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British Library Electronic Table of Contents (United Kingdom)

Phage display is effective in screening peptides that mimic venom's neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected heptapeptide sequences were aligned with known protein sequences of NAV and BMV in GenBank. One of the four consensus sequences, L/PKSSLL, mimicked the crucial epitope on Loop III of Taiwan cobra cardiotoxin that is associated with the venom's lethal potency. In dot blot analysis, several clones showed varying reactivities for NAV monovalent antivenin and lesser cross-reactions with BMV monovalent antivenin. The KSSLLRN-carrying phage occurred four times in selected clones and showed the strongest ...

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How to build a digital library

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Digital Library Technology Trends

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ACM Digital Library [Online database

International Science & Technology Center (ISTC)

Natural Biologically Active HLDF6 Peptide as Potential Preparation for Correction of Different Drugabuse Forms

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The Design of an Interactive Online Help Desk in the Alexandria Digital Library

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Emerging technologies for academic libraries in the digital age

CERN Document Server

Artificial intelligence in digital libraries

J-STORE (Japan)

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Science.gov (United States)

... type. Sonic's digital libraries will provide a cross mapping between the narrative (speech and text) and imagery domains. ...

UK PubMed Central (United Kingdom)

A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was isolated....Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe binding of peptide fragments of antigens to class II MHC is a crucial step in initiating a helper T cell immune response. The identification of such peptide epitopes...Full Text Available

UK PubMed Central (United Kingdom)

The sequences of the peptide binding domains of 33 70 kd heat shock proteins (hsp70) have been aligned and a consensus secondary structure has been deduced. Individual members showed no significant...Full Text Available

Science.gov (United States)

The family of natriuretic peptides consists of the atria natriuretic peptide (ANP), the cerebral natriuretic peptide (BNP), the type C natriuretic peptide (CNP) and the peptide isolated from the dendroaspis snakes' poison (DNP), whose presence in humans has not been confirmed. The physiological function of ANP is in the control of arterial blood pressure by regulation of systemic vascular resistance of blood vessels. BNP is produced as one of the factors in the acute response to inflammatory tissue damage, mainly in coronary vessels. Increased serum concentrations of natriuretic peptides have been found in stress situations, such as trauma or major surgery, systemic hypotension, and in intrinsic myocardial dysfunction. High concentrations of natriuretic peptides were observed in severe sepsis, septic shock and in multiple organ failure, ...

International Nuclear Information System (INIS)

Selected neutron reaction nuclear data libraries and photon-atomic interaction cross section libraries for elements of interest to the IAEA's program on Fusion Evaluated Nuclear Data Library (FENDL) have been processed into MATXSR format using the NJOY system on the VAX4000 computer of the IAEA. This document lists the resulting multigroup data libraries. All the multigroup data generated are available cost-free upon request from the IAEA Nuclear Data Section. (author). 9 refs.

Science.gov (United States)

Four major natriuretic peptides have been isolated: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), C-type natriuretic peptide (CNP), and Dendroaspis-type natriuretic peptide (DNP). Natriuretic peptides play an important role in the regulation of cardiovascular homeostasis maintaining blood pressure and extracellular fluid volume. The classical endocrine effects of natriuretic peptides to modulate fluid and electrolyte balance and vascular smooth muscle tone are complemented by autocrine and paracrine actions that include regulation of coronary blood flow and, therefore, myocardial perfusion; modulation of proliferative responses during myocardial and vascular remodeling; and cytoprotective anti-ischemic effects. The actions of natriuretic peptides are mediated by the specific binding of these ...

KoreaScience (Korea)

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British Library Electronic Table of Contents (United Kingdom)

C-type natriuretic peptide (CNP) is a member of the small family of natriuretic peptides that also includes atrial natriuretic peptide (ANP) and brain, or B-type natriuretic peptide (BNP). Unlike them, it performs its major functions in an autocrine or paracrine manner. Those functions, mediated through binding to the membrane guanylyl cyclase natriuretic peptide receptor B (NPR-B), or by signaling through the non-enzyme natriuretic peptide receptor C (NPR-C), include the regulation of endochondral ossification, reproduction, nervous system development, and the maintenance of cardiovascular health. To date, the regulation of CNP gene expression has not received the attention that has been paid to regulation of the ANP and BNP genes. CNP expression in vitro is regulated by TGF-b and recepto...

Science.gov (United States)

Tutorial on digital libraries. Site also contains links to other reference materials on digital libraries as well as links to courses on the subject. Keywords: Information Systems - Information Systems Applications, Information Storage and Retrieval, Database Management, multimedia

UK PubMed Central (United Kingdom)

Objective: The paper describes and evaluates the use of Clinical Digital Libraries Project (CDLP) digital library collections in terms of their facilitation of timely clinical information...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Using data on libraries collected by the Association of Research Libraries (ARL) and the Association of College and Research Libraries (ACRL), as well as fall-to-fall retention rates obtained from the Integrated Postsecondary Education Data System (IPEDS) of the National Center for Education Statistics (NCES), this study employs statistical measures of association to analyze the relationship of both library expenditures and number of professional library staff to student persistence. The strongest relationships found were those between student retention and total library expenditures, total library materials costs, and serial costs for institutions categorized as baccalaureate colleges within the Carnegie Classification System. The most significant relationship between persistence and numb...

Science.gov (United States)

... Science, Computer Vision, Digital Government, Digital Libraries, Digital Society and Technologies ... for Robust Intelligence, Digital Government, and Digital Libraries and Archives. In subsequent years ...

J-STORE (Japan)

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CERN Document Server

The paper represents the result of research in the field of digital libraries functionalities in the context of new Grid infrastructure support. It is defined a new vector of knowledge society, informational vector - content vector. It presents a Grid European project which includes Romanian partners, and it defines on this base a digital library model which can be applied for the libraries in Romania.

Science.gov (United States)

PMID:19147296

International Nuclear Information System (INIS)

A multi-group neutron-gamma cross-section library in DTF-IV format is supplemented with electron production matrix to generate a coupled neutron-gamma-electron library. This is realized by estimating the contributions from Compton scattering, pair production and photoelectric effect to the electron production cross-sections. A novel application of this new library, which involves transport of neutrons, gammas and electrons, for estimating the Compton current due to a pulse of radiation in air is discussed.

UK PubMed Central (United Kingdom)

BackgroundCalcitonin gene related peptide (CGRP) is a neuropeptide that is abundant in the sensory neurons which innervate bone. The effects of CGRP on isolated bone cells have been...Full Text Available

UK PubMed Central (United Kingdom)

A unique antibacterial peptide derivative found in immune honeybee lymph, apidaecin 1b (AP1), was randomly mutagenized and characterized by a newly established system to analyze in vivo its structure-function...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Atrial natriuretic peptide (ANP) causes relaxation in the opossum lower esophageal sphincter. The effects of dendroaspis natriuretic peptide (DNP) and other natriuretic peptides in the lower esophageal sphincter were not known. We measured the relaxation of transverse strips from the guinea pig lower esophageal sphincter caused by DNP, ANP, brain natriuretic peptide (BNP), C-type natriuretic peptide (CNP), and a natriuretic peptide receptor-C agonist des[Gln^1^8, Ser^1^9, Gly^2^0, Leu^2^1, Gly^2^2]ANP(4-23) amide (cANF(4-23)) in vitro. In resting strips of the guinea pig lower esophageal sphincter DNP and BNP caused marked relaxations. Furthermore, in both sarafotoxin S6c and carbachol-contracted lower esophageal sphincter strips, DNP caused marked and BNP caused moderate, concentration-de...

UK PubMed Central (United Kingdom)

BackgroundMammalian antimicrobial peptides (AMPs) are effectors of the innate immune response. A multitude of signals coming from pathways of mammalian pathogen/pattern recognition...Full Text Available

UK PubMed Central (United Kingdom)

Background: The causes of elevated B-Type natriuretic peptide (BNP) levels are multifactorial. Renal dysfunction has been shown to affect BNP levels in some studies and...Full Text Available

UK PubMed Central (United Kingdom)

In the cellular immune response, recognition by CTL-TCRs of viral antigens presented as peptides by HLA class I molecules, triggers destruction of the virally infected cell (Townsend, A.R.M., J. Rothbard,...Full Text Available

International Nuclear Information System (INIS)

IRAN.LIB is a Coupled Neutron-Gamma library which was developed to be used by the ANISN/PC (ccc-0514/02). The library was developed mainly for shielding calculations and it contains 33 elements mostly used as shielding materials, such as concrete. IRAN.LIB is a set of six libraries, each having the same elements but different number of energy groups. In order to use IRAN.LIB by ANISN/PC, this code must be modified. (author).

KoreaScience (Korea)

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UK PubMed Central (United Kingdom)

A new proprietary de novo peptide design technique generated ten 15-residue peptides targeting and containing the leading nontransmembrane hydrophobic autocorrelation wavelengths, “modes”,...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Alzheimer's disease (AD) is a neurodegenerative disorder for which the research of new treatments is highly challenging. Since the fibrillogenesis of amyloid-b peptide 1-42 (Ab1-42) peptide is considered as a major cause of neuronal degeneration, specific interest has been focused on aromatic molecules for targeting this peptide. In this paper, the synthesis of selegiline-functionalized and fluorescent poly(alkyl cyanoacrylate) nanoparticles (NPs) and their evaluation for the targeting of the Ab1-42 peptide are reported. The synthetic strategy relied on the design of amphiphilic copolymers by tandem Knoevenagel-Michael addition of cyanoacetate derivatives, followed by their self-assembly in aqueous solutions to give the corresponding NPs. Different cyanoacetates were used: (i) hexadecyl cy...

International Nuclear Information System (INIS)

Cholera toxin catalyzes transfer of radiolabel from ["3"2P]NAD"+ to several peptides in particulate preparations of human foreskin fibroblasts. Resolution of these peptides by two-dimensional gel electrophoresis allowed identification of two peptides of M/sub r/ = 42,000 and 52,000 as peptide subunits of a regulatory component of adenylate cyclase. The radiolabeling of another group of peptides (M/sub r/ = 50,000 to 65,000) suggested that cholera toxin could catalyze ADP-ribosylation of cytoskeletal proteins. This suggestion was confirmed by showing that incubation with cholera toxin and ["3"2P]NAD"+ caused radiolabeling of purified microtubule and intermediate filament proteins.

Science.gov (United States)

Synthetic atrial natriuretic peptide (carperitide) and B-type natriuretic peptide (BNP; nesiritide) are used to treat congestive heart failure. However, despite beneficial cardiac unloading properties, reductions in renal perfusion pressures limit their clinical effectiveness. Recently, CD-NP, a chimeric peptide composed of C-type natriuretic peptide (CNP) fused to the C-terminal tail of Dendroaspis natriuretic peptide (DNP), was shown to be more glomerular filtration rate-enhancing than BNP in dogs. However, the molecular basis for the increased responsiveness was not determined. Here, we show that the DNP tail has a striking effect on CNP, converting it from a non-agonist to a partial agonist of natriuretic peptide receptor (NPR)-A while maintaining the ability to activate NPR-B. This effect is specific for human receptors because CD-NP was only a slightly ...

