identifies potential genes: Topics by WorldWideScience.org

Robust consensus clustering for identification of expressed genes linked to malignancy of human colorectal carcinoma

Comparative sociogenomics has the potential to provide important insights into how social behaviour evolved. We examined brain gene expression profiles of the primitively eusocial wasp Polistes...Full Text Available

2010-07-22

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Gene expression analysis identifies potential biomarkers of phenanthrene in human hepatocytes (HepG2)

Previous studies have been conducted in gene expression profiling to identify groups of genes that characterize the colorectal carcinoma disease. Despite the success of previous attempts to identify...Full Text Available

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British Library Electronic Table of Contents (United Kingdom)

Polycyclic aromatic hydrocarbons (PAHs) are ubiquious in the environment both as natural products and as environmental contaminants. Among PAHs, phenanthrene (PH) that is ubiquitously distributed throughout the environment was subjected in this study. Although environmental distribution and metabolism of PH have been well reported, there are only a few studies examined the expression of mRNA and their functions on PH-induced toxicity. A new paradigm in toxicity screening, toxicogenomic technology represents a useful approach for evaluating the toxic properties of environmental pollutants. In this respect, we elicited the genes which were changed more than 2-fold by analysis of gene expression profiles in human hepatocellular carcinoma (HepG2) cells, exposed to PH by using human oligonucleo...

2011-01-01

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Chromosome mapping of human CDC25A and CDC25B phosphatases

Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection.

The human CDC25 tyrosine phosphatases trigger activation of CDC2 by removing inhibitory phosphates; thus the genes encoding these phosphatases may be suspected as potential oncogenes due to their role in promoting cell division. To date, three human CDC25 genes have been identified: CDC25A, B, and C. This communication describes the mapping of CDC25A to chromosome 3p21 and CDC25B to chromosome 20p13 by fluorescence in situ hybridization with confirmation by the polymerase chain reaction of hamster-human somatic cell hybrid DNA. 3p21 is near an area frequently involved in karyotypic abnormalities in renal carcinomas, small cell carcinomas of the lung, and benign tumors of the salivary gland. 20p13 does not seem to be a common area for karyotypic alteration in tumors. Mapping of these genes to their chromosomal loci may help identify tumors with abnormal ...

1993-10-01

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The early phase change Gene in Maize

African bovine trypanosomiasis caused by Trypanosoma sp., is a major constraint on cattle productivity in sub-Saharan Africa. Some African Bos taurus breeds are highly tolerant of infection, but the potentially more productive Bos indicus zebu breeds are much more susceptible. Zebu cattle are well adapted for plowing and haulage, and increasing their tolerance of trypanosomiasis could have a major impact on crop cultivation as well as dairy and beef production. We used three strategies to obtain short lists of candidate genes within QTL that were previously shown to regulate response to infection. We analyzed the transcriptomes of trypanotolerant N'Dama and susceptible Boran cattle after infection with Trypanosoma congolense. We sequenced EST libraries from these two breeds to identify polymorphisms that might underlie previously identified quantitative trait loci (QTL), and we assessed QTL regions and ...

2011-05-18

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Mutations in RNA Binding Protein Gene Cause Familial Dilated Cardiomyopathy

Recessive mutations of the early phase change (epc) gene in maize affect several aspects of plant development. These mutations were identified initially because of...Full Text Available

2002-01-01

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Gene-gene and gene-environment interactions: new insights into the prevention, detection and management of coronary artery disease

ObjectivesWe sought to identify a novel gene for dilated cardiomyopathy (DCM).BackgroundDCM is a heritable, genetically...Full Text Available

2009-09-01

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Glial inflammation and neurodegeneration induced by candoxin, a novel neurotoxin from Bungarus candidus venom: global gene expression analysis using microarray.

Despite the recent success of genome-wide association studies (GWASs) in identifying loci consistently associated with coronary artery disease (CAD), a large proportion of the genetic components of...Full Text Available

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The retinoic acid receptor beta (Rarb) region of Mmu14 is associated with prion disease incubation time in mouse.

Candoxin (PDB #1JGK), a three-finger neurotoxin from Bungarus candidus venom, inhibits post-synaptic neuromuscular and neuronal alpha7nACh-receptors, and induces delayed cell-death throughout the glial population. When applied to cultured human glial cell lines, candoxin (CDX) induced cell death in a concentration (EC(50) approximately 1muM) and time dependent manner. Results of TUNEL-histochemistry further confirm CDX-induced brain (hippocampus, frontal cortex, and temporal regions) damage when administered intracerebroventricularly (i.c.v) in adult mice. In this study, we explored differential gene expression profiles following exposure of human glial (Hs 683) cell lines to CDX at various time intervals using Affymetrix-GeneChips. By means of MAS and GeneSpring analyses, 105 genes whose expression was significantly (P<0.01) altered by at least 3-fold were selected. Results of the genome analysis ...

2005-11-23

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Histone deacetylase inhibitors and transforming growth factor-b induce 15-hydroxyprostaglandin dehydrogenase expression in human lung adenocarcinoma cells

In neurodegenerative conditions such as Alzheimer's and prion disease it has been shown that host genetic background can have a significant effect on susceptibility. Indeed, human genome-wide association studies (GWAS) have implicated several candidate genes. Understanding such genetic susceptibility is relevant to risks of developing variant CJD (vCJD) in populations exposed to bovine spongiform encephalopathy (BSE) and understanding mechanisms of neurodegeneration. In mice, aspects of prion disease susceptibility can be modelled by examining the incubation period following experimental inoculation. Quantitative trait linkage studies have already identified multiple candidate genes; however, it is also possible to take an individual candidate gene approach. Rarb and Stmn2 were selected as candidates based on the known association with vCJD. Because of the increasing overlap described between prion and ...

2010-12-06

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British Library Electronic Table of Contents (United Kingdom)

Histone deacetylase (HDAC) inhibitors have been actively exploited as potential anticancer agents. To identify gene targets of HDAC inhibitors, we found that HDAC inhibitors such as sodium butyrate, scriptaid, apicidin and oxamflatin induced the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), a potential cyclooxygenase-2 (COX-2) antagonist and tumor suppressor, in a time and concentration dependent manner in A549 and H1435 lung adenocarcinoma cells. Detailed analyses indicated that HDAC inhibitors activated the 15-PGDH promoter-luciferase reporter construct in transfected A549 cells. A representative HDAC inhibitor, scriptaid, and its negative structural analog control, nullscript, were further evaluated at the chromatin level. Scriptaid but not nullscript induced a signific...

2006-01-01

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Isolation of fetal DNA from nucleated erythrocytes in maternal blood

Assignment of the human aggrecan gene (AGC1) to 15q26 using fluorescence in situ hybridization analysis

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. The authors used monoclonal antibody against the transferrin receptor (TIR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, they were successful in ...

1990-05-01

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Using Regulatory and Epistatic Networks to Extend the Findings of a Genome Scan: Identifying the Gene Drivers of Pigmentation in Merino Sheep

The large aggregating proteoglycan aggrecan is a major structural component of the extracellular matrix of articular cartilage. Recent cDNA cloning of the human aggrecan gene (AGC1) reveals a core protein of at least 2316 amino acids characterized by several distinct structural domains. Two globular domains, termed G1 and G2, are present at the amino terminus of the molecule and a third, termed G3, is present at the carboxy terminus. The G1 domain is homologous in structure to the cartilage link protein and accounts for the aggregating potential of aggrecan through its ability to interact with hyaluronic acid. The aggrecan gene is known to consist of 15 exons, with each exon encoding a distinct functional region of the mature protein. However, while the link protein gene is known to reside on chromosome 5 in the human, the location of the aggrecan gene is currently undetermined in ...

1993-05-01

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Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression

Extending genome wide association analysis by the inclusion of gene expression data may assist in the dissection of complex traits. We examined piebald, a pigmentation phenotype in both human and Merino...Full Text Available

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Identification of cell cycle-related regulatory motifs using a kernel canonical correlation analysis

Noncoding RNAs play important roles in various aspects of gene regulation. We have identified 7SK RNA to be enriched in nuclear speckles or interchromatin granule clusters (IGCs), a subnuclear domain...Full Text Available

2010-12-01

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An integrative multi-dimensional genetic and epigenetic strategy to identify aberrant genes and pathways in cancer

BackgroundGene regulation is a key mechanism in higher eukaryotic cellular processes. One of the major challenges in gene regulation studies is to identify regulators affecting the...Full Text Available

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A polyacetylene compound from herbal medicine regulates genes associated with thrombosis in endothelial cells

BackgroundGenomics has substantially changed our approach to cancer research. Gene expression profiling, for example, has been utilized to delineate subtypes of cancer, and facilitated...Full Text Available

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Multilevel support vector regression analysis to identify condition-specific regulatory networks

From Toki-shakuyaku-san, an herbal formulation for “cleansing stagnated blood,” a key gene regulatory compound was purified and identified through a screening based on DNA microarray...Full Text Available

2007-12-15

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Large-scale cross-species oncogenomics identifies candidate oncogenes and tumor suppressor genes

Motivation: The identification of gene regulatory modules is an important yet challenging problem in computational biology. While many computational methods have been proposed to identify...Full Text Available

2010-06-01

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An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer

While genomic alterations identified in human tumors using techniques such as comparative genomic hybridisation (CGH) may be recurrent, they frequently encompass large regions, in some cases...Full Text Available

2010-02-01

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Transcriptome Profiling of a Toxic Dinoflagellate Reveals a Gene-Rich Protist and a Potential Impact on Gene Expression Due to Bacterial Presence

Cancers are highly heterogeneous and contain many passenger and driver mutations. To functionally identify tumor suppressor genes relevant to human cancer, we compiled pools of short harpin...Full Text Available

2008-11-28

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Linking Gene Expression and Functional Network Data in Human Heart Failure

BackgroundDinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear...Full Text Available

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A supervised approach for predicting patient survival with gene expression data

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

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SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

Rapid development in genomics in recent years has allowed the simultaneous measurement of the expression levels of thousands of genes using DNA microarrays. This has offered tremendous potential...Full Text Available

2010-01-01

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Molecular cloning of the N-terminus of GTBP

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

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Detecting exposure to environmental organic toxins in individual cells: towards development of a micro-fabricated device

Defects in mismatch repair genes cause the genetic instability characteristic of hereditary nonpolyposis colorectal cancer and a subset of sporadic colon tumors. The newest member of the mismatch repair gene family, GTBP, has recently been identified as a partial cDNA. Here, we describe the isolation of its 5{prime} terminus, allowing definition of the entire coding region. Several polymorphisms within the 5{prime} end were identified and are presented. 13 refs., 1 fig.

1996-02-01

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Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

A new method is being developed to quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000-400 cm-1). Distinct changes have been observed in the IR spectra after treatment of human cells in culture medium with PAHs and OCs. The potential use of this method for detecting exposure to PAHs and OCs has been tested and compared to a reverse transcription polymerase chain reaction (RT-PCR) assay that ...

1999-01-10

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British Library Electronic Table of Contents (United Kingdom)

To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-...

2011-01-01

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A unique horizontal gene transfer event has provided the octocoral mitochondrial genome with an active mismatch repair gene that has potential for an unusual self-contained function

Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution

BackgroundThe mitochondrial genome of the Octocorallia has several characteristics atypical for metazoans, including a novel gene suggested to function in DNA repair. This mtMutS...Full Text Available

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A systematic RNAi screen reveals involvement of endocytic pathway in neuronal dysfunction in a-synuclein transgenic C. elegans

The DNA sequence motif ATTTGCAT (octamer) or its inverse complement has been identified as an evolutionarily conserved element in the promoter region of immunoglobulin genes. Two major DNA-binding proteins that bind in a sequence-specific manner to the octamer DNA sequence have been identified in mammalian species--a ubiquitously expressed protein (Oct-1) and a lymphoid-specific protein (Oct-2). During characterization of the promoter region of the chicken immunoglobulin light chain gene, the authors identified two homologous octamer-binding proteins in chicken B cells. when the cloning of the human gene for Oct-2 revealed it to be a member of a distinct family of homeobox genes, they sought to determine if the human Oct-2 cDNA could be used to identify homologous chicken homeobox genes. Using a ...

1990-02-01

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British Library Electronic Table of Contents (United Kingdom)

Mutations or multiplications in a-synuclein gene cause familial forms of Parkinson disease or dementia with Lewy bodies (LB), and the deposition of wild-type a-synuclein as LB occurs as a hallmark lesion of these disorders, collectively referred to as synucleinopathies, implicating a-synuclein in the pathogenesis of synucleinopathy. To identify modifier genes of a-synuclein-induced neurotoxicity, we conducted an RNAi screen in transgenic C. elegans (Tg worms) that overexpress human a-synuclein in a pan-neuronal manner. To enhance the RNAi effect in neurons, we crossed a-synuclein Tg worms with an RNAi-enhanced mutant eri-1 strain. We tested RNAi of 1673 genes related to nervous system or synaptic functions, and identified 10 genes that, upon knockdown, caused severe growth/motor abnormalit...

2008-01-01

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Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in crohn's disease

British Library Electronic Table of Contents (United Kingdom)

AbstractBackground: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD). Methods: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25). Results: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) with...

2011-01-01

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Identification of three related human GRO genes encoding cytokine functions

Modulating factors in the expression of radiation-induced oncogenic transformation

The product of the human GRO gene is a cytokine with inflammatory and growth-regulatory properties; GRO is also called MGSA for melanoma growth-stimulatory activity. The authors have identified two additional genes, GRO#beta# and GRO#gamma#, that share 90% and 86% identity at the deduced amino acid level with the original GRO#alpha# isolate. One amino acid substitution of proline in GRO#alpha# by leucine in GRO#beta# and GRO#gamma# leads to a large predicted change in protein conformation. Significant differences also exist in the 3' untranslated region, including different numbers of ATTTA repeats associated with mRNA instability. A 122-base-pair region in the 3' region is conserved among the three GRO genes, and a part of it is also conserved in the Chinese hamster genome, suggesting a role in regulation. DNA hybridization with oligonucleotide probes and partial sequence analysis of the genomic clones ...

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Modulating factors in the expression of radiation-induced oncogenic transformation

Many assays for oncogenic transformation have been developed ranging from those in established rodent cell lines where morphological alteration is scored, to those in human cells growing in nude mice where tumor invasiveness is scored. In general, systems that are most quantitaive are also the least relevant in terms of human carcinogenesis and human risk estimation. The development of cell culture systems has made it possible to assess at the cellular level the oncogenic potential of a variety of chemical, physical and viral agents. Cell culture systems afford the opportunity to identify factors and conditions that may prevent or enhance cellular transformation by radiation and chemicals. Permissive and protective factors in radiation-induced transformation include thyroid hormone and the tumor promoter TPA that increase the transformation incidence for a given dose of radiation, and retinoids, selenium, vitamin E, and 5-aminobenzamide that ...

1990-08-01

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misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance

Many assays for oncogenic transformation have been developed ranging from those in established rodent cell lines where morphological alteration is scored, to those in human cells growing in nude mice where tumor invasiveness is scored. In general, systems that are most quantitaive are also the least relevant in terms of human carcinogenesis and human risk estimation. The development of cell culture systems has made it possible to assess at the cellular level the oncogenic potential of a variety of chemical, physical and viral agents. Cell culture systems afford the opportunity to identify factors and conditions that may prevent or enhance cellular transformation by radiation and chemicals. Permissive and protective factors in radiation-induced transformation include thyroid hormone and the tumor promoter TPA that increase the transformation incidence for a given dose of radiation, and retinoids, selenium, vitamin E, and 5-aminobenzamide that ...

1990-01-01

37

aHUS caused by complement dysregulation: new therapies on the horizon

Somite boundary formation is crucial for segmentation of vertebrate somites and vertebrae and skeletal muscle morphogenesis. Previously, we developed a Tol2 transposon-mediated...Full Text Available

2008-04-15

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The Arabidopsis ref2 Mutant Is Defective in the Gene Encoding CYP83A1 and Shows Both Phenylpropanoid and Glucosinolate Phenotypes

Atypical hemolytic uremic syndrome (aHUS) is a heterogeneous disease that is caused by defective complement regulation in over 50% of cases. Mutations have been identified in genes encoding both complement...Full Text Available

2011-01-01

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The Anopheles gambiae glutathione transferase supergene family: annotation, phylogeny and expression profiles

The Arabidopsis ref2 mutant was identified in a screen for plants having altered fluorescence under UV light. Characterization of the ref2 mutants showed that they...Full Text Available

2003-01-01

40

Reduced thermal sensitivity and Nav1.8 and TRPV1 channel expression in sensory neurons of aged mice

BackgroundTwenty-eight genes putatively encoding cytosolic glutathione transferases have been identified in the Anopheles gambiae genome. We manually annotated these...Full Text Available

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Phosphorylated PmrA Interacts with the Promoter Region of ugd in Salmonella enterica Serovar Typhimurium

Sensory neurons in aging mammals undergo changes in anatomy, physiology and gene expression that correlate with reduced sensory perception. In this study we compared young and aged mice to identify...Full Text Available

2006-06-01

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Osteopontin is elevated during neointima formation in rat arteries and is a novel component of human atherosclerotic plaques.

The Salmonella PmrA-PmrB system controls the expression of genes necessary for polymyxin B resistance. Four loci were previously identified as part of the regulon, and interaction of...Full Text Available

2000-07-01

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Oil palm (Elaeis guineensis Jacq.) tissue culture ESTs: Identifying genes associated with callogenesis and embryogenesis

In an earlier report, we used differential cloning to identify genes that might be critical in controlling arterial neointima formation (Giachelli, C., N. Bae, D. Lombardi, M. Majesky, and S. Schwartz....Full Text Available

1993-10-01

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Mutation Spectrum of EYS in Spanish Patients with Autosomal Recessive Retinitis Pigmentosa

BackgroundOil palm (Elaeis guineensis Jacq.) is one of the most important oil bearing crops in the world. However, genetic improvement of oil palm through conventional...Full Text Available

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Molecular cloning and nucleotide sequence of the beta-lytic protease gene from Achromobacter lyticus.

Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal dystrophies characterised ultimately by the loss of photoreceptor cells. We have recently identified a new gene (EYS) encoding...Full Text Available

2010-11-01

46

Identification of a Copper-Responsive Two-Component System on the Chromosome of Escherichia coli K-12

Two bacteriolytic enzymes secreted by Achromobacter lyticus M497-1 were purified and identified as being very similar (considering their amino acid composition and N-terminal sequence) to alpha- and...Full Text Available

1990-11-01

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Heritability of Measures of Kidney Disease Among Zuni Indians: The Zuni Kidney Project

Using a genetic screen we have identified two chromosomal genes, cusRS (ylcA ybcZ), from Escherichia coli K-12 that encode a two-component, signal...Full Text Available

2000-10-01

48

Genome-Wide Identification and Evolutionary Analysis of the Animal Specific ETS Transcription Factor Family

BackgroundThe long-term goal of the GKDZI (Genetics of Kidney Disease in Zuni Indians) Study is to identify genes, environmental factors, and genetic-environmental...Full Text Available

2010-08-01

49

DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase

The ETS proteins are a family of transcription factors (TFs) that regulate a variety of biological processes. We made genome-wide analyses to explore the classification of the ETS gene family. We identified...Full Text Available

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Comparative analysis of cis-regulation following stroke and seizures in subspaces of conserved eigensystems

CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA...Full Text Available

1999-03-16

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Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast

BackgroundIt is often desirable to separate effects of different regulators on gene expression, or to identify effects of the same regulator across several systems. Here, we focus...Full Text Available

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A new VCAN/versican splice acceptor site mutation in a French Wagner family associated with vascular and inflammatory ocular features

BackgroundHistone post-translational modifications are critical for gene expression and cell viability. A broad spectrum of histone lysine residues have been identified in yeast...Full Text Available

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A Cytogenetic Abnormality and Rare Coding Variants Identify ABCA13 as a Candidate Gene in Schizophrenia, Bipolar Disorder, and Depression

PurposeTo detail the highly variable ocular phenotypes of a French family affected with an autosomal dominantly inherited vitreoretinopathy and to identify the disease gene.MethodsSixteen...Full Text Available

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TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.

Schizophrenia and bipolar disorder are leading causes of morbidity across all populations, with heritability estimates of ∼80% indicating a substantial genetic component. Population genetics...Full Text Available

2009-12-11

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Targeted Capture and Next-Generation Sequencing Identifies C9orf75, Encoding Taperin, as the Mutated Gene in Nonsyndromic Deafness DFNB79

We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented...Full Text Available

1992-12-01

56

Repetitive satellite-like sequences are present within or upstream from 3 avian protein-coding genes.

Targeted genome capture combined with next-generation sequencing was used to analyze 2.9 Mb of the DFNB79 interval on chromosome 9q34.3, which includes 108 candidate genes. Genomic...Full Text Available

2010-03-12

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Regulation of cell proliferation and apoptosis in neuroblastoma cells by ccp1, a FGF2 downstream gene

Peculiar DNA sequences made up by the tandem repetition of a 5 bp unit have been identified within or upstream from three avian protein-coding genes. One sequence is located within an intron of the...Full Text Available

1983-03-11

58

Evolution of a cluster of innate immune genes (?-defensins) along the ancestral lines of chicken and zebra finch

BackgroundCoiled-coil domain containing 115 (Ccdc115) or coiled coil protein-1 (ccp1) was previously identified as a downstream gene of Fibroblast Growth Factor 2 (FGF2) highly expressed...Full Text Available

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Cloning of rice DNA and identification of tRNA gene clones

BackgroundAvian β-defensins (AvBDs) represent a group of innate immune genes with broad antimicrobial activity. Within the chicken genome, previous work identified 14 AvBDs...Full Text Available

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Analysis of the murine All-1 gene reveals conserved domains with human ALL-1 and identifies a motif shared with DNA methyltransferases.

DNA from 48 hr germinated rice embryos was cut with restriction endonuclease Bam H1 and cloned to the Bam H1 site on plasmid pBR 322. The clones containing recombinant DNA were selected by their sensitivity to tetracycline and resistance to ampicillin. Using /sup 32/P-labelled rice embryos tRNA as a probe two clones were identified to contain tRNA genes by colony hybridization.

1981-10-20

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Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy

A series of translocation break points found in a subset of human acute leukemias have one of the breaks on human chromosome 11q23. This region has recently been cloned and a large gene, ALL-1, with...Full Text Available

1993-07-01

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Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats

The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve ...

1994-03-01

63

British Library Electronic Table of Contents (United Kingdom)

To test the hypothesis that transduction of the channelrhodopsin-2 (ChR2) gene, a microbial-type rhodopsin gene, into retinal ganglion cells of genetically blind rats will restore functional vision, we recorded visually evoked potentials and tested the experimental rats for the presence of optomotor responses. The N-terminal fragment of the ChR2 gene was fused to the fluorescent protein Venus and inserted into an adeno-associated virus to make AAV2-ChR2V. AAV2-ChR2V was injected intravitreally into the eyes of 6-month-old dystrophic RCS (rdy/rdy) rats. Visual function was evaluated six weeks after the injection by recording visually evoked potentials (VEPs) and testing optomotor responses. The expression of ChR2V in the retina was investigated histologically. We found that VEPs could not b...

2010-01-01

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Sequences homologous to the human x- and y-borne zinc finger protein genes (ZFX/Y) are autosomal in monotreme mannals

beta-Lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands

The human zinc finger protein genes (ZFX/Y) were identified as a result of a systematic search for the testis-determining factor gene on the human Y chromosome. Although they play no direct role in sex determination, they are of particular interest because they are highly conserved among mammals, birds, and amphibians and because, in eutherian mammals at least, they have active alleles on both the X and the Y chromosomes outside the pseudoautosomal region. We used in situ hybridization to localize the homologues of the zinc finger protein gene to chromosome 1 of the Australian echidna and to an equivalent position on chromosomes 1 and 2 of the playtpus. The localization to platypus chromosome 1 was confirmed by Southern analysis of a Chinese hamster [times] platypus cell hybrid retaining most of platypus chromosome 1. This localization is consistent with the cytological homology of chromosome 1 between ...

1993-02-01

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Insulin-like growth factor binding protein-7 (IGFBP7) functions as a potential tumor suppressor in hepatocellular carcinoma (HCC).

Objectives: The purpose of this work was to study the genetic determinants responsible for extended-spectrum beta-lactamase (ESBL) resistance of Salmonella isolated from Dutch poultry, poultry meat and hospitalized humans. Methods: Thirty-four ESBL-resistant Salmonella isolates from The Netherlands were tested towards 21 antimicrobial agents. PCR and sequencing were used to determine the underlying genetic determinants responsible for the ESBL phenotypes. The transferability of the ESBL phenotypes was tested by conjugation to a susceptible Salmonella enterica serovar Dublin and plasmid purification, restriction fragment length polymorphism (RFLP) and pulsed-field gel electrophoresis (PFGE) were employed to further characterize a subset of the isolates. Results: A great genetic diversity was seen among the isolates. The bla(TEM-52) gene was most predominant and was found among Salmonella enterica serovars Blockley, Thomson, London, Enteritidis phage type 14b, ...

2005-01-01

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The DFNA5 gene, responsible for hearing loss and involved in cancer, encodes a novel apoptosis-inducing protein

PURPOSE: Hepatocellular carcinoma (HCC) is a highly virulent malignancy with no effective treatment thus requiring innovative and effective targeted therapies. The oncogene Astrocyte elevated gene-1 (AEG-1) plays a seminal role in hepatocarcinogenesis and profoundly downregulates insulin-like growth factor binding protein-7 (IGFBP7). The present study focuses on analyzing potential tumor suppressor functions of IGFBP7 in HCC and the relevance of IGFBP7 downregulation in mediating AEG-1 function.EXPERIMENTAL DESIGN: IGFBP7 expression was detected by immunohistochemistry in HCC tissue microarray and real-time PCR and ELISA in human HCC cell lines. Dual Fluorescence in situ hybridization was performed to detect loss of heterozygosity at IGFBP7 locus. Stable IGFBP7-overexpressing clones were established in the background of AEG-1-overexpressing human HCC cells and were analyzed for in vitro proliferation and senescence and in vivo tumorigenesis and ...

2011-09-16

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British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

2011-01-01

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Australian experience with herbicide tolerant (HT) and Bacillus thuringiensis (Bt) cotton

British Library Electronic Table of Contents (United Kingdom)

Australia?s nationally consistent framework for gene technology regulation is underpinned by the Gene Technology Act 2000, administered by an independent decision-maker, the Gene Technology Regulator. The object of the Act is ?to protect the health and safety of people, and to protect the environment, by identifying risks posed by or as a result of gene technology, and by managing those risks through regulating certain dealings with genetically modified organisms?. Marketing and trade impacts are outside the scope of assessments required by the Act. Since 2001, seven licences have been issued for the commercial cultivation of genetically modified (GM) cotton with insect resistance and/or herbicide tolerance. Licences have also been issued for 32 GM cotton field trials with a broader range ...

2011-01-01

69

ALK, the Key Gene for Gelatinization Temperature, is a Modifier Gene for Gel Consistency in Rice

British Library Electronic Table of Contents (United Kingdom)

Abstract Gelatinization temperature (GT) is an important parameter in evaluating the cooking and eating quality of rice. Indeed, the phenotype, biochemistry and inheritance of GT have been widely studied in recent times. Previous map-based cloning revealed that GT was controlled by ALK gene, which encodes a putative soluble starch synthase II-3. Complementation vector and RNAi vector were constructed and transformed into Nipponbare mediated by Agrobacterium. Phenotypic and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for GT. Meanwhile, amylose content, gel consistency and pasting properties were also affected in transgenic lines. Two of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for GT. ...

2011-01-01

70

Gene expression profiles deciphering rice phenotypic variation between Nipponbare (Japonica) and 93-11 (Indica) during oxidative stress.

An Arabidopsis thaliana methyltransferase Capable of Methylating Farnesoic Acid

Rice is a very important food staple that feeds more than half the world's population. Two major Asian cultivated rice (Oryza sativa L.) subspecies, japonica and indica, show significant phenotypic variation in their stress responses. However, the molecular mechanisms underlying this phenotypic variation are still largely unknown. A common link among different stresses is that they produce an oxidative burst and result in an increase of reactive oxygen species (ROS). In this study, methyl viologen (MV) as a ROS agent was applied to investigate the rice oxidative stress response. We observed that 93-11 (indica) seedlings exhibited leaf senescence with severe lesions under MV treatment compared to Nipponbare (japonica). Whole-genome microarray experiments were conducted, and 1,062 probe sets were identified with gene expression level polymorphisms between the two rice cultivars in addition to differential expression under MV treatment, which were ...

2010-01-08

71

Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori

We previously reported the identification of a new family of plant methyltransferases (MTs), named the SABATH family, that use S-adenosyl-l-methionine (SAM) to methylate a carboxyl moiety or a nitrogen-containing functional group on a diverse array of plant compounds. The Arabidopsis genome alone contains 24 distinct SABATH genes. To identify the catalytic specificities of members of this protein family in Arabidopsis, we screened recombinantly expressed and purified enzymes with a large number of potential substrates. Here, we report that the Arabidopsis thaliana gene At3g44860 encodes a protein with high catalytic specificity towards farnesoic acid (FA). Under steady-state conditions, this farnesoic acid carboxyl methyltransferase (FAMT) exhibits K{sub M} values of 41 and 71 {mu}M for FA and SAM, respectively. A three-dimensional model of FAMT constructed based upon similarity to the experimentally ...

2006-01-01

72

British Library Electronic Table of Contents (United Kingdom)

Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gus...

2011-01-01

73

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

Transfer of Plasmid-Mediated CTX-M-9 from Salmonella enterica Serotype Virchow to Enterobacteriaceae in Human Flora-Associated Rats Treated with Cefixime?

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the ...

1988-10-01

74

An ALMT1 gene cluster controlling aluminium (aluminum) tolerance at the Alt4 locus of rye (Secale cereale L.)

Food animals are a potential source of CTX-M resistance genes for humans. We evaluated the transfer of the bla_CTX-M-9 gene from an animal strain of Salmonella enterica...Full Text Available

2010-01-01

75

The mouse formin (Fmn) gene: Genomic structure, novel exons, and genetic mapping

Aluminium toxicity is a major problem in agriculture worldwide. Among the cultivated triticeae, rye (Secale cereale L.) is one of the most Al-tolerant and represents an important potential source of Al-tolerance for improvement of wheat. The Alt4 Al-tolerance locus of rye contains a cluster of genes...

76

Therapeutic potential of RNA interference in pain medicine

Mutations in the mouse formin (Fmn) gene, formerly known as the limb deformity (ld) gene, give rise to recessively inherited limb deformities and renal malformations or aplasia. The Fmn gene encodes many differentially processed transcripts that are expressed in both adult and embryonic tissues. To study the genomic organization of the Fmn locus, we have used Fmn probes to isolate and characterize genomic clones spanning 500 kb. Our analysis of these clones shows that the Fmn gene is composed of at least 24 exons and spans 400 kb. We have identified two novel exons that are expressed in the developing embryonic limb bud as well as adult tissues such as brain and kidney. We have also used a microsatellite polymorphism from within the Fmn gene to map it genetically to a 2.2-cM interval between D2Mit58 and D2Mit103. 36 refs., 6 figs., 1 tab.

1997-02-01

77

The Zinc Finger SET Domain Gene Prdm14 Is Overexpressed in Lymphoblastic Lymphomas with Retroviral Insertions at Evi32

In recent years RNA interference (RNAi) has rapidly become the most widely used tool for gene knockdown due to its high specificity and potency. RNAi is an evolutionarily conserved mechanism...Full Text Available

2009-01-01

78

The Potential for pathogenicity was present in the ancestor of the Ascomycete subphylum Pezizomycotina

BackgroundAKXD recombinant inbred strains of mice have proven to be very useful in the identification of potential oncogenes and tumor suppressors involved in the development of...Full Text Available

79

Silica-Based Nanoparticle Uptake and Cellular Response by Primary Microglia

BackgroundPrevious studies in Ascomycetes have shown that the function of gene families of which the size is considerably larger in extant pathogens than in non-pathogens could be...Full Text Available

80

Sexual differentiation of the zebra finch song system: potential roles for sex chromosome genes

BackgroundSilica nanoparticles (SiNPs) are being formulated for cellular imaging and for nonviral gene delivery in the central nervous system (CNS), but it is unclear what potential...Full Text Available

2010-05-01

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81

Prognostic gene network modules in breast cancer hold promise

BackgroundRecent evidence suggests that some sex differences in brain and behavior might result from direct genetic effects, and not solely the result of the organizational effects...Full Text Available

82

Multidrug resistant Kluyvera ascorbata septicemia in an adult patient: a case report

A substantial proportion of lymph node-negative patients who receive adjuvant chemotherapy do not derive any benefit from this aggressive and potentially toxic treatment. However, standard histopathological...Full Text Available

2010-01-01

83

Localizing potentially active post-transcriptional regulations in the Ewing's sarcoma gene regulatory network

IntroductionKluyvera ascorbata has become increasingly significant due to its potential to cause a wide range of infections, as well as its ability to transfer gene...Full Text Available

84

Introduction of the CIITA gene into tumor cells produces exosomes with enhanced anti-tumor effects

BackgroundA wide range of techniques is now available for analyzing regulatory networks. Nonetheless, most of these techniques fail to interpret large-scale transcriptional data...Full Text Available

85

Detecting variants with Metabolic Design, a new software tool to design probes for explorative functional DNA microarray development

Exosomes are small membrane vesicles secreted from various types of cells. Tumor-derived exosomes contain MHC class I molecules and tumor-specific antigens, receiving attention as a potential cancer...Full Text Available

2011-05-31

86

An ALMT1 Gene Cluster Controlling Aluminum Tolerance at the Alt4 Locus of Rye (Secale cereale L.)

