UK PubMed Central (United Kingdom)

A multispecialty panel of physicians evaluated a case series of 53 composite-materials workers in a large aircraft manufacturing facility who filed workers' compensation claims for illness labeled by...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Drawing on work in epistemology and the philosophy of science, this paper seeks to provide very general reasons for why a comparative perspective needs to be applied to the inferential procedures of research methodologies where these concern the issue of justifying knowledge claims. In particular, the paper explores the role of culture on a number of important patterns of reasoning that figure in inferential arguments in research methodologies. The patterns examined are induction, both enumerative and analytical, hypothetico-deductive reasoning, and abductive inference. In each case it is argued that substantive theories about the world, including cultures, significantly affect inferential procedures. Examples chosen to illustrate this in more detail mostly reflect the impact of Confucian ...

International Nuclear Information System (INIS)

The possible existence of preferential B-site disorder in A-15 compounds recently claimed on the basis of x-ray powder diffraction data but questioned from the viewpoint of known phase diagrams is examined for some Nb-based compounds of this type. It is concluded that x-ray powder data do not allow the determination of both order and compositional variables as suggested, and the latter must therefore be determined by some other method. (author).

Energy Technology Data Exchange (ETDEWEB)

A procedure for high level tritium labelling at the C2-H position of adenosine 5`-triphosphate ([2-{sup 3}H]-ATP, 1), based on the tritiodehalogenation reaction of 2-bromoadenosine 5`-triphosphate (2) has been elaborated. This precursor was prepared in a six-step synthesis from guanosine. The tritiodehalogenation of (2) for three hours over palladium oxide in phosphate buffer yielded tritium labelled ATP with high specific activity, in good chemical yield. (author).

CERN Document Server

Although good encryption functions are probabilistic, most symbolic models do not capture this aspect explicitly. A typical solution, recently used to prove the soundness of such models with respect to computational ones, is to explicitly represent the dependency of ciphertexts on random coins as labels. In order to make these label-based models useful, it seems natural to try to extend the underlying decision procedures and the implementation of existing tools. In this paper we put forth a more practical alternative based on the following soundness theorem. We prove that for a large class of security properties (that includes rather standard formulations for secrecy and authenticity properties), security of protocols in the simpler model implies security in the label-based model. Combined with the soundness result of (\\textbf{?}) our theorem enables the translation of security results in unlabeled ...

International Nuclear Information System (INIS)

Short note. The World Health Organization (WHO) has taken aim at critics of irradiated food, claiming that the process has the potential to reduce the incidence of foodborne diseases such as salmonellosis, to cut postharvest food losses and to provide a wider variety of foods for consumers. 'The unwarranted rejection of this process, often based on lack of understanding of what food irradiation entails, may hamper its use in those countries which may benefit most', Dr. Jean-Paul Jardel, WHO's assistant director-general, argued following a recent international conference on the subject. Critics, including Canadians, has opposed food irradiation for years, claiming that more needs to be known about its effects. WHO said the 'vast majority' of the 54 national delegations at the conference supported use of the technology on foods ranging from grain and potatoes to poultry, tropical fruit and strawberries. WHO wants governments ...

International Nuclear Information System (INIS)

Conflicting claims in the literature on the compatability of tachyons with general relativity are discussed. (author).

British Library Electronic Table of Contents (United Kingdom)

In 1946, the Philippines raised claims in the South China Sea over an area already known as Spratly Islands. This claim advanced through peculiar stages, starting when Thomas Cloma allegedly discovered islands in 1946, later named as Freedomland, and maturing to some extent in 1978 by the governments claim over the so-called Kalayaan Island Group. Considered as an oceanic expansion of its frontiers, this paper reviews the basis of the claim, first over the nature of Clomas activities, and secondly over the measures the Philippine government took as a reaction of Clomas claim of discovery of an area already known in western cartography as the Spratlys. Eventually, what is the nature of the link between the 1978 Kalayaan Islands Groups official claim and 1956 Clomas private one?

Energy Technology Data Exchange (ETDEWEB)

Bone formation and resorption have been measured in patients with idiopathic osteoporosis by histomorphometry of 7.5-mm trephine biopsies and in the whole body by 85Sr radiotracer methodology and calcium balances. The studies were synchronized and most were preceded by double in vivo tetracycline labeling. Correlations between histological and kinetic bone formation indices were better when better when based on the extent of double tetracycline labels than on measurements of osteoid by visible light microscopy. Correction of the kinetic data for long-term exchange, using 5 months' serial whole body counting of retained 85Sr, improved the fit of the kinetic to the histological data. A statistical analysis of the measurement uncertainties showed that the residual scatter in the best correlations (between exchange-corrected bone formation rates and double-labeled osteoid surface indices) could be ...

Wastenet

... Database listings you may be interested in: 3rd Party Reviewed Listings Sustainable Industrial Paper Strap - Natural & White High strength, sustainable, recyclable paper strap SDF Strapping, Inc. High Graphics Litho Lam Corrugated High Graphics Corrugated Packaging (Litho-Lam) Accurate Box Company, Inc. EcoSystem Packaging Solution Starch-based loosefill packing peanut system StarchTech GreenChoice 100 100% recycled, clay-coated paperboard Strathcona Paper, L.P. Greener Package Database! comprehensive sustainability data on packaging materials searchable by converted package or raw material standardizes reporting of sustainability claims many ...

CERN Document Server

One of the salient features of human perception is its invariance under dilatation in addition to the Euclidean group, but its non-invariance under special conformal transformation. We investigate a holographic approach to the information processing in image discrimination with this feature. We claim that a strongly coupled analogue of the statistical model proposed by Bialek and Zee can be holographically realized in scale invariant but non-conformal Euclidean geometries. We identify the Bayesian probability distribution of our generalized Bialek-Zee model with the GKPW partition function of the dual gravitational system. We provide a concrete example of the geometric configuration based on a vector condensation model coupled with the Euclidean Einstein-Hilbert action. From the proposed geometry, we study sample correlation functions to compute the Bayesian probability distribution.

Energy Technology Data Exchange (ETDEWEB)

This paper describes the design and operating experience with a high performance control system tailored to the requirements of the SuperHILAC accelerator. A large number (20) of the latest 16-bit microcomputer boards are used in a parallel-distributed manner to get a high system bandwidth. Because of the high bandwidth, software costs and complexity are significantly reduced. The system by its very nature and design is easily upgraded and repaired. Dynamically assigned and labeled knobs, together with touch-panels, allow a flexible and efficient operator interface. An X-Y vector graphics system provides for display and labeling of real-time signals as well as general plotting functions. This control system allows attachment of a powerful auxiliary computer for scientific processing with access to accelerator parameters.

Energy Technology Data Exchange (ETDEWEB)

The radioiodine-labeled amines currently available as brain-imaging agents, based on our previous work and that of others, are prepared either by exchange labeling or by direct iodination of a protected intermediate. The intrinsic slowness of these processes limits their potential for use with the positron-emitting 122I, as it has a half-life of only 3.6 min. This isotope has advantages of a low dose to the patient and availability from a generator containing the parent 20-h 122Xe. To develop a radiopharmaceutical in which 122I could be utilized, we prepared a number of secondary and tertiary amines (maintaining the 2,5-dimethoxy substitution pattern which allows direct iodination at the 4-position) with 131I. The organ distributions of these compounds were studied, and the best properties were found in the N,N-dimethyl homologue (2,5-dimethoxy-N,N-dimethyl-4-iodoamphetamine). This compound was successfully synthesized in a ...

Science.gov (United States)

Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data set requires the numerical solution of a nonlinear inverse problem. To this end, a general algorithm for flux estimation from metabolic carbon labelling experiments based on the least squares approach is developed in this contribution and complemented by appropriate tools for statistical analysis. The linearization technique usually applied for the computation of nonlinear confidence regions is shown to be inappropriate in the case of large exchange fluxes. For this reason a sophisticated compactification transformation technique for nonlinear statistical analysis is developed. Statistical analysis is then ...

Wastenet

Labeling of organic res- taurants, cantinas, etc.

Energy Technology Data Exchange (ETDEWEB)

The oxidative dehydrogenation (ODH) of light alkanes is an alternative way for the production of olefins. A wide variety of catalytic systems has been investigated. Vanadium oxide based catalysts were described in the literature as effective catalysts for the ODH of propane. The catalytic activity and selectivity depend on the kind of support material, the kind of dopants and the formation of complex metal oxide phases. In recent papers it was claimed that both orthovanadate and/or pyrovanadate species are selective for the ODH of propane. Niobia based materials were investigated as catalysts for acidic and selective oxidation type reactions. In the ODH of propane niobia exhibited a high selectivity to propene but the conversion of propane was low. V{sub 2}O{sub 5}-Nb{sub 2}O{sub 5} catalysts proved to be catalytically active and selective and showed no formation of oxygenates. In the present study the influence of the ...

CERN Document Server

CERN earns "Nature & Economie" label for green land management

Science.gov (United States)

... Final accounting for extended to include ?mounts applicable ... is verified by the finance or disbursing ... the contractor must claim DLA, contractors must ...

CERN Document Server

A risk-neutral method is always used to price and hedge contingent claims in complete market, but another method based on utility maximization or risk minimization is wildly used in more general case. One can find all kinds of special risk measure in literature. In this paper, instead of using market modified risk measure, we use a kind of risk measure induced by g_\\Gamma-solution or the minimal solution of a Constrained Backward Stochastic Differential Equation (CBSDE) directly when constraints on wealth and portfolio process comes to our consideration. Such g_\\Gamma-solution and the risk measure generated by it is well defined on appropriate space under suitable conditions. We adopt the inf-convolution of convex risk measures to solve some optimization problem. A dynamic version risk measures defined through g_\\Gamma-solution and some similar results about optimal problem can be got in our new framework and by our new approach.

Science.gov (United States)

The financial evaluation of capital and operating cost estimates for a dry coke quenching facility installation indicates a marginal return on investment if the evaluation is based exclusively on steam credits. However, the potential improvements in environmental emissions and reported, but unverified, blast furnace operations may justify the expenditure. The Russian and Japanese claim of 2 to 4% reduction in blast furnace coke usage with up to 4% increase in iron production has not been demonstrated in the domestic steel industry and, therefore, is not included in the financial analysis. A 2% coke reduction represents a savings of approximately $0.81 per ton of hot metal produced or an additional annual credit of $1,800,000 for dry coke quenching, exclusive of productivity gains. Additional credit of this magnitude would increase the estimated discounted cash flow return from 10% to approximately 16% for the estimated capital expenditure of ...

Energy Technology Data Exchange (ETDEWEB)

Claims of property value loss are commonly raised by homeowners when noxious facilities are sited or when new information about the hazards of existing facilities is made public. While the capitalization of externalities into land values is consistent with economic theory, empirical measurement of impacts has not generated consistent results. This is true both for hedonic measurements as well as other types of econometric analyses. While it is well established that job and site risks have similar impacts on regional labor markets, there are no studies relating the presence of a broad range of noxious facilities to local wage premiums. In contrast, this study employs an interregional framework in a hedonic analysis of both wage and property markets and considers eight different facility classifications. This paper discusses the development of the hedonic model employed in this study. It develops more fully the theoretical advantages of the intercity model and ...

CERN Document Server

The profusion of online digital images presents new challenges for image indexing. Images have always been problematic to describe and catalogue due to lack of inherent textual data and ambiguity of meaning. An alternative to time-consuming professionally-applied metadata has been sought in the form of tags, simple keywords that form a flat structure known as distributed classification, or more popularly as a folksonomy. This research aims to increase understanding of why people tag and how effective they find it for searching, using as the focus. Open-ended questionnaires were sent out to members of the photo-sharing website Flickr, with the opportunity to post comments to an online discussion space. There is also a systematic comparison between a tag-based system and a more traditional controlled vocabulary, to test out the claims made regarding the suitability of tagging for searching and browsing. For this purpose Flickr has been compared ...

Energy Technology Data Exchange (ETDEWEB)

An electrochemical detection method for analyzing sequence-specific DNA using gold nanoparticle DNA probes and subsequent signal amplification step by silver enhancement is described. The assay relies on the electrostatic adsorption of target oligonucleotides onto the sensing surface of the glassy carbon electrode (GCE) and its hybridization to the gold nanoparticle-labeled oligonucleotides DNA probe. After silver deposition onto gold nanoparticles, binding events between probe and target were monitored by the differential pulse voltammetry (DPV) signal of the large number of silver atoms anchored on the hybrids at the electrode surface. The signal intensity difference permits to distinguish between the match of two perfectly matched DNA strands and the near-perfect match where just one base pair was wrong. Coupled with this 'nanoparticle-promoted' reduction of silver signal amplification method, the sensitivity of this ...

Energy Technology Data Exchange (ETDEWEB)

Electric cars have long been seen as science fiction. This may be changing. As the 1990s began, several companies chose to launch publicity campaigns for electric vehicles. In California, experimental programmes are gaining momentum, in the context of LA's appalling air pollution problems and tightening clean air standards. Supporters claim that electric motive power is a key to solving several environmental problems involving atmospheric pollution. These claims are examined. (author).

Energy Technology Data Exchange (ETDEWEB)

Previous work showed that (TH)p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the peptidyltransferase center. The ...

