lactobacillus spp isolated: Topics by WorldWideScience.org

Probiotic Lactobacillus spp. Diminish Helicobacter hepaticus-Induced Inflammatory Bowel Disease in Interleukin-10-Deficient Mice

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1998-06-01

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Predominant and common cause of urinary tract infection(s) in sukkur city

Clinical and experimental evidence has demonstrated the potential role of probiotics in the prevention or treatment of inflammatory bowel disease. Probiotic clones with direct immunomodulatory activity...Full Text Available

2005-02-01

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Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat.

To examine and evaluate the predominant and common etiologic agent(s) of urinary tract infection (UTI) in Sukkur city and to determine their current antibiotic susceptibility/resistance trends. Nine hundred sixty six patients out of 1430 abnormal urine reports (showing significant abnormalities such as protein, pus, red cells) were asked to give second sample. The urine samples were examined by both urine test strip (Combur 10 Test, Roche) and microscopically (wet preparation) followed by culture and sensitivity. The culture sensitivity was done on urine samples of which bacterial numbers were estimated as more than 105CFU. The study was conducted at Safeway Diagnostic and Research Laboratory Sukkur and the Department of Microbiology, Shah Abdul Latif University Khairpur, Sindh from March 2001 to March 2003. Eight hundred fifty-two (88.2%) showed Escherichia spp followed by Klebsiella spp (6.9%) and proteus spp (4.9%). ...

2001-03-01

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A rapid and simple DNA extraction procedure to detect Salmonella spp. and Listeria monocytogenes from fresh produce using real-time PCR

In acute liver failure following hepatitis, toxic insults, or after major liver surgery, there is an increased bacterial translocation from the gut. This may explain some of the infectious complications seen in these conditions. To elucidate mechanisms and find possible preventive measures, we investigated the effect of rectal administration of arginine and probiotic bacteria (Lactobacillus spp.) on bacterial translocation and the extent of liver failure. Sprague-Dawley rats were used and five different Lactobacillus strains (Lb. reuteri R2LC, Lb. rhamnosus DSM 6594 (= strain 271), Lb. plantarum DSM 9843 (= strain 299v), Lb. fermentum 8704:3 (= strain 245), and Lb. reuteri (= strain 108) were administered rectally once daily for 8 days with and without 2% arginine. Acute liver injury (ALI) was induced on the eighth day by intraperitoneal injection of D-galactosamine (1.1 g/kg body weight), and samples were collected after ...

1997-03-01

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Pathogenic and nonpathogenic Acanthamoeba spp. in thermally polluted discharges and surface waters

DNA isolation procedures significantly influence the outcome of PCR-based detection of human pathogens. Unlike clinical samples, DNA isolation from food samples such as fresh and fresh-cut produce has remained a formidable task and has hampered the sensitivity and accuracy of molecular methods. We...

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Growth promotion and induction of resistance in tomato plant against Xanthomonas euvesicatoria and Alternaria solani by Trichoderma spp.

During spring and autumn, the total number of amoebae and the number of acanthamoeba species able to grow at 37 degrees C were determined in six thermally polluted factory discharges and the surrounding surface waters. The isolated Acanthamoeba strains were studied for growth in axenic medium, cytopathic effect in Vito cell cultures, and virulence in mice. Although more amoebae were isolated in autumn, the number of Acanthamoeba species was lower than in spring, when the percent of pathogenic strains among the isolates was highest. Higher concentrations of amoebae were found in warm discharges, and more virulent strains occurred in thermal discharges than in surface waters.

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British Library Electronic Table of Contents (United Kingdom)

In tomato crop, the induction of resistance emerges as an important alternative for achieving the reduction of chemicals in disease control. This study aimed to evaluate the ability of 28 Trichoderma isolates to promote the growth of tomato seedlings and to induce systemic resistance (ISR) against Xanthomonas euvesicatoria and Alternaria solani, the causal agents of bacterial spot and early blight, respectively. Twelve isolates promoted the increase of plant dry matter mass (DMM) above 100%, showing the great potential of these strains. All isolates were able to colonize the root system of tomato plants. The plant growth-promoting isolates were further evaluated for potential elicitation of ISR. Treatment of the soil with all Trichoderma isolates provided protection in tomato plants from 2...

2011-01-01

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The characterization of undecaprenol of Lactobacillus plantarum

Infection by mycotoxigenic fungal species and mycotoxin contamination of maize grain in Umbria, central Italy

Evidence for the presence of undecaprenol in the unsaponifiable lipid of Lactobacillus plantarum (N.C.I.B. 6376) is presented. Characterization of the compound was based mainly on...Full Text Available

1970-06-01

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British Library Electronic Table of Contents (United Kingdom)

Surveys were carried out in 2006 and 2007 in Umbria (central Italy) to evaluate the presence of mycotoxigenic fungi and mycotoxins in maize grain sampled at harvest. Fusarium spp., were the most abundant species detected in maize kernels, followed by Aspergillus species of sections Flavi and Nigri and by Penicillium spp. Among Fusarium species, F. verticillioides was the most prevalent species, as detected by PCR directly on the kernels and on the fungi isolated from the kernels, followed by F. proliferatum and F. subglutinans. Fumonisins were the predominant mycotoxins with values, on average, of 4.3 and 5.7mgkg-1, in 2006 and 2007, respectively, with a maximum of 76.3mgkg-1 in the second year. Deoxynivalenol ranged from 0.2 to 3.98mgkg-1 in 2006 (average 1.04mgkg-1) and from undetectable...

2011-01-01

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Properties of ${\\beta}$-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27

Effects of Single or Mixed Supplements of Plant Extract, Fermented Medicinal Plants and Lactobacillus on Growth Performance in Broilers

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2007-03-01

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Isolation and molecular identification of small ruminant mycoplasmas in Jordan

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2007-09-01

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British Library Electronic Table of Contents (United Kingdom)

Mycoplasma infections of small ruminants are known to exist in the Mediterranean region, Asia, Africa and cause significant economic impacts but little is known of the Mycoplasma spp. in sheep and goats in the Middle East. During the period of 2002-2003, 104 flocks of local sheep and goats (17 sheep, 27 goat and 60 mixed flocks) were surveyed for the occurrence of mycoplasma infections in Northern Jordan. The clinical signs seen in the studied flocks were, to varying degrees, mastitis in sheep and goats, arthritis, mainly in kids, and pneumonia in both sheep and goats of most age groups. Small ruminant farms were sampled and pooled milk samples and nasal swabs were collected for culture and isolation of mycoplasma. Mycoplasmas were isolated from 17 (26%) of the 62 milk samples and 12 (3.9%...

2006-01-01

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Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

British Library Electronic Table of Contents (United Kingdom)

A 4.1?kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen Diaporthe perjuncta. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different Diaporthe spp. The transfected species included three D. ambigua isolates and an unidentified Phomopsis asexual state of a Diaporthe sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DR...

2011-01-01

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Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

Generation of a proton motive force by histidine decarboxylation and electrogenic histidine/histamine antiport in Lactobacillus buchneri.

Analyses of food-borne pathogens are of great importance in order to minimize the health risk for customers. Thus, very sensitive and rapid detection methods are required. Current conventional culture techniques are very time consuming. Modern immunoassays and biochemical analysis also require pre-enrichment steps resulting in a turnaround time of at least 24 h. Biomagnetic separation (BMS) is a promising more rapid method. In this study we describe the isolation of high affine and specific peptides from a phage-peptide library, which combined with BMS allows the detection of Salmonella spp. with a similar sensitivity as that of immunomagnetic separation using antibodies.

2007-04-15

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Assay for the enzymatic conversion of indoleacetic acid to 3-methylindole in a ruminal Lactobacillus species.

Lactobacillus buchneri ST2A vigorously decarboxylates histidine to the biogenic amine histamine, which is excreted into the medium. Cells grown in the presence of histidine generate both a transmembrane...Full Text Available

1993-05-01

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Exotic Small Mammals as Potential Reservoirs of Zoonotic Bartonella spp.

An assay to measure the rate of enzymatic formation of 3-methylindole (3MI) from indoleacetic acid (IAA) in Lactobacillus sp. strain 11201 was developed. The reaction mixture contained 50 micrograms...Full Text Available

1990-03-01

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Classification of Artemisia spp. Collections Based on Morphological Characters and RAPD Analysis

To evaluate the risk for emerging human infections caused by zoonotic Bartonella spp. from exotic small mammals, we investigated the prevalence of Bartonella spp. in...Full Text Available

2009-04-01

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Identification of unusual Campylobacter-like isolates from poultry products as Helicobacter pullorum

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2005-12-01

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Relationship between Legionella spp. and antibody titres at a therapeutic thermal spa in Portugal.

Twenty-six unclassified Campylobacter-like strains previously isolated from 15 chicken carcasses and caecal contents, together with two more strains isolated from chicken faeces on a different occasion, were identified as Helicobacter pullorum using various phenotypic identification methods. API Campy identification kits and a 16-test identification scheme developed for campylobacters failed to identify these bacteria, or identified them as Campylobacter spp. Eighteen strains (including the two isolated on a different occasion) were chosen for examination using a more comprehensive probabilistic identification scheme. Using this method, 14 of the 18 strains were identified as H. pullorum with ID scores > 95%; two strains were also identified as H. pullorum with lower ID scores. Of the remaining two strains, one was not identified with this scheme and the other was misidentified to the H, acinonyx ...

1998-01-01

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Detection and Differentiation of Cryptosporidium spp. in Human Clinical Samples by Use of Real-Time PCR^?

The presence of Legionella spp. in the water of a Portuguese spa was ascertained during the spa season, between May and November. Simultaneously the prevalence of anti-legionella antibodies in people...Full Text Available

1995-08-01

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Expansion of Phragmites australis (Cav.) Trin. ex Steud. (Common Reed) into Typha spp. (Cattail) Wetlands in Northwestern Indiana, USA

Real-time PCR has the potential to streamline detection and identification of Cryptosporidium spp. in human clinical samples. In the present...Full Text Available

2011-03-01

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First report of the complete sequence of Sida golden yellow vein virus from Jamaica.

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2009-06-01

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Aflatoxin-producing Aspergillus spp. and aflatoxin levels in stored cassava chips as affected by processing practices

Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, Cuba and Florida in ...

2011-05-29

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Metabolic Engineering of Lactobacillus plantarum for Production of l-Ribulose?

Cassava chips (cassava balls, and cassava pellets) are derived cassava products traditionally produced by farmers in sub-Saharan Africa following fermentation, and drying of fresh roots of cassava, and are widely consumed in Cameroon. Once produced, this food commodity can be stored for more than two months and contaminated by a wide array of harmful microbes. In order to assess persistence of toxigenic fungi in cassava chips, aflatoxin-producing fungi (Aspergillus flavus, Aspergillus nomius, and Aspergillus parasiticus) and aflatoxins were contrasted at regular intervals in home-stored cassava chips collected in two locations of southern Cameroon throughout a two-month monitoring period. Three hundred and forty-six isolates of aflatoxin-producing fungi were found to be associated with all samples. A. flavus contaminated more samples in both types of chips (267 isolates in 53 samples), followed by A. nomius (58 isolates in ...

2009-01-01

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Phylogenetic Relationships in Pterodroma Petrels Are Obscured by Recent Secondary Contact and Hybridization

l-Ribulose is a rare and expensive sugar that can be used as a precursor for the production of other rare sugars of high market value such as l-ribose. In this work we describe a production...Full Text Available

2007-11-01

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Neurobrucellosis in Stranded Dolphins, Costa Rica

The classification of petrels (Pterodroma spp.) from Round Island, near Mauritius in the Indian Ocean, has confounded researchers since their...Full Text Available

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Helminth parasites in faecal samples from the endangered Iberian lynx (Lynx pardinus)

Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. Brucella...Full Text Available

2008-09-01

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British Library Electronic Table of Contents (United Kingdom)

The Iberian lynx is the most endangered felid in the world. Enteropathogens may threaten its survival, and therefore we analysed faecal samples from 66 different individuals (37 males and 29 females), the largest population representation studied to date. The samples were obtained from November 2005 to October 2008 in the two areas where the Iberian lynx survives: Sierra Morena and Donana (Andalusia, southern Spain). A total of 56.1% samples were parasitized with at least 6 species of helminths, including two cestodes (Hymenolepis spp. and Taenia spp.) and four Nematodes (Ancylostoma spp., Toxocara spp., Toxascaris leonina, and Capillaria sp.). In this work, the presence of Hymenolepis is reported for the first time in Lynx pardinus. The relevance of our findings is discussed focussed on t...

2011-01-01

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Citrate Fermentation by Lactococcus and Leuconostoc spp

Impact of Bifidobacterium animalis subsp. lactis BB-12 and, Lactobacillus acidophilus LA-5-containing yoghurt, on fecal bacterial counts of healthy adults

Citrate and lactose fermentation are subject to the same metabolic regulation. In both processes, pyruvate is the key intermediate. Lactococcus lactis subsp. lactis...Full Text Available

1991-12-01

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British Library Electronic Table of Contents (United Kingdom)

This randomized, placebo-controlled, double blind, parallel dose-response study investigated the impact of 4-week commercial yoghurt consumption supplemented with Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus acidophilus (LA-5) on fecal bacterial counts of healthy adults. Fifty-eight volunteers were randomly assigned to three different groups: 1. placebo (no probiotic, no starter and no green tea extract); 2. Yoptimal (10^9cfu/100g of BB-12 and LA-5 and 40mg of green tea extract) and 3. Yoptimal-10 (10^1^0cfu/100g of BB-12, 10^9cfu/100g of LA-5 and 40mg of green tea extract). These yoghurt products also contained Lactobacillus delbrueckii subsp. bulgaricus (10^7cfu/100g) and Streptococcus thermophilus (10^1^0cfu/100g). The quantitative PCR (qPCR) results showed that ther...

2011-01-01

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Homo-d-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum

British Library Electronic Table of Contents (United Kingdom)

In order to achieve efficient d-lactic acid fermentation from a mixture of xylose and glucose, the xylose-assimilating xylAB operon from Lactobacillus pentosus (PXylAB) was introduced into an l-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum (?ldhL1-xpk1::tkt-?xpk2) strain in which the phosphoketolase 1 gene (xpk1) was replaced with the transketolase gene (tkt) from Lactococcus lactis, and the phosphoketolase 2 (xpk2) gene was deleted. Two copies of xylAB introduced into the genome significantly improved the xylose fermentation ability, raising it to the same level as that of ?ldhL1-xpk1::tkt-?xpk2 harboring a xylAB operon-expressing plasmid. Using the two-copy xylAB integrated strain, successful homo-d-lactic acid production was achieved from a mixture of 25?g/l xylos...

2011-01-01

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Envirocentre - Case Studies

Wastenet

... Most of the received offers were ecolabelled cleaning products and were not more expensive than conventional products. Case 3: The procurement of sustainable cleaning services 104kB Eight cooperating municipalities in the Netherlands needed a new contractor for cleaning services. They wanted to use SPP criteria as well as finding a social employment organisation. SPP criteria for cleaning services were used to set minimum requirements in ...

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Campylobacter jejuni, C. coli, and C. lari naturally present in Leghorn laying hens and the antibiotic resistance profiles of these organisms

Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium

Campylobacter spp. are present in the intestinal tract and internal tissues of broiler breeder and broiler chickens. Campylobacter spp. are known to cause acute bacterial gastroenteritis in humans and raw poultry products have been implicated as a significant source of these infections. The object...

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British Library Electronic Table of Contents (United Kingdom)

Abstract Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and...

2008-01-01

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Succession of Indigenous Pseudomonas spp. and Actinomycetes on Barley Roots Affected by the Antagonistic Strain Pseudomonas fluorescens DR54 and the Fungicide Imazalil

Production of a fecal mutagen by Bacteroides spp.

In recent years, the interest in the use of bacteria for biological control of plant-pathogenic fungi has increased. We studied the possible side effects of coating barley seeds with the antagonistic...Full Text Available

2001-03-01

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Non-agonistic familiarity decreases aggression in male Turkish hamsters, Mesocricetus brandti

Forty species of anaerobes were screened for the ability to produce an ether-extractable mutagen which is present in the feces of 15 to 20% of individuals in populations at high risk for colon cancer....Full Text Available

1982-09-01

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Identification of a Chemically Induced Point Mutation Mediating Herbicide Tolerance in Annual Medics (Medicago spp.)

In laboratory studies, hamsters (Mesocricetus spp.) exhibit intense male-male aggression, thus making them an excellent model system for studies of the functional and mechanistic...Full Text Available

2009-02-01

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Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae

Background and AimsSulfonylurea (SU) herbicides are used extensively in cereal–livestock farming zones as effective and cheap herbicides with useful levels of residual activity....Full Text Available

2008-05-01

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Diurnal variation of phytoplankton community in a high frequency area of HABs: Daya Bay, China

BackgroundOncidium spp. produce commercially important orchid cut flowers. However, they are amenable to intergeneric and inter-specific crossing making phylogenetic...Full Text Available

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British Library Electronic Table of Contents (United Kingdom)

Phytoplankton community was investigated in the cage culture area of Daya Bay during a diurnal cycle. Two rainfalls occurred during the course of the experiment and decreased the surface seawater salinity in the aquaculture area. A total of 38 species were identified, of which the dominant species included Pseudo-nitzschia spp. and Skeletonema costatum. Water stratification obstructed the vertical migration of dinoflagellates. Statistical analysis indicated that Synechococcus showed negative relationship with silicate and ammonia, which indicated that Synechococcus adapted to grow at oligotrophic environment. Phytoplankton community structure implied the risk of Pseudo-nitzschia spp. blooms in the aquaculture area of Daya Bay.

2011-01-01

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Probiotics inhibit enteropathogenic E. coli adherence in vitro by inducing intestinal mucin gene expression.

Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study.

Probiotic agents, live microorganisms with beneficial effects for the host, may offer an alternative to conventional antimicrobials in the treatment and prevention of enteric infections. The probiotic agents Lactobacillus plantarum 299v and Lactobacillus rhamnosus GG quantitatively inhibited the adherence of an attaching and effacing pathogenic Escherichia coli to HT-29 intestinal epithelial cells but did not inhibit adherence to nonintestinal HEp-2 cells. HT-29 cells were grown under conditions that induced high levels of either MUC2 or MUC3 mRNA, but HEp-2 cells expressed only minimal levels of MUC2 and no MUC3 mRNA. Media enriched for MUC2 and MUC3 mucin were added exogenously to binding assays and were shown to be capable of inhibiting enteropathogen adherence to HEp-2 cells. Incubation of L. plantarum 299v with HT-29 cells increased MUC2 and MUC3 mRNA expression levels. From these in vitro studies, we propose the hypothesis that the ...

1999-04-01

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Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study

The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time. PMID:18762999

2008-09-02

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British Library Electronic Table of Contents (United Kingdom)

The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time.

2009-01-01

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Oral 6R-BH4 for the Treatment of Isolated Systolic Hypertension and Endothelial Dysfunction

Novel strategy for isolating suppressors of meiosis-deficient mutants and its application for isolating the bcy1 suppressor

Isolated Systolic Hypertension; Endothelial Dysfunction

2008-12-04

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Full Text Available

1997-03-01

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None - TDGLOBAL - NASA

Isolation and Identification of Mycoplasmas

vibration Isolation system for the camera. ... to further define the response of the camera vibration isolation system. These ...

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International Science & Technology Center (ISTC)

Isolation and Identification of Mycoplasmas, Development of Reliable Antigen for Mycoplasmas Detection and Oil-Based Vaccines

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A NUCLEOSIDE ISOLATED FROM THE VENOM OF ...

Activity of Citrus Essential Oils against-Escherichia coli-O157:H7 and-Salmonella-spp. and Effects on Beef Subprimal Cuts under Refrigeration

... Accession Number : AD0603453. Title : A NUCLEOSIDE ISOLATED FROM THE VENOM OF BUNGARUS MULTICINCTUS,. ...

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British Library Electronic Table of Contents (United Kingdom)

Abstract:- Escherichia coli-O157:H7 and-Salmonella-spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the United States. During beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. The objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (CEOs) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing, or during fabrication. Brisket flats (used to simulate beef subprimals) were spot inoculated with approximately 6 log of surrogate generic-E. coli-cocktail (previously shown to be identical in growth and survival parameters to-E. coli-O157:H7 and-Salmonella-spp.). Following drying, CEOs...

2011-01-01

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Root Hairs Play a Key Role in the Endophytic Colonization of Olive Roots by Pseudomonas spp. with Biocontrol Activity

British Library Electronic Table of Contents (United Kingdom)

The use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. The earliest steps of olive root colonization by Pseudomonas fluorescens PICF7 and Pseudomonas putida PICP2, effective biocontrol agents (BCAs) against Verticillium wilt of olive (Olea europaea L.) caused by the fungus Verticillium dahliae Kleb., are here described. A gnotobiotic study system using in vitro propagated olive plants, differential fluorescent-protein tagging of bacteria, and confocal laser scanning microscopy analysis have been successfully used to examine olive roots?Pseudomonas spp. interactions at the single-cell level. In vivo simultaneous visualization...

2011-01-01

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Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences

British Library Electronic Table of Contents (United Kingdom)

Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered as the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. In the endemic regions of the North of Iran, Fasciola hepatica and Fasciola gigantica have been previously characterized on the basis of morphometric differences, but the use of molecular markers is necessary to distinguish exactly between species and intermediate forms. Samples from buffaloes and goats from different localities of northern Iran were identified morphologically and then genetically characterized by sequences of the first (ITS-1) and second (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA). Comparison of the ITS of the northern Iranian samples ...

2011-01-01

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Digital Libraries: From Process Modelling to Grid-based Service Oriented Architecture

CERN Document Server

Graphical Business Process Modelling Languages (BPML) like Role Activity Diagrams (RAD) provide ease and flexibility for modelling business behaviour. However, these languages show limited applicability in terms of enactment over distributed systems paradigms like Service Oriented Architecture (SOA) based grid computing. This paper investigates RAD modelling of a Scientific Publishing Process (SPP) for Digital Libraries (DL) and tries to determine the suitability of Pi-Calculus based formal approaches to enact SOA based grid computing. In order to achieve this purpose, the Pi-Calculus based formal transformation from a RAD model of SPP for DL draws attention towards a number of challenging issues including issues that require particular design considerations for appropriate enactment in a SOA based grid system.

2006-01-01

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Antiprotozoal and cytotoxic activities in vitro of Colombian Annonaceae

British Library Electronic Table of Contents (United Kingdom)

Ethnobotanical and chemotaxonomical studies for antiparasitic activity of Colombian Annonaceae were carried out. In vitro antiprotozoal activity of 36 extracts obtained from six different species was determined against promastigotes of three Leishmania species, epimastigotes of Trypanosoma cruzi and both chloroquine sensitive (F32) and resistant (W2) Plasmodium falciparum. Cytotoxic activity was evaluated in U-937 cells. Active extracts were selected according their selectivity index (SI). Extracts from Annona muricata, Rollinia exsucca, Rollinia pittieri and Xylopia aromatica were active against Leishmania spp. and Trypanosoma cruzi showing IC50 values lower than 25mg/ml. Hexane extract from Rollinia pittieri leaves was the most selective against Trypanosoma cruzi and Leishmania spp. (IS=...

2007-01-01

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VIBRATION ISOLATION OF SATELLITE TAPE RECORDERS

The role of the HCR system in the repair of lethal lesions of Bacillus subtilis phages and their transfecting DNA damaged by radiation and alkylating agents

A standardized method of shock and vibration isolation for satellite tape recorders has been developed. Using readily avail- able components, the isolators ...

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Separation and Measurement of Plant Alkaloid Enantiomers by RP-HPLC Analysis of their Fmoc-Alanine Analogs

The role of the HCR system in the repair of prelethal lesions induced by UV light, #gamma# radiation and alkylating agents was studied in the Bacillus subtilis SPP1 phage, its heat sensitive mutants (N3, N73 nad ts_1) and corresponding infectious DNA. The survival of phages and their transfecting DNA after treatment with UV light is substantially higher in hcr"+ cells than in hcr cells, the differences being more striking in intact phages than in their transfecting DNA's. Repair inhibitors reduce survival in hcr"+ cells: caffeine lowers the survival of UV-irradiated phage SPP1 in exponentially growing hcr"+ cells but has no effect on its survival in competent hcr"+ cells; acriflavin and ethidium bromide decrease the survival of the UV-irradiated SPP1 phage in both exponentially growing and competent hcr"+ cells to the level of survival observed in hcr cells; moreover, ethidium bromide lowers the number of infective centres ...

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Live Brucella spp. fail to induce tumor necrosis factor alpha excretion upon infection of U937-derived phagocytes.

Plants often synthesize secondary metabolites that are enantiomers. Enantiomers can cause very different physiological responses. Ammodendrine (1) and anabasine (2) are teratogens that can cause congenital malformations in livestock and enantiomeric forms of each have been found in Lupinus spp. an...

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High Prevalence of Ceftazidime-Resistant Klebsiella pneumoniae and Increase of Imipenem-Resistant Pseudomonas aeruginosa and Acinetobacter spp. in Korea: a KONSAR Program in 2004

Tumor necrosis factor alpha (TNF-alpha) plays a central role in activation of first-line defenses of a host against foreign organisms. To determine whether Brucella infection modulated TNF-alpha production,...Full Text Available

1994-12-01

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Probiotic-Induced Priming of Innate Immunity to Protect Against Rotaviral Infection

A nationwide antimicrobial resistance surveillance has been conducted since 1997 in Korea. In this study, susceptibility test data generated in 2004 by KONSAR group hospitals were analyzed and compared...Full Text Available

2006-10-31

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British Library Electronic Table of Contents (United Kingdom)

Understanding of probiotic-induced regulatory gene expression and networking is critical to further explore their roles in controlling infection. Transcriptional profile of selected innate immune genes in primary bovine intestinal epithelial cells was assessed over a time course of incubation with the probiotic Lactobacillus plantarum 299v. Based on gene expression results, a time point was chosen to prime epithelial cells with the probiotic prior to infection with rotavirus. Plaque assays and genomic analysis provided the basis for establishing the efficacy of probiotics in preventing a rotaviral infection. Plaque assays revealed that the probiotic is capable of decreasing (at least by 100-fold) the levels of live virus when the cells were primed with the probiotic. Results from gene expr...

