

    Full Text Available This study was designed to test and identify the impact of improved seeds on small farmers' productivity, income and livelihood in Bara locality. Sixty households participants were randomly selected through a field survey during 2011 for 2008/2009, 2009/2010 and 2010/2011 cropping seasons. The study applied Multi-stage random sample technique. Based on existing farm situation and price level, the sampled farmers were obtained SDG 8604 as gross margin to cover all expenses. Results of this study also depicted that the required net income and off-farm income were 16293 and 11378 SDG, respectively. With respect to Linear Programming (LP results, a total of SDG 8890 were obtained and all crops were entered and solved. The optimal plan and existing farm situation were changed by 3.3 and 5.6% for gross margin and cash income, respectively. Results of LP also indicated a positive change in production patterns of resource use; 3.3, 6.2, 3.5, 3.3 and 9.1% for land, cash income, labour, seeds supply and productivity, respectively under existing and optimal plan. Partial crop budgeting revealed that, all treatments were financially gave positive returns. Dominance analysis showed that cowpea ainelgazal, okra, roselle and sesame herhri crops were dominated by crops of millet ashana, watermelon, groundnut and guar, respectively. Marginal analysis exposed that, for every SDG 1.00 invested in improved seeds cultivation, farmer can expect to cover the SDG 1.00 and obtain an additional SDG 1.345; then, additional seed rate implies a further marginal rate of SDG 43.9. Sensitivity analysis for cost over run and benefit reduction by 10% indicated highly stability with MRR of 1.22, 3.991 and 1.21 and 3.951% for watermelon and guar, respectively. The productivity of improved seeds compared to local ones was increased in some varieties and decreased among others. This study reached to some recommendations for improving crop productivity, production and livelihood of small farmers in Bara locality.

  16. Seed storage behavior of forest tree species seeds

    Marcela Carlota Nery

    2014-09-01

    Full Text Available Seeds of five forest species were classified according to their physiological storage behavior. Seeds of Casearia sylvestris Swart (Salicaceae, Qualea grandiflora Mart. (Vochysiaceae, Guarea kunthiana A. Juss. (Meliaceae, Eremanthus incanus Less. (Asteraceae, Protium heptaphyllum March. (Burseraceae were collected and taken to the laboratory, where they were processed and submitted to both rapid and slow drying, storage and assayed for viability. After physiological classification regarding storage behavior, it was observed that seeds of C. sylvestris and E. incanus presented orthodox behavior. Seeds of G. kunthiana and P. heptaphyllum were classified as recalcitrant and Q. grandiflora as an intermediate, which did not tolerate low moisture content.

  17. Differential seed handling by two African primates affects seed fate and establishment of large-seeded trees

    Gross-Camp, Nicole D.; Kaplin, Beth A.

    2011-11-01

    We examined the influence of seed handling by two semi-terrestrial African forest primates, chimpanzees ( Pan troglodytes) and l'Hoest's monkeys ( Cercopithecus lhoesti), on the fate of large-seeded tree species in an afromontane forest. Chimpanzees and l'Hoest's monkeys dispersed eleven seed species over one year, with quantity and quality of dispersal varying through time. Primates differed in their seed handling behaviors with chimpanzees defecating large seeds (>0.5 cm) significantly more than l'Hoest's. Furthermore, they exhibited different oral-processing techniques with chimpanzees discarding wadges containing many seeds and l'Hoest's monkeys spitting single seeds. A PCA examined the relationship between microhabitat characteristics and the site where primates deposited seeds. The first two components explained almost half of the observed variation. Microhabitat characteristics associated with sites where seeds were defecated had little overlap with those characteristics describing where spit seeds arrived, suggesting that seed handling in part determines the location where seeds are deposited. We monitored a total of 552 seed depositions through time, recording seed persistence, germination, and establishment. Defecations were deposited significantly farther from an adult conspecific than orally-discarded seeds where they experienced the greatest persistence but poorest establishment. In contrast, spit seeds were deposited closest to an adult conspecific but experienced the highest seed establishment rates. We used experimental plots to examine the relationship between seed handling, deposition site, and seed fate. We found a significant difference in seed handling and fate, with undispersed seeds in whole fruits experiencing the lowest establishment rates. Seed germination differed by habitat type with open forest experiencing the highest rates of germination. Our results highlight the relationship between primate seed handling and deposition site and seed fate, and may be helpful in developing models to predict seed shadows and recruitment patterns of large-seeded trees.

  18. Pathogenic mycoflora on carrot seeds

    Bogdan Nowicki

    2013-12-01

    Full Text Available Altogether 300 seed samples were collected during 9 years in 8 regions of Poland and the fungi Were isolated and their pathogenicity to carrot seedlings was examined. Alternaria rudicina provcd to be the most important pathogen although. A. alternata was more common. The other important pathogens were Fusarium spp., Phoma spp. and Botrytis cinerea. The infection of carrot seeds by A. radicina should be used as an important criterium in seed quality evaluation.

  19. 7 CFR 201.50 - Weed seed.

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Weed seed. 201.50 Section 201.50 Agriculture... REGULATIONS Purity Analysis in the Administration of the Act § 201.50 Weed seed. Seeds (including bulblets or tubers) of plants shall be considered weed seeds when recognized as weed seeds by the law or rules...

  20. Seed to Seed, The Secret Life of Plants

    This is a book review of "Seed to Seed, The Secret Life of Plants", by Nicholas Harberd. The book is a diary of ruminations/introspections about plant development and about the main research topic of Nick Harberd’s laboratory. His intended audience is initially nonscientists; many of the explanation...

  1. 19 CFR 10.57 - Certified seed potatoes, and seed corn or maize.

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Certified seed potatoes, and seed corn or maize... Provisions Potatoes, Corn, Or Maize § 10.57 Certified seed potatoes, and seed corn or maize. Claim for classification as seed potatoes under subheading 0701.10.00, as seed corn (maize) under subheading...

  2. Effect of Intercropping Okra (Hibiscus esclentus with Pumpkin (Curcubita maxima Dutch ex Lam on Some Growth Parameters and Economic Yield of Maize (Zea mays and Maximization of Land Use in a Fadama Soil

    2006-01-01

    Full Text Available There is a need to harness fadama lands in Niger-delta as a result of dearth of fertile arable land due to soil infertility from little or no fallow period. This study was carried out, to determine the compatibility of okra, pumpkin and maize in a fadama soil. Oba super2 variety of maize, NHae 47-4 variety of okra and locally sourced pumpkin were sown in October, 2003 and repeated in October 2004, in a randomized complete block design with 4 replicates. Maize and okra were monitored weekly for 8 and 10 weeks respectively for plant height and number of leaves while pumpkin were accessed for % vine coverage. Observation were carried for weed biomass, economic yield, aggregate yield and land equivalent ratio were calculated from the economic yield. The result of the 2 year study showed, that growth parameters did not all follow a consistend trend, although sole crops performed better than in their crop associations. The presence of pumpkin significantly reduced weeds growth in the crop associations. The economic or relative economic yield was significantly higher (p<0.05 in sole crops than in the individual crops of the association, but the aggregate yield was higher in the crop association than in their sole crops plot. The land equivalent ratio was highest in 3 crop association (1.97 than 2 crop association and least in sole crops of 1.00.

  3. Seed production and quality of pinus durangensis mart., from seed areas and a seed stand in durango, mexico

    Seed productive potential, production efficiency and seed quality of seed areas of P. durangensis Mart. from La Florida and La Campana, and from a Pericos seed stand, located in Durango state, Mexico were investigated. The productive potential, developed seeds, upper and lower infertile ovules, and aborted ovules during the first and second year of seed formation were determined. X-ray scanning was used to determine the percentage of seeds that were filled, emptied, malformed, or damaged by insects. Seed production efficiency was also determined. Speed, value and percentage of germination were determined under laboratory conditions. The Pericos seed stand had the greatest seed productive potential, in terms of number of developed seeds per cone (100.3) and filled seeds (79.7), and in the percentage of filled seeds (73.4%) and seed germination (53.8%). The Pericos seed stand had the highest seed production efficiency (57.6%); this low efficiency reflects problems of damage caused by insects and deficient management. The highest ovule abortion during the first year was observed at La Campana seed area, due to self-pollination or damage by the seedbug Leptoglossus occidentalis in the early stages of seed development. (author)

  4. A guide to forest seed handling

    Willan, R.L.

    1986-01-01

    This guide to forest seed handling focuses on seed quality, i.e., the physiological viability and vigor of the seeds. Seed and fruit development, germination, and dormancy and the fundamentals of planning seed collections are covered. The guide includes discussions on seed collection of fallen fruits or seeds from the forest floor from the crowns of felled trees, and from standing trees with access from the ground and with other means of access. Also considered are precautions to be followed during fruit and seed handling between collection and processing. The different stages in seed processing are detailed, including extraction, depulping, drying, tumbling and threshing, dewinging, cleaning, grading, and mixing. Factors affecting seed longevity in storage and the choice of storage methods are reviewed. Different forms of seed pretreatment and seed testing methods are described.

  5. Insecticide seed treatments for sugarbeet

    Pest feeding and vectoring of viruses cause serious problems in sugarbeet production worldwide. In order to ameliorate pest and disease problems on sugarbeet, two seed treatments, Poncho Beta (60 g a.i. clothianidin + 8 g a.i. beta-cyfluthrin/100,000 seed) and Cruiser Tef (60 g a.i. thiamethoxam + 8...

  6. Efficient computation of spaced seeds

    Ilie Silvana

    2012-02-01

    Full Text Available Abstract Background The most frequently used tools in bioinformatics are those searching for similarities, or local alignments, between biological sequences. Since the exact dynamic programming algorithm is quadratic, linear-time heuristics such as BLAST are used. Spaced seeds are much more sensitive than the consecutive seed of BLAST and using several seeds represents the current state of the art in approximate search for biological sequences. The most important aspect is computing highly sensitive seeds. Since the problem seems hard, heuristic algorithms are used. The leading software in the common Bernoulli model is the SpEED program. Findings SpEED uses a hill climbing method based on the overlap complexity heuristic. We propose a new algorithm for this heuristic that improves its speed by over one order of magnitude. We use the new implementation to compute improved seeds for several software programs. We compute as well multiple seeds of the same weight as MegaBLAST, that greatly improve its sensitivity. Conclusion Multiple spaced seeds are being successfully used in bioinformatics software programs. Enabling researchers to compute very fast high quality seeds will help expanding the range of their applications.

  7. STORAGE OF Handroanthus umbellatus SEEDS

    Cibele Chalita Martins

    2014-09-01

    Full Text Available http://dx.doi.org/10.5902/1980509815725Seed storage under controlled environmental conditions represents one of the most important lines of research to be applied on short-lived forest species as Handroanthus. The present research aimed to identify the most suitable seed storage conditions and longevity behavior of Handroanthus umbellatus seeds subject to the following storage treatments: packaging permeable paper bags under a no-controlled laboratory temperature and humidity (control and multiwall semipermeable bag at temperatures of -18 ºC, 1 ºC and 25 ºC. Seeds were dried to 6.3% of water content. Stored seeds were evaluated every three months until 24 months for water content, germination percentage and vigor utilizing first counting test. Seeds of T. umbellata are orthodox, with low longevity under natural conditions, once they remain viable for less than 5 months. The best conditions of seed preservation of these species were obtained by storage at -18° C in multiwall bags. Under these conditions physiological seed quality remains unchanged for a 24-month period.

  8. Tetrazolium test in timbaúba seeds

    Narjara Walessa Nogueira

    2014-12-01

    Full Text Available The tetrazolium test has shown itself to be a promising alternative in determining the viability and vigor of seeds of forest species by the quality and speed of data collection. Thus, this paper was developed with the goal of standardizing the methodology for conducting the tetrazolium test for assessing the physiological quality of seed lots Timbaúba. To evaluate the physiological quality of seeds the four lots were subjected to the following determinations: water content, germination, first count and speed germination. Four replicates of 25 seeds from each batch were submerged in tetrazolium solution and kept in the dark at 35 °C for periods of 1, 3 and 6 hours under concentrations of 0.1, 0.075 and 0.050%. After the staining periods, the seeds were washed, split lengthwise between the cotyledon and ranked in viable and not viable. Seed exposure for periods of one hour and six hours has proved inadequate for driving the tetrazolium test since not promoted the proper interpretation of staining results. The tetrazolium test is a viable alternative for the evaluation of existing lots of Timbaúba. To conduct the test recommended scarification followed by soaking the seeds in water for 24 hours and coat removal, with subsequent soaking of seeds for three hours in tetrazolium solution of 0.075 % at 35 ºC.

  9. Brassinosteroid functions in Arabidopsis seed development

    Jiang, Wen-Bo; Lin, Wen-Hui

    2013-01-01

    Seed development of flowering plant is a complicated process controlled by a signal network. Double fertilization generates 2 zygotic products (embryo and endosperm). Embryo gives rise to a daughter plant while endosperm provides nutrients for embryo during embryogenesis and germination. Seed coat differentiates from maternally derived integument and encloses embryo and endosperm. Seed size/mass and number comprise final seed yield, and seed shape also contributes to seed development and weig...

  10. Seed coat thickness data clarifies seed size-seed persistence tradeoffs in Abutilon theophrasti (Malvaceae)

    Theoretical models predict that seed size and seedbank persistence evolve interdependently such that strong selection for one trait corresponds with weak selection for the other. This framework is supported by empirical data but conclusive evidence is lacking. In this study, we expand the seed size-...

  11. A system for generating virtual seeds

    Sako Y.

    1998-01-01

    Full Text Available Seed analysts need to identify seeds, and seed catalogs are used as a reference to accomplish this task. Conventional seed catalogs supply two-dimensional photographs and hand-drawn diagrams. In this study, a new, three-dimensional representation of seeds is developed to supplement these traditional photographs and drawings. QuickTime VR is a promising method for viewing three-dimensional objects on a computer screen. It permits manipulation of an object by rotating and viewing it from any pre-specified angle at an interactive speed, allowing the viewer the sense of examining a hand-held object. In this study, QuickTime VR object movies of seeds were created as interactive "movies" of seeds that can be rotated and scaled to give the viewer the sensation of examining actual seeds. This approach allows the examination of virtual seeds from any angle, permitting more accurate identification of seeds by seed analysts.

  12. A novel postharvest rot of okra pods caused by Rhizoctonia solani in Brazil Uma nova podridão pós-colheita de frutos de quiabo causada por Rhizoctonia solani

    Gilmar P. Henz

    2007-06-01

    Full Text Available Okra pods with unusual brown lesions and rot were collected in a local supermarket in Brasília DF. The objective of this paper was to characterize the causal agent, to fulfill Koch's postulates and to determine some conditions conducive to disease. The pathogen was identified as Rhizoctonia solani based on morphological characteristics which fitted the fungus description, such as pale to brown hyphae, with nearly right-angled side branches constricted at the base, hyphal cells 6-10 µm wide with a septum near the base. Five isolates were obtained from infected pods and identified as AG 1-IB anastomosis group. Wounded or unwounded okra pods cv. Santa Cruz 47 were inoculated with mycelium disks of R. solani and kept in humid chambers at 12 ºC or 25 ºC. After seven days at 25 ºC, both wounded and unwounded pods were completely rotted and brown, while those kept at 12 ºC showed small lesions ranging from 0.6 to 1.0 mm only in wounded pods. The pathogen was able to grow in different materials used for assembling crates and packs of horticultural products, such as pinewood, corrugated carton, plastic, Styrofoam and newspaper sheets when kept in humid chambers (24 ºC, 96 % RH. The disease occurrence can be related to careless handling practices and to the transmission of R. solani propagules by infected plant debris or soil particles. This is the first report of Rhizoctonia solani causing postharvest rot in okra pods in Brazil.Frutos de quiabo apresentando podridão e lesões marrons foram coletados em um supermercado de Brasília DF. O objetivo deste trabalho foi identificar o agente causal e comprovar o envolvimento do fungo como causador da doença (Postulados de Koch e determinar algumas condições favoráveis à ocorrência da doença em frutos de quiabo após a colheita. O patógeno foi identificado como Rhizoctonia solani baseado nas suas características morfológicas, como hifas marrons a ocre, com ramificações laterais em ângulos quase retos com constrições na base, células da hifa com 6-10 µm de largura com um septo perto da base. Cinco isolados foram obtidos dos frutos infectados e identificados como sendo do grupo de anastomose AG 1-IB. Frutos de quiabo cv. Santa Cruz 47 inoculados com discos de micélio de R. solani com e sem ferimentos e mantidos em câmaras úmidas, a 25 ºC, por sete dias ficaram completamente apodrecidos pelo patógeno, com cor marrom, enquanto somente os frutos com ferimentos mantidos a 12 ºC apresentaram lesões pequenas, variando de 0,6 a 1,0 mm de diâmetro. Em outro experimento, foi demonstrado que o patógeno foi capaz de crescer na superfície de diferentes materiais usados na confecção de embalagens de produtos hortícolas, como madeira de pinus, papelão corrugado, plástico, isopor e folhas de jornal mantidos em câmara úmida (24 ºC, 96 % UR. A ocorrência da doença está relacionada com manuseio pós-colheita inadequado, e a transmissão de propágulos do fungo junto com restos culturais ou partículas de solo. Este é o primeiro relato de R. solani causando podridão pós-colheita em frutos de quiabo no Brasil.

  13. Organic Upland Rice Seed Production

    Raumjit Nokkoul

    2014-12-01

    Full Text Available The upland rice is popular for growing in southern Thailand because this area is the lowland and less area than other sectors. Upland rice is grown as alternative crops of farmers for household consumption which using organic farming method because organic rice seed can be produced by self-production in farmhouse. However, the upland rice is grown under organic farming system. The seeds must originate from plants being grown in compliance with the organic farming rules for at least one generation. There are many factors involving the production of seeds under organic farming system, making the yield low. Thus, the objective of this study on appropriate methods of upland rice seed production under organic farming system in southern Thailand. The results showed that in producing organic seeds, suitable varieties should be selected to suit each area with regular high yield quality. It can be grown in low fertile soil, resist pests and diseases and compete with weeds. The suitable season should be selected for the seed production and the growing areas ought to be in an ecological zone with at least 14-20 mm of 5-day rainfall during the growing cycle. Soil fertility: crop rotation, green manure plants, compost of rice straw and organic manures. For control of disease and insect pests use of resistant or tolerant varieties, plant extracts, natural enemies. The organic seed production of upland rice in southern Thailand, Samduen variety had suitability for recommendation to seed producer in this area because it can provide high growth, yield and seed quality.

  14. Laser treatment of radish seed

    Trials were conducted in unheated plastic greenhouses in 1985-1986 to test the effect of laser treatment on radish seed. Seed of cv Saxia was irradiated with helio-neon laser of 632.8 Nm wave length at: 2-, 4- and 6-fold irradiation. Results showed that plants of all variants emerged almost simultaneously. The root mass was greatest for plants obtained from 4-fold irradiated seed. Treatment enhanced root production in 1985, and in 1986 4-fold irradiation boosted yield by 15%

  15. Materials used for Seed Storage Containers: Response

    Efficient seed storage is a shared concern among the growing number of seed banks established for crop improvement or ex situ conservation. Container properties greatly affect seed interactions with the environment and the overall cost and success of seed banking operations. Several material proper...

  16. Relationship between eggplant seed morphology and germination

    Vanessa Neumann Silva

    2012-01-01

    Full Text Available Structural differences such as abnormalities, damage and free spaces in seeds may affect germination. The aim of this study was to study the relationship between eggplant seed morphology and seed germination. Ten seed lots of the eggplant cultivar Embu were evaluated by X-ray image analysis and the germination test. Seed image analysis was performed by Image Pro Plus® software and the whole seed area and free space between the embryo and endosperm were measured. The internal seed area filled by the embryo and endosperm was calculated from the difference between the whole seed and free space areas. Based on these results and visual seed analysis, seeds were classified into three categories and information on germination was obtained for each one. X-ray image analysis provides a perfect view of the internal seed parts and for seed morphology studies. An increase in seed area filled by the endosperm and embryo does not improve seed germination. Mechanical seed damage and deteriorated tissues can adversely affect seed germination.

  17. Autoradiography for iodine-125 seeds

    To study the interior design of model 6702 and 6711 iodine-125 seeds, contact autoradiographs were performed using mammography film. Improved resolution was obtained using a pin-hole camera with a hole of 0.1 mm x 0.1 mm. With these techniques, qualitative determination of the relative activity distribution within each seed was possible. The number of the activated resin spheres and the positions of the centers of these spheres can be exactly determined. A model calculation shows that variations in the arrangement of the activated spheres within a seed have a moderate influence on the dose distribution at source distances below 10 mm. Knowing the exact source configuration may be useful when comparing dose calculations with measured data for model 6702 125I seeds which are currently employed in ophthalmic plaque and implant therapy of other tumors. 16 refs., 5 figs., 2 tabs

  18. Seeding for pervasively overlapping communities

    Lee, Conrad; McDaid, Aaron; Hurley, Neil

    2011-01-01

    In some social and biological networks, the majority of nodes belong to multiple communities. It has recently been shown that a number of the algorithms that are designed to detect overlapping communities do not perform well in such highly overlapping settings. Here, we consider one class of these algorithms, those which optimize a local fitness measure, typically by using a greedy heuristic to expand a seed into a community. We perform synthetic benchmarks which indicate that an appropriate seeding strategy becomes increasingly important as the extent of community overlap increases. We find that distinct cliques provide the best seeds. We find further support for this seeding strategy with benchmarks on a Facebook network and the yeast interactome.

  19. Kauri seeds and larval somersaults

    Dupont, Steen Thorleif

    2012-01-01

    The trunk morphology of the larvae of the kauri pine (Agathis) seed infesting moth Agathiphaga is described using conventional, polarization, and scanning electron microscopy. The pine seed chamber formed by the larva is also described and commented on. The simple larval chaetotaxy includes more of...... the minute posture sensing setae, proprioceptors, than expected from the lepidopteran larval ground plan. The excess of proprioceptors is suggested to be necessary for sensory input concerning the larval posture within the seed chamber. The trunk musculature includes an autapomorphic radial ventral...... musculature made up of unique multisegmental muscles. The combined presence of additional proprioceptors and the unique ventral musculature is proposed to be related to the larval movement within the confined space of the seed chamber, especially to a proposed somersault movement that allows the larva to...

  20. Effect of seed rate and row spacing in seed production of Festulolium

    Deleuran, L C; Gislum, R; Boelt, B

    2010-01-01

    seed yield. No effect of row spacing on seed yield was observed in Paulita. Neither of the two types was affected in seed yield by seed rate. The fact that row spacing in both types can be increased to 24 cm without having a concomitant negative effect on seed yield implies that mechanical weed control...

  1. Diamond Synthesis Employing Nanoparticle Seeds

    Uppireddi, Kishore (Inventor); Morell, Gerardo (Inventor); Weiner, Brad R. (Inventor)

    2014-01-01

    Iron nanoparticles were employed to induce the synthesis of diamond on molybdenum, silicon, and quartz substrates. Diamond films were grown using conventional conditions for diamond synthesis by hot filament chemical vapor deposition, except that dispersed iron oxide nanoparticles replaced the seeding. This approach to diamond induction can be combined with dip pen nanolithography for the selective deposition of diamond and diamond patterning while avoiding surface damage associated to diamond-seeding methods.

  2. Seed Coat Permeability of Active Ingredients

    Niemann, Sylvia

    2013-01-01

    The seed coat is the barrier controlling exchange of solutes between the plant embryo and its environment. This exchange is of importance for example in the uptake of germination inhibitors or in the uptake of agrochemicals applied as seed treatment. A thorough understanding of the basic mechanisms underlying solute permeation across the seed coat would help to improve the effectiveness of seed treatment formulations. In seed treatment formulations, additives can be used to enhance or decreas...

