UK PubMed Central (United Kingdom)

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor,...Full Text Available

International Nuclear Information System (INIS)

The effects of irradiation on microbiological controlling, nutrient and sensory qualities of packaged tofu (bean curd) stored at commercial condition. Results showed that D10 values of Listeria innocua and Samonella enteritidis inoculated in packaged tofu were 0.225 and 0.240kGy, respectively. Irradiation dose lower than 2.0kGy had no significant effects on content of crude protein and amino acid (p>0.05). ?-irradiation could decrease microbial in packaged tofu and 2.0kGy should be applied to ensure the hygienic quality of the products. (authors)

UK PubMed Central (United Kingdom)

Sectors of Zea mays cobs, with and without kernels were cultured in vitro in the presence and absence of fluridone. Cultured kernels, cob tissue, and embryos developed...Full Text Available

Norwegian Open Research Archives (NORA)

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International Nuclear Information System (INIS)

This study was carried out to evaluate the effects of irradiation (gamma irradiation at dose levels of 0.0, 2.5, 5.0, 10.0 or 20.0 kGy, He-Ne and their combination) on nutritive characteristics of Vicia faba L. seeds (Giza 843). Analyses included levels of anti-nutrients (total phenolic compounds, tannins, phytic acid, trypsin inhibitor and vicine), chemical composition (sugars, crude proteins, fat, dry matter and ash) and fatty acids profile. All treatments caused significant (P<0.05) decreases in the antinutrational factors under investigation. The reduction in the content of tannins and phenolic compounds were more pronounced with He-Ne alone or He-Ne in combination with gamma irradiation. At dose levels 10.0 and 20.0 kGy without or with combination of He-Ne were more efficiency in the reduction of phytic acid content by 24.3, 32.8, 34.5 and 45.5 %, respectively. Also, for trypsin inhibitor activity, the maximum inhibition was observed ...

UK PubMed Central (United Kingdom)

Acetolactate synthase (EC 4.1.3.18) activity was examined in maize (Zea mays L.) endosperm and embryos as a function of kernel development. When assayed using unpurified homogenates,...Full Text Available

CERN Document Server

In longitudinal and spatial studies, observations often demonstrate strong correlations that are stationary in time or distance lags, and the times or locations of these data being sampled may not be homogeneous. We propose a nonparametric estimator of the correlation function in such data, using kernel methods. We develop a pointwise asymptotic normal distribution for the proposed estimator, when the number of subjects is fixed and the number of vectors or functions within each subject goes to infinity. Based on the asymptotic theory, we propose a weighted block bootstrapping method for making inferences about the correlation function, where the weights account for the inhomogeneity of the distribution of the times or locations. The method is applied to a data set from a colon carcinogenesis study, in which colonic crypts were sampled from a piece of colon segment from each of the 12 rats in the experiment and the expression level of p27, an important cell cycle ...

International Nuclear Information System (INIS)

Crude N,N-dialkylcarbamoylmethylphosphonates and phosphine oxide extractants, and particularly crude dihexyl N,N-diethylcarbamoylmethylphosphonate and octylphenyl N,N-diisobutylcarbamoyl phosphine oxide, are purified by distilling the crude materials in a thin film evaporator. Preferably, the crude materials are reacted with concentrated hydrochloric acid and then with aqueous sodium hydroxide prior to distillation in the thin film evaporator to hydrolyze impurities contained in them. The purified extractants are useful for extracting actinides and lanthanides from liquid waste streams. (author).

Science.gov (United States)

Phytoremediation has been identified as a potentially environmentally friendly and cost effective technique for the treatment of contaminated soil. However, phytoremediation has an unknown mechanism. In this study, we focus on the effects of the cultivation of Italian ryegrass on the soil microbes collected at oil showings, which were expected to have a variety of crude oil degradable microorganisms. We evaluated the number of crude oil degradable microorganism, microbial activity, microflora using the PCR-DGGE method and the change in the concentration of crude oil in the soil. The results indicated that the microflora was affected by the cultivation of Itarian ryegrass, and that the microbial activity and the number of crude oil degradable microorganisms were also improved by the cultivation. Moreover, the concentration of crude oil in the rhizosphere soil decreased significantly ...

Energy Technology Data Exchange (ETDEWEB)

The interpretation of soil amelioration experiments with peanuts is made difficult by the unpredictibility of the crop and by the many factors altered when ameliorating acid soils. The present study was conducted to investigate the effects of lime and gypsum applications on peanut kernel yield via the three first order yield components, pods per ha, kernels per pod, and kernel mass. On an acid medium sandy loam soil (typic Plinthustult), liming resulted in a highly significant kernel yield increase of 117% whereas gypsum applications were of no significant benefit. As indicated by path coefficient analysis, an increase in the number of pods per ha was markedly more important in increasing yield than an increase in either the number of kernels per pod or kernel mass. Furthermore, exch. Al was found to be particularly detrimental to pod number. It was postulated ...

International Nuclear Information System (INIS)

In connection with some applications of asymptotic numbers and asymptotic functions, proposed by Khr.Khristov, the problem of describing subsets of asymptotic numbers closed with respect to algebraic operations arises. The algebraic operations with asymptotic numbers are defined by classes of their representatives. All trivial or noncharacteristic solutions are avoided. A procedure for constructing sets of elements closed under action of an algebraic operation or a combination of two or more of them is given. It turns out that the closed sets are given by their kernels, the last being the minimal subsets which generate the whole set by the introduced algebraic operations. It is proved that such kernels exist always. . The closed sets are described by their correspondence with the kernels. (S.P.).

British Library Electronic Table of Contents (United Kingdom)

In this study, a two-stage support-vector-regression optimization model (TSOM) is developed for the planning of municipal solid waste (MSW) management in the urban districts of Beijing, China. It represents a new effort to enhance the analysis accuracy in optimizing the MSW management system through coupling the support-vector-regression (SVR) model with an interval-parameter mixed integer linear programming (IMILP). The developed TSOM can not only predict the city's future waste generation amount, but also reflect dynamic, interactive, and uncertain characteristics of the MSW management system. Four kernel functions such as linear kernel, polynomial kernel, radial basis function, and multi-layer perception kernel are chosen based on three quantitative simulation performance criteria [i.e....

UK PubMed Central (United Kingdom)

Kernels of Zea mays on an intact plant accumulate indole-3-acetic acid (IAA) at the rate of 190 ng g-1 fresh weight h-1. Of the IAA synthesized, 97% is in the esterified form and less than 3% remains...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Experiments were conducted to confirm the isotropic spatial resolution of multislice CT with a 0.5 mm slice thickness. Isotropic spatial resolution means that the spatial resolution in the transaxial plane (X-Y plane) and that in the longitudinal direction (Z direction) are equivalent. To obtain point spread function (PSF) values in the X-Y-Z directions, three-dimensional voxel data were obtained by helical scanning of a bead phantom. The modulation transfer function (MTF) values were then obtained by three-dimensional Fourier transform of the PSF. Evaluation of the spatial resolution in the X-Y-Z directions by the MTF values showed that the spatial resolution in the Z direction does not depend on the reconstruction kernel used. It was also found that the spatial resolution in the Z direction, as compared with that in the X-Y plane, is superior with the standard kernel for the abdomen and is inferior with the high-definition ...

J-STORE (Japan)

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British Library Electronic Table of Contents (United Kingdom)

Surveys were carried out in 2006 and 2007 in Umbria (central Italy) to evaluate the presence of mycotoxigenic fungi and mycotoxins in maize grain sampled at harvest. Fusarium spp., were the most abundant species detected in maize kernels, followed by Aspergillus species of sections Flavi and Nigri and by Penicillium spp. Among Fusarium species, F. verticillioides was the most prevalent species, as detected by PCR directly on the kernels and on the fungi isolated from the kernels, followed by F. proliferatum and F. subglutinans. Fumonisins were the predominant mycotoxins with values, on average, of 4.3 and 5.7mgkg-1, in 2006 and 2007, respectively, with a maximum of 76.3mgkg-1 in the second year. Deoxynivalenol ranged from 0.2 to 3.98mgkg-1 in 2006 (average 1.04mgkg-1) and from undetectable...

Energy Technology Data Exchange (ETDEWEB)

Capital investment in extra heavy crude production is expected to increase from $6 billion to $25 billion by 2005. In response to this forecast, producers are focusing their efforts on improving the quality of extra heavy crude right at the production sites to reduce their dependence on diluent, reduce pipelining costs, and to receive a higher return for their investment. Their efforts would also minimize environmental impacts at production sites. This slide presentation also proposed a solution to the problem associated with stability and incompatibility of thermal upgrading by taking advantage of the inherent incompatibility of extra heavy crudes and paraffinic diluents and using diluents to perform selective decarbonization (or the partial removal of asphaltene) to reduce heavy crude viscosity and density. A pilot-scale test has been conducted and a bench-scale unit will be carried out in Fall 2000.