BackgroundMicroorganisms display vast diversity, and each one has its own set of genes, cell components and metabolic reactions. To assess their huge unexploited metabolic potential...Full Text Available

87

Effect of an Ostertagia ostertagi infection on the transcriptional stability of housekeeping genes in the bovine abomasum

Aluminum toxicity is a major problem in agriculture worldwide. Among the cultivated Triticeae, rye (Secale cereale L.) is one of the most Al tolerant and represents an important potential...Full Text Available

2008-05-01

88

British Library Electronic Table of Contents (United Kingdom)

Quantitative Real-Time PCR (qRT-PCR) is a widely used tool to study host responses against parasites. A crucial step in the gene quantification process is the normalization of the expression data against stable housekeeping genes (HKGs). However, in recent years, several reports have showed that the transcriptional levels of such HKGs can change dramatically, especially when cellular changes appear in the tissues investigated. The aim of the current study was to assess the variability of 11 putative HKGs in bovine abomasal tissue during an infection with the parasitic nematode Ostertagia ostertagi. Gene transcription levels of selected potential HKGs were measured by qRT-PCR and the expression stabilities evaluated using geNorm, NormFinder, and The Mann-Whitney-U test. The analysis showed ...

2011-01-01

89

Potentially Inappropriate Prescribing of Benzodiazepines for Older Adults and Risk of Falls During a Hospital Stay: A Descriptive Study

Mapping cis-Regulatory Domains in the Human Genome UsingMulti-Species Conservation of Synteny

Background:Falls have been identified as a potential adverse event associated with the administration of psychotropic medications to older patients.Objective:The...Full Text Available

2009-07-01

90

Human tissue factor pathway inhibitor (TFPI) gene: Complete genomic structure and localization on the genetic map of chromosome 2q

Our inability to associate distant regulatory elements with the genes that they regulate has largely precluded their examination for sequence alterations contributing to human disease. One major obstacle is the large genomic space surrounding targeted genes in which such elements could potentially reside. In order to delineate gene regulatory boundaries we used whole-genome human-mouse-chicken (HMC) and human-mouse-frog (HMF) multiple alignments to compile conserved blocks of synteny (CBS), under the hypothesis that these blocks have been kept intact throughout evolution at least in part by the requirement of regulatory elements to stay linked to the genes that they regulate. A total of 2,116 and 1,942 CBS>200 kb were assembled for HMC and HMF respectively, encompassing 1.53 and 0.86 Gb of human sequence. To support the existence of complex long-range regulatory domains ...

2005-06-13

91

Molecular analysis of polymerase gamma gene and mitochondrial polymorphism in fertile and subfertile men

Tissue factor pathway inhibitor (TFPI), a protease inhibitor that circulates in association with plasma lipoproteins (VLDL, LDL and HDL), helps to regulate the extrinsic blood coagulation cascade. The authors have cloned a 125-kb genomic region containing the entire human TFPI gene on six overlapping cosmids and prepared a restriction map of this contig to clarify gene structure. More than half (45 kb) of the 85-kb gene is occupied with 5[prime] noncoding elements: coding begins at exon 3. A HindIII RFLP identified with one cosmid was genotyped in the CEPH panel of 559 reference families. Linkage analysis using markers on human chromosome 2 located the TFPI gene on 2q, 36 cM proximal to D2S43(pYNZ15) and 13 cM distal to the crystalline [gamma]-polypeptide locus CRYGP1(p5G1). 31 refs., 3 figs., 3 tabs.

1993-08-01

92

British Library Electronic Table of Contents (United Kingdom)

Summary CAG trinucleotide repeat length in the nuclear polymerase gamma gene (POLg) has been shown to be associated with men with reduced fertility. The present study investigated the frequency of CAG repeat length genotypes and three exonuclease motifs of the POLg in relation to the frequency of mitochondrial nucleotide substitutions. DNA from semen samples of 93 normozoospermic men and 192 non-normozoospermic men was isolated and the specific regions of the genes were amplified by polymerase chain reactions (PCR) and sequenced to identify mutations. The genotypic frequencies of pooled POLg CAG repeat lengths, =10/!=10 heterozygotes and !=10/!=10 homozygotes, were significantly different between normozoospermic and non-normozoospermic men (p p POLg genotype. Of the 17 men with non-synonym...

2006-01-01

93

Identification and characterization of retinoid-active short-chain dehydrogenases/reductases in Drosophila melanogaster

British Library Electronic Table of Contents (United Kingdom)

Background In chordates, retinoid metabolism is an important target of short-chain dehydrogenases/reductases (SDRs). It is not known whether SDRs play a role in retinoid metabolism of protostomes, such as Drosophila melanogaster. Methods Drosophila genome was searched for genes encoding proteins with ?50% identity to human retinol dehydrogenase 12 (RDH12). The corresponding proteins were expressed in Sf9 cells and biochemically characterized. Their phylogenetic relationships were analyzed using PHYLIP software. Results A total of six Drosophila SDR genes were identified. Five of these genes are clustered on chromosome 2 and one is located on chromosome X. The deduced proteins are 300 to 406 amino acids long and are associated with microsomal membranes. They recognize all-trans-retinaldehyd...

2009-01-01

94

Cloning and characteristics of Brn1 gene in Curvularia lunata causing leaf spot in maize

British Library Electronic Table of Contents (United Kingdom)

The full length cDNA of the Brn1 was first cloned, and then expression of the Brn1 was analyzed and the function was identified by silencing technology. Results show that the full length cDNA of the C. lunata Brn1 gene contains 1001 base pairs and an 801?bp open reading frame encoding 267 amino acids. Semi-quantitative PCR analysis shows that the expression of Brn1 at 96?h is significantly higher than at 24 and 72?h (p?Brn1-silenced transformants were light brown in culture filtrate, and have significantly reduced toxin production relative to the wild-type. These results imply that Brn1 gene in C. lunata is not only involved in 1,8-dihydroxynaphthalene melanin synthesis, but is also relatively associated with toxin biosynthesis of the pathogen.

2011-01-01

95

An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes

Absence of the A4 peptide in the G4 glycinin subunit of soybean cultivar Enrei is caused by a point mutation in the Gy4 gene

The authors have identified a protein present only in erythroid cells that binds to two adjacent sites within an enhancer region of the chicken {beta}-globin locus. Mutation of the sites, so that binding by the factor can no longer be detected in vitro, leads to a loss of enhancing ability, assayed by transient expression in primary erythrocytes. Binding sites for the erythroid-specific factor (Eryf1) are found within regulatory regions for all chicken globin genes. A strong Eryf1 binding site is also present within the enhancer of at least one human globin gene, and proteins from human erythroid cells (but not HeLa cells) bind to both the chicken and the human sites.

1988-08-01

96

Scientific Electronic Library Online (English)

Abstract in english Functional properties of soy proteins for food are closely related to the composition of their storage protein subunits. Using base excision sequence scanning (BESS), we show that the absence of the A4 peptide in the G4 glycinin subunit of the soybean (Glycine max L.) cultivar Enrei was caused by the same point mutation in the Gy4 gene as previously reported in the soybean cultivar Raiden. Although the genetic relationship between Raiden and Enrei is not known, the same p (more) oint mutation in their Gy4 genes may indicate that they probably share a related origin. The application of BESS to identify single nucleotide polymorphisms (SNPs) as co-dominant markers for marker-assisted selection (MAS) of a recessive null allele is also discussed.

2005-09-01

97

A signature of six genes highlights defects on cell growth and specific metabolic pathways in murine and human hepatocellular carcinoma

British Library Electronic Table of Contents (United Kingdom)

Hepatocellular carcinoma (HCC) represents a major health problem as it afflicts an increasing number of patients worldwide. Albeit most of the risk factors for HCC are known, this is a deadly syndrome with a life expectancy at the time of diagnosis of less than 1?year. Definition of the molecular principles governing the neoplastic transformation of the liver is an urgent need to facilitate the clinical management of patients, based on innovative methods to detect the disease in its early stages and on more efficient therapies. In the present study, we have combined the analysis of a murine model and human samples of HCC to identify genes differentially expressed early in the process of hepatocarcinogenesis, using a microarray-based approach. Expression of 190 genes was impaired in murine ...

2011-01-01

98

The Differentially Expressed Genes by Radiotherapy in the Patients with Uterine Cervix Cancer

A PAC containing the human mitochondrial DNA polymerase gamma gene (POLG) maps to chromosome 15q25

Purpose : To detect differentially expressed genes in the patients with uterine cervical cancer during the radiation therapy. Materials and Methods : In patients with biopsy proven uterine cervical cancer, we took a tumor tissue just before radiation therapy and at 40 minutes after external irradiation of 1.8 Gy. Total RNAs isolated from non-irradiated and irradiated tumor tissue samples were analyzed using the differential-display reverse transcription-polymerase chain reaction (DDRT-PCR). Complementary DNA (cDNA) fragments corresponding to differentially expressed messenger RNAs(mRNAs) were eluted, and cloned. The differential expression of the corresponding mRNAs was confirmed by reverse northern blot. Differentially expressed cDNA bands were sequenced. Nucleotide sequence data were analyzed in the Gene Bank and EMBL databases via the BLAST network server to identify homologies to known genes or cDNA ...

2001-12-15

99

Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.).

The human mitochondrial DNA (mtDNA) is a closed circular, 16,569-bp double-stranded DNA, encoding 13 genes whose protein products are subunits of the oxidative phosphorylation system required for synthesis of most of the ATP consumed by eukaryotic cells. Point mutations of the mtDNA that cause multi-tissue, loss-of-energy syndromes, called mitochondrial encephalomyopathies (e.g., MERRF and MELAS), have been identified. In addition, large-scale deletions of the human mtDNA have been identified and are the molecular bases for the neonatal and adolescent onset loss-of-energy syndromes Pearson and Kearns-Sayer, respectively. 5 refs., 1 fig.

1997-03-01

100

Identification of single nucleotide polymorphisms in candidate genes for growth and reproduction in a nonmodel organism; the Atlantic cod, Gadus morhua

High salinity is one of the main factors limiting cotton growth and productivity. The genes that regulate salt stress in TM-1 upland cotton were monitored using microarray and real-time PCR (RT-PCR) with samples taken from roots. Microarray analysis showed that 1503 probe sets were up-regulated and 1490 probe sets were down-regulated in plants exposed for 3h to 100mM NaCl, and RT-PCR analysis validated 42 relevant/related genes. The distribution of enriched gene ontology terms showed such important processes as the response to water stress and pathways of hormone metabolism and signal transduction were induced by the NaCl treatment. Some key regulatory gene families involved in abiotic and biotic sources of stress such as WRKY, ERF, and JAZ were differentially expressed. Our transcriptome analysis might provide some useful insights into salt-mediated signal transduction pathways in cotton and offer a ...

2011-04-30

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101

Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

Recent technological developments have facilitated intensified searches for genetic markers under selection in nonmodel species. Here, we present an approach for the identification of candidate gene variation in nonmodel organisms. We report on the characterization of 82 single nucleotide polymorphisms (SNPs) and on the development of a specific genotyping assay for 30 SNPs in 18 candidate genes for growth and reproduction in Atlantic cod (Gadus morhua). These markers can be used for scanning natural populations for signatures of selection in both contemporary and archived historical samples, for example in retrospective studies assessing the effects of environmental changes, such as increasing temperatures, and selection imposed by high fishing pressure. Furthermore, these gene markers may be of interest to aquaculture, serving as a starting point for linking phenotypic traits important for productivity with genotypes and ...

2011-01-01

102

Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma

BackgroundBiodiesel or ethanol derived from lipids or starch produced by microalgae may overcome many of the sustainability challenges previously ascribed to petroleum-based fuels and first generation plant-based biofuels. The paucity of microalgae genome sequences, however, limits gene-based biofuel feedstock optimization studies. Here we describe the sequencing and de novo transcriptome assembly for the non-model microalgae species, Dunaliella tertiolecta, and identify pathways and genes of importance related to biofuel production.ResultsNext generation DNA pyrosequencing technology applied to D. tertiolecta transcripts produced 1,363,336 high quality reads with an average length of 400 bases. Following quality and size trimming, ~ 45% of the high quality reads were assembled into 33,307 isotigs with a 31-fold coverage and 376,482 singletons. Assembled sequences and singletons were subjected to BLAST similarity searches ...

2011-03-14

103

A variable gene delivery carrier-biotinylated chitosan/polyethyleneimine

Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of ...

1989-01-01

104

The ZNF804A gene: characterization of a novel neural risk mechanism for the major psychoses.

A variable gene delivery system has been developed based on conjugating chitosan to biotin through a functionalized poly(ethylene glycol) (PEG) spacer, which can be used to further bind different molecules on the outer layer of a polymer/DNA complex by streptavidin (SA)-biotin linkage. In this study, TAT-conjugated SA was used as the model molecule to prove the conjugation function of the prepared complex. In addition, low-molecular-weight poly(ethyleneimine) (PEI) was added into the polymer/DNA complex to increase the transfection efficiency. The results of the luciferase assay show that the transfection efficiency of the prepared complex was significantly correlated with the amount of PEI and was further enhanced when TAT was conjugated to the complex by SA-biotin linkage. Considered to have negligible cytotoxic effects, the variable gene delivery complex prepared in this study would be of considerable potential as ...

2010-12-01

105

The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth

Schizophrenia and bipolar disorder share genetic risk, brain vulnerability, and clinical symptoms. The ZNF804A risk variant, rs1344706, confers susceptibility for both disorders. This study aimed to identify neural mechanisms common to both schizophrenia and bipolar disorder through this variant's potential effects on cortical thickness, white matter tract integrity, and cognitive function. Imaging, genetics, and cognitive measures were ascertained in 62 healthy adults aged between 18 and 59 years. High-resolution multimodal MRI/DTI imaging was used to measure cortical thickness and major frontotemporal and interhemispheric white matter tracts. The general linear model was used to examine the influence of the ZNF804A rs1344706 risk variant on cortical thickness, white matter tract integrity, and cognitive measures. Individuals homozygous for the risk variant ('A' allele) demonstrated reduced cortical gray matter thickness in the superior ...

2011-04-27

106

Environmental Research Database

The Myc oncoprotein is a potent inducer of cell growth, cell cycle progression, and apoptosis. While many direct Myc target genes have been identified, the molecular determinants of Myc’s transcriptional...Full Text Available

2007-03-01

107

Plant Sulphur Nutrition

DescriptionThe recent sequencing of the complete genomes of several streptomycete species revealed the presence of a large number of cryptic' secondary metabolic gene clusters, and led to the realisation that these organisms have the ability to produce many more natural products than had previously been recognised. One of the aims of our work is to identify the physiological signals and regulatory mechanisms responsible for the activation of these 'cryptic' pathways, thus unleashing the full biosynthetic p [continued...

2010-01-31

108

Homozygosity Mapping Reveals Null Mutations in FAM161A as a Cause of Autosomal-Recessive Retinitis Pigmentosa

Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA.

Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal degenerations caused by mutations in at least 45 genes. Using homozygosity mapping, we identified a ∼4 Mb homozygous region...Full Text Available

2010-09-10

109

Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels

A two-step polymerase chain reaction (PCR) procedure with two nested pairs of primers specific for the yadA gene of Yersinia enterocolitica was developed. The PCR assay identified all common pathogenic...Full Text Available

1993-09-01

110

Comparative transcriptional pathway bioinformatic analysis of dietary restriction, Sir2, p53 and resveratrol life span extension in Drosophila

Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived...Full Text Available

2009-01-01

111

Characterization of a Novel Small Molecule Subtype Specific Estrogen-Related Receptor ? Antagonist in MCF-7 Breast Cancer Cells

A multiple comparison approach using whole genome transcriptional arrays was used to identify genes and pathways involved in calorie restriction/dietary restriction (DR) life span extension in Drosophila....Full Text Available

2011-03-15

112

Activation of Metallothioneins and ?-Crystallin/sHSPs in Human Lens Epithelial Cells by Specific Metals and the Metal Content of Aging Clear Human Lenses

BackgroundThe orphan nuclear receptor estrogen-related receptor α (ERRα) is a member of the nuclear receptor superfamily. It was identified through a search for genes...Full Text Available

113

A genome-wide association study of cleft lip with and without cleft palate identifies risk variants near MAFB and ABCA4

PurposeTo identify those metallothionein and α-crystallin/small heat-shock genes induced by toxic metals in human lens cells and to evaluate...Full Text Available

2003-02-01

114

Expression of bacterial luciferase in eukaryotic cells

Case-parent trios were used in a genome wide association study of cleft lip with/without cleft palate (CL/P). SNPs near two genes not previously associated with CL/P [MAFB:...Full Text Available

2010-06-01

115

Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton

Expression of Bacterial luciferase enzyme (lux) in mammalian cells would be a powerful bioreporter protein system for in vivo imaging because eukaryotic luciferases need expensive substrates. However, only a few efforts have been made to express bacterial luciferase enzyme in mammalian cells. As the result of this, we attempted to construct bicistronic vector including two bacterial luciferase genes (LuxA and LuxB) for assessing the potential to be visualized in vitro or in vivo by optical imaging system after transfection to mammalian cells. We designed and synthesized luxA and luxB genes from Photorhabdus Luminescens. To co-express both luxA and luxB genes from a single promoter, we cloned as a bicistronic transcript fused with an internal ribosomal entry site (IRES). This bicistronic transcript was transfected by Superfect to HEK 293T cell line. We also transfected lux A and lux B vector to HEK 293T ...

2005-11-18

116

British Library Electronic Table of Contents (United Kingdom)

Cytoplasmic male sterility (CMS) is a maternally inherited trait that fails to produce functional pollen grains. The CMS system is widely employed to facilitate the utilization of heterosis in major crops. However, little is known about the CMS associated genes in Upland cotton (Gossypium hirsutum). The objective of this study was to compare CMS cotton (CMS-D2) with the cytoplasm from G. harknessii and its isogenic maintainer line with the normal fertile Upland cotton cytoplasm to identify CMS-D2 specific gene(s) and to develop CMS-specific sequence characterized amplified region (SCAR) markers. Based on Southern blot analysis using 10 mitochondrial gene-specific probes (cob, cox2, atp6, atp9, nad3, cox3, atpA, cox1, nad6 and nad9), three probes (cox3, atpA, and nad6) revealed restriction ...

2011-01-01

117

Mapping of the gene encoding the melanocortin-1 ([alpha]-melanocyte stimulating hormone) receptor (MC1R) to human chromosome 16q24. 3 by fluorescence in situ hybridization

Gene linked to Lou Gehrig's disease

[alpha]-Melanocyte stimulating hormone ([alpha]-MSH), a hormone originally named for its ability to regulate pigmentation of melanocytes, is a 13-amino-acid post-translational product of the pro-opiomelanocortin (POMC) gene. [alpha]-MSH and the other products of POMC processing, which share the core heptapeptide amino acid sequence Met-Glu (Gly)-His-Phe-Arg-Trp-Gly (Asp), the adrenocorticotropic hormone (ACTH), [beta]-MSH, and [gamma]-MSH, are collectively referred to as melanocortins. While best known for their effects on the melanocyte (pigmentation) and adrenal cortical cells (steroidogenesis), melanocortins have been postulated to function in diverse activities, including enhancement of learning and memory, control of the cardiovascular system, analgesia, thermoregulation, immunomodulation, parturition, and neurotrophism. To identify the chromosomal band encoding the human melanocortin-1 receptor gene, 1 [mu]g of an ...

1994-01-15

118

Gene expression analysis after low dose ionising radiation exposure of the developing organism

Scientists have just taken a big step toward understanding the cause of Lou Gehrig's disease, one of the most devastating nerve degenerative diseases. A large team of researchers, led by Robert Brown Jr. of Harvards's Massachusetts General Hospital and Robert Horvitz, a Howard Hughes Medical Institute investigator at the Massachusetts Institute of Technology, report in the 4 March Nature that they've identified the gene that causes a hereditary form of the condition, which also goes by the name amyothophic lateral sclerosis (ALS). While most ALS cases - approximately 90% - are apparently sporadic' and not caused by an inherited gene defect, all the patients have such similar symptons that researchers are hopeful that what they learn about hereditary ALS will also apply to the sporadic form, possibly leading to new therapeutic strategies that will help both. It's a very important ...

1993-03-05

119

Chromosomal localization of the human retinoid X receptors

Measuring gene expression using microarrays is relevant to many areas of biology and medicine, such as follow up of developmental stages and diseases onset, and treatment study. Since there can be tens of thousands of distinct probes on an array, each micro array experiment can accomplish the equivalent number of genetic tests in parallel. Arrays have therefore dramatically accelerated many types of investigations. For example, microarrays can be used to identify stress response genes by comparing gene expression in challenged versus normal cells. In the Molecular and Cellular Biology lab (MCB), the micro array experiments are performed within the Genomic Platform, fully equipped to analyse either the behaviour of bacteria during long space flight, the effect of low dose ionising radiation on the developing organism in mice, or the human individual radiation sensitivity. For the low dose effect, two ...

2007-09-01

120

DNA barcoding as a tool for species identification in three forensic wildlife cases in South Africa

The recently described retinoid X receptors (RXRs) respond to the novel retinoid 9-cis-retinoic acid and also serve as heterodimeric partners for the vitamin D, thyroid hormone, and retinoic acid receptors (VDR, TR, and RAR, respectively). In this work, the authors report high-resolution localization of the human RXR genes within cytogenetic bands and also within a standard reference map of cosmid DNA markers on human chromosomes. They have determined the location of the human RXR genes by pairwise hybridization of the RXR cosmids and reference markers, using fluorescence in situ hybridization. They localized (i) RXR[alpha] (RXRA) to chromosome 9 band q34.3; (ii) RXR[beta] (RXRB) to chromosome 6 band 21.3; and (iii) RXR[gamma] (RXRG) to chromosome 1 band q22-q23. Six retinoid-responsive transcription factors have been identified so far, including three retinoic acid receptors in addition to the three RXRs. Interestingly, ...

1994-04-01

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121

British Library Electronic Table of Contents (United Kingdom)

Poaching of wildlife animals for subsistence and commercial purposes has lead to population declines in Africa. In forensic cases, a need exists to identify the species of origin of carcasses, meat or blood. In the study presented here, the mitochondrial COI gene was sequenced to determine the species of unknown samples in three suspect South African forensic wildlife cases. In two cases the unknown samples were identified as originating from domestic cattle (Bos taurus) and in the third case the sample was identified as common reedbuck (Redunca arundinum). This is the first report of the COI sequence of common reedbuck. The study highlights the need for accurate wildlife reference material from each country in order to convict wildlife cases.

2011-01-01

122

Gene Repressive Activity of RIP140 Through Direct Interaction with CDK8.

The expression pattern of scygonadin during the ontogenesis of Scylla paramamosain predicting its potential role in reproductive immunity

Receptor interacting protein 140 (RIP140) is a coregulator for numerous nuclear receptors and transcription factors and primarily exerts gene-repressive activities on various target genes. We previously identified a spectrum of posttranslational modifications on RIP140 that augment its property and biological activity. In T(3)-triggered biphasic regulation of cellular retinoic acid binding protein 1 (Crabp1) gene along the course of fibroblast-adipocyte differentiation, we found TRAP220(MED1) critical for T(3)-activated chromatin remodeling whereas RIP140 essential for T(3)-repressive chromatin remodeling of this gene promoter. In this current study, we aim to examine whether and how RIP140 replaces TRAP220(MED1) on the CrabpI promoter in differentiating adipocyte cultures. We find increasing recruitment of RIP140 to this promoter, with corresponding reduction in TRAP220(MED1) ...

2011-08-25

123

British Library Electronic Table of Contents (United Kingdom)

The antimicrobial peptide scygonadin (Scy) was first isolated from the gonad of Scylla serrata and its gene is predominantly expressed in the ejaculatory duct of adult males. Thus, its function was predicted to be associated with reproductive immunity, but this is still unclear and needs further investigation. In our study, the expression pattern of Scy at different developmental stages of both male and female S. paramamosain was investigated, so that the potential function of this peptide could be examined. Using real-time quantitative PCR, Scy mRNA transcripts were demonstrated obviously in the vulnerable embryos and larvae-zoea I but very weakly detected in the larvae-zoea III, megalops and juveniles. The gene expression pattern showed a decreasing trend during the early developmental s...

2011-01-01

124

Safety review of conceptual fusion power plants

Safety review of conceptual fusion power plants

The potential public safety impacts from accidents in conceptual fusion power plants were investigated. Fusion was found to have some potential for accidents, as does any energy generating system. Functions of fusion power plants were identified that possess sufficient potential for an accidental release of toxic materials to the environment. An assessment was made of the impact of the potential accidents and recommendations are included for R and D that will allow incorporation of safety concerns in fusion power plant design. This work was based on a review of information available in conceptual design documents of fusion reactor systems.

1976-11-01

125

Antimicrobial potential of Glycyrrhiza glabra roots

The potential public safety impacts from accidents in conceptual fusion power plants were investigated. Fusion was found to have some potential for accidents, as does any energy generating system. Functions of fusion power plants were identified that possess sufficient potential for an accidental release of toxic materials to the environment. An assessment was made of the impact of the potential accidents and recommendations are included for R and D that will allow incorporation of safety concerns in fusion power plant design. This work was based on a review of information available in conceptual design documents of fusion reactor systems.

126

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The present study was aimed to investigate antimicrobial potential of Glycyrrhiza glabra roots. Antimycobacterial activity of Glycyrrhiza glabra was found at 500mg/mL concentration. Bioactivity guided phytochemical analysis identified glabridin as potentially active against both Mycobacterium tuberculosis H37Ra and H37Rv strains at 29.16mg/mL concentration. It exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. Our results indicate potential use of licorice as antitubercular agent through systemic experiments and sophisticated anti-TB assay.

2008-01-01

127

Laboratory Evaluation of Base Materials for Neutralization of the Contaminated Aquifer at the F-Area Seepage Basins

Coal to liquids technology (Overview - Research)

Laboratory studies were performed to support field-testing of base injection into the F-Area Seepage Basins groundwater. The general purpose of these experiments is to provide information to guide the test of base injection and to identify potential adverse effects.

2001-09-11

128

Wastenet

... After completing a review into direct coal liquefaction research carried out in Australia and internationally, CSIRO has identified potential new science and technology developments that may improve the cost and viability of CTL in a greenhouse gas-constrained environment. We are conducting research to: determine the optimum ...

129

Biobank resources for future patient care: developments, principles and concepts

Bacterial Competition for Human Nasal Cavity Colonization: Role of Staphylococcal agr Alleles

The aim of the overview is to give a perspective of global biobank development is given in a view of positioning biobanking as a key resource for healthcare to identify new potential markers that can...Full Text Available

130

A Novel Mouse Model of Red Blood Cell Storage and Post-Transfusion in Vivo Survival

We examined the bacterial aerobic nasal flora of 216 healthy volunteers to identify potential competitive interactions among different species, with special emphasis on the influence of staphylococcal...Full Text Available

2003-01-01

131

Technical area status report for chemical/physical treatment. Volume 2, Appendices

BackgroundStorage of RBCs is necessary for an adequate blood supply. However, reports have identified potential negative sequelae of transfusing stored RBCs. An animal...Full Text Available

2009-08-01

132

Oxidation of Elemental Sulfur by Fusarium solani Strain THIF01 Harboring Endobacterium Bradyrhizobium sp.

These Appendices describe various technologies that may be applicable to the Mixed Waste Treatment Plant (MWTP) Chemical/Physical Treatment System (CPTS). These technologies were identified by the CPTS Technical Support Group (TSG) as potentially applicable to a variety of separation, volume reduction, and decontamination requirements. The purpose was to identify all available and developing technologies, and their characteristics, for subsequent evaluation for specific requirements identified for the CPTS. However, the technologies described herein are not necessarily all inclusive, nor are they necessarily all applicable.

1993-08-01

133

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Nineteen fungal strains having an ability to oxidize elemental sulfur in mineral salts medium were isolated from deteriorated sandstones of Angkor monuments. These fungi formed clearing zone on agar medium supplemented with powder sulfur due to the dissolution of sulfur. Representative of the isolates, strain THIF01, was identified as Fusarium solani on the basis of morphological characteristics and phylogenetic analyses. PCR amplification targeting 16S rRNA gene and analyses of full 16S rRNA gene sequence indicated strain THIF01 harbors an endobacterium Bradyrhizobium sp.; however, involvement of the bacterium in the sulfur oxidation is still unclear. Strain THIF01 oxidized elemental sulfur to thiosulfate and then sulfate. Germination of the spores of strain THIF01 was observed in a liqui...

2010-01-01

134

Isolation and characterization of an ornithine aminotransferase-related sequence (OATL3) mapping to 10q26

Human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R): Localization to chromosome band 7p14 and integration into the cytogenetic, physical, and genetic map of chromosome 7

The authors used a near full-length human ornithine [delta]-aminotransferase cDNA, huOAT6, as a probe under low stringency hybridization conditions to identify a new autosomal ornithine [delta]-aminotransferase-related sequence (OATL3). Cloning and characterization of this sequence reveal it to be a partial nonprocessed pseudogene corresponding to exon 3 and flanking intronic sequences of the ornithine [delta]-aminotransferase structural gene. Using somatic cell hybrids and fluorescence in situ hybridization, they mapped OATL3 to 10q26, adjacent to the ornithine [delta]-aminotransferase structural gene locus. 13 refs., 3 figs.

1993-08-01

135

Detection of the first gross CDC73 germline deletion in an HPT-JT syndrome family

The gene encoding the human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R1) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human/rodent somatic cell hybrid mapping panel. This assignment was confirmed and the gene localized to chromosome band 7p14 by fluorescence in situ hybridization. A yeast artificial chromosome containing ADCYAP1R1 was identified in the CEPH {open_quotes}B{close_quotes} Mega-YAC library. This YAC includes two highly polymorphic dinucleotide repeat sequences that will facilitate genetic studies of the contribution of ADCYAP1R1 in disease states of the central nervous and neuroendocrine systems. 13 refs., 1 fig.

1994-10-01

136

British Library Electronic Table of Contents (United Kingdom)

Abstract Hereditary primary hyperparathyroidism (HPT) may develop as a solitary endocrinopathy (FIHP) or as part of multiple endocrine neoplasia Type 1, multiple endocrine neoplasia Type 2A, or hereditary HPT-jaw tumor syndrome. Inactivating germline mutations of the tumor suppressor gene CDC73 account for 14 and 50% of all FIHP and HPT-JT patients, respectively, and have also been found in almost 20% of apparently sporadic parathyroid carcinoma patients. Although more than 60 independent germline mutations have been described, to date no rearrangement affecting the CDC73 locus has been identified. By means of multiplex-PCR we found a large germline deletion affecting the whole gene in a two-generation HPT-JT family. Subsequently array-CGH and specific PCR analysis determined that the muta...

2011-01-01

137

Relationship between O6-alkylguanine-DNA alkyltransferase activity and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutation, transformation, and cytotoxicity in C3H/10T1/2 cells expressing exogenous alkyltransferase genes.

Nonsyndromic bilateral and unilateral optic nerve aplasia: first familial occurrence and potential implication of CYP26A1 and CYP26C1 genes

While a great deal of evidence has directly implicated the importance of O6-alkylation of guanine in the mutagenicity of alkylating agents, evidence demonstrating the oncogenic potential of this lesion...Full Text Available

1992-12-01

138

Environmental analysis of endocrine disrupting effects from hydrocarbon contaminants in the ecosystem. 1997 annual progress report

PurposeOptic nerve aplasia (ONA, OMIM 165550) is a very rare unilateral or bilateral condition that leads to blindness in the affected eye, and is usually associated with other ocular...Full Text Available

139

Inventory of Tank Farm equipment stored or abandoned aboveground

'The overall objective of the basic research grant is to characterize the potential of common hydrocarbon contaminants in ecosystems to act as endocrine disruptors. The three major lines of research include (1) a biotechnology based screening system to identify potential hormone mimics and antagonists; (2) an animal screening system to identify biomarkers of endocrine effects. and (3) a literature review to identify compounds at a variety of DOE sites that need to be examined for endocrine disrupting effects. By relating results obtained from this research project to contamination problems at various DOE sites. CBR will provide data and information on endocrine disrupting contaminants to DOE for consideration in risk analyses for determining clean-up levels and priorities needed at the sites.'

1997-01-01

140

Roles for Rat Hepatocyte Malignant Transforming Factor (HMTF) in Late Stage of Hepatocarcinogenesis.

This document provides an inventory of Tank Farm equipment stored or abandoned aboveground and potentially subject to regulation. This inventory was conducted in part to ensure that Westinghouse Hanford Company (WHC) does not violate dangerous waste laws concerning storage of potentially contaminated equipment/debris that has been in contact with dangerous waste. The report identifies areas inventoried and provides photographs of equipment.

1994-10-12

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141

Evaluation of Toll-like receptors 3 (c.1377C/T) and 9 (G2848A) gene polymorphisms in cervical cancer susceptibility

In a previous study, to identify genes of importance for hepatocellular carcinogenesis, and especially for processes involved in malignant transformation, the authors investigated differences in gene expression between adenomas and carcinomas by DNA microarray. In the present study, the authors investigated AW434047, one of the sequences that was upregulated in carcinomas. The investigation led to the identification of a novel gene, which the authors named hepatocyte malignant transforming factor (HMTF), of unknown function whose expression was increased in hepatocellular carcinomas. Northern blot and in situ hybridization also demonstrated high levels of HMTF in rat hepatocellular carcinoma (HCC) cell lines, lymphocytes in the spleen, colon mucosal epithelia, spermatocytes, and granule cells of the hippocampus. Reduction of HMTF by RNA interference (RNAi) in N1 cells, an HCC cell line, caused ...

