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Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an Avena sativa Plasma Membrane Determinant 1

In whole-cell recording, the conductance of the plasma membrane of protoplasts isolated from mesophyll cells of leaves of oat (Avena sativa) was greater for inward than outward current....Full Text Available

1992-03-01

Phytochrome-controlled Hydrogen Ion Excretion by Avena Coleoptiles ¹

An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target...Full Text Available

1992-01-01

Western Blot Analysis of Cereal Grain Prolamins Using an Antibody to Carboxyl-Linked Indoleacetic Acid

A red light-induced, far red reversible stimulation of proton efflux from apical segments of etiolated Avena sativa L. cv. Victory coleoptiles was observed. The acidification...Full Text Available

1977-04-01

Use of Lipophilic Cations to Measure the Membrane Potential of Oat Leaf Protoplasts 1

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor,...Full Text Available

1991-08-01

The Metabolism of Oat Leaves during Senescence

Uptake of the lipophilic cation triphenylmethylphosphonium into mesophyll protoplasts of oat (Avena sativa L. cv. “Garry”) approaches equilibrium at 3 to 4 hours. The...Full Text Available

1978-12-01

Osmotic Shock Inhibits Auxin-stimulated Acidification and Growth ¹

When the detached first leaves of green or etiolated oat (Avena sativa cv. Victory) seedlings senesce in the dark, their oxygen consumption shows a large increase, beginning after 24...Full Text Available

1974-09-01

Kinetics of the Vacuolar H⁺-Pyrophosphatase ¹

Cells of oat coleoptiles (Avena sativa L. cv. “Garry”) have been osmotically shocked in order to observe the effect of alterations of the plasma membrane on some auxin...Full Text Available

1977-03-01

Osmoregulation in the Avena coleoptile: control of solute uptake in peeled sections

The responses of the vacuolar membrane (tonoplast) proton-pumping inorganic pyrophosphatase (H⁺-PPase) from oat (Avena sativa L.) roots to changes in Mg²⁺ and...Full Text Available

1992-12-01

Heavy metal concentrations in plants and different harvestable parts: A soil-plant equilibrium model

Peeled Avena sativa coleoptile sections have been used to study the control of solute uptake under conditions where the uptake is not limited by the cuticular barrier. In the presence of 2% sucrose, auxin enhances the rate at which the total osmotic solutes increase, but this appears to be a response to the increased growth rate, inasmuch as the auxin effect is eliminated when growth is inhibited osmotically. When sections are incubated in sucrose or in 10 millimolar NaCl, the osmotic concentration increases until a plateau is reached after 8 to 24 hours. Auxin has no effect on the initial rate of increase in osmotic concentration. This difference in steady-state osmotic concentration is, in part, a response to auxin itself, as it persists when auxin-induced growth is inhibited osmotically. The upper limit for osmotic concentration does not appear to be determined by the turgor pressure, inasmuch as a combination of sucrose and NaCl gave a ...

1982-01-01

Nodule and Leaf Nitrate Reductases and Nitrogen Fixation in Medicago sativa L. under Water Stress

A mathematical interaction model, validated by experimental results, was developed to modeling the metal uptake by plants and induced growth decrease, by knowing metal in soils. The model relates the dynamics of the uptake of metals from soil to plants. Also, two types of relationships are tested: total and available metal content. The model successfully fitted the experimental data and made it possible to predict the threshold values of total mortality with a satisfactory approach. Data are taken from soils treated with Cd and Ni for ryegrass (Lolium perenne, L.) and oats (Avena sativa L.), respectively. Concentrations are measured in the aboveground biomass of plants. In the latter case, the concentration of metals in different parts of the plants (tillering, shooting and earing) is also modeled. At low concentrations, the effects of metals are moderate, and the dynamics appear to be linear. However, increasing concentrations show nonlinear ...

2010-08-01

Nitrogen Fixation, Nodule Development, and Vegetative Regrowth of Alfalfa (Medicago sativa L.) following Harvest ¹

The effect of water stress on patterns of nitrate reductase activity in the leaves and nodules and on nitrogen fixation were investigated in Medicago sativa L. plants watered 1 week...Full Text Available

1982-02-01

Genetic Diversity Assessment of Rarely Cultivated Traditional Indica Rice (Oryza sativa L.) Varieties

Nitrogenase-dependent acetylene reduction, nodule function, and nodule regrowth were studied during vegetative regrowth of harvested (detopped) alfalfa (Medicago sativa L.) seedlings...Full Text Available

1979-07-01

Detection of Norspermidine and Norspermine in Medicago sativa L. (Alfalfa) ¹

Random amplified polymorphic DNA fingerprinting was performed to assess the genetic diversity among rarely cultivated traditional indica rice (Oryza sativa ...Full Text Available