2010-01-01

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In vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens

British Library Electronic Table of Contents (United Kingdom)

The present study describes the screening of five lactic acid bacteria (LAB) for use as probiotics based on their competitive adhesion and production of antagonistic substances against some fish pathogens. A reduction of adhesion of all pathogenic strains tested was obtained with three of the LAB strains (Lactococcus lactis subsp. lactis CLFP100, Lactococcus lactis subsp. cremoris CLFP102 and Lactobacillus curvatus CLFP150). With the exception of fish pathogens Flavobacterium psychrophilum and Renibacterium salmoninarum that were not inhibited by LAB strains, production of antagonistic compounds by all tested LAB was observed against at least one of the indicator strains. Based on mucus adhesion, competitive exclusion, and suppression of fish pathogen growth, the selected LAB strains can b...

2007-01-01

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61

Food-grade gene expression in lactic acid bacteria

British Library Electronic Table of Contents (United Kingdom)

Abstract In the 1990s, significant efforts were invested in the research and development of food-grade expression systems in lactic acid bacteria (LAB). At this time, Lactococcus lactis in particular was demonstrated to be an ideal cell factory for the food-grade production of recombinant proteins. Steady progress has since been made in research on LAB, including Lactococcus, Lactobacillus and Streptococcus, in the areas of recombinant enzyme production, industrial food fermentation, and gene and metabolic pathway regulation. Over the past decade, this work has also led to new approaches on chromosomal integration vectors and host/vector systems. These newly constructed food-grade gene expression systems were designed with specific attention to self-cloning strategies, food-grade selection...

2011-01-01

62

Bioconvertion of spent cellulose sausage casings

British Library Electronic Table of Contents (United Kingdom)

Cellulose sausage cellulose casings are used extensively in the manufacture of sausages in meat packaging. After stripping the meat, spent casings mainly contain cellulose and residual meat juice with salt, nitrate and nitrite. Disposal of spent sausage casings has serious economic and environmental concerns for the sausage industry. This work describes bioconversion of spent cellulose casings (SCC) into enzymes, lactic acid and ethanol by using cellulolytic fungi, lactobacillus and yeasts. The solid substrate cultivation (SSC) of Trichoderma reesei RUT C-30 on SCC and blends gave a maximum of 152 filter paper cellulase (FPase) activity and about 100 carboxymethylcellulase activity (CMCase)/g dry weight substrate. The SSC produced enzyme-rich casing with 50 FPase when directly mixed as suc...

2008-01-01

63

A lytic enzyme cocktail from Streptomyces sp. B578 for the control of lactic and acetic acid bacteria in wine

British Library Electronic Table of Contents (United Kingdom)

Beside yeasts, lactic acid bacteria (LAB) are the most abundant microbes in must during vinification. Whereas Oenococcos oeni is commercially used as a starter culture for the biological acid reduction in wines, other species are responsible for different types of wine spoilage. Members of the genera Pediococcus, Weissella, Leuconostoc, and Lactobacillus are producers of exopolysaccharide slimes, biogenic amines, acetic acid, and other off-flavors. In order to control microbial growth, different procedures such as heating of must and addition of sulfite or lysozyme from egg white are generally applied. Yet, because of health risks, the application of sulfite should be reduced and lysozyme is not effective against all LAB. In this study, we describe exoenzymes from a Streptomyces sp. strain...

2009-01-01

64

Isolation of Hepatoprotective Phenylpropanoid from Lactuca indica

Identification of CTX-M-14 Extended-Spectrum ?-Lactamase in Clinical Isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea

Full Text Available

2010-03-01

65

Arabinogalactans isolated from the medicinal plant Maytenus ilicifolia

CTX-M-14 β-lactamase was identified in a stool isolate of Shigella sonnei and in blood isolates of Escherichia coli (one isolate) and Klebsiella pneumoniae...Full Text Available

2001-10-01

66

An index for the global continuation of relatively isolated sets of periodic orbits.

No abstract prepared.

2006-07-01

67

A simple and reliable method for obtaining Entomosporium monoconidial isolates.

Not Available

1983-01-01

68

Supercritical fluid extraction from dried banana peel (Musa spp., genomic group AAB): Extraction yield, mathematical modeling, economical analysis and phase equilibria

Full Text Available

2011-04-01

69

British Library Electronic Table of Contents (United Kingdom)

Supercritical fluid extraction from dried banana peel (Musa spp., subgroup Prata, genomic group AAB, popularly known in Brazil as Enxerto) was studied. The aspects investigated were: overall extraction curve (OEC), mass transfer modeling of the yield curves, economical analysis of the process and phase equilibrium data for the pseudo-ternary system of banana peel extract, carbon dioxide and ethanol. The extraction operating conditions evaluated were: pressure ranging from 100bar to 300bar, temperature from 40 to 50^oC and constant solvent flow rate of 5.0gCO2/min. Experimental extraction data were correlated using three kinetic models based on mass transfer equations (logistic, diffusion and Esquivel models). Phase equilibrium measurements were performed using pressure from 64.9bar to 239....

2010-01-01

70

Nutrient regulation of the saprotroph to parasite transition in Pochonia chlamydosporia, a soil microbial inoculant for nematode control

Environmental Research Database

DescriptionRoot-knot nematodes (Meloidogyne spp.) are major nematode pests of most tropical crops, making roots less efficient at withdrawing nutrients and water from soil, sometimes causing the total failure of crops grown by resource-poor farmers in Africa. Nematicides are some of the most toxic products used in crop protection, and are inappropriate or too expensive for use on most crops in Africa and there is an urgent need for new methods of nematode management. The fungus Pochonia chlamydosporia is [continued...

2011-01-31

71

First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil

Virulence Factors of Escherichia coli Isolates That Produce CTX-M-Type Extended-Spectrum ?-Lactamases

The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

2004-01-01

72

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen¹

This study determined the phylogenetic groups and virulence factors of 37 Escherichia coli isolates producing types of CTX-M compared with those of 19 isolates producing different types...Full Text Available

2005-11-01

73

Streptococcus pneumoniae Isolates with Reduced Susceptibility to Ciprofloxacin in Spain: Clonal Diversity and Appearance of Ciprofloxacin-Resistant Epidemic Clones

Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism...Full Text Available

1974-09-01

74

Sensitivity of Freshly Isolated Bacterial Pathogens to Certain Antibiotics and Nitrofurazone

Analysis of the pulsed-field gel electrophoretic profiles of 82 pneumococcal isolates with reduced susceptibility to ciprofloxacin (RSC) and of 90 co-occurring susceptible isolates indicates a considerable...Full Text Available

2001-10-01

75

Isolation and Characterization of an H₂-Oxidizing Thermophilic Methanogen

The results of antibiotic tests on bacterial pathogens freshly isolated from avian tissues and bovine milk are presented. Coliform isolates from the avian species showed an increased resistance to...Full Text Available

1969-01-01

76

Characterization of Toxin Plasmids in Clostridium perfringens Type C Isolates?

A thermophilic methanogen was isolated from enrichment cultures originally inoculated with sludge from an anaerobic kelp digester (55°C). This isolate exhibited a temperature optimum of 55 to...Full Text Available

1983-01-01

77

Association of elevated levels of extracellular neuraminidase with clinical isolates of type III group B streptococci.

Clostridium perfringens type C isolates cause enteritis necroticans in humans or necrotizing enteritis and enterotoxemia in domestic animals. Type C isolates always produce alpha toxin...Full Text Available

2010-11-01

78

Optimization for Vibration Isolation.

The level of total extracellular neuraminidase produced by 74 clinical isolates of group B streptococci isolated from diseased or asymptomatically colonized infants was assayed. Extracellular neuraminidase...Full Text Available

1978-09-01

79

Experience of isolated sleep paralysis in clinical practice in Nigeria.

An almost linear optimization problem of importance in vibration isolation has been identified and algorithms were developed to minimize the forced vibrational response of structural systems. The constraints can be either displacements of accelerations. T...

1983-01-01

80

The synergy of honokiol and fluconazole against clinical isolates of azole-resistant Candida albicans.

The supernatural fears associated with the experience of isolated sleep paralysis in the culture of developing countries is sometimes associated with the evolution of somatic symptoms of psychological...Full Text Available

1992-06-01

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81

The Acute Effect of Trimetazidine on the High Frequency Fatigue in the Isolated Rat Diaphragm Muscle

PMID:20681969

2010-07-14

82

Physiological Characterization of Starch-utilizing Saccharomyces sp. SK0704 Isolated from Kimchi

Full Text Available

2004-06-01

83

Peroxyl Radical Scavenging Capacity of Extracts and Isolated Components from Selected Medicinal Plants

Full Text Available

2008-04-01

84

Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens

Full Text Available

2010-06-01

85

Multi-loci Molecular Characterisation of Endophytic Fungi Isolated from Five Medicinal Plants of Meghalaya, India

No abstract prepared.

2007-01-01

86

Luteimonas aestuarii sp. nov., isolated from tidal flat sediment.

Full Text Available

2011-06-01

87

Isolation of deoxynivalenol-transforming bacteria from the chicken intestines using the approach of PCR-DGGE guided microbial selection.

Full Text Available

2008-10-01

88

Isolation of an acyl-CoA carboxylase from the tapeworm Spirometra mansonoides.

PMID:20576129

2010-06-24

89

Isolation and Purification of Decursin and Decursinol Angelate in Angelica gigas Nakai

PMID:697776

1978-06-14

90

Isolation and Characterization of Acinetobacter sp. BD5 Producing Lipolytic Enzyme

Full Text Available

2009-05-01

91

Genetic Characterization of Encephalomyocarditis Virus Isolated from Aborted Swine Fetus in Korea

Full Text Available

2006-07-01

92

Emulsification Activity of Acinetobacter sp. 2-3A Isolated from Petroleum Oil-Contaminated Soil

Full Text Available

2006-10-01

93

Effect of Angiotensin-I Converting Enzyme Inhibitory from Hydrolysate of Soybean Protein Isolate

Full Text Available

2009-11-01

94

Dynamic response characteristics of seismic isolation systems for building structures

Full Text Available

2010-01-01

95

Dynamic Response of Seismically Isolated High-Story Buildings according to Earthquake Records

Full Text Available

2009-08-01

96

Constituents isolated of Maytenus truncata Reiss leaves

Full Text Available

2008-10-01

97

Characterization of Lecanicillium lecanii Btab0l Isolated with Bioactivities to Tabacco Whitefly (Bemisia tabaci)

Portuguese 2006 [2 p.] Brazil Salazar, Gloria Del Carmen Melendez .

2006-05-19

98

Antioxidant, antinociceptive activity and general toxicity study of Dendrophthoe falcata and isolation of quercitrin as the major component

Full Text Available

2010-12-01

99

Analgesic Activity of Myricetin Isolated from Myrica rubra Sieb. et Zucc. Leaves

Full Text Available

2006-12-01

100

Response of seismic-isolated structures under long-period motions

Full Text Available

2009-04-01

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101

Pectinolytic yeast isolates for cold-active polygalacturonase production

In the past decade, considerable progress has been made on reducing the seismic response of structures through seismic isolation. Application of seismic-isolation techniques to nuclear power facilities is currently being investigated. This paper presents an analysis of the effect of long period motions on a seismic-isolated nuclear structure. Preliminary analysis indicates that long-period earthquake motions increase structural accelerations and relative displacements between the upper and lower mats. Relative displacement between the mats can be represented by spectral displacement at the frequency of the structure, and can be effectively reduced by increasing viscous damping in the isolator. The isolated structure behaves as a system with one degree of freedom. Future analysis of seismic effects on seismic-isolated structures should include the linear and ...

1991-08-18

102

British Library Electronic Table of Contents (United Kingdom)

Pectin rich cold stored spoiled fruits, vegetables and cold soils were screened and different pectinolytic isolates were obtained by enrichment culturing and ruthenium red plate assay. Among the primary isolates 10-15% were yeast isolates. Six isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase (PGU) production at room temperature (25 degrees C) and at 5 degrees C. One isolate identified as Saccharomyces sp. with highest polygalacturonase activity at 5 degrees C was used for enzyme production using raw fruit pectins as substrates. The isolate was identified by preliminary cultural, morphological and sugar fermentation tests. PGU production was high in raw pectin substrates like orange peel (21 U/ml), apple peel (20 U/ml ), mango peel (19 U/ml), ...

2011-01-01

103

[Aeromonas hydrophila-related septicemia in the Nile tilapia Oreochromis niloticus].

Carbon tetrachloride-induced release of calcium from isolated hepatocytes

From diseased wild and cultured Oreochromis niloticus in Lower Egypt, 17 Aeromonas hydrophila isolates were recovered. The mortality was between 10% and 70% in among cultured fish. The course of the disease ran in an acute manner. For cultured fish, the disease outbreaks were found mainly in winter and for the wild Nile fish, mortalities were observed in late spring and summer. Additionally wild fish were affected with ectoparasites. The LD50 values of the isolates ranged between 10(3) and 10(7). Isolates of high virulence were resistant to 1 hr boiling and to the bactericidal effect of fresh normal guinea pig serum. Moreover, they did not agglutinate in acriflavin. Only the virulent isolates could agglutinate tilapia erythrocytes. The above effects were reversed for avirulent isolates while moderately virulent isolates showed no consistency in their reactions. ...

1989-03-01

104

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

Studies have shown that CCl_4 administration to rats inhibits endoplasmic reticulum calcium pump activity and reduces the amount of calcium associated with subsequently isolated microsomal subcellular fractions. This report confirms that exposure of isolated hepatocytes to CCl_4 rapidly produces these effects in isolated parenchymal cells and demonstrates that when isolated hepacytes are exposed to CCl_4 calcium is rapidly released from cells. This release can be detected with a calcium ion-selective electrode when cells are incubated in a medium with low extracellular calcium. Calcium released from an intracellular pool(s) may initiate hepatotoxic changes in liver. (author).

105

British Library Electronic Table of Contents (United Kingdom)

Globodera rostochiensis and Globodera pallida are parasitic root cyst nematodes of potato (PCN), which significantly reduce yield and quality. The genetics and available molecular markers should make resistance to nematodes an excellent candidate for marker assisted selection. The study presents results of testing the suitability of known molecular markers for detection of resistance in a set of cultivars. We revealed some inconsistencies in genealogical data of the cultivars and showed inconsistent usefulness in detecting resistant cultivars. The marker TG689 was identified in almost all cultivars resistant to G. rostochiensis and together with other markers was used for verifying the resistance in a group of breeding lines. The marker TG689 was the most effective. However, the efficiency...

2011-01-01

106

NEW COLLECTION RECORDS AND HOST RANGE OF THE COTTONWOOD LEAFCURL MITE, TETRA LOBUUFERA (K!IFER) (ACARI: ERIOPHYIDAE), IN THE USA.

Microbiological quality and biophenol content of hot air-dried Thassos cv. table olives upon storage

Coyle, D.R., and J.W. Amrine, Jr. 2004. New collection records and host range of the cottonwood leafcurl mite, Tetra lobulifera (Keifer) (Acari: Eriophyidae), in the USA. Internat. J. Acarol. 30(1):3-8. The cottonwood leafcurl mite, Aculops lobuliferus Keifer, 1961, is renamed as Tetra lobulifera (Keifer). This eriophyid mite is capable of inflicting substantial damage on plantation- and native-grown cottonwoods (Populus spp.). We report new State and County collection records from the eastern and northwestern U.S.A. as well as new host records, including Populus grandidentata Michx. (big-tooth aspen), for this pest. This updates the established geographic range of T. lobulzjera, and demonstrates its ability to utilize other host plants in the genus Populus for development.

2004-01-01

107

British Library Electronic Table of Contents (United Kingdom)

Abstract Microbiological quality and biophenol content evolution was studied in minimally processed Thassos cv table olives by hot air dehydration under mild conditions (40C, 24-h, aw-=-0.893) and storage under characteristic packaging conditions (vacuum, 100% N2 and air) at 4 and 20C over a period of 180 days. No salt was used in the production line or packaging. The undesirable microorganisms (Enterobacteriaceae, Staphylococcus aureus, Bacillus, Clostridium) were undetectable until the end of the storage period. Also, modified atmospheres prevented fungal growth at both temperatures apart from the samples stored in air, in which Penicillium and Aspergillus spp. were identified. At 20C, a coexistence of mesophilic bacteria and yeasts occurred. At 4C, yeasts were the predominant microflora...

2011-01-01

108

Description of two new species and a new genus of bopyrid isopod parasites (Bopyridae: Pseudioninae) of hermit crabs from China

British Library Electronic Table of Contents (United Kingdom)

This is the first record from China of the bopyrid isopod genus Asymmetrione, of which the new species Asymmetrione globifera, a parasite of the hermit crab Dardanus hessii (Miers) in Beibu Gulf and South China Sea, is described. The female A. globifera differs from Asymmetrione nossibensis Bourdon in the shape of its barbula and ornamentation of the first oostegite. A tabulation of the occurrence of all currently known species of Asymmetrione is included. New genus Parasymmetrione is described. The type species, Parasymmetrione tuberculineata, sp. nov., a parasite of Clibanarius corallinus (H. Milne-Edwards) Xisha, is similar in appearance to Asymmetrione spp. but differs, in the female, in not having the propodi of its pereopods produced into sockets and having uniramous uropoda; the mal...

2010-01-01

109

Competitive abilities of native grasses and non-native (Bothriochloa spp.) grasses

British Library Electronic Table of Contents (United Kingdom)

Old World Bluestems (OWB), introduced from Europe and Asia in the 1920s, recently have begun to raise concerns in the Great Plains. Despite suggestion in the late 1950s that OWB were weedy and negatively impacted biological diversity, they were widely introduced throughout the Great Plains for agricultural purposes. Anecdotal evidence suggests that OWB exhibit invasive characteristics that promote competitive exclusion of native species. The objective of our study was to quantify the competitive abilities of two OWB species (Caucasian bluestem; Bothriochloa bladhii (Retz.) S.T. Blake (=?Bothriochloa caucasica (Trin.) C.E. Hubb.) and yellow bluestem; Bothriochloa ischaemum (L.) Keng) with three native grass species (big bluestem (Andropogon gerardii Vitman), little bluestem (Schizachyrium s...

2008-01-01

110

Aspects of the carbon activated production since three Pinus species; Aspectos da producao de carvao ativado a partir de tres especies de pinus SPP

A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae

Activated charcoal was produced from sawdust of Pinus caribaea var. caribaea, pinus caribaea var. honduenais and pinus cocarpa, with the objective of studying a alternative of more economic uses for forest residues and the products of the first thinning. A chemical process was utilized based on Zn Cl{sub 2} at temperature of 700 deg C and 450 deg C. It was concluded that the temperature of 700 Celsius produced a activated charcoal with better quality and greater adsorption capacity. The temperature of 450 deg C produced a material with adsorption properties for utilization where greater adsorption capacity is not needed. The production of activated charcoal is possible from the three species of tropical pinus studied. (author). 16 refs., 5 tabs

1987-12-31

111

British Library Electronic Table of Contents (United Kingdom)

Mycoplasma ovipneumoniae is considered an emerging veterinary pathogen causing pneumonia in sheep and goats worldwide. Currently it has not been possible to define a growth medium that yields the maximum growth of M. ovipneumoniae within a short incubation period. Growth yields of M. ovipneumoniae in Eatons medium are variable and not as consistently high as those seen with other Mycoplasma spp. This study investigated the ability of different M. ovipneumoniae field strains to grow in various media formulations, where PPLO broth was replaced by a vegetable protein source, and comparisons were made in terms of strain viability in Eatons medium. Studies were also conducted to determine the optimal carbohydrate source for use in the M. ovipneumoniae medium. Generally, it was found that differ...

2008-01-01

112

Genome analysis of F. nucleatum sub spp vincentii and its comparison with the genome of F. nucleatum ATCC 25586.

Evaluation of induced peanut mutant (RT-1010) for Pod yield, oil yield and resistant to aspergillus spp. invation and aflatoxin contamination

We present the draft genome sequence and its analysis for Fusobacterium nucleatum sub spp. vincentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN). A total of 441 FNV open reading frames (ORFs) with no orthologs in FN have been identified. Of these, 118 ORFs have no known function and are unique to FNV, whereas 323 ORFs have functional orthologs in other organisms. In addition to the excretion of butyrate, H2S and ammonia-like FN, FNV has the additional capability to excrete lactate and aminobutyrate. Unlike FN, FNV is likely to incorporate galactopyranose, galacturonate, and sialic acid into its O-antigen. It appears to transport ferrous iron by an anaerobic ferrous transporter. Genes for eukaryotic type serine/threonine kinase and phosphatase, transpeptidase E-transglycosylase Pbp1A are found in FNV but not in FN. Unique ABC transporters, cryptic phages, and three types of restriction-modification systems have been identified in FNV. ORFs for ...

2003-06-01

113

Whey protein isolate attenuates strength decline after eccentrically-induced muscle damage in healthy individuals

An induced mutant, RT-10 (M6 generation) was re-irradiated with 300 Gy of gamma rays. In M2 generation a mutant plant was identified, which characterized with large pods, prominent longitudinal ribbon reticulation and slight constriction. The selected mutant, RT-1010 has the highest pod yield (.3.59 t/fed.vs.2.91 and 2.61 t/fed respectively) and harvest index ( 0.52 vs. 0.42 and 0.45 resp.), the best in fancy pods percentage (57.67 % vs. 38.33 % and 44.17 % resp.), TSMK grade (98.96 % vs. 83.03 % and 84.05 % resp.), the largest pod mass (253.37 g vs . 211.32 g and 222.19 g resp.) and the highest expected oil yield (1370 Kg/fed vs. 895.44 Kg and 868.17 Kg/fed. resp.). With regard to fatty acid compositions, both the selected mutant, RT-1010 and the parental mutant RT-10 have almost a similar high oleic fatty acid (C18:1) percentage (56.35 % and 55.103 % respectively), low linoleic fatty acid (C18:2) percentage (24.20 % and 24.138 % resp.), low palmetic fatty acid (C18:0)(12.276 % and ...

2010-01-01

114

Transport of Arginine and Aspartic Acid into Isolated Barley Mesophyll Vacuoles ¹

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

115

Tick-Borne Relapsing Fever in British Columbia, Canada: First Isolation of Borrelia hermsii

The transport of arginine into isolated barley (Hordeum vulgare L.) mesophyll vacuoles was investigated. In the absence of ATP, arginine uptake was saturable with a K_m...Full Text Available

1991-10-01

116

The pattern of isolated sleep paralysis among Nigerian nursing students.

The spirochete that causes tick-borne relapsing fever, Borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern...Full Text Available

1998-12-01

117

The isolation of l(+)-methionine sulphoxide from the blowfly Phormia regina Meigen

In a cross-sectional study of the pattern of isolated sleep paralysis among the entire population of nursing students at the Neuropsychiatric Hospital in Abeokuta, Nigeria (consisting of 58 males and...Full Text Available

1992-01-01

118

Some aspects of the pharmacology of an homologous series of choline esters of fatty acids

1. l(+)-Methionine sulphoxide has been isolated for the first time from a natural source, the blowfly Phormia regina. 2. The natural compound is shown to be identical with...Full Text Available

1966-08-01

119

Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.

The pharmacological effects of a series of fatty acid-choline esters have been studied on the isolated rabbit heart, the isolated guinea-pig ileum and the rat stomach. The effect changed with increasing...Full Text Available

1957-03-01

120

Salmonella enterica in Commercial Swine Feed and Subsequent Isolation of Phenotypically and Genotypically Related Strains from Fecal Samples?

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein...Full Text Available

1992-07-01

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121

Rural Education and the Beginning Teacher.

The purpose of this study was to determine the occurrence and genotypic relatedness of Salmonella enterica isolates recovered from feed and fecal samples in commercial swine production...Full Text Available

2010-11-01

122

Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec.

This paper addresses the major issues beginning teachers face in their first postings to rural areas in Australia. The issues go beyond pedagogy. Beginning teachers need to understand social factors affecting rural communities so they can link lessons to the realities experienced by students. Geographic isolation affects the provision of education in terms of time taken to travel, cost, terrain, and technology. Rural communities have groups that are socially isolated from each other and from the staff in schools. In order to teach effectively, teachers need to be aware of the social context of the community in which they live and work. Teachers in rural areas suffer from professional isolation and often confront teaching situations for which they have had little formal preparation. Communities in remote places often move quickly to adopt technological means to overcome their isolation. Teachers in ...

1992-02-01

123

Proteome of human colon cancer stem cells: A comparative analysis

We have used restriction endonuclease analysis (REA) of genomic DNA to classify porcine Pasteurella multocida isolates with similar capsular and somatic serotypes, and to monitor the distribution of...Full Text Available

1990-10-01

124

Partial Characterization of R-Plasmids from Pasteurella multocida Isolated from Turkeys

AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.METHODS: SW1116 cells were isolated and cultured with...Full Text Available

2011-03-14

125

Numerical Taxonomy of Some Bacteria Isolated from Antarctic and Tropical Seawaters¹

Pasteurella multocida, isolated from turkeys during an outbreak of septicemic disease (“fowl cholera”), was found to be resistant to tetracycline, streptomycin, and...Full Text Available

1978-09-01

126

Multiple CTX-M-Type Extended-Spectrum ?-Lactamases in Nosocomial Isolates of Enterobacteriaceae from a Hospital in Northern Italy

Pfister, Robert M. (Lamont Geological Observatory, Palisades, N.Y.), and Paul R. Burkholder. Numerical taxonomy of some bacteria isolated from Antarctic and tropical seawaters. J....Full Text Available

1965-10-01

127

Molecular epidemiology of trimethoprim resistance among coagulase-negative staphylococci.