  3. Local Evolution of Seed Flotation in Arabidopsis

    Saez Aguayo, S.; Rondeau Mouro, C.; Macquet, A.; Kronholm, I.; Ralet, M.C.; Berger, A; Sallé, C.; Poulain, D.; Granier, F.; Loudet, O.; De Meaux, J.; Marion-Poll, A.; North, H.; Botran, L.

    2014-01-01

    Arabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilage release and float. Four of these have a novel mucilage phenotype with almost none of the released mucilage adhering to the seed and the absence of cellulose microfibrils. Mucilage release was mod...

  4. Gene expression analysis of flax seed development

    Sharpe Andrew; Bekkaoui Faouzi; Deyholos Michael; Cloutier Sylvie; Nowak Jacek; Alting-Mees Michelle; Cram Dustin; Tibiche Chabane; Stone Sandra L; Qiu Shuqing; Xiang Daoquan; Venglat Prakash; Wang Edwin; Rowland Gordon; Selvaraj Gopalan

    2011-01-01

    Abstract Background Flax, Linum usitatissimum L., is an important crop whose seed oil and stem fiber have multiple industrial applications. Flax seeds are also well-known for their nutritional attributes, viz., omega-3 fatty acids in the oil and lignans and mucilage from the seed coat. In spite of the importance of this crop, there are few molecular resources that can be utilized toward improving seed traits. Here, we describe flax embryo and seed development and generation of comprehensive g...

  5. Gene expression analysis of flax seed development

    Venglat, Prakash; Xiang, Daoquan; Qiu, Shuqing; Stone, Sandra L; Tibiche, Chabane; Cram, Dustin; Alting-Mees, Michelle; Nowak, Jacek; Cloutier, Sylvie; Deyholos, Michael; Bekkaoui, Faouzi; Sharpe, Andrew; Wang, Edwin; Rowland, Gordon; Selvaraj, Gopalan

    2011-01-01

    Background Flax, Linum usitatissimum L., is an important crop whose seed oil and stem fiber have multiple industrial applications. Flax seeds are also well-known for their nutritional attributes, viz., omega-3 fatty acids in the oil and lignans and mucilage from the seed coat. In spite of the importance of this crop, there are few molecular resources that can be utilized toward improving seed traits. Here, we describe flax embryo and seed development and generation of comprehensive genomic re...

  6. Evaluation of antioxidant and antiradical properties of Pomegranate (Punica granatum L.) seed and defatted seed extracts

    Basiri, Shadi

    2013-01-01

    Pomegranate seeds are byproducts of the Pomegranate juice industries that contains functional compounds such as phenols. This study was aimed to evaluate the effect of solvents on extraction from Pomegranate seed and Pomegranate defatted seed and to measure the yield extract and phenolic content and antioxidant properties. For this purpose, the seeds and defatted seeds were directly isolated from fruits and seeds by cold pressing respectively, then were crushed and extracted with different so...

  7. Clone variation of seed traits, germination and seedling growth in Dalbergia sissoo Roxb. clonal seed orchard

    O. Singh

    2011-11-01

    Full Text Available A clonal seed orchard (CSO of Dalbergia sissoo Roxb. at Hoshiarpur, India consisting of 20 clones originating from different agro-climatic conditions of four northern states (Uttar Pradesh, Rajasthan, Haryana and Uttarakhand was the source of seeds for variability studies. There was lot of variation in seed size, seed weight, germination percent, germination value and growth rate in nursery of different clones over the years. Seed length, seed width and seed weight were positively correlated to each other but seed size had no effect on germination percent and germination value under laboratory conditions. However, seed weight was found positively correlated with germination percent in nursery with the seed lot of 2008 collection. The genetic parameters for seed traits and seedling growth also showed a wide range of variations in the orchard clones. Heritability values were found to be over 50 percent for seed weight and seed length. However, only seed weight showed high heritability value coupled with more genetic gain across the years, which indicate the presence of good amount of heritable additive component in seed weight. There was no consistency in the seed characters, germination and seedling growth parameters studied across the two years. Effect of clones was dominant and accounted for variation in seed size, seed weight, seed germination and growth parameters. Seed size or seed weight should not be used as criteria for grading of bulked seed lots of different clones, as it can narrow down genetic diversity by rejecting small seeds. The impact of these genetic differences in handling of seed lots during bulking and grading for mass propagation of nursery planting stock of D. sissoo is also discussed.

  8. Neutron irradiation of seeds 2

    The irradiation of seeds with the fast neutron of research reactors has been hampered by difficulties in accurately measuring dose and in obtaining repeatable and comparable results. Co-ordinated research under an international program organized by the FAO and IAEA has already resulted in significant improvements in methods of exposing seeds in research reactors and in obtaining accurate dosimetry. This has been accomplished by the development of a standard reactor facility for the neutron irradiation of seeds and standard methods for determining fast-neutron dose and the biological response after irradiation. In this program various divisions of the IAEA and the Joint FAO/IAEA Division co-operate with a number of research institutes and reactor centres throughout the world. Results of the preliminary experiments were reported in Technical Reports Series No. 76, ''Neutron Irradiation of Seeds''. This volume contains the proceedings of a meeting of co-operators in the FAO/IAEA Neutron Seed Irradiation Program and other active scientists in this field. The meeting was held in Vienna from 11 to 15 December 1967. Refs, figs and tabs

  9. Laser Phase Errors in Seeded FELs

    Ratner, D.; Fry, A.; Stupakov, G.; White, W.; /SLAC

    2012-03-28

    Harmonic seeding of free electron lasers has attracted significant attention from the promise of transform-limited pulses in the soft X-ray region. Harmonic multiplication schemes extend seeding to shorter wavelengths, but also amplify the spectral phase errors of the initial seed laser, and may degrade the pulse quality. In this paper we consider the effect of seed laser phase errors in high gain harmonic generation and echo-enabled harmonic generation. We use simulations to confirm analytical results for the case of linearly chirped seed lasers, and extend the results for arbitrary seed laser envelope and phase.

  10. The future prospects of electronic seed lists:

    Van den Wollenberg, L.J.W.

    2011-01-01

    For many botanic gardens worldwide, seed lists have provided a cost-effective means to replace plant losses in their collections for many decades, if not centuries. In seed lists, seeds are offered on the basis of what each garden can manage to collect and considers ‘of interest’ to other botanic gardens, some offering relatively few, while others offer many, or only wild collected seeds. Seed lists have always been printed on paper. With the use of printed seed lists, the amount of informati...

  11. Cereal seed mycopopulations in Serbia

    Bagi Ferenc F.

    2005-01-01

    Full Text Available Mycopopulation of cereals-durum wheat (cultivars Žitka and Durumko triticale (cultivars Goranac and NS tritikale, winter barley (cultivars NS 131, ZA 37 and Jagodinac and oat (cultivars Slavuj and Rajac had been studied during three years (harvest 2002, 2003 and 2004 from numerous Serbian localities. In all three investigated years and four cereal species the predominant fungal genus were Alternaria and Fusarium. On seeds there were determinated representatives of genus Penicillium, Mucor, Bipolaris Aspergillus, Stemphylium and Epicoccum, too. The established fungi can significantly affect quality of seeds and flour products.

  12. Seed technology training in the year 2000

    McDonald M.B.

    1998-01-01

    Full Text Available Seed quality will remain the centerpiece of successful agricultural programs in the year 2000. As new changes occur in agriculture driven by advancements in biotechnology, seed enhancement technologies, a more diverse seed user clientele, and communication technologies, successful seed companies will require a knowledgeable and informed workforce to assure high seed quality. A new approach to seed technology training is professed that relies on the establishment of a three-institution consortium to achieve this objective. Advantages of the consortium are identified that emphasize the unique strengths of each institution, their geographic advantages representing major climactic/agricultural zones in the world, and differing approaches to seed technology training that are facilitated by increasing ease of global communication. This may be a better way to conduct seed technology training in the year 2000.

  13. Micronutrient application through seed treatments: a review

    M Farooq

    2012-01-01

    Full Text Available Micronutrients are vital for plant growth and human health. Soil and foliar applications are the most prevalent methods of micronutrient addition but the cost involved and difficulty in obtaining high quality micronutrient fertilizers are major concerns with these in developing countries. Micronutrient seed treatments, which include seed priming and seed coating, are an attractive and easy alternative. Here in this review, we discuss the potential of micronutrient seed treatments for improving crop growth and grain nutrient enrichment. Micronutrient application through seed treatments improves the stand establishment, advances phenological events, and increases yield and micronutrient grain contents in most cases. In some instances, seed treatments are not beneficial; however, the negative effects are rare. In most cases, micronutrient application through seed treatment performed better or similar to other application methods. Being an easy and cost effective method of micronutrient application, seed treatments offer an attractive option for resource-poor farmers.

  14. Composition of jojoba seeds and foliage

    Verbiscar, A.J.; Banigan, T.F.

    1978-01-01

    The desert shrub jojoba (Simmondsia chinensis) may be browsed by cattle. The seeds have about 50% oil but the extracted meal is at present unsuitable for feeding. Simmondsin, the most prevalent toxin, is present in seed, 2.3%, and in husks, leaves and twigs. Seeds contained another toxin, Simmondsin 2'-ferulate. The contents of oil, protein, carbohydrate and amino acids in seed are tabulated. 13 references.

  15. Drug Plant Seed Viability Preservation by Cryoconservation

    Alexandra Sh. Dodonova

    2013-01-01

    Full Text Available The article considers the possibility of cryopreservation of seeds of several species of drug plants in Central Kazakhstan – Tanacetum ulutavicum, Niedzwedzkia semiretschenskia, Rhaponticum carthamoides. To increase the amount of viable seeds after liquid nitrogen freezing, we used different defrosting temperatures, deposited seeds with different moisture contents and used different containers for cryopreservation. Recommendations, concerning conditions of cryopreservation of seeds of these drug plant species were developed, basing on the obtained results.

  16. Water uptake curve in physic nut seeds

    Alex Caetano Pimenta; Katia Christina Zuffellato-Ribas; Bruno Galvêas Laviola

    2014-01-01

    Physic nut (Jatropha curcas L.) is a useful species in oil production for energy purposes, however its agricultural crop counts on little scientific knowledge. This work aimed to determine the water uptake curve in physic nut seeds, with the purpose to help future researches depending on this information. Two batches of seeds were used, represented by two sub-samples (intact seeds and dead seeds) of each batch. The water uptake curve was determined weighing samples at specified intervals of t...

  17. Analysis of Seed Potato Systems in Ethiopia

    Hirpa, A.; Meuwissen, M.P.M.; Tesfaye, A.; Lommen, W.J.M.; A.G.J.M. Oude Lansink; Tsegaye, A; Struik, P. C.

    2010-01-01

    This study aimed to analyze the seed potato systems in Ethiopia, identify constraints and prioritize improvement options, combining desk research, rapid appraisal and formal surveys, expert elicitation, field observations and local knowledge. In Ethiopia, informal, alternative and formal seed systems co-exist. The informal system, with low quality seed, is dominant. The formal system is too small to contribute significantly to improve that situation. The informal seed system should prioritize...

  18. Identification of thickspike wheatgrass accessions with superior rhizome spread, seed production, and seed quality

    Thickspike wheatgrass is widely used for rangeland seeding and revegetation projects in western areas of North America. However, thickspike wheatgrass does not produce high seed yields, and seed is often of poor quality, which often results in high seed prices and poor establishment following seedi...

  19. Evaluation of antioxidant and antiradical properties of Pomegranate (Punica granatum L.) seed and defatted seed extracts.

    Basiri, Shadi

    2015-02-01

    Pomegranate seeds are byproducts of the Pomegranate juice industries that contains functional compounds such as phenols. This study was aimed to evaluate the effect of solvents on extraction from Pomegranate seed and Pomegranate defatted seed and to measure the yield extract and phenolic content and antioxidant properties. For this purpose, the seeds and defatted seeds were directly isolated from fruits and seeds by cold pressing respectively, then were crushed and extracted with different solvents, including water, Methanol, Acetone, Ethyl acetate and Hexane and finally the extracts of them were evaluted. Phenolic compounds, ferric reducing-antioxidant power and radicals scavenging property of extracts were measured. The results showed the highest extraction efficiencies were for Hexane and Acetone solvents in extraction of seed and defatted seed respectively. The highest phenolic content was obtained from Methanol seed extract. Reducing activity test proved that the Methanol extracts of Pomegranate seed and Pomegranate defatted seed had the highest reducing strength. Results of radical scavenging activity were similar to reducing activity results. The order of antioxidant capacity of Pomegranate seed and Pomegranate defatted seed were found to be Methanol > Water > Acetone > Butanol > Ethyl acetate > Hexane. It can be concluded Pomegranate seed, which possesses high levels of polyphenols, can be one of the sources of the natural antioxidants. The Methanol extract had a higher antioxidant efficiency than seed and defatted seed extracts. PMID:25694727

  20. Spinach seed quality - potential for combining seed size grading and chlorophyll flourescence sorting

    Deleuran, Lise Christina; Olesen, Merete Halkjær; Boelt, Birte

    2013-01-01

    Spinach (Spinacia oleracea L.) seed quality is important for subsequent establishment of baby leaf and other spinach products. The indeterminate flowering pattern in spinach produces seeds of different sizes, and consequently a non-uniform seed lot. Sorting seeds based on novel sorting methods mi...

  1. Wheat and barley seed systems in Ethiopia and Syria

    Bishaw, Z.

    2004-01-01

    Keywords: Wheat,Triticumspp., Barley,Hordeumvulgare L., Seed Systems, Formal Seed Sector, Informal Seed Sector, National Seed Program, Seed Source, Seed Selection, Seed Management, Seed Quality, Genetic Diversity, Ethiopia, SyriaInEthiopiaandSyria, wheat and barley are the two most important principal cereal crops grown since ancient times.Manygenerations of natural and human selection led into highly adapted and diverse populations of local landraces. For most of the history of agriculture, ...

  2. Imagining a Genetic Seed Bank

    Cassarino, Stacie

    2012-01-01

    If “magical thinking,” to use her words, is essential to Suzanne Anker’s practice of integrating science and visual art, it is also indispensable to the viewer entering her recent exhibition, Genetic Seed Bank, at the Art | Sci Gallery in the California Nanosystems Institute at UCLA.

  3. SEED BIOLOGY OF MEDICAGO TRUNCATULA

    The seed of Medicago truncatula have morphological features typical of dicotyledons, and are borne in a spine-covered spiral pod. The cotyledons are rich in protein (35-45%), the major storage molecule. They accumulate approximately 10% lipids and only traces of starch. The most abundant proteins a...

  4. Drying kinetics of jatropha seeds

    Valdiney Cambuy Siqueira

    2012-04-01

    Full Text Available Given the necessity of developing jatropha cultivation equipment, this work adjusted different mathematical models to experimental data obtained from the drying of jatropha seeds submitted to different drying conditions and selected the best model to describe the drying process. The experiment was carried out at the Federal Institute of Goiás - Rio Verde Campus. Seeds with initial moisture content of approximately 0.50 (kg water/kg dry matter were dried in a forced air-ventilated oven, at temperatures of 45, 60, 75, 90 and 105°C to moisture content of 0.10 ± 0.005 (kg water/kg dry matter. The experimental data were adjusted to 11 mathematical models to represent the drying process of agricultural products. The models were compared using the coefficient of determination, chi-square test, relative mean error, estimated mean error and residual distribution. It was found that the increase in the air temperature caused a reduction in the drying time of seeds. The models Midilli and Two Terms were suitable to represent the drying process of Jatropha seeds and between them the use of the Midili model is recommended due to its greater simplicity.

  5. Securing Access to Seed: Social Relations and Sorghum Seed Exchange in Eastern Ethiopia

    McGuire, S.

    2008-01-01

    Access to seed is crucial for farming, though few studies investigate household-level access in the informal `farmer seed systems¿ which still supply most seed in poor countries. This paper uses empirical data of seed exchange practices for sorghum in eastern Ethiopia to analyze how social relationships influence access to off-farm seed for a major crop. Seed shortfalls are common, and farmer¿farmer exchange is important for providing locally-adapted seed to fill this gap, but access varies c...

  6. Cryopreservation of orthodox and recalcitrant seed

    This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for cryopreservation of plant seeds in liquid nitrogen. It includes two exercises involving step by step protocols for use with the two major types of seeds. A standard technique for small seed...

  7. Staying Alive: Molecular Aspects of Seed Longevity.

    Sano, Naoto; Rajjou, Loïc; North, Helen M; Debeaujon, Isabelle; Marion-Poll, Annie; Seo, Mitsunori

    2016-04-01

    Mature seeds are an ultimate physiological status that enables plants to endure extreme conditions such as high and low temperature, freezing and desiccation. Seed longevity, the period over which seed remains viable, is an important trait not only for plant adaptation to changing environments, but also, for example, for agriculture and conservation of biodiversity. Reduction of seed longevity is often associated with oxidation of cellular macromolecules such as nucleic acids, proteins and lipids. Seeds possess two main strategies to combat these stressful conditions: protection and repair. The protective mechanism includes the formation of glassy cytoplasm to reduce cellular metabolic activities and the production of antioxidants that prevent accumulation of oxidized macromolecules during seed storage. The repair system removes damage accumulated in DNA, RNA and proteins upon seed imbibition through enzymes such as DNA glycosylase and methionine sulfoxide reductase. In addition to longevity, dormancy is also an important adaptive trait that contributes to seed lifespan. Studies in Arabidopsis have shown that the seed-specific transcription factor ABSCISIC ACID-INSENSITIVE3 (ABI3) plays a central role in ABA-mediated seed dormancy and longevity. Seed longevity largely relies on the viability of embryos. Nevertheless, characterization of mutants with altered seed coat structure and constituents has demonstrated that although the maternally derived cell layers surrounding the embryos are dead, they have a significant impact on longevity. PMID:26637538

  8. Hybrid seed production tests at Stuttgart, AR

    Hybrid seed production is complicated and difficult compared to seed increase of conventional inbred rice varieties. Some factors to be considered in hybrid seed production include whether it is a two or three-line hybrid, geographic location, field isolation, planting sequence for prental lines, fi...

  9. Mutation breeding in seed spices

    Seed spice is one of the important groups of crops cultivated in India for their large domestic consumption and bright export potential. But while the yield potential of the crops covered under this group is generally low, these crops generally also suffer from lack of usable variation for important yield traits and disease resistance in the germplasm collection and even if present may not be used with ease on account of very small size of their flower, thus restricting the crop improvement programs. We have, therefore, applied mutation breeding using gamma irradiation and chemical mutagens (EMS and Sodium Azide) for creation of variability for improvement of yield of major seed spice crops like Cumin, Coriander, Fennel and Fenugreek. Both M1 and M2 generations resulting from treatment of the mutagens were studied in respect of yield and yield attributes and other phenotypic alterations (Chlorophyll and other macro mutations) in certain genotype of these crops. Fenugreek was found relatively most radio and chemo-resistant followed by cumin. Mutagenic efficiency also varied noticeably between crops and mutagens; gamma rays were relatively more potent on cumin as compared to chemical mutagens whereas on fennel it was just reverse. Efficient mutagens more often yielded superior M2 progenies, i.e. progenies with significantly higher yield than their parent in fenugreek, fennel and cumin. Seed yield per plant of M2 progenies varied to different extents e.g. the yield was as high as 289% in coriander, 269 % in cumin, 122% in fennel and least in fenugreek (83%). In coriander specifically, one of the advance generation mutant showed increase in essential oil content along with seed yield comparable to the parent. The usefulness of induced mutations for improvement of seed yield is discussed (author)

  10. EFEITOS DE PRODUTOS QUÍMICOS NO CONTROLE DE Meloidogyne sp SOBRE QUIABEIRO (Hibiscus sculentum L. EFFECTS OF CHEMICAL CONTROL OF Meloidogyne sp. IN Hibiscus sculentum L. IN OKRA

    Lincoln Fonseca Zica

    2007-09-01

    Full Text Available

    Testou-se o poder nematicida de Shell-DD, Vapan, Nemagon granulado, brometo de metila e Neantina solúvel em solos com infestacão natural de Meloidogyne sp., usando-se o quiabeiro (Hibiscus escullentum L. como referência para medir a intensidade de infestação após o tratamento. O Shell-DD (40 ml/m² exerceu notável efeito nematicida, mas o Vapan (60 ml/m², o Nemagon granulado (40 g/m² e a Neantina solúvel (solução a 0,3% - 10 l/m² deram resultados estatisticamente iguais à testemunha. As parcelas tratadas com Brometo de metila (40 ml/m² foram perdidas em consequência do efeito fitotóxico do produto sobre as sementes e plântulas do quiabeiro.

    The nematicide power of Shell-DD, vapan, granulated nemagon, methyl bromide and soluble neantina, were tested by applying them to soil affected by Meloidogyne sp. The okra plant (Hibiscus esculentum L. was used as the hosting agent, to measure the amount of worms af-ter treatment. The results showed that Shell-DD (40 ml/m² controlled the worms well, but that vapan, granulated nemagon and the soluble neantina were statistically equal to the control plot. The plots treated with methyl bromide were not included in the results because of the toxic effect of the product on the plants.

  11. Caracterização físico-química de frutos de quatro cultivares de quiabo Physical and chemical characteristics from fruits of four okra cultivars

    Wagner F. da Mota

    2005-07-01

    Full Text Available Efetuou-se a caracterização físico-química dos frutos de quatro cultivares de quiabo neste estudo. O delineamento utilizado foi de blocos casualizados, sendo os tratamentos constituídos pelas cultivares Amarelinho, Red Velvet, Star of David e Mammoth Spinless, com cinco repetições. Na maturidade comercial os frutos da cultivar Star of David tiveram maior diâmetro, peso fresco total e teor de matéria seca, menor comprimento, teor de umidade e de vitamina C comparado com as demais cultivares. A cultivar Red Velvet teve o menor diâmetro, peso fresco, teor de matéria seca, açúcares redutores e teores de clorofilas a, b e total, e maior teor de umidade e vitamina C. Frutos da cultivar Mammoth Spinless apresentaram os maiores teores de clorofilas a, b e total. A cultivar Amarelinho teve maior comprimento e menores teores de clorofila, a, b e total. As cultivares Amarelinho e Mammoth Spinless apresentaram maiores teores de açúcares redutores.In an experiment some physical and chemical characteristics of four okra cultivars were evaluated. The experimental design was of randomized blocks, where the treatments were the cultivars Amarelinho, Red Velvet, Star of David and Mammoth Spinless with five replications. At commercial maturity fruits of the cultivar Star of David presented the largest diameter, total fresh matter, content of dry matter, smallest length, content of water and vitamin C in comparison to the other cultivars. The cultivar Red Velvet presented the smallest diameter, total fresh matter, content of dry matter, reducing sugars and content of chlorophyll a, b and total, but the largest content of water and vitamin C. The cultivar Amarelinho produced the longest fruits and the smallest content of chlorophyll a, b and total. The cultivars Amarelinho and Mammoth Spinless showed higher contents of total reducing sugars.

  12. Seed Systems Support in Kenya: Consideration for an Integrated Seed Sector Development Approach

    Peter Munyi; Bram De Jonge

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector Development (ISSD) approach. The ISSD approach has evolved as a response to the almost exclusive focus on formal seed systems in seed sector development programs. Instead, ISSD aims to recognize and sup...

  13. Seed disperser effectiveness: the quantity component and patterns of seed rain for Prunus mahaleb

    Jordano, Pedro; Schupp, Eugene W.

