WorldWideScience
1

Suppression of Allene Oxide Cyclase in Hairy Roots of Medicago truncatula Reduces Jasmonate Levels and the Degree of Mycorrhization with Glomus intraradices1[w  

UK PubMed Central (United Kingdom)

During the symbiotic interaction between Medicago truncatula and the arbuscular mycorrhizal (AM) fungus Glomus intraradices, an endogenous increase in jasmonic acid...Full Text Available

2005-11-01

2

Applied and fundamental aspects of plant cell, tissue, and organ culture  

CERN Document Server

Applied and fundamental aspects of plant cell, tissue, and organ culture

1977-01-01

3

Safety culture development at Daya Bay NPP  

International Nuclear Information System (INIS)

From view on Organization Behavior theory, the concept, development and affecting factors of safety culture are introduced. The focuses are on the establishment, development and management practice for safety culture at Daya Bay NPP. A strong safety culture, also demonstrated, has contributed greatly to improving performance at Daya Bay

2001-12-01

4

Hyaluronate synthesis by synovial villi in organ culture. [Dogs  

Energy Technology Data Exchange (ETDEWEB)

Individual canine synovial villi were used to establish short-term synovial organ cultures. These villi incorporated /sup 3/H-glucosamine into highly-polymerized /sup 3/H-hyaluronic acid (/sup 3/H-HA), which was the only /sup 3/H-glycosaminoglycan identified in the culture medium. Some /sup 3/H-HA, and larger amounts of other /sup 3/H-glycosaminoglycans, were recovered from cultured tissues. Culture medium /sup 3/H-HA content was proportional to the surface area of cultured villi. Organ cultures of nonvillous synovium were compared with villi; nonvillous cultures synthesized less /sup 3/H-HA per mm2 of their synovial intimal surface than villi. These cultures complement cell culture techniques for in vitro studies of synovial lining cell function.

1983-06-01

5

International workshop on 'in vitro' culture of forest tree species. Colloque international sur la culture 'in vitro' des essences forestieres  

Energy Technology Data Exchange (ETDEWEB)

Some 43 papers were presented in 4 sections: Organ and bud culture of tree species (24 papers); Cell suspension and callus culture of tree species (11); Culture of haploid tissue of tree species and production of wood homozygotes (3); and Isolation, culture and somatic fusion of protoplasts as a means of somatic hybridization and genetic engineering (5).

1983-01-01

6

Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture  

Energy Technology Data Exchange (ETDEWEB)

Micropropagation methods are described for the production of clonal lines from Eucalyptus marginata (jarrah) seedlings. Nodal explants from mature trees can also yield shoot cultures, but a high frequency of contamination occurs among such explants. Uncontaminated callus cultures can be produced from mature trees by culturing stamen filaments and shoots can subsequently be regenerated from this callus. The rooting percentage of shoot cultures from either nodes or stamen callus of mature trees is low compared with that from seedling explants. Considerable variation was observed between trees in the ability of stamen callus to regenerate shoots and in the frequency of rooting. (Refs. 27)

1982-01-01

7

Research, Preservation, and Education: An Introduction to Various Heritage Centers, Organizations, and Projects  

British Library Electronic Table of Contents (United Kingdom)

This forum showcases the work of a variety of different heritage-based centers, organizations, and projects dedicated to research, education, and preservation of tangible and intangible forms of cultural heritage. The descriptions of these centers demonstrate the diversity of heritage work being done today. The centers and projects described in the forum vary in their contexts, missions, and outcomes. Highlighted in the forum are preservation organizations, university-based heritage centers, and a global collaborative cultural heritage project. Each organization in the forum provides information about their missions and goals, their approaches or methods to heritage work, and a brief description of some of their initiatives.

2011-01-01

8

A structured approach to the assessment of the quality culture in nuclear installation  

International Nuclear Information System (INIS)

INSAG has emphasized that safety culture has two general components: the organizational framework and the attitude of the staff. To develop a structured approach to the assessment of safety culture, we propose that the highly formalized nature of nuclear power plant organizations be exploited. The prime coordinating mechanism of NPP organizations is the standardization of work processes, where a work process is defined as a standardized sequence of tasks designed to achieve a specific goal (an example is the maintenance work process). The predictable nature of work processes is exploited by the Work Process Analysis Model (WPAM) to conduct a systematic analysis that identifies the desirable characteristics of work processes and develops performance measures for their strengths and weaknesses. These can provide a set of tangible characteristics of a good safety culture. It is argued ...

1995-04-01

9

Organ culture of mammalian skin and the effects of ultraviolet light and testosterone on melanocyte morphology and function  

Science.gov (United States)

Scrotal skin of black Long-Evans rats and human thigh skin were maintained in vitro as organ cultures for as long as 14 days, and examined histologically using the combined skin splitting and Dopa techniques. Selected rat skin cultures received testosterone in the culture medium and/or were irradiated with ultraviolet light (290 to 320 nm uvl). With increased time in culture, scrotal melanocytes round up and there is an increase in epidermal pigmentation. Human skin behaves similarly; after eight days in vitro human melanocytes also become rounded, but remain strongly Dopa-positive. Addition of exogenous testosterone to cultured rat skin maintains dendritic morphology of melanocytes, but cell body size is still reduced. uvl irradiation stimulates melanocytes in rat skin cultures, maintaining their dendritic morphology and increasing ...

1978-05-01

10

Explant culture of gastrointestinal tissue: a review of methods and applications  

British Library Electronic Table of Contents (United Kingdom)

The gastrointestinal (GI) tract is an important target organ for the toxicity of xenobiotics. The toxic effects of xenobiotics on this complex, heterogeneous structure have been difficult to model in vitro and have traditionally been assessed in vivo. The explant culture of GI tissue offers an alternative approach. Historically, the organotypic culture of the GI tract proved far more challenging than the culture of other tissues, and it was not until the late 1960s that Browning and Trier described the means by which intestinal tissues could be successfully cultured. This breakthrough provided a tool researchers could utilise, and adapt, to investigate topics such as the pathogenesis of inflammatory intestinal diseases, the effect of growth factors and cytokines on intestinal proliferation...

2011-01-01

11

Characteristics of enriched cultures for bio-huff-`n`-puff tests at Jilin oil field  

Energy Technology Data Exchange (ETDEWEB)

Three enriched cultures (48, 15a, and 26a), selected from more than 80 soil and water samples, could grow anaerobically in the presence of crude oil at 30{degrees}C and could ferment molasses to gases and organic acids. Oil recovery by culture 48 in the laboratory model experiment was enhanced by 25.2% over the original reserves and by 53.7% over the residual reserves. Enriched culture 48 was composed of at least 4 species belonging to the genera Eubacterium, Fusobacterium, and Bacteroides. This enriched culture was used as inoculum for MEOR field trials at Jilin oil field with satisfactory results. The importance of the role of these isolates in EOR was confirmed by their presence and behavior in the fluids produced from the microbiologically treated reservoir.

1995-12-31

12

Pyogenic Spondylodiscitis after Percutaneous Endoscopic Lumbar Discectomy  

UK PubMed Central (United Kingdom)

We reviewed 9 cases of pyogenic spondylodiscitis following percutaneous endoscopic lumbar discectomy (PELD). Microbiologic cultures revealed 6 causative organisms. Five patients were managed conservatively...Full Text Available

2010-11-01

13

Association between the number of blood cultures and appropriateness of care for suspected bacteremic urinary tract infection in the elderly  

British Library Electronic Table of Contents (United Kingdom)

The objective of this study was to evaluate the association between the number of blood cultures collected and the appropriateness of care for suspected bacteremic community-acquired urinary tract infection (UTI) in the elderly. We retrospectively evaluated the medical records of 129 patients with UTI >65?years old admitted to a large community-based training hospital in Japan from 1 January 2006 to 31 December 2009. We assessed the association between the number of blood cultures collected and the appropriateness of care received, as well as other factors. Two-thirds of the patients were women, and patients >85?years old accounted for 45.0% of the cases. Most of the organisms isolated from the urine and blood were Escherichia coli (65.4?67.0%). More than two blood cultures were collected ...

2011-01-01

14

Identification of culturable and originally non-culturable endophytic bacteria isolated from shoot tip cultures of banana cv. Grand Naine  

British Library Electronic Table of Contents (United Kingdom)

In this article we describe the identification of endophytic bacteria belonging to three groups isolated from shoot tip cultures of banana cv. Grand Naine in a recent study (Thomas et?al. 2008) based on partial 16S rRNA gene sequence homology analysis. The first group included banana stocks that displayed obvious colony growth on MS based tissue culture medium during the first in?vitro passage. The second group constituted stocks that were tissue index-negative for cultivable bacteria initially but turned index-positive after a few to several (4?8) in?vitro passages while the third group formed one sub-stock that turned index-positive after about 18 passages. The organisms belonged to about 20 different genera comprising of ?, ?, ?-proteobacteria, Gram-positive firmicutes and actinobacteri...

2008-01-01

15

Uptake and translocation of {sup 137}Cs by Houttuynia cordata (in water culture)  

Energy Technology Data Exchange (ETDEWEB)

The water culture experiment of Houttuynia cordata of the medicinal plant was carried out, and basic study on {sup 137}Cs accumulation characteristic and internal circulation in H. cordata in the fruiting stage was investigated since the flowering season. H. cordata accumulated 80% of {sup 137}Cs absorbed unlike K to the root, rhizome, terminal bud of the underground part for the rhizome reproduction. {sup 137}Cs content to the young leaf, spike, involucre increased in the flowering season. Thereafter, {sup 137}Cs was recirculated to the developing organs in second generations such as the rhizome and bud. (author)

2001-08-01

16

Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture  

Energy Technology Data Exchange (ETDEWEB)

Methods are described for the production of callus and subsequent shoot and root induction and multiplication using stamen filaments, nodes of young stems of mature trees or cotyledon petioles. A high frequency of contamination occurred using nodal sections from mature trees, whereas up to 100% sterility was obtained with cotyledons and stamens. The rooting % of shoot cultures from nodes or stamen callus was low compared with that of shoots from seedling callus. Considerable variation was observed between clones from different trees in their ability to produce shoots and roots. 27 references

1982-01-01

17

Mechanism of biodegradation of paraquat by Lipomyces starkeyi  

Energy Technology Data Exchange (ETDEWEB)

The biodegradation of ring-/sup 14/C- and methyl-/sup 14/C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO/sub 2/. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

1985-05-01

18

Mechanism of biodegradation of paraquat by Lipomyces starkeyi  

International Nuclear Information System (INIS)

The biodegradation of ring-"1"4C- and methyl-"1"4C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO_2. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

19

Rapamycin (sirolimus) protects against hypoxic damage in primary heart cultures via Na^+/Ca^2^+ exchanger activation  

British Library Electronic Table of Contents (United Kingdom)

Aims: Rapamycin (sirolimus) is an antibiotic that inhibits protein synthesis through mammalian targeting of rapamycin (mTOR) signaling, and is used as an immunosuppressant in the treatment of organ rejection in transplant recipients. Rapamycin confers preconditioning-like protection against ischemic-reperfusion injury in isolated mouse heart cultures. Our aim was to further define the role of rapamycin in intracellular Ca^2^+ homeostasis and to investigate the mechanism by which rapamycin protects cardiomyocytes from hypoxic damage. Main methods: We demonstrate here that rapamycin protects rat heart cultures from hypoxic-reoxygenation (H/R) damage, as revealed by assays of lactate dehydrogenase (LDH) and creatine kinase (CK) leakage to the medium, by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-d...

2011-01-01

20

Site of lupanine and sparteine biosynthesis in intact plants and in vitro organ cultures  

International Nuclear Information System (INIS)

["1"4C]Cadaverine was applied to leaves of Lupinus polyphyllus, L. albus, L. angustifolius, L. perennis, L. mutabilis, L. pubescens, and L. hartwegii and it was preferentially incorporated into lupanine. In Lupinus arboreus sparteine was the main labelled alkaloid, in L. hispanicus it was lupanine. A pulse chase experiment with L. angustifolius and L. arboreus showed that the incorporation of cadaverine into lupanine and sparteine was transient with a maximum between 8 and 20 h. Only leaflets and chlorophyllous petioles showed active alkaloid biosynthesis, whereas no incorporation of cadaverine into lupanine was observed in roots. Using in vitro organ cultures of Lupinus polyphyllus, L. succulentus, L. subcarnosus, Cytisus scoparius and Laburnum anagyroides the inactivity of roots was confirmed. Therefore, the green aerial parts are the major site of alkaloid biosynthesis in lupins and in other legumes. (orig.).

1987-01-01

21

Differential immunotoxic effects of inorganic and organic mercury species in vitro  

British Library Electronic Table of Contents (United Kingdom)

Despite the fact that humans are exposed to multiple forms of mercury (elemental, inorganic, and organic), most research on mercury toxicity has focused on methylmercury (MeHg) and on neurotoxic outcomes and mechanisms. Recent work has indicated that the immunotoxic effects of mercury compounds may be significant contributors to human disease as well as mechanistically relevant to other target organ toxicities. In this study, we compared the effects of inorganic Hg (iHg) to organic Hg species (MeHg and ethylmercury, EtHg) in human peripheral blood mononuclear cells (PBMCs) in vitro at sub-cytotoxic concentrations, using methods developed to characterize response of human PBMCs to iHg in vitro. PBMCs were isolated from six volunteer blood donors (three males and three females) and cultured ...

2010-01-01

22

Fear, panic and prosperity consciousness: developing resilience as the paradigm shifts  

British Library Electronic Table of Contents (United Kingdom)

Purpose - The article shares insights from a program that was developed to address burnout, compassion fatigue and vicarious trauma. The program works from the premise that people and organizations thrive when they have shared values and common understandings. Design/methodology/approach - The approach draws together reflection at the organizational and person levels. Connecting business ethics and organizational analysis, improving understanding of the body's energy as well as inter-cultural understanding are all achievable while adopting the resilience development methods. Findings - The article concludes that reflective practice through the tools used on the program enhances understanding of the challenges faced by organizations and the individuals involved. It provides creative routes ...

2009-01-01

23

Method for increasing the pressure in oil-bearing geological structures  

Energy Technology Data Exchange (ETDEWEB)

A method is described for increasing pressure in geologic oil-bearing structures by gas production due to microbial activity. There is added to an oil-bearing structure an anaerobic micro-organism (Methanobacterium termoautotroficum), a culture medium and formic acid. The formic acid is converted to carbon dioxide and methane gases resulting in increased pressure in the structures. Methanobacterium termoautotroficum cannot split formic acid into CO/sub 2/ and H/sub 2/ but it can produce methane from these 2 gases. 4 claims.

1981-11-17

24

Convergenomics Strategic Innovation in the Convergence Era  

CERN Document Server

Convergenomics is about the megatrends that are shaping how people behave and organizations work. In this insightful analysis, Sang Lee and David Olson describe how globalization, digitization, changing demographics, changing industry mix, deregulation and privatization, commoditization of processes, new value chains, emerging new economies, deteriorating environment, and cultural conflicts have led to what they define as a convergence revolution.Lee and Olson discuss this convergence revolution from the perspectives of technology, industry, knowledge, open-source networking and bio-artificial

2010-01-01

25

Adherence of Enterohemorrhagic Escherichia coli O157, O26, and O111 Strains to Bovine Intestinal Explants Ex Vivo?  

UK PubMed Central (United Kingdom)

We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion...Full Text Available

2007-05-01

26

The efficacy of simulated solar disinfection (SODIS) against Ascaris, Giardia, Acanthamoeba, Naegleria, Entamoeba and Cryptosporidium  

British Library Electronic Table of Contents (United Kingdom)

The antimicrobial activity of simulated solar disinfection (SODIS) in the presence and absence of riboflavin against various protozoa and helminth organisms was investigated in this study. Assays were conducted in transparent 12 well microtitre plates containing a suspension of test organisms in the presence or absence of 250mM riboflavin. Plates were exposed to simulated sunlight at an optical irradiance of 550Wm^-^2 (watts per square metre) delivered from a SUNTEST(TM) CPS+ solar simulator. Aliquots of the test suspensions were taken at set time points and the viability of the test organisms was determined by either culture, microscopy or flow cytometry where applicable. With Acanthamoeba, Naegleria, Entamoeba and Giardia exposure to SODIS at an optical irradiance of 550Wm^-^2 for up to ...

2011-01-01

27

Enhanced In Vivo Function of Bioartificial Lungs in Rats  

British Library Electronic Table of Contents (United Kingdom)

BackgroundMore than 11 million Americans live with chronic lung disease; in search for an alternative to donor organs, we attempted to regenerate lungs based on perfusion decellularized lung scaffolds that can be transplanted similar to a donor organ. MethodsCadaveric rat lungs were decellularized by detergent perfusion. Resulting scaffolds were mounted in bioreactors and seeded with endothelial and fetal lung cells. Biomimetic organ culture was maintained for 7 days. Resulting bioartificial left lungs were transplanted in orthotopic position after left pneumonectomy in rats. Cadaveric left lung transplants and pneumonectomies served as controls. Blood gas analyses, compliance testing, and fluoroscopies were performed on postoperative days 1, 7, and 14. Lungs were removed for final analysi...

2011-01-01

28

Safety evaluation of Propionibacterium freudenreichii ET-3 culture.  

Science.gov (United States)

Propionibacterium freudenreichii ET-3 culture, a cell-free product of whey fermentation using P. freudenreichii ET-3 (7025), has been shown to promote the growth of Bifidobacteria through the action of 1,4-dihydroxy-2-naphthoic acid (DHNA), and therefore, has potential use in the food and supplement industries. Although currently used as a food ingredient in Japan, the safety of this novel ingredient has not been previously evaluated through traditional toxicity testing. Therefore, here we report the results of standard toxicological testing performed on P. freudenreichii ET-3 culture. In a 4-week oral toxicity study, administration of 6000mg/kg body weight/day P. freudenreichii ET-3 culture was without compound-related adverse effects on clinical signs, body weights, food consumption, ophthalmology, hematology, clinical chemistry, urinalysis, organ weights, and gross and microscopic findings in male ...

2011-03-22

30

Screening for marine nanoplanktic microalgae from Greek coastal lagoons (Ionian Sea) for use in mariculture  

British Library Electronic Table of Contents (United Kingdom)

Mediterranean mariculture uses imported strains of marine phytoplankton, raising questions of ecological risk and ability to adapt to local conditions for mass culture outdoors. In this context, we report here on the mass-culture potential and chemical composition of six strains of Prasinophyceae (five strains of Tetraselmis sp. and one Pyramimonas sp.) isolated from a Greek coastal lagoon. Proximate composition had a pattern of 10?20% ash, 35?65% protein, 6?10% lipids, and 25?45% other organics including carbohydrates. The amino acid profiles were typical for the marine representatives of the class. All strains had a high PUFA content with dominant the ?3 fraction in four of them. The fatty acid profiles indicated a Tetraselmis strain with high EPA (14%) and a Pyramimonas strain with high...

2009-01-01

31

Responses of the toxic cyanobacterium Microcystis aeruginosa to iron and humic substances  

British Library Electronic Table of Contents (United Kingdom)

Iron is an essential element to marine biota. Different types of dissolved organic matter (DOM), such as humic substances have impacts on the marine coastal waters iron chemistry.The aim of the study was to examine how the presence of humic substances (both aquatic and sedimentary) may affect iron bioavailability to the bloom-forming cyanobacterium Microcystis aeruginosa Kutzing incubated on standard and modified mineral BG-11 media. The final iron concentrations in the growth media ranged from 0.1 to 100mM.The results demonstrate that both the growth rate and the concentration of chlorophyll a in cultures of M. aeruginosa are limited by insufficient (a 15-fold as compared with the cultures exposed to iron only at the same concentration. Sedimentary humic acids in the presence of iron at a...

2007-01-01

32

Influenza Vaccines  

International Science & Technology Center (ISTC)

Development of the Culture Influenza Vaccine

33

Use of nuclear techniques in studies of uptake and metabolic fate of xenobiotics in plants  

International Nuclear Information System (INIS)

The use of municipal sewage sludge as a fertilizer in agriculture is a convenient method of disposal. However, sludge is often contaminated with toxic organic compounds such as dioxins, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs), with implications for soil fertility and quality of crops for human and animal consumption. These compounds can be assimilated by intact plants or in-vitro cell-Culture systems. The amount of uptake depends on the plant species and on the physico-chemical conditions that influence, for example, molecular configuration; uptake rates are higher with low-molecular-weight and polar compounds. The xenobiotic can be converted to polar conjugates and hydroxylated metabolites that may also be toxic. In some cases, large amounts of the compound and/or its metabolic products are incorporated into non-extractable residues. The bound residues, especially those associated with carbohydrate ...

1997-10-01

34

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay  

Energy Technology Data Exchange (ETDEWEB)

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis lucida, hepatoma-derived cell cultures ...

1994-08-01

36

Tissue structure, nuclear organization and gene expression in normal and malignant breast  

Energy Technology Data Exchange (ETDEWEB)

Because every cell within the body has the same genetic information, a significant problem in biology is to understand how cells within a tissue express genes selectively. A sophisticated network of physical and biochemical signals converge in a highly orchestrated manner to bring about the exquisite regulation that governs gene expression in diverse tissues. Thus, the ultimate decision of a cell to proliferate, express tissue-specific genes, or apoptose must be a coordinated response to its adhesive, growth factor, and hormonal milieu. The unifying hypothesis examined in this overview is that the unit of function in higher organisms is neither the genome nor the cell alone but the complex, three-dimensional tissue. This is because there are bidirectional connections between the components of the cellular microenvironment (growth factors, hormones, and extracellular matrix) and the nucl2048 These connections are made via membrane-bound receptors and transmitted to ...

2000-01-27

37

Experimental models for testing respiratory tract carcinogens  

Energy Technology Data Exchange (ETDEWEB)

The usefulness of the mouse, rat, and Syrian golden hamster as experimental models for studies of the development of respiratory tract cancer is considered. Three different application methods for examining the effects of environmental chemicals on the respiratory tract in these species are discussed: implantation, intratracheal instillation, and inhalation. The results of various investigations are presented to demonstrate that each particular method has both advantages and disadvantages. The techniques are also considered in comparison with the human situation and in the light of obtaining the most valid data for extrapolation to man. Brief attention is paid to the relevance to respiratory tract carcinogenesis of more recently developed organ and cell culture systems.

1982-01-01

38

Education with ICT in South Korea and Chile  

British Library Electronic Table of Contents (United Kingdom)

This article presents a linear-analytical case study on the development of ICT within the educational systems of Chile and South Korea. Through a comprehensive meta-data analysis and bibliographic review, we collected information on both educational systems and their ICT adoption policies. Key differences necessary to understand how both countries have developed their educational systems by integrating ICT were analyzed, including the educational system structure, the organization of state entities responsible for educational ICT, cultural characteristics, the creation of policies regarding ICT in education, and the effectiveness of such policies for the expansion of infrastructure and the ICT curriculum integration. We analyze these key differences in order to understand two cases of ICT ...

2011-01-01

39

Inhibition of heat shock protein 27 (HspB1) tumorigenic functions by peptide aptamers  

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

2011-01-01

40

Environmental education work force pipeline strategic plan  

Energy Technology Data Exchange (ETDEWEB)

This document describes an educational program designed to provide a pool of highly qualified administrative, technical, and managerial graduates that are familiar with the Hanford Site and business operations. The program is designed to provide work experience and mentoring to a culturally diverse student base which enhances affirmative employment goals. Short-term and long-term objectives of the program are outlined in the report, and current objectives are discussed in more detail. Goals to be completed by the year 2003 are aimed at defining the criteria necessary to establish partnerships between schools, community organizations, and human resources departments. Actions to be implemented includes providing instructors and equipment, enhancing skills of local teachers, and establishing collaboration with human resources organizations. Long-term goals of the program are to ensure a constant supply of qualified, trained ...

1992-11-01

41

Science Fiction as Social Movement: Ideology and Resource Mobilization in Cultural Production and Reproduction.  

Science.gov (United States)

The paper examines science fiction literature as a product and part of the social consciousness of the modern capitalist world order. This world order is seen as emphasizing science, technology, movement, growth, urbanization, industrialization, complex organization, and progress. The document is organized into two sections. The first section reviews theories of the sociology of knowledge and discusses knowledge as a process of self-production and reproduction of society. Influential theorists include Hegel, Marx, Lukacs, Mannheim, Gramsci, Habermas, and Touraine. Touraine's (1977) dynamic conflict-based systematic theory of society is based on three concepts: historicity, which defines the instruments of society's self-production; the system of historical action which is the totality of social and cultural orientations, by means of which historicity exercises its control of historicity and the system of historical action. ...

1980-10-01

42

Effects of ischemic-like insult on myocardial /sup 201/Tl accumulation  

Energy Technology Data Exchange (ETDEWEB)

Despite extensive clinical use of thallium-201 (/sup 201/Tl) for myocardial imaging, the effect of ischemia on myocardial accumulation and release of /sup 201/Tl independent of flow has not been fully defined. Therefore, myocardial accumulation of /sup 201/Tl in response to ischemic-like myocardial injury was assessed in vitro using the cultured fetal mouse heart preparation. Cultured fetal mouse hearts (n . 311) were subjected to injury simulating ischemia by deprivation of oxygen and oxidizable substrates for periods ranging from 15 minutes to 10 hours. The extent of irreversible injury was determined by the percentage of lactic dehydrogenase (LDH) lost from the hearts to the culture medium during recovery from injury. Injury was essentially reversible at 1 hour of insult. The fraction of /sup 201/Tl content in injured compared with control hearts was not significantly lower after 1 hour of insult. By 3 hours of insult, ...

1983-04-01

43

A study of bacterial isolates from corneal specimens and their antibiotic resistance profile  

International Nuclear Information System (INIS)

We aim to examine the spectrum of bacteria causing corneal infections and their antibiotic susceptibility patterns. This will serve as a guideline for empiric therapy of corneal infections. We conducted the study over a period of 18 months from March 2001 through December 2002 in King Abdul-Aziz University Hospital, Riyadh, Kingdom of Saudi Arabia. Corneal specimens taken from 200 patients were inoculated directly onto different types of media. The isolates were identified and then tested against the appropriate topical or systemic antibiotics. Sixty-seven (33.5%) of the total specimens were culture positive and 133 (66.5%) were culture negative. Fourteen (7%) of these showed organisms in the Gram stained smears and correlated well with the culture reports. Of the 67 positive cultures, 53 (79.1%) were Gram-positive bacteria mostly coagulase-negative Staphylococci 29 (43.3%) followed ...

44

Cross-Cultural Studies of Personality Traits and their Relevance to Psychiatry  

UK PubMed Central (United Kingdom)

AimsThis article provides a brief review of recent cross-cultural research on personality traits at both individual and culture levels, highlighting the relevance...Full Text Available

2006-01-01

45

Geographic Response Information Network : a new tool to manage community information for oil spill contingency planning and response operations  

Energy Technology Data Exchange (ETDEWEB)

This paper described the Geographic Response Information Network (GRIN) project which was launched to address some of the logistical challenges that often complicate oil spill and emergency response operations. The objective of the project was to develop a computer-based tool for incident logistics to organize maps and data related to oil spills, safety, public relations and basic community resources. In addition to its use for oil spill response planning, the data available can be useful for all-hazards emergency response planning. Early prototypes of the GRIN used PowerPoint slides to organize basic information about coastal communities in Alaska. With time, hyper text markup language (html) was used as the programming format because it can be more readily hyper-linked. Currently, GRIN is organized as a web page with the following 5 categories of information: general, liaison, public information, logistics and safety. ...

2006-07-01

46

Competency build up, sustained performance enhancement of human resource through effective man power planning, training and proper safety culture and organizational climate  

International Nuclear Information System (INIS)

Human resource competence building and continued enhancement of performance is the most vital input for safe and reliable operations of a Nuclear Power Plant. Integrated planning leading to the decision of timely selection of optimum number of fresh people and deployment of experienced manpower with desired lead time is inevitable to achieve the above objective. For safe and reliable plant operation human performance analysis followed by suggestive measures to improve the same is needed. Corrective or strengthening input may be in terms of training, work environment, motivations, organizational culture and climate, leadership and prevailing environmental force and bio-rhythm of individuals with critical days are to be worked out The adequacy in training and development not only gives safe and reliable plant operations but results in greater employee satisfaction and self esteem as well. As of date, in the present vibrant global scenario, only the ...

2006-11-13

47

Age sensitivity of juvenile mussels (Utterbackia imbeciles Say) to copper and cadmium exposure  

Energy Technology Data Exchange (ETDEWEB)

In recent years, there has been increased interest in using early life stages of freshwater bivalves to test the toxicity of dissolved constituents in water. The authors have developed laboratory and in situ assays with artificially cultured Utterbackia imbecillis to examine effects of contaminants and existing conditions in embayments and rivers on mortality and reproductive success of unionid mussels. Age sensitivity of U. imbecillis to Cd and Cu was examined using both static acute and 8-day static renewal bioassays. Both aqueous and sediment exposures demonstrated greater sensitivity to Cd than Cu. LC{sub 50}`s for two-day old (2d) and 9d mussels were approximately half the LC{sub 50} calculated for 16d mussels in 48-hr bioassays with Cd. Additionally, acute assays were repeated twice to examine variability in response between different mussel cultures to the two metals. The results of this work reinforce the potential of juvenile U. ...

1994-12-31

48

Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens-Mediated Transformation of Hybrid Aspen and Poplar Clones  

UK PubMed Central (United Kingdom)

Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures...Full Text Available

1990-07-01

50

A study on multi-cultural family wives adapting to Korean cuisine and dietary patterns  

UK PubMed Central (United Kingdom)

With the increase in multi-cultural families, Korea is seeing a rapid increase in immigrated housewives, who are closely related to food culture. However, studies for the diet of multi-cultural families,...Full Text Available

2010-10-01

52

Technical support for nuclear regulatory activity and the Italian experience  

International Nuclear Information System (INIS)

Different cases exist in various countries regarding the institutional status, the organization and the technical resources of the Nuclear Regulatory Authority (NRA): there are NRAs having extended internal technical capability and others relying mostly on external technical support. Considering the research and development activities and the very broad range of technical matters on which the regulatory and licensing activity are based, the NRA can not rely only on internal resources, and generally there is the need of technical and scientific support together with enhanced international cooperation. The international cooperation and networking among NRAs, and their Technical Support Organizations (TSO), represents a fundamental way to maintain competence, capability and knowledge. Within the EU member states this is becoming more and more an institutional duty. In providing technical support for regulatory activity, the experience shows, from ...

2007-08-01

53

University of Central Lancashire - Facilities  

Wastenet

...specialised laboratories for pharmaceutics, tissue culture and molecular biology Excellent Laboratory Facilities Tissue Culture Facilities Molecular Biology Laboratory ...

