WorldWideScience
1

Infected nonunion of the long bones.  

Science.gov (United States)

BACKGROUND: Although definitions vary, infected nonunion has been defined as a state of failure of union and persistence of infection at the fracture site for 6 to 8 months.>). Infected nonunions of the supracondylar region of the femur are uncommon and are mostly due to a severe open fracture with extensive comminution and segmental bone loss or after internal fixation of a comminuted closed fracture. Associated factors include exposed bone devoid of vascularized periosteal coverage for more than 6 weeks, purulent discharge, a positive bacteriological culture from the depth of the wound, and histologic evidence of necrotic bone containing empty lacunae. Soft-tissue loss with multiple sinuses, osteomyelitis, osteopenia, complex deformities with limb-length inequality, stiffness of the adjacent joint, polybacterial multidrug-resistant infection, and smoking all complicate treatment and recovery. Although uncommon in incidence, infected ...

2007-08-01

2

Mapping strain exerted on blood vessel walls using deuterium double-quantum-filtered MRI  

UK PubMed Central (United Kingdom)

A technique is described for displaying distinct tissue layers of large blood vessel walls as well as measuring their mechanical strain. The technique is based on deuterium double-quantum-filtered (DQF)...Full Text Available

1998-04-14

3

Strain-dependent variation in collateral circulatory function in mouse hindlimb  

UK PubMed Central (United Kingdom)

The extent (density and diameter) of the native (preexisting) collateral circulation in healthy tissues and the capacity of collaterals to enlarge/remodel in obstructive arterial disease are important...Full Text Available

2010-08-01

5

Properties of a cell-wall-defective variant of Brucella abortus of bovine origin.  

UK PubMed Central (United Kingdom)

The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing...Full Text Available

1980-08-01

6

Mechanical strain enhances survivability of collagen micronetworks in the presence of collagenase: implications for load-bearing matrix growth and stability  

UK PubMed Central (United Kingdom)

There has been great interest in understanding the methods by which collagen-based load-bearing tissue is constructed, grown and maintained in vertebrate animals. To date, the responsibility for this...Full Text Available

2009-09-13

7

DIFFERENCES IN THE MECHANICAL BEHAVIOR OF CORTICAL BONE BETWEEN COMPRESSION AND TENSION WHEN SUBJECTED TO PROGRESSIVE LOADING  

UK PubMed Central (United Kingdom)

The hierarchical arrangement of collagen and mineral into bone tissue presumabley maximizes fracture resistance with respect to the predominant strain mode in bone. Thus, the ability of cortical...Full Text Available

2009-12-01

8

Complement Evasion by Borrelia burgdorferi: Serum-Resistant Strains Promote C3b Inactivation  

UK PubMed Central (United Kingdom)

The most characteristic features of the Lyme disease pathogens, the Borrelia burgdorferi sensu lato (s.l.) group, are their ability to invade tissues and to circumvent the immune defenses...Full Text Available

2001-06-01

9

Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line.  

UK PubMed Central (United Kingdom)

The ERA strain of rabies virus was propagated in a baby hamster kidney cell line (BHK-21/C13). The viral titer was 10(1.8) tissue culture infective doses (TCID) higher than that of commercial ERA vaccine....Full Text Available

1989-10-01

10

Examination of thermally polluted water for free living amoebae and testing for their possible pathogenic properties  

Energy Technology Data Exchange (ETDEWEB)

Water and mud samples were collected from canals and rivers which were adjacent to outlets discharging warm water of 3 power plants in Berlin. Downstream samples from 1 bathing resort were also collected. Free living amoebae were isolated from 138 water and 69 mud samples. From these respectively 156 and 73 strains could be cultured and were administered intranasally to mice for pathogenicity tests. Two Acanthamoeba strains from water and 7 from mud could be reisolated from mouse brain and or lungs, although no pathological disorders could be observed. Five Naegleria strains were negative in mouse inoculation tests. Four Acanthamoeba strains which were positive in mice were cultured at + 45 degrees C; no cytopathogenic effects were observed in tissue cultures. Acanthamoeba infective for mice could also be isolated from samples at low water temperatures. Further investigations have ...

1982-05-01

11

Tissue tropism and neuroinvasion of West Nile virus do not differ for two mouse strains with different survival rates  

UK PubMed Central (United Kingdom)

West Nile virus (WNV) is a mosquito-borne flavivirus that infects the central nervous system of humans and other animals. In this study, we found that C3H/HeN (C3H) mice exhibited a higher morbidity...Full Text Available

2007-11-25

12

Inbred Strain-Specific Effects of Exercise in Wild Type and Biglycan Deficient Mice  

UK PubMed Central (United Kingdom)

Biglycan (bgn)-deficient mice (KO) have defective osteoblasts which lead to changes in the amount and quality of bone. Altered tissue strength in C57BL6/129 (B6;129) KO mice, a property which...Full Text Available

2010-04-01

13

Murine respiratory mycoplasmosis (MRM) in C57BL/6N and C3H/HeN mice: strain differences in early host responses and exacerbation by nitrogen dioxide  

Energy Technology Data Exchange (ETDEWEB)

The studies reported here used genetic differences in susceptibility of C57BL/6N and C3H/HeN mice and exacerbation of the disease by nitrogen dioxide (NO/sub 2/) as tools in assessing the role of early host responses in the pathogenesis of MRM. The two strains did not differ in susceptibility to infection, but C3H/HeN mice were more susceptible to and had increased severity of lung lesions 14 days after intranasal inoculation as determined by 50% biological endpoints and morphometric analysis of tissues. Exposure to NO/sub 2/ for 4 hours prior to exposure to infectious aerosols exacerbated murine respiratory mycoplasmosis (MRM) by 7 days after exposure in both mouse strains. NO/sub 2/ appeared to affect host lung defense mechanisms responsible for limiting mycoplasmal growth in the lungs. The NO/sub 2/ exposure concentration required for this effect varied with the genetic background of the host, the dose of mycoplasmas ...

1987-01-01

14

Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-B1  

Energy Technology Data Exchange (ETDEWEB)

Mesenchymal stem cells (MSCs) are a potential source of smooth muscle cells (SMCs) for constructing tissue-engineered vascular grafts. However, the details of how specific combinations of vascular microenvironmental factors regulate MSCs are not well understood. Previous studies have suggested that both mechanical stimulation with uniaxial cyclic strain and chemical stimulation with transforming growth factor {beta}1 (TGF-{beta}1) can induce smooth muscle markers in MSCs. In this study, we investigated the combined effects of uniaxial cyclic strain and TGF-{beta}1 stimulation on MSCs. By using a proteomic analysis, we found differential regulation of several proteins and genes, such as the up-regulation of TGF-{beta}1-induced protein ig-h3 (BGH3) protein levels by TGF-{beta}1 and up-regulation of calponin 3 protein level by cyclic strain. At the gene expression level, BGH3 was induced by TGF-{beta}1, ...

2009-10-12

15

Osteocyte death and hip fracture.  

Science.gov (United States)

The viability of osteocytes can be demonstrated in sawn decalcified sections of bone by their lactate dehydrogenase activity. In the cancellous bone of the femoral head, the proportion of lacunae containing viable osteocytes decreased from 88 +/- 7% (mean +/- SD) at 10-29 years to 58 +/- 12% (P Ultimate compressive strength did not correlate with osteocyte viability. In the femoral head there is gradual, age-related reduction in osteocyte viability that can be more pronounced in hip fracture. Osteocyte death may affect bone quality by impairing repair of fatigue damage. PMID:8275364

1993-01-01

16

Aicardi syndrome: a case report and radiologic findings; Sindrome de Aicardi: relato de caso e achados radiologicos  

Energy Technology Data Exchange (ETDEWEB)

The authors report the findings of Aicardi syndrome, a disease of unknown etiology composed of multiple spasms, chorioretinal lacunae and agenesis of the corpus callosum. They present a case of Aicardi syndrome with characteristic clinical presentation and magnetic resonance imaging findings. The disease, despite being considered rare, has characteristic imaging findings. Over the past years magnetic resonance imaging has improved its ability in demonstrating other findings besides agenesis of the corpus callosum, making the radiologist's role very important in the diagnostic suspicion of this disease. (author)

2008-07-01

17

Quantitative RT-PCR Expression Analysis of Lipodepsipeptides Synthetase and Defence-related Genes in Orange Fruit in Response to Antagonist-pathogen Interaction  

British Library Electronic Table of Contents (United Kingdom)

Abstract Strains of Pseudomonas syringae are effective in controlling postharvest diseases of citrus fruits, and antagonistic activity has been correlated with in vitro production of lipodepsipeptides. Additionally, biocontrol agents can induce a range of defence mechanisms of resistance in citrus tissue that result in a broad spectrum of metabolic modifications, such as systemic acquired resistance, induced systemic resistance and production of reactive oxygen species. The aim of this study was to evaluate the expression of syringomycin (syrB1) and syringopeptin (sypA) synthetase genes from P.syringae pv. syringae biocontrol strains in vitro on different culture media and in vivo on citrus fruits (Citrus sinensis cv. Tarocco) during the interaction with Penicillium digitatum by quantitati...

2011-01-01

18

Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat.  

Science.gov (United States)

In acute liver failure following hepatitis, toxic insults, or after major liver surgery, there is an increased bacterial translocation from the gut. This may explain some of the infectious complications seen in these conditions. To elucidate mechanisms and find possible preventive measures, we investigated the effect of rectal administration of arginine and probiotic bacteria (Lactobacillus spp.) on bacterial translocation and the extent of liver failure. Sprague-Dawley rats were used and five different Lactobacillus strains (Lb. reuteri R2LC, Lb. rhamnosus DSM 6594 (= strain 271), Lb. plantarum DSM 9843 (= strain 299v), Lb. fermentum 8704:3 (= strain 245), and Lb. reuteri (= strain 108) were administered rectally once daily for 8 days with and without 2% arginine. Acute liver injury (ALI) was induced on the eighth day by intraperitoneal injection of D-galactosamine (1.1 g/kg body ...

1997-03-01

19

Ixodes ricinus: vector of a hitherto undescribed spotted fever group agent in Switzerland.  

Science.gov (United States)

A tick/rickettsial survey in various parts of Switzerland revealed the presence of a new, hitherto undescribed spotted fever group rickettsia ("Swiss agent") in up to 11.7% of I. ricinus collected off vegetation. Infection in ticks was found to be generalized with rickettsiae developing intracellularly and occasionally also intranuclearly. As a result of massive growth in ovarial tissues, including the germinative cells, the rate of transovarial and filial infection was 100%. The "Swiss agent" appears to be nonpathogenic for guinea pigs, domestic rabbits, and Swiss mice, but in male meadow voles (Microtus pennsylvanicus) it produces a microscopically detectable infection in the tunica vaginalis. The rickettsia grows well in tissue culture systems including chick embryo fibroblast, Vero, and vole tissue cells, when inoculated via yolk sac into 5-day-old hens' eggs, it kills 100% of the embryos after 5 to 7 days. Antigenic ...

1979-12-01

20

Mammary carcinogenesis in the rat after low-dose irradiation  

International Nuclear Information System (INIS)

Carcinogenesis is now generally recognized to be an important detrimental effect in man after low dose irradiation. The carcinogenic action of ionizing radiation is clearly demonstrated after exposure to relatively high doses (in excess of 0.5 Gy) but for lower dose levels reliable direct observations are not available. With the introduction of special protocols for the performance of large scale screening programs by mammography the average absorbed dose in glandular tissue in the average breast can be restricted to a value of 3 mGy per mammogram. The risk for tumor induction after exposure to these low doses can never be established from human epidemiology. In order to investigate the risk of repeated exposure to small dose of ionizing radiation the induction of mammary tumors has been investigated in rats of the WAG/Rij strain after single and fractionated irradiations with gamma-rays. (author). 8 refs., 3 figs.

1992-07-12

22

Molecular characterization of a Chinese variant of the Flury-LEP strain  

UK PubMed Central (United Kingdom)

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was...Full Text Available

23

Interrelationships in trace-element metabolism in metal toxicities in a cobalt-resistant strain of Neurospora crassa  

UK PubMed Central (United Kingdom)

A strain of Neurospora crassa was isolated by training the mould to grow on media containing high concentrations of Co2+. This strain, the CoR strain, exhibited...Full Text Available

1973-04-01

24

Applied and fundamental aspects of plant cell, tissue, and organ culture  

CERN Document Server

Applied and fundamental aspects of plant cell, tissue, and organ culture

1977-01-01

25

In Vitro Fracture of Human Cortical Bone: Local Fracture Criteria and Toughening Mechanisms  

Energy Technology Data Exchange (ETDEWEB)

A micro-mechanistic understanding of bone fracture that encompasses how cracks interact with the underlying microstructure and defines their local failure mode is lacking, despite extensive research on the response of bone to a variety of factors like aging, loading, and/or disease. Micro-mechanical models for fracture incorporating such local failure criteria have been widely developed for metallic and ceramic materials systems; however, few such deliberations have been undertaken for the fracture of bone. In fact, although the fracture event in mineralized tissues such as bone is commonly believed to be locally strain controlled, until recently there has been little experimental evidence to support this widely held belief. In the present study, a series of in vitro experiments involving a double-notch bend test geometry are performed in order to shed further light on the nature of the local cracking events that precede catastrophic fracture ...

2004-08-18

26

Normal osteoid tissue  

UK PubMed Central (United Kingdom)

The results of a histological study of normal osteoid tissue in man, the monkey, the dog, and the rat, using thin microtome sections of plastic-embedded undecalcified bone, are described. Osteoid tissue...Full Text Available

1972-03-01

27

FATIGUE OF BIOMATERIALS: HARD TISSUES  

UK PubMed Central (United Kingdom)

The fatigue and fracture behavior of hard tissues are topics of considerable interest today. This special group of organic materials comprises the highly mineralized and load-bearing tissues...Full Text Available

2010-09-01

28

Transient enhanced diffusion and deactivation of ion-implanted As in strained Si  

International Nuclear Information System (INIS)

First results on the effects of strain on transient enhanced diffusion and deactivation of As-implanted ultrashallow junctions are presented. A significant effect of strain on the magnitude and timescale of transient enhanced diffusion is observed, which is consistent with the stabilization of interstitial-type defects by tensile strain. Our results show no significant impact of strain on As electrical activity during the deactivation timescale accessed in this study.

2005-08-01

29

Decentralized Data Sharing of Tissue Microarrays for Investigative Research in Oncology  

UK PubMed Central (United Kingdom)

Tissue microarray technology (TMA) is a relatively new approach for efficiently and economically assessing protein and gene expression across large ensembles of tissue specimens. Tissue microarray technology...Full Text Available

31

University of Central Lancashire - Facilities  

Wastenet

...specialised laboratories for pharmaceutics, tissue culture and molecular biology Excellent Laboratory Facilities Tissue Culture Facilities Molecular Biology Laboratory ...

32

Detection and Diagnosis of Oral Neoplasia with an Optical ...  

Science.gov (United States)

... Thus, 0CM provides the potential to image epithelial tissue with the subcellular resolution needed to assess the pathologic state of tissue. ...

33

Immunological relationship between delta-hemolysins of Staphylococcus aureus and coagulase-negative strains of staphylococci.  

UK PubMed Central (United Kingdom)

Delta-hemolysin of coagulase-negative staphylococcal strains was immunologically identical to delta-hemolysin of Staphylococcus aureus (Newman) but distinct from delta-hemolysin from a canine strain...Full Text Available

1979-03-01

34

Effect of fatiguing exercise on longitudinal bone strain as related to stress fracture in humans.  

Science.gov (United States)

Muscular fatigue in the training athlete or military recruit has been hypothesized to cause increased bone strain that may contribute to the development of a stress fracture. Under normal circumstances, muscles exert a protective effect by contracting to reduce bending strains on cortical bone surfaces. In vivo strain studies in dogs show that muscle fatigue following strenuous exercise elevates bone strain and changes strain distribution. However, a similar experiment has yet to be performed in humans. The purpose of this work was to test the hypothesis in humans that strenuous fatiguing exercise causes an elevation in bone strain. It was also hypothesized that this elevation is greater in younger people than in older people due to the decline in muscle strength and endurance that normally occurs with age. To test these hypotheses, strain ...

35

Abnormalities in the microsomal oxidases of the WHO standard reference strain of Musca domestica*  

UK PubMed Central (United Kingdom)

Observations made during biochemical and toxicological studies of the housefly, in which the WHO standard reference (SR) strain was used as a standard, indicated that this strain differs from other...Full Text Available

1975-01-01

36

Strained silicon for quantum computing  

Energy Technology Data Exchange (ETDEWEB)

Strains in multivalley semiconductors can destroy the strict equivalence of the valleys that is demanded by cubic symmetry. Significant changes in the properties of a semiconductor may result. A proposed implementation of quantum computing with donor atoms in silicon would suffer from alterations of the donor wave functions caused by strains that are produced by fabrication processes. Deliberately straining the silicon to an extent that removed all but one valley from participation in the lowest donor state, would prevent further changes in the wave function by strain. The strain required can be achieved with established technology for depositing silicon on SiGe alloys. (author)

2002-03-07

37

Ultimate Compressive Strength and Nonlinear Stress-Strain ...  

Science.gov (United States)

... Accession Number : ADD421949. Title : Ultimate Compressive Strength and Nonlinear Stress-Strain Curves of Graphite/Epoxy Laminates,. ...

1976-10-12

38

Towards Resonant-State THz Laser Based on Strained p-Ge ...  

Science.gov (United States)

... charge carriers in strained p-Ge in crossed electric and magnetic fields was studied by means of measurements of spontaneous radiation intensity. ...

2006-07-01

39

Thermal and Electromigration-Induced Strains in Polycrystalline Films and Conductor Lines X-ray Microbeam Measurements and Analysis  

CERN Document Server

Thermal and Electromigration-Induced Strains in Polycrystalline Films and Conductor Lines

2006-01-01

40

Strain Rate Effects on Ultimate Strain of Copper  

Science.gov (United States)

... Figure 3a. PETN Filled Tube Specimen Detonated by Exploding Bridgewire Specimen POLYETIILLENE END-CAP "1- 901am WALL IHICKNESS ...

1979-05-01

41

Detection and Identification of Bacteria by Gas Chromatography1  

UK PubMed Central (United Kingdom)

Ether extracts of cultures of 29 strains representing 6 species of Bacillus, and of individual strains of Escherichia coli, Aerobacter aerogenes, and Pseudomonas...Full Text Available

1966-07-01

42

A critical look at the kinetic models of thermoluminescence-II. Non-first order kinetics  

Energy Technology Data Exchange (ETDEWEB)

Non-first order (FO) kinetics models are of three types; second order (SO), general order (GO) and mixed order (MO). It is shown that all three of these have constraints in their energy level schemes and their applicable parameter values. In nature such restrictions are not expected to exist. The thermoluminescence (TL) glow peaks produced by these models shift their position and change their shape as the trap occupancies change. Such characteristics are very unlike those found in samples of real materials. In these models, in general, retrapping predominates over recombination. It is shown that the quasi-equilibrium (QE) assumption implied in the derivation of the TL equation of these models is quite valid, thus disproving earlier workers' conclusion that QE cannot be held under retrapping dominant conditions. However notwithstanding their validity, they suffer from the shortcomings as stated above and have certain lacunae. For example, the kinetic order ...

2005-01-07

44

Magnetic forming studies  

Science.gov (United States)

Elastodynamic approach to measuring stress-strain relationship of uniaxially stressed wire, and

1967-01-01

45

Invasiveness and persistence of Salmonella enteritidis, Salmonella typhimurium, and a genetically defined S. enteritidis aroA strain in young chickens.  

UK PubMed Central (United Kingdom)

Newly hatched chicks were dosed orally with a Salmonella typhimurium wild-type strain, an S. enteritidis wild-type strain, and a genetically defined S. enteritidis aroA vaccine candidate, strain CVL30....Full Text Available

1994-11-01

46

A STUDY TO DETERMINE THE DEFORMATION ...  

Science.gov (United States)

... crystals and polycrystals. Nevertheless, explosive forming, magnetic forming, etc., are all high-strain-rate processes that ...

1963-04-01

47

The elastic plastic behaviour of a 1/2% Cr Mo V steam turbine steel during high strain thermal fatigue  

International Nuclear Information System (INIS)

High strain fatigue problem in steam turbine. Cyclic stress strain hysteresis loops and stress relaxation behaviour in 16 h dwell period tests. Variation of stress and strain during tests under nominally strain controlled conditions. Definition of test conditions and of criteria for crack initiation and failure. Comparison of reverse bend and push pull failure data. (orig.) 891 RW/orig. 892 RKD.

48

Stress analysis of hydride bed vessels used for tritium storage  

Energy Technology Data Exchange (ETDEWEB)

A prototype hydride storage bed, using LaNi{sub 4.25}Al{sub 0.75} as the storage material, was fitted with strain gages to measure strains occurring in the stainless steel bed vessel caused by expansion of the storage powder upon uptake of hydrogen. The strain remained low in the bed as hydrogen was added, up to a bed loading of about 0.5 hydrogen to metal atom ratio (H/M). The strain then increased with increasing hydrogen loading ({approximately} 0.8 H/M). Different locations exhibited greatly different levels of maximum strain. In no case was the design stress of the vessel exceeded.

1991-01-01

49

Strain softening and hardening effects in Ti-6Al-4V during high temperature deformation  

International Nuclear Information System (INIS)

Effect of strain and temperature on high temperature deformation properties of an alpha + beta titanium alloy Ti-6Al-4V has been investigated in the temperature range 800 to 910 deg. C by using the method of cross head speed cycling. On the basis of flow stress strain rate-strain rate sensitivity data and microstructural studies of the undeformed and deformed regions of tensile test pieces, both strain introduced hardening and softening effects were observed during the course of deformation which could be associated with grain coarsening and refining respectively. (author)

50

The direct comparison of health and ulcerated stomach tissue: A multiple probe microdialysis sampling approach  

UK PubMed Central (United Kingdom)

The ability to directly compare gastric ulcerated and healthy tissue would aid in the understanding of the physiological differences between these tissue types. Presently, these comparisons...Full Text Available

2008-09-10

51

Proteomic patterns analysis with multivariate calculations as a promising tool for prompt differentiation of early stage lung tissue with cancer and unchanged tissue material  

UK PubMed Central (United Kingdom)

BackgroundLung cancer diagnosis in tissue material with commonly used histological techniques is sometimes inconvenient and in a number of cases leads to ambiguous conclusions. Frequently...Full Text Available

52

Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma  

Science.gov (United States)

... protein is abundant in cytosolic portions of malignant epithelial tissue (Fig. 3A). In contrast, the degree of CCTβ staining in normal epithelial tissue (Fig. 3B) or connective tissues was much weaker ......

53

Advances in cancer tissue microarray technology: Towards improved understanding and diagnostics  

UK PubMed Central (United Kingdom)

Over the past few years, tissue microarray (TMA) technology has been established as a standard method for assessing the expression of proteins or genes across large sets of tissue specimens....Full Text Available

2006-03-30

54

A Morphological and Immunohistochemical Comparison of Mammary Tissues from the Short-Tailed Fruit Bat (Carollia ...  

Science.gov (United States)

... on the presence of leukocytes in the mammary epithelial tissue [11], temporal differentiation of the epithelial cells during ... of the gland and were separated from the epithelial tissue by bundles of co...

55

Confocal Imaging of Biological Tissues Using Second Harmonic Generation  

Energy Technology Data Exchange (ETDEWEB)

A confocal microscopy imaging system was devised to selectively detect Second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

2000-03-06

56

Sudden infant death syndrome and placental disorders: the thyroid-selenium link.  

Science.gov (United States)

Placental insufficiency, inducing hypoxia-ischaemia, is considered a major cause of neuronal injury and impaired post natal development. Placental insufficiency alters the metabolism of arachidonic acid and its oxidation products. Premature labour and low-birth-weight infants are associated with reduced intrauterine blood-flow and infections of the reproductive tract. Thyroidal activity is depressed in undernutrition (placental insufficiency). Premature infants require extra vitamin C for normal tyrosine metabolism (tyrosine is the thyroxine precursor). Among the symptoms indicating infantile cretinism, which appear during 3-5 months of age are: delayed union of skull bones, torpid behaviour, slow feeding, cyanosis during feeding, excessive sleepiness, enlarged tongue, umbilical herniation, flabby musculature, short stature and delayed development. These symptoms have all been described in low-birth-weight infants and sudden infant death syndrome victims by various authors. Bacteria ...

1997-04-01

57

Impacts of additive uniaxial strain on hole mobility in bulk Si and strained-Si p-MOSFETs  

International Nuclear Information System (INIS)

Hole mobility changes under uniaxial and combinational stress in different directions are characterized and analyzed by applying additive mechanical uniaxial stress to bulk Si and SiGe-virtual-substrate-induced strained-Si (s-Si) p-MOSFETs (metal-oxide-semiconductor field-effect transistors) along (110) and (100) channel directions. In bulk Si, a mobility enhancement peak is found under uniaxial compressive strain in the low vertical field. The combination of (100) direction uniaxial tensile strain and substrate-induced biaxial tensile strain provides a higher mobility relative to the (110) direction, opposite to the situation in bulk Si. But the combinational strain experiences a gain loss at high field, which means that uniaxial compressive strain may still be a better choice. The mobility enhancement of SiGe-induced strained p-MOSFETs ...

2009-10-01

58

Non-opaque soft tissue foreign body: sonographic findings  

UK PubMed Central (United Kingdom)

BackgroundSoft tissue foreign bodies are a common cause of orthopedic consultation in emergency departments. It is difficult to confirm their existence because conventional radiology...Full Text Available

59

Neural Tissues from the Implanted Stem Cells  

International Science & Technology Center (ISTC)

Morphological, Electrophysiological and Behavioral Investigations of the Nervous Tissue Developed from the Embryonic Matrix Zone Cells of the Dorsolateral Walls of Lateral Ventricles, Implanted into the Lesioned Regions of the Adult Rat's Brain

60

Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces  

UK PubMed Central (United Kingdom)

BackgroundOsseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility...Full Text Available

61

Advanced synchronous luminescence system  

Energy Technology Data Exchange (ETDEWEB)

A method and apparatus for determining the condition of tissue or otherwise making chemical identifications includes exposing the sample to a light source, and using a synchronous luminescence system to produce a spectrum that can be analyzed for tissue condition.

1997-01-01

62

[Destruction of radioactive particles by strains of Cladosporium cladosporoides (FRES.) de Vries].  

Science.gov (United States)

Reactions on the ionizing radiation of 14 Cladosporium cladosporioides strains were studied. Only 5 of them displayed radiotropizm. The ability of C. cladosporioides strains 4 and 5 with positive radiotropizm and museum C. cladosporioides strain 396 and its alb-mutant SM without positive radiotropizm to destruct radioactive particles of Chernobyl and explosion origin was studied. Two ways of radioactive particles destruction by C. cladosporioides were established, one of them is a direct way by fungal overgrowth of hot particles and the second one an indirect way only by fungal metabolites. Mycelium of the studied C. cladosporioides strains sorbed radionuclides from radioactive particles during cultivation on the liquid and agarized media. No certain inclinations of the individual strains to accumulation of radionuclides 137Cs or 152Eu were ascertained. PMID:12664550

63

Screening for marine nanoplanktic microalgae from Greek coastal lagoons (Ionian Sea) for use in mariculture  

British Library Electronic Table of Contents (United Kingdom)

Mediterranean mariculture uses imported strains of marine phytoplankton, raising questions of ecological risk and ability to adapt to local conditions for mass culture outdoors. In this context, we report here on the mass-culture potential and chemical composition of six strains of Prasinophyceae (five strains of Tetraselmis sp. and one Pyramimonas sp.) isolated from a Greek coastal lagoon. Proximate composition had a pattern of 10?20% ash, 35?65% protein, 6?10% lipids, and 25?45% other organics including carbohydrates. The amino acid profiles were typical for the marine representatives of the class. All strains had a high PUFA content with dominant the ?3 fraction in four of them. The fatty acid profiles indicated a Tetraselmis strain with high EPA (14%) and a Pyramimonas strain with high...

2009-01-01

64

Oxidation of Elemental Sulfur by Fusarium solani Strain THIF01 Harboring Endobacterium Bradyrhizobium sp.  

British Library Electronic Table of Contents (United Kingdom)

Nineteen fungal strains having an ability to oxidize elemental sulfur in mineral salts medium were isolated from deteriorated sandstones of Angkor monuments. These fungi formed clearing zone on agar medium supplemented with powder sulfur due to the dissolution of sulfur. Representative of the isolates, strain THIF01, was identified as Fusarium solani on the basis of morphological characteristics and phylogenetic analyses. PCR amplification targeting 16S rRNA gene and analyses of full 16S rRNA gene sequence indicated strain THIF01 harbors an endobacterium Bradyrhizobium sp.; however, involvement of the bacterium in the sulfur oxidation is still unclear. Strain THIF01 oxidized elemental sulfur to thiosulfate and then sulfate. Germination of the spores of strain THIF01 was observed in a liqui...

2010-01-01

65

Antimicrobial Edible Apple Films Inactivate Antibiotic Resistant and Susceptible-Campylobacter jejuni-Strains on Chicken Breast  

British Library Electronic Table of Contents (United Kingdom)

Abstract:- Campylobacter jejuni-is the leading cause of bacterial diarrheal illness worldwide. Many strains are now becoming multidrug resistant. Apple-based edible films containing carvacrol and cinnamaldehyde were evaluated for bactericidal activity against antibiotic resistant and susceptible-C. jejuni-strains on chicken. Retail chicken breast samples inoculated with D28a and H2a (resistant strains) and A24a (a sensitive strain) were wrapped in apple films containing cinnamaldehyde or carvacrol at 0.5%, 1.5%, and 3% concentrations, and then incubated at 4 or 23 C for 72 h. Immediately after wrapping and at 72 h, samples were plated for enumeration of viable-C. jejuni. The antimicrobial films exhibited dose- and temperature-dependent bactericidal activity against all strains. Films with ...