    2000-01-01

    In this paper we analyze the seed dispersal stage of the Prunus mahaleb–frugivorous bird inter- action from fruit removal through seed deliver y within the context of disperser effectiveness. The effectiveness of a frugivorous species as a seed disperser is the contribution it makes to plant fitness. Effectiveness depends on the quantity of seed dispersed (‘‘quantity component’’) and the quality of dispersal provided each seed (‘‘quality component’’). For the main frug...

  14. Gastropod Seed Dispersal: An Invasive Slug Destroys Far More Seeds in Its Gut than Native Gastropods

    Blattmann, Tamara; Boch, Steffen; Türke, Manfred; Knop, Eva

    2013-01-01

    Seed dispersal is one of the most important mechanisms shaping biodiversity, and animals are one of the key dispersal vectors. Animal seed dispersal can directly or indirectly be altered by invasive organisms through the establishment of new or the disruption of existing seed dispersal interactions. So far it is known for a few gastropod species that they ingest and defecate viable plant seeds and consequently act as seed dispersers, referred to as gastropodochory. In a multi-species experime...

  15. Water Productivity of Irrigated Rice under Transplanting, Wet Seeding and Dry Seeding Methods of Cultivation

    Murali, NS.; Thabonithy, R.

    1997-01-01

    Water productivity (WP) of irrigated lowland rice was determined during the 1994 dry (January to May) and wet (August to December) seasons on a heavy clay acid sulphate soil. Treatments consisted of three cultivation methods : transplanted rice, pregerminated seeds broadcasted on puddled soil (wet seeding) and dry seeds broadcasted on unpuddled soil (dry seeding). In wet and dry seeded plots, continuous standing water condition was initiated 17 days after sowing. Total water requirement for r...

  16. Seed germination, Dispersal and Seed Bank in Heracleum mantegazzianum

    Moravcová, Lenka; Pyšek, Petr; Krinke, L.; Pergl, Jan; Perglová, Irena; Thompson, K.

    Wallingford : CAB International, 2007 - (Pyšek, P.; Cock, M.; Nentwig, W.; Ravn, H.), s. 74-91 ISBN 978-1-84593-206-0 R&D Projects: GA ČR(CZ) GA206/05/0323 Grant ostatní: -(XE) EVK2-CT-2001-00128 Institutional research plan: CEZ:AV0Z60050516 Keywords : Germination * Dormancy * Seed Bank Subject RIV: EF - Botanics

  17. Comparative Analysis of Functional and Nutritive Values of Amla (Emblica officinalis Fruit, Seed and Seed Coat Powder

    Poonam Mishra

    2014-01-01

    Full Text Available In present investigation amla seed and seed coat was investigated separately for their compositional, functional property. Water retention capacity and swelling capacity of seed coat powder was higher than seed powder. Among the three, fruit powder showed strongest free radical (DPPH activity followed by seed coat whereas, seed had only 30% of DPPH* scavenging activity. Both seed and seed coat powder were found to be good sources of P, K, Mg, Fe etc. Seed powder had high value of P(395.44 mg/100 g in comparison to fruit powder and seed coat powder while seed coat was found to be a good source of Ca, Cr and Mn. The Total Phenolic Content (TPC of seed coat and seed powder was lower than fruit powder. The 9, 12, 15, octadecatrienoic acid (z, z, z and tetradecanoic acid were the major fatty acids present in seed and seed coat.

  18. 7 CFR 301.52 - Quarantine; restriction on interstate movement of specified regulated articles.

    2010-01-01

    ... of these plants, except: (i) Canned or frozen okra; or (ii) Okra seed; and (iii) Fresh, edible fruits... Register citations affecting § 301.52, see the List of CFR Sections Affected, which appears in the Finding... articles: (1) Cotton and wild cotton, including all parts of these plants. (2) Seed cotton. (3)...

  19. Endozoochorous seed dispersal by Japanese macaques (Macaca fuscata): Effects of temporal variation in ranging and seed characteristics on seed shadows.

    Tsuji, Yamato; Morimoto, Mayumi

    2016-02-01

    Variation in seed shadows generated by frugivores is caused by daily, seasonal, and inter-annual variation in ranging, as well as inter-specific variability in gut passage times according to seed characteristics. We studied the extent to which seed weight, specific gravity, and daily (morning, afternoon, and evening) and inter-annual (2004 vs. 2005) variation in ranging affected seed shadows generated by wild Japanese macaques (Macaca fuscata) in northern Japan. The macaques ingested fleshy fruits of 11 species during the two year study period; Viburnum dilatatum (Caprifoliaceae: heavier seeds with higher specific gravity) and Rosa multiflora (Rosaceae: lighter seeds with lower specific gravity) were eaten frequently in both years. The travel distances of macaques after feeding on V. dilatatum and R. multiflora fruits were estimated by combining feeding locations and ranging patterns measured in the field with gut passage times of model seeds in captive animals. Median travel distances after fruit feeding were 431 (quantile range: 277-654) and 478 m (265-646), respectively, with a maximum of 1,261 m. Neither year nor time of day affected travel distances. The gut passage time of model V. dilatatum seeds was longer than that of model R. multiflora seed, but this did not affect dispersal distances. Seed shadows for both species over 2 years showed unimodal distribution (peak: 101-500 m) and more than 90%, 20%, and 3% of ingested seeds were estimated to be dispersed >100, >500, and >1000 m, respectively, the longest known distances among macaque species. R. multiflora seeds tended to be dispersed further in 2004 than 2005, but V. dilatatum seeds were not, implying that inter-annual variations in ranging pattern due to the distribution and abundance of nut fruiting could affect dispersal distance. Am. J. Primatol. 78:185-191, 2016. © 2015 Wiley Periodicals, Inc. PMID:26469699

  20. Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

    Seyed Ata SIADAT

    2015-09-01

    Full Text Available Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm and different duration of treatment (8, 12 and 24 hour. Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

  1. [Genomic imprinting and seed development].

    Zhang, Wen-Wei; Cao, Shao-Xian; Jiang, Ling; Zhu, Su-Song; Wan, Jian-Min

    2005-07-01

    The endosperm, a seed tissue that mediates the transfer of nutrients from the maternal parent to the embryo, is an important site of imprinting in flowering plants. In Arabidopsis thaliana, three genes were identified that prevent fertilization-independent seed development: FIS1/MEDEA, FIS2 and FIS3/FIE. MEDEA (MEA), a master regulator of endosperm development, is known to be imprinted in the endosperm. FWA is also imprinted in the endosperm of the model plant Arabidopsis. The following aspects were included in the present review: the imprinting mechanism in angiosperms, the latest progress in the control of MEA and FWA imprinting, the parental conflict theory to explain imprinting, the imprinting methods and other imprinted genes found in plants. PMID:16120596

  2. EFFICIENCY OF POMEGRANATE SEED OIL

    Sukru Karatas; Nihan Ozdogan

    2014-01-01

    In this paper pomegranate kinds that reach to harvest maturity which are produced in commercial scales in Adana, Antalya, Finike and Kilis regions in Turkey and also Hicaz and Eksilik kinds are chosen randomly to be investigated. Seed efficiencies, grain ratios, oil efficiency of these chosen pomegranate kinds were studied. According to this study, pomegranate kind taken from Kilis region is found to have the highest grain efficieny with a ratio of 71% and pomegranate kind taken from...

  3. Apiaceae seeds as functional food

    Aćimović Milica G.; Kostadinović Ljiljana M.; Popović Sanja J.; Dojčinović Nevena S.

    2015-01-01

    The aim of this review was to point to a great importance of plants from Apiaceae family as a functional food. Caraway (Carum carvi L.), anise (Pimpinella anisum L.), coriander (Coriandrum sativum L.), dill (Anethum graveolens L.), fennel (Foeniculum vulgare Mill.) and cumin (Cuminum cyminum L.) are plants from the above-mentioned family whose seeds are widely used in folk medicine, pharmaceutical industry, as spices, flavoring agents and as dietary supplem...

  4. Peroxidase: Purification from Soybean Seeds

    Farzana Habib

    2003-01-01

    Full Text Available Peroxidase, extracted from soybean seeds and was partially purified by precipitating with ammonium sulfate and 85 % saturation resulted in an increase in the activity of peroxidase up to 1.21 fold. Purification was carried out by diethyl amminoethyl cellulose chromatography and purification fold obtained was 2.62. Then, the enzyme was subjected to 10 % sodium dodecyl sulfate polyacrylamide gel electrophoresis which resulted in a marked decrease in unwanted proteins.

  5. Sunflower Seed and Acne Vulgaris

    Mohebbipour, Alireza; Sadeghi-Bazargani, Homayoun; Mansouri, Mona

    2015-01-01

    Background: Regardless of the overall association between diet and acne which cannot be easily ignored, there might be an association between specific nutrients and acne development or improvement. Objectives: The aim of this study was to assess the effect of dietary intake of sunflower seeds on acne severity and the pattern of acne lesions. Patients and Methods: In a randomized controlled trial, 50 patients aged 15 - 30 years old with acne vulgaris were enrolled through consecutive convenient sampling, in a dermatology clinic in Ardabil, Iran. They were randomly allocated into two trial arms. Those in the control group were asked to stop eating sunflower seeds if they did before. In the intervention group, they consumed 25 g sunflower-containing food daily for seven days. The primary outcome of interest was 10% increase/decrease in the baseline acne severity index (ASI), sustained to the end of the follow-up period on day 14. Results: The mean ASI did not change significantly through the study period in the control group, but it increased in the sunflower group from 62 at the baseline to 86.8 after two weeks (P acne grading score (GAGS) did not significantly change in any of the groups and the difference in the change of GAGS was not significant between the groups (2.4 in the sunflower group versus 1.6 in the control group). Twenty two subjects (88%) in the sunflower group versus 9 (36%) in the control group had at least 10% increment in ASI throughout the follow-up period (P acne vulgaris; however, further evidence is needed to ban sunflower seed intake in patients with acne. Considering the observed potential negative effect in this trial, future randomized clinical trials may base their design on randomly assigning the exposed patients to give up use of sunflower seed intake. PMID:26473070

  6. Hormones and tomato seed germination.

    Liu, Y.(Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui, China)

    1996-01-01

    Using GA- and ABA-deficient mutants, exogenous gibberellins (GAs), abscisic acid (ABA) and osmoticum, we studied the roles of GAs and ABA in the induction of cell cycle activities, internal free space formation and changes in water relations during seed development and imbibition in tomato. First of all, it was demonstrated that endogenous ABA plays an important role in induction of primary dormancy, prevention of viviparous germination and arresting cell cycle activities of the radicle-tip c...

  7. Cultivating knowledge on seed systems and seed strategies: Case of the rice crop

    Amadou Moustapha Bèye

    2014-01-01

    Full Text Available This review gives key information about seed systems with the objective of helping countries in sub-Saharan Africa (SSA to design appropriate strategies based on their own local realities. It starts with an in-depth assessment of the rice seed sector and emphasizes the factors that can influence its development. These factors may be associated with the biological characteristics of the rice crop, the complexity of farming systems, the policy environment, and the markets. The historical background of the seed sector in SSA is described in order to give a clear picture of the different attempts to find solutions in different countries and sub-regions. Five major strategic models and several alternative seed strategies are identified. However, local traditional seed systems will remain the primary source of seed supply for the majority of farmers for many years to come. These systems are based on farmer-saved seed and farmer-to-farmer seed exchanges, which are efficient tools in seed dissemination and food security. It is time to recognize local traditional systems and their contribution to the maintenance of seed quality and crop diversity and to develop tools for their better utilization. The community-based seed system (CBSS is proposed as such a tool. CBSS encourages technicians to recognize local traditional seed systems and to build integrated seed approaches that take into consideration local realities and the socio-cultural dimension of seed within African communities. This will likely require a transition period of 5 to 10 years to build the fundamentals of sustainable seed systems through the structuring of the seed sector and the creation of appropriate conditions to ensure food security, enriched biodiversity and sustainable production. With climate change, local traditional seed systems, as well as integrated seed approaches, will likely play a more important role to improve the performance of agricultural systems while ensuring farmer autonomy.

  8. Ballistic seed projection in two herbaceous species.

    Garrison, W J; Miller, G L; Raspet, R

    2000-09-01

    We found that the majority of ballistic seeds tested [N = 74 of 78 in Vicia sativa ssp. nigra (Fabaceae); N = 25 of 27 in Croton capitatus var. capitatus (Euphorbiaceae)] were projected at angles that would yield a greater distance than the average of seeds with the same initial speed projected at random angles. In addition, the median of fractional distance error (maximum distance - seed distance)/(maximum distance), of the seeds were 0.11 and 0.04 for V. sativa and C. capitatus, respectively. Seed projection distance was modeled by using initial projection angle, initial speed, and measured drag, along with other seed data. We improved upon previous such studies by using dual-angle high-speed stroboscopic photography to determine initial projection angle and speed. We also measured seed drag in a low-turbulence wind tunnel. Seed projection positions on the plant, which also affect seed projection distance, were found to be primarily from the top of the plant, with 98 of 137 and 407 of 407 fruits in the upper half of the plant for V. sativa and C. capitatus, respectively. Our findings are significant because they suggest that in addition to the ballistic projection mechanism itself, the species studied have additional adaptations that result in enhanced seed projection distance from the parent plant. PMID:10991896

  9. Local evolution of seed flotation in Arabidopsis.

    Saez-Aguayo, Susana; Rondeau-Mouro, Corinne; Macquet, Audrey; Kronholm, Ilkka; Ralet, Marie-Christine; Berger, Adeline; Sallé, Christine; Poulain, Damien; Granier, Fabienne; Botran, Lucy; Loudet, Olivier; de Meaux, Juliette; Marion-Poll, Annie; North, Helen M

    2014-03-01

    Arabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilage release and float. Four of these have a novel mucilage phenotype with almost none of the released mucilage adhering to the seed and the absence of cellulose microfibrils. Mucilage release was modified in the variants by ten independent causal mutations in four different loci. Seven distinct mutations affected one locus, coding the MUM2 β-D-galactosidase, and represent a striking example of allelic heterogeneity. The modification of mucilage release has thus evolved a number of times independently in two restricted geographical zones. All the natural mutants identified still accumulated mucilage polysaccharides in seed coat epidermal cells. Using nuclear magnetic resonance (NMR) relaxometry their production and retention was shown to reduce water mobility into internal seed tissues during imbibition, which would help to maintain seed buoyancy. Surprisingly, despite released mucilage being an excellent hydrogel it did not increase the rate of water uptake by internal seed tissues and is more likely to play a role in retaining water around the seed. PMID:24625826

  10. Galactinol as marker for seed longevity.

    de Souza Vidigal, Deborah; Willems, Leo; van Arkel, Jeroen; Dekkers, Bas J W; Hilhorst, Henk W M; Bentsink, Leónie

    2016-05-01

    Reduced seed longevity or storability is a major problem in seed storage and contributes to increased costs in crop production. Here we investigated whether seed galactinol contents could be predictive for seed storability behavior in Arabidopsis, cabbage and tomato. The analyses revealed a positive correlation between galactinol content and seed longevity in the three species tested, which indicates that this correlation is conserved in the Brassicaceae and beyond. Quantitative trait loci (QTL) mapping in tomato revealed a co-locating QTL for galactinol content and seed longevity on chromosome 2. A candidate for this QTL is the GALACTINOL SYNTHASE gene (Solyc02g084980.2.1) that is located in the QTL interval. GALACTINOL SYNTHASE is a key enzyme of the raffinose family oligosaccharide (RFO) pathway. To investigate the role of enzymes in the RFO pathway in more detail, we applied a reverse genetics approach using T-DNA knock-out lines in genes encoding enzymes of this pathway (GALACTINOL SYNTHASE 1, GALACTINOL SYNTHASE 2, RAFFINOSE SYNTHASE, STACHYOSE SYNTHASE and ALPHA-GALACTOSIDASE) and overexpressors of the cucumber GALACTINOL SYNTHASE 2 gene in Arabidopsis. The galactinol synthase 2 mutant and the galactinol synthase 1 galactinol synthase 2 double mutant contained the lowest seed galactinol content which coincided with lower seed longevity. These results show that galactinol content of mature dry seed can be used as a biomarker for seed longevity in Brassicaceae and tomato. PMID:26993241

  11. Electrophysiology of pumpkin seeds: Memristors in vivo.

    Volkov, Alexander G; Nyasani, Eunice K; Tuckett, Clayton; Greeman, Esther A; Markin, Vladislav S

    2016-04-01

    Leon Chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. We recently found memristors in different plants such as the Venus flytrap, Mimosa pudica, Aloe vera, apple fruits, and in potato tubers. There are no publications in literature about the existence of memristors in seeds. The goal of this work was to discover if pumpkin seeds might have memristors. We selected Cucurbita pepo L., cv. Cinderella, Cucurbita maxima L. cv Warty Goblin, and Cucurbita maxima L., cv. Jarrahdale seeds for this analysis. In these seeds, we found the presence of resistors with memory. The analysis was based on cyclic voltammetry where a memristor should manifest itself as a nonlinear two-terminal electrical element, which exhibits a pinched hysteresis loop on a current-voltage plane for any bipolar cyclic voltage input signal. Dry dormant pumpkin seeds have very high electrical resistance without memristive properties. The electrostimulation by bipolar sinusoidal or triangular periodic waves induces electrical responses in imbibed pumpkin seeds with fingerprints of memristors. Tetraethylammonium chloride, an inhibitor of voltage gated K(+) channels, transforms a memristor to a resistor in pumpkin seeds. NPPB (5-Nitro-2-(3-phenylpropylamino)benzoic acid) inhibits the memristive properties of imbibed pumpkin seeds. The discovery of memristors in pumpkin seeds creates a new direction in the understanding of electrophysiological phenomena in seeds. PMID:26926652

  12. Physicochemical Properties of Moringa stenopetala (Haleko Seeds

    Eyassu Seifu

    2012-01-01

    Full Text Available The physical and chemical properties of Moringa stenopetala (Haleko seeds produced in Mella district of Gamo Gofa Zone in southern Ethiopia are reported here for the first time. The average weight of the whole intact seeds (g/100 seeds, the kernel (g/100 seeds and an individual seed (g seed-1 was 73.6, 59.6 and 0.6 g, respectively. The kernel accounted for 79.7% of the seed’s dry weight and the hull accounted for 20.3%. The M. stenopetala seeds analyzed had an average length, width and bulk density of 17.6 mm, 8.2 mm and 0.9 g cm3, respectively. The mean moisture, oil, protein, ash and crude fiber contents of the M. stenopetala seeds analyzed were 6.1, 41.4, 42.6, 4.6 and 5.1 (g/100 g, respectively. The residue that remained after oil extraction of the seeds had an average crude protein content of 55.6 (g/100 g. The results showed that M. stenopetala seed contains high oil and protein contents suggesting that it could be used as important source of oil and protein.

  13. Radioactive seed immobilization techniques for interstitial brachytherapy

    Yan, K.; Podder, T.; Buzurovic, I.; Hu, Y.; Dicker, A.; Valicenti, R.; Yu, Y. [Thomas Jefferson University, Department of Radiation Oncology, Philadelphia, PA (United States); Messing, E. [University of Rochester, Departments of Urology and Surgery, Rochester, NY (United States); Rubens, D. [University of Rochester, Departments of Imaging Science and Surgery, Rochester, NY (United States); Sarkar, N. [Vanderbilt University, Department of Mechanical Engineering, Nashville, TN (United States); Ng, W. [Nangyang Technical University, School of Mechanical and Aerospace Engineering, Singapore (Singapore)

    2008-06-15

    In prostate brachytherapy, seeds can detach from their deposited sites and move locally in the pelvis or migrate to distant sites including the pulmonary and cardiac regions. Undesirable consequences of seed migration include inadequate dose coverage of the prostate and tissue irradiation effects at the site of migration. Thus, it is clinically important to develop seed immobilization techniques. We first analyze the possible causes for seed movement, and propose three potential techniques for seed immobilization: (1) surgical glue, (2) laser coagulation and (3) diathermy coagulation. The feasibility of each method is explored. Experiments were carried out using fresh bovine livers to investigate the efficacy of seed immobilization using surgical glue. Results have shown that the surgical glue can effectively immobilize the seeds. Evaluation of the radiation dose distribution revealed that the non-immobilized seed movement would change the planned isodose distribution considerably; while by using surgical glue method to immobilize the seeds, the changes were negligible. Prostate brachytherapy seed immobilization is necessary and three alternative mechanisms are promising for addressing this issue. Experiments for exploring the efficacy of the other two proposed methods are ongoing. Devices compatible with the brachytherapy procedure will be designed in future. (orig.)

  14. Suppressive mechanism of seed coat pigmentation in yellow soybean

    Senda, Mineo; Kurauchi, Tasuku; Kasai, Atsushi; Ohnishi, Shizen

    2012-01-01

    In soybean seeds, numerous variations in colors and pigmentation patterns exist, most of which are observed in the seed coat. Patterns of seed coat pigmentation are determined by four alleles (I, ii, ik and i) of the classically defined I locus, which controls the spatial distribution of anthocyanins and proanthocyanidins in the seed coat. Most commercial soybean cultivars produce yellow seeds with yellow cotyledons and nonpigmented seed coats, which are important traits of high-quality seeds...

  15. Selected aspects of tiny vetch [Vicia hirsuta (L. Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination

    Magdalena Kucewicz

    2012-12-01

    Full Text Available Vicia hirsuta (L. Gray S.F. (tiny vetch is a common and persistent segetal weed. Tiny vetch seeds and pods reach different stages of maturity during the crop harvest season. Some seeds that mature before cereal harvest are shed in the field and deposited in the soil seed bank, while others become incorporated into seed material. The objective of this study was to describe selected aspects of tiny vetch seed ecology: to determine the rate of individual reproduction of vetch plants growing in winter and spring grain crops and to evaluate the germination of seeds at different stages of maturity, subject to storage conditions. The seeds and pods of V. hirsuta were sorted according to their development stages at harvest and divided into two groups. The first group was stored under laboratory conditions for two months. In the autumn of the same year, the seeds were subjected to germination tests. The remaining seeds were stored in a storeroom, and were planted in soil in the spring. The germination rate was evaluated after 8 months of storage. Potential productivity (developed pods and flowers, fruit buds was higher in plants fruiting in winter wheat than in spring barley. Vetch plants produced around 17-26% more pods (including cracked, mature, greenish-brown and green pods and around 25% less buds in winter wheat than in spring barley. Immature seeds were characterized by the highest germination capacity. Following storage under laboratory conditions and stratification in soil, mature seeds germinated at a rate of several percent. After storage in a storeroom, seeds at all three development stages broke dormancy at a rate of 72- 75%. The high germination power of tiny vetch seeds stored in a storeroom indicates that this plant can be classified as an obligatory speirochoric weed species.

