harvesting candidate genes: Topics by WorldWideScience.org

Using microarrays to identify positional candidate genes for QTL: the case study of ACTH response in pigs

Full Text Available

2007-12-01

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A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides

BackgroundMicroarray studies can supplement QTL studies by suggesting potential candidate genes in the QTL regions, which by themselves are too large to provide a limited selection...Full Text Available

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Effect of postharvest water deficit stress on gene expression in heads of broccoli (Brassica oleracea var. italica)

During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes...Full Text Available

2002-02-01

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British Library Electronic Table of Contents (United Kingdom)

Harvested plant organs such as heads of broccoli (Brassica oleracea L. var. italica) experience a range of stresses that can lead to premature reduction in quality and eventual senescence. Understanding plant responses to stress may open up novel opportunities to extend postharvest life. One of the first stresses experienced by harvested organs is likely to be water deficit stress since severance of the vascular system halts the normal flux of water into the tissue. For broccoli branchlets with their cut ends held in water, transcriptome analysis based on hybridization of broccoli floret mRNA to a heterologous Arabidopsis microarray revealed that the transcript abundance of 431 genes reliably changed within 48h of harvest. Of these, transcripts of 146 genes increased and 34 genes decreased...

2011-01-01

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Large-scale association analysis of TNF/LTA gene region polymorphisms in type 2 diabetes

A Brassica rapa Linkage Map of EST-based SNP Markers for Identification of Candidate Genes Controlling Flowering Time and Leaf Morphological Traits

BackgroundThe TNF/LTA locus has been a long-standing T2D candidate gene. Several studies have examined association of TNF/LTA...Full Text Available

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Identification of single nucleotide polymorphisms in candidate genes for growth and reproduction in a nonmodel organism; the Atlantic cod, Gadus morhua

For identification of genes responsible for varietal differences in flowering time and leaf morphological traits, we constructed a linkage map of Brassica rapa DNA markers including...Full Text Available

2009-12-01

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Public interest in predictive genetic testing, including direct-to-consumer testing, for susceptibility to major depression: preliminary findings

Recent technological developments have facilitated intensified searches for genetic markers under selection in nonmodel species. Here, we present an approach for the identification of candidate gene variation in nonmodel organisms. We report on the characterization of 82 single nucleotide polymorphisms (SNPs) and on the development of a specific genotyping assay for 30 SNPs in 18 candidate genes for growth and reproduction in Atlantic cod (Gadus morhua). These markers can be used for scanning natural populations for signatures of selection in both contemporary and archived historical samples, for example in retrospective studies assessing the effects of environmental changes, such as increasing temperatures, and selection imposed by high fishing pressure. Furthermore, these gene markers may be of interest to aquaculture, serving as a starting point for linking phenotypic traits ...

2011-01-01

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Large-scale cross-species oncogenomics identifies candidate oncogenes and tumor suppressor genes

The past decade has seen rapid advances in the identification of associations between candidate genes and a range of common multifactorial disorders. This paper evaluates public attitudes towards the...Full Text Available

2010-01-01

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Genetic susceptibility to systemic lupus erythematosus in the genomic era

While genomic alterations identified in human tumors using techniques such as comparative genomic hybridisation (CGH) may be recurrent, they frequently encompass large regions, in some cases...Full Text Available

2010-02-01

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Contrasting Population Structures of the Genes Encoding Ten Leading Vaccine-Candidate Antigens of the Human Malaria Parasite, Plasmodium falciparum

Our understanding of the genetic basis of systemic lupus erythematosus (SLE) has been rapidly advanced using large-scale, case–control, candidate gene studies as well as genome-wide...Full Text Available

2010-12-01

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Computational Challenges in miRNA Target Predictions: To Be or Not to Be a True Target?

The extensive diversity of Plasmodium falciparum antigens is a major obstacle to a broadly effective malaria vaccine but population genetics has rarely been used to guide vaccine design....Full Text Available

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Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea, and their metagenomes

All microRNA (miRNA) target—finder algorithms return lists of candidate target genes. How valid is that output in a biological setting? Transcriptome analysis has proven to be a useful approach...Full Text Available

2009-01-01

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An excess of rare genetic variation in ABCE1 among Yorubans and African-American individuals with HIV-1

BackgroundStructured noncoding RNAs perform many functions that are essential for protein synthesis, RNA processing, and gene regulation. Structured RNAs can be detected by comparative...Full Text Available

2010-01-01

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A Cytogenetic Abnormality and Rare Coding Variants Identify ABCA13 as a Candidate Gene in Schizophrenia, Bipolar Disorder, and Depression

Signatures of natural selection occur throughout the human genome and can be detected at the sequence level. We have re-sequenced ABCE1, a host candidate gene essential for...Full Text Available

2009-12-01

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Clones to replace forest seedlings

Schizophrenia and bipolar disorder are leading causes of morbidity across all populations, with heritability estimates of ∼80% indicating a substantial genetic component. Population genetics...Full Text Available

2009-12-11

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Targeted Capture and Next-Generation Sequencing Identifies C9orf75, Encoding Taperin, as the Mutated Gene in Nonsyndromic Deafness DFNB79

A considerable time can elapse between initial selection of candidate plus trees and harvest of improved seed. The technique showing the greatest promise of shortening this interval is vegetative propagation. Stock plants are grown for two years from seed before the first propagation cycle begins and each plant can be bulked-up 300-500 times over two cycles. An initial stock of 2500 superior Sitka Spruce plants can be multiplied to yield between 300,000 and 750,000 rooted cuttings for forest use within four years from the start of propagation.

1985-01-01

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PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1

Targeted genome capture combined with next-generation sequencing was used to analyze 2.9 Mb of the DFNB79 interval on chromosome 9q34.3, which includes 108 candidate genes. Genomic...Full Text Available

2010-03-12

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LIMD1 is a tumour suppressor gene (TSG) down regulated in ∼80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG...Full Text Available

2011-04-06

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Timber Harvest Plans

Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola

Timber harvesting histories generated by the California Department of Forestry and Fire Protection's Coast-Cascade GIS ... ...

20

British Library Electronic Table of Contents (United Kingdom)

Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzy...

2006-01-01

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Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection.

The retinoic acid receptor beta (Rarb) region of Mmu14 is associated with prion disease incubation time in mouse.

African bovine trypanosomiasis caused by Trypanosoma sp., is a major constraint on cattle productivity in sub-Saharan Africa. Some African Bos taurus breeds are highly tolerant of infection, but the potentially more productive Bos indicus zebu breeds are much more susceptible. Zebu cattle are well adapted for plowing and haulage, and increasing their tolerance of trypanosomiasis could have a major impact on crop cultivation as well as dairy and beef production. We used three strategies to obtain short lists of candidate genes within QTL that were previously shown to regulate response to infection. We analyzed the transcriptomes of trypanotolerant N'Dama and susceptible Boran cattle after infection with Trypanosoma congolense. We sequenced EST libraries from these two breeds to identify polymorphisms that might underlie previously identified quantitative trait loci (QTL), and we assessed QTL regions and candidate loci for ...

2011-05-18

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Central Coast Region Timber Harvesting Plans

In neurodegenerative conditions such as Alzheimer's and prion disease it has been shown that host genetic background can have a significant effect on susceptibility. Indeed, human genome-wide association studies (GWAS) have implicated several candidate genes. Understanding such genetic susceptibility is relevant to risks of developing variant CJD (vCJD) in populations exposed to bovine spongiform encephalopathy (BSE) and understanding mechanisms of neurodegeneration. In mice, aspects of prion disease susceptibility can be modelled by examining the incubation period following experimental inoculation. Quantitative trait linkage studies have already identified multiple candidate genes; however, it is also possible to take an individual candidate gene approach. Rarb and Stmn2 were selected as candidates based on the known association with vCJD. ...

2010-12-06

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Kv1.1 potassium channel deficiency reveals brain-driven cardiac dysfunction as a candidate mechanism for sudden unexplained death in epilepsy(SUDEP)

This data layer consists of polygons representing harvest area boundaries from Timber Harvest Plans approved by the California Department of Forestry ... ...

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Helper T-Cell Epitopes Encoded by the Babesia bigemina rap-1 Gene Family in the Constant and Variant Domains Are Conserved among Parasite Strains

Mice lacking Kv1.1 Shaker-like potassium channels encoded by the Kcna1 gene exhibit severe seizures and die prematurely. The channel is widely expressed in...Full Text Available

2010-04-14

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Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels

Among important candidates for babesial vaccines are apical complex proteins, including rhoptry-associated protein 1 (RAP-1) from Babesia bovis and B. bigemina, which...Full Text Available

1998-04-01

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Timber Harvest Allocation Model

Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived...Full Text Available

2009-01-01

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The Influence of Timber Harvest on the Distribution of Exotic Riparian

Abstract: HARVEST was designed as a strategic research and planning tool, allowing assessment of the spatial pattern consequences of broad timber management ...

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North Quabbin MA Timber Harvesting Study

... the Redwood Creek basin in north coastal California, timber harvest and associated streamside landsliding altered the composition ... ...

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Calculations for harvesting fuel wood with small scale technology integrated with large scale, or two-step harvesting

... this critical information, we analyzed 17 years of timber harvest data gathered for regulatory purposes for a ... ...

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Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.).

Results from this analysis show that the wood-value per hectare is rather the same whether we use the conventional harvester to harvest the pulpwood and timber only, or if we use a combination of a harvester and a chipping system to harvest the small trees for fuel. This is valid if the price of chips is on the same level as pulpwood. The incomes from the wood per hectare reduced with the harvesting costs differ between the two systems. We can see that the revenue per hectare is 34% higher when we harvest pulpwood only (9362 SEK compared to 6702 + 268 SEK). But if we only look at the revenue within the machine-system we can find it is more profitable to use the harvester for processing stems with larger diameter combined with a small scale system for harvesting fuel-wood from the small trees. 7 refs, 7 figs, 2 tabs

1996-11-01

31

Unraveling photosystems. Final technical report

High salinity is one of the main factors limiting cotton growth and productivity. The genes that regulate salt stress in TM-1 upland cotton were monitored using microarray and real-time PCR (RT-PCR) with samples taken from roots. Microarray analysis showed that 1503 probe sets were up-regulated and 1490 probe sets were down-regulated in plants exposed for 3h to 100mM NaCl, and RT-PCR analysis validated 42 relevant/related genes. The distribution of enriched gene ontology terms showed such important processes as the response to water stress and pathways of hormone metabolism and signal transduction were induced by the NaCl treatment. Some key regulatory gene families involved in abiotic and biotic sources of stress such as WRKY, ERF, and JAZ were differentially expressed. Our transcriptome analysis might provide some useful insights into salt-mediated signal transduction pathways in cotton and offer a ...

2011-04-30

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Seasonal variation of defense-related gene expression in leaves from Bois noir affected and recovered grapevines.

This report highlights four main points. (1) A residue substitution in phosphoribulokinase of Synechocystis PCC 6803 renders the mutant light-sensitive. The authors isolated a light-sensitive mutant (BRLS) of the photosynthetic cyanobacterium Synechocystis 6803 that does not survive exposure to bright light; 70% of BRLS cells die upon exposure to light of > 3000 lux for 2 hr. (2) Excitation energy transfer from phycocyanin to chlorophyll in an apcA-defective mutant of Synechocystis sp. PCC 6803. A greenish mutant of the normally bule-green cyanobacterium Synechocystis sp. PC 6803, designated UV6p, was isolated and characterized. UV6p possesses functional photosystems I and II but lacks normal light harvesting phycobilisomes because allophycocyanin is absent and core-specific linker proteins are almost entirely absent. (3) Deletion of the psbG1 gene of the cyanobacterium Synechocystis sp. PCC 6803 leads to the activation of the cryptic psbG2 ...

1997-09-01

33

Association and expression study of synapsin III and schizophrenia

Although Bois noir is one of the main phytoplasma diseases of grapevine, the gene expression and enzyme activities that underlie physiological changes occurring in symptomatic and recovered (with spontaneous or induced symptom remission) plants are mostly unknown. Bois noir symptomatic leaves (September 2006, 2007) and symptomless leaves from infected symptomatic plants (September 2007) of Sangiovese (moderately susceptible) and Chardonnay (highly susceptible) cultivars were collected. Moreover, leaves from infected symptomless plants of both cultivars were harvested in June 2007. Leaves from recovered plants were also collected in the same periods. In recovered plants of both cultivars, class III chitinase and almost every time phenylalanine ammonia-lyase and chalcone synthase expression were increased for all collection periods. In symptomatic leaves of both cultivars, the expressions of the same genes were up-regulated ...

2011-05-31

34

British Library Electronic Table of Contents (United Kingdom)

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter release and in synaptogenesis, suggesting a potential role in several neuropsychiatric diseases. The human SYN3 gene is located on chromosome 22q12-13, a candidate region implicated in previous linkage studies of schizophrenia. However, association studies of SYN3 and schizophrenia have produced inconsistent results. In this study, four SYN3 SNPs (rs133945 (-631 C>G), rs133946 (-196 G>A), rs9862 and rs1056484) were tested in three sets of totally 3759 samples that comprise 655 affected subjects and 626 controls in the Irish Case-Control Study of Schizophrenia (ICCSS), 1350 samples incorporating 273 pedigrees in the Irish Study of High D...

2009-01-01

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Simultaneous harvesting of straw and chaff for energy purposes : influence on bale density, yield, field drying process and combustion characteristics

Isolation of fetal DNA from nucleated erythrocytes in maternal blood

The potential to increase the productivity of fuel straw harvest and transportation was examined. When harvesting straw for energy purposes, only the long fraction is currently collected. However, technological improvements have now rendered it possible to harvest chaff, thus increasing the amount of harvest residues and bale density. The purpose of this study was to determine how harvest yield, bale density, field-drying behaviour and combustion characteristics are affected by the simultaneous harvest of straw and chaff. Field experiments were conducted in 2009 for long- and short-stalked winter wheat crops. Combine harvesting was carried out with 2 different types of combine harvesters. A high-density baler was used to bale the crop residues. Mixing chaff in with the straw swath by combine ...

2010-07-01

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Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. The authors used monoclonal antibody against the transferrin receptor (TIR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, they were successful in detecting the Y chromosomal ...

1990-05-01

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Peru-15, an improved live attenuated oral vaccine candidate for Vibrio cholerae O1.

Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene. The plasmid pEGFP-optS, which contains the codon-optimized SARS-CoV S gene and expresses spike-EGFP fusion protein (S-EGFP) as silencing target and expressing reporter, was transfected with siRNAs into HEK 293T cells. At various time points of posttransfection, the levels of S-EGFP expression and amounts of spike mRNA transcript were detected by fluorescence microscopy, flow cytometry, Western blot, and real-time quantitative PCR, respectively. The results showed that the cells transfected with pEGFP-optS expressed S-EGFP fusion protein at a higher level compared with those transfected with pEGFP-S, which contains wildtype SARS-CoV spike gene ...

2004-11-26

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The genetic basis of salinity tolerance in Arctic charr (Salvelinus alpinus).

Cholera vaccine candidate Peru-15 was derived from a Vibrio cholerae O1 El Tor Inaba strain by deleting the cholera toxin genetic element, introducing the gene encoding cholera toxin B subunit into recA, and screening for nonmotility. In a controlled study, Peru-15 (2 x 10(8) cfu) was administered to 11 volunteers. No vaccinee developed diarrhea, and 10 of 11 had > 4-fold rises in vibriocidal antibody titers. One month later, 5 vaccinees and 5 control volunteers were challenged with wild type V. cholerae O1. Four of 5 controls developed diarrhea (mean, 1.9 L). Two Peru-15 vaccinees developed diarrhea, 1 with < 0.3 L and 1 with approximately 1.0 L; this latter volunteer had not developed a significant vibriocidal immune response to vaccination. Peru-15 shows promise as a single-dose, oral cholera vaccine that is safe, immunogenic, and protective. PMID:7561195

1995-10-01

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Identification of a functional nuclear export signal in the green fluorescent protein asFP499

ABSTRACT: BACKGROUND: The capacity to maintain internal ion homeostasis amidst changing conditions is particularly important for teleost fishes whose reproductive cycle is dependent upon movement from freshwater to seawater. Although the physiology of seawater osmoregulation in mitochondria-rich cells of fish gill epithelium is well understood, less is known about the underlying causes of inter- and intraspecific variation in salinity tolerance. We used a genome-scan approach in Arctic charr (Salvelinus alpinus) to map quantitative trait loci (QTL) correlated with variation in four salinity tolerance performance traits and six body size traits. Comparative genomics approaches allowed us to infer whether allelic variation at candidate gene loci (e.g., ATP1alpha1b, NKCC1, CFTR, and cldn10e) could have underlain observed variation. RESULTS: Combined parental analyses yielded genome-wide significant QTL on linkage groups 8, 14 and 20 for salinity ...

2011-09-21

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Identification of AFAP1L1 as a prognostic marker for spindle cell sarcomas

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

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British Library Electronic Table of Contents (United Kingdom)

Spindle cell sarcomas consist of tumors with different biological features, of which distant metastasis is the most ominous sign for a poor prognosis. However, metastasis is difficult to predict on the basis of current histopathological analyses. We have identified actin filament-associated protein 1-like 1 (AFAP1L1) as a candidate for a metastasis-predicting marker from the gene expression profiles of 65 spindle cell sarcomas. A multivariate analysis determined that AFAP1L1 was an independent factor for predicting the occurrence of distant metastasis (P=0.0001), which was further confirmed in another set of 41 tumors by a quantitative mRNA expression analysis. Immunohistochemical staining using paraffin-embedded tumor tissues revealed that the metastasis-free rate was significantly better...

2011-01-01

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Effects of whole-tree harvesting on nutrient supplies and nutrient cycles in a forest ecosystem: a literature review

Evaluation of chips quality by the analysis of two different harvesting methodologies

Intensive harvesting, whole-tree harvesting, and complete-tree utilization are being incorporated into management plans. Plants require sixteen or more nutrients. To determine impacts of intensive harvesting, research has begun to investigate nutrient losses associated with the removal of harvested material. Research efforts are focusing on quantifying direct nutrient loss from removal of additional biomass and identifying direct nutrient losses associated with whole-tree harvesting operations. An estimated 30-65% increase in biomass removal (from whole-tree harvesting operations as opposed to stem-only harvests) is accompanied by a 100-215% increase in nutrient removal. Whole-tree harvesting of hardwoods doubles the removal of nutrients from stem-only harvests. The significance of direct nutrient ...

1982-01-01

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The effects of chromium VI on the fitness and on the beta-tubulin genes during in vivo development of the nematode Steinernema feltiae.

The Council for Research in Agriculture, Agricultural Engineering Research Unit (CRA-ING) in Rome, Italy has developed an innovative short-rotation forestry (SRF) harvesting system. The system involves a 2 step operation, notably the tree felling and inter-row windrowing performed by a feller windrower equipment; and subsequent chipping performed by a harvester equipped with a pick-up device. The low moisture content of the windrowed trees at harvesting time affects their physical qualities and mechanical strength throughout the chipping operation. The purpose of this study was to analyze the moisture losses of windrowed trees, in relation to the windrow location, on field storage and weather condition. In addition, the study characterized chips quality changes during on field storage by the 2 different harvesting systems. The innovative 2-step system was compared with the traditional 1-step ...

2010-07-01

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an expert system for estimating forest harvesting productivity and cost

The entomopathogenic nematode (EPN), Steinernema feltiae, is a commonly occurring nematode in the soil in Ireland. Consequently, we have conducted investigations as to the utility of this species as a candidate organism for the detection of chromium in Irish soils. These experiments have demonstrated that S. feltiae can survive and reproduce in the presence of high concentrations of chromium VI. It was observed that concentrations as high as 1000 ppm have little effect on the ability of this organism to produce large numbers of progeny. Nematodes were not observed to reproduce above 1800 ppm. However, an increase in development times for the nematode in vivo was noted at concentrations of 400 ppm upwards. This paper also illustrates the effects upon the beta-tubulin genes within nematode populations exposed to chromium VI in vivo. DNA sequencing has shown that elevated levels of variations occur among the population treatments, although these ...

2008-07-18

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The Effect of Different Timber Harvesting Techniques on Plethadontid Salamander Populations.

Sep 1, 2011 ... Title: THINEX - an expert system for estimating forest harvesting productivity and cost. Author: LeDoux, C. B.; Gopalakrishnan, B.; Pabba, R. S. ...

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Nitrogen Fixation, Nodule Development, and Vegetative Regrowth of Alfalfa (Medicago sativa L.) following Harvest ¹

This investigation will explore the effect that current timber extraction techniques have on plethadontid salamander populations in ... group selection, and two-age harvest. Broad scale timber extraction can d...

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Bundling harvester; Nippukorjausharvesteri

Nitrogenase-dependent acetylene reduction, nodule function, and nodule regrowth were studied during vegetative regrowth of harvested (detopped) alfalfa (Medicago sativa L.) seedlings...Full Text Available

1979-07-01

48

Sod peat harvesters - results of time and yield research in summer 1986

The staring point of the project was to design and construct, by taking the silvicultural point of view into account, a harvesting and processing system especially for energy-wood, containing manually driven bundling harvester, automatizing of the harvester, and automatized loading. The equipment forms an ideal method for entrepreneur`s-line harvesting. The target is to apply the system also for owner`s-line harvesting. The profitability of the system promotes the utilization of the system in both cases. The objectives of the project were: to construct a test equipment and prototypes for all the project stages, to carry out terrain and strain tests in order to examine the usability and durability, as well as the capacity of the machine, to test the applicability of the Eko-Log system in simultaneous harvesting of energy and pulp woods, and to start the marketing ...

1996-12-31

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Effects of timber extraction on herb diversity in the Little Tennessee watershed.

More efficient and safe production machines are required for sod peat production. The efficiency and durability of machines is especially important in contract work. Two sod peat harvester types, Pala-14 and Turve-Unsa, were tested by the forest department of the Work Efficiency Associationin summer 1986. Sod peat was collected with these machines at three sod peat production sites. The yield of the Pala-14 harvester was 121 m/sup 3//h for harvesting time, and 56 m/sup 3//h for worksite time, and that of Turve-Unsa was 108 m/sup 3//h and 55 m/sup 3//h, respectively.

1986-01-01

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A harvesting and handling system for sweet sorghum

... study is to collect data in previously harvested timber stands to quantify the differences in herbaceous diversity ... ...

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Design and performance analysis of MAC schemes for Wireless Sensor Networks Powered by Ambient Energy Harvesting

Total and net energies used harvesting 30-cm billets at 8 t/h were 1 and 0.3 kW x h/t, respectively. Harvested crop had 4% leaves, a 70% mass fraction of billets 30-40 cm long, and a 49/sup 0/ angle of repose. A self-unloading forage wagon with beaters and controls metered billets at 1.5 t/h to feed a juice-expression mill.

1983-12-01

52

British Library Electronic Table of Contents (United Kingdom)

Energy consumption is a perennial issue in the design of wireless sensor networks (WSNs) which typically rely on portable sources like batteries for power. Recent advances in ambient energy harvesting technology have made it a potential and promising alternative source of energy for powering WSNs. By using energy harvesters with supercapacitors, WSNs are able to operate perpetually until hardware failure and in places where batteries are hard or impossible to replace. In this paper, we study the performance of different medium access control (MAC) schemes based on CSMA and polling techniques for WSNs which are solely powered by ambient energy harvesting using energy harvesters. We base the study on (i) network throughput (S), which is the rate of sensor data received by the sink, (ii) fair...

2011-01-01

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Rooting of hybrid clones of Populus tremula L. x P. tremuloides Michx. by stem cuttings derived from micropropagated plants

Association of attention-deficit disorder and the dopamine transporter gene

Propagation costs could be cut by replacing part of the micropropagation process with steps involving more traditional techniques. This study explored possibilities for improving existing vegetative propagation techniques for aspen using stem cuttings obtained from micropropagated plants. Vegetative propagation through stem cuttings was studied in 10 micropropagated hybrid aspen clones (Populus tremula L. x P. tremuloides Michx). Cuttings containing one axillary bud were harvested from the same donor plants twice during the growing season: the first harvest in May and the second harvest in July. Rooting percentage was correlated positively with root length, number of roots and height of cutting plant but negatively with length of rooting. The average rooting percentage was 53% in the first harvest and 27% in second harvest. Indole-3-butyric acid treatments (1.2 mM) significantly ...

2001-07-01

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Machinery management data for willow harvest with a bio-baler

Attention-deficit hyperactivity disorder (ADHD) has been shown to be familial and heritable, in previous studies. As with most psychiatric disorders, examination of pedigrees has not revealed a consistent Mendelian mode of transmission. The response of ADHD patients to medications that inhibit the dopamine transporter, including methylphenidate, amphetamine, pemoline, and bupropion, led us to consider the dopamine transporter as a primary candidate gene for ADHD. To avoid effects of population stratification and to avoid the problem of classification of relatives with other psychiatric disorders as affected or unaffected, we used the haplotype-based haplotype relative risk (HHRR) method to test for association between a VNTR polymorphism at the dopamine transporter locus (DAT1) and DSM-III-R-diagnosed ADHD (N = 49) and undifferentiated attention-deficit disorder (UADD) (N = 8) in trios composed of father, mother, and affected offspring. HHRR ...

1995-04-01

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Identification, characterization, and chromosomal localization of the human homolog (hES) of ES/130

Willow harvested in 2-to-3-year rotations has been touted as a reliable source of biomass. A newly designed harvester based on a round baler was developed in 2006. The third generation biobaler was evaluated in 2009 at 2 willow plantations in Quebec. The first site at Godmanchester was a three-year old regrowth with an estimated 52,000 stems/ha of large diameter willow. The biobaler with a flail cutter harvested three plots totalling 4,136 m{sup 2}. Total harvested biomass was 10.36 t DM in 42 bales. The harvest rate averaged 29 bale/h with an average bale size of 1.22 m wide by 1.30 m in diameter. Diesel fuel consumption averaged 0.81 L/bale. Measured losses averaged 11 per cent of the yield. The second site at Saint-Roch-del'Achigan was a two-year old growth with an estimated 49,000 stems/ha of large stem willow. The biobaler harvested seven plots ...

