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Infección por rickettsia en capibaras (Hydrochoerus hydrochaeris) de São Paulo, Brasil: evidencia serológica de infección por Rickettsia bellii y Rickettsia parkeri Rickettsial infection in capybaras (Hydrochoerus hydrochaeris from São Paulo, Brazil: serological evidence for infection by Rickettsia bellii and Rickettsia parkeri  

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Introducción. En Brasil, los capibaras (Hydrochoerus hydrochaeris) son importantes huéspedes para garrapatas del género Amblyomma, las cuales transmiten rickettsiosis a humanos y animales. Por lo tanto, estos roedores pueden ser potenciales centinelas para detectar infección por rickettsia.
Objetivos. Este trabajo evaluó la infección por rickettsia en capibaras de diferentes regiones del estado de São Paulo, donde las rickettsiosis n...

Pacheco, Richard C.; Horta, Mauricio C.; Jonas Moraes-Filho; Ataliba, Alexandre C.; Adriano Pinter; Labruna, Marcelo B.

2007-01-01

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Caso probable de fiebre manchada ( Rickettsia felis) transmitida por pulgas / Probable case of flea-borne spotted fever ( Rickettsia felis )  

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Full Text Available SciELO Colombia | Language: Spanish Abstract in spanish Rickettsia felis es el agente etiológico de la fiebre manchada transmitida por pulgas, cuyo principal vector y reservorio es Ctenocephalides felis . Típicamente, la enfermedad se presenta como fiebre aguda asociada a cefalea, astenia, exantema máculo-papular generalizado y, en algunos casos, con esc [...] ara de inoculación. En los últimos años, R. felis ha venido adquiriendo un papel importante en la etiología del síndrome febril agudo, calificándola como una enfermedad emergente y subdiagnosticada. La inmunofluorescencia indirecta es actualmente el método diagnóstico de referencia. Sin embargo, esta técnica presenta limitaciones relacionadas con la reacción cruzada que existe entre las diferentes especies del género Rickettsia . En el presente reporte se describe el caso de un paciente de 16 años con síndrome febril agudo secundario a infección probable por R. felis . Abstract in english Rickettsia felis is the etiologic agent of flea-borne spotted fever, with Ctenocephalides felis as its main vector and reservoir. Typically, the disease presents as acute fever associated with headache, asthenia, generalized maculo-papular rash, and in some cases, an inoculation eschar. In recent ye [...] ars, R. felis has acquired an important role in the etiology of the acute febrile syndrome; it is indeed an emerging infectious disease, albeit underdiagnosed. Indirect immunofluorescence assay (IFA) is currently the reference diagnostic method. However, this technique has limitations related to the cross reactivity among different species of rickettsiae. Herein, we describe a case of a 16 year-old patient with an acute febrile syndrome secondary to probable infection with R. felis.

Faccini-Martínez, Álvaro A; Forero-Becerra, Elkin G; Cortés-Vecino, Jesús A; Polo-Teran, Luis J; Jácome, Jorge H; Vargas, Jimmy J; Valbuena, Gustavo; Hidalgo, Marylin.

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Clinical and laboratorial evidence of Rickettsia felis infections in Latin America / Evidência clínica e laboratorial de infecções por Rickettsia felis na América Latina  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese Depois da descoberta e caracterização inicial da Rickettsia felis em 1992 por Azad e cols, e à descrição subseqüente do primeiro caso de infecção humana em 1994, houveram duas comunicações de rickettsioses causadas por Rickettsia felis na América Latina. A primeira foi feita por Zavala-Velazquez e c [...] ols em 2000 no México. Em 2001, Raoult e cols descreveram a ocorrência de dois casos humanos de rickettsiose por Rickettsia felis no Brasil. Na presente discussão, esses dois artigos foram comparados, e depois da descrição dos principais sinais e sintomas, conclui-se que outros estudos são necessários, com a participação de um maior número de pacientes, para se estabelecer a verdadeira freqüência dos sinais clínicos e sintomas presentes nas rickettsioses por Rickettsia felis. Abstract in english After the discovery and initial characterization of Rickettsia felis in 1992 by Azad and cols, and the subsequent first description of a human case of infection in 1994, there have been two communications of human rickettsiosis cases caused by Rickettsia felis in Latin America. The first one was pub [...] lished in 2000 by Zavala-Velazquez and cols in Mexico. In 2001 Raoult and cols described the occurrence of two human cases of Rickettsia felis rickettsiosis in Brazil. In the present discussion these two articles were compared and after the description of the principal signs and symptoms, it was concluded that more studies are needed with descriptions of a greater number of patients to establish the true frequency of the clinical signs and symptoms present in Rickettsia felis rickettsiosis.

Galvão, Márcio Antônio Moreira; Mafra, Cláudio; Chamone, Chequer Buffe; Calic, Simone Berger; Zavala-Velazquez, Jorge E.; Walker, David Hughes.

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TUBERCULOSIS GANGLIONAR RETROPERITONEAL Y MESENTERICA: CASO CLINICO  

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Full Text Available Se describen las características del compromiso ganglionar por Mycobacterium tuberculosis en un paciente con SIDA en quien se demuestra alteraciones de linfonódulos retroperitoneales y mesentéricos en tomografía computada. Se discute las diferencias con el compromiso secundario a infección por Mycobacterium avium intracellulare y además el diagnóstico diferencial con otras formas de compromiso ganglionarThe changes of retroperitoneal lymph nodes in CT in a patient with AIDS are described. Differences with the compromise with M avium-intracellulare are discussed an also the differential diagnosis with other lymph node pathologies

Rafael Martínez F

2004-01-01

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??????: Rickettsia parkeri  

Full Text Available Bacteria Rickettsieae Rickettsia parkeri Rickettsia parkeri (species (AL)) DSMZ 784796 Rickettsia a parker (misspelling) NCBI 35792 Rickettsia parkeri (scientific name) NCBI 35792 Rickettsia pa

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Serological evidence of Rickettsia parkeri as the etiological agent of rickettsiosis in Uruguay / Evidência sorológica de Rickettsia parkeri como agente etiológico de rickettsiose no Uruguai  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese São relatados três novos casos humanos de rickettsiose no Uruguai. Os três casos clínicos apresentam manifestações clínicas semelhantes às descritas em casos de infecção por Rickettsia parkeri previamente relatados nos Estados Unidos, tais como: febre moderada ([...] a, escara de inoculação no sítio de fixação do carrapato, linfadenopatia regional e ausência de letalidade. Testes sorológicos de absorção de anticorpos com antígenos de R. parkeri e Rickettsia rickettsii, associados à reação de imunofluorescência indireta, sugerem que os pacientes de dois casos foram infectados por R. parkeri. Evidências clínicas e epidemiológicas, associadas com nossas análises sorológicas, sugerem que R. parkeri é o agente etiológico de casos humanos de febre maculosa no Uruguai, uma doença que tem sido reconhecida naquele país como rickettsiose cutâneo-ganglionar. Abstract in english We report three new rickettsiosis human cases in Uruguay. The three clinical cases presented clinical manifestations similar to previous reported cases of Rickettsia parkeri in the United States; that is mild fever ([...] l lymphadenopathy, and no lethality. Serological antibody-absorption tests with purified antigens of R. parkeri and Rickettsia rickettsii, associated with immunofluorescence assay indicated that the patients in two cases were infected by R. parkeri. Epidemiological and clinical evidences, coupled with our serological analysis, suggest that R. parkeri is the etiological agent of human cases of spotted fever in Uruguay, a disease that has been recognized in that country as cutaneous-ganglionar rickettsiosis.

Conti-Díaz, Ismael A.; Moraes-Filho, Jonas; Pacheco, Richard C.; Labruna, Marcelo B..

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??????: Rickettsia aeschlimannii  

Full Text Available Bacteria Rickettsieae Rickettsia aeschlimannii Rickettsia aeschlimannii (sp. nov. (VP)) DSMZ 784 780 Rickettsia MC16 (includes) NCBI 45262 Rickettsia aeschlimanii (misspelling) NCBI 45262 Rickettsia aeschlimanni (equivalent name) NCBI 45262 Ricketts

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??????: Rickettsia australis  

Full Text Available Bacteria Rickettsieae Rickettsia australis Rickettsia australis (species (AL)) DSMZ 784783 Rickettsia ttsia australis (scientific name) NCBI 787 Rickettsia australis Philip 1950, (synonym) NCBI 787

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??????: Rickettsia montanensis  

Full Text Available Bacteria Rickettsieae Rickettsia montanensis Rickettsia montanensis (sp. nov. (VL), nom. rev.) D SMZ 784795 Rickettsia montana Lackman et al. 1965 (synonym) NCBI 33991 R ickettsia montana (synonym) NCBI 33991 Rickettsia montanensis (scientific name) NCBI 33991 Rickettsi

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??????: Rickettsia felis  

Full Text Available Bacteria Rickettsieae Rickettsia felis Rickettsia felis (sp. nov. (VP)) DSMZ 784788 Rickettsia f elis Higgins et al. 1996 (authority) NCBI 42862 Rickettsia azadi (synonym) NCBI 42862 Rickettsia felis (scien tific name) NCBI 42862 Rickettsia felis Bouyer et al. 2001 emend. La Scola et al. 20

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??????: Rickettsia typhi  

Full Text Available Bacteria Rickettsieae Rickettsia typhi Rickettsia typhi (species (AL)) DSMZ 784806 Dermacentroxe nus typhi Wolbach and Todd 1920 (synonym) NCBI 785 Rickettsia mooseri Monteiro 1931 (synonym) NCBI 785 Dermacent roxenus typhi (synonym) NCBI 785 Rickettsia mooseri (synonym) NCBI 785 Rickettsia typhi (scien

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??????: Rickettsia honei  

Full Text Available Bacteria Rickettsieae Rickettsia honei Rickettsia honei (sp. nov. (VP)) DSMZ 784791 Rickettsia h onei (scientific name) NCBI 37816 Rickettsia honei Stenos et al. 1998 (synonym) NCBI 37816 Thai tick typhus rickettsia (synonym) NCBI 37816

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Clinical and laboratorial evidence of Rickettsia felis infections in Latin America Evidência clínica e laboratorial de infecções por Rickettsia felis na América Latina  

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After the discovery and initial characterization of Rickettsia felis in 1992 by Azad and cols, and the subsequent first description of a human case of infection in 1994, there have been two communications of human rickettsiosis cases caused by Rickettsia felis in Latin America. The first one was published in 2000 by Zavala-Velazquez and cols in Mexico. In 2001 Raoult and cols described the occurrence of two human cases of Rickettsia felis rickettsiosis in Brazil. In the present discussion the...

Márcio Antônio Moreira Galvão; Cláudio Mafra; Chequer Buffe Chamone; Simone Berger Calic; Zavala-velazquez, Jorge E.; David Hughes Walker

2004-01-01

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Serological evidence of Rickettsia parkeri as the etiological agent of rickettsiosis in Uruguay Evidência sorológica de Rickettsia parkeri como agente etiológico de rickettsiose no Uruguai  

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Full Text Available We report three new rickettsiosis human cases in Uruguay. The three clinical cases presented clinical manifestations similar to previous reported cases of Rickettsia parkeri in the United States; that is mild fever (São relatados três novos casos humanos de rickettsiose no Uruguai. Os três casos clínicos apresentam manifestações clínicas semelhantes às descritas em casos de infecção por Rickettsia parkeri previamente relatados nos Estados Unidos, tais como: febre moderada (< 40 ºC, mal-estar, cefaléia, exantema, escara de inoculação no sítio de fixação do carrapato, linfadenopatia regional e ausência de letalidade. Testes sorológicos de absorção de anticorpos com antígenos de R. parkeri e Rickettsia rickettsii, associados à reação de imunofluorescência indireta, sugerem que os pacientes de dois casos foram infectados por R. parkeri. Evidências clínicas e epidemiológicas, associadas com nossas análises sorológicas, sugerem que R. parkeri é o agente etiológico de casos humanos de febre maculosa no Uruguai, uma doença que tem sido reconhecida naquele país como rickettsiose cutâneo-ganglionar.

Ismael A. Conti-Díaz

2009-12-01

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Fiebre manchada por rickettsias en el Delta del Paraná: Una enfermedad emergente Rickettsial spotted fever in the Paraná Delta: An emerging disease  

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Full Text Available Se comunica un caso de fiebre manchada por rickettsia autóctono del delta del Paraná correspondiente a la provincia de Buenos Aires. Luego de cinco días de haber permanecido en una región cercana a la localidad de ingeniero Otamendi, partido de Campana, el paciente presentó un síndrome febril agudo caracterizado por hipertermia con escalofríos y sudoración, mialgias, cefalea, astenia y discreta odinofagia, seguido a las 72 horas por un exantema maculopapuloso congestivo con elementos purpúricos, de distribución universal. En la región preauricular izquierda se observaba una lesión papuloerosiva, producida cinco días antes de iniciada la fiebre por una garrapata adquirida en el lugar. El cuadro clínico remitió rápidamente con la administración de doxiciclina. Por inmunofluorescencia indirecta se identificaron anticuerpos reactivos contra el grupo de rickettsias causantes de fiebres manchadas (CDC, Atlanta, EE.UU.. Se realizan consideraciones sobre la especie de rickettsia, el vector involucrado y la posibilidad que la enfermedad fuera debida a Rickettsia parkeri.We describe a case of rickettsial spotted fever in the Paraná Delta region of Buenos Aires province in Argentina. The patient developed an acute febrile syndrome characterized by myalgias, headache, asthenia and moderate odynophagia, followed by a diffuse macular, papular, and purpuric exanthema. The patient had been bitten recently by a tick on the left preauricular region and an erosive papular lesion was evident at the bite site. An indirect immunofluorescence antibody assay identified antibodies reactive with spotted fever group rickettsiae in the patient's serum. The patient improved rapidly with doxycycline. Several considerations relating to the identity of the rickettsial species and tick vector are discussed, including the possibility that this patient's illness may have been caused by Rickettsia parkeri.

Alfredo Seijo

2007-12-01

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Fiebre manchada por rickettsias en el Delta del Paraná: Una enfermedad emergente / Rickettsial spotted fever in the Paraná Delta: An emerging disease  

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Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish Se comunica un caso de fiebre manchada por rickettsia autóctono del delta del Paraná correspondiente a la provincia de Buenos Aires. Luego de cinco días de haber permanecido en una región cercana a la localidad de ingeniero Otamendi, partido de Campana, el paciente presentó un síndrome febril agudo [...] caracterizado por hipertermia con escalofríos y sudoración, mialgias, cefalea, astenia y discreta odinofagia, seguido a las 72 horas por un exantema maculopapuloso congestivo con elementos purpúricos, de distribución universal. En la región preauricular izquierda se observaba una lesión papuloerosiva, producida cinco días antes de iniciada la fiebre por una garrapata adquirida en el lugar. El cuadro clínico remitió rápidamente con la administración de doxiciclina. Por inmunofluorescencia indirecta se identificaron anticuerpos reactivos contra el grupo de rickettsias causantes de fiebres manchadas (CDC, Atlanta, EE.UU.). Se realizan consideraciones sobre la especie de rickettsia, el vector involucrado y la posibilidad que la enfermedad fuera debida a Rickettsia parkeri. Abstract in english We describe a case of rickettsial spotted fever in the Paraná Delta region of Buenos Aires province in Argentina. The patient developed an acute febrile syndrome characterized by myalgias, headache, asthenia and moderate odynophagia, followed by a diffuse macular, papular, and purpuric exanthema. Th [...] e patient had been bitten recently by a tick on the left preauricular region and an erosive papular lesion was evident at the bite site. An indirect immunofluorescence antibody assay identified antibodies reactive with spotted fever group rickettsiae in the patient's serum. The patient improved rapidly with doxycycline. Several considerations relating to the identity of the rickettsial species and tick vector are discussed, including the possibility that this patient's illness may have been caused by Rickettsia parkeri.

Alfredo, Seijo; Marisa, Picollo; William, Nicholson; Christopher, Paddock.

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Tuberculose ganglionar: desafio diagnóstico / Tuberculous lymphadenitis: diagnostic challenge  

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Full Text Available SciELO Portugal | Language: Portuguese Abstract in portuguese A tuberculose ganglionar é a forma extrapulmonar mais comum de infecção por Mycobacterium tuberculosis. Não obstante, o mimetismo que mantém com outras patologias leva, comumente, a falha ou atraso na obtenção do diagnóstico correto. Os autores apresentam o caso clínico de tuberculose ganglionar num [...] a doente de 89 anos de idade, com múltiplas co-morbilidades, internada para esclarecimento de quadro constitucional caracterizado por febre e adenopatias cervicais de evolução indolente. Abstract in english Tuberculous lymphadenitis is the most common form of extrapulmonary Mycobacterium tuberculosis infection. Nevertheless, the mimicry that keeps with other pathologies leads, commonly, to failure or delay in obtaining a correct diagnosis. The authors present the case of tuberculous lymphadenitis in a [...] 89 years old female, with multiple co-morbidities, admitted with constitutional framework characterized by fever and cervical lymphadenopathy.

Miguel, Neno; Cláudia, Rocha; Dora, Sargento; Glória, Silva.

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Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay Rickettsia parkeri: patógeno rickettsial transmitido por garrapatas en áreas endémicas de rickettsiosis por fiebre manchada en el sur de Uruguay  

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At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human-biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in Uruguay. To address this question, 180 R. sanguineus from dogs and 245 A. triste from vegetation (flagging) collected in three endemic localitie...

Venzal, Jose? M.; Agustín Estrada-Peña; Aránzazu Portillo; Mangold, Atilio J.; Oscar Castro; Souza, Carlos G.; Fe?lix, Mari?a L.; Laura Pérez-Martínez; Sonia Santibánez; Oteo, Jose? A.

2012-01-01

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Fatal Brazilian spotless fever caused by Rickettsia rickettsii in a dark-skinned patient Febre maculosa brasileira sem exantema causada por Rickettsia rickettsii em um paciente de cor negra  

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Full Text Available Brazilian spotted fever (BSF is the most important and frequent rickettsial disease in Brazil. A fatal case of BSF is reported in a 32-year-old black man, who died of irreversible shock after five days of fever, severe headache and abdominal pain with no rash. Spleen, kidney and heart samples collected at autopsy were positive for Rickettsia rickettsii by PCR and sequencing. The authors emphasize the need for a high index of diagnostic suspicion for spotted fever in black patients. Absence of a skin rash should not dissuade clinicians from considering the possibility of BSF and initiating empirical therapy.Febre maculosa brasileira (FMB é a mais importante e frequente doença rickettsial no Brasil. Relatamos um caso fatal de FMB em um homem negro de 32 anos de idade que morreu de choque irreversível após cinco dias de febre, cefaléia intensa, dor abdominal, e sem evidência de exantema. Amostras de baço, rim e coração coletadas na necropsia foram positivas para Rickettsia rickettsii por PCR e sequenciamento. Os autores ressaltam a necessidade de um alto índice de suspeita diagnóstica para febre maculosa em pacientes negros. Ausência de exantema não deve dissuadir os clínicos de considerar a possibilidade de FMB e iniciar a terapêutica empírica.

Alexsandra Rodrigues de Mendonça Favacho

2011-06-01

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Fatal Brazilian spotless fever caused by Rickettsia rickettsii in a dark-skinned patient / Febre maculosa brasileira sem exantema causada por Rickettsia rickettsii em um paciente de cor negra  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese Febre maculosa brasileira (FMB) é a mais importante e frequente doença rickettsial no Brasil. Relatamos um caso fatal de FMB em um homem negro de 32 anos de idade que morreu de choque irreversível após cinco dias de febre, cefaléia intensa, dor abdominal, e sem evidência de exantema. Amostras de baç [...] o, rim e coração coletadas na necropsia foram positivas para Rickettsia rickettsii por PCR e sequenciamento. Os autores ressaltam a necessidade de um alto índice de suspeita diagnóstica para febre maculosa em pacientes negros. Ausência de exantema não deve dissuadir os clínicos de considerar a possibilidade de FMB e iniciar a terapêutica empírica. Abstract in english Brazilian spotted fever (BSF) is the most important and frequent rickettsial disease in Brazil. A fatal case of BSF is reported in a 32-year-old black man, who died of irreversible shock after five days of fever, severe headache and abdominal pain with no rash. Spleen, kidney and heart samples colle [...] cted at autopsy were positive for Rickettsia rickettsii by PCR and sequencing. The authors emphasize the need for a high index of diagnostic suspicion for spotted fever in black patients. Absence of a skin rash should not dissuade clinicians from considering the possibility of BSF and initiating empirical therapy.

Favacho, Alexsandra Rodrigues de Mendonça; Rozental, Tatiana; Calic, Simone Berger; Scofield, Maria Aparecida Mota; Lemos, Elba Regina Sampaio de.

 
 
 
 
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Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná  

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Full Text Available INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG. Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene. RESULTS: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6% were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. CONCLUSIONS: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans.INTRODUÇÃO: A febre maculosa é uma zoonose emergente causada por espécies de Rickettsia do grupo febre maculosa (GFM. Rickettsia rickettsii é o principal agente etiológico da febre maculosa brasileira (FMB e é transmitida por Amblyomma spp. MÉTODOS: Com o objetivo de obter informações sobre GFM Rickettsiae no Parque Municipal Arthur Thomas em Londrina, PR, carrapatos de vida livre e de capivaras foram coletados, assim como amostras de sangue das pessoas que trabalham no parque. A. dubitatum e A. cajennense foram submetidos à PCR em pools para analises de Rickettsia spp. gltA (citrate synthase gene. RESULTADOS: Todos os pools de carrapatos analizados foram negativos. Soros de humanos foram testados pela imunofluorescência indireta com antigenos de R. rickettsii e R. parkeri. Entre os 34 soros analisados, 7 (20,6% foram positivos para R. rickettsii. Destes, quatro apresentaram títulos iguais a 64, dois iguais a 128 e um, igual a 256, mas nenhum soro reagiu com R. parkeri. Não houve nenhuma associação, estatisticamente significante, entre as variáveis analisadas no questionário epidemiológico fornecido às pessoas que participaram da pesquisa. CONCLUSÕES: Os estudos sorológicos sugerem a presença de alguma Rickettsiae relacionada ao GFM que poderiam estar infectando a população humana estudada. Entretanto, as análises dos carrapatos foram inconclusivas para determinar qual espécie poderia estar envolvida na transmissão para os humanos.

Roberta Santos Toledo

2011-06-01

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Surveillance of Rickettsia sp. infection in capybaras (Hydrochoerus hydrochaeris) a potential model of epidemiological alert in endemic areas Vigilancia de la infección por Rickettsia sp. en capibaras (Hydrochoerus hydrochaeris) un modelo potencial de alerta epidemiológica en zonas endémicas  

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Introduction. Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rodents are usually parasitized by the tick vector, Amblyomma cajennense, the main vector of rickettsioses in humans and animals in South America. Capybaras can be used as sentinels in detection of circulation of rickettsiae.
Objective. Antibodies to rickettsiae of spotted fever group were detected in capybaras in a rural area of Cordoba Province, northern Colombia.

Jorge Miranda; Verónica Contreras; Yesica Negrete; Labruna, Marcelo B.; Salim Mattar

2011-01-01

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Larvas migrans ganglionar: Presentación de un caso  

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Full Text Available Las larvas migrans visceral cuya causa radica en la infestación con larvas de toxocara canis o cati, ocurre más frecuentemente en niños menores de 10 años. Se presenta el caso de un niño de 8 años de edad en el cual se diagnosticó larvas migrans ganglionar. Se comentan aspectos etioepidemiológicos de la enfermedad, su cuadro clínico y evolución. Se hace énfasis en las medidas higiénicas sanitarias de control y manipulación de excretas de animales domésticos, en este caso de perros y gatos.Visceral larvae migrans caused by the infestation with larvae of toxocara canis or cati are more frequent among children under 10. The case of an 8-year-old boy who was diagnosed ganglionar larva migrans is presented. Comments are made on some etioepidemiological aspects of the disease, as well as on his clinical picture and evolution. Emphasis is made on the hygienic and sanitary measures of control and manipulation of stools from pegs as dogs and cats. Las larvas migrans visceral cuya causa radica en la infestación con larvas de toxocara canis o cati, ocurre más frecuentemente en niños menores de 10 años. Se presenta el caso de un niño de 8 años de edad en el cual se diagnosticó larvas migrans ganglionar. Se comentan aspectos etioepidemiológicos de la enfermedad, su cuadro clínico y evolución. se hace énfasis en las medidas higiénicas sanitarias de control y manipulación de excretas de animales domésticos, en este caso de perros y gatos.

María del Carmen Luis Álvarez

2003-04-01

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Study of infection by Rickettsiae of the spotted fever group in humans and ticks in an urban park located in the City of Londrina, State of Paraná, Brazil / Estudo da infecção por Rickettsias do grupo da febre maculosa em humanos e carrapatos de um parque urbano na Cidade de Londrina, Estado do Paraná  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese INTRODUÇÃO: A febre maculosa é uma zoonose emergente causada por espécies de Rickettsia do grupo febre maculosa (GFM). Rickettsia rickettsii é o principal agente etiológico da febre maculosa brasileira (FMB) e é transmitida por Amblyomma spp. MÉTODOS: Com o objetivo de obter informações sobre GFM Ri [...] ckettsiae no Parque Municipal Arthur Thomas em Londrina, PR, carrapatos de vida livre e de capivaras foram coletados, assim como amostras de sangue das pessoas que trabalham no parque. A. dubitatum e A. cajennense foram submetidos à PCR em pools para analises de Rickettsia spp. gltA (citrate synthase gene). RESULTADOS: Todos os pools de carrapatos analizados foram negativos. Soros de humanos foram testados pela imunofluorescência indireta com antigenos de R. rickettsii e R. parkeri. Entre os 34 soros analisados, 7 (20,6%) foram positivos para R. rickettsii. Destes, quatro apresentaram títulos iguais a 64, dois iguais a 128 e um, igual a 256, mas nenhum soro reagiu com R. parkeri. Não houve nenhuma associação, estatisticamente significante, entre as variáveis analisadas no questionário epidemiológico fornecido às pessoas que participaram da pesquisa. CONCLUSÕES: Os estudos sorológicos sugerem a presença de alguma Rickettsiae relacionada ao GFM que poderiam estar infectando a população humana estudada. Entretanto, as análises dos carrapatos foram inconclusivas para determinar qual espécie poderia estar envolvida na transmissão para os humanos. Abstract in english INTRODUCTION: Spotted fevers are emerging zoonoses caused by Rickettsia species in the spotted fever group (SFG). Rickettsia rickettsii is the main etiologic agent of Brazilian spotted fever (BSF) and it is transmitted by Amblyomma spp. ticks. METHODS: The study aimed to investigate SFG rickettsiae [...] in the Arthur Thomas Municipal Park in Londrina, PR, by collecting free-living ticks and ticks from capybaras and blood samples from personnel working in these areas. Samples from A. dubitatum and A. cajennense were submitted for PCR in pools to analyze the Rickettsia spp. gltA (citrate synthase gene). RESULTS: All the pools analyzed were negative. Human sera were tested by indirect immunofluorescence assay with R. rickettsii and R. parkeri as antigens. Among the 34 sera analyzed, seven (20.6%) were reactive for R. rickettsii: four of these had endpoint titers equal to 64, 2 titers were 128 and 1 titer was 256. None of the samples were reactive for R. parkeri. An epidemiological questionnaire was applied to the park staff, but no statistically significant associations were identified. CONCLUSIONS: The serological studies suggest the presence of Rickettsiae related to SFG that could be infecting the human population studied; however, analysis of the ticks collected was unable to determine which species may be involved in transmission to humans.

Toledo, Roberta Santos; Tamekuni, Katia; Silva Filho, Mauro de Freitas; Haydu, Valeska Bender; Pacheco, Richard Campos; Labruna, Marcelo Bahia; Dumler, John Stephen; Vidotto, Odilon.

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AFECTACIÓN GANGLIONAR EN PACIENTE CON ENDOMETRIOSIS PROFUNDA RECTOVAGINAL  

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Full Text Available Se describe un raro caso de endometriosis rectovaginal con compromiso ganglionar en mujer de 33 años. La presencia de tejido endometrial en ganglios linfáticos pélvicos es rara y ha sido confirmada en la literatura en mujeres que han sido sometidas a cirugía por endometriosis. La presencia de endometriosis en los ganglios linfáticos pélvicos es muy improbable que surja de novo y sugiere extensión de la enfermedad.A rare case of rectovaginal endometriosis with lymph node involvement is described in a 33-year-old patient. The presence of endometrial tissue in pelvic lymph nodes is rare and has been confirmed in the literature in subjects who underwent surgery for endometriosis. Involvement of pelvic lymph nodes by endometriosis seems unlikely to arise de novo and probably suggests lymphatic spread of the disease.

Israel Ortega Sánchez

2009-01-01

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Larvas migrans ganglionar: Presentación de un caso  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Las larvas migrans visceral cuya causa radica en la infestación con larvas de toxocara canis o cati, ocurre más frecuentemente en niños menores de 10 años. Se presenta el caso de un niño de 8 años de edad en el cual se diagnosticó larvas migrans ganglionar. Se comentan aspectos etioepidemiológicos d [...] e la enfermedad, su cuadro clínico y evolución. Se hace énfasis en las medidas higiénicas sanitarias de control y manipulación de excretas de animales domésticos, en este caso de perros y gatos. Abstract in english Visceral larvae migrans caused by the infestation with larvae of toxocara canis or cati are more frequent among children under 10. The case of an 8-year-old boy who was diagnosed ganglionar larva migrans is presented. Comments are made on some etioepidemiological aspects of the disease, as well as o [...] n his clinical picture and evolution. Emphasis is made on the hygienic and sanitary measures of control and manipulation of stools from pegs as dogs and cats. Las larvas migrans visceral cuya causa radica en la infestación con larvas de toxocara canis o cati, ocurre más frecuentemente en niños menores de 10 años. Se presenta el caso de un niño de 8 años de edad en el cual se diagnosticó larvas migrans ganglionar. Se comentan aspectos etioepidemiológicos de la enfermedad, su cuadro clínico y evolución. se hace énfasis en las medidas higiénicas sanitarias de control y manipulación de excretas de animales domésticos, en este caso de perros y gatos.

María del Carmen, Luis Álvarez; Martha, Moroño Guerrero; Raquel, Cabrera Panizo; Mabel, González Alemán; Milagros, Morales Leyva; Roberto, Silva Aguiar.

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Fisiología de la retina III. Mensaje visual en la segunda sinapsis: las células ganglionares  

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El sistema visual responde a cambios rápidos y lentos de la energía luminosa en función del tiempo, interpretando casi instantáneamente, la información de ese medio exterior variable. Esta sensibilidad temporal y espacial del sistema visual, está organizada precisamente por interacciones excitadoras e inhibidoras entre los elementos nerviosos del sistema visual aferente. Las células ganglionares reciben el flujo de información a través de las bipolares y amacrinas. Estas células, si...

2006-01-01

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First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro Primeira identificação de infecção natural por Rickettsia rickettsii no carrapato Rhipicephalus sanguineus no Rio de Janeiro  

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Full Text Available The Brazilian Spotted Fever (BSF is a zoonotic disease caused by Rickettsia rickettsii and transmitted by ticks of the genus Amblyomma, more frequently, Amblyomma cajennense. The aim of this paper was to report the first molecular detection of R. rickettsii on R. sanguineus naturally infected in Rio de Janeiro, Brazil. Ticks were collected from dogs in a rural region of Resende municipality, Rio de Janeiro State, Brazil (22º30'9.46"S, 44º42'44.29"WO, where occurred five human cases of BSF in 2006. The ticks were identified under a stereoscopic microscope and separated in pools by stages, species and sex. DNA extraction was carried out using QIAamp DNA Mini Kit (QIAGEN®. The DNA was submitted to PCR amplification using 04 set of primers: Rr190.70p/Rr190.602n (OmpA, 532bp, BG1-21/BG2-20 (OmpB, 650bp, Tz15/Tz16 (17 kDa protein-encoding gene, 246bp and RpCS.877p/RpCS.1258n (gltA, 381bp. PCR products were separated by electrophoresis on 1% agarose gels and visualized under ultraviolet light with ethidium bromide. PCR products of the expected sizes were purified by QIAquick® and sequenced by ABI PRISM®. The generated nucleotide sequences were edited with using Bioedit® software and compared with the corresponding homologous sequences available through GenBank, using Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov. It was confirmed R. rickettsii by sequencing of the material (GenBank FJ356230. The molecular characterization of R. rickettsii in the tick R. sanguineus emphasizes the role of dogs as carriers of ticks from the environment to home. Moreover, this result suggests that there is a considerable chance for active participation of R. sanguineus as one of tick species in the transmission of R. ricketsii to human being in the Brazilian territory.A Febre Maculosa Brasileira (FMB é uma zoonose causada por Rickettsia rickettsii e transmitida por carrapatos do gênero Amblyomma, mais freqüentemente pela espécie Amblyomma cajennense. Este trabalho tem como objetivo relatar a primeira detecção molecular de R. rickettsii em Rhipicephalus sanguineus naturalmente infectado no Rio de Janeiro, Brasil. Carrapatos foram coletados de cães, procedentes de uma região rural do município de Resende, estado do Rio de Janeiro, Brasil (22º30'9.46"S, 44º42'44.29"WO, onde ocorreram cinco casos humanos de FMB em 2006. Todos os carrapatos foram identificados segundo chave dicotômica, utilizando-se lupa estereoscópica e separados de acordo com estágio, espécie e sexo. Para a extração de DNA utilizou-se o kit comercial QIAamp DNA (QIAGEN ®. O DNA foi submetido à técnica de PCR utilizando 04 conjuntos de iniciadores para a amplificação dos genes: Rr190.70p/Rr190.602n (OmpA, 532bp, BG1-21/BG2-20 (OmpB, 650bp, Tz15/Tz16 (17 kDa gene que codifica a proteína, 246bp e RPCs .877p/RpCS.1258n (gltA, 381bp. Os produtos da PCR foram separados por eletroforese em gel agarose 1% corados com brometo de etídio e visualizados sob luz ultravioleta e, aqueles que apresentaram bandas amplificadas foram purificados utilizando-se o kit comercial QIAquick ® e seqüenciados pelo ABI PRISM®. As seqüências nucleotídicas foram geradas usando Bioedit®, editado em software e comparados os correspondentes homólogos com as sequências disponíveis através GenBank, utilizando Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov. Confirmou-se R. rickettsii (GenBank FJ356230 no seqüenciamento de apenas um espécime, adulto de carrapato R. sanguineus. A caracterização molecular de R. rickettsii em exemplar de carrapato R. sanguineus confirma que esta espécie pode ter importante papel na transmissão de R. rickettsii para humanos no território brasileiro.

Nathalie C. Cunha

2009-02-01

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Factores predictores de metástasis ganglionar en el carcinoma diferenciado de tiroides  

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Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish Antecedentes: son necesarios factores pronósticos confiables de metástasis ganglionar para adaptar el tratamiento quirúrgico inicial de pacientes con carcinoma diferenciado de tiroides. Objetivo: determinar la frecuencia y factores pronósticos asociados con metástasis ganglionar en pacientes operado [...] s por carcinoma diferenciado de tiroides. Lugar de apicación: práctica privada. Diseño: retrospectivo observacional. Pobación: entre enero de 2000 y agosto de 2010, a 600 pacientes con 639 tumores (39 bilaterales) se les realizó tiroidectomía total y linfadenectomía terapéutica sólo cuando se demostró metástasis por biopsia ganglionar. Método: revisión de historias clínicas e informes patológicos. Resutados: 145 enfermos (22.7 %) tuvieron ganglios histológicamente positivos. El análisis multivariado mostró que la edad menor de 45 años (p = 0.001), adenopatías palpables (p = 0.0001), multicentricidad (p = 0.005) e invasión extracapsular (p = 0.0001) fueron factores de riesgo independientes de metástasis ganglionar. Estos factores, en conjunto, tuvieron una alta especificidad (97 %)y una baja sensibilidad (40 %). Se encontraron metástasis en ganglios yugulares con ganglios centrales negativos ("skip" metástasis) en 29 casos (5.54 %). Concusiones: a pesar de que algunos de los factores estudiados tuvieron valor pronóstico, se requieren variables adicionales para definir mejor el manejo quirúrgico. Abstract in english Background: reliable prognostic factors of lymph node metástasis are needed to adapt initial surgical treatment of patients with differentiated thyroid carcinoma. Objetive: to determine the frequency and predictive factors associated with lymph node metástasis in patients operated on for differentia [...] ted thyroid carcinoma. Setting: prívate practice. Design: retrospective observational. Popuation: between January 2000 and August 2010, 600 patients with 639 tumours (39 bilateral) underwent total thyroidectomy and therapeutic neck dissection only when there was biopsy proved lymph node metástasis. Method: review of clinical records and pathological reports. Resuts: 145 patients (22.7 %) had histologically positive lymph nodes. Multivariate analysis showed that lessthan 45 years (p = 0.001), palpable adenopathy (p = 0.0001), multicentricity (p = 0.005) and extracapsular invasión (p = 0.0001) were independent risk factors of lymph node metástasis. These factors, together, had high specificity (97 %) but low sensibility (40 %). Metástasis in jugular lymph nodes with normal central nodes (skip metástasis) was found in 29 (5.54 %) cases. Concusions: even though some of the factors studied proved to be of prognostic valué, additional variables are needed to better define surgical management.

Jorge E, Falco; Alvaro, Otero Muñoz; Manuel R, Montesinos.

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Tuberculosis ganglionar en un adulto joven / Lymph node tuberculosis in a young adult  

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Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Se expone el caso clínico de un recluso de 34 años de edad, de procedencia rural, quien fue atendido por especialistas de neumología a causa de cuadro febril crónico, astenia, pérdida de peso, inapetencia y aumento de volumen no doloroso en la región lateral derecha del cuello. Los exámenes compleme [...] ntarios mostraron imágenes de adenomegalias en hilio hepático y epigastrio. La muestra extraída del ganglio cervical para biopsia reveló necrosis caseosa de origen tuberculoso, por lo cual se diagnosticó tuberculosis ganglionar con afectación de cadenas ganglionares superficiales y profundas; enfermedad muy poco frecuente con esas características en el territorio. Abstract in english The case of 34 year-old prisoner is reported, who lived in a rural area and was attended by pulmonologists because of chronic feverishness, asthenia, weight loss, loss of appetite and painless enlargement in the right lateral region of the neck. Complementary tests revealed lymphadenopathies in port [...] a of liver and epigastrium. Cervical node biopsy revealed tuberculous caseous necrosis, thus diagnosing as lymph node tuberculosis with involvement of superficial and deep lymph node chains; an infrequent disease with these characteristics in the territory.

Joaquín, López González; Sahily, Quintero Salcedo; Odalis, García Gómez; Haydée, Marrero Rodríguez; Odalis, López Soria.

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Rickettsia africae and Candidatus Rickettsia barbariae in Ticks in Israel.  

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Abstract. DNA of several spotted fever group rickettsiae was found in ticks in Israel. The findings include evidence for the existence of Rickettsia africae and Candidatus Rickettsia barbariae in ticks in Israel. The DNA of R. africae was detected in a Hyalomma detritum tick from a wild boar and DNA of C. Rickettsia barbariae was detected in Rhipicephalus turanicus and Rhipicephalus sanguineus collected from vegetation. The DNA of Rickettsia massiliae was found in Rh. sanguineus and Haemaphysalis erinacei, whereas DNA of Rickettsia sibirica mongolitimonae was detected in a Rhipicephalus (Boophilus) annulatus. Clinicians should be aware that diseases caused by a variety of rickettsiae previously thought to be present only in other countries outside of the Middle East may infect residents of Israel who have not necessarily traveled overseas. Furthermore, this study reveals again that the epidemiology of the spotted fever group rickettsiae may not only involve Rickettsia conorii but may include other rickettsiae. PMID:24615133

Waner, Trevor; Keysary, Avi; Eremeeva, Marina E; Din, Adi Beth; Mumcuoglu, Kosta Y; King, Roni; Atiya-Nasagi, Yafit

2014-05-01

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Environmental infestation and rickettsial infection in ticks in an area endemic for Brazilian spotted fever / Infestacao ambiental e infeccao por rickettsias em carrapatos de area endemica para Febre Maculosa Brasileira  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese A Febre Maculosa Brasileira (FMB) é uma antropozoonose endêmica no município de Americana/SP, causada pela bactéria Rickettsia rickettsii e transmitida pelo carrapato Amblyomma cajennense. Este estudo avaliou a fauna de carrapatos e a infecção por riquétsias em carrapatos de vida livre capturados me [...] nsalmente com armadilhas de CO2, em áreas de risco para FMB de Americana, de julho de 2009 a junho de 2010. Duas espécies foram capturadas, A. cajennense (6.122 larvas; 4.265 ninfas; 2.355 adultos) e Amblyomma dubitatum (7.814 larvas; 3.364 ninfas; 1.193 adultos). Os estágios imaturos de A. cajennense e A. dubitatum apresentaram uma distribuição anual semelhante, com larvas de ambas as espécies sendo coletadas em maior número no período de abril a julho e ninfas de junho a outubro. Maior número de adultos de A. cajennense foi coletado de outubro a dezembro, enquanto que os adultos de A. dubitatum foram coletados em número relativamente semelhante durante todo o ano. A infecção por Rickettsia foi avaliada pela PCR em 1157 carrapatos A. cajennense e 1040 A. dubitatum, com apenas 41 (3,9%) A. dubitatum infectados com Rickettsia bellii. Este estudo demonstrou que as áreas de risco para FMB de Americana são caracterizadas por elevadas infestações ambientais de A. cajennense e A. dubitatum. Abstract in english Brazilian spotted fever (BSF), caused by Rickettsia rickettsii, is endemic in the municipality of Americana, southeastern Brazil, where the disease is transmitted by the tick Amblyomma cajennense. This study evaluated the tick fauna and rickettsial infection in free-living ticks that were captured m [...] onthly using dry ice traps in areas endemic for BSF in Americana, from July 2009 to June 2010. Two tick species were captured: A. cajennense (6,122 larvae; 4,265 nymphs; 2,355 adults) and Amblyomma dubitatum (7,814 larvae; 3,364 nymphs; 1,193 adults). The immature stages of A. cajennense and A. dubitatum had similar distribution through the 12-month period, with larvae of both species collected in highest numbers between April and July, and nymphs between June and October. The highest numbers of A. cajennense adults were collected between October and December, whereas A. dubitatum adults were collected in relatively similar numbers throughout the 12-month period. Rickettsial infection was evaluated by means of PCR in 1,157 A. cajennense and 1,040 A. dubitatum ticks; only 41 (3.9%) A. dubitatum were found to be infected by Rickettsia bellii. The present study showed that the areas of Americana that are endemic for BSF are characterized by high environmental burdens of A. cajennense and A. dubitatum.

Jose, Brites-Neto; Fernanda Aparecida, Nieri-Bastos; Jardel, Brasil; Keila Maria Roncato, Duarte; Thiago Fernandes, Martins; Cecilia Jose, Verissimo; Amalia Regina Mar, Barbieri; Marcelo Bahia, Labruna.

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Inactivation of rickettsiae.  

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A reliable and complete inactivation is an indispensable premise for any concentration of rickettsiae or for the development of diagnostic strategies based on their antigens. This study deals with the testing of methods to inactivate rickettsiae. Rickettsia honei was used as a model organism. The inactivating potency of formalin, Qiagen® antiviral lysozyme (AVL) buffer, heating to 56 °C, and ?-propiolactone was analyzed in cell culture. The inactivation limits for rickettsiae were 0.1% formalin about 10 min, Qiagen AVL buffer about 5 min, 56 °C about 5 min, 0.125% ?-propiolactone about 1 h, and 0.0125% ?-propiolactone overnight. The interpretation was limited by cytotoxic effects of the inactivation procedures and by the culturally achievable rickettsial density in the cell culture supernatants that were used for the inactivation experiments. Reliable modes of inactivation were identified, allowing for the secure handling of rickettsial antigens for diagnostic purposes. PMID:24265937

Frickmann, Hagen; Dobler, Gerhard

2013-09-01

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Approaches to Subunit Vaccines against the Typhus Rickettsiae, Rickettsia typhi and Rickettsia prowazekii.  

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We attempted to identify and purify those antigen components from Rickettsia typhi and Rickettsia prowazekii that are protective in forms which are both highly immunogenic and nontoxic. Subunit vaccines have obvious appeal in terms of purity and standardi...

G. A. Dasch J. P. Burans M. E. Dobson F. M. Rollwagen J. Misiti

1984-01-01

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Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil / Soroprevalência de Rickettsia bellii e Rickettsia felis em cães, São José dos Pinhais, Paraná, Brasil  

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Full Text Available SciELO Brazil | Language: English Abstract in portuguese A febre maculosa brasileira (FMB) é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, [...] estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI) contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis. Dezesseis amostras (4,4%) reagiram para pelo menos uma espécie de Rickettsia. Dos animais positivos, dois cães (15,5%) apresentaram títulos sugestivos de exposição a R. bellii. Uma amostra apresentou reação homóloga frente à R. felis, um agente patogênico confirmado para seres humanos. Muito embora os resultados demonstrem uma baixa prevalência de Rickettsia spp. em cães, sugerindo um baixo risco de infecção humana, este estudo relatou pela primeira vez a evidência de exposição a R. bellii e R. felis em cães no Sul do Brasil. Abstract in english Brazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, S [...] outhern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA) against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4%) reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5%) showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.

Fortes, Fernanda Silva; Silveira, Iara; Moraes-Filho, Jonas; Leite, Ronaldo Viana; Bonacim, José Edivaldo; Biondo, Alexander Welker; Labruna, Marcelo Bahia; Molento, Marcelo Beltrão.

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Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil Soroprevalência de Rickettsia bellii e Rickettsia felis em cães, São José dos Pinhais, Paraná, Brasil  

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Full Text Available Brazilian spotted fever (BSF is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4% reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5% showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.A febre maculosa brasileira (FMB é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis. Dezesseis amostras (4,4% reagiram para pelo menos uma espécie de Rickettsia. Dos animais positivos, dois cães (15,5% apresentaram títulos sugestivos de exposição a R. bellii. Uma amostra apresentou reação homóloga frente à R. felis, um agente patogênico confirmado para seres humanos. Muito embora os resultados demonstrem uma baixa prevalência de Rickettsia spp. em cães, sugerindo um baixo risco de infecção humana, este estudo relatou pela primeira vez a evidência de exposição a R. bellii e R. felis em cães no Sul do Brasil.

Fernanda Silva Fortes

2010-12-01

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Species-specific monoclonal antibodies to Rickettsia japonica, a newly identified spotted fever group rickettsia.  

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A total of 192 hybridomas were developed from mice immunized with Rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. Of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathogenic species of spotted fever group rickettsiae, Rickettsia akari, Rickettsia australis, Rickettsia conorii, Rickettsia rickettsii, and Rickettsia sibrica. Seven of the species-sp...

Uchiyama, T.; Uchida, T.; Walker, D. H.

1990-01-01

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O estadiamento ganglionar na actualidade / Node staging today  

Scientific Electronic Library Online (English)

Full Text Available SciELO Portugal | Language: Portuguese Abstract in portuguese A necessidade de realizar Esvaziamento Ganglionar Axilar quando o Gânglio Sentinela está metastizado, em doentes com Cancro da Mama, está a ser questionada. A maior parte dos doentes não apresenta metástases adicionais em Gânglios Não Sentinela e, em muito casos, a decisão de realizar tratamentos ad [...] juvantes não necessita da informação adicional fornecida pelo estudo dos gânglios axilares. A publicação recente do ensaio randomizado ACOSOG Z0011veio relançar a discussão sobre a necessidade e a utilidade do Esvaziamento Ganglionar Axilar. Abstract in english The need to perform an Axillary Dissection when the Sentinel Node is metastasized, in Breast Cancer patients, is being questioned. Most part of the patients do not have any additional metastasis in the Non Sentinel Nodes and, in many cases, the decision to perform adjuvant treatments is not dependen [...] t on the axillary nodes analysis. The recent publication of the randomized trial ACOSOG Z0011 launched the discussion on the need and usefulness of the axillary lymph node dissection.

Fougo, José Luís; Osório, Fernando; Costa, Susy; Magalhães, André.

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Detection of a Rickettsia Closely Related to Rickettsia aeschlimannii, “Rickettsia heilongjiangensis,” Rickettsia sp. Strain RpA4, and Ehrlichia muris in Ticks Collected in Russia and Kazakhstan  

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Using PCR, we screened 411 ticks from four genera collected in Russia and Kazakhstan for the presence of rickettsiae and ehrlichiae. In Russia, we detected “Rickettsia heilongjiangensis,” Rickettsia sp. strain RpA4, and Ehrlichia muris. In Kazakhstan, we detected Rickettsia sp. strain RpA4 and a rickettsia closely related to Rickettsia aeschlimannii. These agents should be considered in a differential diagnosis of tick-borne infections in these areas.

Shpynov, Stanislav; Fournier, Pierre-edouard; Rudakov, Nikolay; Tankibaev, Marat; Tarasevich, Irina; Raoult, Didier

2004-01-01

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Novel Rickettsia in Ticks, Tasmania, Australia  

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A novel rickettsia was detected in Ixodes tasmani ticks collected from Tasmanian devils. A total of 55% were positive for the citrate synthase gene by quantitative PCR. According to current criteria for rickettsia speciation, this new rickettsia qualifies as Candidatus Rickettsia tasmanensis, named after the location of its detection.

Izzard, Leonard; Graves, Stephen; Cox, Erika; Fenwick, Stan; Unsworth, Nathan; Stenos, John

2009-01-01

 
 
 
 
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Febre maculosa: isolamento de Rickettsia em amostra de biópsia de pele Spotted fever: Rickettsia isolation in skin biopsy sample  

Directory of Open Access Journals (Sweden)

Full Text Available Presença de Rickettsia na pele de doente de Febre Maculosa foi evidenciada por inoculação intraperitoneal em cobaio. O diagnóstico sorológico por imunoflüorescência indireta revelou diferença de título de anticorpos específicos para Rickettsia rickettsii, de 4 vezes entre a 1º e a 3º amostra. Imunoglobulina M (IgM específica foi detectada nas amostras de sangue, evidência de infecção em atividade ou recente. Foi também detectada a presença de anticorpos específicos para R. rickettsii no soro dos cobaios inoculados.A 2 years old child living in an area of the State of São Paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. Rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. In sera tested by indirect immunofluorescence with Rickettsia rickettsii standard antigen, IgG specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; IgM specific antibody titer was 1:128 in the three samples. Also positive were sera obtained from the inoculated guinea pigs. In the last 20 years no other case of rickettsial spotted fever has been confirmed by isolation of the agent in Brasil. To our knowlwdge, there are no previous reports of isolation of Rickettsiae through inoculation of skin biopsy homogenates.

Heloisa Helana B. Melles

1992-02-01

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Vaciamiento ganglionar en carcinoma escamoso transglótico / Ganglionic drainage in scaly transglotic carcinoma  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: El manejo de los ganglios cervicales es fundamental en el tratamiento y pronóstico de los pacientes con carcinoma escamoso de laringe. Existe consenso en realizar vaciamiento ganglionar cervical en ausencia de adenopatías clínicas (NO) cuando el riesgo de adenopatías metastásicas ocult [...] as supera el 20%. El carcinoma laríngeo transglótico (CTG) se caracteriza por presentar una incidencia de metástasis cervicales ocultas de 30% a 40%. Objetivo: Evaluar los hallazgos histopatológicos en los ganglios cervicales de los pacientes portadores de CTG, analizando la incidencia de metástasis ocultas y su asociación con factores de riesgo. Material y métodos: Estudio retrospectivo mediante la revisión de las fichas clínicas de pacientes portadores de CTG, sin tratamiento previo, manejados quirúrgicamente en el Hospital San Juan de Dios entre los años 1994 y 2002. Resultados: Se evaluaron 20 pacientes, 4 (20%) se presentaron con adenopatías clínicas, realizándose en 2 casos vaciamiento radical y en los 2 restantes disección funcional. Los pacientes NO fueron 16 y se les efectuó un vaciamiento funcional bilateral. Se detectaron metástasis ocultas en 12,5% de los pacientes NO. Conclusión: Los carcinomas de ubicación transglótica NO no se beneficiarían de un vaciamiento ganglionar cervical Abstract in english Introduction: The handling of the cervical ganglion is basic in the treatment and prognosis of patients with scaly carcinoma of the larynx. There is consensus on doing ganglionic cervical drainage in the absence of clinical adenopathies (NO) when the risk of hidden metastasic adenopathies is over 20 [...] %. Laryngeal transglotic carcinoma (LTC) is characterized for presenting an incidence of hidden cervical metastasis of 30 to 40%. Objective: To evaluate histopathological findings in the LTC patients' cervical ganglions, analyzing the incidence of hidden metastasis and their association with risk factors. Material and methods: Retrospective study through revision of the clinical files ofLTC carrier patients, without previous treatment, surgically treated at the San Juan de Dios Hospital between years 1994 and 2002. Results: 20 patients were evaluated, 4 (20%) presented clinical adenopathies, undergoing in 2 cases radical drainage and in the other 2, functional dissection. The NO patients were 16 and a bilateral functional drainage was made. Hidden metastasis were detected in 12,5% on the NO patients. Conclusion: Carcinomas of NO transglotic location did not benefit from a cervical ganglionic drainage

Juan, Carlos Bravo Y; Mariela C, Torrente A; Loreto A, Nicklas D.

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Vaciamiento ganglionar en carcinoma escamoso transglótico Ganglionic drainage in scaly transglotic carcinoma  

Directory of Open Access Journals (Sweden)

Full Text Available Introducción: El manejo de los ganglios cervicales es fundamental en el tratamiento y pronóstico de los pacientes con carcinoma escamoso de laringe. Existe consenso en realizar vaciamiento ganglionar cervical en ausencia de adenopatías clínicas (NO cuando el riesgo de adenopatías metastásicas ocultas supera el 20%. El carcinoma laríngeo transglótico (CTG se caracteriza por presentar una incidencia de metástasis cervicales ocultas de 30% a 40%. Objetivo: Evaluar los hallazgos histopatológicos en los ganglios cervicales de los pacientes portadores de CTG, analizando la incidencia de metástasis ocultas y su asociación con factores de riesgo. Material y métodos: Estudio retrospectivo mediante la revisión de las fichas clínicas de pacientes portadores de CTG, sin tratamiento previo, manejados quirúrgicamente en el Hospital San Juan de Dios entre los años 1994 y 2002. Resultados: Se evaluaron 20 pacientes, 4 (20% se presentaron con adenopatías clínicas, realizándose en 2 casos vaciamiento radical y en los 2 restantes disección funcional. Los pacientes NO fueron 16 y se les efectuó un vaciamiento funcional bilateral. Se detectaron metástasis ocultas en 12,5% de los pacientes NO. Conclusión: Los carcinomas de ubicación transglótica NO no se beneficiarían de un vaciamiento ganglionar cervicalIntroduction: The handling of the cervical ganglion is basic in the treatment and prognosis of patients with scaly carcinoma of the larynx. There is consensus on doing ganglionic cervical drainage in the absence of clinical adenopathies (NO when the risk of hidden metastasic adenopathies is over 20%. Laryngeal transglotic carcinoma (LTC is characterized for presenting an incidence of hidden cervical metastasis of 30 to 40%. Objective: To evaluate histopathological findings in the LTC patients' cervical ganglions, analyzing the incidence of hidden metastasis and their association with risk factors. Material and methods: Retrospective study through revision of the clinical files ofLTC carrier patients, without previous treatment, surgically treated at the San Juan de Dios Hospital between years 1994 and 2002. Results: 20 patients were evaluated, 4 (20% presented clinical adenopathies, undergoing in 2 cases radical drainage and in the other 2, functional dissection. The NO patients were 16 and a bilateral functional drainage was made. Hidden metastasis were detected in 12,5% on the NO patients. Conclusion: Carcinomas of NO transglotic location did not benefit from a cervical ganglionic drainage

Juan Carlos Bravo Y

2006-08-01

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The emerging diversity of Rickettsia  

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The best-known members of the bacterial genus Rickettsia are associates of blood-feeding arthropods that are pathogenic when transmitted to vertebrates. These species include the agents of acute human disease such as typhus and Rocky Mountain spotted fever. However, many other Rickettsia have been uncovered in recent surveys of bacteria associated with arthropods and other invertebrates; the hosts of these bacteria have no relationship with vertebrates. It is therefore perhaps more appropriat...

Perlman, Steve J.; Hunter, Martha S.; Zchori-fein, Einat

2006-01-01

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Rickettsia parkeri Rickettsiosis, Argentina.  

Science.gov (United States)

Rickettsia parkeri, a recently identified cause of spotted fever rickettsiosis in the United States, has been found in Amblyomma triste ticks in several countries of South America, including Argentina, where it is believed to cause disease in humans. We describe the clinical and epidemiologic characteristics of 2 patients in Argentina with confirmed R. parkeri infection and 7 additional patients with suspected R. parkeri rickettsiosis identified at 1 hospital during 2004-2009. The frequency and character of clinical signs and symptoms among these 9 patients closely resembled those described for patients in the United States (presence of an inoculation eschar, maculopapular rash often associated with pustules or vesicles, infrequent gastrointestinal manifestations, and relatively benign clinical course). Many R. parkeri infections in South America are likely to be misdiagnosed as other infectious diseases, including Rocky Mountain spotted fever, dengue, or leptospirosis. PMID:21762568

Romer, Yamila; Seijo, Alfredo C; Crudo, Favio; Nicholson, William L; Varela-Stokes, Andrea; Lash, R Ryan; Paddock, Christopher D

2011-07-01

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Genome Sequence of “Rickettsia sibirica subsp. mongolitimonae”  

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Rickettsia sibirica subsp. mongolitimonae” is the agent of lymphangitis-associated rickettsiosis, an emerging human disease that has been diagnosed in Europe and Africa. The present study reports the draft genome of Rickettsia sibirica subsp. mongolitimonae strain HA-91.

Sentausa, Erwin; El Karkouri, Khalid; Robert, Catherine; Raoult, Didier; Fournier, Pierre-edouard

2012-01-01

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Susceptibility of Ticks of the Superfamily Ixodoidea to Rickettsia Prowazeki.  

Science.gov (United States)

Susceptibility of ticks to Rickettsia prowazekii was tested experimentally. For infection of ticks they were either placed on infected guinea pigs or injected with rickettsia. Ticks H. anatolicum, D. pictus, A canestrinii picked up rickettsia during feedi...