UK PubMed Central (United Kingdom)

Governments are increasingly concerned to compare the quality and effectiveness of healthcare interventions but find this a complex matter. Crude hospital statistics can be dangerously misleading and...Full Text Available

UK PubMed Central (United Kingdom)

In germinating oil palm (Elaeis guineensis var D × P) seedling, an active lipase was present in the shoot but absent from both the kernel and the haustorium. It has an optimum...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundGene regulation is a key mechanism in higher eukaryotic cellular processes. One of the major challenges in gene regulation studies is to identify regulators affecting the...Full Text Available

UK PubMed Central (United Kingdom)

Indole-3-acetyl-myo-inositol occurs in both the kernel and vegetative shoot of germinating Zea mays seedlings. The effect of a gravitational stimulus on the transport...Full Text Available

Science.gov (United States)

The plerocercoid stage of the tapeworm Spirometra mansonoides produces a functional analog of human growth hormone (hGH). Among the similarities between plerocercoid growth factor (PGF) and hGH is competition for the same receptors on rabbit liver membranes. To take advantage of this characteristic in a purification scheme for PGF, rabbit liver microsomes were solubilized in Triton X-100 and the hGH receptors were purified over an hGH affinity column. The purified receptors from six rabbit livers were coupled to Affi-Gel-10 to create a receptor affinity column which was used to purify PGF. Chromatography of crude PGF over the receptor column resulted in a 1044 fold increase in specific activity. SDS-PAGE in the presence of 2-mercaptoethanol showed that the affinity-purified PGF contained three protein bands with apparent Mrs of 27.5 K, 22 K, and 16.7 K. Injections of the partially-purified PGF into hypophysectomized rats produced a ...

International Nuclear Information System (INIS)

Ten Holstein steers (579 kg average body weight) were fed either alfalfa hay (12.2% crude protein) or a 90% concentrate diet to supply 14.2 or 25.2 Mcal ME respectively for a minimum of 28 days. Samples of rumen epithelial tissue were removed at slaughter from the anterior ventral sac, washed free of feed particles and transported to the laboratory in oxygenated Krebs-Ringer bicarbonate buffer (KRB; pH 7.4). Papillae were weighed (100-200 mg) in triplicate into flasks containing 3 ml KRB with 1 mM glutamine or 5 mM glucose and acetate (50 mM), propionate (25 mM), butyrate (15 mM), lactate (1 mM) and glucose (5 mM) or glutamine (1 mM) as competing substrates. A parallel set of flasks contained 1 or .5 #mu#Ci of [U-"1"4C]-glutamine or glucose respectively for "1"4CO_2 production. There were no interactions with dietary energy intake and substrate addition. Increasing the dietary energy intake increased (P < .01) rates of uptake, "1"4CO_2 ...

International Nuclear Information System (INIS)

The radiative transport problem is cast in integral form using a transport kernel. The transport kernel has an explicit representation in terms of a Feynman Path Integral over all paths between selected points in a volume. This representation is setup in detail. Numerical evaluation of this Path Integral is formulated with a Frenet-Serret based procedure for generating valid random paths, and with a numerical evaluation of the weight for each valid path. Very early sanity checks of a numerical implementation are reported. Approaches to optimization are identified. (authors)

Energy Technology Data Exchange (ETDEWEB)

It may be necessary to reduce microbiological contamination of crude drugs (medicinal plants or their parts like roots, leaves, flowers). This can be done by treating the drugs with ionizing radiation. Meethods for detection of such an irradiation were developed. It could be pointed out that measurements of luminescence, viscosity and electron spin resonance were suitable for specific drugs, but not for all drugs. (orig.).

Science.gov (United States)

Copolymers of a 1,2 epoxy alkane and a cyclic carboxylate compound of the class consisting of dicarboxylic acid anhydrides, preferably maleic anhydride or a hydrocarbyl substituted succinic anhydride and a beta lactone, said copolymers having C/sub 10/ to C/sub 50/, preferably C/sub 20/ to C/sub 40/, linear, pendant hydrocarbon chains are flow improvers in residual and crude oils.

Energy Technology Data Exchange (ETDEWEB)

To investigate the future problem of oil field operation in the North Sea, the latest trend of the resources and development/production of the oil fields in the North Sea in British and Norwegian territories are outlined. Recoverable crude oil reserves, potential additional crude oil reserves, and undiscovered recoverable reserves of Britain, as well as probable total potential crude oil reserves in British continental shelf are put in order. Outlines of development and production of crude oil are described. The petroleum and gas development by Norway in 1996 is arranged, and outlines of the reserves, development, and petroleum/gas production are given. As the topics relating to the North Sea in 1996, reports are made on: the effective use of existing infrastructure surplus capacity; dealing with economic efficiency; challenge to abysmal development; applied instances of floating production; storage and ...

DEFF Research Database (Denmark)

Udgivelsesdato: 2008/5

UK PubMed Central (United Kingdom)

Reversion-induced LIM protein (RIL) is a member of the ALP (actinin-associated LIM protein) subfamily of the PDZ/LIM protein family. RIL serves as an adaptor protein and seems to regulate cytoskeletons....Full Text Available

Energy Technology Data Exchange (ETDEWEB)

A state-of-the-art review and assessment of storage effects on crude oil and product quality was undertaken through a literature search by computer accessing several data base sources. Pertinent citations from that literature search are tabulated for the years 1980 to the present. This 1990 revision supplements earlier reviews by Brinkman and others which covered stability publications through 1979 and an update in 1983 by Goetzinger and others that covered the period 1952--1982. For purposes of organization, citations are listed in the current revision chronologically starting with the earliest 1980 publications. The citations have also been divided according to primary subject matter. Consequently 11 sections appear including: alternate fuels, gasoline, distillate fuel, jet fuel, residual fuel, crude oil, biodegradation, analyses, reaction mechanisms, containment, and handling and storage. Each section contains a brief narrative followed by ...

International Nuclear Information System (INIS)

In the convolution/superposition method of photon beam dose calculations, inhomogeneities are usually handled by using some form of scaling involving the relative electron densities of the inhomogeneities. In this paper the accuracy of density scaling as applied to primary electrons generated in photon interactions is examined. Monte Carlo calculations are compared with density scaling calculations for air and cork slab inhomogeneities. For individual primary photon kernels as well as for photon interactions restricted to a thin layer, the results can differ significantly, by up to 50%, between the two calculations. However, for realistic photon beams where interactions occur throughout the whole irradiated volume, the discrepancies are much less severe. The discrepancies for the kernel calculation are attributed to the scattering characteristics of the electrons and the consequent oversimplified modeling used in the density scaling method. A ...

CERN Document Server

We consider a family of chiral non-Hermitian Gaussian random matrices in the unitarily invariant symmetry class. The eigenvalue distribution in this model is expressed in terms of Laguerre polynomials in the complex plane. These are orthogonal with respect to a non-Gaussian weight including a modified Bessel function of the second kind, and we give an elementary proof for this. In the large $n$ limit, the eigenvalue statistics at the spectral edge close to the real axis are described by the same family of kernels interpolating between Airy and Poisson that was recently found by one of the authors for the elliptic Ginibre ensemble. We conclude that this scaling limit is universal, appearing for two different non-Hermitian random matrix ensembles with unitary symmetry. As a second result we give an equivalent form for the interpolating Airy kernel in terms of a single real integral, similar to representations for the asymptotic ...

British Library Electronic Table of Contents (United Kingdom)

Energy use patterns and utilisation efficiencies in 40 factories producing palm kernel oil (PKO) in southwestern part of Nigeria were studied. The factories were stratified into small, medium and large scale categories based on the mode of operations and production capacities. Questionnaires were administered on the factories to obtain historical data on petrol, diesel and electricity consumption and PKO production outputs for seven years (1998-2004). Energy use efficiency indicators employed include: energy intensity (EI), energy cost per unit product (EC/P), energy ratio (ER), food energy ratio (FER) and percentage oil yield by weight. Results of the study indicated that averagely, 0.58, 0.53 and 0.74GJ/103l of PKO were needed in the small, medium and large PKO factories, respectively. T...

