ige antibody production: Topics by WorldWideScience.org

A new approach to the isolation and characterization of wheat flour allergens

An aqueous house dust mite extract was separated by SDS-PAGE and transferred to nitrocellulose membranes by Western Blotting. Two major allergens, Der pI and Der pII associated with the mite faeces...Full Text Available

1988-06-01

3

British Library Electronic Table of Contents (United Kingdom)

Summary Background The incidence of food allergy to wheat is increasing. Its diagnosis depends on the purity of major allergens and their inclusion in tests. Isolation and characterization of wheat allergens are therefore of utmost importance. Objective To purify and identify wheat flour allergens most frequently recognized by patients' IgE antibodies and to study their allergenicity. Methods Water/salt-soluble extracts from wheat flour were prepared and separated using a combination of ultrafiltration, isoelectric focusing and liquid chromatography. Purified proteins were analysed by immunoblotting using pooled sera from patients with atopic dermatitis who possessed IgE specific to wheat. Wheat proteins found to bind IgE were subsequently identified by matrix-assisted laser desorption/ion...

2011-01-01

4

Protective efficiency of dendrosomes as novel nano-sized adjuvants for DNA vaccination against birch pollen allergy

British Library Electronic Table of Contents (United Kingdom)

We evaluated the use of a novel gene porter (Den123-a nontoxic self-assembled dendritic spheroidal nanoparticle made of biodegradable monomers), aiming to enhance and improve the desired immune response in protection from allergy. Footpad DNA immunization in Balb/c mice was done three times using the Bet v 1a gene with or without Den123 with 2-week intervals followed by sensitization with rBetv1 (5mg) in alum twice in a weekly interval. Different doses of pCMV-Betv1 were used (10mg and 100mg). The protective role of different formulations was evaluated by measuring the IgG1, IgG2a and IgE antibody production, cytokine release of isolated splenocytes and b-hexosaminidase release from the RBL cells. Higher and increasing ratios of IgG2a/IgG1 were seen in mice which received plasmids in combi...

2006-01-01

5

Homocytotropic antibodies (IgE) to Neisseria gonorrhoeae in the rat and a cross-reactivity of heterologous gonococcal strains.

Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans.

Outbred Wistar rats were immunised with a single intraperitoneal injection of a mixture of 30 mg of A1(OH)3 and 100microng of gonococcal zeolite antigen (ZA). Ten days after immunisation, ZA prepared...Full Text Available

1977-04-01

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A comprehensive comparison of mixing, mass transfer, Chinese hamster ovary cell growth, and antibody production using Rushton turbine and marine impellers

Udgivelsesdato: 1985-Feb

1985-01-01

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British Library Electronic Table of Contents (United Kingdom)

Large scale production of monoclonal antibodies has been accomplished using bioreactors with different length to diameter ratios, and diverse impeller and sparger designs. The differences in these physical attributes often result in dissimilar mass transfer, mechanical stresses due to turbulence and mixing inside the bioreactor that may lead to disparities in cell growth and antibody production. A rational analysis of impeller design parameters on cell growth, protein expression levels and subsequent antibody production is needed to understand such differences. The purpose of this study was to examine the impact of Rushton turbine and marine impeller designs on Chinese hamster ovary (CHO) cell growth and metabolism, and antibody production and quality. Experiments to evaluate mass transfer...

2011-01-01

8

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

British Library Electronic Table of Contents (United Kingdom)

Abstract The glycosylation profile of therapeutic antibodies is routinely analyzed throughout development to monitor the impact of process parameters and to ensure consistency, efficacy, and safety for clinical and commercial batches of therapeutic products. In this study, unusually high levels of the mannose-5 (Man5) glycoform were observed during the early development of a therapeutic antibody produced from a Chinese hamster ovary (CHO) cell line, model cell line A. Follow up studies indicated that the antibody Man5 level was increased throughout the course of cell culture production as a result of increasing cell culture medium osmolality levels and extending culture duration. With model cell line A, Man5 glycosylation increased more than twofold from 12% to 28% in the fed-batch process...

2011-01-01

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Monoclonal antibodies to antigens on human neutrophils, activated T lymphocytes, and acute leukemia blast cells

A convenient and sensitive allergy test: IgE crosslinking-induced luciferase expression in cultured mast cells

The authors describe the production of two mouse hybridomas secreting monoclonal antibodies to antigenic determinants of the surface membranes of human neutrophils, activated T lymphocytes, and acute leukemic blast cells. The degree of lymphocyte stimulation was estimated from incorporation of /sup 3/H-thymidine with parallel microculture. Monoclonal antibodies of supernatants of hybridoma cultures shown here reacted in both immunofluorescence test and cytotoxicity test with surface membrane antigens on the majority of neutrophils and PHA-activated peripheral blood lymphocytes from healthy subjects, but did not give positive reactions with unactivated lymphocytes, adherent monocytes, erythrocytes, and alloantigen-stimulated lymphocytes.

1987-11-01

10

Radiochemistry of lead-203 for radiolabelling antibody conjugates

BackgroundFor the detection of allergen-specific IgE in sera, solid-phase IgE-binding assays like the CAP test are commonly used. Although such immunochemical methods are very sensitive,...Full Text Available

2010-10-01

11

Production and characterization of monoclonal antibodies recognizing the alpha-chain subunits of human ia alloantigens.

Lead-203 [52.1 h, 279 (80.1%) KeV] has been recognized as a potentially useful tracer for tumor specific radiopharmaceuticals due to its favorable nuclear and chemical properties. This paper reports the cyclotron production of lead-203 and the labelling of monoclonal antibody B72.3, conjugated with 2-(p-isothiocyanatobenzyl)DOTA, with lead-203 in 30% yield. In vivo biodistribution and stability studies in mice are being conducted.

1990-06-24

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Characterization of DTPA complexes and conjugated antibodies of astatine

Two monoclonal antibodies, TAL-1B5 and TAL-3C3, specific for human Ia alpha-chain subunits have been produced by fusing P3/NSI/1-Ag4-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized...Full Text Available

1983-12-01

13

Human and rat mast cell high-affinity immunoglobulin E receptors: Characterization of putative. alpha. -chain gene products

The complex formation of astatine(I) cation with diethylene triamine pentaacetic acid (DTPA) and characterization of the complexes were investigated by electromigration in free electrolytes and by gel-chromatography on Sephadex G 25. The conjugation procedure for the production of At-DTPA conjugated polyclonal antibodies is described. (author) 15 refs.; 2 figs.

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Experimental parameters differentially affect the humoral response of the cholera-toxin-based murine model of food allergy

The authors have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative {alpha} subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH{sub 2}-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hydrophobic transmembrane region, and a positively charged cytoplasmic tail. The human and rat {alpha} subunits share an overall homology with one another and the immunoglobulin gene family, suggesting that they arose from a common ancestral gene and continue to share structural homology with their ligands. In addition, the rat gene is transcribed into at least three distinct forms, each of which yields a somewhat different coding sequence.

1988-03-01

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Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells

Background: Recent studies have developed a murine model of IgE-mediated food allergy based on oral coadministration of antigen and cholera toxin (CT) to establish a maximal response for studying immunopathogenic mechanisms and immunotherapeutic strategies. However, for studying subtle immunomodulating factors or factors effective during response initiation, this maximal response-based model is less suitable due to a lack of sensitivity. Therefore, in attempts to identify essential parameters to fine-tune the immune response towards a submaximal level, potentially more sensitive, we were interested in characterizing the individual effects of the parameters in the CT-based model: CT dose, antigen type and dose, and number of immunizations. Methods: BALB/c mice were orally sensitized weekly for 3 or 7 weeks with graded doses of CT and various food antigens (soy-trypsin inhibitor, ovalbumin or ovomucoid). Antigen-specific lgG1, IgG2a, IgA and IgE were monitored by ...

2003-01-01

16

British Library Electronic Table of Contents (United Kingdom)

In mammals, primordial germ cells (PGCs) are generated in the extra-embryonic epiblast, and thereafter migrate into the developing gonads. Following the development of the gonads to the testes or ovaries, germ cells mature into sperms or eggs. In the present study, we report production and characterization of monoclonal antibodies (MAb) that recognize PGCs. Extracts from E12.5 mouse embryonic gonads were immunized as an antigen, and hybridomas were generated using the rat medial iliac lymph node method. The hybridoma supernatants were screened by immunohistochemical analyses of E12.5 mouse embryonic sections. The antibody, referred to herein as MAb 5B5, provided strong signals on PGCs. Moreover, immunofluorescence analyses using a variety of the tissue sections of mouse embryos revealed th...

2010-01-01

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Immunological detection and quantification of DNA components structurally modified by alkylating carcinogens, mutagens and chemotherapeutic agents

A study of the incubation times of a new solid phase system for the assay of specific IgE with RAST

The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 10"9 to > 10"1"0 lmol) specifically directed against several DNA alkylation ...

1983-09-01

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IgE, Allergy, and Risk of Glioma: Update from the San Francisco Bay Area Adult Glioma Study in the Temozolomide Era

(1985). Italy Pisati, P. Aprile, C. Fondazione Clinica del Lavoro, Pavia (Italy).

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Human rib bone marrow mononuclear cells spontaneously synthesize and secrete IgE in vitro.

The consistently observed inverse relationship of allergic conditions with glioma risk and our previous demonstration that IgE levels also were lower in glioma patients than controls suggest...Full Text Available

2009-08-01

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Euglena light-harvesting chlorophyll A/B binding protein (LHCP) synthesized as an unusually large precursor

We have examined spontaneous secretion of IgE by human rib bone marrow mononuclear cells (MNC). Bone marrow MNC from nine out of 12 rib specimens synthesized and secreted substantial amounts of IgE...Full Text Available

1991-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

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An immunochemical approach to the study of DNA damage and repair. Technical progress report, May 1, 1989--April 30, 1992

Light increased the rate of LHCP synthesis as measured by pulse-labeling with /sup 35/SO/sub 4/ and immunoprecipitation with antibody specific for Euglena LHCP. In addition to the mature LHCP, 26,000 daltons, the LHCP specific antibody immunoprecipitated large amounts of several proteins having molecular weights of approximately 100,000. On immunoblots of immunoprecipitated unlabeled protein, the antibody only detected the mature LHCP suggesting that the high molecular weight proteins are not LHCP aggregates produced during immunoprecipitation. After a 10 min pulse with /sup 35/SO/sub 4/, the 100,000 dalton proteins constituted over 80% of the immunoprecipitated material. In a subsequent chase, the radioactivity in the 100,000 dalton proteins decreased and the radioactivity in the mature LHCP increased suggesting a precursor-product relationship. After a 35 minute chase, the mature LHCP was the major ...

1987-04-01

22

An immunochemical approach to the study of DNA damage and repair

The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We have been studying damages that are stable radiolysis products found in X-irradiated DNA and thus of potential biological consequence. Our aim is to build an in vitro and in vivo data base on the interactions between model DNA lesions and such cellular enzymes as DNA polymerases and repair endonucleases. Initial studies have focused on ...

1992-05-01

23

Validation of immune function testing during a 4-week oral toxicity study with FK506.

The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the conjugates as immunogens in rabbits or to prepare monoclonal antibodies. We have been studying damages that are stable radiolysis products found in X-irradiated DNA and thus of potential biological consequence. Our aim is to build an in vitro and in vivo data base on the interactions between model DNA lesions and such cellular enzymes as DNA polymerases and repair endonucleases. Initial studies have focused on ...

1992-05-01

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Principles of antibody therapy.

Assessment of the immune system's capability to respond to antigens with the generation of specific antibodies, whilst under the influence of a test article, is required in toxicity tests according to the European guideline for repeated dose toxicity testing of medicinal products. The purpose of this study in rats was to validate methodology for the determination of Keyhole Limpet Haemocyanin (KLH)-specific antibodies under the influence of an immunologically active compound. The immunosuppressant FK506, commercially available as Prograf, was administered orally (gavage) to five rats per sex per group at dosages of 0.5mg/kg per day or 3mg/kg per day, for a period of 4 weeks. On days 14 and 22, KLH was administered subcutaneously, with an adjuvant (AluGel), to the two treated groups and a control (i.e. without FK506 treatment) approximately 1h following administration of FK506. Terminal investigations included haematology ...

2004-04-01

25

Studies on metabolism of directly labeled {sup 99}MTc-antibody in mice

The success of monoclonal antibodies in clinical practice is dependent on good design. Finding a suitable target is the most important part as other properties of the antibody can be altered by genetic...Full Text Available

1992-12-05

26

Radiochemistry of lead-203 for radiolabeling antibody conjugates

The elucidation on the metabolic products of the {sup 99}mTc-antibody conjugates may provide insights and approaches that would reduce the undesirable deposition of radioactive species in normal tissue. In this investigation, the radiolabeled species in blood, urine, bile and extracts of liver and kidney obtained at different times after the injection of a model antibody, {sup 99}mTc, into mice were analyzed with various chromatographic methods. Ninety-nine to 100% of the radioactivity in serum was associated with intact Mab 170. The radioactivity in liver homogenate extract was strictly protein-bound to either intact Mab or low molecular weight species (LMW). In kidney extracts, the majority of the radioactivity was protein bound {sup 99}mTc, with less than 8% of the activity being non- protein bound . Multiple {sup 99}mTc -containing protein and non-protein species were found in urine and bile. Evidence supporting the ...

1996-12-01

27

Radiochemistry of lead-203 for radiolabeling antibody conjugates

Short note.

1991-01-01

28

Immunoglobulins in myasthenia gravis. Kinetic properties of the acetylcholine-receptor antibody studied during lymph drainage.

Brief item.

29

Development of sup 203 Pb as a new label for monoclonal antibodies

A specific immunoglobulin, the receptor antibody, can be found in most patients with myasthenia gravis. In order to study the kinetic properties of this antibody, serial determinations of receptor antibody,...Full Text Available

1978-10-01

30

Localization of an anti-tumour monoclonal antibody in human tumour xenografts: kinetic and quantitative studies with the 791T/36 antibody

Brief item.

1989-01-01

31

Radiolabelling of. cap alpha. -bungarotoxin to high specific radioactivity with complete retention of biological activity

Studies have been performed with an anti-osteogenic sarcoma monoclonal antibody 791T/36 and human tumour xenografts to examine some of the quantitative and kinetic aspects of tumour localization. The influence of variations in tumour size and site and antibody dose on the extent and rate of specific antibody localization have been examined, together with the site of intratumoural deposition of the antibody. (UK).

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A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies

Radioiodinated ..cap alpha..-bungarotoxin, the venom of Bungarus multicinctus, is commonly used for the detection and quantification of nicotinic acetylcholine receptors (nAChR) and anti-nAChR antibodies. A radioiodination method is described which is simple and yields a fully active product which, after fractionation, has a specific activity tenfold higher (900 Ci/mmol) than those routinely used, resulting in enhanced sensitivity of assays and conservation of material, especially muscle extracts.

1984-10-01

33

British Library Electronic Table of Contents (United Kingdom)

Abstract One of the most significant problems in industrial bioprocessing of recombinant proteins using engineered mammalian cells is the phenomenon of cell line instability, where a production cell line suffers a loss of specific productivity (qP). This phenomenon occurs with unpredictable kinetics and has been widely observed in Chinese hamster ovary (CHO) cell lines and with all commonly used gene expression systems. The underlying causes (both genetic and physiological) and the precise molecular mechanisms underpinning cell line instability have yet to be fully elucidated, although recombinant gene silencing and loss of recombinant gene copies have been shown to cause qP loss. In this work we have investigated the molecular mechanisms underpinning qP instability over long-term sub-cult...

2011-01-01

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Monoclonal antibodies: new agents for cancer detection and targeted therapy

Solid-Phase Radioimmunoassay of Total and Influenza-Specific Immunoglobulin G

Antibodies directed against markers on cancer cells are gaining in importance for the purpose of targeting diagnostic and therapeutic agents. In the past, this approach has had very limited success principally because the classical methods for producing antibodies from blood serum of animals immunized with cancer cells or extracts were unsatisfactory. The situation has changed dramatically since 1975 following the design of procedures for 'immortalizing' antibody-producing cells (lymphocytes) by fusing them with cultured myeloma cells to form hybridomas which continuously secrete antibodies. Since these hybridomas produce antibodies coded for by a single antibody-producing cell, the antibodies are called monoclonal. Building on these advances in biomedical research, it is now possible to reproducibly manufacture monoclonal ...

35

M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

An antigen-antibody system of polystyrene tubes coated with immunoglobulin antibody was used for quantitating immunoglobulins. A similar radioimmunoassay method was adapted for a viral antigen-antibody...Full Text Available

1972-02-01

36

Comparative Inhibitory Effects of Antigen and Antibody in the Staphylococcal Enterotoxin Solid-Phase Radioimmunoassay System

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

1989-09-01

37

Antibody avidity in swine lymphocyte antigen-defined miniature pigs.

A solid-phase radioimmunoassay employing ¹²⁵I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies...Full Text Available

1975-06-01

38

Anti-albumin antibodies in chronic liver diseases: diagnostic significance of these antibodies in patients with conventional or immunosuppressive therapy.

Antibody avidity to hen egg white lysozyme (HEWL) was measured by thiocyanate ion elution enzyme-linked immunosorbent assay (ELISA) in swine lymphocyte antigen (SLA) defined miniature pigs. Serum antibody...Full Text Available

1992-10-01

39

Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma

The two categories of anti-albumin antibodies (AAA), namely precipitins (AA-P) and agglutinins (AA-Aggl), were investigated in 260 patients with morphologically diagnosed chronic liver diseases (CLD)....Full Text Available

1981-02-01

40

Amastigote stage-specific monoclonal antibodies against Leishmania major.

SummaryMonoclonal antibodies are standard therapeutics for several cancers including the anti-CD20 antibody rituximab for B cell non-Hodgkin lymphoma (NHL). Rituximab and other...Full Text Available

2010-09-03

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

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Functional expression of anti-hepatitis B virus (HBV) preS2 antigen scFv by cspA promoter system in Escherichia coli and application as a recognition molecule for single-walled carbon nanotube (SWNT) field effect transistor (FET)

Monoclonal antibodies were produced against gamma-irradiated amastigotes of Leishmania major. Five antibodies (T16 through T20) were selected which reacted in enzyme-linked immunoassays with the intracellular...Full Text Available

1989-12-01

42

British Library Electronic Table of Contents (United Kingdom)

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in ...

2010-01-01

43

Urticaria due to Urtica dioica in a neonate

British Library Electronic Table of Contents (United Kingdom)

Urticaria is one of the most common dermatoses during the childhood, but it is very rare in the neonates. A 17-day-old infant with a generalized urticaria was admitted to our pediatric emergency unit. The mother of the infant reported having applied water boiled with stinging nettle (Urtica dioica) onto her nipples twice a day (before and after each breastfeeding) for 2?days in order to heal her nipple cracks. Serum total immunoglobulin E (IgE) and specific IgE levels for stinging nettle were high in the infant and the mother. The rashes began to regress within the first day of the hospitalization and disappeared completely on the second day without treatment. The skin prick test with the water boiled with stinging nettle was positive for the infant with significant induration, but not for...

2011-01-01

44

Pet keeping and dampness in the dwelling: associations with airway infections, symptoms, and physiological signs from the ocular and nasal mucosa:

British Library Electronic Table of Contents (United Kingdom)

Abstract The aim was to utilize data from a study of occupational indoor environments to analyze symptoms and physiological signs in relation to the home environment. A medical investigation was performed at the workplace among university staff (n = 173) from four university buildings in Bergen, in March 2004. Tear film break up time (BUT) was measured by two methods. Nasal patency was measured by acoustic rhinometry. Nasal lavage fluid analysis (NAL) included eosinophilic cationic protein (ECP); myeloperoxidase (MPO), lysozyme and albumin. Atopy was assessed by total serum IgE and specific IgE (Phadiatop). Totally 21%, 21%, 18%, 11%, and 27% had weekly ocular, nasal, facial dermal symptoms, headache and tiredness, respectively, 15% had a damp dwelling, and 20% had a cat or dog. Multiple l...

2007-01-01

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Local and systemic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies.

Human anti-rhesus D IgG1 antibody produced in transgenic plants.

Enzyme-linked immunosorbent assays for the detection of immunoglobulin M (IgM), IgA, IgG1, and IgG2 antibodies against bovine respiratory syncytial virus (BRSV) were used to measure antibody responses...Full Text Available

1987-06-01

46

Heterophyle antibodies causing false positive radio-immunoassay results a case report

Udgivelsesdato: 2002-Apr

2002-01-01

47

Distribution of Borrelia burgdorferi specific antibody among patients with juvenile rheumatoid arthritis in Korea.

A case report of falsely elevated serum hormone values measured by radio-immunoassay (RIA) is described. The radio-immunoassays concerned have a first antibody raised in rabbits and mostly a separation technique based on a second antibody-solid phase system. The presence of heterophyle (anti-rabbit) antibodies in patients' serum is proved.

48

An immunochemical approach to the study of DNA damage and repair

Full Text Available

1993-12-01

49

A Monoclonal Antibody That Specifically Binds Chitosan In Vitro and In Situ on Fungal Cell Walls

The overall objective of this project is to produce antibodies to unique modified DNA bases and develop immunochemical assays to quantitate these lesions in damaged DNA. During this past year we have developed an antibody and chemical test to quantitate a basic sites in DNA and produced antibodies to the 8-oxopurines. This report discusses the detection of a basic sites in DNA and the preparation of antibodies to 8-hydroxyadenine and 8-hydroxyguanine.

1990-11-14

50

Technical Publication Transfer, TO 12R2-2A-1664 Using O'Neil and Associates, Incorporated's Data Supporting: ESC/MSL's MILSTAR Program, MIL-D-28000A (IGES), MIL-M-28001B (SGML), MIL-R-28002A (Raster). Quick Short Test Report.

Full Text Available

2010-08-01

51

Conscan Drive Assembly to 31R2-2FRC181-42 Using ESC/MSL MILSTAR Program's Data: MIL-D-28000A (IGES), MIL-M-28001A (SGML), MIL-D-28003 (CGM). Quick Short Test Report.

The primary objective of the Air Force Continuous Acquisition and Life-cycle Support (CALS) Test Network (AFCTN) is to evaluate the effectiveness of the CALS standards for technical data interchange and to demonstrate the technical capabilities and operat...

1994-01-01

52

Preparation of monoclonal antibodies labelled by astatine

The primary objective of the Air Force Continuous Acquisition and Life-cycle Support (CALS) Test Network (AFCTN) is to evaluate the effectiveness of the CALS standards for technical data interchange and to demonstrate the technical capabilities and operat...

1994-01-01

53

Susceptibility to invasive bacterial infections in children with sickle cell disease

Astatine conversed into cationic form is shown to form stable complex with diethylenetriaminpentacetic acid. Due to this complex astatine joins RN_2 type monoclonal antibodies. More favorable conditions to prepare astatine labelled antibodies are found. Chromatographical analysis and electromigration experiments have shown that astatine is strongly retained in in-vitro biomolecule. Astatine did not escape from the labelled antibodies even in case of urea effect on them. Immune activity of astatine labelled antibodies remained similar in 20 h. 28 refs., 4 figs.

54

British Library Electronic Table of Contents (United Kingdom)

Individuals with sickle cell disease (SCD) demonstrate an increased susceptibility to invasive bacterial infections (IBI). The most common organisms causing IBI are Streptococcus pneumoniae, nontyphi Salmonella species and Haemophilus influenzae type b (Hib). IBI are the most common causes of death in children below 5 years of age with SCD. Increased susceptibility to IBI is because of several factors including dysfunctional antibody production and opsonophagocytosis as well as defective splenic clearance. Early diagnosis of Hib and pneumococcal infections combined with antibiotic prophylaxis and immunization programs, could lead to significant improvements in mortality, especially in Africa. Pediatr Blood Cancer. 2010;55:401-406. Copyright 2010 Wiley-Liss, Inc.

2010-01-01

55

Radioisotopes for radioimmunodetection (RAID) and radioimmunotherapy (RAIT)---current and new perspectives

Estimates of quantitative genetic parameters of immunological traits in the chicken.

In this paper the availability and properties of radioisotopes for both radioimmunodiagnosis (RAID) and radioimmunotherapy (RAIT) are discussed. Examples are provided for radioisotopes available via direct production in nuclear reactors and accelerators or as daughters obtained from radionuclide generator systems whose parents are either reactor or accelerator produced. Important factors which must be considered for the use of a particular radioisotope include availability, the physical half-life and decay properties, and chemical versatility for protein attachment. Although both direct'' and indirect'' methods are available for attachment of radioisotopes to antibodies, this broad field of research is not reviewed in detail. Practical issues related to the availability and use of a variety of radionuclides are described. 47 refs., 5 tabs.

1991-01-01

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Copper-64 Radiopharmaceuticals for PET Imaging of Cancer: Advances in Preclinical and Clinical Research

Three in vivo assays were used to measure the immunocompetence of chickens in two generations of a selection experiment. The obtained data were used to estimate the variance components for sire and dam for antibody production to Pasteurella multocida and Mycoplasma gallisepticum vaccines, for T-cell-mediated immunity evaluated by a phytohemagglutinin wing web assay, and for clearance of foreign particles from the circulatory system. Heritabilities of and genetic correlations among these immunological traits were calculated from the sire variance components. Heritability estimates of the immunological traits based on the sire component of variance ranged from .06 to .53, and genetic correlations among immunological traits were generally negative. PMID:1956847

1991-10-01

57

British Library Electronic Table of Contents (United Kingdom)

Summation Copper-64 (T1/2?=?12.7 hours; ?+, 0.653?MeV [17.8 %]; ??, 0.579?MeV [38.4 %]) has decay characteristics that allow for positron emission tomography (PET) imaging and targeted radiotherapy of cancer. The well-established coordination chemistry of copper allows for its reaction with a wide variety of chelator systems that can potentially be linked to peptides and other biologically relevant small molecules, antibodies, proteins, and nanoparticles. The 12.7-hours half-life of 64Cu provides the flexibility to image both smaller molecules and larger, slower clearing proteins and nanoparticles. In a practical sense, the radionuclide or the 64Cu-radiopharmaceuticals can be easily shipped for PET imaging studies at sites remote to the production facility. Due to the versatility of 64Cu, ...