I. M. Grokhovskaya V. F. Ignatovich V. E. Sidorov

1968-01-01

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21 CFR 866.3500 - Rickettsia serological reagents.  

Science.gov (United States)

...2009-04-01 2009-04-01 false Rickettsia serological reagents. 866.3500...Serological Reagents § 866.3500 Rickettsia serological reagents. (a) Identification. Rickettsia serological reagents are devices...

2009-04-01

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Plaque Assay for Rickettsia Rickettsii.  

Science.gov (United States)

A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special noble agar overlay. Plaques were counted in 6 days and resultant tite...

E. H. Weinberg J. R. Stakebake P. J . Gerone

1969-01-01

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Genomic Comparison of Rickettsia honei Strain RBT and Other Rickettsia Species  

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Rickettsia honei strain RBT was isolated from a febrile patient on Flinders Island, Australia, in 1991 and has been demonstrated to be the agent of Flinders Island spotted fever, a disease transmitted to humans by ticks. The comparison of this 1.27-Mb genome with other Rickettsia genomes provides additional insight into the mechanisms of evolution in Rickettsia species.

Xin, Dong; El Karkouri, Khalid; Robert, Catherine; Raoult, Didier; Fournier, Pierre-edouard

2012-01-01

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Factores predictivos de metástasis Ganglionares axilares, en Cáncer de mama menor de 2 centímetros  

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OBJETIVOS: Demostrar que existen factores clínicopatológicos para predecir metástasis ganglionares axilares en tumores de mama de más o menos 20 mm, de diámetro y también que la disección axilar es un procedimiento innecesario en la mayoría de estas pacientes, que puede omitirse con seguridad en aquellas pacientes con factores pronósticos favorables. MÉTODOS: Se realiza un estudio retrospectivo, revisándose los reportes macroscópicos, microscópicos, e inmunohistoquímica en los b...

2007-01-01

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El Cociente ganglionar como predictor de supervivencia en los carcinomas escamosos de cabeza y cuello  

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El cociente ganglionar o nodal ratio, definido como el cociente entre el número de adenopatías positivas dividido entre el número total de ganglios disecados, se ha comprobado como un factor pronóstico eficaz en diferentes tumores malignos. Los datos existentes en relación al papel del nodal ratio en la evaluación de los pacientes con carcinomas de cabeza y cuello (CEECC) son escasos y se concentran en una única localización: la cavidad oral. El objetivo principal del presente estudio...

2012-01-01

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El cociente ganglionar como predictor de supervivencia en los carcinomas escamosos de cabeza y cuello  

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El cociente ganglionar o nodal ratio, definido como el cociente entre el número de adenopatías positivas dividido entre el número total de ganglios disecados, se ha comprobado como un factor pronóstico eficaz en diferentes tumores malignos. Los datos existentes en relación al papel del nodal ratio en la evaluación de los pacientes con carcinomas de cabeza y cuello (CEECC) son escasos y se concentran en una única localización: la cavidad oral.

2012-01-01

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Rickettsia aeschlimannii in Hyalomma marginatum Ticks, Germany  

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To the Editor: Rickettsia spp. of the spotted fever group cause worldwide emerging human infections known as tick-borne rickettsioses (1). Data on the occurrence and prevalence of Rickettsia in Germany are still limited (2). Six Rickettsia species have been reported to date (2). R. helvetica, R. felis, R. massiliae, and R. monacensis were detected with a relatively low prevalence in Ixodes ricinus ticks collected in southern Germany (2); R. raoultii was identified with high prevalence in the ...

Rumer, Leonid; Graser, Elmara; Hillebrand, Timo; Talaska, Thomas; Dautel, Hans; Mediannikov, Oleg; Roy-chowdhury, Panchali; Sheshukova, Olga; Donoso Mantke, Oliver; Niedrig, Matthias

2011-01-01

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Permeability of Rickettsia prowazekii to NAD.  

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Rickettsia prowazekii accumulated radioactivity from [adenine-2,8-3H]NAD but not from [nicotinamide-4-3H]NAD, which demonstrated that NAD was not taken up intact. Extracellular NAD was hydrolyzed by rickettsiae with the products of hydrolysis, nicotinamide mononucleotide and AMP, appearing in the incubation medium in a time- and temperature-dependent manner. The particulate (membrane) fraction contained 90% of this NAD pyrophosphatase activity. Rickettsiae which had accumulated radiolabel aft...

Atkinson, W. H.; Winkler, H. H.

1989-01-01

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Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia  

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Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp., Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la...

2008-01-01

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Isolamento de Rickettsia em cultura de células vero / Isolation of Rickettsia in vero cell culture  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Embora o diagnóstico da febre maculosa baseie-se em sinais e sintomas característicos, o mesmo requer confirmação laboratorial, pois existem alguns diagnósticos diferenciais possíveis como meningococcemia, leptospirose, infecção por enterovírus e febre tifóide. A confirmação laboratorial pode ser fe [...] ita através da pesquisa de anticorpos específicos, possível somente alguns dias após o aparecimento da doença, através do isolamento do agente em amostras de sangue e/ou biópsia de pele, e ainda, de amostras de carrapatos coletados do paciente ou de animais reservatório. O isolamento a partir de sangue ou biópsia de pele resulta em diagnóstico precoce da doença, pois na fase de rickettsemia ainda não há anticorpos detectáveis no sangue. Assim, com o objetivo de facilitar o diagnóstico precoce da febre maculosa, estabelecemos um método de isolamento de rickettsia em cultura de células vero. Para a padronização foi inoculada amostra padrão de Rickettsia rickettsii, cepa Sheyla Smith, cedida pelo CDC. A identificação foi feita através da reação de imunofluorescência indireta. A presença de microrganismos verdes fluorescentes visualizados no interior do citoplasma das células caracterizou o crescimento do agente. Posteriormente, a metodologia foi confirmada pelo isolamento do agente da febre maculosa em amostras de biópsia de pele de paciente proveniente de área endêmica no Estado de São Paulo, bem como, de amostras de carrapato do gênero Amblyomma, considerado o reservatório e transmissor da doença no Brasil. Abstract in english The diagnosis of spotted fever is based on characteristic signs and symptoms but requires laboratorial confirmation because of the possible differencial diagnosis from other diseases like leptospirosis, enterovirosis, meningococcemia and tiphoid fever. Laboratorial confirmation may be done by detect [...] ion of specific antibodies which is possible only 5-10 days after the onset of the symptoms or by the isolation of Rickettsiae from blood and/or skin biopsy and from ticks collected in the patient or in the animal reservoir. The isolation of Rickettsiae from blood or skin biopsy results in an early diagnosis of spotted fever since in the rickettsiemic phase of the disease there is no detectable level of antibodies in the serum. With the purpose of facilitating the diagnosis of Spotted Fever we have standardized the isolation of Rickettsiae in cell culture by a method that is less time consuming and that reduces the biological risks than isolation in guinea pigs. vero cell cultures were inoculated with the Sheyla Smith strain of Rickettsia rickettsii provided by CDC (Atlanta-USA). The identification was performed by indirect immunofluorescence technique. The presence of green fluorescent organisms characterized the growth of the agent. Ulterior confirmation of the methodology was done by isolation of the spotted fever agent from skin biopsy of a patient from an endemic area and from Amblyomma ticks that are the reservoir and vector of the Brazilian spotted fever.

Melles, Heloisa Helena Barbosa; Colombo, Silvia; Lemos, Elba Regina Sampaio de.

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Rickettsia felis: un patógeno emergente en Latinoamérica / Rickettsia felis: an emerging pathogen in Latin America  

Scientific Electronic Library Online (English)

Full Text Available SciELO Costa Rica | Language: Spanish Abstract in spanish El primer caso humano infectado con Rickettsia felis fue descrito en un paciente diagnosticado con tifus murino en Estados Unidos en 1994. Los reportes acerca de la presencia de R. felis se han incrementado en los últimos años y ya se ha reportado en la mayoría de los países de América Latina. Los s [...] íntomas y signos de la enfermedad causada por R. felis son inespecíficos y es importante que cada país lo incluya en el diagnóstico diferencial con respecto a otras enfermedades que tienen las mismas manifestaciones clínicas. Abstract in english The first human case of Rickettsia felis was described in 1994, in a patient diagnosed with murine typhus in the United States. Reports about the presence of R. felis have increased in the last years, and it has been reported in most countries of Latin America. Signs and symptoms of disease caused b [...] y R. felis are unspecific, and it is important for each country to include it in the differential diagnosis with other diseases that show the same clinical manifestations.

Zavala Castro, Jorge E.

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Evolution and diversity of Rickettsia bacteria  

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Full Text Available Abstract Background Rickettsia are intracellular symbionts of eukaryotes that are best known for infecting and causing serious diseases in humans and other mammals. All known vertebrate-associated Rickettsia are vectored by arthropods as part of their life-cycle, and many other Rickettsia are found exclusively in arthropods with no known secondary host. However, little is known about the biology of these latter strains. Here, we have identified 20 new strains of Rickettsia from arthropods, and constructed a multi-gene phylogeny of the entire genus which includes these new strains. Results We show that Rickettsia are primarily arthropod-associated bacteria, and identify several novel groups within the genus. Rickettsia do not co-speciate with their hosts but host shifts most often occur between related arthropods. Rickettsia have evolved adaptations including transmission through vertebrates and killing males in some arthropod hosts. We uncovered one case of horizontal gene transfer among Rickettsia, where a strain is a chimera from two distantly related groups, but multi-gene analysis indicates that different parts of the genome tend to share the same phylogeny. Conclusion Approximately 150 million years ago, Rickettsia split into two main clades, one of which primarily infects arthropods, and the other infects a diverse range of protists, other eukaryotes and arthropods. There was then a rapid radiation about 50 million years ago, which coincided with the evolution of life history adaptations in a few branches of the phylogeny. Even though Rickettsia are thought to be primarily transmitted vertically, host associations are short lived with frequent switching to new host lineages. Recombination throughout the genus is generally uncommon, although there is evidence of horizontal gene transfer. A better understanding of the evolution of Rickettsia will help in the future to elucidate the mechanisms of pathogenicity, transmission and virulence.

Stone Graham N

2009-02-01

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Rickettsia helvetica in Dermacentor reticulatus Ticks  

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We report on the molecular evidence that Dermacentor reticulatus ticks in Croatia are infected with Rickettsia helvetica (10%) or Rickettsia slovaca (2%) or co-infected with both species (1%). These findings expand the knowledge of the geographic distribution of R. helvetica and D. reticulatus ticks.

Dobec, Marinko; Golubic, Dragutin; Punda-polic, Volga; Kaeppeli, Franz; Sievers, Martin

2009-01-01

 
 
 
 
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Rickettsia parkeri in Amblyomma triste from Uruguay  

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Our goal was to detect whether spotted fever group Rickettsia are found in the suspected vector of rickettsioses, Amblyomma triste, in Uruguay. Rickettsia parkeri was detected in A. triste, which suggests that this species could be considered a pathogenic agent responsible for human rickettsioses in Uruguay.

Venzal, Jose? M.; Portillo, Ara?nzazu; Estrada-pen?a, Agusti?n; Castro, Oscar; Cabrera, Perla A.; Oteo, Jose? A.

2004-01-01

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Neuroprotección de las células ganglionares de la retina / Retinal ganglion cell neuroprotection in culture  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Objetivo: Estudiar la supervivencia de las células ganglionares de la retina (CGR) de cerdo en cultivo, analizando el posible efecto neuroprotector de las células de Müller de la retina (CMR) y del factor neurotrófico derivado del cerebro (BDNF). Métodos: Las retinas de cerdo adulto fueron disociada [...] s y cultivadas en diferentes condiciones: 1) sobre sustrato de laminina/poli-D-lisina en medio de cultivo químicamente definido; 2) sobre sustrato de laminina/poli-D-lisina en medio químicamente definido al que se añadió BDNF; 3) sobre monocapas de CMR en medio químicamente definido; 4) sobre sustrato de laminina/poli-D-lisina en medio condicionado procedente del sobrenadante de las CMR. Las CGR fueron identificadas mediante inmunocitoquímica, utilizando anticuerpos contra el neurofilamento de 68 kDa, y observadas con un microscopio de fluorescencia. Se analizó la supervivencia para cada condición de cultivo y se clasificaron las CGR en función de su tamaño y del número y longitud de las neuritas. Resultados: La supervivencia de las CGR aumentó cuando las células fueron cultivadas sobre monocapas confluentes de CMR o en medio condicionado. Estas condiciones produjeron un incremento en el área media de las células y un aumento en el número de neuritas emitidas por cada célula, así como en la longitud de las neuritas. Cuando el medio de cultivo se suplementó con BDNF no se obtuvo ningún efecto sobre la supervivencia de las CGR aunque aumentó el tamaño, y el número y longitud de sus neuritas. Conclusión: Nuestro trabajo demuestra que algún/os factor/es secretados por las células de Müller tienen un efecto neuroprotector sobre las CGR in vitro. El BDNF produce también un incremento en el área media de las células y favorece la formación de neuritas, sin embargo no aumenta la supervivencia. Abstract in english Purpose: To study the pig retinal ganglion cell (RGC) survival in culture, analysing the possible neuroprotective effect of retinal Müller glia (RMG) and brain-derived neurotrophic factor (BDNF). Methods: Adult pig retina were dissociated and cultured under different conditions: 1) on laminin/poly-D [...] -lysine-coated coverslips in chemically defined medium (CDM); 2) on laminin/poly-D-lysine-coated coverslips in CMD supplemented with BDNF; 3) on confluent monolayer cultures of RMG in CDM; 4) on laminin/poly-D-lysine substrate in conditioned medium obtained from RMG. RGCs were identified by immunocytochemistry using antibody against 68 kDa neurofilament and observed under an fluorescent microscope. RGCs were classified on the basis of the size, number and length of neurites, and their survival was assayed for each treatment. Results: Confluent RMG substrates and RMG conditioned medium significantly increased the survival of cultured pig RGC. Moreover these two conditions increased the mean area of RGCs and enhanced neurite growth and elongation. Addition of BDNF to culture medium did not modify survival but increased RGC size, neurite number and neurite length. Conclusions: These findings demonstrate that factor(s) secreted by RMG exert beneficial effects on adult RGC survival and neurite regeneration in vitro, and might constitute important agent(s) for RGC neuroprotection. BDNF also increases the mean area of RGCs and enhances neurite growth but it does not increase the survival of RGCs.

M, García; J, Ruiz Ederra; E, Hernández Barbáchano; JA, Urcola; J, Bilbao; J, Araiz; JA, Durán; E, Vecino.

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Rickettsia species infecting Amblyomma ticks from an area endemic for Brazilian spotted fever in Brazil Rickettsia infectando carrapatos Amblyomma de uma área endêmica para febre maculosa Brasileira no Brasil  

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Full Text Available This study reports rickettsial infection in Amblyomma cajennense and Amblyomma dubitatum ticks collected in an area of the state of Minas Gerais, Brazil, where Brazilian spotted fever is considered endemic. For this purpose, 400 adults of A. cajenennse and 200 adults of A. dubitatum, plus 2,000 larvae and 2,000 nymphs of Amblyomma spp. were collected from horses and from the vegetation. The ticks were tested for rickettsial infection through polymerase chain reaction (PCR protocols targeting portions of three rickettsial genes (gltA, ompA, and ompB. Only two free-living A. cajennense adult ticks, and four pools of free-living Amblyomma spp. nymphs were shown to contain rickettsial DNA. PCR products from the two A. cajennense adult ticks were shown to be identical to corresponding sequences of the Rickettsia rickettsii strain Sheila Smith. DNA sequences of gltA-PCR products of the four nymph pools of Amblyomma spp. revealed a new genotype, which was shown to be closest (99.4% to the corresponding sequence of Rickettsia tamurae. Our findings of two R. rickettsii-infected A. cajennense ticks corroborate the endemic status of the study area, where human cases of BSF were reported recently. In addition, we report for the first time a new Rickettsia genotype in Brazil.Este trabalho relata infecção por Rickettsia em carrapatos Amblyomma cajennense e Amblyomma dubitatum, colhidos numa área do Estado de Minas Gerais, onde a febre maculosa brasileira (FMB é considerada endêmica. Para esse estudo, 400 adultos de A. cajennense, 200 adultos de A. dubitatum, 2.000 larvas e 2.000 ninfas de Amblyomma spp. foram colhidas de equinos e da vegetação. Os carrapatos foram testados para infecção por rickettsia através de reação em cadeia pela polimerase (PCR direcionada a fragmentos de três genes de rickettsia (gltA, ompA, e ompB. Apenas 2 A. cajennense adultos de vida livre, e 4 grupos de ninfas de Amblyomma spp. continham DNA de rickettsia. Os produtos de PCR dos dois adultos de A. cajennense foram idênticos às sequências correspondentes de Rickettsia rickettsii cepa Sheila Smith. Sequências de DNA dos produtos provenientes dos quatro grupos de ninfas de Amblyomma spp. revelaram um novo genótipo, próximo (99,4% à sequência correspondente de Rickettsia tamurae. Neste trabalho foram achados 2 carrapatos A. cajennense infectados por R. rickettsii que corroboram o caráter endêmico da área de estudo, em que casos de FMB ocorreram recentemente. Em adição, foi reportado, pela primeira vez, um novo genótipo de Rickettsia no Brasil.

Elizângela Guedes

2011-12-01

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Rickettsia species infecting Amblyomma ticks from an area endemic for Brazilian spotted fever in Brazil / Rickettsia infectando carrapatos Amblyomma de uma área endêmica para febre maculosa Brasileira no Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Este trabalho relata infecção por Rickettsia em carrapatos Amblyomma cajennense e Amblyomma dubitatum, colhidos numa área do Estado de Minas Gerais, onde a febre maculosa brasileira (FMB) é considerada endêmica. Para esse estudo, 400 adultos de A. cajennense, 200 adultos de A. dubitatum, 2.000 larva [...] s e 2.000 ninfas de Amblyomma spp. foram colhidas de equinos e da vegetação. Os carrapatos foram testados para infecção por rickettsia através de reação em cadeia pela polimerase (PCR) direcionada a fragmentos de três genes de rickettsia (gltA, ompA, e ompB). Apenas 2 A. cajennense adultos de vida livre, e 4 grupos de ninfas de Amblyomma spp. continham DNA de rickettsia. Os produtos de PCR dos dois adultos de A. cajennense foram idênticos às sequências correspondentes de Rickettsia rickettsii cepa Sheila Smith. Sequências de DNA dos produtos provenientes dos quatro grupos de ninfas de Amblyomma spp. revelaram um novo genótipo, próximo (99,4%) à sequência correspondente de Rickettsia tamurae. Neste trabalho foram achados 2 carrapatos A. cajennense infectados por R. rickettsii que corroboram o caráter endêmico da área de estudo, em que casos de FMB ocorreram recentemente. Em adição, foi reportado, pela primeira vez, um novo genótipo de Rickettsia no Brasil. Abstract in english This study reports rickettsial infection in Amblyomma cajennense and Amblyomma dubitatum ticks collected in an area of the state of Minas Gerais, Brazil, where Brazilian spotted fever is considered endemic. For this purpose, 400 adults of A. cajenennse and 200 adults of A. dubitatum, plus 2,000 larv [...] ae and 2,000 nymphs of Amblyomma spp. were collected from horses and from the vegetation. The ticks were tested for rickettsial infection through polymerase chain reaction (PCR) protocols targeting portions of three rickettsial genes (gltA, ompA, and ompB). Only two free-living A. cajennense adult ticks, and four pools of free-living Amblyomma spp. nymphs were shown to contain rickettsial DNA. PCR products from the two A. cajennense adult ticks were shown to be identical to corresponding sequences of the Rickettsia rickettsii strain Sheila Smith. DNA sequences of gltA-PCR products of the four nymph pools of Amblyomma spp. revealed a new genotype, which was shown to be closest (99.4%) to the corresponding sequence of Rickettsia tamurae. Our findings of two R. rickettsii-infected A. cajennense ticks corroborate the endemic status of the study area, where human cases of BSF were reported recently. In addition, we report for the first time a new Rickettsia genotype in Brazil.

Guedes, Elizângela; Leite, Romário Cerqueira; Pacheco, Richard Campos; Silveira, Iara; Labruna, Marcelo Bahia.

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PCR MÚLTIPLE ANIDADA PARA DETECCIÓN DE FITOPLASMAS Y RICKETTSIA ASOCIADOS CON LOS SÍNTOMAS DEL COGOLLO ARREPOLLADO (BTS) EN PAPAYO / NESTED PCR MULTIPLEX FOR THE DETECTION OF THE PHYTOPLASMAS AND RICKETTSIA ASSOCIATED WITH BUNCHY TOP SYMPTOM (BTS) IN PAPAYO  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Recientemente, los grupos fitoplasmas 16SrI «Candidatus Phytoplasma asteris»,16SrII «Candidatus Phytoplasma aurantifolia» y rickettsia se asociaron a síntomas del cogollo arrepollado (BTS) del papayo en Cuba. El ADN de muestras de plantas de papayo positivas a fitoplasmas y rickettsia se empleó para [...] optimizar y evaluar un ensayo de PCR múltiple anidada. Se usaron los iniciadores de PCR genéricos para fitoplamas R16mF2/R16mR1en la primera amplificación y en la segunda amplificación una mezcla PBTF1/PBTR1 (específicos para rickettsia) y R16F2n/BPVNr/p86r (específicos para los grupos de fitoplasmas 16SrI y 16SrII). El ensayo de diagnóstico por PCR múltiple anidado permitió la detección simultánea de fitoplasmas y rickettsia en muestras de plantas de papayo de condiciones de campo. Abstract in english Recently, the phytoplasmas groups 16SrI `Candidatus Phytoplasma aurantifolia' and 16SrII group `Candidatus Phytoplasma asteris' and rickettsia were associated with Bunchy Top Symptoms (BTS) of papayo in Cuba. The ADN samples from papaya plants positive to phytoplasma and rickettsia were used for eva [...] luate and optimize nPCR multiplex. Generic primers for amplification of phytoplasma R16mF2/R16mR1 were used in the first reaction. A mixture composed of PBTF1/PBTR1and R16F2n/BPVNr/p86r for rickettsia and phytoplasma (16SrI and 16SrII groups) amplifications were used in the second reaction. The diagnostic assay by the nPCR multiplex permitted the simultaneous detection of phytoplasmas and rickettsias in samples of papaya plants from the field.

Acosta, K; Martínez, Y; Zamora, L; Fernández, A; Santos-Cervantes, M.E; Leyva-López, N.E.

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Antigenic diversity of Rickettsia conorii.  

Science.gov (United States)

Analysis of seven strains designated as Rickettsia conorii for reactivity with a panel of 12 monoclonal antibodies to surface-protein epitopes of spotted fever group rickettsiae and by Western immunoblotting with standard serotyping sera revealed remarkable antigenic diversity. Rickettsial strains from France, Morocco, Ethiopia, Kenya, South Africa, India, and the USSR differed from one another in reactivity with at least one and as many as five monoclonal antibodies. Simko and Indian strains were similar to one another and differed substantially from other R. conorii strains. All seven strains reacted with three R. conorii-specific monoclonal antibodies. Western immunoblotting demonstrated a major 120-kD protein and a major 135-kD protein in all strains. The principal differences were the presence of a major undenatured 130-kD protein in all strains except Indian and Simko, which had an analogous protein of 124 kD. Immunodominant antigenically related, heat-denatured protein bands of 170 kD (Malish 7 strain), 175 kD (Manuel strain), and 190 kD (Kenya tick typhus, Indian, and Simko strains) were not detected in the M-1 and Moroccan strains. This antigenic diversity is greater than that previously reported for other spotted fever group rickettsial species, suggesting that R. conorrii is an older species than R. rickettsii with a longer period of time for evolutionary divergence. PMID:1379008

Walker, D H; Liu, Q H; Yu, X J; Li, H; Taylor, C; Feng, H M

1992-07-01

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Large lymph node size harvested as prognostic factor in gastric cancer? ¿Es el diámetro ganglionar mayor un factor pronóstico en cáncer gástrico?  

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Full Text Available Objective: knowledge regarding prognostic factors in gastric cancer is essential to decide on single patient management. We aim to establish the value of large lymph node size in order to improve perioperative approach. Material and methods: charts of one hundred and twenty-eight consecutive patients undergoing gastrectomy for resectable gastric cancer were reviewed between January 1996 and December 2005. Patients were split in two groups according to large lymph node size harvested, group I, lymph node size ? 10 mm and group II, lymph node size > 10 mm. Overall five-year survival related to cancer were analyzed as a main endpoint. Prognostic factors as TNM classification and degree of differentiation have been considered. Results: there were no differences regarding age and gender (67.4 vs. 64; p = 0.34 and 66,1 vs. 68,1%; p = 0.27, respectively. Nevertheless, a significant difference has been found according to T1-T2 of TNM stage (78.1 vs. 39.1% p = Objetivo: valorar el interés del diámetro del ganglio mayor extirpado como factor pronóstico en los pacientes intervenidos por cáncer gástrico, para determinar si su detección puede ser un factor de interés en el periodo preoperatorio, para indicar tratamiento neoadyuvante. Material y métodos: se analiza un registro de 128 casos consecutivos de pacientes afectos de adenocarcinoma gástrico resecable, durante un periodo de 10 años en los que en el estudio anatomopatológico se determinó el diámetro del ganglio mayor aislado. Se estudia la relación del mismo con factores pronósticos universalmente aceptados, el grado de penetración, la presencia y extensión de metástasis ganglionares y el estadio TNM, y con la supervivencia a 5 años, estudiándose dos grupos, el grupo I compuesto por aquellos enfermos con diámetro menor o igual a 10 mm, y el grupo II con diámetros superiores a 10 mm. Resultados: no se han detectado diferencias estadísticas respecto a edad y sexo (67,4 vs. 64; p = 0,34 y 66,1 vs. 68,1%; p = 0,27, respectivamente. Existen diferencias significativas entre ambos grupos en el grado de penetración tumoral, T1-T2, (78,1% por 39,1%, p < 0,001, en el porcentaje de pacientes sin metástasis ganglionares (62,7 vs. 30,5%; p < 0,001, así como en el porcentaje de estadios precoces (Ia y Ib, 57,6% por 17,4, p < 0,001. La supervivencia global acumulada a los 60 meses fue significativa entre ambos grupos (p log-rank = 0,0003, aunque sin alcanzar significación estadística en los pacientes N+ (p < 0,006. Conclusiones: la relación del diámetro ganglionar mayor puede ser un factor pronóstico útil y junto con otros factores pronósticos facilitaría la valoración de quimioterapia neoadyuvante. Su detección mediante exploraciones complementarias adquiriría por consiguiente un mayor interés.

F. Espín

2010-03-01

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Novel Clade of Rickettsia spp. from Leeches  

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Intracellular rickettsia-like structures were found in the tissues of a glossiphoniid leech, Torix tagoi, by transmission electron microscopy. Diagnostic PCR analysis using specific primers suggested that of the nine glossiphoniid species examined, two species, T. tagoi and Hemicrepsis marginata, harbored bacteria of the genus Rickettsia. A 1.5-kb eubacterial 16S rRNA gene segment obtained from each of these species was amplified by PCR, cloned, and sequenced. Phylogenetic analysis of the 16S...

Kikuchi, Yoshitomo; Sameshima, Shinya; Kitade, Osamu; Kojima, Junichi; Fukatsu, Takema

2002-01-01

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Detection and identification of Rickettsia helvetica and Rickettsia sp. IRS3/IRS4 in Ixodes ricinus ticks found on humans in Spain  

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New Rickettsia spp. are continuously being isolated from ticks around the world, but in most cases their pathogenicity remains to be determined. Some rickettsiae first thought to be nonpathogenic have later been associated with human disease, such as Rickettsia slovaca, Rickettsia helvetica, Rickettsia aeschlimannii and, more recently, the Spanish strain Bar29 (Rickettsia massiliae genogroup), which seems to play a role in the pathogenesis of Mediterranean spotted fever. There are many ...

Ferna?ndez Soto, Pedro; Pe?rez Sa?nchez, Ricardo; Encinas Grandes, Antonio; A?lamo Sanz, Rufino

2004-01-01

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Assessment of Real-Time PCR Assay for Detection of Rickettsia spp. and Rickettsia rickettsii in Banked Clinical Samples  

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Two novel real-time PCR assays were developed for the detection of Rickettsia spp. One assay detects all tested Rickettsia spp.; the other is specific for Rickettsia rickettsii. Evaluation using DNA from human blood and tissue samples showed both assays to be more sensitive than nested PCR assays currently in use at the CDC.

Kato, Cecilia Y.; Chung, Ida H.; Robinson, Lauren K.; Austin, Amy L.; Dasch, Gregory A.; Massung, Robert F.

2013-01-01

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Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia  

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Full Text Available Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp., Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la técnica de ELISA indirecta. Para la determinación de Rickettsia sp. y Ehrlichia sp. se uso la técnica de inmunofluorescencia indirecta. Resultados. La población evaluada estaba compuesta por 27 (30% ordeñadores, 21 (23% jornaleros, 18 (20% profesionales del campo y 24 (27% que realizaban otras actividades. Ventidós (24% muestras resultaron positivas en alguna de las pruebas. De éstas, 12 (13,3% fueron positivas para Leptospira sp., 7 (7,8% para Rickettsia sp. y 3 (3,3% ara Ehrlichia sp. Conclusión. Este fue el primer estudio que se llevó a cabo en el departamento de Sucre y permitió demostrar que existe una prevalencia importante de Leptospira p.,Rickettsia sp. y Ehrlichia sp.. Los factores de riesgo ocupacional fueron factores determinantes en la seropositividad.Objective. To determine the seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in agricultural workers of Sucre. Methods. A descriptive prospective cross-sectional study was conducted in ninety rural workers of Sucre. Presence of serum antibodies anti-IgM specific anti-Leptospira by indirect ELISA was established. For the determination of Rickettsia and Ehrlichia indirect inmunoflorescence was used. Results.The population was composed by 27 (30% milkers, 21 (23% day workers, 18 farm professionals (20% and 24 (26% workers in others activities. A total of 22 (24% samples were positive to some test. Twelve (13.3% were positive to Leptospira sp., seven (7.8% to Rickettsia sp. and three (3.3% o Ehrlichia sp.. Conclusions. This is the first study carried out in Sucre; there is an important prevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp..The occupational risk factors were decisive in the seropositivity.

Rodrigo Ríos

2008-06-01

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Rickettsia typhi and Rickettsia felis in Xenopsylla cheopis and Leptopsylla segnis Parasitizing Rats in Cyprus  

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Fleas collected from rats during a three-year period (2000–2003) in 51 areas of all provinces of Cyprus were tested by molecular analysis to characterize the prevalence and identity of fleaborne rickettsiae. Rickettsia typhi, the causative agent of murine typhus, was detected in Xenopsylla cheopis (4%) and in Leptopsylla segnis (6.6%). Rickettsia felis was detected in X. cheopis (1%). This is the first report of R. typhi in X. cheopis and L. segnis from rats, in Cyprus, and the first report...

Christou, Christos; Psaroulaki, Anna; Antoniou, Maria; Toumazos, Pavlos; Ioannou, Ioannis; Mazeris, Apostolos; Chochlakis, Dimosthenis; Tselentis, Yannis

2010-01-01

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Isolamento de Rickettsia em cultura de células vero  

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Full Text Available Embora o diagnóstico da febre maculosa baseie-se em sinais e sintomas característicos, o mesmo requer confirmação laboratorial, pois existem alguns diagnósticos diferenciais possíveis como meningococcemia, leptospirose, infecção por enterovírus e febre tifóide. A confirmação laboratorial pode ser feita através da pesquisa de anticorpos específicos, possível somente alguns dias após o aparecimento da doença, através do isolamento do agente em amostras de sangue e/ou biópsia de pele, e ainda, de amostras de carrapatos coletados do paciente ou de animais reservatório. O isolamento a partir de sangue ou biópsia de pele resulta em diagnóstico precoce da doença, pois na fase de rickettsemia ainda não há anticorpos detectáveis no sangue. Assim, com o objetivo de facilitar o diagnóstico precoce da febre maculosa, estabelecemos um método de isolamento de rickettsia em cultura de células vero. Para a padronização foi inoculada amostra padrão de Rickettsia rickettsii, cepa Sheyla Smith, cedida pelo CDC. A identificação foi feita através da reação de imunofluorescência indireta. A presença de microrganismos verdes fluorescentes visualizados no interior do citoplasma das células caracterizou o crescimento do agente. Posteriormente, a metodologia foi confirmada pelo isolamento do agente da febre maculosa em amostras de biópsia de pele de paciente proveniente de área endêmica no Estado de São Paulo, bem como, de amostras de carrapato do gênero Amblyomma, considerado o reservatório e transmissor da doença no Brasil.

Melles Heloisa Helena Barbosa

1999-01-01

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Identificación de Rickettsia andeanae en dos regiones de Chile / Detection of Rickettsia andeanae in two regions of Chile  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: Candidatus 'Rickettsia andeanae', especie de reciente reconocimiento y rol patógeno no precisado, ha sido identificada en garrapatas del género Amblyomma en Perú, E.U.A. y AArgentina y recientemente en Chile, en un espécimen de Amblyomma triste de un sector rural de Arica. Objetivo: De [...] terminar presencia de agentes rickettsiales en garrapatas de perros domésticos de dos regiones de Chile. Métodos: Estudio transversal, descriptivo, en las regiones de Coquimbo y La Araucanía, con muestreo domiciliario en las ciudades de Coquimbo y AAngol y localidades rurales cercanas, en primavera-verano 2011-2012. Se examinó un perro por vivienda, recolectando garrapatas si estaban presentes; luego de su identificación taxonómica fueron sometidas a amplificación y secuenciación de los genes gltA y ompA. Resultados: Se examinaron 462 perros, 255 tenían garrapatas (55%). En las ciudades se encontró exclusivamente Rhipicephalus sanguineus; en las localidades rurales además Amblyomma tigrinum. Las secuencias obtenidas en 12 especímenes de A. tigrinum correspondieron a Candidatus 'Rickettsia andeanae'. Conclusiones: Se documenta presencia de R. andeanae en dos regiones distantes de Chile, lo que se suma a su reciente hallazgo en el extremo norte del país. Este nuevo agente rickettsial se limita a garrapatas del género Amblyomma y a zonas rurales, lo que concuerda con lo documentado en otros países y con el hábitat de esta garrapata. Abstract in english Introduction: Candidatus 'Rickettsia andeanae' is recently recognized specie, with no attributed pathogenic role so far. It has been reported in Amblyomma ticks from Peru, USA and Argentina, and recently in Chile, in one specimen of Amblyomma triste from a rural locality in Arica. Objective: To dete [...] rmine the presence of rickettsial agents in ticks from domestic dogs from two Chilean regions. Methods: A transversal descriptive study in Coquimbo and La Araucanía regions, consisted in households sampling in the cities of Coquimbo and Aingol and nearby rural localities, in spring-summer 2011-2012. One dog per house was examined and ticks were collected when present; after taxonomic identification, gltA and ompA genes were amplified and sequenced. Results: 462 dogs were examined, 255 had ticks (55%). In the cities just Rhipicephalus sanguineus was found; in rural localities also Amblyomma tigrinum. Obtained sequences in 12 specimens from A. tigrinum corresponded to Candidatus 'Rickettsia andeanae'. Conclusions: We documented the presence of R. andeanae in two distant Chilean regions, which is added to its recently finding in the Northern extreme of the country. This new rickettsial agent limits to Amblyomma ticks and rural areas, as it was reported in other American countries and corresponds with this tick habitat.

Katia, Abarca; Javier, López; Gerardo, Acosta-Jamett; Constanza, Martinez-Valdebenito.

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Spotted fever group rickettsiae in ticks, Morocco.  

Science.gov (United States)

A total of 370 ticks, encompassing 7 species from 4 genera, were collected during 2002-2006 from domestic animals and vegetation in the Taza region of northeastern Morocco. Rickettsial DNA was identified in 101 ticks (27%) by sequencing PCR products of fragments of the citrate synthase and outer membrane protein genes of Rickettsia spp. Seven rickettsiae of the spotted fever group were identified, including 4 pathogens: R. aeschlimannii in Hyalomma marginatum marginatum, R. massiliae in Rhipicephalus sanguineus, R. slovaca in Dermacentor marginatus, and R. monacensis in Ixodes ricinus. Two suspected pathogens were also detected (R. raoultii in D. marginatus and R. helvetica in I. ricinus). An incompletely described Rickettsia sp. was detected in Haemaphysalis spp. ticks. PMID:18598627

Sarih, Mhammed; Socolovschi, Cristina; Boudebouch, Najma; Hassar, Mohammed; Raoult, Didier; Parola, Philippe

2008-07-01

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Rickettsia raoultii, the predominant Rickettsia found in Dermacentor silvarum ticks in China-Russia border areas.  

Science.gov (United States)

Since the year 2000, clinical patterns resembling tick-borne rickettsioses have been noticed in China-Russia border areas. Epidemiological data regarding species of the aetiological agent, tick vector prevalence and distribution as well as incidence of human cases in the areas are still sparse to date. In order to identify Rickettsia species occurring in the areas, we investigated Dermacentor silvarum collected in the selected areas. Rickettsia raoultii was the predominant Rickettsia found in D. silvarum evident with ompA, ompB, gltA and 17 kDa protein genes. The Rickettsia prevalence in D. silvarum appeared to be 32.25 % with no sex difference. The results extend the common knowledge about the geographic distribution of R. raoultii and its candidate vector tick species, which suggest an emerged potential threat of human health in the areas. PMID:24699771

Wen, Jing; Jiao, Dan; Wang, Jian-Hua; Yao, De-Hai; Liu, Zhi-Xiang; Zhao, Gang; Ju, Wen-Dong; Cheng, Cheng; Li, Yi-Jing; Sun, Yi

2014-08-01

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Factores predictivos de metástasis Ganglionares axilares, en Cáncer de mama menor de 2 centímetros  

Scientific Electronic Library Online (English)

Full Text Available SciELO Venezuela | Language: Spanish Abstract in spanish OBJETIVOS: Demostrar que existen factores clínicopatológicos para predecir metástasis ganglionares axilares en tumores de mama de más o menos 20 mm, de diámetro y también que la disección axilar es un procedimiento innecesario en la mayoría de estas pacientes, que puede omitirse con seguridad en aqu [...] ellas pacientes con factores pronósticos favorables. MÉTODOS: Se realiza un estudio retrospectivo, revisándose los reportes macroscópicos, microscópicos, e inmunohistoquímica en los bloques celulares de pacientes con carcinoma mamario de tamaño hasta 20 mm tratadas en el Instituto Oncológico "Dr. Luis Razzeti", entre enero 2000 y diciembre de 2003, determinándose factores que influyen en la aparición de metástasis axilares, realizándose análisis de uni y multivariables. RESULTADOS: El trabajo consistió en una población de 121 pacientes, con una edad media de 57 años, 50 (41,32 %) que presentaron metástasis ganglionar axilar; los factores que se relacionaron con ganglios axilares positivos en el análisis de univariables fueron: grado histológico y nuclear, invasión linfovascular, índice mitótico elevado y tumores aneuploides (P Abstract in english OBJECTIVES: To identify and study that existing pathologically factors and clinical predict nodal metastases axillaries in mammary tumors with diameter size more and minor of 20 mm, so demonstrated in the axillaries dissection is an unnecessary procedure in most of these patients, and can omit with [...] surely in patients with favorable predictive factors. METHODS: We realize a retrospective study, review the macroscopic and microscopic reports, and the inmunohistochemestry in the cellular blocks of patients with breast carcinoma with size until 20 mm treated in the Oncology Institute "Dr. Luis Razetti", between January and December of 2000 - 2003, determining factors that influence in the appearance of lymph nodes axillaries positives, making unvaried and multivariate analysis. RESULTS: These work consisted in the studied of 121 patients with mean age 57 years, 50 (41, 32 %) presented axillaries disease nodes metastases; the factors that were related to positive lymph nodes in the unavailable analysis were: histological and nuclear grade, linfovascular invasion, mitotic index high, aneuploid tumors (P

Godoy Briceño, Alí Josué; Betancourt, Luís; Parada, David; Morales, Sergio Osorio.

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Reactivity of monoclonal antibodies to Rickettsia rickettsii with spotted fever and typhus group rickettsiae.  

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Analysis of 15 spotted fever group (SFG) and 2 typhus group strains of rickettsiae with a panel of monoclonal antibodies revealed a number of shared and unique epitopes of the 120- and 155-kilodalton surface proteins. All of the SFG strains but neither of the typhus group strains reacted with antibody to the lipopolysaccharidelike antigen of Rickettsia rickettsii; possibly the lipopolysaccharidelike antigen is the common antigen which defines the SFG. North Carolina and Montana strains of R. ...

Anacker, R. L.; Mann, R. E.; Gonzales, C.

1987-01-01

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Proposal to create subspecies of Rickettsia conorii based on multi-locus sequence typing and an emended description of Rickettsia conorii  

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Abstract Background Rickettsiae closely related to the Malish strain, the reference Rickettsia conorii strain, include Indian tick typhus rickettsia (ITTR), Israeli spotted fever rickettsia (ISFR), and Astrakhan fever rickettsia (AFR). Although closely related genotypically, they are distinct serotypically. Using multilocus sequence typing (MLST), we have recently found that distinct serotypes may not always represent distinct species within the Rickettsia g...

Zhu Yong; Fournier Pierre-Edouard; Eremeeva Marina; Raoult Didier

2005-01-01

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Antibodies against rickettsiae from spotted fever groups in horses from two mesoregions in the state of Santa Catarina, Brazil / Anticorpos contra rickettsias do grupo da febre maculosa em equinos de duas mesorregiões de Santa Catarina, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Bactérias do gênero Rickettsia são agentes da Febre Maculosa Brasileira (FMB), uma doença zoonótica, de difícil diagnóstico, rápida evolução e que pode levar o indivíduo à morte. Anticorpos contra Rickettsia spp. em equinos foram pesquisados, por meio da Reação de Imunofluorescência Indireta (RIFI&# [...] 8805;64), em 150 amostras de sangue colhidas de animais em duas mesorregiões de Santa Catarina (Planalto Serrano e Vale do Itajaí). A ocorrência de anticorpos contra Rickettsia spp. observada em equinos de duas mesorregiões de Santa Catarina foi de 18,66%, ocorrendo reações cruzadas em todas as amostras positivas para, no mínimo, duas das espécies testadas. Isoladamente, de acordo com as espécies, 25 (16,66%) amostras foram positivas para R. rickettsii, 15 (10%) para R. parkeri, 22 (14,66%) para R. amblyommii, 23 (15,33%) para R. rhipicephali, 16 (10,66%) para R. bellii e 19 (12,66%) para R. felis. Somente dois animais resultaram em um sorodiagnóstico conclusivo, um para Rickettsia bellii e outro para R. rickettsii, nas diluições máximas de 1:4096 e 1:512, respectivamente. A ocorrência de anticorpos contra Rickettsia spp. em equinos de duas mesorregiões de Santa Catarina indica a circulação de agentes da FMB nestes animais sentinela e ratificam a importância do estudo da febre maculosa no estado de Santa Catarina. Abstract in english Bacteria of the Rickettsia genus are agents of Brazilian Spotted Fever (BSF), a zoonotic disease which is difficult to diagnose, evolves quickly and can result in death. Antibodies against Rickettsia spp. in horses were studied, by means of Indirect Immunofluorescence Assay (IFAT ?64), in 150 [...] blood samples taken from animals in two Santa Catarina mesoregions (Planalto Serrano and Vale do Itajaí). The overall occurrence of Rickettsia spp. antibodies in horses was 18.66%, with cross-reactivity occurring in all positive samples for at least two of the species tested. Separately, according to the species, 25 (16.66%) samples were positive for R. rickettsii, 15 (10%) for R. parkeri, 22 (14.66%) for R. amblyommii, 23 (15.33%) for R. rhipicephali, 16 (10.66%) for R. bellii and 19 (12.66%) for R. felis. Only two animals resulted in a conclusive serodiagnosis, one for R. bellii and the other for R. rickettsii, at maximum dilutions of 1:4096 and 1:512, respectively. The occurrence of antibodies against Rickettsia spp. in horses from two mesoregions in the state of Santa Catarina indicates the movement of BSF agents in these sentinel animals and confirms the importance of studying spotted fever in the state of Santa Catarina.

Medeiros, A.P.; Moura, A.B.; Souza, A.P.; Bellato, V.; Sartor, A.A.; Vieira-Neto, A.; Moraes-Filho, J.; Labruna, M.B..

 
 
 
 
81

First report on the occurrence of Rickettsia slovaca and Rickettsia raoultii in Dermacentor silvarum in China  

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Full Text Available Abstract Background Rickettsioses are among both the longest known and most recently recognized infectious diseases. Although new spotted fever group rickettsiae have been isolated in many parts of the world including China, Little is known about the epidemiology of Rickettsia pathogens in ticks from Xinjiang Autonomous Region of China. Methods In an attempt to assess the potential risk of rickettsial infection after exposure to ticks in Xinjiang Uygur Autonomous Region of China, a total of 200 Dermacentor silvarum ticks collected in Xinyuan district were screened by polymerase chain reaction based on the outer membrane protein A gene. Results 22 of the 200 specimens (11% were found to be positive by PCR. Phylogenetic analysis of OmpA sequences identified two rickettsial species, Rickettsia raoultii (4.5% and Rickettsia slovaca (6.5%. Conclusions This study has reported the occurrence of Rickettsia raoultii and Rickettsia slovaca in Xinjiang Autonomous Region of China and suggests that Dermacentor silvarum could be involved in the transmission of rickettsial agents in China. Further studies on the characterization and culture of rickettsial species found in Dermacentor silvarum should be performed to further clarify this. Additionally, the screening of human specimens for rickettsial disease in this region will define the incidence of infection.

Tian Zhan-Cheng

2012-01-01

82

In Vitro Activities of Telithromycin (HMR 3647) against Rickettsia rickettsii, Rickettsia conorii, Rickettsia africae, Rickettsia typhi, Rickettsia prowazekii, Coxiella burnetii, Bartonella henselae, Bartonella quintana, Bartonella bacilliformis, and Ehrlichia chaffeensis  

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In vitro activities of telithromycin compared to those of erythromycin against Rickettsia spp., Bartonella spp., Coxiella burnetii, and Ehrlichia chaffeensis were determined. Telithromycin was more active than erythromycin against Rickettsia, Bartonella, and Coxiella burnetii, with MICs of 0.5 ?g/ml, 0.003 to 0.015 ?g/ml, and 1 ?g/ml, respectively, but was inactive against Ehrlichia chaffeensis.

Rolain, Jean-marc; Maurin, Max; Bryskier, Andre?; Raoult, Didier

2000-01-01

83

Human Infections with Rickettsia raoultii, China.  

Science.gov (United States)

We used molecular methods to identify Rickettsia raoultii infections in 2 persons in China. These persons had localized rashes around sites of tick bites. R. raoultii DNA was detected in 4% of Dermacentor silvarum ticks collected in the same area of China and in 1 feeding tick detached from 1 patient. PMID:24750663

Jia, Na; Zheng, Yuan-Chun; Ma, Lan; Huo, Qiu-Bo; Ni, Xue-Bing; Jiang, Bao-Gui; Chu, Yan-Li; Jiang, Rui-Ruo; Jiang, Jia-Fu; Cao, Wu-Chun

2014-05-01

84

Rickettsia felis-associated uneruptive fever, Senegal.  

Science.gov (United States)

During November 2008-July 2009, we investigated the origin of unknown fever in Senegalese patients with a negative malaria test result, focusing on potential rickettsial infection. Using molecular tools, we found evidence for Rickettsia felis-associated illness in the initial days of infection in febrile Senegalese patients without malaria. PMID:20587190

Socolovschi, Cristina; Mediannikov, Oleg; Sokhna, Cheikh; Tall, Adama; Diatta, Georges; Bassene, Hubert; Trape, Jean François; Raoult, Didier

2010-07-01

85

Rickettsia felis–associated Uneruptive Fever, Senegal  

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During November 2008–July 2009, we investigated the origin of unknown fever in Senegalese patients with a negative malaria test result, focusing on potential rickettsial infection. Using molecular tools, we found evidence for Rickettsia felis–associated illness in the initial days of infection in febrile Senegalese patients without malaria.

2010-01-01

86

Rickettsia slovaca infection in humans, Portugal.  

Science.gov (United States)

Fifteen years after the initial detection of Rickettsia slovaca in ticks in Portugal, 3 autochthonous cases of R. slovaca infection were diagnosed in humans. All patients had an eschar on the scalp and lymphadenopathy; 2 patients had facial edema. R. slovaca infection was confirmed by serologic testing, culture, and PCR. PMID:24050379

de Sousa, Rita; Pereira, Branca Isabel; Nazareth, Claúdia; Cabral, Susana; Ventura, Conceição; Crespo, Pedro; Marques, Nuno; da Cunha, Saraiva

2013-10-01

87

Bartonella quintana and Rickettsia felis in Gabon  

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We detected Rickettsia felis DNA in Ctenocephalides felis and Bartonella quintana DNA in 3 Pulex irritans fleas taken from a pet Cercopithecus cephus monkey in Gabon, sub-Saharan Africa. This is the first report of B. quintana in the human flea.

Rolain, Jean-marc; Bourry, Olivier; Davoust, Bernard; Raoult, Didier

2005-01-01

88

Rickettsia felis in Fleas, Western Australia  

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This study is the first confirmation of Rickettsia felis in Australia. The organism was identified from 4 species of fleas obtained from dogs and cats in Western Australia, by using polymerase chain reaction amplification and DNA sequencing of the citrate synthase and outer membrane protein A genes.

2006-01-01

89

A Plaque Assay for Rickettsia Rickettsii.  

Science.gov (United States)

A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or African green monkey kidney (VERO) monolayer cell cultures with either an agarose or a special Noble agar overlay. Plaques were counted after 6 day...

E. H. Weinberg J. R. Stakebake P. J. Gerone

1969-01-01

90

Rickettsia felis in Xenopsylla cheopis, Java, Indonesia  

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Rickettsia typhi and R. felis, etiologic agents of murine typhus and fleaborne spotted fever, respectively, were detected in Oriental rat fleas (Xenopsylla cheopis) collected from rodents and shrews in Java, Indonesia. We describe the first evidence of R. felis in Indonesia and naturally occurring R. felis in Oriental rat fleas.

Jiang, Ju; Soeatmadji, Djoko W.; Henry, Katherine M.; Ratiwayanto, Sutanti; Bangs, Michael J.; Richards, Allen L.

2006-01-01

91

Human Infections with Rickettsia raoultii, China  

Science.gov (United States)

We used molecular methods to identify Rickettsia raoultii infections in 2 persons in China. These persons had localized rashes around sites of tick bites. R. raoultii DNA was detected in 4% of Dermacentor silvarum ticks collected in the same area of China and in 1 feeding tick detached from 1 patient.

Jia, Na; Zheng, Yuan-Chun; Ma, Lan; Huo, Qiu-Bo; Ni, Xue-Bing; Jiang, Bao-Gui; Chu, Yan-Li; Jiang, Rui-Ruo; Jiang, Jia-Fu

2014-01-01

92

Rickettsia infection in dogs and Rickettsia parkeri in Amblyomma tigrinum ticks, Cochabamba Department, Bolivia.  

Science.gov (United States)

Only few published data are available on ticks and tick-borne zoonotic pathogens in Bolivia. To evaluate rickettsial seroprevalence and infection in dogs and ticks, during February-April 2007, we collected whole blood, sera, and ticks from dogs living in the rural, peri-urban, and urban areas of Cochabamba, Bolivia. Dog sera were subjected to enzyme-linked immunosorbent assay test to detect IgG antibodies against Rickettsia rickettsii and 68.2% of samples were found to be positive (n?=?30; 95% confidence interval [CI]: 52.4-81.4). Blood samples and ticks were tested using polymerase chain reaction to detect spotted fever group (SFG) rickettsiae. One blood sample was positive for Rickettsia parkeri (2.3%; 95% CI: 0.06-12.3). Ticks were collected from 10 dogs and were identified as Amblyomma tigrinum (n?=?44) and Rhipicephalus sanguineus (n?=?1). All A. tigrinum ticks were collected from resident dogs from the rural areas of Cochabamba, whereas R. sanguineus was from a dog originating from Santa Cruz. Of 42 DNA samples extracted from ticks, 23 (54.8%; 95% CI: 38.7-70.1) were polymerase chain reaction positive for Rickettsia spp. Sequencing analysis identified 22 samples as R. parkeri and one as Rickettsia aeschlimannii. Positive ticks (all A. tigrinum) were collected from six dogs, all of which were seropositive. This is the first report of SFG rickettsiae in A. tigrinum, suggesting that this tick-like others species in the Amblyomma maculatum group--may play a role in the biological cycle of Ri. parkeri. The high infection prevalence of SFG rickettsiae in ticks and the even higher seroprevalence in dogs suggest an active circulation of agents of rickettsiosis in the study area, although there are no confirmed cases of infection in humans. Our study supports the use of canine serology as risk indicator for SF rickettsioses. PMID:20426684

Tomassone, Laura; Conte, Valeria; Parrilla, Guillermo; De Meneghi, Daniele

2010-12-01

93

Improved plaque assays for Rickettsia prowazekii in Vero 76 cells.  

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Typhus group rickettsiae, including Rickettsia prowazekii and R. typhi, produce visible plaques on primary chick embryo fibroblasts and low-passage mouse embryo fibroblasts but do not form reproducible plaques on continuous cell culture lines. We tested medium overlay modifications for plaque formation of typhus group rickettsiae on the continuous fibroblast cell line Vero76. A procedure involving primary overlay with medium at pH 6.8, which was followed 2 to 3 days later with secondary overl...

Policastro, P. F.; Peacock, M. G.; Hackstadt, T.

1996-01-01

94

[Serological study of rickettsia infections in Niamey, Niger].  

Science.gov (United States)

Rickettsioses is a possible alternative to presumptive diagnosis of malaria. A serologic study was carried out in 1994 to determine the prevalence of rickettsioses in children under 5 years of age from three different areas of Niamey, Niger. Indirect immunofluorescent assays using the micromethod were performed with antigens for Rickettsia conori, Rickettsia mooseri, and Coxiella burneti. Results were read from a positive threshold of 1/160 up to 1/640. Out of a randomized population of 177 children 35 were positive for at least one antigen: 17.5% for Rickettsia conori, 15.8% for Rickettsia mooseri, and 9.6% for Coxiella burneti. The incidence of positivity for Rickettsia mooseri and Coxiella burneti. was significantly higher in an area where contact between people and animals was particularly close. This high rate of positivity is in agreement with previous reports in other countries in West Africa and suggests that close contact between man and rickettsiae is common. Although dogs carry ticks in Niger, direct contact with Rickettsia conori is probably the most mode of transmission. Rodents like Cricetomys gambianus and Rattus norvegicus carry Rickettsia mooseri and goats and sheep which are often kept in the courtyards of buildings carry Coxiella burneti. The recently identified species Rickettsia africae could be transmitted by other vectors such as cattle ticks. PMID:9304008

Julvez, J; Michault, A; Kerdelhue, C

1997-01-01

95

Detection of "Rickettsia sp. strain Uilenbergi" and "Rickettsia sp. strain Davousti" in Amblyomma tholloni ticks from elephants in Africa  

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Full Text Available Abstract Background To date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in Africa and 4 of them were first identified in ticks before being recognized as human pathogens. Results We examined 33 and 5 Amblyomma tholloni ticks from African elephants in the Central African Republic and Gabon, respectively, by PCR amplification and sequencing of a part of gltA and ompA genes of the genus Rickettsia. The partial sequences of gltA and ompA genes detected in tick in Gabon had 99.1% similarity with those of R. heilongjiangensis and 97.1% with those of Rickettsia sp. HL-93 strain, respectively. The partial gltA and ompA gene sequences detected in tick in the Central African Republic were 98.9% and 95.1% similar to those of Rickettsia sp. DnS14 strain and R. massiliae, respectively. Phylogenetic analysis showed Rickettsia sp. detected in Gabon clusters with R. japonica and R. heilongjiangensis in a phylogenetic tree based on the partial gltA and ompA genes. The genotype of the Rickettsia sp. detected in the Central African Republic is close to those of R. massiliae group in the phylogenetic tree based on partial gltA gene sequences, and distantly related to other rickettsiae in the tree based on partial ompA gene. Conclusion The degrees of similarity of partial gltA and ompA genes with recognized species indicate the rickettsiae detected in this study may be new species although we could only study the partial sequences of 2 genes regarding the amount of DNA that was available. We propose the Rickettsia sp. detected in Gabon be provisionally named "Rickettsia sp. stain Davousti" and Rickettsia sp. detected in the Central African Republic be named "Rickettsia sp. strain Uilenbergi".

Jeffery Kathryn

2007-08-01

96

Isolation of species-specific protein antigens of Rickettsia typhi and Rickettsia prowazekii for immunodiagnosis and immunoprophylaxis.  

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A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or detergent-treated rickettsiae, as measured by rocket immunoelectrophoresis, other polypeptides were also present, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophor...

Dasch, G. A.

1981-01-01

97

Comparación de la Tomografía Axial Computarizada con el estudio anatomopatológico en el estadiaje ganglionar del cáncer de pulmón  

Scientific Electronic Library Online (English)

Full Text Available SciELO Spain | Language: Spanish Abstract in spanish PROPÓSITO: Evaluar la Sensibilidad, Especificidad, Valor predictivo positivo y Valor Predictivo Negativo de la Tomografia Axial Computarizada (TAC) en el estadiaje ganglionar del cáncer de pulmón. MATERIAL Y MÉTODOS: Se han analizado 30 pacientes diagnosticados de carcinoma pulmonar entre Mayo de 20 [...] 03 y Mayo de 2004. A todos los pacientes se les realizó una TAC torácica, valorando la afectación ganglionar mediastínica. A su vez, se les realizó o una mediastinoscopia o una resección pulmonar más linfadenectomía mediastínica, obteniendo así material para el estudio anatomo-patológico para confirmar o no la afectación ganglionar mediastínica y correlacionarla con los hallazgos de la TAC. RESULTADOS: Hemos obtenido una Sensibilidad del 72,2%, una Especificidad del 100%, un valor predictivo positivo del 100% y un valor predictivo negativo del 70,6% para la TAC, utilizando como "gold standar" el estudio anatomopatológico. CONCLUSIONES: La TAC torácica se considera una prueba de imagen de rutina en el diagnóstico del cáncer de pulmón; pero en muchos casos no es capaz de estadiar correctamente la afectación ganglionar mediastínica. Es en estos casos, donde es necesario realizar pruebas invasivas como la mediastinoscopia. Actualmente, la aparición de la PET permite estadiar mejor el tumor, ofreciendo mejor tratamiento a cada paciente, y en determinados casos evitar técnicas diagnósticas invasivas. Abstract in english PURPOSE: To analise the sensitivity, specificity, and positive and negative predictive values of the computerized axial tomography (CT) in nodal stages of lung carcinoma. MATERIAL AND METHODS: Thirty patients suffering from lung carcinoma were analysed between May 2003 and May 2004. CT screening of [...] the thorax was made to all the patients. Mediastinoscopy or lung resection samples plus systematic node dissection were performed, and the efficiency of CT analysed by comparing the obtained data with the histopathology results of nodal affection shown by mediastinoscopy and lymph node dissection. RESULTS: The sensitivity, specificity, and positive and negative predictive value of the computerized axial tomography (CT) for nodal staging involved in lung carcinoma were 72.2%, 100%, 100% and 70,6% respectively. CONCLUSIONS: CT of the thorax is considered a usual imaging technique in lung carcinoma diagnosis; but in some cases it does not focalise correctly the nodal staging involved in lung carcinoma. In theses cases, it is necessarry to perform invasive techniques such as mediastinoscopy. At present, positron emission tomography (PET) is the technique that better permits to focalise the tumor offering the best data for the therapy of each patient, and avoiding invasive diagnosis techniques.

