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Caso probable de fiebre manchada ( Rickettsia felis) transmitida por pulgas / Probable case of flea-borne spotted fever ( Rickettsia felis )  

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Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Rickettsia felis es el agente etiológico de la fiebre manchada transmitida por pulgas, cuyo principal vector y reservorio es Ctenocephalides felis . Típicamente, la enfermedad se presenta como fiebre aguda asociada a cefalea, astenia, exantema máculo-papular generalizado y, en algunos casos, con esc [...] ara de inoculación. En los últimos años, R. felis ha venido adquiriendo un papel importante en la etiología del síndrome febril agudo, calificándola como una enfermedad emergente y subdiagnosticada. La inmunofluorescencia indirecta es actualmente el método diagnóstico de referencia. Sin embargo, esta técnica presenta limitaciones relacionadas con la reacción cruzada que existe entre las diferentes especies del género Rickettsia . En el presente reporte se describe el caso de un paciente de 16 años con síndrome febril agudo secundario a infección probable por R. felis . Abstract in english Rickettsia felis is the etiologic agent of flea-borne spotted fever, with Ctenocephalides felis as its main vector and reservoir. Typically, the disease presents as acute fever associated with headache, asthenia, generalized maculo-papular rash, and in some cases, an inoculation eschar. In recent ye [...] ars, R. felis has acquired an important role in the etiology of the acute febrile syndrome; it is indeed an emerging infectious disease, albeit underdiagnosed. Indirect immunofluorescence assay (IFA) is currently the reference diagnostic method. However, this technique has limitations related to the cross reactivity among different species of rickettsiae. Herein, we describe a case of a 16 year-old patient with an acute febrile syndrome secondary to probable infection with R. felis.

Álvaro A, Faccini-Martínez; Elkin G, Forero-Becerra; Jesús A, Cortés-Vecino; Luis J, Polo-Teran; Jorge H, Jácome; Jimmy J, Vargas; Gustavo, Valbuena; Marylin, Hidalgo.

2013-09-01

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Clinical and laboratorial evidence of Rickettsia felis infections in Latin America / Evidência clínica e laboratorial de infecções por Rickettsia felis na América Latina  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese Depois da descoberta e caracterização inicial da Rickettsia felis em 1992 por Azad e cols, e à descrição subseqüente do primeiro caso de infecção humana em 1994, houveram duas comunicações de rickettsioses causadas por Rickettsia felis na América Latina. A primeira foi feita por Zavala-Velazquez e c [...] ols em 2000 no México. Em 2001, Raoult e cols descreveram a ocorrência de dois casos humanos de rickettsiose por Rickettsia felis no Brasil. Na presente discussão, esses dois artigos foram comparados, e depois da descrição dos principais sinais e sintomas, conclui-se que outros estudos são necessários, com a participação de um maior número de pacientes, para se estabelecer a verdadeira freqüência dos sinais clínicos e sintomas presentes nas rickettsioses por Rickettsia felis. Abstract in english After the discovery and initial characterization of Rickettsia felis in 1992 by Azad and cols, and the subsequent first description of a human case of infection in 1994, there have been two communications of human rickettsiosis cases caused by Rickettsia felis in Latin America. The first one was pub [...] lished in 2000 by Zavala-Velazquez and cols in Mexico. In 2001 Raoult and cols described the occurrence of two human cases of Rickettsia felis rickettsiosis in Brazil. In the present discussion these two articles were compared and after the description of the principal signs and symptoms, it was concluded that more studies are needed with descriptions of a greater number of patients to establish the true frequency of the clinical signs and symptoms present in Rickettsia felis rickettsiosis.

Márcio Antônio Moreira, Galvão; Cláudio, Mafra; Chequer Buffe, Chamone; Simone Berger, Calic; Jorge E., Zavala-Velazquez; David Hughes, Walker.

2004-06-01

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TUBERCULOSIS GANGLIONAR RETROPERITONEAL Y MESENTERICA: CASO CLINICO  

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Full Text Available Se describen las características del compromiso ganglionar por Mycobacterium tuberculosis en un paciente con SIDA en quien se demuestra alteraciones de linfonódulos retroperitoneales y mesentéricos en tomografía computada. Se discute las diferencias con el compromiso secundario a infección por Mycobacterium avium intracellulare y además el diagnóstico diferencial con otras formas de compromiso ganglionarThe changes of retroperitoneal lymph nodes in CT in a patient with AIDS are described. Differences with the compromise with M avium-intracellulare are discussed an also the differential diagnosis with other lymph node pathologies

Rafael Martínez F

2004-01-01

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Vigilancia de la infección por Rickettsia sp. en capibaras (Hydrochoerus hydrochaeris) un modelo potencial de alerta epidemiológica en zonas endémicas / Surveillance of Rickettsia sp. infection in capybaras (Hydrochoerus hydrochaeris) a potential model of epidemiological alert in endemic areas  

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Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Introducción. Los capibaras o chigüiros (Hydrochoerus hydrochaeris) son huéspedes amplificadores de Rickettsia sp. Usualmente se encuentran parasitados por la garrapata Amblyomma cajennense, principal vector de rickettsiosis en Suramérica. Los capibaras pueden ser usados como potenciales centinelas [...] de la circulación de rickettsias. Objetivo. Detectar anticuerpos contra Rickettsia sp. del grupo de las fiebres manchadas en capibaras de una zona rural del municipio de Montería, departamento de Córdoba. Material y métodos. Se analizaron 36 sueros de capibaras de una zona rural de Montería (vereda San Jerónimo) en Córdoba. Para la detección de anticuerpos IgG se practicó inmunofluorescencia indirecta, que utilizó antígenos de la cepa Taiaçu de Rickettsia rickettsii de Brasil. Los sueros de los capibaras fueron diluidos 1:64. Se capturaron las garrapatas que se encontraban parasitando los capibaras y se clasificaron hasta su especie. Resultados. La seroprevalencia contra Rickettsia sp. del grupo de la fiebres manchadas encontrada fue de 22 % (8 capibaras); se encontraron cuatro sueros con título de 1:64, tres sueros con título 1:128 y un suero presentó titulación de 1:512. Todas las garrapatas (n=933) fueron identificadas taxonómicamente como A. cajennense. Conclusión. En Colombia existen zonas endémicas de rickettsiosis y la aparición de brotes anuales lo confirma (Necoclí, 2006; Los Córdobas, 2007, y Altos de Mulatos, 2008). El presente estudio reporta por primera vez la presencia de infección natural por rickettsia del grupo de las fiebres manchadas en capibaras de Colombia. Los hallazgos sugieren que los capibaras pueden ser usados como potenciales centinelas de la circulación de rickettsias y marcadores de las áreas de riesgo para la transmisión de rickettsiosis. Abstract in english Introduction. Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rodents are usually parasitized by the tick vector, Amblyomma cajennense, the main vector of rickettsioses in humans and animals in South America. Capybaras can be used as sentinels in detecti [...] on of circulation of rickettsiae. Objective. Antibodies to rickettsiae of spotted fever group were detected in capybaras in a rural area of Cordoba Province, northern Colombia. Materials and methods. Sera were analyzed from 36 capybaras in a rural area of Monteria (village of San Jeronimo) in Córdoba. For the detection of IgG antibodies, indirect immunofluorescence was performed. The antigens were derived from R. rickettsia strain Taiaçu isolated in Brazil. Capybara sera were diluted 1:64 for IFA analysis. Ticks were collected from each capybara (also known as chigüiro) and identified to species. Results. The seroprevalence of spotted fever group Rickettsia was 22% (8 capybaras). Four sera had a titer of 1:64, 3 had a titer of 1:128 and one serum had a titer of 1:512. All ticks removed from the capybaras (n=933) were taxonomically identified as Amblyomma cajennense. Conclusion. Colombia has areas endemic for rickettsioses, as indicated by confirmed annual outbreaks. The current study reports the first evidence of natural rickettsial infection of the spotted fever group in capybaras from Colombia. The findings suggest that capybaras can be used as sentinels for the circulation of rickettsiae and can identify endemic areas for the transmission of rickettsial diseases.

Jorge, Miranda; Verónica, Contreras; Yésica, Negrete; Marcelo B, Labruna; Salim, Máttar.

2011-06-01

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Pancreatoduodenectomía durante el embarazo por adenocarcinoma de ampolla de Vater y posterior resección de recurrencia ganglionar con buen resultado a corto y largo plazo / Pancreatoduodenectomy for ampullary adenocarcinoma and and re-intervention for ganglinonar recurrency  

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Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Mujer de 30 años con 13 semanas de su segunda gestación que se presentó con síntomas de anemia severa, hemorragia digestiva alta, dolor epigástrico y baja de peso. Mediante endoscopía alta se diagnosticó un adenocarcinoma bien diferenciado de ampolla de Vater. Se le practicó pancreato-duodenectomía [...] a las 16 semanas de su embarazo sin complicaciones. Durante el seguimiento se identificó recurrencia ganglionar loco-regional 4 meses después por lo que se le realizó parto por cesárea a las 34 semanas con neonato saludable de 2500 gr. Se decidió reintervención para resección de enfermedad ganglionar la cual se realizó con éxito. Lleva 36 meses de seguimiento sin evidencia de recidiva de la enfermedad. Su hija ha tenido un desarrollo normal. Abstract in english A 30 years old woman in the 13 week of her second pregnancy who had severe anemia, upper gastrointestinal bleeding and weight loss. She was given the endoscopic diagnosis of a well differentiated ampullary adenocarcinoma. She underwent a pancreato duodenectomy during the 16 week of pregnancy without [...] complications. After 4 months of follow up we identified a ganglionar local recurrence so that´s why she underwent a cesarean in the 34 week of pregnancy. The product was a healthy 2500 gr. newborn. We decided a reoperation for the resection of the recurrence and it was carried out successfully. Currently the patient has 36 months of follow up without evidence of recurrence and her baby has a normal grow up.

Gustavo, Reaño Paredes; José, De Vinatea De Cárdenas; Fernando, Revoredo Rego; Fritz, Kometter Barrios; Luis, Villanueva Alegre; José, Arenas Gamio; Mónica, Uribe León.

2014-07-01

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Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná  

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Full Text Available INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG. Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene. RESULTS: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6% were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. CONCLUSIONS: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans.INTRODUÇÃO: A febre maculosa é uma zoonose emergente causada por espécies de Rickettsia do grupo febre maculosa (GFM. Rickettsia rickettsii é o principal agente etiológico da febre maculosa brasileira (FMB e é transmitida por Amblyomma spp. MÉTODOS: Com o objetivo de obter informações sobre GFM Rickettsiae no Parque Municipal Arthur Thomas em Londrina, PR, carrapatos de vida livre e de capivaras foram coletados, assim como amostras de sangue das pessoas que trabalham no parque. A. dubitatum e A. cajennense foram submetidos à PCR em pools para analises de Rickettsia spp. gltA (citrate synthase gene. RESULTADOS: Todos os pools de carrapatos analizados foram negativos. Soros de humanos foram testados pela imunofluorescência indireta com antigenos de R. rickettsii e R. parkeri. Entre os 34 soros analisados, 7 (20,6% foram positivos para R. rickettsii. Destes, quatro apresentaram títulos iguais a 64, dois iguais a 128 e um, igual a 256, mas nenhum soro reagiu com R. parkeri. Não houve nenhuma associação, estatisticamente significante, entre as variáveis analisadas no questionário epidemiológico fornecido às pessoas que participaram da pesquisa. CONCLUSÕES: Os estudos sorológicos sugerem a presença de alguma Rickettsiae relacionada ao GFM que poderiam estar infectando a população humana estudada. Entretanto, as análises dos carrapatos foram inconclusivas para determinar qual espécie poderia estar envolvida na transmissão para os humanos.

Roberta Santos Toledo

2011-06-01

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Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia / Ecoepidemiology of rickettsial infection in rodents, ectoparasites and humans in northeastern Antioquia, Colombia  

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Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Introducción. Las rickettsias son bacterias patógenas usualmente transmitidas por ectoparásitos, como garrapatas, piojos o pulgas. En la última década se presentaron tres brotes de rickettsiosis con casos fatales en la región noroccidental de Antioquia y en un municipio limítrofe de Córdoba. Objetiv [...] o. Describir la ecología y la epidemiología de las infecciones por Rickettsia spp. en el Urabá antioqueño. Materiales y métodos. Se obtuvieron muestras de 354 roedores y se recolectaron 839 ectoparásitos de estos en los municipios de Apartadó, Turbo y Necoclí. Asimismo, se obtuvieron 220 sueros humanos. Estas muestras fueron estudiadas por reacción en cadena de la polimerasa (PCR) e inmunofluorescencia indirecta (IFI) para la detección de infección por rickettsias. Resultados. Por IFI se detectaron anticuerpos antirickettsias en 130 (43 %) de los roedores y en 53 (24 %) de los sueros humanos estudiados. Además, se amplificaron secuencias del gen gltA específicas del género Rickettsia en 23 (6,8 %) muestras de hígado de roedores, las cuales mostraron una similitud del 98,7 % con R. prowazekii . Una secuencia de gltA obtenida de larvas de garrapatas del género Amblyomma sp., tuvo una identidad mayor de 99 % con las secuencias de R. tamurae . Conclusión. Estos resultados demuestran la circulación de rickettsias en roedores, ectoparásitos y humanos en los municipios estudiados. Abstract in english Introduction: Rickettsia spp. are tick, flea or lice-borne pathogenic bacterium, usually carried by rodents. In the last decade three outbreaks of rickettsial disease including fatalities, occurred in the provinces of Antioquia and Córdoba in northwestern Colombia. Objective: The purpose of this stu [...] dy was to perform an ecological and epidemiological description of the Rickettsia spp infection in the recently affected region of Colombia. Materials and methods: Samples were obtained from 354 rodents and their parasites captured in the municipalities of Apartadó, Turbo and Necoclí. Likewise, 220 human sera were also collected, for detection of infection by Rickettsia spp. Results: Indirect immunofluorescence assay (IFA) revealed that 130 (43%) of the rodents and 53 (24%) of the humans produced antibodies to Rickettsia spp. Additionally, rickettsial DNA was amplified by PCR from 23 (6.8%) rodent liver samples using primers directed to the genus specific gltA gene. While gltA sequences from rodent samples exhibited a 98.7% similitude with R . prowazekii, a sequence amplified from larvae of Amblyomma sp exhibited identities of >99% similarity with R. tamurae . Conclusion: These results demonstrate the presence of rickettsia in rodents, ectoparasites and humans throughout the municipalities studied.

Juan Carlos, Quintero; Andrés Felipe, Londoño; Francisco J, Díaz; Piedad, Agudelo-Flórez; Margarita, Arboleda; Juan David, Rodas.

2013-09-01

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Surveillance of Rickettsia sp. infection in capybaras (Hydrochoerus hydrochaeris) a potential model of epidemiological alert in endemic areas Vigilancia de la infección por Rickettsia sp. en capibaras (Hydrochoerus hydrochaeris) un modelo potencial de alerta epidemiológica en zonas endémicas  

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Introduction. Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rodents are usually parasitized by the tick vector, Amblyomma cajennense, the main vector of rickettsioses in humans and animals in South America. Capybaras can be used as sentinels in detection of circulation of rickettsiae.
Objective. Antibodies to rickettsiae of spotted fever group were detected in capybaras in a rural area of Cordoba Province, northern Colombia.

Labruna, Marcelo B.; Salim Mattar; Jorge Miranda; Yesica Negrete; Verónica Contreras

2011-01-01

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Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil / Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese INTRODUÇÃO: A febre maculosa é uma zoonose emergente causada por espécies de Rickettsia do grupo febre maculosa (GFM). Rickettsia rickettsii é o principal agente etiológico da febre maculosa brasileira (FMB) e é transmitida por Amblyomma spp. MÉTODOS: Com o objetivo de obter informações sobre GFM Ri [...] ckettsiae no Parque Municipal Arthur Thomas em Londrina, PR, carrapatos de vida livre e de capivaras foram coletados, assim como amostras de sangue das pessoas que trabalham no parque. A. dubitatum e A. cajennense foram submetidos à PCR em pools para analises de Rickettsia spp. gltA (citrate synthase gene). RESULTADOS: Todos os pools de carrapatos analizados foram negativos. Soros de humanos foram testados pela imunofluorescência indireta com antigenos de R. rickettsii e R. parkeri. Entre os 34 soros analisados, 7 (20,6%) foram positivos para R. rickettsii. Destes, quatro apresentaram títulos iguais a 64, dois iguais a 128 e um, igual a 256, mas nenhum soro reagiu com R. parkeri. Não houve nenhuma associação, estatisticamente significante, entre as variáveis analisadas no questionário epidemiológico fornecido às pessoas que participaram da pesquisa. CONCLUSÕES: Os estudos sorológicos sugerem a presença de alguma Rickettsiae relacionada ao GFM que poderiam estar infectando a população humana estudada. Entretanto, as análises dos carrapatos foram inconclusivas para determinar qual espécie poderia estar envolvida na transmissão para os humanos. Abstract in english INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG). Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF) and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae [...] in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene). RESULTS: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6%) were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. CONCLUSIONS: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans.

Roberta Santos, Toledo; Katia, Tamekuni; Mauro de Freitas, Silva Filho; Valeska Bender, Haydu; Richard Campos, Pacheco; Marcelo Bahia, Labruna; John Stephen, Dumler; Odilon, Vidotto.

2011-06-01

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Tuberculose ganglionar: desafio diagnóstico / Tuberculous lymphadenitis: diagnostic challenge  

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Full Text Available SciELO Portugal | Language: Portuguese Abstract in portuguese A tuberculose ganglionar é a forma extrapulmonar mais comum de infecção por Mycobacterium tuberculosis. Não obstante, o mimetismo que mantém com outras patologias leva, comumente, a falha ou atraso na obtenção do diagnóstico correto. Os autores apresentam o caso clínico de tuberculose ganglionar num [...] a doente de 89 anos de idade, com múltiplas co-morbilidades, internada para esclarecimento de quadro constitucional caracterizado por febre e adenopatias cervicais de evolução indolente. Abstract in english Tuberculous lymphadenitis is the most common form of extrapulmonary Mycobacterium tuberculosis infection. Nevertheless, the mimicry that keeps with other pathologies leads, commonly, to failure or delay in obtaining a correct diagnosis. The authors present the case of tuberculous lymphadenitis in a [...] 89 years old female, with multiple co-morbidities, admitted with constitutional framework characterized by fever and cervical lymphadenopathy.

Miguel, Neno; Cláudia, Rocha; Dora, Sargento; Glória, Silva.

2014-02-01

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First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro / Primeira identificação de infecção natural por Rickettsia rickettsii no carrapato Rhipicephalus sanguineus no Rio de Janeiro  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese A Febre Maculosa Brasileira (FMB) é uma zoonose causada por Rickettsia rickettsii e transmitida por carrapatos do gênero Amblyomma, mais freqüentemente pela espécie Amblyomma cajennense. Este trabalho tem como objetivo relatar a primeira detecção molecular de R. rickettsii em Rhipicephalus sanguineu [...] s naturalmente infectado no Rio de Janeiro, Brasil. Carrapatos foram coletados de cães, procedentes de uma região rural do município de Resende, estado do Rio de Janeiro, Brasil (22º30'9.46"S, 44º42'44.29"WO), onde ocorreram cinco casos humanos de FMB em 2006. Todos os carrapatos foram identificados segundo chave dicotômica, utilizando-se lupa estereoscópica e separados de acordo com estágio, espécie e sexo. Para a extração de DNA utilizou-se o kit comercial QIAamp DNA (QIAGEN ®). O DNA foi submetido à técnica de PCR utilizando 04 conjuntos de iniciadores para a amplificação dos genes: Rr190.70p/Rr190.602n (OmpA, 532bp), BG1-21/BG2-20 (OmpB, 650bp), Tz15/Tz16 (17 kDa gene que codifica a proteína, 246bp) e RPCs .877p/RpCS.1258n (gltA, 381bp). Os produtos da PCR foram separados por eletroforese em gel agarose 1% corados com brometo de etídio e visualizados sob luz ultravioleta e, aqueles que apresentaram bandas amplificadas foram purificados utilizando-se o kit comercial QIAquick ® e seqüenciados pelo ABI PRISM®. As seqüências nucleotídicas foram geradas usando Bioedit®, editado em software e comparados os correspondentes homólogos com as sequências disponíveis através GenBank, utilizando Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov). Confirmou-se R. rickettsii (GenBank FJ356230) no seqüenciamento de apenas um espécime, adulto de carrapato R. sanguineus. A caracterização molecular de R. rickettsii em exemplar de carrapato R. sanguineus confirma que esta espécie pode ter importante papel na transmissão de R. rickettsii para humanos no território brasileiro. Abstract in english The Brazilian Spotted Fever (BSF) is a zoonotic disease caused by Rickettsia rickettsii and transmitted by ticks of the genus Amblyomma, more frequently, Amblyomma cajennense. The aim of this paper was to report the first molecular detection of R. rickettsii on R. sanguineus naturally infected in Ri [...] o de Janeiro, Brazil. Ticks were collected from dogs in a rural region of Resende municipality, Rio de Janeiro State, Brazil (22º30'9.46"S, 44º42'44.29"WO), where occurred five human cases of BSF in 2006. The ticks were identified under a stereoscopic microscope and separated in pools by stages, species and sex. DNA extraction was carried out using QIAamp DNA Mini Kit (QIAGEN®). The DNA was submitted to PCR amplification using 04 set of primers: Rr190.70p/Rr190.602n (OmpA, 532bp), BG1-21/BG2-20 (OmpB, 650bp), Tz15/Tz16 (17 kDa protein-encoding gene, 246bp) and RpCS.877p/RpCS.1258n (gltA, 381bp). PCR products were separated by electrophoresis on 1% agarose gels and visualized under ultraviolet light with ethidium bromide. PCR products of the expected sizes were purified by QIAquick® and sequenced by ABI PRISM®. The generated nucleotide sequences were edited with using Bioedit® software and compared with the corresponding homologous sequences available through GenBank, using Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov). It was confirmed R. rickettsii by sequencing of the material (GenBank FJ356230). The molecular characterization of R. rickettsii in the tick R. sanguineus emphasizes the role of dogs as carriers of ticks from the environment to home. Moreover, this result suggests that there is a considerable chance for active participation of R. sanguineus as one of tick species in the transmission of R. ricketsii to human being in the Brazilian territory.

Nathalie C., Cunha; Adivaldo H., Fonseca; Jania, Rezende; Tatiana, Rozental; Alexsandra R.M., Favacho; Jairo D., Barreira; Carlos L., Massard; Elba R.S., Lemos.

2009-02-01

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First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro Primeira identificação de infecção natural por Rickettsia rickettsii no carrapato Rhipicephalus sanguineus no Rio de Janeiro  

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Full Text Available The Brazilian Spotted Fever (BSF is a zoonotic disease caused by Rickettsia rickettsii and transmitted by ticks of the genus Amblyomma, more frequently, Amblyomma cajennense. The aim of this paper was to report the first molecular detection of R. rickettsii on R. sanguineus naturally infected in Rio de Janeiro, Brazil. Ticks were collected from dogs in a rural region of Resende municipality, Rio de Janeiro State, Brazil (22º30'9.46"S, 44º42'44.29"WO, where occurred five human cases of BSF in 2006. The ticks were identified under a stereoscopic microscope and separated in pools by stages, species and sex. DNA extraction was carried out using QIAamp DNA Mini Kit (QIAGEN®. The DNA was submitted to PCR amplification using 04 set of primers: Rr190.70p/Rr190.602n (OmpA, 532bp, BG1-21/BG2-20 (OmpB, 650bp, Tz15/Tz16 (17 kDa protein-encoding gene, 246bp and RpCS.877p/RpCS.1258n (gltA, 381bp. PCR products were separated by electrophoresis on 1% agarose gels and visualized under ultraviolet light with ethidium bromide. PCR products of the expected sizes were purified by QIAquick® and sequenced by ABI PRISM®. The generated nucleotide sequences were edited with using Bioedit® software and compared with the corresponding homologous sequences available through GenBank, using Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov. It was confirmed R. rickettsii by sequencing of the material (GenBank FJ356230. The molecular characterization of R. rickettsii in the tick R. sanguineus emphasizes the role of dogs as carriers of ticks from the environment to home. Moreover, this result suggests that there is a considerable chance for active participation of R. sanguineus as one of tick species in the transmission of R. ricketsii to human being in the Brazilian territory.A Febre Maculosa Brasileira (FMB é uma zoonose causada por Rickettsia rickettsii e transmitida por carrapatos do gênero Amblyomma, mais freqüentemente pela espécie Amblyomma cajennense. Este trabalho tem como objetivo relatar a primeira detecção molecular de R. rickettsii em Rhipicephalus sanguineus naturalmente infectado no Rio de Janeiro, Brasil. Carrapatos foram coletados de cães, procedentes de uma região rural do município de Resende, estado do Rio de Janeiro, Brasil (22º30'9.46"S, 44º42'44.29"WO, onde ocorreram cinco casos humanos de FMB em 2006. Todos os carrapatos foram identificados segundo chave dicotômica, utilizando-se lupa estereoscópica e separados de acordo com estágio, espécie e sexo. Para a extração de DNA utilizou-se o kit comercial QIAamp DNA (QIAGEN ®. O DNA foi submetido à técnica de PCR utilizando 04 conjuntos de iniciadores para a amplificação dos genes: Rr190.70p/Rr190.602n (OmpA, 532bp, BG1-21/BG2-20 (OmpB, 650bp, Tz15/Tz16 (17 kDa gene que codifica a proteína, 246bp e RPCs .877p/RpCS.1258n (gltA, 381bp. Os produtos da PCR foram separados por eletroforese em gel agarose 1% corados com brometo de etídio e visualizados sob luz ultravioleta e, aqueles que apresentaram bandas amplificadas foram purificados utilizando-se o kit comercial QIAquick ® e seqüenciados pelo ABI PRISM®. As seqüências nucleotídicas foram geradas usando Bioedit®, editado em software e comparados os correspondentes homólogos com as sequências disponíveis através GenBank, utilizando Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov. Confirmou-se R. rickettsii (GenBank FJ356230 no seqüenciamento de apenas um espécime, adulto de carrapato R. sanguineus. A caracterização molecular de R. rickettsii em exemplar de carrapato R. sanguineus confirma que esta espécie pode ter importante papel na transmissão de R. rickettsii para humanos no território brasileiro.

Nathalie C. Cunha

2009-02-01

13

AFECTACIÓN GANGLIONAR EN PACIENTE CON ENDOMETRIOSIS PROFUNDA RECTOVAGINAL  

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Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Se describe un raro caso de endometriosis rectovaginal con compromiso ganglionar en mujer de 33 años. La presencia de tejido endometrial en ganglios linfáticos pélvicos es rara y ha sido confirmada en la literatura en mujeres que han sido sometidas a cirugía por endometriosis. La presencia de endome [...] triosis en los ganglios linfáticos pélvicos es muy improbable que surja de novo y sugiere extensión de la enfermedad. Abstract in english A rare case of rectovaginal endometriosis with lymph node involvement is described in a 33-year-old patient. The presence of endometrial tissue in pelvic lymph nodes is rare and has been confirmed in the literature in subjects who underwent surgery for endometriosis. Involvement of pelvic lymph node [...] s by endometriosis seems unlikely to arise de novo and probably suggests lymphatic spread of the disease.

Israel, Ortega Sánchez; Bárbara, Castro Martín; Javier, de Santiago García; Alicia, Hernández Gutiérrez; Dulce Mª, Benito López; Juan, Ordás Santo Tomás.

14

Rickettsia-Macrophage Interactions: Host Cell Responses to Rickettsia akari and Rickettsia typhi  

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The existence of intracellular rickettsiae requires entry, survival, and replication in the eukaryotic host cells and exit to initiate new infection. While endothelial cells are the preferred target cells for most pathogenic rickettsiae, infection of monocytes/macrophages may also contribute to the establishment of rickettsial infection and resulting pathogenesis. We initiated studies to characterize macrophage-Rickettsia akari and -Rickettsia typhi interactions and to determine how rickettsi...

Radulovic, S.; Price, P. W.; Beier, M. S.; Gaywee, J.; Macaluso, J. A.; Azad, A.

2002-01-01

15

Environmental infestation and rickettsial infection in ticks in an area endemic for Brazilian spotted fever / Infestacao ambiental e infeccao por rickettsias em carrapatos de area endemica para Febre Maculosa Brasileira  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese A Febre Maculosa Brasileira (FMB) é uma antropozoonose endêmica no município de Americana/SP, causada pela bactéria Rickettsia rickettsii e transmitida pelo carrapato Amblyomma cajennense. Este estudo avaliou a fauna de carrapatos e a infecção por riquétsias em carrapatos de vida livre capturados me [...] nsalmente com armadilhas de CO2, em áreas de risco para FMB de Americana, de julho de 2009 a junho de 2010. Duas espécies foram capturadas, A. cajennense (6.122 larvas; 4.265 ninfas; 2.355 adultos) e Amblyomma dubitatum (7.814 larvas; 3.364 ninfas; 1.193 adultos). Os estágios imaturos de A. cajennense e A. dubitatum apresentaram uma distribuição anual semelhante, com larvas de ambas as espécies sendo coletadas em maior número no período de abril a julho e ninfas de junho a outubro. Maior número de adultos de A. cajennense foi coletado de outubro a dezembro, enquanto que os adultos de A. dubitatum foram coletados em número relativamente semelhante durante todo o ano. A infecção por Rickettsia foi avaliada pela PCR em 1157 carrapatos A. cajennense e 1040 A. dubitatum, com apenas 41 (3,9%) A. dubitatum infectados com Rickettsia bellii. Este estudo demonstrou que as áreas de risco para FMB de Americana são caracterizadas por elevadas infestações ambientais de A. cajennense e A. dubitatum. Abstract in english Brazilian spotted fever (BSF), caused by Rickettsia rickettsii, is endemic in the municipality of Americana, southeastern Brazil, where the disease is transmitted by the tick Amblyomma cajennense. This study evaluated the tick fauna and rickettsial infection in free-living ticks that were captured m [...] onthly using dry ice traps in areas endemic for BSF in Americana, from July 2009 to June 2010. Two tick species were captured: A. cajennense (6,122 larvae; 4,265 nymphs; 2,355 adults) and Amblyomma dubitatum (7,814 larvae; 3,364 nymphs; 1,193 adults). The immature stages of A. cajennense and A. dubitatum had similar distribution through the 12-month period, with larvae of both species collected in highest numbers between April and July, and nymphs between June and October. The highest numbers of A. cajennense adults were collected between October and December, whereas A. dubitatum adults were collected in relatively similar numbers throughout the 12-month period. Rickettsial infection was evaluated by means of PCR in 1,157 A. cajennense and 1,040 A. dubitatum ticks; only 41 (3.9%) A. dubitatum were found to be infected by Rickettsia bellii. The present study showed that the areas of Americana that are endemic for BSF are characterized by high environmental burdens of A. cajennense and A. dubitatum.

Jose, Brites-Neto; Fernanda Aparecida, Nieri-Bastos; Jardel, Brasil; Keila Maria Roncato, Duarte; Thiago Fernandes, Martins; Cecilia Jose, Verissimo; Amalia Regina Mar, Barbieri; Marcelo Bahia, Labruna.

2013-09-01

16

Febre maculosa: isolamento de Rickettsia em amostra de biópsia de pele / Spotted fever: Rickettsia isolation in skin biopsy sample  

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Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Presença de Rickettsia na pele de doente de Febre Maculosa foi evidenciada por inoculação intraperitoneal em cobaio. O diagnóstico sorológico por imunoflüorescência indireta revelou diferença de título de anticorpos específicos para Rickettsia rickettsii, de 4 vezes entre a 1º e a 3º amostra. Imunog [...] lobulina M (IgM) específica foi detectada nas amostras de sangue, evidência de infecção em atividade ou recente. Foi também detectada a presença de anticorpos específicos para R. rickettsii no soro dos cobaios inoculados. Abstract in english A 2 years old child living in an area of the State of São Paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. Rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. In sera tested by indirect immunofluorescence [...] with Rickettsia rickettsii standard antigen, IgG specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; IgM specific antibody titer was 1:128 in the three samples. Also positive were sera obtained from the inoculated guinea pigs. In the last 20 years no other case of rickettsial spotted fever has been confirmed by isolation of the agent in Brasil. To our knowlwdge, there are no previous reports of isolation of Rickettsiae through inoculation of skin biopsy homogenates.

Heloisa Helana B., Melles; Silvia, Colombo; Marcos Vinícius da, Silva.

1992-02-01

17

Factores predictores de metástasis ganglionar en el carcinoma diferenciado de tiroides  

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Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish Antecedentes: son necesarios factores pronósticos confiables de metástasis ganglionar para adaptar el tratamiento quirúrgico inicial de pacientes con carcinoma diferenciado de tiroides. Objetivo: determinar la frecuencia y factores pronósticos asociados con metástasis ganglionar en pacientes operado [...] s por carcinoma diferenciado de tiroides. Lugar de apicación: práctica privada. Diseño: retrospectivo observacional. Pobación: entre enero de 2000 y agosto de 2010, a 600 pacientes con 639 tumores (39 bilaterales) se les realizó tiroidectomía total y linfadenectomía terapéutica sólo cuando se demostró metástasis por biopsia ganglionar. Método: revisión de historias clínicas e informes patológicos. Resutados: 145 enfermos (22.7 %) tuvieron ganglios histológicamente positivos. El análisis multivariado mostró que la edad menor de 45 años (p = 0.001), adenopatías palpables (p = 0.0001), multicentricidad (p = 0.005) e invasión extracapsular (p = 0.0001) fueron factores de riesgo independientes de metástasis ganglionar. Estos factores, en conjunto, tuvieron una alta especificidad (97 %)y una baja sensibilidad (40 %). Se encontraron metástasis en ganglios yugulares con ganglios centrales negativos ("skip" metástasis) en 29 casos (5.54 %). Concusiones: a pesar de que algunos de los factores estudiados tuvieron valor pronóstico, se requieren variables adicionales para definir mejor el manejo quirúrgico. Abstract in english Background: reliable prognostic factors of lymph node metástasis are needed to adapt initial surgical treatment of patients with differentiated thyroid carcinoma. Objetive: to determine the frequency and predictive factors associated with lymph node metástasis in patients operated on for differentia [...] ted thyroid carcinoma. Setting: prívate practice. Design: retrospective observational. Popuation: between January 2000 and August 2010, 600 patients with 639 tumours (39 bilateral) underwent total thyroidectomy and therapeutic neck dissection only when there was biopsy proved lymph node metástasis. Method: review of clinical records and pathological reports. Resuts: 145 patients (22.7 %) had histologically positive lymph nodes. Multivariate analysis showed that lessthan 45 years (p = 0.001), palpable adenopathy (p = 0.0001), multicentricity (p = 0.005) and extracapsular invasión (p = 0.0001) were independent risk factors of lymph node metástasis. These factors, together, had high specificity (97 %) but low sensibility (40 %). Metástasis in jugular lymph nodes with normal central nodes (skip metástasis) was found in 29 (5.54 %) cases. Concusions: even though some of the factors studied proved to be of prognostic valué, additional variables are needed to better define surgical management.

Jorge E, Falco; Alvaro, Otero Muñoz; Manuel R, Montesinos.

2012-12-01

18

Seroprevalencia de la infección por Borrelia burgdorgferi y Rickettsia conorii en población humana y canina de la zona básica de salud de San Andrés del Rabanedo (León, España) / Seroprevalence of the infection caused by Borrelia burgdorferi and Rickettsia conorii in human and canine population in the basic health area of San Andrés del Rabanedo (León, Spain)  

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Full Text Available SciELO Public Health | Language: Spanish Abstract in spanish FUNDAMENTOS: Se estudia la seroprevalencia de la infección por Borrelia burgdorferi y Rickettsia conorii en población humana y canina para conocer la situación de ambas en humanos, al mismo tiempo que la significación del perro, como indicador de la circulación de estos agentes entre aquéllos, en la [...] Zona de Salud de San Andrés del Rabanedo, León. MÉTODO: Se realizó un estudio en 98 sueros humanos y 95 caninos (de diversas razas y aptitudes) frente a B. burgdorferi (títulos de positividad > a 1/128 y > a 1/64 o superiores, respectivamente) y 104 sueros humanos y 84 caninos frente a R. conorii (positividad a título > a 1/64 o superiores en ambas especies) mediante inmunofluorescencia indirecta (IFI). RESULTADOS: Se halló positividad a las dos infecciones, tanto en personas como en perros. Frente a B. burgdorferi fue superior en humanos que en perros y frente a R. conorii fue superior en éstos que en humanos. En personas fue del 4,08% frente a B. burgdorferi y 1% frente a R. conorii; en perros fue del 2,10% frente a B. burgdorferi y del 14,28% frente a R. conorii. Los valores más altos se hallaron en los meses de primavera-verano, salvo en el caso de B. burgdorferi en perros. La seroprevalencia fue mayor en perros dedicados al cuidado de ganado (ovino) que en los de caza y guarda. CONCLUSIONES: Los porcentajes de seroprevalencia hallados en nuestro trabajo, tanto en seres humanos como en caninos, considerados en el ámbito territorial de una zona geográfica semi-rural de la provincia de León, han sido iguales o inferiores a los reseñados para otras provincias, incluida la totalidad de la de León. En perros se halló mayor seroprevalencia frente a R. conorii que frente a B. burgdorferi, lo que indica que es el agente más extendido en nuestra Provincia, como han señalado otros autores. Los valores hallados en seres humanos frente a B. burgdorferi han sido más altos que en perros; la existencia de reacciones cruzadas con otros microorganismos ha podido influenciar estos resultados. Por ello, consideramos necesario realizar más estudios de prevalencia de estas infecciones para una vigilancia epidemiológica adecuada y control de estas zoonosis, dada su repercusión en salud pública. Abstract in english BACKGROUND: Positive results in infections of borrelia burgdorferi and Rickettsia conorii in human and canine population is studied in order to uderstand the situation of both in humans, and at the same time discover the importance of the dog as an indicator of these agents amongst those in the Heal [...] th Area of San Andrés del Rabanedo, León. METHODS: A study was made of 98 human serums and 95 canine serums (dogs of different breeds and capabilities) as regards B. burgdorferi (positive results > at 1/128 and > at 1/64 or above, respectively) and 104 human serums and 84 canine serums as regards R. conorii (postive results at range > at 1/64 or above in both species) by means of indirect Immunofluorescence (IFI). RESULTS: Positivity in both infections was discovered in both humans and dogs. With regard to B. burgdorferi it was higher in humans than in dogs and with regard to R. conorii it was higher in dogs than in humans. In humans it was 4,08% as regards B. burgdorferi and 1% with respect to R. conorii; in dogs it was 2,10% as regards B. burgdorferi and 14,28% regarding R. conorii. The highest values were discovered in the Spring-Summer months except in the case of B. burgdorferi in dogs. Serum prevalence was greater in dogs used to guard other animals (sheep) than those involved in hunting and security. CONCLUSIONS: The percentages of positive results discovered in our work, in humans as well as dogs, estimated in the territorial area of a geographical zone in a semi-rural León province, were equal or inferior to those discovered in other provinces, including ours. In dogs there were larger positive results regarding R. conorii than B. burgdorferi, which indicates that it is the most extended agent within our Pro

Jaime, Rojo Vázquez.

1997-03-01

19

Seroprevalencia de la infección por Borrelia burgdorgferi y Rickettsia conorii en población humana y canina de la zona básica de salud de San Andrés del Rabanedo (León, España) / Seroprevalence of the infection caused by Borrelia burgdorferi and Rickettsia conorii in human and canine population in the basic health area of San Andrés del Rabanedo (León, Spain)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish FUNDAMENTOS: Se estudia la seroprevalencia de la infección por Borrelia burgdorferi y Rickettsia conorii en población humana y canina para conocer la situación de ambas en humanos, al mismo tiempo que la significación del perro, como indicador de la circulación de estos agentes entre aquéllos, en la [...] Zona de Salud de San Andrés del Rabanedo, León. MÉTODO: Se realizó un estudio en 98 sueros humanos y 95 caninos (de diversas razas y aptitudes) frente a B. burgdorferi (títulos de positividad > a 1/128 y > a 1/64 o superiores, respectivamente) y 104 sueros humanos y 84 caninos frente a R. conorii (positividad a título > a 1/64 o superiores en ambas especies) mediante inmunofluorescencia indirecta (IFI). RESULTADOS: Se halló positividad a las dos infecciones, tanto en personas como en perros. Frente a B. burgdorferi fue superior en humanos que en perros y frente a R. conorii fue superior en éstos que en humanos. En personas fue del 4,08% frente a B. burgdorferi y 1% frente a R. conorii; en perros fue del 2,10% frente a B. burgdorferi y del 14,28% frente a R. conorii. Los valores más altos se hallaron en los meses de primavera-verano, salvo en el caso de B. burgdorferi en perros. La seroprevalencia fue mayor en perros dedicados al cuidado de ganado (ovino) que en los de caza y guarda. CONCLUSIONES: Los porcentajes de seroprevalencia hallados en nuestro trabajo, tanto en seres humanos como en caninos, considerados en el ámbito territorial de una zona geográfica semi-rural de la provincia de León, han sido iguales o inferiores a los reseñados para otras provincias, incluida la totalidad de la de León. En perros se halló mayor seroprevalencia frente a R. conorii que frente a B. burgdorferi, lo que indica que es el agente más extendido en nuestra Provincia, como han señalado otros autores. Los valores hallados en seres humanos frente a B. burgdorferi han sido más altos que en perros; la existencia de reacciones cruzadas con otros microorganismos ha podido influenciar estos resultados. Por ello, consideramos necesario realizar más estudios de prevalencia de estas infecciones para una vigilancia epidemiológica adecuada y control de estas zoonosis, dada su repercusión en salud pública. Abstract in english BACKGROUND: Positive results in infections of borrelia burgdorferi and Rickettsia conorii in human and canine population is studied in order to uderstand the situation of both in humans, and at the same time discover the importance of the dog as an indicator of these agents amongst those in the Heal [...] th Area of San Andrés del Rabanedo, León. METHODS: A study was made of 98 human serums and 95 canine serums (dogs of different breeds and capabilities) as regards B. burgdorferi (positive results > at 1/128 and > at 1/64 or above, respectively) and 104 human serums and 84 canine serums as regards R. conorii (postive results at range > at 1/64 or above in both species) by means of indirect Immunofluorescence (IFI). RESULTS: Positivity in both infections was discovered in both humans and dogs. With regard to B. burgdorferi it was higher in humans than in dogs and with regard to R. conorii it was higher in dogs than in humans. In humans it was 4,08% as regards B. burgdorferi and 1% with respect to R. conorii; in dogs it was 2,10% as regards B. burgdorferi and 14,28% regarding R. conorii. The highest values were discovered in the Spring-Summer months except in the case of B. burgdorferi in dogs. Serum prevalence was greater in dogs used to guard other animals (sheep) than those involved in hunting and security. CONCLUSIONS: The percentages of positive results discovered in our work, in humans as well as dogs, estimated in the territorial area of a geographical zone in a semi-rural León province, were equal or inferior to those discovered in other provinces, including ours. In dogs there were larger positive results regarding R. conorii than B. burgdorferi, which indicates that it is the most extended agent within our Province, a

Jaime, Rojo Vázquez.

1997-03-01

20

Rickettsia felis in Fleas, Germany  

OpenAIRE

Among 310 fleas collected from dogs and cats in Germany, Rickettsia felis was detected in all specimens (34) of Archaeopsylla erinacei (hedgehog flea) and in 9% (24/226) of Ctenocephalides felis felis (cat flea). R. helvetica was detected in 1 Ceratophyllus gallinae (hen flea).

Gilles, Je?re?mie; Just, Frank Thomas; Silaghi, Cornelia; Pradel, Ingrid; Passos, Lygia Maria Friche; Lengauer, Heidi; Hellmann, Klaus; Pfister, Kurt

2008-01-01

21

Permeability of Rickettsia prowazekii to NAD.  

OpenAIRE

Rickettsia prowazekii accumulated radioactivity from [adenine-2,8-3H]NAD but not from [nicotinamide-4-3H]NAD, which demonstrated that NAD was not taken up intact. Extracellular NAD was hydrolyzed by rickettsiae with the products of hydrolysis, nicotinamide mononucleotide and AMP, appearing in the incubation medium in a time- and temperature-dependent manner. The particulate (membrane) fraction contained 90% of this NAD pyrophosphatase activity. Rickettsiae which had accumulated radiolabel aft...

Atkinson, W. H.; Winkler, H. H.

1989-01-01

22

Ganglio postcentinela en melanoma: nuevo método diagnóstico para la detección de metástasis ganglionares pélvicas Postsentinel lymph node in melanoma: a new diagnostic method for detection of metastatic pelvic lymph nodes  

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Full Text Available Los ganglios inguinales son el primer sitio de afectación metastásica del melanoma primario de las extremidades inferiores. Cuando se comprueba metástasis ganglionares inguinales no es claro si solamente se debe realizar linfadenectomía inguinal superficial (linfadenectomía ganglionar limitada o si por el contrario, se debe efectuar linfadenectomía pélvica (cadena ganglionar ilíaca externa y obturador, además de la linfadenectomía inguinal superficial (linfadenectomía ganglionar extendida o combinada. Se propone al ganglio postcentinela como nuevo método diagnóstico capaz de predecir el compromiso ganglionar pélvico y así reducir el número de vaciamientos pélvicos innecesarios.Inguinal nodes are the first site of metastatic location of a primary melanoma of the lower extremities. Once inguinal metastatic lymph nodes are demonstrated, it is still not clear if the best conduct is to perform a superficial inguinal lymphadenectomy (limited lymphadenectomy or, on the contrary, a pelvic lymphadenectomy (extended or combined inguinal lymphadenectomy. It is proposed the postsentinel node as anew diagnostic method capable of predicting pelvic node involvement and thus reducing the number of unnecessary pelvic dissections.

Eduardo Torregroza-Diazgranados

2007-03-01

23

Ganglio postcentinela en melanoma: nuevo método diagnóstico para la detección de metástasis ganglionares pélvicas / Postsentinel lymph node in melanoma: a new diagnostic method for detection of metastatic pelvic lymph nodes  

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Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Los ganglios inguinales son el primer sitio de afectación metastásica del melanoma primario de las extremidades inferiores. Cuando se comprueba metástasis ganglionares inguinales no es claro si solamente se debe realizar linfadenectomía inguinal superficial (linfadenectomía ganglionar limitada) o si [...] por el contrario, se debe efectuar linfadenectomía pélvica (cadena ganglionar ilíaca externa y obturador), además de la linfadenectomía inguinal superficial (linfadenectomía ganglionar extendida o combinada). Se propone al ganglio postcentinela como nuevo método diagnóstico capaz de predecir el compromiso ganglionar pélvico y así reducir el número de vaciamientos pélvicos innecesarios. Abstract in english Inguinal nodes are the first site of metastatic location of a primary melanoma of the lower extremities. Once inguinal metastatic lymph nodes are demonstrated, it is still not clear if the best conduct is to perform a superficial inguinal lymphadenectomy (limited lymphadenectomy) or, on the contrary [...] , a pelvic lymphadenectomy (extended or combined inguinal lymphadenectomy). It is proposed the postsentinel node as anew diagnostic method capable of predicting pelvic node involvement and thus reducing the number of unnecessary pelvic dissections.

Eduardo, Torregroza-Diazgranados; Javier Ángel, Aristizábal; Luis Fernando, Viaña; Juan David, Figueroa.

2007-03-01

24

Isolamento de Rickettsia em cultura de células vero / Isolation of Rickettsia in vero cell culture  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Embora o diagnóstico da febre maculosa baseie-se em sinais e sintomas característicos, o mesmo requer confirmação laboratorial, pois existem alguns diagnósticos diferenciais possíveis como meningococcemia, leptospirose, infecção por enterovírus e febre tifóide. A confirmação laboratorial pode ser fe [...] ita através da pesquisa de anticorpos específicos, possível somente alguns dias após o aparecimento da doença, através do isolamento do agente em amostras de sangue e/ou biópsia de pele, e ainda, de amostras de carrapatos coletados do paciente ou de animais reservatório. O isolamento a partir de sangue ou biópsia de pele resulta em diagnóstico precoce da doença, pois na fase de rickettsemia ainda não há anticorpos detectáveis no sangue. Assim, com o objetivo de facilitar o diagnóstico precoce da febre maculosa, estabelecemos um método de isolamento de rickettsia em cultura de células vero. Para a padronização foi inoculada amostra padrão de Rickettsia rickettsii, cepa Sheyla Smith, cedida pelo CDC. A identificação foi feita através da reação de imunofluorescência indireta. A presença de microrganismos verdes fluorescentes visualizados no interior do citoplasma das células caracterizou o crescimento do agente. Posteriormente, a metodologia foi confirmada pelo isolamento do agente da febre maculosa em amostras de biópsia de pele de paciente proveniente de área endêmica no Estado de São Paulo, bem como, de amostras de carrapato do gênero Amblyomma, considerado o reservatório e transmissor da doença no Brasil. Abstract in english The diagnosis of spotted fever is based on characteristic signs and symptoms but requires laboratorial confirmation because of the possible differencial diagnosis from other diseases like leptospirosis, enterovirosis, meningococcemia and tiphoid fever. Laboratorial confirmation may be done by detect [...] ion of specific antibodies which is possible only 5-10 days after the onset of the symptoms or by the isolation of Rickettsiae from blood and/or skin biopsy and from ticks collected in the patient or in the animal reservoir. The isolation of Rickettsiae from blood or skin biopsy results in an early diagnosis of spotted fever since in the rickettsiemic phase of the disease there is no detectable level of antibodies in the serum. With the purpose of facilitating the diagnosis of Spotted Fever we have standardized the isolation of Rickettsiae in cell culture by a method that is less time consuming and that reduces the biological risks than isolation in guinea pigs. vero cell cultures were inoculated with the Sheyla Smith strain of Rickettsia rickettsii provided by CDC (Atlanta-USA). The identification was performed by indirect immunofluorescence technique. The presence of green fluorescent organisms characterized the growth of the agent. Ulterior confirmation of the methodology was done by isolation of the spotted fever agent from skin biopsy of a patient from an endemic area and from Amblyomma ticks that are the reservoir and vector of the Brazilian spotted fever.

Heloisa Helena Barbosa, Melles; Silvia, Colombo; Elba Regina Sampaio de, Lemos.

1999-10-01

25

O estadiamento ganglionar na actualidade / Node staging today  

Scientific Electronic Library Online (English)

Full Text Available SciELO Portugal | Language: Portuguese Abstract in portuguese A necessidade de realizar Esvaziamento Ganglionar Axilar quando o Gânglio Sentinela está metastizado, em doentes com Cancro da Mama, está a ser questionada. A maior parte dos doentes não apresenta metástases adicionais em Gânglios Não Sentinela e, em muito casos, a decisão de realizar tratamentos ad [...] juvantes não necessita da informação adicional fornecida pelo estudo dos gânglios axilares. A publicação recente do ensaio randomizado ACOSOG Z0011veio relançar a discussão sobre a necessidade e a utilidade do Esvaziamento Ganglionar Axilar. Abstract in english The need to perform an Axillary Dissection when the Sentinel Node is metastasized, in Breast Cancer patients, is being questioned. Most part of the patients do not have any additional metastasis in the Non Sentinel Nodes and, in many cases, the decision to perform adjuvant treatments is not dependen [...] t on the axillary nodes analysis. The recent publication of the randomized trial ACOSOG Z0011 launched the discussion on the need and usefulness of the axillary lymph node dissection.

José Luís, Fougo; Fernando, Osório; Susy, Costa; André, Magalhães.

2013-12-01

26

Rickettsia felis: un patógeno emergente en Latinoamérica / Rickettsia felis: an emerging pathogen in Latin America  

Scientific Electronic Library Online (English)

Full Text Available SciELO Costa Rica | Language: Spanish Abstract in spanish El primer caso humano infectado con Rickettsia felis fue descrito en un paciente diagnosticado con tifus murino en Estados Unidos en 1994. Los reportes acerca de la presencia de R. felis se han incrementado en los últimos años y ya se ha reportado en la mayoría de los países de América Latina. Los s [...] íntomas y signos de la enfermedad causada por R. felis son inespecíficos y es importante que cada país lo incluya en el diagnóstico diferencial con respecto a otras enfermedades que tienen las mismas manifestaciones clínicas. Abstract in english The first human case of Rickettsia felis was described in 1994, in a patient diagnosed with murine typhus in the United States. Reports about the presence of R. felis have increased in the last years, and it has been reported in most countries of Latin America. Signs and symptoms of disease caused b [...] y R. felis are unspecific, and it is important for each country to include it in the differential diagnosis with other diseases that show the same clinical manifestations.

Jorge E, Zavala Castro.

2013-07-01

27

Rickettsia as obligate and mycetomic bacteria.  

Science.gov (United States)

Rickettsiae are well known as intracellular pathogens of animals, humans, and plants and facultative and unorganized symbionts of invertebrates. No close relative of mitochondria has yet been associated with nutritional or developmental dependency of its host cell or organism. We have found a mycetomic Rickettsia that is a strict obligatory symbiont of the parthenogenetic booklouse Liposcelis bostrychophila (Psocoptera). These rickettsiae show an evolutionary transition from a solitary to a primary mycetomic bacterium adapted to the development of its host. These intracellular and intranuclear bacteria reside in specialized cells in several tissues. Their distribution changes markedly with the development of their host. The most advanced phenotype is a paired mycetome in the abdomen, described for the first time for Rickettsia and this host order. The mycetomic rickettsiae of two parthenogenetic book lice species are in the spotted fever group and in the basal limoniae group. While mycetomic bacteria are well known for their metabolic or light-emitting functions, these rickettsiae have an essential role in the early development of the oocyte. Removal of the Rickettsia stops egg production and reproduction in the book louse. In two phylogenetically distant psocopteran species, Rickettsia are shown to be associated with four transitional stages from free bacteria, infected cells, through single mycetocytes to organ-forming mycetomes. PMID:17012243

Perotti, M Alejandra; Clarke, Heather K; Turner, Bryan D; Braig, Henk R

2006-11-01

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Rickettsia parkeri in Amblyomma triste from Uruguay  

OpenAIRE

Our goal was to detect whether spotted fever group Rickettsia are found in the suspected vector of rickettsioses, Amblyomma triste, in Uruguay. Rickettsia parkeri was detected in A. triste, which suggests that this species could be considered a pathogenic agent responsible for human rickettsioses in Uruguay.

Venzal, Jose? M.; Portillo, Ara?nzazu; Estrada-pen?a, Agusti?n; Castro, Oscar; Cabrera, Perla A.; Oteo, Jose? A.

2004-01-01

29

Spotted fever group rickettsiae in ticks, Germany.  

Science.gov (United States)

To explore increased risk for human Rickettsia spp. infection in Germany, we investigated recreational areas and renatured brown coal surface-mining sites (also used for recreation) for the presence of spotted fever group rickettsiae in ticks. R. raoultii (56.7%), R. slovaca (13.3%), and R. helvetica (>13.4%) were detected in the respective tick species. PMID:21529404

Silaghi, Cornelia; Hamel, Dietmar; Thiel, Claudia; Pfister, Kurt; Pfeffer, Martin

2011-05-01

30

Spotted Fever Group Rickettsiae in Ticks, Germany  

OpenAIRE

To explore increased risk for human Rickettsia spp. infection in Germany, we investigated recreational areas and renatured brown coal surface-mining sites (also used for recreation) for the presence of spotted fever group rickettsiae in ticks. R. raoultii (56.7%), R. slovaca (13.3%), and R. helvetica (>13.4%) were detected in the respective tick species.

Silaghi, Cornelia; Hamel, Dietmar; Thiel, Claudia; Pfister, Kurt; Pfeffer, Martin

2011-01-01

31

Caracterización de las células ganglionares de la retina melanopsínicas y estudio de la degeneración y neuroprotección de las células ganglionares de la retina en el roedor  

OpenAIRE

Objetivos Caracterizar la población de células ganglionares de la retina melanopsínicas (CGRm) en la rata adulta en relación con la población total de células ganglionares de la retina (CGR) y analizar su distribución espacial en la retina. Caracterizar si el Brn3a es un buen marcador de las CGR en ratones albino (Swiss) y pigmentado (C57BL/6). Analizar la curva temporal de muerte de las CGR de ratón después de la sección del nervio óptico (SNO), y evaluar el efecto neuropro...

Galindo Romero, Caridad

2014-01-01

32

Proteomic Analysis of Rickettsia parkeri Strain Portsmouth?  

OpenAIRE

Rickettsia parkeri, a recently recognized pathogen of human, is one of several Rickettsia spp. in the United States that causes a spotted fever rickettsiosis. To gain insights into its biology and pathogenesis, we applied the proteomics approach to establish a two-dimensional gel proteome reference map and combined this technique with cell surface biotinylation to identify surface-exposed proteins of a low-passage isolate of R. parkeri obtained from a patient. We identified 91 proteins by mat...

Pornwiroon, Walairat; Bourchookarn, Apichai; Paddock, Christopher D.; Macaluso, Kevin R.

2009-01-01

33

PCR MÚLTIPLE ANIDADA PARA DETECCIÓN DE FITOPLASMAS Y RICKETTSIA ASOCIADOS CON LOS SÍNTOMAS DEL COGOLLO ARREPOLLADO (BTS) EN PAPAYO / NESTED PCR MULTIPLEX FOR THE DETECTION OF THE PHYTOPLASMAS AND RICKETTSIA ASSOCIATED WITH BUNCHY TOP SYMPTOM (BTS) IN PAPAYO  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Recientemente, los grupos fitoplasmas 16SrI «Candidatus Phytoplasma asteris»,16SrII «Candidatus Phytoplasma aurantifolia» y rickettsia se asociaron a síntomas del cogollo arrepollado (BTS) del papayo en Cuba. El ADN de muestras de plantas de papayo positivas a fitoplasmas y rickettsia se empleó para [...] optimizar y evaluar un ensayo de PCR múltiple anidada. Se usaron los iniciadores de PCR genéricos para fitoplamas R16mF2/R16mR1en la primera amplificación y en la segunda amplificación una mezcla PBTF1/PBTR1 (específicos para rickettsia) y R16F2n/BPVNr/p86r (específicos para los grupos de fitoplasmas 16SrI y 16SrII). El ensayo de diagnóstico por PCR múltiple anidado permitió la detección simultánea de fitoplasmas y rickettsia en muestras de plantas de papayo de condiciones de campo. Abstract in english Recently, the phytoplasmas groups 16SrI `Candidatus Phytoplasma aurantifolia' and 16SrII group `Candidatus Phytoplasma asteris' and rickettsia were associated with Bunchy Top Symptoms (BTS) of papayo in Cuba. The ADN samples from papaya plants positive to phytoplasma and rickettsia were used for eva [...] luate and optimize nPCR multiplex. Generic primers for amplification of phytoplasma R16mF2/R16mR1 were used in the first reaction. A mixture composed of PBTF1/PBTR1and R16F2n/BPVNr/p86r for rickettsia and phytoplasma (16SrI and 16SrII groups) amplifications were used in the second reaction. The diagnostic assay by the nPCR multiplex permitted the simultaneous detection of phytoplasmas and rickettsias in samples of papaya plants from the field.

K, Acosta; Y, Martínez; L, Zamora; A, Fernández; M.E, Santos-Cervantes; N.E, Leyva-López.

2011-12-01

34

Rickettsia typhi and Rickettsia felis in Xenopsylla cheopis and Leptopsylla segnis Parasitizing Rats in Cyprus  

OpenAIRE

Fleas collected from rats during a three-year period (2000–2003) in 51 areas of all provinces of Cyprus were tested by molecular analysis to characterize the prevalence and identity of fleaborne rickettsiae. Rickettsia typhi, the causative agent of murine typhus, was detected in Xenopsylla cheopis (4%) and in Leptopsylla segnis (6.6%). Rickettsia felis was detected in X. cheopis (1%). This is the first report of R. typhi in X. cheopis and L. segnis from rats, in Cyprus, and the first report...

Christou, Christos; Psaroulaki, Anna; Antoniou, Maria; Toumazos, Pavlos; Ioannou, Ioannis; Mazeris, Apostolos; Chochlakis, Dimosthenis; Tselentis, Yannis

2010-01-01

35

Detección molecular de Rickettsia massiliae y Anaplasma platys en garrapatas Rhipicephalus sanguineus y caninos domésticos del municipio de Bahía Blanca (Argentina) / Molecular detection of Rickettsia massiliae and Anaplasma platys infecting Rhipicephalus sanguineus ticks and dogs, Bahía Blanca (Argentina)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: Las rickettsiosis, ehrlichiosis y anaplasmosis son causadas por bacterias gramnegativas, intracelulares obligadas y transmitidas principalmente por artrópodos. Objetivo: Detectar y caracterizar molecularmente estos patógenos en garrapatas y caninos domésticos del municipio de Bahía Bla [...] nca (provincia de Buenos Aires, Argentina). Material y Métodos: Se estudiaron 56 muestras sanguíneas de caninos, 75 garrapatas Rhipicephalus sanguineus y 7 Amblyomma tigrinum. Las muestras fueron analizadas por RPC para Rickettsia (espacio intergénico 23S-5S ARNr), Ehrlichia y Anaplasma (16S ARNr), y Anaplasma platys (16S ARNr). Resultados: Se detectó positividad a Rickettsia en 12% de R. sanguineus, identificándose por secuenciación a Rickettsia massiliae. Las muestras sanguíneas de los caninos resultaron en 37,5% positivas a A. platys. También se caracterizaron molecularmente por la amplificación del fragmento del gen citrato sintasa (gltA) (género Rickettsia) y del gen groESL (A. platys). Se construyeron árboles filogenéticos utilizando el método del vecino más cercano (neighbor-joining) revelando que las secuencias obtenidas son similares a las de otras regiones geográficas. Conclusión: Los resultados indican la presencia de R. massiliae en garrapatas R. sanguineus en una segunda zona urbana de Sudamérica y la infección por A. platys en caninos, siendo la región más austral de Argentina donde ha sido notificada. Abstract in english Background: Rickettsioses, ehrlichioses and anaplasmoses are caused by Gram negative obligate intracellular bacteria and transmitted mainly by arthropods. Aim: To detect and perform the molecular characterization of these pathogens in ticks and domestic dogs in Bahia Blanca City (Buenos Aires, Argen [...] tina). Methods: Fifty six blood samples from dogs and 82 ticks (75 Rhipicephalus sanguineus and 7 Amblyomma tigrinum) were studied. The samples were analyzed by PCR for Rickettsia (intergenic space 23S-5S rRNA), Ehrlichia/Anaplasma (16S rRNA), and Anaplasma platys (16S rRNA). Results: 12% of R. sanguineus resulted positive for Rickettsia, identified by sequencing as Rickettsia massiliae; and 37.5% of the canine blood samples analyzed were positive for A. platys. Molecular characterization was also performed by amplification of the fragment of the citrate synthase gene (gltA) (Rickettsia genus) and the groESL gene (A. platys). Phylogenetic trees were constructed using the neighbor-joining method. These trees revealed that sequences obtained are similar to those from other geographical regions. Conclusion: The results indicate the presence of R. massiliae in R. sanguineus ticks for the second time in an urban area of South America and A. platys infection in dogs, being the southernmost region of Argentina where it has been notified.

Gabriel L, Cicuttin; Pablo, Vidal; M, Nazarena De Salvo; Fernando J, Beltrán; Federico E, Gury Dohmen.

2014-10-01

36

Isolamento de Rickettsia em cultura de células vero  

Directory of Open Access Journals (Sweden)

Full Text Available Embora o diagnóstico da febre maculosa baseie-se em sinais e sintomas característicos, o mesmo requer confirmação laboratorial, pois existem alguns diagnósticos diferenciais possíveis como meningococcemia, leptospirose, infecção por enterovírus e febre tifóide. A confirmação laboratorial pode ser feita através da pesquisa de anticorpos específicos, possível somente alguns dias após o aparecimento da doença, através do isolamento do agente em amostras de sangue e/ou biópsia de pele, e ainda, de amostras de carrapatos coletados do paciente ou de animais reservatório. O isolamento a partir de sangue ou biópsia de pele resulta em diagnóstico precoce da doença, pois na fase de rickettsemia ainda não há anticorpos detectáveis no sangue. Assim, com o objetivo de facilitar o diagnóstico precoce da febre maculosa, estabelecemos um método de isolamento de rickettsia em cultura de células vero. Para a padronização foi inoculada amostra padrão de Rickettsia rickettsii, cepa Sheyla Smith, cedida pelo CDC. A identificação foi feita através da reação de imunofluorescência indireta. A presença de microrganismos verdes fluorescentes visualizados no interior do citoplasma das células caracterizou o crescimento do agente. Posteriormente, a metodologia foi confirmada pelo isolamento do agente da febre maculosa em amostras de biópsia de pele de paciente proveniente de área endêmica no Estado de São Paulo, bem como, de amostras de carrapato do gênero Amblyomma, considerado o reservatório e transmissor da doença no Brasil.

Melles Heloisa Helena Barbosa

1999-01-01

37

Identificación de Rickettsia andeanae en dos regiones de Chile / Detection of Rickettsia andeanae in two regions of Chile  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: Candidatus 'Rickettsia andeanae', especie de reciente reconocimiento y rol patógeno no precisado, ha sido identificada en garrapatas del género Amblyomma en Perú, E.U.A. y AArgentina y recientemente en Chile, en un espécimen de Amblyomma triste de un sector rural de Arica. Objetivo: De [...] terminar presencia de agentes rickettsiales en garrapatas de perros domésticos de dos regiones de Chile. Métodos: Estudio transversal, descriptivo, en las regiones de Coquimbo y La Araucanía, con muestreo domiciliario en las ciudades de Coquimbo y AAngol y localidades rurales cercanas, en primavera-verano 2011-2012. Se examinó un perro por vivienda, recolectando garrapatas si estaban presentes; luego de su identificación taxonómica fueron sometidas a amplificación y secuenciación de los genes gltA y ompA. Resultados: Se examinaron 462 perros, 255 tenían garrapatas (55%). En las ciudades se encontró exclusivamente Rhipicephalus sanguineus; en las localidades rurales además Amblyomma tigrinum. Las secuencias obtenidas en 12 especímenes de A. tigrinum correspondieron a Candidatus 'Rickettsia andeanae'. Conclusiones: Se documenta presencia de R. andeanae en dos regiones distantes de Chile, lo que se suma a su reciente hallazgo en el extremo norte del país. Este nuevo agente rickettsial se limita a garrapatas del género Amblyomma y a zonas rurales, lo que concuerda con lo documentado en otros países y con el hábitat de esta garrapata. Abstract in english Introduction: Candidatus 'Rickettsia andeanae' is recently recognized specie, with no attributed pathogenic role so far. It has been reported in Amblyomma ticks from Peru, USA and Argentina, and recently in Chile, in one specimen of Amblyomma triste from a rural locality in Arica. Objective: To dete [...] rmine the presence of rickettsial agents in ticks from domestic dogs from two Chilean regions. Methods: A transversal descriptive study in Coquimbo and La Araucanía regions, consisted in households sampling in the cities of Coquimbo and Aingol and nearby rural localities, in spring-summer 2011-2012. One dog per house was examined and ticks were collected when present; after taxonomic identification, gltA and ompA genes were amplified and sequenced. Results: 462 dogs were examined, 255 had ticks (55%). In the cities just Rhipicephalus sanguineus was found; in rural localities also Amblyomma tigrinum. Obtained sequences in 12 specimens from A. tigrinum corresponded to Candidatus 'Rickettsia andeanae'. Conclusions: We documented the presence of R. andeanae in two distant Chilean regions, which is added to its recently finding in the Northern extreme of the country. This new rickettsial agent limits to Amblyomma ticks and rural areas, as it was reported in other American countries and corresponds with this tick habitat.

Katia, Abarca; Javier, López; Gerardo, Acosta-Jamett; Constanza, Martinez-Valdebenito.

