UK PubMed Central (United Kingdom)

Brassica napus is an allotetraploid (AACC) formed from the fusion of two diploid progenitors, Brassica rapa (AA) and Brassica oleracea...Full Text Available

UK PubMed Central (United Kingdom)

Digestion of plant chromatin from Brassica pekinensis and Matthiola incana with staphylococcus nuclease leads to a DNA repeat of 175 plus or minus 8 and a core size of 140 base pairs. DNase I digestion...Full Text Available

UK PubMed Central (United Kingdom)

Isolated cauliflower (Brassica oleracea) bud plastids, purified by isopycnic centrifugation in density gradients of Percoll, were found to be highly intact, to be practically devoid...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzy...

UK PubMed Central (United Kingdom)

The endogenous levels of several plant growth substances (indole acetic acid, IAA; abscisic acid, ABA; zeatin, Z; zeatin riboside, [9R]Z; isopentenyladenine, iP; and isopentenyladenosine, [9R]iP were...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Abstract Turnip (Brassica rapa L.) plants were grown at adequate (25 mmol L-1) or low (<2.5 mmol L-1) boron (B) supply in nutrient solution for 1 month. The shoot and root dry weight was inhibited by up to 77% and 45%, respectively, in response to low B supply. The results of a retranslocation experiment showed that loaded B in the mature leaves was depleted rapidly during the experimental period and that this B was retranslocated to younger leaves as judged by B depletion from mature leaves simultaneously with the appearance of B in new leaves. Up to 89% of the B content of mature leaves was lost during 4 weeks of growth under B-deficient conditions. In addition, in B-deficient plants, a greater proportion of the total plant B was allocated to young leaves compared with B-sufficient plant...

British Library Electronic Table of Contents (United Kingdom)

2,6-Diacetyl pyridine based ligand was synthesized by the reaction of 2,6-diacetyl pyridine with thiocarbohydrazide in presence of acetic acid. The coordination compounds with Cr(III) and Ni(II) metal ions having [Cr(L)X]X2 and [Ni(L)X]X compositions (where L=ligand and X=NO3^-, Cl^- and CH3COO^-) were synthesized and characterized by physicochemical and spectral studies. The studies like elemental analyses, molar conductance measurements, magnetic susceptibility measurements, IR, UV-Vis, NMR, mass and EPR reveal that the complexes are octahedral. The compounds were examined against the pathogenic fungal and bacterial strains like Alternaria brassicae, Aspergillus niger, Fusarium oxysporum, Xanthomonas compestris and Pseudomonas aeruginosa. A. niger causes the diseases Apergillosis and Oto...

International Nuclear Information System (INIS)

Sodium is one of the most essential elements needed for metabolic processes amongst human beings. It is consumed in the form of sodium chloride but it is also present in edible plant leaves. Sodium is mostly analyzed by flame photometric method, a destructive and time consuming technique. Sodium has been determined in some green leave vegetables samples-palak, radish, khatta palak (ambat chuka), chaulai leaves, chauli bean covers and its seeds by instrumental neutron activation analysis. The method involves irradiation of samples with thermal neutrons from "2"4"1Am-Be source and counting "2"4Na activity (half life 15 hr) from the reaction "2"3Na(n,#gamma#) "2"4Na. Activity due to 1.37 MeV photopeak was counted with a NaI(Tl) crystal coupled to gamma ray spectrometer. Green leaves of the vegetables were thoroughly washed, dried at constant temperature and powdered. Bowen's Kale powder was used as standard for measuring sodium abundances. About 2g each of samples and ...

International Nuclear Information System (INIS)

Application of inorganic S upto 20 mg kg"-"1 soil increased the grain yield of toria and total uptake of S. Incorporation of green manure (above ground portion) alongwith inorganic S further increased the grain yield of toria as well as sulphur uptake. Maximum grain yield of toria was obtained when green manure was supplemented with 10 mg S kg"-"1 soil. Cowpea as green manure proved superior to guara. Per cent S derived from green manures (SdfGM) decreased with increase in the level of inorganic S and ranged from 12.3 to 25.1. Total amount of S supplied by cowpea and guara in presence of varying levels of inorganic S ranged from 7.6 to 10.5 and 5.9 to 8.6 mg pot"-"1, respectively. Correspondingly, the per cent utilization of S ranged from 24.5 to 33.9 and 19.4 to 28.7, respectively. The results suggest that S from green manures significantly contributed to S nutrition of oilseed crops and it should be considered for better management of S. (author). 8 refs., 4 tabs.

Energy Technology Data Exchange (ETDEWEB)

Metal nanoparticles have many potential technological applications. Biological routes to the synthesis of these particles have been proposed including production by vascular plants, known as phytoextraction. While many studies have looked at metal uptake by plants, particularly with regard to phytoremediation and hyperaccumulation, few have distinguished between metal deposition and metal salt accumulation. This work describes the uptake of AgNO{sub 3}, Na{sub 3}Ag(S{sub 2}O{sub 3}){sub 2}, and Ag(NH{sub 3}){sub 2}NO{sub 3} solutions by hydroponically grown Brassica juncea and the quantitative measurement of the conversion of these salts to silver metal nanoparticles. Using X-ray absorption near edge spectroscopy (XANES) to determine the metal speciation within the plants, combined with atomic absorption spectroscopy (AAS) for total Ag, the quantity of reduction of Ag{sup I} to Ag{sup 0} is reported. Transmission electron microscopy (TEM) showed Ag particles of ...

Energy Technology Data Exchange (ETDEWEB)

Most prior climate change assessments for U.S. agriculture have focused on major world food crops such as wheat and maize. While useful from a national and global perspective, these results are not particularly relevant to the Northeastern U.S. agriculture economy, which is dominated by dairy milk production, and high-value horticultural crops such as apples (Malus domestica), grapes (vitis vinifera), sweet corn (Zea mays var. rugosa), cabbage (Brassica oleracea var. capitata), and maple syrup (sugar maple, Acer saccharum). We used statistically downscaled climate projections generated by the HadCM3 atmosphere-ocean general circulation model, run with Intergovernmental Panel on Climate Change future emissions scenarios Alfi (higher) and B1 (lower), to evaluate several climate thresholds of direct relevance to agriculture in the region. A longer (frostfree) growing season could create new opportunities for farmers with enough capital to take risks on new crops ...