Energy Technology Data Exchange (ETDEWEB)

This study was carried out to evaluate the biochemical and morphological effects of ionizing radiation on ovary. Immature mice (ICR, 3 week-old) were irradiated at a dose of LD{sub 80(30)} at KAERI. The ovaries were collected after 6 hours, 12 hours, 1 day, and 2 days post irradiation. To analyze the morphological changes, histological staining with hematoxylin-eosin, immuno- histochemical preparation using in situ 3'-end labeling was performed. DNA fragmentation analysis and flowcytometric evaluation of DNA extracted from whole ovary were performed. As a result of DNA fragmentation analysis, DNA fragments with 185, 370, and 555 base pairs were clearly shown at 6 hours post irradiation. The percentage of A{sub 0} cell cycle was significantly increased in the irradiated group than control. In situ 3'-end labeled follicles were increased at 6 hours post irradiation. The radiation-induced follicular atresia ...

British Library Electronic Table of Contents (United Kingdom)

A stable BSA blocking poly(dimethylsiloxane) (PDMS) microchannel was prepared based on in situ synthesized PDMS-gold nanoparticles composite films. The modified microchip could successfully suppress protein adsorption. The assembly was followed by contact angle, charge-coupled device (CCD) imaging, electroosmotic flow (EOF) measurements and electrophoretic separation methods. Contact angle measurements revealed the coated surface was hydrophilic, water contact angle for coated chips was 45.2^o compared to a water contact angle for native PDMS chips of 88.5^o. The coated microchips exhibited reproducible and stable EOF behavior. With FITC-labeled myoglobin incubation in the coated channel, no fluorescence was observed with CCD image, and the protein exhibited good electrophoretic effect in ...

British Library Electronic Table of Contents (United Kingdom)

To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were labeled with PKH26, and cell morphology was observed under microscope. Cell cycle, apoptotic cell death, expression of PKH26, and the proliferation rate were evaluated. Additionally, fluorescence intensity of PKH26 labeling at different passage times was quantified. There were no detectable differences in cell morphology, cell growth, and proliferation rate after PKH26 labeling. In addition, fluorescence intensity of PKH26 labeling was gradually reduced with increase of the passage times. The PKH26 labeling disappeared after passage six times. In summary, PKH26 labeling is a safe and ef...

Energy Technology Data Exchange (ETDEWEB)

Following the United Kingdom's nuclear tests at Maralinga in Australia in the 1960's, the Australian government signed away its rights to claim compensation for decontamination costs in a 1968 agreement. Controversy now rages as the Australian government is claiming pound33 million for this purpose from the United Kingdom to compensate the Maralinga Tjarutja for the loss of access to their traditional lands. They claim that the UK clean-up operation was inadequate and that much plutonium was spread over the land, rather than contained in pits. Measurements of contamination at the site were wrong by a factor of ten according to a 1984 visit when Australian scientists were horrified to realize that an Aboriginal child living near Taranaki could inhale more than 460 millisieverts a year. As ministers from both countries meet in June 1993 the debate is likely to be heated and hotly defended. (UK).

Energy Technology Data Exchange (ETDEWEB)

Syntheses are described for tetra and pentadeutero indole-3-acetic acid (IAA) labeled in positions 4,5,6,7 or 2,4,5,6,7 of the indole moiety. Polydeuterated IAA is proposed as an internal standard for gas chromatographic-mass spectrometric analysis of IAA by selected ion monitoring. Nanogram amounts of IAA may be assayed by monitoring the base peak of IAA at m/z = 130 (134 for d/sub 4/-IAA) and the molecular ion of the methyl ester of IAA at 189 (193 for d/sub 4/-IAA). Deuterium in positions 4,5,6, and 7 and, to only a slightly lesser extent, that in position 2 of IAA is retained during alkali treatment, thus permitting use of these compounds as internal standards for assay of IAA released by alkaline hydrolysis of ester and amide conjugates. The use of polydeutero internal standards separates the standards from the isotope cluster caused by the normal abundance of heavy isotopes and also permits use of reduced mass resolution, thus leading to ...

International Nuclear Information System (INIS)

... biological variability body distribution kidneys labelled compounds lead 203

International Nuclear Information System (INIS)

In the present study the authors have shown that glycoproteins are present in the plasma membrane of Acanthamoeba castellanii by utilizing different radioactive labeling techniques. Plasma membrane proteins in the amoeba were iodinated by "1"2"5I-lactoperoxidase labeling and the solubilized radiolabeled glycoproteins were separated by lectin-Sepharose affinity chromatography followed by polyacrylamide gel electrophoresis. The periodate/NaB"3H_4 and galactose oxidase/NaB"3H_4 labeling techniques were used for labeling of surface carbohydrates in the amoeba. Several surface-labeled glycoproteins were observed in addition to a diffusely labeled region with M_r of 55,000-75,000 seen on electrophoresis, which could represent glycolipids. The presence of glycoproteins in the plasma membrane of Acanthamoeba castellanii was confirmed by metabolic ...

ScienceCinema

...same sort of handle ? religious that we have on global warming or energy policy or ...other words my claim is that we need to study religion the way we study global warming and all the other really serious phenomena on this planet ...

Energy Technology Data Exchange (ETDEWEB)

An ex-employee's claims that inadequate enforcement of radiation safety regulations allowed excess radiation exposure thereby causing his deteriorating health was not substantiated by a thorough investigation.

Science.gov (United States)

Methods of acidizing a subterranean well formation, acidizing compositions, and sequestering additives for preventing the precipitation of metal compounds in the formation are described. Use of the methods and chemicals described should lead to improved formation permeability as compared to use of previous methods and chemicals. 12 claims.

CERN Document Server

This paper is about two arrangements of hyperplanes. The first --- the Shi arrangement --- was introduced by Jian-Yi Shi to describe the Kazhdan-Lusztig cells in the affine Weyl group of type $A$. The second --- the Ish arrangement --- was recently defined by the first author who used the two arrangements together to give a new interpretation of the $q,t$-Catalan numbers of Garsia and Haiman. In the present paper we will define a mysterious "combinatorial symmetry" between the two arrangements and show that this symmetry preserves a great deal of information. For example, the Shi and Ish arrangements share the same characteristic polynomial, the same numbers of regions, bounded regions, dominant regions, regions with $c$ "ceilings" and $d$ "degrees of freedom", etc. Moreover, all of these results hold in the greater generality of "deleted" Shi and Ish arrangements corresponding to an arbitrary subgraph of the complete graph. Our proofs are based on nice ...

DEFF Research Database (Denmark)

The determination of conformational preferences in unfolded and disordered proteins is an important challenge in structural biology. We here describe an algorithm to optimize energy functions for the simulation of unfolded proteins. The procedure is based on the maximum likelihood principle and employs a fast and efficient gradient descent method to find the set of parameters of the energy function that best explain the experimental data. We first validate the method by using synthetic reference data, and subsequently apply the algorithms to data from nuclear magnetic resonance spin-labeling experiments on the Delta 131 Delta fragment of Staphylococcal nuclease. A significant strength of the procedure that we present is that it directly uses experimental data to optimize the energy parameters, without relying on the availability of high resolution structures. The procedure is fully general and can be applied to a range of experimental data and ...

British Library Electronic Table of Contents (United Kingdom)

Recent legislative changes in both Europe and the USA have increased the responsibility of drug developers to purposefully study the agents they market in children so that specific dosing recommendations can be made to assist clinicians in their use. Typically, clinicians use empirical- or experiential-based rationales for selecting the dose to use in children, generally in a manner that attempts to achieve the same dose-exposure or pharmacokinetic profile in children as in adults. However, whether this approach achieves the necessary dose exposure or exposure effect needed may not be systematically explored during off-label use. This creates the opportunity for under- or over-exposure in children, particularly in very young children (i.e., less than 2 years old) where a combination of fac...

International Nuclear Information System (INIS)

Values of the chemical yield, G, and of the molar extinction coefficient, epsilon, for Fricke dosimetry, have been recommended by ICRU. For epsilon, the value in ICRU Reports 17 and 21, 2205 1.mol"-"1.cm"-"1, based on an analysis of 83 reported values. It was suggested that, where neither a careful wavelength calibration nor absorbance linearity checks had been performed, a standard error of 1.5% should be assumed; otherwise 0.15% could be accepted. Alternatively, the accuracy in applying this chemical dose meter was considered improved if there was an independent determination of epsilon with the same spectrophotometer as was used for the determination of the optical density of irradiated Fricke solution. The accuracy claimed for both methods remains questionable, since no complete argumentation could be given for the differences of up to 13% in the published values. Taking into account the discussion of the sources of error by Bryant, ...

Energy Technology Data Exchange (ETDEWEB)

We report a simple method for the label-free detection of double-stranded DNA using surface-enhanced Raman scattering (SERS). We prepared cetyltrimethylammonium bromide (CTAB)-capped silver nanoparticles and a DNA-nanoparticle complex by adding silver nanoparticles to {lambda}-DNA solutions. In the present study, the utilization of CTAB-capped silver nanoparticles facilitates the electrostatic interaction between DNA molecules and silver nanoparticles; at the same time, the introduction of DNA avoids adding aggregating agent for the formation of nanoparticle aggregates to obtain large enhancement of DNA, because the DNA acts as both the probe molecules and aggregating agent of Ag nanoparticles. Atomic force microscopy (AFM) studies show that the morphology of DNA-Ag nanoparticle complexes seems to be determined by the concentrations of the DNA and the nanoparticles. Surface-enhanced Raman scattering (SERS) studies show that the morphology of the complexes plays a ...

UK PubMed Central (United Kingdom)

Three photaffinity labeled derivatives of epothilone D were prepared by total synthesis, using efficient novel asymmetric synthesis methods for the preparation of two important synthetic building...Full Text Available

UK PubMed Central (United Kingdom)

Rat somatotropin (growth hormone) was labelled biosynthetically by incubating anterior pituitary lobes with radioactive amino acids for 24 h in a simple buffered salts medium containing glucose. The...Full Text Available

UK PubMed Central (United Kingdom)

Objective:In this article, we describe a successful low-dose, off-label usage of drotrecogin alfa (Xigris), given at 18 mcg/kg per hour, in a patient with severe sepsis who had an...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundObesity is a major public health threat and policies aimed at curbing this epidemic are emerging. National calorie labeling legislation is forthcoming and requires rigorous...Full Text Available

International Nuclear Information System (INIS)

The basic approach to radioactive labeling techniques in hemoglobin identification consists of mixing 14C labeled normal carrier globin with 3H labeled unknown globin and carrying out the subsequent separations and purifications looking at the distribution of 3H and 14C radioactivity. This technique was devised to ascertain the amino acid substitution in Hb Indianapolis and was instrumental in solving the structure of Hb Vicksburg. It was also useful in determining the purity of gamma-chains from cultures of human and baboon erythroid progenitors. Another use of this technique would be in identifying aberrant peaks of radioactivity in chain separation chromatograms, such as ''pre-peaks''.

International Nuclear Information System (INIS)

Astatine conversed into cationic form is shown to form stable complex with diethylenetriaminpentacetic acid. Due to this complex astatine joins RN_2 type monoclonal antibodies. More favorable conditions to prepare astatine labelled antibodies are found. Chromatographical analysis and electromigration experiments have shown that astatine is strongly retained in in-vitro biomolecule. Astatine did not escape from the labelled antibodies even in case of urea effect on them. Immune activity of astatine labelled antibodies remained similar in 20 h. 28 refs., 4 figs.

Energy Technology Data Exchange (ETDEWEB)

Short communication.

Energy Technology Data Exchange (ETDEWEB)

A useful method for preparing radioiodinated photoaffinity labels from alkyl anilines which offer significant advantages over present methods is described. The one-pot synthesis gives good radiochemical yields (40-64%) of pure, high specific activity (350-1500 mCi/{mu}mol) {sup 124}I labelled iodaryl azides while minimising manipulation of radioactive materials. Purification of the ({sup 125}I)iodoazido photoaffinity labels is achieved by high performance liquid chromatography. (author).

Energy Technology Data Exchange (ETDEWEB)

A {lambda}gt11 cDNA library was constructed from poly(U)-Spharose-selected Entamoeba histolytica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit polyclonal anti-E. histolytica serum. A 700-base-pair cDNA insert was isolated and the nucleotide sequence was determined. The deduced amino acid sequence of the cDNA revealed a cysteine-rich protein. DNA hybridizations showed that the gene was specific to E. histolytica since the cDNA probe reacted with DNA from four axenic strains of E. histolytica but did not react with DNA from Entamoeba invadens, Acanthamoeba castellanii, or Trichomonas vaginalis. The insert was subcloned into the expression vector pGEX-1 and the protein was expressed as a fusion with the C terminus of glutathione S-transferase. Purified fusion protein was used to generate 22 monoclonal antibodies (mAbs) and a mouse polyclonal antiserum specific for the E. histolytica portion of the fusion protein. ...

Energy Technology Data Exchange (ETDEWEB)

In previous work the authors showed that on photolysis of Escherichia coli ribosomes in the presence of ({sup 3}H)tetracycline (TC) the major protein labeled is S7, and they presented strong evidence that such labeling takes place from a high-affinity site related to the inhibitory action of TC. In this work they use single protein omission reconstitution (SPORE) experiments to identify those proteins that are important for high-affinity TC binding to the 30S subunit, as measured by both cosedimentation and filter binding assays. With respect to both sedimentation coefficients and relative Phe-tRNA{sup Phe} binding, the properties of the SPORE particles they obtain parallel very closely those measured earlier, with the exception of the SPORE particle lacking S13. A total of five proteins, S3, S7, S8, S14, and S19, are shown to be important for TC binding, with the largest effects seen on omission of proteins S7 and S14. Determination of the ...