2013-08-01

38

Molecular detection of Rickettsia felis and Candidatus Rickettsia Asemboensis in Fleas from Human Habitats, Asembo, Kenya  

OpenAIRE

The flea-borne rickettsioses murine typhus (Rickettsia typhi) and flea-borne spotted fever (FBSF) (Rickettsia felis) are febrile diseases distributed among humans worldwide. Murine typhus has been known to be endemic to Kenya since the 1950s, but FBSF was only recently documented in northeastern (2010) and western (2012) Kenya. To characterize the potential exposure of humans in Kenya to flea-borne rickettsioses, a total of 330 fleas (134 pools) including 5 species (Xenopsylla che...

Jiang, Ju; Maina, Alice N.; Knobel, Darryn Leslie; Cleaveland, Sarah; Laudisoit, Anne; Wamburu, Kabura; Ogola, Eric; Parola, Philippe; Breiman, Robert F.; Njenga, M. Kariuki; Richards, Allen L.

2013-01-01

39

Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia / Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp. [...] , Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la técnica de ELISA indirecta. Para la determinación de Rickettsia sp. y Ehrlichia sp. se uso la técnica de inmunofluorescencia indirecta. Resultados. La población evaluada estaba compuesta por 27 (30%) ordeñadores, 21 (23%) jornaleros, 18 (20%) profesionales del campo y 24 (27%) que realizaban otras actividades. Ventidós (24%) muestras resultaron positivas en alguna de las pruebas. De éstas, 12 (13,3%) fueron positivas para Leptospira sp., 7 (7,8%) para Rickettsia sp. y 3 (3,3%) ara Ehrlichia sp. Conclusión. Este fue el primer estudio que se llevó a cabo en el departamento de Sucre y permitió demostrar que existe una prevalencia importante de Leptospira p.,Rickettsia sp. y Ehrlichia sp.. Los factores de riesgo ocupacional fueron factores determinantes en la seropositividad. Abstract in english Objective. To determine the seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in agricultural workers of Sucre. Methods. A descriptive prospective cross-sectional study was conducted in ninety rural workers of Sucre. Presence of serum antibodies anti-IgM specific anti-Leptospira by [...] indirect ELISA was established. For the determination of Rickettsia and Ehrlichia indirect inmunoflorescence was used. Results.The population was composed by 27 (30%) milkers, 21 (23%) day workers, 18 farm professionals (20%) and 24 (26%) workers in others activities. A total of 22 (24%) samples were positive to some test. Twelve (13.3%) were positive to Leptospira sp., seven (7.8%) to Rickettsia sp. and three (3.3%) o Ehrlichia sp.. Conclusions. This is the first study carried out in Sucre; there is an important prevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp..The occupational risk factors were decisive in the seropositivity.

Rodrigo, Ríos; Sila, Franco; Salim, Mattar; Mary, Urrea; Vaneza, Tique.

2008-06-01

40

Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia  

Directory of Open Access Journals (Sweden)

Full Text Available Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp., Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la técnica de ELISA indirecta. Para la determinación de Rickettsia sp. y Ehrlichia sp. se uso la técnica de inmunofluorescencia indirecta. Resultados. La población evaluada estaba compuesta por 27 (30% ordeñadores, 21 (23% jornaleros, 18 (20% profesionales del campo y 24 (27% que realizaban otras actividades. Ventidós (24% muestras resultaron positivas en alguna de las pruebas. De éstas, 12 (13,3% fueron positivas para Leptospira sp., 7 (7,8% para Rickettsia sp. y 3 (3,3% ara Ehrlichia sp. Conclusión. Este fue el primer estudio que se llevó a cabo en el departamento de Sucre y permitió demostrar que existe una prevalencia importante de Leptospira p.,Rickettsia sp. y Ehrlichia sp.. Los factores de riesgo ocupacional fueron factores determinantes en la seropositividad.Objective. To determine the seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in agricultural workers of Sucre. Methods. A descriptive prospective cross-sectional study was conducted in ninety rural workers of Sucre. Presence of serum antibodies anti-IgM specific anti-Leptospira by indirect ELISA was established. For the determination of Rickettsia and Ehrlichia indirect inmunoflorescence was used. Results.The population was composed by 27 (30% milkers, 21 (23% day workers, 18 farm professionals (20% and 24 (26% workers in others activities. A total of 22 (24% samples were positive to some test. Twelve (13.3% were positive to Leptospira sp., seven (7.8% to Rickettsia sp. and three (3.3% o Ehrlichia sp.. Conclusions. This is the first study carried out in Sucre; there is an important prevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp..The occupational risk factors were decisive in the seropositivity.

Rodrigo Ríos

2008-06-01

41

First report on the occurrence of Rickettsia slovaca and Rickettsia raoultii in Dermacentor silvarum in China  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Rickettsioses are among both the longest known and most recently recognized infectious diseases. Although new spotted fever group rickettsiae have been isolated in many parts of the world including China, Little is known about the epidemiology of Rickettsia pathogens in ticks from Xinjiang Autonomous Region of China. Methods In an attempt to assess the potential risk of rickettsial infection after exposure to ticks in Xinjiang Uygur Autonomous Region of China, a total of 200 Dermacentor silvarum ticks collected in Xinyuan district were screened by polymerase chain reaction based on the outer membrane protein A gene. Results 22 of the 200 specimens (11% were found to be positive by PCR. Phylogenetic analysis of OmpA sequences identified two rickettsial species, Rickettsia raoultii (4.5% and Rickettsia slovaca (6.5%. Conclusions This study has reported the occurrence of Rickettsia raoultii and Rickettsia slovaca in Xinjiang Autonomous Region of China and suggests that Dermacentor silvarum could be involved in the transmission of rickettsial agents in China. Further studies on the characterization and culture of rickettsial species found in Dermacentor silvarum should be performed to further clarify this. Additionally, the screening of human specimens for rickettsial disease in this region will define the incidence of infection.

Tian Zhan-Cheng

2012-01-01

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Bartonella quintana and Rickettsia felis in Gabon  

OpenAIRE

We detected Rickettsia felis DNA in Ctenocephalides felis and Bartonella quintana DNA in 3 Pulex irritans fleas taken from a pet Cercopithecus cephus monkey in Gabon, sub-Saharan Africa. This is the first report of B. quintana in the human flea.

Rolain, Jean-marc; Bourry, Olivier; Davoust, Bernard; Raoult, Didier

2005-01-01

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Rickettsia felis in Fleas, Western Australia  

OpenAIRE

This study is the first confirmation of Rickettsia felis in Australia. The organism was identified from 4 species of fleas obtained from dogs and cats in Western Australia, by using polymerase chain reaction amplification and DNA sequencing of the citrate synthase and outer membrane protein A genes.

Schloderer, Drew; Owen, Helen; Clark, Phillip; Stenos, John; Fenwick, Stanley G.

2006-01-01

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Rickettsia felis in Xenopsylla cheopis, Java, Indonesia  

OpenAIRE

Rickettsia typhi and R. felis, etiologic agents of murine typhus and fleaborne spotted fever, respectively, were detected in Oriental rat fleas (Xenopsylla cheopis) collected from rodents and shrews in Java, Indonesia. We describe the first evidence of R. felis in Indonesia and naturally occurring R. felis in Oriental rat fleas.

Jiang, Ju; Soeatmadji, Djoko W.; Henry, Katherine M.; Ratiwayanto, Sutanti; Bangs, Michael J.; Richards, Allen L.

2006-01-01

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In Vitro Activities of Telithromycin (HMR 3647) against Rickettsia rickettsii, Rickettsia conorii, Rickettsia africae, Rickettsia typhi, Rickettsia prowazekii, Coxiella burnetii, Bartonella henselae, Bartonella quintana, Bartonella bacilliformis, and Ehrlichia chaffeensis  

OpenAIRE

In vitro activities of telithromycin compared to those of erythromycin against Rickettsia spp., Bartonella spp., Coxiella burnetii, and Ehrlichia chaffeensis were determined. Telithromycin was more active than erythromycin against Rickettsia, Bartonella, and Coxiella burnetii, with MICs of 0.5 ?g/ml, 0.003 to 0.015 ?g/ml, and 1 ?g/ml, respectively, but was inactive against Ehrlichia chaffeensis.

Rolain, Jean-marc; Maurin, Max; Bryskier, Andre?; Raoult, Didier

2000-01-01

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Antibodies against rickettsiae from spotted fever groups in horses from two mesoregions in the state of Santa Catarina, Brazil / Anticorpos contra rickettsias do grupo da febre maculosa em equinos de duas mesorregiões de Santa Catarina, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Bactérias do gênero Rickettsia são agentes da Febre Maculosa Brasileira (FMB), uma doença zoonótica, de difícil diagnóstico, rápida evolução e que pode levar o indivíduo à morte. Anticorpos contra Rickettsia spp. em equinos foram pesquisados, por meio da Reação de Imunofluorescência Indireta (RIFI?6 [...] 4), em 150 amostras de sangue colhidas de animais em duas mesorregiões de Santa Catarina (Planalto Serrano e Vale do Itajaí). A ocorrência de anticorpos contra Rickettsia spp. observada em equinos de duas mesorregiões de Santa Catarina foi de 18,66%, ocorrendo reações cruzadas em todas as amostras positivas para, no mínimo, duas das espécies testadas. Isoladamente, de acordo com as espécies, 25 (16,66%) amostras foram positivas para R. rickettsii, 15 (10%) para R. parkeri, 22 (14,66%) para R. amblyommii, 23 (15,33%) para R. rhipicephali, 16 (10,66%) para R. bellii e 19 (12,66%) para R. felis. Somente dois animais resultaram em um sorodiagnóstico conclusivo, um para Rickettsia bellii e outro para R. rickettsii, nas diluições máximas de 1:4096 e 1:512, respectivamente. A ocorrência de anticorpos contra Rickettsia spp. em equinos de duas mesorregiões de Santa Catarina indica a circulação de agentes da FMB nestes animais sentinela e ratificam a importância do estudo da febre maculosa no estado de Santa Catarina. Abstract in english Bacteria of the Rickettsia genus are agents of Brazilian Spotted Fever (BSF), a zoonotic disease which is difficult to diagnose, evolves quickly and can result in death. Antibodies against Rickettsia spp. in horses were studied, by means of Indirect Immunofluorescence Assay (IFAT ?64), in 150 blood [...] samples taken from animals in two Santa Catarina mesoregions (Planalto Serrano and Vale do Itajaí). The overall occurrence of Rickettsia spp. antibodies in horses was 18.66%, with cross-reactivity occurring in all positive samples for at least two of the species tested. Separately, according to the species, 25 (16.66%) samples were positive for R. rickettsii, 15 (10%) for R. parkeri, 22 (14.66%) for R. amblyommii, 23 (15.33%) for R. rhipicephali, 16 (10.66%) for R. bellii and 19 (12.66%) for R. felis. Only two animals resulted in a conclusive serodiagnosis, one for R. bellii and the other for R. rickettsii, at maximum dilutions of 1:4096 and 1:512, respectively. The occurrence of antibodies against Rickettsia spp. in horses from two mesoregions in the state of Santa Catarina indicates the movement of BSF agents in these sentinel animals and confirms the importance of studying spotted fever in the state of Santa Catarina.

A.P., Medeiros; A.B., Moura; A.P., Souza; V., Bellato; A.A., Sartor; A., Vieira-Neto; J., Moraes-Filho; M.B., Labruna.

1713-17-01

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Race and Rickettsiae: A United States Perspective  

OpenAIRE

US surveillance programs for Rocky Mountain spotted fever (RMSF), ehrlichiosis, and anaplasmosis collect demographic data on patients, including race and ethnicity. Reporting of these diseases among race groups is not uniform across the United States. Because a laboratory confirmation is required to meet the national surveillance case definition, reporting may be influenced by a patient's access to healthcare. Determining the association between race and ethnicity with incidence of rickettsia...

Dahlgren, F. Scott; Moonesinghe, Ramal; Mcquiston, Jennifer H.

2011-01-01

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Detection of Rickettsia in Rhipicephalus sanguineus Ticks and Ctenocephalides felis Fleas from Southeastern Tunisia by Reverse Line Blot Assay  

OpenAIRE

Ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern Tunisia were screened for Rickettsia infection using reverse line blot assay. Evidence of spotted fever group Rickettsia was obtained. We detected Rickettsia felis in fleas, Rickettsia massiliae Bar 29 and the Rickettsia conorii Israeli spotted fever strain in ticks, and Rickettsia conorii subsp. conorii and Rickettsia spp. in both arthropods. The sensitivity of the adopted technique allowed the identificati...

Khrouf, Fatma; M Ghirbi, Youmna; Znazen, Abir; Ben Jemaa, Mounir; Hammami, Adnene; Bouattour, Ali

2014-01-01

49

High Seroprevalence for Typhus Group Rickettsiae, Southwestern Tanzania  

OpenAIRE

Rickettsioses caused by typhus group rickettsiae have been reported in various African regions. We conducted a cross-sectional survey of 1,227 participants from 9 different sites in the Mbeya region, Tanzania; overall seroprevalence of typhus group rickettsiae was 9.3%. Risk factors identified in multivariable analysis included low vegetation density and highway proximity.

Dill, Tatjana; Dobler, Gerhard; Saathoff, Elmar; Clowes, Petra; Kroidl, Inge; Ntinginya, Elias; Machibya, Harun; Maboko, Leonard; Lo?scher, Thomas; Hoelscher, Michael; Heinrich, Norbert

2013-01-01

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Infection of Ixodes scapularis ticks with Rickettsia monacensis expressing green fluorescent protein: A model system  

OpenAIRE

Ticks (Acari:Ixodidae) are ubiquitous hosts of rickettsiae (Rickettsiaceae:Rickettsia), obligate intracellular bacteria that occur as a continuum from nonpathogenic arthropod endosymbionts to virulent pathogens of both arthropod vectors and vertebrates. Visualization of rickettsiae in hosts has traditionally been limited to techniques utilizing fixed tissues. We report epifluorescence microscopy observations of unfixed tick tissues infected with a spotted fever group endosymbiont, Rickettsia ...

Baldridge, Gerald D.; Kurtti, Timothy J.; Burkhardt, Nicole; Baldridge, Abigail S.; Nelson, Curtis M.; Oliva, Adela S.; Munderloh, Ulrike G.

2007-01-01

51

Serological cross-reaction and cross-protection in guinea pigs infected with Rickettsia rickettsii and Rickettsia montana.  

OpenAIRE

Antisera produced in guinea pigs inoculated with Rickettsia rickettsii or Rickettsia montana were cross-reactive but sufficiently specific to identify the primary infecting agent. Guinea pigs immunized with R. montana were protected from fatal infection with R. rickettsii, although a few (25%) developed mild fever of short duration.

Feng, W. C.; Waner, J. L.

1980-01-01

52

Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes / Células Ganglionares Apoptóticas en la Capa de la Retina de Crías de Ratas Debido a la Diabetes Gestacional  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Estudios previos en un modelo animal han demostrado los efectos adversos de la diabetes gestacional en la densidad neuronal del hipocampo. El objetivo fue determinar el efecto de la diabetes gestacional en la densidad de las células ganglionares de la retina, en el espesor de la capa de la retina y [...] en la densidad de las células apoptóticas ganglionares, en crías de ratas de 28 días. En este estudio experimental, 10 ratas Wistar fueron asignadas aleatoriamente en grupos control y diabéticos. La diabetes gestacional se indujo a partir de la administración de 40 mg/kg/peso corporal de estreptozotocina en el primer día de la gestación, por vía intraperitoneal. Al grupo control se administró un volumen equivalente de solución salina normal. En el día 28 luego del nacimiento, se seleccionaron aleatoriamente seis crías procedentes de los grupos con diabetes gestacional y controles, se eutanasiaron y se tomaron muestras de los ojos, en forma de secciones de 6 micrómetros, las cuales se tiñeron con H & E. La densidad de las células ganglionares y el número final de células dUTP positivas (TUNEL) se evaluaron a nivel de la capa ganglionar de la retina, en un área de 20.000 mm2. La densidad de las células ganglionares se redujo un 27,4% en la descendencia con diabetes gestacional en comparación con los controles (22,5±1,5 vs. 31,0±0,9, P Abstract in english Previous studies have shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal models. This study was conducted to determine the effect of gestational diabetes on retinal ganglionic cell density, the thickness of the retinal layer and apoptotic ganglionic cell dens [...] ity in 28-day-old of rat offspring. In this experimental study, 10 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally, dams in control group received an equivalent volume normal saline. At postnatal day 28, six offspring of each gestational diabetes and controls were randomly selected, sacrificed and sections (6 micrometer) were taken from the eye and stained with hematoxylin and eosin. The density of ganglionic cells and the number of dUTP end-labeling (TUNEL)-positive cells were evaluated in 20000 mm2 area of ganglion layer of the retina. The ganglionic cells density were reduced (27.4%) in gestational diabetic offspring in compared to controls (22.5±1.5 vs. 31.0±0.9, P

Simin, Najafdari; Noorallah, Rezaei; Majid Malekzadeh, Shafaroodi; Soraya, Ghafari; Mohammad Jafar, Golalipour.

1131-11-01

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Neuroprotección de las células ganglionares de la retina / Retinal ganglion cell neuroprotection in culture  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Objetivo: Estudiar la supervivencia de las células ganglionares de la retina (CGR) de cerdo en cultivo, analizando el posible efecto neuroprotector de las células de Müller de la retina (CMR) y del factor neurotrófico derivado del cerebro (BDNF). Métodos: Las retinas de cerdo adulto fueron disociada [...] s y cultivadas en diferentes condiciones: 1) sobre sustrato de laminina/poli-D-lisina en medio de cultivo químicamente definido; 2) sobre sustrato de laminina/poli-D-lisina en medio químicamente definido al que se añadió BDNF; 3) sobre monocapas de CMR en medio químicamente definido; 4) sobre sustrato de laminina/poli-D-lisina en medio condicionado procedente del sobrenadante de las CMR. Las CGR fueron identificadas mediante inmunocitoquímica, utilizando anticuerpos contra el neurofilamento de 68 kDa, y observadas con un microscopio de fluorescencia. Se analizó la supervivencia para cada condición de cultivo y se clasificaron las CGR en función de su tamaño y del número y longitud de las neuritas. Resultados: La supervivencia de las CGR aumentó cuando las células fueron cultivadas sobre monocapas confluentes de CMR o en medio condicionado. Estas condiciones produjeron un incremento en el área media de las células y un aumento en el número de neuritas emitidas por cada célula, así como en la longitud de las neuritas. Cuando el medio de cultivo se suplementó con BDNF no se obtuvo ningún efecto sobre la supervivencia de las CGR aunque aumentó el tamaño, y el número y longitud de sus neuritas. Conclusión: Nuestro trabajo demuestra que algún/os factor/es secretados por las células de Müller tienen un efecto neuroprotector sobre las CGR in vitro. El BDNF produce también un incremento en el área media de las células y favorece la formación de neuritas, sin embargo no aumenta la supervivencia. Abstract in english Purpose: To study the pig retinal ganglion cell (RGC) survival in culture, analysing the possible neuroprotective effect of retinal Müller glia (RMG) and brain-derived neurotrophic factor (BDNF). Methods: Adult pig retina were dissociated and cultured under different conditions: 1) on laminin/poly-D [...] -lysine-coated coverslips in chemically defined medium (CDM); 2) on laminin/poly-D-lysine-coated coverslips in CMD supplemented with BDNF; 3) on confluent monolayer cultures of RMG in CDM; 4) on laminin/poly-D-lysine substrate in conditioned medium obtained from RMG. RGCs were identified by immunocytochemistry using antibody against 68 kDa neurofilament and observed under an fluorescent microscope. RGCs were classified on the basis of the size, number and length of neurites, and their survival was assayed for each treatment. Results: Confluent RMG substrates and RMG conditioned medium significantly increased the survival of cultured pig RGC. Moreover these two conditions increased the mean area of RGCs and enhanced neurite growth and elongation. Addition of BDNF to culture medium did not modify survival but increased RGC size, neurite number and neurite length. Conclusions: These findings demonstrate that factor(s) secreted by RMG exert beneficial effects on adult RGC survival and neurite regeneration in vitro, and might constitute important agent(s) for RGC neuroprotection. BDNF also increases the mean area of RGCs and enhances neurite growth but it does not increase the survival of RGCs.

M, García; J, Ruiz Ederra; E, Hernández Barbáchano; JA, Urcola; J, Bilbao; J, Araiz; JA, Durán; E, Vecino.

2003-03-01

54

Neuroprotección de las células ganglionares de la retina Retinal ganglion cell neuroprotection in culture  

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Full Text Available Objetivo: Estudiar la supervivencia de las células ganglionares de la retina (CGR de cerdo en cultivo, analizando el posible efecto neuroprotector de las células de Müller de la retina (CMR y del factor neurotrófico derivado del cerebro (BDNF. Métodos: Las retinas de cerdo adulto fueron disociadas y cultivadas en diferentes condiciones: 1 sobre sustrato de laminina/poli-D-lisina en medio de cultivo químicamente definido; 2 sobre sustrato de laminina/poli-D-lisina en medio químicamente definido al que se añadió BDNF; 3 sobre monocapas de CMR en medio químicamente definido; 4 sobre sustrato de laminina/poli-D-lisina en medio condicionado procedente del sobrenadante de las CMR. Las CGR fueron identificadas mediante inmunocitoquímica, utilizando anticuerpos contra el neurofilamento de 68 kDa, y observadas con un microscopio de fluorescencia. Se analizó la supervivencia para cada condición de cultivo y se clasificaron las CGR en función de su tamaño y del número y longitud de las neuritas. Resultados: La supervivencia de las CGR aumentó cuando las células fueron cultivadas sobre monocapas confluentes de CMR o en medio condicionado. Estas condiciones produjeron un incremento en el área media de las células y un aumento en el número de neuritas emitidas por cada célula, así como en la longitud de las neuritas. Cuando el medio de cultivo se suplementó con BDNF no se obtuvo ningún efecto sobre la supervivencia de las CGR aunque aumentó el tamaño, y el número y longitud de sus neuritas. Conclusión: Nuestro trabajo demuestra que algún/os factor/es secretados por las células de Müller tienen un efecto neuroprotector sobre las CGR in vitro. El BDNF produce también un incremento en el área media de las células y favorece la formación de neuritas, sin embargo no aumenta la supervivencia.Purpose: To study the pig retinal ganglion cell (RGC survival in culture, analysing the possible neuroprotective effect of retinal Müller glia (RMG and brain-derived neurotrophic factor (BDNF. Methods: Adult pig retina were dissociated and cultured under different conditions: 1 on laminin/poly-D-lysine-coated coverslips in chemically defined medium (CDM; 2 on laminin/poly-D-lysine-coated coverslips in CMD supplemented with BDNF; 3 on confluent monolayer cultures of RMG in CDM; 4 on laminin/poly-D-lysine substrate in conditioned medium obtained from RMG. RGCs were identified by immunocytochemistry using antibody against 68 kDa neurofilament and observed under an fluorescent microscope. RGCs were classified on the basis of the size, number and length of neurites, and their survival was assayed for each treatment. Results: Confluent RMG substrates and RMG conditioned medium significantly increased the survival of cultured pig RGC. Moreover these two conditions increased the mean area of RGCs and enhanced neurite growth and elongation. Addition of BDNF to culture medium did not modify survival but increased RGC size, neurite number and neurite length. Conclusions: These findings demonstrate that factor(s secreted by RMG exert beneficial effects on adult RGC survival and neurite regeneration in vitro, and might constitute important agent(s for RGC neuroprotection. BDNF also increases the mean area of RGCs and enhances neurite growth but it does not increase the survival of RGCs.

M García

2003-03-01

55

Variations of Plasmid Content in Rickettsia felis  

Science.gov (United States)

Background Since its first detection, characterization of R. felis has been a matter of debate, mostly due to the contamination of an initial R. felis culture by R. typhi. However, the first stable culture of R. felis allowed its precise phenotypic and genotypic characterization, and demonstrated that this species belonged to the spotted fever group rickettsiae. Later, its genome sequence revealed the presence of two forms of the same plasmid, physically confirmed by biological data. In a recent article, Gillespie et al. (PLoS One. 2007;2(3):e266.) used a bioinformatic approach to refute the presence of the second plasmid form, and proposed the creation of a specific phylogenetic group for R. felis. Methodology/Principal Findings In the present report, we, and five independent international laboratories confirmed unambiguously by PCR the presence of two plasmid forms in R. felis strain URRWXCal2T, but observed that the plasmid content of this species, from none to 2 plasmid forms, may depend on the culture passage history of the studied strain. We also demonstrated that R. felis does not cultivate in Vero cells at 37°C but generates plaques at 30°C. Finally, using a phylogenetic study based on 667 concatenated core genes, we demonstrated the position of R. felis within the spotted fever group. Significance We demonstrated that R. felis, which unambiguously belongs to the spotted fever group rickettsiae, may contain up to two plasmid forms but this plasmid content is unstable. PMID:18509454

Fournier, Pierre-Edouard; Belghazi, Lokmane; Robert, Catherine; Elkarkouri, Khalid; Richards, Allen L.; Greub, Gilbert; Collyn, François; Ogawa, Motohiko; Portillo, Arantxa; Oteo, Jose A.; Psaroulaki, Anna; Bitam, Idir; Raoult, Didier

2008-01-01

56

Detection of Rickettsia felis, Rickettsia typhi, Bartonella Species and Yersinia pestis in Fleas (Siphonaptera) from Africa  

OpenAIRE

Fleas are associated with many bacterial diseases such as rickettsioses, bartonelloses and plague. These diseases may be severe, and little is known about their prevalence. Accordingly, we believe that our data shed light on the problem of unexplained fevers in tropical and subtropical African areas. Using molecular tools, we surveyed and studied selected flea-borne agents, namely Rickettsia spp. (R. felis and R. typhi), Bartonella spp. and Y. pestis, in fleas collected in Ituri (Linga and Re...

Leulmi, Hamza; Socolovschi, Cristina; Laudisoit, Anne; Houemenou, Gualbert; Davoust, Bernard; Bitam, Idir; Raoult, Didier; Parola, Philippe

2014-01-01

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Detección de Rickettsia spp. en Suero y Garrapatas de Mamíferos Silvestres en Cautiverio en Montería, Córdoba -resumen-  

Directory of Open Access Journals (Sweden)

Full Text Available Las rickettsiosis transmitidas por garrapatas son causadas por bacterias intracelulares pertenecientes al grupo de las fiebres manchadas del género Rickettsia. Estas zoonosis son algunas de las enfermedades transmitidas por vectores de más antiguo conocimiento‚ e incluyen dentro de su ciclo de transmisión diversas especies de mamíferos. No obstante‚ en Colombia existen pocos trabajos enfocados a conocer la dinámica de las rickettsiosis en animales silvestres. El objetivo de este estudio fue detectar‚ por medio de la técnica de PCR en tiempo real‚ la presencia de Rickettsia spp.‚ en muestras de suero y garrapatas colectadas en mamíferos mantenidos en cautiverio en el Centro de Atención y Valoración de Fauna Silvestre de la Corporación autónoma Regional de los Valles del Sinú y San Jorge CAV-CVS‚ entre los años 2009 y 2014. Se obtuvieron muestras de sangre de 14 felinos‚ de las especies Leopardus pardalis (10‚ Puma concolor (3 y Panthera onca (1; de 58 primates‚ correspondientes a las especies Ateles geofroyii (17‚ Alouatta seniculus (14‚ Cebus capucinus (14‚ Saguinus oedipus (6‚ Cebus albifrons (4 Aotus sp. (2 y Ateles belzebuth (1; y de otros mamíferos: Tayassu pecari (1‚ Procyon cancrivorous (1 y Cerdocyon thous (1. En cuanto a las garrapatas‚ se colectaron e identificaron individuos del género Haemaphysalis sp. en un ejemplar de Sylvilagus sp.‚ y de las especies Amblyomma ovale‚ Riphicephalus microplus y Dermacentor nitens en P. concolor. Posteriormente fueron organizadas en pool. No se encontraron muestras de suero positivas a Rickettsia spp. (0/72. Se detectó un pool de garrapatas de la especie A. ovale positivo a este microorganismo. Este es el primer reporte en Colombia de ectoparásitos de Puma concolor positivos a Rickettsia spp. Se requiere continuar con los muestreos de ectoparásitos para determinar si este microorganismo se encuentra circulante entre mamíferos silvestres en el país.

Dave Wehdeking-Hernández

2014-12-01

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Rickettsia felis in Ctenocephalides felis from Guatemala and Costa Rica.  

Science.gov (United States)

Rickettsia felis is an emerging human pathogen associated primarily with the cat flea Ctenocephalides felis. In this study, we investigated the presence of Rickettsia felis in C. felis from Guatemala and Costa Rica. Ctenocephalides felis were collected directly from dogs and cats, and analyzed by polymerase chain reaction for Rickettsia-specific fragments of 17-kDa protein, OmpA, and citrate synthase genes. Rickettsia DNA was detected in 64% (55 of 86) and 58% (47 of 81) of flea pools in Guatemala and Costa Rica, respectively. Sequencing of gltA fragments identified R. felis genotype URRWXCal(2) in samples from both countries, and genotype Rf2125 in Costa Rica. This is the first report of R. felis in Guatemala and of genotype Rf2125 in Costa Rica. The extensive presence of this pathogen in countries of Central America stresses the need for increased awareness and diagnosis. PMID:22665618

Troyo, Adriana; Álvarez, Danilo; Taylor, Lizeth; Abdalla, Gabriela; Calderón-Arguedas, Ólger; Zambrano, Maria L; Dasch, Gregory A; Lindblade, Kim; Hun, Laya; Eremeeva, Marina E; Estévez, Alejandra

2012-06-01

59

Murine and epidemic typhus rickettsiae: how close is their relationship?  

OpenAIRE

Typhus fever has occurred globally as epidemic and endemic disorders. In 1910, Brill reported a typhus-like illness which Zinsser and others determined to be recurrent epidemic typhus fever. Maxcy, in 1926, proposed rodents and fleas as reservoir and vector, respectively, of endemic typhus, which Dyer confirmed in 1930. Animals experimentally infected with epidemic typhus (Rickettsia prowazeki) are immune to murine typhus (Rickettsia typhi) and vice versa. Similar solid cross-immunity exists ...

Woodward, T. E.

1982-01-01

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Acquisition of Rickettsia felis by Cat Fleas During Feeding  

OpenAIRE

Evidence for horizontal routes of transmission for Rickettsia felis has come from detection of R. felis infection in vertebrates and multiple blood-feeding arthropods; however, infection of cat fleas, Ctenocephalides felis, during blood feeding has not been demonstrated. In this study, the ability of cat fleas to acquire R. felis through an infectious blood meal with subsequent vertical transmission was examined. Utilizing an artificial feeding system, Rickettsia-naive fleas were exposed to R...

Reif, Kathryn E.; Kearney, Michael T.; Foil, Lane D.; Macaluso, Kevin R.

2011-01-01

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Intraocular inflammation as the main manifestation of Rickettsia conorii infection  

Directory of Open Access Journals (Sweden)

Full Text Available Archimedes LD Agahan1,3, Jenice Torres1, Graciana Fuentes-Páez1, Hernan Martínez-Osorio1, Antonio Orduña2, Margarita Calonge11Ocular Immunology and Uveitis Unit, Institute of Applied Ophthalmobiology (IOBA, 2Microbiology Department, University of Valladolid, Valladolid, Spain; 3Department of Ophthalmology and Visual Sciences, University of the Philippines, Philippine General Hospital, Manila, PhilippinesObjective: To report the clinical features and management of seven cases of intraocular inflammation caused by Rickettsia infection and review published literature.Methods: Rickettsia conorii or Rickettsia spp. infection was diagnosed based on the following criteria: (1 positive serology according to the European Guidelines, (2 titer normalization after specific treatment, and (3 complete resolution of ophthalmic disease and accompanying symptoms after antibiotic therapy.Results: Seven patients were referred for uveitis of unknown etiology. All came from regions where Mediterranean spotted fever is prevalent. One patient met the European guidelines criteria for Rickettsia spp. infection, while the other six cases met the criteria for R. conorii infection. The main symptoms were visual loss, floaters, eye redness, photophobia, and ocular pain. Predominant ophthalmic signs included vasculitis, choroiditis, vitritis, and macular edema. All patients required antibiotic treatment that resulted in the remission of the infection. Doxycycline was the first choice and the only antibiotic used to treat four patients. One patient needed ciprofloxacin as a second antibiotic after not responding to doxycycline. Two patients had doxycycline as a second antibiotic after not responding primarily to sulfonamides (which had been given after 2–3 days of doxycycline gastric intolerance; one of these patients needed ciprofloxacin as a third antibiotic.Conclusion: Intraocular inflammation can occur as the main manifestation of Rickettsia conorii or Rickettsia spp. infection. It should be considered as a differential diagnosis for uveitis especially for patients living in countries where this infection is endemic in the world. Antibiotic treatment remains effective in the management of Rickettsia infection.Keywords: intraocular inflammation, Mediterranean spotted fever, Rickettsia conorii, uveitis

Orduña A

2011-09-01

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Medical expert system on fuzzy analog ganglionar lattices: explication and prospection based on sensitivity.  

Science.gov (United States)

A reasoning method to explicate the conclusions-a posteriori- and to prospect for the next test-a priori-applied to medical expert systems based on fuzzy analog ganglionar lattices is presented. This method is founded on the sensitivity criterion of the consequent respect to the antecedents. It proves to be suited for problems of any complexity, specially when they are formulated on well-established intermediate concepts, such as syndromes, clinical finding, laboratory procedures, etc., as is usually the case in medical procedures. This type of expert system uses the structural properties of the ganglionar lattice to produce explications for its conclusions at different levels of abstraction, and to an arbitrary, but fixed, explicative degree. A measure to evaluate the consequent's achieved preciseness (certainty) is also supplied. A full example of application in the diagnosis of cardiac insufficiency with hypertensive myocardiopathy is shown, and a computational implementation of this analog procedure is described. Finally, the reasoning methods for explicating and prospecting are discussed. PMID:9110277

Holzmann, C A; San Martín, M

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Detection of an undescribed Rickettsia sp. in Ixodes boliviensis from Costa Rica.  

Science.gov (United States)

Ixodes boliviensis is a tick of carnivores that is common on domestic dogs. The only Rickettsia that has been detected previously in this species is 'Candidatus Rickettsia andeanae'. We report the detection of an undescribed Rickettsia sp., named strain IbR/CRC, in I. boliviensis collected from dogs in Costa Rica. Analyses of gltA, ompA, and htrA partial sequences place Rickettsia sp. strain IbR/CRC in the group of R. monacensis, also close to an endosymbiont of Ixodes scapularis and other undescribed rickettsiae. It was not possible to isolate Rickettsia sp. strain IbR/CRC in Vero E6 or C6/36 cell lines. Isolation and further characterization of Rickettsia sp. strain IbR/CRC and the other undescribed rickettsiae are required to determine their taxonomic status and pathogenic potential. PMID:25132535

Troyo, Adriana; Moreira-Soto, Andrés; Carranza, Marco; Calderón-Arguedas, Olger; Hun, Laya; Taylor, Lizeth

2014-10-01

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Detección de Rickettsia spp. en Garrapatas de Myrmecophaga tridactyla de Vida Libre en la Sabana Inundable de Casanare‚ Colombia -resumen-  

Directory of Open Access Journals (Sweden)

Full Text Available Las bacterias del género Rickettsia son organismos con distribución mundial‚ causantes de algunas enfermedades zoonóticas de gran importancia en salud pública. Sin embargo‚ en Colombia son pocos los estudios enfocados a conocer su distribución y los vectores involucrados. El objetivo de este estudio fue detectar la presencia de Rickettsia spp.‚ en garrapatas colectadas en nueve osos palmeros (Myrmecophaga tridactyla de vida libre en el municipio de Pore‚ Casanare‚ entre los años 2013 y 2014. Los osos palmeros fueron capturados con el método de búsqueda activa‚ para posteriormente ser anestesiados utilizando un dardo con una combinación anestésica consistente en Ketamina 12 mg/kg y Xilacina 0‚5 mg/kg por vía intramuscular. Las garrapatas fueron colectadas directamente de los individuos‚ con ayuda de pinzas. En total se obtuvieron 204 ectoparásitos‚ que fueron almacenados en alcohol al 70%‚ y posteriormente fueron identificados como pertenecientes a la especie Amblyomma cajennense. Las garrapatas se organizaron en 68 pool de tres individuos y fueron analizadas por medio de la técnica de PCR en tiempo real. Se detectaron 12 pool de garrapatas positivos a este microorganismo (17‚6%. Los resultados preliminares de este estudio muestran que Rickettsia spp.‚ bacteria intracelular importante en salud pública‚ se encuentra circulante entre ectoparásitos de animales silvestres en la sabana inundable del municipio de Pore‚ Casanare. Dado que diversos estudios han reportado que los rickettsiales tienen una mayor incidencia en ambientes húmedos‚ se recomienda continuar con los estudios en mamíferos silvestres y sus garrapatas en la sabana natural inundable.

César Rojano-Bolaño

2014-12-01

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Rickettsia Symbionts Cause Parthenogenetic Reproduction in the Parasitoid Wasp Pnigalio soemius (Hymenoptera: Eulophidae)?  

OpenAIRE

Bacteria in the genus Rickettsia are intracellular symbionts of disparate groups of organisms. Some Rickettsia strains infect vertebrate animals and plants, where they cause diseases, but most strains are vertically inherited symbionts of invertebrates. In insects Rickettsia symbionts are known to have diverse effects on hosts ranging from influencing host fitness to manipulating reproduction. Here we provide evidence that a Rickettsia symbiont causes thelytokous parthenogenesis (in which mot...

Giorgini, M.; Bernardo, U.; Monti, M. M.; Nappo, A. G.; Gebiola, M.

2010-01-01

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Rickettsia felis Infection in a Common Household Insect Pest, Liposcelis bostrychophila (Psocoptera: Liposcelidae)? †  

OpenAIRE

Many species of Rickettsia are well-known mammalian pathogens transmitted by blood-feeding arthropods. However, molecular surveys are continually uncovering novel Rickettsia species, often in unexpected hosts, including many arthropods that do not feed on blood. This study reports a systematic molecular characterization of a Rickettsia infecting the psocid Liposcelis bostrychophila (Psocoptera: Liposcelidae), a common and cosmopolitan household pest. Surprisingly, the psocid Rickettsia is sho...

Behar, Adi; Mccormick, Laurie J.; Perlman, Steve J.

2010-01-01

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TRANSCRIPTION OF THE RICKETTSIA FELIS OMPA GENE IN NATURALLY INFECTED FLEAS  

OpenAIRE

Rickettsia felis is maintained transovarially in Ctenocephalides felis fleas in a widespread geographic distribution and is transmitted to humans and animals, including opossums. This rickettsia is phylogenetically a member of the spotted fever group, most closely related to Rickettsia akari and R. australis. An unusual feature of this rickettsia is that the gene for the outer membrane protein A (OmpA) is interrupted by stop codons. To determine if this putatively dying gene is expressed, mRN...

Zavala-castro, Jorge E.; Small, Melissa; Keng, Colette; Bouyer, Donald H.; Zavala-vela?zquez, Jorge; Walker, David H.

2005-01-01

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Tuberculosis ganglionar cervical: ¿Pensamos en ella, o nos sorprende? Neck node tuberculosis: Do we consider it... or does it pop up?  

Directory of Open Access Journals (Sweden)

Full Text Available Revisamos las características clínicas, diagnóstico y manejo de la tuberculosis (TB cervical, así como resaltamos su importancia por su carácter epidémico. Presentamos dos pacientes afectados por tumoraciones laterocervicales subagudas, escasa sintomatología y excelente evolución tras su diagnóstico de TB ganglionar cervical y tratamiento antibiótico. La TB es una enfermedad que en la actual sociedad globalizada, puede encontrarse prácticamente cualquier especialista, por lo que debemos mantener un alto nivel de alerta y conocerla con detalle, para poder orientar su diagnóstico y facilitar su tratamiento precoz.We review tuberculosis clinical features, diagnosis and management as well as remark the importance of its epidemic nature. Study based on 2 patients suffering from eye-catching subacute neck lumps, sparsely symptomatic and excellent evolution after neck node TB diagnosis and antibiotic therapy TB represents a disease that, given our present time globalization, may be faced by many different specialists. That is why we must be on alert and be aware of its profile, in order to guess the right diagnosis and offer therapy.

Miguel Alberto Rodríguez-Pérez

2012-04-01

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Tuberculosis ganglionar cervical: ¿Pensamos en ella, o nos sorprende? / Neck node tuberculosis: Do we consider it... or does it pop up?  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Revisamos las características clínicas, diagnóstico y manejo de la tuberculosis (TB) cervical, así como resaltamos su importancia por su carácter epidémico. Presentamos dos pacientes afectados por tumoraciones laterocervicales subagudas, escasa sintomatología y excelente evolución tras su diagnóstic [...] o de TB ganglionar cervical y tratamiento antibiótico. La TB es una enfermedad que en la actual sociedad globalizada, puede encontrarse prácticamente cualquier especialista, por lo que debemos mantener un alto nivel de alerta y conocerla con detalle, para poder orientar su diagnóstico y facilitar su tratamiento precoz. Abstract in english We review tuberculosis clinical features, diagnosis and management as well as remark the importance of its epidemic nature. Study based on 2 patients suffering from eye-catching subacute neck lumps, sparsely symptomatic and excellent evolution after neck node TB diagnosis and antibiotic therapy TB r [...] epresents a disease that, given our present time globalization, may be faced by many different specialists. That is why we must be on alert and be aware of its profile, in order to guess the right diagnosis and offer therapy.

Miguel Alberto, Rodríguez-Pérez; Fernando, Aguirre-García.

2012-04-01

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Detection of Rickettsia felis, Rickettsia typhi, Bartonella Species and Yersinia pestis in Fleas (Siphonaptera) from Africa  

Science.gov (United States)

Little is known about the presence/absence and prevalence of Rickettsia spp, Bartonella spp. and Yersinia pestis in domestic and urban flea populations in tropical and subtropical African countries. Methodology/Principal findings Fleas collected in Benin, the United Republic of Tanzania and the Democratic Republic of the Congo were investigated for the presence and identity of Rickettsia spp., Bartonella spp. and Yersinia pestis using two qPCR systems or qPCR and standard PCR. In Xenopsylla cheopis fleas collected from Cotonou (Benin), Rickettsia typhi was detected in 1% (2/199), and an uncultured Bartonella sp. was detected in 34.7% (69/199). In the Lushoto district (United Republic of Tanzania), R. typhi DNA was detected in 10% (2/20) of Xenopsylla brasiliensis, and Rickettsia felis was detected in 65% (13/20) of Ctenocephalides felis strongylus, 71.4% (5/7) of Ctenocephalides canis and 25% (5/20) of Ctenophthalmus calceatus calceatus. In the Democratic Republic of the Congo, R. felis was detected in 56.5% (13/23) of Ct. f. felis from Kinshasa, in 26.3% (10/38) of Ct. f. felis and 9% (1/11) of Leptopsylla aethiopica aethiopica from Ituri district and in 19.2% (5/26) of Ct. f. strongylus and 4.7% (1/21) of Echidnophaga gallinacea. Bartonella sp. was also detected in 36.3% (4/11) of L. a. aethiopica. Finally, in Ituri, Y. pestis DNA was detected in 3.8% (1/26) of Ct. f. strongylus and 10% (3/30) of Pulex irritans from the villages of Wanyale and Zaa. Conclusion Most flea-borne infections are neglected diseases which should be monitored systematically in domestic rural and urban human populations to assess their epidemiological and clinical relevance. Finally, the presence of Y. pestis DNA in fleas captured in households was unexpected and raises a series of questions regarding the role of free fleas in the transmission of plague in rural Africa, especially in remote areas where the flea density in houses is high. PMID:25299702

Leulmi, Hamza; Socolovschi, Cristina; Laudisoit, Anne; Houemenou, Gualbert; Davoust, Bernard; Bitam, Idir; Raoult, Didier; Parola, Philippe

2014-01-01

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Biopsia Ecoguiada De La Axila Y Disección Axilar Mínima Ganglionar, Como Alternativas Para La Identificación De Metástasis En El Ganglio Centinela En Cáncer De Mama  

Scientific Electronic Library Online (English)

Full Text Available SciELO Venezuela | Language: Spanish Abstract in spanish OBJETIVOS: Investigar la presencia de metástasis de los ganglios centinela y como alternativa a los métodos tradicionales (Radiofármaco Tc 99 y colorante), MÉTODOS: En 128 pacientes con diagnóstico de cáncer de mama se evaluaron la presencia de ganglios linfáticos axilares. Se utilizó una biopsia ec [...] oguiada de los ganglios de la axila, cuando son vistos al ultrasonido, una disección axilar mínima ganglionar. Se compararon los resultados con la disección axilar estándar. El diagnóstico histológico del tumor primario se realizó por biopsia ecoguiada o por estereotaxia. RESULTADOS: La biopsia ecoguiada de la axila identificó 22 % de metástasis en ganglios centinelas. La disección axilar mínima ganglionar se practicó en el 55 % de las 66 pacientes operadas, fue capaz de identificar ganglios centinelas con metástasis en el 37 %. El ultrasonido es capaz de identificar un 36 % de lesiones subclínicas. La muestra se realizó fundamentalmente en tumores T1 y T2, y cuando la biopsia ecoguiada de la axila identifica metástasis axilares, identifica pacientes de riesgo elevado, independientemente del tamaño de su tumor, que pudieran beneficiarse de un tratamiento de inducción. CONCLUSIONES: El cirujano mastólogo debería utilizar el ultrasonido mamario para la evaluación integral de las pacientes. La biopsia ecoguiada de la axila y la disección axilar mínima ganglionar pudieran ser métodos para la determinación de ganglios centinela, en forma más accesible a hospitales públicos, evitando la morbilidad de la disección axilar completa en tumores pequeños con ganglios negativos. Abstract in english OBJECTIVES: o investigate the presence of metastasis of the sentinel node and like alternative to the traditional methods (Radio pharmacy Tc 99 and colorant), METHODS: In 128 patients with diagnosis of breast cancer, we evaluated the presence of axillary lymphatic nodes. We used an ecoguide biopsy o [...] f the axillary lymphatic nodes, when they are seen by ultrasound, a minimal axillary nodes dissection. The results were compared with the standard axillary dissection. The histological diagnose of the primary tumor was made by ecoguide biopsy or estereotaxia biopsy. RESULTS: The axillary lymphatic nodes ecoguide biopsy identified 22 % of metastasic sentinel nodes. The minimal axillary nodes dissection was practiced in 55 % of the 66 operated patients, and was able to identify the metastasic sentinel nodes in 37 % patients. The ultrasound was able to identify subclinical lesions in 36 % of the cases. The biopsy samples was performed fundamentally in T1 and T2 tumors; when the ecoguide biopsy of axillary nodes identifies axillary metastasis, it identifies high risk patients, independently as large as its tumor, than they could benefit from an induction treatment. CONCLUSIONS: The breast surgeon would have to use the breast ultrasound for an integral evaluation of the patients. The ecoguide biopsy of the axillary lymphatic nodes and the minimal axillary nodes dissection could be methods for the determination of sentinel nodes, in more accessible way to public hospitals, avoiding the morbidity of a complete axillary dissection of the in small tumors.

JORGE, URIBE; MARÍA EUGENIA, MÁRQUEZ; NAYSA, BOSCÁsN; FRANCISCO, MENOLASCINO; JORGE LUIS, URIBE A.

2006-06-01

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Isolation of a Spotted Fever Group Rickettsia, Rickettsia peacockii, in a Rocky Mountain Wood Tick, Dermacentor andersoni, Cell Line  

OpenAIRE

An embryonic cell line (DAE100) of the Rocky Mountain wood tick, Dermacentor andersoni, was observed by microscopy to be chronically infected with a rickettsialike organism. The organism was identified as a spotted fever group (SFG) rickettsia by PCR amplification and sequencing of portions of the 16S rRNA, citrate synthase, Rickettsia genus-specific 17-kDa antigen, and SFG-specific 190-kDa outer membrane protein A (rOmpA) genes. Sequence analysis of a partial rompA gene PCR fragment and indi...

Simser, Jason A.; Palmer, Ann T.; Munderloh, Ulrike G.; Kurtti, Timothy J.

2001-01-01

73

Serological identification of Rickettsia spp from the spotted fever group in capybaras in the region of Campinas - SP - Brazil / Identificação sorológica de Rickettsia spp do grupo da febre maculosa em capivaras na região de Campinas, SP, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Doenças transmitidas por carrapatos vêm sendo um importante problema de saúde pública no mundo. A Febre Maculosa Brasileira (FMB) representa um sério risco epidemiológico devido às altas taxas de letalidade apresentadas. As capivaras são freqüentemente incriminadas como possíveis reservatórios no ci [...] clo de transmissão da FMB. Nas últimas décadas o número desses animais cresceu intensamente e eles invadiram os espaços humanos. As capivaras intensificam o contato entre carrapatos e seres humanos na medida em que se apresentam muito infestadas por estes parasitos. O objetivo deste estudo é contribuir para o conhecimento do possível papel desempenhado por este roedor na epidemiologia da FMB em algumas áreas da região de Campinas, SP. Foi estudada a infecção das capivaras por rickettsias do grupo da FMB, por meio da análise das freqüências de anticorpos contra este grupo, nestes animais, e dados da vigilância epidemiológica de casos humanos. A freqüência desses anticorpos variou amplamente entre as localidades, entretanto, só foram encontrados soros com anticorpos com titulagem =64 naquelas onde havia notificação de casos humanos. Estes achados sugerem que a capivara poderá ser um animal sentinela. No entanto, devido à ocorrência de reação cruzada entre os microorganismos do grupo de FM estes resultados devem ser interpretados com cautela e são necessários métodos capazes de distinguir rickettsias patogênicas. Abstract in english Diseases transmitted by ticks have been an important health problem all over the world. Brazilian Spotted Fever (BSF) stands for a serious epidemiological concern due to the high mortality rates pointed out. Capybaras are commonly incriminated as possible reservoirs in the BSF transmission cycle. In [...] the last decades the numbers of these animals raised sharply and they have invaded human areas. They intensify the contact between ticks and humans beings. This study aim is to contribute to the possible role performed for this rodent in the BSF epidemiology in some areas located in Campinas region, São Paulo. Cabybaras infected by rickettsiae of BSF group were studied through the analysis of the frequencies of BSF-group rickettisae antibodies titer = 64 by indirect immunofluorescence test (IFA), and data from human cases epidemiological surveillance. The serum frequency positiveness varied greatly according to areas where animals were captured. However it was found serum positiviness only in the areas where human cases of BSF were reported. These findings suggest the capybara may be seen as sentinel animal. Due to presence of serological cross reactivity between microorganisms belonging to SF group, the results must be interpreted carefully and additional methods to distinguish pathogenic rickettsiae are required in our country.

Celso Eduardo de, Souza; Savina Silvana Lacerra de, Souza; Virgília Luna Castor, Lima; Simone Berger, Calic; Maria Cecilia Gibrail Oliveira, Camargo; Elisa San Martin Mouriz, Savani; Sandra Regina Nicoletti, D' Auria; Arício Xavier, Linhares; Natalino Hajime, Yoshinari.

1694-16-01

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Efecto de la hipertensión ocular en la población de células ganglionares de la retina de rata y ratón  

OpenAIRE

En esta tesis estudiamos la población total de las células ganglionares de la retina (CGR) en rata y ratón, y desarrollamos un modelo experimental de hipertensión ocular mediante fotocoagulación láser. La población de CGR proyecta masivamente a los colículos superiores. Se observa una estría visual en la retina dorsal donde se encuentra las densidades más altas de CGR. La pequeña población de CGR ipsilateral se distribuye mayoritariamente en la periferia de la retina temporal. El ...

Salinas Navarro, Manuel A?ngel

2011-01-01

75

Regeneración axonal de las células ganglionares de la retina de ratones albinos en injerto autólogo de nervio periférico  

OpenAIRE

La población de células ganglionares de la retina (CGR) de roedores se ha utilizado frecuentemente para investigar las respuestas degenerativas y regenerativas de las neuronas del sistema nervioso central (SNC) en diferentes modelos experimentales de neuropatías, así como para ensayar las propiedades neuroprotectoras de distintas sustancias. En este trabajo hemos analizado cuantitativa y topográficamente la población total de CGRs en ratones adultos albinos que sobreviven a las cuatro s...

Zorrilla Matilla, Laura Patricia

2013-01-01

76

Factores predictivos de metástasis Ganglionares axilares, en Cáncer de mama menor de 2 centímetros  

Scientific Electronic Library Online (English)

Full Text Available SciELO Venezuela | Language: Spanish Abstract in spanish OBJETIVOS: Demostrar que existen factores clínicopatológicos para predecir metástasis ganglionares axilares en tumores de mama de más o menos 20 mm, de diámetro y también que la disección axilar es un procedimiento innecesario en la mayoría de estas pacientes, que puede omitirse con seguridad en aqu [...] ellas pacientes con factores pronósticos favorables. MÉTODOS: Se realiza un estudio retrospectivo, revisándose los reportes macroscópicos, microscópicos, e inmunohistoquímica en los bloques celulares de pacientes con carcinoma mamario de tamaño hasta 20 mm tratadas en el Instituto Oncológico "Dr. Luis Razzeti", entre enero 2000 y diciembre de 2003, determinándose factores que influyen en la aparición de metástasis axilares, realizándose análisis de uni y multivariables. RESULTADOS: El trabajo consistió en una población de 121 pacientes, con una edad media de 57 años, 50 (41,32 %) que presentaron metástasis ganglionar axilar; los factores que se relacionaron con ganglios axilares positivos en el análisis de univariables fueron: grado histológico y nuclear, invasión linfovascular, índice mitótico elevado y tumores aneuploides (P Abstract in english OBJECTIVES: To identify and study that existing pathologically factors and clinical predict nodal metastases axillaries in mammary tumors with diameter size more and minor of 20 mm, so demonstrated in the axillaries dissection is an unnecessary procedure in most of these patients, and can omit with [...] surely in patients with favorable predictive factors. METHODS: We realize a retrospective study, review the macroscopic and microscopic reports, and the inmunohistochemestry in the cellular blocks of patients with breast carcinoma with size until 20 mm treated in the Oncology Institute "Dr. Luis Razetti", between January and December of 2000 - 2003, determining factors that influence in the appearance of lymph nodes axillaries positives, making unvaried and multivariate analysis. RESULTS: These work consisted in the studied of 121 patients with mean age 57 years, 50 (41, 32 %) presented axillaries disease nodes metastases; the factors that were related to positive lymph nodes in the unavailable analysis were: histological and nuclear grade, linfovascular invasion, mitotic index high, aneuploid tumors (P

Alí Josué, Godoy Briceño; Luís, Betancourt; David, Parada; Sergio Osorio, Morales.

2007-12-01

77

Vigilancia Epidemiológica de Rickettsia‚ Ehrlichia y Anaplasma en Fauna Silvestre ex situ e in situ de algunas Regiones de Colombia -resumen-  

Directory of Open Access Journals (Sweden)

Full Text Available Las enfermedades causadas por bacterias del orden de los Rickettsiales son trasmitidas por ectoparásitos originados en vida silvestre‚ sin embargo‚ en Colombia no existe una línea base del conocimiento en este sentido. Estos microorganismos intracelulares son causantes de enfermedades transmisibles en pacientes humanos; convirtiéndolas en zoonosis de tipo emergente. Este estudio ha permitido determinar la presencia de microorganismos del orden de los Rickettsiales (Ehrlichia spp.‚ Anaplasma spp.‚ y Rickettsia spp. en ejemplares silvestres de centros de conservación ex situ (Zoológicos‚ CAV y CAVR y en algunas regiones en condiciones in situ del país. De igual manera se identifica la infección de estos microorganismos en ectoparásitos vectores relacionados. Por medio de la técnica PCRrt‚ se detectó la presencia de Rickettsia spp.‚ Anaplasma spp.‚ y Ehrlichia spp.‚ en suero obtenido de mamíferos‚ y garrapatas colectadas de reptiles y mamíferos del Centro de Atención y Valoración de Fauna silvestre de la Corporación Autónoma Regional de los Valles del Sinú y San Jorge CAV-CVS en Montería (Córdoba‚ en osos hormigueros (Myrmecophaga tridactyla en Pore (Casanare‚ y en garrapatas obtenidas en ejemplares de titi gris (Saguinus leucopus y su hábitat en la vereda La Parroquia en Mariquita (Tolima. En Montería‚ los resultados obtenidos en mamíferos demostraron la ausencia de Rickettsia spp. en 72 muestras de suero‚ sin embargo se detectó un pool de garrapatas de la especie Amblyomma ovale positivo a este microorganismo‚ siendo el primer reporte en Colombia de ectoparásitos de Puma concolor positivos a Rickettsia spp.‚ mientras que en reptiles se detectaron 18 pools de garrapatas positivos a Rickettsia spp (90 %. En las garrapatas colectadas de nueve osos palmeros (Myrmecophaga tridactyla de vida libre se detectaron 12 pool de garrapatas positivos a este microorganismo (17‚6 %‚ y en ejemplares de titi gris fue positivo a Rickettsia spp en un pool de garrapatas (50%. Para Anaplasma spp. se detectaron 2 pools de garrapatas positivos a este microorganismo (3‚6% pertenecientes a dos osos palmeros del Casanare. Ningún animal estudiado‚ o ectoparásito ha tenido presencia de Ehrlichia spp. Los resultados obtenidos a la fecha muestran que los microorganismos del orden de los rickettsiales se encuentran circulantes entre garrapatas de animales silvestres en centros de conservación ex situ y en algunas regiones en condiciones in situ. La vigilancia epidemiológica de estos agentes y sus vectores es la clave de una respuesta oportuna y eficiente para prevenir las epidemias causadas por estos patógenos.

Santiago Monsalve-Buriticá

2014-12-01

78

Amiloidose ganglionar mediastinal em paciente com sarcoidose / Mediastinal lymph node amyloidosis in a patient with sarcoidosis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Paciente masculino, 27 anos, com sintomas respiratórios, linfonodomegalia cervical anterior bilateral e hepatomegalia. Os estudos de imagem evidenciaram linfonodomegalia hilar bilateral e infiltrado pulmonar. O paciente foi submetido a biópsias pulmonar e hepática, que evidenciaram presença de granu [...] lomas não caseosos. Também foi submetido à biópsia de linfonodo hilar, que revelou a presença de material amilóide. Os achados clínicos, radiológicos e histopatológicos foram compatíveis com sarcoidose e amiloidose ganglionar. A associação entre sarcoidose e amiloidose é raramente descrita. Abstract in english A 27-year-old male patient presented with respiratory symptoms, bilateral enlargement of the cervical lymph nodes and enlarged liver. In the imaging studies, bilateral enlargement of the hilar nodes was observed, together with pulmonary infiltrate. The patient was submitted to lung and liver biopsie [...] s, which revealed noncaseating granulomas. The clinical, radiological and histopathological findings were consistent with sarcoidosis and lymph node amyloidosis. The combination of sarcoidosis and amyloidosis has rarely been reported.

Lilian, Schade; Eliane Ribeiro, Carmes; João Adriano de, Barros.

2007-04-01

79

Rickettsia lusitaniae sp. nov. isolated from the soft tick Ornithodoros erraticus (Acarina: Argasidae).  

Science.gov (United States)

In this study a novel Rickettsia from the spotted fever group, isolated from Ornithodoros erraticus soft ticks collected from pigpens in the south of Portugal, is described. After initial screening revealed Rickettsia-positive ticks, isolation attempts were then performed. Successful isolates were achieved by shell-vial technique using Vero E6 cells at 28°C. Molecular characterization of the isolate was performed based on analysis of five rickettsial genes gltA, ompA, ompB, sca1 and htr with their subsequent concatenation along with other rickettsial species resulting in a clustering of the new isolate with Rickettsia felis and Rickettsia hoogstraalii. The degree of nucleotide sequence similarity with other rickettsiae fulfills the criteria for classification of our isolate as a novel species. The name Rickettsia lusitaniae sp. nov. (=CEVDI PoTiRo) is proposed for this new species found in O. erraticus. PMID:24513450

Milhano, Natacha; Palma, Mariana; Marcili, Arlei; Núncio, Maria Sofia; de Carvalho, Isabel Lopes; de Sousa, Rita

2014-05-01

80

Rickettsia felis from Cat Fleas: Isolation and Culture in a Tick-Derived Cell Line  

OpenAIRE

Rickettsia felis, the etiologic agent of spotted fever, is maintained in cat fleas by vertical transmission and resembles other tick-borne spotted fever group rickettsiae. In the present study, we utilized an Ixodes scapularis-derived tick cell line, ISE6, to achieve isolation and propagation of R. felis. A cytopathic effect of increased vacuolization was commonly observed in R. felis-infected cells, while lysis of host cells was not evident despite large numbers of rickettsiae. Electron micr...

Pornwiroon, Walairat; Pourciau, Susan S.; Foil, Lane D.; Macaluso, Kevin R.

2006-01-01

81

Rickettsia typhi Possesses Phospholipase A2 Enzymes that Are Involved in Infection of Host Cells  

OpenAIRE

The long-standing proposal that phospholipase A2 (PLA2) enzymes are involved in rickettsial infection of host cells has been given support by the recent characterization of a patatin phospholipase (Pat2) with PLA2 activity from the pathogens Rickettsia prowazekii and R. typhi. However, pat2 is not encoded in all Rickettsia genomes; yet another uncharacterized patatin (Pat1) is indeed ubiquitous. Here, evolutionary analysis of both patatins across 46 Rickettsia genomes revealed 1) pat1 and pat...

Rahman, M. Sayeedur; Gillespie, Joseph J.; Kaur, Simran Jeet; Sears, Khandra T.; Ceraul, Shane M.; Beier-sexton, Magda; Azad, Abdu F.

2013-01-01

82

Rickettsia infection in Amblyomma tonelliae, a tick species from the Amblyomma cajennense complex.  

Science.gov (United States)

The present study was performed to evaluate the Rickettsia infection in Amblyomma tonelliae ticks from Argentina. All ticks were subjected to DNA extraction and tested by a battery of PCRs to amplify fragments of four rickettsial genes, 23S-5S, gltA, ompA and htrA. Two ticks were positive. The Rickettsia detected in one tick represents a new lineage which is named Rickettsia sp. strain El Tunal. This new strain belongs to the canadensis group because it is closely related to Rickettsia monteiroi, Rickettsia canadensis and Candidatus "Rickettsia tarasevichiae". They clustered together on a high supported clade with both gltA and htrA genes. The other positive tick was infected with Candidatus "Rickettsia amblyommii". The results presented in this study constitute the first records of Rickettsia infection in A. tonelliae ticks. However, the medical relevance of these findings should be considered cautiously because the pathogenicity of Rickettsia sp. strain El Tunal and Candidatus "R. amblyommii" remains undetermined. PMID:25544308

Tarragona, Evelina L; Cicuttin, Gabriel L; Mangold, Atilio J; Mastropaolo, Mariano; Nazarena De Salvo, M; Nava, Santiago

2015-03-01

83

Serosurveillance of Orientia tsutsugamushi and Rickettsia typhi in Bangladesh.  

Science.gov (United States)

Scrub and murine typhus infections are under-diagnosed causes of febrile illness across the tropics, and it is not known how common they are in Bangladesh. We conducted a prospective seroepidemiologic survey across six major teaching hospitals in Bangladesh by using an IgM enzyme-linked immunosorbent assay. Results indicated recent exposure (287 of 1,209, 23.7% seropositive for Orientia tsutsugamushi and 805 of 1,209, 66.6% seropositive for Rickettsia typhi). Seropositive rates were different in each region. However, there was no geographic clustering of seropositive results for both organisms. There was no difference between those from rural or urban areas. Rickettsia typhi seroreactivity was positively correlated with age. Scrub typhus and murine typhus should be considered as possible causes of infection in Bangladesh. PMID:25092819

Maude, Rapeephan R; Maude, Richard J; Ghose, Aniruddha; Amin, M Robed; Islam, M Belalul; Ali, Mohammad; Bari, M Shafiqul; Majumder, M Ishaque; Tanganuchitcharnchai, Ampai; Dondorp, Arjen M; Paris, Daniel H; Bailey, Robin L; Faiz, M Abul; Blacksell, Stuart D; Day, Nicholas P J

2014-09-01

84

Molecular detection of Rickettsia typhi in cats and fleas.  

OpenAIRE

Background: Rickettsia typhi is the etiological agent of murine typhus (MT), a disease transmitted by two cycles: rat-flea-rat, and peridomestic cycle. Murine typhus is often misdiagnosed and underreported. A correct diagnosis is important because MT can cause severe illness and death. Our previous seroprevalence results pointed to presence of human R. typhi infection in our region; however, no clinical case has been reported. Although cats have been related to MT, no naturally infected cat h...

Nogueras, Maria Mercedes

2013-01-01

85

Horizontal transmission of Rickettsia felis between cat fleas, Ctenocephalides felis  

OpenAIRE

Rickettsia felis is a rickettsial pathogen primarily associated with the cat flea, Ctenocephalides felis. Although laboratory studies have confirmed that R. felis is maintained by transstadial and transovarial transmission in C. felis, distinct mechanisms of horizontal transmission of R. felis among cat fleas are undefined. Based on the inefficient vertical transmission of R. felis by cat fleas and the detection of R. felis in a variety of haematophagous arthropods, we hypothesize that R. fel...

Hirunkanokpun, Supanee; Thepparit, Chutima; Foil, Lane D.; Macaluso, Kevin R.

2011-01-01

86

Artificial infection of the bed bug with Rickettsia parkeri.  

Science.gov (United States)

Although a variety of disease agents have been reported from bed bugs, the mechanical and biological disease transmission potential of bed bugs remains unelucidated. In this study we assayed survivability of the mildly pathogenic spotted fever group rickettsia, Rickettsia parkeri, in bed bugs after feeding on R. parkeri-infected chicken blood. Two groups of 15 adult bed bugs each were fed on infected or noninfected blood, and two groups of fourth-instar bed bugs also were fed on either infected or noninfected blood. One group of 15 adult bed bugs received no bloodmeal and was included as an additional control. Two weeks postfeeding, two pools of five live bed bugs from each group were surface sterilized, macerated, and placed in Vero cell cultures in an attempt to grow live organism. The remaining five individual bed bugs from each group were dissected, their salivary glands were removed for immunofluorescence assay (IFA) staining, and the remaining body parts were processed for polymerase chain reaction (PCR) analysis. Results indicated that no immature (now molted to fifth instar) bed bugs were positive for R. parkeri by IFA or PCR, indicating that organisms did not survive the molting process. After 4 wk of cell culture, no organisms were seen in cultures from any of the treatment or control groups, nor were any cultures PCR positive. However, two of the adult bed bugs were IFA positive for rickettsia-like organisms, and these two specimens were also PCR positive using R. parkeri-specific primers. These IFA and PCR results indicate that remnants of Rickettsia parkeri (possibly whole organisms) survived in the bugs for 2 wk, but the viability of the organisms in these two specimens could not be determined. PMID:22897053

Goddard, Jerome; Varela-Stokes, Andrea; Smith, Whitney; Edwards, Kristine T

2012-07-01

87

Isolation of Rickettsia parkeri and identification of a novel spotted fever group Rickettsia sp. from Gulf Coast ticks (Amblyomma maculatum) in the United States.  

Science.gov (United States)

Until recently, Amblyomma maculatum (the Gulf Coast tick) had garnered little attention compared to other species of human-biting ticks in the United States. A. maculatum is now recognized as the principal vector of Rickettsia parkeri, a pathogenic spotted fever group rickettsia (SFGR) that causes an eschar-associated illness in humans that resembles Rocky Mountain spotted fever. A novel SFGR, distinct from other recognized Rickettsia spp., has also been detected recently in A. maculatum specimens collected in several regions of the southeastern United States. In this study, 198 questing adult Gulf Coast ticks were collected at 4 locations in Florida and Mississippi; 28% of these ticks were infected with R. parkeri, and 2% of these were infected with a novel SFGR. Seventeen isolates of R. parkeri from individual specimens of A. maculatum were cultivated in Vero E6 cells; however, all attempts to isolate the novel SFGR were unsuccessful. Partial genetic characterization of the novel SFGR revealed identity with several recently described, incompletely characterized, and noncultivated SFGR, including "Candidatus Rickettsia andeanae" and Rickettsia sp. Argentina detected in several species of Neotropical ticks from Argentina and Peru. These findings suggest that each of these "novel" rickettsiae represent the same species. This study considerably expanded the number of low-passage, A. maculatum-derived isolates of R. parkeri and characterized a second, sympatric Rickettsia sp. found in Gulf Coast ticks. PMID:20208020

Paddock, Christopher D; Fournier, Pierre-Edouard; Sumner, John W; Goddard, Jerome; Elshenawy, Yasmin; Metcalfe, Maureen G; Loftis, Amanda D; Varela-Stokes, Andrea

2010-05-01

88

Spotted fever group rickettsiae in ixodid ticks in Oromia, Ethiopia.  