2009-01-01

58

The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes

The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes

Three cDNAs, designated IIA3, IIA3v, and IIA4, coding for P450s in the CYP2A gene subfamily were isolated from a {lambda}gt11 library prepared from human hepatic mRNA. Only three nucleotide differences and a single amino acid difference, Leu{sup 160}{yields}His, were found between IIA3 and IIA3v, indicating that they are probably allelic variants. IIA4 displayed 94% amino acid similarity with IIA3 and IIA3v. The three cDNAs were inserted into vaccinia virus, and recombinant viruses were used to infect human hepatoma Hep G2 cells. Only IIA3 was able to produce an enzyme that had a reduced CO-bound spectrum with a {lambda}{sub max} at 450 nm. This expressed enzyme was able to carry out coumarin 7-hydroxylation and ethoxycoumarin O-deethylation. cDNA-expressed IIA3v and IIA4 failed to incorporate heme and were enzymatically inactive. Analysis of IIA proteins in human liver microsomes, using antibody against rat IIA2, revealed two proteins of 49 and 50 kDa, the former ...

1990-02-06

59

Evaluation of the effectiveness of an antimicrobial air filter to avoid porcine reproductive and respiratory syndrome virus (PRRSV) aerosol transmission, after 16 months of exposure to a commercial swine environmental conditions

Three cDNAs, designated IIA3, IIA3v, and IIA4, coding for P450s in the CYP2A gene subfamily were isolated from a #lambda#gt11 library prepared from human hepatic mRNA. Only three nucleotide differences and a single amino acid difference, Leu"1"6"0#->#His, were found between IIA3 and IIA3v, indicating that they are probably allelic variants. IIA4 displayed 94% amino acid similarity with IIA3 and IIA3v. The three cDNAs were inserted into vaccinia virus, and recombinant viruses were used to infect human hepatoma Hep G2 cells. Only IIA3 was able to produce an enzyme that had a reduced CO-bound spectrum with a #lambda#_m_a_x at 450 nm. This expressed enzyme was able to carry out coumarin 7-hydroxylation and ethoxycoumarin O-deethylation. cDNA-expressed IIA3v and IIA4 failed to incorporate heme and were enzymatically inactive. Analysis of IIA proteins in human liver microsomes, using antibody against rat IIA2, revealed two proteins of 49 and 50 kDa, the former of ...

1990-02-01

60

Western Blot Analysis of Cereal Grain Prolamins Using an Antibody to Carboxyl-Linked Indoleacetic Acid

The effectiveness of Noveko's antimicrobial filter was evaluated after 16 months of exposure to commercial swine production. The experiment involved the use of a scaled model of a commercial swine facility consisting of 2 small chambers connected by a duct containing the filters. A 5 kg naive pig was placed in the reception chamber for a period of 6 hours after aerosolization with porcine reproductive and respiratory syndrome virus (PRRSV). Blood samples from pigs were collected before and after aerosolization to test for the presence of PRRSV RNA. Only blood samples were tested for PRRSV antibodies by IDEXX 2XR ELISA. None of the 9 pigs tested were found to be infected. The study showed that the technology used to integrate the antimicrobial agent into the filter fibers allows the filter combination to withstand extreme weather and endure commercial swine production for at least 16 months, and can maintain its ...

2010-07-01

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61

Targeting Prostate Cancer Cells In Vivo Using a Rapidly Internalizing Novel Human Single-Chain Antibody Fragment

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor,...Full Text Available

1991-08-01

62

T cell-engaging BiTE antibodies specific for EGFR potently eliminate KRAS- and BRAF-mutated colorectal cancer cells

Human antibodies targeting prostate cancer cell surface epitopes may be useful for imaging and therapy. The objective of this study was to evaluate the tumor targeting of an internalizing human...Full Text Available

2010-03-01

63

Staphylococcal Enterotoxin B: Solid-Phase Radioimmunoassay

Epidermal growth factor receptor (EGFR)-specific monoclonal antibodies predominantly inhibit colorectal cancer (CRC) growth by interfering with receptor signaling. Recent analyses have shown that patients...Full Text Available

2010-07-13

64

Relationship between Legionella spp. and antibody titres at a therapeutic thermal spa in Portugal.

An immunoassay employing ¹²⁵I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated...Full Text Available

1971-11-01

65

Quantitation of Antibody to Non-Hemagglutinating Viruses by Single Radial Hemolysis: Serological Test for Human Coronaviruses

The presence of Legionella spp. in the water of a Portuguese spa was ascertained during the spa season, between May and November. Simultaneously the prevalence of anti-legionella antibodies in people...Full Text Available

1995-08-01

66

Proteome-wide Anti-HCV and Anti-HIV Antibody Profiling for Predicting and Monitoring Response to HCV Treatment in HIV Co-infected Patients

A single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. With the human coronaviruses as models, this test utilizes the binding properties...Full Text Available

1977-06-01

67

Observations on the use of solid-phase-coupled antibodies in the radioimmunoassay of human placental lactogen

We quantified antibody responses to the HCV proteome that are associated with sustained virologic response (SVR) in HIV/HCV co-infected patients treated with pegylated interferon and ribavirin....Full Text Available

2010-09-15

68

Novel Approach for Differential Diagnosis of HIV Infections in the Face of Vaccine-Generated Antibodies

A detailed comparative assessment was made of the use of solid-phase-coupled antibodies in radioimmunoassay, by using an assay for human placental lactogen as a model system. The major advantages of...Full Text Available

1974-03-01

69

Microglial Fc Receptors Mediate Physiological Changes Resulting From Antibody Cross-Linking of Myelin Oligodendrocyte Glycoprotein

SummaryBecause increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine-from virus-induced antibodies. Most of the currently...Full Text Available

2006-11-01

70

In vitro antigen-induced antibody responses to hepatitis B surface antigen in man. Kinetic and cellular requirements.

Antibodies to myelin oligodendrocyte glycoprotein (MOG) have been implicated in Multiple Sclerosis demyelination through activation of complement and/or macrophage-effector processes. We presented...Full Text Available

2008-05-30

71

Evaluation of Multiplexed Fluorescent Microsphere Immunoassay for Detection of Autoantibodies to Nuclear Antigens

In this report we define the parameters of the human immune response after immunization with hepatitis B vaccine. 2 wk after booster immunization, there is significant spontaneous secretion of antibody...Full Text Available

1984-10-01

72

Dried blood spots as a source of anti-malarial antibodies for epidemiological studies

Antibodies to extractable nuclear antigens (ENA) are found in a variety of collagen vascular diseases. Determining the individual specificities of these antibodies is extremely useful in establishing...Full Text Available

2004-11-01

73

Development and Application of Pathovar-Specific Monoclonal Antibodies That Recognize the Lipopolysaccharide O Antigen and the Type IV Fimbriae of Xanthomonas hyacinthi

BackgroundBlood spots collected onto filter paper are an established and convenient source of antibodies for serological diagnosis and epidemiological surveys. Although recommendations...Full Text Available

74

Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.

The objective of this study was to develop a specific immunological diagnostic assay for yellow disease in hyacinths, using monoclonal antibodies (MAbs). Mice were immunized with a crude cell wall preparation...Full Text Available

1999-09-01

75

Cryptosporidium parvum (Apicomplexa: Cryptosporidiidae) oocyst and sporozoite antigens recognized by bovine colostral antibodies.

A serological trapping technique for detecting rotaviruses is described which involves coating electron microscope grids with protein A and specific rotavirus antiserum. The presence of a layer of antibodies...Full Text Available

1980-07-01

76

Clinical Significance of the Detection of Antinuclear Antibodies in Patients with Acute Hepatitis A

Colostral whey from seven hyperimmunized and two control cows (hyperimmune bovine colostrum) was examined by Western immunoblotting for the presence of antibody against oocysts and sporozoites of Cryptosporidium...Full Text Available

1990-09-01

77

Circulating antibody to myelin basic protein in relapsing-remitting multiple sclerosis? A comparative group and sequential study by radioimmunoassay.

Background/AimsThe findings of several recent studies suggest that antinuclear antibodies (ANAs) are frequently detected in patients with acute hepatitis A (AHA). However, the clinical...Full Text Available

2011-09-01

78

Cerebellar cell surface antigens of mouse brain.

Sera from multiple sclerosis patients with relapsing-remitting disease and normal subjects were tested for antibody to myelin basic protein by a sensitive radioimmunoassay. The results showed a marginally...Full Text Available

1978-12-01

79

Antibody protects against lethal infection with the neurally spreading reovirus type 3 (Dearing).

Reaggregated cells from 6- to 8-day-old mouse cerebella have been used to raise antibodies in rabbits. The interaction of these antibodies with cerebellar cell surface components was assessed by cytotoxicity...Full Text Available

1975-10-01

80

Antibodies against gonadotropin-releasing hormone (GnRH) and destruction of enteric neurons in 3 patients suffering from gastrointestinal dysfunction

The mammalian reoviruses have provided a valuable model for studying the pathogenesis of viral infections of the central nervous system (CNS). We have used this model to study the effect of antibody...Full Text Available

1988-12-01

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81

Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating

BackgroundAntibodies against gonadotropin-releasing hormone (GnRH) and gastrointestinal dysmotility have been found after treatment with GnRH analogues. The aim of this study was...Full Text Available

82

A naturally occurring human antibody to loops of Henle

Botulism is caused by the botulinum neurotoxins (BoNTs), the most poisonous substance known. Because of the high potency of BoNT, development of diagnostic and therapeutic antibodies for botulism requires...Full Text Available

2010-04-01

83

A double antibody solid phase assay for DNA autoantibodies for clinical use.

A hitherto unreported immunofluorescent staining pattern obtained from human sera is described. Serum from six patients was found to possess specific antibody activity against lining cells of loops...Full Text Available

1973-08-01

84

Fine mapping of a foot-and-mouth disease virus epitope recognized by serotype-independent monoclonal antibody 4B2.

DNA antoantibodies in serum will bind to antigen-coated polystyrene tubes and can be detected by radiolabelled anti-immunoglobulin. The method is quantitative, gives information on the antibody class...Full Text Available

1976-08-01

85

Effects of chelate rigidity and density on the in vivo performance of [sup 203]Pb and [sup 88]Y labeled monoclonal antibodies

Full Text Available

2011-02-01

86

Technical Publication Transfer Using O'Neil and Associates' Data Supporting ESC/MSL MILSTAR Program T O 31R2-2U-433: MIL-STD-1840A, MIL-D-28000A (IGES), MIL-M-28001B (SGML), MIL-R-28002A (Raster), MIL-D-28003 (CGM). Quick Short Test Report.

Short communication.

1993-01-01

87

Technical Publication Transfer T O 31R2-2U-432 Using O'Neil and Associates, Inc. Data Supporting ESC/MSL MILSTAR Program: MIL-STD-1840A, MIL-D-28000A (IGES), MIL-M-28001A (SGML), MIL-R-28002A (Raster), MIL-D-28003 (CGM). Quick Short Test Report.

The primary objective of the Air Force Continuous Acquisition and Life-Cycle Support (CALS) Test Network (AFCTN) is to evaluate the effectiveness of the CALS standards for technical data interchange and to demonstrate the technical capabilities and operat...

1994-01-01

88

Technical Publication TO 12R2-2ARC208-113 Transfer Using O'Neil and Associates' Data Supporting ESC/MSL's MILSTAR Program: MIL-STD-1840A, MIL-D-28000A (IGES), MIL-M-28001B (SGML), MIL-R-28002A (Raster), MIL-D-28003 (CGM). Quick Short Test Report.

The primary objective of the Air Force Continuous Acquisition and Life-cycle Support (CALS) Test Network (AFCTN) is to evaluate the effectiveness of the CALS standards for technical data interchange and to demonstrate the technical capabilities and operat...

1994-01-01

89

Technical Manual Transfer TO 31R2-2FRC181-42 Using Raytheon Company's Data Supporting ESC/MSK's MILSTAR Program: MIL-STD-1840A, MIL-D-28000A (IGES), MIL-M-28001A (SGML), MIL-D-28003 (CGM). Quick Short Test Report.

The primary objectives of the Air Force Continuous Acquisition and Life-cycle Support (CALS) Test Network (AFCTN) is to evaluate the effectiveness of the CALS standards for technical data interchange and to demonstrate the technical capabilities and opera...

1994-01-01

90

Sensitive enzyme immunoassay for detecting immunoglobulin M antibodies to Sindbis virus and further evidence that Pogosta disease is caused by a western equine encephalitis complex virus.

The primary objective of the Air Force Continuous Acquisition and Life-cycle Support (CALS) Test Network (AFCTN) is to evaluate the effectiveness of the CALS standards for technical data interchange and to demonstrate the technical capabilities and operat...

1994-01-01

91

Reduced "9"9"mTc labelled NCA-95/CEA-antibody uptake in liver due to gentle antibody reconstitution

An antibody capture enzyme immunoassay (EIA) was adapted for the detection of immunoglobulin M (IgM) antibody to Sindbis (SIN) virus. Sera from humans with a febrile illness characterized by rash and...Full Text Available

1985-10-01

92

Preparation and Properties of Antibodies against Indoleacetic Acid (IAA)-C5-BSA, a Novel Ring-Coupled IAA Antigen, as Compared to Two Other Types of IAA-Specific Antibodies ¹

The influence of reconstituting a murine monoclonal IgG_1 antibody kit with pertechnetate Tc99m on antibody distribution in the liver, spleen and sternal bone marrow of patients was examined. The "9"9"mTc-labelled antibody used is directed against non-specific cross-reacting antigen (NCA-95) and carcinoembryonic antigen (CEA) and has been successfully applied for imaging tissue inflammation and bone marrow scanning. Radioactivity uptake was determined in the liver, spleen, bone marrow and a precordial background region in a consecutive series of 25 patients, examined with an antibody preparation, routinely radiolabelled according to the manufacturer's recommendations and in 14 patients, in whom the antibody was reconstituted with special care, avoiding bubble formation and dropping of buffer into the antibody-containing vial. Gentle compared with routine ...

93

Near-Infrared Fluorescence Labeled Anti-TAG-72 Monoclonal Antibodies for Tumor Imaging in Colorectal Cancer Xenograft Mice

Two types of indoleacetic acid (IAA) antigens have been described in the literature which show marked differences with respect to antibody specificity. In this communication an alternative antigen design...Full Text Available

1989-04-01

94

Monoclonal antibody imaging in malignant and benign gastrointestinal diseases

Anti-TAG-72 monoclonal antibodies target the tumor-associated glycoprotein (TAG)-72 in various solid tumors. This study evaluated the use of anti-TAG-72 monoclonal antibodies, both murine CC49...Full Text Available

2009-01-01

95

Molecular basis of factor VIII inhibition by human antibodies. Antibodies that bind to the factor VIII light chain prevent the interaction of factor VIII with phospholipid.

The "1"3"1I-labelled monoclonal antibodies 791T/36 and 79IT/36 Fab fragments have been evaluated in the imaging of patients with colorectal cancer, with benign colorectal tumours, and with malignant tumours of other parts of the gastrointestinal tract. The results of clinical imaging have been carefully correlated with clinical findings and direct measurement of the preferential uptake of antibody by the tumours. (UK).

96

Comparison of indirect immunofluorescent-antibody assay, enzyme-linked immunosorbent assay, and Western immunoblot for the diagnosis of Lyme disease in dogs.

Most antibodies to factor VIII have recently been shown to react with discrete regions of the factor VIII light chain (within the C2 domain) and/or the factor VIII heavy chain (within the amino-terminal...Full Text Available

1989-06-01

97

Comparative Study of ¹²⁵I- and [³H]Acetate-Labeled Antibodies in Detecting Iridescent Viruses

Enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescent-antibody assay (IFA), and Western immunoblot were used to test serum samples from 128 dogs for the presence of antibody to Borrelia...Full Text Available

1990-01-01

98

Increased expression after x-irradiation of MUC1 in cultured human colon carcinoma HT-29 cells

Radioimmunoassays for detecting cell-associated or released virus are described using either ¹²⁵I- or [³H]acetate-labeled antibodies. In the first assay system, antigen-antibody...Full Text Available

1974-07-01

99

B chain is a functional subunit of beta-bungarotoxin for inducing apoptotic death of human neuroblastoma SK-N-SH cells.

The effect of X-irradiation on production of MUC1 was studied with human colon carcinoma HT-29 cells. As evaluated by immunocytochemical staining, the percentages or MUC1-positive cells in cells at 4 days after 6 Gy irradiation and in unirradiated control cells were 52#+-#3.5% (n=6) and 26#+-#2.8% (n=6), respectively. Flow-cytometric analysis of living cells showed that MUC1 began to rise from day 1, reaching a plateau by day 4 after 6 Gy irradiation. Western blot analysis with monoclonal antibody MY.1E12 against glycosylated MUC1 (mature form) showed dose-dependent increases of two bands (500 and 390 kDa) corresponding to two polymorphic MUC1 alleles. Premature forms of MUC1 (350 and 240 kDa) were detectable with monoclonal antibody HMFG-2 only in irradiated cells, suggesting that new core protein synthesis had been induced. The transcriptional activity of the MUC1 gene was analyzed in terms of transient expression of ...

2000-03-01

100

Rocky Mountain spotted fever in areas of high and low prevalence: survey for canine antibodies to spotted fever rickettsiae.

beta-Bungarotoxin (beta-Bgt), a presynaptic phospholipase A(2) (PLA(2)) neurotoxin isolated from the venom of Bungarus multicinctus, consists of A chain and B chain. The goal of the present study is to explore the functional contribution of the two subunits to the toxicity of beta-Bgt. beta-Bgt was found to induce apoptotic death of SK-N-SH cells via elevating intracellular Ca(2+) and intracellular ROS production. Moreover, an activation of p38 MAPK was associated with the cytotoxicity of beta-Bgt. SB202190 (p38 MAPK inhibitor), N-acetylcysteine (antioxidant reagent), 1,2-bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid (BAPTA) (Ca(2+) chelator) and the inhibitors of Ca(2+) release from intracellular depots (ruthenium red and 2-aminoethoxydiphenyl borate) effectively attenuated the cytotoxicity of beta-Bgt. In sharp contrast to the inability of A chain, B chain was able to induce cytotoxic effects on SK-N-SH cells as beta-Bgt did. Abolishment of PLA(2) activity ...

2007-10-13

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101

Role of the Yersinia pestis Ail Protein in Preventing a Protective Polymorphonuclear Leukocyte Response During Bubonic Plague.

Antibodies to Rickettsia rickettsii were detected by indirect immunofluorescence in sera from 149 of 467 dogs (32%) examined from 4 military installations located in Kentucky, North Carolina, Pennsylvania, and Virginia. The prevalence at individual installations ranged from 4.3% at Fort Knox, Ky, to 63.4% at Fort Bragg, NC. Most of the seropositive dogs were in the working and sporting groups of dogs. The difference in antibody prevalence between sexes was not significant. Serologic responses were related to R rickettsii infection, although antibodies to R montana also were detected in a few of the sera. Comparison of serodiagnostic methods indicated that the indirect fluorescent antibody test was more sensitive than was the indirect hemagglutination test for obtaining survey data on the prevalence of Rocky Mountain spotted fever in the area. PMID:6808878

1982-08-01

102

Nod2 sensing of lysozyme-digested peptidoglycan promotes macrophage recruitment and clearance of S. pneumoniae colonization in mice.

The ability of Yersinia pestis to forestall the mammalian innate immune response is a fundamental aspect of plague pathogenesis. In this study, we examined the effect of Ail, a 17-kDa outer membrane protein that protects Y. pestis against complement-mediated lysis, on bubonic plague pathogenesis in mice and rats. The Y. pestis ail mutant was attenuated for virulence in both rodent models. The attenuation was greater in rats than in mice, which correlates with the ability of normal rat serum, but not mouse serum, to kill ail-negative Y. pestis in vitro. Intradermal infection with the ail mutant resulted in an atypical, subacute form of bubonic plague associated with extensive recruitment of polymorphonuclear leukocytes (PMN or neutrophils) to the site of infection in the draining lymph node and the formation of large purulent abscesses that contained the bacteria. Systemic spread and mortality were greatly attenuated, however, and a productive adaptive immune ...

2011-10-01

103

Visualization of Tumor Angiogenesis Using MR Imaging Contrast Agent Gd-DTPA-anti-VEGF Receptor 2 Antibody Conjugate in a Mouse Tumor Model.

Streptococcus pneumoniae colonizes the mucosal surface of the human upper respiratory tract. A colonization event is gradually cleared through phagocytosis by monocytes/macrophages that are recruited to the airway lumen. Here, we sought to define the bacterial and host factors that promote monocyte/macrophage influx and S. pneumoniae clearance using intranasal bacterial challenge in mice. We found that the recruitment of monocytes/macrophages required their expression of the chemokine receptor CCR2 and correlated with expression of the CCR2 ligand CCL2. Production of CCL2 and monocyte/macrophage recruitment were deficient in mice lacking digestion of peptidoglycan by lysozyme (LysM) and cytosolic sensing of the products of digestion by Nod2. Ex vivo macrophages produced CCL2 following bacterial uptake, digestion by LysM, and sensing of peptidoglycan by Nod2. Sensing of digested peptidoglycan by Nod2 also required the pore-forming toxin ...

2011-08-15

104

The inhibition of the primary interaction between "1"2"5I-labeled human hemoglobin and rabbit anti-human hemoglobin sera: a sensitive radioimmunoassay technique

Full Text Available

2010-07-01

105

... ammonium compounds antigen-antibody reactions hemoglobin inhibition

106

Reversing B cell aging

Postpartum spontaneous colonic perforation due to antiphospholipid syndrome

Age-related alterations in the cellular composition of the B lineage are a major cause of the poor antibody response to vaccination and to infectious agents among the elderly population. The mechanisms...Full Text Available

107

Neurobrucellosis in Stranded Dolphins, Costa Rica

The antiphospholipid syndrome (APS) is a multi-systemic disease being characterized by the presence of antiphospholipid antibodies that involves both arterial and venous systems resulting in arterial...Full Text Available

2009-01-28

108

Isolation of antibodies specific to sickle hemoglobin by affinity chromatography using a synthetic peptide

Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. Brucella...Full Text Available

2008-09-01

109

Incidence and specificity of antibodies to types I, II, III, IV, and V collagen in rheumatoid arthritis and other rheumatic diseases as measured by 125I-radioimmunoassay

Antibodies to hemoglobin have been studied with a radioimmunoassay which employs ["1"4C]carbamylated (= carbamoylated) hemoglobin S. An antiserum raised against hemoglobin S, which initially discriminated poorly between hemoglobins S and A, was fractionated by absorption to a column of Sepharose to which a synthetic peptide corresponding to the first 13 amino-acid residues of the #beta# chain of sickle hemoglobin had been covalently bound. A subpopulation of the antiserum was eluted from this column with 4 M guanidine . HCl. These antibodies showed binding to hemoglobin S but not to hemoglobin A and this interaction could be inhibited by the synthetic peptide. These antibodies, of demonstrated fine structural specificity, may be useful in the detection of sickle hemoglobin and in the study of its structure in solution.

110

Human heterophilic antibodies against equine immunoglobulins: assessment of their role in the early adverse reactions to antivenom administration

Antibodies to human native and denatured types I, II, III, IV, and V collagens were measured using 125I-radioimmunoassay. Mean levels of binding by sera from 30 rheumatoid arthritis patients were significantly higher than those from 20 normal subjects against all of the collagens tested. The relative antibody concentration was higher in synovial fluid than in simultaneously obtained serum. Many patients with gout or various other rheumatic diseases also had detectable anticollagen antibodies. With a few notable exceptions, the majority of the reactivity detected in all patient groups was directed against covalent structural determinants present on all of the denatured collagens, suggesting a secondary reaction to tissue injury.

111

British Library Electronic Table of Contents (United Kingdom)

Summary The presence of human heterophilic antibodies against horse immunoglobulins (HHA-HI) was determined by ELISA in sera from healthy volunteers and from patients who received equine antivenom for therapy of snake bite envenoming. These patients were selected from two independent clinical studies: one in Colombia in which patients received antivenom constituted by whole IgG (n=25); and the other in Brazil where an antivenom constituted by F(abprime)2 fragments was administered (n=31). Results show that healthy volunteers have antibodies, mainly of the IgG class, able to react with whole equine IgG. Additionally, patients have IgG antibodies that react both with whole equine IgG and F(abprime)2 fragments. In both clinical studies, no significant differences were observed in the HHA-HI t...

2008-01-01

112

First report on antibody response of Seriola dumerilii (Risso 1810) challenged with Listonella anguillarum

British Library Electronic Table of Contents (United Kingdom)

The greater amberjack, Seriola dumerilii (Risso 1810) is a semi-pelagic fish and a worldwide species; it is considered a promising candidate for the diversification of Mediterranean aquaculture. In this paper an experimental injection with Listonella (Vibrio) anguillarum was performed to study the immune response of S. dumerilii. Antibody titres to L. anguillarum O1 were determined with indirect-ELISA at different times over a period of 42 days. Results showed that the antibody levels against L. anguillarum were significantly higher in the challenged fish compared to the control. They started developing since the 5th day reaching the highest peak on day 20 after injection, indicating a fast response of the immune system. The observed antibody titre was very high versus L. anguillarum if co...

2008-01-01

113

Apparatus for use in radioimmunoassays

Antibodies from malaria-exposed pregnant women recognize trypsin resistant epitopes on the surface of Plasmodium falciparum-infected erythrocytes selected for adhesion to chondroitin sulphate A

Apparatus for solid-phase antibody separation techniques used in radioimmunoassays is described in this invention. It consists of a rectangular prism tray with multiple wells protruding into its interior from one side. Near the base of the tray is an orifice used for creating evacuated condition within the structure. At the base of each well there is an orifice of such size and shape as to retain an aqueous liquid under given pressure conditions but permit the evacuation of this liquid at reduced pressure. The outlet of these orifices is in the shape of an inverted conical frustrum. Each of the wells contains an antibody coated disc of porous cellulose paper surrounded by a plastic support. The porous nature of the cellulose paper ensures contact between the antibody coating and the antigen. The use of antibody coated porous cellulose paper in combination with the vacuum operated apparatus simplifies ...