Science.gov (United States)

Petroleum contamination of groundwater is widely recognized as a serious environmental problem. Toluene (methylbenzene) occurs naturally in crude oil and is commonly found as a contaminant in the subsurface as a result of waste disposal and storage activities. Biological transf...

UK PubMed Central (United Kingdom)

Assimilatory nitrate reductase activity (NRA) in crude spinach leaf (Spinacia oleracea) extracts undergoes rapid changes following fluctuations in photosynthesis brought about by changes...Full Text Available

International Nuclear Information System (INIS)

Over a 5-year period (1976-1980) 499 patients with non-seminomatous testicular germ cell tumours were included in the Danish Testicular Carcinoma Study (DATECA). The 3-year crude survival for patients in clinical stages I, II and III was 91, 77 and 45 per cent, respectively. In stage I the relapse-free survival for patients given radiation combined with bleomycin and vincristine was significantly higher than that for patients given radiation alone. No difference in the crude survival for patients given radiation combined with bleomycin and vincristine was significantly higher than that for patients given radiation alone. No difference in the crude survival was observed. For stage II patients maintenance chemotherapy following radiation combined with bleomycin and vincristine did not improve relapse-free or crude survival. The survival of patients with non-seminomatous tumours improved significantly ...

UK PubMed Central (United Kingdom)

Infection due to multidrug resistance pathogens is difficult to manage due to bacterial virulence factors and because of a relatively limited choice of antimicrobial agents. Thus, it is imperative to...Full Text Available

UK PubMed Central (United Kingdom)

Leupeptin, a tripeptide inhibitor of some proteinases, was shown previously to maintain the stability of several enzymes (isocitrate lyase, fumarase, and catalase) in crude extracts of castor bean endosperm....Full Text Available

UK PubMed Central (United Kingdom)

The soil-isolated strain XP was identified as Rhodococcus erythropolis. R. erythropolis XP could efficiently desulfurize benzonaphthothiophene, a complicated model sulfur compound that...Full Text Available

Science.gov (United States)

Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding protein that is

UK PubMed Central (United Kingdom)

Many mutations associated with retinal degeneration lead to the production of misfolded proteins by cells of the retina. Emerging evidence suggests that these abnormal proteins cause cell death...Full Text Available

CERN Document Server

Standard methods in non-linear analysis are used to show that there exists a parabolic branching of solutions of the Lichnerowicz-York equation with an unscaled source. We also apply these methods to the extended conformal thin sandwich formulation and show that if the linearised system develops a kernel solution for sufficiently large initial data then we obtain parabolic solution curves for the conformal factor, lapse and shift identical to those found numerically by Pfeiffer and York. The implications of these results for constrained evolutions are discussed.

International Nuclear Information System (INIS)

Both the accuracy and real-time are quite strictly required for the RCS thermal-hydraulic model of the simulation of nuclear power station. So, a large amount of reasonable simplifications should be carried on. The author introduces the RCS thermal-hydraulic model of the full scope simulator of Daya Bay Nuclear Power Station, and emphasizes on the fundamental hypotheses and computation method of its kernel part--DEFI programme.

International Nuclear Information System (INIS)

The convolution/superposition calculations for radiotherapy dose distributions are traditionally performed by convolving polyenergetic energy deposition kernels with TERMA (total energy released per unit mass) precomputed in each voxel of the irradiated phantom. We propose an alternative method in which the TERMA calculation is replaced by random sampling of photon energy, direction and interaction point. Then, a direction is randomly sampled from the angular distribution of the monoenergetic kernel corresponding to the photon energy. The kernel ray is propagated across the phantom, and energy is deposited in each voxel traversed. An important advantage of the explicit sampling of energy is that spectral changes with depth are automatically accounted for. No spectral or kernel hardening corrections are needed. Furthermore, the continuous sampling of photon direction allows us to model sharp changes in ...

UK PubMed Central (United Kingdom)

Resistance (R) protein mediated recognition of pathogen avirulence effectors triggers signaling that induces a very robust form of species-specific immunity in plants. The soybean Rpg1-b protein mediates...Full Text Available

UK PubMed Central (United Kingdom)

Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane...Full Text Available

KoreaScience (Korea)

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Energy Technology Data Exchange (ETDEWEB)

An assay method for ATP sulfurylase is presented which employs Na/sub 2/(35)SO/sub 4/ as a substrate and measures the production of labeled adenosine 5'-phosphosulfate and 3'-phosphoadenosine 5'-phosphosulfate by low-voltage, hanging paper strip electrophoresis. The method is applicable to crude bacterial or mammalian extracts and accurately measures picomole amounts of product(s). Na/sub 2/(/sup 75/)SeO/sub 4/ can also be employed as a substrate, if the unstable radioactive product, adenosine 5'-phosphoselenate, is converted to elemental /sup 75/Se degrees by inclusion of reduced glutathione in the reaction mixture. The same paper strip electrophoretic technique can then be used to separate /sup 75/Se degrees from the radiolabeled substrate. The method also has utility for measuring any direct reduction by crude microbial extracts of radioactive selenate to selenite, independent of ATP sulfurylase.

Energy Technology Data Exchange (ETDEWEB)

Biocatalytic Desulfurization (BDS) represents an alternative approach to the reduction of sulfur in fossil fuels. The objective is to use bacteria to selectively remove sulfur from petroleum and middle distillate fractions, without the concomitant release of carbon. Recently, bacteria have been developed which have the ability to desulfurize dibenzothiophene (DBT) and other organosulfur molecules. These bacteria are being developed for use in a biocatalyst-based desulfurization process. Analysis of preliminary conceptual engineering designs has shown that this process has the potential to complement conventional technology as a method to temper the sulfur levels in crude oil, or remove the recalcitrant sulfur in middle distillates to achieve the deep desulfurization mandated by State and Federal regulations. This paper describes the results of initial feasibility studies, sensitivity analyses and conceptual design work. Feasibility studies with various ...

Energy Technology Data Exchange (ETDEWEB)

Sasol Limited's 1986 annual report for the year ended June 30, 1986 reported an increase in profit of 14.8% for the year. Financial measures taken in order to counteract the effect of the lower oil prices are described. Operating results and technical modifications of the Sasol One, Two, and Three plants are discussed. Sasol believes that crude oil prices will remain volatile for some time and will fluctuate between $10 and $18 per barrel. A steady increase in crude oil prices will then occur in the 1990's. As a result of the conservative financial policy of the past few years, Sasol is well equipped to cope with low fuel prices in the short and medium term.

British Library Electronic Table of Contents (United Kingdom)

We have recently introduced several important improvements in the measurement of distillation curves for complex fluids. This new method is a significant improvement over current approaches, with features including a composition-explicit data channel for each distillate fraction (for both qualitative and quantitative analysis), trace chemical analysis of each distillate fraction, and a corrosivity assessment of each distillate fraction. In the present contribution, we present the application of the advanced distillation curve method to a bio-derived crude oil prepared from swine manure. One motivation behind the work was to precisely measure the distillation curve of the oil derived from swine manure with our advanced distillation apparatus and compare the bio-derived oil to traditional pe...

International Nuclear Information System (INIS)

A method has been developed for calibration of CT-numbers to volumetric electron density distributions using tissue substitutes of known elemental composition and experimentally determined electron density. This information have been used in a dose calculation method based on photon and electron interaction processes. The method utilizes a convolution integral between the photon fluence matrix and dose distribution kernels. Inhomogeneous media are accounted for using the theorems of Fano and O'Connor for scaling dose distribution kernels in proportion to electron density. For clinical application of a calculated dose plan, a method for prediction of accelerator output have been developed. The methods gives the number of monitor units that has to be given to obtain a certain absorbed dose to a point inside an irregular, inhomogeneous object. The method for verification of dose distributions outlined in this study makes it possible to exclude the ...

British Library Electronic Table of Contents (United Kingdom)

A convolution/superposition method is proposed for use with primary and scatter dose kernels formed for energy bins of X-ray spectra reconstructed as a function of off-axis distance. It should be noted that the number of energy bins is usually about ten, and that the reconstructed X-ray spectra can reasonably be applied to media with a wide range of effective Z numbers, ranging from water to lead. The study was carried out for 10-MV X-ray doses in water and thorax-like phantoms with the use of open-jaw-collimated fields. The dose calculations were made separately for primary, scatter, and electron contamination dose components, for which we used two extended radiation sources: one was on the X-ray target and the other on the flattening filter. To calculate the in-air beam intensities at po...