Science.gov (United States)

In Ethiopia, information on the transmission of human zoonotic pathogens through ixodid ticks remains scarce. To address the occurrence and molecular identity of spotted fever group rickettsiae using molecular tools, a total of 767 ixodid ticks belonging to thirteen different species were collected from domestic animals from September 2011 to March 2014. Rickettsia africae DNA was detected in 30.2% (16/53) Amblyommma variegatum, 28.6% (12/42) Am. gemma, 0.8% (1/119) Am. cohaerens, 18.2% (4/22) Amblyomma larvae, 6.7% (2/60) Amblyomma nymphs, 0.7% (1/139) Rhipicephalus (Boophilus) decoloratus and 25% (1/4) nymphs of Rh. (Bo.) decoloratus. A markedly low prevalence of R. africae was recorded in both Am. cohaerens and Rh. (Bo.) decoloratus (pEthiopia is presented herein. Altogether, these results suggest that the transmission of spotted fever group rickettsiae through ixodid ticks is a potential risk for human health in different parts of Ethiopia. Clinicians in this country should consider these pathogens as a potential cause of febrile illness in patients. PMID:25262832

Kumsa, Bersissa; Socolovschi, Cristina; Raoult, Didier; Parola, Philippe

2015-02-01

89

Genotyping, evolution and epidemiological findings of Rickettsia species.  

Science.gov (United States)

Rickettsiae are obligate intracellular bacteria that can cause mild to life-threatening diseases, including epidemic typhus, one of the oldest pernicious diseases of mankind. Clinical awareness of rickettsial diseases and molecular diagnosis have shown that rickettsioses should be viewed as new emerging and reemerging diseases. Rickettsia has been shown to be a large genus with a worldwide distribution, a very diverse host range, including hosts that have no relationship with vertebrate. Genomic studies have demonstrated genome reduction due to gene loss associated with increased pathogenicity and horizontal DNA acquisition according to a sympatric mode of evolution in hosts that contain several organisms. This article presents a review of genotyping techniques and examines the principle of genotype determination in terms of taxonomic strategies and detection methods. This article summarizes the epidemiological and pathological features of Rickettsia and discusses the genomic findings that help the understanding of the evolution of pathogenicity including the deleterious mutations of repair systems and the toxin-antitoxin systems. PMID:24662440

Merhej, Vicky; Angelakis, Emmanouil; Socolovschi, Cristina; Raoult, Didier

2014-07-01

90

Rickettsia felis infection in cat fleas Ctenocephalides felis felis  

Directory of Open Access Journals (Sweden)

Full Text Available The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females tested by polymerase chain reaction (PCR were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5% out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory, 6 (40.0% reacted positively to R. felis by IFA. Reactive feline and human sera showed low endpoint titers against R. felis, varying from 64 to 256. With the exception of one human serum, all R. felis-reactive sera were also reactive to Rickettsia rickettsii and/or Rickettsia parkeri antigens at similar titers to R. felis. The single human serum that was reactive solely to R. felis had an endpoint titer of 256, indicating that this person was infected by R. felis.

Mauricio C. Horta

2010-10-01

91

Rickettsia felis infection in cat fleas Ctenocephalides felis felis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR [...] products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA) showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5%) out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory, 6 (40.0%) reacted positively to R. felis by IFA. Reactive feline and human sera showed low endpoint titers against R. felis, varying from 64 to 256. With the exception of one human serum, all R. felis-reactive sera were also reactive to Rickettsia rickettsii and/or Rickettsia parkeri antigens at similar titers to R. felis. The single human serum that was reactive solely to R. felis had an endpoint titer of 256, indicating that this person was infected by R. felis.

Mauricio C., Horta; Fabio B., Scott; Thaís R., Correia; Julio I., Fernandes; Leonardo J., Richtzenhain; Marcelo B., Labruna.

2010-10-01

92

Comparación de la Tomografía Axial Computarizada con el estudio anatomopatológico en el estadiaje ganglionar del cáncer de pulmón  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish PROPÓSITO: Evaluar la Sensibilidad, Especificidad, Valor predictivo positivo y Valor Predictivo Negativo de la Tomografia Axial Computarizada (TAC) en el estadiaje ganglionar del cáncer de pulmón. MATERIAL Y MÉTODOS: Se han analizado 30 pacientes diagnosticados de carcinoma pulmonar entre Mayo de 20 [...] 03 y Mayo de 2004. A todos los pacientes se les realizó una TAC torácica, valorando la afectación ganglionar mediastínica. A su vez, se les realizó o una mediastinoscopia o una resección pulmonar más linfadenectomía mediastínica, obteniendo así material para el estudio anatomo-patológico para confirmar o no la afectación ganglionar mediastínica y correlacionarla con los hallazgos de la TAC. RESULTADOS: Hemos obtenido una Sensibilidad del 72,2%, una Especificidad del 100%, un valor predictivo positivo del 100% y un valor predictivo negativo del 70,6% para la TAC, utilizando como "gold standar" el estudio anatomopatológico. CONCLUSIONES: La TAC torácica se considera una prueba de imagen de rutina en el diagnóstico del cáncer de pulmón; pero en muchos casos no es capaz de estadiar correctamente la afectación ganglionar mediastínica. Es en estos casos, donde es necesario realizar pruebas invasivas como la mediastinoscopia. Actualmente, la aparición de la PET permite estadiar mejor el tumor, ofreciendo mejor tratamiento a cada paciente, y en determinados casos evitar técnicas diagnósticas invasivas. Abstract in english PURPOSE: To analise the sensitivity, specificity, and positive and negative predictive values of the computerized axial tomography (CT) in nodal stages of lung carcinoma. MATERIAL AND METHODS: Thirty patients suffering from lung carcinoma were analysed between May 2003 and May 2004. CT screening of [...] the thorax was made to all the patients. Mediastinoscopy or lung resection samples plus systematic node dissection were performed, and the efficiency of CT analysed by comparing the obtained data with the histopathology results of nodal affection shown by mediastinoscopy and lymph node dissection. RESULTS: The sensitivity, specificity, and positive and negative predictive value of the computerized axial tomography (CT) for nodal staging involved in lung carcinoma were 72.2%, 100%, 100% and 70,6% respectively. CONCLUSIONS: CT of the thorax is considered a usual imaging technique in lung carcinoma diagnosis; but in some cases it does not focalise correctly the nodal staging involved in lung carcinoma. In theses cases, it is necessarry to perform invasive techniques such as mediastinoscopy. At present, positron emission tomography (PET) is the technique that better permits to focalise the tumor offering the best data for the therapy of each patient, and avoiding invasive diagnosis techniques.

M., López Mata; J., Valencia Julve; N., Bascón Santaló; C., Velilla Millán; R., Escó Barón; M., García Álvarez.

2005-07-01

93

Rickettsia symbionts cause parthenogenetic reproduction in the parasitoid wasp Pnigalio soemius (Hymenoptera: Eulophidae).  

Science.gov (United States)

Bacteria in the genus Rickettsia are intracellular symbionts of disparate groups of organisms. Some Rickettsia strains infect vertebrate animals and plants, where they cause diseases, but most strains are vertically inherited symbionts of invertebrates. In insects Rickettsia symbionts are known to have diverse effects on hosts ranging from influencing host fitness to manipulating reproduction. Here we provide evidence that a Rickettsia symbiont causes thelytokous parthenogenesis (in which mothers produce only daughters from unfertilized eggs) in a parasitoid wasp, Pnigalio soemius (Hymenoptera: Eulophidae). Feeding antibiotics to thelytokous female wasps resulted in production of progeny that were almost all males. Cloning and sequencing of a fragment of the 16S rRNA gene amplified with universal primers, diagnostic PCR screening of symbiont lineages associated with manipulation of reproduction, and fluorescence in situ hybridization (FISH) revealed that Rickettsia is always associated with thelytokous P. soemius and that no other bacteria that manipulate reproduction are present. Molecular analyses and FISH showed that Rickettsia is distributed in the reproductive tissues and is transovarially transmitted from mothers to offspring. Comparison of antibiotic-treated females and untreated females showed that infection had no cost. Phylogenetic analyses of 16S rRNA and gltA gene sequences placed the symbiont of P. soemius in the bellii group and indicated that there have been two separate origins of the parthenogenesis-inducing phenotype in the genus Rickettsia. A possible route for evolution of induction of parthenogenesis in the two distantly related Rickettsia lineages is discussed. PMID:20173065

Giorgini, M; Bernardo, U; Monti, M M; Nappo, A G; Gebiola, M

2010-04-01

94

Gamma-irradiated scrub typhus immunogens: analysis for residual replicating rickettsiae  

Energy Technology Data Exchange (ETDEWEB)

Scrub thyphus immunogens that received inadequate gamma radiation contained residual, viable rickettsiae. The presence of these organisms in the host was masked by the rapid immune response elicited by the large number of inactivated rickettsiae. Transfer of homogenized spleen cells from immunized mice to normal syngeneic recipients provided a sensitive technique for the detection of these viable, replicating organisms.

Eisenberg, G.H. Jr.; Osterman, J.V.; Stephenson, E.H.

1980-04-01

95

A Typhus Group-Specific Protease Defies Reductive Evolution in Rickettsiae ? † ‡  

OpenAIRE

Phylogenomics reveals extreme gene loss in typhus group (TG) rickettsiae relative to the levels for other rickettsial lineages. We report here a curious protease-encoding gene (ppcE) that is conserved only in TG rickettsiae. As a possible determinant of host pathogenicity, ppcE warrants consideration in the development of therapeutics against epidemic and murine typhus.

Ammerman, Nicole C.; Gillespie, Joseph J.; Neuwald, Andrew F.; Sobral, Bruno W.; Azad, Abdu F.

2009-01-01

96

Gamma-irradiated scrub typhus immunogens: analysis for residual replicating rickettsiae  

International Nuclear Information System (INIS)

Scrub thyphus immunogens that received inadequate gamma radiation contained residual, viable rickettsiae. The presence of these organisms in the host was masked by the rapid immune response elicited by the large number of inactivated rickettsiae. Transfer of homogenized spleen cells from immunized mice to normal syngeneic recipients provided a sensitive technique for the detection of these viable, replicating organisms

97

Genome Sequence of Rickettsia hoogstraalii, a Geographically Widely Distributed Tick-Associated Bacterium  

OpenAIRE

Rickettsia hoogstraalii is a tick-associated member of the spotted fever group rickettsiae that is geographically widely distributed. We report here the draft genome of R. hoogstraalii strain CroaticaT (=DSM 22243 = UTMB 00003), which was isolated from Haemaphysalis sulcata ticks collected in Croatia.

Sentausa, Erwin; El Karkouri, Khalid; Nguyen, Thi-tien; Caputo, Aure?lia; Raoult, Didier; Fournier, Pierre-edouard

2014-01-01

98

Genome Sequence of Rickettsia hoogstraalii, a Geographically Widely Distributed Tick-Associated Bacterium.  

Science.gov (United States)

Rickettsia hoogstraalii is a tick-associated member of the spotted fever group rickettsiae that is geographically widely distributed. We report here the draft genome of R. hoogstraalii strain Croatica(T) (=DSM 22243 = UTMB 00003), which was isolated from Haemaphysalis sulcata ticks collected in Croatia. PMID:25377719

Sentausa, Erwin; El Karkouri, Khalid; Nguyen, Thi-Tien; Caputo, Aurélia; Raoult, Didier; Fournier, Pierre-Edouard

2014-01-01

99

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples  

OpenAIRE

A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Rickettsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

Svendsen, Claus Bo; Boye, Mette; Struve, Carsten; Krogfelt, Karen A.

2009-01-01

100

Comparison of different media for preservation and transport of viable rickettsiae.  

Science.gov (United States)

Rickettsiae tend to have a rapid decrease of viability outside living cells. Therefore, the transport of samples containing viable rickettsiae for culturing in cell culture for diagnostic purposes is challenging. The viability of rickettsiae in different transport media (commercially available transport medium COPAN "UTM-RT transport medium for viruses, chlamydia, mycoplasma, and ureaplasma," minimal essential medium (MEM) with and without 10% foetal calf serum) at various time points at 4 °C and at ambient temperature (22 °C) was compared. Rickettsia honei was used as model organism. After 2 weeks of storage at room temperature, no viable rickettsiae were detectable any more while storage at 4 °C kept rickettsiae viable for up to 4 weeks. The commercially available COPAN medium showed similarly good or slightly better stabilizing effects on rickettsiae compared with MEM + 10% foetal calf serum, pure MEM demonstrated the poorest results. It is important to transport and store media with potentially rickettsiae-containing samples at 4 °C to prevent inactivation. MEM + 10% foetal calf serum can be used if no commercial medium is available with similarly good results. PMID:24265938

Frickmann, Hagen; Dobler, Gerhard

2013-09-01

101

Rickettsia felis Infection in Febrile Patients, Western Kenya, 2007–2010  

OpenAIRE

To determine previous exposure and incidence of rickettsial infections in western Kenya during 2007–2010, we conducted hospital-based surveillance. Antibodies against rickettsiae were detected in 57.4% of previously collected serum samples. In a 2008–2010 prospective study, Rickettsia felis DNA was 2.2× more likely to be detected in febrile than in afebrile persons.

Maina, Alice N.; Knobel, Darryn L.; Jiang, Ju; Halliday, Jo; Feikin, Daniel R.; Cleaveland, Sarah; Ng’ang’a, Zipporah; Junghae, Muthoni; Breiman, Robert F.; Richards, Allen L.; Njenga, M. Kariuki

2012-01-01

102

Rickettsia felis infection in a common household insect pest, Liposcelis bostrychophila (Psocoptera: Liposcelidae).  

Science.gov (United States)

Many species of Rickettsia are well-known mammalian pathogens transmitted by blood-feeding arthropods. However, molecular surveys are continually uncovering novel Rickettsia species, often in unexpected hosts, including many arthropods that do not feed on blood. This study reports a systematic molecular characterization of a Rickettsia infecting the psocid Liposcelis bostrychophila (Psocoptera: Liposcelidae), a common and cosmopolitan household pest. Surprisingly, the psocid Rickettsia is shown to be Rickettsia felis, a human pathogen transmitted by fleas that causes serious morbidity and occasional mortality. The plasmid from the psocid R. felis was sequenced and was found to be virtually identical to the one in R. felis from fleas. As Liposcelis insects are often intimately associated with humans and other vertebrates, it is speculated that they acquired R. felis from fleas. Whether the R. felis in psocids causes disease in vertebrates is not known and warrants further study. PMID:20139311

Behar, Adi; McCormick, Laurie J; Perlman, Steve J

2010-04-01

103

Rickettsiae and giant lysosomes in the testes of Temnocephala novaezealandiae (Platyhelminthes: Temnocephaloidea).  

Science.gov (United States)

Rickettsiae are concentrated in the testes of Temnocephala novaezealandiae, where they occupy the cytoplasm of spermatogenetic stages and the testis epithelia. They have gram negative ultrastructural characteristics and are surrounded by clear zones which are not membrane-limited; indented mitochondria are associated with the clear zones. Propagation occurs by simple division. Extensions of the testis epithelium envelop cloned groups of spermatocytes, spermatids and spermatozoa. Heterophagosomes isolating unmodified rickettsiae, and giant lysosomes enclosing bacteria in advanced stages of degradation, are contained within the epithelial processes. Rickettsiae and large lysosomes were found also in testes of Troglocaridicola mrazeki, a scutariellid. Peririckettsial lucid zones are interpreted as areas of histopathy where host cytoplasm has been catabolized by bacterial enzymes. The rickettsiae evidently are true parasites, although they have no apparent harmful effect on the general condition of their hosts. Digestion of bacteria in lysosomes may provide nutrients for spermatogenetic stages. Transmission of rickettsiae to juvenile temnocephalids does not occur via the spermatozoa. PMID:1913590

Williams, J B

1991-07-01

104

Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil / Identificação sorológica de Rickettsia sp. em equinos e cães de área não endêmica no Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese A Febre Maculosa Brasileira (FMB) é uma riquetsiose letal para humanos, causada pela bactéria Rickettsia rickettsii, e é endêmica em algumas regiões brasileiras. Equinos e cães podem participar do ciclo da doença e podem também servir como sentinelas em estudos epidemiológicos. O primeiro caso human [...] o relatado no Estado do Paraná ocorreu em 2005. O presente estudo foi realizado no município de Almirante Tamandaré, região onde não há relatos de casos de FMB. Foram coletadas amostras de sangue de 71 cavalos e 20 cães em nove propriedades rurais na região. Carrapatos também foram colhidos dos animais. Todos os proprietários responderam a um questionário sobre o manejo sanitário dos animais e o conhecimento a respeito da FMB. As amostras de soro foram processadas pela técnica de Reação de Imunofluorescência Indireta (RIFI), utilizando-se os antígenos de R. rickettsii e R. parkeri. Os carrapatos foram analisados por PCR para Rickettsia sp. e todos foram negativos. Seis cavalos (8,45%) e 4 cães (20%) foram identificados como soropositivos. Todos os proprietários desconheciam a relação de carrapatos com a FMB. Embora considerada uma área não endêmica, Almirante Tamandaré é um ambiente vulnerável à FMB e um controle eficiente de carrapatos deve ser implementado. Abstract in english Brazilian Spotted Fever (BSF) is a lethal rickettsiosis in humans caused by the bacteria Rickettsia rickettsii, and is endemic in some areas of Brazil. Horses and dogs are part of the disease's life cycle and they may also serve as sentinel animals in epidemiological studies. The first human BSF cas [...] e in the State of Paraná was reported in 2005. The present study was conducted in the municipality of Almirante Tamandaré, where no previous case of BSF was reported. Serum samples were collected from 71 horses and 20 dogs from nine properties in the area. Ticks were also collected from these animals. All farmers completed a questionnaire about their knowledge of BSF and animal health management. Serum samples were analyzed by indirect immunofluorescent-antibody assay (IFA) using R. rickettsii and R. parkeri as antigens. Ticks were analyzed by PCR for Rickettsia sp., and all of them were PCR-negative. Six horses (8.45%) and 4 dogs (20%) were identified as seropositive. Farmers were not aware of the correlation between the presence of ticks and risk of BSF. Although a non-endemic area, Almirante Tamandaré is a vulnerable environment for BSF and effective tick control measures are required.

Fernanda Gonçalves, Batista; Daniella Matos da, Silva; Kerriel Thandile, Green; Louise Boulsfield de Lorenzi, Tezza; Sâmara Pereira de, Vasconcelos; Suelen Graziele Soares de, Carvalho; Iara, Silveira; Jonas, Moraes-Filho; Marcelo Bahia, Labruna; Fernanda Silva, Fortes; Marcelo Beltrão, Molento.

2010-12-01

105

Changes in immunoferritin labeling of Rickettsia tsutsugamushi after serial cultivation in 60Co-irradiated BHK cells  

International Nuclear Information System (INIS)

The immunolabeling characteritics of Rickettsia tsutsugamushi (Gilliam strain) were examined by using a purified immunoglobulin G fraction of antibody to R. tsutsugamushi raised in rabbits. When rickettsiae in BHK-21 cells infected from yolk sac seed material were immunoferritin labeled, the binding of ferritin was found to be dense and uniform on the outer surface of the rickettsiae in disrupted host cells. Immunolabeling of purified suspensions of extracellular rickettsiae resulted in the uniform ferritin labeling of the microorganism. The immunoferritin labeling of R. tsutsugamushi during successive serial passages in BHK-21 cells revealed decreased labeling with each passage, and by the 10th passage there was no detectable labeling. However, these rickettsiae inoculated back into yolk sacs regained their immunoferritin labeling. Antibody against rickettsiae cultivated in BHK-21 cells continued labeling rickettsiae even after 9 serial passages in BHK-21 cells

106

Detection of Rickettsia tsutsugamushi in Experimentally infected mice by PCR.  

OpenAIRE

We developed a rapid procedure for the detection of Rickettsia tsutsugamushi DNA by the PCR technique. The primer pair used for the PCR was designed from the DNA sequence of the gene encoding a 120-kDa antigen, which was proven to be group specific by immunoblot analysis with mouse hyperimmune sera against various rickettsial strains. This PCR method was able to detect up to 10 ag of plasmid DNA (pKT12). Specific PCR products were obtained with DNAs from R. tsutsugamushi Kato, Karp, Gilliam, ...

Kee, S. H.; Choi, I. H.; Choi, M. S.; Kim, I. S.; Chang, W. H.

1994-01-01

107

Actualización en metástasis ganglionar de carcinoma escamoso de cabeza y cuello: Disección ganglionar, ganglio centinela y técnicas de biología molecular / Update in lymph node metastasis from head and neck squamous cell carcinoma: Lymph node dissection, sentinel lymph node and molecular biology techniques  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish El carcinoma escamoso es la principal neoplasia maligna de cabeza y cuello en los adultos. Esta neoplasia se origina en la mucosa del tracto aerodigestivo superior. Se discute su extensión en superficie y las metástasis a ganglios linfáticos cervicales. El compromiso ganglionar es el principal facto [...] r pronóstico independiente del carcinoma escamoso de cabeza y cuello, pues la presencia de adenopatías metastásicas reduce la sobrevida casi en 50%. La siguiente revisión se centra en tres temas relacionados con las metástasis ganglionares en carcinoma escamoso de cabeza y cuello (CECC): la clasificación de niveles ganglionares y de la disección ganglionar cervical, la técnica del ganglio centinela en el CECC y las técnicas de biología molecular para el diagnóstico del compromiso tumoral ganglionar Abstract in english Squamous cell carcinoma is the main head and neck malignant cancer in adults. This cancer originates from the upper aero digestive tract mucosa. Its surface extension and cervical lymph node metastases are discussed. Lymph node involvement is the main independent prognostic factor in head and neck s [...] quamous cell carcinoma, given that the presence of metastatic nodes reduce survival by approximately 50%. The present review focus on three topics related to lymph nodes metastasis of head and neck squamous cell carcinomas (CECC): Classification of lymph node levels and cervical node dissection, the sentinel lymph node technique in CECC, and the use of molecular biology techniques for diagnosing lymph node involvement

Pablo, Ortega R.

2008-04-01

108

On the use of Legionella/Rickettsia chimeras to investigate the structure and regulation of Rickettsia effector RalF.  

Science.gov (United States)

A convenient strategy to interrogate the biology of regulatory proteins is to replace individual domains by an equivalent domain from a related protein of the same species or from an ortholog of another species. It is generally assumed that the overall properties of the native protein are retained in the chimera, and that functional differences reflect only the specific determinants contained in the swapped domains. Here we used this strategy to circumvent the difficulty in obtaining crystals of Rickettsia prowazekii RalF, a bacterial protein that functions as a guanine nucleotide exchange factor for eukaryotic Arf GTPases. A RalF homolog is encoded by Legionella pneumophila, in which a C-terminal capping domain auto-inhibits the catalytic Sec7 domain and localizes the protein to the Legionella-containing vacuole. The crystal structures of domain-swapped chimeras were determined and used to construct a model of Legionella RalF with a RMSD of less than 1Å with the crystal structure, which validated the use of this approach to build a model of Rickettsia RalF. In the Rickettsia RalF model, sequence differences in the capping domain that target it to specific membranes are accommodated by a shift of the entire domain with respect to the Sec7 domain. However, local sequence changes also give rise to an artifactual salt bridge in one of the chimeras, which likely explains why this chimera is recalcitrant to activation. These findings highlight the structural plasticity whereby chimeras can be engineered, but also underline that unpredictable differences can modify their biochemical responses. PMID:25498244

Folly-Klan, Marcia; Sancerne, Bastien; Alix, Eric; Roy, Craig R; Cherfils, Jacqueline; Campanacci, Valérie

2015-02-01

109

Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs.  

Science.gov (United States)

Rickettsia massiliae, belonging to the spotted fever group of Rickettsia, is a human pathogen causing a similar course of disease to that caused by R. conorii, the originally recognized etiologic agent of Mediterranean spotted fever. In view of this similarity, we performed an ultrastructural study of R. massiliae in organs of Rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses. Adult R. massiliae-infected Rh. sanguineus ticks were fed on uninfected Hartley strain guinea pigs, and five females were collected daily throughout their feeding period up to day 6, and analyzed by quantitative real-time PCR and electron microscopy. An increase in rickettsial content was observed in the salivary glands, particularly in the first two days of feeding, and a plateau was observed between days 3 and 6. Rickettsial organisms were observed in all tick organs analyzed, in higher numbers in the fed state, and statistically significant differences were observed in measurements of the periplasmic layer of R. massiliae in salivary glands of fed and unfed Rh. sanguineus ticks, with increased thickness in the former case. This study provides insight into the interface between R. massiliae and Rh. sanguineus ticks, highlighting the need for analysis of R. massiliae to fully ascertain its place as an important pathogenic agent of a spotted fever rickettsiosis. PMID:25108779

Milhano, Natacha; Popov, Vsevolod; Vilhena, Manuela; Bouyer, Donald H; de Sousa, Rita; Walker, David H

2014-10-01

110

Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas.  

OpenAIRE

The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both ...

Azad, A. F.; Sacci, J. B.; Nelson, W. M.; Dasch, G. A.; Schmidtmann, E. T.; Carl, M.

1992-01-01

111

Survey of rickettsiae in humans, dogs, horses, and ticks in Northern Paraná, BrazilLevantamento de riquétsias em humanos, cães, cavalos e carrapatos no Norte do Paraná, Brasil  

Directory of Open Access Journals (Sweden)

Full Text Available Brazilian Spotted Fever is a disease caused by Rickettsia rickettsii, and is transmitted to humans and animals by Amblyomma spp. The objective of this work was to study the epidemiology of spotted fever group rickettsiae in rural areas of Northern Parana. In Alvorada do Sul municipality, 88 humans, 83 dogs, and 18 horses were sampled, and in Arapongas municipality, 138 humans, 90 dogs and 18 horses were studied. All the sera were tested by IFA in which R. rickettsii and R. parkeri were used as antigens, considering titers ? 64 positive. Ticks collected from dogs and horses were tested by PCR. In Alvorada do Sul, 24% and 16.1% of humans, 55.6% and 22.2% of horses and, 22.9% and 18.1% of dogs were seropositive for R rickettsii and R. parkeri, respectively. In Arapongas, 9.4% and 4.3% of the humans, 5.6% and 5.6% of horses and, 13.3% and 12.2% of the dogs were seropositive for R. rickettsii and R. parkeri, respectively. PCR detected seven ticks with gltA sequences that showed similarity with R. bellii. The presence of antibodies to R. parkeri and R. rickettsii in dogs, horses and humans demonstrates a potential risk for spotted fever group rickettsiae in these areas.Febre Maculosa Brasileira é uma doença causada por Rickettsia rickettsii, e é transmitida para humanos e animais por Amblyomma spp. O objetivo deste trabalho foi estudar a epidemiologia de riquétsias do grupo da febre em áreas rurais do Norte do Paraná. No município de Alvorada do Sul, 88 pessoas, 83 cães e 18 cavalos foram amostrados, e no município de Arapongas, 138 seres humanos, 90 cães e 18 cavalos foram estudados. Todos os soros foram testados por IFI com R. rickettsii e R. parkeri como antígenos, considerando-se os títulos ? 64 positivos. Carrapatos coletados de cães e cavalos foram testados por PCR. Em Alvorada do Sul, 24% e 16,1% dos seres humanos, 55,6% e 22,2% de cavalos e, 22,9% e 18,1% de cães foram soropositivos para R. rickettsii e R. parkeri, respectivamente. Em Arapongas, 9,4% e 4,3% dos seres humanos, 5,6% e 5,6% de cavalos e, 13,3% e 12,2% dos cães foram soropositivos para R. rickettsii e R. parkeri, respectivamente. A PCR detectou 7 carrapatos com seqüências gltA que mostrou semelhança com R. bellii. A presença de anticorpos para R. rickettsii e R. parkeri em cães, cavalos e seres humanos demonstra um risco potencial para riquétsias do grupo da febre maculosa nestas áreas.

John Stephen Dumler

2011-10-01

112

Infection of Amblyomma ovale by Rickettsia sp. strain Atlantic rainforest, Colombia.  

Science.gov (United States)

Our goal was to understand rickettsial spotted fevers' circulation in areas of previous outbreaks reported from 2006 to 2008 in Colombia. We herein present molecular identification and isolation of Rickettsia sp. Atlantic rainforest strain from Amblyomma ovale ticks, a strain shown to be pathogenic to humans. Infected ticks were found on dogs and a rodent in Antioquia and Córdoba Provinces. This is the first report of this rickettsia outside Brazil, which expands its known range considerably. PMID:25090976

Londoño, Andrés F; Díaz, Francisco J; Valbuena, Gustavo; Gazi, Michal; Labruna, Marcelo B; Hidalgo, Marylin; Mattar, Salim; Contreras, Verónica; Rodas, Juan D

2014-10-01

113

Plasmids of the pRM/pRF Family Occur in Diverse Rickettsia Species? †  

OpenAIRE

The recent discoveries of the pRF and pRM plasmids of Rickettsia felis and R. monacensis have contravened the long-held dogma that plasmids are not present in the bacterial genus Rickettsia (Rickettsiales; Rickettsiaceae). We report the existence of plasmids in R. helvetica, R. peacockii, R. amblyommii, and R. massiliae isolates from ixodid ticks and in an R. hoogstraalii isolate from an argasid tick. R. peacockii and four isolates of R. amblyommii from widely separated geographic locations c...

Baldridge, Gerald D.; Burkhardt, Nicole Y.; Felsheim, Roderick F.; Kurtti, Timothy J.; Munderloh, Ulrike G.

2008-01-01

114

Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia  

OpenAIRE

Abstract The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT) was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG)-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and

Rees Robert L; O'Leary Caroline A; Thompson Mary F; Kopp Steven R; Hii Sze-Fui; Traub Rebecca J

2011-01-01

115

Phospholipase A and the interaction of Rickettsia prowazekii and mouse fibroblasts (L-929 cells).  

OpenAIRE

L-929 cells were killed when approximately 50 viable Rickettsia prowazekii organisms per L-cell were centrifuged onto a monolayer. The glycerophospholipids of the L-cell were hydrolyzed to lysophosphatides and free fatty acids. Concomitantly, there was a loss of membrane integrity as shown by release of lactate dehydrogenase and 86Rb and permeability to trypan blue dye. No glycerophospholipid hydrolysis or cytotoxicity occurred when the rickettsiae were inactivated by heat, UV irradiation, N-...

Winkler, H. H.; Miller, E. T.

1982-01-01

116

TolC-Dependent Secretion of an Ankyrin Repeat-Containing Protein of Rickettsia typhi  

OpenAIRE

Rickettsia typhi, the causative agent of murine (endemic) typhus, is an obligate intracellular pathogen with a life cycle involving both vertebrate and invertebrate hosts. In this study, we characterized a gene (RT0218) encoding a C-terminal ankyrin repeat domain-containing protein, named Rickettsia ankyrin repeat protein 1 (RARP-1), and identified it as a secreted effector protein of R. typhi. RT0218 showed differential transcript abundance at various phases of R. typhi intracellular grow...

Kaur, Simran J.; Rahman, M. Sayeedur; Ammerman, Nicole C.; Beier-sexton, Magda; Ceraul, Shane M.; Gillespie, Joseph J.; Azad, Abdu F.

2012-01-01

117

Bartonella clarridgeiae, B. henselae and Rickettsia felis in fleas from Morocco  

OpenAIRE

A total of 554 fleas were collected in the Moroccan Casablanca and Tiznit regions from domesticated animals and ruminants between August 2007 and October 2008 and were tested for the presence of Rickettsia spp. and Bartonella spp. using molecular methods. For the first time in Morocco, we found Rickettsia felis, the agent of flea-borne spotted fever in Ctenocephalides felis; B. henselae, an agent of cat scratch disease; and Bartonella clarridgeiae, a cat pathogen and potentially a human patho...

Boudebouch, N.; Sarih, M.; Beaucournu, J-c; Amarouch, H.; Hassar, M.; Raoult, D.; Parola, P.

2011-01-01

118

THE DISTRIBUTION OF RICKETTSIA IN THE TISSUES OF INSECTS AND ARACHNIDS.  

Science.gov (United States)

In the absence of a satisfactory definition of Rickettsia the observations herein recorded were arbitrarily limited to bacterium-like organisms which are intracellular and Gram-negative. Rickettsia of this type were found in the following species: Amblyomma americana, Amblyomma hebraeum, Boophilus decoloratus, Atomus sp., Casinaria infesta, Chrysopa oculata, Ctenocephalus canis, Dermacentor variabilis, Lepisma saccharina, Lucoppia curviseta, Margaropus annulatus, Margaropus annulatus australis, Ornithodoros turicata, Pulex irritans, Rhipicephalus sanguineus, Rhipicephalus evertsi, and Salticus scenicus. Since intracellular, Gram-negative Rickettsia have been recorded in the literature as existing in Cimex lectularius, Dermacentor venustus, Melophagus ovinus, and Pediculus humanus, the occasional occurrence of such bodies must be conceded in the following groups not closely related phylogenetically: Attidae, Trombidiidae, Argasidae, lxodidae, Cinura, Acanthiidae, Pediculidae, Hippoboscidae, Chrysopidae, Pulicidae, and Ichneumonidae. The species which harbor Rickettsia differ widely in diet and habitat. One such species is insectivorous throughout life, two are insectivorous in larval stages, becoming vegetarian in the adult condition, one is chiefly vegetarian but partakes of some animal products, and two are usually entirely vegetarian; while the remainder subsist wholly upon a diet of mammalian blood. Rickettsia are associated, in only a few cases, with diseases in mammals. The evidence at hand does not lead beyond the conclusion that the Rickettsia mentioned above are true Gram-negative microorganisms, easily distinguishable from mitochondria and all other cytoplasmic and nuclear granulations, rather completely adapted to an intracellular existence, exhibiting in some cases a remarkable degree of host specificity, and often inherited through the eggs. PMID:19868737

Cowdry, E V

1923-03-31

119

Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens.  

Directory of Open Access Journals (Sweden)

Full Text Available The recently sequenced Rickettsia felis genome revealed an unexpected plasmid carrying several genes usually associated with DNA transfer, suggesting that ancestral rickettsiae might have been endowed with a conjugation apparatus. Here we present the genome sequence of Rickettsia bellii, the earliest diverging species of known rickettsiae. The 1,552,076 base pair-long chromosome does not exhibit the colinearity observed between other rickettsia genomes, and encodes a complete set of putative conjugal DNA transfer genes most similar to homologues found in Protochlamydia amoebophila UWE25, an obligate symbiont of amoebae. The genome exhibits many other genes highly similar to homologues in intracellular bacteria of amoebae. We sought and observed sex pili-like cell surface appendages for R. bellii. We also found that R. bellii very efficiently multiplies in the nucleus of eukaryotic cells and survives in the phagocytic amoeba, Acanthamoeba polyphaga. These results suggest that amoeba-like ancestral protozoa could have served as a genetic "melting pot" where the ancestors of rickettsiae and other bacteria promiscuously exchanged genes, eventually leading to their adaptation to the intracellular lifestyle within eukaryotic cells.

2006-05-01

120

Rickettsia felis, an emerging flea-transmitted human pathogen  

Directory of Open Access Journals (Sweden)

Full Text Available Rickettsia felis was first recognised two decades ago and has now been described as endemic to all continents except Antarctica. The rickettsiosis caused by R. felis is known as flea-borne spotted fever or cat-flea typhus. The large number of arthropod species found to harbour R. felis and that may act as potential vectors support the view that it is a pan-global microbe. The main arthropod reservoir and vector is the cat flea, Ctenocephalides felis, yet more than 20 other species of fleas, ticks, and mites species have been reported to harbour R. felis. Few bacterial pathogens of humans have been found associated with such a diverse range of invertebrates. With the projected increase in global temperature over the next century, there is concern that changes to the ecology and distribution of R. felis vectors may adversely impact public health.

Stephen Graves

2011-07-01

121

Detección de Rickettsia spp. en ectoparásitos de animales domésticos y silvestres de la Reserva Natural Privada Cerro Chucantí y comunidades aledañas, Panamá, 2007-2010 / Detection of Rickettsia in ectoparasites of wild and domestic mammals from the Cerro Chucanti private reserve and from neighboring towns, Panamá, 2007-2010  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Introducción. Los ectoparásitos son los principales vectores de rickettsiosis. En Panamá se tienen escasos datos sobre los artrópodos que pudieran considerarse vectores o reservorios. Objetivos. Presentar datos sobre la presencia de Rickettsia spp. en ectoparásitos de fauna silvestre y animales domé [...] sticos en la Reserva Natural Privada Cerro Chucantí y poblados vecinos. Materiales y métodos. Se revisaron 9 personas, 95 mamíferos domésticos y 48 silvestres. Los animales domésticos se examinaron con anuencia del propietario, mientras que la fauna silvestre se capturó con trampas Sherman y Tomahawk. Se extrajeron 21 especies de ectoparásitos: pulgas, piojos, garrapatas y otros ácaros, los cuales se preservaron en etanol al 95 %. Se extrajo material genético de garrapatas y pulgas para ser analizado por técnicas moleculares en la detección de Rickettsia spp. Resultados. Se practicaron 425 reacciones de PCR, de las cuales, 270 resultaron negativas y 155 positivas. De las positivas, 86 amplificaron para el gen gltA (55 % de las positivas); de estos también amplificaron 41 (26 %) para ompA. Se encontró material genético de Rickettsia amblyommii, en garrapatas de caballos (Amblyomma cajennense, Dermacentor nitens), de perros (Rhipicephalus sanguineus) y ninfas de Amblyomma recolectadas en el bosque. Además, se detectó ADN de R. felis en pulgas Ctenocephalides felis de perros. Conclusiones. Se pudo detectar la presencia de R. amblyommii y R. felis en garrapatas y pulgas de animales domésticos de los poblados cercanos a Cerro Chucantí, aun cuando no se pudo encontrar material genético de Rickettsia en ectoparásitos de la fauna silvestre. Abstract in english Introduction. Ectoparasites are the main vectors of rickettsiosis. In Panama, however, limited data are available concerning the arthropod species that serve as vectors or reservoirs. Objectives. Data are presented concerning the presence of Rickettsia in ectoparasites of wildlife and domestic anima [...] ls in the Cerro Chucantí private nature reserve and in neighboring villages. Materials and methods. Nine humans, 95 domestic mammals and 48 wild mammals were examined. Twenty-one species of ectoparasites were obtained, including fleas, lice, ticks and mites. These were preserved in 95% ethanol. Later, the DNA was extracted from the ticks and fleas and analyzed by molecular techniques to detect presence of Rickettsia. Results. Of a total of 425 PCR reactions, 270 were positive for Rickettsia and 155 negative. Among the positive samples, 86 PCR amplified for the gltA gene (55% of positives) and 41 of these also amplified the ompA gene. DNA of Rickettsiaamblyommii was found in horses ticks (Amblyomma cajennense, Dermacentor nitens), dogs ticks (Rhipicephalus sanguineus) and free living nymphs in the forest. Additionally, DNA of R. felis was found in fleas from dogs Ctenocephalides felis. Conclusions. The presence of R. amblyommii and R. felis was detected in ticks and fleas of domestic animals in villages near Cerro Chucanti; however no Rickettsia DNA was found in ectoparasites of non-domestic wildlife.

Sergio, Bermúdez; Roberto, Miranda; Yamitze, Zaldívar; Publio, González; Guido, Berguido; Diomedes, Trejos; Juan M, Pascale; Marcelo, Labruna.

2012-06-01

122

Isolamento de Rickettsia em cultura de células vero Isolation of Rickettsia in vero cell culture  

OpenAIRE

Embora o diagnóstico da febre maculosa baseie-se em sinais e sintomas característicos, o mesmo requer confirmação laboratorial, pois existem alguns diagnósticos diferenciais possíveis como meningococcemia, leptospirose, infecção por enterovírus e febre tifóide. A confirmação laboratorial pode ser feita através da pesquisa de anticorpos específicos, possível somente alguns dias após o aparecimento da doença, através do isolamento do agente em amostras de sangue e/ou biópsia ...

Heloisa Helena Barbosa Melles; Silvia Colombo; Elba Regina Sampaio de Lemos

1999-01-01

123

Wide Dispersal and Possible Multiple Origins of Low-Copy-Number Plasmids in Rickettsia Species Associated with Blood-Feeding Arthropods?  

OpenAIRE

Plasmids are mobile genetic elements of bacteria that can impart important adaptive traits, such as increased virulence or antibiotic resistance. We report the existence of plasmids in Rickettsia (Rickettsiales; Rickettsiaceae) species, including Rickettsia akari, “Candidatus Rickettsia amblyommii,” R. bellii, R. rhipicephali, and REIS, the rickettsial endosymbiont of Ixodes scapularis. All of the rickettsiae were isolated from humans or North and South American ticks. R. parkeri isolates...

Baldridge, Gerald D.; Burkhardt, Nicole Y.; Labruna, Marcelo B.; Pacheco, Richard C.; Paddock, Christopher D.; Williamson, Philip C.; Billingsley, Peggy M.; Felsheim, Roderick F.; Kurtti, Timothy J.; Munderloh, Ulrike G.

2010-01-01

124

Proposal to create subspecies of Rickettsia conorii based on multi-locus sequence typing and an emended description of Rickettsia conorii  

Directory of Open Access Journals (Sweden)

Full Text Available Abstract Background Rickettsiae closely related to the Malish strain, the reference Rickettsia conorii strain, include Indian tick typhus rickettsia (ITTR, Israeli spotted fever rickettsia (ISFR, and Astrakhan fever rickettsia (AFR. Although closely related genotypically, they are distinct serotypically. Using multilocus sequence typing (MLST, we have recently found that distinct serotypes may not always represent distinct species within the Rickettsia genus. We investigated the possibility of classifying rickettsiae closely related to R. conorii as R. conorii subspecies as proposed by the ad hoc committee on reconciliation of approaches to bacterial systematics. For this, we first estimated their genotypic variability by using MLST including the sequencing of 5 genes, of 31 rickettsial isolates closely related to R. conorii strain Malish, 1 ITTR isolate, 2 isolates and 3 tick amplicons of AFR, and 2 ISFR isolates. Then, we selected a representative of each MLST genotype and used multi-spacer typing (MST and mouse serotyping to estimate their degree of taxonomic relatedness. Results Among the 39 isolates or tick amplicons studied, four MLST genotypes were identified: i the Malish type; ii the ITTR type; iii the AFR type; and iv the ISFR type. Among these four MLST genotypes, the pairwise similarity in nucleotide sequence varied from 99.8 to 100%, 99.4 to 100%, 98.2 to 99.8%, 98.4 to 99.8%, and 99.2 to 99.9% for 16S rDNA, gltA, ompA, ompB, and sca4 genes, respectively. Representatives of the 4 MLST types were also classified within four types using MST genotyping as well as mouse serotyping. Conclusion Although homogeneous genotypically, strains within the R. conorii species show MST genotypic, serotypic, and epidemio-clinical dissimilarities. We, therefore, propose to modify the nomenclature of the R. conorii species through the creation of subspecies. We propose the names R. conorii subsp. conorii subsp. nov. (type strain = Malish, ATCC VR-613, R. conorii subspecies indica subsp. nov. (type strain = ATCC VR-597, R. conorii subspecies caspia subsp. nov. (type strain = A-167, and R. conorii subspecies israelensis subsp. nov. (type strain = ISTT CDC1. The description of R. conorii is emended to accomodate the four subspecies.

Raoult Didier

2005-03-01

125

Lysis of typhus-group rickettsia-infected targets by lymphokine activated killers  

International Nuclear Information System (INIS)

The authors recently described a subset of OKT8, OKT3-positive lymphocytes from typhus-group rickettsia immune individuals which were capable of lysing autologous PHA-blasts or Epstein-Barr virus transformed B cells (LCL) infected with typhus-group rickettsiae. In order to determine if killing by these effectors was HLA-restricted, they stimulated peripheral blood mononuclear cells (PBMC) from typhus-group rickettsia immune individuals in vitro with typhus-group rickettsia-derived antigen for one week and then measured lysis of autologous LCL or HLA-mismatched LCL in a 4-6 hour Cr51-release assay. There was significant lysis of both the autologous and the HLA-mismatched infected targets as compared to the corresponding uninfected targets. Since this suggested that the effectors were lymphokine activated killers (LAK) rather than cytotoxic T lymphocytes, they then tested this hypothesis by stimulating PBMC from both immune and non-immune individuals in vitro for one week with purified interleukin 2 and measuring lysis of infected, autologous LCL. PBMC thus treated, from both immune and non-immune individuals, were capable of significantly lysing autologous, infected LCL as compared to the non-infected control. They therefore conclude that targets infected with typhus-group rickettsiae are susceptible to lysis to LAK

126

Lysis of typhus-group rickettsia-infected targets by lymphokine activated killers  

Energy Technology Data Exchange (ETDEWEB)

The authors recently described a subset of OKT8, OKT3-positive lymphocytes from typhus-group rickettsia immune individuals which were capable of lysing autologous PHA-blasts or Epstein-Barr virus transformed B cells (LCL) infected with typhus-group rickettsiae. In order to determine if killing by these effectors was HLA-restricted, they stimulated peripheral blood mononuclear cells (PBMC) from typhus-group rickettsia immune individuals in vitro with typhus-group rickettsia-derived antigen for one week and then measured lysis of autologous LCL or HLA-mismatched LCL in a 4-6 hour Cr/sup 51/-release assay. There was significant lysis of both the autologous and the HLA-mismatched infected targets as compared to the corresponding uninfected targets. Since this suggested that the effectors were lymphokine activated killers (LAK) rather than cytotoxic T lymphocytes, they then tested this hypothesis by stimulating PBMC from both immune and non-immune individuals in vitro for one week with purified interleukin 2 and measuring lysis of infected, autologous LCL. PBMC thus treated, from both immune and non-immune individuals, were capable of significantly lysing autologous, infected LCL as compared to the non-infected control. They therefore conclude that targets infected with typhus-group rickettsiae are susceptible to lysis to LAK.

Carl, M.; Dasch, G.A.

1986-03-01

127

The Facultative Symbiont Rickettsia Protects an Invasive Whitefly Against Entomopathogenic Pseudomonas syringae Strains.  

Science.gov (United States)

Facultative endosymbionts can benefit insect hosts in a variety of ways, including context dependent roles such as providing defense against pathogens. The role of some symbionts in defense may be overlooked, however, when pathogen infection is transient, sporadic, or asymptomatic. The facultative endosymbiont Rickettsia increases the fitness of the sweet potato whitefly (Bemisia tabaci) in some populations through mechanisms that are not yet understood. In this study we investigated the role of Rickettsia in mediating the interaction between the sweet potato whitefly and Pseudomonas syringae, a common environmental bacterium, some strains of which are pathogenic to aphids. Our results show that P. syringae multiplies within whiteflies leading to host death and that whiteflies infected with Rickettsia show a decreased rate of death due to P. syringae. Experiments using plants coated with P. syringae confirmed that whiteflies can acquire the bacteria at a low rate while feeding, leading to increased mortality, particularly when the whiteflies are not infected with Rickettsia. These results suggest that P. syringae may affect whitefly populations in nature and that Rickettsia can ameliorate this effect. This study highlights the possible importance of interactions among opportunistic environmental pathogens and endosymbionts of insects. PMID:25217020

Hendry, Tory A; Hunter, Martha S; Baltrus, David A

2014-09-12

128

Identification of rickettsiae from wild rats and cat fleas in Malaysia.  

Science.gov (United States)

Rickettsioses are emerging zoonotic diseases reported worldwide. In spite of the serological evidence of spotted fever group rickettsioses in febrile patients in Malaysia, limited studies have been conducted to identify the animal reservoirs and vectors of rickettsioses. This study investigated the presence of rickettsiae in the tissue homogenates of 95 wild rats and 589 animal ectoparasites. Using PCR assays targeting the citrate synthase gene (gltA), rickettsial DNA was detected in the tissue homogenates of 13 (13.7%) wild rats. Sequence analysis of the gltA amplicons showed 98.6-100% similarity with those of Rickettsia honei/R. conorii/R. raoultii (Rickettsiales: Rickettsiaceae). Sequence analysis of outer membrane protein A gene (ompA) identified Rickettsia sp. TCM1 strain from two rats. No rickettsia was detected from Laelaps mites, Rhipicephalus sanguineus and Haemaphysalis bispinosa ticks, and Felicola subrostratus lice in this study. R. felis was identified from 32.2% of 177 Ctenocephalides felis fleas. Sequence analysis of the gltA amplicons revealed two genotypes of R. felis (Rf31 and RF2125) in the fleas. As wild rats and cat fleas play an important role in the enzoonotic maintenance of rickettsiae, control of rodent and flea populations may be able to reduce transmission of rickettsioses in the local setting. PMID:25171613

Tay, S T; Mokhtar, A S; Low, K C; Mohd Zain, S N; Jeffery, J; Abdul Aziz, N; Kho, K L

2014-08-01

129

Estrés oxidativo en el glaucoma primario de ángulo abierto. Prevención de la ceguera por glaucoma.  

OpenAIRE

Con este estudio hemos pretendido demostrar que los mecanismos de estrés oxidativo y nitrosativo están relacionados con el glaucoma primario de ángulo abierto (GPAA), y que los neurotransmisores están implicados en las señales que relacionan la hipertensión ocular (HTO) con la muerte por apoptosis de las células ganglionares de la retina y la pérdida subsecuente de las fibras del nervio óptico, lo que conduce a la atrofia óptica y a la ceguera glaucomatosa.Este estudio de casos y co...

Zano?n Moreno, Vicente

2008-01-01

130

Tick cell culture isolation and growth of Rickettsia raoultii from Dutch Dermacentor reticulatus ticks  

Science.gov (United States)

Tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. In an attempt to derive continuous Dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks originally collected as unfed adults in The Netherlands and maintained for up to 16 months. After several months, it became evident that cells in the primary cultures were infected with a Rickettsia-like intracellular organism. Supernatant medium containing some D. reticulatus cells was inoculated into cultures of 2 Rhipicephalus (Boophilus) microplus cell lines, BME/CTVM2 and BME/CTVM23, where abundant growth of the bacteria occurred intracellularly on transfer to both cell lines. Bacterial growth was monitored by light (live, inverted microscope, Giemsa-stained cytocentrifuge smears) and transmission electron microscopy revealing heavy infection with typical intracytoplasmic Rickettsia-like bacteria, not present in uninfected cultures. DNA was extracted from bacteria-infected and uninfected control cultures, and primers specific for Rickettsia 16S rRNA, ompB, and sca4 genes were used to generate PCR products that were subsequently sequenced. D. reticulatus primary cultures and both infected tick cell lines were positive for all 3 Rickettsia genes. Sequencing of PCR products revealed 99–100% identity with published Rickettsia raoultii sequences. The R. raoultii also grew abundantly in the D. nitens cell line ANE58, poorly in the D. albipictus cell line DALBE3, and not at all in the D. andersoni cell line DAE15. In conclusion, primary tick cell cultures and cell lines are useful systems for isolation and propagation of fastidious tick-borne microorganisms. In vitro isolation of R. raoultii from Dutch D. reticulatus confirms previous PCR-based detection in field ticks, and presence of the bacteria in the tick eggs used to initiate the primary cultures confirms that transovarial transmission of this Rickettsia occurs. PMID:23140894

Alberdi, M. Pilar; Nijhof, Ard M.; Jongejan, Frans; Bell-Sakyi, Lesley

2012-01-01

131

Isolation and Identification of Rickettsia massiliae from Rhipicephalus sanguineus Ticks Collected in Arizona  

OpenAIRE

Twenty Rhipicephalus sanguineus ticks collected in eastern Arizona were tested by PCR assay to establish their infection rate with spotted fever group rickettsiae. With a nested PCR assay which detects a fragment of the Rickettsia genus-specific 17-kDa antigen gene (htrA), five ticks (25%) were found to contain rickettsial DNA. One rickettsial isolate was obtained from these ticks by inoculating a suspension of a triturated tick into monolayers of Vero E6 monkey kidney cells and XTC-2 clawed ...

Eremeeva, Marina E.; Bosserman, Elizabeth A.; Demma, Linda J.; Zambrano, Maria L.; Blau, Dianna M.; Dasch, Gregory A.

2006-01-01

132

Survey for hantaviruses, tick-borne encephalitis virus, and Rickettsia spp. in small rodents in Croatia.  

Science.gov (United States)

In Croatia, several rodent- and vector-borne agents are endemic and of medical importance. In this study, we investigated hantaviruses and, for the first time, tick-borne encephalitis virus (TBEV) and Rickettsia spp. in small wild rodents from two different sites (mountainous and lowland region) in Croatia. In total, 194 transudate and tissue samples from 170 rodents (A. flavicollis, n=115; A. agrarius, n=2; Myodes glareolus, n=53) were tested for antibodies by indirect immunofluorescence assays (IIFT) and for nucleic acids by conventional (hantaviruses) and real-time RT-/PCRs (TBEV and Rickettsia spp.). A total of 25.5% (24/94) of the rodents from the mountainous area revealed specific antibodies against hantaviruses. In all, 21.3% (20/94) of the samples from the mountainous area and 29.0% (9/31) from the lowland area yielded positive results for either Puumala virus (PUUV) or Dobrava-Belgrade virus (DOBV) using a conventional RT-PCR. All processed samples (n=194) were negative for TBEV by IIFT or real-time RT-PCR. Serological evidence of rickettsial infection was detected in 4.3% (4/94) rodents from the mountainous region. Another 3.2% (3/94) rodents were positive for Rickettsia spp. by real-time PCR. None of the rodents (n=76) from the lowland area were positive for Rickettsia spp. by real-time PCR. Dual infection of PUUV and Rickettsia spp. was found in one M. glareolus from the mountainous area by RT-PCR and real-time PCR, respectively. To our knowledge, this is the first detection of Rickettsia spp. in small rodents from Croatia. Phylogenetic analyses of S- and M-segment sequences obtained from the two study sites revealed well-supported subgroups in Croatian PUUV and DOBV. Although somewhat limited, our data showed occurrence and prevalence of PUUV, DOBV, and rickettsiae in Croatia. Further studies are warranted to confirm these data and to determine the Rickettsia species present in rodents in these areas. PMID:24866325

Svoboda, Petra; Dobler, Gerhard; Markoti?, Alemka; Kurolt, Ivan-Christian; Speck, Stephanie; Habuš, Josipa; Vucelja, Marko; Krajinovi?, Lidija Cvetko; Tadin, Ante; Margaleti?, Josip; Essbauer, Sandra

2014-07-01

133

Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia  

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Full Text Available Abstract The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and gltA genes were amplified from the blood of 3 dogs. This study is the first report of R. felis infection in indigenous community dogs in NT.

Rees Robert L

2011-10-01

134

Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia  

OpenAIRE

The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT) was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG)-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and gltA genes were amplified from the blood of 3 dogs. This study is ...

Hii, Sze-fui; Kopp, Steven R.; Thompson, Mary F.; O Leary, Caroline A.; Rees, Robert L.; Traub, Rebecca J.

2011-01-01

135

Comparative genomics of Rickettsia prowazekii Madrid E and Breinl strains.  

Science.gov (United States)

Rickettsia prowazekii, the causative agent of epidemic typhus, has been responsible for millions of human deaths. Madrid E is an attenuated strain of R. prowazekii, while Breinl is a virulent strain. The genomic DNA sequence of Madrid E has recently been published. To study the genomic variations between Madrid E (reference) and Breinl (test) DNAs, cohybridization experiments were performed on a DNA microarray containing all 834 protein-coding genes of Madrid E. Of the 834 genes assessed, 24 genes showed 1.5- to 2.0-fold increases in hybridization signals in Breinl DNA compared to Madrid E DNA, indicating the presence of genomic variations in approximately 3% of the total genes. Eighteen of these 24 genes are predicted to be involved in different functions. Southern blot analysis of five genes, virB4, ftsK, rfbE, lpxA, and rpoH, suggested the presence of an additional paralog(s) in Breinl, which might be related to the observed increase in hybridization signals. Studies by real-time reverse transcription-PCR revealed an increase in expression of the above-mentioned five genes and five other genes. In addition to the elevated hybridization signals of 24 genes observed in the Breinl strain, one gene (rp084) showed only 1/10 the hybridization signal of Madrid E. Further analysis of this gene by PCR and sequencing revealed a large deletion flanking the whole rp084 gene and part of the rp083 gene in the virulent Breinl strain. The results of this first rickettsial DNA microarray may provide some important information for the elucidation of pathogenic mechanisms of R. prowazekii. PMID:14702324

Ge, Hong; Chuang, Yao-Yu Eric; Zhao, Shuping; Tong, Min; Tsai, Mong-Hsun; Temenak, Joseph J; Richards, Allen L; Ching, Wei-Mei

2004-01-01

136

Synanthropic Birds Associated with High Prevalence of Tick-Borne Rickettsiae and with the First Detection of Rickettsia aeschlimannii in Hungary  

OpenAIRE

The aim of this study was to analyze synanthropic birds as risk factors for introducing ticks and tick-borne pathogens into human settlements, with an emphasis on rickettsiae. Altogether 184 subadult ticks were found on 5846 birds. Tick infestation was most prevalent during the spring. In this sample group the majority of ticks were molecularly identified as Ixodes ricinus, and three individuals collected from the European robin as Hyalomma marginatum marginatum. The latter is the first molec...

Hornok, Sa?ndor; Cso?rgo?, Tibor; La Fuente, Jose?; Gyuranecz, Miklo?s; Privigyei, Csaba; Meli, Marina L.; Kreizinger, Zsuzsa; Go?nczi, Eniko?; Ferna?ndez Mera, Isabel G.; Hofmann-lehmann, Regina

2013-01-01

137

Detection of Rickettsia helvetica in Ixodes ricinus ticks collected from Pyrenean chamois in France.  

Science.gov (United States)

Seventy-one Ixodes ricinus ticks collected from Pyrenean chamois (Rupicapra pyrenaica) in the French Pyrenees were tested by real-time polymerase chain reaction to detect the presence of Rickettsia and Bartonella. Four ticks (6%) were positive for R. helvetica. The chamois carries infected ticks, and this enables the dissemination throughout the environment with this bacterium, a potential human pathogen. PMID:23141107

Davoust, Bernard; Socolovschi, Cristina; Revelli, Paul; Gibert, Philippe; Marié, Jean-Lou; Raoult, Didier; Parola, Philippe

2012-12-01

138

Rickettsia slovaca in Dermacentor marginatus ticks and Tick Borne Lymphadenopathy in Tuscany, central Italy  

OpenAIRE

Of 263 patients in Tuscany, Italy, from whom ticks were removed during July 2005–May 2007, fi ve showed signs of tick-borne lymphadenopathy. Of the ticks, 17 were Dermacentor marginatus; 6 (35.3%) of these were identifi ed by sequence analysis as containing Rickettsia slovaca. Tickborne lymphadenopathy occurs in this area.

Mannelli, Alessandro; Tomassone, Laura; Bertolotti, Luigi

2008-01-01

139

Rickettsia infection in five areas of the state of São Paulo, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus [...] , Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi), and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus) were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii), some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.

Maurício C, Horta; Marcelo B, Labruna; Adriano, Pinter; Pedro M, Linardi; Teresinha T S, Schumaker.

2007-11-01

140

Rickettsia typhi and R. felis in Rat Fleas (Xenopsylla cheopis), Oahu, Hawaii  

OpenAIRE

Rickettsia typhi (prevalence 1.9%) and R. felis (prevalence 24.8%) DNA were detected in rat fleas (Xenopsylla cheopis) collected from mice on Oahu Island, Hawaii. The low prevalence of R. typhi on Oahu suggests that R. felis may be a more common cause of rickettsiosis than R. typhi in Hawaii.

Eremeeva, Marina E.; Warashina, Wesley R.; Sturgeon, Michele M.; Buchholz, Arlene E.; Olmsted, Gregory K.; Park, Sarah Y.; Effler, Paul V.; Karpathy, Sandor E.

2008-01-01

141

Rickettsia infection in five areas of the state of São Paulo, Brazil  

Directory of Open Access Journals (Sweden)

Full Text Available This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus, Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi, and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii, some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.

Maurício C Horta

2007-11-01

142

Characterization of a new antigenic type, Kuroki, of Rickettsia tsutsugamushi isolated from a patient in Japan.  

OpenAIRE

The Kuroki strain of Rickettsia tsutsugamushi, isolated from a patient in Kyushu, Japan, has a major, type-specific antigenic polypeptide which is distinct from the prototype strains in size (58 kilodaltons), in antigenicity, and in its cleavage pattern with N-chlorosuccinimide. These results indicate that Kuroki is another antigenic type of R. tsutsugamushi.

Ohashi, N.; Tamura, A.; Sakurai, H.; Yamamoto, S.

1990-01-01

143

Methodology for a medical expert system on fuzzy analog ganglionar lattices. Non-approximate reasoning with multiple antecendents of different relative importance and limited uncertainty.  

Science.gov (United States)

This work presents an Expert System based on fuzzy analog ganglionar lattices. Its reasoning scheme is designed analogously to the expert's mental organization and it is realized on an (analog) operator called the ganglionar lattice. It is a connectionist system that uses the medical knowledge to define its architecture. The operator evokes some similarities to higher order neural networks and performs as the knowledge base and inference engine of the expert system, in a unified manner. A main feature of this operator is that it exhibits the variables corresponding to all intermediate concepts identified by the expert; this characteristic is shown to be most valuable for assessing, explicating and prospecting in medical applications. Further, it is capable of (i) evaluating a consequent for a variety of non-approximate reasonings with multiple antecendents of different relative importance under limited uncertainty; (ii) explicating the conclusions at different levels of abstraction to suit the user; and (iii) prospecting for the best 'a priori' sequence of unevaluated antecedents, from which to choose following tests. These procedures are based on the objective criterion of the consequent's uncertainty decrease (entropy). All results are produced in numerical form and may be translated into restricted natural language. A simple example of this technology is fully developed. Finally the method's potentials are discussed for future applications. PMID:8776711

Holzmann, C A; Ehijo, A; Perez, C A

144

Pesquisa de Rickettsia spp em carrapatos Amblyomma cajennense e Amblyomma dubitatum no Estado de São Paulo Survey of Rickettsia spp in the ticks Amblyomma cajennense and Amblyomma dubitatum in the State of São Paulo  

Directory of Open Access Journals (Sweden)

Full Text Available Foi pesquisada a presença de riquétsias em 3.545 carrapatos Amblyomma cajennense e 2.666 Amblyomma dubitatum. Através do teste de hemolinfa, reação em cadeia pela polimerase e isolamento de rickettsia em cultivo celular, todos os Amblyomma cajennense foram negativos, sendo que 634 (23,8% Amblyomma dubitatum mostraram-se infectados com Rickettsia bellii.The presence of rickettsial infection was surveyed in 3,545 Amblyomma cajennense ticks and 2,666 Amblyomma dubitatum ticks. Using the hemolymph test, polymerase chain reaction and isolation of Rickettsia in cell cultures, all of the Amblyomma cajennense were negative, whereas 634 (23.8% of the Amblyomma dubitatum ticks were shown to be infected with Rickettsia bellii.

Richard Campos Pacheco

2009-06-01

145

Detecção de riquétsias em carrapatos do gênero Amblyomma (Acari: Ixodidae) coletados em parque urbano do município de Campinas, SP / Rickettsiae detection in Amblyomma ticks (Acari: Ixodidae) collected in the urban area of Campinas city, SP  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O Município de Campinas situa-se em região endêmica para febre maculosa brasileira do Estado de São Paulo, onde vários casos desta doença vem ocorrendo. Capivaras têm sido associadas ao ciclo dessa riquetsiose por apresentarem sorologia positiva e serem hospedeiras de carrapatos Amblyomma spp princi [...] pais vetores da doença. Carrapatos foram coletados no parque urbano do Lago do Café, Campinas, SP, local associado a casos humanos suspeitos de febre maculosa brasileira, sobre a vegetação e das capivaras ali presentes, e pesquisados quanto à presença de riquétsias pela reação em cadeia da polimerase e pelo teste de hemolinfa. Adultos de Amblyomma cajennense e Amblyomma cooperi albergavam Rickettsia bellii, não patogênica, identificada pela análise das seqüências de nucleotídeos do gene gltA, porém, não foram constatadas riquétsias do Grupo da Febre Maculosa. Estes resultados associados à ausência de um isolado de riquétsias do Grupo da Febre Maculosa de capivaras indicam que seu papel, enquanto reservatório, necessita de maior investigação. Abstract in english The city of Campinas is located in an endemic area for brazilian spotted fever in São Paulo State, where several cases have recently occurred. Capybaras have been associated with the cycle of this disease, for they present positive serology and serve as host for ticks of the genus Amblyomma, the mai [...] n vectors of brazilian spotted fever. Ticks were colleted both from Capybaras and from the vegetation in the city park Lago do Café, located in the urban area of Campinas city, SP, a site associated with suspected human cases of brazilian spotted fever. The ticks collected were examinaded for the presence of rickettsiae using polymerase chain reaction and the haemolymph test. Through analysis of the gene gltA nucleotide sequence, adults of Amblyomma cajennense and Amblyomma cooperi were found to be infected with the non pathogenic Rickettsia bellii. However, no rickettsiae of the spotted fever group were detected. These results indicate that the role of capybaras as reservoirs of rickettsiae of the Spotted Fever group is still uncertain and further studies are required.

Dora Amparo, Estrada; Teresinha Tizu Sato, Schumaker; Celso Eduardo de, Souza; Elias José, Rodrigues Neto; Arício Xavier, Linhares.

2006-02-01

146

Molecular detection of Rickettsia felis and Bartonella henselae in dog and cat fleas in Central Oromia, Ethiopia.  

Science.gov (United States)

Fleas are important vectors of several Rickettsia and Bartonella spp. that cause emerging zoonotic diseases worldwide. In this study, 303 fleas collected from domestic dogs and cats in Ethiopia and identified morphologically as Ctenocephalides felis felis, C. canis, Pulex irritans, and Echidnophaga gallinacea were tested for Rickettsia and Bartonella DNA by using molecular methods. Rickettsia felis was detected in 21% of fleas, primarily C. felis, with a similar prevalence in fleas from dogs and cats. A larger proportion of flea-infested dogs (69%) than cats (37%) harbored at least one C. felis infected with R. felis. Rickettsia typhi was not detected. Bartonella henselae DNA was detected in 6% (2 of 34) of C. felis collected from cats. Our study highlights the likelihood of human exposure to R. felis, an emerging agent of spotted fever, and B. henselae, the agent of cat-scratch disease, in urban areas in Ethiopia. PMID:24445204

Kumsa, Bersissa; Parola, Philippe; Raoult, Didier; Socolovschi, Cristina

2014-03-01

147

Identification of Rickettsia africae and Wolbachia sp. in Ceratophyllus garei Fleas from Passerine Birds Migrated from Africa  

OpenAIRE

The aim of the study was to reveal new aspects of the role of flea vector taken from migratory birds by screening of specimens with molecular biological methods. A field study was done in fishponds in Slovakia. Actually, 47 fleas were collected from reed warblers (Acrocephalus scirpaceus) and their nests. DNA was extracted and analyzed for representatives of the orders Rickettsiales. A rickettsia that shares 99.7% of identity by gltA gene with Rickettsia africae was identified in Ceratophyllu...