UK PubMed Central (United Kingdom)

As the Medical Library Association (MLA) enters its second century, its role in providing leadership and focus for the education of health information professionals in a changing environment will be...Full Text Available

UK PubMed Central (United Kingdom)

A brief profile of Janet Doe is given. Twenty-five years of library automation are reviewed from the author's point of view. Major projects such as the SUNY Biomedical Communication Network and the...Full Text Available

UK PubMed Central (United Kingdom)

Objective: The paper describes the information needs of a state public health agency, compares needs to its library's collection, and evaluates collection development policy accordingly.Methods:...Full Text Available

Science.gov (United States)

sharing data and computational resources, and accessing information in digital libraries." Bill Wulf (1989) In a Collaboratory, the presentation/meeting concept embodied...

Science.gov (United States)

... 15, 14, Robert Constable, Cornell University, NY, Building Interactive Digital Libraries of Formal Algorithmic Knowledge, Navy. ...

Science.gov (United States)

Featured Collections Featured collections are information sources that use federated search or are known for presenting exceptional, authoritative information for...

Energy Technology Data Exchange (ETDEWEB)

Sandia National Laboratories maintains several libraries of equation of state tables, in a modified Sesame format, for use in hydrocode calculations and other applications. This report discusses one of those libraries, the seslan file, which contains 78 tables from the Los Alamos equation of state library. Minor changes have been made to these tables, making them more convenient for code users and reducing numerical difficulties that occasionally arise in hydrocode calculations.

Energy Technology Data Exchange (ETDEWEB)

The term "homology" or "homologous" means an amino acid similarity measured by the program, BLAST (Altschul et al (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:33 89 3402), and expressed as --(% identity n/n). In measuring homology between a peptide and a protein of greater size, homology is measured only in the corresponding region; that is, the protein is regarded as only having the same general length as the peptide, allowing for gaps and insertions.

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to show that the need to communicate the value of libraries is growing, and especially now during the global financial crisis. As a response library valuation research is expanding and there is now a need for a status report. Design/methodology/approach - The library valuation field is developing towards generating a critical mass of empirical studies. The focus of the meta-analytical review is on the subgroup that reports a return on investment (ROI) or a cost-benefit ratio. Meta-analysis is a quantitative analysis of findings of previous studies, conducted to infer general findings and lessons from prior empirical research. The dataset is 38 library valuation studies reporting a return on investment figure or cost-benefit ratio. Findings - Of the 38...

Science.gov (United States)

This manual is intended to provide a free resource on essential network security concepts for non-technical managers of small libraries. Managers of other small nonprofit or community organizations will also benefit from it. An introduction defines network security; outlines three goals of network security; discusses why a library should be concerned with network security; and describes limits of this work. The manual is divided into three main parts. Part One features the management issues related to network security: analyzing risk, developing a security plan and policy, the funding requirements libraries can expect in operating their networks, and implementing adequate security. Part Two describes the areas of computer networks that need to be secured, and provides a description of many of the security measures necessary for adequate security. Part Three presents sample documents that may be help in the ...

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to encourage other libraries to provide access to their own original content via the web. By way of case study, the process of identifying materials, scanning them and creating web access to original content will be explored. Design/methodology/approach - To implement a book digitization project, three major things need to be present. The library must own material that was published prior to 1923, making them safely out of copyright in the USA; the library needs access to server space on the web and the library needs to own scanning equipment. Findings - This project demonstrated that a library does not necessarily need to obtain grant funding to begin an effective and successful digitization program. It also revealed a few things that would improve t...

UK PubMed Central (United Kingdom)

In an effort to develop octahedral metal complexes as chemotherapeutic and diagnostic agents targeted to DNA, it is critical to optimize the properties of their cellular uptake. Appending d-octaarginine...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

Science.gov (United States)

Digital libraries play a crucial role in distance learning. Nowadays, they are one of the fundamental information sources for the students enrolled in this learning system. These libraries contain huge amount of instructional data (text, audio and video) offered by the distance learning program. Organization of the digital libraries is therefore very important for easy and fast access to the desired information. Improper categorization of data may mislead the students searching the library. Since manual categorization of huge amount of data might be challenging, an automatic and reliable method is needed. In this sense, this paper proposes an automated categorization scheme for digital libraries in distance learning. The categorization scheme is designed and developed by a pattern recognition approach. Effectiveness of the proposed scheme is evaluated on widely used Reuters ...

International Nuclear Information System (INIS)

The effects of D-amino acids at Asp"2"3 and Ser"2"6 residues on the conformational preference of #beta#-amyloid (A#beta#) peptide fragment (A#beta#_2_0_-_2_9) have been studied using different spectroscopic techniques, namely vibrational circular dichroism (VCD), vibrational absorption, and electronic circular dichroism. To study the structure of the A#beta#_2_0_-_2_9, [D-Asp"2"3]A#beta#_2_0_-_2_9, and [D-Ser"2"6]A#beta#_2_0_-_2_9 peptides under different conditions, the spectra were measured in 10 mM acetate buffer (pH 3) and in 2,2,2-trifluoroethanol (TFE). The spectroscopic results indicated that at pH 3, A#beta#_2_0_-_2_9 peptide takes random coil with #beta#-turn structure, while [D-Ser"2"6]A#beta#_2_0_-_2_9 peptide adopts significant amount of polyproline II (PPII) type structure along with #beta#-turn contribution and D-Asp-substituted peptide ([D-Asp"2"3]A#beta#_2_0_-_2_9) ...

DEFF Research Database (Denmark)

Staphylococcus aureus survives inside eukaryotic cells. Our objective was to assess the activity of NZ2114, a novel peptidic antibiotic, against intracellular S. aureus in comparison with established antistaphylococcal agents acting on the bacterial envelope with a distinct mechanism.

Science.gov (United States)

CD-NP is a novel chimeric natriuretic peptide (NP) consisting of the 22-amino-acid (AA) human C-type natriuretic peptide (CNP), a venodilating peptide with limited renal actions and minimal effects on blood pressure, and the 15-AA C-terminus of Dendroaspis NP (DNP). The rationale for the design of CD-NP was to enhance the renal actions of CNP, the ligand for natriuretic peptide receptor-B, but without inducing excessive hypotension. Here we report the first-in-human studies for CD-NP, which represent the first successful clinical testing of a chimeric NP demonstrating in normal human volunteers that CD-NP possesses cyclic guanosine monophosphate-activating, natriuretic, and aldosterone-suppressing properties without inducing excessive hypotension, laying the foundation for additional studies on this first-in-class new cardiovascular therapeutic in human heart failure, which are now underway worldwide. ...

Science.gov (United States)

Directed evolution of proteins depends on the production of molecular diversity by random mutagenesis. While a number of methods have been developed for introducing this diversity, the best ways to sample it are not always clear. Here we used simple statistics to analyse completeness and diversity in randomized libraries generated by oligonucleotide-directed mutagenesis, error-prone polymerase chain reaction (epPCR) and in vitro recombination of highly homologous sequences. For oligonucleotide-directed mutagenesis, we derive equations to estimate how complete a given library is expected to be and also to predict the size of library required to give a fixed probability of being 100% complete. We describe the statistical bases for computer programs which estimate the number of distinct variants represented in epPCR and shuffled libraries, dubbed PEDEL and DRIVeR, respectively. These programs allow the ...

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to describe the processes and procedures utilized by Jacksonville State University's Houston Cole Library to digitize an oral history collection and provide access to it through the Library's catalog, web presence, and the state-wide digital repository initiative, Alabama Mosaic (see www.alabamamosaic.org). Design/methodology/approach - The paper is based on the Library's experiences in acquiring, digitizing, creating metadata for and making accessible a collection of oral histories. Findings - The paper describes the obstacles overcome, issues encountered, procedures established, and ramifications of the project. Practical implications - Many institutions are currently digitizing unique collections as part of repository initiatives, both institutiona...

British Library Electronic Table of Contents (United Kingdom)

Abstract An important aspect of Chinese academic health science libraries is their involvement in teaching medical information retrieval courses as part of the medical curriculum. Health science librarians in China have a more formal teaching role than is generally found in Western countries, including many full time teaching positions. This article provides a case study of Kunming Medical University Library, where courses are provided as credit units at both undergraduate and postgraduate levels. The teaching practices of Chinese health science libraries are compared with teaching experiences reported in Western countries. It is noted that Chinese government s educational policy is similar to that of the United States in promoting the role of the library in teaching subjects as part of th...

Science.gov (United States)

Inventory Project home page Reports, Posters, Presentations Internet Tools - Digital Libraries, IMS's Maps, Aerial Photographs Meetings & Conferences Photo Tours - all...

Science.gov (United States)

Market based pricing only applies to the extent it is consistent with these other requirements. For example: .... work, market based pricing does not apply. ...

Science.gov (United States)

... 6(2): 36-45. Hedstrom, M. (1998). Digital preservation: a time bomb for digital libraries. [Online]. Available: http://www.uky ...

UK PubMed Central (United Kingdom)

Objective:This paper describes the Lamar Soutter Library's process and costs associated with digitizing 300 doctoral dissertations for a newly implemented institutional repository...Full Text Available

Science.gov (United States)

... Topics includes: Formal methods in information engineering, Electronic documents and digital libraries Autonomous robots in embeded systems ...

Science.gov (United States)

Home A service of the U.S. National Library of Medicine® Home Conditions Genes Chromosomes Handbook Glossary Resources Genetic Conditions > Browse Conditions 1-9 | A | B | C...

Science.gov (United States)

site for reference information. Other recipients include Google's Translator, WikiLeaks, The ICUN Red List of Threatened Species, and the Public Library of Science (PLOS)....

International Nuclear Information System (INIS)

The interpretation of data from the nuclear forensic analysis of illicit nuclear material of unknown origin requires comparative data from samples of known origin. One way to provide such comparative data is to create a system of national nuclear forensics libraries, in which each participating country stores information about nuclear or other radioactive material that either resides in or was manufactured by that country. Such national libraries could provide an authoritative record of the material located in or produced by a particular country, and thus forms an essential prerequisite for a government to investigate illicit uses of nuclear or other radioactive material within its borders. We describe the concept of the national nuclear forensic library, recommendations for content and structure, and suggested querying methods for utilizing the information for addressing nuclear smuggling.