CERN Document Server

A theory of nonunitary-invertible as well as unitary canonical transformations is formulated in the context of Weyl's phase space representations. Exact solutions of the transformation kernels and the phase space propagators are given for the three fundamental canonical maps as fractional-linear, gauge and contact (point) transformations. Under the nonlinear maps a phase space representation is mapped to another phase space representation thereby extending the standard concept of covariance. This extended covariance allows Dirac-Jordan transformation theory to naturally emerge from the Hilbert space representations in the Weyl quantization.

UK PubMed Central (United Kingdom)

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

UK PubMed Central (United Kingdom)

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...Full Text Available

UK PubMed Central (United Kingdom)

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

UK PubMed Central (United Kingdom)

The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis. Of the 261 proteins scored, 68 (26%) show dramatic changes in...Full Text Available

UK PubMed Central (United Kingdom)

A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands...Full Text Available

UK PubMed Central (United Kingdom)

We have used the two-electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK1 channels by protein-tyrosine phosphatase (PTP) and protein-tyrosine...Full Text Available

UK PubMed Central (United Kingdom)

A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts...Full Text Available

UK PubMed Central (United Kingdom)

Protein modifications such as phosphorylation are often studied by two-dimensional gel electrophoresis since the perturbation in the protein’s pI value is readily detected by this method....Full Text Available

UK PubMed Central (United Kingdom)

The Protein Data Bank in Europe (PDBe; pdbe.org) is actively involved in managing the international archive of biomacromolecular structure data as one of the partners in the Worldwide Protein Data Bank...Full Text Available

UK PubMed Central (United Kingdom)

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this...Full Text Available

UK PubMed Central (United Kingdom)

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

UK PubMed Central (United Kingdom)

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

UK PubMed Central (United Kingdom)

Ethylene rapidly and transiently up-regulates the activity of several monomeric GTP-binding proteins (monomeric G proteins) in leaves of Arabidopsis as determined by two-dimensional gel electrophoresis...Full Text Available

UK PubMed Central (United Kingdom)

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

UK PubMed Central (United Kingdom)

Tooth enamel biomineralization is mediated by enamel proteins synthesized by ameloblast cells. Two classes of proteins have been described: enamelins and amelogenins. In lower vertebrates the absence...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe fast growing Protein Data Bank contains the three-dimensional description of more than 45000 protein- and nucleic-acid structures today. The large majority of the data...Full Text Available

UK PubMed Central (United Kingdom)

Alpha-hemoglobin-stabilizing protein (AHSP) is an erythroid-specific protein that acts as a molecular chaperone for the free α chains of hemoglobin. Evidence strongly suggests...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

China is continuing to extract oil from kerogen shale despite having a crude production capacity of 100 Mt a year. Among the reasons for this policy are the country's rich oil shale resources, comparatively mature extraction techniques, and a comprehensive utilization of the shale that has proved to be economically profitable. China has oil shale deposits of 340 Gt, equivalent to 24.5 Gt of shale oil, to rank fourth in the world after the U.S., Brazil, and the Soviet Union.

Energy Technology Data Exchange (ETDEWEB)

Discussion is presented of world crude steel production, the Japanese steel industry and its expectations of the Canadian coal industry. It is concluded that the Canadian coal industry should pay attention to the trend in customer's requirements regarding coal quality, maintain price competitiveness in the international market place, and remain flexible with regard to the market trend.

Energy Technology Data Exchange (ETDEWEB)

The UK's reserves of crude oil could last another 300 years. This is the result it is hoped to obtain from tests currently being carried out in British Coal's liquefaction project of which the objective is to produce a high quality liquid fuel from coal. 1 fig.

Energy Technology Data Exchange (ETDEWEB)

We have recently introduced several important improvements in the measurement of distillation curves for complex fluids. The modifications include a composition-explicit data channel for each distillate fraction (for both qualitative and quantitative analysis) and corrosivity assessment of each distillate fraction. The composition-explicit information is achieved with a new sampling approach that allows precise qualitative as well as quantitative analyses of each fraction, on the fly. We have applied the new method to a variety of fluids, including simple n-alkanes, rocket propellant, gasoline, jet fuels, and a hydrocarbon fluid made corrosive with dissolved hydrogen sulfide. In the current contribution, we present the application of the advanced distillation curve method to two samples of crude oil. A primary motivation behind the work is to precisely measure the distillation curves of these oils using our advanced distillation apparatus; these low uncertainty ...

UK PubMed Central (United Kingdom)

The receptor-associated protein (RAP) is a molecular chaperone that binds tightly to certain newly synthesized LDL receptor family members in the endoplasmic reticulum (ER) and facilitates their delivery...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

UK PubMed Central (United Kingdom)

We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter...Full Text Available

Science.gov (United States)

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

UK PubMed Central (United Kingdom)

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

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Energy Technology Data Exchange (ETDEWEB)

Biological cells of the nitrogen fixating bacteria Xanthobacter autotrophicus GZ 29 have been cultivated with 57-Fe, and from the nitrogenase of these cells the 57-Fe marked molybdenum-iron-protein is extracted. The Moessbauer spectra of this enzyme show that the structure of the iron cluster is similar to the structure in the molybdenum-iron-proteins of Azotobacter vinelandii, Klebsiella pneumoniae or Clostridium pasteurianum.

KoreaScience (Korea)

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KoreaScience (Korea)

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KoreaScience (Korea)

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KoreaScience (Korea)

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UK PubMed Central (United Kingdom)

Protein bodies within the endosperm of castor bean (Ricinus communis L. cv. Hale) seeds arise from numerous small vacuoles which progressively become filled with storage protein, of...Full Text Available

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International Nuclear Information System (INIS)

In a series of experiments carried out on young and adult rabbits the effect of isocaloric low protein diets containing 4% or 8% protein compared with a diet containing 21% protein on Obeliscoides cuniculi infection was studied. The pathogenesis, resistance and local immunity were assessed after single infections with 10,000 larvae or reinfection with 5000 larvae. Live weight gain was reduced in young and adult rabbits fed the low protein diets, but the establishment of parasites was not substantially influenced by protein deprivation. However, development of worms in the histotrophic phase and parasite fecundity were impaired in association with the low protein diet. Moreover, mild anaemia as well as changes in the mucosal immune response as a result of infection were related to the level of dietary protein. (author). 30 refs, 6 figs, 5 ...

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TC inhibits protein synthesis in E. coli by interfering with aminoacyl-tRNA binding to the ribosomal A site, and there is strong evidence that such inhibition results from TC binding to a high affinity site on the 30S subunit. The SPORE approach has been used to define those 30S proteins that are potentially important for high affinity TC binding, measured as the (/sup 3/H)-TC co-sedimenting with the reconstitution particle through a sucrose density gradient. Reverse phase-HPLC has been used both to prepare ribosomal proteins and to analyze the protein content of reconstituted particles. The results obtained so far show that protein S7, as well as some proteins linked to S7 in the 30S assembly map, are important for TC binding, whereas other ribosomal proteins are not. These results are in very good accord with their earlier photoaffinity ...

British Library Electronic Table of Contents (United Kingdom)

In this article, self-organizing-map-based video object segmentation is proposed, assuming that either Y-quantification or HSV-quantification can be systematically selected. Given a video sequence, the value of the probability density function for each component value is calculated according to a kernel estimation at the first frame. Some areas randomly chosen from the background are then examined, using each component value, to judge whether or not they include the target object. The quantification is determined so that the frequency of occurrence of false extractions can be reduced. The data presented to the maps are generated based on the selected quantification. Experimental results show that the proposed method recognizes the target object well.

Energy Technology Data Exchange (ETDEWEB)

We report on the improvement of Critical Dimension (CD) linearity on a photomask by applying the concept of process proximity correction to a laser lithographic process used for fabrication of photomasks. Rule-based Laser Process proximity Correction (LPC) was performed using an automated optical proximity correction tool and we obtained dramatic improvement of CD linearity on a photomask. A study on model-based LPC was executed using a two-Gaussian kernel function and we extracted model parameters for the laser lithographic process by fitting the model-predicted CD linearity data with measured ones. Model-predicted bias values of isolated space (I/S), Arrayed Contact (A/C) and Isolated Contact (I/C) were in good agreement with those obtained by the nonlinear curve-fitting method used for the rule-based LPC.

