hairy root culture: Topics by WorldWideScience.org

Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture

During the symbiotic interaction between Medicago truncatula and the arbuscular mycorrhizal (AM) fungus Glomus intraradices, an endogenous increase in jasmonic acid...Full Text Available

2005-11-01

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Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture

Micropropagation methods are described for the production of clonal lines from Eucalyptus marginata (jarrah) seedlings. Nodal explants from mature trees can also yield shoot cultures, but a high frequency of contamination occurs among such explants. Uncontaminated callus cultures can be produced from mature trees by culturing stamen filaments and shoots can subsequently be regenerated from this callus. The rooting percentage of shoot cultures from either nodes or stamen callus of mature trees is low compared with that from seedling explants. Considerable variation was observed between trees in the ability of stamen callus to regenerate shoots and in the frequency of rooting. (Refs. 27)

1982-01-01

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An efficient in vitro plantlet regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through shoot tip culture

Methods are described for the production of callus and subsequent shoot and root induction and multiplication using stamen filaments, nodes of young stems of mature trees or cotyledon petioles. A high frequency of contamination occurred using nodal sections from mature trees, whereas up to 100% sterility was obtained with cotyledons and stamens. The rooting % of shoot cultures from nodes or stamen callus was low compared with that of shoots from seedling callus. Considerable variation was observed between clones from different trees in their ability to produce shoots and roots. 27 references

1982-01-01

4

British Library Electronic Table of Contents (United Kingdom)

An efficient micropropagation protocol was established for Cryptocoryne wendtii and Cryptocoryne becketti using shoot tips explants. Multiple shoots were induced from shoot tip explants of both species cultured on agar-gelled as well as liquid MS medium supplemented with 0.5?mg/L BA and 0.2?mg/L IBA (proliferation medium). The multiple shoots of both the species formed on agar-gelled as well as liquid medium were vigorously growing with well-developed roots and leaves after 4?weeks of culture. Highest number of multiple shoots was obtained from shoot tip explants of both the species cultured in liquid proliferation medium after 4?weeks of culture. The shoot tip explants of C. wendtii and C. becketti, that were cultured in liquid proliferation medium (2?weeks) followed by culturing on agar-...

2011-01-01

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Quantitation of Cellular Dynamics in Growing Arabidopsis Roots with Light Sheet Microscopy

Relationship between Indole-3-Acetic Acid Levels in Apple (Malus pumila Mill) Rootstocks Cultured in Vitro and Adventitious Root Formation in the Presence of Indole-3-Butyric Acid ¹

To understand dynamic developmental processes, living tissues must be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in indeterminately growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image at cellular resolution a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, track cellular nuclei and identify cell divisions. We ...

2011-01-01

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Tissue culture process for the clonal production of loblolly pine plantlets

In vitro rooting response and indole-3-acetic acid (IAA) levels were examined in two genetically related dwarfing apple (Malus pumila Mill) rootstocks. M.26 and M.9...Full Text Available

1989-02-01

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Contribution of heavy metals and As-loaded lupin root mineralization to the availability of the pollutants in multi-contaminated soils

A tissue culture process, organogenesis, is described which involves the stepwise production of a shoot apex, which grows to have leaves and stem and finally roots, culminating in the production of a complete plant. The production and subsequent development of numerous adventitious shoots, preferably from adult tree tissues offers the greatest potential for commercial cloned plantlet production. The process describes these steps for Pinus taeda, the loblolly pine. (Refs. 25).

1981-02-01

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Sequential Sampling Plans for the Hairy Chinch Bug (Hemiptera: Lygaeidae)

White lupin (Lupinus albus L.) is an annual crop that has been used for phytostabilization of acidified multi-contaminated soils. Once the culture cycle is over, after shoot harvesting, a progressive transference of contaminants from roots to soil may take place as decomposition of roots occurs. An incubation experiment with Cu, Zn, Cd, and As-loaded roots of white lupin and soils with different pH values and concentrations of these contaminants from the area affected by a mine spill at Aznalcollar (near Seville, Spain) was performed in order to assess the effect of the decomposition of the roots to the pH and (NH_4)_2SO_4-extractable levels of these pollutants in the soils. Pollutants loaded-roots were mineralized (56 d) at a ratio similar to animal manures (15.8-19.4% of total organic carbon) in soil. The estimated root inputs of ...

2008-03-01

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Site of lupanine and sparteine biosynthesis in intact plants and in vitro organ cultures

... was log (s2) = 1.183 + 1.692 log (x) (r2 = 0.96; df = 1, 43; F = 1067.40; P kc was calculated according to Ruesink (1980), using 15. ... ...

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Waterlogging-induced increase in sugar mobilization, fermentation, and related gene expression in the roots of mung bean (Vigna radiata)

["1"4C]Cadaverine was applied to leaves of Lupinus polyphyllus, L. albus, L. angustifolius, L. perennis, L. mutabilis, L. pubescens, and L. hartwegii and it was preferentially incorporated into lupanine. In Lupinus arboreus sparteine was the main labelled alkaloid, in L. hispanicus it was lupanine. A pulse chase experiment with L. angustifolius and L. arboreus showed that the incorporation of cadaverine into lupanine and sparteine was transient with a maximum between 8 and 20 h. Only leaflets and chlorophyllous petioles showed active alkaloid biosynthesis, whereas no incorporation of cadaverine into lupanine was observed in roots. Using in vitro organ cultures of Lupinus polyphyllus, L. succulentus, L. subcarnosus, Cytisus scoparius and Laburnum anagyroides the inactivity of roots was confirmed. Therefore, the green aerial parts are the major site of alkaloid biosynthesis in lupins and in other legumes. (orig.).

1987-01-01

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British Library Electronic Table of Contents (United Kingdom)

Summary The objective of this study was to examine the role of root carbohydrate levels and metabolism in the waterlogging tolerance of contrasting mung bean genotypes. An experiment was conducted with two cultivated mung bean (Vigna radiata) genotypes viz., T44 (tolerant) and Pusa Baisakhi (PB) (susceptible), and a wild Vigna species Vigna luteola under pot-culture to study the physiological and molecular mechanism of waterlogging tolerance. Waterlogging resulted in decrease in relative water content (RWC), membrane stability index (MSI) in root and leaf tissues, and chlorophyll (Chl) content in leaves, while the Chl a/b ratio increased. Waterlogging-induced decline in RWC, MSI, Chl and increase in Chl a/b ratio was greater in PB than V. luteola and T44. Waterlogging caused decline in tot...

2009-01-01

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Uptake and translocation of {sup 137}Cs by Houttuynia cordata (in water culture)

Technetium transfer from soil to plants

The water culture experiment of Houttuynia cordata of the medicinal plant was carried out, and basic study on {sup 137}Cs accumulation characteristic and internal circulation in H. cordata in the fruiting stage was investigated since the flowering season. H. cordata accumulated 80% of {sup 137}Cs absorbed unlike K to the root, rhizome, terminal bud of the underground part for the rhizome reproduction. {sup 137}Cs content to the young leaf, spike, involucre increased in the flowering season. Thereafter, {sup 137}Cs was recirculated to the developing organs in second generations such as the rhizome and bud. (author)

2001-08-01

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Action of Inhibitors of Ammonia Assimilation on Amino Acid Metabolism in Hordeum vulgare L. (cv Golden Promise) 1

Technetium transfer from soil to edible parts of various agricultural plants is studied with application of the "9"5"mTc radioactive tracer. The samples of agricultural plants were grown on andesol typical for Japan soil. The technetium transfer factor to edible parts of cultivated lettuce was higher as compared to non foliate cultures. Relative low transfer factor were observed for fruit and pod like plants. the transfer factors for root crops were of intermediate value

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Lecturas neobarrocas del Espejo de paciencia y reconceptualizaciones de la naci?n: Jos? Lezama Lima, Cintio Vitier y Severo Sarduy

Barley (Hordeum vulgare L. cv Golden Promise) plants were grown in a continuous culture system in which the root and shoot ammonia and amino acid levels were constant over a 6-hour...Full Text Available

1983-03-01

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British Library Electronic Table of Contents (United Kingdom)

This article examines the successive reinterpretations of one of Cuba?s foundational texts, namely the Espejo de paciencia (?Model of Patience??, 1608) by Silvestre de Balboa, as a baroque poem. The revalorization of the baroque by twentieth-century Cuban authors and critics has been explained as a consequence of the erasure of indigenous cultures and the subsequent imposition of a metropolitan culture. In this sense, the baroque is supposed to be paradigmatic for ?roots thinking?? about nation-building and culture. The readings of Balboa?s poem by neo-baroque writers such as Jos? Lezama Lima, Cintio Vitier and Severo Sarduy, however, put forward a transhistorical vision of the baroque and Cubanhood as an eternal and immutable phenomenon or, alternatively, a reading that privileges the tec...

2010-01-01

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Improvement of banana through biotechnology and mutation breeding

Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy

Protocols were standardized for in vitro propagation of several elite and diverse banana accessions using shoot tip explants. Tissue culture raised plants were field planted at multiple locations. Studies were undertaken for the induction of mutations using multiple shoot cultures of six selected cultivars, Shreemanti (AAA), Basrai (AAA), Lal Kela (AAA), Rasthali (AAB), Karibale Monthan (ABB) and a wild diploid (BB). These shoot cultures were irradiated at different doses of gamma rays (0-100 Gy) and subcultured thrice (up to M_1V_3) to separate shimeras, followed by induction of rooting (M_1V_4). In general, the rate of multiplication had a negative association with the dose of gamma rays. Enhanced multiplication of shoots was noticed at lower doses. The proliferation of shoots was arrested beyond 50 Gy and a dose of 70 Gy was completely lethal for all the genotypes studied. The ...

1998-10-01

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Molecular Characterization of Coprophilous Fungal Communities Reveals Sequences Related to Root-Associated Fungal Endophytes

The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve untranslated exon. To increase the value of the HRY gene as a genetic marker and to assess ...

1994-03-01

18

British Library Electronic Table of Contents (United Kingdom)

This paper reports the use of molecular methods to characterize the coprophilous fungal communities (CFC) that inhabit the dung of four species of mammalian herbivores at two sites, Sevilleta National Wildlife Refuge (SNWR) in New Mexico and Wind Cave National Park (WCNP) in South Dakota. Results reveal that CFC from domesticated cattle (Bos taurus) at SNWR, and bison (Bison bison) and black-tailed prairie dogs (Cynomys ludovicianus) at WCNP were diverse but dominated primarily by members within eight taxonomic orders, including the rarely cultured and anaerobic order Neocallimastigales. In addition, 7.7% (138 of 1,788) of the sequences obtained from all dung samples were at least 97% similar to root-associated fungal (RAF) sequences previously described from blue grama (Bouteloua gracilis...

2011-01-01

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Phytoremediation potential of Portulaca grandiflora Hook. (Moss-Rose) in degrading a sulfonated diazo reactive dye Navy Blue HE2R (Reactive Blue 172)

British Library Electronic Table of Contents (United Kingdom)

Wild and tissue cultured plants of Portulaca grandiflora Hook. have shown to be able to decolorize a sulfonated diazo dye Navy Blue HE2R (NBHE2R) up to 98% in 40h. A significant induction in the activities of lignin peroxidase, tyrosinase and DCIP reductase was observed in the roots during dye decolorization. The wild plants and tissue cultures could independently decolorize and degrade NBHE2R into metabolites viz. N-benzylacetamide and 6-diazenyl-4-hydroxynaphthalene-2-sulfonic acid. A dye mixture and a textile effluent were also decolorized efficiently by P. grandiflora. The phytotoxicity study revealed reduction in the toxicity due to metabolites formed after dye degradation.

2011-01-01

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Determination of a various ions such as alkali metals in leaves, stems, roots and seeds of the radish and their distribution

Role of Cytokinin and Auxin in Shaping Root Architecture: Regulating Vascular Differentiation, Lateral Root Initiation, Root Apical Dominance and Root Gravitropism

Determination, uptake and distribution of various ions such as alkali metals in three different parts (leaf, stem and root) and seeds of radish (Kaiware daikon) were examined using flame emission spectrometry and ICP-AES. In order to examine the influence of concentration alkali metal ion concentration in the radish culture solution on the uptake and distribution of these metals, the radish was grown at pH 5.6 in solutions containing alkali metal chloride at concentrations ranging from 10{sup -5} to 10{sup -1} mol dm{sup -3}. When the radish were grown in culture solution with alkali metal ions of low concentrations (10{sup -5} and 10{sup -4} mol dm{sup -3}), Na, K, Rb and trace Li were detected in leaves, stems and roots while Cs was scarcely detected. However, the contents of Na, K, Li in these organs were the same as those in radish cultivated in pure water. An increase of Rb uptake was observed with ...

1995-05-01

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Effect of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Nanofiber Matrices Cocultured With Hair Follicular Epithelial and Dermal Cells for Biological Wound Dressing

• Background and Aims Development and architecture of plant roots are regulated by phytohormones. Cytokinin (CK), synthesized in the root cap, promotes cytokinesis, vascular...Full Text Available

2006-05-01

22

British Library Electronic Table of Contents (United Kingdom)

Abstract: We tested the effects on the early-stage wound healing of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber matrices cultured with hair follicular cells. PHBV only, PHBV/collagen, and PHBV/gelatin at a 7/3 weight ratio were produced by electrospinning, and their in vitro cell culture and in vivo wound healing as biological dressings were examined. In cell attachment and growth on matrices, dermal sheath (DS) cells attached to hydrophilic PHBV/collagen and PHBV/gelatin faster than hydrophobic PHBV at the early incubation stage (up to 6 h). From 6- to 24-h incubation, PHBV/collagen showed the best results in cell culture. Furthermore, PHBV/collagen cocultured for 3-5 days with DS and epithelial outer root sheath (ORS) cells expressed more extracellular materials, such a...

2007-01-01

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The TofRoot project - status and perspectives

The effect of potassium nutrition on "1"3"7Cs uptake in two upland species

No abstract prepared.

2002-03-01

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Lower order roots more palatable to herbivores: a case study with two temperate tree species

Agrostis capillaris (Agrostis) and Calluna vulgaris (Calluna), two species with differing phenologies and widespread presence in upland areas of Britain where high Chernobyl fallout occurred, were grown in pot culture with varying concentrations of potassium in the rooting medium. Tissue content of potassium increased with increasing supply in both species. Roots, excised from these plants, were placed in a solution of "1"3"7Cs-labelled caesium chloride for 15 min to determine uptake potential. There were clear negative relationships between the rate of uptake of "1"3"7Cs by both species and (a) the concentration of potassium supplied and (b) plant issue potassium concentrations. With Agrotis, there was an approximately ten-fold difference in "1"3"7Cs uptake between potassium-deficient and optimum plants; with Calluna, it was approximately eight-fold. These results demonstrate the suppression of "1"3"7Cs uptake into plants ...

25

British Library Electronic Table of Contents (United Kingdom)

Determining which kinds of roots are likely to be consumed by root herbivores may improve our understanding of the mechanistic control on fine root dynamics. Here, we tested the hypothesis that root herbivores prefer to consume the distal lower order roots in their branching networks. Insecticide was applied to soil to quantify effects of root herbivores on root biomass and production in the first five orders (the distal roots numbered as first-order) in Fraxinus mandshurica and Larix gmelinii plantations from May 2008 to July 2009. Root morphology, chemistry, anatomy and physiology were measured simultaneously across branching orders. Among the first five order roots, significant consumptions by herbivores were found only for the two distal lower order roots ...

2011-01-01

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Teachers' understanding of culture and transference of culture : two investigations in the Norwegian region, Telemark, with special reference to cultural heritage

Norwegian Open Research Archives (NORA)

Full Text Available

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Influenza Vaccines

International Science & Technology Center (ISTC)

Development of the Culture Influenza Vaccine

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Applied and fundamental aspects of plant cell, tissue, and organ culture

A Study of Food Culture in South-Eastern Asia-about Dietetic Culture in Indonesia-

Applied and fundamental aspects of plant cell, tissue, and organ culture

1977-01-01

29

Modern microbialites and their environmental significance, Meiji reef atoll, Nansha (Spratly) Islands, South China Sea

Full Text Available

1992-03-01

30

British Library Electronic Table of Contents (United Kingdom)

Meiji (Mischief) coral atoll, in Nansha (Spratly) Islands, South China Sea, consists of an annular reef rim surrounding a central lagoon. On the atoll rim there are either protuberant ?motu? (small coral patch reefs on the rim of atoll) islets or lower sandy cays that contain modern microbialite deposits on the corals in pinnacles and surrounding bottoms of the atoll. Microbialites, including villiform, hairy, and thin spine growth forms, as well as gelatinous masses, mats and encrustation, developed on coral colonies and atoll rim sediments between 0 and 15 m deep-water settings. The microbialites were produced by natural populations of filamentous cyanobacteria and grew on (1) bulbous corals together with Acropora sp., (2) on massive colonies of Galaxea fascicularis, (3) on dead Montipor...

2008-01-01

31

Mechanisms mediating the trophic effect of nerves during vertebrate limb regeneration

Sensing the physical and nutritional status of the root growth environment

Salamanders regenerate their appendages after amputation and nerves are required for this process. Experiments were designed to test the idea that one way nerves could affect blastema cell proliferation is by influencing the metabolism of extracellular matrix (ECM) components and to identify neurotrophic factors which promote blastema cell mitosis. Temporal and spatial differences of glycosaminoglycans (GAGs) synthesis is innervated and denervated limbs were examined. Hyaluronic acid (HA) was found to be the major GAG produced during the proliferative period and chondroitin sulfate during differentiation. Denervation reduced synthesis of both these components by half. Dorsal root ganglia and fibroblast growth factor (FGF), a brain-derived mitogen, similarly doubled GAG synthesis in cultured blastemas, the FGF-effect being primarily on HA production. Histochemical and autoradiographical results confirmed the biochemical data. Autoradiography of ...

1986-01-01

32

Environmental Research Database

DescriptionAbstract of Research Proposal The root environment has a major effect on crop growth, both directly through the supply of water and nutrients to the shoot, and indirectly through root to shoot signalling. Better management of crop root systems through agronomic and genetic means has the potential to improve the efficiency of water and nutrient uptake, and limit root restrictions to crop growth. However, progress in this area is currently limited by the lack of sensors for in situ estimates of [continued...

2004-01-30

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Electrical Resistances of Corn Root Segments ¹²

Carbon Dioxide Fixation in Soybean Roots and Nodules

Longitudinal electrical resistances have been measured on 2-centimeter segments of corn (Zea mays L.) roots, cut at varying distances from the root apex. The segment resistances vary...Full Text Available

1976-02-01

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Cross-Cultural Studies of Personality Traits and their Relevance to Psychiatry

These studies demonstrate that soybean (Merr) roots and nodules possess an active system for fixing CO₂. The maximum rates of CO₂ fixation observed for roots and nodules of intact...Full Text Available

1981-04-01

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Potassium Transport in Corn Roots 1

AimsThis article provides a brief review of recent cross-cultural research on personality traits at both individual and culture levels, highlighting the relevance...Full Text Available

2006-01-01

36

At the Root of the Wood Wide Web

The relative transport capabilities of the cells of the root periphery and cortex were investigated using a variety of experimental techniques. Brief (30 seconds to 1 minute) exposures with the penetrating...Full Text Available

1983-10-01

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'Sixth root of unity' and Feynman diagrams: hypergeometric function approach point of view

Arbuscular mycorrhizal (AM) fungi are mutualistic symbionts living in the roots of 80% of land plant species, and developing extensive, below-ground extraradical hyphae fundamental for the uptake of...Full Text Available

2006-01-01

38

Safety culture development at Daya Bay NPP

We briefly discuss the transcendental constants generated through the #epsilon# expansion of generalized hypergeometric functions and their interrelation with the 'sixth root of unity.'

2010-04-25

39

Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens-Mediated Transformation of Hybrid Aspen and Poplar Clones

From view on Organization Behavior theory, the concept, development and affecting factors of safety culture are introduced. The focuses are on the establishment, development and management practice for safety culture at Daya Bay NPP. A strong safety culture, also demonstrated, has contributed greatly to improving performance at Daya Bay

2001-12-01

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DEVICE FOR TISSUE CULTURE, PROTEIN EXTRACTION AND PROTEIN CONCENTRATION

Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures...Full Text Available

1990-07-01

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J-STORE (Japan)

Full Text Available

2007-12-28

42

A study on multi-cultural family wives adapting to Korean cuisine and dietary patterns

A study on dietary culture in Nara Dynasty in JAPAN

With the increase in multi-cultural families, Korea is seeing a rapid increase in immigrated housewives, who are closely related to food culture. However, studies for the diet of multi-cultural families,...Full Text Available

2010-10-01

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University of Central Lancashire - Facilities

Full Text Available

1997-06-01

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Wastenet

...specialised laboratories for pharmaceutics, tissue culture and molecular biology Excellent Laboratory Facilities Tissue Culture Facilities Molecular Biology Laboratory ...

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Pushing the envelope for cultural appropriateness: does evidence support cultural tailoring in type 2 diabetes interventions for Mexican American adults?

Culture study on the hybrid by interspecific crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), two dioecious species in culture

PMID:21343599

2011-02-22

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International workshop on 'in vitro' culture of forest tree species. Colloque international sur la culture 'in vitro' des essences forestieres

Full Text Available

2011-03-01

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The Germination, Cover View and Root Potential Properties of Plants within CSG Planting Block by Mixture Seeding

Some 43 papers were presented in 4 sections: Organ and bud culture of tree species (24 papers); Cell suspension and callus culture of tree species (11); Culture of haploid tissue of tree species and production of wood homozygotes (3); and Isolation, culture and somatic fusion of protoplasts as a means of somatic hybridization and genetic engineering (5).

1983-01-01

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Optimization of Enzymatic Pretreatment for the Production of Fermented Ginseng using Leaves, Stems and Roots of Ginseng

Full Text Available

2010-07-01

49

European Society for Evolutionary Biology meeting. Tracing the common roots of antibiotic resistance.

Full Text Available

2010-03-01

50

Fresh root decomposition pattern of two contrasting tree species from temperate agroforestry systems: effects of root diameter and nitrogen enrichment of soil

PMID:21921169

2011-09-16

51

British Library Electronic Table of Contents (United Kingdom)

Fresh tree root decomposition induced by tillage is an important source of soil nutrients in agroforestry systems. Here we examined the effects of tree species, root size and soil N enrichment on fresh root decomposition under laboratory conditions. Fresh roots with two diameters (<2 and 2?5?mm) of Populus euramericana cv. ?N3016? (poplar) and Pinus tabulaeformis (pine) collected from agroforestry systems in Northeast China were used in the experiment. For each root treatment, four N levels (0, 50, 100 and 150??g?N g?1 soil) were added. We recognized N concentration and C/N ratio as the root quality variables, and determined decomposition rates as cumulative CO2 production and mass loss. Poplar roots had higher N concentration and lower C/N ratio and decomposed faster than pine roots, and ...

2011-01-01

52

Hyaluronate synthesis by synovial villi in organ culture. [Dogs

Vegetative propagation of Norway spruce by stem cuttings

Individual canine synovial villi were used to establish short-term synovial organ cultures. These villi incorporated /sup 3/H-glucosamine into highly-polymerized /sup 3/H-hyaluronic acid (/sup 3/H-HA), which was the only /sup 3/H-glycosaminoglycan identified in the culture medium. Some /sup 3/H-HA, and larger amounts of other /sup 3/H-glycosaminoglycans, were recovered from cultured tissues. Culture medium /sup 3/H-HA content was proportional to the surface area of cultured villi. Organ cultures of nonvillous synovium were compared with villi; nonvillous cultures synthesized less /sup 3/H-HA per mm2 of their synovial intimal surface than villi. These cultures complement cell culture techniques for in vitro studies of synovial lining cell function.

1983-06-01

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The influence of soil and coppice cycle on the rooting habit of short rotation poplar and willow coppice

Cuttings were taken from the upper part of the crowns of spruce trees 60-70 yr old in stands at 3 different altitudes in the Rila Mountains and 4 in the Vitosha Mountains in Bulgaria. The cuttings, of 1-yr, 2-yr and 3-yr shoots, were rooted in sand, perlite and peat. Rooting % generally decreased with increasing altitude of provenance, but cuttings from the very highest altitudes showed increased rooting %. In general, 1-yr cuttings gave the best rooting %, but in some provenances the 2-yr cuttings were best. Sand was clearly the best rooting substrate. (Refs. 11).

1981-01-01

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Rooting of hybrid clones of Populus tremula L. x P. tremuloides Michx. by stem cuttings derived from micropropagated plants

The increased demand for renewable energy sources has led to large areas of former agricultural land being proposed for short rotation coppice (SRC) establishment. Concerns expressed over the potential impacts of tree roots on buried archaeological evidence led to a study into the rooting habit of SRC. Roots were exposed in trenches dug within a variety of willow and poplar clonal stands grown on brown earth, pelosol, ground-water gley and surface-water gley soils. Root depths and diameters were recorded in each of the 33 trenches. In total, over 18,000 roots were measured on 264 coppice stools. The rotation length, species and stool location within a block were all found to influence the maximum size of root produced. Soil type had some influence on the root number and depth, but the pattern of root distribution down ...

2004-06-01

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Resolving conflicting safety cultures

Propagation costs could be cut by replacing part of the micropropagation process with steps involving more traditional techniques. This study explored possibilities for improving existing vegetative propagation techniques for aspen using stem cuttings obtained from micropropagated plants. Vegetative propagation through stem cuttings was studied in 10 micropropagated hybrid aspen clones (Populus tremula L. x P. tremuloides Michx). Cuttings containing one axillary bud were harvested from the same donor plants twice during the growing season: the first harvest in May and the second harvest in July. Rooting percentage was correlated positively with root length, number of roots and height of cutting plant but negatively with length of rooting. The average rooting percentage was 53% in the first harvest and 27% in second harvest. Indole-3-butyric acid treatments (1.2 mM) significantly ...

2001-07-01

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Nuclear safety culture star-class assessment system based BP neural network

Several nuclear power plant sites have been wounded in the crossfire between two distinct corporate cultures. The traditional utility culture lies on one side and that of the nuclear navy on the other. The two corporate cultures lead to different perceptions of open-quotes safety culture.close quotes This clash of safety cultures obscures a very important point about nuclear plant operations: Safety depends on organizational learning. Organizational learning provides the foundation for a perception of safety culture that transcends the conflict between utility and nuclear navy cultures. Corporate culture may be defined as the knowledge, attitudes, and beliefs shared by employees of a given company. Safety culture is the part of corporate culture concerning shared attitudes and ...

1993-06-20

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Automated motion estimation of root responses to sucrose in two Arabidopsis thaliana genotypes using confocal microscopy

In order to build the safety culture for nuclear power industry, it is important to evaluate the safety culture scientifically. Considering the traits of safety culture in the nuclear power industry, 24 safety culture assessment indexes are established from 4 aspects such as Safety consciousness, Safety attitude, Safety action and Safety actuality by using the SMART criteria. Safety culture star-class assessment criterion is presented and safety culture star-class assessment system is developed by using Visual Basic 6.0 and BP neural network. The system has a better generalization ability, and it can show exactly which phase the safety culture is in. Experimental results show that safety culture star-class assessment is practical and easy to perform. (authors)

2007-02-01

58

British Library Electronic Table of Contents (United Kingdom)

Root growth is a highly dynamic process influenced by genetic background and environment. This paper reports the development of R scripts that enable root growth kinematic analysis that complements a new motion analysis tool: PlantVis. Root growth of Arabidopsis thaliana expressing a plasma membrane targeted GFP (C24 and Columbia 35S:LTI6b-EGFP) was imaged using time-lapse confocal laser scanning microscopy. Displacement of individual pixels in the time-lapse sequences was estimated automatically by PlantVis, producing dense motion vector fields. R scripts were developed to extract kinematic growth parameters and report displacement to ?0.1 pixel. In contrast to other currently available tools, Plantvis-R delivered root velocity profiles without interpolation or averaging across the root s...

2011-01-01

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Changes in the extracellular matrix and glycosaminoglycan synthesis during the initiation of regeneration in adult newt forelimbs

Biochemical and ultrastructural processing of ["1"2"5I]epidermal growth factor in rat epidermis and hair follicles: accumulation of nuclear label

The extracellular matrix (ECM) of the distal tissues in a newt limb stump is completely reorganized in the 2-3-week period following amputation. In view of numerous in vitro studies showing that extracellular material influences cellular migration and proliferation, it is likely that the changes in the limb's ECM are important activities in the process leading to regeneration of such limbs. Using biochemical, autoradiographic, and histochemical techniques we studied temporal and spatial differences in the synthesis of glycosaminoglycans (GAGs) during the early, nerve-dependent phase of limb regeneration. Hyaluronic acid synthesis began with the onset of tissue dedifferentiation, became maximal within 1 weeks, and continued throughout the period of active cell proliferation. Chondroitin sulfate synthesis began somewhat later, increased steadily, and reached very high levels during chondrogenesis. During the first 10 days after amputation, distributions of sulfated and nonsulfated GAGs ...

1986-01-01

60

Biochemical and ultrastructural processing of (/sup 125/I)epidermal growth factor in rat epidermis and hair follicles: accumulation of nuclear label

Although the intracellular ultrastructural processing of epidermal growth factor (EGF) and its receptor have been described in cell culture systems, very few studies have examined this phenomenon in intact tissues. We have examined the ultrastructural and biochemical handling of ["1"2"5I]EGF in the epidermis and hair follicle bulb of intact, viable, 3- to 5-day-old rat skin the EGF receptor distribution of which has already been documented and in which EGF has been shown to be biologically active. After incubation of explants with 10 nM ["1"2"5I]EGF for 2.5 h at 25 degrees or 37 degrees C, radiolabel was detected over the basal cells of the epidermis and hair follicle outer root sheath, confirming previous light microscope observations. More specifically, silver grains were observed near coated and uncoated plasma membrane and coated membrane invaginations, Golgi apparatus, lysosomal structures, and nuclei. Sodium azide inhibited ...