56

Indicators of safety culture - selection and utilization of leading safety performance indicators  

International Nuclear Information System (INIS)

Safety indicators play a role in providing information on organizational performance, motivating people to work on safety and increasing organizational potential for safety. The aim of this report is to provide an overview on leading safety indicators in the domain of nuclear safety. The report explains the distinction between lead and lag indicators and proposes a framework of three types of safety performance indicators - feedback, monitor and drive indicators. Finally the report provides guidance for nuclear energy organizations for selecting and interpreting safety indicators. It proposes the use of safety culture as a leading safety performance indicator and offers an example list of potential indicators in all three categories. The report concludes that monitor and drive indicators are so called lead indicators. Drive indicators are chosen priority areas of organizational safety activity. They are based on the underlying safety model and ...

2010-05-01

57

The Art of Articulation: Political Engagement and Social Movements in the Making among Young Adults in Multicultural Settings in Norway  

British Library Electronic Table of Contents (United Kingdom)

Participation in conventional politics, such as elections, membership of organizations and political parties, is relatively low among young adults of ethnic minority background. Instead, engagement seems to find its way through aesthetic and other expressive channels of influence drawing on new technologies, impulses from transnational youth culture traditions, and both street riots and less conflictual actions. The aim of this article is to grasp a potential social movement in the making, by exploring the processes of articulation through which young people from immigrant families in Norway express their political engagement. The phenomenon of articulation is understood as a process comprising three steps or modes: (i) becoming conscious; (ii) generating expressions and (iii) collective a...

2011-01-01

58

The ?Non-cuttable? Space in Between: Context, Boundaries and Their Natural Fluidity  

British Library Electronic Table of Contents (United Kingdom)

The inherent open-ended nature of socio-cultural phenomena makes it necessary to create new theoretical frameworks that enable us to understand the fluidity of the relations between different parts of the dynamic system. Recent advance in modern biology seems to provide a set of heuristic concepts useful to investigate the complexity and fluidity of the relationship between different contexts. One of these is the Rayner?s logic of natural inclusionality. According whit Rayner?s evolutionary standpoint any living system is constantly in dialogue with its natural neighbourhood on the basis of an interdependent and co-evolutive process involved both the context and the organism. The aim of this paper is to evaluate the model proposed by Rayner in light of contextual perspective in development...

2011-01-01

59

Searching for immunomodulatory sequences in sea bass (Dicentrarchus labrax L.): Transcripts analysis from thymus  

British Library Electronic Table of Contents (United Kingdom)

The thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cDNA library from sea bass (Dicentrarchus labrax), one of the most extensively cultured species in South Mediterranean aquaculture. A total of 1632 ESTs from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ESTs were further processed. The total collection of D. labrax thymus ESTs has been deposited in the EBI-GenBank-DBJ database (GenBank accession numbers from FN565576 to FN566839). The functional classification of ESTs was performed by Gene Ontology and KEGG annotation and, successively, the sequences were analysed using the Immuno...

2010-01-01

60

Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy  

CERN Document Server

To understand dynamic developmental processes, living tissues must be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in indeterminately growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image at cellular resolution a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, track cellular nuclei and identify cell divisions. We demonstrate the system's capabilities by collecting data on ...

2011-01-01

61

Natural plant chemicals: source of industrial and medicinal materials  

Energy Technology Data Exchange (ETDEWEB)

Many higher plants produce economically important organic compounds such as oils, resins, tannins, natural rubber, gums, waxes, dyes, flavors and fragrances, pharmaceuticals, and pesticides. However, most species of higher plants have never been described, much less surveyed for chemical or biologically active constituents, and new sources of commercially valuable materials remain to be discovered. Advances in biotechnology, particularly methods for culturing plants cells and tissues, should provide new means for the commercial processing of even rare plants and the chemicals they produce. These new technologies will extend and enhance the usefulness of plants as renewable resources of valuable chemicals. In the future, biologically active plant-derived chemicals can be expected to play an increasingly significant role in the commercial development of new products for regulating plant growth and for insect and weed control. 65 references.

1985-01-01

62

Managing between science and industry: An historical analysis of the Philips Research and Development Department's management  

British Library Electronic Table of Contents (United Kingdom)

Purpose - This paper seeks to deal with the history of Research and Development (R&D) management. It takes the history of the R&D Department of the Royal Philips Electronics of The Netherlands as an example to unravel the dynamics behind industrial R&D management. Design/methodology/approach - This paper is based upon historical and theoretical studies on industrial R&D institutions and research cultures. Findings - The paper proposes that the directors of the Philips R&D Department continually shaped and reshaped the organization in order to retain researchers with creative ideas, and to stimulate innovativeness. The R&D-management was the outcome of a search process that comprehended a mixture of scientific and industrial (management) skills, knowledge and experti...

2007-01-01

63

How to organize a neutron imaging user lab? 13 years of experience at PSI, CH  

British Library Electronic Table of Contents (United Kingdom)

PSI has a relatively long tradition in neutron imaging since the first trials were done at its formerly existing research reactor SAPHIR with film methods. This reactor source was replaced after its shutdown in 1994 by the spallation neutron source SINQ in 1996, driven by the 590MeV cyclotron for protons with presently up to 2.3mA beam current. One of the first experimental devices at SINQ was the thermal neutron imaging facility NEUTRA, which was designed from scratch and has been the first device of its kind at a spallation source. Until now, NEUTRA has been successfully in use for many investigations in a wide range of studies covering fuel cell research, environmental behavior of plants, nuclear fuel inspection and the research on cultural heritage objects. It has been the host of PhD ...

2011-01-01

64

Educational policymaking in the Tanzanian postcolony: authenticity, accountability, and the politics of culture  

British Library Electronic Table of Contents (United Kingdom)

In this article I explore the political event of the Tanzanian government's interdiction of a prominent educational civil society organization in order to theorize the emerging policy heterarchy in Tanzania. In the context of the ensuing public debate about the interdiction, I ask two questions: (1) what tensions are emerging from the democratization reforms embedded in national iterations of international EFA policies and (2) to what end are such reforms taking place? I address these questions with ethnographic, documentary and interview data collected as part of a broader study of participation in development between 2004 and 2007 in Dar es Salaam and the Singida region of central Tanzania. My analysis draws on critical, anthropological and postcolonial theories to explore the tensions o...

2011-01-01

65

Borrelia Burgdorferi ''sensu lato'' in Brazil: Occurrence confirmed by immunohistochemistry and focus floating microscopy  

British Library Electronic Table of Contents (United Kingdom)

In the present study, we report the occurrence of Lyme's borreliosis in patients from the Brazilian Amazon Region. Borreliosis was investigated by immunohistochemistry and focus floating microscopy for Borrelia burgdorferi in skin biopsy samples from 22 patients with both clinical and histopathology evidences compatible with Erythema Migrans. Spirochetes were detected by specific immunohistochemistry and focus floating microscopy for B. burgdorferi in samples from five patients. Clinical cure of the cutaneous lesions was observed in all the patients after treatment with doxycycline regimen as proposed by the Center Disease Control guidelines. A limitation of our study was the fact that we were not able to isolate and culture these organisms. These are the first known Brazilian cases of bor...

2010-01-01

66

The thorny path linking cellular senescence to organismalaging  

Energy Technology Data Exchange (ETDEWEB)

Half a century is fast approaching since Hayflick and colleagues formally described the limited ability of normal human cells to proliferate in culture (Hayflick and Moorhead, 1961). This finding--that normal somatic cells, in contrast to cancer cells, cannot divide indefinitely--challenged the prevailing idea that cells from mortal multicellular organisms were intrinsically ''immortal'' (Carrell, 1912). It also spawned two hypotheses, essential elements of which persist today. The first held that the restricted proliferation of normal cells, now termed cellular senescence, suppresses cancer (Hayflick, 1965; Sager, 1991; Campisi, 2001). The second hypothesis, as explained in the article by Lorenzini et al., suggested that the limited proliferation of cells in culture recapitulated aspects of organismal aging (Hayflick, 1965; Martin, 1993). How well have these hypotheses weathered the ...

2005-08-09

67

Protein-coding and non-coding gene expression analysis in differentiating human keratinocytes using a three-dimensional epidermal equivalent.  

Science.gov (United States)

The epidermal compartment is complex and organized into several strata composed of keratinocytes (KCs), including basal, spinous, granular, and cornified layers. The continuous process of self-renewal and barrier formation is dependent on a homeostatic balance achieved amongst KCs involving proliferation, differentiation, and cell death. To determine genes responsible for initiating and maintaining a cornified epidermis, organotypic cultures comprised entirely of stratified KCs creating epidermal equivalents (EE) were raised from a submerged state to an air/liquid (A/L) interface. Compared to the array profile of submerged cultures containing KCs predominantly in a proliferative (relatively undifferentiated) state, EEs raised to an A/L interface displayed a remarkably consistent and distinct profile of mRNAs. Cultures lifted to an A/L interface triggered the induction of gene groups that regulate ...

2010-05-25

68

Determination of a various ions such as alkali metals in leaves, stems, roots and seeds of the radish and their distribution  

Energy Technology Data Exchange (ETDEWEB)

Determination, uptake and distribution of various ions such as alkali metals in three different parts (leaf, stem and root) and seeds of radish (Kaiware daikon) were examined using flame emission spectrometry and ICP-AES. In order to examine the influence of concentration alkali metal ion concentration in the radish culture solution on the uptake and distribution of these metals, the radish was grown at pH 5.6 in solutions containing alkali metal chloride at concentrations ranging from 10{sup -5} to 10{sup -1} mol dm{sup -3}. When the radish were grown in culture solution with alkali metal ions of low concentrations (10{sup -5} and 10{sup -4} mol dm{sup -3}), Na, K, Rb and trace Li were detected in leaves, stems and roots while Cs was scarcely detected. However, the contents of Na, K, Li in these organs were the same as those in radish cultivated in pure water. An increase of Rb uptake was observed with an increased Rb ...

1995-05-01

69

Contribution of heavy metals and As-loaded lupin root mineralization to the availability of the pollutants in multi-contaminated soils  

International Nuclear Information System (INIS)

White lupin (Lupinus albus L.) is an annual crop that has been used for phytostabilization of acidified multi-contaminated soils. Once the culture cycle is over, after shoot harvesting, a progressive transference of contaminants from roots to soil may take place as decomposition of roots occurs. An incubation experiment with Cu, Zn, Cd, and As-loaded roots of white lupin and soils with different pH values and concentrations of these contaminants from the area affected by a mine spill at Aznalcollar (near Seville, Spain) was performed in order to assess the effect of the decomposition of the roots to the pH and (NH_4)_2SO_4-extractable levels of these pollutants in the soils. Pollutants loaded-roots were mineralized (56 d) at a ratio similar to animal manures (15.8-19.4% of total organic carbon) in soil. The estimated root inputs of contaminants in comparison to their extractable concentrations in soil were high, especially in the control, ...

2008-03-01

70

Safety performance indicators. Topical issues paper no. 5  

International Nuclear Information System (INIS)

Since its creation the nuclear industry has been struggling with the question of how safe is safe enough. Safety is a common goal to all involved in the design, operation and regulation of a nuclear installation. As a concept safety is not easy to define. However, there is a general understanding of what attributes a nuclear power plant should have in order to operate safely. The challenge lies in measuring the attributes. The new competitive open electricity market, in many countries throughout the world, is increasing the economic pressure on operators to lower operating costs without jeopardizing safety. Challenges are occurring at a rate that is unprecedented in the nuclear industry: competitiveness; downsizing; ageing; policy changes; reorganization; restructuring; mergers; globalization; and takeovers demand increasing attention to the management of safety. There are various means to measure safety performance, some of which are more qualitative in nature and others which through ...

2001-09-03

71

Extraction of molybdenum with a multielement liquid organic extraction system  

Science.gov (United States)

An extraction of molybdenum is described that provides a more predictable separation over a significantly wider acidity range than previously determined. The extraction of Mo appeared to be a function of HCl normality, with virtually complete separation above 3.6 N. A test was conducted to determine if the extraction was a function of just HCl concentration or of overall acidity. When the aqueous phases of the test sequence were all prepared at 1 N H/sub 2/SO/sub 4/ in addition to the HCl in each, the resulting extraction curves were essentially identical. Another test was performed to determine if Mo was being extracted as an iodide complex. Three aqueous solutions, which were 4 N in HCl and 26% (w/v) ascorbic acid, were prepared in culture tubes. When 25 ..mu..g of Mo was extracted from these solutions of 5 mL of organic phase, the KCl gave only a 16% extraction, the KBr gave only a 22% extraction, and the KI provided an essentially total ...

1985-10-01

72

Evaluation of influence of fertilizers in radium concentration from tomato culture; Avaliacao da influencia dos fertilizantes nos teores de radio encontrados na cultura do tomate  

Energy Technology Data Exchange (ETDEWEB)

The fertilizer contribution to radio uptake by tomato plants was assessed. Tomato and soil samples from four different plantations were analyzed: two using organic fertilizer and two using phosphate fertilizer. The Ra concentrations in eight of the most used tomato fertilizers were determined. The concentration values ranged from 14 to 221 Bq/kg of Ra-226 and from 25 to 176 Bq/kg of Ra-228. Owing to the low Ra concentration and to the used fertilizer quantities, the fertilizers would not increase significantly the Ra soil concentration. However, the highest soil to plant concentration ratios were found in the plantations using phosphate fertilizers. This outcome pointed out the higher Ra availability in the phosphate fertilizer plantations than in the organic fertilizer plantations, showing a possible positive influence of phosphate fertilizers for the tomato Ra uptake. Due to the phosphate fertilizer used, the dose could increase from 0,05 ...

2001-07-01

73

Effects of temperature and organic and inorganic nutrients on the growth of Chattonella marina (Raphidophyceae) from the Daya Bay, South China Sea  

British Library Electronic Table of Contents (United Kingdom)

The effects of temperature and different forms of nutrients on Chattonella marina growth have been investigated in strains isolated from the Daya Bay, the South China Sea. The strain of C. marina preferred high temperatures, with an optimal temperature of 25?C, and 18?C was the minimum for its survival. Higher cell number and growth rate were obtained in high nitrogen and phosphorus concentrations (500 ?g/L, 74 ?g/L) than under nutrient limitation. Nitrogen influenced the growth most, as the specific growth rate and maximum cell density were lower in nitrogenlimited cultures than noted under phosphorus limitation or under limitation from both. C. marina was capable of using many kinds of organic nitrogen sources including L-serine (L-Ser), glycine (Gly), alanine (Ala), L-threonine (L-Thr),...

2011-01-01

74

Resolving conflicting safety cultures  

International Nuclear Information System (INIS)

Several nuclear power plant sites have been wounded in the crossfire between two distinct corporate cultures. The traditional utility culture lies on one side and that of the nuclear navy on the other. The two corporate cultures lead to different perceptions of open-quotes safety culture.close quotes This clash of safety cultures obscures a very important point about nuclear plant operations: Safety depends on organizational learning. Organizational learning provides the foundation for a perception of safety culture that transcends the conflict between utility and nuclear navy cultures. Corporate culture may be defined as the knowledge, attitudes, and beliefs shared by employees of a given company. Safety culture is the part of corporate culture concerning shared attitudes and ...

1993-06-20

75

Nuclear safety culture star-class assessment system based BP neural network  

International Nuclear Information System (INIS)

In order to build the safety culture for nuclear power industry, it is important to evaluate the safety culture scientifically. Considering the traits of safety culture in the nuclear power industry, 24 safety culture assessment indexes are established from 4 aspects such as Safety consciousness, Safety attitude, Safety action and Safety actuality by using the SMART criteria. Safety culture star-class assessment criterion is presented and safety culture star-class assessment system is developed by using Visual Basic 6.0 and BP neural network. The system has a better generalization ability, and it can show exactly which phase the safety culture is in. Experimental results show that safety culture star-class assessment is practical and easy to perform. (authors)

2007-02-01

76

Effectively managing nuclear risk through human performance improvement  

International Nuclear Information System (INIS)

Full text: The US commercial nuclear industry has just completed an outstanding decade of plant performance. Safety levels and electric production are at unprecedented high levels and continue to exceed even high industry goals. Nuclear energy continues to keep the highest priority on performance improvement programs and highly trained/qualified people that maintain its record setting safety and reliability of operations. While the industry has maintained a consistently high level of performance, the advent of deregulation and the consolidation of NPP ownership, as well as the current climate of concern about both rising energy costs and availability of power, has raised the standard for nuclear energy's level of competitiveness in today's market place. The resulting challenge is how to more effectively manage risk and improve performance even further in a generally high performing organization. Newer technology and more training by themselves are not the answer. ...

77

Destruction of Salmonellae on poultry meat with lysozyme, EDTA, x ray, microwave, and chlorine  

International Nuclear Information System (INIS)

Lysozyme, ethylenediaminetetracetic acid, chlorine, x-irradiation and microwaves were used in experimental attempts to eliminate Salmonella senftenberg 775W or Salmonella typhimurium from turkey drumsticks and whole carcasses. Turkey drumsticks or whole carcasses were artificially contaminated with S. senfenberg 775W or S. typhimurium in concentrations ranging between 5 x 10"5 to 8 x 10"5 viable cells per ml. of contaminating fluid. After each treatment, samples were cultured, plated, and tested according to standard methods to determine the susceptibility of Salmonella organisms to the particular treatment. A 0.1 percent solution of lysozyme eliminated the S. senftenberg 775W at 22"0C within three hours. A 0.5 percent solution of ethylenediaminetetracetic acid failed to destroy the test organism under the same conditions. Eighty thousand rads of X-ray eliminated the test organism on turkey drumsticks ...

78

Regulation of Embryo Dormancy by Manipulation of Abscisic Acid in Kernels and Associated Cob Tissue of Zea mays L. Cultured in Vitro1  

UK PubMed Central (United Kingdom)

Sectors of Zea mays cobs, with and without kernels were cultured in vitro in the presence and absence of fluridone. Cultured kernels, cob tissue, and embryos developed...Full Text Available

1989-09-01

79

Mass Culture of Subanguina picridis and Its Bioherbicidal Efficacy on Acroptilon repens  

UK PubMed Central (United Kingdom)

A Russian knapweed (Acroptilon repens) shoot culture system, initiated from shoot tip culture, was used to generate a source of host plant tissue for the rearing of the nematode Subanguina...Full Text Available

1993-03-01

80

Effects of pH, Temperature, and Nutrients on Propionate Degradation by a Methanogenic Enrichment Culture  

UK PubMed Central (United Kingdom)

Enrichment cultures were used to determine the conditions promoting fastest methanogenic propionate degradation and growth by adapting the cultures to various physical and chemical conditions and measuring...Full Text Available

1987-07-01

81

Effects of Protons and HZE Particles on Glutamate Transport in Astrocytes, Neurons and Mixed Cultures  

Science.gov (United States)

... ions. MATERIALS AND METHODS Cell Culture Culture of NT2/D1 cells was carried out as described previously (18, 19). NT2 cells were plated at a density of 2. ... ...

82

Cultural Variation in Vigilance and Precaution Themes.  

Science.gov (United States)

Determine the degree to which different precautionary themes are dominant in various target populations and how these themes are acquired. Assess cultural signals of potential danger: are there different kinds of potential danger in different cultures. Do...

2009-01-01

83

Comprehensive Analysis of Blood Culture Performed at Nine University Hospitals in Korea  

UK PubMed Central (United Kingdom)

BackgroundOptimal blood culture performance is critical for successful diagnosis and treatment of sepsis. To understand the status of blood culture, we investigated several aspects...Full Text Available

2011-04-01

84

Comparison of Indoor Mercury Vapor in Common Areas of Residential Buildings with Outdoor Levels in a Community Where Mercury Is Used for Cultural Purposes  

UK PubMed Central (United Kingdom)

Elemental mercury has been imbued with magical properties for millennia, and various cultures use elemental mercury in a variety of superstitious and cultural practices, raising health concerns for...Full Text Available

2006-01-01

85

Integrating mission and strategy for nonprofit organizations  

CERN Document Server

Integrating mission and strategy for nonprofit organizations

2005-01-01

86

The Secretion of ?-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro  

UK PubMed Central (United Kingdom)

Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like...Full Text Available

1974-03-01

87

Sugarcane Culture and Syrup Production.  

Science.gov (United States)

The publication gives the basic information needed by growers of syrup sugarcane. It includes descriptions of the sugarcane plant characteristics needed in syrup varieties, the best varieties, cultural requirements and practices, and the diseases and inse...

1988-01-01

88

Culture of cells from beagles with bronchioloalveolar carcinoma  

International Nuclear Information System (INIS)

Cell cultures were prepared from lung tumors occurring in beagles following exposure to inhaled plutonium. Morphologic and growth characteristics of two of these cell lines are described.

1977-05-01

89

Culture and cooperation  

UK PubMed Central (United Kingdom)

Does the cultural background influence the success with which genetically unrelated individuals cooperate in social dilemma situations? In this paper, we provide an answer by analysing the data of Full Text Available

2010-09-12

90

CONSTRAINTS TO CRASSOSTREA ARIAKENSIS AQUACULTURE: SEASON AND METHOD OF CULTURE STRONGLY INFLUENCE SUCCESS OF GROW-OUT  

Science.gov (United States)

... of culture season and method on benefits of oyster aquaculture may be obtained without appreciable risk of unplanned ... ...

91

Distribution of polycyclic aromatic hydrocarbons in water and surface sediments from Daya Bay, China  

International Nuclear Information System (INIS)

Findings indicate an urgent need to establish a monitoring program for persistent organic pollutants in water and sediment. - Marine culture is thriving in China and represents a major component of the regional economy in coastal zones, yet the environmental quality of many of those areas has never been studied. This paper attempts to investigate the quality status of Daya Bay, a key aquaculture area in China. The levels of 16 polycyclic aromatic hydrocarbons (PAHs) were determined in water and sediment samples of the bay. The total concentrations of 16 PAHs varied from 4228 to 29325 ng l"-"1 in water, and from 115 to 1134 ng g"-"1 dry weight in sediments. In comparison to many other marine systems studied, the PAH levels in Daya Bay waters were relatively high, and at six sites they were sufficiently high (>10 #mu#g l"-"1) to cause acute toxicity. The PAH composition pattern in sediments suggest dominance by medium to high molecular weight ...

2003-02-01

92

Effect of plasma surface modification on the biocompatibility of UHMWPE  

Energy Technology Data Exchange (ETDEWEB)

In this paper active screen plasma nitriding (ASPN) is used to chemically modify the surface of UHMWPE. This is an unexplored and new area of research. ASPN allows the homogeneous treatment of any shape or surface at low temperature; therefore, it was thought that ASPN would be an effective technique to modify organic polymer surfaces. ASPN experiments were carried out at 120 {sup 0}C using a dc plasma nitriding unit with a 25% N{sub 2} and 75% H{sub 2} atmosphere at 2.5 mbar of pressure. UHMWPE samples treated for different time periods were characterized by nanoindentation, FTIR, XPS, interferometry and SEM. A 3T3 fibroblast cell line was used for in vitro cell culture experiments. Nanoindentation of UHMWPE showed that hardness and elastic modulus increased with ASPN treatment compared to the untreated material. FTIR spectra did not show significant differences between the untreated and treated samples; however, some changes were observed at ...

2010-10-01

93

[Legionella pneumonia caused by aspiration of hot spring water after sarin exposure].  

Science.gov (United States)

A 72-year-old man was exposed to the sarin gas attack in a Tokyo subway on March 20 th, 1995. After exposure, he noticed eye discomfort, chest tightness, headache and weakness of the lower limbs and oropharyngeal muscles. Despite these symptoms, he visited a hot spring on the same day with his family. On March 25 th, his muscle weakness progressed, and a low grade fever appeared. His muscle weakness disappeared 8 days after exposure to sarin, but respiratory failure rapidly developed, necessitating artificial ventilation within four day after hospitalization on March 28th. Chemotherapy with erythromycin, imipenem/cilastatin, and steroid pulse therapy was begu. PCR and culture of sputum collected by bronchofiberscopy were positive for Legionella pneumophila, serogroup I. His respiratory state improved, but subsequent infection with Pseudomonous aeruginosa. Enterobacter cloacae, and Candida tropicalis/glabrata caused his death 71 days after admission. Oropharyngeal ...

1998-03-01

94

Systematic Failure in a Stem Cell Fraud Case by a Poor Quality Culture  

International Nuclear Information System (INIS)

In the years 2004 and 2005, Korea became a prospective nation noteworthy in the world through the epochal development of genetic engineering in our country. Obstinate or incurable diseases were expected to be healed in a few years by a Korean geneticist. So many worldwide patients of obstinate or incurable diseases took notice of the research outcomes and organized an aid association to collect supporting funds. World renowned journals such as 'Nature' and 'Science' published Dr. Hwang's theses. A long and distant way remedy to obstinate diseases seemed to be closer at hand so early. However, a stem cell fraud case emerged throughout the world in late 2005, the pride of the Korean people was destroyed. With this as a momentum, voices of self reflection by scientific circles were raised. The purpose of this study is not in finding the truth of the stem cell research but to propose an effective management and control measures for the future in planning, implementing, ...

2006-05-25

95

Solar energy in buildings; L'energie solaire dans le batiment  

Energy Technology Data Exchange (ETDEWEB)

This document gathers the presentations given at the first French national meetings of solar energy for the development of solar systems in buildings. The meeting was organized over two days. The first day comprises 4 workshops about: urbanism and planning, cultural acceptability of solar energy in buildings (the OPAC 38 housing association, point of view on an energy information point, the Freiburg (Germany) solar region and marketing examples), technical integration to the structure (Clipsol solutions), and economical criteria (compared impacts of R and D public photovoltaic programs (USA, Japan, Germany, France, Italy), financing of rehabilitation projects, global approach of solar photovoltaic energy, technical solutions and strategy of products development, why and how to make an economical analysis of solar energy applications in the building industry). The second day comprises a plenary session and a round table: global status of solar ...

2003-07-01

96

Practice and experience of radiation protection and optimization (ALARA) management system in Daya Bay NPP during the past 10 years  

International Nuclear Information System (INIS)

With the practice of 10 years safe operation, Daya Bay Nuclear Power Station has established and continuously improved the management system for radiation protection and optimization (ALARA) which contains 3 basic requirements: all workers are trained, all employees are engaged in totally, and work management is implemented for the whole process. At the same time, strong efforts have been made to build the 'infrastructure' as a platform for its effective operation. This article introduces the contents and characteristics of the system and basic experiences of its effective implementation. In order to implement the management system effectively, it is necessary for NPPs to strengthen the responsibility system for radiation protection and the leading role of the radiation protection personnel, especially the role of technical support and supervision during the work with high radiation risk, emphasize the organic combination and actively mutual action with the safe ...

2004-05-01

97

Law as an organizational variable: an examination of the impact of law on the performance of the US Nuclear Regulatory Commission  

International Nuclear Information System (INIS)

The role of law in a federal regulatory agency is examined from an organizational perspective. While law is usually viewed in terms of its legal, political, and social value consequences, it is postulated that it also has significant organizational consequences. The impact of those consequences is examined in the case of a single agency, the US Nuclear Regulatory Commission. The legal process is shown to be a powerful organizational characteristic of the government administrative agency, beginning with statuatory definition of organization goals, structure, and procedures and ending with judicial review of actions. Agency lawyers are shown to represent a distinct professional subculture within this agency. Their values and orientations toward business, the role of regulation, and the role of nuclear utilities are different from the 95% of agency employees subculture based on a physical science/engineering background. It is concluded that agency effectiveness ...

98

Detecting exposure to environmental organic toxins in individual cells: towards development of a micro-fabricated device  

Energy Technology Data Exchange (ETDEWEB)

A new method is being developed to quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000-400 cm-1). Distinct changes have been observed in the IR spectra after treatment of human cells in culture medium with PAHs and OCs. The potential use of this method for detecting exposure to PAHs and OCs has been tested and compared to a reverse transcription polymerase chain reaction (RT-PCR) assay that quantifies increased expression of the CYP1A1 gene ...

1999-01-10

99

Application of spectroscopic techniques for the study of paper documents: A survey  

International Nuclear Information System (INIS)

For many centuries paper was the main material for recording cultural achievements all over the world. Paper is mostly made from cellulose with small amounts of organic and inorganic additives, which allow its identification and characterization and may also contribute to its degradation. Prior to 1850, paper was made entirely from rags, using hemp, flax and cotton fibres. After this period, due to the enormous increase in demand, wood pulp began to be commonly used as raw material, resulting in rapid degradation of paper. Spectroscopic techniques represent one of the most powerful tools to investigate the constituents of paper documents in order to establish its identification and its state of degradation. This review describes the application of selected spectroscopic techniques used for paper characterization and conservation. The spectroscopic techniques that have been used and will be reviewed include: Fourier-Transform Infrared ...

2009-06-01

100

Radiation effects on the shoot tip culture of chrysanthemum  

International Nuclear Information System (INIS)

Japanese (Mar 1974). Japan Mabuchi, Toshio Kuwada, Hikaru . Kagawa Univ.,

103

Organizational Culture and Changes of Command  

Science.gov (United States)

... The OER Support Form and NCOER Counselling Worksheet provide a way to match individual and organizational goals. ...

1991-04-26

111

An efficient in vitro plantlet regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through shoot tip culture  

British Library Electronic Table of Contents (United Kingdom)

An efficient micropropagation protocol was established for Cryptocoryne wendtii and Cryptocoryne becketti using shoot tips explants. Multiple shoots were induced from shoot tip explants of both species cultured on agar-gelled as well as liquid MS medium supplemented with 0.5?mg/L BA and 0.2?mg/L IBA (proliferation medium). The multiple shoots of both the species formed on agar-gelled as well as liquid medium were vigorously growing with well-developed roots and leaves after 4?weeks of culture. Highest number of multiple shoots was obtained from shoot tip explants of both the species cultured in liquid proliferation medium after 4?weeks of culture. The shoot tip explants of C. wendtii and C. becketti, that were cultured in liquid proliferation medium (2?weeks) followed by culturing on agar-...

2011-01-01

112

From gene manipulation to forest establishment: shoot cultures of woody plants can be a central tool  

Energy Technology Data Exchange (ETDEWEB)

Establishing germplasm of woody plants in microculture as shoot cultures has proved to be an effective method of overcoming many of the obstacles in working with these crops. Shoot cultures eliminate the changes associated with seasonal growth cycles and phase change and put large plants into a more manageable form. Well-established shoot cultures are central to successful clonal propagation systems for forest trees as well as to genetic improvement based on the use of cellular techniques such as protoplast manipulation. The physiological basis as to why tissues from shoot cultures are so readily manipulated is not well understood.