International Nuclear Information System (INIS)

Peculiarities of Kurchatov Institute WWR-2 and TR research reactors spent fuel treating and transportation for radiochemical processing are stated. Spent fuels were performed as fuel assemblies of different forms and containing similar fuel elements: EhK-10 with 10% enrichment UO2-Mg fuel kernels or S-36 with 36% enrichment U-Al alloys. Spent fuel storage conditions are described. Features of developed procedures for identification of fuel assemblies by type of fuel elements are given. Transport package TUK-19 for loading and transportation of spent fuel for processing was chosen. Details of spent fuel loading in TUK-19 that is conducted by personnel under protective sheet of water in special reclaim volume are described

CERN Document Server

We consider various modifications of the Weyl-Moyal star-product, in order to obtain a finite range of nonlocality. The basic requirements are to preserve the commutation relations of the coordinates as well as the associativity of the new product. We show that a modification of the differential representation of the Weyl-Moyal star-product by an exponential function of derivatives will not lead to a finite range of nonlocality. We also modify the integral kernel of the star-product introducing a Gaussian damping, but find a nonassociative product which remains infinitely nonlocal. We are therefore led to propose that the Weyl-Moyal product should be modified by a cutoff like function, in order to remove the infinite nonlocality of the product. We provide such a product, but it appears that one has to abandon the possibility of analytic calculation with the new product.

British Library Electronic Table of Contents (United Kingdom)

During the last few decades, accuracy in photon and electron radiotherapy has increased substantially. This is partly due to enhanced linear accelerator technology, providing more flexibility in field definition (e.g. the usage of computer-controlled dynamic multileaf collimators), which led to intensity modulated radiotherapy (IMRT). Important improvements have also been made in the treatment planning process, more specifically in the dose calculations. Originally, dose calculations relied heavily on analytic, semi-analytic and empirical algorithms. The more accurate convolution/superposition codes use pre-calculated Monte Carlo dose "kernels" partly accounting for tissue density heterogeneities. It is generally recognized that the Monte Carlo method is able to increase accuracy even furt...

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The adoption of a cogenerator integrated system is described. The dry kernel, a woody residue of olive pressing, is produced in very large quantities and could therefore be used in high pressure condensing-extraction team turbines to supply on-site electricity and steam needs. This system is becoming more and more interesting since it produces an excess of electricity which could be sold, e.g. to the electric utility. Such a cogeneration system is discussed in terms of efficiency, capital cost, near-time commercial viability and power-to-fuel costs. The economical analysis is presented for olive press-cake processing plants and can be extrapolated to biomass-based industries having the same characteristics. An energy audit has been carried out on 16 plants. (Author)

CERN Document Server

Recently, applying the novel data mining techniques for evaluating enterprise financial distress has received much research alternation. Support Vector Machine (SVM) and back propagation neural (BPN) network has been applied successfully in many areas with excellent generalization results, such as rule extraction, classification and evaluation. In this paper, a model based on SVM with Gaussian RBF kernel is proposed here for enterprise financial distress evaluation. BPN network is considered one of the simplest and are most general methods used for supervised training of multilayered neural network. The comparative results show that through the difference between the performance measures is marginal; SVM gives higher precision and lower error rates.

International Nuclear Information System (INIS)

We present a new relativistic bound-state formalism for two interacting Fermi-Dirac particles. The kernel of the integral equation for the bound-state system is generated by summing Feynman scattering amplitudes and multiplying by a bound-state amplitude. The method is illustrated through calculations of the hyperfine and fine splittings of positronium up to order #alpha#"5. Our calculations of the one-loop contributions are carried out in the explicitly covariant Feynman gauge. We also present new results for the hyperfine and fine splittings in positronium to order #alpha#"5 for arbitrary principal quantum number n, which are easily obtained owing to the virtue of conceptual and calculational simplicity of our formalism. In addition, we present the one-loop renormalization scheme in our formalism. (author).

CERN Document Server

In this paper we deal with a new class of Clifford algebra valued automorphic forms on arithmetic subgroups of the Ahlfors-Vahlen group. The forms that we consider are in the kernel of the operator $D \\Delta^{k/2}$ for some even $k \\in {\\mathbb{Z}}$. They will be called $k$-holomorphic Cliffordian automorphic forms. $k$-holomorphic Cliffordian functions are well equipped with many function theoretical tools. Furthermore, the real component functions have also the property that they are solutions to the homogeneous and inhomogeneous Weinstein equation. This function class includes the set of $k$-hypermonogenic functions as a special subset. While we have not been able so far to propose a construction for non-vanishing $k$-hypermonogenic cusp forms for $k \

Science.gov (United States)

Extensive laboratory and industrial tests are being conducted at the Western Siberian Iron and Steel Works (I and SW) on the adoption of preheating of coking. The present article describes the results of laboratory investigations of the effect of the free oxygen content in the gaseous heat carrier and the final charge preheating temperature on the technological properties of the charge and the quality of the blast furnace coke, as well as the yield and quality of the coking products: 1. An increase in the free oxygen concentration in the heat carrier to 8% degrades the strength properties of the coke - the crushability and abradability are increased. 2. Charge preheating to 200 to 250/sup 0/C, even with a low oxygen concentration in the heat carrier, is accompanied by degradation of the physical and mechanical properties of the coke. 3. The optimal temperature of charge preheating before coking at the Western Siberia I and SW was found to be in the 150 to 200/sup 0/C range, and the ...

Energy Technology Data Exchange (ETDEWEB)

In the past three years, we followed the work plan as we suggested in the proposal and made every efforts to fulfill the project objectives. Based on our large amount of creative and productive work, including both of experimental and theoretic aspects, we received important technical breakthrough on naphthenic acid removal process and obtained deep insight on catalytic decarboxylation chemistry. In detail, we established an integrated methodology to serve for all of the experimental and theoretical work. Our experimental investigation results in discovery of four type effective catalysts to the reaction of decarboxylation of model carboxylic acid compounds. The adsorption experiment revealed the effectiveness of several solid materials to naphthenic acid adsorption and acidity reduction of crude oil, which can be either natural minerals or synthesized materials. The test with crude oil also received promising results, which can be potentially ...

Energy Technology Data Exchange (ETDEWEB)

The authors investigated the effect of T-2 toxin on rat liver mitochondrial protein synthesis. Isolated rat liver mitochondria were supplemented with an S-100 supernatant from rat liver and an external ATP-generating system. An in-vitro assay employing cycloheximide, and inhibitor of cytoplasmic protein synthesis, and chloramphenicol, and inhibitor of mitochondrial protein synthesis, to distinguish mitochondrial protein synthesis from the cytoplasmic process. Amino acid incorporation into mitochondria was dependent on the concentration of mitochondria and was inhibited by chloramphenicol. The rate of uptake of tritium leucine into mitochondrial protein was unaffected by the addition of T-2 toxin and was not a rate-limiting step in incorporation. However, 0.02 micrograms/ml of T-2 toxin decreased the rate of protein synthesis inhibition correlated with the amount ...

Science.gov (United States)

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography ...

Energy Technology Data Exchange (ETDEWEB)

This paper presents a unique method, currently under evaluation, that can continuously track pipeline operation providing essentially instantaneous responses to leaks or ruptures without causing a pressure drop or energy loss. Using the highly linear characteristics of a very accurate and repeatable meter and then correlating it to a second identical unit, a dynamic leak detection system is planned that can be retrofitted on existing pipelines without a major construction effort nor destroying the integrity of the existing transmission line. Initial tests performed on a crude oil transmission line verified the equipment's performance and established that the repeatability limits are sufficient to continue development of the concept.

Science.gov (United States)

The fact that South Africa had no commercially significant deposits of crude oil necessitated the early development of production of gasoline from coal which was present in abundance in low to medium grade in thick seams, the development of the process since the early 1950s is discussed. Both the Fischer Tropsch and Lurgi processes are used, and three large plants named Sasol One, Two, and Three have been completed. A flow diagram for the Lurgi process as used in these plants is included. Typical products and their properties resulting from the Sasol-type Synthol operation are presented in tabular form. Marketing considerations and economics of production of the Sasol products are discussed briefly. (BLM)

International Nuclear Information System (INIS)

This paper describes the chemical constituents isolated from aerial parts of the plant Galianthe brasiliensis. From a methanol extract, the iridoid glycosides asperuloside, deacetylasperuloside, mixture of Z- and E-6-O-p-coumaroylscandoside methyl ester, the triterpene ursolic acid and the steroids stigmasterol, campesterol, b-sitosterol and 3-O-b-glucopyranosyl sitosterol were isolated. The structures of the natural products were identified on the basis of spectral data, including 2D NMR experiments. The antiproliferative properties of the crude methanolic extract were investigated against a series of nine human cancer cell lines. (author)

Energy Technology Data Exchange (ETDEWEB)

Floating Production, Offshore Storage and Offloading (FPSO) of hydrocarbons is not new. However, offshore crude oil development has to face the increasing need of the geopolitical interests to develop smaller or difficult reservoir in remote areas in deeper water and harsher environment. Such a trend can only be sustained if the offshore technology provides the right tools at the right time and at an acceptable price. This article will attempt to describe the relative merits of FPSO systems and discuss its main components. An overview of the present world situation shows the excellent performance of the FPSO with the type of development strategy undertaken in P.R.C.