1991-08-26

114

A search for the epidemic typhus agent in Ethiopian ticks*

Udgivelsesdato: 2004-Sep-6

2004-01-01

115

A COMPARISON OF FOUR FLUORESCENT ANTIBODY-BASED METHODS FOR PURIFYING, DETECTING, AND CONFIRMING CRYPTOSPORIDIUM PARVUM ...

The presence of antibodies to Rickettsia prowazeki in domestic animals from several parts of Africa, and the isolation of this rickettsia from the blood of goats and sheep and from...Full Text Available

1973-01-01

116

... r2 = 0.9) in the data was a power model where the number of filters was equal to ... ...

117

An ELISA-based high throughput protein truncation test for inherited breast cancer

Enteropathogenic E. coli were sought routinely by the fluorescent antibody technique, using monovalent and polyvalent conjugates (rhodamine sulfonyl· chloride, fluorescein and rhodamine isothiocyanate)....Full Text Available

1962-11-03

118

Experimental bovine trypanosomiasis. Changes in serum immunoglobulins, complement and complement components in infected animals.

IntroductionBreast cancer is the most diagnosed and second leading cause of cancer deaths in the U.S. female population. An estimated 5 to 10 percent of all breast cancers are inherited, caused by mutations in the breast cancer susceptibility genes (BRCA1/2). As many as 90% of all mutations are nonsense mutations, causing a truncated polypeptide product. A popular and low cost method of mutation detection has been the protein truncation test (PTT), where target regions of BRCA1/2 are PCR amplified, transcribed/translated in a cell-free protein synthesis system and analyzed for truncated polypeptides by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. We previously reported a novel High Throughput Solid-Phase PTT (HTS-PTT) based on an enzyme-linked immunosorbent assay (ELISA) format that eliminates the need for radioactivity, SDS-PAGE and subjective interpretation of the results. Here, we report the next generation HTS-PTT ...

2010-10-04

119

Effects of dietary glucosylceramide on dermatitis in atopic dermatitis model mice

In three calves experimentally infected with Trypanosoma congolense the amounts of IgG1 and IgG2 were little changed and similar to those of normal animals. IgM increased in amount early in the infection and the amount of the increase appeared related to the parasite burden. The amounts of IgA and IgE were both much decreased and this also appeared related to the numbers of parasites in the blood. There was a decrease in the amounts of total haemolytic complement and complement components C1, C1q and C3 in the infected calves. Furthermore the amounts of properdin fluctuated with the cyclical changes in numbers of T. congolense parasites in the individual calves. No significant change in the amount of C8 was observed. It is considered that activation of both the alternative and the classical complement pathways occurs in trypanosome infected animals but that neither pathway goes to its terminal stages.

1978-11-01

120

British Library Electronic Table of Contents (United Kingdom)

The effects of dietary plant and yeast cerebroside (glucosylceramide), a major sphingolipid in plants and yeast, on atopic dermatitis (AD) like symptoms were investigated in a mouse model. After 7 wk of feeding with a diet containing maize glucosylceramide, plasma IgE levels became significantly lower and in contrast, the levels of interleukin (IL)-12, which induces cellular immunity, became significantly higher in the AD mice than in the controls. However, the sphingolipid constituents of the skin fraction in the maize glucosylceramide fed group did not contain sphingoid bases of plant origin, such as 8-unsaturated sphingoid bases. The results of the present study indicated that dietary plant glucosylceramide prevented AD-like symptoms in AD model mice via regulation of Th1/Th2 balance. P...

2010-01-01

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121

On the effect of ionizing radiation on some factors of hamster natural immunity in norm and in himenolepis diminuta invasion

No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls

Effect of single and chronic irradiation on factors of hamsters natural immunity such as complement, lysozyme and antibodies in norm and in the cestode invasion has been investigated. Significant phase changes in the complement activity level, lysozyme content and specific antibodies tite have been shown to occur in hamster blood under the influence of ionizing radiation. Cestode invasion in irradiated host organism increases the negative effect of ionizing radiation on the hamster immyne system.

1994-01-01

122

British Library Electronic Table of Contents (United Kingdom)

New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env or the XMRV-gag antigen.

2011-01-01

123

Monoclonal antibodies to ovine SBU-T8 and SBU-T6 bind analogous molecules on bovine lymphocytes.

Double-antibody solid-phase radioimmunoassay: a simplified phase-separation procedure applied to various ligands

Monoclonal antibodies (mAb) to ovine T-lymphocyte molecules SBU-T8 (Maddox, Mackay & Brandon, 1985), the cytotoxic T lymphocyte, human CD8 equivalent, and SBU-T6 (Mackay et al., 1985), the immature...Full Text Available

1989-06-01

124

Double-antibody solid-phase radioimmunoassay: a simplified phase-separation procedure applied to various ligands

The purpose was to develop a simplified and reliable method of separating free from antibody-bound ligand using a precipitating antibody linked to a cellulose derivative. Dose-response curves and control sera were set up in parallel for various pituitary and placental polypeptides, steroid hormones, insulin, glucagon, triiodothyronine, thyroxine, angiotensin I, calcitonin, gastrin, cyclic AMP, and digoxin. After first-antibody reactions had reached equilibrium, free and bound ligand were separated using a double-antibody solid-phase system in parallel with conventional methods, including dextran-coated charcoal, double-antibody precipitation, single-antibody solid phase, organic solvents, salt precipitation, and anion-exchange resins. The effect of variations in temperature, incubation time, protein content, pH, and amount of separating material added were ...

1980-06-01

125

DIMINISHED DEGRADATION OF MYELIN BASIC PROTEIN BY ANTI-SULFATIDE ANTIBODY AND INTERFERON-? IN MYELIN FROM GLIA MATURATION FACTOR-DEFICIENT MICE

The purpose was to develop a simplified and reliable method of separating free from antibody-bound ligand using a precipitating antibody linked to a cellulose derivative. Dose-response curves and control sera were set up in parallel for various pituitary and placental polypeptides, steroid hormones, insulin, glucagon, triiodothyronine, thyroxine, angiotensin I, calcitonin, gastrin, cyclic AMP, and digoxin. After first-antibody reactions had reached equilibrium, free and bound ligand were separated using a double-antibody solid-phase system in parallel with conventional methods, including dextran-coated charcoal, double-antibody precipitation, single-antibody solid phase, organic solvents, salt precipitation, and anion-exchange resins. The effect of variations in temperature, incubation time, protein content, pH, and amount of separating material added were ...

126

Antibodies against a novel nucleolar and cytoplasmic antigen (p105-p42) present in the sera of patients with a subset of rheumatoid arthritis (RA) with signs of scleroderma

In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin...Full Text Available

2007-06-01

127

Development of a new radiolabel (lead-203) and new chelating agents for labeling monoclonal anntibodies for imaging

We identified three patients (two of them relatives) with RA and signs of scleroderma whose sera contained a high titre of IgG class antibodies against the nucleoli and the nucleoplasm of cells of different...Full Text Available

1998-11-01

128

Limitation of immunohistochemical localization of estrogen receptor

High liver uptake and slow body clearance presently limit the usefulness of "1"1"1In labeled antibodies for tumor imaging. We have investigated "2"0"3Pb as an alternate and better antibody label. The DTPA and cyclohexyl EDTA (CDTA) conjugates of an anticolon carcinoma antibody, 17-1A were labeled (bicyclic anhydride method) with "2"0"3Pb and "1"1"1In with 60 and 90% labeling yields, respectively. The biodistribution of "2"0"3Pb-17-1A conjugates was compared with the corresponding "1"1"1In-labeled preparations and with "2"0"3Pb-DTPA, "2"0"3Pb-nitrate and nonrelevant antibody controls in normal and human tumor (SW948) xenografted nude mice at 24, and 96 hr. Lead-203-labeled CDTA and DTPA antibody conjugates gave similar in vivo distributions. Even though the lead bound to these chelate-antibody conjugates was more labile in serum and in vivo, compared to indium, ...

1988-05-22

129

Pre-therapy 18F-FDG PET quantitative parameters help in predicting the response to radioimmunotherapy in non-Hodgkin lymphoma

Although the radioreceptor method is widely used for estrogen receptor assay in human tissue, it has several limitations and a number of alternative methods are being explored. An immunohistochemical method of estrogen receptor assay using a specific antibody to estradiol has been proposed as a suitable alternative. The present study was designed to evaluate the validity of this method in detecting true estrogen receptors in human tumor tissue. Using radioiodinated antibody to estrogen, we have demonstrated that the estrogen antibody can detect the estrogen when it is bound to 4S type receptor but is unable to bind to estrogen when the hormone is bound to 8S type receptor. Our observations suggest that the immunohistochemical method of detection of intracellular cytosolic receptor for estrogen is not a suitable alternative to the currently used radioreceptor method.

1986-10-01

130

The first international standard for antibodies to double stranded DNA.

Radioimmunotherapy (RIT) is a new treatment option for patients with non-Hodgkin lymphoma (NHL). Response to RIT currently remains difficult to predict using conventional prognostic factors and could be refined using functional imaging. The goal of this work is to evaluate the value of 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) in predicting response to Yttrium 90-labeled monoclonal antibodies for patients with NHL. Thirty-five patients with NHL who had undergone 18F-FDG PET prior to RIT with either 90Y-ibritumomab tiuxetan (group A; n=17) or 90Y-epratuzumab tetraxetan (group B; n=18) were included in this retrospective study. Four functional criteria were determined for each tumour lesion in a given patient: maximum and mean standard uptake values (SUVmax and SUVmean), functional lesion volume (LVol) and total lesion glycolysis (TLG, product of the volume and the SUVmean). For each patient, we determined highest SUVmax and ...

2010-03-01

131

The effect of an ?-globulin preparation and of polyribonuclease complexes on humoral antibody formation

This paper announces the availability of the first international standard for anti-double-stranded DNA (anti-dsDNA). The material, coded Wo/80, was obtained after recalcification of plasma taken from...Full Text Available

1988-09-01

132

The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination

The effects of bovine α-globulin (BAG), bovine-ribonuclease serum albumin (BSA-RNase) and polyribonuclease (poly-RNase) complexes on the primary response of rodents to sheep erythrocytes have...Full Text Available

1968-06-01

133

Tat-Neutralizing Antibodies in Vaccinated Macaques

Activation-induced deaminase (AID) initiates somatic hypermutation, gene conversion and class switch recombination by deaminating variable and switch region DNA cytidines to uridines. AID is predominantly...Full Text Available

134

Spontaneous coronary artery dissection in the postpartum period: association with antiphospholipid antibody

The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade...Full Text Available

2003-03-01

135

Serum Concentrations of Antibodies Against Vaccine Toxoids in Children Exposed Perinatally to Immunotoxicants

Spontaneous coronary artery dissection (SCAD) is an extremely uncommon cause of myocardial infarction, occurring predominantly in women during or after pregnancy. The exact aetiology...Full Text Available

2004-09-01

136

Scarce resources for nuclear... [Disaster Med Public Health Prep...

BackgroundPolychlorinated biphenyls (PCBs) may cause immunotoxic effects, but the detailed dose–response relationship and possible vulnerable time windows of exposure are...Full Text Available

2010-10-01

137

Safety and immunogenicity of a vaccine bait containing ERA strain of attenuated rabies virus.

and antibody responses of rhesus macaques exposed to the human gammaretrovirus XMRV. J Virol. 2011 May ;85(9):4547-57. Epub 2011 Feb 16 . PubMed Your browsing activity is...

2011-10-15

138

Role of IgG and complement component C5 in the initial course of experimental cryptococcosis.

Ninety percent of foxes fed commercial ERA vaccine in a specially designed bait developed rabies serum neutralizing antibodies. The vaccine bait did not cause clinical signs of rabies when consumed...Full Text Available

1987-10-01

139

Risk factors for thrombosis in lupus patients.

Although cellular immunity has a crucial role during cryptococcosis, several in vitro studies have pointed out the importance of IgG anti-Cryptococcus neoformans antibodies and complement components...Full Text Available

1989-12-01

140

Predictive and prognostic markers for epidermal growth factor receptor inhibitor therapy in non-small cell lung cancer

Lupus anticoagulant, concentrations of anticardiolipin antibodies, antithrombin III, plasminogen, (free) protein S, protein C, prothrombin, platelet counts, and bleeding times were determined in 74...Full Text Available

1989-11-01

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141

Passive immunity to feline leukemia: evaluation of immunity from dams naturally infected and experimentally vaccinated.

Epidermal growth factor receptor (EGFR) related therapies – mainly tyrosine kinase inhibitors (TKIs) such as erlotinib and gefitinib, but also monoclonal antibodies targeting EGFR, for example,...Full Text Available

2009-11-01

142

Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation.

Antibodies against feline leukemia virus (FeLV) and the feline oncornavirus-associated cell membrane antigen (FOCMA) were transferred from pregnant cats to their suckling kittens. All of these kittens...Full Text Available

1977-04-01

143

Muscle-Specific Calpain is Localized in Regions Near Motor Endplates in Differentiating Lobster Claw Muscles

A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its...Full Text Available

1975-03-01

144

Lymphoma depletion during CD20 immunotherapy in mice is mediated by macrophage Fc?RI, Fc?RIII, and Fc?RIV

Calpains are Ca²⁺-dependent proteinases that mediate protein turnover in crustacean skeletal muscles. We used an antibody directed against lobster muscle-specific calpain (Ha-CalpM)...Full Text Available

2007-11-01

145

Low-frequency sound transmission through a g... [J Acoust Soc...

Despite the demonstrated clinical efficacy of CD20 monoclonal antibody (mAb) for lymphoma therapy, the in vivo mechanisms of tumor depletion remain controversial and variable. To identify the molecular...Full Text Available

2008-08-15

146

Location of an epitopic site on epiglycanin by molecular immunoelectron microscopy.

and antibody responses of rhesus macaques exposed to the human gammaretrovirus XMRV. J Virol. 2011 May ;85(9):4547-57. Epub 2011 Feb 16 . PubMed Surgical staging of early...

2011-10-15

147

Increased prevalence of antibodies to enteropathogenic Yersinia enterocolitica virulence proteins in relatives of patients with autoimmune thyroid disease

Antibodies of the IgM type present in rabbit anti-epiglycanin antiserum were purified by (NH4)2SO4 precipitation and by ion-exchange, affinity and gel-filtration chromatography. After papain treatment...Full Text Available

1985-04-01

148

Increased prevalence of Borrelia burgdorferi infections in Bernese Mountain Dogs: a possible breed predisposition

Infections have been implicated in the pathogenesis of a number of autoimmune diseases, and Yersinia enterocolitica (YE) might play a role in the development of autoimmune thyroid disease...Full Text Available

2003-05-01

149

In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.

BackgroundGlomerulonephritis in dogs has been associated with B. burgdorferi infections. In Bernese Mountain Dogs with glomerulonephritis antibodies against B....Full Text Available

150

Immunoreactive properties of anti-thyroglobulin autoantibodies isolated by affinity chromatography from human thyroiditis serum.

An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly...Full Text Available

1986-08-01

151

Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an Avena sativa Plasma Membrane Determinant 1

A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed...Full Text Available

1978-02-01

152

Immunization of foxes by the intestinal route using an inactivated rabies vaccine.

An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target...Full Text Available

1992-01-01

153

Identification of the Neoplastically Transformed Cells in Marek's Disease Herpesvirus-Induced Lymphomas: Recognition by the Monoclonal Antibody AV37

Approximately 30% of foxes given two doses of an inactivated rabies antigen delivered directly into the intestinal tract developed an immune response as measured by rabies serum neutralizing antibodies....Full Text Available

1989-01-01

154

Identification of circulating neuropilin-1 and dose-dependent elevation following anti-neuropilin-1 antibody administration

Understanding the interactions between herpesviruses and their host cells and also the interactions between neoplastically transformed cells and the host immune system is fundamental to understanding...Full Text Available

2002-07-01

155

Hepatitis B antigen and antibody in the blood of prostitutes visiting an outpatient venereology department in Rotterdam.

Neuropilin-1 (NRP1) acts as a co-receptor for class 3 semaphorins and vascular endothelial growth factor and is an attractive angiogenesis target for cancer therapy. In addition to the transmembrane...Full Text Available

2009-07-01

156

From antibody insult to fibrosis in neonatal lupus - the heart of the matter

We took blood samples from 128 prostitutes visiting the outpatient venereology department of the University Hospital, Rotterdam-Dijkzigt to test for the presence of hepatitis B surface antigen (HBsAg)...Full Text Available

1984-10-01

157

Followup study of possible HIV seropositivity among abusers of parenteral drugs in 1971-72.

Few diseases exemplify the integration of research from bench to bedside as well as neonatal lupus, often referred to as a model of passively acquired autoimmunity. In essence, this disease encompasses...Full Text Available

2003-01-01

158

Evidence of synthesis by Lumbricus terrestris of specific substances in response to an immunization with a synthetic hapten.

Serum specimens obtained from a nationwide sample of parenteral drug abusers (PDAs) during the period 1971-72 had previously been screened for human immunodeficiency virus (HIV) antibodies. Some specimens...Full Text Available

1991-07-01

159

Evaluation of solid phase system in radioimmunoassay of triiodothyronine (Preprint No. RA.24)

Lumbricus terrestris (Annelid, Oligocheta) is capable of cellular- and humoral-specific reactions against natural antigens. Is this earthworm able to elaborate a response of antibody type against a...Full Text Available

1985-12-01

160

Establishment of an Immunoglobulin M Antibody-Forming Cell Response Model for Characterizing Immunotoxicity in Primary Human B Cells

This paper describes the evaluation of antibody coated polystyrene beads using "1"2"5I-labelled T_3 ("1"2"5I-T_3). (author).

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161

Determination of antibody response to influenza virus surface glycoproteins by kinetic enzyme-linked immunosorbent assay.

Rodent models have been extensively utilized to identify putative human immunotoxicants; however, even when immunotoxicity is established, uncertainty remains whether the effects are predictive of human...Full Text Available

2009-12-01

162

Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.

We modified an existing enzyme-linked immunosorbent assay (ELISA) to be able to use new spectrophotometers which can measure the rate of color development in microtiter wells. This new kinetic-based...Full Text Available

1988-10-01

163

Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies.

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures...Full Text Available

1991-07-01

164

Comparative Medicine - National Center for Research Resources...

The authors have recently identified a new cytokeratin (CK) polypeptide, CK 20, whose expression is almost entirely confined to the gastric and intestinal epithelium, urothelium, and Merkel cells. Seven...Full Text Available

1992-02-01

165

Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice.

and Antibody Responses of Rhesus Macaques Exposed to the Human Gammaretrovirus XMRV external link, opens in new window J Virol. 2011 May;85(9):4547-57 Detection of CWD...

2011-10-15

166

Application of an indirect immunofluorescence test for detection of Mycoplasma pneumoniae in respiratory exudates.

Plasma samples were collected from mice infected with Trypanosoma musculi at different times postinfection and administered to naive recipient mice either before or during T. musculi infection. The...Full Text Available

1985-06-01

167

Antigenic analysis of the second extra-cellular loop of the human beta-adrenergic receptors.

We prepared polyclonal antibody specific to Mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antigen in respiratory...Full Text Available

1991-09-01

168

Antibodies to synthetic peptides from the tubulin regulatory domain interact with tubulin and microtubules.

Polyclonal antibodies were raised in rabbits by immunization with free peptides corresponding to positions 197-222 of the human beta 1-adrenergic receptor (beta 1 peptide) and the corresponding sequence...Full Text Available

1989-10-01

169

An Immunomagnetic Single-Platform Image Cytometer for Cell Enumeration Based on Antibody Specificity?

The carboxyl-terminal region of tubulin alpha and beta subunits plays a major role in regulating its assembly into microtubules and constitutes an essential domain for the selective interaction of microtubule-associated...Full Text Available

1988-09-01

170

A Serological Survey of Sera from Domestic Animals on Easter Island

Simplification of cell enumeration technologies is necessary, especially for resource-poor countries, where reliable and affordable enumeration systems are greatly needed. In this paper, an immunomagnetic...Full Text Available

2007-04-01

171

Status of bismuth and lead for radioimmunoimaging and therapy

Animals' sera collected on Easter Island from December 1964 to February 1965 were tested by appropriate methods for the presence of antibodies to various infections. These included, ornithosis, Q-fever,...Full Text Available

1968-04-01

172

Immunological correlates for protection against intranasal challenge of Bacillus anthracis spores conferred by a protective antigen-based vaccine in rabbits.

Of the radionuclides with physical properties most suited for use with monoclonal antibody for tumor imaging or therapy, few are more available or desirable than Pb-203, 212 and Bi-212. Yet no clinical studies have as yet been possible solely due to the lability in vivo of known complexes. We now report that bismuth complexes of the ligands p-SCN-Bz-DOTA and p-SCN-Bz-trans-cyclohexylDTPA are effective at protecting Bi-206 from release when linked to immunoproteins. Practicable, efficient ligand syntheses will be reported. Initial studies reveal that antibody is labeled efficiently with radiolead DOTA complexes. Preliminary studies indicate stability in vivo.

1990-08-26

173

Antihuman growth hormone (GH) antibodies cross-react with the GH-like factor from plerocercoids of the tapeworm Spirometra mansonoides.

Correlates between immunological parameters and protection against Bacillus anthracis infection in animals vaccinated with protective antigen (PA)-based vaccines could provide surrogate markers to evaluate the putative protective efficiency of immunization in humans. In previous studies we demonstrated that neutralizing antibody levels serve as correlates for protection in guinea pigs (S. Reuveny et al., Infect. Immun. 69:2888-2893, 2001; H. Marcus et al., Infect. Immun. 72:3471-3477, 2004). In this study we evaluated similar correlates for protection by active and passive immunization of New Zealand White rabbits. Full immunization and partial immunization were achieved by single and multiple injections of standard and diluted doses of a PA-based vaccine. Passive immunization was carried out by injection of immune sera from rabbits vaccinated with PA-based vaccine prior to challenge with B. anthracis spores. Immunized rabbits were challenged by intranasal spore ...

2006-01-01

174

Development of radioimmunometric assays and kits for non-clinical applications. Proceedings of a final research coordination meeting

A factor produced by the plerocercoid stage of S. mansonoides mimics some, but not all, of the actions reported for hGH. The biological actions of plerocercoid growth factor (PGF) suggest structural similarity to human GH (hGH). Plerocercoid membranes were solubilized, and PGF was purified more than 1000-fold by hGH receptor affinity chromatography. The ability of purified PGF to displace [125I]hGH from monoclonal antibodies specific for four distinct nonoverlapping antigenic determinants of hGH and from an anti-hGH polyclonal antibody was tested in liquid phase RIA. All of the hGH antibodies cross-reacted with PGF, with potencies ranging from more than 60% to less than 1% that of the hGH standard. Of the four major epitopes of hGH defined by the monoclonal antibodies used in this study, only one is not represented to a significant extent in PGF. The epitope of hGH that is only marginally present in PGF ...

1987-11-01

175

Structural analysis of covalently labeled estrogen receptors by limited proteolysis and monoclonal antibody reactivity

Immunoassays are versatile analytical techniques that had a leading role in various clinical applications, during the last four decades. The studies carried out by Rosalyn Yalow, Solomon Berson and Roger Ekins in the 1960s gave a breakthrough in the development of this novel analytical method. Sensitivity up to femtomolar concentrations, high specificity and universal application to different classes of molecular species made immunoassay a very useful tool in analytical investigation. The expertise acquired by immunochemists in producing antibodies against any antigen and the ability of radiochemists in labeling the antigens with "1"2"5I without affecting the active site are the two main factors responsible for the above development. There are concerns about the safety and health of humans due to the high-level contamination of environment by pesticides, industrial compounds and metals, anabolic steroids in milk and meat products, and presence ...

2004-12-06

176

MHC-like molecules in some nonmammalian vertebrates can be detected by some cross-reactive xenoantisera.

The authors have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with ["3H]tamoxifen aziridine (["3H]TAZ) were treated with trypsin (T), #alpha#-chymotrypsin (C), or Staphylococcus aureus V8 protease prior to electrophoresis on sodium dodecyl sulfate gels. Fluorography revealed a distinctive ladder of ER fragments containing TAZ for each protease generated from the M/sub r/ 66,000 ER. Immunoblot detection with the primate-specific antibody D75P3#gamma# revealed that all immunoreactive fragments corresponded to TAZ-labeled fragments but that some small TAZ-labeled fragments were no longer immunoreactive. In contrast, use of the antibody H222SP#gamma# revealed a correspondence between TAZ-labeled ...

177

Dissociation of insulin receptor phosphorylation and stimulation of glucose transport in BC3H-1 myocytes

Rabbit antisera raised to human and chicken MHC molecules were used to immunoprecipitate cross-reactive molecules from biosynthetically and cell surface-labeled spleen and/or blood cells of representative vertebrate species. Five major points emerged: 1) There were many nonspecific cross-reactions using these techniques, so various criteria were developed to distinguish these from true MHC-like molecules. 2) Only very small subpopulations of immunogen-specific antibodies cross-reacted with MHC-like molecules in other nonmammalian species. These subpopulations were different for each species and even within a species, sometimes being so limited as to behave like alloantisera. This led to a very scattered pattern of true cross-reactions that sometimes failed to reflect the properties of the bulk antibody population. 3) Antisera containing antibodies to class II beta- and class I alpha-chains cross-reacted better and more ...

1990-01-01

178

Curious Results with Palladium- and Platinum-Carrying Polymers in Mass Cytometry Bioassays and an Unexpected Application as a Dead Cell Stain.

The authors have investigated insulin receptor phosphorylation in differentiated cultured BC3H-1 myocytes. As for other insulin-responsive cell systems in partially purified wheat germ agglutinin receptor preparations, insulin stimulates the phosphorylation of its own receptor (95K ..beta..-subunits) in a dose dependent manner (0-400 nM), as identified by immunoprecipitation with antiinsulin receptor antibodies and SDS-PAGE. In the same preparations they show that 12-0-tetradecanyl phorbol acetate (TPA), which in many respect ..beta..-subunits in the same dose dependent manner (0-5 ..mu..M). In addition, antiinsulin receptor antibodies (B-10) also induced phosphorylation of mimics insulin action, also induced phosphorylation of the insulin receptor and HPLC tryptic maps of the /sup 32/P-labeled ..beta..-subunit were identical to those for insulin-induced receptor phosphorylation. However, while insulin and TPA are potent stimulators of glucose ...