  16. Seed source, seed traits, and frugivore habits: Implications for dispersal quality of two sympatric primates.

    Benítez-Malvido, Julieta; González-Di Pierro, Ana Ma; Lombera, Rafael; Guillén, Susana; Estrada, Alejandro

    2014-06-11

    • Premise of the study: Frugivore selection of fruits and treatment of seeds together with seed deposition site are crucial for the population dynamics of vertebrate-dispersed plants. However, frugivore species may influence dispersal quality differently even when feeding on the same fruit species and, while animals disperse some seeds, others simply fall beneath the parent plant.• Methods: In southern Mexico, we investigated to see if within-species seed traits (i.e., length, width, weight, and volume) and germination success differed according to seed source. For five tropical tree species we obtained ingested seeds from two sources, howler monkey (Alouatta pigra) and spider monkey (Ateles geoffroyi) feces; and noningested seeds from two sources, the ground and tree crowns (with predispersed seeds used as control).• Key results: A principal components' analysis showed that traits of seeds ingested by howler monkeys differed from other sources while seeds ingested by spider monkeys were similar to noningested seeds. Howlers consumed on average the larger seeds in Ampelocera hottlei, Brosimum lactescens, and Dialium guianense. Both primate species consumed the smaller seeds in Spondias mombin, while no seed trait differences among seed sources were found in Spondias radlkoferi. For all five tree species, germination rate was greatest for seeds ingested by howler monkeys.• Conclusions: For the studied plant species, seed ingestion by howler monkeys confers higher dispersal quality than ingestion by spider monkeys or nondispersal. Dispersal services of both primate species, however, are not redundant and may contribute to germination heterogeneity within plant populations in tropical forests. PMID:24920763

  17. Effects of rodent species, seed species, and predator cues on seed fate

    Sivy, Kelly J.; Ostoja, Steven M.; Schupp, Eugene W.; Durham, Susan

    2011-07-01

    Seed selection, removal and subsequent management by granivorous animals is thought to be a complex interaction of factors including qualities of the seeds themselves (e.g., seed size, nutritional quality) and features of the local habitat (e.g. perceived predator risk). At the same time, differential seed selection and dispersal is thought to have profound effects on seed fate and potentially vegetation dynamics. In a feeding arena, we tested whether rodent species, seed species, and indirect and direct predation cues influence seed selection and handling behaviors (e.g., scatter hoarding versus larder hoarding) of two heteromyid rodents, Ord's kangaroo rat ( Dipodomys ordii) and the Great Basin pocket mouse ( Perognathus parvus). The indirect cue was shrub cover, a feature of the environment. Direct cues, presented individually, were (1) control, (2) coyote ( Canis latrans) vocalization, (3) coyote scent, (4) red fox ( Vulpes vulpes) scent, or (5) short-eared owl ( Asio flammeus) vocalization. We offered seeds of three sizes: two native grasses, Indian ricegrass ( Achnatherum hymenoides) and bluebunch wheatgrass ( Pseudoroegneria spicata), and the non-native cereal rye ( Secale cereale), each in separate trays. Kangaroo rats preferentially harvested Indian ricegrass while pocket mice predominately harvested Indian ricegrass and cereal rye. Pocket mice were more likely to scatter hoard preferred seeds, whereas kangaroo rats mostly consumed and/or larder hoarded preferred seeds. No predator cue significantly affected seed preferences. However, both species altered seed handling behavior in response to direct predation cues by leaving more seeds available in the seed pool, though they responded to different predator cues. If these results translate to natural dynamics on the landscape, the two rodents are expected to have different impacts on seed survival and plant recruitment via their different seed selection and seed handling behaviors.

  18. [Seed rain and seed bank of constructive species in evergreen broadleaved forest at Chongqing Simian Mountain].

    Peng, J; Li, X; Fu, Y; Liu, Y

    2000-02-01

    The study shows that the early and late seed rains of constructive tree species in evergreen broadleaved forest at Chongqing Simian Mountain had no activity. The bigger the seeds of the species and the earlier or later the seeds of the species matured, the higher the proportion of the seed rain damaged by animals. The quantitative variation of seed rain, active seed rain and seed bank did not take place at the same time. At early time, the number of seed banks of Castanopsis fargesii, Lithocarpus glabra, Quercus myrsinrefolia, Castanopsis plasyacantha, Cinamomum subavenium which own more active seed rain increased by geomitric series. The seed banks of Castanopsis orthacantha and Schima argentea were small, only survived a short time, and did not sprout next year. The quantitative dynamics of seed banks and their increasing or decreasing rates were decided by the proportion damaged by animals, dying speed of seeds and their resistance to pathogens and adverse circumstances, and the state of seed germination. PMID:11766580

  19. Tannin concentration enhances seed caching by scatter-hoarding rodents: An experiment using artificial ‘seeds’

    Wang, Bo; Chen, Jin

    2008-11-01

    Tannins are very common among plant seeds but their effects on the fate of seeds, for example, via mediation of the feeding preferences of scatter-hoarding rodents, are poorly understood. In this study, we created a series of artificial 'seeds' that only differed in tannin concentration and the type of tannin, and placed them in a pine forest in the Shangri-La Alpine Botanical Garden, Yunnan Province of China. Two rodent species ( Apodemus latronum and A. chevrieri) showed significant preferences for 'seeds' with different tannin concentrations. A significantly higher proportion of seeds with low tannin concentration were consumed in situ compared with seeds with a higher tannin concentration. Meanwhile, the tannin concentration was significantly positively correlated with the proportion of seeds cached. The different types of tannin (hydrolysable tannin vs condensed tannin) did not differ significantly in their effect on the proportion of seeds eaten in situ vs seeds cached. Tannin concentrations had no significant effect on the distance that cached seeds were carried, which suggests that rodents may respond to different seed traits in deciding whether or not to cache seeds and how far they will transport seeds.

  20. Reasoned opinion on the modification of the existing MRLs for bixafen in rape seed, linseed, poppy seed and mustard seed

    European Food Safety Authority

    2013-01-01

    In accordance with Article 6 of Regulation (EC) No 396/2005, the United Kingdom, hereafter referred to as the evaluating Member State (EMS), received an application from Bayer CropSciences AG to modify the existing MRL for the active substance bixafen in rape seed. The submitted residue data support the intended use of bixafen in rape seed, linseed, poppy seed and mustard seed in France as well. In order to accommodate for the intended use of bixafen, the United Kingdom proposed to raise the ...

  1. Reasoned opinion on the modification of the existing MRLs for prothioconazole in rape seed, linseed, poppy seed and mustard seed

    European Food Safety Authority

    2012-01-01

    In accordance with Article 6 of Regulation (EC) No 396/2005, France, hereafter referred to as the evaluating Member State (EMS), received an application from Bayer CropScience to modify the existing MRLs for the active substance prothioconazole in rape seed, linseed, poppy seed and mustard seed. In order to accommodate the intended uses of prothioconazole, France proposed to raise the existing MRLs from the value of 0.1 mg/kg for rape seed and 0.05 mg/kg for the other three oilseeds to 0.15 m...

  2. Water uptake curve in physic nut seeds

    Alex Caetano Pimenta

    2014-09-01

    Full Text Available Physic nut (Jatropha curcas L. is a useful species in oil production for energy purposes, however its agricultural crop counts on little scientific knowledge. This work aimed to determine the water uptake curve in physic nut seeds, with the purpose to help future researches depending on this information. Two batches of seeds were used, represented by two sub-samples (intact seeds and dead seeds of each batch. The water uptake curve was determined weighing samples at specified intervals of time. A fully randomized experimental delineation was applied, with two treatments and four repetitions of 10 seeds each. Data were submitted to Bartlett, Anova and Turkey tests; regression equations were adjusted for the uptake curves. It was concluded that Jatropha curcas seeds follow the three-phases water uptake pattern , being phase I between 0 and 15h, phase II between 15h and 60h and phase three starting after 60h.

  3. Arborescent palm seed morphology and seedling distribution.

    Salm, Rodolfo

    2005-11-01

    This study examines how the seed morphology of two large arborescent palms, Attalea maripa (Aubl.) Mart. and Astrocaryum aculeatum G. Mey, may affect their seed shadow in a seasonally dry Amazonian forest. In addition to being smaller and produced in larger numbers than those of A. aculeatum, A. maripa seeds also presented a substantially lower amount of nutritional reserves available for the embryo. However, A. maripa seedlings were found in much higher numbers than those of A. aculeatum. The results suggest that, within the spatial scale considered, the seed rain of A. maripa is more restricted to the area surrounding around reproductive conspecifics than that of A. aculeatum. Furthermore, in comparison with those of A. aculeatum, the smaller seeds of A. maripa might be less attractive to scatterhoarding rodents (e.g. Dasyprocta aguti). The pattern observed emphasizes the importance of scatterhoarding rodents as dispersers of large-seeded plant species in Neotropical forests. PMID:16532195

  4. Irradiation effect and preservation of Ginkgo seeds

    Ginkgo seeds were treated with various doses of 60Co γ-ray. When treated with 50 Gy, Ginkgo seeds could not sprout because of dealdembryos. But the endosperm was still vigorous in a relative long period when treated with 800 Gy and over 2 kGy, causing Ginkgo seeds to lose water more quickly, show higher conductivity and the endosperm softened and die. The respiration of Ginkgo seeds was restrained greatly after γ-irradiation. When Ginkgo seeds were treated with 50∼200 Gy γ irradiation and preserved in normal atmospheric temperature after air tight wrapping with plastic film, the preservation period of the seeds was 2 to 3 months with water content of 40.25% and 4 to 6 months water content of 35.96%, respectively

  5. Dehydration of Seeds in Intact Tomato Fruits.

    McIlrath, W J; Abrol, Y P; Heiligman, F

    1963-12-27

    Although the moisture content of the placenta and pericarp of tomato fruits older than 15 days never dropped below about 94 percent, the seeds dehydrated to approximately 50 percent moisture as these organs developed and matured. There was a loss in the total amount, as well as the percentage, of water per seed. Part of this dehydration was mediated by the seeds themselves and part appeared to be due to other portions of the fruit. PMID:17834377

  6. 7 CFR 201.16 - Noxious-weed seeds.

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Noxious-weed seeds. 201.16 Section 201.16 Agriculture... REGULATIONS Labeling Agricultural Seeds § 201.16 Noxious-weed seeds. (a) Except for those kinds of noxious-weed seeds shown in paragraph (b) of this section, the names of the kinds of noxious-weed seeds and...

  7. The seed factor in forest establishment

    Oboho E. G.

    2016-05-01

    Full Text Available Large scale deforestation all over the world necessitates planting of more trees to augment the forest estate. Tree planting and forest production require the use of lots of seeds to raise seedlings. Factors of importance are those directly relating to the seed as well as those indirect factors affecting the germination of seeds, early growth and survival of seedlings. The main factors include physiological and physical attributes of seeds, factors controlling availability in time and quantity, seed extraction/handling, viability, storage, germination and early growth characteristics; nursery site condition, management know-how and dedicated staff are also vital to the success of forest production by affecting the sustenance of growth, development and survival of seedlings raised. Nursery staff, seed vendors and seedling producers should be properly trained in seed technology and nursery practices in order to enhance the quality and quantity of seedlings produced from available seeds. This is because seed is an invaluable regenerative propagule without which there would be neither seedlings nor forest establishment.

  8. Phase sensitive Raman process with correlated seeds

    A phase sensitive Raman scattering was experimentally demonstrated by injecting a Stokes light seed into an atomic ensemble, whose internal state is set in such a way that it is coherent with the input Stokes seed. Such phase sensitive characteristic is a result of interference effect due to the phase correlation between the injected Stokes light field and the internal state of the atomic ensemble in the Raman process. Furthermore, the constructive interference leads to a Raman efficiency larger than other kinds of Raman processes such as stimulated Raman process with Stokes seed injection alone or uncorrelated light-atom seeding. It may find applications in precision spectroscopy, quantum optics, and precise measurement

  9. PRINCIPLES AND PRACTICES OF SEED SELECTION NOWADAYS

    Efremova V. V.

    2015-02-01

    Full Text Available The article presents the author’s insight into the theoretical foundations of plant breeding for self-pollinators and cross-pollinators. From the author’s point of view, plant breeding techniques depend on a type of pollination, population propagation and isolation from other variety pollen. Adaptive properties of crops are described as ones depending on a genetic type (genetically pure lines, populations, hybrids, pure varieties and clones. The key role of seeds in preserving a plant variety, or a cultivar, is emphasized. In the article, cultivar and yield qualities of seeds are characterized. The reasons for seed deterioration and yield decrease have been looked into. Improvement of main crops such as wheat, barley, rye, rice, soybeans, maize, triticale, sunflowers, potatoes, sugar beets is characterized in detail. The author shares the traditional point of view on the seed breeding program. It includes following steps: development phase (breeder’s seed, maintenance phase (in which a quantity of seed is maintained under high standards and the distribution phase (production of commercial seed for distribution to the public. The difference between primary and secondary seed selection is explained. The best periods for crop rotation and crop renovation are also described. The legally enforceable standards of quality and genetic purity for different crops are presented in the article. It is pointed out that it is vital to maintain high cultivar qualities after multiplication and distribution of the new variety. Only large-scale pedigree system for seed production promotes it

  10. Physicochemical and grinding characteristics of dragonhead seeds

    Dziki, D.; Mi?, A.; G?adyszewska, B.; Laskowski, J.; Kwiatkowski, S.; Gawlik-Dziki, U.

    2013-12-01

    The results of investigations on the physicochemical and grinding characteristics of Moldavian dragonhead seeds are presented. The data obtained showed that the physical properties (average size, sphericity, surface area, 1 000 seed mass, dynamic angle of repose, coefficient of static friction, and bulk and true densities) of the white and blue form of dragonhead seeds were not significantly different. Both forms of dragonhead showed similar levels of protein (average of 21%), whereas the blue form of dragonhead had a lower fat content (average of 23.1%) and higher mucilage content (13.35%) in comparison to the blue form of seed (24.6 and 9.95%, respectively). Antioxidant capacity of dragonhead seeds was comparable for both forms and averaged about 40%, which corresponded to EC50 values of 0.12 and 0.13 mgml-1 for the white and blue forms, respectively. The grinding material showed similar particle size distribution of ground material for both forms of dragonhead seeds. The lowest values of specific grinding energy were obtained for the white form of dragonhead seeds, those for the blue form were significantly higher. Grinding of dragonhead seeds using a screen 2.0 mm mesh size caused screen hole clogging. This problem did not occur when dragonhead seeds were mixed with wheat grain.

  11. Inheritance of seed coat color in sesame

    Hernán Laurentin

    2014-04-01

    Full Text Available The objective of this work was to determine the inheritance mode of seed coat color in sesame. Two crosses and their reciprocals were performed: UCLA37 x UCV3 and UCLA90 x UCV3, of which UCLA37 and UCLA90 are white seed, and UCV3 is brown seed. Results of reciprocal crosses within each cross were identical: F1 seeds had the same phenotype as the maternal parent, and F2 resulted in the phenotype brown color. These results are consistent only with the model in which the maternal effect is the responsible for this trait. This model was validated by recording the seed coat color of 100 F2 plants (F3 seeds from each cross with its reciprocal, in which the 3:1 expected ratio for plants producing brown and white seeds was tested with the chi-square test. Sesame seed color is determined by the maternal genotype. Proposed names for the alleles participating in sesame seed coat color are: Sc1, for brown color; and Sc2, for white color; Sc1 is dominant over Sc2.

  12. Weed Seed Labelling with Rare Earth Elements

    The dormancy period of weed seed under field conditions so far could not be determined with reliability because of the lack of a suitable method for labelling seed. Rademacher and co-workers labelled weed seed with 35S for studying germination in relation to different methods of soil cultivation. Since 35S has a half-period of 87.1 d only, labelling is practically ineffective after one year. The authors of this paper therefore tried to label weed seed with lanthanum, europium and dysprosium. Since these elements (1) do not occur in the standard soils in interfering quantities, and (2) can easily be determined by neutron activation analysis, they are useful for non-radioactive labelling. In pot experiments they were added like fertilizers before the soil was seeded with Sinapsis alba or Vicia villosa. After harvesting, samples of the plants' roots, stems, leaves, and seed were irradiated in the reactor and the rare earth elements determined by neutron-activation analysis. Evaluation of the results disclosed that traceable quantities of lanthanum, europium, and dysprosium were present in roots, stems, and leaves, but not in the grains of seed. It is the intention, in further experiments, to increase the concentration of the applied elements up to the limit of compatibility, and to find out whether a more concentrated supply will increase the incorporation in seeds. If one of these elements is present in a reasonable concentration, experiments with labelled weed seed will be possible for periods of several years. (author)

  13. Phase sensitive Raman process with correlated seeds

    Chen, Bing; Qiu, Cheng; Chen, L. Q., E-mail: lqchen@phy.ecnu.edu.cn; Zhang, Kai; Guo, Jinxian; Yuan, Chun-Hua; Zhang, Weiping [Quantum Institute for Light and Atoms, State Key Laboratory of Precision Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062 (China); Ou, Z. Y. [Quantum Institute for Light and Atoms, State Key Laboratory of Precision Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062 (China); Department of Physics, Indiana University-Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, Indiana 46202 (United States)

    2015-03-16

    A phase sensitive Raman scattering was experimentally demonstrated by injecting a Stokes light seed into an atomic ensemble, whose internal state is set in such a way that it is coherent with the input Stokes seed. Such phase sensitive characteristic is a result of interference effect due to the phase correlation between the injected Stokes light field and the internal state of the atomic ensemble in the Raman process. Furthermore, the constructive interference leads to a Raman efficiency larger than other kinds of Raman processes such as stimulated Raman process with Stokes seed injection alone or uncorrelated light-atom seeding. It may find applications in precision spectroscopy, quantum optics, and precise measurement.

  14. Patogenic fungi associated with blue lupine seeds

    Bogdan Nowicki

    2013-12-01

    Full Text Available Over 10% ofseeds harvested in 1991 and 1992 (50 samples, 400 seeds in each sample proved to be infested with various fungi. Fusarium spp. and Botrytis cinerea were the most common pathogens isolated. Fusarium avenaceum was the most common and highIy pathogenic species. Fusarium semitectum and F. tricinctum were highly pathogenic to lupin seedlings but they were the least common Fusarium isolated from seeds. Similarily, Sclerotinia sclerotiorum was isolated only from 0,2% seeds tested but this fungus was highly pathogenic to lupin seedlings. Some other fungi know as lupin pathogens (F. oxysporum, Stemphylium botryosum, Pleiochaeta setosa and Phomopsis leptostromiformis were also noted in tested seeds.

  15. Mycoflora in Exhumed Seeds of Opuntia tomentosa and Its Possible Role in Seed Germination

    Yadira Olvera-Carrillo; Silvia Espinosa-Matías; Margarita Villegas; Guadalupe Vidal-Gaona; Jeanette Rosas-Moreno; Judith Márquez-Guzmán; María Esther Sánchez-Coronado; Alma Orozco-Segovia

    2011-01-01

    The funicular cover of the Opuntia tomentosa seed limits imbibition; germination occurs only when the funicle is weakened or the funicular valve is removed. We investigated the role of fungi in funicular weakening and seed germination. Seeds that had been either buried in one of two sites or stored in the laboratory were germinated with and without a valve. Disinfected or nondisinfected seeds and their naked embryos were cultivated on agar or PDA. None of the 11 identified fungal genera grew ...

  16. Parasitoid wasps indirectly suppress seed production by stimulating consumption rates of their seed-feeding hosts.

    Xi, Xinqiang; Eisenhauer, Nico; Sun, Shucun

    2015-07-01

    In parasitoid-herbivore-plant food chains, parasitoids may be simultaneously linked with both herbivore hosts and plants, as occurs when herbivores attacked by parasitoids continue to consume plants although they are destined to die. This peculiar property may cause parasitoids to confer a differential trophic cascading effect on plants than that known for typical predators. We hypothesized that larval koinobiont parasitoids would confer an immediate negative effect on plant seed production by stimulating consumption of their seed-predator hosts. We tested this hypothesis in an alpine parasitic food chain of plant seeds, pre-dispersal seed predators (tephritid fly larvae) and koinobiont parasitoids using field observations, a field experiment and a microcosm study. We first compared observed seed production in (i) non-infected capitula, (ii) capitula infected only by seed predators (tephritid flies) and (iii) capitula infected by both seed predators and their parasitoids in five Asteraceae species. Consistent with our hypothesis, seed loss in the capitula with both seed predators and parasitoids was significantly greater than in the capitula infested only by seed predators. This effect was replicated in a controlled field experiment focusing on the most common parasitoid-seed predator-plant interaction chain in our system, in which confounding factors (e.g. density and phenology) were excluded. Here, we show that parasitoids indirectly decreased plant seed production by changing the behaviour of seed predators. In a microcosm study, we show that larval parasitoids significantly extended the growth period and increased the terminal size of their host tephritid maggots. Thus, parasitoids suppressed plant seed production by stimulating the growth and consumption of the fly maggots. In contrast to the typical predator-induced trophic cascade, we highlight the significance of parasitoids indirectly decreasing plant fitness by stimulating consumption by seed predators. Future studies on trophic interactions should consider the net effect of both increased consumption by seed predators and their death after development of parasitoids. PMID:25803150

  17. Seed Security: Exploring the Potential for Smallholder Production of Certified Seed Crop at Household Level

    N. Munyaka; B. M. Mvumi; U. M. Mazarura

    2015-01-01

    Most smallholder farmers in sub-Saharan Africa face food and nutrition security challenges arising from small land size, unavailability of, and poor access to quality seed, inter alia. A survey was conducted in the Zaka district of Masvingo province in Zimbabwe, to investigate the factors that limit smallholder farmers’ ability to produce, store or sell certified seed. The factors considered were the availability of certified seed, the appropriateness of storage facilities for seed storage at...

  18. Lipids in citrus sinensis seeds

    The seed oil of citrus sinensis when subjected to different physicochemical tests showed moisture 13.2%, ash 7.5%, ester value 1.29%, free fatty acid 0.4%. iodine value 65.0% and protein value 6.0%. According to lipid analysis. the oil was classified into hydrocarbons. wax esters, sterol esters, triglycerides. free fatty acids, 1,3 and 1,2 diglycerides, alcohols, sterols, monoglycerides, phosphatidylethanolamines, phosphatidylcholines and lysophosphatidylethanolamines. The fatty acid (C/sub 12.0/ - C/sub 21.0/) composition of all lipid classes was determined with the help of thin layer and gas liquid chromatography. (author)

  19. The seeded growth of graphene

    Lee, Jae-Kap; Lee, Sohyung; Kim, Yong-il; Kim, Jin-Gyu; Min, Bong-Ki; Lee, Kyung-Il; Park, Yeseul; John, Phillip

    2014-01-01

    In this paper, we demonstrate the seeded growth of graphene under a plasma chemical vapor deposition condition. First, we fabricate graphene nanopowders (~5 nm) by ball-milling commercial multi-wall carbon nanotubes. The graphene nanoparticles were subsequently subject to a direct current plasma generated in a 100 Torr 10%CH4 - 90%H2 gas mixture. The plasma growth enlarged, over one hour, the nuclei to graphene sheets larger than one hundred nm2 in area. Characterization by electron and X-ray...

  20. SEED LONGEVITY CHART TO PREDICT VIABILITY OF CORN SEED DURING OPEN STORAGE

    CLAUDINEI ANDREOLI

    2007-08-01

    Full Text Available The simplified equation Vt = Vi – tg.p is to predict the percentage of viability of any corn (Zea mays L. seed lot after any period of time under open storage conditions. Based upon that equation, the objective of this work was to set a practical seed longevity chart to predict viability of corn seed lot during storage. From data of five corn seed lots stored at Sete Lagoas, MG, the value of the storage index ( was calculated. This value ( = 120 days corresponded exactly to the time taken in days for the initial germination of corn seeds stored to fall to a certain germination value observed in the accelerated aging test. Once the storage index ( is estimated, the viability chart which illustrates the relationship between initial germination, seed deterioration rate and storage condition over time can be constructed. The chart can be used by any seed producer once the value of storage index ( in situ is determined. The application of the seed viability chart to short-term seed storage seems to be promising for seed growers. This predictive model is the first attempt of incorporating the effect of seed quality, the deterioration rate and the storage environmental conditions (temperature and relative humidity into a seed longevity chart of an open storage.