Energy Technology Data Exchange (ETDEWEB)

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

International Nuclear Information System (INIS)

Amoco Trinidad Oil Company produces 60,000 bbl/d of oil from the Trinidadian offshore. The oil is pipelined ashore where it is processed and returned offshore to a buoy mooring for transport up Trinidad's east coast. Amoco Trinidad has developed comprehensive oil spill contingency plans, starting from computer models of spill scenarios. The models used initially assumed that the oils would emulsify quickly and the spills would become highly viscous and persistent, reaching the shoreline in 15-24 h. Such behavior would render ineffective the use of dispersants as a spill countermeasure. Studies showed a poor potential capability of physical recovery systems for spills off the Trinidad east coast due to high sea states, strong winds, and other factors. These results led to questioning of the spill model's assumptions, and laboratory tests were conducted to study the actual behavior of the crude oils. It was found that the oil was difficult to emulsify and highly ...

Energy Technology Data Exchange (ETDEWEB)

In world crude oil markets, 1987 was a year of limited recovery and relative stability after the dramatic price slide of early 1986. Both foreign and domestic crude thereafter, ending the year somewhat higher than a year ago. In contrast, product wholesale markets remained relatively stable throughout the year, while retail prices sustained a fairly steady increase. As has been the case for over a decade, major price movements in international oil markets generally reflected responses to actual or perceived changes in the policies of the Organization of Petroleum Exporting Countries (OPEC) and/or its members. The year began with prices on an upward trend, in reaction to the December 1986 OPEC meeting in which the members resolved to return to an official pricing structure (a departure from the market-based pricing of 1986), and to reduce output quotas. Prices continued to rise until August, when evidence of continued OPEC overproduction ...

Energy Technology Data Exchange (ETDEWEB)

We have recently introduced several important improvements in the measurement of distillation curves for complex fluids. This new method is a significant improvement over current approaches, with features including a composition-explicit data channel for each distillate fraction (for both qualitative and quantitative analysis), trace chemical analysis of each distillate fraction, and a corrosivity assessment of each distillate fraction. In the present contribution, we present the application of the advanced distillation curve method to a bio-derived crude oil prepared from swine manure. One motivation behind the work was to precisely measure the distillation curve of the oil derived from swine manure with our advanced distillation apparatus and compare the bio-derived oil to traditional petroleum crude oils. Then, the information content of the bio-derived oil distillation was extended further with the composition-explicit data channel: gas ...

British Library Electronic Table of Contents (United Kingdom)

Cocoon sericin plays an important role in the reeling of silk and serves as a valuable biomaterial in the field of biomedicine, skincare, and food industries; however, knowledge about cocoon sericin proteins has been limited. For a comprehensive study on sericin, cocoons of eight varieties of silkworm of different geographic origin and with varied cocoon color were analyzed utilizing proteomics and bioinformatics approaches. The electrophoresis pattern demonstrated some common protein bands for all silkworm varieties and distinctive protein bands for some of those examined in the present study. The Ser2 protein, a new Ser3 protein, and four other novel sericin proteins were identified in cocoons for the first time. Products of both Ser1 and Ser3 genes appear to be ubiquitous in the cocoon ...

British Library Electronic Table of Contents (United Kingdom)

SR proteins are essential splicing factors whose function is controlled by multi-site phosphorylation of a C-terminal domain rich in arginine-serine repeats (RS domain). The protein kinase SRPK1 has been shown to polyphosphorylate the N-terminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear entry of this splicing factor and engagement in splicing function. Later, dephosphorylation is required for maturation of the spliceosome and other RNA processing steps. While phosphates are attached to RS1 in a sequential manner by SRPK1, little is known about how they are removed. To investigate factors that control dephosphorylation, we monitored region-specific mapping of phosphorylation sites in ASF/SF2 as a function of the protein phosphatase PP1. W...

International Nuclear Information System (INIS)

A number of procedures in diagnostic radiology and cardiology make use of long exposures to x-rays from fluoroscopy units. Although numerous studies have been performed to measure or calculate skin dose from these procedures, there have only been a handful of studies to determine the dose to the other organs. This thesis was focused on devising a method to calculate the absorbed dose to underlying tissues and organs. The work was performed in several stages. First, a commercial convolution/superposition-based treatment planning system used in radiation oncology was modified and complemented to make it usable with the low energies of x-rays used in diagnostic radiology. This required generation of energy deposition kernels in the kilovoltage energy range. The kernels were generated using the EGS4 Monte Carlo system of codes and added to the treatment planning system. The treatment planning system was then evaluated for its accuracy of ...

International Nuclear Information System (INIS)

In radiotherapy treatment planning, convolution/superposition algorithms currently represent the best practical approach for accurate photon dose calculation in heterogeneous tissues. In this work, the implementation, accuracy and performance of the FFT convolution (FFTC) and multigrid superposition (MGS) algorithms are presented. The FFTC and MGS models use the same 'TERMA' calculation and are commissioned using the same parameters. Both models use the same spectra, incorporate the same off-axis softening and base incident lateral fluence on the same measurements. In addition, corrections are explicitly applied to the polyenergetic and parallel kernel approximations, and electron contamination is modelled. Spectra generated by Monte Carlo (MC) modelling of treatment heads are used. Calculations using the MC spectra were in excellent agreement with measurements for many linear accelerator types. To speed up the calculations, a number of calculation techniques were ...

International Nuclear Information System (INIS)

Using Monte Carlo simulation and the convolution/superposition algorithm, this work examines percent depth dose curves of the central axis in an acrylic phantom (20x20x20 cm"3) with variously sized air cavities (20x20x1.0, 20x20x2.0, 20x20x3.0, 20x20x4.0 and 20x20x4.95 cm"3 for study of longitudinal electron disequilibrium (ED) and 3.6x3.6x4.95, 4.5x4.5x4.95, 5.4x5.4x4.95 and 20x20x4.95 cm"3 for study of lateral ED). Radiochromic film samples are also measured to verify the Monte Carlo results. The Monte Carlo simulation is performed using OMEGA/BEAM and DOSXYZ codes, and the convolution/superposition calculation relies on an ADAC commercial treatment planning system. Underestimating the dose kernel expansion leads to overestimating the dose of what was found in the air cavity of ED using the convolution/superposition algorithm. Consequently, the dose in the rebuild-up region is influenced. The influenced region is on the acrylic phantom surface to a depth of about ...

UK PubMed Central (United Kingdom)

Werner syndrome (WS) is characterized by premature onset of age-associated disorders and predisposition to cancer. The WS protein, WRN, encodes 3′ → 5′ DNA helicase and 3′...Full Text Available

UK PubMed Central (United Kingdom)

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

UK PubMed Central (United Kingdom)

Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase...Full Text Available

UK PubMed Central (United Kingdom)

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

UK PubMed Central (United Kingdom)

Altered structure, and hence function, of cellular macromolecules caused by oxidation can contribute to loss of physiological function with age. Here, we tested whether the lifespan of bats, which generally...Full Text Available

UK PubMed Central (United Kingdom)

Previous studies have shown that salicylates and protein-calorie malnutrition independently compromise maturation and growth of infants. In the present study, pregnant rats were fed normal-and low-protein...Full Text Available

UK PubMed Central (United Kingdom)

Surfactant protein A (SP-A), first identified as a component of the lung surfactant system, is now recognized to be an important contributor to host defence mechanisms. SP-A can facilitate phagocytosis...Full Text Available

UK PubMed Central (United Kingdom)

The e.p.r. spectra of the Fe-proteins of nitrogenase from all sources studied have unusual features in that they have very anisotropic linewidths and low integrated intensities. These characteristics...Full Text Available

UK PubMed Central (United Kingdom)

Brucella abortus is a facultative, intracellular zoonotic pathogen which can cause undulant fever in humans and abortions in cattle. A 14-kDa protein of B. abortus...Full Text Available

UK PubMed Central (United Kingdom)

Carbenoxolone (CBX) is a semisynthetic derivative of the licorice root substance glycyrrhizinic acid and has been previously reported to induce only heat shock protein 70 [Hsp70, HSPA1A (the systematic...Full Text Available

UK PubMed Central (United Kingdom)