2011-09-20

142

British Library Electronic Table of Contents (United Kingdom)

Cervical cancer is emerging as a leading cause of morbidity and mortality in women worldwide. Toll-like Receptor (TLR) gene polymorphisms may contribute to subsequent inter-individual variability in cancer susceptibility. The present study aimed to identify the role of TLR 3 (c.1377C/T) [rs3775290] and TLR 9 (G2848A) [rs352140] gene polymorphisms in the risk of developing cervical cancer in North India. Peripheral blood samples were collected from 200 histopathologically confirmed cervical cancer patients from North India and 200 unrelated, cancer-free, age-matched healthy female controls of similar ethnicity. Genomic DNA was extracted using the salting-out method, and genotyped for TLR 3 and TLR 9 using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). O...

2011-01-01

143

Sources of toxicity and exposure information for identifying chemicals of high concern to children

Identification of tumor-initiating cells in a p53-null mouse model of breast cancer.

Due to the large number of chemicals in commerce without adequate toxicity characterization data, coupled with an ineffective federal policy for chemical management in the United States, many states are grappling with the challenge to identify toxic chemicals that may pose a risk to human health and the environment. Specific populations (e.g., children, elderly) are particularly sensitive to these toxic chemicals. In 2008, the Children's Safe Product Act (CSPA) was passed in Washington State. The CSPA included specific requirements to identify High Priority Chemicals (HPCs) and Chemicals of High Concern to Children (CHCCs). To implement this legislation, a methodology was developed to identify HPCs from authoritative scientific and regulatory sources on the basis of toxicity criteria. Another set of chemicals of concern was then identified from authoritative sources, based on their ...

2010-11-01

144

Summary of research on microbiological processes. International Energy Agency Subtask D, final report

Using a syngeneic p53-null mouse mammary gland tumor model that closely mimics human breast cancer, we have identified, by limiting dilution transplantation and in vitro mammosphere assay, a Lin(-)CD29(H)CD24(H) subpopulation of tumor-initiating cells. Upon subsequent transplantation, this subpopulation generated heterogeneous tumors that displayed properties similar to the primary tumor. Analysis of biomarkers suggests the Lin(-)CD29(H)CD24(H) subpopulation may have arisen from a bipotent mammary progenitor. Differentially expressed genes in the Lin(-)CD29(H)CD24(H) mouse mammary gland tumor-initiating cell population include those involved in DNA damage response and repair, as well as genes involved in epigenetic regulation previously shown to be critical for stem cell self-renewal. These studies provide in vitro and in vivo data that support the cancer stem cell (CSC) hypothesis. Furthermore, this p53-null mouse mammary ...

2008-06-15

145

Summary of research on microbiological processes

Storage of thermal energy in aquifers has obvious benefits of saving energy and decreasing the consumption of fossil fuels. However, aquifer thermal energy storage (ATES), which involves groundwater aquifers as the storage medium for heat or chill, impinges on the environment. A literature review of pertinent microbiology publications (Hicks and Stewart, 1988) identified the potential for the interaction of ATES systems and microbiological processes to create a source of infectious diseases and the potential for damage to the environment. In addition, the review identified a potential for microbiological processes to develop conditions that would interfere with the operation of an ATES system. As a result of this research effort, investigators from Finland, Germany, Switzerland, and the United States have examined several ATES systems in operation and have observed that the ATES ...

1992-09-01

146

Interspecies Trait Genetics Reveals Association of Adcy8 with Mouse Avoidance Behavior and a Human Mood Disorder

Storage of thermal energy in aquifers has obvious benefits of saving energy and decreasing the consumption of fossil fuels. However, aquifer thermal energy storage (ATES), which involves groundwater aquifers as the storage medium for heat or chill, impinges on the environment. A literature review of pertinent microbiology publications (Hicks and Stewart, 1988) identified the potential for the interaction of ATES systems and microbiological processes to create a source of infectious diseases and the potential for damage to the environment. In addition, the review identified a potential for microbiological processes to develop conditions that would interfere with the operation of an ATES system. As a result of this research effort, investigators from Finland, Germany, Switzerland, and the United States have examined several ATES systems in operation and have observed that the ATES ...

1992-09-01

147

British Library Electronic Table of Contents (United Kingdom)

Background Identifying susceptibility genes for endophenotypes by studying analogous behaviors across species is an important strategy for understanding the pathophysiology underlying psychiatric disorders. This approach provides novel biological pathways plus validated animal models critical for selective drug development. One such endophenotype is avoidance behavior. Methods In the present study, novel automated registration methods for longitudinal behavioral assessment in home cages are used to screen a panel of recently generated mouse chromosome substitution strains that are very powerful in quantitative trait loci (QTL) detection of complex traits. In this way, we identified chromosomes regulating avoidance behavior (increased sheltering preference) independent of motor activity lev...

2009-01-01

148

Genomic survey of prepulse inhibition in mouse chromosome substitution strains

British Library Electronic Table of Contents (United Kingdom)

Prepulse inhibition (PPI) is a measure of sensorimotor gating, a pre-attentional inhibitory brain mechanism that filters extraneous stimuli. Prepulse inhibition is correlated with measures of cognition and executive functioning, and is considered an endophenotype of schizophrenia and other psychiatric illnesses in which patients show PPI impairments. As a first step toward identifying genes that regulate PPI, we performed a quantitative trait locus (QTL) screen of PPI phenotypes in a panel of mouse chromosome substitution strains (CSSs). We identified five CSSs with altered PPI compared with the host C57BL/6J strain: CSS-4 exhibited decreased PPI, whereas CSS-10, -11, -16 and -Y exhibited higher PPI compared with C57BL/6J. These data indicate that A/J chromosomes 4, 10, 11, 16 and Y harbor...

2009-01-01

149

An assessment of the building sector efficiency resource for the Town of Handlova

Role of Fgf receptor 2c in adipocyte hypertrophy in mesenteric white adipose tissue

Purpose of this assessment for this town in the Slovak Republic is to develop information on the building sector energy efficiency and improvement potential for use by town management to support decisions on providing energy services to Handlova citizens. Objectives were to characterize baseline space and water heat energy use and efficiency improvement potential in the residential and nonresidential building sectors. Intention was to identify major areas of efficiency improvement potential and cost-effectiveness. Four levels of energy and fuel prices were used. A significant efficiency resource exists in the space and water heating end uses in these building sectors; it amounts to 42% of the total building sector space and water heat energy consumption.

1995-10-01

150

British Library Electronic Table of Contents (United Kingdom)

Fgf receptor 2c (Fgfr2c) was expressed in mature adipocytes of mouse white adipose tissue (WAT). To examine the role of Fgfr2c in mature adipocytes, we generated adipocyte-specific Fgfr2 knockout (Fgfr2 CKO) mice. The hypertrophy impairment of adipocytes in the mesenteric WAT but not in the subcutaneous WAT and decreased plasma free fatty acid (FFA) levels were observed in Fgfr2 CKO mice. Although the expression of genes involved in adipocyte differentiation and lipid metabolism in the mesenteric WAT was essentially unchanged, the expression of uncoupling protein 2 potentially involved in energy dissipation was significantly increased. Among potential Fgf ligands for Fgfr2c, Fgf9 was preferentially expressed in the mesenteric WAT. The present findings indicate that Fgfr2c potentially activ...

2008-01-01

151

The expression profile of TLR9 mRNA and CpG ODNs immunostimulatory actions in the teleost gilthead seabream points to a major role of lymphocytes

British Library Electronic Table of Contents (United Kingdom)

Abstract. The potential effects of synthetic unmethylated oligodeoxynucleotides (ODN) containing CpG motifs, mimicking bacterial DNA, has never been evaluated on the immune response in the teleost fish gilthead seabream (Sparus aurata), the most important fish species in Mediterranean aquaculture. First, binding and competition studies have demonstrated that binding is saturated and promiscuous, suggesting the participation of several receptors. Moreover, leucocyte cytotoxic (NCC) activity, production of ROIs (reactive oxygen intermediates), and expression of immune-relevant genes was greatly primed by ODNs. Focusing on the mechanism, the TLR9 gene is widely distributed in seabream tissues and differently regulated in vitro by several stimuli. Moreover, and for the first time in fish, TLR9...

2008-01-01

152

Effects of ozone on gene expression and lipid peroxidation in adults and larvae of the red flour beetle (Tribolium castaneum)

British Library Electronic Table of Contents (United Kingdom)

Ozone has in recent years been increasingly investigated for its potential use in the control of insect pests of stored cereals. Ozone is a powerful oxidizing agent that can react directly, or via production of reactive oxygen species, with proteins, DNA and double bonds of polyunsaturated fatty acids (PUFA). The aim of the present study was to investigate the mode of action in ozone toxicity using the red flour beetle, Tribolium castaneum (Herbst), as a relevant model. Transcription of focal genes thought to be involved in protection against ozone, and repair of cellular damage caused by ozone exposure, was studied together with the composition of storage lipid fatty acids and membrane phospholipid fatty acids in order to detect lipid peroxidation. Contrary to expectations, transcription ...

2011-01-01

153

Association and expression study of synapsin III and schizophrenia

British Library Electronic Table of Contents (United Kingdom)

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter release and in synaptogenesis, suggesting a potential role in several neuropsychiatric diseases. The human SYN3 gene is located on chromosome 22q12-13, a candidate region implicated in previous linkage studies of schizophrenia. However, association studies of SYN3 and schizophrenia have produced inconsistent results. In this study, four SYN3 SNPs (rs133945 (-631 C>G), rs133946 (-196 G>A), rs9862 and rs1056484) were tested in three sets of totally 3759 samples that comprise 655 affected subjects and 626 controls in the Irish Case-Control Study of Schizophrenia (ICCSS), 1350 samples incorporating 273 pedigrees in the Irish Study of High D...

2009-01-01

154

Transcriptional Profiling Analysis of the Global Regulator NorG, a GntR-Like Protein of Staphylococcus aureus.

Computational identification of developmental enhancers:conservation and function of transcription factor binding-site clustersin drosophila melanogaster and drosophila psedoobscura

The GntR-like protein NorG has been shown to affect Staphylococcus aureus genes involved in the resistance to quinolones and ?-lactams, such as those encoding the NorB and AbcA transporters. To identify the target genes regulated by NorG, we carried out transcriptional profiling assays using S. aureus RN6390 and its isogenic norG::cat mutant. Our data showed that NorG positively affected the transcription of global regulators mgrA, arlS, and sarZ. The three putative drug efflux pump genes most positively affected by NorG were the NorB efflux pump (5.1-fold), the MmpL-like protein SACOL2566 (5.2-fold), and the BcrA-like drug transporter SACOL2525 (5.7-fold). The S. aureus predicted MmpL protein showed 53% homology with the MmpL lipid transporter of Mycobacterium tuberculosis, and the putative SACOL2525 protein showed 87% homology with the bacitracin drug transporter BcrA of Staphylococcus hominis. Two ...

2011-09-01

155

[Molecular cloning and expression of an isotoxin gene, alpha-bungarotoxin, from Bungarus multicinctus].

Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. Results We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated ...

2004-08-06

156

Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato

Abstract: Snake venom contains a number of small proteins,enzymes and other components,which displays a broad spectrum of biological activities. With the ability of specifically binding on acetylcholine acceptor, alpha-bungarotoxins are not only useful molecular probes in investigating the mechanism of neural signal transmission, but also potential pharmic preparations for neural disease treatment. In current research,cDNAs of Bungarus multicinutus venom gland were synthesized using SMART cDNA amplification kit and then, alpha-bungarotoxin genes were cloned and sequenced. Total of 20 clones were sequenced representing 14 isotoxin mRNAs of alpha-bungarotoxins. Among those clones, a novel isotoxin gene was subcloned into two expression plasmids, alpha-BgTX/pQE30a and alpha-BgTX/pGEX-4T-1, and transformed into E. coli. After inducing with IPTG, fused protein of GST-alpha-BgTX was successfully expressed at level of 30% gross ...

2005-07-01

157

British Library Electronic Table of Contents (United Kingdom)

Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry chc ) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples, were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially harboring all five resistance genes were selected from 96 hybrid plants approximately 5?h after DNA extraction, which is a third of the operation time compa...

2011-01-01

158

Inherited partial direct duplication of 11q: First report and possible association with a midline developmental field defect

Energy management, real estate management, and facility management; Energiemanagement in der Immobilienverwaltung

A 36-year-old woman underwent amniocentesis for advanced maternal age. The fetal karyotype had an extra dark staining G band on the long arm of chromosome 11 with no other identifiable abnormalities. FISH studies using a chromosome 11 paint probe confirmed the origin of the extra band. The abnormality was identified as a partial duplication of 11q: 46,XX dir dup (11)(q13.5q21) or (q21q23.1). The specific duplicated band could not be identified with certainty. Detailed fetal sonograms were normal. Family studies revealed the identical duplication in the mother but normal karyotypes in both maternal grandparents. The mother had strabismus and a short tongue frenulum which required surgical correction. Menses occurred late in adolescence and complete development of secondary sexual characteristics was delayed until adulthood. An infertility evaluation revealed duplication of the uterus, cervix, and vagina. An evaluation for ...

1994-09-01

159

Tuning and controlling gene expression noise in synthetic gene networks

The publication presents an outline of the subject of energy management and attempts to test the hypotheses that targeted knowledge management may result in new developments and potentials, and if these are at all desirable. On this basis, a knowledge management system will be developed that may effectively promote developments and potentials in the field of energy management. The tools and fundamentals of energy management are looked into, and the given situation and desired goals are identified by means of an enquiry. This is a scientific study that aims at providing empirical information on the situation and views on the subject of energy management. The analysis, which is supported by an enquiry sheet, is to provide a basis for testing the assumed hypothesis. Further, the potential acceptance and utilization potential of decisive knowledge management in the facility and real ...

2010-07-01

160

Biological Research for Radiation Protection

Synthetic gene networks can be used to control gene expression and cellular phenotypes in a variety of applications. In many instances, however, such networks can behave unreliably due to gene expression...Full Text Available

2010-05-01

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161

Regulation of the pT181 encoded tetracycline resistance gene in Straphylococcus aureus

The work scope of 'Biological Research for the Radiation Protection' had contained the research about ornithine decarboxylase and its controlling proteins, thioredoxin, peroxiredoxin, S-adenosymethionine decarboxylase, and glutamate decarboxylase 67KD effect on the cell death triggered ionizing radiation and H_2O_2(toxic agents). In this study, to elucidate the role of these proteins in the ionizing radiation (or H_2O_2)-induced apoptotic cell death, we utilized sensesed (or antisensed) cells, which overexpress (or down-regulate) RNAs associated with these proteins biosynthesis, and investigated the effects of these genes on the cytotoxicity caused by ionizing radiation and H_2O_2(or paraquat). We also investigated whether genisteine(or thiamine) may enhance the cytotoxic efficacy of tumor cells caused by ionizing radiation (may enhance the preventing effect radiation or paraquat-induced damage) because such compounds are able to potentiate the ...

162

Sensitivity analysis: Interaction of DOE SNF and packaging materials

pT181 is a naturally-occurring 4437 basepair (bp) plasmid isolated from Staphylococcus aureus which encodes inducible resistance to tetracycline (Tc). The DNA sequence data has identified three open reading frames (ORFs). The largest ORF B, has been found to be responsible for the Tc resistance phenotype of pT181. Since most Tc resistance systems appear to be regulated by an effector protein and a repressor protein, several Bal 31 deletion mutants of pT181 were constructed and analyzed in an effort to identify the elements involved in Tc resistance. Two transcomplementing groups of mutants were identified within the tet gene. The mechanism of Tc resistance was studied by assaying the accumulation of (7-/sup 3/H) Tc by Tc sensitive cells, and uninduced and induced pT181-containing cells. A sharp decrease in accumulation of the drug after an initial increase was observed in Tc induced pT181-containing ...

1986-01-01

163

Materials for air turbines in wave power devices. A generic study

A sensitivity analysis was conducted to evaluate the technical issues pertaining to possible destructive interactions between spent nuclear fuels (SNFs) and the stainless steel canisters. When issues are identified through such an analysis, they provide the technical basis for answering what if questions and, if needed, for conducting additional analyses, testing, or other efforts to resolve them in order to base the licensing on solid technical grounds. The analysis reported herein systematically assessed the chemical and physical properties and the potential interactions of the materials that comprise typical US Department of Energy (DOE) SNFs and the stainless steel canisters in which they will be stored, transported, and placed in a geologic repository for final disposition. The primary focus in each step of the analysis was to identify any possible phenomena that could potentially compromise the ...

1999-06-06

164

Identifying the global potential for baobab tree cultivation using ecological niche modelling

Wave energy device teams have identified three varieties of air turbine as potentially applicable to wave energy devices. These are: conventional axial turbines; Wells, or self-rectifying, axial turbines and Francis turbines. This report examines the constructional requirements of these devices with regard to mechanical, environmental and manufacturing considerations. It is concluded that the major benefit of optimum material selection will be reduced manufacturing costs rather than enhanced turbine performance. A methodology of material selection has been established and candidate materials have been listed for the major components of each turbine type. Comparative costs for alternative materials are included, from which significant, potential economies have been identified. Recommendations are made aimed at achieving optimum material usage in the proposed turbines.

1981-01-01

165

British Library Electronic Table of Contents (United Kingdom)

The benefits provided by underutilised fruit tree species such as baobab (Adansonia digitata L.) in combating increasing malnutrition and poverty become more apparent as awareness grows regarding concerns of climate change and food security. Due to its multiple uses, its high nutritional and medicinal value, drought tolerance and relatively easy cultivation, baobab has been identified as one of the most important edible forest trees to be conserved, domesticated and valued in Africa. In order to contribute towards the cultivation of the species, suitability of sites in Africa and worldwide was evaluated for potential cultivation using species? locality data and spatial environmental data in MAXENT modelling framework. A total of 450 geo-referenced records of the baobab tree were assembled ...

2010-01-01

166

Identification of prostate cancer antigens by automated high-throughput filter immunoscreening

British Library Electronic Table of Contents (United Kingdom)

There is a need for earlier and more accurate cancer diagnostics as well as new targets for cancer immunotherapy. To this end, it is important to identify sets of tumour antigens specific for different cancer forms. Several methods that identify potential tumour antigens in an arrayed and high-throughput format have been developed during the last years of SEREX (serological identification of antigens by recombinant expression cloning) related research. Such techniques may hold the potential to describe the complete immunogenic part of the cancer proteome, also called the cancer immunoproteome.We have developed a powerful platform for automated serological high-throughput filter screening of tumour cDNA libraries. The screening format of this method is 18,000 single cDNAs clones, which is s...

2008-01-01

167

Application of probabilistic methods to accident analysis at waste management facilities

Characterization of the mannan synthase promoter from guar (Cyamopsis tetragonoloba)

Probabilistic risk assessment is a technique used to systematically analyze complex technical systems, such as nuclear waste management facilities, in order to identify and measure their public health, environmental, and economic risks. Probabilistic techniques have been utilized at the Waste Isolation Pilot Plant (WIPP) near Carlsbad, New Mexico, to evaluate the probability of a catastrophic waste hoist accident. A probability model was developed to represent the hoisting system, and fault trees were constructed to identify potential sequences of events that could result in a hoist accident. Quantification of the fault trees using statistics compiled by the Mine Safety and Health Administration (MSHA) indicated that the annual probability of a catastrophic hoist accident at WIPP is less than one in 60 million. This result allowed classification of a catastrophic hoist accident as ''not credible'' at WIPP per DOE ...

168

British Library Electronic Table of Contents (United Kingdom)

Guar seed gum, consisting primarily of a high molecular weight galactomannan, is the most cost effective natural thickener, having broad applications in the food, cosmetics, paper, pharmaceutical and petroleum industries. The properties of the polymer can potentially be enhanced by genetic modification. Development of suitable endosperm-specific promoters for use in guar is desirable for metabolic engineering of the seed gum. A ~1.6?kb guar mannan synthase (MS) promoter region has been isolated. The MS promoter sequence was fused with the GUS reporter gene and overexpressed in the heterologous species alfalfa (Medicago sativa). The potential strength and specificity of the MS promoter was compared with those of the constitutive 35S promoter and the seed specific ?-phaseolin promoter. Quant...

2011-01-01

169

Missense mutations in the growth hormone receptor dimerization region in Laron syndrome

Underground electric-power transmission-system environmental impact assessment

Laron syndrome (LS) is an autosomal recessively inherited condition characterized by insensitivity to endogenous and exogenous GH. Affected individuals have severe episodes and other characteristic features. GH receptor gene mutations are present in all affected individuals in whom molecular studies have been reported. The GH receptor is a plasma membrane-spanning protein in which the extracellular domain binds circulating GH and the intracellular domain interacts with the JAK-2 kinase and possibly other intracellular signaling molecules. GH receptor dimerization occurs on GH binding and is thought to be required for normal signal transduction. We have studied the GH receptor genes of four unrelated individuals affected with LS from the United States, Italy, Saudi Arabia, and India. We have identified four different missense mutations that alter consecutive amino acids 152 to 155 in or near the dimerization domain of the GH ...

1994-09-01

170

Thermal-receiver designs for line-focus solar collectors

The US Department of Energy, Division of Electric Energy Systems, has undertaken to identify the environmental issues and potential impacts associated with the installation of underground electric power transmission systems. This study reports the results of investigations into the advanced cable technologies being considered for future underground applications, as part of the development oriented research program of the Division of Electric Energy Systems. While the technology involves a high level of sophistication, there are relatively few impacts to the environment that are potentially significant, and of these none are inherently non-mitigable. Route planning, system design, and methods of construction and accident response can be pursued in order to minimize impacts where strict constraints are appropriate.

1982-03-01

171

The identification of the 1/2"+[660] proton orbitals at high spins in rare-earth nuclei

Considerable potential exists for increasing the annual efficiency of line-focus collectors by reducing receiver heat losses. Five alternate receiver designs are compared with a standard design. The comparison is based on annual collector-field efficiency using optimized receiver designs. Of the receivers studied, the all-glass design and the cavity design provide the greatest increases in efficiency. Average fluid temperatures range from 200/sup 0/ to 450/sup 0/C. As a guide for further study, areas of uncertainty and potential problems are identified.

1982-02-01

172

Mine-associated wetlands as avian habitat

Decay sequences based on the 1/2"+[660] proton orbital have been identified in "1"7"1Ta and "1"7"7Re based on spin, parity, and large alignment. This decay sequence is observed higher in energy than predicted in cranking calculations based on modified oscillator potentials. Similarly known 1/2"-[541] decay sequences in these and other neighbouring isotopes are observed lower in energy than predicted. A reduction in the strength of the spin-orbit potential for protons is suggested as a solution to these problem. (orig.).

173

Identification of the 1/2/sup +/(660) proton orbitals at high spins in rare-earth nuclei

Surveys for interior wetland birds at mine-associated emergent wetlands on coal surface mines in southern Illinois detected one state threatened and two state endangered species. Breeding by least bittern (Ixobrychus exilis) and common moorhen (Gallinula chloropus) was confirmed. Regional assessment of potential wetland bird habitat south of Illinois Interstate 64 identified a total of 8,109 ha of emergent stable water wetlands; 10% were associated with mining. Mine-associated wetlands with persistent hydrology and large expanses of emergent vegetation provide habitat that could potentially compensate for loss of natural wetlands in Illinois.

1998-06-01

174

Homology analyses of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast

Decay sequences based on the 1/2/sup +/(660) proton orbital have been identified in /sup 171/Ta and /sup 177/Re based on spin, parity, and large alignment. This decay sequence is observed higher in energy than predicted in cranking calculations based on modified oscillator potentials. Similarly known 1/2/sup -/(541) decay sequences in these and other neighbouring isotopes are observed lower in energy than predicted. A reduction in the strength of the spin-orbit potential for protons is suggested as a solution to these problem.

1983-12-08

175

Remote measurement of methane in coal mines: a review

Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases were carried out. The amino acid sequences of the chicken and rat enzymes are 67% identical. If conservative substitutions are allowed, 78% of the amino acids are matched. A region of low homologies exists between the functional domains, in particular around amino acid residues 1059-1264 of the chicken enzyme. Homologies between the active sites of chicken and rat and of chicken and yeast enzymes have been analyzed by an alignment method. A high degree of homology exists between the active sites of the chicken and rat enzymes. However, the chicken and yeast enzymes show a lower degree of homology. The DADPH-binding dinucleotide folds of the {beta}-ketoacyl reductase and the enoyl reductase sites were identified by comparison with a known consensus sequence for the DADP- and FAD-binding dinucleotide folds. The active sites of all of the enzymes are primarily in ...

1989-11-01

176

Overview of cleaner production as a result of clean technology research in Vietnam

The authors identify a need for a device which can remotely measure methane in coal mines. Similar techniques which have been used to measure atmospheric pollutants are discussed. These techniques include adsorption, Raman scattering and fluorescence. While differential adsorption and Raman scattering techniques have potential there are a number of problems to be resolved before they could be applied effectively. It is felt that an area which may provide suitable technology sometime in the future is in the field of laser sources. 6 refs., 4 tabs.

1987-02-01

177

Long-term chemical carcinogenesis experiments for identifying potential human cancer hazards: collective database of the National Cancer Institute and National Toxicology Program (1976-1991).

Vietnam is beginning its industrialization process, and it currently lacks the capital necessary for expensive pollution control and treatment systems. Pollution strategies are critical for the country. This study evaluates the present status of industry and the pollution problems associated with industrial development in Vietnam. The purpose of the study is to identify sectors that may have potential for promoting cleaner production practices. 4 refs.

1996-12-31

178

Implications of biodiversity of short rotation coppice

The carcinogenicity database used for this paper originated in the late 1960s by the National Cancer Institute (NCI) and since 1978 has been continued and made more comprehensive by the National Toxicology...Full Text Available

1991-12-01

179

Environmental Research Database

DescriptionFossil fuels have a detrimental effect on the environment. They lead to the increase in atmospheric carbon dioxide which has been documented over the last 150 years. In contrast, some sources of renewable energy are near carbon neutral. Renewable energy produced from biomass constitutes such a type of energy and so has many potential advantages. The Energy White Paper (DTI et al., 2003) identifies bioenergy as an important means of meeting the Government's energy and environment objectives, in [continued...

2009-01-31

180

Genome-Wide Association Study SNPs in the Human Genome Diversity Project Populations: Does Selection Affect Unlinked SNPs with Shared Trait Associations?

Genetic and Structural Insights into the Dissemination Potential of the Extremely Broad-Spectrum Class A ?-Lactamase KPC-2 Identified in an Escherichia coli Strain and an Enterobacter cloacae Strain Isolated from the Same Patient in France?

Genome-wide association studies (GWAS) have identified more than 2,000 trait-SNP associations, and the number continues to increase. GWAS have focused on traits with potential consequences for human...Full Text Available

2011-01-01

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181

Generic design framework pile foundations (fixed steel structures)

Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum...Full Text Available

2008-10-01

182

AEC syndrome - Genetics Home Reference

To assist in attaining the HSE's objective of providing perspective and focus for Safety Case Assessment, a generic model of the structural design process for the foundations of pile supported fixed steel structures has been developed. Interfaces between the foundation design team and other stakeholders have been identified and assessments made of both the magnitude and the potential significance of the uncertainties associated with key elements of the design process. (author)

2001-07-01

183

Prediction of the naturalisation potential and weediness risk of transgenic cotton in Australia

What genes are related to AEC syndrome? AEC syndrome is caused by mutations in the TP63 gene. This gene provides instructions for making a protein known as p63, which plays an...

2011-10-15

184

British Library Electronic Table of Contents (United Kingdom)

Climex climate-matching and inferential-modelling was used to examine the naturalisation potential of genetically modified and non-transgenic Upland Cotton (Gossypium hirsutum variety hirsutum) in Australia north of latitude 22degreeS. The Climate-Match (CM) function was used to identify locations in north-east Australia where climate matches (CM Index>0.7) the experimental sites of Eastick [Eastick, R., 2002. The Potential Weediness of Transgenic Cotton in Northern Australia. Northern Territory Department of Business, Industry and Resource Development Technical Bulletin No. 305 (Internet resource: http://cotton.pi.csiro.au/Assets/PDFFiles/TB3051.pdf)] in north-west Australia. Most of Australia north of 22degreeS had climate matching one or more of the sites of Eastick (2002), locations wh...

2007-01-01

185

An old tool with potential new uses: return on investment

British Library Electronic Table of Contents (United Kingdom)

Purpose - The purpose of this paper is to examine potential new uses of return on investment (ROI) in alternative methods for library assessment and valuation. Design/methodology/approach - Uses examples of recent uses of ROI by libraries to identify potential new applications of ROI in library assessment and valuation. Findings - ROI investment has historically been used by libraries for the assessment of past performance of library organizations in a reactive/defensive nature or for assessing the value of customer services. ROI has not been widely used as a proactive assessment tool, to introspectively assess administrative or other internal services, or as an offensive assessment and valuation tool, especially for intangible performance assessment and valuation. Originality/value - New ...

2007-01-01

186

Accelerated aging corrosion tests for buried metal structures

The Notch ligand Delta-like 1 integrates inputs from TGFbeta/Activin and Wnt pathways

It is necessary to identify those measurable soil parameters which dictate the severity of the corrosion problem for coated irons and steels. When this is done, meaningful accelerated aging tests can be designed to validate the reduced corrosion rate for the planned coating. The following discussion introduces the important concepts and measurement parameters in the (a) design of accelerated aging tests, and (b) evaluation of the planned installation site for corrosion potential. Certain combinations of soil oxidation reduction potential (ORP) or electric potential (Eh), acidity (pH), fertilizer, water table, and soil hydraulic conductivity can result in severe corrosion of buried steel/iron vessels. If there are power lines nearby, additional loss of vessel material to the soil will occur. These factors are discussed.

1996-10-01

187

British Library Electronic Table of Contents (United Kingdom)

Unlike the well-characterized nuclear function of the Notch intracellular domain, it has been difficult to identify a nuclear role for the ligands of Notch. Here we provide evidence for the nuclear function of the Notch ligand Delta-like 1 in colon cancer (CC) cells exposed to butyrate. We demonstrate that the intracellular domain of Delta-like 1 (Dll1icd) augments the activity of Wnt signaling-dependent reporters and that of the promoter of the connective tissue growth factor (CTGF) gene. Data suggest that Dll1icd upregulates CTGF promoter activity through both direct and indirect mechanisms. The direct mechanism is supported by co-immunoprecipitation of endogenous Smad2/3 proteins and Dll1 and by chromatin immunoprecipitation analyses that revealed the occupancy of Dll1icd on CTGF promot...

2011-01-01

188

Proteomic analysis of sericin in Bombyx mori cocoons

British Library Electronic Table of Contents (United Kingdom)

Cocoon sericin plays an important role in the reeling of silk and serves as a valuable biomaterial in the field of biomedicine, skincare, and food industries; however, knowledge about cocoon sericin proteins has been limited. For a comprehensive study on sericin, cocoons of eight varieties of silkworm of different geographic origin and with varied cocoon color were analyzed utilizing proteomics and bioinformatics approaches. The electrophoresis pattern demonstrated some common protein bands for all silkworm varieties and distinctive protein bands for some of those examined in the present study. The Ser2 protein, a new Ser3 protein, and four other novel sericin proteins were identified in cocoons for the first time. Products of both Ser1 and Ser3 genes appear to be ubiquitous in the cocoon ...

2011-01-01

189

Identification of a functional nuclear export signal in the green fluorescent protein asFP499

Identification of AFAP1L1 as a prognostic marker for spindle cell sarcomas

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

190

British Library Electronic Table of Contents (United Kingdom)

Spindle cell sarcomas consist of tumors with different biological features, of which distant metastasis is the most ominous sign for a poor prognosis. However, metastasis is difficult to predict on the basis of current histopathological analyses. We have identified actin filament-associated protein 1-like 1 (AFAP1L1) as a candidate for a metastasis-predicting marker from the gene expression profiles of 65 spindle cell sarcomas. A multivariate analysis determined that AFAP1L1 was an independent factor for predicting the occurrence of distant metastasis (P=0.0001), which was further confirmed in another set of 41 tumors by a quantitative mRNA expression analysis. Immunohistochemical staining using paraffin-embedded tumor tissues revealed that the metastasis-free rate was significantly better...

2011-01-01

191

Genome-wide association study identifies variants in the CFH region associated with host susceptibility to meningococcal disease

British Library Electronic Table of Contents (United Kingdom)

Meningococcal disease is an infection caused by Neisseria meningitidis. Genetic factors contribute to host susceptibility and progression to disease, but the genes responsible for disease development are largely unknown. We report here a genome-wide association study for host susceptibility to meningococcal disease using 475 individuals with meningococcal disease (cases) and 4,703 population controls from the UK. We performed, in Western European and South European cohorts (consisting of 968 cases and 1,376 controls), two replication studies for the most significant SNPs. A cluster of complement factor SNPs replicated independently in both cohorts, including SNPs within complement factor H (CFH) (rs1065489 (p.936D

2010-01-01

192

Evolutionary implications of morphogenesis and molecular patterning of the blind gut in the planarian Schmidtea polychroa

British Library Electronic Table of Contents (United Kingdom)

The formation of a through-gut was a key innovation in the evolution of metazoans. There is still controversy regarding the origin of the anus and how it may have been either gained or lost during evolution in different bilaterian taxa. Thus, the study of groups with a blind gut is of great importance for understanding the evolution of this organ system. Here, we describe the morphogenesis and molecular patterning of the blind gut in the sexual triclad Schmidtea polychroa. We identify and analyze the expression of goosecoid, commonly associated with the foregut, and the GATA, ParaHox and T-box genes, members of which commonly are associated with gut regionalization. We show that GATA456a is expressed in the blind gut of triclads, while GATA456b is localized in dorsal parenchymal cells. Goo...

2011-01-01

193

Erythroid Differentiation Regulator 1, an Interleukin 18-Regulated Gene, Acts as a Metastasis Suppressor in Melanoma

British Library Electronic Table of Contents (United Kingdom)

Erythroid differentiation regulator (Erdr1) was first discovered in mouse leukemia cell lines and functions as a stress-related survival factor. This study investigated whether Erdr1 regulates murine melanoma progression, as well as the mechanism involved in Erdr1-regulated metastasis. The expression of Erdr1 is negatively correlated with IL-18 expression, which has a pro-cancer effect in melanoma. To study the role of Erdr1 as an anti-cancer factor, cell migration, invasion, and proliferation were measured. Erdr1 overexpression markedly inhibited the level of cell migration, invasion, and proliferation in B16F10 cells in vitro. In addition, Erdr1 overexpression significantly suppressed melanoma lung colonization, metastasis, and tumor growth in vivo. To identify the factors involved in Er...