López Mata, M.; Valencia Julve, J.; Bascón Santaló, N.; Velilla Millán, C.; Escó Barón, R.; García Álvarez, M..

98

Serosurvey of Rickettsia typhi and Rickettsia felis in HIV-infected patients.  

Science.gov (United States)

Consistent with the effects of HIV on cell-mediated immunity, an increased susceptibility to intracellular microorganisms has been observed. Rickettsiae are obligate intracellular microorganisms. The aim of this study was to examine Rickettsia typhi and Rickettsia felis infections in HIV+ population. Sera of 341 HIV+ patients were evaluated by indirect immunofluorescent assay. Age, sex, residential locality, risk behavior, stage according to criteria of the Center for Disease Control and Prevention, CD4+/CD8+ T cells, Hepatitis B antigen, and Hepatitis C serology were surveyed. Seroprevalences of R. typhi and R. felis infection were 7.6% and 4.4%, respectively. No associations were found between seropositivities and the assessed variables. Findings were similar to those obtained in healthy subjects from the same region. PMID:24467705

Nogueras, María Mercedes; Pons, Immaculada; Sanfeliu, Isabel; Sala, Montserrat; Segura, Ferran

2014-04-01

99

Permeability of Rickettsia prowazekii to NAD  

International Nuclear Information System (INIS)

Rickettsia prowazekii accumulated radioactivity from [adenine-2,8-3H]NAD but not from [nicotinamide-4-3H]NAD, which demonstrated that NAD was not taken up intact. Extracellular NAD was hydrolyzed by rickettsiae with the products of hydrolysis, nicotinamide mononucleotide and AMP, appearing in the incubation medium in a time- and temperature-dependent manner. The particulate (membrane) fraction contained 90% of this NAD pyrophosphatase activity. Rickettsiae which had accumulated radiolabel after incubation with [adenine-2,8-3H]NAD were extracted, and the intracellular composition was analyzed by chromatography. The cells contained labeled AMP, ADP, ATP, and NAD. The NAD-derived intracellular AMP was transported via a pathway distinct from and in addition to the previously described AMP translocase. Exogenous AMP (1 mM) inhibited uptake of radioactivity from [adenine-2,8-3H]NAD and hydrolysis of extracellular NAD. AMP increased the percentage of intracellular radiolabel present as NAD. Nicotinamide mononucleotide was not taken up by the rickettsiae, did not inhibit hydrolysis of extracellular NAD, and was not a good inhibitor of the uptake of radiolabel from [adenine-2,8-3H]NAD. Neither AMP nor ATP (both of which are transported) could support the synthesis of intracellular NAD. The presence of intracellular [adenine-2,8-3H]NAD within an organism in which intact NAD could not be transported suggested the resynthesis from AMP of [adenine-2,8-3H]NAD at the locus of NAD hydrolysis and translocation

1989-01-01

100

Permeability of Rickettsia prowazekii to NAD  

Energy Technology Data Exchange (ETDEWEB)

Rickettsia prowazekii accumulated radioactivity from (adenine-2,8-3H)NAD but not from (nicotinamide-4-3H)NAD, which demonstrated that NAD was not taken up intact. Extracellular NAD was hydrolyzed by rickettsiae with the products of hydrolysis, nicotinamide mononucleotide and AMP, appearing in the incubation medium in a time- and temperature-dependent manner. The particulate (membrane) fraction contained 90% of this NAD pyrophosphatase activity. Rickettsiae which had accumulated radiolabel after incubation with (adenine-2,8-3H)NAD were extracted, and the intracellular composition was analyzed by chromatography. The cells contained labeled AMP, ADP, ATP, and NAD. The NAD-derived intracellular AMP was transported via a pathway distinct from and in addition to the previously described AMP translocase. Exogenous AMP (1 mM) inhibited uptake of radioactivity from (adenine-2,8-3H)NAD and hydrolysis of extracellular NAD. AMP increased the percentage of intracellular radiolabel present as NAD. Nicotinamide mononucleotide was not taken up by the rickettsiae, did not inhibit hydrolysis of extracellular NAD, and was not a good inhibitor of the uptake of radiolabel from (adenine-2,8-3H)NAD. Neither AMP nor ATP (both of which are transported) could support the synthesis of intracellular NAD. The presence of intracellular (adenine-2,8-3H)NAD within an organism in which intact NAD could not be transported suggested the resynthesis from AMP of (adenine-2,8-3H)NAD at the locus of NAD hydrolysis and translocation.

Atkinson, W.H.; Winkler, H.H.

1989-02-01

 
 
 
 
101

Race and Rickettsiae: A United States Perspective  

Digital Repository Infrastructure Vision for European Research (DRIVER)

US surveillance programs for Rocky Mountain spotted fever (RMSF), ehrlichiosis, and anaplasmosis collect demographic data on patients, including race and ethnicity. Reporting of these diseases among race groups is not uniform across the United States. Because a laboratory confirmation is required to meet the national surveillance case definition, reporting may be influenced by a patient's access to healthcare. Determining the association between race and ethnicity with incidence of rickettsia...

Dahlgren, F. Scott; Moonesinghe, Ramal; Mcquiston, Jennifer H.

2011-01-01

102

In vitro susceptibilities of Coxiella burnetii, Rickettsia rickettsii, and Rickettsia conorii to the fluoroquinolone sparfloxacin.  

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In vitro susceptibilities of Rickettsia rickettsii, Rickettsia conorii, and Coxiella burnetii to the new fluoroquinolone sparfloxacin (AT-4140; RP 64206) were determined. Plaque and dye uptake assays were used to measure the MICs against R. rickettsii and R. conorii. The susceptibilities of C. burnetii Nine Mile and Q 212 were determined in two acute-infection models and in two chronic-infection models. The MICs were 0.125 to 0.25 microgram/ml for R. rickettsii and 0.25 to 0.5 microgram/ml fo...

Raoult, D.; Bres, P.; Drancourt, M.; Vestris, G.

1991-01-01

103

Prevalence of Rickettsia Species in Canadian Populations of Dermacentor andersoni and D. variabilis?  

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We determined the prevalence of rickettsiae in Dermacentor adults at 15 localities in Canada. Rickettsia rickettsii was not detected in any tick, whereas Rickettsia peacockii was present in 76% of Dermacentor andersoni adults and Rickettsia montanensis in 8% of Dermacentor variabilis adults. This host specificity was maintained in localities where both tick species occurred in sympatry.

Dergousoff, Shaun J.; Gajadhar, Andrew J. A.; Chilton, Neil B.

2009-01-01

104

Simultaneous Detection of “Rickettsia mongolotimonae” in a Patient and in a Tick in Greece  

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Rickettsia conorii, a spotted fever group rickettsia which is transmitted by Rhipicephalus sp. complex ticks, was considered until now the only pathogenic rickettsia prevalent in Greece. Here, we report the presence of “Rickettsia mongolotimonae” (proposed name) detected simultaneously in a patient and in a Hyalomma anatolicum excavatum tick, sampled on the patient.

Psaroulaki, Anna; Germanakis, Antonis; Gikas, Achilleas; Scoulica, Efstathia; Tselentis, Yannis

2005-01-01

105

Simultaneous Detection of "Rickettsia mongolotimonae" in a Patient and in a Tick in Greece  

Science.gov (United States)

Rickettsia conorii, a spotted fever group rickettsia which is transmitted by Rhipicephalus sp. complex ticks, was considered until now the only pathogenic rickettsia prevalent in Greece. Here, we report the presence of “Rickettsia mongolotimonae” (proposed name) detected simultaneously in a patient and in a Hyalomma anatolicum excavatum tick, sampled on the patient.

Psaroulaki, Anna; Germanakis, Antonis; Gikas, Achilleas; Scoulica, Efstathia; Tselentis, Yannis

2005-01-01

106

Detecting Rickettsia parkeri Infection from Eschar Swab Specimens  

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The typical clinical presentation of several spotted fever group Rickettsia infections includes eschars. Clinical diagnosis of the condition is usually made by analysis of blood samples. We describe a more sensitive, noninvasive means of obtaining a sample for diagnosis by using an eschar swab specimen from patients infected with Rickettsia parkeri.

Myers, Todd; Lalani, Tahaniyat; Dent, Mike; Jiang, Ju; Daly, Patrick L.; Maguire, Jason D.; Richards, Allen L.

2013-01-01

107

The First Human Case of Rickettsia tamurae Infection in Japan  

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A case of Rickettsia tamurae infection in Japan is reported. A 76-year-old Japanese male had a tick bite which developed to local skin inflammation on his left leg. Anti-rickettsia antibodies were detected in his serum, and R. tamurae DNA was identified in his blood, the lesional skin, and the tick.

Imaoka, Kaoru; Kaneko, Sakae; Tabara, Kenji; Kusatake, Kenji; Morita, Eishin

2011-01-01

108

Sequence and Annotation of Rickettsia sibirica sibirica Genome  

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Rickettsia sibirica sibirica is the causative agent of Siberian or North Asian tick typhus, a tick-borne rickettsiosis known to exist in Siberia and eastern China. Here we present the draft genome of Rickettsia sibirica sibirica strain BJ-90 isolated from Dermacentor sinicus ticks collected in Beijing, China.

Sentausa, Erwin; El Karkouri, Khalid; Robert, Catherine; Raoult, Didier; Fournier, Pierre-edouard

2012-01-01

109

High Seroprevalence for Typhus Group Rickettsiae, Southwestern Tanzania  

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Rickettsioses caused by typhus group rickettsiae have been reported in various African regions. We conducted a cross-sectional survey of 1,227 participants from 9 different sites in the Mbeya region, Tanzania; overall seroprevalence of typhus group rickettsiae was 9.3%. Risk factors identified in multivariable analysis included low vegetation density and highway proximity.

Dill, Tatjana; Dobler, Gerhard; Saathoff, Elmar; Clowes, Petra; Kroidl, Inge; Ntinginya, Elias; Machibya, Harun; Maboko, Leonard; Lo?scher, Thomas; Hoelscher, Michael; Heinrich, Norbert

2013-01-01

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Rickettsiae in questing Ixodes ricinus ticks in the Czech Republic.  

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Tick-borne rickettsiae are an important topic in the field of emerging infectious diseases. In the study, we screened a total of 1473 field-collected Ixodes ricinus ticks (1294 nymphs, 99 males, and 80 females) for the presence of human pathogenic rickettsiae (Rickettsia helvetica, R. monacensis, 'Candidatus Neoehrlichia mikurensis', and Anaplasma phagocytophilum) in natural and urban ecosystems using molecular techniques. The minimum infection rate (MIR) for Rickettsia spp. was found to be 2.9% in an urban park and 3.4% in a natural forest ecosystem; for 'Candidatus Neoehrlichia mikurensis', we observed MIRs of 0.4% in the city park and 4.4% in the natural habitat, while for A. phagocytophilum the MIR was 9.4% and 1.9%, respectively. Our study provides the first data on the occurrence of human pathogenic rickettsiae in questing I. ricinus ticks in the Czech Republic. PMID:24252265

Venclikova, Kristyna; Rudolf, Ivo; Mendel, Jan; Betasova, Lenka; Hubalek, Zdenek

2014-03-01

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Serological cross-reaction and cross-protection in guinea pigs infected with Rickettsia rickettsii and Rickettsia montana.  

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Antisera produced in guinea pigs inoculated with Rickettsia rickettsii or Rickettsia montana were cross-reactive but sufficiently specific to identify the primary infecting agent. Guinea pigs immunized with R. montana were protected from fatal infection with R. rickettsii, although a few (25%) developed mild fever of short duration.

Feng, W. C.; Waner, J. L.

1980-01-01

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Actualización en metástasis ganglionar de carcinoma escamoso de cabeza y cuello: Disección ganglionar, ganglio centinela y técnicas de biología molecular Update in lymph node metastasis from head and neck squamous cell carcinoma: Lymph node dissection, sentinel lymph node and molecular biology techniques  

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Full Text Available El carcinoma escamoso es la principal neoplasia maligna de cabeza y cuello en los adultos. Esta neoplasia se origina en la mucosa del tracto aerodigestivo superior. Se discute su extensión en superficie y las metástasis a ganglios linfáticos cervicales. El compromiso ganglionar es el principal factor pronóstico independiente del carcinoma escamoso de cabeza y cuello, pues la presencia de adenopatías metastásicas reduce la sobrevida casi en 50%. La siguiente revisión se centra en tres temas relacionados con las metástasis ganglionares en carcinoma escamoso de cabeza y cuello (CECC: la clasificación de niveles ganglionares y de la disección ganglionar cervical, la técnica del ganglio centinela en el CECC y las técnicas de biología molecular para el diagnóstico del compromiso tumoral ganglionarSquamous cell carcinoma is the main head and neck malignant cancer in adults. This cancer originates from the upper aero digestive tract mucosa. Its surface extension and cervical lymph node metastases are discussed. Lymph node involvement is the main independent prognostic factor in head and neck squamous cell carcinoma, given that the presence of metastatic nodes reduce survival by approximately 50%. The present review focus on three topics related to lymph nodes metastasis of head and neck squamous cell carcinomas (CECC: Classification of lymph node levels and cervical node dissection, the sentinel lymph node technique in CECC, and the use of molecular biology techniques for diagnosing lymph node involvement

Pablo Ortega R

2008-04-01

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Molecular detection of Rickettsia bellii and Rickettsia sp. strain Colombianensi in ticks from Cordoba, Colombia.  

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The purpose of this study was to provide molecular evidence of Rickettsia spp. in ticks collected from 2 sites of Cordoba. From May to June 2009, 1069 Amblyomma cajennense ticks were removed from 40 capybaras (Hydrochoerus hydrochaeris) in a rural locality of Monteria. Furthermore, 458 Amblyomma sp. larvae and 20 Amblyomma sp. nymphs were collected in a rural locality of Los Cordobas (Cordoba) by drag sampling on vegetation (n=1547). Ticks were grouped into pools and tested for rickettsial infection by real-time PCR targeting the rickettsial gene gltA. Subsequently, PCR targeting for gltA, ompA, ompB, and 16S rRNA, sequencing, and phylogenetic analyses were undertaken. Rickettsial DNA was detected in 10 (4.6%) out of 214 pools of ticks by RT-PCR. Five (33%) of free-living Amblyomma sp. larval pools were positive, as well as 5 (2.6%) pools from A. cajennense. Only the gltA gene was amplified from 5 pools of free-living larvae. The nucleotide sequences were 100% identical to R. bellii by BLAST. Only one pool from A. cajennense was positive for gltA, ompA, ompB, and 16S rRNA. The partial nucleotide sequences of these genes were 100% identical to nucleotide sequences of the same genes of a new proposed species Candidatus Rickettsia sp. strain Colombianensi. This is the first report of R. bellii in ticks in Colombia and the second report of detection of Candidatus Rickettsia sp. strain Colombianensi. These Rickettsia species are still considered of unknown pathogenicity. Further studies are needed to characterize the ecological and potential pathogenic role of these 2 Rickettsia species found in Cordoba. PMID:24378078

Miranda, Jorge; Mattar, Salim

2014-03-01

114

Tick-borne rickettsiae in Guinea and Liberia.  

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While the high seroprevalence for the rickettsiae that cause spotted fevers and the multiple pathogenic rickettsiae is known, the data on the distribution of rickettsial diseases in Africa are often incomplete. We collected ticks from domestic or wild animals (generally a source of bushmeat) that were in contact with humans in 2 neighboring countries of tropical West Africa, Guinea and Liberia. In total, 382 ticks representing 6 species were collected in Liberia and 655 ticks representing 7 species were collected in Guinea. We found rickettsiae in 9 different species of ticks from both countries. Rickettsia africae was found in 93-100% of Amblyomma variegatum, in 14-93% of Rhipicephalus (B.) geigyi, Rh. (B.) annulatus, and Rh. (B.) decoloratus, and in several Hyalomma marginatum rufipes and Haemaphysalis paraleachi. A genetic variant of R. africae was found in Amblyomma compressum. R. massiliae was found in 10/61 (16%) of Rh. senegalensis ticks and in 2% of Haemaphysalis paraleachi ticks collected from dogs. We identified a new rickettsia in one of 44 (2%) Ixodes muniensis collected from a dog in Liberia. As this rickettsia is not yet isolated, we propose the provisional name "Candidatus Rickettsia liberiensis" (for the West African country where the host tick was collected). PMID:22309858

Mediannikov, Oleg; Diatta, Georges; Zolia, Yah; Balde, Mamadou Cellou; Kohar, Henry; Trape, Jean-François; Raoult, Didier

2012-02-01

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Rickettsia monacensis sp. nov., a Spotted Fever Group Rickettsia, from Ticks (Ixodes ricinus) Collected in a European City Park  

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We describe the isolation and characterization of Rickettsia monacensis sp. nov. (type strain, IrR/MunichT) from an Ixodes ricinus tick collected in a city park, the English Garden in Munich, Germany. Rickettsiae were propagated in vitro with Ixodes scapularis cell line ISE6. BLAST analysis of the 16S rRNA, the citrate synthase, and the partial 190-kDa rickettsial outer membrane protein A (rOmpA) gene sequences demonstrated that the isolate was a spotted fever group (SFG) rickettsia closely r...

Simser, Jason A.; Palmer, Ann T.; Fingerle, Volker; Wilske, Bettina; Kurtti, Timothy J.; Munderloh, Ulrike G.

2002-01-01

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Detection of Rickettsia in Rhipicephalus sanguineus ticks and Ctenocephalides felis fleas from southeastern Tunisia by reverse line blot assay.  

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Ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern Tunisia were screened for Rickettsia infection using reverse line blot assay. Evidence of spotted fever group Rickettsia was obtained. We detected Rickettsia felis in fleas, Rickettsia massiliae Bar 29 and the Rickettsia conorii Israeli spotted fever strain in ticks, and Rickettsia conorii subsp. conorii and Rickettsia spp. in both arthropods. The sensitivity of the adopted technique allowed the identification of a new association between fleas and R. conorii subsp. conorii species. The presence of these vector-borne Rickettsia infections should be considered when diagnosing this disease in humans in Tunisia. PMID:24226919

Khrouf, Fatma; M'Ghirbi, Youmna; Znazen, Abir; Ben Jemaa, Mounir; Hammami, Adnene; Bouattour, Ali

2014-01-01

117

Spotted fever group rickettsiae in ticks in Turkey.  

Science.gov (United States)

One hundred twenty-six ticks belonging to 12 tick species were collected from humans, domestic and wild animals, and from the ground as unfed (questing ticks) from distinct localities in Turkey in 2011. Ticks were individually tested by polymerase chain reaction (PCR) for Rickettsia spp., amplifying citrate synthase (gltA), and outer membrane protein (ompA) genes. Twenty-five ticks (19.8%) were found to be infected with Rickettsia species. Five SFG rickettsiae were identified, including 4 pathogens: Ri. aeschlimannii in Hyalomma marginatum, Hy. aegyptium, Hyalomma sp. (nymph), and Rhipicephalus turanicus; Ri. africae in Hy. excavatum, Hy. aegyptium, and Hyalomma sp. (nymph); Ri. slovaca and Ri. raoultii in Dermacentor marginatus; and one species with unknown pathogenicity, Ri. hoogstraalii, in Haemaphysalis parva. Rickettsia slovaca and Ri. hoogstraalii were reported for the first time from Turkey. In addition, Ri. hoogstraalii and Ri. africae were detected for the first time in Ha. parva and Hy. excavatum ticks, respectively. PMID:24355764

Orkun, Ömer; Karaer, Zafer; Çakmak, Ay?e; Nalbanto?lu, Serpil

2014-03-01

118

Experimental Scrub Typhus Immunogens: Gamma-Irradiated and Formalinized Rickettsiae.  

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In this study, scrub typhus immunogens were prepared by inactivating infected yolk sac suspensions of Rickettsia tsutsugamushi through use of either formalin or a lethal dose of gamma radiation. Mouse protection tests showed that radiation-inactivated pre...

G. H. G. Eisenberg J. V. Osterman

1976-01-01

119

Comparação entre tomografia computadorizada e mediastinoscopia na avaliação do envolvimento ganglionar mediastínico no carcinoma brônquico não de pequenas células / Comparison between computed tomography and mediastinoscopy in the assessment of mediastinal nodal involvement in non-small cell bronchial carcinoma  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese OBJETIVO: Avaliar o rendimento da tomografia computadorizada torácica, em relação à mediastinoscopia, na detecção de metástases ganglionares mediastinais em pacientes portadores de carcinoma brônquico analisando o rendimento dessa e identificando as regiões mais problemáticas. MATERIAIS E MÉTODOS: A [...] nalisamos 195 pacientes portadores de carcinoma brônquico, buscando-se comparar os achados entre tomografia computadorizada torácica e mediastinoscopia com biópsia. RESULTADOS: Em relação às metástases nodais mediastinais, 33,9% tinham doença metastática ganglionar peribrônquica e/ou hilar ipsilateral, 46,1% possuíam metástases mediastinais ipsilaterais e/ou subcarinais e 20% apresentavam doença metastática mediastinal e/ou hilar contralateral, escalênica ou supraclavicular. As regiões com melhores valores de sensibilidade foram traqueobrônquica direita, paratraqueal direita alta e paratraqueal esquerda alta. As regiões nodais com melhores resultados de especificidade foram paratraqueal esquerda alta, paratraqueal direita alta e regiões traqueobrônquicas. CONCLUSÃO: A tomografia computadorizada torácica mostrou-se importante ferramenta diagnóstica na detecção de anormalidades em gânglios mediastinais; entretanto, a natureza neoplásica desses gânglios deve ser conferida por mediastinoscopia, ou até mesmo por toracotomia, a fim de que a correta decisão quanto ao tratamento possa ser tomada. Abstract in english OBJECTIVE: To evaluate the yielding of chest computed tomography in comparison with mediastinoscopy for detection of mediastinal nodal metastases in patients with bronchial carcinoma, and identifying the most problematic regions. MATERIALS AND METHODS: We have analyzed 195 patients with bronchial ca [...] rcinoma, comparing the findings of chest computed tomography and mediastinoscopy with biopsy. RESULTS: As regards mediastinal nodal metastasis, 33.9% of patients presented peribronchial and/or ipsilateral hilar nodal metastases, 46.1% ipsilateral and/or subcarinal mediastinal metastases, and 20% contralateral mediastinal and/or hilar, scalenic or supraclavicular metastatic disease. Higher sensitivity values were found in the following regions: right tracheobronchial, right upper paratracheal, and left upper paratracheal. Higher specificity values were found in the following nodal regions: left upper paratracheal, right upper paratracheal and tracheobronchial. CONCLUSION: Chest computed tomography has shown to be an important diagnostic tool for detection of mediastinal lymph nodes abnormalities. However, the neoplastic nature of such mediastinal nodes should be confirmed by means of mediastinoscopy or even thoracotomy, aiming at making the correct decision regarding the treatment of patients with non-small cell lung cancer.

Décio Valente, Renck; Daniel Brito de, Araújo; Nilton Haertel, Gomes; Rodrigo, Mendonça.

120

Genetic variation in Australian spotted fever group rickettsiae.  

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Rickettsiae were isolated by cell culture of buffy coat blood from six patients with spotted fever from southeastern Australia and Flinders Island in Bass Strait. The isolates were genetically compared with two previous Rickettsia australis patient isolates. The genus-specific 17-kDA genes from the isolates were compared after DNA amplification and restriction fragment analysis of the amplified DNA. This comparison revealed that mainland rickettsial isolates from southeastern Australia were i...

Baird, R. W.; Stenos, J.; Stewart, R.; Hudson, B.; Lloyd, M.; Aiuto, S.; Dwyer, B.

1996-01-01

 
 
 
 
121

Acquisition of thymidylate by the obligate intracytoplasmic bacterium Rickettsia prowazekii.  

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The pathway for the acquisition of thymidylate in the obligate bacterial parasite Rickettsia prowazekii was determined. R. prowazekii growing in host cells with or without thymidine kinase failed to incorporate into its DNA the [3H]thymidine added to the culture. In the thymidine kinase-negative host cells, the label available to the rickettsiae in the host cell cytoplasm would have been thymidine, and in the thymidine kinase-positive host cells, it would have been both thymidine and TMP. Fur...

Speed, R. R.; Winkler, H. H.

1991-01-01

122

Intraocular inflammation as the main manifestation of Rickettsia conorii infection  

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Full Text Available Archimedes LD Agahan1,3, Jenice Torres1, Graciana Fuentes-Páez1, Hernan Martínez-Osorio1, Antonio Orduña2, Margarita Calonge11Ocular Immunology and Uveitis Unit, Institute of Applied Ophthalmobiology (IOBA, 2Microbiology Department, University of Valladolid, Valladolid, Spain; 3Department of Ophthalmology and Visual Sciences, University of the Philippines, Philippine General Hospital, Manila, PhilippinesObjective: To report the clinical features and management of seven cases of intraocular inflammation caused by Rickettsia infection and review published literature.Methods: Rickettsia conorii or Rickettsia spp. infection was diagnosed based on the following criteria: (1 positive serology according to the European Guidelines, (2 titer normalization after specific treatment, and (3 complete resolution of ophthalmic disease and accompanying symptoms after antibiotic therapy.Results: Seven patients were referred for uveitis of unknown etiology. All came from regions where Mediterranean spotted fever is prevalent. One patient met the European guidelines criteria for Rickettsia spp. infection, while the other six cases met the criteria for R. conorii infection. The main symptoms were visual loss, floaters, eye redness, photophobia, and ocular pain. Predominant ophthalmic signs included vasculitis, choroiditis, vitritis, and macular edema. All patients required antibiotic treatment that resulted in the remission of the infection. Doxycycline was the first choice and the only antibiotic used to treat four patients. One patient needed ciprofloxacin as a second antibiotic after not responding to doxycycline. Two patients had doxycycline as a second antibiotic after not responding primarily to sulfonamides (which had been given after 2–3 days of doxycycline gastric intolerance; one of these patients needed ciprofloxacin as a third antibiotic.Conclusion: Intraocular inflammation can occur as the main manifestation of Rickettsia conorii or Rickettsia spp. infection. It should be considered as a differential diagnosis for uveitis especially for patients living in countries where this infection is endemic in the world. Antibiotic treatment remains effective in the management of Rickettsia infection.Keywords: intraocular inflammation, Mediterranean spotted fever, Rickettsia conorii, uveitis

Orduña A

2011-09-01

123

Human endothelial cell culture plaques induced by Rickettsia rickettsii.  

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Primary cultures of human umbilical vein endothelial cells were inoculated with plaque-purified Rickettsia rickettsii. After adsorption of rickettsiae, monolayers were overlaid with medium containing 0.5% agarose. Small plaques appeared on day 4 postinoculation, and distinct 1- to 2-mm plaques were observed on day 5. Plaquing efficiency was less than that of primary chicken embryo cells in the same medium. Human endothelial cell monolayers were susceptible to infection by R. rickettsii and un...

Walker, D. H.; Firth, W. T.; Edgell, C. J.

1982-01-01

124

Detection of Astrakhan fever rickettsia from ticks in Kosovo.  

Science.gov (United States)

Astrakhan fever is a summer spotted fever resembling Mediterranean spotted fever, endemic in Astrakhan, a region of Russia located by the Caspian sea. Its agent is a spotted fever group rickettsia, member of the Rickettsia conorii complex, transmitted to humans by Rhipicephalus sanguineus and Rhipicephalus pumilio ticks. In Summer 2001, French United Nations troops in Kosovo collected 2 ticks on asymptomatic soldiers (1 R. sanguineus and 1 Hyalomma marginatum) and 10 ticks on dogs (7 R. sanguineus, 2 Ixodes ricinus, and 1 H. marginatum) in the Morina region. By PCR amplification of both the gltA and ompA genes, we detected a rickettsia in 4 R. sanguineus, i.e., 3 of those collected on dogs and those taken from military personnel. As ticks were preserved in alcohol, culture was not possible. The sequences obtained from these PCR products identified, with a 100% homology, Astrakhan fever rickettsia. None of the other collected tick species was positive. The patient with the positive tick remained asymptomatic. Our study demonstrates, for the first time, the presence of Astrakhan fever rickettsia in ticks outside Russia. We suspect that the area of distribution of this rickettsia could be wider than initially suspected. Moreover, as R. sanguineus ticks bite humans, Astrakhan fever might be a cause of spotted fever in Kosovo. PMID:12860620

Fournier, Pierre-Edouard; Durand, Jean-Paul; Rolain, Jean-Marc; Camicas, Jean-Louis; Tolou, Hervé; Raoult, Didier

2003-06-01

125

Presence of Rickettsia felis in the Cat Flea from Southwestern Europe1  

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Rickettsia felis, formerly called ELB agent, was identified by using molecular biology techniques in the cat flea (Ctenocephalides felis felis) from southwestern Spain. For the first time this flea-transmitted rickettsia has been detected within its vector in Eurasia.

Ma?rquez, Francisco J.; Muniain, Miguel A.; Pe?rez, Jesu?s M.; Pacho?n, Jero?nimo

2002-01-01

126

Genome Sequence of Rickettsia conorii subsp. israelensis, the Agent of Israeli Spotted Fever  

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Rickettsia conorii subsp. israelensis is the agent of Israeli spotted fever. The present study reports the draft genome of Rickettsia conorii subsp. israelensis strain ISTT CDC1, isolated from a Rhipicephalus sanguineus tick collected in Israel.

Sentausa, Erwin; El Karkouri, Khalid; Robert, Catherine; Raoult, Didier; Fournier, Pierre-edouard

2012-01-01

127

Genome Sequence of Rickettsia conorii subsp. caspia, the Agent of Astrakhan Fever  

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Rickettsia conorii subsp. caspia is the agent of Astrakhan fever, a spotted fever group rickettsiosis endemic to Astrakhan, Russia. The present study reports the draft genome of Rickettsia conorii subsp. caspia strain A-167.

Sentausa, Erwin; El Karkouri, Khalid; Robert, Catherine; Raoult, Didier; Fournier, Pierre-edouard

2012-01-01

128

Rickettsia Symbionts Cause Parthenogenetic Reproduction in the Parasitoid Wasp Pnigalio soemius (Hymenoptera: Eulophidae)?  

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Bacteria in the genus Rickettsia are intracellular symbionts of disparate groups of organisms. Some Rickettsia strains infect vertebrate animals and plants, where they cause diseases, but most strains are vertically inherited symbionts of invertebrates. In insects Rickettsia symbionts are known to have diverse effects on hosts ranging from influencing host fitness to manipulating reproduction. Here we provide evidence that a Rickettsia symbiont causes thelytokous parthenogenesis (in which mot...

Giorgini, M.; Bernardo, U.; Monti, M. M.; Nappo, A. G.; Gebiola, M.

2010-01-01

129

Exotic Rickettsiae in Ixodes ricinus: fact or artifact?  

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Abstract Several pathogenic Rickettsia species can be transmitted via Ixodes ricinus ticks to humans and animals. Surveys of I. ricinus for the presence of Rickettsiae using part of its 16S rRNA gene yield a plethora of new and different Rickettsia sequences. Interpreting these data is sometimes difficult and presenting these findings as new or potentially pathogenic Rickettsiae should be done with caution: a recent report suggested presence of a ...

Tijsse-Klasen Ellen; Fonville Manoj; van Overbeek Leo; Hj, Reimerink Johan; Sprong Hein

2010-01-01

130

Serological evidence of typhus group rickettsia in a homeless population in Houston, Texas.  

Science.gov (United States)

We tested sera from 176 homeless people in Houston for antibodies against typhus group rickettsiae (TGR). Sera from 19 homeless people were reactive to TGR antigens by ELISA and IFA. Two people had antibodies against Rickettsia prowazekii (epidemic typhus) and the remaining 17 had antibodies against Rickettsia typhi (murine typhus). PMID:18697325

Reeves, Will K; Murray, Kristy O; Meyer, Tamra E; Bull, Lara M; Pascua, Rhia F; Holmes, Kelly C; Loftis, Amanda D

2008-06-01

131

Molecular Detection and Identification of Rickettsia Species in Ixodes pacificus in California  

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We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia.

Phan, Jimmy Ninh; Lu, Casey Roy; Bender, William Garrett; Smoak, Robert Marion; Zhong, Jianmin

2011-01-01

132

Rickettsia infection in dogs and Rickettsia parkeri in Amblyomma tigrinum ticks, Cochabamba Department, Bolivia  

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Only few published data are available on ticks and tick-borne zoonotic pathogens in Bolivia. To evaluate rickettsial seroprevalence and infection in dogs and ticks, during February–April 2007, we collected whole blood, sera, and ticks from dogs living in the rural, peri-urban, and urban areas of Cochabamba, Bolivia. Dog sera were subjected to enzyme-linked immunosorbent assay test to detect IgG antibodies against Rickettsia rickettsii and 68.2% of samples were found to be positi...

Tomassone, Laura; Meneghi, Daniele

2010-01-01

133

Serological identification of Rickettsia spp from the spotted fever group in capybaras in the region of Campinas - SP - Brazil Identificação sorológica de Rickettsia spp do grupo da febre maculosa em capivaras na região de Campinas, SP, Brasil  

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Full Text Available Diseases transmitted by ticks have been an important health problem all over the world. Brazilian Spotted Fever (BSF stands for a serious epidemiological concern due to the high mortality rates pointed out. Capybaras are commonly incriminated as possible reservoirs in the BSF transmission cycle. In the last decades the numbers of these animals raised sharply and they have invaded human areas. They intensify the contact between ticks and humans beings. This study aim is to contribute to the possible role performed for this rodent in the BSF epidemiology in some areas located in Campinas region, São Paulo. Cabybaras infected by rickettsiae of BSF group were studied through the analysis of the frequencies of BSF-group rickettisae antibodies titer = 64 by indirect immunofluorescence test (IFA, and data from human cases epidemiological surveillance. The serum frequency positiveness varied greatly according to areas where animals were captured. However it was found serum positiviness only in the areas where human cases of BSF were reported. These findings suggest the capybara may be seen as sentinel animal. Due to presence of serological cross reactivity between microorganisms belonging to SF group, the results must be interpreted carefully and additional methods to distinguish pathogenic rickettsiae are required in our country.Doenças transmitidas por carrapatos vêm sendo um importante problema de saúde pública no mundo. A Febre Maculosa Brasileira (FMB representa um sério risco epidemiológico devido às altas taxas de letalidade apresentadas. As capivaras são freqüentemente incriminadas como possíveis reservatórios no ciclo de transmissão da FMB. Nas últimas décadas o número desses animais cresceu intensamente e eles invadiram os espaços humanos. As capivaras intensificam o contato entre carrapatos e seres humanos na medida em que se apresentam muito infestadas por estes parasitos. O objetivo deste estudo é contribuir para o conhecimento do possível papel desempenhado por este roedor na epidemiologia da FMB em algumas áreas da região de Campinas, SP. Foi estudada a infecção das capivaras por rickettsias do grupo da FMB, por meio da análise das freqüências de anticorpos contra este grupo, nestes animais, e dados da vigilância epidemiológica de casos humanos. A freqüência desses anticorpos variou amplamente entre as localidades, entretanto, só foram encontrados soros com anticorpos com titulagem =64 naquelas onde havia notificação de casos humanos. Estes achados sugerem que a capivara poderá ser um animal sentinela. No entanto, devido à ocorrência de reação cruzada entre os microorganismos do grupo de FM estes resultados devem ser interpretados com cautela e são necessários métodos capazes de distinguir rickettsias patogênicas.

Celso Eduardo de Souza

2008-09-01

134

Serological identification of Rickettsia spp from the spotted fever group in capybaras in the region of Campinas - SP - Brazil / Identificação sorológica de Rickettsia spp do grupo da febre maculosa em capivaras na região de Campinas, SP, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Doenças transmitidas por carrapatos vêm sendo um importante problema de saúde pública no mundo. A Febre Maculosa Brasileira (FMB) representa um sério risco epidemiológico devido às altas taxas de letalidade apresentadas. As capivaras são freqüentemente incriminadas como possíveis reservatórios no ci [...] clo de transmissão da FMB. Nas últimas décadas o número desses animais cresceu intensamente e eles invadiram os espaços humanos. As capivaras intensificam o contato entre carrapatos e seres humanos na medida em que se apresentam muito infestadas por estes parasitos. O objetivo deste estudo é contribuir para o conhecimento do possível papel desempenhado por este roedor na epidemiologia da FMB em algumas áreas da região de Campinas, SP. Foi estudada a infecção das capivaras por rickettsias do grupo da FMB, por meio da análise das freqüências de anticorpos contra este grupo, nestes animais, e dados da vigilância epidemiológica de casos humanos. A freqüência desses anticorpos variou amplamente entre as localidades, entretanto, só foram encontrados soros com anticorpos com titulagem =64 naquelas onde havia notificação de casos humanos. Estes achados sugerem que a capivara poderá ser um animal sentinela. No entanto, devido à ocorrência de reação cruzada entre os microorganismos do grupo de FM estes resultados devem ser interpretados com cautela e são necessários métodos capazes de distinguir rickettsias patogênicas. Abstract in english Diseases transmitted by ticks have been an important health problem all over the world. Brazilian Spotted Fever (BSF) stands for a serious epidemiological concern due to the high mortality rates pointed out. Capybaras are commonly incriminated as possible reservoirs in the BSF transmission cycle. In [...] the last decades the numbers of these animals raised sharply and they have invaded human areas. They intensify the contact between ticks and humans beings. This study aim is to contribute to the possible role performed for this rodent in the BSF epidemiology in some areas located in Campinas region, São Paulo. Cabybaras infected by rickettsiae of BSF group were studied through the analysis of the frequencies of BSF-group rickettisae antibodies titer = 64 by indirect immunofluorescence test (IFA), and data from human cases epidemiological surveillance. The serum frequency positiveness varied greatly according to areas where animals were captured. However it was found serum positiviness only in the areas where human cases of BSF were reported. These findings suggest the capybara may be seen as sentinel animal. Due to presence of serological cross reactivity between microorganisms belonging to SF group, the results must be interpreted carefully and additional methods to distinguish pathogenic rickettsiae are required in our country.

Celso Eduardo de, Souza; Savina Silvana Lacerra de, Souza; Virgília Luna Castor, Lima; Simone Berger, Calic; Maria Cecilia Gibrail Oliveira, Camargo; Elisa San Martin Mouriz, Savani; Sandra Regina Nicoletti, D' Auria; Arício Xavier, Linhares; Natalino Hajime, Yoshinari.

135

Genotypic characterization of Rickettsiae by DNA probes generated from Rickettsia Prowazekii DNA  

International Nuclear Information System (INIS)

Southern blot analysis of HindIII-cleaved rickettsial DNA was used for genotypic characterization of the typhus group (TG) species (R. prowazekii, R. typhi, R. canada) and a few species were of the spotted fever group (SFG)rickettsiae (R. sibirica, R. conorii, R. akari). Four different DNA probes were employed. PBH11 and PBH13 probes were morphospecific HindIII fragment of R prowazekii DNA. MW218 probe contained the gene for 51 K antigen and MW264 probe contained the citrate synthase gene of R. prowazekii. All the probes hybridized with the tested TG and SFG rickettsial DNAs, forming from 1 to 5 bands, but they did not with R. tsutsudamushi or C. burnetii DNAs. All the probes demonstrated specific hybridization pattern with TG species and R. akari. PBH11. PBH13 and MW264 probes clearly distinguished R. sibirica and R. conorii from the other tested rickettsiae, but not from each other. However, these two species differed slightly with MW218 probe. Several strains of each species were analyzed in this way and except for strains of R. conorii identical intra-species pattern were obtained. These data lead us to consider the obtained hybridization patterns as criteria for genotypic identification. (author)

1994-04-01

136

Rickettsia conorii israelensis in Rhipicephalus sanguineus ticks, Sardinia, Italy.  

Science.gov (United States)

The presence of tick-borne Rickettsia spp. was examined by PCR using DNA samples extracted from 254 ticks collected from mammals originating from northern and eastern Sardinia, Italy. The spotted fever group rickettsial agent Rickettsia conorii israelensis was detected in 3 Rhipicephalus sanguineus ticks from a dog for the first time in this geographical area. In addition, Ri. massiliae, Ri. slovaca, and Ri. aeschlimannii were detected in Rh. turanicus, Rh. sanguineus, Dermacentor marginatus, and Hyalomma marginatum marginatum ticks from dogs, goats, wild boar, and horse. Moreover, Candidatus Rickettsia barbariae was detected in 2 Rh. turanicus ticks from goats. The detection of Ri. conorii israelensis, an emergent agent which causes Israeli spotted fever, increases our knowledge on tick-borne rickettsioses in Sardinia. PMID:24852264

Chisu, Valentina; Masala, Giovanna; Foxi, Cipriano; Socolovschi, Cristina; Raoult, Didier; Parola, Philippe

2014-06-01

137

Partial purification and characterization of the major species-specific protein antigens of Rickettsia typhi and Rickettsia prowazekii identified by rocket immunoelectrophoresis.  

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Species-specific antigens from Rickettsia typhi and Rickettsia prowazekii were readily solubilized by French pressure cell extraction or sonication of Renografin density gradient-purified rickettsiae and were identified by rocket immunoelectrophoresis. As measured by quantitative rocket immunoelectrophoresis, the species-specific typhus rocket antigens (STRAs) appeared to be proteins; they were denatured by heating at 56 degrees C for 30 min but not by 50 degrees C treatment, and they were se...

Dasch, G. A.; Samms, J. R.; Williams, J. C.

1981-01-01

138

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil Soroprevalência de Rickettsia bellii e Rickettsia felis em cães, São José dos Pinhais, Paraná, Brasil  

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Brazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirec...

Fernanda Silva Fortes; Iara Silveira; Jonas Moraes-Filho; Ronaldo Viana Leite; José Edivaldo Bonacim; Alexander Welker Biondo; Marcelo Bahia Labruna; Marcelo Beltrão Molento

2010-01-01

139

New Rickettsia sp. in tsetse flies from Senegal.  

Science.gov (United States)

Tsetse flies are blood-sucking insects transmitting African trypanosomiasis. They are known to harbor also three intracellular bacteria that play important role in their lifecycle: Wigglesworthia glossinidia, Sodalis glossinidius and Wolbachia sp. We have studied 78 Glossina morsitans submorsitans collected in Senegal. In all studied flies we amplified genes of bacterium phylogenetically close to obligate intracellular pathogen Rickettsia felis, the agent of spotted fever in humans. We also visualized this rickettsia in the cells of tsetse flies by fluorescence in situ hybridization. The role of this probable fourth endosymbiotic bacterium of tsetse flies in Glossina lifecycle and possible pathogenecity for humans should be further investigated. PMID:22277830

Mediannikov, O; Audoly, G; Diatta, G; Trape, J-F; Raoult, D

2012-03-01

140

Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species  

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Full Text Available Abstract Background Hard ticks have been identified as important vectors of rickettsiae causing the spotted fever syndrome. Tick-borne rickettsiae are considered to be emerging, but only limited data are available about their presence in Western Europe, their natural life cycle and their reservoir hosts. Ixodes ricinus, the most prevalent tick species, were collected and tested from different vegetation types and from potential reservoir hosts. In one biotope area, the annual and seasonal variability of rickettsiae infections of the different tick stages were determined for 9 years. Results The DNA of the human pathogen R. conorii as well as R. helvetica, R. sp. IRS and R. bellii-like were found. Unexpectedly, the DNA of the highly pathogenic R. typhi and R. prowazekii and 4 other uncharacterized Rickettsia spp. related to the typhus group were also detected in I. ricinus. The presence of R. helvetica in fleas isolated from small rodents supported our hypothesis that cross-infection can occur under natural conditions, since R. typhi/prowazekii and R. helvetica as well as their vectors share rodents as reservoir hosts. In one biotope, the infection rate with R. helvetica was ~66% for 9 years, and was comparable between larvae, nymphs, and adults. Larvae caught by flagging generally have not yet taken a blood meal from a vertebrate host. The simplest explanation for the comparable prevalence of R. helvetica between the defined tick stages is, that R. helvetica is vertically transmitted through the next generation with high efficiency. The DNA of R. helvetica was also present in whole blood from mice, deer and wild boar. Conclusion Besides R. helvetica, unexpected rickettsiae are found in I. ricinus ticks. We propose that I. ricinus is a major reservoir host for R. helvetica, and that vertebrate hosts play important roles in the further geographical dispersion of rickettsiae.

Gaasenbeek Cor

2009-09-01

 
 
 
 
141

Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species  

DEFF Research Database (Denmark)

ABSTRACT: BACKGROUND: Hard ticks have been identified as important vectors of rickettsiae causing the spotted fever syndrome. Tick-borne rickettsiae are considered to be emerging, but only limited data are available about their presence in Western Europe, their natural life cycle and their reservoir hosts. Ixodes ricinus, the most prevalent tick species, were collected and tested from different vegetation types and from potential reservoir hosts. In one biotope area, the annual and seasonal variability of rickettsiae infections of the different tick stages were determined for 9 years. RESULTS: The DNA of the human pathogen R. conorii as well as R. helvetica, R. sp. IRS and R. bellii-like were found. Unexpectedly, the DNA of the highly pathogenic R. typhi and R. prowazekii and 4 other uncharacterized Rickettsia spp. related to the typhus group were also detected in I. ricinus. The presence of R. helvetica in fleas isolated from small rodents supported our hypothesis that cross-infection can occur under naturalconditions, since R. typhi/prowazekii and R. helvetica as well as their vectors share rodents as reservoir hosts. In one biotope, the infection rate with R. helvetica was ~66% for 9 years, and was comparable between larvae, nymphs, and adults. Larvae caught by flagging generally have not yet taken a blood meal from a vertebrate host. The simplest explanation for the comparable prevalence of R. helvetica between the defined tick stages is, that R. helvetica is vertically transmitted through the next generation with high efficiency. The DNA of R. helvetica was also present in whole blood from mice, deer and wild boar. CONCLUSION: Besides R. helvetica, unexpected rickettsiae are found in I. ricinus ticks. We propose that I. ricinus is a major reservoir host for R. helvetica, and that vertebrate hosts play important roles in the further geographical dispersion of rickettsiae.

Sprong, Hein; Wielinga, Pieter

2009-01-01

142

Isolation of a Spotted Fever Group Rickettsia, Rickettsia peacockii, in a Rocky Mountain Wood Tick, Dermacentor andersoni, Cell Line  

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An embryonic cell line (DAE100) of the Rocky Mountain wood tick, Dermacentor andersoni, was observed by microscopy to be chronically infected with a rickettsialike organism. The organism was identified as a spotted fever group (SFG) rickettsia by PCR amplification and sequencing of portions of the 16S rRNA, citrate synthase, Rickettsia genus-specific 17-kDa antigen, and SFG-specific 190-kDa outer membrane protein A (rOmpA) genes. Sequence analysis of a partial rompA gene PCR fragment and indi...

Simser, Jason A.; Palmer, Ann T.; Munderloh, Ulrike G.; Kurtti, Timothy J.

2001-01-01

143

A ocorrência de riquetsioses do grupo Rickettsia rickettsii Occurrence of rickettsiosis of the group Rickettsia rickettsii  

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Full Text Available Foi realizada revisão da literatura com objetivo de atualizar as informações sobre a ocorrência de riquetsioses do grupo Rickettsia rickettsii. Verificou-se que nos EUA e Europa, a incidência da febre maculosa, vem aumentando desde 1970 até hoje. No Brasil, foi relatado um caso presuntivo, no estado da Bahia, em 1979. Com relação a prevenção, controle e tratamento dessa doença é salientada a importância de informações relacionadas com indivíduos expostos a picadas de carrapatos, notificação de novos casos, fatores ecológicos, técnicas laboratoriais mais específicas para a identificação do agente etiológico, e a antibioticoterapia mais eficiente. A vacinação é ainda referida como meio mais favorável na prevenção da doença, devendo ser administrada aos indivíduos de alto risco. No Brasil, faltam informações precisas sobre a ocorrência de R. rickettsii.A search of the literature to update the available information on the occurrence of rickettsiosis caused by the Rickettsia rickettsii group was made. It was verified that the incidence of spotted fever has had an increase in the U.S.A. and Europe since 1970. In Brazil, a presumptive case was reported in the State of Bahia, in 1979. Regarding the prevention, control and treatment of this disease, importance is given to data related to individuals exposed to tick bites, report of new cases, ecological factors, more specific laboratorial procedures for the identification of the etiological agent, and a more efficient antibiotic therapy. Vaccination is still regarded as the most adequate means for the prevention of the disease, and should be aimed at groups of individuals at high risk. In Brazil, there is a lack of more precise information on the occurrence of R. rickettsii.

Dalva A. Portari Mancini

1983-12-01

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Flying squirrel-associated Rickettsia prowazekii (epidemic typhus rickettsiae) characterized by a specific DNA fragment produced by restriction endonuclease digestion.  

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The DNA from flying squirrel-associated Rickettsia prowazekii was characterized by using a specific DNA fragment produced by digestion with the enzyme BamHI. The DNA fragment was cloned into a plasmid vector and used to readily distinguish between available human- and flying squirrel-associated R. prowazekii DNAs derived from crude cytoplasmic extracts.

Regnery, R. L.; Fu, Z. Y.; Spruill, C. L.

1986-01-01

145

Coinfections of Rickettsia slovaca and Rickettsia helvetica with Borrelia lusitaniae in ticks collected in a Safari Park, Portugal.  

Science.gov (United States)

Borrelia and Rickettsia bacteria are the most important tick-borne agents causing disease in Portugal. Identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. A total of 677 questing ticks including Dermacentor marginatus, Rhipicephalus sanguineus, Ixodes ricinus, Hyalomma lusitanicum, H. marginatum, and Haemaphysalis punctata were collected in a Safari Park in Alentejo, Portugal, to investigate the prevalences of infection and characterize Borrelia and Rickettsia species. From a total of 371 ticks tested by PCR for Borrelia burgdorferi sensu lato (s.l.), of which 247 were tested for Rickettsia, an infection prevalence of 18.3% was found for B. lusitaniae and 55.1% for Rickettsia spp. Sequence analysis of positive amplicons identified the presence of B. lusitaniae (18.3%), R. monacensis strain IRS3 (51.7%), and R. helvetica (48.3%) in I. ricinus. R. slovaca (41.5%), R. raoultii (58.5%), and also B. lusitaniae (21%) were identified in D. marginatus ticks. One (5.9%) H. lusitanicum was infected with B. lusitaniae, and R. massiliae was found in one Rhipicephalus sanguineus. Coinfection was found in 7 (20%) I. ricinus and 34 (23.3%) D. marginatus ticks. We report, for the first time, simultaneous infection with R. helvetica and B. lusitaniae and also R. slovaca, the agent of TIBOLA/DEBONEL, with B. lusitaniae. Additionally, 6 isolates of B. lusitaniae were established, and isolates of Rickettsia were also obtained for the detected species using tick macerates cultured in mammalian and mosquito cell lines. This report describes the detection and isolation of tick-borne agents from a Portuguese Safari Park, highlighting the increased likelihood of infection with multiple agents to potential visitors or staff. PMID:21771525

Milhano, Natacha; de Carvalho, Isabel Lopes; Alves, Ana Sofia; Arroube, Sofia; Soares, Jorge; Rodriguez, Pablo; Carolino, Manuela; Núncio, Maria Sofia; Piesman, Joseph; de Sousa, Rita

2010-12-01

146

Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain.  

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Ticks recovered from dogs in rural villages around Madrid (Spain) were processed to isolate rickettsiae. One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to Rickettsia conorii, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultur...

Herrero, C.; Pelaz, C.; Marti?n-bourgon, C.

1992-01-01

147

Ultrastructure of Rickettsia rickettsii Actin Tails and Localization of Cytoskeletal Proteins  

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Actin-based motility (ABM) is a mechanism for intercellular spread that is utilized by vaccinia virus and the invasive bacteria within the genera Rickettsia, Listeria, and Shigella. Within the Rickettsia, ABM is confined to members of the spotted fever group (SFG), such as Rickettsia rickettsii, the agent of Rocky Mountain spotted fever. Infection by each agent induces the polymerization of host cell actin to form the typical F (filamentous)-actin comet tail. Assembly of the actin tail propel...

Kirk, Levi S.; Hayes, Stanley F.; Heinzen, Robert A.

2000-01-01

148

Expression of an Epitope-Tagged Virulence Protein in Rickettsia parkeri Using Transposon Insertion  

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Despite recent advances in our ability to genetically manipulate Rickettsia, little has been done to employ genetic tools to study the expression and localization of Rickettsia virulence proteins. Using a mariner-based Himar1 transposition system, we expressed an epitope-tagged variant of the actin polymerizing protein RickA under the control of its native promoter in Rickettsia parkeri, allowing the detection of RickA using commercially-available antibodies. Native RickA and epitope-tagged R...

Welch, Matthew D.; Reed, Shawna C. O.; Lamason, Rebecca L.; Serio, Alisa W.

2012-01-01

149

In vitro susceptibilities of spotted fever group rickettsiae and Coxiella burnetti to clarithromycin.  

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The in vitro bacteriostatic activity of clarithromycin, a new macrolide derivative, against Rickettsia rickettsii, Rickettsia conorii, and "Rickettsia israeli" was determined by the plaque assay and the dye uptake assay. Both bacteriostatic and bactericidal activities of clarithromycin against the Nine Mile, Q212, Priscilla, and ME9 strains of Coxiella burnetti were evaluated by using three cell culture systems. Clarithromycin showed improved antibacterial activity compared with that ...

1993-01-01

150

Structural Properties of Lipopolysaccharides from Rickettsia typhi and Rickettsia prowazekii and Their Chemical Similarity to the Lipopolysaccharide from Proteus vulgaris OX19 Used in the Weil-Felix Test  

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The lipopolysaccharides (LPSs) isolated from typhus group (TG) rickettsiae Rickettsia typhi and Rickettsia prowazekii were characterized by chemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining. LPSs from two species of TG rickettsiae contained glucose, 3-deoxy-d-manno-octulosonic acid, glucosamine, quinovosamine, phosphate, and fatty acids (?-hydroxylmyristic acid and heneicosanoic acid) but not heptose. The O-polysaccharides ...

1998-01-01

151

Detection of ‘‘Candidatus Rickettsia sp. strain Argentina’’and R. bellii in Amblyomma ticks (Acari: Ixodidae) from Northern Argentina  

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Ixodid ticks were collected from vegetation and from humans, wild and domestic mammals in a rural area in the semi-arid Argentine Chaco in late spring 2006 to evaluate their potential role as vectors of Spotted Fever Group (SFG) rickettsiae. A total of 233 adult ticks, identified as Amblyomma parvum, Amblyomma tigrinum and Amblyomma pseudoconcolor, was examined for Rickettsia spp. We identified an SFG rickettsia of unknown pathogenicity, ‘‘Candidatus Rickettsia sp. strain Argentina’’,...

Tomassone, Laura

2010-01-01

152

Anti-Rickettsia spp. antibodies in free-ranging and captive capybaras from southern Brazil Anticorpos anti-Rickettsia spp. em capivaras de vida livre e de cativeiro no Sul do Brasil  

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Full Text Available Capybaras (Hydrochaeris hydrochaeris are among the main hosts of Amblyomma spp. ticks, which is able to transmit Rickettsia species to human beings and animals. Since they are often infested with potential vector ticks, capybaras may be used as sentinels for rickettsiosis, such as the Brazilian Spotted Fever. The aim of the present study was to determine the prevalence of antibodies against Rickettsia spp. using the indirect immunofluorescence assay (IFA in 21 free-ranging and 10 captive animals from the Zoological Park of the 'Bela Vista Biological Sanctuary' (BVBS, Itaipu Binational, Foz do Iguaçu, Southern Brazil. Antigens of six rickettsial species already identified in Brazil (Rickettsia rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis were used for IFA. Ticks from each capybara were collected for posterior taxonomic identification. A total of 19 (61.3% samples reacted to at least one of tested species. Seropositivity was found in 14 (45.2%, 12 (38.7%, 5 (16.1%, 4 (12.9%, 3 (9.7% and 3 (9.7% animals for R. rickettsii, R. bellii, R. parkeri, R. amblyommii, R. felis and R. rhipicephali, respectively. Two captive capybaras presented suggestive titers of R. rickettsii infection and one sample showed homologous reaction to R. parkeri. Only one free-ranging capybara presented evidence R. bellii infection. Ticks collected on capybaras were identified as Amblyomma dubitatum e Amblyomma sp. Results evidenced the rickettsial circulation in the area, suggesting a potential role of capybaras on bacterial life cycle.As capivaras (Hydrochaeris hydrochaeris estão entre os principais hospedeiros do carrapato Amblyomma spp., o qual pode transmitir algumas espécies de riquétsias para seres humanos e animais. Como são frequentemente infestadas por carrapatos vetores potenciais, as capivaras podem ser usadas como sentinelas para riquetsioses, como a Febre Maculosa Brasileira. O objetivo do presente estudo foi determinar a soroprevalência de Rickettsia spp. por meio da reação de Imunofluorescência Indireta (RIFI em 21 capivaras de vida livre e 10 capivaras de cativeiro do Zoológico do Refúgio Biológico Bela Vista, Itaipu Binacional, Foz do Iguaçu, Brasil. Antígenos de seis espécies de riquétsias já identi[1]icadas no Brasil (Rickettsia rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis foram utilizados para a RIFI. Carrapatos de cada capivara foram coletados para posterior identi[1]icação taxonômica. Um total de 19 (61,3% amostras reagiu a pelo menos uma das espécies testadas. Foi encontrada soropositividade em 14 (45,2%, 12 (38,7%, 5 (16,1%, 4 (12,9%, 3 (9,7% e 3 (9,7% animais para R. rickettsii, R. bellii, R. parkeri, R. amblyommii, R. felis e R. rhipicephali, respectivamente. Duas capivaras de cativeiro apresentaram títulos sugestivos de infecção por R. rickettsii e uma amostra apresentou reação homóloga frente à R. parkeri. Apenas uma capivara de vida livre apresentou evidência de infecção por R. bellii. Os carrapatos coletados sobre as capivaras foram identificados como Amblyomma dubitatum e Amblyomma sp. Os resultados evidenciam a circulação de riquétsias na região, sugerindo uma potencial participação da capivara no ciclo de vida desta bactéria.