1985-05-01

113

Convoluted cells as a marker for maternal cell contamination in CVS cultures  

DEFF Research Database (Denmark)

In order to identify cells of maternal origin in CVS cultures, tissue from 1st trimester abortions were cultivated and the cultures stained in situ for X-chromatin. Convoluted cells and maternal fibroblasts were found to be positive. By chromosome analysis of cultures from 105 diagnostic placenta biopsies, obtained by the transabdominal route, metaphases of maternal origin were found in nine cases. In eight of these cases colonies of convoluted cells were observed. We conclude that convoluted cells are of maternal origin and are a reliable marker for maternal cell contamination in CVS cultures.

1987-01-01

114

Culture-area relation in Axelrod's model for culture dissemination  

CERN Document Server

A salient facet of Axelrod's model for culture dissemination or social influence is the existence of many multicultural absorbing states. The dependence of the number $C$ of different, coexisting cultures on the size $A$ of the territory or, equivalently, on the number of agents -- the culture-area relation -- is investigated through extensive simulations. This relation exhibits a strong qualitative dependence on the two parameters of the model, namely, the number $F$ of culture features and the number $q$ of values that each feature can take on. We find that a non-monotonous culture-area relation, for which the number of cultures decreases when the area grows beyond a certain size, occurs for $q$ smaller than a threshold value $q_c = q_c (F)$ provided that $F \\geq 3$. In the limit of infinite area, this threshold value signals the onset of a discontinuous ...

2009-01-01

115

A cultural research approach to instrument development: the case of breast and cervical cancer screening among Latino and Anglo women.  

Science.gov (United States)

To illustrate the implementation of a bottom-up approach to the study of culture in health disparities, this article describes the development of a cultural cancer screening scale (CCSS) using mixed methodologies. The aim was to identify cultural factors relevant to breast and cervical cancer screening, develop an instrument to assess them and examine its preliminary psychometric properties among Latin American (Latino) and non-Latino White (Anglo) women in Southern California. Seventy-eight Latino and Anglo women participated in semi-structured interviews, which were content coded based on Triandis' methods for the analysis of subjective culture. Based on the emerging cultural elements, items relevant to cancer screening were developed and pilot tested with 161 participants. After the instrument was refined, 314 Latino and Anglo women from various socioeconomic backgrounds ...

2010-09-23

116

X-ray absorption spectroscopy of bacterial sulfur globules  

Energy Technology Data Exchange (ETDEWEB)

Sulfur K-edge X-ray absorption spectroscopy is a powerful in situ probe of sulfur biochemistry in intact cells and tissues. Under favorable circumstances the technique can provide quantitative information on the chemical identify of the sulfur species that are present in a sample. Prange et al. have recently reported an X-ray absorption spectroscopic study of bacterial sulfur storage globules. Unfortunately there are substantial problems with the experimental technique employed that, they contend, lead to completely erroneous conclusions. In the more recent of their two papers Prange et al. employed a curve-fitting method similar to that used by us (for more than 10 years). In essence, the method employs simply fitting a linear combination of the spectra of standard compounds to that of the unknown, in this case cultures of bacterial cells. This type of analysis can provide quantitative estimates of the individual sulfur types in the sample, but is critically ...

2002-08-01

117

Is culture a determinant of financial development?  

British Library Electronic Table of Contents (United Kingdom)

This article investigates the missing link in the literature - whether informal institutions, or what is known as culture, can affect the level of financial development for a country? Our hypothesis stresses that the cultural dimensions of a country can have an impact on its financial set-up. We consider multiple dimensions of culture, identified in the literature by Tabellini (2008), to test our hypothesis. As culture evolves in the form of greater trust, control and other traits, individuals' attitudes towards financial market change, and they engage in greater financial transactions. This, in turn, leads to better financial development. Using quantile estimation technique for a cross section of 90 countries, we find that culture significantly influences the level of financial developmen...

2012-01-01

118

Cultural inheritance and diversification of diet in variable environments  

British Library Electronic Table of Contents (United Kingdom)

Both cultural inheritance and cultural diversification of diets may play an important role in animal evolution. Here we studied how diet innovation and cultural change relate to cultural inheritance in a changing environment. We did this by studying diet cultures in group foragers adapting to environmental change through learning, and the consequences this has for diet differentiation between groups. We used an individual-based model of `monkeys' that learn what to eat in a rich environment, and we changed resource species that are available in the environment. Relative to social influences on learning that arise spontaneously in groups, we found that more direct social learning, in the sense of observing another individual and copying what it eats, helps groups deal with high levels of en...

2009-01-01

119

The vomeronasal organ of the tammar wallaby  

UK PubMed Central (United Kingdom)

The vomeronasal organ is the primary olfactory organ that detects sexual pheromones in mammals. We investigated the anatomy of the vomeronasal organ of the tammar wallaby (Macropus eugenii),...Full Text Available

2008-08-01

120

Uptake and Intracellular Activity of Moxifloxacin in Human Neutrophils and Tissue-Cultured Epithelial Cells  

UK PubMed Central (United Kingdom)

The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At...Full Text Available

1999-01-01

121

Stimulation of albumin endocytosis by cationized ferritin in cultured aortic smooth muscle cells.  

UK PubMed Central (United Kingdom)

Anionic microdomains within the aortic smooth muscle cell (SMC) surface glycocalyx represent a potential barrier to the endocytosis of anionic plasma proteins. Cultured SMCs exposed briefly to cationized...Full Text Available

1985-12-01

122

Requirements for Extraction of Polyribosomes from Plant Callus Cultures 1  

UK PubMed Central (United Kingdom)

A procedure was developed to isolate polyribosomes from plant cell cultures. Tobacco callus extracted in 10 mm MgCl2, 80 mm KCl, 250 mm sucrose, and 140 mm...Full Text Available

1977-05-01

123

Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division.  

UK PubMed Central (United Kingdom)

Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol...Full Text Available

1990-02-01

124

Propagation of human embryonic and induced pluripotent stem cells in an indirect co-culture system  

UK PubMed Central (United Kingdom)

We have developed and validated a microporous poly(ethylene terephthalate) membrane-based indirect co-culture system for human pluripotent stem cell (hPSC) propagation, which allows real-time...Full Text Available

2010-03-05

125

Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations  

UK PubMed Central (United Kingdom)

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured...Full Text Available

2009-06-01

126

Political Culture and the Nature of Political Participation in Egypt.  

Science.gov (United States)

This thesis analyzes Egyptian political culture and the directive effect which it has upon political development, elite recruitment and the nature of political participation. The pervasive nature of Islam and the centrality of kinship remain the basic fac...

1980-01-01

127

Nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia.  

UK PubMed Central (United Kingdom)

Forty-eight mutants unable to reduce nitrate were isolated from "cowpea" Rhizobium sp. strain 32Hl and examined for nitrogenase activity in culture. All but two of the mutants had nitrogenase activity...Full Text Available

1977-02-01

128

Molecular and immunological comparison of human dihydropteridine reductase in liver, cultured fibroblasts and continuous lymphoid cells.  

UK PubMed Central (United Kingdom)

An antiserum was raised in a rabbit against highly purified human liver dihydropteridine reductase (EC 1.6.99.7). Dihydropteridine reductase from human liver, in human cultured fibroblasts and in continuous...Full Text Available

1981-07-01

129

Model for phase III autografts of epidermal cells cultured on a collagen-proteoglycan biomatrix.  

UK PubMed Central (United Kingdom)

The primary aim of this study was to develop a model system that uses epidermal cells (keratinocytes and accessory pigmented cells) cultured on a reconstituted basement membrane biomatrix for use in...Full Text Available

1989-10-01

130

Isolation of a bacterium resembling Pirellula species from primary tissue culture of the giant tiger prawn (Penaeus monodon).  

UK PubMed Central (United Kingdom)

During attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (Penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin...Full Text Available

1991-11-01

131

Hanford Cultural Resources Laboratory annual report for fiscal year 1990  

Energy Technology Data Exchange (ETDEWEB)

The Hanford Cultural Resources Laboratory (HCRL) was established by the US Department of Energy Field Office, Richland (RL) in 1987 as part of Pacific Northwest Laboratory. The HCRL provides support for managing the archaeological, historical, and cultural resources of the Hanford Site, Washington, in a manner consistent with federal statutes and regulations. This report summarizes activities of the HCRL during fiscal year (FY) 1990. The HCRL responsibilities have been set forth in the Hanford Cultural Resources Management Plan (HCRMP) as a prioritized list of tasks. The task list guided cultural resources management activities during FY 1990 and is the outline for this report. In order, these tasks were to (1) conduct cultural resource reviews, (2) develop an archaeological resources protection plan, (3) monitor the condition of known archaeological sites, (4) plan a curation ...

1991-11-01

132

Growth temperature reversibly modulates the virulence of Legionella pneumophila.  

UK PubMed Central (United Kingdom)

In chemostat culture, the virulence of two strains of Legionella pneumophila was shown to be significantly (P < 0.05) reduced when the culture temperature was lowered from 37 to 24 degrees C....Full Text Available

1994-07-01

133

Effects of Cycling Temperatures on Fiber Metabolism in Cultured Cotton Ovules 1  

UK PubMed Central (United Kingdom)

The effects of temperature on rates of cellulose synthesis, respiration, and long-term glucose uptake were investigated using cultured cotton ovules (Gossypium hirsutum L. cv Acala...Full Text Available

1992-10-01

134

Discovering Networks of Perturbed Biological Processes in Hepatocyte Cultures  

UK PubMed Central (United Kingdom)

The liver plays a vital role in glucose homeostasis, the synthesis of bile acids and the detoxification of foreign substances. Liver culture systems are widely used to test adverse effects of drugs...Full Text Available

135

Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp  

UK PubMed Central (United Kingdom)

A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility...Full Text Available

1990-11-01

136

Cultural beliefs that may discourage breastfeeding among Lebanese women: a qualitative analysis  

UK PubMed Central (United Kingdom)

BackgroundAlthough the health benefits of breastfeeding are well established, early introduction of formula remains a common practice. Cultural beliefs and practices can have an...Full Text Available

137

Cultural and Contextual Influences on Parenting in Mexican American Families  

UK PubMed Central (United Kingdom)

Family stress theory can explain associations between contextual stressors and parenting. However, the theory has not been tested among Mexican Americans or expanded to include cultural-contextual...Full Text Available

2009-02-01

138

Cell-density-dependent Changes in the Metabolism of Chloronema Cell Cultures  

UK PubMed Central (United Kingdom)

In the growing chloronema cell suspension cultures of the moss Funaria hygrometrica Hedw., activities of several enzymes have been found to be cell-density-dependent. Cyclic nucleotide...Full Text Available

1979-07-01

139

Implementation of an inclusive radiation monitoring system in the Bragin district in Belarus  

Energy Technology Data Exchange (ETDEWEB)

This paper presents results from one of the projects developed within the general framework of the international Programme 'CORE' (Cooperation for Rehabilitation in Belarus). The overall objective of the programme is to make sustainable improvements to the living conditions of the inhabitants of the territories affected by the Chernobyl disaster. The CORE Programme is currently developed in four contaminated districts of Belarus (Bragin, Cherchersk, Slavgorod and Stolyn) in the following four areas: economic and social development; health care and surveillance; education and culture; and radiological quality. The project that is presented here refers specifically to the field of 'radiological quality' and will last until the end of 2008. The project named 'implementation of an inclusive radiation monitoring system' was launched in April 2004 in the Bragin district, which is one of the most contaminated areas in ...

2006-07-01

143

Methods to improve the fertility of poinsettia  

British Library Electronic Table of Contents (United Kingdom)

`Success' or `Red Splendor' plants propagated by shoot tip culture or embryogenesis had higher pollen germination than those propagated by cutting. All clones of `Pepride', `Peter Star' and `Nobel Star' propagated by cutting or by embryogenesis or shoot tip culture did not have viable pollen. `Peter Star' mutants through mutagenesis could recover pollen viability. When both parents were propagated by tissue culture, fruit set increased compared to when one or both parents were propagated by cuttings. Furthermore, the number of the harvested seeds doubled per pollination set, when the tissue culture parents were used instead of cutting-propagation plants.

2008-01-01

144

Educational research in Confucian cultural contexts: reflections on methodology  

British Library Electronic Table of Contents (United Kingdom)

This paper engages in a critical discussion of the key features delineated by the other authors in this Special Issue thus serving as an end-piece for the issue. In particular it touches on three significant concepts. Firstly, it reinforces the importance of context, particularly cultural context in the conduct of research in Confucian cultural settings. Second, as an illustration, it offers a critical perspective on the cultural assumptions that underpin the development of Chinese higher education. Finally, it concludes with some reflections on how other papers in this Special Issue contribute to our understanding of research in CHC contexts.

2011-01-01

145

Beyond the Flatline  

British Library Electronic Table of Contents (United Kingdom)

Abstract High Post-Modernism with its understanding of the significance of the image anticipated the flattening of culture, first in advertising and the printed media, and more recently online. Sam Jacob of FAT speculates on architecture's relationship with the Internet, -a flatland of undifferentiated information-, and how Radical Post-Modernism might be best placed to resist the ubiquity of global culture while reanimating the social and cultural agenda of Modernism and Post-Modernism. Copyright 2011 John Wiley & Sons, Ltd.

2011-01-01

146

Radiation Protection of the Organism (Selected Chapters)  

Science.gov (United States)

... biologically important compounds is sharply lowered with passage of the solutions to the cell an organ, where their sensitivity ...

1970-12-11

147

Mad Cow: Why Organic Beef is Safer than Conventional Beef in theU.S.  

Wastenet

... In order to produce organic livestock feed, feed mills must be inspected and certified. If they produce both organic and non-organic feed, they must implement procedures, backed up by records, to prevent the commingling of organic and non-organic feed. This includes steps to clean storage bins and mixing and bagging equipment prior to producing batches of organic feed. Organic feed mills also must prevent ...

150

Thermokinetic investigation of effects of carbon source on petroleum bacterial growth  

Energy Technology Data Exchange (ETDEWEB)

The growth power-time curves of a strain of petroleum bacteria, B-2, in various kinds of cultures containing different kinds of carbon sources, glucose, n-tetradecane, n-hexadecane and n-octadecane, and different kinds of microemulsions have been determined by using a 2277 Thermal Activity Monitor. The curves showed a single peak for cultures containing a single carbon source, glucose, and two peaks for cultures containing two kinds of carbon sources, glucose and one of the n-alkanes. The first peak indicated that bacteria grew by consuming glucose and the second peak indicated that bacteria grew by consuming n-alkane. The curves were complex when the bacterium grows in a microemulsion culture. According to a kinetic equation of bacterial growth under limited conditions, the rate constants of bacterial growth were obtained. The results showed that the microemulsion culture was more ...

2002-02-07

151

P2Y receptors on astrocytes and microglia mediate opposite effects in astroglial proliferation  

British Library Electronic Table of Contents (United Kingdom)

Nucleotides released upon brain injury signal to astrocytes and microglia playing an important role in astrogliosis, but the participation of microglia in the purinergic modulation of astrogliosis is still unclear. Highly enriched astroglial cultures and co-cultures of astrocytes and microglia were used to investigate the influence of microglia in the modulation of astroglial proliferation mediated by nucleotides. In highly enriched astroglial cultures, adenosine-5?-triphosphate (ATP), adenosine 5?-O-(3-thio)-triphosphate (ATP?S), adenosine 5?-O-(3-thio)-diphosphate (ADP?S; 0.01?1?mM), and adenosine-5?-diphosphate (ADP; 0.1?1?mM) increased proliferation up to 382%, an effect abolished in co-cultures containing 8% of microglia. The loss of ATP proliferative effect in co-cultures is supporte...

2011-01-01

152

Malignancy without immortality? Cellular immortalization as a possible late event in melanoma progression  

British Library Electronic Table of Contents (United Kingdom)

Summary Cell senescence is a permanent growth arrest following extended proliferation. Cultured cancer cells including metastatic melanoma cells often appear immortal (proliferate indefinitely), while uncultured benign nevi (moles) show senescence markers. Here, with new explantation methods, we investigated which classes of primary pigmented lesions are typically immortal. Nevi yielded a few proliferating cells, consistent with most nevus cells being senescent. No nevus culture (0/28) appeared immortal. Some thin and thick melanoma cultures proved immortal under these conditions, but surprisingly few (4/37). All arrested cultures displayed three senescence markers in some cells: -galactosidase, nuclear p16, and heterochromatic foci/aggregates. However, melanoma cultures also showed featur...

2011-01-01

153

Importance of serum source for the in vitro replicative senescence of human bone marrow derived mesenchymal stem cells  

British Library Electronic Table of Contents (United Kingdom)

Abstract Human mesenchymal stem cells (hMSCs) may be used for therapeutic applications. Culture conditions such as the serum source may impact on cell quality and the onset of replicative senescence. We have examined the effect of culturing hMSCs in autologous serum (AS) versus fetal bovine serum (FBS) on factors involved in in vitro replicative senescence. hMSCs from four donors were cultured in 10% FBS or 10% AS until they reached senescence. Cells were harvested at early passage and near senescence to study factors known to be involved in cellular senescence. The number of population doublings till senescence was similar for cells cultured in FBS, but varied greatly for hMSCs cultured in AS. FBS cells accumulated in S phase of cell cycle. This could not be explained by increased express...

2011-01-01

154

Heightened awareness of a researcher's own culture through carrying out research on development cooperation  

British Library Electronic Table of Contents (United Kingdom)

In this paper I argue that when a researcher is a research instrument, it is likely that the researcher would develop a heightened awareness of his/her own cultural conditioning, especially in the case of cross-cultural studies. And that such awareness would make him/her realise that one's own cultural background may indeed have an influence on formulating his/her research methodology. The paper is based on my own research project on development cooperation between Japan and Cambodia, whereby I examined the perceptions of Cambodians and Japanese on what an appropriate power relationship between donors and recipients should be. At the outset of my research project, I paid little attention to my own cultural condition and the role it might play in my study, though I was aware that being Japa...

2011-01-01

155

Chondrocytes and bone marrow-derived mesenchymal stem cells undergoing chondrogenesis in agarose hydrogels of solid and channelled architectures respond differentially to dynamic culture conditions  

British Library Electronic Table of Contents (United Kingdom)

Abstract The objective of this study was to investigate how a combination of different scaffold architectures and rotational culture would influence the functional properties of thick cartilaginous tissues engineered using either chondrocytes or bone marrow-derived mesenchymal stem cells (BM-MSCs). Expanded porcine chondrocytes and BM-MSCs were suspended in 2% agarose and cast in custom-designed moulds to produce either regular solid or channelled construct cylinders. The study consisted of three seperate experimental arms. First, chondrocyte and BM-MSC constructs were cultured in free swelling conditions for 9 weeks. Second, constructs were subjected to rotational culture for a period of 3 weeks. Finally, BM-MSC-seeded constructs were subjected to delayed rotational culture, in which cons...

2011-01-01

156

Second international symposium on nuclear power plant life management. Book of extended synopses  

International Nuclear Information System (INIS)

The world's fleet of nuclear power plants is, on average, more than 20 years old. Even though the design life of a nuclear power plant is typically 30-40 years, it is quite feasible that many plants will be able to operate in excess of their design lives, provided that nuclear power plant engineers demonstrate by analysis, trending, equipment and system upgrades, increased vigilance, testing and ageing management that the plant will operate safely. In the operation of nuclear power plants, safety should be always the prime consideration. Plant operators and regulators must always ensure that plant safety is maintained, and where possible enhanced, during a plant's operating lifetime. Nuclear power plant life management (PLiM) has gained increased attention over the past decade, and effective ageing management of systems, structures and components (SSCs) is a key element in PLiM for the safe and reliable long term operation of nuclear power plants. A PLiM programme is an effective tool ...

2007-10-15

157

Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of Calophyllum inophyllum L.  

British Library Electronic Table of Contents (United Kingdom)

Cell suspension cultures were initiated separately from leaf and nodal/internodal calluses for the study of influence of hormones and medium components on biomass growth and expression of dipyranocoumarins. Highest 6.2 times biomass was enhanced in suspension cultures of nodal/internodal callus supplemented with threefold total sulphate. Picloram 8.28mM along with BAP 8.88mM enhanced 295.05 times inophyllum A in suspension cultures of leaf callus whereas IBA 14.70mM along with BAP 4.44mM in suspension cultures of leaf callus enhanced 1065 times inophyllum B. IBA 4.90mM alone in suspension cultures of nodal/internodal callus enhanced maximum 616 times inophyllum C. Only IBA 9.80mM in suspension cultures of leaf callus enhanced 23.22 times inophyllum P. Variation in nitrate and sulphate had ...

2009-01-01

158

Education for All by 2015: Will We Make It?  

Science.gov (United States)

Seven years ago 164 governments, together with partner organizations from around the world, made a collective commitment to dramatically expand educational opportunities for children, youth, and adults by 2015. Participants at the World Education Forum in Dakar, Senegal, endorsed a comprehensive vision of education, anchored in human rights, affirming the importance of learning at all ages and emphasizing the need for special measures to reach the poorest, most vulnerable and most disadvantaged groups in society. This sixth edition of the "Education for All (EFA) Global Monitoring Report" assesses the extent to which these commitments are being met. There is clearly a "Dakar effect," evidence that rallying around common goals can mobilize countries to empower individual lives. Partly because of the abolition of tuition fees, more children are enrolled in school than in 2000, with the sharpest increases in the regions farthest from the goals set in Dakar. Many ...

2006-12-01

159

Toxicity of N-substituted aromatics to acetoclastic methanogenic activity in granular sludge  

Energy Technology Data Exchange (ETDEWEB)

N-substituted aromatics are important priority pollutants entering the environment primarily through anthropogenic activities associated associated with the industrial production of dyes, explosives, pestides, and pharmaceuticals. Anaerobic treatment of wastewaters discharged by these industries could potentially be problematical as a result of the high toxicity of N-substituted aromatics. The objective of this study was to examine the structure-toxicity relationship of N-substituted aromatic compounds to acetoclastic methanogenic bacteria. The toxicity was assayed to serum flasks by measuring methane production in granular sludge. Unacclimated cultures were used to minimize the biotransformation of the toxic organic chemicals during the test. The nature and the degree of the aromatic substitution were observed to have a profound effect on the toxicity of the test compound. Nitroaromatic compounds were, on the average, over 500-fold more toxic ...

1995-11-01

160

Memento of decision makers: the national organizations involved in the mastery of greenhouse gas emissions; Memento des decideurs: les collectivites territoriales engagees dans la maitrise des emissions de gaz a effet de serre  

Energy Technology Data Exchange (ETDEWEB)

In front of the risks linked with the increase of the greenhouse gas emissions in the atmosphere, the decision makers must take into consideration first, the scientific advice of climatic change experts, and second, the considerable inertia of the climatic system. Thus, any action implemented so far will have an impact all along the 21. century and later whatever the future human activities. The aim of this memento is to sensibilize the decision makers about the possible consequences of their choice in terms of volume of greenhouse gases and of medium- and long-term evolution: 1 - stakes, role of local decision-makers (greenhouse effect and climatic change, France's international commitment, stakes, liabilities of local decision makers, decentralization laws, local plans of fight against greenhouse effect, public information and dialogue); 2 - urbanism and transports (urban displacements, alternatives to individual cars, collective transportation systems, parking, inter-region ...

2003-07-01

161

Genome analysis of F. nucleatum sub spp vincentii and its comparison with the genome of F. nucleatum ATCC 25586.  

Science.gov (United States)

We present the draft genome sequence and its analysis for Fusobacterium nucleatum sub spp. vincentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN). A total of 441 FNV open reading frames (ORFs) with no orthologs in FN have been identified. Of these, 118 ORFs have no known function and are unique to FNV, whereas 323 ORFs have functional orthologs in other organisms. In addition to the excretion of butyrate, H2S and ammonia-like FN, FNV has the additional capability to excrete lactate and aminobutyrate. Unlike FN, FNV is likely to incorporate galactopyranose, galacturonate, and sialic acid into its O-antigen. It appears to transport ferrous iron by an anaerobic ferrous transporter. Genes for eukaryotic type serine/threonine kinase and phosphatase, transpeptidase E-transglycosylase Pbp1A are found in FNV but not in FN. Unique ABC transporters, cryptic phages, and three types of restriction-modification systems have been identified in FNV. ORFs for ...

2003-06-01

162

Evaluation of fungal bio burden and mycotoxins presence in irradiated samples of medicinal plants purchased from wholesale and retail market  

International Nuclear Information System (INIS)

This present study evaluated the effect of gamma radiation on the fungal survival in packed medicinal plants, purchased from wholesale and retail market, in different period (0 and 30 days) after the treatment. Five kind of medicinal plants (Peumus boldus, Camellia sinensis, Maytenus ilicifolia, Paullinia cupana and Cassia angustifolia), were collected from different cities of Sao Paulo State, and submitted to irradiation treatment using a 60Co source (type Gammacell 220) with doses of 5,0 kGy and 10 kGy and at dose rate of 3.0 kGy/h. Non-irradiated samples (control group) were used for fungal counts and serial dilutions from 10-1 to 10-6 of the samples were seeded in duplicates and plated using the surface culture method in Dichloran 18% Glycerol Agar (DG 18) and were counted after five days at 25 deg C. The control group revealed the presence of genera Aspergillus and Penicillium, which are known as toxigenic fungi and a few samples of control group were within ...

2006-12-20

163

Does tea tree oil have a place in the topical treatment of burns?  

Science.gov (United States)

Burnaid is a sorbalene-based cream containing 40 mg/g of tea tree oil and 1 mg/g of triclosan. This investigation was carried out to determine the effect of Burnaid, a commercial tea tree oil preparation, against Enterococcus faecalis (ATCC29212), Staphylococcus aureus (ATCC29213), Escherichia coli (ATCC25922), and Pseudomonas aeruginosa (ATCC27853), with the activity of the base product in the commercial preparation. The organisms were suspended in sterile saline (0.5 McFarland Standard) and inoculated onto horse blood agar (E. faecalis and S. aureus) or Mueller-Hinton agar (E. coli and P. aeruginosa). One hundred microliters of Burnaid unsterilized, Burnaid sterilized and the base product (Tinasolve) were placed in duplicate in wells cut into the agar plates. Sterility and inactivation cultures were also performed on the samples. None of the samples were found to be contaminated with bacteria prior to testing. Only S. aureus and E. coli ...

1997-06-01

164

Biphasic calcium sulfate dihydrate/iron-modified alpha-tricalcium phosphate bone cement for spinal applications: in vitro study  

Energy Technology Data Exchange (ETDEWEB)

In this study, the cytocompatibility of new 'iron-modified/alpha-tricalcium phosphate (IM/alpha-TCP) and calcium sulfate dihydrate (CSD)' bone cement (IM/alpha-TCP/CSD-BC) intended for spinal applications has been approached. The objective was to investigate by direct-contact osteoblast-like cell cultures (from 1 to 14 days) the in vitro cell adhesion, proliferation, morphology and cytoskeleton organization of MG-63 cells seeded onto the new cements. The results were as follows: (a) quantitative MTT-assay and scanning electron microscopy (SEM) showed that cell adhesion, proliferation and viability were not affected with time by the presence of iron in the cements; (b) double immunofluorescent labeling of F-actin and alpha-tubulin showed a dynamic interaction between the cell and its porous substrates sustaining the locomotion phenomenon on the cements' surface, which favored the colonization, and confirming the ...

2010-04-15

165

Optical properties of proton-irradiated polymers  

Energy Technology Data Exchange (ETDEWEB)

Recently, organic semiconducting materials have gained a broad interest due to their potential for organic electronic devices such as organic light emitting diode (OLED), organic photovoltaic devices and organic field-effect transistors (OFETs). Optical properties of organic semiconducting materials are important for practical application. For example, the power conversion efficiency of organic photovoltaic devices is mainly affected by absorption properties of organic materials. Proton irradiation is one of the efficient methods to change the optical properties of organic materials. In this paper, we investigate the changes of optical properties of various polymers using the proton irradiation.

2009-05-15

166

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells in irradiated bone marrow cell culture in vitro  

Energy Technology Data Exchange (ETDEWEB)

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells after irradiation were studied in the long-term culture of mouse bone marrow cells in vitro. No difference was observed in the survival of the stem cells among cultures in which 0 - 10/sup 7/ cells were re-inoculated on the adherent cell colonies in the culture flask. Stem cells showed a significant proliferation within 1 week and the number of the stem cells exceeded the control in 3 weeks after irradiation in the cultures with less than 10/sup 6/ re-inoculated cells per flask. In contrast, there was a considerable delay in the onset of stem cell proliferation after irradiation in the culture with 10/sup 7/ cells per flask. Based on these results, a possibility that a stimulator of stem cell proliferation, released from irradiated stromal cells, is cancelled by an inhibitory factor produced ...

1981-12-01

167

Context based inferences in research methodology: the role of culture in justifying knowledge claims  

British Library Electronic Table of Contents (United Kingdom)

Drawing on work in epistemology and the philosophy of science, this paper seeks to provide very general reasons for why a comparative perspective needs to be applied to the inferential procedures of research methodologies where these concern the issue of justifying knowledge claims. In particular, the paper explores the role of culture on a number of important patterns of reasoning that figure in inferential arguments in research methodologies. The patterns examined are induction, both enumerative and analytical, hypothetico-deductive reasoning, and abductive inference. In each case it is argued that substantive theories about the world, including cultures, significantly affect inferential procedures. Examples chosen to illustrate this in more detail mostly reflect the impact of Confucian ...

2011-01-01

168

Cell cultures are more sensitive than Saccharamoyces cervisiae tests for assessing the toxicity of aquatic pollutants  

Energy Technology Data Exchange (ETDEWEB)

Cultured fish and human cells have been used as bioassay systems for the evaluation of the toxicity of aquatic pollutants. Numerous assays using bacteria and yeast have also been used for such purposes. The authors report the toxicity of aquatic pollutants (Cd, Hg, and Ni), using cell culture systems and the yeast Saccharomyces cervisiae test. Cd, Hg, and Ni were chosen as model compounds of pollutants because the related toxicity is now fairly well established.

1988-07-01

169

Short-Term Metal/Organic Interface Stability Investigations of Organic Photovoltaic Devices: Preprint  

Energy Technology Data Exchange (ETDEWEB)

This paper addresses one source of degradation in OPV devices: the metal/organic interface. The basic approach was to study the completed device stability vs. the stability of the organic film itself as shown in subsequent devices fabricated from the films.

2008-05-01

170

Policy Tool Box.indd  

Wastenet

Labeling of organic res- taurants, cantinas, etc.

172

To talk or not to talk: exploring culturally diverse patients' health information communication choices.  