2010-07-01

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Impact of harvesting time on ultimate methane yield of switchgrass grown in eastern Canada

The chicken extracellular matrix glycoprotein ES/130 is necessary for epithelial-mesenchymal transformation in the developing hear and is also expressed in noncardiac chicken tissues such as limb and notochord. We have identified hES, the human homology of chicken ES/130. Fluorescence in situ hybridization analysis (FISH) localizes hES to human chromosome 20p11.2-p12. FISH analyses of individuals with 20p12 deletions and affected by Alagille syndrome exclude hES as a candidate gene for this disorder. Reverse transcriptase-polymerase chain reaction studies reveal that hES is expressed in both fetal and adult human tissues and that hES expression in the left ventricle is increased in the failing adult heart. Further studies will evaluate how hES mutations may relate to congenital human cardiac and skeletal anomalies as well as cardiac remodeling in the adult. 16 refs., 2 figs.

1996-08-01

57

Method for harvesting, cleaning, and treating achenes of guayule (parthenium argentatum gray)

European research in green energy production from crops has resulted in the development of full scale bioreactors that use energy crops as feedstock. Switchgrass has been touted as one of the most promising crops for energy production among several perennial grass species grown under moderate to hot climates. However, few studies have been conducted in colder climate conditions. This study examined the mesophilic methane yield of switchgrass grown under the cooler growing conditions that exist in northeastern North America. In 2007, switchgrass was harvested in late July, August and September and conserved as silage. The regrowth of plots harvested in late July was also harvested in late September as a two-cut strategy. A 30 L small-scale laboratory digester was used to anaerobically digest the switchgrass silage samples. Specific methane yield decreased considerably with advancing plant development, but was similar between ...

2010-07-01

58

Stem cell transplantation for neuroblastoma

Procedures are described to mechanize partially harvesting, cleaning, and pretreatment of guayule achenes. Achenes are harvested with a vacuum insect net and cleaned by a series of screening, threshing, and forced air separations, then treated to overcome seed coat impermeability in a semiautomatic system that presoaks, treats with 0.5% sodium hypochlorite, and rinses. Achenes may be sown immediately or dried for storage. Procedures outlined involve commercially available equipment with a minimum of custom construction and are adaptable to small or large operations. (Refs. 11).

1981-06-01

59

Oregon Timber ... - Global Change Master Directory (GCMD) - NASA

High-risk neuroblastoma is a childhood malignancy with a poor prognosis. Gradual improvements in survival have correlated with therapeutic intensity, and the ability to harvest, process and...Full Text Available

2008-01-01

60

Net energy output from harvesting small-diameter trees using a ...

Summary. Abstract: This theme is for tracking of the BLM timber sale program in the western ... manages for wildlife, recreation, timber harvest, livestock grazing, ...

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61

Factors Influencing the Effectiveness of Swimming Pool Bactericides

Sep 1, 2011 ... Description: What amount of extra energy can be generated after subtracting the total energy consumed to produce the biomass energy? ...

62

Chapter 10: Timber Harvesting and Marketing - Forest Operations Manual

Techniques for culturing, harvesting, and testing bacteria to evaluate bactericidal chemicals for swimming pools are described. Concentrations of 25, 50, and 100 mg of the chlorine stabilizer cyanuric...Full Text Available

1967-05-01

63

G2 checkpoint siRNA screen in irradiated cancer cells: validation of candidate positive hits

Chapter 10 of the manual that describes on-the-ground operations that strengthen the likelihood that our forests will be healthier, more diverse, and ... ...

64

Norwegian Open Research Archives (NORA)

Full Text Available

65

Computational analysis of candidate intron regulatory elements for tissue-specific alternative pre-mRNA splicing

Genetic and physical mapping of the Chediak-Higashi syndrome on chromosome 1q42-43

No abstract prepared.

2001-04-01

66

Association and expression study of synapsin III and schizophrenia.

The Chediak-Higashi syndrome (CHS) is a severe autosomal recessive condition, features of which are partial oculocutaneous albinism, increased susceptibility to infections, deficient natural killer cell activity, and the presence of large intracytoplasmic granulations in various cell types. Similar genetic disorders have been described in other species, including the beige mouse. On the basis of the hypothesis that the murine chromosome 13 region containing the beige locus was homologous to human chromosome 1, we have mapped the CHS locus to a 5-cM interval in chromosome segment 1q42.1-q42.2. The highest LOD score was obtained with the marker D1S235 (Z{sub max} = 5.38; {theta} = 0). Haplotype analysis enabled us to establish D1S2680 and D1S163, respectively, as the telomeric and the centromeric flanking markers. Multipoint linkage analysis confirms the localization of the CHS locus in this interval. Three YAC clones were found to cover the entire region in a contig established by YAC ...

1996-09-01

67

Tuning and controlling gene expression noise in synthetic gene networks

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter release and in synaptogenesis, suggesting a potential role in several neuropsychiatric diseases. The human SYN3 gene is located on chromosome 22q12-13, a candidate region implicated in previous linkage studies of schizophrenia. However, association studies of SYN3 and schizophrenia have produced inconsistent results. In this study, four SYN3 SNPs (rs133945 (-631 C>G), rs133946 (-196 G>A), rs9862 and rs1056484) were tested in three sets of totally 3759 samples that comprise 655 affected subjects and 626 controls in the Irish Case-Control Study of Schizophrenia (ICCSS), 1350 samples incorporating 273 pedigrees in the Irish Study of High Density Schizophrenia Families (ISHDSF), and 564 unrelated schizophrenia patients and 564 healthy individuals in a Chinese ...

2009-09-19

68

Small scale wood chip fuel harvesting and processing

Synthetic gene networks can be used to control gene expression and cellular phenotypes in a variety of applications. In many instances, however, such networks can behave unreliably due to gene expression...Full Text Available

2010-05-01

69

Harvesting operations and energetics of tall grasses for biomass energy production: a case study

The objectives of the project were to demonstrate and evaluate several small-scale methods of harvesting small (200 mm or less) trees and chipping them for fuel, as well as a method of processing such chips for use in dry chip-fired heating appliances. Nine field trials were conducted using various combinations of equipment. The trials consisted of 2 slash reclamation tests, 2 thinning tests, and 5 clear-cutting tests. The tests were conducted on various sites ranging from 0.2 to 1.8 hectares in area. The trees harvested were small; their average stem diameter was 70 mm. Every site was measured before harvest to predict the amount of biomass present. The amount of material obtained from the sites varied from 50% to 80% of the biomass present. Harvesting consisted of chain-saw felling and delimbing, skidding to roadside using winch, and then chipping into a 30 m{sup 3} portable bin. At the processing ...

1985-02-01

70

Harvest of woody crops with a bio-baler in eight different environments in Minnesota

Florida imports about 85% of its energy needs. Among the renewable energy sources available, biomass appears promising in Florida because of a favourable environment for production and the available methods to convert biomass to energy. Optimal production of biomass requires the identification and management of high yielding persistent perennial cultivars. Elephantgrass (Pennisetum purpureum Schum.) and energycane (Saccharum spontaneum L.) are two tall grasses that meet these requirements. To optimize the supply of convertible biomass, suitable methods of harvesting the crop must be available. The purpose of this research was to study the feasibility and energetics of harvesting, drying and baling tall grasses with conventional farm machinery. A Mathews rotary scythe and a New Holland 849 Auto Wrap large round baler were determined to provide a practical harvesting system for baled biomass averaging 15-27 Mg ha [sup -1]. ...

1992-01-01

71

Phytoremediation of Ionic and Methyl Mercury P

The biobaler was originally developed for short-rotation willow plantations, but can currently harvest a wide range of woody crops with a basal diameter up to 150 mm. The biobaler is an alternate approach to harvest woody crops as round bales, generally 1.2 m wide by 1.5 m diameter. In addition to harvesting trees, it can improve management of wild brush, forest understory vegetation and encroaching small trees on abandoned land. It allows easy handling, storage and transportation to sites where the biomass can be used for energy use or other applications. This paper reported on a study that was conducted in the fall of 2009 in which a third generation biobaler was used on 8 different sites across Minnesota, notably Waseca, Madelia, Faribault, Afton, Ogilvie, Hinckley, Aurora and Hibbing. A total of 160 bales were harvested from these sites. The average bale mass was 466 kg and average bale density was ...

2010-07-01

72

Enhancing productivity of Indian basil (Ocimum basilicum L.) through harvest management under rainfed conditions of subtropical north Indian plains

Our long-term goal is to enable highly productive plant species to extract, resist, detoxify, and/or sequester toxic heavy metal pollutants as an environmentally friendly alternative to physical remediation methods. We have focused this phytoremediation research on soil and water-borne ionic and methylmercury. Mercury pollution is a serious world-wide problem affecting the health of human and wild-life populations. Methylmercury, produced by native bacteria at mercury-contaminated wetland sites, is a particularly serious problem due to its extreme toxicity and efficient biomagnification in the food chain. We engineered several plant species (e.g., Arabidopsis, tobacco, canola, yellow poplar, rice) to express the bacterial genes, merB and/or merA, under the control of plant regulatory sequences. These transgenic plants acquired remarkable properties for mercury remediation. (1) Transgenic plants expressing merB (organomercury lyase) extract methylmercury from their ...

1999-06-01

73

British Library Electronic Table of Contents (United Kingdom)

A field experiment was conducted during 2004 and 2005 at the research farm of Central Institute of Medicinal And Aromatic Plants, Lucknow located at 26.5^o N 80.5^o E and 120m above the mean sea level to study the effect of harvesting stage and cutting height on growth, yield and quality of Indian basil. The objective of this study was to increase the essential oil yield of Indian basil without adverse effect on quality by taking two harvests through manipulating harvesting stage and cutting height and to increase the land and rain water utilization efficiency under rain fed condition. The treatments consisted of four stages of first harvest (40, 60, 80 and 100 days after transplanting (DAT)) and three cutting height (0, 7.5 and 15.0cm above ground level). The Indian basil (Ocimum basilicu...

2010-01-01

74

Influence of gamma rays and some pre and post harvest treatments on behavior of some fruits during cold storage

AEC syndrome - Genetics Home Reference

Apricot fruits usually harvested relatively mature but hard enough to withstand-post harvest handling through the marketing chain. These fruits have considerably lower edible quality than tree-ripened fruit. Fruit quality can be improved by delaying harvest least until physiological maturation is completed on the tree (Bonghi et al. 1999) Apricots containing 11% soluble solids concentration, or higher are in high demand by consumers, as fruit have developed considerable taste, aroma and handling for long distance markets. (Kader, 1999). These fruit will be highly perishable, so rapid cold storage to the lowest safe temperature and supplementary treatments (Mc Donald et al, 1999) such as irradiation with the recommend doses (Sillano et al, 1994) or pre-storage heat treatments will be necessary to retard ripening (mainly softening) during 1-2 weeks post harvest life necessary for distribution to distant ...

2003-03-01

75

Effect of dry and wet storage at cool temperatures on the post-harvest performance of Ranunculus asiaticus L. flowers

What genes are related to AEC syndrome? AEC syndrome is caused by mutations in the TP63 gene. This gene provides instructions for making a protein known as p63, which plays an...

2011-10-15

76

British Library Electronic Table of Contents (United Kingdom)

A study was undertaken to assess the effect of different storage temperatures on senescence and postharvest performance of isolated flowers of Ranunculus asiaticus L.. The main aim of the work was to develop a cost-effective storage protocol to reduce the postharvest losses and to bring out the transportation of cut flowers of R. Asiaticus. The flowers were subjected to two different storage treatments, dry storage and wet storage, and their postharvest performance was compared under laboratory conditions. For this purpose, the buds were harvested at 8:00 AM at loose bud stage (Stage II of flower development). The harvested buds were cut to a uniform size of 15 cm and processed for dry or wet storage. For dry storage, the buds were packed after wrapping them in moistened filter papers and ...

2011-01-01

77

Baobab (Adansonia digitata L.) fruit production in communal and conservation land-use types in Southern Africa

British Library Electronic Table of Contents (United Kingdom)

Baobab fruit are harvested and used throughout Africa as an important source of food and are sold to generate income. Commercial use is increasing rapidly as derivatives of the fruit such as baobab seed oil and fruit pulp are being exported to Europe and North America. The cash derived from the sale of fruit support thousands of rural people. This study examines baobab fruit yields in an area being harvested for commercial use. It represents baobab populations and harvesting scenarios typically found in Southern Africa and is the first study in Africa to combine demographic and production data in determining baobab fruit yields. Fruit production was examined across five land-use types (nature reserves, rocky outcrops, plains, fields and villages) and over three consecutive years. Factors a...

2011-01-01

78

A variable step-down conversion ratio switched capacitor DC-DC converter for energy harvesting systems working in intermittent mode

tA Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta

Energy harvesting systems stimulate the development of power management for low power consumption applications. Improving the converter efficiency of power management circuits has become a significant issue in energy harvesting system design. This paper presents a variable step-down conversion ratio switched capacitor (SC) DC-DC converter to advance the converter efficiency of charge on the stored capacitor in a wireless monitoring system of orthopedic implants. The converter is designed to work at 1 MHz switching frequency and achieves 15 to 2 V conversion. Measurement results show that the converter efficiency can reach 42% including all circuit power consumption, which is much higher than previous work. (semiconductor integrated circuits)

2009-12-01

79

Unraveling gene regulatory networks from time-resolved gene expression data -- a measures comparison study

The rice blast resistance (R) gene Pi-ta mediates gene-for-gene resistance against strains of the fungus Magnaporthe grisea that express avirulent...Full Text Available

2000-11-01

80

The ovalbumin gene family: complete sequence and structure of the Y gene.

BackgroundInferring regulatory interactions between genes from transcriptomics time-resolved data, yielding reverse engineered gene regulatory networks, is of paramount importance...Full Text Available

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81

NUCLEIC ACID CONTAINING ELEMENT FOR TRANSFERRING FOREIGN GENE

The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin...Full Text Available

1982-07-24

82

J-STORE (Japan)

Full Text Available

2009-02-27

83

Duplication and Diversification of the Hypoxia-Inducible IGFBP-1 Gene in Zebrafish

Detecting microRNA activity from gene expression data

BackgroundGene duplication is the primary force of new gene evolution. Deciphering whether a pair of duplicated genes has evolved divergent functions is often challenging. The zebrafish...Full Text Available

84

Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family.

BackgroundMicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression by binding to the messenger RNA (mRNA) of protein coding genes. They control gene expression by either...Full Text Available

85

Characterization of Two New Genes, amoR and amoD, in the amo Operon of the Marine Ammonia Oxidizer Nitrosococcus oceani ATCC 19707?

The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase...Full Text Available

1985-07-01

86

A statistical framework for modeling gene expression using chromatin features and application to modENCODE datasets

Molecular analysis of the amo gene cluster in Nitrosococcus oceani revealed that it consists of five genes, instead of the three known genes, amoCAB....Full Text Available

2008-01-01

87

A faster pedigree-based generalized multifactor dimensionality reduction method for detecting gene-gene interactions

We develop a statistical framework to study the relationship between chromatin features and gene expression. This can be used to predict gene expression of protein coding genes, as well as microRNAs....Full Text Available

2011-01-01

88

Functional characterization of a CCCH type zinc-finger protein gene OsZF2 by ectopic overexpression of the gene in rice

We proposed a faster pedigree-based generalized multifactor dimensionality reduction algorithm, called PedG-MDR II (PII), to detect gene-gene interactions underlying complex traits. Inherited...Full Text Available

2011-01-01

89

Senescence Inhibition and Respiration Induced by Growth Retardants and ⁶N-Benzyladenine 1

Full Text Available

2009-03-01

90

Respiration during Postharvest Development of Soursop Fruit, Annona muricata L

Senescence of Grand Rapids leaf lettuce was greatly reduced at 3 storage temperatures by post-harvest treatment with N,N-dimethylaminosuccinamic acid (Alar) and 2-chloroethyltrimethylammonium chloride...Full Text Available

1966-09-01

91

Purification and Characterization of a Membrane-Bound Protease from Chlamydomonas reinhardtii 1

Fruit of soursop, Annona muricata L., showed increased CO₂ production 2 days after harvest, preceding the respiratory increase that coincided with autocatalytic ethylene...Full Text Available

1984-09-01

92

Potential of cattails as an energy source. Final report

In Chlamydomonas reinhardtii y-1, newly synthesized chlorophyll a/b-binding apoproteins are degraded when chlorophylls are not present for assembly of stable light-harvesting...Full Text Available

1992-07-01

93

Post-abscission, pre-dispersal seeds of Digitalis purpurea remain in a developmental state that is not terminated by desiccation ex planta

Research on the feasibility of growing cattails as an energy crop is described. The following topics are included: productivity in natural strands, germination requirements for seed, establishing stands by seeding, rhizome dormancy and development, harvesting and stand establishment, and analysis of canopy structure and radiation profiles in a natural community. (MHR)

1980-01-01

94

Growth velocity and stunting in rural Nepal.

Background and AimsSeed quality may be compromised if seeds are harvested before natural dispersal (shedding). It has been shown previously that slow or delayed drying can increase...Full Text Available

2009-03-01

95

Estimating production rates and operating costs of timber harvesting equipment in the northern Rockies. General technical report

In a community based study, height and weight increments of 441 Nepali children aged 0-6 years were measured before harvest and six months later and compared with centile standards derived from American...Full Text Available

1989-10-01

96

Biology and Effects of Spontaneous Heating in Hay

The report summarizes studies of ground, cable, and aerial logging systems in the Northern Rockies over a 15-year period. It provides nomographs and tables for calculating productivity and a system for comparing energy requirements.

1982-09-01

97

Genetic organization of Bungarus multicinctus protease inhibitor-like proteins.

The negative consequences of baling hay before it is adequately dried are widely known to producers. Frequently, these problems are created by uncooperative weather conditions that prevent forages from drying (rapidly) to moisture levels that allow safe and stable storage of harvested forages. When ...

98

Using modified foragers to harvest short-rotation poplar plantations

The structural organization of the genes encoding Bungarus multicinctus protease inhibitor-like proteins (PILPs), PILP-1, PILP-2 and PILP-3, are reported in this study. Unlike PILP-2 and PILP-3, recombinant PILP-1 exhibited inhibitory activity on trypsin. PILP genes and B chain genes shared identical organization with three exons interrupted by two introns in similar positions. On the contrary, intron 1 of these genes had a similar size, a notable variation with the size of intron 2 was observed. It was found that two regions at the second intron of B1 chain and B2 chain genes were absent in that of PILP genes. Noticeably, intronic insertion in the second intron of B chain genes appeared in the promoter region of PILP-1 gene, but not in that of PILP-2 and PILP-3 genes. Comparative analyses of PILP ...

2008-03-27

99

Spring harvest of corn stover

In Italy, short rotation coppice (SRC) has become very popular in recent years, with over 4000 ha already planted - almost exclusively with clonal poplar. Commercial harvesting operations are dominated by modified foragers, equipped with dedicated SRC headers. At present, contractors use two main header types: German Claas HS-2 and Italian GBE. The former fits smaller foragers with a power below 300 kW, the latter is used on larger harvest units. This study models the performance of modified foragers on a range of short-rotation poplar plantations. Data were collected from 45 operations, covering a total of 118 ha and producing over 4400 green tonnes of wood chips. The average yield of the fields harvested during the trials was about 23 gt ha{sup -1} year{sup -1} (at a moisture content of 59%), and machine productivity ranged from 9 to 70 green tonnes per scheduled machine hour (gt SMH{sup -1}), with an average value of 35 ...

2009-05-15

100

Short rotation coppice harvesting: an evaluation of the Salix Maskiner Bender III

Corn stover is typically left behind in the field after grain harvest. Although part of the stover should remain in the field for soil organic matter renewal and erosion protection, half of the stover could be removed sustainably. This represents about one million t dry matter (DM) of stover per year in the province of Quebec. Stover harvested in the fall is very wet. While there are applications for wet stover, the available markets currently require a dry product. Preliminary measurements have shown that stover left in the field throughout the winter becomes very dry, and a considerable amount would still be harvestable in the spring. In the spring of 2009, corn stover was harvested at 2 sites, each subdivided into 2 parcels. The first parcel was cut and raked in the fall of 2008 (fall parcel), while the second parcel was cut and raked in spring 2009. Fibre from both parcels was baled in the spring ...

2010-07-01

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101

Irradiation as an alternative post harvest treatment

Version III of the Salix Maskiner ``Bender`` coppice harvester was used to successfully harvest 11.2 hectares of short rotation poplar and willow coppice in February 1997. On a wide range of typical UK crop and site conditions, the Swedish harvester was shown to have productivity rates ranging from 4.28 to 7.97 odt/smh. Based on a total system cost of Pound 81.26 per scheduled machine hour, the Bender III is able to produce wood chip for between Pound 10.20 and pound 18.97 per dry tonne. The variation in machine productivity is dependent on crop productivity and working method, emphasising the importance of site design in optimising system efficiency. In willow crops the wood chip produced by the Bender III contained a significant amount of material greater than 75mm in length. The bulk of the chips however, fell into the 5-45mm category with only a very limited amount of fine materials less than 5mm in length. The chips ...

1997-12-31

102

Positrons and antiprotons from inert doublet model dark matter

This current world population has significantly added to the pressures placed upon our finite resources and our resulting ability to feed ourselves. In order to cope with current and future demands, the two established lines of action, that is, reduced population growth and expansion of agricultural production, must be supplemented with the parallel activity of reducing food losses during and after harvest. For developing countries in particular, enormous post-harvest losses result from spillage, contamination, pests and physiological deterioration during storage. Studies in these countries indicate that post-harvest losses are enormous and amount to tens of millions of tons per year valued at billions of dollars. Programs to reduce post-harvest losses, if applied properly, can result in realistic yield increases between 10 and 30%, which can be directly converted into increased consumption for humans. ...

1997-10-27

103

SS 433 as a black hole candidate

We investigate the signatures of antimatter in cosmic rays that would result from annihilations of the scalar dark matter candidate of the Inert Doublet Model. We consider three benchmark candidates, all consistent with the WMAP cosmic abundance and existing direct detection experiments, and confront the predictions of the model with the recent PAMELA, ATIC and HESS data. For a light IDM WIMP candidate, M_{DM} = 10 GeV, we argue that the positron and antiproton fluxes are large, but consistent with expected backgrounds, unless there is an enhancement in the local density of dark matter. For an IDM WIMP candidate with M_{DM} = 70 GeV, the contribution is lower than the expected backgrounds unless there is a large boost factor. However, the candidate is enable to explain the excesses observed by the recent experiments. Finally, for an IDM WIMP candidate with ...

2009-01-01

104

PUTATIVE CSF PROTEIN BIOMARKER CANDIDATES FOR AMNESTIC MILD COGNITIVE IMPAIRMENT

The inverse problem of interpreting the SS 433 optical light curves is solved for a geometrically thick model accretion disk around the compact relativistic object: most likely a black hole.

1985-01-01

105

Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation

The identification of individuals at risk for Alzheimer's disease (AD) is essential for the timely administration of treatment approaches aimed at slowing the onset or progression of the disease....Full Text Available

2010-03-01

106

Metals and kidney autoimmunity.

There is a requirement for a noninvasive technique to monitor stem cell differentiation. Several candidates based on optical spectroscopy are discussed in this review: Fourier transform infrared (FTIR)...Full Text Available

2010-01-01

107

Causality Constrains Higher Curvature Corrections to Gravity

The causes of autoimmune responses leading to human kidney pathology remain unknown. However, environmental agents such as microorganisms and/or xenobiotics are good candidates for that role. Metals,...Full Text Available

1999-10-01

108

A Nearby Old Halo White Dwarf Candidate from the Sloan ...

We show that causality constrains the sign of quartic Riemann corrections to the Einstein-Hilbert action. Our constraint constitutes a restriction on candidate theories of quantum gravity.

2006-01-01

109

Effect of safe environmental pre and post harvest treatments and irradiation on handling of some fruits

... than the age of the universe unless it is an unresolved double degenerate or a product of common-envelope binary star evolution (Fontaine et al. ...

2008-07-01

110

Development of a machine combination for harvesting of small wood and first thinnings; Yhdistelmaekoneen kehittaeminen pienpuun korjuuseen sekae ensiharvennukseen

The present study was carried out during two successive seasons of 2005 and 2006 seasons on"Montakhab El-Kanater"guavas and"Hachiya"persimmons.two different experiments were studied, the first one for pre harvest and second post harvest. Regarding pre harvest experiment,hand or chemical flower thinning by urea or ethrel and date of fruit picking (maturity) were evaluated on both guavas and persimmons.All flower thinning treatments increased fruit set,total yield,average fruit weight and decreased fruit abscission.However,a great effect on fruit quality and chemical compositions were also found with flower thinning treatments. Chemical flower thinning was more effective than hand thinning in improving yield and quality in "Montakhab El-Kanater"guavas and "Hachiya"persimmons. However,early maturation(120 and 150 days for guava and persimmon respect.) produced poor fruit quality.Whereas, medium maturity(130 and 180 days for ...

111

piggyBac Transposon-mediated Long-term Gene Expression in Mice

The objective of the research was to develop a machine combination for harvesting of small wood, which carries out both the harvesting and forest haulage. The development was started in September 1995. The first prototype of the machine is ready. A Lokomo 910 forest tractor was acquired for the tests. The prototype has been mounted on the tractor, and the tests have been started in the beginning of March 1996. The reconstruction of the device will be made after the tests, as well as the description of different working praxis. Time consumption study and the analysis of it will be made after the equipment tests. The device consists of a grapple equipped with a guillotine cutting device mounted on the tractor. The actual felling is made stem by stem in the test phase. The stem can be forwarded directly into the load or it can be left aside, and new stems can be brought beside it and then all the stems can be taken together into load. The ...