Twelve isolates of Enterobacteriaceae (1 of Klebsiella pneumoniae, 8 of Escherichia coli, 1 of Proteus mirabilis, and 2 of Proteus...Full Text Available

2003-09-01

128

Molecular and Kinetic Comparison of the Novel Extended-Spectrum ?-Lactamases CTX-M-25 and CTX-M-26

A 42% (70 of 167 isolates) incidence of resistance to 20 micrograms of trimethoprim per ml was found among clinical isolates of coagulase-negative staphylococci from two hospitals. A specific trimethoprim...Full Text Available

1987-11-01

129

Molecular Analyses of Salmonellaenterica Isolates from Fish Feed Factories and Fish Feed Ingredients

CTX-M-25 is a novel extended-spectrum β-lactamase isolated from a single Canadian Escherichia coli isolate. Susceptibility testing demonstrated that this enzyme confers resistance...Full Text Available

2004-12-01

130

Lizards in the ecology of salmonellosis in Panama.

Isolates of the most commonly observed salmonella serovars in Norwegian fish feed factories from 1998 to 2000 (Salmonella enterica serovar Agona, S. enterica...Full Text Available

2003-02-01

131

Legionella tucsonensis sp. nov. isolated from a renal transplant recipient.

Enteropathogenic bacteria was isolated from 131 of 447 (29.4%) neotropical Panamanian lizards belonging to 34 species of seven families. Overall, 147 strains of bacteria were isolated comprising 26...Full Text Available

1981-05-01

132

Legionella lansingensis sp. nov. isolated from a patient with pneumonia and underlying chronic lymphocytic leukemia.

A Legionella-like organism (strain 1087-AZ-H) was isolated from a pleural-fluid specimen from a renal transplant patient undergoing immunosuppressive therapy. Growth characteristics and gas-liquid chromatography...Full Text Available

1989-08-01

133

Legionella fairfieldensis sp. nov. isolated from cooling tower waters in Australia.

A Legionella-like organism, strain 1677-MI-H, was isolated from the bronchoscopy washings of a patient with pneumonia who had a 2-year history of progressive, chronic lymphocytic leukemia. The growth...Full Text Available

1992-09-01

134

Isolation of caprine arthritis encephalitis virus from goats in Mexico.

Three Legionella-like organisms were isolated from water from the cooling towers of two Australian institutions. The strains grew on buffered charcoal-yeast extract (BCYE) agar but not on BCYE agar...Full Text Available

1991-03-01

135

Isolation of Neisseria gonorrhoeae on selective and nonselective media in a sexually transmitted disease clinic.

A lentivirus was isolated from 2 goats in Mexico that were seropositive to caprine arthritis encephalitis virus (CAEV) by the agar gel immunodiffusion (AGID) test. The lentivirus was identified as CAEV...Full Text Available

1999-07-01

136

Isolation of Mycoplasma pneumoniae from the human urogenital tract.

To assess the practical significance of reported increases in the prevalence of vancomycin-susceptible strains of Neisseria gonorrhoeae on isolation of this organism, antibiotic-free chocolate agar...Full Text Available

1984-02-01

137

Isolation of Bacillus thuringiensis from Stored Tobacco and Lasioderma serricorne (F.)

Mycoplasma pneumoniae is a common etiologic agent of lower respiratory tract infections in humans. However, it has been reported previously that the organism has occasionally been isolated from sites...Full Text Available

1995-11-01

138

Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

Bacillus thuringiensis was isolated from dried tobacco residues and dead tobacco beetles (Lasioderma serricorne (F.); Coleoptera: Anobiidae) collected in a large number...Full Text Available

1994-01-01

139

Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing...Full Text Available

2011-01-01

140

Isolation and characterization of quinoline-degrading bacteria from subsurface sediments.

The isolation of an untriakontapeptide from camel pituitary extracts has been described. Its structure has been determined and shown to be identical to the sequence of carboxyl-terminal 31 amino acids...Full Text Available

1976-04-01

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141

Isolation and characterization of a new Cytophaga species implicated in a work-related lung disease.

Two gram-negative, motile bacteria isolated from deep subsurface sediments mineralized the nitrogen-containing polyaromatic hydrocarbon quinoline under aerobic conditions and transformed quinoline to...Full Text Available

1989-04-01

142

Immunoreactive properties of anti-thyroglobulin autoantibodies isolated by affinity chromatography from human thyroiditis serum.

A yellow-pigmented, gram-negative, gliding bacterium isolated from an industrial water spray air humidification system was implicated as a causative agent in several occurrences of lung disease with...Full Text Available

1984-11-01

143

High Prevalence of Extended-Spectrum Beta Lactamases among Salmonella enterica Typhimurium Isolates from Pediatric Patients with Diarrhea in China

A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed...Full Text Available

1978-02-01

144

Growth-Phase-Dependent Expression of Virulence Factors in an M1T1 Clinical Isolate of Streptococcus pyogenes

We investigated the extended-spectrum beta lactamases among 62 Salmonella enterica Typhimurium isolates recovered from children with diarrhea in a Chinese pediatric hospital. A large...Full Text Available

145

Excretion of para-aminohippurate in the isolated perfused rat kidney: net secretion and net reabsorption.

The effect of growth phase on expression of virulence-associated factors was studied by Northern hybridization in an M1T1 clinical isolate of Streptococcus pyogenes. Expression of M...Full Text Available

1999-10-01

146

Emergence of Enterobacteriaceae Isolates Producing CTX-M Extended-Spectrum ?-Lactamase in Austria

1. The excretion of para-aminohippurate (PAH) in the isolated perfused rat kidney was examined over a wide range of perfusate PAH concentrations (15 microM to 6 mM). PAH excretion increased steadily...Full Text Available

1988-03-01

147

Diversity of Extended-Spectrum ?-Lactamases and Class C ?-Lactamases among Cloacal Escherichia coli Isolates in Belgian Broiler Farms?

Among 149 extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae isolates collected from patients in southeast Austria from 1998 to 2004, 38 Escherichia coli...Full Text Available

2006-02-01

148

Development of a New Serological Test for Serotyping Haemophilus parasuis Isolates and Determination of Their Prevalence in North America

A total of 295 ceftiofur-resistant Escherichia coli isolates were obtained from 489 cloacal samples collected at five different Belgian broiler farms with the aim to evaluate the diversity...Full Text Available

2008-04-01

149

Deep Desulfurization of Diesel Oil and Crude Oils by a Newly Isolated Rhodococcus erythropolis Strain

Haemophilus parasuis causes polyserositis in swine. Fifteen serovars have been characterized by immunodiffusion test, but many field strains are not typeable. Isolates (n...Full Text Available

2004-02-01

150

Confirmation of human Campylobacter concisus isolates misidentified as Campylobacter mucosalis and suggestions for improved differentiation between the two species.

The soil-isolated strain XP was identified as Rhodococcus erythropolis. R. erythropolis XP could efficiently desulfurize benzonaphthothiophene, a complicated model sulfur compound that...Full Text Available

2006-01-01

151

Application of ribotyping for differentiating aeromonads isolated from clinical and environmental sources.

A strain from human diarrhea originally identified as Campylobacter mucosalis (NCTC 12408) was examined by using 64 phenotypic characters. The similarity of this strain to 297 isolates of Campylobacter,...Full Text Available

1994-09-01

152

Analysis of Haemophilus parasuis isolates from southern Ontario swine by restriction endonuclease fingerprinting.

We have investigated the usefulness of ribotyping for the differentiation of aeromonads isolated from five patients with gastroenteritis and from the source water, treatment plant, and distribution...Full Text Available

1992-06-01

153

An evaluation of the effectiveness of a community mentoring service for socially isolated older people: a controlled trial

To study the occurrence and distribution of various strains of Haemophilus parasuis in southern Ontario swine, organisms isolated from healthy swine, from specific pathogen-free and conventional herds,...Full Text Available

1988-07-01

154

A survey of the sensitivity of fresh clinical isolates to cefuroxime and other antibiotics.

BackgroundSocial isolation affects a significant proportion of older people and is associated with poor health outcomes. The current evidence base regarding the effectiveness of...Full Text Available

155

A Hemorrhagic Factor (Apicidin) Produced by Toxic Fusarium Isolates from Soybean Seeds

The sensitivities to cefuroxime and cephradine of potentially pathogenic bacteria isolated in two British general hospitals comprising 900 beds have been assessed. In a three-month period 2537 strains...Full Text Available

1979-09-01

156

Vasorelaxant Activities of Aqueous Extracts from Twenty Medicinal Plants Used in Oriental Medicines in Isolated Rat Aorta

Fifty-two isolates of Fusarium species were obtained from soybean seeds from various parts of Korea and identified as Fusarium oxysporum, F. moniliforme,...Full Text Available

1999-01-01

157

Synthesis of esters from lupeol isolated from Maytenus salicifolia (Celastraceae)

Full Text Available

2010-09-01

158

Shaking Table Experimental Study on 3-Dimensional Floor Isolation in Main Control Room of Nuclear Power Plant

Portuguese 2009 1 p. Brazil Magalhaes, Cassia Goncalves Silva, Gracia

159

Seismic Response Comparative Evaluation Study on Floor Isolation using LRB and FPS in Main Control Room of Nuclear Power Plant

Full Text Available

2008-02-01

160

Screening of Bacteria Producing Lipase from Insect Gut: Isolation and Characterization of a Strain, Burkholderia sp. HY-10 Producing Lipase

Full Text Available

2009-08-01

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161

Pentacyclic triterpenes lupanic isolated of branches of Maytenus imbricata

Full Text Available

2007-04-01

162

Isolation of carrier-free tantalum radioisotopes from proton-irradiated hafnium

Portuguese 2004 [1 p.] Brazil Silva, Silvia Ribeiro de Souza e Silva,

163

Induction of Apoptosis in the HepG2 Cells by HY53, a Novel Natural Compound Isolated from Bauhinia forficata

... carrier-free isotopes hafnium compounds irradiation protons solvent extraction

164

Galacturonan isolated from Maytenus ilicifolia leaves

Full Text Available

2006-08-01

165

Changes in muscular lipids in unilateral isolated hypertrophy of gastrocnemius muscle can be revealed by 1H MR spectroscopy.

English 2006 [1 p.] Brazil Uratani, JM Cipriani, TR Mellinger, CG Gorin,

2006-07-01

166

Arabinogalactans isolated from the medicinal plant Maytenus ilicifolia

Full Text Available

2009-12-01

167

A type II arabinogalactan isolated from an infusion of Maytenus ilicifolia

English 2006 [1 p.] Brazil Cipriani, TR Mellinger, CG Gorin, PAJ Sassaki,

2006-07-01

168

A new alkaloid isolated from Maytenus guianensis

English 2005 [1 p.] Brazil Cipriani, TR Mellinger, CG Sassaki, GL Gorin,

2005-07-02

169

A comparative study of the fatty acid composition of prochloron lipids

Portuguese (Jul 1981). Brazil Sousa, JR de Souza, E. de . Minas Gerais Univ.,

170

Purification and Characterization of an Extremely Thermostable Cyclomaltodextrin Glucanotransferase from a Newly Isolated Hyperthermophilic Archaeon, a Thermococcus sp.

The chemical analysis of lipids of Prochloron isolated from several hosts is discussed. The object

1983-01-01

171

Isolated dextrogastria: report of two cases

The extremely thermophilic anaerobic archaeon strain B1001 was isolated from a hot-spring environment in Japan. The cells were irregular cocci, 0.5 to 1.0 μm in diameter. The new isolate grew...Full Text Available

1999-05-01

172

Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens.

Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two distinct forms. Because of its close relationship to the liver, the right-sided stomach may produce interesting and confusing changes in the liver scan. Two cases illustrate the radiologic features of the two principal types of isolated congenital dextrogastria and the effect of one type on the liver scan.

173

A method for isolating a water influx into a well

Fifteen strains of CDC group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. Of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly...Full Text Available

1993-11-01

174

Chitinolytic enzyme production and genetic improvement of a new isolate belonging to Streptomyces anulatus

The goal of the invention is to increase the effectiveness of the isolation process through increasing the waterproofing of the stratum. The cited goal is achieved by isolating water influx into a well through introduction of a chemical reagent into the stratum. To interact with the stratum, an alloy of titanium and iron is introduced into the stratum as the chemical reagent with subsequent pumping of hydrogen into the stratum at 100 to 200C.

1983-01-01

175

British Library Electronic Table of Contents (United Kingdom)

Thirty bacterial isolates were obtained from different sources and sites at Jeddah, Saudi Arabia, on chitin agar medium; 9 of the 30 isolates were cultured in liquid medium containing chitin as sole carbon and nitrogen sources. Isolate SM21, which was isolated from shrimp shells, showed the best growth and chitinase production in liquid medium. According to its morphological, physiological and biochemical characteristics, SM21 belongs to the genus Streptomyces and was identified as Streptomyces anulatus SM21. Identification was confirmed using 16S rDNA analysis. The chitinase enzyme was precipitated with 80% NH4SO4 and purified using DEAE-cellulose ion exchange chromatography followed by Sephadex G-100 gel filtration. The molecular weight determined using sodium dodecyl sulfate polyacrylam...

2011-01-01

176

Actuarial risk of isolated CNS involvement in Ewing's sarcoma following prophylactic cranial irradiation and intrathecal methotrexate

Factors and Issues in Australian Rural Education: A Case for New Perspectives.

Records of 154 patients with Ewing's sarcoma treated at the National Cancer Institute were reviewed to assess the incidence and risk of developing isolated central nervous system (CNS) Ewing's sarcoma. Sixty-two of the 154 patients had received CNS irradiation and intrathecal (i.t.) methotrexate as part of their initial therapy to prevent the occurrence of isolated CNS Ewing's sarcoma. The risk of developing isolate CNS Ewing's sarcoma was greatest within the first two years after diagnosis and was approximately 10%. The overall risk of CNS recurrence in the group of patients receiving DNS treatment was similar to the group receiving no therapy directed to the CNS. The occurrence of isolated CNS involvement was not prevented by the use of CNS irradiation and i.t. methotrexate. Because of a lack of efficacy to the CNS irradiation regimen, current treatment regimens do not include therapy directed to CNS.

177

Tree and fruit traits of progenies from the cross between (Annona cherimola Mill.??A. squamosa L.)??A. reticulata L. and approaches for the introgression of valuable genes from A. reticulata L.

New interest by Australians in the state of rural education requires new understanding of factors affecting rural education and its related issues. Educational programs have not been flexible enough to cope with rural diversity, a key element in the milieu. Standards such as "equality of opportunity" and "uniformity" have been misapplied to rural Australian education, which is made distinctive by the inter-relatedness of many factors. Three dimensions of isolation are discussed: (1) types of isolation (geographic, cultural, social, and professional isolation); (2) ways of coping with isolation (understanding isolation, developing a sense of community, developing awareness of options, training the isolated, application, and technology); and (3) conditions affecting educational changes in isolated communities (social and political climate, ...

1982-12-01

178

British Library Electronic Table of Contents (United Kingdom)

Annona reticulata L. possesses many traits desirable in other edible annonas, and hybridization is the simplest means to combine desirable features of related species. In this study, A. reticulata was hybridized with atemoya (A. cherimola Mill.??A. squamosa L.), and 250 trispecies hybrids were studied for 28 traits (12 tree traits and 16 fruit traits) with the objective of salvaging useful genes from the three edible annonas and determining the extent of variation in the progeny. The heterozygous nature of Annona spp. and simultaneous segregation of three distant genomes resulted in a tremendous heterogeneity in the progenies. The fitness of the progenies ranged from very vigorous to very weak, with a wide range of values for tree height (1.75?5.9?m), canopy spread (1.15?5.07?m) and trunk...

2010-01-01

179

Treatment and Course of Community-Acquired Pneumonia Caused by Atypical Pathogens

British Library Electronic Table of Contents (United Kingdom)

Objetivo Estudiar la evolucion de un grupo de neumonias extrahospitalarias causadas por germenes atipicos (Mycoplasma pneumoniae, Legionella spp., Coxiella burnetii y Chlamydophila pneumoniae) en funcion del tratamiento empirico recibido. Pacientes y metodos Entre enero de 1996 y febrero de 2001 ingresaron en nuestra unidad 390 casos de neumonia, de los que 89 estaban causados por germenes atipicos. Los pacientes se dividieron retrospectivamente en 2 grupos segun el tratamiento empirico pautado: grupo A, al que se habia proporcionado cobertura frente a germenes atipicos (quinolonas o macrolidos), y grupo B, al que no se habia proporcionado dicha cobertura. Se estudio la evolucion segun las diferencias entre ambos grupos en la estancia hospitalaria, la resolucion radiologica, el reingreso e...

2006-01-01

180

Soil Redox Chemistry Limitation of Selenium Concentration in Carex Species Sedges

Induction of mutation in Trichoderma viride for conversion of natural cellulose into glucose

The trace element selenium (Se) is required in the production of enzymes that protect mammalian cells from oxidative damage due to the byproducts of aerobic respiration. Its deficiency in livestock results in the nutritional muscular dystrophy called white muscle disease. This especially affects juveniles in the preweaning period. Symptoms have also been reported in wild herbivores on low-Se forage, and their appearance may be episodic, suggesting temporal variations in Se uptake by plants. Here, we report variations in selenium concentrations in Carex spp. sedges used as forage by bighorn sheep (Ovis canadensis) on summer range in the Wyoming, Wind River Mountains, and correlate those variations with soil conditions that affect the bioavailability of selenium. Variations in available Se are explained based upon the known oxidation/reduction chemistry of the element. It is concluded that water-saturated, alpine soils provide conditions suitable for the reduction of ...

2007-09-01

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181

Characteristics of seismic ground motions and responses of base isolated buildings in Japan

The production of cellulolytic enzymes from fungi has been extensively studied. Several mutants of Trichoderma reesei were selected. Most of the studies were carried out on T. reesei, T. viride, T. harzianum, Penicillium funiculosum, Altemaria alternata. Aspergillus phoenicis, A. ustus, A. tamarii, A. japonicus, and A. niger. T. koningii is one of the most active producers of the so-called C, factor, which is indispensable for the rapid and extensive attack on crystal-line cellulose. However, Trichodenna is known to excrete only small amounts of {beta}-glucosidase. Therefore, Trichoderma is supplemented with {beta}-glucosidase from Aspergillus to increase the saccharification rate of cellulose to glucose as the main sugar. Induction of mutations in Trichodenna spp. rather than T. viride as a tool for the enhancement of {beta}-glucosidase activity was reported. Unfortunately, T. reesei is a poor producer of {beta}-glucosidase. On the other hand, T. harzianum M{sub ...

1991-12-31

182

NMR analysis of the structure of synaptobrevin and of its interaction with syntaxin

In this paper, the authors describe the characteristics of design seismic input motions and recently observed seismic records of base isolated buildings in Japan. First, we describe some seismic design spectra for building structures which were adopted. Then we show response spectra and energy spectra of observed records obtained during two recent major earthquakes (The 1995 Hyogo-ken Nanbu Earthquake and the 2003 Tokachi-oki Earthquake), which demonstrate the effectiveness of base isolation against large amplitude earthquake ground motion. We also introduce the responses of base isolated buildings during some earthquakes including the Tokachi-oki Earthquake. These data were gathered through questionnaires sent to many engineers who had conducted response observations of base isolated buildings. Last, an example of a base isolated building on soft ground is shown. We point out the ...

2007-04-26

183

beta-Lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands

Synaptobrevin is a synaptic vesicle protein that has an essential role in exocytosis and forms the SNARE complex with syntaxin and SNAP-25. We have analyzed the structure of isolated synaptobrevin and its binary interaction with syntaxin using NMR spectroscopy. Our results demonstrate that isolated synaptobrevin is largely unfolded in solution. The entire SNARE motif of synaptobrevin is capable of interacting with the isolated C-terminal SNARE motif of syntaxin but only a few residues bind to the full-length cytoplasmic region of syntaxin. This result suggests an interaction between the N- and C-terminal regions of syntaxin that competes with core complex assembly.

1999-07-15

184

Two sisters in the same dress: Heliconius cryptic species

Objectives: The purpose of this work was to study the genetic determinants responsible for extended-spectrum beta-lactamase (ESBL) resistance of Salmonella isolated from Dutch poultry, poultry meat and hospitalized humans. Methods: Thirty-four ESBL-resistant Salmonella isolates from The Netherlands were tested towards 21 antimicrobial agents. PCR and sequencing were used to determine the underlying genetic determinants responsible for the ESBL phenotypes. The transferability of the ESBL phenotypes was tested by conjugation to a susceptible Salmonella enterica serovar Dublin and plasmid purification, restriction fragment length polymorphism (RFLP) and pulsed-field gel electrophoresis (PFGE) were employed to further characterize a subset of the isolates. Results: A great genetic diversity was seen among the isolates. The bla(TEM-52) gene was most predominant and was found among Salmonella enterica ...

2005-01-01

185

The antimicrobial efficacy of a silver alginate dressing against a broad spectrum of clinically relevant wound isolates

BackgroundSister species divergence and reproductive isolation commonly results from ecological adaptation. In mimetic Heliconius butterflies, shifts in colour pattern...Full Text Available

186

British Library Electronic Table of Contents (United Kingdom)

Wound dressings impregnated with silver have a role to play in aiding to reduce both the dressing and wound microbial bioburden. It is therefore imperative that antimicrobial wound dressings have efficacy on a broad range of clinical significant microorganisms. Accordingly, this study aimed to determine the antimicrobial efficacy of a silver alginate dressing against 115 wound isolates that had been isolated routinely from patients at West Virginia University Hospital. Standardised corrected zones of inhibition (CZOIs) were performed on all clinical isolates. It was found that the silver alginate dressing was able to inhibit the growth of all microorganisms tested. In particular, the silver alginate dressing inhibited the growth of Candida albicans and yeasts with CZOI of 3-115 mm. All met...

2011-01-01

187

The Structure of Plant Cell Walls

The Effect of Experience on Male Courtship and Mating Behaviors in a Cellar Spider

The molecular structure, chemical properties, and biological function of the xyloglucan polysaccharide isolated from cell walls of suspension-cultured sycamore (Acer pseudoplatanus)...Full Text Available

1973-01-01

188

Studies of the action of hypoglycin-a, a hypoglycaemic substance

... video recorded in an isolated booth under a Panasonic WV-CP470 video camera located in an environmentally ... ...

189

Response characteristics of base-isolated structure with silicone rubber bearings

Some biological effects of hypoglycin-A, a compound isolated from the fruit of Blighia sapida, have been investigated. Administration of this compound to animals caused drowsiness...Full Text Available

1958-06-01

190

Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

More than sixty base-isolated buildings have been built in Japan. A number of base-isolation systems were considered in our research, which was intended to establish the effectiveness of base-isolation systems. We conducted research on silicone rubber bearings. Generally, silicone rubber is durable and its characteristics are not dependent on the temperature within the relevant design range. The first part of the report covers material and elements testing. After the bearings were installed in the building, we performed forced vibration tests in both the horizontal and vertical directions. These test results form the next section. After several experiments, we carried out earthquake observations. We report on the effectiveness of the system in reducing response acceleration during a small displacement. This system was installed in the building in March 1992

1993-08-15

191

Presence of Arsenic Resistance in Salmonella enterica Serovar Kentucky and Other Serovars Isolated from Poultry

Full Text Available

192

Nonchylous idiopathic pleural effusion in the newborn

... B. P. Rosen. An arsenic metallochaperone for an arsenic detoxification pump. Proc. Natl. Acad. Sci. U. S. A 103( ... ...

193

Joint Airspace Management and Deconfliction (JASMAD)

Congenital isolated pleural effusion is a rare cause of respiratory distress in neonates. It is usually chylous. Herein, we report a rare case of nonchylous congenital idiopathic pleural effusion.

2011-01-01

194

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

... problems by isolating them. 4 LAN Segmentation, http://netcert.tripod.com/ ccna/internetworking/lanseg.html, Jan 2005 Destination ...

2009-01-01

195

Isolation and differentiation of chondrocytic cells derived from human embryonic stem cells using dlk1/FA1 as a novel surface marker

The authors have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy using (/sup 32/P)-labelled nucleotides. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase.

1987-03-24

196

Interstellar PAH emission in the 11-14 micron region: new insights from laboratory data and a tracer of ionized PAHs

Udgivelsesdato: 2009-Dec

2009-01-01

197

Interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms

The Ames infrared spectral database of isolated, neutral and ionized polycyclic aromatic

1999-01-01

198

In vitro susceptibility of enterococci strains isolated from urine samples to fosfomycin and other antibiotics

BackgroundIt is well established that Legionella pneumophila is a waterborne pathogen; by contrast, the mode of Helicobacter pylori transmission...Full Text Available

199

British Library Electronic Table of Contents (United Kingdom)

Enterococci are mostly isolated from urinary tract infections (UTIs). Increasing antibiotic resistance affects the success rate in empirical treatment, thus makes antibiotic susceptibility tests important. The aim of this study was to determine the species distribution and resistance patterns of Enterococcus strains isolated from urine samples to antibacterial agents including fosfomycin in a teaching hospital, Istanbul. The identification of 100 Enterococcus strains were determined by using conventional methods and API 20 Strep (bioMerieux France). The susceptibility testing was performed by disk diffusion and Etest. The majority of isolates were E. faecalis (67%), followed by E. faecium (33%). The ratio of E. faecalis to E. faecium was 2.03/1. E. faecalis and E. faecium strains were resi...