Science.gov (United States)

As care shifts from institutional to home- and community-based settings, consumer health information technology (IT) must be designed to support patients' new health information management responsibilities. We developed and piloted a new methodology grounded in social network analysis and human factors engineering to explore two often overlooked aspects of this phenomenon: the task of health information communication with members of the social network and the context of culture. Such knowledge is necessary to inform the appropriate design of consumer health IT. We asked a culturally diverse sample of participants to describe what, to whom, why, and how they communicate health information and to provide direct feedback about the methodology. The methodology was acceptable to all participants and able to capture similarities and differences in their health information communication practices. Prior to the main study we will need to refine the ...

2010-11-13

173

The actin content of fibroblasts.  

UK PubMed Central (United Kingdom)

Cultures of chick skin fibroblasts were dissolved in solutions of sodium dodecyl sulphate, and their entire protein content was examined by gel electrophoresis. The most abundant species migrated in...Full Text Available

1975-05-01

174

The Structure of Plant Cell Walls  

UK PubMed Central (United Kingdom)

The molecular structure, chemical properties, and biological function of the xyloglucan polysaccharide isolated from cell walls of suspension-cultured sycamore (Acer pseudoplatanus)...Full Text Available

1973-01-01

175

The I3I Model; Identifying Cultural Determinants of Information ...  

Science.gov (United States)

... Information behavior in dynamic group work contexts: Interwoven situational awareness, dense social networks and contested collaboration in ...

2009-06-01

176

Production of Amylase in Liquid Culture by a Strain of Aspergillus oryzae  

UK PubMed Central (United Kingdom)

The effect of different media and pH on the formation of amylase by Aspergillus oryzae EI 212 is described. Depending upon the composition of the medium and growth...Full Text Available

1970-04-01

177

Novel Cytotoxic Vectors Based on Adeno-Associated Virus  

Wastenet

positive primary PymT breast cancer cells in primary co-cultured tumor tissue, suggesting target specificity of

178

Nineteen Cases of Plague in Arizona  

UK PubMed Central (United Kingdom)

We review the cases of 19 successfully treated plague patients, with emphasis on the clinical and epidemiologic features of the disease. Proper staining and culturing of bubo aspirates; prompt institution...Full Text Available

1985-05-01

179

Metamorphosis of Confucian Heritage Culture and the possibility of an Asian education research methodology  

British Library Electronic Table of Contents (United Kingdom)

This paper opens with a critical analysis of a paradox in contemporary educational research in and about Confucian Heritage Culture (CHC): the assumption that national boundaries coincide with those of a distinct and homogeneous culture, which consistently renders a rather homogenous set of educational phenomena, and collides against a more widely accepted discourse - culture transcends geographical frontiers and is ever evolving in character. It is claimed that this paradox is due to the fact that a thin conception of CHC competes neck-and-neck with a thick conception of it. This paper also addresses the possibility of an ad hoc education research methodology in and about CHC and its compliance issues regarding the mainstream Western research dynamics and philosophy of science. Confucian ...

2011-01-01

180

Invasive Species: State Resources - Colorado  

Science.gov (United States)

of Concern; Parks; Management; Monitoring Exotic and Invasive Species Northern Arizona University. Canyons, Cultures, and Environmental Change. Species of Concern; Contacts;...

2011-10-01

181

Hispanic Cultural Influences on Medical Practice  

UK PubMed Central (United Kingdom)

Because the proportion of Hispanic patients is increasing rapidly, most physicians are now coming into daily contact with Hispanics. In addition to obvious difficulty with oral communication, Hispanics...Full Text Available

1983-10-01

182

Filopodia  

UK PubMed Central (United Kingdom)

The processes of neuronal outgrowth and guidance have typically been studied in classic 2D cell culture systems that do not recapitulate topographical cues present in the in vivo extracellular matrix...Full Text Available

2011-07-01

183

Factors Influencing the Effectiveness of Swimming Pool Bactericides  

UK PubMed Central (United Kingdom)

Techniques for culturing, harvesting, and testing bacteria to evaluate bactericidal chemicals for swimming pools are described. Concentrations of 25, 50, and 100 mg of the chlorine stabilizer cyanuric...Full Text Available

1967-05-01

184

Experience of isolated sleep paralysis in clinical practice in Nigeria.  

UK PubMed Central (United Kingdom)

The supernatural fears associated with the experience of isolated sleep paralysis in the culture of developing countries is sometimes associated with the evolution of somatic symptoms of psychological...Full Text Available

1992-06-01

185

Evaluation of the microbiology of chronic ethmoid sinusitis.  

UK PubMed Central (United Kingdom)

In a prospective study, patients with the diagnosis of chronic ethmoid sinusitis were evaluated microbiologically by using biopsy specimens of the ethmoid sinus mucosa. Microbiology cultures were performed...Full Text Available

1991-11-01

186

Eukaryotic Diversity in an Anaerobic Aquifer Polluted with Landfill Leachate?  

UK PubMed Central (United Kingdom)

Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and...Full Text Available

2008-07-01

187

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform  

British Library Electronic Table of Contents (United Kingdom)

Abstract The glycosylation profile of therapeutic antibodies is routinely analyzed throughout development to monitor the impact of process parameters and to ensure consistency, efficacy, and safety for clinical and commercial batches of therapeutic products. In this study, unusually high levels of the mannose-5 (Man5) glycoform were observed during the early development of a therapeutic antibody produced from a Chinese hamster ovary (CHO) cell line, model cell line A. Follow up studies indicated that the antibody Man5 level was increased throughout the course of cell culture production as a result of increasing cell culture medium osmolality levels and extending culture duration. With model cell line A, Man5 glycosylation increased more than twofold from 12% to 28% in the fed-batch process...

2011-01-01

189

Development and Reproduction of grape phylloxera on irradiated in vitro cultured rootstocks  

International Nuclear Information System (INIS)

The ability of a local strain of grape phylloxera to develop and reproduce on irradiated in vitro cultured rootstocks (Ru140, R99 and 3309C and one local variety 'Helwani' was determined. The results showed that there was great variation in developmental time and reproduction of phylloxera between irradiated and unirradiated in vitro cultured plants. Survival, number of eggs and mean developmental time were greatly reduced when phylloxera was reared on irradiated rootstocks. Based on the examined biological parameters of phylloxera, all tested rootstocks would be more resistant toward such destructive insect when they were irradiated. Thus, phylloxera resistance was enhanced when in vitro cultured plants were treated with low doses of gamma irradiation. (author)

190

Detection and Identification of Bacteria by Gas Chromatography1  

UK PubMed Central (United Kingdom)

Ether extracts of cultures of 29 strains representing 6 species of Bacillus, and of individual strains of Escherichia coli, Aerobacter aerogenes, and Pseudomonas...Full Text Available

1966-07-01

191

Cultures of Death and Politics of Corpse Supply  

UK PubMed Central (United Kingdom)

SummaryNineteenth-century Vienna is well known to medical historians as a leading centre of medical research and education, offering easy access to patients and corpses to students...Full Text Available

2008-01-01

192

Context, Culture, and Connection: Avoiding the Counter ...  

Science.gov (United States)

... Page 25. US Department of Defense, Joint Operation Planning and Execution System, Vol 1, Planning Policies and Procedures (CJCSM 3122.01A). ...

2008-04-23

193

Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces  

UK PubMed Central (United Kingdom)

BackgroundOsseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility...Full Text Available

194

Apoptosis at Inflection Point in Liquid Culture of Budding Yeasts  

UK PubMed Central (United Kingdom)

Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known ...Full Text Available

195

Accumulation, Activity and Localization of Cell Cycle Regulatory Proteins and the Chloroplast Division Protein FtsZ in the Alga Scenedesmus quadricauda under Inhibition of Nuclear DNA Replication  

British Library Electronic Table of Contents (United Kingdom)

Synchronized cultures of the green alga Scenedesmus quadricauda were grown in the absence (untreated cultures) or in the presence (FdUrd-treated cultures) of 5-fluorodeoxyuridine, the specific inhibitor of nuclear DNA replication. The attainment of commitment points, at which the cells become committed to nuclear DNA replication, mitosis and cellular division, and the course of committed processes themselves were determined for cell cycle characterization. FdUrd-treated cultures showed nearly unaffected growth and attainment of the commitment points, while DNA replication(s), nuclear division(s) and protoplast fission(s) were blocked. Interestingly, the FdUrd-treated cells possessed a very high mitotic histone H1 kinase activity in the absence of any nuclear division(s). Compared with the ...

2008-01-01

196

A Preliminary Study on Dressing Patterns and Incidence of Candidiasis  

UK PubMed Central (United Kingdom)

The incidence of candidiasis in two groups of voluntary participants wearing tight and loose fitted dresses was investigated by both microscopic and cultural techniques for a period of two months....Full Text Available

1982-02-01

197

A Cultural Resources Survey Testing, and Geomorphic ...  

Science.gov (United States)

... Aerial photographs were provided by Mr. Grover P. ... Dr. Beverely J. Watkins conducted the records search and wrote the historic research. x,/ ...

1987-04-01

199

Role of organic acids in promoting colloidal transport of mercury from mine tailings  

CERN Document Server

Role of organic acids in promoting colloidal transport of mercury from mine tailings

2005-01-01

200

--No Title--  

Science.gov (United States)

Nitrogens, Sulfurs, Isotopes, and Hydrocarbons Gases Elements Ions and Inorganic Acids Organic Compounds Co-eluting Organics Hopanes, Cholestanes, and Sterols Pesticides,...

2011-08-19

201

Tissue culture process for the clonal production of loblolly pine plantlets  

Energy Technology Data Exchange (ETDEWEB)

A tissue culture process, organogenesis, is described which involves the stepwise production of a shoot apex, which grows to have leaves and stem and finally roots, culminating in the production of a complete plant. The production and subsequent development of numerous adventitious shoots, preferably from adult tree tissues offers the greatest potential for commercial cloned plantlet production. The process describes these steps for Pinus taeda, the loblolly pine. (Refs. 25).

1981-02-01

202

The Isolation and Characterization of d-Glucose 6-Phosphate Cycloaldolase (NAD-Dependent) from Acer pseudoplatanus L. Cell Cultures  

UK PubMed Central (United Kingdom)

A soluble enzyme system from suspension cultures of Acer pseudoplatanus L. converts d-glucose 6-phosphate to myoinositol. A Mg2+-dependent phosphatase, present in...Full Text Available

1971-09-01

203

Regulation of Indole-3-Acetic Acid Biosynthetic Pathways in Carrot Cell Cultures 1  

UK PubMed Central (United Kingdom)

2,4-Dichlorophenoxyacetic acid (2,4-D) promotes the accumulation of tryptophan-derived indole-3-acetic acid (IAA) in carrot cell cultures during callus proliferation by a biosynthetic pathway that is...Full Text Available

1992-11-01

204

Plant cell engineering: current research, application and future prospects  

International Nuclear Information System (INIS)

This paper reviewed the current status of basic research in plant cell engineering, highlighted the application of embryo culture, double haploid (DH) technology, protoplast culture and somatic hybridization, somaclonal variation, rapid propagation, and bio-products production of plant-origin, and t he prospects. (authors)

2008-10-01

205

Inhibitory effect of transforming growth factor-. beta. (TGF-. beta. ) on insulin-like growth factor 1 (IGF-1)-induced proliferation and differentiation in primary cultures of pig preadipocytes  

Energy Technology Data Exchange (ETDEWEB)

The influence of serum, IGF-1 and TGF-{beta} on the differentiation of preadipocytes was examined in primary cultures of porcine adipose tissue cells. In serum-supplemented or serum-free, IGF-1 (1 and 10 nM) had no effect on total cell number. However, IGF-1 (10nM) increased adipocyte number only in serum-supplemented (1% pig serum) cultures, whereas TGF-{beta} (15 pm) reduced the adipocyte number in the presence and absence of IGF-1. Replication of preadipocytes was analyzed with a ({sup 3}H) thymidine assay. Preadipocyte proliferation (cpm in adipocyte fraction) was increased by IGF-1 (10nM) only in cultures containing pig serum. TGF-{beta} had no effect on preadipocyte proliferation specifically, but slightly increased total ({sup 3}H) thymidine incorporation in cultures with serum. Glycerol phosphate dehydrogenase (GPDH) specific activity was decreased by adding TGF-{beta} to serum-free ...

1990-02-26

206

Inhibitory effect of transforming growth factor-#beta# (TGF-#beta#) on insulin-like growth factor 1 (IGF-1)-induced proliferation and differentiation in primary cultures of pig preadipocytes  

International Nuclear Information System (INIS)

The influence of serum, IGF-1 and TGF-#beta# on the differentiation of preadipocytes was examined in primary cultures of porcine adipose tissue cells. In serum-supplemented or serum-free, IGF-1 (1 and 10 nM) had no effect on total cell number. However, IGF-1 (10nM) increased adipocyte number only in serum-supplemented (1% pig serum) cultures, whereas TGF-#beta# (15 pm) reduced the adipocyte number in the presence and absence of IGF-1. Replication of preadipocytes was analyzed with a ["3H] thymidine assay. Preadipocyte proliferation (cpm in adipocyte fraction) was increased by IGF-1 (10nM) only in cultures containing pig serum. TGF-#beta# had no effect on preadipocyte proliferation specifically, but slightly increased total ["3H] thymidine incorporation in cultures with serum. Glycerol phosphate dehydrogenase (GPDH) specific activity was decreased by adding TGF-#beta# to serum-free ...

1991-04-21

207

Evaluation of the Bactec MGIT 960 system in combination with the MGIT TBc identification test for detection of Mycobacterium tuberculosis complex in respiratory specimens.  

Science.gov (United States)

The sensitivity and specificity of the MGIT TBc identification (TBc ID) test for Mycobacterium tuberculosis complex (MTC) detection in positive Bactec MGIT cultures were 95.2% and 99.2%, respectively. When MTC-positive results obtained from two additional molecular methods were included, the sensitivity of the MGIT TBc ID test was 85.4%, while that of culture was 95.7%. PMID:21450949

2011-03-30

208

Effect of Elicitation and Changes in Extracellular pH on the Cytoplasmic and Vacuolar pH of Suspension-Cultured Soybean Cells 1  

UK PubMed Central (United Kingdom)

We have employed both 31P nuclear magnetic resonance spectroscopy and two intracellular fluorescent pH indicator dyes to monitor the pH of the vacuole and cytoplasm of suspension-cultured...Full Text Available

1992-02-01

209

Control mechanisms operating for lipid biosynthesis differ in oil-palm (Elaeis guineensis Jacq.) and olive (Olea europaea L.) callus cultures.  

UK PubMed Central (United Kingdom)

As a prelude to detailed flux control analysis of lipid synthesis in plants, we have examined the latter in tissue cultures from two important oil crops, olive (Olea europaea L.) and oil palm (Elaeis...Full Text Available

2002-06-01

210

Irradiation as an alternative post harvest treatment  

International Nuclear Information System (INIS)

This current world population has significantly added to the pressures placed upon our finite resources and our resulting ability to feed ourselves. In order to cope with current and future demands, the two established lines of action, that is, reduced population growth and expansion of agricultural production, must be supplemented with the parallel activity of reducing food losses during and after harvest. For developing countries in particular, enormous post-harvest losses result from spillage, contamination, pests and physiological deterioration during storage. Studies in these countries indicate that post-harvest losses are enormous and amount to tens of millions of tons per year valued at billions of dollars. Programs to reduce post-harvest losses, if applied properly, can result in realistic yield increases between 10 and 30%, which can be directly converted into increased consumption for humans. Post-harvest losses vary greatly and are a function of the crop variety, pest ...

1997-10-27

211

Soil less culture; I sistemi di coltivazione senza suolo  

Energy Technology Data Exchange (ETDEWEB)

The paper gives a general view of techniques and systems related to soil less culture developed in the last years (on substrate in beg; NFT; Ebb-Flood, aeroponic,..) taking into account their management and problems (water quality, control of plant nutrition and irrigation; substrates; pathological aspects,..). The evolution, now in progress, of soil less culture from open to closed system as a way to realized an environmental friendly growing system, is considered. When plants are grown with open cycle techniques a large amount of waste solution, with an a high content of nutrients, are discharged in soil and water. Furthermore, they need an extra-utilization of water and fertilizers. Another aspect is the utilization of low cost substrates, which can be reused for more than one cultural cycle without negative effects on yield, and also finally discharged without negative effects on the environment. The development of soil ...

1996-01-01

212

Atrial natriuretic peptide receptor heterogeneity and effects on cyclic GMP accumulation  

Energy Technology Data Exchange (ETDEWEB)

The effects of atrial natriuretic peptide (ANP), oxytocin (OT) and vasopressin (AVP) on guanylate cyclase activity and cyclic GMP accumulation were examined, since these hormones appear to be intimately associated with blood pressure and intravascular volume homeostasis. ANP was found to increase cyclic GMP accumulation in ten cell culture systems, which were derived from blood vessels, adrenal cortex, kidney, lung, testes and mammary gland. ANP receptors were characterized in intact cultured cells using {sup 125}I-ANP{sub 8-33}. Specific {sup 125}I-ANP binding was saturable and of high affinity. Scratchard analysis of the binding data for all cell types exhibited a straight line, indicating that these cells possessed a single class of binding sites. Despite the presence of linear Scatchard plots, these studies demonstrated that cultured cells possess two functionally and physically distinct ANP-binding sites. Most of the ...

1988-01-01

213

The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors  

Energy Technology Data Exchange (ETDEWEB)

An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface ...

2008-10-15

214

Online quasi-continuous measurement of organic acids in the atmosphere  

Energy Technology Data Exchange (ETDEWEB)

The concentration of some organic acids in the atmosphere was determined using a wet effluent diffusion denuder-aerosol collector coupled with Ion Chromatography. Three organic acids and four inorganic anions were identified and quantified in the air sample, taken from the backyard of PSI. (author)

2002-03-01

215

NAME=\\  

Wastenet

...In your area Get connected Farmers & growers news Training & events Poultry (laying) Poultry production can be a profitable enterprise for organic farmers and eggs are ... To find out more about organic poultry production download our free comprehensive introductory guide: Organic poultry production - an introductory guide [PDF, ...

216

Studies on the appearance of skeletal anomalies in red porgy: effect of culture intensiveness, feeding habits and nutritional quality of live preys  

British Library Electronic Table of Contents (United Kingdom)

Summary Despite the great interest of red porgy as a new species for Mediterranean aquaculture, its commercial production is constrained by the high incidence of skeletal deformities occurring in this species under culture conditions. Several studies have been conducted to better understand the origin of these anomalies in this species, using different system intensiveness, rotifers enrichment products or rotifers docosahexaenoic acid content. The first study showed that culture intensification increased the number of fish with an extra vertebrae, what was probably related to the different nutritional quality of live preys employed in each treatment, since water temperature, salinity and genetic background were identical for the different batches of fish studied. Total incidence of skeleta...

2010-01-01

217

Social involvement and development as a response to the campus student culture  

British Library Electronic Table of Contents (United Kingdom)

Given the widely accepted notion of whole person education in Confucian societies such as Hong Kong, Mainland China and Singapore, it is surprising that research literature originated in these societies pays little attention to how students learn and develop through out-of-class experiences at university. There is little research evidence on how the prevailing culture among student social communities (residential halls and student societies/clubs) influences students? social involvement and development. This paper examines 42 Chinese students? social experiences and development during their freshman year at a Hong Kong university. The majority of them were intensively involved in out-of-class activities. Their active social involvement was both a response to the culture of student communit...

2011-01-01

218

Politics, guanxi and the search for objectivity: the intricacies of conducting educational research in Chinese contexts  

British Library Electronic Table of Contents (United Kingdom)

This article discusses how history, the cultural setting, and the political-ideological contexts may influence educational research in China. It seeks to demonstrate a dichotomy between official and popular discourses, and argues that there is a need for the researcher to understand and interpret the language style used in various interview settings and research publications in China. Further, it is contended that ideology and cultural influences push towards a 'virtuous' or socially acceptable understanding of reality. These ideological and cultural norms may also affect official research data and statistics. Further, the article seeks to demonstrate that for a researcher in China (whether Chinese or not) it is important to cultivate and make use of guanxi (connections), at the same time ...

2011-01-01

219

Patterns of proliferation and differentiation of irradiated haemopoietic stem cells cultured on normal 'stromal' cell colonies in vitro  

Energy Technology Data Exchange (ETDEWEB)

Experiments were designed to elucidate whether or not the irradiated bone marrow cells receive any stimulation for the self-replication and differentiation from normal 'stromal' cell colonies in the bone marrow cell culture in vitro. When irradiated or unirradiated bone marrow cells were overlaid on the normal adherent cell colonies, the proliferation of haemopoietic stem cells was supported, the degree of the stimulation depending on the starting cellular concentration. There was, however, no significant changes in the concentration of either CFUs or CFUc regardless of the dose of irradiation on the bone marrow cells overlaid. This was a great contrast to the dose-dependent decrease of CFUs or CFUc within the culture in which both the stem cells and stromal cells were simultaneously irradiated. These results suggest that the balance of self-replication and differentiation of the haemopoietic stem cells is affected only when ...

1981-09-01

220

Lecturas neobarrocas del Espejo de paciencia y reconceptualizaciones de la naci?n: Jos? Lezama Lima, Cintio Vitier y Severo Sarduy  

British Library Electronic Table of Contents (United Kingdom)

This article examines the successive reinterpretations of one of Cuba?s foundational texts, namely the Espejo de paciencia (?Model of Patience??, 1608) by Silvestre de Balboa, as a baroque poem. The revalorization of the baroque by twentieth-century Cuban authors and critics has been explained as a consequence of the erasure of indigenous cultures and the subsequent imposition of a metropolitan culture. In this sense, the baroque is supposed to be paradigmatic for ?roots thinking?? about nation-building and culture. The readings of Balboa?s poem by neo-baroque writers such as Jos? Lezama Lima, Cintio Vitier and Severo Sarduy, however, put forward a transhistorical vision of the baroque and Cubanhood as an eternal and immutable phenomenon or, alternatively, a reading that privileges the tec...

2010-01-01

221

Environmental, scanning electron and optical microscope image analysis software for determining volume and occupied area of solid-state fermentation fungal cultures  

British Library Electronic Table of Contents (United Kingdom)

Abstract Here we propose a software for the estimation of the occupied area and volume of fungal cultures. This software was developed using a Matlab platform and allows analysis of high-definition images from optical, electronic or atomic force microscopes. In a first step, a single hypha grown on potato dextrose agar was monitored using optical microscopy to estimate the change in occupied area and volume. Weight measurements were carried out to compare them with the estimated volume, revealing a slight difference of less than 1.5%. Similarly, samples from two different solid-state fermentation cultures were analyzed using images from a scanning electron microscope (SEM) and an environmental SEM (ESEM). Occupied area and volume were calculated for both samples, and the results obtained w...

2011-01-01

222

Developing defined culture systems for human pluripotent stem cells  

British Library Electronic Table of Contents (United Kingdom)

Human pluripotent stem cells hold promising potential in many therapeutics applications including regenerative medicine and drug discovery. Over the past three decades, embryonic stem cell research has illustrated that embryonic stem cells possess two important and distinct properties: the ability to continuously self-renew and the ability to differentiate into all specialized cell types. In this article, we will discuss the continuing evolution of human pluripotent stem cell culture by examining requirements needed for the maintenance of self-renewal in vitro. We will also elaborate on the future direction of the field toward generating a robust and completely defined culture system, which has brought forth collaborations amongst biologists and engineers. As human pluripotent stem cell re...

2011-01-01

223

Coloring of cultured pearls by gamma-rays irradiation  

International Nuclear Information System (INIS)

Changing cream pearls into bluish-grey by #gamma# ray irradiation is a technique in coloring of pearls. Irradiated pearls are similar in color to cultured blue pearls. The pearl layers hardly change their color but the nuclei change into dark brown by irradiation. Visible light (500 - 700 nm) penetrating the pearl layer is absorbed by dark brown nucleus. The intensity of reflecting light between 400 and 500 nm at pearl surface, therefore, becomes stronger than that between 500 and 700 nm; therefore color of irradiated pearls look bluish-grey. The density of bluish-grey color increases with increasing absorbed doses, but their luster at surface diminishes owing to the deterioration of the pearl layer by prolonged irradiation; high doses irradiation should be avoided. Irradiated pearls show no substantial fading of their color in a year and the rate of the fading is found to be lower than that for cultured blue pearls. (author).

224

Cell culture and gene transcription effects of copper sulfate on Chinese hamster ovary cells  

British Library Electronic Table of Contents (United Kingdom)

Abstract This study reports the effects of varying concentrations of copper sulfate on the metabolic and gene transcriptional profile of a recombinant Chinese hamster ovary (CHO) cell line producing an immunoglobulin G (IgG)-fusion protein (B0). Addition of 50 M copper sulfate significantly decreased lactate accumulation in the cultures while increasing viable cell density and protein titer. These changes could be seen from day 6 and became increasingly evident with culture duration. Reducing the copper sulfate concentration to 5 M retained all the above beneficial effects, but with the added benefit of reduced levels of the aggregated form of the B0 protein. To profile the cellular changes due to copper sulfate addition at the transcriptional level, Affymetrix CHO microarrays were used to...

2011-01-01

225

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1  

British Library Electronic Table of Contents (United Kingdom)

In the present study, human neonatal fibroblasts were isolated from a two-month-old human male. The purpose of the present investigation was the analysis of the morphology (light and transmission electron microscopy), karyotype and growth characteristics of the human neonatal fibroblast cell culture B-HNF-1. Moreover, STR typing and mitochondrial DNA amplification and sequencing was also performed. Analysis of chromosomes count showed that B-HNF-1 cell culture is diploid and has normal male karyotype 46, XY, which was stable during cultivation. The transmission electron microscopy demonstrated the ultra-structure of the B-HNF-1 cells; they have typical morphological features of proteosynthesis-active cells. Large number of fibroblasts bearing different shapes and surface characteristics ad...

2010-01-01

226

A cultural model of household energy consumption  

International Nuclear Information System (INIS)

In this paper, we consider the development of demand-side research, from an early interest in conservation behavior to a later focus on physical, economic, psychological and social models of energy consumption. Unfortunately, none of these models account satisfactorily for measured energy consumption in the residential sector. Growing interest in the end-uses of energy (e.g. in support of load forecasting, demand-side management and least-cost utility planning), increasing international studies of energy use, and continuing work in the energy and lifestyles research tradition now support an emerging cultural perspective on household energy use. The ecological foundations of the cultural model and its applications in energy research are discussed, along with some of the analytic consequences of this approach. (author).

227

Food and drink : Frequently asked questions : Soil Association  

Wastenet

... Do you certify Halal or Kosher meat? What fish feed do you allow? What levels of polychlorinated biphenyls (PCBs) have been found in organic fish? How can I be sure that organic products from abroad are really organic? Do you prosecute businesses that break the rules? Does the Soil Association carry out any routine testing of food? Do you test for GM material? Why do you allow additives in organic food? Is organic food fortified with vitamins and minerals? Why choose organic during pregnancy?...

228

Toxicity of Jet A (Aviation Fuel) Selected Aquatic Organisms  

Science.gov (United States)

... Descriptors : *JET ENGINE FUELS, *AQUATIC ORGANISMS, *AVIATION FUELS, *TOXICITY, HEALTH, SMOKE SCREENS, WIND, WATER ...

1989-03-01

229

Sorption of Selected Volatile Organic Constituents of Jet Fuels ...  

Science.gov (United States)

... TITLE (Include Security Classification) Sorption of Selected Volatile Organic Constituents of Jet Fuels and Solvents on Natural Sorbents from Gas ...

1988-08-01

230

Skin as an endocrine organ: implications for its function  

UK PubMed Central (United Kingdom)

Described as the body's largest organ, the skin is strategically located at the interface with the external environment where it has evolved to detect, integrate and respond to a diverse range...Full Text Available

2008-06-01

231

Preparation of covalently modified organic-inorganic composite nanoparticles and their interfacial electron transfer researches  

CERN Document Server

Preparation of covalently modified organic-inorganic composite nanoparticles and their interfacial electron transfer researches

1998-01-01

232

Pathogenesis of hypocalcemia in magnesium depletion  

UK PubMed Central (United Kingdom)

Hypocalcemia in the hypomagnesemic state in man is usually attributed to refractoriness of end-organs to the calcemic action of parathyroid hormone. We studied the responsiveness of end-organs to bovine...Full Text Available

1971-12-01

233

In-situ micro-FTIR study of thermal changes of organics in Tagish ...  

Science.gov (United States)

FT-IR spectroscopic studies of major organic matter in carbonaceous chondrites using microscopic technique and comparison with terrestrial kerogen. ...

234

Importance of health and environment as quality traits in the buying decision of organic products  

British Library Electronic Table of Contents (United Kingdom)

This paper aims to explore consumer preference for fresh vegetables labelled as organic in combination with health and environment related quality traits. The study decomposes organic farming into its main quality aspects and measures consumers' preference structure for organic, in general, and for specific organic quality traits in particular. By means of stated choice preference modelling, the following hypotheses are tested: consumers prefer health over environment related quality traits; the organic label plays a significant role in consumers' choice for organic products; organic farming is perceived as healthier and more environmentally friendly than conventional farming; purchase intention is mainly driven by health related quality traits; both health and environmental concerns influ...

2009-01-01

235

Determination of alcohols, esters and organic acids in irradiated sweet potato wine by capillary gas chromatography  

International Nuclear Information System (INIS)

This paper presents the analytical results of alcohols, esters and organic acids in sweet potato wine irradiated by #gamma#-rays. (author).

237

AFO Public Meeting -- Seattle, WA Proceedings  

Science.gov (United States)

years I chaired a national committee drafting recommended standards for organic livestock production for the Organic Trade Association and had the opportunity to work with...

2003-01-06

238

Hydration of swelling clay and bacteria interaction. An experimental in situ reaction study; Hydratation des argiles gonflantes et influence des bacteries. Etude experimentale de reaction in situ  

Energy Technology Data Exchange (ETDEWEB)

This study reports on the physical-chemical behaviour of swelling di-octahedral clays (smectites) and their interaction with aqueous solutions and bacteria (Shewanella putrefaciens). Experimental results are presented for compacted clays, hydrated under confined volume conditions, using a new type of reaction-cell (the 'wet-cell' of Warr and Hoffman, 2004) that was designed for in situ X-ray diffraction (XRD) measurement. For comparison, dispersed clay systems were studied using standard batch solutions subjected to varying degrees of agitation. The combination of time-dependent in situ XRD measurements with gravimetric measurements and calculated diffraction patterns using the CALCMIX software (Plancon and Drits, 1999) allowed to successful quantification of the dynamics of water uptake and storage. This analytical procedure combined with published water vapour adsorption data enabled determination of the abundance of structured water layers, developed in the ...

2008-01-15

239

Zinc release from thapsigargin/IP3-sensitive stores in cultured cortical neurons  

UK PubMed Central (United Kingdom)

BackgroundChanges in ionic concentration have a fundamental effect on numerous physiological processes. For example, IP3-gated thapsigargin sensitive intracellular calcium...Full Text Available

240

Why do men marry and why do they stray?  