1987-01-01

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61

Regulation of Embryo Dormancy by Manipulation of Abscisic Acid in Kernels and Associated Cob Tissue of Zea mays L. Cultured in Vitro¹

Although the intracellular ultrastructural processing of epidermal growth factor (EGF) and its receptor have been described in cell culture systems, very few studies have examined this phenomenon in intact tissues. We have examined the ultrastructural and biochemical handling of (/sup 125/I)EGF in the epidermis and hair follicle bulb of intact, viable, 3- to 5-day-old rat skin the EGF receptor distribution of which has already been documented and in which EGF has been shown to be biologically active. After incubation of explants with 10 nM (/sup 125/I)EGF for 2.5 h at 25 degrees or 37 degrees C, radiolabel was detected over the basal cells of the epidermis and hair follicle outer root sheath, confirming previous light microscope observations. More specifically, silver grains were observed near coated and uncoated plasma membrane and coated membrane invaginations, Golgi apparatus, lysosomal structures, and nuclei. Sodium azide inhibited ...

1987-03-01

62

Mass Culture of Subanguina picridis and Its Bioherbicidal Efficacy on Acroptilon repens

Sectors of Zea mays cobs, with and without kernels were cultured in vitro in the presence and absence of fluridone. Cultured kernels, cob tissue, and embryos developed...Full Text Available

1989-09-01

63

Effects of pH, Temperature, and Nutrients on Propionate Degradation by a Methanogenic Enrichment Culture

A Russian knapweed (Acroptilon repens) shoot culture system, initiated from shoot tip culture, was used to generate a source of host plant tissue for the rearing of the nematode Subanguina...Full Text Available

1993-03-01

64

Effects of Protons and HZE Particles on Glutamate Transport in Astrocytes, Neurons and Mixed Cultures

Enrichment cultures were used to determine the conditions promoting fastest methanogenic propionate degradation and growth by adapting the cultures to various physical and chemical conditions and measuring...Full Text Available

1987-07-01

65

Cultural Variation in Vigilance and Precaution Themes.

... ions. MATERIALS AND METHODS Cell Culture Culture of NT2/D1 cells was carried out as described previously (18, 19). NT2 cells were plated at a density of 2. ... ...

66

Comprehensive Analysis of Blood Culture Performed at Nine University Hospitals in Korea

Determine the degree to which different precautionary themes are dominant in various target populations and how these themes are acquired. Assess cultural signals of potential danger: are there different kinds of potential danger in different cultures. Do...

2009-01-01

67

Comparison of Indoor Mercury Vapor in Common Areas of Residential Buildings with Outdoor Levels in a Community Where Mercury Is Used for Cultural Purposes

BackgroundOptimal blood culture performance is critical for successful diagnosis and treatment of sepsis. To understand the status of blood culture, we investigated several aspects...Full Text Available

2011-04-01

68

Sucrose Uptake by Sugar Beet Tap Root Tissue ¹

Elemental mercury has been imbued with magical properties for millennia, and various cultures use elemental mercury in a variety of superstitious and cultural practices, raising health concerns for...Full Text Available

2006-01-01

69

Root Suberin Forms an Extracellular Barrier That Affects Water Relations and Mineral Nutrition in Arabidopsis

Sucrose uptake by discs of mature sugar beet root tissue incubated in [¹⁴C]-sucrose exhibited nonsaturating kinetics over the concentration range of 1 to 500 millimolar. Uptake was inhibited...Full Text Available

1979-11-01

70

Pseudomonas aeruginosa-Plant Root Interactions. Pathogenicity, Biofilm Formation, and Root Exudation1

Though central to our understanding of how roots perform their vital function of scavenging water and solutes from the soil, no direct genetic evidence currently exists to support the foundational model...Full Text Available

2009-05-01

71

Partial purification and some properties of a cholinesterase from bush bean (Phaseolus vulgaris L.) roots.

Pseudomonas aeruginosa is an opportunistic human pathogen capable of forming a biofilm under physiological conditions that contributes to its persistence despite long-term treatment...Full Text Available

2004-01-01

72

Partial Purification of Digitonin-Solubilized ?-Glucan Synthase from Red Beet Root ¹

A cholinesterase was partially purified from bush bean (Phaseolus vulgaris L.) roots by using acridinium-based ligand affinity chromatography. The procedure gave a 78-fold increase in specific activity,...Full Text Available

1978-12-01

73

Partial Characterization of Cadmium-Binding Protein from Roots of Tomato ¹

An enriched glucan synthase fraction was obtained from red beet root microsomes by sequential extraction with the detergents 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate and digitonin....Full Text Available

1987-04-01

74

Organ-Specific Invertase Deficiency in the Primary Root of an Inbred Maize Line ¹

Cd-binding protein was extracted from tomato roots and purified on QAE-Sephadex A-25 and on Sephadex G-75 in 1 molar KCl buffer. The protein preparation was light brown and contained predominantly Cd...Full Text Available

1986-07-01

75

Nitrate Reduction by Roots of Soybean (Glycine max [L.] Merr.) Seedlings

An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble)...Full Text Available

1991-10-01

76

Mild salinity stimulates a stress-induced morphogenic response in Arabidopsis thaliana roots

Studies were conducted with 9 to 12 day-old soybean (Glycine max [L.] Merr. cv. Williams) seedlings to determine the contribution of roots to whole plant NO₃⁻...Full Text Available

1982-06-01

77

Isolation, Purification, and Characterization of an Endogenous Root-promoting Factor Obtained from Basal Sections of Pear Hardwood Cuttings

Plant roots exhibit remarkable developmental plasticity in response to local soil conditions. It is shown here that mild salt stress stimulates a stress-induced morphogenic response (SIMR) in Arabidopsis...Full Text Available

2010-01-01

78

Inhibitory Action of Auxin on Root Elongation Not Mediated by Ethylene

Basal segments taken from Old Home and Bartlett pear hardwood cuttings collected at intervals during the rooting period in September were extracted with ethanol and fractionated by paper chromatography...Full Text Available

1967-04-01

79

Induction of Jasmonate Biosynthesis in Arbuscular Mycorrhizal Barley Roots12

The inhibitory effects of indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) on elongation growth of pea (Pisum sativum L.) seedling roots were investigated...Full Text Available

1989-09-01

80

Diversity and activity of sugar transporters in nematode-induced root syncytia

Colonization of barley (Hordeum vulgare cv Salome) roots by an arbuscular mycorrhizal fungus, Glomus intraradices Schenck & Smith, leads to elevated levels...Full Text Available

2002-11-01

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81

Detection of an arbuscular mycorrhizal fungus in roots of different plant species with the PCR.

The plant-parasitic nematode Heterodera schachtii stimulates plant root cells to form syncytial feeding structures which synthesize all nutrients required for successful nematode development....Full Text Available

2009-07-01

82

The Secretion of ?-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro

PCR was used with the primer pair VANS1-NS21 to detect the arbuscular mycorrhizal fungus Glomus intraradices (commercial inoculum source) on roots of lettuce, zinnia, leek, pepper, and endive plants....Full Text Available

1995-07-01

83

Sugarcane Culture and Syrup Production.

Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like...Full Text Available

1974-03-01

84

Culture of cells from beagles with bronchioloalveolar carcinoma

The publication gives the basic information needed by growers of syrup sugarcane. It includes descriptions of the sugarcane plant characteristics needed in syrup varieties, the best varieties, cultural requirements and practices, and the diseases and inse...

1988-01-01

85

Cell cultures were prepared from lung tumors occurring in beagles following exposure to inhaled plutonium. Morphologic and growth characteristics of two of these cell lines are described.

1977-05-01

86

Culture and cooperation

CONSTRAINTS TO CRASSOSTREA ARIAKENSIS AQUACULTURE: SEASON AND METHOD OF CULTURE STRONGLY INFLUENCE SUCCESS OF GROW-OUT

Does the cultural background influence the success with which genetically unrelated individuals cooperate in social dilemma situations? In this paper, we provide an answer by analysing the data of Full Text Available

2010-09-12

87

... of culture season and method on benefits of oyster aquaculture may be obtained without appreciable risk of unplanned ... ...

88

lipids - GCMD

Rapid vegetative propagation of conifers

The data set consists of the following: - Biochemical analysis of Deschampsia leaves and root; - Chromatographic Analysis of membrane lipids (lipid profile) ...

89

Isolation and biological activity of lipids from licorice (Glycyrrhiza glabra) roots

Factors influencing the rooting-potential of stem cuttings of conifers were studied for the purpose of improving methods of rapid vegetative propagation. Within the bounds of hereditary limits, root regeneration depends, to a considerable extent, on the qualities of rooting-media, date of cutting excision, age and physiological conditions of the stock plant, age of the cutting-wood, its position on the plant, and chemical treatment. Hardwood cuttings, which have a relatively high content of endogenous auxin and carbohydrates, and morphological root initials, gave the best results. This method made it possible to increase the growth rate of cuttings 2.5-5 times that of cuttings taken from shoots of current growth. (Refs. 21).

1981-04-01

90

British Library Electronic Table of Contents (United Kingdom)

Hexane extract of licorice (Glycyrrhiza glabra L.) roots was obtained and investigated. Hydrocarbons, sterol ethers, triacylglycerides, free fatty acids, and free sterols were identified. The extract contains 70% neutral and 30% polar lipids. It is established that the lipid fraction of licorice roots is more effective than the analogous fraction of rosehip oil in stimulating the reparative regeneration of skin. In addition, this fraction also exhibits pronounced antiinflammatory and antiulcer effects, while being virtually nontoxic. Based on these results, the lipid fraction of licorice roots can be recommended as a parent substance for creating effective preparations in various medicinal forms.

2007-01-01

91

Antioxidant Activity from Different Root Parts of 6-year-old Panax ginseng C.A. Meyer (Yun-poong)

Electrophysiological differences between nociceptive and non-nociceptive dorsal root ganglion neurones in the rat in vivo

Full Text Available

2011-04-01

92

Characterization and Rooting Ability of Indole-3-Butyric Acid Conjugates Formed during Rooting of Mung Bean Cuttings ¹

Intracellular recordings were made from 1022 somatic lumbar dorsal root ganglion (DRG) neurones in anaesthetized adult rats, classified from dorsal root conduction velocities (CVs) as C, Aδ...Full Text Available

2005-06-15

93

Suitability of weed species prevailing in Spanish vineyards as hosts for root-knot nematodes

Indole-3-butyric acid (IBA) is rapidly metabolized by mung bean cuttings during rooting. Twenty-four hours after application, less than 20% of the applied IBA remained in the free form and its level...Full Text Available

1989-11-01

94

British Library Electronic Table of Contents (United Kingdom)

Commercial vineyards in southern Spain were surveyed and sampled during October to December 2004 to determine the extent to which common weeds present were suitable hosts of root-knot nematodes infesting soils of those vineyards. Seven weed species commonly growing in grapevine soils in southern Spain were found infected by either Meloidogyne incognita or M. javanica: Amaranthus retroflexus (redroot pigweed), Anchusa azurea (ox-tongue), Chenopodium album (goosefoot), Erodium moschatum (musk stork?s bill), Malva rotundifolia (low mallow), Sinapis alba (white mustard), and Solanum nigrum (black nightshade). The host suitability of the weeds to root-knot nematodes was evaluated on the basis of root galling severity and nematode population densities in soil and roots. Also, the host?parasite r...

2008-01-01

95

Selective vulnerability of dorsal root ganglia neurons in experimental rabies after peripheral inoculation of CVS-11 in adult mice

British Library Electronic Table of Contents (United Kingdom)

The involvement of dorsal root ganglia was studied in an in vivo model of experimental rabies virus infection using the challenge virus standard (CVS-11) strain. Dorsal root ganglia neurons infected with CVS in vitro show prolonged survival and few morphological changes, and are commonly used to study the infection. It has been established that after peripheral inoculation of mice with CVS the brain and spinal cord show relatively few neurodegenerative changes, but detailed studies of pathological changes in dorsal root ganglia have not previously been performed in this in vivo experimental model. In this study, adult ICR mice were inoculated in the right hindlimb footpad with CVS. Spinal cords and dorsal root ganglia were evaluated at serial time points for histopathological and ultrastru...

2009-01-01

96

Root Hairs Play a Key Role in the Endophytic Colonization of Olive Roots by Pseudomonas spp. with Biocontrol Activity

British Library Electronic Table of Contents (United Kingdom)

The use of indigenous bacterial root endophytes with biocontrol activity against soil-borne phytopathogens is an environmentally-friendly and ecologically-efficient action within an integrated disease management framework. The earliest steps of olive root colonization by Pseudomonas fluorescens PICF7 and Pseudomonas putida PICP2, effective biocontrol agents (BCAs) against Verticillium wilt of olive (Olea europaea L.) caused by the fungus Verticillium dahliae Kleb., are here described. A gnotobiotic study system using in vitro propagated olive plants, differential fluorescent-protein tagging of bacteria, and confocal laser scanning microscopy analysis have been successfully used to examine olive roots?Pseudomonas spp. interactions at the single-cell level. In vivo simultaneous visualization...

2011-01-01

97

Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

British Library Electronic Table of Contents (United Kingdom)

To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-...

2011-01-01

98

Amelioration of phytotoxic effects of Cd on mung bean seedlings by gluconic acid secreting rhizobacterium Enterobacter asburiae PSI3 and implication of role of organic acid

British Library Electronic Table of Contents (United Kingdom)

Mung bean seedlings inoculated with Enterobacter asburiae PSI3, a gluconic acid-producing rhizosphere isolate, enhanced plant growth in the presence of phytotoxic levels of Cd2+ in gnotobiotic pot experiments as compared to the uninoculated Cd-treated plants. Addition of organic acids to Cd-stressed seedlings promoted root elongation. Hematoxylin competition assays showed that organic acids could displace Cd2+ from the Cd2+: hematoxylin complex in the same order of effectiveness as was found for restoration of root net elongation viz. oxalate?>?malate?>?succinate while gluconate was effective at higher concentrations. Root associated Cd2+, assessed by hematoxylin staining of roots was found to be reduced when roots were treated with organic acid. Cd stress increased antioxidant enzymes suc...

2008-01-01

99

Radiation effects on the shoot tip culture of chrysanthemum

Predicting results of mycobacterial culture on sputum smear reversion after anti-tuberculous treatment: a case control study.

Japanese (Mar 1974). Japan Mabuchi, Toshio Kuwada, Hikaru . Kagawa Univ.,

100

Parallel expression of synaptophysin and evoked neurotransmitter release during development of cultured neurons

PMID:20205743

2010-03-06

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101

DEFF Research Database (Denmark)

Udgivelsesdato: 1991-null

1991-01-01

102

Organizational Culture and Changes of Command

Optimization of Culture Condition for the Hydrocinnamic Acid Production from Bacillus subtilis IJ-31

... The OER Support Form and NCOER Counselling Worksheet provide a way to match individual and organizational goals. ...

1991-04-26

103

Influence of Ethylene Oxide Fume upon Chromaticity of Cultural Material

Full Text Available

2005-09-01

104

Culture Conditions of Geobacillus kaustophilus DSM 7263 for Production of Thermophilic Extracellular Lipase

Full Text Available

2002-12-01

105

Comparative Study on Protein and Amino Acid Composition of Wild and Cultured Eel

Full Text Available

2010-06-01

106

Callus induction and plant regeneration from in vitro cultured petiole of 3 Gerbera cultivars.

Full Text Available

1986-01-01

107

Calcium-influx increases SOD1 aggregates via nitric oxide in cultured motor neurons.

Full Text Available

2004-10-01

108

A Comparative Study on the Dietary Culture Consciousness and Their Consumption Attitude of Traditional Foods between Korean and Japanese Women

Full Text Available

2007-10-01

109

From gene manipulation to forest establishment: shoot cultures of woody plants can be a central tool

Full Text Available

2003-08-01

110

Convoluted cells as a marker for maternal cell contamination in CVS cultures

Establishing germplasm of woody plants in microculture as shoot cultures has proved to be an effective method of overcoming many of the obstacles in working with these crops. Shoot cultures eliminate the changes associated with seasonal growth cycles and phase change and put large plants into a more manageable form. Well-established shoot cultures are central to successful clonal propagation systems for forest trees as well as to genetic improvement based on the use of cellular techniques such as protoplast manipulation. The physiological basis as to why tissues from shoot cultures are so readily manipulated is not well understood.

1985-05-01

111

DEFF Research Database (Denmark)

In order to identify cells of maternal origin in CVS cultures, tissue from 1st trimester abortions were cultivated and the cultures stained in situ for X-chromatin. Convoluted cells and maternal fibroblasts were found to be positive. By chromosome analysis of cultures from 105 diagnostic placenta biopsies, obtained by the transabdominal route, metaphases of maternal origin were found in nine cases. In eight of these cases colonies of convoluted cells were observed. We conclude that convoluted cells are of maternal origin and are a reliable marker for maternal cell contamination in CVS cultures.

1987-01-01

112

Culture-area relation in Axelrod's model for culture dissemination

A cultural research approach to instrument development: the case of breast and cervical cancer screening among Latino and Anglo women.

A salient facet of Axelrod's model for culture dissemination or social influence is the existence of many multicultural absorbing states. The dependence of the number $C$ of different, coexisting cultures on the size $A$ of the territory or, equivalently, on the number of agents -- the culture-area relation -- is investigated through extensive simulations. This relation exhibits a strong qualitative dependence on the two parameters of the model, namely, the number $F$ of culture features and the number $q$ of values that each feature can take on. We find that a non-monotonous culture-area relation, for which the number of cultures decreases when the area grows beyond a certain size, occurs for $q$ smaller than a threshold value $q_c = q_c (F)$ provided that $F \\geq 3$. In the limit of infinite area, this threshold value signals the onset of a discontinuous ...

2009-01-01

113

Single leptoquark production at e"+e- and #gamma##gamma# colliders

To illustrate the implementation of a bottom-up approach to the study of culture in health disparities, this article describes the development of a cultural cancer screening scale (CCSS) using mixed methodologies. The aim was to identify cultural factors relevant to breast and cervical cancer screening, develop an instrument to assess them and examine its preliminary psychometric properties among Latin American (Latino) and non-Latino White (Anglo) women in Southern California. Seventy-eight Latino and Anglo women participated in semi-structured interviews, which were content coded based on Triandis' methods for the analysis of subjective culture. Based on the emerging cultural elements, items relevant to cancer screening were developed and pilot tested with 161 participants. After the instrument was refined, 314 Latino and Anglo women from various socioeconomic backgrounds ...

2010-09-23

114

Is culture a determinant of financial development?

We consider single production of leptoquarks (LQ's) at e"+e- and #gamma##gamma# colliders, for two values of the center-of-mass energy: #sq root#s =500 GeV and 1 TeV. We find that LQ's which couple within the first generation are observable for LQ masses almost up to the kinematic limit, both at e"+e- and #gamma##gamma# colliders, for an LQ coupling strength equal to #alpha#_e_m. The cross sections for single production of second- and third-generation LQ's at e"+e- colliders are too small to be observable. In #gamma##gamma# collisions, on the other hand, second-generation LQ's with masses much larger than #sq root#s /2 can be detected. However, third-generation LQ's can be seen at #gamma##gamma# colliders only for masses at most #approx# #sq root#s /2, making their observation more probable via the pair production mechanism.

115

British Library Electronic Table of Contents (United Kingdom)

This article investigates the missing link in the literature - whether informal institutions, or what is known as culture, can affect the level of financial development for a country? Our hypothesis stresses that the cultural dimensions of a country can have an impact on its financial set-up. We consider multiple dimensions of culture, identified in the literature by Tabellini (2008), to test our hypothesis. As culture evolves in the form of greater trust, control and other traits, individuals' attitudes towards financial market change, and they engage in greater financial transactions. This, in turn, leads to better financial development. Using quantile estimation technique for a cross section of 90 countries, we find that culture significantly influences the level of financial developmen...

2012-01-01

116

Cultural inheritance and diversification of diet in variable environments

British Library Electronic Table of Contents (United Kingdom)

Both cultural inheritance and cultural diversification of diets may play an important role in animal evolution. Here we studied how diet innovation and cultural change relate to cultural inheritance in a changing environment. We did this by studying diet cultures in group foragers adapting to environmental change through learning, and the consequences this has for diet differentiation between groups. We used an individual-based model of `monkeys' that learn what to eat in a rich environment, and we changed resource species that are available in the environment. Relative to social influences on learning that arise spontaneously in groups, we found that more direct social learning, in the sense of observing another individual and copying what it eats, helps groups deal with high levels of en...

2009-01-01

117

Cubature Kalman smoothers

British Library Electronic Table of Contents (United Kingdom)

The cubature Kalman filter (CKF) is a relatively new addition to derivative-free approximate Bayesian filters built under the Gaussian assumption. This paper extends the CKF theory to address nonlinear smoothing problems; the resulting state estimator is named the fixed-interval cubature Kalman smoother (FI-CKS). Moreover, the FI-CKS is reformulated to propagate the square-root error covariances. Although algebraically equivalent to the FI-CKS, the square-root variant ensures reliable implementation when committed to embedded systems with fixed precision or when the inference problem itself is ill-conditioned. Finally, to validate the formulation, the square-root FI-CKS is applied to track a ballistic target on reentry.

2011-01-01

118

WALL PRESSURE FLUCTUATIONS UNDER TURBULENT ...

Unearthing the Phylogenetic Roots of Sleep

... necessary to allow for the effata nr the finits size of the oressure tranaducer on both the root mean square and power spectral density measurements ...

1963-04-01

119

Species and Organ Diversity in the Effects of Hydrogen Peroxide on Superoxide Dismutase Activity In Vitro

Why we sleep remains one of the enduring unanswered questions in biology. At its core, sleep can be defined behaviorally as a homeostatically regulated state of reduced movement and sensory...Full Text Available

2008-08-05

120

British Library Electronic Table of Contents (United Kingdom)

Abstract Superoxide dismutase (SOD) is ubiquitous in aerobic organisms and constitutes the first link in the enzyme scavenging system of reactive oxygen species. In the present study, species and organ diversity of SOD activity in a solution and in an in-gel assay system, as well as the effects of hydrogen peroxide (H2O2) on SOD activity, were investigated. In a solution assay system, SOD activity of jackfruit root, shoot, leaves, axes, and cotyledons, of maize embryos and endosperms, of mung bean leaves and seeds, of sacred lotus axes and cotyledons, and of rice and wheat leaves was increased by 1-15 mmol/L H2 O2. However, SOD activity in rice root and seeds, maize roots and leaves, mung bean roots and shoots, and wheat seeds was decreased by 1-15 mmol/L H2O2. The SOD activity of wheat ro...

2006-01-01

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121

Spatial distribution of arsenic and heavy metals in willow roots from a contaminated floodplain soil measured by X-ray fluorescence spectroscopy

British Library Electronic Table of Contents (United Kingdom)

Under changing redox conditions some plants create plaques at their root surface, which may affect the mobility and uptake of As and heavy metals but it is unknown to what extent this also holds true for willows in contaminated floodplain soils. Therefore, willow roots were sampled from a phytoremediation trial in the contaminated floodplain of the river Elbe (Germany), cryofixed, freeze-dried, and cross sections were mapped for the distribution of As, Ca, Cu, Fe, K, Mn, Ni, S and Zn by synchrotron based X-ray fluorescence spectroscopy. The elements Ca, Cu, Ni, S and Zn were concentrated in the aerenchymatic tissue, and not associated with Fe and Mn. Mixed Fe-Mn plaques covered the surface of the willow roots and As was accumulated in these plaques. The observed association pattern between...

2011-01-01

122

Single dilepton production at e"+e"-, e#gamma#, and #gamma##gamma# colliders

Root exudation of sugars, amino acids, and organic acids by maize as affected by nitrogen, phosphorus, potassium, and iron deficiency

We calculate the cross sections for the single production of doubly charged dileptons, both scalar and vector, at e"+e"-, e#gamma#, and #gamma##gamma# colliders at #sq root#s =500 GeV and 1 TeV. The e#gamma# mode is by far the most promising---dileptons whose coupling is as weak as #approx#10"-"4#alpha#_e_m can be observed, for masses virtually up to the kinematic limit. Dileptons of mass up to #sq root#s can also be seen in e"+e"- and #gamma##gamma# colliders, for couplings of order #alpha#_e_m. In all three colliders, most of the cross section comes from events in which the only particles detected are e"-e"- (or #mu#"-#mu#"- or #tau#"-#tau#"-), the decay products of the dilepton, yielding an unmistakable experimental signature.

123

British Library Electronic Table of Contents (United Kingdom)

Abstract Root exudates play a major role in the mobilization of sparingly soluble nutrients in the rhizosphere. Since the amount and composition of major metabolites in root exudates from one plant species have not yet been systematically compared under different nutrient deficiencies, relations between exudation patterns and the type of nutrient being deficient remain poorly understood. Comparing root exudates from axenically grown maize plants exposed to N, K, P, or Fe deficiency showed a higher release of glutamate, glucose, ribitol, and citrate from Fe-deficient plants, while P deficiency stimulated the release of -aminobutyric acid and carbohydrates. Potassium-starved plants released less sugars, in particular glycerol, ribitol, fructose, and maltose, while under N deficiency lower am...

2011-01-01

124

Root Cause Analysis, Tank Fire Problem, M1A1 Main Battle ...

Retinue of the beans roots growth by using neutron radiography technique

... ammunition is stored. Combustible cartridge cases could absorb moisture, swell, and not chamber properly. Additionally, moisture ...

1989-02-01

125

Agricultural practices frequently cause the development of a soil compacted layer below the surface. These compacted layers restrict the root penetration into deeper layers of soil, in search for water. It is proposed to monitor, using Non Destructive Test, the roots growth due to the planting of standard seeds in different agricultural soils, in function of their compactness and humidity. It will be used the neutrons beams derived from an irradiation channel called J-9 of the Reactor Argonauta (IEN/CNEN), so that the neutron radiographic images of the soil-plant system can be obtained. Each root can be evaluated for its ability to penetrate into compacted soil layers; this fact would mean an optimization of agricultural harvests. (author)

2002-08-11

126

Ras history

Orthognathic treatment case after severe root resorption in the early treatment stage

Although the roots of Ras sprouted from the rich history of retrovirus research, it was the discovery of mutationally activated RAS genes in human cancer in 1982 that stimulated an...Full Text Available

2010-07-01

127

British Library Electronic Table of Contents (United Kingdom)

This article presents an orthognathic treatment case after severe apical root resorption of maxillary anterior teeth using anterior segmental osteotomy. This case involved a 20-year-old female with the chief complaint of maxillary protruding and irregularly aligned mandibular anterior teeth. Her medical history showed no allergies or medical problems. The overbite was +3.0mm, and the overjet +3.0mm. After extraction of the four first premolars, a multi-bracket treatment was started. A severe root resorption of the maxillary anterior teeth was found 12 months after active treatment. The maxillary anterior segmental osteotomy was chosen as the compensatory treatment. The total treatment period was 2 years and 7 months. The post-retention panoramic radiograph showed no developmental root reso...

2009-01-01

128

Orphan GPCR research

Ocean Planet:perils-deforestation - SeaWiFS - NASA

Orphan G protein-coupled receptors (GPCRs) are receptors lacking endogenous ligands. Found by molecular biological analyses, they became the roots of reverse pharmacology, in which receptors are attempted...Full Text Available

2008-03-01

129

Indoleacetic Acid and Abscisic Acid Antagonism

Deforestation farther inland causes delayed reactions. When roots no longer hold soil in place, it ends up muddying streams, rivers, and coastal waters. ...

130

Changes in DNA content during rhizobial nodule development in Lupinus luteus L. 1

The effects of low concentrations (10 nm or less) of indoleacetic acid and abscisic acid on the phytochrome-mediated attachment of barley root (Hordeum vulgare L., var. Compana)...Full Text Available

1973-01-01

131

Canal-centering ability: An endodontic challenge

Cytophotometric measurements of DNA content after Feulgen's reaction and autoradiography after incubation with "3H-thymidine were performed to investigate the early stages of development of yellow lupin root nodule. The first response of the host plant to the contact with Rhizobium lupini was the enhancement of nuclear DNA endoreplication in root cortex cells. A conversion of the initially mixoploid nodule meristem into a diploid one during root nodule formation was found. Differentiation of bacteroid-containing tissue both from diploid and polyploid cells was observed. The highest content of nuclear DNA in the uninfected root cortex, nodule cortex and in bacteroid-containing tissue was 16 C. (author).

1989-01-01

132

Automated Eddy Current Inspection of Space Shuttle APV ...

During instrumentation of the root canal, it is important to develop a continuously tapered form and to maintain the original shape and position of the apical foramen. However, the presence of curvatures...Full Text Available

2009-01-01

133

Application of chloropicrin to Douglas-fir stumps to control laminated ...

... perform the inspection in the extremely limited space between adjacent blades and at the blade root, miniature eddy current probes were developed ...

134

Analysis of Rough Wall Turbulent Heating with Application to ...

Sep 1, 2011 ... Title: Application of chloropicrin to Douglas-fir stumps to control laminated root rot does not affect infection or growth of regeneration 16 growing ...

135

Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root

... roughness. Since T u'v' and q v'"F the heat transfer augmentation is the square root of the skin friction augmentation: - 1/2 (( St - ,Cf 0 ...

1979-01-01

136

British Library Electronic Table of Contents (United Kingdom)

Glycyrrhiza glabra root is one of the common traditional Chinese medicines and used as flavoring and sweetening agents for tobaccos, chewing gums, candies, toothpaste and beverages. While glycyrrhizin is one of the main components in the extract of G. glabra root and has been characterized, the other components have not been well characterized. The mechanism of growth activation of breast cancer MCF-7 cells, including the activation of Erk1/2 and Akt, and the transcriptional regulation of estrogen-responsive genes, was examined by means of sulforhodamine B, luciferase reporter gene, real-time RT-PCR and Western blotting assays after the induction of the cells with the extract of G. glabra root. The extract has similar activity to that induced by 17b-estradiol (E2), although glycyrrhizin di...

2007-01-01

137

Uptake and Intracellular Activity of Moxifloxacin in Human Neutrophils and Tissue-Cultured Epithelial Cells

Stimulation of albumin endocytosis by cationized ferritin in cultured aortic smooth muscle cells.

The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At...Full Text Available

1999-01-01

138

Requirements for Extraction of Polyribosomes from Plant Callus Cultures ¹

Anionic microdomains within the aortic smooth muscle cell (SMC) surface glycocalyx represent a potential barrier to the endocytosis of anionic plasma proteins. Cultured SMCs exposed briefly to cationized...Full Text Available

1985-12-01

139

Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division.