2011-01-01

194

Cell turnover and gene activities in sheep mammary glands prior to lambing to involution

British Library Electronic Table of Contents (United Kingdom)

Mammary glands are special tissue characterized by proliferation of the epithelium, during puberty and pregnancy and by programmed cell death, during involution. In this study, apoptosis was identified by TUNEL staining and then related to cell proliferation, as determined by Ki-67 staining. The apoptotic index was at its highest at 8 days of involution, whereas the proliferation index was at its highest during lactation. Caspase-3 was immunolocalised only in mast cells and along the basal membrane in the mammary tissue at -10 days from lambing, 150 days of lactation and at 8 days of involution. This finding could indicate that caspase-3 is not involved in sheep mammary gland apoptosis, but that other proteins - such as apoptosis inducing factor (AIF) - can trigger apoptosis, through the m...

2009-01-01

195

Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root.

Design of novel polysaccharidic nanostructures for gene delivery

Glycyrrhiza glabra root is one of the common traditional Chinese medicines and used as flavoring and sweetening agents for tobaccos, chewing gums, candies, toothpaste and beverages. While glycyrrhizin is one of the main components in the extract of G. glabra root and has been characterized, the other components have not been well characterized. The mechanism of growth activation of breast cancer MCF-7 cells, including the activation of Erk1/2 and Akt, and the transcriptional regulation of estrogen-responsive genes, was examined by means of sulforhodamine B, luciferase reporter gene, real-time RT-PCR and Western blotting assays after the induction of the cells with the extract of G. glabra root. The extract has similar activity to that induced by 17beta-estradiol (E(2)), although glycyrrhizin did not show such an activity. Moreover, the estrogen receptor alpha-dependent neurite outgrowth induced by the extract was similar to that by E(2), ...

2007-06-24

196

Toxicological benchmarks for screening contaminants of potential concern for effects on freshwater biota

The goal of the present work was to develop a new synthetic nanosystem for gene delivery. For this purpose, we chose two polysaccharides, hyaluronic acid (HA) and chitosan (CS), as the main components of the nanocarrier. Nanoparticles with different hyaluronate:chitosan (HA:CS) mass ratios (0.5:1 and 1:1) and different polymer molecular weights (hyaluronate 170 (HA) or <10 kDa (HAO) and chitosan 125 (CS) or 10-12 (CSO) kDa) could be obtained using an ionic crosslinking method. These nanoparticles were loaded with pDNA and characterized for their size, zeta potential and pDNA association efficiency. Moreover, their toxicity and ability to transfect the model plasmid pEGFP-C1 were evaluated in the cell line HEK 293, as well as their intracellular fate. The results showed that HA:CS nanoparticles have a small size in the range of 110-230 nm, a positive zeta potential of +10 to +32 mV and a very high pDNA association ...

2008-02-20

197

Hydrocarbon potential of offshore South Florida basin

An important early step in the assessment of ecological risks at contaminated sites is the screening of chemicals detected on the site to identify those that constitute a potential risk. Part of this screening process is the comparison of measured ambient concentrations to concentrations that are believed to be nonhazardous, termed benchmarks. This article discusses 13 methods by which benchmarks may be derived for aquatic biota and presents benchmarks for 105 chemicals. It then compares them with respect to their sensitivity, availability, magnitude relative to background concentrations, and conceptual bases. This compilation is limited to chemicals that have been detected on the US Department of Energy's Oak Ridge Reservation (ORR) and to benchmarks derived from studies of toxic effects on freshwater organisms. The list of chemicals includes 45 metals and 56 industrial organic chemicals but only four pesticides. Although some individual ...

198

Gene discovery in the Acanthamoeba castellanii genome

The results of an extensive, detailed geologic and geophysical evaluation of the offshore South Florida basin show this area to have considerable hydrocarbon exploration potential. Geophysical mapping on six key horizons identified numerous structural closures at Dollar Bay through Bone Island mapping levels. Geologic evaluation indicated at least four viable potential reservoir horizons, including the Sunniland formation - the main producing unit onshore in what is called the Sunniland trend. Mature, organic rich carbonate source rocks are found in several stratigraphic intervals throughout the basin, and extensively developed seals consisting of micritic limestones and anhydrites are also common in the stratigraphic section. All the critical factors for hydrocarbon accumulation - source, reservoir, trap, and seal - appear to be present in the offshore Pulley ridge area of the South Florida basin. Using high-resolution ...

1986-05-01

199

Alteration of alpha 1 Na+,K(+)-ATPase "8"6Rb"+ influx by a single amino acid substitution

Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria. Acanthamoeba castellanii is also an opportunistic pathogen of clinical interest, responsible for several distinct diseases in humans. In order to provide a genomic platform for the study of this ubiquitous and important protist, we generated a sequence survey of approximately 0.5 x coverage of the genome. The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A. castellanii to inhabit adversity of environments. Alginate lyase may provide access to bacteria within biofilms by breaking down the biofilm matrix, and polyhydroxybutyrate depolymerase may facilitate utilization of the bacterial storage compound polyhydroxybutyrate as a food source. Enzymes for the synthesis and breakdown of cellulose were ...

2005-08-01

200

tA Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta

The sodium- and potassium-dependent adenosine triphosphatase (Na+,K(+)-ATPase) maintains the transmembrane Na+ gradient to which is coupled all active cellular transport systems. The R and S alleles of the gene encoding the Na+,K(+)-ATPase alpha 1 subunit isoform were identified in Dahl salt-resistant (DR) and Dahl salt-sensitive (DS) rats, respectively. Characterization of the S allele-specific Na+,K(+)-ATPase alpha 1 complementary DNA identified a leucine substitution of glutamine at position 276. This mutation alters the hydropathy profile of a region in proximity to T3(Na), the trypsin-sensitive site that is only detected in the presence of Na+. This mutation causes a decrease in the rubidium-86 influx of S allele-specific sodium pumps, thus marking a domain in the Na+,K(+)-ATPase alpha subunit important for K+ transport, and supporting the hypothesis of a putative role of these pumps in hypertension.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

201

Unraveling gene regulatory networks from time-resolved gene expression data -- a measures comparison study

The rice blast resistance (R) gene Pi-ta mediates gene-for-gene resistance against strains of the fungus Magnaporthe grisea that express avirulent...Full Text Available

2000-11-01

202

The ovalbumin gene family: complete sequence and structure of the Y gene.

BackgroundInferring regulatory interactions between genes from transcriptomics time-resolved data, yielding reverse engineered gene regulatory networks, is of paramount importance...Full Text Available

203

NUCLEIC ACID CONTAINING ELEMENT FOR TRANSFERRING FOREIGN GENE

The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin...Full Text Available

1982-07-24

204

J-STORE (Japan)

Full Text Available

2009-02-27

205

Duplication and Diversification of the Hypoxia-Inducible IGFBP-1 Gene in Zebrafish

Detecting microRNA activity from gene expression data

BackgroundGene duplication is the primary force of new gene evolution. Deciphering whether a pair of duplicated genes has evolved divergent functions is often challenging. The zebrafish...Full Text Available

206

Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family.

BackgroundMicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression by binding to the messenger RNA (mRNA) of protein coding genes. They control gene expression by either...Full Text Available

207

Characterization of Two New Genes, amoR and amoD, in the amo Operon of the Marine Ammonia Oxidizer Nitrosococcus oceani ATCC 19707?

The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase...Full Text Available

1985-07-01

208

A statistical framework for modeling gene expression using chromatin features and application to modENCODE datasets

Molecular analysis of the amo gene cluster in Nitrosococcus oceani revealed that it consists of five genes, instead of the three known genes, amoCAB....Full Text Available

2008-01-01

209

A faster pedigree-based generalized multifactor dimensionality reduction method for detecting gene-gene interactions

We develop a statistical framework to study the relationship between chromatin features and gene expression. This can be used to predict gene expression of protein coding genes, as well as microRNAs....Full Text Available

2011-01-01

210

The mitochondrial genome of the entomophagous endoparasite Xenosvesparum (Insecta: Strepsiptera)

We proposed a faster pedigree-based generalized multifactor dimensionality reduction algorithm, called PedG-MDR II (PII), to detect gene-gene interactions underlying complex traits. Inherited...Full Text Available

2011-01-01

211

The complete mitochondrial genome of Atelura formicaria (Hexapoda: Zygentoma) and the phylogenetic relationships of basal insects.

In this study, the nearly complete sequence (14,519 bp) of the mitochondrial DNA (mtDNA) of the entomophagous endoparasite Xenos vesparum (Insecta: Strepsiptera) is described. All protein coding genes (PCGs) are in the arrangement known to be ancestral for insects, but three tRNA genes (trnA, trnS(gcu), and trnL(uag)) have transposed to derived positions and there are three tandem copies of trnH, each of which is potentially functional. All of these rearrangements except for that of trnL(uag) is within the short span between nad3 and nad4 and there are numerous blocks of unassignable sequence in this region, perhaps as remnants of larger scale predisposing rearrangements. X. vesparum mtDNA nucleotide composition is strongly biased toward As and Ts, as is typical for insect mtDNAs. There is also significant strand skew in the distribution of these nucleotides, with the J-strand being richer in A than T and in C than G, and ...

2005-12-01

212

Regulatory role of neuron-restrictive silencing factor in expression of TRPC1

In this study, the complete sequence of the mitochondrial DNA (mtDNA) of Atelura formicaria (Hexapoda: Zygentoma) is described. The molecule is 15.205 bp in length and it is the third complete mt genome sequenced from the Zygentoma. The genome organization conforms with the putative ancestral insect gene arrangement. All protein coding genes use standard initiation codons (methionine and isoleucine). The exception is nad4 that starts with GTG, a codon used for this purpose in other insect species. A peculiar strand skew bias is observed, given that the PCGs encoded on the J-strand contain more thymines than adenines and more cytosines than guanines. This trend in nucleotide composition has been observed also in the "firebrat" Thermobia domestica (Zygentoma, Lepismatidae), but differs from that of the majority of hexapod species, including Tricholepidion gertschi (Zygentoma, Lepidotrichidae), where adenines and cytosines outnumber thymines and ...

2009-03-11

213

A versatile and potentially general approach to the targeting of specific cell types by retroviruses: Application to the infection of human cells by means of major histocompatibility complex class I and class II antigens by mouse ecotropic murine leukemia virus-derived viruses

Neuron-restrictive silencer factor (NRSF) binds its consensus element to repress the transcription of various genes. The dominant-negative form (dnNRSF) has a hypertrophic effect on cardiogenesis through an unidentified mechanism. We examined the involvement of transient receptor potential (TRP) channel proteins, using transgenic mice overexpressing dnNRSF (dnNRSF mice). Electrophoretic mobility-shift assays revealed an interaction between NRSF and a neuron-restrictive silencer element-like sequence in intron 4 of TRPC1 genomic DNA. According to RT-PCR and Western analyses, TRPC1 was up-regulated in dnNRSF mouse heart. Transient overexpression of TRPC1 in HEK 293T cells increased the activity of the nuclear factor in activated T cells (NFAT) promoter and stimulated store-operated Ca"2"+ channel (SOCC)-mediated Ca"2"+ entry. Transfection of TRPC1 into primary cardiomyocytes increased NFAT activity, indicating a major role for TRPC1 in NFAT ...

2006-12-22

214

Functional characterization of a CCCH type zinc-finger protein gene OsZF2 by ectopic overexpression of the gene in rice

A technique for delivering genes carried by recombinant retroviruses into specific cell types could have numerous applications in oncology, developmental biology, and gene therapy. As a first step toward this remote goal the authors designed a procedure allowing in vitro cell targeting by retroviruses. Biotinylated antibodies against the viral envelope protein on one side, and against specific cell membrane markers on the other side, were bridged by streptavidin and used to link the virus to the host. The method was successfully used to infect human cells with ecotropic murine retroviruses by means of major histocompatibility complex class I and class II antigens and appears easily adaptable to other cell, membrane markers. Moreover, the sequential protocol they design, although allowing infection of human cells, requires less stringent safety constraints than would handling of amphotropic virus stocks.

1989-12-01

215

Protein-coding and non-coding gene expression analysis in differentiating human keratinocytes using a three-dimensional epidermal equivalent.

Full Text Available

2009-03-01

216

Association of attention-deficit disorder and the dopamine transporter gene

The epidermal compartment is complex and organized into several strata composed of keratinocytes (KCs), including basal, spinous, granular, and cornified layers. The continuous process of self-renewal and barrier formation is dependent on a homeostatic balance achieved amongst KCs involving proliferation, differentiation, and cell death. To determine genes responsible for initiating and maintaining a cornified epidermis, organotypic cultures comprised entirely of stratified KCs creating epidermal equivalents (EE) were raised from a submerged state to an air/liquid (A/L) interface. Compared to the array profile of submerged cultures containing KCs predominantly in a proliferative (relatively undifferentiated) state, EEs raised to an A/L interface displayed a remarkably consistent and distinct profile of mRNAs. Cultures lifted to an A/L interface triggered the induction of gene groups that regulate proliferation, differentiation, and cell death. ...

2010-05-25

217

Aarskog-Scott syndrome: Clinical update and report of nine novel mutations of the FGD1 gene

Attention-deficit hyperactivity disorder (ADHD) has been shown to be familial and heritable, in previous studies. As with most psychiatric disorders, examination of pedigrees has not revealed a consistent Mendelian mode of transmission. The response of ADHD patients to medications that inhibit the dopamine transporter, including methylphenidate, amphetamine, pemoline, and bupropion, led us to consider the dopamine transporter as a primary candidate gene for ADHD. To avoid effects of population stratification and to avoid the problem of classification of relatives with other psychiatric disorders as affected or unaffected, we used the haplotype-based haplotype relative risk (HHRR) method to test for association between a VNTR polymorphism at the dopamine transporter locus (DAT1) and DSM-III-R-diagnosed ADHD (N = 49) and undifferentiated attention-deficit disorder (UADD) (N = 8) in trios composed of father, mother, and affected offspring. HHRR analysis revealed ...

1995-04-01

218

Three novel mutations responsible for Cockayne syndrome group A

Mutations in the FGD1 gene have been shown to cause Aarskog-Scott syndrome (AAS), or facio-digito-genital dysplasia (OMIM#305400), an X-linked disorder characterized by distinctive genital and skeletal developmental abnormalities with a broad spectrum of clinical phenotypes. To date, 20 distinct mutations have been reported, but little phenotypic data are available on patients with molecularly confirmed AAS. In the present study, we report on our experience of screening for mutations in the FGD1 gene in a cohort of 60 European patients with a clinically suspected diagnosis of AAS. We identified nine novel mutations in 11 patients (detection rate of 18.33%), including three missense mutations (p.R402Q; p.S558W; p.K748E), four truncating mutations (p.Y530X; p.R656X; c.806delC; c.1620delC), one in-frame deletion (c.2020_2022delGAG) and the first reported splice site mutation (c.1935+3A>C). A recurrent mutation (p.R656X) was ...

2010-01-01

219

Genome analysis of F. nucleatum sub spp vincentii and its comparison with the genome of F. nucleatum ATCC 25586.

Cockayne syndrome (CS) is a rare autosomal recessive disease, which shows diverse clinical symptoms such as photosensitivity, severe mental retardation and developmental defects. CS cells are hypersensitive to killing by ultraviolet (UV)-irradiation and defective in transcription-coupled repair. Two genetic complementation groups in CS (CS-A and CS-B) have been identified. We analyzed mutations of the CSA gene in 5 CS-A patients and identified 3 types of mutations. Four unrelated CS-A patients (CS2OS, CS2AW, Nps2 and CS2SE) had a deletion including exon 4, suggesting that there is a founder effect on the CSA mutation in Japanese CS-A patients. Patient CS2SE was a compound heterozygote for this deletion and an amino acid substitution at the 106th glutamine to proline (Q106P) in the WD-40 repeat motif of the CSA protein, which resulted in a defective nucleotide excision repair. Patient Mps1 had a large deletion in the ...

2003-02-01

220

Genetic organization of Bungarus multicinctus protease inhibitor-like proteins.

We present the draft genome sequence and its analysis for Fusobacterium nucleatum sub spp. vincentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN). A total of 441 FNV open reading frames (ORFs) with no orthologs in FN have been identified. Of these, 118 ORFs have no known function and are unique to FNV, whereas 323 ORFs have functional orthologs in other organisms. In addition to the excretion of butyrate, H2S and ammonia-like FN, FNV has the additional capability to excrete lactate and aminobutyrate. Unlike FN, FNV is likely to incorporate galactopyranose, galacturonate, and sialic acid into its O-antigen. It appears to transport ferrous iron by an anaerobic ferrous transporter. Genes for eukaryotic type serine/threonine kinase and phosphatase, transpeptidase E-transglycosylase Pbp1A are found in FNV but not in FN. Unique ABC transporters, cryptic phages, and three types of restriction-modification systems have been ...

2003-06-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

Study of a loop heat pipe using neutron radiography.

The structural organization of the genes encoding Bungarus multicinctus protease inhibitor-like proteins (PILPs), PILP-1, PILP-2 and PILP-3, are reported in this study. Unlike PILP-2 and PILP-3, recombinant PILP-1 exhibited inhibitory activity on trypsin. PILP genes and B chain genes shared identical organization with three exons interrupted by two introns in similar positions. On the contrary, intron 1 of these genes had a similar size, a notable variation with the size of intron 2 was observed. It was found that two regions at the second intron of B1 chain and B2 chain genes were absent in that of PILP genes. Noticeably, intronic insertion in the second intron of B chain genes appeared in the promoter region of PILP-1 gene, but not in that of PILP-2 and PILP-3 genes. Comparative analyses of PILP ...

2008-03-27

222

Study of a loop heat pipe using neutron radiography

An explanation is given of what a loop heat pipe (LHP) is, and how it works. It is then shown that neutron imaging (both real time neutron radioscopy and single exposure neutron radiography) is an effective experimental tool for the study of LHPs. Specifically, neutron imaging has helped to identify and correct a cooling water distribution problem in the condenser, and has enabled visualization of two-phase flow (liquid and vapor) in various components of the LHP. In addition, partial wick dry-out, a phenomenon of great importance in the effective operation of LHPs, is potentially identifiable with neutron imaging. It is anticipated that neutron radioscopy and radiography will greatly contribute to our understanding of LHP operation, and will lead to improvement of LHP modeling and design. PMID:15246420

2004-10-01

223

Remedial investigation/feasibility study for the David Witherspoon, Inc., 901 Site, Knoxville, Tennessee: Volume 1

An explanation is given of what a loop heat pipe (LHP) is, and how it works. It is then shown that neutron imaging (both real time neutron radioscopy and single exposure neutron radiography) is an effective experimental tool for the study of LHPs. Specifically, neutron imaging has helped to identify and correct a cooling water distribution problem in the condenser, and has enabled visualization of two-phase flow (liquid and vapor) in various components of the LHP. In addition, partial wick dry-out, a phenomenon of great importance in the effective operation of LHPs, is potentially identifiable with neutron imaging. It is anticipated that neutron radioscopy and radiography will greatly contribute to our understanding of LHP operation, and will lead to improvement of LHP modeling and design.

2004-10-01

224

This remedial investigation (RI)/feasibility study (FS) supports the selection of remedial actions for the David Witherspoon, Inc. 901 Maryville Pike Site in Knoxville, Tennessee. Operations at the site, used as a recycling center, have resulted in past, present, and potential future releases of hazardous substances in to the environment. This Site is a Tennessee Superfund site. A phased approach was planned to (1) gather existing data from previous investigations managed by the Tenn. Dept. of Environment and Conservation; (2) perform a preliminary RI, including risk assessments, and an FS with existing data to identify areas where remedial action may be necessary; (3) gather additional field data to adequately define the nature and extent of risk-based contaminants that present identifiable threats to human and/or ecological receptors; and (4) develop remedial action alternatives to reduce risks to acceptable levels.

1996-10-01

225

Explosives detector

Application of chaos theory in identification of two-phase flow patterns and transitions in a small, horizontal, rectangular channel

An apparatus for non-invasively inspecting an object, such as an item of luggage, for explosives material is described. It comprises a multi-channel thermal neutron inspection system having a plurality of neutron irradiation chambers. Simultaneous operation of several channels increases the maximum system throughput several times. Each chamber has a lithium neutron source which is stimulated to neutron production by a proton beam. Beam switching magnets are energised by pulsing to divert a common proton beam to each source in turn. The initial beam is generated by a radio frequency quadrupole accelerator. The advantages of this system are very low residual source activity and controllable neutron production thereby minimising safety hazards. The irradiation chamber may contain several different gamma ray detectors to identify the elements present in explosives material. In addition, a neutron radiography imaging means may be employed to ...

1991-10-02

226

An analysis of tanker-based floating production systems for small offshore fields

Various measurement tools that are used in chaos theory were applied to analyze two-phase pressure signals with the objective of identifying and interpreting flow pattern transitions for two-phase flows in a small, horizontal rectangular channel. These measurement tools included power spectral density function, autocorrelation function, pseudo-phase-plane trajectory, Lyapunov exponent,s and fractal dimensions. It was demonstrated that the randomlike pressure fluctuations characteristic of two-phase flow in small rectangular channels are chaotic, and governed by a high-order deterministic system. The correlation dimension is potentially a new approach for identifying certain two-phase flow patterns and transitions.

1996-06-01

227

Activation of a multisensory, multifunctional nucleus in the zebrafish midbrain during diverse locomotor behaviors

A historical review of floating production storage and offloading (FPSO) systems for marginal fields is presented. The areas in which such systems are operating, ranges of model tests, and current applicability of mooring systems are reviewed to present capabilities of these concepts. Limitations are reviewed to assess the applicability of these concepts to similar sites. Consideration of processing operations on floating vessels are identified. Particularly, the effects of vessel motion on the processing equipment, gas disposal, and crude offloading are addressed. It will be shown that operations are becoming well understood and routine. Future problem areas and solutions under development are identified. Specific examples are cited and design refinements are noted. While various floating production systems have been in operation for only a few years, their potential for solving the problems of producing small offshore ...

1982-08-01

228

British Library Electronic Table of Contents (United Kingdom)

Action potentials from the brain control the activity of spinal neural networks to produce, by as yet unknown mechanisms, a variety of motor behaviors. Particularly lacking are details on how identified descending neurons integrate diverse sensory inputs to generate specific locomotor patterns. We have examined the operations of the principal neurons in an intriguing midbrain nucleus, the nucleus of the medial longitudinal fasciculus (nMLF), in the larval zebrafish. The nMLF is the most rostral grouping of neurons that projects from the brain well into the spinal cord of teleost fishes, yet there is little direct physiological data available regarding its function. We report here that a distinct set of large, individually-identifiable neurons in nMLF (the MeL and MeM neurons) are activated...

2010-01-01

229

A single-institution review of 157 patients presenting with benign and malignant tumors of the ampulla of Vater: Management and outcomes

British Library Electronic Table of Contents (United Kingdom)

BackgroundAlthough benign ampullary tumors are removed endoscopically, due to their potential to progress to malignant disease, the favored treatment for adenocarcinoma is pancreaticoduodenectomy. We reviewed our institutions experience in order to identify which patients were at highest risk of disease progression following surgical resection, as well as evaluate whether localized T1 tumors are best treated by pancreaticoduodenectomy. MethodsWe retrospectively reviewed 157 patients who presented with an ampullary mass, from 2001 to 2010, and identified 51 with benign adenoma and 106 with adenocarcinoma. ResultsPatients with malignant tumors most often presented with larger tumors and jaundice, which alone was predictive of survival (OR = 67). Forty-five percent of patients with pathologic...

2011-01-01

230

Translational bioinformatics and healthcare informatics: computational and ethical challenges.

Somatic mutations detected by immunofluorescence and flow cytometry

Exponentially growing biological and bioinformatics data sets present a challenge and an opportunity for researchers to contribute to the understanding of the genetic basis of phenotypes. Due to breakthroughs in microarray technology, it is possible to simultaneously monitor the expressions of thousands of genes, and it is imperative that researchers have access to the clinical data to understand the genetics and proteomics of the diseased tissue. This technology could be a landmark in personalized medicine, which will provide storage for clinical and genetic data in electronic health records (EHRs). In this paper, we explore the computational and ethical challenges that emanate from the intersection of bioinformatics and healthcare informatics research. We describe the current situation of the EHR and its capabilities to store clinical and genetic data and then discuss the Genetic Information Nondiscrimination Act. Finally, we posit that the synergy obtained from ...

2009-09-16

231

Molecular prospecting for cryptic species in Phyllodistomum lacustri (Platyhelminthes, Gorgoderidae)

We have at hand the tools for development of both a single amino acid substitution and a gene loss type assay for somatic cell mutations in human red cells. We are close to being able to assess the assays as possible means for screening the human population for individuals who may be a risk of acquiring abnormally high frequencies of mutant cells. The cause for such a high frequency of mutant cells could be a prior exposure of the individual to large amount of mutagen, or it could indicate a member of a vulnerable subpopulation that may have poor DNA repair systems. Either of these cases could well be an indication that the particular individual is at high risk that exposure to mutagens could lead to potential pathology. In the near future we expect to determine the reliability of the assays and to perform experiments to confirm the validity of flow cytometry in measuring real somatic mutagenic events that occur in vivo. (orig./AJ).

232

British Library Electronic Table of Contents (United Kingdom)

Rosas-Valdez, R., Choudhury, A. & Prez-Ponce de Len, G. (2011). Molecular prospecting for cryptic species in Phyllodistomum lacustri (Platyhelminthes, Gorgoderidae). -Zoologica Scripta, 40, 296-305. Partial sequences of the 28S ribosomal RNA and cytochrome c oxidase I (COI) genes were compared among populations of a widely distributed and morphologically uniform digenean species in North America, Phyllodistomum lacustri, a parasite characteristically associated with ictalurid catfishes. Specimens were collected from the urinary bladder of ictalurid hosts in six localities of North America, spanning most of the latitudinal range of this freshwater fish group. Sequences of other congeneric species, including a potentially close relative, P.-staffordi, were also obtained and used for comparis...

2011-01-01

233

Hypomethylation and induction of retinoic acid receptor beta 2 by concurrent action of adenosine analogues and natural compounds in breast cancer cells

British Library Electronic Table of Contents (United Kingdom)

DNA methylation is considered as a potential cause of aberrations in regulation of gene expression during carcinogenesis. Therefore, changes in DNA methylation patterns may be targets for chemoprevention. In the present study, we investigated effects of all-trans retinoic acid (ATRA), vitamin D3, and resveratrol alone and in combination with adenosine analogues: 2-chloro-2'-deoxyadenosine (2CdA) and 9-beta-d-arabinosyl-2-fluoroadenine (F-ara-A), on methylation and expression of retinoic acid receptor beta 2 (RARbeta2) in MCF-7 and MDA-MB-231 breast cancer cell lines. Alterations in methylation and expression levels after treatment of cells with the tested compounds were evaluated by methylation-sensitive restriction analysis (MSRA) and real-time PCR, respectively. RARbeta2 promoter in the ...

2010-01-01

234

Amphiphysin (Amph) maps to the proximal region of mouse chromosome 13

Review of the occupational hygiene implications of the manufacture and use of nanoparticles

Amphiphysin is a protein concentrated in neuronal synapses and peripherally associated with neurotransmitter vesicles. It is expressed in many neurons of the central and peripheral nervous systems, in the adrenal medulla, in the anterior and posterior pituitary, in cell lines of the endocrine pancreas, and in spermatocytes. Its subcellular localization and tissue distribution indicate a potential involvement in mechanisms of regulated exocytosis. A role in the dynamic organization of the membrane-associated cytoskeleton is suggested by structural homology to the products of two yeast genes, RVS161 and RVS167, whose mutation results in an abnormal actin distribution, disturbs budding morphology, and impairs cell entry into stationary phase. Limited stretches of sequence similarity, including an SH3 domain, are also shared with other actin-binding proteins. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome, a ...

1995-07-20

235

Environmental Research Database

SummaryNanoparticles are increasingly being used in industry. Their potential to cause adverse health effects is due to their very small particles size (less than 100nm). A review is to be carried out to explore a) the use/release of nanoparticles in industry, b) current practices to control exposure and c) the potential levels of workplace exposure. The report will contain a discussion of what is current best practice to control exposure to nanoparticles. It will also indicate where the gaps in kn [continued...]ObjectivesA written report that draws upon disparate sources of available information and uses competent occupational hygiene expertise to form expert opinion on the implications. Key measures will the demonstration reasoned arguments supported by data from both current deliberate manufacturing of nanoparticles and incident nanoparticle production. The report will include all viable occupational exposure routes.Description1. ...

2004-01-29

236

A framework for assessing relative risks associated with multiple stressors in Port Valdez, Alaska

Waste heat recovery systems in the sugar industry: An Indian perspective

The purpose of this assessment is to develop a versatile process that will provide a mechanism for evaluating both present and future risks to this environment. Much of the regulatory and environmental interest in the port has centered around a Ballast Water Treatment facility that treats and discharges up to 30 mgd of oily ballast water brought in by crude oil tankers. However, six point discharges and other potential sources of pollution exist in the area. The authors have delineated eleven subareas in the port in order to identify the potential anthropogenic stressors, as well as the receptors that could be exposed to these stressors. Potential effects were then characterized for each exposure. Each component is ranked and integrated, resulting in a relative risk estimate in each subarea. Both the discernible risks, based on available data, and the data gaps are presented. Uncertainty is expressed as ...

1995-12-31

237

Synergies and conflicts in multimedia pollution control related to utility compliance with Title IV of the Clean Air Act Amendments of 1990

This article identifies the key role of the sugar industry in the rural development of developing countries. The Indian sugar industry, already second largest among the country`s processing industries, shows even greater potential, according to the Plan Documents (shown in a table). The potential of waste heat in sugar processing plants, which produce white crystal sugar using the double sulphitation clarification process, is estimated at 5757.9 KJ/kg of sugar. Efficient waste heat recovery (WHR) systems could help arrest the trend of increasing production costs. This would help the sugar industry not only in India, but in many other countries as well. The innovative methods suggested and discussed briefly in this article include dehydration of prepared cane, bagasse drying, and juice heating using waste heat. These methods can reduce the cost of energy in sugar production by at least 10% and improve efficiency and ...

1996-04-01

238

Rocky Mountain Arsenal, Basin F liquid storage tank spill, draft risk assessment

Most analyses of the alternative strategies used by utilities to comply with Title IV requirements have focused on factors directly related to controlling sulfur dioxide (SO{sub 2}) emissions. However, utilities must meet a number of additional environmental requirements at the same tune they comply with the acid rain program. To illuminate the potential synergies and conflicts that might exist between the other regulatory mandates and the acid rain program, a thorough examination of all the various programs and their interrelationships must be conducted. This paper reviews the environmental mandates that utilities will have to plan to meet in the next decade concurrently with the acid rain program, and it analyzes the interactions among the various programs to identify potential synergies and conflicts.

1993-11-01

239

Hot Cell Facility (HCF) Safety Analysis Report

The scope of this RA is limited to the evaluation of potential human health risks associated with a failure event of a tank containing Basin F liquid. Section 1.0 contains an introduction to the health risk assessment. Section 2.0 describes the site history, location, and land use. Section 3.0 provides a brief description of exposure pathways and potention receptors. Section 4.0 describes the sources of data used and identifies chemicals of concern. Section 5.0 discusses the toxicity of concern. Section 6.0 explains the methods used for calculation of carcinogenic risks and the noncarcinogenic hazard indexes. Section 7.0 describes uncertainties inherent in the current methodology used to determine potential human health risks. Section 8.0 presents a summary of results and conclusions. Section 9.0 includes the references cited.

1993-04-01

240

Energy conservation potential in Taiwanese textile industry

This Safety Analysis Report (SAR) is prepared in compliance with the requirements of DOE Order 5480.23, Nuclear Safety Analysis Reports, and has been written to the format and content guide of DOE-STD-3009-94 Preparation Guide for U. S. Department of Energy Nonreactor Nuclear Safety Analysis Reports. The Hot Cell Facility is a Hazard Category 2 nonreactor nuclear facility, and is operated by Sandia National Laboratories for the Department of Energy. This SAR provides a description of the HCF and its operations, an assessment of the hazards and potential accidents which may occur in the facility. The potential consequences and likelihood of these accidents are analyzed and described. Using the process and criteria described in DOE-STD-3009-94, safety-related structures, systems and components are identified, and the important safety functions of each SSC are described. Additionally, information which describes the safety ...

2000-11-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Dual-fuel natural gas/diesel engines: Technology, performance, and emissions. Topical report, February 1993-November 1994

Since Taiwan lacks sufficient self-produced energy, increasing energy efficiency and energy savings are essential aspects of Taiwan's energy policy. This work summarizes the energy savings implemented by 303 firms in Taiwan's textile industry from the on-line Energy Declaration System in 2008. It was found that the total implemented energy savings amounted to 46,074 ton of oil equivalent (TOE). The energy saving was equivalent to 94,614 MWh of electricity, 23,686 kl of fuel oil and 4887 ton of fuel coal. It represented a potential reduction of 143,669 ton in carbon dioxide emissions, equivalent to the annual carbon dioxide absorption capacity of a 3848 ha plantation forest. This study summarizes energy-saving measures for energy users and identifies the areas for making energy saving to provide an energy efficiency baseline.