International Nuclear Information System (INIS)

The aim of this paper was to develop potential technetium 99m-labeled diagnostic imaging agents specific for the detection of A#beta# plaques. Based on previously obtained A#beta# plaque-specific biphenyls containing a benzimidazol group, "9"9Tc"m and Re-benzimidazol derivatives, ["9"9Tc"m(CO)_3]"+-EPBI and Re(CO)_3 Cl (EPBI), were prepared. The latter showed binding affinities towards A#beta#_(_1_#approx#_4_0_) aggregates in vitro (K_d=13.3 #mu#mol/L) by fluorophotometry. 2-(1-Ethylbenzimidazol-2-yl) pyridine (EPBI) and Re(CO)_3 Cl (EPBI) were synthesized. Binding affinity of Re(CO)_3 Cl (EPBI) for A#beta#_(_1_#approx#__4_0_) aggregates was determined. ["9"9Tc"m(CO)_3]"+-EPBI was prepared and analyzed by HPLC and paper eletrophoresis. Its biodistribution in mice was obtained. The K_d value of Re (CO)_3 Cl (EPBI) is 13.3 #mu#mol/L. Biodistribution of ["9"9Tc"m(CO)_3]"+-EPBI in mice shows brain penetration (0.63 #+-# 0.17) %ID/g (n=3) at 2 min ...

Energy Technology Data Exchange (ETDEWEB)

Carbohydrate and nucleotide structural determination using modern spectroscopic techniques is dependent on our ability to label oligonucleotides and oligosaccharides with stable isotopes. Uniform Carbon 13 and Nitrogen 15 labeling of oligonucleotides is important to present-day efforts, which are focused on determining the structure of relatively small oligosaccharides and oligonucleotides, which form the elements of larger structures. Because of the relatively recent interest in three-dimensional structure, the development of techniques used to label them has lagged behind parallel techniques used to label peptides and proteins. Therefore, this group`s discussion focused primarily on problems faced today in obtaining oligonucleotides labeled uniformly with carbon 13 and nitrogen 15.

Energy Technology Data Exchange (ETDEWEB)

Laboratory {open_quotes}jar{close_quotes} tests compared eleven different fuel oil and diesel fuel sludge-control additives. Factors studied included (1) ability to disperse and prevent buildup of sludge deposits on surfaces, (2) ability to protect steel from corrosion, (3) ability to inhibit growth and proliferation of bacteria, and (4) ability to disperse water. Results varied greatly, and it was found that many commercial products do not do what they claim. It is concluded that fuel retailers should not believe manufacturers` claims for their additive products, but rather should test such products themselves to be sure that the benefits of treatment are real. A simplified form of the procedure used here is proposed as one way for dealers to do such testing.

Energy Technology Data Exchange (ETDEWEB)

W have introduced template-synthesized metal phosphate nanoparticle labels for electrochemical immunoassay. Such use of an apoferritin template offers a simple and convenient route to prepare metallic nanoparticle labels for electrochemical immunoassays and avoid the complicated and time-consuming nanoparticle synthesis process (QD synthesis). Releasing metal ions from metal phosphate in an acetate buffer (pH 4.6) eliminates the harsh condition in the traditional metallic nanoparticle dissolution (e.g., strong acid dissolution of QDs and gold nanoparticles). This method is ultrasensitive and its DL is low to 77fM. The simultaneous detection of multiple protein targets is easily performed by using different metal phosphate nanoparticle labels (cadmium phosphate and lead phosphate). This approach can be extended to prepare multiple metal (such as zinc, lead, cadmium, copper, indium, gold, silver) phosphate nanoparticle ...

Energy Technology Data Exchange (ETDEWEB)

Fourier transform infrared (FTIR) difference spectra have been obtained for the bR----K, bR----L, and bR----M photoreactions in bacteriorhodopsin mutants in which Asp residues 85, 96, 115, and 212 have been replaced by Asn and by Glu. Difference peaks that had previously been attributed to Asp COOH groups on the basis of isotopic labeling were absent or shifted in these mutants. In general, each COOH peak was affected strongly by mutation at only one of the four residues. Thus, it was possible to assign each peak tentatively to a particular Asp. From these assignments, a model for the proton-pumping mechanism of bR is derived, which features proton transfers among Asp-85, -96, and -212, the chromophore Schiff base, and other ionizable groups within the protein. The model can explain the observed COOH peaks in the FTIR difference spectra of bR photointermediates and could also account for other recent results on site-directed mutants of bR.

Energy Technology Data Exchange (ETDEWEB)

The {sup 176}Ybn,{gamma}{sup 177}Yb-{beta}{sup -}{yields}{sup 177}Lu process was investigated to provide no-carrier-added (nca) {sup 177}Lu. The radiochemical separation of the {sup 177}Lu from the macro-amounts of the ytterbium target based on the cementation process, i.e. the selective extraction of Yb by Na(Hg) amalgam from Cl{sup -}/CH{sub 3}COO{sup -} electrolytes, followed by a final cation exchange purification. The cementation separation process provides a decontamination factor of Yb(III) of 10{sup 4}, the cation exchange purification adding a decontamination factor of >10{sup 2}. The nca {sup 177}Lu is available in radiochemically pure form despite the chemical similarity of the lanthanides with 75{+-}5% overall separation yield within 4-5 h. It can be used to synthesise nca {sup 177}Lu labelled radiotherapeuticals.

Scientific Electronic Library Online (English)

Abstract in english The tegument surface of the adult schistosome, bounded by a normal plasma membrane overlain by a secreted membranocalyx, holds the key to understanding how schistosomes evade host immune responses. Recent advances in mass spectrometry (MS), and the sequencing of the Schistosoma mansoni transcriptome/genome, have facilitated schistosome proteomics. We detached the tegument from the worm body and enriched its surface membranes by differential extraction, before subjecting t (more) he preparation to liquid chromatography-based proteomics to identify its constituents. The most exposed proteins on live worms were labelled with impearmeant biotinylation reagents, and we also developed methods to isolate the membranocalyx for analysis. We identified transporters for sugars, amino acids, inorganic ions and water, which confirm the importance of the tegument plasma membrane in nutrient acquisition and solute balance. Enzymes, ...

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A magnetic tape consisting of oil-shale Fischer assay data for selected well cores in the Piceance Creek Basin was prepared as a supplement to NTIS tape PB-230 607/AS. Both tapes serve as a data base for calculating oil-shale resources (Pitman, 1981). The tape is in standard IBM format and has the following specifications: EBCDIC (IBM standard), card-image, blocksize 1720 (blocked 20 to 1), record length 86, odd parity, 1,600 bpi, 9-track, no label tape. Each data file on the tape consists of records containing oil-shale analyses for one well core. The first record in a data file consists of a well-core identifier which is a three-digit number preceded by the character 'C'. The second record contains the well name and location, and the remaining records contain Fischer assay data.

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Sheep red blood cells (SRBCs) were labelled with a concanavalin A-luminol-bovine serum albumin conjugate specific for the transmembrane anion transport protein (Band 3) and exposed to 2450-MHz continuous-wave microwave radiation at an average specific absorption rate of 91W/kg for 10 min. The temperature was held constant at 25, 37, 40, 42, or 45C with an airflow heat-exchange system. Following exposure to microwave or air heating, the decrease in residual base-activated chemiluminescence (CL) of the SRBCs was measured as an indication of infield oxidase activity. Air heating resulted in a significant decrease in residual CL at temperatures above 37C (74% decrease at 45C). Microwave radiation inhibited the decline in residual CL above 37C. At 45C the inhibition was 40%. The results suggest microwave radiation either reversibly altered the thermodynamics of oxygen binding to haemoglobin or failed to energize a significant portion of the ...

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A procedure for the determination of carboxylic acids (benzoic and C{sub 8}-C{sub 16} aliphatic acids) in oxidised engine oils is described. It is based on a simple and selective derivatisation of the acids with a fluorescent label 9-anthracene-methanol followed by reverse-phase separation on an ODS column with a water-acetonitrile-tetrahydrofuran (20:40:40 by volume) mobile phase and post-column reaction with bis(2,4-dinitrophenyl) oxalate and hydrogen peroxide. The chemiluminiscence emission from the reaction was monitored using a lamina flow-through detector. Analysis of oxidised oils showed that although there was quenching of chemiluminiscence emission by the oil matrix, the acids could be determined over the range 0-0.4% (m/v) in the oil with a detection limit of 0.013% (m/v) (130 pmol on-column with a 20 {mu}l injection) for lauric acid. 8 figs., 2 tabs., 17 refs.

British Library Electronic Table of Contents (United Kingdom)

A new automated synthesis procedure of 1-H-1-(3-[18F]fluoro-2-hydroxypropyl)-2-nitroimidazole ([18F]FMISO), a specific hypoxia imaging agent with great significances for the noninvasive, dynamic hypoxia evaluation of cancer, was developed by modifying Explora FDG4 module, a commercial [18F]FDG production system, in this study. Its radiochemical synthesis was carried out via two sequent reaction steps, i.e. the nucleophilic displacement of labeling precursor 1-(2?-nitro-1?-imidazolyl)-2-O-tetrahydropyranyl-3-O-tosyl-propaned iol (NITTP) with activated 18F- ion at 100 ?C for 8 minutes, and the following hydrolysis with 1M HCl at 100 ?C for 5 minutes and neutralization with 1M NaOH. Two-pot reaction with two independent separations was adopted to assure the good separation of final product vi...

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Intact rat fat cell exposed to 12.5 ..mu..M (..gamma..-32P)ATP incorporate label into specific proteins within minutes. By solubilizing the reaction mixture with SDS which bypasses the subcellular fractionation steps, the labeled proteins can be identified in autoradiographs of SDS-PAGE gels. The most prominently labeled protein has an M/sub r/ of 42,000. Localization of this component to the cell surface can be made on the basis of inhibition of phosphorylation by addition of a protein derived from the rat brain with protein kinase inhibitory property, susceptibility of the phosphorylated protein to the tryptic digestion, inhibition of phosphorylation of this protein after brief exposure to melittin. To rule out the possibility that the cell surface protein might be a mitochondrial contaminant from broken cells, /sup 32/Pi-labeled and (..gamma..-/sup 32/P)ATP-labeled cells were ...

Energy Technology Data Exchange (ETDEWEB)

A process is claimed for stabilizing fuels during storage by blending therewith oxalic acid derivatives of mono- or bis-succinimides of polyalkylpolyamines. The fuels are stabilized by the presence of 10 to 100 parts per thousand barrels of these oxamides. For particular applications mixtures of monoand bis-succinimides in varying proportions can be used.

International Nuclear Information System (INIS)

A patent is claimed for the invention of a hardening (ionizing radiation resistance) process for MOS type components and CMOS or bipolar type components. The ionizing radiation effect on those systems is the electron-hole pair production, which induces interference phenomena. The MOS main structure is successively composed of a silicon substrate layer, a layer of an irradiation resistant material and a layer of partially monocrystalline silicon.

British Library Electronic Table of Contents (United Kingdom)

Significant recent writings have suggested strong parallels and actual links between the historical legal architectures of Canadian Aboriginal policy and South African apartheid. This article's further probing of these claims shows them to be very doubtful. At the same time, some more limited legal parallels between the two systems shows the importance of undertaking further analysis of the transmission and exchange of legal ideas and norms within the Commonwealth order.

International Nuclear Information System (INIS)

A photoaffinity derivative of the local anesthetic trimethisoquin, 5-azido-["3H]trimethisoquin (5-A["3H]T) labelled bands of app. mol. wt 50 k and 66 k. To explain the rather paradoxical labelling by 5-A["3H]T of two polypeptide chains instead of one, three possibilities were considered: (i) The site for non-competititve blockers is not carried by the 50 k or 66 k chain; however, these chains lie in the vicinity of the binding site and become preferentially labelled since they present chemical groups with which the nitrene group of 5-A["3H]T reacts. (ii) The 50 k and 66 k chains are different but carry binding sites for non-competitive blockers with similar reactivities. (iii) The 50 k chain labelled by 5-A["3H]T derives from the 66 k chain by proteolysis. These results show that the third alternative is the correct one. Under conditions which limit proteolysis, 5-A["3H]T selectively ...

Energy Technology Data Exchange (ETDEWEB)

Scientists at JPL have developed chemical and biological techniques using microspheres filled with drugs, electron-opaque metals, or radioactive, fluorescent, magnetic or electrically charged materials to label specific groups of cells. Synthetic polymeric microspheres are coupled with specific antibodies to form reagents called immunomicrospheres, which can seek out and attach themselves to any specific group of cells. These cell-labeling techniques, therefore, open new avenues not only to the basic study of cells but also to the diagnosis and treatment of many diseases, including cancer.

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Photosynthetic carbohydrates from the leaves are exported through the phloem to growing tips, roots, flowers and fruits. If sucrose labelled with {sup 14}C is applied to the leaves of bean plants, the pathway for sugar movement may be readily observed by autoradiography. Students apply the labelled sucrose during class time and return the next day to press their plants. During the next class, the pressed and dried plants are placed against X-ray film and left in the dark for four weeks. the film is then developed, examined for presence of label and compared to the pressed plants. Source to sink movement is clearly illustrated and information about the mechanism of phloem transport and loading is gained through experimental treatments, which include blocking the phloem pathway and inhibiting energy production.