Sekeyova?, Zuzana; Mediannikov, Oleg; Roux, Ve?ronique; Subramanian, Geetha; S?pitalska?, Eva; Kristofi?k, Jano; Darolova?, Alz?beta; Raoult, Didier

2012-01-01

148

Effects of dorsal root ganglion destruction by adriamycin in patients with postherpetic neuralgia / Efeitos da destruição da raiz dorsal ganglionar pela adriamicina em pacientes com neuralgia pós-herpética  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese OBJETIVO: Investigar os efeitos da destruição da raiz dorsal ganglionar em pacientes com neuralgia pós-herpética. MÉTODOS: Setenta e dois pacientes selecionados com neuralgia pós-herpética foram randomicamente distribuídos em dois grupos (n=36). Grupo A foi o grupo controle (tratado por injeção) e o [...] grupo B foi o grupo com destruição da raiz dorsal do gânglio pela adriamicina. Os escores da Escala Analógica Visual (VAS), SAS, SF-MPQ escores, efeitos clínicos e segurança terapêutica foram avaliados as antes da terapia, uma semana, três e seis meses após a terapia. Quarenta e quatro pacientes foram avaliados pela análise de intenção-em-tratar. RESULTADOS: A média dos escores de dor na escala de Likert foi significativamente reduzida em cada ponto no grupo B. Pacientes no grupo B relataram efetividade clínica aos seis meses com excelente resposta (16), boa resposta (12), melhora mais insatisfatória ou sem modificações (8). Escores VAS a cada tempo após o procedimento foram melhores em comparação ao pré-operatório. No grupo A não foi observada diferença significativa. Pacientes mostraram melhora nos escores de dormir no grupo B. Houve diferença significante no T2 no grupo A que T1. Não houve diferença significante no grupo A nos tempos T3 e T4 após a cirurgia em relação a antes. Comparação entre os grupos: houve diferença significante entre os grupos A e B a cada tempo após a cirurgia. CONCLUSÕES: A destruição da raiz dorsal ganglionar pela adriamicina sob perspectiva guiada pelo C-arm, a cirurgia pontual foi acurada sem qualquer reação adversa ou complicação séria, que pode efetivamente aliviar a dor em pacientes com neuralgia pós-herpética, mas os efeitos de longo prazo necessitam mais estudos. Abstract in english PURPOSE: To investigate the effects of dorsal root ganglion destruction in patients with postherpetic neuralgia (PHN). METHODS: Seventy-two patients with PHN selected were randomly divided into two groups (n=36). Group A was the control group (treated by injection) and group B was the group of dorsa [...] l root ganglion destruction by adriamycin. Visual analog scale scores (VAS), SAS, SF-MPQ scores. Clinical effects and therapy safety were evaluated before therapy, one week, three and six months after therapy. Forty-four patients were available for intention-to-treat analysis. RESULTS: The average pain scores on the Likert scale were significantly reduced at each point in group B. Patients in group B reported clinical effectiveness at six months as excellent response, good response, improved but unsatisfactory or unchanged 16, 12 and 8.VAS scores at each time point after the operation were lower than that before operation and in group A, there was significant difference. Patients showed significant improvement in sleep scores in group B. There was significant difference at T2 in group A than T1. There was no significant difference in group A at T3, T4 after the operation than that before operation. Between group comparison: there was significant difference between group A and group B at each time point after the operation. CONCLUSIONS: Dorsal root ganglion destruction by adriamycin under guidance of C-arm perspective, the puncture operation was accurate without any adverse reaction or serious complications, which could effectively relieve pain of patients with postherpetic neuralgia, but the long-term effects needed further study.

He, Chun-jing; Luo, yi-ran; Nie, hao-xiong.

2012-06-01

149

Effects of dorsal root ganglion destruction by adriamycin in patients with postherpetic neuralgia Efeitos da destruição da raiz dorsal ganglionar pela adriamicina em pacientes com neuralgia pós-herpética  

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Full Text Available PURPOSE: To investigate the effects of dorsal root ganglion destruction in patients with postherpetic neuralgia (PHN. METHODS: Seventy-two patients with PHN selected were randomly divided into two groups (n=36. Group A was the control group (treated by injection and group B was the group of dorsal root ganglion destruction by adriamycin. Visual analog scale scores (VAS, SAS, SF-MPQ scores. Clinical effects and therapy safety were evaluated before therapy, one week, three and six months after therapy. Forty-four patients were available for intention-to-treat analysis. RESULTS: The average pain scores on the Likert scale were significantly reduced at each point in group B. Patients in group B reported clinical effectiveness at six months as excellent response, good response, improved but unsatisfactory or unchanged 16, 12 and 8.VAS scores at each time point after the operation were lower than that before operation and in group A, there was significant difference. Patients showed significant improvement in sleep scores in group B. There was significant difference at T2 in group A than T1. There was no significant difference in group A at T3, T4 after the operation than that before operation. Between group comparison: there was significant difference between group A and group B at each time point after the operation. CONCLUSIONS: Dorsal root ganglion destruction by adriamycin under guidance of C-arm perspective, the puncture operation was accurate without any adverse reaction or serious complications, which could effectively relieve pain of patients with postherpetic neuralgia, but the long-term effects needed further study.OBJETIVO: Investigar os efeitos da destruição da raiz dorsal ganglionar em pacientes com neuralgia pós-herpética. MÉTODOS: Setenta e dois pacientes selecionados com neuralgia pós-herpética foram randomicamente distribuídos em dois grupos (n=36. Grupo A foi o grupo controle (tratado por injeção e o grupo B foi o grupo com destruição da raiz dorsal do gânglio pela adriamicina. Os escores da Escala Analógica Visual (VAS, SAS, SF-MPQ escores, efeitos clínicos e segurança terapêutica foram avaliados as antes da terapia, uma semana, três e seis meses após a terapia. Quarenta e quatro pacientes foram avaliados pela análise de intenção-em-tratar. RESULTADOS: A média dos escores de dor na escala de Likert foi significativamente reduzida em cada ponto no grupo B. Pacientes no grupo B relataram efetividade clínica aos seis meses com excelente resposta (16, boa resposta (12, melhora mais insatisfatória ou sem modificações (8. Escores VAS a cada tempo após o procedimento foram melhores em comparação ao pré-operatório. No grupo A não foi observada diferença significativa. Pacientes mostraram melhora nos escores de dormir no grupo B. Houve diferença significante no T2 no grupo A que T1. Não houve diferença significante no grupo A nos tempos T3 e T4 após a cirurgia em relação a antes. Comparação entre os grupos: houve diferença significante entre os grupos A e B a cada tempo após a cirurgia. CONCLUSÕES: A destruição da raiz dorsal ganglionar pela adriamicina sob perspectiva guiada pelo C-arm, a cirurgia pontual foi acurada sem qualquer reação adversa ou complicação séria, que pode efetivamente aliviar a dor em pacientes com neuralgia pós-herpética, mas os efeitos de longo prazo necessitam mais estudos.

He Chun-jing

2012-06-01

150

Orientia, rickettsia, and leptospira pathogens as causes of CNS infections in Laos : a prospective study  

DEFF Research Database (Denmark)

BACKGROUND: Scrub typhus (caused by Orientia tsutsugamushi), murine typhus (caused by Rickettsia typhi), and leptospirosis are common causes of febrile illness in Asia; meningitis and meningoencephalitis are severe complications. However, scarce data exist for the burden of these pathogens in patients with CNS disease in endemic countries. Laos is representative of vast economically poor rural areas in Asia with little medical information to guide public health policy. We assessed whether these pathogens are important causes of CNS infections in Laos. METHODS: Between Jan 10, 2003, and Nov 25, 2011, we enrolled 1112 consecutive patients of all ages admitted with CNS symptoms or signs requiring a lumbar puncture at Mahosot Hospital, Vientiane, Laos. Microbiological examinations (culture, PCR, and serology) targeted so-called conventional bacterial infections (Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, S suis) and O tsutsugamushi, Rickettsia typhi/Rickettsia spp, and Leptospira spp infections in blood or cerebrospinal fluid (CSF). We analysed and compared causes and clinical and CSF characteristics between patient groups. FINDINGS: 1051 (95%) of 1112 patients who presented had CSF available for analysis, of whom 254 (24%) had a CNS infection attributable to a bacterial or fungal pathogen. 90 (35%) of these 254 infections were caused by O tsutsugamushi, R typhi/Rickettsia spp, or Leptospira spp. These pathogens were significantly more frequent than conventional bacterial infections (90/1051 [9%] vs 42/1051 [4%]; p<0·0001) by use of conservative diagnostic definitions. CNS infections had a high mortality (236/876 [27%]), with 18% (13/71) for R typhi/Rickettsia spp, O tsutsugamushi, and Leptospira spp combined, and 33% (13/39) for conventional bacterial infections (p=0·076). INTERPRETATION: Our data suggest that R typhi/Rickettsia spp, O tsutsugamushi, and Leptospira spp infections are important causes of CNS infections in Laos. Antibiotics, such as tetracyclines, needed for the treatment of murine typhus and scrub typhus, are not routinely advised for empirical treatment of CNS infections. These severely neglected infections represent a potentially large proportion of treatable CNS disease burden across vast endemic areas and need more attention. FUNDING: Wellcome Trust UK.

Dittrich, Sabine; Rattanavong, Sayaphet

2015-01-01

151

Resultados comparativos de la disección ganglionar en cáncer de recto con y sin tratamiento previo del tejido adiposo Comparative results of ganglion dissection in cancer of the rectum with and without prior treatment with adipose tissue  

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Full Text Available Fundamento. El propósito de este estudio es describir los resultados obtenidos mediante dos técnicas de aislamiento de ganglios linfáticos en piezas quirúrgicas de resección anterior por adenocarcinoma de recto. Material y métodos. En una serie de 30 casos consecutivos de pacientes intervenidos por adenocarcinoma de recto hemos realizado una búsqueda de ganglios de forma manual convencional y una segunda tras 24 horas en una solución desengrasante a temperatura ambiente. Resultados. En la primera búsqueda se han aislado 335 ganglios linfáticos con una media que oscila entre 6,46 y 17,58, correspondiendo los valores más bajos a los grupos que habían recibido tratamiento adyuvante previo. En la segunda inclusión, tras la acción de la solución de aclaramiento hemos encontrado nuevos ganglios (85 en un 70% de los casos, en número y tamaño sensiblemente inferior al inicial. Conclusiones. La disección ganglionar manual del tejido adiposo es un método fiable para el aislamiento de ganglios linfáticos en las piezas de resección por adenocarcinoma de recto. La búsqueda de ganglios linfáticos tras la acción de una solución de aclaramiento debe reservarse para los casos en los que no se alcanza el mínimo aconsejado en el estadiaje TNM.Background. The aim of this study is to describe the result obtained through two techniques of isolation of lymphatic lymph nodes in surgical pieces of anterior resection due to adenocarcinoma of the rectum. Material and methods. We carried out a search in a series of 30 consecutive cases of patients operated on for adenocarcinoma of the rectum for lymph nodes first in a manual conventional way and second after 24 hours in a degreasing solution at room temperature. Results. In the first search 335 lymph nodes were lymph nodes isolated, with an average that oscillated between 6.46 and 17.58, with the lower values corresponding to the groups that had received prior adjuvant treatment. In the second inclusion, following the action of the clearing solution, we found new lymph nodes (85 in some 75% of the cases, appreciably lower in number and smaller in size than the initial search. Conclusions. Manual lymph nodes dissection of the adipose tissue is a reliable method for the isolation of lymphatic lymph nodes in pieces of resection due to adenocarcinoma of the rectum. The search for lymphatic ganglions following the action of a clearing solution should be reserved for cases in which the minimum recommended in the TNM staging is not reached.

I. Amat

2003-12-01

152

Detection of Babesia Sp. EU1 and members of spotted fever group rickettsiae in ticks collected from migratory birds at Curonian Spit, North-Western Russia.  

Science.gov (United States)

To reveal the prevalence of spotted fever group (SFG) rickettsiae and Babesia sp. in Ixodes ricinus (L.) ticks from migratory birds, 236 specimens represented 8 species of Passeriformes and were collected at Curonian Spit in Kaliningrad enclave of North-Western Russia. The ticks (total 126) being detached from four bird species, Turdus philomelos, Fringilla coelebs, Parus major, and Sturnus vulgaris, were investigated by PCR using the primers Rp CS.877p/Rp CS.1258n for the detection of Rickettsia and BJ1/BN2 for Babesia spp. Babesia spp. were detected in 2 of 126 (1.6%) ticks. The partial sequence of 18S rDNA had 100% similarity to human pathogenic Babesia sp. EU1. The SFG rickettsiae were detected in 19 of 126 (15.1%) ticks collected from the above-mentioned bird species. BLAST analysis of SFG rickettsia gltA assigned sequences to human pathogenic Rickettsia helvetica (10.3%), Rickettsia monacensis (3.9%), and Rickettsia japonica (0.8%) with 98%-100% sequence similarity. The SFG rickettsiae and Babesia sp. EU1 in ticks collected from the passerines in Russia were detected for the first time. The survey indicates that migratory birds may become a reservoir for Babesia spp. and SFG rickettsiae. Future investigations need to characterize the role of birds in the epidemiology of these human pathogens in the region. PMID:20553110

Movila, Alexandru; Reye, Anna L; Dubinina, Helen V; Tolstenkov, Oleg O; Toderas, Ion; Hübschen, Judith M; Muller, Claude P; Alekseev, Andrey N

2011-01-01

153

Why Are There So Few Rickettsia conorii conorii-Infected Rhipicephalus sanguineus Ticks in the Wild?  

OpenAIRE

The bacterium Rickettsia conorii conorii is the etiological agent of Mediterranean spotted fever (MSF), which is a life-threatening infectious disease that is transmitted by Rhipicephalus sanguineus, the brown dog tick. Rh. sanguineus-R. conorii conorii relationships in the wild are still poorly understood one century after the discovery of the disease. In this study, we collected naturally infected ticks from the houses of people afflicted by MSF in Algeria. Colonies of both infected and non...

Socolovschi, Cristina; Gaudart, Jean; Bitam, Idir; Huynh, Thi Phong; Raoult, Didier; Parola, Philippe

2012-01-01

154

Coinfection with Rickettsia helvetica and Herpes simplex virus 2 in a Young Woman with Meningoencephalitis  

OpenAIRE

Herpes virus type 2 DNA was detected by PCR in the cerebrospinal fluid in a young woman presenting with headache, stiff neck and pleocytosis, and serological findings consistent with reactivation. Since she was exposed to ticks, Lyme disease and tick-borne encephalitis were excluded. Further investigation in an ongoing project, using PCR and sequencing of the amplified products, showed the presence of Rickettsia helvetica in the cerebrospinal fluid. The bacteria were also isolated in Vero cel...

Amp Hlson, Carl P.; Katarina Wallménius; Kenneth Nilsson

2011-01-01

155

Rickettsia rickettsii Infection of Cultured Human Endothelial Cells Induces Heme Oxygenase 1 Expression  

OpenAIRE

Existing evidence suggests that oxidative insults and antioxidant defense mechanisms play a critical role in the host cell response during infection of endothelial cells by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever. Heme oxygenase (HO), a rate-limiting enzyme in the pathway for heme catabolism, protects against oxidant damage in a variety of stress situations. Here, we report on the expression of the inducible and constitutive HO isozymes, HO-1 and HO-2, durin...

Rydkina, Elena; Sahni, Abha; Silverman, David J.; Sahni, Sanjeev K.

2002-01-01

156

Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction.  

OpenAIRE

Polymerase chain reaction (PCR) with nested primer pairs was used to diagnose scrub typhus and identify the Rickettsia tsutsugamushi serotype. The primer pairs used for PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen. Serotype-specific primers were used in the second PCR amplification. Five serovariants, the Gilliam, Karp, Kato, Kawasaki, and Kuroki strains of R. tsutsugamushi, were identified by nested PCR. In addition, the serotype ident...

Furuya, Y.; Yoshida, Y.; Katayama, T.; Yamamoto, S.; Kawamura, A.

1993-01-01

157

A Confirmed Case of Rickettsia parkeri Infection in a Traveler from Uruguay  

OpenAIRE

The first confirmed case of Rickettsia parkeri infection in Uruguay is reported. To date, in South America, molecularly confirmed cases of human infection have been found in Argentina and probably, Brazil. Our patient returned to Spain after a 7-day trip to Colonia Suiza (Southwestern Uruguay). He presented fever (39°C), chills, and two eschars (tache noire-like) surrounded by an indurated, erythematous halo on the inner side of the left ankle besides a maculopapular rash on the legs. After ...

Portillo, Ara?nzazu; Garci?a-garci?a, Concepcio?n; Sanz, M. Mercedes; Santiba?n?ez, Sonia; Venzal, Jose? M.; Oteo, Jose? A.

2013-01-01

158

Rickettsia-like organism infecting juvenile sea-bass Dicentrarchus labrax  

OpenAIRE

High levels of mortalities (20%) associated with abnormal swimming have been observed in farmed juveniles of seabass, Dicentrarchus labrax, in Mediterranean coast, southern France. Histopathology was obvious in the brain with nervOUS tissue necrosis and the presence of ceUs containing basophilic granules corresponding to smaU coccoid organisms. Ultrastructural examination revealed these organisms were rickettsia-like organisms morphologicaUy close to Piscirickettsia salmonis

Comps, Michel; Raymond, J. C.; Plassiart, G. N.

1996-01-01

159

Evaluation of Rickettsia japonica Pathogenesis and Reservoir Potential in Dogs by Experimental Inoculation and Epidemiologic Survey ?  

OpenAIRE

Rickettsia japonica pathogenesis and reservoir potential in dogs were evaluated by both experimental inoculation and epidemiologic survey. In the experimental inoculation study, dogs 1 and 2 were pretreated with an immunosuppressive dose of cyclosporine 14 days before inoculation and became ill after exposure to R. japonica. Dogs exhibited clinical signs, including fever, anorexia, depression, and decreased water consumption, between 36 and 96 h after inoculation, but these signs disappeared ...

Inokuma, Hisashi; Matsuda, Hironori; Sakamoto, Leo; Tagawa, Michihito; Matsumoto, Kotaro

2010-01-01

160

Molecular Typing of Isolates of Rickettsia rickettsii by Use of DNA Sequencing of Variable Intergenic Regions?  

OpenAIRE

Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, is found throughout the Americas, where it is associated with different animal reservoirs and tick vectors. No molecular typing system currently exists to allow for the robust differentiation of isolates of R. rickettsii. Analysis of eight completed genome sequences of rickettsial species revealed a high degree of sequence conservation within the coding regions of chromosomes in the genus. Intergenic regions between c...

Karpathy, Sandor E.; Dasch, Gregory A.; Eremeeva, Marina E.

2007-01-01

161

A Molecular Survey of Rickettsia felis in Fleas from Cats and Dogs in Sicily (Southern Italy)  

OpenAIRE

Rickettsia felis, the agent of flea-borne spotted fever, has a cosmopolitan distribution. Its pathogenic role in humans has been demonstrated through molecular and serologic tests in several cases. The cat flea (Ctenocephalides felis) is considered the main reservoir and the biological vector. The aim of this study was to assess the presence and occurrence of R. felis in fleas collected from dogs and cats in various sites of Palermo (Sicily). Between August and October 2012, 134 fleas were co...

Giudice, Elisabetta; Di Pietro, Simona; Alaimo, Antonio; Blanda, Valeria; Lelli, Rossella; Francaviglia, Francesco; Caracappa, Santo; Torina, Alessandra

2014-01-01

162

Occurrence of Rickettsia felis in dog and cat fleas (Ctenocephalides felis) from Italy  

OpenAIRE

Rickettsia felis is an obligate intracellular bacterium belonging to the spotted fever group, suspected to cause a murine typhus-like illness in humans, with a cosmopolitan distribution. This study was designed to estimate presence and occurrence of this pathogen in fleas collected from dogs and cats in different areas of Italy. Two species of fleas were identified, Ctenocephalides felis (80.3%) and Ctenocephalides canis (19.7%).

Capelli, Gioia; Montarsi, Fabrizio; Porcellato, Elena; Maioli, Giulia; Furnari, Carmelo; Rinaldi, Laura; Oliva, Gaetano; Otranto, Domenico

2009-01-01

163

Spotted fever group Rickettsia infecting ticks (Acari: Ixodidae in the state of Santa Catarina, Brazil  

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Full Text Available During 2006-2008, a total of 260 adult ticks were collected from domestic and wild animals in different regions of the state of Santa Catarina (SC, Brazil, including areas where human cases of Brazilian spotted fever have been reported. Collected ticks belonging to nine species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Amblyomma longirostre, Amblyomma ovale, Amblyomma tigrinum, Dermacentor nitens, Rhipicephalus microplus and Rhipicephalus sanguineus were tested by polymerase chain reaction (PCR for rickettsial infection. Overall, eight (3.1% ticks were found to be infected with Rickettsia species. After sequencing the PCR products, we determined that the sequences generated from three A. aureolatum, one A. ovale and one R. sanguineus from the municipality of Blumenau, one A. ovale from the municipality of Águas Mornas and one A. ovale from the municipality of Urussanga were identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia parkeri strain Atlantic rainforest. The sequence generated from one A. longirostre from Blumenau was 100% identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia amblyommii strain AL. Because R. parkeri strain Atlantic rainforest was recently shown to have caused two cases of human spotted fever in other states of Brazil, the role of this rickettsial agent as a possible etiological agent of spotted fever in SC is discussed.

Alessandra Pereira Medeiros

2011-12-01

164

Coexistência de linfadenite axilar tuberculosa e metástase ganglionar de carcinoma lobular de mama: relato de um caso / Coexistence of axillary tuberculous lymphadenitis and ganglionic metastasis in mammary lobular carcinoma: a case report  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Relato do caso de uma mulher com 83 anos apresentando nódulo e retração de pele na mama direita com oito meses de evolução. Ao exame físico verificou-se nódulo sólido de 5 cm, localizado no quadrante súpero-lateral de mama direita, associado a presença de retração de pele correspondente e linfonodos [...] axilares não coalescentes ipsilaterais. O resultado da mamografia evidenciou nódulo de 4 cm de diâmetro irregular no quadrante súpero-lateral da mama direita (bi-rads V). Estádio clínico: T2N1M0 (IIB). O tratamento cirúrgico incluiu mastectomia radical modificada (à Maden) com dissecção axilar níveis I, II e III. Avaliação histopatológica demonstrou a presença de carcinoma lobular infiltrativo que mediu 2,5 cm (T2), presença de linfadenite granulomatosa causada por tuberculose em linfonodos dos níveis I, II e III, associados a metástase de carcinoma lobular em um único nível linfático, nível I. Estádio patológico: pT2pN1aM0. A paciente recebeu tratamento para tuberculose ganglionar com rifampicina, isoniazida e pirazinamida por um ano. Foram solicitados receptores hormonais, os quais mostraram-se positivos, sendo feito terapia adjuvante com tamoxifeno. Durante o primeiro ano de seguimento a paciente evoluiu bem, sem sinais de recidiva local ou metástases a distância. Abstract in english Report of a case of an 83-year-old woman presenting a nodule and skin retraction in the right breast for eight months. On physical examination, a solid nodule of 5 cm was observed, located in the upper-lateral quadrant of the right breast, associated with skin retraction and ipsilateral lymph nodes. [...] Mammographic findings showed irregularly limited nodules of 4 cm in the upper-lateral quadrant of the right breast (bi-rads V). Clinical staging: T2N1M0 (IIB). Surgical treatment included a modified radical mastectomy with axillary dissection levels I, II, and III. Histopathologic evaluation demonstrated the presence of an infiltrating lobular carcinoma measuring 2.5 cm (T2), presence of granulomatous lymphadenitis caused by tuberculosis in level I, II, and III lymph nodes, associated with lobular carcinoma metastasis in a single level I lymph node. Pathologic staging: pT2pN1aM0. The treatment for the axillary tuberculous lymphadenitis was done with rifampin, isoniazid and pyrazinamide for one year. Hormone receptors were positive, and adjuvant therapy was initiated with tamoxifen. During the first year of follow-up the patient had no signal of local recurrence or distant metastases.

José Juvenal, Linhares; Eduardo Camargo, Millen; Marcelo, Antonini; Pedro César, Fagundes; Pedro Gustavo, Falcão; Joaquim Teodoro de, Araújo Neto.

2005-07-01

165

Coexistência de linfadenite axilar tuberculosa e metástase ganglionar de carcinoma lobular de mama: relato de um caso Coexistence of axillary tuberculous lymphadenitis and ganglionic metastasis in mammary lobular carcinoma: a case report  

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Full Text Available Relato do caso de uma mulher com 83 anos apresentando nódulo e retração de pele na mama direita com oito meses de evolução. Ao exame físico verificou-se nódulo sólido de 5 cm, localizado no quadrante súpero-lateral de mama direita, associado a presença de retração de pele correspondente e linfonodos axilares não coalescentes ipsilaterais. O resultado da mamografia evidenciou nódulo de 4 cm de diâmetro irregular no quadrante súpero-lateral da mama direita (bi-rads V. Estádio clínico: T2N1M0 (IIB. O tratamento cirúrgico incluiu mastectomia radical modificada (à Maden com dissecção axilar níveis I, II e III. Avaliação histopatológica demonstrou a presença de carcinoma lobular infiltrativo que mediu 2,5 cm (T2, presença de linfadenite granulomatosa causada por tuberculose em linfonodos dos níveis I, II e III, associados a metástase de carcinoma lobular em um único nível linfático, nível I. Estádio patológico: pT2pN1aM0. A paciente recebeu tratamento para tuberculose ganglionar com rifampicina, isoniazida e pirazinamida por um ano. Foram solicitados receptores hormonais, os quais mostraram-se positivos, sendo feito terapia adjuvante com tamoxifeno. Durante o primeiro ano de seguimento a paciente evoluiu bem, sem sinais de recidiva local ou metástases a distância.Report of a case of an 83-year-old woman presenting a nodule and skin retraction in the right breast for eight months. On physical examination, a solid nodule of 5 cm was observed, located in the upper-lateral quadrant of the right breast, associated with skin retraction and ipsilateral lymph nodes. Mammographic findings showed irregularly limited nodules of 4 cm in the upper-lateral quadrant of the right breast (bi-rads V. Clinical staging: T2N1M0 (IIB. Surgical treatment included a modified radical mastectomy with axillary dissection levels I, II, and III. Histopathologic evaluation demonstrated the presence of an infiltrating lobular carcinoma measuring 2.5 cm (T2, presence of granulomatous lymphadenitis caused by tuberculosis in level I, II, and III lymph nodes, associated with lobular carcinoma metastasis in a single level I lymph node. Pathologic staging: pT2pN1aM0. The treatment for the axillary tuberculous lymphadenitis was done with rifampin, isoniazid and pyrazinamide for one year. Hormone receptors were positive, and adjuvant therapy was initiated with tamoxifen. During the first year of follow-up the patient had no signal of local recurrence or distant metastases.

José Juvenal Linhares

2005-07-01

166

Isolation of "Candidatus Rickettsia andeanae" (Rickettsiales: Rickettsiaceae) in embryonic cells of naturally infected Amblyomma maculatum (Ixodida: Ixodidae).  

Science.gov (United States)

The Gulf Coast tick, Amblyomma maculatum Koch, has become increasingly important in public health for its role as a vector of the recently recognized human pathogen, Rickettsia parkeri. More recently, these ticks were also found to harbor a novel spotted fever group rickettsia, "Candidatus Rickettsia andeanae." First identified in Peru, and subsequently reported in ticks collected in the United States, Chile, and Argentina, "Ca. R. andeanae" remains largely uncharacterized, in part because of the lack of a stable isolate. Although the isolation of "Ca. R. andeanae" was recently described in DH82, Vero, and Drosophila S2 cells, its stability in these cell lines was not shown. To evaluate "Ca. R. andeanae" transmission and pathogenicity in vertebrates, as well as further describe biological characteristics of this candidate species to fulfill criteria for its establishment as a new species, availability of a stable isolate is essential. Here we describe the propagation of "Ca. R. andeanae" by using a primary culture derived from naturally infected A. maculatum embryos. Subsequent passage of the "Ca. R. andeanae" isolate to ISE6 (Ixodes scapularis embryonic) and Vero (African green monkey kidney epithelial) cell lines demonstrated limited propagation of the rickettsiae. Treatment of the infected primary cells with tetracycline resulted in cultures negative for "Ca. R. andeanae" by polymerase chain reaction and microscopy. Establishment of an isolate of "Ca. R. andeanae" will promote further investigation into the significance of this tick-associated rickettsia, including its role in spotted fever and interactions with the sympatric species, R. parkeri in A. PMID:24180118

Ferrari, F A G; Goddard, J; Moraru, G M; Smith, W E C; Varela-Stokes, A S

2013-09-01

167

Detection of anti: Rickettsia spp. antibodies in domestic chickens of extensive breeding in an endemic area for spotted fever in the state of Rio Grande do Sul, Brazil / Detecção de anticorpos anti: Rickettsia spp. em galinhas domésticas de criação extensiva em uma área endêmica para febre maculosa no estado do Rio Grande do Sul, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Este estudo teve como objetivo pesquisar anticorpos anti-Rickettsia spp. em soros de galinhas domésticas (Gallus gallus domesticus) de criação extensiva, provenientes de área considerada endêmica para febre maculosa no estado do Rio Grande do Sul. Foram coletadas 300 amostras de sangue e os soros ob [...] tidos foram testados para anticorpos anti-Rickettsia spp. pela Reação de Imunofluorescência Indireta (RIFI). A ocorrência de anticorpos anti-Rickettsia spp. observada foi de 1,33% (4/300), com títulos variando de 64 a 256 para Rickettsia rickettsii, Rickettsia parkeri e/ou Rickettsia bellii. Os resultados sugerem que essas galinhas domésticas não participam como reservatório e/ou hospedeiro amplificador na epidemiologia da febre maculosa na área endêmica. O presente estudo consiste na primeira pesquisa sorológica em Gallus gallus domesticus para rickettsia do grupo da febre maculosa no Brasil. Abstract in english The goal of this study was to investigate anti-Rickettsia spp. antibodies in sera of domestic chickens (Gallus gallus domesticus) of extensive breeding in Cerro Largo county, considered an endemic area for spotted fever in the State of Rio Grande do Sul, Brazil. Three hundred blood samples were coll [...] ected and anti-Rickettsia spp. antibodies were evaluated by indirect immunofluorescence assay (IFA) in the sera obtained. The occurrence of anti-Rickettsia spp. antibodies detected in this study was 1.33% (4/300), with endpoint titers ranging from 64 to 256 for Rickettsia rickettsiii, R. parkeri and/or R. bellii. The results suggest these domestic chickens do not participate as a reservoir and/or amplifying host in the epidemiology of spotted fever in that endemic area. The present study consists in the first serological survey in Gallus gallus domesticus to Rickettsiae-spotted fever group in Brazil.

Jonas Fernandes, Maciel; Felipe da Silva, Krawczak; Caroline Sobotyk de, Oliveira; Jonas, Moraes-Filho; Marcelo Bahia, Labruna; Sônia de Avila, Botton; Fernanda Silveira Flores, Vogel; Luis Antonio, Sangioni.

2037-20-01

168

Aproximación clínica y principales rickettsiosis transmitidas por garrapatas presentes en Latinoamérica / Clinical approach and main tick-borne rickettsiosis present in Latin America  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Las rickettsiosis transmitidas por garrapatas son infecciones potencialmente letales, que en Latinoamérica tienen carácter emergente y re-emergente. Hasta hace escasos años, la única rickettsiosis transmitida por garrapatas era causada por Rickettsia rickettsii, pero en la actualidad existen otras e [...] species como R. parkeri y R. massiliae que están provocando enfermedad en humanos en la región. Por otro lado, se están describiendo candidatos a nuevas especies de Rickettsia, que aunque no han probado su patogenicidad deben considerarse como potencialmente patógenos. Dado que el diagnóstico microbiológico puede tardar días o semanas, resulta fundamental una alta sospecha clínica y la instauración precoz de un tratamiento adecuado. En esta revisión se detalla la distribución y principales manifestaciones clínicas de las rickettsiosis transmitidas por garrapatas en Latinoamérica. Se ha incluido una sección sobre la infección por R. felis, por haberse encontrado esta especie en garrapatas, y no haberse aclarado el papel de este vector en su ciclo epidemiológico. Abstract in english Tick-borne rickettsial diseases are potentially life threatening infections that in Latin America have an emerging and reemerging character. Until few years ago, Rickettsia rickettsia was the only tick-borne rickettsia present in America; but nowadays several other species such as R. parkeri and R. [...] massiliae are causing disease in humans in the region. In addition, new species are being described; although their pathogenicity has not been confirmed they should be considered as potential pathogens. Since the microbiological diagnosis of rickettsioses can take days or weeks, a high clinical suspicion and early start of appropriate treatment are crucial. In this review the distribution and main clinical manifestations of tick-borne rickettsial diseases in Latin America are detailed. Since R. felis has been found in ticks and the role of this vector has not been clarified, we have included a section about this pathogen.

Katia, Abarca; José A, Oteo.

2014-10-01

169

Isolamento de Rickettsia em cultura de células vero  

OpenAIRE

Embora o diagnóstico da febre maculosa baseie-se em sinais e sintomas característicos, o mesmo requer confirmação laboratorial, pois existem alguns diagnósticos diferenciais possíveis como meningococcemia, leptospirose, infecção por enterovírus e febre tifóide. A confirmação laboratorial pode ser feita através da pesquisa de anticorpos específicos, possível somente alguns dias após o aparecimento da doença, através do isolamento do agente em amostras de sangue e/ou biópsia ...

Melles Heloisa Helena Barbosa; Colombo Silvia; Lemos Elba Regina Sampaio de

1999-01-01

170

High prevalence of Rickettsia typhi and Bartonella species in rats and fleas, Kisangani, Democratic Republic of the Congo.  

Science.gov (United States)

The prevalence and identity of Rickettsia and Bartonella in urban rat and flea populations were evaluated in Kisangani, Democratic Republic of the Congo (DRC) by molecular tools. An overall prevalence of 17% Bartonella species and 13% Rickettsia typhi, the agent of murine typhus, was found in the cosmopolitan rat species, Rattus rattus and Rattus norvegicus that were infested by a majority of Xenopsylla cheopis fleas. Bartonella queenslandensis, Bartonella elizabethae, and three Bartonella genotypes were identified by sequencing in rat specimens, mostly in R. rattus. Rickettsia typhi was detected in 72% of X. cheopis pools, the main vector and reservoir of this zoonotic pathogen. Co-infections were observed in rodents, suggesting a common mammalian host shared by R. typhi and Bartonella spp. Thus, both infections are endemic in DRC and the medical staffs need to be aware knowing the high prevalence of impoverished populations or immunocompromised inhabitants in this area. PMID:24445202

Laudisoit, Anne; Falay, Dadi; Amundala, Nicaise; Akaibe, Dudu; de Bellocq, Joëlle Goüy; Van Houtte, Natalie; Breno, Matteo; Verheyen, Erik; Wilschut, Liesbeth; Parola, Philippe; Raoult, Didier; Socolovschi, Cristina

2014-03-01

171

Rickettsia felis (Rickettsiales: Rickettsiaceae) in Ctenocephalides felis felis (Siphonaptera: Pulicidae) in the State of São Paulo, Brazil Rickettsia felis (Rickettsiales: Rickettsiaceae) em Ctenocephalides felis felis (Siphonaptera: Pulicidae) no estado de São Paulo  

OpenAIRE

Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene...

Horta, M. C.; Pinter, A.; Cortez, A.; Soares, R. M.; Gennari, S. M.; Schumaker, T. T. S.; Labruna, M. B.

2005-01-01

172

Prevalence of vectors of the spotted fever group Rickettsiae and murine typhus in a Bedouin town in Israel.  

Science.gov (United States)

A survey of the vectors of spotted fever group Rickettsiae and of murine typhus was carried out in Rahat, a Bedouin town in the Negev Desert, where the diseases are endemic. Houses with known cases of spotted fever group Rickettsiae or murine typhus were compared with those without reported clinical cases. A neighboring Jewish community, Lehavim, where no cases of spotted fever group Rickettsiae and murine typhus were reported in recent years, was used as a control. In the houses of patients with spotted fever group Rickettsiae in Rahat, an average of 7.4 times more ticks were found than in control houses. Out of 190 ticks isolated from sheep and goats or caught by flagging in Rahat, 90% were Rhipicephalus sanguineus (Latreille), 7.9% Rhipicephalus turanicus Pomerantzev, and 2.1% were Hyalomma sp. In the houses of patients with murine typhus, three times more rats were caught and, on the average, each rat was infested with 2.2 times more fleas than rats in the control houses. Out of 323 fleas collected from 35 Norwegian rats (Rattus norvegicus Berkenhout), 191 were Xenopsylla cheopis Rothschild and 132 Echidnophaga murina Tiraboschi. Thus, there was a six to seven times higher probability of encountering a tick or flea vector where infections had occurred than in control houses in Rahat. The percentage of rats seropositive to Rickettsia typhi was similar in study and control households (78.3 and 76.2, respectively). In the control settlement, Lehavim, only three Mus musculus L. were caught, which were not infested with ectoparasites and their sera were negative for murine typhus. Out of 10 dogs examined in this settlement, 15 R. sanguineus and eight specimens of the cat flea (Ctenocephalides felis felis Bouché) were isolated. No rats were caught in this settlement. These data indicate that there is a correlation among the density of domestic animals, their ectoparasites, and the incidence of spotted fever group Rickettsiae and murine typhus in Rahat. PMID:11372975

Mumcuoglu, K Y; Ioffe-Uspensky, I; Alkrinawi, S; Sarov, B; Manor, E; Galun, R

2001-05-01

173

The influence of temperature and pH on the growth of Rickettsia conorii in irradiated mammalian cells  

International Nuclear Information System (INIS)

The temperature range for optimum growth of Rickettsia conorii in suspension culture of gamma-irradiated L cells was 32 to 38 degC, resulting in rickettsial doubling times between 4.1 and 6.0 hrs. An asynchronous release of Rickettsia conorii from host cells was suggested by the constant increase in percent cells infected over a 36 hrs period. Rickettsial growth was optimal at neutral to slightly alkaline extracellular pH levels. A moderately acidic pH, however, resulted in an increase in doubling time from 4.1 to 7.8 hrs. (author)

174

Molecular Detection of Rickettsia felis, Bartonella henselae, and B. clarridgeiae in Fleas from Domestic Dogs and Cats in Malaysia  

OpenAIRE

The presence of Rickettsia felis, Bartonella henselae and B. clarridgeiae in 209 fleas (Ctenocephalides felis) obtained from domestic cats and dogs in several locations in Malaysia was investigated in this study. Using a polymerase chain reaction specific for the citrate synthase (gltA) and 17-kD antigenic protein (17kD) genes of rickettsiae, we detected R. felis DNA in 6 (2.9%) fleas. For detection of bartonellae, amplification of the heme-binding protein (pap31) and riboflavin synthase (rib...

Mokhtar, Aida Syafinaz; Tay, Sun Tee

2011-01-01

175

Specific amplification of Rickettsia tsutsugamushi DNA from clinical specimens by polymerase chain reaction.  

OpenAIRE

Polymerase chain reaction (PCR) was used to detect Rickettsia tsutsugamushi-specific DNA in clinical specimens. The primer pair used for PCR was designed from the nucleotide sequence of the gene encoding the 56-kDa antigen of the Gilliam strain. Theses primers led to a 78-bp fragment by amplifying the genomic DNAs from five serovariants, i.e., the Gilliam, Karp, Kato, Kawasaki, and Kuroki strains of R. tsutsugamushi, and also the DNA from blood clots of patients with scrub typhus, even at the...

Furuya, Y.; Yoshida, Y.; Katayama, T.; Kawamori, F.; Yamamoto, S.; Ohashi, N.; Tamura, A.; Kawamura, A.

1991-01-01

176

Rickettsia typhi Possesses Phospholipase A2 Enzymes that Are Involved in Infection of Host Cells  

Science.gov (United States)

The long-standing proposal that phospholipase A2 (PLA2) enzymes are involved in rickettsial infection of host cells has been given support by the recent characterization of a patatin phospholipase (Pat2) with PLA2 activity from the pathogens Rickettsia prowazekii and R. typhi. However, pat2 is not encoded in all Rickettsia genomes; yet another uncharacterized patatin (Pat1) is indeed ubiquitous. Here, evolutionary analysis of both patatins across 46 Rickettsia genomes revealed 1) pat1 and pat2 loci are syntenic across all genomes, 2) both Pat1 and Pat2 do not contain predicted Sec-dependent signal sequences, 3) pat2 has been pseudogenized multiple times in rickettsial evolution, and 4) ubiquitous pat1 forms two divergent groups (pat1A and pat1B) with strong evidence for recombination between pat1B and plasmid-encoded homologs. In light of these findings, we extended the characterization of R. typhi Pat1 and Pat2 proteins and determined their role in the infection process. As previously demonstrated for Pat2, we determined that 1) Pat1 is expressed and secreted into the host cytoplasm during R. typhi infection, 2) expression of recombinant Pat1 is cytotoxic to yeast cells, 3) recombinant Pat1 possesses PLA2 activity that requires a host cofactor, and 4) both Pat1 cytotoxicity and PLA2 activity were reduced by PLA2 inhibitors and abolished by site-directed mutagenesis of catalytic Ser/Asp residues. To ascertain the role of Pat1 and Pat2 in R. typhi infection, antibodies to both proteins were used to pretreat rickettsiae. Subsequent invasion and plaque assays both indicated a significant decrease in R. typhi infection compared to that by pre-immune IgG. Furthermore, antibody-pretreatment of R. typhi blocked/delayed phagosomal escapes. Together, these data suggest both enzymes are involved early in the infection process. Collectively, our study suggests that R. typhi utilizes two evolutionary divergent patatin phospholipases to support its intracellular life cycle, a mechanism distinguishing it from other rickettsial species. PMID:23818842

Rahman, M. Sayeedur; Gillespie, Joseph J.; Kaur, Simran Jeet; Sears, Khandra T.; Ceraul, Shane M.; Beier-Sexton, Magda; Azad, Abdu F.

2013-01-01

177

In vitro evaluation of josamycin, spiramycin, and erythromycin against Rickettsia rickettsii and R. conorii.  

OpenAIRE

The antimicrobial activities of josamycin, erythromycin, and spiramycin against Rickettsia conorii and R. rickettsii were evaluated in two tests: a dye-uptake assay and a plaque assay. The MIC of josamycin was 1 microgram/ml for both species; the MICs of erythromycin and spiramycin were 4 to 8 and 16 to 32 micrograms/ml, respectively, for both species. Only josamycin may be of clinical use in treating spotted fever rickettsiosis. It may be useful in treating pregnant women and young children.

Raoult, D.; Roussellier, P.; Tamalet, J.

1988-01-01

178

Spotted fever group rickettsiae in ticks and fleas from the Democratic Republic of the Congo.  

Science.gov (United States)

Little is known about the prevalence of spotted fever group (SFG) rickettsiae in ticks and fleas in the Democratic Republic of the Congo. In 2008, 12 Amblyomma compressum ticks were collected from 3 pangolins (Manis gigantea). Two Haemaphysalis punctaleachi ticks were collected from 2 African civets (Civettictis civetta congica), and one was collected from an antelope (Onotragus leche). A total of 111 Rhipicephalus sanguineus ticks, 23 Ctenocephalides canis fleas, 39 C. felis fleas, and 5 Trichodectes canis lice were sampled from 19 dogs. One C. canis flea was collected from a human. Six of the 12 A. compressum ticks were positive for rickettsial DNA, as determined by genus-specific qPCR. The ompA gene sequences amplified from positive samples showed 100% homology with Rickettsia africae (GenBank accession number CP001612). The detection of Ri. africae in A. compressum ticks, which are highly specialized parasites of pangolins, is consistent with our previous data showing the presence of Ri. africae in A. compressum ticks from Liberia. No other ticks contained rickettsial DNA. A total of 9 C. canis fleas (39%, 9/23) and 37 C. felis fleas (95%, 37/39) that was collected from dogs and one C. canis flea collected from a human harbored Ri. felis. PMID:23137572

Mediannikov, Oleg; Davoust, Bernard; Socolovschi, Cristina; Tshilolo, Léon; Raoult, Didier; Parola, Philippe

2012-12-01

179

A cluster of Rickettsia rickettsii infection at an animal shelter in an urban area of Brazil.  

Science.gov (United States)

SUMMARY Rickettsia rickettsii infection is being increasingly recognized as an important cause of fatal acute illness in Brazil, where this tick-borne disease is designated Brazilian spotted fever (BSF). In this study we report five fatal cases of BSF in employees of an animal shelter in an urban area in the municipality of Rio de Janeiro in southeast Brazil after a natural disaster on 11 January 2011. Four of the cases occurred from 27 January to 11 April 2011, while the fifth fatal case was identified in April 2012. Three cases were confirmed by molecular analysis and two by epidemiological linkage. An investigation of BSF was performed in the animal shelter, and blood samples were collected from 115 employees and 117 randomly selected dogs. The presence of high levels (1024-4096) of antibodies against spotted fever group rickettsiae was found in three (2·6%) employees and 114 (97·5%) dogs. These findings emphasize the need to consider BSF as a possible cause of undifferentiated febrile illness, especially dengue and leptospirosis, in patients occupationally exposed to dogs heavily infested by ticks, mainly working at kennels and animal shelters that have inadequate space for the animals housed and frequently providing an environment conducive to exposure to pathogens such as R. rickettsii. PMID:25483025

Rozental, T; Ferreira, M S; Gomes, R; Costa, C M; Barbosa, P R A; Bezerra, I O; Garcia, M H O; Oliveira E Cruz, D M; Galliez, R; Oliveira, S; Brasil, P; Rezende, T; DE Lemos, E R S

2014-12-01

180

Solubilization and reconstitution of the Rickettsia prowazekii ATP/ADP translocase.  

Science.gov (United States)

The ATP/ADP translocase protein of Rickettsia prowazekii, an obligate intracellular parasite that had been grown in the chick yolk sac, was solubilized and reconstituted into liposomes composed of Escherichia coli phospholipid by an octylglucoside dilution procedure. Proteoliposomes prepared from membranes of Renografin-purified R. prowazekii translocated ATP by an obligate exchange mechanism. Influx of extravesicular ATP required intravesicular transportable nucleotide and efflux of intravesicular ATP required transportable extravesicular nucleotide in the medium. The transport activity was insensitive to carboxyatractyloside and bongkrekic acid, inhibitors of mitochondrial ADP/ATP translocation. Proteoliposomes prepared from membranes of standard (non-Renografin-purified) R. prowazekii exhibited both an inhibitor-sensitive mitochondrial translocase activity and an inhibitor-resistant rickettsial translocase activity. Proteoliposomes prepared from uninoculated yolk sac membranes exhibited only the inhibitor-sensitive mitochondrial translocase activity. The substrate specificity of each reconstituted translocase was determined and shown to correspond with that reported for intact mitochondria or rickettsiae. Following influx of ATP the steady-state value for intravesicular labeled ATP was dependent on the concentration of intravesicular nucleotide available for exchange. PMID:2681788

Plano, G V; Winkler, H H

1989-09-01

181

Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espír [...] ito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.

KA, Oliveira; LS, Oliveira; CCA, Dias; A, Silva Jr; MR, Almeida; G, Almada; DH, Bouyer; MAM, Galvão; CL, Mafra.

2008-03-01

182

Epidemiology of Rickettsia sp. strain Atlantic rainforest in a spotted fever-endemic area of southern Brazil.  

Science.gov (United States)

The present study was performed in Vila Itoupava, an area of the state of Santa Catarina, southern Brazil, in which a tick-borne spotted fever illness has been endemic since 2003. Notably, both the etiological agent and the vector of these spotted fever cases remain unknown. During January 2011, humans, domestic dogs, and their ticks were sampled in households that are typically surrounded by highly preserved Atlantic rainforest fragments. Ticks collected from dogs were Amblyomma ovale (34% prevalence), Amblyomma aureolatum (18.9%), and Rhipicephalus sanguineus (3.8%). A total of 7.8% (6/77) A. ovale and 9.3% (4/43) A. aureolatum were infected by Rickettsia sp. strain Atlantic rainforest, a Rickettsia parkeri-like agent recently shown to cause spotted fever illness in southeastern Brazil. Overall, 67.3% (35/52) of the dogs were seroreactive to spotted fever group rickettsiae, mostly with highest endpoint titers to R. parkeri. Among humans, 46.7% (7/15) reacted serologically to rickettsiae at low to moderate endpoint titers. Because canine seroreactivity to R. parkeri was strongly associated with frequent contact with forests (the preferred habitat for A. ovale and A. aureolatum), it is concluded that sampled dogs have been infected by strain Atlantic rainforest through the parasitism of these tick species. The present study provides epidemiological evidence that the spotted fever in the study area has been caused by Rickettsia sp. strain Atlantic rainforest, transmitted to humans by either A. ovale or A. aureolatum. Further studies encompassing direct diagnostic methods on clinical specimens from patients are needed to confirm the above epidemiological evidence. PMID:25108786

Barbieri, Amalia R M; Filho, Jonas M; Nieri-Bastos, Fernanda A; Souza, Julio C; Szabó, Matias P J; Labruna, Marcelo B

2014-10-01

183

Ganglion cells in circumscribed astrocytic tumors: possible implication in classification and prognosis / Implicação da presença de células ganglionares na classificação e evolução de tumores astrocíticos circunscritos  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese INTRODUÇÃO: As neoplasias circunscritas incluem astrocitoma pilocítico (AP), xantoastrocitoma pleomórfico (XP) e ganglioglioma (GG), que compartilham diversas semelhanças, sendo o AP o de melhor prognóstico. Como as células ganglionares (CG) no GG podem ser escassas e os GGs podem recidivar ou evolu [...] ir (grau III), é fundamental o diagnóstico preciso. OBJETIVOS: Identificar CG e corpos granulares eosinofílicos (CGE) em AP e XP, avaliar sua implicação na evolução e comparar com o GG. MÉTODOS: Análise retrospectiva dos aspectos radiológicos, morfológicos e evolutivos (tempo livre de doença, recidiva e óbito) de 30 casos (14 AP, oito XP, oito GG). Cortes corados com hematoxilina e eosina (HE) foram revistos para a identificação da presença de CG neoplásicas e CGE. Estes foram imunomarcados para sinaptofisina (SIN) e neurofilamento (NF) e, em casos selecionados, para glial fibrillary acidic protein (GFAP). RESULTADOS: Seis AP foram reclassificados para GG pela presença de CG (HE ou imunomarcação). Alguns CGE, semelhantes às CG degeneradas, também imunomarcaram para SIN/NF, a maioria sendo negativa para GFAP. O tempo médio livre de doença foi de 62,16 meses. Quatro tumores recidivaram; um deles evoluiu para óbito. Todos os XP possuíam CG, sugerindo que são variantes de GG, dos quais quatro recidivaram (um óbito). O tempo médio livre de doença foi de 69 meses. O aspecto radiológico foi predominantemente cístico. CONCLUSÃO: Sugerimos que AP e XP com CG ou CGE imunopositivos para marcadores neuronais possam ser variantes de GG e alguns CGE representem CG degeneradas; entretanto, a presença de CG ganglionares parece não modificar o comportamento biológico dessas neoplasias. Abstract in english INTRODUCTION: Glial and neuroglial cell neoplasms comprise pilocytic astrocytoma (PA), pleomorphic xanthoastrocytoma (PXA) and ganglioglioma (GG), which share various similarities, though PA has better prognosis. As ganglion cells (GC) may be scarce in GG and these gangliogliomas may recur or progre [...] ss to grade III, an accurate diagnosis is essential. OBJECTIVES: The aim was to identify GC and eosinophilic granular bodies (EGB) in PA and PXA, to evaluate its effect on patient’s outcome and compare them with GG. METHODS: A retrospective analysis of radiological, morphological and follow-up aspects (disease free-survival, recurrence and death) of 30 cases (14 PA, 8 PXA, 8 GG). Hematoxylin and eosin (HE) stained sections were reviewed to identify the presence of neoplastic GC and EGB. They were immunostained for synaptophysin (SYN) and neurofilament (NF). Glial fibrillary acidic protein (GFAP) immunostaining was performed in selected cases. RESULTS: Six PA were reclassified as GG due to the presence of GC by HE or immunohistochemistry. Some EGB resembling degenerate GC were also immunostained for SYN/NF and most of them were negative for GFAP. The mean disease-free survival was 62.16 months. Four tumors recurred and one patient died. All PXA had GC, suggesting that they were variants of GG, 4 of which recurred and one patient died. Mean disease-free survival was 69 months. The radiological aspect was predominantly cystic. CONCLUSION: We propose that PA and PXA with GC or with EGB immunopositive for neuronal markers could be variants of GG, and some EGB may represent degenerate GC. However, the presence of GC does not seem to modify the biological behavior of these neoplasms.

Veronica Goulart, Moreira; Nathalie Henriques Silva, Canedo; Leila Maria Cardão, Chimelli.

2013-06-01

184

Antibodies to Rickettsia rickettsii, Rickettsia typhi, Coxiella burnetii, Bartonella henselae, Bartonella quintana, and Ehrlichia chaffeensis among healthy population in Minas Gerais, Brazil  

Directory of Open Access Journals (Sweden)

Full Text Available Rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in South America particularly in Brazil where few epidemiological reports have been published. We describe a serosurvey for Rickettsia rickettsii, R. typhi, Coxiella burnetii, Bartonella henselae, B. quintana, and Ehrlichia chaffeensis in 437 healthy people from a Brazilian rural community. The serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 was used. The seroprevalence rates for R. rickettsii, R. typhi, C. burnetii, B. henselae, B. quintana, and E. chaffeensis were respectively 1.6% (7 samples; 1.1% (5 samples; 3.9% (17 samples; 13.7% (60 samples; 12.8% (56 samples, and 10.5% (46 samples. Frequent multiple/cross-reactivity was observed in this study. Age over 40 years old, urban profession, and rural residence were significantly associated with some but not all infections rate. Low seropositivity rates for R. rickettsii, R. typhi, and C. burnetii contrasted with higher rates of seropositivity for B. quintana, B. henselae, and E. chaffeensis. These results show that all tested rickettsial species or antigenically closely related possible exist in this particular region.

Paulo Sérgio Gonçalves da Costa

2005-12-01

185

Antibodies to Rickettsia rickettsii, Rickettsia typhi, Coxiella burnetii, Bartonella henselae, Bartonella quintana, and Ehrlichia chaffeensis among healthy population in Minas Gerais, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in South America particularly in Brazil where few epidemiological reports have been published. We describe a serosurvey for Rickettsia rickettsii, R. typhi, Coxiella burnetii, Bartonella henselae, B. [...] quintana, and Ehrlichia chaffeensis in 437 healthy people from a Brazilian rural community. The serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 was used. The seroprevalence rates for R. rickettsii, R. typhi, C. burnetii, B. henselae, B. quintana, and E. chaffeensis were respectively 1.6% (7 samples); 1.1% (5 samples); 3.9% (17 samples); 13.7% (60 samples); 12.8% (56 samples), and 10.5% (46 samples). Frequent multiple/cross-reactivity was observed in this study. Age over 40 years old, urban profession, and rural residence were significantly associated with some but not all infections rate. Low seropositivity rates for R. rickettsii, R. typhi, and C. burnetii contrasted with higher rates of seropositivity for B. quintana, B. henselae, and E. chaffeensis. These results show that all tested rickettsial species or antigenically closely related possible exist in this particular region.

Paulo Sérgio Gonçalves da, Costa; Marcos Emilio, Brigatte; Dirceu Bartolomeu, Greco.

2005-12-01

186

Hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to Ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR.  

OpenAIRE

Ixodid ticks were collected from Connecticut, Massachusetts, Missouri, Pennsylvania, Rhode Island, and British Columbia (Canada) during 1991 to 1994 to determine the prevalence of infection with hemocytic (blood cell), rickettsia-like organisms. Hemolymph obtained from these ticks was analyzed by direct and indirect fluorescent antibody (FA) staining methods with dog, horse, or human sera containing antibodies to Ehrlichia canis, Ehrlichia equi, or Rickettsia rickettsii. Of the 693 nymphal an...

Magnarelli, L. A.; Stafford, K. C.; Mather, T. N.; Yeh, M. T.; Horn, K. D.; Dumler, J. S.

1995-01-01

187

Serosurvey of antibodies against spotted fever group Rickettsia spp. in horse farms in Northern Paraná, Brazil Soroprevalência de anticorpos contra Rickettsia spp. do grupo febre maculosa em equinos de haras no Norte do Paraná, Brasil  

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Full Text Available Brazilian spotted fever (BSF is an emerging disease most likely caused by Rickettsia rickettsii. The objective of the present study was to estimate the seroprevalence of BSF rickettsia infections in equines from six horse farms located in Londrina County, Paraná, Southern Brazil. Six owners of horse farms situated in Cambé, Santa Fé, Guaraci and Londrina municipalities participated in the study. All farms were located in areas where BSF has not been reported. A total of 273 horses were sampled and their sera were tested by indirect Immunofluorescence assay (IFA using R. rickettsii and R. parkeri antigens. Titers equal to and greater than 64 were considered positive. Of 273 sera tested, 15 (5.5% reacted to R. rickettsii and 5 (1.8% to R. parkeri. Five out of the six farms studied revealed seropositive animals and seropositivity rate ranged from 0 to 13%. The titers ranged from 64 to 512, and four samples had a titer of 512. Nine animals reacted to R. rickettsii with titers four-fold higher than those for R. parkeri. These results suggest that horses in Northern Paraná may have been exposed to rickettsiae identical or closely related to R. rickettsii.Febre Maculosa Brasileira (FMB é uma doença emergente, sendo Rickettsia rickettsii o seu principal agente etiológico. O objetivo deste estudo foi determinar a soroprevalência de rickettsia do grupo da febre maculosa em equinos de seis haras localizados nos municípios de Cambé, Santa Fé, Guaraci e Londrina. As propriedades eram localizadas na região Norte do Paraná onde casos de FMB ainda não foram diagnosticados. Foram colhidas amostras de sangue de 273 equinos, e os soros foram testados pela RIFI, usando R. rickettsii e R. parkeri como antígenos, considerando-se como positivos títulos >64. Entre 273 soros, 15 (5,5% reagiram contra R. rickettsii e 5 (1,8% para R. parkeri. Cinco de seis haras estudados tinham animais reativos, e a taxa de sororreatividade variou de 0 a 13%. Os títulos variaram de 64 para 512, e três amostras apresentaram título de 512. Nove animais reagiram para R. rickettsii com títulos quatro vezes maiores que para R. parkeri. Esses resultados sugerem que equinos no Norte do Estado do Paraná, Brasil, podem ter sido expostos a uma rickettsia idêntica ou muito próxima a R. rickettsii.

Katia Tamekuni

2010-12-01

188

Serosurvey of antibodies against spotted fever group Rickettsia spp. in horse farms in Northern Paraná, Brazil / Soroprevalência de anticorpos contra Rickettsia spp. do grupo febre maculosa em equinos de haras no Norte do Paraná, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Febre Maculosa Brasileira (FMB) é uma doença emergente, sendo Rickettsia rickettsii o seu principal agente etiológico. O objetivo deste estudo foi determinar a soroprevalência de rickettsia do grupo da febre maculosa em equinos de seis haras localizados nos municípios de Cambé, Santa Fé, Guaraci e L [...] ondrina. As propriedades eram localizadas na região Norte do Paraná onde casos de FMB ainda não foram diagnosticados. Foram colhidas amostras de sangue de 273 equinos, e os soros foram testados pela RIFI, usando R. rickettsii e R. parkeri como antígenos, considerando-se como positivos títulos >64. Entre 273 soros, 15 (5,5%) reagiram contra R. rickettsii e 5 (1,8%) para R. parkeri. Cinco de seis haras estudados tinham animais reativos, e a taxa de sororreatividade variou de 0 a 13%. Os títulos variaram de 64 para 512, e três amostras apresentaram título de 512. Nove animais reagiram para R. rickettsii com títulos quatro vezes maiores que para R. parkeri. Esses resultados sugerem que equinos no Norte do Estado do Paraná, Brasil, podem ter sido expostos a uma rickettsia idêntica ou muito próxima a R. rickettsii. Abstract in english Brazilian spotted fever (BSF) is an emerging disease most likely caused by Rickettsia rickettsii. The objective of the present study was to estimate the seroprevalence of BSF rickettsia infections in equines from six horse farms located in Londrina County, Paraná, Southern Brazil. Six owners of hors [...] e farms situated in Cambé, Santa Fé, Guaraci and Londrina municipalities participated in the study. All farms were located in areas where BSF has not been reported. A total of 273 horses were sampled and their sera were tested by indirect Immunofluorescence assay (IFA) using R. rickettsii and R. parkeri antigens. Titers equal to and greater than 64 were considered positive. Of 273 sera tested, 15 (5.5%) reacted to R. rickettsii and 5 (1.8%) to R. parkeri. Five out of the six farms studied revealed seropositive animals and seropositivity rate ranged from 0 to 13%. The titers ranged from 64 to 512, and four samples had a titer of 512. Nine animals reacted to R. rickettsii with titers four-fold higher than those for R. parkeri. These results suggest that horses in Northern Paraná may have been exposed to rickettsiae identical or closely related to R. rickettsii.

Katia, Tamekuni; Roberta dos Santos, Toledo; Mauro de Freitas, Silva Filho; Valeska Bender, Haydu; Richard Campos, Pacheco; José Henrique, Cavicchioli; Marcelo Bahia, Labruna; John Stephen, Dumler; Odilon, Vidotto.

2010-12-01

189

Serosurvey for tick-borne diseases in dogs from the Eastern Amazon, Brazil / Pesquisa Sorologica por doencas transmitidas por carrapatos em caes da Amazonia oriental, Brasil  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese Ehrliquiose canina e babesiose canina são as doenças parasitárias transmitidas por carrapatos de maior prevalência em cães do Brasil. Poucos estudos pesquisaram doenças transmitidas por carrapatos na região da Amazônia brasileira. Um total de 129 amostras de sangue foram colhidas de cães da Amazônia [...] oriental brasileira. Setenta e dois cães eram de áreas rurais de 19 municípios do Estado do Pará, e 57 amostras foram colhidas de cães errantes vadios da área urbana do município de Santarém-PA. As amostras de soro foram submetidas ao ensaio de imunofluorescência indireta, com antígenos de Babesia canis vogeli, Ehrlichia canis, e seis espécies de Rickettsia. A frequência de cães com anticorpos anti-B. canis vogeli, anti-E. canis, e anti-Rickettsia spp. foi de 42,6%, 16,2% e 31,7%, respectivamente. Anticorpos anti-B. canis vogeli foram detectados em 59,6% dos cães urbanos, e em 29,1% dos cães rurais (P Abstract in english Canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in Brazilian dogs. Few studies have focused attention in surveying tick-borne diseases in the Brazilian Amazon region. A total of 129 blood samples were collected from dogs living in the Brazilian eastern Amazon. Seventy-t [...] wo samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from Santarém municipality were collected. Serum samples were submitted to Indirect Immunofluorescence Assay (IFA) with antigens of Babesia canis vogeli, Ehrlichia canis, and six Rickettsia species. The frequency of dogs containing anti-B. canis vogeli, anti-E. canis, and anti-Rickettsia spp. antibodies was 42.6%, 16.2%, and 31.7%, respectively. Anti-B. canis vogeli antibodies were detected in 59.6% of the urban dogs, and in 29.1% of the rural dogs (P

Mariana Granziera, Spolidorio; Antonio Humberto Hamad, Minervino; Samantha Yuri Oshiro Branco, Valadas; Herbert Sousa, Soares; Kedson Alessandri Lobo, Neves; Marcelo Bahia, Labruna; Mucio Flavio Barbosa, Ribeiro; Solange Maria, Gennari.

2013-06-25

190

High prevalence of Rickettsia africae variants in Amblyomma variegatum ticks from domestic mammals in rural western Kenya: implications for human health.  

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Tick-borne spotted fever group (SFG) rickettsioses are emerging human diseases caused by obligate intracellular Gram-negative bacteria of the genus Rickettsia. Despite being important causes of systemic febrile illnesses in travelers returning from sub-Saharan Africa, little is known about the reservoir hosts of these pathogens. We conducted surveys for rickettsiae in domestic animals and ticks in a rural setting in western Kenya. Of the 100 serum specimens tested from each species of domestic ruminant 43% of goats, 23% of sheep, and 1% of cattle had immunoglobulin G (IgG) antibodies to the SFG rickettsiae. None of these sera were positive for IgG against typhus group rickettsiae. We detected Rickettsia africae-genotype DNA in 92.6% of adult Amblyomma variegatum ticks collected from domestic ruminants, but found no evidence of the pathogen in blood specimens from cattle, goats, or sheep. Sequencing of a subset of 21 rickettsia-positive ticks revealed R. africae variants in 95.2% (20/21) of ticks tested. Our findings show a high prevalence of R. africae variants in A. variegatum ticks in western Kenya, which may represent a low disease risk for humans. This may provide a possible explanation for the lack of African tick-bite fever cases among febrile patients in Kenya. PMID:25325312

Maina, Alice N; Jiang, Ju; Omulo, Sylvia A; Cutler, Sally J; Ade, Fredrick; Ogola, Eric; Feikin, Daniel R; Njenga, M Kariuki; Cleaveland, Sarah; Mpoke, Solomon; Ng'ang'a, Zipporah; Breiman, Robert F; Knobel, Darryn L; Richards, Allen L

2014-10-01

191

Some ultrastructural effects of persistent infections by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (Vero) cells.  

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Mouse fibroblasts (L-929) and Vero (green monkey kidney) cells were infected with the rickettsia Coxiella burnetti, and persistent infections developed and were studied over a 6- to 10-month period. Ultrastructural comparisons were made between the two infected cell types, and both were tested cytochemically for the presence of acid phosphatase, a marker enzyme of lysozymes. Rickettsiae were always observed within vacuoles, and some infected L cells showed flattened endoplasmic reticulum as compared with uninfected cells. Rickettsiae in Vero cells were most often seen in vacuoles containing whorls of membranes ("myelin configurations") which were also seen in uninfected cells. Rickettsiae in Vero cells were pleomorphic, with acid phosphatase reaction product in their periplasmic space. This suggests either rickettsial degradation by lysosomal enzymes which penetrated the cell envelope or a penetration after the rickettsiae were dead. Vacuoles of infected Vero cells showed much more reaction product than that in infected L cells, and most rickettsiae in L cells had a normal appearance and showed no reaction product in their periplasmic space. Images PMID:99368

Burton, P R; Stueckemann, J; Welsh, R M; Paretsky, D

1978-01-01

192

Candidatus Rickettsia andeanae, a spotted fever group agent infecting Amblyomma parvum ticks in two Brazilian biomes  

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Full Text Available SciELO Brazil | Language: English Abstract in english Adult ticks of the species Amblyomma parvum were collected from the vegetation in the Pantanal biome (state of Mato Grosso do Sul) and from horses in the Cerrado biome (state of Piauí) in Brazil. The ticks were individually tested for rickettsial infection via polymerase chain reaction (PCR) targeti [...] ng three rickettsial genes, gltA, ompA and ompB. Overall, 63.5% (40/63) and 66.7% (2/3) of A. parvum ticks from Pantanal and Cerrado, respectively, contained rickettsial DNA, which were all confirmed by DNA sequencing to be 100% identical to the corresponding fragments of the gltA, ompA and ompB genes of Candidatus Rickettsia andeanae. This report is the first to describe Ca. R. andeanae in Brazil.

Fernanda Aparecida, Nieri-Bastos; Marcos Gomes, Lopes; Paulo Henrique Duarte, Cançado; Giselle Ayres Razera, Rossa; João Luiz Horácio, Faccini; Solange Maria, Gennari; Marcelo Bahia, Labruna.

2014-04-01

193

Feeding Period Required by Amblyomma aureolatum Ticks for Transmission of Rickettsia rickettsii to Vertebrate Hosts  

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Rocky Mountain spotted fever is endemic to the São Paulo metropolitan area, Brazil, where the etiologic agent, Rickettsia rickettsii, is transmitted to humans by adult Amblyomma aureolatum ticks. We determined the minimal feeding period required by A. aureolatum nymphs and adults to transmit R. rickettsii to guinea pigs. Unfed nymphs and unfed adult ticks had to be attached to the host for >10 hours to transmit R. rickettsii. In contrast, fed ticks needed a minimum of 10 minutes of attachment to transmit R. rickettsii to hosts. Most confirmed infections of Rocky Mountain spotted fever in humans in the São Paulo metropolitan area have been associated with contact with domestic dogs, the main host of A. aureolatum adult ticks. The typical expectation that transmission of tickborne bacteria to humans as well as to dogs requires ?2 hours of tick attachment may discourage persons from immediately removing them and result in transmission of this lethal bacterium. PMID:25148391

Saraiva, Danilo G.; Soares, Herbert S.; Soares, João Fábio

2014-01-01

194

Rickettsia felis in Ctenocephalides felis felis from five geographic regions of Brazil.  