2011-01-01

200

Genotyping of Candida albicans on the basis of polymorphisms of ALT repeats in the repetitive sequence (RPS)

British Library Electronic Table of Contents (United Kingdom)

SummaryBackgroundCandida albicans is one of the most important etiologic agents causing superficial and deep fungal infections. For prevention of candidiasis, it is important to develop a rapid system that discriminates C. albicans at the strain level.ObjectiveTo develop a system that can identify C. albicans at the strain level.MethodsGenomic DNAs were purified from 179 clinical isolates of C. albicans, and were used as templates for PCR amplification of 25S rDNA and ALT repeats in repetitive sequences (RPSs). PCR products generated from ALT repeats were digested with EcoRI and/or ClaI in order to study the relationships between restriction profiles and amplification profiles.ResultsOne hundred and seventy nine clinical isolates were grouped into genotypes A (92 isolates), B (38 isolates)...

2006-01-01

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201

Gallium Arsenide Pilot Line for High Performance ...

... used in our aluminum process include a barrier to isolate aluminum from the gold-based ohmic contacts, thereby preventing "purple plague," and a ...

1991-08-08

202

Foveomacular retinitis.

Effect of Macrolide Usage on Emergence of Erythromycin-Resistant Campylobacter Isolates in Chickens?

A group of patients is described who developed the clinical features of foveomacular retinitis. No causative factors were isolated, and all patients strongly denied any type of sun gazing. It is possible...Full Text Available

1986-11-01

203

Development of Seismic Analysis Model and Time History Analysis for KALIMER-600

In this work we conducted both in vitro and in vivo experiments to examine the development and mechanisms of erythromycin (Ery) resistance in Campylobacter jejuni and Campylobacter...Full Text Available

2007-05-01

204

Density Gradients for Isolation of Mononuclear Blood Cells for ...

This report describes a simple seismic analysis model of the KALIMER-600 sodium cooled fast reactor and its application to the seismic time history analysis. To develop the simple seismic analysis model, the detailed 3-D finite element analyses for main components, IHTS piping system, and reactor building were carried out to verify the dynamic characteristics of each part of simple seismic analysis models. By using the developed simple model, the seismic time history analyses for both cases of a seismic isolation and non-isolation design of KALIMER-600 were performed. From the comparison of the calculated floor response spectrum, it is verified that the seismically isolated KALIMER-600 reactor building shows a great performance of a seismic isolation and assures a seismic integrity.

2007-02-15

205

Degradation of malathion by salt-marsh microorganisms.

... to-continuum X-ray intensity ratio [5]. For ence in the means [7]. In addition, washing samples on thin films, the characteristic X- lymphocytes in NH ...

2011-05-15

206

Comparative activity of tigecycline and tetracycline on Gram-negative and Gram-positive bacteria revealed by a multicentre study in four North European countries

Numerous bacteria from a salt-marsh environment are capable of degrading malathion, an organophosphate insecticide, when supplied with additional nutrients as energy and carbon sources. Seven isolates...Full Text Available

1977-02-01

207

British Library Electronic Table of Contents (United Kingdom)

Abstract Background: This study involves a multicentre surveillance of tigecycline and tetracycline activity against Gram-negative and Gram-positive bacteria from primary care centres (PCCs), general hospital wards (GHWs) and intensive care units (ICUs) in Denmark (n == 9), Finland (n == 10), Norway (n == 7) and Sweden (n == 19). Methods: The hospitals were each asked to test 30 consecutive Gram-positive and 30 Gram-negative clinical isolates. Supportive information accompanying each isolate included the study centre, ward level (PCC, GHW, or ICU), patient identification and source of the isolate. Minimum inhibitory concentrations (MICs) for tetracycline and tigecycline were determined with the Etest. Results: The isolates collected comprised 1610 Gram-negative and 1767 Gram-positive clini...

2011-01-01

208

Combined Radiation and Thermal Injury after Nuclear Attack

Circulating Skeletal Stem Cells

... Except for isolated radiation accidents over the ensuing years, little practical experience has been gained in the treatment of thermal injuries ...

2011-05-13

209

Characterization of lymphocyte receptors for glycosaminoglycans.

We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are...Full Text Available

2001-05-28

210

Capsular Serotype and Antibiotic Resistance of Streptococcus pneumoniae Isolates in Two Chilean Cities

This paper describes attempts to isolate and characterize glycosaminoglycan (GAG)-binding molecules on the surface of lymphocytes and lymphoma cell lines and relate their expression to splenic and lymph...Full Text Available

1991-02-01

211

CASPAR: Low-Cost, Dual-Manifest Payload Adapter for ...

We compared the incidence of nasopharyngeal colonization by Streptococcus pneumoniae, the serotypes causing mucosal and invasive diseases, and the antibiotic resistance of these strains...Full Text Available

1998-03-01

212

Biology of Pseudomonas stutzeri

... being designed for projected Minotaur IV launch load environments, with design objectives of light weight, integrated vibration isolation, low shock ...

2011-05-14

213

BAM: Diarrheagenic Escherichia coli

Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the...Full Text Available

2006-06-01

214

Antimicrobial Susceptibility of Haemophilus ducreyi

... Confirm isolates that appear as typical E. coli on L-EMB and are X-gal (+), MUG (+) or (-) and ... Hill, WE, KC Jinneman, PA Trost, JL Bryant, J. Bond and MM Wekell ...

215

Antimicrobial Ointments and Methicillin-Resistant Staphylococcus...

The susceptibility of 19 isolates of Haemophilus ducreyi from a recent chancroid outbreak and four reference strains was determined in vitro to 13 antimicrobial agents. The rabbit intradermal...Full Text Available

1978-04-01

216

Ancient Pollen Yields Insight into Forest Biodiversity

Staphylococcus aureus isolates and 2 USA300 ATCC type strains for susceptibility to bacitracin and neomycin contained in over-the-counter antibacterial ointments. Resistance to...

2011-10-15

217

An Input to the UVOIR Panel Of the AASC April ... - PlanetQuest - NASA

... data on pollen from red maple, birch, beech, ash, oak, hemlock and elm trees isolated from cores of ...

218

A search for the epidemic typhus agent in Ethiopian ticks*

Binary Star Evolution. The evolution of close binary stars can be very different from that of wide binaries of isolated stars. If the stars are close enough ...

219

9902631 - Lysozyme - NASA

The presence of antibodies to Rickettsia prowazeki in domestic animals from several parts of Africa, and the isolation of this rickettsia from the blood of goats and sheep and from...Full Text Available

1973-01-01

220

Widespread Dissemination of Aminoglycoside Resistance Genes armA and rmtB in Klebsiella pneumoniae Isolates in Taiwan Producing CTX-M-Type Extended-Spectrum ?-Lactamases ?

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

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221

The metabolism of L-tryptophan by isolated rat liver cells. Effect of albumin binding and amino acid competition on oxidatin of tryptophan by tryptophan 2,3-dioxygenase.

Among 235 extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL) isolates collected from a nationwide surveillance performed in Taiwan, 102 (43.4%) were...Full Text Available

2009-01-01

222

Radioautographic Analysis of the Distribution of Label from ³H-Indoleacetic Acid Supplied to Isolated Coleus Internodes ¹

1. Novel methods, using L-[ring-2-14C]tryptophan, are described for the measurement of tryptophan 2,3-dioxygenase activity and tryptophan accumulation in isolated rat liver cells. 2. The effects of...Full Text Available

1980-03-15

223

Optical voltage reference

Isolated fifth internodes of Coleus blumei Benth. were supplied with ³H-IAA at their apical ends. Microradioautography, using thin sections (0.25-0.5 microns) of Epon embedded...Full Text Available

1969-01-01

224

New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity.

An optical voltage reference for providing an alternative to a battery source. The optical reference apparatus provides a temperature stable, high precision, isolated voltage reference through the use of optical isolation techniques to eliminate current and impedance coupling errors. Pulse rate frequency modulation is employed to eliminate errors in the optical transmission link while phase-lock feedback is employed to stabilize the frequency to voltage transfer function.

1992-12-31

225

New Variant of CTX-M-Type Extended-Spectrum ?-Lactamases, CTX-M-71, with a Gly238Cys Substitution in a Klebsiella pneumoniae Isolate from Bulgaria?

Seventeen clinical isolates of Staphylococcus aureus (from the United States and Europe) selected for low (borderline)-level methicillin resistance (MIC of methicillin, 2 to 4 micrograms/ml; MIC of...Full Text Available

1989-11-01

226

Nationwide Survey of CTX-M-Type Extended-Spectrum ?-Lactamases among Klebsiella pneumoniae Isolates in Slovenian Hospitals ?

A single Klebsiella pneumoniae strain isolated in a Bulgarian hospital was found to produce CTX-M-71, a new CTX-M variant characterized by one amino acid substitution...Full Text Available

2009-10-01

227

Molecular Characterization of Vibrio cholerae O139 Bengal Isolated from Water and the Aquatic Plant Eichhornia crassipes in the River Ganga, Varanasi, India

Among 177 extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates collected from 11 Slovenian hospitals in 2005 and 2006, 60 (34%), from eight hospitals,...Full Text Available

2009-01-01

228

Korea-Japan joint research on development of seismic capacity evaluation and enhancement technology considering near-fault effect (annual report 2004)

A collection of ten strains of Vibrio cholerae O139, comprising six isolates from Eichhornia crassipes, two from water of the River Ganga, and one each from a well...Full Text Available

2003-04-01

229

Isolation of a bacterial host selective for bacteriophage T4 containing cytosine in its DNA.

In Chapter 2 the evaluation methods of input ground motions for seismic design are summarized and especially the empirical and/or stochastic Green's function method is introduced in order to calculate the broadband strong motions based on the physically reliable fault model. Then the evaluation procedure is applied to the Ulsan fault system near which Wolsung nuclear power plant is located. In chapter3, the PSHA case studies for Wolsung NPP site are performed, in which multi attenuation equations of earthquake motions are adopted. Moreover, the results of seismic hazard evaluation for eight sites in Korea are shown. In addition, the contents of two literatures on seismic hazard evaluation are introduced. In chapter 4, isolation devices for emergency diesel generator have been studied. The models for friction-pendulum system, high damping rubber bearing and natural rubber bearing are developed and implemented in the TDAP-III. Then, the rubber bearings as ...

2004-12-01

230

Isolation and characterization of cDNA clones for human skeletal muscle alpha actin.

An Escherichia coli B strain, B834 galU56, has been isolated which supports growth of bacteriophage T4 with cytosine in its DNA while restricting growth of T4 with hydroxymethylcytosine. This host is...Full Text Available

1978-09-01

231

Isolation and Initial Characterization of Constitutive Nitrate Reductase-Deficient Mutants NR328 and NR345 of Soybean (Glycine max) ¹

Two cDNA libraries corresponding to polyA+ RNA from human adult skeletal muscle have been constructed by cloning in the PstI site of pBR322. Skeletal alpha actin cDNA clones have been isolated and characterized....Full Text Available

1983-06-11

232

In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I

Two nitrate reductase deficient mutants of soybean (Glycine max [L.] Merr. cv Bragg) were isolated from approximately 10,000 M₂ seedlings, using a direct enzymic assay in...Full Text Available

1986-06-01

233

High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

We previously showed that very thin filamentous bacteria affiliated with the division green non-sulfur bacteria were abundant in the outermost layer of thermophilic methanogenic sludge granules fed...Full Text Available

2001-12-01

234

Genetic and Structural Insights into the Dissemination Potential of the Extremely Broad-Spectrum Class A ?-Lactamase KPC-2 Identified in an Escherichia coli Strain and an Enterobacter cloacae Strain Isolated from the Same Patient in France?

A total of 204 isoniazid (INH)-resistant strains of Mycobacterium tuberculosis isolated from different patients in the northwestern region of Russia from 1996 to 2001 were screened...Full Text Available

2002-05-01

235

Extended-Spectrum-?-Lactamase-Producing Escherichia coli Strains Isolated from Farm Animals from 1999 to 2002: Report from the Japanese Veterinary Antimicrobial Resistance Monitoring Program

Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum...Full Text Available

2008-10-01

236

Chromosomal Integration of the Extended-Spectrum ?-Lactamase Gene bla_CTX-M-15 in Salmonella enterica Serotype Concord Isolates from Internationally Adopted Children?

A nationwide surveillance for antimicrobial susceptibility in Escherichia coli strains isolated from food-producing animals in Japan was conducted from 1999 to 2002. Eighteen cefazolin-resistant...Full Text Available

2005-08-01

237

Characterization of Fatty Acid Biosynthesis in Isolated Pea Root Plastids ¹

We report the emergence of Salmonella enterica isolates of serotype Concord (and its monophasic variant 6,7:l,v:-) producing the extended-spectrum β-lactamases (ESBLs) SHV-12...Full Text Available

2009-05-01

238

Characterization of CTX-M ESBLs in Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae clinical isolates from Cairo, Egypt

Fatty acid biosynthesis from Na[1-¹⁴C]acetate was characterized in plastids isolated from primary roots of 7-day-old germinating pea (Pisum sativum L.) seeds. Fatty acid...Full Text Available

1991-06-01

239

CTX-M-14, a Plasmid-Mediated CTX-M Type Extended-Spectrum ?-Lactamase Isolated from Escherichia coli

BackgroundA high rate of resistance to 3^rdgeneration cephalosporins among Enterobacteriaceae isolates from Egypt has been previously reported. This study aims to characterize...Full Text Available

240

Antimicrobial resistance, virulence-associated genes, and pulsed-field gel electrophoresis profiles of Salmonella enterica subsp. enterica serovar Typhimurium isolated from piglets with diarrhea in Korea

Four Escherichia coli isolates harboring CTX-M-14, with a single Ala231→Val substitution compared to CTX-M-9, had three different ribotypes. Cefotaxime resistance was plasmid...Full Text Available

2002-06-01

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241

Use of DNA probes to study tetracycline resistance determinants in gram-negative bacteria from swine

Salmonella enterica subsp. enterica serovar Typhimurium was isolated from diarrheic piglets in 2 periods, 2000–2001 (n = 25) and 2005–2006...Full Text Available

2011-01-01

242

Comparison of secondary metabolite production by Penicillium crustosum strains, isolated from Arctic and other various ecological niches.

Specific {sup 32}P-labeled DNA probes were prepared and used to evaluate the distribution of tetracycline resistance determinants carried by gram-negative enteric bacteria isolated from pigs in 3 swine herds with different histories of antibiotic exposure. Plasmid DNA, ranging in size from 2.1 to 186 Kb, was observed in over 84% of 114 isolates studied. Two of 78 tetracycline resistant strains did not harbor plasmids. The DNA probes were isolated from plasmids pSL18, pRT29/Tn10, pBR322 and pSL106, respectively, and they represented class A, B, C and D tetracycline resistance determinants. Hybridization conditions using 0.5X SSPE at 65{degrees}C minimize cross-hybridization between the different class of tetracycline resistance genes. Cross-hybridization between class A and class C determinants could be distinguished by simultaneous comparison of the intensity of their hybridization signals. Plasmids from over 44% of the ...

1989-01-01

243

ent-Rosane and abietane diterpenoids as cancer chemopreventive agents

Penicillium crustosum is common in food and feed both in subtropical and temperate regions. Recently, it has also been found occurring frequently in glacier ice, sea ice and sea water of Arctic regions of Svalbard. The aim of the study was to compare isolates of the same fungal species from widely different habitats and geographic regions to see if the nutritional physiology and the profile of secondary metabolites were consistent or depended on the isolation source. All 121 strains examined produced the following families of secondary metabolites: penitrems (100%), roquefortines (100%), terrestric acids (99.2%) and viridicatols (100%), whereas 81 of 83 Arctic isolates additionally produced andrastin A. However, only 8 of 38 non-Arctic isolates produced detectable andrastin A. The quantitative profiles of 96 strains were compared using cluster, principal component and correspondence analyses. There was ...

2004-12-22

244

British Library Electronic Table of Contents (United Kingdom)

Two ent-rosane- (cuzcol, 1 and 6-dehydroxycuzcol, 2) and a abietatriene- (salvadoriol, 3) type diterpenoids have been isolated from Maytenus cuzcoina and Crossopetalum uragoga, respectively, along with five known diterpene compounds (4-8). Their stereostructures have been elucidated on the basis of spectroscopic analysis, including 1D and 2D NMR techniques, and computational data. The absolute configuration of cuzcol was determined by application of Riguera ester procedure. This is the first instance of isolation of ent-rosane diterpenoids from species of the Celastraceae. The isolated diterpenes were found to be potent anti-tumour-promoter agents, and carnosol (7) also showed a remarkable chemopreventive effect in an in vivo two-stage carcinogenesis model.

2011-01-01

245

Use of a trap garden to find additional genetically distinct isolates of the rust fungus Phragmidium violaceum to enhance biological control of European blackberry in Australia

British Library Electronic Table of Contents (United Kingdom)

Biological control agents can be more effective if their populations are genetically diverse, particularly when the target invasive plant comprises a range of genotypes with different susceptibilities and occurs across various microclimates. We report on the use of an efficient approach to find, in the native range, diverse isolates of a rust fungus for biological control. An outdoor trap garden containing various clones of invasive European blackberry (Rubus fruticosus agg.) collected in Australia, each with a different DNA phenotype, was established in France. Within 4?weeks of establishment, the leaf-rust fungus Phragmidium violaceum was recovered from all clones in the garden. Molecular analyses of eight recovered and purified isolates of the fungus from the garden revealed that they w...

2011-01-01

246

Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran

British Library Electronic Table of Contents (United Kingdom)

This study investigated the occurrence of free-living amoebae (FLA) in immunodeficiency wards of hospitals in Tehran, Iran. A total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), HIV, leukemia and oncology patients of five university hospitals were collected and examined for the presence of FLA using culturing and molecular approaches. Based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of Acanthamoeba, vahlkampfiid amoebae and Hartmannella. Out of 70 samples, 37 (52.9%) were positive for FLA. Acanthamoeba belonged to the T4 genotype was the most prevalent isolate. Presence of the T4 genotype on medical instruments, including an oxygen mask in an isolation room of an immunodeficiency pediat...

2011-01-01

247

Network Security Alerts Management Architecture for Signature-Based Intrusions Detection Systems within a NAT Environment

British Library Electronic Table of Contents (United Kingdom)

Internet is providing essential communication between an infinite number of people and is being increasingly used as a tool for commerce. At the same time, security is becoming a tremendously important issue to deal with. Different network security solutions exist and contribute to enhanced security. From these solutions, Intrusion detection systems (IDS) have become one of the most common countermeasures for monitoring safety in computer systems and networks. The purpose of IDSs is distinguishing between intruders and normal users. However, IDSs report a massive number of isolated alerts. These isolated alerts represent low-level security-related events. Many of these isolated alerts are logically involved in a single multi-stage intrusion incident and a security officer often wants to an...

2011-01-01

248

Myocardial pharmacokinetics of ebastine, a substrate for cytochrome P450 2J, in rat isolated heart

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND AND PURPOSE It is well established that cytochrome P450 2J (CYP2J) enzymes are expressed preferentially in the heart, and that ebastine is a substrate for CYP2J, but it is not known whether ebastine is metabolized in myocardium. Therefore, we investigated its pharmacokinetics in the rat isolated perfused heart. EXPERIMENTAL APPROACH Rat isolated hearts were perfused in the recirculating mode with ebastine for 130-min. The concentrations of ebastine and its metabolites, hydroxyebastine and carebastine, were measured using liquid chromatography with a tandem mass spectrometry. The data were analysed by a compartmental model. The time course of negative inotropic response was linked to ebastine concentration to determine the concentration-effect relationship. KEY RESULTS Ebastine w...

2011-01-01

249

MCNP study for epithermal neutron irradiation of an isolated liver at the Finnish BNCT facility

Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization

A successful boron neutron capture treatment (BNCT) of a patient with multiple liver metastases has been first given in Italy, by placing the removed organ into the thermal neutron column of the Triga research reactor of the University of Pavia. In Finland, FiR 1 Triga reactor with an epithermal neutron beam well suited for BNCT has been extensively used to irradiate patients with brain tumors such as glioblastoma and recently also head and neck tumors. In this work we have studied by MCNP Monte Carlo simulations, whether it would be beneficial to treat an isolated liver with epithermal neutrons instead of thermal ones. The results show, that the epithermal field penetrates deeper into the liver and creates a build-up distribution of the boron dose. Our results strongly encourage further studying of irradiation arrangement of an isolated liver with epithermal neutron fields.

2004-11-01

250

British Library Electronic Table of Contents (United Kingdom)

Isolation of functional and intact mitochondria from solid tissue is crucial for studies that focus on the elucidation of normal mitochondrial physiology and/or mitochondrial dysfunction in conditions such as aging, diabetes, and cancer. There is growing recognition of the importance of mitochondria both as targets for drug development and as off-target mediators of drug side effects. Unfortunately, mitochondrial isolation from tissue is generally carried out using homogenizer-based methods that require extensive operator experience to obtain reproducible high-quality preparations. These methods limit dissemination, impede scale-up, and contribute to difficulties in reproducing experimental results over time and across laboratories. Here we describe semiautomated methods to disrupt tissue ...

2011-01-01

251

Fungi of the house dust in Riyadh, Saudi Arabia.

Fourier transform IR studies on the interaction of selected chemicals with isolated cuticles

30 species belonging to 18 genera were isolated from floor dust of 30 homes in Riyadh. Out of them 16 species and 10 genera were isolated from dust of air conditioners of the same homes. The most common genera in floor dust were Aspergillus, Penicillium and Cladosporium. Aspergillus repens, A. amstelodami, A. versicolor, A. fumigatus, Penicillium purpurogenum, P. crustosum, Cladosporium cladosporoides and C. herbarum were frequently isolated. The most abundant genera in air conditioner dust were Aspergillus and Penicillium. Aspergillus fumigatus, A. ochraceus, A. terreus, Penicillium oxalicium and P. crustosum were most frequent species. PMID:2330766

1990-01-01

252

Five naturally bioactive molecules including two rhamnopyranoside derivatives isolated from the Streptomyces sp. strain TN58

It is known that the plant cuticle represents the first barrier that must be overcome by any chemical reaching the plant surface from the atmosphere before entering the plant. Because of the importance of the cuticle as a barrier to penetration of a wide variety of compounds, its morphology, chemistry, and permeability have been extensively studied. However, only limited information is available on the nature of functional chemical groups present and their interaction and role during the penetration process. The usefulness of in situ Fourier transform infrared spectroscopy studies in identifying functional groups present in isolated cuticles is described and their relationships to the structure of the cuticular membrane are discussed. Applications of infrared spectroscopy on the presence and role of phenolics in the cuticle structure and during the cuticle development, nitrogen oxide binding to isolated cuticles, and the interactions between ...

253

British Library Electronic Table of Contents (United Kingdom)

Extraction of 25 L fermentation broth of the newly isolated Streptomyces sp. strain TN58 and various separation and purification steps led to the isolation of five bioactive metabolites, namely brevianamide F (C1), reported from a streptomycete for the first time, N?-acetyltryptamine (C2), thiazolidomycin (C3), and two rhamnopyranosides (C4 and C5). These two rhamnopyranosides were produced directly, without precursor addition. The chemical structure of these five active compounds was established on the basis of 1H, 13C/APT and 2D NMR spectra, ESI and EI-MS data, and by comparison with data from the literature. According to the biological studies, we show in this work that the compounds C1, C2, C4 and C5 possess antimicrobial activities.

2009-01-01

254

Dielectric-wall linear accelerator with a high voltage fast rise time switch that includes a pair of electrodes between which are laminated alternating layers of isolated conductors and insulators

Dielectric-wall linear accelerator with a high voltage fast rise time switch that includes a pair of electrodes between which are laminated alternating layers of isolated conductors and insulators

A dielectric-wall linear accelerator is improved by a high-voltage, fast rise-time switch that includes a pair of electrodes between which are laminated alternating layers of isolated conductors and insulators. A high voltage is placed between the electrodes sufficient to stress the voltage breakdown of the insulator on command. A light trigger, such as a laser, is focused along at least one line along the edge surface of the laminated alternating layers of isolated conductors and insulators extending between the electrodes. The laser is energized to initiate a surface breakdown by a fluence of photons, thus causing the electrical switch to close very promptly. Such insulators and lasers are incorporated in a dielectric wall linear accelerator with Blumlein modules, and phasing is controlled by adjusting the length of fiber optic cables that carry the laser light to the insulator surface.

1998-01-01

255

Design and control of six degree-of-freedom active vibration isolation table.

A dielectric-wall linear accelerator is improved by a high-voltage, fast rise-time switch that includes a pair of electrodes between which are laminated alternating layers of isolated conductors and insulators. A high voltage is placed between the electrodes sufficient to stress the voltage breakdown of the insulator on command. A light trigger, such as a laser, is focused along at least one line along the edge surface of the laminated alternating layers of isolated conductors and insulators extending between the electrodes. The laser is energized to initiate a surface breakdown by a fluence of photons, thus causing the electrical switch to close very promptly. Such insulators and lasers are incorporated in a dielectric wall linear accelerator with Blumlein modules, and phasing is controlled by adjusting the length of fiber optic cables that carry the laser light to the insulator surface. 12 figs.

1998-10-13

256

Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria

A six-axis active vibration isolation system (AVIS) is designed by using the direct driven guide and ball contact mechanisms in order to have no cross-coupling between actuators. The point contact configuration gives an advantage of having an easy assembly of eight voice coil actuators to an upper and a base plate. A voice coil actuator is used since it can provide a large displacement and sufficient bandwidth required for vibration control. The AVIS is controlled considering the effect of flexible vibration mode in the upper plate and velocity sensor dynamics. A loop shaping technique and phase margin condition are applied to design a vibration controller. The performances of the AVIS are investigated in the frequency domain and finally validated by comparing with the passive isolation system. The scanning profiles of the specimen are compared together by using the atomic force microscope. The robustness of the AVIS is verified by showing the ...