  1. Loose seeds versus stranded seeds in I-125 prostate brachytherapy: Differences in clinical outcome

    Purpose: To assess clinical outcome in terms of biochemical No evidence of disease (bNED) for patients with stranded seed implants versus loose seed implants in prostate brachytherapy. Methods: From December 2000 until October 2006, we treated 896 T≤2C Nx/0 Mx/0, prostate cancer patients with either stranded seed (n = 538) or loose seed (n = 358) I-125 implants. A total of 211 patients received a 6 months course of anti-androgen therapy, before treatment, for prostate volume reduction to <50 cc. Patients with very small and large gland volumes or a history of transurethral prostate resection, were preferably treated with stranded seeds, otherwise selection was arbitrary. Results: The 5-year bNED rates (95% Confidence Interval) for stranded seed patients and loose seed patients were respectively 86% (82-90) and 90% (85-95), the total 5-year bNED rate was 87% (85-90). When adjusted for possible confounding factors in a Cox-regression analysis, type of seed was significantly associated with biochemical failure with a 43% risk reduction (hazard ratio: 0.57; 95% CI: 0.34-0.97) for loose seeds versus stranded seeds. Conclusions: These results suggest that seed-type affects clinical outcome in prostate brachytherapy, with better bNED for patients with loose seed implants.

  2. Chlorophyll fluorescence of the testa of Brassica oleracea seeds as an indicator of seed maturity and seed quality

    Jalink H.

    1998-01-01

    Full Text Available Chlorophyll fluorescence of the testa of seeds is proposed as a non-invasive method for the determination of maturity and quality of seeds. In this study cabbage seeds (Brassica oleracea were sorted individually based on the chlorophyll fluorescence signals into four subsamples labeled with respect to their chlorophyll fluorescence signal (low, medium, high and very high. The results show that the magnitude of the chlorophyll fluorescence signal was inversely related to the quality of the seeds, expressed as germination %, normal seedling %, germination rate (T50 and uniformity of germination (T75-T25. The seed lot could be improved from 90 to 97% normal seedlings by sorting out 13% of the seeds with very high chlorophyll fluorescence signals. Advantages of the chlorophyll fluorescence method for sorting seeds are the high sensitivity, the method being fully non-destructive, the high speed at which the fluorescence is generated and measured and the specificity for only chlorophyll. Other pigments or substances which can influence seed colour but do not fluoresce at the specific wavelengths of excitation and emission of chlorophyll, will not contribute to the fluorescence signal. These characteristics make chlorophyll fluorescence highly suitable as a new sorting technique.

  3. Formulated diets versus seed mixtures for psittacines.

    Ullrey, D E; Allen, M E; Baer, D J

    1991-11-01

    Psittacines are often classified as seed eaters despite studies that have established great diversity in food habits in the wild. While seeds are consumed, so are flowers, buds, leaves, fruits and cambium. Some psittacines consume part of greater than 80 species of grasses, forbs, shrubs and trees. In addition, insects may be important. Although there are few controlled studies of the requirements of psittacines, it is probable that most nutrient needs are comparable to those of domesticated precocial birds that have been thoroughly studied. Commercial seed mixes for psittacines commonly contain corn, sunflower, safflower, pumpkin and squash seeds, wheat, peanuts, millet, oat groats and buckwheat, although other seeds may be present. Because hulls/shells comprise 18-69% of these seeds and they are removed before swallowing, a significant proportion of typical seed mixtures is waste. Some of the seeds also are very high in fat and promote obesity. Common nutrient deficiencies of decorticated seeds include lysine, calcium, available phosphorus, sodium, manganese, zinc, iron, iodine, selenium, vitamins A, D, E and K, riboflavin, pantothenic acid, available niacin, vitamin B-12 and choline. Attempts to correct these deficiencies by incorporating pellets into seed mixes are usually thwarted by rejection of the pellets and disproportionate consumption of items that are more highly favored. An extruded diet formulated to meet the projected nutrient needs of psittacines was fed with fruits and vegetables to eight species of psittacines for 1 y. Fledging percentage was increased to 90% from the 66% observed during the previous 2 y when these psittacines were fed seeds, fruits and vegetables. Although this extruded diet was well accepted in a mixture of fruits and vegetables and met nutrient needs, analyses have shown that not all commercial formulated diets are of equal merit. PMID:1941226

  4. Prostate brachytherapy seed localization by analysis of multiple projections: Identifying and addressing the seed overlap problem

    Intraoperative three-dimensional reconstruction of seed locations during prostate brachytherapy for purposes of immediate computation of radiation dosimetry is an active area of current investigation, including methods which use multiple fluoroscopic projections. A simulation study using seed locations extracted from clinical CT data was performed; the result showed that on average one quarter of the seeds had a projection image overlapping with other seeds. The average percentage of non-overlapping seeds for the prostate implants and seed types investigated was 74.5% with a range of 56.9%-92.9%. The distribution of seeds in different cluster sizes was analyzed as well as the distribution of pixel counts of connected components. A statistical classifier was developed to determine the number of seed images in a self-connected component in the segmented images. The classifier was tested with simulation data, and the error rate was below 2%. A method to determine seed image position is also provided. A modified three-film technique was used to reconstruct 3-D seed locations. The algorithm allows unequal number of seed images for each projection as input while current methods require the same number of seed images detected in all projections. An accuracy analysis based on angular and positional uncertainty was performed. The reconstruction and seed localization algorithms were tested with simulation data, and the mean distance error of the reconstructed results was 0.61 mm. A phantom study was performed to validate the seed localization method. Three false positive seeds, 4.7% of the total, in the reconstruction result were observed in this study

  5. Thermoperiodism in cocklebur seed germination.

    Esashi, Y; Tsukada, Y

    1978-03-01

    Germination potential in nondormant, upper cocklebur (Xanthium pensylvanicum Wallr.) seeds, which were incapable of germinating under constant temperatures below 25 C in air, was increased by exposure to diurnally alternating temperatures. The cocklebur seeds failed to respond to the temperature fluctuations in the beginning of water imbibition, and their responsiveness appeared only after aerobic presoaking for a limited period or after anaerobic pretreatment for 1 to 3 days.Maximal germination was obtained after exposure to a thermoperiodic regime of 8 hours at 23 C and 16 hours at 8 C. A process occurring during the high temperature phase was aerobic and had to precede the inductive low temperature phase, its effect increasing with temperature. Critical minimum length of the inductive low temperature phase changed with the duration of a preceding anaerobiosis, for instance about 4 hours after 1 day anaerobiosis, but about 2 hours after 2 days. Percentage of subsequent germination was in proportion to the number of thermoperiodic cycles. A process of the inductive low temperature phase was not perturbed by inserting a brief higher temperature period into its phase; indeed, such insertion rather increased germination potential when performed in the earlier parts of the inductive low temperature phase. The effect of the low temperature survived for 13 to 17 hours during the higher temperature period. PMID:16660310

  6. EFFECTS OF FUNGICIDES ON ALFALFA SEED YIELD

    Svetislav Popović

    2000-12-01

    Full Text Available Alfalfa is the most seeded forage crop in the Republic of Croatia.The seed requirements are estimated at 250-300 thousand kg each year. Despite the relatively suitable management, favourable environmental conditions and numerously recognized national cultivars the state seed production is insufficient and more than 70% of seed requirements are compensated by import. The research of a fungicides efficiency on alfalfa seed yield components (plant height, branch number, number of seeds per pod, number and weight of pods per stem, number and weight of seeds per stem, weight of 1000 seeds has been carried out during 1994 and 1995 in Osijek. It involved application of two fungicides (both of them without a state alfalfa application licence: Impact C (flutriafol+carbendazim and Tilt 250 EC (propiconazol on alfalfa second growth seed stands in a different phenological stages of alfalfa development. The experiment was established at two three year old alfalfa stands of cultivar Osječka 66 sown in thick rows (0.12 m row spacing and wide rows (0.5m row spacing. The experimental design was split-plot with four replicates. The plot size was 25 m2. The fungicide application variants were as follows: 1.non treated; 2. at the beginning of a flowering stage; 3. in a full flowering stage; 4.at the end of a flowering stage; 5. as a combination of 2+4; and 6.as a combination 3+4 in a dose: Impact C 0.5 l/ha / 400 l of water and Tilt 250 EC 1 l/ha / 400 l of water. A control of the seed stands health during the vegetation confirmed the presence of disease agents: Phoma medicaginis, Stemphylium botryosum, Pseudopeziza medicaginis and Uromyces striatus. The results of the investigation indicate that applied fungicides did not significantly affect alfalfa seed yield. The total average seed yield in 1994 was 323 kg/ha and in 1995 213 kg/ha. In 1994 the seed yield on fungicide application variant 2 was 381.88 kg/ha and it was by 35% higher than on variant 3 and by 23% higher than on variant 4. In that year of the investigation the highest seed yielding variant was variant 2 in a wide row sown stand (456.3 kg/ha. It was by 48.4% higher than the same variant in a thick row sown stand (307.5 kg/ha and by 27,5% higher than variant 1 (357.85 kg/ha. Also variant 2 had the highest number of seeds per pod and lowest height of plants in 1994 and the highest number and weight of pods in 1995. Both fungicides had significant effect (P<0.01 on weight of 1000 seeds in 1995. Row space didn't significantly influence seed yield in 1994, but it significantly affecteded it in 1995 (P<0.05. The higher yield had a thick row sown stand. During the two year sinvestigations wide rows sown stand had by 13.8% grater height of plants, by 31.2% larger number of branches, by 15.1% higher weight of seeds and by 7% larger number of pods and seeds per stem, but it hadn't higher seed yield mostly because unfavourable environmental conditions. Concerning results and new knowledge it is recommendable to continue the investigations and to check up an economic return of fungicides application in alfalfa seed stands.

  7. 7 CFR 201.47a - Seed unit.

    2010-01-01

    ...) One- and two-seeded pods of small-seeded legumes (Leguminosae), burs of the burclovers (Medicago... small-seeded legumes for purposes of identification.) Pods of legumes normally containing more than...

  8. Reasoned opinion on the modification of the existing MRLs for bixafen in rape seed, linseed, poppy seed and mustard seed

    European Food Safety Authority

    2013-02-01

    Full Text Available In accordance with Article 6 of Regulation (EC No 396/2005, the United Kingdom, hereafter referred to as the evaluating Member State (EMS, received an application from Bayer CropSciences AG to modify the existing MRL for the active substance bixafen in rape seed. The submitted residue data support the intended use of bixafen in rape seed, linseed, poppy seed and mustard seed in France as well. In order to accommodate for the intended use of bixafen, the United Kingdom proposed to raise the existing MRL from 0.015 mg/kg to 0.1 mg/kg. The United Kingdom drafted an evaluation report in accordance with Article 8 of Regulation (EC No 396/2005 which was submitted to the European Commission and forwarded to EFSA. According to EFSA the data are sufficient to derive a MRL proposal of 0.07 mg/kg for the proposed use on rape seed, linseed, poppy seed and mustard seed. An adequate analytical enforcement method is available to control the residues of bixafen in oilseeds. Based on the risk assessment results, EFSA concludes that the proposed use of bixafen on rape seed, linseed, poppy seed and mustard seed will not result in a consumer exposure exceeding the toxicological reference values and therefore is unlikely to pose a consumer health risk. This risk assessment has to be regarded as provisional since the residue definition for enforcement and risk assessment in oilseeds are provisional and pending the need for MRLs in rotational crops and the definitive MRLs in animal origin commodities.

  9. Tea seed upgrading facilities and economic assessment of biodiesel production from tea seed oil

    Green tea seed (Camellia sinensisL. Kuntze) oil was used in this work. The tea seed oil contains more than 84% unsaturated fatty acid, such as oleic acid (62.5% by weight), linoleic acid (18.1% by weight) and linolenic acid. The biodiesel from tea seed oil in itself is not significantly different from biodiesel produced from vegetable oils. However, tea seed oil has lower pour point and lower viscosity as different common vegetable oils. Crude tea seed oil is one of the cheapest vegetable oil feedstocks with average price, 514 (US$/ton).

  10. The effect of tuber seed cutting, tuber seed storage temperature and nitrogen fertilization in potato production

    Martins, Fernando; Rodrigues, M. A.; Coutinho, João

    1999-01-01

    In the present work, two treatments of die seed tuber, seed cutting and seed storage temperature were studied, with four levels of nitrogen applied once, at planting time. The cut seed gave a better yield in the medium size tubers, 37.8 T/ha and the non-refrigerated seed give a slight increased yield iii this class. The response to nitrogen was better, with 150 kg/ha giving the higher total yield and the higher yields of the two largest tuber classes considered. The effect of cutting the s...

  11. Seed Maturation Regulators Are Related to the Control of Seed Dormancy in Wheat (Triticum aestivum L.)

    Rikiishi, Kazuhide; Maekawa, Masahiko

    2014-01-01

    In Arabidopsis, the regulation network of the seed maturation program controls the induction of seed dormancy. Wheat EST sequences showing homology with the master regulators of seed maturation, LEAFY COTYLEDON1 (LEC1), LEC2 and FUSCA3 (FUS3), were searched from databases and designated respectively as TaL1L (LEC1-LIKE), TaL2L (LEC2-LIKE), and TaFUS3. TaL1LA, TaL2LA and TaFUS3 mainly expressed in seeds or embryos, with the expression limited to the early stages of seed development. Results sh...

  12. Tea seed upgrading facilities and economic assessment of biodiesel production from tea seed oil

    Green tea seed (Camellia sinensis L. Kuntze) oil was used in this work. The tea seed oil contains more than 84% unsaturated fatty acid, such as oleic acid (62.5% by weight), linoleic acid (18.1% by weight) and linolenic acid. The biodiesel from tea seed oil in itself is not significantly different from biodiesel produced from vegetable oils. However, tea seed oil has lower pour point and lower viscosity as different common vegetable oils. Crude tea seed oil is one of the cheapest vegetable oil feedstocks with average price, 514 (US$/ton). (author)

  13. Preliminary Studies on the Characterization of Orange Seed and Pawpaw Seed Oils

    J.N. Ihedioha; C.N. Ibeto; C.O.B. Okoye

    2011-01-01

    This study was carried out to determine the physicochemical properties of seeds and oil extracted from both papaya and orange seeds. The seeds of papaya and orange are generally discarded. However, in order to make a more efficient use of papaya and orange, it is worth investigating the use of the seeds as a source of oil. The seeds were collected from homes and the oils obtained by solvent extraction were analysed for pH, moisture content, specific gravity, refractive index, saponification v...

  14. Effect of heat stress during seed development and maturation on wheat (Triticum durum) seed quality. I. Seed germination and seeding vigor

    Grass, L. [Institut de la recherche agronomique, CRRA Settat, Settat (Morocco); Burris, J.S. [Iowa State Univ. of Science and Technology, Ames, IA (United States). Agricultural Experiment Station

    1995-10-01

    Two wheat cultivars were subjected to three temperature regimes beginning 10 days after anthesis to maturity. They were grown in three growth chambers, with 30 pots per chamber, 15 pots per variety. The experiments were replicated three times and temperature combinations were rotated among the three chambers. Two weeks after emergence, plants were thinned to three per pot with the tillers removed to keep only the main stem. High temperature resulted in low values of both seed yield and and physical traits of seed quality. However, the effect of temperature on seed germination was not consistent among the two cultivars: high temperature had no effect on one, but decreased seed germination of the other one. Seed vigor was adversely affected by heat stress: reduced shoot and root dry weight was observed, as well as reduced root length and low root number per seeding. . Exposing seeds to high temperature during development and maturity resulted in low embryo oxygen uptake. The study showed that the growing conditions (i.e. temperature) of the parent plant affect the quality of its seed. 36 refs., 5 figs., 2 tabs.

  15. Elastohydrodynamic Traction Properties of Seed Oils

    The elastohydrodynamic traction coefficient (tc) properties of nine seed oils of varying chemical structures, PAO and hexadecane, were investigated using a ball-on disk traction apparatus. The seed oils were: castor oil, a triglyceride with hydroxyl functional group; jojoba, a monoglyceride; and s...

  16. Elastohydrodynamic (EHD) traction properties of seed oils

    The elastohydrodynamic traction coefficient (tc) properties of nine seed oils of varying chemical structures, PAO and hexadecane, were investigated using a ball-on disk traction apparatus. The seed oils were: castor oil, a triglyceride with hydroxyl functional group; jojoba, a monoglyceride; and sev...

  17. Photomanipulation of phytochrome in lettuce seeds.

    Kendrick, R E; Russell, J H

    1975-08-01

    Seeds of lettuce (Lactuca sativa L. cv. Grand Rapids) were imbibed and given either short irradiation with red or far red light prior to drying or dried under continuous red or far red light. Seeds treated with either short or continuous red germinate in darkness, whereas seeds treated with either short or continuous far red require a short exposure to red light, after a period of imbibition, to stimulate germination. Irradiation of dry red seeds with far red light immediately before sowing results in a marked inhibition of germination. This result was predicted since far red-absorbing form phytochrome can be photoconverted to the intermediate P650 (absorbance maximum 650 nm) in freeze-dried tissue. A similar far red treatment to continuous red seeds is less effective and it is concluded that in these seeds a proportion of total phytochrome is blocked as intermediates between red-absorbing and far red-absorbing form phytochrome, which only form the far red-absorbing form of phytochrome on imbibition. The inhibition of dry short red seeds by far red light can be reversed by an irradiation with short red light given immediately before sowing, confirming that P650 can be photoconverted back to the far red-absorbing form of phytochrome. The results are discussed in relation to seed maturation (dehydration) on the parent plant. PMID:16659296

  18. Fungal endophytes in green coffee seeds

    Green coffee seeds from Colombia, Guatemala, India, Kenya, Papua New Guinea, Puerto Rico, and Vietnam were sampled for the presence of fungal endophytes. Sections of surface sterilized seeds were plated on yeast malt agar, and fungal growth was isolated for subsequent DNA extraction and sequencing....

  19. Study of seed for synthetical quartz

    Natural quartz blocks for seed (synthetic quartz technology) were studied by using various characterization techniques, such as X-ray topography, optical micrography, inspectoscopy, polariscopy and conoscopy, and etching. One of the most commonly found defect is the electrical or Dauphine twin. In The present research, we have developed a methodology to obtain a highly perfect seed for the synthetic quartz industries. (author)

  20. Iodine-125 seeds for cancer treatment

    In Brazil, cancer has become one of the major public health problems. An estimate by the Health Ministry showed that 466,430 people had the disease in the country in 2008. The prostate cancer is the second largest death cause among men. The National Institute of Cancer estimated the occurrence of 50,000 new cases for 2009. Some of these patients are treated with Brachytherapy, using Iodine-125 seeds. By this technique, small seeds with Iodine-125, a radioactive material, are implanted in the prostate. The advantages of radioactive seed implants are the preservation of healthy tissues and organs near the prostate, besides the low rate of impotence and urinary incontinence. The Energy and Nuclear Research Institute - IPEN, which belongs to the Nuclear Energy National Commission - CNEN, established a program for the development of the technique and production of Iodine-125 seeds in Brazil. The estimate for the 125-Iodine seeds demand is of 8,000 seeds/month and the laboratory to be implanted will need this production capacity. The purpose of this paper is to explain the project status and show some data about the seeds used in the country. The project will be divided in two phases: technological development of a prototype and a laboratory implementation for the seeds production. (author)

  1. SUPERMASSIVE SEEDS FOR SUPERMASSIVE BLACK HOLES

    Recent observations of quasars powered by supermassive black holes (SMBHs) out to z ?> 7 constrain both the initial seed masses and the growth of the most massive black holes (BHs) in the early universe. Here we elucidate the implications of the radiative feedback from early generations of stars and from BH accretion for popular models for the formation and growth of seed BHs. We show that by properly accounting for (1) the limited role of mergers in growing seed BHs as inferred from cosmological simulations of early star formation and radiative feedback, (2) the sub-Eddington accretion rates of BHs expected at the earliest times, and (3) the large radiative efficiencies ? of the most massive BHs inferred from observations of active galactic nuclei at high redshift (? ?> 0.1), we are led to the conclusion that the initial BH seeds may have been as massive as ?> 105 M?. This presents a strong challenge to the Population III seed model, which calls for seed masses of ?100 M? and, even with constant Eddington-limited accretion, requires ? ?4-106 M? seed BH. These results corroborate recent cosmological simulations and observational campaigns which suggest that these massive BHs were the seeds of a large fraction of the SMBHs residing in the centers of galaxies today.

  2. Bold seeded mutant in black gram

    Seeds of black gram (Vigna mungo (L.) Hepper) were irradiated with gamma rays and treated with EMS. A bold-seeded mutant was selected in the M2 generation, and the M3 generation showed more vigorous growth, an increase in height and leaf number and more pods per plant. 3 refs, 1 tab

  3. Stimulation of seeds by low dose irradiation

    The first section of the bibliography lists materials on the stimulation of seeds by low dose irradiation, with particular reference to stimulation of germination and yield. The second section contains a small number of selected references on seed irradiation facilities. (author)

  4. Policosanol content and composition in perilla seeds.

    Adhikari, Prakash; Hwang, Keum Taek; Park, Jae Nam; Kim, Choong Ki

    2006-07-26

    Policosanols, long-chain alcohols, have many beneficial physiological activities. Contents and compositions in perilla seeds (Perilla frutescens) produced in Korea and China were determined. Waxy materials were extracted from perilla seeds using hot hexane. Yield of the waxy materials from perilla seeds was 72.1 mg/100 g of dry weight. Contents and compositions of the waxy materials and policosanols were identified and quantified by TLC, HPLC, and GC. Major components of the waxy materials from Korean and Chinese perilla seeds were policosanols (25.5 and 34.8%, respectively), hydrocarbons (18.8 and 10.5%), wax esters, steryl esters and aldehydes (53.0 and 49.8%), acids (1.7 and 2.1%), and triacylglycerols (1.0 and 2.9%), determined by HPLC. For comparison, waxy materials of sesame seeds were also analyzed. Yield of the waxy materials from sesame seeds were 8.6 mg/100 g. Less than 5% policosanols were detected in the waxy materials extracted from sesame seeds produced in Korea and China. Wax esters or steryl esters accounted for 93-95% of the sesame waxy materials. Policosanols in the perilla seeds were composed of 67-68% octacosanol, 16-17% hexacosanol, 6-9% triacontanol, and others. PMID:16848517

  5. Convective Drying of Rapeseed Hybrids’ Seeds

    Željko Jukić

    2009-12-01

    Full Text Available The aim of this study was to investigate the water release from seeds of three rape hybrids, (‘Artus’, ‘Baldur’ and ‘Titan’, during the convection drying of the seeds at three different drying temperature (40, 60 and 80 °C. The drying was conducted in laboratory scale drier which can simulate the conditions of large drier. The air velocity in the drier was maintained at 1.0 m/s. Activation energy needed for starting the process of water release during the convective drying of oil seed rape seeds was also studied. According to the obtained values, the mathematical models (equations and curves of kinematic drying were determined. By comparing the exponential equations and the constants of water release from seeds it was observed that drying of hybrid Artus was the slowest and the one of hybrid Titan the fastest. Results showed that there was a significant difference between all investigated hybrids in water release rate and that within the same drying conditions, hybrids showed different behaviour. Moreover, air temperature increase caused significant increase in water release from the seeds. The highest activation energy needed for starting the process of water release from the seeds had the hybrid Artus and the lowest one hybrid Titan. Due to this, it can be concluded that the activation energy was reversely proportional to the water release rate from the seeds.