International Nuclear Information System (INIS)

Russian (1972). USSR Prokof'eva, MS (no.188) p. 217-222. age

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... ammonium compounds antigen-antibody reactions hemoglobin inhibition

International Nuclear Information System (INIS)

English 1999 p. 47 Brazil Oliveira, JF Braga, ACS Avila, ASR Bernardo-Filho,

UK PubMed Central (United Kingdom)

The binding of iodine-labelled plasminogen to Helicobacter pylori CCUG 17874 was characterized. Inhibition of the binding was observed after preincubation of H. pylori...Full Text Available

International Nuclear Information System (INIS)

1981. p. 21. France Ghanem, G. Roland, MT Libert, A. Gossuin, A. Maerschalk,

International Nuclear Information System (INIS)

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, "1"2"5I-azido-PS, and with an inhibitor of PS transport, "1"2"5I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

International Nuclear Information System (INIS)

Portuguese 1999 p. 18 Brazil Oliveira, JF Braga, ACS Bezerra, RJAC Bernardo

International Nuclear Information System (INIS)

Additional measurements have been made of deposition and retention of inhaled radioactively labeled iron oxide aerosols generated by a spinning top aerosol generator.

Science.gov (United States)

Advanced Solid Tumors With and Without Brain Metastases

International Nuclear Information System (INIS)

Purpose: To assess the prognostic value of biologic (p53, Ki-67) and clinical factors in squamous cell carcinoma of the oropharynx after radical surgery and postoperative radiotherapy (RT). Methods and Materials: Between 1985 and 1995, a total of 102 patients with 104 tumor sites were entered onto the study. Fifty-five primary tumors (53%) involved the tonsils, 26 (25%) the soft palate, and 23 (22%) the base of the tongue. Median age was 53 years (range 36-80 years). The clinical T- and N-categories (UICC 1997) were: T1 (30), T2 (47), T3 (22), T4 (5), N0 (33), N1 (28), N2 (42), and N3 (1). Histologically-clear margins were achieved in all patients by initial surgery. Postoperative RT to the primary and regional lymphatics was given, to a total of 60 Gy in 6 weeks, and single daily fractions of 2 Gy. The expression of the nuclear p53- and Ki-67-labeling index (LI) was investigated by immunostaining using the monoclonal antibodies DO-7 and MIB 1. ...

Science.gov (United States)

There are five tasks within this project on thermally stable coal-based jet fuels. Progress on each of the tasks is described. Task 1, Investigation of the quantitative degradation chemistry of fuels, has 5 subtasks which are described: Literature review on thermal stability of jet fuels; Pyrolytic and catalytic reactions of potential endothermic fuels: cis- and trans-decalin; Use of site specific {sup 13}C-labeling to examine the thermal stressing of 1-phenylhexane: A case study for the determination of reaction kinetics in complex fuel mixtures versus model compound studies; Estimation of critical temperatures of jet fuels; and Surface effects on deposit formation in a flow reactor system. Under Task 2, Investigation of incipient deposition, the subtask reported is Uncertainty analysis on growth and deposition of particles during heating of coal-derived aviation gas turbine fuels; under Task 3, Characterization of solid gums, sediments, and ...

International Nuclear Information System (INIS)

Synthesis of the azido substituted non-steroidal anti-inflammatory drug 2-(2,6-dichloroanilino)phenylacetic acid and isotope labeling of this compound have been performed and are described. Initial evaluation of the binding ability and photoreactivity indicates that this compound has potential for photoaffinity labeling as well as enzyme selectivity studies. (author).

International Nuclear Information System (INIS)

The authors have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with ["3H]tamoxifen aziridine (["3H]TAZ) were treated with trypsin (T), #alpha#-chymotrypsin (C), or Staphylococcus aureus V8 protease prior to electrophoresis on sodium dodecyl sulfate gels. Fluorography revealed a distinctive ladder of ER fragments containing TAZ for each protease generated from the M/sub r/ 66,000 ER. Immunoblot detection with the primate-specific antibody D75P3#gamma# revealed that all immunoreactive fragments corresponded to TAZ-labeled fragments but that some small TAZ-labeled fragments were no longer immunoreactive. In contrast, use of the antibody H222SP#gamma# revealed a correspondence ...

International Nuclear Information System (INIS)

This case report demonstrates an hepatic amebic abscess by scintigrapy, utilizing a new radiopharmaceutical designed specifically for that purpose. The abscess is delineated as a positive lesion after twenty four hours. The agent, "1"3"1I-labeled bromometronidazole, may prove to be specific for the diagnosis of these abscesses. (orig.).

Wastenet

...) Abstract: Fluorescence microscopic imaging is widely used in biomedical research to study molecular and cellular processes in cell culture or tissue samples. This is motivated by the high inherent sensitivity of fluorescence techniques, the spatial resolution that compares favorably with cellular dimensions, the stability of the fluorescent labels used and the sophisticated strategies that have been developed for selectively labeling target molecules. More recently, two and ...

UK PubMed Central (United Kingdom)

33 patients with advanced malignant melanoma were studied after intravenous administration of 131I-labeled Fab fragments specific for p97, an oncofetal glycoprotein of human melanoma. In all, 47 gamma...Full Text Available

Science.gov (United States)

A method is described to smear extremely thin layers of nuclear emulsion on labeled electron microscope preparations and to measure the thicknesses of these layers, Without further separation, preparation and emulsion can be observed after exposure and development in an electron microscope. The source of the tracks formed in the emulsion can be exactly identified and the size and structure determined. This method finds applications in dust research and also in the analysis of medical and biological sections. Further information can be obtained about properties of different emulsions. (auth)

UK PubMed Central (United Kingdom)

Radioimmunoassays for detecting cell-associated or released virus are described using either ¹²⁵I- or [³H]acetate-labeled antibodies. In the first assay system, antigen-antibody...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Research is described in the development of organometallic reagents in which the boron was attached to a nonreactive organic or inorganic matrix such as polystyrene, silica, or alumina. We developed the synthesis of oxygen-15 labelled butanol, which has been found to be a valuable blood flow agent in humans. We have also developed a series of polymeric borane derivatives which were used to prepare nitrogen-13 labelled amines.

International Nuclear Information System (INIS)

Two methods were used to label pig kidney microvillar membrane proteins from the luminal and cytoplasmic surfaces of closed membrane vesicles. The first method was lactoperoxidase-catalysed radioiodination. Lactoperoxidase and glucose oxidase were positioned inside or outside the vesicles, iodination being initiated by adding glucose and "1"2"5I. After electrophoresis of the proteins, asymmetric labelling patterns on radioautographs were observed. However the major disadvantage of this method was the high degree of intramembrane labelling of the fatty acid chains of membrane lipids. The second method overcame this disadvantage. A new hydophilic photoreagent, 3,5-di("1"2"5I)iodo-4-azidobenzenesulphonate, was transported by a Na"+-dependent system into microvillar vesicles, thus permitting labelling from either side of the membrane when the vesicles were photolysed. The activity of several microvillar ...

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The time of appearance of newly synthesized rRNAs and ribosomal proteins (r-proteins) in the ribosomes of pea cotyledons (Pisum sativum L.) during germination was investigated. The ribosomal fraction was isolated and analyzed according to the method of germination of the embryo in the presence of labeled precursors or after pulse labeling of the embryos at different stages of germination. For the identification of newly synthesized rRNAs in the ribosomes we estimated the relative stability of labeled RNAs to the action of RNase, the sedimentation rate, the ability to be methylated in vivo in the presence of (/sup 14/C)CH/sub 3/-methionine, and the localization in the subunits of dissociated ribosomes. The presence of newly synthesized r-proteins in the ribosomes was judged on the basis of the electrophoretic similarity in SDS-disc electrophoresis of labeled polypeptides of purified ribosome preparations ...

DEFF Research Database (Denmark)

Reactions of consumers to the appearance and taste of pork with and without information concerning outdoor production of pigs were tested in France, Denmark, Sweden and UK. Consumers in all four countries focussed on colour and fatness rather than marbling and drip to make their choice. Almost half of the British and Danish preferred the paler and the French the darker pork. Most people preferred the leaner pork. When information was provided in the form of labels, the vast majority of consumers preferred the pork labelled as originating from their own country as opposed to 'imported' and that labelled as pork from pigs 'raised outside' as opposed to 'inside'. There was no difference in the taste of grilled pork from indoor and outdoor production systems but pork labelled 'home produced' or 'outdoor' were more appreciated. Consumers' willingness to pay varied widely and was higher for those consumers ...

Energy Technology Data Exchange (ETDEWEB)

A new /sup 99m/Tc-phosphine-isocyanide complex with the general structure (/sup 99m/Tc (DEPE)/sub 2/(CNR)/sub 2/)/sup +/ has been synthesised and tested in animals and one human. In three animal species (rat, rabbit, dog), the complex is an efficient myocardial imaging agent, while in humans it remains in the blood pool. The complex is 100% protein bound in animals and humans, but whereas in humans it is attached to a 51.5 kdalton protein (probably prealbumin), in rabbits it appears to be bound to a larger macromolecule (M.W.>100 kdalton). The efficiency of the complex for blood pool labelling was tested in a human volunteer and compared with the standard in vivo red cell labelling technique with stannous pyrophosphate. A satisfactory radionuclide angiogram could be performed with less than 370 MBq of the complex. The count rate for the complex (cps/MBq) was 15% higher than that obtained with the labelled red cells and ...

International Nuclear Information System (INIS)

The authors discuss radioimmunoassays (RIAs) for eicosanoids widely used in the field of experimental, clinical and pharmacological research. The main drawback of RIAs is the possibility of low specificity. This was clearly demonstrated during the controversy on plasma levels of 6-keto-PGF_1 #alpha#, the stable hydrolysis product of PGI_2. When extensive purification including reverse phase high pressure liquid chromatography (rp-HPLC) was performed prior to the RIA for 6-keto-PGF_1 #alpha# the levels were at least one order of magnitude less than previously claimed, and did not support the so-called circulating hormone hypothesis.

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We investigate the radiative decays of the {phi}-meson to the scalar mesons a{sub 0}(980) and f{sub 0}(980). We demonstrate that, contrary to earlier claims, these decays should be of the same order of magnitude for a molecular state and for a compact state and, therefore, the available experimental information is consistent with both a molecular as well as a compact structure of the scalars. Thus, the radiative decays of the {phi}-meson into scalars establish a sizable K anti K component of the scalar mesons, but do not allow to discriminate between molecules and compact states. (orig.)

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It is pointed out that a recently claimed result regarding the removal of sharp structure in the electronic density of states N(E) of A-15 superconducting compounds at T approximately equal to Tsub(c) was in fact, shown earlier by one of the present authors (M.N.M.). Merely the variation of N(E) with energy in the vicinity of the Fermi level in these materials cannot explain their anomalous normal state properties and high values of lambda and Tsub(c). Rather a non-varying N(E) accompanied by phonon softening is in better agreement with experiment.

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A process is claimed for selectively preparing a mixture of two-carbon atom oxygenated hydrocarbons, namely, acetic acid, ethanol and acetaldehyde, by continuously contacting a gaseous reaction mixture containing hydrogen and carbon monoxide with a solid catalyst comprising rhodium in combination with one or more alkali metals selected from the group consisting of lithium, potassium, cesium and rubidium at reaction conditions correlated so as to favor the formation of a substantial proportion of such two-carbon atom products.

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We study the duality of N=1 gauge theories in the presence of a massless adjoint field. We calculate the superpotential using the factorization method and compare with the result obtained by applying Kutasov duality. The latter result is just the leading term of the former, indicating that Kutasov duality is exact only in the IR limit as claimed in the original literature. We also study various checks for the equivalence of the calculational methods developed recently: factorization methods, diagrammatic expansion, loop equations, integrating fluxes.

British Library Electronic Table of Contents (United Kingdom)

The quality of the end product from andrology services continues to lack consistency and in some cases fails to meet the needs of the end users (patients or clinicians). Results of external quality assessment (EQA) schemes continue to show unacceptably wide variation for the results of a single specimen. Some laboratories are able to show that the results of semen analyses relate to both natural and assisted pregnancy and are therefore useful in the management of the infertile couple, whereas others claim that their value is limited to the identification of severe male factor infertility. With wide variation in standardisation of methodology, levels of staff training and quality assurance, it is entirely understandable that such discrepancies persist. The following article proposes that Qu...

CERN Document Server

Information from the CHIS Board and Personnel Division:A new contract to manage our health insurance has been signed with AUSTRIA and it will come into force on 1 January 2000.The changeover to the new contract will not affect those covered by thescheme since there will be no change in the list of services reimbursed or in the reimbursement procedure.However, you are requested (as far as possible) to submit separate claims fortreatment (not bills) received before 1 January 2000 as opposed to treatment received after 1 January 2000. This will help AUSTRIA with its accounting.

British Library Electronic Table of Contents (United Kingdom)

The claim that focus evokes a set of alternatives is a central issue in several accounts of the effects of focus on interpretation. This article presents two empirical studies that examine whether this property of focus is independent of contextual conditions. The syntactic operation at issue is object-fronting in German, Spanish, Greek, and Hungarian licensed by contexts involving focus on the object constituent. This operation evokes the intuition that the fronted referent excludes some or all relevant alternatives. The presented experiments deal with the question whether this interpretative property obligatorily accompanies the operation at issue or not. The empirical findings show that in German, Spanish, and Greek this intuition depends on properties of the context and is sensitive to...

Energy Technology Data Exchange (ETDEWEB)

Bunker Hill Co. v. Environmental Protection Agency (EPA) challenged EPA's right to make on-site inspections. A review of the arguments in three Circuit Court Cases shows that the inspection would be meaningless if the chemical company retained exclusive control over inspections and the generated information, but neither court held the company in contempt for refusing admission to inspectors. Industry's claim that on-site monitoring is unconstitutional and the compromises in lawsuits suggest a lack of compliance by both industry and EPA in monitoring regulations. Congress needs to rewrite less-ambiguous regulations. 59 references. (DCK)

International Nuclear Information System (INIS)

Recently Basano (Int. J. Theor. Phys.; 16:715 (1977)) in a paper entitled 'Information Flow, Causality and the Classical Theory of Tachyons' commented on earlier work by the present authors. In answer to those comments it is pointed out that although 'Extended Relativity' seems to allow one to solve any causal paradoxes with both usual particles and tachyons nevertheless a number of paradoxes are continuously proposed. It has already been shown by the authors that tachyons possibly do not imply any causality violations even in macro-physics but Basano claimed that the procedure lead to new, different paradoxes. It is here demonstrated that such presumed difficulties do not exist. (U.K.).