1996-12-31

112

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection...Full Text Available

2010-04-01

113

The rapid generation of chimerical genes expanding protein diversity in zebrafish

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

114

The luxS Gene of Streptococcus pyogenes Regulates Expression of Genes That Affect Internalization by Epithelial Cells

BackgroundVariation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin...Full Text Available

115

The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination

The gram-positive pathogen Streptococcus pyogenes was recently reported to possess a homologue of the luxS gene that is responsible for the production of autoinducer...Full Text Available

2003-10-01

116

Specific genetic modifications of domestic animals by gene targeting and animal cloning

Activation-induced deaminase (AID) initiates somatic hypermutation, gene conversion and class switch recombination by deaminating variable and switch region DNA cytidines to uridines. AID is predominantly...Full Text Available

117

Robust consensus clustering for identification of expressed genes linked to malignancy of human colorectal carcinoma

The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available

118

Reverse Engineering of Gene Regulatory Networks: A Comparative Study

Previous studies have been conducted in gene expression profiling to identify groups of genes that characterize the colorectal carcinoma disease. Despite the success of previous attempts to identify...Full Text Available

119

Recurrent miscarriage and variant alleles of mannose binding lectin, tumour necrosis factor and lymphotoxin ? genes

Reverse engineering of gene regulatory networks has been an intensively studied topic in bioinformatics since it constitutes an intermediate step from explorative to causative gene expression...Full Text Available

2009-01-01

120

Rare Homologous Gene Targeting in Histoplasma capsulatum: Disruption of the URA5_Hc Gene by Allelic Replacement

Variant alleles of the mannose binding lectin (MBL) gene are associated with increased susceptibility to infection and polymorphisms of tumour necrosis factor and lymphotoxin alpha genes (TNF, LTA)...Full Text Available

2001-12-01

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121

Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum.

URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5...Full Text Available

1998-10-01

122

Prediction of breast cancer prognosis using gene set statistics provides signature stability and biological context

We have examined the expression and structure of several genes belonging to two classes of vegetative specific genes of the simple eukaryote, Dictyostelium discoideum. In amebae grown on bacteria, deactivation...Full Text Available

1989-12-11

123

PET imaging of heat-inducible suicide gene expression in mice bearing head and neck squamous cell carcinoma xenografts

BackgroundDifferent microarray studies have compiled gene lists for predicting outcomes of a range of treatments and diseases. These have produced gene lists that have little overlap,...Full Text Available

124

Neuronatin: A New Inflammation Gene Expressed on the Aortic Endothelium of Diabetic Mice

The ability to achieve tumor selective expression of therapeutic genes is an area that needs improvement for cancer gene therapy to be successful. One approach to address this is through the...Full Text Available

2009-02-01

125

Mutational analysis of bacteriophage lambda lysis gene S.

OBJECTIVE—Identification of arterial genes and pathways altered in obesity and diabetes.RESEARCH DESIGN AND METHODS—Aortic gene expression profiles of...Full Text Available

2008-10-01

126

GeneSrF and varSelRF: a web-based tool and R package for gene selection and classification using random forest

A plasmid carrying the bacteriophage lambda lysis genes under lac control was subjected to hydroxylamine mutagenesis, and mutations eliminating the host lethality of the S gene were selected. DNA sequence...Full Text Available

1986-09-01

127

Gene-gene and gene-environment interactions: new insights into the prevention, detection and management of coronary artery disease

BackgroundMicroarray data are often used for patient classification and gene selection. An appropriate tool for end users and biomedical researchers should combine user friendliness...Full Text Available

128

Gene-Environment Interactions and Epigenetic Basis of Human Diseases

Despite the recent success of genome-wide association studies (GWASs) in identifying loci consistently associated with coronary artery disease (CAD), a large proportion of the genetic components of...Full Text Available

129

Gene expression analysis of interferon ? in laser capture microdissected cervical epithelium

Most human diseases are related in some way to the loss or gain in gene functions. Regulation of gene expression is a complex process. In addition to genetic mechanisms, epigenetic causes are...Full Text Available

2008-01-01

130

Evolution of DMY, a newly emergent male sex-determination gene of medaka fish.

Optimal sample handling techniques for tissue preparation and storage, RNA extraction and quantification, and target gene detection are crucial for reliable gene expression analysis. Methods...Full Text Available

2008-10-01

131

EDISA: extracting biclusters from multiple time-series of gene expression profiles

The Japanese medaka fish Oryzias latipes has an XX/XY sex-determination system. The Y-linked sex-determination gene DMY is a duplicate of the autosomal gene DMRT1, which encodes a DM-domain-containing...Full Text Available

2004-04-01

132

Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae.

BackgroundCells dynamically adapt their gene expression patterns in response to various stimuli. This response is orchestrated into a number of gene expression modules consisting...Full Text Available

133

Antisense Expression of the CK2 ?-Subunit Gene in Arabidopsis. Effects on Light-Regulated Gene Expression and Plant Growth1

We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen...Full Text Available

1992-11-11

134

Analysis of the bmp Gene Family in Borrelia burgdorferi Sensu Lato

The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because...Full Text Available

1999-03-01

135

An Efficient Fungal RNA-Silencing System Using the DsRed Reporter Gene?

BmpA, BmpB, BmpC, and BmpD are homologous Borrelia burgdorferi lipoproteins of unknown functions, encoded by the bmp genes of paralogous chromosomal gene family 36....Full Text Available

2000-04-01

136

Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.

In filamentous fungi, RNA silencing is an attractive alternative to disruption experiments for the functional analysis of genes. We adapted the gene encoding the autofluorescent DsRed protein as a reporter...Full Text Available

2007-02-01

137

The magnetic resonance and its agro-industry applications; a review

We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expression of nine new and one previously reported (CcnA) sRNAs in strain D39. CcnA is one of five redundant sRNAs reported by Halfmann et al. (A. Halfmann, M. Kovacs, R. Hakenbeck, and R. Bruckner, Mol. Microbiol. 66:110-126, 2007) that are positively controlled by the CiaR response regulator. We characterized 3 of these 14 sRNAs: Spd-sr17 (144 nucleotides [nt]; decreased in stationary phase), Spd-sr37 (80 nt; strongly expressed in all growth phases), and CcnA (93 nt; induced by competence stimulatory peptide). Spd-sr17 and CcnA likely fold into structures containing single-stranded regions between hairpin structures, whereas Spd-sr37 forms a base-paired ...

2010-01-01

138

Management of storage rot in onion through gamma irradiation and chemicals

The electromagnetic properties that present hydrogen and other nucleus of agro-alimentary products, have allowed widely use of magnetic resonance (MR) to study the composition and internal structure of these biological materials in a micro and macroscopic scale, in a nondestructive way. In this paper the physical principles, basic equipment to measure the MR signals and the MR imaging of any biological sample, are explained. It is also shown a review of the main agro-alimentary applications, emphasizing in the use of this principle to examine the internal quality of fruits and vegetables. In these products, the MR has been used to detect and follow the evolution of different factors that determine the internal quality after harvesting, during storage and after post harvesting processes. The main studies that have been conducted with MR in harvested products are about physiological disorders that take place during storage ...

139

takeout, a Novel Drosophila Gene under Circadian Clock Transcriptional Regulation

Pre-harvest sprays of bavistin (50% carbendazim) at 100, 200 and 400 g a.i/ha were ineffective in the control of Aspergillus niger van Tieghem in onion. The combination of gamma irradiation (0.1 kGy) with bavistin dust (0.33g a.i/kg bulbs) was effective to an extent of about 79% in reducing storage losses due to sprouting and rottage. This combination treatment was better than dust treatment alone and highly cost effective. Bavistin, when used as pre-harvest spray, did not leave any residue in the tissues, whereas the compound was seen to move deep into the tissues, when used as post-harvest dust treatment. Higher concentrations of carbendazim residues (2.1-3.4 mg/kg bulb) were detected in the outer scales of the bulbs, as compared to that of inner scales (0.6-1.6 mg/kg), and the residue decreased slightly with passage of storage time. (author). 19 refs., 2 tabs.

140

The early phase change Gene in Maize

We report the identification and characterization of a new Drosophila clock-regulated gene, takeout (to). to is a member of a novel...Full Text Available

2000-09-01

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141

Stochastic gene expression and its consequences

Recessive mutations of the early phase change (epc) gene in maize affect several aspects of plant development. These mutations were identified initially because of...Full Text Available

2002-01-01

142

Ras activation of genes: Mob-1 as a model.

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

143

Mutations in RNA Binding Protein Gene Cause Familial Dilated Cardiomyopathy

The ras oncogenes function by indirectly controlling expression of a subset of yet-undefined genes that are crucial for cell growth and differentiation. In a differential display strategy, numerous...Full Text Available

1994-12-20

144

Mutation frequency in hprt gene in children accidentally exposed to ionizing radiations; Frequencia de mutacao no gene hprt em criancas acidentalmente expostas as radiacoes ionizantes

ObjectivesWe sought to identify a novel gene for dilated cardiomyopathy (DCM).BackgroundDCM is a heritable, genetically...Full Text Available

2009-09-01

145

Molecular cloning and analysis of lymphokines. Volume 13

No abstract prepared

1995-09-01

146

Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male ...

These proceedings collect papers on the subject of lymphokines. Topics include: DNA-cloning of mouse and human lymphokine genes, inteferons, interleukins, gene expression, tumor necrosis factors, and recombinant DNA.

1987-01-01

147

Hormonal Control of Cell Proliferation Requires PASTICCINO Genes

... 041889 Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male Reproducti...

148

Gpnmb is a Melanoblast-Expressed, MITF-Dependent Gene

PASTICCINO (PAS) genes are required for coordinated cell division and differentiation during plant development. In loss-of-function pas mutants,...Full Text Available

2003-07-01

149

Genomics of human longevity

SUMMARYExpression profile analysis clusters Gpnmb with known pigment genes, Tyrp1, Dct, and Si. During development,...Full Text Available

2009-02-01

150

Gene therapy for ocular diseases

In animal models, single-gene mutations in genes involved in insulin/IGF and target of rapamycin signalling pathways extend lifespan to a considerable extent. The genetic, genomic and epigenetic influences...Full Text Available

2011-01-12

151

Cloning of the neurodegeneration gene drop-dead and characterization of additional phenotypes of its mutation

The eye is an easily accessible, highly compartmentalised and immune-privileged organ that offers unique advantages as a gene therapy target. Significant advancements have been made in understanding...Full Text Available

2011-05-01

152

Cis-regulatory mutations in human disease

Mutations in the Drosophila gene drop-dead (drd) result in early adult lethality and neurodegeneration, but the molecular identity of the drd...Full Text Available

2008-01-01

153

Analysis of gene TP53 in samples of ocular epithelium in patients with senile cataract; Analise do gene TP53 em amostras de epitelio ocular obtidas de pacientes com catarata senil

Cis-acting regulatory sequences are required for the proper temporal and spatial control of gene expression. Variation in gene expression is highly heritable and a significant determinant...Full Text Available

2009-07-01

154

Post harvest improvement of zeaxanthin content of vegetables

No abstract prepared.

2000-09-01

155

British Library Electronic Table of Contents (United Kingdom)

Zeaxanthin is a carotenoid produced by plants and has been associated with protection of the photosynthetic machinery under light stress and, together with lutein, in protection of the central retina of the eye. Zeaxanthin levels in blood plasma have been negatively correlated to the development of AMD (age-related macular degeneration) (Gale et al., 2003). Under normal conditions, plants have a low content of zeaxanthin. The aim of this study was to increase the zeaxanthin content in green vegetables by post harvest treatments. Efficient conditions for activation of the endogenous enzyme system generating zeaxanthin was established and included incubation at low pH (2.5-5.5), with the membrane permeable acetic acid/acetate buffer at room temperature or above for 30min or more. Typically m...

2010-01-01

156

Influence of gamma-irradiation, growth retardants and coatings on the shelf life of winter guava fruits (Psidium guajava L.)

British Library Electronic Table of Contents (United Kingdom)

Experiment was conducted to study the effect of gamma irradiation, growth retardants and coatings (coconut oil, mustard oil and liquid paraffin) on shelf life of winter guava fruits during storage. The results revealed that the superiority of coconut oil coating over other post harvest treatments. Physiological loss in weight (7.1%), marketable fruits retained over control (86.7%), total soluble solid (16.1%), ascorbic acid (195 mg/100 g pulp) and total sugar (10%) of fruit were positively influenced by coconut oil coating up to 12 days of storage. The treatment was found significantly effective in increasing the post harvest life of fruits for 12 days over control without adversely affecting the fruit quality. Coconut oil coating gave highest consumer acceptability while, maintaining suff...

2010-01-01

157

Anderson introduces a new biomass baler

T Cell Responses to the RTS,S/AS01_E and RTS,S/AS02_D Malaria Candidate Vaccines Administered According to Different Schedules to Ghanaian Children

Canadian-based Anderson Group Company has developed an innovative round baler for harvesting a large variety of woody biomass. The baler was initially developed in 2005 in collaboration with the University Laval and Agriculture and Agri-Food Canada. The third generation BIOBALER{sup TM} is currently built, engineered and commercialized by Anderson. It can produce up to 40 bales/hr in short rotations woody crops such as willow and hybrid poplar. The unit can harvest brushes up to 125 mm in diameter. A standard tractor can pull the BIOBALER in fallow or abandoned land, under power transmission lines, and between planted trees. The patented BIOBALER includes a mulcher head attachment, a choice of long or short swivel tongue, a fixed chamber and an undercarriage frame.

2010-07-01

158

Preliminary Assessment of the Safety and Immunogenicity of a New CTX?-Negative, Hemagglutinin/Protease-Defective El Tor Strain as a Cholera Vaccine Candidate

BackgroundThe Plasmodium falciparum pre-erythrocytic stage candidate vaccine RTS,S is being developed for protection of young children against...Full Text Available

159

A Randomized Trial Assessing the Safety and Immunogenicity of AS01 and AS02 Adjuvanted RTS,S Malaria Vaccine Candidates in Children in Gabon

Vibrio cholerae 638 (El Tor, Ogawa), a new CTXΦ-negative hemagglutinin/protease-defective strain that is a cholera vaccine candidate, was examined for safety and immunogenicity...Full Text Available

1999-02-01

160

Working paper on candidate mining sites for surface mining of western oil shales

BackgroundThe malaria vaccine candidate antigen RTS,S includes parts of the pre-erythrocytic stage circumsporozoite protein fused to the Hepatitis B surface antigen. Two Adjuvant...Full Text Available

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161

The DG environment project ''Dioxin emissions in candidate countries''. Scope, approach and first results

This report describes the selection of candidate surface mining sites for the development of oil shale resources in the western United States. The purpose of this study is to input the various scenarios into an economic mine model developed for underground oil shale mines in an earlier project. Site selection was a three-phase effort. Phase I consisted of a thorough review of available resource data, which resulted in a definition of surface mineable areas. Phase II was a detailed investigation of the regions identified in the initial phase. The end result of this task was a list of potential sites having favorable overburden, grade, shale thickness, and topography values. Phase III identified those sites from which a wide range of site specific parameters can be obtained for mine design and model testing purposes. This report describes the procedures used to obtain the candidate mining scenarios. Section 2.0 discusses the methodology used to ...

1981-09-02

162

Synthesis and cloning of the genes of antisense peptides of human calcitonin and miniproinsulin

The European Commission recently has adopted a new European Strategy for Environment and Health focusing on the growing concern over the adverse health effects caused by environmental pollution. Based on the results of various studies carried out in recent years polychlorinated dioxins and furans (PCDD/Fs) and PCBs are playing an important role in this strategy. In view of the enlargement of the European Union (EU) to include Candidate Countries (CCs) the Commission stressed the need to involve the Candidate Countries from an early stage on in the development of such a strategy. Thus two studies have been commissioned which are particularly directed to provide a) an overview on environmental levels and human exposure of dioxins and PCBs and b) an harmonised PCDD/F emission inventory for the target matrices air, water and land The latter study, entitled ''Dioxin Emissions in Candidate ...

2004-09-15

163

SIMPLE METHOD FOR SIMULTANEOUSLY INHIBITING FUNCTION OF MULTIPLE GENE IN NEMATODE

With the aim of an experimental check on the validity of the theory of molecular recognition, the authors have carried out the chemical-enzymatic synthesis and cloning of the gene of human calcitonin and also of the genes of antisense polypeptides to human calcitonin and miniproinsulin. It has been shown that recombinant plasmids obtained on the basis of these synthetic genes are capable of ensuring the biosynthesis of the given polypeptides in E. coli cells as hybrid proteins with the IgG-binding domain of staphylococcal protein A.

1994-07-20

164

J-STORE (Japan)

Full Text Available

2009-10-09

165

Promoter Structure Which Affects on the Expression of Yeast MGMT Gene

Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase.

Full Text Available

1997-01-01

166

Molecular Cloning and Characterization of the Yew Gene Encoding Squalene Synthase from Taxus cuspidata

A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named...Full Text Available

1991-10-01

167

Investigation of the Association between Normal-tension Glaucoma and Single Nucleotide Polymorphisms in Natriuretic Peptide Gene

Full Text Available

2007-09-01

168

Immunohistochemical expression of p63 in human prenatal tooth primordia

Full Text Available

169

Hybridization with synthetic oligonucleotides

development, histochemical, human, malformation, p63 gene, tooth

2005-01-01

170

Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or heteromeric artificial miRNA targeting AP1 and CAL genes

Procedures are described for the use of synthetic oligonucleotides for Southern blot experiments and gene bank screening, and the effect of various mismatches on the efficiency of hybridization is demonstrated. The following topics are discussed: sensitivity vs. specificity, hybridization of a 12-mer to the lambda endolysin gene; hybridization of oligonucleotide probes to the E. coli lac operator; hybridization of synthetic probes to the CYC1 gene of yeast; and cloning eucaryotic genes. (HLW)

1978-01-01

171

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.

Gene silencing is a useful technique for elucidating biological function of genes by knocking down their expression. A recently developed artificial microRNAs (amiRNAs) exploits an endogenous...Full Text Available

2009-03-01

172

GENE EXPRESSION VECTOR USING INSULATOR ORIGINATED FROM SEA URCHIN

We have isolated the human prointerleukin 1 (proIL-1) beta gene from leukocyte and fetal liver libraries. The nucleotide sequence and its gene organization reveals that the proIL-1 beta gene is composed...Full Text Available

1986-10-24

173

J-STORE (Japan)

Full Text Available

2010-03-05

174

Delineation of the ADULT syndrome phenotype due to arginine 298 mutations of the p63 gene

DNA, Genes and Chromosomes

Udgivelsesdato: 2006/8

2006-01-01

175

Construction of the Transgenic Drosophila melanogaster Expressing a Human Megsin Gene.

Today you will learn about the parts of DNA and what DNA, genes and chromosomes are. Today you will learn what DNA, genes and chromosomes are and the parts of the DNA molecule. Look at all of the websites, take whatever notes you need to. At the end of the assignment, be able to describle DNA, the parts of DNA, genes and chromosomes. Covers Biology Core Curriculum, ...

2007-11-07

176

Construction and Verification of Useful Vectors for Ectopic Expression and Suppression of Plant Genes.

Full Text Available

2007-05-01

177

Cell Cycle-dependent Expression of Chitin Synthase Genes in Aspergillus nidulans

Full Text Available

2009-06-01

178

Analysis of the Relationship between MHC-DRB1 Gene Polymorphism and Hydatidosis in Kazakh Sheep

Full Text Available

2001-03-01

179

S1 nuclease analysis of #alpha#-globin gene expression in preleukemic patients with acquired hemoglobin H disease after transfer to mouse erythroleukemia cells

Full Text Available

2010-09-01

180

Wool-waste as organic nutrient source for container-grown plants

The loss of #alpha#-globin gene transcriptional activity rarely occurs as an acquired abnormality during the evolution of myeloproliferative disease or preleukemia. To test whether the mutation responsible for the loss of #alpha#-globin gene expression (hemoglobin H disease) in these patients is linked with the #alpha#-globin genes on chromosome 16, the authors transferred chromosome 16 from preleukemic patients with acquired hemoglobin H disease to mouse erythroleukemia cells and measured the transcriptional activity of the human #alpha#-globin genes. After transfer to mouse erythroleukemia cells, the expression of human #alpha#-globin genes from the peripheral blood or marrow cells of preleukemic patients with acquired hemoglobin H disease was similar to that of human #alpha#-globin genes transferred to mouse erythroleukemia cells from normal donors. These ...

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181

Vitrification inches towards a solution

A container experiment was conducted to test the hypothesis that uncomposted wool wastes could be used as nutrient source and growth medium constituent for container-grown plants. The treatments were: (1) rate of wool-waste application (0 or unamended control, 20, 40, 80, and 120 g of wool per 8-in. pot), (2) growth medium constituents [(2.1) wool plus perlite, (2.2) wool plus peat, and (2.3) wool plus peat plus perlite], and (3) plant species (basil and Swiss chard). A single addition of 20, 40, 80, or 120 g of wool-waste to Swiss chard (Beta vulgaris L.) and basil (Ocimum basilicum L.) in pots with growth medium provided four harvests of Swiss chard and five harvests of basil. Total basil yield from the five harvests was 1.6-5 times greater than the total yield from the unamended control, while total Swiss chard yield from the four harvests was 2-5 times greater relative to the respective unamended ...

2009-07-01

182

Regulation of the mRNA for monocyte-derived neutrophil-activating peptide in differentiating HL60 promyelocytes.

A review is presented of the work being carried out around the world on the vitrification of high-level waste. A description is given of the French AVM process, West Germany's research on the Vera, Fips and Pamela processes and the United Kingdom Harvest process. Mention is also made of the Lotes process being developed by Belguim, and of the inactive vitrification plant in Italy, Ivet 1. (U.K.).

183

Evaluation of High Solids Alkaline Pretreatment of Rice Straw

A cDNA library was constructed from HL60 human promyelocyte poly(A)+ RNA harvested 3 h after induction of macrophage differentiation with 12-O-tetradecanoyl phorbol-13-acetate in the presence of cycloheximide....Full Text Available

1989-05-01

184

Fresh-harvested, air-dried rice straw was pretreated at a water content of 5 g H₂O/g straw using sodium hydroxide (NaOH) and compared to pretreatment at 10 g H₂O/g...Full Text Available

2010-11-01

185

Biomass thesaurus

Search for Neutron-Antineutron Oscillations Using Multiprong Events in Soudan 2

This thesaurus contains the vocabulary used to index the Biomass Database maintained by the IEA Biomass Conversion Technical Information Service. The database, which is used to produce Biomass Abstracts, contains indexed and abstracted references to the published literature relating to biomass energy including cultivations, harvesting, materials handling, conversion techniques, environmental aspects and economics.

1984-10-01

186

Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene

We have searched for neutron-antineutron oscillations using the 5.56 fiducial kiloton-year exposure of the Soudan 2 iron tracking calorimeter. We require candidate n-nbar occurrences to have .GE. 4 prongs (tracks and showers) and to have kinematics compatible with nbar-N annihilation within a nucleus. We observe five candidate events, with an estimated background from atmospheric neutrino and cosmic ray induced events of 4.5 \\pm 1.2 events. Previous experiments with smaller exposures observed no candidates, with estimated background rates similar to this experiment. We set a lifetime lower limit for oscillation time in iron: T_A(Fe) > 7.2x10^{31} years. The corresponding lower limit for oscillation of free neutrons is \\tau_{n-nbar} > 1.3x10^8 seconds.

2002-01-01

187

Vital Redundancy: The Case for Organic Airlift Capability in Air ...

Pancreatitis-associated protein (PAP) is a secretory pancreatic protein present in small amounts in normal pancreas and overexpressed during the acute phase of pancreatitis. In this paper, the authors describe the cloning, characterization, and chromosomal mapping of the human PAP gene. The gene spans 2748 bp and contains six exons interrupted by five introns. The gene has a typical promoter containing the sequences TATAAA and CCAAT 28 and 52 bp upstream of the cap site, respectively. They found striking similarities in genomic organization as well as in the promoter sequences between the human and rat PAP genes. The human PAP gene was mapped to chromosome 2p12 using rodent-human hybrid cells and in situ chromosomal hybridization. This localization coincides with that of the reg/lithostathine gene, which encodes a pancreatic secretory protein structurally ...

1994-01-01

188

Tat-Neutralizing Antibodies in Vaccinated Macaques

... AFSOC could look at several aircraft as candidates, including the C-27J, C-130J, and KC-767. ... C-130 aircraft (except C-130J) are normally limited ...

2009-04-01

189

Single Molecule Source Reagents for CVD of Beta Silicon Carbide.

The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade...Full Text Available

2003-03-01

190

Scalp Angiosarcoma Remission with Bevacizumab and Radiotherapy without Surgery: A Case Report and Review of the Literature

Beta silicon carbide is an excellent candidate semiconductor material for demanding applications in high power and high temperature electronic devices due to its high breakdown voltage, relatively large band gap, high thermal conductivity and high melting...

1991-01-01

191

Nurses' knowledge, attitudes, and beliefs regarding organ and tissue donation and transplantation.

Angiosarcoma (AS) is a rare and aggressive vascular neoplasm with very poor prognosis. Patients with extensive cutaneous AS who are not surgical candidates have very limited options since there is no...Full Text Available

2011-01-01

192

Nuclear structure properties for gamma-ray lasers

The acute shortage of human organs and tissues for transplantation has been attributed in part to health professionals, including nurses, for their reluctance to recognize and refer suitable candidates...Full Text Available

1991-03-01

193

Nuclear structure properties for gamma-ray lasers

We summarize some initial results in our investigation of the nuclear physics issues of gamma-ray lasers. We describe what is known thus far from existing experimental data and illustrate how theoretical models may be employed for systematic searches of candidate nuclei.

1986-08-15

194

Novel Approach for Differential Diagnosis of HIV Infections in the Face of Vaccine-Generated Antibodies

We summarize some initial results in our investigation of the nuclear physics issues of gamma-ray lasers. We describe what is known thus far from existing experimental data and illustrate how theoretical models may be employed for systematic searches of candidate nuclei.