Science.gov (United States)

In APCs, presentation by MHC II molecules of the chemically dominant peptide from the protein hen egg white lysozyme (HEL) generates different conformational isomers of the peptide-MHC II complexes (pMHC). Type B pMHCs are formed in early endosomes from exogenous peptides in the absence of H2-DM, whereas in contrast, type A pMHC complexes are formed from HEL protein in late vesicles after editing by H2-DM. Thus, H2-DM edits off the more unstable pMHC complexes, which are not presented from HEL. In this study, we show that type B pMHC complexes were presented from HEL protein only after stimulation of dendritic cells (DC) with TLR ligands or type I IFN. Type I IFN contributed to most TLR ligand-induced type B pMHC generation, as presentation decreased in DC lacking the receptor for type I IFNs (IFNAR1(-/-)). In contrast, presentation of type A pMHC from HEL and from peptide was minimally affected by TLR ...

Energy Technology Data Exchange (ETDEWEB)

In a given species, injected opioid peptides usually cause changes in temperature similar to those caused by nonpeptide opioids. The main effect in those species most studied, the cat, rat, and mouse, is an increase in the level about which body temperature is regulated; there is a coordinated change in the activity of thermoregulatory effectors such that hyperthermia is produced in both hot and cold environments. Larger doses may depress thermoregulation, thereby causing body temperature to decrease in the cold. Elicitation of different patterns of response over a range of environmental temperatures and studies with naloxone and naltrexone indicate that stimulation of a number of different receptors by both peptide and nonpeptide opioids can evoke thermoregulatory responses. ..beta..-Endorphin is readily antagonized by naloxone whereas methionine-enkephalin can act on naloxone-insensitive receptors. Moreover, synthetic ...

International Nuclear Information System (INIS)

Antibodies to hemoglobin have been studied with a radioimmunoassay which employs ["1"4C]carbamylated (= carbamoylated) hemoglobin S. An antiserum raised against hemoglobin S, which initially discriminated poorly between hemoglobins S and A, was fractionated by absorption to a column of Sepharose to which a synthetic peptide corresponding to the first 13 amino-acid residues of the #beta# chain of sickle hemoglobin had been covalently bound. A subpopulation of the antiserum was eluted from this column with 4 M guanidine . HCl. These antibodies showed binding to hemoglobin S but not to hemoglobin A and this interaction could be inhibited by the synthetic peptide. These antibodies, of demonstrated fine structural specificity, may be useful in the detection of sickle hemoglobin and in the study of its structure in solution.

British Library Electronic Table of Contents (United Kingdom)

The atmospheric processing by ozone of peptide-containing mixed particles was investigated as proxies for biogenic and sea spray primary organic aerosol. Reactions were performed in a flow reactor and particle composition was monitored by photoelectron resonance capture ionization aerosol mass spectrometry. Mixed particles containing dipeptides in a saturated organic matrix of stearic and palmitic acids showed no reaction under ozonolysis at exposure levels of 2.5???10?4?atm s O3. However reactions of mixed particles of a dipeptide (Leu-Leu) in an unsaturated matrix (oleic acid) under the same conditions resulted in a rapid loss of the peptide ion signal, as well as the carrier matrix, and appearance of a number of ion signals corresponding to secondary products. High molecular weight imid...

Energy Technology Data Exchange (ETDEWEB)

This document contains papers which were presented at the First ACM International Conference on Digital Libraries. Topics processed for this document included information retrieval and index structures for structured documents. Individual papers have been processed separately for the United States Department of Energy databases.

Science.gov (United States)

There are many methods for introducing random mutations into nucleic acid sequences. Previously, we described a suite of programmes for estimating the completeness and diversity of randomized DNA libraries generated by a number of these protocols. Our programmes suggested some empirical guidelines for library design; however, no information was provided regarding library diversity at the protein (rather than DNA) level. We have now updated our web server, enabling analysis of translated libraries constructed by site-saturation mutagenesis and error-prone PCR (epPCR). We introduce GLUE-Including Translation (GLUE-IT), which finds the expected amino acid completeness of libraries in which up to six codons have been independently varied (according to any user-specified randomization scheme). We provide two tools for assisting with experimental design: CodonCalculator, for assessing ...

CERN Document Server

The field of digital libraries (DLs) coalesced in 1994: the first digital library conferences were held that year, awareness of the World Wide Web was accelerating, and the National Science Foundation awarded $24 Million (U.S.) for the Digital Library Initiative (DLI). In this paper we examine the state of the DL domain after a decade of activity by applying social network analysis to the co-authorship network of the past ACM, IEEE, and joint ACM/IEEE digital library conferences. We base our analysis on a common binary undirectional network model to represent the co-authorship network, and from it we extract several established network measures. We also introduce a weighted directional network model to represent the co-authorship network, for which we define $AuthorRank$ as an indicator of the impact of an individual author in the network. The results are validated against conference program committee ...

UK PubMed Central (United Kingdom)

The acyl coenzyme A (CoA) binding protein AcbA is secreted unconventionally and processed into spore differentiation factor 2 (SDF-2), a peptide that coordinates sporulation in Dictyostelium...Full Text Available

UK PubMed Central (United Kingdom)

1. Seven unique carboxymethylcysteine-containing peptides have been isolated from tryptic digests of rabbit muscle aldolase carboxymethylated with iodo[2-¹⁴C]acetic acid in 8m-urea....Full Text Available

CERN Document Server

We have performed multicanonical Monte Carlo simulations of C-peptide of ribonuclease A. It is known by CD and NMR experiments that this peptide has high alpha-helix content in aqueous solution and that the side-chain charges of residues Glu-2$^-$ and His-12$^+$ play an important role in the stability of the alpha-helix. In order to confirm these experimental implications, we have used two analogues of the peptide with charged and neutral side chains of Glu-2 and His-12. Two dielectric functions, distance-dependent and constant, are considered to study the effects of solvent contributions. All the simulations were started from random initial conformations. Various thermodynamic quantities such as average helicity as a function of residue number and average distance between two side chains as a function of temperature are calculated. The results are found to be in accord with the implications of CD and NMR experiments. The ...

UK PubMed Central (United Kingdom)

Peptide toxins with high affinity, divergent pharmacological functions, and isoform-specific selectivity are powerful tools for investigating the structure-function relationships of voltage-gated...Full Text Available

UK PubMed Central (United Kingdom)

In this work, we explore the potential of the metalloendopeptidase Lys-N for MALDI-MS/MS proteomics applications. Initially we digested a HEK293 cellular lysate with Lys-N and, for comparison, in parallel...Full Text Available

UK PubMed Central (United Kingdom)

The neonatal Fc receptor FcRn provides IgG molecules with their characteristically long half-lives in vivo by protecting them from intracellular catabolism and then returning them to...Full Text Available

UK PubMed Central (United Kingdom)

The parathyroid hormone receptor (PTHR) is a class B G protein-coupled receptor that plays critical roles in bone and mineral ion metabolism. Ligand binding to the PTHR involves interactions to both...Full Text Available

UK PubMed Central (United Kingdom)

Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus that cost nearly 800 lives. While there have been no recent outbreaks of the disease, the threat...Full Text Available

UK PubMed Central (United Kingdom)

We have developed a method for dissecting single neurons from the nematode Ascaris suum, in order to determine their peptide content by mass spectrometry (MS). In this paper,...Full Text Available

UK PubMed Central (United Kingdom)

Type 2 diabetes mellitus (T2DM) results from insulin resistance and β-cell dysfunction, in the setting of hyperglucagonemia. Glucagon is a 29 amino acid peptide hormone, which is secreted from...Full Text Available

International Nuclear Information System (INIS)

In the synthesis of the peptidyl-polymer corresponding to the sequence (57-100) of the #beta# chain of human hemoglobin on Teflon with radiationally grafted polystyrene a comparative study was made of different methods of fragment condensation. It was shown that the most effective methods are azide condensation and condensation with the use of complex F.

UK PubMed Central (United Kingdom)

The carboxyl-terminal region of tubulin alpha and beta subunits plays a major role in regulating its assembly into microtubules and constitutes an essential domain for the selective interaction of microtubule-associated...Full Text Available

UK PubMed Central (United Kingdom)

OBJECTIVEPreimplantation factor (PIF) is a novel, 15 amino acid peptide, secreted by viable embryos. This study aims to elucidate PIF’s effects in human endometrial...Full Text Available

UK PubMed Central (United Kingdom)

During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes...Full Text Available

Science.gov (United States)

Nine secondary metaboites of cyclic peptide were isolated from three mangrove endophytic fungi Paecilomyces sp. (treel-7), 4557,ZZF65. They were viscumamide(1),cyclo(Pro-Iso)(2),cyclo(Phe-Gly)(3),cyclo(Phe-Ana)(4),cyclo(Gly-Pro) (5),cyclo(Gly-Leu)(6), cyclo(Trp-Ana)(7),neoechinulin A(8),cyclo(Pro-Thr)(9). The compounds 1,7,8,9 were firstly isolated from marine fungus. PMID:18422185

Science.gov (United States)

BackgroundDespite an ever-improving understanding of the molecular biology of cancer, the treatment of most cancers has not changed dramatically in the past three decades and drugs that do not discriminate between tumor cells and normal tissues remain the mainstays of anticancer therapy. Since Hsp90 is typically involved in cell proliferation and survival, this is thought to play a key role in cancer, and Hsp90 has attracted considerable interest in recent years as a potential therapeutic target.MethodsWe focused on the interaction of Hsp90 with its cofactor protein p60/Hop, and engineered a cell-permeable peptidomimetic, termed "hybrid Antp-TPR peptide", modeled on the binding interface between the molecular chaperone Hsp90 and the TPR2A domain of Hop.ResultsIt was demonstrated that this designed hybrid Antp-TPR peptide inhibited the interaction of Hsp90 with the TPR2A domain, inducing cell death of breast, pancreatic, renal, lung, prostate, ...

British Library Electronic Table of Contents (United Kingdom)

Purpose - Customer relationship management (CRM) indicates a comprehensive strategy and an interactive process intended to achieve an optimum balance between corporate investment and the satisfaction of customer needs to generate the maximum profit. E-CRM refers to CRM using internet technology plus a database, OLAP, data warehouse, data mining, etc. In order to gain an understanding of the efficiency of implementing an e-CRM system within the library context, to develop theoretically and empirically an evaluation process for the e-CRM system and survey its impact on service quality, a pilot scheme was initiated in 2004. The pilot scheme was to design and implement an e-CRM prototype system for a particular academic library in Taiwan and to survey the system's performances. This paper aims...

Science.gov (United States)

Explicitly defining virtual collections in a digital library can benefit both users and contributors. This paper outlines the costs and benefits for defining virtual collections and describes an implementation of collection-level metadata in two digital libraries. Our work suggests that component repositories of NSDL would profit by describing their collection, and sub-collections, using a schema such as the one we implemented. This would enable NSDL to provide a rich, cross-linked hierarchy of virtual collections. By sharing collection-level, rather than item-level, metadata, collections would provide detailed resource information, presumably keeping most of their viewership, while NSDL would provide a broad description of resources.