  6. Iodine-125 seeds for cancer treatment

    Rostelato, Maria E.C.M.; Zeituni, Carlos A.; Feher, Anselmo; Moura, Joao A.; Moura, Eduardo S.; Nagatomi, Helio R.; Manzoli, Jose E.; Souza, Carla D., E-mail: elisaros@ipen.b, E-mail: czeituni@pobox.co, E-mail: afeher@ipen.b, E-mail: jmoura31@yahoo.com.b, E-mail: esmoura@ipen.b, E-mail: hrnagato@ipen.b, E-mail: jemanzoli@ipen.b, E-mail: cdsouza@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Karam, Dib, E-mail: dib.karan@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Escola de Artes, Ciencias e Humanidades

    2009-07-01

    In Brazil, cancer has become one of the major public health problems. An estimate by the Health Ministry showed that 466,430 people had the disease in the country in 2008. The prostate cancer is the second largest death cause among men. The National Institute of Cancer estimated the occurrence of 50,000 new cases for 2009. Some of these patients are treated with Brachytherapy, using Iodine-125 seeds. By this technique, small seeds with Iodine-125, a radioactive material, are implanted in the prostate. The advantages of radioactive seed implants are the preservation of healthy tissues and organs near the prostate, besides the low rate of impotence and urinary incontinence. The Energy and Nuclear Research Institute - IPEN, which belongs to the Nuclear Energy National Commission - CNEN, established a program for the development of the technique and production of Iodine-125 seeds in Brazil. The estimate for the 125-Iodine seeds demand is of 8,000 seeds/month and the laboratory to be implanted will need this production capacity. The purpose of this paper is to explain the project status and show some data about the seeds used in the country. The project will be divided in two phases: technological development of a prototype and a laboratory implementation for the seeds production. (author)

  7. Treatments to break the dormancy of Cassia fistula L. seeds

    Paulo Alexandre Fernandes Rodrigues de Melo; Edilson Guedes da Costa; Sueli da Silva Santos-Moura; Edna Ursulino Alves; Roberta Sales Guedes

    2013-01-01

    Cassia fistula L. (Fabaceae – Caesalpinioidea) is a forest species that is propagated by seeds. To promote the production of seedlings of C. fistula it is necessary to break the natural dormancy of its seeds, which is caused by the seed tegument that is impermeable to water. The goal of this study was to establish a pre-germination treatment for germinating C. fistula seeds. The seeds were subjected to the following treatments: control – intact seeds (T1); mechanical scarification with sandpa...

  8. Effect of Physical Seed Sorting, Seed Treatment with Garlic Extract and Vitavax 200 on Seed Borne Fungal Flora and Seed Yield of Jute (Corchorus capsularis L.)

    S. M. A. Islam; Hossain, I.; G.A. Fakir; M. Asad-Ud-Doullah

    2001-01-01

    Effects of physical seed sorting, seed treatment with garlic extract (1:2) and Vitavax 200 (0.4%) were investigated for controlling seed borne fungal flora and seed yield of jute (Corchorus capsularis L.). Seed health test of jute revealed that farmer`s saved seed (control) yielded all together 13 different fungi of 11 genera. Prevalence of Colletotrichum corchori, Macrophomina phaseolina, Botryodiplodia theobromae, Fusarium spp., Penicillium spp., Aspergillus niger and A. flavus were by 4.25...

  9. Recovery and Germination of Grass Seeds Ingested by Cattle

    Ferhat Gökbulak

    2006-01-01

    Full Text Available Seeds of bluebunch wheatgrass [Psuedoroegneria spicata (Pursh A. Love] and Sandberg bluegrass (Poa secunda Presl. were fed to Holstein heifers in different amounts to investigate the effects of seed feeding level and seed size on the recovery and germinability of passed seed. Animals were fed 60,000, 30,000,15,000, and 7,500 seeds of each species. Passed seeds were recovered from dungcollected daily over a 4-day period and tested for germinability. In general, recovery of the larger-seeded bluebunch wheatgrass and the smaller-seeded Sandberg bluegrass declined as seed feeding levels decreased from 60,000 to 7,500 seeds per animal, and as time after seed ingestion increased from 1 to 4 days. Total seed recovery over the 4-day period was greater for bluebunch wheatgrass at the 60,000 seed feeding level, similar for both species at the 30,000 seed feeding level, and greater for Sandberg bluegrass at the 15,000 and 7,500 seed feeding levels.Germinability of bluebunch wheatgrass seeds decreased with each additional day in the digestive tract, while germinability of Sandberg bluegrass seeds remained constant or increased with time. Germinability of both species tended to increase as seed feeding levels decreased from 60,000 to 15,000 seeds per animal. Sandberg bluegrass seeds had greater germinability than bluebunch wheatgrass at all seed feeding levels and collection dates. Recovered seeds had significantly lower germinability than noningested seeds for both species. Results showed that livestock have the potential for dispersing enough germinable seeds on degraded rangelands.

  10. Seed deposition patterns and the survival of seeds and seedlings of the palm Euterpe edulis

    Pizo, Marco A.; Simão, Isaac

    2001-08-01

    The seed deposition pattern created by a seed disperser is one of the components of the efficiency of a species as seed disperser, and ultimately may influence the recruitment of a plant species. In this study, we used the seeds of a bird-dispersed forest palm, Euterpe edulis, to investigate the effects of two distinct seed deposition patterns created by birds that defecate (clumped pattern) and regurgitate seeds (loose-clumped pattern) on the survival of seeds experimentally set in an E. edulis-rich site, and of seedlings grown under shade-house conditions. The study was conducted in the lowland forest of Parque Estadual Intervales, SE Brazil. Clumped and loose-clumped seeds were equally preyed upon by rodents and insects. Although clumped and isolated seedlings had the same root weight after 1 year, the isolated seedlings survived better and presented more developed shoots, suggesting intraspecific competition among clumped seedlings. Our results indicate that animals that deposit E. edulis seeds in faecal clumps (e.g. cracids, tapirs) are less efficient seed dispersers than those that regurgitate seeds individually (e.g. trogons, toucans). Intraspecific competition among seedlings growing from faecal clumps is a likely process preventing the occurrence of clumps of adult palms.

  11. Florivory Modulates the Seed Number-Seed Weight Relationship in Halenia elliptica (Gentianaceae).

    Wang, Linlin; Meng, Lihua; Luo, Jian

    2015-01-01

    Generally, plant reproductive success might be affected negatively by florivory, and the effects may vary depending on the timing and intensity of florivory. To clarify the impacts of florivory by the sawfly larvae (Tenthredinidae) on seed production of Halenia elliptica D. Don, we simulated florivory by removing different proportion of flowers at three reproductive stages in this alpine herb and then examined the seed number per fruit, the seed weight, and the seed mass per fruit of the remaining flowers. Seed number per fruit reduced significantly when flowers were removed at flowering and fruiting stages or when 15% and 60% of flowers were removed. However, seed weight increased significantly after flowers were removed, independent of treatments of reproductive stage and proportion. There was a similar seed mass per fruit between the plants subjected to simulation of florivory and control. The results indicated that florivory modulated the seed number-seed weight relationship in this alpine species. Our study suggested that selective seed abortion and resource reallocation within fruits may ensure fewer but larger seeds, which were expected to be adaptive in the harsh environments. PMID:26495428

  12. Seed predators exert selection on the subindividual variation of seed size.

    Sobral, M; Guitián, J; Guitián, P; Larrinaga, A R

    2014-07-01

    Subindividual variation among repeated organs in plants constitutes an overlooked level of variation in phenotypic selection studies, despite being a major component of phenotypic variation. Animals that interact with plants could be selective agents on subindividual variation. This study examines selective pressures exerted during post-dispersal seed predation and germination on the subindividual variation of seed size in hawthorn (Crataegus monogyna). With a seed offering experiment and a germination test, we estimated phenotypic selection differentials for average and subindividual variation of seed size due to seed predation and germination. Seed size affects germination, growth rate and the probability of an individual seed of escaping predation. Longer seeds showed higher germination rates, but this did not result in significant selection on phenotypes of the maternal trees. On the other hand, seed predators avoided wider seeds, and by doing so exerted phenotypic selection on adult average and subindividual variation of seed size. The detected selection on subindividual variation suggests that the levels of phenotypic variation within individual plants may be, at least partly, the adaptive consequence of animal-mediated selection. PMID:24176051

  13. Use of molecular marker techniques in seed testing by Brazilian seed companies

    Della Vecchia P.T.

    1998-01-01

    Full Text Available Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

  14. Design of Millet Seed-metering Device under Hole-seeding Conditions

    Xiaoshun Zhao

    2015-09-01

    Full Text Available In order to solve the problem that traditional millet drilling method needs labor-intensive artificial thinning-out, a hole-seeding method is proposed to replace it. Under hole-seeding conditions, an air-suction millet seed-metering device was designed. The design of air-cutting valve can assure that suction-holes are not clogged and reliably falling seeds. Suction-hole structure of the seed-metering device takes chamfer type. The dimension parameter of the chamfer hole is as follows: diameter of 4 mm, chamfer depth of 2.5 mm, fissuriform bottom of suction-hole is 1 mm width, number of holes is 32. The passing rate of hill spacing, repeat-sowing rate, leak-sowing rate and hill formation were analyzed by seed-metering test platform, which meets the precision of millet seed and agronomic requirements.

  15. [Seeds latency of Stylosanthes hamata (Leguminosae) and its relation with morphology of the seed-coat].

    Castillo, R; Guenni, O

    2001-03-01

    The seed of Stylosanthes hamata is characterized by it's a high level of dormancy, which is imposed by seed-coat impermeability to water entry (harseededness or hard-seed condition). The level of hardseededness between upper and lower articulations of seven diploid and five tetraploid accessions was studied under tropical conditions. Germination tests on naked seeds (relative humidity: 55-65%, constant temperature: 27-30 degrees C, photoperiod: 12/12 hr) showed that hard-seed percentage was, in most cases, over 85% independent of ploidy level and seed position within the fruit. Histochemical analysis indicated that hardseededness in this species was associated with a completely sealed microphile and hilum. This feature was also associated to: a) an epidermis consisting of a palisade-like layer of macrosclereids, b) a like-line at the outermost region of these cells and c) a high concentration of hydrophobic compounds located all across the seed-coat. PMID:11795157

  16. Molecular regulation of seed and fruit set.

    Ruan, Yong-Ling; Patrick, John W; Bouzayen, Mondher; Osorio, Sonia; Fernie, Alisdair R

    2012-11-01

    Seed and fruit set are established during and soon after fertilization and determine seed and fruit number, their final size and, hence, yield potential. These processes are highly sensitive to biotic and abiotic stresses, which often lead to seed and fruit abortion. Here, we review the regulation of assimilate partitioning, including the potential roles of recently identified sucrose efflux transporters in seed and fruit set and examine the similarities of sucrose import and hydrolysis for both pollen and ovary sinks, and similar causes of abortion. We also discuss the molecular origins of parthenocarpy and the central roles of auxins and gibberellins in fruit set. The recently completed strawberry (Fragaria vesca) and tomato (Solanum lycopersicum) genomes have added to the existing crop databases, and new models are starting to be used in fruit and seed set studies. PMID:22776090

  17. Variability in seed characters of Tinospora cordifolia

    Anjali K Sharma

    2014-10-01

    Full Text Available Tinospora cordifolia, commonly known as Guduchi is a glabrous, deciduous climber which belongs to family Menispermaceae. Fruit is drupe,   ovoid, glossy and red. Investigation was carried out to know the extent of variability in  seed characters of 22 female accessions of T. cordifolia. Based on two years observations it was found wide  variability   of seed shape, seed surface pattern, seed size and condyle size existed among the accessions and these characters were found stable. Hence it is concluded that seed characters can be used for the characterization of accessions in T. cordifolia germplasm and these characters can be used in   DUS descriptors of the species.

  18. Asymbiotic in vitro seed propagation of Dendrobium.

    Teixeira da Silva, Jaime A; Tsavkelova, Elena A; Ng, Tzi Bun; Parthibhan, S; Dobránszki, Judit; Cardoso, Jean Carlos; Rao, M V; Zeng, Songjun

    2015-10-01

    The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro techniques for their asymbiotic seed germination. The influence of abiotic factors (such as temperature and light), methods of sterilization, composition of basal media, and supplementation with organic additives and plant growth regulators are discussed in context to achieve successful seed germination, protocorm formation, and further seedling growth and development. This review provides both a basis for the selection of optimal conditions, and a platform for the discovery of better ones, that would allow the development of new protocols and the exploration of new hypotheses for germination and conservation of Dendrobium seeds and seedlings. PMID:26183950

  19. Seeds of hope, seeds of despair: towards a political economy of the seed industry in southern Africa.

    Zerbe, N

    2001-01-01

    The seed industry in Southern Africa has been radically transformed by a policy of liberalisation and privatisation started under structural adjustment. Traditionally under the domain of parastatals, seed research, production and distribution has been criticised for failing to provide modern variety seed to smallholder farmers. However, the private companies which have stepped in to replace seed parastatals in southern Africa have proven no more effective in meeting the demands of smallholders. The Trade Related Intellectual Property Rights (TRIPs) Agreement, concluded in 1994 as part of the Uruguay Rounds of GATT negotiations, as well as certain biotechnological innovations such as Terminator or Traitor technologies, threaten to further undermine local seed production and consumption by destroying the informal seed sector so central to agricultural production in the region. What alternatives exist? The success of Zimbabwe's maize seed network offers some insight. Resting on a unique relationship between government and nationally based producer co-operatives, Zimbabwe's maize programme was able to provide nearly every farmer in the country with hybrid maize suited for local growing conditions. PMID:19205120

  20. Multiexciton Generation in Seeded Nanorods.

    Eshet, Hagai; Baer, Roi; Neuhauser, Daniel; Rabani, Eran

    2014-08-01

    The stochastic formulation of multiexciton generation (MEG) rates is extended to provide access to MEG efficiencies in nanostructures containing thousands of atoms. The formalism is applied to a series of CdSe/CdS seeded nanorod heterostructures with different core and shell dimensions. At energies above 3Eg (where Eg is the band gap), the MEG yield increases with decreasing core size, as expected for spherical nanocrystals. Surprisingly, this behavior is reversed for energies below this value, and is explained by the dependence of the density of states near the valence band edge, which increases with the core diameter. Our predictions indicate that the onset of MEG can be shifted to lower energies by manipulating the density of states in complex nanostructure geometries. PMID:26277946

  1. Seed rain, soil seed bank, seed loss and regeneration of Castanopsis fargesii (Fagaceae) in a subtropical evergreen broad-leaved forest

    Du, X.; Guo, Q.; Gao, X.; Ma, K.

    2007-01-01

    Understanding the seed rain and seed loss dynamics in the natural condition has important significance for revealing the natural regeneration mechanisms. We conducted a 3-year field observation on seed rain, seed loss and natural regeneration of Castanopsis fargesii Franch., a dominant tree species in evergreen broad-leaved forests in Dujiangyan, southwestern China. The results showed that: (1) there were marked differences in (mature) seed production between mast (733,700 seeds in 2001) and regular (51,200 and 195,600 seeds in 2002 and 2003, respectively) years for C. fargesii. (2) Most seeds were dispersed in leaf litter, humus and 0-2 cm depth soil in seed bank. (3) Frequency distributions of both DBH and height indicated that C. fargesii had a relatively stable population. (4) Seed rain, seed ground density, seed loss, and leaf fall were highly dynamic and certain quantity of seeds were preserved on the ground for a prolonged time due to predator satiation in both the mast and regular years so that the continuous presence of seed bank and seedling recruitments in situ became possible. Both longer time observations and manipulative experiments should be carried out to better understand the roles of seed dispersal and regeneration process in the ecosystem performance. ?? 2006 Elsevier B.V. All rights reserved.

  2. Determination Testing of Seed Hardness of Staple Breeding Wheat Seed in Gansu Province of China

    Fei Dai

    2015-02-01

    Full Text Available Seed hardness is one of the important indexes of grain classification. It has close relationship with grain powder, flour quality, seed storage and processing, resist insect pest and so on. In this study, which applied based on the grain hardness indentation loading curve method and chose 3 kind of staple breeding wheat seed to determine the seed hardness in Gansu province. The experimental results showed that the average hardness value of staple breeding wheat seed was 22.42 MPa~57.85 MPa and the dispersion of the hardness value was small, which also between 3~12%. The seed hardness of breeding wheat of Western drought-resistant No.1 was maximum (52.36~61.36MPa and the seed hardness of breeding wheat of Western drought-resistant No.3 between two parties of the other breeding wheat, which was 42.12~50.20MPa. The seed hardness of breeding wheat of Western drought-resistant No.2 was minimum, which were 58.10~62.91% and 47.91~54.66% lower than the rest of the two breeding wheat seed hardness respectively. The findings will provide theoretical basis for seed production and processing and the stimulation analysis by EDEM.

  3. Grazing impact on desert plants and soil seed banks: Implications for seed-eating animals

    Pol, Rodrigo G.; Sagario, M. Cecilia; Marone, Luis

    2014-02-01

    We assess whether the knowledge of livestock diet helps to link grazing effects with changes in plant cover and soil seed bank size, aiming at inferring the consequences of grazing on seed-eating animals. Specifically, we test whether continuous and heavy grazing reduce the cover, number of reproductive structures and seed reserves of the same grass species whose seeds are selected and preferred by granivorous animals in the central Monte desert, Argentina. Grass cover and the number of grass spikes usually diminished under grazing conditions in the two localities studied (Telteca and Ñacuñán), and soil seed bank was consistently reduced in all three years evaluated owing to a decline of perennial grass and forb seeds. In particular, the abundance of those seeds selected and preferred by birds and ants (in all cases grass species) declined 70-92% in Ñacuñán, and 52-72% in Telteca. Reduction of perennial grass cover and spike number in grazed sites reinforced the causal link between livestock grazing and the decline of grass soil seed reserves throughout failed plant reproduction. Grass seed bank depletion suggests that grazing may trigger a "cascade" of mechanisms that affect the abundance and persistence of valuable fodder species as well as the availability of seed resources for granivorous animals.

  4. A Linear Time Algorithm for Seeds Computation

    Kociumaka, Tomasz; Radoszewski, Jakub; Rytter, Wojciech; Walen, Tomasz

    2011-01-01

    Periodicity in words is one of the most fundamental areas of text algorithms and combinatorics. Two classical and natural variations of periodicity are seeds and covers (also called quasiperiods). Linear-time algorithms are known for finding all the covers of a word, however in case of seeds, for the past 15 years only an $O(n\\log{n})$ time algorithm was known (Iliopoulos, Moore and Park, 1996). Finding an $o(n\\log{n})$ time algorithm for the all-seeds problem was mentioned as one of the most important open problems related to repetitions in words in a survey by Smyth (2000). We show a linear-time algorithm computing all the seeds of a word, in particular, the shortest seed. Our approach is based on the use of a version of LZ-factorization and non-trivial combinatorial relations between the LZ-factorization and seeds. It is used here for the first time in context of seeds. It saves the work done for factors processed earlier, similarly as in Crochemore's square-free testing.

  5. Effects of gamma radiation in annatto seeds

    Franco, Camilo F. de Oliveira, E-mail: camilo.urucum@hotmail.com [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA/EMEPA), Joao Pessoa, PB (Brazil); Arthur, Valter; Arthur, Paula B., E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Harder, Marcia N.C., E-mail: marcia.harder@fatec.sp.gov.br [Centro Paula Souza, Curso Superior de Tecnologia em Bicombustiveis (FATEC), Piracicaba, SP (Brazil); Filho, Jose C.; Neto, Miguel B., E-mail: jorgecazefilho@yahoo.com.br [Empresa Estadual de Pesquisa Agropecuaria da Paraiba (EMEPA), Joao Pessoa, PB (Brazil)

    2015-07-01

    The annatto bixin has emerged as a major source of natural dyes used in the world notably by the substitution of synthetics harmful to human health and ecologic tendency in obtaining industrial products free of additives with applications in industries textiles; cosmetics; pharmaceutical and food mainly. The aim of this research was to obtain increased of germination rate and dormancy breaking on annatto seeds by gamma radiation. Annatto dry seeds were exposed to low doses of gamma radiation from source of Cobalt-60, type Gammecell-220, at 0.456 kGy/hour dose rate. In order to study stimulation effects of radiation on germination rate and dormancy breaking in the seeds. Five treatments with gamma radiation doses were applied as follows: 0 (control); 100; 125; 150 and 175 Gy. After irradiation the annatto seeds were planted as for usual seed production. According to the results obtained in this experiment we can conclude that the low doses of gamma radiation utilized on the annatto seeds did not presented significantly effect on the germination of plants. But the best dose to increase the germination of seeds was 150 Gy. (author)

  6. Sowing seasons and quality of soybean seeds

    Ávila Marizangela Rizzatti

    2003-01-01

    Full Text Available Considering the difficulties of producing high quality soybean [Glycine max (L. Merrill] seeds during the traditional cropping period in some areas of the State of Paraná, Brazil, a research project was carried out with the objective of evaluating the influence of sowing dates on the physiological and sanitary quality of seeds, during the 1998/99 and 1999/00 cropping seasons, in Maringá, PR, Brazil. The experiment consisted of five cultivar competition assays, arranged in a completely randomized block design, with each assay sown at different dates (10/15, 10/30, 11/15, 11/30 and 12/15 for each cropping season. The evaluated cultivars were BRS 132 (early, BRS 133 (semi-early, BR 16 (semi-early, BRS 134 (intermediate and FT- Estrela (late. Seeds obtained at the sowing dates were evaluated in the laboratory by germination, accelerated aging, and health tests. Sowing in November resulted in seeds with superior physiological and health quality. Cultivar BRS 133 showed the greatest stability in seed production with better quality for the different sowing dates. Cultivars BRS 134 and BRS 133, which were sown during the period from 10/15 to 11/30, produced seeds that had higher percentages of normal seedlings in the germination and accelerated aging tests. Advancing or delaying sowing dates had adverse effects on soybean seed production with regard to their sanitary quality.

  7. Effects of gamma radiation in annatto seeds

    The annatto bixin has emerged as a major source of natural dyes used in the world notably by the substitution of synthetics harmful to human health and ecologic tendency in obtaining industrial products free of additives with applications in industries textiles; cosmetics; pharmaceutical and food mainly. The aim of this research was to obtain increased of germination rate and dormancy breaking on annatto seeds by gamma radiation. Annatto dry seeds were exposed to low doses of gamma radiation from source of Cobalt-60, type Gammecell-220, at 0.456 kGy/hour dose rate. In order to study stimulation effects of radiation on germination rate and dormancy breaking in the seeds. Five treatments with gamma radiation doses were applied as follows: 0 (control); 100; 125; 150 and 175 Gy. After irradiation the annatto seeds were planted as for usual seed production. According to the results obtained in this experiment we can conclude that the low doses of gamma radiation utilized on the annatto seeds did not presented significantly effect on the germination of plants. But the best dose to increase the germination of seeds was 150 Gy. (author)

  8. Oxygen requirement of germinating flax seeds

    Kuznetsov, Oleg A.; Hasenstein, K. H.

    2003-05-01

    Plant experiments in earth orbit are typically prepared on the ground and germinated in orbit to study gravity effects on the developing seedlings. Germination requires the breakdown of storage compounds, and this metabolism depends upon respiration, making oxygen one of the limiting factors in seed germination. In microgravity lack of run-off of excess water requires careful testing of water dispensation and oxygen availability. In preparation for a shuttle experiment (MICRO on STS-107) we studied germination and growth of flax ( Linum usitatissimum L.) seedlings in the developed hardware (Magnetic Field Chamber, MFC). We tested between four to 32 seeds per chamber (air volume = 14 mL) and after 36 h measured the root length. At 90 μl O 2 per seed (32 seeds/chamber), the germination decreased from 94 to 69%, and the root length was reduced by 20%, compared to 8 seeds per chamber. Based on the percent germination and root length obtained in controlled gas mixtures between 3.6 and 21.6% O 2 we determined the lower limit of reliable germination to be 10 vol. % O 2 at atmospheric pressure. Although the oxygen available in the MFC's can support the intended number of seeds, the data show that seed storage and microgravity-related limitations may reduce germination.