2010-03-01

257

British Library Electronic Table of Contents (United Kingdom)

Studies were carried out on the decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Among the 27 strains of halophilic and halotolerant bacteria isolated from effluents of textile industries, three showed remarkable ability in decolorizing the widely utilized azo dyes. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that these strains belonged to the genus Halomonas. The three strains were able to decolorize azo dyes in a wide range of NaCl concentration (up to 20%w/v), temperature (25-40degreeC), and pH (5-11) after 4 days of incubation in static culture. They could decolorize the mixture of dyes as well as pure dyes. These strains also readily grew in and decolorized the high concentrations of dy...

2007-01-01

258

Comparative study of functional properties of commercial and membrane processed yellow pea protein isolates

British Library Electronic Table of Contents (United Kingdom)

Functional properties of commercial and membrane processed pea protein isolates (PPI) prepared from yellow peas were investigated. Four protein isolates were prepared from yellow pea flour using water and KCl extractions at 25^oC followed by ultrafiltration and diafiltration (UF and DF) at pHs of 7.5 and 7.5 or 6 respectively. Following assessment of compositional attributes; solubility, foaming, flow and dynamic rheology, emulsification ability and heat-induced textural and rheological properties of prepared PPIs and a commercially available PPI were tested and compared. Membrane purification of proteins resulted in 28% to 68% reduction in phytic acid and enhanced, comparatively, the tested functional properties. Solubility of membrane processed PPIs, at all tested pHs, was superior and t...

2011-01-01

259

Charging a Battery-Powered Device with a Fiber-Optically Connected Photonic Power System for Achieving High-Voltage Isolation

Agni’s fungi: heat-resistant spores from the Western Ghats, southern India

This paper describes the development and testing of a system to provide isolated power to the cathode-subsystem electronics of an x-ray tube. These components are located at the cathode potential of several hundred kilovolts, requiring a supply of power isolated from this high voltage. In this design a fiber-optically connected photonic power system (PPS) is used to recharge a lithium-ion battery pack, which will subsequently supply power to the cathode-subsystem electronics. The suitability of the commercially available JDSU PPS for this application is evaluated. The output of the ppe converter is characterized. The technical aspects of its use for charging a variety of Li-Ion batteries are discussed. Battery charge protection requirements and safety concerns are also addressed.

2008-01-01

260

British Library Electronic Table of Contents (United Kingdom)

This study concerns the thermotolerance of spores of mesophilic fungi isolated from a tropical semi-arid habitat subject to dry season fire in the Western Ghats, southern India. Among 25 species of Ascomycota isolated from leaf litter, nine were able to grow after incubation in a drying oven for 2h at 100degreeC; the spores of two of these species survived 2h incubation at 110degreeC, and one survived exposure to 115degreeC for 2h. The range of thermotolerance among mesophilic fungi isolated from the leaf litter was surprising: filamentous fungi from other habitats, including species that colonize scorched vegetation after fires and thermophilic forms occurring in self-heating plant composts, cannot survive even brief exposure to such high temperatures. It is possible that the exceptional ...

2011-01-01

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261

Aerobic Decolorization and Detoxification of a Disperse Dye in Textile Effluent by a New Isolate of Bacillus sp.

British Library Electronic Table of Contents (United Kingdom)

A number of aerobic species capable of decolorizing some of the dyes in a textile mill effluent were isolated. One of the isolates was able to decolorize Terasil black dye under aerobic conditions in the presence of an exogenous carbon source after 5 days. Glucose or starch (%1 ea) are essential for decolorization but the process proceeds faster in the presence of 0.5% yeast extract. Results of the BOD5 show that the untreated effluent samples have a low BOD value, whereas treated samples show an initial increase in BOD up to 15 days followed by a decrease after 20 days. FT-IR and GC-MS data also reveal that the initial components in the untreated effluent disappear after 20 days of treatment, confirming biodegradation of the dye. Phytotoxicity tests on the untreated effluent samples using...

2006-01-01

262

Survival Estimates for the Passage of Juvenile Salmonids through Snake River Dams and Reservoirs, 1996 Annual Report

Soil chemistry and nutrition of North American spruce-fir stands: Evidence of recent change

In 1996, the National Marine Fisheries Service and the University of Washington completed the fourth year of a multi-year study to estimate survival of juvenile salmonids (Oncorhynchus spp.) passing through dams and reservoirs on the Snake River. Actively migrating smolts were collected near the head of Lower Granite Reservoir and at Lower Granite Dam, tagged with passive integrated transponder (PIT) tags, and released to continue their downstream migration. Individual smolts were subsequently detected at PIT-tag detection facilities at Lower Granite, Little Goose, Lower Monumental, McNary, John Day and Bonneville Dams. Survival estimates were calculated using the Single-Release (SR) and Paired-Release (PR) Models. Timing of releases of tagged hatchery steelhead (O. mykiss) from the head of Lower Granite Reservoir and yearling chinook salmon (O. tshawytscha) from Lower Granite Dam in 1996 spanned the major portion of their juvenile migrations. Specific research ...

1998-02-01

263

Repowering: improving the economics of existing assets

One set of hypotheses offered to explain the decline of red spruce (Picea rubens Sarg.) in eastern North America focuses on the effect of acidic deposition on soil chemistry changes that may affect nutrient availability and root function. Long-term soils data suggests that soil acidification has occurred in some spruce stands over the past 50 yr, with plant uptake and cation leaching both contributing to the loss of cations. Studies of tree ring chemistry also have indicated changes in Ca/Al and Mg/Al ratios in red spruce wood, suggesting increases in the ionic strength of soil solution. Irrigation studies using strong acid inputs have demonstrated accelerated displacement of base cations from upper horizons. Spruce-fir (Abies spp.) nutrient budgets indicate that current net Ca and Mg leaching loss rates are of the same order of magnitude as losses to whole tree harvest removals, spread out over a 50-yr rotation. For most cations, red spruce foliar nutrient levels ...

264

Impact of heavy metals on macro-invertebrate fauna of the thaddo stream

Restructured power markets require concepts not only for extending the lifetime of existing plants, but also concepts for reducing the life-cycle costs of existing older steam power plants in order to remain competitive with the most modern and advanced power generation technology such as combined cycle power plants. Out of the broad range of methods to extend service life and increase availability, and to increase power output and efficiency, this paper focuses on full repowering. Full repowering replaces the old fired boiler with gas turbine(s) and heat-recovery steam generators (HRSG) as heat input to the existing bottoming cycle and reuses the steam turbine (ST) condenser and further balance of plant (BOP) equipment. The potential offered by this concept is demonstrated. Repowering and modernization projects are executed if they result in economic benefits for the operator. Investment decisions need to be prepared based on an estimate or proof of an adequate return on investment ...

2005-07-01

265

Effect of temperature on the development of Steinernema carpocapsae and Steinernema feltiae (Nematoda: Rhabditida) in liquid culture.

Impact of some heavy metals like zinc, lead, copper, chromium and cadmium were studied at four spots on the macro-invertebrate fauna of the Thaddo stream, a tributary of Malir River. This was in correlation with an earlier study on the physico-chemical aspects of water which showed a severe pollution in this stream. Present data for the qualitative and quantitative analyses of macro-invertebrates and the ranges of heavy metals (Zn 0.5-3.5, Pb 0.90-1.42, Cu 0.35-0.93, Cr 0.0-0.08 and Cd 0.003-0.01 ppm) in the water samples also indicate high level of pollution in the stream. Macro-invertebrate fauna comprises only of aquatic insects which include larvae of Chironomus spp., adults of the Notonectus sp., and nymphs of Gomphus sp. (dragon fly) belonging to the order Diptera , Hemiptera and Odonata, respectively. Quantitatively Notonectus sp. predominated and followed by Chironomus larvae. The maximum concentrations of all heavy metals were recorded at spot 3. A general ...

266

Ecological evaluation of proposed dredged material from Richmond Harbor Deepening Project and the intensive study of the Turning Basin

For commercial use of the entomopathogenic nematodes Steinernema carpocapsae and Steinernema feltiae in biological control of insect pests, they are produced in liquid culture on artificial media pre-incubated with their symbiotic bacteria Xenorhabdus nematophila and Xenorhabdus bovienii, respectively. After 1 day of the bacterial culture, nematode dauer juveniles (DJs) are inoculated, which recover development. The adult nematodes produce DJ offspring, which are harvested and can be sprayed. This study determined optimal temperatures to obtain high DJ progeny within a short process time. Temperatures assessed were 23 degrees C, 25 degrees C, 27 degrees C, and 29 degrees C for S. carpocapsae and 20 degrees C, 23 degrees C, 25 degrees C, and 27 degrees C for S. feltiae. The recovery of inoculated DJs was hardly affected and was reduced only in S. carpocapsae at 29 degrees C. The fecundity (eggs in uterus) in S. carpocapsae reached a maximum at 27 degrees C; whereas, maximum yields were ...

2009-05-20

267

Changes in the behaviour and physical and chemical characteristics of soil after adding populus euramericana leaves

Richmond Harbor is on the eastern shoreline of central San Francisco Bay and its access channels and several of the shipping berths are no longer wide or deep enough to accommodate modem deeper-draft vessels. The Water Resources Development Act of 1986 (PL99-662) authorized the US Army Corps of Engineers (USACE), San Francisco District to deepen and widen the navigation channels in Richmond Harbor. Several options for disposal of the material from this dredging project are under consideration by USACE: disposal within San Francisco Bay, at open-ocean disposal sites, or at uplands disposal sites. Purpose of this study was to conduct comprehensive evaluations, including chemical, biological, and bioaccumulation testing of sediments in selected areas of Richmond Harbor. This information was required by the Environmental Protection Agency (EPA) and USACE. Battelle/Marine Sciences Laboratory collected 20 core samples, both 4-in. and 12-in., to a project depth of -40 ft mean lower low water ...

1995-06-01

268

Use of synthetic oligonucleotide DNA probes for identification and direct detection of Bacteroides forsythus in plaque samples.

Soil erosion and small annual additions of organic matter from plant-sources are the major causes of low organic-matter content in our soils. The tops of the plants, fallen to the soil- surface, remain there are incorporated, the plant-roots, shrubs, grasses. And other native plants contribution much towards the soil organic matter. Populus spp. Are grown commonly around farmers' fields in the state of Azad Jammu and Kashmir. A pot-experiment was conducted to study the effect of addition of populus euramericana leaves on various physical and chemical characteristics of the soil. Soil was kept at field-capacity level and incubated at room temperature for 10 months after adding 25, 50, and 75 g of Populus curamericana leaves per pot. Changes in organic-matter content. PH, cation-exchange capacity extractable potassium, water-holding capacity, and bulk density were investigated, after incubation for 6,8,and 10 months. There was a linear increase in organic-matter ...

269

Triosephosphates Modulate Leaf Mitochondrial Phosphorylation by Inhibition and Uncoupling of Electron Transport ¹

Oligonucleotide DNA probes complementary to the hypervariable region of the 16S rRNA of Bacteroides forsythus were tested for their specificity and sensitivity against reference and clinical isolates...Full Text Available

1991-10-01

270

Transport and deamination of amino acids by a gram-positive, monensin-sensitive ruminal bacterium.

The effect of TP (triosephosphates:glyceraldehyde-3 phosphate, GAP, +dihydroxyacetone phosphate, DHAP) on respiration, phosphorylation and matrix ATP/ADP ratios of isolated oat mesophyll mitochondria...Full Text Available

1985-11-01

271

Transmural distribution of extracellular purines in isolated guinea pig heart.

Strain F, a recently isolated ruminal bacterium, grew rapidly with glutamate or glutamine as an energy source in the presence but not the absence of Na. Monensin, a Na+/H+ antiporter, completely inhibited...Full Text Available

1990-07-01

272

Transmission of Salmonella mbandaka to cattle from contaminated feed.

The purine adenosine appears to be involved in regulation of coronary vascular tone. Little is known concerning the levels and distribution of adenosine and related purines in the extracellular fluid...Full Text Available

1991-01-15

273

Transduction noise induced by 4-hydroxy retinals in rod photoreceptors.

Salmonella mbandaka was isolated from cattle on three dairy farms. The duration of infection was less than four weeks and none of the animals became clinically ill. The animals had all consumed a diet...Full Text Available

1982-04-01

274

Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1

New visual pigments were formed with 4-hydroxy retinals in isolated vertebrate rod photoreceptors by exposing bleached rods from the tiger salamander, Ambystoma tigrinum, to lipid vesicles containing...Full Text Available

1990-01-01

275

The reactivity of thiol groups and the subunit structure of aldolase

Known vertebrate GATA proteins contain two zinc fingers and are required in development, whereas invertebrates express a class of essential proteins containing one GATA-type zinc finger. We isolated...Full Text Available

2001-04-02

276

The prosthetic group of methanol dehydrogenase. Purification and some of its properties.

1. Seven unique carboxymethylcysteine-containing peptides have been isolated from tryptic digests of rabbit muscle aldolase carboxymethylated with iodo[2-¹⁴C]acetic acid in 8m-urea....Full Text Available

1970-04-01

277

The influence of mucus on the diffusion of chloride ions across the oesophagus of the minnow (Phoxinus phoxinus (L.].

Methanol dehydrogenases isolated from bacteria belonging to different classes of methylotrophs contain the same prosthetic group. A procedure for its purification from whole cells is given. The reduced...Full Text Available

1980-04-01

278

The effect of swimming exercise on spontaneous ventricular defibrillation and ventricular fibrillation threshold in the isolated perfused rat heart.

Micro-electrodes sensitive to Cl- have been used to measure Cl- activities in the mucous layer on the mucosal surface of an isolated oesophageal preparation of the fresh-water teleost, Phoxinus phoxinus...Full Text Available

1984-01-01

279

The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008

Effects of swimming exercise training on left ventricular contraction and relaxation, the incidence of spontaneous defibrillation and the ventricular fibrillation threshold were studied in the isolated, perfused rat heart. The heart/body weight ratio was 4.8 +/- 0.4 x 10(-3) in swimmers compared with 3.9 +/- 0.6 x 10(-3) in sedentary controls (p less than 0.0005). The spontaneous heart rate was lower in isolated hearts of trained animals (222 +/- 18 bpm compared with 244 +/- 28, p less than 0.05). Left ventricular systolic pressure was higher in trained hearts and the rate of ventricular relaxation enhanced (p less than 0.05). The ventricular fibrillation threshold was 7.2 +/- 5.2 and 10.8 +/- 6.1 mamp in hearts of swimmers and controls, respectively, the difference not being significant. The incidence of spontaneous defibrillation was not significantly different between the two groups. It is concluded that, while swimming induces cardiac ...

1990-12-01

280

Spread of a Salmonella typhimurium Clone Resistant to Expanded-Spectrum Cephalosporins in Three European Countries

BackgroundStreptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial...Full Text Available

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281

Some characteristics of a secreted chlamydial antigen recognized by IgG from C. trachomatis patient sera.

Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance...Full Text Available

1999-11-01

282

Serratia odorifera biogroup 1 causing an invasive human infection.

Chlamydia trachomatis serovars release a glycolipid antigen (GLXA) into the culture supernatant during the infective cycle. This antigen is recognized by IgG isolated from humans with a natural chlamydial...Full Text Available

1989-12-01

283

Salmonellosis in Botswana

Serratia odorifera biogroup 1 was isolated from the blood and urine of an alcoholic male with cirrhosis and signs of septic shock. The organism is rarely reported to occur in clinical specimens. This...Full Text Available

1988-06-01

284

Role of the nac gene product in the nitrogen regulation of some NTR-regulated operons of Klebsiella aerogenes.

Clinical salmonellosis in cattle is infrequently recorded in Botswana, the majority of salmonella isolations being made from apparently healthy animals. A salmonella carrier rate of 6·7% was...Full Text Available

1971-09-01

285

Role of copper resistance in competitive survival of Pseudomonas fluorescens in soil.

A positive, genetic selection against the activity of the nitrogen regulatory (NTR) system was used to isolate insertion mutations affecting nitrogen regulation in Klebsiella aerogenes. Two classes...Full Text Available

1990-12-01

286

Role of Calcitonin Gene-Related Peptide in Bone Repair after Cyclic Fatigue Loading

A copper-resistant strain (09906) of Pseudomonas fluorescens that was isolated from a citrus grove soil is being investigated as a biological control agent for Phytophthora root rot. Since citrus grove...Full Text Available

1993-02-01

287

Requirements for Extraction of Polyribosomes from Plant Callus Cultures ¹

BackgroundCalcitonin gene related peptide (CGRP) is a neuropeptide that is abundant in the sensory neurons which innervate bone. The effects of CGRP on isolated bone cells have been...Full Text Available

288

Relative potencies for barbiturate binding to the Torpedo acetylcholine receptor.

A procedure was developed to isolate polyribosomes from plant cell cultures. Tobacco callus extracted in 10 mm MgCl₂, 80 mm KCl, 250 mm sucrose, and 140 mm...Full Text Available

1977-05-01

289

Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae.

1. The structural requirements of an allosteric barbiturate binding site on acetylcholine receptor-rich membranes isolated from Torpedo electroplaques have been characterized by the ability of fourteen...Full Text Available

1990-11-01

290

Regulation of Vacuolar H⁺-Pyrophosphatase by Free Calcium ¹

The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile...Full Text Available

1993-08-01

291

Regulation of Calcium Influx in Chara¹

The H⁺-translocating inorganic pyrophosphatase (H⁺-PPase) associated with vesicles of the vacuolar membrane (tonoplast) isolated from beet (Beta vulgaris L.) is...Full Text Available

1992-12-01

292

Reduced PCR Sensitivity Due to Impaired DNA Recovery with the MagNA Pure LC Total Nucleic Acid Isolation Kit

Measurements were made of ⁴⁵Ca influx into isolated internodal cells of Chara corallina and also into internodal cells of intact plants. ⁴⁵Ca influx was closely...Full Text Available

1992-10-01

293

Recovery of Pathogenic Measles Virus from Cloned cDNA

The increasing demand for molecular diagnostics in clinical microbiology laboratories necessitates automated sample processing. In the present study, we evaluated the performance of the MagNA Pure LC...Full Text Available

2005-09-01

294

Rapid isolation and identification of group B streptococci from selective broth medium by slide co-agglutination test.

Reverse genetics technology so far established for measles virus (MeV) is based on the Edmonston strain, which was isolated several decades ago, has been passaged in nonlymphoid cell lines, and is no...Full Text Available

2000-07-01

295

Rapid Detection of Ofloxacin Resistance in Mycobacterium tuberculosis by Two Low-Cost Colorimetric Methods: Resazurin and Nitrate Reductase Assays

Direct identification of group B streptococci from a selective broth medium was performed with the Phadebact streptococcus test to determine the feasibility of this technique for early detection of...Full Text Available

1978-10-01

296

Pyruvate Dehydrogenase Complex from Germinating Castor Bean Endosperm ¹

We have evaluated the performance of two rapid, low-cost methods for the detection of ofloxacin (OFX) resistance with 95 Mycobacterium tuberculosis isolates from countries with high...Full Text Available

2005-04-01

297

Purification and translation of murine mammary tumor virus mRNA's.

Subcellular organelles from castor bean (Ricinus communis) endosperm were isolated on discontinuous sucrose gradients from germinating seeds which were 1 to 7 days postimbibition. Marker...Full Text Available

1980-02-01

298

Purification and properties of penicillinases from two strains of Bacillus licheniformis: a chemical, physicochemical and physiological comparison

We have studied the functions of the intracellular RNAs of mouse mammary tumor virus (MMTV) by purification and translation in vitro. Two major size classes of MMTV RNA, 35S and 24S RNA, were isolated...Full Text Available

1981-07-01

299

Psychosocial Characteristics of Optimum Performance in Isolated ...

1. The penicillinases formed by penicillinase-constitutive mutant strains from two closely related varieties (749 and 6346) of Bacillus licheniformis have been isolated, characterized...Full Text Available

1965-03-01

300

Properties of single nerve fibres that evoke blood flow changes in cat dental pulp

Studies were assigned values based on whether they were anecdotal or ... or family income), work experience, birth order, military or civilian ...... observed with submarine personnel and polar expeditioners (Sandal et al., 1996). ...

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301

Properties of a cell-wall-defective variant of Brucella abortus of bovine origin.

Single nerve fibres innervating tooth pulp were isolated from filaments dissected from the inferior alveolar nerve in 17 anaesthetized cats. The fibres were studied to determine whether electrical stimulation...Full Text Available

2002-08-01

302

Probabilities of a catastrophic waste hoist accident at the Waste Isolation Pilot Plant

The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing...Full Text Available

1980-08-01

303

Primary and secondary structure of the pore-forming peptide of pathogenic Entamoeba histolytica.

This report shows the probability of a catastrophic accident involving the WIPP waste hoist system. Calculations and mitigation to reduce the probability of an accident and to minimize the impact of such an accident should be included. 10 refs., 8 figs., 4 tabs.

1990-01-01

304

Prevalence and Characterization of Human Shigella Infections in Henan Province, China, in 2006 ?

A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was isolated....Full Text Available

1992-10-01

305

Polysaccharides in germination. Xyloglucans (`amyloids') from the cotyledons of white mustard

In 2006, 3,531 fecal samples were collected from patients with diarrhea in Henan Province, China. A total of 467 (13.2%) Shigella strains were isolated and serotyped. Seventy-one...Full Text Available

2011-01-01

306

Phylogenetic and Evolutionary Relationships among Yellow Fever Virus Isolates in Africa

Two xyloglucan fractions have been isolated from the cotyledons of resting white-mustard seeds, the first by extraction with hot EDTA, and the second by subsequent extraction with alkali or lithium...Full Text Available

1971-08-01

307

Phosphotriesters in rat liver deoxyribonucleic acid after the administration of the carcinogen NN-dimethylnitrosamine in vivo.

Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have...Full Text Available

2001-08-01

308

Pharmacology and toxicology of pahayokolide A, a bioactive metabolite from a freshwater species of Lyngbya isolated from the Florida Everglades

After treatment with NN-di[14C]methylnitrosamine, samples of DNA were isolated from rat livers by a conventional phenol procedure and examined for the presence of phosphotriesters. A method of capable...Full Text Available

1975-03-01

309

The genus of filamentous cyanobacteria, Lyngbya, has been found to be a rich source of bioactive metabolites. However, identification of such compounds from Lyngbya...Full Text Available

2004-12-01

310

Patent arterial duct

Past, present, and future of mutagens in cooked foods.

Patent arterial duct (PAD) is a congenital heart abnormality defined as persistent patency in term infants older than three months. Isolated PAD is found in around 1 in 2000 full term infants. A higher...Full Text Available

311

Passive biaxial mechanical properties of isolated canine myocardium.

Mutation assay with Salmonella typhimurium enabled us to detect various types of mutagens in cooked foods. A series of mutagenic heterocyclic amines has been isolated and identified in broiled fish...Full Text Available

1986-08-01

312

Organization of Bacteriophage Tail-Like Particles in Cells of Chromobacterium violaceum

Excised sheets of canine myocardium were subjected to cyclic loading and unloading in the predominant fibre and cross-fibre directions to determine passive mechanical properties. Myocardium under biaxial...Full Text Available

1983-06-01

313

Only Slight Impact of Predicted Replicative Capacity for Therapy Response Prediction

A strain of Chromobacterium violaceum has been isolated which produces bacteriophage tail-like particles in high numbers. The extracellular morphology and the intracellular arrangement...Full Text Available

1972-05-01

314

Nonhuman Primate Model for Listeria monocytogenes-Induced Stillbirths

BackgroundReplication capacity (RC) of specific HIV isolates is occasionally blamed for unexpected treatment responses. However, the role of viral RC in response to antiretroviral...Full Text Available

315

Nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia.

Listeria monocytogenes, isolated from outbreaks in either human or nonhuman primate populations, was administered orally at doses ranging from 10⁶ to 10¹⁰ CFU....Full Text Available

2003-03-01

316

New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.

Forty-eight mutants unable to reduce nitrate were isolated from "cowpea" Rhizobium sp. strain 32Hl and examined for nitrogenase activity in culture. All but two of the mutants had nitrogenase activity...Full Text Available

1977-02-01

317

New Pathogenicity Marker Found in the Plasticity Region of the Helicobacter pylori Genome

A new strain of mouse hepatitis virus (MHV) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. Negative-contrast electron microscopy showed an abundance...Full Text Available

1979-05-01

318

Neuroprotective effect of paeoniflorin on cerebral ischemic rat by activating adenosine A₁ receptor in a manner different from its classical agonists

Comparison of gastric carcinoma and gastritis isolates showed the presence of genes, probably carcinoma associated (JHP947 and JHP940), that are situated in a Helicobacter...Full Text Available

2003-04-01

319

Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

The effects of paeoniflorin (PF), a compound isolated from Paeony radix, on neurological impairment and histologically measured infarction volume...Full Text Available

2005-10-01

320

Molecular epidemiology of Mycobacterium bovis in Texas and Mexico.

BackgroundThe construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections...Full Text Available

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321

Molecular characterization of hepatitis A virus isolates from environmental and clinical samples in Greece

Seventy-nine Mycobacterium bovis isolates recovered from Mexican and Texas cattle were categorized into 16 and 25 distinct types on the basis of IS6110 and direct-repeat fingerprint patterns, respectively....Full Text Available

1996-09-01

322

Molecular and immunological analysis of a polymorphic periplasmic protein of Borrelia burgdorferi.

BackgroundHepatitis A virus (HAV) strains detected in environmental and clinical samples were analysed to characterize the genotypes of HAV circulating in Greece. Fifty (50) sewage...Full Text Available

323

Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolated from the Eye

Borrelia burgdorferi is the causative agent of Lyme disease, a tick-borne spirochetosis with a worldwide prevalence. To assist the categorization and typing of fresh isolates from global foci, we have...Full Text Available

1991-09-01

324

Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum ?-Lactamases Isolated in Rome, Italy?