A procedure was developed to isolate polyribosomes from plant cell cultures. Tobacco callus extracted in 10 mm MgCl₂, 80 mm KCl, 250 mm sucrose, and 140 mm...Full Text Available

1977-05-01

140

Propagation of human embryonic and induced pluripotent stem cells in an indirect co-culture system

Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol...Full Text Available

1990-02-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations

We have developed and validated a microporous poly(ethylene terephthalate) membrane-based indirect co-culture system for human pluripotent stem cell (hPSC) propagation, which allows real-time...Full Text Available

2010-03-05

142

Political Culture and the Nature of Political Participation in Egypt.

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured...Full Text Available

2009-06-01

143

Nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia.

This thesis analyzes Egyptian political culture and the directive effect which it has upon political development, elite recruitment and the nature of political participation. The pervasive nature of Islam and the centrality of kinship remain the basic fac...

1980-01-01

144

Molecular and immunological comparison of human dihydropteridine reductase in liver, cultured fibroblasts and continuous lymphoid cells.

Forty-eight mutants unable to reduce nitrate were isolated from "cowpea" Rhizobium sp. strain 32Hl and examined for nitrogenase activity in culture. All but two of the mutants had nitrogenase activity...Full Text Available

1977-02-01

145

Model for phase III autografts of epidermal cells cultured on a collagen-proteoglycan biomatrix.

An antiserum was raised in a rabbit against highly purified human liver dihydropteridine reductase (EC 1.6.99.7). Dihydropteridine reductase from human liver, in human cultured fibroblasts and in continuous...Full Text Available

1981-07-01

146

Isolation of a bacterium resembling Pirellula species from primary tissue culture of the giant tiger prawn (Penaeus monodon).

The primary aim of this study was to develop a model system that uses epidermal cells (keratinocytes and accessory pigmented cells) cultured on a reconstituted basement membrane biomatrix for use in...Full Text Available

1989-10-01

147

Hanford Cultural Resources Laboratory annual report for fiscal year 1990

During attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (Penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin...Full Text Available

1991-11-01

148

Growth temperature reversibly modulates the virulence of Legionella pneumophila.

The Hanford Cultural Resources Laboratory (HCRL) was established by the US Department of Energy Field Office, Richland (RL) in 1987 as part of Pacific Northwest Laboratory. The HCRL provides support for managing the archaeological, historical, and cultural resources of the Hanford Site, Washington, in a manner consistent with federal statutes and regulations. This report summarizes activities of the HCRL during fiscal year (FY) 1990. The HCRL responsibilities have been set forth in the Hanford Cultural Resources Management Plan (HCRMP) as a prioritized list of tasks. The task list guided cultural resources management activities during FY 1990 and is the outline for this report. In order, these tasks were to (1) conduct cultural resource reviews, (2) develop an archaeological resources protection plan, (3) monitor the condition of known archaeological sites, (4) plan a curation ...

1991-11-01

149

Effects of Cycling Temperatures on Fiber Metabolism in Cultured Cotton Ovules ¹

In chemostat culture, the virulence of two strains of Legionella pneumophila was shown to be significantly (P < 0.05) reduced when the culture temperature was lowered from 37 to 24 degrees C....Full Text Available

1994-07-01

150

Discovering Networks of Perturbed Biological Processes in Hepatocyte Cultures

The effects of temperature on rates of cellulose synthesis, respiration, and long-term glucose uptake were investigated using cultured cotton ovules (Gossypium hirsutum L. cv Acala...Full Text Available

1992-10-01

151

Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp

The liver plays a vital role in glucose homeostasis, the synthesis of bile acids and the detoxification of foreign substances. Liver culture systems are widely used to test adverse effects of drugs...Full Text Available

152

Cultural beliefs that may discourage breastfeeding among Lebanese women: a qualitative analysis

A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility...Full Text Available

1990-11-01

153

Cultural and Contextual Influences on Parenting in Mexican American Families

BackgroundAlthough the health benefits of breastfeeding are well established, early introduction of formula remains a common practice. Cultural beliefs and practices can have an...Full Text Available

154

Cell-density-dependent Changes in the Metabolism of Chloronema Cell Cultures

Family stress theory can explain associations between contextual stressors and parenting. However, the theory has not been tested among Mexican Americans or expanded to include cultural-contextual...Full Text Available

2009-02-01

155

The Inhibition of Epileptogenesis During Status Epilepticus by Ginsenosides of Korean Red Ginseng and Ginseng Cell Culture (Dan25)

In the growing chloronema cell suspension cultures of the moss Funaria hygrometrica Hedw., activities of several enzymes have been found to be cell-density-dependent. Cyclic nucleotide...Full Text Available

1979-07-01

156

Studies on Persistence of Pesticides in Soils and Crops under Polyethylene Film Mulching Culture;IV. Persistence of Herbicides Alachlor, Pendimethalin and Diphenamid

Full Text Available

2007-09-01

157

Neuroprotective Activities of Some Medicinal Plants against Glutamate-induced Neurotoxicity in Primary Cultures of Rat Cortical Cells

Full Text Available

1988-06-01

158

Methods to improve the fertility of poinsettia

Full Text Available

2009-09-01

159

British Library Electronic Table of Contents (United Kingdom)

`Success' or `Red Splendor' plants propagated by shoot tip culture or embryogenesis had higher pollen germination than those propagated by cutting. All clones of `Pepride', `Peter Star' and `Nobel Star' propagated by cutting or by embryogenesis or shoot tip culture did not have viable pollen. `Peter Star' mutants through mutagenesis could recover pollen viability. When both parents were propagated by tissue culture, fruit set increased compared to when one or both parents were propagated by cuttings. Furthermore, the number of the harvested seeds doubled per pollination set, when the tissue culture parents were used instead of cutting-propagation plants.

2008-01-01

160

Educational research in Confucian cultural contexts: reflections on methodology

British Library Electronic Table of Contents (United Kingdom)

This paper engages in a critical discussion of the key features delineated by the other authors in this Special Issue thus serving as an end-piece for the issue. In particular it touches on three significant concepts. Firstly, it reinforces the importance of context, particularly cultural context in the conduct of research in Confucian cultural settings. Second, as an illustration, it offers a critical perspective on the cultural assumptions that underpin the development of Chinese higher education. Finally, it concludes with some reflections on how other papers in this Special Issue contribute to our understanding of research in CHC contexts.

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

161

Beyond the Flatline

British Library Electronic Table of Contents (United Kingdom)

Abstract High Post-Modernism with its understanding of the significance of the image anticipated the flattening of culture, first in advertising and the printed media, and more recently online. Sam Jacob of FAT speculates on architecture's relationship with the Internet, -a flatland of undifferentiated information-, and how Radical Post-Modernism might be best placed to resist the ubiquity of global culture while reanimating the social and cultural agenda of Modernism and Post-Modernism. Copyright 2011 John Wiley & Sons, Ltd.

2011-01-01

162

The microbiology of forest soils: a literature review

Spatial Distribution of Turgor and Root Growth at Low Water Potentials ¹

This report discusses the activities of two major groups of forest soil microorganisms, the bacteria and the fungi. Special attention is paid to their participation in the decay of major forest litter substrates, including leaves, branches and roots. The influence of bacteria and fungi in symbiotic associations with woody plant roots upon the cycles of carbon and nitrogen is described. The impacts of certain forest mamagement alternatives are assessed in terms of the creation of elimination of suitable environments for the activity of soil microorganisms. A bibliography is included. 507 refs., 1 tab.

1982-01-01

163

Safety analysis and license of rod drop time issue at Daya Bay Nuclear Power Plant

Spatial distributions of turgor and longitudinal growth were compared in primary roots of maize (Zea mays L. cv FR27 × FRMo 17) growing in vermiculite at high (−0.02...Full Text Available

1991-06-01

164

Proton-Transfer-Reaction Mass Spectrometry as a New Tool for Real Time Analysis of Root-Secreted Volatile Organic Compounds in Arabidopsis1

The rod drop time event of the Daya Bay Nuclear Power Plant is caused by the malfunction of the guide tube developed by Framatome. Three temporary solutions were implemented successively and the long term solution was found in the process of searching for the root cause. The different solutions and the root cause are introduced. The safety analysis and license of the solutions are mainly discussed. Experiences and lessons are drawn by summarizing the important items related to nuclear safety.

165

Potassium Loss and Changes in the Fine Structure of Corn Root Tips Induced by H-ion 1

Plant roots release about 5% to 20% of all photosynthetically-fixed carbon, and as a result create a carbon-rich environment for numerous rhizosphere organisms, including plant pathogens and symbiotic...Full Text Available

2004-05-01

166

Intracellular pH Regulation during NO₃^? Assimilation in Shoot and Roots of Ricinus communis

The effects of H, Ca, and anaerobiosis upon loss of K to ambient solutions, upon changes in the fine structure, and upon the respiration of corn root tissue were studied. In the pH range 5.5 to 8.0...Full Text Available

1966-12-01

167

Increased Endogenous Abscisic Acid Maintains Primary Root Growth and Inhibits Shoot Growth of Maize Seedlings at Low Water Potentials ¹

Ricinus communis L. was used to test the Dijkshoorn-Ben Zioni hypothesis that NO₃⁻ uptake by roots is regulated by NO₃⁻ assimilation...Full Text Available

1985-08-01

168

Heavy metals in industrial wastewater determined by radionuclide x-ray fluorescence analysis and their effects on Allium cepa root tip cells

Roots of maize (Zea mays L.) seedlings continue to grow at low water potentials that cause complete inhibition of shoot growth. In this study, we have investigated the role of abscisic...Full Text Available

1990-08-01

169

Effect of Lanthanum on Ion Absorption in Corn Roots ¹

Radionuclide x-ray fluorescence method was used in the determination of Cr, Ni and other heavy metals content in wastewater of an engineering plant taken both before and after chemical treatment. Toxic effects of the wastewaters using Allium cepa root tip cells were tested. (author) 8 refs.; 2 tabs.

1994-05-01

170

Effect of Exogenous Indole-3-Acetic Acid and Indole-3-Butyric Acid on Internal Levels of the Respective Auxins and Their Conjugation with Aspartic Acid during Adventitious Root Formation in Pea Cuttings

Short term (10 min) influx of ⁸⁶Rb-labeled potassium into corn (Zea mays L. WF9 × M14) root segments was inhibited by La (NO₃)₃ or LaCl₃....Full Text Available

1975-03-01

171

Characterization of Fatty Acid Biosynthesis in Isolated Pea Root Plastids ¹

The influence of exogenous indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) on the internal levels of these auxins was studied during the first 4 days of adventitious root formation in cuttings...Full Text Available

1991-07-01

172

Toroidal rotation braking with n = 1 magnetic perturbation field on JET

Fatty acid biosynthesis from Na[1-¹⁴C]acetate was characterized in plastids isolated from primary roots of 7-day-old germinating pea (Pisum sativum L.) seeds. Fatty acid...Full Text Available

1991-06-01

173

Root canal preparation with the NiTi systems K3, Mtwo and ProTaper

A strong toroidal rotation braking has been observed in plasmas with application of an n = 1 magnetic perturbation field on the JET tokamak. Calculation results from the momentum transport analysis show that the torque induced by the n = 1 perturbation field has a global profile. The maximal value of this torque is at the plasma core region (#rho# < 0.4) and it is about half of the neutral beam injection torque. The calculation shows that the plasma is mainly in the #nu#_-#sq root##nu# regime in the plasma core, but it is close to the transition between the 1/#nu# and #nu#_-#sq root##nu# regimes. The neoclassical toroidal viscosity (NTV) torque in the 1/#nu# and #nu#_-#sq root##nu# regimes is calculated. The observed torque is of a magnitude in between that of the NTV torque in the 1/#nu# and #nu#_-#sq root##nu# regimes. The NTV torque in the #nu#_-#sq root##nu# regimes is ...

2010-10-01

174

British Library Electronic Table of Contents (United Kingdom)

Abstract The aim of this paper was to examine the result of rotary root canal preparation with the nickel-titanium (NiTi) systems K3, ProTaper and Mtwo. One hundred and fifty curved artificial root canals and 60 mesial canals of human mandibular molars were selected. In the group of curved artificial canals, all canals were prepared to size 35/0.04 taper with the three systems. In the group of human mandibular molars, the teeth with mature root canals were radiographed with silver points inserted in bucco-lingual and in mesio-distal positions. In the artificial root group, one K3 instrument separated. Mtwo (20%) showed significantly (P = 0.003) less zips than K3 (46.9%) or ProTaper (50%). There were no significant differences in ledge and elbow formation. K3 and Mtwo had the lowest percent...

2007-01-01

175

Root Architecture of Jarrah (Eucalyptus marginata) Trees in Relation to Post-Mining Deep Ripping in Western Australia

British Library Electronic Table of Contents (United Kingdom)

Abstract This research aimed to investigate coarse root systems of Jarrah (Eucalyptus marginata Donn ex Sm.) trees at a 13-year-old restored bauxite mine site in south-western Australia. Excavations in an area with small trees (low-quality site) revealed that deep ripping equipment failed to penetrate the cemented lateritic subsoil, restricting coarse roots (roots >5 mm in diameter) to the top 0.5 m of the soil profile, resulting in fewer (1,344 stems/ha) and smaller (mean height 4.5 m) Jarrah trees. An adjacent area within the same pit (high-quality site), with a stand density of 3,256 stems/ha and a mean height of 8.0 m, had a kaolinitic clay subsoil which coarse roots penetrated to the average ripping depth (1.5 m). Trees at the low-quality site did not penetrate the subsoil with their ...

2007-01-01

176

Calcium sulfate crystallization along citrus root channels in a Florida soil exhibiting acid sulfate properties

Thermokinetic investigation of effects of carbon source on petroleum bacterial growth

The authors observed euhedral crystals in Manatee soil in a citrus grove in St. Lucie County, Florida. The material was identified as gypsum (CaSO/sub 4/ /times/ 2H/sub 2/O) using x-ray diffraction and infrared spectra. Photomicrography and scanning electron microscopy revealed that gypsum accumulated both in old root channels and within citrus root tissue of the Btg horizon. The subsurface horizons had elevated sulfate levels, a low initial pH, a drop (0.5 unit) in pH upon air-drying. Electrical conductivity paralleled the concentration of water-soluble sulfate. High levels of calcium and sulfate occurred for horizons above the water table. This accumulation is attributed to groundwater bearing these ions and subsequently discharging them to the overlying soil. Dead citrus roots appear to act as wicks to aid water transfer from lower to higher horizons. The roots and their empty channels provide spaces ...

1988-02-01

177

P2Y receptors on astrocytes and microglia mediate opposite effects in astroglial proliferation

The growth power-time curves of a strain of petroleum bacteria, B-2, in various kinds of cultures containing different kinds of carbon sources, glucose, n-tetradecane, n-hexadecane and n-octadecane, and different kinds of microemulsions have been determined by using a 2277 Thermal Activity Monitor. The curves showed a single peak for cultures containing a single carbon source, glucose, and two peaks for cultures containing two kinds of carbon sources, glucose and one of the n-alkanes. The first peak indicated that bacteria grew by consuming glucose and the second peak indicated that bacteria grew by consuming n-alkane. The curves were complex when the bacterium grows in a microemulsion culture. According to a kinetic equation of bacterial growth under limited conditions, the rate constants of bacterial growth were obtained. The results showed that the microemulsion culture was more ...

2002-02-07

178

British Library Electronic Table of Contents (United Kingdom)

Nucleotides released upon brain injury signal to astrocytes and microglia playing an important role in astrogliosis, but the participation of microglia in the purinergic modulation of astrogliosis is still unclear. Highly enriched astroglial cultures and co-cultures of astrocytes and microglia were used to investigate the influence of microglia in the modulation of astroglial proliferation mediated by nucleotides. In highly enriched astroglial cultures, adenosine-5?-triphosphate (ATP), adenosine 5?-O-(3-thio)-triphosphate (ATP?S), adenosine 5?-O-(3-thio)-diphosphate (ADP?S; 0.01?1?mM), and adenosine-5?-diphosphate (ADP; 0.1?1?mM) increased proliferation up to 382%, an effect abolished in co-cultures containing 8% of microglia. The loss of ATP proliferative effect in co-cultures is supporte...

2011-01-01

179

Malignancy without immortality? Cellular immortalization as a possible late event in melanoma progression

British Library Electronic Table of Contents (United Kingdom)

Summary Cell senescence is a permanent growth arrest following extended proliferation. Cultured cancer cells including metastatic melanoma cells often appear immortal (proliferate indefinitely), while uncultured benign nevi (moles) show senescence markers. Here, with new explantation methods, we investigated which classes of primary pigmented lesions are typically immortal. Nevi yielded a few proliferating cells, consistent with most nevus cells being senescent. No nevus culture (0/28) appeared immortal. Some thin and thick melanoma cultures proved immortal under these conditions, but surprisingly few (4/37). All arrested cultures displayed three senescence markers in some cells: -galactosidase, nuclear p16, and heterochromatic foci/aggregates. However, melanoma cultures also showed featur...

2011-01-01

180

Importance of serum source for the in vitro replicative senescence of human bone marrow derived mesenchymal stem cells

British Library Electronic Table of Contents (United Kingdom)

Abstract Human mesenchymal stem cells (hMSCs) may be used for therapeutic applications. Culture conditions such as the serum source may impact on cell quality and the onset of replicative senescence. We have examined the effect of culturing hMSCs in autologous serum (AS) versus fetal bovine serum (FBS) on factors involved in in vitro replicative senescence. hMSCs from four donors were cultured in 10% FBS or 10% AS until they reached senescence. Cells were harvested at early passage and near senescence to study factors known to be involved in cellular senescence. The number of population doublings till senescence was similar for cells cultured in FBS, but varied greatly for hMSCs cultured in AS. FBS cells accumulated in S phase of cell cycle. This could not be explained by increased express...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

181

Heightened awareness of a researcher's own culture through carrying out research on development cooperation

British Library Electronic Table of Contents (United Kingdom)

In this paper I argue that when a researcher is a research instrument, it is likely that the researcher would develop a heightened awareness of his/her own cultural conditioning, especially in the case of cross-cultural studies. And that such awareness would make him/her realise that one's own cultural background may indeed have an influence on formulating his/her research methodology. The paper is based on my own research project on development cooperation between Japan and Cambodia, whereby I examined the perceptions of Cambodians and Japanese on what an appropriate power relationship between donors and recipients should be. At the outset of my research project, I paid little attention to my own cultural condition and the role it might play in my study, though I was aware that being Japa...

2011-01-01

182

Explant culture of gastrointestinal tissue: a review of methods and applications

British Library Electronic Table of Contents (United Kingdom)

The gastrointestinal (GI) tract is an important target organ for the toxicity of xenobiotics. The toxic effects of xenobiotics on this complex, heterogeneous structure have been difficult to model in vitro and have traditionally been assessed in vivo. The explant culture of GI tissue offers an alternative approach. Historically, the organotypic culture of the GI tract proved far more challenging than the culture of other tissues, and it was not until the late 1960s that Browning and Trier described the means by which intestinal tissues could be successfully cultured. This breakthrough provided a tool researchers could utilise, and adapt, to investigate topics such as the pathogenesis of inflammatory intestinal diseases, the effect of growth factors and cytokines on intestinal proliferation...

2011-01-01

183

Chondrocytes and bone marrow-derived mesenchymal stem cells undergoing chondrogenesis in agarose hydrogels of solid and channelled architectures respond differentially to dynamic culture conditions

British Library Electronic Table of Contents (United Kingdom)

Abstract The objective of this study was to investigate how a combination of different scaffold architectures and rotational culture would influence the functional properties of thick cartilaginous tissues engineered using either chondrocytes or bone marrow-derived mesenchymal stem cells (BM-MSCs). Expanded porcine chondrocytes and BM-MSCs were suspended in 2% agarose and cast in custom-designed moulds to produce either regular solid or channelled construct cylinders. The study consisted of three seperate experimental arms. First, chondrocyte and BM-MSC constructs were cultured in free swelling conditions for 9 weeks. Second, constructs were subjected to rotational culture for a period of 3 weeks. Finally, BM-MSC-seeded constructs were subjected to delayed rotational culture, in which cons...

2011-01-01

184

Characteristics of enriched cultures for bio-huff-`n`-puff tests at Jilin oil field

Organ culture of mammalian skin and the effects of ultraviolet light and testosterone on melanocyte morphology and function

Three enriched cultures (48, 15a, and 26a), selected from more than 80 soil and water samples, could grow anaerobically in the presence of crude oil at 30{degrees}C and could ferment molasses to gases and organic acids. Oil recovery by culture 48 in the laboratory model experiment was enhanced by 25.2% over the original reserves and by 53.7% over the residual reserves. Enriched culture 48 was composed of at least 4 species belonging to the genera Eubacterium, Fusobacterium, and Bacteroides. This enriched culture was used as inoculum for MEOR field trials at Jilin oil field with satisfactory results. The importance of the role of these isolates in EOR was confirmed by their presence and behavior in the fluids produced from the microbiologically treated reservoir.

1995-12-31

185

Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of Calophyllum inophyllum L.

Scrotal skin of black Long-Evans rats and human thigh skin were maintained in vitro as organ cultures for as long as 14 days, and examined histologically using the combined skin splitting and Dopa techniques. Selected rat skin cultures received testosterone in the culture medium and/or were irradiated with ultraviolet light (290 to 320 nm uvl). With increased time in culture, scrotal melanocytes round up and there is an increase in epidermal pigmentation. Human skin behaves similarly; after eight days in vitro human melanocytes also become rounded, but remain strongly Dopa-positive. Addition of exogenous testosterone to cultured rat skin maintains dendritic morphology of melanocytes, but cell body size is still reduced. uvl irradiation stimulates melanocytes in rat skin cultures, maintaining their dendritic morphology and increasing epidermal and dermal ...

1978-05-01

186

British Library Electronic Table of Contents (United Kingdom)

Cell suspension cultures were initiated separately from leaf and nodal/internodal calluses for the study of influence of hormones and medium components on biomass growth and expression of dipyranocoumarins. Highest 6.2 times biomass was enhanced in suspension cultures of nodal/internodal callus supplemented with threefold total sulphate. Picloram 8.28mM along with BAP 8.88mM enhanced 295.05 times inophyllum A in suspension cultures of leaf callus whereas IBA 14.70mM along with BAP 4.44mM in suspension cultures of leaf callus enhanced 1065 times inophyllum B. IBA 4.90mM alone in suspension cultures of nodal/internodal callus enhanced maximum 616 times inophyllum C. Only IBA 9.80mM in suspension cultures of leaf callus enhanced 23.22 times inophyllum P. Variation in nitrate and sulphate had ...

2009-01-01

187

A structured approach to the assessment of the quality culture in nuclear installation

R-matrices for highest weight representations of s-circumflexl{sub q}(2,C) at roots of unity

INSAG has emphasized that safety culture has two general components: the organizational framework and the attitude of the staff. To develop a structured approach to the assessment of safety culture, we propose that the highly formalized nature of nuclear power plant organizations be exploited. The prime coordinating mechanism of NPP organizations is the standardization of work processes, where a work process is defined as a standardized sequence of tasks designed to achieve a specific goal (an example is the maintenance work process). The predictable nature of work processes is exploited by the Work Process Analysis Model (WPAM) to conduct a systematic analysis that identifies the desirable characteristics of work processes and develops performance measures for their strengths and weaknesses. These can provide a set of tangible characteristics of a good safety culture. It is argued in this paper that the analysis of normal ...

1995-04-01

188

Evaluation of antiasthmatic activity of Clitoria ternatea L. roots

The general formula is obtained for R-matrices of s-circumflexl{sub q}(2,C) for the highest weight representations both for general q and for q being a root of unity by generalizing G. Gomez`s and Sierra`s one for semiperiodic representations of s-circumflexl{sub q}(2,C) at roots of unity. In a case of general q, direct matrix form is obtained for the well known Jimbo`s R-matrix. In a case of q{sup N}=1 for semiperiodic and spin j<(N-1)/2 representations the new R-matrices are obtained which together with Jimbo`s one obey the spectral parameter dependent Yang-Baxter equations. (author) 16 refs.

1992-10-01

189

British Library Electronic Table of Contents (United Kingdom)

Aim of study: Clitoria ternatea L. (Family: Fabaceae) is being used in traditional medicine for the treatment of severe bronchitis and asthma. So the aim of study was to evaluate antiasthmatic activity of ethanol extract of Clitoria ternatea roots. Materials and methods: In the present study ethanol extract of Clitoria ternatea root (ECTR) was evaluated for preliminary phytochemical screening, acute toxicity studies and antiasthmatic activity using milk induced leucocytosis and eosinophilia in mice, egg albumin induced mast cell degranulations in rats and passive cutaneous anaphylaxis in rats at doses (100-150mg/kg ip). Results: The results of present investigation showed that the LD50 of ECTR is more than 1300mg/kg. ECTR significantly decreases milk induced leucocytosis and eosinophilia, ...

2011-01-01

190

Discrimination of Korean ginseng (Panax ginseng) roots using rapid resolution LC-QTOF/MS combined by multivariate statistical analysis

British Library Electronic Table of Contents (United Kingdom)

Korean ginseng (Panax ginseng C.A. Meyer) contains several types of saponins and ginsenosides, which are usually considered the major active components of ginseng. The types and quantities of saponins found in ginseng may differ depending on the region of cultivation. As a result, ginsengs produced in different areas of Korea have been unintentionally mislabeled and/or confused by Korean herbal markets owing to their complicated plant sources. Another concern is that 4- and 6-year-old ginseng roots, traditionally prescribed for different medicinal purposes, can vary in total saponin contents. Therefore, it is necessary to establish a reliable method for distinguishing different cultivation regions and ages of ginseng roots. A rapid resolution liquid chromatography-quadruple time of flight ...

2011-01-01

191

Differences in morphology, gas exchange and root hydraulic conductance before planting in Pinus canariensis seedlings growing under different fertilization and light regimes

British Library Electronic Table of Contents (United Kingdom)

As the main forestry species in the Canary Islands (Spain), Pinus canariensis is frequently used in afforestation programs. Several nursery techniques are commonly employed to modify its morphology and physiology with the aim of improving post-planting survival and growth. In this work, we studied how fertilization and light regime treatments applied during the nursery period modify biomass allocation patterns and produce effects in gas exchange and root hydraulic conductance. Seedlings were grown for a 6-month period in the nursery under two light regimes (full sunlight and 40% PAR reduction), and three fertilization levels were applied in each light regime. Morphology, biomass allocation patterns, leaf gas exchange and hydraulic conductance of the whole root system were evaluated. Fertil...

2010-01-01

192

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells in irradiated bone marrow cell culture in vitro

Context based inferences in research methodology: the role of culture in justifying knowledge claims

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells after irradiation were studied in the long-term culture of mouse bone marrow cells in vitro. No difference was observed in the survival of the stem cells among cultures in which 0 - 10/sup 7/ cells were re-inoculated on the adherent cell colonies in the culture flask. Stem cells showed a significant proliferation within 1 week and the number of the stem cells exceeded the control in 3 weeks after irradiation in the cultures with less than 10/sup 6/ re-inoculated cells per flask. In contrast, there was a considerable delay in the onset of stem cell proliferation after irradiation in the culture with 10/sup 7/ cells per flask. Based on these results, a possibility that a stimulator of stem cell proliferation, released from irradiated stromal cells, is cancelled by an inhibitory factor produced ...

1981-12-01

193

British Library Electronic Table of Contents (United Kingdom)

Drawing on work in epistemology and the philosophy of science, this paper seeks to provide very general reasons for why a comparative perspective needs to be applied to the inferential procedures of research methodologies where these concern the issue of justifying knowledge claims. In particular, the paper explores the role of culture on a number of important patterns of reasoning that figure in inferential arguments in research methodologies. The patterns examined are induction, both enumerative and analytical, hypothetico-deductive reasoning, and abductive inference. In each case it is argued that substantive theories about the world, including cultures, significantly affect inferential procedures. Examples chosen to illustrate this in more detail mostly reflect the impact of Confucian ...

2011-01-01

194

Cell cultures are more sensitive than Saccharamoyces cervisiae tests for assessing the toxicity of aquatic pollutants

The protein encoded by the rolB plant oncogene hydrolyses indole glucosides.

Cultured fish and human cells have been used as bioassay systems for the evaluation of the toxicity of aquatic pollutants. Numerous assays using bacteria and yeast have also been used for such purposes. The authors report the toxicity of aquatic pollutants (Cd, Hg, and Ni), using cell culture systems and the yeast Saccharomyces cervisiae test. Cd, Hg, and Ni were chosen as model compounds of pollutants because the related toxicity is now fairly well established.

1988-07-01

195

Synthesis, Salvage, and Catabolism of Uridine Nucleotides in Boron-Deficient Squash Roots ¹

The rolB gene of Agrobacterium rhizogenes, whose expression stimulates the formation of roots by transformed plant tissues and other growth alterations in transgenic plants, codes for a beta-glucosidase...Full Text Available

1991-11-01

196

Succession of Indigenous Pseudomonas spp. and Actinomycetes on Barley Roots Affected by the Antagonistic Strain Pseudomonas fluorescens DR54 and the Fungicide Imazalil

Previous work has provided evidence that plants may require boron to maintain adequate levels of pyrimidine nucleotides, suggesting that the state of boron deficiency may actually be one of pyrimidine...Full Text Available

1981-12-01

197

Seasonal Patterns of Nitrate Reductase and Nitrogenase Activities in Phaseolus vulgaris L. ¹

In recent years, the interest in the use of bacteria for biological control of plant-pathogenic fungi has increased. We studied the possible side effects of coating barley seeds with the antagonistic...Full Text Available

2001-03-01

198

Role of copper resistance in competitive survival of Pseudomonas fluorescens in soil.

The patterns of nitrate reductase activity (NRA) in the leaves (in vivo assay) and root nodule nitrogenase activity (C₂H₂ reduction) were investigated throughout...Full Text Available

1979-03-01

199

Role of Respiratory Nitrate Reductase in Ability of Pseudomonas fluorescens YT101 To Colonize the Rhizosphere of Maize

A copper-resistant strain (09906) of Pseudomonas fluorescens that was isolated from a citrus grove soil is being investigated as a biological control agent for Phytophthora root rot. Since citrus grove...Full Text Available

1993-02-01

200

Role of Military Workingn> Dogs in Low Intensity Conflict.

Selection of the denitrifying community by plant roots (i.e., increase in the denitrifier/total heterotroph ratio in the rhizosphere) has been reported by several authors. However, very few studies...Full Text Available

2000-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

201

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

This paper examines the current military working dogs (MWD) program, its historical roots, and the possibility for expansion of the program. Current service MWD programs will be discussed, as well as possible models for change. The primary focus is on the...