Fernanda S. Fortes

2011-11-01

153

Anti-Rickettsia spp. antibodies in free-ranging and captive capybaras from southern Brazil / Anticorpos anti-Rickettsia spp. em capivaras de vida livre e de cativeiro no Sul do Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese As capivaras (Hydrochaeris hydrochaeris) estão entre os principais hospedeiros do carrapato Amblyomma spp., o qual pode transmitir algumas espécies de riquétsias para seres humanos e animais. Como são frequentemente infestadas por carrapatos vetores potenciais, as capivaras podem ser usadas como sen [...] tinelas para riquetsioses, como a Febre Maculosa Brasileira. O objetivo do presente estudo foi determinar a soroprevalência de Rickettsia spp. por meio da reação de Imunofluorescência Indireta (RIFI) em 21 capivaras de vida livre e 10 capivaras de cativeiro do Zoológico do Refúgio Biológico Bela Vista, Itaipu Binacional, Foz do Iguaçu, Brasil. Antígenos de seis espécies de riquétsias já identi[1]icadas no Brasil (Rickettsia rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis) foram utilizados para a RIFI. Carrapatos de cada capivara foram coletados para posterior identi[1]icação taxonômica. Um total de 19 (61,3%) amostras reagiu a pelo menos uma das espécies testadas. Foi encontrada soropositividade em 14 (45,2%), 12 (38,7%), 5 (16,1%), 4 (12,9%), 3 (9,7%) e 3 (9,7%) animais para R. rickettsii, R. bellii, R. parkeri, R. amblyommii, R. felis e R. rhipicephali, respectivamente. Duas capivaras de cativeiro apresentaram títulos sugestivos de infecção por R. rickettsii e uma amostra apresentou reação homóloga frente à R. parkeri. Apenas uma capivara de vida livre apresentou evidência de infecção por R. bellii. Os carrapatos coletados sobre as capivaras foram identificados como Amblyomma dubitatum e Amblyomma sp. Os resultados evidenciam a circulação de riquétsias na região, sugerindo uma potencial participação da capivara no ciclo de vida desta bactéria. Abstract in english Capybaras (Hydrochaeris hydrochaeris) are among the main hosts of Amblyomma spp. ticks, which is able to transmit Rickettsia species to human beings and animals. Since they are often infested with potential vector ticks, capybaras may be used as sentinels for rickettsiosis, such as the Brazilian Spo [...] tted Fever. The aim of the present study was to determine the prevalence of antibodies against Rickettsia spp. using the indirect immunofluorescence assay (IFA) in 21 free-ranging and 10 captive animals from the Zoological Park of the 'Bela Vista Biological Sanctuary' (BVBS), Itaipu Binational, Foz do Iguaçu, Southern Brazil. Antigens of six rickettsial species already identified in Brazil (Rickettsia rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis) were used for IFA. Ticks from each capybara were collected for posterior taxonomic identification. A total of 19 (61.3%) samples reacted to at least one of tested species. Seropositivity was found in 14 (45.2%), 12 (38.7%), 5 (16.1%), 4 (12.9%), 3 (9.7%) and 3 (9.7%) animals for R. rickettsii, R. bellii, R. parkeri, R. amblyommii, R. felis and R. rhipicephali, respectively. Two captive capybaras presented suggestive titers of R. rickettsii infection and one sample showed homologous reaction to R. parkeri. Only one free-ranging capybara presented evidence R. bellii infection. Ticks collected on capybaras were identified as Amblyomma dubitatum e Amblyomma sp. Results evidenced the rickettsial circulation in the area, suggesting a potential role of capybaras on bacterial life cycle.

Fernanda S., Fortes; Leonilda C., Santos; Zalmir S., Cubas; Ivan R., Barros-Filho; Alexander W., Biondo; Iara, Silveira; Marcelo B., Labruna; Marcelo B., Molento.

154

Effects of dorsal root ganglion destruction by adriamycin in patients with postherpetic neuralgia / Efeitos da destruição da raiz dorsal ganglionar pela adriamicina em pacientes com neuralgia pós-herpética  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese OBJETIVO: Investigar os efeitos da destruição da raiz dorsal ganglionar em pacientes com neuralgia pós-herpética. MÉTODOS: Setenta e dois pacientes selecionados com neuralgia pós-herpética foram randomicamente distribuídos em dois grupos (n=36). Grupo A foi o grupo controle (tratado por injeção) e o [...] grupo B foi o grupo com destruição da raiz dorsal do gânglio pela adriamicina. Os escores da Escala Analógica Visual (VAS), SAS, SF-MPQ escores, efeitos clínicos e segurança terapêutica foram avaliados as antes da terapia, uma semana, três e seis meses após a terapia. Quarenta e quatro pacientes foram avaliados pela análise de intenção-em-tratar. RESULTADOS: A média dos escores de dor na escala de Likert foi significativamente reduzida em cada ponto no grupo B. Pacientes no grupo B relataram efetividade clínica aos seis meses com excelente resposta (16), boa resposta (12), melhora mais insatisfatória ou sem modificações (8). Escores VAS a cada tempo após o procedimento foram melhores em comparação ao pré-operatório. No grupo A não foi observada diferença significativa. Pacientes mostraram melhora nos escores de dormir no grupo B. Houve diferença significante no T2 no grupo A que T1. Não houve diferença significante no grupo A nos tempos T3 e T4 após a cirurgia em relação a antes. Comparação entre os grupos: houve diferença significante entre os grupos A e B a cada tempo após a cirurgia. CONCLUSÕES: A destruição da raiz dorsal ganglionar pela adriamicina sob perspectiva guiada pelo C-arm, a cirurgia pontual foi acurada sem qualquer reação adversa ou complicação séria, que pode efetivamente aliviar a dor em pacientes com neuralgia pós-herpética, mas os efeitos de longo prazo necessitam mais estudos. Abstract in english PURPOSE: To investigate the effects of dorsal root ganglion destruction in patients with postherpetic neuralgia (PHN). METHODS: Seventy-two patients with PHN selected were randomly divided into two groups (n=36). Group A was the control group (treated by injection) and group B was the group of dorsa [...] l root ganglion destruction by adriamycin. Visual analog scale scores (VAS), SAS, SF-MPQ scores. Clinical effects and therapy safety were evaluated before therapy, one week, three and six months after therapy. Forty-four patients were available for intention-to-treat analysis. RESULTS: The average pain scores on the Likert scale were significantly reduced at each point in group B. Patients in group B reported clinical effectiveness at six months as excellent response, good response, improved but unsatisfactory or unchanged 16, 12 and 8.VAS scores at each time point after the operation were lower than that before operation and in group A, there was significant difference. Patients showed significant improvement in sleep scores in group B. There was significant difference at T2 in group A than T1. There was no significant difference in group A at T3, T4 after the operation than that before operation. Between group comparison: there was significant difference between group A and group B at each time point after the operation. CONCLUSIONS: Dorsal root ganglion destruction by adriamycin under guidance of C-arm perspective, the puncture operation was accurate without any adverse reaction or serious complications, which could effectively relieve pain of patients with postherpetic neuralgia, but the long-term effects needed further study.

He, Chun-jing; Luo, yi-ran; Nie, hao-xiong.

155

Effects of dorsal root ganglion destruction by adriamycin in patients with postherpetic neuralgia Efeitos da destruição da raiz dorsal ganglionar pela adriamicina em pacientes com neuralgia pós-herpética  

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Full Text Available PURPOSE: To investigate the effects of dorsal root ganglion destruction in patients with postherpetic neuralgia (PHN. METHODS: Seventy-two patients with PHN selected were randomly divided into two groups (n=36. Group A was the control group (treated by injection and group B was the group of dorsal root ganglion destruction by adriamycin. Visual analog scale scores (VAS, SAS, SF-MPQ scores. Clinical effects and therapy safety were evaluated before therapy, one week, three and six months after therapy. Forty-four patients were available for intention-to-treat analysis. RESULTS: The average pain scores on the Likert scale were significantly reduced at each point in group B. Patients in group B reported clinical effectiveness at six months as excellent response, good response, improved but unsatisfactory or unchanged 16, 12 and 8.VAS scores at each time point after the operation were lower than that before operation and in group A, there was significant difference. Patients showed significant improvement in sleep scores in group B. There was significant difference at T2 in group A than T1. There was no significant difference in group A at T3, T4 after the operation than that before operation. Between group comparison: there was significant difference between group A and group B at each time point after the operation. CONCLUSIONS: Dorsal root ganglion destruction by adriamycin under guidance of C-arm perspective, the puncture operation was accurate without any adverse reaction or serious complications, which could effectively relieve pain of patients with postherpetic neuralgia, but the long-term effects needed further study.OBJETIVO: Investigar os efeitos da destruição da raiz dorsal ganglionar em pacientes com neuralgia pós-herpética. MÉTODOS: Setenta e dois pacientes selecionados com neuralgia pós-herpética foram randomicamente distribuídos em dois grupos (n=36. Grupo A foi o grupo controle (tratado por injeção e o grupo B foi o grupo com destruição da raiz dorsal do gânglio pela adriamicina. Os escores da Escala Analógica Visual (VAS, SAS, SF-MPQ escores, efeitos clínicos e segurança terapêutica foram avaliados as antes da terapia, uma semana, três e seis meses após a terapia. Quarenta e quatro pacientes foram avaliados pela análise de intenção-em-tratar. RESULTADOS: A média dos escores de dor na escala de Likert foi significativamente reduzida em cada ponto no grupo B. Pacientes no grupo B relataram efetividade clínica aos seis meses com excelente resposta (16, boa resposta (12, melhora mais insatisfatória ou sem modificações (8. Escores VAS a cada tempo após o procedimento foram melhores em comparação ao pré-operatório. No grupo A não foi observada diferença significativa. Pacientes mostraram melhora nos escores de dormir no grupo B. Houve diferença significante no T2 no grupo A que T1. Não houve diferença significante no grupo A nos tempos T3 e T4 após a cirurgia em relação a antes. Comparação entre os grupos: houve diferença significante entre os grupos A e B a cada tempo após a cirurgia. CONCLUSÕES: A destruição da raiz dorsal ganglionar pela adriamicina sob perspectiva guiada pelo C-arm, a cirurgia pontual foi acurada sem qualquer reação adversa ou complicação séria, que pode efetivamente aliviar a dor em pacientes com neuralgia pós-herpética, mas os efeitos de longo prazo necessitam mais estudos.

He Chun-jing

2012-06-01

156

Patterns and Processes of Molecular Evolution in Rickettsia  

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Species of the genus Rickettsia are obligate intracellular parasites of the a-proteobacterial subdivision. It has been suggested that obligate intracellular bacteria have evolved from free-living bacteria with much larger genome sizes. Transitions to intracellular growth habitats are normally associated with radical genomic alterations, particularly genome rearrangements and gene losses. This thesis presents a comparative study of evolutionary processes such as gene rearrangements, deletions ...

Amiri, Haleh

2002-01-01

157

An update on the detection and treatment of Rickettsia felis  

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Laya Hun, Adriana TroyoCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa RicaAbstract: Rickettsia felis was described as a human pathogen almost two decades ago, and human infection is currently reported in 18 countries in all continents. The distribution of this species is worldwide, determined by the presence of the main arthropod vector, Ctenocephalides felis (Bouché). The list of symptoms, ...

Hun L; Troyo A

2012-01-01

158

Radioiodination of an outer membrane protein in intact Rickettsia prowazekii.  

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Intact Rickettsia prowazekii was radiolabeled with the glucose oxidase-lactoperoxidase method of iodination. Separation of the rickettsial extract into cytoplasmic, outer and inner membrane fractions demonstrated that the outer membrane was preferentially labeled. Analysis of the polypeptides of these fractions on high-resolution slab polyacrylamide gels showed that most of the 125I was in polypeptide T49, an outer membrane constituent. Additional outer membrane polypeptides were iodinated in...

Smith, D. K.; Winkler, H. H.

1980-01-01

159

An infection of Ruditapes decussatus (Bivalvia) by Rickettsia  

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The authors report the presence of a Rickettsia in Ruditapes decussatus from natural populations of the Algarve (Portugal). Light microscopic shows infections of varying importance in gill tissues. Some enclose small procaryotic colonies (cp) scattered in all lamellae; in others, the colonies are scarce and hypertrophic (100 mu m). These different pictures suggest an amplification process. Digestive diverticulae may also be infected, but to a lesser extent. The ultrastructural features of thi...

Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Comps, Michel; Ruano, Francisco; Grizel, Henri

1987-01-01

160

Rickettsia felis infection in cat fleas Ctenocephalides felis felis  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR [...] products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA) showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5%) out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory, 6 (40.0%) reacted positively to R. felis by IFA. Reactive feline and human sera showed low endpoint titers against R. felis, varying from 64 to 256. With the exception of one human serum, all R. felis-reactive sera were also reactive to Rickettsia rickettsii and/or Rickettsia parkeri antigens at similar titers to R. felis. The single human serum that was reactive solely to R. felis had an endpoint titer of 256, indicating that this person was infected by R. felis.

Horta, Mauricio C.; Scott, Fabio B.; Correia, Thaís R.; Fernandes, Julio I.; Richtzenhain, Leonardo J.; Labruna, Marcelo B..

 
 
 
 
161

Growth of Rickettsia felis in Drosophila melanogaster S2 cells.  

Science.gov (United States)

Rickettsia felis is an obligate, intracellular, Gram-negative bacterium and a member of the transitional group rickettsiae. This bacterium has been shown to grow in vitro in amphibian, tick, and mosquito cell lines. Here, we present data to show the growth of R. felis strain LSU in Drosophila melanogaster S2 cells, an embryonic, hemocytic cell line with phagocytic properties. R. felis LSU was isolated from Ixodes scapularis E6 (ISE6) cells and used to infect S2 cells, grown at 25°C. By 19 days postinfection, the S2 cells were 100% infected with R. felis as determined by Acridine Orange and Diff-Quik staining. A species-specific R. felis qPCR assay was used to demonstrate that the kinetics associated with the S2 cell culture infection involved a lag/adaptation phase, followed by continued growth to 20 days postinfection. Moreover, R. felis organisms were observed in the S2 cells using transmission electron microscopy and a polyclonal antibody against spotted fever rickettsiae. The ability to use D. melanogaster S2 cells for growing rickettsial agents is a useful tool owing to the ease of manipulation of the S2 cultures and the wide-ranging possibility of Drosophila resources available for future studies. PMID:24528095

Luce-Fedrow, Alison; Macaluso, Kevin R; Richards, Allen L

2014-02-01

162

Rickettsia felis infection in cat fleas Ctenocephalides felis felis  

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Full Text Available The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females tested by polymerase chain reaction (PCR were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5% out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory, 6 (40.0% reacted positively to R. felis by IFA. Reactive feline and human sera showed low endpoint titers against R. felis, varying from 64 to 256. With the exception of one human serum, all R. felis-reactive sera were also reactive to Rickettsia rickettsii and/or Rickettsia parkeri antigens at similar titers to R. felis. The single human serum that was reactive solely to R. felis had an endpoint titer of 256, indicating that this person was infected by R. felis.

Mauricio C. Horta

2010-10-01

163

Genotyping, evolution and epidemiological findings of Rickettsia species.  

Science.gov (United States)

Rickettsiae are obligate intracellular bacteria that can cause mild to life-threatening diseases, including epidemic typhus, one of the oldest pernicious diseases of mankind. Clinical awareness of rickettsial diseases and molecular diagnosis have shown that rickettsioses should be viewed as new emerging and reemerging diseases. Rickettsia has been shown to be a large genus with a worldwide distribution, a very diverse host range, including hosts that have no relationship with vertebrate. Genomic studies have demonstrated genome reduction due to gene loss associated with increased pathogenicity and horizontal DNA acquisition according to a sympatric mode of evolution in hosts that contain several organisms. This article presents a review of genotyping techniques and examines the principle of genotype determination in terms of taxonomic strategies and detection methods. This article summarizes the epidemiological and pathological features of Rickettsia and discusses the genomic findings that help the understanding of the evolution of pathogenicity including the deleterious mutations of repair systems and the toxin-antitoxin systems. PMID:24662440

Merhej, Vicky; Angelakis, Emmanouil; Socolovschi, Cristina; Raoult, Didier

2014-07-01

164

Resultados comparativos de la disección ganglionar en cáncer de recto con y sin tratamiento previo del tejido adiposo Comparative results of ganglion dissection in cancer of the rectum with and without prior treatment with adipose tissue  

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Full Text Available Fundamento. El propósito de este estudio es describir los resultados obtenidos mediante dos técnicas de aislamiento de ganglios linfáticos en piezas quirúrgicas de resección anterior por adenocarcinoma de recto. Material y métodos. En una serie de 30 casos consecutivos de pacientes intervenidos por adenocarcinoma de recto hemos realizado una búsqueda de ganglios de forma manual convencional y una segunda tras 24 horas en una solución desengrasante a temperatura ambiente. Resultados. En la primera búsqueda se han aislado 335 ganglios linfáticos con una media que oscila entre 6,46 y 17,58, correspondiendo los valores más bajos a los grupos que habían recibido tratamiento adyuvante previo. En la segunda inclusión, tras la acción de la solución de aclaramiento hemos encontrado nuevos ganglios (85 en un 70% de los casos, en número y tamaño sensiblemente inferior al inicial. Conclusiones. La disección ganglionar manual del tejido adiposo es un método fiable para el aislamiento de ganglios linfáticos en las piezas de resección por adenocarcinoma de recto. La búsqueda de ganglios linfáticos tras la acción de una solución de aclaramiento debe reservarse para los casos en los que no se alcanza el mínimo aconsejado en el estadiaje TNM.Background. The aim of this study is to describe the result obtained through two techniques of isolation of lymphatic lymph nodes in surgical pieces of anterior resection due to adenocarcinoma of the rectum. Material and methods. We carried out a search in a series of 30 consecutive cases of patients operated on for adenocarcinoma of the rectum for lymph nodes first in a manual conventional way and second after 24 hours in a degreasing solution at room temperature. Results. In the first search 335 lymph nodes were lymph nodes isolated, with an average that oscillated between 6.46 and 17.58, with the lower values corresponding to the groups that had received prior adjuvant treatment. In the second inclusion, following the action of the clearing solution, we found new lymph nodes (85 in some 75% of the cases, appreciably lower in number and smaller in size than the initial search. Conclusions. Manual lymph nodes dissection of the adipose tissue is a reliable method for the isolation of lymphatic lymph nodes in pieces of resection due to adenocarcinoma of the rectum. The search for lymphatic ganglions following the action of a clearing solution should be reserved for cases in which the minimum recommended in the TNM staging is not reached.

I. Amat

2003-12-01

165

Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil Identificação sorológica de Rickettsia sp. em equinos e cães de área não endêmica no Brasil  

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Full Text Available Brazilian Spotted Fever (BSF is a lethal rickettsiosis in humans caused by the bacteria Rickettsia rickettsii, and is endemic in some areas of Brazil. Horses and dogs are part of the disease's life cycle and they may also serve as sentinel animals in epidemiological studies. The first human BSF case in the State of Paraná was reported in 2005. The present study was conducted in the municipality of Almirante Tamandaré, where no previous case of BSF was reported. Serum samples were collected from 71 horses and 20 dogs from nine properties in the area. Ticks were also collected from these animals. All farmers completed a questionnaire about their knowledge of BSF and animal health management. Serum samples were analyzed by indirect immunofluorescent-antibody assay (IFA using R. rickettsii and R. parkeri as antigens. Ticks were analyzed by PCR for Rickettsia sp., and all of them were PCR-negative. Six horses (8.45% and 4 dogs (20% were identified as seropositive. Farmers were not aware of the correlation between the presence of ticks and risk of BSF. Although a non-endemic area, Almirante Tamandaré is a vulnerable environment for BSF and effective tick control measures are required.A Febre Maculosa Brasileira (FMB é uma riquetsiose letal para humanos, causada pela bactéria Rickettsia rickettsii, e é endêmica em algumas regiões brasileiras. Equinos e cães podem participar do ciclo da doença e podem também servir como sentinelas em estudos epidemiológicos. O primeiro caso humano relatado no Estado do Paraná ocorreu em 2005. O presente estudo foi realizado no município de Almirante Tamandaré, região onde não há relatos de casos de FMB. Foram coletadas amostras de sangue de 71 cavalos e 20 cães em nove propriedades rurais na região. Carrapatos também foram colhidos dos animais. Todos os proprietários responderam a um questionário sobre o manejo sanitário dos animais e o conhecimento a respeito da FMB. As amostras de soro foram processadas pela técnica de Reação de Imunofluorescência Indireta (RIFI, utilizando-se os antígenos de R. rickettsii e R. parkeri. Os carrapatos foram analisados por PCR para Rickettsia sp. e todos foram negativos. Seis cavalos (8,45% e 4 cães (20% foram identificados como soropositivos. Todos os proprietários desconheciam a relação de carrapatos com a FMB. Embora considerada uma área não endêmica, Almirante Tamandaré é um ambiente vulnerável à FMB e um controle eficiente de carrapatos deve ser implementado.

Fernanda Gonçalves Batista

2010-12-01

166

Evidencia serológica de la presencia de Rickettsias del grupo de la fiebre manchada en la Amazonía del Perú / Serological evidence of spotted fever group-related Rickettsia transmission in the Peruvian Amazon jungle  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Con el objetivo de demostrar la existencia de transmisión de Rickettsias del grupo de la fiebre manchada en la Amazonía peruana, se tomaron muestras de sangre a pacientes febriles agudos en establecimientos de salud de la ciudad de Iquitos, la ciudad más poblada de la Amazonía del Perú. Las muestras [...] fueron procesadas mediante inmunofluorescencia indirecta para medir anticuerpos totales e IgG específica para el grupo de fiebre de las manchadas. Entre enero y julio de 2006, se obtuvieron muestras de 250 pacientes. El 37% de las muestras tuvieron títulos positivos de IgG, demostrando así haber tenido contacto con el agente, de ellas, nueve fueron clasificadas como casos agudos, en los que se descartó otras infecciones endémicas como dengue, malaria y leptospirosis. Los casos presentaron una enfermedad febril acompañada de síntomas como tos, sarpullido y hemoptisis. Cuatro casos fueron hospitalizados, dos fueron graves y uno de ellos falleció. En conclusión, existe evidencia serológica de la circulación de Rickettsias del grupo de las fiebre manchada en la Amazonía peruana, por su frecuencia y potencial gravedad debería ser tomada en cuenta como diagnóstico diferencial del síndrome febril agudo en esta región. Abstract in english Blood samples were taken from acutely ill febrile patients in health centers in Iquitos, the most densely populated city in Peruvian Amazon jungle, in order to prove the transmission of spotted fever group-related Rickettsia transmission. Samples were processed using indirect immunofluorescence for [...] measuring total antibodies and specific IgG for the spotted fever group. Between January and July 2006, 250 patients had samples taken. 37% had positive IgG titers showing that these patients had contact with the agent; and out of them, nine were adjudicated as acute cases, where other endemic conditions were ruled out, such as dengue fever, malaria, and leptospirosis. Cases identified had a febrile illness with the following concomitant symptoms and signs: cough, skin rash, and hemoptysis. Four patients were hospitalized, two had severe illness, and one of them ultimately died. We conclude that there is evidence of spotted fever group-related Rickettsia circulation in the Peruvian Amazon jungle, so this condition should be taken into account as a differential diagnosis for acute febrile syndrome in this region.

Sihuincha M, Moisés; Anaya F, Elizabeth; Carranza V, Victoria; Durand V, Salomón.

167

Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil / Identificação sorológica de Rickettsia sp. em equinos e cães de área não endêmica no Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese A Febre Maculosa Brasileira (FMB) é uma riquetsiose letal para humanos, causada pela bactéria Rickettsia rickettsii, e é endêmica em algumas regiões brasileiras. Equinos e cães podem participar do ciclo da doença e podem também servir como sentinelas em estudos epidemiológicos. O primeiro caso human [...] o relatado no Estado do Paraná ocorreu em 2005. O presente estudo foi realizado no município de Almirante Tamandaré, região onde não há relatos de casos de FMB. Foram coletadas amostras de sangue de 71 cavalos e 20 cães em nove propriedades rurais na região. Carrapatos também foram colhidos dos animais. Todos os proprietários responderam a um questionário sobre o manejo sanitário dos animais e o conhecimento a respeito da FMB. As amostras de soro foram processadas pela técnica de Reação de Imunofluorescência Indireta (RIFI), utilizando-se os antígenos de R. rickettsii e R. parkeri. Os carrapatos foram analisados por PCR para Rickettsia sp. e todos foram negativos. Seis cavalos (8,45%) e 4 cães (20%) foram identificados como soropositivos. Todos os proprietários desconheciam a relação de carrapatos com a FMB. Embora considerada uma área não endêmica, Almirante Tamandaré é um ambiente vulnerável à FMB e um controle eficiente de carrapatos deve ser implementado. Abstract in english Brazilian Spotted Fever (BSF) is a lethal rickettsiosis in humans caused by the bacteria Rickettsia rickettsii, and is endemic in some areas of Brazil. Horses and dogs are part of the disease's life cycle and they may also serve as sentinel animals in epidemiological studies. The first human BSF cas [...] e in the State of Paraná was reported in 2005. The present study was conducted in the municipality of Almirante Tamandaré, where no previous case of BSF was reported. Serum samples were collected from 71 horses and 20 dogs from nine properties in the area. Ticks were also collected from these animals. All farmers completed a questionnaire about their knowledge of BSF and animal health management. Serum samples were analyzed by indirect immunofluorescent-antibody assay (IFA) using R. rickettsii and R. parkeri as antigens. Ticks were analyzed by PCR for Rickettsia sp., and all of them were PCR-negative. Six horses (8.45%) and 4 dogs (20%) were identified as seropositive. Farmers were not aware of the correlation between the presence of ticks and risk of BSF. Although a non-endemic area, Almirante Tamandaré is a vulnerable environment for BSF and effective tick control measures are required.

Batista, Fernanda Gonçalves; Silva, Daniella Matos da; Green, Kerriel Thandile; Tezza, Louise Boulsfield de Lorenzi; Vasconcelos, Sâmara Pereira de; Carvalho, Suelen Graziele Soares de; Silveira, Iara; Moraes-Filho, Jonas; Labruna, Marcelo Bahia; Fortes, Fernanda Silva; Molento, Marcelo Beltrão.

168

Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil Characterization of Rickettsia spp. circulating in a silent peri-urban focus for Brazilian spotted fever in Caratinga, Minas Gerais, Brazil  

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Full Text Available O objetivo deste trabalho foi caracterizar Rickettsia spp. circulante em artrópodes vetores no Município de Caratinga, Minas Gerais, Brasil, por meio da PCR, e investigar a presença de anticorpos para riquétsias do grupo da febre maculosa em cães e eqüinos. 2.610 ectoparasitos foram coletados e identificados taxonomicamente. Amostras de DNA obtidas desses vetores foram submetidas à PCR e seqüenciamento. Em pulgas do gênero Ctenocephalides e em carrapatos Amblyomma cajennense foram identificadas seqüências com 100% de homologia com R. felis. Em carrapatos Rhipicephalus sanguineus uma seqüência apresentou 99% de homologia com R. felis e uma seqüência obtida de A. cajennense apresentou 97% de homologia com R. honei e R. rickettsii. Soros de cães (73 e de eqüinos (18 foram submetidos à imunofluorescência indireta (RIFI usando-se antígeno de R. rickettsii. Apenas três dos soros de eqüinos (17% mostraram-se positivos. A detecção molecular de riquetsias potencialmente patogênicas ao homem em vetores e a presença de sororeatividade para riquetsias do grupo da febre maculosa em eqüinos, demonstram o risco de transmissão de riquetsioses nessa área e a necessidade de se manter um sistema contínuo de vigilância epidemiológica.The present study was intended to characterize Rickettsia spp. circulating in arthropod vectors in Caratinga, Minas Gerais, Brazil, by PCR and to investigate the presence of antibodies against the spotted fever Rickettsiae group (SFRG in dogs and horses. 2,610 arthropods were collected and taxonomically identified. DNA samples obtained from these vectors were submitted to PCR and cycle-sequenced. Ctenocephalides and Amblyomma cajennense showed sequences presenting 100.0% homology with R. felis. A sequence obtained from Rhipicephalus sanguineus showed 99.0% homology with R. felis, and a sequence from A. cajennense showed 97.0% homology with R. honei and R. rickettsii. Canine (73 and equine (18 serum samples were tested by indirect fluorescent antibody (IFA using R. rickettsii antigen. Only three of the equine sera tested (17.0% had positive antibody titers. Molecular detection of rickettsiae species potentially pathogenic to humans in arthropod vectors and the presence of seroreactivity to SFRG in horses show the risk of transmission of rickettsiosis in this area and the need to maintain continuous epidemiological surveillance for rickettsial diseases.

Luciane Daniele Cardoso

2006-03-01

169

Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil / Characterization of Rickettsia spp. circulating in a silent peri-urban focus for Brazilian spotted fever in Caratinga, Minas Gerais, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Public Health | Language: Portuguese Abstract in portuguese O objetivo deste trabalho foi caracterizar Rickettsia spp. circulante em artrópodes vetores no Município de Caratinga, Minas Gerais, Brasil, por meio da PCR, e investigar a presença de anticorpos para riquétsias do grupo da febre maculosa em cães e eqüinos. 2.610 ectoparasitos foram coletados e iden [...] tificados taxonomicamente. Amostras de DNA obtidas desses vetores foram submetidas à PCR e seqüenciamento. Em pulgas do gênero Ctenocephalides e em carrapatos Amblyomma cajennense foram identificadas seqüências com 100% de homologia com R. felis. Em carrapatos Rhipicephalus sanguineus uma seqüência apresentou 99% de homologia com R. felis e uma seqüência obtida de A. cajennense apresentou 97% de homologia com R. honei e R. rickettsii. Soros de cães (73) e de eqüinos (18) foram submetidos à imunofluorescência indireta (RIFI) usando-se antígeno de R. rickettsii. Apenas três dos soros de eqüinos (17%) mostraram-se positivos. A detecção molecular de riquetsias potencialmente patogênicas ao homem em vetores e a presença de sororeatividade para riquetsias do grupo da febre maculosa em eqüinos, demonstram o risco de transmissão de riquetsioses nessa área e a necessidade de se manter um sistema contínuo de vigilância epidemiológica. Abstract in english The present study was intended to characterize Rickettsia spp. circulating in arthropod vectors in Caratinga, Minas Gerais, Brazil, by PCR and to investigate the presence of antibodies against the spotted fever Rickettsiae group (SFRG) in dogs and horses. 2,610 arthropods were collected and taxonomi [...] cally identified. DNA samples obtained from these vectors were submitted to PCR and cycle-sequenced. Ctenocephalides and Amblyomma cajennense showed sequences presenting 100.0% homology with R. felis. A sequence obtained from Rhipicephalus sanguineus showed 99.0% homology with R. felis, and a sequence from A. cajennense showed 97.0% homology with R. honei and R. rickettsii. Canine (73) and equine (18) serum samples were tested by indirect fluorescent antibody (IFA) using R. rickettsii antigen. Only three of the equine sera tested (17.0%) had positive antibody titers. Molecular detection of rickettsiae species potentially pathogenic to humans in arthropod vectors and the presence of seroreactivity to SFRG in horses show the risk of transmission of rickettsiosis in this area and the need to maintain continuous epidemiological surveillance for rickettsial diseases.

Cardoso, Luciane Daniele; Freitas, Renata Nascimento; Mafra, Cláudio Lísias; Neves, Cristiane Vilas Boas; Figueira, Fátima Cristina Bacellar; Labruna, Marcelo Bahia; Gennari, Solange M.; Walker, David Hughes; Galvão, Márcio Antônio Moreira.

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Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil / Characterization of Rickettsia spp. circulating in a silent peri-urban focus for Brazilian spotted fever in Caratinga, Minas Gerais, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O objetivo deste trabalho foi caracterizar Rickettsia spp. circulante em artrópodes vetores no Município de Caratinga, Minas Gerais, Brasil, por meio da PCR, e investigar a presença de anticorpos para riquétsias do grupo da febre maculosa em cães e eqüinos. 2.610 ectoparasitos foram coletados e iden [...] tificados taxonomicamente. Amostras de DNA obtidas desses vetores foram submetidas à PCR e seqüenciamento. Em pulgas do gênero Ctenocephalides e em carrapatos Amblyomma cajennense foram identificadas seqüências com 100% de homologia com R. felis. Em carrapatos Rhipicephalus sanguineus uma seqüência apresentou 99% de homologia com R. felis e uma seqüência obtida de A. cajennense apresentou 97% de homologia com R. honei e R. rickettsii. Soros de cães (73) e de eqüinos (18) foram submetidos à imunofluorescência indireta (RIFI) usando-se antígeno de R. rickettsii. Apenas três dos soros de eqüinos (17%) mostraram-se positivos. A detecção molecular de riquetsias potencialmente patogênicas ao homem em vetores e a presença de sororeatividade para riquetsias do grupo da febre maculosa em eqüinos, demonstram o risco de transmissão de riquetsioses nessa área e a necessidade de se manter um sistema contínuo de vigilância epidemiológica. Abstract in english The present study was intended to characterize Rickettsia spp. circulating in arthropod vectors in Caratinga, Minas Gerais, Brazil, by PCR and to investigate the presence of antibodies against the spotted fever Rickettsiae group (SFRG) in dogs and horses. 2,610 arthropods were collected and taxonomi [...] cally identified. DNA samples obtained from these vectors were submitted to PCR and cycle-sequenced. Ctenocephalides and Amblyomma cajennense showed sequences presenting 100.0% homology with R. felis. A sequence obtained from Rhipicephalus sanguineus showed 99.0% homology with R. felis, and a sequence from A. cajennense showed 97.0% homology with R. honei and R. rickettsii. Canine (73) and equine (18) serum samples were tested by indirect fluorescent antibody (IFA) using R. rickettsii antigen. Only three of the equine sera tested (17.0%) had positive antibody titers. Molecular detection of rickettsiae species potentially pathogenic to humans in arthropod vectors and the presence of seroreactivity to SFRG in horses show the risk of transmission of rickettsiosis in this area and the need to maintain continuous epidemiological surveillance for rickettsial diseases.

Cardoso, Luciane Daniele; Freitas, Renata Nascimento; Mafra, Cláudio Lísias; Neves, Cristiane Vilas Boas; Figueira, Fátima Cristina Bacellar; Labruna, Marcelo Bahia; Gennari, Solange M.; Walker, David Hughes; Galvão, Márcio Antônio Moreira.

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Primary isolation of spotted fever group rickettsiae from Amblyomma cooperi collected from Hydrochaeris hydrochaeris in Brazil  

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Full Text Available This paper reports the first isolation of a spotted fever group rickettsia from an Amblyomma cooperi ixodid collected from a capybara (Hydrochaeris hydrochaeris in an endemic area of spotted fever in the County of Pedreira, State of São Paulo, Brazil. Isolation was performed in Vero cell culture and submitted to immunofluorescence, using antibody from Rickettsia rickettsii-positive human serum.

Elba Regina Sampaio de Lemos

1996-06-01

172

Comparison of different media for preservation and transport of viable rickettsiae.  

Science.gov (United States)

Rickettsiae tend to have a rapid decrease of viability outside living cells. Therefore, the transport of samples containing viable rickettsiae for culturing in cell culture for diagnostic purposes is challenging. The viability of rickettsiae in different transport media (commercially available transport medium COPAN "UTM-RT transport medium for viruses, chlamydia, mycoplasma, and ureaplasma," minimal essential medium (MEM) with and without 10% foetal calf serum) at various time points at 4 °C and at ambient temperature (22 °C) was compared. Rickettsia honei was used as model organism. After 2 weeks of storage at room temperature, no viable rickettsiae were detectable any more while storage at 4 °C kept rickettsiae viable for up to 4 weeks. The commercially available COPAN medium showed similarly good or slightly better stabilizing effects on rickettsiae compared with MEM + 10% foetal calf serum, pure MEM demonstrated the poorest results. It is important to transport and store media with potentially rickettsiae-containing samples at 4 °C to prevent inactivation. MEM + 10% foetal calf serum can be used if no commercial medium is available with similarly good results. PMID:24265938

Frickmann, Hagen; Dobler, Gerhard

2013-09-01

173

Genome Sequence of Rickettsia australis, the Agent of Queensland Tick Typhus  

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Rickettsia australis strain PhillipsT was isolated in Queensland, Australia, in 1950. It is the tick-borne agent of Queensland tick typhus, a disease endemic in Australia. The 1.29-Mb genome sequence of this bacterium is highly similar to that of Rickettsia akari but contains two plasmids.

Dong, Xin; El Karkouri, Khalid; Robert, Catherine; Raoult, Didier; Fournier, Pierre-edouard

2012-01-01

174

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples  

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A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Rickettsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

Svendsen, Claus Bo; Boye, Mette; Struve, Carsten; Krogfelt, Karen A.

2009-01-01

175

Rickettsia typhi Infection with Interstitial Pneumonia in a Traveler Treated with Moxifloxacin?  

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Rickettsial diseases may play an important part in the differential diagnosis of fever in returned travelers. The initial empirical treatment needs to take Rickettsia species into consideration to avoid the development of life-threatening courses. Here, we present a case of interstitial pneumonia associated with Rickettsia typhi infection treated with moxifloxacin.

Schulze, Marco H.; Keller, Christian; Mu?ller, Andreas; Ziegler, Uwe; Langen, Heinz-jakob; Hegasy, Guido; Stich, August

2011-01-01

176

Complete Genome Sequence of Rickettsia slovaca, the Agent of Tick-Borne Lymphadenitis  

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The present study reports the complete and annotated genome sequence of the human pathogen Rickettsia slovaca strain 13-B, which was isolated from a Dermacentor tick in Slovakia in 1968. The 1.27-Mb genome provides further insights into the acquisition of virulence related to genome reduction in Rickettsia species.

Fournier, Pierre-edouard; El Karkouri, Khalid; Robert, Catherine; Me?digue, Claudine; Raoult, Didier

2012-01-01

177

Rickettsia spp. in Seabird Ticks from Western Indian Ocean Islands, 2011-2012.  

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We found a diversity of Rickettsia spp. in seabird ticks from 6 tropical islands. The bacteria showed strong host specificity and sequence similarity with strains in other regions. Seabird ticks may be key reservoirs for pathogenic Rickettsia spp., and bird hosts may have a role in dispersing ticks and tick-associated infectious agents over large distances. PMID:24751287

Dietrich, Muriel; Lebarbenchon, Camille; Jaeger, Audrey; Le Rouzic, Céline; Bastien, Matthieu; Lagadec, Erwan; McCoy, Karen D; Pascalis, Hervé; Le Corre, Matthieu; Dellagi, Koussay; Tortosa, Pablo

2014-05-01

178

Use of immunoradiometric analysis to determine Rickettsia antigens in cell cultures and chick embryos  

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A modification of an immunoradiometric analysis to determine Rickettsia antigens in various biological substrates was studied, using rickettsious diagnostricums, egg and cell cultures of Rickettsia. The method was highly sensitive for the determination of minimal quantities of antigens in these substrates. The method appears to be promising for studies related to the detection of microorganisms and their antigens. 5 references.

Prozorovskiy, S.V.; Alekseeva, N.V.; Knyazeva, E.N.; Ignatovich, V.F.; Barkhatova, O.T.

1984-02-01

179

Serological Survey of Rickettsia japonica Infection in Dogs and Cats in Japan?  

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Antibodies against Rickettsia japonica in 20 of 1,207 dogs and 5 of 584 cats in Japan were detected using immunofluorescence. Some antibody-positive animals were detected in Niigata and Kagawa Prefectures, areas in which Japanese spotted fever in human patients has never been identified. Some animals were positive for antibodies against other new Rickettsia species.

2007-01-01

180

Primary isolation of spotted fever group rickettsiae from Amblyomma cooperi collected from Hydrochaeris hydrochaeris in Brazil  

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This paper reports the first isolation of a spotted fever group rickettsia from an Amblyomma cooperi ixodid collected from a capybara (Hydrochaeris hydrochaeris) in an endemic area of spotted fever in the County of Pedreira, State of São Paulo, Brazil. Isolation was performed in Vero cell culture and submitted to immunofluorescence, using antibody from Rickettsia rickettsii-positive human serum.

1996-01-01

 
 
 
 
181

Molecular characterization of a novel Rickettsia species from Ixodes scapularis in Texas.  

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A novel Rickettsia species of undetermined pathogenicity was detected in Ixodes scapularis. DNA sequencing showed the highest nucleotide sequence similarities with R. australis for the 17 kDa gene, R. helvetica for gltA, and R. montana for rompA. The new organism, provisionally designated as genotype Cooleyi, is highly divergent in three conserved genes from recognized Rickettsia species.

Billings, A. N.; Teltow, G. J.; Weaver, S. C.; Walker, D. H.

1998-01-01

182

Factores morfológicos asociados al pronóstico de pacientes operados por cáncer gástrico incipiente Pathological features associated with survival in early gastric cancer  

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Introducción: El cáncer gástrico incipiente (CGI) es aquel que compromete la mucosa o submucosa gástrica independientemente del compromiso ganglionar linfático, estimándose su prevalencia en Chile inferior al 20%o. El objetivo de este estudio es determinar prevalencia de CGI y asociación de variables biode-mográficas y morfológicas con la supervivencia (SV) de pacientes resecados por CGI. Material y Método: Estudio de cohorte retrospectiva. Se estudiaron variables biodemográficas y...

2011-01-01

183

Serological evidence of Rickettsia parkeri as the etiological agent of rickettsiosis in Uruguay Evidência sorológica de Rickettsia parkeri como agente etiológico de rickettsiose no Uruguai  

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We report three new rickettsiosis human cases in Uruguay. The three clinical cases presented clinical manifestations similar to previous reported cases of Rickettsia parkeri in the United States; that is mild fever (< 40 ºC), malaise, headache, rash, inoculation eschar at the tick bite site, regional lymphadenopathy, and no lethality. Serological antibody-absorption tests with purified antigens of R. parkeri and Rickettsia rickettsii, associated with immunofluorescence assay indicated that t...

Conti-di?az, Ismael A.; Jonas Moraes-Filho; Pacheco, Richard C.; Labruna, Marcelo B.

2009-01-01

184

Coexistência de linfadenite axilar tuberculosa e metástase ganglionar de carcinoma lobular de mama: relato de um caso / Coexistence of axillary tuberculous lymphadenitis and ganglionic metastasis in mammary lobular carcinoma: a case report  

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Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Relato do caso de uma mulher com 83 anos apresentando nódulo e retração de pele na mama direita com oito meses de evolução. Ao exame físico verificou-se nódulo sólido de 5 cm, localizado no quadrante súpero-lateral de mama direita, associado a presença de retração de pele correspondente e linfonodos [...] axilares não coalescentes ipsilaterais. O resultado da mamografia evidenciou nódulo de 4 cm de diâmetro irregular no quadrante súpero-lateral da mama direita (bi-rads V). Estádio clínico: T2N1M0 (IIB). O tratamento cirúrgico incluiu mastectomia radical modificada (à Maden) com dissecção axilar níveis I, II e III. Avaliação histopatológica demonstrou a presença de carcinoma lobular infiltrativo que mediu 2,5 cm (T2), presença de linfadenite granulomatosa causada por tuberculose em linfonodos dos níveis I, II e III, associados a metástase de carcinoma lobular em um único nível linfático, nível I. Estádio patológico: pT2pN1aM0. A paciente recebeu tratamento para tuberculose ganglionar com rifampicina, isoniazida e pirazinamida por um ano. Foram solicitados receptores hormonais, os quais mostraram-se positivos, sendo feito terapia adjuvante com tamoxifeno. Durante o primeiro ano de seguimento a paciente evoluiu bem, sem sinais de recidiva local ou metástases a distância. Abstract in english Report of a case of an 83-year-old woman presenting a nodule and skin retraction in the right breast for eight months. On physical examination, a solid nodule of 5 cm was observed, located in the upper-lateral quadrant of the right breast, associated with skin retraction and ipsilateral lymph nodes. [...] Mammographic findings showed irregularly limited nodules of 4 cm in the upper-lateral quadrant of the right breast (bi-rads V). Clinical staging: T2N1M0 (IIB). Surgical treatment included a modified radical mastectomy with axillary dissection levels I, II, and III. Histopathologic evaluation demonstrated the presence of an infiltrating lobular carcinoma measuring 2.5 cm (T2), presence of granulomatous lymphadenitis caused by tuberculosis in level I, II, and III lymph nodes, associated with lobular carcinoma metastasis in a single level I lymph node. Pathologic staging: pT2pN1aM0. The treatment for the axillary tuberculous lymphadenitis was done with rifampin, isoniazid and pyrazinamide for one year. Hormone receptors were positive, and adjuvant therapy was initiated with tamoxifen. During the first year of follow-up the patient had no signal of local recurrence or distant metastases.

José Juvenal, Linhares; Eduardo Camargo, Millen; Marcelo, Antonini; Pedro César, Fagundes; Pedro Gustavo, Falcão; Joaquim Teodoro de, Araújo Neto.

185

Sca1, a previously undescribed paralog from autotransporter protein-encoding genes in Rickettsia species  

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Full Text Available Abstract Background Among the 17 genes encoding autotransporter proteins of the "surface cell antigen" (sca family in the currently sequenced Rickettsia genomes, ompA, sca5 (ompB and sca4 (gene D, have been extensively used for identification and phylogenetic purposes for Rickettsia species. However, none of these genes is present in all 20 currently validated Rickettsia species. Of the remaining 14 sca genes, sca1 is the only gene to be present in all nine sequenced Rickettsia genomes. To estimate whether the sca1 gene is present in all Rickettsia species and its usefulness as an identification and phylogenetic tool, we searched for sca1genes in the four published Rickettsia genomes and amplified and sequenced this gene in the remaining 16 validated Rickettsia species. Results Sca1 is the only one of the 17 rickettsial sca genes present in all 20 Rickettsia species. R. prowazekii and R. canadensis exhibit a split sca1 gene whereas the remaining species have a complete gene. Within the sca1 gene, we identified a 488-bp variable sequence fragment that can be amplified using a pair of conserved primers. Sequences of this fragment are specific for each Rickettsia species. The phylogenetic organization of Rickettsia species inferred from the comparison of sca1 sequences strengthens the classification based on the housekeeping gene gltA and is similar to those obtained from the analyses of ompA, sca5 and sca4, thus suggesting similar evolutionary constraints. We also observed that Sca1 protein sequences have evolved under a dual selection pressure: with the exception of typhus group rickettsiae, the amino-terminal part of the protein that encompasses the predicted passenger domain, has evolved under positive selection in rickettsiae. This suggests that the Sca1 protein interacts with the host. In contrast, the C-terminal portion containing the autotransporter domain has evolved under purifying selection. In addition, sca1 is transcribed in R. conorii, and might therefore be functional in this species. Conclusion The sca1 gene, encoding an autotransporter protein that evolves under dual evolution pressure, is the only sca-family gene to be conserved by all Rickettsia species. As such, it is a valuable identification target for these bacteria, especially because rickettsial isolates can be identified by amplification and sequencing of a discriminatory gene fragment using a single primer pair. It may also be used as a phylogenetic tool. However, its current functional status remains to be determined although it was found expressed in R. conorii.

Raoult Didier

2006-02-01

186

Gene gain and loss events in Rickettsia and Orientia species  

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Full Text Available Abstract Background Genome degradation is an ongoing process in all members of the Rickettsiales order, which makes these bacterial species an excellent model for studying reductive evolution through interspecies variation in genome size and gene content. In this study, we evaluated the degree to which gene loss shaped the content of some Rickettsiales genomes. We shed light on the role played by horizontal gene transfers in the genome evolution of Rickettsiales. Results Our phylogenomic tree, based on whole-genome content, presented a topology distinct from that of the whole core gene concatenated phylogenetic tree, suggesting that the gene repertoires involved have different evolutionary histories. Indeed, we present evidence for 3 possible horizontal gene transfer events from various organisms to Orientia and 6 to Rickettsia spp., while we also identified 3 possible horizontal gene transfer events from Rickettsia and Orientia to other bacteria. We found 17 putative genes in Rickettsia spp. that are probably the result of de novo gene creation; 2 of these genes appear to be functional. On the basis of these results, we were able to reconstruct the gene repertoires of "proto-Rickettsiales" and "proto-Rickettsiaceae", which correspond to the ancestors of Rickettsiales and Rickettsiaceae, respectively. Finally, we found that 2,135 genes were lost during the evolution of the Rickettsiaceae to an intracellular lifestyle. Conclusions Our phylogenetic analysis allowed us to track the gene gain and loss events occurring in bacterial genomes during their evolution from a free-living to an intracellular lifestyle. We have shown that the primary mechanism of evolution and specialization in strictly intracellular bacteria is gene loss. Despite the intracellular habitat, we found several horizontal gene transfers between Rickettsiales species and various prokaryotic, viral and eukaryotic species. Open peer review Reviewed by Arcady Mushegian, Eugene V. Koonin and Patrick Forterre. For the full reviews please go to the Reviewers' comments section.

Raoult Didier

2011-02-01

187

Radioiodination of an outer membrane protein in intact Rickettsia prowazekii  

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Intact Rickettsia prowazekii was radiolabeled with the glucose oxidase-lactoperoxidase method of iodination. Separation of the rickettsial extract into cytoplasmic, outer and inner membrane fractions demonstrated that the outer membrane was preferentially labeled. Analysis of the polypeptides of these fractions on high-resolution slab polyacrylamide gels showed that most of the "1"2"5I was in polypeptide T49, an outer membrane constituent. Additional outer membrane polypeptides were iodinated in broken envelope preparations, demonstrating that T49 is uniquely accessible to the external environment and the asymmetric polypeptide organization of the outer membrane

1980-01-01

188

Radioiodination of an outer membrane protein in intact Rickettsia prowazekii  

Energy Technology Data Exchange (ETDEWEB)

Intact Rickettsia prowazekii was radiolabeled with the glucose oxidase-lactoperoxidase method of iodination. Separation of the rickettsial extract into cytoplasmic, outer and inner membrane fractions demonstrated that the outer membrane was preferentially labeled. Analysis of the polypeptides of these fractions on high-resolution slab polyacrylamide gels showed that most of the /sup 125/I was in polypeptide T49, an outer membrane constituent. Additional outer membrane polypeptides were iodinated in broken envelope preparations, demonstrating that T49 is uniquely accessible to the external environment and the asymmetric polypeptide organization of the outer membrane.

Smith, D.K.; Winkler, H.H.

1980-08-01

189

Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas.  

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The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both ...

Azad, A. F.; Sacci, J. B.; Nelson, W. M.; Dasch, G. A.; Schmidtmann, E. T.; Carl, M.

1992-01-01

190

The first finding of a Rickettsia bacterium associated with parthenogenesis induction among insects.  

Science.gov (United States)

The larval endoparasitoid, Neochrysocharis formosa (Westwood), is an important natural enemy of the leafminer Liriomyza trifolii (Burgess) in Japan. The thelytokous strain of N. formosa mostly produces female progeny. Male progeny were produced by females treated with tetracycline, suggesting that microorganisms induce thelytoky in N. formosa. The result of nucleotide sequencing of the 16S rRNA gene indicated that the parasitoid is infected with a Rickettsia bacterium, which appears to be causative of the thelytoky. Although Rickettsia-bellii-like bacteria have been found to be associated with various reproductive disorders, this is the first finding of a parthenogenesis-inducing Rickettsia among insects. PMID:16450063

Hagimori, Tetsuya; Abe, Yoshihisa; Date, Shuichi; Miura, Kazuki

2006-02-01

191

Morphometric and quantitative characterization of atrial ganglion neurons from the intercaval region in dogs with dilated cardiomyopathy Caracterização morfométrica e quantitativa dos neurônios ganglionares atriais da faixa intercaval de cães com cardiomiopatia dilatada  

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Full Text Available The quantity, morphometry, and quality of atrial neurons from the intercaval region in dogs with dilated cardiomyopathy (DCM were evaluated. Dogs with DCM had greater ganglion neurons than control dogs. The histologic evaluation of the ventricular myocardium and ganglion neurons confirmed DCM and showed the degeneration of ganglion neurons. Dogs with chronic DCM had a secondary cardioneuropathy owing to impaired parasympathetic neural control.Avaliaram-se quantitativa, morfométrica e qualitativamente os neurônios atriais da faixa intercaval de cães com cardiomiopatia dilatada (CMD. Os neurônios dos gânglios nervosos de cães com CMD eram maiores que os dos cães controle. A histopatologia do miocárdio ventricular e dos neurônios ganglionares confirmou a CMD e demonstrou evidente processo degenerativo neuronal ganglionar. Cães com CMD em fase crônica apresentavam cardioneuropatia secundária, provavelmente pela privação da inervação parassimpática cardíaca.

A.A. Camacho

2007-12-01

192

An update on the detection and treatment of Rickettsia felis  

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Full Text Available Laya Hun, Adriana TroyoCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa RicaAbstract: Rickettsia felis was described as a human pathogen almost two decades ago, and human infection is currently reported in 18 countries in all continents. The distribution of this species is worldwide, determined by the presence of the main arthropod vector, Ctenocephalides felis (Bouché. The list of symptoms, which includes fever, headache, myalgia, and rash, keeps increasing as new cases with unexpected symptoms are described. Moreover, the clinical presentation of R. felis infection can be easily confused with many tropical and nontropical diseases, as well as other rickettsial infections. Although specific laboratory diagnosis and treatment for this flea-borne rickettsiosis are detailed in the scientific literature, it is possible that most human cases are not being diagnosed properly. Furthermore, since the cat flea infests different common domestic animals, contact with humans may be more frequent than reported. In this review, we provide an update on methods for specific detection of human infection by R. felis described in the literature, as well as the treatment prescribed to the patients. Considering advances in molecular detection tools, as well as options for as-yet-unreported isolation of R. felis from patients in cell culture, increased diagnosis and characterization of this emerging pathogen is warranted.Keywords: Rickettsia felis, human cases, laboratory diagnosis, treatment

Hun L

2012-06-01

193

Experimental infection of the opossum Didelphis aurita by Rickettsia felis, Rickettsia bellii, and Rickettsia parkeri and evaluation of the transmission of the infection to ticks Amblyomma cajennense and Amblyomma dubitatum.  

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This work evaluated the infection of opossums (Didelphis aurita) by Rickettsia felis, Rickettsia bellii, and Rickettsia parkeri and their role as amplifier hosts for horizontal transmission to Amblyomma cajennense and/or Amblyomma dubitatum ticks. Infection in D. aurita was induced by intraperitoneal inoculation with R. felis (n?=?4 opossums), R. bellii (n?=?4), and R. parkeri (n?=?2). Another group of six opossums were inoculated intraperitoneally with Leibovitz-15 sterile culture medium, representing the uninfected groups (n?=?2 opossums simultaneously to each infected group). Opossum blood samples collected during the study were used for DNA extraction, followed by real-time polymerase chain reaction targeting the rickettsial gene gltA, hematology, and detection of Rickettsia spp.-reactive antibodies by indirect immunofluorescence assay. Opossums were infested with uninfected A. cajennense and/or A. dubitatum for 30 days postinoculation (DPI). Flat ticks molted from ticks fed on opossums were allowed to feed on uninfected rabbits, which were tested for seroconversion by immunofluorescence assay. Samples of flat ticks were also tested by real-time polymerase chain reaction. Inoculated opossums showed no clinical abnormalities. Antibodies to Rickettsia spp. were first detected at the second to fourth DPI, with detectable titers until the 150th DPI. Rickettsemia was detected only in one opossum inoculated with R. parkeri, at the eighth DPI. Only one A. cajennense tick (2.0%) previously fed on a R. parkeri-inoculated opossum became infected. None of the rabbits infested with opossum-derived ticks seroconverted. The study demonstrated that R. felis, R. bellii, and R. parkeri were capable to produce antibody response in opossums, however, with undetectable rickettsemia for R. felis and R. bellii, and very low rickettsemia for R. parkeri. Further studies must be done with different strains of these rickettsiae, most importantly the strains that have never gone through in vitro passages. PMID:20455783

Horta, Maurício C; Sabatini, Guilherme S; Moraes-Filho, Jonas; Ogrzewalska, Maria; Canal, Raoní B; Pacheco, Richard C; Martins, Thiago F; Matushima, Eliana R; Labruna, Marcelo B

2010-12-01

194

Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reduction  

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Full Text Available Abstract Background The Rickettsia genus includes 25 validated species, 17 of which are proven human pathogens. Among these, the pathogenicity varies greatly, from the highly virulent R. prowazekii, which causes epidemic typhus and kills its arthropod host, to the mild pathogen R. africae, the agent of African tick-bite fever, which does not affect the fitness of its tick vector. Results We evaluated the clonality of R. africae in 70 patients and 155 ticks, and determined its genome sequence, which comprises a circular chromosome of 1,278,540 bp including a tra operon and an unstable 12,377-bp plasmid. To study the genetic characteristics associated with virulence, we compared this species to R. prowazekii, R. rickettsii and R. conorii. R. africae and R. prowazekii have, respectively, the less and most decayed genomes. Eighteen genes are present only in R. africae including one with a putative protease domain upregulated at 37°C. Conclusion Based on these data, we speculate that a loss of regulatory genes causes an increase of virulence of rickettsial species in ticks and mammals. We also speculate that in Rickettsia species virulence is mostly associated with gene loss. The genome sequence was deposited in GenBank under accession number [GenBank: NZ_AAUY01000001].

