WorldWideScience
 
 
1

Modulation of gene expression in differentiating 3T3 - L1 preadipocytes  

Energy Technology Data Exchange (ETDEWEB)

3T3 - L1 cells can be induced to differentiate by exposing cell culture to a medium containing 3-isobutyl-1-methyl-xanthine together with insulin and dexamethasone. They have observed that oxalomalate (OMA) which inhibits aconitase thus blocking the citric cycle, also favours differentiation to adipocytes leading to fatty acids accumulation and increased synthesis of specific proteins. They compared the effects of 3-isobutyl-1-methyl-xanthine-insulin-dexamethasone and OMA on the steady-state levels of mRNA of c / EBP, H-ferritin and aconitase / IRE-BP. OMA does not affect the steady-state mRNA amount of C / EBP, H-ferritin and aconitase / IRE-BP but the 3-isobutyl-1-methyl-xanthine-insulin-dexamethasone mixture is able to induce a five-fold increase of mRNA levels for aconitase / IRE-BP. This is the first finding correlating iron metabolism to adipocyte differentiation. Further studies will clarify a possible role for the ...

1997-12-31

2

New neutron simulation capabilities provided by the Sandia Pulse Reactor (SPR-III) and the Upgraded Annular Core Pulse Reactor (ACPR)  

Science.gov (United States)

The paper briefly describes the nuclear reactor facilities at Sandia Laboratories which are used for simulating nuclear weapon produced neutron environments. These reactor facilities are used principally in support of continuing R and D programs for the Department of Energy/Office of Military Application (DOE/OMA) in studying the effects of radiation on nuclear weapon systems and components. As such, the reactors are available to DOE and DOD agencies and their contractors responsible for the radiation hardening of advanced nuclear weapon systems. Emphasis is placed upon two new reactor simulation sources; the Sandia Pulse Reactor-III (SPR-III) Facility which enhances the neutron exposure volume capabilities over those presently available with the existing SPR-II Facility, and the Upgraded Annular Core Pulse Reactor (ACPR) Facility which enhances the neutron exposure capabilities over those of the former ACPR Facility.

1978-07-01