Protein post-translational modifications are an important biological regulatory mechanism, and the rate of their discovery using high throughput techniques is rapidly increasingly. To make use of this...Full Text Available

UK PubMed Central (United Kingdom)

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

UK PubMed Central (United Kingdom)

The kinetic parameters of single bonds between neural cell adhesion molecules were determined from atomic force microscope measurements of the forced dissociation of the homophilic protein-protein bonds....Full Text Available

UK PubMed Central (United Kingdom)

Centrioles are intriguing cylindrical organelles composed of triplet microtubules. Proteomic data suggest that a large number of proteins besides tubulin are necessary for the formation and maintenance...Full Text Available

UK PubMed Central (United Kingdom)

Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing...Full Text Available

UK PubMed Central (United Kingdom)

We had previously identified a macrophage surface protein whose expression is highly induced, transient, and specific, as it is restricted to actively fusing macrophages in vitro and in vivo. This protein...Full Text Available

UK PubMed Central (United Kingdom)

An interactive system for computer analysis of nucleic acid and protein sequences has been developed for the Los Alamos DNA Sequence Database. It provides a convenient way to search or verify various...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

Science.gov (United States)

... 2003)050[0600:LBOTMP]2.0.CO;2 Lectin Binding of the Major Polar Tube Protein (PTP1) ... PTP1. We, therefore, undertook a study ...

UK PubMed Central (United Kingdom)

Dysregulation of the alternative pathway of complement activation, caused by mutations or polymorphisms in the genes encoding factor H, membrane co-factor protein, factor I or factor B, is associated...Full Text Available

UK PubMed Central (United Kingdom)

AMP-activated-protein-kinase (AMPK) is a key sensor and regulator of cellular and whole-body energy metabolism and plays a key role in regulation of lipid metabolism. Since lipid metabolism...Full Text Available

UK PubMed Central (United Kingdom)

Gastrin-releasing peptide receptor (GRPR), a member of the G protein-coupled receptor superfamily, has been utilized for receptor-mediated targeting of imaging and therapeutic agents; here we extend...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

In isolated rat adipocytes, insulin inhibits lipolysis to a greater extent than would be predicted by the decrease in (-/+)cAMP activity ratio of cAMP-dependent protein kinase (A-kinase), from which it was speculated that insulin promotes the dephosphorylation of hormone-sensitive lipase. They have examined the phosphorylation state of cellular proteins under conditions of varying A-kinase activities in the presence and absence of insulin. Protein phosphorylation was determined by SDS-PAGE electrophoresis of extracts from /sup 32/P-loaded cells; glycerol and A-kinase activity ratios were measured in the cytosolic extracts from control, non-radioactive cells. Increased protein phosphorylation in general occurred over the same range of A-kinase activity ratios, 0.1-0.3, associated with increased glycerol release. The insulin-induced decrease in lipolysis was associated with a decrease in the /sup 32/P ...

UK PubMed Central (United Kingdom)

One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions. We present...Full Text Available

UK PubMed Central (United Kingdom)

The levels of in vitro protein binding of cefonicid and cefuroxime in human adult and neonatal sera were compared. Binding parameters for each drug were determined within the concentration range of...Full Text Available

UK PubMed Central (United Kingdom)

Numerous studies have noted that the evolution of new enzymatic specificities is accompanied by loss of the protein's thermodynamic stability (ΔΔG), thus suggesting...Full Text Available

UK PubMed Central (United Kingdom)

Parasitic nematodes cause serious diseases in humans, animals, and plants. They have limited lipid metabolism and are reliant on lipid-binding proteins to acquire these metabolites from their hosts....Full Text Available

Energy Technology Data Exchange (ETDEWEB)

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis ...

UK PubMed Central (United Kingdom)

Mitogen Activated Protein Kinases (MAPKs) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and...Full Text Available

UK PubMed Central (United Kingdom)

A commercial blend of essential oil (EO) compounds was added to a grass, maize silage, and concentrate diet fed to dairy cattle in order to determine their influence on protein metabolism by ruminal...Full Text Available

UK PubMed Central (United Kingdom)

The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater...Full Text Available

UK PubMed Central (United Kingdom)

1 The plasma protein binding of theophylline was determined after addition of [14C]-theophylline (15 micrograms/ml) to plasma from 24 healthy drug-free volunteers and equilibrium dialysis for 2 h at...Full Text Available

UK PubMed Central (United Kingdom)

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...Full Text Available

UK PubMed Central (United Kingdom)

Reversible protein glutathionylation plays a key role in cellular regulation and cell signaling and protects protein thiols from hyperoxidation. Sulfiredoxin (Srx), an enzyme that catalyzes the reduction...Full Text Available

UK PubMed Central (United Kingdom)

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

UK PubMed Central (United Kingdom)

AbstractX-ray diffraction analysis of pressure-induced structural changes in the Aequorea yellow fluorescent protein Citrine reveals the structural basis for the continuous...Full Text Available

UK PubMed Central (United Kingdom)

AbstractThe present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundOne virulence property of Borrelia burgdorferi is its resistance to innate immunity, in particular to complement-mediated killing. Serum-resistant B....Full Text Available

UK PubMed Central (United Kingdom)

Cellular cholesterol homeostasis is controlled by sterol-regulated proteolysis of membrane-bound transcription factors called sterol-regulatory element binding proteins (SREBPs). CPP32, a cysteine protease,...Full Text Available

UK PubMed Central (United Kingdom)

The aim of the present study was to describe the physicochemical characteristics of streptococcal T antigen. T protein isolated from Streptococcus pyogenes type 12 (R53/1077, Colindale) and purified...Full Text Available

UK PubMed Central (United Kingdom)

Recently, CID755673 was reported to act as a highly selective inhibitor of protein kinase D (PKD). In the course of experiments using CID755673, we noticed that it exerted unexpected stimulatory...Full Text Available

UK PubMed Central (United Kingdom)

Certain pathogenic species of Bacillus and Clostridium have developed unique methods for intoxicating cells that employ the classic enzymatic “A-B” paradigm for protein toxins. The binary...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundMolecular docking methods are commonly used for predicting binding modes and energies of ligands to proteins. For accurate complex geometry and binding energy estimation,...Full Text Available

UK PubMed Central (United Kingdom)

The use of sub-nanometer resolution electron density as spatial constraints for denovo and ab-initio structure prediction requires knowledge of protein boundaries...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

UK PubMed Central (United Kingdom)

To study the effect of agonist on the TRH (thyrotrophin-releasing hormone) receptor protein, an epitope-tagged receptor was stably expressed in HEK-293 cells (human embryonic kidney 293 cells) and receptor...Full Text Available

UK PubMed Central (United Kingdom)

Ataxin-3, the protein involved in Machado-Joseph disease, is able to bind ubiquitylated substrates and act as a deubiquitylating enzyme in vitro, and it has been involved in the modulation of protein...Full Text Available

UK PubMed Central (United Kingdom)

AMP-activated protein kinase (AMPK) was initially viewed as energy sensor and activated by increased intracellular concentrations of AMP following nutrient deprivation. Physiological or pathological...Full Text Available

UK PubMed Central (United Kingdom)

SummaryThe prevalence of paralogous enzymes implies that novel catalytic functions can evolve on preexisting protein scaffolds. The weak secondary activities of proteins, which...Full Text Available

International Nuclear Information System (INIS)

As part of efforts to develop improved methods for NMR protein sample preparation and structure determination, the Northeast Structural Genomics Consortium (NESG) has implemented an NMR screening pipeline for protein target selection, construct optimization, and buffer optimization, incorporating efficient microscale NMR screening of proteins using a micro-cryoprobe. The process is feasible because the newest generation probe requires only small amounts of protein, typically 30-200 ?g in 8-35 ?l volume. Extensive automation has been made possible by the combination of database tools, mechanization of key process steps, and the use of a micro-cryoprobe that gives excellent data while requiring little optimization and manual setup. In this perspective, we describe the overall process used by the NESG for screening NMR samples as part of a sample optimization process, assessing optimal construct design and ...

UK PubMed Central (United Kingdom)

BackgroundProteins may evolve through the recruitment and modification of discrete domains, and in many cases, protein action can be dissected at the domain level. PDZ domains are...Full Text Available

UK PubMed Central (United Kingdom)

In addition to inducing new transcriptional activities that lead within a few hours to the accumulation of heat shock proteins (Hsps), heat shock activates within minutes the major signaling transduction...Full Text Available

KoreaScience (Korea)

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KoreaScience (Korea)

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International Nuclear Information System (INIS)

... carbon 14 cholera gels labelling membranes molecules proteins receptors

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

Science.gov (United States)

... Considering these observations, it is most likely that Brucela proteins involved in protective immunity will preferentially stimulate INF-g producing T ...