British Library Electronic Table of Contents (United Kingdom)

The metaphysical dispute between moral realists and antirealists is cast in terms of properties: the realist holds that moral properties exist, the antirealist denies this claim. There is a longstanding philosophical dispute over the nature of properties, and the obscurity of properties may make the realist/antirealist dispute even more obscure. In the spirit of deflationary theories of truth, we can turn to a deflationary theory of properties in order to clarify this issue. One might reasonably worry that such an account of properties would not be capable of properly characterizing disputes regarding the existence or nonexistence of genuine moral properties. In this paper, I will show that, within this framework, the traditional disputes over the existence of moral properties can be chara...

International Nuclear Information System (INIS)

Insulitis is considered the histopathological hallmark of type I diabetes. In the non-obese diabetic (NOD) mouse, diabetes has never been observed in the absence of insulitis. The in vivo detection of insulitis could be of relevance for early prediction of diabetes. As approximately 15% of islet-infiltrating lymphocytes express interleukin 2 receptors, the authors have labelled recombinant inter-leukin 2 with "1"2"3I and used this radiopharmaceutical to detect insulitis by gamma camera imaging. The authors studied 71 prediabetic NOD and 27 normal Balb/c mice. Labelled #alpha#-lactalbumin was used as the control protein. In the first set of experiments the tissue distribution of radiolabelled interleukin 2 in isolated organs from animals sacrificed at different time points was studied. Higher radioactivity was detected in the pancreas of NOD mice injected with labelled interleukin 2, as compared to NOD mice receiving ...

International Nuclear Information System (INIS)

Four "1"2"5I labeled mono- and diamines were prepared and evaluated as potential brain-imaging agents. The diamines are analogues of the previously reported "7"5Se labeled diamines, which show high brain uptake and retention. All of the radioiodinated amines display high initial brain uptake in rats after intravenous injection (1.7-2.4% dose/organ). The xylylenediamines show prolonged brain retention (t1/2 approximately 18 h), which is desirable for brain imaging. In contrast, the benzylamine is rapidly cleared from brain tissue (t1/2 approximately 15 min).

International Nuclear Information System (INIS)

A brief survey is presented of the methods of preparation and of the applications of radiopharmaceuticals labelled with short-lived positron radionuclides "1"1C, "1"8F, "1"3N and "1"5O which, thanks to their energy level schemes, short half-life and the 511 keV photon radiation energy are almost ideal tracers in modern nuclear medicine and pharmacology. In conjunction with computerized tomography, they represent one of the most sensitive diagnostic imaging methods, the so-called positron emission tomography. In addition, their incorporation in the molecule of a pharmaceutical does not change the biological and chemical properties of the original molecule unlike those of radiopharmaceuticals labelled with technetium, iodine, etc. (author). 125 refs.

Energy Technology Data Exchange (ETDEWEB)

Wheat leaves can be induced by excision to produce fructans. Fructose residues of newly made oligofructans in leaves labeled in vivo with {sup 14}CO{sub 2} are not equally labeled. We report here on a fructosyl transferase activity in wheat leaves catalyzing the reaction: G{sup *}-F{sup *} + G-F-F = G{sup *}-F{sup *}-F + G-F. This activity, described previously in J. artichoke was attributed to fructan:fructan fructosyl transferase (FFT). The rate of this reaction in vitro is much higher than that of net kestose synthesis by SST. Hence, appearance of labeled 1-kestose from sucrose may not be an accurate measure of SST, but a curious reshuffling of hexoses between pools of 1-kestose and sucrose.

International Nuclear Information System (INIS)

Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymhocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of ["1"2"5I]iodonaphtylazide ["1"2"5I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymhpocytes with ["1"2"5I]INA and commercially available reagent 3-(trifluoromethyl)-3-(trifluoromethyl)-3-(3'-["1"2"5Iliodophenyl)diazirine (["1"2"5I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. (author). 28 refs.; 4 figs.

Energy Technology Data Exchange (ETDEWEB)

The iodinated oil lipiodol is commonly used as a carrier for in situ delivery of drugs or radioactivity to hepatic tumors. Recently, we reported a new kit formulation for high-activity labeling of lipiodol with the {beta}-emitting radionuclide Re-188. Since the whole preparation involves different steps and complex manipulations of high-activity samples, we describe here an automated synthesis module that allows the easy preparation of sterile and pyrogen-free samples of Re-188 lipiodol ready to be administered to the patient. Important advantages include the possibility to incorporate high Re-188 activity into the lipiodol hydrophobic phase and a sharp reduction of radiation exposure of the operator assisting the labelling procedure. Application of this modular reaction system could be also extended to the preparation of other Re-188 radiopharmaceuticals and to compound labelled with different {beta}-emitting therapeutic ...

International Nuclear Information System (INIS)

One approach in the design of neutral oxotechnetium complexes is based on the simultaneous substitution of a tridentate dianionic ligand and a monodentate monoanionic coligand on a [Tc(V)O]"+"3 precursor. Following this ''mixed ligand'' concept, a novel "9"9"mTc complex with N,N-bis(2-mercaptoethyl)-N'N'-diethylethylenediamine as ligand and 1-octanethiol as coligand is prepared and evaluated as potential brain radiopharmaceutical. Preparation of the complex at tracer level was accomplished by using "9"9"mTc-glucoheptonate as precursor. The substitution was optimized and a coligand/ligand ratio of 5 was selected. Under this conditions the labeling yield was over 80% and a major product (with radiochemical purity > 80%) was isolated by HPLC methods and used for biological evaluation. Chemical characterization at carrier level was developed using the corresponding rhenium complex as structural model. The Re complex was also prepared by ...

International Nuclear Information System (INIS)

Radioimmunotherapy (RIT) is a new treatment option for patients with non-Hodgkin lymphoma (NHL). Response to RIT currently remains difficult to predict using conventional prognostic factors and could be refined using functional imaging. The goal of this work is to evaluate the value of 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) in predicting response to Yttrium 90-labeled monoclonal antibodies for patients with NHL. Thirty-five patients with NHL who had undergone 18F-FDG PET prior to RIT with either 90Y-ibritumomab tiuxetan (group A; n=17) or 90Y-epratuzumab tetraxetan (group B; n=18) were included in this retrospective study. Four functional criteria were determined for each tumour lesion in a given patient: maximum and mean standard uptake values (SUVmax and SUVmean), functional lesion volume (LVol) and total lesion glycolysis (TLG, product of the volume and the SUVmean). For each patient, we determined highest SUVmax and SUVmean, cumulative ...

Energy Technology Data Exchange (ETDEWEB)

Radioiodinated 15-(p-iodophenyl)-3,3-dimethylpentadecanoic acid (DMIPP) has been prepared as a new terminal iodophenyl-substituted fatty acid containing dimethyl-branching at the beta-position. Iodine-125-labeled DMIPP showed rapid, high myocardial uptake (min, mean % injected dose/g) in fasted rats (5, 4.67; 30, 5.06; 60, 4.79; 120, 4.37), and also exhibited high heart:blood ratios (min, ratio) 5, 3:1; 30, 12:1; 60, 12:1; 120, 13:1. These data demonstrate an unanticipated much longer myocardial residence time with DMIPP (+/sub 1/2/ 7-8 h) than observed with either the 3-monomethyl (BMIPP) analogue (+/sub 1/2/ 30-45 min) or the rapidly metabolized straight-chain (IPP) analogue (+/sub 1/2/ 10-15 min). The (/sup 123/I)DMIPP is thus an excellent candidate for clinical evaluation of regional fatty acid metabolism under conditions where the uptake of energy substrates can be assessed independent of regional blood delivery. Studies with the new activated ...

International Nuclear Information System (INIS)

The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 10"9 to > 10"1"0 lmol) specifically directed against several DNA alkylation products with possible relevance in relation to both mutagenesis and malignant transformation of mammalian cells. These alkylation ...

Energy Technology Data Exchange (ETDEWEB)

This paper presents efforts to date and prospective goals towards development of a modelling and analysis framework which is comprehensive enough to address the global climate crisis, and detailed enough to provide policymakers with concrete targets and achievable outcomes. In terms of energy efficiency policy, this requires coverage of the entire world, with emphasis on countries and regions with large and/or rapidly growing energy-related emissions, and analysis at the 'technology' level-building end use, transport mode or industrial process. These elements have not been fully addressed by existing modelling efforts, which usually take either a top-down approach, or concentrate on a few fully industrialized countries where energy demand is well-understood. Inclusion of details such as appliance ownership rates, use patterns and efficiency levels throughout the world allows for a deeper understanding of the demand for energy today and, more importantly, over the ...

UK PubMed Central (United Kingdom)

A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to...Full Text Available

International Nuclear Information System (INIS)

Using nitrogen-13 initial measurements have been made of rumen ammonia-nitrogen pool size and turnover, and rate of incorporation of ammonia into microbial protein. It is also feasible to measure the rate of incorporation of absorbed ammonia into urea and the appearance of label in saliva.

UK PubMed Central (United Kingdom)

The metabolism of ¹⁴C-labeled fatty acids and triacylglycerols was followed in intact germinating oil palm seedlings as well as in tissue slices. In the germinating seedling, the shoot contained...Full Text Available

International Nuclear Information System (INIS)

... Technology; Warsaw (Poland) 978-83-909690-8-4 221 p. RADIOLOGY AND

UK PubMed Central (United Kingdom)

By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [³H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have...Full Text Available

UK PubMed Central (United Kingdom)

The adenovirus fiber knob causes the first step in the interaction of adenovirus with cell membrane receptors. To obtain information on the receptor binding site(s), the interaction of labeled cell...Full Text Available

International Nuclear Information System (INIS)

Platelet deposition on bovine pericardial-tissue mitral-valve prostheses in 11 dogs was observed noninvasively by use of "1"1"1In-labeled platelets and quantified after sacrifice at one (n . 3), 14 (n . 3), and 30 (n . 5) days postimplantation (300-400 microCi of labeled platelets having been injected 24 hours previously). Thrombosis on the sewing ring and pericardial leaflets at one and 14 days and on the leaflets at 30 days was delineated in scintiphotos. In vitro quantification (% injected dose) indicated that the leaflets, sewing ring, and perivalvular tissue retained 0.904% of labeled platelets at one day postimplantation, 0.198% at 14 days, and 0.040% at 30 days. Platelet half-life was reduced to 38 hours at 21 days postimplantation but returned toward the normal (50 hours) with fibrous ingrowth in the sewing ring. Microembolism in lung and kidney, as measured by tissue/blood radioactivity ratio, also was decreased ...

UK PubMed Central (United Kingdom)

Skin sensitization is the most commonly reported occupational illness, causing much suffering to a wide range of people. Identification and labeling of environmental allergens is urgently required to...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundZibotentan (ZD4054) is a specific endothelin A (ET_A) receptor antagonist being investigated for the treatment of prostate cancer. As zibotentan is eliminated...Full Text Available

UK PubMed Central (United Kingdom)

To gain insight into the process of mitochondrial transmission in yeast, we directly labeled mitochondrial proteins and mitochondrial DNA (mtDNA) and observed their fate after the fusion of two cells....Full Text Available

UK PubMed Central (United Kingdom)

The somatic sensory system responds to stimuli of distinct modalities, including touch, pain, itch, and temperature sensitivity. In the past century, great progress has been made in understanding the...Full Text Available

UK PubMed Central (United Kingdom)

To investigate the fate of intrinsic factor and cobalamin during cobalamin absorption, we incubated enterocytes isolated from guinea pig ileum for periods of up to 30 min with ⁵⁷Co-labeled...Full Text Available

International Nuclear Information System (INIS)

Portuguese 2006 1 p. Brazil Rebello, BM Florentino, DM Godinho, CR

Energy Technology Data Exchange (ETDEWEB)

The sucrose derivative 6'-deoxy-6'-(2-hydroxy-4-azido)benzamidosucrose (6'-HABS) was prepared from sucrose (via 6'-deoxy-6'-aminosucrose) and 4-amino-salicylic acid. 6'-HABS is a competitive inhibitor of sucrose influx into protoplasts from developing soybean cotyledons and of sucrose binding to membranes from the bacteria P. saccharophila. The Ki for inhibition in the soybean protoplasts was 75..mu..M. 6'-Deoxy-6'-(2-hydroxy-3-/sup 125/Iodo-4-azido)benzamidosucrose was prepared by lactoperoxidase iodination of 6'-HABS. Upon photolysis in the presence of membranes from P saccharophila, label from the photoprobe is incorporated into a sucrose inducible polypeptide of mass 84 KD in SDS-PAGE. The polypeptide is protected from labeling by the inclusion of sucrose in the photolysis mixture. Photolysis conditions which lead to specific labeling of ...

International Nuclear Information System (INIS)

This paper describes the evaluation of antibody coated polystyrene beads using "1"2"5I-labelled T_3 ("1"2"5I-T_3). (author).