1985-01-01

195

Inhibition of Osteoclastogenesis by Mechanically Loaded Osteocytes: Involvement of MEPE

SummaryBecause increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine-from virus-induced antibodies. Most of the currently...Full Text Available

2006-11-01

196

Identification of a stem cell candidate in the normal human prostate gland

In regions of high bone loading, the mechanoresponsive osteocytes inhibit osteoclastic bone resorption by producing signaling molecules. One possible candidate is matrix extracellular phosphoglycoprotein...Full Text Available

2010-11-01

197

Identification of Cardiac Myosin-binding Protein C as a Candidate Biomarker of Myocardial Infarction by Proteomics Analysis*

Stem cells of the human prostate gland have not yet been identified utilizing a structural biomarker. We have discovered a new prostatic epithelial cell phenotype-expressing cytokeratin 6a (Ck6a+...Full Text Available

2005-03-01

198

How and when can one identify hadronic molecules in the baryon spectrum

Acute myocardial infarction (AMI) is a common cause of death for which effective treatments are available provided that diagnosis is rapid. The current diagnostic gold standards are circulating cardiac...Full Text Available

2009-12-01

199

Glucosinolate breakdown products as insect fumigants and their effect on carbon dioxide emission of insects

A method to identify hadronic molecules in the particle spectrum is reviewed and the conditions for its applicability discussed. Special emphasis is put on the discussion of molecule candidates in the baryon spectrum. (orig.)

2008-03-15

200

Evaluation of Two Homologous Proline-Rich Proteins of Coccidioides posadasii as Candidate Vaccines against Coccidioidomycosis?

BackgroundGlucosinolate breakdown products are volatile, therefore good candidates for insect fumigants. However, although they are insecticidal, the mode of action of such natural...Full Text Available

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201

Earth and Environment: Person detail

Evaluation of the protective efficacy of recombinant T-cell-reactive proteins of Coccidioides posadasii in a murine model of coccidioidomycosis has led to the discovery of potential...Full Text Available

2007-12-01

202

Wastenet

...October 2006 - present: University of Leeds, UK PhD Candidate in Geochemistry Thesis Title: Magnetite formation in reducing natural environments Supervisors: Dr Liane G. Benning ...Germany Bachelor of Science in Geosciences and Astrophysics Project Details Project Title: \\

203

Development of engineered structural barriers for nuclear-waste packages

Cold dark matter annihilations: A source of gamma rays and antiprotons

The development of structural barriers for nuclear waste packages involves selection of candidate materials, their screening by mechanical and corrosion testing, rigorous accelerated testing, and evaluation and comparison with other package elements. This document presents results from work conducted on titanium and ferrous alloys.

1981-09-01

204

Candidate salivary biomarkers associated with alveolar bone loss: cross-sectional and in vitro studies

Massive particle candidates for dark matter provide a potentially observable signature by virtue of their annihilations in the galactic halo at a known but model-dependent rate. Possible signatures are described, including cosmic ray antiproton and gamma-ray production. (orig.).

1989-08-01

205

A Response Regulator That Represses Transcription of Several Virulence Operons in the Group A Streptococcus

This cross-sectional study evaluated the association between radiographic evidence of alveolar bone loss and the concentration of host-derived bone resorptive factors (interleukin-1 beta, tumor...Full Text Available

2007-03-01

206

Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

A search for homologs of the Bacillus subtilis PhoP response regulator in the group A streptococcus (GAS) genome revealed three good candidates. Inactivation of one of these, recently...Full Text Available

1999-06-01

207

British Library Electronic Table of Contents (United Kingdom)

To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-...

2011-01-01

208

Nondestructive Detection Techniques of Garter Springs from CANDU Reactors

LUCIFER, a potentially background-free approach to the search for neutrinoless double beta decay

The design and material characteristics of garter spring were summarized and Nondestructive detection techniques of garter spring were also described. In particular, Eddy current testing of loose type garter spring was used in Wolsung unit 1 and was described in detail. The inspection technique of tight type garter spring has not been established and all candidated techniques were investigated in order to choose the possible detection technique. Candidated nondestructive techniques including RFEC, PEC, Magnetic technique using GMR sensor, AE, Guided Wave technique, and high frequency ultrasonic technique, are summarized for evaluating the detectability of tight garter spring.

2004-04-15

209

British Library Electronic Table of Contents (United Kingdom)

LUCIFER (Low-background Underground Cryogenic Installation For Elusive Rates) is a new project for the study of neutrinoless Double Beta Decay, based on the technology of scintillating bolometers. These devices promise a very efficient rejection of the alpha background, opening the way to a virtual background-free experiment if candidates with a transition energy higher than 2615 keV are investigated. The baseline candidate for LUCIFER is 82Se. This isotope will be embedded in ZnSe crystals grown with enriched selenium and operated as scintillating bolometers in a low-radioactivity underground dilution refrigerator. In this paper, the LUCIFER concept will be introduced. The sensitivity and the very promising prospects related to this project will be discussed.

2011-01-01

210

European Space Power Conference

Advances in molecular biology: impact on rotavirus vaccine development.

To support the Space Exploration Initiative (SEI), a study was performed to investigate power system alternatives for the rover vehicles and servicers that were subsequently generated for each of these rovers and servicers, candidate power sources incorporating various power generation and energy storage technologies were identified. The technologies were those believed most appropriate to the SEI missions, and included solar, electrochemical, and isotope systems. The candidates were characterized with respect to system mass, deployed area, and volume. For each of the missions a preliminary selection was made. Results of this study depict the available power sources in light of mission requirements as they are currently defined.

1991-09-02

211

Using Regulatory and Epistatic Networks to Extend the Findings of a Genome Scan: Identifying the Gene Drivers of Pigmentation in Merino Sheep

The first candidate rotavirus vaccine was a live attenuated oral vaccine made by the classical empirical method of serial passage of virus in tissue culture cells. Current tetravalent vaccine candidates that are in the final stages of efficacy testing in the United States were made by genetic reassortment. This article briefly highlights how advances in the basic understanding of the molecular biology of rotaviruses have facilitated vaccine development. New approaches for second-generation vaccines and improvements in vaccine efficacy based on further exploitation of the tools and knowledge of rotavirus molecular biology and pathogenesis are discussed. PMID:8752289

1996-09-01

212

Transposons for cancer gene discovery: Sleeping Beauty and beyond

Extending genome wide association analysis by the inclusion of gene expression data may assist in the dissection of complex traits. We examined piebald, a pigmentation phenotype in both human and Merino...Full Text Available

213

Transcriptome Profiling of a Toxic Dinoflagellate Reveals a Gene-Rich Protist and a Potential Impact on Gene Expression Due to Bacterial Presence

The use of Sleeping Beauty transposons as somatic mutagens to discover cancer genes in hematopoietic tumors and sarcomas has been documented. Here, we discuss the future of Sleeping...Full Text Available

2007-01-01

214

Transcription induces strand-specific mutations at the 5? end of human genes

BackgroundDinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear...Full Text Available

215

Trans-Species Polymorphism and Selection in the MHC Class II DRA Genes of Domestic Sheep

A regional analysis of nucleotide substitution rates along human genes and their flanking regions allows us to quantify the effect of mutational mechanisms associated with transcription in germ line...Full Text Available

2008-08-01

216

Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum.

Highly polymorphic genes with central roles in lymphocyte mediated immune surveillance are grouped together in the major histocompatibility complex (MHC) in higher vertebrates. Generally, across vertebrate...Full Text Available

217

The role of gene expression in ecological speciation

Rhizobium leguminosarum, biovar viceae, strain RCC1001 contains two glutamine synthetase activities, GSI and GSII. We report here the identification of glnA, the structural gene for GSI. A 2 kb fragment...Full Text Available

1987-03-11

218

The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei.

Ecological speciation is the process by which barriers to gene flow between populations evolve due to adaptive divergence via natural selection. A relatively unexplored area in ecological speciation...Full Text Available

2010-09-01

219

The red-green visual pigment gene region in adrenoleukodystrophy.

We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter...Full Text Available

1994-10-11

220

The opiorphin gene (ProL1) and its homologues function in erectile physiology

Although recent data established that a specific very-long-chain fatty acyl-CoA synthetase is defective in X-linked adrenoleukodystrophy (ALD), the ALD gene is still unidentified. The ALD locus has...Full Text Available

1990-03-01

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221

The high Andes, gene flow and a stable hybrid zone shape the genetic structure of a wide-ranging South American parrot

OBJECTIVETo determine if ProL1, a member of the opiorphin family of genes, can modulate erectile physiology, as it encodes a peptide which acts as...Full Text Available

2008-09-01

222

The complexity of gene expression dynamics revealed by permutation entropy

BackgroundWhile the gene flow in some organisms is strongly affected by physical barriers and geographical distance, other highly mobile species are able to overcome such constraints....Full Text Available

223

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.

BackgroundHigh complexity is considered a hallmark of living systems. Here we investigate the complexity of temporal gene expression patterns using the concept of Permutation Entropy...Full Text Available

224

The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

1983-10-25

225

The Characterisation of Three Types of Genes that Overlie Copy Number Variable Regions

Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen...Full Text Available

1994-12-01

226

Targeted gene transfection from microbubbles into vascular smooth muscle cells using focused, ultrasound-mediated delivery

BackgroundDue to the increased accuracy of Copy Number Variable region (CNV) break point mapping, it is now possible to say with a reasonable degree of confidence whether a gene...Full Text Available

227

Synthesis and degradation of dinoflagellate plastid-encoded psbA proteins are light-regulated, not circadian-regulated

We investigate a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles...Full Text Available

2010-09-01

228

Species-specific microRNA roles elucidated following astrocyte activation

In many dinoflagellate species, the plastid genome has been proposed to exist as a limited number of single-gene minicircles, and many genes normally found in the plastid genome are nuclear-encoded....Full Text Available

2005-02-22

229

Sequence variants of the DFNB31 gene among Usher syndrome patients of diverse origin

MicroRNAs (miRNAs) are short non-coding RNAs that play a central role in regulation of gene expression by binding to target genes. Many miRNAs were associated with the function of the central nervous...Full Text Available

2011-05-01

230

SLC25A4 - solute carrier family 25 (mitochondrial carrier; adenine...

PurposeIt has been demonstrated that mutations in deafness, autosomal recessive 31 (DFNB31), the gene encoding whirlin, is responsible for nonsyndromic hearing loss...Full Text Available

231

Role of Calcitonin Gene-Related Peptide in Bone Repair after Cyclic Fatigue Loading

The official name of this gene is "solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4." SLC25A4 is the gene's official symbol. The...

2011-08-13

232

Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae.

BackgroundCalcitonin gene related peptide (CGRP) is a neuropeptide that is abundant in the sensory neurons which innervate bone. The effects of CGRP on isolated bone cells have been...Full Text Available

233

Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex

The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile...Full Text Available

1993-08-01

234

Recent Applications of DNA Microarray Technology to Toxicology and Ecotoxicology

Brown fat is a specialized tissue that can dissipate energy and counteract obesity through a pattern of gene expression that greatly increases mitochondrial content and uncoupled respiration. PRDM16...Full Text Available

2008-05-15

235

Rai1 duplication causes physical and behavioral phenotypes in a mouse model of dup(17)(p11.2p11.2)

Gene expression is a unique way of characterizing how cells and organisms adapt to changes in the external environment. The measurements of gene expression levels upon exposure to a chemical can be...Full Text Available

2006-01-01

236

PrognoScan: a new database for meta-analysis of the prognostic value of genes

Genomic disorders are conditions that result from DNA rearrangements, such as deletions or duplications. The identification of the dosage-sensitive gene(s) within the rearranged genomic interval is...Full Text Available

2006-11-01

237

Practical Applications of the Bioinformatics Toolbox for Narrowing Quantitative Trait Loci

BackgroundIn cancer research, the association between a gene and clinical outcome suggests the underlying etiology of the disease and consequently can motivate further studies. The...Full Text Available

238

Power of grammatical evolution neural networks to detect gene-gene interactions in the presence of error

Dissecting the genes involved in complex traits can be confounded by multiple factors, including extensive epistatic interactions among genes, the involvement of epigenetic regulators, and the variable...Full Text Available

2008-12-01

239

Polymorphisms in the epidermal growth factor receptor gene and the risk of primary lung cancer: a case-control study

BackgroundWith the advent of increasingly efficient means to obtain genetic information, a great insurgence of data has resulted, leading to the need for methods for analyzing this...Full Text Available

240

Polymorphic Regions Affecting Human Height Also Control Stature in Cattle

BackgroundPolymorphisms in Epidermal Growth Factor Receptor (EGFR) gene may influence EGFR production and/or activity, thereby modulating susceptibility to lung...Full Text Available

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241

PEG-conjugated PAMAM Dendrimers Mediate Efficient Intramuscular Gene Expression

Orthologous positions of 55 genes associated with height in four human populations were located on the bovine genome. Single nucleotide polymorphisms close to eight of these genes were significantly...Full Text Available

2011-03-01

242

Overexpression of bacterial ethylene-forming enzyme gene in Trichoderma reesei enhanced the production of ethylene

Generations 5 and 6 (G5 and G6) poly(amidoamine) (PAMAM) dendrimers have been shown to be highly efficient nonviral carriers in in vitro gene delivery. However, their high toxicity...Full Text Available

243

Origins, evolution, and phenotypic impact of new genes

In order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from Pseudomonas...Full Text Available

244

On the spontaneous stochastic dynamics of a single gene: complexity of the molecular interplay at the promoter

Ever since the pre-molecular era, the birth of new genes with novel functions has been considered to be a major contributor to adaptive evolutionary innovation. Here, I review the origin and evolution...Full Text Available

2010-10-01

245

Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression

BackgroundGene promoters can be in various epigenetic states and undergo interactions with many molecules in a highly transient, probabilistic and combinatorial way, resulting in...Full Text Available

246

Neoplastic transformation of prostatic and urogenital epithelium by the polyoma virus middle T gene.

Noncoding RNAs play important roles in various aspects of gene regulation. We have identified 7SK RNA to be enriched in nuclear speckles or interchromatin granule clusters (IGCs), a subnuclear domain...Full Text Available

2010-12-01

247

Mutation analysis of the ferritin L-chain gene in age-related cataract

Male transgenic mice expressing the polyomavirus middle T (PyV-MT) gene exhibited growth and developmental abnormalities in prostatic and other urogenital epithelium. Expression of PyV-MT was directed...Full Text Available

1996-10-01

248

Multivariate analyses reveal common and drug specific genetic influences on responses to four drugs of abuse

PurposeTo investigate whether acquired somatic mutations in the iron response element of the ferritin L-chain gene account for the age-related cataract.MethodsThe...Full Text Available

249

Multiple Genes on Chromosome 7 Regulate Dopaminergic Amacrine Cell Number in the Mouse Retina

Vulnerability to abused drugs is influenced by multiple genes unique to each drug as well as to risk genes for polydrug abuse. If several inbred mouse strains respond to different drugs similarly,...Full Text Available

2008-11-01

250

Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32. 1

PurposeThe size of neuronal populations is modulated by gene variants that influence cell production and survival, in turn influencing neuronal connectivity, function,...Full Text Available

2009-05-01

251

Molecular cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

1994-05-15

252

Linking Gene Expression and Functional Network Data in Human Heart Failure

This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...Full Text Available

1989-11-01

253

Leptin receptor gene expression and number in the brain are regulated by leptin level and nutritional status

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

254

Kinetic Complexity of the Global Response to Glucocorticoid Receptor Action

Hormone potency depends on receptor availability, regulated via gene expression and receptor trafficking. To ascertain how central leptin receptors are regulated, the effects of leptin challenge, high-fat...Full Text Available

2009-07-15

255

Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene.

We have characterized the kinetic response of gene targets throughout the murine genome to transcriptional modulation by the glucocorticoid receptor (GR). In contrast to a model in which multiple genes...Full Text Available

2009-04-01

256

Influence of major histocompatibility complex genotype on mating success in a free-ranging reptile population

Exploring the possibility of enhancing the properties of baculoviruses as biological control agents of insect pests, we tested the effect of expressing an insect gene (jhe) encoding juvenile hormone...Full Text Available

1992-05-01

257

Inferring gene regulatory networks from asynchronous microarray data with AIRnet

Major histocompatibility complex (MHC) genes are highly polymorphic components of the vertebrate immune system, which play a key role in pathogen resistance. MHC genes may also function as odour-related...Full Text Available

2009-05-07

258

Incorporation of dUTP does not mediate mutation of A:T base pairs in Ig genes in vivo

BackgroundModern approaches to treating genetic disorders, cancers and even epidemics rely on a detailed understanding of the underlying gene signaling network. Previous work has...Full Text Available

259

Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis

Activation-induced cytidine deaminase (AID) protein initiates Ig gene mutation by deaminating cytosines, converting them into uracils. Excision of AID-induced uracils by uracil-N-glycosylase...Full Text Available

2010-12-01

260

Identification of the Haemophilus influenzae tolC Gene by Susceptibility Profiles of Insertionally Inactivated Efflux Pump Mutants

IntroductionPrevious observations suggest that active systemic juvenile idiopathic arthritis (sJIA) is associated with a prominent erythropoiesis gene-expression signature. The aim...Full Text Available

2010-01-01

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261

Identification of cell cycle-related regulatory motifs using a kernel canonical correlation analysis

Isogenic strains containing insertional disruptions of 10 Haemophilus influenzae Rd genes were investigated for their effects on the susceptibility of the organism to various classes...Full Text Available

2004-04-01

262

Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli.

BackgroundGene regulation is a key mechanism in higher eukaryotic cellular processes. One of the major challenges in gene regulation studies is to identify regulators affecting the...Full Text Available

263

Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription

A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase....Full Text Available

1990-04-01

264

Heterogeneous mutations in the human lipoprotein lipase gene in patients with familial lipoprotein lipase deficiency.

Cellular hypertrophy is regulated by coordinated pro- and antigrowth machineries. Foxo transcription factors initiate an atrophy-related gene program to counter hypertrophic growth. This study was designed...Full Text Available

2008-09-01

265

Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy

The DNA sequences were determined for the lipoprotein lipase (LPL) gene from five unrelated Japanese patients with familial LPL deficiency. The results demonstrated that all five patients are homozygotes...Full Text Available

1991-12-01

266

Genomic cloning and characterization of a ricin gene from Ricinus communis.

The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve ...

1994-03-01

267

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

268

Gene Signature For Predicting Survival Outcome Of Human Hepatocellular Carcinoma (HCC) - Technology Transfer Center

BackgroundDue to the high morbidity and mortality of fulminant hepatitis, early diagnosis followed by early effective treatment is the key for prognosis improvement. So far, little...Full Text Available

269

Fusion expression of pedA gene to obtain biologically active pediocin PA-1 in Escherichia coli *

The National Cancer Institute Laboratory of Human Carcinogenesis is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize a gene signature for prognosis of hepatocellular carcinoma in patients.

270

Functional Enhancers at the Gene-Poor 8q24 Cancer-Linked Locus

Two heterologous expression systems using thioredoxin (trxA) as a gene fusion part in Escherichia coli were developed to produce recombinant pediocin PA-1. Pediocin...Full Text Available

2011-01-01

271

Full genome gene expression analysis of the heat stress response in Drosophila melanogaster

Multiple discrete regions at 8q24 were recently shown to contain alleles that predispose to many cancers including prostate, breast, and colon. These regions are far from any annotated gene and their...Full Text Available

2009-08-01

272

Expression of embryonic hemoglobin genes in. cap alpha. -thalassemic and in. beta. -duplication mice

The availability of full genome sequences has allowed the construction of microarrays, with which screening of the full genome for changes in gene expression is possible. This method can provide a wealth...Full Text Available

2005-10-01

273

Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii?

The results of a study of the expression of embryonic hemoglobin genes in mice which show an imbalance of alpha and non-alpha chain synthesis are reported. (ACR)

1979-01-01

274

Drosophila melanogaster Methoprene-tolerant (Met) gene homologs from three mosquito species: members of PAS transcriptional factor family

Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR....Full Text Available

2009-06-01

275

Dose Optimization for Long-term rAAV-mediated RNA Interference in the Nigrostriatal Projection Neurons

The Methoprene-tolerant (Met) gene in Drosophila melanogaster has been shown to function in juvenile hormone (JH) action. Met...Full Text Available

2007-03-01

276

Dexamethasone and nitric oxide synthase gene expression in brain.

Short-hairpin RNA (shRNA)–mediated gene knockdown is a powerful tool for targeted gene silencing and an emerging novel therapeutic strategy. Recent publications, however, reported unexpected...Full Text Available

2009-09-01

277

Development of a novel data mining tool to find cis-elements in rice gene promoter regions

Systemic administration of lipopolysaccharide (LPS), which causes endotoxemia and systemic inflammation, has been reported to induce expression of the gene for type II inducible nitric oxide synthase...Full Text Available

1997-03-01

278

Coordination of PAD4 and HDAC2 in the regulation of p53 target gene expression

BackgroundInformation on more than 35 000 full-length Oryza sativa cDNAs, together with associated microarray gene expression data collected under various treatment...Full Text Available

279

Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma

Histone Arg methylation and Lys acetylation have been found to cooperatively regulate the expression of p53 target genes. Peptidylarginine deiminase 4 (PAD4) is an enzyme that citrullinates...Full Text Available

2010-05-27

280

Comparative genomics of the bacterial genus Listeria: Genome evolution is characterized by limited gene acquisition and limited gene loss

BackgroundPolyethyleneimine (PEI), which can interact with negatively charged DNA through electrostatic interaction to form nanocomplexes, has been widely attempted to use as a gene...Full Text Available

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281

Combinatorial Gene Regulation Using Auto-Regulation

BackgroundThe bacterial genus Listeria contains pathogenic and non-pathogenic species, including the pathogens L. monocytogenes and L. ivanovii,...Full Text Available

282

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

As many as 59% of the transcription factors in Escherichia coli regulate the transcription rate of their own genes. This suggests that auto-regulation has one or more important...Full Text Available

2010-06-01

283

Chronic Cocaine-Induced H3 Acetylation and Transcriptional Activation of CaMKII? in the Nucleus Accumbens Is Critical for Motivation for Drug Reinforcement

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

284

Chiropteran types I and II interferon genes inferred from genome sequencing traces by a statistical gene-family assembler

The regulation of gene expression in the brain reward regions is known to contribute to the pathogenesis and persistence of drug addiction. Increasing evidence suggests that the regulation of gene transcription...Full Text Available

2010-03-01

285

Characterization, phylogeny, alternative splicing and expression of Sox30 gene

BackgroundThe rate of emergence of human pathogens is steadily increasing; most of these novel agents originate in wildlife. Bats, remarkably, are the natural reservoirs of many...Full Text Available

286

Characterization of the H(+)-pumping F1F0 ATPase of Vibrio alginolyticus.

BackgroundMembers of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including...Full Text Available

287

Characterization of Mouse UDP-glucose Pyrosphosphatase, a Nudix Hydrolase encoded by the Nudt14 Gene

The F1F0 ATPase of Vibrio alginolyticus was cloned from a chromosomal lambda library. The unc operon, which contains the structural genes for the ATPase, was sequenced and shown to have a gene organization...Full Text Available

1990-12-01

288

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

Recombinant mouse UDP-glucose pyrophosphatase (UGPPase), encoded by the Nudt14 gene, was produced in Escherichia coli and purified close to homogeneity. The...Full Text Available

2009-12-25

289

Brain transcriptomic analysis in paper wasps identifies genes associated with behaviour across social insect lineages

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

290

Antisense imaging of gene expression in the brain in vivo

Comparative sociogenomics has the potential to provide important insights into how social behaviour evolved. We examined brain gene expression profiles of the primitively eusocial wasp Polistes...Full Text Available

2010-07-22

291

Analysis of the Type IV Fimbrial-Subunit Gene fimA of Xanthomonas hyacinthi: Application in PCR-Mediated Detection of Yellow Disease in Hyacinths

Antisense radiopharmaceuticals could be used to image gene expression in the brain in vivo, should these polar molecules be made transportable through the blood–brain barrier....Full Text Available

2000-12-19

292

An integrative multi-dimensional genetic and epigenetic strategy to identify aberrant genes and pathways in cancer

A sensitive and specific detection method was developed for Xanthomonas hyacinthi; this method was based on amplification of a subsequence of the type IV fimbrial-subunit gene fimA...Full Text Available

2001-02-01

293

A supervised approach for predicting patient survival with gene expression data

BackgroundGenomics has substantially changed our approach to cancer research. Gene expression profiling, for example, has been utilized to delineate subtypes of cancer, and facilitated...Full Text Available

294

A retinoid responsive cytokine gene, MK, is preferentially expressed in the proximal tubules of the kidney and human tumor cell lines.

Rapid development in genomics in recent years has allowed the simultaneous measurement of the expression levels of thousands of genes using DNA microarrays. This has offered tremendous potential...Full Text Available

2010-01-01

295

A polyacetylene compound from herbal medicine regulates genes associated with thrombosis in endothelial cells

The aim of this study was to survey the expression of an embryonic cytokine gene, MK, in the normal organs and neoplastic tissues of adults. Northern analysis showed that MK mRNA was exclusively expressed...Full Text Available

1993-02-01

296

A classification-based framework for predicting and analyzing gene regulatory response

From Toki-shakuyaku-san, an herbal formulation for “cleansing stagnated blood,” a key gene regulatory compound was purified and identified through a screening based on DNA microarray...Full Text Available

2007-12-15

297

A Predictive Risk Probability Approach for Microarray Data with Survival as an Endpoint

BackgroundWe have recently introduced a predictive framework for studying gene transcriptional regulation in simpler organisms using a novel supervised learning algorithm called...Full Text Available

298

5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.

Gene expression profiling has played an important role in cancer risk classification and has shown promising results. Since gene expression profiling often involves determination of a set of...Full Text Available

2008-01-01

299

SNP diversity of Enterococcus faecalis and Enterococcus faecium in a South East Queensland waterway, Australia, and associated antibiotic resistance gene profiles.

Synthetic complementary oligonucleotides are useful hybridization probes for the detection of mRNAs and genes encoding proteins for which only a partial amino acid sequence is known. Usually this involves...Full Text Available

1988-03-01

300

Regulation of the expression of the apolipoprotein(a) gene : Evidence for a regulatory role of the 5' distal ACR enhancer in YAC transgenic mice

PMID:21910889

2011-09-12

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301

Development of QTL Mapping Populations

No abstract prepared.