2011-01-01

66

Viscoelasticity of various gel films prepared from solvent-soluble constituents in coal; Sekitanchu no yobai kayoseibun kara sakuseishita shushu no gel maku no nendansei  

Energy Technology Data Exchange (ETDEWEB)

Viscoelasticity of gel films prepared from solvent-soluble constituents without ash of coal using the mixed solvent of carbon disulfide and N-methyl-2-pyrrolidinone (CS2-NMP) was measured to study the network structure of the constituents. In experiment, Upper Freeport coal and Zao Zhuang coal were used as specimens. Viscoelasticity of various gels with different weight fractions of solvent was measured by creep measurement under a fixed load and stress-strain analysis under variable loads. In the 2nd and 3rd creep measurements, although no large changes in elastic strain and viscoelastic strain were found, viscous strain gradually decreased with an increase in viscosity. In the case of small weight fraction of solvent, small viscous strain and viscoelastic strain were found, while slightly large elastic strain was found. It was thus ...

1996-10-28

67

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes  

UK PubMed Central (United Kingdom)

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

68

Toxic Effects of Linear Alkylbenzene Sulfonate on Metabolic Activity, Growth Rate, and Microcolony Formation of Nitrosomonas and Nitrosospira Strains  

UK PubMed Central (United Kingdom)

Strong inhibitory effects of the anionic surfactant linear alkylbenzene sulfonate (LAS) on four strains of autotrophic ammonia-oxidizing bacteria (AOB) are reported. Two Nitrosospira...Full Text Available

2001-06-01

69

The polymorphism architecture of mouse genetic resources elucidated using genome-wide resequencing data: implications for QTL discovery and systems genetics  

UK PubMed Central (United Kingdom)

Mouse genetic resources include inbred strains, recombinant inbred lines, chromosome substitution strains, heterogeneous stocks, and the Collaborative Cross (CC). These resources were generated through...Full Text Available

2007-07-01

70

The Viral Replication Complex Is Associated with the Virulence of Newcastle Disease Virus?  

UK PubMed Central (United Kingdom)

Virulent strains of Newcastle disease virus ([NDV] also known as avian paramyxovirus type 1) can be discriminated from low-virulence strains by the presence of multiple basic amino acid residues at...Full Text Available

2010-10-01

71

Synergistic antimicrobial activity between pentacyclic triterpenoids and antibiotics against Staphylococcus aureus strains  

UK PubMed Central (United Kingdom)

BackgroundThere has been considerable effort to discover plant-derived antibacterials against methicillin-resistant strains of Staphylococcus aureus (MRSA) which...Full Text Available

72

Survival of Genetically Modified and Self-Cloned Strains of Commercial Baker's Yeast in Simulated Natural Environments: Environmental Risk Assessment  

UK PubMed Central (United Kingdom)

Although genetic engineering techniques for baker's yeast might improve the yeast's fermentation characteristics, the lack of scientific data on the survival of such strains in natural environments...Full Text Available

2005-11-01

73

Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.  

UK PubMed Central (United Kingdom)

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein...Full Text Available

1992-07-01

74

Purification and properties of penicillinases from two strains of Bacillus licheniformis: a chemical, physicochemical and physiological comparison  

UK PubMed Central (United Kingdom)

1. The penicillinases formed by penicillinase-constitutive mutant strains from two closely related varieties (749 and 6346) of Bacillus licheniformis have been isolated, characterized...Full Text Available

1965-03-01

75

Plasmid Transfer between Bacillus thuringiensis subsp. israelensis Strains in Laboratory Culture, River Water, and Dipteran Larvae  

UK PubMed Central (United Kingdom)

Plasmid transfer between strains of Bacillus thuringiensis subsp. israelensis was studied under a range of environmentally relevant laboratory conditions in vitro,...Full Text Available

2001-01-01

76

Oxygen-dependent catabolism of indole-3-acetic acid in Bradyrhizobium japonicum.  

UK PubMed Central (United Kingdom)

Some strains of Bradyrhizobium japonicum have the ability to catabolize indole-3-acetic acid (IAA). Examination of this catabolism in strain 110 by in vivo experiments has revealed an enzymatic activity...Full Text Available

1991-08-01

77

Outbreak of Infection with a Multiresistant Klebsiella pneumoniae Strain Associated with Contaminated Roll Boards in Operating Rooms  

UK PubMed Central (United Kingdom)

An outbreak with a multiresistant Klebsiella pneumoniae (MRKP) strain among seven patients admitted to the adult intensive care unit (ICU) of a regional teaching hospital in The Netherlands...Full Text Available

2005-10-01

78

New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.  

UK PubMed Central (United Kingdom)

A new strain of mouse hepatitis virus (MHV) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. Negative-contrast electron microscopy showed an abundance...Full Text Available

1979-05-01

79

Job strain and prevalence of hypertension in a biracial population of urban bus drivers.  

UK PubMed Central (United Kingdom)

OBJECTIVES. In this study we tested the association between occupational stress--as measured by job demands, decision latitude, and job strain--and hypertension in a population of 1396 Black and White...Full Text Available

1992-07-01

80

Inoculation of Scytalidium thermophilum in Button Mushroom Compost and Its Effect on Yield  

UK PubMed Central (United Kingdom)

Scytalidium thermophilum isolates in culture, as well as the endogenous strain(s) in mushroom compost, were inactivated at 70°C. This temperature was used to pasteurize composts...Full Text Available

1994-09-01

81

Increase in Internode Length of Phaseolus lunatus L. Caused by Inoculation with a Nitrate Reductase-deficient Strain of Rhizobium sp. 1  

UK PubMed Central (United Kingdom)

Dramatic differences in the height of lima beans (Phaseolus lunatus L.) treated with two different Rhizobium strains were studied. Lima beans were grown in Perlite...Full Text Available

1981-01-01

82

High temperature fatigue damage in three austenitic alloys  

Energy Technology Data Exchange (ETDEWEB)

A series of cyclic strain controlled tests have been carried out at 600/sup 0/C on three high temperature austenitic iron-based alloys, AISI type 316 stainless steel, Alloy 800 H and Sandvik 253 MA. The tests were carried out under constant total strain control using a constant strain rate of 0.005 s/sup -1/. By considering the changes in modulus throughout the life of each specimen it was found that damage evolution could be successfully predicted as a function of plastic strain range despite the fact that each alloy had been chosen because of a different stress response at 600/sup 0/C. Although each alloy accumulated fatigue damage in a similar manner the longer lives of Sandvik 253 MA and Alloy 800 H at a given total strain range were due to a smaller plastic strain component and a reduced stage I crack propagation rate. In the 253 MA alloy, slip was ...

1988-01-01

83

Genome Sequence of Campylobacter jejuni strain 327, a strain isolated from a turkey slaughterhouse  

UK PubMed Central (United Kingdom)

Campylobacter is one of the leading causes of food-borne gastroenteritis and has a high prevalence in poultry. Campylobacter jejuni subsp. jejuni 327 is a subspecies...Full Text Available

84

Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host  

UK PubMed Central (United Kingdom)

BackgroundEscherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence...Full Text Available

85

Effect of long-term caloric restriction on oxygen consumption and body temperature in two different strains of mice  

UK PubMed Central (United Kingdom)

The hypothesis, that a decrease in metabolic rate mediates the life span prolonging effect of caloric restriction (CR), was tested using two strains of mice, one of which, C57BL/6, exhibits...Full Text Available

2007-10-01

86

Deep Desulfurization of Diesel Oil and Crude Oils by a Newly Isolated Rhodococcus erythropolis Strain  

UK PubMed Central (United Kingdom)

The soil-isolated strain XP was identified as Rhodococcus erythropolis. R. erythropolis XP could efficiently desulfurize benzonaphthothiophene, a complicated model sulfur compound that...Full Text Available

2006-01-01

87

Cross protection among Haemophilus parasuis strains in immunized gnotobiotic pigs.  

UK PubMed Central (United Kingdom)

In an attempt to establish if cross protection can be induced by different strains of Haemophilus parasuis, three groups of 12 gnotobiotic pigs were immunized each with an aluminum hydroxide adsorbed...Full Text Available

1991-01-01

88

Confirmation of human Campylobacter concisus isolates misidentified as Campylobacter mucosalis and suggestions for improved differentiation between the two species.  

UK PubMed Central (United Kingdom)

A strain from human diarrhea originally identified as Campylobacter mucosalis (NCTC 12408) was examined by using 64 phenotypic characters. The similarity of this strain to 297 isolates of Campylobacter,...Full Text Available

1994-09-01

89

Comparison of geographical strains of Schistosoma mansoni in the rhesus monkey*  

UK PubMed Central (United Kingdom)

Rhesus monkeys were infected with strains of Schistosoma mansoni from Puerto Rico, St Lucia, Brazil, and Tanzania. Monkeys were killed 11-14 weeks after exposure to 600-650 cercariae....Full Text Available

1972-01-01

90

Comparison of geographical strains of Schistosoma mansoni in the mouse*  

UK PubMed Central (United Kingdom)

Mice were killed 7, 11, 19, and 27 weeks after infection with strains of Schistosoma mansoni from Puerto Rico, Brazil, St Lucia, and Tanzania. The percentage recovery of adult worms...Full Text Available

1972-01-01

91

Biomechanical strain analysis of the proximal femur following retrograde intramedullary nailing  

UK PubMed Central (United Kingdom)

BackgroundThe purpose of this study was to examine the influence of proximal retrograde intramedullary nail position on proximal femoral strain, since stress risers...Full Text Available

2010-08-12

92

Arsenic Sensing and Resistance System in the Cyanobacterium Synechocystis sp. Strain PCC 6803  

UK PubMed Central (United Kingdom)

Arsenic is one of the most important global environmental pollutants. Here we show that the cyanobacterium Synechocystis sp. strain PCC 6803 contains an arsenic and antimony resistance...Full Text Available

2003-09-01

93

Analysis of genomic differences among Clostridium botulinum type A1 strains  

UK PubMed Central (United Kingdom)

BackgroundType A1 Clostridium botulinum strains are a group of Gram-positive, spore-forming anaerobic bacteria that produce a genetically, biochemically, and biophysically...Full Text Available

94

A TRAP Transporter for Pyruvate and Other Monocarboxylate 2-Oxoacids in the Cyanobacterium Anabaena sp. Strain PCC 7120?  

UK PubMed Central (United Kingdom)

In the cyanobacterium Anabaena sp. strain PCC 7120, open reading frames (ORFs) alr3026, alr3027, and all3028 encode a tripartite ATP-independent...Full Text Available

2010-11-01

95

A New Amidohydrolase from Bordetella or Alcaligenes Strain FB188 with Similarities to Histone Deacetylases  

UK PubMed Central (United Kingdom)

The full-length gene encoding the histone deacetylase (HDAC)-like amidohydrolase (HDAH) from Bordetella or Alcaligenes (Bordetella/Alcaligenes) strain...Full Text Available

2004-04-01

96

The complexities of skeletal biology  

Science.gov (United States)

For a long time, the skeleton was seen as an amorphous tissue of little biological interest. But

2003-01-01

99

Primaquine  

Science.gov (United States)

... oxygen to the tissues in the body),nicotinamide adenine dinucleotide (NADH) deficiency (a genetic condition), glucose-6- ...

103

Determination of Shooting Distance from Deformation of the ...  

Science.gov (United States)

... Descriptors : *TERMINAL BALLISTICS, *WOUND BALLISTICS, VELOCITY, CONVERSION, REPRINTS, TISSUES(BIOLOGY), MANUFACTURING ...

106

Bone Anchored Hearing Treatment Procedure  

Medline Plus

... the top of the screen, acting as a hinge. Beneath that the soft tissue consisting of fat ...

108

The effect of temperature and flow stress for climb-controlled dislocation creep in magnesium alloy  

Energy Technology Data Exchange (ETDEWEB)

Effect of strain rate, temperature and grain size in the climb-controlled dislocation creep region for AZ31 magnesium alloy was examined at lower temperature of {proportional_to}473 K. The flow stress was not affected by the difference in grain size. The strain rate sensitivity, m, was obtained 0.14 and 0.2 at low temperatures in high strain rate and at high temperature in low strain rate, respectively. It was found that the notion of effective diffusivity could express the dominant diffusion process during climb-controlled dislocation creep behavior uniquely. (orig.)

2003-07-01

110

Strength and toughness of a metastable beta-titanium alloy as influenced by strain rate variations  

International Nuclear Information System (INIS)

Strain rate effects for the high strength, metastable beta-titanium alloy Ti-8Mo-8V-2Fe-3Al are assessed in terms of tensile and fracture toughness behavior. It is shown that as the strain rate increases the yield strength, ultimate tensile strength, and ductility also increase. As a result of the decrease in the amount of intergranular fracture as the strain rate is increased, the dynamic fracture toughness values K/sub Id/ are greater than those obtained under static conditions as given by K/sub Q/.

111

Strain induced hardening in Ti-3Al-4V at 910 deg. C  

International Nuclear Information System (INIS)

High temperature deformation behavior of an alpha + beta titanium alloy Ti-3Al-4V has been investigated at 910 deg. C. by using the method of cross head speed cycling. On the basis of flow stress, strain rate and strain rate sensitivity data, the alloy was found to exhibit strain hardening at this temperature which resulted in degradation in superplastic properties of the alloy. A high beta-phase volume fraction and grain-growth in both alpha and beta phases were considered responsible for the observed hardening effect. (author)

113

Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains?  

UK PubMed Central (United Kingdom)

Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium...Full Text Available

2011-02-01

114

ISO TC 20/SC 14 N  

Science.gov (United States)

runs are necessary, provision shall be made for expansion joints, motion of the units, or similar compensation to ensure that no excessive strains are ...

115

Experimental evaluation of angularly-variable fiber geometry for targeting depth-resolved reflectance from layered epithelial tissue phantoms  

Science.gov (United States)

The aim of the present study focuses on experimentally demonstrating the efficacy of using angularly-variable fiber geometry to achieve the desired tissue-layer selection and probing depths with the further objective of enhancing the sensitivity and specificity of spectral diagnosis in stratified architectures that resemble human cervical epithelia. The morphological and biochemical features of epithelial tissue vary in accordance with tissue depths; consequently, the accuracy of spectroscopic diagnosis of epithelial dysplasia may be enhanced by probing the optical properties of this tissue. When correlated to cellular dysplasia, layer-specific changes in tissue optical properties may be deciphered by reflectance spectroscopy coupled with angularly-variable fiber geometry. This study addresses the utility of using such angularly-variable fiber geometry for resolving ...

2007-03-01

116

Void growth and softening of a single crystal with strain gradient effects  

Energy Technology Data Exchange (ETDEWEB)

The strain gradient crystal plasticity theory is applied to study the deformation of planar single crystal with a void under a nominally uniaxial tension. The crystal theory assumes elevated strain hardening due to slip gradients and has a constitutive length scale. The effects of the void size with respect to the constitutive length scale on the single crystal deformation are investigated.

1997-02-06

117

The persistence of antibiotic resistance: evaluation of a probiotic approach using antibiotic-sensitive M. elsdenii strains to prevent colonization of swine by antibiotic-resistant strains  

Science.gov (United States)

Megasphaera elsdenii is a lactate-fermenting, obligately anaerobic bacterium commonly present in the gastrointestinal tracts of mammals, including humans. Swine M. elsdenii strains were previously shown to have high levels of tetracycline resistance (MIC = 64->256 micro g/ml) and to carry mosaic (re...

118

The Iron- and Temperature-Regulated cjrBC Genes of Shigella and Enteroinvasive Escherichia coli Strains Code for Colicin Js Uptake  

UK PubMed Central (United Kingdom)

A cosmid library of DNA from colicin Js-sensitive enteroinvasive Escherichia coli (EIEC) strain O164 was made in colicin Js-resistant strain E. coli VCS257, and colicin...Full Text Available

2001-07-01

119

Strain differences in the embryotoxicity of "2"3"9Pu  

International Nuclear Information System (INIS)

Comparison of the embryolethality of monomeric "2"3"9Pu injected at 9 days of gestation demonstrated that the sensitivity of Charles River CD strain was greater than that of Hilltop Wistar rats. Subsequent comparisons showed that rats derived from the Hilltop Fischer strain were more sensitive than the Wistar and, although not directly compared, more sensitive than the CD.

1977-05-01

120

Relationship of large and small invertases in Saccharomyces: mutant selectively deficient in small invertase.  

UK PubMed Central (United Kingdom)

A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a level...Full Text Available

1978-09-01

121

Quantifying octahedral rotations in strained perovskite oxide films.  

Energy Technology Data Exchange (ETDEWEB)

We have measured the oxygen positions in LaNiO{sub 3} films to elucidate the coupling between epitaxial strain and oxygen octahedral rotations. The oxygen positions are determined by comparing the measured and calculated intensities of half-order Bragg peaks, arising from the octahedral rotations. Combining ab initio density-functional calculations with these experimental results, we show how strain systematically modifies both bond angles and lengths in this functional perovskite oxide.

2010-07-20

122

Genetic and Structural Insights into the Dissemination Potential of the Extremely Broad-Spectrum Class A ?-Lactamase KPC-2 Identified in an Escherichia coli Strain and an Enterobacter cloacae Strain Isolated from the Same Patient in France?  

UK PubMed Central (United Kingdom)

Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum...Full Text Available

2008-10-01

123

Draft Genome Sequence of Shewanella sp. Strain HN-41, Which Produces Arsenic-Sulfide Nanotubes.  

Science.gov (United States)

The dissimilatory metal reducing bacterium Shewanella sp. strain HN-41 was first reported to produce novel photoactive As-S nanotubes via reduction of As(V) and S(2)O(3)(2-) under anaerobic conditions. Here we report the draft genome sequence and annotation of strain HN-41. PMID:21868804

2011-09-01

124

Anelastic strain recovery of amorphous metals. [80 Pd--20 Si; 80 Fe--13 P--7 C; 60 Cu--40 Zr  

Science.gov (United States)

This paper describes the anelastic strain recovery and the strain hardening of amorphous metals and presents the experimental result that creep deformation is represented by a viscoelastic model consisting of rheological elements. Materials studied were 80Pd--20Si, 80Fe--13P--7C, and 60 Cu--40 Zr. (DLC)

1976-08-01

125

Strain and Texture in Al-Interconnect Wires Measured by X-Ray Microbeam Diffraction  

Energy Technology Data Exchange (ETDEWEB)

The local strain and texture in Al interconnect wires have been investigated using white and monochromatic x-ray microbeams on the MHATTCAT undulator beam line at the Advanced Photon Source. Intergrain and intragrain orientations were obtained with ~0.01 degree sensitivity using white beam measurements on wide Al pads (~100 Mu-m) and thin (2 Mu-m) Al wires. Orientation changes of up to 1 degree were found within individual grains of the (111) textured Al interconnects. Deviatoric strain measurements indicate small intragranular strain variations, but intergranular strain variations were found to be quite large.

1999-04-05

126

Measuring back stresses in commercial alloys at elevated temperatures. [922/sup 0/K  

Energy Technology Data Exchange (ETDEWEB)

Strain transient dip tests have been conducted on two commercial heats of Alloy 800 at 922 K. Tests were conducted on both a servo-controlled hydraulic machine and a specially designed dip test machine. Using measured maximum strain changes during dips allowed unambiguous definition of a kinematic variable. The several other methods of interpreting dip test data by attempting to measure strain rates shortly after the unloading decrements were found not to be satisfactory. The nature of the instantaneous unloading strains and the strain transients following unloading are also discussed in terms of the mechanisms of deformation in Alloy 800. 9 figures, 3 tables.

1982-01-01

127

In vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens  

British Library Electronic Table of Contents (United Kingdom)

The present study describes the screening of five lactic acid bacteria (LAB) for use as probiotics based on their competitive adhesion and production of antagonistic substances against some fish pathogens. A reduction of adhesion of all pathogenic strains tested was obtained with three of the LAB strains (Lactococcus lactis subsp. lactis CLFP100, Lactococcus lactis subsp. cremoris CLFP102 and Lactobacillus curvatus CLFP150). With the exception of fish pathogens Flavobacterium psychrophilum and Renibacterium salmoninarum that were not inhibited by LAB strains, production of antagonistic compounds by all tested LAB was observed against at least one of the indicator strains. Based on mucus adhesion, competitive exclusion, and suppression of fish pathogen growth, the selected LAB strains can b...

2007-01-01

128

Antigenic characterization of phocine distemper virus causing mass mortality in 2002 and its relationship to other morbilliviruses  

British Library Electronic Table of Contents (United Kingdom)

Summary The antigenic relationship between the phocine distemper virus (PDV) strain causing the epidemic in 2002 and the PDV strain of 1988, canine distemper virus from two dogs and one marten, and one measles virus strain was investigated in vivo and in vitro using monospecific polyclonal and monoclonal antibodies directed against five different proteins of canine or phocine distemper virus (N, P, M, F, H). Epitopic mapping revealed no difference between the PDV strains causing the epidemics in 1988 or 2002. However, the use of these antibodies allowed discrimination between different morbilliviruses including a vaccine strain of canine distemper virus. The major differences among the investigated morbilliviruses were found in the H protein.

2007-01-01

129

Alloy 800: New stress rupture and creep data for pressurised components in high-temperature reactors  

International Nuclear Information System (INIS)

A new evaluation of the 1% time strain limit for creep strength and the isochronous stress/strain relationship was to be undertaken for the type 800 alloy, based on up to date experimental data. After the evaluation of the 1% time strain limit curves and time fracture curves of individual melts, multilinear regression analyses were made to clear up the relationship between parameters of the creep behaviour such as creep strength, 1% time strain limit and time fracture strain on the one hand and metallurgical parameters such as C, Ti, Al, Ni, Cr contents or grain size and type of manufacture, on the other hand. Based on the knowledge gained from these calculations, new limits were determined for HTR material specifications for variants of the 800 alloy of the Rk, NT, DE, and HT type. (orig./DG).

1988-10-06

130

Strain effect and characteristics of GaInP/AlGaInP strain-compensated multiple quantum wells  

Energy Technology Data Exchange (ETDEWEB)

Ga{sub 0.6}In{sub 0.4}P/(Al{sub 0.8}Ga{sub 0.2}){sub 0.4}In{sub 0.6}P strain-compensated multiple quantum wells (SCMQW) and Ga{sub 0.5}In{sub 0.5}P/(Al{sub 0.4}Ga{sub 0.6}){sub 0.5}In{sub 0.5}P unstrained MQW were grown by MOVPE to investigate the shift of photoluminescence (PL) peak wavelength and the carrier lifetime caused by strain effect in SCMQW. The PL peaks of SCMQW and unstrained MQW were 608 and 628 nm, respectively. From the rocking curve of SCMQW, it indicated that the active layers of SCMQW were not zero net strain. The residual strain caused the emission intensity of SCMQW to be about four times weaker than that of unstrained MQW. The carrier lifetimes of SCMQW and unstrained MQW were measured to be 3 and 6.02 ns, respectively. It was conjectured that the residual strain in SCMQW induced a decrease in the carrier lifetime by a factor of two with respect to that of ...

2003-02-15

131

Strain effect and characteristics of GaInP/AlGaInP strain-compensated multiple quantum wells  

International Nuclear Information System (INIS)

Ga_0_._6In_0_._4P/(Al_0_._8Ga_0_._2)_0_._4In_0_._6P strain-compensated multiple quantum wells (SCMQW) and Ga_0_._5In_0_._5P/(Al_0_._4Ga_0_._6)_0_._5In_0_._5P unstrained MQW were grown by MOVPE to investigate the shift of photoluminescence (PL) peak wavelength and the carrier lifetime caused by strain effect in SCMQW. The PL peaks of SCMQW and unstrained MQW were 608 and 628 nm, respectively. From the rocking curve of SCMQW, it indicated that the active layers of SCMQW were not zero net strain. The residual strain caused the emission intensity of SCMQW to be about four times weaker than that of unstrained MQW. The carrier lifetimes of SCMQW and unstrained MQW were measured to be 3 and 6.02 ns, respectively. It was conjectured that the residual strain in SCMQW induced a decrease in the carrier lifetime by a factor of two with respect to that of unstrained MQW.

2003-02-15

132

Homology among tet determinants in conjugative elements of streptococci  

Energy Technology Data Exchange (ETDEWEB)

A mutation to tetracycline sensitivity in a resistant strain of Streptococcus pneumoniae was shown by several criteria to be due to a point mutation in the conjugative o(cat-tet) element found in the chromosomes of strains derived from BM6001, a clinical strain resistant to tetracycline and chloramphenicol. Strains carrying the mutation were transformed back to tetracycline resistance with the high efficiency of a point marker by donor deoxyribonucleic acids from its ancestral strain and from nine other clinical isolates of pneumococcus and by deoxyribonucleic acids from Group D Streptococcus faecalis and Group B Streptococcus agalactiae strains that also carry conjugative tet elements in their chromosomes. It was not transformed to resistance by tet plasmid deoxyribonucleic acids from either gram-negative or gram-positive species, except for one that carried ...

1981-10-01

133

Protein expression in white spot syndrome virus infected Penaeus monodon fabricius.  

Science.gov (United States)

White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart ...

2005-06-01

134

Nature and distribution of mucosal lesions associated with enteropathogenic and enterohemorrhagic Escherichia coli in piglets and the role of plasmid-mediated factors.  

Science.gov (United States)

Bacterial attachment-effacement (att-eff) is emerging as an important virulence characteristic common to both enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). The contribution of the plasmid-encoded EPEC adherence factor to the production of mucosal lesions and diarrhea was investigated in gnotobiotic piglets. Bacterial att-aff in the intestinal mucosa of piglets infected with plasmid-cured EPEC strain E2348/69 (O127) was indistinguishable from that in piglets infected with the parent strain, but the distribution of lesions was different; it occurred in the small intestines of 6 of 7 piglets infected with the parent strain compared with only 2 of 11 (P = 0.006) infected with the plasmid-cured strain. Plasmid-encoded factors in EPEC and EHEC strains did not appear to contribute to bacterial competition with normal gut microflora. Of 13 ...

1989-04-01

135

Wound ballistics of gunshot injuries to the head and neck.  

Science.gov (United States)

It is important for the trauma surgeon to understand the basic principles of terminal gunshot ballistics and the study of the projectile's effect on striking soft tissue. The amount of kinetic energy dissipated to the tissue is directly related to the mass and velocity of the projectile as follows: K = MV2/2. Doubling the velocity quadruples the energy, while doubling the mass only doubles the energy. A temporary tissue cavity is produced as the striking projectile compresses the surrounding tissue; the higher the energy release, the more extensive the inapparent compressive damage. The permanent cavity that remains is the result of extrusion of tissue from the pathway of the projectile. The higher the velocity, the higher the likelihood of extensive damage. If the missile expands or fragments within the tissues, more damage will occur. PMID:6847483

1983-05-01

136

Uptake and Intracellular Activity of Moxifloxacin in Human Neutrophils and Tissue-Cultured Epithelial Cells  

UK PubMed Central (United Kingdom)

The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At...Full Text Available

1999-01-01

137

Tissue distribution of brain-thymus shared antigens recognized by anti-brain xenosera in the rat, dog and man.  

UK PubMed Central (United Kingdom)

A comparative and quantitative study of the tissue distribution of brain-thymus shared antigens was carried out using rabbit antisera to rat, dog and human brain homogenates, assayed on rat, dog and...Full Text Available

1979-03-01

138

Timing of Births and Reproductive Success in Captive Red-tailed Phascogales, Phascogale calura  

Science.gov (United States)

... and sperm showed an association with the oviduct epithelial tissue. Estimate of sperm storage duration At ASDP in ... Sperm heads show no particular association with the epithelial tissue. enlarge fig...

139

The influence of tethered epidermal growth factor on connective tissue progenitor colony formation  

UK PubMed Central (United Kingdom)

Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial...Full Text Available

2009-09-01

140

The Hippo tumor-suppressor pathway regulates apical-domain size in parallel to tissue growth  

UK PubMed Central (United Kingdom)

SummaryThe Hippo tumor-suppressor pathway controls tissue growth in Drosophila and mammals by regulating cell proliferation and apoptosis. The Hippo pathway includes...Full Text Available

2009-07-15

141

Sugar Efflux from Maize (Zea mays L.) Pedicel Tissue 1  

UK PubMed Central (United Kingdom)

Sugar release from the pedicel tissue of maize (Zea mays L.) kernels was studied by removing the distal portion of the kernel and the lower endosperm, followed by replacement of the...Full Text Available

1985-03-01

142

Sucrose Uptake by Sugar Beet Tap Root Tissue 1  

UK PubMed Central (United Kingdom)

Sucrose uptake by discs of mature sugar beet root tissue incubated in [14C]-sucrose exhibited nonsaturating kinetics over the concentration range of 1 to 500 millimolar. Uptake was inhibited...Full Text Available

1979-11-01

143

Should we clone human beings? Cloning as a source of tissue for transplantation.  

UK PubMed Central (United Kingdom)

The most publicly justifiable application of human cloning, if there is one at all, is to provide self-compatible cells or tissues for medical use, especially transplantation. Some have argued that...Full Text Available

1999-04-01

144

Role of serum carrier proteins in the peripheral metabolism and tissue distribution of thyroid hormones in familial dysalbuminemic hyperthyroxinemia and congenital elevation of thyroxine-binding globulin.  

UK PubMed Central (United Kingdom)

To investigate the role of thyroxine-binding globulin (TBG) and albumin in the availability of thyroid hormones to peripheral tissues, comprehensive kinetic studies of thyroxine (T4) and triiodothyronine...Full Text Available

1987-08-01

145

Relationship of pharmacokinetics and drug distribution in tissue to increased safety of amphotericin B colloidal dispersion in dogs.  