International Nuclear Information System (INIS)

As a contribution to the US/Japan cooperative program in fusion neutronics, we have prepared a library of multigroup neutron cross sections, scattering matrices, and covariances (uncertainties and their correlations). This 74-group library, called COVFILS-2, is being used at Los Alamos and at the University of California at Los Angeles in the sensitivity and uncertainty analysis of the Li_2O integral experiment recently performed at the Fast Neutron Source (FNS) in Japan. Another intended use of this library is in the estimation of the uncertainty in key performance parameters (such as breeding ratio) of conceptual fusion reactors. The 14 materials included in the first version of COVFILS-2 are H, "6Li, "7Li, Be, C, N, O, Na, Al, Si, Cr, Fe, Ni, and Pb.

UK PubMed Central (United Kingdom)

The viroid and viroid-like RNA database is a compilation of all natural sequences published in journals or available from the GenBank and EMBL nucleotide sequence libraries. Several information regarding...Full Text Available

UK PubMed Central (United Kingdom)

A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundPhysicians have difficulty keeping up with new evidence from medical research.MethodsWe developed the McMaster Premium...Full Text Available

UK PubMed Central (United Kingdom)

The University of Cincinnati (UC) has been active in the National Library of Medicine's Integrated Advanced Information Management Systems (IAIMS) program since IAIMS' inception in 1984. UC received...Full Text Available

UK PubMed Central (United Kingdom)

The concept of "one-stop information shopping" is becoming a reality at Columbia-Presbyterian Medical Center. Our goal is to provide access from a single workstation to clinical, research, and library...Full Text Available

UK PubMed Central (United Kingdom)

Increasing numbers of human cowpox virus infections that are being observed and that particularly affect young non-vaccinated persons have renewed interest in this zoonotic disease. Usually causing...Full Text Available

Science.gov (United States)

Install. Inspect. Protect. Smoke Alarm Campaign Video 10/28/2009 | 04:09 Cooking_4 Cooking Fire Safety: Watch What You Heat 10/26/2007 | 01:40 Cooking_3 Cooking Fire...

Science.gov (United States)

Ever-changing audio technology requires that National Library Service NLS always be aware of developments and prepare carefully for any systematic changes that may be desired or required. Usefulness, cost effectiveness, thoughtful stewardship, and educate...

Energy Technology Data Exchange (ETDEWEB)

The authors describe a cloning strategy for the construction of a human genomic library in yeast artificial chromosomes (YACs) based on complete digestion of high-molecular-weight DNA with the infrequently cutting restriction enzyme MluI. Cloning of MluI fragments in the vector pYAC-RC and one subsequent size fractionation by preparative pulsed-field gel electrophoresis yielded a library with average insert sizes of 600 kb. Ninety-seven percent of the colonies were recombinant. An additional size fractionation of MluI fragments prior to ligation had no significant influence on the size of the YACs. The library currently consists of 5000 clones, which is the equivalent of one human genome. Nineteen percent of the YACs were larger than 1.2 Mb. Since smaller MluI fragments are lost during sizing, they also performed cloning without size fractionation. Only 20% of the colonies were recombinant, probably due to unligated vector ...

UK PubMed Central (United Kingdom)

The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase...Full Text Available

Wastenet

Heidelberg: Springer-Verlag. ABRAHART, R.J., SEE, L.& DAWSON, C. ...Water Science and Technology Library: Springer. AL-AHMADI, K., HEPPENSTALL, A.J., ...Springer. BIRKIN, M.H., TURNER, A., WU, B.

UK PubMed Central (United Kingdom)

A cDNA encoding a cytochrome P450 enzyme was isolated from a cDNA library of the corpora allata (CA) from reproductively active Diploptera punctata cockroaches. This P450 from the endocrine...Full Text Available

International Nuclear Information System (INIS)

An improved labeling procedure for peptides attached to organometallic 99mTc(III) '4+1' mixed-ligand complexes in which the radiometal is coordinated by a tripodal tetradentate chelator 2,2',2''-nitrilotriethanethiol (NS3) and a monodentate isocyanide ligand is presented. The labeling procedure was evaluated by the synthesis of [99mTc(NS3)(L2-RGD)]. The containing radiopharmaceutically interesting RGD-peptide cyclo[Arg-Gly-Asp-D-Tyr-Lys] was modified with 4-isocyanobutanoic acid (L2) as linker conjugated to N6-Lys to get the monodentate ligand L2-RGD. The structural identity of the 99mTc-conjugate was confirmed by comparison to a Re reference compound. The Tc- and Re-conjugates had matching retention times under identical HPLC conditions. The 99mTc-labeling was performed in a novel one-step procedure using the eluate of a 99Mo/99mTc generator, NS3, the isocyanide modified peptide, SnCl2, Na2EDTA, mannitol and ascorbic acid ...

Wastenet

... John Cannaday and Ken Weddle present the framework for a Product Design Engineering project (http://engineering.dartmouth.edu/~teps/images/ProductDesign.pdf) for high school students which may have relevance at a more advanced level. David Price has created a Mechanical Engineering Design (http://www.soton.ac.uk/~cds/) site containing engineering design notes. The Virtual Reality Engineering Library Home Page at the University of Bristol (http://www.dig.bris.ac.uk/virtual_lib/) is a library ...

Energy Technology Data Exchange (ETDEWEB)

Information glut is one of the primary characteristics of the electronic age. Managing such large volumes of information (e.g., keeping track of the types, where they are, their relationships, who controls them, etc.) can be done efficiently with an intelligent, user-oriented information management system. The purpose of this paper is to describe a concept for managing information resources based on an intelligent information technology system developed by the Argonne National Laboratory for managing digital libraries. The Argonne system, Intelligent Query (IQ), enables users to query digital libraries and view the holdings that match the query from different perspectives.

CERN Document Server

Developing in Ext GWT is a fast--paced, practical guide to quickly learning the tasks necessary in building enterprise--class Rich Internet Applications. Based around the exciting new UI library from Ajax leaders Ext JS and the latest GWT release, this book takes the reader through setup, the available widgets, and advanced custom widgets and templates, and concludes with a functional sample client--server application in less than 150 pages. Not your typical beginner's guide to programming, this book provides a rapid approach to becoming effective with leading commercial RIA tools and librarie

CERN Document Server

Zinc-fingers, which widely exist in eukaryotic cell and play crucial roles in life processes, depend on the binding of zinc ion for their proper folding. To computationally study the zinc coupled folding of the zinc-fingers, charge transfer and metal induced protonation/deprotonation effects have to be considered. Here, by attempting to implicitly account for such effects in classical molecular dynamics and performing intensive simulations with explicit solvent for the peptides with and without zinc binding, we investigate the folding of the Cys2His2 type zinc-finger motif and the coupling between the peptide folding and zinc binding. We find that zinc ion not only stabilizes the native structure, but also participates in the whole folding process. It binds to the peptide at early stage of folding, and directs or modulates the folding and stabilizations of the component beta-hairpin and alpha-helix. Such a crucial role of ...

British Library Electronic Table of Contents (United Kingdom)

The photoelectron resonance capture ionization (PERCI) of cysteine (Cys) and small gas-phase neutral peptides that contain the Cys residue (Cys-Gly, g-Glu-Cys, and glutathione (g-Glu-Cys-Gly)) is reported. At an ionization energy less than 1eV two types of dissociative electron attachment ionization were observed for Cys: hydrogen atom loss, resulting in formation of the ion [Cys-H]-, and dissociation of the CH2-SH bond, resulting in formation of the ion [SH]-. The presence of these ions suggests that both the p*(-CO2H) and *(C-S) orbitals can act as low energy electrophores on Cys. This ionization trend was observed for the dipeptides Cys-Gly and g-Glu-Cys as well as glutathione, with evidence that dissociation of the CH2-SH bond in these peptides can also result in ions of the form [M-SH...

International Nuclear Information System (INIS)

Two peptide ligands conjugated adenine, [9-N-(tritylmercapto acetyl diglycyl aminoethyl) adenine, Tr-MAG_2-Ade] and [9-N-(tritylmercapto acetyl triglycyl aminoethyl) adenine, Tr-MAG3-Ade], are synthesized and labeled with "9"9"mTc by directly labeling method. The stability of "9"9"mTc-MAG_2- adenine and "9"9"mTc-MAG_3-adenine in vitro is measured. The uptake radios of tumor to muscle at 3h post-injection are 5.70 and 4.92, respectively. The biodistribution and scintigraphic imaging studies show that the two complexes have high localization in tumor and high contrasted tumor images can be obtained, which suggest their potential utility as tumor imaging agents. But the high radioactivity of abdomen could prevent the tumor imaging in this area. (author)

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

British Library Electronic Table of Contents (United Kingdom)

A series of [60]fullerene-substituted phenylalanine (Baa) and lysine derivatives have been prepared by the condensation of 1,2-(4prime-oxocyclohexano)fullerene with the appropriately protected (4-amino)phenylalanine and lysine, respectively. Conversion of the imine to the corresponding amine is achieved by di-acid catalyzed hydroboration. The reduction of the imine is not accompanied by hydroboration of the fullerene cage. The [70]fullerene phenylalanine derivative has also been prepared as have the di-amino acid derivatives. The compounds were characterized by MALDI-TOF mass spectrometry, UV/Vis spectroscopy, and cyclic voltammetry. 1H and 13C NMR spectroscopy allowed the observation of diastereomers. Fullerene-substituted peptides may be synthesized on relatively large scale by solid-pha...

British Library Electronic Table of Contents (United Kingdom)

Apelin is a peptide known as the ligand of the G-protein-coupled receptor APJ. Several active apelin forms exist such as apelin-36, apelin-17, apelin-13, and the pyroglutamated form of apelin-13. Apelin and APJ are expressed in the central nervous system, particularly in the hypothalamus and in many peripheral tissues. Apelin has been shown to be involved in the regulation of cardiovascular and fluid homeostasis, food intake, cell proliferation, and angiogenesis. In addition to be an ubiquitous peptide, apelin is also produced and secreted by adipocytes and thus considered as an adipokine. This has opened a new field of investigation establishing a link between apelin and metabolic disorders (obesity, type 2 diabetes, etc.) which is the focus of the present review. Several studies, but not...

Science.gov (United States)

The complexity and diversity of the microbial communities in biogranules from an upflow anaerobic sludge blanket (UASB) bioreactor were determined in response to short-term changes in substrate feeds. The reactor was fed simulated brewery wastewater (SBWW) (70% ethanol, 15% acetate, 15% propionate) for 1.5 months (phase 1), acetate / sulfate for 2 months (phase 2), acetate-alone for 3 months (phase 3), and then a return to SBWW for 2 months (phase 4). Performance of the reactor remained relatively stable throughout the experiment as shown by COD removal and gas production. 16S rDNA, methanogen-associated mcrA and sulfate reducer-associated dsrAB genes were PCR amplified, then cloned and sequenced. Sequence analysis of 16S clone libraries showed a relatively simple community composed mainly of the methanogenic Archaea (Methanobacterium and Methanosaeta), members of the Green Non-Sulfur (Chloroflexi) group of Bacteria, followed by fewer numbers of Syntrophobacter, ...