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This study evaluated rickettsial infection in 701 Ctenocephalides felis felis fleas that were collected from dogs and cats in 31 municipalities, encompassing all regions and major biomes of Brazil. A total of 268 (38.2%) fleas from 30 municipalities were polymerase chain reaction (PCR) positive for the rickettsial gltA gene. The PCR products from 44 fleas, consisting of at least 1 PCR-positive flea from each of 30 municipalities, generated DNA sequences identical to Rickettsia felis. Rickettsial prevalence was highly variable among 30 municipalities, with values ranging from 2.9% to 100%. Significantly higher infection rates by R. felis were associated with the Pampa biome (southern Brazil), and the temperate climate that prevails in southern Brazil. In contrast, lowest R. felis-infection rates were significantly associated with the Caatinga biome, and its semiarid climate. Further studies should evaluate the effect of temperature and moisture on the R. felis infection in Ctenocephalides fleas world widely. PMID:24778194

Horta, Mauricio C; Ogrzewalska, Maria; Azevedo, Milka C; Costa, Francisco B; Ferreira, Fernando; Labruna, Marcelo B

2014-07-01

195

Why Are There So Few Rickettsia conorii conorii-Infected Rhipicephalus sanguineus Ticks in the Wild?  

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Background Rickettsia conorii conorii is the etiological agent of Mediterranean spotted fever, which is transmitted by the brown dog tick, Rhipicephalus sanguineus. The relationship between the Rickettsia and its tick vector are still poorly understood one century after the first description of this disease. Methodology/Principal Findings An entomological survey was organized in Algeria to collect ticks from the houses of patients with spotted fever signs. Colonies of R. conorii conorii-infected and non-infected ticks were established under laboratory conditions. Gimenez staining and electron microscopy on the ovaries of infected ticks indicated heavy rickettsial infection. The transovarial transmission of R. conorii conorii in naturally infected Rh. sanguineus ticks was 100% at eleven generations, and the filial infection rate was up to 99% according to molecular analyses. No differences in life cycle duration were observed between infected and non-infected ticks held at 25°C, but the average weight of engorged females and eggs was significantly lower in infected ticks than in non-infected ticks. The eggs, larvae and unfed nymphs of infected and non-infected ticks could not tolerate low (4°C) or high (37°C) temperatures or long starvation periods. R. conorii conorii-infected engorged nymphs that were exposed to a low or high temperature for one month experienced higher mortality when they were transferred to 25°C than non-infected ticks after similar exposure. High mortality was observed in infected adults that were maintained for one month at a low or high temperature after tick-feeding on rabbits. Conclusion/Significance These preliminary results suggest that infected quiescent ticks may not survive the winter and may help explain the low prevalence of infected Rh. sanguineus in nature. Further investigations on the influence of extrinsic factors on diapaused R. conorii-infected and non-infected ticks are required. PMID:22724035

Socolovschi, Cristina; Gaudart, Jean; Bitam, Idir; Huynh, Thi Phong; Raoult, Didier; Parola, Philippe

2012-01-01

196

Ultrastructure of a haplosporidian containing Rickettsiae, associated with mortalities among cultured paua Haliotis iris.  

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Uninucleate and multinucleate stages of a protozoan parasite are described from cultured abalone Haliotis iris Martyn, 1784 in New Zealand. The parasite is identified as a haplosporidian by the occurrence of multinucleate plasmodia, mitochondria with tubular cristae, lipid droplets, anastomosing endoplasmic reticulum (aER), multivesicular bodies (MVBs), haplosporogenesis by the production of haplosporosome-like bodies from nuclear membrane-bound Golgi, and their maturation to haplosporosomes. Coated pits occurred in the plasma membrane and coated vesicles were scattered in the cytoplasm, particularly in association with the Golgi face away from the nucleus, and aER. It is concluded that the outward face of the Golgi may be the trans face, and that aER is the trans-Golgi network. Coated pits and bristle-coated vesicles are reported from a haplosporidian for the first time. The vesicles in the MVBs resembled the cores and inner membranes of haplosporosomes, without the outer layer. The possible inter-relationships of these features are discussed. The abalone parasite differs from previously described haplosporidians in the apparent absence of a persistent mitotic spindle, and the presence of intracytoplasmic coccoid to rod-shaped bacteria resembling Rickettsiales-like prokaryotes. Phylogenetic analysis of the 16S rRNA gene sequence of the Rickettsiales-like prokaryotes indicated that these organisms belong to the Rickettsia cluster. The prokaryotes have a high (7%) sequence divergence from known Rickettsieae, with Rickettsia sp. and R. massiliae being the closest relatives. The lack of non-molecular evidence prevents us from proposing a new rickettsial genus at this time. PMID:12113307

Hine, P M; Wakefield, S; Diggles, B K; Webb, V L; Maas, E W

2002-06-01

197

Genomic Diversification in Strains of Rickettsia felis Isolated from Different Arthropods.  

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Rickettsia felis (Alphaproteobacteria: Rickettsiales) is the causative agent of an emerging flea-borne rickettsiosis with worldwide occurrence. Originally described from the cat flea, Ctenocephalides felis, recent reports have identified R. felis from other flea species, as well as other insects and ticks. This diverse host range for R. felis may indicate an underlying genetic variability associated with host-specific strains. Accordingly, to determine a potential genetic basis for host specialization, we sequenced the genome of R. felis str. LSU-Lb, which is an obligate mutualist of the parthenogenic booklouse Liposcelis bostrychophila (Insecta: Psocoptera). We also sequenced the genome of R. felis str. LSU, the second genome sequence for cat flea-associated strains (cf. R. felis str. URRWXCal2), which are presumably facultative parasites of fleas. Phylogenomics analysis revealed R. felis str. LSU-Lb diverged from the flea-associated strains. Unexpectedly, R. felis str. LSU was found to be divergent from R. felis str. URRWXCal2, despite sharing similar hosts. Although all three R. felis genomes contain the pRF plasmid, R. felis str. LSU-Lb carries an additional unique plasmid, pLbaR (plasmid of L. bostrychophila associated Rickettsia), nearly half of which encodes a unique 23-gene integrative conjugative element. Remarkably, pLbaR also encodes a repeats-in-toxin-like type I secretion system and associated toxin, heretofore unknown from other Rickettsiales genomes, which likely originated from lateral gene transfer with another obligate intracellular parasite of arthropods, Cardinium (Bacteroidetes). Collectively, our study reveals unexpected genomic diversity across three R. felis strains and identifies several diversifying factors that differentiate facultative parasites of fleas from obligate mutualists of booklice. PMID:25477419

Gillespie, Joseph J; Driscoll, Timothy P; Verhoeve, Victoria I; Utsuki, Tadanobu; Husseneder, Claudia; Chouljenko, Vladimir N; Azad, Abdu F; Macaluso, Kevin R

2014-01-01

198

Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted fever in the city of Rio de Janeiro, Brazil  

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Full Text Available A lethal case of Brazilian spotted fever (BSF is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA for spotted fever group rickettsia (SFGR was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR and the nucleotide sequencing of a fragment of the ompA gene showed 100% homology to Rickettsia rickettsii. BSF has not been reported in the city of Rio de Janeiro in the last three decades, and the present description should alert the clinicians to its presence in urban Rio de Janeiro, and to the differential diagnosis with dengue fever, gastroenteritis, leptospirosis and bacterial septic shock, among others.

Cristiane Lamas

2008-04-01

199

Serosurvey for tick-borne diseases in dogs from the Eastern Amazon, Brazil Pesquisa Sorológica por doenças transmitidas por carrapatos em cães da Amazônia oriental, Brasil  

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Full Text Available Canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in Brazilian dogs. Few studies have focused attention in surveying tick-borne diseases in the Brazilian Amazon region. A total of 129 blood samples were collected from dogs living in the Brazilian eastern Amazon. Seventy-two samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from Santarém municipality were collected. Serum samples were submitted to Indirect Immunofluorescence Assay (IFA with antigens of Babesia canis vogeli, Ehrlichia canis, and six Rickettsia species. The frequency of dogs containing anti-B. canis vogeli, anti-E. canis, and anti-Rickettsia spp. antibodies was 42.6%, 16.2%, and 31.7%, respectively. Anti-B. canis vogeli antibodies were detected in 59.6% of the urban dogs, and in 29.1% of the rural dogs (P Ehrliquiose canina e babesiose canina são as doenças parasitárias transmitidas por carrapatos de maior prevalência em cães do Brasil. Poucos estudos pesquisaram doenças transmitidas por carrapatos na região da Amazônia brasileira. Um total de 129 amostras de sangue foram colhidas de cães da Amazônia oriental brasileira. Setenta e dois cães eram de áreas rurais de 19 municípios do Estado do Pará, e 57 amostras foram colhidas de cães errantes vadios da área urbana do município de Santarém-PA. As amostras de soro foram submetidas ao ensaio de imunofluorescência indireta, com antígenos de Babesia canis vogeli, Ehrlichia canis, e seis espécies de Rickettsia. A frequência de cães com anticorpos anti-B. canis vogeli, anti-E. canis, e anti-Rickettsia spp. foi de 42,6%, 16,2% e 31,7%, respectivamente. Anticorpos anti-B. canis vogeli foram detectados em 59,6% dos cães urbanos, e em 29,1% dos cães rurais (P < 0.05. Para E. canis, a soroprevalência foi parecida entre os cães urbanos (15,7% e rurais (16,6%. Para Rickettsia spp., cães rurais apresentaram prevalência (P < 0.05 significativamente maior (40,3% do que os cães urbanos (21,1%. Esse primeiro estudo sobre agentes transmitidos por carrapatos entre cães da Amazônia oriental brasileira indica que estes animais estão expostos a vários agentes. Estes incluem Babesia principalmente na área urbana, Riquétsias do grupo da Febre Maculosa principalmente nas áreas rurais, e Erliquia em cães de ambas as áreas, rural e urbana.

Mariana Granziera Spolidorio

200

Lawsonia intracellularis Contains a Gene Encoding a Functional Rickettsia-Like ATP/ADP Translocase for Host Exploitation?  

OpenAIRE

ATP/ADP translocases are a hallmark of obligate intracellular pathogens related to chlamydiae and rickettsiae. These proteins catalyze the highly specific exchange of bacterial ADP against host ATP and thus allow bacteria to exploit their hosts' energy pool, a process also referred to as energy parasitism. The genome sequence of the obligate intracellular pathogen Lawsonia intracellularis (Deltaproteobacteria), responsible for one of the most economically important diseases in the swine indus...

Schmitz-esser, Stephan; Haferkamp, Ilka; Knab, Silvia; Penz, Thomas; Ast, Michelle; Kohl, Christian; Wagner, Michael; Horn, Matthias

2008-01-01

201

First molecular evidence of Anaplasma ovis and Rickettsia spp. in keds (Diptera: Hippoboscidae) of sheep and wild ruminants.  

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To evaluate the presence of rickettsial agents in hippoboscid flies with molecular methods, 81 sheep keds (Melophagus ovinus) were collected from 23 sheep, 144 deer keds (Lipoptena cervi) were caught in the environment, and a further 463 and 59 individuals of the latter species were obtained from fresh carcasses of 29 red deer and 17 roe deer, respectively. DNA was extracted individually or in pools. Anaplasma ovis was demonstrated in all examined sheep keds, and from one pool of free-living deer keds. Rickettsia helvetica or other, unidentified rickettsiae were also present in one pool of sheep keds, and in four pools of deer keds from both red deer and roe deer. This is the first account of polymerase chain reaction positivity of hippoboscid flies for A. ovis and rickettsiae. These results raise the possibility that-apart from cattle and roe deer as already reported-sheep and red deer might also play a reservoir role in the epidemiology of rickettsioses. PMID:21923269

Hornok, Sándor; de la Fuente, José; Biró, Nóra; Fernández de Mera, Isabel G; Meli, Marina L; Elek, Vilmos; Gönczi, Eniko; Meili, Theres; Tánczos, Balázs; Farkas, Róbert; Lutz, Hans; Hofmann-Lehmann, Regina

2011-10-01

202

Molecular characterization of Rickettsia massiliae and Anaplasma platys infecting Rhipicephalus sanguineus ticks and domestic dogs, Buenos Aires (Argentina).  

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Rickettsioses, ehrlichioses and anaplasmoses are emerging diseases that are mainly transmitted by arthropods and that affect humans and animals. The aim of the present study was to use molecular techniques to detect and characterize those pathogens in dogs and ticks from Buenos Aires city. We studied 207 Rhipicephalus sanguineus ticks and 52 canine blood samples from poor neighborhoods of Buenos Aires city. The samples were molecularly screened for the genera Rickettsia, Ehrlichia, and Anaplasma by PCR and sequencing. DNA of Rickettsia massiliae (3.4%) and Anaplasma platys (13.5%) was detected in ticks and blood samples, respectively. For characterization, the positive samples were subjected to amplification of a fragment of the 190-kDa outer membrane protein gene (spotted fever group rickettsiae) and a fragment of the groESL gene (specific for A. platys). A phylogenetic tree was constructed using the neighbor-joining method, revealing that the sequences were closely related to those of strains from other geographic regions. The results indicate that human and animal pathogens are abundant in dogs and their ticks in Buenos Aires city and portray the potentially high risk of human exposure to infection with these agents, especially in poor neighborhoods, where there is close contact with animals in an environment of poor health conditions. PMID:24907186

Cicuttin, Gabriel L; Brambati, Diego F; Rodríguez Eugui, Juan I; Lebrero, Cecilia González; De Salvo, María N; Beltrán, Fernando J; Gury Dohmen, Federico E; Jado, Isabel; Anda, Pedro

2014-09-01

203

First record of Ixodes arboricola (Ixodida: Ixodidae) from Turkey with presence of Candidatus Rickettsia vini (Rickettsiales: Rickettsiaceae).  

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Birds are the specific hosts of many tick species and may contribute to the dissemination of ticks and tick-borne pathogens throughout the world. Determination of ticks infesting birds and their pathogens are important for the detection of natural foci of human pathogens. Unfortunately, there is very limited information about the occurrence of ticks on birds and associated pathogens in Turkey. We performed a tick survey on three passerine bird species; Parus major, Sylvia atricapilla, and Turdus merula. Ticks collected from these birds were identified to species and tested for the presence of Borrelia, Ehrlichia, and Rickettsia species. Ixodes arboricola Schulze & Schlottke, Ixodes frontalis Panzer, and Ixodes ricinus L. were found on the birds. This is the first study reporting the presence of I. frontalis and I. arboricola on S. atricapilla and P. major, respectively, in Turkey. In addition, the results of polymerase chain reaction (PCR) with primers specific for gltA and ompA genes and DNA sequence analysis of positive PCR products indicated the presence of Candidatus Rickettsia vini in I. arboricola ticks. In conclusion, this is the first record of both I. arboricola and Candidatus Rickettsia vini in Turkey. Therefore, future studies needed to be conducted on the ticks infesting birds and their pathogens to elucidate the role of birds in the dispersal of tick species and tick-borne pathogens in Turkey. PMID:25118420

Keskin, Adem; Koprulu, Tugba Kul; Bursali, Ahmet; Ozsemir, Arif Cemal; Yavuz, Kiraz Erciyas; Tekin, Saban

2014-07-01

204

Rickettsia felis (Rickettsiales: Rickettsiaceae) in Ctenocephalides felis felis (Siphonaptera: Pulicidae) in the State of São Paulo, Brazil / Rickettsia felis (Rickettsiales: Rickettsiaceae) em Ctenocephalides felis felis (Siphonaptera: Pulicidae) no estado de São Paulo  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese Amostras de 10 e 14 pulgas Ctenocephalides felis felis foram coletadas de cães nos municípios de Pedreira e Mogi das Cruzes, respectivamente, no estado de São Paulo, para pesquisa de Rickettsia spp. As pulgas foram individualmente submetidas à reação em cadeia pela polimerase, tendo como alvo os gen [...] es 17-kDa e 190-kDa (OmpA) de Rickettsia, sendo esse último específico para o GFM. Nove pulgas de Pedreira (90%) e quatro pulgas de Mogi das Cruzes (28%) foram positivas para o gene 17-kDa, e oito pulgas de Pedreira (80%) e quatro de Mogi das Cruzes (28%) foram positivas para o gene 190-kDa. As seqüências de nucleotídeos do gene 190-kDa de uma pulga de Pedreira e de uma pulga de Mogi das Cruzes foram 100% idênticas; quando comparadas com dados existentes no GenBank, foram 100% idênticas com a seqüência parcial do gene 190-kDa de Rickettsia felis. Esse foi o primeiro relato de sua ocorrência no estado de São Paulo. Abstract in english Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the [...] 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene, and eight fleas from Pedreira (80%) and four from Mogi das Cruzes (28%) were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to the GenBank Data, they were 100% identical to the 190-kDa sequence of R. felis. This was the first report of its occurrence in the State of São Paulo.

M.C., Horta; A., Pinter; A., Cortez; R.M., Soares; S.M., Gennari; T.T.S., Schumaker; M.B., Labruna.

2005-06-01

205

Índice ganglionar y número de linfonodos metastásicos como factores pronósticos en cáncer de colon / Lymph node index as a prognostic factor for survival in stage III colon cancer  

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Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: El índice ganglionar (IG) se ha propuesto como un factor pronóstico mejor que el número de LN positivos en cáncer de colon estadio III. El objetivo es comparar estos factores en una serie clínica. Pacientes y Método: Se incluyen todos los pacientes estadio III resecados con intención c [...] urativa (R0). Se compara la sobrevida según el número de LN positivos y el IG mediante el análisis de las curvas ROC. Resultados: Se trata de 115 pacientes con un promedio de edad de 67,9 años (extremos 25-91), el 63,4% mujeres. El compromiso en profundidad del tumor fue T2 en 3 casos, T3 en 93 casos y T4 en 19. El promedio de ganglios positivos fue 3,4 (extremos 1-34). El índice ganglionar promedio fue 0,237 (DE: 0,197; extremos 0,031-0,882) y la mediana fue 0,1666. El 74% de los pacientes tenía 1 a 3 ganglios positivos (N1) y el 26% 4 o más ganglios positivos (N2). El seguimiento promedio fue de 67 meses (extremos 5-216), durante el cual fallecen 29 pacientes. El área bajo la curva ROC del número de LN afectados (0,703; IC 95%:0,58-082) fue levemente mayor que el área bajo la curva ROC del IG (0,690; IC 95%:0,57-0,81) (p = 0,63). Al compararlas en forma dicotómica, el IG (OR: 19,96; IC 95%:1,51-253,6) muestra una mayor capacidad de discriminación que el número de LN afectados (OR: 2,55; IC 95%: 0,86-7,55). Conclusión: El número de LN metastásicos y el IG son factores pronósticos relevantes en la planificación de la adyuvancia del cáncer de colon estadio III. Abstract in english Background: The lymph node ratio in malignant tumors corresponds to the ratio between the number of involved lymph nodes and the number of examined lymph nodes. This ratio may be a good prognostic index in stage III colon cancer. Aim: To compare the lymph node ratio with the absolute number of posit [...] ive lymph nodes as prognostic factors in stage III colon cancer. Material and Methods: Analysis of 115 patients aged 25 to 91 years (63% women) with a stage III colon cancer operated between 1991 and 2007. Survival according to the absolute number of positive lymph nodes and the lymph node index was calculated. The area under receiver operating characteristic (ROC) curves obtained after a COX regression analysis of survival, was used to analyze the prognostic value of each parameter. Results: Lymph node involvement was classified as T2 in three, T3 in 93 and T4 in 19 patients. The mean number of positive lymph nodes was 3.4 (range 1 to 34) and the mean lymph node index was 0.237 ± 0.197 (range 0.031-0.882). Seventy four percent of patients had one to three positive lymph nodes and 24% had more than three. During a mean follow up of 67 months (range 5-216), 29 patients died. In survival analysis, the area under the ROC curve for the number of involved lymph nodes (0.703, 95 confidence intervals (CI) 0.58-0.82) was slightly better than the area for lymph node index (0.69, 95% CI 0.57-0.81). Using a dichotomy analysis, a lymph node index over 0.31 had a higher discriminating value for survival (odds ratio (OR) 19.96 91% CI 1.51-253.6) than the presence of 12 or more involved lymph nodes (OR 2.55 95% CI 0.86-7.55). Conclusions: The lymph node index and the absolute number of involved lymph nodes are prognostic factors in stage III colon cancer.

GUILLERMO, BANNURA C; CLAUDIO, VARGAS S; ALEJANDRO, BARRERA E; CARLOS, MELO L; JAIME, CONTRERAS P.

2011-10-01

206

Guías Latinoamericanas de la RIICER para el diagnóstico de las rickettsiosis transmitidas por garrapatas / Latinamerican guidelines of RIICER for diagnosis of tick-borne rickettsioses  

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Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Las rickettsiosis transmitidas por garrapatas son afecciones de distribución mundial, que por diferentes motivos se pueden considerar emergentes y reemergentes. Hasta hace escasos años la única rickettsiosis transmitida por garrapatas en Latinoamérica era la infección por Rickettsia rickettsii, pero [...] en la actualidad y fundamentalmente, gracias a la incorporación de nuevas herramientas para el diagnóstico microbiológico como la reacción en cadena de la polimerasa y secuenciación o el cultivo celular rápido en tubo cerrado, se han descrito e involucrado otras especies de Rickettsia en la producción de patología humana. En estas guías se detallan y describen las diferentes técnicas utilizadas para el diagnóstico microbiológico de las rickettsiosis. Además, se incluye una sección en la que se detallan las especies más importantes de garrapatas duras relacionadas con las rickettsiosis en Latinoamérica, con claves para su clasificación taxonómica. Abstract in english Tick-borne rickettsioses are worldwide infectious diseases that are considered emerging and re-emerging. Until recently the only tick-borne rickettsiosis present in Latin America was Rickettsia rickettsii infection, but to date, with the incorporation of new tools as PCR and sequencing and the quick [...] cellular close tube cultures (Shell-vial), new species has been involved as human pathogens. In these guidelines, we offer an update of the microbiological assays for diagnosing rickettsioses. Besides we have included a section in which the most important hard ticks involved in human rickettsioses in Latinoamerica are detailed.

José A, Oteo; Santiago, Nava; Rita de, Sousa; Salim, Mattar; José M, Venzal; Katia, Abarca; Marcelo B, Labruna; Jorge, Zavala-Castro.

2014-02-01

207

Effects of Monocular Deprivation on the Dendritic Features of Retinal Ganglion Cells / Efectos de la Privación Monocular sobre las Características Dendríticas de las Células Ganglionares de la Retina  

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Full Text Available SciELO Chile | Language: English Abstract in spanish La privación monocular de la visión resulta en cambios anatómicos en la corteza visual en favor del ojo no privado. Aunque la retina forma parte de la vía visual, hay escasez de datos sobre el efecto de la privación monocular en su estructura. El objetivo de esta investigación fue describir los efec [...] tos de la privación monocular en las características de las dendritas de las células ganglionares de la retina. Se diseñó un estudio cuasi-experimental. Se examinaron 30 conejos (18 experimentales, 12 controles). La privación monocular se logró a través de la sutura unilateral del párpado en los animales de experimentación. Los conejos fueron observados durante tres semanas. Cada semana, 6 animales experimentales y 3 control fueron eutanasiados, donde se obtuvo la retina y fue procesada para realizar microscopía óptica. Las microfotografías de la retina fueron tomadas con una cámara digital y luego se utilizó el software FIJI para su análisis. El número de dendritas primarias, terminales y el área del campo de dendritas en los ojos no privados aumentó un 66,7% (p=0,385), 400% (p=0,002), y 88,4% (p=0,523), respectivamente. Los ojos no privados, tenían 114,3% más dendritas terminales (p=0,002) en comparación con los controles. Entre los ojos privados, todas las variables medidas tuvieron un aumento gradual en las dos primeras semanas, seguido de descenso con mayor privación. No se observaron diferencias estadísticamente significativas entre los ojos privados y el grupo control. En conclusion, la privación monocular produce un aumento de los contactos sinápticos en los ojos no privados, con cambios recíprocos que se manifiestan en los ojos privados de la visión. Abstract in english Monocular deprivation results in anatomical changes in the visual cortex in favor of the non-deprived eye. Although the retina forms part of the visual pathway, there is scarcity of data on the effect of monocular deprivation on its structure. The objective of this study was to describe the effects [...] of monocular deprivation on the retinal ganglion cell dendritic features. The study design was quasi-experimental. 30 rabbits (18 experimental, 12 controls) were examined. Monocular deprivation was achieved through unilateral lid suture in the experimental animals. The rabbits were observed for three weeks. Each week, 6 experimental and 3 control animals were euthanized, their retina harvested and processed for light microscopy. Photomicrographs of the retina were taken using a digital camera then entered into FIJI software for analysis. The number of primary branches, terminal branches and dendritic field area among the non-deprived eyes increased by 66.7%(p=0.385), 400%(p=0.002), and 88.4%(p=0.523) respectively. Non-deprived eyes had 114.3% more terminal dendrites (p=0.002) compared to controls. Among deprived eyes, all variables measured had a gradual rise in the first two weeks followed by decline with further deprivation. There were no statistically significant differences noted between the deprived and control eyes. Monocular deprivation results in increase in synaptic contacts in the non-deprived eye, with reciprocal changes occurring in the deprived eye.

Philip, Mwachaka; Hassan, Saidi; Paul, Odula; Pamela, Mandela.

1144-11-01

208

Distribution of Rickettsia rickettsii in ovary cells of Rhipicephalus sanguineus (Latreille1806 (Acari: Ixodidae  

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Full Text Available Abstract Background Considering the fact that the dog tick, Rhipicephalus sanguineus, has a great potential to become the vector of Brazilian Spotted Fever (BSF for humans, the present study aimed to describe the distribution of the bacterium Rickettsia rickettsii, the etiological agent of BSF, in different regions of the ovaries of R. sanguineus using histological techniques. The ovaries were obtained from positive females confirmed by the hemolymph test and fed in the nymph stage on guinea pigs inoculated with R. rickettsii. Results The results showed a general distribution of R. rickettsii in the ovary cells, being found in oocytes in all stages of development (I, II, III, IV and V most commonly in the periphery of the oocyte and also in the cytoplasm of pedicel cells. Conclusions The histological analysis of the ovaries of R. sanguineus infected females confirmed the presence of the bacterium, indicating that the infection can interfere negatively in the process of reproduction of the ticks, once alterations were detected both in the shape and cell structure of the oocytes which contained bacteria.

da Silva Costa Luís

2011-11-01

209

Molecular evidence of Ehrlichia canis and Rickettsia massiliae in ixodid ticks of carnivores from South Hungary.  

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To monitor the emergence of thermophilic, Mediterranean ixodid tick species and tick-borne pathogens in southern Hungary, 348 ticks were collected from shepherd dogs, red foxes and golden jackals during the summer of 2011. Golden jackals shared tick species with both the dog and the red fox in the region. Dermacentor nymphs were collected exclusively from dogs, and the sequence identification of these ticks indicated that dogs are preferred hosts of both D. reticulatus and D. marginatus nymphs, unlike previously reported. Subadults of three ixodid species were selected for reverse line blot hybridisation (RLB) analysis to screen their vector potential for 40 pathogens/groups. Results were negative for Anaplasma, Babesia and Theileria spp. Investigation of D. marginatus nymphs revealed the presence of Ehrlichia canis, Rickettsia massiliae and Borrelia afzelii for the first time in this tick species. These findings broaden the range of those tick-borne agents, which are typically transmitted by Rhipicephalus sanguineus, but may also have Dermacentor spp. as potential or alternative vectors. Ehrlichiacanis was also newly detected in Ixodes canisuga larvae from red foxes. In absence of transovarial transmission in ticks this implies that Eurasian red foxes may play a reservoir role in the epidemiology of canine ehrlichiosis. PMID:23439290

Hornok, Sándor; Fuente, José; Horváth, Gábor; Fernández de Mera, Isabel G; Wijnveld, Michiel; Tánczos, Balázs; Farkas, Róbert; Jongejan, Frans

2013-03-01

210

La detección de Bartonella spp. y Rickettsia spp. en pulgas, garrapatas y piojos recolectados en las zonas rurales de Perú / Detection of Bartonella spp. and Rickettsia spp. in fleas, ticks and lice collected in rural areas of Peru  

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Full Text Available SciELO Peru | Language: Spanish Abstract in spanish La Bartonellosis y la Rickettsiosis son enfermedades comúnmente reportadas en Perú. Con el propósito de detectar Bartonella sp. y Rickettsia sp. especímenes de pulgas, garrapatas y piojos de cinco localidades del Perú (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro) fueron colectadas y an [...] alizadas. Para la detección se usó PCR y una posterior confirmación con secuenciamiento de DNA. Los especímenes colectados fueron agrupados en 102 pools (76 Ctenocephalides felis, dos Ctenocephalides canis, 16Pulex irritans, cinco Pediculus humanus, dos Rhiphicephalus sanguineus, y un Boophilus spp.). Bartonella fue detectada en 17 pools (seis de C. felis, nueve de P. irritans, uno de C. canis, y uno de P. humanus). Rickettsia fue detectada en 76 pools (62 de C. felis, 10 de P. irritans, dos de P. humanus, y dos de C. canis). Bartonella clarridgeiae fue detectada en C. felis (5.3% especímenes), C. canis (50%) y P. irritans (12.5%). Bartonella rochalimae fue detectada en C. felis (1.3%) y P. irritans (12.5%). Además, se detectó B. henselae en C. felis (1.3%) y P. humanus (20%). Bartonella spp. también se encontró en P. irritans (31,3%). Además, se detectó R. felis en C. felis (76.3%), C. canis (100%) y P. irritans (37.5%), y Rickettsia spp. se detectó en C. felis (5,3%), P. irritans (25%) y P. humanus (40%). Estos resultados demuestran la circulación de estas bacterias en el Perú Abstract in english Bartonellosis and rickettsiosis are commonly reported in Peru. In order to detect Bartonella sp. and Rickettsia sp. in fleas, ticks and lice, specimens from five distinct locations in Peru (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro) were collected and screened for the presence of the [...] se bacteria using PCR and later confirmation by DNA sequencing. The specimens collected were distributed in 102 pools (76 Ctenocephalides felis, 2 Ctenocephalides canis, 16Pulex irritans, 5 Pediculus humanus, 2 Rhiphicephalus sanguineus, and 1 Boophilus spp.), whereBartonella was detected in 17 pools (6 of C. felis, 9 of P. irritans, 1 of C. canis, and 1 P. humanus). Also, Rickettsia was detected in 76 pools (62 C. felis, 10 P. irritans, 2 P. humanus, and 2 C. canis). Bartonella clarridgeiae was detected in C. felis, C. canis and P. irritans pools at 5.3%, 50% and 12.5%, respectively. Bartonella rochalimae was detected in one C. felis and two P. irritans pools at 1.3% and 12.5%, respectively. Furthermore, B. henselae was detected in one C. felis pool and one P. humanus pool corresponding to 1.3% and 20%, respectively; andBartonella spp. was also found in 5 pools of P. irritans at 31.3%. Additionally, R. felis was detected in C. felis, C. canis and P. irritans pools at 76.3%, 100% and 37.5%, respectively; and Rickettsia spp. was detected in C. felis, P. irritans and P. humanus pools at 5.3%, 25% and 40%, respectively. These results demonstrate the circulation of these bacteria in Peru

Abraham G, Cáceres; Carlos P, Padilla Rojas; Javier, Arias Stella; Gerardo, Huatuco Crisanto; Antero, Gonzales Pérez.

2013-08-01

211

Morphometric and quantitative characterization of atrial ganglion neurons from the intercaval region in dogs with dilated cardiomyopathy / Caracterização morfométrica e quantitativa dos neurônios ganglionares atriais da faixa intercaval de cães com cardiomiopatia dilatada  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese Avaliaram-se quantitativa, morfométrica e qualitativamente os neurônios atriais da faixa intercaval de cães com cardiomiopatia dilatada (CMD). Os neurônios dos gânglios nervosos de cães com CMD eram maiores que os dos cães controle. A histopatologia do miocárdio ventricular e dos neurônios gangliona [...] res confirmou a CMD e demonstrou evidente processo degenerativo neuronal ganglionar. Cães com CMD em fase crônica apresentavam cardioneuropatia secundária, provavelmente pela privação da inervação parassimpática cardíaca. Abstract in english The quantity, morphometry, and quality of atrial neurons from the intercaval region in dogs with dilated cardiomyopathy (DCM) were evaluated. Dogs with DCM had greater ganglion neurons than control dogs. The histologic evaluation of the ventricular myocardium and ganglion neurons confirmed DCM and s [...] howed the degeneration of ganglion neurons. Dogs with chronic DCM had a secondary cardioneuropathy owing to impaired parasympathetic neural control.

A.A., Camacho; R., Oliveira-Alves; R.P., Klein; M.G., Sousa.

1394-13-01

212

Short Report: Rickettsia felis Outer Membrane Protein A: A Potential Tool for Diagnosis of Patients with Flea-Borne Spotted Fever  

Science.gov (United States)

Rickettsia felis infection in humans has been detected worldwide, causing an illness that could be confused with other viral and bacterial infections such as dengue fever. Despite the high incidence of infection in humans, reservoirs, and vectors, the definitive diagnosis of R. felis infection currently requires polymerase chain reaction and sequencing because its antigens are closely related to other rickettsiae, making serologic diagnosis problematic. In this work, we report the immune reactivity of sera from four patients infected with R. felis with recombinant peptides representing regions of outer membrane protein A of R. felis. PMID:19052301

Zavala-Castro, Jorge E.; Dzul-Rosado, Karla R.; León, Juan José Arias; Walker, David H.; Zavala-Velázquez, Jorge E.

2014-01-01

213

A Molecular survey of Anaplasma spp., Rickettsia spp., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily.  

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Fleas (Insecta: Siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. Several species have been implicated in pathogen transmission. This study aimed to monitor red foxes and the fleas isolated from them in the Palermo and Ragusa provinces of Sicily, Italy, as these organisms are potential reservoirs and vectors of pathogens. Thirteen foxes (Vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (PCR) to detect DNA of the pathogens Ehrlichia canis, Babesia microti, Rickettsia spp., Anaplasma phagocytophilum, Anaplasma platys, Anaplasma marginale and Anaplasma ovis. In the foxes, A. ovis was detected in only one animal, whereas the prevalence of the E. canis pathogen was 31%. B. microti and Rickettsia spp. were not detected. Of all of the collected fleas, 75 belonged to the species Xenopsylla cheopis, 32 belonged to Ctenocephalides canis, two belonged to Ctenocephalides felis and one belonged to Cediopsylla inaequalis. In the fleas, the following pathogens were found: A. ovis (prevalence 25%), A. marginale (1%), A. phagocytophilum (1%), Rickettsia felis (2%) and E. canis (3%). X. cheopis was the flea species most frequently infected with Anaplasma, in particular A. ovis (33%), A. marginale (1%) and A. phagocytophilum (1%). Both C. felis exemplars were positive for R. felis. E. canis was found in the lone C. inaequalis and also in 3% of the X. cheopis specimens. No fleas were positive for B. microti or A. platys. As foxes often live in proximity to domestic areas, they may constitute potential reservoirs for human and animal parasites. Further studies should be performed on fleas to determine their vectorial capacity. PMID:24589112

Torina, A; Blanda, V; Antoci, F; Scimeca, S; D'Agostino, R; Scariano, E; Piazza, A; Galluzzo, P; Giudice, E; Caracappa, S

2013-11-01

214

Evaluation of Rickettsia japonica pathogenesis and reservoir potential in dogs by experimental inoculation and epidemiologic survey.  

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Rickettsia japonica pathogenesis and reservoir potential in dogs were evaluated by both experimental inoculation and epidemiologic survey. In the experimental inoculation study, dogs 1 and 2 were pretreated with an immunosuppressive dose of cyclosporine 14 days before inoculation and became ill after exposure to R. japonica. Dogs exhibited clinical signs, including fever, anorexia, depression, and decreased water consumption, between 36 and 96 h after inoculation, but these signs disappeared spontaneously by 5 days after inoculation. Dogs 3 and 4 were not pretreated with cyclosporine, and no clinical signs were detected in them throughout the 14-day observation period. The control dog was clinically normal and had a normal rectal temperature throughout the study period. We attempted to detect rickettsial DNA from peripheral blood and aspiration samples from kidney and spleen by nested PCR, but all samples examined were negative. The control dog lacked detectable titers to R. japonica antigen on day 14, while positive antibodies to R. japonica were detected in all four experimentally infected dogs, with titers of 1:160 to 1:80. In the epidemiologic survey, 24 (1.8%) of the 1,363 dogs examined throughout Japan had antibodies against R. japonica, with titers of 1:40 or more. However, we observed neither clinical signs at the time of sample collection nor nested PCR results indicative of rickettsial infection in these dogs. In conclusion, dogs in Japan can be exposed to R. japonica, and infected dogs with immunosuppressive conditions can temporarily develop clinical symptoms, including fever, anorexia, depression, and decreased water consumption. PMID:20980481

Inokuma, Hisashi; Matsuda, Hironori; Sakamoto, Leo; Tagawa, Michihito; Matsumoto, Kotaro

2011-01-01

215

A Molecular Survey of Rickettsia felis in Fleas from Cats and Dogs in Sicily (Southern Italy)  

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Rickettsia felis, the agent of flea-borne spotted fever, has a cosmopolitan distribution. Its pathogenic role in humans has been demonstrated through molecular and serologic tests in several cases. The cat flea (Ctenocephalides felis) is considered the main reservoir and the biological vector. The aim of this study was to assess the presence and occurrence of R. felis in fleas collected from dogs and cats in various sites of Palermo (Sicily). Between August and October 2012, 134 fleas were collected from 42 animals: 37 fleas from 13 dogs and 97 fleas from 29 cats. Two species of fleas were identified: 132 Ctenocephalides felis (98.51%) collected on all animals and only two C. canis (1.49%) on one dog. Out of 132 C. felis, 34 (25.76%), 12 from dogs (32.43%) and 22 (22.68%) from cats, were positive for R. felis DNA by a polymerase chain reaction (PCR), confirmed by sequencing. The only two C. canis fleas were negative. About half of examined animals (47.62%, 20/42) were infested with at least one infected flea; in particular 46.15% of dogs (6/13) and 48.28% of cats (14/29). It seems that in the Palermo district there is a peri-domestic cycle, with a relatively high prevalence of R. felis infection in the cat flea, an insect widely diffused in home environments and which can frequently bite humans. The results also suggest that R. felis should be considered in the human differential diagnosis of any spotted-like fever or febrile illness without a clear source of infection in Sicily, especially if the patient is known to have been exposed to flea bites. PMID:25203839

Giudice, Elisabetta; Di Pietro, Simona; Alaimo, Antonio; Blanda, Valeria; Lelli, Rossella; Francaviglia, Francesco; Caracappa, Santo; Torina, Alessandra

2014-01-01

216

Associations between coinfection prevalence of Borrelia lusitaniae, Anaplasma sp., and Rickettsia sp. in hard ticks feeding on reptile hosts.  

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An increasing number of studies reveal that ticks and their hosts are infected with multiple pathogens, suggesting that coinfection might be frequent for both vectors and wild reservoir hosts. Whereas the examination of associations between coinfecting pathogen agents in natural host-vector-pathogen systems is a prerequisite for a better understanding of disease maintenance and transmission, the associations between pathogens within vectors or hosts are seldom explicitly examined. We examined the prevalence of pathogen agents and the patterns of associations between them under natural conditions, using a previously unexamined host-vector-pathogen system--green lizards Lacerta viridis, hard ticks Ixodes ricinus, and Borrelia, Anaplasma, and Rickettsia pathogens. We found that immature ticks infesting a temperate lizard species in Central Europe were infected with multiple pathogens. Considering I. ricinus nymphs and larvae, the prevalence of Anaplasma, Borrelia, and Rickettsia was 13.1% and 8.7%, 12.8% and 1.3%, and 4.5% and 2.7%, respectively. The patterns of pathogen prevalence and observed coinfection rates suggest that the risk of tick infection with one pathogen is not independent of other pathogens. Our results indicate that Anaplasma can play a role in suppressing the transmission of Borrelia to tick vectors. Overall, however, positive effects of Borrelia on Anaplasma seem to prevail as judged by higher-than-expected Borrelia-Anaplasma coinfection rates. PMID:20711724

Václav, Radovan; Ficová, Martina; Prokop, Pavol; Betáková, Tatiana

2011-02-01

217

Prevalence of antibodies to spotted fever group Rickettsia spp. and Ehrlichia spp. in coyotes (Canis latrans) in Oklahoma and Texas, USA.  

Science.gov (United States)

Coyotes (Canis latrans) are commonly infested with ticks, including Amblyomma americanum, the predominant vector of Ehrlichia chaffeensis and Ehrlichia ewingii; Dermacentor variabilis, an important vector of Rickettsia rickettsii; and Amblyomma maculatum, a major vector of Rickettsia parkeri, a spotted fever group (SFG) Rickettsia. To determine the degree to which coyotes are infected with or exposed to tick-borne bacterial disease agents, serum samples collected from coyotes in Oklahoma and Texas were tested for antibodies reactive to R. rickettsii, Ehrlichia canis, E. chaffeensis, E. ewingii, Borrelia burgdorferi, and Anaplasma phagocytophilum by indirect fluorescent antibody (IFA) testing or enzyme-linked immunosorbent assay (ELISA). Of the coyotes tested, 60% (46/77) and 64% (47/74) had antibodies reactive to R. rickettsii and E. chaffeensis, respectively, on IFA. Additionally, 5% (4/77) had antibodies reactive to E. canis, but not B. burgdorferi or A. phagocytophilum, on SNAP(®) 4Dx(®) ELISA; subsequent serologic analysis by plate ELISA using species-specific peptides revealed antibodies to E. ewingii, E. canis, and E. chaffeensis in 46% (23/50), 18% (9/50), and 4% (2/50) of serum samples, respectively. Taken together, these data indicate that coyotes in this region are commonly exposed to SFG Rickettsia and E. ewingii and that further consideration of coyotes as a component of the maintenance cycle for these pathogens may be warranted. PMID:23778619

Starkey, Lindsay A; West, Misti D; Barrett, Anne W; Saucier, Jill M; O'Connor, Tom P; Paras, Kelsey L; Reiskind, Michael H; Reichard, Mason V; Little, Susan E

2013-07-01

218

Pathogens and symbionts in ticks: prevalence of Anaplasma phagocytophilum (Ehrlichia sp.), Wolbachia sp., Rickettsia sp., and Babesia sp. in Southern Germany.  

Science.gov (United States)

Tick-transmitted diseases like tick-borne encephalitis and Lyme borreliosis have been well known in Germany for decades. Ongoing research now gives an additional focus to a broad range of other bacteria and parasites in ticks like Anaplasma phagocytophilum, former Ehrlichia sp., Rickettsia sp. and Babesia sp. Knowledge about the prevalence of these infectious agents in ticks is an important prerequisite for risk assessment of human diseases. Therefore nymphs and adult Ixodes ricinus ticks were collected and examined for Anaplasma phagocytophilum (n = 5424 ticks), Rickettsia sp. (n = 1187), and Babesia sp. (n = 3113). For the detection of Anaplasma phagocytophilum, DNA from the 16S rDNA gene was amplified by nested PCR and hybridized with a DIG-labeled oligonucleotide probe. The examination of Rickettsia sp. was performed by single PCR. A partial sequence of the citrate synthase gene was amplified. As a target for the detection of Babesia sp., DNA from the 18S rDNA gene was amplified, also by single PCR. All positive PCR products were sequenced to control specificity. Anaplasma phagocytophilum was detected by PCR in n = 103 (1.9%) out of 5,424 examined ticks from 11 investigation areas. However, not all positive PCR products hybridized using DIG-labeled oligonucleotide probe. Thus, the result of sequencing indicated that only 1.0% (n = 54) belonged to Anaplasma phagocytophilum and nearly half of these PCR products (0.9%) were identified as Wolbachia sp. Rickettsia sp. in Ixodes ricinus ticks from 3 areas were found in n = 105 (8.9%) out of 1,187 ticks examined (range from 13.3% to 5.6%). Sequencing showed Rickettsia helvetica exclusively. In about 2.6% of Rickettsia-positive ticks, double infection with Anaplasma phagocytophilum was found. Babesia sp. was detected in n= 31 (1.0%) out of 3,113 ticks examined, which originated from 4 different areas. By sequencing, n = 28 (90.0%) were identified as Babesia divergens. Three of all Babesia-positive ticks were identified as harboring Babesia microti. The detection of Anaplasma phagocytophilum, Rickettsia sp. and Babesia sp. demonstrates their possible role as a source of human infection in Germany. PMID:15146989

Hartelt, Kathrin; Oehme, Rainer; Frank, Henning; Brockmann, Stefan O; Hassler, Dieter; Kimmig, Peter

2004-04-01

219

Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted fever in the city of Rio de Janeiro, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english A lethal case of Brazilian spotted fever (BSF) is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA) for spotted fever group rickett [...] sia (SFGR) was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR) and the nucleotide sequencing of a fragment of the ompA gene showed 100% homology to Rickettsia rickettsii. BSF has not been reported in the city of Rio de Janeiro in the last three decades, and the present description should alert the clinicians to its presence in urban Rio de Janeiro, and to the differential diagnosis with dengue fever, gastroenteritis, leptospirosis and bacterial septic shock, among others.

Cristiane, Lamas; Alexsandra, Favacho; Tatiana, Rozental; Márcio N., Bóia; Andrei H., Kirsten; Alexandro, Guterres; Jairo, Barreira; Elba Regina S. de, Lemos.

2008-04-01

220

Rickettsia parkeri rickettsiosis in different ecological regions of Argentina and its association with Amblyomma tigrinum as a potential vector.  

Science.gov (United States)

Rickettsia parkeri, a newly recognized tick-borne pathogen of humans in the Americas, is a confirmed cause of spotted fever group rickettsiosis in Argentina. Until recently, almost all cases of R. parkeri rickettsiosis in Argentina have originated from the Paraná River Delta, where entomological surveys have identified populations of R. parkeri-infected Amblyomma triste ticks. In this report, we describe confirmed cases of R. parkeri rickettsiosis from Córdoba and La Rioja provinces, which are located several hundred kilometers inland, and in a more arid ecological region, where A. triste ticks do not occur. Additionally, we identified questing A. tigrinum ticks naturally infected with R. parkeri in Córdoba province. These data provide evidence that another human-biting tick species serves as a potential vector of R. parkeri in Argentina and possibly, other countries of South America. PMID:25349376

Romer, Yamila; Nava, Santiago; Govedic, Francisco; Cicuttin, Gabriel; Denison, Amy M; Singleton, Joseph; Kelly, Aubree J; Kato, Cecilia Y; Paddock, Christopher D

2014-12-01

221

Análisis de las recidivas locorregionales por cáncer de mama / Analysis of locoregional relapses from breast cancer  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Introducción: la recidiva locorregional, luego de una intervención quirúrgica por cáncer de mama, es una gran preocupación para el paciente y una frustración para el cirujano actuante. Objetivos: realizar un estudio descriptivo de las variables asociadas a la recurrencia tumoral, en 949 pacientes op [...] eradas. Métodos: se confeccionó una base de datos con las pacientes operadas desde 2005 hasta 2010, se identificaron las variables y análisis univariables y multivariables para determinar si existió asociación estadística. Resultados: se apreció que la incidencia fue de 4,4 %. La intervención quirúrgica previa más frecuente fue la mastectomía radical modificada en 52,4 %, y 26,2 % de los casos que tenían metástasis ganglionares axilares en ese momento. El tipo histológico más frecuente fue el carcinoma ductal infiltrante, de alto grado de malignidad. La infiltración vascular y linfática estuvo presente en 42,8 %. El tratamiento definitivo más empleado fue la exéresis de la recidiva más radioterapia. El análisis multivariables solo asoció la metástasis ganglionar con la reaparición de la enfermedad. Conclusiones: la metástasis ganglionar axilar sigue siendo un factor de mal pronóstico en el seguimiento extenso de la paciente. Abstract in english Introduction: locoregional relapses after a breast cancer surgery is a big concern for the patient and a frustration to the performing surgeon. Objectives: to perform a descriptive study of the tumor recurrence-associated variables in 949 surgical patients. Methods: a database containing the data fr [...] om operated patients in the period of 2005 through 2010 was made; the variables were identified and the univariate and multivariate analyses allowed determining the probable statistical association. Results: at was observed that the incidence of locoregional recurrence was 4.4 %. The most common previous surgery was modified radical mastectomy in 52.4 % and 26.2 % of cases with axillary ganglionic metastasis at that time. The most frequent histological type was highly malignant infiltrating bile duct carcinoma. The vascular and lymphatic infiltration occurred in 42.8 % of patients. The most used treatment was removal of recurrence plus radiotherapy. The multivariate analysis was associated only to ganglionic metastasis with the reappearance of disease. Conclusions: axillary ganglionic metastasis is still a factor indicating bad prognosis in the long follow-up of a patient.

Oscar Alberto, Pérez Gutiérrez; Narciso, Montejo Viamontes; Loys, Jorge Lázaro; Rafael, Castro Cruz; Amauris, Estrada González.

2012-12-01

222

Análisis de las recidivas locorregionales por cáncer de mama Analysis of locoregional relapses from breast cancer  

Directory of Open Access Journals (Sweden)

Full Text Available Introducción: la recidiva locorregional, luego de una intervención quirúrgica por cáncer de mama, es una gran preocupación para el paciente y una frustración para el cirujano actuante. Objetivos: realizar un estudio descriptivo de las variables asociadas a la recurrencia tumoral, en 949 pacientes operadas. Métodos: se confeccionó una base de datos con las pacientes operadas desde 2005 hasta 2010, se identificaron las variables y análisis univariables y multivariables para determinar si existió asociación estadística. Resultados: se apreció que la incidencia fue de 4,4 %. La intervención quirúrgica previa más frecuente fue la mastectomía radical modificada en 52,4 %, y 26,2 % de los casos que tenían metástasis ganglionares axilares en ese momento. El tipo histológico más frecuente fue el carcinoma ductal infiltrante, de alto grado de malignidad. La infiltración vascular y linfática estuvo presente en 42,8 %. El tratamiento definitivo más empleado fue la exéresis de la recidiva más radioterapia. El análisis multivariables solo asoció la metástasis ganglionar con la reaparición de la enfermedad. Conclusiones: la metástasis ganglionar axilar sigue siendo un factor de mal pronóstico en el seguimiento extenso de la paciente.Introduction: locoregional relapses after a breast cancer surgery is a big concern for the patient and a frustration to the performing surgeon. Objectives: to perform a descriptive study of the tumor recurrence-associated variables in 949 surgical patients. Methods: a database containing the data from operated patients in the period of 2005 through 2010 was made; the variables were identified and the univariate and multivariate analyses allowed determining the probable statistical association. Results: at was observed that the incidence of locoregional recurrence was 4.4 %. The most common previous surgery was modified radical mastectomy in 52.4 % and 26.2 % of cases with axillary ganglionic metastasis at that time. The most frequent histological type was highly malignant infiltrating bile duct carcinoma. The vascular and lymphatic infiltration occurred in 42.8 % of patients. The most used treatment was removal of recurrence plus radiotherapy. The multivariate analysis was associated only to ganglionic metastasis with the reappearance of disease. Conclusions: axillary ganglionic metastasis is still a factor indicating bad prognosis in the long follow-up of a patient.

Oscar Alberto Pérez Gutiérrez

2012-12-01

223

Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands  

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Full Text Available Abstract Background Awareness for flea- and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The role of Ixodes ricinus ticks in the natural cycle of Bartonella spp. and the transmission of these bacteria to humans is unclear. Rickettsia spp. have also been reported from as well ticks as also from fleas. However, to date no flea-borne Rickettsia spp. were reported from the Netherlands. Here, the presence of Bartonellaceae and Rickettsiae in ectoparasites was investigated using molecular detection and identification on part of the gltA- and 16S rRNA-genes. Results The zoonotic Bartonella clarridgeiae and Rickettsia felis were detected for the first time in Dutch cat fleas. B. henselae was found in cat fleas and B. schoenbuchensis in ticks and keds feeding on deer. Two Bartonella species, previously identified in rodents, were found in wild mice and their fleas. However, none of these microorganisms were found in 1719 questing Ixodes ricinus ticks. Notably, the gltA gene amplified from DNA lysates of approximately 10% of the questing nymph and adult ticks was similar to that of an uncultured Bartonella-related species found in other hard tick species. The gltA gene of this Bartonella-related species was also detected in questing larvae for which a 16S rRNA gene PCR also tested positive for "Candidatus Midichloria mitochondrii". The gltA-gene of the Bartonella-related species found in I. ricinus may therefore be from this endosymbiont. Conclusions We conclude that the risk of acquiring Cat Scratch Disease or a related bartonellosis from questing ticks in the Netherlands is negligible. On the other hand fleas and deer keds are probable vectors for associated Bartonella species between animals and might also transmit Bartonella spp. to humans.

Reimerink Johan R

2011-04-01

224

Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands  

OpenAIRE

BACKGROUND: Awareness for flea- and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The role of Ixodes ricinus ticks in the natural cycle of Bartonella spp. and the transmission of these bacteria to humans is unclear. Rickettsia spp. have also been reported from as well ticks as also ...

Tijsse-klasen, E.; Fonville, M.; Gassner, F.; Nijhof, A. M.; Hovius, E. K.; Jongejan, F.; Takken, W.; Reimerink, J. R.; Overgaauw, P. A. M.; Sprong, H.

2011-01-01

225

Rickettsia peacockii, an endosymbiont of Dermacentor andersoni, does not elicit or inhibit humoral immune responses from immunocompetent D. andersoni or Ixodes scapularis cell lines  

OpenAIRE

Ixodes scapularis and Dermacentor andersoni cell lines were stimulated with heat killed Escherichia coli and Micrococcus luteus to investigate whether infection by Rickettsia peacockii, an endosymbiont of D. andersoni, modifies humoral immune responses. Radial diffusion assays, western blotting, flow cytometry, and quantitative reverse-transcription PCR were used to determine if expression of bacteriolytic peptides, including lysozyme and defensin, was upregulated by bacterial stimulation or ...

Mattila, Joshua T.; Munderloh, Ulrike G.; Kurtti, Timothy J.

2007-01-01

226

Dose-response model of murine typhus (Rickettsia typhi): time post inoculation and host age dependency analysis  

OpenAIRE

Abstract Background Rickettsia typhi (R. mooseri) is the causative agent of murine typhus. It is one of the most widely distributed flea-borne diseases with a relatively mild febrile initial illness with six to 14 days of incubation period. The bacterium is gram negative and an obligate intracellular pathogen. The disease is transmitted to humans and vertebrate host through fleabites or via contact with infected feces. This paper develops dose-response models of diff...

Tamrakar Sushil B; Huang Yin; Teske Sondra S; Haas Charles N

2012-01-01

227

Loop-mediated isothermal amplification for Rickettsia typhi (the causal agent of murine typhus): Problems with diagnosis at the limit of detection  

OpenAIRE

Murine typhus is a flea-borne disease of worldwide distribution caused by Rickettsia typhi. Although treatment with tetracycline antibiotics is effective, treatment is often misguided or delayed due to diagnostic difficulties. As the gold standard immunofluorescence assay is imperfect, we aimed to develop and evaluate a loop-mediated isothermal amplification (LAMP) assay. LAMP assays have the potential to fulfill the WHO ASSURED criteria (affordable, sensitive, specific, user friendly, robust...

Dittrich, S.; Castonguay-vanier, J.; Moore, Ce; Thongyoo, N.; Newton, Pn; Paris, DH

2014-01-01

228

Serological Reactivity and Biochemical Characterization of Methylated and Unmethylated Forms of a Recombinant Protein Fragment Derived from Outer Membrane Protein B of Rickettsia typhi?  

OpenAIRE

Rickettsia typhi, an obligate intracellular bacterium that causes murine typhus, possesses a heavily methylated outer membrane protein B (OmpB) antigen. This immunodominant antigen is responsible for serological reactions and is capable of eliciting protective immune responses with a guinea pig model. Western blot analysis of partially digested OmpB with patient sera revealed that most of the reactive fragments are larger than 20 kDa. One of these fragments, which is located at the N terminus...

Chao, Chien-chung; Zhang, Zhiwen; Wang, Hui; Alkhalil, Abdulnaser; Ching, Wei-mei

2008-01-01

229

Distribution of Rickettsia rickettsii in ovary cells of Rhipicephalus sanguineus (Latreille1806) (Acari: Ixodidae)  

OpenAIRE

Abstract Background Considering the fact that the dog tick, Rhipicephalus sanguineus, has a great potential to become the vector of Brazilian Spotted Fever (BSF) for humans, the present study aimed to describe the distribution of the bacterium Rickettsia rickettsii, the etiological agent of BSF, in different regions of the ovaries of R. sanguineus using histological techniques. The ovaries were obtained from positive females confirmed by the hemolym...

da Silva Costa Luís; Nunes Pablo; Soares João; Labruna Marcelo; Camargo-Mathias Maria

2011-01-01

230

Occurrence of Babesia spp., Rickettsia spp. and Bartonella spp. in Ixodes ricinus in Bavarian public parks, Germany  

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Full Text Available Abstract Background Only limited information is available about the occurrence of ticks and tick-borne pathogens in public parks, which are areas strongly influenced by human beings. For this reason, Ixodes ricinus were collected in public parks of different Bavarian cities in a 2-year survey (2009 and 2010 and screened for DNA of Babesia spp., Rickettsia spp. and Bartonella spp. by PCR. Species identification was performed by sequence analysis and alignment with existing sequences in GenBank. Additionally, coinfections with Anaplasma phagocytophilum were investigated. Results The following prevalences were detected: Babesia spp.: 0.4% (n = 17, including one pool of two larvae in 2009 and 0.5 to 0.7% (n = 11, including one pool of five larvae in 2010; Rickettsia spp.: 6.4 to 7.7% (n = 285, including 16 pools of 76 larvae in 2009. DNA of Bartonella spp. in I. ricinus in Bavarian public parks could not be identified. Sequence analysis revealed the following species: Babesia sp. EU1 (n = 25, B. divergens (n = 1, B. divergens/capreoli (n = 1, B. gibsoni-like (n = 1, R. helvetica (n = 272, R. monacensis IrR/Munich (n = 12 and unspecified R. monacensis (n = 1. The majority of coinfections were R. helvetica with A. phagocytophilum (n = 27, but coinfections between Babesia spp. and A. phagocytophilum, or Babesia spp. and R. helvetica were also detected. Conclusions I. ricinus ticks in urban areas of Germany harbor several tick-borne pathogens and coinfections were also observed. Public parks are of particularly great interest regarding the epidemiology of tick-borne pathogens, because of differences in both the prevalence of pathogens in ticks as well as a varying species arrangement when compared to woodland areas. The record of DNA of a Babesia gibsoni-like pathogen detected in I. ricinus suggests that I. ricinus may harbor and transmit more Babesia spp. than previously known. Because of their high recreational value for human beings, urban green areas are likely to remain in the research focus on public health issues.

Mahling Monia

2011-07-01

231

Molecular identification of Rickettsia parkeri infecting Amblyomma triste ticks in an area of Argentina where cases of rickettsiosis were diagnosed  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Specimens of the hard tick Amblyomma triste were found infected with Rickettsia parkeri in an area of Argentina (General Lavalle, Buenos Aires Province) where cases of human illness attributed to this microorganism have been reported. Molecular detection of R. parkeri was based on polymerase chain r [...] eactions that amplify a ca. 400-bp fragment of the 23S-5S intergenic spacer and a ca. 500-bp fragment of the gene encoding a 190-kDa outer membrane protein. Three (6.97%) of 43 A. triste ticks were determined to be positive for R. parkeri. These results provide strong evidence that A. triste is the vector of R. parkeri in the study area. The findings of this work have epidemiological relevance because human parasitism by A. triste ticks has been frequently recorded in some riparian areas of Argentina and Uruguay and new cases of R. parkeri rickettsiosis might arise in the South American localities where humans are exposed to the bites of this tick species.

Gabriel, Cicuttin; Santiago, Nava.

2013-02-01

232

Molecular identification of Rickettsia parkeri infecting Amblyomma triste ticks in an area of Argentina where cases of rickettsiosis were diagnosed  

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Full Text Available Specimens of the hard tick Amblyomma triste were found infected with Rickettsia parkeri in an area of Argentina (General Lavalle, Buenos Aires Province where cases of human illness attributed to this microorganism have been reported. Molecular detection of R. parkeri was based on polymerase chain reactions that amplify a ca. 400-bp fragment of the 23S-5S intergenic spacer and a ca. 500-bp fragment of the gene encoding a 190-kDa outer membrane protein. Three (6.97% of 43 A. triste ticks were determined to be positive for R. parkeri. These results provide strong evidence that A. triste is the vector of R. parkeri in the study area. The findings of this work have epidemiological relevance because human parasitism by A. triste ticks has been frequently recorded in some riparian areas of Argentina and Uruguay and new cases of R. parkeri rickettsiosis might arise in the South American localities where humans are exposed to the bites of this tick species.

Gabriel Cicuttin

2013-02-01

233

Molecular identification of Rickettsia parkeri infecting Amblyomma triste ticks in an area of Argentina where cases of rickettsiosis were diagnosed.  

Science.gov (United States)

Specimens of the hard tick Amblyomma triste were found infected with Rickettsia parkeri in an area of Argentina (General Lavalle, Buenos Aires Province) where cases of human illness attributed to this microorganism have been reported. Molecular detection of R. parkeri was based on polymerase chain reactions that amplify a ca. 400-bp fragment of the 23S-5S intergenic spacer and a ca. 500-bp fragment of the gene encoding a 190-kDa outer membrane protein. Three (6.97%) of 43 A. triste ticks were determined to be positive for R. parkeri. These results provide strong evidence that A. triste is the vector of R. parkeri in the study area. The findings of this work have epidemiological relevance because human parasitism by A. triste ticks has been frequently recorded in some riparian areas of Argentina and Uruguay and new cases of R. parkeri rickettsiosis might arise in the South American localities where humans are exposed to the bites of this tick species. PMID:23440128

Cicuttin, Gabriel; Nava, Santiago

2013-02-01

234

Dual exposure of Rickettsia typhi and Orientia tsutsugamushi in the field-collected Rattus rodents from Thailand.  

Science.gov (United States)

Field-collected rodents and fleas from ten provinces covering four regions of Thailand were investigated for possible rickettsial pathogen infections. The 257 trapped-rodents belonged to 12 species. Five species of Genus Rattus accounted for 93% of the total capture, of which Rattus exulans and Rattus norvegicus were the two major species caught. All flea specimens, removed from trapped rodents, were identified as Xenopsylla cheopis. The PCR technique was performed on ectoparasite specimens to detect the presence of murine typhus pathogen (Rickettsia typhi) and scrub typhus pathogen (Orientia tsutsugamushi). Thirteen flea specimens (2.6 %) were found to be positive for R. typhi but none for O. tsutsugamushi. An ELISA technique was used to detect the rodent's antibodies against R. typhi and O. tsutsugamushi. Sixty-one rodent serum samples (23.7%) were positive for R. typhi specific IgM, IgG, or both, while 47 of the samples (18.3%) were positive for O. tsutsugamushi. Twenty serum samples from R. norvegicus (7.8%) had detectable antibodies against both R. typhi and O. tsutsugamushi. Our findings revealed the existence of the dual infection of rickettsial pathogens in the same natural hosts. PMID:24820571

Chareonviriyaphap, Theeraphap; Leepitakrat, Warinpassorn; Lerdthusnee, Kriangkrai; Chao, Chien Chung; Ching, Wei Mei

2014-06-01

235

In vitro isolation and infection intensity of Rickettsia parkeri in Amblyomma triste ticks from the Paraná River Delta region, Argentina.  

Science.gov (United States)

In the present study, we report the first in vitro isolation and infection intensity of Rickettsia parkeri in Amblyomma triste ticks from Argentina. No genetic differences in the molecular targets evaluated were found between R. parkeri isolates from Argentina and those R. parkeri isolates reported in Uruguay and Brazil, both obtained from A. triste. Only a minor difference was observed when compared to R. parkeri isolated from Amblyomma maculatum from United States. Moreover, the prevalence of infection by R. parkeri in ticks collected from the vegetation in the Paraná Delta was high (20.4%). Interestingly, the distribution of R. parkeri infection intensity observed in A. triste ticks was distinctly bimodal, with approximately 60% of the infected ticks presenting high rickettsial loads (3.8×10(5)-4.5×10(7) ompA copies/tick) and the remainder with low rickettsial levels (5.6×10(1)-6.5×10(3) ompA copies/tick). This bimodality in R. parkeri infection intensity in ticks could determine differences in the severity of the disease, but also be important for the infection dynamics of this pathogen. Further research exploring the distribution of rickettsial infection levels in ticks, as well as its determinants and implications, is warranted. PMID:25113981

Monje, Lucas D; Nava, Santiago; Antoniazzi, Leandro R; Colombo, Valeria C; Beldomenico, Pablo M

2014-10-01

236

'Candidatus Megaira polyxenophila' gen. nov., sp. nov.: considerations on evolutionary history, host range and shift of early divergent rickettsiae.  