Audic Stéphane

2009-04-01

195

Clinical and laboratorial evidence of Rickettsia felis infections in Latin America  

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Full Text Available After the discovery and initial characterization of Rickettsia felis in 1992 by Azad and cols, and the subsequent first description of a human case of infection in 1994, there have been two communications of human rickettsiosis cases caused by Rickettsia felis in Latin America. The first one was published in 2000 by Zavala-Velazquez and cols in Mexico. In 2001 Raoult and cols described the occurrence of two human cases of Rickettsia felis rickettsiosis in Brazil. In the present discussion these two articles were compared and after the description of the principal signs and symptoms, it was concluded that more studies are needed with descriptions of a greater number of patients to establish the true frequency of the clinical signs and symptoms present in Rickettsia felis rickettsiosis.

Galvão Márcio Antônio Moreira

2004-01-01

196

[Isolation of a novel rickettsia from the tick Dermacentor silvarum in Baikal lake region].  

Science.gov (United States)

A new species of rickettsiae with unknown pathogenicity has been detected in ticks Dermacentor silvarum in the region of Baikal Lake. As revealed by the analysis of the primary structure of the gene fragment coding surface membrane protein of 190 kD (rOmpA), the nucleotide sequence of the rickettsiae under study is mostly similar to the sequences of R. sp. MOAa isolate (96%), R. sp. WB-8-2 (96%), R. massiliae strain GS (94%), Rickettsia BAR-29 (94%), R. rhipicephali (94%). Similarity with the sequence of R. sibirica has proved to be 91%. The data thus obtained indicate that the detected rickettsiae represent a new rickettsial species in the territory of East Siberia. PMID:11871312

Belikov, S M; Digas, S E; Khasnatinov, M A

2001-01-01

197

Phospholipase A and the interaction of Rickettsia prowazekii and mouse fibroblasts (L-929 cells)  

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L-929 cells were killed when approximately 50 viable Rickettsia prowazekii organisms per L-cell were centrifuged onto a monolayer. The glycerophospholipids of the L-cell were hydrolyzed to lysophosphatides and free fatty acids. Concomitantly, there was a loss of membrane integrity as shown by release of lactate dehydrogenase and 86Rb and permeability to trypan blue dye. No glycerophospholipid hydrolysis or cytotoxicity occurred when the rickettsiae were inactivated by heat, UV irradiation, N-ethylmaleimide, or metabolic inhibitors before their addition to the L-929 cells. On the other hand, treatment of the L929 cells with the cytoskeleton agents colchicine or cytochalasin B or with N-ethylmaleimide inhibited neither the phospholipase A activity nor the loss of membrane integrity. Cytochalasin B-treated cells could be damaged by even small numbers of rickettsiae. We suggest that this phospholipase A activity is used by the rickettsiae to escape from the phagosomes into the cytoplasm of host cells.

Winkler, H.H.; Miller, E.T.

1982-10-01

198

Susceptibility of Laboratory Animals to Infection by Spotted Fever Group Rickettsiae.  

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An attempt was made to find suitable laboratory animals as alternative models for the study of the spotted fever group of rickettsiae. Several species, including Mus musculus, Cavia porcellus, Mustelo furo, Meriones unquiculatus, Merocricetus auratus, Syl...

L. S. Sammons R. H. Kenyon R. L. Hickman C. E. Pedersen

1976-01-01

199

Primary isolation of spotted fever group rickettsiae from Amblyomma cooperi collected from Hydrochaeris hydrochaeris in Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english This paper reports the first isolation of a spotted fever group rickettsia from an Amblyomma cooperi ixodid collected from a capybara (Hydrochaeris hydrochaeris) in an endemic area of spotted fever in the County of Pedreira, State of São Paulo, Brazil. Isolation was performed in Vero cell culture an [...] d submitted to immunofluorescence, using antibody from Rickettsia rickettsii-positive human serum.

Elba Regina Sampaio de, Lemos; Heloísa Helena Barbosa, Melles; Sílvia, Colombo; Raimundo Diogo, Machado; José Rodrigues, Coura; Maria Angélica Arpon, Guimarães; Selênio R, Sanseverino; Aline, Moura.

200

Transovarial Transmission of Rickettsia spp. and Organ-Specific Infection of the Whitefly Bemisia tabaci  

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The whitefly Bemisia tabaci is a cosmopolitan insect pest that harbors Portiera aleyrodidarum, the primary obligatory symbiotic bacterium, and several facultative secondary symbionts. Secondary symbionts in B. tabaci are generally associated with the bacteriome, ensuring their vertical transmission; however, Rickettsia is an exception and occupies most of the body cavity, except the bacteriome. The mode of Rickettsia transfer between generations and its subcellular localization in insect orga...

Brumin, Marina; Levy, Maggie; Ghanim, Murad

2012-01-01

 
 
 
 
201

The Rickettsia conorii Autotransporter Protein Sca1 Promotes Adherence to Nonphagocytic Mammalian Cells ? †  

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The pathogenesis of spotted fever group (SFG) Rickettsia species, including R. conorii and R. rickettsii, is acutely dependent on adherence to and invasion of host cells, including cells of the mammalian endothelial system. Bioinformatic analyses of several rickettsia genomes revealed the presence of a cohort of genes designated sca genes that are predicted to encode proteins with homology to autotransporter proteins of Gram-negative bacteria. Previous work demonstrated that three members of ...

2010-01-01

202

Detection of Rickettsia rickettsii antibodies in human sera by crossed immunoelectrophoresis.  

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To identify Rickettsia rickettsii antigens of immunological importance, we examined sera from patients with serologically confirmed cases of Rocky Mountain spotted fever by crossed immunoelectrophoresis for antibodies to antigens extracted from the R strain of R. rickettsii with the detergent Triton X-100. Sixteen antigens were identified in the detergent extract by crossed immunoelectrophoresis with a hyperimmune rabbit serum raised against whole rickettsiae. When the rabbit antiserum was pl...

Anacker, R. L.; Philip, R. N.; Wilfert, C. M.; Kleeman, K. T.; Turner, L.; Maccormack, J. N.; Hechemy, K. E.

1983-01-01

203

Lysis of cells infected with typhus group rickettsiae by a human cytotoxic T cell clone  

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Cytolytic human T cells clones generated in response to the intracellular bacterium Rickettsia typhi were characterized. Growing clones were tested for their ability to proliferate specifically in response to antigens derived from typhus group rickettsiae or to lyse targets infected with R. typhi or Rickettsia prowazekii, as measured by /sup 51/Cr-release from target cells. Two clones were able to lyse targets infected with typhus group rickettsiae. One of these clones was more fully characterized because of its rapid growth characteristics. This cytolytic clone was capable of lysing an autologous infected target as well as a target matched for class I and II histocompatibility leukocyte antigens (HLA). It was not capable, however, of lysing either a target mismatched for both class I and II HLA or a target partially matched for class I HLA. In addition, the clone exhibited specificity in that it was able to lyse an autologous target infected with typhus group rickettsiae, but did not lyse an autologous target infected with an antigenically distinct rickettsial species, Rickettsia tsutsugamushi. These results demonstrate, for the first time, that cells infected with intracellular bacteria can be lysed by human cytotoxic T lymphocytes.

Carl, M.; Robbins, F.; Hartzman, R.J.; Dasch, G.A.

1987-12-15

204

Lysis of cells infected with typhus group rickettsiae by a human cytotoxic T cell clone  

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Cytolytic human T cells clones generated in response to the intracellular bacterium Rickettsia typhi were characterized. Growing clones were tested for their ability to proliferate specifically in response to antigens derived from typhus group rickettsiae or to lyse targets infected with R. typhi or Rickettsia prowazekii, as measured by 51Cr-release from target cells. Two clones were able to lyse targets infected with typhus group rickettsiae. One of these clones was more fully characterized because of its rapid growth characteristics. This cytolytic clone was capable of lysing an autologous infected target as well as a target matched for class I and II histocompatibility leukocyte antigens (HLA). It was not capable, however, of lysing either a target mismatched for both class I and II HLA or a target partially matched for class I HLA. In addition, the clone exhibited specificity in that it was able to lyse an autologous target infected with typhus group rickettsiae, but did not lyse an autologous target infected with an antigenically distinct rickettsial species, Rickettsia tsutsugamushi. These results demonstrate, for the first time, that cells infected with intracellular bacteria can be lysed by human cytotoxic T lymphocytes

1987-12-15

205

Motility characteristics are altered for Rickettsia bellii transformed to overexpress a heterologous rickA gene.  

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The rickettsial protein RickA activates host cell factors associated with the eukaryotic actin cytoskeleton and is likely involved with rickettsial host cell binding and infection and the actin-based motility of spotted fever group rickettsiae. The rickA gene sequence and protein vary substantially between Rickettsia species, as do observed motility-associated phenotypes. To help elucidate the function of RickA and determine the effects of species-specific RickA variations, we compared extracellular binding, intracellular motility, and intercellular spread phenotypes of three Rickettsia bellii variants. These included two shuttle vector-transformed R. bellii strains and the wild-type isolate from which they were derived, R. bellii RML 369C. Both plasmid shuttle vectors carried spectinomycin resistance and a GFPuv reporter; one contained Rickettsia monacensis-derived rickA, and the other lacked the rickA gene. Rickettsia bellii transformed to express R. monacensis rickA highly overexpressed this transcript in comparison to its native rickA. These rickettsiae also moved at higher velocities and followed a more curved path than the negative-control transformants. A lower proportion of R. monacensis rickA-expressing bacteria ever became motile, however, and they formed smaller plaques. PMID:24296498

Oliver, Jonathan D; Burkhardt, Nicole Y; Felsheim, Roderick F; Kurtti, Timothy J; Munderloh, Ulrike G

2014-02-01

206

THE DISTRIBUTION OF RICKETTSIA IN THE TISSUES OF INSECTS AND ARACHNIDS.  

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In the absence of a satisfactory definition of Rickettsia the observations herein recorded were arbitrarily limited to bacterium-like organisms which are intracellular and Gram-negative. Rickettsia of this type were found in the following species: Amblyomma americana, Amblyomma hebraeum, Boophilus decoloratus, Atomus sp., Casinaria infesta, Chrysopa oculata, Ctenocephalus canis, Dermacentor variabilis, Lepisma saccharina, Lucoppia curviseta, Margaropus annulatus, Margaropus annulatus australis, Ornithodoros turicata, Pulex irritans, Rhipicephalus sanguineus, Rhipicephalus evertsi, and Salticus scenicus. Since intracellular, Gram-negative Rickettsia have been recorded in the literature as existing in Cimex lectularius, Dermacentor venustus, Melophagus ovinus, and Pediculus humanus, the occasional occurrence of such bodies must be conceded in the following groups not closely related phylogenetically: Attidae, Trombidiidae, Argasidae, lxodidae, Cinura, Acanthiidae, Pediculidae, Hippoboscidae, Chrysopidae, Pulicidae, and Ichneumonidae. The species which harbor Rickettsia differ widely in diet and habitat. One such species is insectivorous throughout life, two are insectivorous in larval stages, becoming vegetarian in the adult condition, one is chiefly vegetarian but partakes of some animal products, and two are usually entirely vegetarian; while the remainder subsist wholly upon a diet of mammalian blood. Rickettsia are associated, in only a few cases, with diseases in mammals. The evidence at hand does not lead beyond the conclusion that the Rickettsia mentioned above are true Gram-negative microorganisms, easily distinguishable from mitochondria and all other cytoplasmic and nuclear granulations, rather completely adapted to an intracellular existence, exhibiting in some cases a remarkable degree of host specificity, and often inherited through the eggs. PMID:19868737

Cowdry, E V

1923-03-31

207

Identification and localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae).  

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Whiteflies (Homoptera: Aleyrodidae) are sap-sucking insects that harbor "Candidatus Portiera aleyrodidarum," an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. These insects are also home for a diverse facultative microbial community which may include Hamiltonella, Arsenophonus, Fritchea, Wolbachia, and Cardinium spp. In this study, the bacteria associated with a B biotype of the sweet potato whitefly Bemisia tabaci were characterized using molecular fingerprinting techniques, and a Rickettsia sp. was detected for the first time in this insect family. Rickettsia sp. distribution, transmission and localization were studied using PCR and fluorescence in situ hybridizations (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened but not in all individuals within each population. A FISH analysis of B. tabaci eggs, nymphs, and adults revealed a unique concentration of Rickettsia around the gut and follicle cells, as well as a random distribution in the hemolymph. We postulate that the Rickettsia enters the oocyte together with the bacteriocytes, leaves these symbiont-housing cells when the egg is laid, multiplies and spreads throughout the egg during embryogenesis and, subsequently, disperses throughout the body of the hatching nymph, excluding the bacteriomes. Although the role Rickettsia plays in the biology of the whitefly is currently unknown, the vertical transmission on the one hand and the partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others. PMID:16672513

Gottlieb, Yuval; Ghanim, Murad; Chiel, Elad; Gerling, Dan; Portnoy, Vitaly; Steinberg, Shimon; Tzuri, Galil; Horowitz, A Rami; Belausov, Eduard; Mozes-Daube, Neta; Kontsedalov, Svetlana; Gershon, Moshe; Gal, Shunit; Katzir, Nurit; Zchori-Fein, Einat

2006-05-01

208

Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens.  

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Full Text Available The recently sequenced Rickettsia felis genome revealed an unexpected plasmid carrying several genes usually associated with DNA transfer, suggesting that ancestral rickettsiae might have been endowed with a conjugation apparatus. Here we present the genome sequence of Rickettsia bellii, the earliest diverging species of known rickettsiae. The 1,552,076 base pair-long chromosome does not exhibit the colinearity observed between other rickettsia genomes, and encodes a complete set of putative conjugal DNA transfer genes most similar to homologues found in Protochlamydia amoebophila UWE25, an obligate symbiont of amoebae. The genome exhibits many other genes highly similar to homologues in intracellular bacteria of amoebae. We sought and observed sex pili-like cell surface appendages for R. bellii. We also found that R. bellii very efficiently multiplies in the nucleus of eukaryotic cells and survives in the phagocytic amoeba, Acanthamoeba polyphaga. These results suggest that amoeba-like ancestral protozoa could have served as a genetic "melting pot" where the ancestors of rickettsiae and other bacteria promiscuously exchanged genes, eventually leading to their adaptation to the intracellular lifestyle within eukaryotic cells.

2006-05-01

209

Las Rickettsias del grupo de las fiebres manchadas: Respuesta inmune y sus proteínas inmunodominantes Spotted fever Rickettsiae: Their immunodominant proteins and immune response  

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Full Text Available The organisms of Rickettsia species are Gram (- bacteria that cause severe illnesses in humans and are an important health problem in several countries around the world, including Mexico. The presence of different antigens between species and also in different strains of the same specie is an obstacle for vaccine development and serological diagnosis. There are important advances in the knowledge of the rickettsial antigenic structure and the resulting immune response in infected hosts, including humans. This review covers these topics and provides an overview about the development of vaccines and accessible diagnostic methods for diseases caused by Rickettsia (Rev Méd Chile 2004; 132: 381-7.

Jorge Zavala C

2004-03-01

210

Rickettsia felis, an emerging flea-transmitted human pathogen  

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Full Text Available Rickettsia felis was first recognised two decades ago and has now been described as endemic to all continents except Antarctica. The rickettsiosis caused by R. felis is known as flea-borne spotted fever or cat-flea typhus. The large number of arthropod species found to harbour R. felis and that may act as potential vectors support the view that it is a pan-global microbe. The main arthropod reservoir and vector is the cat flea, Ctenocephalides felis, yet more than 20 other species of fleas, ticks, and mites species have been reported to harbour R. felis. Few bacterial pathogens of humans have been found associated with such a diverse range of invertebrates. With the projected increase in global temperature over the next century, there is concern that changes to the ecology and distribution of R. felis vectors may adversely impact public health.

Stephen Graves

2011-07-01

211

Ticks and spotted fever group rickettsiae of southeastern Virginia.  

Science.gov (United States)

The incidence of tick-borne rickettsial disease in the southeastern United States has been rising steadily through the past decade, and the range expansions of tick species and tick-borne infectious agents, new and old, has resulted in an unprecedented mix of vectors and pathogens. The results of an ongoing 4-year surveillance project describe the relative abundance of questing tick populations in southeastern Virginia. Since 2009, more than 66,000 questing ticks of 7 species have been collected from vegetation in a variety of habitats, with Amblyomma americanum constituting over 95% of ticks collected. Other species represented included Ixodes scapularis, Dermacentor variabilis, Amblyomma maculatum, Ixodes affinis, Haemaphysalis leporispalustris, and Ixodes brunneus. We found that 26.9-54.9% of A. americanum ticks tested were positive for Rickettsia amblyommii, a non-pathogenic symbiont of this tick species. We also found no evidence of R. rickettsii in D. variabilis ticks, although they did show low infection rates of R. montanensis (1.5-2.0%). Rickettsia parkeri and Candidatus R. andeanae were found in 41.8-55.7% and 0-1.5% A. maculatum ticks, respectively. The rate of R. parkeri in A. maculatum ticks is among the highest in the literature and has increased in the 2 years since R. parkeri and A. maculatum were first reported in southeastern Virginia. We conclude that tick populations in southeastern Virginia have recently undergone dramatic changes in species and abundance and that these populations support a variety of rickettsial agents with the potential for increased risk to human health. PMID:24201057

Nadolny, Robyn M; Wright, Chelsea L; Sonenshine, Daniel E; Hynes, Wayne L; Gaff, Holly D

2014-02-01

212

Multimethylation of Rickettsia OmpB catalyzed by lysine methyltransferases.  

Science.gov (United States)

Methylation of rickettsial OmpB (outer membrane protein B) has been implicated in bacterial virulence. Rickettsial methyltransferases RP789 and RP027-028 are the first biochemically characterized methyltransferases to catalyze methylation of outer membrane protein (OMP). Methylation in OMP remains poorly understood. Using semiquantitative integrated liquid chromatography-tandem mass spectroscopy, we characterize methylation of (i) recombinantly expressed fragments of Rickettsia typhi OmpB exposed in vitro to trimethyltransferases of Rickettsia prowazekii RP027-028 and of R. typhi RT0101 and to monomethyltransferases of R. prowazekii RP789 and of R. typhi RT0776, and (ii) native OmpBs purified from R. typhi and R. prowazekii strains Breinl, RP22, and Madrid E. We found that in vitro trimethylation occurs at relatively specific locations in OmpB with consensus motifs, KX(G/A/V/I)N and KT(I/L/F), whereas monomethylation is pervasive throughout OmpB. Native OmpB from virulent R. typhi contains mono- and trimethyllysines at locations well correlated with methylation in recombinant OmpB catalyzed by methyltransferases in vitro. Native OmpBs from highly virulent R. prowazekii strains Breinl and RP22 contain multiple clusters of trimethyllysine in contrast to a single cluster in OmpB from mildly virulent R. typhi. Furthermore, OmpB from the avirulent strain Madrid E contains mostly monomethyllysine and no trimethyllysine. The native OmpB from Madrid E was minimally trimethylated by RT0101 or RP027-028, consistent with a processive mechanism of trimethylation. This study provides the first in-depth characterization of methylation of an OMP at the molecular level and may lead to uncovering the link between OmpB methylation and rickettsial virulence. PMID:24497633

Abeykoon, Amila; Wang, Guanghui; Chao, Chien-Chung; Chock, P Boon; Gucek, Marjan; Ching, Wei-Mei; Yang, David C H

2014-03-14

213

Ganglion cells in circumscribed astrocytic tumors: possible implication in classification and prognosis / Implicação da presença de células ganglionares na classificação e evolução de tumores astrocíticos circunscritos  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese INTRODUÇÃO: As neoplasias circunscritas incluem astrocitoma pilocítico (AP), xantoastrocitoma pleomórfico (XP) e ganglioglioma (GG), que compartilham diversas semelhanças, sendo o AP o de melhor prognóstico. Como as células ganglionares (CG) no GG podem ser escassas e os GGs podem recidivar ou evolu [...] ir (grau III), é fundamental o diagnóstico preciso. OBJETIVOS: Identificar CG e corpos granulares eosinofílicos (CGE) em AP e XP, avaliar sua implicação na evolução e comparar com o GG. MÉTODOS: Análise retrospectiva dos aspectos radiológicos, morfológicos e evolutivos (tempo livre de doença, recidiva e óbito) de 30 casos (14 AP, oito XP, oito GG). Cortes corados com hematoxilina e eosina (HE) foram revistos para a identificação da presença de CG neoplásicas e CGE. Estes foram imunomarcados para sinaptofisina (SIN) e neurofilamento (NF) e, em casos selecionados, para glial fibrillary acidic protein (GFAP). RESULTADOS: Seis AP foram reclassificados para GG pela presença de CG (HE ou imunomarcação). Alguns CGE, semelhantes às CG degeneradas, também imunomarcaram para SIN/NF, a maioria sendo negativa para GFAP. O tempo médio livre de doença foi de 62,16 meses. Quatro tumores recidivaram; um deles evoluiu para óbito. Todos os XP possuíam CG, sugerindo que são variantes de GG, dos quais quatro recidivaram (um óbito). O tempo médio livre de doença foi de 69 meses. O aspecto radiológico foi predominantemente cístico. CONCLUSÃO: Sugerimos que AP e XP com CG ou CGE imunopositivos para marcadores neuronais possam ser variantes de GG e alguns CGE representem CG degeneradas; entretanto, a presença de CG ganglionares parece não modificar o comportamento biológico dessas neoplasias. Abstract in english INTRODUCTION: Glial and neuroglial cell neoplasms comprise pilocytic astrocytoma (PA), pleomorphic xanthoastrocytoma (PXA) and ganglioglioma (GG), which share various similarities, though PA has better prognosis. As ganglion cells (GC) may be scarce in GG and these gangliogliomas may recur or progre [...] ss to grade III, an accurate diagnosis is essential. OBJECTIVES: The aim was to identify GC and eosinophilic granular bodies (EGB) in PA and PXA, to evaluate its effect on patient’s outcome and compare them with GG. METHODS: A retrospective analysis of radiological, morphological and follow-up aspects (disease free-survival, recurrence and death) of 30 cases (14 PA, 8 PXA, 8 GG). Hematoxylin and eosin (HE) stained sections were reviewed to identify the presence of neoplastic GC and EGB. They were immunostained for synaptophysin (SYN) and neurofilament (NF). Glial fibrillary acidic protein (GFAP) immunostaining was performed in selected cases. RESULTS: Six PA were reclassified as GG due to the presence of GC by HE or immunohistochemistry. Some EGB resembling degenerate GC were also immunostained for SYN/NF and most of them were negative for GFAP. The mean disease-free survival was 62.16 months. Four tumors recurred and one patient died. All PXA had GC, suggesting that they were variants of GG, 4 of which recurred and one patient died. Mean disease-free survival was 69 months. The radiological aspect was predominantly cystic. CONCLUSION: We propose that PA and PXA with GC or with EGB immunopositive for neuronal markers could be variants of GG, and some EGB may represent degenerate GC. However, the presence of GC does not seem to modify the biological behavior of these neoplasms.

Moreira, Veronica Goulart; Canedo, Nathalie Henriques Silva; Chimelli, Leila Maria Cardão.

214

Índice ganglionar y número de linfonodos metastásicos como factores pronósticos en cáncer de colon Lymph node index as a prognostic factor for survival in stage III colon cancer  

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Full Text Available Introducción: El índice ganglionar (IG se ha propuesto como un factor pronóstico mejor que el número de LN positivos en cáncer de colon estadio III. El objetivo es comparar estos factores en una serie clínica. Pacientes y Método: Se incluyen todos los pacientes estadio III resecados con intención curativa (R0. Se compara la sobrevida según el número de LN positivos y el IG mediante el análisis de las curvas ROC. Resultados: Se trata de 115 pacientes con un promedio de edad de 67,9 años (extremos 25-91, el 63,4% mujeres. El compromiso en profundidad del tumor fue T2 en 3 casos, T3 en 93 casos y T4 en 19. El promedio de ganglios positivos fue 3,4 (extremos 1-34. El índice ganglionar promedio fue 0,237 (DE: 0,197; extremos 0,031-0,882 y la mediana fue 0,1666. El 74% de los pacientes tenía 1 a 3 ganglios positivos (N1 y el 26% 4 o más ganglios positivos (N2. El seguimiento promedio fue de 67 meses (extremos 5-216, durante el cual fallecen 29 pacientes. El área bajo la curva ROC del número de LN afectados (0,703; IC 95%:0,58-082 fue levemente mayor que el área bajo la curva ROC del IG (0,690; IC 95%:0,57-0,81 (p = 0,63. Al compararlas en forma dicotómica, el IG (OR: 19,96; IC 95%:1,51-253,6 muestra una mayor capacidad de discriminación que el número de LN afectados (OR: 2,55; IC 95%: 0,86-7,55. Conclusión: El número de LN metastásicos y el IG son factores pronósticos relevantes en la planificación de la adyuvancia del cáncer de colon estadio III.Background: The lymph node ratio in malignant tumors corresponds to the ratio between the number of involved lymph nodes and the number of examined lymph nodes. This ratio may be a good prognostic index in stage III colon cancer. Aim: To compare the lymph node ratio with the absolute number of positive lymph nodes as prognostic factors in stage III colon cancer. Material and Methods: Analysis of 115 patients aged 25 to 91 years (63% women with a stage III colon cancer operated between 1991 and 2007. Survival according to the absolute number of positive lymph nodes and the lymph node index was calculated. The area under receiver operating characteristic (ROC curves obtained after a COX regression analysis of survival, was used to analyze the prognostic value of each parameter. Results: Lymph node involvement was classified as T2 in three, T3 in 93 and T4 in 19 patients. The mean number of positive lymph nodes was 3.4 (range 1 to 34 and the mean lymph node index was 0.237 ± 0.197 (range 0.031-0.882. Seventy four percent of patients had one to three positive lymph nodes and 24% had more than three. During a mean follow up of 67 months (range 5-216, 29 patients died. In survival analysis, the area under the ROC curve for the number of involved lymph nodes (0.703, 95 confidence intervals (CI 0.58-0.82 was slightly better than the area for lymph node index (0.69, 95% CI 0.57-0.81. Using a dichotomy analysis, a lymph node index over 0.31 had a higher discriminating value for survival (odds ratio (OR 19.96 91% CI 1.51-253.6 than the presence of 12 or more involved lymph nodes (OR 2.55 95% CI 0.86-7.55. Conclusions: The lymph node index and the absolute number of involved lymph nodes are prognostic factors in stage III colon cancer.

GUILLERMO BANNURA C

2011-10-01

215

Complete Genomic DNA Sequence of the East Asian Spotted Fever Disease Agent Rickettsia japonica  

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Rickettsia japonica is an obligate intracellular alphaproteobacteria that causes tick-borne Japanese spotted fever, which has spread throughout East Asia. We determined the complete genomic DNA sequence of R. japonica type strain YH (VR-1363), which consists of 1,283,087 base pairs (bp) and 971 protein-coding genes. Comparison of the genomic DNA sequence of R. japonica with other rickettsiae in the public databases showed that 2 regions (4,323 and 216 bp) were conserved in a very narrow range of Rickettsia species, and the shorter one was inserted in, and disrupted, a preexisting open reading frame (ORF). While it is unknown how the DNA sequences were acquired in R. japonica genomes, it may be a useful signature for the diagnosis of Rickettsia species. Instead of the species-specific inserted DNA sequences, rickettsial genomes contain Rickettsia-specific palindromic elements (RPEs), which are also capable of locating in preexisting ORFs. Precise alignments of protein and DNA sequences involving RPEs showed that when a gene contains an inserted DNA sequence, each rickettsial ortholog carried an inserted DNA sequence at the same locus. The sequence, ATGAC, was shown to be highly frequent and thus characteristic in certain RPEs (RPE-4, RPE-6, and RPE-7). This finding implies that RPE-4, RPE-6, and RPE-7 were derived from a common inserted DNA sequence.

Matsutani, Minenosuke; Ogawa, Motohiko; Takaoka, Naohisa; Hanaoka, Nozomu; Toh, Hidehiro; Yamashita, Atsushi; Oshima, Kenshiro; Hirakawa, Hideki; Kuhara, Satoru; Suzuki, Harumi; Hattori, Masahira; Kishimoto, Toshio; Ando, Shuji; Azuma, Yoshinao; Shirai, Mutsunori

2013-01-01

216

Genotypic identification of three new strains of spotted fever group rickettsiae isolated in China.  

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Polymerase chain reaction (PCR) and restriction endonuclease fragment length polymorphism (RFLP) analysis were used to characterize the genotypic diversity of three isolates of spotted fever group (SFG) rickettsiae isolated from ticks in China. A primer pair designed from DNA sequence encoding 190 K protein antigen of R. rickettsii and genomic DNAs obtained from the isolates were used in PCR. The PCR products were cleaved with restriction endonucleases PstI and RsaI, and the digestion patterns were analyzed by polyacrylamide gel electrophoresis (PAGE) and compared with those of all known species and strains of SFG rickettsiae. The results showed that three isolates had the same PCR products as the other SFG rickettsiae under comparison. HL-93 strain, isolated from Hemophysalis concinna ticks collected in Hulin County, Heilongjiang Province, had unique PstI digestion pattern among SFG rickettsiae; strains BJ-93 and 053, isolated from Dermacentor sinicus and Haemaphysalis concinna ticks collected in Changping County, Beijing City, and Suifenhe City, Heilongjiang Province, respectively, had the same PstI and RsaI digestion patterns as strains R. sibirica 246, BJ-90 and IMTO-85. The present study demonstrated that the BJ-93 and 053 strains were genotypically identical with R. sibirica and the HL-93 strain was genotypically unique among SFG rickettsiae. PMID:9014012

Zhang, J Z; Fan, M Y; Bi, D Z; Cui, W F; Han, Y F

1996-09-01

217

Molecular evidence for a spotted fever group Rickettsia species in the tick Amblyomma longirostre in Brazil.  

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Two Amblyomma longirostre adult male ticks were collected from a Brazilian porcupine Coendou prehensilis L. in the state of Rondonia, Western Amazon, Brazil. The two ticks were pooled for DNA extraction and tested for the presence of rickettsial DNA by amplifying portions of the gltA, 17-kDa, ompA, and ompB rickettsial genes by polymerase chain reaction (PCR). Portions of the four genes were amplified from the sample and subsequently sequenced. These results indicated the presence of a Rickettsia strain infecting A. longirostre, which was designated as strain Aranha. Compared with homologous ompA rickettsial sequences, "Rickettsia amblyommii" ompA seemed to be the closest relative to Aranha (similarity values: 99.0-99.3%). Phylogenetic analyses of more conserved genes including 17-kDa and gtlA partial sequences indicated that this Rickettsia sp. is a spotted fever group rickettsia. The partial ompB sequence of strain Aranha was distinct from all homologous sequences available in GenBank. Although our ompA analysis suggested a very close molecular phylogenetic relationship of Aranha with "R. amblyommii," we cannot at this time determine if Aranha is a new strain of "R. amblyommii" or a new Rickettsia species in South America. PMID:15185961

Labruna, Marcelo B; McBride, Jere W; Bouyer, Donal H; Camargo, Luis Marcelo A; Camargo, Erney P; Walker, David H

2004-05-01

218

Rickettsia 'in' and 'out': two different localization patterns of a bacterial symbiont in the same insect species.  

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Intracellular symbionts of arthropods have diverse influences on their hosts, and their functions generally appear to be associated with their localization within the host. The effect of localization pattern on the role of a particular symbiont cannot normally be tested since the localization pattern within hosts is generally invariant. However, in Israel, the secondary symbiont Rickettsia is unusual in that it presents two distinct localization patterns throughout development and adulthood in its whitefly host, Bemisia tabaci (B biotype). In the "scattered" pattern, Rickettsia is localized throughout the whitefly hemocoel, excluding the bacteriocytes, where the obligate symbiont Portiera aleyrodidarum and some other secondary symbionts are housed. In the "confined" pattern, Rickettsia is restricted to the bacteriocytes. We examined the effects of these patterns on Rickettsia densities, association with other symbionts (Portiera and Hamiltonella defensa inside the bacteriocytes) and on the potential for horizontal transmission to the parasitoid wasp, Eretmocerus mundus, while the wasp larvae are developing within the whitefly nymph. Sequences of four Rickettsia genes were found to be identical for both localization patterns, suggesting that they are closely related strains. However, real-time PCR analysis showed very different dynamics for the two localization types. On the first day post-adult emergence, Rickettsia densities were 21 times higher in the "confined" pattern vs. "scattered" pattern whiteflies. During adulthood, Rickettsia increased in density in the "scattered" pattern whiteflies until it reached the "confined" pattern Rickettsia density on day 21. No correlation between Rickettsia densities and Hamiltonella or Portiera densities were found for either localization pattern. Using FISH technique, we found Rickettsia in the gut of the parasitoid wasps only when they developed on whiteflies with the "scattered" pattern. The results suggest that the localization pattern of a symbiont may influence its dynamics within the host. PMID:21712994

Caspi-Fluger, Ayelet; Inbar, Moshe; Mozes-Daube, Netta; Mouton, Laurence; Hunter, Martha S; Zchori-Fein, Einat

2011-01-01

219

Rickettsia, Ehrlichia, Anaplasma, and Bartonella in ticks and fleas from dogs and cats in Bangkok.  

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Flea and tick specimens (5-10 fleas or ticks) on dogs and cats from various sites in Bangkok were tested by polymerase chain reaction and DNA sequencing to detect DNA of bacteria Rickettsia (gltA and 17?kDa genes), Anaplasmataceae (16S rRNA gene), and Bartonella (pap31 and its genes). We confirmed that Rickettsia sp. related to Rickettsia felis was detected in 66 of 98 (67.4%) flea specimens from dogs, whereas 8 Bartonella henselae and 2 Bartonella clarridgeiae were detected in 10 of 54 (18.5%) flea specimens from cats. Further, this work provides the first evidence of 10 Ehrlichia canis (3.3%), 7 Anaplasma platys (2.3%), and 2 Wolbachia spp. (0.66%) in 304 Rhipicephalus sanguineus tick specimens in Thailand. PMID:21612535

Foongladda, Suporn; Inthawong, Dutsadee; Kositanont, Uraiwan; Gaywee, Jariyanart

2011-10-01

220

Rickettsia africae in Hyalomma dromedarii ticks from sub-Saharan Algeria.  

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Spotted fever group (SFG) rickettsioses are caused by obligate, intracellular Gram-negative bacteria of the genus Rickettsia. In recent years, several species and subspecies of rickettsias have been identified as emerging pathogens throughout the world, including sub-Saharan Africa. We report here the detection of Rickettsia africae, the agent responsible for African tick-bite fever, by amplification of fragments of gltA and ompA genes and multi-spacer typing from Hyalomma dromedarii ticks collected from the camel Camelus dromedarius in the Adrar and Béchar region (sub-Saharan Algeria). To date, R. africae has been associated mainly with Amblyomma spp. The role of H. dromedarii in the epidemiology of R. africae requires further investigation. PMID:23164496

Kernif, Tahar; Djerbouh, Amel; Mediannikov, Oleg; Ayach, Bouhous; Rolain, Jean-Marc; Raoult, Didier; Parola, Philippe; Bitam, Idir

2012-12-01

 
 
 
 
221

Common epidemiology of Rickettsia felis infection and malaria, Africa.  

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This study aimed to compare the epidemiology of Rickettsia felis infection and malaria in France, North Africa, and sub-Saharan Africa and to identify a common vector. Blood specimens from 3,122 febrile patients and from 500 nonfebrile persons were analyzed for R. felis and Plasmodium spp. We observed a significant linear trend (p<0.0001) of increasing risk for R. felis infection. The risks were lowest in France, Tunisia, and Algeria (1%), and highest in rural Senegal (15%). Co-infections with R. felis and Plasmodium spp. and occurrences of R. felis relapses or reinfections were identified. This study demonstrates a correlation between malaria and R. felis infection regarding geographic distribution, seasonality, asymptomatic infections, and a potential vector. R. felis infection should be suspected in these geographical areas where malaria is endemic. Doxycycline chemoprophylaxis against malaria in travelers to sub-Saharan Africa also protects against rickettsioses; thus, empirical treatment strategies for febrile illness for travelers and residents in sub-Saharan Africa may require reevaluation. PMID:24188709

Mediannikov, Oleg; Socolovschi, Cristina; Edouard, Sophie; Fenollar, Florence; Mouffok, Nadjet; Bassene, Hubert; Diatta, Georges; Tall, Adama; Niangaly, Hamidou; Doumbo, Ogobara; Lekana-Douki, Jean Bernard; Znazen, Abir; Sarih, M'hammed; Ratmanov, Pavel; Richet, Herve; Ndiath, Mamadou O; Sokhna, Cheikh; Parola, Philippe; Raoult, Didier

2013-11-01

222

Characterization and Growth of Polymorphic Rickettsia felis in a Tick Cell Line?  

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Morphological differentiation in some arthropod-borne bacteria is correlated with increased bacterial virulence, transmission potential, and/or as a response to environmental stress. In the current study, we utilized an in vitro model to examine Rickettsia felis morphology and growth under various culture conditions and bacterial densities to identify potential factors that contribute to polymorphism in rickettsiae. We utilized microscopy (electron microscopy and immunofluorescence), genomic (PCR amplification and DNA sequencing of rickettsial genes), and proteomic (Western blotting and liquid chromatography-tandem mass spectrometry) techniques to identify and characterize morphologically distinct, long-form R. felis. Without exchange of host cell growth medium, polymorphic R. felis was detected at 12 days postinoculation when rickettsiae were seeded at a multiplicity of infection (MOI) of 5 and 50. Compared to short-form R. felis organisms, no change in membrane ultrastructure in long-form polymorphic rickettsiae was observed, and rickettsiae were up to six times the length of typical short-form rickettsiae. In vitro assays demonstrated that short-form R. felis entered into and replicated in host cells faster than long-form R. felis. However, when both short- and long-form R. felis organisms were maintained in cell-free medium for 12 days, the infectivity of short-form R. felis was decreased compared to long-form R. felis organisms, which were capable of entering host cells, suggesting that long-form R. felis is more stable outside the host cell. The relationship between rickettsial polymorphism and rickettsial survivorship should be examined further as the yet undetermined route of horizontal transmission of R. felis may utilize metabolically and morphologically distinct forms for successful transmission.

Sunyakumthorn, Piyanate; Bourchookarn, Apichai; Pornwiroon, Walairat; David, Connie; Barker, Steven A.; Macaluso, Kevin R.

2008-01-01

223

Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia  

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Full Text Available Abstract The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and gltA genes were amplified from the blood of 3 dogs. This study is the first report of R. felis infection in indigenous community dogs in NT.

Rees Robert L

2011-10-01

224

The seroprevalence of human infection with Rickettsia slovaca, in an area of northern Spain  

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An epidemiological survey was undertaken to explore human exposure to Rickettsia slovaca in two provinces of northern Spain. When IFAT were used to test 200 members of the general population for antibodies to rickettsiae of the spotted-fever group, six (3.3%) were found positive, presumably, since Dermacentor is one of the most common genera of human-biting tick in the study area, for antibodies to R. slovaca. Thirty-one (16.9%) of an additional 183 subjects who presented shortly after being ...

Lledo?, L.; Gegu?ndez, M. I.; Fernandes, N.; Sousa, R.; Vicente, J.; A?lamo Sanz, Rufino; Ferna?ndez Soto, Pedro; Pe?rez Sa?nchez, Ricardo; Bacellar, F.

2006-01-01

225

Occurrence of rickettsia-like symbionts among species of the Aedes scutellaris group (Diptera:Culicidae).  

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Rickettsia-like organisms were seen by electron microscopy in the oocytes and nurse cells of Aedes hebrideus and A. scutellaris scutellaris and in the oocytes of A. pseudoscutellaris, a Samoan stock of A. polynesiensis and in three Fijian stocks of A. polynesiensis. None were found in the ovaries of A. alcasidi, A. s. katherinensis, A. cooki nor in a second stock of Samoan A. polynesiensis. It is suggested that the distribution of rickettsia-like symbionts among species of the A. scutellaris group conflicts in some cases with the hypothesis that these organisms are responsible for cytoplasmic incompatibility in the group. PMID:6433816

Meek, S R

1984-08-01

226

Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia  

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The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT) was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG)-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and gltA genes were amplified from the blood of 3 dogs. This study is ...

2011-01-01

227

Identification of Rickettsia felis in fleas but not ticks on stray cats and dogs and the evidence of Rickettsia rhipicephali only in adult stage of Rhipicephalus sanguineus and Rhipicephalus haemaphysaloides.  

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Rickettsia spp. are zoonotic pathogens and mainly transmitted by various arthropod vectors, such as fleas, ticks, and lice. Previous epidemiological studies indicated that ectoparasites infested on dogs or cats may be infected by Rickettsia spp., and transmit them to human beings accidentally. In this study, the prevalence of Rickettsia infection was evaluated using fleas and ticks from stray dogs and cats in Taiwan. A total of 158 pools made by 451 cat fleas (Ctenocephalides felis) from 37 dogs and 4 cats were used for analysis. Besides, 386 Rhipicephalus ticks collected from the other 62 stray dogs were included in this study. Nymphal and adult ticks were individually analyzed but larvae were separated into 21 pools for molecular detection. Partial sequencing analysis of the gltA gene was applied for Rickettsia identification. The results showed that 44.3% (70/158) of the cat flea pools were harboring Rickettsia DNA. Although 6.9% (13/187) of adult ticks were infected with Rickettsia, neither larval pools nor nymphal ticks were found to contain Rickettsia DNA. According to the results of sequencing analyses, all Rickettsia PCR-positive cat flea pools were infected with R. felis, and all Rickettsia PCR-positive adult ticks were infected with R. rhipicephali. The results of this study demonstrated that C. felis but not Rhipicephlus sanguineus (the brown dog tick) and Rh. haemaphysaloides collected from stray animals in Taiwan could be infected the zoonotic pathogen R. felis. Moreover, R. rhipicephali was only identified in adult stage of Rhipicephalus sanguineus and Rh. haemaphysaloides. PMID:22000945

Hsu, Yuan-Man; Lin, Chao-Chen; Chomel, Bruno B; Tsai, Kun-Hsien; Wu, Wen-Jer; Huang, Chin-Gi; Chang, Chao-Chin

2011-12-01

228

Evidence of spotted fever group rickettsiae in state of Rio de Janeiro, Brazil Evidência de rickettsiae do grupo da febre maculosa no Estado do Rio de Janeiro, Brasil  

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Full Text Available Ticks were obtained from dogs from February to September of 1999 at weekly intervals, in the County of Piraí, State of Rio de Janeiro. Four hundred seventy four ixodids were taxonomically identified, 103 Amblyomma cajennense, seven Amblyomma ovale, 209 Rhipicephalus sanguineus, and 155 Amblyomma sp. An hemolymph test associated with Giemsa's stain revealed two specimens in 163 ticks tested (R. sanguineus and Amblyomma sp, containing rickettsia-like organisms. Direct immunofluorescence verified the presence of spotted fever group rickettsia in one specimen of R. sanguineus. Considering the limited information on rickettsiosis in Brazil, principally in relation to the vectors involved in perpetuating it in foci, these preliminary results give us an idea on the importance of infection in ticks, allowing to expand our knowledge on this zoonosis.De fevereiro a setembro de 1999, foram realizadas, semanalmente, coletas de carrapatos de cães no Município de Piraí/RJ. Quatrocentos e setenta e quatro ixodídeos foram taxonomicamente identificados, 103 Amblyomma cajennense, sete Amblyomma ovale, 209 Rhipicephalus sanguineus e 155 Amblyomma sp. O teste de hemolinfa associado à coloração de Giemsa revelou que duas espécies de 163 carrapatos testados (R. sanguineus e A. sp. continham microrganismos com morfologia semelhante à rickettsia do grupo da febre maculosa. No teste de imunofluorescência direta, mais específico, foi verificada a presença de rickettsia do grupo da febre maculosa em uma espécie de R. sanguineus. Considerando que informações sobre rickettsioses no Brasil são limitadas, principalmente com relação aos vetores envolvidos na perpetuação da doença, estes resultados preliminares nos mostram a necessidade da realização deste tipo de estudo, permitindo, desta forma, aumentar nossos conhecimentos a respeito desta zoonose.

Tatiana ROZENTAL

2002-01-01

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Detecção de riquétsias em carrapatos do gênero Amblyomma (Acari: Ixodidae) coletados em parque urbano do município de Campinas, SP / Rickettsiae detection in Amblyomma ticks (Acari: Ixodidae) collected in the urban area of Campinas city, SP  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O Município de Campinas situa-se em região endêmica para febre maculosa brasileira do Estado de São Paulo, onde vários casos desta doença vem ocorrendo. Capivaras têm sido associadas ao ciclo dessa riquetsiose por apresentarem sorologia positiva e serem hospedeiras de carrapatos Amblyomma spp princi [...] pais vetores da doença. Carrapatos foram coletados no parque urbano do Lago do Café, Campinas, SP, local associado a casos humanos suspeitos de febre maculosa brasileira, sobre a vegetação e das capivaras ali presentes, e pesquisados quanto à presença de riquétsias pela reação em cadeia da polimerase e pelo teste de hemolinfa. Adultos de Amblyomma cajennense e Amblyomma cooperi albergavam Rickettsia bellii, não patogênica, identificada pela análise das seqüências de nucleotídeos do gene gltA, porém, não foram constatadas riquétsias do Grupo da Febre Maculosa. Estes resultados associados à ausência de um isolado de riquétsias do Grupo da Febre Maculosa de capivaras indicam que seu papel, enquanto reservatório, necessita de maior investigação. Abstract in english The city of Campinas is located in an endemic area for brazilian spotted fever in São Paulo State, where several cases have recently occurred. Capybaras have been associated with the cycle of this disease, for they present positive serology and serve as host for ticks of the genus Amblyomma, the mai [...] n vectors of brazilian spotted fever. Ticks were colleted both from Capybaras and from the vegetation in the city park Lago do Café, located in the urban area of Campinas city, SP, a site associated with suspected human cases of brazilian spotted fever. The ticks collected were examinaded for the presence of rickettsiae using polymerase chain reaction and the haemolymph test. Through analysis of the gene gltA nucleotide sequence, adults of Amblyomma cajennense and Amblyomma cooperi were found to be infected with the non pathogenic Rickettsia bellii. However, no rickettsiae of the spotted fever group were detected. These results indicate that the role of capybaras as reservoirs of rickettsiae of the Spotted Fever group is still uncertain and further studies are required.

Estrada, Dora Amparo; Schumaker, Teresinha Tizu Sato; Souza, Celso Eduardo de; Rodrigues Neto, Elias José; Linhares, Arício Xavier.

230

Índice ganglionar y número de linfonodos metastásicos como factores pronósticos en cáncer de colon / Lymph node index as a prognostic factor for survival in stage III colon cancer  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: El índice ganglionar (IG) se ha propuesto como un factor pronóstico mejor que el número de LN positivos en cáncer de colon estadio III. El objetivo es comparar estos factores en una serie clínica. Pacientes y Método: Se incluyen todos los pacientes estadio III resecados con intención c [...] urativa (R0). Se compara la sobrevida según el número de LN positivos y el IG mediante el análisis de las curvas ROC. Resultados: Se trata de 115 pacientes con un promedio de edad de 67,9 años (extremos 25-91), el 63,4% mujeres. El compromiso en profundidad del tumor fue T2 en 3 casos, T3 en 93 casos y T4 en 19. El promedio de ganglios positivos fue 3,4 (extremos 1-34). El índice ganglionar promedio fue 0,237 (DE: 0,197; extremos 0,031-0,882) y la mediana fue 0,1666. El 74% de los pacientes tenía 1 a 3 ganglios positivos (N1) y el 26% 4 o más ganglios positivos (N2). El seguimiento promedio fue de 67 meses (extremos 5-216), durante el cual fallecen 29 pacientes. El área bajo la curva ROC del número de LN afectados (0,703; IC 95%:0,58-082) fue levemente mayor que el área bajo la curva ROC del IG (0,690; IC 95%:0,57-0,81) (p = 0,63). Al compararlas en forma dicotómica, el IG (OR: 19,96; IC 95%:1,51-253,6) muestra una mayor capacidad de discriminación que el número de LN afectados (OR: 2,55; IC 95%: 0,86-7,55). Conclusión: El número de LN metastásicos y el IG son factores pronósticos relevantes en la planificación de la adyuvancia del cáncer de colon estadio III. Abstract in english Background: The lymph node ratio in malignant tumors corresponds to the ratio between the number of involved lymph nodes and the number of examined lymph nodes. This ratio may be a good prognostic index in stage III colon cancer. Aim: To compare the lymph node ratio with the absolute number of posit [...] ive lymph nodes as prognostic factors in stage III colon cancer. Material and Methods: Analysis of 115 patients aged 25 to 91 years (63% women) with a stage III colon cancer operated between 1991 and 2007. Survival according to the absolute number of positive lymph nodes and the lymph node index was calculated. The area under receiver operating characteristic (ROC) curves obtained after a COX regression analysis of survival, was used to analyze the prognostic value of each parameter. Results: Lymph node involvement was classified as T2 in three, T3 in 93 and T4 in 19 patients. The mean number of positive lymph nodes was 3.4 (range 1 to 34) and the mean lymph node index was 0.237 ± 0.197 (range 0.031-0.882). Seventy four percent of patients had one to three positive lymph nodes and 24% had more than three. During a mean follow up of 67 months (range 5-216), 29 patients died. In survival analysis, the area under the ROC curve for the number of involved lymph nodes (0.703, 95 confidence intervals (CI) 0.58-0.82) was slightly better than the area for lymph node index (0.69, 95% CI 0.57-0.81). Using a dichotomy analysis, a lymph node index over 0.31 had a higher discriminating value for survival (odds ratio (OR) 19.96 91% CI 1.51-253.6) than the presence of 12 or more involved lymph nodes (OR 2.55 95% CI 0.86-7.55). Conclusions: The lymph node index and the absolute number of involved lymph nodes are prognostic factors in stage III colon cancer.

GUILLERMO, BANNURA C; CLAUDIO, VARGAS S; ALEJANDRO, BARRERA E; CARLOS, MELO L; JAIME, CONTRERAS P.

231

Comparison of a latex agglutination procedure with the microimmunofluorescence test for Rickettsia typhi.  

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Sera submitted to the Texas Department of Health for the serodiagnosis of Rickettsia typhi were tested by the microimmunofluorescent antibody technique and a new latex agglutination procedure. Results indicated that the latex agglutination test was sensitive and specific and would serve well as a first-line screening test for murine typhus.

Rawlings, J. A.; Elliott, L. B.; Little, L. M.

1985-01-01

232

Rickettsiae and ehrlichiae within a city park: is the urban dweller at risk?  

Science.gov (United States)

Tick-borne diseases, such as spotted fever rickettsioses and ehrlichioses, are potentially severe and life-threatening infections. The incidences of these infections increase during warm weather months as ticks become active. Clinicians often consider outdoor activities in rural areas to be a risk factor for exposure to ticks and the pathogens they carry, but are those who live, work, and play within an urban environment excluded from this risk? In this study, we collected ticks from two urban parks in Little Rock, AR, to assess the presence of rickettsiae and ehrlichiae within an urban setting. A total of 273 ticks were collected during July, 2011. Amblyomma americanum was the predominant tick species, with 255 (93%) of those collected. The remaining 18 (7%) were Dermacentor variabilis. Ticks were separated and pooled into groups for further testing. Forty-two of the 43 (98%) A. americanum pools demonstrated molecular evidence for the presence of rickettsiae. None of the D. variabilis contained rickettsiae. Restriction enzyme fragment length polymorphism analysis and DNA sequencing revealed Rickettsia amblyommii to be the species present. One A. americanum pool from park A demonstrated the presence of Ehrlichia chaffeensis, the pathogen responsible for human monocytotropic ehrlichiosis. These results indicate that tick-borne pathogens are not limited to rural or suburban areas. PMID:24528097

Blanton, Lucas S; Walker, David H; Bouyer, Donald H

2014-02-01

233

Rickettsia infection in five areas of the state of São Paulo, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus [...] , Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi), and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus) were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii), some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.

Horta, Maurício C; Labruna, Marcelo B; Pinter, Adriano; Linardi, Pedro M; Schumaker, Teresinha T S.

234

Rickettsia infection in five areas of the state of São Paulo, Brazil  

Directory of Open Access Journals (Sweden)

Full Text Available This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus, Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi, and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii, some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.

Maurício C Horta

2007-11-01

235

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples  

DEFF Research Database (Denmark)

A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

Svendsen, Claus Bo; Boye, Mette

2009-01-01

236

Rickettsia slovaca in Dermacentor marginatus ticks and Tick Borne Lymphadenopathy in Tuscany, central Italy  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Of 263 patients in Tuscany, Italy, from whom ticks were removed during July 2005–May 2007, fi ve showed signs of tick-borne lymphadenopathy. Of the ticks, 17 were Dermacentor marginatus; 6 (35.3%) of these were identifi ed by sequence analysis as containing Rickettsia slovaca. Tickborne lymphadenopathy occurs in this area.

Mannelli, Alessandro; Tomassone, Laura; Bertolotti, Luigi

2008-01-01

237

The Rickettsia conorii Autotransporter Protein Sca1 Promotes Adherence to Nonphagocytic Mammalian Cells ? †  

Science.gov (United States)

The pathogenesis of spotted fever group (SFG) Rickettsia species, including R. conorii and R. rickettsii, is acutely dependent on adherence to and invasion of host cells, including cells of the mammalian endothelial system. Bioinformatic analyses of several rickettsia genomes revealed the presence of a cohort of genes designated sca genes that are predicted to encode proteins with homology to autotransporter proteins of Gram-negative bacteria. Previous work demonstrated that three members of this family, rOmpA (Sca0), Sca2, and rOmpB (Sca5) are involved in the interaction with mammalian cells; however, very little was known about the function of other conserved rickettsial Sca proteins. Here we demonstrate that sca1, a gene present in nearly all SFG rickettsia genomes, is actively transcribed and expressed in R. conorii cells. Alignment of Sca1 sequences from geographically diverse SFG Rickettsia species showed that there are high degrees of sequence identity and conservation of these sequences, suggesting that Sca1 may have a conserved function. Using a heterologous expression system, we demonstrated that production of R. conorii Sca1 in the Escherichia coli outer membrane is sufficient to mediate attachment to but not invasion of a panel of cultured mammalian epithelial and endothelial cells. Furthermore, preincubation of a recombinant Sca1 peptide with host cells blocked R. conorii cell association. Together, these results demonstrate that attachment to mammalian cells can be uncoupled from the entry process and that Sca1 is involved in the adherence of R. conorii to host cells.

Riley, Sean P.; Goh, Kenneth C.; Hermanas, Timothy M.; Cardwell, Marissa M.; Chan, Yvonne G. Y.; Martinez, Juan J.

2010-01-01

238

Detection of Point Mutations in rpoB Gene of Rifampin-Resistant Rickettsia typhi  

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The rpoB gene of rifampin-resistant Rickettsia typhi (Rif mutant) and wild-type R. typhi were sequenced and compared. The Rif mutant rpoB had three nucleotide substitutions, which resulted in amino acid changes at residues 151, 201, and 271 and may be the basis for the rifampin resistance.

Troyer, Jill Michelle; Radulovic, Suzana; Andersson, Siv G. E.; Azad, Abdu F.

1998-01-01

239

Molecular detection and characterization of spotted fever group rickettsiae in Taiwan.  

Science.gov (United States)

Rickettsioses are emerging infectious diseases caused by rickettsiae in association with arthropods. We report the detection of spotted fever group rickettsiae (SFGR) in Taiwan using molecular methods. Phylogenetic analyses of the 17-kd protein and citrate synthase (gltA) genes showed that SFGR TwKM01 detected in Rhipicephalus haemaphysaloides ticks was most similar to Rickettsia rhipicephali. Three TwKM01 isolates were obtained from three individual R. haemaphysaloides ticks. Small, intracellular, coccobacillary bacteria were found in infected L929 cells using immunofluorescence antibody testing and transmission electron microscopy. Two other SFGRs, TwKM02 and TwKM03, identified in Leptotrombidium chigger mites, were closely related to R. australis and R. felis URRWXCal(2), respectively. The TwKM03 strain was also detected in Ixodes granulatus ticks and widely distributed in Hualien, Kinmen, and Lienchiang counties in Taiwan. The endonucleases MaeII and HhaI selected for restriction fragment length polymorphism analysis of the gltA and 17-kd polymerase chain reaction products, respectively, were useful for genotyping Rickettsia species TwKM01, TwKM02, TwKM03, and other SFGRs. Although their infectivity and pathogenicity for vertebrates are unknown, the finding of SFGRs raises the possibility that bacteria other than Orientia tsutsugamushi, Coxiella burnetii, and R. typhi may be involved in rickettsial diseases in Taiwan. PMID:17984347

Tsui, Pei-Yi; Tsai, Kun-Hsien; Weng, Ming-Hui; Hung, Yao-Wen; Liu, Yu-Tien; Hu, Kuang-Yu; Lien, Jih-Ching; Lin, Pey-Ru; Shaio, Men-Fang; Wang, Hsi-Chieh; Wang, Hsis-Chieh; Ji, Dar-Der

2007-11-01

240

Pesquisa de Rickettsia spp em carrapatos Amblyomma cajennense e Amblyomma dubitatum no Estado de São Paulo / Survey of Rickettsia spp in the ticks Amblyomma cajennense and Amblyomma dubitatum in the State of São Paulo  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Foi pesquisada a presença de riquétsias em 3.545 carrapatos Amblyomma cajennense e 2.666 Amblyomma dubitatum. Através do teste de hemolinfa, reação em cadeia pela polimerase e isolamento de rickettsia em cultivo celular, todos os Amblyomma cajennense foram negativos, sendo que 634 (23,8%) Amblyomma [...] dubitatum mostraram-se infectados com Rickettsia bellii. Abstract in english The presence of rickettsial infection was surveyed in 3,545 Amblyomma cajennense ticks and 2,666 Amblyomma dubitatum ticks. Using the hemolymph test, polymerase chain reaction and isolation of Rickettsia in cell cultures, all of the Amblyomma cajennense were negative, whereas 634 (23.8%) of the Ambl [...] yomma dubitatum ticks were shown to be infected with Rickettsia bellii.

Pacheco, Richard Campos; Horta, Maurício Cláudio; Pinter, Adriano; Moraes-Filho, Jonas; Martins, Thiago Fernandes; Nardi, Marcello Schiavo; Souza, Savina Silvana Aparecida Lacerra de; Souza, Celso Eduardo de; Szabó, Matias Pablo Juan; Richtzenhain, Leonardo José; Labruna, Marcelo Bahia.