UK PubMed Central (United Kingdom)

Humans are quite unusual compared to other great apes in that reproduction typically takes place within long-term, iteroparous pairings—social arrangements that have been culturally reified...Full Text Available

2007-07-07

241

Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)  

UK PubMed Central (United Kingdom)

BackgroundSelection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream (Sparus aurata)...Full Text Available

242

Use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of Campylobacter jejuni and Campylobacter coli.  

UK PubMed Central (United Kingdom)

A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to...Full Text Available

1990-07-01

243

Use of Forward Genetics to Discover Novel Regulators of NF-?B  

UK PubMed Central (United Kingdom)

Forward and reverse genetic experiments have both played important roles in revealing critical aspects of mammalian signal transduction pathways in cell culture experiments. Only recently have we begun...Full Text Available

2010-06-01

244

Urinary tract infection in children.  

UK PubMed Central (United Kingdom)

During 1968-77, 572 consecutive children with one or more positive urine cultures who were referred by their family doctors to one paediatric surgical outpatient clinic were investigated and prospectively...Full Text Available

1984-08-04

245

Tissue-engineered product: allogeneic cultured dermal substitute composed of spongy collagen with fibroblasts.  

Science.gov (United States)

Recently, various types of allogeneic skin substitutes including cultured epidermal substitute (CES), cultured dermal substitute (CDS), and cultured skin substitute (CSS), which are composed of keratinocytes and/or fibroblasts as the cellular component(s), have been used as biological wound dressings. In our study, the allogeneic CDS was prepared by plating fibroblasts on a spongy collagen. The clinical evaluation was conducted using fresh or cryopreserved allogeneic CDS. In 145 of our clinical cases, 95% (138/145) of various wounds were evaluated as achieving good or excellent results, including 96% (22/23) of deep dermal burns (DDB) and dermal burns (DB), 100% (53/53) of partial-thickness donor wounds, 91% (21/23) of traumatic skin defects, 100% (5/5) of pressure ulcers, 82% (9/11) of chronic skin ulcers, 100% (6/6) of coverage for debrided DB, and 92% (22/24) of coverage for autologous meshed graft. The results obtained ...

2001-03-01

246

Tick-Borne Relapsing Fever in British Columbia, Canada: First Isolation of Borrelia hermsii  

UK PubMed Central (United Kingdom)

The spirochete that causes tick-borne relapsing fever, Borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern...Full Text Available

1998-12-01

247

Three-dimensional culture models of mammary gland  

UK PubMed Central (United Kingdom)

The mammary gland is a complex tissue comprised of a branching network of ducts embedded within an adipocyte-rich stroma. The ductal epithelium is a bi-layer of luminal and myoepithelial cells, the...Full Text Available

2009-04-01

248

The protective antigens of equine herpesvirus type 1.  

UK PubMed Central (United Kingdom)

Equine herpesvirus type 1 was cultivated in swine testis cell cultures and partially purified by differential centrifugation and centrifugation in a linear sucrose density gradient. The viral envelope...Full Text Available

1978-04-01

249

The Biosynthesis of ?-Aminolevulinic Acid in Chlorella1  

UK PubMed Central (United Kingdom)

When autotrophically growing cultures of Chlorella are treated with levulinic acid, δ-aminolevulinic acid is excreted into the medium, providing a direct demonstration of α-aminolevulinic...Full Text Available

1970-04-01

250

Sugarcane pests and their management  

Science.gov (United States)

This book chapter discusses sugarcane culture and history, describes arthropod biologies and injury, and identifies sugarcane pest management factors to consider for people interested in commercial sugarcane production. Arthropod groups include 10 orders and 40 families. Sugarcane pest management ...

251

Successful establishment of primary small airway cell cultures in human lung transplantation  

UK PubMed Central (United Kingdom)

BackgroundThe study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway function...Full Text Available

2009-01-01

252

Stabilization of lignin peroxidases in white rot fungi by tryptophan.  

UK PubMed Central (United Kingdom)

Supplementation of various cultures of white rot fungi with tryptophan was found to have a large stimulatory effect on lignin peroxidase activity levels. This enhancement was greater than that observed...Full Text Available

1997-07-01

253

Stability of Trichomonas vaginalis DNA in Urine Specimens?  

UK PubMed Central (United Kingdom)

Trichomonas vaginalis is an important pathogen in both men and women. Culture is considered the diagnostic gold standard, although studies have shown that PCR is more sensitive than...Full Text Available

2008-05-01

254

Spoligotype Profile of Mycobacterium tuberculosis Complex Strains from HIV-Positive and -Negative Patients in Nigeria: a Comparative Analysis ?  

UK PubMed Central (United Kingdom)

We ran a comparative analysis of all patients for whom a positive culture of Mycobacterium tuberculosis complex was available between April 2004 and October 2005 and whose HIV serology...Full Text Available

2011-01-01

255

Sources of salmonellae in an uninfected commercially-processed broiler flock.  

UK PubMed Central (United Kingdom)

Cultural monitoring was used to study the incidence and sources of salmonellae in a 4160 bird broiler flock during the growing period, transport and processing in a commercial plant. No salmonellae...Full Text Available

1980-07-01

256

Some characteristics of a secreted chlamydial antigen recognized by IgG from C. trachomatis patient sera.  

UK PubMed Central (United Kingdom)

Chlamydia trachomatis serovars release a glycolipid antigen (GLXA) into the culture supernatant during the infective cycle. This antigen is recognized by IgG isolated from humans with a natural chlamydial...Full Text Available

1989-12-01

257

Self-management among Patients Living with Diabetes in the United States Virgin Islands  

UK PubMed Central (United Kingdom)

The United States Virgin Islands (USVI) is facing a diabetes epidemic similar to the one on the U.S. mainland, yet little is known regarding the cultural context relevant to self-management...Full Text Available

2011-01-01

258

Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.  

UK PubMed Central (United Kingdom)

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein...Full Text Available

1992-07-01

259

Role-Reversal Exercise with Deaf Strong Hospital to Teach Communication Competency and Cultural Awareness  

UK PubMed Central (United Kingdom)

ObjectiveTo implement a role-reversal exercise to increase first-year pharmacy students' awareness of communication barriers in the health care setting, especially for deaf and hard-of-hearing...Full Text Available

2011-04-11

260

Response to a trial of physician-based inpatient order entry.  

UK PubMed Central (United Kingdom)

Our group has developed a physician-operated inpatient order-entry system (BICS-OE). Mindful of the problems inherent in bringing a radical cultural change such as this to the hospital, we conducted...Full Text Available

1993-01-01

261

Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy  

UK PubMed Central (United Kingdom)

BackgroundThe circadian rhythm in mammals is orchestrated by a central pacemaker in the brain, but most peripheral tissues contain their own intrinsic circadian oscillators. The...Full Text Available

262

Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui.  

UK PubMed Central (United Kingdom)

An enzyme active against carboxymethyl cellulose (CMC) was purified from the stationary-phase-culture supernatant of Clostridium josui grown in a medium containing ball-milled cellulose. The purification...Full Text Available

1989-07-01

263

Psychometric Properties of the KPAS in Diverse Ethnic Groups of Midlife Women  

UK PubMed Central (United Kingdom)

Although the Kaiser Physical Activity Survey (KPAS) was a potential instrument for cross cultural research of midlife women, little information is available on its reliability and validity among...Full Text Available

2009-12-01

264

Proteome of human colon cancer stem cells: A comparative analysis  

UK PubMed Central (United Kingdom)

AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.METHODS: SW1116 cells were isolated and cultured with...Full Text Available

2011-03-14

265

Production of placental alkaline phosphatase (PLAP) and PLAP-like material by epithelial germ cell and non-germ cell tumours in vitro.  

UK PubMed Central (United Kingdom)

Placental and placental-like alkaline phosphatase (PLAP) levels in the culture media of 87 cell lines of neoplastic and 'normal' origin were measured by a conventional immunosorbent enzymatic assay...Full Text Available

1994-02-01

266

Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran  

British Library Electronic Table of Contents (United Kingdom)

Solid-state fermentation of soybean hulls supplemented with wheat bran using a co-culture of Trichoderma reesei and Aspergillus oryzae was performed. Three parameters - initial moisture content, incubation temperature, and initial pH - were optimized in culture flasks using response surface methodology. Parameter optimization was carried out with respect to filter paper activity and b-glucosidase activity in the culture. Temperature of 30^oC, pH of 5, and moisture content of 70% were found to be optimum. Optimized parameters were used for laboratory scale-up in static tray fermenters. The maximum filter paper activity of 10.7FPU/g-ds and b-glucosidase of 10.7IU/g-ds were obtained after 96-h incubation period in static tray fermenters in agreement with optimized activities at shake flask le...

2010-01-01

267

Product toxicity and cometabolic competitive inhibition modeling of chloroform and trichloroethylene transformation by methanotrophic resting cells.  

UK PubMed Central (United Kingdom)

The rate and capacity for chloroform (CF) and trichloroethylene (TCE) transformation by a mixed methanotrophic culture of resting cells (no exogenous energy source) and formate-fed cells were measured....Full Text Available

1991-04-01

268

Plasmid Transfer between Bacillus thuringiensis subsp. israelensis Strains in Laboratory Culture, River Water, and Dipteran Larvae  

UK PubMed Central (United Kingdom)

Plasmid transfer between strains of Bacillus thuringiensis subsp. israelensis was studied under a range of environmentally relevant laboratory conditions in vitro,...Full Text Available

2001-01-01

269

Phytochelatin Synthesis and Glutathione Levels in Response to Heavy Metals in Tomato Cells 1  

UK PubMed Central (United Kingdom)

Cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, produce phytochelatins (poly[γ-glutamylcysteinyl]glycines) when exposed to cadmium. The synthesis...Full Text Available

1987-12-01

270

Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes  

UK PubMed Central (United Kingdom)

The intercalated disk (ID) is a complex structure that electromechanically couples adjoining cardiac myocytes into a functional syncitium. The integrity of the disk is essential for normal cardiac function,...Full Text Available

2010-01-01

271

Oil palm (Elaeis guineensis Jacq.) tissue culture ESTs: Identifying genes associated with callogenesis and embryogenesis  

UK PubMed Central (United Kingdom)

BackgroundOil palm (Elaeis guineensis Jacq.) is one of the most important oil bearing crops in the world. However, genetic improvement of oil palm through conventional...Full Text Available

272

Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation.  

UK PubMed Central (United Kingdom)

A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its...Full Text Available

1975-03-01

273

Mortality, Recruitment and Change of Desert Tree Populations in a Hyper-Arid Environment  

UK PubMed Central (United Kingdom)

BackgroundLong-term vegetation changes in hyper-arid areas have long been neglected. Mortality, recruitment and change in populations of the ecologically and culturally important...Full Text Available

274

Modeling the emergence of universality in color naming patterns  

UK PubMed Central (United Kingdom)

The empirical evidence that human color categorization exhibits some universal patterns beyond superficial discrepancies across different cultures is a major breakthrough in cognitive science. As observed...Full Text Available

2010-02-09

275

Microbial transformation of artificial estrogens of the allenolic group.  

UK PubMed Central (United Kingdom)

When 2,2-dimethyl 3-(2'-naphthyl 6'-hydroxy) pentanoic acid, an artificial estrogen of the allenolic acid group, was added to an exponential-phase growth culture of Neurospora crassa (in Horowitz medium),...Full Text Available

1975-06-01

276

Mesophyll Cell Protoplasts of Potato  

UK PubMed Central (United Kingdom)

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among...Full Text Available

1977-08-01

277

Medical disclosure and refugees. Telling bad news to Ethiopian patients.  

UK PubMed Central (United Kingdom)

The strong value in American medical practice placed on the disclosure of terminal illness conflicts with the cultural beliefs of many recent refugees and immigrants to the United States, who often...Full Text Available

1992-09-01

278

Mapping the cellular and molecular heterogeneity of normal and malignant breast tissues and cultured cell lines  

UK PubMed Central (United Kingdom)

IntroductionNormal and neoplastic breast tissues are comprised of heterogeneous populations of epithelial cells exhibiting various degrees of maturation and differentiation. While...Full Text Available

2010-01-01

279

Mapping the Spatiotemporal Dynamics of Calcium Signaling in Cellular Neural Networks Using Optical Flow  

UK PubMed Central (United Kingdom)

An optical flow gradient algorithm was applied to spontaneously forming networks of neurons and glia in culture imaged by fluorescence optical microscopy in order to map functional calcium signaling...Full Text Available

2010-08-01

280

Light and Dark Controls of Nitrate Reduction in Wheat (Triticum aestivum L.) Protoplasts 1  

UK PubMed Central (United Kingdom)

Protoplasts were isolated from the leaves of nitrate-cultured wheat (Triticum aestivum L. var. Frederick) seedlings. When incubated in the dark, protoplasts accumulated nitrite under...Full Text Available

1982-02-01

281

Isolation of gram-positive rods that resemble but are clearly distinct from Actinomyces pyogenes from mixed wound infections.  

UK PubMed Central (United Kingdom)

Beginning in 1990, gram-positive rods resembling Actinomyces pyogenes were found with increasing frequency in mixed cultures from various infectious processes, most of them from patients with otitis,...Full Text Available

1993-05-01

282

Isolation and Characterization of an H2-Oxidizing Thermophilic Methanogen  

UK PubMed Central (United Kingdom)

A thermophilic methanogen was isolated from enrichment cultures originally inoculated with sludge from an anaerobic kelp digester (55°C). This isolate exhibited a temperature optimum of 55 to...Full Text Available

1983-01-01

283

Intra- and extracellular calcium modulates stereocilia stiffness on chick cochlear hair cells.  

UK PubMed Central (United Kingdom)

Segments of the chick basilar papilla were isolated and maintained in culture medium. The sensory hair bundle of individual hair cells was observed with light microscopy and stimulated with a water...Full Text Available

1994-02-01

284

Interface of culture, insecurity and HIV and AIDS: Lessons from displaced communities in Pader District, Northern Uganda  

UK PubMed Central (United Kingdom)

BackgroundNorthern Uganda unlike other rural regions has registered high HIV prevalence rates comparable to those of urbanized Kampala and the central region. This could be due to...Full Text Available

285

Inoculation of Scytalidium thermophilum in Button Mushroom Compost and Its Effect on Yield  

UK PubMed Central (United Kingdom)

Scytalidium thermophilum isolates in culture, as well as the endogenous strain(s) in mushroom compost, were inactivated at 70°C. This temperature was used to pasteurize composts...Full Text Available

1994-09-01

286

Increase of unsaturated fatty acids under high pressure in a deep-sea bacterium; Shinkai kara bunrisareta taiatsusei biseibutsu DSS12 kabu no baiyo ondo, baiyo atsuryoku to kintai shibosan sosei no henka  

Energy Technology Data Exchange (ETDEWEB)

A cellular membrane exchanges substances into and from the cell and protects the cell interior by maintaining semi-fluidity rather than be being solid. It is known that microorganisms maintain the fluidity in correspondence with varying environmental temperatures by changing and adjusting the composition of fatty acids which constitute the cellular membrane lipids. As part of the studies to elucidate the pressure withstanding mechanism in deep-sea bacteria, this paper investigates what variations the fatty acid composition, which constitutes cellular membranes of the DSS12 strain, a pressure withstanding bacterium growing well under either normal pressure or high pressures, will show under different culturing temperatures and pressures. Culture under low temperatures and culture under high pressures increase content of unsaturated fatty acids including icosapentanoic acid. Culture under high ...

1997-03-01

287

In vitro studies on the adjuvanticity of Brucella fractions.  

UK PubMed Central (United Kingdom)

Two Brucella fractions, the murein-linked fraction PI and the murein-free fraction SF, behave as in vitro adjuvants for primary anti-sheep erythrocyte responses: added to Mishell and Dutton-type cultures...Full Text Available

1982-11-01

288

Immunoquantitative Real-Time PCR for Detection and Quantification of Staphylococcus aureus Enterotoxin B in Foods  

UK PubMed Central (United Kingdom)

A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay...Full Text Available

2006-10-01

289

Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture  

UK PubMed Central (United Kingdom)

During a study on the mTor pathway in the rat kidney we observed a striking increase of the phosphorylation of the S6 kinase in mitosis. In cryostat sections of perfusion-fixed tissue mitotic cells...Full Text Available

2007-02-01

290

Hexadecane mineralization in oxygen-controlled sediment-seawater cultivations with autochthonous microorganisms.  

UK PubMed Central (United Kingdom)

Laboratory studies investigated the influence of dissolved oxygen tension (DOT) on microbial degradation of hexadecane in cultures with sediment-seawater suspensions. With a fermentor system, it was...Full Text Available

1992-09-01

291

H-1152 Effects on Intraocular Pressure and Trabecular Meshwork Morphology of Rat Eyes  

UK PubMed Central (United Kingdom)

AbstractPurposeThe aim of this study was to elucidate the effects of the Rho-kinase inhibitor, H-1152, on cultured human trabecular meshwork (HTM) cells, TM morphology,...Full Text Available

2008-08-01

292

Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy  

UK PubMed Central (United Kingdom)

This work reports the design of and experimentation with a topographically patterned cell culture substrate of variable local density and anisotropy as a facile and efficient platform to guide...Full Text Available

2009-02-06

293

Germline mutagenesis mediated by Sleeping Beauty transposon system in mice  

UK PubMed Central (United Kingdom)

Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice....Full Text Available

2007-01-01

294

Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc  

UK PubMed Central (United Kingdom)

Zinc is an essential trace metal ion for growth, but an excess of Zn is toxic and microorganisms express diverse resistance mechanisms. To understand global bacterial responses to excess Zn, we conducted...Full Text Available

2005-02-01

295

Gendered Technoculture: Sex, Lies, Videotape & Cyberspace  

Science.gov (United States)

What happens in the matrix of gender, technology, representation, and culture? This class will explore the theoretical issues raised within this matrix. We will also make sense of the theories through practical experiences with technologies like computers and cameras.

2004-02-01

296

Flow Cytometry of Human Primary Epidermal and Follicular Keratinocytes  

UK PubMed Central (United Kingdom)

Objective: The aim of this study was to characterize using flow cytometry cultured human primary keratinocytes isolated from the epidermis and hair follicles by different methods. Methods:...Full Text Available

297

Flock infection and transport as sources of salmonellae in broiler chickens and carcasses.  

UK PubMed Central (United Kingdom)

Cultural monitoring was used to determine the incidence and sources of salmonellae in a 4160-bird broiler flock raised on litter in 32 pens. Twenty-five of the pens remained apparently free of salmonellae...Full Text Available

1980-07-01

298

First Culture Isolation of Borrelia lonestari, Putative Agent of Southern Tick-Associated Rash Illness  

UK PubMed Central (United Kingdom)

Southern tick-associated rash illness (STARI) is a Lyme disease-like infection described in patients in the southeastern and south-central United States, where classic Lyme disease is relatively rare....Full Text Available

2004-03-01

299

Evidence that cell surface heparan sulfate is involved in the high affinity thrombin binding to cultured porcine aortic endothelial cells.  

UK PubMed Central (United Kingdom)

It has been postulated that thrombin binds to endothelial cells through, at least in part, cell surface glycosaminoglycans such as heparan sulfate, which could serve as antithrombin cofactor on the...Full Text Available

1985-04-01

300

Evidence That Two Major Replicons Comprise the Genome of Staphylococcus Aureus.  

Science.gov (United States)

In Staphylococcus aureus, a pronounced shift in position of the acriflavin resistance locus was observed when gene order was determined by marker frequency analysis of cells of various ages. In young cells (2-hour culture), acriflavin resistance was mappe...

1967-01-01

301

Evaluation of the Widal tube agglutination test for the diagnosis of typhoid fever among children admitted to a rural hdospital in Tanzania and a comparison with previous studies  

UK PubMed Central (United Kingdom)

BackgroundThe diagnosis of typhoid fever is confirmed by culture of Salmonella enterica serotype Typhi (S. typhi). However, a more rapid, simpler,...Full Text Available

302

Epidermal keratinocytes do not activate peripheral T-cells: interleukin-10 as a possible regulator  

UK PubMed Central (United Kingdom)

The immunogenicity of allogeneic cultured human epidermal keratinocytes (cHEKs) has been studied in several models with contradictory results. We studied human T-cell activation in an in vitro...Full Text Available

2008-11-01

303

Enzyme amplified immunoassay: a novel technique applied to direct detection of Chlamydia trachomatis in clinical specimens.  

UK PubMed Central (United Kingdom)

Endocervical swabs from 212 women and urethral swabs from 100 men were tested by the routine methods for McCoy cell culture and simultaneously by a novel enzyme amplified immunoassay test to detect...Full Text Available

1985-10-01

304

Endometrial biopsy in Holstein-Friesian dairy cows. III. Bacteriological analysis and correlations with histological findings.  

UK PubMed Central (United Kingdom)

This study examines the results of bacterial culture from 159 endometrial biopsy samples from 97 commercial dairy cows and correlations between bacteriological and histological findings. Bacteria were...Full Text Available

1991-04-01

305

Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii?  

UK PubMed Central (United Kingdom)

Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR....Full Text Available

2009-06-01

306

Development of rat CA1 neurones in acute Versus organotypic slices: role of experience in synaptic morphology and activity  

UK PubMed Central (United Kingdom)

Despite their wide use, the physiological relevance of organotypic slices remains controversial. Such cultures are prepared at 5 days postnatal. Although some local circuitry remains intact, they develop...Full Text Available

2003-07-01

307

Detection of cellular responses to toxicants by dielectrophoresis  

UK PubMed Central (United Kingdom)

The dielectrophoretic (DEP) crossover method has been applied to the detection of cell responses to toxicants. Time and dose responses of the human cultured leukemia (HL-60) line were measured...Full Text Available

2002-08-31

308

Detection of Unculturable Bacteria in Periodontal Health and Disease by PCR  

UK PubMed Central (United Kingdom)

Recently developed molecular methods have made it possible to characterize mixed microflora in their entirety, including the substantial numbers of bacteria which do not grow on artificial culture media....Full Text Available

1999-05-01

309

Detection of Chlamydia trachomatis in genital swabs: comparison of commercial and in house amplification methods with culture  

UK PubMed Central (United Kingdom)

AIMS: To evaluate the sensitivity of the Roche Cobas, Roche Amplicor plate kit, ligase chain reaction (LCR), and an in house polymerase chain reaction (PCR) by titration of purified elementary bodies...Full Text Available

1998-08-01

310

Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.  

UK PubMed Central (United Kingdom)

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures...Full Text Available

1991-07-01

311

Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions  

British Library Electronic Table of Contents (United Kingdom)

In the present study mixed cultures that could grew in the molasses media were isolated from textile dye effluent and its decolorization activity was studied in a batch system under anaerobic conditions, in order to determine the optimal conditions required for the highest decolorization activity. The optimum pH value for decolorization was determined as 8 for all the dyes tested. In the experiment with pH 8 dye decolorizations by mixed cultures were investigated at about 96.2-1031.3mgl-1 initial dye concentrations. The highest dye removal rates of mixed cultures were 94.9% for Reactive Red RB, 91.0% for Reactive Black B and 63.6% for Remazol Blue at 953.2, 864.9 and 1031.3mgl-1 initial dye concentrations respectively within 24h incubation period. When the Reactive Red RB was used, approxi...

2006-01-01

312

Cytoplasmic heat shock granules are formed from precursor particles and are associated with a specific set of mRNAs.  

UK PubMed Central (United Kingdom)

In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger...Full Text Available

1989-03-01

313

Cytokinins and Flower Bud Formation in Vitro in Tobacco  

UK PubMed Central (United Kingdom)

Explants from flower stalks of Nicotiana tabacum L. were cultured on different cytokinins to induce flower bud formation. All cytokinins tested except zeatin and zeatin-riboside induced...Full Text Available

1990-03-01

314

Cytochrome c-d regulates developmental apoptosis in the Drosophila retina  

UK PubMed Central (United Kingdom)

The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological...Full Text Available

2006-09-01

315

Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator  

UK PubMed Central (United Kingdom)

Curcumin is a natural product currently in human clinical trials for a variety of neoplastic, preneoplastic, and inflammatory conditions. We previously observed that, in cultured cells, curcumin exhibits...Full Text Available

2009-01-08

316

Corticosteroid can alter antigen expression on alveolar macrophages.  

UK PubMed Central (United Kingdom)

Normal healthy volunteers underwent broncho-alveolar lavage and the cells obtained were cultured for 24 h and 48 h, either alone or in the presence of the corticosteroid, Budesonide. Cell differentials...Full Text Available

1991-09-01

317

Comparative Transcriptional and Genomic Analysis of Plasmodium falciparum Field Isolates  

UK PubMed Central (United Kingdom)

Mechanisms for differential regulation of gene expression may underlie much of the phenotypic variation and adaptability of malaria parasites. Here we describe transcriptional variation among culture-adapted...Full Text Available

2009-10-01

318

Cloning and expression of the gene for the Avi-3 antigen of Mycobacterium avium and mapping of its epitopes.  

UK PubMed Central (United Kingdom)

The Avi-3 antigen, which is found only in Mycobacterium avium culture sonic extracts, is species specific and results in strong skin test activity in guinea pigs sensitized with heat-killed M. avium....Full Text Available

1992-03-01

319

Charge compensation for NADPH oxidase activity in microglia in rat brain slices does not involve a proton current  

UK PubMed Central (United Kingdom)

The membrane properties of isolated cultured microglia have been extensively studied but it is important to understand their properties in situ, where they protect the brain against...Full Text Available

2008-09-01

320

Characterization of Cytokinin and Adenine Transport in Arabidopsis Cell Cultures1[OA  

UK PubMed Central (United Kingdom)

Cytokinins are distributed through the vascular system and trigger responses of target cells via receptor-mediated signal transduction. Perception and transduction of the signal can occur at the plasma...Full Text Available

2008-12-01

321

Cell resilience in species lifespans: a link to inflammation?  

UK PubMed Central (United Kingdom)

Species differences in lifespan have been attributed to cellular survival during various stressors, designated here as ‘cell resilience’. In primary fibroblast cultures, cell...Full Text Available

2010-08-01

322

Biosynthetic Pathway of Insect Juvenile Hormone III in Cell Suspension Cultures of the Sedge Cyperus iria1  

UK PubMed Central (United Kingdom)

In most insect species, juvenile hormones regulate critical physiological processes such as metamorphosis and reproduction. In insects, these sesquiterpenoids are synthesized by retrocerebral endocrine...Full Text Available

2001-10-01

323

Biological, Life Course, and Cross-Cultural Studies All point Toward the Value of Dimensional and Developmental Ratings in the Classification of Psychosis  

UK PubMed Central (United Kingdom)

The diagnostic criteria for schizophrenia in the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV1)...Full Text Available

2007-07-01

324

Biological Response Modifier Activity of an Exopolysaccharide from Paenibacillus jamilae CP-7  

UK PubMed Central (United Kingdom)

An extracellular polysaccharide was purified from culture supernatants of Paenibacillus jamilae CP-7, a gram-positive bacillus that was isolated from compost prepared with olive mill...Full Text Available

2001-07-01

325

Attachment Stimulates Exopolysaccharide Synthesis by a Bacterium  

UK PubMed Central (United Kingdom)

This study examined the hypothesis that solid surfaces may stimulate attached bacteria to produce exopolymers. Addition of sand to shake-flask cultures seemed to induce exopolymer synthesis by a number...Full Text Available

1993-10-01

326

Apoptosis of human seminoma cells upon disruption of their microenvironment.  

UK PubMed Central (United Kingdom)

One of the main obstacles encountered when trying to culture human seminoma (SE) cells in vitro is massive degeneration of the tumour cells. We investigated whether dissociation of tumour tissue, to...Full Text Available

1996-05-01

327

Albumin interacts specifically with a 60-kDa microvascular endothelial glycoprotein.  

UK PubMed Central (United Kingdom)

Confluent monolayers of microvascular endothelial cells, derived from the rat epididymal fat pad and grown in culture, were radioiodinated by using the lactoper-oxidase method. Their radioiodinated...Full Text Available

1988-09-01

328

A proteomic study of cMyc improvement of CHO culture  

UK PubMed Central (United Kingdom)

BackgroundThe biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number resulting in a maximum volumetric recombinant...Full Text Available

329

A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells  

UK PubMed Central (United Kingdom)

Cultured mouse or human embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Chemical or insertional...Full Text Available

2009-04-01

330

A convenient and sensitive allergy test: IgE crosslinking-induced luciferase expression in cultured mast cells  

UK PubMed Central (United Kingdom)

BackgroundFor the detection of allergen-specific IgE in sera, solid-phase IgE-binding assays like the CAP test are commonly used. Although such immunochemical methods are very sensitive,...Full Text Available

2010-10-01

331

A Nitrate Reductase-less Variant Isolated from Suspension Cultures of Datura innoxia (Mill.) 1  

UK PubMed Central (United Kingdom)

A comparative study has been carried out of the growth of two lines of Datura innoxia (Mill.) cells, designated DI-6 and NR1, their resistance to chlorate, and their ability to assimilate...Full Text Available

1980-10-01

332

3D Hepatic Cultures Simultaneously Maintain Primary Hepatocyte and Liver Sinusoidal Endothelial Cell Phenotypes  

UK PubMed Central (United Kingdom)

Developing in vitro engineered hepatic tissues that exhibit stable phenotype is a major challenge in the field of hepatic tissue engineering. However, the rapid dedifferentiation of...Full Text Available

333

The Integration of Energy Efficiency, Renewable Energy, DemandResponse and Climate Change: Challenges and Opportunities for Evaluatorsand Planners  

Energy Technology Data Exchange (ETDEWEB)

This paper explores the feasibility of integrating energyefficiency program evaluation with the emerging need for the evaluationof programs from different "energy cultures" (demand response, renewableenergy, and climate change). The paper reviews key features andinformation needs of the energy cultures and critically reviews theopportunities and challenges associated with integrating these withenergy efficiency program evaluation. There is a need to integrate thedifferent policy arenas where energy efficiency, demand response, andclimate change programs are developed, and there are positive signs thatthis integration is starting to occur.

2007-05-29

334

Man-made disasters: A cross-national analysis  

British Library Electronic Table of Contents (United Kingdom)

This research investigates the impact of national culture and several institutional factors on the safety performance of society and establishes statistically significant relationships between those variables. As expected, the research results reveal that some cultural variables such as uncertainty avoidance, gender orientation and institutional variables such as the degree of law avoidance can directly influence the safety performance of the society. The findings also support the inverted u-curve (Safety Kuznet curve) hypothesis indicating even if we expect a negative trend at the beginning stage of industrialization, we can expect a positive trend in safety performance as their income level continues to improve beyond a certain point.