Science.gov (United States)

"Neglected Rickettsiaceae" (i.e. those harboured by non-hematophagous eukaryotic hosts) display greater phylogenetic variability and more widespread dispersal than pathogenic ones; yet, the knowledge about their actual host range and host shift mechanism is scarce. The present work reports the characterization following the full-cycle rRNA approach (SSU rRNA sequence, specific in situ hybridization, and ultrastructure) of a novel rickettsial bacterium, herewith proposed as 'Candidatus Megaira polyxenophila' gen. nov., sp. nov. We found it in association with four different free-living ciliates (Diophrys oligothrix, Euplotes octocarinatus, Paramecium caudatum, and Spirostomum sp., all belonging to Alveolata, Ciliophora); furthermore it was recently observed as intracellular occurring in Carteria cerasiformis and Pleodorina japonica (Chlorophyceae, Chlorophyta). Phylogenetic analyses demonstrated the belonging of the candidate new genus to the family Rickettsiaceae (Alphaproteobacteria, Rickettsiales) as a sister group of the genus Rickettsia. In situ observations revealed the ability of the candidate new species to colonize either nuclear or cytoplasmic compartments, depending on the host organism. The presence of the same bacterial species within different, evolutionary distant, hosts indicates that 'Candidatus Megaira polyxenophila' recently underwent several distinct host shifts, thus suggesting the existence of horizontal transmission pathways. We consider these findings as indicative of an unexpected spread of rickettsial infections in aquatic communities, possibly by means of trophic interactions, and hence propose a new interpretation of the origin and phylogenetic diversification of rickettsial bacteria. PMID:23977321

Schrallhammer, Martina; Ferrantini, Filippo; Vannini, Claudia; Galati, Stefano; Schweikert, Michael; Görtz, Hans-Dieter; Verni, Franco; Petroni, Giulio

2013-01-01

237

‘Candidatus Megaira polyxenophila’ gen. nov., sp. nov.: Considerations on Evolutionary History, Host Range and Shift of Early Divergent Rickettsiae  

Science.gov (United States)

“Neglected Rickettsiaceae” (i.e. those harboured by non-hematophagous eukaryotic hosts) display greater phylogenetic variability and more widespread dispersal than pathogenic ones; yet, the knowledge about their actual host range and host shift mechanism is scarce. The present work reports the characterization following the full-cycle rRNA approach (SSU rRNA sequence, specific in situ hybridization, and ultrastructure) of a novel rickettsial bacterium, herewith proposed as 'Candidatus Megaira polyxenophila' gen. nov., sp. nov. We found it in association with four different free-living ciliates (Diophrys oligothrix, Euplotes octocarinatus, Paramecium caudatum, and Spirostomum sp., all belonging to Alveolata, Ciliophora); furthermore it was recently observed as intracellular occurring in Carteria cerasiformis and Pleodorina japonica (Chlorophyceae, Chlorophyta). Phylogenetic analyses demonstrated the belonging of the candidate new genus to the family Rickettsiaceae (Alphaproteobacteria, Rickettsiales) as a sister group of the genus Rickettsia. In situ observations revealed the ability of the candidate new species to colonize either nuclear or cytoplasmic compartments, depending on the host organism. The presence of the same bacterial species within different, evolutionary distant, hosts indicates that 'Candidatus Megaira polyxenophila' recently underwent several distinct host shifts, thus suggesting the existence of horizontal transmission pathways. We consider these findings as indicative of an unexpected spread of rickettsial infections in aquatic communities, possibly by means of trophic interactions, and hence propose a new interpretation of the origin and phylogenetic diversification of rickettsial bacteria. PMID:23977321

Vannini, Claudia; Galati, Stefano; Schweikert, Michael; Görtz, Hans-Dieter; Verni, Franco; Petroni, Giulio

2013-01-01

238

La radioterapia en el cáncer de próstata localmente avanzado tras estadiaje ganglionar con linfadenectomía / External beam radiotherapy on locally advanced prostate carcinoma following iliac staging lymphadenectomy  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Introducción y objetivos: El cáncer de próstata localmente avanzado supone una condición de alto riesgo de progresión bioquímica post-tratamiento debido a la situación límite que representa. Nos planteamos analizar los factores pronósticos clínicos en función de la probabilidad de progresión tras em [...] plear un tratamiento con fuente radioterapeútica externa en pacientes con este tipo de tumores. Material y métodos: Revisamos retrospectivamente una serie de 128 pacientes sometidos a linfadenectomía pélvica de estadiaje como método de estadiaje previo a la realización de el tratamiento radioterápico. Empleamos las curvas de Kaplan-Meier para estudiar la probabilidad de progresión, el test de rangos logarítmicos para la demostración de posibles diferencias estadísticamente significativas y el modelo de riesgos proporcionales de Cox para estudiar los posibles factores pronósticos implicados. Resultados: La probabilidad de no progresión a 5 años ha sido del 49,93%; a pesar de apreciar diferencias importantes en los grupos estratificados por las variables predictivas empleadas (PSA total, gleason de biopsia, estadio patológico y % de cilindros afectos en biopsia), ninguna de ellas alcanzó significación estadística, siendo el nivel de PSA t el más próximo a la misma. Conclusiones: La radioterapia externa representa una alternativa válida en el tratamiento del cáncer de próstata localmente avanzado con un tolerable índice de secundarismos. Debe emplearse en tratamiento combinado junto a la hormonoterapia. Parece que el empleo de unas dosis más altas de radiación, posible de manera segura gracias a la radioterapia conformada 3D, permiten alcanzar una mayor eficacia. Es probable que el indicador clínico con mayores implicaciones pronósticas sea el nivel de PSA total. Abstract in english Introduction and objectives: Locally advanced prostate cancer supposes a high risk condition of post-treatment progression due to the limit situation that represents. Our purpose was to analyze prognoses factors in function of progression probability after using a treatment with external source radi [...] otherapy on patients with this kind of tumors. Material and methods: We retrospectively reviewed a set of 128 patients submitted to pelvic staging limphadenectomy prior to accomplish an external radiotherapeutic treatment. We employed the Kaplan-Meier curves to study the probability of progression, logarithmic ranks test were used for detection of possible statistically significant differences and proportional risks Cox model was employed to study possible risk factors of progression (employing astro criteria). Results: 5 years freedom probability from progression was of 49,93%; in spite of appreciating important differences in the groups stratified by the predictive variables used (total PSA, gleason of pathological biopsy, clinical stage and % of cores affection on biopsy), none of them reached statistical meaning, being the level of total PSA the closest to it. Conclusions: The external radiotherapeuthic treatment represents a valid alternative in the treatment of locally advanced prostate cancer, with a tolerable index of secondaries. It must be used combined with hormonotherapy. It seems that the use of higher radiation doses, in a safer way thanks to 3D conformed radiotherapy, allows to improve the results. The most powerful clinical predictor of evolution must be the total PSA.

A., Barbagelata López; J.L., Ponce Díaz-Reixa; E., Romero Selas; F., Gómez Veiga; E., Fernández Rosado; M., González Martín.

2006-10-01

239

A prospective evaluation of real-time PCR assays for the detection of Orientia tsutsugamushi and Rickettsia spp. for early diagnosis of rickettsial infections during the acute phase of undifferentiated febrile illness.  

OpenAIRE

One hundred and eighty febrile patients were analyzed in a prospective evaluation of Orientia tsutsugamushi and Rickettsia spp. real-time polymerase chain reaction (PCR) assays for early diagnosis of rickettsial infections. By paired serology, 3.9% (7 of 180) and 6.1% (11 of 180) of patients were confirmed to have acute scrub or murine typhus, respectively. The PCR assays for the detection of O. tsutsugamushi and Rickettsia spp. had high specificity (99.4% [95% confidence interval (CI): 96.8-...

Watthanaworawit, W.; Turner, P.; Turner, C.; Tanganuchitcharnchai, A.; Richards, Al; Bourzac, Km; Blacksell, Sd; Nosten, F.

2013-01-01

240

Exposure and Risk Factors to Coxiella burnetii, Spotted Fever Group and Typhus Group Rickettsiae, and Bartonella henselae among Volunteer Blood Donors in Namibia  

Science.gov (United States)

Background The role of pathogen-mediated febrile illness in sub-Saharan Africa is receiving more attention, especially in Southern Africa where four countries (including Namibia) are actively working to eliminate malaria. With a high concentration of livestock and high rates of companion animal ownership, the influence of zoonotic bacterial diseases as causes of febrile illness in Namibia remains unknown. Methodology/Principal Findings The aim of the study was to evaluate exposure to Coxiella burnetii, spotted fever and typhus group rickettsiae, and Bartonella henselae using IFA and ELISA (IgG) in serum collected from 319 volunteer blood donors identified by the Blood Transfusion Service of Namibia (NAMBTS). Serum samples were linked to a basic questionnaire to identify possible risk factors. The majority of the participants (64.8%) had extensive exposure to rural areas or farms. Results indicated a C. burnetii prevalence of 26.1% (screening titre 1?16), and prevalence rates of 11.9% and 14.9% (screening titre 1?100) for spotted fever group and typhus group rickettsiae, respectively. There was a significant spatial association between C. burnetii exposure and place of residence in southern Namibia (P0.012), especially cattle (P>0.006), were also significantly associated with C. burnetii exposure. Males were significantly more likely than females to have been exposed to spotted fever (P<0.013) and typhus (P<0.011) group rickettsiae. Three (2.9%) samples were positive for B. henselae possibly indicating low levels of exposure to a pathogen never reported in Namibia. Conclusions/Significance These results indicate that Namibians are exposed to pathogenic fever-causing bacteria, most of which have flea or tick vectors/reservoirs. The epidemiology of febrile illnesses in Namibia needs further evaluation in order to develop comprehensive local diagnostic and treatment algorithms. PMID:25259959

Noden, Bruce H.; Tshavuka, Filippus I.; van der Colf, Berta E.; Chipare, Israel; Wilkinson, Rob

2014-01-01

241

Survey of canine tick-borne diseases in Lábrea, Brazilian Amazon: ‘accidental’ findings of Dirofilaria immitis infection / Pesquisa de agentes transmitidos por carrapatos em cães de Lábrea, Amazonas: achados “acidentais” de Dirofilaria immitis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Amostras de sangue foram coletadas de 99 cães domésticos de áreas urbana e rural do município de Lábrea, estado do Amazonas. Soros caninos foram testados pela técnica de imunofluorescência indireta contra Rickettsia spp., resultando em apenas 3,0% (1/33) e 7,6% (5/66) de cães soropositivos nas áreas [...] urbana e rural, respectivamente. DNA foi extraído do sangue canino e testado por diferentes protocolos da PCR para detecção de protozoários dos gêneros Babesia e Hepatozoon, e bactérias dos gêneros Rickettsia e Ehrlichia e da família Anaplasmataceae. Todas as amostras foram negativas nos protocolos de PCR para os gêneros Babesia, Hepatozoon, Ehrlichia e Rickettsia. Para Anaplasmataceae, 3% (1/33) e 39,4% (26/66) dos cães de áreas urbana e rural, respectivamente, geraram sequências de DNA 100% idênticas ao endosimbionte Wolbachia de Dirofilaria immitis. Posteriormente, as amostras foram testadas pela PCR para nematódeos filarídeos, resultando em 18,2% (6/33) e 57,6% (38/66) de amostras positivas nas áreas urbana e rural, respectivamente. Os produtos geraram sequências de DNA 100% idênticas a D. immitis. Em contraste com várias outras regiões do Brasil, infecções transmitidas por carrapatos foram raras em Lábrea. Por outro lado, as frequências de infecção por D. immitis estiveram entre as mais altas relatadas na América do Sul. Abstract in english Blood samples were collected from 99 domestic dogs from the urban and rural areas of the Lábrea municipality, state of Amazonas, Brazil. Canine serum samples were tested by immunofluorescence assay against Rickettsia spp., which revealed that only 3.0% (1/33) and 7.6% (5/66) of the dogs from urban a [...] nd rural areas, respectively, reacted positively to at least one Rickettsia species. DNA was extracted from canine blood and tested by a battery of PCR assays targeting protozoa of the genera Babesia and Hepatozoon, and bacteria of the genera Rickettsia and Ehrlichia and family Anaplasmataceae. All samples were negative in the PCR assays targeting the genera Babesia, Hepatozoon, Ehrlichia and Rickettsia. For Anaplasmataceae, 3% (1/33) and 39.4% (26/66) of the urban and rural dogs, respectively, yielded amplicons that generated DNA sequences 100% identical to the corresponding sequence of Wolbachia endosymbiont of Dirofilaria immitis. Because of these results, all canine DNA samples were further tested in a PCR assay targeting filarial nematodes, which was positive for 18.2% (6/33) and 57.6% (38/66) urban and rural dogs, respectively. Filarial-PCR products generated DNA sequences 100% identical to D. immitis. While tick-borne infections were rare in Lábrea, D. immitis infection rates were among the highest reported in South America.

Herbert Sousa, Soares; Luis Marcelo Aranha, Camargo; Solange Maria, Gennari; Marcelo Bahia, Labruna.

2014-12-01

242

Dose-response model of murine typhus (Rickettsia typhi: time post inoculation and host age dependency analysis  

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Full Text Available Abstract Background Rickettsia typhi (R. mooseri is the causative agent of murine typhus. It is one of the most widely distributed flea-borne diseases with a relatively mild febrile initial illness with six to 14 days of incubation period. The bacterium is gram negative and an obligate intracellular pathogen. The disease is transmitted to humans and vertebrate host through fleabites or via contact with infected feces. This paper develops dose-response models of different routes of exposure for typhus in rodents. Methods Data from published articles were analyzed using parametric dose-response relationship models. Dose-response relationships were fit to data using the method of maximum likelihood estimation (MLE. Results Dose-response models quantifying the effects of different ages of rats and time post inoculation in BALB/c mice were analyzed in the study. Both the adult rats (inoculated intradermally and newborn rats (inoculated subcutaneously were best fit by exponential models and both distributions could be described by a single dose-response relationship. The BALB/C mice inoculated subcutaneously were best fit by Beta-Poisson models. The time post inoculation analysis showed that there was a definite time and response relationship existed in this case. Conclusions Intradermally or subcutaneously inoculated rats (adult and newborn models suggest that less than 1 plaque-forming unit (PFU (1.33 to 0.38 in 95% confidence limits of the pathogen is enough to seroconvert 50% of the exposed population on average. For the BALB/c mouse time post inoculation model, an average dose of 0.28 plaque-forming units (PFU (0.75 to 0.11 in 95% confidence limits will seroconvert 50% of the exposed mice.

Tamrakar Sushil B

2012-03-01

243

por láser  

Directory of Open Access Journals (Sweden)

Full Text Available En el presente trabajo, la técnica Espectroscopia de plasmas producidos por láser (Laser-induced breakdown spectroscopy –LIBS– se aplicó a la determinación cuan- titativa de Na en agua natural dulce, de interés en agricultura para el estudio de la alcalinidad de aguas de regadío. Para efectuar el análisis, se prepararon soluciones con concentraciones conocidas del analito, se mezclaron con óxido de calcio y se compactaron en pastillas sólidas. Los plasmas se produjeron en aire a presión atmos- férica utilizando un láser pulsado Nd:YAG. Se construyó una curva de calibración y se calculó el límite de detección. Se analizaron muestras de agua natural y los resultados se compararon con los obtenidos mediante espectroscopia de absorción atómica. Se demostró la factibilidad del método para la determinación de Na en agua natural dulce.

Mayra Garcimu\\u00F1o

2013-01-01

244

Tratamiento adyuvante con quimioterapia y radioterapia luego de gastrectomía y disección ganglionar D2 en pacientes con cáncer gástrico en el Instituto Nacional de Enfermedades Neoplásicas, Lima, Perú / Adjuvant chemo radiotherapy after gastrectomy and D2 lymphadenectomy in patients with gastric cancer in the National Institute of Cancer, Lima, Peru  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Introducción: La quimio radioterapia adyuvante es una alternativa de tratamiento especialmente para pacientes con cirugías inferiores a D2. El estudio INT016 estableció la quimio radioterapia adyuvante como el cuidado estándar para adenocarcinoma resecado de alto riesgo del estómago en Estados Unido [...] s, sin embargo, se vio obstaculizado por la cirugía sub óptima. Existe controversia acerca de la eficacia de esta terapia adyuvante en pacientes sometidos a linfandenectomía D2. En nuestra institución la linfandenectomía D2 es la cirugía estándar para cáncer gástrico. Objetivo: Demostrar que la terapia adyuvante de quimio y radioterapia en pacientes con cáncer gástrico sometidos a gastrectomía y disección ganglionar D2 es efectiva. Materiales y métodos: Estudio retrospectivo de pacientes con adenocarcinoma gástrico estadío II a IV M0 quienes se sometieron a resección curativa en el Instituto Nacional de Enfermedades Neoplásicas, Lima-Perú en el periodo 2001 a 2006. El tratamiento estándar en la Institución es la linfandenectomía D2 y quimio radioterapia adyuvante de acuerdo al INT016. Las curvas de sobrevida fueron estimadas con el método Kaplan-Meier y comparadas con la prueba logrank. Resultados: 84 pacientes fueron incluidos 60,7% hombres y 39,3% mujeres. La edad media fue 49,5 años. Los estadios patológicos fueron T1-T2 (15,5%), T3-T4 (84,5%), N0-N1 (10,7%), N2-N3 (89,3%). Linfandenectomía D2 fue desarrollada en todos los pacientes. Encontramos una sobrevida libre de enfermedad a 3 años de 17% y una sobrevida global de 23,9%. Cuando se analiza por subgrupos, la sobrevida global en los grupos N1, N2 y N3 fueron 66,7%, 58,9% y 18,3%, respectivamente y la sobrevida libre de enfermedad fue 100%, 51,9% y 16,3%, respectivamente. Conclusiones: La quimio radioterapia adyuvante podría ser una alternativa para disminuir el riesgo de muerte y recaída a tres años principalmente en pacientes con ganglios positivos N1-N2, quienes se sometieron a resección curativa con linfandenectomía D2, pero la recurrencia fue más frecuente en ganglios positivos N3, la quimioterapia en este grupo de pacientes podría ser una alternativa para disminuir la tasa de recaída. Abstract in english Introduction: Adjuvant chemo radiotherapy is the standard treatment in Western countries in gastric cancer patients submitted to curative resection. INT0116 pivotal trial established adjuvant chemo radiation as the standard care for resected high risk adenocarcinoma of the stomach in US however was [...] hampered by suboptimal surgery. There is controversial data about efficacy of this adjuvant therapy in patients who have undergone D2 lymphadenectomy predominantly. In our hospital D2 lymphadenectomy is standard surgery for gastric cancer. Objective: To prove that chemo and radio therapy post gastrectomy and D2 linphadenectomy in patients’ with gastric cancer is effective. Material and methods: Retrospective study with gastric adenocarcinoma patients stage II to IV M0 who underwent curative resection at INEN (Instituto Nacional de Enfermedades Neoplasicas) Lima-Peru between 2001 and 2006. Standard treatment at institution is D2 lymphadenectomy. Chemo radiotherapy according to INT0116 was given like adjuvant therapy. Survival curves were calculated according to Kaplan-Meier method and compared with log-rank test. Results: 84 patients were included 60.7% male and 39.3% female. Mean age was 49.5 years old. The pathologic stages were T1-T2 (15.5%), T3-T4 (84.5%), N0-N1 (10.7%), N2-N3 (89.3%). D2 lymphadenectomy was performed in all patients. The 3-year DFS was 17% and 3-year overall survival was 23.9%. However when we analyzed by subgroups the overall survival, was in group N1 (66.7%) and in group N2 (58.9%) and N3 (18.3%) and 3 years DFS by subgroups were N1 (100%), N2 (51.9%) and N3 (16.3%). Conclusions: Adjuvant chemo radiotherapy decreased risk of death and relapse to three years mainly in patients with node positive N1-N2, who underwent curative

Paola, Montenegro; Lourdes, Flores; Shirley, Quintana; Luís, Casanova; Eloy, Ruíz; Jorge, León; Raúl, Mantilla.

2013-01-01

245

Rickettsiosis transmitidas por garrapatas en las Américas: avances clínicos y epidemiológicos, y retos en el diagnóstico / Tick-borne rickettsioses in the Americas: clinical and epidemiological advances, and diagnostic challenges  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Las rickettsiosis son entidades clínicas de tipo zoonótico, causadas por bacterias intracelulares estrictas de los géneros Rickettsia y Orientia, pertenecientes a la familia Rickettsiaceae. Su ecología está determinada por factores ambientales y la presencia de vectores específicos que condicionan e [...] l establecimiento y la epidemiología en diferentes regiones del mundo. En las Américas, durante el siglo XX, únicamente eran reconocidas tres de estas enfermedades: la fiebre manchada de las Montañas Rocosas, el tifus epidémico y el tifus endémico, Sin embargo, a partir del año 2000 se han descrito mas de 10 especies diferentes previamente desconocidas en este continente, tanto en artrópodos como en casos clínicos, hecho que permite clasificarlas como entidades clínicas emergentes y reemergentes. Dadas las manifestaciones clínicas de las enfermedades causadas por rickettsias, siendo la gran mayoría inespecíficas y, por lo mismo, compartidas con otras enfermedades infecciosas, especialmente virales y bacterianas, han sido enmarcadas entre los diagnósticos diferenciales del síndrome febril agudo, tanto en áreas urbanas como tropicales. En la actualidad, se cuenta con métodos diagnósticos directos e indirectos, que son útiles en la identificación del agente infeccioso, en este caso, causante de rickettsiosis. Abstract in english Rickettsioses are a group of zoonotic diseases caused by strict intracellular bacteria of the genus Rickettsia and Orientia which belong to the Rickettsiaceae family. Their ecology is influenced by environmental factors and the presence of specific vectors that determine the establishment and epidem [...] iology in different world regions. In America, during the 20 th century, only three of these diseases were recognized: Rocky Mountain spotted fever, epidemic typhus and endemic typhus. However, since 2000, more than 10 different species that had previously been unknown in this continent have been described, both in arthropods and in clinical cases, fact that classifies them as emerging and re-emerging diseases. Given the clinical manifestations of the diseases caused by rickettsias, being the majority unspecific and, therefore, shared with other infectious diseases, especially viral and bacterial, they have been framed within the differential diagnoses of acute febrile syndrome in urban and tropical areas. Nowadays, there are direct and indirect diagnostic methods, which are useful in the definition of the infectious agent, in this case, the cause of rickettsioses.

Marylin, Hidalgo; Álvaro A, Faccini-Martínez; Gustavo, Valbuena.

2013-09-01

246

Amputación interescapulotorácica por cromomicosis y carcinoma epidermoide / Amputation interscapulothracique pourchr4omomycose et6 carcinome épidermoide / Interscapulothoracic amputation by chromomycosis and epidermoid carcinoma  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Paciente del sexo masculino y blanco de 74 años de edad, con lesión dermatológica hiperpigmentada y verrucosa de más de 25 años de evolución en codo y antebrazo izquierdo; asimismo posee otra de piel en forma de coliflor y cuya evolución es reciente. Ambas presentaron diagnóstico histopatológico de [...] cromomicosis. El tratamiento inicial fue la exéresis con margen oncológico de la lesión en forma de coliflor y la electrofulguración, curetaje del resto de la lesión y tratamiento antimicótico. En un período de 5 meses el enfermo presenta evolución tórpida con toma del estado general y elefantiasis del miembro superior izquierdo hasta región supraclavicular que obliga a realizarle amputación interescapulotorácica por la técnica de Berger para mejorar la calidad de vida. El diagnóstico histopatológico de los paquetes ganglionares resecados mostró metástasis de un carcinoma epidermoide. Abstract in english The case of a 74-year-old white male patient with a hyperpigmented and verrucose dermatological injury of more than 25 years of evolution in his left elbow and forearm is reported. He also has another cauliflower-like skin injury of recent evolution. Both presented histopathological diagnosis of chr [...] omomycosis. The initial treatment was exeresis with oncological margin of the cauliflower-like injury and electrofulguration, curettage of the rest of the injury and antimycotic treatment. In 5 months, the patient had a torpid evolution with taking of the general state and elephantiasis of the upper left extremity to the supraclavicular region that led to the interscapulothoracic amputation by Berger’s technique to improve his quality of life. The histopathological diagnosis of the resected ganglionar packages showed metastasis of an epidermoid carcinoma.

Hiralio, Collazo Álvarez; Eridán, González Velázquez; Andrés G, Pardillo Morales; Stephen Yecc, Collazo Marín.

2001-12-01

247

Spotted fever from Rickettsia typhi in an older woman: a case report from a geographic area where it would not be expected.  

Science.gov (United States)

We describe the case of a 75-year-old woman presenting with spotted fever followed by acute renal failure and septic shock. The infection was caused by Rickettsia typhi, not reported in Calabria district (southern Italy) since World War II. The diagnosis of murine typhus was made 3 days after admission and was based solely on clinical criteria when her worsening condition required a prompt move to the intensive care unit. Therapy with tigecycline was then started immediately and the patient improved dramatically. The diagnosis of murine typhus was confirmed 10 days after admission by immunofluorescence assay. Our case is an example of how the diagnosis of murine typhus is challenging. However, in the case of a disease lacking specific symptoms, clinicians should never forget that, even in geographic areas considered free of flea-borne diseases, the components of the enzootic cycle are present and the diagnosis should never be underestimated. PMID:25111740

Luciani, Filippo; Cione, Erika; Corsonello, Andrea; Guido, Francesca; De Santis, Salvatore; Cannataro, Roberto; Perri, Mariarita; Caroleo, Maria Cristina; Cannataro, Anna Maria

2014-10-01

248

Detecção de Ehrlichia spp./Anaplasma spp., Rickettsia spp., Mycoplasma haemofelis e Leishmania infantum em felinos errantes e sua relação com a presença de retrovírus e com a sintomatologia manifestada  

OpenAIRE

ABSTRACT - Detection of Ehrlichia spp./Anaplasma spp., Rickettsia spp., Mycoplasma haemofelis and Leishmania infantum in stray cats, and its relation to the presence of retroviruses and the symptoms manifested - The prevalence of infectious diseases on cats is related with the density and the size of the population indwells. The objective of this work was to determinate the prevalence of the feline immunodeficiency virus (FIV), feline leukemia virus (FeLV), Ehrlichia spp./Anaplasma s...

Martins, Telma Simone Oliveira

2012-01-01

249

A prospective evaluation of real-time PCR assays for the detection of Orientia tsutsugamushi and Rickettsia spp. for early diagnosis of rickettsial infections during the acute phase of undifferentiated febrile illness.  

Science.gov (United States)

One hundred and eighty febrile patients were analyzed in a prospective evaluation of Orientia tsutsugamushi and Rickettsia spp. real-time polymerase chain reaction (PCR) assays for early diagnosis of rickettsial infections. By paired serology, 3.9% (7 of 180) and 6.1% (11 of 180) of patients were confirmed to have acute scrub or murine typhus, respectively. The PCR assays for the detection of O. tsutsugamushi and Rickettsia spp. had high specificity (99.4% [95% confidence interval (CI): 96.8-100] and 100% [95% CI: 97.8-100], respectively). The PCR results were also compared with immunoglobulin M (IgM) immunofluorescence assay (IFA) on acute sera. For O. tsutsugamushi, PCR sensitivity was twice that of acute specimen IgM IFA (28.6% versus 14.3%; McNemar's P = 0.3). For Rickettsia spp., PCR was four times as sensitive as acute specimen IgM IFA (36.4% versus 9.1%; P = 0.08), although this was not statistically significant. Whole blood and buffy coat, but not serum, were acceptable specimens for these PCRs. Further evaluation of these assays in a larger prospective study is warranted. PMID:23732256

Watthanaworawit, Wanitda; Turner, Paul; Turner, Claudia; Tanganuchitcharnchai, Ampai; Richards, Allen L; Bourzac, Kevin M; Blacksell, Stuart D; Nosten, François

2013-08-01

250

Primeiro caso de febre maculosa brasileira branda associada à artrite / First report of mild Brazilian spotted fever associated to arthritis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Descrevemos o primeiro caso brasileiro de Riquetsiose branda, agravada por monoartrite em joelho, em adulto jovem picado por carrapato na perna esquerda na região de Camburi, localizada no município de São Sebastião, sul da região costeira do estado de São Paulo, Mata Atlântica, Brasil. O paciente a [...] presentou uma escara de inoculação no local da picada do carrapato, associada ao aumento ganglionar em virilha esquerda, febre, poliartralgia, cefaleia e erupção macular. Vinte dias após o episódio da picada de carrapato, o paciente apresentou monoartrite em joelho direito. O diagnóstico de Riquetsiose branda foi estabelecido pela análise imunológica sequencial em amostras de soro e líquido sinovial, tendo sido empregada a técnica de imunofluorescência (IF) indireta para anticorpos reativos contra Rickettsia parkeri e Rickettsia rickettsii. A Riquetsiose branda é uma zoonose emergente, que deve ser investigada pelos médicos, incluindo reumatologistas, em pacientes que apresentem erupção macular, febre e, eventualmente, artrite, após visita ao sul da região costeira da Mata Atlântica no Brasil. Abstract in english We describe the first Brazilian case of mild Rickettsiosis, complicated by knee monoarthritis, in young adult bitten by a tick on his left leg in Camburi zone, located in São Sebastião municipality, southern coastal region of the State of São Paulo, in the Atlantic rainforest region, Brazil. The pat [...] ient developed inoculation eschar at the tick bite site associated with enlarged lymph nodes in the left groin, fever, polyarthralgia, headache and macular rash. Twenty days after tick bite episode, he displayed monoarthritis in his right knee. The diagnosis of mild Rickettsiosis was established by sequential immunological analysis in serum and synovial fluid, using the indirect immunofluorescence (IF) assay for antibodies reactive with Rickettsia parkeri and Rickettsia rickettsii. The mild Rickettsiosis is an emerging zoonosis, that must be investigated by physicians, including rheumatologists, in patients that present macular rash, fever and eventually arthritis, after visiting the southern coastal Atlantic rainforest region in Brazil.

Virgínia Lucia Nazario, Bonoldi; Roberta Gonçalves, Marangoni; Giancarla, Gauditano; Jonas, Moraes-Filho; Marcelo Bahia, Labruna; Natalino Hajime, Yoshinari.

2014-06-01

251

Linfadenopatía por silicona en una paciente con prótesis mamarias / Silicone-induced lymphadenopathy in a patient suffering from mammary prosthesis: a case report  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Se presenta el caso de una mujer de 47 años, con adenopatías axilares izquierdas, sin antecedentes médicos y a quien se le habían colocado implantes mamarios 8 años antes con fines estéticos. Se realizó resección ganglionar y la patología reportó células gigantes multinucleadas tipo cuerpo extraño, [...] con abundantes vacuolas citoplasmáticas y linfocitos polimorfonucleares con lo que se estableció el diagnóstico de adenopatía por silicona y se procedió a la remoción y cambio de la prótesis. La biopsia por aspiración con aguja fina (BACAF) de los ganglios linfáticos que drenan el sitio donde se localiza la prótesis puede hacer el diagnóstico de linfadenopatía por silicona y a la vez permite descartar la presencia de un proceso maligno que pudiera coincidir en algunas pacientes. Abstract in english This is a case report of a 47-year old woman having no antecedents of disease, but a surgical history of silicone breast-implants 8 years beforehand. The patient’s complaint concerned the left axillary lymph nodes. A ganglion resection was done and fine needle aspiration biopsy (FNAB) revealed giant [...] multinucleated cells having abundant cytoplasmatic vacuoles and polymorphonuclear lymphocytes leading to a diagnosis of silicon-induced adenopathy. The prosthesis was then removed and changed. Lymph node FNAB is able to diagnose silicone lymphadenopathy and excludes malignant neoplasm.

Germán, García-Soto; Jorge, Castaño; Ronald, Smith-Meneses.

2006-06-01

252

Linfadenopatía por silicona en una paciente con prótesis mamarias Silicone-induced lymphadenopathy in a patient suffering from mammary prosthesis: a case report  

Directory of Open Access Journals (Sweden)

Full Text Available Se presenta el caso de una mujer de 47 años, con adenopatías axilares izquierdas, sin antecedentes médicos y a quien se le habían colocado implantes mamarios 8 años antes con fines estéticos. Se realizó resección ganglionar y la patología reportó células gigantes multinucleadas tipo cuerpo extraño, con abundantes vacuolas citoplasmáticas y linfocitos polimorfonucleares con lo que se estableció el diagnóstico de adenopatía por silicona y se procedió a la remoción y cambio de la prótesis. La biopsia por aspiración con aguja fina (BACAF de los ganglios linfáticos que drenan el sitio donde se localiza la prótesis puede hacer el diagnóstico de linfadenopatía por silicona y a la vez permite descartar la presencia de un proceso maligno que pudiera coincidir en algunas pacientes.This is a case report of a 47-year old woman having no antecedents of disease, but a surgical history of silicone breast-implants 8 years beforehand. The patient’s complaint concerned the left axillary lymph nodes. A ganglion resection was done and fine needle aspiration biopsy (FNAB revealed giant multinucleated cells having abundant cytoplasmatic vacuoles and polymorphonuclear lymphocytes leading to a diagnosis of silicon-induced adenopathy. The prosthesis was then removed and changed. Lymph node FNAB is able to diagnose silicone lymphadenopathy and excludes malignant neoplasm.

Germán García-Soto

2006-06-01

253

Characterization of a Rickettsia rickettsii DNA fragment analogous to the fir A-ORF17-lpxA region of Escherichia coli.  

Science.gov (United States)

The firA and lpxA genes, as well as an ORF coding for a putative 16-kDa protein of unknown function, have been identified and characterized in the obligate intracellular bacterium. Rickettsia rickettsii. This is the first description of these genes, which code for enzymes involved in the biosynthesis of lipid A, in a species outside of the Enterobacteriaceae. The deduced amino acid (aa) sequences of FirA, ORF16 and LpxA of R. rickettsii, when compared to their Escherichia coli analogs, exhibited 35, 44 and 41% aa identity, respectively. In addition, the order of genes in R. rickettsii, firA-ORF16-lpxA, was identical to that found in E. coli; however, the spacing between the rickettsial genes was greater. Interestingly, the R. rickettsii FirA and LpxA deduced proteins retain an unusual hexapeptide repeat pattern found in E. coli and Salmonella typhimurium FirA/Ssc and E. coli LpxA, as well as other acyltransferases, providing additional support for the importance of this structure. PMID:8125326

Shaw, E I; Wood, D O

1994-03-11

254

Tuberculosis ganglionar cervical simulando una enfermedad de Kikuchi: Reporte de un caso y revisión de la literatura / Cervical lymph node tuberculosis simulating Kikuchi's disease: A case report and review of the literature  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish La linfadenitis cervical tuberculosa es la manifestación más común de tuberculosis en cabeza y cuello. La enfermedad de Kikuchi es una enfermedad rara que consiste en una linfadenitis necrotizante histocitiaria. La presentación clínica en ambas entidades suele consistir en una o más masas cervicales [...] dolorosas, que pueden ser duras o fluctuantes. Los hallazgos por imagen no permiten distinguir de manera absoluta la linfadenitis tuberculosa de la enfermedad de Kikuchi. Por lo tanto es imprescindible para interpretar los hallazgos por imagen no sólo conocer el origen demográfico del paciente, sino su estado inmunológico con respecto a la tuberculosis y el estudio histopatológico y microbiológico de las adenopatías. Abstract in english Tuberculous cervical lymphadenitis is the most common manifestation of tuberculosis in the head and neck. Kikuchi's disease is a rare disease that is histocitiaria necrotizing lymphadenitis. The clinical presentation in both entities is usually a painful neck masses or more, which can be hard or flu [...] ctuating. The imaging findings do not distinguish in absolute tuberculous lymphadenitis Kikuchi's disease. Therefore it is essential to interpret the imaging findings not only know the origin of the patient population, but their immune status with regard to Tuberculosis and histopathological and microbiological lymphadenopathy.

Lisbeth, Valoyes G; Iván, García D; Amadeo, Muntané S; Paloma, Mora M; Lucía, Aja R.

255

Cuantificación de ADN como factor pronóstico en un caso de Carcinoma de células Acinares de parótida diagnosticado por PAAF / DNA quantification as prognostic factor in a case of Acinar Cell Carcinoma of the parotid gland, diagnosed by FNA  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Presentamos el caso de un varón de 43 años de edad que consulta por una tumoración preauricular derecha dolorosa a la palpación que, según refiere, tiene más de tres años de evolución. Se realiza punción aspiración con aguja fina que informa de compatible con carcinoma de células acinares, por lo qu [...] e se realiza cuantificación de ADN por citometría de imagen. Los parámetros biológicos estudiados (ploidía, fase S, células por encima de 5c) indican que se trata de una lesión de bajo grado de malignidad. Con estos datos se practica parotidectomía total conservadora del facial, sin vaciamiento ganglionar. El paciente se encuentra, un año después, asintomático y libre de signos tumorales residuales. Abstract in english Hereby we present a case of a 43-years-old male who complained of a three years history preauricular painful mass. Fine needle aspiration cytology was performed, diagnosing of compatible with acinar cell carcinoma, thus DNA quantification by image cytometry was carried out. Biological parameters stu [...] died (ploidy, S-phase, 5-c exceeding rate) showed that it is a low grade of malignancy lesion. Total parotidectomy conservative of facial nerve was recommended, without regional lymphadenectomy. Patient remains, one year later, asymptomatic and free of disease.

Javier, Azúa Romeo; Juan Carlos, Sánchez Garnica; Javier, Azúa Blanco; Mayte, Tovar Lázaro.

2005-10-01

256

Factores morfológicos asociados al pronóstico de pacientes operados por cáncer gástrico incipiente / Pathological features associated with survival in early gastric cancer  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: El cáncer gástrico incipiente (CGI) es aquel que compromete la mucosa o submucosa gástrica independientemente del compromiso ganglionar linfático, estimándose su prevalencia en Chile inferior al 20%o. El objetivo de este estudio es determinar prevalencia de CGI y asociación de variable [...] s biode-mográficas y morfológicas con la supervivencia (SV) de pacientes resecados por CGI. Material y Método: Estudio de cohorte retrospectiva. Se estudiaron variables biodemográficas y morfológicas de 106 pacientes resecados por CGI entre 1986-2007. Se aplicó estadística descriptiva y analítica; confección de curvas de SV, y finalmente se aplicaron modelos de regresión logística para realizar ajuste, calcular odds ratio y sus respectivos intervalos de confianza de 95%. Resultados: 15% correspondió a CGI. La mediana de edad fue 63 años y el 60% correspondió a género masculino con una SV global a 5 años de 91%. Se observaron diferencias estadísticas significativas entre tumores mucosos y submucosos en cuanto a la localización tumoral y compromiso linfonodal junto con presentarse el compromiso nodal más frecuentemente en tumores > 35mm poco diferenciados y difusos de Lauren. El análisis multivariado identificó como factores asociados a la SV: tamaño tumoral, grado de diferenciación histológica en su variedad poco diferenciado, tipo difuso de Lauren y compromiso ganglionar linfático. Conclusiones: Se verificó una prevalencia de CGI de 15%, los que resecados presentan SV de 91% a 5 años. El compromiso linfonodal es un factor asociado a la SV; y además, se relaciona con tamaño tumoral, tipo histológico según Lauren, grado de diferenciación histológico y nivel de infiltración. Abstract in english Background: Early gastric cancer corresponds to those tumors that only involve mucosa and submuco-sa. It is associated with a high survival rate. Aim: To determine pathological factors associated with survival in early gastric cancer. Material and Methods: Analysis of pathological records of 106 pat [...] ients, with a median age of 63 years (60%> males), subjected to a gastrectomy for early gastric cancer. Follow up was performed according to data in the clinical records and death certificates obtained at the Chilean National Death Registry. Results: Five years global survival of patients was 91%o. Lymph node involvement was more common among tumors bigger than 35 mm, with a low degree of differentiation and among those tumors classified as diffuse according to Lauren. Survival was significantly lower for bigger tumors, those with of a low degree of differentiation, diffuse tumors according to Lauren and those with lymph node involvement. Conclusions: Early gastric cancer has a high five years survival. Bigger tumors, those with a low degree of differentiation and those with lymph node involvement are associated with lower survival rates.

ÓSCAR, TAPIA E; PATRICIA, GARCÍA M; CARLOS, MANTEROLA D; MIGUEL, VILLASECA H; JUAN CARLOS, ARAYA O; JUAN CARLOS, ROA S.

2011-04-01

257

Depletion of gamma interferon and tumor necrosis factor alpha in mice with Rickettsia conorii-infected endothelium: impairment of rickettsicidal nitric oxide production resulting in fatal, overwhelming rickettsial disease.  

OpenAIRE

C3H/HeN mice infected intravenously with a dose of Rickettsia conorii (Malish 7 strain) that is sublethal for immunocompetent animals (1.1 x 10(3) PFU) developed disseminated infection of endothelial cells of the brain, lungs, heart, liver, kidney, testis, and testicular adnexa. In R. conorii-infected mice depleted of gamma interferon (IFN-gamma) and/or tumor necrosis factor alpha (TNF-alpha) by intravenous administration of neutralizing monoclonal antibodies on days 0, 2, and 4, the mortalit...

Feng, H. M.; Popov, V. L.; Walker, D. H.

1994-01-01

258

Loop-mediated isothermal amplification for Rickettsia typhi (the causal agent of murine typhus): problems with diagnosis at the limit of detection.  

Science.gov (United States)

Murine typhus is a flea-borne disease of worldwide distribution caused by Rickettsia typhi. Although treatment with tetracycline antibiotics is effective, treatment is often misguided or delayed due to diagnostic difficulties. As the gold standard immunofluorescence assay is imperfect, we aimed to develop and evaluate a loop-mediated isothermal amplification (LAMP) assay. LAMP assays have the potential to fulfill the WHO ASSURED criteria (affordable, sensitive, specific, user friendly, robust and rapid, equipment free, deliverable to those who need them) for diagnostic methodologies, as they can detect pathogen-derived nucleic acid with low technical expenditure. The LAMP assay was developed using samples of bacterial isolates (n=41), buffy coat specimens from R. typhi PCR-positive Lao patients (n=42), and diverse negative controls (n=47). The method was then evaluated prospectively using consecutive patients with suspected scrub typhus or murine typhus (n=266). The limit of detection was ?40 DNA copies/LAMP reaction, with an analytical sensitivity of <10 DNA copies/reaction based on isolate dilutions. Despite these low cutoffs, the clinical sensitivity was disappointing, with 48% (95% confidence interval [95% CI], 32.5 to 62.7%) (specificity, 100% [95% CI, 100 to 100%]) in the developmental phase and 33% (95% CI, 9.2 to 56.8%) (specificity, 98.5% [95% CI, 97.0% to 100%]) in the prospective study. This low diagnostic accuracy was attributed to low patient R. typhi bacterial loads (median, 210 DNA copies/ml blood; interquartile range, 130 to 500). PCR-positive but LAMP-negative samples demonstrated significantly lower bacterial loads than LAMP-positive samples. Our findings highlight the diagnostic challenges for diseases with low pathogen burdens and emphasize the need to integrate pathogen biology with improved template production for assay development strategies. PMID:24371248

Dittrich, Sabine; Castonguay-Vanier, Josée; Moore, Catrin E; Thongyoo, Narongchai; Newton, Paul N; Paris, Daniel H

2014-03-01

259

Síndrome de Frey por submaxilectomía y tratamiento con toxina botulínica Frey syndrome secondary to submaxillectomy and botulinic treatment  

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Full Text Available El síndrome de Frey (SF se caracteriza por rubor, calor y sudación en la región maseterina y geniana durante las comidas. El SF se observa en el 20-60% de los pacientes sometidos a parotidectomía. Presentamos un caso con SF secundario a la ablación de la glándula submaxilar en un varón de 30 años de edad, sin antecedentes patológicos, que consultó en el año 2000 por adenopatía cervical izquierda. La misma fue biopsiada y la anatomía patológica informó carcinoma medular de tiroides. El paciente presentaba múltiples metástasis hepáticas y pulmonares, motivo por el cual recibió tratamiento quimioterápico con cisplatino plus doxorrubicina (seis ciclos con respuesta completa, finalizando dicho esquema en marzo del 2002. En julio del 2002 se realizó tiroidectomía total más vaciamiento ganglionar izquierdo con resección de la glándula submaxilar. Durante la cirugía se lesionó la rama mentoniana del nervio cervicofacial, rama del VII par craneal. En septiembre del 2004 consultó por episodios de sudación durante las comidas en la región submaxilar izquierda. Se realizó prueba de Minor y luego se procedió a aplicar toxina botulínica tipo A en la región afectada, a razón de 2.5 UI por punto (cm², a un total de 17 puntos. El control a los 21 días demostró más de un 95% de efectividad en la resolución del SF. El efecto duró un año, y una segunda reinfiltración mostró similar respuesta. No hallamos descripciones anteriores de SF en región submaxilar; se presenta su tratamiento satisfactorio con toxina botulínica tipo A.A case of Frey syndrome (FS secondary to submaxillar gland exeresis is presented and the results of the treatment with botulinum toxin (BTX type A. FS is a condition of sweating cheek and preauricular area during mealtime as a sequel detected in about 20-60% of patients after parotidectomy. The clinical symptoms include swelling, flushing and hyperhidrosis. The treatment choice for this condition is intracutaneous injection of BTX type A which blocks acetylcholine release at the sweat glands. A 30-year-old man, with thyroid medullar carcinoma diagnosed in 2002 received 6 cicles of cisplatin plus doxorubicin previous to the thyroidectomy with anterolateral neck dissection. During surgery the left ramus marginalis mandibulae was damaged. Two years later the patient referred sweating in submaxillar region during meals. CT scan demonstrated the absence of left submaxillar gland. Minor's test disclosed the affected area and BTX type A was injected (2.5 U/cm²/17 points. A twenty-one-day control showed a 95% reduction of the affected skin area. Persistent efficacy was observed up to one year followup time when he was reinjected. The FS, also known as "gustatory hyperhidrosis", was probably first reported by M. Duphenix in 1757. Lucja Frey considered its physiopathology as a disorder of both sympathetic and parasympathetic innervation. In our case the FS was caused by a misdirected regeneration of postganglionic parasympathetic nerve fibers that arrised from the nervus lingualis rami ganglionares of the nervus trigeminus. After nerve injury the colinergic parasympathetic fibers seek out colinergic receptors -sympathetic receptors of the skin- innervating sweat glands and small skin vessels. All previous cases were located at masseter region post-parotidectomy. We have not found any description of FS in the submaxillary region. The self-assessed efficacy of the treatment with a hyperhidrosis disease severity scale revealed a very satisfied patient at 20 months follow-up after being injected twice with BTX type A.

Alejandra Baéz

2007-10-01

260

Síndrome de Frey por submaxilectomía y tratamiento con toxina botulínica / Frey syndrome secondary to submaxillectomy and botulinic treatment  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish El síndrome de Frey (SF) se caracteriza por rubor, calor y sudación en la región maseterina y geniana durante las comidas. El SF se observa en el 20-60% de los pacientes sometidos a parotidectomía. Presentamos un caso con SF secundario a la ablación de la glándula submaxilar en un varón de 30 años d [...] e edad, sin antecedentes patológicos, que consultó en el año 2000 por adenopatía cervical izquierda. La misma fue biopsiada y la anatomía patológica informó carcinoma medular de tiroides. El paciente presentaba múltiples metástasis hepáticas y pulmonares, motivo por el cual recibió tratamiento quimioterápico con cisplatino plus doxorrubicina (seis ciclos) con respuesta completa, finalizando dicho esquema en marzo del 2002. En julio del 2002 se realizó tiroidectomía total más vaciamiento ganglionar izquierdo con resección de la glándula submaxilar. Durante la cirugía se lesionó la rama mentoniana del nervio cervicofacial, rama del VII par craneal. En septiembre del 2004 consultó por episodios de sudación durante las comidas en la región submaxilar izquierda. Se realizó prueba de Minor y luego se procedió a aplicar toxina botulínica tipo A en la región afectada, a razón de 2.5 UI por punto (cm²), a un total de 17 puntos. El control a los 21 días demostró más de un 95% de efectividad en la resolución del SF. El efecto duró un año, y una segunda reinfiltración mostró similar respuesta. No hallamos descripciones anteriores de SF en región submaxilar; se presenta su tratamiento satisfactorio con toxina botulínica tipo A. Abstract in english A case of Frey syndrome (FS) secondary to submaxillar gland exeresis is presented and the results of the treatment with botulinum toxin (BTX) type A. FS is a condition of sweating cheek and preauricular area during mealtime as a sequel detected in about 20-60% of patients after parotidectomy. The cl [...] inical symptoms include swelling, flushing and hyperhidrosis. The treatment choice for this condition is intracutaneous injection of BTX type A which blocks acetylcholine release at the sweat glands. A 30-year-old man, with thyroid medullar carcinoma diagnosed in 2002 received 6 cicles of cisplatin plus doxorubicin previous to the thyroidectomy with anterolateral neck dissection. During surgery the left ramus marginalis mandibulae was damaged. Two years later the patient referred sweating in submaxillar region during meals. CT scan demonstrated the absence of left submaxillar gland. Minor's test disclosed the affected area and BTX type A was injected (2.5 U/cm²/17 points). A twenty-one-day control showed a 95% reduction of the affected skin area. Persistent efficacy was observed up to one year followup time when he was reinjected. The FS, also known as "gustatory hyperhidrosis", was probably first reported by M. Duphenix in 1757. Lucja Frey considered its physiopathology as a disorder of both sympathetic and parasympathetic innervation. In our case the FS was caused by a misdirected regeneration of postganglionic parasympathetic nerve fibers that arrised from the nervus lingualis rami ganglionares of the nervus trigeminus. After nerve injury the colinergic parasympathetic fibers seek out colinergic receptors -sympathetic receptors of the skin- innervating sweat glands and small skin vessels. All previous cases were located at masseter region post-parotidectomy. We have not found any description of FS in the submaxillary region. The self-assessed efficacy of the treatment with a hyperhidrosis disease severity scale revealed a very satisfied patient at 20 months follow-up after being injected twice with BTX type A.

Alejandra, Baéz; Julieta, Paleari; María Nöel, Durán; Tamara, Rudy; Inés, Califano; Nicolás, Barbosa; Ignacio, Casas Parera.

2007-10-01

261

Trombocitopenia induzida por heparina  

OpenAIRE

O objetivo deste relato é fazer uma revisão a fim de obter informações atualizadas sobre trombocitopenia induzida por heparina, suas manifestações clínicas, seu diagnóstico e seu manejo terapêutico. Após, concluímos que a trombocitopenia induzida por heparina é uma complicação comum em pacientes submetidos a tratamento com heparina, indiferentemente da doença de base. Complicações trombóticas potencialmente fatais têm sido descritas. Por essa razão, se houver suspeita de t...

Longhi Fernanda; Laks Dani; Kalil Nelson G.N.

2001-01-01

262

por medio de soldadura  

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Full Text Available El desgaste es un fenómeno de degradación de los diferentes materiales por efectos de abrasión, impacto, fatiga, erosión y corrosión entre otros. Este fenómeno acarrea grandes perdidas económicas por deterioro de elementos de máquinas y equipo industrial, asimismo una altísima cuota en impacto ambiental. Para contrarrestar los efectos nocivos generados por desgaste, surge la reconstrucción y mantenimiento de materiales por soldadura. Se tiene un molino vertical ATOX 32.5 para molienda de caliza con un juego de 6 placas borde de mesa, se hicieron aplicaciones de placas con revestimientos de soldadura con carburos de cromo y carburos complejos en las zonas más críticas.

Alberto Zapata Meneses

2007-01-01

263

(phoenicopterus roseus por intoxicación aguda por plomo  

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Full Text Available La presencia de plomo en el medio ambiente es un hecho indiscutible. Ello es objeto de gran interés sanitario pues repercute tanto en la salud de los animales como en la del ser humano. El plumbismo es una intoxicación que puede afectar a cualquier especie animal. En el ámbito de la fauna silvestre se asocia principalmente a la actividad cinegética en los humedales y a la ingestión de plomo metálico por parte de las aves que frecuentan estos ecosistemas. En España cada año mueren decenas de miles de aves acuáticas como consecuencia de la ingesta de perdigones de plomo. En los individuos capturados enfermos se pueden observar signos clínicos, mientras que a partir de muestras de cadáveres se obtienen datos sobre niveles tisulares de plomo. Sin embargo la evaluación de lesiones anatomopatológicas en animales de vida silvestre es difícil de realizar por la dificultad de encontrar animales recién muertos o afectados de un cuadro agudo mortal. La reproducción de esta patología en animales de experimentación se suele realizar en cisnes, palomas o patos, pero no en flamencos, por lo que no es usual disponer de datos histológicos en esta especie. En este trabajo presentamos la clínica, analítica y estudio microscópico de diferentes tejidos de un flamenco común (Phoenicopterus roseus muerto por plumbismo.

D Romero

2007-01-01

264

Valoración del análisis de la pieza operatoria en el cáncer gástrico por el cirujano Concordance between the surgical piece observation by the surgeon and final pathological report for gastric cancer  

Directory of Open Access Journals (Sweden)

Full Text Available Introducción: La disección de la pieza operatoria (PO en forma sistemática en la cirugía por cáncer gástrico (CG permite al cirujano obtener información de los bordes, compromiso linfonodal y estandarizar. Objetivos: 1 Determinar la concordancia de los hallazgos de la PO disecada por el cirujano y el informe patológico final; 2 Determinar el porcentaje de retracción del estómago; 3 Mostrar una forma de registrar la PO en el CG. Material y Método: Estudio prospectivo de los pacientes operados con intención curativa desde septiembre de 2009 hasta mayo de 2010. Se etapificó con TNM de 2010. De la PO se registró el tamaño del estómago por curvatura menor, límite oral (LO, límite caudal (LC, profundidad, aspecto macroscópico, diámetro tumoral, grupos ganglionares comprometidos y cantidad de ganglios comprometidos. Se compararon estas variables con el informe patológico. El análisis de concordancia se realizó con el test de Kappa y el test de Lin. Resultados: Fueron estudiados 48 pacientes, 74% hombres, la edad promedio fue 63,7 años (DE 9,8, 59,5% fueron gastrectomías totales. El promedio de ganglios disecados fue 30,18 (DE 11,8. Se obtiene una buena concordancia para la profundidad del tumor (Kappa = 0,64, aspecto macroscópico (Kappa = 0,69 y tamaño tumoral (Lin = 0,84. Y una mala concordancia para el compromiso ganglionar (Kappa = 0,21. El porcentaje de retracción del largo por curvatura menor fue 24,3%, del LO fue 30,1%, del LC fue 22%. Conclusión: La concordancia de la PO es buena en la profundidad, aspecto macroscópico del tumor y diámetro tumoral, pero es mala en el compromiso ganglionar. Se determina que la retracción del estómago es mayor al 24%.Background: The systematic dissection of the surgical piece, performed by the surgeon during surgical treatment of gastric cancer, gives information about borders and lymph node involvement. Aim: To determine the concordance between the findings of the surgeon during initial dissection and the final pathological report. Material and Methods: Prospective study of 48 patients aged 64 ± 10 years (74% males subjected to curative surgery for gastric cancer. Patients were staged according to 2010 TNM classification. Stomach size from the lesser curvature, oral and caudal limits, macroscopic aspect, tumor diameter and lymph node involvement were determined by the surgeon observing the surgical piece. The concordance of this observation with the final pathological report was assessed. Results: Fifty nine percent of patients were subjected to a total gastrectomy and there was a mean of 30 lymph nodes excised. There was a good concordance between surgeon observation and final pathological report for tumor depth (Kappa = 0.64, macroscopic aspect (Kappa = 0.69 and tumor size (Lin = 0.84. There was a bad concordance for lymph node involvement (Kappa = 0.21. The percentage of retraction of lesser curvature length was 24%, 30% for oral and 22% for caudal limits. Conclusions: There is a good concordance between surgeon observation and pathological report for macroscopic aspect, tumor size and depth but the concordance for lymph node involvement is bad.

MARCO CERONI V

2011-08-01

265

Estudio Comparativo de Disección Ganglionar D1 y D2 para el Cáncer Gástrico Avanzado en el Hospital Rebagliati / Comparative Study of D1 and D2 Ganglionic Dissection in Advanced GAstric Cancer at Rebagliati Hospital  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish La gastrectomía con linfadenectomía D2 es la cirugía estándar en la mayoría de centros hospitalarios del Perú debido a que la técnica quirúrgica japonesa ha mostrado los mejores resultados en todo el mundo. Sin embargo nunca se ha realizado un estudio comparando los resultados de linfadenectomía D2 [...] con D1 en nuestro país. El presente estudio de tipo cohorte retrospectivo compara la linfadenectomía D2 versus D1 en el tratamiento quirúrgico del cáncer gástrico. 316 pacientes fueron sometidos a cirugía por cáncer gástrico entre Enero del 2000 y Diciembre del 2002 en el Hospital Nacional Rebagliati y se seleccionaron en forma aleatoria un total de 100 pacientes, 50 de ellos fueron sometidos a linfadenectomía D1 y otros 50 a linfadenectomía D2 respectivamente. La mortalidad fue de 8% para D1 y 4 % para D2 (p: NS), mientras que la morbilidad fue de 24% para D1 y 12% para D2 (p: NS). La sobrevida a 3 años fue de 36% para D1 y 56% para D2 estableciendo una diferencia estadísticamente significativa (p: 0.04) Se recomienda la gastrectomía con linfadenectomía D2 como tratamiento quirúrgico de elección para el cáncer gástrico avanzado. Abstract in english Gastrectomy with D2 lymphadenectomy is the standard surgical procedure in most hospitals in Peru due to the fact that this Japanese surgical technique has proven to provide the best results in the world. However, no comparative study of the results of D1 and D2 lymphadenectomies has ever been perfor [...] med in our country. This retrospective cohort study compares D2 lymphadenectomy versus D1 in surgical treatment of gastric cancer. Between January 2000 and December 2002, 316 patients underwent surgery at the Rebagliati National Hospital and a total of 100 patients were randomly selected, 50 of which underwent a D1 lymphadenectomy and 50 a D2 lymphadenectomy. Mortality rates were 8% for D1 lymphadenectomy and 4% for D2, whereas morbidity rates were 24% for D1 and 12% for D2. Survival after 3 years was 36% for D1 and 56% for D2 thus establishing a statistically significant difference (p = 0.04). We recommend gastrectomy with D2 lymphadenectomy as the surgical treatment of choice for advanced gastric cancer.

Consuelo, Cornejo; Michel, Portanova.

2006-10-01

266

Pecoma pélvico del ligamento ancho con metástasis ganglionares: presentación de un caso y revisión de la literatura / Pelvic PEcoma of the broad ligament with lymph node metastasis: Case report and bibliographic review  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Objetivos: Describimos y documentamos el primer caso de PEComa publicado en España según la base de datos Pub Med. Y revisamos la bibliografía existente sobre este tipo de tumores. Métodos/Resultados: Se Trata de una paciente de 39 años con un tumor pélvico de 9 cms. descubierto en una revisión gine [...] cológica rutinaria. El TAC muestra múltiples adenopatías periaórticas que se extienden hasta vena renal izquierda El diagnóstico de PEComa se obtiene por punción con aguja gruesa. Se realiza exéresis del tumor junto a anexo izquierdo y linfadenectomía aortoiliaca. El resultado patológico confirma el diagnóstico tras la inmunohistoquímica con actina de músculo liso y HMB-45. No recibió tratamiento adyuvante. Tras un año de seguimiento la paciente no muestra en el TAC signos de recidiva. Se realiza una amplia búsqueda bibliográfica en Pub Med encontrando 73 referencias a este tipo de tumor cuyas conclusiones se exponen en este articulo. Conclusiones: El tumor de celulas epitelioides perivasculares, PEComa, es una neoplasia mesenquimal de muy baja incidencia y con malignidad incierta. Tumores muy raros: el angiomiolipoma epitelioide, la linfangioleiomiomatosis, el linfangiomioma, el tumor miomelanocitico del ligamento falciforme, el tumor pulmonar de celulas claras "de azucar" y su variante extrapulmonar; son antiguas descripciones de lo que ahora es una única entidad tumoral denominada PEComa. Aun no de ha descrito la célula que en tejidos normales da origen a este tumor. Afectando principalmente a mujeres en edad media, pueden encontrarse en cualquier lugar anatómico. Su tratamiento es quirúrgico aunque se desconoce el papel de la linfadenectomía y del tratamiento adyuvantes. Abstract in english Objective: We describe and document the first case of PEcoma published in Spain following the PubMed database. We review the bibliography about these tumors. Methods/Results: 39-year-old female patient with a 9 cm pelvic tumor discovered in a routine gynecologic review. CT scan showed multiple peria [...] ortic adenopathies extending up to the left renal vein. The diagnosis of PEcoma was obtained by needle biopsy. Tumor excision including left annex and aortoiliac lymphadenectomy were performed. Pathology confirmed the diagnosis after immunohistochemical study with smooth muscle actin and HMB-45. No adjuvant treatment was given. After one year of follow-up the patient does not show signs of recurrence of the CT scan. A PubMed search was performed finding 73 references about this kind of tumor the conclusions of which are exposed in this article. Conclusions: Perivascular epithelioid cell tumor, PEcoma, is a very low incidence mesenchymal neoplasia with uncertain malignancy. Very rare tumors such as epithelioid angiomyolipoma, lymphangioleiomyomatosis, lymphangiomyoma, myelomelanocytic tumor of the falciform ligament, "sugar" clear cell lung tumor and its extrapulmonary variant are all descriptions of what is now a unique tumoral entity named PEcoma. It has not been described yet the normal tissue cell giving origin to this tumor. It affects mainly middle age women, and may appear in any anatomical site. Treatment is surgical, although the role of lymphadenectomy and adjuvant treatment is unknown.

Raul, Tobalina Bonis; Tomás, Pérez Domínguez; Fernanda, Relea Calatayud; Enrique, Cazenave Jiménez; M. A., Piris; Nemesio, Jiménez López-Lucendo.

2008-08-01

267

Meningococcemia complicada por miocardite  

OpenAIRE

Homem de 26 anos com meningococcemia (meningite), complicada por miocardite (disfunção ventricular e isquemia miocárdica), requereu tratamento para insuficiência cardíaca e evolui com regressão da disfunção miocárdica, seis meses após o quadro infeccioso.

Fº, Albanesi Francisco Manes; Rocha Ricardo Mourilhe; Barros Rui Alves

1998-01-01

268

NEURECTOMIA PRESACRA POR LAPAROSCOPIA  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Se presenta nuestra experiencia con neurectomía presacra por laparoscopia. De 100 pacientes intervenidas por vía laparoscópica por dolor pélvico crónico en 10 se realizó neurectomía presacra como intervención única o junto a exéresis de endometriosis. En 9 de las 10 pacientes se logró mejoría con pe [...] ríodos de seguimiento prolongado y sin complicaciones. La neurectomía presacra por laparoscopia es una técnica eficaz en el control del dolor pélvico crónico central severo refractario a tratamiento médico Abstract in english We present our experience with laparoscopic presacral neurectomy (LPSN). We describe the technique and results. One hundred patients with chronic pelvic pain were operated. In ten of these LPSN was done for severe chronic pelvic pain. Ninety percent of success without complications was the final res [...] ult. LPSN proved to be efficacious procedure in patients who do not respond to medical therapy

David, Escobar P.; Carmen Luz, Alvarado S.; Juan Carlos, Barros; Mónica, Molina R..

269

Factores asociados al pronóstico de pacientes operados por cáncer gástrico avanzado / Pathological factors associated with survival in advanced gastric cancer  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: El estudio de la pieza operatoria de pacientes resecados por cáncer gástrico (CG) ha permitido identificar variables anatomo-patológicas con valor pronóstico en la supervivencia (SV) y recurrencia de estos pacientes, siendo el compromiso ganglionar linfático y nivel de infiltración tum [...] oral, los factores más relevantes identificados. El objetivo de este estudio es determinar asociación entre variables clínicas y morfológicas con la SV de pacientes resecados por CG avanzado (CGA). Material y Método: Estudio de cohorte retrospectiva. Se estudiaron variables clínicas y morfológicas de 299 pacientes operados por CGA entre enero de 1986-diciembre de 2001. Los datos fueron obtenidos desde la Unidad de Anatomía Patológica del Hospital Hernán Henríquez Aravena de Temuco. Se aplicó estadística descriptiva y analítica; confección de curvas de supervivencia, y finalmente se aplicaron modelos de regresión logística para realizar ajuste, calcular odds ratios y sus respectivos intervalos de confianza de 95%o. Resultados: La cohorte tuvo una mediana de edad de 63 años y el 68% de ella correspondió al género masculino. Con una mediana de seguimiento de 21 meses (1 a 206), se observó una SV actuarial global a 5 y 10 años de 39% y 34% respectivamente. En el análisis bivariado, se verificó asociación con la SV en: etapa tumoral macroscópica, localization y tamaño tumoral, tipo según Bormann, nivel de infiltración, grado de diferenciación histológico, tipo histológico según Lauren, Ming y Nakamura, estado ganglionar linfático (N), estadio TNM y resultado de la resección realizada. Conclusiones: Las variables mencionadas deben ser cuidadosamente evaluadas al momento de decidir terapias en pacientes con CGA. Abstract in english Background: The degree of tumor infiltration and lymph node involvement are the most relevant pathological features to determine prognosis of advanced gastric cancer. Aim: To determine the association between clinical and pathological features of advanced gastric cancer and patient survival. Materia [...] l and Methods: The pathological records of patients with advanced gastric cancer subjected to gastrectomy and lymph node excision between 1986 and 2007 were analyzed. Follow up was performed according to data in the clinical records and death certificates obtained at the Chilean National Death Registry. The main outcome analyzed was survival after surgery. Results: The records of 299 patients aged 62 ± 11 years (68% males), were analyzed. Mean follow up ranged from 1 to 206 months. Five and 10 years actuarial survival was 39 and 34%, respectively. The pathological predictors of survival were microscopic tumor stage, tumor size and location, Bormann classification, infiltration level, degree of differentiation, pathological type of tumor according to Lauren, Ming y Nakamura, lymph node involvement and the absence of residual tumor after surgical excision. Conclusions: The pathological study of the surgical piece in advanced gastric cancer has important prognostic implications.

ÓSCAR, TAPIA E; JUAN CARLOS, ROA S; CARLOS, MANTEROLA D; VALENTINA, PUGA A; MIGUEL, VILLASECA H; JUAN CARLOS, ARAYA O.

2011-04-01

270

superficial por soldadura automática  

Directory of Open Access Journals (Sweden)

Full Text Available En el siguiente trabajo se evalúa el aumento de la resistencia al desgaste abrasivo de las superficies de trabajo de aperos para la labranza de suelos, luego de aplicar el recargue superficial por el método de soldadura automática por arco sumergida (SAAS, utilizando para ello un fundente cerámico obtenido a partir de la reconstitución de la escoria del propio proceso. Se realizan mediciones de macrodureza, microdureza y resitencia al desgaste, se determina además el índice de desgate en condiciones de campo, utilizando medios reales de labranza de suelos.

Elvis L\\u00F3pez B.

2007-01-01

271

Comparação entre diversos antígenos para o diagnóstico de Anaplasma marginale por ELISA / Comparison between several antigens for diagnosis of Anaplasma marginale by ELISA  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Anaplasmose bovina é uma doença com grande importância nas regiões tropicais e subtropicais do mundo por determinar perdas econômicas devido à mortalidade e redução da produtividade. É causada por Anaplasma marginale, uma riquétsia intraeritrocítica obrigatória cujo controle requer, além de uma vaci [...] na eficiente, uma acurada identificação de bovinos cronicamente infectados. Apesar de existirem atualmente diversos métodos de diagnóstico dessa riquétsia, os métodos sorológicos, em particular o ensaio de imunoadsorção enzimática-ELISAs, são os mais utilizados devido à sua versatilidade e praticidade. No entanto, devido ao grande número de antígenos disponíveis, atualmente torna-se necessária uma avaliação para definir quais antígenos apresentam um melhor desempenho no diagnóstico da anaplasmose. Soros de bovinos positivos e negativos para A. marginale por PCR, e soros de animais provenientes do Brasil e Costa Rica, foram testados em ELISAs baseados em MSP1a, MSP2 e MSP5 recombinantes, um pool das três proteínas recombinantes, e antígeno de lisado de corpúsculos iniciais da riquétsia (CI). Utilizando soro de bovinos positivos para A. marginale por PCR, uma maior sensibilidade foi observada no ELISA CI. No entanto, uma maior especificidade, com soro de bovinos negativos a PCR, foi observada com os ELISAs recombinantes. O porcentual de bovinos positivos do Brasil e Costa Rica foi maior com ELISA CI. Razões para essas diferenças são discutidas. Abstract in english Bovine anaplasmosis is a major disease in tropical and subtropical regions of the world by determine economical loss due mortality and productive reduction. The disease is caused by Anaplasma marginale, an intraerythrocytic rickettsia whose control requires, besides an efficient vaccine, the accurat [...] e identification of chronically infected cattle. Although the existence of diverse methods of diagnosis of this rickettsia, the serological methods, in particular the enzyme immunosorbent assays (ELISAs), are the most used due to its versatility and practice. However, due to the high number of antigens currently available, an evaluation becomes necessary to define which antigens present the better performance in the diagnosis of anaplasmosis. Sera from cattle positive or negative to A. marginale by PCR, and sera from cattle proceeding from Brazil and Costa Rica, were tested by ELISAs based in recombinant MSP1a, MSP2, and MSP5, a pool of the three recombinant proteins, and initial body lisate antigen (CI). Using sera from A. marginale positive cattle by PCR, the highest sensitivity was shown by CI ELISA. Nevertheless, the highest specificity, with sera from negative cattle by PCR, was shown by recombinants ELISAs. The percentiles of positive cattle from Brazil and Costa Rica were higher with CI ELISA. Reasons for such differences were discussed.

Carlos A.N., Ramos; Flábio R., Araújo; Ingrid I.F., Souza; Daniel S., Guedes Jr; Renato H.M., Oliveira; Thaís A., Farias; Jaqueline B., Oliveira; Leucio C., Alves; Maria A.G., Faustino.

2010-01-01

272

Dermatitis canina por Malassezia  

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Full Text Available La dermatitis por Malassezia en perros en generalmente una complicación a procesos alérgicos, seborreicos, presencia de pliegues, etc. aunque también se han descrito otras presentaciones, como la hipersensibilidad contra esta levadura. La demostración de la presencia de Malassesia pachydermatis se hace de manera sencilla mediante citología. El tratamiento de esta dermatitis puede ser tópico u oral.