1986-05-01

179

No evidence of over-reporting of SARS in mainland China

We describe the synthesis of metal-chelating polymers (MCPs) with four different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, either a fluorescein molecule or a bismaleimide linker for antibody attachment. Polymer characterization by a combination of (1)H NMR, UV/vis absorption measurements, and thermal gravimetric analysis (TGA) indicated that each chain of the fluorescein-terminated polymers contained one dye molecule. These polymer samples were loaded with three different types of lanthanide ions as well as palladium and platinum ions. The numbers of metal atoms per chain were determined by a combination of UV/vis and conventional ICP-MS measurements. The experiments with lanthanide ions demonstrated that a net anionic charge on the polymer is important for water solubility. These experiments also showed that at least one type of lanthanide ion (La(3+)) is capable of forming a bimetallic complex with pendant DTPA ...

2011-09-28

180

British Library Electronic Table of Contents (United Kingdom)

Summary Objective To find out whether there was over-reporting of SARS patients in mainland China in view of the relatively low case fatality ratio in mainland China, in comparison with other affected countries and areas. Methods We searched PubMed for all SARS antibody detection papers (in English or Chinese language) using the keywords `SARS' and `antibody'. Then the resulting articles were further read through to select the SARS detection results using ELISA methods of serum samples collected at least 1 month after disease onset. A multi-level logistic regression was applied to test for possible differences in the proportions positive between locations of the study. Results A total of 48 studies were identified, including 39 from mainland China and nine from elsewhere (Hong Kong, Taiwan...

2009-01-01

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181

Mesenchymal stem cell transplantation for diffuse alveolar hemorrhage in SLE

British Library Electronic Table of Contents (United Kingdom)

Background. A 19-year-old girl was diagnosed with systemic lupus erythematosus, based on findings of arthritis, malar rash, positive antinuclear antibody test and high levels of antibodies to double-stranded DNA. Two months after diagnosis, the patient presented with a sudden drop in blood hemoglobin level. Several days later, she developed bloody sputum, rapidly progressive dyspnea and hypoxemia. High-resolution CT showed diffuse alveolar infiltrates in both lung fields.Investigations. Physical examination, complete blood count, erythrocyte sedimentation rate, urinalysis, 24-h urine protein excretion, fecal occult blood test, d-dimer test, acid hemolysis test, activated partial thromboplastin time and prothrombin time, direct and indirect Coombs tests, bone marrow smear, arterial blood ga...

2010-01-01

182

In vivo chicken model for peripheral intravascular human fibrin clot detection

Generation and Characterization of Monoclonal Antibodies to Human Keratinocyte Growth Factor Receptor

A chicken model was prepared that provides a simple and economical method of evaluating the use of fibrin-specific monoclonal antibody 64C5 in the detection of peripheral vascular thrombi. Human fibrin was clotted in segments of a chicken's femoral artery and vein prior to intravenous injection of radioiodinated antibody 64C5. After a 3-hr perfusion time, the thrombosed and contralateral control segments of the vessels were excised and counted for radioactivity. The radiolabeled 64C5 uptake ratio of the thrombosed segment to the control segment was 5.4 +/- 1.2 (p less than 0.007) in the femoral artery, and 3.8 +/- 1.1 (p less than 0.02) in the femoral vein. This in vivo chicken model may also find application in studies of targeting agents for human fibrin.

1988-08-01

183

British Library Electronic Table of Contents (United Kingdom)

Keratinocyte growth factor receptor (KGFR) and fibroblast growth factor receptor (FGFR) 2c share identical amino acid sequences, except for a 46-amino acid domain in the extracellular region. Monoclonal antibodies (MAbs) specific to KGFR have not been reported nor are commercially available. In this study, we generated murine MAbs specific to KGFR in non-obese diabetic (NOD) mice using a modified Repeated Immunizations at Multiple Sites (RIMMS) technology. Stable cell lines expressing the full-length human KGFR or FGFR2c were produced to facilitate the identification of KGFR-specific MAbs. Following the initial screening of hybridoma clones with a fluorescence-based, confocal cell detection method and ELISA, KGFR-specific MAbs were selected and confirmed by flow cytometry and Western blot ...

2006-01-01

184

Follow-up of pregnant women exposed to chicken pox: an audit of relationship between level of antibody and development of chicken pox

British Library Electronic Table of Contents (United Kingdom)

The purpose of this study was to validate through natural exposure a cut-off level of varicella zoster IgG as protective against infection with varicella zoster virus (VZV). Laboratory testing to determine VZV immune status of pregnant women exposed to varicella is recommended. Quantitative assays are now available which are sensitive and specific. More than 200 consecutive requests for screening in pregnant patients with recent varicella contacts were followed-up by questionnaire. DiaSorin LIAISON and VZV time resolved fluorescence immuno assay (VZV TRFIA) were used to measure VZV antibody level. One hundred fifty out of 209 (72%) questionnaires were returned; 14 patients developed varicella, 129 did not and seven were not known. Patients who had been given VZIG and developed varicella on...

2011-01-01

185

Antigenic characterization of phocine distemper virus causing mass mortality in 2002 and its relationship to other morbilliviruses

British Library Electronic Table of Contents (United Kingdom)

Summary The antigenic relationship between the phocine distemper virus (PDV) strain causing the epidemic in 2002 and the PDV strain of 1988, canine distemper virus from two dogs and one marten, and one measles virus strain was investigated in vivo and in vitro using monospecific polyclonal and monoclonal antibodies directed against five different proteins of canine or phocine distemper virus (N, P, M, F, H). Epitopic mapping revealed no difference between the PDV strains causing the epidemics in 1988 or 2002. However, the use of these antibodies allowed discrimination between different morbilliviruses including a vaccine strain of canine distemper virus. The major differences among the investigated morbilliviruses were found in the H protein.

2007-01-01

186

Adalimumab treatment in Crohn's disease does not induce early changes in regulatory T cells

British Library Electronic Table of Contents (United Kingdom)

Abstract Objective. Anti-TNF-a antibodies has been suggested to modulate regulatory T cell (Treg) percentages in rheumatoid arthritis, but results from studies of Crohn's disease (CD) are conflicting. We investigated dynamic changes of circulating Tregs in CD during treatment with the anti-TNF-a-antibody adalimumab (Humira, Abbott Laboratories A/S, Emdrupvej 28C, DK-2100 Copenhagen). Material and methods. Blood samples from 26 CD patients were analysed using flow cytometry before and 1 and 26 weeks after initiation of adalimumab treatment to determine the percentage of Tregs among CD4++ T cells. Results. In spite of a significant decline in disease activity scores and biochemical markers of inflammation, during the first week of treatment, we did not observe early modulating effects of ada...

2011-01-01

187

Cyclotron Production of Radioisotopes

The incidence of spontaneously occurring antiliver antibodies in New Zealand Black mice

Production of Radioactive Isotopes in Republic of Kasakhstan.

188

The EGFR antibody cetuximab induces autophagy in cancer cells by downregulating HIF-1? and Bcl-2 and activating the beclin-1/hVps34 complex

The sera of NZB/BL mice have been found to be active in the complement fixation test against isogeneic liver antigens to a higher titre than the sera of CBA, C57BL and DBA/2 mice. Six sera from NZB...Full Text Available

1969-04-01

189

Staphylococcus aureus Enterotoxin D Is Secreted in Milk and Stimulates Specific Antibody Responses in Cows in the Course of Experimental Intramammary Infection

Autophagy is a regulated catabolic process triggered in cells deprived of nutrients or growth factors that govern nutrient uptake. Here we report that autophagy is induced by cetuximab, a therapeutic...Full Text Available

2010-07-15

190

Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects.

An enterotoxin D (SED)-producing strain of Staphylococcus aureus was used to infect one mammary gland of each of 17 lactating dairy cows. All glands became infected and shed bacteria...Full Text Available

2006-06-01

191

Novel method for differentiation between Trastuzumab and host adaptive response.

The IgG response to gp41 (envelope glycoprotein of Mr 41,000) of the human immunodeficiency virus (HIV) was studied with eight synthetic peptides derived from three different regions of the protein....Full Text Available

1988-07-01

192

Murine antibody response to oral infection with live aroA recombinant Salmonella dublin vaccine strains expressing filamentous hemagglutinin antigen from Bordetella pertussis.

Humoral immune response to human epidermal growth factor receptor 2 (HER-2/neu or ErbB-2) has been detected in sera of breast cancer patients and shown to be an appropriate prognostic marker (Taylor et al., 2007). However, since Trastuzumab (Herceptin) is a widely used monoclonal antibody as cancer therapy agent for tumors over-expressing HER-2, there is a need for an efficient way to detect host-generated antibodies against HER-2 without the confounding effect of Herceptin. Here we describe a screening method developed to decipher between host antibodies against HER-2 and that of Herceptin. By producing a series of truncation mutants within the epitope of Herceptin, we were able to inhibit this binding. We demonstrated also that by a three amino acid substitution (PPF?SSS) we were able to abrogate Herceptin binding while generating a highly conserved HER-2 extracellular domain (ECD). By producing a stable cell line that ...

2011-06-08

193

Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice.

Two plasmids which express either nearly intact or truncated filamentous hemagglutinin (FHA) from Bordetella pertussis and which are marked with a tetracycline resistance (Tcr) gene were transformed into Salmonella dublin SL1438, an aroA deletion mutant intended for use as an attenuated oral vaccine against salmonellosis. These S. dublin recombinants, when fed to mice, induced serum immunoglobulin, immunoglobulin M (IgM), and sometimes IgA antibody responses to FHA and S. dublin. In addition, IgA antibodies against FHA were found in gut wash fluids. S. dublin carrying pDB2300, a multicopy plasmid encoding truncated FHA protein, induced a better antibody response than did S. dublin carrying pDB2000, a low-copy-number plasmid encoding full-sized FHA. Administration of tetracycline to mice enhanced the stability of recombinant plasmids, and tetracycline-treated mice developed higher anti-FHA titers. Although neither strain ...

1990-08-01

194

Monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60.

We have recently shown that viable Borrelia burgdorferi organisms induce a chronic infection associated with arthritis and carditis in severe combined immunodeficiency (scid) mice but not in immunocompetent...Full Text Available

1990-05-01

195

Lyme myocarditis diagnosed by indium-111-antimyosin antibody scintigraphy

Ocular and urogenital tract infections with Chlamydia trachomatis can progress to chronic inflammatory diseases that produce blindness and tubal infertility. The pathophysiology of these chronic disease...Full Text Available

1992-06-01

196

Kidney-specific allo- and autoantibodies in the alloantibody response to rat kidney: the use of kidney homogenate as a target for serological analysis.

We report a new case of Lyme disease with cardiac manifestations, which has been possible to follow during the long period of 12 years. We have detected the usual ECG abnormalities, and concentric hypertrophic myocardiopathy, by echocardiography. The acute myocarditis was demonstrated by /sup 111/In-antimyosin scintigraphy, which showed global myocardial uptake of the tracer, constituting the first report, to our knowledge, of Lyme myocarditis diagnosed by this method.

1989-06-01

197

Induction of Antibody Responses to African Horse Sickness Virus (AHSV) in Ponies after Vaccination with Recombinant Modified Vaccinia Ankara (MVA)

LEW anti-DA kidney and DA anti-LEW kidney sera were assayed using an indirect 125I anti-immunoglobulin-binding assay with kidney homogenate as target. This allowed the full spectrum of antibodies to...Full Text Available

1980-04-01

198

Glutamine-linked and Non-consensus Asparagine-linked Oligosaccharides Present in Human Recombinant Antibodies Define Novel Protein Glycosylation Motifs

BackgroundAfrican horse sickness virus (AHSV) causes a non-contagious, infectious disease in equids, with mortality rates that can exceed 90% in susceptible horse populations....Full Text Available

199

False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV

We report the presence of oligosaccharide structures on a glutamine residue present in the V_L domain sequence of a recombinant human IgG2 molecule. Residue Gln-106, present in the QGT sequence...Full Text Available

2010-05-21

200

Expression of a functional VEGFR-1 in tumor cells is a major determinant of anti-PlGF antibodies efficacy

To evaluate the reactivity of the recombinant proteins expressed in Escherichia coli strain BL21(DE3), a Western blot assay was performed by using a panel of 78 serum samples obtained,...Full Text Available

2006-03-01

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201

Enzymic synthesis of 1-O-(indol-3-ylacetyl)-beta-D-glucose. Purification of the enzyme from Zea mays, and preparation of antibodies to the enzyme.

PlGF, one of the ligands for VEGFR-1, has been implicated in tumor angiogenesis. However, more recent studies indicate that genetic or pharmacological inhibition of PlGF signaling does not result in...Full Text Available

2011-07-12

202

Diversion of the Immune Response to Neisseria gonorrhoeae from Th17 to Th1/Th2 by Treatment with Anti-Transforming Growth Factor ? Antibody Generates Immunological Memory and Protective Immunity

The enzyme indol-3-ylacetylglucose synthase (UDP-glucose:indol-3-ylacetate beta-D-glucosyltransferase) catalyses the reaction: [formula: see text] This is the first step in the series of reactions leading...Full Text Available

1991-10-15

203

Differential Specificity and Immunogenicity of Adenovirus Type 5 Neutralizing Antibodies Elicited by Natural Infection or Immunization?

ABSTRACTThe immune response to Neisseria gonorrhoeae is poorly understood, but its extensive antigenic variability and resistance to complement are thought to allow it...Full Text Available

204

Developmentally regulated expression of a 78 kDa erythroblast membrane glycoprotein immunologically related to the platelet thrombospondin receptor.

A recent clinical trial of a T-cell-based AIDS vaccine delivered with recombinant adenovirus type 5 (rAd5) vectors showed no efficacy in lowering viral load and was associated with increased risk of...Full Text Available

2010-01-01

205

Development of an assay for a biomarker of pregnancy and early fetal loss

We have previously described a monoclonal antibody (FA6-152), obtained by immunizing mice with fetal human erythrocytes [Edelman, Vinci, Villeval, Vainchenker, Henri, Miglierina, Rouger, Reviron, Breton-Gorius,...Full Text Available

1989-09-15

206

Commercially Available Antibodies to Human Tumour Necrosis Factor-? Tested for Cross-Reactivity with Ovine and Bovine Tumour Necrosis Factor-? using Flow Cytometric Assays

Human chorionic gonadotropin (hCG) is a glycoprotein hormone, secreted by the syncytiotrophoblast cells of the fertilized ovum, that enters the maternal circulation at the time of endometrial implantation. It is composed of two nonidentical subunits; ..cap alpha.. and ..beta.., with molecular weights of 14 kD and 23 kD, respectively. Human chorionic gonadotropin binds to the same receptor as hLH and displays the same biological response, namely, to stimulate the declining function of the corpus luteum to produce progestins and estrogen late in the menstrual cycle. The differences in the structures of hCG and hLH have been exploited to develop antibodies that can measure hCG specifically in the presence of hLH. Two-site antibody binding assays have been developed, based on a surface immunological concept of hCG epitopes, that involve four distinct regions to which antibodies against hCG can bind simultaneously. ...

1987-10-01

207

A Single Domain Llama Antibody Potently Inhibits the Enzymatic Activity of Botulinum Neurotoxin by Binding to the Non-Catalytic Alpha-Exosite Binding Region

A thorough understanding of the immune system, including the role of different cytokines, during inflammatory diseases in ruminants could lead to the development of new diagnostic methods and treatments....Full Text Available

2004-01-01

208

??⁺ T-Lymphocyte Cytotoxicity against Envelope-Expressing Target Cells Is Unique to the Alymphocytic State of Bovine Leukemia Virus Infection in the Natural Host

Ingestion or inhalation of botulinum neurotoxin (BoNT) results in botulism, a severe and frequently fatal disease. Current treatments rely on antitoxins, which while effective cannot reverse...Full Text Available

2010-04-09

209

Effect of inhaled beclomethasone dipropionate on isocapnic hyperventilation with cold air in asthmatics, measured with forced oscillation technique.

Bovine leukemia virus (BLV) is a complex B-lymphotrophic retrovirus of cattle and the causative agent of enzootic bovine leukosis. Serum antibody in infected animals does not correlate with protection...Full Text Available

2000-09-01

210

Isocapnic hyperventilation with cold air (IHCA) is a reliable technique for assessing indirect bronchial hyperresponsiveness in patients with asthma. Impedance measurement of the respiratory system by the forced pseudorandom noise oscillation technique is a sensitive technique to assess changes in bronchial tone after IHCA. The aim of this study was to evaluate the effect of 6 weeks of treatment with beclomethasone dipropionate, 1,000 microg x day-1, on IHCA in asthmatic patients, measured with both forced oscillation technique and flow-volume recordings. Forty patients with mild asthma were included in this double-blind, placebo-controlled parallel-group study. Stratification on the basis of sex was performed to overcome differences in airway diameter. At entry and every 2 weeks during the treatment period, IHCA was performed and patient diaries were evaluated. Characteristic changes in forced oscillation parameters after IHCA were observed in all patients. After 6 weeks of treatment, ...

1997-03-01

211

Subcellular distribution of ryanodine receptors in the cardiac muscle of carp (Cyprinus carpio).

... Radiation Protection Products and Equipment Find and compare a variety of radiation protection products and equipment on the world's largest environmental industry portal. View product ...

212

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin

We examined the subcellular localization of ryanodine receptors (RyR) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods and compared it with those of rats and guinea pigs. To achieve this goal, an anti-RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs. Western blot analysis using this antibody detected a single RyR band following the SDS-PAGE of sarcoplasmic reticulum (SR) membranes from carp atrium and ventricle as well as from mammalian hearts and skeletal muscles. The carp heart band had slightly greater mobility than those of mammalian hearts. Although immunohistochemical staining showed evident striations corresponding to the Z lines in longitudinal sections of mammalian hearts, clusters of punctate staining, in contrast, were distributed ubiquitously throughout carp atrium and ventricle. Electron ...

2003-06-12

213

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin

Previous work showed that (TH)p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the ...

1985-08-25

214

In vivo evaluation of a lead-labeled monoclonal antibody using the DOTA ligand

Previous work showed that ["3H]p-azidopuromycin photoaffinity labeled 70 S Escherichia coli ribosomes and that photoincorporation into 50 S subunit proteins was in the order L23 greater than L18/22 greater than L15. In the present work the authors report on immunoelectron microscopic studies of the complexes formed by p-azidopuromycin-modified 50 S subunits with antibodies to the N6,N6-dimethyladenosine moiety of the antibiotic. The p-azidopuromycin-modified 50 S subunits appear to be identical to unmodified control subunits in electron micrographs. Complexes of modified subunits with antibodies to the N6,N6-dimethyladenosine moiety of p-azidopuromycin were visualized in micrographs. Two regions of p-azidopuromycin photoincorporation were identified. The primary site, seen in about 75% of the complexes, is between the central protuberance and small projection, on the side away from the L7/L12 arm, in a region thought to contain the ...

215

Enzyme-linked immunosorbent assays for alpha-synuclein with species and multimeric state specificities.

The aim of this study was to assess the utility of a radioimmunoconjugate containing a lead radionuclide for therapy and scintigraphy applications. The radioimmunoconjugate evaluated consisted of a bifunctional DOTA ligand and monoclonal antibody (MAb) B72.3 using athymic mice bearing LS-174T tumors, human colon carcinoma xenografts. In the studies reported here, the lead-203-DOTA complex itself was first demonstrated to have in vivo stability. MAb B72.3 was then conjugated with the DOTA ligand and labeled with "2"0"3Pb, and the immunoreactivity of B72.3 was maintained. The localization of the radioimmunoconjugate to tumor tissue and other select organs paralleled that of DOTA-"1"2"5I-B72.3, suggesting a similar metabolic pattern of the two radioimmunoconjugates. Thus, the DOTA-metal complex does not alter the behavior of the radioimmunoconjugate. Tumor localization of the "2"0"3Pb-DOTA-B72.3 conjugate was demonstrated with biodistribution studies as well as ...

1998-05-01

216

Distribution of Ehrlichia canis among military workingn> dogs in the world and selected civilian dogs in the United States.

Abnormal intracellular deposition of aggregated ?-synuclein is the characteristic feature of a number of neurological disorders, including Parkinson's disease (PD). Although ?-synuclein is typically known as a cytosolic protein, a small amount is secreted by exocytosis in both monomeric and aggregated forms. The extracellular forms of ?-synuclein in human body fluids, such as cerebrospinal fluid (CSF) and blood plasma, might be a diagnostic target for PD and related diseases. Here, we characterized a new set of monoclonal antibodies against ?-synuclein, and using different combinations of antibodies, we established ELISA systems to specifically detect human ?-synuclein, mouse and human ?-synuclein together, and multimeric forms of ?-synuclein in biological samples. By employing the Tyramide signal amplification method, the sensitivity of the assay was significantly improved to detect a concentration as low as ?12.5 pg/ml. These assays might be ...

2011-05-31

217

Anti-nuclear antibody positivity and the use of certolizumab in inflammatory bowel disease patients who have had arthralgias or lupus-like reactions from infliximab or adalimumab

Antibodies to Ehrlichia canis were detected by indirect immunofluorescence in sera from 233 of 2,077 (11%) military working dogs in various locations throughout the world and from 535 of 938 (57%) civilian dogs in the United States during a 1-year period of study. Overall, E canis infection rates ranged from 13% in the tropical and temperate zones below 45 degrees N to 8% in the cold zone north of 45 degrees N latitude. The highest antibody prevalence rate (24%) was found among a select population of dogs stationed between 40 degrees and 45 degrees north latitude (Japan and Okinawa). The seropositive military dogs did not have clinical signs of ehrlichiosis, thus indicating that the predominant form of infection was subclinical. On the other hand, 216 (23%) of the seropositive civilian dogs had various signs of the disease. The difference was attributed to the fact that the sera from civilian dogs were submitted by practitioners who suspected ...

1982-08-01

218

British Library Electronic Table of Contents (United Kingdom)

OBJECTIVE:- Tumor necrosis factor (TNF) inhibitors can be used to treat inflammatory bowel disease (IBD) but may lead to anti-nuclear antibody (ANA) positivity and lupus-like reactions. Because of its unique structure, certolizumab has lower rates of these complications. We sought to investigate whether patients who have had lupus-like reactions to infliximab or adalimumab would be able to tolerate certolizumab. METHODS:- We performed a retrospective analysis on the 23 patients at the Roberts Inflammatory Bowel Disease Center who received certolizumab for the treatment of Crohn's disease from March 2008 to June 2009. We identified 6 patients who were switched to this drug because of lupus-like reactions from prior anti-TNF therapy and had documented ANA after the reaction and prior to cert...

2011-01-01

219

A CD33-specific single-chain immunotoxin mediates potent apoptosis of cultured human myeloid leukaemia cells.

{sigma}-Hyperon production at COSY-TOF

A novel single-chain immunotoxin was constructed by combining a CD33-specific single chain Fv (scFv) antibody fragment with an engineered variant of Pseudomonas exotoxin A (ETA). The variant toxin carries the KDEL peptide at its C-terminus, a cellular peptide mediating improved retrograde transport to the endoplasmic reticulum. The purified recombinant fusion protein induced potent apoptosis of the human myeloid cell lines U937, HL-60 and THP-1. Up to 98% of U937 cells were eliminated after treatment for 72 h with a single dose of 500 ng/ml (c. 7 nmol/l). Killing was antigen-specific and occurred by apoptosis. A control protein, consisting of a CD19-specific scFv antibody fragment fused to the ETA-KDEL toxin, failed to induce death of the CD19-negative cell lines U937, HL-60 and THP-1. The CD33-ETA toxin also mediated apoptosis of fresh patient-derived acute myeloid leukaemia cells from bone marrow and peripheral blood. The pronounced ...

2006-04-01

220

{lambda}-Hyperon production at COSY-TOF

No abstract prepared.

2002-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

Universality in production processes

No abstract prepared.

2002-03-01

222

Production of {omega}-mesons at COSY-11

Short communication. 1 ref.

1996-12-31

223

OXIDE SUPERCONDUCTOR AND ITS PRODUCTION

No abstract prepared.

2002-03-01

224

J-STORE (Japan)

Full Text Available

2007-12-14

225

Purification of cyclotron-produced {sup 203}Pb for labeling Herceptin

Pretargeted radioimmunotherapy in tumored mice using an in vivo {sup 212}Pb/{sup 212}Bi generator

A simple and rapid procedure was developed for the purification of cyclotron-produced {sup 203}Pb via the {sup 203}Tl(d,2n) {sup 203}Pb reaction. A Pb(II) selective ion-exchange resin, with commercial name Pb Resin from Eichrom Technologies, Inc., was used to purify {sup 203}Pb from the cyclotron-irradiated Tl target with excellent recovery of the enriched Tl target material. The purified {sup 203}Pb was used to radiolabel the monoclonal antibody Herceptin. The in vitro and in vivo properties of the {sup 203}Pb radioimmunoconjugate were evaluated.

2005-04-01

226

Multicellular level dosimetry and low dose rate effects

Objective: Pretargeting is the concept that combines optimal delivery of the antibody and rapid capture and elimination of the radioactivity. In this study, we evaluated the potential of antibody pretargeting to enable the tumor-targeting {sup 212}Pb for in vivo generation of {sup 212}Bi for {alpha} particle radiotherapy. Methods: The {sup 212}Pb/{sup 212}Bi chelate of DOTA-biotin, as well as their {gamma}-emitting analogues, {sup 203}Pb and {sup 205}Bi, was prepared and characterized. The radiolabeled compounds were injected in animals for evaluation of tumor targeting and normal tissue uptake and retention. In the pretargeting protocol, injection of 400 {mu}g of NR-LU-10 antibody-streptavidin conjugate was given at t=0 h, then 100 {mu}g of N-acetyl-galatosamine-biotin clearing agent was injected at t=20-24 h; finally, 1 {mu}g of {sup 212}Pb/{sup 212}Bi-DOTA-biotin was injected 6 h later. Results: Both {sup 203}Pb and {sup ...