2010-11-01

242

Assessing the environmental impacts of resource recovery facilities

An investigation of current dual-fuel natural gas/diesel engine design, performance, and emissions was conducted. The most pressing technological problems associated with dual-fuel engine use were identified along with potential solutions. It was concluded that dual-fuel engines can achieve low NOx and particulate emissions while retaining fuel-efficiency and BMEP levels comparable to those of diesel engines. The investigation also examined the potential economic impact of dual-fuel engines in diesel-electric locomotives, marine vessels, farm equipment, construction, mining, and industrial equipment, and stand-alone electricity generation systems. Recommendations for further additional funding to support research, development, and demonstration in these applications were then presented.

1994-11-01

243

Assessing Ground Water Development Potential Using Landsat Imagery

Technologies and practices that recover energy and materials from municipal solid waste present a technically and economically viable alternative to sanitary landfills and other solid waste disposal methods. However, community planners considering resource recovery should be aware of the potential environmental impacts of proposed projects. To assess these impacts, a planner should: identify the most significant types of impacts; establish boundaries beyond which impacts are not significant; characterize the environment as it would exist without the proposed project over the project's lifetime; predict the magnitude, and the distribution over time and space, of potential impacts; and relate the magnitude of predicted impacts to a scale of significance that will allow environmental values to be considered in decision-making. 24 references, 1 table.

1980-03-01

244

British Library Electronic Table of Contents (United Kingdom)

Seven villages in southeastern Kenya surround Mt. Kasigau and depend on the mountain's cloud forest for their water supply. Five of these villages have regularly experienced water shortages, and all village water supplies were contaminated with Escherichia coli bacteria. There is a need to economically find new sources of fresh ground water. Remote sensing offers a relatively quick and cost-effective way of identifying areas with high potential for ground water development. This study used spectral properties of features on Landsat remote sensing imagery to map linear features, soil types, surface moisture, and vegetation. Linear features represented geologic or geomorphologic features indicating either shallow ground water or areas of increased subsurface hydraulic conductivity. Regarding...

2010-01-01

245

Transcription regulation of the vegf gene by the BMP/Smad pathway in the angioblast of zebrafish embryos

Sida rhomboidea. Roxb Leaf Extract Down-Regulates Expression of PPAR?2 and Leptin Genes in High Fat Diet Fed C57BL/6J Mice and Retards in Vitro 3T3L1 Pre-Adipocyte Differentiation.

Vascular endothelial growth factor (VEGF) is a mitogen that is critically involved in vasculogenesis, angiogenesis, and hematopoiesis. However, what and how transcription factors participate in the regulation of vegf gene expression are not fully understood. Here we report the cloning and sequencing of the zebrafish vegf promoter which revealed that the promoter contains a number of bone morphogenetic protein (BMP)-activated Smad binding elements (SBE), implicating Smad1 and Smad5 in the regulation of BMP-induced expression of vegf. Electrophoretic mobility shift assays of adding recombinant Smad proteins to the SBE-containing DNA oligonucleotides that represent portions of zebrafish vegf promoter resulted in mobility shift of the oligonucleotides. These changes demonstrate potential interactions between Smad1/5 and the vegf promoter. Reporter activity assays using the wild-type or SBE-deleted vegf promoters to drive the luciferase reporter ...

2005-04-01

246

Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-B1

Sida rhomboidea. Roxb leaf extract (SRLE) is being used by the populace of North-East India to alleviate symptoms of diabetes and obesity. We have previously reported its hypolipidemic and anti-diabetic properties. In this study, we report the effect of SRLE on (i) in vivo modulation of genes controlling high fat diet (HFD) induced obesity and (ii) in vitro 3T3L1 pre-adipocyte differentiation and leptin release. Supplementation with SRLE significantly prevented HFD induced increment in bodyweight, plasma lipids and leptin, visceral adiposity and adipocyte hypertrophy. Also, SRLE supplementation reduced food intake, down regulated PPAR?2, SREBP1c, FAS and LEP expressions and up-regulated CPT-1 in epididymal adipose tissue compared to obese mice. In vitro adipogenesis of 3T3L1 pre-adipocytes was significantly retarded in the presence of SRLE extract. Also decreased triglyceride accumulation, leptin release and glyceraldehyde-3-Phosphate dehydrogenase activity along ...

2011-07-19

247

Development of recombinant adeno-associated virus and adenovirus cocktail system for efficient hTERTC27 polypeptide-mediated cancer gene therapy.

Mesenchymal stem cells (MSCs) are a potential source of smooth muscle cells (SMCs) for constructing tissue-engineered vascular grafts. However, the details of how specific combinations of vascular microenvironmental factors regulate MSCs are not well understood. Previous studies have suggested that both mechanical stimulation with uniaxial cyclic strain and chemical stimulation with transforming growth factor {beta}1 (TGF-{beta}1) can induce smooth muscle markers in MSCs. In this study, we investigated the combined effects of uniaxial cyclic strain and TGF-{beta}1 stimulation on MSCs. By using a proteomic analysis, we found differential regulation of several proteins and genes, such as the up-regulation of TGF-{beta}1-induced protein ig-h3 (BGH3) protein levels by TGF-{beta}1 and up-regulation of calponin 3 protein level by cyclic strain. At the gene expression level, BGH3 was induced by TGF-{beta}1, but calponin 3 was not ...

2009-10-12

248

Selection of reference soils for chemicals testing in the European Community

The low in vivo transduction efficiency of recombinant adeno-associated virus (rAAV) and the undesirably strong immunogenicity of adenovirus (rAdv) have limited their clinical utilization in cancer gene therapy. We have previously demonstrated that intratumoral injection of rAAV expressing a C-terminal polypeptide of human telomerase reverse transcriptase (rAAV-hTERTC27) effectively inhibits the growth of glioblastoma xenografts in nude mice. To further improve its efficacy, we combined rAAV-hTERTC27 with rAdv and investigated the efficiency of the cocktail vectors in vivo. At a nontherapeutic dose (1 x 10(8) plaque-forming units (PFUs)), rAdv-null and rAdv-hTERTC27 were equipotent in enhancing the therapeutic efficacy of rAAV-hTERTC27 (1.5 x 10(11) v.g.), and complete tumor regression was achieved in 25% of the treated animals. Importantly, the combination of rAAV-hTERTC27 and a therapeutic dose (2.5 x 10(9) PFU) of rAdv-hTERTC27 significantly augmented the ...

2008-06-06

249

Natural fracture characterization using passive seismic illumination

Based on an multivariate statistical evaluation of binary and metric data relating to the soil cover of the European Community five regionally representative reference soils (EURO-Soils) have been identified for chemicals testing in the EC. The soil material sampled at representative localities in Italy, Greece, Great Britain, France and Germany was treated and prepared according to OECD Test Guideline 106 and analysed in detail. The homogenised specimens were subject to an EC-wide ring test to evaluate the feasibility of the modified guideline and to validate the physical-chemical amenability of the reference soils for sorption tests. The results proved the validity of the soils selected for assessing the potential behaviour of new chemicals in soil on the basis of a comparative evaluation of the individual test results obtained. In the light of this parametric assessment potential test soils were subsequently ...

250

Identification of environmental RD D needs

The presence of natural fractures in reservoir rock can significantly enhance gas production, especially in tight gas formations. Any general knowledge of the existence, location, orientation, spatial density, and connectivity of natural fractures, as well as general reservoir structure, that can be obtained prior to active seismic acquisition and drilling can be exploited to identify key areas for subsequent higher resolution active seismic imaging. Current practices for estimating fracture properties before the acquisition of surface seismic data are usually based on the assumed geology and tectonics of the region, and empirical or fracture mechanics-based relationships between stratigraphic curvature and fracturing. The objective of this research is to investigate the potential of multicomponent surface sensor arrays, and passive seismic sources in the form of local earthquakes to identify and characterize ...

2003-01-08

251

Hazard analysis for 300 Area N Reactor Fuel Fabrication and Storage Facilty

The objective of this project was to identify needs for environmental research development and demonstration projects that have the potential to improve the performance of oil and gas exploration, drilling, and production technologies for maximum recovery of domestic petroleum resources under optimal environmental and economic conditions. To achieve this objective several areas of work were addressed. The first task was to compile as much related data as possible. This was achieved by literature searches of a number of petroleum-related data bases. After acquiring sufficient background on environmental and economical areas that should be expanded, experts who are knowledgeable in these areas were contacted to further define specific issues. More than 33 identified areas were submitted to an in-house panel at NIPER to assure that the final environmental research demonstration and development needs that were selected to be ...

1991-09-01

252

Development of advanced residential cooktop burner with low NO/sub x/ emissions. Annual report Feb 82-Jan 83

This hazard analysis (HA) has been prepared for the 300 Area N Reactor Fuel Fabrication and Storage Facility (Facility), in compliance with the requirements of Westinghouse Hanford Company (Westinghouse Hanford) controlled manual WHC-CM-4-46, Nonreactor Facility Safety Analysis Manual, and to the direction of WHC-IP-0690, Safety Analysis and Regulation Desk Instructions, (WHC 1992). An HA identifies potentially hazardous conditions in a facility and the associated potential accident scenarios. Unlike the Facility hazard classification documented in WHC-SD-NR-HC-004, Hazard Classification for 300 Area N Reactor Fuel Fabrication and Storage Facility, (Huang 1993), which is based on unmitigated consequences, credit is taken in an HA for administrative controls or engineered safety features planned or in place. The HA is the foundation for the accident analysis. The significant event scenarios identified by ...

1994-01-25

253

Avoidable thermodynamic inefficiencies and costs in an externally fired combined cycle power plant

This report describes the results of work conducted during February 1982 - January 1983. A literature search was initiated to identify the state-of-the-art of NOx (NO + NO/sub 2/) reduction technologies, including NOx measurement methodologies, air infiltration and ventilation trends, the impact of NOx (NO/sub 2/) emissions on health, and air quality standards. As a result of the literature search NOx (NO/sub 2/) reduction goals were established for the project and technologies to achieve these goals identified. This report also covers the development of a prototype burner which examined flue gas recirculation, burner designs, secondary air baffling and flame inserts as potential technologies to reduce NOx (NO/sub 2/). Because of their potential for NOx (NO/sub 2/) reduction shown by initial experiments, an in-depth analysis of ring insert configurations was conducted. These results, along with other ...

1983-02-28

254

Analysis of options for coal combustion waste management in the Pacific Basin

The real thermodynamic inefficiencies in a thermal system are related to exergy destruction and exergy loss. An exergy analysis identifies the system components with the highest exergy destruction and the processes that cause them. However, only a part of the exergy destruction in a component can be avoided. A minimum exergy destruction rate for each system component is imposed by physical, technological, and economic constraints. The difference between the total and the unavoidable exergy destruction rate represents the avoidable exergy destruction rate, which provides a realistic measure of the potential for improving the thermodynamic efficiency of a component. The calculation of avoidable cost rates associated with both exergy destruction and capital investment is described in the paper and is applied to the exergoeconomic evaluation of an externally fired combined cycle power plant. For each plant component, avoidable and unavoidable ...

2006-08-15

255

Virtual colonoscopy (CT colonography) in the identification of colorectal cancer. A prospective study in symptomatic patients

Many Pacific Basin countries rely on oil for electricity production. Alternative fuel sources such as coal, which is available in the Pacific Basin, can help mitigate adverse impacts of sudden price increases or supply disruptions. Coal combustion produces solid and potentially hazardous wastes of concern to environmental regulators and utility managers. This paper identifies issues associated with managing coal combustion wastes in the Pacific Basin, using the state of Hawaii as a case study. Hawaii is typical of many Pacific Basin locations in that it depends on oil, has limited sites, for waste management operations, and is subject to domestic and international waste management regulations. The paper discusses coal-fired utility wastes, environmental impacts of coal combustion waste disposal, and regulatory requirements that impact coal waste management. From this baseline, potential on- and off-island options for coal ...

1993-10-01

256

Overview of the environmental concerns of coal transportation

Aim of this study was to evaluate the sensitivity of virtual colonoscopy (CT colonography) in the identification of colorectal cancer and to define the limitations and the advantages of this imaging modality, as well as indications to the examination. It was examined prospectively 62 symptomatic patients aged 36 to 82 years (28 women and 34 men). All patients underwent both conventional and virtual colonoscopy on the same day; the conventional examination allowed exploration of the entire colon. Conventional colonoscopy identified 89 lesions 3-50 mm in diameter, namely 84 benign and 5 malignant lesions. No lesions were identified in 12 patients. CT colonography identified 52 of the 89 lesions, with 57.1% diagnostic accuracy. They were 11 false positives (82.5% positive predictive value and 52.2% specificity) and 37 false negatives (24.5% negative predictive value and 58.4% sensitivity). Sensitivity was significantly higher ...

2000-06-01

257

Assessing digital control system dependability using the dynamic flowgraph methodology

More than 30 environmental concerns were analyzed for the transportation of coal by rail, roads (trucks), high voltage transmission lines (that is, from mine-mouth generating plants to distribution networks), coal slurry pipelines, and barges. The following criteria were used to identify these problems: (1) real physical environmetal impacts for which control technologies must be developed, or regulation made effective where control technologies presently exist; (2) the level of impact is uncertain, although the potential impact may be moderate to high; (3) the concerns identified by the first two criteria are specific to or exacerbated by coal transportation. Generic transportation problems are not included. The significant environmental problems identified as a result of this study are: (1) rail transport - community traffic disruption and human health, safety, and habitat destruction; (2) coal haul ...

1980-02-01

258

Americans' political participation in the 1993-94 national health care reform debate.

Dynamic Flowgraph Methodology (DFM) is a methodological approach to modeling and analyzing the behavior of software-driven embedded systems for the purpose of reliability/safety assessment and verification. The methodology has two fundamental goals: (a) to identify how certain postulated events may occur in a system and (b) to identify an appropriate testing strategy based on an analysis of system functional behavior. To achieve these goals, the methodology employs a modeling framework in which system models are developed in terms of causal relationships between physical variables and temporal characteristics of the execution of software modules. These models are then analyzed to determine how a certain state (desirable or undesirable) can be reached. This is done by developing timed fault trees, which take the form of logical combinations of static trees relating system parameters at different points in time. The prime implicants (multistate ...

1993-01-01

259

Towards Antihydrogen Trapping and Spectroscopy at ALPHA

The health politics and policy communities are still struggling with the question of "what went wrong" in the 1993-94 health care reform effort. Here I identify which Americans were politically active and inactive during the health care reform debate to explore the role political participation may have had in determining the outcome of the debate. Using data from a national and California random-sample telephone surveys, and controlling for other demographic attributes, I found that those who engaged in political activity specifically related to health care reform were disproportionately more likely to be self-identified conservatives, less likely to favor an employer mandate plan, more likely to be fifty to sixty-four years old, more likely to be men, and more likely to have greater interest in and knowledge of the health care issue. Even in California, where a single-payer proposal was on the November ballot, ...

1996-01-01

260

CERN Document Server

Spectroscopy of antihydrogen has the potential to yield high-precision tests of the CPT theorem and shed light on the matter-antimatter imbalance in the Universe. The ALPHA antihydrogen trap at CERN's Antiproton Decelerator aims to prepare a sample of antihydrogen atoms confined in an octupole-based Ioffe trap and to measure the frequency of several atomic transitions. We describe our techniques to directly measure the antiproton temperature and a new technique to cool them to below 10 K. We also show how our unique position-sensitive annihilation detector provides us with a highly sensitive method of identifying antiproton annihilations and effectively rejecting the cosmic-ray background.

2011-01-01

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261

Potential vulnerabilities of nuclear fuel cycle facilities to the year 2000 (Y2K) issue and measures to address them

PPAR action in insulin resistance unraveled by metabolomics: potential clinical implications.

The exchange of information and experience among Member Sates is an essential component of the IAEA action plan for addressing the Year 2000 problem. The objective is to enable Member States to identify any gaps in their own conversion programmes, benefit form the experience of others in developing remedial actions and establish the basis for future action to solve remaining problems. Experts in Year 2000 issues particularly those related to digital equipment prepared this report dealing with nuclear fuel cycle facilities

1993-04-18

262

Modelling and measurement of radon diffusion through soil for application on mine tailings dams

ABSTRACT: Metabolomic analysis will provide the next large set of clues to further our understanding of human health and disease. A recent study has elucidated the significant differences in the metabolomes of adipocytes, serum and an adipocyte cell line after activation of two nuclear receptors, peroxisome proliferator activated receptor ?/? (PPAR?/?) and PPAR?. These findings hold great promise for explaining fundamental differences in the mechanisms of PPAR agonists and for identifying targets for the treatment of diabetes.See related research article: http://genomebiology.com/2011/12/8/R75. PMID:21861850

2011-08-23

263

Managing risks and hazardous in industrial operations

The mine dumps that arise from the gold mining operations in South Africa are a potential source of high concentrations of radon (222"Rn). Studying the diffusion of radon and the emanation from the soil will help to deduce the radon flux from these dumps to identify the problem areas for rehabilitation. This study describes measurements of the emanation coefficient and the modelling of the depth profile of the radon activity concentration, which is compared to a depth profile that was measured on such a mine dump. Emanation coefficients ranging from 0.13 to 0.39 have been obtained.

2005-04-01

264

In Situ Remediation of {sup 137}Cs Contaminated Wetlands Using Naturally Occurring Minerals

The main objective of this paper is to demonstrate that it makes good business sense to identify risks and hazards of an operation and take appropriate steps to manage them effectively. Developing and implementing an effective risk and hazard management plan also contibutes to other industry requirements and standards. Development of a risk management system, key elements of a risk management plan, and hazards and risk analysis methods are outlined. Comparing potential risk to the cost of prevention is also discussed. It is estimated that the cost of developing and preparing the first risk management plan varies between $50,000 to $200,000. 3 refs., 2 figs., 1 tab.

1996-12-31

265

Go vs. no-go - potential and limitations of continuous-variable quantum computing by measurements

Cesium-137 has contaminated a large area of the wetlands on the Savannah River Site. Remediation of the contaminated wetlands is problematic because current techniques destroy the sensitive ecosystem and generate a higher dose to workers. To address this problem, we proposed a non-trusive, in situ technology to sequester 137Cs in sediments. One intention of this study was to provide information regarding a go/no go decision for future work. Since the proof-of-concept was successful and several minerals were identified as potential candidates for this technology, a go decision was made.

1999-08-11

266

Geothermal resource assessment of the New England states

In this talk, we explore the feasibility of quantum computation using continuous-variable systems by means of local measurements only. In the first part of the talk, we will identify crucial limitations that arise when starting from Gaussian cluster states. This is done by resorting to a Gaussian projected entangled pair picture as well as to notions of continuous-variable quantum repeater networks. In the second part, we look at instances in which these limitations can be overcome, and how suitable encodings of qubits in oscillators and feasible non-Gaussian resource states give rise to universal schemes for quantum computing.

2010-07-01

267

Faster-than-light particles: a review of tachyon characteristics. Interim report

With the exception of Sand Springs in Williamstown, Massachusetts, there are no identifiable hydrothermal geothermal resources in the New England region. The radioactive plutons of the White Mountains of New Hampshire do not, apparently, contain sufficient stored heat to make them a feasible target for an induced hydrothermal system such as exists at Fenton Hill near Los Alamos, New Mexico. The only potential source of low grade heat is the large volume of ground water contained within the unconsolidated sediments related to the Pleistocene glaciation of the region. During the course of the survey an unusual and unexplained thermal anomaly was discovered in St. Johnsbury, Vermont, which is described.

1982-01-01

268

Electron combinational light scattering in disordered Ti_x_-_1Fe_x alloys: theory and experiment

This report documents an analytical prediction of some of the characteristics which presently undiscovered faster-than-light sub-atomic particles (called tachyons) must possess if they are to exist without violating the Theory of Special Relativity. A brief review of necessary concepts from the Special Theory is included so that the reader might more readily understand the reasoning as it is developed. Necessary, but not all, characteristics of tachyons are then identified and presented. Finally, an interesting potential relationship between tachyons and anti-gravity is discussed.

1980-10-01

269

Compaction of salt by means of explosives

Theoretical description of Raman scattering in disordered alloys of Ti and Zr with transition metals of groups 5-8 of the Mendeleev Periodic System in critical range of concentrations (CRC) is given. Results of Raman scattering experiment in the system Ti_1_-_xF_x at 0.0005identified by previously made Moessbauer measurements for the same samples, are presented. The order of the values of hybridization potential and energy of impurity zone, as well as their concentrational dynamics, are determined. Qualitative adequacy of the hybridization model to the alloys studied near CRC is shown.

270

A quantitative method to detect explosives and selected semivolatiles in soil samples by Fourier transform infrared spectroscopy

One of the concerns with locating radioactive waste storage sites in salt deposits is how to permanently seal the underground storage areas once they have reached their storage capacity. The compaction of salt using explosives has been identified as a potential method of producing permanent seals in both entryways and shafts to storage areas. This paper describes the test procedure and results of a preliminary investigation to determine the feasibility of utilizing explosives in the compaction of salt. Three simple tests were carried out to measure the degree to which loose salt could be compacted.

1996-12-01

271

Safe ageing management of nuclear power plants: An European synthesis

This paper describes a novel Fourier transform infrared (FTIR) spectroscopic method that can be used to rapidly screen soil samples from potentially hazardous waste sites. Samples are heated in a thermal desorption unit and the resultant vapors are collected and analyzed in a long-path gas cell mounted in a FTIR. Laboratory analysis of a soil sample by FTIR takes approximately 10 minutes. This method has been developed to identify and quantify microgram concentrations of explosives in soil samples and is directly applicable to the detection of selected volatile organics, semivolatile organics, and pesticides.

1995-06-01

272

Sequence analysis of the ATM gene in 20 patients with RTOG grade 3 or 4 acute and/or late tissue radiation side effects

Ageing of nuclear power plants means evolution of material or equipment properties on one side, and evolution of personnel skill and procedure adequacy on the other side, both of which, after a certain time, may not be compatible with the required safety provisions, or with an economic operation of the plant. Repair or replacement of components, as well as change in service conditions for a better compatibility with component reduced capabilities can be used to mitigate ageing effects. The paper summarises the results of a study conducted in this field with the support of the European Commission. It presents: the synthesis of the work done under international auspices, and in the European context; the comparison of ageing management approaches used in several European countries with international recommendations; the summary of the various potential phenomena and their governing parameters, the methods of in-service ageing identification and possible mitigation ...

2002-11-04

273

piggyBac Transposon-mediated Long-term Gene Expression in Mice

Purpose: Patients with ataxia-telangiectasia (A-T) show greatly increased radiation sensitivity and cancer predisposition. Family studies imply that the otherwise clinically silent heterozygotes of this autosomal recessive disease run a 3.5 to 3.8 higher risk of developing cancer. In vitro studies suggest moderately increased cellular radiation sensitivity of A-T carriers. They may also show elevated clinical radiosensitivity. We retrospectively examined patients who presented with severe adverse reactions during or after standard radiation treatment for mutations in the gene responsible for A-T, ATM, considering a potential means of future identification of radiosensitive individuals prospectively to adjust dosage schedules. Material and Methods: We selected 20 cancer patients (breast, 11; rectum, 2; ENT, 2; bladder, 1; prostate, 1; anus, 1; astrocytoma, 1; Hodgkins lymphoma, 1) with Grade 3 to 4 (RTOG) acute and/or late tissue radiation side ...

1999-07-15

274

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection...Full Text Available

2010-04-01

275

The rapid generation of chimerical genes expanding protein diversity in zebrafish

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

276

The luxS Gene of Streptococcus pyogenes Regulates Expression of Genes That Affect Internalization by Epithelial Cells

BackgroundVariation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin...Full Text Available

277

The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination

The gram-positive pathogen Streptococcus pyogenes was recently reported to possess a homologue of the luxS gene that is responsible for the production of autoinducer...Full Text Available

2003-10-01

278

Specific genetic modifications of domestic animals by gene targeting and animal cloning

Activation-induced deaminase (AID) initiates somatic hypermutation, gene conversion and class switch recombination by deaminating variable and switch region DNA cytidines to uridines. AID is predominantly...Full Text Available

279

Reverse Engineering of Gene Regulatory Networks: A Comparative Study

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available

280

Recurrent miscarriage and variant alleles of mannose binding lectin, tumour necrosis factor and lymphotoxin ? genes

Reverse engineering of gene regulatory networks has been an intensively studied topic in bioinformatics since it constitutes an intermediate step from explorative to causative gene expression...Full Text Available

2009-01-01

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281

Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5_Hc Gene by Allelic Replacement

Variant alleles of the mannose binding lectin (MBL) gene are associated with increased susceptibility to infection and polymorphisms of tumour necrosis factor and lymphotoxin alpha genes (TNF, LTA)...Full Text Available

2001-12-01

282

Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum.

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5...Full Text Available

1998-10-01

283

Prediction of breast cancer prognosis using gene set statistics provides signature stability and biological context

We have examined the expression and structure of several genes belonging to two classes of vegetative specific genes of the simple eukaryote, Dictyostelium discoideum. In amebae grown on bacteria, deactivation...Full Text Available

1989-12-11

284

PET imaging of heat-inducible suicide gene expression in mice bearing head and neck squamous cell carcinoma xenografts

BackgroundDifferent microarray studies have compiled gene lists for predicting outcomes of a range of treatments and diseases. These have produced gene lists that have little overlap,...Full Text Available

285

Neuronatin: A New Inflammation Gene Expressed on the Aortic Endothelium of Diabetic Mice

The ability to achieve tumor selective expression of therapeutic genes is an area that needs improvement for cancer gene therapy to be successful. One approach to address this is through the...Full Text Available

2009-02-01

286

Mutational analysis of bacteriophage lambda lysis gene S.

OBJECTIVE—Identification of arterial genes and pathways altered in obesity and diabetes.RESEARCH DESIGN AND METHODS—Aortic gene expression profiles of...Full Text Available

2008-10-01

287

GeneSrF and varSelRF: a web-based tool and R package for gene selection and classification using random forest

A plasmid carrying the bacteriophage lambda lysis genes under lac control was subjected to hydroxylamine mutagenesis, and mutations eliminating the host lethality of the S gene were selected. DNA sequence...Full Text Available

1986-09-01

288

Gene-Environment Interactions and Epigenetic Basis of Human Diseases

BackgroundMicroarray data are often used for patient classification and gene selection. An appropriate tool for end users and biomedical researchers should combine user friendliness...Full Text Available

289

Gene expression analysis of interferon ? in laser capture microdissected cervical epithelium

Most human diseases are related in some way to the loss or gain in gene functions. Regulation of gene expression is a complex process. In addition to genetic mechanisms, epigenetic causes are...Full Text Available

2008-01-01

290

Evolution of DMY, a newly emergent male sex-determination gene of medaka fish.

Optimal sample handling techniques for tissue preparation and storage, RNA extraction and quantification, and target gene detection are crucial for reliable gene expression analysis. Methods...Full Text Available

2008-10-01

291

EDISA: extracting biclusters from multiple time-series of gene expression profiles

The Japanese medaka fish Oryzias latipes has an XX/XY sex-determination system. The Y-linked sex-determination gene DMY is a duplicate of the autosomal gene DMRT1, which encodes a DM-domain-containing...Full Text Available

2004-04-01

292

Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae.

BackgroundCells dynamically adapt their gene expression patterns in response to various stimuli. This response is orchestrated into a number of gene expression modules consisting...Full Text Available

293

Antisense Expression of the CK2 ?-Subunit Gene in Arabidopsis. Effects on Light-Regulated Gene Expression and Plant Growth1

We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen...Full Text Available

1992-11-11

294

Analysis of the bmp Gene Family in Borrelia burgdorferi Sensu Lato

The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because...Full Text Available

1999-03-01

295

An Efficient Fungal RNA-Silencing System Using the DsRed Reporter Gene?

BmpA, BmpB, BmpC, and BmpD are homologous Borrelia burgdorferi lipoproteins of unknown functions, encoded by the bmp genes of paralogous chromosomal gene family 36....Full Text Available

2000-04-01

296

Transcription Factor IIA t Is Associated with Undifferentiated Cells and Its Gene Expression Is Repressed in Primary Neurons at the Chromatin Level In Vivo

In filamentous fungi, RNA silencing is an attractive alternative to disruption experiments for the functional analysis of genes. We adapted the gene encoding the autofluorescent DsRed protein as a reporter...Full Text Available

2007-02-01

297

British Library Electronic Table of Contents (United Kingdom)

The levels of General Transcription Factor (TF) IIA were examined during mammalian brain development and in rat embryo fibroblasts and transformed cell lines. The large TFIIA subunit paralogues ab and t are largely produced in unsynchronized cell lines, yet only TFIIA ab is observed in a number of differentiated tissue extracts. Steady-state protein levels of the TFIIA t, ab, and g subunits were significantly reduced when human embryonal (ec) and hepatic carcinoma cell lines were stimulated to differentiate with either all-trans-retinoic acid (ATRA) or sodium butyrate. ATRA-treated NT2-ec cells required replating to induce a neuronal phenotype and loss of detectable TFIIA t and g proteins. High levels of TFIIA t, ab, and g and Sp factors were identified in extracts from human fetal and rat...

2006-01-01

298

Mechanisms of ozone tolerance in rice: characterization of two QTLs affecting leaf bronzing by gene expression profiling and biochemical analyses

British Library Electronic Table of Contents (United Kingdom)

High surface ozone concentration is increasingly being recognized as a factor that negatively affects crop yields in Asia. However, little progress has been made in developing ozone-tolerant genotypes of rice-Asias major staple crop. This study aimed to identify possible tolerance mechanisms by characterizing two quantitative trait loci (QTLs) that were previously shown to influence visible leaf symptoms under ozone exposure (120 nl l-1, 7 h d-1, 13 d). Two chromosome segment substitution lines (SL15 and SL41) that carried introgressions of the QTLs OzT3 and OzT9, respectively, were exposed to ozone at 120 nl l-1 along with their parent Nipponbare. In accordance with the expected QTL effect, SL15 showed stronger visible symptoms of ozone damage than Nipponbare, whereas SL41 had fewer sympt...

2010-01-01

299

Loss of PINK1 function decreases PP2A activity and promotes autophagy in dopaminergic cells and a murine model

British Library Electronic Table of Contents (United Kingdom)

Parkinson's disease (PD) is the most common neurodegenerative movement disorder. Mutations in PTEN-induced kinase 1 (PINK1) are a frequent cause of recessive PD. Autophagy, a pathway for clearance of protein aggregates or impaired organelles, is a newly identified mechanism for PD development. However, it is still unclear what molecules regulate autophagy in PINK1-silenced cells. Here we report that autophagosome formation is promoted in the early phase in response to PINK1 gene silencing by lentivirus transfer vectors expressed in mouse striatum. Reduced PP2A activity and increased phosphorylation of PP2A at Y307 (inactive form of PP2A) were observed in PINK1-knockdown dopaminergic cells and striatum tissues. Treatment with C2-ceramide (an agonist of PP2A) reduced autophagy levels in PINK...

2011-01-01

300

Identification, characterization, and chromosomal localization of the human homolog (hES) of ES/130

Ectopic expression of wild-type or a dominant-negative mutant of transcription factor NTF-1 disrupts normal Drosophila development.

The chicken extracellular matrix glycoprotein ES/130 is necessary for epithelial-mesenchymal transformation in the developing hear and is also expressed in noncardiac chicken tissues such as limb and notochord. We have identified hES, the human homology of chicken ES/130. Fluorescence in situ hybridization analysis (FISH) localizes hES to human chromosome 20p11.2-p12. FISH analyses of individuals with 20p12 deletions and affected by Alagille syndrome exclude hES as a candidate gene for this disorder. Reverse transcriptase-polymerase chain reaction studies reveal that hES is expressed in both fetal and adult human tissues and that hES expression in the left ventricle is increased in the failing adult heart. Further studies will evaluate how hES mutations may relate to congenital human cardiac and skeletal anomalies as well as cardiac remodeling in the adult. 16 refs., 2 figs.

1996-08-01

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301

The Drosophila melanogaster tissue-specific transcription factor NTF-1 was originally identified in vitro as a protein that could bind to and activate transcription from the Dopa decarboxylase (Ddc) gene. A structure-function analysis of NTF-1 led to the identification of a discrete amino-terminal activation domain. Here, we report that an NTF-1 mutant lacking the activation domain acts as a trans-dominant inhibitor of NTF-1 activation in tissue culture cells by forming inactive heterodimers with the full-length protein. Ectopically expressing this dominant-negative protein or the full-length protein in developing Drosophila embryos leads to dire developmental consequences. Overexpressing the trans-dominant NTF-1 leads to lethality, while overexpressing full-length NTF-1 results in both lethality and morphogenetic defects. Our results suggest that both the activity and the regulation of NTF-1 are critical for viability and proper development of ...

1993-11-15

302

Amyloidosis

A major QTL on chromosome 11 influences psychostimulant and opioid sensitivity in mice

The subjects covered in this Symposium range through almost every clinical medical specialty. From an average of one paper in each of the past three Symposiums, the explosive interest in cerebral amyloidosis has led to the presentation of 12 papers on this subject in the present volume. The genetically predisposed familial amyloidotic processes, such as the polyneuropathies and familial Mediterranean fever have also stimulated extensive and intriguing investigations which have revealed the striking effect of a single amino acid substitution in transforming a normal protein into a lethal ''amyloidogenic'' one. This Symposium clearly depicts the advances since the first amyloid fibril protein was definitively identified and defined 14 years ago. Since all amyloid fibril proteins so far described are variants of normal proteins, attention to gene abnormalities now becomes a significant focus as well as the pathogenic sequences which lead in these ...

1984-11-09

303

British Library Electronic Table of Contents (United Kingdom)

The identification of genes influencing sensitivity to stimulants and opioids is important for determining their mechanism of action and may provide fundamental insights into the genetics of drug abuse. We used a panel of C57BL/6J (B6; recipient)x A/J (donor) chromosome substitution strains (CSSs) to identify quantitative trait loci (QTL) for both open field activity and sensitivity to the locomotor stimulant response to methamphetamine (MA). Mice were injected with saline (days 1 and 2) and MA (day 3; 2 mg/kg i.p.). We analyzed the total distance traveled in the open field for 30 min following each injection. CSS-8, -11 and -16 showed reduced MA-induced locomotor activity relative to B6, whereas CSS-10 and -12 showed increased MA-induced locomotor activity. Further analysis focused on CSS...