Rejas López, Juan

2008-05-01

273

Intoxicación por Paraquat  

Scientific Electronic Library Online (English)

Full Text Available SciELO Costa Rica | Language: Spanish Abstract in spanish El paraquat es un herbicida bipiridílico que actúa por contacto, se presenta en forma líquida en concentraciones del 20% para uso agrícola. Su nombre químico es eI 1-1´-dimetil-4-4- bipiridilo, con el nombre comercial de Gramoxone. La intoxicación por paraquat tiene una tasa de mortalidad elevada. L [...] a intoxicación grave se caracteriza por la afectación de múltiples órganos, principalmente los pulmones, los riñones y el hígado. El pulmón es el órgano diana en la intoxicación por paraquat y la insuficiencia respiratoria con fibrosis pulmonar aguda es la causa más común de muerte. Abstract in english Paraquat (1-1´-dimetil-4-4-bipyridylium dichloride) is still widely used in Costa Rica as a contact herbicide and is marketed as a 20% aqueous solution (Gramoxone) .Paraquat poisoning carries a high mortality rate. Severe paraquat poisoning is characterized by multiple-organ involvement, mainly the [...] lungs, kidneys, liver. The lung is a major target organ in paraquat poisoning, and respiratory failure from acute pulmonary fibrosis is the most common cause of death.

Glenda, Viales López.

2014-12-01

274

tirados por tractores  

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Full Text Available En el presente trabajo se brinda la metodología de realización y evaluación de los principales indicadores energéticos de los agregados de transporte agrícola, formados por tractores y remolques con el uso de la tensometría como herramienta para la toma de los datos experimentales de campo.

Ciro E. Iglesias Coronel

2006-01-01

275

Intoxicación por paraquat  

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Full Text Available En el periodo comprendido de 1996 a 1999, se revisaron los expedientes clínicos de enfermos intoxicados por biperidilos (Paraquat, para determinar las principales manifestaciones, complicaciones, tratamiento y pronóstico del tóxico. Se realizó un estudio retrospectivo, transversal, descriptivo y observacional, en el área de Medicina Interna del Hospital General ?Dr. Gustavo A. Rovirosa Pérez.? Se estudiaron 18 pacientes con intoxicación por paraquat, dos se excluyeron para fines de evolución pero no de mortalidad por datos incompletos en el expediente. De los l6 pacientes estudiados con intoxicación por biperidilos (Paraquat, el sexo masculino ocupó el mayor número, l3 hombres (81.25% y 3 mujeres, con un rango de edad de 15 a 74 años. El producto químico comercial más utilizado fue el Gramoxone, siendo la principal vía de intoxicación la oral, en 93.75% con cantidades variables entre 3 y 750cc. Las principales manifestaciones al ingreso se incluyeron en la fase 1, que consiste en inflamación, edema y ulceración de la capa mucosa de tubo digestivo, y fase 2 con daño hepático y renal. Las principales complicaciones fueron a nivel digestivo, renal, hepático y pulmonar. El tratamiento incluyó medidas básicas de urgencias, generales y, en algunos casos especiales diálisis peritoneal aguda. Conclusiones. Los factores predisponentes fueron intento suicida por depresión reactiva breve y alcoholismo. La complicación pulmonar fue determinante en la mortalidad, debido a que el tejido pulmonar adquiere más concentración del tóxico que otros tejidos. A pesar de su alta toxicidad, la mortalidad fue del 56%. El tratamiento debe ser vigoroso e iniciado tan rápido como sea posible.

Norma A. Hern\\u00E1ndez Hern\\u00E1ndez

2000-01-01

276

Vectors of rickettsiae in Africa.  

Science.gov (United States)

Vector-borne diseases are caused by parasites, bacteria, or viruses transmitted by the bites of hematophagous arthropods. In Africa, there has been a recent emergence of new diseases and the re-emergence of existing diseases, usually with changes in disease epidemiology (e.g., geographical distribution, prevalence, and pathogenicity). In Africa, rickettsioses are recognized as important emerging vector-borne infections in humans. Rickettsial diseases are transmitted by different types of arthropods, ticks, fleas, lice, and mites. This review will examine the roles of these different arthropod vectors and their geographical distributions. PMID:23168053

Bitam, Idir

2012-12-01

277

Ascitis quilosa por maltrato  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish La ascitis quilosa es la acumulación de líquido quiloso en la cavidad peritoneal. Puede ser primaria (defectos congénitos) o secundaria (obstrucción, traumatismos, etc.). Es nuestro objetivo poner en conocimiento una forma poco común de presentación de síndrome de maltrato infantil. Se presenta una [...] niña de un año de edad, que ingresó a nuestro hospital por presentar un cuadro de maltrato infantil con distensión abdominal. Se realizaron estudios de laboratorio, diagnóstico por imágenes y una paracentesis de abdomen que arrojó como resultado un líquido compatible con quilo. Con el diagnóstico de ascitis quilosa se realizaron estudios complementarios que confirmaron un traumatismo abdominal por síndrome de maltrato infantil. La paciente evolucionó favorablemente con ayuno y nutrición parenteral por un período de un mes, y posteriormente con realimentación con dieta hipograsa. Es de hacer notar que la revisión bibliográfica demostró que de 41 casos de ascitis quilosa en edad pediátrica, 10% fueron secundarios a maltrato. Si tomamos el rango de edades de 2 meses a 2 años este porcentaje aumenta a 44%. Abstract in english Chylous ascites is the accumulation of chylous fluid in the peritoneal cavity. It can be primary (congenital) or secondary (acquired), due to trauma or obstruction. We present a child who developed a chylous ascites resulting from child abuse. This one year old female infant was admitted to our hosp [...] ital with suspicion of child abuse syndrome and abdominal distention. Laboratory tests, radiographic examination and paracenthesis showed the presence of chylous fluid in the abdomen. Complementary exams confirmed the diagnosis of abdominal trauma secondary to child abuse. The outcome was good; the girl underwent fasting and parenteral nutrition for one month, and refeeding with a low fat diet. In this case, paracenthesis has shown to be useful for the differential diagnosis of ascites of unknown origin, as well as treatment with parenteral nutrition and fasting.

Jorge, Hauri; Hector, Pelusso; Daniela, Perli; José, Pujol; Luis, Miraglia; Roque, Malvasso.

2004-04-01

278

Intoxicación aguda por plaguicidas  

OpenAIRE

OBJETIVO: Describir la epidemiología de la intoxicación aguda por plaguicidas (IAP) en el Hospital General O'Horán de Mérida, Yucatán, México. MATERIAL Y MÉTODOS: Se revisaron los expedientes de 33 pacientes superscript three 13 años, tratados en una unidad de cuidados intensivos (UCI), entre 1994 y 1998. Se recopilaron variables demográficas y clínicas relevantes, y se aplicó estadística descriptiva. RESULTADOS: En la población estudiada predominó el ...

Durán-Nah Jaime Jesús; Collí-Quintal Julián

2000-01-01

279

La lucha por Guayaquil  

Directory of Open Access Journals (Sweden)

Full Text Available En el contexto del referendo constitucional del 2008 en Ecuador, este texto analiza la disputa entre Rafael Correa, presidente del Gobierno ecuatoriano y principal líder de Alianza País, y el alcalde la ciudad más grande del país, Jaime Nebot. Disputa que es examinada a partir de tres ejes: la lucha contra el neoliberalismo, la construcción de un proyecto estatal nacional y la búsqueda por una por una hegemonía política de Alianza País. Se profundiza en las nociones de autonomía y globalización que sirvieron de eje al discurso del alcalde, frente al modelo de autonomía propuesto por el Gobierno.In the context of Ecuador's 2008 Constitutional Referendum, this text analyses the dispute between Rafael Correa –the President of the Ecuadorian government and the figurehead of Alianza País– and the mayor of the largest city in the country, Jaime Nebot. The argument will be developed along three fronts: the battle against neoliberalism, the construction of a national state project, and the search for the political hegemony of Alianza País. The article examines the concepts of autonomy and globalization that were central to Nebot's discourse and contrasts them with the autonomous model put forward by the government.

Felipe Burbano de Lara

2009-01-01

280

colonización por glomus intraradices  

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Full Text Available El presente estudio es el primer reporte sobre la inducción de raíces transformadas por Agrobacterium rhizogenes en Agave salmiana Otto, así como del establecimiento de un hongo micorrízico en las mismas. Para lograr lo anterior se inocularon plantas germinadas in vitro con diferentes concentraciones de bacteria y de acetosiringona en varios sitios (hoja, tallo y raíz. El tiempo de cocultivo en oscuridad fue de 6 d. Las raíces transformadas se presentaron a los 25 d después de la inoculación. La mayor eficiencia de transformación resultó de la inoculación al tallo con 1x109 bacterias mL-1 y 200 µM de acetosiringona, condiciones en las que se obtuvo 63 % de raíces transformadas. La naturaleza transgénica de las raíces generadas se verificó mediante un ensayo histoquímico para detectar actividad de GUS y los transgenes se amplificaron en muestras de ADN de raíz a través de PCR. Se detectó actividad de GUS en 80 % de los tejidos probados, mientras que los genes rolB y nptII se amplificaron en 60 % de las muestras de ADN analizadas por PCR. Se demostró la capacidad de Glomus intraradices para colonizar in vitro las raíces transformadas de A. salmiana, con una eficiencia de colonización de 70 %. Se logró la recuperación de esporas hijas, con un promedio de 300 esporas hijas por cultivo, a los 6 meses de iniciada la inoculación.

Guillermo Rodr\\u00EDguez Hern\\u00E1ndez

2007-01-01

281

Brazo robótico controlado por electromiografía  

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Full Text Available En el presente trabajo se describe la construcción de un brazo robótico controlado por señales Electromiográficas EMG, las cuales son tomadas por los electrodos que son conectados al brazo del paciente. Esas señales, son controladas por unos sensores superficiales de EMG. Los biopotenciales que pasan por los músculos del brazo son controlados por el Dispositivo microcontrolado marca Arduino, en el cual dependiendo de la diferencia de potencial que circule por el músculo va activar los servomotores que controlan el brazo robótico. Se utilizan 4 servomotores y sensores de EMG para darle 8 grados de libertad al brazo robótico.

Javier Villamizar Pinz\\u00F3n

2012-01-01

282

Comportamiento de la visión de color en pacientes sospechosos de glaucoma y glaucomatosos como daño precoz de las células ganglionares de la retina / Colour vision of patients suspected of and diagnosed with glaucoma as an early damage of ganglionary cells of the retina  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Se realizó un estudio descriptivo y analítico en 60 pacientes (sospechosos de glaucoma y con diagnóstico de glaucoma crónico simple estadio inicial) que concurrieron a la consulta de Oftalmología del Hospital Militar Central "Dr. Carlos J. Finlay" en el período comprendido entre los años 2003 y 2006 [...] . La muestra se conformó con 30 pacientes con sospecha de glaucoma y 30 con diagnóstico de glaucoma que cumplieron los requisitos de inclusión y exclusión. Los primeros 30 pacientes de cada grupo que fueron atendidos durante ese período fueron los seleccionados. Se les aplicaron diferentes test para evaluar la visión de colores, como daño precoz de las células ganglionares de la retina. Se consideró sospechoso de glaucoma todo paciente que tuviese al menos tres de los más importantes factores de riesgos (HTO, edad mayor de 40 años, raza negra y antecedentes familiares de primer grado que padezcan la enfermedad). Se diagnosticó como glaucomatoso aquel paciente que presentó síntomas y signos de la enfermedad y alteraciones incipientes del campo visual (aumento mancha ciega, escalón nasal o escotoma Seidel). También se utilizó en el estudio una muestra de 31 sujetos supuestamente sanos de la investigación realizada en el Hospital "Hermanos Ameijeiras" bajo las mismas condiciones de exploración como grupo control -que se tituló "Estudio de la visión cromática en una población sana". El método de evaluación de los pacientes consistió en un examen inicial oftalmológico donde se recogieron sus datos generales paciente, síntomas y signos clínicos hallados, así como factores de riesgo de la enfermedad. Se constató que los sujetos sanos no presentaban alteraciones en relación con los colores y que los sospechosos y glaucomatosos sí las presentaron en un índice significativo Abstract in english A descriptive analytical study of 60 patients suspected of glaucoma and diagnosed with simple chronic glaucoma at initial stage, who went to the Ophtalmology service of “Dr. Carlos J. Finlay” Military Hospital in the period from 2003 to 2006. The sample covered 30 patients suspected of and 30 patien [...] ts diagnosed with glaucoma that fulfilled inclusion and exclusion requirements. The first 30 patients of each group seen in that period were selected. Several tests were apppied to evaluate colour vision, as an early damage in ganglionary cells of the retina. Every patient having at least three important risk factors (high blood pressure, age over 40 years, black race and family history of first degree relatives suffering the dissease) was considered suspect of having glaucoma. The patients were diagnosed as glaucomatous when they presented with symptoms and signs of the disease and incipient alterations of the visual field (increase of blind stain, nasal step or Seidel scotoma). This study also used a sample of 31 supposedly healthy subjects, who had participated as control group under the same exploratory conditions in a prior research study at “Hermanos Ameijeiras” hospital. The title of that research was “Study of chromatic vision in a healthy population”. The evaluation method consisted of an initial ophthalmological exam where general data, clinical symptoms and signs as well as risk factors were collected. It was observed that healthy subjects did not present alterations in relation to colours and that individuals suspected of and diagnosed with glaucoma did have a significant index

José Alberto, Cabrera Martínez; Jorge, Martínez Ribalta; Melba, Márquez Fernández; Abel, Cabrera Martínez.

2007-06-01

283

Las imágenes por venir  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: Spanish Abstract in portuguese A partir de uma análise das conceições e modos de ensino e aprendizagem que se desprendem da analogia proposta pelo término alfabetizão, o artigo empreende uma crítica das atuais condições de transmissão do saber nas carreiras de Cinema e televisão, Design de Imagem e Som, e Design Audiovisual, disc [...] utindo o cariz deshistorizante de seus conteúdos, como assim também a primazia concedida ao código cinematográfico. A análise da enunciação, por sua vez, leva a um proponho sobre as implicancias políticas da construção de subjetividade que supõe a habilitação profissional, para finalmente propor uma idéia diferente de comunicação audiovisual a partir da noção de escritura. Abstract in spanish A partir de un análisis de las concepciones y modos de enseñanza y aprendizaje que se desprenden de la analogía propuesta por el término alfabetización, el artículo emprende una crítica de las actuales condiciones de transmisión del saber en las carreras de Cine y televisión, Diseño de imagen y soni [...] do y Diseño audiovisual, discutiendo el cariz deshistorizante de sus contenidos, como así también la primacía concedida al código cinematográfico. El análisis de la enunciación, por su parte, lleva a un planteo sobre las implicancias políticas de la construcción de subjetividad que supone la habilitación profesional, para finalmente proponer una idea distinta de comunicación audiovisual a partir de la noción de escritura. Abstract in english The article undertakes a critic of the present conditions of knowledge transmission in the careers of Cinema and Television, Sound and Image Design and Audiovisual Design, from an analysis of the conceptions and ways of teaching and learning that come off the analogy proposed by the term alphabetiza [...] tion. On the other hand, the analysis of enunciation, takes to a raise on the political implicancies about the construction of subjectivity in the professional qualification. Finally, the article offers a different idea of audiovisual communication after the notion of writing.

Hugo, Salas.

2012-03-01

284

Gestión por proyectos  

Directory of Open Access Journals (Sweden)

Full Text Available Se trata la Gestión de Proyectos por las técnicas de Dirección Integrada de Proyectos  o Project management, donde se dan algunas de las  definiciones de Gestión por Proyectos, así como su importancia y necesidad de trabajar por estas técnicas, no solo para los programas y Proyectos priorizados sino también en los proyectos  de desarrollo empresarial.Una buena gestión de proyectos requiere una inversión inicial de tiempo y esfuerzo y es necesario que toda la organización esté dispuesta a apoyarla de manera disciplinada. Que rompa la inercia de trabajo desorganizado e improvisado y procure apegarse, de manera responsable, al uso de la metodología de proyectos.La solución de problemas a partir de proyectos, genera una cultura de trabajo diferente a lo que habitualmente se ha  acostumbrado, surgiendo una figura nueva para liderar la solución del problema planteado: el jefe del proyecto. Dicho en otros términos, el proyecto se presenta como una entidad autónoma, se gobierna a partir de su propio diseño y depende solamente de su presupuesto.La administración de proyectos garantiza que se:v  Resuelven problemas más rápidamente.v  Evitan desperdicios y caos al no trabajar en áreas que están fuera del alcance del proyecto.v  Enfoca en resolver riesgos futuros antes de que los problemas ocurran.v  Orienta a manejar las expectativas y la comunicación entre clientes, colaboradores y grupos de interés de manera más efectiva.v  Busca crear productos de más alta calidad desde el primer momento.v  Termine en tiempo los proyectosv  Utilice el presupuesto de forma eficiente 

Ana Rosa Hernández Rodríguez

2010-10-01

285

Intoxicación por gases Gas poisoning  

OpenAIRE

La intoxicación por gases en nuestro medio es un problema importante debido a su alta incidencia. En el caso concreto de la intoxicación por monóxido de carbono, es la principal causa de muerte por intoxicación involuntaria en nuestro medio, muchas veces coexistiendo con una intoxicación por cianuro. Ambas intoxicaciones pueden ser de carácter grave, basándose su diagnóstico en la mera sospecha del médico. Además, su importancia radica en que ambas intoxicaciones tienen un tratamien...

Santiago, I.

2003-01-01

286

Intoxicación aguda por heroína  

Directory of Open Access Journals (Sweden)

Full Text Available La intoxicación por opiáceos se presenta casi siempre como consecuencia de una sobredosis accidental de un derivado de éstos, usualmente la heroína por inyección. Otras formas de intoxicación, a las que no somos ajenos en nuestro medio, son los llamados correos de drogas, "body packers" o mulas, utilizados en el tráfico internacional de transporte de drogas. En estos casos la droga es generalmente tragada, envuelta en condones, que pueden contener heroína o cocaína. Su ruptura puede conducir a toxicidad severa y letal. Los antecedente de un viaje reciente en avión en un paciente con cuadro clínico de intoxicación, pueden justificar la indicación de una radiografía de abdomen como método diagnóstico e incluso la cirugía para limpieza intestinal como parte de su abordaje terapéutico.Most of opioid related deaths for overdose are accidental and are primarily due to intravenous heroin use. Other forms of poisoning may occur in the so called body packers ("mulas", who are used in international drug transportation. These patients generally swallow small quantities of wrapped cocaine or heroin in an attempt to smuggle the drugs across international borders. Their breakage can lead to severe and lethal toxicity. The history of a recent a flight in a patient with a clinical syndrome of poisoning, should justify the indication of abdominal x-rays for diagnosis and also surgery for "intestinal cleaning" as part of his therapeutic approach.

Guillermo Porras-Morales

2004-03-01

287

Intoxicación aguda por heroína  

Scientific Electronic Library Online (English)

Full Text Available SciELO Costa Rica | Language: Spanish Abstract in spanish La intoxicación por opiáceos se presenta casi siempre como consecuencia de una sobredosis accidental de un derivado de éstos, usualmente la heroína por inyección. Otras formas de intoxicación, a las que no somos ajenos en nuestro medio, son los llamados correos de drogas, "body packers" o mulas, uti [...] lizados en el tráfico internacional de transporte de drogas. En estos casos la droga es generalmente tragada, envuelta en condones, que pueden contener heroína o cocaína. Su ruptura puede conducir a toxicidad severa y letal. Los antecedente de un viaje reciente en avión en un paciente con cuadro clínico de intoxicación, pueden justificar la indicación de una radiografía de abdomen como método diagnóstico e incluso la cirugía para limpieza intestinal como parte de su abordaje terapéutico. Abstract in english Most of opioid related deaths for overdose are accidental and are primarily due to intravenous heroin use. Other forms of poisoning may occur in the so called body packers ("mulas"), who are used in international drug transportation. These patients generally swallow small quantities of wrapped cocai [...] ne or heroin in an attempt to smuggle the drugs across international borders. Their breakage can lead to severe and lethal toxicity. The history of a recent a flight in a patient with a clinical syndrome of poisoning, should justify the indication of abdominal x-rays for diagnosis and also surgery for "intestinal cleaning" as part of his therapeutic approach.

Guillermo, Porras-Morales.

2004-03-01

288

revelada por marcadores RAPD  

Directory of Open Access Journals (Sweden)

Full Text Available El manzano (Malus domestica Borkh. es uno de los frutales de clima templado de mayor importancia en México, con 62 000 ha cultivadas en 2003, de las cuales alrededor de 700 se encuentran establecidas en el estado de Querétaro. La anarquía en la introducción de variedades cultivadas e híbridos por parte de los productores y las dependencias de gobierno, ha propiciado el desconocimiento de la identidad algunos de éstos, además, se ignora si los materiales introducidos se encuentran injertados sobre portainjertos clonales resistentes al pulgón lanígero (Eriosoma lanigerum, plaga importante en la región. Con el fin de evaluar la diversidad genética del manzano, se colectaron 65 muestras que incluyen variedades cultivadas, materiales criollos, híbridos y portainjertos de manzano y se analizaron por medio de marcadores moleculares tipo RAPD (ADN polimórfico amplificado al azar. Se seleccionaron 10 oligonucleótidos para el análisis del ADN. Se amplificaron 113 bandas polimórficas y 29 monomórficas. El análisis de conglomerados mostró que la mayor disimilitud entre genotipos fue de 0.71. Las muestras se separaron en dos conglomerados. El primero incluye casi todas las muestras relacionadas con la variedad `Golden Delicious´, mientras que el segundo grupo incluye la mayoría de las muestras correspondientes a manzanas rojas y portainjertos clonales. Los resultados indican que la diversidad genética del manzano en la región es importante y que el método RAPD permite agrupar genotipos con características comunes, lo que facilitará la identificación genética de variedades.

Azucena del Carmen Gonz\\u00E1lez-Horta

2005-01-01

289

Portable exhausters POR-004 SKID B, POR-005 SKID C, POR-006 SKID D storage plan  

International Nuclear Information System (INIS)

This document provides a storage plan for portable exhausters POR-004 SKID B, POR-005 SKID C, AND POR-006 SKID D. The exhausters will be stored until they are needed by the TWRS (Tank Waste Remediation Systems) Saltwell Pumping Program. The storage plan provides criteria for portable exhauster storage, periodic inspections during storage, and retrieval from storage

290

¿Por qué historia política?  

Directory of Open Access Journals (Sweden)

Full Text Available Es el estudio de la Historia Politica en México, la reflexión a que nos invita la autora, considerando que en los aiios setentas y ochentas, hay una crisis de la Historia respecto a otras disciplinas sociales, al considerársele como un "auxiliar" de Cstas, y no como la Ciencia Social que en realidad es. Sólo existían dos posibles interpretaciones de la historia: el marxismo y la escuela de los Annales, limitando asi las posibilidades de análisis amplios y profundos en la Historia Política, además de que ésta se consideraba irrelevante y desprestigiada. Por lo tanto, es hoy en día una necesidad imperiosa para la historiografia mexicana, el abordar temas que no se han analizado más que someramente, tales como los poderes ejecutivo y judicial, la participación de las secretarias de Estado, etc., proporcionándole a la Historia Política, la importancia que tiene dentro de las ramas de la Historia.

Josefina Mac Gregor

1999-01-01

291

An Investigation of Bartonella spp., Rickettsia typhi, and Seoul Hantavirus in Rats (Rattus spp.) from an Inner-City Neighborhood of Vancouver, Canada: Is Pathogen Presence a Reflection of Global and Local Rat Population Structure?  

Science.gov (United States)

Urban Norway and black rats (Rattus norvegicus and Rattus rattus) are reservoirs for variety of zoonotic pathogens. Many of these pathogens, including Rickettsia typhi, Bartonella spp., and Seoul hantavirus (SEOV), are thought to be endemic in rat populations worldwide; however, past field research has found these organisms to be absent in certain rat populations. Rats (Rattus spp.) from an inner city neighborhood of Vancouver, Canada, were tested for exposure to and/or infection with SEOV and R. typhi (using serology and PCR), as well as Bartonella spp. (using culture and sequencing). Approximately 25% of 404 rats tested were infected with Bartonella tribocorum, which demonstrated significant geographic clustering within the study area. Infection was associated with both season and sexual maturity. Seroreactivity against R. typhi and SEOV was observed in 0.36% and 1.45% of 553 rats tested, respectively, although PCR screening results for these pathogens were negative, suggesting that they are not endemic in the study population. Overall, these results suggest that the geographic distribution of rat-associated zoonoses, including R. typhi, SEOV, and Bartonella spp., is less ubiquitous than previously appreciated, and is likely dependent on patterns of dispersion and establishment of the rat reservoir host. Further study on global and local Rattus spp. population structures may help to elucidate the ecology of zoonotic organisms in these species. PMID:25629777

Himsworth, Chelsea G; Bai, Ying; Kosoy, Michael Y; Wood, Heidi; DiBernardo, Antonia; Lindsay, Robbin; Bidulka, Julie; Tang, Patrick; Jardine, Claire; Patrick, David

2015-01-01

292

Vagotomía transtorácica por videotoracoscopia  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish La imposibilidad técnica de realizar una vagotomía troncular transabdominal y la vagotomía incompleta constituyen un serio problema al tratar la patología ulcerosa, por lo que la vagotomía troncular transtorácica por videotorascoscopia se ha convertido en una opción preferencial para resolver tal co [...] ntingencia. Se reporta una serie de 7 pacientes a los que se les efectuó esta técnica, en el Servicio de Cirugía General del Hospital Clinicoquirúrgico "Hermanos Ameijeiras", de Ciudad de La Habana, en el período comprendido desde abril de 1996 hasta octubre de 1999. El 71,4 % de los pacientes tenía como proceder inicial vagotomía y piloroplastia y al 28,6 % se le había realizado solo un proceder de drenaje sin vagotomía troncular transabdominal, porque las condiciones locales o generales no lo permitieron. En el 57,1 % los síntomas de recidiva aparecieron antes del año de la operación inicial. El tiempo quirúrgico promedio fue de 80 min. Se reportó un accidente quirúrgico. La estadía posoperatoria promedio fue de 3 días. Del seguimiento resultó el 14,3 % de recidiva posoperatoria (n=1). No ocurrió mortalidad con el empleo de esta técnica Abstract in english The technical impossibility of performing a transabdominal truncal vagatomy and the incomplete vagotomy are a serious problem on treating ulcerous pathology, so the transthoracic truncal vagotomy by video-assisted thoracoscopy has become a preferential option to solve such contingency. A series of 7 [...] patients who underwent this technique at the General Surgery Service of "Hermanos Ameijeiras" Clinical and Surgical Hospital from April, 1996, to October, 1999, is reported. 71.4 % of the patients had undergone vagotomy and pyloroplasty as an initial procedure, whereas 28.6 % had undergone only a drainage procedure without traansabdomianl truncal vagotomy, since the local or general conditions did not allow to do something different. In 57.1 %, the relapse symptoms appeared before the year of the first operation. The average surgical time was 80 min. A surgical accident was reported. The average postoperative stay was 3 days. 14.3 % of the postoperative relapse (n = 1) was observed in the follow-up. No death occurred with the use of this technique

Mario M, Gómez Hernández; José M, Díaz Calderín; Pedro L, Vilorio Haza; Ricardo, Silvera García; Edelberto, Fuentes Valdés.

2002-08-01

293

Vagotomía transtorácica por videotoracoscopia  

Directory of Open Access Journals (Sweden)

Full Text Available La imposibilidad técnica de realizar una vagotomía troncular transabdominal y la vagotomía incompleta constituyen un serio problema al tratar la patología ulcerosa, por lo que la vagotomía troncular transtorácica por videotorascoscopia se ha convertido en una opción preferencial para resolver tal contingencia. Se reporta una serie de 7 pacientes a los que se les efectuó esta técnica, en el Servicio de Cirugía General del Hospital Clinicoquirúrgico "Hermanos Ameijeiras", de Ciudad de La Habana, en el período comprendido desde abril de 1996 hasta octubre de 1999. El 71,4 % de los pacientes tenía como proceder inicial vagotomía y piloroplastia y al 28,6 % se le había realizado solo un proceder de drenaje sin vagotomía troncular transabdominal, porque las condiciones locales o generales no lo permitieron. En el 57,1 % los síntomas de recidiva aparecieron antes del año de la operación inicial. El tiempo quirúrgico promedio fue de 80 min. Se reportó un accidente quirúrgico. La estadía posoperatoria promedio fue de 3 días. Del seguimiento resultó el 14,3 % de recidiva posoperatoria (n=1. No ocurrió mortalidad con el empleo de esta técnicaThe technical impossibility of performing a transabdominal truncal vagatomy and the incomplete vagotomy are a serious problem on treating ulcerous pathology, so the transthoracic truncal vagotomy by video-assisted thoracoscopy has become a preferential option to solve such contingency. A series of 7 patients who underwent this technique at the General Surgery Service of "Hermanos Ameijeiras" Clinical and Surgical Hospital from April, 1996, to October, 1999, is reported. 71.4 % of the patients had undergone vagotomy and pyloroplasty as an initial procedure, whereas 28.6 % had undergone only a drainage procedure without traansabdomianl truncal vagotomy, since the local or general conditions did not allow to do something different. In 57.1 %, the relapse symptoms appeared before the year of the first operation. The average surgical time was 80 min. A surgical accident was reported. The average postoperative stay was 3 days. 14.3 % of the postoperative relapse (n = 1 was observed in the follow-up. No death occurred with the use of this technique

Mario M Gómez Hernández

2002-08-01

294

Autoagresión por quemaduras  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Se realizó un estudio epidemiológico transversal en los casos de autoagresión por quemaduras, que acudieron al Cuerpo de Guardia del Servicio de Quemados del Hospital "Dr. Ernesto Guevara de la Serna", durante el período comprendido entre los meses de febrero de 1993 y febrero de 1994, con la finali [...] dad de determinar el comportamiento de algunas variables de interés en estos casos. El universo de estudio lo constituyeron 57 casos, cuya información se recolectó de la gráfica general del paciente quemado (Modelo 71-05) y de los registros estadísticos del Servicio de Quemados del Hospital. Como fundamentales resultados y conclusiones se obtienen las siguientes: la mayor frecuencia de casos se encontró en las edades comprendidas entre 15 y 24 años; el sexo femenino resultó ser el más afectado en todos los grupos de edades. La cocina fue el lugar donde se efectuó el acto con mayor frecuencia. Se encontró un alto grado de asociación entre la supervivencia y la presencia de antecedentes de intentos suicidas. Presentaron la mayor incidencia por municipios los de Puerto Padre y Jobabo; el alcohol y el kerosene fueron los agentes causales más utilizados. En la casuística predominaron los grandes quemados. Abstract in english A transversal epidemiological study of the cases of self-aggression by burns, who received medical attention at the Emergency Department of the Burns Service of the "Dr. Ernesto Guevara de la Serna" Hospital from February, 1993 to February, 1994, was carried out aimed at determining the behaviour of [...] some variables of interest in these cases. Fifty seven cases were studied and the information was collected from the general graph of the burn patient (Model 71-05), and from the statistical records of the Burns Service of the hospital. The main results and conclusions reached are the following: the highest frequency of cases was found in ages between 15 and 24; women were the most affected in all age groups; and kitchen was the place where the self-aggression was committed most of the times. A high degree of association between survival and the presence of previous suicide attempts was detected. Puerto Padre and Jobabo were the municipalities with greatest incidence; whereas alcohol and kerosene were the most used agents. Deep burns predominate in the casuistics.

Iris, Montes de Oca; José Manuel, Martínez Camejo; Páusides, Calixté Semanat; Angel L, Selva Suárez.

1996-04-01

295

Sepsis por shigella flexneri  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Se presenta un caso raro de sepsis por Shigella flexneri en una paciente de 45 años de edad quien estando hospitalizada para el estudio de un tumor cerebral, requirió el uso de manitol y dosis altas de corticoides; luego de ello presenta deposiciones líquidas con moco y sangre, desarrolla síndrome d [...] e respuesta inflamatoria sistémica, luego se aísla Shigella flexneri en el hemocultivo; recibió tratamiento antibiótico con ciprofloxacina. Se describen las características del caso y se comenta de acuerdo con la revisión de literatura. Abstract in english An unusual case of sepsis caused by Shigella flexneri in a 45-year old female patient is presented. While being hospitalized for diagnostic management of a brain tumor, she required mannitol and high-dose steroids; she had liquid and dysenteric stools, and soon afterwards she developed a systemic in [...] flammatory response syndrome. Shigella flexneri was isolated in the blood culture, and she was treated with ciprofloxacin. The case features are described and comments are presented, together with a review of the literature.

César, Cabrera C; Rosa, Purizaca F; Ana, León P; Juan, Celis S.

2005-04-01

296

Meningoencefalitis letal por Salmonella B  

OpenAIRE

La meningoencefalitis por bacilos gramnegativos ha ido incrementándose desde la década de los 70, con una mayor incidencia en niños pequeños, aunque existe una tendencia a aumentar en pacientes de la 3ra. edad. Dentro de este grupo de microorganismos, la causada por Salmonella sp, por su poca frecuencia, resulta una rareza. En este caso se presenta a una paciente de 80 años de edad con cuadro clínico de meningoencefalitis, que en el estudio del líquido cefalorraquídeo se aisló Salmon...

Herrera Valde?s, Nilda E.; Ma. Elena Fuerte Calvo; Ma. Elena Díaz García; Daysi Rodríguez Larrinaga; Martha Sandoval Acosta; Mario Santiago Puga Torres

2001-01-01

297

Conjuntivite granulomatosa atípica causada pela doença da arranhadura do gato: relato de caso  

Directory of Open Access Journals (Sweden)

Full Text Available Relatamos caso de paciente do sexo feminino, brasileira, 23 anos, residente na Alemanha, que cursou com quadro de conjuntivite granulomatosa bilateral crônica, sem acometimento ganglionar, não responsiva a tratamento tópico. A pesquisa laboratorial confirmou diagnóstico de conjuntivite por Bartonella henselae. O caso demonstra que a ausência de acometimento ganglionar não exclui o diagnóstico de doença da arranhadura do gato.

Oliveira Alexandre Hassler Príncipe de

2004-01-01

298

Por que sou rondoniano  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Numa revisão contextualizada no tempo e projetada pelos seus resultados, a obra do sertanista marechal Cândido Rondon (1867-1958) é avaliada positivamente pela dedicação à causa dos povos indígenas do Brasil, pela formação de uma ética de respeito do brasileiro para com os índios e pela instituciona [...] lização do dever do Estado brasileiro de proteger, assistir e ajudar os povos indígenas a manterem-se coesos e autônomos e ao mesmo tempo se projetarem no mundo mais amplo. A vida de Rondon é acompanhada desde a sua formação como cadete da Escola Militar, como membro da Igreja do Apostolado Positivista, como comandante da Expedição Rondon (1907-1930), que levou o telégrafo de Cuiabá a Porto Velho, e especialmente como criador e dirigente-mor do Serviço de Proteção aos Índios (SPI, 1910-1967). Rondon é o criador da expressão "Morrer se preciso for, matar nunca", que pautou a ação de indigenistas brasileiros que fizeram os primeiros contatos com muitos povos indígenas desde 1910. E muitos morreram seguindo essa norma, numa clara demonstração do novo espírito humanista criado pela atitude rondoniana. O indigenismo brasileiro implantado por Rondon teve altos e baixos ao longo de quase cem anos, que hoje prossegue pela Fundação Nacional do Índio (Funai). De fato, muitos povos indígenas perderam suas terras e muitos foram dizimados no século XX, mas os mais de 220 que sobreviveram vêm crescendo em número, obtendo suas terras (13% do território nacional) e conquistando novos espaços político-culturais no Brasil. Abstract in english This paper reviews the life and work of Brazilian Indianist Marshall Cândido Rondon. Rondon's life is marked by a profound dedication to the Brazilian Indian cause and to instilling in Brazilians a respect for Indian peoples. He influenced the Brazilian government in shaping a policy of protecting, [...] assisting, and helping Indian peoples retain cohesive, self-determined societies. He also encouraged Indians to project their destinies onto a larger political context. The paper follows Rondon during his days as a cadet in the Military School; as a devout member of the Church of the Positivist Apostolate; as commander of the Rondon Expedition (1907-1930), which stretched the telegraph from Cuiabá to Porto Velho; and especially as the founder and leader of the Indian Protection Service (1910-1967). The Indian Protection Service is the precursor to today's National Indian Foundation (Funai), founded in 1967). Rondon is the creator of the expression "Die if you must, never kill" which, since 1910, has served as the motto for many of the Brazilian indianists who made the first contacts with Indian peoples. Many of them died in service for the Indian cause, demonstrating the high purpose of this new kind of humanistic vision. The Indian policy established by Rondon has experienced ups and downs throughout the last 100 years. Indeed, several Indian tribes became extinct and many lost their lands in the 20th Century. However, of the more than 220 tribes that survive today the vast majority are growing in numbers, have had most of their lands demarcated (amounting to 13% of the Brazilian territory) and are conquering space in the Brazilian cultural-political panorama.

Mércio Pereira, Gomes.

299

Por que sou rondoniano  

Directory of Open Access Journals (Sweden)

Full Text Available Numa revisão contextualizada no tempo e projetada pelos seus resultados, a obra do sertanista marechal Cândido Rondon (1867-1958 é avaliada positivamente pela dedicação à causa dos povos indígenas do Brasil, pela formação de uma ética de respeito do brasileiro para com os índios e pela institucionalização do dever do Estado brasileiro de proteger, assistir e ajudar os povos indígenas a manterem-se coesos e autônomos e ao mesmo tempo se projetarem no mundo mais amplo. A vida de Rondon é acompanhada desde a sua formação como cadete da Escola Militar, como membro da Igreja do Apostolado Positivista, como comandante da Expedição Rondon (1907-1930, que levou o telégrafo de Cuiabá a Porto Velho, e especialmente como criador e dirigente-mor do Serviço de Proteção aos Índios (SPI, 1910-1967. Rondon é o criador da expressão "Morrer se preciso for, matar nunca", que pautou a ação de indigenistas brasileiros que fizeram os primeiros contatos com muitos povos indígenas desde 1910. E muitos morreram seguindo essa norma, numa clara demonstração do novo espírito humanista criado pela atitude rondoniana. O indigenismo brasileiro implantado por Rondon teve altos e baixos ao longo de quase cem anos, que hoje prossegue pela Fundação Nacional do Índio (Funai. De fato, muitos povos indígenas perderam suas terras e muitos foram dizimados no século XX, mas os mais de 220 que sobreviveram vêm crescendo em número, obtendo suas terras (13% do território nacional e conquistando novos espaços político-culturais no Brasil.This paper reviews the life and work of Brazilian Indianist Marshall Cândido Rondon. Rondon's life is marked by a profound dedication to the Brazilian Indian cause and to instilling in Brazilians a respect for Indian peoples. He influenced the Brazilian government in shaping a policy of protecting, assisting, and helping Indian peoples retain cohesive, self-determined societies. He also encouraged Indians to project their destinies onto a larger political context. The paper follows Rondon during his days as a cadet in the Military School; as a devout member of the Church of the Positivist Apostolate; as commander of the Rondon Expedition (1907-1930, which stretched the telegraph from Cuiabá to Porto Velho; and especially as the founder and leader of the Indian Protection Service (1910-1967. The Indian Protection Service is the precursor to today's National Indian Foundation (Funai, founded in 1967. Rondon is the creator of the expression "Die if you must, never kill" which, since 1910, has served as the motto for many of the Brazilian indianists who made the first contacts with Indian peoples. Many of them died in service for the Indian cause, demonstrating the high purpose of this new kind of humanistic vision. The Indian policy established by Rondon has experienced ups and downs throughout the last 100 years. Indeed, several Indian tribes became extinct and many lost their lands in the 20th Century. However, of the more than 220 tribes that survive today the vast majority are growing in numbers, have had most of their lands demarcated (amounting to 13% of the Brazilian territory and are conquering space in the Brazilian cultural-political panorama.

Mércio Pereira Gomes

2009-01-01

300

Estudo de parâmetros de soldadura por resistência, variante por pontos  

OpenAIRE

O presente trabalho propõe-se a estudar o grau de influência de cada parâmetro de soldadura por resistência por pontos no produto final. Para tal foram estudados os parâmetros de soldadura Intensidade da corrente I, Pressão p e Tempo de soldadura t, refletidos no valor da resposta selecionada, o diâmetro do ponto de soldadura. O estudo foi realizado com base num Planeamento de experiências, tendo sido utilizadas as técnicas de Análise de variância e o Algoritmo de Yates para a a...

Peixoto, Alzira Socorro Matos

2011-01-01

301

Educación mediada por las TICs  

OpenAIRE

El diseño de metodologías y el desarrollo de herramientas que permitan incorporar la Informática en el proceso de enseñanza y de aprendizaje constituyen temas de investigación y aplicación permanente dentro de las Ciencias de la Educación y de la Computación. La importancia de estos temas para ambas disciplinas está impulsada por el crecimiento de la (GXFDFLyQ D 'LVWDQFLD (hoy casi un sinónimo de educación mediada por las TICs), como una respuesta a los requerimientos crecientes ...

Depetris, Beatriz O.; Feierherd, Guillermo Eugenio; Gel, Mati?as; Carlomagno, Laura

2005-01-01

302

Visión por computador para UAS  

OpenAIRE

La visión por computador es una parte de la inteligencia artificial que tiene una aplicación industrial muy amplia, desde la detección de piezas defectuosas al control de movimientos de los robots para la fabricación de piezas. En el ámbito aeronáutico, la visión por computador es una herramienta de ayuda a la navegación, pudiendo usarse como complemento al sistema de navegación inercial, como complemento a un sistema de posicionamiento como el GPS, o como sistema de navegación visu...

Ponce Gonza?lez, Mo?nico

2012-01-01

303

Internamentos por Linfadenite Cervical num Serviço de Pediatria Geral / Admissions for cervical lymphadenitis in a General Paediatrics Unit  

Scientific Electronic Library Online (English)

Full Text Available SciELO Portugal | Language: Portuguese Abstract in portuguese Introdução: A Linfadenite Cervical (LAC) é uma entidade comum na idade pediátrica. As formas agudas bilaterais são as mais frequentes, de etiologia viral e autolimitadas. As agudas unilaterais são habitualmente bacterianas, provocadas pelo Streptococcus pyogenes e Staphylococcus aureus. Nas subaguda [...] s/crónicas a Bartonella, as Mycobacteria e o Toxoplasma devem ser etiologias consideradas. Objetivos: Caracterização de população de crianças internadas numa enfermagem de Pediatria Geral por LAC. Material e métodos: Estudo comparativo retrospetivo de uma amostra de conveniência que inclui as crianças internadas, entre Março de 1999 e Fevereiro de 2010. Resultados e Discussão: Identificaram-se 61 crianças, 88,5% do sexo feminino. A LAC aguda correspondeu a 88,5% dos casos, das quais 57,4% foram unilaterais. A forma subaguda/crónica ocorreu em 11,5%. A idade nas formas agudas foi significativamente inferior à das subagudas/crónicas (p=0,034). A etiologia bacteriana equivaleu a 96,7% sendo as restantes, uma mononucleose infeciosa e uma toxoplasmose ganglionar. O S. aureus e S. pyogenes corresponderam a 66,6% dos agentes bacterianos isolados. Identificaram-se três casos de LAC por Mycobacterium tuberculosis, dois por Bartonella henselae e dois por Ricketsia conorii. As infeções prévias da cabeça e pescoço estiveram presentes em 27 (44,3%). Documentaram-se infeções virais predisponentes em cinco casos. As localizações cervical e submandibular foram as mais frequentes, 47,5% e 44,3%, respetivamente. Evidenciou-se febre em 85,2% das crianças, sintomas regionais (torcicolo e trismos) em 45% e flutuação em 29%. Houve necessidade de drenagem cirúrgica em 24,6% dos doentes, em seis (9,8%) realizou-se citologia aspirativa e em 45 exames de imagem. A leucocitose (>15000/?L) e a proteína C reativa positiva (>3,0 mg/dL) verificaram-se em 83,6% e 65,5% dos casos, respetivamente. O S. pyogenes e o S. aureus cursaram com mais sinais inflamatórios e maior necessidade de drenagem cirúrgica (p=0,01). Os antibióticos foram utilizados em todos os doentes sendo o mais frequente a amoxicilina/ácido clavulânico (57,3%). A flucloxacilina administrou-se em 19,7% dos casos. A evolução foi favorável em todos os casos. Conclusão: O diagnóstico etiológico da LAC não é fácil na maioria dos casos. Embora a grande maioria das crianças com LAC não seja internada, existem alguns casos que, pela necessidade de investigação complementar ou de tratamento o sejam. Portugal é um país de média incidência de Tuberculose pelo que esta doença deve ser considerada, com especial atenção para a emergência de M. tuberculosis multirresistentes. Abstract in english Introduction: Cervical lymphadenitis (CL) is a common condition in children. Acute bilateral CL is the most frequent presentation, usually self-limited and caused by virus. Acute unilateral CL is commonly bacterial, most frequently caused by Streptococcus pyogenes and Staphylococcus aureus. Bartonel [...] la, Mycobacteria and Toxoplasma must be considered when the CL is subacute/chronic. Objective: Characterization of children with CL hospitalized in a paediatric unit. Population and methods: Retrospective comparative study of a convenience sample that includes inpatient children, between March 1999 and February 2010. Results and Discussion: Sixty-one patients were identified, 88,5% female. All CL were infectious. Acute CL was observed in 88,5% of cases (57,4% unilateral and 31,1% bilateral). Subacute/chronic CL occurred in 11,5%. The average age in acute cases was significantly lower than in subacute /chronic ones (p=0,034). Bacterial CL occurred in 96,7% and the remaining cases included infectious mononucleosis (n=1) and ganglionar toxoplasmosis (n=1). S. aureus and S. pyogenes were isolated in 66,6% of the patients. In addition, Mycobacterium tuberculosis was identified in three cases, Bartonella henselae in two and Ricketsia conorii in two. Previous head and neck

David, Lito; Diana, Pignatelli; Ana Sofia, Simões; Alexandra, Carvalho; Florbela, Cunha.

2013-12-01

304

Tallado de hélices por el método de copiado por proyección  

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Full Text Available Este dispositivo fue diseñado como parte de la investigación sobre Aerogeneradores que se está llevando a cabo en el Departamento de Ingeniería Mecánica, cuyo investigador principal es el Ing. Julio Mario Rodríguez Devis. Como parte inicial de la investigación se adecuó un túnel situado en el Laboratorio de Hidráulica para hacer las pruebas de modelos. En la construcción de dichos modelos se requiere una buena precisión en la hechura de las aspas del molino, en especial por el tamaño reducido de liste. Se debe garantizar que el perfil aerodinámico se conserve a lo largo del aspa, por lo que se requiere de un dispositivo capaz de tallar los álabes con la precisión y rapidez necesarias. Los resultados del trabajo fueron presentados al concurso del premio Worthington de la Ingeniería Colombiana 1982 (Categoría estudiantes, por los estudiantes que trabajaron en el proyecto, siendo acreedores al primer puesto.

José Vicente Fierro Vasco

2012-04-01

305

Meningoencefalitis letal por Salmonella B  

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Full Text Available La meningoencefalitis por bacilos gramnegativos ha ido incrementándose desde la década de los 70, con una mayor incidencia en niños pequeños, aunque existe una tendencia a aumentar en pacientes de la 3ra. edad. Dentro de este grupo de microorganismos, la causada por Salmonella sp, por su poca frecuencia, resulta una rareza. En este caso se presenta a una paciente de 80 años de edad con cuadro clínico de meningoencefalitis, que en el estudio del líquido cefalorraquídeo se aisló Salmonella grupo B serotipo typhimurium; la paciente fallece a los 5 días de su ingreso. La meningoencefalitis por Salmonella sp debe tenerse en cuenta en pacientes menores de 2 años de edad y ancianos, por la severidad del cuadro clínico y elevada mortalidad.Meningoencephalitis caused by gram-negative bacilli has increased since the 1970s, with a higher incidence in little children , although there is a a trend to rise in the elderly. Within this group of micororganisms, the meningoencephalitis caused by Salmonella sp is rare, since it is not very common. The case of an 80-year-old female patient with a clinical picture of meningoencephalitis is reported. Salmonella typhimurium serogroup B was isolated from the cerebrospinal fluid. The patient died 5 days after being admitted in the hospital. The meningoencephalitis caused by Salmonella sp should be taken into consideration in children under 2 and in the elederly because of the severity of the clinical picture and the elevated mortality.

Nilda E. Herrera Valdés

2001-06-01

306

Guía por las lindes transilvanas  

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Full Text Available Paul EluardRecorrer ahora el territorio dejado por Bram Stoker y su novela Drácula -a cien años de su primera edición- resulta sin duda caminar por las alucinantes fronteras del mundo delirante del vampiro como el hijo con- sentido de la nocturnidad y de las sombras. Aunque el terreno abonado es significativo, convocamos aquí un breve merodeo en relación con el tema en la literatura y su cuota de realidad; agregamos como postre un grupo de poemas y la conseja para prevenir la visita del vampiro.

Rafael Guillermo Serrano

1998-04-01

307

Alterações oftálmicas associadas à intoxicação experimental por closantel em caprinos Ophthalmic changes associated with the experimental poisoning by closantel in caprine  

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Full Text Available Alterações oftálmicas foram experimentalmente induzidas em caprinos após superdosagem com o anti-helmíntico closantel. Foram usados cinco caprinos com sete a oito meses de idade, produtos do cruzamento da raça Saanen com a Pardo Alpino. Os animais mostraram sinais de intoxicação entre quatro e cinco dias após a administração do closantel. Os sinais clínicos caracterizaram-se principalmente por distúrbios neurológicos centrais e cegueira. Ao exame clínico, observaram-se midríase bilateral, perda do reflexo pupilar à luz e cegueira bilateral. À oftalmoscopia indireta, foram observadas degeneração aguda de retina e papiledema. As alterações crônicas mostravam disco óptico acinzentado, atrofia de vasos e da retina. Nos fundos tapetal e não-tapetal notavam-se áreas de despigmentação e lesões irregulares castanho-amareladas. As alterações histológicas consistiam em perda dos neurônios da camada ganglionar e das células da camada nuclear interna e externa da retina. As alterações agudas no nervo óptico e na substância branca do encéfalo foram de degeneração espongiforme. As alterações crônicas do nervo óptico caracterizavam-se por extensa necrose e infiltração de células Gitter.Ophthalmic alterations were experimentally induced after overdose with the anthelmintic closantel. Five seven to eight- months-old, Saanen x Alpine caprine were used. The animals showed clinical signs of toxicosis four to five days after the administration of closantel. Clinical signs were primarily characterized by central nervous disturbances and blindness. Clinically, bilateral mydriasis, loss of pupillary light reflex, and blindness were observed. At indirect ophthalmoscopic examination, there was acute retinal degeneration and papilledema. Chronic ocular changes consisted of paleness of the optic disc, vascular atrophy, and retinal atrophy. Areas of pigment loss and irregular yellowish-brown foci were present in the tapetal and non-tapetal fundus. Histological alterations consisted of neuronal loss in the ganglion cell layer and depletion of cells in both the outer and inner nuclear layers of the retina. Acute changes of spongy degeneration were noted in the optic nerve and in the cerebral white matter. Chronic lesions in the optic nerve were characterized by extensive necrosis and infiltration by Gitter cells.

R. Ecco

2008-02-01

308

Alterações oftálmicas associadas à intoxicação experimental por closantel em caprinos / Ophthalmic changes associated with the experimental poisoning by closantel in caprine  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Alterações oftálmicas foram experimentalmente induzidas em caprinos após superdosagem com o anti-helmíntico closantel. Foram usados cinco caprinos com sete a oito meses de idade, produtos do cruzamento da raça Saanen com a Pardo Alpino. Os animais mostraram sinais de intoxicação entre quatro e cinco [...] dias após a administração do closantel. Os sinais clínicos caracterizaram-se principalmente por distúrbios neurológicos centrais e cegueira. Ao exame clínico, observaram-se midríase bilateral, perda do reflexo pupilar à luz e cegueira bilateral. À oftalmoscopia indireta, foram observadas degeneração aguda de retina e papiledema. As alterações crônicas mostravam disco óptico acinzentado, atrofia de vasos e da retina. Nos fundos tapetal e não-tapetal notavam-se áreas de despigmentação e lesões irregulares castanho-amareladas. As alterações histológicas consistiam em perda dos neurônios da camada ganglionar e das células da camada nuclear interna e externa da retina. As alterações agudas no nervo óptico e na substância branca do encéfalo foram de degeneração espongiforme. As alterações crônicas do nervo óptico caracterizavam-se por extensa necrose e infiltração de células Gitter. Abstract in english Ophthalmic alterations were experimentally induced after overdose with the anthelmintic closantel. Five seven to eight- months-old, Saanen x Alpine caprine were used. The animals showed clinical signs of toxicosis four to five days after the administration of closantel. Clinical signs were primarily [...] characterized by central nervous disturbances and blindness. Clinically, bilateral mydriasis, loss of pupillary light reflex, and blindness were observed. At indirect ophthalmoscopic examination, there was acute retinal degeneration and papilledema. Chronic ocular changes consisted of paleness of the optic disc, vascular atrophy, and retinal atrophy. Areas of pigment loss and irregular yellowish-brown foci were present in the tapetal and non-tapetal fundus. Histological alterations consisted of neuronal loss in the ganglion cell layer and depletion of cells in both the outer and inner nuclear layers of the retina. Acute changes of spongy degeneration were noted in the optic nerve and in the cerebral white matter. Chronic lesions in the optic nerve were characterized by extensive necrosis and infiltration by Gitter cells.

R., Ecco; C.S.L., Barros; D.L., Graça.

2008-02-01

309

Don Marcelino, visto por Unamuno  

OpenAIRE

[ES] El presente artículo analiza las relaciones entre Marcelino Menéndez y Pelayo y Miguel de Unamuno. Se centra fundamentalmente en las influencias ejercidas por Menéndez y Pelayo en Don Miguel a quien conoció en Madrid en sus años de estudiante Universitario.

Robles, Laureano

2008-01-01

310

Intoxicación por metales / Metal poisoning  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Los metales están entre los tóxicos más antiguos conocidos por el hombre. En el industrializado mundo actual las fuentes de exposición a metales son ubicuas tanto en el campo laboral como a partir de agua, los alimentos o el ambiente contaminados. Su toxicidad está caracterizada por el elemento metá [...] lico en cuestión pero se ve modificada por el tipo de compuesto, orgánico o inorgánico y sus características de hidro o liposolubilidad, que determina su toxicocinética y por tanto sus posibilidad de alcanzar sus dianas. Las biomoléculas más afectadas por los metales son las proteínas con actividad enzimática por lo que su patología es multisistema. Los principales sistemas afectados son el gastrointestinal, neurológico central y periférico, hemático y renal. Algunos de los compuestos metálicos son carcinógenos. Los metales se benefician de un tratamiento condicionado por su reactividad química. Pueden ser inactivados y eliminados mediante la administración de substancias quelantes que producen con ellos moléculas complejas, atóxicas y excretables. Los principales agentes quelantes son: BAL (British Anti-Lewisite o dimercaprol), DMPS (ácido 2,3-dimercapto-1-propanosulfonico) y DMSA (ácido meso-2,3-dimercatosuccínico o Succimer), EDTA, Penicilamina (ß,ß-dimetilcisteína) y Desferoxamina. Se exponen a continuación las características toxicocinéticas, mecanismo de acción, clínica y tratamiento de alguno de los metales y metaloides más relevantes: plomo, mercurio y arsénico. Abstract in english Metals are amongst the oldest toxic substances known to man. In today’s industrialized world the sources of exposure to metals are ubiquitous both in the field of work and from polluted water, foodstuffs and the environment. Their toxicity is characterized by the metallic element in question, but th [...] is is modified by the type of compound, whether organic or inorganic, and its characteristics of hydrosolubility and liposolubility, which determines its toxicokinetics and thus the possibilities of it reaching its targets. The biomolecules most affected by metals are the proteins with enzymatic activity, which is why their pathology is multisystemic. The principal systems affected are the gastrointestinal, central and peripheral neurological, haematic and renal. Some metallic compounds are carcinogenic. Metals’s treatment is conditioned by their chemical reactivity. They can be deactivated and eliminated by the administering of chelating agents that produce complex molecules, which are non-toxic and can be excreted. The principal chelating agents are: BAL (British Anti-Lewisite or dimercaprol) DMPS (2,3-Dimercapto-1-propanesulfonic Acid) and DMSA (meso-2,3-Dimercaptosuccinic or Succimer), EDTA, Penicilamine (ß,ß-dimethylcysteine) and Deferoxamine. Toxicokinetic characteristics, mechanism of action, clinical picture and treatment of some of the most relevant metals and metalloids: lead, mercury and arsenic, are considered.

A., Ferrer.

311

Colitis por desviación Diversión colitis  

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Full Text Available La colitis por desviación es la alteración inflamatoria de la mucosa colorrectal que se produce por la desviaciónde las heces (cd, la cual priva a los "colonocitos" de su principal substrato metabólico, como son los ácidos grasos de cadena corta, producidos por la fermentación bacteriana de almidones y proteínas. Se presenta el caso de una paciente con esta alteración. La mucosa del área desfuncionalizada tiene alteraciones muy variadas: eritema, hemorragias petequiales, fiabilidad con sangrado fácil, nodularidad (por hiperplasia de folículos linfoides y ocasionalmente ulceraciones aftoides. Los cambios histológicos dependen de la enfermedad subyacente previa a la desviación. Cuando la mucosa previa ES normal, la inflamación está confinada a la mucosa y se caracteriza por infiltrado linfoplasmocitario, mínima distorsión de la arquitectura de las criptas y la característica hiperplasia linfoide. Ocasionalmente puede haber abscesos de las criptas acompañados por depleción de moco. Los cambios inflamatorios crónicos aparecen dentro de los tres meses de la desviación. Esta entidad puede ser asintomática o presentar diarrea escasa con sangrado rectal o secreción de moco o dolor abdominal. Se debe diferenciar de la colitis crónica de cualquier etiología. En casos de enfermedad inflamatoria previa (colitis ulcerativa o enfermedad de Crohn, el principal diagnóstico diferencial, hay recurrencia de la enfermedad en la mucosa que ha sido privada de la materia fecal la cual le provee sus substratos metabólicos. El tratamiento frecuentemente ofrecido son los enemas con ácidos grasos de cadena corta (acético, propiónico y butírico, aunque los resultados son contradictorios.Fecal stream diversion is the cause of inflammatory bowel disease secondary to the absence of luminal short chain fatty acids that provide nutrients to the colonocyte. The endoscopic findings include edema, erythema, and friability. Ulceration and nodularity could appear. The pathology findings are variable, and depend on the pre-existing disease. The inflammatory changes become apparent three months after surgical diversion. The patient can appear asymptomatic, and progressively abdominal pain, mucoid and bloody discharge are the subsequent manifestations. Differential diagnosis includes any chronic colitis, ulcerative colitis, and Crohn’s disease, and the fist one is the previous disease. The treatment frequently offered is short chain fatty acids by rectal enemas, but trial results are contradictory.

María Claudia Quiroga G

312

Colitis por desviación / Diversión colitis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish La colitis por desviación es la alteración inflamatoria de la mucosa colorrectal que se produce por la desviaciónde las heces (cd), la cual priva a los "colonocitos" de su principal substrato metabólico, como son los ácidos grasos de cadena corta, producidos por la fermentación bacteriana de almidon [...] es y proteínas. Se presenta el caso de una paciente con esta alteración. La mucosa del área desfuncionalizada tiene alteraciones muy variadas: eritema, hemorragias petequiales, fiabilidad con sangrado fácil, nodularidad (por hiperplasia de folículos linfoides) y ocasionalmente ulceraciones aftoides. Los cambios histológicos dependen de la enfermedad subyacente previa a la desviación. Cuando la mucosa previa ES normal, la inflamación está confinada a la mucosa y se caracteriza por infiltrado linfoplasmocitario, mínima distorsión de la arquitectura de las criptas y la característica hiperplasia linfoide. Ocasionalmente puede haber abscesos de las criptas acompañados por depleción de moco. Los cambios inflamatorios crónicos aparecen dentro de los tres meses de la desviación. Esta entidad puede ser asintomática o presentar diarrea escasa con sangrado rectal o secreción de moco o dolor abdominal. Se debe diferenciar de la colitis crónica de cualquier etiología. En casos de enfermedad inflamatoria previa (colitis ulcerativa o enfermedad de Crohn), el principal diagnóstico diferencial, hay recurrencia de la enfermedad en la mucosa que ha sido privada de la materia fecal la cual le provee sus substratos metabólicos. El tratamiento frecuentemente ofrecido son los enemas con ácidos grasos de cadena corta (acético, propiónico y butírico), aunque los resultados son contradictorios. Abstract in english Fecal stream diversion is the cause of inflammatory bowel disease secondary to the absence of luminal short chain fatty acids that provide nutrients to the colonocyte. The endoscopic findings include edema, erythema, and friability. Ulceration and nodularity could appear. The pathology findings are [...] variable, and depend on the pre-existing disease. The inflammatory changes become apparent three months after surgical diversion. The patient can appear asymptomatic, and progressively abdominal pain, mucoid and bloody discharge are the subsequent manifestations. Differential diagnosis includes any chronic colitis, ulcerative colitis, and Crohn’s disease, and the fist one is the previous disease. The treatment frequently offered is short chain fatty acids by rectal enemas, but trial results are contradictory.

María Claudia, Quiroga G; William, Otero R; Jorge Eduardo, Caminos P; Martín, Gómez.

2007-06-30

313

toxicidad neuroendocrina inducida por cadmio  

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Full Text Available El cadmio es un agente químico tóxico importante debido a su creciente nivel en el medio ambiente como resultado de prácticas industriales y agrícolas. Como perturbador endocrino, el cadmio modifica la secreción de hormonas hipofisarias. Los efectos indirectos del cadmio provocan la generación de especies reactivas de oxígeno y reducen la actividad de las proteínas implicadas en las defensas antioxidantes. La melatonina es conocida como un potente antioxidante, scavenger de radicales libres y quelante de metales sintetizada en la glándula pineal. De esta manera, las acciones antioxidantes de esta indolamina protegen frente a la peroxidación lipídica y el daño oxidativo de los radicales y de sus productos tóxicos. Los trabajos recopilados en esta revisión ponen de manifiesto la capacidad antioxidante de la melatonina y, por lo tanto, su posible papel protector frente a la toxicidad del cadmio en lo que se refiere al estrés oxidativo y peroxidación lipídica inducidos por la exposición a este metal.

A Romero

2008-01-01

314

A rodar por los barrios  

OpenAIRE

A rodar por los Barrios es un proyecto de capacitación con jóvenes -que fue ejecutado en conjunto con el Municipio de Moreno- tendiente a propiciar la participación en estrategias locales de desarrollo. Este proceso nace de la idea de promover la producción de mensajes en el lenguaje audiovisual, a través de talleres participativos que pongan en juego la creatividad, las experiencias y los saberes propios. No se apunta solamente a trabajar las herramientas del lenguaje, sino tambi...

Cassan, Dari?o Alfredo; Fabi, Jacqueline; Polla, Mari?a Carla

2012-01-01

315

Suicidio por salto al vacío  

OpenAIRE

La tendencia al suicidio se asocia con problemas de soledad, abandono sicológico en la infancia, drogadicción, enfermedades graves, etc. Y en pacientes psiquiátricos es más común tras ser dados de alta. En Argentina los suicidios aumentaron en 25% de 1990 a 1992, y el 78 % de las víctimas fueron de sexo masculino. La proporción por salto al vacío aumentó , especialmente en áreas urbanas. En 40 casos argentinos hubo introducción basal del fémur (25%), fractura del calcáneo en 17%,...

Linares, Braulio Ernesto P.; Félix Antonio Bikic

1995-01-01

316

Intoxicação por Monóxido de Carbono  

OpenAIRE

O monóxido de carbono é uma das principais causas de intoxicação, podendo ser letal ou levar a sequelas neuropsicológicas tardias. A intoxicação manifesta-se por sintomas pouco especificos, pelo que o seu diagnóstico exige um elevado índice de suspeição. 0 doseamento de carboxihemoglobina e útil, mas apresenta uma fraca correlação quer com a clínica, quer com o prognóstico. O tratamento baseia-se na oxigenação adequada dos tecidos, não sendo ainda consensuais os crit...

Conde, A.; Alegria, A.; Neto, A.

2004-01-01

317

Quemadura por rayo / Lightning burn  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Fundamento: las quemaduras eléctricas producidas por fenómenos atmosféricos probablemente fueron las primeras y tiene una alta mortalidad. Objetivo: describir el cuadro clínico de un paciente que presentó una descarga eléctrica natural y sobrevivió al evento. Caso clínico: paciente de 37 años de eda [...] d, masculino, sin antecedentes patológicos, fue alcanzado por un rayo que conllevó a la pérdida de conciencia por varios minutos, así como quemaduras en cara, zonas del tronco anterior y posterior y miembro superior derecho que se diagnosticaron como lesiones dérmicas A de un 22 % de superficie corporal quemada, con pérdida de conciencia momentánea y complicación renal y oftalmológica posteriormente, sobrevivió al evento inicial. Conclusiones: las quemaduras por electricidad natural o fulguraciones constituyen un evento dramático con graves complicaciones y alta mortalidad. Abstract in english Background: electric burns produced by atmospheric phenomena were probably the first type of burn and have a high mortality. Objective: to describe the clinical manifestations of a patient who got a natural discharge and survived. Clinical case: a thirty-seven-year-old male patient without pathologi [...] cal records was struck by a lightning that caused the loss of consciousness for some minutes, as well as burns of the face, the torso, the back, and the right fore limb that were diagnosed as A dermic lesions of a 22 % of the body burned. Subsequently, the patient presented momentary loss of consciousness, and ophthalmic and kidney complications. He survived the initial event. Conclusions: burns by natural electricity and fulgurations constitute a dramatic event with severe complications and a high mortality.

Enrique J., Moya Rosa; Yadira, Moya Corrales.

2013-12-01

318

Toxicidad por óxido de etileno  

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Full Text Available

El óxido de etileno, es un compuesto epóxido gaseoso, ampliamente utilizado en nuestro medio como agente esterilizante y de desinfección en instituciones hospitalarias. Los estudios realizados en varios países han demostrado efectos sobre la salud humana, caracterizados por  hipersensibilidad dérmica, irritación del tracto respiratorio, irritación ocular, excitación del S.N.C. y se asocia con presencia de abortos expontáneos, aberraciones cromosómicas e irritación crónica de laringe y tráquea en personal expuesto ocupacionalmente.

Téllez M. Jairo A.

1993-08-01

319

Intoxicación por drogas Drug poisoning  

OpenAIRE

El consumo de drogas ilegales en nuestro país ha experimentado un notable cambio en los últimos años, relegando a la heroína e incorporando la cocaína, los derivados anfetamínicos como el "éxtasis" (MDMA), el "éxtasis líquido" (GHB) y, en menor medida, la ketamina. Se lleva a cabo una revisión de la intoxicación aguda por opiáceos y de su tratamiento en los servicios de urgencias, teniendo en cuenta el descenso progresivo de los casos que se presentan con el advenimiento de nuevas...

Gainza, I.; Nogue?, S.; Marti?nez Velasco, C.; Hoffman, R. S.; Burillo-putze, G.; Duen?as, A.; Go?mez, J.; Pinillos, M. A.