2005-10-01

227

Labeled cells as research, diagnostic and therapeutic tools

Building on the concepts of MIRD methods described by Webber, Watson and others at this Symposium on the Dosimetry of Administered Radionuclides, we will now consider how to apply these principles to several experimentally based open-quote macroclose quotes and multicellular models. Specifically, methods to calculate absorbed dose using different types of particulate emission (alpha, beta) with a variety of antibody carriers (IgG, F(ab')_2, Fab) for radioimmunotherapy (RIT) will be reviewed. Additionally, a discussion of direct measurement methods at the multicellular level will reveal the problems of tumor absorbed dose heterogeneity when applied to animal and clinical studies.

1989-09-21

228

Cutaneous leishmaniasis associated with jungle training.

Scientists at JPL have developed chemical and biological techniques using microspheres filled with drugs, electron-opaque metals, or radioactive, fluorescent, magnetic or electrically charged materials to label specific groups of cells. Synthetic polymeric microspheres are coupled with specific antibodies to form reagents called immunomicrospheres, which can seek out and attach themselves to any specific group of cells. These cell-labeling techniques, therefore, open new avenues not only to the basic study of cells but also to the diagnosis and treatment of many diseases, including cancer.

1983-09-01

229

Cutaneous gallium uptake in patients with AIDS with mycobacterium avium-intracellulare septicemia

In November 1977, 627 soldiers belonging primarily to the First Battalion, 82nd Airborne Division, stationed at Fort Bragg, were sent to the Canal Zone, Panama, for jungle warfare training. A medical surveillance program incorporating pre- and post-evaluations over a 6-month period with dermatologic examinations, questionnaires, and serologic tests was established. Ten cases of cutaneous leishmaniasis (1.6/100 men) were diagnosed by positive Leishmania culture. The demonstrated lack of sensitivity and specificity of the indirect fluorescent antibody test and the direct agglutination test render these serological methods useless as diagnostic screening methods in the early stages of this disease. PMID:7406101

1980-07-01

230

In vivo studies of the long-term "5"1Cr red cell survival of serologically incompatible red cell units

Gallium imaging is increasingly being used for the early detection of complications in patients with AIDS. A 26-year-old homosexual man who was HIV antibody positive underwent gallium imaging for investigation of possible Pneumocystis carinii pneumonia. Widespread cutaneous focal uptake was seen, which was subsequently shown to be due to mycobacterium avium-intracellulare (MAI) septicemia. This case demonstrates the importance of whole body imaging rather than imaging target areas only, the utility of gallium imaging in aiding the early detection of clinically unsuspected disease, and shows a new pattern of gallium uptake in disseminated MAI infection.

231

Autoradiographic analysis of radiolabeled anti-carcinoembryonic antigen monoclonal antibody CEA 102 in colorectal cancer using computed radiography

The long-term survival of serologically incompatible red cell units was measured in five patients with antibodies to high-frequency antigens. Initially, the survival of 1 ml of "5"1Cr-labeled incompatible red cells was measured over 1 hour. After demonstrating that the 1-hour survival times were successful (greater than 70%), each patient then received 5 ml of the same "5"1Cr-labeled red cells followed by the transfusion of the remainder of the red cell unit. The long-term T 1/2Cr survival for each case was patient 1 (anti-McCa), 15 days; patient 2 (anti-JMH), 12 days; patient 3 (anti-Kna), 31 days; patient 4 (anti-McCa), 12 days; and patient 5 (anti-Hya), 14 days. Each antibody tested in an in vitro homologous macrophage assay showed less than 5 percent phagocytosis. Anti-JMH was the only antibody to react with IgG subclass antisera and was determined to be IgG4. The macrophage assay, IgG subclass testing, and short-term ...

232

Autoradiographic analysis of radiolabeled anti-carcinoembryonic antigen monoclonal antibody CEA 102 in colorectal cancer using computed radiography

Anti-carcinoembryonic antigen monclonal antibody (MAb) CEA 102 was produced by immunization with purified CEA and the specific accumulation of radiolabeled CEA 102 in colorectal cancers was investigated by autoradiography of sugical specimens using Fuji Computed Radiography (FCR). Five patients with colorectal cancer were injected intravenously with {sup 131}I-labeled intact CEA 102 or its F(ab'){sub 2}. Primary tumor and liver metastases were successfully detected by external scanning with a gamma camera in 4 cases. Autoradiographic study of the surgical specimens using FCR showed predominant localization of {sup 131}I-labeled CEA 102 in primary tumors and liver metastases in all cases. Even a small liver metastasis (0.5 cm) was clearly visualized in the autoradiogram by FCR. The pixel distribution curves of the density of the respective tissues in the autoradiograms by FCR showed the heterogeneity of the distribution of administreted radiolabeled MAb in ...

1992-04-01

233

Anti-carcinoembryonic antigen monclonal antibody (MAb) CEA 102 was produced by immunization with purified CEA and the specific accumulation of radiolabeled CEA 102 in colorectal cancers was investigated by autoradiography of sugical specimens using Fuji Computed Radiography (FCR). Five patients with colorectal cancer were injected intravenously with "1"3"1I-labeled intact CEA 102 or its F(ab')_2. Primary tumor and liver metastases were successfully detected by external scanning with a gamma camera in 4 cases. Autoradiographic study of the surgical specimens using FCR showed predominant localization of "1"3"1I-labeled CEA 102 in primary tumors and liver metastases in all cases. Even a small liver metastasis (0.5 cm) was clearly visualized in the autoradiogram by FCR. The pixel distribution curves of the density of the respective tissues in the autoradiograms by FCR showed the heterogeneity of the distribution of administreted radiolabeled MAb in individual tumors, ...

1992-01-01

234

Rubomycine Production

Processes for UO_2 production

Development of Experimental Large-scale Technology and Creation of Technological Line of Rubomicine Production

235

... Figure 4. Production Flowsheets based on Purification of Yellow Cake

1975-09-08

236

PRODUCT NAME BARITE

Lithography Production Techniques for next Generations of Microelectronics

the low levels of crystalline silica in this product, chronic health effects are not anticipated with normal

237

Information technology and the productivity paradox assessing the value of investing in IT

Lithography Production Techniques for next Generations of Microelectronics

1992-01-01

238

High Purity Beryllium Production

Information technology and the productivity paradox

1999-01-01

239

From Products to Services Insights and Experience from Companies Which Have Embraced the Service Economy

Production of High-Purity (>99.99% wt.) Condensed Beryllium

240

Combustion of Crop Production Wastes

From Products to Services

2008-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Centrifugal Extractors for Medicine Production

Development, Investigation and Improvement of a System of Incinerating Crop Production Wastes in Small Capacity Boilers

242

Boron-10 Materials Production

Continuous Countercurrent Process in Cascade of Centrifugal Extractors for Medicine Production

243

Alternative Technologies for Medical Radionuclide Production

Development of Technologies for Boron-10 Isotope Modified Boron-containing Materials Production and their Properties Investigation.

244

Poultry Production | Ag 101 | Agriculture | US EPA

Creation of Economic Alternative Technologies for Radionuclide Production for Medical Diagnostics and Therapy

245

... Poultry Production | Ag 101 | Agriculture | US EPA Ag 101 provides a brief overview of poultry production in the U.S.... Poultry Production Jump to main content. Ag 101 Contact Us Search: All EPA This Area You are here: EPA Home Agriculture ...Ag 101 Poultry Production Poultry Production Poultry production is an important and diverse component of American agriculture. Poultry products including eggs, chicken and turkey meat are ... In 1997, nearly 99,700 farms were producing poultry and poultry products (egg, broiler, and turkey; NASS/USDA). While broiler chicken ...

246

Automated NDT for large diameter tubular products

... Waste Collection Systems Products and Equipment Find and compare a variety of waste collection systems products and equipment on the world's largest environmental industry portal. View product ...

247

Multi-modality treatment of primary nonresectable intrahepatic cholangiocarcinoma with "1"3"1I anti-CEA--a Radiation Therapy Oncology Group Study

Ultrasonic and eddy-current techniques are used to automatically examine large diameter tubular products during their production for defective areas and out-of-tolerance conditions.

1976-09-06

248

Epitope mapping and functional analysis of sigma A and sigma NS proteins of avian reovirus

Thirty-seven patients with primary nonresectable intrahepatic cholangiocarcinoma (57% with prior treatment and/or metastasis) were prospectively treated with external radiation, chemotherapy, and "1"3"1I labelled anti-CEA. Therapy began in all trials with whole liver irradiation (21.0 Gy, 3.0 Gy/Fx, 4 days/week, 10 MV photons) with alternate treatment day chemotherapy (Adriamycin, 15 mg + 5-FU, 500 mg). One month after external beam therapy, chemotherapy was given (Adriamycin, 15 mg + 5-FU, 500 mg) followed the next day by the first administration of "1"3"1I anti-CEA. The treatment schedule used was 20 mCi day 0; 10 mCi day 5 as an outpatient. This schedule was derived from tumor dose estimates which indicated that 20 mCi (8-10 mCi/mg IgG) was sufficient to achieve tumor saturation with a tumor effective half-life of 3 to 5 days, depending upon the species of animal from which the antibody was obtained. The median tumor dose for the 20 mCi + 10 mCi regimen was 6.2 ...

249

The health impact of pemphigus vulgaris and pemphigus foliaceus assessed using the Medical Outcomes Study 36-item short form health survey questionnaire

We have previously shown that avian reovirus (ARV) #sigma#A and #sigma#NS proteins possess dsRNA and ssRNA binding activity and suggested that there are two epitopes on #sigma#A (I and II) and three epitopes (A, B, and C) on #sigma#NS. To further define the location of epitopes on #sigma#A and #sigma#NS proteins and to further elucidate the biological functions of these epitopes by using monoclonal antibodies (MAbs) 62, 1F9, H1E1, and 4A123 against the ARV S1133 strain, the full-length and deletion fragments of S2 and S4 genes of ARV generated by polymerase chain reaction (PCR) were cloned into pET32 expression vectors and the fusion proteins were overexpressed in Escherichia coli BL21 strain. Epitope mapping using MAbs and E. coli-expressed deletion fragments of #sigma#A and #sigma#NS of the ARV S1133 strain, synthetic peptides, and the cross reactivity of MAbs to heterologous ARV strains demonstrated that epitope II on #sigma#A was located at amino acid residues ...

2005-02-20

250

British Library Electronic Table of Contents (United Kingdom)

Summary Background Pemphigus vulgaris and pemphigus foliaceus are rare, potentially life-threatening, autoimmune disorders characterized by antibodies to epidermal adhesion molecules. Clinical characteristics are painful chronic erosions of mucous membranes and of the skin. There are only few published studies on the impact of the disease on the health status (HS) of patients with these conditions. Objectives To assess the impact of disease on the HS of patients with pemphigus. Methods Fifty-eight patients enrolled at the Bullous Skin Diseases Unit of IDI-IRCCS in the period January-June 2006 were assessed for their HS using the Medical Outcomes Study 36-item short form health survey (SF-36) questionnaire and for anxiety and depression using the Institute for Personality and Ability Testin...

2008-01-01

251

Serological testing-An alternative to boosters?

British Library Electronic Table of Contents (United Kingdom)

The issue of the duration of immunity, particularly for the modified live viral components of veterinary vaccines, has been a significant part of the recent vaccination debate. One manufacturer has increased the recommended booster interval for these components to 3 years give name and another now states `up to 4 years' immunity.There remain many unanswered questions regarding this duration of immunity (DOI). Studies suitable for data sheet claims are time consuming and costly and can only be performed in laboratory dogs under tightly controlled conditions. Evidence from rabies serology testing in the UK shows that the response of individual animals to routine vaccination is highly variable. Much of the published field evidence on the persistence of antibody titres originates from North Am...

2006-01-01

252

Serological Reactivity to Borrelia burgdorferi Sensu Lato in Dogs and Horses from Distinct Areas in Romania

British Library Electronic Table of Contents (United Kingdom)

Abstract Lyme disease is a perfect model of the complex relationship between host, vector, and the vector-borne bacteria. Both dogs and horses in Romania are exposed to infection. The aim of the present study was to assess the seroreactivity against Borrelia burgdorferi sensu lato in dogs and horses from different regions of Romania. 276 samples from dogs and 260 samples from horses located in different regions of Romania were analyzed by ELISA and IFA, respectively. The effect of several factors potentially affecting seroreactivity (location, age, gender, occupation, and vector exposition risk) was evaluated using Fisher's exact test (R 2.12.0). The overall prevalence of anti-Borrelia antibodies was 6.52% (18/276) in dogs, with a significantly higher positivity (46.15%, 6/13, p=0.0005) re...

2011-01-01

253

Radioimmunoassay data processing, ch. 2

Purification of cyclotron-produced "2"0"3Pb for labeling Herceptin

The problems of radioimmunoassay data processing with a fairly big computer are investigated in detail. The logit transformation, introduced by Rodbard et al. in 1968, and the related mathematical operations are presented. From logit-ln plots the confidence limits for the calculation of the antigen concentration can be predicted as well as the minimum detectable dose (i.e. the sensitivity of the assay). The Scatchard plot is used to calculate the equilibrium constant of the antigen-antibody coupling reaction and the binding capacity. A 4-parameter logistic curve fitting method is developed and combined with a method to carry out a multi-component Scatchard analysis. Both a weighted and an unweighted linear regression procedure are evaluated for use in calculating the plasma renin activity from the angiotensin I amounts determined by radioimmunoassay. Improvements for Rodbard's computer programmes are proposed.

1978-02-15

254

No detectable XMRV in subjects with chronic fatigue syndrome from Quebec

A simple and rapid procedure was developed for the purification of cyclotron-produced "2"0"3Pb via the "2"0"3Tl(d,2n) "2"0"3Pb reaction. A Pb(II) selective ion-exchange resin, with commercial name Pb Resin from Eichrom Technologies, Inc., was used to purify "2"0"3Pb from the cyclotron-irradiated Tl target with excellent recovery of the enriched Tl target material. The purified "2"0"3Pb was used to radiolabel the monoclonal antibody Herceptin. The in vitro and in vivo properties of the "2"0"3Pb radioimmunoconjugate were evaluated.

2005-04-01

255

British Library Electronic Table of Contents (United Kingdom)

We investigated the presence of XMRV in a cohort of Quebec patients with chronic fatigue syndrome (CFS). DNA was purified from activated peripheral blood mononuclear cells (PBMCs) and PCR was used to detect XMRV gag and env in 72 patients. Anti-XMRV antibodies were searched in sera of 62 patients by Western blot analysis. Attempts to detect XMRV antigens was made, using immunofluorescence with Gag anti-p30 antiserum on activated PBMC from 50 patients. Plasma viremia was measured by RT-PCR on 9 subjects. Finally, detection of infectious virus in 113 CFS subjects was made by co-culture of PHA+IL-2 activated PBMC with human LNCaP carcinoma cells, and by infecting the same susceptible cells with plasma, using a reverse transcriptase (RT) assay as a readout in both experiments. No detection of ...

2011-01-01

256

Mutation analysis of KRAS prior to targeted therapy in colorectal cancer: development and evaluation of quality by a European external quality assessment scheme

British Library Electronic Table of Contents (United Kingdom)

In Europe, the use of anti-EGFR monoclonal antibodies is restricted to Kirsten RAS (KRAS) wild-type colorectal tumors. Information on the KRAS status of the patients tumor is thus key for clinical practice; however, there is little guidance or definition on which KRAS mutations to assess and how to assess them. To ensure the consistency and the quality of KRAS test results in Europe, an interlaboratory control network needs to be set up. This pilot study aimed to identify the variables that need to be assessed in a quality control scheme and to provide a first assessment in a selected set of laboratories. Fourteen different tumor cases were circulated between 13 laboratories by a central laboratory acting as the referent for the mutation status determination. This study illustrated that of...

2011-01-01

257

Leishmania major ascorbate peroxidase overexpression protects cells against reactive oxygen species-mediated cardiolipin oxidation

British Library Electronic Table of Contents (United Kingdom)

Heme peroxidases are a class of multifunctional redox-active proteins found in all organisms. We recently cloned, expressed, and characterized an ascorbate peroxidase from Leishmania major (LmAPX) that was capable of detoxifying hydrogen peroxide. Localization studies using green fluorescent protein fusions revealed that LmAPX was localized within the mitochondria by its N-terminal signal sequence. Subcellular fractionation analysis of the cell homogenate by the Percoll density-gradient method and subsequent Western blot analysis with anti-LmAPX antibody further confirmed the mitochondrial localization of mature LmAPX. Submitochondrial fractionation analysis showed that the mature enzyme (?3.6?kDa shorter than the theoretical value of the whole gene) was present in the intermembrane space ...

2008-01-01

258

Involvement of Rh blood group polypeptides in the maintenance of aminophospholipid asymmetry

Insight into Thyroid-Stimulating Autoantibody Interaction with the Thyrotropin Receptor N-Terminus Based on Mutagenesis and Re-Evaluation of Ambiguity in This Region of the Receptor Crystal Structure

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, "1"2"5I-azido-PS, and with an inhibitor of PS transport, "1"2"5I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

259

British Library Electronic Table of Contents (United Kingdom)

Background: Thyroid-stimulating autoantibodies (TSAb) bind to the thyrotropin receptor (TSHR) extracellular domain, or ectodomain (ECD), comprising a leucine-rich repeat domain (LRD) linked by a hinge region to the transmembrane domain (TMD). The LRD (residues 22???260; signal peptide 1???21) contains two disulfide-bonded loops at its N-terminus. In the crystal structure of the isolated LRD complexed with human TSAb monoclonal antibody (mAb) M22, N-terminal disulfide loop 1 (residues 22???30) could not be determined because of crystal disorder. Nevertheless, present crystal structure data are interpreted to exclude a role for the LRD N-terminal disulfide loops in the TSAb epitope(s), contradicting prior functional evidence of a role for these loops in TSAb function. Materials and Methods: ...

2011-01-01

260

Immunofluorescence analysis of the internal brain anatomy of Nereis diversicolor (Polychaeta, Annelida)

British Library Electronic Table of Contents (United Kingdom)

Comparative analyses of neuroanatomical characters can make valuable contributions to the inference of phylogenetic relationships. Whereas investigations in this field are numerous for arthropods, in-depth studies on other protostomes are sparse. Here, we provide a survey of the internal neuroarchitecture of the brain of the aciculate ragworm Nereis diversicolor (Polychaeta, Annelida). Descriptions are based on confocal laser scanning microscope analyses of brain sections labeled with the nuclear marker DAPI and antibodies raised against FMRF-amide, serotonin, and histamine. Autofluorescence of the nervous tissue has been utilized to further elucidate the anatomical structures of the brain. The architecture of two major brain compartments, i.e., the paired mushroom bodies and the central o...

2008-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Immune responses induced by spirochetal outer membrane lipoproteins and glycolipids

British Library Electronic Table of Contents (United Kingdom)

The class of Spirochetes comprises a wide array of clinically important pathogens, including Treponema pallidum causing syphilis as well as Borrelia burgdorferi, the agent of Lyme disease (LD). Diseases caused by spirochetes are characterized by specific sequelae of host reactions, and also by characteristic antibody response patterns. Over the last decades, research on the interaction of spirochetes with the hosts immune system had a strong emphasis on outer membrane lipoproteins. In fact, these structures have been convincingly shown to activate immune cells via CD14 and Toll-like receptor (TLR)-2, and recent data also indicate an interaction with lipopolysaccharide (LPS)-binding protein (LBP). In particular, the interaction of B. burgdorferi with TLR-2 could not only be demonstrated in ...

2008-01-01

262

Homologous radioimmunoassay for human prolactin

Heparin binding sites on Ross River virus revealed by electron cryo-microscopy

Although thee are descriptions of a range of radioimmunoassays for human prolactin in various biological fluids, only one of these is an homologous assay using human prolactin as the reference standard and tracer as well and an anti-human prolactin antiserum (Sinha, Y.N., Selby, F.W.; Lewis, U.; and Vanderlaan, W.P., 1973, J. Clin. Endocr., Vol. 36, 509). A homologous radioimmunoassay using human putuitary prolactin has been developed. The separation method is based on the double antibody solid phase system. Cross reactivity with human growth hormone (GH), placental lactogen (HPL), the pituitary protein hormones and prolactins of various species were studied as were values found in normal subjects in basal conditions and after a TRH injection. (author).

263

Global suppression of mitogen-activated ovine peripheral blood mononuclear cells by surface protein activity from Mycoplasma ovipneumoniae

Cell surface glycosaminoglycans play important roles in cell adhesion and viral entry. Laboratory strains of two alphaviruses, Sindbis and Semliki Forest virus, have been shown to utilize heparan sulfate as an attachment receptor, whereas Ross River virus (RRV) does not significantly interact with it. However, a single amino acid substitution at residue 218 in the RRV E2 glycoprotein adapts the virus to heparan sulfate binding and expands the host range of the virus into chicken embryo fibroblasts. Structures of the RRV mutant, E2 N218R, and its complex with heparin were determined through the use of electron cryo-microscopy and image reconstruction methods. Heparin was found to bind at the distal end of the RRV spikes, in a region of the E2 glycoprotein that has been previously implicated in cell-receptor recognition and antibody binding.

2005-02-20

264

British Library Electronic Table of Contents (United Kingdom)

Mycoplasma ovipneumoniae is associated with chronic non-progressive pneumonia of sheep and goats. As with many other mycoplasmas involved in animal diseases, protective immune responses have not been achieved with vaccines, even though antibody responses can be obtained. This study focuses on characterizing the interaction of M. ovipneumoniae with ovine PBMC using carboxy-fluorescein-succinimidyl-ester (CFSE) loading and flow cytometry to measure lymphoid cell division. M. ovipneumoniae induced a strong in vitro polyclonal suppression of CD4^+, CD8^+, and B blood lymphocyte subsets. The suppressive activity could be destroyed by heating to 60^oC, and partially impaired by formalin and binary ethyleneimine treatment that abolished its viability. The activity resided on the surface-exposed m...

2010-01-01

265

Establishment of functional primary cultures of heart cells from the clam Ruditapes decussatus

British Library Electronic Table of Contents (United Kingdom)

Heart cells from the clam Ruditapes decussatus were routinely cultured with a high level of reproducibility in sea water based medium. Three cell types attached to the plastic after 2?days and could be maintained in vitro for at least 1?month: epithelial-like cells, round cells and fibroblastic cells. Fibroblastic cells were identified as functional cardiomyocytes due to their spontaneous beating, their ultrastructural characteristics and their reactivity with antibodies against sarcomeric ?-actinin, sarcomeric tropomyosin, myosin and troponin T-C. Patch clamp measurements allowed the identification of ionic currents characteristic of cardiomyocytes: a delayed potassium current (I K?slow) strongly suppressed (95%) by tetraethylammonium (1?mM), a fast inactivating potassium current (I K?fas...

2011-01-01

266

Cytoplasmic p63 immunohistochemistry is a useful marker for muscle differentiation: an immunohistochemical and immunoelectron microscopic study

British Library Electronic Table of Contents (United Kingdom)

TP63, a member of the TP53 gene family, is a nuclear marker of myoepithelial cells. Antibody against p63 is frequently used to aid in the diagnosis of prostate carcinoma, as well as in the identification of myoepithelial cells in other tissues including the breast. p63 is also a marker for squamous cell carcinoma. Recently, it was found that all p53 family members are involved in regulating the process of muscle differentiation through the retinoblastoma (RB) protein. Ablation of these p53 family functions blocks the differentiation program and promotes malignant transformation by enabling cooperating oncogenes to transform myoblasts. We therefore studied p63 expression in a number of neoplasms with myogenic differentiation. Immunohistochemical staining for p63 was performed on paraffin se...

2011-01-01

267

Comparative biochemistry of betaine biosynthesis and accumulation in diverse dicot families

Biomagnetic separation of Salmonella Typhimurium with high affine and specific ligand peptides isolated by phage display technique

Salt stress elicits betaine accumulation to high levels in species from several diverse dicot families (Chenopodiaceae, Amaranthaceae, Convolvulaceae, Solanaceae, and Asteraceae). FAM-MS studies with deuterated precursors showed that species from all these families synthesize betaine from choline. Enzyme assays and immunotitration data showed that all accumulating species contained betaine aldehyde dehydrogenase (BADH) enzyme activity recognized by antibodies raised against purified BADH isolated from Spinacia oleracea. Immunoblotting indicated that the BADH monomer was in all cases of Mr {approx} 63,000. The similarity of BADH in the different species is consistent with a single evolutionary origin for the betaine pathway. This was supported by the presence in immunoblots of a cross-reacting band at Mr {approx} 63,000 in Magnolia x Soulangiana, a primitive angiosperm.

1989-04-01

268

{omega} meson production at the Time-Of-Flight spectrometer

Analyses of food-borne pathogens are of great importance in order to minimize the health risk for customers. Thus, very sensitive and rapid detection methods are required. Current conventional culture techniques are very time consuming. Modern immunoassays and biochemical analysis also require pre-enrichment steps resulting in a turnaround time of at least 24 h. Biomagnetic separation (BMS) is a promising more rapid method. In this study we describe the isolation of high affine and specific peptides from a phage-peptide library, which combined with BMS allows the detection of Salmonella spp. with a similar sensitivity as that of immunomagnetic separation using antibodies.

2007-04-15

269

X-ray production yield in standardized thick target PIXE

No abstract prepared.

2002-03-01

270

Utilizing Gorlovsk Basin anthracite in metallurgical production

No abstract prepared.

2003-07-01

271

British Library Electronic Table of Contents (United Kingdom)

The composition and physicochemical properties of Gorlovsk Basin anthracite are studied, with a view to expanding its metallurgical use. In industrial tests, anthracite is employed in smelting hot metal in cupola furnaces, in the production of carbonized products and briquets, in ferroalloy production, in sintering iron ore, and in batch for foundry coke.

2008-01-01

272

The PISA experiment-spallation products identified by Bragg curve spectroscopy

Synthetic and bioengineered products in nuclear medicine and drug delivery

No abstract prepared.

2004-07-01

273

Studies on the Processing of Seasoned Product Containing Egg Yolk.

No abstract prepared.

1999-07-01

274

Production of sup 203 Pb by proton irradiation of Bi

Full Text Available

1988-03-01

275

Production of reducing oligosaccharides from galactan biopolymers

Short note.

1991-01-01

276

Mass production of refractory oxide crystals: cubic zirconia

No abstract prepared.