UK PubMed Central (United Kingdom)

The safety, pharmacokinetics, and distribution in tissue of an amphotericin B (AmB)-cholesteryl sulfate colloidal dispersion (ABCD) were compared with those of micellar amphotericin B-deoxycholate (m-AmB)....Full Text Available

1992-02-01

146

Relationship between Hydroxy Fatty Acids and Prostaglandin E2 in Gingival Tissue  

UK PubMed Central (United Kingdom)

Bacterial hydroxy fatty acids and alpha-hydroxy fatty acids have been demonstrated in complex lipid extracts of subgingival plaque and gingival tissue. However, little is known about the relationship...Full Text Available

1998-12-01

147

Regulation of Embryo Dormancy by Manipulation of Abscisic Acid in Kernels and Associated Cob Tissue of Zea mays L. Cultured in Vitro1  

UK PubMed Central (United Kingdom)

Sectors of Zea mays cobs, with and without kernels were cultured in vitro in the presence and absence of fluridone. Cultured kernels, cob tissue, and embryos developed...Full Text Available

1989-09-01

148

Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division.  

UK PubMed Central (United Kingdom)

Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol...Full Text Available

1990-02-01

149

Properties of rare-gas solids. [Diffusion rate of xenon through epithelial tissue  

Science.gov (United States)

Results are reported from studies of the diffusion rate of xenon through frog abdominal skin and toad urinary bladders, typical epithelial membranes. It was concluded that intracellular water serves as the principal barrier for xenon passage through epithelial tissue.

1977-01-01

150

Pathology of tissue loss (white syndrome) in Acropora sp. corals from the Central Pacific.  

Science.gov (United States)

We performed histological examination of 69 samples of Acropora sp. manifesting different types of tissue loss (Acropora White Syndrome-AWS) from Hawaii, Johnston Atoll and American Samoa between 2002 and 2006. Gross lesions of tissue loss were observed and classified as diffuse acute, diffuse subacute, and focal to multifocal acute to subacute. Corals with acute tissue loss manifested microscopic evidence of necrosis sometimes associated with ciliates, helminths, fungi, algae, sponges, or cyanobacteria whereas those with subacute tissue loss manifested mainly wound repair. Gross lesions of AWS have multiple different changes at the microscopic level some of which involve various microorganisms and metazoa. Elucidating this disease will require, among other things, monitoring lesions over time to determine the pathogenesis of AWS and the potential role of tissue-associated ...

2011-03-30

151

PREVALENCE OF MYCOPLASMA AGASSIZII AND CHELONIAN HERPESVIRUS IN CAPTIVE TORTOISES (TESTUDO SP.) IN THE UNITED KINGDOM  

Science.gov (United States)

... the tropism of both mycoplasmas and herpesviruses for epithelial tissue still indicate oral swabs to be the most ... feces.18 Alphaherpesviruses show a marked tropism for epithelial tissue, with viral rep...

152

Nurses' knowledge, attitudes, and beliefs regarding organ and tissue donation and transplantation.  

UK PubMed Central (United Kingdom)

The acute shortage of human organs and tissues for transplantation has been attributed in part to health professionals, including nurses, for their reluctance to recognize and refer suitable candidates...Full Text Available

1991-03-01

153

New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure  

UK PubMed Central (United Kingdom)

Adipose tissue is central to the regulation of energy balance. Two functionally different types of fat are present in mammals: white adipose tissue (WAT), the primary site of triglyceride storage,...Full Text Available

2008-08-21

154

Near-infrared spectroscopy technique to evaluate the effects of red blood cell transfusion on tissue oxygenation  

UK PubMed Central (United Kingdom)

IntroductionThe aim of this study was to evaluate the effects of red blood cell (RBC) transfusions on muscle tissue oxygenation, oxygen metabolism and microvascular reactivity in...Full Text Available

2009-01-01

155

Modulation of Aire regulates the expression of tissue-restricted antigens  

UK PubMed Central (United Kingdom)

AbstractIntrathymic expression of tissue-restricted antigens (TRAs) has been viewed as the key element in the induction of central tolerance and recently, a central role for the autoimmune...Full Text Available

2008-01-01

156

Mesenchymal Stem Cells for Craniofacial Tissue Regeneration: Designing Hydrogel Delivery Vehicles  

UK PubMed Central (United Kingdom)

Craniofacial injuries require a variety of different cell types to repopulate areas of bone, cartilage, tendon, and fat. Mesenchymal stem cells (MSCs) provide a multipotent cell source for tissue...Full Text Available

2009-08-01

157

Mapping the cellular and molecular heterogeneity of normal and malignant breast tissues and cultured cell lines  

UK PubMed Central (United Kingdom)

IntroductionNormal and neoplastic breast tissues are comprised of heterogeneous populations of epithelial cells exhibiting various degrees of maturation and differentiation. While...Full Text Available

2010-01-01

158

METABOLIC EFFECTS OF ACUTE EXPOSURE TO METHOPRENE IN THE AMERICAN LOBSTER, HOMARUS AMERICANUS  

Science.gov (United States)

... hepatopancreas (1.55 ppm), gonad (5.18 ppm), epithelial tissue (6.17 ppm) and, most significantly, the eyestalks ( ... by Day 1 Stage I larvae and by epithelial tissue of postmolt juvenile lobsters. Postmo...

159

Isolation of a bacterium resembling Pirellula species from primary tissue culture of the giant tiger prawn (Penaeus monodon).  

UK PubMed Central (United Kingdom)

During attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (Penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin...Full Text Available

1991-11-01

160

Increased Production and Expression of Tissue Thromboplastin-Like Procoagulant Activity In Vitro by Allogeneically Stimulated Human Leukocytes  

UK PubMed Central (United Kingdom)

Intravascular coagulation, thrombosis, and fibrin deposition often produce tissue damage in allogeneic inflammatory reactions such as allograft rejection. The mechanisms which initiate blood clotting...Full Text Available

1978-09-01

161

Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture  

UK PubMed Central (United Kingdom)

During a study on the mTor pathway in the rat kidney we observed a striking increase of the phosphorylation of the S6 kinase in mitosis. In cryostat sections of perfusion-fixed tissue mitotic cells...Full Text Available

2007-02-01

162

Identification of immune genes and proteins involved in the response of bovine mammary tissue to Staphylococcus aureus infection  

UK PubMed Central (United Kingdom)

BackgroundMastitis in dairy cattle results from infection of mammary tissue by a range of micro-organisms but principally coliform bacteria and Gram positive bacteria such as Staphylococcus...Full Text Available

163

Four year experience of sarcoma of soft tissues and bones in a tertiary care hospital and review of literature  

UK PubMed Central (United Kingdom)

BackgroundSarcoma encompasses an uncommon group of cancer and the data is insufficient from Pakistan. We report our four years experience of Sarcoma of soft tissues and bones.MethodsThis...Full Text Available

164

Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens-Mediated Transformation of Hybrid Aspen and Poplar Clones  

UK PubMed Central (United Kingdom)

Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures...Full Text Available

1990-07-01

165

Embryonic anti-aging niche  

UK PubMed Central (United Kingdom)

Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. The poor performance of stem cells in an aged...Full Text Available

166

Effects of the Developmental State of the Tissue on the Competence for Flower Bud Regeneration in Pedicel Explants of Tobacco  

UK PubMed Central (United Kingdom)

The competence of pedicel explants of tobacco (Nicotiana tabacum L. cv Samsun) to regenerate flower buds in response to auxin was manipulated by preincubating excised tissues in the...Full Text Available

1990-03-01

167

Effect of misonidazole pretreatment on nitrogen mustard-induced DNA cross-linking in mouse tissues in vivo.  

UK PubMed Central (United Kingdom)

In the present study we have used the alkaline elution technique to study the effect of misonidazole (MISO) on the initial amount of DNA cross-linking in various normal and neoplastic tissues of C3H...Full Text Available

1984-12-01

168

Development of compression-controlled low-level laser probe system: towards clinical application  

UK PubMed Central (United Kingdom)

Various physico-chemical tissue optical clearing (TOC) methods have been suggested to maximize photon density in tissue. In order to enhance photon density, a compression-controlled low-level...Full Text Available

2010-09-01

169

Determinations of organ or tissue doses to survivors in Hiroshima and Nagasaki  

International Nuclear Information System (INIS)

For the purpose of risk estimates from radiation carcinogenesis including leukemogenesis and radiation genetic effects, the biological significant dose is not the tissue kerma in air but the absorbed dose in organ or tissue with respect to carcinogenic and leukemogenic effects or genetic effects. In order to estimate organ or tissue dose from the tissue kerma in air, a ratio of the organ or tissue dose to the tissue kerma in air for survivors in Hiroshima and Nagasaki was calculated with the aid of the Snyder's mathematical phantoms constructed so as to simulate the body shape of survivors aged 5, 10 years old and adult at the time of atomic bomb detonations. The ratios were corrected for the angular distribution of atomic bomb radiations, assuming an anisotropic angular distribution for the survivors exposed to the atomic bombs in open air and the isotropic ...

1980-01-01

170

Detection and Toxin Typing of Clostridium perfringens in Formalin-Fixed, Paraffin-Embedded Tissue Samples by PCR?  

UK PubMed Central (United Kingdom)

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding...Full Text Available

2009-03-01

171

Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during lipopolysaccharide-induced acute inflammation  

UK PubMed Central (United Kingdom)

The dental pulp consists of loose connective tissue encased in rigid dentinal walls. Because of its topography the tissue has low interstitial compliance and limited capacity to expand during fluid...Full Text Available

2006-05-15

172

Current state of methodology on hemoglobin oximetry in tissues.  

Science.gov (United States)

Photon migration provides sensitive tissue oximetry in which the optical pathlength is known. Phase array gives the precise location of a subnanomolar amount of hidden absorber in highly scattering medium. PMID:8079713

1994-01-01

173

Chest Wall Resection for Adult Soft Tissue Sarcomas and Chondrosarcomas: Analysis of Prognostic Factors  

UK PubMed Central (United Kingdom)

BackgroundWide resection with tumor-free margins is necessary in soft-tissue sarcomas to minimize local recurrence and to contribute to long-term survival. Information about treatment...Full Text Available

2011-01-01

174

Adipose Tissue Gene Expression of Factors Related to Lipid Processing in Obesity  

UK PubMed Central (United Kingdom)

BackgroundAdipose tissue lipid storage and processing capacity can be a key factor for obesity-related metabolic disorders such as insulin resistance and diabetes. Lipid uptake is...Full Text Available

175

Activation of PPAR? induces profound multilocularization of adipocytes in adult mouse white adipose tissues  

UK PubMed Central (United Kingdom)

We sought to determine the effects of activation of peroxisome proliferator-activated receptor-γ (PPAR-γ) on multilocularization of adipocytes in adult white adipose tissue (WAT). Male...Full Text Available

2009-12-31

176

Aberrant repair and fibrosis development in skeletal muscle  

UK PubMed Central (United Kingdom)

The repair process of damaged tissue involves the coordinated activities of several cell types in response to local and systemic signals. Following acute tissue injury, infiltrating inflammatory cells...Full Text Available

177

A caGRID-ENABLED, LEARNING BASED IMAGE SEGMENTATION METHOD FOR HISTOPATHOLOGY SPECIMENS  

UK PubMed Central (United Kingdom)

Accurate segmentation of tissue microarrays is a challenging topic because of some of the similarities exhibited by normal tissue and tumor regions. Processing speed is another consideration...Full Text Available

2009-07-01

178

3D Hepatic Cultures Simultaneously Maintain Primary Hepatocyte and Liver Sinusoidal Endothelial Cell Phenotypes  

UK PubMed Central (United Kingdom)

Developing in vitro engineered hepatic tissues that exhibit stable phenotype is a major challenge in the field of hepatic tissue engineering. However, the rapid dedifferentiation of...Full Text Available

179

Use of integrin-linked kinase to extend function of encapsulated pancreatic tissue  

Energy Technology Data Exchange (ETDEWEB)

We have studied the impact of overexpression of an intracellular signaling protein, integrin-linked kinase (ILK), on the survival and function of encapsulated islet tissue used for the treatment of type 1 diabetes. The dimensions of the encapsulated tissue can impact the stresses placed on the tissue and ILK overexpression shows the ability to extend function of dissociated cells as well as intact islets. These results suggest that lost cell-extracellular matrix interactions in cell encapsulation systems can lead to decreased insulin secretion and ILK signaling is a target to overcome this phenomenon. (communication)

2010-12-15

181

Multiscale Mechanical Characterization of Biomimetic Gels for ...  

Science.gov (United States)

... MECHANICAL PROPERTIES, *COPOLYMERS, *SOFT TISSUES, *GELS, *BIOMIMETICS, ISOPRENE, WOUND BALLISTICS, STYRENE PLASTICS ...

2006-11-01

184

Detection of Mitochondrial DNA Mutations in Mammary ...  

Science.gov (United States)

... Loss of Heterozygosity in Normal Breast Epithelial Tissue and Benign Breast Lesions in BRCA1/2 Carriers with Breast Cancer. ...

2004-09-01

185

Accumulation and distribution of "8"5Kr in rats exposed to "8"5Kr atmospheres  

International Nuclear Information System (INIS)

Retention kinetics and tissue partition coefficients for "8"5Kr were determined in rats exposed to known concentrations of "8"5Kr gas. The tissue partition coefficients were used to calculate the relative tissue radiation dose, assuming exposure conditions equivalent to the maximum permissible concentration in air (MPC)a. The estimated radiation dose was highest in adrenals and body fat, about twice the dose estimated for the next highest tissue, ovaries. Saturation and desaturation curves show that the contents of the large intestines have a prolonged desaturation time compared to other samples analyzed.

1977-05-01

186

Recrystallization in AZ31 magnesium alloy during hot deformation  

Energy Technology Data Exchange (ETDEWEB)

In this study, isothermal torsion tests were carried out on magnesium AZ31B alloy under constant strain rate conditions, in the range of 250 to 400 C at 0.01, 0.1, and 1.0 s{sup -1}. Alloy flow stress dependence on strain rate and temperature can be described by a power law with activation energy of 130 kJ/mol. Microstructural examination of hot deformed samples shows very fine recrystallized grains decorating grain boundaries of larger gains in the form of a necklace. These fine grains are produced by dynamic recrystallization at the grain boundaries of original grains. Microstructure evolution, based on samples quenched at different strain levels, indicates that increasing deformation strain has little effect on recrystallized grain size but widens the recrystallized region, with full recrystallization achieved at a certain high strain level. Recrystallized grain size increases ...

2005-07-01

187

Nb{sub 3}Sn LLNL cable test strand  

Energy Technology Data Exchange (ETDEWEB)

Axial strain characterization was completed for a Nb{sub 3}Sn conductor from Lawrence Livermore National Lab used in a transverse stress test of a cable-in-conduit conductor. The effect of axial strain on the critical current of the LLNL Nb{sub 3}Sn test strand was measured at magnetic fields from 8 T to 22 T. The conductor specifications are shown in Table 3, and the measured data are shown in Table 4. The data are presented graphically in Figs. 5 through 7. The I{sub c} and J{sub c} values are based on an electric field criterion (E{sub c}) of 2 {mu}V/cm. The zero-strain 12 T value of J{sub c} at 12 T was 0.39 GA/m{sup 2}. The irreversible strain limit was quite high, 1.0 strain, and the compressive prestrain was relatively low at 0.27%. Fracture strain was 1.10%.

1994-01-01

188

Microscopic investigations of microcrack formation in two ferritic-martensitic steels in nonirradiated and irradiated conditions; Mikroskopische Untersuchung der Bildung von Ermuedungsrissen an zwei ferritisch-martensitischen Staehlen im unbestrahlten und vorbestrahlten Zustand  

Energy Technology Data Exchange (ETDEWEB)

The low-cycle fatigue properties of the ferritic-martensitic steels MANET I and II and F82H-mod, have been investigated at a temperature of 200 C. The strain-controlled fatigue has been performed with strain ranges between 0,4% and 1,0% and at a strain rate of 8.10{sup -4} s{sup -1}. The steel F82H-mod, is characterised by a higher plastic strain amplitude compared to MANET I and II. Some of the specimens have beenirradiated with a 104 MeV {alpha}-particle beam. The homogeneous implantation of 400 appm He leads to a hardening of the material. At the beginning of the fatigue tests the stress response of the irradiatedspecimens is higher compared to the unirradiated specimens. In general, thematerial shows softening during fatigue. The softening of irradiated material, which is fatigue tested with a high total strain amplitude, is higher than that of the unirradiated material. The ...

1997-09-01

189

Tissue culture process for the clonal production of loblolly pine plantlets  

Energy Technology Data Exchange (ETDEWEB)

A tissue culture process, organogenesis, is described which involves the stepwise production of a shoot apex, which grows to have leaves and stem and finally roots, culminating in the production of a complete plant. The production and subsequent development of numerous adventitious shoots, preferably from adult tree tissues offers the greatest potential for commercial cloned plantlet production. The process describes these steps for Pinus taeda, the loblolly pine. (Refs. 25).

1981-02-01

190

Determination of americium and plutonium in autopsy tissue: methods and problems  

International Nuclear Information System (INIS)

The current methods used by the tissue analysis program at LASL for the determination of americium and plutonium in autopsy tissue are described. Problems affecting radiochemical yield are discussed. Included are problems associated with sample preparation, separation of plutonium from large amounts of bone ash, and reagent contamination. The average "2"4"2Pu tracer yield for 1800 Pu determinations is 78 +- 12%. The average "2"4"2Am tracer yield is 85 +- 7% for 40 determinations.

191

Determination of americium and plutonium in autopsy tissue: methods and problems  

Science.gov (United States)

The current methods used by the tissue analysis program at LASL for the determination of americium and plutonium in autopsy tissue are described. Problems affecting radiochemical yield are discussed. Included are problems associated with sample preparation, separation of plutonium from large amounts of bone ash, and reagent contamination. The average /sup 242/Pu tracer yield for 1800 Pu determinations is 78 +- 12%. The average /sup 242/Am tracer yield is 85 +- 7% for 40 determinations.

1979-01-01

192

Contribution to the in vitro multielement X-ray fluorescence analysis of human soft tissues  

International Nuclear Information System (INIS)

Determination of Mn, Fe, Ni, Cu, Zn, and Pb in human soft tissues by radionuclide X-ray fluorescence analysis (using "2"3"8Pu and "1"0"9Cd sources) is described. The results of multielement XRS analysis of selected human tissues are tabulated. They are in good agreement with the literature data. It is concluded that X-ray fluorescence technique is a suitable method for trace element determination. (author).

1986-11-01

193

Barrier filter for fluorescence microscopy of strongly autofluorescent plant tissues. Application to actin cables in Chara.  

Science.gov (United States)

A liquid barrier filter for use in fluorescence microscopy of strongly autofluorescent plant tissues is described. The filter consists of a methanol solution of cupric chloride and ferric chloride and isolates fluorescein fluorescence from the strong red autofluorescence of photosynthetic plant tissues. Subcortical actin cables in the giant alga Chara are being visualized through use of this filter together with heavy meromyosin labeling. PMID:90068

1979-05-01

194

Use of the K88 antigen for in vivo bacterial competition with porcine strains of enteropathogenic Escherichia coli.  

Science.gov (United States)

Infant mice were used to measure the amount of fluid accumulation (enterosorption) in the intestinal tract after oral inoculation of a porcine strain of enteropathogenic Escherichia coli (K88-+, Ent-+). Significant reduction in the amount of fluid found in the intestinal tract was observed if the mice were first inoculated with a K88-possessing, non-enterotoxigenic strain of E. coli. The protection provided is thought to be due to specific competition for attachment sites on cells of the small intestine. PMID:1095492

1975-07-01

195

The viscoelastic composite with interface debonding  

British Library Electronic Table of Contents (United Kingdom)

The effect of particle/matrix interface debonding on composites consisting of elastic particles and viscoelastic matrix is studied. The interface debonding is represented by a nonlinear cohesive law for the high explosive PBX 9501. The Mori-Tanaka method gives the constitutive relation of the composite in terms of the properties of elastic particles, viscoelastic matrix, and nonlinear cohesive law for interfaces. For the example of a composite with spherical particles subject to hydrostatic tension, simple analytical expressions of the composite stress-strain relation are obtained. The strain rate and temperature have strong effects on the composite behavior. High strain rate and low temperature give high strength of the composite. However, the rate and temperature effects decrease as the ...

2008-01-01

196

Superplastic behaviour in Ti-3Al-4V  

International Nuclear Information System (INIS)

Superplastic deformation behavior in an alpha + beta titanium alloy Ti-3A-4V has been investigated by using the method of cross head speed cycling in the temperature range 750-910 /sup 0/C. On the basis of flow stress-strain rate-strain rate sensitivity data, the alloy was found to be superplastic in the entire temperature range studied. Optimum superplasticity was observed in the strain-rate range 3 x 10/sup -5/ sec./sup -1/ to 6 x 10/sup -5/ and at the highest temperature of 910 deg. C. (author).

197

Probabilistic fracture assessment of surface cracked pipes using strain-based approach  

British Library Electronic Table of Contents (United Kingdom)

Simplified strain-based fracture mechanics equations, established for external surface cracked pipelines subjected to an external bending load, are presented and used in probabilistic assessment of a pipeline girth weld. The model takes into account several parameters, such as variation in crack depth, crack length, internal pressure and material hardening. The critical strain from ductile tearing in the cracked pipeline is found by using the tangency criterion. The reliability problem is solved using first and second order reliability methods for different pipe dimensions and load levels.

2006-01-01

198

Phenomenological modeling of the stress-strain behaivor of ceramic matrix composites with application to stress analysis  

Energy Technology Data Exchange (ETDEWEB)

A plane stress constitutive relation is developed for laminate composites undergoing matrix cracking and therefore displaying significant nonlinear stress-strain behavior. The constitutive relation is similar in some respects to a phenomenological theory of plasticity in that it is fit to data from uniaxial tests. The constitutive equations are specialized to brittle matrix composites (especially CMCs) in the form of cross-piles and quasi-isotropic laminates. Several problems involving stress redistribution at holes and notches due to nonlinear stress-strain behavior are investigated with the aid of the constitutive law.

1995-12-31

199

Pathogenic and nonpathogenic Acanthamoeba spp. in thermally polluted discharges and surface waters  

International Nuclear Information System (INIS)

During spring and autumn, the total number of amoebae and the number of acanthamoeba species able to grow at 37 degrees C were determined in six thermally polluted factory discharges and the surrounding surface waters. The isolated Acanthamoeba strains were studied for growth in axenic medium, cytopathic effect in Vito cell cultures, and virulence in mice. Although more amoebae were isolated in autumn, the number of Acanthamoeba species was lower than in spring, when the percent of pathogenic strains among the isolates was highest. Higher concentrations of amoebae were found in warm discharges, and more virulent strains occurred in thermal discharges than in surface waters.

200

Magnetic Properties of a Material Used to Estimate Elastic and Plastic Strains of Ferrite?Pearlite Steels  

British Library Electronic Table of Contents (United Kingdom)

The effect of elastic and plastic strains on the magnetic properties of ferrite-pearlite steels has been studied. It has been shown that the sensitivity to elastic-tensile and bending strains is four to five times greater for remnant magnetization than for coercive force. In order to determine the degree of cold plastic deformation of high-carbon steels, a two-parametric testing technique based on the use of remnant magnetization and coercive force was suggested. An MMT-2 device was recommended for measurement of the required parameters.

2005-01-01

201

Experimental and analytical studies of four-inch pipe whip tests under PWR LOCA conditions  

Energy Technology Data Exchange (ETDEWEB)

In the tests, the effects of the overhang length on the pipe whip behavior of the pipe-restraints system were studied by measuring the strains and deformations of the test pipe and restraints, and the restraints forces. The equation for predicting the maximum strain at the outer surface of the pipe was derived using a static equilibrium condition. The calculated maximum strains at the outer surface of the pipe agree fairly well with experimental data. The dynamic response analysis of the pipe-restraints system was conducted by the finite element program ADINA. The applicability of the ADINA program to the pipe whip analysis is made clear through this analysis.

1984-01-01

202

Effects of FIB milling and pre-straining on the microstructure of directionally solidified Mo pillars: a Laue diffraction analysis  

Energy Technology Data Exchange (ETDEWEB)

White beam Laue micro-diffraction was performed on directionally solidified, single-crystal Mo pillars in the as-grown state, after focused ion beam (FIB) milling and after pre-straining. The Laue diffraction peaks from the as-grown pillars are very sharp and show no broadening, similar to those from single-crystal Si wafers. Significant broadening and streaking of the peaks occurred after FIB milling and pre-straining, indicative of the damage these treatments induce in the nearly perfect crystal structure of the directionally solidified Mo pillars.

2010-05-01

203

Changes in the structure and superconducting transition temperature of A-15 compounds under the effect of high pressure and shear strain  

Energy Technology Data Exchange (ETDEWEB)

The study is concerned with the combined effect of high pressure and shear strain on the properties of the A-15 intermetallics Nb3Sn and Nb3Al0.75Ge0.25. The properties discussed are the superconducting transition temperature, the width and intensity of the X-ray diffraction lines, and the width and shape of the Mossbauer absorption lines. It is shown that the combined effect of high pressure and shear strain leads to an intense generation of defects in polycrystalline specimens; the size of the ordered regions does not exceed 300 A even under the least severe conditions. 5 references.

1985-01-01

204

A micro-compression study of shape-memory deformation in U-13at%Nb  

Energy Technology Data Exchange (ETDEWEB)

Micro-compression specimens, 1O-15{mu}m in diameter by 20-30{mu}m in height, were produced from individual parent grains in a polycrystalline U-13at%Nb shape-memory alloy using the focused ion beam (FIB) technique. The specimens were tested in a nanoindentation instrument with a flat diamond tip to investigate stress-strain behavior as a function of crystallographic orientation. The results are in qualitative agreement with a single-crystal accommodation strain (Bain strain) model of the shape-memory effect for this alloy.

2008-01-01

205

UV inactivation: Combined effects of UV radiation and xenobiotics in two strains of Saccharomyces  

International Nuclear Information System (INIS)

The effects of eight chemicals on the inactivation rate of ultraviolet radiation on the colony building capabilities of two strains of Saccharomyces cervisae - a wild type strain and a mutant deficient in excision repair - were studied. The insecticide methoxychlor, the herbicide 2,4-dichlorophenoxyacetic acid, the fungicide pentachlorophenol and its metabolite tetrachlorohydroquinone, as well as the chemicals acrylonitrile and 2,3-dichloro-1-propene have no significant impact on the effects of UV radiation in Saccharomyces cerevisae. Depending on the concentration, trichloroethylene increases the sensitivity to UV radiation. The herbicide paraquat provides efficient protection against UV radiation at concentrations where a toxic effect cannot be observed even without UV. The results were rather similar for both strains. (orig.).

206

Tensile strain limits of buried defects in pipeline girth welds  

Energy Technology Data Exchange (ETDEWEB)

There are currently no accepted industry standards for the tensile strain limits of girth welds. This paper investigated the behavior of girth welds with buried defects subjected to high longitudinal strains caused by soil movement. A strain design methodology based on a crack driving method was used to examine the factors influencing stain limits along with a constraint-sensitive fracture mechanics approach. No strength undermatching was used in the welds, and the defect location had no influence on crack driving force. The weld joint was assumed to have uniform tensile properties. A 3D finite element (FE) model was used to simulate pipe behavior. Symmetric boundary conditions were imposed on the symmetry planes, and uniform remote axial displacement was applied as the primary loading. Automated data processing routines were developed to extract and analyze the data. The crack driving force was computed directly from a ...

2004-07-01

207

Studies on insecticide-resistant anophelines  

UK PubMed Central (United Kingdom)

The development of several DDT-resistant strains of Anopheles atroparvus is described. They were selected by larval treatment only (RL), selection of larval and adult females (RLAF),...Full Text Available

1959-01-01

208

Structural integrity of whipping pipes following postulated rupture - a contribution to strain levels acceptable under faulted conditions. Integritaetsverhalten von Rohrleitungen nach unterstelltem Rundabriss - ein Beitrag zur Dehnungsabsicherung bei Schadensfaellen  

Energy Technology Data Exchange (ETDEWEB)

By tests about pipe failure under extreme bending, moment at cross sections affected by circumferential cracks, admissible strains for securing the integrity in the area of a flow link are indicated. These are 7% for austenic and ferritic materials in the undisturbed cross section. From d/t-ratios of 20 onwards the strains are limited by pipe bulging on the pressure-load side. In this connection, circumferential flaws do not have any influence on the behaviour on the strained side, when their expansion does not exceed the investigated limitis of <60 circumferential expansion and crack depth of 0,3 t. (orig.)

1991-01-01

210

Strain gauge validation experiments for the Sandia 34-meter VAWT (Vertical Axis Wind Turbine) test bed  

Science.gov (United States)

Sandia National Laboratories has erected a research oriented, 34- meter diameter, Darrieus vertical axis wind turbine near Bushland, Texas. This machine, designated the Sandia 34-m VAWT Test Bed, is equipped with a large array of strain gauges that have been placed at critical positions about the blades. This manuscript details a series of four-point bend experiments that were conducted to validate the output of the blade strain gauge circuits. The output of a particular gauge circuit is validated by comparing its output to equivalent gauge circuits (in this stress state) and to theoretical predictions. With only a few exceptions, the difference between measured and predicted strain values for a gauge circuit was found to be of the order of the estimated repeatability for the measurement system.

1988-08-01

211

Strain differences in the responsiveness between Sprague-Dawley and Fischer rats to nephropathy induced by FYX-051, a xanthine oxidoreductase inhibitor  

British Library Electronic Table of Contents (United Kingdom)

To determine a rat strain appropriate for carcinogenicity testing of FYX-051, a xanthine oxidoreductase inhibitor, we performed a 4-week oral toxicity study by administering 0.3, 1 and 3?mg/kg, and 1, 3 and 10?mg/kg of FYX-051 to male Sprague-Dawley (SD) and Fischer (F344) rats, respectively. Histopathology revealed that the degree of FYX-051-induced nephropathy was 3-fold stronger in SD rats than in F344 rats. Our previous study demonstrated that the key factor of species differences in FYX-051-induced nephropathy is purine metabolism. This observation led us to examine the involvement of purine metabolism in differences among two strains of rats. However, purine metabolism was proven not to be implicated as an important factor. Subsequently, other factors responsible for the strain diffe...