Energy Technology Data Exchange (ETDEWEB)

In the present study, the immunomodulatory effect of {beta}-endorphin ({beta}-E) and shorter pro-opiomelancortin (POMC) fragments was evaluated by assessing their influence on respiratory burst in human polymorphonuclear leukocytes (PMN). The effect of the peptides on phorbol myristate acetate (PMA)-stimulated production of reactive oxygen metabolites was measured in a lucigenin-enhanced chemiluminescence (CL) assay. Both POMC peptides with opiate-like activity and their non-opioid derivatives were tested. With the exception of {alpha}-E, PMA-stimulated respiratory burst was suppressed by all POMC fragments tested. A U-shaped dose-response relation was observed. Doses lower than 10{sup {minus}17}M and higher than 10{sup {minus}8}M were without effect. {beta}-E and dT{beta}E both suppressed PMA-induced oxidative burst in human PMN at physiological concentrations. {gamma}-E and dT{gamma}E proved to be less potent inhibitors, reaching maximal ...

UK PubMed Central (United Kingdom)

BackgroundOur previous studies indicate that either PEP-1-superoxide dismutase 1 (SOD1) or PEP-1-catalase (CAT) fusion proteins protects myocardium from ischemia-reperfusion-induced...Full Text Available

UK PubMed Central (United Kingdom)

The management of CNS tumors is limited by the blood-brain barrier (BBB), a vascular interface that restricts the passage of most molecules from the blood into the brain. Here we show that phage particles...Full Text Available

Science.gov (United States)

There are about fifty SET domain protein methyltransferases (PMTs) in the human genome, that transfer a methyl group from S-adenosyl-L-methionine (SAM) to substrate lysines on histone tails or other peptides. A number of structures in complex with cofactor, substrate, or inhibitors revealed the mechanisms of substrate recognition, methylation state specificity, and chemical inhibition. Based on these structures, we review the structural chemistry of SET domain PMTs, and propose general concepts towards the development of selective inhibitors.

UK PubMed Central (United Kingdom)

CAP18 (an 18-kDa cationic antimicrobial protein) is a granulocyte-derived protein that can bind lipopolysaccharide (LPS) and inhibit various activities of LPS in vitro. The present study examined the...Full Text Available

UK PubMed Central (United Kingdom)

Low-level lead exposure during early childhood has long been associated with altered neurocognitive development and diminished cognitive functions. Over nine thousand U.S. industrial facilities...Full Text Available

Environmental Research Database

DescriptionIron deficiency anaemia is a common problem and may be particularly important to certain groups of vegetarians. This project investigates isolating and identifying iron-binding constituents of meat digests using human Caco-2 cell lines. Stable isotope techniques used to test whether one or more of these compounds enhance iron absorpion in vivo and could therefore be added to vegetarian products to imporve iron nutrition.

UK PubMed Central (United Kingdom)

BackgroundThe human endogenous retrovirus HERV-K(HML-2) family is associated with testicular germ cell tumors (GCT). Various HML-2 proviruses encode viral proteins such as Env and...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

During this report period our research efforts have concentrated on studies of the dissociation reactions of model peptides and other biologically important molecules. In addition, a considerable amount of research effort has been directed toward improving the apparatus used for laser-ion beam photodissociation. The instrumental improvements include some changes on the original apparatus, but most of this effort involved designing a second generation laser-ion beam photodissociation instrument.

International Nuclear Information System (INIS)

Several examples of the 14-membered, para-bridged ring system of the cyclopeptide alkaloids have been synthesized via an active ester cyclization. The yield of monomeric cyclopeptide varied from 1 to 33% and was affected by the amino acid substitution pattern and amide conformation of the linear peptide precursors. Both the synthetic models and a naturally occurring cyclopeptide alkaloid, ceanothine B, bind monovalent (Li"+) and divalent (Ca"2"+, Mg"2"+) cations. 4 figures, 1 table.

UK PubMed Central (United Kingdom)

This study focuses on the effects of long-term renin-angiotensin system suppression and/or incretin mimetic therapies on the regulation and binding affinity of GLP-1 to its receptor in the coronary...Full Text Available

UK PubMed Central (United Kingdom)

We have purified and characterized two peptides, named KAaH1 and KAaH2 (AaH polypeptides 1 and 2 active on K⁺ channels, where AaH stands for Androctonus australis Hector),...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Burnup calculations with SARC system were carried out to analyse the effects of plutonium build-up on criticality of MTR type research reactor PARR-1 using several WIMSD libraries based on evaluated nuclear data files ENDFB-VI.8, JEF-2.2, JEFF-3.1 and JENDL-3.2. For equilibrium core of the reactor, it was found that a net reactivity of more than 3.5 mk is induced due to build-up of plutonium isotopes during depletion. The plutonium credit amounts to 3% of the length of equilibrium cycle. From the analysis of actinide production in the core during burnup, it was observed that in most of the cases, the amounts of actinides obtained using various cross section libraries agree fairly with each other, however, significant differences were observed for {sup 238}Pu, {sup 241}Pu, {sup 242m}Am, {sup 243}Am, {sup 242}Cm and {sup 244}Cm for some libraries. The actinide chain analysis was conducted to investigate the reasons for the ...

Science.gov (United States)

Designed to provide international coverage of current research in library and information science, this quarterly journal indexes investigations, studies, surveys, and evaluated innovations. Doctoral, post-doctoral, and research staff-level work, and in-house or action research from practicing librarians, information workers, archivists, and documentalists are included in the main section, "Current Research, Development, and Investigation." Each standard entry provides information on personnel, expected duration of the work, funding, references, contacts, aims, and a full history of the project. Entries in the main section are arranged according to the Classification Research Group's "A Classification of Library and Information Science" (1971), and each project is assigned a serial number. Cross-references to main section entries are provided in the Alphabetical Subject Index and the Name Index. Sub-doctoral theses and dissertations are listed ...

International Nuclear Information System (INIS)

The author have prepared a new, fusion-oriented library of multigroup neutron cross sections, scattering matrices, and covariances (uncertainties and correlations). The 74-group library, called COVFILS-2, has been used, or will be used, by neutronics groups at Los Alamos National Lab. (LANL) at the University of California at Los Angeles, and at the Swiss Federal Institute for Reactor Research in the sensitivity and uncertainty analysis of fusion-relevant integral experiments such as the Li_2O experiment performed at the Fast Neutron Source Facility in Japan and the Lithium breeding module experiment planned at the LOTUS facility in Lausanne, Switzerland. Another intended use of this library is in the estimation of the uncertainty in key performance parameters (such as the breeding ratio) of conceptual fusion reactors. The 14 materials included in the first version of COVFILS-2 are hydrogen, "6Li, "7Li, beryllium, carbon, ...

Energy Technology Data Exchange (ETDEWEB)

Previous AIX development environment experience with ASC White and Early Delivery systems UV and UM was leveraged to provide a smooth and robust transition to the Purple development environment. Still, there were three major changes that initially caused serious problems for Purple users. The first was making 64-bit builds of executables the default instead of 32-bit. The second was requiring all executables to use large page memory. The third was the phase-out of the popular, but now defunct, third-party C++ compiler KCC, which required the migration of many codes to IBM's xlC C++ compiler. On Purple, the default build environment changed from 32-bit builds to 64-bit builds in order to enable executables to use the 4GB per processor (32GB per node) memory available, and in order for the MPI library to do collective optimizations that required the larger 64-bit address space. The 64-bit build environment was made default by setting the IBM environment ...

British Library Electronic Table of Contents (United Kingdom)

The thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cDNA library from sea bass (Dicentrarchus labrax), one of the most extensively cultured species in South Mediterranean aquaculture. A total of 1632 ESTs from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ESTs were further processed. The total collection of D. labrax thymus ESTs has been deposited in the EBI-GenBank-DBJ database (GenBank accession numbers from FN565576 to FN566839). The functional classification of ESTs was performed by Gene Ontology and KEGG annotation and, successively, the sequences were analysed using the Immuno...

CERN Document Server

Landscapes are both a synthesis and an expression of national, regional and local cultural heritages. It is therefore very important to develop techniques aimed at cataloguing and archiving their forms. This paper discusses the LDL (Landscape Digital Library) project, a Web accessible database that can present the landscapes of a territory with documentary evidence in a new format and from a new perspective. The method was tested in a case study area of the river Po valley (Northern Italy). The LDL is based on a collection of photographs taken following a systematic grid of survey points identified through topographic cartography; the camera level is that of the human eye. This methodology leads to an innovative landscape archive that differs from surveys carried out through aerial photographs or campaigns aimed at selecting "relevant" points of interest. Further developments and possible uses of the LDL are also discussed.

CERN Document Server

Many secure communication libraries used by distributed systems, such as SSL, TLS, and Kerberos, fail to make a clear distinction between the authentication, session, and communication layers. In this paper we introduce CEDAR, the secure communication library used by the Condor High Throughput Computing software, and present the advantages to a distributed computing system resulting from CEDAR's separation of these layers. Regardless of the authentication method used, CEDAR establishes a secure session key, which has the flexibility to be used for multiple capabilities. We demonstrate how a layered approach to security sessions can avoid round-trips and latency inherent in network authentication. The creation of a distinct session management layer allows for optimizations to improve scalability by way of delegating sessions to other components in the system. This session delegation creates a chain of trust that reduces the overhead of ...

International Nuclear Information System (INIS)

Using indigenously developed computer program, coupled neutron gamma multi-group libraries have been generated from MA TXS-NJOY files. The MATXS format contains all the details for each reaction. Temperature and dilution factor dependent cross-sections have been generated and used in discrete ordinates method for benchmark studies of fast critical experiments. We have analyzed criticality benchmarks, which includes CSEWG fast critical benchmarks, "2"3"3U and Pu spheres and enriched uranium spheres using 30-group neutron cross-sections. Neutron and gamma spectra for spheres with point neutron source at the center, are also calculated with 42-group coupled neutron gamma cross-sections. (author)

CERN Document Server

Logic Programming languages and combinational circuit synthesis tools share a common "combinatorial search over logic formulae" background. This paper attempts to reconnect the two fields with a fresh look at Prolog encodings for the combinatorial objects involved in circuit synthesis. While benefiting from Prolog's fast unification algorithm and built-in backtracking mechanism, efficiency of our search algorithm is ensured by using parallel bitstring operations together with logic variable equality propagation, as a mapping mechanism from primary inputs to the leaves of candidate Leaf-DAGs implementing a combinational circuit specification. After an exhaustive expressiveness comparison of various minimal libraries, a surprising first-runner, Strict Boolean Inequality "<" together with constant function "1" also turns out to have small transistor-count implementations, competitive to NAND-only or NOR-only libraries. As a practical outcome, a ...