2009-01-01

304

Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers

Occurrence of mutations in the epidermal growth factor receptor gene in X-ray-induced rat lung tumors

It is becoming clear that simple sequence repeats (SSRs) play a significant role in fungal genome organization, and they are a large source of genetic markers for population genetics and meiotic maps. We identified SSRs in the Laccaria bicolor genome by in silico survey and analyzed their distribution in the different genomic regions. We also compared the abundance and distribution of SSRs in L. bicolor with those of the following fungal genomes: Phanerochaete chrysosporium, Coprinopsis cinerea, Ustilago maydis, Cryptococcus neoformans, Aspergillus nidulans, Magnaporthe grisea, Neurospora crassa and Saccharomyces cerevisiae. Using the MISA computer program, we detected 277,062 SSRs in the L. bicolor genome representing 8% of the assembled genomic sequence. Among the analyzed basidiomycetes, L. bicolor exhibited the highest SSR density although no correlation between relative abundance and the genome sizes was observed. In most genomes the short motifs (mono- to ...

2011-01-01

305

IL-4/Stat6 activities correlate with apoptosis and metastasis in colon cancer cells

Epidermal growth factor receptor (EGFR) gene alterations have been found in human lung cancers. However, there is no information on the factors inducing EGFR mutations. In rodents, K-ras mutations are frequently found in many lung carcinogenesis models, but hitherto, Egfr mutations have not been reported. Their presence was therefore investigated in representative lung carcinogenesis models with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosobis(2-hydroxypropyl)amine (BHP), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MelQx) and ethyl carbamate (urethane), as well as X-ray irradiation. With the chemical carcinogenesis models, no mutations were detected in Egfr, which is in clear contrast to the high rates observed in either codon 12 or 61 of K-ras (21/23 of the lung tumors induced with NNK, 4/5 with MelQx, 1/4 with urethane and 7/18 with BHP). However, in the X-ray-induced lung tumors, Egfr mutations with amino acid substitution were observed in ...

2008-02-01

306

Cloning, chromosomal assignment, and regulation of the rat thyrotropin receptor: Expression of the gene is regulated by thyrotropin, agents that increase cAMP levels, and thyroid autoantibodies

IL-4-induced Stat6 signaling is active in a variety of cell types and plays a role in cell proliferation/growth and resistance to apoptosis. Using EMSA, we identified differential IL-4/Stat6 activities in colorectal cancer cell lines, HT-29 being active Stat6"h"i"g"h phenotype and Caco-2 being defective Stat6"n"u"l"l phenotype, respectively. Active Stat6"h"i"g"h HT-29 cells exhibited resistance to apoptosis by flowcytometry and aggressive metastasis by Transwell assay compared with defective Stat6"n"u"l"l Caco-2 cells. Comparing one another using RT-PCR, Stat6"h"i"g"h HT-29 cells expressed more mRNA of anti-apoptotic and pro-metastatic genes Survivin, MDM2, and TMPRSS4, while Stat6"n"u"l"l Caco-2 cells expressed more mRNA of pro-apoptotic and anti-metastatic genes BAX, CAV1, and P53, respectively. This is the first study describing correlations of IL-4/Stat6 activities with apoptosis and metastasis in colon cancer. These ...

2008-05-02

307

A Wheat Homolog of MOTHER OF FT AND TFL1 Acts in the Regulation of Germination.

A rat thyrotropin (thyroid-stimulating hormone, TSH) receptor cDNA was isolated that encoded a protein of 764 amino acids, M_r 86,528. Transfection of the cDNA caused COS-7 cells to develop a TSH-sensitive adenylate cyclase response and the ability to bind "1"2"5I-labeled TSH; both activities were similar to those of rat FRTL-5 thyroid cells and not duplicated by lutropin. The gene represented by the cDNA was assigned to mouse chromosome 12 and human chromosome 14. Northern analyses identified two species of mRNA, 5.6 and 3.3 kilobases, in FRTL-5 thyroid cells; the transcripts appeared to differ only in the extent of their 3' noncoding sequences. There were minimal amounts of the two mRNAs in rat ovary, and neither was detected in RNA preparations from rat testis, liver, lung, brain, spleen, and FRT thyroid cells, which do not have a functional TSH receptor. TSH decreased both mRNA species 3- to 4-fold within 8 hr in FRTL-5 thyroid cells; ...

1990-01-01

308

takeout, a Novel Drosophila Gene under Circadian Clock Transcriptional Regulation

Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this ...

2011-09-01

309

Stochastic gene expression and its consequences

We report the identification and characterization of a new Drosophila clock-regulated gene, takeout (to). to is a member of a novel...Full Text Available

2000-09-01

310

Ras activation of genes: Mob-1 as a model.

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

311

Mutation frequency in hprt gene in children accidentally exposed to ionizing radiations; Frequencia de mutacao no gene hprt em criancas acidentalmente expostas as radiacoes ionizantes

The ras oncogenes function by indirectly controlling expression of a subset of yet-undefined genes that are crucial for cell growth and differentiation. In a differential display strategy, numerous...Full Text Available

1994-12-20

312

Molecular cloning and analysis of lymphokines. Volume 13

No abstract prepared

1995-09-01

313

Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male ...

These proceedings collect papers on the subject of lymphokines. Topics include: DNA-cloning of mouse and human lymphokine genes, inteferons, interleukins, gene expression, tumor necrosis factors, and recombinant DNA.

1987-01-01

314

Hormonal Control of Cell Proliferation Requires PASTICCINO Genes

... 041889 Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male Reproducti...

315

Gpnmb is a Melanoblast-Expressed, MITF-Dependent Gene

PASTICCINO (PAS) genes are required for coordinated cell division and differentiation during plant development. In loss-of-function pas mutants,...Full Text Available

2003-07-01

316

Genomics of human longevity

SUMMARYExpression profile analysis clusters Gpnmb with known pigment genes, Tyrp1, Dct, and Si. During development,...Full Text Available

2009-02-01

317

Gene therapy for ocular diseases

In animal models, single-gene mutations in genes involved in insulin/IGF and target of rapamycin signalling pathways extend lifespan to a considerable extent. The genetic, genomic and epigenetic influences...Full Text Available

2011-01-12

318

Cloning of the neurodegeneration gene drop-dead and characterization of additional phenotypes of its mutation

The eye is an easily accessible, highly compartmentalised and immune-privileged organ that offers unique advantages as a gene therapy target. Significant advancements have been made in understanding...Full Text Available

2011-05-01

319

Cis-regulatory mutations in human disease

Mutations in the Drosophila gene drop-dead (drd) result in early adult lethality and neurodegeneration, but the molecular identity of the drd...Full Text Available

2008-01-01

320

Analysis of gene TP53 in samples of ocular epithelium in patients with senile cataract; Analise do gene TP53 em amostras de epitelio ocular obtidas de pacientes com catarata senil

Cis-acting regulatory sequences are required for the proper temporal and spatial control of gene expression. Variation in gene expression is highly heritable and a significant determinant...Full Text Available

2009-07-01

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321

SUPPLEMENTAL COLUMBIA RIVER PROTECTION ACTIVITIES AT THE DEPARTMENT OF ENERGY HANFORD SITE: 2006 TECHNICAL PEER REVIEW

No abstract prepared.

2000-09-01

322

Climate change in the Cairns and Great Barrier Reef region. Scope and Focus for an Integrated Assessment

Prompted by a $10 million Congressional allocation to identify supplemental actions to protect the Columbia River from groundwater contamination beneath the Hanford Reservation, the U. S. Department of Energy (DOE) Environmental Management (EM) Office of Clean-up Technology identified twenty-three potential technical projects and then down-selected ten of these for further evaluation. An independent expert peer review was conducted for the ten down-selected proposals. The review panel consisted of twenty-three recognized subject matter experts that broadly represented academia, industry, and federal laboratories. Of the initial ten proposals reviewed, one was given unconditional support, six were given conditional support, and three were not supported as proposed. Three additional proposals were then submitted by DOE for review--these proposals were structured, in part, to respond to the initial round of technical peer ...

2006-12-20

323

Attack Methodology Analysis: Emerging Trends in Computer-Based Attack Methodologies and Their Applicability to Control System Networks

This study was undertaken to determine the scope and focus for an integrated assessment of climate change impacts on, and adaptation options for, the Cairns Great Barrier Reef (CGBR) region. To achieve this, the authors employed both technical expertise and regional stakeholder input. This document describes the study objectives and the process used to meet these objectives, and provides an overview of the CGBR region, the views of technical experts on potential climate change impacts, stakeholder prioritisation of impacts and adaptation options, a list of perceived knowledge gaps, and a recommended structure for a future integrated assessment in the region. The aim of the study was to determine the scope and focus for an integrated regional assessment of climate change impacts on, and adaptation options for, the CGBR region. The key objectives of the study were: Define and describe the study region; Develop a process for the study, which includes key stakeholders ...

324

Testing the larval drift hypothesis in the Baltic Sea: retention versus dispersion caused by wind-driven circulation

Threat characterization is a key component in evaluating the threat faced by control systems. Without a thorough understanding of the threat faced by critical infrastructure networks, adequate resources cannot be allocated or directed effectively to the defense of these systems. Traditional methods of threat analysis focus on identifying the capabilities and motivations of a specific attacker, assessing the value the adversary would place on targeted systems, and deploying defenses according to the threat posed by the potential adversary. Too many effective exploits and tools exist and are easily accessible to anyone with access to an Internet connection, minimal technical skills, and a significantly reduced motivational threshold to be able to narrow the field of potential adversaries effectively. Understanding how hackers evaluate new IT security research and incorporate significant new ideas into their own tools provides ...

2005-06-01

325

Environmental, health, and safety issues of sodium-sulfur batteries for electric and hybrid vehicles. Volume 1, Cell and battery safety

Retention or dispersion of larvae from the spawning grounds has been identified as one of the key processes influencing recruitment success in fish stocks. To examine the potential effects of transport on recruitment. numerical simulations were performed utilizing a three-dimensional physical oceanographic model of the Baltic Sea. Cod larvae were represented as Lagrangian drifters released in the deepwater region of the Bornholm Basin, the main spawning ground for Baltic cod. Simulations were performed for the major spawning seasons of 1993 and 1994. when annual and interannual variability of meteorological forcing was large. The principal goals of the modelling exercise were first to identify the physical processes influencing the demersal distribution of the early life stages and second to describe the transport of the pelagic stages in response to variations in windstress, thereby identifying the ...

2001-01-01

326

Environmental, health, and safety issues of sodium-sulfur batteries for electric and hybrid vehicles

This report is the first of four volumes that identify and assess the environmental, health, and safety issues involved in using sodium-sulfur (Na/S) battery technology as the energy source in electric and hybrid vehicles that may affect the commercialization of Na/S batteries. This and the other reports on recycling, shipping, and vehicle safety are intended to help the Electric and Hybrid Propulsion Division of the Office of Transportation Technologies in the US Department of Energy (DOE/EHP) determine the direction of its research, development, and demonstration (RD&D) program for Na/S battery technology. The reports review the status of Na/S battery RD&D and identify potential hazards and risks that may require additional research or that may affect the design and use of Na/S batteries. This volume covers cell design and engineering as the basis of safety for Na/S batteries and describes and assesses the ...

1992-09-01

327

cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica

This report is the first of four volumes that identify and assess the environmental, health, and safety issues involved in using sodium-sulfur (Na/S) battery technology as the energy source in electric and hybrid vehicles that may affect the commercialization of Na/S batteries. This and the other reports on recycling, shipping, and vehicle safety are intended to help the Electric and Hybrid Propulsion Division of the Office of Transportation Technologies in the US Department of Energy (DOE/EHP) determine the direction of its research, development, and demonstration (RD D) program for Na/S battery technology. The reports review the status of Na/S battery RD D and identify potential hazards and risks that may require additional research or that may affect the design and use of Na/S batteries. This volume covers cell design and engineering as the basis of safety for Na/S batteries and describes and assesses the ...

1992-09-01

328

cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica

A {lambda}gt11 cDNA library was constructed from poly(U)-Spharose-selected Entamoeba histolytica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit polyclonal anti-E. histolytica serum. A 700-base-pair cDNA insert was isolated and the nucleotide sequence was determined. The deduced amino acid sequence of the cDNA revealed a cysteine-rich protein. DNA hybridizations showed that the gene was specific to E. histolytica since the cDNA probe reacted with DNA from four axenic strains of E. histolytica but did not react with DNA from Entamoeba invadens, Acanthamoeba castellanii, or Trichomonas vaginalis. The insert was subcloned into the expression vector pGEX-1 and the protein was expressed as a fusion with the C terminus of glutathione S-transferase. Purified fusion protein was used to generate 22 monoclonal antibodies (mAbs) and a mouse polyclonal antiserum specific for the E. histolytica portion ...

1990-08-01

329

Breckinridge Project, initial effort. Report VII, Volume III. Cultural resource assessment socioeconomic background data

A #lambda#gt11 cDNA library was constructed from poly(U)-Spharose-selected Entamoeba histolytica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit polyclonal anti-E. histolytica serum. A 700-base-pair cDNA insert was isolated and the nucleotide sequence was determined. The deduced amino acid sequence of the cDNA revealed a cysteine-rich protein. DNA hybridizations showed that the gene was specific to E. histolytica since the cDNA probe reacted with DNA from four axenic strains of E. histolytica but did not react with DNA from Entamoeba invadens, Acanthamoeba castellanii, or Trichomonas vaginalis. The insert was subcloned into the expression vector pGEX-1 and the protein was expressed as a fusion with the C terminus of glutathione S-transferase. Purified fusion protein was used to generate 22 monoclonal antibodies (mAbs) and a mouse polyclonal antiserum specific for the E. histolytica portion ...

330

Working paper on candidate mining sites for surface mining of western oil shales

This report has been prepared in conjunction with an environmental baseline study for a commercial coal conversion facility being conducted by Ashland Synthetic Fuels, Inc. (ASFI) and Airco Energy Company (AECO). This report represents a cultural resource assessment for the proposed plant site and two potential solid waste disposal areas. This assessment presents data collected by Dames and Moore during a recent archaeological reconnaissance of the unsurveyed southeastern portion of the proposed plant site and two potential solid waste disposal areas. Also, results of two previous surveys on the northern and southwestern portion of the plant site for American Smelting and Refining Company (ASARCO) and Kentucky Utilities are included. The Dames and Moore survey of the southeastern portion of the plant site identified one archaeological site, three standing structures and one historic cemetery. In addition 47 archaeological ...

331

Salt repository project closeout status report

This report describes the selection of candidate surface mining sites for the development of oil shale resources in the western United States. The purpose of this study is to input the various scenarios into an economic mine model developed for underground oil shale mines in an earlier project. Site selection was a three-phase effort. Phase I consisted of a thorough review of available resource data, which resulted in a definition of surface mineable areas. Phase II was a detailed investigation of the regions identified in the initial phase. The end result of this task was a list of potential sites having favorable overburden, grade, shale thickness, and topography values. Phase III identified those sites from which a wide range of site specific parameters can be obtained for mine design and model testing purposes. This report describes the procedures used to obtain the candidate mining scenarios. Section 2.0 discusses the ...

1981-09-02

332

Promise and Possibility in Special Education Services for Students with Emotional or Behavioral Disorders: Peacock Hill Revisited

This report provides an overview of the scope and status of the US Department of Energy (DOE`s) Salt Repository Project (SRP) at the time when the project was terminated by the Nuclear Waste Policy Amendments Act of 1987. The report reviews the 10-year program of siting a geologic repository for high-level nuclear waste in rock salt formations. Its purpose is to aid persons interested in the information developed during the course of this effort. Each area is briefly described and the major items of information are noted. This report, the three salt Environmental Assessments, and the Site Characterization Plan are the suggested starting points for any search of the literature and information developed by the program participants. Prior to termination, DOE was preparing to characterize three candidate sites for the first mined geologic repository for the permanent disposal of high-level nuclear waste. The sites were in Nevada, a site in volcanic tuff; Texas, a site in bedded salt ...

1988-06-01

333

Investigation of xenotropic murine leukemia virus-related virus (XMRV) in human and other cell lines.

This article provides an historical look at how programs and practices for students with emotional or behavior disorders (E/BD) have been evaluated since 1964, leading to a codified, although not universally recognized, set of recommendations for evaluating best practices for students with E/BD set out by The Peacock Hill Working Group (1991). The authors contend that, in addition to the programmatic features of best practice, the addition of the quality indicators and standards for research in special education add a critical dimension for examining the quality of the scientific research of identified best practices. Taken together, these set of recommendations and quality indicators represent the state of the art in program evaluation for best practice and programs for students with E/BD. The goal of this article is to outline the current recommendations and apply them to current behavior intervention practices to identify how well the field ...

2010-08-01

334

Energy policy in Taiwan: historical developments, current status and potential improvements

Xenotropic murine leukemia virus-related virus (XMRV) was discovered in human prostate tumors and later in some chronic fatigue syndrome (CFS) patients. However, subsequent studies have identified various sources of potential contamination with XMRV and other murine leukemia virus (MLV)-related sequences in test samples. Biological and nucleotide sequence analysis indicates that XMRV is distinct from known xenotropic MLVs and has a broad host range and cell tropism including human cells. Therefore, it is prudent to minimize the risk of human exposure to infection by evaluating XMRV contamination in cell lines handled in laboratory research and particularly those used in the manufacture of biological products. Nested DNA PCR assays were optimized for investigating XMRV gag and env sequences in various cell lines, which included MRC-5, Vero, HEK-293, MDCK, HeLa, and A549, that may be used in the development of some vaccines and other cell lines ...

2011-10-11

335

Effects of sediment quality on macroinvertebrates in the Sunraysia region of the Murray-Darling Rivers, Australia

Recognizing the importance of energy as a vital component in economic development, the Taiwanese government has been continuously revising its energy policy, seeking to balance economic development, energy supply, and environmental protection (3E). Some measures, in an attempt to achieve the 3E balance, were previously implemented in Taiwan; nevertheless, some unresolved issues departing from certain core principles of Taiwan's sustainable energy policy and an international initiative for a low carbon society remain. The aim of this paper is to examine the energy supply and demand structure of Taiwan and the present status of individual energy carriers (including coal, petroleum, natural gas and electricity). In addition, it investigates the current energy policy framework and its implementation in Taiwan, identifies unresolved issues regarding sustainable energy development, and formulates key policy solutions for certain identifiable problems ...

336

Forest genetics: research and application in Indian forestry

A field-based microcosm approach was tested to identify deterioration of sediment quality in waterways using freshwater macroinvertebrates. The method can potentially identify the nature of contaminants based on species-specific responses. Sediments were collected from the Murray and Darling Rivers and irrigation drains within the Sunraysia region of south-eastern Australia and compared to non-polluted reference sediment. Clean sediments were also spiked with fertiliser to test whether nutrients affected the aquatic fauna. Seven of the eight sediments from the Sunraysia region had a negative impact on the macroinvertebrates, in particular sediment from the Darling River, which supported an impoverished fauna. Three species of chironomid showed varied responses to sediment quality and, although it was hypothesised that nutrients may have impacted on the macroinvertebrate fauna, the results suggest that other pollutants are ...

2008-12-01

337

Synthesis and cloning of the genes of antisense peptides of human calcitonin and miniproinsulin

Sufficient genetic diversity exists in Indian tree species to give large genetic gains. Improvement potential, using wild gene resources for selection and breeding is vast as there are over 244 genera and 400 forest tree species in India. Of these over 30 species and 22 genera are of outstanding forestry value. Selective genetic-variation can be lifted from wild populations, multiplied vegetatively or by seed, and planted with controlled germplasm sources. Among the Indian species no less than 74 reproduce by cutting, 11 by layers, 9 by grafting and buding and 104 by root suckers. More than 161 species coppice vigorously. Afforestation techniques of over 170 species have been worked out. Tree genetic approach of survey of natural variability, quick evaluation, selection, conservation multiplication and planting superior variability directly in the field can give immediate genetic gains. This article discusses genetic information on indigenous ...

1981-04-01

338

SIMPLE METHOD FOR SIMULTANEOUSLY INHIBITING FUNCTION OF MULTIPLE GENE IN NEMATODE

With the aim of an experimental check on the validity of the theory of molecular recognition, the authors have carried out the chemical-enzymatic synthesis and cloning of the gene of human calcitonin and also of the genes of antisense polypeptides to human calcitonin and miniproinsulin. It has been shown that recombinant plasmids obtained on the basis of these synthetic genes are capable of ensuring the biosynthesis of the given polypeptides in E. coli cells as hybrid proteins with the IgG-binding domain of staphylococcal protein A.

1994-07-20

339

J-STORE (Japan)

Full Text Available

2009-10-09

340

Promoter Structure Which Affects on the Expression of Yeast MGMT Gene

Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase.

Full Text Available

1997-01-01

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341

Molecular Cloning and Characterization of the Yew Gene Encoding Squalene Synthase from Taxus cuspidata

A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named...Full Text Available

1991-10-01

342

Investigation of the Association between Normal-tension Glaucoma and Single Nucleotide Polymorphisms in Natriuretic Peptide Gene

Full Text Available

2007-09-01

343

Investigation of Coat Color Candidate Genes in Korean Cattle(Hanwoo)

Full Text Available

344

Immunohistochemical expression of p63 in human prenatal tooth primordia

Full Text Available

2007-12-01

345

Hybridization with synthetic oligonucleotides

development, histochemical, human, malformation, p63 gene, tooth

2005-01-01

346

Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or heteromeric artificial miRNA targeting AP1 and CAL genes

Procedures are described for the use of synthetic oligonucleotides for Southern blot experiments and gene bank screening, and the effect of various mismatches on the efficiency of hybridization is demonstrated. The following topics are discussed: sensitivity vs. specificity, hybridization of a 12-mer to the lambda endolysin gene; hybridization of oligonucleotide probes to the E. coli lac operator; hybridization of synthetic probes to the CYC1 gene of yeast; and cloning eucaryotic genes. (HLW)

1978-01-01

347

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.

Gene silencing is a useful technique for elucidating biological function of genes by knocking down their expression. A recently developed artificial microRNAs (amiRNAs) exploits an endogenous...Full Text Available

2009-03-01

348

GENE EXPRESSION VECTOR USING INSULATOR ORIGINATED FROM SEA URCHIN

We have isolated the human prointerleukin 1 (proIL-1) beta gene from leukocyte and fetal liver libraries. The nucleotide sequence and its gene organization reveals that the proIL-1 beta gene is composed...Full Text Available

1986-10-24

349

J-STORE (Japan)

Full Text Available

2010-03-05

350

Delineation of the ADULT syndrome phenotype due to arginine 298 mutations of the p63 gene

DNA, Genes and Chromosomes

Udgivelsesdato: 2006/8

2006-01-01

351

Construction of the Transgenic Drosophila melanogaster Expressing a Human Megsin Gene.

Today you will learn about the parts of DNA and what DNA, genes and chromosomes are. Today you will learn what DNA, genes and chromosomes are and the parts of the DNA molecule. Look at all of the websites, take whatever notes you need to. At the end of the assignment, be able to describle DNA, the parts of DNA, genes and chromosomes. Covers Biology Core Curriculum, ...

2007-11-07

352

Construction and Verification of Useful Vectors for Ectopic Expression and Suppression of Plant Genes.

Full Text Available

2007-05-01

353

Cell Cycle-dependent Expression of Chitin Synthase Genes in Aspergillus nidulans

Full Text Available

2009-06-01

354

Analysis of the Relationship between MHC-DRB1 Gene Polymorphism and Hydatidosis in Kazakh Sheep

Full Text Available

2001-03-01

355

Chemopreventive effects of Furan-2-yl-3-pyridin-2-yl-propenone against 7,12-dimethylbenz[a]anthracene-inducible genotoxicity

Full Text Available

2010-09-01

356

Association and expression study of synapsin III and schizophrenia.

1-Furan-2-yl-3-pyridin-2-yl-propenone (FPP-3) is an anti-inflammatory agent with a propenone moiety and chemically synthesized recently. In this study, we examined the chemopreventive effect of FPP-3 on 7,12-dimethylbenz[a]anthracene (DMBA)-induced genotoxicity in MCF-7 cells. FPP-3 reduced the formation of the DMBA-DNA adduct. DMBA-induced CYP1A1 and CYP1B1 gene expression and enzyme activity were inhibited by FPP-3. It inhibited DMBA-induced aryl hydrocarbon receptor (AhR) transactivation and DMBA-inducible nuclear localization of the AhR. Induction of detoxifying phase II genes by chemopreventive agents represents a coordinated protective response against oxidative stress and neoplastic effects of carcinogens. Transcription factor NF-E2 related factor 2 (Nrf2) regulates antioxidant response element (ARE) of phase II detoxifying and antioxidant enzymes, such as glutathione S-transferase (GST) and NAD(P)H:quinone oxidoreductase (QR). FPP-3 ...

2008-05-01

357

Antisense-mediated suppression of C-hordein biosynthesis in the barley grain results in correlated changes in the transcriptome, protein profile, and amino acid composition

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter release and in synaptogenesis, suggesting a potential role in several neuropsychiatric diseases. The human SYN3 gene is located on chromosome 22q12-13, a candidate region implicated in previous linkage studies of schizophrenia. However, association studies of SYN3 and schizophrenia have produced inconsistent results. In this study, four SYN3 SNPs (rs133945 (-631 C>G), rs133946 (-196 G>A), rs9862 and rs1056484) were tested in three sets of totally 3759 samples that comprise 655 affected subjects and 626 controls in the Irish Case-Control Study of Schizophrenia (ICCSS), 1350 samples incorporating 273 pedigrees in the Irish Study of High Density Schizophrenia Families (ISHDSF), and 564 unrelated schizophrenia patients and 564 healthy individuals in a Chinese ...

2009-09-19

358

Antisense-mediated suppression of C-hordein biosynthesis in the barley grain results in correlated changes in the transcriptome, protein profile and amino acid composition

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition. The antisense line had a lower C-hordein level and down-regulated transcript encoding C-hordein. The production of the S-rich B/gamma- and ...

2007-01-01

359

S1 nuclease analysis of #alpha#-globin gene expression in preleukemic patients with acquired hemoglobin H disease after transfer to mouse erythroleukemia cells

Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition. The antisense line had a lower C-hordein level and down-regulated transcript encoding C-hordein. The production of the S-rich B/ - and D-hordeins ...

2007-01-01

360

The neuroimmune-endocrine axis: pathophysiological implications for the central nervous system cytokines and hypothalamus-pituitary-adrenal hormone dynamics

The loss of #alpha#-globin gene transcriptional activity rarely occurs as an acquired abnormality during the evolution of myeloproliferative disease or preleukemia. To test whether the mutation responsible for the loss of #alpha#-globin gene expression (hemoglobin H disease) in these patients is linked with the #alpha#-globin genes on chromosome 16, the authors transferred chromosome 16 from preleukemic patients with acquired hemoglobin H disease to mouse erythroleukemia cells and measured the transcriptional activity of the human #alpha#-globin genes. After transfer to mouse erythroleukemia cells, the expression of human #alpha#-globin genes from the peripheral blood or marrow cells of preleukemic patients with acquired hemoglobin H disease was similar to that of human #alpha#-globin genes transferred to mouse erythroleukemia cells from normal donors. These ...

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361

Scientific Electronic Library Online (English)

Abstract in english Cytokines are molecules that were initially discovered in the immune system as mediators of communication between various types of immune cells. However, it soon became evident that cytokines exert profound effects on key functions of the central nervous system, such as food intake, fever, neuroendocrine regulation, long-term potentiation, and behavior. In the 80's and 90's our group and others discovered that the genes encoding various cytokines and their receptors are e (more) xpressed in vascular, glial, and neuronal structures of the adult brain. Most cytokines act through cell surface receptors that have one transmembrane domain and which transduce a signal through the JAK/STAT pathway. Of particular physiological and pathophysiological relevance is the fact that cytokines are potent regulators of hypothalamic neuropeptidergic systems that maintain neuroendocrine homeostasis and which regulate the body's response to ...

2000-10-01

362

Systems Biology Approach in Chlamydomonas Reveals Connections between Copper Nutrition and Multiple Metabolic Steps[C][W][OA

A novel drug and gene co-delivery system based on Poly(epsilon-caprolactone)-Poly(ethylene glycol)-Poly(epsilon-caprolactone) grafted polyethyleneimine micelle.

In this work, we query the Chlamydomonas reinhardtii copper regulon at a whole-genome level. Our RNA-Seq data simulation and analysis pipeline validated a 2-fold cutoff and 10 RPKM (reads per kilobase of mappable length per million mapped reads) (~1 mRNA per cell) to reveal 63 CRR1 targets plus another 86 copper-responsive genes. Proteomic and immunoblot analyses captured 25% of the corresponding proteins, whose abundance was also dependent on copper nutrition, validating transcriptional regulation as a major control mechanism for copper signaling in Chlamydomonas. The impact of copper deficiency on the expression of several O2-dependent enzymes included steps in lipid modification pathways. Quantitative lipid profiles indicated increased polyunsaturation of fatty acids on thylakoid membrane digalactosyldiglycerides, indicating a global impact of copper deficiency on the photosynthetic apparatus. Discovery of a putative plastid copper chaperone and a membrane ...

2011-04-01

363

Tracheal compression by mediastinal masses in children: CT evaluation

In this paper, we prepared a novel cationic self-assembled micelle from poly(epsilon-caprolactone)-poly(ethyl glycol)-poly(epsilon-caprolactone) grafted polyethyleneimine (PCEC-g-PEI). The PCEC-g-PEI micelles, formed by self-assembly method, had mean particle size of ca. 82 nm and zeta potential of +22.5 mV at 37 degrees C, and could efficiently transfer pGFP into HEK293 cells in vitro. Meanwhile, as a model hydrophobic chemotherapeutic drug, honokiol was loaded into PCEC-g-PEI micelles by direct dissolution method assisted by ultrasonication. The honokiol loaded cationic PCEC-g-PEI micelles could effectively adsorb DNA onto its surface, while it could release honokiol in an extended period in vitro. This study demonstrated a novel DNA and hydrophobic chemotherapeutic drug co-delivery system. PMID:21121283

2010-12-01

364

Toward a hybrid data mining model for customer retention

Chest computed tomography (CT) was valuable in detecting extrinsic tracheal compression by mediastinal masses in two pediatric patients. This prompted an independent evaluation by CT of 14 children with masses involving the middle mediastinum and possible intrathoracic tracheal narrowing. Computer programs permit precise calculation of tracheal cross-sectional areas. Any apparent decrease in tracheal area may be compared with CT-derived data in normal children. Chest CT not only demonstrates the presence of extrinsic airway compression in pediatric patients with mediastinal masses, but also is capable of precisely measuring the extent of this narrowing. This method identifies children at potential risk for respiratory compromise and may aid in subsequent therapy.

1983-10-01

365

British Library Electronic Table of Contents (United Kingdom)

The prevention of subscriber churn through customer retention is a core issue of Customer Relationship Management (CRM). By minimizing customer churn a company maximizes its profit. This paper proposes a hybridized architecture to deal with customer retention problems. It does so not only through predicting churn probability but also by proposing retention policies. The architecture works in two modes: learning and usage.In the learning mode, the churn model learner seeks potential associations from the subscriber database. This historical information is used to form a churn model. This mode also calls for a policy model constructor to use the attributes identified in the churn model to divide all `churners' into distinct groups. The policy model constructor is also responsible for develop...

2007-01-01

366

Targeting the kynurenine pathway as a potential strategy to prevent and treat Alzheimer's disease

British Library Electronic Table of Contents (United Kingdom)

Alzheimer's disease (AD) is a progressive neurodegenerative disorder of the elderly accounting for the vast majority of dementia. Recently, many studies have implicated the role of inflammatory response, especially neuroinflammatory response in the development and progression of AD. However, the underlying mechanism of how inflammatory response induces AD is unknown. Kynurenine pathway is a major route of the amino acid tryptophan catabolism, resulting in the production of nicotine adenine dinucleotide and other neuroactive intermediates: quinolinic acid (QA) and kynurenic acid (KA). QA exerts different toxic effects, including over-activation of N-methyl-d-aspartate (NMDA) receptor and excitotoxicity, synaptic dysfunction and neuronal death. On the other hand, KA is identified as the only...

2011-01-01

367

Status of the surry low power and shutdown PRA

Stabilization of geothermal residues by encapsulation in portland cement-based composites

The Surry low power and shutdown probabilistic risk analysis (PRA) is an ongoing project at Brookhaven National Laboratory (BNL) to identify and quantify potential accident scenarios that may occur in a pressurized water reactor (PWR) during low power and shutdown. It was initiated as a result of various incidents and accidents that have occurred within the United States and overseas. The project involves review and evaluation of PWR experience at shutdown, identification of accident scenarios, determination of methods to mitigate the accidents, and performance a level 1 PRA. An evaluation of accident progression, source terms and consequences has also been initiated. The results will be used to address issues related to shutdown conditions. The objective of this paper is to provide a progress report on the project, and to present the approach used as well as the preliminary results of the ongoing and completed tasks.

1991-04-01

368

Spatio-temporal variability of precipitation, temperature and agricultural drought indices in Central Italy

Presented are the results from a laboratory test program conducted to identify and evaluate materials for converting hazardous geothermal residues to a non-hazardous and potentially usable form. Results indicate that the residues can be effectively incorporated, as a fine aggregate, into portland cement-based composites. Five geothermal residues obtained from sites in the Salton Sea area of California were evaluated. Three of these were classified as hazardous. After mixing with cement, the leach rates were all well below specified levels. Although structural-grade composites were produced, gradual reductions in properties with time up to 1 yr were noted. This indicates ongoing chemical reactions between the cement paste and the constituents of the residues. Further research is necessary before the composites could be considered for use in structural applications. 3 refs., 8 tabs.