2006-07-01

277

Ethanol Production from Olive Cake Biomass Substrate

Short communication

1993-03-01

278

Detection of a Quorum-Sensing Inhibitor from the Natural Products

Full Text Available

2009-02-01

279

Close-to-threshold {phi} production at COSY-11

Full Text Available

2008-02-01

280

Bioplastic production from agro-industrial waste.

No abstract prepared.

2002-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

281

An Aplication Effect of UV-Absorbent on The Indigo Dyed Products

PMID:21769853

2011-09-01

282

48 CFR 31.205-25 - Manufacturing and production engineering costs.

Full Text Available

1999-08-01

283

Quick separation of fission product molybdenum and gamma-rays of Mo-102

...false Manufacturing and production engineering costs. 31.205-25 Section...205-25 Manufacturing and production engineering costs. (a) The costs of manufacturing and production engineering effort as described in (1)...

2010-10-01

284

Sunscreen

Medline Plus

... Animal & Veterinary Cosmetics Radiation-Emitting Products Tobacco Products Drugs Home > Drugs > Resources for You > Information for Consumers (Drugs) Section ...

285

Olympic National Forest -Tentatively Suitable Land for Timber Production

... electrophoresis fission products gamma radiation gamma spectra half-life

286

... displays land designated as Tentatively Suitable Land For Timber Production for the Forest Plan....

287

...Surface Aerators Products and Equipment Find and compare a variety of surface aerators products and equipment on the world's largest environmental industry portal. ...

288

...Extraction Arms Products and Equipment Find and compare a variety of extraction arms products and equipment on the world's largest environmental industry portal. ...

289

Medical Isotope Production in the Fast Flux Test Facility and the High Flux Isotope Reactor and Isotope Production Software Users Guides

... Tunnel Composting Products and Equipment Find and compare a variety of tunnel composting products and equipment on the world's largest environmental industry portal. ...

290

JPRS Report. Soviet Union: World Economy & International ...

No abstract prepared.

2000-08-23

291

Integrating phytotechnologies with energy crop production for biofuels

... in the processes of production and, partic- ularly, product distribution and also by the increase in directive and disciplinary methods of intervention ...

1989-05-13

292

Large-scale production of biofuels through biomass (co)-gasification and Fischer Tropsch synthesis

Integrating phytotechnologies with energy crop production for biofuels, bioenergy, and bioproducts. In: Sixth International Phytotechnologies Conference ; ...

293

Extracranial-intracranial bypass surgery utilizing homologous arterial grafts irradiated with high voltage cathode rays. Experimental study and clinical application

In this work the production of sustainable syngas has been investigated for use in a co-production plant for electricity and Fischer Tropsch diesel at the Willem-Alexander Centrale in Buggenum, the Netherlands, and has also been compared to the co-production of SNG. From the analysis for this specific situation it is determined that the co-production of Fischer Tropsch liquids is economically more beneficial than co-production of SNG.

2002-07-01

294

Extracranial-intracranial bypass surgery utilizing homologous arterial grafts irradiated with high voltage cathode rays

Homologous and heterologous arterial segments were implanted in Fisher rats subcutaneously for the purpose of examining the antibody titer of the recipients' serum after implantation by means of the immune-adherence hemagglutination method. The antibody titer after implantation both of homologous and heterologous grafts decreased to 1/8 by 2.0 million (M) rads irradiation of high voltage cathode rays. The results suggested that high voltage cathode ray irradiation was not enough for heterologous graft to suppress its tissue reaction. Homografts taken from dogs 3 or 6 hours after sacrifice were irradiated with 2.0 M rads and transplanted in canine carotid artery using the technic of end-to-end anastomosis. Angiograms 6 months after operation revealed excellent patency rate in all the grafts of 28 dogs. furthermore, findings of the grafts from 1 week to 5 years after operation on scanning and transmission electron microscopies were ...

1982-06-01

295

Extracellular Protein Disulfide Isomerase Regulates Feedback Activation of Platelet Thrombin Generation Via Modulation of Coagulation Factor Binding.

Homologous and heterologous arterial segments were implanted in Fisher rats subcutaneously for the purpose of examining the antibody titer of the recipients' serum after implantation by means of the immune-adherence hemagglutination method. The antibody titer after implantation both of homologous and heterologous grafts decreased to 1/8 by 2.0 million (M) rads irradiation of high voltage cathode rays. The results suggested that high voltage cathode ray irradiation was not enough for heterologous graft to suppress its tissue reaction. Homografts taken from dogs 3 or 6 hours after sacrifice were irradiated with 2.0 M rads and transplanted in canine carotid artery using the technic of end-to-end anastomosis. Angiograms 6 months after operation revealed excellent patency rate in all the grafts of 28 dogs. furthermore, findings of the grafts from 1 week to 5 years after operation on scanning and transmission electron microscopies were evaluated. ...

1982-01-01

296

Comparative removal of pyrimidine dimers from human epidermal keratinocytes in vivo and in vitro

Background: Protein disulfide isomerase (PDI) controls platelet integrin function, tissue-factor (TF) activation, and concentrates at fibrin and thrombus formation sites of vascular injury. Objective: We investigated involvement of surface thiol isomerases and especially PDI, in thrombin-mediated thrombin amplification on human platelets. Methods/Results: Using a new developed thrombin-dependent platelet thrombin generation assay we observed that the feedback activation of thrombin generation on the platelet surface does not depend on TF, as anti-TF antibodies inhibiting TF-induced thrombin formation in platelet-depleted plasma had no effect compared to vehicle-treated controls. Feedback activation of thrombin generation in the presence of platelets was significantly diminished by membrane impermeant thiol blockers or by the thiol isomerase-inhibitors bacitracin and anti-PDI antibody RL90, respectively. Platelet thrombin formation depends on ...

2011-09-19

297

Comparative removal of pyrimidine dimers from human epidermal keratinocytes in vivo and in vitro

We have compared the kinetics for repair of UV-induced cyclobutane pyrimidine dimers in the DNA of keratinocytes in human skin and in cell culture. A small area of the buttocks of volunteers was exposed to UVB-irradiation and biopsies were taken at various time intervals. Epidermal keratinocytes in culture from the same subjects were exposed to UVC with doses chosen to elicit comparable yields of dimers in cellular DNA. The initial density of pyrimidine dimers and the kinetics of their removal were assessed utilizing the dimer-specific T4 endonuclease V and sedimentation of the unlabeled DNA through alkaline sucrose gradients. The position of DNA in the gradients was determined using a monoclonal antibody against random sequences of single-stranded DNA in a sensitive immunoassay. The initial dimer frequency was 3.9-6.7 per 10(8) daltons DNA. About 40% of the dimers were removed within 1 h, 70% in 6 h, and 90% in 24 h for both in vivo and in vitro samples. The early ...

1988-10-01

298

A single amino acid substitution (R441A) in the receptor-binding domain of SARS coronavirus spike protein disrupts the antigenic structure and binding activity

We have compared the kinetics for repair of UV-induced cyclobutane pyrimidine dimers in the DNA of keratinocytes in human skin and in cell culture. A small area of the buttocks of volunteers was exposed to UVB-irradiation and biopsies were taken at various time intervals. Epidermal keratinocytes in culture from the same subjects were exposed to UVC with doses chosen to elicit comparable yields of dimers in cellular DNA. The initial density of pyrimidine dimers and the kinetics of their removal were assessed utilizing the dimer-specific T4 endonuclease V and sedimentation of the unlabeled DNA through alkaline sucrose gradients. The position of DNA in the gradients was determined using a monoclonal antibody against random sequences of single-stranded DNA in a sensitive immunoassay. The initial dimer frequency was 3.9-6.7 per 10(8) daltons DNA. About 40% of the dimers were removed within 1 h, 70% in 6 h, and 90% in 24 h for both in vivo and in vitro samples. The early ...

1988-01-01

299

Fragranced consumer products: Chemicals emitted, ingredients unlisted

The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) has two major functions: interacting with the receptor to mediate virus entry and inducing protective immunity. Coincidently, the receptor-binding domain (RBD, residues 318-510) of SAR-CoV S protein is a major antigenic site to induce neutralizing antibodies. Here, we used RBD-Fc, a fusion protein containing the RBD and human IgG1 Fc, as a model in the studies and found that a single amino acid substitution in the RBD (R441A) could abolish the immunogenicity of RBD to induce neutralizing antibodies in immunized mice and rabbits. With a panel of anti-RBD mAbs as probes, we observed that R441A substitution was able to disrupt the majority of neutralizing epitopes in the RBD, suggesting that this residue is critical for the antigenic structure responsible for inducing protective immune responses. We also demonstrated that the RBD-Fc bearing R441A mutation could not ...

2006-05-26

300

British Library Electronic Table of Contents (United Kingdom)

Fragranced consumer products are pervasive in society. Relatively little is known about the composition of these products, due to lack of prior study, complexity of formulations, and limitations and protections on ingredient disclosure in the U.S. We investigated volatile organic compounds (VOCs) emitted from 25 common fragranced consumer products-laundry products, personal care products, cleaning supplies, and air fresheners-using headspace analysis with gas chromatography/mass spectrometry (GC/MS). Our analysis found 133 different VOCs emitted from the 25 products, with an average of 17 VOCs per product. Of these 133 VOCs, 24 are classified as toxic or hazardous under U.S. federal laws, and each product emitted at least one of these compounds. For ''green'' products, emissions of these c...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

On Finite Noncommutativity in Quantum Field Theory

Tissue culture process for the clonal production of loblolly pine plantlets

We consider various modifications of the Weyl-Moyal star-product, in order to obtain a finite range of nonlocality. The basic requirements are to preserve the commutation relations of the coordinates as well as the associativity of the new product. We show that a modification of the differential representation of the Weyl-Moyal star-product by an exponential function of derivatives will not lead to a finite range of nonlocality. We also modify the integral kernel of the star-product introducing a Gaussian damping, but find a nonassociative product which remains infinitely nonlocal. We are therefore led to propose that the Weyl-Moyal product should be modified by a cutoff like function, in order to remove the infinite nonlocality of the product. We provide such a product, but it appears that one has to abandon the ...

2010-01-01

302

Microsoft Word - FCRN intensive vs extensive livestock.doc

A tissue culture process, organogenesis, is described which involves the stepwise production of a shoot apex, which grows to have leaves and stem and finally roots, culminating in the production of a complete plant. The production and subsequent development of numerous adventitious shoots, preferably from adult tree tissues offers the greatest potential for commercial cloned plantlet production. The process describes these steps for Pinus taeda, the loblolly pine. (Refs. 25).

1981-02-01

303

LC-MS analysis in the e-beam and gamma radiolysis of metoprolol tartrate in aqueous solution: Structure elucidation and formation mechanism of radiolytic products

rapid rise in intensive production (particularly intensive pig and poultry production ) much of the expansion in land ...than extensively reared ruminant production, and intensive pig and poultry production is, by a long way, ...In short, intensive pig and poultry production can be seen, from this perspective, as the

304

British Library Electronic Table of Contents (United Kingdom)

E-beam and gamma products from the radiolysis of aqueous solutions of (+-)-metoprolol tartrate, saturated in nitrogen, are analyzed by HPLC with on-line mass and UV detectors. The structures of 10 radiolytic products common to e-beam and gamma irradiations are elucidated by comparing their fragmentation pattern to that of (+-)-metoprolol. Two of the radiolytic products are also metabolites. Different routes for the formation of the radiolytic products are proposed.

2006-01-01

305

Energy consumption scenarios for the fertilizer industry; Cenarios e consumo de energia na industria de fertilizantes

Regulatory compliance | Mott MacDonald

This paper shows the characteristics of the fertilizer production park of the State of Minas Gerais, Brazil, mainly in the aspects of energy consumption and energy sources used in the production process of the various kinds of fertilizers and by-products. A scenario including the fertilizer production evolution was made, relating this future production with the future energy consumption of this sector. 7 figs., 2 tabs.

1991-12-31

306

On the probability of flucton production in the 1p-shell nuclei

...broad range of experience in designing and building research and development laboratories for products such as vaccines, blood and sera, biological products, medical devices, solid dosage forms , highly potent products and cytotoxic material. We also have experience in a range of modular laboratories for TSE research, ...

307

Probabilities of flucton production have been calculated for the 1p-shell nuclei and its modification by the Yastrov method. The results obtained are in a qualitative agreement with the data resulting from the analysis of experiment in an assumption of a flucton mechanism of proton-nuclear inclusive reactions with the production of cumulative particles.

308

Metabolic Engineering of Lactobacillus plantarum for Production of l-Ribulose?

...In your area Get connected Farmers & growers news Training & events Poultry (laying) Poultry production can be a profitable enterprise for organic farmers and eggs are ... To find out more about organic poultry production download our free comprehensive introductory guide: Organic poultry production - an introductory guide [PDF, ...

309

Product identification in industrial batch fermentation using a variable forgetting factor

l-Ribulose is a rare and expensive sugar that can be used as a precursor for the production of other rare sugars of high market value such as l-ribose. In this work we describe a production...Full Text Available

2007-11-01

310

British Library Electronic Table of Contents (United Kingdom)

For reliable operation and the optimization of production, industrial fermentation processes require appropriate tools for monitoring the process in real time. This work presents the structure and operation of a soft sensor for the on-line monitoring of biomass and product concentration during salinomycin and bacitracin fermentation in an industrial, 80-m^3 batch reactor; moreover it provides a tool for evaluation of batch production verified in industrial application. The process estimation algorithm consists of decoupled growth and product models, which ensures an unbiased convergence of the estimator and the robustness of the model. The production of secondary metabolites is described with a non-structured model upgraded with a variable forgetting factor that demonstrated a successful e...

2011-01-01

311

EXPORTS AND PRODUCTIVITY: MOROCCAN MANUFACTURING 1985-1995

...Rolls Royce Production Plant, Goodwood Rolls Royce Production Plant, Goodwood Rolls Royce Production Plant, Goodwood Browser does not support script. Home ...Wilson Group plc »Projects »Buildings & Infrastructure »Industrial & Distribution »Rolls Royce Production Plant, Goodwood Rolls Royce Production Plant, Goodwood Project Introduction Scott Wilson was ...Robin Hood Airport Robin Hood Airport Doncaster Sheffield Rolls Royce Production Plant, Goodwood Rolls-Royce plc Rolls-Royce plc, New Manufacturing Facility Royal Arsenal Development ...

312

British Library Electronic Table of Contents (United Kingdom)

ABSTRACT The relation between exports and productivity is analysed for the case of Morocco using annual panel data for the years 1985-1995 covering six large urban areas and 18 manufacturing sectors. In the empirical analysis two main features are distinguished, i.e. productivity differentials and export externalities. The former is the most dominant one for Morocco, i.e. sectors with low labour productivity export most and within-sectors exporting firms are more productive than nonexporting firms. Regarding the latter, only weakly significant evidence is found of both sector wide or within sector productivity externalities as a result of exporting.

2006-01-01

313

Application of the exergy method to environmental impact estimation: The ammonium nitrate production as a case study

Using light to bioactivate surfaces: A new way of creating oriented, active immunobiosensors

The exergy method is used to compare different production processes and various methods for emission abatement with respect to their overall environmental impact. Some ammonium nitrate production processes are studied as examples, because the pollutants (ammonia and ammonium nitrate), emitted from these processes into the air and/or into the water, are really a feedstock and a product from the production process. Therefore, the essential result of the waste flows treatment is the recycling of the pollutants (ammonia and ammonium nitrate) back into the production process, decreasing simultaneously the exergy input and cumulative exergy consumption. (author)

2010-08-15

314

Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells

Ultraviolet light can be used to immobilize biomolecules onto thiol reactive surfaces in order to, e.g., make biosensors. The mechanism involves light-induced formation of free, reactive thiol groups in disulphide containing molecules. This technology allows for the creation of arrays of biomolecules with a high degree of reproducibility, circumventing the need for often expensive nano/micro-dispensing technologies. The ultimate size of the immobilized spots is defined by the focal area of the UV beam. Light-induced immobilization has the added benefit that the immobilized molecules will be spatially oriented and covalently bound to the surface. In this paper, we demonstrate the utility of a sensor array created with the new sensor technology when integrated into a microfluidic system. Protein arrays made using light-induced immobilization showed successful antigen/antibody binding in a flow cell allowing the visualisation of real time binding and enzyme activity. ...

2007-12-15

315

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation

The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10 nM of Cd for 24 h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10 nM. By two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The present study demonstrated the morphological effects as well as affected proteins in ...

2008-11-20

316

British Library Electronic Table of Contents (United Kingdom)

Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24?h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western bl...

2008-01-01

317

Positive IgG Western Blot for Borrelia burgdorferi in Colombia

Scientific Electronic Library Online (English)

Abstract in english In order to evaluate the presence of specific IgG antibodies to Borrelia burgdorferi in patients with clinical manifestations associated with Lyme borreliosis in Cali, Colombia, 20 serum samples from patients with dermatologic signs, one cerebrospinal fluid (CSF) sample from a patient with chronic neurologic and arthritic manifestations, and twelve serum samples from individuals without clinical signs associated with Lyme borreliosis were analyzed by IgG Western blot. The (more) results were interpreted following the recommendations of the Centers for Diseases Control and Prevention (CDC) for IgG Western blots. Four samples fulfilled the CDC criteria: two serum specimens from patients with morphea (localized scleroderma), the CSF from the patient with neurologic and arthritic manifestations, and one of the controls. Interpretation of positive serology for Lyme disease in non-endemic countries must be cautious. However these results suggest that ...

1999-07-01

318

Platelet thrombosis in cardiac-valve prostheses

Peru-15, an improved live attenuated oral vaccine candidate for Vibrio cholerae O1.

The contribution of platelets and clotting factors in thrombosis on cardiovascular prostheses had been quantified with several tracers. Thrombus formation in vivo could be measured semiquantitatively in animal models and patients with indium-111, Technetium-99m labeled platelets, iodine-123, iodine-131 labeled fibrinogen, and In-111 and Tc-99m labeled antibody to the fibrinogen-receptor on the platelet- membrane, or fibrin. The early studies demonstrated that certain platelet-inhibitors, e.g. sulfinpyrazone, aspirin or aspirin- persantine increased platelet survival time with mechanical valves implanted in the baboon model and patients. Thrombus localization by imaging is possible for large thrombus on thrombogenic surface of prosthesis in the acute phase. The majority of thrombus was found in the sewing ring (Dacron) in the acute phase in both the mechanical and tissue valves. The amount of retained thrombus in both mechanical and tissue valves in our one-day ...

1989-06-08

319

Online immunoaffinity LC/MS/MS. A general method to increase sensitivity and specificity: How do you do it and what do you need?

Cholera vaccine candidate Peru-15 was derived from a Vibrio cholerae O1 El Tor Inaba strain by deleting the cholera toxin genetic element, introducing the gene encoding cholera toxin B subunit into recA, and screening for nonmotility. In a controlled study, Peru-15 (2 x 10(8) cfu) was administered to 11 volunteers. No vaccinee developed diarrhea, and 10 of 11 had > 4-fold rises in vibriocidal antibody titers. One month later, 5 vaccinees and 5 control volunteers were challenged with wild type V. cholerae O1. Four of 5 controls developed diarrhea (mean, 1.9 L). Two Peru-15 vaccinees developed diarrhea, 1 with < 0.3 L and 1 with approximately 1.0 L; this latter volunteer had not developed a significant vibriocidal immune response to vaccination. Peru-15 shows promise as a single-dose, oral cholera vaccine that is safe, immunogenic, and protective. PMID:7561195

1995-10-01

320

Ixodes ricinus: vector of a hitherto undescribed spotted fever group agent in Switzerland.

There is an increased emphasis on hyphenated techniques such as immunoaffinity LC/MS/MS (IA-LC/MS/MS) or IA-LC/MRM. These techniques offer competitive advantages with respect to sensitivity and selectivity over traditional LC/MS and are complementary to ligand binding assays (LBA) or ELISA's. However, these techniques are not entirely straightforward and there are several tips and tricks to routine sample analysis. We describe here our methods and procedures for how to perform online IA-LC/MS/MS including a detailed protocol for the preparation of antibody (Ab) enrichment columns. We have included sample trapping and Ab methods. Furthermore, we highlight tips, tricks, minimal and optimal approaches. This technology has been shown to be viable for several applications, species and fluids from small molecules to proteins and biomarkers to PK assays. PMID:21872661

2011-08-22

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Isolation of fetal DNA from nucleated erythrocytes in maternal blood

A tick/rickettsial survey in various parts of Switzerland revealed the presence of a new, hitherto undescribed spotted fever group rickettsia ("Swiss agent") in up to 11.7% of I. ricinus collected off vegetation. Infection in ticks was found to be generalized with rickettsiae developing intracellularly and occasionally also intranuclearly. As a result of massive growth in ovarial tissues, including the germinative cells, the rate of transovarial and filial infection was 100%. The "Swiss agent" appears to be nonpathogenic for guinea pigs, domestic rabbits, and Swiss mice, but in male meadow voles (Microtus pennsylvanicus) it produces a microscopically detectable infection in the tunica vaginalis. The rickettsia grows well in tissue culture systems including chick embryo fibroblast, Vero, and vole tissue cells, when inoculated via yolk sac into 5-day-old hens' eggs, it kills 100% of the embryos after 5 to 7 days. Antigenic relatedness of the "Swiss agent" to rickettsiae of the spotted ...

1979-12-01

322

Intracellular monitoring of superoxide dismutase expression in an Escherichia coli fed-batch cultivation using on-line disruption with at-line surface plasmon resonance detection.

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. The authors used monoclonal antibody against the transferrin receptor (TIR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, they were successful in detecting the Y chromosomal sequence in 75% of ...

1990-05-01

323

In-situ synthesis of poly(dimethylsiloxane)-gold nanoparticles composite films and its application in microfluidic systems.

An on-line cell disruption system for at-line monitoring of the intracellular concentration of recombinant human superoxide dismutase (rhSOD) in a genetically modified Escherichia coli strain, HMS174(DE3) (pET11a/rhSOD), in bioreactor cultivations is described. The sampled bacteria were disrupted on-line by rapid mixing with a nonionic detergent. The recombinant protein content of the lysed bacterial sample was quantitated by a subsequent surface plasmon resonance biosensor with a specific monoclonal antibody. Extraction efficiency of the monitoring system was optimized with respect to the flow rate ratio of the cell suspension and the detergent at relevant cell densities with the aim to attain rapid monitoring. Monitoring was demonstrated for a shake flask culture and a glucose-limited fed-batch cultivation. The results are compared with a traditional enzyme-linked immunosorbent assay method showing a correlation coefficient of R2 = 0.97. Extraction efficiency of ...

2005-07-01

324

Immunolocalization of endothelin-B receptor in mouse intestinal tract.

We presented a simple approach for in-situ synthesis of poly(dimethylsiloxane) (PDMS)-gold nanoparticles composite film based on the special characteristics of PDMS itself. It is an environmentally safe synthesis method without the requirement of additional reducing/stabilizing agents. The region where the resulting gold nanoparticles distribute (in the matrix or on the surface of the polymer) and the size of the nanoparticles, as well as the colour of the free-standing films, can be simply controlled by adjusting the ratio of curing agent and the PDMS monomer. The chemical and optical properties of these composite films were studied. Using such a method, gold nanoparticle micropatterns on PDMS surfaces can be performed. And based on the gold nanoparticles micropattern, further modification with antibodies, antigens, enzymes and other biomolecules can be achieved. To verify this ability, an immobilized glucose oxidase (GOx) reactor in microchannels was built and ...

2007-11-14

325

Hepatic aflatoxin B1-DNA adducts and TP53 mutations in patients with hepatocellular carcinoma despite low exposure to aflatoxin B1 in southern Japan

The endothelin-B (ETB) receptor is a G-protein-coupled receptor that binds endothelin ligands and is essential for the development of epidermal melanocytes and enteric neurons. Recent reports indicate that ETB is localized to nuclei in cardiac ventricular myocytes, although it has been thought that ETB is localized mainly on the plasma membrane. It remains unknown, however, whether this unique distribution of ETB occurs in other tissues. To elucidate the subcellular distribution of ETB in the intestine, we performed immunofluorescence of ETB in mouse intestine using a specific antibody. ETB-like immunoreactivity was detected in both the mucosal and muscle layers. In the mucosal layer, villous epithelial cells, stromal cells of the lamina propria, and cryptic cells were immunostained. Subcellularly, ETB is localized mainly to the nuclei of villous epithelial cells. In the muscle layer, immunoreactivity of ETB was localized to the myenteric plexus. These findings ...

2004-11-01

326

British Library Electronic Table of Contents (United Kingdom)

Abstract Background & aims: Hepatitis B or C virus infection is considered to be the main cause of hepatocellular carcinoma (HCC) in Japan. Aflatoxin B1 (AFB1) is a carcinogen associated with HCC in regions with high exposure. Mutations in codon 249, exon 7 are a hallmark of AFB1 exposure. Therefore, to clarify the role of AFB1 in hepatocarcinogenesis, we examined AFB1-DNA in liver tissue and sequenced TP53 in Japanese patients with HCC. Methods: Hepatocyte AFB1-DNA adducts were determined immunohistochemically and direct sequencing of TP53 was done to determine mutations in 188 of 279 patients who underwent hepatic resection for HCC. We assessed hepatitis C virus antibodies (HCV Ab) and HBSAg expression; patients without either were defined as having non-B non-C hepatocellular carcinoma (...

2011-01-01

327

Gd-25 DTPA-MAb, a potential NMR contrast agent for MRI in the xenografted nude mouse: preliminary studies

FMRFamide-like immunoreactivity in rat brain: development of a radioimmunoassay and its application in studies of distribution and chromatographic properties

Monoclonal antibodies (MAbs) 19-9 and 73-3 specific for human colon adenocarcinoma were labelled with a high number of gadolinium atoms. Twenty five DTPA were chelated per MAb, with only slight loss of immunoreactivity. The NMR contrast agent Gd-25 DTPA-MAb 19-9 or 73-3 ((Gd) 17 mumole/kg, (MAb) 60 microM) was injected into nude mice bearing human colon adenocarcinoma (SW948). Tumors were removed 24 hr after injection and T1 was measured in vitro. T1 relaxation time varied according to MAb specificity against tumour targets; T1 decreased 20% for MAb 19-9 and MAb 73-3 with SW948 tumor. Imaging was performed with this model. Very good contrast was obtained 24 hr after Gd-25 DTPA-MAb injection.

