Science.gov (United States)

We examined the subcellular localization of ryanodine receptors (RyR) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods and compared it with those of rats and guinea pigs. To achieve this goal, an anti-RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs. Western blot analysis using this antibody detected a single RyR band following the SDS-PAGE of sarcoplasmic reticulum (SR) membranes from carp atrium and ventricle as well as from mammalian hearts and skeletal muscles. The carp heart band had slightly greater mobility than those of mammalian hearts. Although immunohistochemical staining showed evident striations corresponding to the Z lines in longitudinal sections of mammalian hearts, clusters of punctate staining, in contrast, were distributed ubiquitously throughout ...

British Library Electronic Table of Contents (United Kingdom)

T-2 toxin, a highly toxic member of the type-A trichothecenes, is produced by various Fusarium moulds that can potentially affect human health. It is strongly cytotoxic for human hematopoietic progenitors. Alimentary toxic aleukia (ATA), a disease typically associated with human, is primarily induced by T-2 toxin. A comparison of the metabolism of T-2 toxin incubated with hepatocytes of rats, piglets, chickens, and the hepatic subcellular fractions (microsomes and cytosol) of piglets, chickens, rats, and carp (common carp and grass carp) was carried out. The activities of the recombinant pig CYP3A29 on the transformation of T-2 and HT-2 toxins were preliminary studied. Metabolites were identified by novel LC/MS-IT-TOF. Qualitative similarities and differences across the species were observ...

UK PubMed Central (United Kingdom)

The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available

CERN Document Server

This paper is about two arrangements of hyperplanes. The first --- the Shi arrangement --- was introduced by Jian-Yi Shi to describe the Kazhdan-Lusztig cells in the affine Weyl group of type $A$. The second --- the Ish arrangement --- was recently defined by the first author who used the two arrangements together to give a new interpretation of the $q,t$-Catalan numbers of Garsia and Haiman. In the present paper we will define a mysterious "combinatorial symmetry" between the two arrangements and show that this symmetry preserves a great deal of information. For example, the Shi and Ish arrangements share the same characteristic polynomial, the same numbers of regions, bounded regions, dominant regions, regions with $c$ "ceilings" and $d$ "degrees of freedom", etc. Moreover, all of these results hold in the greater generality of "deleted" Shi and Ish arrangements corresponding to an arbitrary subgraph of the complete graph. Our proofs are based on nice ...