2003-01-30

302

Spamology: A Study of Spam Origins

The objective of this animation is to develop a QTL mapping population for locating and characterizing the genes responsible for resistance to tan spot disease of wheat.

303

Short rotation coppice with Robinia pseudoacacia L. : a land use option for carbon sequestration on reclaimed mine sites

The rise of spam in the last decade has been staggering, with the rate of spam exceeding that of legitimate email. While conjectures exist on how spammers gain access to email addresses to spam, most work in the area of spam containment has either focused on better spam filtering methodologies or on understanding the botnets commonly used to send spam. In this paper, we aim to understand the origins of spam. We post dedicated email addresses to record how and where spammers go to obtain email addresses. We find that posting an email address on public Web pages yields immediate and high-volume spam. Surprisingly, even simple email obfuscation approaches are still sufficient today to prevent spammers from harvesting emails. We also find that attempts to find open relays continue to be popular among spammers. The insights we gain on the use of Web crawlers used to harvest email addresses and the commonalities of techniques used by spammers open ...

2009-07-01

304

Radiation processing as a post-harvest quarantine control for raisins, dried figs and dried apricots

A study in northeast Germany has shown that the establishment of short rotation coppices (SRC) of Robinia pseudoacacia L. may be a viable option for improving farmers income on marginal soils. The plantations produce woody biomass at a fast rate for energy use. Carbon is accumulated in the harvestable biomass, as well as in the stump and the roots. These plant compartments form a long-term carbon storage pool because they can survive a harvest, stay vital at the site and continue to grow as the plant ages. As organic litter decomposes, additional carbon is sequestered under SRC as soil organic carbon. The carbon sequestration in SRC of R. pseudoacacia on mining sites within the Lower Lusatian region in northeast Germany was studied and the results were complemented with findings of current field studies conducted on reclaimed mine sites. The average above ground dry matter productivity of R. pseudoacacia was found to be 3 to 10 Mg per hectare ...

2010-07-01

305

Precooling techniques and applications for horticultural products - a review

The commercially packed samples of raisins, dried figs and dried apricots were irradiated using doses in the range of 0.5-1.0 kGy for disinfestation and 0.5-5.0 kGy for sensory analysis with the dose rate ranging from 1.44 to 1.92 kGy/h. Pests on dried fruits were evaluated after 0, 1, 2 and 3 months of storage for irradiated dried figs and 1, 3, 6 and 12 months of storage for raisins and dried apricots. Sensory analysis of dried figs, dried apricots and raisins were carried out after 0, 1, 3, 6 and 12 months of storage. The results indicated that radiation processing at low doses (#approx#1.0 kGy) is an effective post-harvest treatment and quarantine control for these products with no adverse effects on sensory (marketing) attributes.

2006-03-01

306

Investigations of biomimetic light energy harvesting pigments

One of the most important factors affecting the postharvest life and quality of horticultural crops is temperature. Quality loss after harvest occurs as a result of physiological and biological processes, the rates of which are influenced primarily by product temperature. As the maintenance of market quality is of vital importance to the success of the horticultural industry, it is necessary not only to cool the product but to cool it as quickly as possible after harvest. The process of precooling is the removal of field heat which arrest the deteriorative and senescence processes so as to maintain a high level of quality that ensures customer satisfaction. This paper provides a critical review to portray precooling as an intricate part of temperature management and to highlight the importance of its utilisation for extending the shelf life and maintaining the quality of horticultural products with emphasis on cut flowers. Various different ...

2001-07-01

307

An anthropological view of the forest culture of Peten, Guatemala

This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). Nature uses chlorophyll and other porphyrinic pigments to capture and transfer light energy as a preliminary step in photosynthesis. The design of synthetic assemblies of light harvesting and energy directing pigments has been explored through synthesis and characterization of porphyrin oligomers. In this project, pigment electronic and vibrational structures have been explored by electrochemistry and dynamic and static optical measurements. Transient absorption data reveal energy transfer between pigments with lifetimes on the order of 20--200 picoseconds, while Raman data reveal that the basic porphyrin core structure is unperturbed relative to the individual monomer units. These two findings, along with an extensive series of experiments on the oxidized oligomers, reveal that coupling between the pigments is ...

1998-12-01

308

beta-Lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands

Traditional farmers and harvesters of non-timber forest products in Peten treat the forest in ways that conserve and regenerate its resources. They believe no one owns the forest outright. Humans share it with other life forms and therefore no one has the right to monopolize or destroy forest resources. Traditional Peteneros find a use for almost everything in the forest, and believe it neither smart nor proper to use a given area for a single purpose, for example, to clear an area of all trees and devote it exclusively to a cattle pasture. In the traditional system most medicinal plants, basic foodstuffs, fuelwoods and construction materials are taken from the bosque (secondary forests within walking distance of human settlements). Peteneros` sustainable use of bosques eases pressure on the monte (primary forests). In the monte Peteneros` harvesting practices are protective of resources, causing minimal damage. Finding almost everything in the ...

1995-12-31

309

The k43 gene, required for chorion gene amplification and diploid cell chromosome replication, encodes the Drosophila homolog of yeast origin recognition complex subunit 2

Objectives: The purpose of this work was to study the genetic determinants responsible for extended-spectrum beta-lactamase (ESBL) resistance of Salmonella isolated from Dutch poultry, poultry meat and hospitalized humans. Methods: Thirty-four ESBL-resistant Salmonella isolates from The Netherlands were tested towards 21 antimicrobial agents. PCR and sequencing were used to determine the underlying genetic determinants responsible for the ESBL phenotypes. The transferability of the ESBL phenotypes was tested by conjugation to a susceptible Salmonella enterica serovar Dublin and plasmid purification, restriction fragment length polymorphism (RFLP) and pulsed-field gel electrophoresis (PFGE) were employed to further characterize a subset of the isolates. Results: A great genetic diversity was seen among the isolates. The bla(TEM-52) gene was most predominant and was found among Salmonella enterica serovars Blockley, Thomson, London, Enteritidis phage type 14b, ...

2005-01-01

310

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

Lethal alleles of the Drosophila k43 gene result in small or missing imaginal discs, greatly reduced mitotic index, and fragmented and abnormally condensed chromosomes. A female-sterile...Full Text Available

1997-04-15

311

SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in...Full Text Available

2004-07-01

312

Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

313

Organization of lin Genes and IS6100 among Different Strains of Hexachlorocyclohexane-Degrading Sphingomonas paucimobilis: Evidence for Horizontal Gene Transfer

We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1...Full Text Available

1993-01-01

314

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli

The organization of lin genes and IS6100 was studied in three strains of Sphingomonas paucimobilis (B90A, Sp+, and UT26) which degraded hexachlorocyclohexane...Full Text Available

2004-04-01

315

BackgroundThe large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity...Full Text Available

316

DNA repair genes

Conservation of B class gene expression in the second whorl of a basal grass and outgroups links the origin of lodicules and petals

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

317

Complementation of areA- regulatory gene mutations of Aspergillus nidulans by the heterologous regulatory gene nit-2 of Neurospora crassa.

Studies of flower development in core eudicot species have established a central role for B class MADS-box genes in specifying petal and stamen identities. Similarly in maize and rice, B class genes...Full Text Available

2007-01-16

318

Combined mutations of ASXL1, CBL, FLT3, IDH1, IDH2, JAK2, KRAS, NPM1, NRAS, RUNX1, TET2 and WT1 genes in myelodysplastic syndromes and acute myeloid leukemias

Loss-of-function mutations in the regulatory gene areA of Aspergillus nidulans prevent the utilization of a wide variety of nitrogen sources. The phenotypes of nit-2 mutants of Neurospora crassa suggest...Full Text Available

1987-06-01

319

Biodegradable Tri-Block Copolymer Poly(lactic acid)-poly(ethylene glycol)-poly(l-lysine)(PLA-PEG-PLL) as a Non-Viral Vector to Enhance Gene Transfection

BackgroundGene mutation is an important mechanism of myeloid leukemogenesis. However, the number and combination of gene mutated in myeloid malignancies is still a matter of investigation.MethodsWe...Full Text Available

320

A unique horizontal gene transfer event has provided the octocoral mitochondrial genome with an active mismatch repair gene that has potential for an unusual self-contained function

Low cytotoxicity and high gene transfection efficiency are critical issues in designing current non-viral gene delivery vectors. The purpose of the present work was to synthesize the novel biodegradable...Full Text Available

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321

`Ecological forestry' and eucalypt forests managed for wood production in south-western Australia

BackgroundThe mitochondrial genome of the Octocorallia has several characteristics atypical for metazoans, including a novel gene suggested to function in DNA repair. This mtMutS...Full Text Available

322

British Library Electronic Table of Contents (United Kingdom)

The model of `ecological forestry' has evolved as a part of the development of the concept of ecosystem management. `Ecological forestry' emphasises that manipulation of a forest ecosystem should consider, and as far as practicable work within the limits of, natural disturbance patterns prior to extensive human alteration of the landscape. This paper evaluates the extent to which forest management practices in jarrah (Eucalyptus marginata) and karri (Eucalyptus diversicolor) forests of south-western Australia align with this view of the characteristics and appropriate silviculture of `ecological forestry'. Characteristics and appropriate silviculture of `ecological forestry' are evaluated in relation to (i) the stand level decisions of stand structure and harvest timing and (ii) the landsc...

2007-01-01

323

Wastage of stump wood in terms of energy in spruce (Picea smithiana) and silver fir (Abies pindrow) forests

Methods to improve the fertility of poinsettia

A study of two forest areas in India investigated how much energy is wasted when high stumps are left after trees are cut to produce wooden boxes for packing the apple harvest. Measurements of the stumps determined the total wood pulp wasted, which was 1191 tons during 1981. The calorific value of the stumps would supply a year's cooking and heating fuels for 747 families of five. Additional fuel is wasted by abandoning the branches and tree tops which are unsuitable for box construction. 5 references, 4 tables.

1984-11-01

324

British Library Electronic Table of Contents (United Kingdom)

`Success' or `Red Splendor' plants propagated by shoot tip culture or embryogenesis had higher pollen germination than those propagated by cutting. All clones of `Pepride', `Peter Star' and `Nobel Star' propagated by cutting or by embryogenesis or shoot tip culture did not have viable pollen. `Peter Star' mutants through mutagenesis could recover pollen viability. When both parents were propagated by tissue culture, fruit set increased compared to when one or both parents were propagated by cuttings. Furthermore, the number of the harvested seeds doubled per pollination set, when the tissue culture parents were used instead of cutting-propagation plants.

2008-01-01

325

Fluidized-bed energy technology for biomass conversion

A perspective of the Malaysian highway energy consumption and future power supply

Fluidized bed technology was experimentally evaluated for the combustion and gasification of cotton gin waste. The isothermal bed temperatures in the reactors could be maintained below the ash fusion point of the waste. Raw stripper harvested cotton gin trash could be metered directly into the fluidized-bed reactors indicating that little feed preparation is required. H and CO were the primary products of gasification, and approximately 3200-400 Btu of gas were produced per lb of cotton gin waste. These techniques offer the potential of providing small-scale energy conversion systems for use on farms.

1980-01-01

326

British Library Electronic Table of Contents (United Kingdom)

In this short communication, we discuss the energy consumption trends in the Malaysian road transport sector, with a special emphasis on the energy losses due to vehicle aerodynamic drag on highways. The recent trends of energy consumption in the Malaysian road transport sector are reviewed. It is evidently shown that the aerodynamic losses represented exceed 1.2MTOE annually since 2002. A novel concept of vertical-axis wind turbine (VAWT) farms for harvesting aerodynamic energy losses on Malaysian highways is preliminarily proposed. The novel concept aims at providing a sustainable and green energy source for the lighting of the highway network in the country.

2011-01-01

327

Switchgrass ultimate stresses at typical biomass conditions available for processing

Supramolecular Structures for Photochemical Energy Conversion

Biomass tensile and shear ultimate failure stresses were measured with the aim of identifying biomass 'weakest mode of failure' or 'natural fracture point' as a basis for future grinder designs. Switchgrass (Panicum virgatum L.) ultimate stresses were determined for Alamo and Kanlow varieties over ranges in maturity and moisture content. Alamo had greater ultimate tensile stress than Kanlow (P=0.0091), with mean values of 97.8 and 89.7MPa, respectively. Alamo had greater ultimate shear stress than Kanlow (P=0.0091), with mean values of 20.5 and 17.9MPa, respectively. Shear was the 'weakest mode of failure'. Grinders that use knives, shear bars, and mechanical pinch points that apply opposed-sliding actions are expected to be more energy efficient. Mean ultimate tensile stress and shear stress were significantly different between switchgrass varieties. A survey of failure stresses for a range of biomass feedstocks is recommended for ...

2006-03-15

328

Quality of wooden chips produced by Claas Jaguar fitted with experimental CRA-ING rotor

OAK B188 The goal of this project is to mimic the energy transduction processes by which photosynthetic organisms harvest sunlight and convert it to forms of energy that are more easily used and stored. The results may lead to new technologies for solar energy harvesting based on the natural photosynthetic process. They may also enrich our understanding and control of photosynthesis in living organisms, and lead to methods for increasing natural biomass production, carbon dioxide removal, and oxygen generation. In our work to date, we have learned how to make synthetic antenna and reaction center molecules that absorb light and undergo photoinduced electron transfer to generate long-lived, energetic charge-separated states. We have assembled a prototype system in which artificial reaction centers are inserted into liposomes (artificial cell-like constructs), where they carry out light-driven transmembrane translocation of hydrogen ions to ...

2003-08-26

329

Analysis of the direct contamination pathway of "8"5Sr, "1"0"3Ru and "1"3"4Cs in radish

This paper described a newly developed chipper device for mounting on Claas Jaguar chipper harvesters for short rotation forestry harvesting. It was designed by the Agriculture Engineering Research Unit of the Agricultural Research Council in Rome, Italy. The new rotor has a lower number of blades compared to standard rotors and has a different cutting angle and drum insertion. This study evaluated the particle-size distribution of wood chips produced by standard rotors compared to those with the experimental rotor. The objective was to distinguish the particle size distribution of chips produced using the two types of chippers in order to evaluate the influence of wooden species in the chip formation process. Tests were conducted using 860 and 890 Claas Jaguar chipper harvesters, both equipped with standard and CRA-ING rotors. The experiment was conducted on poplar cultivations on biennial shift and on arboreal ...

2010-07-01

330

Selective downregulation of retinoic acid-inducible gene I within the intestinal epithelial compartment in crohn's disease

For analyzing the direct contamination pathway of the radionuclide in radish, a solution containing "8"5Sr, "1"0"3Ru and "1"3"4Cs was sprayed to the aerial part of the plant in a greenhouse at 5 different times before harvest. Plant interception factor showed little difference among radionuclides and increased with decreasing time interval between application and harvest with the maximum value of 0.86. The fractions of the initial deposition that remained in the radish plant at harvest were in the range of 16#approx#37% for "8"5Sr, 15#approx#68% for "1"0"3Ru and 35#approx#58% for "1"3"4Cs. The remaining fraction was the highest in "1"3"4Cs at earlier applications while it was the highest in "1"0"3Ru at later applications. Translocation factors of "8"5Sr, "1"0"3Ru and "1"3"4Cs were in the range of 3.2x10"-"3#approx#1.7x10"-"2 , 7.3x10"-"4#approx#2.6x10"-"2 and 6.6x10"-"2#approx#1.7x10"-"2, respectively, in upper root and in ...

2000-05-01

331

British Library Electronic Table of Contents (United Kingdom)

AbstractBackground: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD). Methods: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25). Results: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) with...

2011-01-01

332

Low cytotoxicity effect of dendrosome as an efficient carrier for rotavirus VP2 gene transferring into a human lung cell line

British Library Electronic Table of Contents (United Kingdom)

The efficiency of dendrosome (a gene porter) was assessed in transferring recombinant human rotavirus VP2 cDNA into A549, a human lung cell line. After gene transferring, transmission electron microscopy showed core-like particles (CLPs) formation in the transfected cells both with dendrosome and lipofectamine porters. In addition, western blotting analysis showed that the expression of VP2 gene was almost equal in the dendrosome and lipofectamine-transfected cells. Also, the cytotoxicity studies revealed that dendrosome had a lower cytotoxicity than lipofectamine. Therefore, our study may introduce dendrosome as a possible carrier for gene transferring into the human lung cell line, especially, for intranasally administration of DNA vaccines.

2009-01-01

333

Dendrosomes as novel gene porters-III

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND: It was previously reported that dendrosomes, i.e. neutral, biodegradable, covalent or self-assembled, hyperbranched, spheroidal nano-particles with a size ranging from 15 to 100 nm, provide a convenient and efficient means of gene delivery into various kinds of cells such as human hepatoma and kidney cells as well as animal models.RESULTS: New studies via circular dichroism show that hydrophilic and amphipathic dendrosomes either do not affect the DNA structure or moderately transform it from B- to A-conformation. Gene delivery into human liver, kidney, and endothelial cells as well as other animal cells like Bowes, U-937, Raw, CCRF-CEM, MOLT-4, K562, Huh-7 and VERO reveal that the genes are efficiently expressed and in comparison with other gene porters like Lipofectin or bact...

2008-01-01

334

Chromosomal rearrangement segregating with adrenoleukodystrophy: A molecular analysis

Chromosomal localization and structure of the human type II IMP dehydrogenase gene

The relationship between X chromosome-linked adrenoleukodystrophy and the red/green color pigment gene cluster on Xq28 was investigated in a large kindred. The DNA in a hemizygous male showed altered restriction fragment sizes compatible with at least a deletion extending from the 5[prime] end of the color pigment genes. Segregation analysis using a DNA probe within the color pigment gene cluster showed significant linkage with adrenoleukodystrophy (logarithm of odds score of 3.19 at [theta] = 0.0). These data demonstrate linkage, rather than association, between a unique molecular rearrangement in the color pigment gene cluster and adrenoleukodystrophy. The DNA changes in this region are thus likely to be helpful for determining the location and identity of the responsible gene. 33 refs., 4 figs.

1993-10-15

335

Cardiac Channelopathies and Sudden Infant Death Syndrome

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.

1994-05-01

336

British Library Electronic Table of Contents (United Kingdom)

Abstract Sudden infant death syndrome (SIDS) is always a devastating and unexpected occurrence. SIDS is the leading cause of death in the first 6 months after birth in the industrialized world. Since the discovery in 1998 of long QT syndrome as an underlying substrate for SIDS, around 10-20% of SIDS cases have been proposed as being caused by genetic variants in either ion channel or ion channel-associated proteins. Until now, 10 cardiac channelopathy susceptibility genes have been found to be implicated in the pathogenesis of SIDS. Four of the genes encode cardiac ion channel a-subunits, 3 genes encode ion channel b-subunits, and 3 genes encode other channel-interacting proteins. All 10 genes have been associated with primary electrical heart diseases. SIDS may hereby be the initial sympt...

2011-01-01

337

Sequences homologous to the human x- and y-borne zinc finger protein genes (ZFX/Y) are autosomal in monotreme mannals

Molecular studies of the uncoupling protein

The human zinc finger protein genes (ZFX/Y) were identified as a result of a systematic search for the testis-determining factor gene on the human Y chromosome. Although they play no direct role in sex determination, they are of particular interest because they are highly conserved among mammals, birds, and amphibians and because, in eutherian mammals at least, they have active alleles on both the X and the Y chromosomes outside the pseudoautosomal region. We used in situ hybridization to localize the homologues of the zinc finger protein gene to chromosome 1 of the Australian echidna and to an equivalent position on chromosomes 1 and 2 of the playtpus. The localization to platypus chromosome 1 was confirmed by Southern analysis of a Chinese hamster [times] platypus cell hybrid retaining most of platypus chromosome 1. This localization is consistent with the cytological homology of chromosome 1 between the two species. The ...

1993-02-01

338

A systematic search for novae in M31 on a large set of digitized archival Schmidt plates

The uncoupling protein (UCP) is a proton/anion transporter found in the inner mitochondrial membrane of brown adipocyte. Although UCP has nor been detected in mitochondria from any other tissue, it shares structural and catalytic properties with several other mitochondrial carrier proteins. Although UCP was discovered only recently it is one of the most extensively studied mitochondrial carrier proteins.More recently, the mouse, rat, and human genes encoding for UCP have been isolated and sequenced. The availability of these various tools has led to several significant observations. UCP gene expression is strongly controlled at the level of transcription by signals that are activated after the stimulation of brown adipocytes by norepinephrine. The comparison of UCP gene with the genes encoding the adenine nucleotide translocator revealed the existence of structural and evolutionary homologies. Moreover, ...

1991-06-01

339

A comprehensive Network Security Risk Model for process control networks.

This paper reports on the detection of optical novae in our neighbour galaxy M31 based on digitized historical Tautenburg Schmidt plates. The accurate positions of the detected novae lead to a much larger database for searches for recurrent novae in M31. We conducted a systematic search for novae on 306 digitized Tautenburg Schmidt plates covering a time span of 36 years from 1960 to 1996. From the database of both ~ 300 000 light curves and about one million detections on only one plate per colour band, nova candidates were efficiently selected by automated algorithms and subsequently individually inspected by eye. We report the detection of 84 nova candidates. In detail we found 55 nova candidates from the automated analysis of the light curves. Among these, 22 were previously unknown, 12 were known but have not been identified on Tautenburg Schmidt plates before and 21 novae have been discovered on Tautenburg plates ...

2007-01-01

340

Characterization of the human lipoprotein lipase (LPL) promoter: Evidence of two cis-regulatory regions, LP-[alpha] and LP-[beta] of importance for the differentation-linked induction of the LPL gene during adipogenesis

The risk of cyber attacks on process control networks (PCN) is receiving significant attention due to the potentially catastrophic extent to which PCN failures can damage the infrastructures and commodity flows that they support. Risk management addresses the coupled problems of (1) reducing the likelihood that cyber attacks would succeed in disrupting PCN operation and (2) reducing the severity of consequences in the event of PCN failure or manipulation. The Network Security Risk Model (NSRM) developed in this article provides a means of evaluating the efficacy of candidate risk management policies by modeling the baseline risk and assessing expectations of risk after the implementation of candidate measures. Where existing risk models fall short of providing adequate insight into the efficacy of candidate risk management policies due to shortcomings in their structure or formulation, the NSRM provides model structure and ...

2008-10-24

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341

Screening study of mixed transition-metal oxides for use as cathodes in thermal batteries

When preadipocytes differentiate into adipocytes, several differentiation-linked genes are activated. Lipo-protein lipase (LPL) is one of the first genes induced during this process. To investigate early events in adipocyte development, we have focused on the transcriptional activation of the LPL gene. For this purpose, we have cloned and fused different parts of intragenic and flanking sequences with a chloramphenicol acetyltransferase reporter gene. Transient transfection experiments and DNase I hypersensitivity assays indicate that several positive as well as negative elements contribute to transcriptional regulation of the LPL gene. When reporter gene constructs were stably introduced into preadipocytes, we were able to monitor and compare the activation patterns of different promoter deletion mutants at selected time points representing the process of ...

1992-10-01

342

Oxidation/sulfidation of material candidates for distributed solar receiver thermochemical transport program in SO/sub 2//O/sub 2/

Over 100 candidates were examined, including commercial materials and many that were synthesized in house. The mixed oxides were based on Ti, V, Nb, Cr, Mo, W, Mn, Fe, Co, Ni, and Cu doped with other transition metals. A number of individual (single-metal) oxides were included for comparison. The candidates were tested in single cells with Li(Si) anodes and separators based on LiCl-KCl eutectic. Screening was done under constant-current conditions at current densities of 125 me/cm{sup 2} and, to a lesser extent, 50 me/cm{sup 2} at 500 C. Relative performance and limitations of the oxide cathodes are discussed.

1996-05-01

343

Analysis of the creep strain-time behavior of alloy 800

Metals for potential use in the dissociator and the synthesizer reactors of a distributed solar receiver thermochemical transport loop utilizing SO/sub 3//SO/sub 2//O/sub 2/ molecular chemistry were tested in SO/sub 2//O/sub 2/ mixtures at 500/sup 0/C and 900/sup 0/C, respectively, for times of up to four weeks. They included titanium, aluminum and nickel, and iron-base, nickel-base and cobalt-base superalloys. Weight gain measurements determine the oxidation/sulfidation kinetics. Electron microprobe analysis identifies any possible penetration of oxygen and sulfur into the metal and the formation of internal oxides and sulfides. The most promising candidates as a result of these tests are the cobalt-base superalloys.

1985-06-01

344

The mouse formin (Fmn) gene: Genomic structure, novel exons, and genetic mapping

The high-nickel austenitic alloy 800 (in both the mill-annealed and the solution-treated grades) has several attractive properties that make it a good candidate for service attractive properties that make it a good candidate for service at elevated temperatures in corrosive environments. One such property is creep resistance. This report analyzes the elevated-temperature creep behavior of the mill-annealed grade, generally referred to simply as alloy 800. (The solution-treated grade is known as alloy 800H). Available data over the temperature range from 538 to 760/sup 0/C were collected and evaluated to yield mathematically approximations for creep-rupture and strain-time behavior for use in design calculations. However, the creep behavior of this material is extremely complex, and the analysis presented here contains substantial uncertainties. All results in this report should be considered preliminary because of limited data currently ...

1983-05-01

345

Mapping cis-Regulatory Domains in the Human Genome UsingMulti-Species Conservation of Synteny

Mutations in the mouse formin (Fmn) gene, formerly known as the limb deformity (ld) gene, give rise to recessively inherited limb deformities and renal malformations or aplasia. The Fmn gene encodes many differentially processed transcripts that are expressed in both adult and embryonic tissues. To study the genomic organization of the Fmn locus, we have used Fmn probes to isolate and characterize genomic clones spanning 500 kb. Our analysis of these clones shows that the Fmn gene is composed of at least 24 exons and spans 400 kb. We have identified two novel exons that are expressed in the developing embryonic limb bud as well as adult tissues such as brain and kidney. We have also used a microsatellite polymorphism from within the Fmn gene to map it genetically to a 2.2-cM interval between D2Mit58 and D2Mit103. 36 refs., 6 figs., 1 tab.