2006-01-01

212

Strain Rate Effects in SA-106 Carbon Steel Pipe,  

Science.gov (United States)

... rate on the tensile properties of SA-106 carbon steel pipe, in support of analysis and experimental modeling of postulated pipe whip in nuclear ...

1982-02-01

213

SiGeC materials  

Energy Technology Data Exchange (ETDEWEB)

The growth and properties of Si{sub 1{minus}y}C{sub y} and Si{sub 1{minus}x{minus}y}Ge{sub x}C{sub y} alloys pseudomorphically strained on Si(001) will be reviewed. Although the bulk solubility of carbon in silicon is small, epitaxial layers with more than 1 at.% C can be fabricated. The relation between substitutional and interstitial carbon incorporation will be presented. Substitutionally incorporated C atoms allow strain manipulation, including the growth of strain-free or inversely strained Si{sub 1{minus}x{minus}y}Ge{sub x}C{sub y} layers. The mechanical properties, microscopic structure, thermal stability, as well as the influence of C atoms on band structure will be discussed.

1996-12-31

214

Production of Amylase in Liquid Culture by a Strain of Aspergillus oryzae  

UK PubMed Central (United Kingdom)

The effect of different media and pH on the formation of amylase by Aspergillus oryzae EI 212 is described. Depending upon the composition of the medium and growth...Full Text Available

1970-04-01

215

Plasmid-Determined Copper Resistance in Pseudomonas syringae from Impatiens  

UK PubMed Central (United Kingdom)

A strain of Pseudomonas syringae was recently identified as the cause of a new foliar blight of impatiens. The bacterium was resistant to copper compounds, which are used on a variety...Full Text Available

1990-01-01

216

Phylogenetic Characterization of Virulence and Resistance Phenotypes of Pseudomonas syringae  

UK PubMed Central (United Kingdom)

Individual strains of the plant pathogenic bacterium Pseudomonas syringae vary in their ability to produce toxins, nucleate ice, and resist antimicrobial compounds. These phenotypes...Full Text Available

2005-09-01

217

Non-toxigenic Clostridium sordellii: Clinical and microbiological features of a case of cholangitis-associated bacteremia  

British Library Electronic Table of Contents (United Kingdom)

Toxigenic Clostridium sordellii strains are increasingly recognized to cause highly lethal infections in humans that are typified by a toxic shock syndrome (TSS). Two glucosylating toxins, lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are believed to be important in the pathogenesis of TSS. While non-toxigenic strains of C. sordellii demonstrate reduced cytotoxicity in vitro and lower virulence in animal models of infection, there are few data regarding their behavior in humans. Here we report a non-TSS C. sordellii infection in the context of a polymicrobial bacterial cholangitis. The C. sordellii strain associated with this infection did not carry either the TcsL-encoding tcsL gene or the tcsH gene for TcsH. In addition, the strain was neither cytotoxic in vitro nor lethal in a murine...

2011-01-01

219

Inactivation of several strains of Listeria monocytogenes attached to the surface of packaging material by Na-Chlorophyllin-based photosensitization  

British Library Electronic Table of Contents (United Kingdom)

This study was focused on the possibility to inactivate thermosensitive Listeria monocytogenes ATCL3C 7644 and thermoresistant 56 Ly strain by Na-Chlorophyllin (Na-Chl)-based photosensitization in vitro and on the surface of packaging. Comparative analysis of antimicrobial efficiency of photosensitization with conventional surface cleaning was performed. Data indicate that both Listeria strains, after incubation with Na-Chl and following illumination (l=400nm, 20mWcm-2), were inactivated by 7 log in vitro. This treatment cleaned both Listeria strains from packaging surfaces. Comparative analysis indicates that washing with water diminishes pathogens by less than 1 log, 200ppm Na-hypochlorite by 1.7 log, Na-Chl-based photosensitization by 4.5 log. Listeria biofilms were totally removed from...

2010-01-01

220

In vitro susceptibility of enterococci strains isolated from urine samples to fosfomycin and other antibiotics  

British Library Electronic Table of Contents (United Kingdom)

Enterococci are mostly isolated from urinary tract infections (UTIs). Increasing antibiotic resistance affects the success rate in empirical treatment, thus makes antibiotic susceptibility tests important. The aim of this study was to determine the species distribution and resistance patterns of Enterococcus strains isolated from urine samples to antibacterial agents including fosfomycin in a teaching hospital, Istanbul. The identification of 100 Enterococcus strains were determined by using conventional methods and API 20 Strep (bioMerieux France). The susceptibility testing was performed by disk diffusion and Etest. The majority of isolates were E. faecalis (67%), followed by E. faecium (33%). The ratio of E. faecalis to E. faecium was 2.03/1. E. faecalis and E. faecium strains were resi...

2011-01-01

221

High strain rate properties of composites  

Science.gov (United States)

Polymer matrix composites are presently used in many fields due to their excellent mechanical properties such as high specific strength and stiffness. However, not much is known of these composites' response to impact loading, which usually occurs at strain rates much higher than the strain rates used to measure the quasi-static mechanical properties of materials. The use of static properties in the study of structures that undergo dynamic loading can produce excessive design weight or cause unexplained and untimely failure. The studies included in this dissertation provide the results of Split Hopkinson Pressure Bar experiments to determine the ultimate compressive strength and strain of composites at high strain rates. Most researchers are using specimens with different dimensions and geometry. For this reason, it is difficult to compare results of tests done by different people. Herein, the Split ...

1998-01-01

222

GENETIC STUDIES OF PIGMENTATION IN ...  

Science.gov (United States)

... Strains Smith and -aybush were both grown in broth containing from 0.1 to 50 pg/ml of acriflavin, acridine orange, or proflavin. ...

1966-05-01

223

Free-living amoebae as vectors of cryptosporidia  

British Library Electronic Table of Contents (United Kingdom)

In the present article, the study to examine the ability of free-living amoebae (FLA) to serve as vectors of cryptosporidia is presented. Ten strains of different free-living amoebae of the FLA collection of the Parasitology Lab at Koblenz were cultivated in the presence of Cryptosporidium parvum oocysts. After phagocytosis and ingestion, the oocysts could be found in food vacuoles within the cytoplasm of the trophozoites of two different FLA strains. The uptake and the transport of the oocysts within the trophozoites could be demonstrated in an Acanthamoeba sp. (group II) strain (maximum, three oocysts; average, one oocyst) as well as in a Thecamoeba quadrilineata strain (maximum, 15 oocysts; average, eight oocysts), with the help of light microscopy. We found that these free-living amoeb...

2011-01-01

225

Experimental Immunization with Live Strains of Bacterium ...  

Science.gov (United States)

... The serum shows an agltnation titer of between 80 and 320 aainst Bsaterium tulganse and 0-40 a ainst Brucela Bang (*ompathetic agglutination ...

1968-07-01

226

Excitonic transitions in InGaP/InAlGaP strained quantum wells  

Science.gov (United States)

Excitonic transitions in metalorganic vapor phase epitaxially grown In[sub [ital x

1993-08-30

227

Effect of welding parameters on hot cracking susceptibility of alloy 800  

Energy Technology Data Exchange (ETDEWEB)

The varestraint cracking test was carried out to assess the hot cracking tendency of alloy 800 at different welding conditions and augmented strain levels. The test results revealed that the hot cracking susceptibility of alloy 800 increases with increasing the heat input and augmented strain levels. This emphasizes the role of constraint either internally induced or externally applied to the welded structure. A cracking threshold or the minimum augmented strain ([epsilon][sub min]) below which no hot cracking takes place has been found to be 0.25 for alloy 800. Also, the Critical Strain Rate to Time (CSS) required to cause cracking was found to be 6.42x10[sup -3] sec[sup -1] at welding condition of 100 Amp, 13 Volt and 140 mm/min. (author).

1994-08-01

228

Effect of welding parameters on hot cracking susceptibility of alloy 800  

International Nuclear Information System (INIS)

The varestraint cracking test was carried out to assess the hot cracking tendency of alloy 800 at different welding conditions and augmented strain levels. The test results revealed that the hot cracking susceptibility of alloy 800 increases with increasing the heat input and augmented strain levels. This emphasizes the role of constraint either internally induced or externally applied to the welded structure. A cracking threshold or the minimum augmented strain (#epsilon#_m_i_n) below which no hot cracking takes place has been found to be 0.25 for alloy 800. Also, the Critical Strain Rate to Time (CSS) required to cause cracking was found to be 6.42x10"-"3 sec"-"1 at welding condition of 100 Amp, 13 Volt and 140 mm/min. (author).

229

Effect of salt stress and phosphorus deficiency in mutants of rhizobium obtained by gamma irradiation  

International Nuclear Information System (INIS)

Two strains of Rhizobium: Rhizobium Tropici and Mesorhizobium Ciceri nodulating respectively common bean and chickpea were treated by gamma irradiation (60Co) source. Radiosensibility analysis showed that 800 Gy was the biggest dose supported by these two strains. We isolated gamma irradiated resistant strain in order to select mutant of them which can supported salt stress and phosphorus deficiency. Salinity analysis showed that Mesorhizobium Ciceri 835 strain, can tolerate up to 18g/l (273 mM NaCl) of salt, whereas, their irradiation mutants tolerate salinity up to 33g/l (564mM. NaCl) Rhizobium Tropici CIAT899 can survive at 20g/l (342 mM) either for control strain or mutants. Analysis of phosphorus deficiency showed that either Rhizobium Tropici CIAT899, or Mesorhizobium Ciceri 835 can survive in medium without phosphore. Our results permit us to screen mutants tolerant to these ...

2008-08-12

230

Effect of microstructure and deformation conditions on hot plasticity of Ti-6Al-5Mo-5V-1Cr-1Fe titanium alloy  

International Nuclear Information System (INIS)

Results of investigation on the effect of microstructure and deformation conditions (temperature and strain rate) on flow stress and critical strain in two phase #alpha# + #beta# titanium alloy Ti-6Al-5Mo-5V-1Cr-1Fe is presented in the paper. The alloy is deformed or torsional plastometer at temperature range 1073-1173 K and at the strain rate #epsilon# = 0.04-4.0 s"-"1. Sequential deformation at 1173 K at the strain rate of 0.4 s"-"1 was also performed with holding times in the range 1-100 s. The fraction of dynamically and metadynamically recrystallized #beta# phase was determined on the basis of results of sequential deformation test. Microstructure was examined using LM and TEM techniques. (author)

2004-06-20

231

Effect of Stress and Strain Path on Cavity Closure During Hot ...  

Science.gov (United States)

... CLOSURE DURING HOT WORKING OF AN ALPHA/BETA TITANIUM ALLOY (PREPRINT) ... HOT WORKING OF AN ALPHA/BETA TITANIUM ALLOY ...

2007-07-01

232

Development of a Suite of Luciferase Gene Probes for the ...  

Science.gov (United States)

... between 2 strains is acquired by adding the lengths of the connecting branches, using the scale which depicts 1 amino acid substitution per 10 ...

2011-05-15

233

Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria  

British Library Electronic Table of Contents (United Kingdom)

Studies were carried out on the decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Among the 27 strains of halophilic and halotolerant bacteria isolated from effluents of textile industries, three showed remarkable ability in decolorizing the widely utilized azo dyes. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that these strains belonged to the genus Halomonas. The three strains were able to decolorize azo dyes in a wide range of NaCl concentration (up to 20%w/v), temperature (25-40degreeC), and pH (5-11) after 4 days of incubation in static culture. They could decolorize the mixture of dyes as well as pure dyes. These strains also readily grew in and decolorized the high concentrations of dy...

2007-01-01

234

DYNAMIC TESTS OF MODEL CONCRETE.  

Science.gov (United States)

... 4 maximum aggregate size. The effects of strain rate (up to 2.5 in./in./sec) on ultimate compressive strength were obtained. ...

1969-11-01

235

DNA methods for identification of Chinese medicinal materials  

UK PubMed Central (United Kingdom)

As adulterated and substituted Chinese medicinal materials are common in the market, therapeutic effectiveness of such materials cannot be guaranteed. Identification at species-, strain- and locality-levels,...Full Text Available

236

Complete genome sequences of Brucella melitensis strains M28 and M5-90, with different virulence backgrounds.  

Science.gov (United States)

Brucella melitensis is a Gram-negative coccobacillus bacteria belonging to the Alphaproteobacteria subclass. It is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. The B. melitensis strain M5-90, a live attenuated vaccine cultured from the B. melitensis virulent strain M28, has been an effective tool to control brucellosis in goats and sheep in China. Here we report the complete genome sequences of B. melitensis M28 and M5-90, strains with different virulence backgrounds, which will serve as a valuable reference for future studies. PMID:21478357

2011-04-08

237

Comparison of the Influence of Temperature on the High-Strain-Rate Mechanical Responses of PBX 9501 and EDC37  

British Library Electronic Table of Contents (United Kingdom)

Many high-strain-rate compression measurements (2000 per second) using a specially designed split Hopkinson pressure bar (SHPB) for the plastic-bonded explosive PBX9501 have been reported in the literature, but there is a sparsity of data for a United Kingdom polymer-bonded explosives (PBX) known as EDC37. Both EDC37 and PBX9501 are cyclotetramethylenetetranitramine-based (HMX-based) PBXs with high filler contents. The binder systems for the PBXs are very different: EDC37 consists of a nitroplasticized nitrocellulose and PBX9501 a nitroplasticized ESTANE. PBX9501 exhibits nearly invariant fracture strains of ?1.5?pct as a function of temperature at high strain rates, whereas EDC37 fails at ?2 to 2.5?pct. The maximum compressive strengths for both PBXs were measured at 150?Mpa at ?55??C, bu...

2008-01-01

238

Characterization of cry Genes in a Mexican Bacillus thuringiensis Strain Collection  

UK PubMed Central (United Kingdom)

Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis...Full Text Available

1998-12-01

239

Capsular Serotype and Antibiotic Resistance of Streptococcus pneumoniae Isolates in Two Chilean Cities  

UK PubMed Central (United Kingdom)

We compared the incidence of nasopharyngeal colonization by Streptococcus pneumoniae, the serotypes causing mucosal and invasive diseases, and the antibiotic resistance of these strains...Full Text Available

1998-03-01

240

Bioterrorism and Biocrimes: The Illicit Use of Biological Agents ...  

Science.gov (United States)

... view on the utility of such attacks, see JH Rothschild, Tomorrow's Weapons ... Aum Shinrikyo may have had a stolen vaccine anthrax strain incapable ...

2001-02-01

241

Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance  

British Library Electronic Table of Contents (United Kingdom)

In this study, bacterial strains were investigated in order to determine their heavy metal tolerance. The bacterial strains were identified as Bacillus cereus and Bacillus pumilus. In the batch system, the effects of operating variables such as solution pH, initial metal concentration, contact time, and adsorbent dosage were investigated. Both isolates were highly resistance to copper and lead in comparison with the control strain examined. The adsorption capacities of B. cereus and B. pumilus were found to be 22.1mg/g and 28.06mg/g, respectively. The biosorption follows pseudo-second order kinetics and the isotherm fits well to the Langmuir isotherm model. In column experiments, the biosorption was fitted well by the Thomas model. The breakthrough and exhaustion capacity of each biosorben...

2011-01-01

243

Antimicrobial Susceptibility of Haemophilus ducreyi  

UK PubMed Central (United Kingdom)

The susceptibility of 19 isolates of Haemophilus ducreyi from a recent chancroid outbreak and four reference strains was determined in vitro to 13 antimicrobial agents. The rabbit intradermal...Full Text Available

1978-04-01

244

Antimicrobial Ointments and Methicillin-Resistant Staphylococcus...  

Science.gov (United States)

Staphylococcus aureus isolates and 2 USA300 ATCC type strains for susceptibility to bacitracin and neomycin contained in over-the-counter antibacterial ointments. Resistance to...

2011-10-15

245

Analytical Summary. Part 4. The Theory of Armor Penetration  

Science.gov (United States)

... F ? (28.3)(108) in an isotherme.l deformation with stationary principal axes of strain rate, but the component might be as small as F2= (19-3)(100) ...

1946-05-01

246

Alcohol fermentation in olive oil extraction effluents  

Energy Technology Data Exchange (ETDEWEB)

Eight culture-collection yeast strains of various species and five newly isolated stains were tested for both growth in olive oil extraction effluents and fermentation of the sugars in the same media. The culture-collection yeast strains did not grow in an effluent containing 2.86% sugar (w/v), 8 g/litre phenolic substances, 4.58 g/litre titratable acidity and pH 4.96, whereas the newly isolated strains of Torulopsis sp. MK-1, Saccharomyces norbensis MC-1, S. oleaceus MC-2 and S. oleaginosus grew well and fermented the sugars. In the medium mentioned above, they produced alcohol in amounts of 1.63 to 1.38%, respectively. None of the yeasts grew in an olive oil extraction effluent vacuum-concentrated to over 13-14% of dry matter. The strain of T. sp. MK-1 showed a higher stability.

1989-01-01

247

Activation of the alternative pathway of complement by mycobacteria and cord factor.  

UK PubMed Central (United Kingdom)

The ability of a number of mycobacteria and some of their components to activate complement was examined. Mycobacterium bovis BCG (Glaxo strain), Mycobacterium leprae, Mycobacterium lepraemurium, and...Full Text Available

1980-07-01

248

Activation energy for high temperature deformation of Ti-10V alloy on the basis of second strain rate cycles  

International Nuclear Information System (INIS)

Activation energy values for high temperature deformation of a beta -titanium alloy Ti-10V were determined from the flow stress strain rate data taken from the second increasing strain rate cycles, in order to find the rate controlling mechanism. Although the data obtained from first increasing strain rate cycles are considered most appropriated by the author in a previous study due to obvious reasons, the values obtained in present work are again indicative of lattice self diffusion in beta -phase as the rate controlling mechanism for this alloy and nevertheless suggest that two different activation energy values for a deformation process could be possible in a given temperature range, depending upon the data. (author)

2007-12-01

249

Procedure for radiation dose control in irradiated tissues during electron-beam therapy  

International Nuclear Information System (INIS)

The invention refers a procedure of radiation dose control in irradiated tissues during electron-beam therapy. It aims at meeting the planned radiation dose for diseased tissues and taking care of the healthy ones. Therefore, the dose distribution required is determined before irradiation in consideration of such factors as energy-dependence of detector sensitivity, self-absorption within the tissue, and relative biological effectiveness. Furthermore, the expected intensity distribution of secondary quantum radiation excited in the irradiated tissue is calculated. A radiation detector for local resolution is used for registration. During irradiation the calculated intensity distribution is compared with the measured one. The invention is applicable in radiation therapy with monoenergetic electron beams.

1984-11-08

250

Accumulation of glycosaminoglycans in radiation-induced muscular fibrosis  

Energy Technology Data Exchange (ETDEWEB)

The content and biosynthesis of glycosaminoglycans (GAGs) were studied in pig thigh muscle after acute local {gamma}-irradiation. Seven months following irradiation, the muscular tissue next to the irradiation cone was replaced by severe mutilating fibrosis delimited by an intermediary perifibrotic zone. Results showed a parallel increase of collagen and GAG content in perifibrotic and fibrotic tissues. Sulphated GAGs, heparan sulphate and dermatan sulphate were preferentially accumulated in fibrotic tissue, while the hyaluronic acid content increased only slightly. Synthesis of sulphated GAGs was more elevated in fibrotic tissue than in perifibrotic zone as compared with normal muscle. Seven months after irradiation well-developed fibrotic tissue continued to synthesize and to accumulate extracellular matrix macromolecules. (Author).

1992-05-01

251

Transplantation of engineered bone tissue using a rotary three-dimensional culture system.  

Science.gov (United States)

Bone is a complex, highly structured, mechanically active, three-dimensional (3-D) tissue composed of cellular and matrix elements. We previously published a report on in situ collagen gelation using a rotary 3-D culture system (CG-RC system) for the construction of large tissue specimens. The objective of the current study was to evaluate the feasibility of bone tissue engineering using our CG-RC system. Osteoblasts from the calvaria of newborn Wistar rats were cultured in the CG-RC system for up to 3 wk. The engineered 3-D tissues were implanted into the backs of nude mice and calvarial round bone defects in Wistar rats. Cell metabolic activity, mineralization, and bone-related proteins were measured in vitro in the engineered 3-D tissues. Also, the in vivo histological features of the transplanted, engineered 3-D tissues were evaluated in the animal models. ...

2007-03-11

252

[Computed tomography in gunshot trauma. I. Ballistics elements and the mechanisms of the lesions].  

Science.gov (United States)

The knowledge of wound ballistics and of wounding mechanisms is mandatory for the radiologist to interpret the CT findings of gunshot wounds. The severity of a bullet wound depends on the characteristics of the tissue it goes through, i.e., tissue elasticity, density, thickness of the wounded body part, the type of tissue, its specific gravity, internal cohesiveness and anatomical relationships, as well as on missile characteristics, i.e., mass, shape, fragmentation and deformation. Bullet velocity is certainly a major factor in wounding, but it is only one factor. Two major wounding mechanisms exist the crushing of the bullet-struck tissue (forming the permanent cavity) and radial stretching (forming a temporary cavity). Bullet "yaw" is defined as the angle between the long axis of the bullet and its flight path. The yaw is directly proportional to tissue crushing and stretching: ...

1995-04-01

253

Thio Wax Ester Biosynthesis Utilizing the Unspecific Bifunctional Wax Ester Synthase/Acyl Coenzyme A:Diacylglycerol Acyltransferase of Acinetobacter sp. Strain ADP1  

UK PubMed Central (United Kingdom)

The bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT) from Acinetobacter sp. strain ADP1 (formerly Acinetobacter calcoaceticus...Full Text Available

2005-02-01

254

Studies on a Factor in Sweet Potato Root Which Agglutinates Spores of Ceratocystis fimbriata, Black Rot Fungus 1  

Science.gov (United States)

A factor which agglutinated the spores of Ceratocystis fimbriata in the presence of Ca2+ was purified from sweet potato (Ipomea batatas Lam cv. Norin[1]) root. Element composition of the purified factor was as follows; analysis found: C (29.8%), H (3.97%), O (65.34%), N (0.81%): calculated for C43H69O70N1: C (30.02%), H (4.01%), O (65.15%), N (0.81%). The factor was mainly composed of galacturonic acid (53% of dry weight) and contained arabinose, fucose, and unidentified component as minor components. The factor also agglutinated A-, B-, AB-, and O types of human erythrocytes to almost the same degree in the presence of Ca2+. The differential spore-agglutinating activity of the factor depended on the pH of the assay medium; it agglutinated similarly the germinated spores of sweet potato and coffee strains at pH 7.5 and 5.5, whereas it displayed a distinct differential agglutinating activity at pH 6.5. The factor was assayed for spore-agglutinating activity at pH ...

1982-02-01

255

Spontaneous mutations affecting the host range of the B77 strain of avian sarcoma virus involve type-specific changes in the virion envelope antigen.  

UK PubMed Central (United Kingdom)

Previously it was shown that the host-range gene of the Bratislava strain of avain sarcoma virus (B77 virus) spontaneously mutates with a very high rate. The wild-type B77 virus called B77 virus-II,...Full Text Available

1977-01-01

256

Real time neutron diffraction experiments using Bragg edges  

Energy Technology Data Exchange (ETDEWEB)

Time resolved phase transition and strain experiments have been performed on the millisecond time scale using a Bragg-edge transmission technique that has been developed at the Los Alamos National Laboratory. The precision with which lattice parameters can be determined from edge positions is sufficient to perform high-resolution strain measurements in uniaxial stress.

1995-12-31

257

Real time neutron diffraction experiments using Bragg edges  

International Nuclear Information System (INIS)

Time resolved phase transition and strain experiments have been performed on the millisecond time scale using a Bragg-edge transmission technique that has been developed at the Los Alamos National Laboratory. The precision with which lattice parameters can be determined from edge positions is sufficient to perform high-resolution strain measurements in uniaxial stress.

1994-12-02

258

Preliminary Assessment of the Safety and Immunogenicity of a New CTX?-Negative, Hemagglutinin/Protease-Defective El Tor Strain as a Cholera Vaccine Candidate  

UK PubMed Central (United Kingdom)

Vibrio cholerae 638 (El Tor, Ogawa), a new CTXΦ-negative hemagglutinin/protease-defective strain that is a cholera vaccine candidate, was examined for safety and immunogenicity...Full Text Available

1999-02-01

259

Organization of lin Genes and IS6100 among Different Strains of Hexachlorocyclohexane-Degrading Sphingomonas paucimobilis: Evidence for Horizontal Gene Transfer  

UK PubMed Central (United Kingdom)

The organization of lin genes and IS6100 was studied in three strains of Sphingomonas paucimobilis (B90A, Sp+, and UT26) which degraded hexachlorocyclohexane...Full Text Available

2004-04-01

260

Open Reading Frame all0601 from Anabaena sp. Strain PCC 7120 Represents a Novel Gene, cnaT, Required for Expression of the Nitrate Assimilation nir Operon  

UK PubMed Central (United Kingdom)

Expression of the nitrate assimilation nir operon in the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 requires the action of both the...Full Text Available

2003-09-01

261

Mechano-transduction in Osteoblastic Cells Involves Strain-regulated Estrogen Receptor ?-mediated Control of Insulin-like Growth Factor (IGF) I Receptor Sensitivity to Ambient IGF, Leading to Phosphatidylinositol 3-Kinase/AKT-dependent Wnt/LRP5 Receptor-independent Activation of ?-Catenin Signaling*  

UK PubMed Central (United Kingdom)

The capacity of bones to adjust their mass and architecture to withstand the loads of everyday activity derives from the ability of their resident cells to respond appropriately to the strains engendered....Full Text Available

2010-03-19

262

Mapping Strain in Nanocrystalline Nitinol: an X-ray Diffraction Method (SULI paper)  

Energy Technology Data Exchange (ETDEWEB)

Understanding the mechanical properties of biomedical devices is critical in predicting and preventing their failure in the body. Such knowledge is essential, for example, in the design of biomedical stents, which must undergo repeated strain over their ten year lifetimes without breaking. Computational models are used to predict mechanical response of a device, but these models are not complete; there are significant deviations from the predictions, especially when devices are subjected to repeated multi-axial loads. Improving these models requires comparisons with actual measurements of strained nitinol. Local measurements of the full strain tensor can be made using X-ray diffraction techniques, but they are currently limited to materials whose grain size is larger than the X-ray beam size or require several diffraction patterns produced by rotation of the sample. Nitinol stents are nanocrystalline, with grains smaller ...

2006-01-04

263

Isolation, sequence, and expression in Escherichia coli of an unusual thioredoxin gene from the cyanobacterium Anabaena sp. strain PCC 7120.  

UK PubMed Central (United Kingdom)

Two sequences with homology to a thioredoxin oligonucleotide probe were detected by Southern blot analysis of Anabaena sp. strain PCC 7120 genomic DNA. One of the sequences was shown to code for a protein...Full Text Available

1989-01-01

264

Induction of an abortive and futile DNA repair process in E. coli by the antitumor DNA bifunctional intercalator, ditercalinium: role in polA in death induction.  

UK PubMed Central (United Kingdom)

Ditercalinium, an antitumor bifunctional intercalator which forms a high affinity reversible complex with DNA, was found to be specifically cytotoxic for polA and lig7 E. coli strains. In the polA strain,...Full Text Available

1988-02-11

265

Fabrication of magnesium alloy (AZ31) sheet by extrusion  

Energy Technology Data Exchange (ETDEWEB)

In order to investigate the extrusion characteristics of magnesium alloy (AZ31), a computer simulation was attempted. Tensile properties of as-cast billet with different strain rates were incorporated into the simulation. The results showed a great change in distribution of stress and strain at near die region by the ram travel. It was found that the average grain size at longitudinal edge of the extruded sheet was smaller than that at center region, which might be attributed to recrystallization caused by severe plastic deformation. (orig.)

2004-07-01

266

Extended-Spectrum-?-Lactamase-Producing Escherichia coli Strains Isolated from Farm Animals from 1999 to 2002: Report from the Japanese Veterinary Antimicrobial Resistance Monitoring Program  

UK PubMed Central (United Kingdom)

A nationwide surveillance for antimicrobial susceptibility in Escherichia coli strains isolated from food-producing animals in Japan was conducted from 1999 to 2002. Eighteen cefazolin-resistant...Full Text Available

2005-08-01

267

Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens.  

UK PubMed Central (United Kingdom)

Fifteen strains of CDC group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. Of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly...Full Text Available

1993-11-01

268

final report on low-cycle fatigue and creep-fatigue testing of steam-filled alloy 800 specimens  

Energy Technology Data Exchange (ETDEWEB)

Uniaxial low-cycle fatigue and creep-fatigue tests have been carried out on hollow alloy 800 specimens that were filled with steam. Two testing temperatures were employed, each with its own steam condition. These temperatures and steam conditions were 650/sup 0/F with saturated steam (5% liquid, 95% vapor) and 1200/sup 0/F with superheated steam at 2200 psi. The low-cycle fatigue tests were carried out at both 650/sup 0/F and 1200/sup 0/F by cycling the strain between equal tensile and compressive magnitudes until specimen failure or until it was no longer practical to continue the test. The creep-fatigue tests were carried out to failure by cycling the strain in the same fashion as in the low-cycle fatigue tests but with holds imposed at either the peak tensile strain or the peak compressive strain or at both peak tensile and compressive strains in each loading cycle.