UK PubMed Central (United Kingdom)

A cosmid library of DNA from colicin Js-sensitive enteroinvasive Escherichia coli (EIEC) strain O164 was made in colicin Js-resistant strain E. coli VCS257, and colicin...Full Text Available

Wastenet

...understanding sweden publication publication market publications publications inco publications library public-private partnerships publishing publishing platform pufafeed puglia pulmonary diseases pulp puma pumped hydro storage pumped storage plants purity purpose purpose-grown energy crops purposes pv pv added value pv cells pv crystalline cells pv organic and polymer cells pv research pv ...

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... News In Practice UK work Key facts & information Mass Balance Projects Resources & Library Technical Area Contacts Site Map Benchmarking solvent waste in the UK furniture manufacturing industry OrganisationBritish Furniture Manufacturers Ltd Project ReportSolvent Waste in Furniture Manufacturing Website: www.bfmenvironment.co.uk Return to list � RSWT | Website Design Quiet Storm Solutions Ltd Please consider upgrading your browser! If you can see this ...

Science.gov (United States)

Evaluation procedures used to produce sets of evaluated data for the 33 heavy isotopes that fall in the range Z = 90 to Z = 98 are described. At the beginning of the discussion for each individual isotope, a computer-generated listing is given which summarizes the main properties of the data sets that are contained in the evaluation. (RWR)

Energy Technology Data Exchange (ETDEWEB)

Various physics applications involve the computation of the standard hypergeometric function {sub 2}F{sub 1} and its derivatives. Because it is not an intrinsic in the common programming languages, automatic differentiation tools will either differentiate through the code that computes {sub 2}F{sub 1} if that code is available or require the user to provide a hand-written derivative code. We present options for the derivative computation in the context of an ionization problem and compare the approach implemented in the Diamant library to standard methods.

UK PubMed Central (United Kingdom)

Nucleotide sequence analysis of the Escherichia coli chromosomal DNA inserted in the plasmid pLC33-5 of the Clarke and Carbon library [Clarke & Carbon (1976) Cell 9, 91-99] revealed the existence...Full Text Available

Science.gov (United States)

Metadata can be used to support accessibility in connection with improved discovery. ...certain metadata elements provide information about what capabilities are needed--either perceptual-motor, cognitive, socio-economic or geographic--to make use of a resource. This cam improve the ability of users who are disadvantaged in these ways to find resources that meet their needs--and avoid ones [resources] that do not.

Science.gov (United States)

Calcitonin gene-related peptide (CGRP) was localized by an immunocytochemical technique in the thyroid-parathyroid complexes of rat, guinea pig, rabbit, and in normal human thyroids and parathyroids. Human medullary carcinomas and parathyroid adenomas were also studied. In man and all animal species examined CGRP was present in the parafollicular cell, however, in guinea pigs only in small amounts. Except in rabbits, presence of CGRP was demonstrated in nerves of the thyroid and parathyroid capsule as well as in the nerve fibers of the capsular blood vessels. In the thyroid of guinea pigs CGRP was also noted in nerve fibers and in blood vessel walls between follicles. CGRP was also present in the parathyroid glands of rat and man, in nerve fibers localized between parathyroid cells. In rabbit the parafollicular cells between parathyroid cells also expressed CGRP immunoreactivity. No CGRP was noted in the parathyroids of the guinea pig. The proximity of parathyroid ...

Science.gov (United States)

A novel single-chain immunotoxin was constructed by combining a CD33-specific single chain Fv (scFv) antibody fragment with an engineered variant of Pseudomonas exotoxin A (ETA). The variant toxin carries the KDEL peptide at its C-terminus, a cellular peptide mediating improved retrograde transport to the endoplasmic reticulum. The purified recombinant fusion protein induced potent apoptosis of the human myeloid cell lines U937, HL-60 and THP-1. Up to 98% of U937 cells were eliminated after treatment for 72 h with a single dose of 500 ng/ml (c. 7 nmol/l). Killing was antigen-specific and occurred by apoptosis. A control protein, consisting of a CD19-specific scFv antibody fragment fused to the ETA-KDEL toxin, failed to induce death of the CD19-negative cell lines U937, HL-60 and THP-1. The CD33-ETA toxin also mediated apoptosis of fresh patient-derived acute myeloid leukaemia cells from bone marrow and peripheral blood. The pronounced ...

International Nuclear Information System (INIS)

Experimental investigation data are systematized of free radical states and processes in irradiated proteins. The investigation is performed by the radiospectral methods. Results are discussed in detail of the study of free radicals electronic structure of amino acids, peptides and proteins formed by the action of ionizing radiation. The specificity is stressed of the study of monocrystalls of these compounds by the method of electronic paramagnetic resonance. The nature is also studied of primary centres formed under the effect of radiation on biologically important compounds and their subsequent reactions in solid and liquid solutions. Ion-radical states of different functional groups of the protein molecule are studied. Prospects of the study and the role of anion-radicals in biological processes are discusses.

Energy Technology Data Exchange (ETDEWEB)

Neurodegeneration observed in Alzheimer disease (AD) is believed to be related to the toxicity from reactive oxygen species (ROS) produced in the brain by the amyloid-{beta} (A{beta}) protein bound primarily to copper ions. The evidence for an oxidative stress role of A{beta}-Cu redox chemistry is still incomplete. Details of the copper binding site in A{beta} may be critical to the etiology of AD. Here we present the structure determined by combining x-ray absorption spectroscopy (XAS) and density functional theory analysis of A{beta} peptides complexed with Cu{sup 2+} in solution under a range of buffer conditions. Phosphate-buffered saline buffer salt (NaCl) concentration does not affect the high-affinity copper binding mode but alters the second coordination sphere. The XAS spectra for truncated and full-length A{beta}-Cu{sup 2+} peptides are similar. The novel distorted six-coordinated (3N3O) geometry around copper in the A{beta}-Cu{sup ...

Energy Technology Data Exchange (ETDEWEB)

NMR spectroscopy combined with paramagnetic relaxation agents was used to study the positioning of the 40-residue Alzheimer Amyloid {beta}-peptide A{beta}(1-40) in SDS micelles. 5-Doxyl stearic acid incorporated into the micelle or Mn{sup 2+} ions in the aqueous solvent were used to determine the position of the peptide relative to the micelle geometry. In SDS solvent, the two {alpha}-helices induced in A{beta}(1-40), comprising residues 15-24, and 29-35, respectively, are surrounded by flexible unstructured regions. NMR signals from these unstructured regions are strongly attenuated in the presence of Mn{sup 2+} showing that these regions are positioned mostly outside the micelle. The central helix (residues 15-24) is significantly affected by 5-doxyl stearic acid however somewhat less for residues 16, 20, 22 and 23. This {alpha}-helix therefore resides in the SDS headgroup region with the face with residues 16, 20, 22 and 23 directed away ...

International Nuclear Information System (INIS)

Aminophenpyramine, a derivative of mepyramine (pyrilamine), a typical antagonists of histamine at its H_1 receptor was synthesized and converted into ["1"2"5I]iodoazidophenpyramine, a potential photoaffinity probe for the H_1 receptor. In the dark, reversible binding of this probe to cerebellar membranes occurred with a K/sub d/ of 1.2 x 10"-"1"1 M and a B/sub max/ of 240 fmol/mg of protein and was inhibited by various H_1-receptor antagonists with the expected potencies. These features establish the compound as one of the most potent H_1-receptor antagonists known so far. Upon IV irradiation, 5% of the bound radioactivity was covalently incorporated into cerebellar membrane polypeptides as shown by standard NaDodSO_4/PAGE. Two bands of 47 and 56 kDa were consistently labeled, labeling being prevented by various H_1-receptor antagonists with the expected potencies and stereoselectivity. In the presence of protease inhibitors, labeling of the 56-kDa peptide ...

British Library Electronic Table of Contents (United Kingdom)

Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers at various stages of development by the pollen mother cells. From this library, we identified small heat shock protein LimHSP16.45 was highly expressed during the late zygotene to pachytene stages. Our results also showed that LimHSP16.45 was almost specifically expressed in the anther compared with the root, stem, or leaf, and in situ expression of LimHSP16.45 mRNAs showed strong signals in the pollen mother cells and tapetal cells. LimHSP16.45 could be induced by heat and c...

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We aim to isolate cDNAs mapping to human chromosome 16 and localise such cDNAs on the high resolution physical map. In collaboration with LANL, PCR primers will be synthesised from cDNA sequences mapped to chromosome 16 and used as ESTs in the generation of mega-YAC contigs for this chromosome. Probing of high density cosmid grids will enable integration of the ESTs into cosmid contigs and location of the cosmid contigs on the YAC contig. A hn-cDNA library has been constructed from the hybrid CY18 which contains chromosome 16 as the only human chromosome. A modified screening protocol has been successfully developed and 15 hn-cDNA clones have been sequenced and localised on the hybrid map. Sequence analysis of four of these revealed that they were known cDNAs, which are now mapped to chromosome 16. Development of techniques to allow the isolation of longer cDNAs from the identified exons is in progress. This will depend on PCR amplification of cDNAs from a total ...

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High-throughput synthesis and screening methods have been developed for the discovery of highly active catalysts for the control of emissions from stationary and mobile sources. Low temperature CO oxidation, CO methanation, NOx abatement and the destruction of Volatile Organic Compounds (VOCs) will be discussed. The discovery libraries for primary screening consisted of both 11x11 and 16x16 catalyst arrays on 3 inch and 4 inch quartz wafers, respectively. Catalysts were prepared by robotic liquid dispensing techniques and screened for catalytic activity in Symyx's Scanning Mass Spectrometer. The screening protocols encompassed mixed metal oxides, perovskites and supported base and noble metals. Active hits were further optimized in focus libraries using shallower compositional gradients. The ScanMS is a fast serial screening tool that uses flat wafer catalyst surfaces, local laser heating, a scanning/sniffing nozzle and a quadrupolar ...

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Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in situ hybridization of a ...

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pD10 is a 0.85 kb fragment with BamHI ends cloned in pSP64. It was sub-cloned from {lambda}D10 which was isolated from a genomic library constructed in {lambda}EMBL3 from a rodent human somatic cell hybrid. The probe was localized to 19q13.2-19qter on a panel of rodent human somatic cell hybrids. Co-dominant segregation was shown in 32 families of 280 individuals.

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The nuclear data activity at AERE, Savar is briefly presented in this paper. Major thrust is on the customization of cross section libraries for general purpose reactor and shielding calculations. The processing codes that are available are NJOY91.91, some AMPX-Modules and the modules in SCALE-PC. Recent measurements on cross section data over the energy range 13-15 MeV at the Institute of Nuclear science and Technology have been reviewed. Measurements and calculations are based on the determination of excitation functions of neutron induced reactions on the elements and isotopes of FRT-relevant structural materials. (author).