Variation on Fatty Acid Profile Including ${\\gamma}-Linolenic$ acid among Hemp (Cannabis sativa L.) Accessions

Shoot meristem tissues of alfalfa, Medicago sativa L., were found by high performance liquid chromatography analyses to contain the uncommon polyamines, norspermidine and norspermine....Full Text Available

1989-02-01

KoreaScience (Korea)

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2005-08-01

A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity

KoreaScience (Korea)

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Phytochrome-mediated Electric Potential Changes in Oat Seedlings ¹

Excessive TV viewing and cardiovascular disease risk factors in adolescents. The AVENA cross-sectional study

Brief exposures to red light induce far red-reversible changes of 5 to 10 millivolts magnitude in the upper 1 centimeter of etiolated Avena coleoptiles. The changes begin within 15...Full Text Available

1972-12-01

Symbiotic regulation of plant growth, development and reproduction

BackgroundExcessive television (TV) viewing might play an important role in the development of cardiovascular disease (CVD). The aim of this study was to examine the independent...Full Text Available

Thymoquinone and cisplatin as a therapeutic combination in lung cancer: In vitro and in vivo

The growth and development of rice (Oryzae sativa) seedlings was shown to be regulated epigenetically by a fungal endophyte. In contrast to un-inoculated (nonsymbiotic) plants, endophyte...Full Text Available

2009-03-01

Structural Characterization and Expression Analysis of the SERK/SERL Gene Family in Rice (Oryza sativa)

BackgroundThymoquinone (TQ) is a compound extracted from Black Caraway seeds of Nigella Sativa and is active against various cancers. Cisplatin (CDDP) is the most...Full Text Available

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Proline Betaine Accumulation and Metabolism in Alfalfa Plants under Sodium Chloride Stress. Exploring Its Compartmentalization in Nodules1

Somatic embryogenesis (SE) is the developmental restructuring of somatic cells towards the embryogenic pathway and forms the basis of cellular totipotency in angiosperms. With the availability of full-length...Full Text Available

2009-01-01

Levels and Patterns of Nucleotide Variation in Domestication QTL Regions on Rice Chromosome 3 Suggest Lineage-Specific Selection

The osmoprotectant Pro betaine is the main betaine identified in alfalfa (Medicago sativa). We have investigated the long-term responses of nodulated alfalfa plants to salt stress,...Full Text Available

2004-07-01

Development of a novel data mining tool to find cis-elements in rice gene promoter regions

Oryza sativa or Asian cultivated rice is one of the major cereal grass species domesticated for human food use during the Neolithic. Domestication of this species from the wild grass...Full Text Available

Chromosome location of Oryza sativa recombination linkage groups.

BackgroundInformation on more than 35 000 full-length Oryza sativa cDNAs, together with associated microarray gene expression data collected under various treatment...Full Text Available

Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant¹^[W]^[OA]

In situ hybridization, a powerful tool for the molecular cytogeneticist, can be used to physically map repetitive, low-copy, and unique DNA sequences in plant chromosomes. With the availability of a...Full Text Available

1992-09-15

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation¹^[W]^[OA]

We characterized the absorption and short-term translocation of cadmium (Cd) in rice (Oryza sativa ‘Nipponbare’) quantitatively using serial images observed with a positron-emitting...Full Text Available

2010-04-01

Load-carrying capabilities of refractory alloys for space reactor power applications

Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in storage organelles in the endosperm during seed maturation as a nitrogen source for germinating seedlings....Full Text Available

2010-12-01

Load-carrying capabilities of refractory alloys for space reactor power applications

To achieve sufficient thermodynamic efficiency, space nuclear power systems must operate above 1000"0C. A quantitative evaluation of the existing mechanical properties data for the refractory alloys relevant to space nuclear power systems design lifetimes up to seven years at temperatures up to 1400"0C is being conducted. The most important properties for space nuclear power systems are long-term high-temperature (>1000"0C) creep strength and ductility, low-temperature (<400"0C) fracture toughness [including ductile-to-brittle transition temperature, (DBTT)], and ductility at high strain rates; of special concern are the above properties for weldments of refractory alloys, composition, applied stress, test temperature, test environment (e.g., vacuum, lithium), and thermomechanical treatment (TMT) history. Currently being evaluated are, in order of ascending mp, selected alloys of niobium (e.g., Nb-1% Zr, Nb-1% Zr-0.1% C), molybdenum (e.g., Mo-13% Re), tantalum (e.g., ASTAR-811C), ...