  9. Seed ecology of Bromus sterilis L.

    Žďárková, Veronika

    2014-02-01

    Full Text Available Bromus sterilis L. (barren brome has become a troublesome weed of winter cereals in reduced tillage systems, mainly in South and North America, middle and Western Europe. In the Czech Republic, its importance has increased dramatically over the past 10 years. Barren brome is reported as a problem weed in other winter crops such as oil seed rape, in vineyards and in other cultivated places. In this study, the dormancy and germination under different temperatures, water and light regimes were investigated. Emergence from different depths and persistence in the soil profile were investigated under field conditions. The seeds of Bromus sterilis showed broad ecological valence to hydrothermal factors germinating in the wide range of 5 to 35 °C. Similarly, no strong effect on the germination in an environment with low water potential was observed. The response to light at various temperatures showed that seeds germinated better in darkness. The emergence declined significantly with burial depth (under 40 mm. The loss of primary dormancy was rapid in time and only 50% of the seeds germinated within 8 weeks after collecting from maternal plants. The seeds were not able to survive in the soil seed bank for a longer time and fall seeds lost viability 1 year after burial in a soil profile.

  10. The oxygen requirement of germinating flax seeds

    Kuznetsov, O.; Hasenstein, K.

    Experiments for earth orbit are typically prepared on the ground and often germinated in orbit in order to study gravity effects on developing seedlings. Germination requires the breakdown of storage compounds and respiration. In orbit the formation of a water layer around the seed may further limit oxygen availability. Therefore, the oxygen content of the available gas volume is one of the limiting factors for seed germination. In preparation for an upcoming shuttle experiment (MICRO on STS-107) we studied germination and growth of flax (Linum usitatissimum L.) seedlings in the developed hardware. We tested per seed chamber (gas volume = 14 mL, O2 = 2.9 mL) between 4 to 32 seeds glued to germination paper by 1% (w/v) gum guar. A lexan cover and a gasket hermetically sealed each of the eight chambers. For imbibition of the seeds a previously optimized amount of distilled water was dispensed through sealed inlets. The seedlings were allowed to grow for either 32 to 48 h on a clinostat or without microgravity simulation. Then their root length was measured. With 32 seeds per chamber, four times the intended number of seeds for the flight, the germination rate decreased from 94 to 69%, and the root length was reduced by 20%. Experiments on the germination and root length in controlled atmospheres (5, 10, 15 and 21% O2 ) suggest that germination and growth for two days requires about 200 :l of O (1 mL air) per seed. Our2 experiments correlate oxygen dependency from seed mass and germination temperature, and analyze accumulation of gaseous metabolites (supported by NASA grant NAG10-0190).

  11. Early planting and hand sorting effectively controls seed-borne fungi in farm-retained bean seed

    Ernest, Dube; Julia, Sibiya; Morris, Fanadzo.

    2014-12-01

    Full Text Available Home-saved bean (Phaseolus vulgaris L.) seed can be hand-sorted to remove discoloured seed, thereby reducing the level of contamination by certain seed-borne fungi and improving seed germination. In this study, the effect of planting date on the infection and discolouration of bean seed by seed-born [...] e fungi was investigated in order to improve the quality of hand-sorted, farm-retained bean seeds used by resource poor smallholder farmers. The germination quality and level of seed-borne fungi in hand-sorted first-generation bean seed harvested from an early-, mid- and late-summer season planted crop was therefore assessed. The highest percentage of discoloured seed (68%) was obtained from the mid-summer season planting. Non-discoloured seed from early- and late-season plantings had significantly (o

  12. Dormancy and growth vigour of seed potatoes.

    Van Ittersum, M.K.

    1992-01-01

    Dormancy is an important property of seed potatoes. Seed tubers planted too soon after their harvest do not produce plants because of dormancy, or produce low yields because of poor growth vigour. Potato tubers from the same cultivar vary in their duration of dormancy. The first aim of the research reported in this thesis was to predict the end of seed tuber dormancy, and to explain the variation in the duration of dormancy. The second aim was to investigate ways of curtailing the dormancy an...

  13. EPR in characterization of seeds paramagnetic species

    Luiz, A.P.C.; Mauro, M.F.F.L.; Portugal, K.O.; Barbana, V.M.; Guedes, C.L.B.; Mauro, E. di; Carneiro, C.E.A.; Zaia, D.A.M.; Prete, C.E.C. [Universidade Estadual de Londrina (UEL), PR (Brazil)

    2011-07-01

    Full text. In Brazil, since 1970s, renewable fuel programs has been developed in order to replace petroleum. Today a program that has been discussed is the bio diesel, which intend to replace diesel fuel, fossil oil, to bio diesel, renewal fuel. As seeds are the basis for production of oil and consequently processed into bio diesel, the goal of this work is to characterize and compare paramagnetic species present in the seeds by Electron Paramagnetic Resonance (EPR). Samples used in this study were seeds of sorghum, barley, corn, peanuts, soy beans, cotton, wheat, oats, mustard, rice, sunflower and turnip. Some paramagnetic species present in soil was also investigated as goethite (FeOOH), hematite (Fe{sub 2}O{sub 3}), magnetite (Fe{sub 3}O{sub 4}), and ferrihydrite (Fe{sub 5}HO{sub 8} {center_dot} 4H{sub 2}O), since, these species present in appreciable quantities in the soil can be present in the seeds and analyzed for comparison. The characterization of these species is essential to understand the EPR seeds spectra. Each sample is placed in a thin quartz tube 4 mm in diameter, and it is inserted into the cavity of the spectrometer at room temperature, at low temperature (77 K) and variable temperature using liquid nitrogen flow and hot flow through a compressor air. It was used as standard Mg O:Mn{sup 2+}, which is also inserted into the cavity. Shortly after the potency is regulated, frequency, amplitude and sweep the field. The spectroscopic analysis by EPR X-band ({approx} 9:5GHz), were performed at the Fluorescence and Electron Paramagnetic Resonance Laboratory, Exact Sciences Center, State University of Londrina, Parana state, Brazil, through an EPR spectrometer JEOL brand (JES-PE-3X). In the EPR spectra, spectroscopic factor or g factor and line width were determined in paramagnetic species. Studies from several seeds with EPR technique detected in all of them presence of same complex of Fe{sup 3+} present in the goethite at g {approx} 2, and in the seeds exist free radicals at g = 2:004, at room temperature. Ranging the temperature from 298 K until 77 K, the line width broadens, the cubic symmetry (g {approx} 2) disappears, and orthorhombic (g {approx} 9) and axial (g {approx} 4) symmetry appears in seeds spectra as well as in goethite spectra. Then, these results ratify the presence of goethite in all the seeds analyzed. Moreover, both the seed peel as the seed core has goethite, indicating that it is not a soil contamination, but actually goethite is absorbed by the seeds

  14. EPR in characterization of seeds paramagnetic species

    Full text. In Brazil, since 1970s, renewable fuel programs has been developed in order to replace petroleum. Today a program that has been discussed is the bio diesel, which intend to replace diesel fuel, fossil oil, to bio diesel, renewal fuel. As seeds are the basis for production of oil and consequently processed into bio diesel, the goal of this work is to characterize and compare paramagnetic species present in the seeds by Electron Paramagnetic Resonance (EPR). Samples used in this study were seeds of sorghum, barley, corn, peanuts, soy beans, cotton, wheat, oats, mustard, rice, sunflower and turnip. Some paramagnetic species present in soil was also investigated as goethite (FeOOH), hematite (Fe2O3), magnetite (Fe3O4), and ferrihydrite (Fe5HO8 · 4H2O), since, these species present in appreciable quantities in the soil can be present in the seeds and analyzed for comparison. The characterization of these species is essential to understand the EPR seeds spectra. Each sample is placed in a thin quartz tube 4 mm in diameter, and it is inserted into the cavity of the spectrometer at room temperature, at low temperature (77 K) and variable temperature using liquid nitrogen flow and hot flow through a compressor air. It was used as standard Mg O:Mn2+, which is also inserted into the cavity. Shortly after the potency is regulated, frequency, amplitude and sweep the field. The spectroscopic analysis by EPR X-band (∼ 9:5GHz), were performed at the Fluorescence and Electron Paramagnetic Resonance Laboratory, Exact Sciences Center, State University of Londrina, Parana state, Brazil, through an EPR spectrometer JEOL brand (JES-PE-3X). In the EPR spectra, spectroscopic factor or g factor and line width were determined in paramagnetic species. Studies from several seeds with EPR technique detected in all of them presence of same complex of Fe3+ present in the goethite at g ∼ 2, and in the seeds exist free radicals at g = 2:004, at room temperature. Ranging the temperature from 298 K until 77 K, the line width broadens, the cubic symmetry (g ∼ 2) disappears, and orthorhombic (g ∼ 9) and axial (g ∼ 4) symmetry appears in seeds spectra as well as in goethite spectra. Then, these results ratify the presence of goethite in all the seeds analyzed. Moreover, both the seed peel as the seed core has goethite, indicating that it is not a soil contamination, but actually goethite is absorbed by the seeds

  15. Improving Tree Seed Germination by Electrostatic Field

    Prof. Zhi-bin Gui

    2013-01-01

    Full Text Available Tree species with shallow dormancy are used for reforestation by airplane sowing in order to establish forest in mountain areas. To enhance germination, the quality of young seedling, an electrostatic field is used to treat pine seeds. Experiments found that treatment effect depended on the dosage, process and vigor index of seeds, and the optimal dosage was 500 kV/m 10 minutes for dry or wet seeds to improve germination, seedling height and root length during initial germination and middle and later stages of seedling development.

  16. Functionality-driven fractionation of lupin seeds

    Berghout, J.A.M.

    2015-01-01

    Functionality-driven fractionation of lupin seeds The growth in the world population requires an increase in the production of protein-rich foods from plant-based materials. Lupin seeds have potential to become a novel plant protein source for food products because they are rich in protein (about 37 wt%) and they can be grown in moderate temperature climates as in north-western Europe. Besides a high protein content, lupin seeds are rich in dietary fibres (soluble and insoluble), contain abou...

  17. Pre-sowing irradiation of vegetable seeds

    Results are reported of trials with radiation stimulation of vegetable crops conducted under farm conditions in different regions. They were based on exact tests accomplished at the IGHB. Pre-sowing irradiation of seed increased the greenhouse yield of Extase tomato variety by 15%; it increased the yield of Triumph tomato variety under field conditions as well. The pepper variety Kourtovska Kapiya and eggplant variety Bulgarski 12, both grown from irradiated seed in open field produced 18% and 5.5% higher yields, respectively. Irradiation of vegetable seeds finds a large application in the farm practice. (author)

  18. TAMARIND SEEDS CARBON: PREPRARTION AND METHANE UPTAKE

    K. Munusamy; Rajesh S. Somani; Hari C. Bajaj

    2011-01-01

    Tamarind seeds carbon (TSC) from tamarind (Tamarindus indica) seeds, an agro-byproduct and waste that is available abundantly in the southern states of India, was prepared by chemical activation with KOH. The influence of tamarind seeds char to KOH weight ratio (1:1 to 1:4) and activation temperature (400 to 800 °C) were investigated. TSC having micro-pore volume as high as 1.0 cm3/g with surface area 2673 m2/g was obtained. TSC was characterized by scanning electron microscopy, powder X-ray ...

  19. A reusable iodine-125 seed ophthalmic applicator

    A reusable iodine-125 seed ophthalmic applicator has been manufactured to treat intraocular tumours. It is made up of a stainless steel cap which houses a perspex insert; the perspex insert is used to contain four I-125 seeds. This applicator will treat a tumour with a surface diameter of 10mm and when loaded with four seeds each of activity 150MBq a dose of 60Gy may be delivered to a depth of 5mm in the eye in 5 to 6 days. The resultant dose to the sclera is of the order of 220Gy

  20. Chemical solution seed layer for rabits tapes

    Goyal, Amit; Paranthaman, Mariappan; Wee, Sung-Hun

    2014-06-10

    A method for making a superconducting article includes the steps of providing a biaxially textured substrate. A seed layer is then deposited. The seed layer includes a double perovskite of the formula A.sub.2B'B''O.sub.6, where A is rare earth or alkaline earth metal and B' and B'' are different rare earth or transition metal cations. A superconductor layer is grown epitaxially such that the superconductor layer is supported by the seed layer.

  1. Electrophoretic studies on rape seed proteins

    Electrophoresis is a technique which separates biological molecules on the basis of charge and mass properties. The technique is used for separation, purification, characterization and identification of molecules/ compounds. Two major objectives for applications of electrophoresis have been studied in this report i.e. characterization of rape seed proteins and enzymes and identification of rape seed cultivars by polyacrylamide gel electrophoresis (PAGE). Gamma irradiation is being successfully used to create genetic variability and germination which brought about definite changes in the rape seed proteins reflected in different bands. These differences could be used to study variability in crop plants. (A.B.)

  2. Protein determination in seeds by proton activation

    Morales, J.R.; Dinator, M.I. (Chile Univ., Santiago (Chile). Lab. de Fisica); Cerda, P. (Bio-Bio Univ., Chillan (Chile))

    1989-04-01

    A proton beam of 6.6 MeV has been used to produce /sup 11/C and /sup 13/N in Araucaria Araucana seeds. Their positron decay allows determination of the N/C ratio. In seeds the nitrogen content is associated to proteins while carbon is spread in the organic material. Samples were irradiated for about 10 min with a beam intensity of 5 nA on areas of 1 mm/sup 2/. Slices of the seed were radially explored, showing a larger concentration of protein in the center. (orig.).

  3. Protein determination in seeds by proton activation

    Morales, J. R.; Dinator, M. I.; Cerda, P.

    1989-04-01

    A proton beam of 6.6 MeV has been used to produce 11C and 13N in Araucaria Araucana seeds. Their positron decay allows determination of the N/C ratio. In seeds the nitrogen content is associated to proteins while carbon is spread in the organic material. Samples were irradiated for about 10 min with a beam intensity of 5 nA on areas of 1 mm 2. Slices of the seed were radially explored, showing a larger concentration of protein in the center.

  4. Protein determination in seeds by proton activation

    A proton beam of 6.6 MeV has been used to produce 11C and 13N in Araucaria Araucana seeds. Their positron decay allows determination of the N/C ratio. In seeds the nitrogen content is associated to proteins while carbon is spread in the organic material. Samples were irradiated for about 10 min with a beam intensity of 5 nA on areas of 1 mm2. Slices of the seed were radially explored, showing a larger concentration of protein in the center. (orig.)

  5. Conservação e qualidade pós-colheita de quiabo sob diferentes temperaturas e formas de armazenamento Postharvest conservation and quality of okra submitted to different temperatures and storage forms

    Wagner F da Mota

    2010-03-01

    Full Text Available O trabalho teve como objetivo avaliar a influência do filme plástico de PVC e da temperatura de armazenamento na conservação pós-colheita de frutos de cultivares de quiabo. O experimento foi organizado em delineamento de blocos casualizados, em esquema de parcelas subsubdivididas, tendo nas parcelas as duas temperaturas (5 e 10 ºC, nas subparcelas um fatorial 2 (sem e com PVC x 4 (cultivares Amarelinho, Red Velvet, Star of David e Mammoth Spinless e nas subsubparcelas os seis períodos de amostragem, com quatro blocos. A temperatura de 10ºC e o uso do filme de PVC foram mais eficientes no controle da perda de massa da matéria fresca. O filme foi mais eficiente na manutenção de maior teor relativo de água no pericarpo dos frutos armazenados a 5 e 10ºC. A cv. Amarelinho perdeu menos massa da matéria fresca e manteve maior teor relativo de água. Os frutos armazenados a 5ºC apresentaram maiores teores de vitamina C, com menor perda de vitamina C nas cvs. Mammoth Spinless e Star of David. Observou-se maior incidência de injúria por frio e escurecimento nos frutos armazenados sem PVC e a 5ºC. A cv. Amarelinho apresentou melhor conservação pós-colheita com temperatura de 10ºC e o uso da embalagem de PVC.This work had the goal to evaluate the influence of PVC film and temperature on the postharvest storage life in four cultivars of okra. The treatments were displayed in randomized complete blocks, in split-split-plot design, where the parcels were the temperatures of 5 and 10ºC, and in the sub parcels a 2 (control and PVC wrapped fruits x 4 (cultivars Amarelinho, Red Velvet, Star of David and Mammoth Spinless factorial, and in the sub-sub parcels six sampling moments, containing four blocks. Storage at 10ºC and wrapping the fruits with PVC film improved the control of fresh mass loss. The film was more efficient in maintaining higher water content in the fruit pericarp at 5 or 10ºC. The cultivar Amarelinho lost less fresh mass and maintained higher water content. Fruits stored at 5ºC had higher vitamin C content. The cultivars Mammoth Spinless and Star of David showed lower losses of vitamin C. Cultivar Mammoth Spinless had the highest content of chlorophyll and Amarelinho the lowest. In general the development of chilling and browning was higher in fruits without PVC film at 5ºC. The cultivar Amarelinho had better postharvest conservation at 10ºC and using PVC film.

  6. Armazenamento de frutos de quiabo embalados com filme de PVC em condição ambiente Shelf life of four cultivars of okra covered with PVC film at room temperature

    Wagner F da Mota

    2006-06-01

    Full Text Available O presente trabalho objetivou avaliar a influência do filme de PVC durante o armazenamento, em condição ambiente, de frutos de quatro cultivares de quiabo para consumo in natura. O experimento foi organizado segundo delineamento experimental em blocos casualizados, em esquema de parcelas subdivididas, tendo nas parcelas um fatorial 2x4, ou seja, embalagens sem e com PVC e quatro cultivares (Amarelinho, Red Velvet, Star of David, Mammoth Spinless, e nas subparcelas os seis períodos de amostragem com quatro blocos. As características avaliadas foram: massa fresca, teor relativo de água, teor de vitamina C, teor de clorofila total e análise visual do escurecimento. O uso do PVC proporcionou menor perda de massa fresca e maior teor relativo de água ao longo do armazenamento para todas as cultivares estudadas em relação às não embaladas com o filme. As menores perdas de massa fresca foram verificadas nas cvs. Amarelinho e Star of David sem e com PVC, respectivamente. Os frutos das cvs. Red Velvet e Star of David, embalados com PVC, apresentaram menores perdas no teor de vitamina C. Foi observado que a cv. Mammoth Spinless manteve maior teor de clorofila. Observou-se, de maneira geral, maior incidência de escurecimento nos frutos armazenados sem PVC. Verificou-se que de toda as cultivares estudadas o que manifestou melhor conservação com relação ao escurecimento foi o Red Velvet.The present work evaluated the influence of PVC film on the postharvest shelf life of four cultivars of okra stored at room temperature. The experiment was arranged in random blocks, in sub split parcels, where the parcels were a factorial 2x4, with and without PVC film and the cvs. Amarelinho, Red Velvet, Star of David and Mammoth Spinless, and the subparcels the six sample time with four blocks. The characteristics evaluated were: percentage of weight loss, relative water content, total chlorophyll and chlorophyll a and b, content of vitamin C, visual browning. The PVC was efficient in controlling the weight loss and retained the water content through storage. The lowest losses were observed for the cvs. Amarelinho and Star of David, without and with PVC, respectively. There was higher retention of vitamin C in the fruits wrapped with PVC and the cvs. Red Velvet and Star of David kept higher content of it. In general, the cv. Mammoth Spinless kept higher content of chlorophyll. The higher incidence of browning was in fruits stored without PVC. The Red Velvet had better shelf life due to lower browning.

  7. Seed size variation in the palm Euterpe edulis and the effects of seed predators on germination and seedling survival

    Pizo, Marco A.; Von Allmen, Christiane; Morellato, L. Patricia C.

    2006-05-01

    Intraspecific variation in seed size is common in wild plant populations and has important consequences for the reproductive success of individual plants. Multiple, often conflicting evolutionary forces mediated by biotic as well as abiotic agents may maintain such a variation. In this paper we assessed seed size variation in a population of the threatened, commercially important palm Euterpe edulis in southeast Brazil. We investigated (i) how this variation affects the probability of attack by vertebrate and invertebrate post-dispersal seed predators, and (ii) if seed size influences the outcome of seeds damaged by beetles in terms of seed germination and early survival of seedlings. Euterpe edulis seeds varied in diameter from 8.3 to 14.1 mm. Neither insects nor rodents selected the seeds they preyed upon based on seed size. Seed germination and total, shoot and root biomasses of one-year seedlings were significantly and positively affected by seed size. Root biomass and seedling survival were negatively affected by seed damage caused by a scolytid beetle ( Coccotrypes palmarum) whose adults bore into seeds to consume part of the endosperm, but do not oviposit on them. Seed size had a marginally significant effect on seedling survival. Therefore, if any advantage is accrued by E. edulis individuals producing large seeds, this is because of greater seed germination success and seedling vigor. If this is so, even a relatively narrow range of variation in seed size as observed in the E. edulis population studied may translate into differential success of individual plants.

  8. Seed germination and seedling fitness in Mesua ferrea L. in relation to fruit size and seed number per fruit

    Khan, M. Latif; Bhuyan, Putul; Shankar, Uma; Todaria, Nagendra P.

    1999-11-01

    Effect of fruit size and seediness (seed number per fruit) was examined on germination and early growth of seedlings in Mesua ferrea L. Fruiting incidence (number of fruited trees in a population) and fruit loading (number of fruits per tree) vary from one year to the other, and were greater in 1997 than in 1998. Seeds from large fruits (> 40 g) are preferred for forestry plantations and those from small fruits (germination percentage of seeds increased from 1-seeded through 4-seeded fruits both in laboratory and greenhouse conditions, and both in case of large and small fruits. Conversely, the mean seed weight and germination time decreased along this gradient, i.e. seeds from 1-seeded fruits were the heaviest and required maximum time for germination, and the seeds from 4-seeded fruits were the lightest and required minimum time for germination. The seeds from small fruits were lighter in weight, achieved lower germination percentages and required greater germination time than the seeds from large fruits in all four seeded categories. Seedlings from seeds from 1-seeded fruits survived better and with stronger vigour after 1 year of growth than seedlings from 2-, 3- and 4-seeded fruits. Further, seedling survival and vigour were greater for seeds from large rather than small fruits.

  9. Influence of Rice Seeding Rate on Efficacies of Neonicotinoid and Anthranilic Diamide Seed Treatments against Rice Water Weevil

    Jason Hamm

    2014-12-01

    Full Text Available Rice in the U.S. is frequently seeded at low rates and treated before sowing with neonicotinoid or anthranilic diamide insecticides to target the rice water weevil. A previous study of the influence of seeding rate on rice water weevil densities showed an inverse relationship between seeding rates and immature weevil densities. This study investigated interactive effects of seeding rate and seed treatment on weevil densities and rice yields; in particular, experiments were designed to determine whether seed treatments were less effective at low seeding rates. Four experiments were conducted over three years by varying seeding rates of rice treated at constant per seed rates of insecticide. Larval suppression by chlorantraniliprole was superior to thiamethoxam or clothianidin, and infestations at low seeding rates were up to 47% higher than at high seeding rates. Little evidence was found for the hypothesis that seed treatments are less effective at low seeding rates; in only one of four experiments was the reduction in weevil densities by thiamethoxam greater at high than at low seeding rates. However, suppression of larvae by neonicotinoid seed treatments in plots seeded at low rates was generally poor, and caution must be exercised when using the neonicotioids at low seeding rates.