Energy Technology Data Exchange (ETDEWEB)

Recent developments in the physical model of 1 MeV to 100 TeV hadron and lepton interactions with nuclei and atoms are described. These include a new nuclear cross section library, a model for soft pion production, the cascade-exciton model, the dual parton model, deuteron-nucleus and neutrino-nucleus interaction models, detailed description of mu, pi and anti p absorption and a unified treatment of muon and charged hadron electromagnetic interactions with matter. New algorithms are implemented into the MARS13(98) Monte Carlo code and benchmarked against experimental data. The code capabilities to simulate cascades and generate a variety of results in complex media have been also enhanced.

Energy Technology Data Exchange (ETDEWEB)

The fastest rate of growth in the space conditioning market has been attributed to the application of geothermal heat pumps to the industrial and commercial markets. The author reviews and provides examples of the three most popular components of a large geothermal heat pump system: geothermal earth coupling, heat pumps and heat exchangers, and building side terminals. These components are applied to three example geothermal installations: a library, an office building, and a school. Examples of energy use before and after the installation of a geothermal space conditioning system are summarized. General design and cost guidelines and comments on the use of geothermal heat pump in these and similar applications are discussed.

British Library Electronic Table of Contents (United Kingdom)

Correct prediction of the fission products inventory in irradiated nuclear fuels is essential for accurate estimation of fuel burnup, establishing proper requirements for spent fuel transportation and storage, materials accountability and nuclear forensics. Such prediction is impossible without accurate knowledge of neutron induced fission yields. The uncertainty of the fission yields reported in the ENDF/B-VII.0 library is not uniform across all of the data and much of the improvement is desired for certain fissioning isotopes and fission products. We discuss our measurements of cumulative fission yields in nuclear fuels irradiated in thermal and fast reactor spectra using Inductively Coupled Plasma Mass Spectrometry.

CERN Document Server

The Astrophysics Data System (ADS) provides an extensive system of links between the literature and other on-line information. Recently, the journals of the American Astronomical Society (AAS) and a group of NASA data centers have collaborated to provide more links between on-line data obtained by space missions and the on-line journals. Authors can now specify which data sets they have used in their article. This information is used by the participants to provide the links between the literature and the data. The ADS is available at: http://ads.harvard.edu

Wastenet

...O | P | Q | R | S | T | U | V | W | X | Y | Z 230 Christianity: general resources ...com: Christianity: General Anno Domini: Jesus Through the Centuries Bible and Homosexuality Christian Theological Research Fellowship Papers Christianity Links and Resources Directory ...Good Digital Collections Lambert's Web Links MUNDUS New Testament Gateway Omnilist of Christian Starting Points Quartz Hill School of Theology Web Library Theology Online ...com: Christianity: General Offers original articles and features about Christianity in general plus annotated links to selected relevant Internet resources, compiled by ...

Energy Technology Data Exchange (ETDEWEB)

This compilation contains 47 ACRS reports submitted to the Commission, or to the Executive Director for Operations, during calendar year 1996. It also includes a report to the Congress on the NRC Safety Research Program. All reports have been made available to the public through the NRC Public Document Room, the U.S. Library of Congress, and the Internet at http://www.nrc.gov/ACRSACNW. The reports are divided into two groups: Part 1 contains ACRS reports by project name and by chronological order within project name. Part 2 categorizes the reports by the most appropriate generic subject area and by chronological order within subject area.

Science.gov (United States)

A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7?d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further. PMID:20715132

British Library Electronic Table of Contents (United Kingdom)

The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR ab on T cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture. The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation of the four cysteine residues involved in disulphide bonds and of some amino acids with an importan...

British Library Electronic Table of Contents (United Kingdom)

Abstract Many solute carrier transporters that interact with anticancer agents and contribute to their pharmacokinetics have been shown to be differentially upregulated in cancer cells as a result of adaptive response to altered nutritional requirements. This review focuses on pathophysiological function of membrane transporters responsible for the influx of physiological substances including oligopeptides, amino acids, and organic cations and anions, and summarizes the recent knowledge regarding mechanisms in their gene expressions. Broad substrate specificity of enhanced oligopeptide H+/peptide cotransporter 1 activity in cancer cells is useful for tumor tissue-specific delivery of chemotherapeutic agents and positron emission tomography diagnostic probes. Amino acid transporters such as...

British Library Electronic Table of Contents (United Kingdom)

Aberrant amyloid-b peptide (Ab) accumulation along with altered expression and function of nicotinic acetylcholine receptors (nAChRs) stand prominently in the etiology of Alzheimer's disease (AD). Since the discovery that Ab is bound to a7 nAChRs under many experimental settings, including post-mortem AD brain, much effort has been expended to understand the implications of this interaction in the disease milieu. This research update will review the current literature on the a7 nAChR-Ab interaction in vitro and in vivo, the functional consequences of this interaction from sub-cellular to cognitive levels, and discuss the implications these relationships might have for AD therapies.

British Library Electronic Table of Contents (United Kingdom)

Abstract Under eons of evolutionary and environmental pressure, biological systems have developed strong and lightweight peptide-based polymeric materials by using the 20 naturally occurring amino acids as principal monomeric units. These materials outperform their man-made counterparts in the following ways: 1)-multifunctionality/tunability, 2)-adaptability/stimuli-responsiveness, 3)-synthesis and processing under ambient and aqueous conditions, and 4)-recyclability and biodegradability. The universal design strategy that affords these advanced properties involves -bottom-up- synthesis and modular, hierarchical organization both within and across multiple length-scales. The field of -biomimicry--elucidating and co-opting nature-s basic material design principles and molecular building blo...

British Library Electronic Table of Contents (United Kingdom)

Background: Thyroid-stimulating autoantibodies (TSAb) bind to the thyrotropin receptor (TSHR) extracellular domain, or ectodomain (ECD), comprising a leucine-rich repeat domain (LRD) linked by a hinge region to the transmembrane domain (TMD). The LRD (residues 22???260; signal peptide 1???21) contains two disulfide-bonded loops at its N-terminus. In the crystal structure of the isolated LRD complexed with human TSAb monoclonal antibody (mAb) M22, N-terminal disulfide loop 1 (residues 22???30) could not be determined because of crystal disorder. Nevertheless, present crystal structure data are interpreted to exclude a role for the LRD N-terminal disulfide loops in the TSAb epitope(s), contradicting prior functional evidence of a role for these loops in TSAb function. Materials and Methods: ...

British Library Electronic Table of Contents (United Kingdom)

Successful electron capture dissociation (ECD) Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) applications to peptide and protein structural analysis have been enabled by constant progress in implementation of improved electron injection techniques. The rate of ECD product ion formation has been increased to match the liquid chromatography and capillary electrophoresis timescales, and ECD has been combined with infrared multiphoton dissociation in a single experimental configuration to provide simultaneous irradiation, fast switching between the two techniques, and good spatial overlap between ion, photon, and electron beams. Here we begin by describing advantages and disadvantages of the various existing electron injection techniques for ECD in FT-ICR MS. We next ...

British Library Electronic Table of Contents (United Kingdom)

Gastrointestinal patches represent a novel multiparticulate drug delivery system able to increase the intestinal absorption of drugs with poor bioavailability. The number of patches to administer is a critical issue since it is related to the surface and drug concentration at the absorption site. The objective of this article is to evaluate the effect of the number of administered patches on the final absorption of leuprolide, a peptide chosen as model drug, assuming complete adhesion of all the devices to the intestinal membrane. The same dose of leuprolide was encapsulated into 2, 4 and 6 patches; the resulting intestinal absorption profiles were measured with the Ussing chamber ex vivo experimental setup and compared between them. The results showed that varying the number of patches, t...

British Library Electronic Table of Contents (United Kingdom)

Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzy...

Energy Technology Data Exchange (ETDEWEB)

Carbohydrate and nucleotide structural determination using modern spectroscopic techniques is dependent on our ability to label oligonucleotides and oligosaccharides with stable isotopes. Uniform Carbon 13 and Nitrogen 15 labeling of oligonucleotides is important to present-day efforts, which are focused on determining the structure of relatively small oligosaccharides and oligonucleotides, which form the elements of larger structures. Because of the relatively recent interest in three-dimensional structure, the development of techniques used to label them has lagged behind parallel techniques used to label peptides and proteins. Therefore, this group`s discussion focused primarily on problems faced today in obtaining oligonucleotides labeled uniformly with carbon 13 and nitrogen 15.

British Library Electronic Table of Contents (United Kingdom)

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiatio...

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The response of Desulfovibrio vulgaris Hildenborough (DvH),a sulphate-reducing bacterium, to nitrate stress was examined usingquantitative proteomic analysis. DvH was stressed with 105 m M sodiumnitrate(NaNO3), a level that caused a 50 percent inhibition in growth.The protein profile of stressed cells was compared with that of cellsgrown in the absence of nitrate using the iTRAQ peptide labellingstrategy and tandem liquid chromatography separation coupled with massspectrometry (quadrupoletime-of-flight) detection. A total of 737 uniqueproteins were identified by two or more peptides, representing 22 percentof the total DvH proteome and spanning every functional category. Theresults indicate that this was a mild stress, as proteins involved incentral metabolism and the sulphate reduction pathway were unperturbed.Proteins involved in the nitrate reduction pathway increased. Increasesseen in transport systems for proline, glycine^ ...

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The authors have shown previously that the domain recognizing receptors on activated human platelets is located on the human fibrinogen {gamma} chain between residues 400 and 411. To study the correlation between the structure of this segment of the {gamma} chain and its reactivity toward receptors on ADP-activated human platelets, they designed a series of analogues containing replacements at 9 out of 12 positions. A double substitution of the normal His{sup 400}-His{sup 401} sequence by Ala-Ala reduced the inhibitory potency of the dodecapeptide 3-fold. When Lys{sup 406} was replaced by Arg, the inhibitory potency of the dodecapeptide decreased 15 times. On the other hand, substitution of Ala{sup 408} with Arg increased the inhibitory potency of the dodecapeptide 6-fold. A drastic decrease in the reactivity of the dodecapeptide toward platelet receptors was observed when Val{sup 411} was replaced by leucine or cysteine or tyrosine. A 3-fold decrease in reactivity was noted when ...

Energy Technology Data Exchange (ETDEWEB)

When Silk fibre derived from Bombyx mori, a native biopolymer, was dissolved in highly concentrated neutral salts such as CaCl{sub 2}, the regenerated liquid silk, a gradually degraded peptide mixture of silk fibroin, could be obtained. The silk fibroin nanoparticles were prepared rapidly from the liquid silk by using water-miscible protonic and polar aprotonic organic solvents. The nanoparticles are insoluble but well dispersed and stable in aqueous solution and are globular particles with a range of 35-125 nm in diameter by means of TEM, SEM, AFM and laser sizer. Over one half of the {epsilon}-amino groups exist around the protein nanoparticles by using a trinitrobenzenesulfonic acid (TNBS) method. Raman spectra shows the tyrosine residues on the surface of the globules are more exposed than those on native silk fibers. The crystalline polymorph and conformation transition of the silk nanoparticles from random-coil and {alpha}-helix form (Silk I) into ...