1997-02-01

346

Identification of three related human GRO genes encoding cytokine functions

Our inability to associate distant regulatory elements with the genes that they regulate has largely precluded their examination for sequence alterations contributing to human disease. One major obstacle is the large genomic space surrounding targeted genes in which such elements could potentially reside. In order to delineate gene regulatory boundaries we used whole-genome human-mouse-chicken (HMC) and human-mouse-frog (HMF) multiple alignments to compile conserved blocks of synteny (CBS), under the hypothesis that these blocks have been kept intact throughout evolution at least in part by the requirement of regulatory elements to stay linked to the genes that they regulate. A total of 2,116 and 1,942 CBS>200 kb were assembled for HMC and HMF respectively, encompassing 1.53 and 0.86 Gb of human sequence. To support the existence of complex long-range regulatory domains within these CBS we ...

2005-06-13

347

Cluster Analysis of Gene Expression Data

The product of the human GRO gene is a cytokine with inflammatory and growth-regulatory properties; GRO is also called MGSA for melanoma growth-stimulatory activity. The authors have identified two additional genes, GRO#beta# and GRO#gamma#, that share 90% and 86% identity at the deduced amino acid level with the original GRO#alpha# isolate. One amino acid substitution of proline in GRO#alpha# by leucine in GRO#beta# and GRO#gamma# leads to a large predicted change in protein conformation. Significant differences also exist in the 3' untranslated region, including different numbers of ATTTA repeats associated with mRNA instability. A 122-base-pair region in the 3' region is conserved among the three GRO genes, and a part of it is also conserved in the Chinese hamster genome, suggesting a role in regulation. DNA hybridization with oligonucleotide probes and partial sequence analysis of the genomic clones confirm that the ...

348

Cloning and mapping of the mouse {alpha}7-neuronal nicotinic acetylcholine receptor

The expression levels of many thousands of genes can be measured simultaneously by DNA microarrays (chips). This novel experimental tool has revolutionized research in molecular biology and generated considerable excitement. A typical experiment uses a few tens of such chips, each dedicated to a single sample - such as tissue extracted from a particular tumor. The results of such an experiment contain several hundred thousand numbers, that come in the form of a table, of several thousand rows (one for each gene) and 50 - 100 columns (one for each sample). We developed a clustering methodology to mine such data. In this review I provide a very basic introduction to the subject, aimed at a physics audience with no prior knowledge of either gene expression or clustering methods. I explain what genes are, what is gene expression and how it is measured by DNA chips. Next I explain what ...

2002-01-01

349

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

We report the isolation of cDNA clones for the mouse {alpha}7 neuronal nicotinic acetylcholine receptor subunit (gene symbol Acra7), the only nicotinic receptor subunit known to bind a-bungarotoxin in mammalian brain. This gene may have relevance to nicotine sensitivity and to some electrophysiologic findings in schizophrenia. The mouse {alpha}7 subunit gene encodes a protein of 502 amino acids with substantial identity to the rat (99.6%), human (92.8%), and chicken (87.5%) amino acid sequences. The {alpha}7 gene was mapped to mouse chromosome 7 near the p locus with the following gene order from proximal to distal: Myod1-3.5 {+-}1.7 cM-Gas2-0.9 cM {+-} 0.9 cM-D7Mit70-1.8 {+-} 1.2 cM- Acra7-4.4 {+-}1.0 cM-Hras1-ps11/Igf1r/Snrp2a. The human gene was confirmed to map to the homologous region of human chromosome 15q13-q14. 26 refs., 3 figs.

1995-03-20

350

[Cloning of the gene for thermostable Thermus aquaticus YT1 DNA polymerase and its expression in Escherichia coli].

We have isolated a genomic clone containing 'Lupinus luteus' 5S ribosomal RNA genes by screening with 5S rDNA probe clones that were hybridized previously with the initiator methionine tRNA preparation (contaminated) with traces of rRNA or its degradation products). The clone isolated contains ten repeat units of 342 bp with 119 bp fragment showing 100% homology to the 5S rRNA from yellow lupine. Sequence analysis indicates only point heterogeneities among the flanking regions of the genes. (author). 6 refs, 3 figs.

1993-01-01

351

Unmasking Stem/Progenitor Cell Properties in Differentiated ...

Using the phasmid vector pSL5, the genomic DNA fragment of T. aquaticus YT1 which contained the thermostable DNA polymerase (Taq-polymerase) gene was cloned. The BglII fragment of this genome locus was subcloned in the BamHI site of the pUC19 plasmid. To optimize the Taq-polymerase gene expression in E. coli cells, the gene was cloned in the correct reading frame regarding the initiation ATG codon of the pPR-TGATG-1 expression vector. The gene expression in this vector was controlled by the phage lambda PR promoter and the temperature-sensitive phage lambda repressor. We used PCR to amplify the short 5'-end fragment of the Taq-polymerase gene coding for the part into which an artificial SacI site was introduced. This site has been used for cloning the PCR product into the pPR-TGATG-1 vector, and the missing gene part was cloned into the KpnI site of the PCR ...

352

The innate immune repertoire in Cnidaria - ancestral complexity and stochastic gene loss

... In addition to changes in BrdU, we also observed transient changes in p63 gene expression in the myoepithelial/stem cell layer. ...

2007-08-01

353

The burden of genetically determined eye disease.

BackgroundCharacterization of the innate immune repertoire of extant cnidarians is of both fundamental and applied interest - it not only provides insights into the basic immunological...Full Text Available

2007-01-01

354

The DFNA5 gene, responsible for hearing loss and involved in cancer, encodes a novel apoptosis-inducing protein

We determined the underlying aetiology of blindness for the registered blind population of the Province of Newfoundland and Labrador. In both 1981 and 1984 single-gene disorders accounted for 30% of...Full Text Available

1986-09-01

355

British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

2011-01-01

356

The Arc of synaptic memory

Sleeping Beauty Transposition From Nonintegrating Lentivirus

The immediate early gene Arc is emerging as a versatile, finely tuned system capable of coupling changes in neuronal activity patterns to synaptic plasticity, thereby optimizing information...Full Text Available

2010-01-01

357

Sequence features involved in the mechanism of 3' splice junction wobbling

Lentiviral vectors enter cells with high efficiency and deliver stable transduction through integration into host chromosomes, but their preference for integration within actively transcribing genes...Full Text Available

2009-07-01

358

Science@Berkeley Lab Magazine

BackgroundAlternative splicing is an important mechanism mediating the diversified functions of genes in multicellular organisms, and such event occurs in around 40-60% of human...Full Text Available

359

Recent Advances in Lentiviral Vector Development and Applications

will soon recreate by slamming lead nuclei into one another. S@BL image Irrelevant Regulators Pinpointing the interactions of genes with their assumed regulators grows ever more...

2011-08-19

360

Lentiviral vectors (LVs) have emerged as potent and versatile vectors for ex vivo or in vivo gene transfer into dividing and nondividing cells. Robust phenotypic correction...Full Text Available

2010-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Ras history

Probiotic-Induced Priming of Innate Immunity to Protect Against Rotaviral Infection

Although the roots of Ras sprouted from the rich history of retrovirus research, it was the discovery of mutationally activated RAS genes in human cancer in 1982 that stimulated an...Full Text Available

2010-07-01

362

British Library Electronic Table of Contents (United Kingdom)

Understanding of probiotic-induced regulatory gene expression and networking is critical to further explore their roles in controlling infection. Transcriptional profile of selected innate immune genes in primary bovine intestinal epithelial cells was assessed over a time course of incubation with the probiotic Lactobacillus plantarum 299v. Based on gene expression results, a time point was chosen to prime epithelial cells with the probiotic prior to infection with rotavirus. Plaque assays and genomic analysis provided the basis for establishing the efficacy of probiotics in preventing a rotaviral infection. Plaque assays revealed that the probiotic is capable of decreasing (at least by 100-fold) the levels of live virus when the cells were primed with the probiotic. Results from gene expr...

2010-01-01

363

Prestin and high frequency hearing in mammals

Photorhabdus luminescens genes induced upon insect infection

Recent evidence indicates that the evolution of ultrasonic hearing in echolocating bats and cetaceans has involved adaptive amino acid replacements in the cochlear gene prestin. A substantial...Full Text Available

2011-03-01

364

Ovine reference materials and assays for prion genetic testing

BackgroundPhotorhabdus luminescens is a Gram-negative luminescent enterobacterium and a symbiote to soil nematodes belonging to the species Heterorhabditis...Full Text Available

365

Ovarian Gene Expression is Stable after Exposure to Trichloroethylene

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

366

Norepinephrine represses the expression of toxA and the siderophore genes in Pseudomonas aeruginosa

Exposure of female rats to trichloroethylene (TCE), an environmental toxicant commonly found in ground and surface waters throughout the United States, reduces the fertilizability of oocytes...Full Text Available

2008-02-28

367

Molecular Genetics and Carcinogenesis Section

Among the different extracellular virulence factors produced by Pseudomonas aeruginosa are exotoxin A (ETA) and the pyoverdine and pyochelin siderophores. Production of ETA...Full Text Available

2009-10-01

368

Microarray-based gene expression profiles of silkworm brains

The Molecular Genetics and Carcinogenesis Section conducts studies using human epithelial cells to assess: activation of proto-oncogenes by chemical and physical carcinogens; inactivation and dysregulation of tumor suppressor genes by chemical and physical

369

Mapping of the human cone transducin {alpha}-subunit (GNAT2) gene to 1p13 and negative mutation analysis in patients with Stargardt disease

BackgroundMolecular genetic studies of Bombyx mori have led to profound advances in our understanding of the regulation of development. Bombyx mori brain,...Full Text Available

370

Male Reproductive Proteins and Reproductive Outcomes

We report localization of the human cone transducin (GNAT2) gene using fluorescence in situ hybridization on chromosome 1 in band p13. The recent assignment of a gene for Stargardt disease to the same chromosomal region by linkage analysis prompted us to investigate the possible role of GNAT2 in the pathogenesis of this disease. We investigated 66 unrelated patients for mutations in the coding region of the GNAT2 gene using polymerase chain reaction-single strand conformation polymorphism analysis (SSCP) and direct sequencing. No disease-specific mutations were found, indicating that GNAT2 is probably not involved in the pathogenesis of most cases of Stargardt disease. 19 refs., 1 fig., 1 tab.

1995-01-01

371

KMeyeDB: a graphical database of mutations in genes that cause eye diseases

Male reproductive proteins (MRPs), associated with sperm and semen, are the moieties responsible for carrying male genes into the next generation. Evolutionary biologists have focused on their...Full Text Available

2008-06-01

372

British Library Electronic Table of Contents (United Kingdom)

KMeyeDB () is a database of human gene mutations that cause eye diseases. We have substantially enriched the amount of data in the database, which now contains information about the mutations of 167 human genes causing eye-related diseases including retinitis pigmentosa, cone-rod dystrophy, night blindness, Oguchi disease, Stargardt disease, macular degeneration, Leber congenital amaurosis, corneal dystrophy, cataract, glaucoma, retinoblastoma, Bardet-Biedl syndrome, and Usher syndrome. KMeyeDB is operated using the database software MutationView, which deals with various characters of mutations, gene structure, protein functional domains, and polymerase chain reaction (PCR) primers, as well as clinical data for each case. Users can access the database using an ordinary Internet browser wi...

2010-01-01

373

Human and rat mast cell high-affinity immunoglobulin E receptors: Characterization of putative. alpha. -chain gene products

The authors have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative {alpha} subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH{sub 2}-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hydrophobic transmembrane region, and a positively charged cytoplasmic tail. The human and rat {alpha} subunits share an overall homology with one another and the immunoglobulin gene family, suggesting that they arose from a common ancestral gene and continue to share structural homology with their ligands. In addition, the rat gene is transcribed into at least three distinct forms, each of which yields a somewhat different coding sequence.

1988-03-01

374

GrainGenes 2.0

Genomic imprinting and the social brain

Proceedings of the 11th IWGS Proceedings of the 8th IOC TREP, Release 10 Barley QTL Community Curation Workbook CIMMYT International Nursery Data Brachypodium website Rye...

2011-10-01

375

Gene repression by minimal lac loops in vivo

Genomic imprinting refers to the parent-of-origin-specific epigenetic marking of a number of genes. This epigenetic mark leads to a bias in expression between maternally and paternally inherited imprinted...Full Text Available

2006-12-29

376

Gene Networks and the Neuroendocrine Regulation of Puberty

The inflexibility of double-stranded DNA with respect to bending and twisting is well established in vitro. Understanding apparent DNA physical properties in vivo is...Full Text Available

2010-12-01

377

Expression of an ampG like gene in Herbaspirillum seropedicae strain SmR1 is regulated by naringenin and its product appears to be involved in the determination of LPS structure

A sustained increase in pulsatile release of gonadotrophin releasing hormone (GnRH) from the hypothalamus is an essential, final event that defines the initiation of mammalian puberty. This...Full Text Available

2010-08-05

378

Evaluating Phylogenetic Congruence in the Post-Genomic Era

No abstract prepared.

2009-07-01

379

Effect of an Ostertagia ostertagi infection on the transcriptional stability of housekeeping genes in the bovine abomasum

Congruence is a broadly applied notion in evolutionary biology used to justify multigene phylogeny or phylogenomics, as well as in studies of coevolution, lateral gene transfer, and as evidence for...Full Text Available

2011-01-01

380

British Library Electronic Table of Contents (United Kingdom)

Quantitative Real-Time PCR (qRT-PCR) is a widely used tool to study host responses against parasites. A crucial step in the gene quantification process is the normalization of the expression data against stable housekeeping genes (HKGs). However, in recent years, several reports have showed that the transcriptional levels of such HKGs can change dramatically, especially when cellular changes appear in the tissues investigated. The aim of the current study was to assess the variability of 11 putative HKGs in bovine abomasal tissue during an infection with the parasitic nematode Ostertagia ostertagi. Gene transcription levels of selected potential HKGs were measured by qRT-PCR and the expression stabilities evaluated using geNorm, NormFinder, and The Mann-Whitney-U test. The analysis showed ...

2011-01-01

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381

Development of a Suite of Luciferase Gene Probes for the ...

Detection of polychlorinated biphenyl degradation genes in polluted sediments by direct DNA extraction and polymerase chain reaction

... between 2 strains is acquired by adding the lengths of the connecting branches, using the scale which depicts 1 amino acid substitution per 10 ...

2011-05-15

382

Defective gut function in drop-dead mutant Drosophila

It was the aim of this study to specifically detect the DNA sequences for the bphC gene, the meta-cleavage enzyme of the aerobic catabolic pathway for biphenyl and polychlorinated biphenyl degradation, in aquatic sediments without prior cultivation of microorganisms by using extraction of total DNA, PCR amplification of bphC sequences, and detection with specific gene probes. The direct DNA extraction protocol used was modified to enhance lysis efficiency. Crude extracts of DNA were further purified by gel filtration, which yielded DNA that could be used for the PCR. PCR primers were designed for conserved regions of the bphC gene from a sequence alignment of five known sequences. The specificity of PCR amplification was verified by using digoxigenin-labeled DNA probes which were located internal to the amplified gene sequence. The detection limit for the bphC gene of Pseudomonas ...

1993-12-01

383

DNA Analysis of mtDNA COI Gene in the Sharp-toothed Eel (Muraenesox cinereus Forskal) from Yeosu, Jinhae, Jeju, Goseoung, Jangheung and Haenam Populations in Korea Using PCR-aided RFLP

Mutation of the gene drop-dead (drd) causes adult Drosophila to die within 2 weeks of eclosion and is associated with reduced rates of defecation...Full Text Available

2009-09-01

384

Conjugative Botulinum Neurotoxin-Encoding Plasmids in Clostridium botulinum

Full Text Available

385

Conditional Circadian Regulation of PHYTOCHROME A Gene Expression

BackgroundClostridium botulinum produces seven distinct serotypes of botulinum neurotoxins (BoNTs). The genes encoding different subtype neurotoxins of serotypes...Full Text Available

386

Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha (Insecta: Hemiptera: Heteroptera)

The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides...Full Text Available

2001-12-01

387

Cholinesterase for Prophylactics of Poisoning by Organophosphorous Inhibitors

BackgroundNucleotide sequences and the gene arrangements of mitochondrial genomes are effective tools for resolving phylogenetic problems. Hemipteroid insects are known to possess...Full Text Available

388

International Science & Technology Center (ISTC)

The Production of Highly Active Human Blood Plasma Butyrylcholinesterase Preparation by Gene Engineering Methods to Create Protective Means against Poisoning by Organophosphorous Cholinesterase Inhibitors

389

Characterization of cry Genes in a Mexican Bacillus thuringiensis Strain Collection

Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution

Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis...Full Text Available

1998-12-01

390

Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats

The DNA sequence motif ATTTGCAT (octamer) or its inverse complement has been identified as an evolutionarily conserved element in the promoter region of immunoglobulin genes. Two major DNA-binding proteins that bind in a sequence-specific manner to the octamer DNA sequence have been identified in mammalian species--a ubiquitously expressed protein (Oct-1) and a lymphoid-specific protein (Oct-2). During characterization of the promoter region of the chicken immunoglobulin light chain gene, the authors identified two homologous octamer-binding proteins in chicken B cells. when the cloning of the human gene for Oct-2 revealed it to be a member of a distinct family of homeobox genes, they sought to determine if the human Oct-2 cDNA could be used to identify homologous chicken homeobox genes. Using a human Oct-2 homeobox-specific DNA probe, they were able to identify 6-10 ...

1990-02-01

391

British Library Electronic Table of Contents (United Kingdom)

To test the hypothesis that transduction of the channelrhodopsin-2 (ChR2) gene, a microbial-type rhodopsin gene, into retinal ganglion cells of genetically blind rats will restore functional vision, we recorded visually evoked potentials and tested the experimental rats for the presence of optomotor responses. The N-terminal fragment of the ChR2 gene was fused to the fluorescent protein Venus and inserted into an adeno-associated virus to make AAV2-ChR2V. AAV2-ChR2V was injected intravitreally into the eyes of 6-month-old dystrophic RCS (rdy/rdy) rats. Visual function was evaluated six weeks after the injection by recording visually evoked potentials (VEPs) and testing optomotor responses. The expression of ChR2V in the retina was investigated histologically. We found that VEPs could not b...

2010-01-01

392

Cancer gene discovery in mouse and man

Browse Conditions - Genetics Home Reference

AbstractThe elucidation of the human and mouse genome sequence and developments in high-throughput genome analysis, and in computational tools, have made it possible to profile entire...Full Text Available

2009-12-01

393

Australian experience with herbicide tolerant (HT) and Bacillus thuringiensis (Bt) cotton

Home A service of the U.S. National Library of Medicine® Home Conditions Genes Chromosomes Handbook Glossary Resources Genetic Conditions > Browse Conditions 1-9 | A | B | C...

2011-09-24

394

British Library Electronic Table of Contents (United Kingdom)

Australia?s nationally consistent framework for gene technology regulation is underpinned by the Gene Technology Act 2000, administered by an independent decision-maker, the Gene Technology Regulator. The object of the Act is ?to protect the health and safety of people, and to protect the environment, by identifying risks posed by or as a result of gene technology, and by managing those risks through regulating certain dealings with genetically modified organisms?. Marketing and trade impacts are outside the scope of assessments required by the Act. Since 2001, seven licences have been issued for the commercial cultivation of genetically modified (GM) cotton with insect resistance and/or herbicide tolerance. Licences have also been issued for 32 GM cotton field trials with a broader range ...

2011-01-01

395

Association and expression study of synapsin III and schizophrenia

Artificial and natural duplicates in pyrosequencing reads of metagenomic data

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter...Full Text Available

2009-11-20

396

ALK, the Key Gene for Gelatinization Temperature, is a Modifier Gene for Gel Consistency in Rice

BackgroundArtificial duplicates from pyrosequencing reads may lead to incorrect interpretation of the abundance of species and genes in metagenomic studies. Duplicated reads were...Full Text Available

397

British Library Electronic Table of Contents (United Kingdom)

Abstract Gelatinization temperature (GT) is an important parameter in evaluating the cooking and eating quality of rice. Indeed, the phenotype, biochemistry and inheritance of GT have been widely studied in recent times. Previous map-based cloning revealed that GT was controlled by ALK gene, which encodes a putative soluble starch synthase II-3. Complementation vector and RNAi vector were constructed and transformed into Nipponbare mediated by Agrobacterium. Phenotypic and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for GT. Meanwhile, amylose content, gel consistency and pasting properties were also affected in transgenic lines. Two of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for GT. ...

2011-01-01

398

A systematic RNAi screen reveals involvement of endocytic pathway in neuronal dysfunction in a-synuclein transgenic C. elegans

British Library Electronic Table of Contents (United Kingdom)

Mutations or multiplications in a-synuclein gene cause familial forms of Parkinson disease or dementia with Lewy bodies (LB), and the deposition of wild-type a-synuclein as LB occurs as a hallmark lesion of these disorders, collectively referred to as synucleinopathies, implicating a-synuclein in the pathogenesis of synucleinopathy. To identify modifier genes of a-synuclein-induced neurotoxicity, we conducted an RNAi screen in transgenic C. elegans (Tg worms) that overexpress human a-synuclein in a pan-neuronal manner. To enhance the RNAi effect in neurons, we crossed a-synuclein Tg worms with an RNAi-enhanced mutant eri-1 strain. We tested RNAi of 1673 genes related to nervous system or synaptic functions, and identified 10 genes that, upon knockdown, caused severe growth/motor abnormalit...

2008-01-01

399

A child with hyperferritinemia: Case report

A Hybrid Multi Objective Particle Swarm Optimization Method to Discover Biclusters in Microarray Data

Hereditary hyperferritinemia cataract syndrome (HHCS) is a rare condition caused by mutations in the gene coding for the light chain of ferritin; it does not lead to iron overload, but it is associated...Full Text Available

400

Study of the mineral variation of two varieties of pigeonpea (Cajanus cajan (L.) Millsp) submitted to different doses of fertilizers by the neutron activation analysis; Acompanhamento da variacao mineral de duas cultivares de guandu (Cajanus cajan (L.) Millsp) submetidas a diferentes doses de fertilizantes, pelo metodo de analise por ativacao com neutrons

In recent years, with the development of microarray technique, discovery of useful knowledge from microarray data has become very important. Biclustering is a very useful data mining technique for discovering genes which have similar behavior. In microarray data, several objectives have to be optimized simultaneously and often these objectives are in conflict with each other. A Multi Objective model is capable of solving such problems. Our method proposes a Hybrid algorithm which is based on the Multi Objective Particle Swarm Optimization for discovering biclusters in gene expression data. In our method, we will consider a low level of overlapping amongst the biclusters and try to cover all elements of the gene expression matrix. Experimental results in the bench mark database show a significant improvement in both overlap among biclusters and coverage of elements in the gene expression matrix.

2009-01-01

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401

Effect of gamma radiation in the conservation of minimally processed cassava-parsley (Arracacia xanthorrhiza Bancroft) packed under vacuous

Instrumental neutron activation analysis (INAA) followed by gamma-ray spectrometry was used to determine the concentrations of As, Ca, Co, Cr, Cu, Eu, Fe, K, La, Mg, Mn, Mo, Na, Pr, Sb, Se, Th, U, V and Z, in 82 leaf samples belonging to two cultivars of pigeonpea (Cajanus cajan (L.) Millsp). Pigeonpea, an easy cultivation legume species, probably originated from Africa and adapted to brazilian conditions, is a very promising and unconventional source of food, mainly to the grain production intended for human feeding, and that even can be used in flour products. It is an excellent proteic supplement for ruminants, due to its large potential of forage production and its high nutritive value, or in addition it can be used in the recuperation of exhausting soils either by means of its utilization as green manure or by taking free Nitrogen from the atmosphere to produce nitrates which can be utilized by the plants. However, in spite of the several possibilities and increasing utilization ...

2001-07-01

402

Woody biomass production costs in the United States: An economic summary of commercial Populus plantation systems

In the third millennium, when the main words are globalization, world net of computers, genetic code and efficiency, deep transformations have been incorporated to human behavior. Among such transformations it may be pointed out, matching with the objectives of this study, the modem consumer's profile. The consumers have quickly become more and more conscious and demanding regarding to the quality of the available products in the market. In that sense, the consumer's concern about the natural and healthy appearance, as well as the preservation of the nutritious content of the foods, have been a constant sign of alert for producers and suppliers. Besides, the timeless for domestic preparation of foods have imposed the use of minimally processed as an incontestable reality. A barrier for the production of minimally processed foods is imposed by the degree of perishability of certain products, such as the cassava-parsley. This vegetable is a source of energy, calcium, phosphorus and ...

403

VIS harvesting unsymmetrical squaraine dye for dye-sensitized solar cells

Production costs for commercial-sized Populus plantations were developed from a series of research programs sponsored by the US Department of Energy's Short Rotation Woody Crops Program. Populus hybrid planted on good quality agricultural sites at a density of 2100 cuttings ha{sup {minus}1} was projected to yield an average of 16 Mg (OD) ha{sup {minus}1}yr{sup {minus}1}. Discounted cash flow analysis of multiple rotations showed pre-harvest production costs of $17 (US) Mg{sup {minus}1}(OD). 19 refs., 1 fig., 3 tabs.

1990-01-01

404

British Library Electronic Table of Contents (United Kingdom)

An unsymmetrical squaraine dye which contains an arylamino group was synthesized and used in dye-sensitized solar cells. The molar extinction coefficient of the dye is 77793 M-1 cm-1. Because our synthesized molecule may have no diradical character or the contribution of the diradicaloid component to resonance is quite small, the newly designed squaraine dye has a maximum absorption at 546 nm in the visible region compared with the NIR squaraine sensitizers. Cyclic voltammetry and time dependent density function theory calculation were accomplished to scrutinize the sensitized performance of the dye. Meanwhile, the novel sensitizer has been used to sensitize nanocrystalline TiO2-based solar cell. Under standard global AM 1.5 solar conditions, the squaraine dye-sensitized cell gives a short...