2011-01-01

335

In vitro study of plutonium in macrophages  

International Nuclear Information System (INIS)

An in vitro system for studying Pu uptake by, and removal from, macrophages was developed to provide data for the actinide therapy program. We have shown that "2"3"9PuO_2 particle uptake in vitro resembles the process in vivo with respect to the intracellular localization of phagocytized particles. At the end of 7 days of culture of Pu-loaded cells, 54% of the cells were viable even though 71% of the cells in the culture initially contained enough "2"3"9PuO_2 to deliver more than 53 intracellular #alpha#-disintegrations.

1977-05-01

336

Determination of the cell and mucous distribution in the airways of the lung  

International Nuclear Information System (INIS)

Models of the human airways have played a major role in evaluating the health effects of inhaled radionuclides. While models such as those of Weibel (1963) provide data necessary for characterizing deposition of aerosol, they have not characterized the cells at risks in the airspaces. Given the advancements in techniques and study of cell cultures exposed to ionizing radiation there is a need to extrapolate between the simple structures of cell culture systems and the complex architecture of the human airways. The preliminary data in this paper provide a complete characterization of the size and number of cells in the airways and represents a significant advance in our study of the health consequences of exposure to inhaled radionuclides. 26 refs., 2 figs., 4 tabs.

1991-01-01

337

Tests for the diagnosis of Clostridium difficile infection: the next generation.  

Science.gov (United States)

Clostridium difficile (C. difficile) causes 25-30% of cases of antibiotic associated diarrhea and most cases of pseudomembranous colitis. Patients presenting with diarrhea after hospitalization for 3 or more days should be tested for C. difficile. There are many options available for testing, each of which has inherent advantages and disadvantages. Most laboratories perform toxin testing using an enzyme immunoassay method. In general these tests have sensitivities ranging from 60 to 70% and specificities of 98%. When using these methods, symptomatic patients with negative tests should be tested by another more sensitive method. Until recently, cell culture cytotoxicity neutralization assays (CCNAs) were considered the gold standard in the U.S. A two-step algorithm using an EIA for glutamate dehydrogenase detection followed by testing positives using CCNA, offered an improved alternative until the availability of molecular assays. Although early studies that ...

2011-03-03

338

Regulation of sulfated glycosaminoglycan production by prostaglandin E2 in cultured lung fibroblasts  

Energy Technology Data Exchange (ETDEWEB)

Prostaglandin E2 (PGE2) has been shown to increase the synthesis of hyaluronic acid in cultured fibroblasts by increasing the activity of hyaluronate synthetase, a group of plasma membrane-bound synthetic enzymes. We examined whether PGE2 also increased the activity of those enzyme systems involved in the synthesis of sulfated glycosaminoglycan in the human embryonic lung fibroblast. Exposure of cells to PGE2 resulted in dose-dependent increases in glucosamine incorporation into all sulfated glycosaminoglycan subtypes. PGE2 at 10(-7) mol/L increased total glycosaminoglycan per dish to 21.6 +/- 3.1 micrograms versus 12.0 +/- 2.5 micrograms in control untreated cultures. Stimulation of endogenous PGE2 production by bradykinin had a similar effect on glycosaminoglycan synthesis. To examine whether PGE2 affected sulfated glycosaminoglycan protein core production, cells were labeled with tritiated glucosamine in the presence of cycloheximide. Under ...

1989-08-01

339

Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations  

Science.gov (United States)

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured human hepatoma cells, but save for the genotype 2a isolate JFH-1, efficient replication of these HCV RNAs requires replication enhancing mutations (REMs), previously also called cell culture adaptive mutations. These mutations cluster primarily in the central region of non-structural protein 5A (NS5A), but may also reside in the NS3 helicase domain or at a distinct position in NS4B. Most efficient replication has been achieved by combining REMs residing in NS3 with distinct REMs located in NS4B or NS5A. However, in spite of efficient replication of HCV genomes containing such mutations, they do not support production of infectious virus particles. By using the genotype 1b isolate Con1, in this study we show that REMs interfere with HCV ...

2009-06-12

340

Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells  

Energy Technology Data Exchange (ETDEWEB)

The origin of the epithelial and myoepithelial cells in the human breast has not been delineated. In this study we have addressed whether luminal epithelial cells and myoepithelial cells are vertically connected, i.e., whether one is the precursor for the other. We used a primary culture assay allowing preservation of basic phenotypic traits of luminal epithelial and myoepithelial cells in culture. The two cell types were then separated immunomagnetically using antibodies directed against lineage-specific cell surface antigens into at best 100% purity. The cellular identity was ascertained by cytochemistry, immunoblotting, and 2-D gel electrophoresis. Luminal epithelial cells were identified by strong expression of cytokeratins 18 and 19 while myoepithelial cells were recognized by expression of vimentin and {alpha}-smooth muscle actin. We used a previously devised culture medium (CDM4) that allows vigorous expansion of ...

1999-02-01

341

Growth-related variations in the glycosaminoglycan synthesis of ultraviolet light-induced murine cutaneous fibrosarcoma cells  

Energy Technology Data Exchange (ETDEWEB)

Glycosaminoglycan synthesis was studied in cell populations of ultraviolet light-induced murine cutaneous fibrosarcoma cells under conditions of varying growth rates in vitro. After labeling with the precursors, /sup 3/H-glucosamine and /sup 35/SO/sub 4/, sulfated glycosaminoglycans recoverable by direct proteolysis of the culture monolayers increased approximately 5-fold on a per cell basis from sparsely populated, exponential cell cultures (greater than 85% of cells in S, G2, or M phases) to stationary cultures inhibited by high cell density (greater than 50% of cells in G1). Within this cell surface-associated material, the relative ratio of heparan sulfate to the chondroitin sulfates was approximately 60/40% under conditions of exponential growth; in the growth-arrested cultures, the reverse ratio was found. The substratum attached material, obtained from the flask surface after ethyl glycol ...

1985-08-01

343

Some comments on BEIR III  

International Nuclear Information System (INIS)

... organizations irradiation radiation doses radiation effects RADIATIONS.

1982-01-01

344

Situation with Rabies in the Republic of Tajikistan  

International Science & Technology Center (ISTC)

Assessment of the Situation with Rabies in the Republic of Tajikistan and Organization of Activities on Rabies Control

346
348

Organic Manure and Crop Organic Carbon Returns - Effects on Soil Quality (Soil-QC)  

Environmental Research Database

DescriptionThe overall objective of the project is to provide an improved understanding of the processes and linkages through which organic carbon additions influence soil bio-physical and physico-chemical properties. Soil organic carbon (SOC) levels and turnover rates are intimately linked to the soil properties that are important in the maintenance of soil quality and fertility, and sustainable crop production. However, it has been difficult to distinguish the various processes and linkages through which [continued...

2009-01-31

349

NAME=\\  

Wastenet

...Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain Assemblages of plants and invertebrate animals of tumulus ...organic mound) springs of the Swan Coastal Plain [Skip navigation links] About us | Contact us | Publications |...ecological communities Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain Advice to the Minister for the ...of the 'Assemblages of plants and invertebrate animals of tumulus (organic mound) springs of the Swan Coastal Plain' community sufficient to distinguish it from ...

350

Methods and results of gas chromatographicmass spectrometric determination of volatile organic substances in an urban atmosphere  

Energy Technology Data Exchange (ETDEWEB)

The method and results of the quantitative gas chromatographic determination of volatile organic substances in an urban atmosphere are presented. The concentration of organic substances was determined by using a mixed adsorber consisting of graphitized thermal carbon black and activated charcoal modified by pyrocarbon. Average, maximum and minimum concentrations of constant organic components in the atmosphere of Leningrad as a typical large industrial city are reported.

1983-01-01

351

Maintenance viewpoint of a successful reactor program  

International Nuclear Information System (INIS)

As the Operating and Support staffs of the FFTF organization have gained experience, the plant reliability and capacity factors have shown a steadily improving trend. The plant capacity factor for Cycle 4 was 99.5%. It is the purpose of this report to describe the evolution of the maintenance organization at the FFTF site from a general support organization to a technically proficient organization playing a major role in planning and performance of plant maintenance evolutions.

1984-06-03

352

Luminescence of guest - host type organic nanostructures  

International Nuclear Information System (INIS)

... Abstract only 1063-7869 v. 44(10) CLASSICAL AND QUANTUM MECHANICS,

2001-10-31

354
358

Contribution to the radiation preparation of wood-plastic materials. Pt. 7  

International Nuclear Information System (INIS)

... odd nuclei organic compounds radiation effects radioisotopes synthesis

1974-01-01

359

Breath Test for Chemicals (Volatile Organic Compounds)  

Science.gov (United States)

Breath Tests; Human Volunteers; Pilot Study

2011-09-16

363

Institute of Toxicology. 1994 annual report on research and development; Institut fuer Toxikologie. Ergebnisbericht ueber Forschung und Entwicklung 1994  

Energy Technology Data Exchange (ETDEWEB)

The R and D activities of the institute focused on two major projects, ``Molecular and cellular environmental toxicology``, and ``Biophysics of multi-cellular systems``. For assessment of health risks emanating from the uptake of heavy metal compounds, work performed under the first mentioned project studies the formation of allergy-inducing metabolic products from membrane lipids, and the effects of related cellular signaling processes. These studies are accompanied by an approach to quantify and classify the toxicity of environmental organometallic compounds. Another major task is research into the pathogenesis of lung disease induced by dusts, gases, and gas-dust mixtures and the liberation of inflammatory agents by immunological cell reactions (alveolar macrophages). Atomic force microscopy is the tool used to visualize the related dynamic changes in bronchial cells. Studies for detection of the localisation and structure of the Fe-Mo co-factor in nitrogenase by means of the method ...

1995-12-31

364

Organic against inorganic electrodes grown onto polymer substrates for flexible organic electronics applications  

Energy Technology Data Exchange (ETDEWEB)

One of the most challenging topics in the area of organic electronic devices is the growth of transparent electrodes onto flexible polymeric substrates that will be characterized by enhanced conductivity in combination with high optical transparency. An essential aspect for these materials is their synthesis and/or microstructure which define the transparency, the stability and the interfacial chemistry which in turn determine the performance and stability of the organic electronic devices, such as organic light emitting diodes, organic photovoltaics, etc. In this work, we will discuss the latest advances in the growth of organic (e.g. PEDOT:PSS) and inorganic (e.g. zinc oxide-ZnO, indium tin oxide-ITO) conductive materials and their deposition onto flexible polymeric substrates. We will compare the optical, structural, nano-mechanical and nano-topographical properties of the ...

2009-12-15

365

The SPOOM-EDM method for assessing organizational factors  

International Nuclear Information System (INIS)

Organization factors have been known as an important contributor to plant safety. Previous studies associated with assessing organisation factors mainly deals with the aspect of safety of an organization. For an organization, however, efficiency or an aspect of economy related with work activities is also important. This paper introduces a conceptual model, SPOOM-EDM (Self Poly-Oriented Organizational Model - Evaluation Diamond Model), for evaluating an organization with respect to both safety and economy. It also shows how the proposed model can be applied for the evaluation of an organization through the analysis of real events. (author)

2003-04-20

366

Mathematical child phantom for the calculation of dose to the organs at risk  

Energy Technology Data Exchange (ETDEWEB)

In order to calculate the doses received by the organs of 530 children treated by radiation for cancer between 1945 and 1969 at the G. Roussy Institute, we have developed a computer program for organ location calculation. To calculate the location of each child's organs of interest at the time of the treatment, only two parameters are necessary; sex and height or sex and age when the height at the time of the treatment is unknown. The algorithm is based on the metric studies of growth known as auxology. Each organ is located by one point representing its center. The model has been checked on 100 healthy children.

1988-05-01

367

Organic livestock production in Uganda: potentials, challenges and prospects.  

Science.gov (United States)

Development in organic farming has been stimulated by farmers and consumers becoming interested in healthy food products and sustainable environment. Organic agriculture is a holistic production management system which is based on the principles of health, ecology, care, and fairness. Organic development in Uganda has focused more on the crop sector than livestock sector and has primarily involved the private sector, like organic products export companies and non-governmental organizations. Agriculture in Uganda and many African countries is predominantly traditional, less mechanized, and is usually associated with minimum use of chemical fertilizers, pesticides, and drugs. This low external input agriculture also referred to as "organic by default" can create basis for organic farming where agroecological methods are introduced and present ...

2011-01-12

368

Use of Organic Acids to Inactivate-Escherichia coli-O157:H7,-Salmonella-Typhimurium, and-Listeria monocytogenes-on Organic Fresh Apples and Lettuce  

British Library Electronic Table of Contents (United Kingdom)

Abstract:- This study was undertaken to investigate the antimicrobial effect of organic acids against-Escherichia coli-O157:H7,-Salmonella-Typhimurium, and-Listeria monocytogenes-on whole red organic apples and lettuce. Several studies have been conducted to evaluate organic acids as sanitizers. However, no studies have compared antimicrobial effects of various organic acids on organic fresh produce, including evaluation of color changes of produce. Apples and lettuce were inoculated with a cocktail of 3 strains each of 3 foodborne pathogens provided above and treated with 1% and 2% organic acids (propionic, acetic, lactic, malic, and citric acid) for 0, 0.5, 1, 5, and 10 min. With increasing treatment time and acid concentration, organic acid treatments showed significant reduction compar...

2011-01-01

369

Geochemical and petrographic properties of some Spitsbergen coals and dispersed organic matter  

Energy Technology Data Exchange (ETDEWEB)

This paper presents the characteristics of selected parameters of organic matter of the Tertiary coal samples and organic matter of Carboniferous rock samples from the Spitsbergen. The coal samples were taken from Central Coal Basin (the Longyearbyen region) and from the Forlandsundet Basin (Oscar II Land, the Kaffioyra region). Samples of dispersed organic matter were collected from Suffolk Pynten and Sergeijevfjellet area in Sorkapp Land. The optical properties of coal samples are different from properties of dispersed organic matter. Macerals of vitrinite group dominate in all of the samples. The average content of vitrinite group macerals is much lower in dipersed organic matter samples than it is in coals. The average content of liptinite group macerals is a little lower, and inertinite group macerals is much higher. The average content of mineral matter is higher in ...

2004-02-20

370

Regulation of the phosphoinositide pathway in cultured Sertoli cells from immature rats: effects of follicle-stimulating hormone and fluoride  

International Nuclear Information System (INIS)

Many hormones elicit effects on target cells by stimulating the enzyme phospholipase-C, which catalyzes the hydrolysis of phosphoinositides to the intracellular second messengers diacylglycerol and inositol phosphates. The present study examined the roles of FSH and guanine nucleotide-binding proteins (G-proteins) in regulating the hydrolysis of phosphoinositides in Sertoli cells. Sertoli cell cultures prepared from 16- to 18-day-old rats were incubated for 24 h with myo-[2-3H] inositol to label endogenous phospholipids. Treatment of cells from 0.5-20 min with preparations of ovine FSH ranging in potency from 1-60 times that of NIH FSH S1 did not affect accumulation of inositol phosphates. Levels of total [3H]inositol phosphates [[3H]inositol mono-, di-, and triphosphates (IP, IP2, and IP3)] in FSH-treated cultures was 75-120% the levels in control cultures over the various time intervals studied. Addition of testosterone ...

371

Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes  

Energy Technology Data Exchange (ETDEWEB)

To study the regulation of adipose tissue lipoprotein lipase (LPL) in human adipocytes, omental adipose tissue was obtained from healthy subjects and digested in collagenase. The isolated adipocytes thus obtained were suspended in Medium 199 and cultured at 37 degrees C. Cell viability was demonstrated in adipocytes cultured for up to 72 h by constancy of cell number, cell size, trypan-blue exclusion, and specific /sup 125/I-insulin binding. In addition, chloroquine induced an increase in cell-associated /sup 125/I-insulin at 24, 48, and 72 h after preparation. Thus, isolated adipocytes retained their ability to bind, internalize, and degrade insulin. LPL was measured as activity secreted into the culture medium (CM), released from cells by heparin (HR), and extracted from cell digests. A broad range of heparin concentrations produced a prompt release of LPL from a rapidly replenishable pool of cellular activity. When cells ...

1985-01-01

372

Production of Shiga-like toxins by Escherichia coli O157:H7 can be influenced by the neuroendocrine hormone norepinephrine.  

Science.gov (United States)

To examine whether the neuroendocrine hormone norepinephrine may influence the production of the Shiga-like toxins (SLTs), several Escherichia coli O157:H7 clinical isolates were grown in the presence or absence of norepinephrine. An in vitro culture system consisting of low (<1500 colony-forming units/ml) initial concentrations of inocula into a serum-based medium was used to more closely approximate in vivo conditions. The growth of all isolates was increased several logs in the presence of norepinephrine, as compared with the growth in controls, during a 24-hour growth period. Controls included additional dextrose as well as the use of the norepinephrine metabolite normetanephrine, which contains one more methyl group than norepinephrine and hence would serve as a better energy source for growth if the effect were solely nutritionally mediated. During the 24 hours of growth, the production of cell-associated SLT-I on a protein-equivalent basis was shown to be ...

1996-10-01

373

Effect of temperature on the development of Steinernema carpocapsae and Steinernema feltiae (Nematoda: Rhabditida) in liquid culture.  

Science.gov (United States)

For commercial use of the entomopathogenic nematodes Steinernema carpocapsae and Steinernema feltiae in biological control of insect pests, they are produced in liquid culture on artificial media pre-incubated with their symbiotic bacteria Xenorhabdus nematophila and Xenorhabdus bovienii, respectively. After 1 day of the bacterial culture, nematode dauer juveniles (DJs) are inoculated, which recover development. The adult nematodes produce DJ offspring, which are harvested and can be sprayed. This study determined optimal temperatures to obtain high DJ progeny within a short process time. Temperatures assessed were 23 degrees C, 25 degrees C, 27 degrees C, and 29 degrees C for S. carpocapsae and 20 degrees C, 23 degrees C, 25 degrees C, and 27 degrees C for S. feltiae. The recovery of inoculated DJs was hardly affected and was reduced only in S. carpocapsae at 29 degrees C. The fecundity (eggs in uterus) in S. carpocapsae reached a maximum at ...

2009-05-20

374

Cultured epidermal allografts as biological wound dressings.  

Science.gov (United States)

Recent advances in cell culture technology permit the generation of large stratified epithelial sheets appropriate for wound coverage. Autografts (sheets prepared from the patient's own skin) have proven life-saving in the treatment of large third-degree burns and have been successfully employed in the management of chronic ulcers. Allografts (sheets prepared from the skin of an unrelated donor) have also been used. In our experience, cultured allografts derived from neonatal foreskin provide a potent stimulus to healing in a variety of partial thickness wounds. Their application is a simple outpatient procedure which involves no discomfort for the patient. In contrast to autografting, no biopsy is necessary and use of cultured allogenic cells permits immediate grafts availability and possibility of stockpiling and preserving grafts for future use. Preparation of epithelial sheets suitable for grafting is also faster and ...

1991-01-01

375

Geochemical features of re-deposited organic matter occurring in fluvioglacial sediments in the Racib?rz region (Poland): A case study  

British Library Electronic Table of Contents (United Kingdom)

The erosion of rocks rich in organic matter typically leads to the complete mineralization of the organic material. However, in some cases, it is re-deposited to become a part of sediments once more. This process should be considered to be a part of global carbon cycle, possibly much more significant than assumed to-date. The research presented here aims to characterize re-worked organic matter occurring in post-glacial sediments of southern part of Poland, in the Oder river valley (the Racib?rz town region, Miocene, Pleistocene and Holocene age). Organic substances extracted from the sediments originated from organic matter that had resided in rocks eroded by glaciers. Sediments were sampled in two boreholes which sediments were correlated. Sediments were extracted and extracts analyzed w...

2008-01-01

376

[Aeromonas hydrophila-related septicemia in the Nile tilapia Oreochromis niloticus].  

Science.gov (United States)

From diseased wild and cultured Oreochromis niloticus in Lower Egypt, 17 Aeromonas hydrophila isolates were recovered. The mortality was between 10% and 70% in among cultured fish. The course of the disease ran in an acute manner. For cultured fish, the disease outbreaks were found mainly in winter and for the wild Nile fish, mortalities were observed in late spring and summer. Additionally wild fish were affected with ectoparasites. The LD50 values of the isolates ranged between 10(3) and 10(7). Isolates of high virulence were resistant to 1 hr boiling and to the bactericidal effect of fresh normal guinea pig serum. Moreover, they did not agglutinate in acriflavin. Only the virulent isolates could agglutinate tilapia erythrocytes. The above effects were reversed for avirulent isolates while moderately virulent isolates showed no consistency in their reactions. Tube agglutination test using O and WC antisera prepared ...

1989-03-01

377

Statistical optimization of biohydrogen production from sucrose by a co-culture of Clostridium acidisoli and Rhodobacter sphaeroides  

Energy Technology Data Exchange (ETDEWEB)

Statistically based experimental designs were applied to optimize the fermentation process parameters for hydrogen (H{sub 2}) production by co-culture of Clostridium acidisoli and Rhodobacter sphaeroides with sucrose as substrate. An initial screening using the Plackett-Burman design identified three factors that significantly influenced H{sub 2} yield: sucrose concentration, initial pH, and inoculum ratio. These factors were considered to have simultaneous and interdependent effects. A central composite design and response surface analysis were adopted to further investigate the mutual interactions among the factors and to identify the values that maximized H{sub 2} production. The optimal substrate concentration, initial pH, and inoculum ratio of C. acidisoli to R. sphaeroides were 11.43 g/L sucrose, 7.13, and 0.83, respectively. Using these optimal culture conditions, substrate conversion efficiency was determined as 10.16 mol H{sub 2}/mol ...

2010-05-15

378

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation  

British Library Electronic Table of Contents (United Kingdom)

Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24?h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western bl...

2008-01-01

379

Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn.  

British Library Electronic Table of Contents (United Kingdom)

Callus cultures of Calophyllum inophyllum were established using seed, nodal/ internodal and leaf explants on WPM basal medium supplemented with indole-3-butyric acid (IBA), a-naphthalene acetic acid (NAA), picloram (4-amino-3,5,6-trichloropicolinic acid), and 6-benzylaminopurine (BAP) in different combinations and concentrations with the view to study the influence of hormones on callus induction and the pattern of expression of dipyranocoumarins including anti-HIV, non-nucleoside reverse transcriptase inhibitors inophyllum B and P in callus cultures. 96.01% seed explants, 87.50% nodal/internodal explants and 86.66% leaf explants were converted into calluses when inoculated on WPM supplemented with IBA 4.0mgl-1 along with BAP 1.0mgl-1, IBA 4.0mgl-1, and picloram 6.0mgl-1 along with BAP 2....

2007-01-01

380

Optimization of culture conditions in CO2 fixation for succinic acid production using Actinobacillus succinogenes  

British Library Electronic Table of Contents (United Kingdom)

The culture conditions in CO2 fixation by Actinobacillus succinogenes for succinic acid production were investigated by a model of available CO2 in a 3-l fermentor. The results from the model analysis showed that the available CO2 for succinic acid production in the fermentation broth is the sum of HCO3 ?, CO3 2?, and CO2 influenced by external culture conditions such as medium components, CO2 partial pressures, and temperature. The optimized conditions for CO2 supply in a 3-l fermentor were determined as follows: CO2 partial pressure and stirring speed were maintained at 0.1?MPa and 200?r?min?1, respectively, with a pH of 6.8 and a temperature of 37?C; 0.15?mol?l?1 NaHCO3 was added. Under the optimized conditions, a CO2 fixation rate of 0.57?g?l?1?h?1 was obtained, and a succinic acid con...

2011-01-01

381

Improvement of banana through biotechnology and mutation breeding  

International Nuclear Information System (INIS)

Protocols were standardized for in vitro propagation of several elite and diverse banana accessions using shoot tip explants. Tissue culture raised plants were field planted at multiple locations. Studies were undertaken for the induction of mutations using multiple shoot cultures of six selected cultivars, Shreemanti (AAA), Basrai (AAA), Lal Kela (AAA), Rasthali (AAB), Karibale Monthan (ABB) and a wild diploid (BB). These shoot cultures were irradiated at different doses of gamma rays (0-100 Gy) and subcultured thrice (up to M_1V_3) to separate shimeras, followed by induction of rooting (M_1V_4). In general, the rate of multiplication had a negative association with the dose of gamma rays. Enhanced multiplication of shoots was noticed at lower doses. The proliferation of shoots was arrested beyond 50 Gy and a dose of 70 Gy was completely lethal for all the genotypes studied. The rooted plantlets were hardened in the green ...

1998-10-01

382

Genotoxic effects of sunlight-activated waste waters  

Energy Technology Data Exchange (ETDEWEB)

Natural sunlight induces a genotoxic response in cultured CHO cells pre-treated with shale oil retort process water. Near ultraviolet light (NUV) component of the solar spectrum is the apparent radiation responsible for photoactivation. Cultured human skin fibroblasts are acutely sensitive to the genotoxic effects of photoactivated process water. The mutagenic potential of photoactivated process water in human cells is the same as that witnessed for an equivalent killing dose of the potent skin carcinogen FUV. DNA repair processes are involved in modulating genotoxic effects of this photo-induced process. The exact magnitude of the potential health-related and environmental risks resulting from photoactivation of retort process waters and other oil shale by-products is unassessed at this time. Our demonstration that a significant rate of mutation occurs in cultured human cells exposed to high dilutions of process waters and ...

1981-01-01

383

Examination of thermally polluted water for free living amoebae and testing for their possible pathogenic properties  

Energy Technology Data Exchange (ETDEWEB)

Water and mud samples were collected from canals and rivers which were adjacent to outlets discharging warm water of 3 power plants in Berlin. Downstream samples from 1 bathing resort were also collected. Free living amoebae were isolated from 138 water and 69 mud samples. From these respectively 156 and 73 strains could be cultured and were administered intranasally to mice for pathogenicity tests. Two Acanthamoeba strains from water and 7 from mud could be reisolated from mouse brain and or lungs, although no pathological disorders could be observed. Five Naegleria strains were negative in mouse inoculation tests. Four Acanthamoeba strains which were positive in mice were cultured at + 45 degrees C; no cytopathogenic effects were observed in tissue cultures. Acanthamoeba infective for mice could also be isolated from samples at low water temperatures. Further investigations have to show, whether changes in virulence of ...

1982-05-01

384

Elimination of a new ampelovirus (GLRaV-Pr) and Grapevine rupestris stem pitting associated virus (GRSPaV) from two Vitis vinifera cultivars combining in vitro thermotherapy with shoot tip culture  

British Library Electronic Table of Contents (United Kingdom)

A new virus species designated as Grapevine leafroll associated virus-Pr (GLRaV-Pr), which is classified in a distinct phylogenetic group of the genus Ampelovirus (Closteroviridae), was recently characterized from Greek grapevine cultivars. Elimination studies of GLRaV-Pr were carried out in two grapevine cultivars, 'Mantilaria' and 'Prevezaniko', co-infected with Grapevine rupestris stem pitting associated virus (GRSPaV, Flexiviridae). Both viruses were detected by nested RT-PCR assays. Virus elimination was achieved by combining in vitro thermotherapy with meristem ( 0.2mm) or shoot tip culture ( 0.5cm). The survival and regeneration rate of meristems was very low. On the other hand, high survival rates were observed in the cultured shoot tips accompanied with high elimination rates for ...

2009-01-01

385

Effect of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Nanofiber Matrices Cocultured With Hair Follicular Epithelial and Dermal Cells for Biological Wound Dressing  

British Library Electronic Table of Contents (United Kingdom)

Abstract: We tested the effects on the early-stage wound healing of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber matrices cultured with hair follicular cells. PHBV only, PHBV/collagen, and PHBV/gelatin at a 7/3 weight ratio were produced by electrospinning, and their in vitro cell culture and in vivo wound healing as biological dressings were examined. In cell attachment and growth on matrices, dermal sheath (DS) cells attached to hydrophilic PHBV/collagen and PHBV/gelatin faster than hydrophobic PHBV at the early incubation stage (up to 6 h). From 6- to 24-h incubation, PHBV/collagen showed the best results in cell culture. Furthermore, PHBV/collagen cocultured for 3-5 days with DS and epithelial outer root sheath (ORS) cells expressed more extracellular materials, such a...

2007-01-01

386

Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad  

British Library Electronic Table of Contents (United Kingdom)

Abstract The objective of this study was to determine the functional properties of cartilaginous tissues generated by porcine MSCs isolated from different tissue sources, and to compare these properties to those derived from chondrocytes (CCs). MSCs were isolated from bone marrow (BM) and infrapatellar fat pad (FP), while CCs were harvested from the articular surface of the femoro-patellar joint. Culture-expanded CCs and MSCs were encapsulated in agarose hydrogels and cultured in the presence of TGF3. Samples were analysed biomechanically, biochemically and histologically at days 0, 21 and 42. After 42 days in free swelling culture, mean GAG content was 1.50% w/w in CC-seeded constructs, compared to 0.95% w/w in FP- and 0.43% w/w in BM-seeded constructs. Total collagen accumulation was hig...

2011-01-01

387

Cellulolytic Enzymes Production via Solid-State Fermentation: Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate.  

Science.gov (United States)

We investigated the effect of pretreatment on the physicochemical characteristics-crystallinity, bed porosity, and volumetric specific surface of soybean hulls and production of cellulolytic enzymes in solid-state fermentation of Trichoderma reesei and Aspergillus oryzae cultures. Mild acid and alkali and steam pretreatments significantly increased crystallinity and bed porosity without significant change inholocellulosic composition of substrate. Crystalline and porous steam-pretreated soybean hulls inoculated with T. reesei culture had 4 filter paper units (FPU)/g-ds, 0.6?IU/g-ds ?-glucosidase, and 45?IU/g-ds endocellulase, whereas untreated hulls had 0.75?FPU/g-ds, 0.06?IU/g-ds ?-glucosidase, and 7.29?IU/g-ds endocellulase enzyme activities. In A. oryzae steam-pretreated soybean hulls had 47.10?IU/g-ds endocellulase compared to 30.82?IU/g-ds in untreated soybean hulls. Generalized linear statistical model fitted to enzyme activity data ...

2011-06-15

388

Biological conversion of synthesis gas. Limiting conditions/scale-up  

Energy Technology Data Exchange (ETDEWEB)

The purpose of this research is to develop a technically and economically feasible process for biologically producing H(sub 2) from synthesis gas while, at the same time, removing harmful sulfur gas compounds. Six major tasks are being studied: 1. Culture development, where the best cultures are selected and conditions optimized for simultaneous hydrogen production and sulfur gas removal; 2. Mass transfer and kinetic studies in which equations necessary for process design are developed; 3. Bioreactor design studies, where the cultures chosen in Task 1 are utilized in continuous reaction vessels to demonstrate process feasibility and define operating conditions; 4. Evaluation of biological synthetic gas conversion under limiting conditions in preparation for industrial demonstration studies; 5. Process scale-up where laboratory data are scaled to larger-size units in preparation for process demonstration in a pilot-scale ...