1990-01-01

202

Radioactivity of Tobacco Leaves and Radiation Dose Induced from Smoking

Carbenoxolone (CBX) is a semisynthetic derivative of the licorice root substance glycyrrhizinic acid and has been previously reported to induce only heat shock protein 70 [Hsp70, HSPA1A (the systematic...Full Text Available

2009-09-01

203

Phytochrome Control of Another Phytochrome-mediated Process ¹

The radioactivity in tobacco leaves collected from 15 different regions of Greece and before cigarette production was studied in order to find out any association between the root uptake of radionuclides...Full Text Available

2009-02-01

204

Patch Clamp Studies on Root Cell Vacuoles of a Salt-Tolerant and a Salt-Sensitive Plantago Species ¹

The phytochrome-mediated attachment of root tips of mung bean (Phaseolus aureus) and barley (Hordeum vulgare) to glass is affected by the prior exposure of hydrated...Full Text Available

1972-04-01

205

Molecular Characterization of Aluminium (aluminum) Tolerance in Rye

Plantago media L. and Plantago maritima L. differ in their strategy toward salt stress, a major difference being the uptake and distribution of ions. Patch clamp techniques...Full Text Available

1990-01-01

206

Localization and Toxic Effects of Cadmium, Copper, and Uranium in Azolla¹

Aluminium (Al) toxicity, affecting around half of the world¿s arable land, severely hinders the ability of crop plants to utilize moisture and nutrients by restricting root growth and function. Among the cultivated cereals, rye is the most Al-tolerant and represents an important potential source of ...

207

Left Main Coronary Stenosis as a Consequence of Bentall Operation: Percutaneous Treatment

The storage and distribution of copper, cadmium, and uranium and their effects on ionic contents in roots and shoots of Azolla filiculoides has been studied by x-ray microanalysis....Full Text Available

1988-09-01

208

Kinetics of the Vacuolar H⁺-Pyrophosphatase ¹

A 65-year-old man suffering from ascending aorta aneurysm and atherosclerotic three vessel disease without left main involvement underwent aortic root replacement with coronary ostia reimplantation...Full Text Available

2009-01-01

209

Isolation and identification of flavonoid (-)-4'-methyl-epigallocatechin of roots from Maytemus obtusifolia Mart. (Celastraceae)

The responses of the vacuolar membrane (tonoplast) proton-pumping inorganic pyrophosphatase (H⁺-PPase) from oat (Avena sativa L.) roots to changes in Mg²⁺ and...Full Text Available

1992-12-01

210

Indoleacetic Acid and Abscisic Acid Antagonism

Portuguese ... Orig. Title Isolamento e identificacao do flavonoide (-)-4'-metil-epigalocatequina das raizes de Maytenus obtusifolia Mart. (Celastraceae)

2006-05-19

211

Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an Avena sativa Plasma Membrane Determinant 1

The phytochrome-mediated attachment of mung bean (Phaseolus vulgaris L., var. Oklahoma 612) root tips on glass is quickly affected by indoleactic acid and abscisic acid at concentrations...Full Text Available

1973-01-01

212

High genetic variability and low local diversity in a population of arbuscular mycorrhizal fungi

An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target...Full Text Available

1992-01-01

213

Expression of leukotriene receptors in the rat dorsal root ganglion and the effects on pain behaviors

Arbuscular mycorrhizal fungi (AMF) are ecologically important root symbionts of most terrestrial plants. Ecological studies of AMF have concentrated on differences between species; largely assuming...Full Text Available

2004-02-24

214

Evidence for the Regulation of Phytochrome-mediated Processes in Bean Roots by the Neurohumor, Acetylcholine ¹

BackgroundLeukotrienes (LTs) belong to the large family of lipid mediators implicated in various inflammatory conditions such as asthma and rheumatoid arthritis. Four distinct types...Full Text Available

215

Effects of Multivalent Cations on Cell Wall-Associated Acid Phosphatase Activity

Using pharmacological and chromatographic techniques, it was shown that acetylcholine was present in all organs of both light- and dark-grown mung bean seedings (Phaseolus aureus)....Full Text Available

1970-12-01

216

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity

Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the...Full Text Available

1988-09-01

217

Cholinesterases from Plant Tissues

Cytokinins are hormones that regulate cell division and development. As a result of a lack of specific mutants and biochemical tools, it has not been possible to study the consequences of cytokinin...Full Text Available

2003-11-01

218

Carotenoid Crystal Formation in Arabidopsis and Carrot Roots Caused by Increased Phytoene Synthase Protein Levels

2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride (AMO-1618) inhibits the activity of a cholinesterase isolated from mung bean (Phaseolus aureus) roots...Full Text Available

1973-09-01

219

CNS-derived glia ensheath peripheral nerves and mediate motor root development

BackgroundAs the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches...Full Text Available

220

Boron Tolerance in Barley Is Mediated by Efflux of Boron from the Roots1

Motor function requires that motor axons extend from the spinal cord at regular intervals and that they are myelinated by Schwann cells. Little attention has been given to another cellular structure,...Full Text Available

2008-02-01

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221

Biosynthesis, Translocation, and Accumulation of Betaine in Sugar Beet and Its Progenitors in Relation to Salinity 12

Many plants are known to reduce the toxic effects of high soil boron (B) by reducing uptake of B, but no mechanism for limiting uptake has previously been identified. The B-tolerant cultivar of barley...Full Text Available

2004-10-01

222

Anti-Inflammatory and Immunomodulatory Effects of Paeonia Lactiflora Pall., a Traditional Chinese Herbal Medicine

Like other halophytic chenopods, sugar beet (Beta vulgaris L.) can accumulate high betaine levels in shoots and roots. N,N,N-trimethylglycine impedes sucrose crystallization...Full Text Available

1982-10-01

223

Activity-Local Symbolic State Graph Generation for High-Level ...

In China, Korea, and Japan, a decoction of the dried root without bark of Paeonia lactiflora Pall. has been used in the treatment of rheumatoid arthritis, systemic lupus erythematosus,...Full Text Available

224

A Model for Predicting Ionic Equilibrium Concentrations in Cell Walls ¹

... ie on all paths from the root to a terminal node we ... (A) A stochastic Petri net ... Tandem Queueing Network [HMKS99] 63 26 19 173 2.297e4 7.148e ...

2005-09-01

225

To talk or not to talk: exploring culturally diverse patients' health information communication choices.

Purified cell walls were prepared from roots of Horse bean (Vicia faba L., var. minor) and Yellow Lupine (Lupinus luteus L.). Two methods were used:...Full Text Available

1981-08-01

226

The actin content of fibroblasts.

As care shifts from institutional to home- and community-based settings, consumer health information technology (IT) must be designed to support patients' new health information management responsibilities. We developed and piloted a new methodology grounded in social network analysis and human factors engineering to explore two often overlooked aspects of this phenomenon: the task of health information communication with members of the social network and the context of culture. Such knowledge is necessary to inform the appropriate design of consumer health IT. We asked a culturally diverse sample of participants to describe what, to whom, why, and how they communicate health information and to provide direct feedback about the methodology. The methodology was acceptable to all participants and able to capture similarities and differences in their health information communication practices. Prior to the main study we will need to refine the ...

2010-11-13

227

The Structure of Plant Cell Walls

Cultures of chick skin fibroblasts were dissolved in solutions of sodium dodecyl sulphate, and their entire protein content was examined by gel electrophoresis. The most abundant species migrated in...Full Text Available

1975-05-01

228

The I3I Model; Identifying Cultural Determinants of Information ...

The molecular structure, chemical properties, and biological function of the xyloglucan polysaccharide isolated from cell walls of suspension-cultured sycamore (Acer pseudoplatanus)...Full Text Available

1973-01-01

229

Production of Amylase in Liquid Culture by a Strain of Aspergillus oryzae

... Information behavior in dynamic group work contexts: Interwoven situational awareness, dense social networks and contested collaboration in ...

2009-06-01

230

Novel Cytotoxic Vectors Based on Adeno-Associated Virus

The effect of different media and pH on the formation of amylase by Aspergillus oryzae EI 212 is described. Depending upon the composition of the medium and growth...Full Text Available

1970-04-01

231

Wastenet

positive primary PymT breast cancer cells in primary co-cultured tumor tissue, suggesting target specificity of

232

Nineteen Cases of Plague in Arizona

Metamorphosis of Confucian Heritage Culture and the possibility of an Asian education research methodology

We review the cases of 19 successfully treated plague patients, with emphasis on the clinical and epidemiologic features of the disease. Proper staining and culturing of bubo aspirates; prompt institution...Full Text Available

1985-05-01

233

British Library Electronic Table of Contents (United Kingdom)

This paper opens with a critical analysis of a paradox in contemporary educational research in and about Confucian Heritage Culture (CHC): the assumption that national boundaries coincide with those of a distinct and homogeneous culture, which consistently renders a rather homogenous set of educational phenomena, and collides against a more widely accepted discourse - culture transcends geographical frontiers and is ever evolving in character. It is claimed that this paradox is due to the fact that a thin conception of CHC competes neck-and-neck with a thick conception of it. This paper also addresses the possibility of an ad hoc education research methodology in and about CHC and its compliance issues regarding the mainstream Western research dynamics and philosophy of science. Confucian ...

2011-01-01

234

Invasive Species: State Resources - Colorado

Hispanic Cultural Influences on Medical Practice

of Concern; Parks; Management; Monitoring Exotic and Invasive Species Northern Arizona University. Canyons, Cultures, and Environmental Change. Species of Concern; Contacts;...

2011-10-01

235

Because the proportion of Hispanic patients is increasing rapidly, most physicians are now coming into daily contact with Hispanics. In addition to obvious difficulty with oral communication, Hispanics...Full Text Available

1983-10-01

236

Filopodia

Factors Influencing the Effectiveness of Swimming Pool Bactericides

The processes of neuronal outgrowth and guidance have typically been studied in classic 2D cell culture systems that do not recapitulate topographical cues present in the in vivo extracellular matrix...Full Text Available

2011-07-01

237

Experience of isolated sleep paralysis in clinical practice in Nigeria.

Techniques for culturing, harvesting, and testing bacteria to evaluate bactericidal chemicals for swimming pools are described. Concentrations of 25, 50, and 100 mg of the chlorine stabilizer cyanuric...Full Text Available

1967-05-01

238

Evaluation of the microbiology of chronic ethmoid sinusitis.

The supernatural fears associated with the experience of isolated sleep paralysis in the culture of developing countries is sometimes associated with the evolution of somatic symptoms of psychological...Full Text Available

1992-06-01

239

Eukaryotic Diversity in an Anaerobic Aquifer Polluted with Landfill Leachate?

In a prospective study, patients with the diagnosis of chronic ethmoid sinusitis were evaluated microbiologically by using biopsy specimens of the ethmoid sinus mucosa. Microbiology cultures were performed...Full Text Available

1991-11-01

240

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and...Full Text Available

2008-07-01

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241

British Library Electronic Table of Contents (United Kingdom)

Abstract The glycosylation profile of therapeutic antibodies is routinely analyzed throughout development to monitor the impact of process parameters and to ensure consistency, efficacy, and safety for clinical and commercial batches of therapeutic products. In this study, unusually high levels of the mannose-5 (Man5) glycoform were observed during the early development of a therapeutic antibody produced from a Chinese hamster ovary (CHO) cell line, model cell line A. Follow up studies indicated that the antibody Man5 level was increased throughout the course of cell culture production as a result of increasing cell culture medium osmolality levels and extending culture duration. With model cell line A, Man5 glycosylation increased more than twofold from 12% to 28% in the fed-batch process...

2011-01-01

242

Effect of Cytokines and bFGF on the Osteoclast Differentiation Induced by $1\\;{\\alpha},25-(OH)_2D_3$ in Primary Murine Bone Marrow Cultures

Development and Reproduction of grape phylloxera on irradiated in vitro cultured rootstocks

Full Text Available

1999-12-01

243

Detection and Identification of Bacteria by Gas Chromatography¹

The ability of a local strain of grape phylloxera to develop and reproduce on irradiated in vitro cultured rootstocks (Ru140, R99 and 3309C and one local variety 'Helwani' was determined. The results showed that there was great variation in developmental time and reproduction of phylloxera between irradiated and unirradiated in vitro cultured plants. Survival, number of eggs and mean developmental time were greatly reduced when phylloxera was reared on irradiated rootstocks. Based on the examined biological parameters of phylloxera, all tested rootstocks would be more resistant toward such destructive insect when they were irradiated. Thus, phylloxera resistance was enhanced when in vitro cultured plants were treated with low doses of gamma irradiation. (author)

244

Cultures of Death and Politics of Corpse Supply

Ether extracts of cultures of 29 strains representing 6 species of Bacillus, and of individual strains of Escherichia coli, Aerobacter aerogenes, and Pseudomonas...Full Text Available

1966-07-01

245

Context, Culture, and Connection: Avoiding the Counter ...

SummaryNineteenth-century Vienna is well known to medical historians as a leading centre of medical research and education, offering easy access to patients and corpses to students...Full Text Available

2008-01-01

246

Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces

... Page 25. US Department of Defense, Joint Operation Planning and Execution System, Vol 1, Planning Policies and Procedures (CJCSM 3122.01A). ...

2008-04-23

247

Association between the number of blood cultures and appropriateness of care for suspected bacteremic urinary tract infection in the elderly

BackgroundOsseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility...Full Text Available

248

British Library Electronic Table of Contents (United Kingdom)

The objective of this study was to evaluate the association between the number of blood cultures collected and the appropriateness of care for suspected bacteremic community-acquired urinary tract infection (UTI) in the elderly. We retrospectively evaluated the medical records of 129 patients with UTI >65?years old admitted to a large community-based training hospital in Japan from 1 January 2006 to 31 December 2009. We assessed the association between the number of blood cultures collected and the appropriateness of care received, as well as other factors. Two-thirds of the patients were women, and patients >85?years old accounted for 45.0% of the cases. Most of the organisms isolated from the urine and blood were Escherichia coli (65.4?67.0%). More than two blood cultures were collected ...

2011-01-01

249

Apoptosis at Inflection Point in Liquid Culture of Budding Yeasts

Accumulation, Activity and Localization of Cell Cycle Regulatory Proteins and the Chloroplast Division Protein FtsZ in the Alga Scenedesmus quadricauda under Inhibition of Nuclear DNA Replication

Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known ...Full Text Available

250

British Library Electronic Table of Contents (United Kingdom)

Synchronized cultures of the green alga Scenedesmus quadricauda were grown in the absence (untreated cultures) or in the presence (FdUrd-treated cultures) of 5-fluorodeoxyuridine, the specific inhibitor of nuclear DNA replication. The attainment of commitment points, at which the cells become committed to nuclear DNA replication, mitosis and cellular division, and the course of committed processes themselves were determined for cell cycle characterization. FdUrd-treated cultures showed nearly unaffected growth and attainment of the commitment points, while DNA replication(s), nuclear division(s) and protoplast fission(s) were blocked. Interestingly, the FdUrd-treated cells possessed a very high mitotic histone H1 kinase activity in the absence of any nuclear division(s). Compared with the ...

2008-01-01

251

A Preliminary Study on Dressing Patterns and Incidence of Candidiasis

A Cultural Resources Survey Testing, and Geomorphic ...

The incidence of candidiasis in two groups of voluntary participants wearing tight and loose fitted dresses was investigated by both microscopic and cultural techniques for a period of two months....Full Text Available

1982-02-01

252

Why is the null HBT result at RHIC so interesting?

... Aerial photographs were provided by Mr. Grover P. ... Dr. Beverely J. Watkins conducted the records search and wrote the historic research. x,/ ...

1987-04-01

253

The root cause analysis of 9DVN002ZV fan failure in Daya Bay Nuclear Power Station

Pion interferometry (HBT of A+A) data have posed a thorn in the theoretical interpretation of AA collisions at RHIC (#sq root#s = 130 AGeV). How can R_o_u_t #approx# R_s_i_d_e #approx# R_l_o_n_g and remain so between AGS and RHIC? Where is the QGP Stall? Can elephants hide along the x_0"+ dimension? We rummage old hydrodynamic scenarios and uncover some previously ignored NULL solutions. (author)

254

Spin-orbit splittings in the relativistic mean-field theory

Extensive investigations and detailed analysis of the failure reason of 9DVN002ZV fan in Daya Bay Nuclear Power Station showed that the fan destroy was caused by the failure of non-drive end bear. The direct cause of this bearing' failure was its improper assembly caused by improper maintenance procedure, and the root cause was too small internal radial clearance after mounting. The factor affecting bearing internal radial clearance, the relationship between clearance and operating life time and fan failure process were discussed. (authors)

2005-09-01

255

Nutrient regulation of the saprotroph to parasite transition in Pochonia chlamydosporia, a soil microbial inoculant for nematode control

We have investigated the effect of the rho tensor coupling on binding energies, matter root-mean-square radii and spin orbit splittings of Ca isotopes in the relativistic mean-field theory with sigma, omega, and rho mesons. It is shown that binding energies and matter root-mean-square radii are insensitive to an alteration in the strength of the rho tensor coupling and an explanation of this is given. We have further shown that inclusion of the rho tensor coupling will give isospin-dependent spin-orbit splittings and this will greatly affect spin-orbit splittings of nuclei near the neutron drip line. (author). Letter-to-the-editor.

1995-11-01

256

Environmental Research Database

DescriptionRoot-knot nematodes (Meloidogyne spp.) are major nematode pests of most tropical crops, making roots less efficient at withdrawing nutrients and water from soil, sometimes causing the total failure of crops grown by resource-poor farmers in Africa. Nematicides are some of the most toxic products used in crop protection, and are inappropriate or too expensive for use on most crops in Africa and there is an urgent need for new methods of nematode management. The fungus Pochonia chlamydosporia is [continued...

2011-01-31

257

Antimicrobial potential of Glycyrrhiza glabra roots

British Library Electronic Table of Contents (United Kingdom)

The present study was aimed to investigate antimicrobial potential of Glycyrrhiza glabra roots. Antimycobacterial activity of Glycyrrhiza glabra was found at 500mg/mL concentration. Bioactivity guided phytochemical analysis identified glabridin as potentially active against both Mycobacterium tuberculosis H37Ra and H37Rv strains at 29.16mg/mL concentration. It exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. Our results indicate potential use of licorice as antitubercular agent through systemic experiments and sophisticated anti-TB assay.

2008-01-01

258

Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root.

The Isolation and Characterization of d-Glucose 6-Phosphate Cycloaldolase (NAD-Dependent) from Acer pseudoplatanus L. Cell Cultures

Glycyrrhiza glabra root is one of the common traditional Chinese medicines and used as flavoring and sweetening agents for tobaccos, chewing gums, candies, toothpaste and beverages. While glycyrrhizin is one of the main components in the extract of G. glabra root and has been characterized, the other components have not been well characterized. The mechanism of growth activation of breast cancer MCF-7 cells, including the activation of Erk1/2 and Akt, and the transcriptional regulation of estrogen-responsive genes, was examined by means of sulforhodamine B, luciferase reporter gene, real-time RT-PCR and Western blotting assays after the induction of the cells with the extract of G. glabra root. The extract has similar activity to that induced by 17beta-estradiol (E(2)), although glycyrrhizin did not show such an activity. Moreover, the estrogen receptor alpha-dependent neurite outgrowth induced by the extract was similar to ...

2007-06-24

259

Regulation of Indole-3-Acetic Acid Biosynthetic Pathways in Carrot Cell Cultures ¹

A soluble enzyme system from suspension cultures of Acer pseudoplatanus L. converts d-glucose 6-phosphate to myoinositol. A Mg²⁺-dependent phosphatase, present in...Full Text Available

1971-09-01

260

Plant cell engineering: current research, application and future prospects

2,4-Dichlorophenoxyacetic acid (2,4-D) promotes the accumulation of tryptophan-derived indole-3-acetic acid (IAA) in carrot cell cultures during callus proliferation by a biosynthetic pathway that is...Full Text Available

1992-11-01

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261

Inhibitory effect of transforming growth factor-. beta. (TGF-. beta. ) on insulin-like growth factor 1 (IGF-1)-induced proliferation and differentiation in primary cultures of pig preadipocytes

This paper reviewed the current status of basic research in plant cell engineering, highlighted the application of embryo culture, double haploid (DH) technology, protoplast culture and somatic hybridization, somaclonal variation, rapid propagation, and bio-products production of plant-origin, and t he prospects. (authors)

2008-10-01

262

Inhibitory effect of transforming growth factor-#beta# (TGF-#beta#) on insulin-like growth factor 1 (IGF-1)-induced proliferation and differentiation in primary cultures of pig preadipocytes

The influence of serum, IGF-1 and TGF-{beta} on the differentiation of preadipocytes was examined in primary cultures of porcine adipose tissue cells. In serum-supplemented or serum-free, IGF-1 (1 and 10 nM) had no effect on total cell number. However, IGF-1 (10nM) increased adipocyte number only in serum-supplemented (1% pig serum) cultures, whereas TGF-{beta} (15 pm) reduced the adipocyte number in the presence and absence of IGF-1. Replication of preadipocytes was analyzed with a ({sup 3}H) thymidine assay. Preadipocyte proliferation (cpm in adipocyte fraction) was increased by IGF-1 (10nM) only in cultures containing pig serum. TGF-{beta} had no effect on preadipocyte proliferation specifically, but slightly increased total ({sup 3}H) thymidine incorporation in cultures with serum. Glycerol phosphate dehydrogenase (GPDH) specific activity was decreased by adding TGF-{beta} to serum-free ...

1990-02-26

263

Evaluation of the Bactec MGIT 960 system in combination with the MGIT TBc identification test for detection of Mycobacterium tuberculosis complex in respiratory specimens.

The influence of serum, IGF-1 and TGF-#beta# on the differentiation of preadipocytes was examined in primary cultures of porcine adipose tissue cells. In serum-supplemented or serum-free, IGF-1 (1 and 10 nM) had no effect on total cell number. However, IGF-1 (10nM) increased adipocyte number only in serum-supplemented (1% pig serum) cultures, whereas TGF-#beta# (15 pm) reduced the adipocyte number in the presence and absence of IGF-1. Replication of preadipocytes was analyzed with a ["3H] thymidine assay. Preadipocyte proliferation (cpm in adipocyte fraction) was increased by IGF-1 (10nM) only in cultures containing pig serum. TGF-#beta# had no effect on preadipocyte proliferation specifically, but slightly increased total ["3H] thymidine incorporation in cultures with serum. Glycerol phosphate dehydrogenase (GPDH) specific activity was decreased by adding TGF-#beta# to serum-free ...

1991-04-21

264

Effect of Elicitation and Changes in Extracellular pH on the Cytoplasmic and Vacuolar pH of Suspension-Cultured Soybean Cells 1

The sensitivity and specificity of the MGIT TBc identification (TBc ID) test for Mycobacterium tuberculosis complex (MTC) detection in positive Bactec MGIT cultures were 95.2% and 99.2%, respectively. When MTC-positive results obtained from two additional molecular methods were included, the sensitivity of the MGIT TBc ID test was 85.4%, while that of culture was 95.7%. PMID:21450949

2011-03-30

265

Control mechanisms operating for lipid biosynthesis differ in oil-palm (Elaeis guineensis Jacq.) and olive (Olea europaea L.) callus cultures.

We have employed both ³¹P nuclear magnetic resonance spectroscopy and two intracellular fluorescent pH indicator dyes to monitor the pH of the vacuole and cytoplasm of suspension-cultured...Full Text Available

1992-02-01

266

Soil less culture; I sistemi di coltivazione senza suolo

As a prelude to detailed flux control analysis of lipid synthesis in plants, we have examined the latter in tissue cultures from two important oil crops, olive (Olea europaea L.) and oil palm (Elaeis...Full Text Available

2002-06-01

267

Identification of culturable and originally non-culturable endophytic bacteria isolated from shoot tip cultures of banana cv. Grand Naine

The paper gives a general view of techniques and systems related to soil less culture developed in the last years (on substrate in beg; NFT; Ebb-Flood, aeroponic,..) taking into account their management and problems (water quality, control of plant nutrition and irrigation; substrates; pathological aspects,..). The evolution, now in progress, of soil less culture from open to closed system as a way to realized an environmental friendly growing system, is considered. When plants are grown with open cycle techniques a large amount of waste solution, with an a high content of nutrients, are discharged in soil and water. Furthermore, they need an extra-utilization of water and fertilizers. Another aspect is the utilization of low cost substrates, which can be reused for more than one cultural cycle without negative effects on yield, and also finally discharged without negative effects on the environment. The development of soil ...

1996-01-01

268

British Library Electronic Table of Contents (United Kingdom)

In this article we describe the identification of endophytic bacteria belonging to three groups isolated from shoot tip cultures of banana cv. Grand Naine in a recent study (Thomas et?al. 2008) based on partial 16S rRNA gene sequence homology analysis. The first group included banana stocks that displayed obvious colony growth on MS based tissue culture medium during the first in?vitro passage. The second group constituted stocks that were tissue index-negative for cultivable bacteria initially but turned index-positive after a few to several (4?8) in?vitro passages while the third group formed one sub-stock that turned index-positive after about 18 passages. The organisms belonged to about 20 different genera comprising of ?, ?, ?-proteobacteria, Gram-positive firmicutes and actinobacteri...

2008-01-01

269

Atrial natriuretic peptide receptor heterogeneity and effects on cyclic GMP accumulation

Fluxes of H+ and K+ in corn roots

The effects of atrial natriuretic peptide (ANP), oxytocin (OT) and vasopressin (AVP) on guanylate cyclase activity and cyclic GMP accumulation were examined, since these hormones appear to be intimately associated with blood pressure and intravascular volume homeostasis. ANP was found to increase cyclic GMP accumulation in ten cell culture systems, which were derived from blood vessels, adrenal cortex, kidney, lung, testes and mammary gland. ANP receptors were characterized in intact cultured cells using {sup 125}I-ANP{sub 8-33}. Specific {sup 125}I-ANP binding was saturable and of high affinity. Scratchard analysis of the binding data for all cell types exhibited a straight line, indicating that these cells possessed a single class of binding sites. Despite the presence of linear Scatchard plots, these studies demonstrated that cultured cells possess two functionally and physically distinct ANP-binding sites. Most of the ...

1988-01-01

270

The role of acid incubation in rapid immobilization of hydrogen-producing culture in anaerobic upflow column reactors

We report here on an experimental system that utilizes ion-selective microelectrodes to measure the electrochemical potential gradients for H"+ and K"+ ions within the unstirred layer near the root surface of both tact 4-day-old corn seedlings and corn root segments. Analysis of the steady state H"+ and K"+ electrochemical potential gradients provided a simultaneous measure of the fluxes crossing a localized region of the root surface. Net K"+ influx values obtained by this method were compared with unidirectional K"+ ("8"6Rb"+) influx kinetic data; at any particular K"+ concentration, similar values were obtained by either technique. The ion-specific microelectrode system was then used to investigate the association between net H"+ efflux and net K"+ influx. Although the computed H"+K"+ stoichiometry is dependent upon the choice of diffusion coefficients, the values obtained were extremely variable, and net K"+ influx ...

1987-01-01

271

Study on the use of coal ash reclaimed land as upland-fields (Part 2)

An approach of acidification was examined on formation of hydrogen-producing granules and biofilms in upflow column-shaped reactors. The reactors were fed with synthetic glucose wastewater and operated at 37 C and pH 5.5. The acclimated anaerobic culture was inoculated in four reactors designated R1, R2, R3 and R4, with R3 and R4 filled with granular activated carbon as support medium. To unveil the roles of acidification, microbial culture in R2 and R3 was subject to an acid incubation for 24 h by shifting the culture pH from 5.5 to 2.0. The experimental results suggested that the acidification substantially accelerated microbial granulation, but not biofilm formation. Microbial activities were inhibited by the acid incubation for about 78 h, resulting in the retarded formation of biofilms of the acidified culture. Reducing culture pH resulted in improvement in cell surface ...

2008-10-15

272

Dynamics of accumulation and disappearance of cobalt-60 in wheat-soil system

Soil dressing on coal ash reclaimed land is a covering to use the land for agriculture. This study was carried out to find out the necessary depth of soil covering the ash layer in order to have normal crop growth. Two kinds of crops, Japanese radish, and rakkyo were planted in cover soil on the fly ash packed in wooden boxe (90 cm x 90 cm x 90 cm). Depths of cover soil were 20 cm, 30 cm, 40 cm and 50 cm. The results were summarized as follows: growth and yield of Japanese radish and rakkyo were increased with increasing depth of cover soil; root development of Japanese radish was inhibited at the lower coal ash layer. Main root length and edible root length of Japanese radish were decreased with decreasing depth of cover soil; boron and molybdenum contents in the plants remarkably increased with decreasing depth of cover soil. This may be due to the absorption of these elements from fly ash layer; it is concluded that ...

1987-01-01

273

Compost amendment of Cu-Zn minespoil reduces toxic bioavailable heavy metal concentrations and promotes establishment and biomass production of Bromus carinatus (Hook and Arn.)

The dynamics of transportation, accumulation, disappearance and distribution of "6"0Co in the wheat-soil system was studied by using isotope-tracer techniques for simulated pollutants, and the mathematical model of the behavior was established. Rescilts showed that (1) "6"0Co was transported rapidly in the system after the application on soil surface layer. The uptake of "6"0Co by wheat was mainly via root, and redistribution in all parts of wheat occurred consequently. The concentration in root was much higher than that in other parts of wheat plant. The specific activity of "6"0Co in wheat plant rapidly increased to a maximum value, then decreased. The specific activity of "6"0Co in each part of wheat plant was found in the order of root > straw > husk > seed. (2) "6"0Co is mainly detained with in 6cm of soil surface, and specific activity of "6"0Co in soil present a simple exponential declining with depth of ...

2008-10-01

274

Assessing the Significance of Above- and Belowground Carbon Allocation of Fast- and Slow-Growing Families of Loblolly Pine - Final Report

A series of lab and greenhouse studies were undertaken to understand how Cu and Zn toxicity influences Bromus carinatus (Hook and Arn.) growth, to what degree an organic amendment (yard waste compost) may reduce Cu and Zn bioavailability in Cu-Zn minespoil and promote plant growth in combination with fertilizer, and how the vertical distribution of compost in the minespoil influences rooting depth. Root Cu and Zn toxicity thresholds were determined to be 1 mg L"-"1 and 10 mg L"-"1 in solution, respectively. The compost amendment had exceptionally high Cu and Zn binding capacities (0.17 and 0.08 g metal g C"-"1, for Cu and Zn, respectively) that were attributed to high compost humic and fulvic acid concentrations. Maximum plant biomass was achieved when minespoil was amended with compost and fertilizer in combination. Fertilizer alone had no effect on plant growth. Mixing compost into the minespoil was essential to promote adequate ...