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

UK PubMed Central (United Kingdom)

The Calorie (kcal) of present U.S. food labels is similar to the original French definition of 1825. The original published source (now available on the internet) defined the Calorie as the quantity...Full Text Available

International Nuclear Information System (INIS)

Dairy products doubly labeled with 45Ca and 47Ca were used to evaluate an extrinsic labeling procedure for calcium bioavailability determination. Nonfat milk, yogurt, and fresh cheese curd were prepared from caprine milk that was intrinsically labeled with 45Ca. The products were then labeled extrinsically with 47Ca and administered to rats by gavage. The 47Ca to 45Ca ratio in bone and teeth averaged about 1.00 with either milk, yogurt, or CaCl2, but the ratio was about 1.04 when dosed with cheese curd. Ca absorption, determined by whole-body counting of 47Ca, was lower (P less than 0.05) in cheese curd (59%) than in either milk (69%), yogurt (72%), or CaCl2 (72%). Expressed as percent of dose, the absorption of 47Ca was highly correlated with bone 47Ca (r = 0.973) and with bone 45Ca (r = 0.946). Correlation between tibia 47Ca and tibia 45Ca was r = 0.923. For the dairy products tested, our results ...

UK PubMed Central (United Kingdom)

A solid-phase radioimmunoassay employing ¹²⁵I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies...Full Text Available

UK PubMed Central (United Kingdom)

This paper reviews the development of cerebral scintigraphy from its early days of planar imaging with simple technetium-99m labelled compounds to the recent revival of the technique in the form of...Full Text Available

UK PubMed Central (United Kingdom)

¹⁴C has long been used as a tracer for quantifying the in...Full Text Available

UK PubMed Central (United Kingdom)

Amino acids labelled with dimethylaminoazobenzenesulphonyl chloride can be separated by reversed-phase high-pressure liquid chromatography and detected in the visible region (436 nm). All 19 naturally...Full Text Available

International Nuclear Information System (INIS)

Six kinds of diamino-dithiol compounds are labelled with "9"9Tc"m by ligands exchange reaction. Their ligands exchange percentage is over 95%. The biodistribution of one of these compounds in mice shows 0.88% brain uptake after two minutes and 0.28% after 15 minutes respectively. Although the brain uptake is lower for this compound, it has longer retention in brain. So, the experiment results are valuable to the design of new brain imaging agent in the future.

International Nuclear Information System (INIS)

We describe a compact ultrafiltration system for the removal of pyrogens and bacteria from water labeled with the stable isotopes of deuterium and oxygen-18. The ultrafiltration system is constructed from readily available commercial components and can achieve complete removal of pyrogens and bacteria from 1L contaminated water within 30 min. By use of our procedure, loss of the isotopically labeled water by retention in the filtration system was minimal. The purified water is suitable for both oral and intravenous administration to healthy human subjects participating in nutrition studies.

International Nuclear Information System (INIS)

An azido-"1"2"5iodo-ansamycin (1) in the geldanamycin family was prepared in two steps in one reaction vessel from 17-amino-22-(4'-aminophenacyl)-17-demethoxygeldanamycin (3). The title compound was suitable for photoaffinity labeling proteins that interacted with ansamycins. An alternative synthesis is reported for preparation of unlabeled title compound in order to afford practical amounts of (1) for conventional biochemical studies. (Author).

Energy Technology Data Exchange (ETDEWEB)

Our major goal of our work has been to develop and use NMR techniques to study how methanogenic archaebacteria deal with osmotic stress with the hope of providing insights into increasing the salt tolerance of other cells. The project has three main sections: (i) in vivo studies of methanogens; (ii) use of {sup l3}C- and {sup l5}N- labeled potential precursors and in vitro analyses of specific label uptake for elucidation of osmolyte dynamics and biosynthetic pathways of osmolytes in these organisms, and isolation of key biosynthetic enzymes; and (iii) collaborative studies on identification of organic solutes in other methanogens.

Energy Technology Data Exchange (ETDEWEB)

Possibilities of applying the Pb/sup 203/ isotonic solution without a carrier as a label of blood erythrocytes, are found. There is a number of advantages in the Pb/sup 203/ isotonic solution without a carrier, such as a high label efficiency (above 95%), a short half-life (52 hours), and a low radiation dose. These factors prove to be favourable as compared with Cr/sup 51/ widely used for these purposes. Besides, of all preparations used up to now to treat erythrocytes and to scan the spleen, it is only in the case of Pb/sup 203/ isotonic solution without a carrier that no weight amounts of chemical substances do not enter the organism.

International Nuclear Information System (INIS)

In the use of doxorubicin and radiation for treatment of human malignant tumors in vivo, the relationship between treatment-induced apoptosis and Ki-67 labeling index was investigated. Four human tumor xenografts (ependymoblastoma, NNE; primitive neuroectodermal tumor, YKP; small cell lung carcinoma, GLS; glioblastoma, KYG) were transplanted under the skin of thigh of the nude mice (BALB/cA JcL-nu). The mice were given a single radiation dose of 1 Gy, or doxorubicin alone intraperitoneally at a dose of 8 mg/kg. After treatment, sections of tumor specimens were prepared from paraffin-embedded tissues. Hematoxylin and eosin staining, TUNEL staining, and immunohistochemical analysis of Ki-67 were performed. In NNE, apoptotic cells appeared most frequently after treatment compared with all other tumors, and the incidence of apoptosis in the radiation-treated group was much higher than in the doxorubicintreated group. As the incidence of apoptosis in NNE increased, the ...

International Nuclear Information System (INIS)

The purpose of this work was to develop a high specific activity radioiodinated cell membrane probe for tracking lymphocytes in-vivo to replace the nucleus localizing, cytotoxic lipophilic chelates (In-111 oxine and Tc-99m HMPAO) currently used. Alkylation of parent dye 4-[2-[-N,N-didecylamino]phenyl]ethenyl pyridine with E-1-tributylstannyl-3-tosylpropene (prepared form E-1-tributylstannyl-1-propene-3-ol), gave a tributyltin precursor 1. Radiolabeled 3-[4-[2-[4-(N,N-didecylamino)phenyl]ethenyl]pyridino] E-[I-125]-1-iodopropene (2), was prepared from 1 using peracetic acid in acetonitrile/water. Labeling yields and specific activities achieved were 26% (#approx#2170 Ci/mmol), 40% (1220 Ci/mmol), and 55% (200 Ci/mmol) for nca, 0.4, and 2 nanomole carrier iodide runs respectively. Canine mixed leukocytes (0.5-1.0 x 10"8 cells) were labeled with 2 (67% and 42% yields for 200 Ci/mol and 1220 Ci/mmol preparations) and showed blood clearance similar ...

International Nuclear Information System (INIS)

The use of natural products in all over the world has been increased in Brazil as well as in other countries. Maytenus ilicifolia is commonly used in popular medicine. The labeling of red blood cells (RBC) with technetium-99m ("9"9"m Tc) have been for many studies in nuclear medicine. This labeling procedure depends on a reducing agent and stannous chloride is normally used. Here, we investigate if the extract of Maytenus ilicifolia is capable to alter the labeling of RBC and blood proteins with "9"9"m Tc. Blood samples were incubated with Maytenus ilicifolia. Stannous chloride solution and Tc-99m were. Blood was centrifuged and plasma (P) and blood cells (C) were isolated. Samples of P or C were precipitated with trichloroacetic acid, centrifuged and IF and IF were separated. The percentage of radioactivity (% ATI) in C, IF-P and IF-C was calculated. The %ATI in decreased in C from 93.6#+-#2.3 to 29.0#+-#2.7, on FI-P from ...

Energy Technology Data Exchange (ETDEWEB)

It is an object of the claimed invention to combine chemical treatment with microfiltration process to treat groundwater, leachate from contaminated soil washing, surface and run-off waters contaminated with toxic metals, radionuclides and trace amounts of organics from variety of sources. The process can also be used to treat effluents from industrial processes such as discharges associated with smelting, mining and refining operations. Influent contaminants amenable to treatment are from a few mg/L to hundreds of mg/L. By selecting appropriate precipitation, ion exchange and adsorption agents and conditions, efficiencies greater than 99.9 percent can be achieved for removal of contaminants. The filtered water for discharge can be targeted with either an order of magnitude greater or lower than contaminant levels for drinking water.

Science.gov (United States)

Studies on the effects of electromagnetic fields (EMF)on human health have been conducted for more than 20 years. None have categorically established or eliminated any connection between EMF and adverse health effects. Nonetheless, 96% of the risk managers working in the utility sector view EMF as an over-riding concern. Public concern over EMF has erupted into legal, as well as regulatory, actions. To date, some utilities have had lawsuits brought against them by customers who claim they developed cancer as a result of nearby high-voltage transmission lines. What`s more, a small handfull of cities have adopted ordinances that specifically limit where and how utilities handle the placement of their transmission lines.

British Library Electronic Table of Contents (United Kingdom)

The issue of the duration of immunity, particularly for the modified live viral components of veterinary vaccines, has been a significant part of the recent vaccination debate. One manufacturer has increased the recommended booster interval for these components to 3 years give name and another now states `up to 4 years' immunity.There remain many unanswered questions regarding this duration of immunity (DOI). Studies suitable for data sheet claims are time consuming and costly and can only be performed in laboratory dogs under tightly controlled conditions. Evidence from rabies serology testing in the UK shows that the response of individual animals to routine vaccination is highly variable. Much of the published field evidence on the persistence of antibody titres originates from North Am...

Energy Technology Data Exchange (ETDEWEB)

This process for the preparation of nonaqueous dispersions of water-soluble polymerisates concerns compounds obtained by polymerization of water-soluble unsaturated ethylenic monomers in an emulsion of water in oil. The product is then dewatered to a maximum residual water content of 5% by means of microwaves. The process is azeotropic. The polymer has a molecular weight between 100,000 and 50,000,000, preferably between one and 10 million. Before treating with microwaves, the mixture is concentrated by conventional means until it has a polymer concentration between 20 and 65%, preferably between 40 and 60%. The microwaves have a wavelength between 0.1 and 30 cm. These polymer dispersions can be used in drilling fluids and as additives in tertiary oil recovery. 4 claims.

British Library Electronic Table of Contents (United Kingdom)

This article reviews the little examined early work of the Greek Marxist and state theorist, Nicos Poulantzas (1936-1979). In his first book, Nature du choses et droit of 1965, the young scholar developed a sociology of law culled from the insights of philosophical ontology. The article sets out the central claims of that book and reflects on its place in Poulantzass intellectual development. Drawing on Heidegger, Sartre and Marx, Poulantzas proposed a species of Natural Law theory that unified `facts' and `values' by grounding legal concepts in a theory of social praxis centred on material labour. Legal categories were thus irreducible to ahistorical essences but were, rather, expressions of mankinds struggle to realize its intrinsic freedom. As we shall see, although flawed and in key re...

CERN Document Server

Community ecologists are principally occupied with the proposition that natural assemblages of species exhibit orderliness and with identifying its causes. Plant-pollinator networks exhibit a variety of orderly properties, one of which is 'nestedness'. Nestedness has been attributed to various causes, but we propose a further influence arising from the phylogenetic structure of the biochemical constraints on the pollen diets of bees. We use an artificial assemblage as an opportunity to isolate the action of this mechanism. The properties of the network that we studied are consistent with the proposition that nestedness is caused by the phylogeny of diet range in bees, but the claim is preliminary and we propose that valuable progress in understanding plant-pollinator systems may be made through applying the techniques of chemical ecology at the community scale.

British Library Electronic Table of Contents (United Kingdom)

This paper opens with a critical analysis of a paradox in contemporary educational research in and about Confucian Heritage Culture (CHC): the assumption that national boundaries coincide with those of a distinct and homogeneous culture, which consistently renders a rather homogenous set of educational phenomena, and collides against a more widely accepted discourse - culture transcends geographical frontiers and is ever evolving in character. It is claimed that this paradox is due to the fact that a thin conception of CHC competes neck-and-neck with a thick conception of it. This paper also addresses the possibility of an ad hoc education research methodology in and about CHC and its compliance issues regarding the mainstream Western research dynamics and philosophy of science. Confucian ...

CERN Document Server

Bargmann's superselection rule, which forbids the existence of superpositions of states with different mass and, therefore, implies the impossibility of describing unstable particles in non-relativistic quantum mechanics, arises as a consequence of demanding Galilean covariance of Schr\\"odinger's equation. However, the usual Galilean transformations inadequately describe the symmetries of non-relativistic quantum mechanics since they fail to take into account relativistic time contraction effects which can produce non-relativistic phases in the wavefunction. In this paper we describe the incompatibility between Bargmann's rule and Lorentz transformations in the low-velocities limit, we analyze its classical origin and we show that the Extended Galilei group characterizes better the symmetries of the theory. Furthermore, we claim that a proper description of non-relativistic quantum mechanics requires a modification of the notion of spacetime in the corresponding ...

International Nuclear Information System (INIS)

A large detector with high redundancy is used to search for various types of anomalous particles in cosmic rays at sea level. The detector is sensitive to zenith angles between 45"0 and 90"0. Previously obtained limits on the fluxes of charge (1/3) and (2/3) particles are reduced to 2.9 x 10"-"1"0 and 2.6 x 10"-"1"0 cm"-"2sr "-"1 sec"-"1, respectively. The flux of ionizing tachyons is determined to be less than 2.4 x 10"-"9 cm"-"2 sr"-"1 sec"-"1. The massive-particle flux limit we obtain is inconsistent with previous claims of such particles assuming that these particles are isotropic in zenith angle.

British Library Electronic Table of Contents (United Kingdom)

Abstract- In our reply to M. H. van IJzendoorn and M. J. Bakermans-Kranenburg (this issue) and R. A. Thompson (this issue), we highlight 2 challenges that attachment researchers face today: (a) closing the gap between the developmental and social psychological traditions and (b) connecting attachment theory to the broader field of evolutionary psychology. We contend that an evolutionary life history approach can contribute to both goals and argue that attachment researchers should consider moving beyond some of Bowlby's original formulations in order to permit further advancement of the field. Finally, we review van IJzendoorn and Bakermans-Kranenburg's criticism of the hypothesis that sex differences in attachment arise in middle childhood; we conclude that the claim that the hypothesis h...