1993-09-01

389

Biohydrogen production from desugared molasses (DM) using thermophilic mixed cultures immobilized on heat treated anaerobic sludge granules  

British Library Electronic Table of Contents (United Kingdom)

Hydrogen production from desugared molasses (DM) was investigated in both batch and continuous reactors using thermophilic mixed cultures enriched from digested manure by load shock (loading with DM concentration of 50.1 g-sugar/L) to suppress methanogens. H"2 gas, free of methane, was produced during batch cultivations, at different (DM) concentrations ranging from 1.5 g-sugars/L to 50.1 g-sugars/L. The highest yield of 237 ml-H"2/g-sugar was achieved during the DM batch fermentation at concentration of 2.1 g-sugars/L, whereafter the yield decreased with increasing DM concentration. The enriched hydrogen producing mixed culture achieved from the 16.7 g-sugars/L DM batch cultivation was immobilized on heat treated anaerobic sludge granules in an up-flow anaerobic sludge blanket (UASB) reac...

2011-01-01

390

6-Methyl-1,2,4-benzenetriol, a new intermediate in penicillic acid biosynthesis in Penicillium cyclopium  

International Nuclear Information System (INIS)

Penicillic acid-negative mutants were obtained from a color mutant derived from Penicillium cyclopium NRRL 1888 through N-methyl-N'-nitro-N-nitrosoguanidine treatment. One mutant (SK2N6) accumulated 6-methyl-1,2,4-benzenetriol, which was not previously known to be a metabolite of P. cyclopium, in addition to orsellinic acid and orcinol. The radioactivity of [1-"1"4C]acetic acid was rapidly incorporated into 6-methyl-1,2,4-benzenetriol in a culture of P. cyclopium SK2N6. Moreover, the radioactivity of ["1"4C]6-methyl-1,2,4-benzenetriol was efficiently incorporated into penicillic acid in a culture of P. cyclopium NRRL 1888. These data indicate that 6-methyl-1,2,4-benzenetriol is a precursor for penicillic acid biosynthesis. The results on the addition of 1,4-dihydroxy-6-methoxy-2-methylbenzene, 6-methoxy-2-methylbenzoquinone (1,4), and 1-O-methylorcinol to a culture of P. cyclopium SK2N6 indicated that only the former two ...

391

Watchdog Calls on USDA to Boost Transparency in Organic Governance  

Wastenet

...qualified and who were under consideration at the time, USDA Secretary Tom Vilsack chose an animal husbandry specialist employed by one of the largest organic livestock product marketers in the country. While this appointee had grown up on a conventional farm, her immediate occupation is not that of ...

392

Transcriptional Organization of the Avian Adenovirus CELO  

UK PubMed Central (United Kingdom)

A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs)....Full Text Available

1998-11-01

393

Topographical Organization of Mu and Beta Band Activity Associated with Hand and Foot Movements in Patients with Perirolandic Lesions  

UK PubMed Central (United Kingdom)

To study the topographical organization of mu and beta band event-related desynchronization (ERD) associated with voluntary hand and foot movements, we used magnetoencephalographic (MEG) recordings...Full Text Available

394

The effects of thiamin on lead metabolism: organ distribution of lead 203.  

UK PubMed Central (United Kingdom)

The effect of thiamin on the organ distribution of lead was evaluated in CD-1 mice exposed intragastrically or intraperitoneally to a single dose of lead acetate (100 micrograms) containing 100 microCi...Full Text Available

1992-07-01

395

Systems-level analysis of microbial community organization through combinatorial labeling and spectral imaging  

UK PubMed Central (United Kingdom)

Microbes in nature frequently function as members of complex multitaxon communities, but the structural organization of these communities at the micrometer level is poorly understood because of limitations...Full Text Available

2011-03-08

396

Self-organization of nickel atoms in silicon  

British Library Electronic Table of Contents (United Kingdom)

We present experimental evidence for self-organization of nickel microparticles in silicon under certain thermodynamic conditions of nickel diffusion doping. The concentration and distribution of the microparticles in silicon are very uniform. Additional anneals lead to self-ordering of the impurity microparticles.

2011-01-01

397

Selective metabolic stimulation of the subfornical organ and pituitary neural lobe by peripheral angiotensin II  

Energy Technology Data Exchange (ETDEWEB)

The subfornical organ is a major receptor area for one of the principal stimuli of thirst, the octapeptide, angiotensin II. In conscious water-sated rats, the authors examined the effects of intravenous infusion of angiotensin II on the rate of glucose utilization in the subfornical organ and in structures anatomically and functionally connected with it. Angiotensin II produced pressor and drinking responses and increased glucose utilization selectively in the subfornical organ and pituitary neural lobe and in no other brain structure. Treatment with the angiotensin II antagonist, sar1-leu8-angiotensin II, before intravenous administration of angiotensin II prevented metabolic stimulation of the subfornical organ and neural lobe. Captopril, an inhibitor of angiotensin-converting enzyme, reduced subfornical organ glucose metabolism to a level similar to that found in control animals. ...

1985-01-01

398

Requirements concerning radiosterilization process organization  

International Nuclear Information System (INIS)

Administrative procedure connecting for licensing new materials or consumer products appropriated to radiosterilization have been performed and explained. Also the organization of irradiation process for attaining the proper result have been described in detail. 4 refs, 1 tab.

1997-05-26

399

Photoexcited charge pair escape and recombination  

Science.gov (United States)

Overviews, publications, and new directions are presented for the following research topics: geminate charge pairs in hexane, dipoles in nonpolar and polar organic liquids, organic donor-acceptor interfaces, and charge-transfer states (phenanthrene/PMDA).

1992-09-15

400

Organizing, Educating, and Advocating for Health and Human Rights in Vieques, Puerto Rico  

UK PubMed Central (United Kingdom)

I briefly review the process of community organization, education, and advocacy activities that ended the harmful military practices in the island-municipality of Vieques, Puerto Rico, while drawing...Full Text Available

2005-01-01

401

Organ-Specific Invertase Deficiency in the Primary Root of an Inbred Maize Line 1  

UK PubMed Central (United Kingdom)

An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble)...Full Text Available

1991-10-01

402

Nurses' knowledge, attitudes, and beliefs regarding organ and tissue donation and transplantation.  

UK PubMed Central (United Kingdom)

The acute shortage of human organs and tissues for transplantation has been attributed in part to health professionals, including nurses, for their reluctance to recognize and refer suitable candidates...Full Text Available

1991-03-01

403

NASCENT: An automatic protein interaction network generation tool for non-model organisms  

UK PubMed Central (United Kingdom)

Large quantity of reliable protein interaction data are available for model organisms in public depositories (e.g., MINT, DIP, HPRD, INTERACT). Most data correspond to experiments with the proteins...Full Text Available

404

Mechanism of atmospheric photooxidation of organic compounds. Reactions of alkoxy radicals in oxidation of n-butane and simple ketones  

Energy Technology Data Exchange (ETDEWEB)

The OH-initiated photo-oxidation of n-butane was used as a source of 1- and 2-butoxy radicals. Reactions producing ketones and other organic compounds are explained. Rates of photolysis were determined and are discussed.

1981-05-01

405

LITERATURE SURVEY OF THE CORROSION BEHAVIOR OF TANTALUM, ZIRCONIUM, AND TITANIUM  

Science.gov (United States)

The corrosion behavior of Ta, Ti, and Zr in inorganic acids, bases, chlorides and miscellaneous salts, waters and gases, and organic acids and miscellaneous organic chemicals is summarized. (W.L.H)

1955-02-23

406

Impact of the 3,6,9-Trioxadecyloxy Group on Desazadesferrithiocin Analogue Iron Clearance and Organ Distribution  

UK PubMed Central (United Kingdom)

The impact of introducing a 3,6,9-trioxadecyloxyl group at various positions of the desazadesferrithiocin (DADFT) aromatic ring on iron clearance and organ distribution is described. Three DADFT...Full Text Available

2007-07-12

407

Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.  

UK PubMed Central (United Kingdom)

Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium...Full Text Available

1990-03-01

408

Gamuts in nuclear medicine  

Energy Technology Data Exchange (ETDEWEB)

This book provides differential diagnostic possibilities of specific scan abnormalities in one volume for easy reference. This book is organized by organ systems and covers the central nervous system, the endocrine, skeletal, pulmonary, gastrointestinal, genitourinary, and cardiovascular systems. The gamuts are designed to teach differential diagnosis and to assist in the actual interpretation of patient slides.

1983-01-01

409

Embryonic anti-aging niche  

UK PubMed Central (United Kingdom)

Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. The poor performance of stem cells in an aged...Full Text Available

410

Bioaccumulation and toxicity of selenium compounds in the green alga Scenedesmus quadricauda  

UK PubMed Central (United Kingdom)

BackgroundSelenium is a trace element performing important biological functions in many organisms including humans. It usually affects organisms in a strictly dosage-dependent manner...Full Text Available

411

Basic Principles of Information Technology Organization in Health Care Institutions  

UK PubMed Central (United Kingdom)

Abstract This paper focuses on the basic principles of information technology (IT) organization within health sciences centers. The paper considers the placement of the leader of the...Full Text Available

1997-03-01

412

Advanced organic analysis and analytical methods development: FY 1995 progress report. Waste Tank Organic Safety Program  

Energy Technology Data Exchange (ETDEWEB)

This report describes the work performed during FY 1995 by Pacific Northwest Laboratory in developing and optimizing analysis techniques for identifying organics present in Hanford waste tanks. The main focus was to provide a means for rapidly obtaining the most useful information concerning the organics present in tank waste, with minimal sample handling and with minimal waste generation. One major focus has been to optimize analytical methods for organic speciation. Select methods, such as atmospheric pressure chemical ionization mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry, were developed to increase the speciation capabilities, while minimizing sample handling. A capillary electrophoresis method was developed to improve separation capabilities while minimizing additional waste generation. In addition, considerable emphasis has been placed on developing a rapid screening tool, based ...

1995-09-01

413

13th Meeting of the Scientific Group on Methodologies for the Safety Evaluation of Chemicals (SGOMSEC): alternative testing methodologies for organ toxicity.  

UK PubMed Central (United Kingdom)

In the past decade in vitro tests have been developed that represent a range of anatomic structure from perfused whole organs to subcellular fractions. To assess the use of in vitro tests for toxicity...Full Text Available

1998-04-01

414

Zebrafish embryo extracts promote sphere-forming abilities of human melanoma cell line  

British Library Electronic Table of Contents (United Kingdom)

Sphere-forming abilities in culture condition are considered a hallmark of cancer stem-like cells, which represents tumor cell invasiveness and stem-like characteristics. We aimed to show that the sphere-forming subpopulation of human malignant melanoma cell line WM-266-4 acts differently to zebrafish embryo extracts compared with their bulk counterpart. Spheres were maintained in neural stem cell culture conditions. The embryos of zebrafish at specific developmental stages were collected and the extracts were purified under 100 kDa. Spheres were treated with embyo extracts and proliferation assay and immunocytochemistry were conducted. Spheroid cells expressed nestin and epidermal growth factor receptor (EGFR) but not melanoma antigen recognized by T-cells (MART)1, indicating their stem-l...

2009-01-01

415

X-ray fluorescence in research on Czech cultural monuments  

Energy Technology Data Exchange (ETDEWEB)

Radionuclide X-ray fluorescence analysis (RXRFA), as a non-destructive method, relatively simple and allowing measurements to be carried out in situ, is an excellent tool in research on various objects of art. A range of artefacts have been investigated by our laboratory, in part for the purposes of history of art and in part as a basis for restoration works - medieval frescoes in some Czech castles and churches, metal sculptures and objects of applied art, paints and inks of old manuscripts, paintings. Some of these are among the most valuable monuments in the Czech cultural heritage. The contribution of the results of the tests to the investigation of their 'life story' and, in some cases, to their conservation, is not negligible. Later additions and repairs can be recognised, and materials and technologies that are close to their historic counterparts can be used in restoration work.

2004-01-01

416

X-ray fluorescence in research on Czech cultural monuments  

International Nuclear Information System (INIS)

Radionuclide X-ray fluorescence analysis (RXRFA), as a non-destructive method, relatively simple and allowing measurements to be carried out in situ, is an excellent tool in research on various objects of art. A range of artefacts have been investigated by our laboratory, in part for the purposes of history of art and in part as a basis for restoration works - medieval frescoes in some Czech castles and churches, metal sculptures and objects of applied art, paints and inks of old manuscripts, paintings. Some of these are among the most valuable monuments in the Czech cultural heritage. The contribution of the results of the tests to the investigation of their 'life story' and, in some cases, to their conservation, is not negligible. Later additions and repairs can be recognised, and materials and technologies that are close to their historic counterparts can be used in restoration work.

2004-01-01

417

Use of flow cytometry to compare the antimicrobial efficacy of silver-containing wound dressings against planktonic Staphylococcus aureus and Pseudomonas aeruginosa  

British Library Electronic Table of Contents (United Kingdom)

Abstract Silver-impregnated wound dressings continue to be routinely used for the management of infected wounds, or wounds that are at risk of becoming infected. The ability of antimicrobials that have been incorporated into wound dressings to kill microorganisms within the dressing requires appropriate evaluation using in vitro models. In vitro models that have been exploited for this purpose have included the corrected zone of inhibition and the log reduction assay. However, these and other related culturable-based assays are purported to have poor correlation with the overall microbicidal barrier activity of an antimicrobial wound dressing. This is because culturable-based methods only retrospectively indicate bacterial cell death and do not take into account viable but nonculturable st...

2011-01-01

418

The Relevance of Art Education and the Education of the Nigerian Child: Implications for the Universal Basic Education Policy  

Science.gov (United States)

This article examines relevant government policy documents on education and culture and discovers that Nigerian education authorities do not "discriminate" against art and culture in its articulation of educational policies per se, but lack of administrative machinery or political will has resulted in the deprivation of the Nigerian child in the process of creative activity in early childhood development. The article argues that lack of creative ability in our educational products is perhaps a major setback in the nation's quest for industrial and technological development. In this article a new art curriculum for elementary schools is advocated as a means of engaging the young child in order to attain functional educational skills necessary in the world of work.

2008-10-01

419

Terror management among Taiwanese: Worldview defence or resigning to fate?  

British Library Electronic Table of Contents (United Kingdom)

Terror management theory (TMT) proposes that people who are reminded of their mortality should be motivated to defend their cultural worldview. Studies 1 and 2 examined whether the TMT worldview defence-buffering effect found in Western cultures could be generalized to Asians in Taiwan. No such effect was found in the present studies. This non-significant result was robust when either a stronger distraction task was used (study 1) or when a subliminal manipulation of mortality salience was utilized (study 2). A meta-analysis, including 24 TMT experiments in East Asia, was also conducted (study 3). The average effect size (d = 0.11, r = 0.055) of worldview defence among these experiments was not significantly different from zero. Study 4 found that mortality salience manipulation also did n...

2010-01-01

420

Researching ethnic minority students in a Chinese context: mixed methods design for cross cultural understandings  

British Library Electronic Table of Contents (United Kingdom)

Research on and with ethnic minority students is characterised by a growing international literature that privileges 'liberal multiculturalism' as a lens through which to understand their experiences, yet ethnic diversity is constructed and responded to in many societies that are not underpinned by liberal democratic values. In this paper we use Joppke's binary to show how researching ethnic minority students outside of a liberal democratic framework requires methodologies that enable researchers to see beyond the invisibility that is often attached to ethnic minorities. We show how invisibility (i.e. antidiscrimination) can also be further culturally constructed, confounding even more any broader multicultural project. We canvass four broad areas including the role of legally binding legi...

2011-01-01

421

Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran  

British Library Electronic Table of Contents (United Kingdom)

This study investigated the occurrence of free-living amoebae (FLA) in immunodeficiency wards of hospitals in Tehran, Iran. A total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), HIV, leukemia and oncology patients of five university hospitals were collected and examined for the presence of FLA using culturing and molecular approaches. Based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of Acanthamoeba, vahlkampfiid amoebae and Hartmannella. Out of 70 samples, 37 (52.9%) were positive for FLA. Acanthamoeba belonged to the T4 genotype was the most prevalent isolate. Presence of the T4 genotype on medical instruments, including an oxygen mask in an isolation room of an immunodeficiency pediat...

2011-01-01

422

Potential Protection of Green Tea Polyphenols Against 1800?MHz Electromagnetic Radiation-Induced Injury on Rat Cortical Neurons  

British Library Electronic Table of Contents (United Kingdom)

Radiofrequency electromagnetic fields (EMF) are harmful to public health, but the certain anti-irradiation mechanism is not clear yet. The present study was performed to investigate the possible protective effects of green tea polyphenols against electromagnetic radiation-induced injury in the cultured rat cortical neurons. In this study, green tea polyphenols were used in the cultured cortical neurons exposed to 1800?MHz EMFs by the mobile phone. We found that the mobile phone irradiation for 24?h induced marked neuronal cell death in the MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide) and TUNEL (TdT mediated biotin-dUTP nicked-end labeling) assay, and protective effects of green tea polyphenols on the injured cortical neurons were demonstrated by testing the content ...

2011-01-01

423

Phytoremediation potential of Portulaca grandiflora Hook. (Moss-Rose) in degrading a sulfonated diazo reactive dye Navy Blue HE2R (Reactive Blue 172)  

British Library Electronic Table of Contents (United Kingdom)

Wild and tissue cultured plants of Portulaca grandiflora Hook. have shown to be able to decolorize a sulfonated diazo dye Navy Blue HE2R (NBHE2R) up to 98% in 40h. A significant induction in the activities of lignin peroxidase, tyrosinase and DCIP reductase was observed in the roots during dye decolorization. The wild plants and tissue cultures could independently decolorize and degrade NBHE2R into metabolites viz. N-benzylacetamide and 6-diazenyl-4-hydroxynaphthalene-2-sulfonic acid. A dye mixture and a textile effluent were also decolorized efficiently by P. grandiflora. The phytotoxicity study revealed reduction in the toxicity due to metabolites formed after dye degradation.

2011-01-01

424

Photodynamic therapy against cyanobacteria  

British Library Electronic Table of Contents (United Kingdom)

This study explores the use of photosensitizers and reactive oxygen species (ROS) to limit growth of cyanobacteria. We chose 12 phthalocyanines, tetraphenol porphyrine, and methylene blue as compounds producing singlet oxygen. Hydrogen peroxide was chosen as another source of ROS. These compounds were tested using algal toxicity tests in microplates on three cultures of green algae (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, and Chlorella kessleri) and on three cultures of cyanobacteria (Synechococcus nidulans, Microcystis incerta, and Anabaena sp.). Results indicate that photosensitizers and singlet oxygen could be highly toxic for some selected phytoplankton species. Green alga Scenedesmus quadricauda was highly sensitive (EC50 = 0.07 mg/L) to compounds producing singlet o...

2007-01-01

425

Pectinolytic yeast isolates for cold-active polygalacturonase production  

British Library Electronic Table of Contents (United Kingdom)

Pectin rich cold stored spoiled fruits, vegetables and cold soils were screened and different pectinolytic isolates were obtained by enrichment culturing and ruthenium red plate assay. Among the primary isolates 10-15% were yeast isolates. Six isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase (PGU) production at room temperature (25 degrees C) and at 5 degrees C. One isolate identified as Saccharomyces sp. with highest polygalacturonase activity at 5 degrees C was used for enzyme production using raw fruit pectins as substrates. The isolate was identified by preliminary cultural, morphological and sugar fermentation tests. PGU production was high in raw pectin substrates like orange peel (21 U/ml), apple peel (20 U/ml ), mango peel (19 U/ml), ...

2011-01-01

426

Outcome of peripherally inserted central venous catheters in surgical and medical neonates  

British Library Electronic Table of Contents (United Kingdom)

Background/Purpose: Peripherally inserted central venous catheters (PICCs) are commonly used for neonatal vascular access. The aim of this study was to look at PICC line complication rates and possible predictors of PICC infection in a neonatal intensive care unit. Method: This was a prospective study of 226 neonates who had PICCs on our neonatal intensive care unit between January 2006 and June 2009. Complete data was available on 218 neonates who had 294 PICC lines. Criteria for catheter-related sepsis was positive blood cultures (peripheral/central) and/or a positive catheter tip culture after removal in the presence of a clinical suspicion of line sepsis. Results: Of 218 neonates, 132 (169 lines) were medical, and 86 (125 lines) were surgical. Our PICC line infection rate was 17 infect...

2011-01-01

427

Nuclei with protrusions - tailed nuclei - and radiation cytogenetic markers in X-irradiated lymphocytes in vitro  

International Nuclear Information System (INIS)

The results of an investigation into tailed nuclei (TN) and features of dose dependence on frequency of the binuclear cells with TN in human lymphocyte cultures by cytogenetic block method upon X-ray radiation dose are presented. The frequencies of metaphases with dicentric and ring chromosomes are investigated concurrent with TN formed upon completion of first postradiation mitosis. Frequency of the appearance of binuclear cells with nuclei having outgrowth into the cytoplasmic space and arises after first mitosis in human lymphocyte culture is linear-square dependent on the X-irradiation at doses from 0.0 to 4.0 Gy. Positive correlation between frequency of cells with tailed nuclei and frequency of metaphases of first mitosis having dicentric and rings was established. Apparently, formation such TN is connected with dicentric and rings

428

Long-Term Follow-Up of Patients with Breast Cancer Transplanted with Autologous Ex Vivo Expanded Peripheral Blood Progenitor Cells  

British Library Electronic Table of Contents (United Kingdom)

Ex vivo expanded peripheral blood progenitor cells (PBPC) have been shown to provide rapid neutrophil engraftment, and in some patients, to eliminate neutropenia after transplantation to support high-dose chemotherapy. However, the effect of expansion culture on stem cell content and potential loss of stem cells caused by induction of differentiation remains a concern. We have transplanted 21 patients with breast cancer with expanded autologous PBPC, with 11 patients receiving expanded PBPC as their sole hematopoietic cell source. In these studies, the CD34+ cells were selected and cultured for 10 days in defined media containing 100 ng/mL each of recombinant human stem cell factor (rhSCF), recombinant human granulocyte colony stimulating factor (rhG-CSF), and recombinant human megakaryocy...

2006-01-01

429

Ionizing radiation is a potent inducer of mitotic recombination in mouse embryonic stem cells  

British Library Electronic Table of Contents (United Kingdom)

Maintenance of genomic integrity in embryonic cells is pivotal to proper embryogenesis, organogenesis and to the continuity of species. Cultured mouse embryonic stem cells (mESCs), a model for early embryonic cells, differ from cultured somatic cells in their capacity to remodel chromatin, in their repertoire of DNA repair enzymes, and in the regulation of cell cycle checkpoints. Using 129XC3HF1 mESCs heterozygous for Aprt, we characterized loss of Aprt heterozygosity after exposure to ionizing radiation. We report here that the frequency of loss of heterozygosity mutants in mESCs can be induced several hundred-fold by exposure to 5-10Gy of X-rays. This induction is 50-100-fold higher than the induction reported for mouse adult or embryonic fibroblasts. The primary mechanism underlying the...

2011-01-01

430

Investigating the biological and clinical significance of human dysbioses  

British Library Electronic Table of Contents (United Kingdom)

Culture-independent microbiological technologies that interrogate complex microbial populations without prior axenic culture, coupled with high-throughput DNA sequencing, have revolutionized the scale, speed and economics of microbial ecological studies. Their application to the medical realm has led to a highly productive merger of clinical, experimental and environmental microbiology. The functional roles played by members of the human microbiota are being actively explored through experimental manipulation of animal model systems and studies of human populations. In concert, these studies have appreciably expanded our understanding of the composition and dynamics of human-associated microbial communities (microbiota). Of note, several human diseases have been linked to alterations in th...

2011-01-01

431

Interconnected project 'Development and testing of a biological monitoring for effects of polycyclic aromatic hydrocarbons on man'  

International Nuclear Information System (INIS)

The research project had the aim to develop a standard for measuring individual, inner stresses from polycyclic aromatic hydrocarbons (PAHs) at work. The employed method was by characterizing PAHs and metabolites of PAHs to be identified in urine with a view to developing a biological monitoring of professionally exposured persons. Using a number of PAHs relevant to work, the following questions were studied in cell culture systems and different animal-experimental models: Which metabolites are formed from the PAHs used in cell culture systems and the different animal-experimental models, and what are their ratios in percent? What percentage of a PAH is excreted by experimental animals in their urine and faeces on the first, second, and third day following different forms of application? What is the scale of the individual range of variation of PAH mass excretion? Are these results transferable to man? (orig.).

1992-01-01

432

Historical Indigenous use of aquatic resources in Australia's Murray-Darling Basin, and its implications for river management  

British Library Electronic Table of Contents (United Kingdom)

Summary Indigenous people demonstrably lived along rivers and around lakes and wetlands of Australia's Murray-Darling Basin in pre-European times. Waterways were, and continue to be, of major significance to the society and culture of Aboriginal peoples throughout Australia. Historically, they exploited most of the fauna - from mussels and crayfish, to fish and birds - and either ate, or used for other purposes, many species of aquatic plants. Such practices placed them in the role of environmental modifiers, a role played by all human groups from both past and present. They built sophisticated fish traps, cut gaps in river banks to allow fish to move on to floodplains, and there is evidence that they practised a form of fish culture by creating small impoundments in which small fish could...

2007-01-01

433

High-throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts  

British Library Electronic Table of Contents (United Kingdom)

Optimization of nonviral gene delivery typically focuses on the design of particulate carriers that are endowed with desirable membrane targeting, internalization, and endosomal escape properties. Topographical control of cell transfectability, however, remains a largely unexplored parameter. Emerging literature has highlighted the influence of cell-topography interactions on modulation of many cell phenotypes, including protein expression and cytoskeletal behaviors implicated in endocytosis. Using high-throughput screening of primary human dermal fibroblasts cultured on a combinatorial library of microscale topographies, we have demonstrated an improvement in nonviral transfection efficiency for cells cultured on dense micropit patterns compared to smooth substrates, as verified with flow...

2011-01-01

434

Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers  

British Library Electronic Table of Contents (United Kingdom)

Occurrence of genetic variants during micropropagation is occasionally encountered when the cultures are maintained in vitro for long period. Therefore, the micropropagated multiple shoots of Vanilla planifolia Andrews developed from axillary bud explants established 10 years ago were used to determine somaclonal variation using random amplified polymorphic DNA (RAPD) and intersimple sequence repeats markers (ISSR). One thousand micro-plants were established in soil of which 95 plantlets (consisting of four phenotypes) along with the mother plant were subjected to genetic analyses using RAPD and ISSR markers. Out of the 45 RAPD and 20 ISSR primers screened, 30 RAPD and 7 ISSR primers showed 317 clear, distinct and reproducible band classes resulting in a total of 30 115 bands. However, no ...

2007-01-01

435

Extracellular matrix of plant callus tissue visualized by ESEM and SEM  

British Library Electronic Table of Contents (United Kingdom)

Actinidia deliciosa endosperm-derived callus culture is stable over a long period of culture. This system was used to investigate the ultrastructure of extracellular matrix occurring in morphogenic tissue. Specimens were prepared by different biological techniques (chemical fixation, liquid nitrogen fixation, glycerol substitution, critical-point drying, lyophilization) and observed by scanning electron microscopy (SEM). Fresh and wet samples were analyzed with the use of environmental scanning electron microscopy (ESEM). Extracellular matrix was observed on the surface of cell clusters as a membranous layer or reticulated network, shrunken or wrinkled, depending on the procedure. Generally, shrunken membranous layers with a globular appearance and fibrils were noted after critical-point d...

2010-01-01

436

Establishment of functional primary cultures of heart cells from the clam Ruditapes decussatus  

British Library Electronic Table of Contents (United Kingdom)

Heart cells from the clam Ruditapes decussatus were routinely cultured with a high level of reproducibility in sea water based medium. Three cell types attached to the plastic after 2?days and could be maintained in vitro for at least 1?month: epithelial-like cells, round cells and fibroblastic cells. Fibroblastic cells were identified as functional cardiomyocytes due to their spontaneous beating, their ultrastructural characteristics and their reactivity with antibodies against sarcomeric ?-actinin, sarcomeric tropomyosin, myosin and troponin T-C. Patch clamp measurements allowed the identification of ionic currents characteristic of cardiomyocytes: a delayed potassium current (I K?slow) strongly suppressed (95%) by tetraethylammonium (1?mM), a fast inactivating potassium current (I K?fas...

2011-01-01

437

Endurance of cultured pearls irradiated with gamma-rays  

International Nuclear Information System (INIS)

Pearls change their color to grey by #gamma#-ray irradiation. Grey densities were determined from darkness of pearl nuclei and thickness of the pearl layers. The densities are independent of both diameters of nuclei and pearls. The fading rate increases with increasing storage temperature. The rate of fading for irradiated pearls is lower than that for natural blue pearls. Comparison of photomicrographs (x 12) for pearls irradiated and then stored at room temperature for about 6 years revealed that their surfaces are not substantially different from the surfaces of unirradiated cultured pearls, indicating that #gamma# irradiation hardly gives rise to deterioration. #gamma#-Ray irradiation is a technique for coloration of pearls. Irradiated blue pearls seem to have resistivity to fading and to deterioration of the surface, if pearls have been irradiated properly. (author).

438

Effects of cultivar and culture conditions on g-aminobutyric acid accumulation in germinated fava beans (Vicia faba L.)  

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND: High levels of g-aminobutyric acid (GABA) accumulate in plant tissues under various stresses. GABA accumulation is also influenced by cultivar. This aim of this study was to select the most promising cultivar of fava bean for GABA accumulation and to optimise the culture conditions for GABA production in germinated fava beans by response surface methodology based on central composite design (CCD). RESULTS: GABA content and glutamate decarboxylase activity in germinated seeds of cultivar S2 were significantly higher than those in other cultivars (P < 0.05). A significant negative correlation (r = -0.765, P < 0.05) between germination percentage and 1000-kernel weight was observed. There was a linear relationship between GABA content and sprout length (R2 = 0.816). The regression...

2010-01-01

439

Do spatial effects appear at low dilution rate in chemostat?  

British Library Electronic Table of Contents (United Kingdom)

The chemostat theory on two species competition has shown that the dilution rate where transition of dominance occurs - transition-dilution rate - is independent of limiting-nutrient concentration. However, we obtained the experimental data indicating that the transition-dilution rate changed with variations in limiting-ammonium concentrations, using the chemostat mixed-culture of the cyanobacterium Microcystis novacekii and the green alga Scenedesmus quadricauda. The transition-dilution rate was dependent on the concentration of limiting ammonium in the influx culture medium. We tried to simulate the experimental results. Though the dilution rate has been considered independent of nutrient concentration, we introduce the effective dilution rate that depends on nutrient concentration (ammo...