1981-02-01

269

The influence of martensitic transformation on mechanical properties of cast high alloyed CrMnNi-steel under various strain rates and temperatures  

International Nuclear Information System (INIS)

Metastable austenitic steels show excellent mechanical properties, such as high strength combined with excellent ductility and toughness due to martensitic transformation under mechanical loading (transformation induced plasticity effect). A good energy consumption, and, in the case of high-alloyed metastable austenitic steels, a high corrosion resistance, increase the potential of these materials for diverse applications, also in regard of safety requirements. Up to now, numerous wrought alloys were investigated concerning mechanical behaviour, TRIP-effect, martensitic transformation behaviour and modelling of transformation kinetics or stress-strain behaviour. New high alloyed cast CrMnNi-steels, developed at Technical University Bergakademie Freiberg, provide the chance to reduce processing steps, production time and costs. In order to understand the influence of temperature on the martensitic phase transformation behaviour and therefore on mechanical properties ...

2010-07-01

270

Strain enhanced electron spin polarization observed in photoemission from InGaAs  

International Nuclear Information System (INIS)

Electron spin polarization in excess of 70% has been observed in photoemission from a 0.1 #mu#m-thick epitaxial layer of In_xGa_1_-_xAs with x #approx# 0.13 grown on a GaAs substrate. Under these conditions, the epitaxial layer is expected to be highly strained by the 0.9% lattice mismatch, as confirmed by x-ray diffractometer measurements of the lattice parameter. The electron polarization and the quantum efficiency have been measured as a function of the excitation photon energy from 1.25 to 2.0 eV. A significant enhancement of the electron polarization occurs in the vicinity of 1.33 eV where the expected strain-induced level splitting permits optical excitation of a single band transition. Measurements made on a control sample of 1.14 #mu#m thickness, significantly larger than the critical thickness for pseudomorphic strain, show no polarization enhancement. These measurements represent the first observation of ...

1991-05-06

271

Strain dependence of Si-Ge interdiffusion in epitaxial Si/Si{sub 1-y}Ge{sub y}/Si heterostructures on relaxed Si{sub 1-x}Ge{sub x} substrates  

Science.gov (United States)

The strain dependence of Si-Ge interdiffusion in epitaxial Si/Si{sub 1-y}Ge{sub y}/Si heterostructures on relaxed Si{sub 1-x}Ge{sub x} substrates has been studied using secondary ion mass spectrometry, Raman spectroscopy, and simulations. At 800 and 880 deg. C, significantly enhanced Si-Ge interdiffusion is observed in Si/Si{sub 1-y}Ge{sub y}/Si heterostructures (y=0.56, 0.45, and 0.3) with Si{sub 1-y}Ge{sub y} layers under compressive strain of -1%, compared to those under no strain. In contrast, tensile strain of 1% in Si{sub 0.70}Ge{sub 0.30} layer has no observable effect on interdiffusion in Si/Si{sub 0.70}Ge{sub 0.30}/Si heterostructures. These results are relevant to the device and process design of high mobility dual channel and heterostructure-on-insulator metal oxide semiconductor field effect transistors.

2006-01-02

272

Results of in-house cone-cup testing of low to high temperature SPF-alloys  

Energy Technology Data Exchange (ETDEWEB)

Material testing of SPF-sheet is usually done with coupon hot tensile testing. The optimum range of strain rates is determined with stepped strain tests. To overcome the problem of very few available testing facilities and time consuming and costly procedure, cone cup testing has been developed since long. This test can be generated directly in the workshop, no machining of samples is necessary and it is very much related to the real SPF-process. The analysis of suitable temperature and strain rate is done by simple measurement of resulting cone height. This paper describes a unique and novel variant of the above mentioned baseline design. It provides on-line data of the actual status of the cone cup forming resulting from the applied, constant pressure. Strain rate variations as a result from texture or from the alloy-related behaviour is detectable. Thinning and ...

2004-07-01

273

Nb{sub 3}Sn ITER candidate conductor  

Energy Technology Data Exchange (ETDEWEB)

An internal-tin-process Nb{sub 3}Sn conductor, which is a prototype for the International Thermonuclear Experimental Reactor (ITER), was tested. The axial strain measurements were made over a range of magnetic fields from 15 to 25 T. The conductor specifications are given in Table 5, and the measured data are presented in Table 6 and Figs. 8 through 10. The I{sub c} and J{sub c} values are based on an electric field criterion (E{sub c}) of 2 {mu}V/cm. The results show a zero-strain 15 T value of J{sub c} (referred to the noncopper area) which was 0.50 GA/m{sup 2} and a peak (strain-free) J{sub c} value of 0.55 GA/m{sup 2}. The irreversible strain limit was reasonably high, 0.92%, and the compressive prestrain was 0.28%. The sample did not fracture until 1.09% strain.

1994-01-01

274

Microstructural Coarsening during Thermomechanical Fatigue and Annealing of Micro Flip-Chip Solder Joints  

Energy Technology Data Exchange (ETDEWEB)

Microstructural evolution due to thermal effects was studied in micro solder joints (55 {+-} 5 {micro}m). The composition of the Sn/Pb solder studied was found to be hypereutectic with a tin content of 65--70 wt%.This was determined by Energy Dispersive X-ray analysis and confirmed with quantitative stereology. The quantitative stereological value of the surface-to-volume ratio was used to characterize and compare the coarsening during thermal cycling from 0--160 C to the coarsening during annealing at 160 C. The initial coarsening of the annealed samples was more rapid than the cycled samples, but tapered off as time to the one-half as expected. Because the substrates to which the solder was bonded have different thermal expansion coefficients, the cycled samples experienced a mechanical strain with thermal cycling. The low-strain cycled samples had a 2.8% strain imposed on the solder and failed by 1,000 cycles, despite ...

1998-12-01

275

Isothermal, total strain-controlled cyclic deformation of GGG-60 at temperatures of 20 C {<=}T {<=}500 C. Pt. 1; Isotherme totaldehnungskontrollierte Wechselverformung von GGG-60 im Temperaturbereich 20 C {<=}T {<=}500 C. T. 1  

Energy Technology Data Exchange (ETDEWEB)

The paper examines the influence of various total strain amplitudes and mean strains on the cyclic deformation processes in GGG-60 at temperatures of 20 C, 350 C, and 500 C ,the total strain amplitudes being varied between 1.0 permille {<=}{epsilon}{sub a,t}{<=}3.8 permille , and the mean strains between -4.0 permille {<=}{epsilon}{sub m,t}{<=}4.0 permille . - [Deutsch] In der Arbeit wird der Einfluss unterschiedlicher Totaldehnungsamplituden und Mitteldehnungen auf das Wechselverformungsverhalten von GGG-60 bei Temperaturen von 20 C, 350 C und 500 C untersucht. Dabei werden die Totaldehnungsamplituden zwischen 1,0 permille {<=} {epsilon}{sub a,t} {<=} 3,8 permille und die Mitteldehnungen zwischen -4,0 permille {<=} {epsilon}{sub m,t} {<=} 4,0 permille variiert. (orig./MM)

1995-01-01

276

Fingerprinting using extrolite profiles and physiological data shows sub-specific groupings of Penicillium crustosum strains.  

Science.gov (United States)

Fingerprinting of Penicillium crustosum strains was performed using different phenotypic characteristics. Seven strains of this extremely homogenous species were selected; of these, five originated from geographical locations characterized by low temperatures, and one from a location with a low water activity. Principal component analysis (PCA) was performed using micromorphological data, temperature- and water-dependent growth rates, and extrolite profiles obtained by HPLC analysis. The micromorphological data were less informative, while the growth-rate data were informative only if the strains investigated already showed slight adaptations to the selected external parameter. In contrast, PCA analyses of the extrolite data showed groupings of the strains according to their origins and known physiological differences. These groupings are in full agreement with the clustering obtained by previous ...

2009-04-08

277

Effects of paraquat on Escherichia coli: Differences between B and K-12 strains  

Energy Technology Data Exchange (ETDEWEB)

Escherichia coli B and K-12 are equally susceptible to the bacteriostatic effects of aerobic paraquat, but they differed strikingly when the lethality of paraquat was evaluated. E. coli B suffered an apparent loss of viability when briefly exposed to paraquat, whereas E. coli K-12 did not. This difference depended on the ability of the B-strain, but not the K-12 strain, to retain internalized paraquat; the B strain was killed on aerobic tryptic soy-yeast extract plates during the incubation which preceded the counting of colonies. This difference in retention of paraquat between strains was demonstrated by delayed loss of viability, by growth inhibition, and by cyanide-resistant respiration after brief exposure to paraquat, washing, and testing in fresh medium. This difference was also shown by using ({sup 14}C)paraquat. This previously unrecognized difference between E. coli B and K-12 has been the ...

1990-02-01

278

Anisotropy of tensile properties of extruded magnesium alloy AZ31  

Energy Technology Data Exchange (ETDEWEB)

The mechanical behaviour of wrought magnesium alloy AZ31 has been characterised by tensile testing at room temperature. Tests were carried out at various strain rates between 0.00003 s{sup -1} and 0.01 s{sup -1} on material which had been extruded to a bar of approximately 6 mm thick, and 80 mm wide. Specimens were machined from three orientations relative to the extrusion direction: parallel, perpendicular, and 45 from the extrusion direction. Significant variations of the properties were observed depending on the extrusion orientation and strain rate. At all strain rates the yield stress declined as the test orientation was rotated away from the extrusion direction. Parallel to the extrusion direction, the yield stress was found to increases markedly with increasing strain rate, whereas at 45 and 90 a lesser influence of strain rate was observed. The work hardening exponent was ...

2003-07-01

279

Assimilation of /sup 14/CO/sub 2/ and /sup 14/C sucrose by citrus fruit tissues  

Energy Technology Data Exchange (ETDEWEB)

Assimilation and metabolism of /sup 14/CO/sub 2/ was compared to that of (U-/sup 14/C) sucrose in young grapefruit (ca 25 mm diameter) to determine their respective roles in fruit growth. Fixation of /sup 14/CO/sub 2/ by isolated fruit tissues during 10 min in light exceeded that in dark by 2- to 30-fold depending on tissue content of chlorophyll. Greatest apparent photosynthesis occurred in outer green peel, but green juice tissues assimilated more than did adjoining inner peel tissue. In the dark, juice tissues incorporated 2.5-fold more /sup 14/CO/sub 2/ than any other tissue. Neutral sugars accounted for a smaller proportion and organic acids, a greater proportion, of the /sup 14/C-assimilates in interior peel and juice tissues. These data suggest more extensive production of organic acids from /sup 14/CO/sub 2/ in ...

1987-04-01

280

Role of limited cell replicative capacity in pathological age change. A review  

Energy Technology Data Exchange (ETDEWEB)

Physiological functions are carried out by differentiated cells, with finite lifespans, which age and need to be replaced. In young individuals, tissue functions are sustained at optimal levels because cellular dysfunction and cell loss are balanced by the emergence of newly differentiated cells as stem cells and their partially differentiated descendants replicate. However, with the passage of time the mitotic rates of these cells diminish. Eventually, replications occur too infrequently to offset the loss. It is at this point that the tissue begins to show structural changes and declining function which, as they become pervasive, are identified as ageing. In this paper the theory is set forth that: (1) Diminishing mitotic activity in older tissues results from limited stem cell replicative capacity. (2) All stem cells, regardless of tissue, exhibit similar replicative patterns over time, progressing ...

1982-01-01

281

Elemental composition in mud crab Scylla serrata from Mahanadi estuary, India: in situ irradiation analysis by external PIXE.  

Science.gov (United States)

During the present study concentration of nine elements (K, Ca, Mn, Fe, Cu, Zn, Se, Br and Pb) in different tissues of mud crab Scylla serrata from Mahanadi estuary, India were determined by the external PIXE set up at Institute of Physics, Bhubaneswar, India. The study demonstrates the effectiveness of the technique in analyzing both soft and hard tissue samples from marine organisms and opens the door for non-destructive, multi-elemental analysis of tissue samples with a very little sample preparation by direct irradiation. This technique can be well utilized for analyzing the tissue samples for environmental, toxicological and nutritional purposes. The study also demonstrates the elemental concentrations from tissue samples of any crustaceans from Mahanadi estuary for the first time. Sex based difference in the elemental concentration of the mud crabs were marked, which may be ...

2008-11-01

282

[Method of determining tissue renin activity using heterologous serum].  

Science.gov (United States)

The authors described a method for determination of tissue renin activity with heterologous substrate. The preparation of the substrate was performed at several stages: salting with amonium sulfate; dialisis of the precipitate till complete separation of amonium sulfate molecules; distruction of angiotensinases by interchangeble souring and alcalization of the medium; lyophylization of the pure substrate. The obtained renin-substrate was preserved in ampules and its usage had a series of advantages--duration, economic, a possibility for standartization of the determination, etc., which were described in details in the article. The described in details also the quantitative determination of the renin activity in the tissues (renal and cerebral) with the help of the obtained substrate as the moments, modiied by the authors, were indicated. PMID:436712

1979-01-01

283

[Fundamental biological model for trials of wound ballistics].  

Science.gov (United States)

The aim of our experiment was the testing of effects of common ammunition on usable and slightly accessible biological tissue thereby to create fundamental simple biological model for trials of wounded ballistic. Like objective tissue was elected biological material - pork and beef hind-limbs, pork head, pork bodily cavity. It was discovered that objective tissue is able to react to singles types of shots in all spectrum results namely simple smooth penetration wound as well as splintery fracture in dependence on kind of using ammunition. Pork hind-limb was evaluated like the most suitable biological material for given object. PMID:17172163

2006-10-01

284

Tissue banking for research: connecting the disconnected  

British Library Electronic Table of Contents (United Kingdom)

Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The NHS provides an ideal infrastructure for sample acquisition, but requires two things to make this available?public consent and support for extra manpower and administration. There is a disconnection between the NHS and tissue based research that needs to be addressed on a number of levels to provide a translational platform. This should enable the path to be beaten to provide the ideal tailored treatment for future patients; one that preserves quality of life by curing the dise...

2011-01-01

285

Endomicroscopy imaging of epithelial structures using tissue autofluorescence  

Science.gov (United States)

We explore autofluorescence endomicroscopy as a potential tool for real-time visualization of epithelial tissue microstructure and organization in a clinical setting. The design parameters are explored using two experimental systems--an Olympus Medical Systems Corp. stand-alone clinical prototype probe, and a custom built bench-top rigid fiber conduit prototype. Both systems entail ultraviolet excitation at 266 nm and/or 325 nm using compact laser sources. Preliminary results using ex vivo animal and human tissue specimens suggest that this technology can be translated toward in vivo application to address the need for real-time histology.

2011-04-01

286

Development and utilization of the nominal standard dose for the tolerance dose to sound tissue with radiation t: m1herapy  

International Nuclear Information System (INIS)

The utilization of the Ellis-formula for the determination of the tolerance of normal connective tissue is described. Beyond this, the formula is adapted to so-called critical organs. The individual suppositions implicated by the Ellis-formula are commented. Because of the verifyable congruency of the Ellis-formula with data obtained in clinical practice also by other authors, this conception is presented for the provisional determination of the limits of tolerance of normal tissue together with a computerized programme elaborated for use in different therapeutic techniques. (orig.).

1976-01-01

287

Tumour affinity of [sup 203]Pb-chloride: comparison with [sup 67]Ga-citrate and [sup 201]Tl-chloride  

Energy Technology Data Exchange (ETDEWEB)

[sup 203]Pb-chloride is a promising imaging agent for tumour scanning because of the large retention value for tumour tissue and the small value for normal organs, but the large value for the kidneys and bone is a shortcoming. The retention value of [sup 203]Pb in tumour tissue is larger than that of [sup 201]Tl and smaller than that of [sup 67]Ga. The tumour/inflammatory lesion retention ratio for [sup 203]Pb is very large in comparison with those for [sup 67]Ga and [sup 201]Tl. [sup 203]Pb accumulates to a large extent in viable tumour tissue, and less in necrotic tumour tissue and in inflammatory lesion. (author).

1994-01-01

288

The Importance of Mitochondrial DNA in Aging and Cancer  

UK PubMed Central (United Kingdom)

Mitochondrial dysfunction has been implicated in premature aging, age-related diseases, and tumor initiation and progression. Alterations of the mitochondrial genome accumulate both in aging tissue...Full Text Available

289

Spectral Analysis of Breast Cancer on Tissue Microarrays ...  

Science.gov (United States)

... Although cytologic evaluation of urine specimens is a standard Cesar Angeletti, MD ... 3. Rodriguez-Alonso A, Pita-Fernandez S, Gonzalez-Carrero J, ...

2005-04-01

290

Seamless Integration of Detection and Therapy for Breast ...  

Science.gov (United States)

... that were previously adopted by Zhu [20], Skala [3] and Wang [5,7,8] et al. in their respective studies on epithelial tissue. ...

2007-06-01

291

Regulation of Male Fertility by the Opioid System  

UK PubMed Central (United Kingdom)

Endogenous opioid peptides are substances involved in cell communication. They are present in various organs and tissues of the male and female reproductive tract, suggesting that they may regulate...Full Text Available

2011-07-01

292

Prosthetic rehabilitation of hypophosphatasia: a case report  

UK PubMed Central (United Kingdom)

Hypophosphatasia is a congenital disease characterized by deficiency of serum and tissue non-specific alkaline phosphatase activity. The disease occurs due to mutations in the liver/bone/kidney alkaline...Full Text Available

293

Polyphenols and Aging  

UK PubMed Central (United Kingdom)

Age-associated changes within an individual are inherently complex and occur at multiple levels of organismal function. The overall decline in function of various tissues is known to play a...Full Text Available

2010-02-01

294

Physiological Response in Ovis Aries Resulting from Electrical ...  

Science.gov (United States)

... Secondly, the electrode- tissue interface may have ... stimulation of the optic nerve in a ... Ocular Electronic Vision Prosthesis, Australasian Ophthalmic ...

2001-10-25

295

Pharmacological optimization of tissue perfusion  

UK PubMed Central (United Kingdom)

After fluid resuscitation, vasoactive drug treatment represents the major cornerstone for correcting any major impairment of the circulation. However, debate still rages as to the choice of agent, dose,...Full Text Available

2009-07-01

297

Of Microenvironments and Mammary Stem Cells  

UK PubMed Central (United Kingdom)

In most adult tissues there reside pools of stem and progenitor cells inside specialized microenvironments referred to as niches. The niche protects the stem cells from inappropriate expansion...Full Text Available

2007-06-01

298

Obesity and periodontal disease  

UK PubMed Central (United Kingdom)

Obesity is characterized by the abnormal or excessive deposition of fat in the adipose tissue. Its consequences go far beyond adverse metabolic effects on health, causing an increase in oxidative stress,...Full Text Available

2010-04-01

299

Nuclear magnetic resonance imaging of the abnormal live rat and correlations with tissue characteristics  

Energy Technology Data Exchange (ETDEWEB)

Nuclear magnetic resonance (NMR) images of live rats with sterile and pyogenic abscesses, hematomas, and various implanted and spontaneous neoplasms demonstrated good contrast differentiation between pathologic and surrounding normal tissues. This differentiation was maximal when both the T1 and T2 tissue relaxation times were used as criteria. Neoplasms have a broad range of T1 and T2 values and may be confused with abscesses or hematomas. Tissue rate constants (1/T1 and 1/T2) are mainly dependent on total water content, the exception being fat, which has a 1/T2 value much shorter than that expected on the basis of water content alone.

1981-10-01

300

Novel Cytotoxic Vectors Based on Adeno-Associated Virus  

Wastenet

positive primary PymT breast cancer cells in primary co-cultured tumor tissue, suggesting target specificity of

301

Nicotine and periodontal tissues  

UK PubMed Central (United Kingdom)

Tobacco use has been recognized to be a significant risk factor for the development and progression of periodontal disease. Its use is associated with increased pocket depths, loss of periodontal attachment,...Full Text Available

2010-01-01

302

Manipulation of Nf-KappaB Activity in the Macrophage ...  

Science.gov (United States)

... as far as mammary development is concerned but carries the NF-κB reporter, there is diffuse NF-κB activity throughout the epithelial tissue that is ...

2008-05-01

303

Integration of Tissue-engineered Cartilage With Host Cartilage: An In Vitro Model  

British Library Electronic Table of Contents (United Kingdom)

Background We developed a tissue-engineered biphasic cartilage bone substitute construct which has been shown to integrate with host cartilage and differs from autologous osteochondral transfer in which integration with host cartilage does not occur. Questions/purposes (1) Develop a reproducible in vitro model to study the mechanisms regulating tissue-engineered cartilage integration with host cartilage, (2) compare the integrative properties of tissue-engineered cartilage with autologous cartilage and (3) determine if chondrocytes from the in-vitro formed cartilage migrate across the integration site. Methods A biphasic construct was placed into host bovine osteochondral explant and cultured for up to 8 weeks (n?=?6 at each time point). Autologous osteochondral implants served as controls...

2011-01-01

304

If I Had - Morbid Obesity  

Medline Plus

... for a stapling or division of tissue the creation of a small gastric pouch which then assists ... follow up. Laparoscopic gastric bypass entails the surgical creation of a small gastric pouch, typically about an ...

305

Health and environmental chemistry: Analytical techniques, data management, and quality assurance. Volume 1, Manual  

Energy Technology Data Exchange (ETDEWEB)

Analytical procedures are described for the determination of organic compounds, metals and radioisotopes in environmental materials, human tissues, urine, feces, and waste water.

1993-11-01

306

Guidelines for the Management of Soft Tissue Sarcomas  

UK PubMed Central (United Kingdom)

These guidelines were drawn up following a consensus meeting of UK sarcoma specialists convened under the auspices of the British Sarcoma Group and are intended to provide a framework for the multidisciplinary...Full Text Available

2010-01-01

307

Genetic Architecture Underlying Variation in Extent and Remodeling of the Collateral Circulation  

UK PubMed Central (United Kingdom)

RationaleCollaterals are arteriole-to-arteriole anastomoses that connect adjacent arterial trees. They lessen ischemic tissue injury by serving as endogenous bypass...Full Text Available

2010-08-20

308

Gene Regulation of Extracellular Matrix Remodeling in Human Bone Marrow Stem Cell-Seeded Tissue-Engineered Grafts  

British Library Electronic Table of Contents (United Kingdom)

Tissue-engineered heart valves are prone to early structural deterioration. We hypothesize that cell?scaffold interaction and mechanical deformation results in upregulation of genes related to osteogenic/chondrogenic differentiation and thus changes extracellular matrix (ECM) composition in human bone marrow mesenchymal stem cell (hBMSC)-derived tissue-engineered grafts. hBMSC were expanded and seeded onto poly-glycolic acid/poly-lactic acid scaffold for 14 days. Seeded tissue-engineered constructs (TEC) were subjected to cyclic flexure for 24?h, whereas control TEC was maintained in roller bottles for the same duration. hBMSC, TEC, and mechanically deformed TEC were subjected to gene-array and histological analysis. Expression levels of RNA and/or protein markers related to chondrogenesis...

2011-01-01

309

From Quantitative Microscopy to Automated Image Understanding  

UK PubMed Central (United Kingdom)

Quantitative microscopy has been extensively used in biomedical research and has provided significant insights into structure and dynamics at the cell and tissue level. The entire procedure...Full Text Available

2004-01-01

310

Fluid Transport Phenomena in Ocular Epithelia  

UK PubMed Central (United Kingdom)

This article discusses three largely unrecognized aspects related to fluid movement in ocular tissues; namely, a) the dynamic changes in water permeability observed in corneal and conjunctival...Full Text Available

2008-03-01

311

Expression of cell proliferation and apoptosis biomarkers in pterygia and normal conjunctiva  

UK PubMed Central (United Kingdom)

PurposeTo analyze the expression of apoptosis and cell proliferation molecules in pterygium tissues of Chinese patients.MethodsThirty-three pterygia...Full Text Available

312

Explant culture of gastrointestinal tissue: a review of methods and applications  

British Library Electronic Table of Contents (United Kingdom)

The gastrointestinal (GI) tract is an important target organ for the toxicity of xenobiotics. The toxic effects of xenobiotics on this complex, heterogeneous structure have been difficult to model in vitro and have traditionally been assessed in vivo. The explant culture of GI tissue offers an alternative approach. Historically, the organotypic culture of the GI tract proved far more challenging than the culture of other tissues, and it was not until the late 1960s that Browning and Trier described the means by which intestinal tissues could be successfully cultured. This breakthrough provided a tool researchers could utilise, and adapt, to investigate topics such as the pathogenesis of inflammatory intestinal diseases, the effect of growth factors and cytokines on intestinal proliferation...

2011-01-01

313

Ectopic mineralization of connective tissue in Abcc6-/- mice: effects of dietary modifications and a phosphate binder - a preliminary study  

British Library Electronic Table of Contents (United Kingdom)

Please cite this paper as: Ectopic mineralization of connective tissue in Abcc6-/- mice: effects of dietary modifications and a phosphate binder - a preliminary study. Experimental Dermatology 2008; 17: 203-207. Abstract: Pseudoxanthoma elasticum (PXE), a heritable multisystem disorder, is caused by mutations in the ABCC6 gene. We have developed a murine model for PXE by targeted inactivation of the corresponding mouse gene. A feature of this mouse model is ectopic mineralization of connective tissue capsule surrounding the bulb of vibrissae. This study was designed to investigate the effect of dietary sevelamer hydrochloride (Renagel), a phosphate binder, and specific mineral modifications on ectopic mineralization of connective tissue in Abcc6-/- mice. Three groups were fed a specific di...

2008-01-01

314

Current Projects - Human Nutrition Research Center on Aging ...  

Science.gov (United States)

diet and genetic obesity metabolic defects and inflammation. To determine the role of adipocyte death in promoting adipose tissue inflammation and insulin resistance in animal...

2011-08-31

315

Cancer as a metabolic disease  

UK PubMed Central (United Kingdom)

Emerging evidence indicates that impaired cellular energy metabolism is the defining characteristic of nearly all cancers regardless of cellular or tissue origin. In contrast to normal cells, which...Full Text Available

316

Biodiversity of Vibrios  

UK PubMed Central (United Kingdom)

Vibrios are ubiquitous and abundant in the aquatic environment. A high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals,...Full Text Available

2004-09-01

317

Assessment of the supramolecular structures presents into biological samples by SAXS technique;Avaliacao das estrutras supramoleculares presentes em amostras biologicas atraves da tecnica de SAXS  

Energy Technology Data Exchange (ETDEWEB)

In this work was made an assessment of the supramolecular structures presents into human breast tissue normal and pathological samples, as well as into two types of animals samples (tendon chicken and pork fat) using the small angle x-ray scattering (SAXS) technique. The scattering profiles of the samples were determined at the momentum transfer range O.150nm{sup -1}<=5:q(=4pi.sin({theta}/2)/{lambda})<=8.500nm{sup -1}. In this range, it was possible identify structures corresponding to collagen fibrils (glandular tissue) and to triacylglycerides (adipose tissue) from the correlation between the information extracted from the human breast tissues scattering profiles and those extracted from animals samples. (author)

2009-07-01

318

Adducted proteins for identification of endogenous electrophiles.  

UK PubMed Central (United Kingdom)

Chemically reactive compounds in tissues can be monitored through their products of reaction with biomacromolecules. For the purpose of in vivo dose monitoring, hemoglobin (Hb) has been preferred to...Full Text Available

1993-03-01

319

Adaptive Management of Liver Cancer Radiotherapy  

UK PubMed Central (United Kingdom)

Adaptive radiation therapy for liver cancer has the potential to reduce normal tissue complications and enable dose escalation, allowing the potential for tumor control in this challenging site....Full Text Available

2010-04-01

320

A stem cell niche dominance theorem  

UK PubMed Central (United Kingdom)

BackgroundMultilevelness is a defining characteristic of complex systems. For example, in the intestinal tissue the epithelial lining is organized into crypts that are maintained...Full Text Available

321

A mast cell secretagogue, compound 48/80, prevents the accumulation of hyaluronan in lung tissue injured by ionizing irradiation  

Energy Technology Data Exchange (ETDEWEB)

Irradiation with a single dose of 30 Grey on the basal regions of the lungs of Sprague-Dawley rats induced a peribronchial and alveolar inflammation. Infiltration of mast cells in the edematous alveolar interstitial tissue and also in the peribronchial tissue were characteristic features of the lesion. The appearance of mast cells was already seen 4 wk after irradiation and by weeks 6 to 8 there was a heavy infiltration. The staining properties suggested that they were connective tissue-type mast cells. The infiltration of mast cells was paralleled by an accumulation of hyaluronan (hyaluronic acid) in the alveolar interstitial tissue 6 and 8 wk after irradiation. The recovery of hyaluronan (HA) during bronchoalveolar lavage (BAL) of the lungs also increased at this time. Treatment with a mast cell secretagogue, compound 48/80, induced a distinct reduction of granulated mast cells in the alveolar ...

1990-02-01

322

Strain evolution in Si substrate due to implantation of MeV ion observed by extremely asymmetric x-ray diffraction  

International Nuclear Information System (INIS)

We studied the strain introduced in a Si(111) substrate due to MeV ion implantation using extremely asymmetric x-ray diffraction and measured the rocking curves of asymmetrical 113 diffraction for the Si substrates implanted with a 1.5 MeV Au"2"+ ion at fluence values of 1x10"1"3, 5x10"1"3, and 1x10"1"4/cm"2. The measured curves consisted of a bulk peak and accompanying subpeak with an interference fringe. The positional relationship of the bulk peak to the subpeak and the intensity variation of those peaks with respect to the wavelengths of the x rays indicated that crystal lattices near the surface were strained; the lattice spacing of surface normal (111) planes near the surface was larger than that of the bulk. Detailed strain profiles along the depth direction were successfully estimated using a curve-fitting method based on Darwin's dynamical diffraction theory. Comparing the shapes of resultant ...

2009-08-15

323

The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba  

British Library Electronic Table of Contents (United Kingdom)

The type III secretion system among Gram-negative bacteria is known to deliver effectors into host cell to interfere with host cellular processes. The type III secretion system in Yersina, Pseudomonas and Enterohemorrhagic Escherichia coli have been well documented to be involved in the bacterial pathogenicity. The existence of type III secretion system has been demonstrated in neuropathogenic E. coli K1 strains. Here, it is observed that the deletion mutant of type III secretion system in E. coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain. Next, it was determined whether type III secretion system plays a role in E. coli K1 survival inside Acanthamoeba during the encystment process...