Purpose:Methicillin-resistant Staphylococcus aureus (MRSA) strains are commonly classified as hospital-acquired (HA) or community-acquired (CA). Typical HA-MRSA...Full Text Available

2011-02-01

325

Mesophyll Cell Protoplasts of Potato

Escherichia coli strains producing extended-spectrum β-lactamases (ESBLs) are a major problem in many different hospitals worldwide, causing outbreaks as well as sporadic infections....Full Text Available

2008-01-01

326

Malate- and Pyruvate-Dependent Fatty Acid Synthesis in Leucoplasts from Developing Castor Endosperm 1

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among...Full Text Available

1977-08-01

327

Light and Dark Controls of Nitrate Reduction in Wheat (Triticum aestivum L.) Protoplasts ¹

Leucoplasts were isolated from the endosperm of developing castor (Ricinis communis) endosperm using a discontinuous Percoll gradient. The rate of fatty acid synthesis was highest...Full Text Available

1992-04-01

328

Large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes.

Protoplasts were isolated from the leaves of nitrate-cultured wheat (Triticum aestivum L. var. Frederick) seedlings. When incubated in the dark, protoplasts accumulated nitrite under...Full Text Available

1982-02-01

329

K⁺ Channels Are Responsible for an Inwardly Rectifying Current in the Plasma Membrane of Mesophyll Protoplasts of Avena sativa 12

A single large plasmid was isolated from multiplasmid-harboring strains Rhizobium leguminosarum 1001 and R. trifolii 5. These single plasmids, as well as the largest plasmid detectable in R. phaseoli...Full Text Available

1981-03-01

330

Isolation, sequence analysis, and physiological properties of enkephalins in the nervous tissue of the shore crab Carcinus maenas L.

In whole-cell recording, the conductance of the plasma membrane of protoplasts isolated from mesophyll cells of leaves of oat (Avena sativa) was greater for inward than outward current....Full Text Available

1992-03-01

331

Isolation, Purification, and Characterization of an Endogenous Root-promoting Factor Obtained from Basal Sections of Pear Hardwood Cuttings

[Leu]- and [Met]enkephalin from thoracic ganglia of the shore crab Carcinus maenas have been purified to homogeneity by a reversed-phase HPLC procedure. Automated gas-phase sequencing revealed a primary...Full Text Available

1991-10-01

332

Isolation of gram-positive rods that resemble but are clearly distinct from Actinomyces pyogenes from mixed wound infections.

Basal segments taken from Old Home and Bartlett pear hardwood cuttings collected at intervals during the rooting period in September were extracted with ethanol and fractionated by paper chromatography...Full Text Available

1967-04-01

333

Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin

Beginning in 1990, gram-positive rods resembling Actinomyces pyogenes were found with increasing frequency in mixed cultures from various infectious processes, most of them from patients with otitis,...Full Text Available

1993-05-01

334

Isolation of a bacterium resembling Pirellula species from primary tissue culture of the giant tiger prawn (Penaeus monodon).

BackgroundChondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as...Full Text Available

335

Isolation of Assimilatory- and Dissimilatory-Type Sulfite Reductases from Desulfovibrio vulgaris

During attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (Penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin...Full Text Available

1991-11-01

336

Isolation and partial characterization of a specific alpha-fetoprotein receptor on human monocytes.

Bisulfite reductase (desulfoviridin) and an assimilatory sulfite reductase have been purified from extracts of Desulfovibrio vulgaris. The bisulfite reductase has absorption maxima...Full Text Available

1973-08-01

337

Isolation and identification of flavonoid (-)-4'-methyl-epigallocatechin of roots from Maytemus obtusifolia Mart. (Celastraceae)

Since a large body of data has suggested a significant role for alpha-fetoprotein (AFP) in the regulation of the immune response at a number of levels, we examined the possibility of a specific receptor...Full Text Available

1992-10-01

338

Isolation and identification of a cobamide coenzyme from the tapeworm Spirometra mansonoides.

Portuguese ... Orig. Title Isolamento e identificacao do flavonoide (-)-4'-metil-epigalocatequina das raizes de Maytenus obtusifolia Mart. (Celastraceae)

2006-05-19

339

Isolation and characterization of a novel lepidopteran-selective toxin from the venom of South Indian red scorpion, Mesobuthus tamulus

A light-sensitive vitamin B12 derivative has been extracted from the adult cestode, Spirometra mansonoides. This corrinoid was identified as the cobamide coenzyme, adenosylcobalamin, by its chromatographic, chemical, and spectral properties. PMID:1003284

1976-12-01

340

Isolation and characterization of a novel gamma-hexachlorocyclohexane-degrading bacterium.

BackgroundScorpion venom contains insect and mammal selective toxins. We investigated the venom of the South Indian red scorpion, Mesobuthus tamulus for the purpose...Full Text Available

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341

Isolation and Characterization of a Novel Nuclear Protein from Pollen Mother Cells of Lily

The natural biotic capacity of soils to degrade gamma-hexachlorocyclohexane (gamma-HCH, lindane) was estimated using an enrichment technique based on the ability of soil bacteria to develop on synthetic...Full Text Available

1996-10-01

342

Intrinsic Factor-mediated Absorption of Cobalamin by Guinea Pig Ileal Cells

Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G₂...Full Text Available

1990-11-01

343

Intra- and extracellular calcium modulates stereocilia stiffness on chick cochlear hair cells.

To investigate the fate of intrinsic factor and cobalamin during cobalamin absorption, we incubated enterocytes isolated from guinea pig ileum for periods of up to 30 min with ⁵⁷Co-labeled...Full Text Available

1983-03-01

344

Intra- and Interspecies Conjugal Transfer of Tn916-Like Elements from Lactococcus lactis In Vitro and In Vivo?

Segments of the chick basilar papilla were isolated and maintained in culture medium. The sensory hair bundle of individual hair cells was observed with light microscopy and stimulated with a water...Full Text Available

1994-02-01

345

Interrelationships in trace-element metabolism in metal toxicities in a cobalt-resistant strain of Neurospora crassa

Tetracycline-resistant Lactococcus lactis strains originally isolated from Polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using...Full Text Available

2009-10-01

346

Interactions of Antibiotics and Methanolic Crude Extracts of Afzelia Africana (Smith.) Against Drug Resistance Bacterial Isolates

A strain of Neurospora crassa was isolated by training the mould to grow on media containing high concentrations of Co²⁺. This strain, the Co^R strain, exhibited...Full Text Available

1973-04-01

347

Inoculation of Scytalidium thermophilum in Button Mushroom Compost and Its Effect on Yield

Infection due to multidrug resistance pathogens is difficult to manage due to bacterial virulence factors and because of a relatively limited choice of antimicrobial agents. Thus, it is imperative to...Full Text Available

348

Infective arthritis secondary to bladder outflow obstruction.

Scytalidium thermophilum isolates in culture, as well as the endogenous strain(s) in mushroom compost, were inactivated at 70°C. This temperature was used to pasteurize composts...Full Text Available

1994-09-01

349

Incidence and Diversity of Potentially Highly Heat-Resistant Spores Isolated at Dairy Farms

We describe two cases of septic arthritis occurring in association with lower urinary tract infection in elderly men. In both cases the organism isolated from both the joint and the urine was Staphylococcus...Full Text Available

1990-12-01

350

In Vitro Antimicrobial Susceptibilities of Streptococcus pneumoniae Clinical Isolates Obtained in Canada in 2002

The presence of highly heat-resistant spores of Bacillus sporothermodurans in ultrahigh-temperature or sterilized consumer milk has emerged as an important item in the dairy industry....Full Text Available

2005-03-01

351

In Vitro Activity of a New Isothiazoloquinolone, ACH-702, against Mycobacterium tuberculosis and Other Mycobacteria?

Empirical treatment is best guided by current surveillance of local resistance patterns. The goal of this study is to characterize the prevalence of antimicrobial nonsusceptibility within pneumococcal...Full Text Available

2004-09-01

352

Immunological characterization of a basement membrane-specific chondroitin sulfate proteoglycan

In this work, we describe the activity of ACH-702 against clinical isolates of Mycobacterium tuberculosis and six different nontuberculous mycobacteria. The MIC₅₀ and MIC₉₀...Full Text Available

2010-05-01

353

Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.

Reichert's membrane, an extraembryonic membrane present in developing rodents, has been proposed as an in vivo model for the study of basement membranes. We have used this membrane as a source for isolation...Full Text Available

1989-12-01

354

Genomic cloning and characterization of a ricin gene from Ricinus communis.

Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium...Full Text Available

1990-03-01

355

Genomic Content of Bordetella pertussis Clinical Isolates Circulating in Areas of Intensive Children Vaccination

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

356

Genome Sequence of Campylobacter jejuni strain 327, a strain isolated from a turkey slaughterhouse

BackgroundThe objective of the study was to analyse the evolution of Bordetella pertussis population and the influence of herd immunity in different areas of the...Full Text Available

357

Genetic studies of fertility and pathogenicity in Magnaporthe grisea (Pyricularia oryzae)

Campylobacter is one of the leading causes of food-borne gastroenteritis and has a high prevalence in poultry. Campylobacter jejuni subsp. jejuni 327 is a subspecies...Full Text Available

358

Gene-enzyme telationships in somatic cells and their organismal derivatives in higher plants. Progress report

Crosses between field isolates of Magnaporthe grisea (anamorph, Pyricularia oryzae Cav. and Pyricularia grisea) have led to the development of fertile laboratory strains that infect goosegrass and/or weeping lovegrass. These strains may now be utilized in a rigorous genetic analysis of host species specificity and general pathogenicity. Attempts to improve the fertility of rice pathogens for the goal of undertaking a genetic analysis of host cultivar specificity have so far been unsuccessful. Crosses between M. grisea strains that infect rice, goosegrass or weeping lovegrass demonstrate that host species specificity differences between field isolates of this fungus in some cases have a complete genetic basis, and in other cases have a simple genetic basis. Crosses between a field isolate of the pathogen that infects weeping lovegrass and a field isolate that infects goosegrass have resulted in the ...

1984-01-01

359

Free Fatty Acids Regulate Two Galactosyltransferases in Chloroplast Envelope Membranes Isolated from Spinach Leaves

Progress is reported in the following subject areas: (1) chemistry of the arogenate molecule; (2) plant enzymology at the organismal level; (3) isolation of regulatory mutants in tobacco; and (4) stability of the haploid state in Nicotiana sylvestris.

1980-04-21

360

Fluorescence amplified fragment length polymorphism for subtyping of genotypes of Acanthamoeba isolated from patients with keratitis

Effects of MgCl₂ and free fatty acids (FFA) on galactolipid:galactolipid galactosyltransferase (GGGT) and UDP-galactose: 1,2-diacylglycerol galactosyltransferase (UDGT) in chloroplast envelope...Full Text Available

1990-10-01

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361

Flow Cytometry of Human Primary Epidermal and Follicular Keratinocytes

Background & objectives:Acanthamoeba keratitis (AK) is a painful and vision-threatening ocular infection. The differentiation of Acanthamoeba...Full Text Available

2011-01-01

362

First Culture Isolation of Borrelia lonestari, Putative Agent of Southern Tick-Associated Rash Illness

Objective: The aim of this study was to characterize using flow cytometry cultured human primary keratinocytes isolated from the epidermis and hair follicles by different methods. Methods:...Full Text Available

363

Factors influencing the in vitro interaction between immunoglobulins and isolated C1: a critical study

Southern tick-associated rash illness (STARI) is a Lyme disease-like infection described in patients in the southeastern and south-central United States, where classic Lyme disease is relatively rare....Full Text Available

2004-03-01

364

Evidence of Segregated Spawning in a Single Marine Fish Stock: Sympatric Divergence of Ecotypes in Icelandic Cod?

The C1 fixation test is widely used for the study of the interaction between immunoglobulins, their fragments and the complement system. Some factors influencing the apparent extent of the C1 fixation...Full Text Available

1978-05-01

365

Ethnopharmacological evaluation of radal (leaves of Lomatia hirsuta) and isolation of 2-methoxyjuglone

There is increasing recognition of intraspecific diversity and population structure within marine fish species, yet there is little direct evidence of the isolating mechanisms that maintain it or documentation...Full Text Available

366

Epidemiological Risk Factors for Isolation of Ceftriaxone-Resistant versus -Susceptible Citrobacter freundii in Hospitalized Patients

BackgroundLeaves of Lomatia hirsuta are used in traditional medicine in Chile under the common name of "radal". A tea of radal is traditionally used for treatment...Full Text Available

367

Enzymic Capacities of Purified Cauliflower Bud Plastids for Lipid Synthesis and Carbohydrate Metabolism ¹

Antimicrobial resistance is an emerging problem among nosocomial bacteria. Risk factors for the recovery of ceftriaxone-resistant (CRCF) or -susceptible (CSCF) Citrobacter freundii...Full Text Available

2003-09-01

368

Energy Performance Contracting for New Buildings

Isolated cauliflower (Brassica oleracea) bud plastids, purified by isopycnic centrifugation in density gradients of Percoll, were found to be highly intact, to be practically devoid...Full Text Available

1985-10-01

369

Wastenet

situ by isolating portions of the building envelope and performing tests .MAXIMUM ALLOWED LOAD ...and shall identify commissioning team members who will perform testing and prepare documentation.The commissioning agent may be

370

Electron Spin Resonance Studies of Ionic Permeability Properties of Thylakoid Membranes of Beta vulgaris and Avicennia germinans1

Measurement of intrathylakoid aqueous volumes by electron spin resonance spectroscopy was used to study ionic permeability properties of thylakoid membranes isolated from Beta vulgaris...Full Text Available

1985-05-01

371

Effects of ouabain on isolated cerebral and femoral arteries of the cat: a functional and biochemical study.

In recent years, Salmonella enteritidis has become an increasingly important public health problem in Italy. In some parts of the country, the fraction of total human salmonella isolates...Full Text Available

1993-04-01

372

Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells

1. This study analyzes the mechanisms involved in the responses to ouabain in cat cerebral and femoral arteries and characterizes the electrogenic Na+ pump present in these vessels. The latter was accomplished...Full Text Available

1988-01-01

373

Effects of Naja nivea venom on nerve, cardiac and skeletal muscle activity of the frog

BackgroundPerfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells...Full Text Available

2010-01-01

374

Effect of physiological levels of caffeine on Ca²⁺ handling and fatigue development in Xenopus isolated single myofibers

1. The effects of 1 mg/ml whole Naja nivea (Cape cobra) venom, and of three different toxins isolated from it, on spinal reflex excitability and cardiac and skeletal muscle activity...Full Text Available

1973-03-01

375

Distribution, Frequency, and Diversity of Bacillus thuringiensis in an Animal Feed Mill

The purpose of the present study was to determine whether exposure to exogenous physiological concentrations of caffeine influence contractility, Ca²⁺ handling, and fatigue development...Full Text Available

2009-05-01

376

Distribution Frequency of Pathogenic Bacteria Isolated from Cutaneus Leishmaniasis Lesions

Bacillus thuringiensis was isolated from 36 of 50 residue samples obtained from an animal feed mill (a stored-product environment). Of 710 selected colonies having Bacillus...Full Text Available

1992-04-01

377

Dissemination of CTX-M-Type Extended-Spectrum ?-Lactamase Genes to Unusual Hosts

Cutaneous leishmaniasis (CL) is a parasitic disease characterized by single or multiple ulcerations. Secondary bacterial infections are one of the complications that can increase the tissue destruction...Full Text Available

2008-09-01

378

Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae.

A Citrobacter amalonaticus and a Morganella morganii producing the CTX-M-1 extended-spectrum β-lactamase (ESBL) were isolated from an area where this enzyme...Full Text Available

2005-08-01

379

Detection of plasmid-mediated IMP-1 metallo-?-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate

We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen...Full Text Available

1992-11-11

380

Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp

Objective: To investigate the mechanism of carbapenem resistance and the occurrence of plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr...Full Text Available

2009-05-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

DNA replication in Escherichia coli mutants that lack protein HU.

A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility...Full Text Available

1990-11-01

382

Creating genetically modified pigs by using nuclear transfer

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

1989-10-01

383

Coryneform bacteria in infectious diseases: clinical and laboratory aspects.

Nuclear transfer (NT) is a procedure by which genetically identical individuals can be created. The technology of pig somatic NT, including in vitro maturation of oocytes, isolation and treatment of...Full Text Available

384

Computer-based analysis of Haemophilus parasuis protein fingerprints

Coryneform isolates from clinical specimens frequently cannot be identified by either reference laboratories or research laboratories. Many of these organisms are skin flora that belong to a large number...Full Text Available

1990-07-01

385

Complete Sequencing of pNDM-HK Encoding NDM-1 Carbapenemase from a Multidrug-Resistant Escherichia coli Strain Isolated in Hong Kong

AbstractThe present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the...Full Text Available

2004-01-01

386

Comparison of membrane filters for recovery of legionellae from water samples.

BackgroundThe emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to...Full Text Available

387

Comparison of Genotypes of Salmonella enterica Serovar Enteritidis Phage Type 30 and 9c Strains Isolated during Three Outbreaks Associated with Raw Almonds?

The procedure currently used for isolating legionellae from environmental samples recommend filtration through a 0.2-microns-pore-size polycarbonate filter. In this study we evaluated the performance...Full Text Available

1993-01-01

388

Comparison of Disulfide Contents and Solubility at Alkaline pH of Insecticidal and Noninsecticidal Bacillus thuringiensis Protein Crystals

In 2000 to 2001, 2003 to 2004, and 2005 to 2006, three outbreaks of Salmonella enterica serovar Enteritidis were linked with the consumption of raw almonds. The S....Full Text Available

2010-06-01

389

Comparative in vitro activity of Meropenem, Imipenem and Piperacillin/tazobactam against 1071 clinical isolates using 2 different methods: a French multicentre study

We compared two insecticidal and eight noninsecticidal soil isolates of Bacillus thuringiensis with regard to the solubility of their proteinaceous crystals at alkaline pH values. The...Full Text Available

1994-10-01

390

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis.

BackgroundMeropenem is a carbapenem that has an excellent activity against many gram-positive and gram-negative aerobic, facultative, and anaerobic bacteria. The major objective...Full Text Available

391

Comparative Transcriptional and Genomic Analysis of Plasmodium falciparum Field Isolates

The in vitro antibacterial activities of the newly developed 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against 67 clinical isolates of Bacteroides fragilis and their...Full Text Available

1984-01-01

392

Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV.

Mechanisms for differential regulation of gene expression may underlie much of the phenotypic variation and adaptability of malaria parasites. Here we describe transcriptional variation among culture-adapted...Full Text Available

2009-10-01

393

Cholinesterases from Plant Tissues

The cellular receptor for murine coronavirus mouse hepatitis virus (MHV)-A59 is a member of the carcinoembryonic antigen (CEA) family of glycoproteins in the immunoglobulin superfamily. We isolated...Full Text Available

1991-12-01

394

Chlamydial urethral infection in Teheran. A study of male patients attending an STD clinic.

2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride (AMO-1618) inhibits the activity of a cholinesterase isolated from mung bean (Phaseolus aureus) roots...Full Text Available

1973-09-01

395

Chemical structure and inhalation toxicity of lipopolysaccharides from bacteria on cotton.

The prevalence of chlamydial infection of the urethra was studied in 172 consecutive male patients attending a sexually transmitted disease clinic in Teheran. Chlamydia trachomatis was isolated in 8.8%...Full Text Available

1982-12-01

396

Charge compensation for NADPH oxidase activity in microglia in rat brain slices does not involve a proton current

Lipopolysaccharides from different bacteria isolated from cotton were purified and chemically analyzed. Their pulmonary toxicity to animals was tested in inhalation tests. Lipopolysaccharides from Agrobacterium...Full Text Available

1980-09-01

397

Characterization, phylogeny, alternative splicing and expression of Sox30 gene

The membrane properties of isolated cultured microglia have been extensively studied but it is important to understand their properties in situ, where they protect the brain against...Full Text Available

2008-09-01

398

Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family.

BackgroundMembers of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including...Full Text Available

399

Characterization of the 5'-terminal cap structures of early simian virus 40 mRNA.

The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase...Full Text Available

1985-07-01

400

Characterization of group A streptococcal T-12 protein purified by ion-exchange column chromatography.

Early simian virus 40-specific mRNA was isolated from lytically infected and stably transformed cells and analyzed with respect to the 5'-terminal cap content. An analogous diversity of capped structures...Full Text Available

1980-09-01

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401

Characterization of Two Unique Cholesterol-Rich Lipid Particles Isolated from Human Atherosclerotic Lesions

The aim of the present study was to describe the physicochemical characteristics of streptococcal T antigen. T protein isolated from Streptococcus pyogenes type 12 (R53/1077, Colindale) and purified...Full Text Available

1978-09-01

402

Characterization of Terfestatin A, a New Specific Inhibitor for Auxin Signaling1[w

The authors' laboratory, using histochemicalmethods, previously identified two types of cholesterol-containing lipid particles in the extracellular spaces of human atherosclerotic lesions, one particle...Full Text Available

1990-01-01

403

Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia

Terfestatin A (TrfA), terphenyl-β-glucoside, was isolated from Streptomyces sp. F40 in a forward screen for compounds that inhibit the expression of auxin-inducible...Full Text Available

2005-10-01

404

Characteristics of Galacturonic Acid Oligomers as Elicitors of Casbene Synthetase Activity in Castor Bean Seedlings 1

The emergence of Escherichia coli that produce extended spectrum β-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems....Full Text Available

2009-01-01

405

Changes in Rabbit and Cow Lens Shape and Volume upon Imposition of Anisotonic Conditions

Partial digestion of polygalacturonic acid with polygalacturonase isolated from Rhizopus stolonifer produces a mixture of α-1,4-d-galacturonide oligomers which act...Full Text Available

1984-04-01

406

Capacity of staphylococci to grow in the presence of ovotransferrin or CrCl3 as a character of potential pathogenicity.

In vivo, mammalian lenses have the capacity to effect fully reversible changes in shape, and possibly volume, during the accommodation process. Isolated lenses also change shape...Full Text Available

2009-10-01

407

CTX-M-Type Extended-Spectrum ?-Lactamase That Hydrolyzes Ceftazidime through a Single Amino Acid Substitution in the Omega Loop

A total of 150 strains of staphylococci, clinically isolated, were tested for sensitivity to ovotransferrin (conalbumin). Among these, all the 50 coagulase-positive, mannitol-positive, and deoxyribonuclease-positive...Full Text Available

1980-05-01

408

CTX-M ?-Lactamases in Escherichia coli from Community-acquired Urinary Tract Infections, Cambodia

Escherichia coli ILT-1, Klebsiella pneumoniae ILT-2, and K. pneumoniae ILT-3 were isolated in May 1999 in Paris, France, from a rectal swab of a hospitalized...Full Text Available

2001-12-01

409

CHRONIC ETHANOL FEEDING AFFECTS PROTEASOME INTERACTING PROTEINS

Despite the recent global spread of CTX-M β-lactamases in Escherichia coli isolates from community-acquired urinary tract infections (CA-UTIs), their dissemination has been...Full Text Available

2009-05-01

410

Buoyant Densities and Dry-Matter Contents of Microorganisms: Conversion of a Measured Biovolume into Biomass

Studies on alcoholic liver injury mechanisms show a significant inhibition of the proteasome activity. To investigate this phenomenon, we isolated proteasome complexes from the liver of rats...Full Text Available

2009-07-01

411

Blarina toxin, a mammalian lethal venom from the short-tailed shrew Blarina brevicauda: Isolation and characterization

Several isolates of bacteria and fungi from soil, together with cells released directly from soil, were studied with respect to buoyant density and dry weight. The specific volume (cubic centimeters...Full Text Available

1983-04-01

412

Biological Response Modifier Activity of an Exopolysaccharide from Paenibacillus jamilae CP-7

Venomous mammals are rare, and their venoms have not been characterized. We have purified and characterized the blarina toxin (BLTX), a lethal mammalian venom with a tissue kallikrein-like activity...Full Text Available

2004-05-18

413

Barks of terminalia species - a new source of oxalic acid

An extracellular polysaccharide was purified from culture supernatants of Paenibacillus jamilae CP-7, a gram-positive bacillus that was isolated from compost prepared with olive mill...Full Text Available

2001-07-01

414

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions?

The digestion of water-extracted bark of Terminalia alata nepalensis, T. arjuna, and T. bellerica with 20% H/sub 2/SO/sub 4/ at 100 degrees resulted in the isolation of oxalic acid in 12-21% yield. The technique is not complicated and requires simple equipment.

1980-01-01

415

Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)

This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found...Full Text Available

2009-03-01

416

Antimicrobial susceptibilities of bacteria associated with periodontal disease.

Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess...Full Text Available

417

Antibacterial Colorants: Characterization of Prodiginines and Their Applications on Textile Materials

A total of 193 bacterial strains were tested for their susceptibilities to 14 antimicrobial agents. Penicillin G was active at 2 U/ml against 98% of the oral isolates. Other antibiotics with good activity...Full Text Available

1983-03-01

418

Anti-beta-endorphin immunoglobulin G in humans.