2007-07-01

275

Studies on the appearance of skeletal anomalies in red porgy: effect of culture intensiveness, feeding habits and nutritional quality of live preys

During this project we experimentally evaluated the below-ground biomass and carbon allocation and partitioning of four different fast- and slow-growing families of loblolly pine located in Scotland County, NC, in an effort to increase the long-term performance of the crop. The trees were subjected to optimal nutrition and control since planting in 1993. Destructive harvests in 1998 and 2000 were used for whole?plant biomass estimates and to identify possible family differences in carbon acquisition (photosynthesis) and water use efficiency. At regular intervals throughout each year we sampled tissues for carbohydrate analyses to assess differences in whole-tree carbon storage. Mini rhizotron observation tubes were installed to monitor root system production and turnover. Stable isotope analysis was used to examine possible functional differences in water and nutrient acquisition of root systems between the various families. A genetic ...

2001-03-01

276

British Library Electronic Table of Contents (United Kingdom)

Summary Despite the great interest of red porgy as a new species for Mediterranean aquaculture, its commercial production is constrained by the high incidence of skeletal deformities occurring in this species under culture conditions. Several studies have been conducted to better understand the origin of these anomalies in this species, using different system intensiveness, rotifers enrichment products or rotifers docosahexaenoic acid content. The first study showed that culture intensification increased the number of fish with an extra vertebrae, what was probably related to the different nutritional quality of live preys employed in each treatment, since water temperature, salinity and genetic background were identical for the different batches of fish studied. Total incidence of skeleta...

2010-01-01

277

Social involvement and development as a response to the campus student culture

British Library Electronic Table of Contents (United Kingdom)

Given the widely accepted notion of whole person education in Confucian societies such as Hong Kong, Mainland China and Singapore, it is surprising that research literature originated in these societies pays little attention to how students learn and develop through out-of-class experiences at university. There is little research evidence on how the prevailing culture among student social communities (residential halls and student societies/clubs) influences students? social involvement and development. This paper examines 42 Chinese students? social experiences and development during their freshman year at a Hong Kong university. The majority of them were intensively involved in out-of-class activities. Their active social involvement was both a response to the culture of student communit...

2011-01-01

278

Rapamycin (sirolimus) protects against hypoxic damage in primary heart cultures via Na^+/Ca^2^+ exchanger activation

British Library Electronic Table of Contents (United Kingdom)

Aims: Rapamycin (sirolimus) is an antibiotic that inhibits protein synthesis through mammalian targeting of rapamycin (mTOR) signaling, and is used as an immunosuppressant in the treatment of organ rejection in transplant recipients. Rapamycin confers preconditioning-like protection against ischemic-reperfusion injury in isolated mouse heart cultures. Our aim was to further define the role of rapamycin in intracellular Ca^2^+ homeostasis and to investigate the mechanism by which rapamycin protects cardiomyocytes from hypoxic damage. Main methods: We demonstrate here that rapamycin protects rat heart cultures from hypoxic-reoxygenation (H/R) damage, as revealed by assays of lactate dehydrogenase (LDH) and creatine kinase (CK) leakage to the medium, by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-d...

2011-01-01

279

Politics, guanxi and the search for objectivity: the intricacies of conducting educational research in Chinese contexts

British Library Electronic Table of Contents (United Kingdom)

This article discusses how history, the cultural setting, and the political-ideological contexts may influence educational research in China. It seeks to demonstrate a dichotomy between official and popular discourses, and argues that there is a need for the researcher to understand and interpret the language style used in various interview settings and research publications in China. Further, it is contended that ideology and cultural influences push towards a 'virtuous' or socially acceptable understanding of reality. These ideological and cultural norms may also affect official research data and statistics. Further, the article seeks to demonstrate that for a researcher in China (whether Chinese or not) it is important to cultivate and make use of guanxi (connections), at the same time ...

2011-01-01

280

Patterns of proliferation and differentiation of irradiated haemopoietic stem cells cultured on normal 'stromal' cell colonies in vitro

Environmental, scanning electron and optical microscope image analysis software for determining volume and occupied area of solid-state fermentation fungal cultures

Experiments were designed to elucidate whether or not the irradiated bone marrow cells receive any stimulation for the self-replication and differentiation from normal 'stromal' cell colonies in the bone marrow cell culture in vitro. When irradiated or unirradiated bone marrow cells were overlaid on the normal adherent cell colonies, the proliferation of haemopoietic stem cells was supported, the degree of the stimulation depending on the starting cellular concentration. There was, however, no significant changes in the concentration of either CFUs or CFUc regardless of the dose of irradiation on the bone marrow cells overlaid. This was a great contrast to the dose-dependent decrease of CFUs or CFUc within the culture in which both the stem cells and stromal cells were simultaneously irradiated. These results suggest that the balance of self-replication and differentiation of the haemopoietic stem cells is affected only when ...

1981-09-01

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281

British Library Electronic Table of Contents (United Kingdom)

Abstract Here we propose a software for the estimation of the occupied area and volume of fungal cultures. This software was developed using a Matlab platform and allows analysis of high-definition images from optical, electronic or atomic force microscopes. In a first step, a single hypha grown on potato dextrose agar was monitored using optical microscopy to estimate the change in occupied area and volume. Weight measurements were carried out to compare them with the estimated volume, revealing a slight difference of less than 1.5%. Similarly, samples from two different solid-state fermentation cultures were analyzed using images from a scanning electron microscope (SEM) and an environmental SEM (ESEM). Occupied area and volume were calculated for both samples, and the results obtained w...

2011-01-01

282

Developing defined culture systems for human pluripotent stem cells

British Library Electronic Table of Contents (United Kingdom)

Human pluripotent stem cells hold promising potential in many therapeutics applications including regenerative medicine and drug discovery. Over the past three decades, embryonic stem cell research has illustrated that embryonic stem cells possess two important and distinct properties: the ability to continuously self-renew and the ability to differentiate into all specialized cell types. In this article, we will discuss the continuing evolution of human pluripotent stem cell culture by examining requirements needed for the maintenance of self-renewal in vitro. We will also elaborate on the future direction of the field toward generating a robust and completely defined culture system, which has brought forth collaborations amongst biologists and engineers. As human pluripotent stem cell re...

2011-01-01

283

Coloring of cultured pearls by gamma-rays irradiation

Cell culture and gene transcription effects of copper sulfate on Chinese hamster ovary cells

Changing cream pearls into bluish-grey by #gamma# ray irradiation is a technique in coloring of pearls. Irradiated pearls are similar in color to cultured blue pearls. The pearl layers hardly change their color but the nuclei change into dark brown by irradiation. Visible light (500 - 700 nm) penetrating the pearl layer is absorbed by dark brown nucleus. The intensity of reflecting light between 400 and 500 nm at pearl surface, therefore, becomes stronger than that between 500 and 700 nm; therefore color of irradiated pearls look bluish-grey. The density of bluish-grey color increases with increasing absorbed doses, but their luster at surface diminishes owing to the deterioration of the pearl layer by prolonged irradiation; high doses irradiation should be avoided. Irradiated pearls show no substantial fading of their color in a year and the rate of the fading is found to be lower than that for cultured blue pearls. (author).

284

British Library Electronic Table of Contents (United Kingdom)

Abstract This study reports the effects of varying concentrations of copper sulfate on the metabolic and gene transcriptional profile of a recombinant Chinese hamster ovary (CHO) cell line producing an immunoglobulin G (IgG)-fusion protein (B0). Addition of 50 M copper sulfate significantly decreased lactate accumulation in the cultures while increasing viable cell density and protein titer. These changes could be seen from day 6 and became increasingly evident with culture duration. Reducing the copper sulfate concentration to 5 M retained all the above beneficial effects, but with the added benefit of reduced levels of the aggregated form of the B0 protein. To profile the cellular changes due to copper sulfate addition at the transcriptional level, Affymetrix CHO microarrays were used to...

2011-01-01

285

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

British Library Electronic Table of Contents (United Kingdom)

In the present study, human neonatal fibroblasts were isolated from a two-month-old human male. The purpose of the present investigation was the analysis of the morphology (light and transmission electron microscopy), karyotype and growth characteristics of the human neonatal fibroblast cell culture B-HNF-1. Moreover, STR typing and mitochondrial DNA amplification and sequencing was also performed. Analysis of chromosomes count showed that B-HNF-1 cell culture is diploid and has normal male karyotype 46, XY, which was stable during cultivation. The transmission electron microscopy demonstrated the ultra-structure of the B-HNF-1 cells; they have typical morphological features of proteosynthesis-active cells. Large number of fibroblasts bearing different shapes and surface characteristics ad...

2010-01-01

286

A cultural model of household energy consumption

Treatment of asymptomatic internal resorption of a maxillary premolar tooth in a military working dog.

In this paper, we consider the development of demand-side research, from an early interest in conservation behavior to a later focus on physical, economic, psychological and social models of energy consumption. Unfortunately, none of these models account satisfactorily for measured energy consumption in the residential sector. Growing interest in the end-uses of energy (e.g. in support of load forecasting, demand-side management and least-cost utility planning), increasing international studies of energy use, and continuing work in the energy and lifestyles research tradition now support an emerging cultural perspective on household energy use. The ecological foundations of the cultural model and its applications in energy research are discussed, along with some of the analytic consequences of this approach. (author).

287

The Aldous-Shields model revisited (with application to cellular ageing)

An asymptomatic pink discoloration of a maxillary right fourth premolar tooth was discovered during a routine oral examination on a 9 year-old Belgian Malinois dog. A radiolucent lesion was seen in the pulpal chamber on radiographic examination. The lesion had perforated the mesiobuccal root of the tooth. The primary differential diagnosis was idiopathic internal resorption. The tooth was treated by partial resection (removal of the mesiobuccal root and associated crown). A vital pulpotomy and amalgam restoration was performed on the remaining tooth structure. A follow-up 1 year later demonstrated a successful treatment outcome. The animal was asymptomatic and able to perform military duties. Clinical and radiographic signs of healing were evident and the tooth was functional. PMID:10518874

1998-12-01

288

Physiological and antioxidant responses of Mentha pulegium (Pennyroyal) to salt stress

In Aldous and Shields (1988), a model for a rooted, growing random binary tree was presented. For some c>0, an external vertex splits at rate c^(-i) (and becomes internal) if its distance from the root (depth) is i. For c>1, we reanalyse the tree profile, i.e. the numbers of external vertices in depth i=1,2,.... Our main result are concrete formulas for the expectation and covariance-structure of the profile. In addition, we present the application of the model to cellular ageing. Here, we assume that nodes in depth h+1 are senescent, i.e. do not split. We obtain a limit result for the proportion of non-senescent vertices for large h.

2010-01-01

289

British Library Electronic Table of Contents (United Kingdom)

Mentha pulegium L. is a medicinal and aromatic plant belonging to the Labiatae family present in the humid to the arid bioclimatic regions of Tunisia. We studied the effect of different salt concentrations on plant growth, mineral composition and antioxidant responses. Physiological and biochemical parameters were assessed in the plant organs after 2?weeks of salt treatment with 25, 50, 75 and 100?mM NaCl. Results showed that, growth was reduced even by 25?mM, and salt effect was more pronounced in shoots (leaves and stems) than in roots. This growth decrease was accompanied by a restriction in tissue hydration and K+ uptake, as well as an increase in Na+ levels in all organs. Considering the response of antioxidant enzymes to salt, leaves and roots reacted differently to saline conditions...

2010-01-01

290

Low energy ion scattering study of palladium films on silicon(111)-7 x 7 surfaces

Labelling of "1"4C-M-ISP and its absorption, translocation and distribution in peanut and soybean seedling

The initial growth process and surface structure of thin Pd(silicide) films on clean Si(111)-7x7 surfaces have been studied by low energy ion scattering (ISS) and LEED-Auger techniques. Considerable reaction between Pd and Si at room temperature is observed to extend up to 25 ML thickness of deposited Pd. Heat treatment of the room temperature film produced epitaxial silicide Pd_2Si(0001) films covered with the accumulated elementary Si layers of 1-2 ML thickness. Deposition of 1/3 ML Pd onto a heated substrate gives a Pd-embedded ordered surface of Si(111)-#sq root#3x#sq root#3R30"0, the feature being similar to the cases of Ag, Au/Si(111) systems. (orig.).

291

Inhibition of prepenetration processes of the powdery mildew Golovinomyces orontii on host inflorescence stems is reduced in the Arabidopsis cuticular mutant cer3 but not in cer1

Labelling of "1"4C-M-ISP and its absorption, translocation and distribution in peanut and soybean seedling were studied. The results of experiments showed that the radioactive substance could be translocated in xylem and phloem of two kinds of plants. The root of peanut seedling absorbed "1"4C-M-ISP from the nutrient solution more strong than the root of soybean seedling did. After leaf treatment with "1"4C-M-ISP, the soybean seedling absorbed more radioactive substance than peanut seedling did. But the translocation's speed of radioactive substance in pnloem of two kinds of plants were similar.

1990-01-01

292

British Library Electronic Table of Contents (United Kingdom)

The obligate biotrophic fungus Golovinomyces orontii causes powdery mildew (PM) disease on its host Arabidopsis thaliana. Most research on the G. orontii?Arabidopsis pathosystem uses rosette leaves as experimental materials, so little is known about the behavior of G. orontii on other Arabidopsis organs. We thus conducted microscopic analyses of the PM infection process on leaves, stems, fruits and roots of Arabidopsis. Adaxial and abaxial surfaces of mature rosette leaves supported G. orontii infection, but growth was somewhat suppressed on cauline leaves. Prepenetration processes (germination and appressorium formation) were strongly inhibited on stems, fruits and roots. To test the effect of alterations in the Arabidopsis cuticle on the inhibition of prepenetration processes on stems, w...

2011-01-01

293

Evaluation of Chemical Interactions of Maleic Acid with Sodium Hypochlorite and Chlorhexidine Gluconate

British Library Electronic Table of Contents (United Kingdom)

IntroductionThe elimination of microorganisms from the root canal system necessitates the use of combination of irrigating solutions to enhance their antimicrobial property. The combination of irrigants and their interaction sometimes could be detrimental to the outcome of the root canal therapy. The purposes of this study were (1) to evaluate the interaction between 7% maleic acid (MA) and 2% chlorhexidine gluconate solution (CHX) and to find out the availability of individual irrigant and (2) to determine the free available chlorine content when 7% MA was mixed with 2.5% sodium hypochlorite (NaOCl) solution. MethodsInteraction between MA and CHX was assessed by high-performance liquid chromatography. Available chlorine content in NaOCl was evaluated by the standard iodine/thiosulfate tit...

2011-01-01

294

Effect of alleviating products with different mode of action on physiology and yield of olive under drought

British Library Electronic Table of Contents (United Kingdom)

Two years old self-rooted Koroneiki olive trees (Olea europaea L.) were subjected to two irrigation regimes, i.e. the fully irrigated and the severely water stressed trees, while they were treated with three alleviating products of different mode of action. The products used were the osmolyte glycine betaine, the antioxidant Ambiol and the heat and irradiance reflecting kaolin clay particles. The effects of product application and water regime on leaf characteristics, shoot and root growth, photosynthesis, leaf compatible solids (carbohydrates) concentration and yield were evaluated. All products applied, exhibited significant alleviating action, based on the relative alleviation index. Irrigated trees exhibited greater growth than drought stressed ones, while the ameliorating products mai...

2010-01-01

295

Aluminum Tolerance in Moso Bamboo (Phyllostachys pubescens)

British Library Electronic Table of Contents (United Kingdom)

Moso bamboo (Phyllostachys pubescens) is widely distributed in the acid soil region of Southern China, where great potential of aluminum (Al) toxicity exists. To evaluate the Al tolerance of Moso bamboo, seed germination and root elongation were compared with two rice cultivars, and physical and physiological damages were examined under various levels of Al stress. Results showed that Moso bamboo seed germination was inhibited when Al concentration increased to 500 ?M, and the median lethal concentration was 2,000??M. Comparatively, the rice seed germination was not inhibited even at a concentration of 2,000??M Al. Aluminum accumulated mainly in the cell wall of root apices, and entered into protoplasts as treating time prolonged and/or Al concentration increased, which resulted in apoptos...

2011-01-01

296

Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L.) with a fungal phytase gene improves phosphorus acquisition

British Library Electronic Table of Contents (United Kingdom)

A phytase gene (phyA), isolated from Aspergillus ficuum (AF537344), was introduced into cotton (Gossypium hirsutum L.) by Agrobacterium-mediated transformation to increase the phosphorus (P) acquisition efficiency of cotton. Southern and Northern blot analyses showed that the phyA was successfully incorporated into the cotton genome and expressed in transgenic lines. After growing for 45?days with phytate (Po) as the only P source, the shoot and root dry weights of the transgenic plants all increased by nearly 2.0-fold relative to those of wild-type plants, but were similar to those of transgenic plants supplied with inorganic phosphorus. The phytase activities of root extracts prepared from transgenic plants were 2.4- to 3.6-fold higher than those from wild-type plants, and the extracellu...

2011-01-01

297

A spectre haunts evolution: Haeckel, Heidegger, and the all-too-human history of biology.

A novel process for extraction of natural sweetener from licorice (Glycyrrhiza glabra) roots

Since The Meaning of Evolution (1992), Robert J. Richards has argued that modern evolutionary theory is rooted in late 18th-century Romantic science. The publication of The Tragic Sense of Life (2009) provides a fitting occasion to evaluate how this perspective revises the standard history of biological thought. This essay focuses on three aspects of Richards's attempt to rehabilitate the reputation of German Naturphilosophie: (1) the identification of Romantic strains in Charles Darwin's portrait of evolutionary history; (2) the demonstration that any attempt to treat Ernst Haeckel as a "pseudo-Darwinian" inevitably renders Darwin himself a "pseudo-Darwinian"; and (3) the denial of Haeckel's alleged responsibility for the rise of Nazi racial hygiene. This article examines Richards's case for clearing Haeckel's name, as well as the subsequent (slanderous) charge from Daniel Gasman that Richards is guilty of whitewashing the Haeckelian roots of ...

2010-01-01

298

British Library Electronic Table of Contents (United Kingdom)

Pressurized hot water extraction (PHWE) is a very useful technique for recovering bioactive molecules from natural materials using subcritical compressed liquid water in the temperature range of 50-150degreeC. A novel process has been developed for making a natural sweetener from licorice (Glycyrrhiza glabra) roots involving selective chemical reaction and easy separation, for recovering mono-ammonium glycyrrhizate (MAG) using hot water with dissolved ammonia and pressurized with carbon dioxide. The performance of the novel PHWE process has been evaluated to ascertain the optimum process parameters to maximize the recovery of MAG by varying the parameters, such as temperature (30-120degreeC), pressure (1-10atm), extraction time (60-120min), water-to-feed ratio (20-40ml/g), number of stages...

2008-01-01

299

Zinc release from thapsigargin/IP3-sensitive stores in cultured cortical neurons

Why do men marry and why do they stray?

BackgroundChanges in ionic concentration have a fundamental effect on numerous physiological processes. For example, IP₃-gated thapsigargin sensitive intracellular calcium...Full Text Available

300

Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)

Humans are quite unusual compared to other great apes in that reproduction typically takes place within long-term, iteroparous pairings—social arrangements that have been culturally reified...Full Text Available

2007-07-07

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301

Use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of Campylobacter jejuni and Campylobacter coli.

BackgroundSelection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream (Sparus aurata)...Full Text Available

302

Use of Forward Genetics to Discover Novel Regulators of NF-?B

A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to...Full Text Available

1990-07-01

303

Urinary tract infection in children.

Forward and reverse genetic experiments have both played important roles in revealing critical aspects of mammalian signal transduction pathways in cell culture experiments. Only recently have we begun...Full Text Available

2010-06-01

304

Tissue-engineered product: allogeneic cultured dermal substitute composed of spongy collagen with fibroblasts.

During 1968-77, 572 consecutive children with one or more positive urine cultures who were referred by their family doctors to one paediatric surgical outpatient clinic were investigated and prospectively...Full Text Available

1984-08-04

305

Tick-Borne Relapsing Fever in British Columbia, Canada: First Isolation of Borrelia hermsii

Recently, various types of allogeneic skin substitutes including cultured epidermal substitute (CES), cultured dermal substitute (CDS), and cultured skin substitute (CSS), which are composed of keratinocytes and/or fibroblasts as the cellular component(s), have been used as biological wound dressings. In our study, the allogeneic CDS was prepared by plating fibroblasts on a spongy collagen. The clinical evaluation was conducted using fresh or cryopreserved allogeneic CDS. In 145 of our clinical cases, 95% (138/145) of various wounds were evaluated as achieving good or excellent results, including 96% (22/23) of deep dermal burns (DDB) and dermal burns (DB), 100% (53/53) of partial-thickness donor wounds, 91% (21/23) of traumatic skin defects, 100% (5/5) of pressure ulcers, 82% (9/11) of chronic skin ulcers, 100% (6/6) of coverage for debrided DB, and 92% (22/24) of coverage for autologous meshed graft. The results obtained ...

2001-03-01

306

Three-dimensional culture models of mammary gland

The spirochete that causes tick-borne relapsing fever, Borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern...Full Text Available

1998-12-01

307

The protective antigens of equine herpesvirus type 1.

The mammary gland is a complex tissue comprised of a branching network of ducts embedded within an adipocyte-rich stroma. The ductal epithelium is a bi-layer of luminal and myoepithelial cells, the...Full Text Available

2009-04-01

308

The Biosynthesis of ?-Aminolevulinic Acid in Chlorella¹

Equine herpesvirus type 1 was cultivated in swine testis cell cultures and partially purified by differential centrifugation and centrifugation in a linear sucrose density gradient. The viral envelope...Full Text Available

1978-04-01

309

Sugarcane pests and their management

When autotrophically growing cultures of Chlorella are treated with levulinic acid, δ-aminolevulinic acid is excreted into the medium, providing a direct demonstration of α-aminolevulinic...Full Text Available

1970-04-01

310

Successful establishment of primary small airway cell cultures in human lung transplantation

This book chapter discusses sugarcane culture and history, describes arthropod biologies and injury, and identifies sugarcane pest management factors to consider for people interested in commercial sugarcane production. Arthropod groups include 10 orders and 40 families. Sugarcane pest management ...

311

Stabilization of lignin peroxidases in white rot fungi by tryptophan.

BackgroundThe study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway function...Full Text Available

2009-01-01

312

Stability of Trichomonas vaginalis DNA in Urine Specimens?

Supplementation of various cultures of white rot fungi with tryptophan was found to have a large stimulatory effect on lignin peroxidase activity levels. This enhancement was greater than that observed...Full Text Available

1997-07-01

313

Spoligotype Profile of Mycobacterium tuberculosis Complex Strains from HIV-Positive and -Negative Patients in Nigeria: a Comparative Analysis ?

Trichomonas vaginalis is an important pathogen in both men and women. Culture is considered the diagnostic gold standard, although studies have shown that PCR is more sensitive than...Full Text Available

2008-05-01

314

Sources of salmonellae in an uninfected commercially-processed broiler flock.

We ran a comparative analysis of all patients for whom a positive culture of Mycobacterium tuberculosis complex was available between April 2004 and October 2005 and whose HIV serology...Full Text Available

2011-01-01

315

Some characteristics of a secreted chlamydial antigen recognized by IgG from C. trachomatis patient sera.

Cultural monitoring was used to study the incidence and sources of salmonellae in a 4160 bird broiler flock during the growing period, transport and processing in a commercial plant. No salmonellae...Full Text Available

1980-07-01

316

Self-management among Patients Living with Diabetes in the United States Virgin Islands

Chlamydia trachomatis serovars release a glycolipid antigen (GLXA) into the culture supernatant during the infective cycle. This antigen is recognized by IgG isolated from humans with a natural chlamydial...Full Text Available

1989-12-01

317

Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.

The United States Virgin Islands (USVI) is facing a diabetes epidemic similar to the one on the U.S. mainland, yet little is known regarding the cultural context relevant to self-management...Full Text Available

2011-01-01

318

Role-Reversal Exercise with Deaf Strong Hospital to Teach Communication Competency and Cultural Awareness

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein...Full Text Available

1992-07-01

319

Response to a trial of physician-based inpatient order entry.

ObjectiveTo implement a role-reversal exercise to increase first-year pharmacy students' awareness of communication barriers in the health care setting, especially for deaf and hard-of-hearing...Full Text Available

2011-04-11

320

Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy

Our group has developed a physician-operated inpatient order-entry system (BICS-OE). Mindful of the problems inherent in bringing a radical cultural change such as this to the hospital, we conducted...Full Text Available

1993-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Pyogenic Spondylodiscitis after Percutaneous Endoscopic Lumbar Discectomy

BackgroundThe circadian rhythm in mammals is orchestrated by a central pacemaker in the brain, but most peripheral tissues contain their own intrinsic circadian oscillators. The...Full Text Available

322

Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui.

We reviewed 9 cases of pyogenic spondylodiscitis following percutaneous endoscopic lumbar discectomy (PELD). Microbiologic cultures revealed 6 causative organisms. Five patients were managed conservatively...Full Text Available

2010-11-01

323

Psychometric Properties of the KPAS in Diverse Ethnic Groups of Midlife Women

An enzyme active against carboxymethyl cellulose (CMC) was purified from the stationary-phase-culture supernatant of Clostridium josui grown in a medium containing ball-milled cellulose. The purification...Full Text Available

1989-07-01

324

Proteome of human colon cancer stem cells: A comparative analysis

Although the Kaiser Physical Activity Survey (KPAS) was a potential instrument for cross cultural research of midlife women, little information is available on its reliability and validity among...Full Text Available

2009-12-01

325

Production of placental alkaline phosphatase (PLAP) and PLAP-like material by epithelial germ cell and non-germ cell tumours in vitro.

AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.METHODS: SW1116 cells were isolated and cultured with...Full Text Available

2011-03-14

326

Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran

Placental and placental-like alkaline phosphatase (PLAP) levels in the culture media of 87 cell lines of neoplastic and 'normal' origin were measured by a conventional immunosorbent enzymatic assay...Full Text Available

1994-02-01

327

British Library Electronic Table of Contents (United Kingdom)

Solid-state fermentation of soybean hulls supplemented with wheat bran using a co-culture of Trichoderma reesei and Aspergillus oryzae was performed. Three parameters - initial moisture content, incubation temperature, and initial pH - were optimized in culture flasks using response surface methodology. Parameter optimization was carried out with respect to filter paper activity and b-glucosidase activity in the culture. Temperature of 30^oC, pH of 5, and moisture content of 70% were found to be optimum. Optimized parameters were used for laboratory scale-up in static tray fermenters. The maximum filter paper activity of 10.7FPU/g-ds and b-glucosidase of 10.7IU/g-ds were obtained after 96-h incubation period in static tray fermenters in agreement with optimized activities at shake flask le...

2010-01-01

328

Product toxicity and cometabolic competitive inhibition modeling of chloroform and trichloroethylene transformation by methanotrophic resting cells.

Plasmid Transfer between Bacillus thuringiensis subsp. israelensis Strains in Laboratory Culture, River Water, and Dipteran Larvae

The rate and capacity for chloroform (CF) and trichloroethylene (TCE) transformation by a mixed methanotrophic culture of resting cells (no exogenous energy source) and formate-fed cells were measured....Full Text Available

1991-04-01

329

Phytochelatin Synthesis and Glutathione Levels in Response to Heavy Metals in Tomato Cells ¹

Plasmid transfer between strains of Bacillus thuringiensis subsp. israelensis was studied under a range of environmentally relevant laboratory conditions in vitro,...Full Text Available

2001-01-01

330

Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes

Cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, produce phytochelatins (poly[γ-glutamylcysteinyl]glycines) when exposed to cadmium. The synthesis...Full Text Available

1987-12-01

331

Oil palm (Elaeis guineensis Jacq.) tissue culture ESTs: Identifying genes associated with callogenesis and embryogenesis

The intercalated disk (ID) is a complex structure that electromechanically couples adjoining cardiac myocytes into a functional syncitium. The integrity of the disk is essential for normal cardiac function,...Full Text Available

2010-01-01

332

Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation.

BackgroundOil palm (Elaeis guineensis Jacq.) is one of the most important oil bearing crops in the world. However, genetic improvement of oil palm through conventional...Full Text Available

333

Mortality, Recruitment and Change of Desert Tree Populations in a Hyper-Arid Environment

A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its...Full Text Available

1975-03-01

334

Modeling the emergence of universality in color naming patterns

BackgroundLong-term vegetation changes in hyper-arid areas have long been neglected. Mortality, recruitment and change in populations of the ecologically and culturally important...Full Text Available

335

Microbial transformation of artificial estrogens of the allenolic group.

The empirical evidence that human color categorization exhibits some universal patterns beyond superficial discrepancies across different cultures is a major breakthrough in cognitive science. As observed...Full Text Available

2010-02-09

336

Mesophyll Cell Protoplasts of Potato

When 2,2-dimethyl 3-(2'-naphthyl 6'-hydroxy) pentanoic acid, an artificial estrogen of the allenolic acid group, was added to an exponential-phase growth culture of Neurospora crassa (in Horowitz medium),...Full Text Available

1975-06-01

337

Medical disclosure and refugees. Telling bad news to Ethiopian patients.

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among...Full Text Available

1977-08-01

338

Mapping the cellular and molecular heterogeneity of normal and malignant breast tissues and cultured cell lines

The strong value in American medical practice placed on the disclosure of terminal illness conflicts with the cultural beliefs of many recent refugees and immigrants to the United States, who often...Full Text Available

1992-09-01

339

Mapping the Spatiotemporal Dynamics of Calcium Signaling in Cellular Neural Networks Using Optical Flow

IntroductionNormal and neoplastic breast tissues are comprised of heterogeneous populations of epithelial cells exhibiting various degrees of maturation and differentiation. While...Full Text Available

2010-01-01

340

Light and Dark Controls of Nitrate Reduction in Wheat (Triticum aestivum L.) Protoplasts ¹

An optical flow gradient algorithm was applied to spontaneously forming networks of neurons and glia in culture imaged by fluorescence optical microscopy in order to map functional calcium signaling...Full Text Available

2010-08-01

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341

Isolation of gram-positive rods that resemble but are clearly distinct from Actinomyces pyogenes from mixed wound infections.