  10. Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

    Agnieszka I. Piotrowicz-Cie?lak

    2011-04-01

    Full Text Available Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L. cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC. Seed storage at room temperature reduced viability (to 2% and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered.

  11. Effect of Seeding Date on Seed Yield and Yield Components in Edible- oil Genotypes of Flax in Isfahan

    G. Saeidi

    2002-10-01

    Full Text Available Edible-oil flax (Linum usitatissimum L. can be an alternate oil-seed crop in Isfahan region. Seeding date is very important in obtaining higher grain yields and a successful flax production. The present study was conducted at the research farm, Isfahan University of Technology in the year 2000 to determine the effect of seeding date and genotype on yield, yield components and maturity of edible-oil flax. In this study, a RCBD with three replications, in which the treatments were organized as a split-plot experiment, was used. Seeding dates (Oct. 17, Nov. 16, March 15, April 13, May 14, June 13, July 15 and genotypes (four breeding lines of edible-oil flax were considered as the main and the subfactor, respectively. The number of capsules per plant, seeds per capsule, 100-seed weight and seed yield were highest for all genotypes in the first seeding date. Average seed yield in the first seeding date was almost twice, triple and eight times as much as the second, third and the last three seeding dates, respectively. In the second seeding date there was no emergence because of low temperature. The genotype and seeding date interaction on seed yield and maturity was significant. In general, delayed seeding was accompanied by reduction of emergence, number of days to maturity, yield and yield components in all genotypes, with the exception that the last seeding date led to increased number of days to maturity and seeds per capsule. Based on regression analysis, seed weight, number of seeds per capsule and number of seedlings/m2 were the most important components that contributed in seed yield variation, respectively. But, most of the variations in seed yield per plant was affected by number of copsules per plant and number of seeds per capsule, and in that order.

  12. Seed dimorphism, nutrients and salinity differentially affect seed traits of the desert halophyte Suaeda aralocaspica via multiple maternal effects

    Wang Lei

    2012-09-01

    Full Text Available Abstract Background Maternal effects may influence a range of seed traits simultaneously and are likely to be context-dependent. Disentangling the interactions of plant phenotype and growth environment on various seed traits is important for understanding regeneration and establishment of species in natural environments. Here, we used the seed-dimorphic plant Suaeda aralocaspica to test the hypothesis that seed traits are regulated by multiple maternal effects. Results Plants grown from brown seeds had a higher brown:black seed ratio than plants from black seeds, and germination percentage of brown seeds was higher than that of black seeds under all conditions tested. However, the coefficient of variation (CV for size of black seeds was higher than that of brown seeds. Seeds had the smallest CV at low nutrient and high salinity for plants from brown seeds and at low nutrient and low salinity for plants from black seeds. Low levels of nutrients increased size and germinability of black seeds but did not change the seed morph ratio or size and germinability of brown seeds. High levels of salinity decreased seed size but did not change the seed morph ratio. Seeds from high-salinity maternal plants had a higher germination percentage regardless of level of germination salinity. Conclusions Our study supports the multiple maternal effects hypothesis. Seed dimorphism, nutrient and salinity interacted in determining a range of seed traits of S. aralocaspica via bet-hedging and anticipatory maternal effects. This study highlights the importance of examining different maternal factors and various offspring traits in studies that estimate maternal effects on regeneration.

  13. Secondary dispersal of bigcone Douglas-fir ( Pseudotsuga macrocarpa ) seeds

    Vander Wall, Stephen B.; Borchert, Mark I.; Gworek, Jennifer R.

    2006-07-01

    Large-seeded pines ( Pinus spp.) are known to be dispersed by seed-caching corvids (i.e. jays and nutcrackers) and rodents (e.g. chipmunks and mice), with a concomitant decrease in seed dispersability by wind. We tested the idea that seeds of bigcone Douglas-fir ( Pseudotsuga macrocarpa), which are winged but larger than the seeds of other members of Pseudotsuga, are dispersed by a combination of wind and seed-caching rodents. We compared characteristics of seeds from P. macrocarpa in southern California (mean seed mass 132.6 mg) to seeds of a population of Pseudotsuga menziesii (Douglas-fir) in northern California (24.8 mg). We also tested whether rodents would cache seeds of P. macrocarpa. Seeds of P. macrocarpa had greater wing loadings (1.37 mg/mm 2) and descent velocities (2.47 m/s) than those of P. menziesii (0.52 mg/mm 2 and 1.28 m/s, respectively). These data indicate that the wind dispersability of P. macrocarpa is likely to be less than that of P. menziesii, but this loss of wind dispersability is partially compensated for by secondary dispersal of seeds by rodents, which readily gathered and cached the larger seeds of P. macrocarpa up to 34 m from source trees. Large seed size confers several advantages to P. macrocarpa, most importantly attracting seed-caching animals that effectively bury seeds.

  14. Radish (Raphanus sativus) seed size affects germination response to coumarin

    The inhibition of seed germination by an allelochemical is generally greater in small seeds than in large seeds. Studies reporting these results used a large number of plant species that varied in seed size, which might have introduced differences in germination characteristics or various parameter...

  15. Phytotoxicity of mustard seed meals alone and in combinations

    Mustard seed meal is produced when oil is extracted from brassicaceous seeds. The high glucosinolate content of these seed meals makes them of interest as management agents for weeds and soilborne pathogens. Previous studies indicated that seed meals from Brassica juncea and Sinapis alba are nemat...

  16. 7 CFR 97.143 - Certified seed only.

    2010-01-01

    ... only as a class of certified seed, the owner, or his or her designee, may label containers of seed of the variety substantially as follows: “Unauthorized Propagation Prohibited—U.S. Variety Protection... variety name only, as a class of certified seed, may label containers of the seed of the...

  17. 7 CFR 201.49 - Other crop seed.

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Other crop seed. 201.49 Section 201.49 Agriculture... REGULATIONS Purity Analysis in the Administration of the Act § 201.49 Other crop seed. (a) Seeds of plants grown as crops (other than the kind(s) and variety(ies) included in the pure seed) shall be...

  18. Seed longevity and germination characteristics of six fen plant species.

    Tatár, S

    2010-01-01

    Fens are among the most threatened habitats in Europe as their area has decreased considerably in the last centuries. For successful management and restoration conservationists need detailed knowledge about seed bank formation and seed longevity of plants, as these features are closely related to successional and vegetation dynamical processes. I analysed seed longevity and the germination characteristics of six fen plant species by seed burial experiments. Based on seed weight, seed bank was expected for long-term persistent for the light-seeded Schoenus nigricans, Carex appropinquata, C. pseudocyperus, C. davalliana and Peucedanum palustre and also that for the medium-seeded Cicuta virosa. It was proved that, the latter two species have short-term persistent seed banks, while Carex pseudocyperus has a transient seed bank, therefore these species may only have a limited role in restoration from seed banks. It was found that Schoenus nigricans, Carex appropinquata and C. davalliana have persistent seed banks, because some of their four-year-old seeds have emerged. Fresh seeds had low germination rate in all studied species and majority of seeds emerged after winter, except for Carex pseudocyperus. After the germination peak in spring, the majority of the ungerminated seeds of Schoenus nigricans, Peucedanum palustre, Carex appropinquata, C. davalliana and Cicuta virosa entered a secondary dormancy phase that was broken in autumn. I found the seasonal emergence of the latter three species highly similar. PMID:21565777

  19. Seed potato production system in Japan, starting from foundation seed of potato

    Kawakami, Tsukasa; Oohori, Hidemiki; Tajima, Kazuyuki

    2015-01-01

    Potato is one of the staple crops cultivated in upland farming in Japan and is propagated vegetatively by means of tubers. However once infected with diseases, potato yield decreases significantly. And one seed potato can produce approximately only 10 potato tubers. To improve the production system of seed potatoes in Japan, Japanese government established a three-stage propagation system for the production and distribution of healthy and disease-free seed potatoes. The National Center for Se...

  20. Germination in seed species ingested by opossums: implications for seed dispersal and forest conservation

    Nilton Carlos Cáceres; Emygdio Leite de Araújo Monteiro-Filho

    2007-01-01

    Seed germination in plant species consumed by opossums, genus Didelphis, was investigated in southern Brazil, in order to improve knowledge of the strategies of zoochorous plants in the Neotropics. Seeds were obtained from opossum feces. Thirteen of the most frequent species in the diet of local opossums were tested for germination rates and germination responses under different qualities (red/far red ratio) and different intensities of light. Most seeds from feces germinated similarly to the...

  1. ANTIFUNGAL ACTIVITY ASSOCIATED WITH Psoralea corylifolia Linn. (BAKUCHI) SEED AND CHEMICAL PROFILE CRUDE METHANOL SEED EXTRACT

    A. BORATE; M. UDGIRE; A. KHAMBHAPATI

    2014-01-01

    Objective: Present study aims to evaluate antifungal efficacy of Bakuchi (Psoralea corylifolia) seed extracts prepared in methanol solvents and the bakuchi oil. Bakuchi seed used in the formulations against skin related diseases and disorders in Ayurvedic system of medicine. Method: Antifungal assay was performed by agar well diffusion method against common fungal skin pathogens Candida albicans, Aspergillus niger and Malassezia furfur. Results: Bakuchi seeds extract in methanol was obs...

  2. IMPORTANCE OF STORAGE CONDITIONS AND SEED TREATMENT FOR SUNFLOWER HYBRIDS SEEDS GERMINATION

    Goran Krizmanić

    2014-12-01

    Full Text Available In this research we have determined germination energy and germination of seeds of sunflower hybrids ‘Luka’ and ‘Apolon’, at the beginning of storage and 6, 12 and 18 months after of storage period (2011-2012 in the floor concrete storage at two different air temperatures and humidity (S-1: air temperature 15-18°C and relative air humidity 65-70% as well as in climate chamber (S-2: air temperature 10-12°C and relative air humidity 60-65%, stored in four treatments (Control: processed-untreated seed; T-1: treated with A.I. metalaxyl-M; T-2: treated with A.I. metalaxyl-M + A.I. imidacloprid and T-3: treated with A.I. metalaxyl-M + A.I. clothianidin. Based on the obtained results we have determined that sunflower hybrid ‘Luka’, compared to hybrid ‘Apolon’, in the given storage conditions and with the same seed treatment has 5-8% higher germination energy and seed germination and that in climate chamber both hybrids have 5-7% higher germination energy. Seed treatment of both sunflower hybrids with A.I. imidacloprid maximally reduced initial germination energy and seed germination in all tested periods and conditions of storage. On the average, natural seed, after 18 months of storage did not have better seed quality compared to seed treated with A.I. metalaxyl-M while other treatments had more significant influence on reduction of germination energy and seed germination, 6-15%. On the average, compared to other variants, seeds treated with A.I. metalaxyl-M after 18 months of storage in both storage conditions had higher germination energy by 4-15%, and seed germination by 2-12%.

  3. ROS Signaling in Seed Dormancy Alleviation.

    Bouteau, Hayat El-Maarouf; Job, Claudette; Job, Dominique; Corbineau, Françoise; Bailly, Christophe

    2007-09-01

    Reactive oxygen species have been suggested to play a signaling role in seed dormancy alleviation. When sunflower seeds become able to fully germinate during dry after-ripening, they accumulate high amount of hydrogen peroxide and exhibit a low detoxifying ability through catalase, resulting from the decrease in CATA1 transcript. ROS accumulation entails oxidative modification of soluble and storage proteins through carbonylation, which suggests that this process might play an important role in plant developmental processes. However other oxidative signaling pathways cannot be excluded. For example, a cDNA-AFLP study shows that seed after-ripening is also associated with changes in gene expression and that changes in ROS content during seed imbibition are also related to changes in expression pattern. PMID:19704599

  4. Hypolipidemic effects of fenugreek seed powder

    Abu Saleh M. Moosa, Mamun Ur Rashid, A.Z.S. Asadi, Nazma Ara, M. Mojib Uddin and A. Ferdaus

    2006-01-01

    Effects of fenugreek (Trigonella foenugraecum Linn) on serum lipid profile in hypercholesteremic type 2 diabetic patients were studied. Administration of fenugreek seed powder of 25 gm orally twice daily for 3 weeks and 6 weeks produces significant (P

  5. Prairie remnant seed collection data, 1992

    US Fish and Wildlife Service, Department of the Interior — This is a record of prairie seed collections on Neal Smith National Wildlife Refuge formerly Walnut Creek National Wildlife Refuge from 1992.

  6. Shaping metal nanocrystals through epitaxial seeded growth

    Habas, Susan E.; Lee, Hyunjoo; Radmilovic, Velimir; Somorjai,Gabor A.; Yang, Peidong

    2008-02-17

    Morphological control of nanocrystals has becomeincreasingly important, as many of their physical and chemical propertiesare highly shape-dependent. Nanocrystal shape control for both single andmultiple material systems, however, remains fairly empirical andchallenging. New methods need to be explored for the rational syntheticdesign of heterostructures with controlled morphology. Overgrowth of adifferent material on well-faceted seeds, for example, allows for the useof the defined seed morphology to control nucleation and growth of thesecondary structure. Here, we have used highly faceted cubic Pt seeds todirect the epitaxial overgrowth of a secondary metal. We demonstrate thisconcept with lattice matched Pd to produce conformal shape-controlledcore-shell particles, and then extend it to lattice mismatched Au to giveanisotropic growth. Seeding with faceted nanocrystals may havesignificant potential towards the development of shape-controlledheterostructures with defined interfaces.

  7. Effects of gamma radiation in tomato seeds

    Wiendl, Toni A.; Wiendl, Fritz W.; Franco, Suely S.H.; Franco, Jose G.; Althur, Valter, E-mail: tawiendl@hotmail.com, E-mail: gilmita@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Arthur, Paula B., E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2013-07-01

    Tomato dry seeds of the hybrid 'Gladiador' F1 were exposed to low doses of gamma radiation from Co-60 source at 0,509 kGy tax rate in order to study stimulation effects of radiation on germination and plant growth. Eight treatments radiation doses were applied as follows: 0 (control); 2,5; 5,0; 7,5; 10,0; 12,5; 15,0; 20,0 Gy. Seed germination as well as green fruits number, harvested fruit number, fruit weight and total production were assessed to identify occurrence of stimulation. Tomato seeds and plants were handled as for usual tomato production in Brazil. Low doses of gamma radiation treatment in the seeds stimulate germination and substantially increase fruit number and total production up to 86% at 10 Gy dose. There are evidences that the use of low doses of gamma radiation can stimulate germination and plant production thus, showing hormetic effects. (author)

  8. KNOW MORE ABOUT PROPITIOUS SEED-FLAX

    Sirisha M*

    2010-06-01

    Full Text Available Linseed, linum usitatissimum is used in Ayuveda for many Vata, Pitta related disorders. The lipid content of the seed contains more than 55% of omega – 3 fatty acid responsible for hypolipidaemic,anticancer, anti inflammatory, anti burn, anti rheumatic, antithrombic and proven useful in atherosclerosis, arrhythmias, cardiac disorders, also in autoimmune diseases. Lignan content contains cyanogenic glucosides and phyto oestrogens. The whole seed is very useful and is known from the ancient times for its activities.Recent years, again people has realized about the valuable therapeutic activities and immense research work already been started. The use of seed is recommended for its omega-3, omega-6 and omega-9, phytoestrogens and cyanogenic glucosides. All these ingredients with diverse pharmacotherapeutic activities make it a propitious seed.

  9. The Flying Sunflower: A Seed Dispersal Project.

    Buege, Douglas J.

    1999-01-01

    Describes an open-ended activity in which students build a "plant" that launches its seeds as far as possible to study the dispersal strategies of various plants. Recommends extension activities for elementary- and secondary-level students. (WRM)

  10. Compact 2 Micron Seed Laser Project

    National Aeronautics and Space Administration — This proposal is for the development of innovative compact, high power and extremely reliable 2 micron seed laser using newly developed Tm3+ doped germanate glass...

  11. Compact 2 Micron Seed Laser Project

    National Aeronautics and Space Administration — This proposal is for the development of new compact, high power and extremely reliable 2 micron seed laser using newly developed Tm3+ doped germanate glass fibers,...

  12. Seed radiosensitivity of a hexaploid triticale

    Seeds of the winter hexaploid triticale 6TA-131 containing approximately equal to 13% water were irradiated with either (230 to 1520 rads). Survival at three weeks and height at four weeks of field-grown plants were reduced significantly only at the highest doses of radiation. However, plants from seeds irradiated with 1520 rads of fission neutrons died later in the season. Mean percent seed set following enforced self-fertilization, an endpoint of significance to a mutation induction program, was reduced to approximately equal to 45% of control by either 46.50 krads of gamma radiation or 685 rads of fission neutrons. Weight per 1000 seeds was only slightly affected by radiation dose. (author)

  13. Seed train optimization for cell culture.

    Frahm, Björn

    2014-01-01

    For the production of biopharmaceuticals a seed train is required to generate an adequate number of cells for inoculation of the production bioreactor. This seed train is time- and cost-intensive but offers potential for optimization. A method and a protocol are described for the seed train mapping, directed modeling without major effort, and its optimization regarding selected optimization criteria such as optimal points in time for cell passaging. Furthermore, the method can also be applied for the set-up of a new seed train, for example for a new cell line. Although the chapter is directed towards suspension cell lines, the method is also generally applicable, e.g. for adherent cell lines. PMID:24297426

  14. Effects of gamma radiation in tomato seeds

    Tomato dry seeds of the hybrid 'Gladiador' F1 were exposed to low doses of gamma radiation from Co-60 source at 0,509 kGy tax rate in order to study stimulation effects of radiation on germination and plant growth. Eight treatments radiation doses were applied as follows: 0 (control); 2,5; 5,0; 7,5; 10,0; 12,5; 15,0; 20,0 Gy. Seed germination as well as green fruits number, harvested fruit number, fruit weight and total production were assessed to identify occurrence of stimulation. Tomato seeds and plants were handled as for usual tomato production in Brazil. Low doses of gamma radiation treatment in the seeds stimulate germination and substantially increase fruit number and total production up to 86% at 10 Gy dose. There are evidences that the use of low doses of gamma radiation can stimulate germination and plant production thus, showing hormetic effects. (author)

  15. Abscisic acid and assimilate partitioning during seed development.

    Bruijn, S. M.

    1993-01-01

    This thesis describes the influence of abscisic acid (ABA) on the transport of assimilates to seeds and the deposition of reserves in seeds. It is well-known from literature that ABA accumulates in seeds during development, and that ABA concentrations in seeds correlate rather well with seed size and seed growth rates. However, since ABA is at least partly synthesized in the leaves and transported to the seeds via the phloem, a correlation between ABA levels and growth rate can easily be expl...

  16. Recovery and Germination of Grass Seeds Ingested by Cattle

    Ferhat Gökbulak

    2006-01-01

    Seeds of bluebunch wheatgrass [Psuedoroegneria spicata (Pursh) A. Love] and Sandberg bluegrass (Poa secunda Presl.) were fed to Holstein heifers in different amounts to investigate the effects of seed feeding level and seed size on the recovery and germinability of passed seed. Animals were fed 60,000, 30,000,15,000, and 7,500 seeds of each species. Passed seeds were recovered from dungcollected daily over a 4-day period and tested for germinability. In general, recovery of the larger-seeded ...

  17. Factors affecting the density of Brassica napus seeds

    Young, L.; Jalink, H.; Denkert, R.; Reaney , M

    2006-01-01

    Brassica napus seed is composed of low density oil (0.92 g.cm(-3)) and higher density solids (1.3-1.45 g.cm(-3)). Seed buoyant density may potentially be used to determine seed oil content and to separate seeds with different oil contents, however, we have found that seeds with the lowest buoyant density had lower than expected oil contents. It is proposed that the low oil content observed in the lowest density seed is a function of air gaps or pockets within the seed coat with sufficient vol...

  18. Radiological response of lanthanum guiding seeds in brachytherapy implants

    Ceramic seeds with La-139 incorporated were synthesized to be used as radiological guides in brachytherapy implants. The synthesis was performed based on the sol-gel method. The seeds were subjected to characterization by Scanning Electron Microscopy, X-ray diffraction and Energy-Dispersive X-ray Spectroscopy. Furthermore, the contrast from a radiographic film was evaluated to lanthanum, samarium and holmium seeds. Radiological response on a phantom at different depths with lanthanum seeds and metal seeds was also investigated. Based on the values of contrast, the synthesized lanthanum seeds can be considered efficient as radiological guides when implanted together with pure Ho-165 and Sm-152 seeds. (author)

  19. Alkaline Seed-Bed: An Innovative Technique for Manifesting Verticillium dahliae on Fennel Seeds

    Khalid M. Ghoneem

    2009-01-01

    Full Text Available Verticillium dahliae attacks a wide range of plants including fennel causing a wilt disease. The fungus grows slowly on seeds when tested at the seed health laboratories. This habit character allows saprophytes to impair the fungal growth and interfere the identification on both Moist Blotters (MB and the Deep-Freezing Blotters (DFB. Since, these two techniques are not efficient enough to detect this fungus, the researchers planned to search for an alternative technique for detecting this fungus. Soaking three layers of blotters used as seed-beds in water solutions alkalined with KOH or NaOH at pH 10 presents the optimum seed-bed condition for manifesting the fungus on seed. This seed-bed condition also suppress the growth of saprophytes, so as the fungus was transparently shown on seeds. The in vitro study presents pH 9.5 as the optimum condition for the growth, sporulation and maximum glucose coefficient of the fungus. So far, it is recommended to use the alkalined seed-bed when searching for V. dahliae on fennel seed.

  20. Water Productivity of Irrigated Rice under Transplanting, Wet Seeding and Dry Seeding Methods of Cultivation

    Murali, NS.

    1997-01-01

    Full Text Available Water productivity (WP of irrigated lowland rice was determined during the 1994 dry (January to May and wet (August to December seasons on a heavy clay acid sulphate soil. Treatments consisted of three cultivation methods : transplanted rice, pregerminated seeds broadcasted on puddled soil (wet seeding and dry seeds broadcasted on unpuddled soil (dry seeding. In wet and dry seeded plots, continuous standing water condition was initiated 17 days after sowing. Total water requirement for rice production was highest in transplanted plots (755 mm in wet season and 1154 mm in dry season and was lowest in dry seeded plots (505 mm in wet season and 1040 mm in dry season. Dry seeding required no water for land preparation but transplanting and wet seeding methods required 18 - 20 % of total water requirement in dry season and 27 - 29 % in wet season. Total percolation was maximum (99 mm in wet season and 215 mm in dry season in dry seeding method and was minimum (62 mm in wet season and 94 mm in dry season in transplanting method. In dry and wet seeding methods, daily percolation gradually decreased with the age of the crop. Total seepage loss did not show any significant difference between the cultivation methods in the two seasons. Grain yield was not affected by the three cultivation methods in both seasons. Water productivity (the ratio between grain yield and total amount of water used in production was 3.5 - 4.1 kg ha-1 mm-1, 3.8 - 4.4 kg ha-1 mm-1 and 4.1 - 5.5 kg ha-1 mm-1 in transplanted, wet seeded and dry seeded rice, respectively. Labour requirement for land preparation and sowing was maximum in transplanted (219 - 226 man-hours ha-1 followed by wet (104 -112 man-hours ha-1 and dry seeded (94 - 99 man-hours ha-1 methods. However, in wet season extra labour (77 man-hours ha-1 was required for weeding after crop establishment in dry and wet seeding methods. Crop maturity was 20 days earlier in wet and dry seeding methods compared to transplanting. Dry seeding was considered the best rice cultivation method on heavy clay soils.