1988-01-01

328

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

A radioimmunoassay is described for the molluscan neuropeptide, Phe-Met-Arg-Phe-NH"2(FMRFamide). The antibody used is C-terminal-specific and shows slight but significant (1-2%) cross-reactivity with chicken pancreatic polypeptide (APP). The assay has been used to identify in rat brain extracts a pair of molecules that may represent mammalian counterparts of FMRFamide. Their concentrations were highest in spinal cord and hypothalamus (>10 pmol.g"-_1) and lowest in cerebellum and striatum (<3.5 pmol.g"-_1). The two immunoreactive peptides were separated on CM ion-exchange chromatography where they appeared to be less basic than FMRFamide. On Sephadex G50 gel filtration one eluted in a similar position to FMRFamide and the other slightly earlier suggesting it may be of higher molecular weight. The rat immunoreactive components do not correspond to previously described neuropeptides or hormones, and may be members of a new group of mammalian neuropeptides with ...

329

Bevacizumab as a treatment option for radiation-induced cerebral necrosis

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in situ hybridization of a cloned fragment to ...

1988-10-01

330

A versatile and potentially general approach to the targeting of specific cell types by retroviruses: Application to the infection of human cells by means of major histocompatibility complex class I and class II antigens by mouse ecotropic murine leukemia virus-derived viruses

Radiation necrosis of normal CNS tissue represents one of the main risk factors of brain irradiation, occurring more frequently and earlier at higher total doses and higher doses per fraction. At present, it is believed that the necrosis results due to increasing capillary permeability caused by cytokine release leading to extracellular edema. This process is sustained by endothelial dysfunction, tissue hypoxia, and subsequent necrosis. Consequently, blocking the vascular endothelial growth factor (VEGF) at an early stage could be an option to reduce the development of radiation necrosis by decreasing the vascular permeability. This might help to reverse the pathological mechanisms, improve the symptoms and prevent further progression. A patient with radiation-induced necrosis was treated with an anti-VEGF antibody (bevacizumab), in whom neurologic signs and symptoms improved in accordance with a decrease in T1-weighted fluid-attenuated inversion recovery signals. ...

2011-02-01

331

The sites and topology of mitochondrial superoxide production

A technique for delivering genes carried by recombinant retroviruses into specific cell types could have numerous applications in oncology, developmental biology, and gene therapy. As a first step toward this remote goal the authors designed a procedure allowing in vitro cell targeting by retroviruses. Biotinylated antibodies against the viral envelope protein on one side, and against specific cell membrane markers on the other side, were bridged by streptavidin and used to link the virus to the host. The method was successfully used to infect human cells with ecotropic murine retroviruses by means of major histocompatibility complex class I and class II antigens and appears easily adaptable to other cell, membrane markers. Moreover, the sequential protocol they design, although allowing infection of human cells, requires less stringent safety constraints than would handling of amphotropic virus stocks.

1989-12-01

332

The Product Operator Formalism: A Physical and Graphical Interpretation

Mitochondrial superoxide production is an important source of reactive oxygen species in cells, and may cause or contribute to ageing and the diseases of ageing. Seven major sites of superoxide...Full Text Available

2010-08-01

333

Single leptoquark production at [ital e][sup +][ital e[minus

The product-operator formalism is the most commonly used tool for describing and designing multidimensional NMR experiments. In spite of its relative simplicity and sound theoretical underpinnings,...Full Text Available

2010-03-01

334

Preannouncing pioneering versus follower products: what should the message be?

We consider single production of leptoquarks (LQ's) at [ital e][sup +][ital e[minus

1994-04-01

335

British Library Electronic Table of Contents (United Kingdom)

Despite the high practical relevance, prior research does not provide a clear picture whether the effectiveness of new product preannouncements is contingent upon order of entry and whether the message content of preannouncements for pioneering products should be different from those for followers. Drawing on diffusion research, the authors examine how preannouncements that focus on risk reduction and the product?s relative advantage influence the relationship between preannouncement intensity and new product success, taking into account order of entry. A cross-industry study investigating 151 new product launches shows that for pioneers, a message focus aimed at reducing perceived product risk positively influences preannouncement effectiveness. Furthermore, a relative advantage focus neg...

2009-01-01

336

Olympic National Forest -Suitable Land for Timber Production

Oceanographic Processes and Marine Productivity in Waters Offshore ...

Map layer displays land designated as Suitable Land For Timber Production for the Forest Plan. It consists of all National Forest Lands less Non-forested ... ...

337

New equipment recharges Oldham batteries

Jun 16, 2011 ... Title: Chapter 21: Oceanographic Processes and Marine Productivity in Waters Offshore of Marbled Murrelet Breeding Habitat ...

338

The battery manufacturing industry has not been immune to the impact of new technologies. How one company is adapting production techniques to meet the demand for better products is discussed.

1982-09-24

339

Introducing N-glycans into natural products through a chemoenzymatic approach**

...Air Quality Monitoring Networks Products and Equipment Find and compare a variety of air quality monitoring networks products and equipment on the world's largest environmental industry portal. ...

340

If I Had - Excessive Skin Aging

The present study describes an efficient chemoenzymatic method for introducing a core N-glycan of glycoprotein origin into various lipophilic natural products. It was found...Full Text Available

2008-11-24

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341

Medline Plus

... some home products, like vitamin A, glycolic acid, antioxidants, vitamin C, etc. We can use these products ...

342

Health and safety risks in production agriculture.

Hadron Calorimeter Endcap

Production agriculture is associated with a variety of occupational illnesses and injuries. Agricultural workers are at higher risk of death or disabling injury than most other workers. Traumatic injury...Full Text Available

1998-10-01

343

Factoring Potential Accelerator Transmutation of Waste Demonstrations into Accelerator Production of Tritium Design Planning

Development of the Technology and Control of Electron-Beam Welding Procedure and Production of Full Scale Sector of Endcap CMS Hadron Calorimeter. Preparation for Mass Production.

344

Exposure data for personal care products: Hairspray, spray perfume, liquid foundation, shampoo, body wash, and solid antiperspirant

Factoring Potential Accelerator Transmutation of Waste Demonstrations into Accelerator Production of Tritium Design Planning

1998-01-01

345

British Library Electronic Table of Contents (United Kingdom)

Reliable exposure information for cosmetic and other personal care products and ingredients is needed in order to conduct safety assessments. Essential information includes both the amount of product applied, and the frequency of use. To obtain current data, studies to assess consumer use practices were undertaken. Six widely used personal care product types were included in the studies. Five of the products were cosmetics (spray perfume, hairspray, liquid foundation, shampoo, body wash) and one product was a cosmetic/over-the-counter drug product (solid antiperspirant). Three hundred and sixty women, ages 19-65 years, who regularly use the products of interest, were recruited at 10 different geographical locations within the US. The number of recruits was chosen to ensure a minimum of thr...

2006-01-01

346

Department of the

Corrosion behaviour of titanium alloys in work conditions of wet electric filters in sulfuric acid production

Waste Technology and Innovation Study Final Report ...Waste Technology and Innovation Study Final Report 21/18569/150554 ...Survey of waste and resource recovery related technology and innovation, including products and product uses

347

Closed Cycle Bleach Kraft Pulp Production; NICE3 Forest Products Project Fact Sheet

... filters molybdenum alloys production sulfuric acid alloys chemical reactions ...

1976-03-01

348

Age and the Thyrotropin Response to Hypothyroxinemia

Fact sheet written for the NICE3 Program on a new chlorine-free process for pulping and bleaching wastewater.

2000-10-30

349

Age Preservation of the Syntactic Processor in Production

Context: Some studies suggest altered pituitary functioning and TSH production with aging.Objective: Our objective was to test the hypothesis that less TSH production...Full Text Available

2010-08-01

350

Energy and cost analysis of organic fertilizer production in Nigeria

Two experiments are reported on the influence of cognitive aging on grammatical choice in language production. In both experiments, participants from two age-groups (young and old) produced...Full Text Available

2003-09-01

351

Volatilization of fluorides from solid uranium(IV)fluoride

Energy study was conducted in an organic fertilizer production plant in Nigeria, to determine the energy consumption patterns and the associated costs for the production of both powdered and pelletised fertilizer. Analysis was conducted for a daily production of 9000 kg of the finished products. Eight and nine defined unit operations were required for production of powder and pellets, respectively. The electrical and manual energy required for the production of powder were 94.5 and 5.6% of the total energy, respectively, with corresponding 93.9 and 5.1% for the production of pellets. The respective average energy intensities were estimated as 0.28 and 0.35 MJ/kg for powder and pellets. The most energy intensive operation was identified as the pulverizing unit with energy intensity of 0.09 MJ/kg, accounting for respective proportions of 33.4 ...

2010-01-15

352

The Factors for the Formation of Carcinogenic N-Nitrosamine from Dried Marine Food Products

... deposition evaporation fission products fluorides iodine 134 laboratory

1971-02-01

353

Study on Present Status of Poultry Farming and Improvement of Technical Management for Poultry Production

Full Text Available

1994-05-01

354

Studies on Oleoresin Product from Spices 3. Rapid Processing of Garlic Oleoresin

Full Text Available

1981-10-01

355

Studies on Oleoresin Product from Spices 2. Quality Stability of Red Pepper Oleoresin

Full Text Available

1993-02-01

356

Studies on Oleoresin Product from Spices 1. Extraction of Red Pepper Oleoresin

Full Text Available

1991-12-01

357

Studies of {eta} production in proton proton collisions with the time of flight spectrometer

Full Text Available

1991-12-01

358

Short-rotation poplar bark utilization for the production of light briquettes

No abstract prepared.

2002-03-01

359

Response Surface Analysis for the Production of an Enantioselective Lipase from Aspergillus niger by Solid-State Fermentation

Short communication. 4 refs.

1996-12-31

360

Full Text Available

2009-10-01

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361

Pure Scandium Production

Production and mechanical properties of some Fe-based metallic glass wires

Development of the Technology to Obtain Pure Metal Scandium by Thermal Decomposition of Scandium Iodide

362

Pretreatment of Waste Newspaper Using Ethylene Glycol for Bioethanol Production

No abstract prepared.

2002-07-01

363

Potential Detection of Irradiated Dried Agricultural Products by Viscosity Measurement

Full Text Available

2010-12-01

364

Possibility of production of new superheavy nuclei in complete fusion reactions

Full Text Available

1999-10-01

365

Physicochemical Characteristics of Waste Catalyst and Their In-Process Products from Recycling

... of Sciences (Poland) [5.48 Megabytes] NUCLEAR PHYSICS AND RADIATION

2008-09-01

366

Phenomenological analysis of K{sup +}-meson production in proton-nucleus collisions

Full Text Available

2011-04-01

367

Optimization of Enzymatic Pretreatment for the Production of Fermented Ginseng using Leaves, Stems and Roots of Ginseng

No abstract prepared.

2002-03-01

368

Optimization of Culture Condition for the Hydrocinnamic Acid Production from Bacillus subtilis IJ-31

Full Text Available

2010-03-01

369

Oospore Production in Broth Media and Oospore Germination of Phytophthora capsici

Full Text Available

2005-09-01

370

Monitoring Coastal Marshes for Persistent Saltwater Intrusion

Full Text Available

2009-06-01

371

Microstructure Change of Large Cast-forged Product by Forging and Heat Treatment Conditions

Primary goal: Provide resource managers with remote sensing products that support ecosystem

2009-01-01

372

Meat-Fat Tail Breed of Sheep

Full Text Available

2009-10-01

373

Manufacturing Characteristics of Environmental-friendly Waste Ash Brick with Industrial By-Products

Creation of Meat and Fat Tail Breed Sheep and Production of Ecologically Pure Mutton and Lamb

374

Manufacture of Spent Layer Chicken Meat Products by Natural Freeze-Drying during Winter

Full Text Available

2009-06-01

375

MICROSEISMICS AND GEOTECHNICAL APPLICATIONS

Full Text Available

2010-04-01

376

... PRODUCTION OF TIGHT OIL-GAS BEARING ROCKS, AND IS ALSO EMPLOYED FOR THE DEVELOPMENT OF GEOTHERMAL RESERVOIRS. ...

1989-06-01

377

Inside - NASA

Influence of production technology and irradiation conditions on radiation hardening of reactor vessel steels

developed for our government and industrial robots were used by .... UUV capabilities, into a product. The licensing agreement and ...

378

Improving the Quality of Life

Short communication.

1992-10-27

379

...and supply ensuring better food quality for the consumer developing safer and more flexible food production processes and technologies raw materials for food production; production and processing systems; by-products and wastes from fisheries and agro-industry; new food sources; packaging systems; quality monitoring; traceability Food contamination detecting and eliminating infectious and toxic agents throughout the food chain establishing the hazards and origins of food contaminants producing food more safely rapid ...

380

I. Introduction

Hydrogen production for better nuclear utilization

as a by-product during the purification of molten magnesium chloride prior to its electrolysis and during the

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Human Issues in Manufacturing Technology

... no. 2) p. 27-28. economics hydrogen power reactors nonmetals (ELEMENTS

1972-08-22

382

HIGH DUCTILE NANOPARTICLE DISPERSION METALLIC GLASS AND ITS PRODUCTION

... qualified manufacturing employees. David Lichtinger, plant manager for Lord Corporation's aerospace products plant in ...

1992-09-01

383

J-STORE (Japan)

Full Text Available

2003-01-17

384

Glossitis of military workingn> dogs in Vietnam: experimental production of tongue lesions.

PMID:4214371

1974-10-01

385

Fuel wood yarder

Entomopathogenic Nematodes in Biological Control

A small cable yarding system for accumulations of slash on forested land and overstocked non-productive stands is described.

1984-01-01

386

Effects of Coulomb and kaon nuclear potentials on soft K{sup +} production from nuclei

Commercial Production and Development, in Entomopathogenic Nematodes in Biological Control

387

Effect of Carbon Source Consumption Rate on Lincomycin Production from Streptomyces lincolnensis

No abstract prepared.

2002-03-01

388

ESR spectroscopy of intermediate products of water radiolysis; Spektroskopia EPR produktow posrednich radiolizy wody

Full Text Available

2004-06-01

389

Diquarks in gamma-p Collisions at LHeC

No abstract prepared

1998-12-31

390

Development of Regional Noodles Using Agricultural and Fishery Products of Cheju Island

This note studies the single production of scalar or vector diquarks at the LHeC collider.

2011-01-01

391

DEVELOPMENT OF CONTINUOUS SOLVENT EXTRACTION PROCESSES FOR COAL DERIVED CARBON PRODUCTS

Full Text Available

1996-08-01

392

Culture Conditions of Geobacillus kaustophilus DSM 7263 for Production of Thermophilic Extracellular Lipase

The purpose of this DOE-funded effort is to develop continuous processes for solvent extraction of coal for the production of carbon products. These carbon products include materials used in metals smelting, especially in the aluminum and steel industries, as well as porous carbon structural material referred to as ''carbon foam'' and carbon fibers. During this reporting period, efforts have focused on the facility modifications for continuous hydrotreating, as well as developing improved protocols for producing synthetic pitches.

2005-08-11

393

Comparative study of PTFE-g-DMAA copolymer obtained from PTFE production in IPEN and commercial PTFE

Full Text Available

2010-06-01

394

Community Structure, Productivity, and Nutrient Uptake of the Vascular Plants in the Wetlands of the Asan-Lake

Short communication.

1992-07-01

395

Biocontrol Agents of Crops Diseases

Full Text Available

2000-06-01

396

Scale up of Technology for Production and Application of Rhizobacteria-Based Preparations as Biocontrol Agents of Crop Diseases

397

BC

Antimutagenic effects of browning products reacted with polyphenol oxidase extracted from peach

as a by-product during the purification of molten magnesium chloride prior to its electrolysis and during the

398

About contaminations at the mass separator for fission products LOHENGRIN

Full Text Available

1992-04-01

399

Usage pattern of personal care products in California households

Published in summary form only.

1992-01-01

400

British Library Electronic Table of Contents (United Kingdom)

Given the concern over the potential for health risks associated with certain ingredients (e.g., phthalates) in personal care products, usage patterns of 30 types of personal care products (e.g., shampoo, sunscreen, fragrance, etc.) were collected in 604 California households through a telephone interview. Preferences in selecting products, e.g., scented or unscented, aerosol, and brand loyalty, were also investigated. Participants were recruited in three age groups, children (mostly preschoolers), their parents, and adults age 55 or older. Use frequencies of various product types varied by sex, age group, race, education, and climatic region. Product use by parent and child from the same household were correlated. Use frequencies of products in the same class (e.g., skincare) were moderat...

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Integrated coke, asphalt and jet fuel production process and apparatus

Product yield and hydrogen consumption selectivity tests for coal-liquefaction-catalyst development

A process and apparatus for the production of coke, asphalt and jet fuel m a feed of fossil fuels containing volatile carbon compounds therein is disclosed. The process includes the steps of pyrolyzing the feed in an entrained bed pyrolyzing means, separating the volatile pyrolysis products from the solid pyrolysis products removing at least one coke from the solid pyrolysis products, fractionating the volatile pyrolysis products to produce an overhead stream and a bottom stream which is useful as asphalt for road pavement, condensing the overhead stream to produce a condensed liquid fraction and a noncondensable, gaseous fraction, and removing water from the condensed liquid fraction to produce a jet fuel-containing product. The disclosed apparatus is useful for practicing the foregoing process. the process provides a useful method of mass producing and jet ...

1991-01-01

402

The Use of Virtual Reality Techniques during the Design Process: from the Functional Definition of the Product to the Design of its Structure

Because hydrogenation of coal to liquid products (oils) is accompanied by distributions of complex by-product mixtures (IOM, preasphaltenes, asphaltenes and gases) which change as a function of reaction variables (time, temperature and pressure) and reactor configuration, the determination of selectivity relationships for coal liquefaction catalysts has been a difficult and time-consuming task involving numerous experiments to adequately describe catalyst performance over a range of conditions. This paper describes a method for analyzing the experimental results of coal liquefaction reactions which may be applied to a number of aspects of coal liquefaction research and process control, including: rapid selectivity and performance screening for catalysts; correlation of laboratory results with process parameters; and optimization of product yield for plant process conditions. Catalyst selectivity and performance screening ...

1981-01-01

403

British Library Electronic Table of Contents (United Kingdom)

During the design process, designers have to define the structure of their product while considering its functional definition. This design phase remains little assisted for designers. In addition, as numerous options can be considered for the end of life of a product (reuse, remanufacturing, recycling,) it becomes more difficult to obtain a compromise concerning the final structure of the product. In this paper, we will show how the use of virtual reality helps designers to transform the functional definition of the product into the design of its structure, during the conceptual design phase. The developed example will concern the design of a bathroom scale.

2007-01-01

404

LC-MS characterization of metoclopramide photolysis products

British Library Electronic Table of Contents (United Kingdom)

The aim of this study was to investigate the photodegradation of the antiemetic drug metoclopramide. Metoclopramide aqueous solutions were photoirradiated at 254nm under argon atmosphere. Irradiated metoclopramide solutions were analyzed by high performance liquid chromatography-ion trap mass spectrometry in order to characterize photolysis products. Rapid decrease in metoclopramide purity, following first-order kinetic, was observed following irradiation. The structures of 18 photolysis products were tentatively identified based on their mass spectra and fragmentation. The main degradation mechanism was scission of the chlorine which could be followed by polymerization of the resulting products since dimeric and trimeric products were observed.

2009-01-01

405

Eco-profiling Inco Limited production : a case study

Basic studies on design supporting system of offshore structures; Kaiyo kozobutsu wo taisho toshita sekkei shien system ni kansuru kisoteki kenkyu

Inco Ltd., a primary producer of nickel and an important producer of copper, cobalt, precious metals and sulphur products, initiated a project in 1997 to conduct eco-profiles of its worldwide metal production in an effort to improve its internal processing, marketing and environmental management. The study made it possible to recognize some internal and external benefits including the opportunity to gain operating efficiencies and improved process/product design, the development of better environmental performance indicators to guide decision making, and the availability of comprehensive product related environmental data to meet the informational needs of customers, designers, engineers and governments. This paper described the life cycle assessment (LCA) process used by Inco to conduct the study. A life cycle inventory (LCI) was used to quantitatively calculate resource requirements and environmental ...

2000-07-01

406

Improving information technology to maximize fenestration energyefficiency

Authors already reported the integrated system so called SODAS (System Of Design and Assembling for Shipbuilding) which was based on product model concept. And products in shipbuilding industry today are not limited to ship structures alone. Therefore, it is desirable that a more global product model which can describe another products such as offshore structures should be considered. In this paper, a product model of offshore structures is discussed. Compared with the case of ship structures, the distinctive feature in the design stage of offshore structures lies in the initial design stage. Thus in this paper, some of the new model in initial design stage are introduced. Some of them are `Function Element Model` which expresses functional concept by designers. In the other design stage, product information such as `Room` and `Part` which were already ...

1995-12-31

407

Using of Itaipu hydroelectric power plant-Brazil-electric power surplus for ammonia synthesis in nitrogen fertilizers production; Utilizacao da energia junto a Usina Hidreletrica de Itaipu para a sintese de amonia para fertilizantes nitrogenados

Improving software for the analysis of fenestration product energy efficiency and developing related information technology products that aid in optimizing the use of fenestration products for energy efficiency are essential steps toward ensuring that more efficient products are developed and that existing and emerging products are utilized in the applications where they will produce the greatest energy savings. Given the diversity of building types and designs and the climates in the U.S., no one fenestration product or set of properties is optimal for all applications. Future tools and procedures to analyze fenestration product energy efficiency will need to both accurately analyze fenestration product performance under a specific set of conditions and to look at whole fenestration product energy ...

2001-06-06

408

Potential of production of biodiesel starting from the chicken oil in the cooperatives of the West of Parana, Brazil; Potencial de producao de biodiesel a partir do oleo de frango nas cooperativas do oeste do Parana

Secondary energy can be described as a surplus of electrical energy in a hydropower because the lower demand of energy in some period of the day and the excess of water in the reservoir during the rainy periods. This study proposes the using of the excess of energy at Itaipu Bi nacional for electrolytically hydrogen production and ammonia for nitrogenous fertilizers. Ammonia is a raw material for nitrogenous fertilizers synthesis and produced using hydrogen and nitrogen from the atmosphere. This paper determines the minimal cost of hydrogen production and the capacity of hydrogen production, with this data and according the ammonia market for nitrogenous fertilizers in the Centre/South region was estimated the better capacity of ammonia production for one plant near Itaipu. The process of ammonia production using energy excess is the most environmental acceptable. (author)

2000-07-01

409

Optimization of mechanical properties of recycled plastic products via optimal processing parameters using the Taguchi method

Brazil occupies a prominence place in the development and use of sources renovate of energy, due to its great territorial extension, climate and several alternatives. One of these it is the bio diesel,o production which can substitute the oil diesel, decreasing the impacts to the environment. In the productive chain of chicken meat a residue, chicken oil, is generated with potential for biodiesel production. In this work they were certain characteristics physical chemistries, that can influence in the reactions of transesterification of the chicken oil. It was lifted up the potential of production of chicken oil in the cooperatives of the area west of the state of Parana and yield in biodiesel. The bio diesel production by cooperatives could be of 19.525.209,0 kg/year of bio diesel and the yield of 95%. (author)

2006-07-01

410

British Library Electronic Table of Contents (United Kingdom)

The large amount of plastic products presently produced necessitates recycling and reuse of these non-biodegradable materials. However, the degradation in the mechanical properties of products made from recycled plastic is a major drawback that limits their use. This study aims to improve the mechanical properties of products made from recycled plastic by utilizing the Taguchi optimization method, instead of coupling the products with additives. By adopting L9 Taguchi OA, products made from various compositions of virgin and recycled plastic are produced by injection moulding. Four controllable factors (i.e., melt temperature, packing pressure, injection time, and packing time), each at three levels, are tested to determine the optimal combination of factors and levels in the manufacturing...

2011-01-01

411

Fragrance ingredient labelling in products on sale in the U.K.

British Library Electronic Table of Contents (United Kingdom)

Summary Background The seventh amendment of the European Union (EU) Cosmetics Directive (March 2005) and the Detergents Regulations of the EU (October 2005) are now legal requirements in Europe. Cosmetic products and detergents must be labelled for 26 individual named fragrances, when present at concentrations of > 10 parts per million (p.p.m.) in leave-on products and > 100 p.p.m. in rinse-off products. Objectives To make an assessment of the exposure pattern to fragrance of the U.K. consumer and to determine the frequency with which the constituent fragrances of fragrance mix I (FM I) and fragrance mix II (FM II) are included in products currently sold in the U.K. Methods A study of perfumed cosmetic and household products available on the shelves of U.K. retailers was carried out in Jan...

2007-01-01

412

Effect of alleviating products with different mode of action on physiology and yield of olive under drought

British Library Electronic Table of Contents (United Kingdom)

Two years old self-rooted Koroneiki olive trees (Olea europaea L.) were subjected to two irrigation regimes, i.e. the fully irrigated and the severely water stressed trees, while they were treated with three alleviating products of different mode of action. The products used were the osmolyte glycine betaine, the antioxidant Ambiol and the heat and irradiance reflecting kaolin clay particles. The effects of product application and water regime on leaf characteristics, shoot and root growth, photosynthesis, leaf compatible solids (carbohydrates) concentration and yield were evaluated. All products applied, exhibited significant alleviating action, based on the relative alleviation index. Irrigated trees exhibited greater growth than drought stressed ones, while the ameliorating products mai...

2010-01-01

413

Cumulative production of direct photons and leptonic pairs

Applying Industrial Design and Cost Engineering to New Product Development in Early-Stage Firms

Leptonic pair production on nuclei ad EMC-effect are discussed within the frames of the flucton model with scaling distortion. Cumulative production of direct photons and leptonic pairs as a test of the model of hard collisions is considered. The results of calculations of massive leptonic pair production cross sections on nucleus fluctons, caculations of the ratio of deuterium and iron structural functions, cross sections of direct photon production on sup(181)Ta nuclei at Esub(p)=400 GeV and cross section of #pi#-meson production in the model of hard collisions are presented. Experimental discovery of direct cumulative photons is concluded to be important for understanding the mechanism of parton hard scattering from nucleus fluctons.