Protoplasts were isolated from the leaves of nitrate-cultured wheat (Triticum aestivum L. var. Frederick) seedlings. When incubated in the dark, protoplasts accumulated nitrite under...Full Text Available

1982-02-01

342

Isolation and Characterization of an H₂-Oxidizing Thermophilic Methanogen

Beginning in 1990, gram-positive rods resembling Actinomyces pyogenes were found with increasing frequency in mixed cultures from various infectious processes, most of them from patients with otitis,...Full Text Available

1993-05-01

343

Intra- and extracellular calcium modulates stereocilia stiffness on chick cochlear hair cells.

A thermophilic methanogen was isolated from enrichment cultures originally inoculated with sludge from an anaerobic kelp digester (55°C). This isolate exhibited a temperature optimum of 55 to...Full Text Available

1983-01-01

344

Interface of culture, insecurity and HIV and AIDS: Lessons from displaced communities in Pader District, Northern Uganda

Segments of the chick basilar papilla were isolated and maintained in culture medium. The sensory hair bundle of individual hair cells was observed with light microscopy and stimulated with a water...Full Text Available

1994-02-01

345

Inoculation of Scytalidium thermophilum in Button Mushroom Compost and Its Effect on Yield

BackgroundNorthern Uganda unlike other rural regions has registered high HIV prevalence rates comparable to those of urbanized Kampala and the central region. This could be due to...Full Text Available

346

Increase of unsaturated fatty acids under high pressure in a deep-sea bacterium; Shinkai kara bunrisareta taiatsusei biseibutsu DSS12 kabu no baiyo ondo, baiyo atsuryoku to kintai shibosan sosei no henka

Scytalidium thermophilum isolates in culture, as well as the endogenous strain(s) in mushroom compost, were inactivated at 70°C. This temperature was used to pasteurize composts...Full Text Available

1994-09-01

347

In vitro studies on the adjuvanticity of Brucella fractions.

A cellular membrane exchanges substances into and from the cell and protects the cell interior by maintaining semi-fluidity rather than be being solid. It is known that microorganisms maintain the fluidity in correspondence with varying environmental temperatures by changing and adjusting the composition of fatty acids which constitute the cellular membrane lipids. As part of the studies to elucidate the pressure withstanding mechanism in deep-sea bacteria, this paper investigates what variations the fatty acid composition, which constitutes cellular membranes of the DSS12 strain, a pressure withstanding bacterium growing well under either normal pressure or high pressures, will show under different culturing temperatures and pressures. Culture under low temperatures and culture under high pressures increase content of unsaturated fatty acids including icosapentanoic acid. Culture under high ...

1997-03-01

348

Immunoquantitative Real-Time PCR for Detection and Quantification of Staphylococcus aureus Enterotoxin B in Foods

Two Brucella fractions, the murein-linked fraction PI and the murein-free fraction SF, behave as in vitro adjuvants for primary anti-sheep erythrocyte responses: added to Mishell and Dutton-type cultures...Full Text Available

1982-11-01

349

Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture

A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay...Full Text Available

2006-10-01

350

Hexadecane mineralization in oxygen-controlled sediment-seawater cultivations with autochthonous microorganisms.

During a study on the mTor pathway in the rat kidney we observed a striking increase of the phosphorylation of the S6 kinase in mitosis. In cryostat sections of perfusion-fixed tissue mitotic cells...Full Text Available

2007-02-01

351

H-1152 Effects on Intraocular Pressure and Trabecular Meshwork Morphology of Rat Eyes

Laboratory studies investigated the influence of dissolved oxygen tension (DOT) on microbial degradation of hexadecane in cultures with sediment-seawater suspensions. With a fermentor system, it was...Full Text Available

1992-09-01

352

Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy

AbstractPurposeThe aim of this study was to elucidate the effects of the Rho-kinase inhibitor, H-1152, on cultured human trabecular meshwork (HTM) cells, TM morphology,...Full Text Available

2008-08-01

353

Germline mutagenesis mediated by Sleeping Beauty transposon system in mice

This work reports the design of and experimentation with a topographically patterned cell culture substrate of variable local density and anisotropy as a facile and efficient platform to guide...Full Text Available

2009-02-06

354

Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc

Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice....Full Text Available

2007-01-01

355

Gendered Technoculture: Sex, Lies, Videotape & Cyberspace

Zinc is an essential trace metal ion for growth, but an excess of Zn is toxic and microorganisms express diverse resistance mechanisms. To understand global bacterial responses to excess Zn, we conducted...Full Text Available

2005-02-01

356

Flow Cytometry of Human Primary Epidermal and Follicular Keratinocytes

What happens in the matrix of gender, technology, representation, and culture? This class will explore the theoretical issues raised within this matrix. We will also make sense of the theories through practical experiences with technologies like computers and cameras.

2004-02-01

357

Flock infection and transport as sources of salmonellae in broiler chickens and carcasses.

Objective: The aim of this study was to characterize using flow cytometry cultured human primary keratinocytes isolated from the epidermis and hair follicles by different methods. Methods:...Full Text Available

358

First Culture Isolation of Borrelia lonestari, Putative Agent of Southern Tick-Associated Rash Illness

Cultural monitoring was used to determine the incidence and sources of salmonellae in a 4160-bird broiler flock raised on litter in 32 pens. Twenty-five of the pens remained apparently free of salmonellae...Full Text Available

1980-07-01

359

Evidence that cell surface heparan sulfate is involved in the high affinity thrombin binding to cultured porcine aortic endothelial cells.

Southern tick-associated rash illness (STARI) is a Lyme disease-like infection described in patients in the southeastern and south-central United States, where classic Lyme disease is relatively rare....Full Text Available

2004-03-01

360

Evidence That Two Major Replicons Comprise the Genome of Staphylococcus Aureus.

It has been postulated that thrombin binds to endothelial cells through, at least in part, cell surface glycosaminoglycans such as heparan sulfate, which could serve as antithrombin cofactor on the...Full Text Available

1985-04-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Evaluation of the Widal tube agglutination test for the diagnosis of typhoid fever among children admitted to a rural hdospital in Tanzania and a comparison with previous studies

In Staphylococcus aureus, a pronounced shift in position of the acriflavin resistance locus was observed when gene order was determined by marker frequency analysis of cells of various ages. In young cells (2-hour culture), acriflavin resistance was mappe...

1967-01-01

362

Epidermal keratinocytes do not activate peripheral T-cells: interleukin-10 as a possible regulator

BackgroundThe diagnosis of typhoid fever is confirmed by culture of Salmonella enterica serotype Typhi (S. typhi). However, a more rapid, simpler,...Full Text Available

363

Enzyme amplified immunoassay: a novel technique applied to direct detection of Chlamydia trachomatis in clinical specimens.

The immunogenicity of allogeneic cultured human epidermal keratinocytes (cHEKs) has been studied in several models with contradictory results. We studied human T-cell activation in an in vitro...Full Text Available

2008-11-01

364

Endometrial biopsy in Holstein-Friesian dairy cows. III. Bacteriological analysis and correlations with histological findings.

Endocervical swabs from 212 women and urethral swabs from 100 men were tested by the routine methods for McCoy cell culture and simultaneously by a novel enzyme amplified immunoassay test to detect...Full Text Available

1985-10-01

365

Effect of pH and Temperature on Denitrification Gene Expression and Activity in Pseudomonas mandelii?

This study examines the results of bacterial culture from 159 endometrial biopsy samples from 97 commercial dairy cows and correlations between bacteriological and histological findings. Bacteria were...Full Text Available

1991-04-01

366

Development of rat CA1 neurones in acute Versus organotypic slices: role of experience in synaptic morphology and activity

Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR....Full Text Available

2009-06-01

367

Detection of cellular responses to toxicants by dielectrophoresis

Despite their wide use, the physiological relevance of organotypic slices remains controversial. Such cultures are prepared at 5 days postnatal. Although some local circuitry remains intact, they develop...Full Text Available

2003-07-01

368

Detection of Unculturable Bacteria in Periodontal Health and Disease by PCR

The dielectrophoretic (DEP) crossover method has been applied to the detection of cell responses to toxicants. Time and dose responses of the human cultured leukemia (HL-60) line were measured...Full Text Available

2002-08-31

369

Detection of Chlamydia trachomatis in genital swabs: comparison of commercial and in house amplification methods with culture

Recently developed molecular methods have made it possible to characterize mixed microflora in their entirety, including the substantial numbers of bacteria which do not grow on artificial culture media....Full Text Available

1999-05-01

370

Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.

AIMS: To evaluate the sensitivity of the Roche Cobas, Roche Amplicor plate kit, ligase chain reaction (LCR), and an in house polymerase chain reaction (PCR) by titration of purified elementary bodies...Full Text Available

1998-08-01

371

Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures...Full Text Available

1991-07-01

372

British Library Electronic Table of Contents (United Kingdom)

In the present study mixed cultures that could grew in the molasses media were isolated from textile dye effluent and its decolorization activity was studied in a batch system under anaerobic conditions, in order to determine the optimal conditions required for the highest decolorization activity. The optimum pH value for decolorization was determined as 8 for all the dyes tested. In the experiment with pH 8 dye decolorizations by mixed cultures were investigated at about 96.2-1031.3mgl-1 initial dye concentrations. The highest dye removal rates of mixed cultures were 94.9% for Reactive Red RB, 91.0% for Reactive Black B and 63.6% for Remazol Blue at 953.2, 864.9 and 1031.3mgl-1 initial dye concentrations respectively within 24h incubation period. When the Reactive Red RB was used, approxi...

2006-01-01

373

Cytoplasmic heat shock granules are formed from precursor particles and are associated with a specific set of mRNAs.

Cytokinins and Flower Bud Formation in Vitro in Tobacco

In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger...Full Text Available

1989-03-01

374

Cytochrome c-d regulates developmental apoptosis in the Drosophila retina

Explants from flower stalks of Nicotiana tabacum L. were cultured on different cytokinins to induce flower bud formation. All cytokinins tested except zeatin and zeatin-riboside induced...Full Text Available

1990-03-01

375

Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator

The role of cytochrome c (Cyt c) in caspase activation has largely been established from mammalian cell-culture studies, but much remains to be learned about its physiological...Full Text Available

2006-09-01

376

Corticosteroid can alter antigen expression on alveolar macrophages.

Curcumin is a natural product currently in human clinical trials for a variety of neoplastic, preneoplastic, and inflammatory conditions. We previously observed that, in cultured cells, curcumin exhibits...Full Text Available

2009-01-08

377

Comparative Transcriptional and Genomic Analysis of Plasmodium falciparum Field Isolates

Normal healthy volunteers underwent broncho-alveolar lavage and the cells obtained were cultured for 24 h and 48 h, either alone or in the presence of the corticosteroid, Budesonide. Cell differentials...Full Text Available

1991-09-01

378

Cloning and expression of the gene for the Avi-3 antigen of Mycobacterium avium and mapping of its epitopes.

Mechanisms for differential regulation of gene expression may underlie much of the phenotypic variation and adaptability of malaria parasites. Here we describe transcriptional variation among culture-adapted...Full Text Available

2009-10-01

379

Charge compensation for NADPH oxidase activity in microglia in rat brain slices does not involve a proton current

The Avi-3 antigen, which is found only in Mycobacterium avium culture sonic extracts, is species specific and results in strong skin test activity in guinea pigs sensitized with heat-killed M. avium....Full Text Available

1992-03-01

380

Characterization of Cytokinin and Adenine Transport in Arabidopsis Cell Cultures1[OA

The membrane properties of isolated cultured microglia have been extensively studied but it is important to understand their properties in situ, where they protect the brain against...Full Text Available

2008-09-01

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381

Cell resilience in species lifespans: a link to inflammation?

Cytokinins are distributed through the vascular system and trigger responses of target cells via receptor-mediated signal transduction. Perception and transduction of the signal can occur at the plasma...Full Text Available

2008-12-01

382

Biosynthetic Pathway of Insect Juvenile Hormone III in Cell Suspension Cultures of the Sedge Cyperus iria1

Species differences in lifespan have been attributed to cellular survival during various stressors, designated here as ‘cell resilience’. In primary fibroblast cultures, cell...Full Text Available

2010-08-01

383

Biological, Life Course, and Cross-Cultural Studies All point Toward the Value of Dimensional and Developmental Ratings in the Classification of Psychosis

In most insect species, juvenile hormones regulate critical physiological processes such as metamorphosis and reproduction. In insects, these sesquiterpenoids are synthesized by retrocerebral endocrine...Full Text Available

2001-10-01

384

Biological Response Modifier Activity of an Exopolysaccharide from Paenibacillus jamilae CP-7

The diagnostic criteria for schizophrenia in the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV1)...Full Text Available

2007-07-01

385

Attachment Stimulates Exopolysaccharide Synthesis by a Bacterium

An extracellular polysaccharide was purified from culture supernatants of Paenibacillus jamilae CP-7, a gram-positive bacillus that was isolated from compost prepared with olive mill...Full Text Available

2001-07-01

386

Apoptosis of human seminoma cells upon disruption of their microenvironment.

This study examined the hypothesis that solid surfaces may stimulate attached bacteria to produce exopolymers. Addition of sand to shake-flask cultures seemed to induce exopolymer synthesis by a number...Full Text Available

1993-10-01

387

Albumin interacts specifically with a 60-kDa microvascular endothelial glycoprotein.

One of the main obstacles encountered when trying to culture human seminoma (SE) cells in vitro is massive degeneration of the tumour cells. We investigated whether dissociation of tumour tissue, to...Full Text Available

1996-05-01

388

A proteomic study of cMyc improvement of CHO culture

Confluent monolayers of microvascular endothelial cells, derived from the rat epididymal fat pad and grown in culture, were radioiodinated by using the lactoper-oxidase method. Their radioiodinated...Full Text Available

1988-09-01

389

A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells

BackgroundThe biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number resulting in a maximum volumetric recombinant...Full Text Available

390

A convenient and sensitive allergy test: IgE crosslinking-induced luciferase expression in cultured mast cells

Cultured mouse or human embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Chemical or insertional...Full Text Available

2009-04-01

391

A Nitrate Reductase-less Variant Isolated from Suspension Cultures of Datura innoxia (Mill.) ¹

BackgroundFor the detection of allergen-specific IgE in sera, solid-phase IgE-binding assays like the CAP test are commonly used. Although such immunochemical methods are very sensitive,...Full Text Available

2010-10-01

392

3D Hepatic Cultures Simultaneously Maintain Primary Hepatocyte and Liver Sinusoidal Endothelial Cell Phenotypes

A comparative study has been carried out of the growth of two lines of Datura innoxia (Mill.) cells, designated DI-6 and NR1, their resistance to chlorate, and their ability to assimilate...Full Text Available

1980-10-01

393

The Integration of Energy Efficiency, Renewable Energy, DemandResponse and Climate Change: Challenges and Opportunities for Evaluatorsand Planners

Developing in vitro engineered hepatic tissues that exhibit stable phenotype is a major challenge in the field of hepatic tissue engineering. However, the rapid dedifferentiation of...Full Text Available

394

Research, Preservation, and Education: An Introduction to Various Heritage Centers, Organizations, and Projects

This paper explores the feasibility of integrating energyefficiency program evaluation with the emerging need for the evaluationof programs from different "energy cultures" (demand response, renewableenergy, and climate change). The paper reviews key features andinformation needs of the energy cultures and critically reviews theopportunities and challenges associated with integrating these withenergy efficiency program evaluation. There is a need to integrate thedifferent policy arenas where energy efficiency, demand response, andclimate change programs are developed, and there are positive signs thatthis integration is starting to occur.

2007-05-29

395

British Library Electronic Table of Contents (United Kingdom)

This forum showcases the work of a variety of different heritage-based centers, organizations, and projects dedicated to research, education, and preservation of tangible and intangible forms of cultural heritage. The descriptions of these centers demonstrate the diversity of heritage work being done today. The centers and projects described in the forum vary in their contexts, missions, and outcomes. Highlighted in the forum are preservation organizations, university-based heritage centers, and a global collaborative cultural heritage project. Each organization in the forum provides information about their missions and goals, their approaches or methods to heritage work, and a brief description of some of their initiatives.

2011-01-01

396

Mechanism of biodegradation of paraquat by Lipomyces starkeyi

Mechanism of biodegradation of paraquat by Lipomyces starkeyi

The biodegradation of ring-/sup 14/C- and methyl-/sup 14/C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO/sub 2/. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

1985-05-01

397

Man-made disasters: A cross-national analysis

The biodegradation of ring-"1"4C- and methyl-"1"4C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO_2. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

398

British Library Electronic Table of Contents (United Kingdom)

This research investigates the impact of national culture and several institutional factors on the safety performance of society and establishes statistically significant relationships between those variables. As expected, the research results reveal that some cultural variables such as uncertainty avoidance, gender orientation and institutional variables such as the degree of law avoidance can directly influence the safety performance of the society. The findings also support the inverted u-curve (Safety Kuznet curve) hypothesis indicating even if we expect a negative trend at the beginning stage of industrialization, we can expect a positive trend in safety performance as their income level continues to improve beyond a certain point.

2011-01-01

399

In vitro study of plutonium in macrophages

Determination of the cell and mucous distribution in the airways of the lung

An in vitro system for studying Pu uptake by, and removal from, macrophages was developed to provide data for the actinide therapy program. We have shown that "2"3"9PuO_2 particle uptake in vitro resembles the process in vivo with respect to the intracellular localization of phagocytized particles. At the end of 7 days of culture of Pu-loaded cells, 54% of the cells were viable even though 71% of the cells in the culture initially contained enough "2"3"9PuO_2 to deliver more than 53 intracellular #alpha#-disintegrations.

1977-05-01

400

Tests for the diagnosis of Clostridium difficile infection: the next generation.

Models of the human airways have played a major role in evaluating the health effects of inhaled radionuclides. While models such as those of Weibel (1963) provide data necessary for characterizing deposition of aerosol, they have not characterized the cells at risks in the airspaces. Given the advancements in techniques and study of cell cultures exposed to ionizing radiation there is a need to extrapolate between the simple structures of cell culture systems and the complex architecture of the human airways. The preliminary data in this paper provide a complete characterization of the size and number of cells in the airways and represents a significant advance in our study of the health consequences of exposure to inhaled radionuclides. 26 refs., 2 figs., 4 tabs.

1991-01-01

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401

Safety evaluation of Propionibacterium freudenreichii ET-3 culture.

Clostridium difficile (C. difficile) causes 25-30% of cases of antibiotic associated diarrhea and most cases of pseudomembranous colitis. Patients presenting with diarrhea after hospitalization for 3 or more days should be tested for C. difficile. There are many options available for testing, each of which has inherent advantages and disadvantages. Most laboratories perform toxin testing using an enzyme immunoassay method. In general these tests have sensitivities ranging from 60 to 70% and specificities of 98%. When using these methods, symptomatic patients with negative tests should be tested by another more sensitive method. Until recently, cell culture cytotoxicity neutralization assays (CCNAs) were considered the gold standard in the U.S. A two-step algorithm using an EIA for glutamate dehydrogenase detection followed by testing positives using CCNA, offered an improved alternative until the availability of molecular assays. Although early studies that ...

2011-03-03

402

Regulation of sulfated glycosaminoglycan production by prostaglandin E2 in cultured lung fibroblasts

Propionibacterium freudenreichii ET-3 culture, a cell-free product of whey fermentation using P. freudenreichii ET-3 (7025), has been shown to promote the growth of Bifidobacteria through the action of 1,4-dihydroxy-2-naphthoic acid (DHNA), and therefore, has potential use in the food and supplement industries. Although currently used as a food ingredient in Japan, the safety of this novel ingredient has not been previously evaluated through traditional toxicity testing. Therefore, here we report the results of standard toxicological testing performed on P. freudenreichii ET-3 culture. In a 4-week oral toxicity study, administration of 6000mg/kg body weight/day P. freudenreichii ET-3 culture was without compound-related adverse effects on clinical signs, body weights, food consumption, ophthalmology, hematology, clinical chemistry, urinalysis, organ weights, and gross and microscopic findings in male and female ...

2011-03-22

403

Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations

Prostaglandin E2 (PGE2) has been shown to increase the synthesis of hyaluronic acid in cultured fibroblasts by increasing the activity of hyaluronate synthetase, a group of plasma membrane-bound synthetic enzymes. We examined whether PGE2 also increased the activity of those enzyme systems involved in the synthesis of sulfated glycosaminoglycan in the human embryonic lung fibroblast. Exposure of cells to PGE2 resulted in dose-dependent increases in glucosamine incorporation into all sulfated glycosaminoglycan subtypes. PGE2 at 10(-7) mol/L increased total glycosaminoglycan per dish to 21.6 +/- 3.1 micrograms versus 12.0 +/- 2.5 micrograms in control untreated cultures. Stimulation of endogenous PGE2 production by bradykinin had a similar effect on glycosaminoglycan synthesis. To examine whether PGE2 affected sulfated glycosaminoglycan protein core production, cells were labeled with tritiated glucosamine in the presence of cycloheximide. Under ...

1989-08-01

404

Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured human hepatoma cells, but save for the genotype 2a isolate JFH-1, efficient replication of these HCV RNAs requires replication enhancing mutations (REMs), previously also called cell culture adaptive mutations. These mutations cluster primarily in the central region of non-structural protein 5A (NS5A), but may also reside in the NS3 helicase domain or at a distinct position in NS4B. Most efficient replication has been achieved by combining REMs residing in NS3 with distinct REMs located in NS4B or NS5A. However, in spite of efficient replication of HCV genomes containing such mutations, they do not support production of infectious virus particles. By using the genotype 1b isolate Con1, in this study we show that REMs interfere with HCV ...

2009-06-12

405

The origin of the epithelial and myoepithelial cells in the human breast has not been delineated. In this study we have addressed whether luminal epithelial cells and myoepithelial cells are vertically connected, i.e., whether one is the precursor for the other. We used a primary culture assay allowing preservation of basic phenotypic traits of luminal epithelial and myoepithelial cells in culture. The two cell types were then separated immunomagnetically using antibodies directed against lineage-specific cell surface antigens into at best 100% purity. The cellular identity was ascertained by cytochemistry, immunoblotting, and 2-D gel electrophoresis. Luminal epithelial cells were identified by strong expression of cytokeratins 18 and 19 while myoepithelial cells were recognized by expression of vimentin and {alpha}-smooth muscle actin. We used a previously devised culture medium (CDM4) that allows vigorous expansion of ...

1999-02-01

406

Wool-waste as organic nutrient source for container-grown plants

Glycosaminoglycan synthesis was studied in cell populations of ultraviolet light-induced murine cutaneous fibrosarcoma cells under conditions of varying growth rates in vitro. After labeling with the precursors, /sup 3/H-glucosamine and /sup 35/SO/sub 4/, sulfated glycosaminoglycans recoverable by direct proteolysis of the culture monolayers increased approximately 5-fold on a per cell basis from sparsely populated, exponential cell cultures (greater than 85% of cells in S, G2, or M phases) to stationary cultures inhibited by high cell density (greater than 50% of cells in G1). Within this cell surface-associated material, the relative ratio of heparan sulfate to the chondroitin sulfates was approximately 60/40% under conditions of exponential growth; in the growth-arrested cultures, the reverse ratio was found. The substratum attached material, obtained from the flask surface after ethyl glycol ...

1985-08-01

407

Turgor Regulation in Osmotically Stressed Arabidopsis Epidermal Root Cells. Direct Support for the Role of Inorganic Ion Uptake as Revealed by Concurrent Flux and Cell Turgor Measurements1

A container experiment was conducted to test the hypothesis that uncomposted wool wastes could be used as nutrient source and growth medium constituent for container-grown plants. The treatments were: (1) rate of wool-waste application (0 or unamended control, 20, 40, 80, and 120 g of wool per 8-in. pot), (2) growth medium constituents [(2.1) wool plus perlite, (2.2) wool plus peat, and (2.3) wool plus peat plus perlite], and (3) plant species (basil and Swiss chard). A single addition of 20, 40, 80, or 120 g of wool-waste to Swiss chard (Beta vulgaris L.) and basil (Ocimum basilicum L.) in pots with growth medium provided four harvests of Swiss chard and five harvests of basil. Total basil yield from the five harvests was 1.6-5 times greater than the total yield from the unamended control, while total Swiss chard yield from the four harvests was 2-5 times greater relative to the respective unamended control. The addition of wool-waste to the growth medium increased Swiss chard and ...

2009-07-01

408

Transition rates of electrons in superheavy elements

Hyperosmotic stress is known to significantly enhance net uptake of inorganic ions into plant cells. Direct evidence for cell turgor recovery via such a mechanism, however, is still lacking. In the...Full Text Available

2002-05-01

409

Transfer of uranium and thorium from soil to different parts of medicinal plants using SSNTD

Transition rates for electrons in the superheavy elements Z = 114, 126, 134, 145, 164 and 173 are calculated. K, L and M-shells are considerd as final states. The 2s - 1s stransition of multipolarity M1 is dominant for Z = 173 with a transition time of 10"-"1"8s. The radial expectation values and #sq root# are given. (orig.).

410

The Sigma Factor AlgU (AlgT) Controls Exopolysaccharide Production and Tolerance towards Desiccation and Osmotic Stress in the Biocontrol Agent Pseudomonas fluorescens CHA0

The uptake of "2"3"8U and "2"3"2Th in different parts of some selected plants used in traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia area) has been studied using two different types of solid state nuclear track detectors (SSNTDs) LR-115 type II and CR-39. Plant uptake of radionuclides is one of many vectors for introduction of contaminants into the human food chain. Thus, it is critical to understand soil-plant relationships that control nuclide bioavailability. Soil concentrations of uranium ranged from 6.10 to 11.62 ppm, with a mean of 7.90 ppm. Soil concentrations of thorium ranged from 2.70 to 4.80 ppm, with a mean of 3.41 ppm. Mean uranium specific activities were 8.38 Bq kg"-"1 in root tissue, 5 Bq kg"-"1 in stem tissue and 6.02 Bq kg"-"1 in leaf tissue. Mean thorium specific activities were 2.53 Bq kg"-"1 in root tissue, 1.64 Bq kg"-"1 in stem tissue and 1.96 Bq kg"-"1 in leaf tissue. The transfer ...

2011-02-01

411

Simultaneous Gas Chromatography-Mass Spectrometry Quantification of Endogenous [¹²C]- and Applied [¹³C]Indole-3yl-Acetic Acid Levels in Growing Maize Roots

A variety of stress situations may affect the activity and survival of plant-beneficial pseudomonads added to soil to control root diseases. This study focused on the roles of the sigma factor AlgU...Full Text Available

2001-12-01

412

Sharp Lower Bounds on Density of Area-Minimizing Cones

The use of stable indole-3yl-acetic acid (IAA) labeled by 6 atoms of ¹³C allowed, after [¹³C]IAA treatment, simultaneous gas chromatography-mass spectrometry quantifications of...Full Text Available

1991-01-01

413

Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice

We prove that the density of a topologically nontrivial, area-minimizing hypercone with an isolated singularity must be greater than the square root of 2. The Simons' cones show that this is the best possible constant. If one of the components of the complement of the cone has nontrivial kth homotopy group, we prove a better bound in terms of k; that bound is also best possible. The proofs use mean curvature flow.

2010-01-01

414

Roles of mitochondria and temperature in the control of intracellular calcium in adult rat sensory neurons

Physiological properties involved in divergent cadmium (Cd) accumulation among rice genotypes were characterized using the indica cultivar ‘Habataki’ (high Cd in grains)...Full Text Available

2009-07-01

415

Role of Membrane-bound, Fixed-charge Changes in Phytochrome-mediated Mung Bean Root Tip Adherence Phenomenon ¹²

SUMMARYWe recorded Ca²⁺ current and intracellular Ca²⁺ ([Ca²⁺]_i) in isolated adult rat dorsal root ganglion (DRG) neurons at 20 and...Full Text Available

2008-04-01

416

Regulation of K⁺ Influx in Barley 1

The movement of cells and cell fragments in an electric field provided a means for determining the nature of cellular surface charges. We found that changes in ionic strength and particularly changes...Full Text Available

1975-03-01

417

Rapid Respiratory Changes Due to Red Light or Acetylcholine during the Early Events of Phytochrome-mediated Photomorphogenesis ¹

Influx and accumulation of K⁺ in barley (Hordeum vulgare L. cv Fergus) roots were measured at two temperatures (10°C and 20°C) in plants which had been grown...Full Text Available

1984-03-01

418

Radiation treatment of crude drugs. Development of suitable methods of detection. Strahlenbehandlung von Arzneidrogen. Entwicklung geeigneter Nachweismethoden

Two millimeter long secondary root tips of etiolated mung bean (Phaseolus aureus) plants were given 4 minute consecutive treatments of darkness, red light, far red light, and acetylcholine...Full Text Available

1972-01-01

419

Penetration effect in the M1 component of "1"7"7Ta #gamma#-transition with energy of 70.45 keV

It may be necessary to reduce microbiological contamination of crude drugs (medicinal plants or their parts like roots, leaves, flowers). This can be done by treating the drugs with ionizing radiation. Meethods for detection of such an irradiation were developed. It could be pointed out that measurements of luminescence, viscosity and electron spin resonance were suitable for specific drugs, but not for all drugs. (orig.).

1992-09-01

420

Optimal Knot Selection for Least-squares Fitting of Noisy Data with Spline Functions

The magnetic #beta#-spectrometer of the #pi# #sq root#2 type with 0.07% pulse resolution is used to measure the intensities of interval conversion electrons on L- and M-subshells of 70.45 keV "1"7"7 Ta gamma-transition. The results are analyzed and the values of mixing parameter #sigma#(E2/M1) and penetration parameter #lambda# are obtained.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

On the homology of exotic Springer fibers

An automatic data-smoothing algorithm for data from digital oscilloscopes is described. The algorithm adjusts the bandwidth of the filtering as a function of time to provide minimum mean squared error at each time. It produces an estimate of the root-mean-square error as a function of time and does so without any statistical assumptions about the unknown signal. The algorithm is based on least-squares fitting to the data of cubic spline functions.

2008-05-15

422

Metabolism and Binding of ¹⁴C-Maleic Hydrazide ¹

We determine the structure of the total homology groups of exotic Springer fibers as affine Weyl group representations. As applications, we provide single top/socle property of standard modules in the exotic Deligne-Langlands correspondence (except for root of unity case), an analogue of Verma's theorem, the coincidence of analytic/geometric gradings in the $C ^{\\infty}$-realization of anti-spherical modules of graded Hecke algebras of type $\\mathsf{BC}$ with unequal parameters, among others.