 
 
 
 
241

Pesquisa de Rickettsia spp em carrapatos Amblyomma cajennense e Amblyomma dubitatum no Estado de São Paulo Survey of Rickettsia spp in the ticks Amblyomma cajennense and Amblyomma dubitatum in the State of São Paulo  

Directory of Open Access Journals (Sweden)

Full Text Available Foi pesquisada a presença de riquétsias em 3.545 carrapatos Amblyomma cajennense e 2.666 Amblyomma dubitatum. Através do teste de hemolinfa, reação em cadeia pela polimerase e isolamento de rickettsia em cultivo celular, todos os Amblyomma cajennense foram negativos, sendo que 634 (23,8% Amblyomma dubitatum mostraram-se infectados com Rickettsia bellii.The presence of rickettsial infection was surveyed in 3,545 Amblyomma cajennense ticks and 2,666 Amblyomma dubitatum ticks. Using the hemolymph test, polymerase chain reaction and isolation of Rickettsia in cell cultures, all of the Amblyomma cajennense were negative, whereas 634 (23.8% of the Amblyomma dubitatum ticks were shown to be infected with Rickettsia bellii.

Richard Campos Pacheco

2009-06-01

242

Serosurvey of antibodies against spotted fever group Rickettsia spp. in horse farms in Northern Paraná, Brazil Soroprevalência de anticorpos contra Rickettsia spp. do grupo febre maculosa em equinos de haras no Norte do Paraná, Brasil  

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Brazilian spotted fever (BSF) is an emerging disease most likely caused by Rickettsia rickettsii. The objective of the present study was to estimate the seroprevalence of BSF rickettsia infections in equines from six horse farms located in Londrina County, Paraná, Southern Brazil. Six owners of horse farms situated in Cambé, Santa Fé, Guaraci and Londrina municipalities participated in the study. All farms were located in areas where BSF has not been reported. A total of 273 horses were sa...

2010-01-01

243

Molecular detection of Rickettsia felis and Bartonella henselae in dog and cat fleas in Central Oromia, Ethiopia.  

Science.gov (United States)

Fleas are important vectors of several Rickettsia and Bartonella spp. that cause emerging zoonotic diseases worldwide. In this study, 303 fleas collected from domestic dogs and cats in Ethiopia and identified morphologically as Ctenocephalides felis felis, C. canis, Pulex irritans, and Echidnophaga gallinacea were tested for Rickettsia and Bartonella DNA by using molecular methods. Rickettsia felis was detected in 21% of fleas, primarily C. felis, with a similar prevalence in fleas from dogs and cats. A larger proportion of flea-infested dogs (69%) than cats (37%) harbored at least one C. felis infected with R. felis. Rickettsia typhi was not detected. Bartonella henselae DNA was detected in 6% (2 of 34) of C. felis collected from cats. Our study highlights the likelihood of human exposure to R. felis, an emerging agent of spotted fever, and B. henselae, the agent of cat-scratch disease, in urban areas in Ethiopia. PMID:24445204

Kumsa, Bersissa; Parola, Philippe; Raoult, Didier; Socolovschi, Cristina

2014-03-01

244

Rickettsia in synanthropic and domestic animals and their hosts from two areas of low endemicity for Brazilian spotted fever in the eastern region of Minas Gerais, Brazil.  

Science.gov (United States)

The aim of this study was to understand the current epidemiology of rickettsial diseases in two rickettsial-endemic regions in Brazil. In the municipalities of Pingo D'Agua and Santa Cruz do Escalvado, among serum samples obtained from horses and dogs, reactivity by immunofluorescent assay against spotted fever group rickettsiae was verified. In some serum samples from opossums (Didelphis aurita) captured in Santa Cruz do Escalvado, serologic response against rickettsiae was also verified. Polymerase chain reaction identified rickettsiae only in ticks and fleas obtained in Santa Cruz do Escalvado. Rickettsiae in samples had 100% sequence homology with Rickettsia felis. These results highlight the importance of marsupials in maintenance of the sylvatic cycle of rickettsial disease and potential integration with the domestic cycle. Our data also support the importance of horses and dogs as sentinels in monitoring circulation of rickettsiae in an urban area. PMID:21118939

Milagres, Bruno S; Padilha, Amanda F; Barcelos, Rafael M; Gomes, Gabriel G; Montandon, Carlos E; Pena, Dárlen C H; Nieri Bastos, Fernanda A; Silveira, Iara; Pacheco, Richard; Labruna, Marcelo B; Bouyer, Donald H; Freitas, Renata N; Walker, David H; Mafra, Cláudio L; Galvao, Márcio A M

2010-12-01

245

New Spotted Fever Group Rickettsia in a Rhipicephalus turanicus Tick Removed from a Child in Eastern Sicily, Italy  

Digital Repository Infrastructure Vision for European Research (DRIVER)

A new genotype of spotted fever group Rickettsia (SFGR) was identified in Rhipicephalus turanicus from eastern Sicily. On the basis of current molecular criteria, the genetic characteristics obtained from multiple locus sequence typing satisfy the requirements for Candidatus status of this SFGR. Further detection and identification of this SFGR during entomological and clinical surveys will be required to establish the prevalence of this Rickettsia and its potential pathogenicity for humans.

Eremeeva, Marina E.; Stromdahl, Ellen Y.

2011-01-01

246

Cloning, sequencing, and expression of the gene coding for an antigenic 120-kilodalton protein of Rickettsia conorii.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Several high-molecular-mass (above 100 kDa) antigens are recognized by sera from humans infected with spotted fever group rickettsiae and may be important stimulators of the host immune response. Molecular cloning techniques were used to make genomic Rickettsia conorii (Malish 7 strain) libraries in expression vector lambda gt11. The 120-kDa R. conorii antigen was identified by monospecific antibodies to the recombinant protein expressed on construct lambda 4-7. The entire gene DNA sequence w...

Schuenke, K. W.; Walker, D. H.

1994-01-01

247

First Isolation and Identification of Rickettsia conorii from Ticks Collected in the Region of Fokida in Central Greece  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Three different spotted-fever group rickettsiae—Rickettsia conorii, R. massiliae, and R. rhipicephali—were detected and identified by PCR-restriction fragment length polymorphism analysis in Rhipicephalus ticks collected from domestic animals in the Fokida region of Greece, where a high seroprevalence of antibodies to R. conorii was previously demonstrated. The infection rate of ticks was 1.6%. Moreover, R. conorii was isolated from one Rhipicephalus sanguineus tick.

2003-01-01

248

Penetration of host cells by Rickettsia rickettsii appears to be mediated by a phospholipase of rickettsial origin.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Internalization of obligate intracellular bacteria belonging to the genus Rickettsia by eukaryotic cells requires participation of both the parasitized host and the microorganism. The term "induced phagocytosis" has been used specifically to describe the entry of Rickettsia prowazekii, although a similar mechanism is likely for R. rickettsii. A role for a phospholipase in the internalization process has been proposed for both of these organisms, with the strongest supporting evidence ...

1992-01-01

249

Rickettsia Symbiont in the Pea Aphid Acyrthosiphon pisum: Novel Cellular Tropism, Effect on Host Fitness, and Interaction with the Essential Symbiont Buchnera  

Digital Repository Infrastructure Vision for European Research (DRIVER)

In natural populations of the pea aphid Acyrthosiphon pisum, a facultative bacterial symbiont of the genus Rickettsia has been detected at considerable infection frequencies worldwide. We investigated the effects of the Rickettsia symbiont on the host aphid and also on the coexisting essential symbiont Buchnera. In situ hybridization revealed that the Rickettsia symbiont was specifically localized in two types of host cells specialized for endosymbiosis: secondary mycetocytes and sheath cells...

Sakurai, Makiko; Koga, Ryuichi; Tsuchida, Tsutomu; Meng, Xian-ying; Fukatsu, Takema

2005-01-01

250

Sca1, a previously undescribed paralog from autotransporter protein-encoding genes in Rickettsia species  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Abstract Background Among the 17 genes encoding autotransporter proteins of the "surface cell antigen" (sca) family in the currently sequenced Rickettsia genomes, ompA, sca5 (ompB) and sca4 (gene D), have been extensively used for identification and phylogenetic purposes for Rickettsia species. However, none of these genes is present in all 20 currently validated Rickettsia species. Of the remaining 14

Ngwamidiba Maxime; Blanc Guillaume; Raoult Didier; Fournier Pierre-Edouard

2006-01-01

251

Morphometric and quantitative characterization of atrial ganglion neurons from the intercaval region in dogs with dilated cardiomyopathy / Caracterização morfométrica e quantitativa dos neurônios ganglionares atriais da faixa intercaval de cães com cardiomiopatia dilatada  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Avaliaram-se quantitativa, morfométrica e qualitativamente os neurônios atriais da faixa intercaval de cães com cardiomiopatia dilatada (CMD). Os neurônios dos gânglios nervosos de cães com CMD eram maiores que os dos cães controle. A histopatologia do miocárdio ventricular e dos neurônios gangliona [...] res confirmou a CMD e demonstrou evidente processo degenerativo neuronal ganglionar. Cães com CMD em fase crônica apresentavam cardioneuropatia secundária, provavelmente pela privação da inervação parassimpática cardíaca. Abstract in english The quantity, morphometry, and quality of atrial neurons from the intercaval region in dogs with dilated cardiomyopathy (DCM) were evaluated. Dogs with DCM had greater ganglion neurons than control dogs. The histologic evaluation of the ventricular myocardium and ganglion neurons confirmed DCM and s [...] howed the degeneration of ganglion neurons. Dogs with chronic DCM had a secondary cardioneuropathy owing to impaired parasympathetic neural control.

A.A., Camacho; R., Oliveira-Alves; R.P., Klein; M.G., Sousa.

252

Detection of Rickettsia rickettsii and Rickettsia sp. in blood clots in 24 patients from different municipalities of the State of Sao Paulo, Brazil.  

Science.gov (United States)

The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit software. Seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced. PMID:17114719

Gehrke, Flávia Sousa; Mendes do Nascimento, Elvira Maria; Rodrigues de Souza, Eliana; Colombo, Silvia; Jacintho da Silva, Luiz; Schumaker, Teresinha Tizu Sato

2006-10-01

253

[Electrophoretic and immunochemical characteristics of proteins of Rickettsia prowazekii strains of various virulence].  

Science.gov (United States)

PAAG-electrophoresis of the isogenic pair of Rickettsia prowazekii strains E and Evir lysates demonstrate the similarity in polypeptide tracks. The different electrophoretic mobility of the Mr 30 Kd protein from these strains as compared with the mobility of analogous protein from the standard virulent Breinl strain is registered. In immunoblot experiments the specific rabbit antiserums obtained on the 30th day of infection with the Breinl, E or Evir strains demonstrate the presence of the different main antigens 60 Kd or 70 Kd. The difference evidently reflects the specificity of development of two forms of infection by the strains having different virulence. The surface tris-soluble antigens of Rickettsia prowazekii have the similar polypeptide contents and immunochemical properties. The main component of tris-soluble antigens Mr 130 Kd protein is not strain specific having the common thermolabile epitope. PMID:2501676

Eremeeva, M E; Lapina, E B; Balaeva, N M; Ignatovich, V F; Belousova, L S

1989-05-01

254

Outbreak of Rickettsia africae infections in participants of an adventure race in South Africa.  

Science.gov (United States)

African tick-bite fever, caused by Rickettsia africae and transmitted by Amblyomma ticks, is an emerging rickettsiosis in southern Africa. Because of increased tourism to this area, several cases in tourists have been reported recently. We report 13 cases of R. africae infection diagnosed in France that occurred in competitors returning from an adventure race in South Africa and compare our data with previously reported findings. Most of our patients presented with fever, headache, multiple inoculation eschars, and regional lymphadenopathies, but only 15.4% had a cutaneous rash. Diagnosis was confirmed either by isolation of R. africae from an eschar biopsy specimen or by serological methods, including cross-adsorption between R. africae and Rickettsia conorii. The purpose of this study was to raise physicians' awareness of R. africae infections in an attempt to facilitate the rapid diagnosis and treatment of imported African tick-bite fever in developed countries. PMID:9709882

Fournier, P E; Roux, V; Caumes, E; Donzel, M; Raoult, D

1998-08-01

255

Coinfection with Rickettsia helvetica and Herpes Simplex Virus 2 in a Young Woman with Meningoencephalitis.  

Science.gov (United States)

Herpes virus type 2 DNA was detected by PCR in the cerebrospinal fluid in a young woman presenting with headache, stiff neck and pleocytosis, and serological findings consistent with reactivation. Since she was exposed to ticks, Lyme disease and tick-borne encephalitis were excluded. Further investigation in an ongoing project, using PCR and sequencing of the amplified products, showed the presence of Rickettsia helvetica in the cerebrospinal fluid. The bacteria were also isolated in Vero cell culture, and microimmunofluorescence confirmed the development of antibodies against Rickettsia spp. with predominance of IgM reactivity consistent with recent infection. She was treated with antibiotics and improved rapidly. The patient could easily have been judged to have isolated herpes meningitis. Because Sweden and other European countries are endemic areas for rickettsioses, the paper reaffirms the importance of investigating for the presence of rickettsial infections in endemic areas in cases of meningitis of uncertain aetiology. PMID:22567472

Nilsson, Kenneth; Wallménius, Katarina; Påhlson, Carl

2011-01-01

256

Rickettsia conorii Malish and Israeli spotted fever strains causing disease in Portuguese Dogs  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Mediterranean spotted fever has the highest incidence in Portugak compared with opther endemic countries. However, few studies have been performed to understand the disease in the dogs, one of the most important host of Rhipicephalus sanguineus ticks, vector of Rickettsia conorii strains. Our main objective was to identify R. conorii as a cause of disease in dogs with clinical suspiction of tick-borne disease.

Alexandre, Nuno; Sousa, Rita; Santos, Ana Sofia; Nuncio, Maria Sofia; Boinas, Fernando; Bacellar, Fa?tima

2008-01-01

257

Tick cell culture isolation and growth of Rickettsia raoultii from Dutch Dermacentor reticulatus ticks  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. In an attempt to derive continuous Dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks originally collected as unfed adults in The Netherlands and maintained for up to 16 months. After several months, it became evident that cells in the primary cultures were infected with a Rickettsia-like intracellular o...

Alberdi, M. Pilar; Nijhof, Ard M.; Jongejan, Frans; Bell-sakyi, Lesley

2012-01-01

258

Coinfection with Rickettsia helvetica and Herpes Simplex Virus 2 in a Young Woman with Meningoencephalitis  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Herpes virus type 2 DNA was detected by PCR in the cerebrospinal fluid in a young woman presenting with headache, stiff neck and pleocytosis, and serological findings consistent with reactivation. Since she was exposed to ticks, Lyme disease and tick-borne encephalitis were excluded. Further investigation in an ongoing project, using PCR and sequencing of the amplified products, showed the presence of Rickettsia helvetica in the cerebrospinal fluid. The bacteria were also isolated in Vero cel...

2011-01-01

259

The Rickettsia conorii autotransporter protein Sca1 promotes adherence to nonphagocytic mammalian cells.  

Science.gov (United States)

The pathogenesis of spotted fever group (SFG) Rickettsia species, including R. conorii and R. rickettsii, is acutely dependent on adherence to and invasion of host cells, including cells of the mammalian endothelial system. Bioinformatic analyses of several rickettsia genomes revealed the presence of a cohort of genes designated sca genes that are predicted to encode proteins with homology to autotransporter proteins of Gram-negative bacteria. Previous work demonstrated that three members of this family, rOmpA (Sca0), Sca2, and rOmpB (Sca5) are involved in the interaction with mammalian cells; however, very little was known about the function of other conserved rickettsial Sca proteins. Here we demonstrate that sca1, a gene present in nearly all SFG rickettsia genomes, is actively transcribed and expressed in R. conorii cells. Alignment of Sca1 sequences from geographically diverse SFG Rickettsia species showed that there are high degrees of sequence identity and conservation of these sequences, suggesting that Sca1 may have a conserved function. Using a heterologous expression system, we demonstrated that production of R. conorii Sca1 in the Escherichia coli outer membrane is sufficient to mediate attachment to but not invasion of a panel of cultured mammalian epithelial and endothelial cells. Furthermore, preincubation of a recombinant Sca1 peptide with host cells blocked R. conorii cell association. Together, these results demonstrate that attachment to mammalian cells can be uncoupled from the entry process and that Sca1 is involved in the adherence of R. conorii to host cells. PMID:20176791

Riley, Sean P; Goh, Kenneth C; Hermanas, Timothy M; Cardwell, Marissa M; Chan, Yvonne G Y; Martinez, Juan J

2010-05-01

260

Rickettsia-like organism infecting juvenile sea-bass Dicentrarchus labrax  

Digital Repository Infrastructure Vision for European Research (DRIVER)

High levels of mortalities (20%) associated with abnormal swimming have been observed in farmed juveniles of seabass, Dicentrarchus labrax, in Mediterranean coast, southern France. Histopathology was obvious in the brain with nervOUS tissue necrosis and the presence of ceUs containing basophilic granules corresponding to smaU coccoid organisms. Ultrastructural examination revealed these organisms were rickettsia-like organisms morphologicaUy close to Piscirickettsia salmonis

Comps, Michel; Raymond, J. C.; Plassiart, G. N.

1996-01-01

 
 
 
 
261

Human and tick spotted fever group Rickettsia isolates from Israel: a genotypic analysis.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The genomes of spotted fever group rickettsiae isolated in different geographical areas of Israel (two from ticks and four from humans, obtained over a span of 20 years) were studied by polymerase chain reaction (PCR) and restriction endonuclease fragment length polymorphism (RFLP) analysis. The human isolates were obtained from patients suffering from rickettsial disease of different degrees of severity. The PCR products obtained with five pairs of oligonucleotide primers (two primer sets de...

Manor, E.; Ighbarieh, J.; Sarov, B.; Kassis, I.; Regnery, R.

1992-01-01

262

Molecular cloning and expression of Rickettsia tsutsugamushi genes for two major protein antigens in Escherichia coli.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Several polypeptide antigens of Rickettsia tsutsugamushi are recognized by human or primate convalescent sera and may be important protective immunogens. Molecular cloning and expression of the genes encoding the 110K (110 kilodalton) and 56K polypeptide antigens of R. tsutsugamushi Karp were accomplished in the lambda gt11 expression vector system. Southern blot analysis with the cloned fragments for the 56K polypeptide antigen (0.7 kilobases) and the 110K polypeptide antigen (5.4 kilobases)...

Oaks, E. V.; Stover, C. K.; Rice, R. M.

1987-01-01

263

Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

DNA sequences from specific genes, amplified by the polymerase chain reaction technique, were used as substrata for nonisotopic restriction endonuclease fragment length polymorphism differentiation of rickettsial species and genotypes. The products amplified using a single pair of oligonucleotide primers (derived from a rickettsial citrate synthase gene sequence) and cleaved with restriction endonucleases were used to differentiate almost all recognized species of rickettsiae. A second set of...

Regnery, R. L.; Spruill, C. L.; Plikaytis, B. D.

1991-01-01

264

Taxonomic Relationships among Spotted Fever Group Rickettsiae as Revealed by Antigenic Analysis with Monoclonal Antibodies  

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The spotted fever group (SFG) is made up of more than 20 different rickettsial species and strains. Study of the taxonomic relationships among the group has been attempted by phenotypic, genotypic, and phylogenetic analyses. In this study, we determined taxonomic relationships among the SFG rickettsiae by comparative analysis of immunogenic epitopes reactive against a panel of monoclonal antibodies. A total of 98 monoclonal antibodies, which were directed against epitopes on the major immunod...

Xu, Wenbin; Raoult, Didier

1998-01-01

265

Ehrlichia phagocytophila genogroup rickettsiae in ixodid ticks from California collected in 1995 and 1996.  

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A total of 1,246 ixodid ticks collected in 1995 and 1996 from seven California counties were examined for the presence of Ehrlichia phagocytophila genogroup rickettsiae by using a nested PCR technique. Of 1,112 adult Ixodes pacificus Cooley and Kohls ticks tested, nine pools, each containing five ticks, were positive (minimum percentage of ticks harboring detectable ehrlichiae, 0.8%). Positive ticks were limited to four of the seven counties (Sonoma, El Dorado, Santa Cruz, and Orange). In San...

Barlough, J. E.; Madigan, J. E.; Kramer, V. L.; Clover, J. R.; Hui, L. T.; Webb, J. P.; Vredevoe, L. K.

1997-01-01

266

Rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro.  

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The endothelial cell is the putative primary target cell in humans infected with Rickettsia rickettsii, the etiological agent of Rocky Mountain spotted fever. Although the clinical manifestations of infection by this organism are well documented, the mechanism of injury to the endothelial cell is not understood. The ability to culture human endothelial cells in vitro provides a unique system with which to study this host-parasite interaction directly. Human vascular endothelial cells derived ...

Silverman, D. J.

1984-01-01

267

Tick cell culture isolation and growth of Rickettsia raoultii from Dutch Dermacentor reticulatus ticks  

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Tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. In an attempt to derive continuous Dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks originally collected as unfed adults in The Netherlands and maintained for up to 16 months. After several months, it became evident that cells in the primary cultures were infected with a Rickettsia-like intracellular organism. Sup...

Alberdi, M. Pilar; Nijhof, Ard M.; Jongejan, Frans; Bell-sakyi, Lesley

2012-01-01

268

Biochemical and immunochemical analysis of Rickettsia rickettsii strains of various degrees of virulence.  

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Six strains of Rickettsia rickettsii from Montana and North Carolina were examined in an effort to identify rickettsial constituents associated with virulence. Fever responses, scrotal reactions, and mortalities of male guinea pigs inoculated intraperitoneally with 1,000 PFU of rickettsial strains revealed that the two Montana patient strains ( Sheila Smith and Norgaard ) and one Montana strain ( Sawtooth female 2) from the wood tick, Dermacentor andersoni, could be placed in the group of hig...

Anacker, R. L.; Philip, R. N.; Williams, J. C.; List, R. H.; Mann, R. E.

1984-01-01

269

Coinfection with Rickettsia helvetica and Herpes simplex virus 2 in a Young Woman with Meningoencephalitis  

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Herpes virus type 2 DNA was detected by PCR in the cerebrospinal fluid in a young woman presenting with headache, stiff neck and pleocytosis and serological findings consistent with reactivation. Since she was exposed to ticks, Lyme disease and tick-borne-encephalitis were excluded. Further investigation in an ongoing project, using PCR and sequencing of the amplified products, showed the presence of Rickettsia helvetica in the cerebrospinal fluid. The bacteria were also isolated in Vero cell...

Nilsson, Kenneth; Wallme?nius, Katarina; Pa?hlson, Carl

2011-01-01

270

Spotted fever group Rickettsia infecting ticks (Acari: Ixodidae) in the state of Santa Catarina, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english During 2006-2008, a total of 260 adult ticks were collected from domestic and wild animals in different regions of the state of Santa Catarina (SC), Brazil, including areas where human cases of Brazilian spotted fever have been reported. Collected ticks belonging to nine species (Amblyomma aureolatu [...] m, Amblyomma cajennense, Amblyomma dubitatum, Amblyomma longirostre, Amblyomma ovale, Amblyomma tigrinum, Dermacentor nitens, Rhipicephalus microplus and Rhipicephalus sanguineus) were tested by polymerase chain reaction (PCR) for rickettsial infection. Overall, eight (3.1%) ticks were found to be infected with Rickettsia species. After sequencing the PCR products, we determined that the sequences generated from three A. aureolatum, one A. ovale and one R. sanguineus from the municipality of Blumenau, one A. ovale from the municipality of Águas Mornas and one A. ovale from the municipality of Urussanga were identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia parkeri strain Atlantic rainforest. The sequence generated from one A. longirostre from Blumenau was 100% identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia amblyommii strain AL. Because R. parkeri strain Atlantic rainforest was recently shown to have caused two cases of human spotted fever in other states of Brazil, the role of this rickettsial agent as a possible etiological agent of spotted fever in SC is discussed.

Medeiros, Alessandra Pereira; Souza, Antonio Pereira de; Moura, Anderson Barbosa de; Lavina, Marcia Sangaletti; Bellato, Valdomiro; Sartor, Amélia Aparecida; Nieri-Bastos, Fernanda Aparecida; Richtzenhain, Leonardo José; Labruna, Marcelo Bahia.

271

Rickettsia actin-based motility occurs in distinct phases mediated by different actin nucleators.  

Science.gov (United States)

Many intracellular bacterial pathogens undergo actin-based motility to promote cell-cell spread during infection [1]. For each pathogen, motility was assumed to be driven by a single actin polymerization pathway. Curiously, spotted fever group Rickettsia differ from other pathogens in possessing two actin-polymerizing proteins. RickA, an activator of the host Arp2/3 complex, was initially proposed to drive motility [2, 3]. Sca2, a mimic of host formins [4, 5], was later shown to be required for motility [6]. Whether and how their activities are coordinated has remained unclear. Here, we show that each protein directs an independent mode of Rickettsia parkeri motility at different times during infection. Early after invasion, motility is slow and meandering, generating short, curved actin tails that are enriched with Arp2/3 complex and cofilin. Early motility requires RickA and Arp2/3 complex and is correlated with transient RickA localization to the bacterial pole. Later in infection, motility is faster and directionally persistent, resulting in long, straight actin tails. Late motility is independent of Arp2/3 complex and RickA and requires Sca2, which accumulates at the bacterial pole. Both motility pathways facilitate cell-to-cell spread. The ability to exploit two actin assembly pathways may allow Rickettsia to establish an intracellular niche and spread between diverse cells throughout a prolonged infection. PMID:24361066

Reed, Shawna C O; Lamason, Rebecca L; Risca, Viviana I; Abernathy, Emma; Welch, Matthew D

2014-01-01

272

Spotted fever group Rickettsia infecting ticks (Acari: Ixodidae in the state of Santa Catarina, Brazil  

Directory of Open Access Journals (Sweden)

Full Text Available During 2006-2008, a total of 260 adult ticks were collected from domestic and wild animals in different regions of the state of Santa Catarina (SC, Brazil, including areas where human cases of Brazilian spotted fever have been reported. Collected ticks belonging to nine species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Amblyomma longirostre, Amblyomma ovale, Amblyomma tigrinum, Dermacentor nitens, Rhipicephalus microplus and Rhipicephalus sanguineus were tested by polymerase chain reaction (PCR for rickettsial infection. Overall, eight (3.1% ticks were found to be infected with Rickettsia species. After sequencing the PCR products, we determined that the sequences generated from three A. aureolatum, one A. ovale and one R. sanguineus from the municipality of Blumenau, one A. ovale from the municipality of Águas Mornas and one A. ovale from the municipality of Urussanga were identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia parkeri strain Atlantic rainforest. The sequence generated from one A. longirostre from Blumenau was 100% identical to the corresponding partial rickettsial ompA gene sequence of Rickettsia amblyommii strain AL. Because R. parkeri strain Atlantic rainforest was recently shown to have caused two cases of human spotted fever in other states of Brazil, the role of this rickettsial agent as a possible etiological agent of spotted fever in SC is discussed.

Alessandra Pereira Medeiros

2011-12-01

273

Rhipicephalus ticks infected with Rickettsia and Coxiella in Southern Switzerland (Canton Ticino).  

Science.gov (United States)

Ticks of the Rhipicephalus sanguineus species complex may be vector of various pathogens including Rickettsia conorii (the etiological agent of the Mediterranean spotted fever) and Coxiella burnetii (cause of the Query (Q) fever). R. sanguineus ticks have been imported in several parts of central and northern Europe, especially in environments such as kennels and houses providing the appropriate microclimatic conditions and the blood source necessary for their survival. Since 1940 these ticks have occasionally been recorded in Switzerland. In Ticino (the southern part of Switzerland), they have been reported since 1980 and their probable establishment in this area has been suggested in the '90s. By means of PCR and direct sequencing, we tested the identity of these ticks (using 12S rDNA gene) and the occurrence of Rickettsia spp. (using 16S rDNA, gltA and OmpA genes) as well as Coxiella sp. (using 16S rDNA). The results indicated that in Ticino, two different tick species coexist, i.e. R. sanguineus sensu stricto and Rhipicephalus turanicus. A few individuals of R. sanguineus sensu stricto are infected with Rickettsia massiliae/Bar29, which are strains of unknown pathogenicity. Coxiella sp., an endosymbiont of Rhipicephalus ticks, has also been identified in both tick species. Due to climatic changes towards global warming, imported tick species may therefore adapt to new area and might be considered as epidemiological markers for a number of infectious agents transmitted by them. PMID:12797987

Bernasconi, Marco V; Casati, Simona; Péter, Olivier; Piffaretti, Jean-Claude

2002-12-01

274

Detection of Leishmania infantum, Babesia canis, and rickettsiae in ticks removed from dogs living in Italy.  

Science.gov (United States)

The aims of this study were to determine natural infections by Anaplasma phagocytophilum/Anaplasma platys, Bartonella henselae, Ehrlichia canis, Leishmania infantum, Rickettsia spp., Babesia spp., and Hepatozoon spp. by molecular methods in ticks (n=91) removed from dogs with clinical signs and laboratory abnormalities compatible with tick-borne diseases (n=22) living in Italy and to assess the distribution and species of ticks encountered. Ticks from dogs living in southern Italy were all identified as Rhipicephalus sanguineus (n=25), ticks from central Italy included Rh. sanguineus (n=8) and Ixodes ricinus (n=9), ticks from northern Italy included Rh. sanguineus (n=45), Dermacentor marginatus (n=3), and one I. ricinus. Leishmania infantum, Rickettsia spp., and Babesia canis were the only pathogens detected in 7 (8%), 4 (4%), and 2 (2%) out of 91 ticks, respectively. L. infantum was detected in I. ricinus from central Italy and in Rh. sanguineus from northern and central Italy. Rickettsia conorii and Ri. massiliae were detected in Rh. sanguineus ticks from central and southern Italy (Sicily), respectively. Bab. canis was detected in D. marginatus ticks from northern Italy. PMID:23182545

Trotta, Michele; Nicetto, Martina; Fogliazza, Alessandro; Montarsi, Fabrizio; Caldin, Marco; Furlanello, Tommaso; Solano-Gallego, Laia

2012-12-01

275

Detection of Rickettsia felis and Rickettsia typhi and seasonal prevalence of fleas collected from small mammals at Gyeonggi Province in the Republic of Korea.  

Science.gov (United States)

Fleas were collected from live-captured small mammals to identify flea-borne pathogens, host associations, and seasonal prevalence of flea species, as part of the 65th Medical Brigade rodent-borne disease surveillance program at 20 military installations and training sites, Gyeonggi Province, Republic of Korea, 2005-2007. A total of 1251 fleas were recovered from 2833 small mammals. Apodemus agrarius, the striped field mouse, accounted for 93.1% (2,637/2,833) of all small mammals captured, followed by Crocidura lasiura (3.1%), Mus musculus (1.3%), Microtus fortis (0.7%), Myodes regulus (0.7%), Micromys minutus (0.5%), Rattus norvegicus (0.4%), Tscherskia triton (0.1%), Apodemus peninsulae (regulus) to a low of 5.3% (M. fortis). Flea indices among infested mammals were highest for R. norvegicus (2.50), followed by C. lasiura (2.20), A. agrarius (1.71), M. regulus (1.20), M. musculus (1.0), and M. fortis (1.0). The predominant flea species collected were Stenoponia sidimi (56.5%), followed by Ctenophthalmus congeneroides (38.3%) and Rhadinopsylla insolita (3.9%). The minimum field infection rates [number of positive pools/total number of fleas (600)] for Rickettsia typhi and for Rickettsia felis were 1.7% and 1.0%, respectively. PMID:21612536

Ko, Sungjin; Kim, Heung-Chul; Yang, Young-Cheol; Chong, Sung-Tae; Richards, Allen L; Sames, William J; Klein, Terry A; Kang, Jun-Gu; Chae, Joon-Seok

2011-09-01

276

Prevalence of Rickettsiales (Anaplasma phagocytophilum and Rickettsia spp.) in hard ticks (Ixodes ricinus) in the city of Hamburg, Germany.  

Science.gov (United States)

To narrow the gap of missing knowledge on Rickettsia spp. and Anaplasma phagocytophilum infections in ticks in northwestern Germany and, at the same time, to provide first prevalence data on these pathogens in the city of Hamburg, a total of 1,400 questing Ixodes ricinus ticks were collected at ten different public green areas from April until October 2011. Ticks were examined using probe-based quantitative real-time PCR. A percentage of 3.6% (51/1,400) ticks were tested positive for A. phagocytophilum infections divided into 2.1% (3/141) adults [1.7% (1/60) females and 2.5% (2/81) males] and 3.8% (48/1,259) nymphs. The percentage of infected ticks per sampling site varied statistically significantly from 0.7% (1/140) to 12.1% (17/140), whereas between sampling months, no statistically significant differences were observed (2.0-6.5%, 4-13/140). The overall Rickettsia spp. infection rate was 52.5% (735/1,400). In adult ticks, Rickettsia spp. infection rate was 56% (79/141) divided into 61.7% (37/60) infected females and 51.9% (42/81) infected males. Nymphs showed an infection rate of 52.1% (656/1,259). In contrast to A. phagocytophilum infections, no statistically significant differences in Rickettsia spp. infection rates among sampling sites (44.3-63.6%, 62-89/140) were observed, whereas seasonal variations were obvious: the percentage of Rickettsia-positive ticks was significantly lower in April (36.5%, 73/200) and May (29.5%, 59/200) compared to the summer and fall months (55.0-64.5%, 110-129/200). Rickettsia species differentiation via real-time pyrosequencing revealed Rickettsia helvetica as the only occurring species. Co-infections with both Rickettsia spp. and A. phagocytophilum were detected in 2.0% (28/1,400) of the ticks. The present study revealed that in the city of Hamburg, the tick infection rate with A. phagocytophilum is comparable with other German data, whereas the Rickettsia spp. infection rate of 52.5% is by far the highest prevalence detected in Germany so far. As the city of Hamburg has 1.8 million inhabitants and attracts millions of tourists every year, the potential health risk should not be underestimated. PMID:24728556

May, Kathrin; Strube, Christina

2014-06-01

277

Borrelia, Coxiella, and Rickettsia in Carios capensis (Acari: Argasidae) from a brown pelican (Pelecanus occidentalis) rookery in South Carolina, USA.  

Science.gov (United States)

Argasid ticks are vectors of viral and bacterial agents of humans and animals. Carios capensis, a tick of seabirds, infests the nests of brown pelicans, Pelecanus occidentalis, and other ground nesting birds along the coast of South Carolina. This tick is associated with pelican nest abandonment and could pose a threat to humans visiting pelican rookeries if visitors are exposed to ticks harboring infectious agents. We collected ticks from a pelican rookery on Deveaux Bank, South Carolina and screened 64 individual ticks, six pools of larvae, and an egg mass for DNA from Bartonella, Borrelia, Coxiella, and Rickettsia by polymerase chain reaction amplification and sequencing. Ticks harbored DNA from "Borrelia lonestari", a novel Coxiella sp., and three species of Rickettsia, including Rickettsia felis and two undescribed Rickettsia spp. DNA from the Coxiella and two undescribed Rickettsia were detected in unfed larvae that emerged in the laboratory, which implies these agents are transmitted vertically by female ticks. We partially characterize the novel Coxiella by molecular means. PMID:16821092

Reeves, Will K; Loftis, Amanda D; Sanders, Felicia; Spinks, Mark D; Wills, William; Denison, Amy M; Dasch, Gregory A

2006-01-01

278

Serosurvey for tick-borne diseases in dogs from the Eastern Amazon, Brazil Pesquisa Sorológica por doenças transmitidas por carrapatos em cães da Amazônia oriental, Brasil  

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Full Text Available Canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in Brazilian dogs. Few studies have focused attention in surveying tick-borne diseases in the Brazilian Amazon region. A total of 129 blood samples were collected from dogs living in the Brazilian eastern Amazon. Seventy-two samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from Santarém municipality were collected. Serum samples were submitted to Indirect Immunofluorescence Assay (IFA with antigens of Babesia canis vogeli, Ehrlichia canis, and six Rickettsia species. The frequency of dogs containing anti-B. canis vogeli, anti-E. canis, and anti-Rickettsia spp. antibodies was 42.6%, 16.2%, and 31.7%, respectively. Anti-B. canis vogeli antibodies were detected in 59.6% of the urban dogs, and in 29.1% of the rural dogs (P Ehrliquiose canina e babesiose canina são as doenças parasitárias transmitidas por carrapatos de maior prevalência em cães do Brasil. Poucos estudos pesquisaram doenças transmitidas por carrapatos na região da Amazônia brasileira. Um total de 129 amostras de sangue foram colhidas de cães da Amazônia oriental brasileira. Setenta e dois cães eram de áreas rurais de 19 municípios do Estado do Pará, e 57 amostras foram colhidas de cães errantes vadios da área urbana do município de Santarém-PA. As amostras de soro foram submetidas ao ensaio de imunofluorescência indireta, com antígenos de Babesia canis vogeli, Ehrlichia canis, e seis espécies de Rickettsia. A frequência de cães com anticorpos anti-B. canis vogeli, anti-E. canis, e anti-Rickettsia spp. foi de 42,6%, 16,2% e 31,7%, respectivamente. Anticorpos anti-B. canis vogeli foram detectados em 59,6% dos cães urbanos, e em 29,1% dos cães rurais (P < 0.05. Para E. canis, a soroprevalência foi parecida entre os cães urbanos (15,7% e rurais (16,6%. Para Rickettsia spp., cães rurais apresentaram prevalência (P < 0.05 significativamente maior (40,3% do que os cães urbanos (21,1%. Esse primeiro estudo sobre agentes transmitidos por carrapatos entre cães da Amazônia oriental brasileira indica que estes animais estão expostos a vários agentes. Estes incluem Babesia principalmente na área urbana, Riquétsias do grupo da Febre Maculosa principalmente nas áreas rurais, e Erliquia em cães de ambas as áreas, rural e urbana.

Mariana Granziera Spolidorio

279

Importation of exotic ticks and tick-borne spotted fever group rickettsiae into the United States by migrating songbirds.  

Science.gov (United States)

Birds are capable of carrying ticks and, consequently, tick-transmitted microorganisms over long distances and across geographical barriers such as oceans and deserts. Ticks are hosts for several species of spotted fever group rickettsiae (SFGR), which can be transmitted to vertebrates during blood meals. In this study, the prevalence of this group of rickettsiae was examined in ticks infesting migratory songbirds by using polymerase chain reaction (PCR). During the 2009 and 2010 spring migration season, 2064 northward-migrating passerine songbirds were examined for ticks at Johnson Bayou, Louisiana. A total of 91 ticks was removed from 35 individual songbirds for tick species identification and spotted fever group rickettsia detection. Ticks were identified as Haemaphysalis juxtakochi (n=38, 42%), Amblyomma longirostre (n=22, 24%), Amblyomma nodosum (n=17, 19%), Amblyomma calcaratum (n=11, 12%), Amblyomma maculatum (n=2, 2%), and Haemaphysalis leporispalustris (n=1, 1%) by comparing their 12S rDNA gene sequence to homologous sequences in GenBank. Most of the identified ticks were exotic species originating outside of the United States. The phylogenetic analysis of the 71 ompA gene sequences of the rickettsial strains detected in the ticks revealed the occurrence of 6 distinct rickettsial genotypes. Two genotypes (corresponding to a total of 28 samples) were included in the Candidatus Rickettsia amblyommii clade (less than 1% divergence), 2 of them (corresponding to a total of 14 samples) clustered with Rickettsia sp. "Argentina" with less than 0.2% sequence divergence, and 2 of them (corresponding to a total of 27 samples), although closely related to the R. parkeri-R. africae lineage (2.50-3.41% divergence), exhibited sufficient genetic divergence from its members to possibly constitute a new rickettsial genotype. Overall, there does not seem to be a specific relationship between exotic tick species, the rickettsiae they harbor, or the reservoir competence of the corresponding bird species. PMID:24252263

Mukherjee, Nabanita; Beati, Lorenza; Sellers, Michael; Burton, Laquita; Adamson, Steven; Robbins, Richard G; Moore, Frank; Karim, Shahid

2014-03-01

280

Análisis de las recidivas locorregionales por cáncer de mama / Analysis of locoregional relapses from breast cancer  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Introducción: la recidiva locorregional, luego de una intervención quirúrgica por cáncer de mama, es una gran preocupación para el paciente y una frustración para el cirujano actuante. Objetivos: realizar un estudio descriptivo de las variables asociadas a la recurrencia tumoral, en 949 pacientes op [...] eradas. Métodos: se confeccionó una base de datos con las pacientes operadas desde 2005 hasta 2010, se identificaron las variables y análisis univariables y multivariables para determinar si existió asociación estadística. Resultados: se apreció que la incidencia fue de 4,4 %. La intervención quirúrgica previa más frecuente fue la mastectomía radical modificada en 52,4 %, y 26,2 % de los casos que tenían metástasis ganglionares axilares en ese momento. El tipo histológico más frecuente fue el carcinoma ductal infiltrante, de alto grado de malignidad. La infiltración vascular y linfática estuvo presente en 42,8 %. El tratamiento definitivo más empleado fue la exéresis de la recidiva más radioterapia. El análisis multivariables solo asoció la metástasis ganglionar con la reaparición de la enfermedad. Conclusiones: la metástasis ganglionar axilar sigue siendo un factor de mal pronóstico en el seguimiento extenso de la paciente. Abstract in english Introduction: locoregional relapses after a breast cancer surgery is a big concern for the patient and a frustration to the performing surgeon. Objectives: to perform a descriptive study of the tumor recurrence-associated variables in 949 surgical patients. Methods: a database containing the data fr [...] om operated patients in the period of 2005 through 2010 was made; the variables were identified and the univariate and multivariate analyses allowed determining the probable statistical association. Results: at was observed that the incidence of locoregional recurrence was 4.4 %. The most common previous surgery was modified radical mastectomy in 52.4 % and 26.2 % of cases with axillary ganglionic metastasis at that time. The most frequent histological type was highly malignant infiltrating bile duct carcinoma. The vascular and lymphatic infiltration occurred in 42.8 % of patients. The most used treatment was removal of recurrence plus radiotherapy. The multivariate analysis was associated only to ganglionic metastasis with the reappearance of disease. Conclusions: axillary ganglionic metastasis is still a factor indicating bad prognosis in the long follow-up of a patient.

Pérez Gutiérrez, Oscar Alberto; Montejo Viamontes, Narciso; Jorge Lázaro, Loys; Castro Cruz, Rafael; Estrada González, Amauris.

 
 
 
 
281

The influence of temperature and pH on the growth of Rickettsia conorii in irradiated mammalian cells  

International Nuclear Information System (INIS)

The temperature range for optimum growth of Rickettsia conorii in suspension culture of gamma-irradiated L cells was 32 to 38 degC, resulting in rickettsial doubling times between 4.1 and 6.0 hrs. An asynchronous release of Rickettsia conorii from host cells was suggested by the constant increase in percent cells infected over a 36 hrs period. Rickettsial growth was optimal at neutral to slightly alkaline extracellular pH levels. A moderately acidic pH, however, resulted in an increase in doubling time from 4.1 to 7.8 hrs. (author)

1979-01-01

282

Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique  

Directory of Open Access Journals (Sweden)

Full Text Available Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii and is transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with 24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is Amblyomma parvum.

K. Dzul-Rosado

2013-09-01

283

Inhibition of Rickettsia conorii growth by recombinant tumor necrosis factor alpha: enhancement of inhibition by gamma interferon.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Purified human recombinant tumor necrosis factor alpha (rTNF-alpha) inhibited the growth of Rickettsia conorii (Casablanca strain) in HEp-2 cell culture. The effect was observed when the cells were pretreated with rTNF-alpha or when rTNF-alpha was added after adsorption of the rickettsiae. The inhibitory effect of rTNF-alpha on rickettsial growth was enhanced by gamma interferon. Cycloheximide had no effect on inhibition of the rickettsial yield, suggesting that de novo protein synthesis is n...

Manor, E.; Sarov, I.

1990-01-01

284

Experimental infection of dogs with a Brazilian strain of Rickettsia rickettsii: clinical and laboratory findings  

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Full Text Available The bacterium Rickettsia rickettsii is the etiological agent of an acute, severe disease called Rocky Mountain spotted fever in the United States or Brazilian spotted fever (BSF in Brazil. In addition to these two countries, the disease has also been reported to affect humans in Mexico, Costa Rica, Panama, Colombia and Argentina. Like humans, dogs are also susceptible to R. rickettsii infection. However, despite the wide distribution of R. rickettsii in the Western Hemisphere, reports of R. rickettsii-induced illness in dogs has been restricted to the United States. The present study evaluated the pathogenicity for dogs of a South American strain of R. rickettsii. Three groups of dogs were evaluated: group 1 (G1 was inoculated ip with R. rickettsii; group 2 (G2 was infested by R. rickettsii-infected ticks; and the control group (G3 was infested by uninfected ticks. During the study, no clinical abnormalities, Rickettsia DNA or R. rickettsii-reactive antibodies were detected in G3. In contrast, all G1 and G2 dogs developed signs of rickettsial infection, i.e., fever, lethargy, anorexia, ocular lesions, thrombocytopenia, anemia and detectable levels of Rickettsia DNA and R. rickettsii-reactive antibodies in their blood. Rickettsemia started 3-8 days after inoculation or tick infestation and lasted for 3-13 days. Our results indicate that a Brazilian strain of R. rickettsii is pathogenic for dogs, suggesting that canine clinical illness due to R. rickettsii has been unreported in Brazil and possibly in the other South American countries where BSF has been reported among humans.

Eliane M Piranda

2008-11-01

285

Experimental infection of dogs with a Brazilian strain of Rickettsia rickettsii: clinical and laboratory findings  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english The bacterium Rickettsia rickettsii is the etiological agent of an acute, severe disease called Rocky Mountain spotted fever in the United States or Brazilian spotted fever (BSF) in Brazil. In addition to these two countries, the disease has also been reported to affect humans in Mexico, Costa Rica, [...] Panama, Colombia and Argentina. Like humans, dogs are also susceptible to R. rickettsii infection. However, despite the wide distribution of R. rickettsii in the Western Hemisphere, reports of R. rickettsii-induced illness in dogs has been restricted to the United States. The present study evaluated the pathogenicity for dogs of a South American strain of R. rickettsii. Three groups of dogs were evaluated: group 1 (G1) was inoculated ip with R. rickettsii; group 2 (G2) was infested by R. rickettsii-infected ticks; and the control group (G3) was infested by uninfected ticks. During the study, no clinical abnormalities, Rickettsia DNA or R. rickettsii-reactive antibodies were detected in G3. In contrast, all G1 and G2 dogs developed signs of rickettsial infection, i.e., fever, lethargy, anorexia, ocular lesions, thrombocytopenia, anemia and detectable levels of Rickettsia DNA and R. rickettsii-reactive antibodies in their blood. Rickettsemia started 3-8 days after inoculation or tick infestation and lasted for 3-13 days. Our results indicate that a Brazilian strain of R. rickettsii is pathogenic for dogs, suggesting that canine clinical illness due to R. rickettsii has been unreported in Brazil and possibly in the other South American countries where BSF has been reported among humans.

Piranda, Eliane M; Faccini, João Luis H; Pinter, Adriano; Saito, Tais B; Pacheco, Richard C; Hagiwara, Mitika K; Labruna, Marcelo B.

286

CULTIVATION OF RICKETTSIA-LIKE MICROORGANISMS FROM THE ROCKY MOUNTAIN SPOTTED FEVER TICK, DERMACENTOR ANDERSONI.  

Science.gov (United States)

A systematic study of 74 ticks, the infectivity or non-infectivity of which was determined by biting experiments, inoculation of emulsions, and specific immunity tests, showed the presence in some instances of several types of microorganisms morphologically resembling the inciting microorganism of spotted fever. The most frequently isolated was B. rickettsiformis, n. sp., those less commonly encountered were B. pseudoxerosis, n. sp., and B. equidistans, n. sp. These organisms are non-pathogenic for the guinea pig, rabbit, and Macacus rhesus. In morphological features they resemble the forms found in smears and sections of the ticks, yet their presence had no relation to infectivity. Immunologically they are not related to the spotted fever virus. All three are pleomorphic under cultural conditions, and the question arises whether or not the minute non-pathogenic Rickettsia forms and the somewhat coarser symbionts found in Dermacentor andersoni are morphological variations due to variations in nutrition, oxygen tension, tissue reactions, etc., in the different tissues and cells in which the organisms are embedded. At all events, the differentiation of the non-pathogenic Rickettsia-like organisms from Dermacentroxenus rickettsi is extremely difficult. In definitely infective ticks of the present study intranuclear forms were not constant. A point of special interest is that these non-pathogenic micro-organisms from ticks grow best at room temperature, in this respect resembling culturally certain flagellates inhabiting the alimentary tract of insects. The difficulty of obtaining initial growth on artificial media and the gradual adaptation to less specialized media are other notable characteristics of these organisms. The possibility that B. rickettsiformis is a non-pathogenic phase of the spotted fever organism, comparable with the avirulent flagellate culture forms of Leishmanias, seems remote in view of the negative immunological findings. Hereditary transmission of B. rickettsiformis is clearly indicated by its presence in large numbers in ovaries and egg cells, a characteristic also of the spotted fever organism and of other insect-borne Rickettsiae. PMID:19869142

Noguchi, H

1926-03-31

287

Rickettsia amblyommii infecting Amblyomma auricularium ticks in Pernambuco, northeastern Brazil: isolation, transovarial transmission, and transstadial perpetuation.  

Science.gov (United States)

This study investigated rickettsial infection in Amblyomma auricularium ticks from the state of Pernambuco, northeastern Brazil. An engorged female of A. auricularium collected from a skunk (Conepatus semistriatus) was sent alive to the laboratory, where the female was found through molecular analysis to be infected by Rickettsia amblyommii. This engorged female oviposited, and its offspring was reared through three consecutive generations, always using tick-naïve rabbits to feed the ticks. PCR performed on five egg pools, 10 larvae, 10 nymphs, and 10 adults of each of the three generations always yielded rickettsial DNA, indicating maintenance of rickettsial infection in the ticks by transstadial and transovarial passages. DNA sequences of random PCR products from eggs, larvae, nymphs, and adults were identified as R. amblyommii. All infested rabbits seroconverted to R. amblyommii antigens at the 21(st) day after infestation, indicating that larvae, nymphs, and adults transmitted R. amblyommii through parasitism. However, no infested rabbit presented fever or any clinical alteration during the experimental period. Rickettsiae were successfully isolated from the two A. auricularium females, and the isolates were established in Vero cell culture. Molecular characterization of the isolates confirmed R. amblyommii by sequencing partial gltA, ompA, and ompB genes. From another sample of 15 A. auricularium adult ticks collected from two armadillos (Euphractus sexcinctus), eight (53.3%) were infected by R. amblyommii. This study reports R. amblyommii infecting the tick A. auricularium for the first time. This is also the first report of rickettsia infecting ticks in the northeastern region of Brazil. PMID:23705586

Saraiva, Danilo G; Nieri-Bastos, Fernanda A; Horta, Maurício C; Soares, Herbert S; Nicola, Patricia A; Pereira, Luiz Cezar M; Labruna, Marcelo B

2013-09-01

288

Dissemination of Spotted Fever Rickettsia Agents in Europe by Migrating Birds  

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Migratory birds are known to play a role as long-distance vectors for many microorganisms. To investigate whether this is true of rickettsial agents as well, we characterized tick infestation and gathered ticks from 13,260 migratory passerine birds in Sweden. A total of 1127 Ixodes spp. ticks were removed from these birds and the extracted DNA from 957 of them was available for analyses. The DNA was assayed for detection of Rickettsia spp. using real-time PCR, followed by DNA sequencing for s...

Elfving, Karin; Olsen, Bjo?rn; Bergstro?m, Sven; Waldenstro?m, Jonas; Lundkvist, A?ke; Sjo?stedt, Anders; Mejlon, Hans; Nilsson, Kenneth

2010-01-01

289

Detection of Rickettsia japonica in Haemaphysalis longicornis ticks by restriction fragment length polymorphism of PCR product.  

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PCR was applied to the detection of Rickettsia japonica, the causative agent of Oriental spotted fever (OSF), in ticks collected at two sites of the Muroto area on Shikoku Island, a major area in Japan where OSF is endemic. Primer pair Rr190.70p and Rr190.602n of the R. rickettsii 190-kDa antigen gene sequence of Regnery and others (R.L. Regnery, C.L. Spruill, and B.D. Plikaytis, J. Bacteriol. 173:1576-1589, 1991) primed the DNA extracted from Haemaphysalis longicornis ticks but not those ext...

1995-01-01

290

Cloning and expression of the Rickettsia prowazekii ADP/ATP translocator in Escherichia coli.  

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Cosmid clone banks of Rickettsia prowazekii genomic DNA were established in Escherichia coli and screened for expression of the rickettsial carrier-mediated ADP/ATP translocator. Out of 2700 clones screened, a single clone, designated MOB286, accumulated radioactivity when incubated with [alpha-32P]ATP in 100 mM sodium phosphate buffer. This clone carried a plasmid, pMW286, containing a 9-kilobase-pair insert of rickettsial DNA, as established by DNA X DNA hybridizations. Transformation studi...

Krause, D. C.; Winkler, H. H.; Wood, D. O.

1985-01-01

291

Serosurvey for tick-borne diseases in dogs from the Eastern Amazon, Brazil / Pesquisa Sorologica por doencas transmitidas por carrapatos em caes da Amazonia oriental, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Ehrliquiose canina e babesiose canina são as doenças parasitárias transmitidas por carrapatos de maior prevalência em cães do Brasil. Poucos estudos pesquisaram doenças transmitidas por carrapatos na região da Amazônia brasileira. Um total de 129 amostras de s [...] angue foram colhidas de cães da Amazônia oriental brasileira. Setenta e dois cães eram de áreas rurais de 19 municípios do Estado do Pará, e 57 amostras foram colhidas de cães errantes vadios da área urbana do município de Santarém-PA. As amostras de soro foram submetidas ao ensaio de imunofluorescência indireta, com antígenos de Babesia canis vogeli, Ehrlichia canis, e seis espécies de Rickettsia. A frequência de cães com anticorpos anti-B. canis vogeli, anti-E. canis, e anti-Rickettsia spp. foi de 42,6%, 16,2% e 31,7%, respectivamente. Anticorpos anti-B. canis vogeli foram detectados em 59,6% dos cães urbanos, e em 29,1% dos cães rurais (P < 0.05). Para E. canis, a soroprevalência foi parecida entre os cães urbanos (15,7%) e rurais (16,6%). Para Rickettsia spp., cães rurais apresentaram prevalência (P < 0.05) significativamente maior (40,3%) do que os cães urbanos (21,1%). Esse primeiro estudo sobre agentes transmitidos por carrapatos entre cães da Amazônia oriental brasileira indica que estes animais estão expostos a vários agentes. Estes incluem Babesia principalmente na área urbana, Riquétsias do grupo da Febre Maculosa principalmente nas áreas rurais, e Erliquia em cães de ambas as áreas, rural e urbana. Abstract in english Canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in Brazilian dogs. Few studies have focused attention in surveying tick-borne diseases in the Brazilian Amazon region. A total of 129 blood samples were collected from dogs living in the Brazilian eastern Amazon. Seventy-t [...] wo samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from Santarém municipality were collected. Serum samples were submitted to Indirect Immunofluorescence Assay (IFA) with antigens of Babesia canis vogeli, Ehrlichia canis, and six Rickettsia species. The frequency of dogs containing anti-B. canis vogeli, anti-E. canis, and anti-Rickettsia spp. antibodies was 42.6%, 16.2%, and 31.7%, respectively. Anti-B. canis vogeli antibodies were detected in 59.6% of the urban dogs, and in 29.1% of the rural dogs (P < 0.05). For E. canis, seroprevalence was similar among urban (15.7%) and rural (16.6%) dogs. For Rickettsia spp., rural dogs presented significantly higher (P < 0.05) prevalence (40.3%) than urban animals (21.1%). This first study on tick-borne pathogens in dogs from the Brazilian eastern Amazon indicates that dogs are exposed to several agents, such as Babesia organisms, mostly in the urban area; Spotted Fever group Rickettsia organisms, mostly in the rural area; and Ehrlichia organisms, in dogs from both areas studied.

Spolidorio, Mariana Granziera; Minervino, Antonio Humberto Hamad; Valadas, Samantha Yuri Oshiro Branco; Soares, Herbert Sousa; Neves, Kedson Alessandri Lobo; Labruna, Marcelo Bahia; Ribeiro, Mucio Flavio Barbosa; Gennari, Solange Maria.

292

Guías Latinoamericanas de la RIICER para el diagnóstico de las rickettsiosis transmitidas por garrapatas / Latinamerican guidelines of RIICER for diagnosis of tick-borne rickettsioses  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Las rickettsiosis transmitidas por garrapatas son afecciones de distribución mundial, que por diferentes motivos se pueden considerar emergentes y reemergentes. Hasta hace escasos años la única rickettsiosis transmitida por garrapatas en Latinoamérica era la infección por Rickettsia rickettsii, pero [...] en la actualidad y fundamentalmente, gracias a la incorporación de nuevas herramientas para el diagnóstico microbiológico como la reacción en cadena de la polimerasa y secuenciación o el cultivo celular rápido en tubo cerrado, se han descrito e involucrado otras especies de Rickettsia en la producción de patología humana. En estas guías se detallan y describen las diferentes técnicas utilizadas para el diagnóstico microbiológico de las rickettsiosis. Además, se incluye una sección en la que se detallan las especies más importantes de garrapatas duras relacionadas con las rickettsiosis en Latinoamérica, con claves para su clasificación taxonómica. Abstract in english Tick-borne rickettsioses are worldwide infectious diseases that are considered emerging and re-emerging. Until recently the only tick-borne rickettsiosis present in Latin America was Rickettsia rickettsii infection, but to date, with the incorporation of new tools as PCR and sequencing and the quick [...] cellular close tube cultures (Shell-vial), new species has been involved as human pathogens. In these guidelines, we offer an update of the microbiological assays for diagnosing rickettsioses. Besides we have included a section in which the most important hard ticks involved in human rickettsioses in Latinoamerica are detailed.

José A, Oteo; Santiago, Nava; Rita de, Sousa; Salim, Mattar; José M, Venzal; Katia, Abarca; Marcelo B, Labruna; Jorge, Zavala-Castro.

293

Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever  

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Full Text Available Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF. This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi, Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.