2011-01-01

324

Temperature, moisture, and strain rate effects on the compressive mechanical behavior of Nylon 6/6  

Energy Technology Data Exchange (ETDEWEB)

Material test results are presented for the mechanical behavior of Nylon 66 in compression. Static compression modes include direct compression, stress relaxation and creep. Dynamic direct compression results are included. Tests are performed at atmospheric pressure; strain rates range from 10/sup /minus/4/sec to 10/sup 2/sec; temperatures are 20, 65, 110, 150 and 200/degree/C; moisture levels are 0% (dry), 2% and 6% (saturated); true strains to /minus/0.25 are imposed. Our empirical Temperature-Moisture equivalence of 14/degree/C per 1% moisture is discussed with respect to the /open quotes/free volume/close quotes/ concept.

1988-04-01

325

Stress measurements in welds: Problem areas  

Energy Technology Data Exchange (ETDEWEB)

There have been many stress measurements on welds by neutron diffraction over the past 20 years but there are still a number of serious experimental issues that are often not addressed. The primary fact is that the microstructure generally changes across the weld and accompanying this may be a change in the concentration of strengthening elements in solution. This will lead to a shift in lattice spacing which may be incorrectly interpreted as a strain. Secondly, a gradient of plastic deformation near the weld may be expected. Since plastic deformation by application of a stress always generates intergranular (type-2) strains this may lead to a range of intergranular effects superposed on the conventional weld-related strains. The effects are illustrated by neutron diffraction studies of Zr-4, ferritic and austenitic welds where chemistry, intergranular effects and crystallographic texture can all play a role.

2006-11-10

326

Removal of natural humic acids by decolorizing actinomycetes isolated from different soils (Algeria) for application in water purification  

British Library Electronic Table of Contents (United Kingdom)

Humic acids are considered problematically in drinking water because it can react readily with chlorine to form carcinogen compounds and its biological removal is much recommended. The scanning electron microscopy morphologies and optical parameters observed for natural humic acids (NHAs) extracted from different soils at Mitidja plain (Algeria) made them different from the commercial ones. Three of the most active strains of 19 actinomycetes were isolated and selected from surface soils at this plain. These strains were identified based on cultural characteristics and chemotaxonomic analysis and classified in the genus Streptomyces. Growth of these strains was assured on a poor liquid medium containing NHAs as carbon and nitrogen sources and degradation occur only in the presence of gluco...

2010-01-01

327

Paraquat toxicity is increased in Escherichia coli defective in the synthesis of polyamines  

International Nuclear Information System (INIS)

The authors have shown that toxicity of paraquat for Escherichia coli is increased over 1-fold in strains defective in the biosynthesis of spermidine compared to isogenic strains containing spermidine. The increased sensitivity of these spermidine-deficient mutants to paraquat is eliminated by growth in medium containing spermidine or by endogenous supplementation of spermidine by the use of a speE"+D"+ plasmid. No paraquat toxicity is seen in the absence of oxygen, even in amine-deficient strains, indicating that superoxide is the agent responsible for the increased toxicity. However, the specific mechanisms responsible for the increased paraquat toxicity in the spermidine-deficient mutants remain to be determined. The marked sensitivity to paraquat of E. coli deficient in spermidine is of particular interest, since such mutants have no other phenotypic properties that can be easily assayed. This increased sensitivity has ...

328

Observation of strain-enhanced electron-spin polarization in photoemission from InGaAs  

International Nuclear Information System (INIS)

Electron-spin polarization in excess of 70% has been observed in photoemission from a 0.1-#mu#m-thick epitaxial layer of In_xGa_1_-_xAs with x#approx#0.13 grown on a GaAs substrate. Under these conditions, the epitaxial layer is expected to be highly strained by the 0.9% lattice mismatch. The electron polarization and the quantum efficiency have been measured as a function of the excitation photon energy from 1.25 to 2.0 eV. A significant enhancement of the electron polarization occurs in the vicinity of 1.33 eV where the expected strain-induced level splitting permits optical excitation of a single-band transition.

329

Observation of dislocation-mediated layer-by-layer interface growth  

Energy Technology Data Exchange (ETDEWEB)

The growth of thin Pd[sub 2]Si films on Si(111) surfaces is studied using [ital in] [ital situ] transmission electron microscope under ultrahigh vacuum conditions. No immediate reaction of deposited Pd with Si is observed at room temperature. At [similar to]200 [degree]C, uniform Pd[sub 2]Si films can be formed. The thin Pd[sub 2]Si films are found to grow into strained islands at elevated temperatures. Interfacial misfit dislocations associated with interfacial steps propagate across the strained islands, causing the islands to grow layer-by-layer at the interface. The strain fields associated with the misfit dislocations are believed to be responsible for this behavior.

1994-07-11

330

Nonlinear dynamic analysis of high energy line pipe whip  

Energy Technology Data Exchange (ETDEWEB)

This paper describes a nonlinear dynamic analysis of TVA high energy line pipe whip tests using the ABAQUS-EPGEN code. The analysis considers the effects of large deformation and strain rate on resisting moment and energy absorption capability. The numerical results of impact forces, impact velocities, pipe strains, and reaction forces at pipe supports are compared to the TVA test data. The calculated pipe whip impact time and forces are also compared with those predicted using current industry practice. The calculated pipe support reaction forces are found to be in good agreement with the TVA test data except for some peak values at the very beginning of the pipe break. These peaks are believed to be due to stress wave propagation which cannot be addressed by the ABAQUS code. Both elbow crushing and strain rate have been approximately simulated. The effects are found to be important for pipe whip impact evaluation.

1984-02-01

331

Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium  

British Library Electronic Table of Contents (United Kingdom)

Abstract Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and...

2008-01-01

332

Investigation of lattice strains in layered structures containing porous silicon  

International Nuclear Information System (INIS)

Silicon layered structures containing porous silicon modified with various thermal treatments and epitaxial layers deposited on porous layers were studied with a number of complementary X-ray diffraction methods using synchrotron source. The methods of characterization included recording of rocking curves for reflections with various asymmetry as well as projection, section and micro-Laue topography. It was found that oxidizing and sintering of porous silicon seriously modified the strains in the porous layer and in some cases even inverting the sense of strain with respect to that in initially formed porous layer. Consequently the deposited epitaxial layer usually was not laterally coherent with the substrate. Some of investigated layers were not stable in time and after few months period exhibited significant lost of coherence of porous skeleton. (author)

2001-09-23

333

Internal strain and texture evolution during deformation twinning in magnesium  

Energy Technology Data Exchange (ETDEWEB)

The development of a twinned microstructure in hexagonal close-packed rolled magnesium compressed in the in-plane direction has been monitored in situ with neutron diffraction. The continuous conversion of the parent to daughter microstructure is tracked through the variation of diffraction peak intensities corresponding to each. Approximately 80% of the parent microstructure twins by 8% compression. Elastic lattice strain measurements indicate that the stress in the newly formed twins (daughters) is relaxed relative to the stress field in the surrounding matrix. However, since the daughters are in a plastically 'hard' deformation orientation, they quickly accumulate elastic strain as surrounding grains deform plastically. Polycrystal modeling of the deformation process provides insight about the crystallographic deformation mechanism involved.

2005-06-15

334

Intermediate Strain-Rate Loading Experiments - Technique and Applications to Ceramics  

Energy Technology Data Exchange (ETDEWEB)

A new test methodology is described which allows access to loading rates that lie between split Hopkinson bar and shock-loading techniques. Gas gun experiments combined with velocity interferometry techniques have been used to experimentally determine the intermediate strain-rate loading behavior of Coors AD995 alumina and Cercom silicon-carbide rods. Graded-density materials have been used as impactors; thereby eliminating the tension states generated by the radial stress components during the loading phase. Results of these experiments demonstrate that the time-dependent stress pulse generated during impact allows an efficient transition from the initial uniaxial strain loading to a uniaxial stress state as the stress pulse propagates through the rod. This allows access to intermediate loading rates over 5 x 10{sup 3}/s to a few times 10{sup 4}/s.

1999-08-16

335

Interlattice displacements and elastic constants of the A-15 compounds V_3Si, V_3Ge and Nb_3Sn  

International Nuclear Information System (INIS)

General expressions for the interlattice displacements of the A-15 structure compounds are obtained in terms of the strain components making use of the deformation theory. The nature of the interlattice displacements of all the 8 atoms in the unit cell is discussed. It is found that the interlattice displacements occur in such a way that the pair of atoms along any linear chain move in opposite directions with equal magnitudes. Expression for the strain energy of these compounds is developed using deformation theory and this is compared with the strain energy expression from continuum theory to obtain the elastic constants. The theoretical values of the elastic constants fairly agree with the experimental values for V_3Si, V_3Ge and Nb_3Sn. (author).

336

Interlattice displacements and elastic constants of the A-15 compounds V3Si, V3Ge and Nb3Sn  

Science.gov (United States)

General expressions for the interlattice displacements of the A-15 structure compounds are obtained in terms of the strain components making use of the deformation theory. The nature of the interlattice displacements of all the 8 atoms in the unit cell is discussed. It is found that the interlattice displacements occur in such a way that the pair of atoms along any linear chain move in opposite directions with equal magnitudes. Expression for the strain energy of these compounds is developed using deformation theory and this is compared with the strain energy expression from continuum theory to obtain the elastic constants. The theoretical values of the elastic constants fairly agree with the experimental values for V3Si, V3Ge and Nb3Sn.

1978-03-01

337

Initial grain size effect of dynamic recrystallization of magnesium alloy AZ31  

Energy Technology Data Exchange (ETDEWEB)

Dynamic development of new grain boundaries was studied in compression of a magnesium alloy AZ31 with initial grain sizes (D{sub 0}) of 22 {mu}m and 90 {mu}m at a temperature of 673 K. Kink bands are evolved near corrugated grain boundaries and in grain interiors at low strains accompanied with new fine grains. Kink bands are developed often perpendicular to the basal plane. The boundary misorientation of kink band as well as the volume fraction of new grains increases rapidly with increasing strain and approaches a saturation value in high strain. New fine grains are developed faster with decrease in the D{sub 0}. It is concluded that new grain evolution is controlled by a deformation-induced continuous reaction, i.e. continuous dynamic recrystallization (DRX). (orig.)

2003-07-01

338

Hardening effect in Ti-3Al-2.5V during high temperature deformation  

International Nuclear Information System (INIS)

High temperature deformation behavior of an alpha + beta titanium alloy Ti-3Al-2.5V has been investigated in the temperature range 830 deg. C to 910 deg. C by using the method of cross head speed cycling. On the basis of flow stress-strain rate and strain rate sensitivity data, it was found that the alloy exhibits a hardening effect in the entire temperature range studied, amount of hardening being significant at the highest test temperature of 910 deg. C which resulted in a degradation of superplastic properties of the alloy. Grain growth in beta-phase due to strain was considered responsible for the hardening effect observed.

339

Growth and defects of explosives crystals  

Energy Technology Data Exchange (ETDEWEB)

Large single crystals of PETN, RDX, and TNT can be grown easily from evaporating ethyl acetate solutions. The crystals all share a similar type of defect that may not be commonly recognized. The defect generates conical faces ideally mosaic crystals, and may account for the polymorphs'' of TNT and detonator grades of PETN. TATB crystals manufactured by the amination of trichlorotrinitrobenzene in dry toluene entrain two forms of ammonium chloride. One of these forms causes worm holes'' in the TATB crystals that may be the reason for its unusually low failure diameters. Strained HMX crystals form mechanical twins that can spontaneously revert back to the untwinned form when the straining force is removed. Large strains or temperatures above 100[degrees]C lock in the mechanical twins.

1992-01-01

340

Growth and defects of explosives crystals  

Energy Technology Data Exchange (ETDEWEB)

Large single crystals of PETN, RDX, and TNT can be grown easily from evaporating ethyl acetate solutions. The crystals all share a similar type of defect that may not be commonly recognized. The defect generates conical faces ideally mosaic crystals, and may account for the ``polymorphs`` of TNT and detonator grades of PETN. TATB crystals manufactured by the amination of trichlorotrinitrobenzene in dry toluene entrain two forms of ammonium chloride. One of these forms causes ``worm holes`` in the TATB crystals that may be the reason for its unusually low failure diameters. Strained HMX crystals form mechanical twins that can spontaneously revert back to the untwinned form when the straining force is removed. Large strains or temperatures above 100{degrees}C lock in the mechanical twins.

1992-12-01

341

Glutathione peroxidase activity in the selenium-treated alga Scenedesmus quadricauda  

British Library Electronic Table of Contents (United Kingdom)

The function of selenium in an organism is mediated mostly by selenoproteins including glutathione peroxidase. Glutathione peroxidase is a potent anti-oxidative enzyme, scavenging a variety of peroxides. The green alga Scenedesmus quadricauda was used to investigate the relationship between the toxicity of selenium and the glutathione peroxidase activity. Selenium resistant strains SeIV and SeVI were synchronized and grown in high concentrations of Se (selenite or selenate). As a measure of selenium toxicity the EC50 values were determined. During growth of the untreated wild type, glutathione peroxidase activity increased slightly and then declined gradually until the end of the cell cycle. A similar pattern was observed in untreated resistant strains and when resistant strains were grown...

2011-01-01

342

Genomic survey of prepulse inhibition in mouse chromosome substitution strains  

British Library Electronic Table of Contents (United Kingdom)

Prepulse inhibition (PPI) is a measure of sensorimotor gating, a pre-attentional inhibitory brain mechanism that filters extraneous stimuli. Prepulse inhibition is correlated with measures of cognition and executive functioning, and is considered an endophenotype of schizophrenia and other psychiatric illnesses in which patients show PPI impairments. As a first step toward identifying genes that regulate PPI, we performed a quantitative trait locus (QTL) screen of PPI phenotypes in a panel of mouse chromosome substitution strains (CSSs). We identified five CSSs with altered PPI compared with the host C57BL/6J strain: CSS-4 exhibited decreased PPI, whereas CSS-10, -11, -16 and -Y exhibited higher PPI compared with C57BL/6J. These data indicate that A/J chromosomes 4, 10, 11, 16 and Y harbor...

2009-01-01

343

Experimental and analytical studies of four-inch pipe whip tests under PWR LOCA conditions  

Energy Technology Data Exchange (ETDEWEB)

This paper presents experimental and analytical results of pipe whip tests performed under PWR LOCA conditions using a test pipe of 4-inch diameter and U-shaped restraints. In the tests, the effects of the overhang length on the pipe whip behavior of the piperestraints system were studied by measuring the strains and deformations of the test pipe and restraints, and the restraints forces. The equation for predicting the maximum strain at the outer surface of the pipe was derived using a static equilibrium condition. The calculated maximum strains at the outer surface of the pipe agree fairly well with experimental data. The dynamic response analysis of the pipe-restraints system was conducted by the finite element program ADINA. The applicability of the ADINA program to the pipe whip analysis is made clear through this analysis.

1984-01-01

344

Evolution of lattice strain in Ti-6Al-4V during tensile loading at room temperature  

International Nuclear Information System (INIS)

The evolution of intergranular lattice strains in a textured, forged bar (Bar) sample of the #alpha# - #beta# titanium alloy Ti-6Al-4V has been characterised using in situ X-ray diffraction. A two-phase elastic-plastic self-consistent (EPSC) model has been developed to rationalise the results. Of the orientations analysed, it is found that the #left brace#2 0 0#right brace# #beta# orientation is the most compliant and that load partitions to this orientation during plasticity. The results from the bar material have then been used to predict the response of unidirectionally rolled plate (UD) Ti-6Al-4V. It is predicted that the residual lattice strains in the #left brace#101-bar0#right brace# and #left brace#112-bar0#right brace# orientations will be significantly higher in the UD material.

2008-12-01

345

Evidence for Epigenetic Interactions for Loci on Mouse Chromosome 1 Regulating Open Field Activity  

British Library Electronic Table of Contents (United Kingdom)

The expression of motor activity levels in response to novel situations is under complex genetic and environmental control. Several genetic loci have been implicated in the regulation of this behavioral phenotype, but their relationship to epigenetic and epistatic interactions is relatively unknown. Here, we report on a quantitative trait locus (QTL) on mouse chromosome 1 for novelty-induced motor activity in the open field, using chromosome substitution strains derived from a high active host strain (C57BL/6J) and a low active donor strain (A/J). The QTL for open field (horizontal distance moved) peaked at the location of Kcnj9, however, QTL detection was initially masked by an interplay of both grandparent genetic origin and genetic co-factors influencing behavior on chromosome 1. Our fi...

2009-01-01

346

DNase-resistant transfer of chromosomal cat and tet insertions by filter mating in pneumococcus  

Energy Technology Data Exchange (ETDEWEB)

Genes for chloramphenicol resistance, Cm(r) and tetracycline resistance, Tc(r), which are present as heterologous insertions in the chromosomes of some clinical isolates of Streptococcus pneumoniae (pneumococcus) and derivative strains, were transferred at a low frequency to other pneumococci by a DNase-resistant filter mating process that resembles conjugation. Cotransfer of Cm(r) and Tc(r) was the most common event. Neither the donor strains nor the transconjugants contained detectable plasmids. Transconjugants acted as donors for transformation and for filter mating and had properties similar to those of the parent strain. The presence of the conjugative plasmid pIP501 in the donor did not appear to influence the transfer properties of the Cm(r) or Tc(r) determinants.

1980-01-01

347

A study on the transient analysis of pipe and restraint due to impact loading  

Energy Technology Data Exchange (ETDEWEB)

In this study, experiments were performed for pipe whip phenomena simulation. The analytical method was developed by combining several kinds of elements in ABAQUS computer code, standard version 5.2 . The transient analytical and the experimental results of the pipe whip test using 6 inch diameter pipe and U-shaped restraints are presented. It is shown that the adequate clearance decreases the restraint strains. As the overhang length increased, the maximum strain of restraints decreased. When the impact force increased, the maximum restraint strain increased slightly. The analytical models simulate the experimental impact phenomena very precisely, with slight conservatism. It is verified that the analytical method developed is very suitable to pipe whip phenomena analysis in nuclear power plants. 6 refs., 10 figs., 1 tab.

1995-12-31

348

Tissue structure, nuclear organization and gene expression in normal and malignant breast  

Energy Technology Data Exchange (ETDEWEB)

Because every cell within the body has the same genetic information, a significant problem in biology is to understand how cells within a tissue express genes selectively. A sophisticated network of physical and biochemical signals converge in a highly orchestrated manner to bring about the exquisite regulation that governs gene expression in diverse tissues. Thus, the ultimate decision of a cell to proliferate, express tissue-specific genes, or apoptose must be a coordinated response to its adhesive, growth factor, and hormonal milieu. The unifying hypothesis examined in this overview is that the unit of function in higher organisms is neither the genome nor the cell alone but the complex, three-dimensional tissue. This is because there are bidirectional connections between the components of the cellular microenvironment (growth factors, hormones, and extracellular matrix) and the nucl2048 These ...

2000-01-27

349

In-situ measurement of epithelial tissue optical properties: Development and implementation of diffuse reflectance spectroscopy techniques  

Science.gov (United States)

Cancer is a severe threat to human health. Early detection is considered the best way to increase the chance for survival. While the traditional cancer detection method, biopsy, is invasive, noninvasive optical diagnostic techniques are revolutionizing the way that cancer is diagnosed. Reflectance spectroscopy is one of these optical spectroscopy techniques showing promise as a diagnostic tool for pre-cancer detection. When a neoplasia occurs in tissue, morphologic and biochemical changes happen in the tissue, which in turn results in the change of optical properties and reflectance spectroscopy. Therefore, a pre-cancer can be detected by extracting optical properties from reflectance spectroscopy. This dissertation described the construction of a fiberoptic based reflectance system and the development of a series of modeling studies. This research is aimed at establishing an improved understanding of the optical properties of mucosal ...

2009-01-01

350

tA Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta  

UK PubMed Central (United Kingdom)

The rice blast resistance (R) gene Pi-ta mediates gene-for-gene resistance against strains of the fungus Magnaporthe grisea that express avirulent...Full Text Available

2000-11-01

351

Vibrio cholerae Hemagglutinin/Protease Inactivates CTX?  

UK PubMed Central (United Kingdom)

Pathogenic strains of Vibrio cholerae are lysogens of the filamentous phage CTXφ, which carries the genes for cholera toxin (ctxAB). We found that the titers...Full Text Available

1998-09-01

352

Variations in ventral root axon morphology and locomotor behavior components across different inbred strains of mice  

British Library Electronic Table of Contents (United Kingdom)

Locomotion is a complex behavior affected by many different brain- and spinal cord systems, as well as by variations in the peripheral nervous system. Recently, we found increased gene expression for EphA4, a gene intricately involved in motor neuron development, between high-active parental strain C57BL/6J and the low-active chromosome substitution strain 1 (CSS1). CSS1 mice carry chromosome 1 from A/J mice in a C57BL/6J genetic background, allowing localization of quantitative trait loci (QTL) on chromosome 1. To find out whether differences in motor neuron anatomy, possibly related to the changes in EphA4 expression, are involved in the motor activity differences observed in these strains, motor performance in various behavioral paradigms and anatomical differences in the ventral roots ...

2009-01-01

353

Variation in Nicotine Consumption in Inbred Mice Is Not Linked to Orosensory Ability  

UK PubMed Central (United Kingdom)

Genetic studies of nicotine addiction in mice have utilized the oral self-administration model. However, it is unclear if strain differences in nicotine consumption are influenced by variation in bitter...Full Text Available

2009-01-01

354

Transport and deamination of amino acids by a gram-positive, monensin-sensitive ruminal bacterium.  

UK PubMed Central (United Kingdom)

Strain F, a recently isolated ruminal bacterium, grew rapidly with glutamate or glutamine as an energy source in the presence but not the absence of Na. Monensin, a Na+/H+ antiporter, completely inhibited...Full Text Available

1990-07-01

355

Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum.  

UK PubMed Central (United Kingdom)

Rhizobium leguminosarum, biovar viceae, strain RCC1001 contains two glutamine synthetase activities, GSI and GSII. We report here the identification of glnA, the structural gene for GSI. A 2 kb fragment...Full Text Available

1987-03-11

356

The Zinc Finger SET Domain Gene Prdm14 Is Overexpressed in Lymphoblastic Lymphomas with Retroviral Insertions at Evi32  

UK PubMed Central (United Kingdom)

BackgroundAKXD recombinant inbred strains of mice have proven to be very useful in the identification of potential oncogenes and tumor suppressors involved in the development of...Full Text Available

357

Technical approaches for mouse models of human disease  

UK PubMed Central (United Kingdom)

The mouse is the leading organism for disease research. A rich resource of genetic variation occurs naturally in inbred and special strains owing to spontaneous mutations. However, one can also obtain...Full Text Available

2011-05-01

358

Susceptibility of the "penicillinase-resistant" penicillins and cephalosporins to penicillinase of Staphylococcus aureus.  

UK PubMed Central (United Kingdom)

The activities of some semisynthetic penicillins and cephalosporins have been tested against clinical strains of Staphylococcus aureus. The apparent activity in vitro varies with the method of testing...Full Text Available

1977-01-01

359

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1  

UK PubMed Central (United Kingdom)

Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism...Full Text Available

1974-09-01

360

Succession of Indigenous Pseudomonas spp. and Actinomycetes on Barley Roots Affected by the Antagonistic Strain Pseudomonas fluorescens DR54 and the Fungicide Imazalil  

UK PubMed Central (United Kingdom)

In recent years, the interest in the use of bacteria for biological control of plant-pathogenic fungi has increased. We studied the possible side effects of coating barley seeds with the antagonistic...Full Text Available

2001-03-01

361

Spread of a highly mucoid Streptococcus pyogenes emm3/ST15 clone  

UK PubMed Central (United Kingdom)

BackgroundHyaluronic acid capsule plays a key role in Streptococcus pyogenes virulence. Circulation of mucoid or highly encapsulated strains has been related to...Full Text Available

362

Spread of a Salmonella typhimurium Clone Resistant to Expanded-Spectrum Cephalosporins in Three European Countries  

UK PubMed Central (United Kingdom)

Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance...Full Text Available

1999-11-01

363

Spoligotype Profile of Mycobacterium tuberculosis Complex Strains from HIV-Positive and -Negative Patients in Nigeria: a Comparative Analysis ?  

UK PubMed Central (United Kingdom)

We ran a comparative analysis of all patients for whom a positive culture of Mycobacterium tuberculosis complex was available between April 2004 and October 2005 and whose HIV serology...Full Text Available

2011-01-01

364

Social Deficits, Stereotypy, and Early Emergence of Repetitive Behavior in the C58/J Inbred Mouse Strain  

UK PubMed Central (United Kingdom)

Mouse lines with behavioral phenotypes relevant to symptoms in neurodevelopmental disorders may provide models to test hypotheses about disease etiology and to evaluate potential treatments....Full Text Available

2010-03-17

365

Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis  

UK PubMed Central (United Kingdom)

BackgroundThe Streptococcus pyogenes (group A streptococci, GAS) two-component signal transduction system CovRS has been described to be important for pathogenesis...Full Text Available

366

Septum Formation in Escherichia coli: Characterization of Septal Structure and the Effects of Antibiotics on Cell Division  

UK PubMed Central (United Kingdom)

Septa can be demonstrated in sections of Escherichia coli strains B and B/r after fixation with acrolein and glutaraldehyde. The septum consists of an ingrowth of the cytoplasmic membrane...Full Text Available

1974-07-01

367

Salmonella enterica in Commercial Swine Feed and Subsequent Isolation of Phenotypically and Genotypically Related Strains from Fecal Samples?  

UK PubMed Central (United Kingdom)

The purpose of this study was to determine the occurrence and genotypic relatedness of Salmonella enterica isolates recovered from feed and fecal samples in commercial swine production...Full Text Available

2010-11-01

368

Safety and immunogenicity of a vaccine bait containing ERA strain of attenuated rabies virus.  

UK PubMed Central (United Kingdom)

Ninety percent of foxes fed commercial ERA vaccine in a specially designed bait developed rabies serum neutralizing antibodies. The vaccine bait did not cause clinical signs of rabies when consumed...Full Text Available

1987-10-01

369

Role of sodium in the growth of a ruminal selenomonad.  

UK PubMed Central (United Kingdom)

The ruminal selenomonad strain H18 grew rapidly (mu = 0.50 h-1) in a defined medium containing glucose, ammonia, purified amino acids, and sodium (95 mM); little if any ammonia was utilized as a nitrogen...Full Text Available

1991-06-01

370

Role of copper resistance in competitive survival of Pseudomonas fluorescens in soil.  

UK PubMed Central (United Kingdom)

A copper-resistant strain (09906) of Pseudomonas fluorescens that was isolated from a citrus grove soil is being investigated as a biological control agent for Phytophthora root rot. Since citrus grove...Full Text Available

1993-02-01

371

Role of Group A Streptococcal Virulence Factors in Adherence to Keratinocytes  

UK PubMed Central (United Kingdom)

To evaluate the role of putative group A streptococcal virulence factors in the initiation of skin infections, we compared the adherence of a wild-type M49-protein skin-associated strain to that of...Full Text Available

2000-03-01

372

Recovery of Pathogenic Measles Virus from Cloned cDNA  

UK PubMed Central (United Kingdom)

Reverse genetics technology so far established for measles virus (MeV) is based on the Edmonston strain, which was isolated several decades ago, has been passaged in nonlymphoid cell lines, and is no...Full Text Available

2000-07-01

373

Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication  

UK PubMed Central (United Kingdom)

Human cytomegalovirus (HCMV) in clinical material cannot replicate efficiently in vitro until it has adapted by mutation. Consequently, wild-type HCMV differ fundamentally from the passaged strains...Full Text Available

2010-09-01

374

QTL analysis of measures of mouse home-cage activity using B6/MSM consomic strains  

UK PubMed Central (United Kingdom)

The activity of mice in their home cage is influenced greatly by the cycle of light and dark. In addition, home-cage activity shows remarkable time-dependent changes that result in a prominent temporal...Full Text Available

2010-10-01

375

Production, Purification, and Characterization of ?-Galactosidase from Monascus pilosus  

UK PubMed Central (United Kingdom)

A Monascus pilosus strain was selected for production of intracellular α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Galactose...Full Text Available

1986-11-01

376

Prevalence and Characterization of Human Shigella Infections in Henan Province, China, in 2006 ?  

UK PubMed Central (United Kingdom)

In 2006, 3,531 fecal samples were collected from patients with diarrhea in Henan Province, China. A total of 467 (13.2%) Shigella strains were isolated and serotyped. Seventy-one...Full Text Available

2011-01-01

377

Plane-strain backward extrusion of AZ31 magnesium alloy  

Energy Technology Data Exchange (ETDEWEB)

Increase usage of magnesium components in the portable electronics industries has propelled considerable research interest on the development of magnesium forging. There have been some studies done in the area of sheet metal forming and forging of axis-symmetrical parts of magnesium alloys but there are very little work done on the plane strain backward extrusion of magnesium alloy. This paper deals with the analysis of plane strain extrusion of magnesium alloy, AZ31, using numerical modeling at different extrusion conditions of temperatures and friction coefficients. A commercially available finite element package, ANSYS/LSDYNA, was used in the finite element analysis. FE simulated results, material deformation, punch force and stress/strain distributions, were compared with actual experimental data. From the numerical analysis, a decrease in forging temperature from 300 C to 250 C will lead to a moderate increase in ...

2003-07-01

378

Phylogenetic and Evolutionary Relationships among Yellow Fever Virus Isolates in Africa  

UK PubMed Central (United Kingdom)

Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have...Full Text Available

2001-08-01

379

Phagocytosis of Campylobacter jejuni and its intracellular survival in mononuclear phagocytes.  