A strain of Vibrio sp. isolated from marine sediments produced large quantities of bright red pigments that could be used to dye many fibers including wool, nylon, acrylics,...Full Text Available

2008-05-01

419

Anthracenedione Derivatives as Anticancer Agents Isolated from Secondary Metabolites of the Mangrove Endophytic Fungi

Human IgG specific for beta-endorphin was identified by enzyme-linked immunoabsorbent assay and isolated by affinity chromatography. From a sample of 27 subjects, three individuals with major depression...Full Text Available

1986-11-01

420

Analysis of non-TIR NBS-LRR resistance gene analogs in Musa acuminata Colla: Isolation, RFLP marker development, and physical mapping

In this article, we report anticancer activity of 14 anthracenedione derivatives separated from the secondary metabolites of the mangrove endophytic fungi Halorosellinia sp. (No. 1403)...Full Text Available

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421

An Analysis of the Subunit Structure of the Crystalloid Protein Complex from Castor Bean Endosperm 1

BackgroundMany commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. Fertile wild relatives,...Full Text Available

422

Amorphigenin inhibits Osteoclast differentiation by suppressing c-Fos and nuclear factor of activated T cells

Chromatographic and electrophoretic studies have shown that the subunits of the crystalloid protein, isolated from mature castor bean (Ricinus communis L. cv Hale) seed endosperm protein...Full Text Available

1983-06-01

423

Activity of Four Cephalosporin Antibiotics In Vitro Against Bovine Udder Pathogens and Pathogenic Bacteria Isolated from Newborn Calves

Among the several rotenoids, amorphigenin is isolated from the leaves of Amopha Fruticosa and it is known that has anti-proliferative effects and anti-cnacer effects in many cell types....Full Text Available

2010-12-01

424

A new gene (alkB) of Escherichia coli that controls sensitivity to methyl methane sulfonate.

The in vitro activity of chephaloridine, cephalexin, cefatrizine (BL-S640), and cephapirin (BL-P-1322) was evaluated by the serial dilution method against pathogenic gram-positive and gram-negative...Full Text Available

1976-03-01

425

A molecular epidemiological study of rabies epizootics in kudu (Tragelaphus strepsiceros) in Namibia

Seven mutants of Escherichia coli were isolated that are sensitive to methyl methane sulfonate but not to UV light. They exhibited decreased host cell reactivation capacity for methyl methane sulfonate-treated...Full Text Available

1983-03-01

426

A cytochrome P450 terpenoid hydroxylase linked to the suppression of insect juvenile hormone synthesis

BackgroundA panel of 37 rabies virus isolates were collected and studied, originating mainly from the northern and central regions of Namibia, between 1980 and 2003.ResultsThese...Full Text Available

427

A Suppression Subtractive Hybridization Approach Reveals Niche-Specific Genes That May Be Involved in Predator Avoidance in Marine Synechococcus Isolates

A cDNA encoding a cytochrome P450 enzyme was isolated from a cDNA library of the corpora allata (CA) from reproductively active Diploptera punctata cockroaches. This P450 from the endocrine...Full Text Available

1998-10-27

428

A Nitrate Reductase-less Variant Isolated from Suspension Cultures of Datura innoxia (Mill.) ¹

Picocyanobacteria of the genus Synechococcus are important contributors to marine primary production and are ubiquitous in the world's oceans. This genus is genetically diverse, and...Full Text Available

2006-04-01

429

A Bacillus thuringiensis isolation method utilizing a novel stain, low selection and high throughput produced atypical results

A comparative study has been carried out of the growth of two lines of Datura innoxia (Mill.) cells, designated DI-6 and NR1, their resistance to chlorate, and their ability to assimilate...Full Text Available

1980-10-01

430

[The metabolites of cyclic peptides from three endophytic mangrove fungi].

BackgroundBacillus thuringiensis is a bacterium known for producing protein crystals with insecticidal properties. These toxins are widely sought after for controlling...Full Text Available

431

Yucca Mountain, Nevada - A Proposed Geologic Repository for High-Level Radioactive Waste (Volume 1) Introduction

Nine secondary metaboites of cyclic peptide were isolated from three mangrove endophytic fungi Paecilomyces sp. (treel-7), 4557,ZZF65. They were viscumamide(1),cyclo(Pro-Iso)(2),cyclo(Phe-Gly)(3),cyclo(Phe-Ana)(4),cyclo(Gly-Pro) (5),cyclo(Gly-Leu)(6), cyclo(Trp-Ana)(7),neoechinulin A(8),cyclo(Pro-Thr)(9). The compounds 1,7,8,9 were firstly isolated from marine fungus. PMID:18422185

2007-12-01

432

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

Yucca Mountain in Nevada represents the proposed solution to what has been a lengthy national effort to dispose of high-level radioactive waste, waste which must be isolated from the biosphere for tens of thousands of years. This chapter reviews the background of that national effort and includes some discussion of international work in order to provide a more complete framework for the problem of waste disposal. Other chapters provide the regional geologic setting, the geology of the Yucca Mountain site, the tectonics, and climate (past, present, and future). These last two chapters are integral to prediction of long-term waste isolation.

2006-09-25

433

Stability and chaotification of vibration isolation floating raft systems with time-delayed feedback control

We have isolated a genomic clone containing 'Lupinus luteus' 5S ribosomal RNA genes by screening with 5S rDNA probe clones that were hybridized previously with the initiator methionine tRNA preparation (contaminated) with traces of rRNA or its degradation products). The clone isolated contains ten repeat units of 342 bp with 119 bp fragment showing 100% homology to the 5S rRNA from yellow lupine. Sequence analysis indicates only point heterogeneities among the flanking regions of the genes. (author). 6 refs, 3 figs.

1993-01-01

434

Simple method for the high-temperature isolation of /sup 111/In from silver

This paper presents a systematic study on the stability of a two-dimensional vibration isolation floating raft system with a time-delayed feedback control. Based on the generalized Sturm criterion, the critical control gain for the delay-independent stability region and critical time delays for the stability switches are derived. The critical conditions can provide a theoretical guidance of chaotification design for line spectra reduction. Numerical simulations verify the correctness of the approach. Bifurcation analyses reveal that chaotification is more likely to occur in unstable region defined by these critical conditions, and the stiffness of the floating raft and mass ratio are the sensitive parameters to reduce critical control gain.

2011-09-01

435

Patient isolation in chronic benign pain.

A method is proposed for the quantitative high-temperature isolation of /sup 111/In from silver irradiated by ..cap alpha..-particles into a gas phase containing a mixture of HCl and H/sub 2/O with a total pressure of about 3 Pa for these gases. It is shown that the indium evolved into the gas phase probably occurs in the form of at least three compounds, viz.., the trichloride, monochloride, and oxychloride, which have adsorption temperatures on quartz of around 385, 475, and 655 K, respectively. All these compounds are readily washed from the surface of quartz apparatus by a weak (0.05 M) solution of hydrochloric acid.

1987-07-01

436

On restricted Analytic Gradients on Analytic Isolated Surface Singularities

Over the years, nurses, doctors and scientists have sought ways of assessing the impact of pain on patients. While many of the instruments devised have been extremely useful in identifying the nature and intensity of pain, defining the effect it has on people's lives and perceptions has proved much more problematic. Based on the work she carried out with patients suffering from chronic benign pain, Karen Rose explores one aspect of these effects--the feeling of isolation. By using a four-step anthropological approach to identify the impact of pain, she argues, nurses can arrive at a realistic and accurate assessment of how patients are coping. PMID:7947167

437

CERN Document Server

Let (X,O) be a real analytic isolated surface singularity at the origin o of a real analytic manifold M equipped with a real analytic metric g. Given a real analytic function f:(M,O) --> (R,0) singular at O, we prove that the gradient trajectories for the metric g|(X,O) of the restriction f|X escaping from or ending up at the origin O do not oscillate. Such a trajectory is thus a sub-pfaffian set. Moreover, in each connected component of X\\O where the restricted gradient does not vanish, there is always a trajectory accumulating at O and admitting a formal asymptotic expansion at O

2011-01-01

438

Metabolism of benzo(a)pyrene by isolated perfused testis and testicular homogenate

Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.

In an effort to improve the extrapolation of laboratory data to man and estimate risk of human reproductive toxicity associated with environmental exposure, the pharmacokinetic parameters of the testicular compartment are being studied. Of particular interest is the variety of enzyme systems capable of activating and detoxicating environmental chemicals and drugs. This report compares the metabolism of benzo(a)pyrene by the isolated perfused testis and testicular homogenates in vitro. The cell free in vitro system metabolized benzo(a)pyrene at a much greater rate than the perfused testis and produced a different spectrum of metabolites. Reliable laboratory prediction of biotransformation by the whole organ or intact animal is an essential aspect of reproductive toxicology.

1980-12-01

439

Isolated laevocardia and corrected transposition of the great vessels with total situs inversus

A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes. PMID:4091794

1985-12-01

440

DECOVALEX - Mathematical models of coupled T-H-M processes for nuclear waste repositories. Executive summary for Phases I,II and III

Isolated laevocardia combined with corrected transposition of the great arteries and complete situs inversus is rare. Due to discordant interrelationships between atria and ventricles and between ventricles and great arteries, both anomalies result in a normal circulation. In the presented case no associated cardiac malformations were present and the 55-year-old woman remained symptom-free for a long time until she developed an acquired valve disease. A systematic approach to chamber localisation in complex morphologic lesions of the heart is reviewed, and the value of CT, which can be applied successfully in older children and adults is demonstrated. (orig.).

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441

Chemical constituents of Galianthe brasiliensis (Spreng.) E.L.Cabral and Bacigalupo (Rubiaceae)

This executive summary presents the motivation, structure, objectives, methodologies and results of the first stage of the international DECOVALEX project - DECOVALEX I (1992-1995). The acronym stands for Development of Coupled Models and their Validation against Experiment in Nuclear Waste Isolation, and the project is an international effort to develop mathematical models, numerical methods and computer codes for coupled thermo-hydro-mechanical processes in fractured rocks and buffer materials for geological isolation of spent nuclear fuel and other radioactive wastes, and validate them against laboratory and field experiments. 24 refs.

1996-06-01

442

Source tracking of Escherichia coli by 16S-23S intergenic spacer region denaturing gradient gel electrophoresis (DGGE) of the rrnB ribosomal operon.

This paper describes the chemical constituents isolated from aerial parts of the plant Galianthe brasiliensis. From a methanol extract, the iridoid glycosides asperuloside, deacetylasperuloside, mixture of Z- and E-6-O-p-coumaroylscandoside methyl ester, the triterpene ursolic acid and the steroids stigmasterol, campesterol, b-sitosterol and 3-O-b-glucopyranosyl sitosterol were isolated. The structures of the natural products were identified on the basis of spectral data, including 2D NMR experiments. The antiproliferative properties of the crude methanolic extract were investigated against a series of nine human cancer cell lines. (author)

443

Isolation of imidacloprid degrading bacteria from industrial sites

This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjected to DGGE to determine sequence variations within the 16S-23S intergenic spacer region (ISR) of the rrnB ribosomal operon. The ISR was analyzed to determine if E. coli isolates from various animal sources could be differentiated from each other. DNA isolated from the E. coli animal sources was PCR amplified to isolate the rrnB operon. To prevent amplification of all 7 E. coli ribosomal operons by PCR amplification using universal primers, sequence-specific primers were utilized for the rrnB operon. Another primer set was then used to prepare samples of the 16S-23S ISR for DGGE. Comparison ...

2007-10-01

444

Responses of hybrid poplar clones and red maple seedlings to ambient O_3 under differing light within a mixed hardwood forest

Immidacloprid is a cyclodiene organochlorine used as an insecticide all over the world and possessing a serous environmental threat. It is mostly used for cotton insects (bollworm, aphid and white fly). For isolation of imidacloprid degrading bacteria, two soil samples were collected from industrial contaminated sites of Kala Shah Kahu district sheikupura, having ten year history of use. Soil samples were analyzed by measuring pH and electric conductivity. The isolation of imidacroprid degrading bacteria was performed by enrichment technique. Eight bacterial strains, S/sub 1-a/ S/2-2-b/ S/2-c/ S/2-d/ S/2-e/ S/sub 2-f/ and S/sub 2-g/ and S/sub e-a/ were isolated on the basis of their colony morphologies. The purified colonies were characterized morphologically, physiologically and biochemically. Gram staining was done and Gram negative strain were confirmed on MacConkey agar and Eosin Methylene Blue. Bacterial strains were ...

445

[Peritonitis in the course of peritoneal dialisis caused by Haemophilus influenzae with BLNAR phenotype].

The responses of ramets of hybrid poplar (Populus spp.) (HP) clones NE388 and NE359, and seedlings of red maple (Acer rubrum, L.) to ambient ozone (O_3) were studied during May-September of 2000 and 2001 under natural forest conditions and differing natural sunlight exposures (sun, partial shade and full shade). Ambient O_3 concentrations at the study site reached hourly peaks of 109 and 98 ppb in 2000 and 2001, respectively. Monthly 12-h average O_3 concentrations ranged from 32.3 to 52.9 ppb. Weekly 12-h average photosynthetically active radiation (PAR) within the sun, partial shade and full shade plots ranged from 200 to 750, 50 to 180, and 25 to 75 #mu#mol m"-"2 s"-"1, respectively. Ambient O_3 exposure induced visible foliar symptoms on HP NE388 and NE359 in both growing seasons, with more severe injury observed on NE388 than on NE359. Slight foliar symptoms were observed on red maple seedlings during the 2001growing season. Percentage of total leaf area ...

2004-07-01

446

[Development of efficient DNA isolation procedures for Cryptosporidium and Trichinella PCR detection in fecal samples].

Most common bacterial species causing peritonitis in the course of peritoneal dialysis (PDP) are coagulase-negative staphylococci, Staphylococcus aureus and streptococci. Haemophilus influenzae is rarely associated with PDP. Hereby we present the first known case of APD-associated peritonitis caused by non-type able H. influenzae (NTHi) presenting the beta-lactamase negative, ampicillin-resistant (BLNAR) phenotype. An 18 year old boy who had been treated with the APD for 12 months due to SLE was admitted in good general condition with diagnosis of PDP. Standard diagnostic and therapeutical procedures were initiated. Dialysis fluid was turbid with cytosis of 435 WBC/ml. From dialysis fluid pure culture of Gram-negative coccobacillus was isolated. The isolate was identified as a BLNAR phenotype. The same bacterium was isolated from nasal swab. Blood cultures were negative. After evaluation of antimicrobial susceptibility the ...

2009-04-01

447

Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes

PCR detection of genetic material of the parasites present in faeces may be an alternative for microscopic and serological tests routinely used for diagnosing parasitic enteral infections. However, small amount of target DNA combined with low efficiency of total DNA extraction, and presence of PCR inhibitors in the samples to be amplified, may cause false negative detection results. The aim of this work was to evaluate the impact of DNA isolation procedure used on the amplification of DNA fragments from the genomes of protozoan Cryptosporidium parvum and the nematode Trichinella spiralis. Two methods based on different principles of biological material lysis were evaluated; NucliSENS miniMAG employing simultaneously applied chemical lysis and mechanical disruption or mechanical disruption followed by enzymatic lysis in case of QIAamp DNA Stool Mini Kit. Both of the analyzed systems for nucleic acids purification allowed isolation of DNA from ...

2009-01-01

448

Improved oil recovery using bacteria isolated from North Sea petroleum reservoirs

To study the regulation of adipose tissue lipoprotein lipase (LPL) in human adipocytes, omental adipose tissue was obtained from healthy subjects and digested in collagenase. The isolated adipocytes thus obtained were suspended in Medium 199 and cultured at 37 degrees C. Cell viability was demonstrated in adipocytes cultured for up to 72 h by constancy of cell number, cell size, trypan-blue exclusion, and specific /sup 125/I-insulin binding. In addition, chloroquine induced an increase in cell-associated /sup 125/I-insulin at 24, 48, and 72 h after preparation. Thus, isolated adipocytes retained their ability to bind, internalize, and degrade insulin. LPL was measured as activity secreted into the culture medium (CM), released from cells by heparin (HR), and extracted from cell digests. A broad range of heparin concentrations produced a prompt release of LPL from a rapidly replenishable pool of cellular activity. When cells were cultured in ...

1985-01-01

449

Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals.

During secondary oil recovery, water is injected into the formation to sweep out the residual oil. The injected water, however, follows the path of least resistance through the high-permeability zones, leaving oil in the low-permeability zones. Selective plugging of these their zones would divert the waterflood to the residual oil and thus increase the life of the well. Bacteria have been suggested as an alternative plugging agent to the current method of polymer injection. Starved bacteria can penetrate deeply into rock formations where they attach to the rock surfaces, and given the right nutrients can grow and produce exo-polymer, reducing the permeability of these zones. The application of microbial enhanced oil recovery has only been applied to shallow, cool, onshore fields to date. This study has focused on the ability of bacteria to enhance oil recovery offshore in the North Sea, where the environment can be considered extreme. A screen of produced water from oil reservoirs (and ...

1995-12-31

450

A study of bacterial isolates from corneal specimens and their antibiotic resistance profile

From January 2008 to December 2009, 433 Streptococcus pneumoniae strains were examined to determine the serotype distribution and susceptibility to selected antibiotics. About 50% of them were invasive isolates. The strains were isolated from patients of all age groups and 33.55% were isolated from children below 5 years. The majority was isolated from blood (48.53%) and other sterile specimens (6.30%). Community acquired pneumonia (41.70%) is the most common diagnosis followed by sepsis (9.54%). Serotyping was done using Pneumotest Plus-Kit and antibiotic susceptibility pattern was determined by modified Kirby-Bauer disk diffusion method and measurement of minimum inhibitory concentration (MIC) using E-test strip. Ten most common serotypes were 19F (15.02%), 6B (10.62%), 19A (6.93%), 14 (6.70%), 1 (5.08%), 6A (5.08%), 23F (4.85%), 18C (3.93%), 3 (2.08%) and 5 (1.85%). Penicillin MIC ranged between ? ...

2011-06-30

451

Species differences in anxiety-related responses in male prairie and meadow voles: the effects of social isolation.

We aim to examine the spectrum of bacteria causing corneal infections and their antibiotic susceptibility patterns. This will serve as a guideline for empiric therapy of corneal infections. We conducted the study over a period of 18 months from March 2001 through December 2002 in King Abdul-Aziz University Hospital, Riyadh, Kingdom of Saudi Arabia. Corneal specimens taken from 200 patients were inoculated directly onto different types of media. The isolates were identified and then tested against the appropriate topical or systemic antibiotics. Sixty-seven (33.5%) of the total specimens were culture positive and 133 (66.5%) were culture negative. Fourteen (7%) of these showed organisms in the Gram stained smears and correlated well with the culture reports. Of the 67 positive cultures, 53 (79.1%) were Gram-positive bacteria mostly coagulase-negative Staphylococci 29 (43.3%) followed by Streptococcus pneumoniae (S. pneumoniae) 13 (19.4%). Among Gram-negative ...

452

Serotypes and susceptibilities of paediatric clinical isolates of Streptococcus pneumoniae in Crete, Greece, before and after the heptavalent pneumococcal conjugate vaccine.

Prairie (Microtus ochrogaster) and meadow voles (M. pennsylvanicus) are closely related species that differ in life strategy and social behaviors, and thus provide an excellent comparative model for the study of neuronal and hormonal mechanisms underlying behavior. In the present study using the elevated plus maze (EPM) test, we found that male prairie voles entered the open arms of the EPM more and remained there longer, and showed a higher level of overall locomotor activity than did male meadow voles. In addition, two weeks of social isolation induced an increase in open arm entries in prairie, but not meadow, voles. Prairie voles also had a higher level of circulating corticosterone compared to meadow voles, and the EPM test increased circulating corticosterone in prairie voles. Finally, social isolation coupled with the EPM test influenced Fos-immunoreactive expression in several brain areas, including the medial preoptic area, ...

2005-08-22

453

Radon-mitigation in buildings

All Streptococcus pneumoniae strains isolated from paediatric clinical samples at Heraklion University General Hospital in the 10-year period 2000-2009 were tested for serotype and susceptibility to antimicrobials. Among a total of 258 strains, 159 were isolated in the 5-year period 2000-2004, before the introduction of the heptavalent pneumococcal conjugate vaccine (PCV7), and 99 in the post-PCV7 5-year period 2005-2009. The prevalence of PCV7-included serotypes decreased in the post-PCV7 period (p?=?0.0002), but an increase was observed for serotypes 7F (p?=?0.002) and 19A (p?=?0.004). Pan-susceptibility rates and susceptibility to cotrimoxazole increased in the post-PCV7 period (p?=?0.01 and p?=?0.008, respectively), but serotype 19A emerged as a contributor to multi-resistance (p?=?0.007). PCV7 was followed by decreased S. pneumoniae resistance and prevalence of vaccine-related serotypes but increased prevalence of serotypes 7F and 19A. ...

2010-07-10

454

Nuclear magnetic resonance spectroscopy in the structure elucidation and biosynthesis of natural products

From the indoor radon problem arises a great challenge for the radiation protection of the population. The soil can be considered as the only source for high radon concentrations in buildings. The contribution of common building materials to high indoor concentrations with a range of 30 Bq/m"3 is negligible low. Therefore building materials for radon mitigation should be judged by their tightness against diffusive radon and not by their radon exhalation rate. Also isolation materials for mitigation should be radon tight. The radon diffusion coefficient describes the physical processes in relatively homogeneous samples. The diffusion coefficient D and the diffusion length R are very exactly determined by a self engineered measuring method. The range of D reaches from D = (0.0005 to 2.35) . 10"-"6 m"2/s for building materials and from D = (0.07 to < 10"-"6) . 10"-"6 m"2/s for isolation materials. Applicable mitigation measures like increased ...

455

Local Group Dwarf Galaxies: Nature And Nurture

Examination of a chloroform extract of Dracaena loureiri Gagnep (Agavaceae), a Thia medicinal plant possessing antibacterial activity, has led to the isolation of fifteen flavenoids. The biogenic relationships among these flavenoids isolated were briefly discussed. Definition of the skeleton and the unambiguous assignment of all of the protons of the isolates was achieved through extensive 2D-homonuclear chemical shift correlation, nuclear Overhauser effect (NOE) difference spectroscopy and 2D-NOE experiments. The {sup 1}H and {sup 13}C NMR spectra of staurosporine, a potent biologically active agent from Streptomyces staurosporeus, were unambiguously assigned by using 2D homonuclear chemical shift correlation, NOE, {sup 1}H-detected heteronuclear multiple-quantum coherence via direct coupling and via multiple-bond coupling for resonance assignments of protonated and nonprotonated carbons, respectively. S. Staurosporeus was ...

1988-01-01

456

CERN Document Server

We investigate the formation and evolution of dwarf galaxies in a high resolution, hydrodynamical cosmological simulation of a Milky Way sized halo and its environment. Our simulation includes gas cooling, star formation, supernova feedback, metal enrichment and UV heating. In total, 90 satellites and more than 400 isolated dwarf galaxies are formed in the simulation, allowing a systematic study of the internal and environmental processes that determine their evolution. We find that 95% of satellite galaxies are gas-free at z=0, and identify three mechanisms for gas loss: supernova feedback, tidal stripping, and photo-evaporation due to re-ionization. Gas-rich satellite galaxies are only found with total masses above ~ 5x10^9 solar masses. In contrast, for isolated dwarf galaxies, a total mass of ~ 10^9 solar masses constitutes a sharp transition; less massive galaxies are predominantly gas-free at z=0, more massive, ...

2011-01-01

457

Isolation of fetal DNA from nucleated erythrocytes in maternal blood

Evaluation of fetal brain development by magnetic resonance imaging. Subependymal germinal matrix layer and cerebral ventricle

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. The authors used monoclonal antibody against the transferrin receptor (TIR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, they were successful in detecting the Y chromosomal sequence in 75% of ...

1990-05-01

458

Erratum to: Inhibition of triclabendazole metabolism in vitro by ketoconazole increases disruption to the tegument of a triclabendazole-resistant isolate of Fasciola hepatica

Three dimensional data of brain from the formalin-fixed fetuses were collected without isolation, by the 4.7 tesla super high magnetic field MRI and the developmental process of the cerebral parenchyma was studied by 3D images. Subjects were 13 fetal brain and MRI was performed using 3D-steady-state free precession sequence. The isolated brain is very soft and fragile and is deformed by its weight at the imaging. However 3D-MRI can be obtained without isolation, and the deformation is remarkably small. The subependymal germinal matrix layer did not be observed in 7 weeks-old fetus, appeared at 9 weeks-old and increased gradually. Then it rapidly reduced from 28 weeks-old. The volume calculated, from 3D-MRI, increased rapidly from 9 weeks-old to 23 weeks-old, and reached the maximum (2.346 mm"3) at 23 weeks-old. The relation between fetal ages and volume of cerebral ventricle also showed similar pattern. This method will be ...

1999-10-01

459

British Library Electronic Table of Contents (United Kingdom)

A study has been carried out to investigate whether the action of triclabendazole (TCBZ) against Fasciola hepatica is altered by inhibition of drug metabolism. The cytochrome P450 (CYP 450) enzyme pathway was inhibited using ketoconazole (KTZ) to see whether a TCBZ-resistant isolate could be made more sensitive to TCBZ action. The Oberon TCBZ-resistant and Cullompton TCBZ-susceptible isolates were used for these experiments. The CYP 450 system was inhibited by a 2-h pre-incubation in ketoconazole (40??M), then incubated for a further 22?h in NCTC medium containing either KTZ, KTZ?+?nicotinamide adenine dinucleotide phosphate (NADPH) (1?nM), KTZ?+?NADPH?+?TCBZ (15??g/ml), or KTZ?+?NADPH?+?triclabendazole sulphoxide (TCBZ.SO; 15??g/ml). Changes to fluke ultrastructure following drug treatmen...

2011-01-01

460

Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad

British Library Electronic Table of Contents (United Kingdom)

Abstract The objective of this study was to determine the functional properties of cartilaginous tissues generated by porcine MSCs isolated from different tissue sources, and to compare these properties to those derived from chondrocytes (CCs). MSCs were isolated from bone marrow (BM) and infrapatellar fat pad (FP), while CCs were harvested from the articular surface of the femoro-patellar joint. Culture-expanded CCs and MSCs were encapsulated in agarose hydrogels and cultured in the presence of TGF3. Samples were analysed biomechanically, biochemically and histologically at days 0, 21 and 42. After 42 days in free swelling culture, mean GAG content was 1.50% w/w in CC-seeded constructs, compared to 0.95% w/w in FP- and 0.43% w/w in BM-seeded constructs. Total collagen accumulation was hig...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Types of nonanticlinal oil and gas traps and their dispersal within the island section of North Sakhalin

The isolation of "1"3"9Ce after production by a proton-induced nuclear reaction on praseodymium

Questions are examined of prospecting for nonanticlinical traps within North Sakhalin, their spatial distribution and evaluation of the outlook for oil and gas content. Traps of tapering and facial substitution on wings and periclines of local elevations, accumulative bar and erosion-accumulative channel are isolated. THe hypothesis is made that it is possible to form stratigraphic traps above the discrepancy and below it. It is noted that formation of zones of lithological tapering and facial substitution are associated with areas of the exchange of the sedimentation situation. The primary role of the shore and coastal zones in the formation of the examined types of nonanticlinal traps is established. The Okhino-Ekhabinskiy region in North Sakhalin is isolated as the primary object for prospecting for nonanticlinal traps.