KA Oliveira

2008-03-01

294

Absence of antibodies to Rickettsia spp., Bartonella spp., Ehrlichia spp. and Coxiella burnetii in Tahiti, French Polynesia  

Science.gov (United States)

Abtract Background In the Pacific islands countries and territories, very little is known about the incidence of infectious diseases due to zoonotic pathogens. To our knowledge, human infections due to Rickettsia spp., Coxiella burnetii, Ehrlichia spp. and Bartonella spp. have never been reported in French Polynesia; and infections due to C. burnetti have been reported worldwide except in New Zealand. To evaluate the prevalence of this disease, we conducted a serosurvey among French Polynesian blood donors. Methods The presence of immunoglobulin G antibodies against R. felis, R. typhi, R. conorii, C. burnetii, B. henselae, B. quintana, and E. chaffeensis was evaluated by indirect immunofluorescence assay in sera from 472 French Polynesian blood donors collected from 2011 to 2013. In addition, 178 ticks and 36 cat fleas collected in French Polynesia were also collected and tested by polymerase chain reaction to detect Rickettsia spp., B. henselae and Ehrlichia spp. Results None of the blood donors had antibodies at a significant level against Rickettsia spp., Coxiella burnetii, Ehrlichia spp. and Bartonella spp. All tested ticks and cat fleas were PCR-negative for Rickettsia spp., B. henselae, and Ehrlichia spp. Conclusion We cannot conclude that these pathogens are absent in French Polynesia but, if present, their prevalence is probably very low. C. burnetii has been reported worldwide except in New Zealand. It may also be absent from French Polynesia.

2014-01-01

295

Pathogenic Rickettsia species acquire vitronectin from human serum to promote resistance to complement-mediated killing.  

Science.gov (United States)

Bacteria of the genus Rickettsia are transmitted from arthropod vectors and primarily infect cells of the mammalian endothelial system. Throughout this infectious cycle, the bacteria are exposed to the deleterious effects of serum complement. Using Rickettsia conorii, the etiologic agent of Mediterranean spotted fever (MSF), as a model rickettsial species, we have previously demonstrated that this class of pathogen interacts with human factor H to mediate partial survival in human serum. Herein, we demonstrate that R.?conorii also interacts with the terminal complement complex inhibitor vitronectin (Vn). We further demonstrate that an evolutionarily conserved rickettsial antigen, Adr1/RC1281, interacts with human vitronectin and is sufficient to mediate resistance to serum killing when expressed at the outer-membrane of serum sensitive Escherichia coli. Adr1 is an integral outer-membrane protein whose structure is predicted to contain eight membrane-embedded ?-strands and four 'loop' regions that are exposed to extracellular milieu. Site-directed mutagenesis of Adr1 revealed that at least two predicted 'loop' regions are required to mediate resistance to complement-mediatedkilling and vitronectin acquisition. These results demonstrate that rickettsial species have evolved multiple mechanisms to evade complement deposition and that evasion of killing in serum is an evolutionarily conserved virulence attribute for this genus of obligate intracellular pathogens. PMID:24286496

Riley, Sean P; Patterson, Jennifer L; Nava, Samantha; Martinez, Juan J

2014-06-01

296

Isolation and characterization of the dnaA gene of Rickettsia prowazekii  

International Nuclear Information System (INIS)

The dnaA gene encoding the initiator protein of DNA replication was isolated from the obligate intracellular bacterium, Rickettsia prowazekii. Comparison of the deduced amino acid sequence of R. prowazekii DnaA with other bacterial DnaA proteins revealed extensive similarity. However, the rickettsial sequence is unique in the number of basic lysine residues found within a highly conserved portion of the putative DNA binding region, suggesting that the rickettsial protein may recognize a DNA sequence that differs from the consensus DnaA box sequence identified in other bacteria. Consensus DnaA box sequences, found upstream of many bacterial dnaA genes, were not identified upstream of rickettsial dnaA gene. In addition, gene organization within this region differed from that of other bacteria. The putative start of transcription of the rickettsial dnaA gene was localized to a site 522 nucleotides upstream of the DnaA start codon. Key words: Rickettsia prowazekii; dnaA gene; initiator protein (authors)

1998-04-01

297

Protein interaction mapping on a functional shotgun sequence of Rickettsia sibirica  

Science.gov (United States)

Protein interaction maps can reveal novel pathways and functional complexes, allowing ‘guilt by association’ annotation of uncharacterized proteins. To address the need for large-scale protein interaction analyses, a bacterial two-hybrid system was coupled with a whole genome shotgun sequencing approach for microbial genome analysis. We report the first large-scale proteomics study using this system, integrating de novo genome sequencing with functional interaction mapping and annotation in a high-throughput format. We apply the approach by shotgun sequencing and annotating the genome of Rickettsia sibirica strain 246, an obligate intracellular human pathogen among the Spotted Fever Group rickettsiae. The bacteria invade endothelial cells and cause lysis after large amounts of progeny have accumulated. Little is known about specific Rickettsial virulence factors and their mode of pathogenicity. Analysis of the combined genomic sequence and protein–protein interaction data for a set of virulence related Type IV secretion system (T4SS) proteins revealed over 250 interactions and will provide insight into the mechanism of Rickettsial pathogenicity.

Malek, Joel A.; Wierzbowski, Jamey M.; Tao, Wei; Bosak, Stephanie A.; Saranga, David J.; Doucette-Stamm, Lynn; Smith, Douglas R.; McEwan, Paul J.; McKernan, Kevin J.

2004-01-01

298

Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism.  

Science.gov (United States)

Sca2 (surface cell antigen 2) is the only bacterial protein known to promote both actin filament nucleation and profilin-dependent elongation, mimicking eukaryotic formins to assemble actin comet tails for Rickettsia motility. We show that Sca2's functional mimicry of formins is achieved through a unique mechanism. Unlike formins, Sca2 is monomeric, but has N- and C-terminal repeat domains (NRD and CRD) that interact with each other for processive barbed-end elongation. The crystal structure of NRD reveals a previously undescribed fold, consisting of helix-loop-helix repeats arranged into an overall crescent shape. CRD is predicted to share this fold and might form together with NRD, a doughnut-shaped formin-like structure. In between NRD and CRD, proline-rich sequences mediate the incorporation of profilin-actin for elongation, and WASP-homology 2 (WH2) domains recruit actin monomers for nucleation. Sca2's ?-helical fold is unusual among Gram-negative autotransporters, which overwhelmingly fold as ?-solenoids. Rickettsia has therefore "rediscovered" formin-like actin nucleation and elongation. PMID:23818602

Madasu, Yadaiah; Suarez, Cristian; Kast, David J; Kovar, David R; Dominguez, Roberto

2013-07-16

299

Effects of homologous and heterologous immunization on the reservoir competence of domestic dogs for Rickettsia conorii (israelensis).  

Science.gov (United States)

A number of spotted fever group (SFG) rickettsiae cause serious infections in humans. Several antigenically related rickettsial agents may coexist within the same geographical area, and humans or vertebrate hosts may be sequentially exposed to multiple SFG agents. We assessed whether exposure of a vertebrate reservoir to one SFG Rickettsia will affect the host's immune response to a related pathogen and the efficiency of transmission to uninfected ticks. Two pairs of dogs were each infected with either Rickettsia massiliae or Rickettsia conorii israelensis, and their immune response was monitored twice weekly by IFA. The four immunized dogs and a pair of naïve dogs were each challenged with R. conorii israelensis-infected Rhipicephalus sanguineus nymphs. Uninfected Rh. sanguineus larvae were acquisition-fed on the dogs on days 1, 7, and 14 post-challenge. These ticks were tested for the presence of rickettsial DNA after molting to the nymphal stage. The naive dogs became infected with R. conorii israelensis and were infectious to ticks for at least 3 weeks, whereas reservoir competence of dogs previously infected with either R. massiliae or R. conorii was significantly diminished. This opens an opportunity for decreasing the efficiency of transmission and propagation of pathogenic Rickettsia in natural foci by immunizing the primary hosts with closely related nonpathogenic SFG bacteria. However, neither homologous immunization nor cross-immunization significantly affected the efficiency of R. conorii transmission between cofeeding infected nymphs and uninfected larvae. At high densities of ticks, the efficiency of cofeeding transmission may be sufficient for yearly amplification and persistent circulation of a rickettsial pathogen in the vector population. PMID:24201056

Levin, M L; Zemtsova, G E; Montgomery, M; Killmaster, L F

2014-02-01

300

Rickettsia in Synanthropic and Domestic Animals and Their Hosts from Two Areas of Low Endemicity for Brazilian Spotted Fever in the Eastern Region of Minas Gerais, Brazil  

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The aim of this study was to understand the current epidemiology of rickettsial diseases in two rickettsial-endemic regions in Brazil. In the municipalities of Pingo D'Agua and Santa Cruz do Escalvado, among serum samples obtained from horses and dogs, reactivity by immunofluorescent assay against spotted fever group rickettsiae was verified. In some serum samples from opossums (Didelphis aurita) captured in Santa Cruz do Escalvado, serologic response against rickettsiae was also verified. Po...

2010-01-01

 
 
 
 
301

Prevalence and genetic analysis of Anaplasma phagocytophilum and Spotted Fever Group rickettsiae in the tick Ixodes ricinus in urban and periurban sites in southern Germany  

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In recent years, Anaplasma phagocytophilum and Rickettsia spp. have been detected in Ixodes ricinus in Germany and a focal distribution has been suggested for A. phagocytophilum. In the present study the prevalence of A. phagocytophilum and spotted fever group (SFG) rickettsiae was investigated in I. ricinus. DNA-extracts were taken from 2,862 unfed I. ricinus ticks (adults and nymphs) from eight sites in Munich, sampled over a five-month period. Single samples from three comparative sites ou...

Silaghi, Cornelia

2008-01-01

302

Serosurvey of antibodies against spotted fever group Rickettsia spp. in horse farms in Northern Paraná, Brazil / Soroprevalência de anticorpos contra Rickettsia spp. do grupo febre maculosa em equinos de haras no Norte do Paraná, Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Febre Maculosa Brasileira (FMB) é uma doença emergente, sendo Rickettsia rickettsii o seu principal agente etiológico. O objetivo deste estudo foi determinar a soroprevalência de rickettsia do grupo da febre maculosa em equinos de seis haras localizados nos municípios de Cambé, Santa Fé, Guaraci e L [...] ondrina. As propriedades eram localizadas na região Norte do Paraná onde casos de FMB ainda não foram diagnosticados. Foram colhidas amostras de sangue de 273 equinos, e os soros foram testados pela RIFI, usando R. rickettsii e R. parkeri como antígenos, considerando-se como positivos títulos >64. Entre 273 soros, 15 (5,5%) reagiram contra R. rickettsii e 5 (1,8%) para R. parkeri. Cinco de seis haras estudados tinham animais reativos, e a taxa de sororreatividade variou de 0 a 13%. Os títulos variaram de 64 para 512, e três amostras apresentaram título de 512. Nove animais reagiram para R. rickettsii com títulos quatro vezes maiores que para R. parkeri. Esses resultados sugerem que equinos no Norte do Estado do Paraná, Brasil, podem ter sido expostos a uma rickettsia idêntica ou muito próxima a R. rickettsii. Abstract in english Brazilian spotted fever (BSF) is an emerging disease most likely caused by Rickettsia rickettsii. The objective of the present study was to estimate the seroprevalence of BSF rickettsia infections in equines from six horse farms located in Londrina County, Paraná, Southern Brazil. Six owners of hors [...] e farms situated in Cambé, Santa Fé, Guaraci and Londrina municipalities participated in the study. All farms were located in areas where BSF has not been reported. A total of 273 horses were sampled and their sera were tested by indirect Immunofluorescence assay (IFA) using R. rickettsii and R. parkeri antigens. Titers equal to and greater than 64 were considered positive. Of 273 sera tested, 15 (5.5%) reacted to R. rickettsii and 5 (1.8%) to R. parkeri. Five out of the six farms studied revealed seropositive animals and seropositivity rate ranged from 0 to 13%. The titers ranged from 64 to 512, and four samples had a titer of 512. Nine animals reacted to R. rickettsii with titers four-fold higher than those for R. parkeri. These results suggest that horses in Northern Paraná may have been exposed to rickettsiae identical or closely related to R. rickettsii.

Tamekuni, Katia; Toledo, Roberta dos Santos; Silva Filho, Mauro de Freitas; Haydu, Valeska Bender; Pacheco, Richard Campos; Cavicchioli, José Henrique; Labruna, Marcelo Bahia; Dumler, John Stephen; Vidotto, Odilon.

303

Serosurvey of antibodies against spotted fever group Rickettsia spp. in horse farms in Northern Paraná, Brazil Soroprevalência de anticorpos contra Rickettsia spp. do grupo febre maculosa em equinos de haras no Norte do Paraná, Brasil  

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Full Text Available Brazilian spotted fever (BSF is an emerging disease most likely caused by Rickettsia rickettsii. The objective of the present study was to estimate the seroprevalence of BSF rickettsia infections in equines from six horse farms located in Londrina County, Paraná, Southern Brazil. Six owners of horse farms situated in Cambé, Santa Fé, Guaraci and Londrina municipalities participated in the study. All farms were located in areas where BSF has not been reported. A total of 273 horses were sampled and their sera were tested by indirect Immunofluorescence assay (IFA using R. rickettsii and R. parkeri antigens. Titers equal to and greater than 64 were considered positive. Of 273 sera tested, 15 (5.5% reacted to R. rickettsii and 5 (1.8% to R. parkeri. Five out of the six farms studied revealed seropositive animals and seropositivity rate ranged from 0 to 13%. The titers ranged from 64 to 512, and four samples had a titer of 512. Nine animals reacted to R. rickettsii with titers four-fold higher than those for R. parkeri. These results suggest that horses in Northern Paraná may have been exposed to rickettsiae identical or closely related to R. rickettsii.Febre Maculosa Brasileira (FMB é uma doença emergente, sendo Rickettsia rickettsii o seu principal agente etiológico. O objetivo deste estudo foi determinar a soroprevalência de rickettsia do grupo da febre maculosa em equinos de seis haras localizados nos municípios de Cambé, Santa Fé, Guaraci e Londrina. As propriedades eram localizadas na região Norte do Paraná onde casos de FMB ainda não foram diagnosticados. Foram colhidas amostras de sangue de 273 equinos, e os soros foram testados pela RIFI, usando R. rickettsii e R. parkeri como antígenos, considerando-se como positivos títulos >64. Entre 273 soros, 15 (5,5% reagiram contra R. rickettsii e 5 (1,8% para R. parkeri. Cinco de seis haras estudados tinham animais reativos, e a taxa de sororreatividade variou de 0 a 13%. Os títulos variaram de 64 para 512, e três amostras apresentaram título de 512. Nove animais reagiram para R. rickettsii com títulos quatro vezes maiores que para R. parkeri. Esses resultados sugerem que equinos no Norte do Estado do Paraná, Brasil, podem ter sido expostos a uma rickettsia idêntica ou muito próxima a R. rickettsii.

Katia Tamekuni

2010-12-01

304

Molecular detection of spotted fever group rickettsiae associated with ixodid ticks in Egypt.  

Science.gov (United States)

Tick-borne diseases comprise a complex epidemiological and ecological network that connects the vectors, pathogens, and a group of host species. The aim of this study was to identify bacteria from the genus Rickettsia associated with ixodid ticks infesting camels and cows in Egypt. Ticks were collected from 6 different localities: Qina, Giza, Qalet El Nakhl, New Valley, El Arish, and Minufia, from July to October 2008. Species were identified using PCR, followed by sequencing. The gltA and rOmpA genes were used for the initial detection of Rickettsia spp. Further characterization of positive samples utilized primers targeting rOmpB, sca4, and intergenic spacers (mppA-purC, dksA-xerC, and rpmE-tRNA(fMet)). Cows were infested with Hyalomma anatolicum excavatum and Boophilus annulatus. Camels were infested with Hyalomma dromedarii, H. impeltatum, and H. marginatum marginatum. Approximately 57.1% of H. dromedarii ticks collected from Qalet El Nakhl were infected with Rickettsia africae, exhibiting 99.1-100% identity to reference strains. Within H. impeltatum, 26.7% and 73.3% of ticks from El Arish were infected with R. africae and R. aeschlimannii, with 98.3-100% and 97.9-100% identity, respectively. Furthermore, 33.3% of H. marginatum marginatum ticks in Qalet El Nakhl were infected with the same two species as H. impeltatum, demonstrating 99.1-100% and 99.3-100% identity, respectively. By comparing percent identities and phylogenetic relationships, R. africae is identified for the first time in Egypt, in addition to R. aeschlimannii, which exhibits 100% identity with the Stavropol strain in GenBank. In conclusion, the obtained data underscore the medical and veterinary importance of tick-borne rickettsioses, which necessitate further investigation by authorities in Egypt. Moreover, additional characterization of these rickettsial isolates should be performed to designate their strains, using a polyphasic strategy combining genotypic and phenotypic tests, to facilitate their deposition in the rickettsial collection of the WHO and/or ATCC. PMID:22217182

Abdel-Shafy, Sobhy; Allam, Nesreen A T; Mediannikov, Oleg; Parola, Philippe; Raoult, Didier

2012-05-01

305

Antibodies to Rickettsia rickettsii, Rickettsia typhi, Coxiella burnetii, Bartonella henselae, Bartonella quintana, and Ehrlichia chaffeensis among healthy population in Minas Gerais, Brazil  

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Full Text Available SciELO Brazil | Language: English Abstract in english Rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in South America particularly in Brazil where few epidemiological reports have been published. We describe a serosurvey for Rickettsia rickettsii, R. typhi, Coxiella burnetii, Bartonella henselae, B. [...] quintana, and Ehrlichia chaffeensis in 437 healthy people from a Brazilian rural community. The serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 was used. The seroprevalence rates for R. rickettsii, R. typhi, C. burnetii, B. henselae, B. quintana, and E. chaffeensis were respectively 1.6% (7 samples); 1.1% (5 samples); 3.9% (17 samples); 13.7% (60 samples); 12.8% (56 samples), and 10.5% (46 samples). Frequent multiple/cross-reactivity was observed in this study. Age over 40 years old, urban profession, and rural residence were significantly associated with some but not all infections rate. Low seropositivity rates for R. rickettsii, R. typhi, and C. burnetii contrasted with higher rates of seropositivity for B. quintana, B. henselae, and E. chaffeensis. These results show that all tested rickettsial species or antigenically closely related possible exist in this particular region.

Costa, Paulo Sérgio Gonçalves da; Brigatte, Marcos Emilio; Greco, Dirceu Bartolomeu.

306

Antibodies to Rickettsia rickettsii, Rickettsia typhi, Coxiella burnetii, Bartonella henselae, Bartonella quintana, and Ehrlichia chaffeensis among healthy population in Minas Gerais, Brazil  

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Full Text Available Rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in South America particularly in Brazil where few epidemiological reports have been published. We describe a serosurvey for Rickettsia rickettsii, R. typhi, Coxiella burnetii, Bartonella henselae, B. quintana, and Ehrlichia chaffeensis in 437 healthy people from a Brazilian rural community. The serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 was used. The seroprevalence rates for R. rickettsii, R. typhi, C. burnetii, B. henselae, B. quintana, and E. chaffeensis were respectively 1.6% (7 samples; 1.1% (5 samples; 3.9% (17 samples; 13.7% (60 samples; 12.8% (56 samples, and 10.5% (46 samples. Frequent multiple/cross-reactivity was observed in this study. Age over 40 years old, urban profession, and rural residence were significantly associated with some but not all infections rate. Low seropositivity rates for R. rickettsii, R. typhi, and C. burnetii contrasted with higher rates of seropositivity for B. quintana, B. henselae, and E. chaffeensis. These results show that all tested rickettsial species or antigenically closely related possible exist in this particular region.

Paulo Sérgio Gonçalves da Costa

2005-12-01

307

Candidatus Rickettsia andeanae, a spotted fever group agent infecting Amblyomma parvum ticks in two Brazilian biomes.  

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Adult ticks of the species Amblyomma parvum were collected from the vegetation in the Pantanal biome (state of Mato Grosso do Sul) and from horses in the Cerrado biome (state of Piauí) in Brazil. The ticks were individually tested for rickettsial infection via polymerase chain reaction (PCR) targeting three rickettsial genes, gltA, ompA and ompB. Overall, 63.5% (40/63) and 66.7% (2/3) of A. parvum ticks from Pantanal and Cerrado, respectively, contained rickettsial DNA, which were all confirmed by DNA sequencing to be 100% identical to the corresponding fragments of the gltA, ompA and ompB genes of Candidatus Rickettsia andeanae. This report is the first to describe Ca. R. andeanae in Brazil. PMID:24714968

Nieri-Bastos, Fernanda Aparecida; Lopes, Marcos Gomes; Cançado, Paulo Henrique Duarte; Rossa, Giselle Ayres Razera; Faccini, João Luiz Horácio; Gennari, Solange Maria; Labruna, Marcelo Bahia

2014-04-01

308

Characterisation of an emerging rickettsia-like organism in Tasmanian farmed Atlantic salmon Salmo salar.  

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A rickettsia-like organism (RLO) was observed in farmed Atlantic salmon Salmo salar located in south-east Tasmania, Australia. Several assays such as immunoperoxidase, immunoelectron microscopy, polymerase chain reaction and nucleic acid sequencing, as well as phylogenetic analysis of rDNA sequences, were performed on infected fish tissues. Immunohistochemistry results suggested the presence of related antigenic determinants between the Tasmanian RLO and the type strain LF-89 of Piscirickettsia salmonis. However, sequence alignment demonstrated that the Tasmanian RLO contains a 19 bp deletion at the 3'-end of the internal transcribed spacer region of the rDNA operon, indicating a genetic divergence from P. salmonis isolates, which are exotic to Australia. PMID:15900686

Corbeil, Serge; Hyatt, Alex D; Crane, Mark St J

2005-04-01

309

Featured Organism: Reductive Evolution in Bacteria: Buchnera sp., Rickettsia Prowazekii and Mycobacterium Leprae  

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Full Text Available Obligate intracellular bacteria commonly have much reduced genome sizes compared to their nearest free-living relatives. One reason for this is reductive evolution: the loss of genes rendered non-essential due to the intracellular habitat. This can occur because of the presence of orthologous genes in the host, combined with the ability of the bacteria to import the protein or metabolite products of the host genes. In this article we take a look at three such bacteria whose genomes have been fully sequenced. Buchnera is an endosymbiont of the pea aphid, Acyrthosiphon pisum, the relationship between these two organisms being so essential that neither can reproduce in the absence of the other. Rickettsia prowazekii is the causative agent of louse-borne typhus in humans and Mycobacterium leprae infection of humans leads to leprosy. Both of these human pathogens have fastidious growth requirements, which has made them very difficult to study.

Jo Wixon

2001-01-01

310

Identification of CD8 T-Lymphocyte Epitopes in OmpB of Rickettsia conorii  

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The 1.2-kb DNA fragment of the Rickettsia conorii outer membrane protein B gene (OmpB451-846) was subcloned using site-specific PCR primers and expressed as six smaller fragments: OmpB458-652, OmpB595-744, OmpB595-654, OmpB645-692, OmpB689-744, and OmpB739-848. NCTC cells transfected with a mammalian expression vector expressing the fragments OmpB689-744 and OmpB739-848 stimulated immune anti-R. conorii CD8 T lymphocytes, suggesting the presence of CD8 T-lymphocyte-stimulating epitopes on the...

2003-01-01

311

Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii strains.  

Science.gov (United States)

By use of the nearly perfectly colinear genomes of Rickettsia conorii and Rickettsia prowazekii, we compared the usefulness of three types of sequences for typing of R. conorii isolates: (i) 5 variable coding genes comprising the 16S ribosomal DNA, gltA, ompB, and sca4 (gene D) genes, which are present in both genomes, and the ompA gene, which is degraded in R. prowazekii; (ii) 28 genes degraded in R. conorii but intact in R. prowazekii, including 23 split and 5 remnant genes; and (iii) 27 conserved and 25 variable intergenic spacers. The 4 conserved and 23 split genes as well as the 27 conserved intergenic spacers each had identical sequences in 34 human and 5 tick isolates of R. conorii. Analysis of the ompA sequences identified three genotypes of R. conorii. The variable intergenic spacers were significantly more variable than conserved genes, split genes, remnant genes, and conserved spacers (P mppA-purC, rpmE-tRNA(fMet), and tRNA(Gly)-tRNA(Tyr)) had highly variable sequences; when they were combined for typing, multispacer typing (MST) identified 27 different genotypes in the 39 R. conorii isolates. Two batches from the same R. conorii strain, Malish (Seven), with different culture passage histories were found to exhibit the same MST type. MST was more discriminatory for strain genotyping than multiple gene sequencing (P < 10(-2)). Phylogenetic analysis based on MST sequences was concordant with the geographic origins of R. conorii isolates. Our study supports the usefulness of MST for strain genotyping. This tool may be useful for tracing a strain and identifying its source during outbreaks, including those resulting from bioterrorism. PMID:15583310

Fournier, Pierre-Edouard; Zhu, Yong; Ogata, Hiroyuki; Raoult, Didier

2004-12-01

312

The Effects of Beta Rays (Tritium) on the Growth of Rickettsiae and Influenza Virus  

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The growth of Rickettsia mooseri (murine typhus) was increased greatly in embryonate eggs containing tritium oxide at levels of 180, 90 and 45 mc/egg and slightly in eggs containing 22.5 mc/egg; the infections in those given 11.2 mc/egg did not differ from the control. The growth of Rickettsia akari (rickettsial pox) was inhibited completely in eggs containing tritium oxide at levels of 180, 90 and 45 mc/egg, and partially in groups containing 22.5 and 11.2 mc/egg. Results similar to the above were obtained following the inoculation of tritium oxide, 1 mc per 108 cells, into monoclayer cultures of KB cells infected with R. mooseri or R. akari. A decrease in the latent period for the release of influenza virus from the chorioallantoic membranes of embryonate eggs followed the injection of tritium oxide prior to infection with virus. A thousand-fold increase of virus in the chorioallantoic fluids of treated eggs was found 2 h after the inoculation of the infective agent. When detectable amounts of virus appeared in the chorioallantoic fluids of the control eggs, 8 h after infection, the ID50 of the tritium-treated eggs was 10-5,6; both series had an ID50 of 10-7.5 24 h after infection. The amounts of virus in the membranes -of the tritium-treated eggs was increased also. This virus was liberated by removing and washing the membranes, drying the membranes by vacuum sublimation at low temperatures and rupturing the cells of the membranes by rehydration with distilled water. (author)

1962-02-01

313

Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted fever in the city of Rio de Janeiro, Brazil  

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Full Text Available A lethal case of Brazilian spotted fever (BSF is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA for spotted fever group rickettsia (SFGR was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR and the nucleotide sequencing of a fragment of the ompA gene showed 100% homology to Rickettsia rickettsii. BSF has not been reported in the city of Rio de Janeiro in the last three decades, and the present description should alert the clinicians to its presence in urban Rio de Janeiro, and to the differential diagnosis with dengue fever, gastroenteritis, leptospirosis and bacterial septic shock, among others.

Cristiane Lamas

2008-04-01

314

Chemical properties of lipopolysaccharides from spotted fever group rickettsiae and their common antigenicity with lipopolysaccharides from Proteus species.  

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The lipopolysaccharides (LPS) isolated from spotted fever group (SFG) rickettsia strains Thai tick typhus TT-118 and Katayama were characterized by chemical analyses, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme-linked immunosorbent assay (ELISA), and immunoblotting. These LPS did not contain heptose, but they contained 3-deoxy-D-manno-octulosonic acid (KDO), glucosamine, quinovosamine, phosphate, ribose, an unknown neutral sugar, and palmitic acid. Resolution of the appa...

1993-01-01

315

Rickettsia ‘In’ and ‘Out’: Two Different Localization Patterns of a Bacterial Symbiont in the Same Insect Species  

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Intracellular symbionts of arthropods have diverse influences on their hosts, and their functions generally appear to be associated with their localization within the host. The effect of localization pattern on the role of a particular symbiont cannot normally be tested since the localization pattern within hosts is generally invariant. However, in Israel, the secondary symbiont Rickettsia is unusual in that it presents two distinct localization patterns throughout development and adulthood i...

2011-01-01

316

Bartonella and Rickettsia from fleas (Siphonaptera: Ceratophyllidae) of prairie dogs (Cynomys spp.) from the western United States.  

Science.gov (United States)

Fleas of prairie dogs have been implicated in the transmission of Bartonella spp. We used PCR to test DNA extracts from 47 fleas of prairie dogs from 6 states. We amplified DNA from 5 unique genotypes of Bartonella spp. and 1 Rickettsia sp. from 12 fleas collected in North Dakota, Oklahoma, Texas, and Wyoming. Sequences from the Bartonella spp. were similar, but not identical, to those from prairie dogs and their fleas in Colorado. PMID:17918386

Reeves, Will K; Rogers, Thomas E; Dasch, Gregory A

2007-08-01

317

Crystal structure of the DNA-bound VapBC2 antitoxin/toxin pair from Rickettsia felis  

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Besides their commonly attributed role in the maintenance of low-copy number plasmids, toxin/antitoxin (TA) loci, also called ‘addiction modules’, have been found in chromosomes and associated to a number of biological functions such as: reduction of protein synthesis, gene regulation and retardation of cell growth under nutritional stress. The recent discovery of TA loci in obligatory intracellular species of the Rickettsia genus has prompted new research to establish whether they work a...

Mate?, Mari?a J.; Vincentelli, Renaud; Foos, Nicolas; Raoult, Didier; Cambillau, Christian; Ortiz-lombardi?a, Miguel

2012-01-01

318

First molecular evidence of Anaplasma ovis and Rickettsia spp. in keds (Diptera: Hippoboscidae) of sheep and wild ruminants.  

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To evaluate the presence of rickettsial agents in hippoboscid flies with molecular methods, 81 sheep keds (Melophagus ovinus) were collected from 23 sheep, 144 deer keds (Lipoptena cervi) were caught in the environment, and a further 463 and 59 individuals of the latter species were obtained from fresh carcasses of 29 red deer and 17 roe deer, respectively. DNA was extracted individually or in pools. Anaplasma ovis was demonstrated in all examined sheep keds, and from one pool of free-living deer keds. Rickettsia helvetica or other, unidentified rickettsiae were also present in one pool of sheep keds, and in four pools of deer keds from both red deer and roe deer. This is the first account of polymerase chain reaction positivity of hippoboscid flies for A. ovis and rickettsiae. These results raise the possibility that-apart from cattle and roe deer as already reported-sheep and red deer might also play a reservoir role in the epidemiology of rickettsioses. PMID:21923269

Hornok, Sándor; de la Fuente, José; Biró, Nóra; Fernández de Mera, Isabel G; Meli, Marina L; Elek, Vilmos; Gönczi, Eniko; Meili, Theres; Tánczos, Balázs; Farkas, Róbert; Lutz, Hans; Hofmann-Lehmann, Regina

2011-10-01

319

A new Rickettsia species found in fleas collected from human dwellings and from domestic cats and dogs in Senegal.  

Science.gov (United States)

The insects of the order Siphonaptera, commonly named fleas, are vectors of pathogens around the world. Our previous studies showed that 4.4% of acute febrile diseases in the Sine-Saloum region of Senegal were due to Rickettsia felis. The aim of this study was to explain the high prevalence of R. felis infections in two rural Senegalese populations by an entomological, systematic monitoring protocol. A total of 232 fleas from three species (Ctenocephalides felis, Echidnophaga gallinacea, and Synosternus pallidus) were collected by candle trapping and manually from pets in the villages of Dielmo and Ndiop during the year 2010. The fleas were then tested for the presence of Bartonella and Rickettsia species. No fleas were found to be positive for any Bartonella species or R. felis. Surprisingly, we found that 91.4% of S. pallidus were infected by a new Rickettsia species, which, based on sequence analysis of gltA, ompB, and two fragments of rpoB, was found to be closely related to R. felis. The results from this study did not explain the high incidence of R. felis infections in these Senegalese populations. PMID:22214272

Roucher, Clementine; Mediannikov, Oleg; Diatta, Georges; Trape, Jean-François; Raoult, Didier

2012-05-01

320

Molecular characterization of Rickettsia massiliae and Anaplasma platys infecting Rhipicephalus sanguineus ticks and domestic dogs, Buenos Aires (Argentina).  

Science.gov (United States)

Rickettsioses, ehrlichioses and anaplasmoses are emerging diseases that are mainly transmitted by arthropods and that affect humans and animals. The aim of the present study was to use molecular techniques to detect and characterize those pathogens in dogs and ticks from Buenos Aires city. We studied 207 Rhipicephalus sanguineus ticks and 52 canine blood samples from poor neighborhoods of Buenos Aires city. The samples were molecularly screened for the genera Rickettsia, Ehrlichia, and Anaplasma by PCR and sequencing. DNA of Rickettsia massiliae (3.4%) and Anaplasma platys (13.5%) was detected in ticks and blood samples, respectively. For characterization, the positive samples were subjected to amplification of a fragment of the 190-kDa outer membrane protein gene (spotted fever group rickettsiae) and a fragment of the groESL gene (specific for A. platys). A phylogenetic tree was constructed using the neighbor-joining method, revealing that the sequences were closely related to those of strains from other geographic regions. The results indicate that human and animal pathogens are abundant in dogs and their ticks in Buenos Aires city and portray the potentially high risk of human exposure to infection with these agents, especially in poor neighborhoods, where there is close contact with animals in an environment of poor health conditions. PMID:24907186

Cicuttin, Gabriel L; Brambati, Diego F; Rodríguez Eugui, Juan I; Lebrero, Cecilia González; De Salvo, María N; Beltrán, Fernando J; Gury Dohmen, Federico E; Jado, Isabel; Anda, Pedro

2014-09-01

 
 
 
 
321

Rickettsia felis (Rickettsiales: Rickettsiaceae in Ctenocephalides felis felis (Siphonaptera: Pulicidae in the State of São Paulo, Brazil Rickettsia felis (Rickettsiales: Rickettsiaceae em Ctenocephalides felis felis (Siphonaptera: Pulicidae no estado de São Paulo  

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Full Text Available Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90% and four fleas from Mogi das Cruzes (28% were positive for the 17-kDa gene, and eight fleas from Pedreira (80% and four from Mogi das Cruzes (28% were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to the GenBank Data, they were 100% identical to the 190-kDa sequence of R. felis. This was the first report of its occurrence in the State of São Paulo.Amostras de 10 e 14 pulgas Ctenocephalides felis felis foram coletadas de cães nos municípios de Pedreira e Mogi das Cruzes, respectivamente, no estado de São Paulo, para pesquisa de Rickettsia spp. As pulgas foram individualmente submetidas à reação em cadeia pela polimerase, tendo como alvo os genes 17-kDa e 190-kDa (OmpA de Rickettsia, sendo esse último específico para o GFM. Nove pulgas de Pedreira (90% e quatro pulgas de Mogi das Cruzes (28% foram positivas para o gene 17-kDa, e oito pulgas de Pedreira (80% e quatro de Mogi das Cruzes (28% foram positivas para o gene 190-kDa. As seqüências de nucleotídeos do gene 190-kDa de uma pulga de Pedreira e de uma pulga de Mogi das Cruzes foram 100% idênticas; quando comparadas com dados existentes no GenBank, foram 100% idênticas com a seqüência parcial do gene 190-kDa de Rickettsia felis. Esse foi o primeiro relato de sua ocorrência no estado de São Paulo.

M.C. Horta

2005-06-01

322

High prevalence of spotted fever group rickettsiae in Amblyomma variegatum from Uganda and their identification using sizes of intergenic spacers.  

Science.gov (United States)

The spotted fever group (SFG) rickettsiae are obligate intracellular bacteria transmitted by ticks that cause several tick-borne rickettsioses in humans worldwide. This study was intended to determine the prevalence of SFG rickettsiae in Amblyomma variegatum from 7 districts across Uganda. In addition to sequencing of gltA and ompA genes, identification of Rickettsia species based on the sizes of highly variable intergenic spacers, namely, dksA-xerC, mppA-purC, and rpmE-tRNA(fMet) was carried out. Application of multiplex PCR for simultaneous amplification of 3 spacers combined with capillary electrophoresis separation allowed simple, accurate, and high-throughput fragment sizing with considerable time and cost savings. Rickettsia genus-specific real-time PCR detected 136 positives out of 140 samples, giving an overall prevalence of 97.1%. Most samples (n=113) had a size combination of 225, 195, and 341 bp for dksA-xerC, mppA-purC, and rpmE-tRNA(fMet), respectively, which was identical to that of R. africae, a causative agent of African tick bite fever. In addition, several samples had size variants in either dksA-xerC or rpmE-tRNA(fMet). Nonetheless, the partial sequences of gltA and ompA genes of samples of all size combinations showed the greatest similarity to R. africae (99.3-100% for gltA and 98.1-100% for ompA). Given these results, it is highly possible that the tested ticks were infected with R. africae or closely related species. This is a first report on molecular genetic detection of R. africae and its high endemicity in Uganda. Clinicians in this country should be aware of this pathogen as a cause of non-malarial febrile illness. This study provided a starting point for the development of Rickettsia species identification based on the sizes of intergenic spacers. The procedure is simple, rapid, and cost-effective to perform; hence it might be particularly well suited for preliminary species identification in epidemiological investigations. The results may be more detailed and reliable when simultaneous sequencing analysis is performed. PMID:24331642

Nakao, Ryo; Qiu, Yongjin; Igarashi, Manabu; Magona, Joseph W; Zhou, Lijia; Ito, Kimihito; Sugimoto, Chihiro

2013-12-01

323

Spotted fever group Rickettsia in small rodents from areas of low endemicity for Brazilian spotted fever in the eastern region of Minas Gerais State, Brazil.  

Science.gov (United States)

We investigated the humoral immune response against different species of Rickettsia in serum samples from small rodents collected in two areas of a silent focus for Brazilian spotted fever in the eastern region of Minas Gerais State, Brazil. Sera samples were analyzed by indirect immunofluorescence assay using antigens from Rickettsia species of the spotted fever, ancestral, and transition groups. Titers ? 1:64 were considered positive. In Santa Cruz do Escalvado, 94% (30 of 32) of the samples collected from Rattus rattus, 22% (5 of 23) from Nectomys squamipes, and 80% (4 of 5) from Akodon sp., reacted by indirect immunofluorescence assay with Rickettsia antigens of the spotted fever group. In the municipality of Pingo D'Água, 84% (26 of 31) of the samples collected from R. rattus, 86% (6 of 7) of the samples from Oryzomys subflavus, 86% (6 of 7) from N. squamipes, and 100% (1 of 1) from Bolomys sp. contained antibodies that reacted with rickettsial antigens of the spotted fever group. These results demonstrated the previous exposure of small rodents to spotted fever group Rickettsia, suggesting the participation of these animals in the natural history of these rickettsiae in this region. PMID:23509125

Milagres, Bruno S; Padilha, Amanda F; Montandon, Carlos E; Freitas, Renata N; Pacheco, Richard; Walker, David H; Labruna, Marcelo B; Mafra, Cláudio L; Galvão, Márcio A M

2013-05-01

324

Susceptibility of Rickettsia conorii, R. rickettsii, and Coxiella burnetii to PD 127,391, PD 131,628, pefloxacin, ofloxacin, and ciprofloxacin.  

Science.gov (United States)

Plaque formation and dye uptake assays were used to measure the MICs of PD 127,391 and PD 131,628 against Rickettsia species. The MICs of PD 127,391 were 0.25 microgram/ml for Rickettsia rickettsii and 0.125 to 0.25 microgram/ml for Rickettsia conorii. The MICs of PD 131,628 were 0.25 to 0.5 microgram/ml for R. rickettsii and 0.5 microgram/ml for R. conorii. As determined by the shell vial technique, 15 strains of Coxiella burnetii were susceptible to PD 127,391 and PD 131,628 (MIC, MP10) was of intermediate susceptibility. PMID:1336950

Jabarit-Aldighieri, N; Torres, H; Raoult, D

1992-11-01

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Distribution of Rickettsia rickettsii in ovary cells of Rhipicephalus sanguineus (Latreille1806 (Acari: Ixodidae  

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Full Text Available Abstract Background Considering the fact that the dog tick, Rhipicephalus sanguineus, has a great potential to become the vector of Brazilian Spotted Fever (BSF for humans, the present study aimed to describe the distribution of the bacterium Rickettsia rickettsii, the etiological agent of BSF, in different regions of the ovaries of R. sanguineus using histological techniques. The ovaries were obtained from positive females confirmed by the hemolymph test and fed in the nymph stage on guinea pigs inoculated with R. rickettsii. Results The results showed a general distribution of R. rickettsii in the ovary cells, being found in oocytes in all stages of development (I, II, III, IV and V most commonly in the periphery of the oocyte and also in the cytoplasm of pedicel cells. Conclusions The histological analysis of the ovaries of R. sanguineus infected females confirmed the presence of the bacterium, indicating that the infection can interfere negatively in the process of reproduction of the ticks, once alterations were detected both in the shape and cell structure of the oocytes which contained bacteria.

da Silva Costa Luís

2011-11-01

326

Detection of Rickettsia japonica in Haemaphysalis longicornis ticks by restriction fragment length polymorphism of PCR product.  

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PCR was applied to the detection of Rickettsia japonica, the causative agent of Oriental spotted fever (OSF), in ticks collected at two sites of the Muroto area on Shikoku Island, a major area in Japan where OSF is endemic. Primer pair Rr190.70p and Rr190.602n of the R. rickettsii 190-kDa antigen gene sequence of Regnery and others (R.L. Regnery, C.L. Spruill, and B.D. Plikaytis, J. Bacteriol. 173:1576-1589, 1991) primed the DNA extracted from Haemaphysalis longicornis ticks but not those extracted from Haemaphysalis formosensis, Haemaphysalis flava, Haemaphysalis hystricis, or Amblyomma testudinarium ticks. Digestion of the amplification product with the restriction endonucleases PstI and AluI produced the restriction fragment length polymorphism pattern specific to R. japonica. The HindIII and MspI digests gave restriction fragment length polymorphism patterns identical to those of the PCR product from R. japonica DNA. Hemolymph preparations of H. longicornis ticks were demonstrated to contain rod-shaped organisms that were detected by immunofluorescence with the monoclonal antibody specific to R. japonica species. The primer pair did not amplify the DNA of a laboratory colony of H. longicornis ticks originally collected at an area where OSF is not endemic. Our results provided evidence that H. longicornis ticks might be an arthropod reservoir for R. japonica and a vector of OSF. PMID:7790445

Uchida, T; Yan, Y; Kitaoka, S

1995-04-01

327

Population analyses of Amblyomma maculatum ticks and Rickettsia parkeri using single-strand conformation polymorphism.  

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Gulf Coast ticks, Amblyomma maculatum, and the zoonotic agents they transmit, Rickettsia parkeri, are expanding into areas in the United States where they were not previously reported, and are emerging threats for public and veterinary health. The dynamics of this tick-pathogen system and implications for disease transmission are still unclear. To assess genetic variation of tick and rickettsial populations, we collected adult A. maculatum from 10 sites in Mississippi, 4 in the northern, one in the central, and 5 in the southern part of the state. PCR amplicons from tick mitochondrial 16S rRNA and rickettsial ompA genes as well as 5 intergenic spacer regions were evaluated for genetic variation using single-strand conformation polymorphism analysis. Frequencies of the 4 tick 16S haplotypes were not significantly different among regions of Mississippi, but within sites there were differences in distribution that can be explained by high migration rates. Phylogenetically, one lineage of tick haplotypes was a species-poor sister group to remaining haplotypes in the species-rich sister group. No genetic variation was identified in any of the 6 selected gene targets of R. parkeri examined in the infected ticks, suggesting high levels of intermixing. PMID:23856472

Ferrari, Flavia A G; Goddard, Jerome; Caprio, Michael; Paddock, Christopher D; Mixson-Hayden, Tonya; Varela-Stokes, Andrea S

2013-09-01

328

Molecular evidence of Ehrlichia canis and Rickettsia massiliae in ixodid ticks of carnivores from South Hungary.  

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To monitor the emergence of thermophilic, Mediterranean ixodid tick species and tick-borne pathogens in southern Hungary, 348 ticks were collected from shepherd dogs, red foxes and golden jackals during the summer of 2011. Golden jackals shared tick species with both the dog and the red fox in the region. Dermacentor nymphs were collected exclusively from dogs, and the sequence identification of these ticks indicated that dogs are preferred hosts of both D. reticulatus and D. marginatus nymphs, unlike previously reported. Subadults of three ixodid species were selected for reverse line blot hybridisation (RLB) analysis to screen their vector potential for 40 pathogens/groups. Results were negative for Anaplasma, Babesia and Theileria spp. Investigation of D. marginatus nymphs revealed the presence of Ehrlichia canis, Rickettsia massiliae and Borrelia afzelii for the first time in this tick species. These findings broaden the range of those tick-borne agents, which are typically transmitted by Rhipicephalus sanguineus, but may also have Dermacentor spp. as potential or alternative vectors. Ehrlichiacanis was also newly detected in Ixodes canisuga larvae from red foxes. In absence of transovarial transmission in ticks this implies that Eurasian red foxes may play a reservoir role in the epidemiology of canine ehrlichiosis. PMID:23439290

Hornok, Sándor; Fuente, José; Horváth, Gábor; Fernández de Mera, Isabel G; Wijnveld, Michiel; Tánczos, Balázs; Farkas, Róbert; Jongejan, Frans

2013-03-01

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La detección de Bartonella spp. y Rickettsia spp. en pulgas, garrapatas y piojos recolectados en las zonas rurales de Perú / Detection of Bartonella spp. and Rickettsia spp. in fleas, ticks and lice collected in rural areas of Peru  

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Full Text Available SciELO Peru | Language: Spanish Abstract in spanish La Bartonellosis y la Rickettsiosis son enfermedades comúnmente reportadas en Perú. Con el propósito de detectar Bartonella sp. y Rickettsia sp. especímenes de pulgas, garrapatas y piojos de cinco localidades del Perú (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro) fueron colectadas y an [...] alizadas. Para la detección se usó PCR y una posterior confirmación con secuenciamiento de DNA. Los especímenes colectados fueron agrupados en 102 pools (76 Ctenocephalides felis, dos Ctenocephalides canis, 16Pulex irritans, cinco Pediculus humanus, dos Rhiphicephalus sanguineus, y un Boophilus spp.). Bartonella fue detectada en 17 pools (seis de C. felis, nueve de P. irritans, uno de C. canis, y uno de P. humanus). Rickettsia fue detectada en 76 pools (62 de C. felis, 10 de P. irritans, dos de P. humanus, y dos de C. canis). Bartonella clarridgeiae fue detectada en C. felis (5.3% especímenes), C. canis (50%) y P. irritans (12.5%). Bartonella rochalimae fue detectada en C. felis (1.3%) y P. irritans (12.5%). Además, se detectó B. henselae en C. felis (1.3%) y P. humanus (20%). Bartonella spp. también se encontró en P. irritans (31,3%). Además, se detectó R. felis en C. felis (76.3%), C. canis (100%) y P. irritans (37.5%), y Rickettsia spp. se detectó en C. felis (5,3%), P. irritans (25%) y P. humanus (40%). Estos resultados demuestran la circulación de estas bacterias en el Perú Abstract in english Bartonellosis and rickettsiosis are commonly reported in Peru. In order to detect Bartonella sp. and Rickettsia sp. in fleas, ticks and lice, specimens from five distinct locations in Peru (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro) were collected and screened for the presence of the [...] se bacteria using PCR and later confirmation by DNA sequencing. The specimens collected were distributed in 102 pools (76 Ctenocephalides felis, 2 Ctenocephalides canis, 16Pulex irritans, 5 Pediculus humanus, 2 Rhiphicephalus sanguineus, and 1 Boophilus spp.), whereBartonella was detected in 17 pools (6 of C. felis, 9 of P. irritans, 1 of C. canis, and 1 P. humanus). Also, Rickettsia was detected in 76 pools (62 C. felis, 10 P. irritans, 2 P. humanus, and 2 C. canis). Bartonella clarridgeiae was detected in C. felis, C. canis and P. irritans pools at 5.3%, 50% and 12.5%, respectively. Bartonella rochalimae was detected in one C. felis and two P. irritans pools at 1.3% and 12.5%, respectively. Furthermore, B. henselae was detected in one C. felis pool and one P. humanus pool corresponding to 1.3% and 20%, respectively; andBartonella spp. was also found in 5 pools of P. irritans at 31.3%. Additionally, R. felis was detected in C. felis, C. canis and P. irritans pools at 76.3%, 100% and 37.5%, respectively; and Rickettsia spp. was detected in C. felis, P. irritans and P. humanus pools at 5.3%, 25% and 40%, respectively. These results demonstrate the circulation of these bacteria in Peru

Abraham G, Cáceres; Carlos P, Padilla Rojas; Javier, Arias Stella; Gerardo, Huatuco Crisanto; Antero, Gonzales Pérez.

330

Lawsonia intracellularis contains a gene encoding a functional rickettsia-like ATP/ADP translocase for host exploitation.  

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ATP/ADP translocases are a hallmark of obligate intracellular pathogens related to chlamydiae and rickettsiae. These proteins catalyze the highly specific exchange of bacterial ADP against host ATP and thus allow bacteria to exploit their hosts' energy pool, a process also referred to as energy parasitism. The genome sequence of the obligate intracellular pathogen Lawsonia intracellularis (Deltaproteobacteria), responsible for one of the most economically important diseases in the swine industry worldwide, revealed the presence of a putative ATP/ADP translocase most similar to known ATP/ADP translocases of chlamydiae and rickettsiae (around 47% amino acid sequence identity). The gene coding for the putative ATP/ADP translocase of L. intracellularis (L. intracellularis nucleotide transporter 1 [NTT1(Li)]) was cloned and expressed in the heterologous host Escherichia coli. The transport properties of NTT1(Li) were determined by measuring the uptake of radioactively labeled substrates by E. coli. NTT1(Li) transported ATP in a counterexchange mode with ADP in a highly specific manner; the substrate affinities determined were 236.3 (+/- 36.5) microM for ATP and 275.2 (+/- 28.1) microM for ADP, identifying this protein as a functional ATP/ADP translocase. NTT1(Li) is the first ATP/ADP translocase from a bacterium not related to Chlamydiae or Rickettsiales, showing that energy parasitism by ATP/ADP translocases is more widespread than previously recognized. The occurrence of an ATP/ADP translocase in L. intracellularis is explained by a relatively recent horizontal gene transfer event with rickettsiae as donors. PMID:18606736

Schmitz-Esser, Stephan; Haferkamp, Ilka; Knab, Silvia; Penz, Thomas; Ast, Michelle; Kohl, Christian; Wagner, Michael; Horn, Matthias

2008-09-01

331

Lawsonia intracellularis Contains a Gene Encoding a Functional Rickettsia-Like ATP/ADP Translocase for Host Exploitation?  

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ATP/ADP translocases are a hallmark of obligate intracellular pathogens related to chlamydiae and rickettsiae. These proteins catalyze the highly specific exchange of bacterial ADP against host ATP and thus allow bacteria to exploit their hosts' energy pool, a process also referred to as energy parasitism. The genome sequence of the obligate intracellular pathogen Lawsonia intracellularis (Deltaproteobacteria), responsible for one of the most economically important diseases in the swine industry worldwide, revealed the presence of a putative ATP/ADP translocase most similar to known ATP/ADP translocases of chlamydiae and rickettsiae (around 47% amino acid sequence identity). The gene coding for the putative ATP/ADP translocase of L. intracellularis (L. intracellularis nucleotide transporter 1 [NTT1Li]) was cloned and expressed in the heterologous host Escherichia coli. The transport properties of NTT1Li were determined by measuring the uptake of radioactively labeled substrates by E. coli. NTT1Li transported ATP in a counterexchange mode with ADP in a highly specific manner; the substrate affinities determined were 236.3 (± 36.5) ?M for ATP and 275.2 (± 28.1) ?M for ADP, identifying this protein as a functional ATP/ADP translocase. NTT1Li is the first ATP/ADP translocase from a bacterium not related to Chlamydiae or Rickettsiales, showing that energy parasitism by ATP/ADP translocases is more widespread than previously recognized. The occurrence of an ATP/ADP translocase in L. intracellularis is explained by a relatively recent horizontal gene transfer event with rickettsiae as donors.

Schmitz-Esser, Stephan; Haferkamp, Ilka; Knab, Silvia; Penz, Thomas; Ast, Michelle; Kohl, Christian; Wagner, Michael; Horn, Matthias

2008-01-01

332

por láser  

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Full Text Available En el presente trabajo, la técnica Espectroscopia de plasmas producidos por láser (Laser-induced breakdown spectroscopy –LIBS– se aplicó a la determinación cuan- titativa de Na en agua natural dulce, de interés en agricultura para el estudio de la alcalinidad de aguas de regadío. Para efectuar el análisis, se prepararon soluciones con concentraciones conocidas del analito, se mezclaron con óxido de calcio y se compactaron en pastillas sólidas. Los plasmas se produjeron en aire a presión atmos- férica utilizando un láser pulsado Nd:YAG. Se construyó una curva de calibración y se calculó el límite de detección. Se analizaron muestras de agua natural y los resultados se compararon con los obtenidos mediante espectroscopia de absorción atómica. Se demostró la factibilidad del método para la determinación de Na en agua natural dulce.

Mayra Garcimu\\u00F1o

2013-01-01

333

Tratamiento adyuvante con quimioterapia y radioterapia luego de gastrectomía y disección ganglionar D2 en pacientes con cáncer gástrico en el Instituto Nacional de Enfermedades Neoplásicas, Lima, Perú / Adjuvant chemo radiotherapy after gastrectomy and D2 lymphadenectomy in patients with gastric cancer in the National Institute of Cancer, Lima, Peru  

Scientific Electronic Library Online (English)

Full Text Available SciELO Peru | Language: Spanish Abstract in spanish Introducción: La quimio radioterapia adyuvante es una alternativa de tratamiento especialmente para pacientes con cirugías inferiores a D2. El estudio INT016 estableció la quimio radioterapia adyuvante como el cuidado estándar para adenocarcinoma resecado de alto riesgo del estómago en Estados Unido [...] s, sin embargo, se vio obstaculizado por la cirugía sub óptima. Existe controversia acerca de la eficacia de esta terapia adyuvante en pacientes sometidos a linfandenectomía D2. En nuestra institución la linfandenectomía D2 es la cirugía estándar para cáncer gástrico. Objetivo: Demostrar que la terapia adyuvante de quimio y radioterapia en pacientes con cáncer gástrico sometidos a gastrectomía y disección ganglionar D2 es efectiva. Materiales y métodos: Estudio retrospectivo de pacientes con adenocarcinoma gástrico estadío II a IV M0 quienes se sometieron a resección curativa en el Instituto Nacional de Enfermedades Neoplásicas, Lima-Perú en el periodo 2001 a 2006. El tratamiento estándar en la Institución es la linfandenectomía D2 y quimio radioterapia adyuvante de acuerdo al INT016. Las curvas de sobrevida fueron estimadas con el método Kaplan-Meier y comparadas con la prueba logrank. Resultados: 84 pacientes fueron incluidos 60,7% hombres y 39,3% mujeres. La edad media fue 49,5 años. Los estadios patológicos fueron T1-T2 (15,5%), T3-T4 (84,5%), N0-N1 (10,7%), N2-N3 (89,3%). Linfandenectomía D2 fue desarrollada en todos los pacientes. Encontramos una sobrevida libre de enfermedad a 3 años de 17% y una sobrevida global de 23,9%. Cuando se analiza por subgrupos, la sobrevida global en los grupos N1, N2 y N3 fueron 66,7%, 58,9% y 18,3%, respectivamente y la sobrevida libre de enfermedad fue 100%, 51,9% y 16,3%, respectivamente. Conclusiones: La quimio radioterapia adyuvante podría ser una alternativa para disminuir el riesgo de muerte y recaída a tres años principalmente en pacientes con ganglios positivos N1-N2, quienes se sometieron a resección curativa con linfandenectomía D2, pero la recurrencia fue más frecuente en ganglios positivos N3, la quimioterapia en este grupo de pacientes podría ser una alternativa para disminuir la tasa de recaída. Abstract in english Introduction: Adjuvant chemo radiotherapy is the standard treatment in Western countries in gastric cancer patients submitted to curative resection. INT0116 pivotal trial established adjuvant chemo radiation as the standard care for resected high risk adenocarcinoma of the stomach in US however was [...] hampered by suboptimal surgery. There is controversial data about efficacy of this adjuvant therapy in patients who have undergone D2 lymphadenectomy predominantly. In our hospital D2 lymphadenectomy is standard surgery for gastric cancer. Objective: To prove that chemo and radio therapy post gastrectomy and D2 linphadenectomy in patients’ with gastric cancer is effective. Material and methods: Retrospective study with gastric adenocarcinoma patients stage II to IV M0 who underwent curative resection at INEN (Instituto Nacional de Enfermedades Neoplasicas) Lima-Peru between 2001 and 2006. Standard treatment at institution is D2 lymphadenectomy. Chemo radiotherapy according to INT0116 was given like adjuvant therapy. Survival curves were calculated according to Kaplan-Meier method and compared with log-rank test. Results: 84 patients were included 60.7% male and 39.3% female. Mean age was 49.5 years old. The pathologic stages were T1-T2 (15.5%), T3-T4 (84.5%), N0-N1 (10.7%), N2-N3 (89.3%). D2 lymphadenectomy was performed in all patients. The 3-year DFS was 17% and 3-year overall survival was 23.9%. However when we analyzed by subgroups the overall survival, was in group N1 (66.7%) and in group N2 (58.9%) and N3 (18.3%) and 3 years DFS by subgroups were N1 (100%), N2 (51.9%) and N3 (16.3%). Conclusions: Adjuvant chemo radiotherapy decreased risk of death and relapse to three years mainly in patients with node positive N1-N2, who underwent curative

Paola, Montenegro; Lourdes, Flores; Shirley, Quintana; Luís, Casanova; Eloy, Ruíz; Jorge, León; Raúl, Mantilla.