2011-01-01

423

Information technology security at the Advanced Photon Source

Maleic hydrazide (MH) is taken up by corn and pea seedling roots and bound to some material which is insoluble in 80% ethanol or 5% trichloroacetic acid. ¹⁴C-MH is stable metabolically; chromatography...Full Text Available

1970-01-01

424

Human error and experienced feedback during refuelling overhauling of Daya Bay Nuclear Power Station

The proliferation of 'botnets,' phishing schemes, denial-of-service attacks, root kits, and other cyber attack schemes designed to capture a system or network creates a climate of concern for system administrators, especially for those managing accelerator and large experimental-physics facilities, as they are very public targets. This paper will describe the steps being taken at the Advanced Photon Source (APS) to protect the infrastructure of the overall network with emphasis on security for the APS control system.

2007-10-15

425

Hair root versus red cell individual phenotype in Sardinian heterozygotes for G6PD deficiency (Mediterranean type).

The authors describe the various human error encountered during Daya bay refueling overhaul, and the subsequent root cause evaluation and protective measure. Authors consider transparency is the key in reducing human error and event recurrence. Additional y, event transparency will enhance the effectiveness if experience feedback and reduce event consequence

2000-10-01

426

Generalized supersymmetry on Riemann surfaces and the associated string models

G6PD activity was assayed in 20 Sardinian heterozygotes for G6PD deficiency and related to that of LDH and MDH. One of these heterozygotes showed a deficient phenotype in all her follicles, while the...Full Text Available

1976-09-01

427

The authors propose a generalization of the concept of supersymmetry non Riemann surfaces. Generators of this symmetry intermix M fields of different spin. Two types of statistics, i.e., bosonic and fermionic statistics, are allowed for parameters of infinitesimal transformations. They also study the possibility of string models associated with these symmetries. The algebraic structure of a part of generalized supersymmetry is regarded as a sort of an M-th root of the Virasoro algebra.

1988-11-01

428

Bibliography of prosopis

Action plan for the Tiger Team assessment report

References covering the years 1904-80 are listed under the following headings: cultivation and occurrence (India and Pakistan, Africa, South America, Pacific, Middle East); Taxonomy, morphology, variation and selection; Reference works and reviews; Ecology of Prosopis (General effects on surrounding soil and vegetation): Physiology (General, Roots, Growth, Hydrology, Saline tolerance); Control of mesquite: Propagation (Germination and other nursery techniques, Vegetative propagation): and Utilization (General, Chemical analyses, Food and Ethnobiology, Fodder, Wood, Charcoal, Gum, Paper). 141 references.

1981-01-01

429

A brief history of the World Wide Web Where it as invented, how it's used, and where it's headed

This document contains responses and planned actions that address the findings of the Tiger Team Assessment of Brookhaven National Laboratory, June 1990. In addition, the document contains descriptions of the management and organizational structure to be used in conducting planned actions, root causes for the problems identified in the findings, responses, planned actions, schedules and milestones for completing planned actions, and, where known, costs associated with planned actions.

1990-08-30

430

CERN Multimedia

The World Wide Web has its historical roots in things such as the creation of the telegraph, the launching of the Sputnik and more, but it really all started in March 1989, when Tim Berners-Lee, a computer scientist at CERN in Geneva wrote a paper called Information Management: A proposal

2005-01-01

431

Regulation of the phosphoinositide pathway in cultured Sertoli cells from immature rats: effects of follicle-stimulating hormone and fluoride

Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes

Many hormones elicit effects on target cells by stimulating the enzyme phospholipase-C, which catalyzes the hydrolysis of phosphoinositides to the intracellular second messengers diacylglycerol and inositol phosphates. The present study examined the roles of FSH and guanine nucleotide-binding proteins (G-proteins) in regulating the hydrolysis of phosphoinositides in Sertoli cells. Sertoli cell cultures prepared from 16- to 18-day-old rats were incubated for 24 h with myo-[2-3H] inositol to label endogenous phospholipids. Treatment of cells from 0.5-20 min with preparations of ovine FSH ranging in potency from 1-60 times that of NIH FSH S1 did not affect accumulation of inositol phosphates. Levels of total [3H]inositol phosphates [[3H]inositol mono-, di-, and triphosphates (IP, IP2, and IP3)] in FSH-treated cultures was 75-120% the levels in control cultures over the various time intervals studied. Addition of testosterone ...

432

Production of Shiga-like toxins by Escherichia coli O157:H7 can be influenced by the neuroendocrine hormone norepinephrine.

To study the regulation of adipose tissue lipoprotein lipase (LPL) in human adipocytes, omental adipose tissue was obtained from healthy subjects and digested in collagenase. The isolated adipocytes thus obtained were suspended in Medium 199 and cultured at 37 degrees C. Cell viability was demonstrated in adipocytes cultured for up to 72 h by constancy of cell number, cell size, trypan-blue exclusion, and specific /sup 125/I-insulin binding. In addition, chloroquine induced an increase in cell-associated /sup 125/I-insulin at 24, 48, and 72 h after preparation. Thus, isolated adipocytes retained their ability to bind, internalize, and degrade insulin. LPL was measured as activity secreted into the culture medium (CM), released from cells by heparin (HR), and extracted from cell digests. A broad range of heparin concentrations produced a prompt release of LPL from a rapidly replenishable pool of cellular activity. When cells ...

1985-01-01

433

Effect of temperature on the development of Steinernema carpocapsae and Steinernema feltiae (Nematoda: Rhabditida) in liquid culture.

To examine whether the neuroendocrine hormone norepinephrine may influence the production of the Shiga-like toxins (SLTs), several Escherichia coli O157:H7 clinical isolates were grown in the presence or absence of norepinephrine. An in vitro culture system consisting of low (<1500 colony-forming units/ml) initial concentrations of inocula into a serum-based medium was used to more closely approximate in vivo conditions. The growth of all isolates was increased several logs in the presence of norepinephrine, as compared with the growth in controls, during a 24-hour growth period. Controls included additional dextrose as well as the use of the norepinephrine metabolite normetanephrine, which contains one more methyl group than norepinephrine and hence would serve as a better energy source for growth if the effect were solely nutritionally mediated. During the 24 hours of growth, the production of cell-associated SLT-I on a protein-equivalent basis was shown to be ...

1996-10-01

434

Cultured epidermal allografts as biological wound dressings.

For commercial use of the entomopathogenic nematodes Steinernema carpocapsae and Steinernema feltiae in biological control of insect pests, they are produced in liquid culture on artificial media pre-incubated with their symbiotic bacteria Xenorhabdus nematophila and Xenorhabdus bovienii, respectively. After 1 day of the bacterial culture, nematode dauer juveniles (DJs) are inoculated, which recover development. The adult nematodes produce DJ offspring, which are harvested and can be sprayed. This study determined optimal temperatures to obtain high DJ progeny within a short process time. Temperatures assessed were 23 degrees C, 25 degrees C, 27 degrees C, and 29 degrees C for S. carpocapsae and 20 degrees C, 23 degrees C, 25 degrees C, and 27 degrees C for S. feltiae. The recovery of inoculated DJs was hardly affected and was reduced only in S. carpocapsae at 29 degrees C. The fecundity (eggs in uterus) in S. carpocapsae reached a maximum at ...

2009-05-20

435

[Aeromonas hydrophila-related septicemia in the Nile tilapia Oreochromis niloticus].

Recent advances in cell culture technology permit the generation of large stratified epithelial sheets appropriate for wound coverage. Autografts (sheets prepared from the patient's own skin) have proven life-saving in the treatment of large third-degree burns and have been successfully employed in the management of chronic ulcers. Allografts (sheets prepared from the skin of an unrelated donor) have also been used. In our experience, cultured allografts derived from neonatal foreskin provide a potent stimulus to healing in a variety of partial thickness wounds. Their application is a simple outpatient procedure which involves no discomfort for the patient. In contrast to autografting, no biopsy is necessary and use of cultured allogenic cells permits immediate grafts availability and possibility of stockpiling and preserving grafts for future use. Preparation of epithelial sheets suitable for grafting is also faster and ...

1991-01-01

436

Use of nuclear techniques in studies of uptake and metabolic fate of xenobiotics in plants

From diseased wild and cultured Oreochromis niloticus in Lower Egypt, 17 Aeromonas hydrophila isolates were recovered. The mortality was between 10% and 70% in among cultured fish. The course of the disease ran in an acute manner. For cultured fish, the disease outbreaks were found mainly in winter and for the wild Nile fish, mortalities were observed in late spring and summer. Additionally wild fish were affected with ectoparasites. The LD50 values of the isolates ranged between 10(3) and 10(7). Isolates of high virulence were resistant to 1 hr boiling and to the bactericidal effect of fresh normal guinea pig serum. Moreover, they did not agglutinate in acriflavin. Only the virulent isolates could agglutinate tilapia erythrocytes. The above effects were reversed for avirulent isolates while moderately virulent isolates showed no consistency in their reactions. Tube agglutination test using O and WC antisera prepared ...

1989-03-01

437

Statistical optimization of biohydrogen production from sucrose by a co-culture of Clostridium acidisoli and Rhodobacter sphaeroides

The use of municipal sewage sludge as a fertilizer in agriculture is a convenient method of disposal. However, sludge is often contaminated with toxic organic compounds such as dioxins, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs), with implications for soil fertility and quality of crops for human and animal consumption. These compounds can be assimilated by intact plants or in-vitro cell-Culture systems. The amount of uptake depends on the plant species and on the physico-chemical conditions that influence, for example, molecular configuration; uptake rates are higher with low-molecular-weight and polar compounds. The xenobiotic can be converted to polar conjugates and hydroxylated metabolites that may also be toxic. In some cases, large amounts of the compound and/or its metabolic products are incorporated into non-extractable residues. The bound residues, especially those associated with carbohydrate fractions of the cell wall, ...

1997-10-01

438

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation

Statistically based experimental designs were applied to optimize the fermentation process parameters for hydrogen (H{sub 2}) production by co-culture of Clostridium acidisoli and Rhodobacter sphaeroides with sucrose as substrate. An initial screening using the Plackett-Burman design identified three factors that significantly influenced H{sub 2} yield: sucrose concentration, initial pH, and inoculum ratio. These factors were considered to have simultaneous and interdependent effects. A central composite design and response surface analysis were adopted to further investigate the mutual interactions among the factors and to identify the values that maximized H{sub 2} production. The optimal substrate concentration, initial pH, and inoculum ratio of C. acidisoli to R. sphaeroides were 11.43 g/L sucrose, 7.13, and 0.83, respectively. Using these optimal culture conditions, substrate conversion efficiency was determined as 10.16 mol H{sub 2}/mol ...

2010-05-15

439

British Library Electronic Table of Contents (United Kingdom)

Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24?h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western bl...

2008-01-01

440

Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn.

British Library Electronic Table of Contents (United Kingdom)

Callus cultures of Calophyllum inophyllum were established using seed, nodal/ internodal and leaf explants on WPM basal medium supplemented with indole-3-butyric acid (IBA), a-naphthalene acetic acid (NAA), picloram (4-amino-3,5,6-trichloropicolinic acid), and 6-benzylaminopurine (BAP) in different combinations and concentrations with the view to study the influence of hormones on callus induction and the pattern of expression of dipyranocoumarins including anti-HIV, non-nucleoside reverse transcriptase inhibitors inophyllum B and P in callus cultures. 96.01% seed explants, 87.50% nodal/internodal explants and 86.66% leaf explants were converted into calluses when inoculated on WPM supplemented with IBA 4.0mgl-1 along with BAP 1.0mgl-1, IBA 4.0mgl-1, and picloram 6.0mgl-1 along with BAP 2....

2007-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Optimization of culture conditions in CO2 fixation for succinic acid production using Actinobacillus succinogenes

British Library Electronic Table of Contents (United Kingdom)

The culture conditions in CO2 fixation by Actinobacillus succinogenes for succinic acid production were investigated by a model of available CO2 in a 3-l fermentor. The results from the model analysis showed that the available CO2 for succinic acid production in the fermentation broth is the sum of HCO3 ?, CO3 2?, and CO2 influenced by external culture conditions such as medium components, CO2 partial pressures, and temperature. The optimized conditions for CO2 supply in a 3-l fermentor were determined as follows: CO2 partial pressure and stirring speed were maintained at 0.1?MPa and 200?r?min?1, respectively, with a pH of 6.8 and a temperature of 37?C; 0.15?mol?l?1 NaHCO3 was added. Under the optimized conditions, a CO2 fixation rate of 0.57?g?l?1?h?1 was obtained, and a succinic acid con...

2011-01-01

442

Genotoxic effects of sunlight-activated waste waters

Examination of thermally polluted water for free living amoebae and testing for their possible pathogenic properties

Natural sunlight induces a genotoxic response in cultured CHO cells pre-treated with shale oil retort process water. Near ultraviolet light (NUV) component of the solar spectrum is the apparent radiation responsible for photoactivation. Cultured human skin fibroblasts are acutely sensitive to the genotoxic effects of photoactivated process water. The mutagenic potential of photoactivated process water in human cells is the same as that witnessed for an equivalent killing dose of the potent skin carcinogen FUV. DNA repair processes are involved in modulating genotoxic effects of this photo-induced process. The exact magnitude of the potential health-related and environmental risks resulting from photoactivation of retort process waters and other oil shale by-products is unassessed at this time. Our demonstration that a significant rate of mutation occurs in cultured human cells exposed to high dilutions of process waters and ...

1981-01-01

443

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay

Water and mud samples were collected from canals and rivers which were adjacent to outlets discharging warm water of 3 power plants in Berlin. Downstream samples from 1 bathing resort were also collected. Free living amoebae were isolated from 138 water and 69 mud samples. From these respectively 156 and 73 strains could be cultured and were administered intranasally to mice for pathogenicity tests. Two Acanthamoeba strains from water and 7 from mud could be reisolated from mouse brain and or lungs, although no pathological disorders could be observed. Five Naegleria strains were negative in mouse inoculation tests. Four Acanthamoeba strains which were positive in mice were cultured at + 45 degrees C; no cytopathogenic effects were observed in tissue cultures. Acanthamoeba infective for mice could also be isolated from samples at low water temperatures. Further investigations have to show, whether changes in virulence of ...

1982-05-01

444

Elimination of a new ampelovirus (GLRaV-Pr) and Grapevine rupestris stem pitting associated virus (GRSPaV) from two Vitis vinifera cultivars combining in vitro thermotherapy with shoot tip culture

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis lucida, hepatoma-derived cell cultures exposed to cadmium or ...

1994-08-01

445

British Library Electronic Table of Contents (United Kingdom)

A new virus species designated as Grapevine leafroll associated virus-Pr (GLRaV-Pr), which is classified in a distinct phylogenetic group of the genus Ampelovirus (Closteroviridae), was recently characterized from Greek grapevine cultivars. Elimination studies of GLRaV-Pr were carried out in two grapevine cultivars, 'Mantilaria' and 'Prevezaniko', co-infected with Grapevine rupestris stem pitting associated virus (GRSPaV, Flexiviridae). Both viruses were detected by nested RT-PCR assays. Virus elimination was achieved by combining in vitro thermotherapy with meristem ( 0.2mm) or shoot tip culture ( 0.5cm). The survival and regeneration rate of meristems was very low. On the other hand, high survival rates were observed in the cultured shoot tips accompanied with high elimination rates for ...

2009-01-01

446

Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad

British Library Electronic Table of Contents (United Kingdom)

Abstract The objective of this study was to determine the functional properties of cartilaginous tissues generated by porcine MSCs isolated from different tissue sources, and to compare these properties to those derived from chondrocytes (CCs). MSCs were isolated from bone marrow (BM) and infrapatellar fat pad (FP), while CCs were harvested from the articular surface of the femoro-patellar joint. Culture-expanded CCs and MSCs were encapsulated in agarose hydrogels and cultured in the presence of TGF3. Samples were analysed biomechanically, biochemically and histologically at days 0, 21 and 42. After 42 days in free swelling culture, mean GAG content was 1.50% w/w in CC-seeded constructs, compared to 0.95% w/w in FP- and 0.43% w/w in BM-seeded constructs. Total collagen accumulation was hig...

2011-01-01

447

Cellulolytic Enzymes Production via Solid-State Fermentation: Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate.

Biological conversion of synthesis gas. Limiting conditions/scale-up

We investigated the effect of pretreatment on the physicochemical characteristics-crystallinity, bed porosity, and volumetric specific surface of soybean hulls and production of cellulolytic enzymes in solid-state fermentation of Trichoderma reesei and Aspergillus oryzae cultures. Mild acid and alkali and steam pretreatments significantly increased crystallinity and bed porosity without significant change inholocellulosic composition of substrate. Crystalline and porous steam-pretreated soybean hulls inoculated with T. reesei culture had 4 filter paper units (FPU)/g-ds, 0.6?IU/g-ds ?-glucosidase, and 45?IU/g-ds endocellulase, whereas untreated hulls had 0.75?FPU/g-ds, 0.06?IU/g-ds ?-glucosidase, and 7.29?IU/g-ds endocellulase enzyme activities. In A. oryzae steam-pretreated soybean hulls had 47.10?IU/g-ds endocellulase compared to 30.82?IU/g-ds in untreated soybean hulls. Generalized linear statistical model fitted to enzyme activity data ...

2011-06-15

448

Biohydrogen production from desugared molasses (DM) using thermophilic mixed cultures immobilized on heat treated anaerobic sludge granules

The purpose of this research is to develop a technically and economically feasible process for biologically producing H(sub 2) from synthesis gas while, at the same time, removing harmful sulfur gas compounds. Six major tasks are being studied: 1. Culture development, where the best cultures are selected and conditions optimized for simultaneous hydrogen production and sulfur gas removal; 2. Mass transfer and kinetic studies in which equations necessary for process design are developed; 3. Bioreactor design studies, where the cultures chosen in Task 1 are utilized in continuous reaction vessels to demonstrate process feasibility and define operating conditions; 4. Evaluation of biological synthetic gas conversion under limiting conditions in preparation for industrial demonstration studies; 5. Process scale-up where laboratory data are scaled to larger-size units in preparation for process demonstration in a pilot-scale ...

1993-09-01

449

British Library Electronic Table of Contents (United Kingdom)

Hydrogen production from desugared molasses (DM) was investigated in both batch and continuous reactors using thermophilic mixed cultures enriched from digested manure by load shock (loading with DM concentration of 50.1 g-sugar/L) to suppress methanogens. H"2 gas, free of methane, was produced during batch cultivations, at different (DM) concentrations ranging from 1.5 g-sugars/L to 50.1 g-sugars/L. The highest yield of 237 ml-H"2/g-sugar was achieved during the DM batch fermentation at concentration of 2.1 g-sugars/L, whereafter the yield decreased with increasing DM concentration. The enriched hydrogen producing mixed culture achieved from the 16.7 g-sugars/L DM batch cultivation was immobilized on heat treated anaerobic sludge granules in an up-flow anaerobic sludge blanket (UASB) reac...

2011-01-01

450

6-Methyl-1,2,4-benzenetriol, a new intermediate in penicillic acid biosynthesis in Penicillium cyclopium

Variations in ventral root axon morphology and locomotor behavior components across different inbred strains of mice

Penicillic acid-negative mutants were obtained from a color mutant derived from Penicillium cyclopium NRRL 1888 through N-methyl-N'-nitro-N-nitrosoguanidine treatment. One mutant (SK2N6) accumulated 6-methyl-1,2,4-benzenetriol, which was not previously known to be a metabolite of P. cyclopium, in addition to orsellinic acid and orcinol. The radioactivity of [1-"1"4C]acetic acid was rapidly incorporated into 6-methyl-1,2,4-benzenetriol in a culture of P. cyclopium SK2N6. Moreover, the radioactivity of ["1"4C]6-methyl-1,2,4-benzenetriol was efficiently incorporated into penicillic acid in a culture of P. cyclopium NRRL 1888. These data indicate that 6-methyl-1,2,4-benzenetriol is a precursor for penicillic acid biosynthesis. The results on the addition of 1,4-dihydroxy-6-methoxy-2-methylbenzene, 6-methoxy-2-methylbenzoquinone (1,4), and 1-O-methylorcinol to a culture of P. cyclopium SK2N6 indicated that only the former two ...

451

British Library Electronic Table of Contents (United Kingdom)

Locomotion is a complex behavior affected by many different brain- and spinal cord systems, as well as by variations in the peripheral nervous system. Recently, we found increased gene expression for EphA4, a gene intricately involved in motor neuron development, between high-active parental strain C57BL/6J and the low-active chromosome substitution strain 1 (CSS1). CSS1 mice carry chromosome 1 from A/J mice in a C57BL/6J genetic background, allowing localization of quantitative trait loci (QTL) on chromosome 1. To find out whether differences in motor neuron anatomy, possibly related to the changes in EphA4 expression, are involved in the motor activity differences observed in these strains, motor performance in various behavioral paradigms and anatomical differences in the ventral roots ...

2009-01-01

452

Value of ultrasonography, Ct and MR imaging in the diagnosis of primary hyperparathyroidism

Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.).

Purpose: To evaluate the significance of preoperative localization of abnormal parathyroid glands to the surgical outcome in patients with primary hyperparathyroidism. Material and Methods: Thirty-nine patients with primary hyperparathyroidism were studied preoperatively with US (39 patients), CT (30 patients) and MR imaging (18 patients). The overall diagnostic accuracy for US was 87%, CT 66% and MR 94%. In patients with a single parathyroid adenoma US was the most cost-effective localization technique with a detection rate of 96%. CT had a lower detection rate (78%) but was of particular value for fairly large ectopic adenomas in the root of the neck. MR imaging was a good confirmatory test (93%). In patients with multiple gland disease (primary hyperplasia and multiple adenomas), no single localization study alone was sufficient. Combination of all 3 studies, however, alerted the physician to the presence of disease in more than one gland in 87% of these ...

1996-09-01

453

The first placebo-controlled trial of a special butterbur root extract for the prevention of migraine: reanalysis of efficacy criteria.

High salinity is one of the main factors limiting cotton growth and productivity. The genes that regulate salt stress in TM-1 upland cotton were monitored using microarray and real-time PCR (RT-PCR) with samples taken from roots. Microarray analysis showed that 1503 probe sets were up-regulated and 1490 probe sets were down-regulated in plants exposed for 3h to 100mM NaCl, and RT-PCR analysis validated 42 relevant/related genes. The distribution of enriched gene ontology terms showed such important processes as the response to water stress and pathways of hormone metabolism and signal transduction were induced by the NaCl treatment. Some key regulatory gene families involved in abiotic and biotic sources of stress such as WRKY, ERF, and JAZ were differentially expressed. Our transcriptome analysis might provide some useful insights into salt-mediated signal transduction pathways in cotton and offer a number of candidate genes as potential markers of tolerance to ...

2011-04-30

454

Recovery of {sup 15}N-urea in soil-plant system of tanzania grass pasture; Recuperacao de {sup 15}N-ureia no sistema solo-planta de pastagem de capim-Tanzania

This is an independent reanalysis of a randomised, placebo-controlled parallel-group study on the efficacy and tolerability of a special butterbur root extract (Petadolex) for the prophylaxis of migraine. The original protocol and analysis had a number of major shortcomings. In order to follow regulatory requirements, an independent reanalysis of the original data was performed. Following a 4-week baseline phase, 33 patients were randomised to treatment with two capsules 25 mg butterbur twice a day and 27 to placebo. The mean attack frequency per month decreased from 3.4 at baseline to 1.8 after 3 months (p = 0.0024) in the verum group and from 2.9 to 2.6 in the placebo group (n.s.). The responder rate (improvement of migraine frequency > or =50%) was 45% in the verum group and 15% in the placebo group. Butterbur was well tolerated. This small trial indicates that butterbur may be effective in the prophylaxis of migraine. PMID:14752215

2004-01-28

455

Recovery of "1"5N-urea in soil-plant system of tanzania grass pasture

The economic attractiveness and negative environmental impact of nitrogen (N) fertilization in pastures depend on the N use efficiency in the soil-plant system. However, the recovery of urea-{sup 15}N by Panicum maximum cv. Tanzania pastures, one of the most widely used forage species in intensified pastoral systems, is still unknown. This experiment was conducted in a randomized complete block design with four treatments (0, 40, 80 and 120 kg ha-1 of N-urea) and three replications, to determine the recovery of {sup 15}N urea by Tanzania grass. Forage production, total N content and N yield were not affected by fertilization (p > 0.05), reflecting the high losses of applied N under the experimental conditions. The recovery of {sup 15}N urea (% of applied N) in forage and roots was not affected by fertilization levels (p > 0.05), but decreased exponentially in the soil and soil-plant system (p < 0.05) with increasing urea doses. The amount of ...

2009-01-15

456

Phenolics from Glycyrrhiza glabra roots and their PPAR-g ligand-binding activity

The economic attractiveness and negative environmental impact of nitrogen (N) fertilization in pastures depend on the N use efficiency in the soil-plant system. However, the recovery of urea-"1"5N by Panicum maximum cv. Tanzania pastures, one of the most widely used forage species in intensified pastoral systems, is still unknown. This experiment was conducted in a randomized complete block design with four treatments (0, 40, 80 and 120 kg ha-1 of N-urea) and three replications, to determine the recovery of "1"5N urea by Tanzania grass. Forage production, total N content and N yield were not affected by fertilization (p > 0.05), reflecting the high losses of applied N under the experimental conditions. The recovery of "1"5N urea (% of applied N) in forage and roots was not affected by fertilization levels (p > 0.05), but decreased exponentially in the soil and soil-plant system (p < 0.05) with increasing urea doses. The amount of "1"5N (kg ha"-"1) in ...

457

British Library Electronic Table of Contents (United Kingdom)

Bioassay-guided fractionation of the EtOH extract of licorice (Glycyrrhiza glabra roots), using a GAL-4-PPAR-g chimera assay method, resulted in the isolation of 39 phenolics, including 10 new compounds (1-10). The structures of the new compounds were determined by analysis of their spectroscopic data. Among the isolated compounds, 5prime-formylglabridin (5), (2R,3R)-3,4prime,7-trihydroxy-3prime-prenylflavane (7), echinatin, (3R)-2prime,3prime,7-trihydroxy-4prime-methoxyisoflavan, kanzonol X, kanzonol W, shinpterocarpin, licoflavanone A, glabrol, shinflavanone, gancaonin L, and glabrone all exhibited significant PPAR-g ligand-binding activity. The activity of these compounds at a sample concentration of 10mg/mL was three times more potent than that of 0.5mM troglitazone.

2010-01-01

458

Nearly metastable rhombohedral phases of bcc metals

Influence of sewage sludge compost applications on uptake of element by cultivated crops in a brown forest soil. Measurement by neutron activation analysis

The energy E(c/a) for a bcc element stretched along its [001] axis (the Bain path) has a minimum at c/a=1, a maximum at c/a=#sq root#(2), and an elastically unstable local minimum at c/a>#sq root#(2). An alternative path connecting the bcc and fcc structures is the rhombohedral lattice. The primitive lattice has R3m symmetry, with the angle #alpha# changing from 109.4 deg. (bcc), to 90 deg. (simple cubic), to 60 deg. (fcc). We study this path for the non-magnetic bcc transition metals (V, Nb, Mo, Ta, and W) using both all-electron linearized augmented plane wave and projector augmented wave VASP codes. Except for Ta, the energy E(#alpha#) has a local maximum at #alpha#=60 deg., with local minima near 55 deg. and 70 deg., the latter having lower energy, suggesting the possibility of a metastable rhombohedral state for these materials. We first examine the elastic stability of the 70 deg. minimum structure, and determine that only W is ...

2008-02-01

459

Fuji computed radiography (FCR) for the diagnosis of spinal disorders

A field study was conducted to investigate the absorption of various elements into oats and carrots cultivated in brown forest soil after three years' applications of chemical fertilizer and two types of sewage sludge compost mixed with sawdust (SD compost) or rice husk (RH compost). The results obtained in this study are summarized as follows. 1) The application of SD compost led to a significant increase on the concentrations of Mn, Zn, Ag and Ba in oat root, of Zn and Br in oat shoot, of Cl and Zn in oat ears, of Mg, Sc, Mn, Zn, Br, Ba and La in carrot peel, of Mn, Fe, Co and Zn in carrot edible portion and of Na, Sc, Mn, Fe, Co and Sm in carrot shoot. 2) The application of RH compost increased the concentrations of Mn, Zn, and Ag in oat root, of K, Cr, Mn, Zn and Br in oat shoot, of Zn and Br in oat ears, of Mg, Mn and Br in carrot peel, of Cl, Mn, Zn and Br in carrot edible portion and of Na, Mn, Zn, Br and Sm in carrot shoot. (author)

2006-03-01

460

Evaluation of antioxidant and anti-atherogenic properties of Glycyrrhiza glabra root using in vitro models.

Since April, 1985, we have, in co-operation with the Fuji Film Co., Ltd., used Fuji Computed Radiography (FCR) in the diagnosis of spinal disorders. FCR is a new computed radiographic system which uses an energy-storage phosphorus panel called an ''Imaging Plate'' as an image sensor. The ''Imaging Plate'' can be used to obtain radiographs in exactly the same way as the screen-film combination used in conventional radiography; X-rays are exposed on the ''Imaging Plate'' instead of X-ray film in the conventional fashion, and then the ''Imaging Plate'' is calculated. The processed digital data from the scans is transformed into a picture by means of digital-to-analogue conversion. The pictures are always clear and beautiful. Plain films of the spine taken by FCR are even clearer, even in the cervicothoracic region, where it is usually difficult to obtain clear cervicothoracic films in conventional radiography. We can obtain much precise information about the spinal posterior osteophytes ...