2003-01-01

320

por mulheres assistidas em grupo  

Directory of Open Access Journals (Sweden)

Full Text Available El objetivo de este estudio, de carácter cualitativo, fue comprender el significado atribuido por la mujer a las experiencias vividas en relación a la sexualidad en el climaterio. Participaron ocho mujeres que frecuentaron grupos de climaterio en una Unidad de Salud de la Familia, en Juiz de Fora - MG. Los datos fueron recolectados por medio de entrevistas parcialmente elaboradas, y organizados a partir de la convergencia de los discursos sobre los cambios en la sexualidad. Los resultados mostraron que la mujer evidencia alteraciones en la vida sexual, caracterizadas por la sequedad vaginal y la disminución o ausencia de la libido sexual. Otros factores que interfieren directamente en su sexualidad son: baja autoestima, falta de diálogo e incomprensión del compañero afectivo. El grupo de climaterio fue para ellas un espacio que les posibilitó el intercambio de experiencias, permitiéndoles una mayor comprensión de ese momento. El estudio aporta reflexiones acerca de la vivencia de la sexualidad de la mujer en el climaterio, entendiendo que las interfaces que la componen están en la esfera biopsicosocial.

De\\u00EDse Moura de Oliveira

2008-01-01

321

Fisiología cerebral por imágenes: Difusión por tensión - tractografía  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish Objetivo. Analizar la contribución de la difusión por tensión-tractografía a la información acerca de la integridad y función de los grandes tractos de fibras de la sustancia blanca en sujetos normales y en algunos casos patológicos. Material y método. Se analizan los estudios de difusión por tensió [...] n tractografía obtenidos por un resonador de 1.5 T, PHILIPS Intera Achieva. Con secuencias de tractografía, scan de 7 minutos, TR-6.707, TE-89, VOXEL en mm 1.44 x 1.44 x 300, GAP 0.3, FOV 230 mm, Slice 3, adquisiciones 4, Matriz 112 x 116, con técnica de EPI, Single Shot, utilizando 16 direcciones en diez voluntarios normales y en tres pacientes uno con un angioma, otro con un oligodendroastrocitoma y otro con una lesión axonal difusa. Resultados. En todos los casos normales se pudieron visualizar los tractos de la sustancia blanca hemisférica. En los pacientes, se observó el desplazamiento de las fibras en uno, el desplazamiento y la no infiltración en otro y en el tercero, la destrucción parcial de los tractos. Conclusión. La difusión por tensión-tractografía es de gran utilidad para valorar los fascículos o haces elocuentes, e incluso estudiar su estado funcional (Fracción Anisotrópica). Abstract in english Objective: to analyze the contribution of diffusion MR tractography imaging to determine the value of the integrity and function of the great tracts of fibers in the white matter in healthy volunteers and patients. Material and method: diffusion MR tractography imaging was analyzed using with a 1.5- [...] T , PHILIPS Intera Archieva, 7 min. of scans, TR-6.707, TE-89, 1.44 x 1.44 x 300 mm VOXEL, 0.3 GAP, 230 mm FOV, 3 mm thick slices ,acquisition 4, 112 x 116 MATRIX, EPI tec., Single Shot 16 directions in 10 healthy volunteers and 3 patients, one of them with angioma malformation, another one with oligodendroglioma, and the last case with diffuse axonal injury. Results: the tracts in the white matter were observed in all healthy volunteers. Regarding to patients, we observed displacement of fiber in one of them, displacement and non-infiltration in another one, and partial damage of the tracts in the last one. Conclusion: diffusion MR tractography imaging is of great value for observed the fiber and beam elocuent, including their functional state.

Manuel, Martínez; Alberto, Prosen; Carlo, Castillo; Carlos J, Morales; Claudio, Bruno.

2007-06-01

322

INTOXICACIÓN POR ORGANOFOSFORADOS / ORGANOPHOSPHORUS POISONING / INTOXICAÇÃO POR ORGANOFOSFORADO  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in portuguese Os compostos organofosforados sãoum grupo de substânciasorgânicas derivadas da estrutura química de fósforo e temum grande número de aplicações e usos. Têm sido utilizados como aditivos para óleos, solventes, indústrias de tintas, vernizes, couro artificial, isolaçãoelétrica, impermeabilização, amac [...] iadores de plástico, fungicidas, inseticidas, entre outros. Dada a suaampladistribuição e uso em diferentes indústrias e na agricultura, é muitocomum ter intoxicaçõesacidentais por essescompostos, alémdisso, substâncias que estãodisponíveis para os indivíduos, têm sido usadas como substância tóxica emsuicídios. O quadro de intoxicaçãoproduzuma síndrome clínica característica, comsintomas colinérgica secundários à estimulação dos receptores de acetilcolina. A conduta médica destes pacientes pode significar a diferença entre a vida eamorte, por isso é importante que todo o pessoal de saúde nos serviços de emergênciasejatreinado para reconhecer a figura de intoxicação e rapidamente tratar adequadamente. Abstract in spanish Los compuestos organofosforados son un grupo de sustancias orgánicas derivadas de la estructura química del fósforo y tienen un gran número de aplicaciones y utilidades. Han sido utilizados como aditivos del petróleo, disolventes, en las industrias de colorantes, barnices, cuero artificial, aislante [...] s eléctricos, impermeabilizantes, ablandadores de plásticos, fungicidas, insecticidas entre otros. Dado su amplia distribución y uso en diferentes industrias y en la agricultura, es muy frecuente que se presenten intoxicaciones accidentales por estos compuestos; además, como son sustancias que están al alcance de las personas, han sido empleadas como tóxicos en suicidios. El cuadro de intoxicación genera un síndrome clínico característico, con síntomas colinérgicos secundarios a la estimulación de los receptores de acetilcolina. El manejo médico de estos pacientes puede significar la diferencia entre la vida y la muerte, por lo que es importante que todo el personal de salud en los servicios de urgencias esté capacitado para reconocer el cuadro de intoxicación y manejarlo rápidamente en forma adecuada. Abstract in english Organophosphorus compounds are a group of organic substances derived from the chemical structure of phosphorus. They have a large number of applications and utilities. They are used as oil additives, solvents, in the industry of dyes, varnishes, artificial leather, electrical insulation, waterproofi [...] ng, plastic softeners, fungicides and others. Given its wide distribution and use in different industries and trades, organophosphorus compounds can be taken accidentally causing intoxication and can be used with the intention to commit suicide. The intoxication with organophosphorus compounds produces a characteristic clinical syndrome with cholinergic symptoms after stimulation of acetylcholine receptors. The medical management of these patients could make the difference between life and death, hence the physician and all health personnel in the emergency department should be able to recognize and manage poisoning symptomatology properly.

DANIEL G, FERNÁNDEZ A.; LILIANA C., MANCIPE G.; DIANA C., FERNÁNDEZ A..

2010-06-01

323

Responsabilidade civil por atos legislativos do estado  

OpenAIRE

[ES] Esta tesis trata del estudio y análisis crítico de un fenómeno jurídico que sigue a la espera de una mejor apreciación, tanto por la doctrina (campo dogmático) como por la jurisprudencia (campo pragmático): la responsabilidad civil generada por los actos legislativos del Estado.

Melo, Hila?rio Castro

2010-01-01

324

Intoxicación por amitraz / Amitraz poisoning  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish En nuestro medio, la intoxicación por amitraz y su solvente xileno es una patología poco frecuente, pero puede observarse un aumento en zonas rurales, donde se lo emplea como insecticida-ectoparasiticida.1-3 Por tal razón, se cuenta con escasa bibliografía que oriente su manejo en niños. Presentamos [...] el caso de un paciente de 2 años de edad, con ingestión accidental de amitraz, internado en nuestra Unidad de Cuidados Intensivos Pediátricos y que requirió ventilación mecánica. Creemos que comunicar este caso puede ser útil para alertar sobre esta intoxicación, poco frecuente en nuestro medio. Abstract in english Poisoning due to amitraz together with its solvent xilene, is an unusual condition although may be increasing in rural areas where it is used as insecticide-ectoparasiticide.1-3 At present, there is scare references to orient physicians concerning its handling in childhood. We present the case of a [...] 2-year-old boy who suffered an accidental intake of amitraz and was admitted into our Pediatric Intensive Care Unit requiring mechanical ventilation. We consider the usefulness of informing the medical community about this case so as to be aware of this rare kind of poisoning in our community.

C. Gustavo, Caprotta; Marcelo, Martínez; Martín, Tiszler; Verónica, Guerra.

2009-10-01

325

Contaminación acústica por grupos electrógenos  

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Full Text Available El trabajo tiene como tema el impacto ambiental por ruido de grupos electrógenos (GE. Se muestran diferentes definiciones y conceptos referidos a la explotación de los GE y las consecuencias que puede provocar a las personas que permanezcan cerca de ellos durante su funcionamiento. Los grupos electrógenos son además de generadores de energía, generadores de ruido y por tanto contribuyen a la contaminación acústica del lugar donde estén situados. En el documento se aborda la importancia del correcto uso y selección de los protectores auditivos para aquellas personas que trabajen directamente en las baterías de grupos electrógenos, así como algunas formas de atenuar el ruido. También se caracteriza la emisión sonora de estos a partir de estudios realizados.This paper concerns with the environmental damage due to noise emission introduced by Engine Generator Sets. Definitions and concepts about Engine Generator operation are shown as well as the consequences over the personnel who stand near the Engine Generators during their operation. Obviously, such kind of machines generates energy but they also generate noise which contaminates the environment around them. The paper states how important are the right selection and use of noise protection devices for those whose main job is to operate engine generator sets. Some methods for noise damping are also shown. Finally, some case studies are presented in order to describe the noise emission registered in the real world.

Yanexy Cepero-Aguilera

2009-05-01

326

Trastornos Psiquiátricos Inducidos por Médicamentos  

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Full Text Available Los médicamentos psicotrópicos pueden producir efectos farmacológicos adversos,y que las médicaciones usadas en Medicina para tratar las enfermedades no psiquiátricas pueden causar complicaciones conductuales. El especialista en Psiquiatría debe estar familiarizado con una amplia variedad de reacciones adversas ante los médicamentos. Además, el médico de otras especialidades debe estar alerta con respecto a las interacciones potenciales entre las médicaciones que él prescribe y las recetadas simultáneamente por los colegas de otras especialidades.La presencia de enfermedad psiquiátrica, disfunción cerebral orgánica, o función hepática o renal alterada pueden predisponer a un paciente en particular a estos efectos secundarios.Los variados médicamentos no utilizados de forma psiquiátrica y neurológica pueden provocar un sin número de alteraciones sobre el SNC y su correspondiente efecto sobre la actividad mental del paciente, tratado por diversas patologías somáticas. Si bien sonbastante conocidos los efectos más comunes como el Síndrome Confusional Agudo, Alteraciones del Ánimo o cuadros Psicóticos, sus efectos son mucho más amplios de los observados comúnmente.

Jonny Román Durandal Montaño

2011-06-01

327

AMBIENTE POR LOS PUEBLOS INDÍGENAS  

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Full Text Available En la actualidad existe una tendencia internacional hacia el reconocimiento de los derechos colectivos de los pueblos indígenas, especialmente los referidos a la posesión de la tierra y a la protección del medio ambiente. Algunos países latinoamericanos han hecho un importan­ te avance en este sentido, al reconocer constitucionalmente la posesión y personalidad jurídica de los pueblos indígenas en sus territorios, lo que no ha sido posible todavía en México, en donde existe una falta de reconocimiento sobre la titularidad de sus tierras, así como al uso y acceso a los recursos naturales que ahí se encuentran, lo que genera inseguridad jurídica e influye nega- tivamente en las posibles acciones de protección, conservación y defensa legal del medio ambien- te por parte de los pueblos indígenas. En el presente texto se analizan el derecho al respeto, a la integridad y conservación de su hábitat natural así como al derecho a los recursos naturales, siendo este último elemento la base del análisis, al entender que la protección medioambiental por parte de los pueblos indígenas está íntimamente ligada a la defensa de su tierra, así como al reconocimiento de su derecho a la misma y a la conservación de los recursos que en ella se en- cuentren.

Georgina Gaona Pando

2013-01-01

328

Coriorretinitis por toxoplasma en niños  

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Full Text Available En un período de 5 años fueron atendidos en el Instituto de Medicina Tropical "Pedro Kourí", 21 niños con lesiones oculares, debidas al toxoplasma (coriorretinitis por toxoplasma. De los 21 niños, 5 presentaron lesiones en ambos ojos (23,8 %, y 16 (76,1 % en un solo ojo (9 en el ojo izquierdo y 7 en el ojo derecho. El 42,8 % (9 llegó con lesiones activas con resultados satisfactorios al tratamiento médico, y el 57,1 % (12 con lesiones cicatrizadas, que en este estadio no necesitan tratamiento. Todos los niños fueron seguidos por consulta externa con una evolución favorable.In a period of 5 years, 21 children with ocular lesions caused by toxoplasma (chorioretinitis due to toxoplasma were seen at "Pedro Kourí" Tropical Medicine Institute. Of the 21 children, 5 (23,8 % had lesions in both eyes and 16 (76,1 % in one eye (9 in the left eye and 7 in the right one. 9 children (42,8 % initially had active lesions that responded satisfactorily to medical treatment whereas 12 (57,1 % came to the hospital with healed lesions that did not require any treatment. They were all followed up as outpatients with favorable evolution in all cases.

Ida González Núñez

1999-08-01

329

Coriorretinitis por toxoplasma en niños  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish En un período de 5 años fueron atendidos en el Instituto de Medicina Tropical "Pedro Kourí", 21 niños con lesiones oculares, debidas al toxoplasma (coriorretinitis por toxoplasma). De los 21 niños, 5 presentaron lesiones en ambos ojos (23,8 %), y 16 (76,1 %) en un solo ojo (9 en el ojo izquierdo y 7 [...] en el ojo derecho). El 42,8 % (9) llegó con lesiones activas con resultados satisfactorios al tratamiento médico, y el 57,1 % (12) con lesiones cicatrizadas, que en este estadio no necesitan tratamiento. Todos los niños fueron seguidos por consulta externa con una evolución favorable. Abstract in english In a period of 5 years, 21 children with ocular lesions caused by toxoplasma (chorioretinitis due to toxoplasma) were seen at "Pedro Kourí" Tropical Medicine Institute. Of the 21 children, 5 (23,8 %) had lesions in both eyes and 16 (76,1 %) in one eye (9 in the left eye and 7 in the right one). 9 ch [...] ildren (42,8 %) initially had active lesions that responded satisfactorily to medical treatment whereas 12 (57,1 %) came to the hospital with healed lesions that did not require any treatment. They were all followed up as outpatients with favorable evolution in all cases.

Ida, González Núñez; Manuel, Díaz Jidy; Jorge, Pérez Ávila.

1999-08-01

330

REANASTOMOSIS TUBÁRICA POR VÍA LAPAROSCÓPICA  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Antecedentes: La esterilización tubárica se realiza en un número importante de pacientes jóvenes que por diferentes motivos desean posteriormente un nuevo embarazo, porcentaje que varía entre el 1,3-15%. Hasta ahora la cirugía ha sido la primera alternativa de tratamiento y gracias a la evolución de [...] la técnica y el desarrollo de material quirúrgico, es posible de realizar por vía laparoscópica. Objetivos: Hacer una síntesis de la historia y técnicas utilizadas en la reanastomosis tubárica, los criterios de selección, el estudio preoperatorio, los factores pronósticos y comparar los resultados de la laparotomía y la laparoscopia. Métodos: Se realiza búsqueda en base de datos Medline PubMed usando las palabras clave: tubal anastomosis, reversal of esterilization, infertility, microsurgery, tubal esterilization; se seleccionan todas aquellas publicaciones tipo review de reanastomosis tubárica con microcirugía abierta y todas aquellas de reanastomosis por vía laparoscópica. Resultados: La reanastomosis tubárica por laparotomía con técnica microquirúrgica tiene un éxito entre 60 y 90% de tasas de embarazo. Las primeras publicaciones de la técnica laparoscópica no presentan resultados satisfactorios, sin embargo casuísticas más numerosas y especialmente las que muestran la técnica microlaparoscópica tienen resultados similares con todas las ventajas de la laparoscopia. Conclusión: La recanalización tubaria por vía laparoscopica es una técnica factible, probada y exitosa, alternativa a la laparotomía, sin embargo, para su implementación con resultados satisfactorios es necesario contar con equipamiento apropiado, experiencia previa en cirugía abierta y entrenamiento en cirugía endoscópica Abstract in english Background: Tubal ligation is used in a large number of young patients, who frequently want a new pregnancy, the percentage rate ranges from 1.3 to 15%. Until now the standard treatment has been the microsurgical tubal anastomosis through laparotomy, however, the development of new techniques and in [...] struments allowed a laparoscopic approach for this surgery. Objectives: to make a synthesis of the hystory and operatory technics in laparoscopic tubal anastomosis to evaluate the inclusions criteria, the preoperative work-up, the factors of prognosis and to make a comparison between laparotomy and laparoscopy. Methods: We make a search in medline database (PubMed), with the key words: tubal anastomosis, reversal of sterilization, infertility, microsurgery, tubal sterilization, and selecting all of the review publications in microsurgical tubal reanastomosis by laparotomy and all of publications of the laparoscopic technic. Results: The microsurgical tubal anastomosis through laparotomy has a pregnancy rate ranging from 70% to 80%. The first publications whith the laparoscopic technic show dissapointed results, however, papers with bigger casuistics and with the microlaparoscopic technic show the same results that the open technic with all of the advantages of the laparoscopy. Conclusion: Laparoscopic tubal reversal is a feasible, tested and successful alternative to the traditional surgery performed by laparotomy, however, to achieve a successful outcome it is necessary a meticulous surgical technique, adequate equipment and extensive experience with tubal anastomosis by laparotomy and advances laparoscopic techniques

Juan Raúl, Escalona M; Benoît, Rabischong; Erdogan, Nohuz; Ana María, Moraga P.

331

Intoxicación por plaguicidas / Pesticide poisoning  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Los plaguicidas son una de las familias de productos químicos más ampliamente empleadas por el hombre. Se han usado sobre todo para combatir plagas por su acción sobre las cosechas o como vectores de enfermedades transmisibles. Los plaguicidas pueden clasificarse en función de su empleo (insecticida [...] s, fungicidas, herbicidas, raticidas…) o de su familia química (organoclorados, organofosforados, carbamatos, piretoides, compuestos bipiridílicos, sales inorgánicas…). Todos ellos son biocidas lo que implica, habitualmente una alta toxicidad humana que ha sido motivo de preocupación desde mitad del siglo XX debido al amplio e indiscriminado empleo de estos productos. La exposición a los plaguicidas puede tener efectos agudos, crónicos y a largo plazo. Algunos compuestos organoclorados (como el DDT) fueron los primeros en ser empleado en fumigaciones masivas para combatir la malaria y han debido ser prohibidos debido a su capacidad de bioacumulación y persistencia medioambiental. El peligro representado por la generalizada presencia de estos agentes, se ha demostrado en los numerosos episodios de epidemias tóxicas humanas, productoras de alta morbi-mortalidad, descritas por casi todas las familias químicas: insecticidas y fungicidas organoclorados, insecticidas organofosforados y carbamatos, fungicidas organomercuriales y sales inorgánicas. Estos episodios se han producido sobre todo por vía alimentaria y en el terreno profesional. Otras causas de preocupación sanitaria son su capacidad carcinogénica y de ocasionar alteraciones reproductivas. Se presentan las principales características de algunas de las familias más relevantes. Abstract in english Pesticides are one of the families of chemical products most widely used by man. They have been used above all to combat pests because of their effect on harvests and as vectors of transmissible diseases. Pesticides can be classified according to their use (insecticides, fungicides, herbicides, rati [...] cides…) or by their chemical family (organochlorates, organophosphates, carbamates, pyrethroids, Bipyridilium compounds, inorganic salts…). All of them are biocides, which normally implies a high toxicity for humans, which has been a cause for concern since the mid-XX century due to the widespread and indiscriminate use of these products. Exposure to pesticides can have effects that are acute, chronic and long-term. Some organochlorate compounds (such as DDT) were the first to be used in massive fumigations to fight malaria and have had to be banned because of their capacity for bioaccumulation and environmental persistence. The danger represented by the widespread presence of these agents has been demonstrated in numerous episodes of human toxic epidemics, producers of a high morbidity/mortality, described for nearly all chemical families: organochlorate insecticides and fungicides, organophosphate and carbamate insecticides, organomercurial fungicides and inorganic salts. These episodes have above all been caused through the ingestion of foodstuffs and in the occupational field. Other causes of health concern are their carcinogenic capacity and occasional reproductive alterations. The principal characteristics of some of the most relevant families are presented.

A., Ferrer.

332

REANASTOMOSIS TUBÁRICA POR VÍA LAPAROSCÓPICA  

Directory of Open Access Journals (Sweden)

Full Text Available Antecedentes: La esterilización tubárica se realiza en un número importante de pacientes jóvenes que por diferentes motivos desean posteriormente un nuevo embarazo, porcentaje que varía entre el 1,3-15%. Hasta ahora la cirugía ha sido la primera alternativa de tratamiento y gracias a la evolución de la técnica y el desarrollo de material quirúrgico, es posible de realizar por vía laparoscópica. Objetivos: Hacer una síntesis de la historia y técnicas utilizadas en la reanastomosis tubárica, los criterios de selección, el estudio preoperatorio, los factores pronósticos y comparar los resultados de la laparotomía y la laparoscopia. Métodos: Se realiza búsqueda en base de datos Medline PubMed usando las palabras clave: tubal anastomosis, reversal of esterilization, infertility, microsurgery, tubal esterilization; se seleccionan todas aquellas publicaciones tipo review de reanastomosis tubárica con microcirugía abierta y todas aquellas de reanastomosis por vía laparoscópica. Resultados: La reanastomosis tubárica por laparotomía con técnica microquirúrgica tiene un éxito entre 60 y 90% de tasas de embarazo. Las primeras publicaciones de la técnica laparoscópica no presentan resultados satisfactorios, sin embargo casuísticas más numerosas y especialmente las que muestran la técnica microlaparoscópica tienen resultados similares con todas las ventajas de la laparoscopia. Conclusión: La recanalización tubaria por vía laparoscopica es una técnica factible, probada y exitosa, alternativa a la laparotomía, sin embargo, para su implementación con resultados satisfactorios es necesario contar con equipamiento apropiado, experiencia previa en cirugía abierta y entrenamiento en cirugía endoscópicaBackground: Tubal ligation is used in a large number of young patients, who frequently want a new pregnancy, the percentage rate ranges from 1.3 to 15%. Until now the standard treatment has been the microsurgical tubal anastomosis through laparotomy, however, the development of new techniques and instruments allowed a laparoscopic approach for this surgery. Objectives: to make a synthesis of the hystory and operatory technics in laparoscopic tubal anastomosis to evaluate the inclusions criteria, the preoperative work-up, the factors of prognosis and to make a comparison between laparotomy and laparoscopy. Methods: We make a search in medline database (PubMed, with the key words: tubal anastomosis, reversal of sterilization, infertility, microsurgery, tubal sterilization, and selecting all of the review publications in microsurgical tubal reanastomosis by laparotomy and all of publications of the laparoscopic technic. Results: The microsurgical tubal anastomosis through laparotomy has a pregnancy rate ranging from 70% to 80%. The first publications whith the laparoscopic technic show dissapointed results, however, papers with bigger casuistics and with the microlaparoscopic technic show the same results that the open technic with all of the advantages of the laparoscopy. Conclusion: Laparoscopic tubal reversal is a feasible, tested and successful alternative to the traditional surgery performed by laparotomy, however, to achieve a successful outcome it is necessary a meticulous surgical technique, adequate equipment and extensive experience with tubal anastomosis by laparotomy and advances laparoscopic techniques

Juan Raúl Escalona M

2006-01-01

333

Impact of the total number of harvested lymph nodes after colon cancer resections on survival in patients without involved lymph node / Influencia del número total de ganglios analizados tras cirugía de resección en el cáncer de colon sobre la supervivencia en pacientes sin afectación ganglionar  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: English Abstract in spanish Introducción: el número total de ganglios analizados ha demostrado su influencia pronóstica en el cáncer de colon. Pueden existir grandes diferencias en el número de ganglios obtenidos por diferentes especialistas (cirujanos y anatomopatólogos). Objetivo: el objetivo del presente estudio fue analiza [...] r si, en nuestro medio, el número de ganglios analizados en pacientes con cáncer de colon clasificados como pN0 se relaciona también con la supervivencia. Material y métodos: estudio retrospectivo, con inclusión de 148 pacientes con adenocarcinoma de colon (pN0 de la clasificación TNM) intervenidos de forma programada con intención curativa entre 1 de enero de 1995 y 31 de diciembre de 2001. Se establecieron 3 grupos según el número de ganglios analizados ( 14 ganglios). Para el análisis de la supervivencia se utilizaron el método de Kaplan Meier y las gráficas CUSUM. Resultados: el número total de ganglios analizados fue 1.493 (media 10,1 ganglios por paciente). La supervivencia a 5 años fue del 63,0% en el grupo con 14 ganglios analizados (p Abstract in english Background: the total number of harvested lymph nodes has been demonstrated to be of prognostic significance for colon cancer. Differences can occur in the total number of harvested lymph nodes between different specialists (surgeons and pathologists). Objective: the aim of this study was to analyse [...] if, in our centre, the number of analysed lymph nodes in patients with colon cancer that are classified as pN0 is also related to survival. Material and methods: a retrospective study was designed, where 148 patients with colon adenocarcinoma (pN0 of TNM classification) who underwent elective surgery between 1 January 1995 and 31 December 2001, with curative intent were included. Three groups were created according to the number of analysed lymph nodes ( 14 lymph nodes). For survival analysis the Kaplan-Meier and CUSUM curves methods were used. Results: the total number of analysed lymph nodes was 1,493 (mean 10.1 lymph nodes per patient). The rate of 5-years survival was 63.0% in the group with 14 lymph nodes: 91.8% (p

M. I., Rivadulla-Serrano; D., Martínez-Ramos; M., Armengol-Carrasco; J., Escrig-Sos; J. M., Daroca-José; G. A., Paiva-Coronel; C., Fortea-Sanchís; J. L., Salvador-Sanchis.

2010-05-01

334

Impact of the total number of harvested lymph nodes after colon cancer resections on survival in patients without involved lymph node Influencia del número total de ganglios analizados tras cirugía de resección en el cáncer de colon sobre la supervivencia en pacientes sin afectación ganglionar  

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Full Text Available Background: the total number of harvested lymph nodes has been demonstrated to be of prognostic significance for colon cancer. Differences can occur in the total number of harvested lymph nodes between different specialists (surgeons and pathologists. Objective: the aim of this study was to analyse if, in our centre, the number of analysed lymph nodes in patients with colon cancer that are classified as pN0 is also related to survival. Material and methods: a retrospective study was designed, where 148 patients with colon adenocarcinoma (pN0 of TNM classification who underwent elective surgery between 1 January 1995 and 31 December 2001, with curative intent were included. Three groups were created according to the number of analysed lymph nodes ( 14 lymph nodes. For survival analysis the Kaplan-Meier and CUSUM curves methods were used. Results: the total number of analysed lymph nodes was 1,493 (mean 10.1 lymph nodes per patient. The rate of 5-years survival was 63.0% in the group with 14 lymph nodes: 91.8% (p Introducción: el número total de ganglios analizados ha demostrado su influencia pronóstica en el cáncer de colon. Pueden existir grandes diferencias en el número de ganglios obtenidos por diferentes especialistas (cirujanos y anatomopatólogos. Objetivo: el objetivo del presente estudio fue analizar si, en nuestro medio, el número de ganglios analizados en pacientes con cáncer de colon clasificados como pN0 se relaciona también con la supervivencia. Material y métodos: estudio retrospectivo, con inclusión de 148 pacientes con adenocarcinoma de colon (pN0 de la clasificación TNM intervenidos de forma programada con intención curativa entre 1 de enero de 1995 y 31 de diciembre de 2001. Se establecieron 3 grupos según el número de ganglios analizados ( 14 ganglios. Para el análisis de la supervivencia se utilizaron el método de Kaplan Meier y las gráficas CUSUM. Resultados: el número total de ganglios analizados fue 1.493 (media 10,1 ganglios por paciente. La supervivencia a 5 años fue del 63,0% en el grupo con 14 ganglios analizados (p < 0,01. La influencia pronóstica se mantuvo en el análisis multivariante. Conclusión: en nuestro medio, la obtención de un mayor número de ganglios analizados se relaciona con una mayor supervivencia a los 5 años en pacientes con cáncer de colon clasificados como pN0. Parece razonable recomendar la obtención de tantos ganglios como sea posible en este tipo de cirugía y no recomendar un número mínimo de ganglios que se deberían analizar.

M. I. Rivadulla-Serrano

2010-05-01

335

Rickettsiae, protozoa, and opisthokonta/metazoa.  

Science.gov (United States)

Rhizobiales (formerly named Rickettsiales) cause in rare instances meningitis and meningovasculitis, respectively. In case of history of exposure, infection by Rhizobiales needs to be considered since both diagnosis and therapy may be extremely difficult and pathogen-specific. The same applies to protozoa; in this chapter, Babesia species, free-living amoebae and Entamoeba histolytica infection, including severe meningitis and brain abscess, infection by Trypanosoma species (South American and African trypanosomiasis) are discussed with respect to history, epidemiology, clinical signs, and symptoms as well as differential diagnosis and therapy. Parasitic flatworms and roundworms, potentially able to invade the central nervous system, trematodes (flukes), cestodes (in particular, Cysticercus cellulosae), but also nematodes (in particular, Strongyloides spp. in the immunocompromised) are of worldwide importance. In contrast, filarial worms, Toxocara spp., Trichinella spp., Gnathostoma and Angiostrongylus spp. are seen only in certain geographically confined areas. Even more regionally confined are infestations of the central nervous system by metazoa, in particular, tongue worms (=arthropods) or larvae of flies (=maggots). The aim of this chapter is (1) to alert the neurologist to these infections, and (2) to enable the attending emergency neurologist to take a knowledgeable history, with an emphasis on epidemiology, clinical signs, and symptoms as well as therapeutic management possibilities. PMID:24365428

Schmutzhard, Erich; Helbok, Raimund

2014-01-01

336

Rickettsia felis in Ctenocephalides spp. Fleas, Brazil  

OpenAIRE

In June 2000, suspected cases of Brazilian spotted fever (BSF) occurred in Coronel Fabriciano Municipality, Minas Gerais State, Brazil. Pooled fleas collected near two fatal cases contained rickettsial DNA. The nucleotide sequence alignment of the 391-bp segment of the 17-kDa protein gene showed that the products were identical to each other and to the R. felis 17-kDa gene, confirming circulation of R. felis in Brazil.

Oliveira, Riva P.; Galva?o, Ma?rcio A. M.; Mafra, Claudio L.; Chamone, Chequer B.; Calic, Simone B.; Silva, Sergio U.; Walker, David H.

2002-01-01

337

Mortalidad infantil por malformaciones congénitas  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish RESUMEN Se realizó un estudio descriptivo retrospectivo del total de defunciones de los menores de un año ocurridas en la provincia Cienfuegos durante 1989-98. El dato primario fue recogido del registro de fallecidos del Departamento de Estadística del CPHE teniéndose en cuenta las siguientes variab [...] les: peso al nacer, municipio, tipo de malformación, edad del fallecimiento, causas de muertes y dirección para lo cual se realizó la distribución espacial utilizándose el programa MAPINFO 4.1, a partir de las tasas específicas por asentamiento. Además, se aplicó un índice de distribución mediante el cual obtuvimos los asentamientos con mayor peso de la mortalidad por anomalías en menores de 1 año, con respecto a la mortalidad total, el análisis se realizó mediante el programa EPINFO encontrándose que la tendencia de las defunciones es descendente y solo representan el 30 % del total de fallecidos por todas las causas, encontramos la mayor afectación en 3 municipios donde existen 21 asentamientos con tasas en el rango de 6,99 a 9,99. Abstract in english SUMMARY A descriptive and retrospective study of the total of deaths ocurred among children under 1 in the province of Cienfuegos from 1989 to 1998 was conducted. The primary data were taken from the registry of deaths of the Statistics Department of the Provincial Center of Hygiene and Epidemiology [...] , taking into account the following variables: birth weight, municipality, type of malformation, age on dying, causes of death and address. To this end, a spatial distribuion was made by using the MAPINFO 4.1 program, based on the specific rates by settlement. It was also applied a distribution index by which we obtained those settlements with higher mortality rate from abnormalities in children under 1. As regards total mortality, the analysis was made by using the EPINFO program. It was found that the trend of deaths was descending and represented only 30% of the total of deaths from all causes. The highest affectation was observed in 3 municipalities, where there were 21 settlements with rates fluctuating from 6.99 to 9.99.

Marta, Luján Hernández; Gloria, Fabregat Rodríguez.

2001-04-01

338

Mortalidad infantil por malformaciones congénitas  

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Full Text Available RESUMEN Se realizó un estudio descriptivo retrospectivo del total de defunciones de los menores de un año ocurridas en la provincia Cienfuegos durante 1989-98. El dato primario fue recogido del registro de fallecidos del Departamento de Estadística del CPHE teniéndose en cuenta las siguientes variables: peso al nacer, municipio, tipo de malformación, edad del fallecimiento, causas de muertes y dirección para lo cual se realizó la distribución espacial utilizándose el programa MAPINFO 4.1, a partir de las tasas específicas por asentamiento. Además, se aplicó un índice de distribución mediante el cual obtuvimos los asentamientos con mayor peso de la mortalidad por anomalías en menores de 1 año, con respecto a la mortalidad total, el análisis se realizó mediante el programa EPINFO encontrándose que la tendencia de las defunciones es descendente y solo representan el 30 % del total de fallecidos por todas las causas, encontramos la mayor afectación en 3 municipios donde existen 21 asentamientos con tasas en el rango de 6,99 a 9,99.SUMMARY A descriptive and retrospective study of the total of deaths ocurred among children under 1 in the province of Cienfuegos from 1989 to 1998 was conducted. The primary data were taken from the registry of deaths of the Statistics Department of the Provincial Center of Hygiene and Epidemiology, taking into account the following variables: birth weight, municipality, type of malformation, age on dying, causes of death and address. To this end, a spatial distribuion was made by using the MAPINFO 4.1 program, based on the specific rates by settlement. It was also applied a distribution index by which we obtained those settlements with higher mortality rate from abnormalities in children under 1. As regards total mortality, the analysis was made by using the EPINFO program. It was found that the trend of deaths was descending and represented only 30% of the total of deaths from all causes. The highest affectation was observed in 3 municipalities, where there were 21 settlements with rates fluctuating from 6.99 to 9.99.

Marta Luján Hernández

2001-04-01

339

Miotoxicidade por organofosforados Organophosphate myotoxicity  

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Full Text Available Os organofosforados são um grupo de compostos químicos amplamente utilizados em agropecuária como inseticidas, ocasionando intoxicações acidentais em animais e humanos, e mesmo sendo utilizados em tentativas de suicídio. A toxicidade desses produtos decorre sobretudo de insuficiência cárdio-respiratória por compromentimento do sistema nervoso autônomo. Sabe-se que alguns destes compostos induzem em animais de experimentação e em humanos, uma miopatia caracterizada por degeneração de células musculares, comprometendo sobretudo a musculatura respiratória. Baseado no fato de que este comprometimento contribui para a piora da função respiratória, propõe-se um protocolo de avaliação rotineira de miotoxicidade por compostos organofosforados, através de uma bateria mínima e suficiente de colorações e reações histoquímicas para quantificação da necrose muscular. Utilizaram-se como modelo experimental, grupos de ratos albinos (Wistar intoxicados com o organofosforado paraoxon, com e sem antídotos (atropina ou pralidoxima. Verificou-se nos grupos tratados com paraoxon e paraoxon mais atropina, necrose de fibras musculares no diafragma, que atingia em determinadas áreas até 15% das fibras. No grupo tratado com paraoxon mais pralidoxima, a necrose foi mínima, evidenciando o papel mioprotetor deste último antídoto.Organophosphates comprise a group of chemical compounds extensively used in farming as insecticides, which cause accidental poisoning in animals and men and are also used in suicide attempts. The toxicity of these compounds is due especially to the cardiac and respiratory impairment in consequence of autonomic nervous system disorders. However, it is known that some of these products induce a myopathy in experimental animals and humans. This myopathy is characterized by muscle cell degeneration, involving above all the respiratory muscles. Based on the fact that this involvement certainly enhances the respiratory impairment, this study offers an experimental method for routine evaluation of organophosphate myotoxicity, using a minimal and sufficient battery of stains and histochemical reactions, for muscle necrosis quantification. For this purpose, albino rats (Wistar treated with the organophosphate paraoxon, were used both with and without antidotes (atropine or pralidoxime. Muscle fiber necrosis in the diaphragm of the rats treated with paraoxon or paraoxon and atropine, that affected about 15% of the fibers in some areas, was detected. In the group treated with paraoxon and pralidoxime, a minimal necrosis was seen, revealing a protective role of this later antidote during the development of myopathy.

Maria J. Cavaliere

1996-06-01

340

Neumonía lipoidea por exposición laboral  

Scientific Electronic Library Online (English)

Full Text Available SciELO Costa Rica | Language: Spanish Abstract in spanish La neumonía lipoidea es una condición poco común que resulta de la presencia de lípidos en el interior del espacio alveolar. Según la fuente de los lípidos se ha clasificado en endógena y exógena y esta a su vez según la presentación en aguda y crónica. La neumonía lipoidea exógena se produce por as [...] piración o inhalación de sustancias oleosas, suele presentarse en pacientes con predisposición a broncoaspiración por alteraciones anatómicas o neurológicas, sin embargo se puede presentar en pacientes sanos que están expuestos a este tipo de sustancias y suele ser en el medio laboral. La fisiopatología es secundaria a una respuesta de cuerpo extraño que termina en fibrosis pulmonar. Los síntomas que produce al igual que manifestaciones al examen físico son inespecíficos y los hallazgos en los exámenes de laboratorio y gabinete también lo son, esto hace que sea una patología poco diagnosticada; sin embargo, haciendo una buena historia clínica e historia laboral se puede sospechar su diagnóstico en el caso de los médicos tratantes o establecer una relación de causalidad en el caso del médico forense. Presentación de caso: Se expone el caso de masculino de 34 años de edad referido por un caso de riesgo laboral, el mismo sin antecedentes patológicos conocidos, quien estuvo expuesto al humo del vehículo que conducía, posteriormente inicia con síntomas respiratorios inespecíficos y finalmente es diagnosticado con neumonía lipoidea. Abstract in english The lipoid pneumonia is an uncommon condition that results from the presence of lipids within the alveolar space. According to the source of lipids has been classified into endogenous and exogenous, and this in turn according to the presentation in acute and chronic. Exogenous lipoid pneumonia is ca [...] used by inhalation or aspiration of oily substances, usually seen in patients predisposed to aspiration for anatomical or neurological disorders, but also can occur in healthy patients who are exposed to these substances and are usually cases of occupational exposure. The pathophysiology is secondary to a foreign body response that ends in pulmonary fibrosis. Symptoms and physical examinations findings are nonspecific as well as laboratory and cabinet tests, this makes it a disease underdiagnosed; however, doing a good history and labor history may suspect the diagnosis in the case of treating physicians or establish a causal link if the case of the forensic medical. Case presentation: Male 34 years of age are exposed, referred by a case of occupational risk, the same with no known medical history, who was exposed to the vehicle driven smoke then begins with nonspecific respiratory symptoms and finally is diagnosed with lipoid pneumonia.

Ana Elena, Núñez Ocampo.

2014-12-01

341

Intoxicación por drogas Drug poisoning  

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Full Text Available El consumo de drogas ilegales en nuestro país ha experimentado un notable cambio en los últimos años, relegando a la heroína e incorporando la cocaína, los derivados anfetamínicos como el "éxtasis" (MDMA, el "éxtasis líquido" (GHB y, en menor medida, la ketamina. Se lleva a cabo una revisión de la intoxicación aguda por opiáceos y de su tratamiento en los servicios de urgencias, teniendo en cuenta el descenso progresivo de los casos que se presentan con el advenimiento de nuevas formas de administración, así como la presencia de nuevas drogas adictivas que han dado lugar a un desplazamiento en los hábitos de consumo. Se expone la intoxicación por cocaína haciendo referencia a la clínica, el diagnóstico y el tratamiento. Se realiza una revisión sobre el cannabis y sus derivados, la historia de su consumo y preparaciones utilizadas, los efectos que producen en los distintos sistemas del organismo y sus principales mecanismos de acción. Por último se comentan los efectos del LSD y de las setas alucinógenas.A review is made of acute poisoning by opiates and its treatment in the emergency services, bearing in mind the progressive decline in the number of cases presented with the arrival of new forms of their administration, as well as the presence of new addictive drugs that have resulted in a shift in consumption habits. Reference is also made to the way in which the different types of existing substances originated, with the aim of achieving a better understanding of their use and in order to administer the most suitable treatment when poisoning occurs. Cocaine poisoning is discussed, with reference to its clinical picture, diagnosis and treatment. The consumption of illegal drugs in our country has undergone a notable change in recent years, with heroin being relegated and the incorporation of cocaine, amphetamine derivatives such as "ecstasy" (MDMA, "liquid ecstasy" (GHB and, to a lesser extent, ketamine. A review is made of cannabis and its derivates, from the history of its consumption and the preparations employed to the effects produced in the different bodily systems. A brief explanation is also given of its metabolites and its principal mechanisms of action. Finally, we comment on the effects of LSD and hallucinogenic mushrooms.

I. Gainza

2003-01-01

342

DIOSCÓRIDES RESCATADO POR LOS ÁRABES  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish La historia oficial afirma que Occidente heredó directamente el legado cultural de Grecia y Roma. Pero si la mayoría de los manuscritos griegos y latinos fueron destruidos a partir del saqueo de la Biblioteca de Alejandría en 391, los archivos de Roma fueron devastados en sendas ocasiones entre 410 [...] y 476 por visigodos y hérulos, y los escasos vestigios clásicos que quedaban en Atenas fueron arrasados por Justiniano I en 529, ¿cuál fue la conexión que logró transmitir esa literatura y pudo ser aprovechada en un período anterior al renacimiento? La salvación de las piezas literarias se realizó durante ochocientos afios de activa y responsable tarea de recopilación por parte de los árabes durante la línea de tiempo que oscila entre 650-1450 en la que fueron rescatados, traducidos y retransmitidos hacia los cuatro puntos cardinales. Los científicos árabes, al igual que Arquímedes o Herón, nunca se separaron del saber empírico, por el contrario, lo profundizaron. El movimiento científico árabe no sólo interpretó el saber de los antiguos sino desarrolló una nueva ciencia donde la razón sustentaba a la fe y viceversa. Esta fue la base esencial que permitió construir el Renacimiento y la Ilustración, que a su vez posibilitaron la Modernidad. En este contexto, fue de capital importancia la traducción al árabe de la obra de Dioscórides ya que no sólo fue de gran utilidad para la farmacología y medicina en el mundo musulmán sino que a través de éste las recetas del médico griego de Nerón reingresaron a la Europa latina donde fueron recibidas como una panacea. Abstract in english Official history affirms that the West directly inherited the cultural legacy of Greece and Rome. But if most of the Greek and latin manuscripts they were destroyed at the sacking of the Library of Alexandria in 391, the archives of Rome were devastated in several occasions between 410 and 476 by Vi [...] sigoth and heruls, and the little classic vestiges that were in Athens were devastated by Justinian in 529, which was the connection that managed to transmit that Literature and could be taken advantage of it in a period previous to the Renaissance? The salvation of the literary mastepieces was made during eight hundred years od active and responsible task of compilation by the Arabs during the time line that oscillates between 650-1450 in that were rescued, translated and relayed towards the four cardinal points. The Arab scientists, like Archimedes or Hero, never separated of the empirical knowledge, on the contrary, they deepened it. The Arab scientific movement not only interpreted the knowledge of the old ones but it developed the new science where the reason sustained the faith and vice versa. This was the essential base that allowed to construct to the Renaissance and the Illustration, that made possible Modernity as well. In this context, it was of capital importance the translation to the Arab of the work of Dioscorides since it was not only very useful for the pharmacology and medicine in the Muslim World but that through this one the recipes of the Greek physician of Nero turned back to latin Europe where were received like a panacea.

Ricardo H, Elía.

343

Carcinomatosis peritoneal por tumor apendicular  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish INTRODUCCIÓN: el término Pseudomixoma Peritoneal es una entidad rara que hace referencia a la diseminación peritoneal de un tumor cuyas células producen gran cantidad de mucina. Suele proceder de un tumor apendicular perforado, y se distribuyen de una forma característica por la superficie peritonea [...] l. Aunque histológicamente puedan no mostrar agresividad, clínicamente sí lo son. MATERIAL Y MÉTODOS: presentamos el caso de una mujer diagnosticada de carcinomatosis peritoneal por un carcinoma apendicular. Fue tratada mediante cirugía y quimioterapia sistémica basada en 5FU. De esta forma conseguimos una larga supervivencia con un buen control sintomático. CONCLUSIONES: no existen tratamientos de elección para esta enfermedad. Basándose en su peculiar comportamiento clínico, algunos autores intentan una aproximación curativa, mediante cirugías agresivas y quimioterapia intraperitoneal. Se trata de un procedimiento complicado, no validado en ensayos clínicos. El caso que presentamos muestra que una opción razonable de tratamiento puede basarse en cirugía convencional y quimioterapia sistémica. Abstract in english PURPOSE: Mucinous ascites by mucus-producing tumour epithelial cells with peritoneum dissemination is a rare condition known as pseudomyoma peritonaei. In nearly all the cases the cause is a primary tumour of the appendix. It is an entity with multifocal peritoneal epithelial implants, distributed i [...] n a characteristic way. Although it can be not aggressive histologically, it is invariably fatal clinically. MATERIAL AND METHODS: We present the case of a woman diagnosed of peritoneal carcinomatosis by an appendicular carcinoma, who was treated by surgery and systemic chemotherapy based on 5-FU. With this approach we got a long survival with a good symptomatic control. CONCLUSION: There are no standard treatments for this disease. Based on its particular behaviour, the approach of some specialists is an aggressive surgery combined with intraperitoneal chemotherapy. However, it is a complex procedure, not validated by clinical studies. Our case shows that it can be achieved a good clinical control with conservative surgery and systemic chemotherapy.

A., Guerrero Zotano; J., Aparicio Urtasun; L., Pellín Ariño; J., Molina Saera; L., Palomar Abad; J., Ponce Lorenzo.

2005-04-01

344

Nefropatía por medios de contraste Nefropatía por medios de contraste  

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Full Text Available La nefropatía por medios de contraste es una causa importante de falla renal aguda en el ámbito hospitalario, generando altos costos al sistema de salud, morbilidad significativa y una mortalidad considerable. Se trata de un desorden iatrogénico consistente en un aumento absoluto (>0.5 mg o relativo (>25% de la creatinina sérica comparado con el basal, que ocurre dentro de las 24-48 horas después de la exposición a un medio de contraste, en ausencia de otra causa de lesión renal aguda. Son muchos los factores de riesgos que pueden predisponer para su presentación clínica. Se han experimentado una gran cantidad de estrategias preventivas, con el propósito de reducir la carga en términos de morbilidad y mortalidad derivadas de esta condición patológica, sin embargo, los resultados no son alentadores. Dentro de estas estrategias algunas han sido claramente inefectivas como el manitol, péptido atrial natriurético, teofilina, prostaglandina E y antagonistas de endotelina, en tanto que otros como la furosemida y dopamina son potencialmente dañinas. La evidencia actual soporta el uso de la infusión intravenosa de solución salina a 0.9% a 1 cc/kg/hora, 12 horas antes y 12 horas después de la aplicación del medio de contraste, haciendo una vigilancia estricta del balance hídrico. Por otra parte los datos obtenidos de los estudios clínicos para evaluar el efecto de la N-acetilcisteína en la prevención de la nefropatía por contraste, no son concluyentes (incluso contradictorios, sin embargo, la carencia de efectos secundarios y el potencial efecto benéfico, permite su uso rutinario como medida preventiva especialmente en pacientes de alto riesgo. Adicionalmente, si la premura del tiempo no permite la hidratación previa, la literatura soporta preferiblemente el uso de bicarbonato intravenoso isotónico una hora previa al procedimiento y continuar durante seis horas después del mismo.Nephropathy due to contrast means is an important cause of acute renal failure in the hospital environment, generating hight costs in the health care system, a significant morbidity and considerable mortality, it is considered to be an iatrogenic disorder that produces an absolute increase L (>0.5 mg or relative increase (>25% of serum creatinine as compared to the basal pattern and takes place within 24 to 48 hours after exposure to a contrast mean, in absence of another cause of acute renal insufficiency. There are many risk factors that can predispose to its clinical presentation. Many preventive strategies have been tried to reduce the load in terms of morbidity and mortality derived from this pathologic condition, however, the results are not encouraging. Some of these strategies have been clearly ineffective such as peptide manitol, natriuretic atrial peptide, theophiline, prostaglandin E and endotheline antagonists, whereas others such as furosemide and dopamine are potentially harmful. The evidence supports the use of the intravenous infusion of saline solution 0.9% a Icc/kg(hour, 12 hours before and 12 hours after giving the contrast mean, with a strict suveillance of the water balance. On the other hand, the data obtained from clinical trials to assess the effects of N-acetilcisteine in the prevention of nephropathy due to contrast means, are not concluding, (even contradictory; however, the lack of secondary effects and the potential beneficial effect, allows its use on a routine basis as a preventive measure especially in high risk patents. Additional to that, if the lack of time does not allow previous hydration, the literature rather supports the use of isotomic intravenous bicarbonate one hour before the procedure, and continuing during 6 hours after it.

Marcelo Aguirre Caicedo

2007-06-01

345

Nefropatía por medios de contraste / Nefropatía por medios de contraste  

Scientific Electronic Library Online (English)

Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish La nefropatía por medios de contraste es una causa importante de falla renal aguda en el ámbito hospitalario, generando altos costos al sistema de salud, morbilidad significativa y una mortalidad considerable. Se trata de un desorden iatrogénico consistente en un aumento absoluto (>0.5 mg) o relativ [...] o (>25%) de la creatinina sérica comparado con el basal, que ocurre dentro de las 24-48 horas después de la exposición a un medio de contraste, en ausencia de otra causa de lesión renal aguda. Son muchos los factores de riesgos que pueden predisponer para su presentación clínica. Se han experimentado una gran cantidad de estrategias preventivas, con el propósito de reducir la carga en términos de morbilidad y mortalidad derivadas de esta condición patológica, sin embargo, los resultados no son alentadores. Dentro de estas estrategias algunas han sido claramente inefectivas como el manitol, péptido atrial natriurético, teofilina, prostaglandina E y antagonistas de endotelina, en tanto que otros como la furosemida y dopamina son potencialmente dañinas. La evidencia actual soporta el uso de la infusión intravenosa de solución salina a 0.9% a 1 cc/kg/hora, 12 horas antes y 12 horas después de la aplicación del medio de contraste, haciendo una vigilancia estricta del balance hídrico. Por otra parte los datos obtenidos de los estudios clínicos para evaluar el efecto de la N-acetilcisteína en la prevención de la nefropatía por contraste, no son concluyentes (incluso contradictorios), sin embargo, la carencia de efectos secundarios y el potencial efecto benéfico, permite su uso rutinario como medida preventiva especialmente en pacientes de alto riesgo. Adicionalmente, si la premura del tiempo no permite la hidratación previa, la literatura soporta preferiblemente el uso de bicarbonato intravenoso isotónico una hora previa al procedimiento y continuar durante seis horas después del mismo. Abstract in english Nephropathy due to contrast means is an important cause of acute renal failure in the hospital environment, generating hight costs in the health care system, a significant morbidity and considerable mortality, it is considered to be an iatrogenic disorder that produces an absolute increase L (>0.5 m [...] g) or relative increase (>25%) of serum creatinine as compared to the basal pattern and takes place within 24 to 48 hours after exposure to a contrast mean, in absence of another cause of acute renal insufficiency. There are many risk factors that can predispose to its clinical presentation. Many preventive strategies have been tried to reduce the load in terms of morbidity and mortality derived from this pathologic condition, however, the results are not encouraging. Some of these strategies have been clearly ineffective such as peptide manitol, natriuretic atrial peptide, theophiline, prostaglandin E and endotheline antagonists, whereas others such as furosemide and dopamine are potentially harmful. The evidence supports the use of the intravenous infusion of saline solution 0.9% a Icc/kg(hour, 12 hours before and 12 hours after giving the contrast mean, with a strict suveillance of the water balance. On the other hand, the data obtained from clinical trials to assess the effects of N-acetilcisteine in the prevention of nephropathy due to contrast means, are not concluding, (even contradictory); however, the lack of secondary effects and the potential beneficial effect, allows its use on a routine basis as a preventive measure especially in high risk patents. Additional to that, if the lack of time does not allow previous hydration, the literature rather supports the use of isotomic intravenous bicarbonate one hour before the procedure, and continuing during 6 hours after it.

Marcelo, Aguirre Caicedo.

2007-06-01

346

Estudo comparativo das alterações clínicas e laboratoriais em canídeos mono-infectados com Leishmania infantum versus canídeos co-infectados com Leishmania infantum e com Babesia canis, Ehrlichia canis e/ou Rickettsia conorii  

OpenAIRE

A Leishmaniose Canina é uma doença sistémica grave, crónica, debilitante e potencialmente fatal, provocada por um protozoário difásico, a Leishmania infantum. È endémica em grande parte do território de Portugal Continental e nos últimos anos têm-se assistido a um aumento da sua prevalência nos cães. É transmitida ao hospedeiro vertebrado através da picada do insecto vector, Phlebotomus perniciosus. As doenças transmitidas por vectores constituem um grupo de doe...

Aguiar, Maria Catarina Costa Mendonc?a

2011-01-01

347

Tuberculosis miliar, ganglionar, pancreática y costal: Presentación clínica y revisión bibliográfica / Miliary, ganglionar, pancreatic and costal tuberculosis: Clinical presentation and bibliography review  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Se presenta un paciente de 42 años que ingresa en nuestro hospital con fiebre, dolor abdominal y patrón radiológico miliar. Tenía un aumento de amilasemia y amilasuria y de la lipasa sérica. El mantoux era negativo. En el estudio de TAC había engrosamiento costal, lesiones hepáticas focales múltiple [...] s y adenopatías necrosadas en zona peripancreática. Las biopsias transbronquiales y la punción pancreática mostraron lesiones granulomatosas necrotizantes, cultivándose M. tuberculosis complex en el broncoaspirado. El tratamiento específico normalizó todas las alteraciones pulmonares, costales y pancreáticas. Abstract in english A 42-year-old man was admitted to the hospital with fever, abdominal pain and miliary radiological pattern.The amylase and lipase in serum, and the amylase in urine were increased. The tuberculin skin test was negative. The thoracic and abdominal CT scan showed costal afectation, multiple focal hepa [...] tic lesions and large necrosing peripancreatic lymphadenopathies. The transbronchial biopsy and the pancreatic punction demostrated granulomatous necrotic lesions, and M. tuberculosis growed in the bronchial washing culture. The specific treatment normalized the pulmonary, costal and pancreatic lesions.

I., Inchaurraga Álvarez; A., Herrejón Silvestre; P., Plaza Valía; R., Blanquer Olivas.

2001-09-01

348

Linfomas não-Hodgkin extraganglionares: uma análise retrospectiva  

OpenAIRE

Na maioria dos linfomas não-Hodgkin (LNH), o envolvimento extra-ganglionar surge durante o curso da doença. Contudo alguns LNH têm origem em locais que não os gânglios linfáticos ou o baço, sendo designados por LNH extra-ganglionares. Este estudo tem como objectivo ilustrar as características clínico-patológicas dos doentes com LNH extra-ganglionares primários (LNH-EP). Foram avaliados 125 casos de LNH, dos quais 37 (30%) foram considerados LNH-EP. A proporção entre os sexos foi ...

Trindade, I.; Almeida, M.; Coimbra, F.; Portela, C.; Esperanc?a, S.; Marques, H.

2011-01-01

349

Concordancia entre el diagnóstico clínico y por laboratorio de fiebre por dengue y fiebre hemorrágica por dengue en Tabasco  

OpenAIRE

Objetivo. Determinar la concordancia del diagnóstico clínico de Fiebre por Dengue (FD) y Fiebre Hemorrágica por Dengue (FHD) y la presencia del virus del dengue confirmado por laboratorio en pacientes con síndromes febriles de Tabasco; además de la identificación del serotipo del dengue circulante, durante 2007. Material y métodos. El presente es un estudio observacional, transversal y descriptivo, donde se analizaron 3,718 muestras de pacientes con síndromes febriles, con el diagnós...

Borbolla-sala, Manuel E.; Rez-rojop, Isela E. Ju U. E.; Lez-alvarez, Norma Gonz U. E.; Eda Vanegas, Lorenzo Garc U.; Rrez, Olga E. Pi U. F. A-guti U. E.; Alfonso Rodr\\u00EDguez-Le\\u00F3n

2008-01-01

350

Intoxicación por alcoholes Alcohol intoxication  

Directory of Open Access Journals (Sweden)

Full Text Available La intoxicación etílica es la primera toxicomanía en muchos países del mundo. Afecta a todos los tramos de edad, en los dos sexos y en casi todos los grupos sociales. La mortalidad asociada sólo a la intoxicación etílica aguda es excepcional, pero puede ser un importante factor si coexiste con ingesta de otras drogas de abuso. Es responsable directo de más de la mitad de los accidentes de tráfico. El diagnóstico es fácil por la anamnesis y la clínica y se puede confirmar determinando el nivel de etanol en sangre. El tratamiento es de sostén, intentando proteger al paciente de complicaciones secundarias. El metanol o alcohol de quemar se utiliza como disolvente, encontrándose también como adulterante de bebidas alcohólicas. La intoxicación vía oral es la más frecuente. Oxidado en el hígado a través de la enzima alcohol deshidrogenasa, la toxicidad se debe a sus metabolitos, formaldehído y ácido fórmico. La clínica consiste fundamentalmente en cefalea, náuseas, vómitos, hipotensión y depresión del SNC. El nervio óptico es especialmente sensible pudiendo producirse una ceguera total e irreversible. El etilenglicol se utiliza como disolvente y anticongelante; la toxicidad se debe a la acumulación de sus metabolitos. La clínica incluye síntomas comunes con la intoxicación metílica. Puede ocurrir fallo renal por necrosis tubular y depósito de cristales de oxalato.Alcohol intoxication is the principal drug addiction in many countries of the world. It affects all age groups, both sexes and almost all social groups. Mortality associated with acute alcohol poisoning on its own is exceptional, but it can be an important factor if it coexists with recreational drugs. It is directly responsible for more than half of traffic accidents. Diagnosis is easy by means of anamnesis and clinical examination, and can be confirmed by determining the level of ethanol in the bloodstream. Supportive care is the best therapy in order to protect the patient from secondary complications. Methanol, or alcohol fuel, is used as a solvent, and can also be found as an adulterant of alcoholic drinks. Poisoning by oral means is the most frequent. Oxidized in the liver through dehydrogenase enzyme alcohol, toxicity is due to its metabolites, formaldehyde and formic acid. The clinical picture basically consists of cephalea, nausea, vomiting, hypotension and depression of the central nervous system. The optic nerve is especially sensitive, with total and irreversible blindness as a possible result. Ethylenglicol is used as a solvent and as an antifreeze; toxicity is due to an accumulation of its metabolites. The clinical picture includes symptoms that are held in common with methylalcohol intoxication. Kidney failure due to tubular necrosis and the deposit of oxalate crystals can occur.

J. Roldán

2003-01-01

351

Intoxicación por alcoholes / Alcohol intoxication  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish La intoxicación etílica es la primera toxicomanía en muchos países del mundo. Afecta a todos los tramos de edad, en los dos sexos y en casi todos los grupos sociales. La mortalidad asociada sólo a la intoxicación etílica aguda es excepcional, pero puede ser un importante factor si coexiste con inges [...] ta de otras drogas de abuso. Es responsable directo de más de la mitad de los accidentes de tráfico. El diagnóstico es fácil por la anamnesis y la clínica y se puede confirmar determinando el nivel de etanol en sangre. El tratamiento es de sostén, intentando proteger al paciente de complicaciones secundarias. El metanol o alcohol de quemar se utiliza como disolvente, encontrándose también como adulterante de bebidas alcohólicas. La intoxicación vía oral es la más frecuente. Oxidado en el hígado a través de la enzima alcohol deshidrogenasa, la toxicidad se debe a sus metabolitos, formaldehído y ácido fórmico. La clínica consiste fundamentalmente en cefalea, náuseas, vómitos, hipotensión y depresión del SNC. El nervio óptico es especialmente sensible pudiendo producirse una ceguera total e irreversible. El etilenglicol se utiliza como disolvente y anticongelante; la toxicidad se debe a la acumulación de sus metabolitos. La clínica incluye síntomas comunes con la intoxicación metílica. Puede ocurrir fallo renal por necrosis tubular y depósito de cristales de oxalato. Abstract in english Alcohol intoxication is the principal drug addiction in many countries of the world. It affects all age groups, both sexes and almost all social groups. Mortality associated with acute alcohol poisoning on its own is exceptional, but it can be an important factor if it coexists with recreational dru [...] gs. It is directly responsible for more than half of traffic accidents. Diagnosis is easy by means of anamnesis and clinical examination, and can be confirmed by determining the level of ethanol in the bloodstream. Supportive care is the best therapy in order to protect the patient from secondary complications. Methanol, or alcohol fuel, is used as a solvent, and can also be found as an adulterant of alcoholic drinks. Poisoning by oral means is the most frequent. Oxidized in the liver through dehydrogenase enzyme alcohol, toxicity is due to its metabolites, formaldehyde and formic acid. The clinical picture basically consists of cephalea, nausea, vomiting, hypotension and depression of the central nervous system. The optic nerve is especially sensitive, with total and irreversible blindness as a possible result. Ethylenglicol is used as a solvent and as an antifreeze; toxicity is due to an accumulation of its metabolites. The clinical picture includes symptoms that are held in common with methylalcohol intoxication. Kidney failure due to tubular necrosis and the deposit of oxalate crystals can occur.

J., Roldán; C., Frauca; A., Dueñas.

352

Tatuaje por amalgama. Reporte de un caso  

Directory of Open Access Journals (Sweden)

Full Text Available El tatuaje por amalgama se origina por el depósito en el tejido conectivo subepitelial de fragmentos de amalgama resultado de procedimientos iatrogénicos por parte del operador. La profundidad a la que se encuentren albergados los residuos de este material influye en la presentación clínica de las lesiones. Radiográficamente se pueden identificar los fragmentos mientras tengan diámetros razonables; histológicamente se pueden observar las partículas de amalgama como gránulos oscuros, sólidos e irregulares dispuesto entre los haces de colágeno y vasos sanguíneos. Este artículo refiere el caso clínico de un paciente que presentó pigmentación por amalgama en mucosa vestibular, originada por una porción de amalgama usada como material obturador en una apicectomía del 11 realizada con anterioridad. Teniendo en cuenta las consideraciones clínicas y radiográficas se optó por realizar una segunda apicectomía con obturación retrógrada con MTA del 11. Durante el procedimiento quirúrgico se cureteó y adelgazó la cara interna del colgajo mucoperióstico para tratar de disminuir el grado de pigmentación.

Luis Fang Mercado

2012-01-01

353

Suicidio por salto al vacío  

Scientific Electronic Library Online (English)

Full Text Available SciELO Costa Rica | Language: Spanish Abstract in spanish La tendencia al suicidio se asocia con problemas de soledad, abandono sicológico en la infancia, drogadicción, enfermedades graves, etc. Y en pacientes psiquiátricos es más común tras ser dados de alta. En Argentina los suicidios aumentaron en 25% de 1990 a 1992, y el 78 % de las víctimas fueron de [...] sexo masculino. La proporción por salto al vacío aumentó , especialmente en áreas urbanas. En 40 casos argentinos hubo introducción basal del fémur (25%), fractura del calcáneo en 17%, fracturas de pelvis o cuello del fémur en 7% y estallido hepático en 20%. Abstract in english Suicidal tendencies are associated with loneliness, lack of psychological attention during childhood, drug addiction, serious illness, etc., In psyciatric patients, it is more frequent shortly after they are dismissed from the hospital. In Argentina, the suicide rate increased by 25% from 1990 to 19 [...] 92, and 78% of the victims are males. The proportion that jumped to death increased, particularly inurban areas. In 40 Argentinian cases there was a basalpenetration of the femur (25%), fracture of the calcaneus bone (17%), fracture of pelvis o femur cervix (7%) and explosion of the liver (20%).

Braulio Ernesto, P. Linares; Félix Antonio, Bikic.

1995-05-01

354

Uveítis inducida por fármacos Drug induced uveitis  

OpenAIRE

Objetivo: Revisión de la literatura de los casos de uveítis inducida por fármacos. Métodos: Aplicación de los criterios de la OMS y de Naranjo para clasificar según lo publicado en la literatura los casos de uveítis inducida por fármacos en reacciones de causalidad cierta, probable y posible. Descripción de los cuadros clínicos de uveítis asociados a cada fármaco. Resultados: Uveítis de causalidad cierta son la causadas: por bifosfonatos, topiramato y metipranolol tópico. Uveít...

Cano Parra, J.; Di?az-llopis, M.

2005-01-01

355

Orlando Castellanos. La pasión por la radio  

OpenAIRE

Documento sonoro en el que se recogen entrevistas realizadas a Orlando Castellanos por Miguel Ángel de la Guardia, Miladys Ochoa, Fernando Rodríguez Sánchez (Ciego de Ávila), Ángel Ferrera, Boshmonar, Jaime Almiral, Radio Umbral de Santiago de Chile, Franco Carbón, Luis Toledo Sande y Estrella Díaz. Esta grabación pertenece a la colección Palabra Viva, desarrollada por el Centro Cultural Pablo de la Torriente Brau, a partir de las entrevistas realizadas por el periodista Orlando Cast...

Castellanos Molina, Orlando

2004-01-01

356

Tratamiento de lixiviados por medio de oxidación por peróxido de hidrógeno y ozono  

OpenAIRE

Blandón, Jaime Alberto and Marín, Luis Fernando (2004) Tratamiento de lixiviados por medio de oxidación por peróxido de hidrógeno y ozono. Pregrado thesis, Universidad Nacional de Colombia - Sede Manizales.

Blando?n, Jaime Alberto; Mari?n, Luis Fernando

2004-01-01

357

Ceratite bilateral por Acanthamoeba: relato de caso  

OpenAIRE

A ceratite por Acanthamoeba é uma infecção ocular grave que, apesar dos recentes progressos no diagnóstico e tratamento, ainda provoca prolongada morbidade e perda da acuidade visual. Relatamos um caso de ceratite bilateral por Acanthamoeba em usuário de lentes de contato, que é o primeiro caso descrito na literatura brasileira.

Obeid Wilson Nahmatallah; Araújo Rogério de; Vieira Luiz Antonio; Machado Marco Antonio de Campos

2003-01-01

358

Ceratite bilateral por Acanthamoeba: relato de caso  

Directory of Open Access Journals (Sweden)

Full Text Available A ceratite por Acanthamoeba é uma infecção ocular grave que, apesar dos recentes progressos no diagnóstico e tratamento, ainda provoca prolongada morbidade e perda da acuidade visual. Relatamos um caso de ceratite bilateral por Acanthamoeba em usuário de lentes de contato, que é o primeiro caso descrito na literatura brasileira.

Obeid Wilson Nahmatallah

2003-01-01

359

Endoftalmite por Candida albicans Candida albicans endophthalmitis  

OpenAIRE

O autor descreve os aspectos epidemiológicos, histopatológicos e clínicos da endoftalmite endógena por Candida albicans. Apresenta ainda novos métodos diagnósticos e opções terapêuticas utilizadas no tratamento das infecções fúngicas intra-oculares, por meio de revisão bibliográfica.The author describes epidemiological, histopathological and clinical aspects of endogenous Candida albicans endophthalmitis. He also presents new diagnostic methods and therapeutical options to t...

Pedro Duraes Serracarbassa; Patrícia Dotto

2003-01-01

360

Portable exhauster POR-007/Skid E and POR-008/Skid F storage plan  

International Nuclear Information System (INIS)

This document provides storage requirements for 1,000 CFM portable exhausters POR-O07/Skid E and POR-008/Skid F. These requirements are presented in three parts: preparation for storage, storage maintenance and testing, and retrieval from storage. The exhauster component identification numbers listed in this document contain the prefix POR-007 or POR-008 depending on which exhauster is being used