334

Factores asociados al pronóstico de pacientes operados por cáncer gástrico avanzado Pathological factors associated with survival in advanced gastric cancer  

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Full Text Available Introducción: El estudio de la pieza operatoria de pacientes resecados por cáncer gástrico (CG ha permitido identificar variables anatomo-patológicas con valor pronóstico en la supervivencia (SV y recurrencia de estos pacientes, siendo el compromiso ganglionar linfático y nivel de infiltración tumoral, los factores más relevantes identificados. El objetivo de este estudio es determinar asociación entre variables clínicas y morfológicas con la SV de pacientes resecados por CG avanzado (CGA. Material y Método: Estudio de cohorte retrospectiva. Se estudiaron variables clínicas y morfológicas de 299 pacientes operados por CGA entre enero de 1986-diciembre de 2001. Los datos fueron obtenidos desde la Unidad de Anatomía Patológica del Hospital Hernán Henríquez Aravena de Temuco. Se aplicó estadística descriptiva y analítica; confección de curvas de supervivencia, y finalmente se aplicaron modelos de regresión logística para realizar ajuste, calcular odds ratios y sus respectivos intervalos de confianza de 95%o. Resultados: La cohorte tuvo una mediana de edad de 63 años y el 68% de ella correspondió al género masculino. Con una mediana de seguimiento de 21 meses (1 a 206, se observó una SV actuarial global a 5 y 10 años de 39% y 34% respectivamente. En el análisis bivariado, se verificó asociación con la SV en: etapa tumoral macroscópica, localization y tamaño tumoral, tipo según Bormann, nivel de infiltración, grado de diferenciación histológico, tipo histológico según Lauren, Ming y Nakamura, estado ganglionar linfático (N, estadio TNM y resultado de la resección realizada. Conclusiones: Las variables mencionadas deben ser cuidadosamente evaluadas al momento de decidir terapias en pacientes con CGA.Background: The degree of tumor infiltration and lymph node involvement are the most relevant pathological features to determine prognosis of advanced gastric cancer. Aim: To determine the association between clinical and pathological features of advanced gastric cancer and patient survival. Material and Methods: The pathological records of patients with advanced gastric cancer subjected to gastrectomy and lymph node excision between 1986 and 2007 were analyzed. Follow up was performed according to data in the clinical records and death certificates obtained at the Chilean National Death Registry. The main outcome analyzed was survival after surgery. Results: The records of 299 patients aged 62 ± 11 years (68% males, were analyzed. Mean follow up ranged from 1 to 206 months. Five and 10 years actuarial survival was 39 and 34%, respectively. The pathological predictors of survival were microscopic tumor stage, tumor size and location, Bormann classification, infiltration level, degree of differentiation, pathological type of tumor according to Lauren, Ming y Nakamura, lymph node involvement and the absence of residual tumor after surgical excision. Conclusions: The pathological study of the surgical piece in advanced gastric cancer has important prognostic implications.

ÓSCAR TAPIA E

2011-04-01

335

Transovarial transmission of Rickettsia tsutsugamushi in Leptotrombidium pallidum successively reared in the laboratory.  

Science.gov (United States)

Engorged larvae of Leptotrombidium pallidum were collected from a wild rodent, Apodemus speciosus, and reared in a plastic container fed with fresh eggs of colembolla, Sinella curviseta, under moist conditions at 25 degrees C. Fully developed adults were separated into individual containers and a proven male was paired with a suspected female. Of the pairings, 38 produced larvae and 4 infected colonies were established. Females in these colonies were shown to be infected with Karp type of Rickettsia tsutsugamushi (Rt) by the method of isolating Rt from individual mites using mouse passage. Males were negative for Rt. Vertical transmission of Rt was monitored in each infected colony. The female of parent generation (P) produced F1 and F2 generations. At F1 generation, 11 males and 12 females maintained until production of eggs and all adults were positive for Rt by detection from individual mites. By sibmating of F1 adults, 11 males and 14 females developed at F2 generation and all males and females were proven to be positive for Rt. From 4 pairs of infected males and infected females at F1 generation, 2, 5, 21 and 35 larvae were collected as samples, respectively, and all larvae were positive for Rt by individual detection. Spermatophores from infected males at F2 generation were examined for Rt and none of 29 spermatophores were positive for Rt. From these results, the transmission rate of Rt from P to F1 and from F1 to F2 was 100% through eggs from infected females but not through spermatophores from infected males. PMID:3149693

Takahashi, M; Murata, M; Nogami, S; Hori, E; Kawamura, A; Tanaka, H

1988-10-01

336

Lymphokine-activated-killer-mediated lysis of cells infected with typhus group rickettsiae can be inhibited by OKT3 monoclonal antibody.  

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We examined the ability of monoclonal antibodies directed against lymphocyte surface antigens to block the lysis of typhus group rickettsia-infected cells by lymphokine-activated killer effectors. Lysis was significantly inhibited by OKT3 monoclonal antibody but not by OKT4 or OKT8 monoclonal antibody.

Carl, M.; Dasch, G. A.

1986-01-01

337

The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite.  

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Full Text Available We sequenced the genome of Rickettsia felis, a flea-associated obligate intracellular alpha-proteobacterium causing spotted fever in humans. Besides a circular chromosome of 1,485,148 bp, R. felis exhibits the first putative conjugative plasmid identified among obligate intracellular bacteria. This plasmid is found in a short (39,263 bp and a long (62,829 bp form. R. felis contrasts with previously sequenced Rickettsia in terms of many other features, including a number of transposases, several chromosomal toxin-antitoxin genes, many more spoT genes, and a very large number of ankyrin- and tetratricopeptide-motif-containing genes. Host-invasion-related genes for patatin and RickA were found. Several phenotypes predicted from genome analysis were experimentally tested: conjugative pili and mating were observed, as well as beta-lactamase activity, actin-polymerization-driven mobility, and hemolytic properties. Our study demonstrates that complete genome sequencing is the fastest approach to reveal phenotypic characters of recently cultured obligate intracellular bacteria.

2005-08-01

338

Evidence of exposure to spotted fever group rickettsiae among Arizona dogs outside a previously documented outbreak area.  

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Since 2003, two communities in eastern Arizona have experienced a sustained outbreak of Rocky Mountain spotted fever (RMSF), caused by Rickettsia rickettsii, associated with transmission by Rhipicephalus sanguineus, the brown dog tick; 70 human cases, including eight deaths, were reported from these communities during 2003 through 2008. In both of the affected communities, antibodies to spotted fever group rickettsiae (SFGR) were present in dogs before the notice of the first human cases, suggesting that dogs may serve as useful sentinels for human risk of RMSF in this region. During 2005 and 2006, an exploratory serosurvey was conducted among stray and relinquished dogs presenting to animal control facilities in eastern Arizona located outside the area where human cases had been reported. Antibodies to SFGR were detected in 5.7% (14 of 247) dogs assessed outside the RMSF outbreak area. Animal shelters located in counties that either included or shared large borders with the outbreak area were significantly more likely to have seropositive dogs than facilities in more geographically separated counties (P = 0.01). In addition, stray dogs were significantly more likely to be antibody-positive than relinquished animals (P = 0.01), suggesting that control of stray dog populations should be considered as a means of limiting SFGR transmission in this region. The findings from this study may be extrapolated to suggest that the current risk for human RMSF infection may extend beyond the noted outbreak area. Heightened surveillance for human disease is needed in the region. PMID:20042069

McQuiston, J H; Guerra, M A; Watts, M R; Lawaczeck, E; Levy, C; Nicholson, W L; Adjemian, J; Swerdlow, D L

2011-03-01

339

SNP-based real-time pyrosequencing as a sensitive and specific tool for identification and differentiation of Rickettsia species in Ixodes ricinus ticks  

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Full Text Available Abstract Background Rickettsioses are caused by pathogenic species of the genus Rickettsia and play an important role as emerging diseases. The bacteria are transmitted to mammal hosts including humans by arthropod vectors. Since detection, especially in tick vectors, is usually based on PCR with genus-specific primers to include different occurring Rickettsia species, subsequent species identification is mainly achieved by Sanger sequencing. In the present study a real-time pyrosequencing approach was established with the objective to differentiate between species occurring in German Ixodes ticks, which are R. helvetica, R. monacensis, R. massiliae, and R. felis. Tick material from a quantitative real-time PCR (qPCR based study on Rickettsia-infections in I. ricinus allowed direct comparison of both sequencing techniques, Sanger and real-time pyrosequencing. Methods A sequence stretch of rickettsial citrate synthase (gltA gene was identified to contain divergent single nucleotide polymorphism (SNP sites suitable for Rickettsia species differentiation. Positive control plasmids inserting the respective target sequence of each Rickettsia species of interest were constructed for initial establishment of the real-time pyrosequencing approach using Qiagen’s PSQ 96MA Pyrosequencing System operating in a 96-well format. The approach included an initial amplification reaction followed by the actual pyrosequencing, which is traceable by pyrograms in real-time. Afterwards, real-time pyrosequencing was applied to 263 Ixodes tick samples already detected Rickettsia-positive in previous qPCR experiments. Results Establishment of real-time pyrosequencing using positive control plasmids resulted in accurate detection of all SNPs in all included Rickettsia species. The method was then applied to 263 Rickettsia-positive Ixodes ricinus samples, of which 153 (58.2% could be identified for their species (151 R. helvetica and 2 R. monacensis by previous custom Sanger sequencing. Real-time pyrosequencing identified all Sanger-determined ticks as well as 35 previously undifferentiated ticks resulting in a total number of 188 (71.5% identified samples. Pyrosequencing sensitivity was found to be strongly dependent on gltA copy numbers in the reaction setup. Whereas less than 101 copies in the initial amplification reaction resulted in identification of 15.1% of the samples only, the percentage increased to 54.2% at 101-102 copies, to 95.6% at >102-103 copies and reached 100% samples identified for their Rickettsia species if more than 103 copies were present in the template. Conclusions The established real-time pyrosequencing approach represents a reliable method for detection and differentiation of Rickettsia spp. present in I. ricinus diagnostic material and prevalence studies. Furthermore, the method proved to be faster, more cost-effective as well as more sensitive than custom Sanger sequencing with simultaneous high specificity.

Janecek Elisabeth

2012-10-01

340

Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA.  

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Currently, the genotypic identification of the spotted fever group (SFG) rickettsiae is based on restriction fragment length polymorphism analysis of PCR-amplified genes coding for the enzyme citrate synthase and the surface proteins rOmpA and rOmpB. A set of useful restriction endonucleases was found following comparison of Rickettsia rickettsii and R. prowazekii sequences. However, by using three PCR amplifications and four enzyme digestions with this set, it was impossible to differentiate...

Roux, V.; Fournier, P. E.; Raoult, D.

1996-01-01

 
 
 
 
341

Avaliação do protocolo PCR4 de Marchetti em tecidos parafinizados para o diagnóstico da tuberculose cutânea e ganglionar / Evaluation of Marchetti PCR4 amplification assay to the diagnosis of cutaneous and lymph node tuberculosis from formalin-fixed paraffin-embedded tissue  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese INTRODUÇÃO: A tuberculose cutaneoganglionar (TbCG) corresponde a 25,4% dos casos de tuberculose (Tb) extrapulmonar no estado do Amazonas. Os métodos tradicionais, bacteriológicos e histopatológicos envolvem algumas dificuldades diagnósticas, e a reação em cadeia da polimerase (PCR) surge como método [...] alternativo, podendo propiciar resultados específicos e em menor tempo. Nesse sentido, verificou-se a acurácia do protocolo PCR4 de Marchetti et al. no diagnóstico da TbCG comparativamente aos métodos bacteriológicos e histopatológicos. MATERIAIS E MÉTODOS: Realizou-se o nested-PCR com oligonucleotídeos para a IS6110 do complexo do M. tuberculosis em 83 amostras parafinizadas, sendo 52 cutâneas e 31 ganglionares, de pacientes clinicamente suspeitos de TbCG. Todos os casos foram avaliados pelos métodos bacteriológicos e histopatológicos. Foi realizada análise da acurácia entre os resultados obtidos na PCR em relação ao cultivo e à histopatologia. RESULTADOS E DISCUSSÃO: A positividade da PCR em todos os casos estudados foi de 50,6% (42/83), sendo de 59,6% (31/52) em amostras cutâneas e de 35,5% (11/31) nas ganglionares. Em ambos os grupos foram observados resultados falso-positivos e falso-negativos. Algumas hipóteses que podem justificar estes resultados estão relacionadas à presença da IS6110 em micobactérias ambientais da região amazônica e à não-padronização da amostra de DNA amplificado. CONCLUSÃO: O protocolo em avaliação apresentou positividade em percentual semelhante a diferentes protocolos existentes na literatura. Sugere-se o uso da PCR em tecidos parafinizados associada com o cultivo ou com a histopatologia para o diagnóstico definitivo de Tb ganglionar. Para as lesões cutâneas continua sendo necessária a busca de protocolo que amplie a acurácia do método. Abstract in english BACKGROUND: Cutaneous lymph node tuberculosis (CLTb) represents 25.4% of all cases of extra-pulmonary Tb in the state of Amazonas. The current methods of diagnose including bacteriological and histopathological assays involve some technical difficulties, and the polymerase chain reaction - PCR arise [...] as an alternative method allowing specific results faster than the others. In this context the accuracy of PCR4 Marchetti et al. protocol was compared with traditional methods. MATERIAL AND METHOD: Nested-PCR for IS6110 (123 pb) were applied on 83 CLTb suspicious formalin fixed and paraffin embedded samples of tissues (52 cutaneous and 31 lymph node), obtained from 1997 to 2002. All cases were evaluated by bacteriological and histopathological methods. Accuracy analyses were carried out between the PCR amplification results and those related on bacteriological and histopathological methods. RESULTS AND DISCUSSIONS: Positive results of PCR4 were about 50.6% (59.6% in cutaneous samples and of 35.5% in lymph nodes samples). In both groups were observed false-negative and false-positive results. Some hypotheses that explain those results are related to the presence of IS6110 in environmental mycobacterias in the Amazon region and the absence of standardized DNA concentration to amplification assays. CONCLUSIONS: The proposed protocol was as positive as others ones available in the literature. Definitive Tb diagnostic can be obtained on lymph node paraffin embedded PCR in association with bacteriological or histopathological method. A better accuracy of an amplification assay applied to cutaneous Tb suspicious lesions has to be still under research.

Luciana Botinelly Mendonça, Fujimoto; Júlia Ignez, Salem; Maurício Morishi, Ogusku; Luiz Carlos de Lima, Ferreira.

342

Avaliação do protocolo PCR4 de Marchetti em tecidos parafinizados para o diagnóstico da tuberculose cutânea e ganglionar Evaluation of Marchetti PCR4 amplification assay to the diagnosis of cutaneous and lymph node tuberculosis from formalin-fixed paraffin-embedded tissue  

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Full Text Available INTRODUÇÃO: A tuberculose cutaneoganglionar (TbCG corresponde a 25,4% dos casos de tuberculose (Tb extrapulmonar no estado do Amazonas. Os métodos tradicionais, bacteriológicos e histopatológicos envolvem algumas dificuldades diagnósticas, e a reação em cadeia da polimerase (PCR surge como método alternativo, podendo propiciar resultados específicos e em menor tempo. Nesse sentido, verificou-se a acurácia do protocolo PCR4 de Marchetti et al. no diagnóstico da TbCG comparativamente aos métodos bacteriológicos e histopatológicos. MATERIAIS E MÉTODOS: Realizou-se o nested-PCR com oligonucleotídeos para a IS6110 do complexo do M. tuberculosis em 83 amostras parafinizadas, sendo 52 cutâneas e 31 ganglionares, de pacientes clinicamente suspeitos de TbCG. Todos os casos foram avaliados pelos métodos bacteriológicos e histopatológicos. Foi realizada análise da acurácia entre os resultados obtidos na PCR em relação ao cultivo e à histopatologia. RESULTADOS E DISCUSSÃO: A positividade da PCR em todos os casos estudados foi de 50,6% (42/83, sendo de 59,6% (31/52 em amostras cutâneas e de 35,5% (11/31 nas ganglionares. Em ambos os grupos foram observados resultados falso-positivos e falso-negativos. Algumas hipóteses que podem justificar estes resultados estão relacionadas à presença da IS6110 em micobactérias ambientais da região amazônica e à não-padronização da amostra de DNA amplificado. CONCLUSÃO: O protocolo em avaliação apresentou positividade em percentual semelhante a diferentes protocolos existentes na literatura. Sugere-se o uso da PCR em tecidos parafinizados associada com o cultivo ou com a histopatologia para o diagnóstico definitivo de Tb ganglionar. Para as lesões cutâneas continua sendo necessária a busca de protocolo que amplie a acurácia do método.BACKGROUND: Cutaneous lymph node tuberculosis (CLTb represents 25.4% of all cases of extra-pulmonary Tb in the state of Amazonas. The current methods of diagnose including bacteriological and histopathological assays involve some technical difficulties, and the polymerase chain reaction - PCR arise as an alternative method allowing specific results faster than the others. In this context the accuracy of PCR4 Marchetti et al. protocol was compared with traditional methods. MATERIAL AND METHOD: Nested-PCR for IS6110 (123 pb were applied on 83 CLTb suspicious formalin fixed and paraffin embedded samples of tissues (52 cutaneous and 31 lymph node, obtained from 1997 to 2002. All cases were evaluated by bacteriological and histopathological methods. Accuracy analyses were carried out between the PCR amplification results and those related on bacteriological and histopathological methods. RESULTS AND DISCUSSIONS: Positive results of PCR4 were about 50.6% (59.6% in cutaneous samples and of 35.5% in lymph nodes samples. In both groups were observed false-negative and false-positive results. Some hypotheses that explain those results are related to the presence of IS6110 in environmental mycobacterias in the Amazon region and the absence of standardized DNA concentration to amplification assays. CONCLUSIONS: The proposed protocol was as positive as others ones available in the literature. Definitive Tb diagnostic can be obtained on lymph node paraffin embedded PCR in association with bacteriological or histopathological method. A better accuracy of an amplification assay applied to cutaneous Tb suspicious lesions has to be still under research.

Luciana Botinelly Mendonça Fujimoto

2007-06-01

343

Characterization of rickettsia rickettsii in a case of Fatal Brazilian spotted fever in the city of Rio de Janeiro, Brazil  

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Full Text Available SciELO Brazil | Language: English Abstract in english A lethal case of Brazilian spotted fever (BSF) is presented. Clinical features were initially of gastrointestinal involvement and evolved with progression to septic shock, meningoencephalitis and death on the 6th day of illness. Indirect immunofluorescence assay (IFA) for spotted fever group rickett [...] sia (SFGR) was non-reactive. Diagnosis was confirmed by the polymerase chain reaction (PCR) and the nucleotide sequencing of a fragment of the ompA gene showed 100% homology to Rickettsia rickettsii. BSF has not been reported in the city of Rio de Janeiro in the last three decades, and the present description should alert the clinicians to its presence in urban Rio de Janeiro, and to the differential diagnosis with dengue fever, gastroenteritis, leptospirosis and bacterial septic shock, among others.

Lamas, Cristiane; Favacho, Alexsandra; Rozental, Tatiana; Bóia, Márcio N.; Kirsten, Andrei H.; Guterres, Alexandro; Barreira, Jairo; Lemos, Elba Regina S. de.

344

Rickettsiae-infected Ticks in an Endemic Area of Spotted Fever in the State of Minas Gerais, Brazil  

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Full Text Available SciELO Brazil | Language: English Abstract in english A study on tick-borne rickettsiosis was developed in the county of Santa Cruz do Escalvado, State of Minas Gerais, Brazil, where a clinical case of the disease, confirmed by necropsy, had been reported. Of the 1,254 ticks collected, 1,061 belonged to the Amblyomma genus, 57 to the Rhipicephalus sang [...] uineus species, 81 to Boophilus microplus, and 46 to Anocentor nitens. The hemolymph test associated with Giménez staining showed that 18 of the 221 A. cajennense specimens, 1 of the 16 R. sanguineus, 1 of the 22 B. microplus, 3 of the A. nitens, and 1 of the A. ovale contained rickettsia-like microorganisms. Only 3 A. cajennense ticks were positive under direct immunofluorescence. A. cajennense was the only species found on humans

Elba Regina Sampaio de, Lemos; Raimundo Diogo, Machado; Fernando Dias de Ávila, Pires; Sérgio Lisboa, Machado; Lígia Maria Cantarino da, Costa; José Rodrigues, Coura.

345

Comparison of the effectiveness of five different antibiotic regimens on infection with Rickettsia typhi: therapeutic data from 87 cases.  

Science.gov (United States)

This study estimated the clinical effectiveness of five different antibiotic regimens (doxycycline, chloramphenicol, ciprofloxacin, doxycycline plus chloramphenicol, and doxycycline plus ciprofloxacin) administered for infection with Rickettsia typhi in terms of the duration of the fever. Eighty-seven patients with endemic typhus were hospitalized between 1993 and 1998 at the General Hospital of Chania in Chania, Crete, Greece. The mean time to defervescence was 2.9 days for doxycycline, 4.0 days for chloramphenicol, and 4.2 days for ciprofloxacin. In patients receiving combinations of doxycycline plus chloramphenicol and doxycycline plus ciprofloxacin, fever subsided in 3.4 and 4.0 days, respectively. The outcome was favorable in all patients, and no deaths or relapses were observed within two months. PMID:15155995

Gikas, Achilleas; Doukakis, Stephanos; Pediaditis, John; Kastanakis, Serafim; Manios, Andreas; Tselentis, Yiannis

2004-05-01

346

Ulcera lingual como signo único de infección recurrente por micobacteria en un paciente con VIH/SIDA / Lingual ulcer as the only sign of recurrent mycobacterial infection in an HIV/AIDS-infected patient  

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Full Text Available SciELO Spain | Language: Spanish Abstract in spanish Se describe un paciente con VIH/SIDA en el que se identificó una infección por micobacteria en la mucosa bucal, probablemente tuberculosis, en un centro de referencia para VIH/SIDA de la Ciudad de México. El propósito del presente informe es describir los hallazgos clínicos e histológicos en un paci [...] ente con VIH/SIDA, quien después de haber sido tratado exitosamente para tuberculosis ganglionar 4 años antes, presentó una úlcera lingual como único signo que sugirió recurrencia de infección por micobacteria, probablemente tuberculosis. Hombre de 39 años de edad, atendido desde 1991 en el Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", por el diagnóstico de infección con VIH. En 1999, el paciente presentó tuberculosis ganglionar, recibiendo tratamiento antifímico con involución de las adenopatías y desaparición de los síntomas sistémicos. En mayo del 2003 acudió a consulta por presentar una úlcera superficial en lengua, dolorosa, de 4 meses de evolución, de 0.7 cm. de diámetro, bien circunscrita, crateriforme, con bordes ligeramente elevados, irregulares e indurados. El estudio histopatológico mostró inflamación granulomatosa crónica con células gigantes multinucleadas sugestivas de infección por micobacteria, lo cual hizo pensar en recurrencia de tuberculosis, por lo que se indicó rifampicina, pirazinamida, etambutol y estreptomicina. En junio del 2003 el paciente inició TARAA, que incluyó dos ITRAN y un ITRNN. La lesión lingual evolucionó favorablemente, con cicatrización parcial a la primera semana y remisión total a los 45 días del inicio del tratamiento antifímico; a los 7 meses de seguimiento permanece sin lesión. El presente caso tiene la particularidad de que la úlcera lingual fue la única manifestación de infección por micobacteria, sugestiva de tuberculosis, en un paciente con VIH/SIDA, que pudo ocurrir como resultado de la recurrencia del episodio previo de TB ganglionar. Abstract in english The report describes an HIV/AIDS patient seen at a referral center in Mexico City, in whom a mycobacterial infection in the oral mucosa, probably tuberculosis (TB) was identified. The purpose is to describe the clinical and histological findings in an HIV-infected patient, who after being treated su [...] ccessfully for tuberculous lymphangitis 4 years ago, presented with a lingual ulcer as the only suggestive sign of recurrence of mycobacterial infection, probably M. tuberculosis. A 39-year-old man seen inthe HIV clinic of the Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" in Mexico City since 1991 for HIV infection. In 1999 the patient developed tuberculous lymphangitis; he was managed with a 4-drug regimen for 12 months, with improvement of local and systemic symptoms. In May of 2003, the patient presented a painful superficial lingual ulcer, 0.7 cm in diameter, well circumscribed, crateriform with slightly elevated, irregular and indurated borders, of 4 months duration. The histopathological examination showed chronic granulomatous inflammation with giant multinucleated cells, suggestive of mycobacterial infection, and recurrence of TB was considered. Rifampin, isoniazide, pyrazinamide, ethambutol and streptomycin were administered. The lingual lesion improved with partial healing at the first week and total remission at 45 days after the beginning of the antituberculous treatment. In June, 2003, the patient began highly active antiretroviral therapy (HAART) that included two NRTIs and one NNRTI. At 7 months of follow-up, the patient remains free of lingual lesions. The particularity of the present case is that the lingual ulcer was the only sign of infection by mycobacteria, suggestive of TB, in an HIV/AIDS patient that probably represented a recurrence of a previous episode.

Velia, Ramírez Amador; Gabriela, Anaya Saavedra; Imelda, González Ramírez; Juan Luis, Mosqueda Gómez; Lilly, Esquivel Pedraza; Edgardo, Reyes Gutiérrez; Juan, Sierra Madero.

347

Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands  

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Full Text Available Abstract Background Awareness for flea- and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The role of Ixodes ricinus ticks in the natural cycle of Bartonella spp. and the transmission of these bacteria to humans is unclear. Rickettsia spp. have also been reported from as well ticks as also from fleas. However, to date no flea-borne Rickettsia spp. were reported from the Netherlands. Here, the presence of Bartonellaceae and Rickettsiae in ectoparasites was investigated using molecular detection and identification on part of the gltA- and 16S rRNA-genes. Results The zoonotic Bartonella clarridgeiae and Rickettsia felis were detected for the first time in Dutch cat fleas. B. henselae was found in cat fleas and B. schoenbuchensis in ticks and keds feeding on deer. Two Bartonella species, previously identified in rodents, were found in wild mice and their fleas. However, none of these microorganisms were found in 1719 questing Ixodes ricinus ticks. Notably, the gltA gene amplified from DNA lysates of approximately 10% of the questing nymph and adult ticks was similar to that of an uncultured Bartonella-related species found in other hard tick species. The gltA gene of this Bartonella-related species was also detected in questing larvae for which a 16S rRNA gene PCR also tested positive for "Candidatus Midichloria mitochondrii". The gltA-gene of the Bartonella-related species found in I. ricinus may therefore be from this endosymbiont. Conclusions We conclude that the risk of acquiring Cat Scratch Disease or a related bartonellosis from questing ticks in the Netherlands is negligible. On the other hand fleas and deer keds are probable vectors for associated Bartonella species between animals and might also transmit Bartonella spp. to humans.

Reimerink Johan R

2011-04-01

348

Transcriptional regulation of the gltA and tlc genes in Rickettsia prowazekii growing in a respiration-deficient host cell  

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The regulation of the citrate synthase (gltA) and ATP/ADP translocase (tlc) genes of the obligate intracellular bacterium, Rickettsia prowazekii, was analyzed in rickettsia-infected respiration-deficient G14 cells. The level of the gltA mRNAII and the tlc mRNA was much lower in the total RNA isolated from the infected G 14 cells grown in 1 g/1 glucose (low glucose, GL) medium than in that from infected G 14 cells grown in 4.5 g/l glucose (high glucose, GH) medium. However, the level of the gltA mRNAI relative to 16 S rRNA was the same in GL and GH media. An increase in the level of the gltA mRNAII and the tlc mRNA could be observed as early as 2 hrs after shifting from GL to GH medium. We conclude that, under these experimental conditions, the tlc promoter and the gltA promoter P2, but not gltA promoter P1, were transcriptionally regulated. Key words: Rickettsia prowazekii; gltA gene; tlC gene; transcriptional regulation; G 14 cells (authors)

1997-10-01

349

Host blood meal-dependent growth ensures transovarial transmission and transstadial passage of Rickettsia sp. phylotype G021 in the western black-legged tick (Ixodes pacificus).  

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In this study, we explored the growth dynamics of Rickettsia sp. phylotype G021 during transovarial transmission and transstadial passage by Ixodes pacificus using real-time quantitative PCR. Four parental engorged I. pacificus females were allowed to complete their developmental stages until the F2-generation eggs yielded unfed larvae. All eggs, larvae, nymphs, and adults tested through 2 generations were found to be infected with phylotype G021. Hence, we conclude that the efficiency of transovarial transmission and transstadial passage of this phylotype in I. pacificus was 100%. Acquisition of a blood meal by all 3 parasitic stages (larva, nymph, adult) significantly increased the rickettsial burden as fed larvae, nymphs, and adults had respective 19-, 12-, and 313-fold increases of rickettsiae compared with unfed ticks representing each developmental stage. I. pacificus eggs contained high rickettsial burdens at the time of oviposition. While I. pacificus egg cells underwent rapid proliferation during early embryonic development, the rickettsiae remained relatively quiescent, which resulted in depressed numbers of phylotype G021 per tick cell. However, the rickettsial burden remained constant over a period of 56 days, as the rate of I. pacificus cell division slowed during later embryonic development. PMID:23876278

Cheng, Du; Lane, Robert S; Moore, Benjamin D; Zhong, Jianmin

2013-09-01

350

[Characterization of Rickettsia spp. circulating in a silent peri-urban focus for Brazilian spotted fever in Caratinga, Minas Gerais, Brazil].  

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The present study was intended to characterize Rickettsia spp. circulating in arthropod vectors in Caratinga, Minas Gerais, Brazil, by PCR and to investigate the presence of antibodies against the spotted fever Rickettsiae group (SFRG) in dogs and horses. 2,610 arthropods were collected and taxonomically identified. DNA samples obtained from these vectors were submitted to PCR and cycle-sequenced. Ctenocephalides and Amblyomma cajennense showed sequences presenting 100.0% homology with R. felis. A sequence obtained from Rhipicephalus sanguineus showed 99.0% homology with R. felis, and a sequence from A. cajennense showed 97.0% homology with R. honei and R. rickettsii. Canine (73) and equine (18) serum samples were tested by indirect fluorescent antibody (IFA) using R. rickettsii antigen. Only three of the equine sera tested (17.0%) had positive antibody titers. Molecular detection of rickettsiae species potentially pathogenic to humans in arthropod vectors and the presence of seroreactivity to SFRG in horses show the risk of transmission of rickettsiosis in this area and the need to maintain continuous epidemiological surveillance for rickettsial diseases. PMID:16583093

Cardoso, Luciane Daniele; Freitas, Renata Nascimento; Mafra, Cláudio Lísias; Neves, Cristiane Vilas Boas; Figueira, Fátima Cristina Bacellar; Labruna, Marcelo Bahia; Gennari, Solange M; Walker, David Hughes; Galvão, Márcio Antônio Moreira

2006-03-01

351

Linfadenitis de causa tuberculosa: diagnóstico por punción aspirativa con aguja fina / Lymphadenitis of tuberculosis etiology: diagnosis by fine needle aspiration biopsy  

Scientific Electronic Library Online (English)

Full Text Available SciELO Cuba | Language: Spanish Abstract in spanish Fundamento: la tuberculosis es un problema serio de salud mundial. La propagación de la infección por el VIH agudiza su impacto. La tuberculosis extrapulmonar supone el 10-20 % del total que padecen los enfermos inmunocompetentes. Caso clínico: se presenta un paciente de treinta y dos años de edad, [...] sexo masculino, raza mestiza, procedente del área rural, con antecedentes de ser paciente con VIH/SIDA, que es ingresado en los servicios de atención clínica del hospital Universitario Manuel Ascunce Domenech, por presentar adenopatías latero- cervical derecha e inguinales, de dos meses de evolución, fiebre inicial de 39?C, tos escasa, sin expectoración. Para la confirmación del diagnóstico clínico inicial de posible tuberculosis ganglionar, se indica entre los complementarios, la Punción Aspirativa con Aguja Fina y se realiza cultivo microbiológico de la muestra obtenida. Conclusiones: el presente tema adquiere importancia en la actualidad, en la que el diagnóstico rápido de la enfermedad tuberculosa es una necesidad, evita tratamientos innecesarios, posibilita el cultivo y permite seleccionar qué pacientes requieren biopsias ganglionares y estudio histopatológico para diagnóstico diferencial y definitivo de la enfermedad. Así como constituye una herramienta más de confirmación del diagnóstico clínico de sospecha, a menos costo. Abstract in english Background: tuberculosis (TB) is a serious worldwide health problem. The spread of HIV infection increases its impact. Extrapulmonary tuberculosis accounts for 10-20 % of TB which suffers inmmunocompetent patients. Clinical case: a 32 years old, male patient, Afro-American descendent, coming from a [...] rural area with a history of HIV/AIDS patient is presented. The patient was admitted in the internal medicine service at the Manuel Ascunce Domenech University Hospital, presenting right laterocervical and inguinal adenopathies of two month of evolution, fever of 39oC, intermittent cough without expectoration. To confirm the initial clinical diagnosis of possible ganglial tuberculosis, fine-needle aspiration biopsy was recommended as a complementary examination and microbiological culture of the sample was performed. Conclusions: this patient´s case has developed importance today, in which quick diagnosis of tuberculosis is a necessity, avoiding unnecessary treatments, enables microbiological culture and allows selecting which patients require ganglion biopsies and histopathological study for differential and definitive diagnosis of the disease. As well as, it is one tool for confirmation of clinical diagnosis of suspicion, at lower cost.

Odalys, Durruthy Wilson; Nayvis, Suazo Valle; Daysi, Perón Ortiz.

352

Factores morfológicos asociados al pronóstico de pacientes operados por cáncer gástrico incipiente / Pathological features associated with survival in early gastric cancer  

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Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Introducción: El cáncer gástrico incipiente (CGI) es aquel que compromete la mucosa o submucosa gástrica independientemente del compromiso ganglionar linfático, estimándose su prevalencia en Chile inferior al 20%o. El objetivo de este estudio es determinar prevalencia de CGI y asociación de variable [...] s biode-mográficas y morfológicas con la supervivencia (SV) de pacientes resecados por CGI. Material y Método: Estudio de cohorte retrospectiva. Se estudiaron variables biodemográficas y morfológicas de 106 pacientes resecados por CGI entre 1986-2007. Se aplicó estadística descriptiva y analítica; confección de curvas de SV, y finalmente se aplicaron modelos de regresión logística para realizar ajuste, calcular odds ratio y sus respectivos intervalos de confianza de 95%. Resultados: 15% correspondió a CGI. La mediana de edad fue 63 años y el 60% correspondió a género masculino con una SV global a 5 años de 91%. Se observaron diferencias estadísticas significativas entre tumores mucosos y submucosos en cuanto a la localización tumoral y compromiso linfonodal junto con presentarse el compromiso nodal más frecuentemente en tumores > 35mm poco diferenciados y difusos de Lauren. El análisis multivariado identificó como factores asociados a la SV: tamaño tumoral, grado de diferenciación histológica en su variedad poco diferenciado, tipo difuso de Lauren y compromiso ganglionar linfático. Conclusiones: Se verificó una prevalencia de CGI de 15%, los que resecados presentan SV de 91% a 5 años. El compromiso linfonodal es un factor asociado a la SV; y además, se relaciona con tamaño tumoral, tipo histológico según Lauren, grado de diferenciación histológico y nivel de infiltración. Abstract in english Background: Early gastric cancer corresponds to those tumors that only involve mucosa and submuco-sa. It is associated with a high survival rate. Aim: To determine pathological factors associated with survival in early gastric cancer. Material and Methods: Analysis of pathological records of 106 pat [...] ients, with a median age of 63 years (60%> males), subjected to a gastrectomy for early gastric cancer. Follow up was performed according to data in the clinical records and death certificates obtained at the Chilean National Death Registry. Results: Five years global survival of patients was 91%o. Lymph node involvement was more common among tumors bigger than 35 mm, with a low degree of differentiation and among those tumors classified as diffuse according to Lauren. Survival was significantly lower for bigger tumors, those with of a low degree of differentiation, diffuse tumors according to Lauren and those with lymph node involvement. Conclusions: Early gastric cancer has a high five years survival. Bigger tumors, those with a low degree of differentiation and those with lymph node involvement are associated with lower survival rates.

ÓSCAR, TAPIA E; PATRICIA, GARCÍA M; CARLOS, MANTEROLA D; MIGUEL, VILLASECA H; JUAN CARLOS, ARAYA O; JUAN CARLOS, ROA S.

353

Factores morfológicos asociados al pronóstico de pacientes operados por cáncer gástrico incipiente Pathological features associated with survival in early gastric cancer  

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Full Text Available Introducción: El cáncer gástrico incipiente (CGI es aquel que compromete la mucosa o submucosa gástrica independientemente del compromiso ganglionar linfático, estimándose su prevalencia en Chile inferior al 20%o. El objetivo de este estudio es determinar prevalencia de CGI y asociación de variables biode-mográficas y morfológicas con la supervivencia (SV de pacientes resecados por CGI. Material y Método: Estudio de cohorte retrospectiva. Se estudiaron variables biodemográficas y morfológicas de 106 pacientes resecados por CGI entre 1986-2007. Se aplicó estadística descriptiva y analítica; confección de curvas de SV, y finalmente se aplicaron modelos de regresión logística para realizar ajuste, calcular odds ratio y sus respectivos intervalos de confianza de 95%. Resultados: 15% correspondió a CGI. La mediana de edad fue 63 años y el 60% correspondió a género masculino con una SV global a 5 años de 91%. Se observaron diferencias estadísticas significativas entre tumores mucosos y submucosos en cuanto a la localización tumoral y compromiso linfonodal junto con presentarse el compromiso nodal más frecuentemente en tumores > 35mm poco diferenciados y difusos de Lauren. El análisis multivariado identificó como factores asociados a la SV: tamaño tumoral, grado de diferenciación histológica en su variedad poco diferenciado, tipo difuso de Lauren y compromiso ganglionar linfático. Conclusiones: Se verificó una prevalencia de CGI de 15%, los que resecados presentan SV de 91% a 5 años. El compromiso linfonodal es un factor asociado a la SV; y además, se relaciona con tamaño tumoral, tipo histológico según Lauren, grado de diferenciación histológico y nivel de infiltración.Background: Early gastric cancer corresponds to those tumors that only involve mucosa and submuco-sa. It is associated with a high survival rate. Aim: To determine pathological factors associated with survival in early gastric cancer. Material and Methods: Analysis of pathological records of 106 patients, with a median age of 63 years (60%> males, subjected to a gastrectomy for early gastric cancer. Follow up was performed according to data in the clinical records and death certificates obtained at the Chilean National Death Registry. Results: Five years global survival of patients was 91%o. Lymph node involvement was more common among tumors bigger than 35 mm, with a low degree of differentiation and among those tumors classified as diffuse according to Lauren. Survival was significantly lower for bigger tumors, those with of a low degree of differentiation, diffuse tumors according to Lauren and those with lymph node involvement. Conclusions: Early gastric cancer has a high five years survival. Bigger tumors, those with a low degree of differentiation and those with lymph node involvement are associated with lower survival rates.

ÓSCAR TAPIA E

2011-04-01

354

Endoftalmite por Candida albicans  

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Full Text Available O autor descreve os aspectos epidemiológicos, histopatológicos e clínicos da endoftalmite endógena por Candida albicans. Apresenta ainda novos métodos diagnósticos e opções terapêuticas utilizadas no tratamento das infecções fúngicas intra-oculares, por meio de revisão bibliográfica.

Serracarbassa Pedro Duraes

2003-01-01

355

Anticorpos anti-rickettsias do grupo da febre maculosa em equídeos e caninos no norte do Estado do Paraná, Brasil / Anti rickettsia-antibody for spotted fever group in horses and dogs in the North of Paraná Stated, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in english The Brazilian Spotted Fever (BSF) is a zoonosis that can be fatal if not trteated. As there are few studies of the BSF in the Paraná State, the occurrence of BSF was serologically investigated in dogs and horses by indirect immunofluorescence assay (IFA), in the North of that State, in animals that [...] are regarded as sentinels for BSF. A total of 241 samples of sera of equine, four of asinine, and 29 of dogs were collected in nine farms of three municipalities in the North of Paraná: Arapongas, Douradina, and Umuarama. From fifteen samples of equine serum with positive titres for RIFI, eight (53.3 %) had titre of 64 against R. rickettsii, two (13.3 %) 128 against R. rickettsii, and five (33.3 %) were reactants for R. parkeri and R. rickettsii, with titres ranging from 64 to 2048, and 128 to 1024, respectively. The results showed that domestic animals, sentinels for BSF, are under low exposition to ticks infected with spotted fever group Rickettsia, indicating low risk of human infection by these agents in the studied area.

Otomura, F.H.; Sangioni, L.A.; Pacheco, R.C.; Labruna, M.B.; Galhardo, J.A.; Ribeiro, M.G.; Teodoro, U..

356

Síndrome de Frey por submaxilectomía y tratamiento con toxina botulínica / Frey syndrome secondary to submaxillectomy and botulinic treatment  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: Spanish Abstract in spanish El síndrome de Frey (SF) se caracteriza por rubor, calor y sudación en la región maseterina y geniana durante las comidas. El SF se observa en el 20-60% de los pacientes sometidos a parotidectomía. Presentamos un caso con SF secundario a la ablación de la glándula submaxilar en un varón de 30 años d [...] e edad, sin antecedentes patológicos, que consultó en el año 2000 por adenopatía cervical izquierda. La misma fue biopsiada y la anatomía patológica informó carcinoma medular de tiroides. El paciente presentaba múltiples metástasis hepáticas y pulmonares, motivo por el cual recibió tratamiento quimioterápico con cisplatino plus doxorrubicina (seis ciclos) con respuesta completa, finalizando dicho esquema en marzo del 2002. En julio del 2002 se realizó tiroidectomía total más vaciamiento ganglionar izquierdo con resección de la glándula submaxilar. Durante la cirugía se lesionó la rama mentoniana del nervio cervicofacial, rama del VII par craneal. En septiembre del 2004 consultó por episodios de sudación durante las comidas en la región submaxilar izquierda. Se realizó prueba de Minor y luego se procedió a aplicar toxina botulínica tipo A en la región afectada, a razón de 2.5 UI por punto (cm²), a un total de 17 puntos. El control a los 21 días demostró más de un 95% de efectividad en la resolución del SF. El efecto duró un año, y una segunda reinfiltración mostró similar respuesta. No hallamos descripciones anteriores de SF en región submaxilar; se presenta su tratamiento satisfactorio con toxina botulínica tipo A. Abstract in english A case of Frey syndrome (FS) secondary to submaxillar gland exeresis is presented and the results of the treatment with botulinum toxin (BTX) type A. FS is a condition of sweating cheek and preauricular area during mealtime as a sequel detected in about 20-60% of patients after parotidectomy. The cl [...] inical symptoms include swelling, flushing and hyperhidrosis. The treatment choice for this condition is intracutaneous injection of BTX type A which blocks acetylcholine release at the sweat glands. A 30-year-old man, with thyroid medullar carcinoma diagnosed in 2002 received 6 cicles of cisplatin plus doxorubicin previous to the thyroidectomy with anterolateral neck dissection. During surgery the left ramus marginalis mandibulae was damaged. Two years later the patient referred sweating in submaxillar region during meals. CT scan demonstrated the absence of left submaxillar gland. Minor's test disclosed the affected area and BTX type A was injected (2.5 U/cm²/17 points). A twenty-one-day control showed a 95% reduction of the affected skin area. Persistent efficacy was observed up to one year followup time when he was reinjected. The FS, also known as "gustatory hyperhidrosis", was probably first reported by M. Duphenix in 1757. Lucja Frey considered its physiopathology as a disorder of both sympathetic and parasympathetic innervation. In our case the FS was caused by a misdirected regeneration of postganglionic parasympathetic nerve fibers that arrised from the nervus lingualis rami ganglionares of the nervus trigeminus. After nerve injury the colinergic parasympathetic fibers seek out colinergic receptors -sympathetic receptors of the skin- innervating sweat glands and small skin vessels. All previous cases were located at masseter region post-parotidectomy. We have not found any description of FS in the submaxillary region. The self-assessed efficacy of the treatment with a hyperhidrosis disease severity scale revealed a very satisfied patient at 20 months follow-up after being injected twice with BTX type A.

Baéz, Alejandra; Paleari, Julieta; Durán, María Nöel; Rudy, Tamara; Califano, Inés; Barbosa, Nicolás; Casas Parera, Ignacio.

357

Síndrome de Frey por submaxilectomía y tratamiento con toxina botulínica Frey syndrome secondary to submaxillectomy and botulinic treatment  

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Full Text Available El síndrome de Frey (SF se caracteriza por rubor, calor y sudación en la región maseterina y geniana durante las comidas. El SF se observa en el 20-60% de los pacientes sometidos a parotidectomía. Presentamos un caso con SF secundario a la ablación de la glándula submaxilar en un varón de 30 años de edad, sin antecedentes patológicos, que consultó en el año 2000 por adenopatía cervical izquierda. La misma fue biopsiada y la anatomía patológica informó carcinoma medular de tiroides. El paciente presentaba múltiples metástasis hepáticas y pulmonares, motivo por el cual recibió tratamiento quimioterápico con cisplatino plus doxorrubicina (seis ciclos con respuesta completa, finalizando dicho esquema en marzo del 2002. En julio del 2002 se realizó tiroidectomía total más vaciamiento ganglionar izquierdo con resección de la glándula submaxilar. Durante la cirugía se lesionó la rama mentoniana del nervio cervicofacial, rama del VII par craneal. En septiembre del 2004 consultó por episodios de sudación durante las comidas en la región submaxilar izquierda. Se realizó prueba de Minor y luego se procedió a aplicar toxina botulínica tipo A en la región afectada, a razón de 2.5 UI por punto (cm², a un total de 17 puntos. El control a los 21 días demostró más de un 95% de efectividad en la resolución del SF. El efecto duró un año, y una segunda reinfiltración mostró similar respuesta. No hallamos descripciones anteriores de SF en región submaxilar; se presenta su tratamiento satisfactorio con toxina botulínica tipo A.A case of Frey syndrome (FS secondary to submaxillar gland exeresis is presented and the results of the treatment with botulinum toxin (BTX type A. FS is a condition of sweating cheek and preauricular area during mealtime as a sequel detected in about 20-60% of patients after parotidectomy. The clinical symptoms include swelling, flushing and hyperhidrosis. The treatment choice for this condition is intracutaneous injection of BTX type A which blocks acetylcholine release at the sweat glands. A 30-year-old man, with thyroid medullar carcinoma diagnosed in 2002 received 6 cicles of cisplatin plus doxorubicin previous to the thyroidectomy with anterolateral neck dissection. During surgery the left ramus marginalis mandibulae was damaged. Two years later the patient referred sweating in submaxillar region during meals. CT scan demonstrated the absence of left submaxillar gland. Minor's test disclosed the affected area and BTX type A was injected (2.5 U/cm²/17 points. A twenty-one-day control showed a 95% reduction of the affected skin area. Persistent efficacy was observed up to one year followup time when he was reinjected. The FS, also known as "gustatory hyperhidrosis", was probably first reported by M. Duphenix in 1757. Lucja Frey considered its physiopathology as a disorder of both sympathetic and parasympathetic innervation. In our case the FS was caused by a misdirected regeneration of postganglionic parasympathetic nerve fibers that arrised from the nervus lingualis rami ganglionares of the nervus trigeminus. After nerve injury the colinergic parasympathetic fibers seek out colinergic receptors -sympathetic receptors of the skin- innervating sweat glands and small skin vessels. All previous cases were located at masseter region post-parotidectomy. We have not found any description of FS in the submaxillary region. The self-assessed efficacy of the treatment with a hyperhidrosis disease severity scale revealed a very satisfied patient at 20 months follow-up after being injected twice with BTX type A.

Alejandra Baéz

2007-10-01

358

Molecular identification of Rickettsia parkeri infecting Amblyomma triste ticks in an area of Argentina where cases of rickettsiosis were diagnosed  

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Full Text Available Specimens of the hard tick Amblyomma triste were found infected with Rickettsia parkeri in an area of Argentina (General Lavalle, Buenos Aires Province where cases of human illness attributed to this microorganism have been reported. Molecular detection of R. parkeri was based on polymerase chain reactions that amplify a ca. 400-bp fragment of the 23S-5S intergenic spacer and a ca. 500-bp fragment of the gene encoding a 190-kDa outer membrane protein. Three (6.97% of 43 A. triste ticks were determined to be positive for R. parkeri. These results provide strong evidence that A. triste is the vector of R. parkeri in the study area. The findings of this work have epidemiological relevance because human parasitism by A. triste ticks has been frequently recorded in some riparian areas of Argentina and Uruguay and new cases of R. parkeri rickettsiosis might arise in the South American localities where humans are exposed to the bites of this tick species.

Gabriel Cicuttin

2013-02-01

359

Cutaneous findings in a case of Mediterranean spotless fever due to Rickettsia conorii, with gangrene of multiple toes.  

Science.gov (United States)

Mediterranean spotted fever (MSF) is a tick-borne disease caused by Rickettsia conorii conorii. Some rare cases present without a rash, and they are known as "spotless." This fact is important; although the mortality rates for MSF are low and generally range from 0% to 3%, the absence of a rash usually leads to a delay in the diagnosis and, therefore, an increase in the rates of morbidity and mortality. Necrosis of the digits is one of the complications of MSF that has occasionally been reported in the literature. However, very few reports have studied the morphological changes seen in the cutaneous necrotic lesions. In this report, we describe the morphological changes found through examining a biopsy taken from a necrotic cutaneous lesion in a 69-year-old man who had been diagnosed with Mediterranean spotless fever due to R. conorii. The main morphological changes included areas of collagen degeneration in the papillary dermis, necrotic eccrine glands, and hypodermal collagen with a smudged homogeneous appearance. PMID:23719481

Fernandez-Flores, Angel; De Cabo-Lopez, Erik; Diaz-Galvez, Francisco-Javier

2014-02-01

360

Molecular identification of Rickettsia parkeri infecting Amblyomma triste ticks in an area of Argentina where cases of rickettsiosis were diagnosed  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Specimens of the hard tick Amblyomma triste were found infected with Rickettsia parkeri in an area of Argentina (General Lavalle, Buenos Aires Province) where cases of human illness attributed to this microorganism have been reported. Molecular detection of R. parkeri was based on polymerase chain r [...] eactions that amplify a ca. 400-bp fragment of the 23S-5S intergenic spacer and a ca. 500-bp fragment of the gene encoding a 190-kDa outer membrane protein. Three (6.97%) of 43 A. triste ticks were determined to be positive for R. parkeri. These results provide strong evidence that A. triste is the vector of R. parkeri in the study area. The findings of this work have epidemiological relevance because human parasitism by A. triste ticks has been frequently recorded in some riparian areas of Argentina and Uruguay and new cases of R. parkeri rickettsiosis might arise in the South American localities where humans are exposed to the bites of this tick species.

Cicuttin, Gabriel; Nava, Santiago.

 
 
 
 
361

Dual exposure of Rickettsia typhi and Orientia tsutsugamushi in the field-collected Rattus rodents from Thailand.  

Science.gov (United States)

Field-collected rodents and fleas from ten provinces covering four regions of Thailand were investigated for possible rickettsial pathogen infections. The 257 trapped-rodents belonged to 12 species. Five species of Genus Rattus accounted for 93% of the total capture, of which Rattus exulans and Rattus norvegicus were the two major species caught. All flea specimens, removed from trapped rodents, were identified as Xenopsylla cheopis. The PCR technique was performed on ectoparasite specimens to detect the presence of murine typhus pathogen (Rickettsia typhi) and scrub typhus pathogen (Orientia tsutsugamushi). Thirteen flea specimens (2.6 %) were found to be positive for R. typhi but none for O. tsutsugamushi. An ELISA technique was used to detect the rodent's antibodies against R. typhi and O. tsutsugamushi. Sixty-one rodent serum samples (23.7%) were positive for R. typhi specific IgM, IgG, or both, while 47 of the samples (18.3%) were positive for O. tsutsugamushi. Twenty serum samples from R. norvegicus (7.8%) had detectable antibodies against both R. typhi and O. tsutsugamushi. Our findings revealed the existence of the dual infection of rickettsial pathogens in the same natural hosts. PMID:24820571

Chareonviriyaphap, Theeraphap; Leepitakrat, Warinpassorn; Lerdthusnee, Kriangkrai; Chao, Chien Chung; Ching, Wei Mei

2014-06-01

362

Histology, ultrastructure, and morphogenesis of a rickettsia-like organism causing disease in the oyster, Crassostrea ariakensis Gould.  

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Moribund specimens of the oyster, Crassostrea ariakensis Gould, aged 2-3 years were collected from Hailing Bay in Yangxi County of Guangdong Province from February to May and November to December in the years 2001, 2002, and 2003. A massive infection by an obligate intracellular prokaryote, specifically a rickettsia-like organism (RLO), was found. Here we report investigations of this RLO in the tissues of the oyster C. ariakensis Gould and describe the histology, ultrastructure, and morphogenesis of this pathogen in C. ariakensis Gould. Light microscopic observations of stained tissues revealed cytoplasmic inclusion bodies typical of prokaryote infection in about 87% (26/30) of the oysters. Most inclusions were observed in epithelial cells and connective tissues of the gill, mantle, and digestive gland of most of the infected oysters. The shape, size, and color of inclusions from different tissues were polymorphic. Electron microscopic examination of digestive gland, gill, and mantle tissues showed that the RLOs were intracytoplasmic. RLOs were often round, dumb-bell-shaped (undergoing binary fission), or occasionally rod-shaped and ranged from approximately 0.58 to 1.20microm in size. The organisms exhibited an ultrastructure characteristic of prokaryotic bacteria-like cells, including a trilaminar cell wall, electron-dense periplasmic ribosome zone, and a DNA nucleoid. Reproductive stages, including transverse binary fission, were observed by TEM. These stages were frequently observed within membrane-bound cytoplasmic vacuoles. Hexagonal phage-like particles in the cytoplasm of RLOs were also observed. PMID:15261771

Sun, Jingfeng; Wu, Xinzhong

2004-07-01

363

Primeiro caso de febre maculosa brasileira branda associada à artrite / First report of mild Brazilian spotted fever associated to arthritis  

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Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Descrevemos o primeiro caso brasileiro de Riquetsiose branda, agravada por monoartrite em joelho, em adulto jovem picado por carrapato na perna esquerda na região de Camburi, localizada no município de São Sebastião, sul da região costeira do estado de São Paulo, Mata Atlântica, Brasil. O paciente a [...] presentou uma escara de inoculação no local da picada do carrapato, associada ao aumento ganglionar em virilha esquerda, febre, poliartralgia, cefaleia e erupção macular. Vinte dias após o episódio da picada de carrapato, o paciente apresentou monoartrite em joelho direito. O diagnóstico de Riquetsiose branda foi estabelecido pela análise imunológica sequencial em amostras de soro e líquido sinovial, tendo sido empregada a técnica de imunofluorescência (IF) indireta para anticorpos reativos contra Rickettsia parkeri e Rickettsia rickettsii. A Riquetsiose branda é uma zoonose emergente, que deve ser investigada pelos médicos, incluindo reumatologistas, em pacientes que apresentem erupção macular, febre e, eventualmente, artrite, após visita ao sul da região costeira da Mata Atlântica no Brasil. Abstract in english We describe the first Brazilian case of mild Rickettsiosis, complicated by knee monoarthritis, in young adult bitten by a tick on his left leg in Camburi zone, located in São Sebastião municipality, southern coastal region of the State of São Paulo, in the Atlantic rainforest region, Brazil. The pat [...] ient developed inoculation eschar at the tick bite site associated with enlarged lymph nodes in the left groin, fever, polyarthralgia, headache and macular rash. Twenty days after tick bite episode, he displayed monoarthritis in his right knee. The diagnosis of mild Rickettsiosis was established by sequential immunological analysis in serum and synovial fluid, using the indirect immunofluorescence (IF) assay for antibodies reactive with Rickettsia parkeri and Rickettsia rickettsii. The mild Rickettsiosis is an emerging zoonosis, that must be investigated by physicians, including rheumatologists, in patients that present macular rash, fever and eventually arthritis, after visiting the southern coastal Atlantic rainforest region in Brazil.

Virgínia Lucia Nazario, Bonoldi; Roberta Gonçalves, Marangoni; Giancarla, Gauditano; Jonas, Moraes-Filho; Marcelo Bahia, Labruna; Natalino Hajime, Yoshinari.

364

Seroreactivity for spotted fever rickettsiae and co-infections with other tick-borne agents among habitants in central and southern Sweden  

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Patients seeking medical care with erythema migrans or flu-like symptoms after suspected or observed tick bite in the southeast of Sweden and previously investigated for Borrelia spp. and/or Anaplasma sp. were retrospectively examined for serological evidence of rickettsial infection (Study 1). Twenty of 206 patients had IgG and/or IgM antibodies to Rickettsia spp. equal to or higher than the cut-off titre of 1:64. Seven of these 20 patients showed seroconversion indicative of recent or curre...

Lindblom, A.; Wallme?nius, K.; Nordberg, M.; Forsberg, P.; Eliasson, I.; Pa?hlson, C.; Nilsson, K.

2013-01-01

365

Infecções humanas causadas por poxvirus relacionados ao vírus vaccinia no Brasil / Human infections caused by vaccinia-like poxviruses in Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese A partir de 1999, infecções humanas por Orthopoxvirus vem sendo observadas em pelo menos oito estados no país, com a formação de vesículas as quais evoluem para pústulas e crostas, principalmente nos membros superiores e face, após contacto com bovinos apresentando lesões semelhantes no úbere. Alem [...] das lesões na pele, foram descritas nos pacientes reações ganglionares axilares por vezes dolorosas, febre, cefaléia, fadiga, desidratação, anorexia, sudorese, artralgia e mialgia, evoluindo o quadro por três a quatro semanas. Lesão vulvar bem como transmissão intrafamiliar foram igualmente descritas. Estudos moleculares demonstraram que os poxvirus identificados são geneticamente relacionados a amostras do vírus vaccinia utilizadas no passado, nas campanhas de vacinação. Especimens clínicos de 80 infecções humanas foram estudados no laboratório e a infecção por orthopoxvirus confirmada em 68 casos. São apresentadas lesões observadas em pacientes bem como discutidas as implicações desta zoonose no Brasil. Abstract in english Since 1999, human infection caused by Orthopoxvirus has been observed in at least eight Brazilian states, with the presence of vesicles that evolve to pustules and crusts, especially on the hands, arms and face, after contact with cows showing comparable lesions on the udder. In addition to the skin [...] lesions, there have been descriptions of patients with axillary ganglionic reactions that are sometimes painful, along with fever, headache, fatigue, dehydration, anorexia, sudoresis, arthralgia and muscle pain. The condition evolves over a three to four-week period. Vulvar lesions and transmission within families have also been described. Molecular studies have shown that the poxviruses identified are genetic