1987-01-01

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461

Effects of an acute dose of gamma radiation exposure on stem diameter growth, carbon gain, and biomass partitioning in Helianthus annuus

The aim of present study was to evaluate antioxidant property of Glycyrrhiza glabra root extracts using in vitro models. The dose-dependent aqueous and ethanolic extracts demonstrated the scavenging activity against nitric oxide (concentration that caused 50% inhibition of nitric oxide radicals [IC(50)]=72 and 62.1 microg/ml, respectively), superoxide (IC(50)=64.2 and 38.4 microg/ml, respectively), hydroxyl (IC(50)=81.9 and 63 microg/ml, respectively), DPPH (IC(50)=43.6 and 28.3 microg/ml, respectively) and ABTS(*+) (IC(50)=77.3 and 57.2 microg/ml, respectively) radicals. Further, both extracts showed strong reducing power and iron-chelating capacities. In the Fe(2+)/ascorbate system, both extracts were found to inhibit mitochondrial fraction lipid peroxidation. In copper-catalyzed human serum and low-density lipoprotein oxidation models, both extracts significantly (P<0.05) lengthened the lag phase along with a decline in the oxidation rate, conjugated dienes, ...

2009-04-22

462

Effect of gamma irradiation on the antimicrobial and free radical scavenging activities of Glycyrrhiza glabra root

Nineteen-day-old dwarf sunflower plants (Helianthus annuus, variety NK894) received a variable dose (0-40 Gy) from a cobalt-60 gamma source. A very sensitive stem monitoring device, developed at Battelle's Pacific Northwest Laboratories, Richland, Washington was used to measure real-time changes in stem diameter. Exposure of plants caused a significant reduction in stem growth and root biomass. Doses as low as 5 Gy resulted in a significant increase in leaf density, suggesting that nonreversible morphological growth changes could be induced by very low doses of radiation. Carbohydrate analysis of 40-Gy irradiated plants demonstrated significantly more starch content in leaves and significantly less starch content in stems 18 days after exposure than did control plants. In contrast, the carbohydrate content in roots of 40-Gy irradiated plants were not significantly different from unirradiated plants 18 days after exposure. These results ...

1988-05-25

463

Effect of external pH on the cytoplasmic and vacuolar pHs in Mung bean root-tip cells. A T P nuclear magnetic resonance study

The efficacy of gamma irradiation as a method of decontamination for food and herbal materials is well established. In the present study, Glycyrrhiza glabra roots were irradiated at doses 5, 10, 15, 20 and 25 kGy in a cobalt-60 irradiator. The irradiated and un-irradiated control samples were evaluated for phenolic contents, antimicrobial activities and DPPH scavenging properties. The result of the present study showed that radiation treatment up to 20 kGy does not affect the antifungal and antibacterial activity of the plant. While sample irradiated at 25 kGy does showed changes in the antibacterial activity against some selected pathogens. No significant differences in the phenolic contents were observed for control and samples irradiated at 5, 10 and 15 kGy radiation doses. However, phenolic contents increased in samples treated with 20 and 25 kGy doses. The DPPH scavenging activity significantly (p<0.05) increased in all irradiated samples of the plant.

2010-04-01

464

Effect of external pH on the cytoplasmic and vacuolar pHs in Mung bean root-tip cells

The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the T P nuclear magnetic resonance (NMR) method. The T P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Psub(i) and vacuolar Psub(i). The cytoplasmic and vacuolar pHs could be determined by comparing the Psub(i) chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H -ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H -ATPase appears to have an important part in the regulation of the intracellular pH.

1984-12-01

465

Differences in p,p'-DDE bioaccumulation from compost and soil by the plants Cucurbita pepo and Cucurbita maxima and the earthworms Eisenia fetida and Lumbricus terrestris

The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the "3"1P nuclear magnetic resonance (NMR) method. The "3"1P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Psub(i) and vacuolar Psub(i). The cytoplasmic and vacuolar pHs could be determined by comparing the Psub(i) chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H"+-ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H"+-ATPase appears to have an important part in the regulation of the intracellular pH. (author).

466

Comparison of NH_4"+-N and NO_3"--N nutrition in hybrid and conventional rice at the late growth stage

Two plant species, Cucurbita pepo and Cucurbita maxima, and two earthworm species, Eisenia fetida and Lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-DDE contamination. Pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in C. pepo. Bioaccumulation by E. fetida was approximately 6- and 3-fold higher than that by L. terrestris in the soil and compost, respectively. For all species, p,p'-DDE uptake was significantly greater from soil than from compost; 7- to 8-fold higher for plant roots and 3- to 7-fold higher for worms. Abiotic desorption from soil was approximately twice that from the compost. When all the data are normalized for organic-carbon content of the media, the contaminant is more tightly bound by soil than compost. Although the risk associated with p,p'-DDE is higher in soil than compost, important mechanistic differences exist in contaminant binding to organic ...

2007-07-01

467

Biological control of take-all in wheat by endophytic Bacillus subtilis E1R-j and potential mode of action

The difference of NH_4"+-N/NO_3"--N nutrition between hybrid and conventional rice varieties at the late growth stage was studied by using "1"5N-tracer technique. The results showed that the nitrate fertilizer utilization efficiency by the hybrid rice after anthesis was 7.8% higher than that by the ordinary rice variety, and the nitrate fertilizer recovery fraction by the former was 13.2% greater than that by the latter. The varietal difference in NO_3"--N uptake and utilization was almost twice as that in NH_4"+-N. It was also showed that "1"5N distribution in ear of the hybrid rice was about 20% greater than that of the conventional variety, but there were no obvious differences between NO_3"--N and NH_4"+-N. However, "1"5N distribution in the lower node leaves and root was found to be significant higher with NO_3"--N than that with NH_4"+-N in both rice varieties, particularly in the hybrid rice. Compared with NH_4"+-N, NO_3"--N top-dressing before anthesis was ...

468

British Library Electronic Table of Contents (United Kingdom)

The bacterial strain E1R-j, isolated as an endophyte from wheat roots, exhibited high antifungal activity to Gaeumannomyces graminis var. tritici (Ggt). Strain E1R-j was identified as Bacillus subtilis based on morphological, physiological and biochemical methods as well as on 16S rDNA analysis. This strain inhibited mycelium growth in vitro of numerous plant pathogenic fungi, especially of Ggt, Coniothyrium diplodiella, Phomopsis sp. and Sclerotinia sclerotiorum. In greenhouse experiments, soil drenches with cell densities of 106, 109 and 1012CFU ml-1 E1R-j reduced significantly take-all disease, caused by Ggt, in wheat seedling by 62.6%, 68.6% and 70.7%, respectively, compared to the inoculated control, 4weeks after sowing. Growth parameters such as lengths and fresh weights of roots and...

2009-01-01

469

BARBOX: An alternative system concept for floating production, storage, and offloading

Aspects of the development of mechanical superchargers; Aspekte der Entwicklung mechanischer Lader

This paper describes the Brown and Root`s BARBOX, an alternative, purpose-built, deep-draft monohull system for floating production, storage, and offloading (FPSO). This alternative system concept has been designed for worldwide applications including the North Sea and the Gulf of Mexico environments. The stiffened flat plate construction of the BARBOX gives it a low capital investment cost relative to minimum water plane hull types (e.g., semi-submersible, SPAR, TLP) and ship-shaped FPSO. Dynamic motion comparison between the BARBOX and the ship-shaped FPSO indicates that the BARBOX`s motion characteristics are similar or better than those of comparable ship-shaped FPSO configurations. This paper also considers construction of the BARBOX hull in the Gulf of Mexico region. A recent study indicated that the Gulf coast fabrication yards were technically capable to fabricate the BARBOX hull and they could be cost competitive against worldwide shipyards.

1997-07-01

470

Zebrafish embryo extracts promote sphere-forming abilities of human melanoma cell line

Internal combustion engines with mechanically driven superchargers are known for good performance at low engine speeds and fast response times under fluctuating loads. Application engineers can choose between superchargers with and without internal compression - the familiar roots (blower) type or true compressors - for supercharging an internal combustion engine. This article by DaimlerChrysler describes two options for improving the partial load behaviour of mechanically supercharged internal combustion engines with compressors. (orig.) [German] Die Aufladung mit mechanisch angetriebenen Verdraengerladern zeichnet sich durch guten Druckaufbau bei niedrigen Drehzahlen und gute Dynamik bei Lastwechseln aus. Fuer die Applikation am Verbrennungsmotor koennen Maschinen ohne innere Verdichtung - wie die bekannten Roots-Geblaese - oder Maschinen mit innerer Verdichtung - Kompressoren im eigentlichen Sinne - zum Einsatz kommen. In diesem Beitrag von ...

2001-11-01

471

British Library Electronic Table of Contents (United Kingdom)

Sphere-forming abilities in culture condition are considered a hallmark of cancer stem-like cells, which represents tumor cell invasiveness and stem-like characteristics. We aimed to show that the sphere-forming subpopulation of human malignant melanoma cell line WM-266-4 acts differently to zebrafish embryo extracts compared with their bulk counterpart. Spheres were maintained in neural stem cell culture conditions. The embryos of zebrafish at specific developmental stages were collected and the extracts were purified under 100 kDa. Spheres were treated with embyo extracts and proliferation assay and immunocytochemistry were conducted. Spheroid cells expressed nestin and epidermal growth factor receptor (EGFR) but not melanoma antigen recognized by T-cells (MART)1, indicating their stem-l...

2009-01-01

472

X-ray fluorescence in research on Czech cultural monuments

X-ray fluorescence in research on Czech cultural monuments

Radionuclide X-ray fluorescence analysis (RXRFA), as a non-destructive method, relatively simple and allowing measurements to be carried out in situ, is an excellent tool in research on various objects of art. A range of artefacts have been investigated by our laboratory, in part for the purposes of history of art and in part as a basis for restoration works - medieval frescoes in some Czech castles and churches, metal sculptures and objects of applied art, paints and inks of old manuscripts, paintings. Some of these are among the most valuable monuments in the Czech cultural heritage. The contribution of the results of the tests to the investigation of their 'life story' and, in some cases, to their conservation, is not negligible. Later additions and repairs can be recognised, and materials and technologies that are close to their historic counterparts can be used in restoration work.

2004-01-01

473

Use of flow cytometry to compare the antimicrobial efficacy of silver-containing wound dressings against planktonic Staphylococcus aureus and Pseudomonas aeruginosa

Radionuclide X-ray fluorescence analysis (RXRFA), as a non-destructive method, relatively simple and allowing measurements to be carried out in situ, is an excellent tool in research on various objects of art. A range of artefacts have been investigated by our laboratory, in part for the purposes of history of art and in part as a basis for restoration works - medieval frescoes in some Czech castles and churches, metal sculptures and objects of applied art, paints and inks of old manuscripts, paintings. Some of these are among the most valuable monuments in the Czech cultural heritage. The contribution of the results of the tests to the investigation of their 'life story' and, in some cases, to their conservation, is not negligible. Later additions and repairs can be recognised, and materials and technologies that are close to their historic counterparts can be used in restoration work.

2004-01-01

474

British Library Electronic Table of Contents (United Kingdom)

Abstract Silver-impregnated wound dressings continue to be routinely used for the management of infected wounds, or wounds that are at risk of becoming infected. The ability of antimicrobials that have been incorporated into wound dressings to kill microorganisms within the dressing requires appropriate evaluation using in vitro models. In vitro models that have been exploited for this purpose have included the corrected zone of inhibition and the log reduction assay. However, these and other related culturable-based assays are purported to have poor correlation with the overall microbicidal barrier activity of an antimicrobial wound dressing. This is because culturable-based methods only retrospectively indicate bacterial cell death and do not take into account viable but nonculturable st...

2011-01-01

475

The Relevance of Art Education and the Education of the Nigerian Child: Implications for the Universal Basic Education Policy

Terror management among Taiwanese: Worldview defence or resigning to fate?

This article examines relevant government policy documents on education and culture and discovers that Nigerian education authorities do not "discriminate" against art and culture in its articulation of educational policies per se, but lack of administrative machinery or political will has resulted in the deprivation of the Nigerian child in the process of creative activity in early childhood development. The article argues that lack of creative ability in our educational products is perhaps a major setback in the nation's quest for industrial and technological development. In this article a new art curriculum for elementary schools is advocated as a means of engaging the young child in order to attain functional educational skills necessary in the world of work.

2008-10-01

476

British Library Electronic Table of Contents (United Kingdom)

Terror management theory (TMT) proposes that people who are reminded of their mortality should be motivated to defend their cultural worldview. Studies 1 and 2 examined whether the TMT worldview defence-buffering effect found in Western cultures could be generalized to Asians in Taiwan. No such effect was found in the present studies. This non-significant result was robust when either a stronger distraction task was used (study 1) or when a subliminal manipulation of mortality salience was utilized (study 2). A meta-analysis, including 24 TMT experiments in East Asia, was also conducted (study 3). The average effect size (d = 0.11, r = 0.055) of worldview defence among these experiments was not significantly different from zero. Study 4 found that mortality salience manipulation also did n...

2010-01-01

477

Responses of the toxic cyanobacterium Microcystis aeruginosa to iron and humic substances

British Library Electronic Table of Contents (United Kingdom)

Iron is an essential element to marine biota. Different types of dissolved organic matter (DOM), such as humic substances have impacts on the marine coastal waters iron chemistry.The aim of the study was to examine how the presence of humic substances (both aquatic and sedimentary) may affect iron bioavailability to the bloom-forming cyanobacterium Microcystis aeruginosa Kutzing incubated on standard and modified mineral BG-11 media. The final iron concentrations in the growth media ranged from 0.1 to 100mM.The results demonstrate that both the growth rate and the concentration of chlorophyll a in cultures of M. aeruginosa are limited by insufficient (a 15-fold as compared with the cultures exposed to iron only at the same concentration. Sedimentary humic acids in the presence of iron at a...

2007-01-01

478

Researching ethnic minority students in a Chinese context: mixed methods design for cross cultural understandings

British Library Electronic Table of Contents (United Kingdom)

Research on and with ethnic minority students is characterised by a growing international literature that privileges 'liberal multiculturalism' as a lens through which to understand their experiences, yet ethnic diversity is constructed and responded to in many societies that are not underpinned by liberal democratic values. In this paper we use Joppke's binary to show how researching ethnic minority students outside of a liberal democratic framework requires methodologies that enable researchers to see beyond the invisibility that is often attached to ethnic minorities. We show how invisibility (i.e. antidiscrimination) can also be further culturally constructed, confounding even more any broader multicultural project. We canvass four broad areas including the role of legally binding legi...

2011-01-01

479

Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran

British Library Electronic Table of Contents (United Kingdom)

This study investigated the occurrence of free-living amoebae (FLA) in immunodeficiency wards of hospitals in Tehran, Iran. A total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), HIV, leukemia and oncology patients of five university hospitals were collected and examined for the presence of FLA using culturing and molecular approaches. Based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of Acanthamoeba, vahlkampfiid amoebae and Hartmannella. Out of 70 samples, 37 (52.9%) were positive for FLA. Acanthamoeba belonged to the T4 genotype was the most prevalent isolate. Presence of the T4 genotype on medical instruments, including an oxygen mask in an isolation room of an immunodeficiency pediat...

2011-01-01

480

Potential Protection of Green Tea Polyphenols Against 1800?MHz Electromagnetic Radiation-Induced Injury on Rat Cortical Neurons

British Library Electronic Table of Contents (United Kingdom)

Radiofrequency electromagnetic fields (EMF) are harmful to public health, but the certain anti-irradiation mechanism is not clear yet. The present study was performed to investigate the possible protective effects of green tea polyphenols against electromagnetic radiation-induced injury in the cultured rat cortical neurons. In this study, green tea polyphenols were used in the cultured cortical neurons exposed to 1800?MHz EMFs by the mobile phone. We found that the mobile phone irradiation for 24?h induced marked neuronal cell death in the MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide) and TUNEL (TdT mediated biotin-dUTP nicked-end labeling) assay, and protective effects of green tea polyphenols on the injured cortical neurons were demonstrated by testing the content ...

2011-01-01

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481

Photodynamic therapy against cyanobacteria

British Library Electronic Table of Contents (United Kingdom)

This study explores the use of photosensitizers and reactive oxygen species (ROS) to limit growth of cyanobacteria. We chose 12 phthalocyanines, tetraphenol porphyrine, and methylene blue as compounds producing singlet oxygen. Hydrogen peroxide was chosen as another source of ROS. These compounds were tested using algal toxicity tests in microplates on three cultures of green algae (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, and Chlorella kessleri) and on three cultures of cyanobacteria (Synechococcus nidulans, Microcystis incerta, and Anabaena sp.). Results indicate that photosensitizers and singlet oxygen could be highly toxic for some selected phytoplankton species. Green alga Scenedesmus quadricauda was highly sensitive (EC50 = 0.07 mg/L) to compounds producing singlet o...

2007-01-01

482

Pectinolytic yeast isolates for cold-active polygalacturonase production

British Library Electronic Table of Contents (United Kingdom)

Pectin rich cold stored spoiled fruits, vegetables and cold soils were screened and different pectinolytic isolates were obtained by enrichment culturing and ruthenium red plate assay. Among the primary isolates 10-15% were yeast isolates. Six isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase (PGU) production at room temperature (25 degrees C) and at 5 degrees C. One isolate identified as Saccharomyces sp. with highest polygalacturonase activity at 5 degrees C was used for enzyme production using raw fruit pectins as substrates. The isolate was identified by preliminary cultural, morphological and sugar fermentation tests. PGU production was high in raw pectin substrates like orange peel (21 U/ml), apple peel (20 U/ml ), mango peel (19 U/ml), ...

2011-01-01

483

Outcome of peripherally inserted central venous catheters in surgical and medical neonates

British Library Electronic Table of Contents (United Kingdom)

Background/Purpose: Peripherally inserted central venous catheters (PICCs) are commonly used for neonatal vascular access. The aim of this study was to look at PICC line complication rates and possible predictors of PICC infection in a neonatal intensive care unit. Method: This was a prospective study of 226 neonates who had PICCs on our neonatal intensive care unit between January 2006 and June 2009. Complete data was available on 218 neonates who had 294 PICC lines. Criteria for catheter-related sepsis was positive blood cultures (peripheral/central) and/or a positive catheter tip culture after removal in the presence of a clinical suspicion of line sepsis. Results: Of 218 neonates, 132 (169 lines) were medical, and 86 (125 lines) were surgical. Our PICC line infection rate was 17 infect...

2011-01-01

484

Nuclei with protrusions - tailed nuclei - and radiation cytogenetic markers in X-irradiated lymphocytes in vitro

Long-Term Follow-Up of Patients with Breast Cancer Transplanted with Autologous Ex Vivo Expanded Peripheral Blood Progenitor Cells

The results of an investigation into tailed nuclei (TN) and features of dose dependence on frequency of the binuclear cells with TN in human lymphocyte cultures by cytogenetic block method upon X-ray radiation dose are presented. The frequencies of metaphases with dicentric and ring chromosomes are investigated concurrent with TN formed upon completion of first postradiation mitosis. Frequency of the appearance of binuclear cells with nuclei having outgrowth into the cytoplasmic space and arises after first mitosis in human lymphocyte culture is linear-square dependent on the X-irradiation at doses from 0.0 to 4.0 Gy. Positive correlation between frequency of cells with tailed nuclei and frequency of metaphases of first mitosis having dicentric and rings was established. Apparently, formation such TN is connected with dicentric and rings

485

British Library Electronic Table of Contents (United Kingdom)

Ex vivo expanded peripheral blood progenitor cells (PBPC) have been shown to provide rapid neutrophil engraftment, and in some patients, to eliminate neutropenia after transplantation to support high-dose chemotherapy. However, the effect of expansion culture on stem cell content and potential loss of stem cells caused by induction of differentiation remains a concern. We have transplanted 21 patients with breast cancer with expanded autologous PBPC, with 11 patients receiving expanded PBPC as their sole hematopoietic cell source. In these studies, the CD34+ cells were selected and cultured for 10 days in defined media containing 100 ng/mL each of recombinant human stem cell factor (rhSCF), recombinant human granulocyte colony stimulating factor (rhG-CSF), and recombinant human megakaryocy...

2006-01-01

486

Investigating the biological and clinical significance of human dysbioses

British Library Electronic Table of Contents (United Kingdom)

Culture-independent microbiological technologies that interrogate complex microbial populations without prior axenic culture, coupled with high-throughput DNA sequencing, have revolutionized the scale, speed and economics of microbial ecological studies. Their application to the medical realm has led to a highly productive merger of clinical, experimental and environmental microbiology. The functional roles played by members of the human microbiota are being actively explored through experimental manipulation of animal model systems and studies of human populations. In concert, these studies have appreciably expanded our understanding of the composition and dynamics of human-associated microbial communities (microbiota). Of note, several human diseases have been linked to alterations in th...

2011-01-01

487

Historical Indigenous use of aquatic resources in Australia's Murray-Darling Basin, and its implications for river management

British Library Electronic Table of Contents (United Kingdom)

Summary Indigenous people demonstrably lived along rivers and around lakes and wetlands of Australia's Murray-Darling Basin in pre-European times. Waterways were, and continue to be, of major significance to the society and culture of Aboriginal peoples throughout Australia. Historically, they exploited most of the fauna - from mussels and crayfish, to fish and birds - and either ate, or used for other purposes, many species of aquatic plants. Such practices placed them in the role of environmental modifiers, a role played by all human groups from both past and present. They built sophisticated fish traps, cut gaps in river banks to allow fish to move on to floodplains, and there is evidence that they practised a form of fish culture by creating small impoundments in which small fish could...

2007-01-01

488

High-throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts

British Library Electronic Table of Contents (United Kingdom)

Optimization of nonviral gene delivery typically focuses on the design of particulate carriers that are endowed with desirable membrane targeting, internalization, and endosomal escape properties. Topographical control of cell transfectability, however, remains a largely unexplored parameter. Emerging literature has highlighted the influence of cell-topography interactions on modulation of many cell phenotypes, including protein expression and cytoskeletal behaviors implicated in endocytosis. Using high-throughput screening of primary human dermal fibroblasts cultured on a combinatorial library of microscale topographies, we have demonstrated an improvement in nonviral transfection efficiency for cells cultured on dense micropit patterns compared to smooth substrates, as verified with flow...

2011-01-01

489

Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers

British Library Electronic Table of Contents (United Kingdom)

Occurrence of genetic variants during micropropagation is occasionally encountered when the cultures are maintained in vitro for long period. Therefore, the micropropagated multiple shoots of Vanilla planifolia Andrews developed from axillary bud explants established 10 years ago were used to determine somaclonal variation using random amplified polymorphic DNA (RAPD) and intersimple sequence repeats markers (ISSR). One thousand micro-plants were established in soil of which 95 plantlets (consisting of four phenotypes) along with the mother plant were subjected to genetic analyses using RAPD and ISSR markers. Out of the 45 RAPD and 20 ISSR primers screened, 30 RAPD and 7 ISSR primers showed 317 clear, distinct and reproducible band classes resulting in a total of 30 115 bands. However, no ...

2007-01-01

490

Extracellular matrix of plant callus tissue visualized by ESEM and SEM

British Library Electronic Table of Contents (United Kingdom)

Actinidia deliciosa endosperm-derived callus culture is stable over a long period of culture. This system was used to investigate the ultrastructure of extracellular matrix occurring in morphogenic tissue. Specimens were prepared by different biological techniques (chemical fixation, liquid nitrogen fixation, glycerol substitution, critical-point drying, lyophilization) and observed by scanning electron microscopy (SEM). Fresh and wet samples were analyzed with the use of environmental scanning electron microscopy (ESEM). Extracellular matrix was observed on the surface of cell clusters as a membranous layer or reticulated network, shrunken or wrinkled, depending on the procedure. Generally, shrunken membranous layers with a globular appearance and fibrils were noted after critical-point d...

2010-01-01

491

Establishment of functional primary cultures of heart cells from the clam Ruditapes decussatus

British Library Electronic Table of Contents (United Kingdom)

Heart cells from the clam Ruditapes decussatus were routinely cultured with a high level of reproducibility in sea water based medium. Three cell types attached to the plastic after 2?days and could be maintained in vitro for at least 1?month: epithelial-like cells, round cells and fibroblastic cells. Fibroblastic cells were identified as functional cardiomyocytes due to their spontaneous beating, their ultrastructural characteristics and their reactivity with antibodies against sarcomeric ?-actinin, sarcomeric tropomyosin, myosin and troponin T-C. Patch clamp measurements allowed the identification of ionic currents characteristic of cardiomyocytes: a delayed potassium current (I K?slow) strongly suppressed (95%) by tetraethylammonium (1?mM), a fast inactivating potassium current (I K?fas...

2011-01-01

492

Endurance of cultured pearls irradiated with gamma-rays

Effects of cultivar and culture conditions on g-aminobutyric acid accumulation in germinated fava beans (Vicia faba L.)

Pearls change their color to grey by #gamma#-ray irradiation. Grey densities were determined from darkness of pearl nuclei and thickness of the pearl layers. The densities are independent of both diameters of nuclei and pearls. The fading rate increases with increasing storage temperature. The rate of fading for irradiated pearls is lower than that for natural blue pearls. Comparison of photomicrographs (x 12) for pearls irradiated and then stored at room temperature for about 6 years revealed that their surfaces are not substantially different from the surfaces of unirradiated cultured pearls, indicating that #gamma# irradiation hardly gives rise to deterioration. #gamma#-Ray irradiation is a technique for coloration of pearls. Irradiated blue pearls seem to have resistivity to fading and to deterioration of the surface, if pearls have been irradiated properly. (author).

493

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND: High levels of g-aminobutyric acid (GABA) accumulate in plant tissues under various stresses. GABA accumulation is also influenced by cultivar. This aim of this study was to select the most promising cultivar of fava bean for GABA accumulation and to optimise the culture conditions for GABA production in germinated fava beans by response surface methodology based on central composite design (CCD). RESULTS: GABA content and glutamate decarboxylase activity in germinated seeds of cultivar S2 were significantly higher than those in other cultivars (P < 0.05). A significant negative correlation (r = -0.765, P < 0.05) between germination percentage and 1000-kernel weight was observed. There was a linear relationship between GABA content and sprout length (R2 = 0.816). The regression...

2010-01-01

494

Do spatial effects appear at low dilution rate in chemostat?

British Library Electronic Table of Contents (United Kingdom)

The chemostat theory on two species competition has shown that the dilution rate where transition of dominance occurs - transition-dilution rate - is independent of limiting-nutrient concentration. However, we obtained the experimental data indicating that the transition-dilution rate changed with variations in limiting-ammonium concentrations, using the chemostat mixed-culture of the cyanobacterium Microcystis novacekii and the green alga Scenedesmus quadricauda. The transition-dilution rate was dependent on the concentration of limiting ammonium in the influx culture medium. We tried to simulate the experimental results. Though the dilution rate has been considered independent of nutrient concentration, we introduce the effective dilution rate that depends on nutrient concentration (ammo...

2009-01-01

495

Comparative Evaluation of Different Cell Lysis and Extraction Methods for Studying Benzo(a)pyrene Metabolism in HT-29 Colon Cancer Cell Cultures

British Library Electronic Table of Contents (United Kingdom)

Abstract Lysis and extraction of cells are essential sample processing steps for investigations pertaining to metabolism of xenobiotics in cell culture studies. Of particular importance to these procedures are maintaining high lysis efficiency and analyte integrity as they influence the qualitative and quantitative distribution of drug and toxicant metabolites in the intra- and extracellular milieus. In this study we have compared the efficiency of different procedures viz. homogenization, sonication, bead beating, and molecular grinding resin treatment for disruption of HT-29 colon cells exposed to benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) compound and a suspected colon carcinogen. Also, we have evaluated the efficiency of various procedures for extracting BaP parent c...

2011-01-01

496

Approaching the ?Resistant:? Exploring East Asian International Students? Perceptions of Therapy and Help-Seeking Behavior Before and After They Arrived in the United States

British Library Electronic Table of Contents (United Kingdom)

In this study we investigated cultural, familial, and individual beliefs that influenced international students? perceptions of therapy and help-seeking behaviors. Included were changes in perception or behavior before and after the students came to the US, and factors that influenced help-seeking behaviors. Six international students from East Asian countries where English is a foreign language, including Taiwan, Mainland China, Japan, and South Korea, were interviewed. We used semi-structured interviews and participant observations for collecting data. The findings indicated changes after students came to the US. Further discussions focus on encouraging and discouraging factors relative to seeing therapists, and culturally sensitive interventions for family therapy clinicians.

2011-01-01

497

An in Vitro Assessment of Interaction Between Grape Phylloxera and Indole Acetic Acid Treated Grape Plants Daktulosphaira Vitifolia (FITCH)

Allelopathic interactions between Prorocentrum micans and Skeletonema costatum or Karenia mikimotoi in laboratory cultures

the Life table of local strain of grape phylloxera was determined to evaluate the relationship between indole acetic acid (IAA) and phylloxera on our local variety Helwani. The study was carried out by applying in vitro dual culture system. The results showed that there was a great variation in mean developmental time, female longevity, number of laid eggs and egg distribution between all IAA concentrations and plant ages. Based on the tested biological parameters of phylloxera, (Helwani) would be unsuitable host for such destructive insect as it became older and when 2mg/1 of IAA was applied to in vitro culture media. (author)

498

British Library Electronic Table of Contents (United Kingdom)

Algal allelopathy is an ecological/physiological phenomenon that has focused attention on the interactions among algae and the production of algal toxins. We investigated the allelopathic interactions between the dinoflagellate genus Prorocentrum micans and diatom genus Skeletonema costatum and between P. micans and dinoflagellate genus Karenia mikimotoi using bi-algal cultures. Because the effects were species-specific and size-dependent, we evaluated the effect of different initial densities. At low densities of P. micans and high densities of S. costatum inoculated into the same medium, the growth of P. micans was weakly restrained, whereas the growth of S. costatum was significantly suppressed. S. costatum and K. mikimotoi were strongly inhibited by P. micans, in both the bi-algal cult...

2011-01-01

499

Alcohol fermentation in olive oil extraction effluents

5,8,11,14-Eicosatetraynoic acid-induced destruction of mitochondria in human prostate cells (PC-3).

Eight culture-collection yeast strains of various species and five newly isolated stains were tested for both growth in olive oil extraction effluents and fermentation of the sugars in the same media. The culture-collection yeast strains did not grow in an effluent containing 2.86% sugar (w/v), 8 g/litre phenolic substances, 4.58 g/litre titratable acidity and pH 4.96, whereas the newly isolated strains of Torulopsis sp. MK-1, Saccharomyces norbensis MC-1, S. oleaceus MC-2 and S. oleaginosus grew well and fermented the sugars. In the medium mentioned above, they produced alcohol in amounts of 1.63 to 1.38%, respectively. None of the yeasts grew in an olive oil extraction effluent vacuum-concentrated to over 13-14% of dry matter. The strain of T. sp. MK-1 showed a higher stability.

1989-01-01

500