CERN Document Server

We investigate the effect of the intrinsic spin of a fundamental spinor field on the surrounding spacetime geometry. We show that despite the lack of a rotating stress-energy source (and despite claims to the contrary) the intrinsic spin of a spin-half fermion gives rise to a frame-dragging effect analogous to that of orbital angular momentum, even in Einstein-Hilbert gravity where torsion is constrained to be zero. This resolves a paradox regarding the counter-force needed to restore Newton's third law in the well known spin-orbit interaction. In addition, the frame-dragging effect gives rise to a {\\it long-range} gravitationally mediated spin-spin dipole interaction coupling the {\\it internal} spins of two sources. We argue that despite the weakness of the interaction, the spin-spin interaction will dominate over the ordinary inverse square Newtonian interaction in any process of sufficiently high-energy for quantum field theoretical effects to be ...

Science.gov (United States)

Fires in the operating room continue to present a hazard to patients, at times with catastrophic and debilitating results. Recent data from closed claim files reveal oxygen, electrosurgical unit (ESU), and surgical drapes are common components of the fire triangle in the operating room. In this era of biotechnological sophistication, why are surgical drapes flammable? The purpose of this study was to test the flammability of different surgical drape materials and to determine the time to ignition using a bipolar ESU device in 21%, 35%, and 100% oxygen concentrations. Results show that regardless of oxygen concentration surgical drapes, when exposed to close contact with the ESU, are flammable. Time to ignition decreases with increasing concentrations of oxygen as expected. One of the surgical drapes tested was advertised to the hospital as nonflammable. Future research should focus on surgical drape materials and aim to reduce the flammability of such items in the ...

Science.gov (United States)

These three separate pamphlets provide background information, brief discussions of research findings, and guidelines and recommendations concerning selected aspects of diet. The first pamphlet discusses food additives and hyperactivity, focusing on both the Feingold theory and controlled experiments which do not support Feingold's clinical observations and case reports. (The Feingold theory claims that artificial food flavors and colors and antioxidant preservatives cause hyperactivity in genetically predisposed individuals.) The second pamphlet discusses fast food and the American diet as they are related to sound nutritional principles. The third pamphlet, focusing on alcohol use during pregnancy, lists principles and associated features of the fetal alcohol syndrome and presents research findings and recommendations concerning alcohol use during pregnancy. (RH)

International Nuclear Information System (INIS)

Aim: Compounds such "9"9"mTc-MDP and "9"9"mTc-HEDP are used regularly in bone scintigraphy for metastasis detection. The therapeutics properties of beta emitter radionuclides like "1"5"3Sm, "1"6"6Ho and "1"7"7Lu has been widely reported in literature being "1"7"7Lu the less developed for medical applications. With the purpose of study different radiopharmaceuticals alternatives, for metastasis bone palliation, we have evaluated, on a comparative basis, the labeling of "1"5"3Sm-MDP, "1"5"3Sm-HEDP, "1"6"6Ho-MDP, "1"6"6Ho-HEDP, "1"7"7Lu-MDP and "1"7"7Lu-HEDP from the exhibited radionuclidic purity and biological distribution point of view. Material and Methods: The radionuclides were produced at La Reina Research Reactor, Chilean Nuclear Energy Commission. The radionuclidic purity was determined by gamma-Ray spectrometry. The labeling was accomplished with MDP (Plenum) and HEDP synthesized in-house. The in-vitro affinity of ...

Energy Technology Data Exchange (ETDEWEB)

The murine 3T3-L1 fibroblast under appropriate incubation conditions differentiates into an adipocyte phenotype. This 3T3-L1 adipocyte exhibits many of the morphologic, biochemical, and insulin-responsive features of the normal rodent adipocyte. Using quantitative electron microscopic (EM) autoradiography we find that, when /sup 125/I-insulin is incubated with 3T3-L1 adipocytes, the ligand at early times of incubation localizes to the plasma membrane of the cell preferentially to microvilli and coated pits. When the incubation is continued at 37 degrees C, /sup 125/I-insulin is internalized by the cells and preferential binding to the villous surface is lost. With the internalization of the ligand, two intracellular structures become labeled, as determined by the method of hypothetical grain analysis. These include large clear, presumably endocytotic, vesicles and multivesicular bodies. Over the first hour of incubation the labeling of these ...

International Nuclear Information System (INIS)

An improved labeling procedure for peptides attached to organometallic 99mTc(III) '4+1' mixed-ligand complexes in which the radiometal is coordinated by a tripodal tetradentate chelator 2,2',2''-nitrilotriethanethiol (NS3) and a monodentate isocyanide ligand is presented. The labeling procedure was evaluated by the synthesis of [99mTc(NS3)(L2-RGD)]. The containing radiopharmaceutically interesting RGD-peptide cyclo[Arg-Gly-Asp-D-Tyr-Lys] was modified with 4-isocyanobutanoic acid (L2) as linker conjugated to N6-Lys to get the monodentate ligand L2-RGD. The structural identity of the 99mTc-conjugate was confirmed by comparison to a Re reference compound. The Tc- and Re-conjugates had matching retention times under identical HPLC conditions. The 99mTc-labeling was performed in a novel one-step procedure using the eluate of a 99Mo/99mTc generator, NS3, the isocyanide modified peptide, SnCl2, Na2EDTA, mannitol and ascorbic acid ...

International Nuclear Information System (INIS)

A carrier-free radioiodinated phenylazide derivative of forskolin, 3-iodo-4-azidophenethylamido-7-O-succinyl-deacetyl-forskolin (["1"2"5I]IAPS-forskolin), has been shown to be a highly selective photoaffinity probe for the human erythrocyte glucose transported and the glucose transport proteins found in several mammalian tissues and cultured cells where the glucose transport protein is present at a low concentration. The photoincorporation of ["1"2"5I]IAPS-forskolin into these glucose transporters was blocked by D- (but not L-) glucose, cytochalasin B, and forskolin. In addition to labeling the mammalian glucose transport proteins, ["1"2"5I]IAPS-forskolin also labeled the L-arabinose transporter from E. coli. In muscle and adipose tissues, glucose transport is markedly increased in response to insulin. ["1"2"5I]IAPS-forskolin was shown to selectivity tag the glucose transporter in membranes derived from these cells. In addition, the covalent ...

International Nuclear Information System (INIS)

The measurement of physiological parameters in man is possible with the help of positron emission tomography (PET) and radiopharmaceuticals labeled with short lived positron emitters as C 11, N 13, O 15 and F 18. With the use of this substances it is possible to make a tomographic map defining regional metabolic parameters in normal and diseased brain. This technique has therefore also be named 'in vivo autoradiography'. The possibility of applying C 11 or F 18 labeled deoxyglucose with PET for detecting regional and local changes in cerebral metabolic rate of glucose in brain development in children of 5 days to 1 year of age is discussed. Beyond this a relationship between cerebral metabolic rate of glucose, cerebral blood flow and cerebral metabolic rate of oxygen by use of this technique after inhalation of O 15 and C 11-labeled CO_2 is shown. Attention is drawn to the application of C 11-methyl-spiperone and PET to ...

Energy Technology Data Exchange (ETDEWEB)

Sucrose and mannitol are major photosynthetic products and translocates in celery. Assimilate partitioning and transport were studied by pulse-labeling leaves with /sup 14/CO/sub 2/ followed by different length chases in ambient air. After a 2 h chase in the light there was more /sup 14/C in sucrose than mannitol in source leaves and their petioles. In contrast after a 2 h dark chase leaves contained more /sup 14/C in mannitol than sucrose but petioles had more /sup 14/C in sucrose than mannitol. After a 15 h chase (6 h light; 9 h dark) labeled sucrose was higher in source petiole vascular bundles than in adjacent parenchyma tissue but label in glucose and fructose was higher in the parenchyma tissue. After the 15 h chase most of the /sup 14/C remaining in developing sink leaves and their petioles was in mannitol. Although in the light mannitol:sucrose ratios are the same in leaf and petiole tissues, in the dark sucrose is ...

International Nuclear Information System (INIS)

Gel filtration of human erythrocyte (RBC) lysate incubated with labeled thyroxine (Tu) or triiodothyronine (Tt) revealed co-elution of a major iodothyronine-binding fraction (R-2) and hemoglobin. Solutions of purified human hemoglobin and Tt also showed co-elution of hormone and hemoglobin. Because hematin and protoporphyrin were shown to bind labeled Tt, the oxygen-binding site on hemoglobin was excluded as the site of iodothyronine-hemoglobin interaction. Analysis of hormone binding by heme and globin moieties showed Tt binding to be limited to the heme fraction. Addition of excess unlabeled Tt to hemoglobin or heme incubated with labeled Tt indicated 75% to 90% of hormone binding was poorly dissociable. These observations suggested that the presence of hemoglobin in RBC lysate or in serum could influence the measurement of Tu and Tt by specific radioimmunoassay (RIA). Subsequent studies of the addition to serum of human ...

Energy Technology Data Exchange (ETDEWEB)

The effects of mouse epidermal growth factor (EGF) on bone formation and resorption were examined in male mice. EGF administration (2-200 ng.g-1.day-1 ip for 7 days) induced a dose-dependent rise in plasma EGF levels that remained within physiological range. Histomorphometric analysis of caudal vertebrae showed that EGF (20 and 200 ng.g-1.day-1) reduced the endosteal matrix and mineral appositional rates after 5 days of treatment as measured by double (3H)proline labeling and double tetracycline labeling, respectively. This effect was transitory and was not observed after 7 days of EGF administration. EGF administered for 7 days induced a dose-dependent increase in the periosteal osteoblastic and tetracycline double-labeled surfaces. At high dosage (200 ng.g-1.day-1) EGF administration increased the osteoclastic surface and the number of acid phosphatase-stained osteoclasts, although plasma calcium remained normal. The ...

Energy Technology Data Exchange (ETDEWEB)

Dynamics and metabolism of sugars translocated into the fruit region were investigated in tomato using {sup 14}C-labelled compounds. The translocation substance in tomato is thought to be sucrose, but the amount of sucrose in its fruit is very small. Therefore, the degradation of sucrose in the fruit and its translocation to the fruit was investigated. The sucrose was digested into hexoses such as glucose, fructose, etc. and the degradation rate was considerably low. In the stage of thickening growth, sucrose was more extensively converted into other substances except for sugars compared with the coloring stage. Then, {sup 14}C-labelled fructose was given through its gynophore to investigate the degree of reproduction to sucrose. The fructose level in the fruit reached the platean by 48 hrs after the addition and gradually decreased. The radioactivities were detected in sugars, organic acids and other alcohol soluble substances, etc. These ...

Energy Technology Data Exchange (ETDEWEB)

The circulation in microvascularized rib grafts has been compared with that in conventional rib grafts and in those augmented by a direct vascular bundle implantation into the bone grafts. A new experimental model has been designed to correlate vascular perfusion, bone scan patterns, tetracycline labeling, and histological findings in these bone grafts. Posterior microvascularized rib grafts were found to have a circulatory pattern identical to that of the normal rib. Failed microvascularized rib grafts were revascularized more slowly than conventional rib grafts. Vascular bundles implanted into rib grafts remained patent and increased the rate of revascularization. The stripping or preservation of periosteum had no observable effects on the rate or pattern of conventional rib graft revascularization. The circulation in rib grafts was accurately reflected in technetium 99 bone scans, as was the patency of the anastomoses of microvascularized rib grafts and of ...

CERN Document Server

If a closed smooth manifold $M$ with an action of a torus $T$ satisfies certain conditions, then a labeled graph $\\mG_M$ with labeling in $H^2(BT)$ is associated with $M$, which encodes a lot of geometrical information on $M$. For instance, the "cohomology" ring $\\mHT^*(\\mG_M)$ of $\\mG_M$ is defined to be a subring of $\\bigoplus_{v\\in V(\\mG_M)}H^*(BT)$, where $V(\\mG_M)$ is the set of vertices of $\\mG_M$, and is known to be often isomorphic to the equivariant cohomology $H^*_T(M)$ of $M$. In this paper, we determine the ring structure of $\\mHT^*(\\mG_M)$ when $M$ is a flag manifold of classical type directly without using the fact that $\\mHT^*(\\mG_M)$ is isomorphic to $H^*_T(M)$.

International Nuclear Information System (INIS)

Two peptide ligands conjugated adenine, [9-N-(tritylmercapto acetyl diglycyl aminoethyl) adenine, Tr-MAG_2-Ade] and [9-N-(tritylmercapto acetyl triglycyl aminoethyl) adenine, Tr-MAG3-Ade], are synthesized and labeled with "9"9"mTc by directly labeling method. The stability of "9"9"mTc-MAG_2- adenine and "9"9"mTc-MAG_3-adenine in vitro is measured. The uptake radios of tumor to muscle at 3h post-injection are 5.70 and 4.92, respectively. The biodistribution and scintigraphic imaging studies show that the two complexes have high localization in tumor and high contrasted tumor images can be obtained, which suggest their potential utility as tumor imaging agents. But the high radioactivity of abdomen could prevent the tumor imaging in this area. (author)

International Nuclear Information System (INIS)

Eight patients with double-velour Dacron aorto-femoral grafts implanted for 2-60 months were studied. Indium-111-labelled platelets were found to have a shortened mean platelet lifespan (MPLS) of 138 +- 32 hours compared with the normal platelet lifespan of 224 +- 23 hours. Labelled platelets accumulated on all grafts, irrespective of graft age, to a maximum of 1,80 +- 0,83% of whole body radioactivity; thereafter graft radio-activity decreased. This suggests that platelets on the graft are in dynamic equilibrium with those in the circulation. Platelets may be lost from the graft by embolization or radioactivity may be released because of platelet lysis. Although MPLS may depend on the age and surface area of the graft, concomitant arterial disease probably also contributes to the shortened MPLS.