1985-01-14

Characterization of the mannan synthase promoter from guar (Cyamopsis tetragonoloba)

To achieve sufficient thermodynamic efficiency, space nuclear power systems must operate above 1000/sup 0/C. A quantitative evaluation of the existing mechanical properties data for the refractory alloys relevant to space nuclear power systems design lifetimes up to seven years at temperatures up to 1400/sup 0/C is being conducted. The most important properties for space nuclear power systems are long-term high-temperature (>1000/sup 0/C) creep strength and ductility, low-temperature (<400/sup 0/C) fracture toughness (including ductile-to-brittle transition temperature, (DBTT)), and ductility at high strain rates; of special concern are the above properties for weldments of refractory alloys, composition, applied stress, test temperature, test environment (e.g., vacuum, lithium), and thermomechanical treatment (TMT) history. Currently being evaluated are, in order of ascending mp, selected alloys of niobium (e.g., Nb-1% Zr, Nb-1% Zr-0.1% C), molybdenum (e.g., Mo-13% Re), tantalum ...

1985-01-01

British Library Electronic Table of Contents (United Kingdom)

Guar seed gum, consisting primarily of a high molecular weight galactomannan, is the most cost effective natural thickener, having broad applications in the food, cosmetics, paper, pharmaceutical and petroleum industries. The properties of the polymer can potentially be enhanced by genetic modification. Development of suitable endosperm-specific promoters for use in guar is desirable for metabolic engineering of the seed gum. A ~1.6?kb guar mannan synthase (MS) promoter region has been isolated. The MS promoter sequence was fused with the GUS reporter gene and overexpressed in the heterologous species alfalfa (Medicago sativa). The potential strength and specificity of the MS promoter was compared with those of the constitutive 35S promoter and the seed specific ?-phaseolin promoter. Quant...

2011-01-01

Sucrose phosphate synthase activity in rice grown at elevated CO sub 2 and temperature

On the formation and extent of uptake of silver nanoparticles by live plants

Rice (Oryza sativa L.) was grown at 330 and 660 {mu}L CO{sub 2} L{sup {minus}1} and at 40/33/37, 34/27/31, and 28/21/25{degree}C day/night/paddy water temperatures respectively. Sucrose phosphate synthase (SPS) activity was measured at saturating substrate concentrations at 59 days after planting. SPS activity increased 2 and 3 fold with increasing CO{sub 2} at 28 and 34{degree}C air temperatures respectively. At 40{degree}C SPS activity decreased by 37% at elevated CO{sub 2} and most plants failed to reach maturity. Similar responses were found in leaf samples taken in the dark. These results indicate that SPS, an enzyme involved in the regulation of C partitioning in leaves, increases in activity at elevated CO{sub 2}. This is in contrast to previous results with soybean. The changes in SPS activity will also be discussed in relation to leaf starch/sucrose ratios.

1990-05-01

Biological and chemical evaluation of sewage water pollution in the Rietvlei nature reserve wetland area, South Africa

In this work we investigate the limits of uptake of metallic silver by two common metallophytes, Brassica juncea (BJ) and Medicago sativa (MS) and assess the form and distribution of the metal once sequestered by the plants. BJ accumulated up to 12.4 wt.% silver when exposed to an aqueous substrate containing 1,000 ppm AgNO{sub 3} for 72 h, however silver uptake was largely independent of exposure time and substrate silver concentration. MS accumulated up to 13.6 wt.% silver when exposed to an aqueous substrate containing 10,000 ppm AgNO{sub 3} for 24 h. In contrast to BJ there was a general trend for MS showing an increase in metal uptake with a corresponding increase in the substrate metal concentration and exposure time. In both cases the silver was stored as discrete nanoparticles, with a mean size of {approx}50 nm. According to the hyperaccumulation definition of Brooks et al. (Brooks RR, Chambers MF, Nicks LJ, Robinson BH (1998) Phytomining. Trends Plant Sci ...

2008-04-15

Primary study on lesion mimic mutants of rice (oryza sativa L.)

Macroinvertebrate communities in Rietvlei nature reserve wetland area and their relationship with water quality were studied with the aim to evaluate their use as potential indicators of pollution. Sampling locations were selected to include outlets from swage effluent, agricultural and informal residential runoff. A large increase in nutrient concentrations was observed downstream from discharged treated sewage with an associated decrease in species richness. Bioassays performed included: Daphnia magna, Hydra attenuate, Lactuca sativa, Allium cepa and Pyxicephalus adspersus. The highest percentage of lethality response to a screen (100% concentration) of sampled wetland water by test specimens were observed at the point source input of the Hartbeespoort treated sewage plant. Data generated from the AUSRIVAS method and multitrophic level bioassays revealed the deterioration of the wetland possibly due to factors such as increasing urbanization, industrialization, ...

2008-11-01