1988-05-01

369

British Library Electronic Table of Contents (United Kingdom)

The agricultural sector is probably the one that will suffer most directly from the climatic variations expected at the global level. In particular, the analysis of the changes expected in water availability and demand is fundamental in order to correctly establish both the present water resource management and the definition of new strategies. In this paper the time series of some climatic and agro-climatic indices in the Region of Umbria (Central Italy) have been analyzed with the aim of finding signs of climate changes and identifying the potential impacts on the agricultural water balance. The aforesaid indices include the precipitation, the mean maximum and minimum temperatures (Tmin, Tmax), the mean temperature range (DT), the reference evapotranspiration (ET0) and two drought indice...

2011-01-01

370

Soluble starch?based biodegradable and microporous microspheres as potential adsorbent for stabilization and controlled release of coix seed oil

British Library Electronic Table of Contents (United Kingdom)

Soluble starch?based biodegradable and microporous microspheres (SDM-Ms) were prepared by emulsion chemical cross-linking technique using trisodium trimetaphosphate (TSTP) as the cross-linker. The resultant amorphous SDM-Ms were excellently identified by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). The internal rupture and surface-controlled erosion were the predominant degradation mechanisms for SDM-Ms. The obtained SDM-Ms were applied to adsorb coix seed oil (CSO) by immersing method. The adsorption capacity of CSO within SDM-Ms was determined, namely 0.5238?g/g, and the thermal stability was shown higher than that of the free CSO. A burst release appeared in the second hour, followed by controlled release. Diffusion, d...

2011-01-01

371

Serotonin Inhibits Protein Feeding in the Blow Fly, Phormia regina (Meigen)

British Library Electronic Table of Contents (United Kingdom)

Serotonin is an important signaling molecule involved in the control of feeding in flies and other animals. In this study, a potential neurohemal release site for serotonin and the effects of exogenous serotonin on protein feeding were examined in the black blow fly, Phormia regina. A dense network of varicose neural processes exhibiting serotonin-like immunoreactivity was identified on the dorsal region of the thoracico-abdominal ganglion in P. regina. This dorsal region of the central nervous system is a likely site for the release of serotonin into the hemolymph. Circulating serotonin may have multiple systemic effects on fly physiology, including modulating or regulating feeding related processes and diuresis. Injections of exogenous serotonin reduced protein meal size in female flies ...

2009-01-01

372

Selection of IFE target materials from a safety and environmental perspective

Seasonal and habitat abundance and distribution of some forensically important blow flies (Diptera: Calliphoridae) in Central California

Target materials for inertial fusion energy (IFE) power plant designs might be selected for a wide variety of reasons including wall absorption of driver energy, material opacity, cost and ease of fabrication. While each of these issues are of great importance, target materials should also be selected based upon their safety and environmental (S and E) characteristics. The present work focuses on the recycling, waste management and accident dose characteristics of potential target materials. If target materials are recycled so that the quantity is small, isotopic separation may be economically viable. Therefore, calculations have been completed for all stable isotopes for all elements from lithium to polonium. The results of these calculations are used to identify specific isotopes and elements that are most likely to be offensive as well as those most likely to be acceptable in terms of their S and E characteristics.

2001-05-21

373

British Library Electronic Table of Contents (United Kingdom)

Seasonal and habitat calliphorid abundance and distribution were examined weekly for two years (2001-2003) in Santa Clara County, California, using sentinel traps baited with bovine liver. Of the 34,389 flies examined in three defined habitats (rural, urban, and riparian), 38% of the total catch represented Compsomyiops callipes (Bigot) and 23% represented Phormia regina (Meigen). Other flies collected in this survey included Calliphora vomitoria (Linnaeus), Calliphora latifrons (Hough), Lucilia sericata (Meigen), Lucilia cuprina (Wiedemann), and Lucilia mexicana (Macquart), which is a new record for the area. Multivariate MANOVA and ANOVA (P 0.05) analysis indicate significant seasonal habitat preference for all fly species examined. This information may be used to identify potentially fo...

2011-01-01

374

Reductive metabolism of the dinitrobenzamide mustard anticancer prodrug PR-104 in mice

British Library Electronic Table of Contents (United Kingdom)

Purpose PR-104, a bioreductive prodrug in clinical trial, is a phosphate ester which is rapidly metabolized to the corresponding alcohol PR-104A. This dinitrobenzamide mustard is activated by reduction to hydroxylamine (PR-104H) and amine (PR-104M) metabolites selectively in hypoxic cells, and also independently of hypoxia by aldo-keto reductase (AKR) 1C3 in some tumors. Here, we evaluate reductive metabolism of PR-104A in mice and its significance for host toxicity. Methods The pharmacokinetics of PR-104, PR-104A and its reduced metabolites were investigated in plasma and tissues of mice (with and without SiHa or H460 tumor xenografts) and effects of potential oxidoreductase inhibitors were evaluated. Results Pharmacokinetic studies identified extensive non-tumor reduction of PR-104A to t...

2011-01-01

375

Recognizing limitations in eddy current testing

Promoting medical tourism to India: Messages, images, and the marketing of international patient travel

This paper addresses known limitations and constraints in eddy current nondestructive testing. Incomplete appreciation for eddy current limitations is believed to have contributed to both under-utilization and misapplication of the technique. Neither situation need arise if known limitations are recognized. Some, such as the skin depth effect, are inherent to electromagnetic test methods and define the role of eddy current testing. Others can be overcome with available technology such as surface probes to find circumferential cracks in tubes and magnetic saturation of ferromagnetic alloys to eliminate permeability effects. The variables responsible for limitations in eddy current testing are discussed and where alternative approaches exist, these are presented. Areas with potential for further research and development are also identified.

1993-11-01

376

British Library Electronic Table of Contents (United Kingdom)

The practice of medical tourism depends on successfully informing potential patients about procedure options, treatment facilities, tourism opportunities, travel arrangements, and destination countries. The promotion of medical tourism includes a wide range of marketing materials such as flyers, booklets, and websites. Yet, there is a paucity of knowledge about the dissemination, content, and reception of these promotional materials. Drawing on a thematic content analysis of the promotional print material distributed at the first medical tourism trade show in Canada in 2009, the main purpose of this article is to identify and understand the messages and images that companies use to market India as a global destination. While researchers and news media frequently cite low cost procedures as...

2011-01-01

377

Production of {sup 62}Zn, {sup 65}Zn and {sup 203}Pb radioisotopes for studying the transport of zinc and lead in plants

Production of "6"2Zn, "6"5Zn and "2"0"3Pb radioisotopes for studying the transport of zinc and lead in plants

In the Carpathian Basin, significant percentage of watershed area and floodplains of rivers are utilised agriculturally. Several potential sources of poisonous metal pollution have been identified in these areas. Because of spills from some of them a few severe accidents have happened especially in the watershed area of Tisza River during the last decades. The motivation of our present work was to produce {sup 62,65}Zn and {sup 203}Pb radioisotopes because they can be used especially as tracers for studying the kinetics of uptake, transport and accumulation of zinc and lead by plants under different circumstances. (orig.)

2004-07-01

378

PROSPECTS AND STATUS OF LOW-ASPECT-RATIO TOKAMAKS

In the Carpathian Basin, significant percentage of watershed area and floodplains of rivers are utilised agriculturally. Several potential sources of poisonous metal pollution have been identified in these areas. Because of spills from some of them a few severe accidents have happened especially in the watershed area of Tisza River during the last decades. The motivation of our present work was to produce "6"2","6"5Zn and "2"0"3Pb radioisotopes because they can be used especially as tracers for studying the kinetics of uptake, transport and accumulation of zinc and lead by plants under different circumstances. (orig.)

379

PROBING NEAR-SURFACE ATMOSPHERIC TURBULENCE WITH LIDAR MEASUREMENTS AND HIGH-RESOLUTION HYDRODYNAMIC MODELS

The prospects for the low-aspect-ratio (A) tokamak to fulfill the requirements of viable fusion power plants are considered relative to the present status in data and modeling. Desirable physics and design features for an attractive Blanket Test Facility and power reactors are estimated for low-A tokamaks based on calculations improved with the latest data from small pioneering experiments. While these experiments have confirmed some of the recent predictions for low-A, they also identify the remaining issues that require verification before reliable projections can be made for these deuterium-tritium applications. The results show that the low-A regime of small size, modest field, and high current offers a path complementary to the standard and high A tokamaks in developing the full potential of fusion power.

1995-01-01

380

Oxidation/sulfidation of material candidates for distributed solar receiver thermochemical transport program in SO/sub 2//O/sub 2/

As lidar technology is able to provide fast data collection at a resolution of meters in an atmospheric volume, it is imperative to promote a modeling counterpart of the lidar capability. This paper describes an integrated capability based on data from a scanning water vapor lidar and a high-resolution hydrodynamic model (HIGRAD) equipped with a visualization routine (VIEWER) that simulates the lidar scanning. The purpose is to better understand the spatial and temporal representativeness of the lidar measurements and, in turn, to extend their utility in studying turbulence fields in the atmospheric boundary layer. Raman lidar water vapor data collected over the Pacific warm pool and the simulations with the HIGRAD code are used for identifying the underlying physics and potential aliasing effects of spatially resolved lidar measurements. This capability also helps improve the trade-off between spatial-temporal resolution and coverage of the ...

2000-11-01

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381

Oral administration of purple passion fruit peel extract attenuates blood pressure in female spontaneously hypertensive rats and humans

Metals for potential use in the dissociator and the synthesizer reactors of a distributed solar receiver thermochemical transport loop utilizing SO/sub 3//SO/sub 2//O/sub 2/ molecular chemistry were tested in SO/sub 2//O/sub 2/ mixtures at 500/sup 0/C and 900/sup 0/C, respectively, for times of up to four weeks. They included titanium, aluminum and nickel, and iron-base, nickel-base and cobalt-base superalloys. Weight gain measurements determine the oxidation/sulfidation kinetics. Electron microprobe analysis identifies any possible penetration of oxygen and sulfur into the metal and the formation of internal oxides and sulfides. The most promising candidates as a result of these tests are the cobalt-base superalloys.

1985-06-01

382

British Library Electronic Table of Contents (United Kingdom)

Hypertension is one of the most important modifiable risk factors for cardiovascular and cerebrovascular diseases. We investigated the potential antihypertensive effect of the purple passion fruit peel (PFP) extract, a mixture of bioflavonoids, phenolic acids, and anthocyanins, in spontaneously hypertensive rats and human. A high-performance liquid chromatography analysis was performed to identify the active ingredients of the PFP extract. In a rat liver toxicity assay, no hepatotoxicity was observed after 9 hours incubation in the presence of PFP extract (20 ?g/mL). The PFP extract also revealed hepatoprotection against chloroform (1 mmol/L)-induced liver injury. In the experimental model, 24 spontaneously hypertensive rats were divided into 3 treatment groups for a period of 8weeks: cont...

2007-01-01

383

Occurrences and distributions of branched alkylbenzenes in the Dongsheng sedimentary uranium ore deposits, China

British Library Electronic Table of Contents (United Kingdom)

A series of branched alkylbenzene ranging from C15 to C19 with several isomers (2?5) at each carbon number were identified in sediments from the Dongsheng sedimentary uranium ore deposits, Ordos Basin, China. The distribution patterns of the branched alkylbenzenes show significant differences in the sample extracts. The branched alkylbenzenes from organic-rich argillites and coals range from C15 to C19 homologues, in which the C17 or C18 dominated. On the other hand, the C19 branched alkylbenzenes dominated in the sandstone/siltstone extracts. The obvious differences of the branched alkylbenzene distributions between the uranium-host sandstones/siltstones and the interbedded barren organic-rich mudstones/coals probably indicate their potential use as biological markers associated with part...

2010-01-01

384

Multidate image analysis of forest degradation in equatorial Africa

Magnetic refrigeration: the promise and the problems

A section of the northern margin of the Guineo-Congolian rain forest of the Central African Republic is studied to determine to what extent deforestation is evolving. Three sites are presented to highlight the diversity in local environmental settings at the northern margin of the closed equatorial rain forest: the contiguous equatorial rain forest, the boundary between the closed rain forest and the grasslands, and a predominantly secondary grassland environment. Proven image processing procedures for determining land cover and vegetation vigor were applied to Landsat MSS data to determine land cover characteristics and identify alterations in land cover that indicate potentially degraded forest environments. Land cover was independently assessed using spectral signatures determined from a statistical clustering routine. The images presented and image analyses contribute insights and information to an ongoing effort to determine more reliable ...

1990-12-01

385

Large (≥2cm) non-hypervascular nodules depicted on MRI in the cirrhotic liver: fate and implications

Magnetic refrigeration uses the temperature- and field-dependence of the entropy of some magnetic materials to accomplish cooling. Because of the intrinsically high efficiency of the magnetization and demagnetization process and because of the potential for excellent heat transfer between solids and fluids, magnetic refrigerators promise to have higher efficiency than existing gas-cycle refrigerators. Many ground-based and space-based applications could benefit significantly from the cost savings implied by higher efficiency. Other attributes of these devices are high reliability and low volume and mass per unit cooling power. The development of these refrigerators is underway at several places around the world, including the Los Alamos National Laboratory. The progress to date has been encouraging but some problems have been clearly identified. The arguments for high efficiency and the problems that will need to be solved to achieve this goal ...

1984-01-01

386

British Library Electronic Table of Contents (United Kingdom)

Aim To determine the fate and clinical implication of large (?2cm), non-hypervascular nodules depicted on magnetic resonance imaging (MRI) in the cirrhotic liver. Materials and methods In 21 patients with cirrhosis (14 hepatitis B, two ethanol abuse, four cryptogenic, one Wilson's disease), 25 large (?2cm in the longest dimension) non-hypervascular nodules were identified on dynamic MRI. The implications for diagnosis of the initial size, contour, and signal characteristics on MRI in addition to patients' age and cause of cirrhosis were assessed in our analysis. Results Twelve (75%) out of 16 lesions were malignant or potentially-malignant from 14 hepatitis B patients, while seven (78%) of the nine lesions from other patients were benign (p=0.016). The mean age of the patients who ha...

2008-01-01

387

Is XMRV a causal virus for prostate cancer?

British Library Electronic Table of Contents (United Kingdom)

The potential association between xenotropic murine leukaemia virus-related gammaretrovirus (XMRV) and prostate cancer (PCa) has been documented since 2006. It is important for furthering our understanding of the biological mechanisms of PCa to ascertain whether this association is causal. To summarize the available information on the epidemiological and laboratory findings of the association, we conducted a literature search of the PubMed electronic database (from March 2006 to February 2011) to identify relevant published studies that examined the association between XMRV and PCa. Although several studies showed the positive association between XMRV and PCa, more recent studies did not support this conclusion. The positive findings might be due to contamination of human samples. Further ...

2011-01-01

388

Influence of shape on ordering of granular systems in two dimensions

Impact of Invasive Cane Toads on Australian Birds

We investigate ordering properties of two-dimensional granular materials using several shapes created by welding ball bearings together. Ordered domains form much more easily in two than in three dimensions, even when configurations lack long-range order. The onset of ordered domains occurs near a packing density of 0.8, a phenomenon observed previously for disks. One of our shapes, the trapezoid, has packings that remain disordered and near the transition density even after annealing by shaking. Although random packings are unstable for disks and many other shapes in two dimensions, trapezoid packings provide an approach to studying two-dimensional randomness. We also find that the rotational symmetry of a shape is an excellent predictor of how easily it orders, and a potential guide to identifying two-dimensional shapes that remain random after annealing.

2001-06-01

389

British Library Electronic Table of Contents (United Kingdom)

Abstract: The cane toad (Bufo marinus), a large, toxic, American anuran, was introduced to Australia in 1935. Populations of many of Australia's reptiles (snakes, varanid lizards, crocodiles) and carnivorous mammals (dasyurid marsupials) have declined because these predators are killed by the toad's powerful toxins. In contrast to these well-studied species, little is known about the cane toads impacts on Australian birds. We reviewed published and unpublished data on behavioral interactions between Australian avian predators and cane toads and collated distributional and dietary information to identify avian taxa potentially at risk from cane toad invasion. Cane toads are sympatric with 172 frog-eating bird species in Australia, and an additional 8 bird species overlap with the predicted ...

2009-01-01

390

Google Android: A State-of-the-Art Review of Security Mechanisms

CERN Document Server

Google's Android is a comprehensive software framework for mobile communication devices (i.e., smartphones, PDAs). The Android framework includes an operating system, middleware and a set of key applications. The incorporation of integrated access services to the Internet on such mobile devices, however, increases their exposure to damages inflicted by various types of malware. This paper provides a comprehensive security assessment of the Android framework and the security mechanisms incorporated into it. A methodological qualitative risk analysis that we conducted identifies the high-risk threats to the framework and any potential danger to information or to the system resulting from vulnerabilities that have been uncovered and exploited. Our review of current academic and commercial solutions in the area of smartphone security yields a list of applied and recommended defense mechanisms for hardening mobile devices in general and the Android ...

2009-01-01

391

Fractional domain walls from on-site softening in dipolar bosons

CERN Document Server

We study dipolar bosons in a 1D optical lattice and identify a region in parameter space---strong coupling but relatively weak on-site repulsion---hosting a series of stable CDW states whose low-energy excitations, built from "fractional domain walls", are remarkably similar to those of non-abelian fractional quantum Hall states. Here, a conventional domain wall between translated CDW's may split by inserting strings of degenerate, but inequivalent, CDW states. Outside these insulating regions, we find numerous supersolids as well as a superfluid regime. The mentioned phases should be accessible experimentally, and in particular, the fractional domain walls can be created in the ground state using single-site addressing, i.e. by locally changing the chemical potential.

2011-01-01

392

Fish intake and acute coronary syndrome

British Library Electronic Table of Contents (United Kingdom)

Aims To study the effect of fish consumption on the risk of acute coronary syndrome (ACS) in healthy subjects. Methods and results This Danish follow-up study included 57 053 men and women between 50 and 64 years. Intake of lean and fatty fish was estimated from a detailed and validated food frequency questionnaire. Potential cases of ACS were identified through nationwide medical databases. A total of 1122 cases of ACS were verified during a mean follow-up period of 7.6 years. Among men, intake of fatty fish was associated with a lower risk of ACS. For men in the highest quintile of fish intake compared with the lowest quintile, the hazard ratio was 0.67 (95% confidence interval: 0.53-0.85). The inverse association was observed for intakes >6 g of fatty fish per day with no obvious additi...

2010-01-01

393

Fire and explosion assessment on oil and gas floating production storage offloading (FPSO): An effective screening and comparison tool

British Library Electronic Table of Contents (United Kingdom)

Fires and explosions have been identified as major potential hazards for Oil and Gas Floating Production Storage Offloading (FPSO) installations and pose risk to personnel, assets, and the environment. Current fire and explosion assessment (FEA) tools require physical effect modeling software and follows standards from API, ISO, and engineering practices. However, the tools are not specific to any particular system such as an FPSO, and do not provide comprehensive guidance for safety engineers to perform FEA. This paper discusses the development of a screening and comparison tool for FEA on FPSOs and the incorporation of an expert system into the tool. The results are computerized using MS Excel/VBA to provide a structured and comprehensive assessment on each equipment and module handling ...

2009-01-01

394

Finite element analysis of a scanning x-ray microscope micropositioning stage

A micropositioning stage for use with an x-ray microscope has been developed to scan samples over a 100 {mu}m{times}100-{mu}m area with a resolution of 100 A. The stage employs flexure hinges driven by piezoelectric actuators to achieve this high positioning accuracy. Due to the severe geometry of the hinges, stresses and deflections are determined (via finite element methods) to ensure a credible design. Since the stage is to be used in a stepping capacity, natural frequencies are computed to identify resonant conditions which could potentially cause excessive deflections. The finite element models show that the stage responds like a discrete lumped-mass system for the lower modes, and a continuous system with flexural characteristics for the higher modes.

1992-01-01

395

Explosives sensor

Evolution of drought severity and its impact on corn in the Republic of Moldova

A compact and supersensitive device that can rapidly detect minute trace vapors from concealed explosives has been developed by scientists at Oak Ridge National Laboratory (ORNL). The new explosives sensor can detect and chemically identify organic nitrogen-oxygen compounds which are the building blocks of explosives such as TNT, plastiques, and nitroglycerine. The device could be used to scan persons entering airport terminals, nuclear power plants, defense installations, or other sensitive locations, providing greater security against potential terrorism. This device works on a glow discharge principle, and is more specifically called an ''Atmospheric Sampling Glow Discharge Ionization'' (ASGDI) source. The new detector is a highly automated, miniaturized version of research mass spectrometers widely used to trace constituents of chemical mixtures. Detail of this device's construction and ...

1987-01-01

396

British Library Electronic Table of Contents (United Kingdom)

Droughts in Moldova were evaluated using meteorological data since 1955 and a long time series (1891?2009). In addition, yields for corn (Zea mays L.), a crop widely grown in Moldova, were used to demonstrate drought impact. The main aim is to propose use of the S i (S i-a and S i-m) drought index while discussing its potential use in studying the evolution of drought severity in Moldova. Also, a new multi-scalar drought index, the standardized precipitation?evapotranspiration index (SPEI), is tested for the first time in identifying drought variability in Moldova while comparing it with the commonly used standardized precipitation index (SPI). S i-m, SPI, SPEI, and S i-a indices show an increasing tendency toward more intensive and prolonged severely dry and extremely dry summer months. D...

2011-01-01

397

Evaluation of the Influence That Was Produced by Phytoremediation of Soil Microorganisms at Oil Showings

Evaluation of potential severe accidents during low power and shutdown operations at Surry: Unit 1, Volume 1

Phytoremediation has been identified as a potentially environmentally friendly and cost effective technique for the treatment of contaminated soil. However, phytoremediation has an unknown mechanism. In this study, we focus on the effects of the cultivation of Italian ryegrass on the soil microbes collected at oil showings, which were expected to have a variety of crude oil degradable microorganisms. We evaluated the number of crude oil degradable microorganism, microbial activity, microflora using the PCR-DGGE method and the change in the concentration of crude oil in the soil. The results indicated that the microflora was affected by the cultivation of Itarian ryegrass, and that the microbial activity and the number of crude oil degradable microorganisms were also improved by the cultivation. Moreover, the concentration of crude oil in the rhizosphere soil decreased significantly when compared to the uncultivated soil. These results suggested ...

2009-01-01

398

Energetics of the fission process

This document contains a summarization of the results and insights from the Level 1 accident sequence analyses of internally initiated events, internally initiated fire and flood events, seismically initiated events, and the Level 2/3 risk analysis of internally initiated events (excluding fire and flood) for Surry, Unit 1. The analysis was confined to mid-loop operation, which can occur during three plant operational states (identified as POSs R6 and R10 during a refueling outage, and POS D6 during drained maintenance). The report summarizes the Level 1 information contained in Volumes 2--5 and the Level 2/3 information contained in Volume 6 of NUREG/CR-6144.

1990-10-22

399

Energetics of the fission process

The mass asymmetry of fragments from nuclear fission of heavy nuclei is reviewed. While mass asymmetry is a common and well-known phenomenon for low-energy fission of the lighter actinides, more recent experiments have demonstrated that, for the heaviest actinides, the mass distribution switches to a symmetric one. On the other hand, it has been discovered that, though for fissioning nuclei with mass numbers A< or [approx]225 the mass distribution is basically symmetric, an asymmetric component is clearly to be identified for nuclei down to the Pb-region. In the absence of a generally accepted dynamical theory of fission, the above experimental findings are discussed in terms of static energy considerations. Triggered from the outset by the structure of the potential energy surface at the saddlepoint, the energy balance at the scission point between the available energy (Q-value) of the reaction and the Coulomb and deformation energy of the ...

1994-09-01

400

Earliest art in the Americas: incised image of a proboscidean on a mineralized extinct animal bone from Vero Beach, Florida

The mass asymmetry of fragments from nuclear fission of heavy nuclei is reviewed. While mass asymmetry is a common and well-known phenomenon for low-energy fission of the lighter actinides, more recent experiments have demonstrated that, for the heaviest actinides, the mass distribution switches to a symmetric one. On the other hand, it has been discovered that, though for fissioning nuclei with mass numbers A< or #approx#225 the mass distribution is basically symmetric, an asymmetric component is clearly to be identified for nuclei down to the Pb-region. In the absence of a generally accepted dynamical theory of fission, the above experimental findings are discussed in terms of static energy considerations. Triggered from the outset by the structure of the potential energy surface at the saddlepoint, the energy balance at the scission point between the available energy (Q-value) of the reaction and the Coulomb and deformation energy of the ...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

British Library Electronic Table of Contents (United Kingdom)

A fragmented fossil bone incised with the figure of a proboscidean was recently found at Vero Beach, Florida near the location where Late Pleistocene fauna and human bones were recovered from 1913 to 1916. This engraving may represent the oldest and only existing example of Terminal Pleistocene art depicting a proboscidean in the Americas. Because of the uniqueness, rarity, and potential antiquity of this specimen, caution demanded that a variety of tests be used in an attempt to verify its authenticity. The mineralized bone was identified as mammoth, mastodon, or giant sloth. Rare earth element analysis was consistent with the fossil bone being ancient and originating at or near the Old Vero site (8-IR-9). Forensic analysis suggests the markings on the bone are not recent. Optical microsc...

2011-01-01

402

Drivers of Transfusion Decision Making and Quality of the Evidence in Orthopedic Surgery: A Systematic Review of the Literature

British Library Electronic Table of Contents (United Kingdom)

Reasons for variation in transfusion practice in orthopedic surgery are not well understood. This systematic review identified and appraised the quality of the literature in this area to assess the impact of factors associated with the use of allogeneic red blood cell (RBC) transfusion in orthopedic procedures. MEDLINE and EMBASE databases were searched for relevant English language publications. Articles containing a range of MeSH and text terms regarding ''blood transfusion,'' ''predictors,'' and ''multiple logistic regression'' were retrieved. Articles that focused on patients undergoing orthopedic procedures and that met prespecified inclusion criteria were appraised in terms of potential bias and the appropriateness of statistical approach. A total of 3641 citations were retrieved, an...

2011-01-01

403

Darrieus vertical axis wind turbine program overview

Compact Single-Stage Fuel Processor for PEM Fuel Cells. Final report

As a US Department of Energy (DOE) laboratory, Sandia Laboratories is developing Darrieus vertical axis wind turbine (VAWT) technology with the objective of encouraging private industry to produce economically feasible, commercially marketable wind energy systems. The first full cycle of development is essentially complete, and resulting current technology designs have been evaluated for cost-effectiveness. Aerodynamic, structural, and system analyses capabilities have evolved during this cycle to support and evaluate the system designs. This report presents some of the more salient recent developments, first generation costs, current activities, and future plans. Potential design improvements identified in the first development cycle are presented along with their cost benefits.

404

Climatic change and river ice breakup

Based on observations during the steam reforming of ethanol, the authors conclude that carbon was forming in the steam generator due to the thermal decomposition of ethanol. Since ethanol is being thermally decomposed, they were operating the steam generator at too high of a temperature. The thermal degradation of ethanol was confirmed by using a GC with a flame ionization detector. They observed trace amounts of additional hydrocarbons other than methane in the effluent which we assume maybe ethane and ethylene. We identified the operating conditions that allowed us to steam reform ethanol for an acceptable amount of time. These conditions were a steam temperature of 200 C and a wall temperature of 400 C at the center of the reactor. The calculated ratios of CO{sub 2}/CO indicate that we can lower the potential for carbon deposition from the Boudouard further by reducing the pressure.

2000-01-01

405

Climate change and the African baobab (Adansonia digitata L.): the need for better conservation strategies

An overview of climatic factors and impact relative to river ice engineering and science is presented. An explanation of the fundamentals of climatic change is followed by a review of direct and indirect climatic influences that govern river ice breakup and related trends. Known responses of river ice to climatic change and potential future changes to ice breakup processes are described along with the probable ecological and socio-economic consequences of these changes. Changes in engineering approaches to accommodate the present ice regime and predicted changes in climatic variables that affect river ice processes and reduce the vulnerability of infrastructure and ecosystems to climatic change are examined. Future research on the links between river ice and stream ecology is suggested to identify ecological concerns that may result from changes in river ice regimes induced by climatic change. 60 refs., 3 figs.

2003-07-01

406

British Library Electronic Table of Contents (United Kingdom)

Abstract The baobab tree, with more than 300 uses and commercial value in EU and United States, has been identified as one of the most important trees to be conserved and domesticated in Africa. A decline in baobab populations because of changes in climate could have a negative effect on African livelihoods. Therefore, it is important to study the potential future distribution of this species and determine strategies for conservation. We used Maxent, 480 geo-referenced records, present and future climatic and soil layers. Different general circulation models and scenarios were selected. Models were simulated for (i) All records, (ii) East Africa and (iii) West Africa species records. For each combination, the proportion of the present habitat that might remain suitable in the future was de...

2011-01-01

407

Bilateral but not Unilateral Testicular Hypotrophy Predicts for Severe Impairment of Semen Quality in Men with Varicocele Undergoing Infertility Evaluation

British Library Electronic Table of Contents (United Kingdom)

ObjectivesVaricocele is a common cause of infertility, and varicocele-associated testicular hypotrophy has been described as a potential cause of decreased semen quality. We investigated the relationship between testicular hypotrophy and poor semen quality in infertile men with varicoceles. We hypothesized that bilateral hypotrophy is required before the semen quality is severely impaired.MethodsWe retrospectively identified consecutive patients with palpable varicoceles undergoing an infertility evaluation at a single academic center. Each patient was evaluated by the same clinician with history and physical examination. Testicular hypotrophy was defined as a size discrepancy of greater than 3 mL or an absolute size of less than 14 mL. Multivariate logistic regression analysis was used to...

2008-01-01

408

Behind an ambitious megaproject in Asia: The history and implications of the Bakun hydroelectric dam in Borneo

British Library Electronic Table of Contents (United Kingdom)

Using a case-study, inductive, narrative approach, this article explores the history, drivers, benefits, and barriers to the Bakun Hydroelectric Project in East Malaysia. Situated on the island of Borneo, Bakun Dam is a 204m high concrete face, rock filled dam on the Balui River in the Upper Rajang Basin in the rainforests of Sarawak. Bakun Dam and its affiliated infrastructure could be the single largest and most expensive energy project ever undertaken in Southeast Asia. Based on data collected through site visits, original field research in Sarawak, and more than 80 research interviews, the article begins by teasing out the complex history and drivers behind the Bakun project before identifying a set of potential social, political, and economic benefits the project could deliver. It the...

2011-01-01

409

Base flow hydrology and water quality of an Ozarks spring and associated recharge area, southern Missouri, USA

British Library Electronic Table of Contents (United Kingdom)

Human activities in the karst Ozark Plateaus can impact water quality of springs where surface water is rapidly transferred to subsurface conduits. Bennett Spring, in southern Missouri, is the fourth largest spring in the state and supports local tourism activities. Questions regarding poorly functioning on-site wastewater systems (OWS) have raised concerns over the long-term water quality of the spring. This study reports the results of a surface water quality monitoring program in the recharge area where monthly samples were collected at base flow to identify potential pollution sources to the spring. Base flow hydrology of the recharge area was highly variable over the study period, which was drier than normal, causing an incomplete sampling record due to no flow conditions at some site...

2011-01-01

410

Assessment, development and coordination of technology base studies in enhanced heat transfer. Quarterly progress report No. 7, March 1, 1983-May 31, 1983

Applications of nanomaterials in environmental protection; Untersuchung des Einsatzes von Nanomaterialien im Umweltschutz

The major goal of this continuation project is to advance the utilization of heat transfer enhancement techniques in commercial heat exchange equipment. Thirteen heat transfer enhancement techniques have been identified as having potential for improving heat transfer coefficients in full-scale industrial equipment. The following tasks are part of this project, which was originally planned for three years: (1) expansion of technology information base; (2) evaluation of performance of enhanced surfaces and inserts; (3) correlation of data for spirally fluted surfaces; (4) study of structured surfaces in pool boiling; and (5) study of structured surface in spray film evaporation.

1983-08-01

411

A fast model for estimating work-function modifications induced by organic charge-transfer (Sub)monolayers

Following comprehensive research nanomaterials or products which were either still in a re-search/development status or are already available in the marketplace were identified for the water and air sectors. Based on life cycle assessments for two case studies, it was checked how the potential benefits and impacts on the environment for nanotechnology products or processes compare with those for conventional solutions. The first case study deals with the solar treatment of water contami-nated with tetrachloroethylene, comparing nanoscale titanium dioxide (photo-catalysis) and a photo-Fenton process. The second case study on air filtration compares a passenger car cabin-air filter with nanofibres and a conventional filter. (orig.)

2010-06-15

412

A comparison of hepatic in vitro metabolism of T-2 toxin in rats, pigs, chickens, and carp

Calculating work-function modifications for flat-lying conjugated molecules on extended metal surfaces using density functional theory (DFT) is an extremely resource intensive task. This prevents fast screening of new molecules for their potential to optimize metal work functions for good electron or hole injection in organic electronic devices. We present a semi-classical model, which avoids that problem. This is achieved by identifying the dominant processes occurring at the interface between metal and adsorbate in the pinning-regime, which are then parameterizing their description using band-structure DFT calculations for a small training set With the resulting interdependent equations at hand, only simple gas-phase calculations are needed to predict the work-function changes induced by new molecules. The model is tested for ten molecules on three different metal surfaces, where it shows excellent agreement with photoelectron spectroscopy ...