International Nuclear Information System (INIS)

Mineralization and volatilization of ring labelled "1"4C-2,4-dichlorophenoxyacetic acid in soil was studied over a period of six weeks under laboratory conditions at 25 deg. C in three different soils collected from three sites, Delhi, Jaipur and Ludhiana. A very slow rate of both mineralization and volatilization was observed in all the three soils. The observed mineralization, was highest for the Delhi soil, 0.93%, followed by the Ludhiana soil, 0.73% and the Jaipur soil 0.14% in 42 days. The extent of volatilization was 0.46% for the Jaipur soil, 0.37% for the Ludhiana soil and 0.32% for the Delhi soil. (author)

International Nuclear Information System (INIS)

Labelling of "1"4C-M-ISP and its absorption, translocation and distribution in peanut and soybean seedling were studied. The results of experiments showed that the radioactive substance could be translocated in xylem and phloem of two kinds of plants. The root of peanut seedling absorbed "1"4C-M-ISP from the nutrient solution more strong than the root of soybean seedling did. After leaf treatment with "1"4C-M-ISP, the soybean seedling absorbed more radioactive substance than peanut seedling did. But the translocation's speed of radioactive substance in pnloem of two kinds of plants were similar.

Science.gov (United States)

Hydrogen cyanide (HCN) production by Pseudomonas aeruginosa in a synthetic medium is stimulated by the presence of glycine. Methionine enhances this stimulation but will not substitute for glycine as a stimulator of cyanogenesis. Threonine and phenylalanine are effective substitutes for glycine in the stimulation of HCN production. Glycine, threonine, and serine are good radioisotope precursors of HCN, but methionine and phenylalanine are not. Cell extracts of P. aeruginosa convert (/sup 14/C)threonine to (/sup 14/C)glycine. H14CN is produced with low dilution of label from either (1-/sup 14/C)glycine or (2-/sup 14/C)glycine, indicating a randomization of label either in the primary or secondary metabolism of glycine. When whole cells were fed (1,2-/sup 14/C)glycine, cyanide and bicarbonate were the only radioactive extracellular products observed.

International Nuclear Information System (INIS)

Objective: To develop a method for RIA of transferrin without dilution of the serum sample. Methods: The measurable range was broadened through: Lowering of the specific radioactivity of the labelled antigen, increasing the concentration of the anti-serum and improve the curve fitting method. Results: The antiserum affinity was 3.82 x 10"-"1"0 mol/L, the specific radioactivity of the labelled antigen was 15.3 mg/mic, inter-assay error CV 5.38%, intra-assay error CV = 3.5% and the shape of the curve was good. For 30 normal control specimens, the mean TF content was 2.81 +- 0.972 mg/ml. Conclusion: Development of a method for RIA of TF without previous dilution of the test serum was of clinical value

British Library Electronic Table of Contents (United Kingdom)

Phenytoin (PHT), phenobarbital (PHB), lamotrigine (LTG), and topiramate (TPM) are some of the most widely used antiepileptic drugs (AEDs). Monitoring of their concentrations in serum is important for the treatment of epilepsy. A reference measurement procedure (RMP) for certification of PHT, PHB, LTG, and TPM in serum has been developed and critically evaluated. Isotopically labeled compounds of PHT, PHB, LTG, and TPM are used as internal standards for the four AEDs. The four drugs and their respective labeled internal standards are simultaneously extracted from serum using solid-phase extraction prior to reversed-phase liquid chromatography?tandem mass spectrometry (LC-MS/MS). Chromatographic separation was performed using a C18 column. Electrospray ionization (ESI) in the positive ion mo...

British Library Electronic Table of Contents (United Kingdom)

Summary Background- Treatments that achieve sustainable steroid-free clinical remission in Crohn-s disease are needed; however, long-term steroid-sparing efficacy data are limited. Aim- To evaluate steroid-sparing efficacy and the impact of steroid discontinuation on adverse events during treatment of Crohn's disease with adalimumab in the phase III randomised, double-blind 1-year CHARM trial and for an additional 2-years in its open-label extension ADHERE. Methods- Steroid-free remission and response and steroid-sparing (-50% steroid dose reduction) remission rates were evaluated over 3-years in patients who were taking corticosteroids at CHARM baseline. Results- Of 778 patients randomised in CHARM (including those who did not achieve clinical response to open-label induction therapy), 31...

CERN Document Server

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Science.gov (United States)

The authors describe an effect of suppression of invasion of the guinea pig eye conjunctiva and the HEp-2 epithelial cells by virulent Sh. flexneri bacilli, with a simultaneous administration of the same dose of avirulent shigella mutants, genetically connected with them. The data of morphological study and experiments with 3H-glucose labeled shigellae carried out on the cell species model indicated that the bacterial competition for the specific sites for absorption on the epithelial cells underlay the observed phenomenon. PMID:331774

Energy Technology Data Exchange (ETDEWEB)

Hyaluronate (HA) distribution patterns were examined in the cranial mesenchyme underlying the mesencephalic neural folds of mouse embryos maintained in roller tube culture. Using standard image-processing techniques, the digitized images of Alcian blue-stained or 3H-glucosamine-labeled sections digested with an enzyme specific for HA, were subtracted from adjacent, undigested sections. The resultant difference picture images (DPI) accurately depicted the distribution of stained or labeled HA within the cranial mesenchyme. 3H-glucosamine-labeled HA was distributed uniformly throughout the cranial mesenchyme as 12, 18, and 24 hr of culture. By contrast, the mesenchyme was uniformly stained with Alcian blue at 12 hr, but stain intensity decreased in the central regions of the mesenchyme at 18 and 24 hr. HA distribution patterns were also examined in the cranial mesenchyme of embryos cultured in the presence of ...

Energy Technology Data Exchange (ETDEWEB)

Vulnerability of 25-hydroxy-(26,27-{sup 3}H)vitamin D{sub 3} 3{beta}-N-(4-azido-2-nitrophenyl)glycinate, a photoaffinity analogue of 25-hydroxyvitamin D{sub 3} (25-OH-D{sub 3}) toward standard conditions of carboxymethylationin promoted the authors to synthesize 25-hydroxyvitamin D{sub 3} 3{beta}-3{prime}-(N-(4-azido-2-nitrophenyl)amino)propyl ether (25-ANE), a hydrolytically stable photoaffinity analogue of 25-OH-D{sub 3}, and 25-hydroxyvitamin D{sub 3} 3{beta}-3{prime}-(N-(4-azido-2-nitro-(3,5-{sup 3}H)phenyl)amino)propyl ether ({sup 3}H-25-ANE), the radiolabeled counterpart of 25-ANE competes for the 25-OH-D{sub 3} binding site in rat serum vitamin D binding protein (rDBP). On the other hand, UV exposure of a sample of purified rat DBP (rDBP), preincubated in the dark with {sup 3}H-25-ANE, covalently labeled the protein. However, very little covalent labeling was observed in the absence of UV light or in the presence of a large excess of ...

UK PubMed Central (United Kingdom)

The use of stable indole-3yl-acetic acid (IAA) labeled by 6 atoms of ¹³C allowed, after [¹³C]IAA treatment, simultaneous gas chromatography-mass spectrometry quantifications of...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Sulfate activation occurs in Euglena mitochondria the authors now find that the sulfate activating enzymes are absent from Euglena chloroplasts. Cells of mutant W/sub 10/BSmL lacking plastids also lack detectable sulfolipid (SL) when grown on /sup 35/SO/sub 4//sup 2 -/ indicating that SL is absent from the mitochondria and is exclusively in the plastids. Plastids alone will convert /sup 35/S-cysteine to /sup 35/SL in the presence of ATP and Mg/sup 2 +/; light is stimulatory. Under similar conditions, chloroplasts and mitochondria incubated together convert /sup 35/SO/sub 4//sup 2 -/ to plastid-localized /sup 35/SL but either organelle incubated alone fails to effect this conversion. Unlabeled cysteine blocks SL labeling from sulfate in the mixed incubation; since cysteine is formed from sulfate by Euglena mitochrondria, cysteine (and other compounds) may move from the mitochondrion to the chloroplast to provide the sulfo group for SL formation. Although ...

International Nuclear Information System (INIS)

Lead-203 [52.1 h, 279 (80.1%) KeV] has been recognized as a potentially useful tracer for tumor specific radiopharmaceuticals due to its favorable nuclear and chemical properties. This paper reports the cyclotron production of lead-203 and the labelling of monoclonal antibody B72.3, conjugated with 2-(p-isothiocyanatobenzyl)DOTA, with lead-203 in 30% yield. In vivo biodistribution and stability studies in mice are being conducted.

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The mechanisms of brown adipocyte proliferation and differentiation during cold acclimation (and/or adaptation to hyperphagia) have been studied by quantitative photonic radioautography. (/sup 3/H)thymidine was injected to warm-acclimated (25/sup 0/C) rats and to animals exposed to 5/sup 0/C for 2 days. Samples of interscapular brown adipose tissue were collected for quantitative analysis of mitotic frequencies at various periods of time (4 h-15 days) after the injection of (/sup 3/H)thymidine, the rats being maintained at the temperatures to which they were initially exposed. It was found that cold exposure for 2 days markedly enhanced mitotic activity in endothelial cells, interstitial cells, and brown preadipocytes rather than in fully differentiated brown adipocytes. The total tissue labeling index (percent of labeled nuclei) increased approx.70 times over control values. The authors now report that cellular labeling ...

International Nuclear Information System (INIS)

In developing new receptor-site specific brain imaging agents labeled with I-123 for single photon emission computed tomography (SPECT), an analog of raclopride, 3-["1"2"5I]-iodo-N-[(1-ethyl-2-pyrrolidinyl)-methyl]-2-hydroxy-6-me-thoxy benzamide (IBZM), was prepared using a method similar to that reported for raclopride and its derivatives. (author).

Energy Technology Data Exchange (ETDEWEB)

Positron emission tomography (PET) and magnetic resonance imaging (MRI) studies were performed on a case of neuro-Behcet's syndrome. In accordance with the clinical signs, FDG PET (using /sup 18/F-labeled 2-F-2'-desoxyglucose) revealed disseminated storage defects in the cerebrum and cerebellum. Focal regions of enhanced signal intensity were demonstrated in the parietal white matter of the cerebrum in T2-weighted images and in the brain stem by MRI. (orig.).

UK PubMed Central (United Kingdom)

Anti-TAG-72 monoclonal antibodies target the tumor-associated glycoprotein (TAG)-72 in various solid tumors. This study evaluated the use of anti-TAG-72 monoclonal antibodies, both murine CC49...Full Text Available

International Nuclear Information System (INIS)

The "1"3"1I-labelled monoclonal antibodies 791T/36 and 79IT/36 Fab fragments have been evaluated in the imaging of patients with colorectal cancer, with benign colorectal tumours, and with malignant tumours of other parts of the gastrointestinal tract. The results of clinical imaging have been carefully correlated with clinical findings and direct measurement of the preferential uptake of antibody by the tumours. (UK).

International Nuclear Information System (INIS)

This paper describes a method for measuring oil consumption in compressors. The oil is labelled with Zn 65. The consumed radioactive oil is retained in an activated carbon filter and its activity is measured continuously with a plastic scintillator detector. Measuring the oil consumption at a given operating levels takes about 15 minutes. At an oil consumption rate of about 1 g/h the measurement errors are of the order of +- 5%. (orig.).

Environmental Research Database

Objectives1) To establish a protocol for transfection of immortalised type I cells with SiRNA and its effect on cell viability; 2) To optimise silencing of genes involved in endocystosis using SiRNA and confirm successful transfection with western blotting; 3) To investigate the effect of gene silencing on uptake of flourescently-labelled latex nanoparticles.DescriptionTo investigate the mechanisms of particle uptake.

UK PubMed Central (United Kingdom)

Short term (10 min) influx of ⁸⁶Rb-labeled potassium into corn (Zea mays L. WF9 × M14) root segments was inhibited by La (NO₃)₃ or LaCl₃....Full Text Available

Energy Technology Data Exchange (ETDEWEB)

The affinity label 8-((4-bromo-2,3-dioxobutyl)thio)adenosine 5{prime}-triphosphate (8-BDB-TA-5{prime}-TP) reacts covalently with rabbit muscle pyruvate kinase, incorporating 2 mol of reagent/mol of enzyme subunit upon complete inactivation. Protection against inactivation is provided by phosphoenolpyruvate, K{sup +}, and Mn{sup 2+} and only 1 mol of reagent/mol of subunit is incorporated. The authors have now identified the resultant modified residues. After reaction with 8-BDB-TA-5{prime}-TP at pH 7.0, modified enzyme was incubated with ({sup 3}H)NaBH{sub 4} to reduce the carbonyl groups of enzyme-bound 8-BDB-TA-5{prime}-TP and to introduce a radioactive tracer into the modified residues. Following carboxymethylation and digestion with trypsin, the radioactive peptides were separated on a phenylboronate agarose column followed by reverse-phase high-performance liquid chromatography in 0.1% trifluoroacetic acid with an acetonitrile gradient. Gas-phase sequencing ...