KoreaScience (Korea)

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International Nuclear Information System (INIS)

The effect of protein malnutrition on the metabolism of collagen in bone was studied in young female albino rats after a single injection of "3H-proline. Both specific and total radioactivities of hydroxyproline in the total collagen of the bone were found to decrease in the protein-deficient animals, indicating decreased rate of collagen synthesis. In the urine the amount of hydroxyproline excreted and total radioactivity of "3H-hydroxyproline were greatly decreased. The results of the present investigation therefore clearly indicate decreased synthesis and catabolism of collagen in bones of protein deficient animals compared to controls. (auth.).

International Nuclear Information System (INIS)

Endurance-trained older men have a higher proportion of lean tissue and greater muscle cell oxidative capacity, reversing age-related trends and suggesting major changes in protein metabolism. In this study, protein turnover was determined in 6 middle-aged (52+/-1 yr) men who were well trained (VO_2 max 55.2+/-5.0 ml O_2/kg.min) and lean (body fat 18.9+/-2.8%, muscle mass 36.6+/-0.6%). The maintained habitual exercise while consuming 0.6, 0.9 or 1.2 g protein/kg.day for 10-day periods. N flux was measured from "1"5N in urea after oral "1"5N-glycine administration. Myofibrillar protein breakdown was estimated from urinary 3-methyl-histidine. Dietary protein had no effect on turnover rates, even when N balance was negative. Whole body protein synthesis was 3.60+/-0.12 g/kg.day and breakdown was 3.40+/-0.14 g/kg.day for all N intakes. Whole body ...

UK PubMed Central (United Kingdom)

In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal...Full Text Available

Science.gov (United States)

This study investigated relationships between molecular weight distributions of unreduced grain proteins and grain, flour, and end-use quality characteristics of soft white winter wheats grown in Oregon. Absorbance area and area % values of protein fractions separated by size exclusion HPLC (SE-HPL...

UK PubMed Central (United Kingdom)

The discovery of a specific high-affinity growth hormone (GH) binding protein (GH-BP) in plasma adds complexity to the dynamics of GH secretion and clearance. Intuitive predictions are that such a protein...Full Text Available

UK PubMed Central (United Kingdom)

Gamma interferon plays an important role in regulating the functional properties of mononuclear phagocytes. In the present study, the role of activated protein kinases in the mechanism of action of...Full Text Available

UK PubMed Central (United Kingdom)

The major core protein (p28) of MMC-1, an endogenous type C virus of the rhesus monkey (Macaca mulatta), was purified and subjected to structural and immunological analyses. The NH2-terminal amino acid...Full Text Available

International Nuclear Information System (INIS)

It is a useful hypothesis to thing of the reaction to protein deprivation as made up of two parts. First there is an initial adaptation involving rapid losses of protein. This is followed by slow continuing depletion. When considering two organ groups, such as the liver and muscle, it appears that the curve of urinary nitrogen excretion reflects the different parts they play in these two phases. The changes in the relative amounts of protein in different organs results in an alteration in the pattern of body protein at the level of the organ, the tissue and the cell. These changes are themselves the result of altered rates of synthesis and breakdown. The questions that must be asked and hopefully will be answered with the help of isotopes, are how these changes are brought about, how they are inter-related and controlled, and what is their significance? This paper contains a discussion of the few ...

British Library Electronic Table of Contents (United Kingdom)

Frozen boiled shrimp and dried shrimp are among the high-value fishery products of Thailand. During the production of these products boiling is one of the most important steps that affects significantly the product physicochemical properties, especially the quantity and quality of proteins, which in turn affect other apparent properties perceived by consumers. The protein changes are, however, difficult to evaluate comparing to other typical physical properties of shrimp. The objective of this study was therefore to develop an artificial neural network (ANN) model to predict the protein changes of shrimp in terms of protein loss and protein denaturation as a function of the boiling conditions, namely, concentration of salt solution and boiling time, as well as a rather easily determined ch...

Energy Technology Data Exchange (ETDEWEB)

The effect of experimental diabetes on the phosphorylation of proteins in the rat sciatic nerve was studied. Nerves from animals made diabetic with streptozocin were incubated in vitro with (/sup 32/P)orthophosphate and divided into segments from the proximal to the distal end, and proteins from each segment were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The principal labeled species were the major myelin proteins, P0, and the basic proteins. After 6 wk of diabetes, the incorporation of isotope into these proteins rose as a function of distance along the nerve in a proximal to distal direction and was significantly higher at the distal end compared with incorporation into nerves from age-matched controls. The overall level of isotope uptake was similar in nerves from diabetic animals and weight-matched controls. The distribution of /sup 32/P among ...

Energy Technology Data Exchange (ETDEWEB)

Light increased the rate of LHCP synthesis as measured by pulse-labeling with /sup 35/SO/sub 4/ and immunoprecipitation with antibody specific for Euglena LHCP. In addition to the mature LHCP, 26,000 daltons, the LHCP specific antibody immunoprecipitated large amounts of several proteins having molecular weights of approximately 100,000. On immunoblots of immunoprecipitated unlabeled protein, the antibody only detected the mature LHCP suggesting that the high molecular weight proteins are not LHCP aggregates produced during immunoprecipitation. After a 10 min pulse with /sup 35/SO/sub 4/, the 100,000 dalton proteins constituted over 80% of the immunoprecipitated material. In a subsequent chase, the radioactivity in the 100,000 dalton proteins decreased and the radioactivity in the mature LHCP increased suggesting a precursor-product relationship. After a 35 minute chase, the mature ...

International Nuclear Information System (INIS)

Turkey's natural gas (NG) production is very small and is almost all imported. Turkish natural gas production in 2000, 23 billion cubic feet (Bcf), met around 4% of domestic natural gas consumption requirements. NG consumption is estimated at around 700 Bcf in year 2002, accounting for around 17% of Turkey's total energy consumption. Turkish natural gas demand had been projected to increase extremely rapidly in coming years. Country oil production is 50,674 barrels per day (bbl/d) of which 46,674 bbl/d was crude oil in 2002. In 2002, oil consumption was 635,000 bbl/d and net oil imports was 584,326 bbl/d in Turkey. Oil provides around 43% of Turkey's total energy requirements. (Author)

British Library Electronic Table of Contents (United Kingdom)

The analysis of complex fluids such as crude oils, fuels, vegetable oils and mixed waste streams poses significant challenges arising primarily from the multiplicity of components, the different properties of the components (polarity, polarizability, etc.) and matrix properties. We have recently introduced an analytical strategy that simplifies many of these analyses, and provides the added potential of linking compositional information with physical property information. This aspect can be used to facilitate equation of state development for the complex fluids. In addition to chemical characterization, the approach provides the ability to calculate thermodynamic properties for such complex heterogeneous streams. The technique is based on the advanced distillation curve (ADC) metrology, wh...

Energy Technology Data Exchange (ETDEWEB)

In the world of pipeline maintenance, there are pigs and there are intelligent pigs. Intelligent pigs, when launched into a pipeline, carry instrumentation onboard which enables them to gather and record information about problems such as denting, corrosion, and cracking. More basic pigs, on the other hand, look after the routine internal maintenance chores such as cleaning and swabbing, or else perform operational duties such as batching different types of product along the line. The harsh environment in which pigs often operate is described. Aside from the mechanical shocks they undergo when passing welds, bends, and other fittings, they may have to contend with hostile conditions such as hydrogen sulfide environments. High pressure is another factor to consider. In crude oil lines, significant paraffin deposits also can cause problems if they affect the sensors ability to maintain good contact with the pipe wall. In spite of these considerations, increasing ...

Energy Technology Data Exchange (ETDEWEB)

The removal phenol and 2-chlorophenol using soybean seed-hulls in the presence of hydrogen peroxide was demonstrated. The performance of a stirred membrane reactor containing soluble purified SEP was compared with a batch stirred reactor containing raw soybean seed-hulls. The purified enzyme reactor proved to be ineffective while good results were obtained with the crude seed-hulls for the removal of phenol and 2-chlorophenol. A single batch reactor containing raw seed-hulls was effective in removing more than 98.5% of 2-chlorophenol (initially at 10000 ppm) in less than 15 min. The performance of these rectors is comparable to existing HRP-based technology. The stability of the soybean peroxidase (SBP) enzyme was also examined in the presence of detergents (SDS, Tween 20 and Triton X-100).