1984-06-19

414

British Library Electronic Table of Contents (United Kingdom)

For early-stage firms, successful commercialization of each new product is critically important, given the shortage of financial resources, the limited product portfolio, and small staffs typical of such firms. This paper investigates two key contributing factors for new product success in entrepreneurial firms: designing products that are appealing to target users in both form and function and designing products that can be manufactured at an attractive margin so that the new enterprise can generate much needed positive cash flow. These two practices-industrial design and cost engineering-are well studied in the context of larger, established corporations but have not been explored in the context of new ventures. This study focuses on the intensity of individual and combined adoption of d...

2011-01-01

415

Amorphous cellulose gel as a fat substitute in fermented sausages

British Library Electronic Table of Contents (United Kingdom)

Fermented sausages were produced with 25%, 50%, 75% or 100% of their pork back fat content replaced by amorphous cellulose gel. The sausage production was monitored with physical, chemical and microbiological analyses. The final products were submitted to a consumer study, and the volatile compounds of the final products were extracted by solid-phase microextraction and analyzed by GC/MS. The reformulated fermented sausages had significant reductions in fat and cholesterol, and the volatile compounds derived from lipid oxidation were also reduced in the final products. These results suggest that the substitution of up to 50% of the pork back fat content by amorphous cellulose gel can be accomplished without a loss of product quality, enabling the production of fermented sausages with the l...

2012-01-01

416

an expert system for estimating forest harvesting productivity and cost

What Do We Feed to Food-Production Animals? A Review of Animal Feed Ingredients and Their Potential Impacts on Human Health

Sep 1, 2011 ... Title: THINEX - an expert system for estimating forest harvesting productivity and cost. Author: LeDoux, C. B.; Gopalakrishnan, B.; Pabba, R. S. ...

417

ObjectiveAnimal feeding practices in the United States have changed considerably over the past century. As large-scale, concentrated production methods have become the predominant...Full Text Available

2007-05-01

418

Waste to value

Towards Commercial Production of Sponge Medicines

The basic technology behind bioethanol production is tried and tested. Alcohol is produced through the fermentation of sugar, which when processed can be used as a biofuel. Now, however, a multitude of new technologies are set to also make cellulose available as a raw material for ethanol production. (orig.)

2010-07-01

419

Top quark physics: Summary

Sponges can provide potential drugs against many major world-wide occurring diseases. Despite the high potential of sponge derived drugs no sustainable production method has been developed. Thus far...Full Text Available

420

The associations between milk production, milk composition and Salmonella in the bulk milk supplies of dairy farms in Ontario.

This talk summarizes recent progress in top quark physics studies for high energy linear electron-positron colliders as presented at the LCWS2000 Workshop at Fermilab. New results were presented for top pair production at threshold and in the continuum, as well as for top production at #gamma##gamma# colliders.

2001-07-09

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Supply of medium and small hydropower equipment

The purpose of this study was to assess changes in dairy herd milk production and milk composition associated with changes in Salmonella contamination of bulk milk on dairy farms in southwestern Ontario....Full Text Available

1989-04-01

422

Single dilepton production at [ital e][sup +][ital e][sup [minus

This paper presents a brief account of Lingling Hydroelectric Power Equipment General Works, the capacity of equipment production, the organization body, the quality assurance system and supervision of government department on its product quality.

1995-07-01

423

Radionuclide X-ray fluorescence analysis of waste water from the production of citric acid

We calculate the cross sections for the single production of doubly charged dileptons, both scalar and vector, at [ital e][sup +][ital e][sup [minus

1994-08-01

424

Radionuclide X-ray fluorescence analysis of waste water from the production of citric acid

Radionuclide X-ray fluorescence analysis was used for the determination of iron, calcium, potassium, copper, manganese and zinc in waste water from the production process of citric acid.

1982-02-18

425

RXPERT: A Decision Support Expert System for Drug Product Interchangeability Assessment

Radionuclide X-ray fluorescence analysis was used for the determination of iron, calcium, potassium, copper, manganese and zinc in waste water from the production process of citric acid. (author).

1982-02-01

426

Quality assurance requirements for high-level waste form production

A knowledge-based expert system has been developed to support human decision makers who assess drug products for inclusion in the Saskatchewan Formulary. Formulary inclusion and deletion decisions...Full Text Available

1989-11-08

427

Production, Purification, and Characterization of ?-Galactosidase from Monascus pilosus

This paper discusses the quality assurance requirements for high-level canistered waste form products which would be acceptable for disposal in a deep geologic repository. A review plan and guidelines are provided.

1989-04-02

428

Production of three vector bosons in #gamma# #gamma# colliders

A Monascus pilosus strain was selected for production of intracellular α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Galactose...Full Text Available

1986-11-01

429

Production of dissolved DNA, RNA, and protein by microbial populations in a Florida reservoir.

The production of three vector bosons in #gamma# #gamma# collisions studying the reactions #gamma# + #gamma# #-># W"+ + W"- + Z"0 and #gamma# + #gamma# #-># W"+ + W"- + #gamma#. 12 refs., 4 figs., 2 tabs.

430

Optimization of sorghum-methane production systems

Production of dissolved macromolecules by ambient autotrophic and heterotrophic microbial populations was measured in a eutrophic Florida reservoir by in situ labeling with various radioactive substrates....Full Text Available

1990-10-01

431

Normal human B lymphocytes and mononuclear cells respond to the mitogenic and cytokine-stimulatory activities of Borrelia burgdorferi and its lipoprotein OspA.

This dissertation concerns a study of the utilization of sorghum biomass as the fuel for a methane production system. The objective of this research is to determine an optimal sorghum-methane production system based on anaerobic batch digestion technology. The system is described as the interaction of three components: estimation of available biomass, storage of biomass, and the gas production. Levelized costs are obtained for each component. The biomass supply is assumed to decay exponentially in quality with storage time. The gas production process is a batch type of digestion with an exponential production rate. Gas production is formulated for a fixed planning horizon (one year) as a function of the number of batches and the batch residence times. The cost equation is then described as a function of these gas production equations and ...

1984-01-01

432

Nitric Oxide and Nitrous Oxide Production by Soybean and Winged Bean during the in Vivo Nitrate Reductase Assay ¹

Borrelia burgdorferi produces potent cell-activating molecules capable of stimulating polyclonal proliferation and immunoglobulin production by murine B lymphocytes and cytokine production by a variety...Full Text Available

1994-02-01

433

National Newborn Screening Status Reports

This study was conducted to determine by gas chromatography (GC) and mass spectrometry (MS) the identity and the quantity of volatile N products produced during the helium-purged in vivo...Full Text Available

1986-11-01

434

Multiple Epoxide Hydrolases in Alternaria alternata f. sp. lycopersici and Their Relationship to Medium Composition and Host-Specific Toxin Production

... detected (and reported) as a by-product of MRM screening (MS/MS) targeted by Law or Rule ... detected (and reported) as a by-product of MRM screening (MS/MS) targeted by Law or Rule ...

435

Molecular breeding of cereals for aluminium resistance

The production of Alternaria alternata f. sp. lycopersici host-specific toxins (AAL toxins) and epoxide hydrolase (EH) activity were studied during the growth of this...Full Text Available

1999-06-01

436

Microsoft Word - 210711_Santos_Second_Quarter_Activities_Report

Aluminium (Al3+) toxicity is the primary factor limiting crop production on acidic soils worldwide. In addition to an application of lime for soil amelioration, Al3+ resistant plant varieties have been deployed to raise productivity on such hostile soils. This has been possible due to the exploita...

437

Mapping Quantitative Trait Loci Controlling Milk Production in Dairy Cattle by Exploiting Progeny Testing

Item Production (mmboe) Production costs ($m) Depreciation, depletion & amortisation (DD&A) expense ($/boe) ...3 1351.4 1190.7103.8 107.84110.79139.6

438

Labeled Products and Our Partners | Design for the Environment...

We have exploited ``progeny testing'' to map quantitative trait loci (QTL) underlying the genetic variation of milk production in a selected dairy cattle population. A total of 1,518 sires, with progeny...Full Text Available

1995-02-01

439

In Silico Atomic Tracing by Substrate-Product Relationships in Escherichia coli Intermediary Metabolism

choice Charlotte NC *ecosential for smooth cooktops 2010 Jelmar, LLC Skokie IL CLR Cooktop Cleaner 2004 LotusLand Products, LLC Dallas TX *Believe -- BeSpicy Oven and Grill...

2011-08-18

440

Immune activation and IL-12 production during acute/early HIV infection in the absence and presence of highly active, antiretroviral therapy

We present a software system that computationally reproduces biochemical radioisotope-tracer experiments. It consists of three main components: A mapping database of substrate-product atomic correspondents...Full Text Available

2003-11-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Health hazards in the production and processing of some fibers, resins, and plastics in Bulgaria.

Suppressed IL-12 production and maladaptive immune activation, both of which are ameliorated by successful highly active antiretroviral therapy (HAART), are thought to play important roles in the immunopathogenesis...Full Text Available

2008-12-01

442

Glucosinolate breakdown products as insect fumigants and their effect on carbon dioxide emission of insects

Results of the toxicological studies of working conditions, general and professional morbidity, and complex examinations carried out on workers engaged in the production of polyamides, polyacrylonitrile...Full Text Available

1976-10-01

443

BackgroundGlucosinolate breakdown products are volatile, therefore good candidates for insect fumigants. However, although they are insecticidal, the mode of action of such natural...Full Text Available

444

Geothermal status report

Flucton model with scaling breaking: EMC effect and lepton pair production on nuclei

This article examines the effects of competition of geothermal energy production with other technologies. The topics of the article include near-term market growth, cause for cautious optimism, limits to development of geothermal energy production, economic arguments for development of geothermal power plants, the effects of a competitive market on industry survival.

445

Fermentation of Barley by Using Saccharomyces cerevisiae: Examination of Barley as a Feedstock for Bioethanol Production and Value-Added Products ?

The EMS effect is explained in the flucton model as a consequence of scale invariance violation. Nontrivial behaviour of the ratio between structural functions and production cross sections for lepton pairs for different nuclei at x > 1 is predicted.

446

Exposure to dioxin and nonneoplastic mortality in the expanded IARC international cohort study of phenoxy herbicide and chlorophenol production workers and sprayers.

The objective of this study was to examine the ethanol yield potential of three barley varieties (Xena, Bold, and Fibar) in comparison to two benchmarks, corn and wheat. Very high gravity (VHG; 30%...Full Text Available

2009-03-01

447

Estimating production rates and operating costs of timber harvesting equipment in the northern Rockies. General technical report

The authors studied noncancer mortality among phenoxyacid herbicide and chlorophenol production workers and sprayers included in an international study comprising 36 cohorts from 12 countries followed...Full Text Available

1998-04-01

448

Epicuticular Wax Accumulation and Fatty Acid Elongation Activities Are Induced during Leaf Development of Leeks1

The report summarizes studies of ground, cable, and aerial logging systems in the Northern Rockies over a 15-year period. It provides nomographs and tables for calculating productivity and a system for comparing energy requirements.

1982-09-01

449

Effects of bovine leukemia virus infection on production and reproduction in dairy cattle.

Epicuticular wax production was evaluated along the length of expanding leek (Allium porrum L.) leaves to gain insight into the regulation of wax production. Leaf segments from the...Full Text Available

1998-03-01

450

Development Document for Proposed Effluent Limitations Guidelines and Standards for the Concentrated Aquatic Animal Production Industry Point Source Category.

The purpose of this study was to determine the effects of bovine leukemia virus (BLV) infection on production, reproduction and longevity in dairy cattle. The study population was a commercial Holstein...Full Text Available

1992-10-01

451

Determining insoluble impurities in spent sulfuric acid from the production of synthetic alcohols

This section presents background information supporting the development of effluent limitations guidelines and standards for the concentrated aquatic animal production (CAAP) point source category. Section 1.1 presents the legal authority to regulate the ...

2002-01-01

452

Deletion of the Receptor for Advanced Glycation End Products Reduces Glomerulosclerosis and Preserves Renal Function in the Diabetic OVE26 Mouse

Ammonium sulfate is produced from spent sulfuric acid from the production of alcohols. Certain mineral and organic impurities exist in the sulfuric acid that impair the quality of the ammonium sulfate. A detailed method for the chemical analysis for the impurities is presented.

1983-01-01

453

Decoding of Matrix-Product Codes

OBJECTIVEPrevious studies showed that genetic deletion or pharmacological blockade of the receptor for advanced glycation end products (RAGE) prevents the early structural changes...Full Text Available

2010-08-01

454

Cost-Effectiveness Analysis of Proposed Effluent Limitations Guidelines and Standards for the Metal Products and Machinery Industry (Phase 1).

We propose a decoding algorithm for the $(u\\mid u+v)$-construction that decodes up to half of the minimum distance of the linear code. We extend this algorithm for a class of matrix-product codes in two different ways. In some cases, one can decode beyond the error correction capability of the code.

2011-01-01

455

Catalyst for olefin production

This cost-effectiveness analysis supports the proposed effluent limitations guidelines and standards for the Metal Products and Machinery Industry (MP and M) Phase I Industry. The report assesses the cost-effectiveness of five regulatory options for indir...

1995-01-01

456

Carboxymethylation of peat in 2-propanol

A process is claimed for selectively preparing alpha-olefins having from 2 to about 22 carbon atoms by contacting a gaseous mixture containing carbon monoxide and hydrogen with an iron titanate alkali metal hydroxide catalyst at reaction conditions correlated so as to favor the formation of a substantial proportion of such alpha-olefin product.

1981-04-14

457

British Library Electronic Table of Contents (United Kingdom)

The effects of the time and temperature of alkaline pretreatment and the time and temperature of the carboxymethylation of peat in 2-propanol on the concentration of carboxymethyl groups and the solubility of the resulting products in water and an aqueous alkali solution were studied. The viscosity characteristics of the aqueous and aqueous alkaline solutions of peat carboxymethylation products were studied.

2011-01-01

458

CTX-M ?-Lactamase Production and Virulence of Escherichia coli K1

Auxin-Induced Ethylene Production as Related to Auxin Metabolism in Leaf Discs of Tobacco and Sugar Beet 1

We report a patient with neonatal meningitis caused by a CTX-M-1–producing Escherichia coli K1 strain. The influence of CTX-M production on virulence was investigated in cell...Full Text Available

2009-12-01

459

Associations between dairy production indices and lipoarabinomannan enzyme-immunoassay results for paratuberculosis.

Exogenously supplied indole-3-acetic acid (IAA) stimulated ethylene production in tobacco (Nicotiana glauca) leaf discs but not in those of sugar beet (Beta vulgaris...Full Text Available

1983-11-01

460

Analysis of data on cumulative antiproton production by 10-GeV protons

Data from an epidemiological study in Ontario, involving 304 dairy herds, were used to identify associations between selected production indices and lipoarabinomannan antigen serological test results...Full Text Available

1991-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Alkyl acrylate adducts of polyamines, ether amines and ether polyamines

The data are analyzed on cumulative antiproton production on Be, Al, Cu, and Ta nuclei induced by 10 GeV protons. The analysis is carried out in the framework of the flucton quark fragmentation model.

462

Agricultural R&D, technology and productivity

Disclosed are additives for hydrocarbon fuels which are the reaction products of a polyamine and an alkyl ester of acrylic or alkyl acrylic acid. A preferred composition is a mixture of the reaction product and oxygenated or non-oxygenated mono- or polyamines.

1980-12-23

463

Advanced glycation end-products in senile diabetic and non-diabetic patients with cardiovascular complications

The relationships between basic and applied agricultural R&D, developed and developing country R&D and between R&D, extension, technology and productivity growth are outlined....Full Text Available

2010-09-27

464

Activation of peripheral blood mononuclear cells in bronchoalveolar lavage fluid from patients with sarcoidosis: visualisation of single cell activation products.

Advanced glycation end products (AGEs) have been reported to contribute to aging and cardiovascular complications. In the present study, the immunoreactivity of AGEs in human serum samples of healthy...Full Text Available

2008-12-01

465

Acetylsalicylic acid as soluble tablets

BACKGROUND--Interstitial lung diseases are characterised by the recruitment of mononuclear cells to disease sites where maturation occurs and activation products, including lysozyme (LZM), are released....Full Text Available

1994-11-01

466

Access Intervention in an Integrated, Prepaid Group Practice: Effects on Primary Care Physician Productivity

Realization of Pharmacological Tests, Development of the Specifications and Technical Documentation and Registration in the Russian Federation of Medical Products as Soluble Tablets of Acetylsalicylic Acid/Calcium Carbonate in Two Dosages Intended for use as Medical Products Smoothing, Febrifugal and Antiaggregation Action and Development of Technology of Tableting the Above-Stated Preparations

467

A field study on the effects of dietary monensin on milk production and milk composition in dairy cows

ObjectiveTo estimate the joint effect of a multifaceted access intervention on primary care physician (PCP) productivity in a large, integrated prepaid group practice.Data...Full Text Available

2008-10-01

468

A Multi-Worksite Analysis of the Relationships among Body Mass Index, Medical Utilization and Worker Productivity

The objectives of this study were to quantify the effect of 16 ppm of dietary monensin on milk production and composition of dairy cows, and to investigate factors having a potential impact on this...Full Text Available

2010-04-01

469

Waterstop repair products

BackgroundThe relationships between worker health and productivity are becoming clearer. However, few large scale studies have measured the direct and indirect cost...Full Text Available

2010-01-01

470

The impact of intensive and extensive rearing environment on mucosal immunity in the piglet (GUTWEAN)

Results of an evaluation of 18 different products that can be used as waterstops in hydraulic dams to minimize or eliminate leaks at construction joints, were discussed. The materials were tested for permeability and adherence at 23, 15 and 5 degrees C. A two-component polyurethane resin product was found to be the one that best fulfilled the evaluation criteria. It was also found that the successful injection of a borehole depends not only on selecting the product with the best performance characteristics, but also on the technique used. Hence the preparation of the resin and the pressure at which it is injected are important performance criteria. 5 refs., 6 photos, 4 figs.

1997-09-22

471

Environmental Research Database

DescriptionPig production represents an important source of revenue for the agricultural industry of the UK. However as a direct result of environmental and animal welfare concerns European-wide directives are now in place which severely restrict both the non-clinical use of production enhancers/chemotherapeutics and heavy metals in animal production and also prevent piglet weaning prior to 28 days of age. These legislative events have had a major impact on pig production within Europe rendering current pr [continued...

2009-01-31

472

Study of {eta}'-meson production in the reaction pd {yields} {sup 3}He {eta}' at COSY-11

Spray-formed tooling and aluminum strip

No abstract prepared.

2002-03-01

473

Scenario-Based Affordability Assessment Tool

Spray forming is an advanced materials processing technology that converts a bulk liquid metal to a near-net-shape solid by depositing atomized droplets onto a suitably shaped substrate. By combining rapid solidification processing with product shape control, spray forming can reduce manufacturing costs while improving product quality. De Laval nozzles offer an alternative method to the more conventional spray nozzle designs. Two applications are described: high-volume production of aluminum alloy strip, and the production of specialized tooling, such as injection molds and dies, for rapid prototyping.

1995-11-01

474

Ratio of the {lambda} / {sigma}{sup 0} hyperon production via the excitation of the N{sup *}(1650)S{sub 11} resonance

... Dynamics) environment (ref. 13) to work seamlessly with their flagship products, MSC.Nastran and MSC.Patran. In addition ...

2003-03-01

475

Provenance study of excavated pottery from Beirut using PIXE cluster analysis

No abstract prepared.

2002-03-01

476

Production of cumulative pions and kaons

The study of imported ceramics found in Beirut and suspected to belong to north Syrian products will provide key evidence for the roles in trade of the various postulated centers in northern Syria and northern Lebanon. It is proposed that a comprehensive range of likely north Syrian products imported to Beirut will be chemically analyzed by PIXE (proton induced X-ray emission) in order to characterize, compare and isolate the various regional production centers that supplied the city from the Persian to Byzantine periods. In this work, we were mainly focusing on the characterization of Amrit products, by using 3 MeV proton beam and Al funny filter, as X-ray absorber.

2006-08-01

477

Production Data Package 267 Gallon External Fuel Tank

#pi#"#+-# and K"#+-# cumulative production in pA #-># #pi#"#+-#+x and pA #-># K"#+-#+x reactions at 10.14 GeV proton beam energy on nuclei (Be-Ta) was invesigated. Measurements were conducted using FAS-1 focusing hadron spectrometer. Invariant cross sections of #pi#"#+-# and K"#+-# cumulative production, values of ratio of this cross sections, dependences of production cross sections for pions and kaons on cumulative number and mass number of nucleus target, as well as on transverse momentum are measured. Difference of cumulative meson spectra occurring in different nuclei fragmentation is discussed in terms of flucton model. 17 refs.; 14 figs.

1988-06-14

478

Product yield and hydrogen consumption selectivity tests for coal liquefaction catalyst development

... It is recommended that the drawings be validated in accordance with NAVAIRINST 4333.10 and the data package, Appendix A, be used for ...

1973-08-23

479

Problem Definition Studies on Potential Environmental ...

A method for analyzing the experimental results of coal liquefaction reactions which is applicable to a number of aspects of coal liquefaction research and process control, including rapid selectivity and performance screening for catalysts; correlation of laboratory results with process parameters; and optimization of product yield with plant process conditions is described here. Ternary diagrams of product/by-product distributions for the coal liquefaction using Co/Mo catalysts combined with a hyperbolic relationship for the conversion of various hydrocarbon fractions can be used for catalyst screening. A hydrogen consumption diagram used to provide a more significant selectivity test than the hyperbolic correlation is also included. (BLM)

1981-01-01

480

Preparation of "2"0"3Pb compounds for studies on pathway and effects of lead pollution

... mutagenic, while two potential environmental transformation products, 1,4- diaminoanthraq Ynone and 1,4-dihydroxyanthraquinone are strongly ...

1979-11-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Neutron production in the 120 MeV alpha particle bombardment of deuterium

... air pollution coprecipitation isotope production lead 203 proton beams

482

Neutron activation analysis of molybdenum in molybdenum concentrates and in the molybdenum reprocessing products

1971. 111 p. United States Chamberlin, EP Texas Agricultural and

483

Neutrino viscosity in Bianchi type IX universes

... activation analysis gamma spectra mev range molybdenum molybdenum ores

484

Liquid hydrocarbon fuel composition

The authors investigate the isotropization of anisotropic closed spaces and the entropy production due to neutrino viscosity. (Auth.).

485

JENDL-4.0: A database on neutron-induced reactions for nuclear science and engineering

A fuel composition comprising a liquid hydrocarbon fuel and a detergent amount of the product of reaction between a polyamine and a stearic acid is described.

1983-07-19

486

Inhibitory Effects of Methanolic Extracts of Medicinal Plants on Nitric Oxide Production in Activated Macrophage RAW 264.7 Cells

... compilation fission products j codes mixed oxide fuels neutron reactions

2010-12-01

487

Improvement of hEGF Production with Enhanced Cell Division Ability Using Dissolved Oxygen Responses to Pulse Addition of Tryptone

Full Text Available

2009-06-01

488

Evaluation and Experimental Production of Single-Phase Full-wave Rectification Type for X-ray Equipment of High Precision

Full Text Available

2009-02-01

489

Enhancement of Tissue Type Plasminogen Activator (tPA) Production from Recombinant CHO Cells by Low Electromagnetic Fields

Full Text Available

2011-01-01

490

Effects of Supplemental Medicinal Plants (Artemisia, Acanthopanax and Garlic) on Productive Performance of Sows and on Growth and Carcass Traits in Finishing Pigs

Full Text Available

2002-06-01

491

Full Text Available

2010-04-01

492

Connections

Biomass production in Florida

... many intriguing new possibilities in the materials sciences. Or consider solitons, a mathematical ... point out that advanced instrumentation, new materials, novel production methods, and efficient ...

493

Automated purification of Borrelia burgdorferi s.l. PCR products with KingFisher"T"M magnetic particle processor prior to genome sequencing

Florida posseses climatic, land, and water resources favorable for abundant biomass production. Therefore, a statewide program has been initiated to determine adapted species for the available array of production sites. Plant resources under investigation include woody, aquatic, grasses, hydrocarbon, and root crop species. The goal is to produce a continuous stream of biomass for the various biofuel conversion options. Preliminary yields from energy cropping experiments range from about 10 to nearly 90 metric tons per hectare per year, depending on the crop and the production systems employed. (Refs. 15).

1981-08-01

494

Application of low dose radiation for preservation of sea foods

Borrelia burgdorferi sensu lato genospecies were differentiated by PCR-based sequencing of the borrelial flagellin gene. To evaluate the usefulness of KingFisher"T"M magnetic particle processor in PCR product purification, borrelia PCR products were purified with KingFisher"T"M magnetic particle processor prior to cycle sequencing and the quality of the sequence data received was analyzed. KingFisher was found to offer a rapid and reliable alternative for borrelial PCR product purification.

495

An Investigation of the Ozone Production at the Process of the UV Photodecomposition of the Oxygen

Treatment of food with low doses of gamma radiation has been recognized to have two main advantages. These consist of: (1) improvement of food safety by elimination of pathogens and (2) reduction of microbial spoilage and extension of shelf life of perishable items by reducing the number of viable spoilage organisms. Studies during the last few decades have conclusively proved the beneficial effects of radiation with respect to fishery products. The three potential areas of application to fish products include: (i) radurization for shelf life extension (ii) radicidation to eliminate food borne pathogens in the products and (iii) radiation treatment to dried products to control insects.

1994-03-01

496

ASPECTS OF PERFORMANCE EVALUATION OF WATERJET ...

English 2000 p. 213-214 Poland Ivanov, VV Klopovsky, KS Popov,

1999-07-11

497

APPLICATION OF CERTAIN SHALE PRODUCTS AS ...

... Propulsive efficiency is equivalent to the product of thrust efficiency and the hull/waterjet interaction efficiency. ... t'waterjet pump Ktorque repeated ...

1967-10-01

498

A b s t r a c t Vo l u m e

... unlimited. It may be released to the Clearinghouse. DePartment of Comereforsae o te enga Page 2. EDITED TRANSLATION A ...

1968-10-22

499

/linkages/journal/ Volume 6 Number 8

deodorants and sportswear and silver in washing ma- chines, refrigerators, health care products,

500