2010-07-01

413

British Library Electronic Table of Contents (United Kingdom)

T-2 toxin, a highly toxic member of the type-A trichothecenes, is produced by various Fusarium moulds that can potentially affect human health. It is strongly cytotoxic for human hematopoietic progenitors. Alimentary toxic aleukia (ATA), a disease typically associated with human, is primarily induced by T-2 toxin. A comparison of the metabolism of T-2 toxin incubated with hepatocytes of rats, piglets, chickens, and the hepatic subcellular fractions (microsomes and cytosol) of piglets, chickens, rats, and carp (common carp and grass carp) was carried out. The activities of the recombinant pig CYP3A29 on the transformation of T-2 and HT-2 toxins were preliminary studied. Metabolites were identified by novel LC/MS-IT-TOF. Qualitative similarities and differences across the species were observ...

2011-01-01

414

Development of a computerized portal verification scheme for pelvic treatment fields

RCRA Facilities Assessment (RFA)---Oak Ridge National Laboratory

Purpose/Objective: At present, treatment verification between portal and reference images is performed based on manually-identified features by radiation oncologist, which is both time-consuming and potentially error-prone. There is a demand for the computerized verification procedure in clinical application. The purpose of this study is to develop a computerized portal verification scheme for pelvic treatment fields. Materials/Methods: The automated verification system involves image acquisition, image feature extraction, feature matching between reference and portal images and quantitative evaluation of patient setup. Electronic portal images with a matrix size of 256 x 256 and 12 bit gray levels were acquired using a liquid matrix electronic portal imaging device. Simulation images were acquired by digitizing simulation films using a TV camera into images with 256 x 256 matrix and 8 bit gray levels. Initially a Canny edge detector is applied ...

1996-09-01

415

Preparation and evaluation of radioiodinated 1-(dialkyl-aminoalkyl)-4-phenylpiperazines as potential brain imaging agents

US Department of Energy (DOE) facilities are required to be in full compliance with all federal and state regulations. In response to this requirement, the Oak Ridge National Laboratory (ORNL) has established a Remedial Action Program (RAP) to provide comprehensive management of areas where past and current research, development, and waste management activities have resulted in residual contamination of facilities or the environment. This report presents the RCRA Facility Assessment (RFA) required to meet the requirements of RCRA Section 3004(u). Included in the RFA are (1) a listing of all sites identified at ORNL that could be considered sources of releases or potential releases; (2) background information on each of these sites, including location, type, size, period of operation, current operational status, and information on observed or potential releases (as required in Section II.A.1 of the RCRA permit); (3) ...

1987-03-01

416

Preparation and evaluation of radioiodinated 1-(dialkyl-aminoalkyl)-4-phenylpiperazines as potential brain imaging agents

The interest in radioiodinated diamines stems from their similarity to /sup 125/I-HIPDM and to the 1-dialkvlamino-acyl-4-phenylpiperazines that the authors have previously examined as potential brain imaging agents. In this study they converted the 1-(dialkylaminoacyl)-4-phenylpiperazines to their corresponding 1-(dialkylaminoacyl) analogs via reduction with diborane in THF. Radioiodination at the no-carrier-added level with Na/sup 125/I and chloramine-T gave the final compounds, after chromatographic separation, in 30-50% yields. The tissue distributions were determined in rats at 0.25, and 4 hrs after an i.v. injection of the radiochemical. The results indicated that all of the agents were readily extracted by the brain (1.5-2.5% ID) with brain to blood ratios >20. The structure-distribution relationships for this series were, however, decidedly different from the aminoacyl compounds in that morpholino-derivatives had better uptake and retention than the ...

1985-05-01

417

Preliminary assessment of condensation behavior for hydrocarbon-vapor expansions which cross the saturation line near the critical point

The interest in radioiodinated diamines stems from their similarity to /sup 125/I-HIPDM and to the 1-dialkvlamino-acyl-4-phenylpiperazines that the authors have previously examined as potential brain imaging agents. In this study they converted the 1-(dialkylaminoacyl)-4-phenylpiperazines to their corresponding 1-(dialkylaminoacyl) analogs via reduction with diborane in THF. Radioiodination at the no-carrier-added level with Na/sup 125/I and chloramine-T gave the final compounds, after chromatographic separation, in 30-50% yields. The tissue distributions were determined in rats at 0.25, and 4 hrs after an i.v. injection of the radiochemical. The results indicated that all of the agents were readily extracted by the brain (1.5-2.5% ID) with brain to blood ratios >20. The structure-distribution relationships for this series were, however, decidedly different from the aminoacyl compounds in that morpholino-derivatives had better uptake and retention than the ...

1985-06-02

418

Nevada Nuclear Waste Storage Investigations: Exploratory Shaft Facility fluids and materials evaluation

Previous analyses of binary cycles for conversion of geothermal energy from moderate temperature resources to electrical energy have shown potential gains in net geofluid effectiveness on the order of 8%, resulting from selection of turbine-expansion processes whose equilibrium states pass through the two-phase region. If condensation occurs, this gain could be reduced or eliminated by the resulting loss in turbine efficiency. Experience with many fluids, however, indicates that vapor supersaturation permits metastable pure-vapor states to exist at temperatures considerably below the saturation temperature at a given pressure; thus, by better understanding the condensation process, and properly structuring the cycle, substantial performance gains may be possible. The purpose of the present study was to assess the probability for attaining this performance gain by estimating the extent of condensation which might be expected during such an expansion of isobutane ...

1983-01-01

419

Methods and findings of the SNR study

The objective of this study was to determine if any fluids or materials used in the Exploratory Shaft Facility (ESF) of Yucca Mountain will make the mountain unsuitable for future construction of a nuclear waste repository. Yucca Mountain, an area on and adjacent to the Nevada Test Site in southern Nevada, USA, is a candidate site for permanent disposal of high-level radioactive waste from commercial nuclear power and defense nuclear activities. To properly characterize Yucca Mountain, it will be necessary to construct an underground test facility, in which in situ site characterization tests can be conducted. The candidate repository horizon at Yucca Mountain, however, could potentially be compromised by fluids and materials used in the site characterization tests. To minimize this possibility, Los Alamos National Laboratory was directed to evaluate the kinds of fluids and materials that will be used and their potential impacts on the site. A ...

1988-11-01

420

Heavy metals in Semarang`s urban streams: Spatial distribution and bioindication using the guppy (Lepistes reticulatus)

A featfinding committee of the German Federal Parliament in July 1980 recommended to perform a ''risk-oriented study'' of the SNR-300, the German 300 MW fast breeder prototype reactor being under construction in Kalkar. The main aim of this study was to allow a comparative safety evaluation between the SNR-300 and a modern PWR, thus to prepare a basis for a political decision on the SNR-300. Methods and main results of the study are presented in this paper. In the first step of the risk analysis six groups of accidents have been identified which may initiate core destruction. These groups comprise all conceivable courses, potentially leading to core destruction. By reliability analyses, expected frequency of each group has been calculated. In the accident analysis potential failure modes of the reactor tank have been investigated. Core destruction may be accompanied by the release of significant amounts of mechanical ...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Estimation of trace elements in some anti-diabetic medicinal plants using PIXE technique

A field survey on cadmium, lead, copper, and zinc was performed in Semarang, the fifth largest city in Indonesia. Water, sediment, and fish samples were collected from 101 grids of 2 x 2 km. The objectives of the study were (1) to identify the spatial distribution of metals in the sediments of the greater Semarang area, (2) to estimate the background concentrations of the metals present in Semarang, (3) to provide a simple tool for deriving standards for metals in the sediment, and (4) to explore the potential use of the guppy (Lebistes reticulatus) as a bioindicator of urban metal pollution. To map the spatial distribution of the metals, concentrations of each metal in sediment were plotted against the corresponding city coordinate. On the basis of these plots, background concentrations of the metals were estimated. A combined pollution index can be derived thereafter by calculating the difference between metals concentrations from a ...

1996-12-31

422

Anaerobic digestion and related best management practices : utilizing life cycle assessment

Trace elemental analysis was carried out in various parts of some anti-diabetic medicinal plants using PIXE technique. A 3 MeV proton beam was used to excite the samples. The elements Cl, K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Br, Rb and Sr were identified and their concentrations were estimated. The results of the present study provide justification for the usage of these medicinal plants in the treatment of diabetes mellitus (DM) since they are found to contain appreciable amounts of the elements K, Ca, Cr, Mn, Cu, and Zn, which are responsible for potentiating insulin action. Our results show that the analyzed medicinal plants can be considered as potential sources for providing a reasonable amount of the required elements other than diet to the patients of DM. Moreover, these results can be used to set new standards for prescribing the dosage of the herbal drugs prepared from these plant materials.

2006-08-15

423

Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene

This paper reported on a life cycle assessment (LCA) study that compared the environmental impacts of business-as-usual manure management with those of a manure management operation incorporating anaerobic digestion with combined heat and power generation. The case study was based on a medium sized dairy farm in northern New York State. The study identified the benefits resulting from the displacement of fossil fuels, and reduction of related emissions. Although anaerobic digestion of dairy manure with energy recovery through biogas combustion is viewed as a positive environmental approach to increase the use of renewable energy, there are potential negative impacts that can counteract the environmental benefits. The negative impacts are associated with emissions of methane and nitrogen species during digestion and after spreading of digester effluent. The environmental impacts and their causes should be evaluated in order to promote best ...

2010-07-01

424

Work plan for the remedial investigation/feasibility study-environmental assessment for the Colonie site, Colonie, New York

Pancreatitis-associated protein (PAP) is a secretory pancreatic protein present in small amounts in normal pancreas and overexpressed during the acute phase of pancreatitis. In this paper, the authors describe the cloning, characterization, and chromosomal mapping of the human PAP gene. The gene spans 2748 bp and contains six exons interrupted by five introns. The gene has a typical promoter containing the sequences TATAAA and CCAAT 28 and 52 bp upstream of the cap site, respectively. They found striking similarities in genomic organization as well as in the promoter sequences between the human and rat PAP genes. The human PAP gene was mapped to chromosome 2p12 using rodent-human hybrid cells and in situ chromosomal hybridization. This localization coincides with that of the reg/lithostathine gene, which encodes a pancreatic secretory protein structurally ...

1994-01-01

425

Study of scalar leptons at the TESLA Photon Collider

This work plan has been prepared to document the scoping and planning process performed by the US Department of Energy (DOE) to support remedial action activities at the Colonie site. The site is located in eastern New York State in the town of Colonie near the city of Albany. Remedial action of the Colonie site is being planned as part of DOE's Formerly Utilized Sites Remedial Action Program. The DOE is responsible for controlling the release of all radioactive and chemical contaminants from the site. Under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), a remedial investigation/feasibility study (RI/FS) must be prepared to support the decision-making process for evaluating remedial action alternatives. This work plan contains a summary of information known about the site as of January 1988, presents a conceptual site model that identifies potential routes of human exposure to site containments, ...

1990-06-01

426

Streamlined Approach for Environmental Restoration (SAFER) Plan for Corrective Action Unit 118: Area 27 Super Kukla Facility, Nevada Test Site, Nevada, Rev. No.: 1

In this thesis, the potential to discover supersymmetric signatures in both e"+e"- and #gamma##gamma# colliders evaluated with a Monte Carlo analysis, is discussed. The analysis was focused on the detection of muons, essentially. First, we study the detection of #mu#"+_R#mu#"-_R in e"-e"+ collisions, whose purpose is the measurement of the #mu#_R mass. It was found that an uncertainty of 0.11 GeV (stat) can be achieved. Under the assumption of the real conditions of the ILC photon collider, a study covering the detection of #mu#"+_R#mu#"-_R and #mu#"+_L#mu#"-_L pairs for #sq root#(s_e_"-_e_"+)=0.5 and 0.6 TeV was performed. According to the simulation, a statistical error for the branching ratios of #mu#"#+-#_L#->##chi#"0_1#mu#"#+-# of 0.98% and #mu#"#+-#_L#->##chi#"0_2#mu#"#+-# of 3.97% can be reached. In order to judge the Monte Carlo results, we have used a technique based on a multidimensional fit to evaluate the impact of the branching ratio measurements ...

427

Possible global environmental impacts of solid waste practices

This Streamlined Approach for Environmental Restoration (SAFER) plan addresses closure for Corrective Action Unit (CAU) 118, Area 27 Super Kukla Facility, identified in the ''Federal Facility Agreement and Consent Order''. Corrective Action Unit 118 consists of one Corrective Action Site (CAS), 27-41-01, located in Area 27 of the Nevada Test Site. Corrective Action Site 27-41-01 consists of the following four structures: (1) Building 5400A, Reactor High Bay; (2) Building 5400, Reactor Building and access tunnel; (3) Building 5410, Mechanical Building; and (4) Wooden Shed, a.k.a. ''Brock House''. This plan provides the methodology for field activities needed to gather the necessary information for closing the CAS. There is sufficient information and process knowledge from historical documentation and site confirmation data collected in 2005 and 2006 to recommend closure of CAU 118 using the ...

2006-09-01

428

Hawaii demand-side management resource assessment. Final report: DSM opportunity report

Pollutants resulting from the management of solid waste have been shown to affect the air, land, oceans, and waterways. In addition, solid wastes have other, more indirect impacts such as reduction in feedstocks of natural resources, because useful materials are disposed of rather than recycled. The objective of this study is to evaluate solid waste management practices that have negative implications on the global environment and develop recommendations for reducing such impacts. Recommendations identifying needed changes are identified that will reduce global impacts of solid waste practices in the future. The scope of this study includes the range of non-hazardous solid wastes produced within our society, including municipal solid waste (MSW) and industrial solid waste (ISW), as well as industry-specific wastes from activities such as construction, demolition, and landclearing. Most solid waste management decisions continue to be made and ...

1994-09-01

429

Electrochemical noise measurements of sustained microbially influenced pitting corrosion in a laboratory flow loop system.

The Hawaii Demand-Side Management Resource Assessment was the fourth of seven projects in the Hawaii Energy Strategy (HES) program. HES was designed by the Department of Business, Economic Development, and Tourism (DBEDT) to produce an integrated energy strategy for the State of Hawaii. The purpose of Project 4 was to develop a comprehensive assessment of Hawaii`s demand-side management (DSM) resources. To meet this objective, the project was divided into two phases. The first phase included development of a DSM technology database and the identification of Hawaii commercial building characteristics through on-site audits. These Phase 1 products were then used in Phase 2 to identify expected energy impacts from DSM measures in typical residential and commercial buildings in Hawaii. The building energy simulation model DOE-2.1E was utilized to identify the DSM energy impacts. More detailed information on the typical buildings and the DOE-2.1E ...

1995-08-01

430

Technology Roadmaps - Electric and plug-in hybrid electric vehicles (EV/PHEV)

Because of the chaotic nature of the corrosion process and the complexity of the electrochemical noise signals that are generated, there is no generally accepted method of measuring and interpreting these signals that allows the consistent detection and identification of sustained localized pitting (SLP) as compared to general corrosion. We have reexamined electrochemical noise analysis (ENA) of localized corrosion using different hardware, signal collection, and signal processing designs than those used in conventional ENA techniques. The new data acquisition system was designed to identify and monitor the progress of SLP by analyzing the power spectral density (PSD) of the trend of the corrosion current noise level (CNL) and potential noise level (PNL). Each CNL and PNL data point was calculated from the root-mean- square value of the ac components of current and potential fluctuation signals, which were measured ...

1999-01-13

431

Preliminary delineation of natural geochemical reactions, Snake River Plain aquifer system, Idaho National Engineering Laboratory and vicinity, Idaho

The mass deployment of electric and plug-in hybrid electric vehicles (EVs and PHEVs) that rely on low greenhouse gas (GHG) emission electricity generation has great potential to significantly reduce the consumption of petroleum and other high CO2-emitting transportation fuels. The vision of the Electric and Plug-in Hybrid (EV/PHEV) Vehicles Roadmap is to achieve by 2050 the widespread adoption and use of EVs and PHEVs, which together represent more than 50% of annual LDV (light duty vehicle) sales worldwide. In addition to establishing a vision, this roadmap sets strategic goals to achieve it, and identifies the steps that need to be taken to accomplish these goals. This roadmap also outlines the roles and collaboration opportunities for different stakeholders and shows how government policy can support the overall achievement of the vision. The strategic goals for attaining the widespread adoption and use of EVs and PHEVs worldwide by 2050 ...

2011-06-15

432

Nuclear Forensics and Attribution for Improved Energy Security: The Use of Taggants in Nuclear Fuel

The U.S. Geological Survey, in cooperation with the U.S. Department of Energy, is conducting a study to determine the natural geochemistry of the Snake River Plain aquifer system at the Idaho National Engineering Laboratory (INEL), Idaho. As part of this study, a group of geochemical reactions that partially control the natural chemistry of ground water at the INEL were identified. Mineralogy of the aquifer matrix was determined using X-ray diffraction and thin-section analysis and theoretical stabilities of the minerals were used to identify potential solid-phase reactants and products of the reactions. The reactants and products that have an important contribution to the natural geochemistry include labradorite, olivine, pyroxene, smectite, calcite, ferric oxyhydroxide, and several silica phases. To further identify the reactions, analyses of 22 representative water samples from sites tapping the ...

1997-05-01

433

NGNP Composites R&D Technical Issues Study

The Global Nuclear Energy Partnership (GNEP), recently announced by DOE Secretary Bodman, poses significant new challenges with regard to securing, safeguarding, monitoring and tracking nuclear materials. In order to reduce the risk of nuclear proliferation, new technologies must be developed to reduce the risk that nuclear material can be diverted from its intended use. Regardless of the specific nature of the fuel cycle, nuclear forensics and attribution will play key roles to ensure the effectiveness of nonproliferation controls and to deter the likelihood of illicit activities. As the leader of the DHS nuclear and radiological pre-detonation attribution program, LLNL is uniquely positioned to play a national leadership role in this effort. Ensuring that individuals or organizations engaged in illicit trafficking are rapidly identified and apprehended following theft or diversion of nuclear material provides a strong deterrent against unlawful activities. Key to ...

434

A Human Reliability Analysis of Pre-Accident Human Errors in the Low Power and Shutdown PSA of the KSNP

This study identifies potential applications and design requirements for ceramic materials (CMs) and ceramic composite materials (CCMs) in the NGNP hightemperature gas-cooled reactor (HTGR) primary circuit. Components anticipated for fabrication from non-graphite CMs and CCMs are identified along with recommended normal and off-normal operating conditions. The evaluation defines required dimensions and material properties of the candidate materials for normal operating conditions (NOC), anticipated transients, abnormal events, and design basis events. The report also identifies additional activities required for codifying the selected materials. The activities include ASTM Standard and ASME Code development and other work to support NRC licensing of the plant. Evaluation of the NGNP baseline design indicates components requiring either CMs or CCMs depend upon the reactor operating temperatures. For a ...

2008-09-01

435

Functional Characterization of Melanocyte Stem Cells in Hair Follicles.

Korea Atomic Energy Research Institute, using the ANS Low Power /Shutdown (LPSD)PRA Standard, evaluated the LPSD PSA model of the KSNP, Younggwang (YGN) Units 5 and 6, and identified the items to be improved. The evaluation results of human reliability analysis (HRA) of the pre-accident human errors in the LPSD PSA model of the KSNP showed that 13 items among 15 items of supporting requirements for those in the ANS PRA Standard were identified as them to be improved. Thus, we newly carried out a HRA for pre-accident human errors in the LPSD PSA model for the KSNP to improve its quality. We considered potential pre-accident human errors for all manual valves and control/instrumentation equipment of the systems modeled in the KSNP LPSD PSA model except reactor protection system/ engineering safety features actuation system. We reviewed 160 manual valves and 56 control/instrumentation equipment. The number of newly ...

2003-04-20

436

The PISA experiment-spallation products identified by Bragg curve spectroscopy

In mice, coat pigmentation requires a stem cell (SC) system in which the survival, proliferation, and differentiation of melanocytes (MCs) are regulated by microenvironments in hair follicles (HFs). In vitro systems are required to analyze the behavior of single melanocyte stem cells (MCSCs) and their potential to form SC systems in vivo. We describe here an experimental system for the isolation, self-renewal, and differentiation of MCSCs, as well as an in vivo reconstitution assay for assessing their potential. Using Dct(tm1(Cre)Bee)/CAG-CAT-GFP mice, we show that, in the presence of stem cell factor and basic fibroblast growth factor and the XB2 feeder cell line, purified MCSCs can undergo clonogenic proliferation, resulting in c-Kit(low) side scatter(low) cells. In culture, these cells maintain their capacity to differentiate and reconstitute an MCSC system in HFs. As these cells are present in the upper part of the HF near the bulge region, ...

2011-07-14

437

A modified immunoblot method to identify substrates of protein kinases

No abstract prepared.

2004-07-01

438

Transcription induces strand-specific mutations at the 5? end of human genes

Full Text Available

439

Trans-Species Polymorphism and Selection in the MHC Class II DRA Genes of Domestic Sheep

A regional analysis of nucleotide substitution rates along human genes and their flanking regions allows us to quantify the effect of mutational mechanisms associated with transcription in germ line...Full Text Available

2008-08-01

440

Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum.

Highly polymorphic genes with central roles in lymphocyte mediated immune surveillance are grouped together in the major histocompatibility complex (MHC) in higher vertebrates. Generally, across vertebrate...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

The role of gene expression in ecological speciation

Rhizobium leguminosarum, biovar viceae, strain RCC1001 contains two glutamine synthetase activities, GSI and GSII. We report here the identification of glnA, the structural gene for GSI. A 2 kb fragment...Full Text Available

1987-03-11

442

The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei.

Ecological speciation is the process by which barriers to gene flow between populations evolve due to adaptive divergence via natural selection. A relatively unexplored area in ecological speciation...Full Text Available

2010-09-01

443

The red-green visual pigment gene region in adrenoleukodystrophy.

We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter...Full Text Available

1994-10-11

444

The opiorphin gene (ProL1) and its homologues function in erectile physiology

Although recent data established that a specific very-long-chain fatty acyl-CoA synthetase is defective in X-linked adrenoleukodystrophy (ALD), the ALD gene is still unidentified. The ALD locus has...Full Text Available

1990-03-01

445

The high Andes, gene flow and a stable hybrid zone shape the genetic structure of a wide-ranging South American parrot

OBJECTIVETo determine if ProL1, a member of the opiorphin family of genes, can modulate erectile physiology, as it encodes a peptide which acts as...Full Text Available

2008-09-01

446

The complexity of gene expression dynamics revealed by permutation entropy

BackgroundWhile the gene flow in some organisms is strongly affected by physical barriers and geographical distance, other highly mobile species are able to overcome such constraints....Full Text Available

447

Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery

BackgroundHigh complexity is considered a hallmark of living systems. Here we investigate the complexity of temporal gene expression patterns using the concept of Permutation Entropy...Full Text Available

448

Species-specific microRNA roles elucidated following astrocyte activation

We investigate a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles...Full Text Available

2010-09-01

449

Sequence variants of the DFNB31 gene among Usher syndrome patients of diverse origin

MicroRNAs (miRNAs) are short non-coding RNAs that play a central role in regulation of gene expression by binding to target genes. Many miRNAs were associated with the function of the central nervous...Full Text Available

2011-05-01

450

SLC25A4 - solute carrier family 25 (mitochondrial carrier; adenine...

PurposeIt has been demonstrated that mutations in deafness, autosomal recessive 31 (DFNB31), the gene encoding whirlin, is responsible for nonsyndromic hearing loss...Full Text Available

451

Role of Calcitonin Gene-Related Peptide in Bone Repair after Cyclic Fatigue Loading

The official name of this gene is "solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4." SLC25A4 is the gene's official symbol. The...

2011-08-13

452

Recent Applications of DNA Microarray Technology to Toxicology and Ecotoxicology

BackgroundCalcitonin gene related peptide (CGRP) is a neuropeptide that is abundant in the sensory neurons which innervate bone. The effects of CGRP on isolated bone cells have been...Full Text Available

453

Rai1 duplication causes physical and behavioral phenotypes in a mouse model of dup(17)(p11.2p11.2)

Gene expression is a unique way of characterizing how cells and organisms adapt to changes in the external environment. The measurements of gene expression levels upon exposure to a chemical can be...Full Text Available

2006-01-01

454

PrognoScan: a new database for meta-analysis of the prognostic value of genes

Genomic disorders are conditions that result from DNA rearrangements, such as deletions or duplications. The identification of the dosage-sensitive gene(s) within the rearranged genomic interval is...Full Text Available

2006-11-01

455

Practical Applications of the Bioinformatics Toolbox for Narrowing Quantitative Trait Loci

BackgroundIn cancer research, the association between a gene and clinical outcome suggests the underlying etiology of the disease and consequently can motivate further studies. The...Full Text Available

456

Polymorphisms in the epidermal growth factor receptor gene and the risk of primary lung cancer: a case-control study

Dissecting the genes involved in complex traits can be confounded by multiple factors, including extensive epistatic interactions among genes, the involvement of epigenetic regulators, and the variable...Full Text Available

2008-12-01

457

Polymorphic Regions Affecting Human Height Also Control Stature in Cattle

BackgroundPolymorphisms in Epidermal Growth Factor Receptor (EGFR) gene may influence EGFR production and/or activity, thereby modulating susceptibility to lung...Full Text Available

458

PEG-conjugated PAMAM Dendrimers Mediate Efficient Intramuscular Gene Expression

Orthologous positions of 55 genes associated with height in four human populations were located on the bovine genome. Single nucleotide polymorphisms close to eight of these genes were significantly...Full Text Available

2011-03-01

459

Overexpression of bacterial ethylene-forming enzyme gene in Trichoderma reesei enhanced the production of ethylene

Generations 5 and 6 (G5 and G6) poly(amidoamine) (PAMAM) dendrimers have been shown to be highly efficient nonviral carriers in in vitro gene delivery. However, their high toxicity...Full Text Available

460

Origins, evolution, and phenotypic impact of new genes

In order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from Pseudomonas...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

On the spontaneous stochastic dynamics of a single gene: complexity of the molecular interplay at the promoter

Ever since the pre-molecular era, the birth of new genes with novel functions has been considered to be a major contributor to adaptive evolutionary innovation. Here, I review the origin and evolution...Full Text Available

2010-10-01

462

Mutation analysis of the ferritin L-chain gene in age-related cataract

BackgroundGene promoters can be in various epigenetic states and undergo interactions with many molecules in a highly transient, probabilistic and combinatorial way, resulting in...Full Text Available

463

Multiple Genes on Chromosome 7 Regulate Dopaminergic Amacrine Cell Number in the Mouse Retina

PurposeTo investigate whether acquired somatic mutations in the iron response element of the ferritin L-chain gene account for the age-related cataract.MethodsThe...Full Text Available

464

Molecular cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

PurposeThe size of neuronal populations is modulated by gene variants that influence cell production and survival, in turn influencing neuronal connectivity, function,...Full Text Available

2009-05-01

465

Leptin receptor gene expression and number in the brain are regulated by leptin level and nutritional status

This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...Full Text Available

1989-11-01

466

Kinetic Complexity of the Global Response to Glucocorticoid Receptor Action

Hormone potency depends on receptor availability, regulated via gene expression and receptor trafficking. To ascertain how central leptin receptors are regulated, the effects of leptin challenge, high-fat...Full Text Available

2009-07-15

467

Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene.

We have characterized the kinetic response of gene targets throughout the murine genome to transcriptional modulation by the glucocorticoid receptor (GR). In contrast to a model in which multiple genes...Full Text Available

2009-04-01

468

Incorporation of dUTP does not mediate mutation of A:T base pairs in Ig genes in vivo

Exploring the possibility of enhancing the properties of baculoviruses as biological control agents of insect pests, we tested the effect of expressing an insect gene (jhe) encoding juvenile hormone...Full Text Available

1992-05-01

469

Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis

Activation-induced cytidine deaminase (AID) protein initiates Ig gene mutation by deaminating cytosines, converting them into uracils. Excision of AID-induced uracils by uracil-N-glycosylase...Full Text Available

2010-12-01

470

Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli.

IntroductionPrevious observations suggest that active systemic juvenile idiopathic arthritis (sJIA) is associated with a prominent erythropoiesis gene-expression signature. The aim...Full Text Available

2010-01-01

471

Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription

A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase....Full Text Available

1990-04-01

472

Heterogeneous mutations in the human lipoprotein lipase gene in patients with familial lipoprotein lipase deficiency.

Cellular hypertrophy is regulated by coordinated pro- and antigrowth machineries. Foxo transcription factors initiate an atrophy-related gene program to counter hypertrophic growth. This study was designed...Full Text Available

2008-09-01

473

Genomic cloning and characterization of a ricin gene from Ricinus communis.

The DNA sequences were determined for the lipoprotein lipase (LPL) gene from five unrelated Japanese patients with familial LPL deficiency. The results demonstrated that all five patients are homozygotes...Full Text Available

1991-12-01

474

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

475

Gene Signature For Predicting Survival Outcome Of Human Hepatocellular Carcinoma (HCC) - Technology Transfer Center

BackgroundDue to the high morbidity and mortality of fulminant hepatitis, early diagnosis followed by early effective treatment is the key for prognosis improvement. So far, little...Full Text Available

476

Fusion expression of pedA gene to obtain biologically active pediocin PA-1 in Escherichia coli *

The National Cancer Institute Laboratory of Human Carcinogenesis is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize a gene signature for prognosis of hepatocellular carcinoma in patients.

477

Full genome gene expression analysis of the heat stress response in Drosophila melanogaster

Two heterologous expression systems using thioredoxin (trxA) as a gene fusion part in Escherichia coli were developed to produce recombinant pediocin PA-1. Pediocin...Full Text Available

2011-01-01

478

Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii?

The availability of full genome sequences has allowed the construction of microarrays, with which screening of the full genome for changes in gene expression is possible. This method can provide a wealth...Full Text Available

2005-10-01

479

Drosophila melanogaster Methoprene-tolerant (Met) gene homologs from three mosquito species: members of PAS transcriptional factor family

Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR....Full Text Available

2009-06-01

480

Dose Optimization for Long-term rAAV-mediated RNA Interference in the Nigrostriatal Projection Neurons

The Methoprene-tolerant (Met) gene in Drosophila melanogaster has been shown to function in juvenile hormone (JH) action. Met...Full Text Available

2007-03-01

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481

Dexamethasone and nitric oxide synthase gene expression in brain.

Short-hairpin RNA (shRNA)–mediated gene knockdown is a powerful tool for targeted gene silencing and an emerging novel therapeutic strategy. Recent publications, however, reported unexpected...Full Text Available

2009-09-01

482

Development of a novel data mining tool to find cis-elements in rice gene promoter regions

Systemic administration of lipopolysaccharide (LPS), which causes endotoxemia and systemic inflammation, has been reported to induce expression of the gene for type II inducible nitric oxide synthase...Full Text Available

1997-03-01

483

Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma

BackgroundInformation on more than 35 000 full-length Oryza sativa cDNAs, together with associated microarray gene expression data collected under various treatment...Full Text Available

484

Comparative genomics of the bacterial genus Listeria: Genome evolution is characterized by limited gene acquisition and limited gene loss

BackgroundPolyethyleneimine (PEI), which can interact with negatively charged DNA through electrostatic interaction to form nanocomplexes, has been widely attempted to use as a gene...Full Text Available

485

Combinatorial Gene Regulation Using Auto-Regulation

BackgroundThe bacterial genus Listeria contains pathogenic and non-pathogenic species, including the pathogens L. monocytogenes and L. ivanovii,...Full Text Available

486

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

As many as 59% of the transcription factors in Escherichia coli regulate the transcription rate of their own genes. This suggests that auto-regulation has one or more important...Full Text Available

2010-06-01

487

Chronic Cocaine-Induced H3 Acetylation and Transcriptional Activation of CaMKII? in the Nucleus Accumbens Is Critical for Motivation for Drug Reinforcement

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

488

Chiropteran types I and II interferon genes inferred from genome sequencing traces by a statistical gene-family assembler

The regulation of gene expression in the brain reward regions is known to contribute to the pathogenesis and persistence of drug addiction. Increasing evidence suggests that the regulation of gene transcription...Full Text Available

2010-03-01

489

Characterization, phylogeny, alternative splicing and expression of Sox30 gene

BackgroundThe rate of emergence of human pathogens is steadily increasing; most of these novel agents originate in wildlife. Bats, remarkably, are the natural reservoirs of many...Full Text Available

490

Characterization of the H(+)-pumping F1F0 ATPase of Vibrio alginolyticus.

BackgroundMembers of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including...Full Text Available

491

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

The F1F0 ATPase of Vibrio alginolyticus was cloned from a chromosomal lambda library. The unc operon, which contains the structural genes for the ATPase, was sequenced and shown to have a gene organization...Full Text Available

1990-12-01

492

Antisense imaging of gene expression in the brain in vivo

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

493

Analysis of the Type IV Fimbrial-Subunit Gene fimA of Xanthomonas hyacinthi: Application in PCR-Mediated Detection of Yellow Disease in Hyacinths

Antisense radiopharmaceuticals could be used to image gene expression in the brain in vivo, should these polar molecules be made transportable through the blood–brain barrier....Full Text Available

2000-12-19

494

A classification-based framework for predicting and analyzing gene regulatory response

A sensitive and specific detection method was developed for Xanthomonas hyacinthi; this method was based on amplification of a subsequence of the type IV fimbrial-subunit gene fimA...Full Text Available

2001-02-01

495

A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides

BackgroundWe have recently introduced a predictive framework for studying gene transcriptional regulation in simpler organisms using a novel supervised learning algorithm called...Full Text Available

496

A Predictive Risk Probability Approach for Microarray Data with Survival as an Endpoint

During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes...Full Text Available

2002-02-01

497

A Brassica rapa Linkage Map of EST-based SNP Markers for Identification of Candidate Genes Controlling Flowering Time and Leaf Morphological Traits

Gene expression profiling has played an important role in cancer risk classification and has shown promising results. Since gene expression profiling often involves determination of a set of...Full Text Available

2008-01-01

498

5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.

For identification of genes responsible for varietal differences in flowering time and leaf morphological traits, we constructed a linkage map of Brassica rapa DNA markers including...Full Text Available

2009-12-01

499