2012-01-01

405

The vascular pattern and viability of microvascularized rib grafts based on periosteal circulation--an experimental study

The endophytic fungi from wheat (Triticum aestivum L.)

Previous reports have stressed the importance of the nutrient blood supply in rib grafts transferred by microvascular anastomoses. In the present experimental study, we have demonstrated that a rib graft transferred by microvascular anastomoses based on periosteal vessels can survive; vascular clearing studies demonstrated that the vascularity of these grafts extends not only into the cortex but the medulla as well. The relative facility of harvesting these grafts (compared with those based on nutrient vessels) should make them the favored choice. Technetium bone scintigraphy proved accurate in the assessment of both vascular pattern and microanastomotic patency. Tetracycline labeling did not correlate well with the patency of a rib graft's pedicle blood supply.

1984-11-01

406

British Library Electronic Table of Contents (United Kingdom)

In order to study the species composition of endophytes from wheat healthy plants in Buenos Aires Province (Argentina) and to determine their infection frequencies from leaves, stems, glumes and grains, wheat plants were collected from five cultivars at five growth stages from crop emergence to harvest. A total of 1,750 plant segments (leaves, stems, glumes and grains) were processed from the five wheat cultivars at five growth stages, and 722 isolates of endophytic fungi recovered were identified as 30 fungal genera. Alternaria alternata, Cladosporium herbarum, Epicoccum nigrum, Cryptococcus sp., Rhodotorula rubra, Penicillium sp. and Fusarium graminearum were the fungi that showed the highest colonization frequency (CF%) in all the tissues and organs analysed. The number of taxa isolated...

2007-01-01

407

Selection of ancillary data to derive production management units in sweet corn (Zea Mays var. rugosa) using MANOVA and an information criterion

British Library Electronic Table of Contents (United Kingdom)

In production systems where high-resolution harvest data are unavailable there is often a reliance on ancillary information to generate potential management units. In these situations correct identification of relevant sources of data is important to minimize cost to the grower. For three fields in a sweet corn production system in central NSW, Australia, several sets of high-resolution data were obtained using soil and crop canopy sensors. Management units were derived by k-means classification for 2?5 classes using three approaches: (1) with soil data, (2) with crop data and (3) a combination of both soil and crop data. Crop quantity and quality were sampled manually, and the sample data were related to the different management units using multivariate analysis of variance (MANOVA). The ...

2011-01-01

408

Retinue of the beans roots growth by using neutron radiography technique

Report on repetition analyses for pesticide residues: 1988-1995; Rapporto sulle revisioni di analisi per residui di antiparassitari-1995

Agricultural practices frequently cause the development of a soil compacted layer below the surface. These compacted layers restrict the root penetration into deeper layers of soil, in search for water. It is proposed to monitor, using Non Destructive Test, the roots growth due to the planting of standard seeds in different agricultural soils, in function of their compactness and humidity. It will be used the neutrons beams derived from an irradiation channel called J-9 of the Reactor Argonauta (IEN/CNEN), so that the neutron radiographic images of the soil-plant system can be obtained. Each root can be evaluated for its ability to penetrate into compacted soil layers; this fact would mean an optimization of agricultural harvests. (author)

2002-08-11

409

Quantitative and qualitative aspects of agricultural products

From 1988 to 1995, 1,254 analyses were carried out on samples of fruits (61%), vegetables (29%), cereals and derived products (3%). The analyses were for 80 different pesticides, of which 51% were fungicides, 31% insecticides, 8% diphenylamine and ethoxiquin (post-harvest antioxidans agents for protection of fruits), and 5% antigermogliants and herbicides. Regions that mostly contributed with samples were: Emilia-Romagna (35%), Piedmont (15%), Liguria (11%), Tuscany (10%). Global rate of confirmation between first analysis and repetition analysis was 64% for all the samples analysed.

1995-12-01

410

Prediction of agrochemical residue data on fruit using an informatic system (PARDIS model)

The following aspects will be discussed: The main function of the Division of Inspection Services and its role in the Marketing of agricultural products; the shelf life of agricultural products: short review of present methods with a practical example of losses incurred due to limited keeping quality; irradiation and heat treatment: advantages of inhibited microbiological activities and undesirable chemical changes from a quality control point of view; quality standards: the basic principles of quality control; consequences of effective post-harvest treatment: export of deciduous and citrus fruit; the magnitude of the problem of poor keeping quality and quality requirements; other fruit; quantities and export limitations and vegetables; quantity and quality requirements; local marketing: fruit and vegetables subjected to inspection; quantity and quality aspects.

411

British Library Electronic Table of Contents (United Kingdom)

A `step-by-step' method was used to develop a simplified procedure for calculating pesticide residue levels on fruit at harvest by considering the application of the compound and the relevant routes of loss. The model is applicable to cases where the most important exposure route is by direct spray to the canopy of the crop and where uptake into the plant by the roots can be disregarded. The exposure dose is calculated by considering the proportion of total crop cover represented by the fruits. The loss processes considered are photodegradation, uptake, volatilization and washoff. The outputs of the model were compared with measured residues of pesticides on pear. Analysis of the model fit demonstrates that the model predicted the measured data with a good level of accuracy for four of sev...

2008-01-01

412

Local Perception of Environmental Change in a Semi-Arid Area of Northeast Brazil: A New Approach for the Use of Participatory Methods at the Level of Family Units

British Library Electronic Table of Contents (United Kingdom)

The diversity of plant resources in the Brazilian semi-arid region is being compromised by practices related to agriculture, pastures, and forest harvesting, especially in areas containing Caatinga vegetation (xeric shrublands and thorn forests). The impact of these practices constitutes a series of complex factors involving local issues, creating a need for further scientific studies on the social-environmental dynamics of natural resource use. Through participatory methods, the present study analyzed people?s representations about local environmental change processes in the Brazilian semi-arid region, taking into consideration local production systems, natural resources, and their importance. Environmental historical graphs were developed with nine local families to analyze landscape cha...

2011-01-01

413

Investigation of Tumor-Peritoneal Interactions in the Pathogenesis of Peritoneal Metastases using a Novel Ex Vivo Peritoneal Model1

British Library Electronic Table of Contents (United Kingdom)

BackgroundPeritoneal metastasis occurs in up to 30% of patients with gastric cancer. The aim of this experimental study is to develop and validate a novel ex vivo model of the human peritoneum to better identify factors involved in the development of peritoneal metastasis in order to improve its management and prognosis. MethodsPeritoneal discs harvested from hernia sacs obtained at inguinal hernia surgery were suspended in media using Teflon rings. Viability of the tissue was investigated using MTS assay, light and scanning electron microscopy (LM and SEM) over 72 h. To assess validity of the model, phenotypic changes in tumor cells were investigated. Changes in matrix metalloproteinases (MMP)-2 and -9 activities in HGC and AGS gastric adenocarcinoma cells after co-culture were investigat...

2010-01-01

414

International Union of Forest Research Organization 19th world congress proceedings

Infection by mycotoxigenic fungal species and mycotoxin contamination of maize grain in Umbria, central Italy

An international conference on forestry research presented papers on topics including forest mensuration, growth and yield; growth/yield relationships; design, performance, and evaluation of experiments; forest resource inventory and monitoring techniques; forest management, planning, and managerial economics; forestry and rural development in developing countries; forestry in sustainable economic development; conceptual advances in multiple-use forestry evaluations; economic evaluation of forest damages; supply and demand of roundwood and forest industry products; forestry and rural development in industrialized countries; analysis and evaluation of forest policies; forest institutions and organizations; biological and economic considerations when harvesting young thinning stands; applied sector modelling; and structural changes of the forest sector in a global perspective. Separate abstracts have been prepared for 3 papers from this conference.

1990-01-01

415

British Library Electronic Table of Contents (United Kingdom)

Surveys were carried out in 2006 and 2007 in Umbria (central Italy) to evaluate the presence of mycotoxigenic fungi and mycotoxins in maize grain sampled at harvest. Fusarium spp., were the most abundant species detected in maize kernels, followed by Aspergillus species of sections Flavi and Nigri and by Penicillium spp. Among Fusarium species, F. verticillioides was the most prevalent species, as detected by PCR directly on the kernels and on the fungi isolated from the kernels, followed by F. proliferatum and F. subglutinans. Fumonisins were the predominant mycotoxins with values, on average, of 4.3 and 5.7mgkg-1, in 2006 and 2007, respectively, with a maximum of 76.3mgkg-1 in the second year. Deoxynivalenol ranged from 0.2 to 3.98mgkg-1 in 2006 (average 1.04mgkg-1) and from undetectable...

2011-01-01

416

Importance of serum source for the in vitro replicative senescence of human bone marrow derived mesenchymal stem cells

British Library Electronic Table of Contents (United Kingdom)

Abstract Human mesenchymal stem cells (hMSCs) may be used for therapeutic applications. Culture conditions such as the serum source may impact on cell quality and the onset of replicative senescence. We have examined the effect of culturing hMSCs in autologous serum (AS) versus fetal bovine serum (FBS) on factors involved in in vitro replicative senescence. hMSCs from four donors were cultured in 10% FBS or 10% AS until they reached senescence. Cells were harvested at early passage and near senescence to study factors known to be involved in cellular senescence. The number of population doublings till senescence was similar for cells cultured in FBS, but varied greatly for hMSCs cultured in AS. FBS cells accumulated in S phase of cell cycle. This could not be explained by increased express...

2011-01-01

417

High hydrostatic pressure processing reduces Salmonella enterica serovars in diced and whole tomatoes

British Library Electronic Table of Contents (United Kingdom)

Fresh and fresh-cut tomatoes have been associated with numerous outbreaks of salmonellosis in recent years. One effective post harvest treatment to reduce Salmonella enterica in tomatoes may be high pressure processing (HPP). The objectives of the study were to determine the potential for HPP to reduce S. enterica serovars Newport, Javiana, Braenderup and Anatum in tryptic soy broth (TSB) and to determine the effect of HPP to reduce the most pressure resistant of the four serovars from fresh diced and whole tomatoes. To evaluate pressure resistance, TSB containing 8 log CFU/ml of one of the four serovars was packaged in sterile stomacher bags and subjected to one of three different pressures (350, 450 or 550MPa) for 120s. The most pressure resistant S. enterica serovar evaluated was Braend...

2011-01-01

418

From the harvest into the fermenter. Influence of the storage on the gas yield; Von der Ernte bis in den Fermenter. Einfluss der Lagerung auf die Gasausbeute

Energy recovery by production of fuel from citrus wastes

Main driver of the operation costs of agricultural biogas plants are substrate costs. The assessment of the biogas potential of different crops is carried out after ensiling and related to the DM content of the silage. This is insufficient for practical economic calculation due to prior dry mass losses during ensiling and aerobic deterioration. By the use of balancing trials process related dry mass losses can be estimated. In dependence of the deterioration time methane losses of 7,2 - 25,9% were evaluated. The methane losses match with the dry mass losses calculated according to Honig. (orig.)

2009-07-01

419

Effect of processing of dates into date juice concentrate and appraisal of its quality characteristics

A study to determine how much energy can be recovered from a Florida citrus processing plant was conducted. The production of ethyl alcohol in particular was examined as it is thought to represent the greatest potential for immediate energy recovery. Three-fourths of the energy expended to produce, harvest, process and market a box of fruit was recoverable using existing technology, i.e. 78,500 Btu/ box of fruit recoverable from a total energy expenditure of 107,800 Btu/ box of fruit. Aside from the actual cost benefits of recovering energy in the form of ethanol, the food processor is also helping to reduce the foreign-oil imports by the blending of ethyl alcohol with unleaded gasoline to form gasohol.

1982-05-01

420

British Library Electronic Table of Contents (United Kingdom)

Date palm (Phoenix dactylifera) is widely cultivated in Kutch district of Gujarat and the fruits are harvested at immature stage before the onset of monsoon to prevent spoilage. The immature date fruits with less commercial value were used for processing into date juice concentrate. Immature dates were crushed and treated with 0.1% pectinase enzyme for 120 min to obtain maximum juice. Date juice was found to be rich in reducing sugars (16.1%) and total sugars (18.3%). Juice was pasteurized at 85?C to inactivate the enzyme, cooled and centrifuged at 3000 rpm to get clear juice. The juice was concentrated in a thin film evaporator to a total soluble solids (TSS) of 76?Brix in 2 passes. Chemical composition of date juice during different stages of concentration was determined. Date juice conc...

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Determination of gamma radiation dose as a post-harvest treatment in mangos infested with the South American fruit fly

Curative resection of a huge malignant pancreatic endocrine tumor by pancreatoduodenectomy with portal and superior mesenteric vein resection and reconstruction using the right ovarian vein: Report of a case

Efforts are being made to determine a gamma radiation dose for mortality of third-instar larvae of Anastrepha fraterculus which infest mangos of the Haden variety of 400 g weight. Four radiation treatments were tested: 0.4 kGy, 0.6 kGy, 0.8 kGy and 1.0 kGy. Using as a criterium for mortality the interruption of the biological cycle between larva and pupa, the following results were achieved: 49.61%, 63.33%, 74.86% and 90.72%. The percentages obtained have been corrected using the Abbot formula. When the criterium was based on no adult emergence, 100% mortality was achieved for the four treatments. (author)

1999-01-01

422

British Library Electronic Table of Contents (United Kingdom)

Nonfunctioning pancreatic endocrine tumors (PETs) are rare and generally asymptomatic. A 68-year-old woman who had refused treatment for a pancreatic mass, revealed by ultrasonography to be 55 mm in diameter, was referred to us again 29 months later with jaundice. Investigations showed an 82-mm tumor in the head of pancreas, exposed from the papilla of Vater to the duodenal lumen. After biliary decompression and drainage, we performed pancreatoduodenectomy with resection of the portal vein and superior mesenteric vein, followed by reconstruction using a cylindrically customized autologous graft harvested from the right ovarian vein. The tumor was resected curatively. Microscopically, it consisted of trabecular and ribbon-like arrangement of neoplastic cells. Immunohistochemical staining wa...

2011-01-01

423

Chemical cotton stalk destruction for maintenance of host-free periods for the control of overwintering boll weevil in tropical and subtropical climates

British Library Electronic Table of Contents (United Kingdom)

In the Lower Rio Grande Valley (LRGV) of Texas, cotton regrows and produces fruit from undestroyed stalks throughout the winter, and in spring weevils from such locations become a serious threat. The success of the boll weevil eradication program, which was reintroduced in the LRGV in 2005, will be dependent on thorough stalk destruction following harvest. However, adverse weather conditions and conservation tillage often impede immediate and complete stalk destruction using typical tool implements, and alternative stalk control methods are needed. This study provides an examination of the efficacy for cotton stalk destruction of different herbicides (thifensulfuron-methyl + tribenuron-methyl, dicamba-diolamine, 2,4-D-dimethylammonium, flumioxazin, 2,4-DB-dimethylammonium and carfentrazone...

2007-01-01

424

Biological pest control

A review of the effect of different application rates on pesticide residue levels in supervised residue trials

For the purposes of energy forests, biological pest control should be interpreted as any method of using natural organisms or their products for the regulation of herbivores, below the economic threshold. The organisms include the energy forest crop species and natural enemies of pest herbivores. Examples are provided of three-trophic-level interactions, including some on willows and poplars. Bottom-up effects may be particularly strong in juvenile Salicaceae, which are used in energy forest production, because plants are growing vigorously, and many herbivores respond positively. Some major pests on willows and poplars appear to have weak top-down regulation, and they may be influenced most by bottom-up effects such as plant resistance through genetic variation, ontogenetic aging and physiological aging. Balancing the bottom-up and top-down forces in energy forests may be difficult in short-rotation crops, but harvesting may prove to be important as a control ...

1994-01-01

425

British Library Electronic Table of Contents (United Kingdom)

Abstract Residue trial data reported by the Joint FAO/WHO Meeting on Pesticide Residues (JMPR) have been reviewed to establish whether or not the resulting residues in harvested commodities are proportional to the pesticide application rate used on the crop. Numerous sets of trials were identified where the only parameter varied was application rate or spray concentration. Analysis of this database in terms of application rate, spray concentration, formulation type, preharvest interval, crop, pesticide, residue level and application type confirms that residues scale with application rate (proportionality principle). It is anticipated that use of the proportionality principle by regulators and those interested in evaluating pesticide residue data will improve pesticide risk assessment. Copy...

2011-01-01

426

The development and testing of emissivity enhancement coatings for thermophotovoltaic (TPV) radiator applications

Survey of degradation modes of candidate materials for high-level radioactive-waste disposal containers

One requirement of a thermophotovoltaic (TPV) radiator is to efficiently emit photons at high temperatures to TPV cells for conversion to electric power. Because many candidate radiator materials with adequate structural properties display low emissivity, coatings or other surface modifications are required for enhancement of emissivity. Six plasma sprayed coatings and one textured surface demonstrated adequate thermal stability and emittance values of 0.8 or greater. Promising attributes of modified surfaces are identified.

1999-03-01

427

Stress Corrosion Cracking Susceptibility of Beta Titanium Alloy 38-6-44: Candidate Alloy for Scout Torsion Bar.

Three copper-based alloys, CDA 102 (oxygen-free, high-purity copper), CDA 613 (aluminum bronze), and CDA 715 (Cu-30Ni), are candidates for the fabrication of high-level radioactive-waste disposal containers. Waste will include spent fuel assemblies from reactors as well as borosilicate glass, and will be sent to the prospective repository site at Yucca Mountain in Nye County, Nevada. The decay of radionuclides will result in the generation of substantial heat and in fluxes of gamma radiation outside the containers. In this environment, container materials might degrade by atmospheric oxidation, general aqueous phase corrosion, localized corrosion (LC), and stress corrosion cracking (SCC). This volume is a critical survey of available data on pitting and crevice corrosion of the copper-based candidates. Pitting and crevice corrosion are two of the most common forms of LC of these materials. Data on the SCC of these alloys is surveyed in Volume ...

1991-07-01

428

Spinning tachyons as particle constituents

The threshold stress itensities for stress corrosion crack propagation in beta titanium alloy 38-6-44, Ti-3Al-8V-6Cr-4Mo-4Zr, has been determined in salt water and methanolic solutions. The alloy was immune to stress corrosion cracking in aqueous sodium c...

1974-01-01

429

RMIT - Zahra, Dr. Louiseann

The motions of a classical free spinning point particle are extended to include the range of superluminal velocities. There is no formal change in the constraint-dependent functional relationship between observable mass and spin, i.e., trajectory, accompanying this extension. However, the theory now permits the specification, for superluminal velocities, of a linearly rising trajectory and naturally yields tachyon confinement. Therefore, unlike their subluminal counterparts, these tachyons can be considered candidates for elementary particle constituents.

430

Wastenet

... Zahra-Kingrsquo;s practice utilizes a range of media including textiles, metal casting, glass, sound, film and photography. Qualifications English Major from BA (Social Science) 1991; BA(Visual Arts) 1991; Grad. Dip. (Fine Art - Printmaking) 1993; Candidate for MFA 2001; Ph.D 2005. School of Art Research Clusters Art and Environmental Sustainability Art, ...

431

Pd-Na/F double exploding foil photoionization experiment

Optimal selection of capacitors for radial distribution systems using a genetic algorithm

The achievement of inner-shell photoionization of Zn-like Pd by means of two exploding foils is described. This resulted in the soft X-ray transition 3d94s2-3d104p at 46 A. It is shown that the photoionization of inner shell electrons in highly stripped ions can lead to X-ray transitions which may be candidates for X-ray lasers under 50 A.

1990-03-01

432

Ocean disposal feasibility study: candidate DOE (FUSRAP) soil characterization. [Formerly Utilized Sites Remedial Action Program

In this paper, a new design methodology for determining the size, location, type and number of capacitors to be placed on a radial distribution system is presented. The objective is to minimize the peak power losses and the energy losses in the distribution system considering the capacitor cost. A sensitivity analysis based method is used to select the candidate locations for the capacitors. A new optimization method using a Genetic Algorithm is proposed to determine the optimal selection of capacitors. Test results have been presented along with the discussion of the algorithm.

1994-08-01

433

New insights in LV torsion for the selection of cardiac resynchronisation therapy candidates

The Formerly Utilized Sites Remedial Action Program (FUSRAP) is to evaluate the radiological conditions at former MED-US AEC sites. Purpose of the Ocean FUSRAP program is to assess the feasibility of ocean disposal of FUSRAP waste which contains trace natural radioactive materials. This paper presents soil characterization information on the Middlesex, NJ, Sampling Plant site, and reports preliminary ocean dispersion calculations. (DLC)

1982-01-01

434

British Library Electronic Table of Contents (United Kingdom)

Recent literature indicates that torsion of the left ventricle (LV) is a promising predictor for response to cardiac resynchronisation therapy (CRT). Among patients with severe heart failure, 45 to 75% of patients show rigid body rotation, where the base and apex rotate in the same direction, instead of normal, opposite rotation. The occurrence of this phenomenon seems to be a good indicator for response to CRT. From this review, it can be concluded that LV torsion might be a welcome addition to current selection criteria.

2011-01-01

435

Metalloporphyrin catalysts for oxygen reduction developed using computer-aided molecular design

Investigation of the application of remote sensing technology to environmental monitoring

The objective of this project is the development of a new class of metalloporphyrin materials used as catalsyts for use in fuel cell applications. The metalloporphyrins are excellent candidates for use as catalysts at both the anode and cathode. The catalysts reduce oxygen in 1 M potassium hydroxide, as well as in 2 M sulfuric acid. Covalent attachment to carbon supports is being investigated. The computer-aided molecular design is an iterative process, in which experimental results feed back into the design of future catalysts.

1996-04-01

436

Invasiveness and persistence of Salmonella enteritidis, Salmonella typhimurium, and a genetically defined S. enteritidis aroA strain in young chickens.

Activities and results are reported of a project to investigate the application of remote sensing technology developed for the LACIE, AgRISTARS, Forestry and other NASA remote sensing projects for the environmental monitoring of strip mining, industrial pollution, and acid rain. Following a remote sensing workshop for EPA personnel, the EOD clustering algorithm CLASSY was selected for evaluation by EPA as a possible candidate technology. LANDSAT data acquired for a North Dakota test sight was clustered in order to compare CLASSY with other algorithms.

1980-08-01

437

GaInP/GaAs tandem concentrator cells

Newly hatched chicks were dosed orally with a Salmonella typhimurium wild-type strain, an S. enteritidis wild-type strain, and a genetically defined S. enteritidis aroA vaccine candidate, strain CVL30....Full Text Available

1994-11-01

438

Fibre optic grating sensors for biofuels

We discuss the initial development of a concentrator device based on the GaInP/GaAs monolithic tandem cell structure. The very high one-sun efficiency of this device, coupled with its characteristic low operating current, make this a promising candidate for use under high concentration. Test results for a prototype device are presented. This device achieves an efficiency of 29.5% at a concentration of 102 suns.

1994-06-30

439

Energy conservation via heat transfer enhancement. Quarterly progress report, October 1-December 31, 1979

Biofuels will have more intense impact on the energetic grid of the planet, because known fossil fuels reserves are being exhausted. The biofuel production relies on the transformation process of some organic material in the desired hydrocarbon product. Because of the natural characteristics of the related processes, fibre optic sensors appear to be adequate candidates to be used.

2010-09-01

440

Effect of environmental variables on localized corrosion of high-performance container materials

Contacts with researchers, manufacturers, and users of enhanced heat transfer equipment are summarized. The Technical Literature file and the Patent File continue to be updated; the Patent File is the source for a patent technology report now in draft form. Application studies on a rationally-based friction correlation for internally-finned tubes and on the evaluation of candidate tube-side enhancements for OTEC applications are reported. The technology study on enhancement of spray evaporation is also discussed.

1979-01-01

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441

Deep drawing and impact extrusion of magnesium alloys at room temperature

Electrochemical cyclic potentiodynamic polarization (CPP) experiments were performed on several candidate high-performance waste package container materials to evaluate their susceptibility to localized corrosion in aqueous environments relevant to the potential underground high-level nuclear waste repository. This paper presents the results of this study showing the effects of chloride ion (Cl) concentrations, pH, temperature, and electrochemical potential scan rate on the pitting corrosion behavior of these materials.

1997-01-01

442

Corrosion resistant coatings for silicon carbide heat exchanger tubes: Topical report

Magnesium alloys and composites are potentially highly attractive materials for lightweight design in the automotive industry - if common forming technologies, i.e., deep drawing and cold extrusion, are applicable to them; so far, this is seldom the case. The authors have tested some candidate Mg alloys and discuss the results of their measurements in detail. The Figure shows a typical workpiece part obtained by a deep-drawing technique. (Abstract Copyright [2003], Wiley Periodicals, Inc.)

2003-04-01

443

CNN Newsroom Classroom Guides, October 2000.

This heat exchanger is a critical step in the development of the Externally Fired Combined Cycle power system, a direct-coal combustion power plant (gas turbine). SiC is the only material with the needed resistance to creep, thermal shock, and oxidation; however a protective coating is needed. Ten candidate materials were identified: alumina-based materials, materials stable with SiO, and low expansion materials. An initial screening study should be performed.

1996-09-01

444

An X-ray source population study of the Andromeda galaxy M 31

These classroom guides, designed to accompany the daily CNN (Cable News Network) Newsroom broadcasts for the month of October 2000, provide program rundowns, suggestions for class activities and discussion, student handouts, and a list of related news terms. Top stories include: Chinese authorities detain Falun Gong protesters on Tiananmen Square and Pope John Paul II angers China by canonizing Chinese martyrs, U.S. presidential candidates prepare for the first presidential debate, U.S. presidential candidates debate education issues, Danville, Kentucky prepares for the first U.S. vice presidential debate, and Yugoslav protesters seize parliament (October 2-6); Yugoslavian president Vojislav Kostunica takes office, recent clashes between Arabs and Israelis concern neighboring Arab states, North Korea observes 55 years of communism with celebrations, Al Gore and George W. Bush meet in the second presidential debate, and Violence in the Mideast ...