International Nuclear Information System (INIS)

The active site of chicken gizzard myosin was labeled by direct photoaffinity labeling with ["3H]UDP. ["3H] UDP was stably trapped at the active site by addition of vanadate (Vi) and Co"2"+. The extraordinary stability of the myosin.Co2+.[3H]UDP.Vi complex (t1/2 greater than 5 days at 0 degrees C) allowed it to be purified free of extraneous ["3H]UDP before irradiation began. Upon UV irradiation, greater than 60% of the trapped ["3H]UDP was photoincorporated into the active site. Only the 200-kDa heavy chain was labeled, confirming earlier results using ["3H]UTP. Extensive tryptic digestion of photolabeled myosin subfragment 1 followed by high performance liquid chromatography separations and removal of nucleotide phosphates by treatment with alkaline phosphatase allowed two labeled peptides to be isolated. Sequencing of the labeled peptides and radioactive counting showed that Glu185 was the residue labeled. Since UDP is a zero-length ...

International Nuclear Information System (INIS)

1 - Description or function: Continuous energy cross-section data library for the Monte Carlo program MCNP based on the JEF-2.2 evaluated nuclear data library (ACE Format). Format: ACE Number of groups: Continuous energy Nuclides (107): H-1, H-2, He-4, Li-6, Li-7, Be-9, B-10, B-11, C-nat, N-14, N-15, O-16, O-17, F-19, Na-23, Mg-nat, Al-27, Si-nat, Cl-nat, Ti-nat, Cr-50, Cr-52, Cr-53, Cr-54, Mn-55, Fe-54, Fe-56, Fe-57, Fe-58, Co-59, Ni-58, Ni-60, Ni-61, Ni-62, Ni-64, Zr-90, Zr-91, Zr-92, Zr-94, Zr-96, Zr-nat, Nb-93, Mo-92, Mo-94, Mo-95, Mo-96, Mo-97, Mo-98, Mo-100, Mo-nat, Tc-99, Ru-101, Ru-102, Ru-104, Rh-103, Pd-105, Pd-107, Ag-109, I-129, Xe-131, Cs-133, Pr-141, Nd-143, Nd-145, Pm-147, Sm-147, Sm-149, Sm-150, Sm-151, Sm-152, Eu-153, Gd-154, Gd-155, Gd-156, Gd-157, Gd-158, Gd-160, Hf-174, Hf-176, Hf-177, Hf-178, Hf-179, Hf-180, Pb-nat, Bi-209, Th-232, U-234, U-235, U-236, U-238, Np-237, Pu-238, Pu-239, Pu-239bis, Pu-240, Pu-241, Pu-242, ...

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This report provides a user's manual for PROTOCOL, a comprehensive coupled kinetic/equilibrium computer program for analyzing the dissolution reactions of solids with aqueous solutions, specifically applied to the potential corrosion of vitrified nuclear waste by groundwater. The capabilities and available options are summarized as well as instructions for setting up and running problems. Also described in this report and included in the PROTOCOL software package are MASTER, a master file of species thermodynamic data, MANEQL, a preprocessor program and POSTP, a postprocessor. POSTP provides offline plotting using the CRAY-1 DISSPLA 9.0 graphics library. PROTOCOL is operational on the CDC-7600 and CRAY-1 computers at the Lawrence Livermore National Laboratory. 7 references, 10 figures, 2 tables.

British Library Electronic Table of Contents (United Kingdom)

Lead discovery in the pharmaceutical environment is largely an industrial-scale process in which it is typical to screen 1-5 million compounds in a matter of weeks using High Throughput Screening (HTS). This process is a very costly endeavor. Typically a HTS campaign of 1 million compounds will cost anywhere from $500000 to $1000000. There is consequently a great deal of pressure to maximize the return on investment by finding fast and more effective ways to screen. A panacea that has emerged over the past few years to help address this issue is in silico screening. In silico screening is now incorporated in all areas of lead discovery; from target identification and library design, to hit analysis and compound profiling. However, as lead discovery has evolved over the past few years, so h...

British Library Electronic Table of Contents (United Kingdom)

Moodle is an extended learning management system for developing learning units, including mathematically-based subjects. A wide variety of material can be developed in Moodle which contains facilities for forums, questionnaires, lessons, tasks, wikis, glossaries and chats. Therefore, the Moodle platform provides a meeting point for those working in a mathematics course. Mathematics requires special materials and activities: The material must include mathematical objects and the activities included in the virtual course must be able to do mathematical computations. WIRIS is a powerful software for educational environments. It has libraries for calculus, algebra, geometry and much more. In this article, examples showing the use of WIRIS in numerical methods and examples of using a new tool, ...

British Library Electronic Table of Contents (United Kingdom)

This study deals with the neutronics analysis of the current core configuration of a 3MW TRIGA Mark-II research reactor at Atomic Energy Research Establishment (AERE), Savar, Dhaka, Bangladesh and validation of the results by benchmarking with the experimental, operational and available Safety Analysis Report (SAR) values. The comprehensive neutronics code system SRAC was used to develop a versatile and accurate full-core model of the TRIGA core. The model represents in detail all components of the core with literally no physical approximation. All fresh fuel and control elements as well as the vicinity of the core were precisely described. Cross-section data library generated from JENDL-3.2 were used. The validation of the model against benchmark experimental results is presented. The SRA...

British Library Electronic Table of Contents (United Kingdom)

Abstract Objectives: This investigation reviewed experimental studies of laser irradiation of wound healing in mice and rats published from 2003 to August 2008, respectively, to assess putative stimulatory effects of this treatment. Background: Animal models, including rodents, attempt to reflect human wound healing and associated problems such as dehiscence, ischemia, ulceration, infection, and scarring. They have played a key role in furthering understanding of underlying mechanisms involved in impaired wound healing, and in testing new therapeutic strategies including laser irradiation. Method: Original research papers investigating effects of laser or monochromatic light therapy on wound healing in mice and rats and published from January 2003 to August 2008 were retrieved from library...

Science.gov (United States)

We report for the first time a relationship between the Tpl-2/cot oncogene and Mouse Mammary Tumor Virus (MMTV) associated transformation of mammary gland cells. A sub-genomic library generated from a primary mammary gland tumor yielded a novel MMTV integration site which disrupted the Tpl-2/cot proto-oncogene between exons 7 and 8. Comparison of a cell line derived from normal mammary gland (comma-D) and a cell line established from an MMTV induced mammary tumor (GR) demonstrated similar rearrangements within Tpl-2/cot for the GR cells but not in the comma-D cells. These rearrangements in the cell line were accompanied by an increase in the level of Tpl-2/cot specific mRNA. This data suggests that Tpl-2/cot expression may be important in epithelial cell transformation or tumor progression. PMID:8934549

Energy Technology Data Exchange (ETDEWEB)

The gene encoding the human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R1) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human/rodent somatic cell hybrid mapping panel. This assignment was confirmed and the gene localized to chromosome band 7p14 by fluorescence in situ hybridization. A yeast artificial chromosome containing ADCYAP1R1 was identified in the CEPH {open_quotes}B{close_quotes} Mega-YAC library. This YAC includes two highly polymorphic dinucleotide repeat sequences that will facilitate genetic studies of the contribution of ADCYAP1R1 in disease states of the central nervous and neuroendocrine systems. 13 refs., 1 fig.

International Nuclear Information System (INIS)

Neutron cross sections for Pu-239 and Am-241, -242m, -243 have been analyzed with the aid of theoretical models. A deformed optical potential that fits total, elastic and inelastic differential cross section data and neutron strength functions for Pu-239 and Am-241 have been used. In case of Pu-239 the consistency of absolute fission data and (n, 2n) cross section is investigated. Because of the strong discrepancies in Am fission cross section data a consistent set of calculated cross section values for the chain of Am nuclei is proposed. The present state of knowledge concerning first chance fission cross section allows to analyze fission cross section data of Am-241, -242m, -243, up to 20 MeV. The results thus obtained are compared with ENDF/B-V and JENDL-3 libraries. (orig.).

British Library Electronic Table of Contents (United Kingdom)

AbstractBackground Randomised controlled trials (RCTs) in the 1970s and early 1980s showed the polysaccharide serogroup A vaccine (SgAV) prevented serogroup A meningococcal meningitis (SGAMM). Subsequent non-RCTs suggested significant variations in the age-specific duration of protection among children. Objectives To determine the protective effect, duration of protection, age-specific effects and the effect of booster doses in children of the SgAV against SGAMM. Search strategy We searched the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2010, issue 2) which contains the Acute Respiratory Infections Group's Specialised Register, MEDLINE (January 1950 to May Week 3, 2010) and EMBASE (January 1974 to May 2010). Selection criteria We included RCTs. Non-RCTs ...

Energy Technology Data Exchange (ETDEWEB)

It is planned to bulk-heat a unit cell of a fusion reactor solid-breeder blanket in a fission reactor to study thermo-mechanical and thermal-hydraulic properties of fusion blankets. This study investigates the neutronic feasibility of using the Power Burst Facility (PBF) for this purpose. Heating rates were calculated for a Li/sub 2/O experiment placed in the PBF test space. The ANISN code and a 56-group coupled neutron-gamma library based on FLUNG and VITAMIN C were used to compute the heating rates. The results show that an average heating rate level of 1-3 W/cc can be produced in PBF with a local power profile that should be typical of a fusion blanket unit cell.

Energy Technology Data Exchange (ETDEWEB)

A complete code based on the finite element method (FEM) is used to study the attenuation of gamma rays, of source energy 9 MeV, in simple homogeneous lead and water systems. The results are compared, in tables and graphs, with those produced by a Monte Carlo method of solution. In the comparison, particular attention is paid to the annihilation gamma rays generated by the pair production process, to energy deposition, and to the leakage spectra. A model, multilayer, slab shield, having a fission neutron source impinging on the left-hand face, is also solved by the FEM code, using data from a coupled neutron/gamma ray cross section library. The resulting neutron and gamma ray flux distributions, and the leakage spectra, are shown graphically. (author).

Energy Technology Data Exchange (ETDEWEB)

This compilation contains 50 ACRS reports submitted to the Commission, Executive Director for Operations, or to the Office of Nuclear Regulatory Research, during calendar year 1992. It also includes a report to the Congress on the NRC Safety Research Program. All reports have been made available to the public through the NRC Public Document Room and the US Library of Congress. The reports are divided into two groups: Part 1: ACRS Reports on Project Reviews, and Part 2: ACRS Reports on Generic Subjects. Part I contains ACRS reports alphabetized by project name and by chronological order within project name. Part 2 categorizes the reports by the most appropriate generic subject area and by chronological order within subject area.