UK PubMed Central (United Kingdom)

In vitro phagocytosis and intracellular survival of Campylobacter jejuni strain 2964 in mononuclear phagocytes were studied. The following three types of mononuclear phagocytes were used: a J774G8 peritoneal...Full Text Available

1985-05-01

380

Persistence of genetic variants of the arctic fox strain of Rabies virus in southern Ontario  

UK PubMed Central (United Kingdom)

AbstractGenetic-variant analysis of rabies viruses provides the most sensitive epidemiologic tool for following the spread and persistence of these viruses in their wildlife hosts. Since...Full Text Available

2006-01-01

381

Pathogenesis of the Human Opportunistic Pathogen Pseudomonas aeruginosa PA14 in Arabidopsis1  

UK PubMed Central (United Kingdom)

The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 is a multihost pathogen that can infect Arabidopsis. We found that PA14 pathogenesis in Arabidopsis involves the...Full Text Available

2000-12-01

382

Partial purification and characterization of the multiple molecular forms of staphylococcal clotting activity (coagulase).  

UK PubMed Central (United Kingdom)

The clotting activity of Staphylococcus aureus strain 104 was purified 46,000-fold, but absolute purity was not achieved. Carbohydrate content of the purified material was not more than 5%. Elution...Full Text Available

1981-12-01

383

Organization of Bacteriophage Tail-Like Particles in Cells of Chromobacterium violaceum  

UK PubMed Central (United Kingdom)

A strain of Chromobacterium violaceum has been isolated which produces bacteriophage tail-like particles in high numbers. The extracellular morphology and the intracellular arrangement...Full Text Available

1972-05-01

384

O2-dependent methionine auxotrophy in Cu,Zn superoxide dismutase-deficient mutants of Saccharomyces cerevisiae.  

UK PubMed Central (United Kingdom)

Mutant strains of the yeast Saccharomyces cerevisiae which lack functional Cu,Zn superoxide dismutase (SOD-1) do not grow aerobically unless supplemented with methionine. The molecular basis of this...Full Text Available

1990-04-01

385

Novel Cefotaximase (CTX-M-16) with Increased Catalytic Efficiency Due to Substitution Asp-240?Gly  

UK PubMed Central (United Kingdom)

Three clinical strains (Escherichia coli Rio-6, E. coli Rio-7, and Enterobacter cloacae Rio-9) collected in 1996 and 1999 from hospitals in Rio de...Full Text Available

2001-08-01

386

Nitrogen metabolite repression of nitrate reductase in Neurospora crassa.  

UK PubMed Central (United Kingdom)

The effect of different nitrogen compounds on the induction of reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase was examined in Neurospora crassa. Whereas in the wild-type strain...Full Text Available

1979-03-01

387

Nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia.  

UK PubMed Central (United Kingdom)

Forty-eight mutants unable to reduce nitrate were isolated from "cowpea" Rhizobium sp. strain 32Hl and examined for nitrogenase activity in culture. All but two of the mutants had nitrogenase activity...Full Text Available

1977-02-01

388

Multivariate analyses reveal common and drug specific genetic influences on responses to four drugs of abuse  

UK PubMed Central (United Kingdom)

Vulnerability to abused drugs is influenced by multiple genes unique to each drug as well as to risk genes for polydrug abuse. If several inbred mouse strains respond to different drugs similarly,...Full Text Available

2008-11-01

389

Multiplicity of Plasmodium falciparum infection in asymptomatic children in Senegal: relation to transmission, age and erythrocyte variants  

UK PubMed Central (United Kingdom)

BackgroundIndividuals living in malaria endemic areas generally harbour multiple parasite strains. Multiplicity of infection (MOI) can be an indicator of immune status. However,...Full Text Available

390

Molecular cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.  

UK PubMed Central (United Kingdom)

This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...Full Text Available

1989-11-01

391

Molecular characterization of hepatitis A virus isolates from environmental and clinical samples in Greece  

UK PubMed Central (United Kingdom)

BackgroundHepatitis A virus (HAV) strains detected in environmental and clinical samples were analysed to characterize the genotypes of HAV circulating in Greece. Fifty (50) sewage...Full Text Available

392

Molecular biological enhancement of coal biodesulfurization. Final report, October 1988--December 1991  

Energy Technology Data Exchange (ETDEWEB)

The overall objective of this project was to use molecular genetics to develop strains of bacteria with enhanced ability to remove sulfur from coal, and to obtain data that will allow the performance and economics of a coal biodesulfurization process to be predicted. (VC)

1991-12-01

393

Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolated from the Eye  

UK PubMed Central (United Kingdom)

Purpose:Methicillin-resistant Staphylococcus aureus (MRSA) strains are commonly classified as hospital-acquired (HA) or community-acquired (CA). Typical HA-MRSA...Full Text Available

2011-02-01

394

Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum ?-Lactamases Isolated in Rome, Italy?  

UK PubMed Central (United Kingdom)

Escherichia coli strains producing extended-spectrum β-lactamases (ESBLs) are a major problem in many different hospitals worldwide, causing outbreaks as well as sporadic infections....Full Text Available

2008-01-01

395

Mechanical Properties of Cellular Materials  

Energy Technology Data Exchange (ETDEWEB)

The authors calculated the stress-strain relation for elastomeric foam from an ab initio theory, which shows that the plateau and densification regions should be described by a hyperbola. The theory seems to agree reasonably well with experiment.

1999-07-09

396

Lizards in the ecology of salmonellosis in Panama.  

UK PubMed Central (United Kingdom)

Enteropathogenic bacteria was isolated from 131 of 447 (29.4%) neotropical Panamanian lizards belonging to 34 species of seven families. Overall, 147 strains of bacteria were isolated comprising 26...Full Text Available

1981-05-01

397

Legionella tucsonensis sp. nov. isolated from a renal transplant recipient.  

UK PubMed Central (United Kingdom)

A Legionella-like organism (strain 1087-AZ-H) was isolated from a pleural-fluid specimen from a renal transplant patient undergoing immunosuppressive therapy. Growth characteristics and gas-liquid chromatography...Full Text Available

1989-08-01

398

Legionella lansingensis sp. nov. isolated from a patient with pneumonia and underlying chronic lymphocytic leukemia.  

UK PubMed Central (United Kingdom)

A Legionella-like organism, strain 1677-MI-H, was isolated from the bronchoscopy washings of a patient with pneumonia who had a 2-year history of progressive, chronic lymphocytic leukemia. The growth...Full Text Available

1992-09-01

399

Legionella fairfieldensis sp. nov. isolated from cooling tower waters in Australia.  

UK PubMed Central (United Kingdom)

Three Legionella-like organisms were isolated from water from the cooling towers of two Australian institutions. The strains grew on buffered charcoal-yeast extract (BCYE) agar but not on BCYE agar...Full Text Available

1991-03-01

400

Large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes.  

UK PubMed Central (United Kingdom)

A single large plasmid was isolated from multiplasmid-harboring strains Rhizobium leguminosarum 1001 and R. trifolii 5. These single plasmids, as well as the largest plasmid detectable in R. phaseoli...Full Text Available

1981-03-01

401

Isolation of Neisseria gonorrhoeae on selective and nonselective media in a sexually transmitted disease clinic.  

UK PubMed Central (United Kingdom)

To assess the practical significance of reported increases in the prevalence of vancomycin-susceptible strains of Neisseria gonorrhoeae on isolation of this organism, antibiotic-free chocolate agar...Full Text Available

1984-02-01

402

Intra- and Interspecies Conjugal Transfer of Tn916-Like Elements from Lactococcus lactis In Vitro and In Vivo?  

UK PubMed Central (United Kingdom)

Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using...Full Text Available

2009-10-01

403

Integrity assessment of 37 element fuel bundle of TAPS 3 and 4 reactor under beyond design basis accident condition  

International Nuclear Information System (INIS)

Tarapur Atomic Power Station 3 and 4 is a 540 MWe Pressurized Heavy Water Reactor. It uses 37 - element natural Uranium dioxide (UO_2) fuel pellets encapsulated inside the cylindrical sheath and are welded to the end plate at each end. During an postulated accident in which part of the fuel bundle are exposed to very high temperature (no means of heat removal) and other are at lower temperature (coolant temperature) possibility of failure of end plate weld due of thermal stresses developed by these relative temperature cannot be ruled out. In this report an attempt is made to study behaviour of fuel bundle under different temperature loading. Modelling of 37 element fuel bundle was done in ANSYS FEM. System was analysed for various sets of temperature loading. The system was analysed for plasticity and creep as material nonlinearity. The total strain, creep strain and stress increase as the temperature increases in upper portion of fuel bundle ...

2005-12-01

404

Instructional control of an autonomic sexual response1  

UK PubMed Central (United Kingdom)

Four of seven human male subjects developed full penile erections when exposed to erotically stimulating motion pictures. Changes in penile size were detected by a mercury strain gauge transducer and...Full Text Available

1969-01-01

405

Influence of parasite strain on chemotherapy of murine infections with schistosomiasis mansoni  

UK PubMed Central (United Kingdom)

The effectiveness of chemotherapy in human schistosomiasis varies from one area to another, and limited data from experimentally infected animals suggest inherent differences in the susceptibility...Full Text Available

1971-01-01

406

Influence of murine leukemia proviral integrations on development of N-methyl-N-nitrosourea-induced thymic lymphomas in AKR mice.  

UK PubMed Central (United Kingdom)

The AKR mouse strain is characterized by a high incidence of spontaneous thymic lymphoma that appears in older animals (greater than 6 months of age) and is associated with novel provirus integrations...Full Text Available

1991-11-01

407

In vivo cartilage contact strains in patients with lateral ankle instability  

UK PubMed Central (United Kingdom)

Damage to the anterior talofibular ligament (ATFL) and cacaneofibular ligament (CFL) during ankle sprain may be linked to the development of osteoarthritis. Altered tibiotalar kinematics have...Full Text Available

2010-09-17

408

Impact of Lifelong Sedentary Behavior on Mitochondrial Function of Mice Skeletal Muscle  

UK PubMed Central (United Kingdom)

This study investigated the impact of lifelong sedentariness on skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y;...Full Text Available

2009-09-01

409

Identification of the Haemophilus influenzae tolC Gene by Susceptibility Profiles of Insertionally Inactivated Efflux Pump Mutants  

UK PubMed Central (United Kingdom)

Isogenic strains containing insertional disruptions of 10 Haemophilus influenzae Rd genes were investigated for their effects on the susceptibility of the organism to various classes...Full Text Available

2004-04-01

410

Identification and mobilization by cointegrate formation of a nodulation plasmid in Rhizobium trifolii.  

UK PubMed Central (United Kingdom)

A nodulation plasmid, pRtr-514a, of molecular size 180 megadaltons (Mdal) was identified in Rhizobium trifolii strain NZP514. This plasmid was absent in both spontaneous and heat-cured Nod- derivatives...Full Text Available

1982-07-01

411

Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.  

UK PubMed Central (United Kingdom)

Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium...Full Text Available

1990-03-01

412

High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal  

British Library Electronic Table of Contents (United Kingdom)

Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50?g/L in 72?h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol prod...

2011-01-01

413

High-Throughput Screen for Poly-3-Hydroxybutyrate in Escherichia coli and Synechocystis sp. Strain PCC6803  

UK PubMed Central (United Kingdom)

A novel, quantitative method for detecting poly-3-hydroxybutyrate (PHB) amounts in viable cells was developed to allow for high-throughput screening of mutant libraries. The staining technique was demonstrated...Full Text Available

2006-05-01

414

Hemodynamics and Axial Strain Additively Increase Matrix Remodeling and MMP-9, But Not MMP-2, Expression in Arteries Engineered by Directed Remodeling  

UK PubMed Central (United Kingdom)

We previously demonstrated the ability to create engineered arteries by carefully controlling the mechanical environment of intact arteries perfused ex vivo, yielding engineered arteries...Full Text Available

2009-06-01

415

Growth temperature reversibly modulates the virulence of Legionella pneumophila.  

UK PubMed Central (United Kingdom)

In chemostat culture, the virulence of two strains of Legionella pneumophila was shown to be significantly (P < 0.05) reduced when the culture temperature was lowered from 37 to 24 degrees C....Full Text Available

1994-07-01

416

Growth of Staphylococcus aureus MF 31 on the Top and Cut Surfaces of Southern Custard Pies  

UK PubMed Central (United Kingdom)

A Staphylococcus strain was inoculated on the top and cut surfaces of freshly baked Southern custard pies which were then packaged in a pasteboard carton and held at 30 C. Daily plate...Full Text Available

1969-07-01

417

Gingival crevicular fluid levels of clindamycin compared with its minimal inhibitory concentrations for periodontal bacteria.  

UK PubMed Central (United Kingdom)

Clindamycin concentrations in gingival crevicular fluid and in blood were determined over a 7-h period and were related to the minimal inhibitory concentrations of this agent for 340 bacterial strains...Full Text Available

1981-05-01

418

Genetic analysis of the psychostimulant effects of nicotine in chromosome substitution strains and F2 crosses derived from A/J and C57BL/6J progenitors  

British Library Electronic Table of Contents (United Kingdom)

Previous research utilizing the AcB/BcA recombinant congenic strains (RCS) of mice mapped provisional quantitative trait loci (QTLs) for the psychostimulant effects of nicotine to multiple regions on chromosomes 7, 11, 12, 14, 16, and 17. The current study was designed to confirm these QTLs in an A/J (A)??C57Bl/6J (B6) F2 cross and a panel of B6.A chromosome substitution strains (CSS). The panel of B6.A CSS consists of 21 strains, each carrying a different A/J chromosome on a B6 background. The A??B6 F2, CSS, A, and B6 mice were tested for sensitivity to the effects of nicotine on locomotor activity using a computerized open-field apparatus. In A??B6 F2 mice two QTLs were identified which confirm those previously observed in the AcB/BcA RCS. Significant differences in the expression of ...

2009-01-01

419

Gallium Disrupts Iron Uptake by Intracellular and Extracellular Francisella Strains and Exhibits Therapeutic Efficacy in a Murine Pulmonary Infection Model ?  

UK PubMed Central (United Kingdom)

Francisella tularensis requires iron (Fe) for growth, but the biologic sources of Fe for this organism are largely unknown. We found that Francisella sp. growing in...Full Text Available

2010-01-01

420

Fermentability of Corn Syrups with Different Dextrose Equivalents Added to Various Grape Juices1  

UK PubMed Central (United Kingdom)

It was found that neither the enzymes of the grapes nor those of wine yeast Saccharomyces ellipsoideus strain 223 attacked the higher polysaccharides present in corn syrups. The alcohol...Full Text Available

1967-03-01

421

Factors affecting the aggregation of Actinomyces naeslundii during growth and in washed cell suspensions.  

UK PubMed Central (United Kingdom)

Various factors affecting the aggregation of Actinomyces naeslundii strain 12104 were studied. When the pH of glucose-supplemented growth medium fell below 5.5, the cells aggregated and formed microbial...Full Text Available

1978-09-01

422

Excitonic transitions in InGaP/InAlGaP strained quantum wells  

International Nuclear Information System (INIS)

Excitonic transitions in metalorganic vapor phase epitaxially grown In_xGa_1_-_xP/In_0_._4_8(Al_0_._7Ga_0_._3)_0_._5_2P strained single quantum-well structures are characterized using low-temperature photoluminescence and photoluminescence excitation (PLE) spectroscopies. The structures consist of several uncoupled quantum wells with thicknesses between 1.2 and 11.3 nm, and compositions x of 0.48 (nominally lattice matched) and 0.56 (#approx#0.6% biaxial compressive strain). The photoluminescence spectra exhibit intense peaks over the wavelength range 550--650 nm, with linewidths between 7 and 23 meV depending on the well thickness. The PLE spectra reveal strong heavy-hole and light-hole transitions, as well as higher-order (n=2) transitions in the thicker wells. The heavy-hole/light-hole splitting shows little dependence on well thickness in the strained structures, indicating a relatively large conduction band offset of ...

423

Epidemiological consequences of an incursion of highly pathogenic H5N1 avian influenza into the British poultry flock  

UK PubMed Central (United Kingdom)

Highly pathogenic avian influenza and in particular the H5N1 strain has resulted in the culling of millions of birds and continues to pose a threat to poultry industries worldwide. The recent outbreak...Full Text Available

2008-01-07

424

Emergence of Klebsiella pneumoniae Coproducing KPC-2 and 16S rRNA Methylase ArmA in Poland ?  

UK PubMed Central (United Kingdom)

A Klebsiella pneumoniae epidemic strain that coproduced carbapenemase KPC-2 (K. pneumoniae carbapenemase 2) and 16S rRNA methylase ArmA has emerged in Poland. Four...Full Text Available

2011-01-01

425

Elasto-plastic analysis of settlement of pile foundations. Part 2. ; Prediction of immediate settlement of building. Kui kiso no dansosei chinka kaiseki. 2. ; Kenchikubutsu no sokuji chinka yosoku hoho  

Energy Technology Data Exchange (ETDEWEB)

When the foundations of the building are designed and worked, the prediction calculation of immediate settlement of the building exercised practically is to calculate assuming the earth is a linear elastic body, but the actual stress-strain relationship of the earth is nonlinear and rigidity of the earth becomes lower as strain (or stress) increases. Hence in order to grasp the actual settling behavior of the earth, it is necessary to elucidate the nonlinear relationship between stress and strain. In this article, a prediction method of building settlement is introduced which uses an analytical technique of the multi-layered earth by the linear elasticity theory and takes into consideration strain dependency of rigidity of the earth. Part of the conclusion is as follows which has been obtained by studying the settling behaviors observed in examples of vertical loading tests of the pile foundations and ...

1992-12-25

426

Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine.  

UK PubMed Central (United Kingdom)

Phagocytosis and intracellular killing of two strains of Escherichia coli and a Staphylococcus saprophyticus by polymorphonuclear neutrophils (PMN) in pooled sterile urine at three osmolalities (800,...Full Text Available

1993-01-01

427

Effect of dietary green tea extract and aerosolized difluoromethylornithine during lung tumor progression in A/J strain mice  

UK PubMed Central (United Kingdom)

Chemoprevention strategies to prevent the development of lung cancer in at-risk individuals are a key component in disease management. In addition to being highly effective, an ideal chemopreventive...Full Text Available

2008-08-01

428

Dynamic nucleation of new grains in magnesium alloy during hot deformation  

Energy Technology Data Exchange (ETDEWEB)

Microstructure evolution under hot deformation was investigated in compression of a magnesium alloy AZ31 at 673 K (0.73T{sub m}). Two kinds of samples were machined along the parallel and transverse direction of the extruded rods. New fine grains are evolved at corrugated grain boundaries at low strains and developed rapidly in grain interiors in the medium range of strain, finally leading to a roughly full evolution of equiaxial fine grains. Kink bands are evolved at corrugated grain boundaries and in grain interiors at low strains. The boundary misorientation of kink band increases rapidly with increasing strain. These characteristics of new grain evolution process are not changed by the orientation of the samples, while the flow behaviors clearly depend on it. It is concluded that new grain evolution can be controlled by a deformation-induced continuous reaction resulting in grain fragmentation by ...

2003-07-01

429

Drug Susceptibility Testing of Mycobacterium tuberculosis by a Nitrate Reductase Assay Applied Directly on Microscopy-Positive Sputum Samples  

UK PubMed Central (United Kingdom)

Current methods for drug susceptibility testing of Mycobacterium tuberculosis are either costly or slow. As the prevalence of multidrug-resistant strains increases, the need for fast,...Full Text Available

2005-07-01

430

Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice  

UK PubMed Central (United Kingdom)

Uncoupling protein 2 (UCP2) maps to a region on distal mouse chromosome 7 that has been linked to the phenotypes of obesity and type II diabetes. We recently reported that UCP2 expression is increased...Full Text Available

1998-03-31

431

Development of a New Serological Test for Serotyping Haemophilus parasuis Isolates and Determination of Their Prevalence in North America  

UK PubMed Central (United Kingdom)

Haemophilus parasuis causes polyserositis in swine. Fifteen serovars have been characterized by immunodiffusion test, but many field strains are not typeable. Isolates (n...Full Text Available

2004-02-01

432

Correlates to Increased Lethality of Attenuated Venezuelan Encephalitis Virus Vaccine for Immunosuppressed Hamsters.  

Science.gov (United States)

Splenectomy or pretreatment of adult hamsters with cyclophosphamide (Cytoxan) increased the lethality of the TC-83 vaccine strain of Venezuelan encephalitis virus (VEE), inoculated subcutaneously, from 12% for normal hamsters to 75 and 76% respectively. A...

1974-01-01

433

Complete tree utilization of a fast growing species - eucalyptus hybrid  

Energy Technology Data Exchange (ETDEWEB)

Potential uses of foliage and bark wastes of E. ('Mysore') hybrid in southern India for the production of cineole and oxalic acid respectively are discussed. Only certain strains are cineole-rich and these are being successfully propagated vegetatively at the authors' laboratory.

1981-01-01

434

Complete Sequencing of pNDM-HK Encoding NDM-1 Carbapenemase from a Multidrug-Resistant Escherichia coli Strain Isolated in Hong Kong  

UK PubMed Central (United Kingdom)

BackgroundThe emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to...Full Text Available

435

Comparison of secondary metabolite production by Penicillium crustosum strains, isolated from Arctic and other various ecological niches.  

Science.gov (United States)

Penicillium crustosum is common in food and feed both in subtropical and temperate regions. Recently, it has also been found occurring frequently in glacier ice, sea ice and sea water of Arctic regions of Svalbard. The aim of the study was to compare isolates of the same fungal species from widely different habitats and geographic regions to see if the nutritional physiology and the profile of secondary metabolites were consistent or depended on the isolation source. All 121 strains examined produced the following families of secondary metabolites: penitrems (100%), roquefortines (100%), terrestric acids (99.2%) and viridicatols (100%), whereas 81 of 83 Arctic isolates additionally produced andrastin A. However, only 8 of 38 non-Arctic isolates produced detectable andrastin A. The quantitative profiles of 96 strains were compared using cluster, principal component and correspondence analyses. There was no clear grouping of Arctic versus ...

2004-12-22

436

Comparison of Genotypes of Salmonella enterica Serovar Enteritidis Phage Type 30 and 9c Strains Isolated during Three Outbreaks Associated with Raw Almonds?  

UK PubMed Central (United Kingdom)

In 2000 to 2001, 2003 to 2004, and 2005 to 2006, three outbreaks of Salmonella enterica serovar Enteritidis were linked with the consumption of raw almonds. The S....Full Text Available

2010-06-01

437

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.  

UK PubMed Central (United Kingdom)

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

438

Cloning and Characterization of lin Genes Responsible for the Degradation of Hexachlorocyclohexane Isomers by Sphingomonas paucimobilis Strain B90  

UK PubMed Central (United Kingdom)

Hexachlorocyclohexane (HCH) has been used extensively against agricultural pests and in public health programs for the control of mosquitoes. Commercial formulations of HCH consist of a mixture of four...Full Text Available

2002-12-01

439

Citrate Uptake in Exchange with Intermediates in the Citrate Metabolic Pathway in Lactococcus lactis IL1403?  

UK PubMed Central (United Kingdom)

Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while...Full Text Available

2011-02-01

440

Characterization of a Chromosomally Encoded Extended-Spectrum Class A ?-Lactamase from Kluyvera cryocrescens  

UK PubMed Central (United Kingdom)

A chromosomally located β-lactamase gene, cloned and expressed in Escherichia coli from a reference strain of the enterobacterial species Kluyvera cryocrescens,...Full Text Available

2001-12-01

441

Characterization of Thermoanaerobacter Glucose Isomerase in Relation to Saccharidase Synthesis and Development of Single-Step Processes for Sweetener Production  

UK PubMed Central (United Kingdom)

Regulation of glucose isomerase synthesis was studied in Thermoanaerobacter strain B6A, which fermented a wide variety of carbohydrates including glucose, xylose, lactose, starch, and...Full Text Available

1990-09-01

442

Changes in colonic motility induced by sennosides in dogs: evidence of a prostaglandin mediation.  

UK PubMed Central (United Kingdom)

The effects of sennosides on colonic motility were investigated in eight conscious dogs chronically fitted with two strain gauge transducers in the proximal colon, an intracolonic silicone catheter...Full Text Available

1988-09-01

443

Cement-based composites: Strain rate effects on fracture  

Energy Technology Data Exchange (ETDEWEB)

This book contains over 20 selections. Some of the titles are: Continuum damage mechanics studies on the dynamic fracture of concrete; Dynamic compressive strength of cementitious materials; Rate-sensitivity of mode I and mode II fracture concrete; and An impact damage model of concrete.

1986-01-01

444

Capacity of staphylococci to grow in the presence of ovotransferrin or CrCl3 as a character of potential pathogenicity.  

UK PubMed Central (United Kingdom)

A total of 150 strains of staphylococci, clinically isolated, were tested for sensitivity to ovotransferrin (conalbumin). Among these, all the 50 coagulase-positive, mannitol-positive, and deoxyribonuclease-positive...Full Text Available

1980-05-01

445

CTX-M ?-Lactamase Production and Virulence of Escherichia coli K1  

UK PubMed Central (United Kingdom)

We report a patient with neonatal meningitis caused by a CTX-M-1–producing Escherichia coli K1 strain. The influence of CTX-M production on virulence was investigated in cell...Full Text Available

2009-12-01

446

CD44 as a receptor for colonization of the pharynx by group A Streptococcus  

UK PubMed Central (United Kingdom)

The pharynx is the primary reservoir for strains of group A Streptococcus (GAS) associated both with pharyngitis (streptococcal sore throat) and with invasive or “flesh-eating”...Full Text Available

2000-10-15

447

Biological processing and interactions with coal: Summary of activities for the period January 1, 1988--March 31, 1988  

Science.gov (United States)

Biodegradation/solubilization of coal is described. The degradation of dibenzothiophene by two bacterial strains is reported. The effects of adding salicylate to the treatments was also investigated. 2 figs., 5 tabs. (CBS)

1988-01-01

448

Bacterial competition between a bacteriocin-producing and a bacteriocin-negative strain of Streptococcus bovis in batch and continuous culture:  

British Library Electronic Table of Contents (United Kingdom)

Abstract A bacteriocin-producing Streptococcus bovis strain (HC5) outcompeted a sensitive strain (JB1) before it reached stationary phase (pH 6.4), even though it grew 10% slower and cell-free bovicin HC5 could not yet be detected. The success of bacteriocin-negative S. bovis isolates was enhanced by the presence of another sensitive bacterium (Clostridium sticklandii SR). PCR based on repetitive DNA sequences indicated that S. bovis HC5 was not simply transferring bacteriocin genes to S. bovis JB1. When the two S. bovis strains were coinoculated into minimal medium, bacteriocin-negative isolates predominated, and this effect could be explained by the longer lag time (0.5 vs. 1.5 h) of S. bovis HC5. If the glucose concentration of the minimal medium was increased from 2 to 7 mg mL-1, the e...

2006-01-01

449

Assay for the enzymatic conversion of indoleacetic acid to 3-methylindole in a ruminal Lactobacillus species.  

UK PubMed Central (United Kingdom)

An assay to measure the rate of enzymatic formation of 3-methylindole (3MI) from indoleacetic acid (IAA) in Lactobacillus sp. strain 11201 was developed. The reaction mixture contained 50 micrograms...Full Text Available

1990-03-01

450

Antimicrobial susceptibilities of bacteria associated with periodontal disease.  

UK PubMed Central (United Kingdom)

A total of 193 bacterial strains were tested for their susceptibilities to 14 antimicrobial agents. Penicillin G was active at 2 U/ml against 98% of the oral isolates. Other antibiotics with good activity...Full Text Available

1983-03-01

451

Antidepressant-Like Effects of ?-Opioid Receptor Antagonists in Wistar Kyoto Rats  

UK PubMed Central (United Kingdom)

The Wistar Kyoto (WKY) rat strain is a putative genetic model of comorbid depression and anxiety. Previous research showing increased κ-opioid receptor (KOR)...Full Text Available

2010-02-01

452

Antibacterial Colorants: Characterization of Prodiginines and Their Applications on Textile Materials  

UK PubMed Central (United Kingdom)

A strain of Vibrio sp. isolated from marine sediments produced large quantities of bright red pigments that could be used to dye many fibers including wool, nylon, acrylics,...Full Text Available

2008-05-01

453

Analysis of the genome-wide variations among multiple strains of the plant pathogenic bacterium Xylella fastidiosa  

UK PubMed Central (United Kingdom)

BackgroundThe Gram-negative, xylem-limited phytopathogenic bacterium Xylella fastidiosa is responsible for causing economically important diseases in grapevine,...Full Text Available

454

Analysis of Haemophilus parasuis isolates from southern Ontario swine by restriction endonuclease fingerprinting.  

UK PubMed Central (United Kingdom)

To study the occurrence and distribution of various strains of Haemophilus parasuis in southern Ontario swine, organisms isolated from healthy swine, from specific pathogen-free and conventional herds,...Full Text Available

1988-07-01

455

An investigation of enzootic Glasser's disease in a specific-pathogen-free grower-finisher facility using restriction endonuclease analysis  

UK PubMed Central (United Kingdom)

Enzootic Glassers's disease was investigated to study the epidemiology of the disease strains on a farm where it presented a problem. Restriction endonuclease fingerprinting (REF) analysis technique...Full Text Available

1993-08-01

456

A survey of the sensitivity of fresh clinical isolates to cefuroxime and other antibiotics.  