1999-12-01

445

ANALYSIS OF CURRENT FIELD DATA

XMM-Newton EPIC observations reveal the population of X-ray sources of the bright Local Group spiral galaxy M 31, a low-star-formation-rate galaxy like the Milky Way, down to a 0.2-4.5 keV luminosity of 4.4E34 erg/s. With the help of X-ray hardness ratios and optical and radio information different source classes can be distinguished. The survey detected 856 sources in an area of 1.24 square degrees. Sources within M 31 are 44 supernova remnants (SNR) and candidates, 18 super-soft sources (SSS), 16 X-ray binaries (XRBs) and candidates, as well as 37 globular cluster sources (GlC) and candidates, i.e. most likely low mass XRBs within the GlC. 567 hard sources may either be XRBs or Crab-like SNRs in M 31 or background AGN. 22 sources are new SNR candidates in M 31 based on X-ray selection criteria. Time variability information can be used to improve the source classification. Two GlC sources show type I ...

2005-01-01

446

In vitro evaluation of herpes simplex virus type 1 thymidine kinase reporter system in dynamic studies of transcriptional gene regulation

This report provides a concise summary of the information collected and analyzed regarding the leak characteristics which define them as applicable candidates for pressure activated sealant technology. This information covers Office of Pipeline Safety reported incidents from 1985 to 1997 and was collected from existing data sources as well as operator and service company input.

2004-01-01

447

Wake-up-call, a lin-52 paralogue, and Always early, a lin-9 homologue physically interact, but have opposing functions in regulating testis-specific gene expression

The herpes simplex virus type 1 thymidine kinase (HSV1-TK) reporter system is being used to directly and indirectly monitor therapeutic gene expression, immune cell trafficking and protein-protein interactions in various living animals. However, the issues of HSV1-TK enzyme stability in living cells and whether this reporter system is optimal for dynamic studies of gene expression events in genetic imaging have not be addressed. The purpose of the present study was to evaluate the application of this reporter system in dynamic studies of transcriptional gene regulation. To achieve this purpose, we established two tetracycline-inducible murine sarcoma cell lines, tetracycline-turn-off HSV1-tk-expressing cell line (NG4TL4/tet-off-HSV1-tk) and tetracycline-turn-off Luc-expressing cell line (NG4TL4/tet-off-Luc), to create an artificially regulated gene expression model in vitro. The dynamic transcriptional ...

2006-07-15

448

Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung. Relationship between uteroglobin and eicosanoid levels in the developing fetal lung.

A conserved multi-subunit complex (MybMuvB, MMB), regulates transcriptional activity of many different target genes in Drosophila somatic cells. A paralogous complex, tMAC, controls...Full Text Available

2011-07-15

449

Transfer of Plasmid-Mediated CTX-M-9 from Salmonella enterica Serotype Virchow to Enterobacteriaceae in Human Flora-Associated Rats Treated with Cefixime?

Uteroglobin (UG) gene encodes a cytokine-like, multifunctional, antiinflammatory protein, with potent phospholipase A2-inhibitory activity. It has been suggested that during implantation this protein...Full Text Available

1995-07-01

450

Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter.

Food animals are a potential source of CTX-M resistance genes for humans. We evaluated the transfer of the bla_CTX-M-9 gene from an animal strain of Salmonella enterica...Full Text Available

2010-01-01

451

The effect of pyramiding Phytophthora infestans resistance genes R_Pi-mcd1 and R_Pi-ber in potato

The cell cycle-regulatory transcription factor E2F-1 is regulated by interactions with proteins such as the retinoblastoma gene product and by cell cycle-dependent alterations in E2F-1 mRNA abundance....Full Text Available

1994-10-01

452

The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors

Despite efforts to control late blight in potatoes by introducing R_pi-genes from wild species into cultivated potato, there are still concerns regarding...Full Text Available

2010-06-01

453

The Putative Natural Killer Decoy Early Gene m04 (gp34) of Murine Cytomegalovirus Encodes an Antigenic Peptide Recognized by Protective Antiviral CD8 T Cells

In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3′- and 5′-ends of 261 yeast genes by...Full Text Available

2010-08-01

454

TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.

Several early genes of murine cytomegalovirus (MCMV) encode proteins that mediate immune evasion by interference with the major histocompatibility complex class I (MHC-I) pathway of antigen presentation...Full Text Available

2000-02-01

455

Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU.

We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented...Full Text Available

1992-12-01

456

SWI/SNF and Asf1 Independently Promote Derepression of the DNA Damage Response Genes under Conditions of Replication Stress

The rates of synthesis of a class of both secreted and intracellular degradative enzymes in Bacillus subtilis are controlled by a signal transduction pathway defined by at least four regulatory genes:...Full Text Available

1990-02-01

457

Repetitive satellite-like sequences are present within or upstream from 3 avian protein-coding genes.

The histone chaperone Asf1 and the chromatin remodeler SWI/SNF have been separately implicated in derepression of the DNA damage response (DDR) genes in yeast cells treated with genotoxins that cause...Full Text Available

458

Regulation of expression of a soybean storage protein subunit gene. Progress report

Peculiar DNA sequences made up by the tandem repetition of a 5 bp unit have been identified within or upstream from three avian protein-coding genes. One sequence is located within an intron of the...Full Text Available

1983-03-11

459

Regulation of cell proliferation and apoptosis in neuroblastoma cells by ccp1, a FGF2 downstream gene

We have found that the methionine repression of the ..beta..-subunit gene expression is not due to degradation of the ..beta..-subunit but is due to an effect on synthesis of the ..beta..-subunit. The effect of methionine on the synthesis of the ..beta..-is due to an inhibition of ..beta..-subunit mRNA synthesis. 3 references, 1 figure.

1984-04-23

460

Quox-1, a quail homeobox gene expressed in the embryonic central nervous system, including the forebrain.

BackgroundCoiled-coil domain containing 115 (Ccdc115) or coiled coil protein-1 (ccp1) was previously identified as a downstream gene of Fibroblast Growth Factor 2 (FGF2) highly expressed...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Polygalacturonase ?-Subunit Antisense Gene Expression in Tomato Plants Leads to a Progressive Enhanced Wound Response and Necrosis in Leaves and Abscission of Developing Flowers

This paper reports the cloning and sequencing of a quail homeobox-containing gene, Quox-1, and its expression pattern in embryos from 3 to 6 days (E3 to E6) of development as determined by in situ hybridization....Full Text Available

1991-03-15

462

Physical and functional characterization of the gene cluster encoding the polyketide phytotoxin coronatine in Pseudomonas syringae pv. glycinea.

Tomato (Lycopersicon esculentum var. Better Boy) plants were transformed with a tomato leaf wound-inducible polygalacturonase (PG) β-subunit gene in the antisense orientation...Full Text Available

2003-10-01

463

Nucleotide sequence of a Euglena gracilis chloroplast gene coding for the 16S rRNA: homologies to E. coli and Zea mays chloroplast 16S rRNA.

Pseudomonas syringae pv. glycinea PG4180 produces the polyketide phytotoxin coronatine. The coronatine synthesis genes in PG4180 were previously shown to reside on a 90-kb plasmid designated p4180A....Full Text Available

1992-03-01

464

Molecular cloning of the N-terminus of GTBP

The nucleotide sequence of 16S rDNA from Euglena gracilis chloroplasts has been determined representing the first complete sequence of an algal chloroplast rRNA gene. The structural part of the 16S...Full Text Available

1982-10-25

465

Molecular characterization of cap3A, a gene from the operon required for the synthesis of the capsule of Streptococcus pneumoniae type 3: sequencing of mutations responsible for the unencapsulated phenotype and localization of the capsular cluster on the pneumococcal chromosome.

Defects in mismatch repair genes cause the genetic instability characteristic of hereditary nonpolyposis colorectal cancer and a subset of sporadic colon tumors. The newest member of the mismatch repair gene family, GTBP, has recently been identified as a partial cDNA. Here, we describe the isolation of its 5{prime} terminus, allowing definition of the entire coding region. Several polymorphisms within the 5{prime} end were identified and are presented. 13 refs., 1 fig.

1996-02-01

466

Metabolomic analysis of the plant pathogenic fungi Fusarium graminearum and Fusarium culmorum

The complete nucleotide sequence of the cap3A gene of Streptococcus pneumoniae, which is directly responsible for the transformation of some unencapsulated, serotype 3 mutants to the encapsulated phenotype,...Full Text Available

1994-10-01

467

Environmental Research Database

DescriptionThis project is part of the BBSRCs special initiative on plant and microbial metabolomics. The project will primarily focus on the trichothecene mycotoxin producing Ascomycete fungus Fusarium graminearum (Fg) which causes ear blight disease of small grain cereals. The project aims to explore the metabolome of various wild-type and single gene deletion Fg strains and to compare some of these with the identical gene mutation in the budding yeast, S. cerevisiae (Sc) and the saprophytic filamentous [continued...

2008-01-31

468

LOC387715/HTRA1 gene polymorphisms and susceptibility to age-related macular degeneration: A HuGE review and meta-analysis

Identification of physochemical factors controlling the capacity of nano-particles to penetrate cells of the respiratory epithelium

PurposeTo examine the association of age-related macular degeneration (AMD) with HtrA serine peptidase 1 (HTRA1) gene rs11200638 G→A polymorphism and LOC387715/...Full Text Available

469

Environmental Research Database

Objectives1) To establish a protocol for transfection of immortalised type I cells with SiRNA and its effect on cell viability; 2) To optimise silencing of genes involved in endocystosis using SiRNA and confirm successful transfection with western blotting; 3) To investigate the effect of gene silencing on uptake of flourescently-labelled latex nanoparticles.DescriptionTo investigate the mechanisms of particle uptake.

2009-01-31

470

Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae.

Human thrombomodulin gene is intron depleted: nucleic acid sequences of the cDNA and gene predict protein structure and suggest sites of regulatory control.

The sequence and cytological location of five Anopheles gambiae glutathione S-transferase (GST) genes are described. Three of these genes, aggst1-8, aggst1-9 and aggst1-10, belong to the insect class...Full Text Available

2001-10-15

471

Gene silencing induced by oxidative DNA base damage: association with local decrease of histone H4 acetylation in the promoter region

We have isolated a human thrombomodulin cDNA, and a human genomic clone containing the putative promoter domain, as well as the translated and untranslated regions of the endothelial cell receptor....Full Text Available

1987-09-01

472

Gene expression profiles deciphering rice phenotypic variation between Nipponbare (Japonica) and 93-11 (Indica) during oxidative stress.

Oxidized DNA bases, particularly 7,8-dihydro-8-oxoguanine (8-oxoG), are endogenously generated in cells, being a cause of carcinogenic mutations and possibly interfering with gene expression. We found...Full Text Available

2010-07-01

473

Frequency of conjugative transfer of plasmid-encoded ISEcp1 - bla_CTX-M-15and aac(6')-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon - role of transferases

Rice is a very important food staple that feeds more than half the world's population. Two major Asian cultivated rice (Oryza sativa L.) subspecies, japonica and indica, show significant phenotypic variation in their stress responses. However, the molecular mechanisms underlying this phenotypic variation are still largely unknown. A common link among different stresses is that they produce an oxidative burst and result in an increase of reactive oxygen species (ROS). In this study, methyl viologen (MV) as a ROS agent was applied to investigate the rice oxidative stress response. We observed that 93-11 (indica) seedlings exhibited leaf senescence with severe lesions under MV treatment compared to Nipponbare (japonica). Whole-genome microarray experiments were conducted, and 1,062 probe sets were identified with gene expression level polymorphisms between the two rice cultivars in addition to differential expression under MV treatment, which were assigned as Core ...

2010-01-08

474

Expression of bacterial luciferase in eukaryotic cells

BackgroundThe frequency of transfer of genes encoding resistance to antimicrobial agents was determined by conjugation in ESBL-producing and/or fluoroquinolone or aminoglycoside...Full Text Available

475

Expression of Genes Encoding F₁-ATPase Results in Uncoupling of Glycolysis from Biomass Production in Lactococcus lactis

Expression of Bacterial luciferase enzyme (lux) in mammalian cells would be a powerful bioreporter protein system for in vivo imaging because eukaryotic luciferases need expensive substrates. However, only a few efforts have been made to express bacterial luciferase enzyme in mammalian cells. As the result of this, we attempted to construct bicistronic vector including two bacterial luciferase genes (LuxA and LuxB) for assessing the potential to be visualized in vitro or in vivo by optical imaging system after transfection to mammalian cells. We designed and synthesized luxA and luxB genes from Photorhabdus Luminescens. To co-express both luxA and luxB genes from a single promoter, we cloned as a bicistronic transcript fused with an internal ribosomal entry site (IRES). This bicistronic transcript was transfected by Superfect to HEK 293T cell line. We also transfected lux A and lux B vector to HEK 293T cells separately. To ...

2005-11-18

476

Expression and fine structure of the gene encoding N epsilon-(indole-3-acetyl)-L-lysine synthetase from Pseudomonas savastanoi.

We studied how the introduction of an additional ATP-consuming reaction affects the metabolic fluxes in Lactococcus lactis. Genes encoding the hydrolytic part of the F₁ domain...Full Text Available

2002-09-01

477

Evolution of a cluster of innate immune genes (?-defensins) along the ancestral lines of chicken and zebra finch

The gene encoding N epsilon-(indole-3-acetyl)-L-lysine synthetase, iaaL, from Pseudomonas savastanoi was localized within a 4.25-kilobase EcoRI fragment derived from pIAA1 of oleander strain EW 2009....Full Text Available

1990-08-01

478

Enhanced anti-tumor effects of combined MDR1 RNA interference and human sodium/iodide symporter (NIS) radioiodine gene therapy using an adenoviral system in a colon cancer model

BackgroundAvian β-defensins (AvBDs) represent a group of innate immune genes with broad antimicrobial activity. Within the chicken genome, previous work identified 14 AvBDs...Full Text Available

479

Discordant expression and variable numbers of neighboring GGA- and GAA-rich triplet repeats in the 3' untranslated regions of two groups of messenger RNAs encoded by the rat polymeric immunoglobulin receptor gene.

Using an adenoviral system as a delivery mediator of therapeutic gene, we investigated the therapeutic effects of the use of combined MDR1 shRNA and human NIS (hNIS)...Full Text Available

2010-07-01

480

Cloning of rice DNA and identification of tRNA gene clones

An unusual S1-nuclease sensitive microsatellite (STMS) has been found in the single copy, rat polymeric immunoglobulin receptor gene (PIGR) terminal exon. In Fisher rats, elements within or beyond the...Full Text Available

1995-04-11

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481

Cambial-Region-Specific Expression of the Agrobacterium iaa Genes in Transgenic Aspen Visualized by a Linked uidA Reporter Gene1

DNA from 48 hr germinated rice embryos was cut with restriction endonuclease Bam H1 and cloned to the Bam H1 site on plasmid pBR 322. The clones containing recombinant DNA were selected by their sensitivity to tetracycline and resistance to ampicillin. Using /sup 32/P-labelled rice embryos tRNA as a probe two clones were identified to contain tRNA genes by colony hybridization.

1981-10-20

482

Application of a Newly Developed ARB Software-Integrated Tool for In Silico Terminal Restriction Fragment Length Polymorphism Analysis Reveals the Dominance of a Novel pmoA Cluster in a Forest Soil

The level of indole-3-acetic acid (IAA) was locally modified in cambial tissues of transgenic aspen (Populus tremula L. × Populus tremuloides Michx.). We also...Full Text Available

2000-06-01

483

Analysis of the murine All-1 gene reveals conserved domains with human ALL-1 and identifies a motif shared with DNA methyltransferases.

TRF-CUT, an ARB-implemented tool, was developed to predict in silico the terminal restriction fragments of aligned small-subunit rRNA gene or functional gene sequences. Application of this new tool...Full Text Available

2005-03-01

484

Analysis of nifH Gene Pool Complexity in Soil and Litter at a Douglas Fir Forest Site in the Oregon Cascade Mountain Range

A series of translocation break points found in a subset of human acute leukemias have one of the breaks on human chromosome 11q23. This region has recently been cloned and a large gene, ALL-1, with...Full Text Available

1993-07-01

485

An ALMT1 gene cluster controlling aluminium (aluminum) tolerance at the Alt4 locus of rye (Secale cereale L.)

Nitrogen-fixing microbial populations in a Douglas fir forest on the western slope of the Oregon Cascade Mountain Range were analyzed. The complexity of the nifH gene pool (nifH...Full Text Available

1999-02-01

486

A detailed comparison of the 5'-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA.

Aluminium toxicity is a major problem in agriculture worldwide. Among the cultivated triticeae, rye (Secale cereale L.) is one of the most Al-tolerant and represents an important potential source of Al-tolerance for improvement of wheat. The Alt4 Al-tolerance locus of rye contains a cluster of genes...

487

Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori

We have examined homologous fragments of DNA cloned from two different tissues for changes in the dNA sequence which might be related to tissue specific gene expression. The 5' end of the chicken ovalbumin...Full Text Available

1980-10-10

488

British Library Electronic Table of Contents (United Kingdom)

Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gus...

2011-01-01

489

INDUCIBLE RNAi-MEDIATED GENE SILENCING USING NANOSTRUCTURED GENE DELIVERY ARRAYS

Human tissue factor pathway inhibitor (TFPI) gene: Complete genomic structure and localization on the genetic map of chromosome 2q

RNA interference has become a powerful biological tool over the last decade. In this study, a tetracycline-inducible shRNA vector system was designed for silencing CFP expression and delivered alongside the yfp marker gene into Chinese hamster ovary cells using impalefection on spatially indexed vertically aligned carbon nanofiber arrays (VACNFs). The VACNF architecture provided simultaneous delivery of multiple genes, subsequent adherence and proliferation of interfaced cells, and repeated monitoring of single cells over time. Following impalefection and tetracycline induction, 53.1% 10.4% of impalefected cells were fully silenced by the inducible CFP-silencing shRNA vector. Additionally, efficient CFP-silencing was observed in single cells among a population of cells that remained CFP-expressing. This effective transient expression system enables rapid analysis of gene silencing effects using RNAi in single cells and cell ...

2008-01-01

490

Human cytoplasmic actin proteins are encoded by a multigene family

Tissue factor pathway inhibitor (TFPI), a protease inhibitor that circulates in association with plasma lipoproteins (VLDL, LDL and HDL), helps to regulate the extrinsic blood coagulation cascade. The authors have cloned a 125-kb genomic region containing the entire human TFPI gene on six overlapping cosmids and prepared a restriction map of this contig to clarify gene structure. More than half (45 kb) of the 85-kb gene is occupied with 5[prime] noncoding elements: coding begins at exon 3. A HindIII RFLP identified with one cosmid was genotyped in the CEPH panel of 559 reference families. Linkage analysis using markers on human chromosome 2 located the TFPI gene on 2q, 36 cM proximal to D2S43(pYNZ15) and 13 cM distal to the crystalline [gamma]-polypeptide locus CRYGP1(p5G1). 31 refs., 3 figs., 3 tabs.

1993-08-01

491

Final report of the specific research. Investigations on the analysis of bio-protective factors against radiation. 1998-2000 FY (Research Group of NIRS)

The authors characterized nine human actin genes that they isolated from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and ..cap alpha..-, ..beta..-, and ..gamma..-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria they show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5' and 3' ends of the coding region of a cloned chicken ...

1982-06-01

492

Expression profiles of PtrLOS2 encoding an enolase required for cold-responsive gene transcription from trifoliate orange

This report concerns investigations in the title conducted by 8 groups of National Institute of Radiological Sciences (NIRS) during the period of 1998-2000. The groups are for investigation of: Effects of p53 tumor suppressor gene in radiation-induced leukemia, Role of atm-gene in dose rate effect of ionizing radiation, Function of DNA-dependent protein kinase catalytic subunit (DNA-PK{sub cs}), Functional complementation of radiation-sensitive mutant M10 cell line by human XRCC4 cDNA expression, Role of radiation-induced apoptosis in digital defects in embryonic mice, Functional analysis of S-phase specific novel nuclear protein NP95 by gene targeting, Role of chemokine in T cell development and lymphomagenesis, and establishment of production techniques of gene-modified mice using embryonic stem cells for genetic analysis of radiation-sensitive genes. The groups describe summaries ...

2002-03-01

493

British Library Electronic Table of Contents (United Kingdom)

Low expression of osmotically responsive genes 2 (LOS2) encodes an enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) that converts 2-phospho-D-glycerate (PGA) to phosphoenolpyruvate (PEP) in the glycolytic pathway in Arabidopsis. Meanwhile, it is a transcriptional activator of cold-responsive gene, negatively controlling the expression of STZ/ZAT10, a zinc finger transcriptional repressor of cold-responsive gene from Arabidopsis. A novel LOS2 gene, designated PtrLOS2 (GenBank accession number GQ144341), was isolated from trifoliate orange [Poncirus trifoliata (L.) Raf.]. The PtrLOS2 cDNA is 1 662 bp in length with a 1 338 bp open reading frame (ORF), encoding a deduced 445 amino acid residue protein with a predicted molecular mass of 47.79 kDa and an isoelectric point of 5.54. The ded...

2011-01-01

494

Expression of fully functional tetrameric human hemoglobin in Escherichia coli

Copy number and orientation determine the susceptibility of a gene to silencing by nearby heterochromatin in Drosophila

Synthesis genes encoding the human #alpha#- and #beta#-globin polypeptides have been expressed from a single operon in Escherichia coli. The #alpha#- and #beta#-globin polypeptides associate into soluble tetramers, incorporate heme, and accumulate to >5% of the total cellular protein. Purified recombinant hemoglobin has the correct stoichiometry of #alpha#- and #beta#-globin chains and contains a full complement of heme. Each globin chain also contains an additional methionine as an extension to the amino terminus. The recombinant hemoglobin has a C_4 reversed-phase HPLC profile essentially identical to that of human hemoglobin A_0 and comigrates with hemoglobin A_0 on SDS/PAGE. The visible spectrum and oxygen affinity are similar to that of native human hemoglobin A_0. The authors have also expressed the #alpha#- and #beta#-globin genes separately and found that the expression of the #alpha#-globin gene alone results in ...

495

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

The classical phenomenon of position-effect variegation (PEV) is the mosaic expression that occurs when a chromosomal rearrangements moves a euchromatic gene near heterochromatin. A striking feature of this phenomenon is that genes far away from the junction with heterochromatin can be affected, as if the heterochromatic state {open_quotes}spreads.{close_quotes} We have investigated classical PEV of a Drosophila brown transgene affected by a heterochromatic junction {approximately} 60 kb away. PEV was enhanced when the transgene was locally duplicated using P transposase. Successive rounds of P transpose mutagenesis and phenotypic selection produced a series of PEV alleles with differences in phenotype that depended on transgene copy number and orientation. As for other examples of classical PEV, nearby heterochromatin was required for gene silencing. Modifications of classical PEV by alterations at a single site are ...

1996-02-01

496

Absence of linkage of apparently single gene mediated ADHD with the human syntenic region of the mouse mutant coloboma

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to ...

1988-10-01

497

Utilization of recently immobilized fertilizer nitrogen by wheat as influenced by some organic chemicals in a rice-wheat sequence

Attention deficit disorder (ADHD) is a complex biobehavioral phenotype which affects up to 8% of the general population and often impairs social, academic, and job performance. Its origins are heterogeneous, but a significant genetic component is suggested by family and twin studies. The murine strain, coloboma, displays a spontaneously hyperactive phenotype that is responsive to dextroamphetamine and has been proposed as a genetic model for ADHD. Coloboma is a semi-dominant mutation that is caused by a hemizygous deletion of the SNAP-25 and other genes on mouse chromosome 2q. To test the possibility that the human homolog of the mouse coloboma gene(s) could be responsible for ADHD, we have carried out linkage studies with polymorphic markers in the region syntenic to coloboma (20p11-p12). Five families in which the pattern of inheritance of ADHD appears to be autosomal dominant were studied. Segregation analysis of the traits studied suggested ...

1995-12-18

498

The socio-economic effects of the use of forest chips from logging residues and small-sized trees in energy production; case study; Pienpuuhakkeen ja hakkuutaehdehakkeen energiakaeytoen sosioekonomiset vaikutukset; case-tarkastelu -osaprojekti

The effectiveness of two organic chemicals, viz. 2, 4-dinitrophenyl hydrazone and naphthyl ethylene diamine applied at the rate of 10 mg kg"-"1 soil to wheat were studied as mobilizers of recently immobilized fertilizer N applied ("1"5N urea) at the rate of 60, 120 and 180 mg kg"-"1 soil to rice under greenhouse condition on a Typic Ustochrept. Rice as a first crop gave "1"5N recovery ranging from 35 to 37 per cent and more than one third of the applied fertilizer N was retained in the soil. Chemicals provided significantly higher mineral N during the initial growth period of wheat grown as a subsequent crop on the soil having different levels of residual nitrogen. "1'5N removal by plants at all the growth stages was significantly higher due to chemicals. The chemicals also showed significant effect in increasing the grain yield which caused higher N uptake and showed "1"5N recovery escalation. The "1"5N recovery at wheat harvest varied from 3 to 5 per cent of ...

499

Less waste corn, more land in soybeans, and the switch to genetically modified crops: trends with important implications to wildlife management

The aim of this case study is to analyse the socio-economic effects of forest fuel supply and use chains. Two of the four cases are rural district heating plants. In Perho, the co-operative organisation of forest owners maintains the fuel supply and operates the plant. In Ruukki, the local forestry association supplies forest chips to the plant. These medium-scale plants use small-sized trees from young thinning stands as their main fuel. The trees are felled manually. The other two cases are large power plants. Alholmens Kraft's new power plant in Pietarsaari aim to use 250 000 solid-m3 of forest chips annually. The logging residues are hauled to the plant as bales using traditional timber harvesting equipment or as bulk material. At young thinning stands the trees are harvested using an accumulating feller buncher. The Toppila I and II power plants in the city of Oulu used about 36 000 solid-m3 of forest chips. Over 300 000 solid-m3 ...

2003-07-01

500