2009-01-01

440

Comparative Evaluation of Different Cell Lysis and Extraction Methods for Studying Benzo(a)pyrene Metabolism in HT-29 Colon Cancer Cell Cultures  

British Library Electronic Table of Contents (United Kingdom)

Abstract Lysis and extraction of cells are essential sample processing steps for investigations pertaining to metabolism of xenobiotics in cell culture studies. Of particular importance to these procedures are maintaining high lysis efficiency and analyte integrity as they influence the qualitative and quantitative distribution of drug and toxicant metabolites in the intra- and extracellular milieus. In this study we have compared the efficiency of different procedures viz. homogenization, sonication, bead beating, and molecular grinding resin treatment for disruption of HT-29 colon cells exposed to benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) compound and a suspected colon carcinogen. Also, we have evaluated the efficiency of various procedures for extracting BaP parent c...

2011-01-01

441

Approaching the ?Resistant:? Exploring East Asian International Students? Perceptions of Therapy and Help-Seeking Behavior Before and After They Arrived in the United States  

British Library Electronic Table of Contents (United Kingdom)

In this study we investigated cultural, familial, and individual beliefs that influenced international students? perceptions of therapy and help-seeking behaviors. Included were changes in perception or behavior before and after the students came to the US, and factors that influenced help-seeking behaviors. Six international students from East Asian countries where English is a foreign language, including Taiwan, Mainland China, Japan, and South Korea, were interviewed. We used semi-structured interviews and participant observations for collecting data. The findings indicated changes after students came to the US. Further discussions focus on encouraging and discouraging factors relative to seeing therapists, and culturally sensitive interventions for family therapy clinicians.

2011-01-01

442

An in Vitro Assessment of Interaction Between Grape Phylloxera and Indole Acetic Acid Treated Grape Plants Daktulosphaira Vitifolia (FITCH)  

International Nuclear Information System (INIS)

the Life table of local strain of grape phylloxera was determined to evaluate the relationship between indole acetic acid (IAA) and phylloxera on our local variety Helwani. The study was carried out by applying in vitro dual culture system. The results showed that there was a great variation in mean developmental time, female longevity, number of laid eggs and egg distribution between all IAA concentrations and plant ages. Based on the tested biological parameters of phylloxera, (Helwani) would be unsuitable host for such destructive insect as it became older and when 2mg/1 of IAA was applied to in vitro culture media. (author)

443

Allelopathic interactions between Prorocentrum micans and Skeletonema costatum or Karenia mikimotoi in laboratory cultures  

British Library Electronic Table of Contents (United Kingdom)

Algal allelopathy is an ecological/physiological phenomenon that has focused attention on the interactions among algae and the production of algal toxins. We investigated the allelopathic interactions between the dinoflagellate genus Prorocentrum micans and diatom genus Skeletonema costatum and between P. micans and dinoflagellate genus Karenia mikimotoi using bi-algal cultures. Because the effects were species-specific and size-dependent, we evaluated the effect of different initial densities. At low densities of P. micans and high densities of S. costatum inoculated into the same medium, the growth of P. micans was weakly restrained, whereas the growth of S. costatum was significantly suppressed. S. costatum and K. mikimotoi were strongly inhibited by P. micans, in both the bi-algal cult...

2011-01-01

444

Alcohol fermentation in olive oil extraction effluents  

Energy Technology Data Exchange (ETDEWEB)

Eight culture-collection yeast strains of various species and five newly isolated stains were tested for both growth in olive oil extraction effluents and fermentation of the sugars in the same media. The culture-collection yeast strains did not grow in an effluent containing 2.86% sugar (w/v), 8 g/litre phenolic substances, 4.58 g/litre titratable acidity and pH 4.96, whereas the newly isolated strains of Torulopsis sp. MK-1, Saccharomyces norbensis MC-1, S. oleaceus MC-2 and S. oleaginosus grew well and fermented the sugars. In the medium mentioned above, they produced alcohol in amounts of 1.63 to 1.38%, respectively. None of the yeasts grew in an olive oil extraction effluent vacuum-concentrated to over 13-14% of dry matter. The strain of T. sp. MK-1 showed a higher stability.

1989-01-01

445

5,8,11,14-Eicosatetraynoic acid-induced destruction of mitochondria in human prostate cells (PC-3).  

Energy Technology Data Exchange (ETDEWEB)

Culturing human prostate PC-3 cells for 4, 24, or 72 h in the presence of 5,8,11,14-eicosatetraynoic acid (ETYA), an inhibitor of arachidonic acid metabolism and cholesterol biosynthesis, markedly altered the morphology and reduced the number of mitochondria in the treated cells. Using quantitative electron microscopic morphometry, we documented changes in the number, form, area, matrix density, and integrity of the cristae and limiting membranes of mitochondria in cells cultured with ETYA. The inhibition of cholesterol synthesis or the substitution of ETYA for polyunsaturated fatty acids in the inner membrane may participate in the disruption of the mitochondria, which resembles the morphologic sequelae of oxidative stress. If sufficiently extensive, these changes could contribute to the inhibition of cellular proliferation by ETYA.

1992-01-01

446

Use of a new time of flight aerosol mass spectrometer for ground and airborne measurements of biogenically produced organic aerosols  

Environmental Research Database

DescriptionChamber studies have shown that biogenic organic compounds, including isoprene, are capable of producing secondary organic aerosol in significant quantities. This is surprising as isoprene and its photo-oxidation products have relatively high vapour pressures. However, it is very important as isoprene has the largest global emissions flux of any biogenic hydrocarbon, most of which is in the tropics. Despite this, secondary organic aerosol has been poorly studied in tropical regions with only [continued...

447

The Mission, Organization, and Functions of US Army Rear ...  

Science.gov (United States)

... counseling, some training, and assure the general health and welfare of the command. ... publications, promotions, OERs/NCOER's (Officer Efficiency ...

1992-06-07

448

Taming the Frontier: A Myth of Impossibility  

Science.gov (United States)

... 5. FUNDING NUMBERS ... SEATO SOUTHEAST ASIA TREATY ORGANIZATION TTP TEHRIK-I-TALIBAN PAKISTAN Page 13. xii ...

2010-03-01

449

Synthesizing Information for Interagency Decision Makers ...  

Science.gov (United States)

... Principal Author's Organization and address: BAE Systems, AIT 6 New England Executive Park Burlington, Massachusetts 01803 ...

2007-06-01

452

Quality assurance requirements in the engineer/constructor organization  

International Nuclear Information System (INIS)

(Jun 1975). United States Bernsen, SA Bechtel Corp., San Francisco New

1975-06-08

453

Plastics with a bright future; Kunststoffe mit leuchtender Zukunft  

Energy Technology Data Exchange (ETDEWEB)

Flexible solar cells or luminous foils have become reality with organic electronic systems.

2010-07-01

454

Organizational demands on the man-organization interface in nuclear power plant operation  

International Nuclear Information System (INIS)

The partial project SR 2039/4 investigates organizational demands and factors of the man-organization interface. Thee study is divided into the sections inventory of rules and guidelines; evaluation of organizational structures and modes of operation, and working out of demand characteristics and evaluation criteria. The objective consists in representing, analyzing and evaluating the efficiency structure of sequence organization, and deriving practice-relevant concept proposals in order to optimize the man-organization interface. In this regard, the working out of control mechanisms for the evaluation of organizational sequences is of considerable importance as a kind of working aid. (orig./DG).

1994-04-01

455

National Ignition Facility Management Descriptions Revision 4  

Energy Technology Data Exchange (ETDEWEB)

The purpose of this document is to describe the NIF Project Organization and the roles and responsibilities of the managers charged with executing the Project.

2000-08-01

456

Mass fragmentographic determination of polymethylnaphthalene and polymethylphenanthrene in a crude oil and in marine organisms  

Energy Technology Data Exchange (ETDEWEB)

The investigation of the petroleum contamination in marine organisms requires the trace level quantitative determination of indicative substances to be identified by a fingerprint of petroleum. Polymethylnaphthalene (PMN) and polymethylphenanthrene (PMP) were chosen because of their simple separation from a crude oil by means of an activated charcoal chromatography, their relatively high existence in aromatic compounds, and their persistence relative to other petroleum components in the tissue of oil contaminated marine organisms. The purpose of this paper is to present an activated charcoal chromatographic separation procedure of PMN and PMP in a crude oil and a trace level quantitative analytical method of them in marine organisms by mass fragmentography (MF).

1980-09-01

457

Is the US Army a Learning Organization?  

Science.gov (United States)

... outside observers, and updated counseling requirements for the Army's OER, non-commissioned officer efficiency report (NCOER), and civilian ...

2007-03-08

458

General Toxicity/Reproductive Toxicity Screen of Modular ...  

Science.gov (United States)

... BODY WEIGHT, FEMALES, SEX, ORGANS(ANATOMY), NITROGUANIDINE, NITROGLYCERIN, COMBUSTIBLE CARTRIDGE CASES, FERTILITY. ...

1996-01-01

459

Elemental and stable isotopic approaches for studying the organic and inorganic carbon components in natural samples  

Energy Technology Data Exchange (ETDEWEB)

The carbon cycle is an important part of major biogeochemical cycles. Many techniques may be used to characterize carbon amounts and sources in the environment. Here we first review the most popular techniques for the determination of organic and inorganic carbon concentrations. Decarbonatation techniques are also reviewed in details since it is often an important part of organic carbon analysis. The second part of this paper addresses the use of carbon stable isotopes to characterize organic carbon sources and processes in the environment. An overview of general stable isotopes background and terminology is given as well as the most popular analytical techniques.

2009-01-01

462

Composition of insoluble kerogen-like organic polymer in the carbonate rocks of Orenburg gas-condensate deposit  

British Library Electronic Table of Contents (United Kingdom)

The concentrates of scattered organic matter???kerogen-like polymer, which is insoluble in acids and organic solvents???were isolated from rock samples taken at different depths from various stratigraphic units at the productive deposits of the Orenburg oil-gas condensate field using chemical methods. The chemical composition of the kerogen-like polymer and the concentrations of trace elements in it were studied. The regularities of evolutionary changes in the composition of the organic matter and the concentrations of trace elements in it in the order of the kerogen-like polymer and the following its consecutive derivatives were established: asphaltenes, ethanol-benzene tars, benzene tars, oils, and paraffins

2011-01-01

463

Accelerated aging tests for radiation degradation of organic materials  

International Nuclear Information System (INIS)

(Jun 1984). United States Clough, RL Gillen, KT Sandia Nat'l Laboratories

1984-06-03

464

/linkages/journal/ Volume 6 Number 8  

Wastenet

The Commission also agreed on new guidelines for organic livestock production, supporting natural breeding methods and

465

Sustainable pest control - comparing tritrophic interactions in organic and conventional production systems  

Environmental Research Database

DescriptionThis cross-disciplinary project will combine chemical, ecological and modelling techniques to determine whether cabbages grown under an organic regime differ in terms of pest dynamics and plant chemistry. There is increasing pressure to de-intensify agricultural practice and organic approaches are becoming more popular and widely adopted. However, there are very few, if any, detailed scientific investigations into the claims made about improved pest control, reduced environmental impact and be [continued...

2009-01-31

466

Pore size distribution in ZrO_2-Al_2O_3 composite particles prepared from Zr-Al metallo-organic compounds  

International Nuclear Information System (INIS)

Zr-Al metallo-organic compounds were thermally decomposed to prepare ZrO_2-Al_2O_3 composite powders. The specific surface area was dependent on the organofunctional groups of the starting organic compounds. The pore size distribution was dependent on the heating rate when the heating temperature was the same. 4 refs.; 4 figs.

1989-01-01

467

Modalidade de morte em mamiferos expostos a irradiacao de corpo inteiro (sindrome aguda da radiacao). (Modes of death in mammals exposed to whole body radiation (acute radiation syndromes)).  

Science.gov (United States)

When an animal is exposed to a sufficient amount of radiation, there will be changes in many organs of the body, and as a result of either the effects in one particular organ or the interaction of effects in several organs, the animal as a whole will show...

1990-01-01

468

Method for the elimination of simple organic compounds by filtration on activated-charcoal particles  

Energy Technology Data Exchange (ETDEWEB)

The use of filters of activated-charcoal particles in drinking-water purification plants assists in the elimination of organic micropollutant substances. The authors report on a study which sought to investigate the mechanism of organic matter; they describe the experimental programme, which used different size ranges of mineral-based activated charcoal, present the experimental findings and discuss the results. 16 references.

1984-12-01

469

Investigation of morphology and chemical composition of self-organized semiconductor quantum dots and wires by X-ray scattering  

International Nuclear Information System (INIS)

X-ray scattering methods suitable for the investigation of the morphology and chemical composition of self-organized quantum dots and quantum wires are reviewed. Their application is demonstrated in experimental examples showing that a combination of small angle X-ray scattering with high-resolution X-ray diffraction can reveal both the shape and the chemical composition of the self-organized objects. (author)

2001-09-23

470

Impact of repeated two-phase olive mill waste application on phosphorus fractionation in a degraded olive grove soil  

Energy Technology Data Exchange (ETDEWEB)

Loss of organic matter is one of the main forms of soil degradation in Mediterranean agricultural soils, and external sources of organic matter are required to improve soil properties. the two-phase centrifugation system in the olive-oil extraction industry produces a large amount of olive mill waste sludge (TPOMW) which can be used to add organic C to degraded soils. (Author)

2009-07-01

471

Evaluation of indoor and outdoor climate on sites polluted with volatile organic chemicals  

International Nuclear Information System (INIS)

Papers presented at a meeting on indoor and outdoor climates on sites polluted with volatile organic chemicals. The papers deal with the subject of evaporation of organic chemicals on the polluted sites in relation to the influence on indoor and outdoor climates. Themes dealt with are diffusion through soils and transport of pollutants from the soil into buildings. (AB).

1993-11-04

472

Determination of Fe and Zn in agricultural plants with special cultivation by radionuclide x-ray fluorescence analysis  

International Nuclear Information System (INIS)

Fe and Zn were determined in various parts of maize (Zea mays) in dependence on quantity of organic substrate EKOFERT as organic fertilizer in soil, using radionuclide X-ray fluorescence analysis. The increase of quantity of organic substrate EKOFERT in soil causes a decrease of heavy metal concentrations in certain parts of the plant. (author). 4 refs., 1 tab.

1996-01-01

473

Coastal metabolism and the oceanic organic carbon balance  

Energy Technology Data Exchange (ETDEWEB)

The metabolism of organic matter in the coastal regions of the ocean may significantly affect the oceanic carbon budget. This paper describes the high percentage (30%) of oceanic oxidation that occurs in coastal areas and the impact of this metabolism on the carbon cycle and climate of earth. Organic metabolism in the ocean appears to be a source of carbon dioxide release into the atmosphere. Oxidation in the coastal zone is of special interest, as it is likely influenced by anthropogenic activity. Recommendations for future research on this topic are proposed. 129 refs., 2 figs., 6 tabs.

1993-02-01

474

BBSRC CASE studentship: The impact of nutrition on the gluten composition and processing quality of wheat  

Environmental Research Database

DescriptionThe objective of this project is to relate the processing quality of wheat to the composition of the developing and mature grain using material grown under a range of nutrient regimes including long term organic and fertilised systems from the Rothamsted Broadbalk experiment, variety trials and organically grown wheat. Preliminary studies have indicated substantial differences in the transcriptome profiles of wheat grown with artificial fertiliser and with organic fertiliser on Broadbalk and tru [continued...

2008-01-31

475

Advances in Library Administration and Organization  

CERN Document Server

The Advances in Library Administration and Organization Series provides a body of research literature that contributes to the base of organizational theory upon which library administrators rely. Its mix of contributions to the literature of library administration and organization is both diverse and eclectic. This volume of the series covers a variety of topics relating to the management of academic, public and school libraries

2010-01-01

476

The solubilities of significant organic compounds in HLW tank supernate solutions  

International Nuclear Information System (INIS)

Large quantities of organic chemicals used in reprocessing spent nuclear-fuels at the Hanford Site have accumulated in underground high-level radioactive waste tanks. The organic content of these tanks must he known so that the potential for hazardous reactions between organic components and sodium nitrate/nitrite salts in the waste can he evaluated. The solubilities of organic compounds described in this report will help determine if they are present in the solid phases (salt cake and sludges) as well as the liquid phase (interstitial liquor/supernate) in the tanks. The solubilities of five significant sodium salts of carboxylic acids and aminocarboxylic acids [sodium oxalate, formate, citrate, nitrilotriacetate (NTA) and ethylendiaminetetraacetate (EDTA)] were measured in a simulated supernate solution at 25 degrees C, 30 degrees C, 40 degrees C, and 50 degrees C.

1994-08-21

477

Role of natural organic matter (NOM), colloidal particles, and solution chemistry on ultrafiltration performance  

British Library Electronic Table of Contents (United Kingdom)

Mechanistic studies on a charged ultrafiltration (UF) membrane fouled with natural organic matter (NOM) and colloidal particles are systematically investigated to understand the relative role of each NOM fraction and the presence of colloidal particulate to membrane fouling. Humic acid (HA), dextran, and kaolin were employed as surrogate model foulants representing the organic hydrophobic acid NOM, hydrophilic neutral NOM, and inorganic colloidal materials, respectively. The results obtained showed that the organic NOM of hydrophilic surrogate (dextran) plays a primary role in promoting membrane fouling, followed by hydrophobic acids and inorganic kaolin, but to a lesser extent than organic NOM compounds. Significant differences in the extent of fouling between dextran and HA have been obs...

2011-01-01

478

Recovery of soil organic matter, organic matter turnover and nitrogen cycling in a post-mining forest rehabilitation chronosequence  

British Library Electronic Table of Contents (United Kingdom)

Recovery of soil organic matter, organic matter turnover and mineral nutrient cycling is critical to the success of rehabilitation schemes following major ecosystem disturbance. We investigated successional changes in soil nutrient contents, microbial biomass and activity, C utilisation efficiency and N cycling dynamics in a chronosequence of seven ages (between 0 and 26 years old) of jarrah (Eucalyptus marginata) forest rehabilitation that had been previously mined for bauxite. Recovery was assessed by comparison of rehabilitation soils to non-mined jarrah forest references sites. Mining operations resulted in significant losses of soil total C and N, microbial biomass C and microbial quotients. Organic matter quantity recovered within the rehabilitation chronosequence soils to a level co...

2008-01-01

479

Mechanisms of Self-Organization and Finite Size Effects in a Minimal Agent Based Model  

CERN Document Server

We present a detailed analysis of the self-organization phenomenon in which the stylized facts originate from finite size effects with respect to the number of agents considered and disappear in the limit of an infinite population. By introducing the possibility that agents can enter or leave the market depending on the behavior of the price, it is possible to show that the system self-organizes in a regime with a finite number of agents which corresponds to the stylized facts. The mechanism to enter or leave the market is based on the idea that a too stable market is unappealing for traders while the presence of price movements attracts agents to enter and speculate on the market. We show that this mechanism is also compatible with the idea that agents are scared by a noisy and risky market at shorter time scales. We also show that the mechanism for self-organization is robust with respect to variations of the exit/entry ...

2008-01-01

480

Extraction of hafnium by 1-phenyl-3-methyl-4-benzoyl-5-pyralozone from aqueous-alcoholic solutions  

International Nuclear Information System (INIS)

Extraction of hafnium by 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (HL) in benzene, toluene, chloroform and tetrachloromethane from aqueous-alcoholic solutions of the formal acidity of 2M-HClO_4 was studied. Methyl, ethyl, n- and isopropyl, tert-butyl and allyl alcohol as well as ethylene glycol monomethyl ether and propylene glycol were used as organic components of the mixed aqueous-organic phase. Their presence in some cases resulted in a synergic increase in the distribution ratio of hafnium. The increase is interpreted using the results of a slope analysis and measurements of the alcohol distribution and the relative permittivity of the organic phase. It is suggested that HfL_4 molecules were solvated by alcohol molecules in the organic phase. At high alcohol concentration synergism changed into antagonism. This was caused by changes in the distribution of HL and its interaction with the alcohol in ...

481

Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant1[W][OA]  

UK PubMed Central (United Kingdom)

We characterized the absorption and short-term translocation of cadmium (Cd) in rice (Oryza sativa ‘Nipponbare’) quantitatively using serial images observed with a positron-emitting...Full Text Available

2010-04-01

482

The association of asymptomatic bacterial vaginosis with upper genital tract infection.  

Science.gov (United States)

Objective: To determine whether asymptomatic bacterial vaginosis (BV) is associated with upper genital tract inflammation or bacterial colonization.Methods: Fifty nonpregnant women with intact uteri who planned to undergo gynecologic surgery and had no recent symptoms of vaginal infection were enrolled. We obtained a vaginal swab for Gram stain, endocervical swab for chlamydia and gonorrhea DNA probe testing, and Pipelle endometrial biopsy for aerobic and anaerobic cultures and histology. We correlated surgical findings and histology of available surgical specimens with the microbiologic results. The diagnosis of BV was made according to Speigel's criteria. Bacteria isolated from the uterus were classified as high virulence versus low virulence. Contingency tables were analyzed using the chi-square or Fisher Exact tests.Results: Twenty-one of 50 patients had BV on Gram stain, 3 had intermediate BV, and 3 had unreadable slides. Eleven patients had histologic ...

1998-07-01

483

Structure of newly synthesized (/sup 35/S)-proteoglycans and (/sup 35/S)-proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis  

Energy Technology Data Exchange (ETDEWEB)

The structure of newly synthesized proteoglycans from explant cultures of cartilage from joints subjected to transection of the anterior cruciate ligament (osteoarthritic) and from normal (non- or sham-operated) joints was examined. The structure of the products of proteoglycan turnover was also examined using explants of normal and osteoarthritic cartilage maintained in culture for a 48 h chase period. The findings were as follows: Newly synthesized (/sup 35/S)-proteoglycans extracted from cartilage explants from osteoarthritic joints whether examined 3 weeks, 3 months, or 6 months after surgery were larger than those from corresponding normal cartilage. This can be explained by the synthesis in osteoarthritic cartilage of abnormally long chondroitin sulfate chains on newly synthesised proteoglycans. The extracts also contained a newly formed small proteoglycan species that was unable to interact with hyaluronic acid. The proportion of this ...

1985-01-01

484

Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line.  

UK PubMed Central (United Kingdom)

The ERA strain of rabies virus was propagated in a baby hamster kidney cell line (BHK-21/C13). The viral titer was 10(1.8) tissue culture infective doses (TCID) higher than that of commercial ERA vaccine....Full Text Available

1989-10-01

485

Relationship between Indole-3-Acetic Acid Levels in Apple (Malus pumila Mill) Rootstocks Cultured in Vitro and Adventitious Root Formation in the Presence of Indole-3-Butyric Acid 1  

UK PubMed Central (United Kingdom)

In vitro rooting response and indole-3-acetic acid (IAA) levels were examined in two genetically related dwarfing apple (Malus pumila Mill) rootstocks. M.26 and M.9...Full Text Available

1989-02-01

486

Nature of plant stimulators in the production of Acetobacter xylinum ({open_quotes}Tea fungas{close_quotes}) biofilm used in skin therapy  

Energy Technology Data Exchange (ETDEWEB)

Caffeine and related xanthines were identified as potent stimulators for the bacterial cellulose production in A. xylinum. These compounds are present in several plants whose infusions are useful as culture-medium supplements for this acetobacterium. The proposed target for these native purine-like inhibitory substances is the novel diguanyl nucleotide phosphodiesterase(s) that participates in the bacterial cellulogenic complex.

1991-12-31

487

NAME=\\  

Wastenet

... Based in the Philippines, it has a broad remit, covering all philosophical topics, but with a particular focus on analytic ...net/ Cultura is a Romanian-based international journal devoted to philosophy of culture and axiology (the study of value). It aims ...edu/ Aporia is an online undergraduate philosophy journal based at Brigham Young University in Utah, USA. All articles are written by undergraduates,...edu/pcs/basic.html Theoretical and Conceptual Advances in the Cognitive Neuroscience of Self Representation: Representations of the Minimal Self in Self-Narrative is ...

488

NAME=\\  

Wastenet

... This section provides free access to resources relating to politics, political participation, political culture and democracy in Dominican Republic. Complete survey data files may be requested. Street museum An interesting new app from the Museum of London. It enables you to view old images of London (taken from the Museums vast collection- dating from the Fire of London in 1666 to the 1960s). ...

489

Mutation breeding in kalmia juvenile trees derived from shoot tip cultare and kobus seedlings  

International Nuclear Information System (INIS)

Juvenile trees of kalmia derived from shoot tip culture, and seedlings of kobus were irradiated with #gamma#-rays. In kalmia, two mutation lines were obtained which had narrowlong leaves. In kobus two mutation lines were obtained. One mutation line has flowers with 7 to 13 petals. Another mutation line has yellow-green variegated leaves. (author).

490

Microfabricated polyester conical microwells for cell culture applications.  

Science.gov (United States)

Over the past few years there has been a great deal of interest in reducing experimental systems to a lab-on-a-chip scale. There has been particular interest in conducting high-throughput screening studies using microscale devices, for example in stem cell research. Microwells have emerged as the structure of choice for such tests. Most manufacturing approaches for microwell fabrication are based on photolithography, soft lithography, and etching. However, some of these approaches require extensive equipment, lengthy fabrication process, and modifications to the existing microwell patterns are costly. Here we show a convenient, fast, and low-cost method for fabricating microwells for cell culture applications by laser ablation of a polyester film coated with silicone glue. Microwell diameter was controlled by adjusting the laser power and speed, and the well depth by stacking several layers of film. By using this setup, a device containing hundreds of microwells ...

2011-05-26

491

Lessons learned from accidents investigations  

International Nuclear Information System (INIS)

Accidents from three main practices: medical applications, industrial radiography and industrial irradiators are used to illustrate some common causes of accidents and the main lessons to be learned. A brief description of some of these accidents is given. Lessons learned from the described accidents are approached by subjects covering: safety culture, quality assurance, human factors, good engineering practice, defence in depth, security of sources, safety assessment and monitoring and verification compliance. (author)

1997-10-26

492

IGF 2 expression in 3T3 adipocytes in response to serum from hypophysectomized or diabetic swine  

Energy Technology Data Exchange (ETDEWEB)

Expression of IGF-2 and changes in its expression in response to systemic endocrine alterations have not been demonstrated for adipocytes. Adipocytes were induced to develop within cultures of 3T3-L1 cells using a medium containing 0.5mM isobutylmethylxanthine, 1uM insulin and 100ng hydrocortisone/ml for 48 hours of exposure. Cultures containing developing adipocytes were incubated with 10% pig serum and 1 uM insulin for several days. The resultant adipocyte cultures were then treated with either 10% pig serum, diabetic pig serum or hypophysectomized pig serum in DMEM for 48 hours. Adipocytes within the cultures were separated from undifferentiated cells using percoll density gradient centrifugation. Total RNA was isolated from adipocytes and dot blotted. Blots were probed with a {sup 32}P-cDNA probe for rat IGF-2. IGF-2 was expressed by the adipocytes and the pattern of expression showed specific ...

1990-02-26

493

Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS), p38MAPK, and Akt  

UK PubMed Central (United Kingdom)

Chinese hamster lung fibroblasts V79 cells were treated with heat stress for 4 weeks with short duration (15 min) heat shock every alternate day in culture. It was observed that Hsp...Full Text Available

2009-11-01

494

Cutaneous manifestations in brucellosis.  

Science.gov (United States)

Brucellosis is a common worldwide zoonotic disease. Cutaneous manifestations are not specific and affect 1-14% of patients with brucellosis. Here, we describe 49-year-old female with fever and a diffuse maculopapular rash due to Brucella melitensis infection. Histopathology of skin biopsy revealed leukocytoclastic vasculitis; positive blood cultures for B. melitensis established the diagnosis of brucellosis. We provide a review of the relevant literature. PMID:21772606

2011-05-01

495

Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane chorion cells undergoing apoptosis after influenza virus infection.  

Science.gov (United States)

Influenza virus infection during pregnancy has been implicated as one of cause of premature delivery, abortion and stillbirth. We have reported that cultured human fetal membrane chorion cells undergoing apoptosis by influenza virus infection secrete unidentified heat-stable monocyte differentiation-inducing (MDI) factors. In this study, cellular, biological and immunochemical characteristics of MDI factors were investigated using human monocytic leukemia THP-1 cells by nitroblue tetrazolium reduction and cell adhesion assays. The treatment of THP-1 cells with culture supernatants from the influenza virus-infected chorion cells induced the nitroblue tetrazolium reduction ability, which was inhibited by the addition of superoxide dismutase and diphenyleneiodonium chloride, an inhibitor for reduced nicotinamide adenine dinucleotide phosphate oxidase. The phenomenon was also observed in human peripheral blood monocytes and histiocytic leukemia ...

2006-06-06

496

Astrocytes derived from trisomic human embryonic stem cells express markers of astrocytic cancer cells and premalignant stem-like progenitors  

UK PubMed Central (United Kingdom)

BackgroundTrisomic variants of human embryonic stem cells (hESCs) arise spontaneously in culture. Although trisomic hESCs share many properties with diploid hESCs, they also exhibit...Full Text Available

497

Action of Inhibitors of Ammonia Assimilation on Amino Acid Metabolism in Hordeum vulgare L. (cv Golden Promise) 1  

UK PubMed Central (United Kingdom)

Barley (Hordeum vulgare L. cv Golden Promise) plants were grown in a continuous culture system in which the root and shoot ammonia and amino acid levels were constant over a 6-hour...Full Text Available

1983-03-01

498

A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM  

UK PubMed Central (United Kingdom)

BackgroundThermostable bacterial lipases occupy a place of prominence among biocatalysts owing to their novel, multifold applications and resistance to high temperature and other...Full Text Available

499

4.5S RNA is encoded by hundreds of tandemly linked genes, has a short half-life, and is hydrogen bonded in vivo to poly(A)-terminated RNAs in the cytoplasm of cultured mouse cells.  

UK PubMed Central (United Kingdom)

4.5S RNA is a group of RNAs 90 to 94 nucleotides long (length polymorphism due to a varying number of UMP residues at the 3' end) that form hydrogen bonds with poly(A)-terminated RNAs isolated from...Full Text Available

1986-05-01

500

31P NMR Observations on the Effect of the External pH on the Intracellular pH Values in Plant Cell Suspension Cultures 1  

UK PubMed Central (United Kingdom)

31P nuclear magnetic resonance (NMR) spectroscopy was used to monitor the response of oil palm (Elaeis guineensis) and carrot (Daucus carota) cell suspensions...Full Text Available

1990-06-01