UK PubMed Central (United Kingdom)

The sensitivities to cefuroxime and cephradine of potentially pathogenic bacteria isolated in two British general hospitals comprising 900 beds have been assessed. In a three-month period 2537 strains...Full Text Available

1979-09-01

457

A study of the genetics of dieldrin-resistance in the housefly (Musca domestica L.)*  

UK PubMed Central (United Kingdom)

Reciprocal mass crosses and back-crosses were performed between two homogeneous strains of the housefly (Musca domestica L.), representing the extremes in susceptibility and resistance...Full Text Available

1963-01-01

458

A role of ygfZ in the Escherichia coli response to plumbagin challenge  

UK PubMed Central (United Kingdom)

Plumbagin is found in many herbal plants and inhibits the growth of various bacteria. Escherichia coli strains are relatively resistant to this drug. The mechanism of resistance is...Full Text Available

459

A novel rat strain with enhanced sensitivity to the effects of dopamine agonists on startle gating  

UK PubMed Central (United Kingdom)

BackgroundCompared to outbred Sprague Dawley (SD) rats, inbred Brown Norway (BN) rats exhibit less prepulse inhibition of startle (PPI) at long prepulse intervals,...Full Text Available

2008-01-01

460

A new evolutionary scenario for the Mycobacterium tuberculosis complex  

UK PubMed Central (United Kingdom)

The distribution of 20 variable regions resulting from insertion-deletion events in the genomes of the tubercle bacilli has been evaluated in a total of 100 strains of Mycobacterium tuberculosis,...Full Text Available

2002-03-19

461

A mechanistic understanding of the formability of magnesium: examining the role of temperature on the deformation mechanisms  

Energy Technology Data Exchange (ETDEWEB)

The possibility of improving the cold formability of wrought magnesium alloys is considered in light of their good hot forming characteristics. Magnesium alloy AZ31B sheet is selected as a model system. Parameters affecting formability, such as strain hardening rate, strain rate sensitivity, and the degree of anisotropy are examined systematically by conducting tensile tests over a range of temperatures (room temperature to 250 C) and strain rates (1 x 10{sup -5} to 0.1 s{sup -1}). The plastic anisotropy and deformation texture evolution are examined in samples aligned with the sheet rolling and transverse directions. Polycrystal plasticity simulations using a viscoplastic self-consistent (VPSC) formulation are used to model the observed anisotropy and texture evolution. The adjustable parameters in the model are the relative critical resolved shear stresses of the dislocation mechanisms known to operate within magnesium. ...

2003-07-01

462

Tumorigenic and tumoricidal actions of ionizing radiations  

Energy Technology Data Exchange (ETDEWEB)

The book is divided into two approximately equal parts. The first four chapters are relatively lengthy and cover the basic principles of radiation biology, carcinogenesis and therapy, along with a brief introduction to radiological physics to orient the reader without background in this specialized related discipline. The remainder consists of twenty-four relatively brief chapters, each covering the radiation biology of a specific organ, tissue, or systems tissues, with emphasis on the tumorigenic and tumoricidal action of ionizing radiations.

1983-01-01

463

MR imaging of musculoskeletal trauma to the pelvis and the lower limb  

Energy Technology Data Exchange (ETDEWEB)

Magnetic resonance imaging can be used in the trauma setting to detect fractures and associated soft tissue injuries of the musculoskeletal system. Magnetic resonance imaging complements plain radiography and CT for evaluating cancellous bone, cartilage and growth-plate injuries, and intra- and extraarticular supporting soft tissue structures. This review outlines typical applications and imaging consideration for injuries of the pelvis and lower extremity. (orig.) With 12 figs., 78 refs.

1999-03-01

464

Limiting-dilution analysis of T cells extracted from solid human lung tissue: comparison of precursor frequencies for proliferative responses and lymphokine production between lung and blood T cells from individual donors.  

UK PubMed Central (United Kingdom)

This study evaluates the frequency and functions of immunocompetent T cells at the clonal level in solid human lung tissue versus peripheral blood. Enzymatic digestion of slices of histologically normal...Full Text Available

1988-08-01

465

Inertinite of fossil coal and molecular paleontology  

British Library Electronic Table of Contents (United Kingdom)

The etching method developed made it possible to observe under the microscope the finest components of the anatomical structure of coal-forming plants. The dependence of the effectiveness of etching of tissues on the concentrations of geopolymers (protocellulose and protolignin) in them was found. The study of tissues of this kind by the methods of local analysis (for example, X-ray microanalysis) allowed us to obtain the characteristics of the above geopolymers and to use them for the identification of the primary biochemical composition of fossil organic matter.

2011-01-01

466

Immunohistochemical localization of low density lipoprotein receptor-related protein 1 and ?2-Macroglobulin in retinal and choroidal tissue of proliferative retinopathies  

UK PubMed Central (United Kingdom)

The immunolocalization of the low density lipoprotein receptor-related protein 1 (LRP1) and its ligand alpha 2-Macroglobulin (α2M) was examined in tissues from human donor...Full Text Available

2010-08-01

467

Ethylene-Enhanced Catabolism of [14C]Indole-3-Acetic Acid to Indole-3-Carboxylic Acid in Citrus Leaf Tissues 1  

UK PubMed Central (United Kingdom)

Exogenous [14C]indole-3-acetic acid (IAA) is conjugated in citrus (Citrus sinensis) leaf tissues to one major substance which has been identified as indole-3-acetylaspartic...Full Text Available

1990-01-01

468

Electrical properties of retinal electrode interface  

Science.gov (United States)

A critical element of a retinal prosthesis is the stimulating electrode array, which is placed in close proximity to the retina. It is via this retinal-electrode interface that a retinal prosthesis electrically stimulates nerve cells to produce the perception of light. The impedance load seen by the current driver consists of the tissue resistance and the complex electrode impedance. The results in this paper show that the tissue resistance of the retina is significantly greater than that of the vitreous humor in the eye. Circuit models of the electrode-retina interface are used to parameterize the different contributors to the overall impedance.

2007-03-01

469

Comparative tissue distribution and excretion of orally administered ["3H]diacetoxyscirpenol (anguidine) in rats and mice  

International Nuclear Information System (INIS)

A quantitative comparison of tissue distribution and excretion of an orally administered sublethal dose of [3H]diacetoxyscirpenol (anguidine) was made in rats and mice 90 min, 24 hr, and 7 days after treatment. Total recoveries of 95-100% were obtained. Approximately 90% of the dose was excreted in urine and feces during the first 24 hr with a feces:urine ratio of about 1:4.5 in both species. Carcass and tissue radioactivity dropped rapidly during the first 24 hr but remained relatively constant at low, but detectable, levels over the course of the experiment. Few substantive interspecies differences were noted in tissue distribution. At 90 min the highest percentage of dose was in tissues involved in sequestering diacetoxyscirpenol because of high body water/lipid content or the absorption, metabolism, or excretion of the toxin. The rank order of these tissues was generally stable ...

470

Biochemical changes in human cervical connective tissue after local application of prostaglandin E2  

DEFF Research Database (Denmark)

Intracervical application of prostaglandin E2 in the late first trimester induces (1) softening of the cervix tissue; (2) increase in sulfated glycosaminoglycans (18 +/- 12%, mean +/- SEM); (3) no change in hyaluronic acid and water; (4) decrease in pepsin-extractable collagen, and (5) apparent decrease in collagenase. A high activity of collagenase in combination with a replacement of collagen with sulfated glycosaminoglycans may be of importance for the ripening process.

1983-01-01

471

A detailed comparison of the 5'-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA.  

UK PubMed Central (United Kingdom)

We have examined homologous fragments of DNA cloned from two different tissues for changes in the dNA sequence which might be related to tissue specific gene expression. The 5' end of the chicken ovalbumin...Full Text Available

1980-10-10

472

Theoretical investigations of strength of a flexible tube made of NiTi; Festigkeitstheoretische Untersuchungen einer flexiblen Huelse aus NiTi  

Energy Technology Data Exchange (ETDEWEB)

A flexible, steerable tube (distal end) of an endoscope for the application in the laparoscopic surgery has been analyzed by means of the finite-element-method (FEM). The unusual mechanical and thermal features of the used shape memory alloy (SMA) of NiTi were taken in account. The analyses showed that the strains remain in the super-elastic region up to the maximum bending of 90 and no plastic deformations occur. The application of the shape optimization (CAO) made possible finding the optimal structure of the distal end and therefore powerful reducing the maximum strains in the critical regions. (orig.)

2000-04-01

473

Study of deferred luminescence emitted by some species of chlorella after #gamma# irradiation at 77 K  

International Nuclear Information System (INIS)

The deferred luminescence (isothermal luminescence -ITL- and thermoluminescence - RTL) emitted by Chlorella cells after #gamma# irradiation at 77 K has been investigated. The decay kinetics of ITL as well as the effect of illumination of both ITL and RTL show that trapped electrons take part in the recombination reactions which give rise to both emissions, and that ITL can be attributed to electron tunnelling. Most strains of Chlorella studied present similar RTL glow curves, the only exceptions found being Chlorella vulgaris (green and bleached strains).

474

Structural integrity of whipping pipes following a postulated circumferential break - a contribution to determining strain levels acceptable under faulted conditions  

Energy Technology Data Exchange (ETDEWEB)

It is postulated that a break of a thin-walled pipe does not cause a subsequent break in the pipe in the vicinity of a plastic hinge even when the wall is weakened by a 60 circumferential crack of a depth of 30% of the wall thickness on the tension side. This pipe behavior is the result of plastic buckling in the compression side and applies to pipes of diameter-to-thickness ratio larger than 20. For this type of pipe, the axial strains decrease with increasing diameter-to-thickness ratio in the tension side. As the pipe is only loaded in one direction, there is no cyclic behavior that can trigger a subsequent break. (orig.)

1993-10-01

475

Stability of coherently strained semiconductor superlattices  

International Nuclear Information System (INIS)

The excess energy of several III-V and II-VI strained-layer semiconductor superlattices (AC)_p(BC)_p is studied as a function of the repeat period p and orientation G=[001], [110], [111], and [201], using first-principles calculations. We discover a number of universal features, including the predicted instability for nearly all p's and G's with respect to bulk disproportionation, the identification of chalcopyrite as a metastable ordered structure, and the stability of all thin epitaxial [110] and [201] and most common-anion [001] superlattices relative to coherent phase separation.

476

Simulation of self-assembled nanopatterns in strained 2D alloys on the face centered cubic (111) surface  

Energy Technology Data Exchange (ETDEWEB)

We investigate the formation of nanostructures in 2D strained alloys on face centered cubic (111) surfaces by means of equilibrium Monte Carlo simulations. In the framework of an off-lattice model, we consider one monolayer of two bulk-immiscible adsorbates A and B with negative and positive misfit relative to the substrate, respectively. Simulations show that the adsorbates partly self-organize into island or stripe-like patterns. We show how these structures depend on the relative misfits, interaction, and concentration of components. The morphology is quite different for phase separation and intermixing regimes.

2008-07-02

477

Observation of stochastic resonance near a subcritical bifurcation  

Energy Technology Data Exchange (ETDEWEB)

A hysteretic subcritical period-doubling bifurcation is observed in the nonlinear strain dynamics of a magnetostrictive oscillator. The dynamic strain response of the magnetostrictive oscillator was observed with a high-resolution fiber optic interferometer. The effects of low-frequency modulation and band-limited stochastic fluctuations on such a bifurcation are investigated. Power spectral density measurements show that for an optimal value of externally injected noise the signal-to-noise ratio of a low-frequency modulation signal is enhanced by greater than 14 dB, thus indicating the first experimental observation of stochastic resonance near a bistable period-doubling bifurcation. 10 refs., 7 figs.

1993-01-01

478

Effect of intense plastic straining on microstructure and mechanical properties of an Al-Mg-Sc alloy  

International Nuclear Information System (INIS)

An Al-5%Mg-0.18%Mn-0.2%Sc-0.08%Zr-0.002%Be was subjected to equal-channel angular extrusion up to true strains of #approx#3 and #approx#8, that resulted in the formation of partially recrystallized and fully recrystallized structure, respectively. It was shown that the alloy with partially recrystallized structure exhibits highest strength and ductility. The material with fully recrystallized structure showed lowest fatigue crack growth rate and highest value of fracture toughness. Reasons of this unusual effect of microstructure on crack propagation resistance under fatigue are discussed.

2010-07-01

479

Creation of a Cellooligosaccharide-Assimilating Escherichia coli Strain by Displaying Active Beta-Glucosidase on the Cell Surface via a Novel Anchor Protein.  

Science.gov (United States)

We demonstrated direct assimilation of cellooligosaccharide using Escherichia coli displaying beta-glucosidase (BGL). BGL from Thermobifida fusca YX (Tfu0937) was displayed on the E. coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h. PMID:21742905

2011-07-01

480

Trace Elements in Human Myocardial Infarction Determined by Neutron Activation Analysis  

International Nuclear Information System (INIS)

By means of neutron activation analysis, injured and adjacent uninjured human heart tissue from 12 autopsy cases with myocardial infarction are investigated with respect to the concentration of 23 trace elements. The bulk elements K, Na and P are also determined. A recently developed ion-exchange technique, combined with subsequent y-spectrometry, is used. The following trace elements are determined: Ag, As, Au, Ba, Br, Ca, Cd, Ce, Co, Cr, Cs, Cu, Fe, Hg, La, Mo, Rb, Sb, Sc, Se, Sm, Zn and W. In the injured tissue compared to the uninjured, calculation on a wet weight basis showed a decrease in Co, Cs, K, Mo, P, Rb and Zn, and an increase in Br, Ca, Ce, La, Na, Sb and Sm. The differences in Ca, La, Mo, P and Zn are dependent on the age of the myocardial infarction, and the regression lines for these elements are given. The concentration of the trace elements in uninjured tissue from infarcted hearts is compared to the ...

1965-01-01

481

The role of computed tomography in complex facial trauma  

Energy Technology Data Exchange (ETDEWEB)

Tridimensional localization of bone and soft tissue injuries in complex facial trauma is fundamental for treatment. The face can be conceptualized as 3 groups of interconnected osseous struts that are oriented in the horizontal, sagittal and coronal planes. Each group of struts is closely related to specific soft tissue structures that are susceptible to injury. Any single CT section in the coronal plane or in the axial plane contains parts of one or more these struts. Facial CT in biplane were performed in 32 patients of facial trauma. The most common site of facial fractures is found in middle part of horizontal struts, geometrically in anteromedial portion of the inferior orbital fissure of the face. The most common soft tissue injury combined with facial fracture is hematoma and/or herniated orbital contents into the maxillary sinus. The serious combined soft tissue injuries were ocular rupture, ...

1988-02-15

482

Radioisotopic techniques for noninvasive detection of platelet deposition in bovine-tissue mitral-valve prostheses and in vitro quantification of visceral microembolism in dogs  

International Nuclear Information System (INIS)

Platelet deposition on bovine pericardial-tissue mitral-valve prostheses in 11 dogs was observed noninvasively by use of "1"1"1In-labeled platelets and quantified after sacrifice at one (n . 3), 14 (n . 3), and 30 (n . 5) days postimplantation (300-400 microCi of labeled platelets having been injected 24 hours previously). Thrombosis on the sewing ring and pericardial leaflets at one and 14 days and on the leaflets at 30 days was delineated in scintiphotos. In vitro quantification (% injected dose) indicated that the leaflets, sewing ring, and perivalvular tissue retained 0.904% of labeled platelets at one day postimplantation, 0.198% at 14 days, and 0.040% at 30 days. Platelet half-life was reduced to 38 hours at 21 days postimplantation but returned toward the normal (50 hours) with fibrous ingrowth in the sewing ring. Microembolism in lung and kidney, as measured by tissue/blood radioactivity ratio, also was decreased ...

483

Phthalate monoesters in perfusate from a dual placenta perfusion system, the placenta tissue and umbilical cord blood.  

Science.gov (United States)

Fetal exposure to phthalates may be associated with adverse reproductive effects, including cryptorchidism and decreased semen quality. Information about human placental transfer is needed to qualify the hypotheses. A dual recirculating placenta perfusion system to monitor concentrations of eight phthalate monoesters in fetal and maternal perfusates was established. In addition to perfusate background measures of phthalate monoesters, the concentrations in umbilical cord plasma and placenta tissue were measured. Monomethyl phthalate (mMP), monoethyl phthalate (mEP), monobutyl phthalate (mBP), and mono (2-ethyl-hexyl) phthalate (mEHP) were detected in both maternal and fetal perfusate, demonstrating a release of compounds from tissue or blood to perfusates. The distribution of compounds between perfusate, umbilical cord plasma, and tissue was in accordance with the physical-chemical properties of the compounds. Results from ...

2006-09-01

484

Historical overview of wound ballistics research.  

Science.gov (United States)

Ballistics involves the study of the scientific properties of projectiles, their behavior and their terminal effects on biological tissues and other materials. Wound ballistics deals with the analysis of injuries caused by projectiles and the behavior of projectiles within human or other biological tissues. The nineteenth century witnessed the development of both of these areas with Kocher's hydrodynamic theory and the understanding of the significance of bullet deformation in causing tissue injury. The degree of traumatic disruption of tissues and organs was also related to direct energy transfer from projectiles. While subsequent research has concentrated on elucidating further mechanisms of injury, the exact cause of remote tissue damage from high energy projectiles is still the subject of ongoing research. Much of the contemporary literature regarding wound ballistics ...

2009-05-23

485

Challenges in validating the sterilisation dose for processed human amniotic membranes  

Energy Technology Data Exchange (ETDEWEB)

Most of the tissue banks in the Asia Pacific region have been using ionising radiation at 25 kGy to sterilise human tissues for save clinical usage. Under tissue banking quality system, any dose employed for sterilisation has to be validated and the validation exercise has to be a part of quality document. Tissue grafts, unlike medical items, are not produced in large number per each processing batch and tissues relatively have a different microbial population. A Code of Practice established by the International Atomic Energy Agency (IAEA) in 2004 offers several validation methods using smaller number of samples compared to ISO 11137 (1995), which is meant for medical products. The methods emphasise on bioburden determination, followed by sterility test on samples after they were exposed to verification dose for attaining of sterility assurance level (SAL) of 10{sup -1}. This paper ...

2007-11-15

486

Biological wound dressings sterilized with gamma radiation: Mexican clinical experience  

International Nuclear Information System (INIS)

Biological wound dressings sterilized with gamma radiation, such as amnion and pig skin, are a reality in Mexico. These tissues are currently processed in the tissue bank and sterilized in the Gamma Industrial Irradiation Plant; both facilities belong to the Instituto Nacional de Investigaciones Nucleares (ININ) (National Institute of Nuclear Research). With the strong support of the International Atomic Energy Agency, the bank was established at the ININ and the Mexican Ministry of Health issued its sanitary license on July 7, 1999. The Quality Management System of the bank was certified by ISO 9001:2000 on August 1, 2003; the scope of the system is 'Research, Development and Processing of Biological Tissues Sterilized with Gamma Radiation'. At present, more than 150 patients from 16 hospitals have been successfully treated with these tissues. This paper presents a brief description of the ...

2007-11-01

487

The effect of intratracheal administration of a surfactant on mortality in a model of murine paraquat poisoning  

International Nuclear Information System (INIS)

''Paraquat lung'' which is complicated with paraquat poisoning has been a lethal pulmonary pathology presenting intra-alveolar fibrosis, but an effective therapy has not been developed so far. We hypothesized that the type II alveolar cells producing surfactant were damaged by paraquat which was actively accumulated through out the blood by alveolar epithelial cells. To prove this hypothesis, we examined the effect of an intratracheal administration of an artificial lung surfactant (surfacten, Mitsubishi Tanabe Pharma Corporation, Osaka) on mortality in a model of murine paraquat poisoning. Paraquat was given intramuscularly 3 days after the intratracheal surfactant administration. The mice used were C57BL/6J strain and Balb/C strain. The lethal dose, 50% (LD50), of paraquat was about 28 mg/kg in the C57BL/6J strain and about 9 mg/kg in the Balb/C strain, respectively. Mortalities of paraquat poisoning ...

488

Slow strain-rate testing of Alloy 800 in molten-nitrate salts  

Energy Technology Data Exchange (ETDEWEB)

An experimental technique has been developed to examine the interaction between deformation and the exposure of certain high temperature structural alloys to oxidizing molten salt environments. The experimental program involved performing a series of long-term tensile tests over a wide range of strain rates. Fracture strain reduction in area and ultimate strength (UTS) were monitored as parameters indicative of an alloy's susceptibility to environmental degradation. For Incoloy Alloy 800 tested at 600/sup 0/C in the salt medium and at initial strain rates between 2 x 10/sup -7/ sec/sup -1/ and 1 x 10/sup -5/ sec/sup -/1 no appreciable loss of ductility, as measured by reduction in area, was observed relative to control specimens tested in air at the same temperature and strain rates. Similarly, fracture strain and UTS were essentially unaffected by exposure to the oxidizing ...

1982-01-01

489

Identification of unusual Campylobacter-like isolates from poultry products as Helicobacter pullorum  

DEFF Research Database (Denmark)

Twenty-six unclassified Campylobacter-like strains previously isolated from 15 chicken carcasses and caecal contents, together with two more strains isolated from chicken faeces on a different occasion, were identified as Helicobacter pullorum using various phenotypic identification methods. API Campy identification kits and a 16-test identification scheme developed for campylobacters failed to identify these bacteria, or identified them as Campylobacter spp. Eighteen strains (including the two isolated on a different occasion) were chosen for examination using a more comprehensive probabilistic identification scheme. Using this method, 14 of the 18 strains were identified as H. pullorum with ID scores > 95%; two strains were also identified as H. pullorum with lower ID scores. Of the remaining two strains, one was not identified with this scheme and the ...

1998-01-01

490

A Flow Stress Formulation of Magnesium Alloy at Elevated Temperature  

International Nuclear Information System (INIS)

Dynamic recrystallization (DRX) is the main softening mechanism of magnesium alloy AZ31B in hot deformation. Theoretically, in the flow rule the atomic diffusibility and the driving force of dislocation migration are dependent on the temperature, and the dislocation density and the cumulation of grain boundary energy are dependent on the strain rate. The peak stress will appear when the flow driving force and resistance force reach a balance, after which the stress descending will take place due to recrystallization fraction. Since the DRX is a thermally activated process, the recrystallized volume fraction can be regarded as the function of strain through Avrami equation. Based on this idea, the paper proposes a new constitutive model characterizing dynamic recrystallization for magnesium alloy AZ31B. The model is described by a peak stress and a strain softening rate, in which the peak stress depends only on ...

2007-05-17

491

Time-resolved confocal fluorescence spectroscopy reveals the structure and metabolic state of epithelial tissue  

Science.gov (United States)

Autofluorescence spectroscopy has been a widely explored technique for in vivo and noninvasive diagnosis of pre-cancer lesions in epithelium where 90% cancers originate. For extracting more accurate fluorescence information for cancer diagnosis, depth-resolved fluorescence measurements are crucial to assess NADH and FAD in non-keratinized epithelial layer and collagen in stromal layer, respectively. In this study, we achieved the depth-resolved fluorescence spectral measurements of squamous epithelial tissue based on confocal technique. We found that in non-keratinized epithelial layer the fluorescence signals excited at 405 nm were the combination of NADH and FAD fluorescence and could be used for evaluating the redox ratio. Moreover, we found that confocal time-resolved autofluorescence measurements of epithelial tissue with 405 nm excitations could provide the information on the layered tissue structure. All ...

2007-03-01

492

Population doses from beam-therapy in Japan, 1978, 3  

International Nuclear Information System (INIS)

As a series of estimations of population doses and of risk estimates from medical exposures in Japan, the malignancy significant dose (MSD) and the fatal malignant risk from beam therapy were estimated based on a nationwide survey of radiotherapeutic treatments, using a malignancy significant factor and a weighting factor determined from the data on the cancer mortality among the atomic bomb survivors in Nagasaki. The effective dose was defined as a sum of the product of the weighting factor and the organ or tissue doses with respect to the malignant diseases. The organ or tissue doses were determined with ionization chambers placed at the positions of their center in a MixDp-phantom simulated lung tissues by a block of cork, using a telecobalt unit, a conventional X-ray unit and a medical linear accelerator. The organ or tissue doses were categorized into three dose components; namely 1) dose from ...

1981-01-01

493

Mercury and other trace elements in a pelagic Arctic marine food web (Northwater Polynya, Baffin Bay)  

Energy Technology Data Exchange (ETDEWEB)

Total mercury (THg), methylmercury (MeHg) and 22 other trace elements were measured in ice algae, three species of zooplankton, mixed zooplankton samples, Arctic cod (Boreogadus saida), ringed seals (Phoca hispida) and eight species of seabirds to examine the trophodynamics of these metals in an Arctic marine food web. All samples were collected in 1998 in the Northwater Polynya (NOW) located between Ellesmere Island and Greenland in Baffin Bay. THg and MeHg were found to biomagnify through the NOW food web, based on significant positive relationships between log THg and log MeHg concentrations vs. {delta} {sup 15}N muscle and liver . The slope of these relationships for muscle THg and MeHg concentrations (slope = 0.197 and 0.223, respectively) were similar to those reported for other aquatic food webs. The food web behavior of THg and {delta} {sup 15}N appears constant, regardless of trophic state (eutrophic vs. oligotrophic), latitude (Arctic vs. tropical) or salinity (marine vs. ...

2005-12-01

494

Mercury and other trace elements in a pelagic Arctic marine food web (Northwater Polynya, Baffin Bay)  

International Nuclear Information System (INIS)

Total mercury (THg), methylmercury (MeHg) and 22 other trace elements were measured in ice algae, three species of zooplankton, mixed zooplankton samples, Arctic cod (Boreogadus saida), ringed seals (Phoca hispida) and eight species of seabirds to examine the trophodynamics of these metals in an Arctic marine food web. All samples were collected in 1998 in the Northwater Polynya (NOW) located between Ellesmere Island and Greenland in Baffin Bay. THg and MeHg were found to biomagnify through the NOW food web, based on significant positive relationships between log THg and log MeHg concentrations vs. #delta# "1"5N muscle and liver . The slope of these relationships for muscle THg and MeHg concentrations (slope = 0.197 and 0.223, respectively) were similar to those reported for other aquatic food webs. The food web behavior of THg and #delta# "1"5N appears constant, regardless of trophic state (eutrophic vs. oligotrophic), latitude (Arctic vs. tropical) or salinity (marine vs. freshwater) ...

2005-12-01

495

Maximum imaging depth of two-photon autofluorescence microscopy in epithelial tissues  

Science.gov (United States)

Endogenous fluorescence provides morphological, spectral, and lifetime contrast that can indicate disease states in tissues. Previous studies have demonstrated that two-photon autofluorescence microscopy (2PAM) can be used for noninvasive, three-dimensional imaging of epithelial tissues down to approximately 150 ?m beneath the skin surface. We report ex-vivo 2PAM images of epithelial tissue from a human tongue biopsy down to 370 ?m below the surface. At greater than 320 ?m deep, the fluorescence generated outside the focal volume degrades the image contrast to below one. We demonstrate that these imaging depths can be reached with 160 mW of laser power (2-nJ per pulse) from a conventional 80-MHz repetition rate ultrafast laser oscillator. To better understand the maximum imaging depths that we can achieve in epithelial tissues, we studied image contrast as a function of depth in ...

2011-02-01

496

Mass spectrometric characterization of elements and molecules in cell cultures and tissues  

Energy Technology Data Exchange (ETDEWEB)

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and laser post-ionization secondary neutral mass spectrometry (laser-SNMS) have been used to image and quantify targeted compounds, intrinsic elements and molecules with subcellular resolution in single cells of both cell cultures and tissues. Special preparation procedures for analyzing cell cultures and tissue materials were developed. Cancer cells type MeWo, incubated with boronated compounds, were sandwiched between two substrates, cryofixed, freeze-fractured and freeze-dried. Also, after injection with boronated compounds, different types of mouse tissues were extracted, prepared on a special specimen carrier and plunged with high velocity into LN{sub 2}-cooled propane for cryofixation. After trimming, these tissue blocks were freeze-dried. The measurements of the K/Na ratio demonstrated that for both cell cultures and ...

2006-07-30

497

Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization  

British Library Electronic Table of Contents (United Kingdom)

Isolation of functional and intact mitochondria from solid tissue is crucial for studies that focus on the elucidation of normal mitochondrial physiology and/or mitochondrial dysfunction in conditions such as aging, diabetes, and cancer. There is growing recognition of the importance of mitochondria both as targets for drug development and as off-target mediators of drug side effects. Unfortunately, mitochondrial isolation from tissue is generally carried out using homogenizer-based methods that require extensive operator experience to obtain reproducible high-quality preparations. These methods limit dissemination, impede scale-up, and contribute to difficulties in reproducing experimental results over time and across laboratories. Here we describe semiautomated methods to disrupt tissue ...

2011-01-01

498

Evaluation of the Soft and Hard Tissue Changes After Anterior Segmental Osteotomy on the Maxilla and Mandible  

British Library Electronic Table of Contents (United Kingdom)

PurposeThe purpose of this study was to determine the relationship between the changes of soft and hard tissues after modified anterior segmental osteotomy on the maxilla and mandible and to evaluate unintended facial changes using cephalometric and photometric analyses.Materials and MethodsThe subjects included 29 women and 1 man (22 to 50 years of age) who were diagnosed as bialveolar or bimaxillary protrusion and underwent modified anterior segmental osteotomy on the maxilla and mandible. Lateral cephalograms and lateral and frontal photographs taken preoperatively and postoperatively were analyzed.ResultsThere was a significant change in all soft and hard tissue parameters except the labiomental angle. The ratio of upper lip to maxillary incisor retraction was 0.67:1 and the ratio of l...

2008-01-01

499

Comparison of the results obtained by CALUX bioassay and GC-HRMS for different matrices  

Energy Technology Data Exchange (ETDEWEB)

The reference method used to analyse polychlorodibenzo-p-dioxins (PCDDs), polychlorodibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (dl-PCBs) is chromatography with high resolution mass spectrometry (GC-HRMS). It is interesting to check the suitability of screening methods that are faster and less expensive. Different matrices (milk, fish oil, chicken compound feed, pork tissue, chicken tissue, sepiolitic clay, whole egg and herring tissue) were analysed in the frame of the European project DIFFERENCE1. One of the aims of this project is to optimise screening methods. The CALUX bio-assay was one of the screening techniques used. This paper presents the extraction and purification methods used for the analyses. The CALUX results for dioxins and for dl-PCBs were compared to the corresponding GC-HRMS results.

2004-09-15