1981-01-01

462

The endophytic fungi from wheat (Triticum aestivum L.)

A method, based on anion exchange chromatography, is presented for the isolation of "1"3"9Ce after production by a proton-induced nuclear reaction on a thick praseodymium target. After bombardment the target is dissolved in nitric acid and "1"3"9Ce oxidised to the Ce(IV) oxidation state with bromic acid. "1"3"9Ce is then separated form the praseodymium by anion exchange chromatography on a Ag MP-1 resin column in a nitric acid - bromic acid mixture. "1"3"9Ce is sorbed onto the resin column and praseodymium eluted with the acid mixture. The bromic acid is washed out of the column with nitric acid and "1"3"9Ce finally eluted with dilute nitric acid containing sulphur dioxide.

2001-02-17

463

British Library Electronic Table of Contents (United Kingdom)

In order to study the species composition of endophytes from wheat healthy plants in Buenos Aires Province (Argentina) and to determine their infection frequencies from leaves, stems, glumes and grains, wheat plants were collected from five cultivars at five growth stages from crop emergence to harvest. A total of 1,750 plant segments (leaves, stems, glumes and grains) were processed from the five wheat cultivars at five growth stages, and 722 isolates of endophytic fungi recovered were identified as 30 fungal genera. Alternaria alternata, Cladosporium herbarum, Epicoccum nigrum, Cryptococcus sp., Rhodotorula rubra, Penicillium sp. and Fusarium graminearum were the fungi that showed the highest colonization frequency (CF%) in all the tissues and organs analysed. The number of taxa isolated...

2007-01-01

464

Studies on the chemical composition and possible mechanisms underlying the antispasmodic and bronchodilatory activities of the essential oil of Artemisia maritima L.

British Library Electronic Table of Contents (United Kingdom)

This study describes the chemical composition of the essential oil of Artemisia maritima (Am.Oil) and the pharmacological basis for its medicinal use in gut and airways disorders. Twenty five compounds, composing 93.7% of the oil, were identified; among these, chrysanthenyl propionate and elixene were identified for the first time from any Artemisia species. The Am.Oil (0.3?1.0 mg/mL) suppressed spontaneous and high K+ (80 mM)-induced contractions in isolated rabbit jejunum, suggestive of an antispasmodic effect mediated possibly through calcium channel blockade. The calcium channel blockade activity was confirmed when pre-treatment of the tissue with Am.Oil (0.01?0.03 mg/mL) shifted the Ca++ concentration-response curves to the right, similar to verapamil and papaverine. In isolated trach...

2011-01-01

465

Studies on the acidity of mordenite and ZSM-5. 2. Loss of Broensted acidity by dehydroxylation and dealumination

Removal of natural humic acids by decolorizing actinomycetes isolated from different soils (Algeria) for application in water purification

Quantitative determination of Broensted acid site concentrations in calcined H-mordenite and H-ZSM-5 by means of conductometric titration and infrared spectroscopy reveals that the concentration of Broensted acid sites present is lower than would be expected on the basis of the aluminum content. This discrepancy is attributed to dealumination and dehydroxylation processes occurring during calcination. A model is proposed according to which the degree of these processes strongly depends on the concentration of vicinal aluminum T sites in the zeolite: Broensted acid sites associated with these T sites are lost, and the remaining Broensted acidity is related to the concentration of the (remaining) isolated aluminum T sites. This model quantitatively correlates the experimentally determined concentration of Broensted acid sites with the concentration of isolated aluminum T sites. 33 refs., 4 figs., 2 tabs.

1993-01-14

466

British Library Electronic Table of Contents (United Kingdom)

Humic acids are considered problematically in drinking water because it can react readily with chlorine to form carcinogen compounds and its biological removal is much recommended. The scanning electron microscopy morphologies and optical parameters observed for natural humic acids (NHAs) extracted from different soils at Mitidja plain (Algeria) made them different from the commercial ones. Three of the most active strains of 19 actinomycetes were isolated and selected from surface soils at this plain. These strains were identified based on cultural characteristics and chemotaxonomic analysis and classified in the genus Streptomyces. Growth of these strains was assured on a poor liquid medium containing NHAs as carbon and nitrogen sources and degradation occur only in the presence of gluco...

2010-01-01

467

Proximity effect correction of a laser lithography process for photomask fabrication

Proximity effect correction of a laser lithography process for photomask fabrication

We report on the improvement of Critical Dimension (CD) linearity on a photomask by applying the concept of process proximity correction to a laser lithographic process used for fabrication of photomasks. Rule-based Laser Process proximity Correction (LPC) was performed using an automated optical proximity correction tool and we obtained dramatic improvement of CD linearity on a photomask. A study on model-based LPC was executed using a two-Gaussian kernel function and we extracted model parameters for the laser lithographic process by fitting the model-predicted CD linearity data with measured ones. Model-predicted bias values of isolated space (I/S), Arrayed Contact (A/C) and Isolated Contact (I/C) were in good agreement with those obtained by the nonlinear curve-fitting method used for the rule-based LPC.

2004-07-01

468

Preliminary performance assessment for the Waste Isolation Pilot Plant, December 1992. Volume 1, Third comparison with 40 CFR 191, Subpart B

We report on the improvement of Critical Dimension (CD) linearity on a photomask by applying the concept of process proximity correction to a laser lithographic process used for fabrication of photomasks. Rule-based Laser Process proximity Correction (LPC) was performed using an automated optical proximity correction tool and we obtained dramatic improvement of CD linearity on a photomask. A study on model-based LPC was executed using a two-Gaussian kernel function and we extracted model parameters for the laser lithographic process by fitting the model-predicted CD linearity data with measured ones. Model-predicted bias values of isolated space (I/S), Arrayed Contact (A/C) and Isolated Contact (I/C) were in good agreement with those obtained by the nonlinear curve-fitting method used for the rule-based LPC.

2004-11-04

469

Phylogeography of rabies virus isolated from dogs in Brazil between 1985 and 2006

Before disposing of transuranic radioactive wastes in the Waste Isolation Pilot Plant (WIPP), the United States Department of Energy (DOE) must evaluate compliance with applicable long-term regulations of the United States Environmental Protection Agency (EPA). Sandia National Laboratories is conducting iterative performance assessments of the WIPP for the DOE to provide interim guidance while preparing for final compliance evaluations. This volume contains an overview of WIPP performance assessment and a preliminary comparison with the long-term requirements of the Environmental Radiation Protection Standards for Management and Disposal of Spent Nuclear Fuel, High-Level and Transuranic Radioactive Wastes (40 CFR 191, Subpart B).

1992-12-01

470

British Library Electronic Table of Contents (United Kingdom)

To establish the phylogeographic relationships in rabies viruses in Brazil, we studied a dataset retrieved from GenBank consisting of 71 genetic sequences from the coding region of the N gene of rabies viruses isolated in dogs over a period of 22?years. The Bayesian Markov chain Monte Carlo method available in the BEAST package was used with the GTR+G+?4 evolutionary model in conjunction with the relaxed uncorrelated lognormal molecular clock model and an exponential growth tree prior. A discrete phylogeographic diffusion model was also analyzed using a standard continuous-time Markov chain viewed with Google Earth to provide a spatial projection of the diffusion of genetic lineages based on their phylogeographic relationships. The topology of the time and substitution phylogenetic trees a...

2011-01-01

471

Oxidation of Elemental Sulfur by Fusarium solani Strain THIF01 Harboring Endobacterium Bradyrhizobium sp.

British Library Electronic Table of Contents (United Kingdom)

Nineteen fungal strains having an ability to oxidize elemental sulfur in mineral salts medium were isolated from deteriorated sandstones of Angkor monuments. These fungi formed clearing zone on agar medium supplemented with powder sulfur due to the dissolution of sulfur. Representative of the isolates, strain THIF01, was identified as Fusarium solani on the basis of morphological characteristics and phylogenetic analyses. PCR amplification targeting 16S rRNA gene and analyses of full 16S rRNA gene sequence indicated strain THIF01 harbors an endobacterium Bradyrhizobium sp.; however, involvement of the bacterium in the sulfur oxidation is still unclear. Strain THIF01 oxidized elemental sulfur to thiosulfate and then sulfate. Germination of the spores of strain THIF01 was observed in a liqui...

2010-01-01

472

Isolation and mapping of a polymorphic DNA sequence (pEFD64. 2) on chromosome 3 (D3S46)

Inhibitory activity of Pseudomonas sp. on Flavobacterium psychrophilum, in vitro

A 4.0 kb TaqI fragment of cosmid EFD64 isolated by a HBV-3 oligonucleotide was subcloned into AccI site of pUC18. MspI identifies 5 allelic VNTR polymorphism with bands between 2.6 and 4.6 kb. RsaI, TaqI, EcoRI, BamHI, HindIII and PvuII identify the same VNTR polymorphism. With RsaI, 80% heterozygosity were observed in 80 unrelated Caucasians. This RFLP pEFD64.2 has been assigned to chromosome 3 by linkage analysis with loci (APOD) known to span this chromosome. Co-dominant segregation for the RsaI RFLP was observed in 40 three generation families. RFLPs were observed under the normal hybridization and washing condition.

1988-10-11

473

British Library Electronic Table of Contents (United Kingdom)

Abstract A Pseudomonas sp. isolate MSB1 efficiently inhibited the growth of Flavobacterium psychrophilum of different serotypes on agar medium. A significant difference in the inhibition was observed between isolates of the less virulent FpT serotype compared to the Fd and Th serotypes. In broth coculture experiments, a low number of cells of MSB1 inhibited and outcompeted the F.psychrophilum cells. Also cell-free culture supernatant of MSB1 clearly repressed the growth of F.psychrophilum. A chromoazurol S assay suggested that MSB1 produced efficient siderophores, which most probably were responsible for the iron deficiency in the supernatant. The limited growth of F.psychrophilum in the supernatant was found to be partly because of the lack of available iron, but the results also indicate...

2011-01-01

474

Inhibition of heat shock protein 27 (HspB1) tumorigenic functions by peptide aptamers

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

2011-01-01

475

Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

British Library Electronic Table of Contents (United Kingdom)

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50??g/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30?min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

2011-01-01

476

Flavonols and an oxychromonol from Piliostigma reticulatum

British Library Electronic Table of Contents (United Kingdom)

The leaf extract from the plant Piliostigma reticulatum was found to exhibit antimicrobial activity against some bacteria and fungi such as Staphylococcus aureus (NCTC 6571), Escherichia coli (NCTC 10418), Bacillus subtilis (NCTC 8236), Proteus vulgaris (NCTC 4175), Aspergillus niger (ATCC 10578) and Candida albicans (ATCC 10231). Upon investigation of the chemical constituents present in the leaf extract, a total of seven compounds were isolated and their structures were unambiguously established by spectroscopic methods including HR-MS and NMR spectrometry. Four of the isolated compounds were novel, namely 6-C-methyl-2-p-hydroxyphenyloxychromonol (piliostigmol), 1, 6,8-di-C-methylquercetin-3,3prime,7-trimethyl ether, 2, 6,8-di-C-methylquercetin-3,3prime-dimethyl ether, 3 and 3prime,6,8,-...

2008-01-01

477

Extraction and analysis of antioxidant components from Origanum dictamnus

British Library Electronic Table of Contents (United Kingdom)

Three alternative extraction procedures were carried out in order to separate the antioxidant components and isolate an efficient extract from Origanum dictamnus. Procedure A included sequential extractions with petroleum ether (PE), diethyl ether (DE) and ethanol; procedure B sequential extractions with PE and ethyl acetate (EAc); procedure C a single step extraction with ethanol. The most efficient radical scavengers, according to the DPPH method, were isolated in ethanol extract of procedure A (mainly rosmarinic acid), followed by ethanol extract of procedure C. However, both ethanol extracts had low solubility in oil and could not protect it. EAc and DE extracts, containing mainly apigenin and epirosmanol ethyl ether, presented lower radical scavenging activity but were very effective ...

2007-01-01

478

Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics

British Library Electronic Table of Contents (United Kingdom)

Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm? MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.

2009-01-01

479

Direct patterning of complex oxides by pulsed laser deposition through stencils

The possibilities to grow isolated structures of complex oxides by pulsed laser deposition through stencils were investigated. A stencil consisting of a SiN membrane with apertures of several hundred nanometers embedded in a Si chip is placed in front of a heated substrate (up to 750 degrees Celsius). Deposition through these apertures results in resistless, direct patterning by local deposition of complex oxides like ferroelectric Lead Zirconate Titanate. The created isolated structures were analyzed by AFM imaging. Under-deposition, in this work called broadening, is inevitable during stencil deposition and is depending on deposition parameters, especially pressure. Different causes of broadening are mapped and discussed.

2007-04-01

480

Control rod devices

Cloning of the cDNA and gene for a human D sub 2 dopamine receptor

Purpose: To remove excessive driving pressure applied to an unisolated control rod drive by returning excessive coolant to a condensed water storage tank or to the inlet side of a drive water pump using a coolant flow rate control pipe of a control rod driving hydraulic system. Constitution: Excessive water is returned to a condensed water tank while controlling the excessive coolant by a flow control valve in response to variations in the pressure difference between the reactor pressure and the driving water line when the control rods are isolated using a pipe from the outlet side of the drive water pump to the condensed water storage tank. Thus, the control rod to be isolated is prevented form being dropped. (Sekiya, K.).

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Chemical constituents of Galianthe brasiliensis (Spreng.) E.L.Cabral and Bacigalupo (Rubiaceae); Constituintes quimicos de Galianthe brasiliensis (Rubiaceae)

A clone encoding a human D{sub 2} dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D{sub 2} receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

1989-12-01

482

Chemical constituents and anti-inflammatory and antioxidant activities evaluation of the leaves extracts of Chomelia obtusa Cham. and Schltdl. (Rubiaceae)

The paper describes the chemical constituents isolated from aerial parts of the plant Galianthe brasiliensis. From a methanol extract, the iridoid glycosides asperuloside, deacetylasperuloside, mixture of Z- and E-6-O-p-coumaroylscandoside methyl ester, the triterpene ursolic acid and the steroids stigmasterol, campesterol, b-sitosterol and 3-O-b-glucopyranosyl sitosterol were isolated. The structures of the natural products were identified on the basis of spectral data, including 2D NMR experiments. The antiproliferative properties of the crude methanolic extract were investigated against a series of nine human cancer cell lines. (author)

2006-05-15

483

Bioinformatic analysis of BBTV satellite DNA in Hainan

The phytochemical investigation of Chomelia obtusa leaves led to the isolation of four triterpenes (3-O-#beta#-D-quinovopyranosyl-28-O-#beta#-D-glycopyranosyl quinovic acid, 3-O-#beta#-D-quinovopyranosyl-28-O-#beta#-D-glycopyranosyl cincholic acid, and a mixture of ursolic and oleanolic acids), two flavonoids (3-O-#beta#-D-glycopyranosyl quercetin, 3-O-[#alpha#-L-rhamnopyranosyl-(1#->#6)-#beta#-D-galactopyranoside] quercetin), besides bornesitol and a mixture of 3,5- and 4,5-O-dicaffeoyl quinic acids. The structures of the isolated compounds were assigned on the basis of spectroscopic data, including two-dimensional NMR methods. The anti-inflammatory and antioxidant activities of the crude methanolic extract and of its fractions were evaluated. This is the first report on the chemical and biological investigation of the Chomelia genus. (author)

484

British Library Electronic Table of Contents (United Kingdom)

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiatio...

2011-01-01

485

Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)

British Library Electronic Table of Contents (United Kingdom)

Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess different protocols for the isolation of GALT cells from an important fish species in the Mediterranean aquaculture, the gilthead seabream. Mechanical, chemical and enzymatic treatments were assayed. Nylon wool columns and continuous density gradients were used for further separation of cell subpopulations. Light microscopy and flow cytometry showed that the highest density band (HD) consisted of a homogeneous lymphocytic population, whereas the intermediate density band (ID) corresponded to epithelial and secretory cells and some lymphocytes. Respiratory burst activity of total cell suspen...

2007-01-01

486

An early review of the performance assessment in the draft compliance certification application for the Waste Isolation Pilot Plant

Alcohol fermentation in olive oil extraction effluents

On March 31, 1995, the US Department of Energy filed a draft application for certification of compliance with the US Environmental Protection Agency (USEPA) to show the Waste Isolation Pilot Plant's compliance with the USEPA's environmental standards, making the application the first of its kind. Demonstration of compliance is by performance assessment. This paper is an early review of the performance assessment in the draft application, by the Environmental Evaluation Group, an oversight group. The performance assessment in the draft application is incomplete. Not all relevant scenarios have been analyzed. The calculation of potential consequences often does not use experimental data but rather estimates by workers developing additional data. The final compliance application, scheduled for October 1996 needs to consider additional scenarios, and be fully based on experimental data.

487

A study of the B 24 - A 19 transition of benzene molecule in different matrices

Eight culture-collection yeast strains of various species and five newly isolated stains were tested for both growth in olive oil extraction effluents and fermentation of the sugars in the same media. The culture-collection yeast strains did not grow in an effluent containing 2.86% sugar (w/v), 8 g/litre phenolic substances, 4.58 g/litre titratable acidity and pH 4.96, whereas the newly isolated strains of Torulopsis sp. MK-1, Saccharomyces norbensis MC-1, S. oleaceus MC-2 and S. oleaginosus grew well and fermented the sugars. In the medium mentioned above, they produced alcohol in amounts of 1.63 to 1.38%, respectively. None of the yeasts grew in an olive oil extraction effluent vacuum-concentrated to over 13-14% of dry matter. The strain of T. sp. MK-1 showed a higher stability.

1989-01-01

488

A polarized neutron reflectometry study of the spin glass freezing in a 29 nm thick AuFe film

Ultraveiolet spectroscopy of molecules in vapour phase gives valuable information about electronic structure of free molecules. But in many cases vaipour phase investigations are not possible and in order to isolate molecules within solid lattice, we used cryogenic temperature and high vacuum technology to study absorption spectrum within the spectral range (230-270)nm of an isolated benzene molecule in Argon, Krpton, Nitrogen, Carbon and methane matrices. The spectra shifts were measured and calculated in the matrix environment for the electronnic transition (B 24--A 19) in benzene molecule using the matrices mentioned above. Molar extinction coefficients and oscillator strength were measured too. (7 tabs., 32 figs., 50 refs.).

1988-01-01

489

A new approach to the isolation and characterization of wheat flour allergens

We performed polarized neutron reflectometry (PNR) experiments on a 29 nm thick Au{sub 93}Fe{sub 7} film in a temperature range from 295 K down to 2 K in a vertical magnetic field up to 6 T. These high-field experiments were performed on the C5 spectrometer in Chalk River, Canada, using a split-pair cryomagnet. The magnetization as determined by PNR can be described with a Brillouin function from 295 K down to 50 K assuming the magnetic moment of isolated Fe atoms, i.e. 4{mu}{sub B} per Fe atom. Below 50 K the onset of the spin-glass freezing is observed as a strong deviation from this Brillouin type behavior of isolated atoms.

2007-07-15

490

British Library Electronic Table of Contents (United Kingdom)

Summary Background The incidence of food allergy to wheat is increasing. Its diagnosis depends on the purity of major allergens and their inclusion in tests. Isolation and characterization of wheat allergens are therefore of utmost importance. Objective To purify and identify wheat flour allergens most frequently recognized by patients' IgE antibodies and to study their allergenicity. Methods Water/salt-soluble extracts from wheat flour were prepared and separated using a combination of ultrafiltration, isoelectric focusing and liquid chromatography. Purified proteins were analysed by immunoblotting using pooled sera from patients with atopic dermatitis who possessed IgE specific to wheat. Wheat proteins found to bind IgE were subsequently identified by matrix-assisted laser desorption/ion...

2011-01-01

491

A Comparative Biochemical Characterization of Microbial Transglutaminases: Commercial vs. a Newly Isolated Enzyme from Streptomyces Sp.

British Library Electronic Table of Contents (United Kingdom)

A new microbial transglutaminase (MTGase or MTG, EC 2.3.2.13) from a Streptomyces sp. strain isolated from Brazilian soil samples was characterized in crude and purified forms. The aim of this work is to provide relevant information about a new transglutaminase and to compare its characteristics with the well-known commercial transglutaminase from Ajinomoto Co. Inc. (Activa? TG-BP). The enzyme from Streptomyces sp., in both crude and pure forms, exhibited optimal activity in the 6.0?6.5 pH range and at 35?40?C. The results for the commercial enzyme were the same. A second maximum of activity was observed at pH?10.0 with both the crude Streptomyces sp. enzyme and the commercial enzyme. This interesting fact has not been reported in the literature previously. The fact that this second maximu...

2010-01-01

492

Vertical and Horizontal Variations in the Physiological Diversity of the Aerobic Chemoheterotrophic Bacterial Microflora in Deep Southeast Coastal Plain Subsurface Sediments

Resistance to powdery mildew in Spanish barley landraces is controlled by different sets of quantitative trait loci.

Aerobic chemoheterotrophic bacteria were isolated from surface soils and coastal plain subsurface (including deep aquifer) sediments (depths to 265 m) at a study site near Aiken, S.C., by plating on...Full Text Available

1989-05-01

493

Perlinhibin, a Cysteine-, Histidine-, and Arginine-Rich Miniprotein from Abalone (Haliotis laevigata) Nacre, Inhibits In Vitro Calcium Carbonate Crystallization

Twenty-two landrace-derived inbred lines from the Spanish Barley Core Collection (SBCC) were found to display high levels of resistance to a panel of 27 isolates of the fungus Blumeria graminis that exhibit a wide variety of virulences. Among these lines, SBCC145 showed high overall resistance and a distinctive spectrum of resistance compared with the other lines. Against this background, the main goal of the present work was to investigate the genetic basis underlying such resistance using a doubled haploid population derived from a cross between SBCC145 and the elite spring cultivar Beatrix. The population was genotyped with the 1,536-SNP Illumina GoldenGate Oligonucleotide Pool Assay (Barley OPA-1 or BOPA1 for short), whereas phenotypic analysis was performed using two B. graminis isolates. A major quantitative trait locus (QTL) for resistance to both isolates was identified on the long arm of chromosome 6H (6HL) and ...

2011-07-08

494

Overexpression of eIF-5A2 in mice causes accelerated organismal aging by increasing chromosome instability

We have isolated a 4.785 Da protein from the nacreous layer of the sea snail Haliotis laevigata (greenlip abalone) shell after demineralization with acetic acid. The sequence of 41...Full Text Available

2007-08-15

495

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.

BackgroundAmplification of 3q26 is one of the most frequent genetic alterations in many human malignancies. Recently, we isolated a novel oncogene eIF-5A2 within...Full Text Available

496

Effects of. gamma. -irradiation on isolated rat liver mitochondria

We have isolated the human prointerleukin 1 (proIL-1) beta gene from leukocyte and fetal liver libraries. The nucleotide sequence and its gene organization reveals that the proIL-1 beta gene is composed...Full Text Available

1986-10-24

497

Biological solubilization of coal in aqueous and nonaqueous media

Gamma-irradiation of isolated rat liver mitochondria with up to 475 Gy leading to hydrated electrons (G = 1.9, corrected for reaction with solutes), 30 Gy leading to carbohydrate radicals, (G = 5.6), 100 Gy leading to superoxide radicals (G = 6.2), and 130 Gy leading to formate radicals (G = 6.2) showed no effects on the rate of oxygen uptake in various respiratory states, the respiratory control ratio, or the adenosine diphosphate to atomic oxygen ratio. Typical values were 0.020-0.100 nmol O/sub 2/ s/sup -1/ mg protein/sup -1/ for State 1 respiration, 0.25-0.33 nmol O/sub 2/ s/sup -1/ mg protein/sup -1/ for State 4 respiration and 0.65-1.10 nmol O/sub 2/ s/sup -1/ mg protein/sup -1/ for State 3 respiration. Typical respiratory control ratios ranged from 2.0-3.5 for succinate and 4.0-6.5 for a 1:1 glutamate: malate substrate mixture. Adenosine diphosphate to atomic oxygen ratios with succinate as substrate varied from 1.6 to 1.9. In situ and in vitro irradiated ...

1987-01-01

498

Current investigations into the biological solubilization of coal with microorganisms focus on the production of solubilizing activity in fungi. Test organisms for this work include a species of the yeast Candida previously isolated from a lignite outcrop, and P. chrysosporium, a filamentous higher fungus which has played a major role in lignin biodegradation research. The studies described are primarily exploratory in nature, and are fundamental to the design of more sophisticated inquiries into the physiology of fungal coal solubilization.

1987-01-01

499

Battery side terminal

Alkane degradation under anoxic conditions by a nitrate-reducing bacterium with possible involvement of the electron acceptor in substrate activation

A side terminal battery is described comprising: a casing having a side wall made from a moldable material; and a plug formed of relatively deformable, electrically conductive, corrosion resistant material molded into the side wall to form a seal between the interior and exterior of the casing and including a battery terminal insert embedded therein so as to be exposed at the exterior of the casing and isolated, by the plug, from the interior of the casing.

1988-03-08

500